LOCUS       DAOFSV010000001       133898 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.001, whole genome shotgun sequence.
ACCESSION   DAOFSV010000001 DAOFSV010000000
VERSION     DAOFSV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 133898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 133898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..133898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.001"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..151
                     /locus_tag="QBH27_000001"
     CDS             <1..151
                     /locus_tag="QBH27_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDQ7012384.1"
                     /translation="VALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGC
                     GPTAQ"
     gene            complement(259..1344)
                     /locus_tag="QBH27_000002"
     CDS             complement(259..1344)
                     /locus_tag="QBH27_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDQ7012385.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(1356..2660)
                     /locus_tag="QBH27_000003"
     CDS             complement(1356..2660)
                     /locus_tag="QBH27_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDQ7012386.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2672..3025)
                     /locus_tag="QBH27_000004"
     CDS             complement(2672..3025)
                     /locus_tag="QBH27_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDQ7012387.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3036..3914)
                     /locus_tag="QBH27_000005"
     CDS             complement(3036..3914)
                     /locus_tag="QBH27_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDQ7012388.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVGCTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3911..5137)
                     /locus_tag="QBH27_000006"
     CDS             complement(3911..5137)
                     /locus_tag="QBH27_000006"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDQ7012389.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            complement(5138..6301)
                     /locus_tag="QBH27_000007"
     CDS             complement(5138..6301)
                     /locus_tag="QBH27_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDQ7012390.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLVEYGVTHTEPGHALTGTIPANQRGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            complement(6385..6747)
                     /locus_tag="QBH27_000008"
     CDS             complement(6385..6747)
                     /locus_tag="QBH27_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDQ7012391.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6764..7669)
                     /locus_tag="QBH27_000009"
     CDS             complement(6764..7669)
                     /locus_tag="QBH27_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012392.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7831..8835)
                     /gene="trpS"
                     /locus_tag="QBH27_000010"
     CDS             complement(7831..8835)
                     /gene="trpS"
                     /locus_tag="QBH27_000010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDQ7012393.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8828..9586)
                     /gene="gph"
                     /locus_tag="QBH27_000011"
     CDS             complement(8828..9586)
                     /gene="gph"
                     /locus_tag="QBH27_000011"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDQ7012394.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9579..10256)
                     /gene="rpe"
                     /locus_tag="QBH27_000012"
     CDS             complement(9579..10256)
                     /gene="rpe"
                     /locus_tag="QBH27_000012"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDQ7012395.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10274..11110)
                     /gene="dam"
                     /locus_tag="QBH27_000013"
     CDS             complement(10274..11110)
                     /gene="dam"
                     /locus_tag="QBH27_000013"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ7012396.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11217..12503)
                     /gene="damX"
                     /locus_tag="QBH27_000014"
     CDS             complement(11217..12503)
                     /gene="damX"
                     /locus_tag="QBH27_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDQ7012397.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12595..13683)
                     /gene="aroB"
                     /locus_tag="QBH27_000015"
     CDS             complement(12595..13683)
                     /gene="aroB"
                     /locus_tag="QBH27_000015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDQ7012398.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHSIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGRFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13740..14261)
                     /gene="aroK"
                     /locus_tag="QBH27_000016"
     CDS             complement(13740..14261)
                     /gene="aroK"
                     /locus_tag="QBH27_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDQ7012399.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14662..15900)
                     /gene="hofQ"
                     /locus_tag="QBH27_000017"
     CDS             complement(14662..15900)
                     /gene="hofQ"
                     /locus_tag="QBH27_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDQ7012400.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15812..16216)
                     /gene="hofP"
                     /locus_tag="QBH27_000018"
     CDS             complement(15812..16216)
                     /gene="hofP"
                     /locus_tag="QBH27_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDQ7012401.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16206..16646)
                     /gene="hofO"
                     /locus_tag="QBH27_000019"
     CDS             complement(16206..16646)
                     /gene="hofO"
                     /locus_tag="QBH27_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDQ7012402.1"
                     /translation="MNMFFDWWFATSLRLRQLCWAVWLLVLVTLIFLSSTHQEERDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16630..17169)
                     /gene="hofN"
                     /locus_tag="QBH27_000020"
     CDS             complement(16630..17169)
                     /gene="hofN"
                     /locus_tag="QBH27_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDQ7012403.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLTRSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17169..17948)
                     /gene="hofM"
                     /locus_tag="QBH27_000021"
     CDS             complement(17169..17948)
                     /gene="hofM"
                     /locus_tag="QBH27_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDQ7012404.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            18068..20620
                     /gene="mrcA"
                     /locus_tag="QBH27_000022"
     CDS             18068..20620
                     /gene="mrcA"
                     /locus_tag="QBH27_000022"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDQ7012405.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20786..21346)
                     /gene="nudE"
                     /locus_tag="QBH27_000023"
     CDS             complement(20786..21346)
                     /gene="nudE"
                     /locus_tag="QBH27_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDQ7012406.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            21666..23801
                     /gene="igaA"
                     /locus_tag="QBH27_000024"
     CDS             21666..23801
                     /gene="igaA"
                     /locus_tag="QBH27_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDQ7012407.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMLFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23866..24534
                     /gene="yrfG"
                     /locus_tag="QBH27_000025"
     CDS             23866..24534
                     /gene="yrfG"
                     /locus_tag="QBH27_000025"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDQ7012408.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24545..24946
                     /gene="hslR"
                     /locus_tag="QBH27_000026"
     CDS             24545..24946
                     /gene="hslR"
                     /locus_tag="QBH27_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDQ7012409.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAVLLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24971..25849
                     /gene="hslO"
                     /locus_tag="QBH27_000027"
     CDS             24971..25849
                     /gene="hslO"
                     /locus_tag="QBH27_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDQ7012410.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25972..27180)
                     /locus_tag="QBH27_000028"
     CDS             complement(25972..27180)
                     /locus_tag="QBH27_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7012411.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEHKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            27188..27385
                     /locus_tag="QBH27_000029"
                     /pseudo
     CDS             27188..27385
                     /locus_tag="QBH27_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27483..29207)
                     /locus_tag="QBH27_000030"
     CDS             complement(27483..29207)
                     /locus_tag="QBH27_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDQ7012412.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGLLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            29585..31207
                     /gene="pckA"
                     /locus_tag="QBH27_000031"
                     /pseudo
     CDS             29585..31207
                     /gene="pckA"
                     /locus_tag="QBH27_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
     gene            complement(31284..32636)
                     /gene="envZ"
                     /locus_tag="QBH27_000032"
     CDS             complement(31284..32636)
                     /gene="envZ"
                     /locus_tag="QBH27_000032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDQ7012413.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(32633..33352)
                     /gene="ompR"
                     /locus_tag="QBH27_000033"
     CDS             complement(32633..33352)
                     /gene="ompR"
                     /locus_tag="QBH27_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDQ7012414.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            33580..34056
                     /gene="greB"
                     /locus_tag="QBH27_000034"
     CDS             33580..34056
                     /gene="greB"
                     /locus_tag="QBH27_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDQ7012415.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            34153..36474
                     /locus_tag="QBH27_000035"
     CDS             34153..36474
                     /locus_tag="QBH27_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDQ7012416.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMEHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREVQPAGNSAMMDALAAAMGKKR"
     gene            36912..37139
                     /gene="feoA"
                     /locus_tag="QBH27_000036"
     CDS             36912..37139
                     /gene="feoA"
                     /locus_tag="QBH27_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDQ7012417.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            37156..39477
                     /gene="feoB"
                     /locus_tag="QBH27_000037"
     CDS             37156..39477
                     /gene="feoB"
                     /locus_tag="QBH27_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDQ7012418.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQIASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            39488..39724
                     /gene="feoC"
                     /locus_tag="QBH27_000038"
     CDS             39488..39724
                     /gene="feoC"
                     /locus_tag="QBH27_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDQ7012419.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            39991..40353
                     /locus_tag="QBH27_000039"
                     /pseudo
     CDS             39991..40353
                     /locus_tag="QBH27_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(40382..41152)
                     /gene="bioH"
                     /locus_tag="QBH27_000040"
     CDS             complement(40382..41152)
                     /gene="bioH"
                     /locus_tag="QBH27_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDQ7012420.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            41190..41873
                     /gene="gntX"
                     /locus_tag="QBH27_000041"
     CDS             41190..41873
                     /gene="gntX"
                     /locus_tag="QBH27_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDQ7012421.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLLTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41932..42507
                     /gene="nfuA"
                     /locus_tag="QBH27_000042"
     CDS             41932..42507
                     /gene="nfuA"
                     /locus_tag="QBH27_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDQ7012422.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42871..44187
                     /gene="gntT"
                     /locus_tag="QBH27_000043"
     CDS             42871..44187
                     /gene="gntT"
                     /locus_tag="QBH27_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDQ7012423.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44297..46381)
                     /gene="malQ"
                     /locus_tag="QBH27_000044"
     CDS             complement(44297..46381)
                     /gene="malQ"
                     /locus_tag="QBH27_000044"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDQ7012424.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVIVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46391..48784)
                     /gene="malP"
                     /locus_tag="QBH27_000045"
     CDS             complement(46391..48784)
                     /gene="malP"
                     /locus_tag="QBH27_000045"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDQ7012425.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWVKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49407..52112
                     /gene="malT"
                     /locus_tag="QBH27_000046"
     CDS             49407..52112
                     /gene="malT"
                     /locus_tag="QBH27_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDQ7012426.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52155..52574)
                     /locus_tag="QBH27_000047"
                     /pseudo
     CDS             complement(52155..52574)
                     /locus_tag="QBH27_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="catalyzes the conversion of terminal3'-phosphate of
                     RNA to the 2',3'-cyclicphosphodiester; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            52504..52941
                     /locus_tag="QBH27_000048"
     CDS             52504..52941
                     /locus_tag="QBH27_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ7012427.1"
                     /translation="MFCSCKEKLPASVAISRSATWRGNFRELSASVTRMATFATSGRI
                     TLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSA
                     AGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(52923..53681)
                     /locus_tag="QBH27_000049"
     CDS             complement(52923..53681)
                     /locus_tag="QBH27_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDQ7012428.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(53698..54528)
                     /gene="glpG"
                     /locus_tag="QBH27_000050"
     CDS             complement(53698..54528)
                     /gene="glpG"
                     /locus_tag="QBH27_000050"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDQ7012429.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(54573..54899)
                     /gene="glpE"
                     /locus_tag="QBH27_000051"
     CDS             complement(54573..54899)
                     /gene="glpE"
                     /locus_tag="QBH27_000051"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDQ7012430.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55089..56594
                     /gene="glpD"
                     /locus_tag="QBH27_000052"
     CDS             55089..56594
                     /gene="glpD"
                     /locus_tag="QBH27_000052"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDQ7012431.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(56648..57253)
                     /locus_tag="QBH27_000053"
     CDS             complement(56648..57253)
                     /locus_tag="QBH27_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012432.1"
                     /translation="MNKIRLYIYPVIFGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(57256..58023)
                     /locus_tag="QBH27_000054"
     CDS             complement(57256..58023)
                     /locus_tag="QBH27_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012433.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPCLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGH"
     gene            complement(58126..59634)
                     /locus_tag="QBH27_000055"
     CDS             complement(58126..59634)
                     /locus_tag="QBH27_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDQ7012434.1"
                     /translation="MMQSGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEVC
                     NLLFTPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPEKREIAAWRQCFFLLPALLTL
                     VGWIIALHLADYQFRQMGSGWLANLMLSWQGVLLLSLISGDYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFLLLLLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGDDQEIQTWQRPEDSGSQTVMLSQVMKNVRMISP
                     QETEVASVMEGMINVVAEYRNTKNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNDKYPYIVDALMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMDKTLH"
     gene            complement(59759..62206)
                     /gene="glgP"
                     /locus_tag="QBH27_000056"
     CDS             complement(59759..62206)
                     /gene="glgP"
                     /locus_tag="QBH27_000056"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDQ7012435.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADYIWHIDPVRL"
     gene            complement(62225..63658)
                     /gene="glgA"
                     /locus_tag="QBH27_000057"
     CDS             complement(62225..63658)
                     /gene="glgA"
                     /locus_tag="QBH27_000057"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDQ7012436.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(63658..64953)
                     /gene="glgC"
                     /locus_tag="QBH27_000058"
     CDS             complement(63658..64953)
                     /gene="glgC"
                     /locus_tag="QBH27_000058"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDQ7012437.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPGKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(64971..66944)
                     /gene="glgX"
                     /locus_tag="QBH27_000059"
     CDS             complement(64971..66944)
                     /gene="glgX"
                     /locus_tag="QBH27_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDQ7012438.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVIDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(66941..69127)
                     /gene="glgB"
                     /locus_tag="QBH27_000060"
     CDS             complement(66941..69127)
                     /gene="glgB"
                     /locus_tag="QBH27_000060"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDQ7012439.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(69400..70503)
                     /gene="asd"
                     /locus_tag="QBH27_000061"
     CDS             complement(69400..70503)
                     /gene="asd"
                     /locus_tag="QBH27_000061"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDQ7012440.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            70695..71288
                     /gene="yhgN"
                     /locus_tag="QBH27_000062"
     CDS             70695..71288
                     /gene="yhgN"
                     /locus_tag="QBH27_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDQ7012441.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(71334..72629)
                     /locus_tag="QBH27_000063"
     CDS             complement(71334..72629)
                     /locus_tag="QBH27_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDQ7012442.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(72536..74632)
                     /locus_tag="QBH27_000064"
     CDS             complement(72536..74632)
                     /locus_tag="QBH27_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDQ7012443.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSDLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(74773..76113)
                     /gene="gntU"
                     /locus_tag="QBH27_000065"
     CDS             complement(74773..76113)
                     /gene="gntU"
                     /locus_tag="QBH27_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDQ7012444.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76117..76644)
                     /gene="gntK"
                     /locus_tag="QBH27_000066"
     CDS             complement(76117..76644)
                     /gene="gntK"
                     /locus_tag="QBH27_000066"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDQ7012445.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(76783..77778)
                     /gene="gntR"
                     /locus_tag="QBH27_000067"
     CDS             complement(76783..77778)
                     /gene="gntR"
                     /locus_tag="QBH27_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDQ7012446.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(77871..78566)
                     /gene="yhhW"
                     /locus_tag="QBH27_000068"
     CDS             complement(77871..78566)
                     /gene="yhhW"
                     /locus_tag="QBH27_000068"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDQ7012447.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRIWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(78689..79726)
                     /locus_tag="QBH27_000069"
     CDS             complement(78689..79726)
                     /locus_tag="QBH27_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ7012448.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVSLAR"
     gene            80060..80548
                     /gene="yhhY"
                     /locus_tag="QBH27_000070"
     CDS             80060..80548
                     /gene="yhhY"
                     /locus_tag="QBH27_000070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ7012449.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTAHPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(80609..82342)
                     /gene="ggt"
                     /locus_tag="QBH27_000071"
     CDS             complement(80609..82342)
                     /gene="ggt"
                     /locus_tag="QBH27_000071"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDQ7012450.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDVTATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNTDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPSVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            82462..82902
                     /locus_tag="QBH27_000072"
     CDS             82462..82902
                     /locus_tag="QBH27_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDQ7012451.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(82889..83632)
                     /gene="ugpQ"
                     /locus_tag="QBH27_000073"
     CDS             complement(82889..83632)
                     /gene="ugpQ"
                     /locus_tag="QBH27_000073"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDQ7012452.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTDTGPLTGKMVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(83629..84699)
                     /gene="ugpC"
                     /locus_tag="QBH27_000074"
     CDS             complement(83629..84699)
                     /gene="ugpC"
                     /locus_tag="QBH27_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDQ7012453.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYVGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGHWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(84701..85546)
                     /gene="ugpE"
                     /locus_tag="QBH27_000075"
     CDS             complement(84701..85546)
                     /gene="ugpE"
                     /locus_tag="QBH27_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDQ7012454.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(85543..86430)
                     /gene="ugpA"
                     /locus_tag="QBH27_000076"
     CDS             complement(85543..86430)
                     /gene="ugpA"
                     /locus_tag="QBH27_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDQ7012455.1"
                     /translation="MPSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(86528..87844)
                     /gene="ugpB"
                     /locus_tag="QBH27_000077"
     CDS             complement(86528..87844)
                     /gene="ugpB"
                     /locus_tag="QBH27_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDQ7012456.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEARIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(88241..88954)
                     /gene="livF"
                     /locus_tag="QBH27_000078"
     CDS             complement(88241..88954)
                     /gene="livF"
                     /locus_tag="QBH27_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDQ7012457.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(88956..89723)
                     /gene="livG"
                     /locus_tag="QBH27_000079"
     CDS             complement(88956..89723)
                     /gene="livG"
                     /locus_tag="QBH27_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDQ7012458.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGMPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(89720..90997)
                     /gene="livM"
                     /locus_tag="QBH27_000080"
     CDS             complement(89720..90997)
                     /gene="livM"
                     /locus_tag="QBH27_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDQ7012459.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(90994..91920)
                     /gene="livH"
                     /locus_tag="QBH27_000081"
     CDS             complement(90994..91920)
                     /gene="livH"
                     /locus_tag="QBH27_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDQ7012460.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(91968..93077)
                     /gene="livK"
                     /locus_tag="QBH27_000082"
     CDS             complement(91968..93077)
                     /gene="livK"
                     /locus_tag="QBH27_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDQ7012461.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            93500..93883
                     /gene="panM"
                     /locus_tag="QBH27_000083"
     CDS             93500..93883
                     /gene="panM"
                     /locus_tag="QBH27_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDQ7012462.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
     gene            complement(93880..94407)
                     /locus_tag="QBH27_000084"
     CDS             complement(93880..94407)
                     /locus_tag="QBH27_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ7012463.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTPDD"
     gene            complement(94414..94680)
                     /locus_tag="QBH27_000085"
     CDS             complement(94414..94680)
                     /locus_tag="QBH27_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDQ7012464.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            complement(94829..95932)
                     /gene="livJ"
                     /locus_tag="QBH27_000086"
     CDS             complement(94829..95932)
                     /gene="livJ"
                     /locus_tag="QBH27_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDQ7012465.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(96203..97057)
                     /gene="rpoH"
                     /locus_tag="QBH27_000087"
     CDS             complement(96203..97057)
                     /gene="rpoH"
                     /locus_tag="QBH27_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDQ7012466.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(97302..98360)
                     /gene="ftsX"
                     /locus_tag="QBH27_000088"
     CDS             complement(97302..98360)
                     /gene="ftsX"
                     /locus_tag="QBH27_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDQ7012467.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYASHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(98353..99021)
                     /gene="ftsE"
                     /locus_tag="QBH27_000089"
     CDS             complement(98353..99021)
                     /gene="ftsE"
                     /locus_tag="QBH27_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDQ7012468.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(99024..100520)
                     /gene="ftsY"
                     /locus_tag="QBH27_000090"
     CDS             complement(99024..100520)
                     /gene="ftsY"
                     /locus_tag="QBH27_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDQ7012469.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            100670..101266
                     /gene="rsmD"
                     /locus_tag="QBH27_000091"
     CDS             100670..101266
                     /gene="rsmD"
                     /locus_tag="QBH27_000091"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDQ7012470.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            101256..101525
                     /locus_tag="QBH27_000092"
     CDS             101256..101525
                     /locus_tag="QBH27_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDQ7012471.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(101528..101887)
                     /locus_tag="QBH27_000093"
     CDS             complement(101528..101887)
                     /locus_tag="QBH27_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDQ7012472.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            102028..102654
                     /locus_tag="QBH27_000094"
     CDS             102028..102654
                     /locus_tag="QBH27_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDQ7012473.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            102728..104926
                     /gene="zntA"
                     /locus_tag="QBH27_000095"
     CDS             102728..104926
                     /gene="zntA"
                     /locus_tag="QBH27_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDQ7012474.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAATGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLSNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(105028..105273)
                     /gene="tusA"
                     /locus_tag="QBH27_000096"
     CDS             complement(105028..105273)
                     /gene="tusA"
                     /locus_tag="QBH27_000096"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDQ7012475.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            105483..106148
                     /locus_tag="QBH27_000097"
     CDS             105483..106148
                     /locus_tag="QBH27_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDQ7012476.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            106221..106778
                     /gene="dcrB"
                     /locus_tag="QBH27_000098"
     CDS             106221..106778
                     /gene="dcrB"
                     /locus_tag="QBH27_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDQ7012477.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(106782..108032)
                     /locus_tag="QBH27_000099"
     CDS             complement(106782..108032)
                     /locus_tag="QBH27_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7012478.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            108131..109180
                     /locus_tag="QBH27_000100"
     CDS             108131..109180
                     /locus_tag="QBH27_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDQ7012479.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            109235..109822
                     /gene="acpT"
                     /locus_tag="QBH27_000101"
     CDS             109235..109822
                     /gene="acpT"
                     /locus_tag="QBH27_000101"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDQ7012480.1"
                     /translation="MYRIVLGKVSTLSAAPLPLGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            109933..111507
                     /gene="nikA"
                     /locus_tag="QBH27_000102"
     CDS             109933..111507
                     /gene="nikA"
                     /locus_tag="QBH27_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDQ7012481.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            111507..112451
                     /gene="nikB"
                     /locus_tag="QBH27_000103"
     CDS             111507..112451
                     /gene="nikB"
                     /locus_tag="QBH27_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDQ7012482.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            112448..113281
                     /gene="nikC"
                     /locus_tag="QBH27_000104"
     CDS             112448..113281
                     /gene="nikC"
                     /locus_tag="QBH27_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDQ7012483.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            113281..114045
                     /gene="nikD"
                     /locus_tag="QBH27_000105"
     CDS             113281..114045
                     /gene="nikD"
                     /locus_tag="QBH27_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDQ7012484.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            114042..114848
                     /gene="nikE"
                     /locus_tag="QBH27_000106"
     CDS             114042..114848
                     /gene="nikE"
                     /locus_tag="QBH27_000106"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDQ7012485.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARTSEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            114854..115255
                     /gene="nikR"
                     /locus_tag="QBH27_000107"
     CDS             114854..115255
                     /gene="nikR"
                     /locus_tag="QBH27_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDQ7012486.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            115454..116200
                     /locus_tag="QBH27_000108"
     CDS             115454..116200
                     /locus_tag="QBH27_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ7012487.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            116225..116698
                     /locus_tag="QBH27_000109"
     CDS             116225..116698
                     /locus_tag="QBH27_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ7012488.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIRQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDPDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMCALLTMETTEIHTAILNKMKERGEI"
     gene            116695..116976
                     /locus_tag="QBH27_000110"
     CDS             116695..116976
                     /locus_tag="QBH27_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDQ7012489.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            117053..118411
                     /locus_tag="QBH27_000111"
     CDS             117053..118411
                     /locus_tag="QBH27_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDQ7012490.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSSGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGIFLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIALVPVIALVLYGIFRMTRSTV
                     YA"
     gene            118404..119912
                     /locus_tag="QBH27_000112"
     CDS             118404..119912
                     /locus_tag="QBH27_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDQ7012491.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKATALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVD"
     gene            119902..120171
                     /locus_tag="QBH27_000113"
     CDS             119902..120171
                     /locus_tag="QBH27_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDQ7012492.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKCSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            120203..121054
                     /locus_tag="QBH27_000114"
     CDS             120203..121054
                     /locus_tag="QBH27_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HDQ7012493.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRSVK"
     gene            complement(121163..122287)
                     /locus_tag="QBH27_000115"
     CDS             complement(121163..122287)
                     /locus_tag="QBH27_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7012494.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(122287..125022)
                     /gene="rbbA"
                     /locus_tag="QBH27_000116"
     CDS             complement(122287..125022)
                     /gene="rbbA"
                     /locus_tag="QBH27_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDQ7012495.1"
                     /translation="MMHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(125019..126086)
                     /locus_tag="QBH27_000117"
     CDS             complement(125019..126086)
                     /locus_tag="QBH27_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ7012496.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            126821..127873
                     /locus_tag="QBH27_000118"
     CDS             126821..127873
                     /locus_tag="QBH27_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HDQ7012497.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNKLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(127960..129153)
                     /locus_tag="QBH27_000119"
     CDS             complement(127960..129153)
                     /locus_tag="QBH27_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDQ7012498.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            129385..130884
                     /gene="pitA"
                     /locus_tag="QBH27_000120"
     CDS             129385..130884
                     /gene="pitA"
                     /locus_tag="QBH27_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDQ7012499.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(130955..131290)
                     /gene="uspB"
                     /locus_tag="QBH27_000121"
     CDS             complement(130955..131290)
                     /gene="uspB"
                     /locus_tag="QBH27_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDQ7012500.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            131681..132115
                     /gene="uspA"
                     /locus_tag="QBH27_000122"
     CDS             131681..132115
                     /gene="uspA"
                     /locus_tag="QBH27_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDQ7012501.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            132432..>133898
                     /gene="dtpB"
                     /locus_tag="QBH27_000123"
     CDS             132432..>133898
                     /gene="dtpB"
                     /locus_tag="QBH27_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDQ7012502.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     QLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
BASE COUNT        31926 a        36387 c        33746 g        31839 t
ORIGIN      
        1 agttgcactg gtaggcgcag tactggctac gttaactgca tgtactggtc atattgaaaa
       61 ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta tttctaaaat
      121 cattggcggt tgcggtccga ctgcacagta agtctctgac agaaatcggc taacgccggt
      181 ttaattgcct gacgccctcg gagttatccg agggcgtatt tgtttctgca ttcttttcga
      241 aaatatcgtt tacaacgatt aaatgctggc aatactttca cgcaagatcc gcaatatcgt
      301 ctcttcaccg ccgcgatttg ggttaatgcg aatcgcgcaa tgttctaact gcggattcgc
      361 ctggcgaaac gttccggaaa gtcgataaaa gagcggcggg atttcatatt tcgactctgc
      421 accaaccggg taaggcaagg caccgagttt ctgcgcctct tccagcactc tggcggcaat
      481 cggctgatga aactccacaa tcaatacctt cgactgtgca ttggcaatga cggcgctttt
      541 cacttccgcc accgcgccgc cgttaagcaa agccagcaac cgttcagata cccccgcctg
      601 caccgcgtgc atcactggcg cgagcaccag accacgcaac acctctaatg cctgcgcccc
      661 ctgaatctga ctgccgccgg aatagagcgt ggcgcgaatc cggctaataa catcggcatc
      721 gcctaccacc gcaccaacgc cctctggccc aaatagcttg aagcaggaaa atgtcgacac
      781 attcgccccg cattcgcagc cgattcgcgc taccttcatc accgcatagt tgtcatcggt
      841 taacgctgga acacctgccg cgcgcaacgt tgccagcacg tctgccagca catagcggtc
      901 ctgcggctgc tggcgcgtat gctgcaccag cgcggcatca ggttgttgct cgtcaacaac
      961 ctgcttcagt gccgacagat cattgaaatc agccgtaatg agcgttagcc ccatctgctc
     1021 aataatgacg cgcgtcgttg gataaaccgg cgcgtcatgc accagaagac gctgccccga
     1081 tttgagcaaa gctgccagcg ccgcacggat cgcgccagta cccgcgccct gtaccagcgc
     1141 cgcggcctgt gcgtgaaacg cgtcagccag cacctgttcc acacgctggg taatgcgggg
     1201 ctgatttagc cccggcgtta agcccaaatc accgcaggta agaaactcgc tgccaggaaa
     1261 atgacggcaa atcgtgtcca ccagcgcaaa ctgcttttgc tgcgcctcaa taagcgtcag
     1321 gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa caggccaagc
     1381 cagtaaagaa cattcagcag gatgccggtg atcattactg ccacgaccgg tgccgccatt
     1441 ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc gatagagaat
     1501 ccggtatagc ccgccatttt gattgctgca aagatcgaac cgaccagcaa cgccacttcc
     1561 atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgtaccga cgggtagcgc
     1621 cccaaccatt tgccgataga acgaagcagc aagacttccg ccgaaataac cactgcgcct
     1681 aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac aaaggtaaag
     1741 cccgcaactg catacacgcc ggtggctaac gccgtggtgg caatcaacgg cacaaaaccc
     1801 agcccgcgca taaattctgc cagtgccgcc tggttgatta gtgtttgcga ttgttccggc
     1861 gttacgcctg cggagtacgc tttctccagt gtgaagatcg acacttcact accggcgaaa
     1921 atcttcatgc tggcaacggc agcaatcaat gcacccacga tggcgatata cggtaagttt
     1981 ttgataatcc gtgatgtacg ctcttcaaac accgacatac cgctggcatc gatatcattt
     2041 tcatcacgat gacgcaggtc atgagttatt gcgatcccca gcagcatcac catgccaata
     2101 aagatttcga tggattcagg gttaagatgt gggaaatagc gtactaccac cacacgagtc
     2161 atcagtacca ccacggcggc gatcagactt tgcttccagc caaactggta gaaaatcgcc
     2221 accaacggga acagtgcaaa agctgaaacc accggcgagc ttaattcccc caggctacct
     2281 aatacatcca ccggtagcgc ggtcagcagc tggtttactg gcaacagaca ggtgaggatc
     2341 aatattcccc agatagcacc aagaccaaac gccatcaggc tgtttatcgc cagtacgccg
     2401 aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt cagggtaaaa
     2461 gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatgct ccccgcctcg
     2521 cgacggttca tatagccttc aatcagttgt ggcaggatcg ggcggatacc gtcatgaaaa
     2581 acggccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc caccacgata
     2641 atctgaatat acagatccat agtcgtgcct tttattttag atggttaatc agcatcggaa
     2701 tcgcgtgttc aacgtgctca acggaaaggc caaacgccac ttttccttca gcgatcattt
     2761 tggcgatatg ttcgtctttc gctttaatgc ccggcttggc aatggtgcaa cttttgttat
     2821 agccaatcac cgcgatagca atcgacaagg ctgcgcccgc accggtatta cacgcgccga
     2881 tgtagtaatc cagttgtccg gatttcactt tcattgccgc ttccatgtcg ttgtgaatga
     2941 aaacttcaaa acagccagga gccgtcgctt caatggtttt tttaatctgc tcacgctgta
     3001 agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg agagggattt
     3061 tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa ttgcggaata
     3121 aaggtggtca ataaaaagtc atagccataa ccaccgttgg cttttaaatg agagcgccgc
     3181 gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag cgcatggagc
     3241 atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttgc cgatggtgtc gaactgcaca
     3301 tacgcgccga gatcgatcat cttcaaaatg ttgtcgagat tgtctttcag atcgcagtgg
     3361 ccgacggtga cgcgcgaaag atcaactccg tgggcttgca acagagccag ttgctccagc
     3421 cccatcgtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt atgcgccagc
     3481 gcggcggcaa taaacacctt ctcttccagc gacgtaatct tcccttcgct ggagccgatc
     3541 tccgcgataa ttccggcttt caggtcagtg ccatcgatac cctgctcaat ttcatcgacc
     3601 atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa aaacgcgtcc
     3661 tggtaataac cggtacagcc caccacgttg atcccggttt cgcgcattac atcgagcata
     3721 aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg cacgccccgg
     3781 gccatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg gcagtccacg
     3841 ttgtttttaa agccggaaag atcaatatgc agatgctcat gggcgagggt gtagcccgtc
     3901 ggatcaaaac tcatagggtg tctcctgcaa accggaagga ggaaagaaaa gagcgaccat
     3961 tttgtggcgg tggcgcacgg aaaaattcac acacggtagc ccccacatca gaaagcgtgg
     4021 tgcgcacacc gagctgcgta gcgaccagtc cttgctgata aaccagcact ggcaccactt
     4081 cgcgggtatg gtggctgtga ccgatggtcg gatcgttacc gtgatccgcc atcacgacca
     4141 ggcaatcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca acgacctgca
     4201 aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt tcctggatat
     4261 tggtacaaat aaacgccgtc ggataggtgt taaattcgtt gagggtgata tccataatcc
     4321 gctggctatc caccaggttt tgccagctca cgccataagg attgctgacg atatctgcca
     4381 ccttacccac cagcacggtt ggcacgcccg cttcatacag tttttgcggc acctgcactt
     4441 tttcatcgac accgtagccc atatgtacga cctggaaacc gttgtcatag gcgccagaac
     4501 gcggcgcatt gataccaata aagcgccctt ctttgctttc tgcggcatcg aggatgcact
     4561 ggctgtcggt taacaggcca ccaaatgtaa tgacccgacc gacctgtacc tgctcacgca
     4621 cgatacgacc cattttgatt gcgtcgtcaa aagagatcac agagagattg gcggtgatgt
     4681 tatagacctg acctaaatcc gcctcgagat tatcgccaat cgcaaccgcc tgattgaccc
     4741 acagaaattg cagatcatcg ccacgacgct caacctgcca gccagcggaa actaatgcct
     4801 gctcaacacg gtcaatcaca tcacgaaagg gcatccgcag cggcggtaat gggcgcgtac
     4861 ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc tctgccacgc
     4921 cccaggttgc ggagtctgac ggctgcatat cgccaggcgc ataacccaat gcgttgatta
     4981 gccccagcgt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg atgtgaccac
     5041 aggtattcgc cccggcatcc tgcggacgca ccaacgtgac atctttcatt gcccctacgc
     5101 caaagctatc aatcactaac accacaaatc gcgccattta cgcctccaga gggttgccga
     5161 ggctgtcata acgaccaact atttctggtt cgccatggtg aataccggac accaacacca
     5221 catcgctacg ggtgataaaa atctgcgtgc gaaaacagag caccactgca ctgcttaccg
     5281 gatgctcgcc tgccagcggc agggtgtagt cgatactgct gtcatccacg gcgttgagat
     5341 atgtttcagt aattctttga ttttctggcg taaacaccag cgcatgttgc gcgtgaccac
     5401 gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg gagatttcac
     5461 ttaaccagag catcgcgata cgttcaggct gatcgccccg ctggtttgcc ggaatagtgc
     5521 ccgtcagcgc atgaccgggt tcggtatgag tcacaccgta ttccaccagc aatggcagcg
     5581 acgtgcagct ggtcgctgaa ggcgcgttca gttgctcaat cgcaatacca gatttcgcca
     5641 gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt cggcaaaact ttcccggcag
     5701 cctcatccca aagcaggcaa gggaaatggg taagtcccgc caaatgtagc cccggcagat
     5761 tctggatttc agcaaccacc tcgtgcagcg aatgctgtac aaaaccgctc tcctgccccg
     5821 gataaagaaa atcatcgtcg ctataaactt tgagcagcac agactgcaca cgtccggcct
     5881 tcaccgctgc cgcagaaatt tcccgcgctt tgtcgagggt aaacacagtg atgacgtcgg
     5941 ttccctgttc aacggcgtca gaaacctgat gacaagggat ttgtaccaga tgcccctgat
     6001 gcgctaccgg caaaccggcg cggcgcatga ctcgcgcctc tttgtaatcc accgccacaa
     6061 tgccgctgta gcccagagcc agcagttttt ctgccagcca cggattacga ccgaattgtt
     6121 tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatcagc cgcttgccgt
     6181 tttccagaac ttgatccacg tcgatcaccc aactgtccgg ggcgatcttg ccctgctgcc
     6241 acaaacttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct tctacaaaca
     6301 tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat atttaggttg
     6361 tacgacgtgg atttatcggg agcttcaaac ctcttcgtta gcagccatcc ataatccata
     6421 aaggttggcc agcaaatagc cttcttcaca cgggttaact tccagtgcaa attccttcaa
     6481 caatacctga tgcaattgca caacggcttg ccagtggctg gattgcgcca gttccgccag
     6541 caattcgtca tccagcggct ctatttcttc accacggcga ctgcgcatca gtgcactcgc
     6601 catatgtgtc atcgccatcg ttccttgctc actgcgcacc ggtagatggc actctgtttc
     6661 cagtacattg acgacctgca tcatgccttt gcagatgtcc ttatcaataa cgcctgcatc
     6721 gcaaagcagg ttgagtctgg tttccatatc agatgccttt cgtttaataa ctgggttcct
     6781 gttccccact gactactcgc tgttgatgag caagcagggc atgtttatca acaactgcac
     6841 gcagcagttg ttgcattggg taatcatcga ctcgcgtcag gaccacggct tcggtggcct
     6901 gtaaaaatcg cggatcgtct gtgagcggcg tcgcttctaa tcccagcatg gtcagttcgt
     6961 tttccgccac tacgttccag ataaccgcgt caacatcgcc tttaacaatg cgttgtaaac
     7021 tctcgtgata agagagatcg actcgttcca catcactatc gccaaaaaag acatcggtca
     7081 tgattttctg atccgccgaa cggttatcca gccccacgcg cttcacgttt gcggactcgc
     7141 ctttacggca aatcaactgg tgctcgccaa cgtaggtatg cggtcccaac tccagcgcga
     7201 ggcataatcc tttttgcgtg agataacttt ccgccgccag tcgcgaaacc accgccatgt
     7261 catacacgcc gttaagtaaa cactccacgc gaatatccgc gccgcgcata tgcgcatagt
     7321 aaaaaggaat gccatcaaac tgggctttca atccgctcgc caggccttcg tacaaacggg
     7381 tatagggcaa gggcatcgca cataccacgt tgttgatatc cacatgagtc agcaatgctt
     7441 tgttatccat ctcaaccaga taactgccat tgcgcccacg gcgttcaatc cgtatcgctc
     7501 cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aaccgatgaa cggcattcag
     7561 ttgccagctc atctatggtt ttcagtcgat taccgcactt ttcacccaac aaataacggg
     7621 ccagtgtcgt gataacgacg ccttcttttt tgataaacgt tcgacgcata atatattttc
     7681 aataaaatga atgtttatat cttcatgaat ttgaagataa ggcgcaacag cgagatgtgg
     7741 aaacggcgag gcgcttcaca ttttttcgga ttattgcaaa tgaggataaa aaaaccgggt
     7801 ttccccggtt tttttggcat taatgttaaa ttacggcttc gccacaaaac caatcgcttc
     7861 gtacaccgct tttagcgtac gggaagcgtg tgcgctggct ttttctgcgc cgtctttcat
     7921 cacctgttgc aggaaggctt catcgttgcg gaaacggtgg taacgttcct gcaattcagt
     7981 cagcataccg gaaacggcat cagccacttc acctttcaga tgaccataca tcttgccttc
     8041 gaactgtttt tccagttccg ggatgctctg gcccgttacc gcagaaagga tatccagcag
     8101 gttggaaacg cccgctttgt tctgcacatc gtagcgaact actggcggct cgtcggagtc
     8161 agtgaccgcg cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac
     8221 gttattgcgg ttatcgtcag acttggacat tttcttggtc ggctccagca gcgacattac
     8281 gcgcgcgcca gatttcggaa taaacggctc tggcacctta aagatatcgc cgtacagcgc
     8341 gttgaaacgc tgggcgatat cgcggctcag ttccaggtgc tgtttctggt cttcacccac
     8401 cggtaccaga tttgtttgat acagcaggat gtccgctgcc atcagcaccg gatagtcaaa
     8461 cagaccagcg ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg
     8521 gctcagttcg ccgaagtagg tatagcagtt caatgcccag cctaactgcg catgttccgg
     8581 cacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa
     8641 cgccagcgta tccagcgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc
     8701 atgttggtca acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg
     8761 cagcgcaccc atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac
     8821 gatgggctta gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca
     8881 gaaggtcatt tatagactga taaattacat caggctggct gagatcgata gcctcgccgt
     8941 agttatatcc gtaggttaag ccaactgatg ggcaacctgc cgcttttgct gcctgaatat
     9001 cattgcgtga gtcgccaaca aacagcatct gttgtggagc aatgcccatc cgttcagcca
     9061 ccagtaacag cgggtccgga tgcggttttt tgttttgcac atcatcacca ccaatcacca
     9121 cgctgaagta tttggcgata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt
     9181 tgttggtgac caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa
     9241 catgcgggaa caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt
     9301 tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca
     9361 ttgttttacg cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa
     9421 tatctgcgcc gttaccaatc caggtaataa cgcgttcttc acctgcgaca ggcaactcca
     9481 gcgcatacag cgccatatct accgcagcag caagaccggg tgcactgtca accagcgtac
     9541 cgtcgagatc aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc
     9601 agttcactgc gcatttcatc aatgactttt ttgtagtctg gctggtcgaa gattgccgaa
     9661 ccagcaacaa acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg
     9721 ccaccgtcca cttccagtcg aatgtcaaag ccagactcgt cgatacgacg acgtacttcg
     9781 cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg gttaacagac
     9841 atcagcagga tcacatccag cttgtccatt acataatcca gatagctcaa tggtgtcgcc
     9901 gggttaaata ccagacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca
     9961 acatgctcgg aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca
    10021 ggcacaatgc gatcgacggg tttcaccatc agatgtacgt cgataggggc ggtaatgccg
    10081 tagttacgca aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta
    10141 tccatgacgt caaaatgcac gacatctgca cccgctgcca gggcttttgc ggtatcttca
    10201 cccaggcggg caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc
    10261 ttctccttga gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca
    10321 gttcgtccac ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga
    10381 catgcaattt tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca
    10441 gcactggaat atggcgatca accagacctt cagcgatctc cgccagatgc gcttgttgtt
    10501 caagcgtaaa actgtttgtg tgatacgccg taaagttggc agtcgcagac agcggcgcat
    10561 aaggcggatc gcaatagacg acagatgcat catctgcgcg cgccatgcta tcggcgtaag
    10621 actcacaata gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg
    10681 ggaaataggg ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac
    10741 gacacaggcc gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg
    10801 gatcctggct tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt
    10861 cgggaacaaa tagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt
    10921 tatagagact gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt
    10981 tgagaaacac cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat
    11041 gccgtttaat atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat
    11101 ttttcttcat gctgactaac taattacacc ttctccggct gtggagaaag ctccgacagc
    11161 atccagcgct tcaggcaact cccgcaatga taaaccattg cgggagataa ccttgattac
    11221 ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctggacatct
    11281 gctggcaatg tagataccgc ttttttcgcc tcttctttcg aagcatacac gccagaaacc
    11341 aggacatacc acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc
    11401 tctttcttcg cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg
    11461 taatggctgg acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg
    11521 gctggtgttg ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc
    11581 tcttttggtg ctggcgtaga agtcgcagcc ggtgcctttg tgctcgccac aggggcagct
    11641 ggttcagttc gcttcggcgt ctgtgcgact ggcttcggtt ccgttttcac ggttgcttgc
    11701 ggttttttcg gttcaatcac cgcctgctga cgagctgggc gcgtagtggc cggacgttca
    11761 gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt
    11821 gcgggttcgg tcggcaatgt agaattgacc gccacattgt tcaactgttg ctgattttgt
    11881 ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt
    11941 gccgccgggg tttgcccttg agtcggcgta gaagagatcg gcggcagaga aacatcctgc
    12001 tgagtatttt ccgcagacgt ggttcccggc gctggctgca cgccattggc ctgatcggtc
    12061 gcattgcctg caagatcaat actcttctcg ccagacgcag tttgatcgct ggaagaggtc
    12121 gagggggctt ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg
    12181 acacccatca tcatatactg acgagaagcg ggtttactgg ctgctttttt acgcttacgc
    12241 ggacgacgct ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct
    12301 tcgatttccg gttcctcatt acgcgctttt tgcgcacgag tcggacggcg atcgtcagtg
    12361 tcatcaagtt caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa
    12421 cgttcagaag attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg
    12481 tcttctggtt tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg
    12541 caattaacct gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc
    12601 tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc
    12661 actcttacca attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc
    12721 acgcagcata tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa
    12781 cccagcccgc gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa accgcccgag
    12841 acgttccgac gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca
    12901 attgccatac cccatttcag cttcaatgga atgaccaaag gtgtgtccca gattcagtaa
    12961 agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact tctgctttca gctcacaaca
    13021 acggcgaata cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc
    13081 ttccagccag ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag
    13141 ccccgacgct aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga
    13201 agcgggctgg tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt
    13261 gccgccaacg gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgtac
    13321 accgcgctga taactcgccg ccgcgaagcc ggtcagatca cccactacgc cgccgccaag
    13381 cgccaccaga gtagtatcgc gaccatgcgg cttttgtaac aatgccgtaa agacggtatc
    13441 gagtacagcc aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac
    13501 acccgcctgt tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgtt
    13561 ggtgaccaac atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa
    13621 caaaccagat gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc
    13681 cataacgcga catccacctt aattactgta cccgcagacg agtgtatata aagccagaat
    13741 tagttgcttt ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca
    13801 gtacgaatgg tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct
    13861 tccagaactt cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc
    13921 gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa
    13981 agacggttac gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc
    14041 tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct
    14101 tctaaatcga aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa
    14161 tcgtaaaatt ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca
    14221 cccataggcc caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga
    14281 ctattcgtta atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc
    14341 tcataagata ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa
    14401 ctgaataaat cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc
    14461 accgctctgg cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc
    14521 tgaatgataa atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt
    14581 actccttgta tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcgtaaaaa
    14641 cattaccgct tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac
    14701 taactcgcgt cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat
    14761 gtcgccaagc aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc
    14821 cagcgccaac gtttctccgc ttttgacctc gacctgcgtt tcgatctcct gcttatcaat
    14881 cgccagcact tcgccatctg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg
    14941 taatttcagc cggatgcgac ctttttgtaa caccgtgggc gtgacttcca tccccaggac
    15001 ggcctcttta aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat
    15061 ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc
    15121 gataatatcc agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gtaaacgtcc
    15181 gttgatgcgt ccaatgttaa aaccgatatg cgttgtcgcc gtcgctacgg agaggtcgct
    15241 gccaagcgtg gtgacttgcc caacgccacc agcttgttgc gcatcggcca gcgtccattt
    15301 cacacctaac tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac
    15361 ctgcccgacc ggcagatcca tttgcgatac ccactgttca agcgtgctta acgccgtttt
    15421 gttatctcgc agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact
    15481 cagtagcttc tcccccgctt tcgccagctc tccggcgtcg gcgtattgca gagtaatatt
    15541 gcgattttcc agcggcagat ttgcctgcgc ccgcgtctgc tccgcctcct ggcgggcgat
    15601 attgtcattc tgccaggcaa cggaatgcac cgagagaatg ttgccctcct ggcgcgttat
    15661 caatccggcg cttttcacta cagtttgtag tgcctgcttc cagggaacgt cagttaaatg
    15721 taacgacacc gtaccgctga cgtctggcga aaccaccagg ttcaacttct cctgttcagc
    15781 cagcgcctgc aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg
    15841 ctttgccgcc tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat
    15901 ttgtatctcc ttgccgttgc cacaaccagt gtggcggttc gcagtttgtc ccggttccca
    15961 gcgttagtgc gtctggcgtc agctgtaaga tcgtccagcc gttttccagc acatcgtttt
    16021 gctgcacccg tcgccatttt ttttgcccat cttttattac accgatgatg cgctcgcctc
    16081 gccctaccat cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg
    16141 gcggtttaaa agggtcacgc ataccggtta aaaggcacaa tgcaatacct gccaacaacc
    16201 agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga acaaaaggtc atcaccttcc
    16261 acgcttaacg aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac
    16321 ggcactgctt cccacagcgt tttcaacgcc aactcaccac cctgcgcgga tggatgccag
    16381 gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttct
    16441 tcgctgaagg gagtggcgtc taccaggcga tacagtgcag cccactgctg gtgatgactc
    16501 gcccgtagtc gaattaatgc gtcgcgctct tcctggtgtg tcgatgacag aaaaatgagc
    16561 gtaactaaca ccagcaacca gactgcccag caaagctggc ggaggcggag tgatgtggcg
    16621 aaccaccagt caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg
    16681 ccagcgtccc tgcgcatcct gctgcgtggc cccccgctga ttgagatgaa aagaagcatc
    16741 ctggcggagt gacgtttcca gcgcgtttaa cgcggtaatg cttgttgtaa gccccttgat
    16801 ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag gcatgttcgg gtaaaagcgc
    16861 cgctagtgct tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgacgttg
    16921 caaacgctgt tcgcgtaact gctggcgttc cagcaaacgt ggtttcgtta tctgtaagct
    16981 gcgggtgagt tgttgttccg cctgaagcaa aacggcgttt acgcgtgctt cggcattgct
    17041 tgtcagacgc agtattagcg ttatcccgac ggccagcagc aaaggcgcaa cgaacatcag
    17101 caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg
    17161 cgggttcatc agtacacctt cccaagcgcc agccccagcg cgatggcaaa gtctccacct
    17221 ggtggcggta gcggcggctg acgaacagaa accgcctccc aggggtcaaa tccgccttcg
    17281 ccacatagcg cgacgctttc gggatcaaca gataacgctg ccgccagctc cttcgcgcta
    17341 gtcatcccca ccgccagttt gcgcccccag ctatagcgcg tcgcccacag ccactgttcg
    17401 ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat
    17461 gcactggcat ccggagtgat cgcgctaaca tgaacacgca acctttctgc cagagtaagc
    17521 agcgttgcca gctctttgct ttgcgcggca gtcacgttat aggcgggact gagtgagtct
    17581 tcgctgtaat cgaagcgcag ggagtccgga tccatatcca gttcacgggc catcgtcccg
    17641 gacagccagg ccatttgctc ccgctcaccg agggacatcg acgggcgtgg aaatgaccgc
    17701 tgtaatgtgc gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga
    17761 ctccacggta acaacgtgtt agccagccgc tgcacatcaa caatccgccc atctttgata
    17821 atgtcgttct ccagtggcaa ccgccaccag cggtgcaata agcattcttt tgcgctccgc
    17881 acgatagcaa ccgctaccgc ttcttgctgt tgtaaatgca aaccaatttg ccagttctta
    17941 aatgccattg tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg
    18001 gcttgccttt atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa
    18061 atttccagtg aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg
    18121 agcaggctcg atttatggcc tataccgcta catcgagcca caactgccgg atgtggcgac
    18181 attaaaagat gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat
    18241 tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc cgccggagat
    18301 ggtgaaagcc tttatcgcga cggaagacag ccgcttctac gagcatcacg gcgttgaccc
    18361 ggtggggatc ttccgtgcgg caagcgtggc gctgttctcc ggtcacgcgt cacaaggggc
    18421 gagtaccatt acccagcagc tggcgagaaa cttcttcctc agtccagaac gcacgctgat
    18481 gcgcaagatt aaggaagtct tcctcgcgat tcgcattgaa cagttgctaa cgaaagacga
    18541 gatcctcgag ctttatctga acaagattta cctcggttac cgcgcctatg gtgtcggtgc
    18601 tgcggcacaa gtctatttcg gtaaaactgt cgaccaactg acgttgaacg aaatggcggt
    18661 gatagccggg ctgccgaaag cgccttccac cttcaacccg ctctactcaa tggatcgtgc
    18721 cgtcgcgcgg cgtaacgtcg tgctgtcgcg gatgctggat gaagggtata tcacccaaca
    18781 acagttcgat cagacacgca ctgaggcgat taacgctaac tatcacgcgc cggagattgc
    18841 tttctctgcg ccatacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga
    18901 aagtgcctat gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc
    18961 cgcacagcag gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg
    19021 cccggcaaat gtgctgtgga aagtgggcga atcggcgtgg gataacaaca agattaccga
    19081 tacgctgaag gcgctgccga cctatggtcc gctgctgcct gccgcagtca ccagcgccaa
    19141 tcctcaggaa gcaacggcga tgctggcaga cgggtcgacc gtcgtattga gtatggaagg
    19201 tgttcgctgg gcgcgtcctt accgttcgga tactcagcag ggaccgacgc cgcgtaaagt
    19261 gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt caagttgacg atgcatggtg
    19321 gctggcgcaa gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc
    19381 cgttatggcg ctggtcggtg gctttgattt caatcagagc aagtttaacc gagccactca
    19441 ggcactgcgt caggtgggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa
    19501 aggtctgacg ctggcaagta tgttgaacga tgtgccaatt tcccgctggg atgcaggtgc
    19561 cggttctgac tggcagccga agaactcacc accgcaatat gccggtccaa ttcgcttacg
    19621 tcaggggctg ggtcagtcga aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt
    19681 cgactacgct gcagaatatc tgcaacgctt cggcttcccg gcacaaaaca ttgtccacac
    19741 cgaatcgctg gcgctgggtt cagcttcctt caccccaatg caggtggcgc gcggctacgc
    19801 ggtcatggcg aacggcggct tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga
    19861 tcagggcggc gtaattttcg aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc
    19921 ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt
    19981 cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa
    20041 tcaggcgtta gtggcgaaga ccggcgcgca ggagtacgca ccgcacgtca tcaacactcc
    20101 gctggcattc ctgattaaga gtgccttgaa caccaatatt tttggtgaac caggctggca
    20161 gggtactggc tggcgagcag gtcgtgattt acaacggcat gatataggcg gaaaaacggg
    20221 taccactaac agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc
    20281 ggtctggatt ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc
    20341 gattaaagat cagatctcag gttacgaagg cggtgccaag agtgcccagc ctgcatggga
    20401 cgcttatatg aaagccgttc ttgaaggtgt accggaacag ccgctgacgc cgccaccggg
    20461 tattgtgacg gtgaatatcg atcgcagcac cgggcaatta gctaatggtg gcaacagccg
    20521 cgaagagtat ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg aggtgggaac
    20581 gaccattatc gataatggcg aggcacagga attgttctga ttaaaaaggc gcttcggcgc
    20641 ctttttcagt ttgctgacaa agtgcacttg tttatgccgg atgcgggtga acgcgttatc
    20701 cggccaacaa aaaattgaaa attcaataaa ttgcaggaac ttcgtaagcc tgataagcat
    20761 tatgcatcag gtaaacttca cgcatttaca ctcgcccctg ccctttcaac cattcgcgca
    20821 cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc aggggcttcc agcaaatcca
    20881 tcatatgcgc cagcggccag cgcacctgtg gtaacggctc tggctcatcg ccttccagtg
    20941 attccgggta gagatcttgc gctaccacga tattcatttt gctggaaaag taagacggtg
    21001 ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc aaatccaacc tcttctttta
    21061 gctcgcggtt agcggcttca aagacgcttt cacccggatc aattaatcct ttcgaaaaac
    21121 ctaattcgta ggattcagtt cccactgcgt attcgcgaat caggatcagg tgatcgtcca
    21181 caatcggcac aatcatcact gcttcccggt tggttggacg cattcgttca taaacacgcc
    21241 gcacgccatt gctgaactcc agatccacgc tttcgacggt aaacagtcgg gaacgggcta
    21301 cagtttcaac attcagaatg gtgggttttt gtaatgattt gctcatcgtg ggatctatgc
    21361 tgtgaaatca gcggttattg tgcgatatcg gacacgcttt cggcaatgtg aattgcgcgt
    21421 tatttacatt tatgtaactt aataaataat cgtcctcaaa tcaaattaaa agtcaatagg
    21481 ttgaaataac tccaggaatt tgctgatatt ccgccttcag agggtttgct atgatcagcg
    21541 gttactgtga tgtgcttaat gatgctcaag ttaaactcca cgcttgccga tagccaaccg
    21601 cagaatcatg tattgtgtcc ggtgcgactg accacgcctg acagactaag taagatgggg
    21661 aaagcatgag caccattgtg atttttttag ctgctttgct ggcctgctca ctacttgcgg
    21721 gatggctgat aaaagtgcga tccagacggc gtcagctgcc ctggaccaac gccttcgcgg
    21781 atgcgcaaac gcgtaaactc acatctgaag aacgtagcgc cgttgaaaat tatcttgaga
    21841 gcctgacgca ggtattacag gtgcctggcc caacgggagc cagcgcggca ccgatctctc
    21901 tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac acacgctatc acgcgttatg
    21961 gcatctctac cgacgatccg aataaatggc gttactacct cgattcggta gaagtccacc
    22021 tgcccccttt ctgggaacag tacatcaacg atgagaatac cgttgaactg attcataccg
    22081 attcgctgcc gctggttatt tcactcaacg gtcatacgct gcaggagtac atgcaggaaa
    22141 ttcgcggcta tgccttgcaa cctgttccgt caacgcaggc gtcgattcgc ggggaagaga
    22201 gcgagcaaat agagctgctc aatattcgca aagaaacgca tgaagaatat gcgctgagtc
    22261 gtccgcgcgg gctgcgcgaa gcgttgctga ttgtcgcctc cttcctgatg ctctttttct
    22321 gcctgattac cccggatgtc tttgttccgt ggctggcagg cggcgcgtta ctgctgctgg
    22381 gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc ctccctgcgg gaaattcatt
    22441 gtctgcgcgg tacaccccgt cgttggggat tgtttggcga aaacgatcag gaacagatca
    22501 acaatatttc actcggtatt atcgacctgg tctatcccgc acactggcag ccctacattg
    22561 ctcaggatct cggccaacaa accgatatcg atatctatct cgaccgccac gtagtgcgtc
    22621 aggggcgata tctttcgctc catgatgaag taaaaaactt tccgttacag cactggctgc
    22681 gcagtacgat tatcgctgcg ggctcgctac tggtgctgtt tatgctgtta ttctggatcc
    22741 cgctggatat gccgctgaaa ttcaccctct cgtggatgaa aggcgcgcag accattgaag
    22801 ccaccagcgt gaaacaactg gctgacgctg gcgtgcgggt tggcgataca ttgcgtatta
    22861 gcggtacggg aatgtgtaat attcgcactt ccggtacctg gagcgcgaaa accaactcac
    22921 cttttttacc gtttgactgc tcgcagatca tctggaacga cgcccgctca ttgccattac
    22981 cagaatctga attggtcaac aaagcgacgg cattgaccga agcagttaat cgccagctgc
    23041 accctaaacc ggaagatgaa tctcgcgtca gcgcctcatt acgttcagca attcaaaaat
    23101 ccggcatggt attgcttgat gattttggcg acatagtact gaagacagcg gatctgtgtt
    23161 ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt caatctcggc aacagtaaag
    23221 actgggacgc gctggtaaaa cgcgccaacg ccgggaaact cgatggcgtg aatgtgttat
    23281 tacgcccggt gagtgcggaa tcgctggata acctggtggc cacctctacc gcgccgttta
    23341 tcacgcatga aacggcgcga gcggcacaat cactaaacag tccggccccc ggcgggttcc
    23401 tgattgtcag cgacgaaggc agcgattttg ttgatcagcc ctggccttcg gcatcacttt
    23461 acgactatcc gccgcaagaa cagtggaacg ctttccagaa actggcacaa atgctgatgc
    23521 atacgccgtt taacgccgaa ggtatcgtca caaaaatctt cactgacgcc aatggtacgc
    23581 agcatattgg ccttcatccg atcccggatc gttccggtct gtggcgctat ctcagcacca
    23641 cgctgctgct actgacgatg ctgggtagcg ccatttataa tggcgtacag gcctggcgtc
    23701 gttaccagcg tcatcgtact cgcatgatga agattcaggc ctattatgaa agctgcctga
    23761 acccgcaact gatcacccct tccgaaagcc ttatcgaata acacgtttgc gcggcaggtt
    23821 atgctaccct gtcgcgcaaa ttgcttcact ctggagattt ccctcatgca tatcaacatt
    23881 gcctggcagg acgtagatac cgttctgctg gatatggacg gcacgttgct cgacctcgcc
    23941 ttcgataact atttctggca aaagctggtg cctgaaacat ggggcgcgaa aaacggagtt
    24001 acgccacagg aagcgatgga atatatgcgc cagcaatatc acgacgtaca gcatacgcta
    24061 aactggtact gtcttgatta ctggagtgag caactgggtc tggatatctg tgcgatgacc
    24121 accgagatgg gaccgcgtgc cgtactgcgt gaagatacca ttccgtttct tgaggcactg
    24181 aaagccagcg gtaaacagcg aattttgctc accaatgcgc atccgcacaa cctggcggta
    24241 aaacttgagc ataccggtct ggacgcacac cttgatttat tactttccac ccacacattt
    24301 ggttatccga aagaggatca gcggttatgg catgcggtgg ccgaagctac gggtcttaaa
    24361 gctgaaagaa cgctgtttat tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt
    24421 ggtattcgtt actgcctcgg tgtgactaat cctgactccg ggattgccga gaaacagtat
    24481 cagcgccatc cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga
    24541 cgccatgaaa gaaaaacctg ctgttgaggt tcgactggat aaatggctat gggccgcccg
    24601 tttttataaa acccgcgcgc tggcccgcga aatgatcgaa ggtggtaagg tgcattacaa
    24661 cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca
    24721 gggaaatgac gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc gccccgccag
    24781 cgaggcagtc ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc
    24841 gctggcacgt aaacttaatg ccttaaccat gccgcatccg gaccgacgcc cggacaaaaa
    24901 agagcgccgc gacctgttac gatttaaaca cggcgacagt gaataactgt cacctgcaag
    24961 agagatgatt atgccgcaac atgaccaatt acatcgctat ctgtttgaaa actttgccgt
    25021 tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag atccttgaga atcacgatta
    25081 tccgcagccc gttaaaaacg tgctggcaga actgctggtt gcgaccagcc tgttaaccgc
    25141 tacgttgaag tttgatggtg atatcaccgt acagctgcaa ggcgacggtc cgatgaatct
    25201 ggcggttatt aacggtaaca acaaccagca gatgcgcggt gtggcgcgcg tgcagggcga
    25261 aattccagaa aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat
    25321 caccccgagc gaaggcgaac gctatcaggg cgtggttggt ctggaaggtg ataccctggc
    25381 ggcctgcctg gaagattact ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg
    25441 caccggcgac gtagacggca aaccggctgc aggcggtatg ttgttgcagg taatgcctgc
    25501 gcaaaatgcc cagcaggacg actttgacca cctggcgacg ctaaccgaaa ccatcaaaac
    25561 cgaagaactg ctgactttac cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga
    25621 ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg
    25681 cgccgatgcg ctgaaaacgc tgcctgatga agaagtcgat agcatcctgg cggaagatgg
    25741 cgaaattgac atgcattgtg attactgcgg taaccactat ctgttcaatg cgatggatat
    25801 tgccgagatc cgcaacaacg cgtctccggc agatccgcaa gttcattaat gagtatgtcc
    25861 ggcagagagg actctgccgg attttacgac gtaatggcct gatgcgctgc gcttaccagg
    25921 cctatgaatt gacatcctcc acgccctgaa ggacgtggat ccctgctacg ttcaggctgt
    25981 cgcctgaatc atttcggtgg gttcctgctt caacgggcgg cctgactgca ccatctctcc
    26041 acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccattta catcggcgtt
    26101 cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcggt tttctttcgc
    26161 tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag caagcacctg
    26221 accgccactc cagagctgct tgtgctcaag ctggcggcgc atttcatacc agccctgatc
    26281 cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt
    26341 acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattgcgtg
    26401 gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca
    26461 ggaatgcagc cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt
    26521 gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg tttttctgaa aactgtttac
    26581 aggctcaaag actgtgccat ctgacagcgt ggcgagttta gccacgccag catccagccc
    26641 gaccattgat gctgaagggt gaaccggagt tgatacttca ctttctgtct gaatactgat
    26701 gtaccactta ccgcaggact ggctgacagt gacatttttc acaactcccg tgacctgacg
    26761 gctattccgg tagcgcatcc aaccaagttt cggcagaaaa atacggctgt tttcctgatc
    26821 gagcttaacg ccctgcgggt agcggaatgc atcattctgt ccccgctttt tgaatcgggg
    26881 aaaagcagcc cgcttctgga agaagttttt gtaagcccgc tcaaggtctt tcagtgactg
    26941 ttgcaatggc tgtgagggag aatctttaag ccattgcgtt tcagtggcat ttttccactc
    27001 aaccagccag gaagccattt tcccgtaagg gatgtattta ttaccggcct catgattctc
    27061 attctgaagc gccagagcac gattgaaaac gaaacgacat gcgcctgcga agcgcctcat
    27121 ttcacgctcc tgttgaccac cgggtcttaa ctggaattta aatgcctgta gtcgtttcac
    27181 aggtcaaatt atattttggt ctatgaaaaa agaaaccgat atccggcgtg gaaggcattg
    27241 tgttttcctg atgcatgttc acctggtctt tgtcgccaaa taccaacgcc agatttttga
    27301 ccatgacgcg acagaaaaac tacgcactta cttttcaaat gtatgtgctg attttgaagc
    27361 tgaacgggtt gaaatggatg gcgaacttat atccccgccc tgaaggacgg ggttttaccg
    27421 cgcaccggat aaatcgcaag ttattttggt cagatacagc gtcgtatccg ttaaaagcgt
    27481 tgtcacttac ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc gcgccacgct
    27541 ttcggcctcg tccccagttt tccatccttc gcagctgtga gtaatttttc tttcgttatt
    27601 ttgcgcggaa gtaaactcat atcaagcgcg tcccattctt ttctggctga gtcaaagcca
    27661 tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg accatcacta
    27721 ttcagatctt gctcgaccag tacgcaggcg tctttttcaa tacaggtcat cacgttatag
    27781 cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taccagaacc tggggcaatt
    27841 aacacattat cggctaatac tttttccgat acctgttcct gaagatgttg ctctccatct
    27901 aacgccatca gcagatcccg cgcgcgtttc gggtctttca tatactcggc atcgcttttc
    27961 agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta gatagttacc
    28021 tggtctggcg tgtttttacc gctctgataa cgcgccatat gactgttcac gctaatgcgc
    28081 atactgtcca gcaccggcga attaagaagc accagtatta ccaacaccaa taacgaaacc
    28141 gcaaggttca ctttgccctg aagatcaagg ggattttgcc ctttacgcca gacgatactg
    28201 acaaaatacc ccagcgacca taccagtaac accagcaccg ccagcgcgcc ctgcaagcgg
    28261 tcgacagtcc agccgtactg agcgacccgc agccataacg cccaggcggc gacgaacaca
    28321 tacagcggag caaccagcaa agcggtttta atcaggcaac gcaatggccc cgtccaggga
    28381 agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa aaaggccagc
    28441 gtcaacagca acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc cgtaaacggc
    28501 agggtgataa taaacatcag ggttagcaat gatactaacg gcagcaaccc cgtggcgatt
    28561 aacgtgaaca acttttgaat agagtcgatt aaacgtgact gtgttcgcgc gaggatcacc
    28621 gccagcgcgg taaccagacc taacgtgaga taaataaacc agtcggttgc aaaaaagagc
    28681 gttttgaaaa acgtgatgcc aacaagttta aacaactcac tccacagtaa aagtaccagc
    28741 cacgttaggc cattggcgag aaaaatcacc agtaatataa gtacgttatg ccatactgac
    28801 tggtaaaagt agcgatagcg gctactgtca ttgcggatgc gaaggctttg ttgtatccac
    28861 ggtagcaaca acattgccat cagcagcaga tagcagccaa aatcccaaag cgccttttca
    28921 gctctccagg gggtcatgcc atcagtttgc cacttcagca agccgctcat tcccagcgtg
    28981 gcaataaaca ccagcgccag ccatccccaa aggcgattct gtttaaagga gatcacggag
    29041 aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat ccaactgaga
    29101 ttttttccgg caagccaggc gatcagcagg tagcaaacca gcccctgaag taatccggta
    29161 gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt ggttatccag
    29221 aatcaaaagg tgggttaatt atcgcatcgg gcagtagtat tttgcttttt tcagaaaata
    29281 atcaaaaaaa gttagcgtgg tgaatcgata ctttaccgat tgaatttgca tcaatttcat
    29341 tcatgaatgc ggttccactc acaatattcc cgccatataa accaagattt aaccttttga
    29401 gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga
    29461 atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc
    29521 gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag
    29581 tgaaatgcgc gttaacaatg gtttgacccc gcaagaactc gaggcttatg gtatcagtga
    29641 cgtacatgat atcgtttaca acccaagcta cgatctgctg tatcaggaag agctggaccc
    29701 gagcctgacc ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac
    29761 cgggatcttc accggtcgtt caccgaaaga taagtatatc gttcgtgacg ataccactcg
    29821 cgatactttc tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc
    29881 ggaaacctgg cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt
    29941 cgttgtcgac gccttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac
    30001 cgaagtggcc tagcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga
    30061 gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca
    30121 gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat
    30181 gcagctgatt ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat
    30241 gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga
    30301 gaaaggcgat gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac
    30361 cgacccgaaa cgccgcctga ttggcgatga cgagcacggc tgggatgatg acggtgtgtt
    30421 taacttcgaa ggcggctgct acgcgaaaac tatcaagctg tcgaaagaag cggaacctga
    30481 aatctacaac gctatccgtc gcgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg
    30541 cacaatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccgatcta
    30601 tcacatcgat aacatcgtta agccggtttc caaagcgggc cacgcaacta aagttatttt
    30661 cctgacagca gacgcctttg gtgtgctgcc gccggtttct cgtctgactg ccgatcaaac
    30721 ccagtaccac ttcctctctg gcttcaccgc caaactggct ggtacagagc gcggcatcac
    30781 cgaaccgacg ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac
    30841 ccaatacgca gaagtgctgg tgaaacgtat gcaggcagcg ggtgcgcagg cgtatctggt
    30901 taacaccggc tggaacggca ctggcaaacg tatctccatt aaagataccc gcgctattat
    30961 cgacgccatc ctcaacggtt cgctggataa cgcagaaacc ttcactctgc cgatgtttaa
    31021 cctggcgatc ccaaccgaac tgccgggcgt agacacgaag atcctcgatc cgcgtaacac
    31081 ctacgcttct ccggaacagt ggcaggaaaa agccgaaacg ctggcgaaac tgtttatcga
    31141 caacttcgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa
    31201 gctgtaatga tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga
    31261 ggcaccttcg cctcccgttt tatttaccct tcttttgtcg tgccctgcgc ccgcgttacc
    31321 ggcactggca gccaggcgcg aatggaaagc ccgccccgtt cgctggtgcc aagctccagc
    31381 atcccgttat ggttatccac gatacgctgc acaatcgcca gccctaatcc cgtgccgcta
    31441 atagtgcgcg cactgtcgcc acgaacaaac ggctggaaca ggtgcttacg ttgttccggc
    31501 gcaatacccg gaccgtcatc ttctacctgg aaccaggcgc gattcggctc cgtgccgctg
    31561 ctgactttga tccagccatt gccgtaacgg gcggcgttga ccaccatatt cgctaccgcg
    31621 cgtttgatcg acagcgggtg cattttcacc tcaatgctgc cggggtaaag cgcagtttca
    31681 atttcccgct catagccact ttcggcagca ataacctcac cgagtactgc gttgagatcc
    31741 gccatttcca tcggcatctc ctgcccggtg cgcaggtagt cgataaactg ctcaatgatg
    31801 gcgttgcact cttcgatatc tttattgatc gattctgcca ggtagccatc ctgctcgctc
    31861 atcatctcgg ttgccaggcg aatacgcgtc agcggcgtgc gcagatcatg gcttaccccc
    31921 gccatcagca gcgtgcggtc atccgccagt tgcttaacac cagccgccat atgattaaag
    31981 gcgcgggtaa cggaacgcac ctccgaagcg ccatattcac gcagcggcgg cggaataatc
    32041 cctttaccca cctgcaaggc tgcgtgttcg agatcgacca acggtcggtt ctggatacga
    32101 ataaacagcc acgccccgcc tatcgccaat agcataatcg ccaacgtata acggaacagc
    32161 ggggagaaat cgccctgatg aatttcggtt agcggcacgc gtacccagat attgggagac
    32221 aaccaggttt tcagccagac gacaggcgaa cttttgttaa cctcgacgcg aacttccgtc
    32281 ggaccgccca gttgctgcgc catctgatgg cttaagaatt catagtgttg cgcccaacgc
    32341 agcccggcct cttcggcggc ttcgttggaa tagagagaga tccccagctc acggtagatc
    32401 tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt cctccagttg cagtttgtcg
    32461 gtcatcaaca tacgcacttc gtacgcgagg actttattaa actgctggag gctcggcaaa
    32521 atcgcgaagt tcagcaccac cagataagtc gtcaccaggc tgacaaacag caaggtgacg
    32581 atgagcaata acgtacgggc aaatgaactt cgtggcgaga agcgcaatcg cctcatgctt
    32641 tagaaccgtc cggtacaaag acgtagccca gaccccagac ggtctgaatg taacgcggat
    32701 gcgccggatc ttcttccacc atgcggcgca gacgcgaaat ctgcacgtcg atggagcgtt
    32761 ccattgcgga atattcacga ccacgggcaa ggttcatcag cttatcgcgg gagagcggct
    32821 cacgcggatg gctgaccagt gccttcagta ccgcaaactc accactggtg agcggcatcg
    32881 gctcgtcttc gcggaacatt tcgcgcgtac cgaggttaag tttgaactta ccgaaagcaa
    32941 ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt cgcctgacga cgcagcaccg
    33001 cacgaatgcg ggccagcagt tcacgcgggt taaacggttt tggaatgtaa tcgtcagcgc
    33061 caatctccag gccaacgata cggtccactt cttccccttt cgccgtcacc ataatgatcg
    33121 gcatcgggtt actctggcta cgaagacgtc ggcaaatcga caagccatct tcaccaggta
    33181 acatcaaatc cagtaccata agatggaaag attcacgagt cagcaggcga tccatctgtt
    33241 ctgcattagc gacgcttcga acctggaagc cttgttcggt gagataacgt tccagcagcg
    33301 cgcgcaggcg catgtcgtca tcaaccacca gaatcttgta gttctcttgc attgtttgta
    33361 ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca cgttcgtcac
    33421 cagctaaatc tggtatgaat ttcagcctaa attgttacaa agcatattaa acagcagctt
    33481 aagtatacaa tttattcggc gaaacattat tgattctgtt gatatgatca cgttataccc
    33541 aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc cctggttacc
    33601 cgggaagggt atgaaaaact caaacaagag cttaattatc tctggcgtga agaacgcccg
    33661 gaggtcacaa agaaagtgac ctgggccgca agtctgggcg accgcagcga aaatgctgac
    33721 tatcagtata ataaaaagcg cctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa
    33781 tgcatggaaa atctcaaaat cgtcgattac tccccgcagc aggaaggcaa agtctttttt
    33841 ggtgcgtggg tggagattga aaacgacgat ggtgtgactc accgtttccg tattgtcggc
    33901 tacgatgaaa tttttggccg taaagattac atctctatcg attccccgat ggcccgcgca
    33961 ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata cccctgccgg ggaagcgagc
    34021 tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac ctcctgccga
    34081 tggctggcat ttttgccagc cagtccgtat aactatcccc tgatttttga tccgaaaaga
    34141 tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta ttgcgggtga aattcaggcg
    34201 cgcccggaac aggttgacgc tgccgttcgc ctgcttgacg aagggaatac cgtgccgttt
    34261 atcgcacgtt atcgtaagga aatcaccggc ggtctggatg acacgcagct gcgtaatctg
    34321 gaaacgcgtc tgagctatct gcgcgagctg gaagagagac gtcaggcgat cctcaagtct
    34381 atttccgagc aaggcaaact caccgatgat ctggcgaatg ccatcaacgc caccctaagc
    34441 aaaaccgaac tcgaagacct ctacctgccc tacaaaccta aacgccgcac ccgtgggcaa
    34501 atcgccattg aagcagggct tgagccgttg gccgatctgc tgtggagcga tccgtcacac
    34561 acgccagaag tcgccgctgc acaatatatt gatgccgata aaggcgtggc agataccaaa
    34621 gccgcgctgg acggcgcgcg ctatatcctg atggaacggt ttgccgaaga tgccgcgctg
    34681 ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatc tggtctcaac cgtggtgagc
    34741 ggtaaagaag aggaaggggc aaaattccgc gactatttcg atcatcacga accactgtca
    34801 acggtgcctt ctcaccgcgc actggcgatg ttccgtggtc gtaacgaagg catacttcag
    34861 ctttcgctga atgccgatcc gcagttcgaa gagccgccga aagagagcta ttgcgagcaa
    34921 atcatcatgg aacaccttgg cctgcgcctg aacaatgccc cggcggatag ctggcgcaaa
    34981 ggtgtggtga gctggacgtg gcgcatcaag gtgttgatgc atctggaaac tgaactgatg
    35041 ggcaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg tctttgcccg taacctgcac
    35101 gatctgctga tggcggcccc tgccggactg cgtgcaacga tgggcctcga tccgggtctg
    35161 cgtaccgggg taaaagtggc ggtagtcgat gcaactggta aactggtggc gaccgatacc
    35221 atttatccgc ataccgggca ggccgcaaaa gcagcgatga ccgttgctgc gctgtgtgaa
    35281 aagcataacg ttgaactggt ggcgatcggc aacggtacag cttcccgcga aaccgaacgt
    35341 ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg cgcagaaagt gatcgtcagc
    35401 gaagctggcg cgtcggttta ctcggcttcc gagctggcag cacaggaatt cccggatctc
    35461 gacgtttcgc tgcgtggcgc ggtgtctatc gcccgtcgtt tgcaggatcc gctggcggag
    35521 ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt atcagcatga cgtcagccag
    35581 acgcagctgg cacgcaaact ggatgcagta gtagaagact gcgtaaacgc cgttggcgtc
    35641 gatctcaaca ccgcttctgt tcctctgtta acccgcgtgg cgggcctgac gcgcatgatg
    35701 gcgcaaaaca tcgtagcctg gcgcgatgag aacggtcagt tccagaaccg tcagcaactg
    35761 ttaaaagtga gccgtctggg gccgaaagcc ttcgagcagt gcgcgggctt cttgcgcatt
    35821 aaccacggtg ataacccact ggacgcttct accgttcacc cggaagccta tccggtggtg
    35881 gaacgcattc tggcagcaac acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa
    35941 ctgcgtaacc tgaaagcgtc tgactttact gatgagaaat tcggtgtgcc gacggtaacc
    36001 gacatcatca aagagctgga aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct
    36061 cagtttgccg atggtgtcga gacaatgaac gacctgcaac cgggtatgat cctcgaaggt
    36121 gcggtgacca acgtcaccaa ctttggcgcg tttgtcgata ttggcgtgca tcaggacggc
    36181 ctggttcaca tctcttcatt gtcgaacaag tttgtggaag atccgcatac cgtggtgaaa
    36241 gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc
    36301 ctgaccatgc gtctggacga gcagcctggc gaaaccaacg cccgtcgcgg cggcggtaat
    36361 gaacgcccgc aaaacaaccg cccggcagcc aaaccgcgtg ggcgtgaagt gcagcctgcc
    36421 ggtaacagcg cgatgatgga tgcgctggcg gcggcaatgg gcaaaaaacg ttaaacgccc
    36481 ggactggccc ctaaacctgc gcgattaggc gttcacgcca catctggcaa cgaacgcctt
    36541 gcctgacata aaagtgccgg agaatatctc cggccttttt attccacaag caaacgcata
    36601 atatatcccg ttaatattta tcacttcatt aacaactgaa accttaatta aacattaacc
    36661 agccctaata attcgtcatg cgaattatat tttcgctgcg atataagctt gagccatatc
    36721 aacattgagt cagattatta ttcaaaccaa cattcacaca cattttaagt attgctgata
    36781 gaaaccattc tcattatcat tgtactgttg attatttaat ctctccttcg ttggcaaatc
    36841 atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta atgagaaaca
    36901 agtaggcacc tatgcaatac actccagata ctgcgtggaa aattactggc ttttcccgtg
    36961 aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct ggctcctctt
    37021 ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc cgtcgtgtga
    37081 gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt tcctgttaat
    37141 acggtgataa caacaatgaa aaaattaacc attggcttaa ttggtaatcc aaattctggc
    37201 aagacaacgt tatttaacca gcttactggc tcacgtcagc gtgtaggtaa ctgggctggc
    37261 gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt cacgctggtg
    37321 gacctgcccg gcacctattc tctgaccacc atctcatcgc agacctcgct cgatgagcaa
    37381 atcgcctgtc actacatttt gagtggcgac gcagacctgc tgattaacgt ggtggatgcg
    37441 tctaaccttg agcgtaacct gtacctgacg ctgcaactgt tggaactcgg cattccctgc
    37501 atcgtggcgc tgaacatgct cgacattgcc gagaagcaaa atattcgtat tgaaattgat
    37561 gctctgtcgg cgcgtcttgg ctgcccggtg atcccgctgg tttcaacccg tggtcgcggt
    37621 attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt ggaactggtg
    37681 cattacgctc agccgctgct caacgaagca gattcactgg caaaagtgat gccttccgac
    37741 atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga tatctacagc
    37801 cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg tctgcgtaat
    37861 gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat tgctgccatc
    37921 tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac tgcggtagat
    37981 aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat gtacctgatg
    38041 ttcttgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt cggctccgta
    38101 gcactctttg tgcatggtat tcagtggatt ggctacacgc tccacttccc ggactggctg
    38161 actatcttcc tcgcccaagg cctgggcggc ggtattaaca ccgtgctgcc actggtgccg
    38221 cagatcggca tgatgtacct gttcctctcc ttccttgagg actccggcta tatggcgcgt
    38281 gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa atcctttgta
    38341 ccgctgatcg ttggtttcgg ttgtaacgta ccgtcggtaa tgggcgcacg tacgcttgat
    38401 gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt
    38461 ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc gctggcggtc
    38521 ttctcgctgt atatgctggg tatcgtaatg gcggtgctga ctggcctgat gctcaagtac
    38581 accatcatgc gcggtgaagc gacgccgttt gtcatggagc tgccggtcta ccatgtaccc
    38641 cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt tctgcgtgct
    38701 ggtaaagtga ttatcatcgt cagcattttc ctgagcgcct tcaacagctt ctcattgagc
    38761 gggaaaatcg tcgataacat caacgactcg gcgctggctt ccgtcagccg ggtgatcacc
    38821 ccggtcttca agccgattgg cgtacatgaa gataactggc aggcaacggt tggcctgttt
    38881 acaggtgcca tggcgaaaga agtggtggtg ggtacgctca acaccctcta caccgcagaa
    38941 aatattcagg acgaagagtt caatccggcg gaatttaacc tcggtgaaga gctgttcagt
    39001 gcggtagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt actgatgaac
    39061 cccattgaag caagcaaagg cgacggcgaa atgggtaccg gagcgatggg cgtgatggat
    39121 cagaaattcg gtagcgcagc tgccgcatac agctacctga ttttcgtcct gctgtatgta
    39181 ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc
    39241 tccattctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta tcagatcgcc
    39301 agctacagcc agcatccaac ctacagcctg gtatgcattc tggcggttat tctgtttaac
    39361 atcgtggtta tcggtttgct gcgtcgcgcg cgtagccggg tggatatcga actgctggca
    39421 acgcgcaaat cggtaagcag ttgctgtgcg gccagtacca ctggcgattg ccactaagga
    39481 cacgacaatg gcttcgctca ttcaagtgcg tgatttactg gcattacggg gccgtatgga
    39541 agcggcccag attagccaga cactgaacac tccgcagcca atgattaacg ccatgctgaa
    39601 acagctagaa agtatgggaa aagcagtgcg gattcaggaa gaacctgacg gctgtctttc
    39661 cggcagttgt aaaagctgcc cggaaggaaa agcctgtctg cacgaatggt gggcgttgcg
    39721 ttaaccttgt ttcactgcct gttttgaaag cccggtatgc gtttgcatcc gggctttttt
    39781 gcgtgcggct ttccatagca gcgcgacgta agttcctctg aaagcgtctc gctgggatga
    39841 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aataacaggt
    39901 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactct
    39961 ttttacgcca accggagacg gcacgcgagc atggcgctgc tggagttaat tcaaaaacat
    40021 attcgtcaac gcgatctgat aggactggta gagcaaatgg cctgcttatt aagtagtgga
    40081 tacgcaaatg acagacaaat caaagggctg tttaattaca tactgcaaac cggcgacgcg
    40141 gtacgtttta acgattttat cgatggtgtg gccgaacgtt caccaaaaca caaggagagt
    40201 ttaatgacta ttgcggaaag attgcggcag gaaggggaac aatccaaagc cttgcatata
    40261 gcaaaaataa tgcttgaatc cggagtccct cttgcagaca tcatgcgctt taccggggtg
    40321 tcagaagaag agttggctgc ggcgagccag taaagttctg tcccgccatt tcgaaagcca
    40381 cctacaccct ctgcttcaac gccaccagca ggtgacaaaa ctcgtccgga tgtgaaataa
    40441 acggcgcatg ggccgctttg gcgaagatat atgattcgct gtgaggccaa agtttatcca
    40501 gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat cgcaaaaacg
    40561 gcatgggcac gttttgcagc ggctgacgga gatcgaccgt tttcaggatt tccagtccgc
    40621 cattgagtac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
    40681 catcctggcg cgccgtttca gtacccatgg tttgtaacgc caggaaccgc tccactgtac
    40741 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
    40801 gccactcgtc gcgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
    40861 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
    40921 aaatggcttt atctggtgcc tgtcgcagca cgacttcggc catatcagca agtgatagcg
    40981 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg
    41041 agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca
    41101 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
    41161 gttctctttt tacttacaag gatgaacata tgctaacagt accgggatta tgctggctat
    41221 gccgaatgcc gctggcgtta ggtcattggg ggatttgttc tgtctgctct cgcgccgccc
    41281 gtacagataa aacgttatgc ccacaatgtg gattacccgc cacacactct catcttccct
    41341 gcggtcgctg cctgcaaaaa ccgctgccct ggcaaagact gctcacggtc gccgactatg
    41401 cgccgccatt aagtccgctc attcaccagc ttaagttttc ccggcgcagt gaaatcgcca
    41461 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accggcttgc
    41521 aactgcctga tcgcatcatc agcgttccgt tatggcagcg ccgtcactgg cgtcggggat
    41581 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gctacactgc caatgggata
    41641 gcgaagccgt cacacgaaca cgggccaccg cgacccagca ttttcttaac gcccggctac
    41701 gcaaacacaa cctgaaaaat gcctttcgcc ttgaattgcc tgtgcaaggt cgccatatgg
    41761 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
    41821 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
    41881 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
    41941 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaaccagga agaagggaca
    42001 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg cgtttcttat
    42061 tgtccgccgg acgctgtgga agccaccgac actgccctga aatttgacct gctgaccgcg
    42121 tatgttgatg agttaagcgc tccttacctg gaagatgcag agatcgattt tgttaccgac
    42181 cagttgggtt cgcagctgac gctgaaagcg cctaacgcca aaatgcgtaa agtggctgac
    42241 gatgcgccgc tgatggagcg cgtggagtat atgctgcaat cacagatcaa cccacagctt
    42301 gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg aagacggtta cgccattttg
    42361 caatttggcg gtggctgtaa cggttgttcc atggtcgacg tcaccctgaa agaggggatc
    42421 gagaagcagt tgctgaacga attcccggag ctgaaaggtg tacgcgatct caccgaacac
    42481 cagcgcggcg agcactctta ctactaagtt ctctcccctt ttgtcgattg cccgatgtga
    42541 cgctaaagcg tcttatcggg cctgtacgtt gcacgagagt ctgacgttag cgcagaatta
    42601 tctcagcaaa tccaatctct tcgtttcccc acgataatat gaccaacctc tcataattta
    42661 aatttacccc gctctggtga ttctcaagca cgtaatgtta cccgtatcat tctcatgggc
    42721 accaaacaca ctcataacat ctgactacac taactcgttt taggggtcat cagccgtggt
    42781 gccgtaacat ctctgttccc agttgggaca accaggattt tccgtcagaa acctgacgtt
    42841 acccataaca aattaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta
    42901 atcttgttat tactcctgat gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc
    42961 gtggcgcttg ctgttggatt aatgcaaggg atgccgctgg ataaagttat tggctccatc
    43021 aaagctggtg tcggcggaac gctcggtagc cttgccctga tcatgggttt tggtgcgatg
    43081 ctgggcaaaa tgctggcaga ctgcggtggt gcacaacgta tcgctaccac gctgattgcc
    43141 aaatttggta aaaaacacat ccagtgggcg gtggtactga ccggttttac cgttggtttt
    43201 gccctgttct atgaagtggg tttcgtgctg atgctgccgc tggtgtttac catcgcggct
    43261 tccgcgaata ttccactgct gtatgttggt gtaccaatgg cggctgcact gtctgtgacc
    43321 cacggcttcc tgccaccgca tccgggtccg actgcgattg ccaccatttt caatgccgat
    43381 atgggtaaaa ctctgctgta cggtactatt ctggcaatcc cgaccgtgat tctcgccggt
    43441 ccggtttacg ctcgcgtgtt gaaaggtatc gataagccga tcccggaagg tctgtacagc
    43501 gcgaaaacct tcagcgaaga agagatgccg agctttggcg tcagcgtctg gacctctctg
    43561 gtgccggtgg ttctgatggc gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac
    43621 gctttcctgc cggtagcgga gttcctcggt gacccggtaa tggctacgct gattgccgtg
    43681 ctgatcgcga tgttcacctt tggtctgaac cgtggccgtt caatggatca gattaacgac
    43741 acgctggttt cttccatcaa aatcattgcg atgatgctgt tgattatcgg tggtggcggt
    43801 gcgttcaagc aggtgctggt agacagcggc gtggacaaat acattgcttc catgatgcac
    43861 gaaaccaaca tttctccgct gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg
    43921 ctgggttccg caaccgtcgc ggcaatcact gcgggtggta tcgcggcacc gctgattgca
    43981 acgacgggtg ttagcccgga gctgatggtt attgcggttg gttccggtag tgtgattttc
    44041 tctcacgtga acgatccggg cttctggctg ttcaaagagt actttaacct gactatcggc
    44101 gagaccatca aatcctggtc gatgctggaa accattatct cggtgtgcgg tctggtaggc
    44161 tgtctgctgc tgaatatggt gatttgataa tactgatggc cggatgcggc gtgaacgcct
    44221 tatccggcct acgattcggc attaattagt aggccggtaa gacgcgtcaa gcgtcgcatc
    44281 cggcatcaac cgcactctac ttcttcttcg ctgccgctct gcgccgtctg tccaaatcct
    44341 tcagcaactt gttcacacca tcatcggcaa acatcgactc aagcgttgcg gaaagtttac
    44401 gtcgccagtt tttatactgg taactggtgc cgggaatatt taccggatct gccatatcca
    44461 gccagtcttc cggctggagc cctaacaggg cgctgttgct atcggcgatg tagcgctgta
    44521 agccacgatt cagcgtcggt gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
    44581 gcaggcaacc atatttatgc agagcatcca gcagcccttg cttcgccagt tcgcgatcct
    44641 gatacagacc gcgcagtacc acttcatccg gatacagccc cagcgttttg cccagcgtta
    44701 aatccccact ctcccagtag ccgcgtagcg taggcaggtc gtgtgtcgcc gcaaccgcca
    44761 tcgactgctc tgggtacgct ttcggtgcgc ggaatgtctt ctcgtggtcg ttttcgaaat
    44821 agagcacttt gtaggagtac acaccgctgc tgcgcagctt accgacaatc tctaccggta
    44881 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
    44941 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
    45001 acggtatcca ccacaaacgc agcatcgaca tcacgtgatc aattcgtaat gcgccgcagt
    45061 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
    45121 gcgggtccat tggcggcaat ccccagttct gccccaacgg gccgaggata tccggcggcg
    45181 cgccaaccga cgctttcagg caatacagtt cacggtcgca ccaggtttct gccccacctt
    45241 ctgctacacc aacggccaga tcacgataca agccaatcgg catttcatag ccctggctta
    45301 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
    45361 cgtcatcacg atgttcttcg cagaactggc gcacttctgg cgaatccacg ttctgataca
    45421 tctctggcca tgctggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
    45481 gcgcatcaaa ggctgcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
    45541 acgcggtcat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
    45601 tcatttttag ggcggtaacc gtggagtaat caacccagtc ggcatcccgc gcctgttgca
    45661 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
    45721 cttcaactgc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
    45781 acgggctggc actttccgga tttgccagat agagcgcatg aatcgggttc agaccaataa
    45841 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
    45901 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
    45961 gtttattcag caacgcctgc ggttcgtaac agcgtttcgg ggcgacaatc acccggcaat
    46021 gcgcgcgctg gtcgtcctgg gtaagcgtca gcgtgtgata accttccggc agcttcgtcg
    46081 gtagattgaa cgctttgccc cccgtcacat ggcctttgta ctgctttcct tcttcggtgg
    46141 tcagcagcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
    46201 tataaactat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
    46261 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
    46321 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
    46381 tcgaacttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
    46441 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
    46501 ggtcgcggta cagcacatcc acctgctttt gtgcctccac ataagcggcg aaatctgcca
    46561 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
    46621 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
    46681 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaattgcc ttcacttgtt
    46741 ccacggtatg accaaaaata aagatatttt cttcgccgac tttctcagcg atttcaacgt
    46801 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
    46861 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
    46921 gtttttccgc cgccgaaaca caataatccg gcaagaacac caccttcaac ttgtcgccaa
    46981 ccagcggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atatttttcg
    47041 ccaggtagta gcccggtgct gctttcgcgc cgaagaggaa gacgcgcggt acgcggtcag
    47101 actgcgggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcagatgct
    47161 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
    47221 caataccggt acgaactttc acaaactctg ccagacggac tttattcgcc tgcttgatct
    47281 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagttgat
    47341 cgagatcgtt agcccactct tttttcagtg atttatccaa cagagccgcc agtgccgggt
    47401 tgcactgttt gacccagcga cgtggggtaa taccgttggt gacattatgg aatttgttcg
    47461 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
    47521 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttgt
    47581 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacta
    47641 gcgttttaaa gcgagtatta atttcgttaa taatctgcat gtggcgcggc agtaagcctt
    47701 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
    47761 cgaaagtttt gctggtaatg gcccaggcgt catcccagct catctggtgc tcatcgatca
    47821 gcacgcgcag cagttctgga atcgcgattg ttggatgggt atcgttcagc tgaataactt
    47881 cgtaatccgc cagttcgtgc aatttacgcc ccgccagatg atggcgacgc aaaatatccg
    47941 ctaccgagca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
    48001 ggttgtcgtt tggatagaga actttggtca gtttttctgc gttgatgccc tgctgttcgg
    48061 cacgcaggaa atcaccatcg ttaaatttag tcagatcaaa cggatgcgca tgcgtcgcct
    48121 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
    48181 cttgaccggt aatggtaaat tccggctccc agcgtccgtc tttcgtcact ttaccgccaa
    48241 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ccgcgatgcc
    48301 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
    48361 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgagaaagc
    48421 acgccgccag acgtcccaga ccaccgttcc ccagcgccgg gtcgatctct tcttccaaca
    48481 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
    48541 gattcaacag attgttgccc gtcaggcgac caatcaaaaa ctccattgag atgtagttaa
    48601 catgccgctg attcgccacc ggtttggcaa atggctgagc acgcagcatt tcggccagtg
    48661 cttcgctcac tgccagccac cactgacgag gggtcatttc agccgcagaa tttaagccat
    48721 aacgctgcca ctgacgtgaa agcgcttcct gaaattgctt atcgttaaaa ataggttgtg
    48781 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
    48841 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
    48901 aggaaatgtg atctcgacca cttaaagcta gtgcaatcca caggattagc agcaaatcaa
    48961 tgccataccg cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
    49021 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
    49081 tcacgtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
    49141 ttcatcagaa atgaagaaga aacccttgct taaatgaaac tgatgaacat aaggaaaata
    49201 ccgtacaacc cagtattcac gctggatcag cgtcgtttta ggtgagttgt taataaacat
    49261 ttggaattgt gacacagtgc aaattcagac acataaaaaa acgtcatcgc ttgcattaga
    49321 aaggtttctg gccgacctta taaccattaa ttacgaagcg caaaaaaaat aatatttcct
    49381 cattttccac agtgaagtga ttaactatgc tgattccgtc aaaattaagt cgcccggttc
    49441 gactcgacca taccgtggtt cgtgagcgcc tgctggctaa actttccggc gcgaacaact
    49501 tccggctggc gctgatcaca agtcctgcgg gctacggaaa gaccacgctc atttcccagt
    49561 gggcggcagg caaaaacgat atcggctggt actcgctgga tgaaggtgat aaccagcaag
    49621 agcgtttcgc cagctatctc attgccgccg tgcaacaggc aaccaacggt cactgcgcga
    49681 tatgtgagac gatggcgcaa aaacggcaat atgccagcct gacgtcactc ttcgcccagc
    49741 ttttcattga gctggcggaa tggcatagcc cactttatct ggtcatcgat gactatcatc
    49801 tgatcactaa tcctgtgatc cacgagtcaa tgcgcttctt tattcgccat caaccagaaa
    49861 atctcaccct tgtggtgttg tcacgcaacc ttccgcaact gggcattgcc aatctgcgtg
    49921 ttcgtgacca gctgctggaa attggcagtc agcaactggc atttacccat caggaagcga
    49981 aacagttttt tgattgccgt ctgtcatcgc cgattgaagc tgcagaaagc agtcggattt
    50041 gtgatgatgt ttccggttgg gcgacggcac tgcagctaat cgccctctcc gcccggcaga
    50101 atactcactc agcccataag tcggcacgcc gtctggcggg aatcaatgcc agccatcttt
    50161 cggattatct ggtcgatgag gttttagata acgtcgatct cgcaacacgc cattttctgt
    50221 tgaaaagcgc cattttgcgc tcaatgaacg atgcactcat cacccgtgtg accggcgaag
    50281 aaaacgggca aatgcgcctc gaagagattg agcgtcaggg gctgttttta cagcggatgg
    50341 atgataccgg cgagtggttt tgctatcacc cactgtttgg taacttcctg cgccagcgct
    50401 gccagtggga actggcggcg gagctgccgg aaatccaccg tgccgccgca gaaagctgga
    50461 tggcccaggg atttcccagc gaagcgattc atcatgcgct ggcggcaggc gatgcgctga
    50521 tgctgcgcga tattctgctt aatcacgcct ggagtttgtt caaccatagc gaactgtcgc
    50581 tgctggaaga gtcgcttaag gccctgccgt gggacagttt gctggaaaat ccgcagttgg
    50641 tgttattgca ggcgtggctg atgcaaagcc agcatcgcta cggcgaagtt aacaccctgc
    50701 tggcccgcgc tgaacatgaa atcaaggaca tcagagaagg caccatgcac gcagaattta
    50761 acgctctgcg cgcccaggtg gcgattaacg atggtaatcc ggatgaagcg gaacggctgg
    50821 caaaactggc actggaagag ctgccgccgg gctggttcta tagccgcatt gtggcaacct
    50881 cggtgctggg tgaagtgttg cactgcaaag gcgaattgac ccgctcactg gcgttaatgc
    50941 agcaaaccga acagatggca cgccagcacg atgtctggca ctacgctttg tggagtttaa
    51001 tccagcaaag tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg
    51061 aaaaagcgtt ccagctgatc aacgagcagc atctggaaca gctgccaatg catgagtttc
    51121 tggtgcgcat tcgtgcgcaa ctgttatggg cctgggcacg gctggatgaa gccgaagcgt
    51181 cggcgcgcag cgggattgaa gtcttgtcgt cttatcagcc acagcaacag cttcagtgcc
    51241 tggcaatgtt gattcaatgc tcgctggccc gtggtgattt agataacgcc cgtagccagc
    51301 tgaaccgtct ggaaaacctg ctggggaatg gcaaatatca cagcgactgg atctctaacg
    51361 ccaacaaagt ccgggtgatt tactggcaaa tgaccagcga taaagccgcc gctgccaact
    51421 ggttgcgtca tacggctaaa ccagagtttg cgaacaacca cttcctgcaa ggtcaatggc
    51481 gcaacattgc ccgtgcacaa atcttgctgg gcgagtttga atctgcggaa attgtcctcg
    51541 aagaactcaa tgaaaatgcc cgcagtctgc ggttgatgag cgatctcaac cgtaacctgt
    51601 tgctgcttaa tcaactgtac tggcaggccg gacgtaaaag tgacgctcag cgtgtgttgc
    51661 tggacgcatt aaaactggcg aatcgtaccg gatttatcag tcattttgtc atcgaaggcg
    51721 aagcgatggc gcaacaactg cgccagctta ttcaactcaa tacgctgccg gagctggaac
    51781 agcatcgcgc acagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat aaattcgccc
    51841 atttcgatga gaatttcgtt gaacgtctgc taaatcatcc ggaagtgcca gagctgatcc
    51901 gcaccagccc gttgacccaa cgtgaatggc aggtcctggg gctgatttac tccggttaca
    51961 gcaatgagca aattgcaggc gagctggaag tggcggcaac caccatcaaa acgcatatcc
    52021 gtaatctgta ccaaaaactc ggtgtcgccc accgtcaggc cgcggtacaa cacgcgcaga
    52081 aactgctgaa gatgatggga tatggggtgt gagtttagcc ggataatgcg tcggatccgg
    52141 cttacatctt tacatcattc aatgctaacc cgcgttacgc catctgtttc tatcaaacca
    52201 aaccgcaccg gcaagaaacg ctccaccacg gcgatattgg tcagcagatg acatgaggga
    52261 tgggcgaccg taaattctcc cgcgcccgcc agcgccatcg gtagcaccag ttggtcggcg
    52321 agatattccc ccaccgccgc cgggcttgcc aggtagcgtt tcacctcttt caccaactgt
    52381 gccgcgacca cctcggcact gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata
    52441 ttttcacttt cgacttcaag cgagacggta ttacccggcc cctggtcgcg cggcaggtta
    52501 tgaatattct gttcatgcaa ggaaaaacta cccgccagtg tagcgatttc acgctcagca
    52561 acatggcgcg gtaactttcg cgaactttct gccagcgtca cgcggatggc cacctttgcc
    52621 actagcggac gcatcacact ggatacagtg gaagatgaga tcaaccgtct gcgctataac
    52681 tggcaggaga gtcgcccctc cacgcttacg gcgttgctgg gcgcagaggc agaaaacatc
    52741 gatctcttcg accgtatgca actggaacac gttatcgcta tctgccgcca ggcaaagtcg
    52801 ctttctgctg ccggacgtga actgtttgac atttcgcgcc agggtaaagc cagcgtcaat
    52861 gacgcggatc ggctacgcaa atacctggcg cgttttggtc tgacatggga agcaatgcag
    52921 gatcagcaca gctccagttg aatatgatgg tccttcagca cctgcatcac gctcgcaggc
    52981 ggcatcgtgt cggtgtagac agcatcgacc atgctgatgc tacccatatt gaccattgcg
    53041 ttacggccaa attttgagtg gtcgacaacc agcataacgt ggcgcgagtt ctcaatgatg
    53101 gcgcgtttgg tgcgaacttc gtggtaatcg aactccagta gcgagccgtc gctatcgatg
    53161 ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg agataaaatc gagcgttgct
    53221 tcgccaatga tcccgccatc gcggctgcgt aattcgccac cggcaagaat gatacggaaa
    53281 tcctctttta ccatcagcgt gttagcgacg ttgagattgt tggtcacaat gcgtaaattg
    53341 ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg tgccgatatc gataaacagc
    53401 gtcgagccat tgggaatttg ctccgccact ttgcgggcga tgcgctcttt ttcttcggtc
    53461 tgggtggcct tgcgatcgtg ccacggcgtg ttaaccgaac tggaaggcag cgccgcaccg
    53521 ccatgatggc gcaggatcag gttttgctct gccagctcgt tgagatcgcg gcgaatagtc
    53581 tgcgggctaa cggagaaatg ctctaccagc tcttcggtgc tgacataacc ctgctgttta
    53641 accagttcga taataccgtt gtgacgttgt gtttgtttca ttgataaatc cctggaatta
    53701 ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc gctaaaccca cggctaaccc
    53761 ggcgatatgt gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc cggcgacaat
    53821 ccagatcaac gcaaagataa ttaacccacg ttgcaggtaa atgccacttt gcggatcgcg
    53881 ttcgccacgt agccagacgt agcccatcag cgcatacacc acgccagaaa gcccgccaaa
    53941 ccacggcccg ctgaattttt gctgcacata gccgcttaac agggcgctaa tgagagtaat
    54001 gacaattagc ttaccgctac cgaggcgttt ttccaccgca ccgccgagat accaccacca
    54061 gagcaggtta aagaggatat gcatcagcga gaagtgcatt aacgcgtggg tgaagtaacg
    54121 ccagaactca aatttcagcg tcggatcgaa tggccaggcc agccataaca tcacttcctg
    54181 atcgccgaga atttgcatgg cgataaacac caccacgcag gcgatcatca tcacccaggt
    54241 taccggacct gcgcgttcac gcaaggcggc aaagaaagga taacggcgat aatgcaggcc
    54301 actgtcggta tgaccggact gccaactggc ggcgagataa cgtggatctg ccgggttttc
    54361 gagaaaacga gccagctccg cccgcacgcg ttcggcctgg gactcatccg ccagccagac
    54421 atcgctttgg ttatgttgtt gaatcgtgag gataacaccc tgtgtcgcca tgtaatcaac
    54481 aaacgcctgc gccactcggg ggttagcaaa agaggtaatc atcaacatcg ttgctgtcgc
    54541 ttattccaca caaaagggga cagtataaag cgttacgcgc cgtacgccac ctctgcggga
    54601 aactggcgct gccaggcttc aaaaccgcca tcaatgctgt agaccacatc gtagccctgt
    54661 tgcagcagat actgcgccgc gcctttgctg ctattgccgt gataacacat caccatcacc
    54721 ggtgtgtcaa agtcgttatc acgcataaac gcgcccagtg tgtcgttagt taaatggaaa
    54781 gcctgggtcg catgccccat tgcgaaactc tgtggatcgc gaatatcgac cagcaccgcc
    54841 tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa ctgatccatg
    54901 cgtctctctt tctttacaaa caagtgggca aatttaccgc acagtttacg tcgaagcggc
    54961 agataaacgc cataatgtta tacatatcac tctaaaatgt tttttcaatg ttacccaaag
    55021 cgcgattctt tgctaatatg ttcgataacg aacatttatg aactttaacg aaagtgaatg
    55081 agggcagcat ggaaaccaaa gatctgattg tgataggtgg cggcatcaat ggtgctggta
    55141 tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat gctggaggcg caggatctcg
    55201 cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc taccttgagc
    55261 actatgaatt ccgactggtc agcgaggcgc tggctgaacg tgaagtgcta ctgaaaatgg
    55321 ccccgcatat cgccttcccg atgcgttttc gcctgccgca tcgtccgcat ctacgcccgg
    55381 cgtggatgat tcgcattggt ctgtttatgt acgatcatct gggcaagcgc accagcctgc
    55441 cgggatcaac cggtttgcgt tttggcgcaa actcagtgtt aaaaccggaa attaagcgcg
    55501 gattcgaata ttctgactgt tgggtagacg acgcccgtct ggtactcgcc aacgcccaga
    55561 tggtggtacg taaaggcggt gaagtgctta ctcgtactcg tgccacgtct gctcgccgcg
    55621 aaaacggcct gtggattgtg gaagcggaag atatcgatac cggcaaaaaa tatacatggc
    55681 aggcgcgcgg cctggttaat gctaccggcc cgtgggtgaa acaatttttc gacgacggga
    55741 tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg cagccatatt gtggtgccgc
    55801 gtgtgcatac ccagaagcaa gcctacattc tgcaaaacga agataaacgt attgtcttcg
    55861 tgatcccgtg gatggacgag ttttccatca tcggcaccac cgatgtggaa tacaaaggcg
    55921 atccaaaagc agtgaagatt gaagagagtg aaatcaatta cctgttgaaa gtgtataaca
    55981 cgcactttaa aaagcagtta agccgggacg atatcgtctg gacctactcc ggcgtgcgtc
    56041 cgctatgtga tgatgagtcc gactcgccgc aggccattac ccgtgattac acccttgata
    56101 tccatgatga aaacggcaaa gccccgctgc tgtcggtatt cggcggtaag ctgaccacct
    56161 accgcaaact ggcggagcac gcgctggaaa aactgacgcc atattatcag ggcattggtc
    56221 cggcgtggac gaaagagagc gtgctgccgg gtggtgccat tgaaggtgac cgtgacgatt
    56281 acgccgcccg cctgcgccgc cgttacccgt tcctgaccga atcgctggca cgtcactacg
    56341 cgcgcactta cggcagcaac agcgaactgt tgctcggcaa tgcaggggcg ataagcgacc
    56401 tcggtgaaga tttcggtcat gagttctacg aagcggagct gaaatatctg gtcgaccacg
    56461 aatgggtccg ccgcgccgac gatgccctgt ggcgtcgcac gaaacaaggc atgtggctaa
    56521 atgcggatca acaatctcgt gtgagtcagt ggctgatgga atatacgcag cagaggttgt
    56581 cgctggcgtc gtaaggatga tgcccggttg cgaagtgcaa ccgggctatt tgaattcctt
    56641 agactattca ttgtaaataa ttgaataacg gccccagagc taaaatcgcc aaacttaagc
    56701 acgttaccca atcgccccac ttcttcctat ggcttctatt tttgctacgc cactggcaat
    56761 aatatcccaa cgcaaaacca agctgggcac taatcgggcc gacaaaatca gtccacggct
    56821 gcggccaaaa aggggaaaaa ttcaggggaa gccagacaaa tgtcagaaag aaatttcgga
    56881 tattttccag caacgctgat ttctctggag aaatataaag aatgaaattc gagagaatta
    56941 accagaaata gaccggagaa aaaaccagcc agcaatgtag cctgtttttt aattcaggag
    57001 gcgttctgcg ggagaattgc caaccgatgc cacccattac actgaaaaaa agtgcatatt
    57061 caaccaataa ctcaagttgt aattcctgaa gagaaatacg cacaaccagg agcggaaagg
    57121 agaatattat ttcagccaga aaagttaacg tgaatataag tacggctaag gcaataaccg
    57181 tcggccatac caataatggt acgataacaa aaccgaaaat tacagggtaa atataaagtc
    57241 tgattttatt catctttaat gtcctgatga ttcggatgat gcgtcataca tggcacataa
    57301 acactcatca atcatccact cgtcttccca ggcctgatac tgtgtgccct gttcggactg
    57361 cttaagcgga ttctcaatcg cctgccgcgc gcgttctttc aggatggatt gattattact
    57421 agcccaagta ttagtttgag tggtaatggc tttatcgata tcgtcctgat tatctctgat
    57481 ataaccgtca cgatacgtat tcaataaaca ggcaagagac gctgccgacc aaaatccggg
    57541 aactgaaata atggcatcaa gcaatggcga aagagcgata tcggcacgga aaaacatttc
    57601 cagcttgtac tgtaattcga cagcctcatc cccggatacc caatcagtca tatactggca
    57661 agcaaattgc tgcaatattt cccgttcatc ctcacgccag ttttctttac tggcaaggtt
    57721 gatacgccgt agagaaaata cttcgtgaat attgtcaaat tgcccctgcg caacaaactc
    57781 cagtaaacaa ggcagaaaat aacgaacctc atcgctgttt tgcggatcag gtcctggact
    57841 actattccag gcattgatta aggaataagg aatcgctcgc aacgatgtat tccgtaatgc
    57901 tttttgttcc tgttgactca gacaatattc acaacaaacc acaaattgct gcggcgccgg
    57961 ataacgacga aaaatatgat atatctgctc aattaatgcc tgcatggcag gagtggggtg
    58021 cataattacc ccctcgcccg ctgatcgtgc ataaacggct gatagataaa aagggcattc
    58081 gccgcgttca ggaaaatatt aatgatatcc atattgtaat cctgcttaat gcaacgtttt
    58141 atccatcggc acatagccca catcttccac caaactctgc ccctgcggcg ttaaaaacca
    58201 ctccaccaat ttttgtgtct ctgacgtcgt attttcccgc gttaccatta aggcatcgac
    58261 gatataggga tatttgtcgt tgcgaatatt ttccgcagtc ggtgcaatac cgttaatcgc
    58321 tagcagtttt atatttttat cggcattcat ttgtgttgcg taatagcgga aggtgtagcc
    58381 tattgcgttt tttgtattac ggtattcggc aacaacgtta atcattccct ccatcacgct
    58441 tgccacttcc gtttcctgcg gcgagatcat gcggacattt ttcatgacct gcgatagcat
    58501 caccgtctgg ctgccagagt cttccgggcg ttgccaggtc tggatctcct gatcgtcacc
    58561 gccaaccgcg cgccagttgg taattgcgcc actgaaaatg tcacgcacct gttgttcagt
    58621 cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac gggcaaatgg
    58681 cgtgtacagc aaagtgacgc ccgatttctc agcgcgtttt ttttgcccat cggaaggttg
    58741 cgccacgaaa ataatatcgg catcaccgtt aacgattttg ttatatgctt cttgcgtacg
    58801 agagttatcc agataatccc aaacgtgaaa atcctctggt attacactta atgcataaaa
    58861 tgcagaggca taaatggggt acgccgccgt agcgccatcg agtcgcggcc agttttgcgt
    58921 aaattgaatt tgtggtttac cacgcagtgg cgtcagttga ttattaagtt tatcgggtcg
    58981 ccaggcccag gtatcaatat tttcacgcac accgacaccg ggattaagct gtttatataa
    59041 ataagcctga tacccagcga caaaaccaag taagagaagt agaaacagga gtaagttacg
    59101 gcaacgtagc ccggacgtgc cggttaaagg atgccttgtc ggccagccgt atcccagcga
    59161 aaaactgata tgcgcgccaa cgggtataat gactatccac cagtagtcgc cactgattaa
    59221 tgagagtaac aaaacaccct gccaggaaag cataaggttt gccaaccagc ccgatcccat
    59281 ctggcgaaat tgataatctg ccagatgtag cgcgattatc cagccaacca gggttaatag
    59341 cgcgggtaag agaaaaaagc attgtcgcca ggcggctatt tctctttttt ctggtgcgct
    59401 atcaccagcc cacacgcgac cagagaaaaa tatgattatc gcccccagcg tcagcataaa
    59461 gacgttgagt agaagataaa ttgttggcgt aaagagtaaa ttgcaaacct caagaatagc
    59521 ggcaagcccg gcccccagca tggcaacaac cgctgccaaa aattgcgcca gtatggggat
    59581 gccaaaaaaa gtcattacca gcgaggtcaa aatccatttt ccgctttgca ttattctttc
    59641 cattcttgct gaatggcgaa attatactcc ccgataaccc tcgccccctc tggacacttt
    59701 tcctaaataa aggcgaaaaa aacgggacct tttggccccg ttctgttttt tagcgaactt
    59761 ataatctcac cggatcaata tgccaaatat aatcggcata ctctttgatc gtacggtcag
    59821 aagagaagta acccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgac
    59881 gctcgtagag ttcgtcaact ttatcctgac aatcgacata gctgcgataa tccgccagta
    59941 cctggtagtg atcgccgaag ttgatcagcg aatcgaccag atcgcgatag cgacccggat
    60001 cttccggact gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt
    60061 tctcgtagta ttcacgcggt ttgtagccct gacggcgcaa ttcttccact tcttccgctg
    60121 tgttaccaaa gataaagata ttgtcagcac cgacatgatc cagcatctcg acattcgcac
    60181 cgtccagcgt accgatagtc agcgcgccgt taagcgcaaa cttcatgtta ctggtgccgg
    60241 aagcttccgt ccccgccagc gaaatctgtt cagacagatc ggccgccgga atgatcaact
    60301 gcgccaggct aacactgtag ttcgggatga acacgacttt cagcttgtcg ccaatctgcg
    60361 gatcgttgtt gatcactttc gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt
    60421 aataggccga agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg
    60481 ggtcggcctt gatgcggtta tagcgggtga tcacgtgcaa cacattcatc aattgacgtt
    60541 tgtattcgtg aatacgtttg atttgcacat cgaacagcgc tttcggattt accaccacat
    60601 tcagctgctg ggcgatatac tccgccagac gctttttgtt ctccagcttc gcctgatgca
    60661 ccgcgtgatt aaccattggg aaatcacagt gttgttgcag atcattaagc aggctaaggt
    60721 cggtgcgcca gttgcggccc aggtgttcgt ccaacacggc tgaaagcgat gggttcgcta
    60781 ccgccagcca gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga
    60841 ttttcgcaaa gtcggcaaat agcgattgca ccatcaggtt agagtgcagt tccgatacac
    60901 cgttaacttt gtggctcacc acaaccgcca gccaggccat acgcacacga cgaccattgg
    60961 attcatcaat gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca
    61021 aggttttcag gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt
    61081 tacccagcat atcgactggc caggtttcca gcgcctcgct cattagcgtg tggttggtgt
    61141 aggagaagac ctgacaacac acttcaaacg cgtcgtccca gctaaattgg tgctcatcga
    61201 tcagcagacg catcatctca ggaatcgaca gcaccggatg ggtatcgttg aggtgaatcg
    61261 caattttatc cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt
    61321 cctgaatggt cgaggaaacc aggaagtatt cctgacgcag tcgcagctca cgcccggagt
    61381 aggtggagtc gtccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca
    61441 ctgccgcgaa gtagtcgccc tggttgaatt taccgaggtt aatttcacta ctggcttggg
    61501 cactccacaa acgcaacgtg ttggtcgcgt cggtgtcgta acctgggatt atctgatcgt
    61561 aagctactcc cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa
    61621 tgcgaccacc aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac
    61681 cgtattccag ccagtagtca ggcgactctt tctggctacc gttaacgatg ttctgcttga
    61741 acataccgta gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag
    61801 aatcgaggaa gcaagccgcc agacggccca ggccaccgtt accgaggccc gggtcatttt
    61861 cttcatcaat cagctcttcg agatttaatc ccatcgcttc cagtgcgccc tgtacatctt
    61921 cgtaaattcc tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg
    61981 acaggtagta aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc
    62041 gctccacgag acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg
    62101 cgacgaccgg atcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag
    62161 cttctacgct aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg
    62221 aaaactattt caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa
    62281 aatccattgc catagcctga cgttgcacaa accgccacag tgaaggacgg gaccacagta
    62341 cgaaagcacg tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc
    62401 cactggcgac gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc
    62461 caccggtgcg ccgcactaac ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc
    62521 cgcacggttc aaaacggctg ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg
    62581 aaaatgcttc gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg
    62641 caaggaaacc ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct
    62701 gctccagaag accaggtaag gcttccagca ccaggtcgag acctttctgg ctggtcagac
    62761 ggctcaccac tgcaaaaagc ggcactttat cgtcaacctt aagccccatt gcgatttgta
    62821 actggcgctt attttccgct ttatcttcca acgtatcgcg ggtgtaacgc gaggccaaca
    62881 gtaagtccgt ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac
    62941 gcccttcacg gtgacgctgt tgcaacagac cttccatacc gtaggcaaac tgcggttcgg
    63001 tgatctcgcg agcgtaggtt gggctgactg ccgtaatgtg atcggcatag tacagaccgg
    63061 ccttcaggaa agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg
    63121 gcaattggat gtcattcatg tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca
    63181 cagtaaacac cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg
    63241 catgccagtc gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca
    63301 tttctgcccc aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata
    63361 agttggtatc gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt
    63421 atatgccaac cccgttgtaa tgaccgaaca acagcgtgat atgtccggcg aaggtatcac
    63481 gacgggatac tacctgcgca tcggtcacgc cacggcgaat atcgggaaat gcaggcaaca
    63541 gtacgcgagc gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag
    63601 ccagaccgcc ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta
    63661 tcgctcctgt ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc
    63721 ttctgaacga tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc
    63781 cggaataaca caagcacgat cgatgacgca gcgacgcaga cggcacgagc gacctaccca
    63841 tacttccggt aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga
    63901 gaacagaacg gactgcacca ccaccgaacc ggagatcaca caaccgccgg ataccagtga
    63961 gttaagggtc atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga
    64021 ttcattgtag gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga
    64081 ggccagatcg aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg
    64141 ctcggcatcc gggtcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc
    64201 ggcttcggtg atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcgcg
    64261 atcgtcttct tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact
    64321 cgccagagat ttgcctggat cgttcggcat tgacggcggg ttagctggtt tttcaacgaa
    64381 ttcgataatt ttatcgttct catcaaccgc cataacgcca aacgcggagg cttcttcaat
    64441 cggtactggc atacaagcaa cggtgcaacg tgcgcctttt tcgacgtggt cgataagcat
    64501 acgcgagtag tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt
    64561 ataacggcgg ataatgtcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt
    64621 ttcccctttc attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt
    64681 gaagaatgac cagccgcgct ggatgtgctg caccagagtg tgggactggt actgggtgat
    64741 cacgcccata cgacggatcc cggagttgat gcagttagac agcgcaaagt cgataatgcg
    64801 gaacttaccg ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg
    64861 ggtaccacgt cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgtgccaa
    64921 cattaagtga tcgttcttct ctaaactaac catgactaac tcctttttta tcatctctgg
    64981 aacacacaca atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct
    65041 ccagcgaatg ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca
    65101 agcgtggcgt taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc
    65161 ccgttttgcc actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg
    65221 ccgtcgcctt cttcccacca gcgattctcc atcaaagcgg gaatgcgctt gcgcagatgg
    65281 attaacgcgg cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt
    65341 aattggttat cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca
    65401 ccggccagta acatcggcgt accctgggag agcaacaacg ttgttaacag ggcgtgaatg
    65461 ctgtcgcgcc gccgttcaac cagatcaaga gtaccgccta acccttcttt accatgattg
    65521 ttactgtaat tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta
    65581 tggttgaagc aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc
    65641 gcggcactcg gcagacgatc attacgttta aaaacatcgc tggaggcagc aaaacgcccg
    65701 gcaaacgccc ccagaggcaa atcataatgc agccagaaac gacgggctgc atcgcggaaa
    65761 tgatcgttcc actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg
    65821 atatcccacg gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg
    65881 gtaaacaacg gcgcatcctg acgaaactct ggcgtacggc ccatgactgc cgccagatca
    65941 aagcggaaac catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa
    66001 tctaccaccg ccggatgact caaattgagc gtgttgccac aaccggtcca gttgtgataa
    66061 tcgccgtctt ctcttatcca ataatagcta cggttgtcga tcccacgcag cgagaataac
    66121 gggccgtcga ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata
    66181 cccgctttat gcagtgcttt gattgcatcg cgaaactcat ccagcgccgt ttctggcgag
    66241 caggcatacg ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttacta
    66301 agccccatac gttgcagacg tggttcactg gcaaattgtg ccaccggcag cagttccagc
    66361 gcggtaatgc ccaactgttt caaatagttg atcatcaccg gatgccccag ggctttataa
    66421 gtgccacgga tctcgaccgg gatctccggg tgcaggtacg ttaatccttt gacatgggct
    66481 tcataaatga tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagtcatag
    66541 tgatcaatca ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca
    66601 ttatgaccgg cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa
    66661 ggatcaatca acaacttcgc cgggttaaag cgatgcccct cggcaggttg ccaggggcca
    66721 tgaactcgat aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg
    66781 tcgccactgt gccctggcaa gtcatagcga tgttcctggc cattggcatc aaagacacac
    66841 agctctaccc gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa
    66901 tgcgcgccga ggggagtggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca
    66961 gccagatagt ggccagaggt ggtagcgtca ggctcagtga atgttgacga ccgtggctgg
    67021 caatctcatc gctgtgtact gcgccgccat tgcctgcatt actgccgtga tagtgcatgg
    67081 aatcggtatt gaggatttca cgccatttac ccggctggtt aataccgaag cgataatcat
    67141 gacgcggcac cggtgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac
    67201 gcacaaagat cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg
    67261 ggtcaaaatc cagttcatgc attgctttat ggtggcggta ggtgtggttc agatcgcgca
    67321 ccagacgctg gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc
    67381 tggcgtcatg gttccactcg cggccctggg caaattcgtt ccccatgaac agtagtttct
    67441 tgcccgggaa tgcccacatc cagccgtagt aggcgcgcag gttagcgaat ttctgccatg
    67501 cgtcgcccgg catccggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca
    67561 acggcaggac gaagttttca gtgtagttgt agagcatccc gaaggtcagt ttatcgtgat
    67621 gatactgacg ataaatcggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga
    67681 ggttccactt gtaccagaag cccagaccgc ccatatcctg tggacgagaa acgccaggga
    67741 aatcggtaga ctcctccgcc attgtcactg caccggaaac ctgctcacca agaatacggt
    67801 tggtattacg caagaattca atcgcttcaa gattctcgcg accgccaaat tcgttcggga
    67861 tccactcccc ctctttacgg ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc
    67921 gcagcgcatc aataccaaaa cgttcaatcc agtaaagcgc gttaccgaca aggaagttac
    67981 tgacttcacg gcgaccatag ttgtagatca gcgtgttcca gtcctgatga tagccttcgc
    68041 gcggatcgct gtgttcatac aagttcgtgc catcaaattc ggcaagcgca aagtcatcgg
    68101 tcgggaagtg gcctggcacc cagtcgagaa tcacgttcag accagctgcg tgtgcggcat
    68161 caatgaaata acggaagtcg tcgcgagtac caaaacggcg ggtcggtgca tacaggccgg
    68221 ttggctgata accccaactg ccatcgaagg gatgctcgtt aatgggcagt agctcgagat
    68281 gggtaaagcc catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca
    68341 accagaaatt gttgtcggtg tggcgacgcc aggagcccag gtgaacttca taaatagaga
    68401 ttggcgcatc aaactgattc gcttttttgc gctcttcagt ctgcacaacc ttttccggca
    68461 gcccgcaaat aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg
    68521 acttcagacg caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat
    68581 gcgccccagg gataaacagt tcccagattc cgctctcttt acgcaggcgc atcggatgac
    68641 ggcgaccgtc ccagtagttg aattgcccaa ccaccgagac ccgacgggcg tttggagccc
    68701 agacagaaaa acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt
    68761 catacgggcg taggtgagta ccttcagata atagccaggc atccatttcc tggattagcg
    68821 ggccaaaacg gtaaggatca tcaatcaggt tttgctgacc atgccagaca acagccaact
    68881 gatagcggaa aaaattctta cgtcgcggaa tgacaccgct aaagaatccc cgtgagtcga
    68941 gacactccag ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag
    69001 cgtcgggtaa aagggcacgg acttccagtc ccgcggtggt tttatgcatc cccagtacgg
    69061 aaaaaggatc cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat
    69121 cggacatgct tgtcttcctg ttttattgtg tcaccccatc caactgaatt tttgacttct
    69181 ggttgtgaca tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc
    69241 ctcacttcag gtaaggctgt gaatactcgt gtattccgcc acccttaaag aatagccaat
    69301 gctctattta actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta
    69361 gcaggggcgg catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat
    69421 ccgacgcagc ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag
    69481 gaactctggt cccatattca gcttacgcag acggcctacc ggcgtggtca gcgtgccggt
    69541 aacggcagct ggggttagct cacgcatagt gatttcccga tcgtttggaa caactttcgc
    69601 ccacggattg tgcgcagcca gcagttcttc cacggtcgga atagacacat cttttttcaa
    69661 tttaatagtg aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc
    69721 taccggaatt acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc
    69781 ttcgcggctc tgaccgttat caagctgttt gtcgatccac ggaatcaggc tacccgccag
    69841 cggcacgcca aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg
    69901 ttcgatatcg agaatagcag aggacgggtt cgcgagttca tcagccacat ggccatacag
    69961 atggcccatc tgggttaata actcacgcat atgtcgcgca ccaccgccgg aagcggcctg
    70021 gtaggttgca acggacaccc aatcaacaag atcattggcg aataaaccac ccagcgacat
    70081 caacatcagg cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc
    70141 gtcggtaatg acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg
    70201 cagagatgat gctgcgtcaa tccagtaacc ttgccatccg ctttcacgaa gctttggata
    70261 gatttcgttg gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc
    70321 ctccagatca aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg
    70381 accaagctga gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac
    70441 catgcgttgc atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt
    70501 cataagcgtt ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcctggca
    70561 catctttcac catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa
    70621 gccatcagta atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc
    70681 agggaatttg agttatgaat gaaatcattt ctgcaacagt tttattgatc ctgattatgg
    70741 atccgctcgg aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac
    70801 ggcgggcaat catggtgcga gagttgctta ttgctctcct tgtgatgctg gtgttcctgt
    70861 ttgcgggcga gaaaattctg gcatttctta gcctgcgggc agaaaccgtc tccatttctg
    70921 gcggcatcat tctgtttctg atcgccatta agatgatttt ccccagcgct tcaggaaata
    70981 gcagcgggct tccggcaggt gaagagccat ttatcgtgcc gttggcaatt ccgttagtcg
    71041 ccgggccgac tattctcgcc acgctgatgt tgttgtctca ccagtacccg aatcagatgg
    71101 gacatctggt gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt
    71161 cttcgctatt tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg
    71221 gattgattct ggtgatgatg gcaactcaga tgttcctcga cggcattcga atgtggatga
    71281 aggggtaagg ttcaaacagg tgttgcacac aaccagacgc ggcacctgtt ttattacctg
    71341 aactgataat gaccttcgcc caatcggata acccttccct cttcttctgc gcggttcagc
    71401 cacacactaa tttgttcgat ggttaaacca gtacaagacg ccagcctttc aatacttatc
    71461 gattcctttg caaggatcgc cagttccgcc acaaagagtt gataaaaatc gacaggggta
    71521 tgcgcgggca ttactgcgta atttgcgtca tcttcgtgca gcgaaaacag ttctggttga
    71581 gcgttacgga gtgctctgtc atacatcagc ccaggaacgt ctggcagtct gatcgtcaca
    71641 ttctcagcct gttcagcact ccatgacgcc ccattttcta ctaatcgtga attggctgat
    71701 tgcatatcct gatttggttt aacctgcatg ggcagccact gatgttttaa tgcctccatc
    71761 gccccggtta tcgttccacc ggtcattccc gcacgtacaa ccattgcgct ttcagcaagg
    71821 caataaatat atttattccg cgccatcgca ttgccgacgg tgaaacgtac ctctgggtaa
    71881 aacggcgaaa tcaacaccag gttgcctgct ataagccctt cacgccattt cactaacgta
    71941 ctcgttttta acaagctatc agctaatacg ccaacggcag ttcccccgac ctccagagcc
    72001 gaggccattg cacattcatc aatacctcgc gcaccaccag agataacgca aatcccctgt
    72061 tgggccagtt tcgcggccag ttgttgggta tagcgcaaat cgtcagtcgg agcatctcgc
    72121 gagccaacaa tcgccatacc ttccgcttgc agtaatgctt tattcccgca gccaaataaa
    72181 acgggaggtg catccgttcg caatcggttt ttcaagcgaa cgggataatc ggcatctccg
    72241 cgggttaaga tccataaacc ggcgcgttgc cacttatcta cagccaacgc cagactatga
    72301 ccacgcgcca gcaagccaag aattcgctcc tgagaaatac gcggatcgct ccagcctgta
    72361 agtttctctt gcggttgcgg caccagaagc tcggcgggac tgatacgttg gtgcttcagc
    72421 cataatgcaa acttccccca ttcactatta ctaagaggtt tatactcact cgccgccgcg
    72481 cgagaaaaat cgctggttag caggagagtt gcttgagcat tggctgaaag attcatcaat
    72541 tttttaccga ggtagaagca agggcaagag gataaaccgt cgggcaacct gcctggcgta
    72601 acagtgctgt cagaactgtc agcgtccatg cagaatccac gatatcatca accagcaacg
    72661 ccggacctgg catcaaagga ggggtaatca caaacgcccc gtcgagattt tgacactggt
    72721 gaaaacggtt ttgctgcatt ttctgcggtg gattgtccac gactttttca atggcatcaa
    72781 taaaaggtaa gccaagtttc gccgccagtc gccgggcaaa atcaggaacc aggtcgaggt
    72841 gccttaatga aggaacgcag catacccagg tgggttcagg atgcggattc cagcgttggc
    72901 gaaccatttc cgcacatgcc tccaccagct catcactgaa gcgccccgtt tttttctcct
    72961 gggctacctg ctttccccac ccggaatctc cccaacgaga aaggattcgc cccgtagatc
    73021 cttgtaaacc cgcaggcaag ttccctttaa atccgtattg cgtaaaagcc ccagatgcca
    73081 cctgtttatt gagatttaat ggcaggtcag cgtgtttaat aaataaattt gccgcatgga
    73141 gtaagtcgct atcaatgtcg ggcgacaaca gcggatgctg aagacaactg ctacatttac
    73201 cacaacgttc ggcacttggc tcatcgagtg cgcggcgcag aaattgcatt ttgcactcct
    73261 tggtcgtgat atagctttca acgtctgcca actcactttt tctctgattc atcaaatggt
    73321 taattcgttc atgatcagga gaaaaactga cgatagttct gcgccataat ttctcggtat
    73381 acaccacagg cgatggattt tcagctacca ataatttcag tgctttttct atttgcccat
    73441 aacgaagatt cgtccgttgt tcaatgcctc gtagggtaag accgtcattc tcgctaagta
    73501 cgttgagtat ttcatgaatt tgcgcctccg caggaaaggc gctttcacgg aagaattgat
    73561 gaatagcgcg gtcttcacca ccacaaagca atatgccaac tgcggaatct atagcacgcc
    73621 cggcacgccc cacctgttgg tagtaaccga caatagaccc cggcatctga taatgaatga
    73681 cgaaacctaa atcaggttta tcaaatccca tacctaatgc cgtcgtcgcg accagcactt
    73741 tgattttatt tgccagcagt gcctgctcca gatactccct ggcagtatta gaatcttccg
    73801 caccttcaca agtcacgccg ctgtaataag caaatgcact gataccattt ttccttaacc
    73861 actcggcgac aagttcagca tcgcgagtcg tcagggtata aacaattccc gatttggaaa
    73921 actgagggat aaccgttgct aaccatgcca gacgggatga ctgttctcct aagactaagg
    73981 catcaagcgc caaactttcg cgagccagcg ttccacgctg aatcacaatg tcacccaatt
    74041 gctgacggat atcctcaacg acacggttat tcgctgtcgc ggttgtaccc agaatagggg
    74101 tattcgcagg cagttggcgc aaaatatcta atatacgtcg gtaatccggg cggaaatcgt
    74161 ggccccaatc agagatgcag tgcgcctcat cgaccaccag taaaccgatg cggtcagcga
    74221 ctggatataa aaccgtttca agaaaatcct gattcgccaa ccgttcaggt gaaatcagca
    74281 gacaatctac gccaccttgc agcaacttat cgctaatgcg ctgccattct tccctgtttg
    74341 tagaattcaa cgtttcagcc gtaataccta accgttcagc ggcggcaacc tggttacgca
    74401 tcagggcaag caaaggagag atgataattg tcgggccagc gccgcgatcg cggaagattt
    74461 tactggcgat gaagtacacg gcacttttcc cccagccggt gcgctgtaca accagtagtt
    74521 ttcgccgctg attaaccacc gcatctatag cttcccattg cccttccctg aaagtagcgc
    74581 tggtgttact taacatccgc tgtaacagca attcagctcc gtgtttctcc atcatcttct
    74641 ccctcgtccg ttaacacagt gattagaacg gataatgttc cggtcttttt gcgagagaga
    74701 tcaaaaagat gaagacagga gaaaagagaa atgaattact gcgagcaagc tcgcagtaat
    74761 ttgtcgcagt aattaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt
    74821 gccgaggatg gtttccatca tggtccaggt tttaagcgtt tcggcttcgg tcgcgccggt
    74881 aaatttaccg aacaaccaga aacccgcgtc gttaacgtgg ctgacaacaa tcgaaccacc
    74941 ggcgatacaa atcgacagtg ccgccatttg cgcaccggag tagttcagtt gttcaatgac
    75001 cggcattacc aatcccaccg ccgttaaaca ggcaacggta gcagaaccct gaatgatgcg
    75061 cactgcagct gccagcacga agcaggtgat ggcaatcggc aggcccatgc cggttaacgc
    75121 ttcgcccagt gccggaccga caccagagtc aaccagcacc tgtttgaaca cgccgcccgc
    75181 accaatcacc agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac
    75241 tttgtctttt ggcataccct gacgcattgc cagaccgtaa atcgccacca ggcaagcaac
    75301 cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg tagaaccttc
    75361 tggcacaaaa cgcgcggcaa tggttttcag ccccaccagc accagtggca acaggatcag
    75421 cgacaggctg aatccgaagg acggcatttt gccttcgccg agatgcggtt cgctgatgtc
    75481 gtcaggaata tgcaactcca cgtagcggct gatgaaatta ccccacagcg gcccggcaat
    75541 aatcattccc ggaattgccg cgcacaggcc aatcaggatc atccagccaa agtcggcgtt
    75601 catctgcgat gccagcagca ttggcgctgg cccaggcacc aggaacgcag ccgctgccgc
    75661 cacgccggcg aataatggga ttaccagctt caccaggttc gtgccggtgt ggcgcgccat
    75721 tgagaaagca acgctaatca gcagaacaat cgccacttca aagaacagcg gtagcgcaca
    75781 aaccagcccc gcaaggccga tggcataatg cacgcggctg tgaccgaagg atttgagcat
    75841 tttgacggca atctgatcga ccgcgccggt ttcatgtaag atcttaccaa acatggctcc
    75901 cagggcgaca accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc
    75961 tgcgatttta tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccattaa
    76021 agccaggaaa gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc
    76081 tactgctgtt aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc
    76141 aatggtgctt gccacaacac cttccagcgg ttgatcaata tccaccacca gtacatcggt
    76201 ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa
    76261 gaaatggcct ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc ctttcaggta
    76321 gatgaaagag agattaggat taccttcacg cagcaagtcg cgatagtgtt ttttcagtgc
    76381 agaacagacg atcagcgata ctttattggt gcgctgcata gcaaacgcgg cgtcgttcag
    76441 cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt tcgccagacg ccattttttc
    76501 gatattgcag cgcggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc
    76561 cacttcactg gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg
    76621 gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta
    76681 cgggtaactg ttatcggtaa cattgtccag ccggacaata tcagaagcaa tatccatccg
    76741 tgccttaagt gtataagtgt gagctacttc aaatttgtag gcttaaatag atccgcccgg
    76801 tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc
    76861 cagcaggcgc tcagcgccaa tgctgcccat ccgctcacgc ggcgtcagca cactcgccag
    76921 ccgtggctcc atcacctgac caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc
    76981 aggaactttc aaccccagac gctgacattc aaacgccgcg ccgaccgcca ggtcatcatt
    77041 agtacagaac acgccatcca gctgcggata ttcccgccgc gcctggcgaa tcagttcaat
    77101 accggaagag taagaagaag attgctcaac catcacgcta tacggcacca ggcctgcatc
    77161 cagcatcgcc tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgtgcgcc
    77221 gagataggca atgtggcgat gcccgcgagc aataatggcg gtggtcatct ggcgtgccgc
    77281 ttcaaagtta tcaaaaccga cggcgatatc gagacacggc gacttactgt ccatcaactc
    77341 caccaccgga atccccgcca cttcaatcat ctttaaggtg cgtggcgtgt gggtacgttc
    77401 agtgaggatc aggccgtcga tattccatga gagcattgat tcgaggcgtt cttgctccat
    77461 ttccggttta taaccgtagt gcgccagcat ggtttgataa ccgtgcgcgt cggtgacact
    77521 ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc
    77581 aatcgcccgg ctggtggcgt tagagagtat atcgggcgcg cgattgggaa tataacccag
    77641 ttcatcaaga gcggcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg
    77701 taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac
    77761 gggtcttttc tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca
    77821 cggacgcatc acaaccttcc tgattcctca ggaaggtttg tgagataaat ttagactggc
    77881 ggtaaatcga acagcaggat ttcactgtcg ctatcagcat ggatggaaat cgcctgctca
    77941 tcccagattg ccagaccatc gctggtcgaa gcttttacgc cgttaatggt gacattgcct
    78001 ttcaccacct ggatccagat gcggcgttca gcggcaatct gatgcaccga ctgctcatct
    78061 ttcaccagcg cccaacgata cagttccata tcctgatgca ctttcaatga accatctcgc
    78121 gcatccggcg aaagcaccag ttgtttgccc tgcacggcat cgaagcgacg ctgttcataa
    78181 cgcggcgtaa taccgttttc ttcaggcatg atccagatct gatacagatg cagacgctcg
    78241 gtgctgcttg ggttgtactc tgagtgacga atacccgtac ccgcactcat aatctggaac
    78301 tcgcccgccg gaacctgctc tttattgccc atgctgtcct ggtgctcaac agtaccttcc
    78361 agcacgtagg tcaaaatttc catatcttta tgcgggtgag tgccgaagcc ctgccctgct
    78421 tcaataacgt cgtcgttaat cacgcgcagc gcagagaagc ccataaagtt cggatcgtaa
    78481 tagttggcaa aagagaaagt atgccaggag tccagccagc catgatttgc atgaccgcgt
    78541 tcatttgctt tgcgtaagta gatcatttgt attcgccccc tgaatgattt cgatgggctt
    78601 agtgtggacc cgatccgcct gggatgatag agggtgaaaa ttgacccctc tgttcaaaaa
    78661 aattgaacta ttcgaggggc catcaaactt acctcgcgag actcactgcg gagggagaag
    78721 cttgctcaaa accgcgttca aggatttcca gattcgtaag aacttcagat tccttgacgt
    78781 aatttggcgc accgttggta atggtttgat acaacgcatc ataaacgcgc ccataatcgc
    78841 ccatctctgg cttcatctct tctcttaccg tcacgccctc gtcattgaca tactccagca
    78901 caccgaccga atcatccgct gcgaatcctg gttcgcccgg cataatatta gccttcaggc
    78961 tggtttcctg ctggtcgata ccgtatttaa taaacgaacc tttcttaccg tgaacgataa
    79021 atttcggata atcgattttc accagatggc tggttttgac gatggctttt agatcgccat
    79081 aaaacagctg cgcttcaaag gtgtcgtccg gattggcttt attacgcagg ctgcggatgt
    79141 cataagcgac gtgatccggg cgaccgaaca gagaaataat ctggtccatc gtatgcacac
    79201 caaggccata gaacgcgcca tcctgcggca gccccggttt ggtttctgcc accgggcggt
    79261 aatagtcaaa atggctttcc acttcgacaa tctctcccag cttgccgctt tcaatcgctt
    79321 ttttcgctgt caggaagcag gaatcaaagc gacgattctg atacggcgta acgatcagcc
    79381 ctttgctttt cgccagtgca aacagctctt tcgcctgcgc aattgtcgga gtgaacggtt
    79441 tttcgaccag cacatttttc ccggcttcca gcgcgcgctt cgcgtactcg aagtggctgt
    79501 ccgcgtgggt gcagacgaca accagcttaa catcgggatc gtttagcact tcgtcgagat
    79561 cgctggtgaa atggatatgg gaataaatgg gggcctgttc ttccggcttc gcatggcggc
    79621 gaaaaatatg cgcgacatgc cagctatcct tgcggttaag tacatacggc agatggtaac
    79681 gggtggtgct tttgccgaag ccaataaagg cgcagttaat gaccatgatt tcgtcctttt
    79741 taaggtggtt atttacacct tagcgcaaag cggacgtggt tcctactgga gtgcgcataa
    79801 cgaacacaag cacttcccga ggataaattg agaacgaaag atcaaaaaaa aagccagcac
    79861 ccggctggct aagtaatact ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc
    79921 gagggtcttc ctgatcgcga gacaataata atcattctca ttcgcacttg tccaacactt
    79981 tttgcaaaaa aatgcatttg actcgcattt gaaagtcaat gatgttgaaa gggacattta
    80041 ccccaaagag gacaaaggaa tgagtgagat agtaatacgc cacgcagaaa cacgcgatta
    80101 cgaggccatc aggcagattc acgcccagcc ggaggtgtat tacaacacgc tacaggtgcc
    80161 tcatccttca gaacaaatgt ggctggaacg actcaccgct caccctggca tcaagcagtt
    80221 agtcgcctgt attgatggaa tcgtcgtagg ccatctcacc attgacgtgc aacaacgccc
    80281 acgccgcagt catgttgccg attttggtat ctgtgtcgac tcccgttgga agaaccgcgg
    80341 cgtcgccagc gccctgatgc gagagatgat tgaaatgtgc gacaactggt tgcgggtaga
    80401 tcgtatcgag ctaacggtgt tcgtcgataa cgcgccagca attaaggtct ataaaaaatt
    80461 tggctttgag attgaaggga ctggcaagaa gtacgcatta cgaaacggtg aatatgtcga
    80521 tgcgtattat atggcgcgtg tgaaatagag aatttgcaat ctacgtttgt catttaagcc
    80581 ctcacctacc gggagagggc ttaagatatt aataccccgc cgttaaatca tccaccgagc
    80641 gcgggtcgga tgcaccgtac agctcaccgt ccggcccaac cataatgctt tgtgtactgc
    80701 ccatcgcctc tttcagcgcc actttctgac cttttgcttc cagcagcttg atcgtatccg
    80761 ggctaaaccc tttttcgaca cgcagctcgt ccggcaacca ctgatggtgg aaacgcggcg
    80821 cattggtcgc ttcggcgacg ttcattccat aatcgatgct attcaccacc atttgcagca
    80881 cagtggtgat aatccggcta ccgcctgggc ttccggtgac cagccaggtt ttaccatcct
    80941 tgaccacaat ggttggtgac atcgacgaca gcgggcgttt attcggcccg acggcgttgg
    81001 catcgccgcc caccagcccg taaacgttcg gtacgcttgg tttggcggag aaatcatcca
    81061 tctggttatt gagcaaaata ccgctctcgc ccgcgacaat gcccgtaccg aaggtggtgt
    81121 tcagtgtata ggtcaccgcc acggcgttac cgtctttatc cactactgag taatgggtag
    81181 tttgattact ctcataaggc gcaagcttgc caggacgaat ttcgctggat ggcttcgctt
    81241 tattgatatc gatttgatcg gcaatagatt tggcataggc tttattggtc agcgcctgcc
    81301 acggtacttt gacaaaatcc gggtcgccaa gatattccga gcggtcggcg taggcgtatt
    81361 tctcagcttc tgccatgatt tgcatcgcgt cggcgctgcc aaagccgtac ttctgcatat
    81421 cgaagttttc cagaatattg aggatttgta cgatatgaat accgccggaa gatggcggtg
    81481 gcatggagta aacctgatac ccgcgataat cgccgcttat cggtgtgcgt tcgaccgctt
    81541 tatatgccgc taaatcttct ttgctgatca agccaccgtt tttctgcatc tcctgggcaa
    81601 tctgttccgc tatcgtgcct ttgtagaatt catccgggcc gttttcggca atcatctcca
    81661 ggctctttgc caggttcgcc tgcaccagct tgtcgccctt tttcagcggt tcgccctctt
    81721 tccagaagat agctttgctg ttttcgtggt tcggcagcac ttcactgccg taggttttga
    81781 gatcgtcagc cagcgcgtcg ttaacgataa aaccatcgcg tgccagttta aacgccggtt
    81841 gcaccacttt attcaacggc atggtgccgt atttatccag cgccaacgag aaacccgcta
    81901 ccgtaccggg tgtgccggaa gccagatgcg aagtgagtga ttttttgctg tccgtgttgc
    81961 cctgatcatc gaggaacata tcgcgggtcg ctttggcggg tgccatttcg cggaaatcga
    82021 tagccgtggt attgccattt ttcgagcgga tcagcataaa accaccgccg cccagattcc
    82081 ctgcctgagg atgcgttacc gccagcgcgt agcccaccgc cacggcagca tcaacggcat
    82141 tcccgccctc cttgaggata tccactccca cctgagtggc agtaacatct acagacgcca
    82201 ccatcccttg tttcgcgcgt accggatgga agacatcttc ctccacacca taagaaacag
    82261 gaggcgcggc taccgtacta aaacaacttc ctgtgagcag agcagcaatg gccacccggc
    82321 gtaaaaacgt cggttttatc atcgttattc tccagagatt aaggggcaac cccagctaag
    82381 cctggtatat aactctgaat taatcatcgt tttgccggga agcgagtaaa cttaaagaat
    82441 atcttcagag gaggatacgc gatgaaacga cttctgcttt tgacggcact cctgccgttt
    82501 gtcggctttg cacagcccat taatactctg aacaacccta accagccggg atatcagatc
    82561 cccagccagc agcggatgca aactcaaatg cagacgcagc aaattcagca aaaagggatg
    82621 ctgaaccagc aactgaaaac gcaaacgcag ctgcaacaac agcatttaga aaaccagata
    82681 aacaataatt ctcagcgggt gttgcagtca caaccggggg agcgaaatcc cgcccggcag
    82741 caaatgctgc ccaacaccaa cggcgggatg ttaaacagca accgtaatcc ggatagttcg
    82801 ttgaatcagc agcatatgct gccggagagg agaaacggcg acatgctgaa tcagcccagc
    82861 acgccgcagc ctgacattcc gttgaaaact attgggccgt aaagttcgga ccaatcacgt
    82921 caatcgcatc ggtacagatg caatccacgc cccagcgcag caactctgcc gcgcgctggg
    82981 gtttattgac ggtataaacc agaattcgca gtcctgcatc tttcaactgc atcactcgcg
    83041 ctttatcgag caacttataa ttgagatgaa tagagacgca gcccagccgc gcggtcaatt
    83101 ctcgccagtc gtcgcgccat tcatcaagca ataaaccacg cggcagttcc ggtgctgcct
    83161 gttgagcggc ttctaaagca tcaatctcaa acgatgacag cagcggcggc gtcataccgg
    83221 tccacagttc gcgtgccgcc aacgccacca ttttgcccgt taatggtcca gtgtcagtgg
    83281 tgggtttgat ttcgatattc gccatcatcc cgtgtttgcg acagcgttcc gccacctgcg
    83341 aaagcaacgg cagtggctca cctttaaacg ctttgctgta ccaactgcct gcatccacgc
    83401 gcagtaaatc ctgccagttc agttcacccg cgacgcccca gccgttgctg gtgcgttcaa
    83461 gattgtcgtc atggagcagg aagatctcgc catctttcga taacttcgcg tcaaattcga
    83521 tcatcttatg accgtatttt gccccgacgt cgattgctgc cagggtgttc tccggggcca
    83581 gtttaccgcc gccacgatgg gcgacgatgc ggggataagg ccagttactc atactcgttg
    83641 tcctgtttca ccatcaaaaa gatgtagctg attttccggc agatgcagcc acagcgtgct
    83701 gcctgccgtc gggcgctcct gatgtgccag tcgcaccacc agcttctgtt cgccccaatg
    83761 tccgtgcgcc aggttatctg cgccgaggat ctccagcgtg tccatcacca gcggcacacc
    83821 accttctgcc tgcgagctta gcgctatatg ttccgggcga atgccgaggg tcattttacg
    83881 cccgacatac tgacggtagc caccgtttag cggcagcgca ataccgccat ccagttcgaa
    83941 atgcgtacct tcgttattca cacggccagc cagtaggttc atcgccgggc tgccgataaa
    84001 actcgccaca aacaggctgg cgggcttttc gtacacttca actggcgtgc caatctgttc
    84061 ggcaacgccg ccgttcatca ccattactcg ctgggcgagc gtcatcgcct caacctgatc
    84121 gtgggtaacg tagagtgaag tcgttttcag gcgacggtgc agctgttgca gttcaagacg
    84181 catctgcaca cgcaacttgg catcgaggtt agagagcggc tcatcaaata ggaacactgc
    84241 cggctcacgc acaatcgcac gccccatcgc cacacgctga cgctgaccgc cagatagctc
    84301 acgcgggcgg cgcttaagca ggccgtccag ctccagaatg cgcgccgctt ctttaacacg
    84361 ttcggcaatt tgttgcttgc ccatgccgcg aattttcagc ccccacgcca tgttctcctc
    84421 tacgctcata tgcggataga gcgcgtagtt ctggaacacc atcgcaatcc cgcgatcttt
    84481 cggctccatt tcggtgacac gcttgcggtc gatccagata tcgcccgtcg ttacccgctc
    84541 cagtccggcc accattcgca ataacgtcga tttcccgcag ccagacggcc cgaccatcac
    84601 gataaattcg ccatccgcca catcaagggt cagcggttta atgacctggg ttttgccatc
    84661 ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca ctatcgacca
    84721 ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac tactggtggg atcagggtta
    84781 acaacatcgc cgccatcacc gagttccatt ccgttgtgcc ttcgcctgtg gcgatcatcc
    84841 ctttaatccc tgccacggtg gtgccgagat caacgtcggt aataatcaac aacggccaca
    84901 aatactggtt ccagccgtaa ataaaggtga tcacaaacag cgccgccaga ttggttttgg
    84961 acagcggaaa aacgatgtcg caaaagaaac gcattggcga cgcgccgtcg atccgcgcgg
    85021 cttccaccag ttcatccggt agggtcataa agaactggcg aaacagaaac gtggcggtcg
    85081 ccgaggccat cagcggcagc gttaaacctg cgtagctgtc gagcattttc aggttggcga
    85141 tgacttccac cgtcgggaag atacgcactt caaccggcag catcagggtg ataaaaatca
    85201 tccagaagaa gaggttacgt agcggaaaac gaaaccagac aatggcaaat gccgagagca
    85261 tcgagacggt aattttgccg agcgtaatgc tgaacgccat cacaaagctg ttaagcaaca
    85321 tccgccagaa cggcgcgcta ttcgtgccta ccccgtttac ccagatgttg tggatgtttt
    85381 ccagcagatg tgtgccggga ataagcgtca tcggcgcggc gtagacttct tgtttatcca
    85441 gcgtcgccgc gacaaacgcc acgtacagcg ggaagaggat caccgcgatc ccgaggatca
    85501 gcatggtatg gctgaatatt gtcagccacg gacggttctc aatcattggt aacgtacctt
    85561 gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca tcaacaccac
    85621 cgactgcgcg gcagacgaag ccaaatccag tccggtaaag ccttcacggt agatcttata
    85681 aatcagcgtc gtggtggcct gtactggccc gccggacgtg gcggcgtcga tcaccgggaa
    85741 ggtgtcgaag aaggcataca ccaggttcac caccagcagg aagaaactca ccggagcgat
    85801 aagcggcagc gcaatcttaa agaagcggcg aatcgggcca gccccatcaa tggctgcggc
    85861 ttcgatcaac gaacggggga tggattgcag cgcggcatag aagaacagaa agttgtagct
    85921 gatttgcttc cacactgagg caaacaccac caggaacatc gcctgaccgc tgttttgcgc
    85981 gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc gaccggggtt
    86041 aaacagaaag atccacaata cggcggcaac ggcgggagcc acggcatacg gtagcaacat
    86101 caaggtttga tagaaacgac tgccgcgcac gatgtactcc accagcgctg cgaagaacag
    86161 cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg tccagaaggc
    86221 gtcgagatag tagctgtcgt gaaacagcgt gacgaaatta tccaggccga caaactggct
    86281 ggagaaacca aacggatcga cgctttgtag cgagtaccac aacgcttcgc ccgcaggcca
    86341 gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcaaataag gtagccagcg
    86401 cgagcggaac accggacggg atgatggcat tactgttaat tcctgaactg tgccggatgc
    86461 gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc atccgacatt
    86521 acactgatta agacttcgtc gatttctcaa agcggcgcag taactggttc ccacgctcaa
    86581 cggcagtatc cagcgcctgc tgtggtgtct tcttaccggt ccacacgctc tccagctctt
    86641 catccacaat cacgcggatc tgcggcatgt tgcccagacg caaacctttg gtgaacggca
    86701 acggtggctt gttcagcatc tgacgcgtgg caatatccgc tcctgggttt ttctcgtaaa
    86761 agccctgctc acgggtcagg tcatacgccg ctttagtgat tggtagataa ccggttttct
    86821 gatgccactc ggcagcgttt tctggttttg cgaggaagtc gaggaacttc gccacgccgg
    86881 tgtaagtttc tttatcttta ccctgcatca cccacaggct ggccccgccg ataatggcgt
    86941 tttgcggcgc gtctttcgca tcggcgtcgt aaggcatcat gcctacgcca tagttaaatt
    87001 tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtcgtcatc gcgcaatcac
    87061 cgttatagaa cttctcggtg gattcatcct tacgcccaac gtagctgaaa tcgcccttct
    87121 tgttcatctc ttcgagcatg gcgatgtgtt tcacctgctc cggcttgttg aactcaagca
    87181 ccgcgtctgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg ttccaggcgc
    87241 taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac ttcatgccgg
    87301 aggcttttag ttttgcggca tagtccgcca gatcctgcca ggtttttggc ggctgttccg
    87361 ggtctaatcc tgctttcttg aaggcgtctt tgttgtaata gagaacgggg gtcgaactgt
    87421 tgaacggctg ggaaagtaag tgcccagttt tgctgtcgga gtagtaaccg gaaaccgtcg
    87481 gcacaaactg cgactcatcg aattgaatcc ttgcctcttt aaatacgtca tacaccggtt
    87541 taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc aaaatagccg
    87601 gagcgttgcc ggtacgaaat gcggcaatcc ccgcgcttaa attctgttcg taattgcctt
    87661 tataggtcgg tacaattttg taatccgggt tttcggcgtt aaaacgttgg gccagagaat
    87721 ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc gtcactgcct
    87781 gcgcatttgc cattaaagcc agtccgagcg ccagtgctga agctgtataa cgtaacggtt
    87841 tcattgatta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta tgctcgcgga
    87901 gtaacatgac atgctcgaat tacagaaaaa taactatttt gttacatttt taagatagta
    87961 aggtgtcaga aagatgacaa ggcggtgacg gcgcgggcga gggaaaatgg gagatggggc
    88021 acggataagc ggaaaaatag aaggtctgaa tcaaactcta cagattactc atcgtttcat
    88081 gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc aatatattgc
    88141 aactcaccca gtaggcctga taagcgcagc gcatcaggca attttacatt tgtcacctgt
    88201 ctcaaaggag tcttttgact ccttatcaat caacgtgtta ttacccgcct aaatatgcac
    88261 ttctcactgc ttcattcgcc agcagcgcgt cgccggtatc ggagagcact acatgaccgt
    88321 tttccagcac gtagccgcga tcagccagct ttagcgcctg gttggcgttc tgctcgacga
    88381 ggaagatggt catcccctgc tcgcgcagtt gttcgatggt gtcgaaaatt tgctggatga
    88441 taatcggtgc cagcccgagc gatggctcat caagcagtag caaacgcggg ttactcatca
    88501 aagcgcgacc aatcgccagc atctgctgtt caccgccaga catagtgccc gctcgctgaa
    88561 cacggcgctc atgcagacgc ggaaacagtt catagaccca ctttatgcgc tcctggaact
    88621 ggtcgcgttc ggcaaaaaag ccgcccatcg ccaggttctc ttccaccgtc atccgcgaga
    88681 agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc gccgtctgcc
    88741 agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc ggatcgccgc
    88801 ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgagg gtgacaatct
    88861 cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg tagtgggcgc
    88921 tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa ataggcacgg
    88981 atcacatctg ggttattacg gatctgctcc ggtgtaccgt ttgccagcgg catcccctga
    89041 ttgaccacgt aaattctgtc tgaaatcccc atcaccagct tcatatcgtg ttcaatcaac
    89101 aagatagtgg tgttgtgatg gttacgcagt tcggcaatca gctcatccag ctctttcgtc
    89161 tctttcgggt taagacctgc tgccggttcg tcgagcatta aaatctccgg ctgcgtcacc
    89221 atacagcggg caatctccag acggcgctgg tcaccatagg ccaggttact cgcctgacgg
    89281 ttggcatgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc gagcgcttcg
    89341 ctctgggcgc gacggaaaga gggggttttc aacaggccgg agaacagccc agttttcagt
    89401 tgctgatgct gtgccaccag caggttttca atcaccgtca tttcacggaa cagacgtaca
    89461 tgctggaagg tgcgcaccac gcccatgcgg gcaatttgct gccccggtaa accttccagg
    89521 tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttat agaatccggt caggcagttg
    89581 aagaccgtgg tttttccggc accgttaggg ccgattaacg agacgatctc ctgcgggtac
    89641 agttcaagat tgacgttgtt caccgccagc aggccgccga agcgcatcat caggccgtta
    89701 acagataata atggctgact catgcctgct ctccttttgc tgcgccgttt ttcagcttga
    89761 gttgcgggcg cgtcatgggc agcaagccct gcggacgcca gatcatcatc agcaccatca
    89821 aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaactcg cgcgatacca
    89881 ccagcaaaac agccgcaaga atcactgcaa attgcgagcc cataccgccg agcaccacta
    89941 tcgccagcac aaacgccgat tcggcaaaag tgaaggattc agggctgaca aagccctggc
    90001 gcgcagcaaa caacgttccg gcaaaaccgg caaacgcagc gctgatggta aaggcagtca
    90061 gcttgatacg acgcgggctt aagcccagcg aacgacaagc gatttcatct tcacgcaacg
    90121 cttcccacgc acgccccagc ggcatccgca gcaggcggtt aatgacaaac aggcttaaca
    90181 ccactagcag caacgccacc aggtagagga agatgacgcg atcggaggga tcgtatttca
    90241 ggccaaagaa attgctgaac gtgtcccagc cgccttcgcg ggcggtacgg ctgaactcga
    90301 gtccgaagag cgtcggtttc ggaatctgac tgataccgtt cggtccgccg gtaatttcgg
    90361 tgttattgag cagcaatatg cgcacaattt cgccgaaccc gagggtaacg atcgccagat
    90421 agtcaccgcg cagacgcagc accggaaaac cgagcaggaa gcccgccgcc gccgccatta
    90481 acccggcaat cggcagacag gtccagaagc ccaggccgta atagtgattg agcagcgcaa
    90541 aagtgtaagc gccgatggcg taaaagccgc cgtatcccag caccagcaga ccagaaagac
    90601 caacaaccac gttcagcccg agaccgagaa taatgtagat catggtcaga gtggcgatat
    90661 ccaccgtccc acgcgacacc ataaacggcc acgccactgc aagcaccagc agcgccacga
    90721 ggaacagttt ctgcttcacc gtggagccat caatcgcggg cagaataaac ttcggcccgg
    90781 aaacgctttt tagccctttc tggaaagcag gccgcacaag ctggaagaaa aagaccaccg
    90841 ccgtgccgat aaacacccac tgccaacgga tctctgaagc cgtgtcgacc accagtttgg
    90901 tgccatccag ctccagttgc acgcccataa agacgcccgc cagcacaaag aacatagcgg
    90961 cagagagcag cgccattgca atatgcatcg gtttcatact ttctctacct ccgggcgacc
    91021 cagaataccg gtcggcatca ccagcagcac caggatcagc agggcgaatg agaccacatc
    91081 tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca ggatcagccc
    91141 gccaatcatc gcccccggaa tgctgccaat cccaccgagc accgcagcgg taaaggcttt
    91201 catcccggcc ataaagccga tgtaggggtt aattacgccg tagaactgac cgagcagcac
    91261 gcccgccact gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca cccggtcggt
    91321 gttgatgcca agcaggctcg ccattttcag atcttccgcg caggcacggc aggcgcgccc
    91381 catacgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaaag taacaatcca
    91441 tatcaccgcc tgcatggtgg taatagacgc agagaagttt tcgctatggc ccaccaccca
    91501 ctgaccgtta aacaggctgg gcagcgccac atcgcgcgaa ccttcggtca ggctgacgta
    91561 gttttgcagg aagatagaca tgccgattgc cgagatgagt gcaatcaggc gcttagagtt
    91621 gcgcaccggg cgataggcta cccgttcgat actccagccg taggcgctgg caatgacgat
    91681 tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca gcatcatcag
    91741 tgcggcgatg atcataaagg agacgtagct gccaatcatg taaacctcgc cgtgggcgaa
    91801 gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca gcgcgtaggt
    91861 actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact gctcagacat
    91921 aaggtaacct ttctaaaccc gcccgttttt cacggacggt gggatgatca cttggctgcc
    91981 gtggatgaac cgtcggcgtg ccactggaag acaccaaaat cgaatccctt aagatcgcct
    92041 ttttcatccc agttcagcgg cccaattacg gtgtttgcac cgttagcttt taaatctttc
    92101 accagcgcca gcggctcatc gctgccggta cgttcaaggg cagttgccag agattgcacc
    92161 gccgcgtagg tgatccagac atagggcccg gacggatctt tcttgtcggc tttcagcgca
    92221 tcaacgatgc cctggtttgt cggatcctgg tcatagcgtt ttggcatggt gaccaacatg
    92281 ccttcagcgg catcaccggc aatgttcgac agtgacgcat taccgacacc ttccggcccc
    92341 ataaactggg ttttcaggcc aacggaacgg gcctggcgca gcatctgccc catttccggg
    92401 tagtaaccgc cgtagtaaac gaagtcgatg ttttcttttt tcaggcgggc gatcagcgcg
    92461 gagaaatctt tctctccagc ggtaatgccg tcgaagaaga cgacgttggc gttagccgct
    92521 ttcagcccgt cctgcaccga acgtgccagc ccttcgccat actgttgttt gtcgtgaatg
    92581 atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt tggcccctgg
    92641 gaagagtcca gcccggcagt acgcatgatg tattgataac cgcgttgggt cagctccgga
    92701 ttggtcgctc ccggcgagat catcagaata ccttcgtctt catagatatc tgatgcaggt
    92761 tgggtagagg aggaacacaa gtgcccaata acgtatttaa tgccgtcgtt aacgattttg
    92821 ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac gccaaccagt
    92881 ttatcgccct taattccccc tttggcatta atgtctttaa ttgcctgacg cgcgccgtta
    92941 aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac ggcgacttta
    93001 atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc gatgatagtt
    93061 ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc tttgttctta
    93121 tgtgttaaca aatcagactg ttctttttta tactgcactg tttttacctg tctgattaag
    93181 ggtttagcgc agtattttgt gatgatagcg attaaaatcc ctatttttca gtcgattaag
    93241 aacagataat attctgaatt tattgataga taaacagaaa aaagcgccgt tgtcagcata
    93301 aaataacggt acaaagggca gaataattca ctaccattta ggggattatg ctggatattt
    93361 ttcattctct aatgttttaa ctttgtaatt attgctgaaa aaaaattaat cacctgccaa
    93421 aagaaataaa aaagagaaag cctccgatta aattatttcg ctacactggt tccacttttg
    93481 tgatttacac gggttcccca tgaagctgac catcattcga ttagaaaact ttagcgacca
    93541 ggaccggatt gacctgcaaa agatctggcc ggagtattcc ccttcctctt tacaggttga
    93601 cgataaccac cgcatctacg ccgcgcgttt taacgagcgc ctgctcgctg ccgtgcgggt
    93661 aaccttaagc ggcaccgagg gggcactgga ttctctacgc gtgcgggaag taacccgacg
    93721 tcgcggtgtg gggcaatatc tgctggaaga ggttttgcgt aacaatcctg gcgtttcatg
    93781 ttggtggatg gcggatgctg gcgtggaaga tcgcggcgtg atgacggcgt ttatgcgagc
    93841 actgggattt acggcacaac agggtggctg ggagaagcgt taatcgtctg gtgtaaaaag
    93901 tagctctata gatttggtcg ggaaaaataa cgcattttcg ttttctccga ccaatgggat
    93961 aaagcccagc gactgataaa acgtatgggc tttttcattc agcgcctcga caaaaagacc
    94021 gtgaatacct accgctaaag aggctgacca gacaactttc atggcatgag caaccagtgt
    94081 tgctccccat ccctgcccct gtaatgaacg atcaattgcc agacgcccaa gagttacgct
    94141 gggaatattt ttgtagggaa tttttttctg tttcgatttc gagggcagtg cggctcgttc
    94201 aaaacaactg ccggataatg tgtaatatcc cagcacctga cgttctggag tgttgcgaca
    94261 caggatatac gctcgcagaa ttttattgcg atgttgacga accagatgtg tcgtcagaaa
    94321 gagatttaat gcttcctcgc cgcagtcgaa tcgctgtaga tcataatctg catcatcggt
    94381 cagaatctct atcgtcagat catccataaa gtattacatt ccctgaagac gttttgccgc
    94441 acgttttagc ttttcacctg gtgacggtgg attactcagc gcatccatca cccgcgtcca
    94501 ggattcctca gtgagaatca tccgccgatg ctgttcaatc acttccgcag ctcgctgcga
    94561 agcgctgttc aacataaatt ggctgacgga ctgattagat atcgctgccg cttcctcaat
    94621 catacttttg tcatcgtcag ttaaacgcag atcaatacgc tgctttttaa cagcagacat
    94681 gtgaactctc ctgctccgta taatatgtac ggcatatcac cgtacataag catatcctga
    94741 attgttcaat ctttctacaa ccatttcgat aaatataaaa aaacccgatg gcagttcagc
    94801 gcatcgggta ttttcattta tctatctgtt acttcgcatc agtcgccgtg ccgttggcgt
    94861 gccagtcaaa cacaccgaac tcaaagcctt tcagatcgcc tttctcatcc cagctaagcg
    94921 gccccatcac cgtatcaacc gagttcgctt tcaggtattt agcgatctcc gccggatcgt
    94981 cagactgatt cagccccgct tgcaaagatt gcagcgcggc gtaggtggtc cagacgaacg
    95041 cgccgcttgg gtcctgtttt ttcgctttga tcgcgtcaac aatcggtttg ttcgccggaa
    95101 cctgatcgta gttcttcggc ttggtcacca gcagcccttc cgctgattcg cccgcaatgt
    95161 tagacagcga gacgttagcc acgccttctg gccccataaa ctgagttttc agccctgccg
    95221 cgcgtgcctg acgcaggatt tgccccattt ccgggtgata accgccgtag taaacgaaat
    95281 cgatattctc tttcttcaaa cgcgccacca gggtagagaa atctttttcc ccggcggtga
    95341 tgccatcaaa gaacaccacg ttagcattgc ctttcttcag gccgtcctgc actgcacgcg
    95401 ccagaccttc gccgtactgc tgtttgtcgt gaacgatagc aatgcgttgc ggtttcactt
    95461 tctcaagaat atatttcgcc gccgtcggcc cctggtcgga gtccaggccg gtggtgcgca
    95521 ggatcagctg atagccacgg gcggtcagct ctggtgcggt tgccgctggg gtgatcatta
    95581 aaatgccttc gtcttcgtag atgtcagacg ctggctgcgt tgatgaagag cagaggtgac
    95641 caatcacgta tttaatgccg tcgttaacga ctttgttcgc caccgcaacc gcctgtttcg
    95701 ggtcacaggc atcgtcatat tttacgattt gcagtttgtt gcctttgata ccccctttag
    95761 cattgatatc cgcaaccgcc tgctctgcgc cagtaaactc ctggtcaccg tactgcgcca
    95821 ccggaccgga cattgcgcct acgacggcga ctttaatatc ttctgccaga gccatattgc
    95881 tgaatgccag cgcgatacat cctgccagta acgctttacc ctttatgttc atcctgagaa
    95941 tccccactct tctggttatt acgtgtgttg tgatgttgtt gtgcagcact ttatttcgtt
    96001 ttatacgtag ctacccgtgc tttagcagca tactctgcta aaacataccc catttttatg
    96061 atattggaat agctattttg acagtttatt aacaatctgc gtggggattg gcgttttgcc
    96121 ggagggggaa ttgattattg tagaggccca aaaaacaaaa accccggact ctcatccagg
    96181 gttctctgct taatagcgga aattacgctt caatagcagc gcgcaatttt ttcatcgcgt
    96241 tcttttccag ctgacgcaca cgctcagcgg aaacaccgta acggtcagcc agttcctgca
    96301 acgtggactt gttgtcttcg tccagccagc gcgcgcggat gatatcctgg ctacgctcgt
    96361 ccagaccttg catcgcgtcg gtcagacggt ttgccgcctg ctcttcccag ttatcatctt
    96421 cgatgccgtc ggcaaagtta gatgatttat cctgcagata gagcaccgga gccatcggct
    96481 ggctgtcgga atcgtcgtcg gaagacaggt caaaggtcat gtcctgtgcc gccatacgtg
    96541 attccatctc acgtacgtct ttgctggtta cgcccagttc acgggccacc atctcgactt
    96601 catcctggtt aaaccagccc agacgctgct tggttttacg caggttgaag aacagtttgc
    96661 gttgcgcttt ggtggtcgca actttgacga tacgccagtt acgcagaacg tattcgtgga
    96721 tctctgcttt gatccagtga acggcgaagg agaccaggcg cacacccact tccgggttaa
    96781 aacggcgcac tgctttcatc aggccgatgt taccttcctg aatcaaatcc gcctgtggca
    96841 ggccatagcc cgcataatta cgagcaatat gaacaacaaa ccgcaggtga gacaggatca
    96901 gcgttttagc tgcttccaga tcgccatggt aatgcagctt ttcagccagc gcccgctcct
    96961 cgtcagccga caacatcggc cacgcgttag ctgcccggat gtaggaatcc aggttgccaa
    97021 ctggggctaa agctaaactt tgcattttgt cagtcattca aatcctctca atcgatatct
    97081 tctggcgctt cagtggtagc aacaactgtg ccagagctta agagcaacga ggttatcatt
    97141 cactgtttta tcagaccgtg attttatcca caagttcaat gcaagtttgt gaataaatta
    97201 cgcacaaaat gtgacataga gataaaatac cgggaagaga caacggggtc tctttccctg
    97261 ctacggaatc cattgcaggg aaagagtata acacgctttt attattcagg cgtaaagtgg
    97321 cgtaaatgtt gtaccgtggc aagccacgct gccacccagc caatcatcga gcataccagc
    97381 agcaatagca ggcattcatc gaacgataag ccattgatat caaacttcgt tccgaaaacc
    97441 tgtgccactt ccgcaaccgc cgatgacaaa cgcagcacca gaatttctga caaaattagt
    97501 gacaacaatg cgccagaaaa tcccagcaat gcgccaccgt acaggaacgg acgcaggatg
    97561 aaaccatccg tcgcaccaat cagtttctgc acgttaatag aatcacggcg agcaaagata
    97621 ctcaggcgca cactgttacc gatgacgagg aatactgccg ccaccatcaa tacgccgatc
    97681 attgccgaaa cgcgcccgac cagcccggtc aacgccgcca ggcgggcaaa ccagctgtca
    97741 tccatccgca cttcgtcaat gccgttaatc tgcgtgatac gatcacgcag cgtattcagt
    97801 gactccgtcc cctgaaaatc gagtttcggg atcaccaccg ccactgccgg aagcgggttt
    97861 tcttccagca tatccagcgc accaccaaaa ccagaccagt tacggaactc gcccagtgcg
    97921 tcttcacgag aaagataatt cactttctcc acgccttgct cggcctgcaa ctgtgccacc
    97981 acgcccgcag cggcgtcatc atccagcgtt ttttgcagat aaacagtgat ttgcggtgac
    98041 ggataatatt gcgtcgccgc ctggttaacg tttttgtaca ccatataaca gacgctgggc
    98101 agcgtcaggg agatggcgat aaccatcacc gttaaaaacg tggcgaacgg tttgcttttc
    98161 agatcctgca atgcgccgtg ggaggcatag cgtacctgtt cgttgaaaac gttggttttg
    98221 cgatttaccg gttttggcga ggatttcgcg cgtttcggcg cgttacgacc gccgtcgcca
    98281 gagccgccga ccgatttacg gaagcgatcc agacgcccgc caaactgccg aatgtgattg
    98341 attgcatcgc gcttattcat ggcccacgcc tccatgcaag tgaccatcgc tcagggtgag
    98401 catgcgatag gaacgtcgcg aaatcaggtt gatgtcgtgc gttgccatca atacggttac
    98461 tccaacgcgg ttaaactctt caaacagacg taaaatgcct tccgacagcg cgtcgtccag
    98521 gttaccagtc ggttcgtccg ccagcagtac cgcgggcttg ttcaccaccg cgcgggcaat
    98581 gccaacacgc tgttgttcac cgccggaaag ctgaataggg aagttcttcg ctttgtccag
    98641 tagcccgact ttatccagcg ccgccgacac ccggcgacga atgtcatcac cgctggcacc
    98701 ggcgataatc agcgggatcg ccacgttatc atagacagta cggtccatca gtagatggtg
    98761 atcctggaaa atcatgccaa tctggcggcg cagaaacgga acttcacggt ttttcagacg
    98821 cgtgatgtca tggccgctaa accagatttt cccggcgctg ggccgctcaa tcccacagat
    98881 cagcttcagg agggtacttt tccctgcgcc ggaatgaccg gtcagaaacg ccatctcacc
    98941 cggctgcata tggaacgtaa cgccctgcag cgcctgtctc ccaccgagat aagccttgct
    99001 gacatgttca aagcgaatca ttgttaatcc tctcgggcaa aaagtgcctc tataaagtcg
    99061 tccgccttaa acggacgcaa atcctcaata cgttcgccga caccaatgta gcggataggg
    99121 ataccaaact ggtcagccac cgagaaaatt accccgcctt tcgccgtgcc gtccagtttc
    99181 gttagcgtga tgccggttaa gccaacggct tcgtggaaca gtttggcctg gcttaccgca
    99241 ttctgcccgg tgctggcatc gatggtcagc ataacttcat gcggagcttc aacgtcgagt
    99301 ttcttcatca cgcggacgat tttcttcaac tcttccatca ggtgcgattt gttttgcagg
    99361 cgtccggcgg tatcggcaat caggacgtcg atgttacgcg ctttagcggc ctgaatggca
    99421 tcgaagataa cagaagcgga atccgcaccg gtatgctggg caatcaccgg aatattgttg
    99481 cgcagacccc agacctgaag ctgttcaacc gctgccgcac ggaaagtatc gcctgccgcc
    99541 agcatcaccg atttaccctg ctgctcaaac tgacgcgcca gcttaccaat cgtcgtggtt
    99601 ttacccacac cgttgacgcc caccatcagg atcacgaacg gcgttttacc ttcaacattc
    99661 agcggctcat cgactttcgc cagaatctcg cccatctctt ctttcagcag gccatagagc
    99721 gcctcggcat cacgaagctg cttgcgggat gcgccttctg tcagattggt gataatttta
    99781 cgagtggttt ccacgcccac atcggcgatc aacagctgtt cttccagctc ttcaaacaga
    99841 tcatcgtcga tttttttacc gcggaacagg ctgataaatc cggaaccgag attttctttg
    99901 gtttttaaca ggctgcgttt caggcgcgcg aagaaacctt ctttggtcgg tttttcctgc
    99961 tcctgagcga tttcttccac cggctgatct tcttctgccg gagaaaccac cattaccgcc
   100021 tcttctgcca cttcggcagc cagcgcctgt gcctccggct cttcgtcggt gatttcttct
   100081 ttagccgctt cttcttccgc cgcttcgaca atctctacgg tttccgcttc ggcctgccac
   100141 tcttctggcg aaaccgcttc ggcgttgacg tcttccggca acggcagttc ttcacgttcg
   100201 atagccactg gctccggcgt ttcttctacg accggttccg gctgtgcaac gacttccgct
   100261 tcaggctgcg ctttttcact ttcagcaacc tgttcagtaa cttccacaac gtcggcagca
   100321 aaagtttccg cctcggcttc agtatgcgcc tgcggctgct cttcaacggc ttgttcagaa
   100381 gccttcacag gctcttgctc ctgaacgatt tcttctacaa ctggttgttc attctgaact
   100441 tctgtctctt tttccggggt ctgctctttt tgaccaaagc ccagccagga aaaaaagcca
   100501 cgtttttttt ctttcgccat ttgcgactac actcctcgct gttgcttcat ggcacagcgt
   100561 taacgctatg tacatagcag ctaaaaaaat gatgaaatag tctatcactt aacttaattc
   100621 acatcaccgc ctgcaagtat gtgttatctg gcggattgag caatttatca tgaaaaaacc
   100681 gaatcattcc ggcagcggcc aaatccgcat tattggcggg cagtggcgag gccgtaaact
   100741 cccggttcct gatagtccag gtctgcgccc caccaccgac cgcgtacgcg aaacgttgtt
   100801 taactggctg gctccggtca ttgttgacgc ccaatgtctg gattgcttcg ccgggagcgg
   100861 cgcgctgggg ctggaagcgt tatcgcgcta cgctgcgggg gccacgttga ttgagatgga
   100921 tcgtgcggtt tctcagcagt taattaagaa tctggcgaca ctaaaagcag gcaatgcacg
   100981 cgtggtgaac agcaacgcga tgtcattcct ggcgcaaaaa ggcacaccgc ataatatcgt
   101041 gtttgtcgat ccaccgttcc gccgtggctt gttagaagag acgataaatt tactggaaga
   101101 taacggctgg ctggctgacg aagccctgat ttatgtcgaa agcgaagtcg aaaagggtct
   101161 gcccactgtt ccggcaaact ggtcattaca tcgggaaaaa gtggcgggtc aggtggctta
   101221 tcggctgtat caacgcgaag cacaaggaga aagtgatgct gattaatatt ggtcgtttgt
   101281 taatgctctg tgtttgggga tttttaatcc tcaacctggt gcatcccttc ccacgcccgc
   101341 tgaatatttt cgttaacgtg gcgctgattt ttaccgtgct gatgcatggt atgcagctgg
   101401 cgctattgaa atccacttta ccgaaagatg gcccgcagat gaccaccgcc gaaaaggtgc
   101461 ggattttcct tttcggcgtg tttgaactgc tggtctggca gaagaaattt aaagttaaaa
   101521 aataacctta ttgttcgcct acaaagctga caaagcgcgt tcctttatag ctcagcgtac
   101581 ctttatcgcc cactgtcagg gcgtggtact gctgggcgtc gaggcgaaac gtctgctcca
   101641 ttcctccgct ttgcggtttg aagcttgcct catagcgcat actggtgcct gccggcgtca
   101701 cttcctgctg gcgcgaacgg cgatcgttaa tcggtttttc ccgcttgttg ctcaccacca
   101761 ccagcttttg ctggagcggt gccatatcat tatcagcttt ttcccgtcgt tgttgcataa
   101821 aacgaaacga tgcggcgacg atgattaagc caatgataac aataaagaaa agaggtggtt
   101881 tactcatctt tatccctcat cggaaaatgc ggaaataagc ataccctgcc agttatggtg
   101941 ttgtcatccg tccaccctcg ccactaaact ggaagcaaga ccgtaggcat tccgcttacg
   102001 aaaaaataac gaattcaagg aactaagatg ctttggtcgt ttatcgctgt ctgtctttcc
   102061 gcatggctat ctgtggatgc atcgtatcgt gggccaacct ggcaacgctg ggtgtttaaa
   102121 ccgttaaccc ttcttctcct gctgttgctg gcctggcaag cgccgatgtt cgacgccatt
   102181 agctatctgg tgctggcggg gctgtgtgcc tcactgctgg gcgatgcgct aaccctgttg
   102241 ccacgtcaac gtctgatgta cgccatcggc gcgtttttcc tctcgcacct gctgtacacc
   102301 atctatttcg ccagtcagat gacgctctct ttcttctggc ctctgccact ggtgctgctg
   102361 gttctgggcg cgctgttact ggcgattatc tggacgcgcc tggaagagta ccgttggcct
   102421 atctgcacgt ttatcggcat gacgctggtg atggtgtggc tggcaggtga actgtggttc
   102481 ttccgtccga ccgctccggc gctctctgcg tttgttggcg cttcgttgct gtttatcagt
   102541 aactttgtct ggctgggtag tcactatcgc cgacgcttcc gtgcggataa cgcgattgct
   102601 gcggcctgct actttgctgg tcacttcctc atcgtccgct cgctgtatct ctgataaaac
   102661 ttgactctgg agtcgactcc agagtgtatc cttcggttaa tgagaaaaaa cttaaccgga
   102721 ggatgccatg tcgactcctg acaatcacgg caagaaagcc cctcaatttg ctgcgttcaa
   102781 accgctaact acggtacaga acaccaacga ctgttgctgc gacggcgcat gttccagcac
   102841 gccaactctc tctgaaaacg tctccggcac ccgctatagc tggaaagtca gcggtatgga
   102901 ctgcgccgcc tgtgcgcgca aagtggaaaa tgccgtgcgc cagcttgcag gcgtgaatca
   102961 ggtacaggtg ttgttcgcca ccgaaaaact ggtggtggat gccgacaatg acatccgggc
   103021 acaagttgaa tctgcggtgc aaaaagcggg ctattccctg cgcgatgaac aggcctccga
   103081 cgaaccacaa gaatcacgcc tgaaagagaa tctgccgctg attacgctta tcgtgatgat
   103141 ggcaatcagt tgggggctgg agcagttcaa tcatccgttt ggtcaactgg cgtttatcgc
   103201 gaccacgctg gttgggctgt acccaattgc tcgtcaggca ttacggctga tcaaatctgg
   103261 cagctacttc gccattgaaa ccttaatgag cgtagccgct actggggcgc tgtttattgg
   103321 cgcaacggct gaagctgcga tggtgttgct gctgtttttg attggtgaac gactggaagg
   103381 ctgggccgcc agtcgcgcgc gtcaaggggt cagcgcgtta atggcgctga agccagaaac
   103441 cgccacgcgt ctgagtaacg gtgagcgtga agaggtggca ataaacagcc tgcggcctgg
   103501 cgatgtgatt gaagttgcgg caggtgggcg tttgcctgcc gacggtaaac tgctctcacc
   103561 gtttgccagt tttgatgaaa gtgccctgac cggcgaatcc attccggtgg agcgcgcgac
   103621 gggcgataaa gttcctgcag gcgccaccag cgtagaccgt ctggtaacgc tggaagtgct
   103681 gtcagaaccg ggtgccagcg ccattgaccg gattctgaaa ctgattgaag aagccgaaga
   103741 gcgccgcgca cctattgagc ggtttatcga ccgtttcagc cgtatttaca cgccagcgat
   103801 tatggcggtc gccctgctgg taacgctggt gccgccgctg ctgtttgccg ccagctggca
   103861 ggagtggatt tataaagggc tgacgctgct gctgattggt tgcccgtgtg ctttagttat
   103921 ctccacgccc gcggcgatta cctccgggct ggcggcagca gcgcgtcgtg gggcgttgat
   103981 taaaggcggc gcggcgctgg agcagctggg tcgtgttacc caggtggcgt ttgataaaac
   104041 cggtacgctg accgtcggta aaccgcgcgt taccgcgatt catccggcaa cgggtattag
   104101 tgaatctgaa ctgctgacac tggcggcggc ggtcgagcaa ggcgcgacgc atccactggc
   104161 acaggccatt gtgcgcgaag cacaggttgc tgaactcgcc attcctaccg cccaatcaca
   104221 gcgggcgctg gtcgggtctg gcattgaagc gcaggttaac ggtgagcgcg tgttgatatg
   104281 cgctgccgga aaacatcctg ctgatgcatt tgctggtttg attaatgaac tggaaagcgc
   104341 cgggcaaacg gtagtgctgg tagtacgtaa cgatgacgtg ctgggtgtca ttgcgttgca
   104401 ggataccctg cgcgccgatg ctgcaactgc tatcagtgaa ctgaacgcgc tgggcgtcaa
   104461 aggggtgatc cttacaggcg ataatccacg cgcagcagcg gcaatcgccg gggaactggg
   104521 gctggaattt aaagcgggcc tcttgccgga agataaagtc aaagcggtga ccgagctgaa
   104581 tcaacatgcg ccgctggcga tggtcggtga tggtattaac gacgcgccag cgatgaaagc
   104641 tgccgccatc gggattgcaa tgggtagcgg cacagacgtg gcgctggaaa ccgccgatgc
   104701 agcattaacc cataaccacc tgcgcggtct ggtgcaaatg attgaactgg cacgcgccac
   104761 tcacgccaat atccgccaga acatcactat tgcgctgggg ctgaaaggcg ttttcctcgt
   104821 cacgacgctg ttagggatga ccggactgtg gctggcagtg ctggcagata ccggtgcgac
   104881 ggtgctggta acggcgaatg cgttaaggct gttgcgcagg aaataaggca aaccgatcgc
   104941 aacattgagc gcgatcggtc ccctcgctcc tctggggaga gggttagggt gaggggaaaa
   105001 ggcggcatcg aagccaatct tcccccatca accgccttta cgaatcaaat aacgataagg
   105061 cagtccatcc gtctctttag caaccagttc gtgttccata aaggtacaaa acccaggaat
   105121 atcgcgggtc gtcgccggat cgtcagcaat aatcagcaac gtttcacctg gctgcatatt
   105181 gcgcactgtt ttgcgcacca tcattactgg ttccgggcag cgcaggccaa gtgcgtcgag
   105241 tgtgtggtca gggctggaaa agagatcggt cattttcttc tcatcacata aaaaaacggc
   105301 gctagtttac gccctgtgag tccgtaagca aaccaggtta acgattgcgt gaaaattaac
   105361 cattgcattg tcaacgtaaa gcagtatcat gcggcggctc gaaaaaaggg tcagcacgta
   105421 taaaatagcg tgtcgcacgt attgggttcc ctcaccccaa tggttaatca aaaaggtaca
   105481 atatgaacgt tttctcgcaa acgcaacgct ataaggcgtt gttctggtta tcgttatttc
   105541 atcttctggt gatcacctcc agtaactatc tggttcagct tccggtctcc atttttggtt
   105601 ttcataccac ctggggcgcg tttagctttc cgtttatctt tctcgctact gacctgaccg
   105661 tgcgtatttt tggcgcgccg ctggctcgtc gcatcatctt tgcggtaatg atccccgcat
   105721 tgctgatctc ctacgtcatc tcatcgctgt tttatatggg gtcatggcaa ggattcggcg
   105781 cgctcgccca cttcaacctg tttgtcgccc gtatcgccac cgccagtttc atggcctacg
   105841 cgctggggca gatcctcgac gtgcacgttt ttaaccgcct gcgtcagagt cgtcactggt
   105901 ggctggcacc gacagcgtcc acactgttcg gtaacgtcag cgacacgctg gccttcttct
   105961 tcattgcctt ctggcgtagc ccggatgcct ttatggctga acactggatg gaaatcgcgc
   106021 tggtcgatta ctgtttcaaa gtgttaatca gtatcgtttt cttcctgcca atgtatggcg
   106081 tattactcaa tatgctgttg aaaagactgg cagataaatc cgaaatcaac gctttgcagg
   106141 cgagttaaag gttcgttatc agagttgtga taagatggat gaatgagccg ttatggccgt
   106201 ttatcgaaag gaagaagtca atgcgcaatc tggttaaata tgtcggaatt ggcctgctgg
   106261 ttatggggct tgcggcctgt gatgataaag acactaacgc tacggcgcag ggttcggtcg
   106321 cagaaagtaa cgcgaccggg aatcccgtca acctgcttga tggcaagtta agtttctcgc
   106381 tgccagcgga tatgaccgac cagagcggta agctgggaac gcaggccaat aacatgcatg
   106441 tctggtccga cgccaccggg cagaaagcag tcatcgtcat catgggcgat gatccgaaag
   106501 aagatctggc ggtgctggcg aagcgtctgg aagatcagca acgtagccgc gatccgcagc
   106561 tgcaagtggt aaccaataaa gccattgagc tgaaaggtca caaaatgcag cagttagaca
   106621 gcattatctc cgcgaaaggc cagacggcgt actcttccgt tattctgggt aacgtgggta
   106681 atcaactgct aaccatgcaa attacgctgc ccgctgacaa tcagcaaaaa gcacagacca
   106741 ccgcagaaaa catcattaat acgctggtta ttcagtaagt tttaagatga tgaggcggcc
   106801 tcaggtattt ccaccggggg ccgttttttt aatcgccacg tcagtaataa cgcgattgcg
   106861 accagtcccg ccgccgccag ataaattacc ggaacgcccg cccaactcat caccagccca
   106921 gccagtggtc cggtcacgcc aagcgataaa tccataaata cggtgtaagt tgccagcgcc
   106981 gccccctgat tttgctgcgg aaccgctttt accgccacta cgcccaatgc agggaacacc
   107041 agcgaaaacc ccgcccccgc cagtaagacg ccgattttcg ccatccacgg catagtcgcc
   107101 acgccaacca gtagcaggcc gattatctca acgctaaagc aaatcatcgc cacgtttaag
   107161 ccgccgatac ggttaatgcc gttagggaat aacaaacgcg taccgacaaa cgcacagcta
   107221 aacagcgtca gcgcgaaagc cgcaccgtcc caacctttag cgtcataaaa caacgtgata
   107281 aaggtggcga taacgccaaa tccggcggaa gccagtgcca gcgccatacc gtacagccag
   107341 acgcgcccaa gcaccgcacg aaacggcagc ggtttgcctt tactggcttt taccgtcgga
   107401 cgcgggatcg ccaacaaaat ggccaccagt gccacgccca taatgattaa cgctaacgcc
   107461 tgcaatccgc cccagtgata aaacacgaca cctaacggcg cacccatcgc catcgccccg
   107521 taagtgacaa taccgttcca cgaaatcacc cgcccgatat gtagcgaacc aaccacgcca
   107581 acaccccaca gggtcgatcc cgttccggca aaactttgtc caatcccaag gattacgcgc
   107641 cccaggcaca gtaataacag gctgatgacg ggcagactgg cggttaatcc tgccgtcaga
   107701 tatcccagac cgctcataaa gcagccgcat aaaccgaaga cgacaatctt tttgggtccc
   107761 agcaaatcgg cgtaacgtcc ggcatgagga cggctcagca aggtggcgaa atattgcagg
   107821 ctgataacca accctgccca gaaggcacta aagcccatca catcatggac atagcctggt
   107881 aatacagcga gcggcaaccc gatggtgagg tagctggcga agttaaacat gacaatggag
   107941 acaatgcgca aattcaggcg caatccgttt agcgcgggtt cggctacggg ttcgggcatg
   108001 aggatcacca catttttaca acagtgtttc atttttacca tgtgctgacg tgaaaatcag
   108061 cagtaagaat cagaatattg ctggcgtgac tcccgctaca cttaatacaa aaagtcacaa
   108121 ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcgcattc tgctcaagtt
   108181 ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca ttgtgcagct
   108241 attactggcg ctgttttttg ccatcgttct caacccgctc gtcacctggt ttattcgtcg
   108301 gggagtacaa cgccccgttg ccattacgat tgtggttgtg gtgatgctga tcgcactcac
   108361 cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc tgccgaagtt
   108421 taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt ttcttaattt
   108481 gcatatgtcg ccggagcgaa tactacagcg gatggactcg gaaaaagtgg ttaccttcac
   108541 cacggcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc tggtgatgac
   108601 cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt tcgcgttgaa
   108661 taatccacag attcacatcg ccggactaca ccgcgcactt aaaggcgttt cgcattatct
   108721 ggcgttaaag acgctactca gtttatggac aggcgtaatc gtctggctgg ggttggagct
   108781 gatgggggtg cagtttgcgc tgatgtgggc agtactggcg tttttgctca actacgtgcc
   108841 caatatcggt gcggtaattt ccgccgtacc gccaatgatt caggtgctgc tgtttaatgg
   108901 tatttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg tcatcggcaa
   108961 tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg tggtatttct
   109021 ttcattgtta atttggggat ggctgctcgg cccggtaggg atgctacttt cggtgccatt
   109081 aaccagcgtg tgtaaaatct ggatggaaac caccaaaggc ggcagcaaac tggcgatttt
   109141 actggggcca ggcagaccga aaagtcggtt accgggatga ggcgacaagt gttacgatac
   109201 gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc tggggaaagt
   109261 ttcgacctta agcgcagctc ctctgccact gggtttacgc gagcaagcac cgcaaggtcc
   109321 acgacgcgaa cgctggctgg cggggcgtgc attgctttcg cacacgcttt ccccgctacc
   109381 ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc cgctatggtt
   109441 caacttaagc catagcggtg acgatatcgc cctgctgttg agcgatgaag gcgaagtcgg
   109501 ctgcgatatc gaagtgattc gcccgcgcgc caactggcgc tggctggcga acgccgtatt
   109561 cagcctcggg gaacatgccg agatggacgc cgtgcatcct gatcagcaac tggaaatgtt
   109621 ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca gcgcctggca
   109681 aatcgtcagc gtagacagca cctataactc ctcgctgtca gtcagccatt gtcagctaga
   109741 aaatttaagc ctggcgatct gcacccctac cccctttacg ctcaccgccg acagtgtgca
   109801 atggatcgat tcagttaact gatccgccca cccgactgcc catctattga tccagaacag
   109861 gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct attttaatcc
   109921 attggggtta ccatgttctc tacactccgc cgcactctat ttgcgctgct ggcttgtgcg
   109981 tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc ggtgaatgtc
   110041 gggccactaa acccgcacct ttacacgcct aaccaaatgt tcgcccagag catggtttat
   110101 gaaccgctgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc aaaaagctgg
   110161 acccattcgg aagatggcaa aacctggacc ttcaccttgc gtgatgacgt gaaattctcc
   110221 aacggcgaac cgtttgatgc agaggcggcg gcagaaaact tccgtgcagt gctcgataac
   110281 cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgtcaa agcactcagc
   110341 aaaaacgagc tgcaaatcac cctgaaaagc gcctactatc ctttcctgca agaactggcc
   110401 ctgccgcgtc ctttccgctt tattgccccg tcacaattta aaaatcacga aaccatgaac
   110461 gggataaaag cgccgattgg caccggaccg tgggttttgc aggaatcgaa actgaatcag
   110521 tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa aaccggcgat taaaaagatc
   110581 acctttaacg tgatcccgga cccgactacc cgcgcggtgg cgtttgaaac tggcgatatc
   110641 gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg ctttagccag
   110701 aacccggctt accacactca actgtcacag ccgatcgaaa ccgtgatgct ggcgctcaat
   110761 accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta cgcggtgaac
   110821 aaaaaatcgc tgattgataa cgcgttgtat ggtacccagc aggtcgccga caccctgttt
   110881 gccccttctg tgccctacgc caacctcggc ctgaaaccgc gccagtacga tccgcagaaa
   110941 gcgaaagcgt tgctggaaaa agccggttgg acgctgcctg ctggcaaaga catccgcgag
   111001 aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc gttaagcaaa
   111061 tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt ctcgctgatt
   111121 ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg catgattttc
   111181 caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat gcgcgtaccg
   111241 tcgcacgctg acttccaggc acagcaaggt ttagccgaca agccgctgat tgataaagag
   111301 atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta tcgcgacatt
   111361 ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gttacatctc aatgatggtg
   111421 gtttcaaaac cggaactggg taacatcccc tacgcgccga tcgccaccga aatcccgttc
   111481 gaacagatta aaccggtgaa accttaatgt tgcgttatgt attacgccgc tttctgctgc
   111541 tgatcccgat ggtgctcgcc gcctcggtga tcattttttt gatgctgcgc ctcggcaccg
   111601 gcgacccggc gcttgattat ttgcgtttat ctaacctgcc gccgacgccg gagatgctgg
   111661 cctctacccg cactatgctg gggctggatc agccgctgta cgtccagtat ggcacctggc
   111721 tatggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc ccggtgctgg
   111781 acgatatgct gaacttcctg cccgccacgc tggaactggc gggtgcggcg ctggtattaa
   111841 ttctgctcac ctccgtgccg ctcggtatct gggcggcgcg ccaccgcgat cgcttgcccg
   111901 atttcgccgt ccgtttcatc gcgtttctcg gcgtgtcgat gcctaacttc tggctggcgt
   111961 ttttactggt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg ggttacggcg
   112021 gctggcagca catcattttg cctgcggtat ccatcgcctt tatgtcgctg gcgattaacg
   112081 cgcgtttgct gcgcgccagt atgctggacg tcgccggtca gcgtcatgtg acctgggcgc
   112141 ggctgcgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc aatgcctcgc
   112201 tgccgatgat cactgccgtg gggatgcaca ttggcgaact gattggcggg acgatgatta
   112261 tcgaaaacat ctttgcctgg ccgggcgtcg ggcgctatgc ggtgtcggcg atttttaacc
   112321 gtgactatcc ggtgatccag tgctttacgc tgatgatggt ggtggttttt gtggtctgta
   112381 atttgattgt cgatttgctc aacgccgcgc tggacccgcg tattcgtcgt catgaaggag
   112441 cgcacgcgtg aactttttcc tctcctcccg ctggtcggtt cgcctggcgc tgatcattat
   112501 cgccctgctg gcgctgattg cgctcaccag ccagtggtgg ctgccgtatg accctcaggc
   112561 tattgatttg ccgtcgcgcc tgctctcgcc ggatgcgcag cactggctgg gcaccgatca
   112621 tttagggcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc tcggctcggt
   112681 aatggcctgc ctgctgttgg tgctgacatt agggctgatt attggcggca gcgccgggct
   112741 gattggcggg cgcgttgatc aggccaccat gcgcgtcgcg gatatgttta tgaccttccc
   112801 aacctcgatt ctgtcgttct ttatggtcgg cgtgcttggc accgggttga ccaacgtaat
   112861 tatcgctatc gccctttcgc actgggcatg gtacgcgcgc atggtgcgca gtctggtgat
   112921 ttcactgcgc cagcgcgagt ttgtgctggc atcacggctt tctggcgcgg gccatgtgcg
   112981 ggtgtttatt gatcatctgg caggtgcggt gatcccctcg ctgctggtgc tggcgacgct
   113041 ggatatcggt catatgatgc tacacgtcgc ggggatgtcc ttcctcggtc tcggcgtgac
   113101 cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgtcagtata tctggaccca
   113161 gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg cctttaacct
   113221 ggtgggtgac gcactgcgcg atcatctgga ccctcatctg gtgacggagc acgcacacta
   113281 atgccacaac agattgaact ccgtaatatc gcgctacagg ccgcgcagcc gctggtgcac
   113341 ggcgtatcgt taaccctgca acgtgggcgt gtgctggcgt tagtcggcgg tagtggcagc
   113401 gggaagtcgc tgacctgcgc cgcgacgctg ggcattctgc ccgcaggcgt tcgccagacg
   113461 gcgggggaaa ttttggccga tggaaaaccg gtttctccct gcgccctgcg cggcatcaaa
   113521 attgccacca tcatgcagaa cccgcgcagt gcctttaatc cgctgcacac catgcacacc
   113581 cacgcgcgtg aaacctgcct ggcgctgggg aaacccgccg atgacgctac gcttaccgct
   113641 gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctcta cccgttcgag
   113701 atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg cgaatcaccg
   113761 tttatcatcg ccgatgaacc aaccaccgat ctcgacgtgg tagcacaagc acgcatcctc
   113821 gatctactgg aaagcattat gcaaaaacaa gcaccgggaa tgctgctggt tacccatgat
   113881 atgggcgtag tagcgcgtct ggcagacgat gtggcggtaa tgtctcacgg taaaattgtc
   113941 gaacagggcg atgtagaaac gctgtttaac gcccccaaac atgcggtaac gcgcagcctg
   114001 gtttccgcgc atctcgccct ctacggtatg gagctggcat catgacttta cttaacgtct
   114061 ccgacctttc ccatcactat gcgcacggtg gatttagcgg aaaacatcag caccaggcgg
   114121 tgctgaataa cgtttccctg gccctgaaaa gcggtgaaac cgtcgccctg ctgggacgca
   114181 gcggctgcgg aaaaagtacc ctcgcgcggc tgctggtagg gttagagtcg ccctcacagg
   114241 gaaatattag ctggcgcggc gaaccgttgg cgaagctcaa tcgcgcgcaa cgtaaagcgt
   114301 tccgccgtga tattcaaatg gtgtttcagg actccatcag cgccgtgaat ccgcgcaaaa
   114361 ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcgctgaaa aaagccgaac
   114421 aactggcgcg caccagcgaa atgctgaagg cggtcgatct cgacgacagc gtgctcgaca
   114481 aacgcccacc gcagttaagc ggcggccagc tccagcgtgt ctgtctggca cgcgcgctgg
   114541 cggtcgaacc gaaactgctg attctggatg aagccgtttc gaaccttgat ctcgtgttac
   114601 aggcgggcgt cattcgcctg ctgaaaaagc tgcaacaaca gtttggcacc gcctgcctgt
   114661 tcatcaccca cgacttacgc ctggtggaac gcttctgtca gcgggtgatg gtgatggaca
   114721 acggacaaat cgtcgaaacc caggtggtgg gagataaatt aaccttttcc tctgacgccg
   114781 gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc accacagaaa
   114841 aggtttaacg caaatgcaac gagtcaccat cacgcttgat gacgatttac tggagacgct
   114901 ggacagcctg agccagcgtc gtggttataa caaccgttcc gaggcgatcc gcgatattct
   114961 gcgtagcgct ctggcgcagg aagccactca acaacacggc acacaaggtt tcgcggtgct
   115021 gtcgtatgtg tatgaacacg aaaaacgtga cttagccagc cgtattgtct ccacccagca
   115081 tcatcaccac gatctctcgg ttgctactct gcatgtgcac attaatcacg acgactgtct
   115141 ggaaatcgcc gtgttgaaag gtgacatggg tgacgtacaa cattttgccg acgacgtcat
   115201 cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag attaacacct
   115261 cccaacgcgc cgttatttcc tgacggcgca gtagactcct gccaaatatc gactcacgtc
   115321 acattttttt gatcacgatt tggtccccta aaatgtgaga cgcttcgatc ctctttttcg
   115381 cctgttcacc tactatacgc ttgaagttgt ccatgattgt ccatagacaa ctgtaaatgt
   115441 tcaggtgcgc gccatgaaat cattgagtaa gtcgtcacaa ataccgctct atcaacaagt
   115501 ggtggagtgg ataagagaaa gtatttatac cggggatctg gtggaagacg atcgcattcc
   115561 ttcggaatac cagattatgg atatgctgga agtgagtcgg ggaaccgtta aaaaagcggt
   115621 cgcccaactg gtaaaagaag gcgtgttgat tcaggttcag gggaagggaa catttgtcaa
   115681 aaaagagaac gtggcatatc cgttaggtga aggattattg tcattcgcgg aatcgctgga
   115741 aagccagaaa atacatttta ccactgaagt tattacgtca cggattgaac cggctaatcg
   115801 ttatgtggca gagaaattaa gaataacgcc cggtcaggat attctttacc ttgagcgttt
   115861 acgttcaatt ggtgatgaaa aagcgatgct gatagagaac cgtatcaata ttgagctatg
   115921 ccccggcatc gtggaaatcg attttaatca acacaattta tttccaacaa tagaaagttt
   115981 gtcgcaaaga aaaattcgtt actcggaaag tcgctatgcc gcgcgattaa ttggtaatga
   116041 acgcggtcat tttttagata tcagtgaaga tgcacccgtt ttgcatctgg agcagttagt
   116101 ctttttctcc cgcggattac ccgttgagtt tggcaacgtc tggttaaaag gaaataaata
   116161 ctatcttggc accgtgctgc aacggcggga agtgagttaa gtacactttt ttaagaggaa
   116221 taaaatgcaa ggcatacagt ttcaggaaaa ttttattcaa cgacttccgg cagggttaag
   116281 cgttgaacag atcatccgtc agttagcgca accgttggtt accgccgaac tggtggtccc
   116341 tgattttgcc gatcatgtac tggaacgtga ggcgacgtac ccaacgggac tgcctaccga
   116401 accaccgtgt gtcgccattc cgcacacgga ccataaacat gtcaggcaca atgcaattgc
   116461 cgtcggcatc ctgccggaac cggtagtgtt tgccgatatg ggcggcgacc ctgatcccgt
   116521 acctgtcagg gtgatttttt tgctcgcctt aggcgaaagt aacaaacaat taaatgccct
   116581 ggggtggatt atggagatga ttcaggatac gccctttatg tgcgccctgc ttacgatgga
   116641 aacaacagaa atacacacag caatcttaaa caaaatgaaa gaacgaggtg aaatatgagt
   116701 caaaccattt tatttgtctg cgccacgggt attgccacat ccacggcagt aacagaaaaa
   116761 gtcatggaat attgtaaaga gcacggtctt aacgttaatt attctcaaac caacgttgcc
   116821 tctttacctg gaaataccga cggcgtagca ctggttgttt caacgactaa agtgccttat
   116881 gaactcgacg tgccggtggt taacggcttg ccaattatta ctggtattgg cgaagagaaa
   116941 gtattggcgc aaattgtttc tatcctaaaa aagtaattaa taataattaa tttcaacaca
   117001 ctacatcagg cgctaacgcg aggtgcgctc tcgcctgaaa aagaggttta ttatgaacga
   117061 tatcgcgcat accctctata atattgtgca atatatattg ggatttggtc ccacggtaat
   117121 gttgccgttg gtgttattta ttcttgctct ctttttcaaa gtaaaacccg ctaaagcctt
   117181 acgttcgtca ttaacggtcg gcattggttt tgtcggtatt tatgccattt tcgatattct
   117241 taccagcaat gtcgggccag cggcccaggc gatggttgaa cgcaccggaa ttaatttacc
   117301 ggtggtggat ttaggctggc cgccgctttc cgctattaca tggggttcgc caattgcccc
   117361 gtttgttatt cccctgacta ttctgattaa cgtggcgatg ctggcgttaa ataaaacccg
   117421 caccgttgac gtagatatgt ggaactactg gcattttgcc cttgccggta cgctggttta
   117481 ttacagctcc ggcagcctgt tctttggttt gctggcggcg gcgattgctg cggtggtagt
   117541 acttaaactc gccgactggt ctgcgccact ggtacaaaaa tactttggcc tggaagggat
   117601 ctctttgccg acgctctctt cggtggtgtt ctttccggtc ggtctgttgg tcgacaaaat
   117661 catcgaccat atccccggcc tcaatcgtat tcatatcgat ccggaaaccg tacagaaaaa
   117721 gtttggcatt ttcggcgaac cgatgatggt tggcactatt ctgggcattt ttctcggcgt
   117781 aattgccgga tacgatttca aaaaagtatt gctgcttggc atcagcattg gcggtgtgat
   117841 gttcatcctg ccacgcatgg ttcgcattct gatggaaggt ttattaccgc tgtctgaagc
   117901 cattaaaaag tatctcaatg ccaaataccc tgaccgtgac gatctctata tcggcctgga
   117961 tatcgccgtt gccgtaggta atccggcgat tatctccacc gccctgctgc taacgccaat
   118021 ctcggtcttt atcgcgtttg tcctcccagg taatgaagtc ctgccgcttg gcgaccttgc
   118081 caacctggcg gtaatggcgt cgatgattgc tttagccagc cgtggcaaca ttttccgcac
   118141 cgttctggcg gcgatcccgg tgattattgc cgacctgtgg attgctacca aaatcgcgcc
   118201 gtttattacc ggaatggcga aagacgttaa cttcaaattt gccgaaggct ccagcggcca
   118261 ggtttccagt ttccttgatg gcggtaaccc gttccgcttc tggctgctgg aaatcttcaa
   118321 cggcaatctc atcgccattg ctctggtgcc ggttattgcc ctggtgctgt atggcatttt
   118381 ccgaatgacg cggagcacgg tttatgcctg ataacagcgc cgctatcgtt atcgatattg
   118441 gcaccaccaa ttgcaaagtc acctgctttt cctgcctgga cgcaacgacg ttgggcgcgc
   118501 ataaattcgt gacggcaaaa cagatctccc cacagggcga tgtcgatttc gatatcgacg
   118561 ccctctggca ggaggtccgc caggcgatag cgcaactgaa cgccgcttca ccgctgcctg
   118621 tcaggcggat cagcattgcc agttttggcg agtcaggggt gtttcttgac gaacatggcg
   118681 agatcctgac gccaatgctg gcatggtatg accgtcgcgg tgaagagtat ctggcaacgc
   118741 ttagcgaggc agacagtgcg gcactttatg acatttgcgg attaccactg cacagcaatt
   118801 actctgcctt caaaatgcgt tggttgctgg agcattatcc gctgcgcaat cgtcgcggcc
   118861 tgcgctggct acatgcaccg gaagtgctgc tctggcggct gactggcgaa cagcgaacgg
   118921 atatcacctt agccagccgt acactgtgtc tggacgtgcg caaaggcgaa tggtcagcga
   118981 aagcgacggc gttgttacac gttccctgtt cagcatttgc gccattggtg cagccaggcg
   119041 aacacgccgg atgggtcagc gagtcgctct gcaaaacgct tgggttctcg caaccggtca
   119101 gcgtgacgct ggccggacat gaccatatgg tgggtgcgcg ggcgttgcag atgatgccgg
   119161 gcgatatcct taactcgacg ggcaccacgg aaggcattct gcaactggat acacaaccga
   119221 cgctggatga acaggccaaa cgtgacaagc tggcaaacgg ctgttactcg cttgccaacc
   119281 agttcaccct gtttgcttct ctgcccgtgg gcggtttcgc gctggagtgg ctgcgcaaca
   119341 cgttccggct aactgatgag gagatcgccg cgtcgctcaa tcgtgggtat gcggattacc
   119401 tggcggggaa ctggtcgctt gatgacattc ccgtctttat tccgcatctt cgcggttcgg
   119461 gttcacccta taaaaatcgc cacacccgcg gattatttta tgggcttggc gatacgttaa
   119521 atatcgacat gttaatttcc agcgtctcac tgggattaac catggaattt gccaactgct
   119581 ttgcctgttt taatgtgcct ggcaccagcg tgttaaaagt gatcggtccg gcgacccata
   119641 accccctttg gctgcaatta aaggcagata ttttacagcg tccggttgaa gcaattgcat
   119701 ttagcgaggc ggtttctgtc ggagccttat taaccgcggc accggatatt ccgccgccgc
   119761 aagttaccat agcccaacgt ttgttaccga atcgggcaag atatcatcaa ttacagcgtt
   119821 atcagcacaa atggaaaagc tggtatcagt tgaaattaca gcaagaaggc gtgatgccat
   119881 tacatcatcg ggaggagcac tatgttgact aaaaccgtgg aggtcagaaa cagtactggc
   119941 ctgcatgcac gtccggcggc ctgtctggcg aaagccgcca aaaaatgtag ctgtaaagtg
   120001 acgctgcact atgagggtaa tgatattaat gccaccagta tgatgaatat tatgcgcgcc
   120061 ggaataaaag gcggaaaaac ggtggaaata cgctgcgacg gtgatgatga aaacgaggcc
   120121 atacagactc tcaccgcctt atttcgcgac cgcttcggtg aagctgaata attaactgat
   120181 attaaaaaat gaggttttca atatgccatt agtgaatgga cggatactgc tcgaccgcat
   120241 tcaggaaaaa cgggtattag cgggcgcgtt taataccacc aatctggaga cgacaatttc
   120301 aattttaaac gctattgagc gttcgggatt acccaacttc attcagattg cgcccaccaa
   120361 tgcacaactg tcgggatacg attacatata tgaaatcgtg aaacgtcacg ccgataaaat
   120421 ggatgttccg gtcagtcttc atctggatca cgggaaaacc caggaggatg ttaaacaggc
   120481 ggtgcgcgcg ggctttacct cggtcatgat tgacggtgcc gcattctctt ttgaagagaa
   120541 tatcgctttc acccaggaag ctgtcgattt ctgtaaatct tatggcgtgc cggttgaagc
   120601 ggagctgggg gcgattctcg gcaaagaaga tgatcacgtc agcgaggccg attgcaaaac
   120661 ggaaccggaa aaggtgaaga cctttgtcga acgcaccggt tgcgacatgc tggcagtttc
   120721 tattggtaac gttcatggcc tggacgatat accgcggatc gatatccccc tgctgaagcg
   120781 cattgccgaa gtcagcccgg tgccgctggt tatccacggc ggctccggta tcgccccgga
   120841 gatattacgc agtttcgtta attatcgcgt cgctaaagtg aatatcgcca gcgatctgcg
   120901 caaagcgttt atcaccgcag tcggcaaagc gtatgtgaat aatcacaacg aggcgaatct
   120961 ggcacgagtg atggcatcag caaaaaacgc cgtcgaggaa gatgtgtata gcaaaatcct
   121021 gatgatgaat gaggggcatc ggtcggtaaa ataaatcctc gccggatgcg gcgtgacgcc
   121081 ttatccggcc tacaaaacca ctagaattca ataaattgca ccatcgcgta ggcccgataa
   121141 gcttgcgtat cgggcactgt atttacgcca ttgtcccaat cgtcttcctg aatcgcagca
   121201 gcgcaatggt aaaaaacgcg ccgccaatcg ctatcagcgt caggaactgc ggccagacga
   121261 tttcgaatcc ggcaccacga tagaggatgg cctgcgcgag gctgacaaag tgtgtcgtcg
   121321 gcatggtcag cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggaaccac
   121381 cggaaagcat ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg
   121441 aacgcgctat cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca
   121501 cgcccagcat aaacagcggg atcgagcctt ctatcggtac gcccagcacg cctttcacca
   121561 tcagcaccag cgataatccc gacaccacca gcaccaccag ccccatcgac cagacctttg
   121621 ccatcatgat ctcaaacggc gttatcggca tcaccagtaa gtgttccacc gtgccgtgtt
   121681 cacgctcgcg gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga
   121741 tgatcgccat caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccgtg
   121801 tttccagcga taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt
   121861 caccgttgat aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg
   121921 catcaacgtt cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag
   121981 gcggaatatt tatcgcgaag gtataacgtc cggcgtccag cccggcatcc atctcatcgg
   122041 cggtgatcat ctccggtggc aaaaaccacg ggcgatagaa gctgttaacg atccggttcg
   122101 ataactgcga ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg
   122161 taacggtcgc tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg
   122221 ctttatcacc gagcagactg cgcaactctt tgatacccag attaaaaata ttacgtaaat
   122281 ggcgcatcat ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcggtat
   122341 ggctatcagc aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa
   122401 cgttccgcgg gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc
   122461 tggaccttcc agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa
   122521 cgtgataatt gccgtaccga aaatggcggc aatctggctt ttcataaagg tggagatcag
   122581 cagccccatt ccggtggcga tgatgatata cagcagcgcc gccagggtga gcgtcaggaa
   122641 actgccttta tgcggtacgc caaacacaaa caccgacagg gcgcagagca ggaaaaagtt
   122701 cagcatcccc agcgcgatgt atggcagctg tttgccaagt aaaaattcgc tacgggtggt
   122761 gggggtcacg taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc
   122821 gcttagcatt gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgccgg
   122881 caggcttttt acgtccgggt tatagcgata gcgcgtctca atattcatca gcccgctttg
   122941 gctggcgggt gtggactgtc ggctcgccac atcctgtagc cagctctggt gcatggcctg
   123001 cacgtagcct tttaccgttt cggcgcggct cggcatcgca ccgtcgatcc agacaccgag
   123061 ttccacgggc gtaccacgcg cgatatcgcg cccgaaattg ggagggatct caattgccac
   123121 cgtgatatcg cccgcacgca tccgacgatc aagctcgtca taactggtga gcggtggctg
   123181 ttcgataaag taacgggaac cggagagatt gagcgtccac gcctggctac tgacggtttg
   123241 atcgcggtca agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat
   123301 tatcagcatc aggatcaccg ttcccatcag cgccagcgtc gaacgtaccg gatcgcgtcg
   123361 cagttccagc gcttcgcggc ggctgtagct aaacagacgg cgcaggctga atccctgacg
   123421 tggcgcgtgg gtagtgtcat gtaccacagg cggcgcttcg gcttcgttgc tctgccctgc
   123481 cgcttcctgc aaataagcga taaatgcctc ttcgagactg gcggctccgc gtttctcaac
   123541 caactcctgc ggtgtaccgc tggcgagcac ttttccggcg tgcatcagtg agatacggtc
   123601 acaacgttcc gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg
   123661 cgagagatcc accatcaact gccagaacat atccctcgcc accggatcga cgccagaagt
   123721 cggctcatcg aggattagca tctccgggcg atgaatcacc gccaccgcca gcgatagccg
   123781 ctggcgaatg ccgagcggca atgactccgg caatacatct tcgacgtcgt tgagcttaaa
   123841 acgctcgctc atttcagcca ctcgcgcggg aatttccgct tccgggatat gaaacagacg
   123901 agcatgtaac tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga
   123961 catatagccc acacgacggc gggtatcgat atcttttgga tcaaccggct gcccgaacag
   124021 ccacgcctca ccttcgctgg cgggcagcaa tccggtaagc attttcatgg tggtggattt
   124081 accgcagcca ttcgaaccaa gaaaaccaaa aatctccccg cgtggaatgc ggaaattgac
   124141 gtgatcaacg gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat
   124201 ctctgcgttt tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgtgc
   124261 ttgcggtaac agatttataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcaa
   124321 ctcttcggcg ctaccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa
   124381 gcgttcggcc tcttccatgt aggcggtggc aaccagcacg ctcatattgc tctgccgctg
   124441 gcgaatactg tcgatcagat cccagaactg ggcgcgagag agcgggtcaa cccccgttgt
   124501 tggctcatca agaatcaaca attccgggtc gtggattaac gcacagcaca gcccaagttt
   124561 ttgcttcatc ccgccggaga gtttccctgc cggacgatcg cgaaacggtg ctaatccggt
   124621 gctggtcagc agctcattaa ttcgcacttc ccgctccgct ttgtcgtgac cgaacaggcg
   124681 ggcgaaaaaa tcgacgtttt catacaccga caaggtgtgg tagagatttt tgcccagccc
   124741 ctgcggcatc caggcgatgc gcgggcagac gtcgcggcga tgcttcgggt cgcgcatatc
   124801 gccgcccagc accatcacgc cgccctgctc aatgacgcga gcaccggaaa tcaacgacaa
   124861 caagctcgat ttaccgacac catccggtcc aatcagcccg accatgcaac gggccggaat
   124921 atcgagtgtg atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag
   124981 ttgcgcgaca ggcgggacgg gaaccagttc cagatgcatc attgcggcaa cctcaccacc
   125041 aggtcgtcag gccacggaag ttcttcattc acccgcaccc acgctacgcc cggcaatccg
   125101 gttttgacat attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacacgg
   125161 aacatcaatt tcagccgctc atcgctggtt tcgacagttt ttggcgtgaa ctgggcgacg
   125221 ctggcgacaa aactgatggt cgcgggaata cgcagatctg gtgcagcatc aagaatcaaa
   125281 cgtgcttcac cgcccagttt cagcgtgccc gcctgttcgg ttggcagaaa gaaggtcata
   125341 tagacgtcgc tgagatcgac catattcagc acccgaccgc ctgctgccag cacttcgcct
   125401 ggctcggcaa cccgatactg cacgcgtccg tcacgcgggg ctttcagttc gctgtcatcg
   125461 atatctgcgg caatgcgccg ttcagtggct tgtgccgctt cgacgcgagt ttgcgcctga
   125521 atgatattgg tgcgcgccgc ctctatagcg gctttagaag cagaaacctg agcttttgcc
   125581 gattccagcg cagctcgggc gctctcagcg gcggcgcggt cgtcatccaa ctgttgcgca
   125641 gaaattgccc cccgttgtgc cagtgagcgg gaacgcgtat gacgttttgc tacggagtcc
   125701 agttctgcct ggcgttgatt aaccagcgac tgtgcggcac gagtttcgct ttggcgttgc
   125761 tccagcaaag cctgcgcggc ggcaacggca ctttgtgctt ctttgatttg cgcgatggct
   125821 tccagtcgct gttcctgcaa cacgcgagta tccatcttcg ccagcacttc accttctcga
   125881 acaaactggc cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata
   125941 tccacttccg tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc
   126001 gggcgcaaca gccaccacgc cacgacggca gccaccgcca gtgccccgac accccaccac
   126061 gccagatggc gcttactctt atccataatc gacccgccat aatccctgta agcaatcgac
   126121 acgtgttaca actgccaccg aacgacgttc agcggattct tccatccggg ataagaagga
   126181 aatggcaaat acgaccagcg cacagagcat tgagcgccaa tggggaaggc ttagtgaaaa
   126241 cgggtagtaa tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata
   126301 agtgaaatca attaaatact aacagttata ttcatgttgt ctatttgatt gccatcaact
   126361 tgtgatacat cgcgatgttc ttccgttcgc tggtgttttt tccaaaaata ttctgtacag
   126421 gttcacagat tctgttgcac aggtttccgt aacagggaaa cctgatgact atcacatagc
   126481 gttattatcg tctgatgtgc gaataaatac cgaatataaa ataatttatt ctgaaaagaa
   126541 tgatagatga taaaggctcg tgggtttaat aaaatagtcc ctaaaagatt catttaaaac
   126601 aacaaaataa gataaagata ttaattcaga gtttatctta ggataaatat ttatttaata
   126661 gcattaactc accgctaaaa gttgatcccc ataacgctaa atccgattat agtgaagtta
   126721 ctttttttaa ttgatggcat cttatagaca tatttaacta actgaaaaga cgaatcatat
   126781 gtttttcgaa gtgtagcaga aaatatatat ggggtttaaa atgaatatat atatcggatg
   126841 gttgtttaaa ttaattcctt tactgatggg tataatatgc atcgcattag gtgagtttgt
   126901 attaaccggt tctgggcaaa gtgaatattt cgttgcaggg catgttctga tttctctttc
   126961 tgcgatatgt ctggcattgt tcactaccgc atttattatt atatctcagt taactcacgg
   127021 catgaataaa ttctacaaca agttatttcc tgttattggt tatgccggtt ctgctacaac
   127081 tatgatttgg ggatggtcac tgttggcaag caataatgtg atggcagatg aatttgtcgc
   127141 cggtcatgtt attttcggcg ttggcatgat tgccgcttgt gtatcaaccg tagcggcatc
   127201 ttccggacat tttctgttga tccccaaaaa cgcctcgggc agtaagagtg acggtacgcc
   127261 attgcaggcc tattcttcaa caatcggtaa ttgtttaatt gccgttcccg ttctgcttac
   127321 tctctttggt ttcatttggt cagtcatact gttacgtagt gcagatatca caccacatta
   127381 tgttgcaggt cacgtactaa tggggttaac ggcaatttgc gcttgtctta ttggccttgt
   127441 cgccactatt gtccatcaaa cgcggaatac gttctcagtg aaagaacatt ggctgtggtg
   127501 ctattgggtt attttgcttg gctcacttac cattattttt ggtatctacg tattaatcag
   127561 ttcagatgca agcgcccgcc tggcccccgg cattattctc atttgcctgg ggatgatctg
   127621 ctacagtatt ttctccaaag tgtggttgtt agcgctggta tggcgacgca cctgttcgtt
   127681 agccaacaga atacccatga ttccggtatt tacctgtctg ttttgtctgt tccttgctgc
   127741 gttcctggct gaaattgcgc aaaccgatat ggcttacttt attccttcgc gagttctggt
   127801 tggcttaggt gcagtttgtt ttacactgtt ttccatcgtt tctatattag aagcgggttc
   127861 agcgaaaaaa taataaaaaa ggcttcagat aaggcgttga gcgccttatc tggttttatt
   127921 caggttaatc cctctggtat cgagagcgca ttttgcttct tattctgcca ccaaatcctg
   127981 cgcacaagcc catgcactcg accacgccca ctggaagtta tagcccccca gccagccggt
   128041 gacgtccatc acttcgccga tgaagtacag cccaggcact ttgcgcgctt ccatcgtccg
   128101 tgaagagagt tcgttggtgt ccacgccgcc gagcgtcact tcggcagtgc gatagccttc
   128161 agtgccgttg ggttgtacgc gccagtcagt caatgtgcta atcagtgtct gttggtcacg
   128221 cacgttgagc tgttttagcg agacatccgg gatttgcccg agttgttgta accgttcaac
   128281 caaccgcttc ggcagatgaa ccgccagcgt gtttttcagg ctttgattcg gatgtgcatt
   128341 acgctgctca ttcaggaagg tttcgaggtc cacatccggt agcagattga tgctgacaaa
   128401 ttcacccggt tgccagtagc tggaaatctg caacaccgcc ggtccagata agccgcggtg
   128461 ggtgaagagt aagttctcac ggaaaacggt accgttttca gcggtaatca cggaaggcac
   128521 agccacgccc gccagcacct gtaactcttc aagcaacggt ttatgcagag tgaatggcac
   128581 cagacccgca cgggtcggca gcacgttgag gccgaattgt tcggcaatct tataaccaaa
   128641 cggcgatgcg cccagccccg gcattgacag tccaccggtc gcaatgacca gcttttcgca
   128701 accgacagtc atgccgttca gttcaagcgt gaagcctgta tcatccttcg ccacgctcag
   128761 cacttcgcta cgcaatctga aggccacatt gcccttctcg cactcatcca ccagcatgtc
   128821 gacaatctgc tgcgcggagt catcgcagaa gagctgccct aacgttttct cgtgccaggc
   128881 gatgccgtgt ttattgacca gatcaatgaa atcccactgg gtaaaacgcg cgagtgcaga
   128941 cttacaaaaa tgcggattct ggctcagata agcgcctggt tcgacataaa ggttggtaaa
   129001 gttgcagcgc ccaccgccag acatgaggat tttgcgccct ggttttttac cattatcgat
   129061 cagcagaacc cggcgtcctg cctgacctgc cagcgcagaa cagaacatac ctgccgcacc
   129121 agcgcctata ataatggcat caaacctttc cacgttgcgc tcctctaaga aaaaacgggc
   129181 gtgaattgta aagattcctc agtggtcgca ccagcatcaa tattactaaa aggaagtatt
   129241 tgcctgaatt atataagata attatttttt gagtggaatc catacaggag gcaaatcaaa
   129301 aaaagtctat atttcacttt gcccgcgccg cgaaagtcac tgataatgcg ccgcgttcat
   129361 gtcctcaaaa tggcgtaacg tcctatgcta catttgtttg ctggcctgga tttgcatacc
   129421 gggctgttat tattgcttgc actggctttt gtgctgttct acgaagccat caatggtttc
   129481 catgacacag ccaacgccgt ggcaaccgtt atctataccc gcgcgatgcg ttctcagctc
   129541 gccgtggtta tggcggcggt attcaacttt ttgggtgttt tgctgggtgg tctgagtgtt
   129601 gcctatgcca ttgtgcatat gctgccgacg gatctgctgc ttaatatggg atcgtctcat
   129661 ggccttgcca tggtgttctc tatgttgctg gcggcgatta tctggaacct gggtacctgg
   129721 tactttggtt tacctgcatc cagctctcat acgctgattg gcgcgatcat cgggattggt
   129781 ttaaccaatg cgttgatgac cgggacgtca gtggtggatg cactcaatat cccgaaagta
   129841 ttaagtattt tcggttctct gatcgtttcc cctattgtcg gcctggtgtt tgctggcggt
   129901 ctgattttct tgctgcgtcg ctactggagc ggcaccaaga aacgcgcccg tatccacctg
   129961 accccagcgg agcgtgaaaa gaaagacggc aagaaaaagc cgccgttctg gacgcgtatt
   130021 gcgctgatcc tttccgctat cggcgtggcg ttttcgcacg gcgcgaacga tggtcagaaa
   130081 ggcattggtc tggttatgtt ggtattgatt ggcgtcgcac cagcaggctt cgtggtgaac
   130141 atgaatgcca ctggctacga aatcacccgt acccgtgatg ccatcaacaa cgtcgaagct
   130201 tactttgagc agcatcctgc actgctgaaa caagcgaccg gtgctgatca gttagtaccg
   130261 gctccggaag ctggcgcaac gcaacccgcg gagttccatt gccatccgtc gaataccatt
   130321 aacgcgctca accgcctgaa aggcatgttg actaccgatg tggaaagcta cgacaagctg
   130381 tcgcttgatc aacgtagcca gatgcgccgc attatgctgt gcgtttctga cactatcgat
   130441 aaagtggtga aaatgcctgg cgtgagtgct gacgatcagc gcctgttgaa gaaactgaag
   130501 tccgacatgc ttagcaccat cgagtatgcg ccggtgtgga tcatcatggc ggtcgcgctg
   130561 gcgttaggta tcggtacgat gattggctgg cgtcgtgtgg caacgactat cggtgagaaa
   130621 atcggtaaga aaggcatgac ctacgctcag gggatgtctg cccagatgac ggcggctgtg
   130681 tctatcggcc tggcaagtta taccgggatg ccggtttcca ctactcacgt actctcctct
   130741 tctgtcgcgg ggacgatggt ggtagatggc ggcggcttac agcgtaaaac cgtaaccagt
   130801 attctgatgg cctgggtgtt tacccttccg gctgcggtac tgctttccgg cgggctgtac
   130861 tggctctcct tgcagttcct gtaattgtac gcaccaaaac gagcgggtca gtttactggc
   130921 ccgctttttt tatgcgtcat tattgctgaa gtgcttaatg ccaaatcatc aatgcaatca
   130981 ggctgaccac caccagacca cacaatgcgc tggtcagaat aaactgccga cgcacccgct
   131041 cacagcggcg aataaactca tcatcgtgat gatcgcgata acgttgggca tagatatacc
   131101 aaacgagacg cacctgtttg tttggttggc catgtgaggt aaaaaagccc cctccatcaa
   131161 catattgata gagcaatgga tcgcagttac gcagtaccac taacaacgcg cgtagtgatg
   131221 agaaatagcg cgccatgtta acaatgcaaa cgacacataa agcccaaaat aatgcgacgg
   131281 tgcttatcat acctcctccc cggcgacctg cccgcggagt tccaccccgg ggctaccgct
   131341 cccgatacgc tgccaatcag ttaacaccag gtcctggaga aaccgctttt gtggtgacca
   131401 acatccgagc ggctctatag atagtgtagg agatcaggtt gttttttttc cagaaggtta
   131461 accactatca atatattcat gtcgaaaatt tgtttatcta acgagtaagc aaggcggatt
   131521 gacggatcat ccgggtcgct ataaggtaag gatggtctta acactgaatc tttacggctg
   131581 ggttagcccc gcgcacgtag ttcgcaggac gcgggtgacg taacggcaca agaaacgcta
   131641 gctggccagt catcgacaac tttatggaag gagtaacact atggcttata aacacattct
   131701 catcgcggtc gacctctccc cggaaagcaa agttctggta gagaaagcag tctctatggc
   131761 tcgcccctac aatgcgaaag tttctctgat ccacgtagat gtaaactact ctgacctata
   131821 caccgggctt atcgatgtga atctgggtga tatgcagaaa cgcatctctg aagagacaca
   131881 tcatgcgctg accgagcttt caaccaatgc aggctaccca atcactgaaa ccctgagcgg
   131941 cagcggcgat ctgggccagg tactggtcga tgcaatcaag aaatacgata tggatctggt
   132001 ggtttgtggt caccaccagg acttctggag caaactgatg tcttccgcac gtcagctgat
   132061 caacaccgtt cacgttgata tgctgattgt tccgctgcgc gacgaagaag aataatcttc
   132121 cctccacgac gtgttcctga acgcccgcat atgcgggcgt tttgcttttt ggcgcgcctt
   132181 gttacctgat gcagcgtaaa cgccttatct ggcctgcggt ctgcgtacgc aattaaaacc
   132241 cctgccaata caacatttaa caccatcata ttttctatca ttagtgtgat cagctggtta
   132301 ttttctgttg taatagtgta ttaatctatt cgccgcatca atattaagaa tctctgacag
   132361 atgtaaactt tttcgcgcgt tatcccttac gcgttcatac ttttcaggat ggtattggaa
   132421 ggttaataaa tatgaataca acaacaccca tggggatgct gcagcaacct cgcccatttt
   132481 tcatgatctt ttttgtcgag ttatgggagc gattcggcta ctacggcgtg cagggcgtac
   132541 tggcggtttt cttcgttaaa cagcttgggt tctcgcaaga gcaggctttt gtcacttttg
   132601 gtgcttttgc tgcgctggtc tatggcctca tttccattgg tggctatgtt ggcgaccacc
   132661 tgctgggaac caaacgcacc attgttctcg gagcacttgt gctggcgatt ggctacttca
   132721 tgaccggcat gtcgctactt aagcctgacc tgattttcat cgccctgggg actatcgctg
   132781 tcggtaacgg cctgtttaaa gctaacccag ccagcttgct ttcgaaatgc tatccgccga
   132841 aagatccacg gcttgatggc gcattcaccc tgttctatat gtcgatcaat atcggctcgt
   132901 tgatagcgtt atcgctggcc cctgtgatcg ctgataaatt cggctattca gtcacctaca
   132961 acctgtgcgg tgcagggtta attatcgcat tactggttta catcgcctgt cgtggaatgg
   133021 tgaaagatat tggttctgaa cccgacttcc ggccgatgag cttcagtaaa ctgttgtacg
   133081 tattacttgg cagcgtggtg atgatcttcg tctgcgcctg gctgatgcac aacgtagaag
   133141 tcgccaatct ggtgctgatt gttctctcca tcgtcgtcac catcatcttc tttcgtcagg
   133201 cattcaagct ggataaaacc gggcgcaata aaatgtttgt cgcctttgtc ctgatgctcg
   133261 aagcggtggt gttttacatt ctctacgccc agatgccaac gtcgctgaac ttctttgcca
   133321 tcaacaacgt gcatcatgaa attctcggct tttccatcaa cccggtcagc ttccaggcgc
   133381 ttaacccgtt ctgggtggta ctcgccagcc caatactggc aggcatttac acgcaactgg
   133441 gtaacaaagg caaagatctc tcgatgccga tgaagtttac tctcggcatg tttatgtgct
   133501 cactgggctt tttgacggct gcagcggctg gaatgtggtt tgcggatgcc caagggctga
   133561 catcgccatg gtttatcgtg ctggtgtact tattccagag cttaggtgaa ctgtttatta
   133621 gcgcccttgg cctggcgatg attgctgccc tggtgccgca gcatttgatg ggctttattc
   133681 tcgggatgtg gttcctgacg caggctgccg cgttcttgct gggcggctat gtggcaacat
   133741 ttaccgcagt accagacaac attaccgatc cacttgagac gttgcccgtc tataccaacg
   133801 tgtttggtaa gattggcctg gttacgctgg gcgttgcagt agtgatgcta ttgatggtgc
   133861 cgtggctgaa acgcatgatt gcgacgccgg aaagccat
//
LOCUS       DAOFSV010000002        88951 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.002, whole genome shotgun sequence.
ACCESSION   DAOFSV010000002 DAOFSV010000000
VERSION     DAOFSV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 88951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.002"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..301
                     /locus_tag="QBH27_000124"
     CDS             <1..301
                     /locus_tag="QBH27_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDQ7012503.1"
                     /translation="ALLYIMLTYWLIDSFFAFNKYDWMLESGDNICSIPSVSGEDRIL
                     QAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLGCGWWLFWGRFIFCY"
     gene            complement(347..1696)
                     /gene="qseC"
                     /locus_tag="QBH27_000125"
     CDS             complement(347..1696)
                     /gene="qseC"
                     /locus_tag="QBH27_000125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDQ7012504.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAKQGGFEAKVSW"
     gene            complement(1693..2352)
                     /gene="qseB"
                     /locus_tag="QBH27_000126"
     CDS             complement(1693..2352)
                     /gene="qseB"
                     /locus_tag="QBH27_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDQ7012505.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            2504..2896
                     /gene="ygiW"
                     /locus_tag="QBH27_000127"
     CDS             2504..2896
                     /gene="ygiW"
                     /locus_tag="QBH27_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDQ7012506.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            2949..3431
                     /locus_tag="QBH27_000128"
     CDS             2949..3431
                     /locus_tag="QBH27_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDQ7012507.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPDAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLEMDVYVPLA
                     "
     gene            3540..5147
                     /locus_tag="QBH27_000129"
     CDS             3540..5147
                     /locus_tag="QBH27_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ7012508.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            5285..7543
                     /gene="parC"
                     /locus_tag="QBH27_000130"
     CDS             5285..7543
                     /gene="parC"
                     /locus_tag="QBH27_000130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDQ7012509.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYVAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAVIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQGYGDERRSPLHEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            7778..8515
                     /gene="plsC"
                     /locus_tag="QBH27_000131"
                     /gene_synonym="parF"
     CDS             7778..8515
                     /gene="plsC"
                     /locus_tag="QBH27_000131"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDQ7012510.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            8590..10002
                     /gene="ftsP"
                     /locus_tag="QBH27_000132"
     CDS             8590..10002
                     /gene="ftsP"
                     /locus_tag="QBH27_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDQ7012511.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(10073..11377)
                     /locus_tag="QBH27_000133"
     CDS             complement(10073..11377)
                     /locus_tag="QBH27_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDQ7012512.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(11388..11870)
                     /locus_tag="QBH27_000134"
     CDS             complement(11388..11870)
                     /locus_tag="QBH27_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDQ7012513.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNFH
                     "
     gene            complement(11912..12895)
                     /locus_tag="QBH27_000135"
     CDS             complement(11912..12895)
                     /locus_tag="QBH27_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDQ7012514.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILKSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYSDM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            complement(12968..13978)
                     /locus_tag="QBH27_000136"
     CDS             complement(12968..13978)
                     /locus_tag="QBH27_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HDQ7012515.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDADTAQQVTDVLIHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LALEEGLGFVSVKNTSHCGALSYFAEMVTNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKDGYGTTDPHKIE
                     NLLPFGQHKGSGIALAIDALTGMLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAQMVTELHAVKPAPGIEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            complement(13989..15005)
                     /locus_tag="QBH27_000137"
     CDS             complement(13989..15005)
                     /locus_tag="QBH27_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDQ7012516.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREKKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            complement(15002..16474)
                     /locus_tag="QBH27_000138"
     CDS             complement(15002..16474)
                     /locus_tag="QBH27_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ7012517.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDVVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            complement(16797..17546)
                     /locus_tag="QBH27_000139"
     CDS             complement(16797..17546)
                     /locus_tag="QBH27_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDQ7012518.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIISLQENAIAAESDRFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            17798..20017
                     /locus_tag="QBH27_000140"
     CDS             17798..20017
                     /locus_tag="QBH27_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDQ7012519.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKS
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICSHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(20059..20316)
                     /gene="yqhH"
                     /locus_tag="QBH27_000141"
     CDS             complement(20059..20316)
                     /gene="yqhH"
                     /locus_tag="QBH27_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDQ7012520.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(20367..21293)
                     /locus_tag="QBH27_000142"
     CDS             complement(20367..21293)
                     /locus_tag="QBH27_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDQ7012521.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALILNDNLTLPGNIGWLDYDPVCDCQDFGNLVLESVAITQTD
                     ADHADAAVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     TIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            complement(21493..22320)
                     /gene="dkgA"
                     /locus_tag="QBH27_000143"
     CDS             complement(21493..22320)
                     /gene="dkgA"
                     /locus_tag="QBH27_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDQ7012522.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            complement(22425..23588)
                     /gene="yqhD"
                     /locus_tag="QBH27_000144"
     CDS             complement(22425..23588)
                     /gene="yqhD"
                     /locus_tag="QBH27_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDQ7012523.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            23782..24681
                     /gene="yqhC"
                     /locus_tag="QBH27_000145"
     CDS             23782..24681
                     /gene="yqhC"
                     /locus_tag="QBH27_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDQ7012524.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            complement(24721..25380)
                     /locus_tag="QBH27_000146"
     CDS             complement(24721..25380)
                     /locus_tag="QBH27_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ7012525.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(25520..26707)
                     /gene="metC"
                     /locus_tag="QBH27_000147"
     CDS             complement(25520..26707)
                     /gene="metC"
                     /locus_tag="QBH27_000147"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDQ7012526.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            26959..27693
                     /gene="exbB"
                     /locus_tag="QBH27_000148"
     CDS             26959..27693
                     /gene="exbB"
                     /locus_tag="QBH27_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDQ7012527.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            27700..28125
                     /gene="exbD"
                     /locus_tag="QBH27_000149"
     CDS             27700..28125
                     /gene="exbD"
                     /locus_tag="QBH27_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDQ7012528.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28164..29048)
                     /gene="yghA"
                     /locus_tag="QBH27_000150"
     CDS             complement(28164..29048)
                     /gene="yghA"
                     /locus_tag="QBH27_000150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDQ7012529.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            29239..29733
                     /locus_tag="QBH27_000151"
     CDS             29239..29733
                     /locus_tag="QBH27_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDQ7012530.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(29773..30813)
                     /gene="gpr"
                     /locus_tag="QBH27_000152"
     CDS             complement(29773..30813)
                     /gene="gpr"
                     /locus_tag="QBH27_000152"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDQ7012531.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            30970..31857
                     /locus_tag="QBH27_000153"
     CDS             30970..31857
                     /locus_tag="QBH27_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDQ7012532.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKSNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            31976..32263
                     /locus_tag="QBH27_000154"
     CDS             31976..32263
                     /locus_tag="QBH27_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDQ7012533.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            32452..33570
                     /gene="hybO"
                     /locus_tag="QBH27_000155"
     CDS             32452..33570
                     /gene="hybO"
                     /locus_tag="QBH27_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDQ7012534.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            33573..34559
                     /gene="hybA"
                     /locus_tag="QBH27_000156"
     CDS             33573..34559
                     /gene="hybA"
                     /locus_tag="QBH27_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDQ7012535.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINSPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            34549..35727
                     /gene="hybB"
                     /locus_tag="QBH27_000157"
     CDS             34549..35727
                     /gene="hybB"
                     /locus_tag="QBH27_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDQ7012536.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            35724..37427
                     /gene="hybC"
                     /locus_tag="QBH27_000158"
     CDS             35724..37427
                     /gene="hybC"
                     /locus_tag="QBH27_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDQ7012537.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            37427..37921
                     /locus_tag="QBH27_000159"
     CDS             37427..37921
                     /locus_tag="QBH27_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDQ7012538.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            37914..38402
                     /gene="hybE"
                     /locus_tag="QBH27_000160"
     CDS             37914..38402
                     /gene="hybE"
                     /locus_tag="QBH27_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDQ7012539.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVANPDVPHAGRRALLFGRRSGE
                     NA"
     gene            38395..38736
                     /gene="hypA"
                     /locus_tag="QBH27_000161"
     CDS             38395..38736
                     /gene="hypA"
                     /locus_tag="QBH27_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDQ7012540.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            38749..38997
                     /gene="hybG"
                     /locus_tag="QBH27_000162"
     CDS             38749..38997
                     /gene="hybG"
                     /locus_tag="QBH27_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDQ7012541.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(39067..39933)
                     /gene="yghU"
                     /locus_tag="QBH27_000163"
     CDS             complement(39067..39933)
                     /gene="yghU"
                     /locus_tag="QBH27_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDQ7012542.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            40138..41997
                     /gene="gss"
                     /locus_tag="QBH27_000164"
     CDS             40138..41997
                     /gene="gss"
                     /locus_tag="QBH27_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDQ7012543.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            42289..43788
                     /gene="pitB"
                     /locus_tag="QBH27_000165"
     CDS             42289..43788
                     /gene="pitB"
                     /locus_tag="QBH27_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDQ7012544.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(43837..44529)
                     /locus_tag="QBH27_000166"
     CDS             complement(43837..44529)
                     /locus_tag="QBH27_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDQ7012545.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRSVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNIEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLIDAEK
                     AITDFMTARGYH"
     gene            44703..45416
                     /locus_tag="QBH27_000167"
     CDS             44703..45416
                     /locus_tag="QBH27_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDQ7012546.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNMTALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIQAALS"
     gene            45448..46221
                     /locus_tag="QBH27_000168"
     CDS             45448..46221
                     /locus_tag="QBH27_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012547.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTQSMKTKSPA
                     LWAAVIMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATG
                     KISMFLAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIG
                     YNGTKILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            46267..47616
                     /locus_tag="QBH27_000169"
     CDS             46267..47616
                     /locus_tag="QBH27_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDQ7012548.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQIWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            47616..48440
                     /locus_tag="QBH27_000170"
     CDS             47616..48440
                     /locus_tag="QBH27_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012549.1"
                     /translation="MMRKYFPLEASERLFVAVEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            48452..49012
                     /locus_tag="QBH27_000171"
     CDS             48452..49012
                     /locus_tag="QBH27_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDQ7012550.1"
                     /translation="MSSRLIIALIIMLLAQGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            49043..50113
                     /gene="lptF"
                     /locus_tag="QBH27_000172"
     CDS             49043..50113
                     /gene="lptF"
                     /locus_tag="QBH27_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDQ7012551.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGNPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            50110..51189
                     /gene="lptG"
                     /locus_tag="QBH27_000173"
     CDS             50110..51189
                     /gene="lptG"
                     /locus_tag="QBH27_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDQ7012552.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(51224..52396)
                     /locus_tag="QBH27_000174"
     CDS             complement(51224..52396)
                     /locus_tag="QBH27_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDQ7012553.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(52396..52644)
                     /locus_tag="QBH27_000175"
     CDS             complement(52396..52644)
                     /locus_tag="QBH27_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDQ7012554.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(52676..53590)
                     /locus_tag="QBH27_000176"
     CDS             complement(52676..53590)
                     /locus_tag="QBH27_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDQ7012555.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADAITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(53587..55317)
                     /locus_tag="QBH27_000177"
     CDS             complement(53587..55317)
                     /locus_tag="QBH27_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDQ7012556.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            55679..56821
                     /locus_tag="QBH27_000178"
     CDS             55679..56821
                     /locus_tag="QBH27_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ7012557.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(56828..57592)
                     /gene="glcC"
                     /locus_tag="QBH27_000179"
     CDS             complement(56828..57592)
                     /gene="glcC"
                     /locus_tag="QBH27_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDQ7012558.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLEFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            57843..59342
                     /gene="glcD"
                     /locus_tag="QBH27_000180"
     CDS             57843..59342
                     /gene="glcD"
                     /locus_tag="QBH27_000180"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDQ7012559.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTLKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            59342..60394
                     /gene="glcE"
                     /locus_tag="QBH27_000181"
     CDS             59342..60394
                     /gene="glcE"
                     /locus_tag="QBH27_000181"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDQ7012560.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            60405..61628
                     /gene="glcF"
                     /locus_tag="QBH27_000182"
     CDS             60405..61628
                     /gene="glcF"
                     /locus_tag="QBH27_000182"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDQ7012561.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            61633..62037
                     /locus_tag="QBH27_000183"
     CDS             61633..62037
                     /locus_tag="QBH27_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDQ7012562.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            62059..64230
                     /gene="glcB"
                     /locus_tag="QBH27_000184"
     CDS             62059..64230
                     /gene="glcB"
                     /locus_tag="QBH27_000184"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDQ7012563.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHSILTKEQVQASLENMAKVVDQQNAGDPAYRPMSG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            64585..66267
                     /gene="glcA"
                     /locus_tag="QBH27_000185"
     CDS             64585..66267
                     /gene="glcA"
                     /locus_tag="QBH27_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDQ7012564.1"
                     /translation="MVTRTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            66752..71308
                     /gene="sslE"
                     /locus_tag="QBH27_000186"
     CDS             66752..71308
                     /gene="sslE"
                     /locus_tag="QBH27_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDQ7012565.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRVTG
                     ATCNGESSDGFTFTPGEDVTCVAGNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELA
                     ASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGR
                     LVDSMGNGVVGVNYYTSSGRGVTGENGKFNFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSQAGKNDEREVPDVVRKVFAEYPNVINEIINLSLSNGEA
                     LSEGDQTFERTNEFLEQFESGQAKEIDTAICDSLGGCNSQRWFSLTARNVNEGQIQGV
                     INKLWGVDKDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPH
                     YNSILRCPNGYSWEGGVDKNGQCTRNSDSNDMKHFMQNVLRYLSDDKWTPDAKASMTV
                     GTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYV
                     TQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTG
                     EVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKIFE
                     KFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADL
                     GTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNVSVWLWNKIEYRYENDKDDELGF
                     KTFTEFLNCYANDAYTGGTQCSDELKKSLVDNNMIYGEKSVNKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIESSASVPVTVTVALADDLTGREKHEVALNRPPKVTKTYDLKANDKVTFK
                     VPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDAFVYT
                     TPKKNLEASNFTGGVAEFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKQWYPEGDLPEFYSDREGMKGWNLFQLMHRKARGDDVGKTKFGERNYCAES
                     NGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMNFEGGVSQSAYETLAA
                     LNLPKPQQGPETINQVTEHKMSAE"
     gene            71438..72247
                     /gene="pppA"
                     /locus_tag="QBH27_000187"
     CDS             71438..72247
                     /gene="pppA"
                     /locus_tag="QBH27_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDQ7012566.1"
                     /translation="MLFDVFQQYSAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKINLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            72313..72723
                     /gene="gspS2"
                     /locus_tag="QBH27_000188"
     CDS             72313..72723
                     /gene="gspS2"
                     /locus_tag="QBH27_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDQ7012567.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            72741..73700
                     /gene="gspC"
                     /locus_tag="QBH27_000189"
     CDS             72741..73700
                     /gene="gspC"
                     /locus_tag="QBH27_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDQ7012568.1"
                     /translation="MARVVFRDARIYLIQLLTKIRHTLSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEKRPTKAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            73730..75790
                     /gene="gspD"
                     /locus_tag="QBH27_000190"
     CDS             73730..75790
                     /gene="gspD"
                     /locus_tag="QBH27_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDQ7012569.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLALVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            75790..77283
                     /gene="gspE"
                     /locus_tag="QBH27_000191"
     CDS             75790..77283
                     /gene="gspE"
                     /locus_tag="QBH27_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDQ7012570.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            77283..78506
                     /gene="gspF"
                     /locus_tag="QBH27_000192"
     CDS             77283..78506
                     /gene="gspF"
                     /locus_tag="QBH27_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDQ7012571.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQHLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            78523..78978
                     /gene="gspG"
                     /locus_tag="QBH27_000193"
     CDS             78523..78978
                     /gene="gspG"
                     /locus_tag="QBH27_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDQ7012572.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            78982..79545
                     /gene="gspH"
                     /locus_tag="QBH27_000194"
     CDS             78982..79545
                     /gene="gspH"
                     /locus_tag="QBH27_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDQ7012573.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            79542..79913
                     /gene="gspI"
                     /locus_tag="QBH27_000195"
     CDS             79542..79913
                     /gene="gspI"
                     /locus_tag="QBH27_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDQ7012574.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            79910..80515
                     /gene="gspJ"
                     /locus_tag="QBH27_000196"
     CDS             79910..80515
                     /gene="gspJ"
                     /locus_tag="QBH27_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDQ7012575.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMALQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            80512..81489
                     /gene="gspK"
                     /locus_tag="QBH27_000197"
     CDS             80512..81489
                     /gene="gspK"
                     /locus_tag="QBH27_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDQ7012576.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHQAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            81486..82664
                     /gene="gspL"
                     /locus_tag="QBH27_000198"
     CDS             81486..82664
                     /gene="gspL"
                     /locus_tag="QBH27_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDQ7012577.1"
                     /translation="MSSILEIFFPLCDSDTVRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPFSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            82666..83202
                     /gene="yghD"
                     /locus_tag="QBH27_000199"
     CDS             82666..83202
                     /gene="yghD"
                     /locus_tag="QBH27_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDQ7012578.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAA
                     VFLFCAVYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIIAARKSGTTTQAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVLRLEFGRG"
     gene            84260..85036
                     /locus_tag="QBH27_000200"
     CDS             84260..85036
                     /locus_tag="QBH27_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7012579.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYDAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGIGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            85033..85707
                     /locus_tag="QBH27_000201"
     CDS             85033..85707
                     /locus_tag="QBH27_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7012580.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVAIVFKNSYIIGYYENVQSGIDEYKMY
                     QALDIE"
     gene            86085..86241
                     /locus_tag="QBH27_000202"
                     /pseudo
     CDS             86085..86241
                     /locus_tag="QBH27_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            86692..87408
                     /locus_tag="QBH27_000203"
     CDS             86692..87408
                     /locus_tag="QBH27_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012581.1"
                     /translation="MIVANMSSYPPRKKELVHSIQSLHAQVDKINLCLNEFEEIPEEL
                     DGFSKLNPVIPDKDYKDVGKFIFPCAKNDMIVLTDDDIIYPPDYVEKMLNFYNSFAIF
                     NCIVGIHGCIYIDAFDGDQSKRKVFSFTQGLLRPRVVNQLGTGTVFLKADQLPSLKYM
                     DGSQRFVDVRFSRYMLENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKE
                     TQAIAGYSKLNLELVYNVEG"
     gene            87843..>88951
                     /locus_tag="QBH27_000204"
     CDS             87843..>88951
                     /locus_tag="QBH27_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HDQ7012582.1"
                     /translation="MNKLVLVGHPGSKYQIVEHFLKEIGMNSPNYSTSNKISPEYITA
                     SLCQFYQTPEVNDVVDEREFSAVQVSTMWDSMVLELMMNNLNNKLWGWADPSIIFFLD
                     FWKNIDKSIKFIMIYDHPKYNLMRSVNNAPLSLNINNSVDNWIAYNKRLLDFFLENKE
                     RCVLINFEAFQSNKKNIIKPLSNIIKIDNLMSAHYKNSILFDVVENNDYTKSNEIALL
                     EKYTTLFSLSANETEITFNDTKVSEYLVSELIKERTEVLKLYNELQAYANLPYIETSK
                     DNVSAEAALWEVVEERNSIFNIVSHLVQESKKKDADIELTKSIFKKRQFLLLNRINEL
                     KKEKEEVIKLSKINHNDVVRQEKYPDDIEKKINDI"
BASE COUNT        22003 a        21498 c        23319 g        22131 t
ORIGIN      
        1 agcactatta tatataatgc ttacttattg gttaatagat tctttttttg ctttcaataa
       61 gtatgactgg atgcttgagt cgggagacaa tatttgcagt ataccttctg tttcaggcga
      121 agatcgtata ttacaagcaa tgattgcagc attttttctt ctgacaccac ttattattct
      181 tatcttaaga aaactcttta tgcgggaaat gttcgagttt tggctatatg tattctcgct
      241 cgttatttgt ctgggttgtg gttggtggct tttttgggga cggtttatat tttgctatta
      301 gtaaagtcta tgagagtcgt tttaacggct ctcatagaca gagaagttac cagcttacct
      361 tcgcttcaaa tccaccttgt ttcgcattcc caaaatcaac attcatgcca tgcaatttgg
      421 cgattcgctg gacaatcgat agcccaagtc cactgccggt agcggtttgt ccgggtgggc
      481 gatagaagcg ttcgccaatt cgcgccagtg cctctggtgt cacaccaggg ccgttatccc
      541 tcacgatgaa attatcggca ttcagcgtga cgtctaccac gctaccttgt ggactgtaac
      601 gcacggcgtt atccagcaaa tttcgcacca acaaacttag caatagcggt tgtccggtgc
      661 gtttgatgcc gttggcattg agtgtcagtc gcacgtcaat gttcgcctgc tgcgccgtgt
      721 ggtaaatatc catcaccgac gattgcagga gatcttcaag cgggatctcc gcgacgtcct
      781 gaaggttatc cagtgagtcc agccgcgata gcgtgagcag ttgatcaacc agacgagtag
      841 cgcgatcgat cccggaatgt aattggagca gcgctttttt ccgcgcctgc ggatcatcgt
      901 cagagagctg agcaacttcg gtttgcactt tcagcgccgt taacgggcta cgaagttcgt
      961 gagctgcgtc ggaggtaaag cgtcgttcac gaaccatcat cgcatgtgtg cgggcgaaca
     1021 gttgatttag cgactcaacc agtgggcgca cttcgctggg tacgcccgtc gcgtttagtg
     1081 gtttttccga gtctgggtca cgcatacgta gtgccagcgc cagtttgttc agcggcgcga
     1141 gttcacgacc cagtagcacc atcatgatga ttaacataat cggcagtgcg accagccacg
     1201 ggatcaattg tccggcaaca atcgccagcg ccatgtcttc acggtattcc cattcctgac
     1261 ccacaacgat gcgatatttg ccatcaggtg aggtcatcca gacaaaacgc cagggatcgt
     1321 cttcaccgac cagttgcccg tcagcaaaac cttcccgctg atagctatag ggaatatctt
     1381 ctccgttatc gccatcgtta aggaccattc tgccgtcgtg ggtaaagatg gcaaaagtca
     1441 gcgcatcgtc atcaacatga ccgtgtttta atttatttgg cgtctgtgcc atgcgatccg
     1501 ccgcgttgat ttcgttgaga tcgagcgtac ttaaccgttt ggcaaacagc atcagttggg
     1561 tgtcgaacaa ctcatcgacg ttatccgttg tttgtttcca ggcgacaaag ctggaaagca
     1621 gccaggtcac cgaggccaga attaaaaaga ttagcgtcag cctgactcgc agactaagac
     1681 gttgggtaaa tttcatttct cacctaatgt gtagccaata ccatgcacgg tacgaataaa
     1741 atcactaccg agtttgcgtc gcagatgatg cacatgcact tcaacggcat tactggtgac
     1801 ctcttcgtcc caggtataca gtttctcttc aatcagtttg cgcggcagta cccgaccagc
     1861 gttacgcatc agtaattcca gcagggcaaa ttcttttggt ttcagcgtta agggttcgcc
     1921 agccagcgtg gcgatacgtt tgccggggtc gagcatgacg ttgccgtggc gcagctcgtt
     1981 gctggcctgg ccgttggttc ggcgcatcag agcttccagc ctggcggcga cttctatcaa
     2041 cgcaaaaggt ttacacagat aatcgtcagc tcccagacgc agcccttcta cacgttccgc
     2101 cagcgcatcg cgcgcagtca ggatcaatac aggctcacgc tgaccttttt ctcgccattc
     2161 gcgcaaaata tcgcgaccat ccatgcctgg taaggttaaa tccaggatca ccgcatcata
     2221 aggtgcgcta tatagcgcct cttttccctg acgaccttgt gtaaaccagt cgacgctaaa
     2281 acccatttta ctaaggcccg ttttgatgcc gtcgccaatc agcatgtcat cttctatcag
     2341 taaaattcgc attttttcat ccctgcgata accgtatttg ctgccagtaa accgtgttgt
     2401 aacgcgctct gtacagcatt aaattttatt tttttccctt aagaagttgt taaggactat
     2461 cttgttaaat gctcggaaac agacattaaa gggagtaata aacatgaaaa aattcgcagc
     2521 agtaatcgca gtaatggccc tgtgcagtgc accggttatg gcagcagagc agggaggttt
     2581 ttctggccca tcggcaacgc aaagtcaggc cggaggattc caggggccga acggcagcgt
     2641 aacgactgta gaaagcgcaa aatccctgcg tgacgacacc tgggtaaccc tacgcggcaa
     2701 tatcgttgaa cgcatctctg acgatctcta cgtgttcaaa gatgccagcg gtactatcaa
     2761 tgttgatatc gaccataaac gctggaacgg cgtgacggtg acgccgaaag atacggttga
     2821 gattcagggt gaagtcgata aagactggaa ctctgttgaa attgacgtca aacagatccg
     2881 caaagtaaat ccgtaattgt taccgctccc gggacgcgtt cccgggaata atttcgcagg
     2941 gaggcaaaat gacaaacctg acactggatg taaatattat cgatttccca tcaatccctg
     3001 tggcgatgtt gccgcaccgc tgtagccctg agttgctcaa ctacagcgtg gcgaaattta
     3061 tcatgtggcg taaagagacg gggctttctc ctgttaacca aagccagact tttggcgtcg
     3121 cctgggacga ccctgccacc accgcaccag atgcgtttcg ctttgatatc tgcggcagcg
     3181 ttagcgaacc gatccccgat aatcgttatg gcgtaagtaa tggtgaactt accggtggac
     3241 gttatgctgt ggcccgccat gttggcgagc tggacgatat ttcacacacg atatggggca
     3301 tcattcgcca ctggctgcct gcaagcggcg agaaaatgcg taaagcaccg attctgtttc
     3361 actacaccaa tcttgccgaa gggatgacag agcagcgact ggaaatggat gtttatgtac
     3421 cgttagcgtg atggggaatc tttaccggag tgtgagccaa atcactcgcg ataagtttga
     3481 gcaaacaatg ccacaggcgt attgttgtcg ggcttataat ttctccggta aggaatacca
     3541 tgtatacgcg aaatttatta tggctggtca gcctggtaag tgcggctcct ctctacgctg
     3601 ctgacgttcc cgccaacaca ccgctcgccc cgcaacaagt ctttcgttac aacaatcata
     3661 gcgacccagg cacgctcgac ccgcaaaagg tagaggagaa taccgccgcg cagattgtgc
     3721 tggatctgtt tgaaggtctg gtatggatgg acggtgaagg ccaggtgcag cccgctcagg
     3781 ctgaacgctg ggagatactt gacggcggca agcgctatat tttccatctg cgtagcggtt
     3841 tgcagtggtc agacggtcag cctctgacgg cagaggattt tgtcctcggc tggcagcgcg
     3901 cggttgaccc gaaaacggca agcccttttg ctggctatct ggcacaggcg cacattaaca
     3961 atgccgcggc tattgttgcg ggtaaagcag atgttacatc gctgggtgtc aaagcgatgg
     4021 atgatcgtac tcttgaagtt acgcttgagc agccggttcc ttggttcacg acgatgctcg
     4081 cctggccgac gctgttcccg gttcctcatc atgtcatcgc taaacatggc gatagctgga
     4141 gtaagccaga gaacatggtt tacaacggtg cctttgtgct tgataaatgg gtagttaacg
     4201 aaaagattac tgcacgcaaa aatccaaagt accgcgatgc gcaacataca gtattgcaac
     4261 aggttgagta tctggcgctg gataattcgg tcaccggcta taaccgctat cgcgcgggag
     4321 aggtcgatct cacctgggtt ccggcgcagc aaattcccgc cattgaaaaa tcactgcctg
     4381 gcgagctacg aattatcccg cgtctgaaca gcgaatatta caactttaac cttgagaaac
     4441 cgccatttaa cgatgtgcgg gtgcgtcggg cgctatatct tacggttgat cgacagctta
     4501 ttgcgcaaaa ggtactgggg ttgagaacgc ccgcaaccac gctgacgccg ccagaggtaa
     4561 aaggctttag cgcgacgacg ttcgatgaac tgcaaaagcc aatgagtgag cgcgtcgcga
     4621 tggcaaaagt cttgctgaaa caggcgggat acgacgcctc tcatccgcta cgctttgagc
     4681 tgttctacaa caagtacgat ctgcatgaaa agaccgcgat agcgttgtct tccgaatgga
     4741 aaaaatggct gggtgcacag gtgacgctgc gcacaatgga gtggaaaacc tatcttgatg
     4801 cccgacgagc cggtgatttt atgctgtctc ggcagtcgtg ggatgcgacg tacaatgatg
     4861 cctccagctt cctgaacacg ctcaaaagcg atagtgaaga aaacgtcggc cactggaaaa
     4921 acgcgcagta tgacgcctta ctaaaccagg cggcacaaac ggctgatgca acaaagcgta
     4981 atgcgctgta tcagcaggca gaagtgatca tcaaccagca ggcaccgctg attcccatct
     5041 actatcagcc gttaatcaaa ctgcttaaac cctacgttgg cggttttccg ctgcataatc
     5101 cccaggatta tgtctacagc aaagagttgt atatcaaggc acattgatgc ctttatcgcc
     5161 gccggagcac gtccggcggc gctattcacg ctgatctcct gtgactcgac gcggcagata
     5221 atgtagtatc tccggcaata ttgccccttt gaaggctggc gaataagttg aggaatcaga
     5281 attaatgagc gatatggcag agcgccttgc gctacatgaa tttacggaaa acgcctactt
     5341 aaactactcc atgtacgtga tcatggaccg tgcgttgccg tttattggtg atggtctgaa
     5401 acctgttcag cgccgcattg tgtatgcgat gtctgagctg ggcctgaatg ccagcgccaa
     5461 atttaaaaaa tcggcccgta ccgtcggtga cgtactgggt aaataccatc cgcacggcga
     5521 tattgcctgt tatgtagcga tggtcctgat ggcgcagccg ttctcttacc gttatccgct
     5581 ggttgatggt caggggaact ggggggcgcc ggacgatccg aaatcgttcg cggcaatgcg
     5641 ttacaccgaa tcccggttgt cgaaatattc cgagctgcta ttgagcgagc tggggcaggg
     5701 gacggctgac tgggtgccaa acttcgacgg cactttgcag gagccgaaaa tgctgcctgc
     5761 ccgtctgcca aacattttgc ttaacggcac caccggtatt gccgtcggca tggcgaccga
     5821 tattccaccg cataacctgc gtgaagtggc tcaggcggta attgccttaa tcgaccagcc
     5881 gaaaaccacg ctcgatcagc tgctggatat cgtgcagggg ccggattatc cgactgaagc
     5941 ggaaattatc acttcgcgcg ccgagattcg taaaatctac gagaacggac gtggttcagt
     6001 gcgtatgcgc gcggtttgga agaaggaaga tggcgcggtg gttatcagcg cattgccgca
     6061 tcaggtttca ggtgcgcgcg tactggagca aattgctgcg caaatgcgca acaaaaagct
     6121 gccgatggtt gacgatctgc gcgatgaatc tgaccacgag aacccgaccc gtctggtgat
     6181 tgtgccgcgt tccaaccgcg tggatatgga tcaggtgatg aaccacctct ttgctaccac
     6241 cgatctggaa aagagctatc gcattaacct caatatgatc ggtctggatg gtcgtccggc
     6301 ggtgaaaaac ctgctggaaa tcctctccga atggctggtg ttccgccgcg ataccgtgcg
     6361 ccgccgactg aactatcgtc tggagaaagt tctcaagcgc ctgcatatcc tcgaaggttt
     6421 gctggtggcg tttctcaata tcgacgaagt gattgagatc attcgtaatg aagatgaacc
     6481 gaaaccggcg ctgatgtcgc ggtttggcct tacggaaacc caggcggaag cgatcctcga
     6541 actgaaactg cgtcatcttg ccaaactgga agagatgaag attcgcggtg agcagagtga
     6601 gctggaaaaa gagcgcgacc agttgcaggg cattttggct tccgagcgta aaatgaataa
     6661 cctgctgaag aaagaactgc aggcagacgc gcaaggctac ggtgacgaac gtcgttcgcc
     6721 gttgcatgaa cgcgaagaag cgaaagcgat gagcgagcac gacatgctgc cgtctgaacc
     6781 tgtcaccatt gtgctgtcgc agatgggctg ggtacgcagc gctaaaggcc atgatatcga
     6841 cgcgccgggc ctgaattata aagcgggtga tagcttcaaa gcggcggtga aaggtaagag
     6901 caaccaaccg gtagtgtttg ttgattccac cggtcgtagc tatgccatcg acccgattac
     6961 gctgccgtcg gcgcgtggtc agggcgagcc gctcaccggc aaattaacgt tgccgcctgg
     7021 agcgaccgtt gaccatatgc tgatggaaag cgacgatcag aaactgctga tggcttccga
     7081 tgcgggttac ggtttcgtct gcacctttaa cgatctggtg gcgcgtaacc gtgcaggtaa
     7141 ggctttgatc accttaccgg aaaatgccca tgttatgccg ccggtggtga ttgaagatgc
     7201 ttccgatatg ctgctggcaa tcactcaggc aggccgtatg ttgatgttcc cggtaagcga
     7261 tctgccgcag ctgtcgaagg gcaaaggcaa caagattatc aacattccat cggcagaagc
     7321 cgcgcgtggc gaggatggcc tggcgcaact gtacgttctg ccgccgcaaa gcacgctgac
     7381 cattcatgtt gggaaacgca aaattaaact gcgtccggaa gagctacaga aagtcactgg
     7441 cgaacgtgga cgccgcggta cgttgatgcg cggtttgcag cgtatcgatc gtgttgagat
     7501 cgactctcct cgccgtgcca gcagcggtga tagcgaagag taatatgccg gggagggcgc
     7561 tgtttcctcc cgcttgcaag gaacgccgga tgaaacgtta agtcgtcatc cggcagcgta
     7621 ttcaatcccc ggaaacgata aaaattccct tgaaaccgtt gtttattcat gcgttgcgat
     7681 taacaatacg cttttccaga gagcggcttt taacaatgcc ctaacctgat tttcaggtga
     7741 cgtacaatgc cagtctctca gaccttcaga gggtgttatg ctatatatct ttcgtcttat
     7801 tattaccgtg atttacagca tcttagtctg tgtattcggc tccatttact gccttttcag
     7861 cccgcgtaat ccgaaacatg tggccacctt tgggcatatg tttggtcgtc ttgcgccgct
     7921 tttcggcctg aaagtggagt gccgcaaacc tgcagacgct gaaaactacg gcaatgctat
     7981 ctatattgct aaccaccaga acaattatga catggtgacg gcatcgaaca ttgtgcagcc
     8041 gccgacagtg acggtaggta aaaagagttt gctgtggatc cccttcttcg ggcagttgta
     8101 ttggttaacc ggcaacttat tgatcgacag aaacaatcgc actaaagctc acggcactat
     8161 tgcggaagta gtgaatcact tcaaaaaacg ccgtatttcc atctggatgt tcccggaagg
     8221 aacccgcagc cgtggtcgcg gcctgctgcc gttcaagacc ggggcatttc acgcggcaat
     8281 tgcggcgggc gtcccgatta tacccgtgtg cgtctctaca acttcgaata agattaatct
     8341 taatcggcta cacaacggtc tggtgattgt cgaaatgctg ccgccaattg acgtcagtca
     8401 gtatggcaaa gatcaggttc gcgagttggc tgcccattgt cgttcgataa tggaacaaaa
     8461 aatcgccgag ctcgataaag aagtcgcaga acgcgaagcc gccggaaaag tttaagtcgg
     8521 caatctgtat ttttgcgggg aacactttcc tgcacggtat tactttagcc agttttacat
     8581 ggagcaaata tgtcactcag tcggcgtcag ttcattcagg catcggggat tgcactttgt
     8641 gcaggcgctg ttcccctgaa ggccagcgca gccgggcaac agcaaccgct acccgttccg
     8701 ccgctgcttg aatctcgccg tgggcaaccg ctgtttatga ctgtacaacg tgcgcactgg
     8761 tcatttacgc cagggacacg cgcgtcggtt tggggaatca atggtcgtta cctggggccg
     8821 actatccgcg tctggaaggg cgacgatgtt aagcttattt acagcaaccg cctgacagaa
     8881 aatgtctcaa tgacggtggc cgggctacag gtacctggcc cgctgatggg cggtccggca
     8941 cggatgatgt cgccaaacgc tgactgggca cccgtactgc ccattcgcca gaacgcagct
     9001 actctgtggt atcacgccaa tactcccaac cgcacggctc agcaggtcta taacggcctt
     9061 gccggaatgt ggctggtgga agatgaagtc agtaaatcgc tgcctatccc caaccattat
     9121 ggtgtggatg attttcctgt cattattcag gataaacggc tggataactt tggtacgcca
     9181 gaatataacg aacctggaag cggcggcttt gttggcgata cgctactggt taacggtgta
     9241 caaagcccgt acgttgaagt ctcgcgtggc tgggtgcgct tgcgtctgtt gaacgcgtcg
     9301 aactctcgtc gctatcaact acagatgagc gatggtcgcc cgttacatgt gatttctggc
     9361 gatcagggat tcctgcccgc tcctgtatcg gtgaagcaac tttcgctggc accgggcgag
     9421 cgtcgtgaga ttctggtgga tatgagcaac ggtgatgaag tgtcgattac ctgtggcgaa
     9481 gcggcgagca ttgttgatcg tattcgtggc ttctttgagc catccagcat tctggtttct
     9541 acgctggtgc taacgctgcg cccaaccggc cttctgccgc tggtcacaga cagtcttccg
     9601 atgcgcttgc tgccaactga aatcatggcc ggttcgccga ttcgaagtcg tgatatcagt
     9661 ctgggtgatg acccggggat taatgggcag ttgtgggacg tcaaccgtat tgatgttact
     9721 gcgcagcaag gaacgtggga acgctggacg gtgcgcgcgg acgagccgca agcgttccat
     9781 attgaaggcg tgatgttcca gatccgtaac gtgaatggcg cgatgccgtt cccggaagac
     9841 agaggctgga aagataccgt ttgggttgac ggacaagtgg agctgcttgt ttatttcggt
     9901 cagccttcct gggcgcactt cccgttctac ttcaacagtc agacgctgga aatggcggac
     9961 cgtggctcga ttgggcaact gttggtcaat ccggtaccgt aatggaccaa ttcaatgccc
    10021 cacagagtgg tggggcattg aaaattactc agtatgcagt cttcattgtg tatcaggtcg
    10081 aaaataatcc tggcagcatc agactaagct gcgggaaata acaaataatt gccattacta
    10141 caaacaatgc cagaaacatc ggcagtaatg gttttattgc cctgtcgata ctgaccttac
    10201 caatgctgca tcccacaaac agaatggtgc cgacaggtgg tgtgcaaagg ccaatggtta
    10261 agttgaaaac cataatgatg ccgaagtgaa tcggatcaat ccccagatgc tgggcgatcg
    10321 gcaggaaaat aggtgtaaat atcaggatcg ccggcgtgat atccataaag gtaccaacga
    10381 tcaacagaat gatattgata ataaacagaa tgacccattt gttatccgaa acgcgggtaa
    10441 tcagttcgtt gatcaactca ggaacatcag cattcgtcat ggcccatgac atccccatcg
    10501 agcagcctac cagtaacaga acaattgaac tggttactac cgaatctaac agaatatcat
    10561 tgagcttctt aagagtgatt tcccggtaaa tcatcgccag ggctaaacta taaattaccg
    10621 caattgccga tgcttctgta ggcgtaaata cccccgcgat aataccgcca ataatgataa
    10681 aaataagcat caggctagga attgattcgc ggaatacctg taaaaactga cggtaattaa
    10741 tgcgctcggc caccggataa tggtgacgtc gggcataaag tgcagctaca aacattaagg
    10801 caacagcggt gaggatgccc ggcagatatc cagctaagaa caacgcagcg acagaagtcc
    10861 cgccactggc aagggaataa acaatcaaca cattgctcgg tgggatcatc aggccaatgg
    10921 gggcagaggc aatattaacc gctgctgaaa acgccggatc atatccctct ttttcttgca
    10981 gtggtgacat aatcccacct accgcggcgg ctgacgcaac ggctgaacct gaaatcgcac
    11041 caaacagtgt attcgcgagg atattacaat gagccagtga gccaggaaga cgcccaacta
    11101 atgcttgcgc caggttaacc agtcgtctgg ctataccacc ggtattcata atgtttccgg
    11161 ctaacacgaa gaagggaatg gctaatagcg taaagccatc cagccccaca gtcatctttt
    11221 gcgaaatcac agaaatggcg gcatcgggtg gtaaggcagt aataatagat aacagcgaag
    11281 aaagaccaat cgcaaaagta attggcacac ctattgccag catgacggca aaactgccaa
    11341 ataaaattaa gacggggtag atatattcaa agtccatatt tccctcgtta atgaaaattc
    11401 cgtttacgat agttgtcaat aatattcatt actgaatacc agaccagaat aagcccactg
    11461 acaggcataa cccaatatac tttatccatt ggccatttta atactggaga gatttgtcca
    11521 acatgttgtg ctttttcaac aacaatattc ccgccataga tcatgaaaat aaccgcaaag
    11581 accgtaataa taatttggat aaaactgtca agcgtcagaa cagatgcagc cggaagtttt
    11641 tgagccaaca aatcaatggc cagatgtttt tttaaccccg cagtatatac accgccaagt
    11701 aaaatcatcc acataaataa aatctgggtg aactcatcaa gcacagtgct gggagcgtta
    11761 aaaataaaac gtgcaataac ttgccaggtt acgcagaaca ccataatggc gagaataatg
    11821 cagcaacatc cggcaaggat cttattaaga atatgagtga gtgttcgcat aagtcttcca
    11881 tgaaaaggca ggaattacct gcccattagg gttacatact ttctactagc tcaaggtttt
    11941 tggctgattg tgcatctttc gccttgaatt catcgtataa cggtttgacg cgttctttga
    12001 aagcggattt atcaacatca acaactgttg cgttcatccc tttcattttg tcgtaggctt
    12061 ctttttcagc cgcaggccag atgacgtcct tcatctgcat cattgactca tcggctgctt
    12121 ttaataatgc cgtgcgctct ttatcaccaa gtttattcaa cacgtcggtg ctgataatta
    12181 acacatcagg taccatggtg tgttcatcac gtgaatagac ttttgcgact tcggcgtgac
    12241 gcatatcgga atagacaacg gtattgttct ctccgccatc aaccactcct tgctgaagtg
    12301 cggtgtagag ttcgccttgc gccatcgggg tagcgacacc accgagtgct ttcatcattg
    12361 caatggcgct ggggctttgt tgaaccctga ttttcattcc cgctaaatct tcgggttttg
    12421 taatcggttt attggaataa aaacttctcg caccgccgtc atagtacgta ataccaacaa
    12481 aacctttgct ttctgatgac ttaagtattt ttttacctaa atcactttgc agaacacgat
    12541 aatagtgatc acggtcgcgg aataagaacg gcagactgaa aaggctgtaa tccggggcaa
    12601 atgattctag tgatgcagca ttaaccttaa ccatctgtaa tgcaccggag ttcattaatt
    12661 ccagtgattc acgctcattg cctaatgtcg catttgggta aatgcgaata actaattcac
    12721 cattagaata ctctttggtt ttatccgcta aatagcttaa tgctttatgg acagcatggc
    12781 ttttatcctg attatgactc agttttaatg ttgtttttgc cagtgtggta ttagatatta
    12841 ataaacaact ggttaacagc ataagcgatg ccgtcagagg acgaagtgct ttcatatcct
    12901 gctcctggtt aatataacag aaacgataaa tacagcgctt ttctatcgtt ataataaaaa
    12961 gtagtgatta attctctgcc agataatgga atattcctgc tggaaccgga ataccttcct
    13021 gttggcattt ttctttgtag tgcatttgcg gatcgttcgg cgctaacact ttttcgatac
    13081 cgggagccgg tttaacggca tgcaactccg tgaccatttg cgccatggtt tttgcaaaaa
    13141 caggattgcc gagctttttc ggatcgatgg caataaccaa acttgccagc ttacgcatct
    13201 tgtcataatc accatacatg cgaacaatat ggttgccaaa attcgcattc atcagcatgc
    13261 cagtcagtgc atcaatagcc agcgcaatgc ctgaaccttt gtgttggccg aaaggtagca
    13321 ggttttcaat cttatgcgga tcagtggtgc cataaccgtc tttatctatc gccagccctt
    13381 caccaatatg ttttcctgtt tcctttgcat gaagtatttt gccaaaagcc gtggcactgg
    13441 ttgccatatc aacaatcatc ggatggctgt tttccaccgg gaaaccaaag gcgatggggt
    13501 tagtacccaa aaagcgctcg gcgccgccat ggggagccac gcaggtatcg gtttgcgtca
    13561 ttacgatagc aaccaaccct ttgttggtga ccatctctgc aaaataagat aacgcgccac
    13621 agtgagacgt atttttaacg ctaacaaaac cgagaccctc ttccagggcc agtttaatag
    13681 cgtgctcagt ggcgctaatt aatgccgaat gccccattcc gtcgtcagag tcgagaatgg
    13741 ccactgacgg tgaaatttgc tcaatactaa actgcggggc tttatttaac cctccggcag
    13801 caaggcgggt acaataatgt tcgacacgca taactccatg tgaatgcaca cctgtaatat
    13861 cggcatgtat taaaacatct gtcacctgtt gtgcagtgtc ggcatccagc ccggcggtat
    13921 gtaatttgtg gtgcaccagt gattttaaat tttcttcact gacataaaca gttgtcatat
    13981 atgttccctt aaaaagtgat cacacccttg accatatttt tattttctac aacgttacgc
    14041 tggtaatctt cgccaacact aaagaaattg aacgcctggt ttttcattaa attttcgtga
    14101 attttattgc tgcgcatcag ttcaatcacc aacgcaaaat cttcccgtgt ggcattgcgg
    14161 ctgctgagta acgttgtctc ttttttatgg aaggtcggat cgtcaataac aagttcacca
    14221 atgtacaaac cgatgaaaac aattttgccg ccatgacgaa taagatttac atcatgactc
    14281 attgaagctt tatttcccgt cgcatccagg actacgcaag ccagttcgcc gccaaatact
    14341 tcactaagcg cggcaataaa accttcctgt gttgggtcga agacattaat tgccagatga
    14401 tccacaacgt gctggcgacg ttgacagtca atatcagcaa caacaacatg cgccccttta
    14461 gccctggcga tggctgcggt agccagaccg attggcccag cgccaatcac cagtacgttt
    14521 tgttcagcct tgatctctgc ccgacgaacg gcatgtgcac caatggcgaa acattcaacc
    14581 aatgctccgg cactgtcgct gacctcgtca gggagaggca caaggttgtc ttcacgtacc
    14641 gcaaggtact cactaaagcc cccatcctga tgcacgccat acagcgaaac acgttcgcag
    14701 cagttcgttt tcttttcccg acaggctgcg cactcgccac atggaatgca tgggatgacg
    14761 gaatagcgct ggcctgattg tgctgtttgg cactggctgc ctcgcgaaac cgcttcggcg
    14821 catatttcat gacctaatac acgtgggtag ctaaaaaaag gctgtctgcc ggcaaaagcg
    14881 tgaatatcag taccacaaat acccacagct ttgattttta acagcacttc attatctgct
    14941 ggtgtgggaa tatccttttc cacatattcc ataacgccag gctgctgaca aattaatgtt
    15001 ttcatttctg ctcatccgat aataattggc tgatggtggc cttgatgcct ttgtttttta
    15061 tatttgcaaa ggcgattttt atttcattta taaatggctg atggttgctt aaatcaccaa
    15121 atatggcgct aaggtgcagt aatgcgagcg cctgttgcca tgcgtcttgt tgttccttta
    15181 ttagcgcctg aaattggtca ttcattggat cagcgacaga ctggcttttc tcaacaacat
    15241 caatgacata atgcaaccaa ccagcaacga gcagtgacag aacagcattg ttttgctttt
    15301 gctgatgtaa tttcagccag ggcgtcagtg cacgctgggg aagcttttgt gaaccgtcca
    15361 tggctatttg cccggtacgg tgcgcaacgt tacggttgct aaagcgttca attaacaggt
    15421 cggcgtattc ctggatgttg atatttaagt ctggattaag tgttcgggct tgttcattaa
    15481 tcatcagttg gtgggtaatg gaacgaaaat tagcgtcttc catgcattgc cagataaact
    15541 catagcctgc caaagagccg ttataagcca gaaatgaatg gctaccattt aacatgcgta
    15601 acttcatctc ttcataaggc tgaacattgc tgacaaacat cgcgccggct ttatcccatt
    15661 ccggtcgtcc gcgcacaaaa ttatcttcga tcacccactg acgaaatgat tcgcagacga
    15721 tcccgcaggg atcggcataa ccggttttcg tttccagtaa ggcgaattgc gattcagtca
    15781 ttgccggaac aatgcgatct accatcgtgc ctggaaatga gacattttct tcgatccact
    15841 gagccagggg ctggtccagt tgtcgggcaa aaccgagaac ggcattgcgc gtcagatgcc
    15901 cattgtctgg caagttgtca caggagagca cactaaaagc ggcgagtcct gccgctttac
    15961 gttgttgcaa agcacagacg atcacgccaa tagctgacag aggcgcatcc gggttttgta
    16021 gatcgtgttg gattaatcca ttggtgagat caagtgaacg tgtttgcgga tcgaggcaat
    16081 aacctttctc ggtgatggtt aatgaaacga tttttacctg aggttcagcc agtttatgga
    16141 ttgctgcggc aatgccatct ctgggggtat gaataccgcc agccaccgaa cgcacaaggc
    16201 gtgaggtaga ttgcgatgca gatgtttcca caaccgaaaa cagcccattt tgcgcattta
    16261 agttttcgat cagttgagca tcaccaaaca ggttgatctc aaagatcccc cagcgggtgt
    16321 ccgaaagttg attagtgaga tccgtgtaga cagcctggtg tccgcgatga aaagcgccaa
    16381 aaccaatatg gacaatatct gcctgtaacc cgtcccgagg ccaggtggtt acctgatcat
    16441 agtgggcggc agcgatactt tcgttccctg acattatgct cttactcctg acgacttgtg
    16501 tgtagaccaa tttacataat tcactagacc aaaaacagag agatgcatca cgcttgcaga
    16561 gaaaggtaaa agtacgaaac agaacaacaa ggcgttattt atatctgtat caatgagtta
    16621 tatgaataaa atgaaaggaa agttttttag aggtcagttt ttaggataat tgccgctaat
    16681 ctaagatatc tgatgatagt cgatttatta gaccaattaa aaagagagcg tgtaccagtg
    16741 gagggcgaaa ggcagagccg gcgttatttt tcccgccagc tctggactaa tgaaggttaa
    16801 cgagcgaaga aaaagtcatc aaaatcgctg atggaagcgt cttgtttgaa gattttgatc
    16861 agttcatttt tgctgttttc cagatgtctc caggacgctt ccctggcgag ctctgaatct
    16921 ttctgttgca gagccaggaa aatagcccgg tgatcttcca gccattgcag acgcaaatgc
    16981 ttttcatgca ggtacttgta gttaagtttc ttccagcggg ggttttctgt acgcactgcc
    17041 cgccataatt cagcggcttg tttgatgagt acccgatttt gcgtcgcttc ggcaataatg
    17101 ctatgaaaac gatgatccag gtcttcaaat ctatcacttt ccgcagcgat agcgttttcc
    17161 tgcaaactaa tgattttctt taactcttgt aactcgttaa agctaatctg gctggctgca
    17221 aaaccagtaa tattgctctc aatcacctga cgcgcctgca acagttcaaa cgggccaatt
    17281 tctgaatagg gcattaatga tttctggttg agcttatcgg tggagtcgac aaaaaaaatc
    17341 cctgaccctt gcttcacatt caatacgcct ttcagttcca gcataatgat ggcttcacga
    17401 atggtagttc ggctggtatt aaaccgctcg ctcagttcac gttctgaagg taagcgttct
    17461 ccggctttga atacgcctga atacagcagc tcttcaatct gtagaccaat atcgtaatag
    17521 cggcgctttg taataacctg ttccatacct tgccttactg tgggtgtcct cggatagccg
    17581 atcgtcgtgc aatatgattt gaacgttgct gatcggcata aatagtagcg gcagttgcca
    17641 cgataccgcc agccttaaat gtgcttgccc aagtcgggca gtgaaaaacc tcaccagggt
    17701 atttcgccac cgttcatcaa catcacttca tttccgcgcg tgatacagcg tataatcccc
    17761 ggcctttgat tattttttta ccatttctgg aagcaaaatg agctctatct ccctgatcca
    17821 accggatcgc gacctgttct cctggccgca gtactgggcc gcctgttttg gaccggcacc
    17881 gtttttgccg atgtcacgtg aagagatgga tcaacttggc tgggatagct gcgacatcat
    17941 tttggttact ggcgacgcgt atgtcgatca cccaagcttc gggatggcga tttgcggtcg
    18001 tatgctggaa gcgcagggct ttcgcgtcgg gatcatcgcc cagccagact ggagcagcaa
    18061 agacgacttt atgcgtctgg gtaaaccgaa tctgtttttc ggtgttactg ctggcaacat
    18121 ggactcgatg atcaaccgct ataccgccga tcgccgttta cgtcatgacg atgcctacac
    18181 gccagataat gtcgcgggta agcgtccgga tcgcgccaca ctggtttata cccaacgttg
    18241 taaagaggcg tggaaagatg tgccggtgat cctcggcggt attgaggcca gcctgcgccg
    18301 taccgcgcac tatgattact ggtcggatac cgtgcgccgt tccgtgctgg tggattcgaa
    18361 agccgacatg ctgatgtttg gtaacggtga gcgtccgctg gtggaagtgg cgcaccgtct
    18421 ggcgatgggc gagccgatta gtgaaatccg cgatgtgcgt aataccgcga ttatcgtaaa
    18481 agaggcgttg ccaggctgga gcggtgtgga ttccacccgt cttgataccc ctgggaaaat
    18541 cgacccaatc ccgcatccgt atggcgaaga tttgccgtgc gcggataaca aaccggtagc
    18601 tccgaaaaag caggaagcca aatctgtaac cgtgcagcca ccacgcccga aaccgtggga
    18661 aaaaacctac gtgttgctgc cttctttcga gaaagtgaag ggcgataaag tgctgtacgc
    18721 ccatgcttcg cgcattctgc accacgaaac taacccaggc tgcgcccgcg cattgatgca
    18781 aaaacatggc gaccgctatg tatggatcaa cccgcctgct attccgcttt ctaccgaaga
    18841 gatggatagc gtctttgcgc tgccgtacaa gcgcgtgccg catccggctt acggtaatgc
    18901 ccgaattccg gcttacgaaa tgattcgttt ctcggtcaac attatgcgtg gctgctttgg
    18961 cggctgctct ttctgttcta ttaccgagca cgaagggcgc attattcaga gccgttccga
    19021 agattcgatt attaatgaga tcgaagcgat ccgcgacact gttccaggtt ttacgggcgt
    19081 gatttccgat ctcggtgggc ctactgccaa catgtatatg ttgcgctgca aatcgccacg
    19141 cgctgagcaa acctgccgtc gtttgtcgtg cgtttatccg gatatttgtt cgcacatgga
    19201 cactaatcat gaaccgacca tcaatctcta tcgccgtgcg cgtgatctga aaggcattaa
    19261 aaagatcctg attgcctctg gtgtgcgtta tgacatcgct gtagaagatc cgcgctatat
    19321 caaagagctg gcgacccatc acgttggcgg ttatctgaag attgccccgg aacataccga
    19381 agaagggccg ttatcgaaga tgatgaagcc gggcatgggc agctatgacc gctttaaaga
    19441 gctgttcgat acttactcga aacaggcagg taaagaacag tatctgatcc cgtatttcat
    19501 ctccgcgcac ccgggtacgc gtgatgaaga tatggtgaat ctggcgctat ggctgaaaaa
    19561 gcatcgcttc cgcctcgacc aggtgcagaa cttctatccg tcgccgctgg ctaactcgac
    19621 caccatgtat tacaccggca aaaacccgct ggcgaagatt ggttataaga gtgaagacgt
    19681 cttcgtgccg aagggcgaca aacagcgtcg tttgcataaa gcgttgttgc gttaccacga
    19741 tccggcaaac tggccgttaa tccgccaggc gctggaagcg atgggcaaaa agcatctgat
    19801 tggcagccgt cgcgattgct tagtgcctgc gccaactatt gaagagatgc gtgaggctcg
    19861 tcgccagaac cgcaataccc gtccggcgtt gactaaacat acgccgatgg cgacccagcg
    19921 tcagacgcct gctacggcaa aaaaagcgtc gtctacgcaa tctcgcctgc agaatgctgg
    19981 tgcgaagaaa cgccctaaag cggcggttgg acgttaaaaa atatccccgg taacagaatg
    20041 ctgccgggga tttttttatc attctgcgta catacgcaag cagcgcaggc agtcgaaata
    20101 gtcctgtgca tcaagacgcg tattggctct gttagcttcg gatttggcag catagatttg
    20161 tgggcgtaat gctttcatat cctgctcaag ccgggcgatt tttgcattta atgtttgcac
    20221 attgctcgcc agttgattga ccttttgctc attgacgcag ccactgagca agcaggttgc
    20281 cagaacaaca gctcccacgg tgaaaatcgt tttcatatag tgttcccttt tatttattgt
    20341 tccggctaat tgttatttaa ttcagctcat tgttttaatc tggcggcggt ttttgcctca
    20401 atctgcttaa gtaggctgcc gctgttcggg cggataaaat cagcgatttc ccacttacct
    20461 tcgcgccgct gtaaaatcag ttcttgctct tcgttgttgc cattggtcag cgtaaagcga
    20521 acatgaatgc gggcggaatc ggtggttttc tccaggactc gaatttcatc aacgcgggaa
    20581 aataacgact cttcgccaaa acagatcgga ttgtcgtaaa taaattgccg ttctatttgc
    20641 atatcttcat cgggattgtt ggccgtcttg aaggtggttt tatagaaggc attgaccgcc
    20701 tggcggtaag agtcggaaac cacccacgtc cacggccagc tataatcagc aatatgttca
    20761 aagagttcgg caacaaaggc ttccggctgt tctttttgca acgaagctat agtggccagc
    20821 gttttttcat tctcgctatt aaccgcttgt aagacgctgc catgattgct gacaatatcg
    20881 tcaatgaccc aacgaccatt ttccgccacc attttcagtg tctgcgaggt cttttcttta
    20941 tcgtcttgaa agatacgaaa gcgcacagcg gcatcggcat gatcggcgtc agtttgggtt
    21001 atcgctacgc tttctaacac cagattgcca aaatcctgac aatcacaaac cggatcataa
    21061 tccagccagc caatattgcc cggcagcgta agattgtcgt tcaggataag cgcctgttga
    21121 atacgcgcag aagttatcgc tcgctctccg gtttcaccaa aataaggggc agtggcatct
    21181 gatttatagt tctgataaat atgccggact gtttgttcta cggtctgtga ggggggctgt
    21241 gcgtgtacgg taaaacttgc cagggctgct aaaaacagaa gtattatttt catctgtttt
    21301 tcacctctcc gttgcgttta tcccggatat agatgggttg ttcttttaag agcttccggc
    21361 tctgcatgat gatgtcctta tatttggcat tcctgaacaa ggaagagtag caacgggcgg
    21421 gacgcgaggg gaataaatga tttctgaaaa gtccggtagc ggaacattac cgccaccggg
    21481 ataatttgca cgttagccgc cgaactggtc aggatcggga ccgagacgct tgccctgatc
    21541 gagttttgca atttcgccaa gttcgtcttt gtcgagacgg aaatcccaga catcaaagtt
    21601 ttcgacaatg cgtgaaggtg tgaccgattt tgggatcacc accaggccgc tatccagatg
    21661 ccagcggata acaatctgcg caggggtttt gccgtattta tctgccagat cgcgaatgac
    21721 tttctgatcg aaaacgcctt tccctccttg cgctaatggg ctccaggatt cggtctggat
    21781 tttgtgtgtc gcgttccagg catgtagctg gcgttgttgc atcagcgggt gaagttcgat
    21841 ctggtttatc acaggcgtca cgccagtttc atcaatcagg cgttgcaggt gatggatctg
    21901 gaagttgcac acgccgatgc ttttgattaa tccctctttt tgcaattcga tcatgccttt
    21961 ccatgcttcg acataatggt cgatagcggg aacgggccag tgcattaaat agaggtcgat
    22021 ataatcaagc tggagttttt tcaggctgtc gagcagggct tcgcgggggc gcttgtggtc
    22081 gtcgttccac agcttagtgg tgatgaacag ttcttctctg ttgactgagg catttttcag
    22141 ggctttgccg acaccttctt cgttcttgta ggctgcggcg gtatcaatcg agcgataacc
    22201 cacttctaac gctttttgaa tggcggtgat tacttcctca ttacttgctt gccagacgcc
    22261 cagtcccagc tggggcatga cattgccatc ctgtagctta ataacggttg gattagccat
    22321 acgttcctcc tttatatgaa ctcaccggag caggctccgg tgaggtgttg tgaacttaag
    22381 tctggacgaa atgcccgaaa acgaaagttt gaggcgtaaa aagcttagcg ggcggcttcg
    22441 tatatacggc ggctgacatc cagcgtaatg tcatgatttt cgcccagttg ggtcatgccg
    22501 tgctcttcca gttttttcag caaagccggg atggagctgc cgtccagacc gtagtcggag
    22561 aggtgggtcg gcacgcctaa ttgctcaaag aaattgcggg ttgcggcaat cgcggcgtca
    22621 atacgctcat cgtctgaacc ttcagtgatg ttccagacgc gttcagcata ttgcagcagc
    22681 ttagcgcgct tggtatcgcg tttttcattc cacagtgcag gcaggacgat agccagtgtt
    22741 tgcgcgtgat ccagaccgtg catcgcagtc agttcgtggc ccagcatatg cgttgcccag
    22801 tcctgcggta cgccagcgcc gatcaaaccg ttcagcgcct gagtcgccgc ccacatgacg
    22861 ttggcgcgca catcgtagtt ttctggctct ttcagggctt tcggaccatc ttcgatcagc
    22921 gtcagcaaaa tgccttctgc gaaacggtcc tgaattttgg catcaaccgg tttggtaaca
    22981 tactgttcca cggtgtgtac aaaggcgtcc actacgccgt tagccacctg acgcgacggc
    23041 agggtgtagg tataaaccgg atcgagcacg gcaaatacgg gctgaacatg ggcagaatgg
    23101 aacgcctgct tgtcgcctgt ggttttacgg gagatcaccg cacctgcgtt ggattctgaa
    23161 ccggttgctg gcagcgtcag cacacagccc atcgggatgg cgcttttaat ctctttgccg
    23221 cccgtttgca gaatgtgcca cggatcgata ttttccggat agttagccgc tgcggcgata
    23281 aatttggtgc cgtccagtac agaaccgccg ccaaccgcca gcagaaaagt cactttttgt
    23341 tcgcgaacca gtttcacggc gttcatcagc gtttcataag ccgggtttgg ttcaataccg
    23401 ccaaattcca gcacgtccat gcctttcagg gcatccagaa cttgatcgag aacgccggtt
    23461 tttttcacgc tgccgccgcc gtaggtaatc aatacgcgag catcgtgagg aatttgttcg
    23521 cgtaaaccag cgattgcgcc tttaccaaac agaatgcggg ttggggtgtg cagattaaag
    23581 ttgttcatta cttgctccct ttgctgggtc aatatgaggg cagagatcgg tctgcctgat
    23641 gtttttcatt gtggtcgcca gcgccgtacc tctcaatgct catttctgcc aatgtcttgc
    23701 ctatttctcc agagtgctgg agaaatgcta caaaattgcg cacaatcaaa ttgccgcatt
    23761 attcctaaga aattacgcga tatgaaacgt gaagagattt gccgcttgct ggcggataaa
    23821 gttaataaac tgaaaaataa agaaaatagt ttgtcagagc tgttgcccga tgtgcgtttg
    23881 ttgtatggcg agacgccttt cgcacgcaca ccggtgatgt acgagcctgg catcataatt
    23941 ctcttttccg gacataaaat cggttatatc aatgaacgcg tgtttcgtta tgacgctaat
    24001 gaatatctcc tgctgacggt gccgttgccg tttgagtgcg aaacctatgc cacgtcagag
    24061 gtgccgctgg cagggttgcg tctcaatgtc gatattttgc agttacagga actgttgatg
    24121 gacattggcg aagatgaaca ttttcagccg tcaatggcag ccagcggtat taactccgcc
    24181 acgttatcag aagagatttt atgcgcggca gagcggttac tggatgtgat ggagcgaccg
    24241 ctggatgcgc gcattctcgg caaacagatt atccgcgaaa ttctgtacta cgtgctgacc
    24301 ggaccttgcg gcggcgcgtt actggcactg gtcagccgcc agactcactt cagcctgatt
    24361 agtcgcgtgc tgaaacggat tgagaataaa tacaccgaaa acctgagcgt cgagcaactg
    24421 gcggcagaag ccaacatgag cgtatcggcg ttccaccata attttaagtc tgtcaccagc
    24481 acctcgccgt tgcagtattt gaagaattac cgtctgcata aggcgcggat gatgatcatc
    24541 cacgacggca tgaaggccag cgcggcggcg atgcgcgtcg gctatgaaag cgcatcgcaa
    24601 tttagccgtg agtttaaacg ttacttcggt gtgacgccgg gggaagatgc ggcaagaatg
    24661 cgggcgatgc aggggaatta aatacgttcc cggtacagca accgggaacg gggaaaatcg
    24721 tcaggcgtta cagtattttt ttttaatcac cacaaacagc gtgcccaaca agccagcggt
    24781 taacaaggca attggcagga tcatcaggaa cgtcattacc tgatcttcat gtcgtttaac
    24841 gaacggaatc atacttaagg catagccgaa actggttacc acgctgaccc acagcaatcc
    24901 gcttaaccag ttgaaaaact ggaagcggcg gtttggcaga ccggaaattc ccgccatggt
    24961 tggcagcagc gtacggacaa atgcaagaaa acgtccagcc agcagcgcca gcagaccgtg
    25021 gcggtcaaac atgcaggtgg cgcgctggtg atatttagca ggaagctgtg ccagccagcc
    25081 tttcaccgtt ttggtattcc ctaaccagcg cccctgaata taacttagcc agcagcccag
    25141 acttgctgcg gcggtcagaa tcgcaatcgt aggcagaaaa tccataaccc cctgggcaat
    25201 caatgcgcct gccagtatca acaagctgtc gcctggcaaa aatgaggcgg gcagcaagcc
    25261 gttttctaaa aacagcgtgg caaacataac aaagtaaaca acgctaacaa tatgagggtc
    25321 cgccagcgcg gcaaagtcgt gttgccagag cgcagcgatg atatcttgaa taacagccat
    25381 ggactttcct gtggaacagc gtttaagcct ttatctgcct attgtactcc tgaatcgtcc
    25441 gggacgcctt gatcccggac gcaacaattt aagacttttc acaataaaat gtctgcaaaa
    25501 ttgtccaaaa gtggcaatgt tatacaattc gcgcaaaacc ggcgtccaga tcggcaatca
    25561 gatcgtcgac atcttccaga ccaatatgca ggcgaatcaa ggtcccgcta aaatcgatct
    25621 tgccttgtgg gcgaatggcg gcgatatgtt ctggttgatt tgccaggatc aacgattcat
    25681 acccgcccca cgagtaggcc atgctgaata aactgaagtt atccagatag ttcgccagct
    25741 cttcatcatt gagttttttc ttaagcacaa aggaaaatag cccgctgctg cctgtaaagt
    25801 ctcgtttcca gaattcgtgg cctttactgc caggcagagc agggtggtta actcgcgcta
    25861 cttggggatg ttctgccagc cattcagcca ctttcagact gctttcatga tgttggcgca
    25921 aacgcacacc taatgtgcgc aggccacggc tggttatata ggcggtatcg gcatcgacca
    25981 tctggcccat cagataggca ttctctcgca gctgctccca gcaacgggca ttgcacaccg
    26041 cagtgccaat catcgcatct gaatgcccaa ccagatattt ggtggcggcc tgaatagaaa
    26101 catcgatgcc aaaatccagc gccttaaaca gcacaccggc tgcccaggtg ttgtcgatca
    26161 taatgatggc atccgggacc acactgcgta cggcggcaac aatcgccgga acatcgtgga
    26221 cttccatggt gatggaacct ggcgattcca gaaacacgat tttagtgttt ggctgcagat
    26281 gcttaacgat atcggcacca atcagcggat caaaccacga tgtcgttacg cccagtttgc
    26341 tgaggatttt gctacagaaa tcctgactcg gttcataggc ggtgttggtc atcaacacat
    26401 gatcgccctg ttcgacaaaa gcaagaatgg aattagcaac cgctgccgcc ccgcagggaa
    26461 atagcacaca gcctgcgcca ccttccagtt cacacatcgc ttgttgtaag gagaaatggg
    26521 ttaacgttcc acgccgtcca tagaacaact cgccattggc gcgattacgt gtcgcgtgtt
    26581 ttttggcttc cacactgtca aagaccagcg aagacgcgcg ctgaatcacg ctatttaccg
    26641 caccgagcgt gtattttttg ctgcgtcctg cattcaccag ttgagtatca agctttttgt
    26701 ccgccatgtc gggattcctg tttttatacg tctggatgtc taaactagca tgaatattcg
    26761 cgggcgcatc ctgaagaatt ggcataaagc agaaaatttt cgcaaatggc gtgctggtga
    26821 cttttttact gcgtaaacgc aaggaaaaga aagcaaaggt acggcagtat gcaaatagta
    26881 atgagaacga ctatcaattc gacgtcgttt tgatattatt atgcacagat tttgtgactt
    26941 gcgtcctgga gatacacagt gggtaataat ttaatgcaga cggacctctc cgtctggggt
    27001 atgtatcagc acgccgatat tgtcgttaag tgcgtgatga ttgggcttat tttggcctcc
    27061 gtagtcacct gggcaatctt cttcagtaag agcgtggagt tcttcaatca gaagcgtcgt
    27121 cttaagcgcg agcagcaatt gctggctgaa gcgcgttcct taaaccaggc caacgatatc
    27181 gccgctgatt ttggtagcaa aagcttgagc ctgcatttgc tcaatgaagc gcagaatgag
    27241 ctggaactgt cagaaggcag cgacgataac gaaggtatta aagaacgtac cagcttccgt
    27301 ctggagcgtc gggtcgccgc agtaggtcgt caaatgggcc gtggtaacgg ctatctggca
    27361 accatcggcg cgatttcgcc gttcgttggt ctgtttggta cggtctgggg catcatgaac
    27421 agctttattg gtatcgcgca aacgcagacc actaacctgg cagtcgttgc gccgggtatt
    27481 gcagaagctc tgttagcaac ggcaatcggc ctcgtggcag cgattcctgc ggtcgttatc
    27541 tataacgtat ttgcacgcca gattggcggc tttaaagcga tgctgggtga tgttgcagcg
    27601 caggtattgt tgctgcaaag ccgtgacctg gatctggaag ccagcgccgc tgcacatccg
    27661 gttcgtgtcg cacaaaaatt acgcgcagga taatgtccga tggcgatgca tcttaacgaa
    27721 aacctcgacg ataacggcga aatgcatgat atcaacgtga cgccgtttat cgacgtgatg
    27781 ttggttttgc tgattatctt tatggtggcg gcaccgttag cgacggtaga tgtgaaggtg
    27841 aatttgcctg cttctaccag tacgccgcag ccgcgcccgg aaaaaccggt ttatctgtcg
    27901 gtgaaagcag acaactcgat gtttattggt aacgatccgg tcaccgatga aacaatgatt
    27961 acggcgttga atgcgttaac cgaaggcaag aaagacacca ccatcttctt ccgagcggat
    28021 aaaaccgtcg attacgagac gttgatgaag gtaatggata cgctgcatca ggcgggttac
    28081 ctgaaaatag gtctggtcgg cgaagaaact gcgaaagcga aataaaaatg atgcccggtt
    28141 gcttttcaca accgggcatt tttttaacct aaatgctcgc cgccgcagac accgtgcact
    28201 tctgcggtga cgtagctcga ctcctgactt gccaggtaaa catatacagg ggccagctcc
    28261 gccggttgcc ccgcacgctt catcggcgtt ttctgaccaa actgcgggat cttatcctgc
    28321 gtttgtccgc cagaaatttg cagtgctgtc cagatcgggc ctggcgcgac aatattcacc
    28381 cgaatacctt tctccgcgac ctgttttgcc aggccacggc tatagttcag aattgccgcc
    28441 ttcgtagccg catagtccag taaatgcggg cttggctggt atgcctggat tgaggaagtg
    28501 gtgatgatac ttgcaccttt cggtagcagg gggatcgctt cctgggttag ccagaacaac
    28561 gcgaaaacgt taatggcaaa ggtcttttga aactgttcgc tggtgaggtc tgcaatatca
    28621 ggaatggcaa cctgtttccc ggctaccagc gccataatat ccagcccgcc taacgccttg
    28681 tgtgcttcgt gaaccagcga acgggcgaat ttctcatcgc ttaaatcgcc tggcagcaga
    28741 acggctttgc gtccgcattc ttcaatgatc tttttcacat cctgagcgtc ttcttcttcc
    28801 acgggaagat aactgatcgc cacgtcagcc ccttcacgcg cgtaagcaat ggcggcagcg
    28861 cgaccgattc cggaatcgcc ccctgtcacc agtgctttac gatctttcag gcgaccgcta
    28921 ccaacatagg ttttctcgcc gcaatccggt accggtgtca tcttcgcctg gatgcctggc
    28981 gtcggttgtt tctgtttggg atattcacca gtgtaatact gcgtggtcgg gtcttttaaa
    29041 tgagacatca tttttctccc ttcaggttca acgtccttta agggtagacg ctctcgatgc
    29101 gttgataagg gaaccaggaa gatccctaac cctcagaatt atgcgacaaa ggtttaacgt
    29161 atatgttgat ttgctgttgc gtgctgttta cttaattgcg atatactgtt gctcgtttta
    29221 actacacgac aggaatgtat ggaacgtttt cttgaaaatg caatgtatgc ttctcgctgg
    29281 ctgcttgccc ccgtgtactt tggcctttcg ctggcgttag ttgccctggc gctgaagttc
    29341 ttccaggaga ttattcacgt actgccgaat atcttctcga tggcggaatc agatttgatc
    29401 ctcgtgttgc tgtcgctggt ggatatgacg ctggttggcg gtttgctggt gatggtgatg
    29461 ttttccggtt acgagaattt cgtctcacaa ctggatatct ccgagaacaa agagaagctg
    29521 aactggctgg ggaaaatgga tgcaacgtcg ctgaaaaaca aagtagcagc gtcgattgtg
    29581 gcaatttctt ccattcactt actgcgcgtc tttatggatg cgaaaaatgt cccggataac
    29641 aaactgatgt ggtacgtcat tatccatctg acgtttgtgc tctctgcatt tgtgatgggc
    29701 tatcttgacc gactgactcg tcataatcac tgatctgatt cgggcgcggt tctcgcgccc
    29761 gttattaaca ggtcatttat cggaagacgc ctgccacaga ttcagctcgc catcggcgat
    29821 atgctgatca atctgcgcca gctcctcggt gctaaatgtc agattattca gcgcctgcac
    29881 gttctcctca agttgctccg cgcggctggc accaatcaat accgacgtca cgcgatcatc
    29941 tttcagcaac cagcttaacg ccatttgcgc cattgattgt ccacgctgct gtgccatttc
    30001 attcaataac cgcaggctgt tgaggttggc ttcggtgagc attttcggcg tcagaccacg
    30061 aactttattc ccttcacgat gcatccgtga atcttgcgga ataccgttga gatattttcc
    30121 ggtcagcaat ccctgtgcca gtggagtaaa ggcaatgcag cccacgccgt tattttgcag
    30181 ggtatccagc aggccgcttt tatccaccca gcggttcagt aaattgtacg aaggttgatg
    30241 aattaacagc ggaattttcc actcgcgcag caactcgacc attttttgcg tccgctctgg
    30301 cgagtaagag gagatcccga catacagcgc cttaccgctt tgtacagcat gagccagcgc
    30361 agaggcggtt tcttccatcg gcgtattttc atcgacgcga tgagagtaaa agatatcgac
    30421 atactcaagc cccatacgct tcaggctttg gtcgaggctg gcgagcaggt atttacgtga
    30481 gccgccagag ccgtatgggc cgggccacat atcgtatccg gctttggtag agataatcag
    30541 ttcatcgcga taagcggcaa aatcctcgcg cagcaggcga ccaaagttct cttctgcgct
    30601 tcctggaggc ggcccgtagt tgttggctaa atcaaagtgc gtaatgccta aatcaaacgc
    30661 tttacgcagg atcgcacgct gtgattccag cgcgttaacg tgaccgaaat tgtgccataa
    30721 accgagcgat aacgcgggca ggcgtaaacc actttttccg caatagcggt actgcatctg
    30781 cccgtaacgt tcgggattcg ctaaccagac catgacctct cctttccatc gttcaatttc
    30841 gaaacaatgt ttctagttta gcgattcgcc agcgcgtatc ccgtagtctg gctcacagag
    30901 tgacgaaaaa ctgggcaaaa acacgcgctt atgctttgct taaaaaaaca ccagttgagg
    30961 agtgcaacga tgccgcgttt aaccgccaaa gatttcccac aagagttgct ggattactac
    31021 gactattacg ctcacgggaa aatctcgaaa cgtgagttcc tcaaccttgc ggcgaagtat
    31081 gcggtgggcg ggatgacggc attagcgctg tttgatttgc tcaagtcaaa ttatgcgttg
    31141 gcgactcagg tagagtttac cgacccggaa attgttgctg agtacatcac gtatccctcg
    31201 ccaaatggtc acggcgaggt acggggttat ctggtgaagc ccgcaaaaat gagcggcaaa
    31261 acgccagccg tggtagtggt gcatgagaat cgagggctga atccgtatat cgaagatgtg
    31321 acgcggcgag tggcgaaggc tgggtacatc gccctggcgc cagacggttt aagctcagta
    31381 ggtggctatc ctggtaacga tgataaaggc cgcgaactgc aacagcaggt tgatccaacc
    31441 aaactgatga atgatttctt tgccgcaatt gagtttatgc aacgctatcc gcaagcgaca
    31501 ggcaaagtgg gtattagcgg attttgctat ggcgggggcg tatcgaacgc ggcagcagtc
    31561 gcgtatccgg aactggcctg cgcggtgccg ttttatgggc gtcaggcacc cactgccgat
    31621 gtggcgaaga ttgaagcgcc tttactgctc cactacgctg aactggatag ccgaatcaac
    31681 gagggctggc ccgcttacga ggcggcgttg aaagccaata ataaggtcta tgaggcgtat
    31741 atatatccgg gggttaatca cggattccat aatgattcca cgccccgtta tgacaaatct
    31801 gccgccgatc ttgcctggca aaggacgctg aaatggttcg ataaatatct ctcctgatgg
    31861 gtttatctct tacaggatta cgtcttaaac aagcatgaaa aaatagcgtg cgcaaatgtc
    31921 gttctttgcc taaaatatcg ctatatatga caatatatag cgaatgaggt gaacgatgaa
    31981 taaccatttt ggtaaaggct taatggcggg attaaaagca acgcatgccg acagtgcggt
    32041 taatgtgaca aaattctgtg ccgattataa acgcggtttt gtattaggct actcacaccg
    32101 gatgtacgaa aagaccggag atcgccagct tagcgcctgg gaagcgggta ttctgacgcg
    32161 ccgttatgga ctggataaag agatggtaat ggatttcttt cgtgagaata attcctgttc
    32221 tacgttgcgc ttttttatgg ccggttatcg cctagaaaat tgatcaaaca tacgtattat
    32281 cttgctttaa ttaattacac taatgcttct tcccttcgtt ttagtgcccc gccgcagtat
    32341 catgatatcg ataaccataa taaatgtgtg gtaaatggcg catcgatcgc attattgatt
    32401 ttgcgattga ggcaaaatat atgccaggtc ttcgcaacgg aataactata aatgactgga
    32461 gataacaccc tcatccattc tcacggcatt aaccgtcgtg atttcatgaa gctttgtgca
    32521 gcattagccg ccaccatggg gttaagtagc aaagccgctg cagagatggt cgaatcggtt
    32581 actaacccgc agcgtccgcc agttatctgg attggcgcgc aggagtgcac cggttgtacg
    32641 gaatctctgc ttcgtgcaac gcatccaacg gtagaaaacc tcgtgctgga gactatctct
    32701 ctggagtatc acgaagtgct ttccgccgcc ttcggtcatc aggtcgaaga gaacaaacat
    32761 aacgctctcg agaagtacaa agggcagtat gtgttagtgg tggatggttc catcccatta
    32821 aaagataacg gtatttattg catggttgcc ggtgagccga ttgtggatca catccgcaaa
    32881 gcggcggaag gcgcagcagc cattatcgct atcggttcct gctctgcgtg gggcggtgtt
    32941 gccgcagctg gagttaaccc aactggcgca gtcagcctgc aagaagttct accgggcaaa
    33001 accgttatca atattcctgg ctgcccgccg aacccgcaca acttcctggc gaccgttgcg
    33061 cacatcatca cttacggcaa accgccgaaa ctggatgaca aaaaccgtcc gactttcgcc
    33121 tatggtcgtc tgattcacga acactgtgaa cgtcgcccgc acttcgatgc tggtcgtttt
    33181 gccaaagagt tcggtgatga aggccaccgc gaaggctggt gcctgtacca cctcggctgt
    33241 aaagggccag aaacttacgg caactgctca acgctgcaat tctgcgatgt tggcggcgtg
    33301 tggccggtag cgattggtca cccgtgctat ggctgtaacg aagaaggtat cggcttccat
    33361 aaaggcatcc atcagcttgc caacgtcgaa aaccagactc cgcgttcaca gaaaccggac
    33421 gttaacgcta aagaaggcgg caacgtctcg gcaggcgcta ttggtttgct cggcggagtg
    33481 gttgggctgg ttgccggtgt cagcgtgatg gcggtgcgtg aactgggtcg tcagcaaaag
    33541 aaagataacg ctgactcacg gggagaataa ccgtgaacag acgtaatttt attaaagcag
    33601 cctcctgcgg ggcattgctg acgggcgcgc tgccgtctgt cagtcatgcg gctgctgaaa
    33661 accgcccgcc aattccggga tcgctgggga tgttgtacga ctcgaccttg tgcgtaggct
    33721 gtcaggcttg cgtcaccaag tgtcaggata tcaactcccc tgaacgtaac ccgcaagggg
    33781 aacagacctg gtcgaacaac gacaaactgt cgccgtatac caataacatc attcaggtgt
    33841 ggaccagcgg cacaggggtc aacaaagacc aggaggagaa tggctacgcg tacattaaga
    33901 aacagtgtat gcactgcgtc gatccgaact gtgtctctgt gtgcccggtc tctgcgctga
    33961 aaaaagatcc gaaaaccggc attgtccatt acgacaaaga tgtgtgcacc ggctgccgtt
    34021 actgcatggt cgcctgccca tacaacgtgc cgaagtacga ctacaacaac ccgtttggtg
    34081 cgctgcataa gtgcgagttg tgcaaccaga aaggtgtgga acgtctcgat aaaggcggtc
    34141 tgcctggctg cgtagaagtg tgcccggcgg gcgcggtgat ttttggtacg cgtgaagagc
    34201 tgatggcgga ggcgaaaaaa cgtctggcgc tgaagcctgg cagcgaatac cactatccgc
    34261 gtcagacgct gaaatctggc gacacttacc tgcatacggt gccgaaatat tatccgcatc
    34321 tgtacggcga gaaagagggc ggcggtactc aggttctggt actgacgggt gtgccttatg
    34381 aaaatctcga cctgccgaaa ctggacgatc tttctaccgg tgcgcgttcc gaaaatattc
    34441 aacacaccct gtataaaggc atgatgctac cactggctgt gctggcgggc ttaaccgtgc
    34501 tggttcgtcg caacaccaaa aacgaccatc acgacggagg agacgatcat gagtcatgat
    34561 ccacaaccgc tgggcggcaa aatcatcagt aaaccggtca tgatttttgg accgttaatc
    34621 gtcatctgta tgctcctgat tgtgaagcgt ctggtgttcg gtctgggctc tgtctctgac
    34681 ctgaacggcg gcttcccgtg gggcgtgtgg atcgcgtttg acctgttgat cggcaccggc
    34741 tttgcctgtg gcggctgggc gctggcgtgg gcggtatacg tctttaaccg tgggcaatac
    34801 catccgctgg tgcgtccggc gctgttggcg agtctgtttg gttactcact aggtggcttg
    34861 tcgatcacta tcgacgtggg tcgctactgg aacctgccgt acttctacat tccgggtcac
    34921 ttcaacgtga actcggtact gttcgagacg gcggtctgta tgaccatcta tatcggcgtg
    34981 atggcactgg agtttgctcc ggcactgttt gaacgtctgg gctggaaggt gtcgctacag
    35041 cgtctgaaca aggtgatgtt cttcatcatc gcgctcggtg cgctgctgcc gaccatgcac
    35101 cagtcttcaa tggggtcgct gatgatctcg gcgggctaca aggtgcatcc gctgtggcag
    35161 agctatgaaa tgttgccgct gttctcgctg ctgacggcgt tcatcatggg cttctcgatt
    35221 gtcatctttg aaggttcact ggtgcaggcg ggtctgcgtg gcaacggtcc ggatgaaaag
    35281 agcctgttcg tcaagctgac caacaccatc agtgtgttgc tggcgatttt catcgtgctg
    35341 cgctttggcg agcttatcta tcgcgacaag ctgtcgttag cgtttgccgg tgacttctac
    35401 tccgtgatgt tctggattga agtcctgctg atgctcttcc cgctggtcgt tctgcgtgtg
    35461 gcgaagctgc gtaatgattc ccgcatgctg ttcctgtcag cactgagcgc actgttaggt
    35521 tgtgcaacct ggcgtctgac ctattcgctg gtggcattca acccgggcgg cggttacgcc
    35581 tacttcccga cctgggaaga actgttgatt tctattggtt ttgtggctat tgagatttgc
    35641 gcttacatcg tactcattcg tctactgccg atacttcctc ctttaaaaca aaacgatcat
    35701 aatcgtcatg aggcgagcaa agcatgagcc agagaattac tattgatccg gtaacccgta
    35761 ttgaggggca tttacgcatc gattgcgaaa tcgaaaatgg cgtcgtttcg aaagcatggg
    35821 cttccggtac catgtggcgc ggcatggaag agatcgtgaa aaaccgcgat ccgcgcgatg
    35881 catggatgat tgtgcaacgt atctgtggcg tatgtactac cactcacgcg ctgtcttccg
    35941 ttcgtgcggc agaaagtgcg ctgaatatcg acgttccggt taacgcgcaa tacatccgta
    36001 acatcattct ggctgcgcac accacgcatg accatatcgt tcatttctat cagctttcgg
    36061 cgctggactg ggtggacatc acttctgcac tgcaagctga cccaaccaaa gcctctgaaa
    36121 tgctgaaagg cgtttcgacc tggcatctga acagtccgga agagttcacc aaagttcaga
    36181 acaagattaa agatctggtt gccagcggtc agttgggtat tttcgccaat ggctactggg
    36241 gtcacccggc gatgaaactg ccgccggaag tgaacctgat tgcggtagcg cactacctgc
    36301 aggcgctgga gtgccagcgt gacgctaacc gcgtcgtggc gctgctgggc ggtaaaacgc
    36361 cgcacattca gaacctggcg gtaggcgggg tcgctaaccc aatcaacctc gacggtttgg
    36421 gcgtgctgaa ccttgagcgc ctgatgtaca tcaagtcttt catcgacaag ctgagcgact
    36481 ttgttgaaca ggtttataag gttgataccg cagttatcgc cgcgttctac ccggaatggc
    36541 tgacgcgcgg taaaggtgcg gtgaactacc tgagcgtgcc ggaattcccg accgacagca
    36601 aaaacggcag cttcctgttc ccgggcggct acattgagaa tgcggatctg tcctcgtatc
    36661 gtccgatcac ttctcattcc gatgaatacc tgatcaaagg cattcaggaa agcgcgaagc
    36721 actcctggta taaagacgaa gcgccgcagg caccgtggga aggtaccacc attccggctt
    36781 acgacggttg gtctgatgac ggtaaatatt cctgggtgaa atcgccgact ttctacggca
    36841 aaacggtaga agtgggtccg ctggctaata tgctggtgaa actggcggca ggtcgcgaat
    36901 ctacccagaa caaactgaat gaaatcgttg cgatttatca gaaactgact ggcaacacgc
    36961 tggaagtggc acagctgcac tccacgctgg gccgtattat tggtcgtacc gttcactgct
    37021 gtgaattgca ggatatcctg caaaaccaat acagtgcact gatcaccaat atcggcaaag
    37081 gcgatcacac cacctttgtg aagccgaaca ttccggcaac gggtgagttc aaaggtgttg
    37141 gcttcctcga agcgccgcgc ggtatgctct ctcactggat ggttattaaa gacggtatca
    37201 tcagcaacta ccaggcggtt gttccatcaa cctggaactc tggtccgcgt aacttcaatg
    37261 atgacgtcgg tccttacgag cagtcgctgg tgggtacacc ggttgccgat ccgaataaac
    37321 cgctggaagt ggtgcgtacc attcactcct tcgacccgtg catggcctgt gcggtacacg
    37381 tagtggatgc cgacggcaac gaagtggttt cagtgaaggt tctgtaatgc gtattttagt
    37441 cttaggggtc ggcaatattt tgctgaccga tgaagccatc ggtgtgcgga ttgttgaagc
    37501 gttagagcaa cgatacattc tgccggatta tgttgagatc ctcgatggcg gcacggcggg
    37561 aatggagctg cttggcgaca tggcaaatcg cgatcatctg attattgcgg atgccattgt
    37621 gtcgaaaaag aacgcgccgg gaacgatgat gatcctgcgg gatgaagaag ttccggcgtt
    37681 gtttaccaac aaaatctctc cgcatcagct tggcctggcc gacgtattgt cggccctgcg
    37741 cttcaccggc gagtttccga aaaagctgac gctggtcggt gtgatcccgg aatcgctgga
    37801 gccgcacatc ggcttaacgc cgacggttga agcaatgatt gaacctgcgc ttgagcaggt
    37861 tctggctgcg ctgcgcgaat caggcgtgga agctatccca cgggaggcga ctcatgactg
    37921 aagagatagc aggtttccag acctccccga aggcgcaagt acaggcagcg tttgaagaaa
    37981 ttgcccggcg ttcgatgcac gatctctctt ttctgcatcc ctcaatgccg gtgtatgttt
    38041 ctgactttac gctgttcgaa ggtcagtgga cggggtgtgt gatcaccccg tggatgctga
    38101 gtgcagttat cttccccggc ccggatcaac tctggccgct gcgcaaagtg agtgaaaaaa
    38161 ttggtctgca actgccgtat ggcactatga cctttaccgt tggcgaactg gacagtgttt
    38221 ctcaatatct ctcctgttcg ctgatgtcac cgctttcgca tagcatgtcg attgaagagg
    38281 gccaacgcct gacggatgac tgcgcgcgga tgatcctttc gctaccggtc gctaatccgg
    38341 atgtaccaca cgcagggcgt cgcgccctgc tgtttggtcg caggagtggc gaaaatgcat
    38401 gagttgtcgc tttgccagag cgccgttgaa attatccaac ggcaggcgga gcagcacgat
    38461 gttaagcgcg tcaccgccgt gtggctggaa attggcgcgc tctcctgcgt tgaggagagc
    38521 gccgtccgtt ttagttttga aattgtctgc cacggaacgg tggcgcaagg gtgcgattta
    38581 catatcgtct ataaacccgc ccaggcctgg tgctgggatt gcagccaggt ggtggagatt
    38641 catcagcacg atgcgcagtg tccgctctgt catggcgagc ggttgcgtgt cgataccggc
    38701 gattcgctga tcgtcaaaag tattgaagtt gaataactgg agttaacaat gtgtattggc
    38761 gttccaggcc aggtgctggc tgtcggtgaa gatattcacc agcttgcgca ggttgaagta
    38821 tgtggtatca agcgcgacgt gaatatcgcc ctgatttgtg aaggtaaccc tgccgatctg
    38881 ctgggccagt gggtgctggt acacgtcgga tttgccatga gcatcatcga cgaagatgaa
    38941 gccaaagcca cattagacgc actgcgccaa atggattacg acattaccag cgcgtgatga
    39001 ttaaagccgg atgcggcgta aatgctttat ccggctacat agcgataagc ctgccaatgt
    39061 tgcgacttac ccctgacgct tatcttccgt attcgtctcg aaatcactgg cgtcatggcg
    39121 ctcatgcaac tgctcgttca gcggcccgtt ggtgcggtta acaatacgcc cacgcttcac
    39181 cgccggacgt tcgcccactt ctttcgccca gcgttgcacg tgcttataac tgcccgcatc
    39241 cagaaactct gcggcatcat acacgccgcc taacaccacg ttgccaaacc acggccagat
    39301 cgccatatcc gcaatggtgt actcatcgcc cgcaacaaac ttgtgctgcg ccagttgctt
    39361 atccagcacg tcgagcaggc gcttggcttc catggtaaag cggttgatgg cgtactcaat
    39421 tttcaccggc gcgtaatggt aaaagtgacc aaaaccaccg ccgaggaacg gtgccgcgcc
    39481 ctgtaaccag aacagccagt tcatcgtttc agtacgcttt gccaaatcct gcggcaggaa
    39541 gtagccaaat ttctccgcca gataaagcag aatcgatcca gactcaaaca cgcggatcgg
    39601 tggattatgg gtatggtcgc gcagcgccgg gatcttcgag tttgggttca cttcgacaaa
    39661 gccgctggag aattgatcgc catcgccaat acgaatcagc caggcgtcgt actctgcacc
    39721 agtaacgccc agtgccagca gctcctcaag cataatcgtt actttctgac cgttcggcgt
    39781 tcccagcgaa taaagttgca atggatgttt gcccaccggc agcgtttttt catgcgtcgg
    39841 accagaaacc gggcgattga tactggcgaa cgcgccgcca gcggatttat cccacgtcca
    39901 gactttcgcg ggctgataag tattgtctgt catagtgagt tgccttctga gtggtagtgt
    39961 tgaagcagtg tagcaggtca cttgctgtac agaatgtgaa ttttttaacc ctttgcgcca
    40021 gccgctgcga tgaggtgtat gattagtctg acctctgtcc ggttaatgtc gctgagcagg
    40081 taatcagagc acaacaatta cgccaactgc ttaggttgta ttgttaaagg taaagtgatg
    40141 agcaaaggaa cgaccagcca ggatgccccg ttcgggacat tattgggcta cgccccaggt
    40201 ggggtagcaa tctactcttc agattacagt tctctcgatc cgcaggaata cgaagatgac
    40261 gccgtattcc gtagctatat cgacgatgaa tatatgggcc acaaatggca atgcgttgaa
    40321 tttgctcgcc gttttctctt tctgaactac ggtgtagtct tcactgacgt gggtatggca
    40381 tgggagattt tctcactgcg cttcctgcgt gaagtggtca atgacaacat cctgccattg
    40441 caggcatttc ctaacggttc gccgcgtgca ccggtagcgg gggcgcttct tatctgggat
    40501 aaaggcggtg aatttaaaga cactggccat gtcgccatca ttacccaatt gcatggcaac
    40561 aaagtccgta ttgcggaaca gaacgtgatt cataccccgt tgccgcaagg gcaacagtgg
    40621 acacgcgagc tggagatggt ggtcgaaaac ggcggctata ccctgaaaga caccttcgat
    40681 gacaccacta ttctgggctg gatgatccag acggaagata ccgaatacag cttgccacag
    40741 ccggaaattg caggcgaact gctgaaaatc agcggggcgc gtctggaaaa caaaggccag
    40801 tttgacggta aatggctgga tgaaaaagat ccgctgcaaa acgcctatgt acaggccaac
    40861 ggccaggtga tcaatcagga tccgtatcat tactacacca ttaccgagag tgccgagcag
    40921 gagctgatta aagccaccaa cgagctgcac ctgatgtatc ttcacgccac cgacaaggtg
    40981 ctgaaagatg acaacctgct ggcgctgttt gacattccga aaatcctctg gccacgtttg
    41041 cgtctctcct ggcagcgtcg ccgtcatcat atgatcactg gtcgtatgga tttctgcatg
    41101 gatgaacgtg gcctgaaagt ctacgaatac aacgccgatt ctgcctcctg tcataccgaa
    41161 gcgggcttga tcctcgaacg ttgggcggag cagggctata aaggcaacgg cttcaatcct
    41221 gcggaaggac tgattaacga actggctggc gcgtggaaac acagtcgtgc acgtccgttt
    41281 gtccatatca tgcaggacaa agatatcgag gaaaactatc acgcgcagtt tatggagcag
    41341 gcgctgcacc aggcgggctt tgaaacgcgt atcttgcgtg ggttggatga actgggctgg
    41401 gatgctgccg ggcaactgat tgatggggaa gggcgactgg ttaactgcgt atggaaaacc
    41461 tgggcgtggg aaacggcatt tgatcaaatc cgcgaagtga gcgaccgtga atttgccgca
    41521 gtgccgatcc gtaccgggca tccacaaaat gaagtgcgtc ttatcgacgt attgctgcgc
    41581 ccggaagtgt tggtttttga gccgctatgg acggtgatcc ccggtaacaa agcgattctg
    41641 ccgatcctct ggtctctgtt cccgcaccat cgttatctgt tggatactga cttcaccgtg
    41701 aatgatgagc tggtgaaaac cggttatgcc gttaaaccga ttgccgggcg ctgtggtagc
    41761 aacattgacc tggtcagcca tcatgaagag gtgcttgatc agaccagtgg taaatttgcc
    41821 gaacagaaaa atatttacca gcaactgtgg tgtttgccga aagtggacgg taaatacatt
    41881 caggtatgta ccttcaccgt tggcggcaac tacggtggga cgtgtttgcg cggtgatgaa
    41941 tcactggtca tcaaaaaaga gagtgatatt gaaccgttaa ttgtggtgaa aaagtaatac
    42001 atgttgtagc aaccccgtac tgattatgaa gaataatcag tacggggatt aatcaaaaca
    42061 ttcaatattt atatattcca atatttattc attttatatg tgaatatatt aaccagcaga
    42121 atacctgtgt tttgattatt tctaaaggtt ttgaatgaat gcttattgtc tgatacacaa
    42181 gaaataacac gctttttatc gttaaaaaat gatatttcac tttgcccatg ccgttaaagt
    42241 cactgataat gcgtccgttc gtaaattcaa aatggcgtaa tctaatatat gctaaattta
    42301 tttgttggcc ttgatatata cacagggctt ttgttattac ttgctctggc atttgtgttg
    42361 ttctacgaag caatcaatgg ttttcatgac acggcgaatg cggtggcaac cgttatttat
    42421 actcgtgcca tgcaaccaca acttgctgtg gtgatggcgg cattttttaa tttttttggc
    42481 gtgttattgg gcggacttag cgttgcctat gccattgtcc atatgttacc aaccgatttg
    42541 ttgctgaata tggggtcaac tcacggcctg gcgatggtct tttccatgct gctggcggcg
    42601 attatctgga acctgggaac gtggttcttt ggtttaccgg cctccagttc gcacaccttg
    42661 attggtgcga ttatcggcat cggtttaacc aacgcgctgt taaccggctc atcggtgatg
    42721 gatgcgttaa acctgcgtga agtgaccaaa attttctcct cgctgattgt ttcccctatc
    42781 gtcggcctgg tcattgcggg tggtctgatt ttcctgctgc gccgtttctg gagcgggacg
    42841 aaaaagcgtg accgtattca ccgcattccg gaagatcgca aaaagaaaaa aggcaaacgt
    42901 aagccgccat tctggacgcg tattgcgctg attgtttccg ctgcgggcgt ggcgttttcg
    42961 cacggcgcta acgacgggca gaaagggatc ggcctggtaa tgctggtact ggtggggatt
    43021 gcccctgctg gcttcgtcgt caatatgaac gcgtccggct atgaaattac ccgtacccgc
    43081 gatgccgtca ccaacttcga acactacctg caacagcatc ctgaactgcc gcagaagttg
    43141 attgcgatgg aacctccatt gcctgcggca tcgactgatg gcgcgcaagt gacggagttt
    43201 cactgtcatc cggcaaatac ctttgatgcg attgcgcgcg ttaaaacgat gctgccaggc
    43261 aatatggaaa gctacgagcc gttaagcgtg agtcagcgca gccagctgcg ccgcattatg
    43321 ctgtgcatct ctgatacttc cgcgaagctg gcgaaactgc caggcgtcag taaagaagac
    43381 cagaacctgc tgaaaaaact gcgcagcgat atgttaagca ccattgagta cgcaccggta
    43441 tggatcatca tggcagtagc actggcgctc ggcattggca ccatgattgg ctggcgtcgt
    43501 gtagcgatga ccatcggtga gaagattggt aagcgcggca tgacgtatgc gcaaggcatg
    43561 gcggcacaaa tgacggcggc agtgtctatc ggccttgcca gttatattgg gatgcccgtc
    43621 tccacaacac acgtactctc atctgccgta gcaggaacga tggtggtgga cggcggtggg
    43681 ttacagcgta aaacggtaac cagcatcctg atggcgtggg tgtttacttt accggcggcg
    43741 atttttcttt ctggtgggtt gtactggata gcattgcagt tgatttaacc ttcctgaaaa
    43801 tgcccggtcc tggcgatcgg gcatttccat ttttgactag tgataaccac gcgcggtcat
    43861 aaaatccgta atcgcttttt ctgcatcaat cagcaccttt tccagtggct gattagcatc
    43921 gatatcaacc agttgtgcac cgccaaaggt taactgtggc gttatggcaa tcttcctcgc
    43981 cagcgattcc cgtttatggt cgggtttacg cgcgcaggca acttcaaggt caacattgag
    44041 tttgatgacc agatcaggtt tatggctcgc catccagtga aacgctttac gttcctggct
    44101 tgccagccat gagacaaaac gaccaccttc aatgttaggt gggaacactg taccatcgta
    44161 agcgccagga atttggtcct gaggataacg gtcggttaga acaattaacc cgcgacgacg
    44221 acaggcaagc atatgacgaa agcgcagtaa gcgacgggcg acaaacgctg taattaccag
    44281 cgccggaact ggtcctggca atttttttgc tgttttcacc tgatttcgtt caattgtttt
    44341 atgtaaggat tttcccatca acggtaattt tgtcactgaa cgaccgacat ttccggcctg
    44401 ttttcctaaa tgaactcttt cggcagcacc atatttttcg acaacggtaa tgagatgttc
    44461 acacaccgtt gacttgcctg aaccatcgct accaataacg gcaattaatg gaggtgttat
    44521 tgactgcatg aatatatcct ttaatttaaa tatccattaa aaatatttat ttggttaata
    44581 tgtttttatg aaagcgtaat tcaggtcaat gtcacaatta accattgtca caataaggtt
    44641 gaacggatat tctgagcacg gaccacgtaa tattcaatac attatttgct gccgggtttt
    44701 tcgtgaatca acgtaatatg tcaataatta attccacgcc agtgcgtgtt attgccattg
    44761 taggatgtga tggttcaggt aaatcgaccc tcacggcaag cctggtaaat gaactggcag
    44821 caacaatgcc aacagaacac atttatctcg ggcaatcgtc cgggcgaatt ggcgaatgga
    44881 tttcacagct ccctgttatt ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac
    44941 atgtgcacga aaagccctcc acaccgcctg gcaatatgac tgcgctggtt atctatctgc
    45001 tttcctgctg gcgggcgtat aagtttcgca aaatgttgtg taaaagccag caaggctatc
    45061 tgctcatcac cgaccgctac ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc
    45121 aattggcaaa aaccacgggc ggtaacggtt ggataaaaat gttaaggcag cgcgagctga
    45181 agctgtacca atggatggca tcttatttgc ccgtattgtt gattcgtctt ggcattgatg
    45241 aacaaaccgc gtttgcgcgt aaacctgacc accaactggc ggcgttacag gaaaaaatcg
    45301 ccgttacgcc gcaactgaca tttaatggcg ccaggatcct tgaactggat ggtcggcaac
    45361 cagccgatga aattctgcaa gcgtcactac gcgcaattca agctgcgctt tcctgatcac
    45421 caaaatggat aggggatgat ttaacgaatg gacgcactac aaacacaaac tgttaatagt
    45481 acaaccgcgc cgcagcccaa ctacattccg gggctgattg cggtggtcgg gtgtgatggc
    45541 accggtaaat ccacactgac caccgacctc gtgaaatcgc tgcaacaaca ctggcaaacc
    45601 gagcggcgct atctggggct gctctcgggc gaagacggcg acaaaatcaa acgattgccg
    45661 ttggttggcg tctggctcga aaggcgactg gcggccaaat catcgaaaac ccaaagcatg
    45721 aaaacccaaa gcatgaaaac caaatctcca gcgctatggg cggcggtgat tatgtactgc
    45781 ttctcgctgc gaagaatggc gaatctacgc aaggttcagc gactggcgca aagtggcgtt
    45841 ctggtggtca gcgatcgctt cccgcaggct gaaatttcgg gcttttatta tgacggaccg
    45901 gggatcggcg tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg
    45961 cgtttatacc aaaaaatggc gcaataccgt cctgaattaa tcattcgttt ggggattgat
    46021 attgatactg ccatctcccg caagcctgac catgactatg ccgagctgca ggacaaaatc
    46081 ggcgtcatgt cgacgattgg ctataacggc acaaaaattc ttgagattga ttccagagcg
    46141 ccctacagcg aagtgctgga acaggcacag aaggcggttt ctctggttgc catcgtttct
    46201 gaccgccgaa gtttaactta aggctggcgg gatacttttt actatcaacg ataaggtcag
    46261 gctgaattgg cgggttttaa catcaaacat tggtttgcag atggcgcgtt tcgcaccatt
    46321 attcgcaata gcgcctggtt aggctccagt aatgtcgtga gcgctttact gggtctgttg
    46381 gcgctctcgt gtgccggtaa agggatgacg cccgccatgt ttggcgtact ggtgattgta
    46441 caatcgtacg ccaagtcgat cagcgatttt attaagtttc agatatggca gctggtggtt
    46501 cagtacggaa caccagcatt aaccaacaat aatccgcagc aattccgcaa tgtcgtctca
    46561 ttttccttct cgctggatat cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta
    46621 ctcccttttc tttcccattc attaggtctg gatgaccaaa gcttttggct ggcagcgctg
    46681 tattgcacgc tcattccttc aatggcttcc tccacgccga ccggcattct gcgtgcggta
    46741 gatcgcttcg atctaattgc tgtacagcaa gcgacgaaac cttttctgcg cgcagcgggg
    46801 agcgtcgtag cctggtattt tgactttggt tttgcgggtt ttgttattgc ctggtacgtg
    46861 tcgaatctgg ttggcggcac catgtactgg tggtttgccg cgcgcgaatt acgccgcaga
    46921 aatatccata acgccttcaa attgaatctg tttgagtctg cccgacacat taaaggcgcg
    46981 tggagttttg tctggtcaac caacattgcc cactccatct ggtcggcgcg taactcgtgc
    47041 agcacagtgc tggtggggat cgtgttagga cccgctgccg ccgggttatt taaaatcgcc
    47101 atgacattct tcgacgccgc cggaacgcca gcgggtttac tgggcaaaag tttttaccca
    47161 gaggtgatgc gtttagatcc gcgcaccacc agaccgtggt tgttaggcgt gaagtcaggt
    47221 ttactggcgg gcggaattgg tattctggtg gcgctcgcgg tgttgatcgt cggcaagccg
    47281 cttatttcgc tggtgtttgg cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg
    47341 ctgggcgcaa ttgtgatttc gatgctgggc ttcccacagg agtcattgct attgatggcg
    47401 ggaaaacagc gcgcttttct cgtggcgcaa accatcgcct caatcggcta catcgtattg
    47461 ctgttcatgt tctgtcatct gtttggcgtg ctgggagcag cgtttgctta tttcggtggg
    47521 caatgtctgg atgtggtgct ctcgttaatt ccgaccttaa aggcattttt tcagcgccat
    47581 tccttgcttt ataacgcagc cggagagaaa tcctgatgat gagaaaatat ttcccgcttg
    47641 aggcgagcga gcgtttattt gttgcagttg aagaagatga tgtcgttgat gcacaggtca
    47701 gtttgccgcc gactatcgca ctaagttgta ccaccgaaat catccatgat aattacgcgt
    47761 tatgcctgca attctggctc aatggcgtca atcgccagga gttactgcgt ttaatatgca
    47821 aacaggccaa aggcgatgag ctgactgctg atgagcgcaa acaatttaaa tacatgcgag
    47881 cgcgctacaa gcacctgcgt tttgcgcaac ggctgtattt aaagaagcat caggccggat
    47941 ttttattcgg caaaacgacc gtttttttgg ggcgttttca ggacggtttt cgtaacggta
    48001 agaaaaatat tgtgtcgtat tacggcaact tgttacgggt ttatttaagc tctccggtct
    48061 ggtcgttggt gaactattca ttacgccata gccagttaga aagcgtaagc ggttttattg
    48121 cctatcggca aaagcaaatg cataccctga aagagataat tgccaaaccg cgactcaccg
    48181 gcagggaatt tcatgatgtg cgaaaaatca tcagccagca ggtttcttat tacgacacct
    48241 taagatcgct tgatcctgaa aataaagagg cgttacagat ttcccgcttc ctcgccgcca
    48301 ttaatggcct gatgggggat aaacacgacg acatggtggc ggatgatatg gaaaaccgcc
    48361 agtcgtatga tgcaccggtg gcgctggata gcgatattcg ccagcgctta gagctgctga
    48421 tttcgcgttt tccgctataa ccggagcaat gatgtcatct cgtttgatta ttgcgctgat
    48481 tatcatgctc ttagcccagg gtgtgcaggc acacaatttt gtcactggca aaacggttac
    48541 ccccgtttat atccaggaag gcggcgagct gcttctgaat agcgacgacg aaattcacta
    48601 ccaaaagtgg aacagcacac agctggcggg aaaagtccgc attatccagt atatcgccgg
    48661 gcgtaagtcg gcgaagaaaa agaactcact gttaattaaa gcggttgaag cggcaaactt
    48721 tccgcaggat cgttttcagc ccaccaccat tgtgaacacc gatgatgcca tctttggtac
    48781 tggctatttt gtcgttggca aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt
    48841 cgttattgac ggcaatggac tgggacgcgt tgcctggcgg ttgccggagc agagttcaac
    48901 cattctggtg ctgaataaag acgggcaaat tcagtgggca aaggacggca gcctgacgcc
    48961 ggaagaggta gatcatgtca ttgcgctggc gcagaaactg atcaatgaat aaaaaattca
    49021 aaggattgag ggtgttagtg agatgaagct ggttgaacac tacatcatgc gtggtacgcg
    49081 ccgtctggtg ctgattatcg tcggttttct gatctttatt ttcgccagct actccgcaca
    49141 gcgttatctg accgaagcgg caaatgggac cttagctctt gatgttgtgc tggatatcgt
    49201 tttctacaaa gtgctgattg cactggagat gttgttacct gttggtctgt atgtgtcagt
    49261 tggcgtgacg ctagggcaga tgtataccga ctcggaaatt accgctatct ctgcggcggg
    49321 gggcaatccg ggacgcttgt acaaagccgt tctttatctg gcgataccgc taagtatttt
    49381 tgtcaccctt ctgtcgatgt atggtcggcc gtgggcttat gcgcagattt atcaactgga
    49441 gcaacagtcg cagtcggagc tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa
    49501 cgataacgga cgaatgatcc tttcgcagac ggttgatcag gataataatc gcctgactga
    49561 cgcgctgatt tatacttcta ctgccaatcg aacccgcatt ttccgcgccc gttcggttga
    49621 tgtggttgac ccatcacctg agaaaccgac cgttatgttg cataacggga ccgcctatct
    49681 tctcgatcat caggggcgtg acgacaacga acagatctac cgtaatctgc aattacatct
    49741 gaatccgctg gatcaaagcc ctaacgtcaa acgcaaagca aaatcggtca cggagctggc
    49801 gcgctcagcc tttcctgccg atcatgccga actgcaatgg cgacaaagcc gtggcctgac
    49861 agcattgttg atggcgctgc tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg
    49921 attttcaacg ttattgccac tgacattgct gtttgttgcc attttttatg gcggcgacgt
    49981 ctgccgtacg ctggtggcta acggtgcgat tcccctcatt cctggtttgt ggttagtacc
    50041 cggactcatg ctaatgggcc tgctgatgct ggtcgcacgc gacttctctt tgctgcagaa
    50101 attttcccga tgaatgtttt cagtcgctat ttaatccgtc atctctttct cggttttgcc
    50161 gccgccgcag ggctattgct gccgcttttt accaccttca acctgattaa cgaactggat
    50221 gatgtcagcc cgggcggtta tcgctggact caggcggtgc tggtggtgct gatgacctta
    50281 ccgcgtacac tggtcgaact ttcgccgttt atcgcattat tgggagggat tgttggcctg
    50341 gggcagttat cgaaaaacag tgagcttacc gccattcgca gcactgggtt ttctatcttc
    50401 cgtattgcac tggtggcgct ggttgccggg atattgtgga ctgtttcgtt aggcgcgata
    50461 gatgagtggg tggcgtcgcc attgcagcaa caggcgctgc aaatcaaatc gactgccacc
    50521 gcgttggggg aggacgatga cattaccggc aatatgcttt gggccaggcg cggtaatgaa
    50581 tttgtgacgg tgaaatcgct gaacgagcag ggccagcctg tgggcgtgga gatatttcat
    50641 tatcgcgacg atctctcgct cgaatcctac atttttgcac gcagtgcctc cattgaagac
    50701 gacaaaacgt ggatcctgca tggtgtgaat cataaaaaat ggttgaatgg caaagaaacg
    50761 ctggaaacat cagataatct tgcctggcaa tcggccttca ccagtatgga tcttgaagag
    50821 ttatcgatgc cggggaatac tttttctgtc cgtcagctta atcattacat ccattatttg
    50881 caggaaaccg gacaacccag cagcgaatac cgccttgcac tgtgggaaaa actggggcaa
    50941 ccgatcctga ccctggcgat gattttgctg gctgtgccgt tcacctttag cgccccgcgc
    51001 tcgccaggga tgggtagccg tctcgctgta ggcgtcatcg ttggcttact cacctggatc
    51061 agctatcaaa tcatggtcaa tctgggattg ttatttgcgt tgagcgcacc tgttaccgcg
    51121 ctcggtttac cggtagcgtt tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga
    51181 caacattaaa cccgccgtgg cgcaacacca cagcgggctc acattactgc ttcagagtag
    51241 caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcgttt acgctacagc
    51301 gcagcagagt gatgcctgca ggcgcggcag gtggcaggat aagattcaca taaacgccca
    51361 gcgaaataag ctcgcgccag atgcgtaaac cttcctcttt cgaaccgatt attactggca
    51421 ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg tcatacagat
    51481 ggttggcgtt atccatcagt ttttgccgca gctctggttg tgtggcaatg gttcttaacg
    51541 acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata tacgggcggc
    51601 tgccgtagcg cagcacttcc atcgcttccg aaccgacagc aaaaccgcca atcgaggcca
    51661 gacttttact gaaagtacca acgataatat ccacatcatc ttcaacgcca acggcttcag
    51721 ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg cgcttcatcg acaatcaaat
    51781 aaccaccgag acgacgtttg atatcaacaa tttccgctaa cggcgcaacg tcaccgagca
    51841 tgctgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg aggcgaacca
    51901 tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttct gcgccaccga
    51961 gagaacaggc gtcataaata ctggcgtggc tgtcggcatc cagtaacacc acggcgttat
    52021 ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa aaaacgatag
    52081 cggtagggcg attgaaaaac tcggcaatct ctttctctaa cgccaggtgc ggcgcataac
    52141 tgccgtttgc catacgagaa ccggtggtgc cggtgccttg ggccgctaac gctgcctgac
    52201 cttcggcgat ggcatcatga ttaaaggtta aaccgagata attattggtc ccggccagaa
    52261 tcactttttt attgccgatc cgaccttcgg tggcggaata cacttcatca atacaggtgc
    52321 caaacggtgt taacccgttg tcactaaatt gcagtcgctc gccagcaagg cgcgcatatt
    52381 tatcgtatag ccccattatt tattctccag ccatggaagt agagtttcca ttaattgcgc
    52441 aggggttttg acatccagca gaatattaat aggaatggaa atatcgaatc tgtcttccag
    52501 catcattaag agatccatca ctttgatcga ctccaggcca agatcgttga cgagatcgct
    52561 gtcgggctta atttccacac cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc
    52621 cattactatt tcacgattta ccatagaaac gttaacacac ctttaaacaa gacaatcaaa
    52681 atagcccgtt gcgtaatgcg tgttccaggc taatcccggg aaaccaatta atatcttcag
    52741 aaatacggtt attacttgcc gaccagtcgg catgggttaa ttcacggatt ttcgaacgag
    52801 tcagcatcgg ttctttaccc gccagacggc tcaacgcggt actgatatcc gcaaggcagg
    52861 tgagtagcgg cagaggaata ccgaccatcc gcacggaacc acagcggaca tcggcaacaa
    52921 gctgttggac acgttgccag tcatagcctc cggcgacgcc atcgcataat tcataggttt
    52981 gcgtctgtac agtttcggcg cttaaccact gacccactgc ttgcgcaaaa tcggtgacgt
    53041 gcaggaaaga gagctgtgtc tctggcgtgc caagtcgtgg cagcagaccg cgcagcatcc
    53101 agtcaaacag cggttttaac tctttatcgc cgggaccata cacggctgtc gggcgaaaaa
    53161 cgcccaacgt aattgcgtca gccattgcag tcagctgttg ttcggcgacg tgtttggaat
    53221 ttgcgtacca ggagagttcg ggatggcgcg ccgccagcga agagataaac agaaatcgtt
    53281 ggcaaaagcc gctctctttt gccgcttgca tcaggcgcag gctgccgtca acgttgcagc
    53341 gggtaaaaat ctcttctttg tgcccgcgca cttgcccggc gcaatggacc accgcgctgg
    53401 ctccggtaac cagttcgcta agcgaatgtg tatcttccag cgaaccgcgc acccaagtga
    53461 gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc gcgaacgtga aagccgcggg
    53521 cgagcaggtt atcaataata tatttaccga taaacccggt agcgcccgtc accgcgacag
    53581 tttgattcat gccagggact cctgcacatg aagactggca gcataagcct tctgataacg
    53641 ttttttcgct tccgcacggg caggcttgcc ggaggatgtt cggggaatac tgtggggcgg
    53701 caacagatcg atatccgcgg taacgccaaa ttcgctttgg atccgtgctg ccagcgcgtg
    53761 gataagctgc ccgcgacgtt cttcatcgct gatccgacac tggatctgca aaatgatttt
    53821 ttcctgggcg gtaacaaaag caatcgcatc gccagaatga atttccggtt cctgttccgc
    53881 aatatattca atatcctgcg gccagatatt acggccacga ataataatca gatctttaat
    53941 gcgtccggtg acatacagat aaccgtccag cagataaccg aggtcgccgg tgtctaacca
    54001 gcccgtcgcg gcaatctcgt cttgtgaaat ctggtcgcca aagtaaccgc tcatcaggct
    54061 agggccggag atgcaaatat ggcctaccac acgttccgcg acaggtatac ctgcttcatt
    54121 gcggatttca atgccatgtt ccggcaacgc tttaccgcag ttgacgaaag tcgatacggc
    54181 gcgtgtttca gcacccggcg cgacggcttt accctggtat tcgaggatgt cgcgatccac
    54241 ttcgttaacc acaaccccgg aagcttcatc agagaagctg acagccagcg cattttctgc
    54301 cagtccgtag cacggcatga aagttttatc gtcaaagttg acctgacgga aacattcagc
    54361 gaattgatgg agttgttctg cggagatggg ttctgcacca ataccagcga cgcgccagca
    54421 ggaaagatcc agttcagcga gatctttttc attcacgcgg cgctggcaca attcatagcc
    54481 aaacggcggc gcaacggaaa cggtgccgcg atttttactg atcaatttaa gccattgcag
    54541 aggacgcatg gcaaaatcct gagtgcgcaa ataatctact gaaagctgcg tggcgacggg
    54601 ggtcaggaga aagccgacca gtcccatatc atggtagaaa ggcagccagg agacgcagcg
    54661 gtcgccaggg cgtaatttaa tcccatcgtg gcttatagca cgtagattag ccatcacttc
    54721 gcggtgagta atgataacgc cacggggaaa acgggtgctg ccggaggtgt actggaggta
    54781 ggcgatatcg tttggaactg gacgttgaag cgcaacatcg gcttccggta atgccttaaa
    54841 ccaggcgtgg cttaaaacat gtaattcggg attgttatgc gtcgcggcat tgaccagtgg
    54901 caaccactca tcaccagtga taatggctgc gggctggcaa cttgccagta acccctgcag
    54961 tttggcgctc caggaatccc gctgaccgac gcccattgga atcgccaacg ggacggcgac
    55021 taagccggca tactggcagg caaaaaaagc ctctacaaac ccgctactcg tttcggcaat
    55081 cagtgccacg cgatcgcctt ttttcaggtt cagcgataac aaccgcttcg caccagcttc
    55141 ggcacgcgct tttaacgtct gatattccag ttgatcttca agttggcaac gtctgtcata
    55201 aaaattcatt ccagcgctac tcagagcggc atagtccaaa gcatcaacca gcgttggaaa
    55261 atcggcatag cgcataggta gggaatgcgt aaagatttta ttagacatat acaccacgaa
    55321 aaaattggat atcaaaaata aaatgacaat aatgcggtgg attatatagg cataaaagat
    55381 aaatgagata gtattttcat cggtgcgata ttgaacattg atttaaataa taggttttgt
    55441 tttgttgttt ttattgtgaa tgatatttga tttatgaatg tattaagaat gaaccttgtc
    55501 tgaccgcatt aaatcgctaa aaggcattgt ggcgcttctt cgttggctgt gatcttatcg
    55561 taatcattaa taaataatat ttattcttat tttgtattca tggtgttgat tattaagatg
    55621 ccgttcgttg ctgttgcaac ctgtttttca tcgttaatta tttgttatcc acagagatat
    55681 ggaatgcgat ctgctgatga ttaaaattga aaaagtaatt aataaaaatg accttaaggc
    55741 atttatcgct tttccgtcat cactttatcc cgacgatcca aactggatac cccctttatt
    55801 cattgaacgc aacgagcatt tgtctgcgaa aaatccgggg acggaccata tcatctggca
    55861 ggcgtgggtg gcaaaaaaag cggggcagat agttgggcgt attaccgcgc aaatagatac
    55921 cttgcaccgc gagcgttacg gcaaagatac cggtcatttc ggcatgattg acgccattga
    55981 tgatccgcag gtttttgccg cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg
    56041 tgcaagtaag atcagcggtc ctttcagcct gaatatcaat caggaaagcg gattactgat
    56101 tgaaggtttc gatacaccac cctgtgcgat gatgccgcac ggtaaaccgt ggtatgccgc
    56161 gcatattgaa caattgggtt atcacaaagg cattgattta ctggcgtggt ggatgcagcg
    56221 aaccgatctc actttctctc cggcgctaaa aaaactgatg gatcaggtgc gtaagaaggt
    56281 gaccattcgc tgcatcaatc gccagcggtt tgccgaagag atgcagatcc tgcgtgagat
    56341 tttcaattct ggctggcagc acaactgggg atttgtgccg tttaccgaac atgaattcgc
    56401 gaccatgggc gaccaactta aatatctggt gccggacgat atgatctaca tcgctgagat
    56461 tgattctgca ccctgcgcgt ttattgtcgg cttgccaaac atcaacgagg cgattgccga
    56521 tctgaacgga tcgctctttc ccttcggctg ggcaaaattg ctgtggcgtt taaaagtcag
    56581 cggtgtgcga actgcgcgag taccgctgat gggcgtgcgg gacgagtatc agttcagccg
    56641 catcggcccg gtgattgcgt tgttattgat tgaagcctta cgcgatccgt ttgcccgccg
    56701 gaagatcgat gcgctggaga tgtcatggat cctcgaaacc aatacaggca tgaataacat
    56761 gctggaacgc attggggcgg agccgtacaa gcgttaccga ttgtatgaaa agcagatttg
    56821 atggcagtca cccctgcttc atctccagcg gcaccgccga gcgaatcaaa tggtggcctt
    56881 ccagctcgat atcgtggaga ttctttttca cggtccgcac atgatcgcgt gctgcgcgct
    56941 gggcgacgtg cggcagccgc tgcaacaccg cgttgtagat ccgcgcatgc tggcgatcga
    57001 tctgcttttt ttgctgtggt cgatggtaga gattatttac gctggcaaac accgaattaa
    57061 acatcaggtc ggtcaatgat tgcagcgtaa actccagcac ctgattgtga gaagcctgac
    57121 aaatggcgag atggaaagcg tgatccaact gcgcatgttc gatcagcgaa atctctttgt
    57181 tgttctcact ggcggcgagc attttttcat aacagcgggt tatcacaaca aaatcagcct
    57241 gggttcccag cgttgccgcc agccttgccg attcgccctc cagtaatgcg cgaacgtcga
    57301 gcagatcgta cagcgttcgc ggctgcgtac tgaacagatg gatcagcggg ctggtgtcct
    57361 gcacccgatt aagccgtgcg acacgagaat cgcgaccctg cgccgtttca ataatcccgc
    57421 gcccgcgcag cacggtcagc ccttcacgca gtgcggagcg tgagaagccg agcttttcac
    57481 acagtcgacg ttccgaggga agcggctgac cgaccttcag tacgccgtcg ataattaacc
    57541 gttcgatact ctctgcaacc acttcgcaaa tagggcgacg ttcatctttc attcccggac
    57601 ctcgtgcaca ggtaggacca atttttctgt tcttaactcg caaaacacgc acatcacgta
    57661 agtgattatg ttacatcaat ttaacattaa gttaaccaag acaaggtcac agagctggaa
    57721 aaaaaatggt ctgaccggta gctaaagaga tagacgaaaa cgaaaagccc gcttaataac
    57781 tgttcacaga agcagcgcga aaaaatcagc tgccacacaa cacaacaaag cgaagcctac
    57841 tcatgagcat cttgtacgaa gagcgtcttg atggcgcttt acccgatgtc gaccgcacat
    57901 cggtactgat ggcactgcgt gagcatgtcc ctggacttga gatcctgcat accgatgagg
    57961 agatcattcc ttacgagtgt gacgggttga gcgcgtatcg cacgcgtcca ttactggttg
    58021 ttctgcctaa gcagatggaa caggtgacgg cgattctggc tgtctgccac cgcctgcgtg
    58081 taccggtggt gacccgtggt gcaggcaccg ggctttctgg tggcgcgctg ccgctggaaa
    58141 aaggtgtgtt gttggtgatg gcgcgcttta aagagatcct cgacattaac cccgttggtc
    58201 gccgtgcgcg cgtgcagccg ggcgtgcgta acctggcgat ctcccaggcc gttgcgccgc
    58261 ataatctcta ttacgcgccg gatccgtcct cacaaatcgc ctgttcgatt ggcggcaatg
    58321 tggcggaaaa tgccggtggc gttcactgcc tgaaatatgg tctaaccgta cataacctgc
    58381 tgaaaattga agtgcaaacg ctggatggcg aggcgctgac gctgggatcg gacgcgctgg
    58441 attcgcctgg ttttgacctg ctggcgctgt ttaccggatc ggaaggtatg ctcggcgtga
    58501 ccaccgaagt gacgttaaaa ctgctgccga agccgcccgt ggcgcgggtg ctgttagcca
    58561 gctttgactc ggtagaaaaa gccggacttg cggttggtga catcatcgcc aatggcatta
    58621 tccccggcgg gctggagatg atggataacc tgtcgatccg cgctgcggaa gattttatac
    58681 atgccggtta tcccgtcgat gccgaagcga ttttattgtg cgagctggac ggcgtggagt
    58741 ctgacgtaca ggaagactgc gagcgggtta acgacatctt gttgaaagcg ggcgcgactg
    58801 acgtccgtct ggcacaggac gaagcagagc gcgtacgttt ctgggccggc cgcaagaacg
    58861 cattcccggc agtggggcgc atttccccgg attactactg tatggatggc accatcccac
    58921 gccgcgcgct gcctggcgta ctggaaggca ttgcccgttt atcgcagcaa tatgatttac
    58981 gcgttgccaa cgtctttcat gccggagacg gcaacatgca cccgttaatc cttttcgatg
    59041 ccaacgaacc cggtgaattt gcccgcgcgg aagagctggg cgggaagatc ctcgaactct
    59101 gcgttgaagt tggcggcagc atcagtgggg aacatggcat tgggcgcgaa aaaatcaatc
    59161 aaatgtgcgc ccagttcaac agcgatgaaa tcacgacctt ccatgcggtc aaggcggcgt
    59221 ttgaccccga tggtttgctg aaccctggga aaaacattcc cacgctacac cgctgtgctg
    59281 aatttggtgc catgcatgtg catcacggtc atttaccttt ccctgaactg gagcgtttct
    59341 gatgctacgc gagtgtgatt acagccaggc gctgctggag caggtgaatc aggcgattag
    59401 cgataaaacg ccgctggtga ttcagggcag caatagcaaa gcctttttag gtcgtcctgt
    59461 caccgggcaa acgctggatg tgcgttgtca tcgcggcatt gttaattacg acccgaccga
    59521 gctggtgata accgctcgtg ccggaacgcc gctggtggcg attgaagcgg cgctggagag
    59581 cgccgggcaa atgctcccct gcgagccgcc acattatggt gaagaagcta cctggggcgg
    59641 gatgatcgcc tgcgggctgg cggggccgcg tcgcccgtgg agtggttcgg tccgcgattt
    59701 tgtcctcggc acgcgcatca ttaccggcac tggaaaacat ctgcgttttg gtggtgaagt
    59761 gatgaaaaac gttgccggat acgatctctc acggttaatg gccggaagct acggttgtct
    59821 gggtgtgctc actgaaatct cgatgaaagt gttaccgcga ccacgcgcca ccctgagtct
    59881 gcgtcgggaa atcagcctgc aagaagccat gaatgaaatc gcccagtggc aactccagcc
    59941 attacccatt agtggcttat gttacttcga caatgcgttg tggatccgcc ttgagggcgg
    60001 cgaaggatcg gtaaaagcag cgcgtgaact gctggggggc gaagaggttg cgggtcagtt
    60061 ctggcagcaa ttgcgtgaac aacaactgcc gttcttctcg ttaccaggta ccttatggcg
    60121 catttcctta cccagcgatg cgccgatgat ggatttaccc ggcgagcaac tgatcgactg
    60181 gggcggggcg ttacgctggc tgaaatcgac agccgatgac aatcaaatcc atcgcattgc
    60241 ccgcaacgct ggcggtcatg cgacccgctt tagtgccgga gatggtggtt ttgccccgct
    60301 accggctcct ttattccgct atcaccagca gcttaaacag cagctcgacc cttgcggcgt
    60361 gtttaatccc ggtcgcatgt acgcggagct ttaaggagca ggctatgcaa acccaattaa
    60421 ccgaagagat gcggcagaac gcgcgggcgc tggaagccga cagcatcctg cgcgcctgtg
    60481 ttcactgcgg attttgtacc gcaacctgcc caacctatca gctcctgggc gatgaactgg
    60541 acgggccgcg cgggcgcatc tatctgatta aacaggtgct ggaaggcaac gacgtcacgc
    60601 ttaaaacaca ggagcatctc gatcgctgtc tcacttgccg caattgtgaa accacctgtc
    60661 cttctggtgt gcgctatcac aatttgctgg atatcgggcg tgatattgtc gagcagaaag
    60721 tgaaacgccc actgccggag cgaatgctgc gcgaaggatt gcgccaggta gtgccgcgtc
    60781 cggcggtctt ccgtgcgctg acgcaggtag ggctggtgct gcgaccgttt ttaccggaac
    60841 aggtcagagc aaaactgcct gctgaaacgg tgaaagctaa accgcgtcca ccgctgcgcc
    60901 ataagcgtcg ggttttaatg ctggaaggct gcgcccagcc tacgctttcg cccaacacca
    60961 acgcggcaac tgcgcgagtg ctggatcgtc tggggatcag cgtcatgcca gctaacgaag
    61021 caggctgttg tggcgcggtg gactatcatc ttaatgcgca agagaaaggg ttagcgcggg
    61081 cgcgcaataa tattgatgcc tggtggcccg cgattgaagc aggtgccgag gctattttgc
    61141 agactgccag cggctgcggc gcgtttgtca aagagtatgg gcagatgctg aaaaacgatg
    61201 cgttgtatgc cgataaagcg cgtcaggtca gtgagctggc ggtcgattta gtcgaacttc
    61261 tgcgcgagga accgctggaa aaactggcaa ttcgcggcga taaaaaactg gccttccact
    61321 gcccgtgtac cctacaacat gcgcaaaagc tgaacggcga agtggaaaaa gtgttgcttc
    61381 gtcttggatt taccttaacg gacgttcccg acagccatct gtgctgcggt tcagcgggaa
    61441 catatgcgtt aacgcatccc gatctggcgc gccagctgcg ggataacaaa atgaatgcgc
    61501 tggaaagcgg caaaccggaa atgatcgtca ccgccaacat tggttgccag acgcatctgg
    61561 cgagcgccgg tcgtacctct gtgcgtcact ggattgaaat tgtagaacaa gcccttgaaa
    61621 aggaataaca aaatgaaaac taaagtcatt cttagccagc aaatggcgag tgcaattatt
    61681 gccgcaggtc aggaagaggc gcagaaaaac aactggtctg tttccattgc tgttgccgat
    61741 gacggcggtc atctgctggc gttaagtcgc atggacgatt gcgcgccgat tgcggcttac
    61801 atctcccagg agaaagcgcg taccgccgcg ctggggcgtc gtgaaactaa ggggtatgaa
    61861 gagatggtga acaacggacg taccgcgttc gtgactgcgc cgttattaac gtcgctagaa
    61921 ggtggcgtac cggttgttgt ggatgggcaa attattggtg ccgtgggcgt ttctggttta
    61981 accggagcac aggatgcgca ggtcgcgaaa gcggcagcag cggtgttggc gaaataagcg
    62041 aaaacgagga gagaaacaat gagtcaaacc ataacccagg gccgtttacg cattaacgcc
    62101 aattttaaac gttttgtgga tgaagaagtt ttaccgggaa cagggttgga cgctgcggcg
    62161 ttctggcgca attttgatga gatcgttcac gatctggcac cagaaaatcg tcagttgctg
    62221 gcagaacgcg atcgcattca ggcggcgctt gatgagtggc atcgcagcaa tccggggccg
    62281 gtaaaagata aagcggccta taaatctttc ctgcgtgaac tgggctacct ggtgccgcaa
    62341 ccggagcgcg taacggtgga aaccacgggc attgacagcg aaatcaccag tcaggcgggg
    62401 ccacagctgg tggttccggc aatgaacgcc cgctacgcgc tgaacgcggc gaacgctcgc
    62461 tggggctcac tgtacgatgc gttatacggc agcgacatca tcccgcagga aggggcgatg
    62521 gtcagcggct acgatccgca gcgtggtgag caggttatcg cctgggttcg gcgtttcctc
    62581 gatgaatcac taccgctgga aaacggcagc tatcaggatg tggtggcgtt taaggtggtc
    62641 gataaacaat tacgcatcca gttgaaaaat ggtaaagaaa ccacgttacg taccccggcg
    62701 cagtttgtcg gttaccgtgg cgatgccgct gcgccgacct gcattttgct gaaaaataac
    62761 ggtctgcata ttgaactgca aattgatgcc aacgggcgga ttggcaaaga cgatccggcg
    62821 catatcaacg atgttatcgt cgaagcggcc atcagtacca ttctcgactg cgaagattcg
    62881 gtcgcggcgg ttgatgcgga agataaaatc ctgctgtacc gcaacctgct gggcctgatg
    62941 caggggactc tgcaagagaa aatggaaaaa aacggtcggc aaatcgtgcg taaactgaat
    63001 gacgatcgtc attacaccgc cgccgatggc tctgaaattt ctctgcacgg acgctcgctg
    63061 ctgtttatcc gcaacgtggg tcatttgatg accattcctg tgatttggga cagcgaaggc
    63121 aatgaaatcc cggaaggcat tcttgatggc gtcatgactg gcgcgattgc cctctatgat
    63181 ttaaaagtgc agaaaaactc gcgcactggc agcgtctata ttgtgaaacc gaaaatgcac
    63241 ggtccgcagg aagtggcgtt cgccaacaaa ctgtttaccc gcattgagac aatgctcggt
    63301 atggcaccga ataccctgaa aatgggcatt atggatgaag aacgtcggac ctcgctgaac
    63361 ttgcgtagct gtatcgctca ggcgcgtaac cgcgtggcgt tcatcaatac cggtttcctc
    63421 gaccgtaccg gcgatgaaat gcattcggtg atggaagctg gcccgatgct gcgtaaaaac
    63481 cagatgaaat cgacgccgtg gatcaaagcc tacgaacgta ataacgtgct ttccggtctg
    63541 ttctgtgggc tgcgcggtaa agcgcaaatc ggtaaaggga tgtgggcaat gccggacctg
    63601 atggcagaca tgtacagcca gaagggcgac caactgcgtg ccggggcaaa cacagcctgg
    63661 gttccgtcac caaccgctgc tacgctccat gcgctgcact accaccaaac caacgtacag
    63721 agcgtgcagg ccaacattgc ccagagcgag ttcaatgctg aatttgaacc gctgctggac
    63781 gatctgctga ctattccggt ggctgaaaac gctaactggt cggttgaaga gatccaacaa
    63841 gagctggata acaacgtgca ggggattctg gggtacgtgg tgcgttgggt ggagcagggg
    63901 attggttgtt caaaagtgcc ggatattcac aacgtggcgc tgatggaaga ccgcgcaacg
    63961 ctgcgtatct ccagccagca tatcgccaac tggttacgtc acagtattct gaccaaagaa
    64021 caggtgcagg cgtcgctgga gaatatggcg aaagtggttg atcagcaaaa cgctggcgat
    64081 ccggcttatc gtccgatgtc ggggaatttc gctaactcgt gtgcttttaa agctgccagc
    64141 gatttaatct tcctcggcgt gaaacagcca aacggctata ccgaaccgtt attacacgcc
    64201 tggcgtttac gcgaaaaaga aagtcattaa tagcgtcaaa aaagccccgg caagttttat
    64261 tgtcggggct atgatagtaa ctgcggtagt acatttatcc cggaaataac cggagcgatt
    64321 atcagggttt aacaatatta aatacccgta gcgcattact agcgggccta aaacaacaat
    64381 atggacatct taacgatatt ctgcgcctgc gtgtgcggaa cagaaaccct tacccgatat
    64441 acttgttatg ccagaggtag caattgcgtt agcagcgtac tgttgctgta aagtataacg
    64501 cctatatttt acagttcagt cctcttttgc ttattacaac cttaagtata tcaagcatat
    64561 aaagataata agagactgaa aaatatggtt accaggaccc aaatgtatat gccgatggga
    64621 ggactggggc tatccgctct ggtcgccctg atcccgataa tattcttctt cgttgcactc
    64681 gcggtattac gtctgaaagg acatgtcgcc ggagcaataa cccttatatt atctattctg
    64741 attgcgatat tcgcctttaa aatgccgatt gatatggcat ttgctgctgc gggctatggc
    64801 tttatttatg gattatggcc tattgcgtgg attattgttg cggcggtgtt cctctataaa
    64861 ttaaccgttg ccagcggtca gttcgatatt atccgcagct cggttatctc catcactgac
    64921 gatcagcgtt tgcaggtgtt actgattggt ttctcctttg gcgcgctgct ggaaggggcg
    64981 gctggctttg gtgcgccggt ggcgattacc ggtgcgctgc tggtgggcct gggcttcaaa
    65041 ccgttatacg cggcggggct gtgtctgatt gccaacactg cgccggtggc gtttggtgcg
    65101 ttgggcgtgc cgattctggt cgccggtcag gtaacgggaa tcgatccgtt ccacattggc
    65161 gcaatggcgg gacgtcagtt accgttcctg tcggttcttg tgccgttctg gctggtagca
    65221 atgatggacg gctggaaagg ggtgaaagag acgtggccag cggcgctggt tgctggggga
    65281 agcttcgctg tcacgcagtt ctttacctct aactatattg gtccggaact gccggatatt
    65341 acttcggcgc tggtgagtat cgtctcactc gctttattcc ttaaagtctg gcggccgaaa
    65401 aataccgaaa cggcaatcag catgggacaa tccgcaggtg cgatggtggt gaataaacca
    65461 tcttctggcg gtcccgtgcc ttcagaatat agtctggggc aaatcattcg tgcgtggtca
    65521 ccgtttttaa tcttaacggt gctggtcacc atctggacca tgaagccgtt taaagcgtta
    65581 tttgctccgg gcggcgcgtt ttattcactg gtgattaatt tccagatccc tcatttgcat
    65641 caacaagtgt tgaaagcggc acccattgtc gcccaaccaa cgccaatgga tgcggtgttt
    65701 aaattcgacc ctctctcggc tggcggcacc gctattttta ttgcggcgat tatctctatc
    65761 ttcatcctcg gcgtgggcat caagaaaggg attggcgtct ttgccgaaac gctaattagt
    65821 ctgaagtggc cgatattgtc gattggcatg gtgctggcgt tcgccttcgt caccaactat
    65881 tccggcatgt ccaccacgct ggcgctggta ctggcaggta caggcgtgat gttcccgttt
    65941 ttctcaccgt ttctcggctg gctgggcgta ttccttaccg gctcggacac ctcctctaac
    66001 gccctgtttg gttcactgca atcgaccacg gcgcagcaaa tcaacgtctc tgacactctg
    66061 ctggtggcag caaataccag cggcggcgta actggcaaga tgatttcccc gcaatctatc
    66121 gccgtggcct gcgccgcgac gggcatggtg ggccgagaat ctgaactgtt ccgctacacc
    66181 gtgaagcaca gtctgatctt tgccagtgtg atcggcatta tcacgctgtt gcaggcgtat
    66241 gtgtttaccg ggatgttagt ttcgtaagtt aataaagccg gatgatcgtt aatatcatcc
    66301 ggcttcgttt acattactct gtcttattaa tggaaaatta tttcccgctt tattatccat
    66361 gtggcaataa tgtttcgcgt tcagataacc tgctaaataa ttaaaagcgt gagaacatct
    66421 gtttttttat cacgcaaaac aagtcagtat taattatgtt cgcttgatga gcaaacaccg
    66481 ttctatctgt tgcttttgca atcttaatcg ttaaaactac caacaaattt atatagttac
    66541 gttgtgaaaa gttgttaatt ttttgtttat tgaaatttcg taaaaataac cagccagaca
    66601 tcgaataggg gttattctta taaatttcac ctgtttttct atcaatgatg ccgttttctt
    66661 aagaatggag gaagttatta aagaagataa agcataaaac tatttccccg gttacgaatc
    66721 ttttaacttt gttttgtcac ttgcgttatt aatgaataag aaatttaaat ataagaaatc
    66781 gcttttagcg gctattttga gtgcaaccct gttagccggt tgtgatggcg gtggctccgg
    66841 atcttcctcc gatacgccgc ctgtagattc tggaacaggg tctttgccgg aagtgaaacc
    66901 tgatccaaca ccaaacccgg agccgacgcc tgagccaacg ccggacccag aacctacgcc
    66961 ggaaccgaca cctgatcctg agccagaacc ggagccagaa cctgttccta cgaaaacggg
    67021 ttatctgacc ctgggcggaa gccagcgggt aactggtgct acttgtaatg gtgaatccag
    67081 cgatggcttt acatttacac ctggtgagga cgttacttgc gtggcgggta acacgacaat
    67141 tgctaccttc gacacccagt cagaagctgc gcgtagcctg cgtgcggttg aaaaagtgtc
    67201 gtttagtctt gaggacgcgc aagaactggc agcttccgat gacaagaaaa gcaatgcggt
    67261 ttcgctggta acgtccagta acagctgtcc ggcgaataca gaacaggttt gcctgacgtt
    67321 ctcctcagtg atcgagagta aacgtttcga ctcgctgtat aagcaaatcg atctggcacc
    67381 ggaagagttc aaaaagctgg tcaatgaaga ggtggaaaac aatgccgcga ccgataaagc
    67441 gccatccact catacttcac cggttgtgcc cgtcaccacg ccgggaacaa aaccggatct
    67501 gaacgcttcc ttcgtgtcgg ctaacgcgga acagttttat cagtatcaac ccactgaaat
    67561 cattctctcc gaaggccgac tggtagatag catgggcaat ggtgtggttg gcgtaaatta
    67621 ctacaccagc tcaggccgtg gcgtaactgg cgaaaacggc aaattcaact tcagctgggg
    67681 cgaaaccatc tcctttggta tcgacacctt tgaactgggc tcagtgcgcg gcaataagtc
    67741 gaccattgcg ttgactgaac tgggtgacga agttcgcggc gcgaatattg atcagcttat
    67801 tcatcgttac tcccaggccg gaaaaaatga tgagcgtgaa gtgccggacg tagtgcgcaa
    67861 ggtctttgcc gagtatccca acgtaatcaa cgagattatc aatctctcgt tatccaatgg
    67921 cgaggcgttg agcgaaggtg atcaaacctt tgagcggaca aacgaatttc ttgagcagtt
    67981 tgaatccggg caggctaaag agattgatac ggcgatttgt gactcccttg ggggctgcaa
    68041 ctctcagcgt tggttctcgt tgacagcacg caatgttaac gaaggtcaga ttcagggcgt
    68101 tatcaacaag ctgtggggtg tggataaaga ttacaaatca gtgacgaaat tccacgtctt
    68161 ccatgactct accaacttct atggcagtac cggtaatgcg cgcggtcagg cagtggtgaa
    68221 tatctccaac gcggccttcc cgattctgat ggcgcgtaat gataaaaact actggctggc
    68281 gtttggcgaa aaacgcgcct gggataaaaa cgagctggcg tacattacgg aagcgccttc
    68341 tctcgttgag ccggaaaacg ttacgcgcga taccgccacc tttaacctgc cgtttatttc
    68401 gctggggcaa gtcggtgagg gcaaactgat ggttatcggt aacccgcact acaacagcat
    68461 tttgcgttgt ccgaacggtt acagctggga aggcggtgtt gataaaaacg gtcagtgtac
    68521 gcgtaacagt gattctaatg atatgaagca ctttatgcag aacgtgttgc gctatctgtc
    68581 cgacgataaa tggacgccgg acgcgaaagc cagcatgacc gtaggcacca acctggatac
    68641 tgtctatttc aaacgtcatg gtcaggttac aggaaacagc gctgagttcg gctttcatcc
    68701 ggattttgcg ggtatctctg ttgagcattt aagtagctat ggcgatctcg acccgcagga
    68761 aatgccgctg ctgatcctca acggctttga gtatgtgact caggttggta acgatcctta
    68821 tgcaatcccg ctgcgagcag ataccagcaa accgaagctg actcagcagg atgtgaccga
    68881 tctgatcgcc tatctgaaca aaggtggatc ggtgctgatc atggaaaacg tgatgagcaa
    68941 tcttaaggaa gagagcgcgt ctggctttgt acgtctgctt gatgccgcag gtctgtcgat
    69001 ggcactgaac aagtcggtag taaataacga tccgcaaggg tatccgaacc gcgttcgtca
    69061 gcagcgcgca acgggcattt gggtctatga acgttatcct gccgtagatg gtgcgctgcc
    69121 gtacaccatc gatagtaaga caggggaagt taagtggaaa tatcaggtag aaaacaaacc
    69181 tgatgacaaa ccgaagctgg aagttgccag ctggctggaa gatgtagatg gcaaacagga
    69241 aacgcgttat gcctttattg atgaggccga tcataaaaca gaggattctc tgaaggctgc
    69301 gaaggcaaaa atctttgaga agtttcctgg attaaaggag tgtaaggacc caacttacca
    69361 ctacgaggtc aactgtctgg aatatcgtcc tggcacgggg gttccggtta ctggtggcat
    69421 gtatgttcca cagtatacgc aactaagcct taacgccgac acggcaaaag cgatggtgca
    69481 ggctgcggat ttaggcacca acattcagcg tctgtatcag catgagctct acttccggac
    69541 caatggtcgc aaaggtgagc gtctgagcag cgtcgatctg gaacgtctgt accagaacgt
    69601 gtcggtctgg ctgtggaata aaattgaata tcgctatgaa aacgacaagg atgacgagct
    69661 gggctttaaa acgttcaccg agttcctgaa ctgctacgcc aacgatgcct atactggcgg
    69721 cacgcagtgt tctgatgagc tgaaaaaatc gctggtcgat aacaacatga tctacggcga
    69781 gaagagcgtt aataaagcgg gcatgatgaa cccgagctat ccgctcaact atatggaaaa
    69841 accgctgacg cgcctgatgc tgggtcgttc ctggtgggat ctgaacatca aagttgatgt
    69901 cgagaagtat ccgggagcgg tatcggcaga aggtgagaag gttactgaaa ccatcagcct
    69961 gtactccaat ccgaccaaat ggtttgcggg taacatgcag tctactggcc tgtgggctcc
    70021 ggctcagaaa gaggtcacca ttgagtcttc tgcatcagtt cctgtgactg tcaccgtggc
    70081 gctggccgac gatctgaccg gacgcgagaa gcatgaagtc gcgctgaacc gtccgccaaa
    70141 agtgacgaaa acctatgatc tgaaagccaa tgataaggtg accttcaagg tgccttacgg
    70201 tggtctgatt tatatcaagg gcaacagccc aaagaatgag tcagccgaat tcacctttac
    70261 tggtgtggta aaagcgccgt tctataaaga cggcgaatgg aaaaacgctc tgaactcccc
    70321 tgcgccgctg ggcgagctgg aatcagacgc tttcgtttat accacgccga agaagaacct
    70381 tgaggccagc aatttcactg gtggtgtagc agaattcgct aaagatctgg atacctttgc
    70441 cagctcgatg aatgacttct acggtcgtaa tgatgaagac ggtaagcacc ggatgtttac
    70501 ctataaaaac ttgacggggc acaagcatcg tttcaccaac gatgtgcaga tctccatcgg
    70561 tgatgcgcac tcgggttatc cggtaatgaa cagcagcttc tcgacgaaca gcaccacgct
    70621 gccgacgacg ccgctgaacg actggttgat ctggcacgaa gtagggcaca acgccgccga
    70681 aacgccgctg actgttcctg gcgcaactga agtagcgaac aacgttctgg cgctgtacat
    70741 gcaggatcgc tatctcggca agatgaaccg tgtcgctgac gatattactg tcgcgccgga
    70801 atatctggag gagagcaacg gtcaggcatg ggcgcgcggc ggtgcgggcg accgtctgct
    70861 gatgtacgcg cagctgaagg agtgggcaga gaaaaacttt gatatcaaac agtggtatcc
    70921 agaaggtgac ctgcctgagt tctacagcga tcgtgaaggg atgaaaggct ggaacctgtt
    70981 ccagttgatg caccgtaaag cacgcggcga tgatgttggc aaaaccaagt ttggcgaaag
    71041 aaattactgt gccgaatcca acggtaacgc tgccgacaag ctgatgctgt gtgcctcctg
    71101 ggtcgctcag acggatcttt cggagttctt taagaaatgg aatccgggcg cgaatgctta
    71161 ccagttaccg ggagcgagcg agatgaactt cgagggcggt gtgagccagt cggcttacga
    71221 gacgctggcg gcgcttaatc tgccgaaacc gcagcaaggg ccggaaacca ttaatcaggt
    71281 taccgagcat aagatgtcag ctgagtaagc ctgtatgccg gataaggcgc tcgcgccgca
    71341 tccggcatga aataaggcgc atcgggcctg gaggaaagcc atatggcata cacccgcagg
    71401 cccgcatccg gcaagttaca acaaacaacc tttaaccatg ctttttgatg tttttcagca
    71461 atactccgcg gggatgccca tactggcaac cgtcggagga ttgatcatcg gcagtttttt
    71521 gaatgtggtg atttggcgtt accccatcat gctgcgccaa caaatggcgg agtttcacgg
    71581 tgaaatgccg agtacgcagt caaaaataaa cctggcgctg ccacgctcgc actgtccgca
    71641 ttgccagcag accatccgcg ttcgtgacaa tattccgctg ctctcatggt tgatgctcaa
    71701 agggcgctgc cgcgactgtc aggcgaaaat cagcaagcgt tatccgctgg tggagttatt
    71761 gacggcactc gcttttttgc tggcgagtct ggtgtggcca gaaagtggat gggggctggc
    71821 ggtgatgata ttatccgcct ggctgattgc cgcgagcgtc attgacctcg ataaccaatg
    71881 gctgcccgat gtttttactc agggcgtatt gtggaccggg ctgattgcgg catgggcgca
    71941 acagagtccg ttaacgctac aagacgcagt taccggcgtc ctggtggggt ttatcacttt
    72001 ttactccctg cgctggatag ccggaatagt tctgcgtaaa gaagcattag gcatgggcga
    72061 tgtattactt ttcgctgcgt taggtggctg ggtggggccg ttgtcgctac ccaatgttgc
    72121 tttaatcgca tcatgctgcg gcctgatata tgccgttatt acaaaaagag gatcaaccac
    72181 actgcctttt ggaccgtgtt taagtctggg cggtatagca acactttatc tacaggcatt
    72241 gttttaatga taaccacgtc attatcaaag tgacatttta actcttatta ataaccttag
    72301 agattattta ccatgtcgat aaaacaaatg ccagggaggg tattaatatc gctattgttg
    72361 agcgttacag gattattaag tggctgtgcc ggccataatg aaaatgccag tttactggcg
    72421 aaaaaacagg cgcaaaatat cagccaaaac ctgccgatta aatctgcggg atatacctta
    72481 gtgctggcgc aaagtagcgg cacgacggta aaaatgacca ttatcagcga agcgggtgct
    72541 cagaccacgc agacacctga tgccttttta accagctatc aacgacaaat gtgcgctgac
    72601 ccgacggtga aattaatgat taccgaggga attaattaca gcataacgat taatgataca
    72661 cgtacaggaa accagtatca gcggaaactg gatcgtacca cctgtggaat agtcaaagca
    72721 taacgtcggg tagatataaa ttggcgcggg ttgtttttcg tgacgcacga atttatctca
    72781 ttcagttgct gacaaaaatt cgtcacactc ttagccagag acaatctctt aatacagaca
    72841 aagagcatct gcgaaaaatg gtgcgcggga tgttctggct catgctgctt attatttctg
    72901 caaaaatggc gtattcactc tggcgctatt tctccttttc tgcggaatat acggcggttt
    72961 cctcatcggc gaataaaccg ctccgtgcgg atgcaaaacc gttcgataaa aatgacgtgc
    73021 aattagtcag ccaacaaaac tggtttggca aatatcaacc cgtcgccgca ccggtaaaac
    73081 aacccgaacc tgcgcctgtg gcagaaacgc gtcttaatgt ggtgctgcgc gggatcgcct
    73141 ttggtgccag acccggcgcg gtgattgaag aaggcggcaa acagcaggtc tatttacagg
    73201 gcgaacggct tggctctcac aacgcggtga ttgaggaaat taatcgtgac catgtgatgc
    73261 tgcgctatca ggggaaaatg gaacgtctga gtctggcaga agagaagcgt cccaccaaag
    73321 ccgtgaccag caaaaaagcc gtcagcgacg aagcaaagca agctgttgct gagcctgctg
    73381 ccagtgcgcc agttgagatc ccggctgccg tgcgtcaggc actggcgaaa gatccgcaga
    73441 aaatttttaa ctatatccag cttacgcctg tgcgtaagga agggattgtc ggttatgcag
    73501 tgaaaccggg ggcagatcgt tctctgttcg atgccagcgg ttttaaggaa ggcgatatcg
    73561 ccattgcgct aaatcagcag gatttcactg atccacgagc aatgattgct ctgatgcggc
    73621 agttaccttc aatggattcc attcaactta cggttttacg caagggtgcg cgctacgaca
    73681 tttccatcgc gctgcgctaa ccgcatttaa tccaggagaa tcattcatcg tgttttggcg
    73741 tgatatgacg ttgtcggtct ggcgtaagaa gacaactggc ctcaaaacaa aaaagcgttt
    73801 actggcgctg gtgctggcag cggcattatg cagttcaccg gtctgggcgg aagaagccac
    73861 tttcaccgct aattttaaag ataccgacct gaaatcgttc atcgaaaccg tcggcgctaa
    73921 ccttaataaa accatcatta tggggccggg cgttcagggg aaagtgagta ttcgcaccat
    73981 gaccccgctc aatgaacgcc agtattacca gctattcctt aacctgctgg aagcacaggg
    74041 gtatgccgtc gtaccgatgg aaaacgacgt gctgaaggtg gtgaaatcca gtgccgcgaa
    74101 agtcgagccg ctgccgctgg ttggtgaagg cagcgacaac tacgcgggcg atgaaatggt
    74161 caccaaagtc gtgccggtac gtaatgtgtc ggtacgcgag ctggcaccga ttctgcgcca
    74221 gatgatcgac agcgcaggct caggcaacgt tgttaattac gatccctcca acgtgattat
    74281 gcttaccgga cgcgcctcag ttgtggagcg cctgacggaa gtgatccagc gcgtggatca
    74341 tgcgggtaat cgcaccgaag aggtgatccc gctggataac gcctcggctt cggaaatcgc
    74401 ccgcgtgctg gaaagcctga cgaaaaacag cggcgagaac cagcctgcga cgctgaaatc
    74461 tcaaattgtc gccgacgaac gcaccaacag tgtgattgtc agtggtgacc cggccacgcg
    74521 ggacaaaatg cgccgcctga tccgtcggct ggactcagaa atggaacgca gcggcaacag
    74581 ccaggttttc tatctcaaat acagcaaagc cgaagatctg gtcgatgtac tgaagcaggt
    74641 cagcggcacg ctcacggcgg ctaaagaaga ggcggaaggc accgtcggta gcgggcgtga
    74701 ggttgtctcc atcgccgcca gcaaacacag taatgccctg attgttaccg cgccgcagga
    74761 catcatgcag tcgctgcaaa gcgtgattga acaactggat attcgccgtg ctcaggtgca
    74821 cgtcgaggcg ttgatcgtgg aagttgccga aggtagcaat atcaatttcg gcgtgcagtg
    74881 ggcgtcgaaa gatgccggat taatgcagtt tgctaacggt acgcagatcc ctattggcac
    74941 gctgggtgca gccatttctc aggcaaaacc gcagaaaggc tccacggtga tcagtgaaaa
    75001 cggcgctacc accattaacc cggatactaa cggcgatctc tccacgctcg ctcagcttct
    75061 ttctggcttt agcggtacgg cggttggcgt ggtgaaaggc gactggatgg cgctggtaca
    75121 ggcggtcaaa aacgactcca gctcgaacgt gctctccacg ccgagtatca ccacgctgga
    75181 caaccaggaa gccttcttca tggtgggcca ggacgttccg gtattaaccg gctctactgt
    75241 tggctctaat aacagcaatc ctttcaatac ggtagagagg aaaaaagtcg gcatcatgct
    75301 gaaagtcacg ccgcagatta acgaaggaaa cgcggtacag atggtgattg agcaggaagt
    75361 gtcgaaggtg gaaggacaga ccagtctcga cgtcgtgttt ggcgagcgca aactgaaaac
    75421 caccgtgctg gcaaacgatg gtgagctgat cgtgcttggc ggtctgatgg acgatcaggc
    75481 gggagaaagc gtggcgaaag tgccgctgct tggcgatatc ccattgattg gtaacctgtt
    75541 taaatcgacg gcggataaaa aagaaaaacg taacctgatg gtatttatcc gcccgaccat
    75601 tctgcgtgac ggtatggcgg cagacggcgt gtcgcagcgc aaatataact atatgcgcgc
    75661 cgaacagatc tatcgcgatg agcaaggctt gagcctgatg ccgcacaccg cgcagccggt
    75721 actgccagcg caaaatcagg ctctaccgcc agaagttcgc gcgttcctta atgccgggag
    75781 aacgcgttaa tggtgcctgt agcacaggaa accaccgcta acaccgtgcg tctgccctac
    75841 agtttcagcc gtcggtttag cctggtggca tggtgcgaag cgtcgctgga gatcctccat
    75901 gttcatccgt tgtcgctctc tgttttgcag gagctgcagc gggggctgaa cgcgtctttt
    75961 acgctgaggc aaatcgacga ggccgaattt gaacagcggc tgaatgcggt ctggcagcgg
    76021 gactcttccg aggcccgcca gctgatggaa gatctcggtt ctgctgagga cttttttact
    76081 ctcgcagaag aactaccgga aacggaagat ctgctggaaa gtgacgacga tgcgccaatc
    76141 atcaaactga tcaacgccat gttggcagag gcgattaaag aaggcgcttc ggatatccac
    76201 atcgagacgt ttgaaaagag tctggtgatc cgttttcgtg ttgacggcac actacatgaa
    76261 atgctgcgtc cggggcgcaa actggcctcg ctgctggtat cgcgtattaa ggtgatggca
    76321 cggctggata tcgccgaaaa acgcgtaccg caggatgggc gcattgcgct gctgctgggc
    76381 ggtcgggcga ttgacgtgcg tgtctccacc atgccttccg cctgggggga acgggtggtg
    76441 ctgcgactgc tggacaaaaa ccaggctcgc ctgacgctgg agcgtctggg tttaagtctc
    76501 gaactgactg cgcagttgcg ccagctgtta cacaaaccgc acggcatttt tctggtgacg
    76561 gggccgaccg gttccggcaa aagcaccacg ctgtacgctg gattgcagga gctgaacaac
    76621 cactcgcgta acattctcac ggttgaagac cctatcgaat acatgattga agggatcggt
    76681 cagacgcagg ttaacacccg cgtcggcatg accttcgccc gtggcctgcg cgcgattttg
    76741 cgtcaggacc cggatgtggt gatggtcggt gaaatccgcg ataccgaaac cgcagaaatc
    76801 gctgttcagg cttcactgac cggacacctg gtactttcca cgctgcatac caacacggcg
    76861 gtgggggcga tcacgcgttt gcaggatatg ggcgttgagc ctttcctgct ctcttccagt
    76921 ctgacgggcg tgatggcgca gcgactggtt cgcacgctgt gtcccgattg ccgccagtcc
    76981 gcgcctgcca ccgacgaaga aaaacgcctg ctggggatta ccgatgcgcg aaccgtcacg
    77041 ctgtaccatc cgcagggctg tcccgcctgt aatcacaaag gttttcgcgg acgtactgcc
    77101 atccatgagc tgatcgtggt ggacgccaca ttacgtgatt tgatccaccg tcaggccggg
    77161 gaactggagc tggaacgtta tgtccggcaa cactctgcgg gtatccgcag caacggcatt
    77221 gagaaagtgc tcgccggaga aacctctctc gatgaagttc tgcgggtaac catggaggcg
    77281 taatggcact gttttactat caggcgctgg agcgtaatgg tcgcaaaacc aaaggaatga
    77341 ttgaggcgga ttccgcgcgt catgcccgtc aattgttgcg tggtaaagag cttatccccg
    77401 tgcacattga agcccggctg aatgcttcga cagggggaat gttgcagcgt cggcggcatg
    77461 cacatcgtcg cgtggcggcg gcagatttgg cgctgttcac acgccaactg gcaacgctgg
    77521 tgcaggcagc aatgccgctg gaaacctgct tacaggcggt cagtgagcaa agtgaaaaac
    77581 tgcatgtaaa aagcctcgga atggcgctgc gcagccggat tcaggaaggt tataccctgt
    77641 cggacagcct gcgcgaacat ccccgcgtct ttgactccct gttttgttcg atggtcgctg
    77701 ccggagaaaa atccggacat ctcgacgtgg tgctcaatcg cctggcggat tacaccgaac
    77761 agcggcagca tctgaaatca cgcctgctgc aggccatgct ctacccgctg gtactgctgg
    77821 tggtggcaac gggcgtggtc actattttgc tgacggcagt agtgccgaaa attatcgaac
    77881 agtttgatca tctcggacac gcgctgcctg cctccacccg aatgctcatc gccatgagcg
    77941 acacgttaca gaccagcggc gtgtactggc tggcgggttt gctggggctt ctggtgctgg
    78001 ggcaacggct actcaaaaat cctgcgatgc gcctgcgctg ggataaaacc ttgctgcgtt
    78061 tgcccgtgac ggggcgtgtt gcgcgcggac tgaatacggc gcgtttttcc cgcacattaa
    78121 gcatcctcac cgccagcagt gttccgctgc tggaaggcat tcagaccgcc gccgccgtgt
    78181 cggcaaatcg ttatgtcgag caacaactgc tgctggcggc agatcgcgtc cgcgaaggaa
    78241 gcagcctgcg cgccgcgctg gcggagttgc gcctgttccc gccgatgatg ctgtacatga
    78301 tcgcctccgg cgaacagagc ggtgaactgg aaaccatgct tgaacaggcc gcggtcaacc
    78361 aggaacggga atttgatacc caggtggggc tggcgttagg gctgtttgag ccggcgctgg
    78421 tggtgatgat ggcgggcgtg gtgctgttta tcgtcatcgc catcctcgaa ccgatgctgc
    78481 aactgaacaa tatggttgga atgtaattta cggagttatc acatgaattc gttatcccgc
    78541 acacaaaaac cacgggcagg ttttaccctg ctggaagtga tggtggtgat tgttattctt
    78601 ggcgtcctag caagtctggt ggtgcctaac ctgttgggca acaaagagaa agccgatcgg
    78661 caaaaagcca tcagcgatat cgtggcgctg gagaatgcgc tggatatgta ccgactggat
    78721 aacgggcgtt atccgactac cgaacagggg ctggaggcgc tgatccagca accggccaat
    78781 atggcggatt cccgtaacta ccgtaccggt ggatacatta aacgactgcc aaaggatccg
    78841 tggggcaatg attatcagta tctcagcccg ggtgaaaaag ggctgtttga tgtttatacc
    78901 ctgggagcag atggtcagga aaacggggag ggcgctggcg cagatatcgg taactggaat
    78961 ttgcaggagt ttcagtaatc agtgcctgaa cgcggattca cacttctgga aatcatgctg
    79021 gtgattttcc ttatcggcct tgccagtgcg ggcgtggtac agacgtttgc gaccgcttca
    79081 gagccgcctg cgaaaaaagc ggcgcaggat tttctgactc gctttgcgca gtttaaggac
    79141 agggcagtga tcgaagggca aacactcggt gtgctaatcg acccgccagg ttaccagttt
    79201 atgcagcgtc gtcacgggca atggttgccc gtttctgcga cccggttatc ggcacaggtt
    79261 acggtgccga aacaggtgca gatgctgtta caacccggaa gtgatatctg gcagaaggaa
    79321 tatgcgctgg agctgcagcg tcgtcgcctg acgctgcacg atattgaact ggagctgcaa
    79381 aaagaggcga aaaagaagac accgcagatc cgtttttcgc cttttgaacc cgccacgccg
    79441 tttacactgc gtttttacgc gtcggcacaa aacgcgtgtt gggcggtaaa gctggcgcac
    79501 gatggcgtgt tatccctcaa tcaatgtgat gaggggatgc catgaagcgt ggatttacct
    79561 tgctggaagt aatgctcgcg ctggcgattt ttgcgttggc cgccatggcg gtgttacaga
    79621 tcgccagcgg cgcgctgagt aatcagcaaa tccttgaaga aaaaacggta gcgggctggg
    79681 tagctgaaaa ccagaccgca ctgctttacc tgatgacccg tgaacaacga gcggtcaggc
    79741 accagggcga gagtgatatg gcaggaagcc gctggtactg gcgaaccaca ccgctgagta
    79801 caggcaacgc gctgctccag gcagtggata ttgaagtcag ccgtcacgaa gacttttcgt
    79861 cggtgattca gtcacgacgc gcctggttta gcgccgtggg aggccaacag tgagaaggac
    79921 ttgcgctggt ttcacgttac tggaaatgct ggtggcaatt gccatttttg cctcactggc
    79981 actgatggcg ctgcaggtga caaacggcgt tacacgcgtg aatagcgccg tcgccggaca
    80041 cgatcaaaaa ctaaacctca tgcagcaaac gatgagtttt ttgaaccacg atctgacaca
    80101 aatgatgccg cgtccggtaa gaggcgatca gggccagcgt gaaccagcgt tactggcggg
    80161 cgctggcgtg ctggcgtctg agagtgaagg gatgcgtttt gtgcgcggcg gcgtggttaa
    80221 tccgttgatg cgtctgccgc gcagtaatct gctcaccgtc ggttaccgca ttcatggtgg
    80281 ttatctcgaa cggttagcct ggccactgac cgatgcggca ggcagcgtga agccaacaac
    80341 gcaaaaatta atcccggcgg attcgctccg tttgcagttc tacgacggca cgcgctggca
    80401 ggagagctgg tcatcagtgc aggcgattcc tgtggcagta cgcataaccc tgcattcgcc
    80461 gcaatggggc gaaattgaac gcatctggtt gttacgcggg ccgcaattat catgatcacc
    80521 tcaccaccaa aacgcggaat ggcactggtc gtggtgctgg tattgctggc ggttatgatg
    80581 ctggtgacca tcacgctttc cgggcggatg cagcaacaac ttgggcgaac gcgcagccag
    80641 caggagtacc agcaggcgct gtggtacagc gccagtgcgg aaagcctggc gttgagcgca
    80701 ctcagtctga gcctgaaaaa tgaaaagcgt gtgcatcagg cacagccgtg ggcttctggc
    80761 cctcgttttt tcccactgcc gcaggggcaa atcgccgtca ctctgcgtga cgcacaggcc
    80821 tgctttaacc tgaatgccct cgctcagccg acgacggcgt cgcgtccgct cgcggtacaa
    80881 caactgattg ccctgatctc gcgcctcgat gtgcctgctt atcgtgccga actgatagcc
    80941 gaaagcctgt gggagtttat tgacgaagac cgcagcgtgc agacgcgtct gggtcgtgaa
    81001 gacagcgagt atctcgcccg ttcggtgccg ttttacgccg ctaatcaacc gctggccgat
    81061 atcagtgaga tgcgcgtggt gcagggaatg gacgccggac tttatcaaaa actgaaaccg
    81121 ctggtctgtg cgctgccgat gacccgccag caaatcaaca tcaacaccct ggatgtcacg
    81181 caaagtgtga ttcttgaggc gctgtttgac ccgtggttaa gccctgtaca ggcgcgggcg
    81241 ttattacaac aacgtccggc gaagggctgg gaagatgtcg atcagtttct tgcacagccg
    81301 ctactcgcag acgtcgatga gcgtactaaa aaacagctaa aaaccgtcct gagcgtggac
    81361 agcaattact tctggctgcg ttcagatatc accgtgaatg agattgaact gacgatgaac
    81421 tcgttaattg tccgcatggg cccacaacac ttttctgttc tctggcatca gacaggagaa
    81481 agtgagtgag ttccatcctt gagatttttt tcccgctttg cgactccgac accgtccgct
    81541 ggcagcgccg tacacccgat gtggaacacg gtatctggcc tgacgttgct gacgaacgtc
    81601 tccagcaatg gctgcaaacc gatgcgattc gactctacat tcccggcgaa tggatcagcg
    81661 tctggcaggt tgaactgcct gatgtcgccc gcaagcagat accgactatt ctgcccgcct
    81721 tgctggaaga agagctgaat caggatatcg atgaactgca tttcgccccg ttgaaaatcg
    81781 accagcaact ggcaaccgta gcagtgattc accaacagca tatgcgtaac attgcgcaat
    81841 ggttgcaggc aaacggcatc acccgcgcta ccgtcgcgcc agactggatg tccattcctt
    81901 gtggctatat ggctggcgat gcgcaacggg ttatctgccg catcgatgaa tgccggggat
    81961 ggagcgccgg gcgggcgctg gctccggtca tgttccgcgc acagctcaat gagcaggatt
    82021 taccgttttc actaaccgtg gtcggcattg caccggaaga actgtctgca tgggctggtg
    82081 cagacgccga acgcctgaca gtgacggctc tgcccgccat taccacttat ggcgaaccgg
    82141 aagggaacct gctgactgga ccgtggcagc ctcgcgtcag ctaccgaaaa cagtgggcgc
    82201 gctggcgggt gatgattctg ccgatattgc tgattctggt tgcgctggca gtggaacggg
    82261 gcgtgacgtt atggagcgtc agcgaacagg tggcgcaaag ccgcacccag gcggagaaac
    82321 agttcttaac gctattcccg gagcagaagc ggattgtgaa tttacgctct caggtgacga
    82381 tggcgctgaa aaaatatcgc ccacaggccg acgatacccg gctgctcgca gaattgtcag
    82441 cgatcgccag taccctgaaa tcagcgtcac ttaccgacat cgaaatgcgt ggtttcacct
    82501 ttgatcaaaa acgccagacg cttcacctcc agctacgggc tgcgaacttt gccagcttcg
    82561 ataaactgcg tagcgcactg gcggcagatt atgttgtgca acaggacgcg ttacagaaag
    82621 agggtgatgc ggtttccggc ggcgtaacgt tgcggaggaa ataacatgtt acgcgataaa
    82681 tttattcact attttcagca atggcgtgaa cgccagttaa gccgtggcga acactggctg
    82741 gcacagcacc tggcggggcg ttcgccgcgt gaaaaaggca tgttactggc cgcggccgtg
    82801 ttcctgtttt gcgccgtata ttacgtcctc atctggcagc cgttgagcga gcggatagaa
    82861 caacaggaga cgatgttgca gcagctggtg gcgatgaacg cgcgactgaa gagcactgcg
    82921 ccggatatta ttgcggcgcg aaaatccggc acgacaacgc aagcacaggt atcgcgggtc
    82981 atcagtgaca gcgcttccgc gcactcggtg gtcatcaagc ggatagccga gcgtggggag
    83041 aatattcagg tctggataga acctgtggtg tttaatgacc ttctgaaatg gttaaacgca
    83101 ctggatgaaa aatatgcgct gcgggtgaca caaattgatg tcagtgctgc tgagaagcca
    83161 gggatggtaa atgtgctgcg actggagttt ggacgggggt aggtattgcc atttccttaa
    83221 ccccacctga taacccttag cccaatggtg agtgcggaac tgtcaaacca gctcaccggg
    83281 ctgagttccg atgtagcaag atttatctcc ctccagacca ccacggaatg ttcggtaaca
    83341 ctgaaggaac ctgtttttca ccatgtagcc ttcgttaatc gacacctttc taagactgta
    83401 aagatgctta agtgttttga cacattatga ttattaatag ttgtaataaa tcattgagta
    83461 acaattgata ggtcaaaaca tataggataa ttcttgtgtg atctgtattt tgtgttgctt
    83521 ggaaattagt aaaattcctg gagataatca gaaagggaat ttcaaataag cataataatt
    83581 tccagtgaaa ctattcgtcg attttaagaa atgtctgctc aggtatatct actgagggat
    83641 ggtgttggtt gtaacactgg ttaaaataaa tacacaaaat aatcaagatg tatattttaa
    83701 tcgacgaaaa taattacctt cgggattatt ggtgcgactt aaataacacc atttaaatgt
    83761 gatataaatc acaaatatgg ctgtaaagag ggggctgtag atataaataa gaagtacacg
    83821 agtgaatttt aaatagggaa atagtttctt ggtgaacaat ttattggtaa tcaatcgcgt
    83881 gcgttctggt ttgaaacgga ttgcaggtat tgttacgcat aaaatccata gggggtatta
    83941 taatcaagta gttaacagta ataataaaaa ataatttcct gagaaattaa atctgcattc
    84001 taaaacggcc agttttctga aattaccaga agaccgaatc cattcatgcc tgaagaatgg
    84061 attagaagaa acgagacgga aatagatcta tttatccctg cggaaataat ttctgctgaa
    84121 atttttttgc gactattaaa aaggtcaaac cgtctgagtg aattttatcc agttacaaat
    84181 aagcattacc tccagtgtat tggtagctgt taagccaagg gcggtagcgt acctgaagag
    84241 attaggatca catcatcaaa tggcaagaag tggatttgaa gtccagaaag tcaccgtaga
    84301 ggcattattt ctacgagaaa tacgaacacg ctttggtaag ttccgtctgg gatatttgtg
    84361 ggcgattctc gaaccctctg cgcatttgct gatactgttg ggcatttttg gttacattat
    84421 gcaccgcacg atgccagaca tctcattccc ggtgttttta cttaatggcc tgattccctt
    84481 ttttatcttt agcagtatca gcaatcgttc tgtaggcgct attgaagcga accaggggtt
    84541 gtttaattat cgaccagtaa aacccatcga tacgatcatt gcacgcgcac tgcttgagac
    84601 gctgatttac gatgctgttt atatattgct catgcttatc gtctggatgg caggtgaata
    84661 tttcgagata acaaactttt tacaactagt gctcacctgg agtttgttaa tcattctttc
    84721 atgtggcatc ggcttaatat tcatggtcgt tggtaaaacc ttccctgaaa tgcaaaaggt
    84781 cctgccgata ctgcttaagc cgctgtattt catctcctgc atcatgttcc ctctacattc
    84841 gattccgaag caatactggt catatctact ctggaaccca ttagtgcatg tcgtagagtt
    84901 aagccgcgag gcagttatgc ctggctatat cagcgaaggc gtgagtctga actaccttgc
    84961 aatgttcact ctggtcaccc tgttcatcgg tctggcgtta taccgaactc gtgaggaggc
    85021 aatgctgaca tcatgattaa gattgagaat ttgacgaagt catatcgtac gccaacgggg
    85081 cgacactatg tgtttaagga tttaaacatc gaaatccctt caggaaaaag cgttgccttt
    85141 attggtcgta atggtgcggg taaatcaacg ttactgagaa tgattggtgg cattgaccgc
    85201 cccgatagcg gaaagatcat caccaataaa acgatatcat ggccagtcgg cctcgccggt
    85261 ggattccagg gaagtttaac cggacgcgaa aacgtaaaat ttgttgcgcg gttatacgcg
    85321 aagcaagaag agctgaaaga gaaaattgag tttgtcgaag aatttgccga actcggtaag
    85381 tactttgata tgccgatcaa aacttactcc tcaggtatgc gatcgcgttt aggctttggt
    85441 ttaagcatgg catttaagtt tgattattat atcgtcgatg aagtaacagc tgtcggtgat
    85501 gccaggttta aagagaagtg cgctcaattg tttaaagaaa ggcataaaga gtctagcttt
    85561 ttgatggttt cacatagttt gaattcattg aaagagtttt gtgatgtggc gattgttttt
    85621 aaaaatagct atattattgg ttattatgag aatgttcaaa gtggtataga tgagtataaa
    85681 atgtatcaag ctttagatat tgaatgataa ttatattaaa taattaactc ttagttcagg
    85741 tatttatatg ttctgcttgt cattcgttgg tgttaataat aaaattaata tgacaaatag
    85801 catatgtatc ttatgaaatt gaaacgataa acttaatgac acaggtagaa tacaagtgac
    85861 ataagaaatt atgttgctgt ggctgatata agttcttttt gttcatttat ttttgtcgcc
    85921 aagcatcatg aatagaaatc cagatctcct aaaatcataa caaaattgtt aagataccgt
    85981 tctctgaata actagactag ctccctgaat ctctaggcaa tcaatatcat ttaaataact
    86041 gatagtagtg atactagtct tttagactag tagttggaga atagataatt ggtgtcttag
    86101 aagcagagaa acgcagacag cgtgccacac aggaaaatcg ctatttttca gcagagcctt
    86161 gaaccgaaaa tggcggtctt cctcgttgcc agacaacatg gtgtggcagg ctgccagtta
    86221 ttctttcagc gtaagcaata ctaagaggga ggcctgatta ctgttgcact aacagtgtca
    86281 ttgccggaga ttgtaatcac actctatata attatataaa ctctattgta tttagtgtat
    86341 gaggaggatg gacagtatac tttgaactag gcaattatga atttgatcgt gatctcgtaa
    86401 tacgttgctg ttattcttta attaattatc tgccaattta tttttagata gttacaggaa
    86461 atgtttatgc aaagagtggt ttgatatggt aagagtaata atttagatga agataaatat
    86521 atcaaacgta caccctagta gttattttta attaaacata tcgtccatga ggtgcggagt
    86581 cattctaatc aacttaatgt gttctgttta ttaagcattt cctataaata aacgactatc
    86641 aatacgttga tagttttcat taacatgcaa tattaattaa aatattaccc catgattgtt
    86701 gcaaatatgt catcataccc acctcgaaaa aaagagttgg tgcattctat acaaagttta
    86761 catgctcaag tagataaaat taatctttgc ctgaatgagt ttgaagaaat tcctgaggaa
    86821 ttagatggtt tttcaaaatt aaatccagtt attccagata aagattataa ggatgtgggc
    86881 aaatttatat ttccttgcgc taaaaatgat atgatcgtac ttacagatga tgatattatt
    86941 taccctcccg attatgtaga aaaaatgctc aatttttata attcctttgc aatattcaat
    87001 tgcattgttg ggattcatgg ctgtatatac atagatgcat ttgatggaga tcagtctaaa
    87061 agaaaagtat tttcatttac tcaagggcta ttgcgaccga gagttgtaaa tcaattaggt
    87121 acagggactg tttttcttaa ggcagatcaa ttaccatctt taaaatatat ggatggttct
    87181 caacgattcg tcgatgttag attttctcgc tatatgttag agaatgaaat tggtatgata
    87241 tgtgttccca gagaaaaaaa ctggctaaga gaggtctcat caggttcaat ggaaggactt
    87301 tggaacacat ttacaaaaaa atggccttta gacatcataa aagaaacaca agcaatcgca
    87361 ggatattcaa aacttaacct cgaattagtg tataatgtgg aagggtaaaa acttactttt
    87421 ttattcacat tcctgtattt tgtgttggtt tctgaagttt atagtataaa tacttgtttt
    87481 aaatagttgt acgttgatat tttgttatat acttatttaa accatttgtt ttatgatttt
    87541 gaaaaatatc agcgttagtt tggtagagtt tataattaag atttttgtct aaaagaaggt
    87601 ggtaacgcaa tatgtcaatt attaggaggt gctctgagtt atattgatat tgtttattga
    87661 tgaatggcta taccaaataa atcagatgtg ctattgagat atagatagtt tcatttagta
    87721 ttatcacata acgccaccta aattacatta cagatttgaa atatatgtct gcaatatcac
    87781 cattacgata aacgacagtg tttaaaataa agtaatcttg tagataataa agaggaaata
    87841 tgatgaataa attagtgcta gtcggacatc ctggctcaaa gtatcagata gttgaacatt
    87901 ttttgaaaga aattggcatg aactcaccaa attattctac aagtaataaa atttccccag
    87961 aatatatcac cgcttcatta tgtcaatttt atcaaacacc agaagttaat gatgtagtag
    88021 atgagagaga attctcagct gttcaagtct caaccatgtg ggatagcatg gttcttgaac
    88081 taatgatgaa caatctaaat aacaaacttt gggggtgggc agatccatct ataatatttt
    88141 ttcttgattt ttggaaaaat atagataaaa gcataaaatt catcatgata tatgatcacc
    88201 ctaaatataa tttaatgcgt tcagtaaata atgcccctct ctctttaaat ataaataata
    88261 gtgtagataa ctggattgca tataataaaa gattgcttga tttttttttg gagaataaag
    88321 aacgatgtgt gttgattaat tttgaggcgt ttcaaagcaa taagaaaaat attataaagc
    88381 cattgagtaa tattataaaa atagataatc taatgtctgc gcattacaaa aattcaatat
    88441 tgtttgatgt ggttgagaat aatgattata caaaatcaaa tgaaattgcc ctgcttgaaa
    88501 aatatacaac tttattttct ttaagtgcaa atgagactga aattacattt aatgatacaa
    88561 aggttagtga gtacttagta tctgaattaa taaaagaaag aaccgaggtt ctgaagcttt
    88621 ataatgagtt acaagcctat gcaaacctac cttatataga aacatcgaaa gataacgttt
    88681 cggctgaggc tgcattatgg gaggtagtcg aagagagaaa ttctatcttc aatattgtat
    88741 ctcatttggt gcaagagtca aaaaagaagg atgcagatat tgaattgact aaatctatat
    88801 ttaagaaaag acaattttta ttattgaaca ggattaatga gctaaaaaaa gaaaaggaag
    88861 aggtaattaa actttcaaaa ataaatcaca acgatgttgt gagacaagaa aaatatccag
    88921 atgatattga aaaaaaaata aatgacatac a
//
LOCUS       DAOFSV010000003        85713 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.003, whole genome shotgun sequence.
ACCESSION   DAOFSV010000003 DAOFSV010000000
VERSION     DAOFSV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.003"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(63..971)
                     /locus_tag="QBH27_000205"
     CDS             complement(63..971)
                     /locus_tag="QBH27_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDQ7012583.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            1349..1792
                     /gene="cmtB"
                     /locus_tag="QBH27_000206"
     CDS             1349..1792
                     /gene="cmtB"
                     /locus_tag="QBH27_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDQ7012584.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     SIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGKDEPIKLLIG
                     LSAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            1820..3208
                     /gene="cmtA"
                     /locus_tag="QBH27_000207"
     CDS             1820..3208
                     /gene="cmtA"
                     /locus_tag="QBH27_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDQ7012585.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLFCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFTESANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            3223..4500
                     /locus_tag="QBH27_000208"
     CDS             3223..4500
                     /locus_tag="QBH27_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ7012586.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVVVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            4497..5462
                     /gene="yggF"
                     /locus_tag="QBH27_000209"
     CDS             4497..5462
                     /gene="yggF"
                     /locus_tag="QBH27_000209"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDQ7012587.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            5484..5993
                     /gene="fumE"
                     /locus_tag="QBH27_000210"
     CDS             5484..5993
                     /gene="fumE"
                     /locus_tag="QBH27_000210"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDQ7012588.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            5990..6703
                     /locus_tag="QBH27_000211"
     CDS             5990..6703
                     /locus_tag="QBH27_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDQ7012589.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(6675..7352)
                     /locus_tag="QBH27_000212"
     CDS             complement(6675..7352)
                     /locus_tag="QBH27_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ7012590.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(7346..8023)
                     /locus_tag="QBH27_000213"
     CDS             complement(7346..8023)
                     /locus_tag="QBH27_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDQ7012591.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(8011..8718)
                     /locus_tag="QBH27_000214"
     CDS             complement(8011..8718)
                     /locus_tag="QBH27_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDQ7012592.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPHSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQCVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(8719..9297)
                     /locus_tag="QBH27_000215"
     CDS             complement(8719..9297)
                     /locus_tag="QBH27_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDQ7012593.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPIIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(9320..9751)
                     /locus_tag="QBH27_000216"
     CDS             complement(9320..9751)
                     /locus_tag="QBH27_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ7012594.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            10123..11142
                     /gene="epd"
                     /locus_tag="QBH27_000217"
     CDS             10123..11142
                     /gene="epd"
                     /locus_tag="QBH27_000217"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDQ7012595.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            11192..12355
                     /gene="pgk"
                     /locus_tag="QBH27_000218"
     CDS             11192..12355
                     /gene="pgk"
                     /locus_tag="QBH27_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDQ7012596.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            12569..13648
                     /gene="fbaA"
                     /locus_tag="QBH27_000219"
     CDS             12569..13648
                     /gene="fbaA"
                     /locus_tag="QBH27_000219"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDQ7012597.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            13839..14699
                     /gene="mscS"
                     /locus_tag="QBH27_000220"
     CDS             13839..14699
                     /gene="mscS"
                     /locus_tag="QBH27_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDQ7012598.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            14838..15473
                     /gene="argO"
                     /locus_tag="QBH27_000221"
     CDS             14838..15473
                     /gene="argO"
                     /locus_tag="QBH27_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDQ7012599.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            15566..16306
                     /locus_tag="QBH27_000222"
     CDS             15566..16306
                     /locus_tag="QBH27_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDQ7012600.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            16474..17370
                     /locus_tag="QBH27_000223"
                     /pseudo
     CDS             16474..17370
                     /locus_tag="QBH27_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(17367..18422)
                     /locus_tag="QBH27_000224"
     CDS             complement(17367..18422)
                     /locus_tag="QBH27_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDQ7012601.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLATHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDIKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISG
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(18625..19518)
                     /gene="argP"
                     /locus_tag="QBH27_000225"
     CDS             complement(18625..19518)
                     /gene="argP"
                     /locus_tag="QBH27_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDQ7012602.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19659..19889
                     /locus_tag="QBH27_000226"
     CDS             19659..19889
                     /locus_tag="QBH27_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012603.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            19945..20604
                     /gene="rpiA"
                     /locus_tag="QBH27_000227"
     CDS             19945..20604
                     /gene="rpiA"
                     /locus_tag="QBH27_000227"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDQ7012604.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20860..22092
                     /gene="serA"
                     /locus_tag="QBH27_000228"
     CDS             20860..22092
                     /gene="serA"
                     /locus_tag="QBH27_000228"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDQ7012605.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22321..22380
                     /gene="ibsC"
                     /locus_tag="QBH27_000229"
     CDS             22321..22380
                     /gene="ibsC"
                     /locus_tag="QBH27_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDQ7012606.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(22481..23083)
                     /locus_tag="QBH27_000230"
     CDS             complement(22481..23083)
                     /locus_tag="QBH27_000230"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDQ7012607.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23104..23287)
                     /gene="ssrS"
                     /locus_tag="QBH27_000231"
     ncRNA           complement(23104..23287)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="QBH27_000231"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23329..23658)
                     /gene="zapA"
                     /locus_tag="QBH27_000232"
     CDS             complement(23329..23658)
                     /gene="zapA"
                     /locus_tag="QBH27_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDQ7012608.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23826..24404
                     /gene="ygfB"
                     /locus_tag="QBH27_000233"
     CDS             23826..24404
                     /gene="ygfB"
                     /locus_tag="QBH27_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDQ7012609.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24430..25755
                     /gene="pepP"
                     /locus_tag="QBH27_000234"
     CDS             24430..25755
                     /gene="pepP"
                     /locus_tag="QBH27_000234"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDQ7012610.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25752..26930
                     /gene="ubiH"
                     /locus_tag="QBH27_000235"
                     /gene_synonym="visB"
     CDS             25752..26930
                     /gene="ubiH"
                     /locus_tag="QBH27_000235"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDQ7012611.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERREDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            26953..28155
                     /gene="ubiI"
                     /locus_tag="QBH27_000236"
                     /gene_synonym="visC"
     CDS             26953..28155
                     /gene="ubiI"
                     /locus_tag="QBH27_000236"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDQ7012612.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKALQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28229..28312)
                     /locus_tag="QBH27_000237"
     CDS             complement(28229..28312)
                     /locus_tag="QBH27_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012613.1"
                     /translation="MSKNTKSKIRGIRKYNAKSQVKLVYFK"
     gene            28603..29697
                     /gene="gcvT"
                     /locus_tag="QBH27_000238"
     CDS             28603..29697
                     /gene="gcvT"
                     /locus_tag="QBH27_000238"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDQ7012614.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            29721..30110
                     /gene="gcvH"
                     /locus_tag="QBH27_000239"
     CDS             29721..30110
                     /gene="gcvH"
                     /locus_tag="QBH27_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDQ7012615.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            30229..33102
                     /gene="gcvP"
                     /locus_tag="QBH27_000240"
     CDS             30229..33102
                     /gene="gcvP"
                     /locus_tag="QBH27_000240"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDQ7012616.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGALLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLAEWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLIRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(33268..34701)
                     /gene="bglA"
                     /locus_tag="QBH27_000241"
     CDS             complement(33268..34701)
                     /gene="bglA"
                     /locus_tag="QBH27_000241"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDQ7012617.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQKSMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            34746..35057
                     /locus_tag="QBH27_000242"
     CDS             34746..35057
                     /locus_tag="QBH27_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDQ7012618.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            35221..35880
                     /locus_tag="QBH27_000243"
     CDS             35221..35880
                     /locus_tag="QBH27_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDQ7012619.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(35957..36937)
                     /gene="ygfZ"
                     /locus_tag="QBH27_000244"
     CDS             complement(35957..36937)
                     /gene="ygfZ"
                     /locus_tag="QBH27_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDQ7012620.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARTALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            37180..37446
                     /gene="sdhE"
                     /locus_tag="QBH27_000245"
     CDS             37180..37446
                     /gene="sdhE"
                     /locus_tag="QBH27_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDQ7012621.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            37427..37834
                     /locus_tag="QBH27_000246"
     CDS             37427..37834
                     /locus_tag="QBH27_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDQ7012622.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(37874..38395)
                     /gene="fldB"
                     /locus_tag="QBH27_000247"
     CDS             complement(37874..38395)
                     /gene="fldB"
                     /locus_tag="QBH27_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDQ7012623.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            38507..39403
                     /gene="xerD"
                     /locus_tag="QBH27_000248"
     CDS             38507..39403
                     /gene="xerD"
                     /locus_tag="QBH27_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDQ7012624.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            39428..40138
                     /gene="dsbC"
                     /locus_tag="QBH27_000249"
     CDS             39428..40138
                     /gene="dsbC"
                     /locus_tag="QBH27_000249"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDQ7012625.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            40144..41877
                     /gene="recJ"
                     /locus_tag="QBH27_000250"
     CDS             40144..41877
                     /gene="recJ"
                     /locus_tag="QBH27_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDQ7012626.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            41968..43066
                     /gene="prfB"
                     /locus_tag="QBH27_000251"
     CDS             join(41968..42042,42044..43066)
                     /gene="prfB"
                     /locus_tag="QBH27_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDQ7012627.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            43076..44593
                     /gene="lysS"
                     /locus_tag="QBH27_000252"
     CDS             43076..44593
                     /gene="lysS"
                     /locus_tag="QBH27_000252"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDQ7012628.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(44636..45184)
                     /gene="idi"
                     /locus_tag="QBH27_000253"
     CDS             complement(44636..45184)
                     /gene="idi"
                     /locus_tag="QBH27_000253"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDQ7012629.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            45239..45310
                     /locus_tag="QBH27_000254"
     CDS             45239..45310
                     /locus_tag="QBH27_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012630.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(45307..45432)
                     /locus_tag="QBH27_000255"
     CDS             complement(45307..45432)
                     /locus_tag="QBH27_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012631.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(45434..46882)
                     /gene="uacT"
                     /locus_tag="QBH27_000256"
     CDS             complement(45434..46882)
                     /gene="uacT"
                     /locus_tag="QBH27_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDQ7012632.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            47318..49237
                     /gene="ygfT"
                     /locus_tag="QBH27_000257"
     CDS             47318..49237
                     /gene="ygfT"
                     /locus_tag="QBH27_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDQ7012633.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            49237..49725
                     /locus_tag="QBH27_000258"
     CDS             49237..49725
                     /locus_tag="QBH27_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDQ7012634.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(49761..51128)
                     /gene="ghxQ"
                     /locus_tag="QBH27_000259"
                     /gene_synonym="ygfQ"
     CDS             complement(49761..51128)
                     /gene="ghxQ"
                     /locus_tag="QBH27_000259"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDQ7012635.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(51164..52480)
                     /gene="guaD"
                     /locus_tag="QBH27_000260"
     CDS             complement(51164..52480)
                     /gene="guaD"
                     /locus_tag="QBH27_000260"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDQ7012636.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(52498..53898)
                     /gene="xanQ"
                     /locus_tag="QBH27_000261"
     CDS             complement(52498..53898)
                     /gene="xanQ"
                     /locus_tag="QBH27_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDQ7012637.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGISYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(54063..56933)
                     /locus_tag="QBH27_000262"
     CDS             complement(54063..56933)
                     /locus_tag="QBH27_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDQ7012638.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(56930..57709)
                     /locus_tag="QBH27_000263"
     CDS             complement(56930..57709)
                     /locus_tag="QBH27_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDQ7012639.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(57760..59088)
                     /gene="ssnA"
                     /locus_tag="QBH27_000264"
     CDS             complement(57760..59088)
                     /gene="ssnA"
                     /locus_tag="QBH27_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDQ7012640.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFTRQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIVLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(59091..62189)
                     /gene="ygfK"
                     /locus_tag="QBH27_000265"
     CDS             complement(59091..62189)
                     /gene="ygfK"
                     /locus_tag="QBH27_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDQ7012641.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKSKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(62511..63089)
                     /gene="mocA"
                     /locus_tag="QBH27_000266"
     CDS             complement(62511..63089)
                     /gene="mocA"
                     /locus_tag="QBH27_000266"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDQ7012642.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            63193..63963
                     /gene="yqeC"
                     /locus_tag="QBH27_000267"
     CDS             63193..63963
                     /gene="yqeC"
                     /locus_tag="QBH27_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDQ7012643.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            64011..65636
                     /locus_tag="QBH27_000268"
     CDS             64011..65636
                     /locus_tag="QBH27_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDQ7012644.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLHQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(65677..66609)
                     /gene="arcC"
                     /locus_tag="QBH27_000269"
     CDS             complement(65677..66609)
                     /gene="arcC"
                     /locus_tag="QBH27_000269"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDQ7012645.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(66657..68042)
                     /gene="hyuA"
                     /locus_tag="QBH27_000270"
     CDS             complement(66657..68042)
                     /gene="hyuA"
                     /locus_tag="QBH27_000270"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDQ7012646.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(68095..69306)
                     /locus_tag="QBH27_000271"
     CDS             complement(68095..69306)
                     /locus_tag="QBH27_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDQ7012647.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(69364..70560)
                     /gene="dpaL"
                     /locus_tag="QBH27_000272"
     CDS             complement(69364..70560)
                     /gene="dpaL"
                     /locus_tag="QBH27_000272"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDQ7012648.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(70618..71805)
                     /gene="ygeW"
                     /locus_tag="QBH27_000273"
     CDS             complement(70618..71805)
                     /gene="ygeW"
                     /locus_tag="QBH27_000273"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDQ7012649.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            72284..74062
                     /locus_tag="QBH27_000274"
     CDS             72284..74062
                     /locus_tag="QBH27_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDQ7012650.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEASEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(74101..74580)
                     /gene="xdhC"
                     /locus_tag="QBH27_000275"
     CDS             complement(74101..74580)
                     /gene="xdhC"
                     /locus_tag="QBH27_000275"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDQ7012651.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(74577..75455)
                     /gene="xdhB"
                     /locus_tag="QBH27_000276"
     CDS             complement(74577..75455)
                     /gene="xdhB"
                     /locus_tag="QBH27_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDQ7012652.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(75466..77763)
                     /gene="xdhA"
                     /locus_tag="QBH27_000277"
     CDS             complement(75466..77763)
                     /gene="xdhA"
                     /locus_tag="QBH27_000277"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDQ7012653.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAVQLVSIEWQELPVITSSEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            78177..78932
                     /locus_tag="QBH27_000278"
     CDS             78177..78932
                     /locus_tag="QBH27_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDQ7012654.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            79011..79084
                     /locus_tag="QBH27_000279"
     tRNA            79011..79084
                     /locus_tag="QBH27_000279"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79043..79045,aa:Gly,seq:ccc)
     gene            complement(79401..80630)
                     /locus_tag="QBH27_000280"
     CDS             complement(79401..80630)
                     /locus_tag="QBH27_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDQ7012655.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            80885..82066
                     /locus_tag="QBH27_000281"
     CDS             80885..82066
                     /locus_tag="QBH27_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDQ7012656.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            82353..83189
                     /gene="kduI"
                     /locus_tag="QBH27_000282"
     CDS             82353..83189
                     /gene="kduI"
                     /locus_tag="QBH27_000282"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDQ7012657.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            83219..83980
                     /gene="kduD"
                     /locus_tag="QBH27_000283"
     CDS             83219..83980
                     /gene="kduD"
                     /locus_tag="QBH27_000283"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDQ7012658.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            84295..85713
                     /gene="araE"
                     /locus_tag="QBH27_000284"
     CDS             84295..85713
                     /gene="araE"
                     /locus_tag="QBH27_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDQ7012659.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        20936 a        20946 c        22538 g        21293 t
ORIGIN      
        1 gtagcgcatc aggcagtttt gtctttgcta tcagtctcaa gggccgcgaa agcggccttt
       61 ttttacttag tagagataaa cggaactgtg ctgccgggga tcatggtatc gggcagttta
      121 ccgtcccaac gctcagcagt ggtgagggca acaagtccgg gattctcccg cagagcttca
      181 ccgcgcaggc ggatagcttc tgcttcagcc gcacttttta aacgaatagt ttcagcctct
      241 gccgcgcctc ttacgcgaat ggtctcagct tctgctttgg ctgccgccag tttactgtct
      301 gcttctgcct gggcctgagt tactgctatt tgcgcttgga ttttctctgt ttccagattt
      361 tgcttacgtg tggcaatggc gacttctgct ttcattcggt cttcaatcga cttttcataa
      421 gcgtcagaaa aatcaatatt ttcaatctgg acaccatcaa tcaccaccgg gccaacaacg
      481 gctttacgca ttgcattttg cagatcctga accagttttg tacgatcctg taccgcgctg
      541 atggcggtat attggccgaa aacgttctcc agttgagttg gcaactgacg gacaatcaga
      601 cgatctttta atgcttcaat ggtgttgtag gtggtataaa ccgcgcctgc ttctgaaggt
      661 ttaatgtgga agctgactga taccgtcatt tgtgccggtt gctggtcgcg gctgtaggcc
      721 tgtaatccct gatagaccac agcctgatta cgcgtggaaa ttttctctac actttccata
      781 aaagggatct taaaacccag ccccggctcg gcaactttta cgatttttcc gtagcgcagc
      841 aaaatgccgc gttcaccttc attaacggtg taatacgaga gaaatggcag cacaaccacg
      901 gcaagcacgc caatggcgat agcaatagat ttttgtgggc gaaatgaggt tattgaaaca
      961 ggtgctttca tatgaatctt ccttgagtaa agtatgggaa tatgtcacat ggggggagga
     1021 gaaaacagct ggcaggtgag aattcagaat ctataaaggg gtatagcgaa aaaacattgc
     1081 attagaaaag tatgctttcg gacattcaat aaataaatag agatatgcat cacaaataca
     1141 ataacaaaca aaatatttat tttgtgataa acataacaaa tacaaaattg actttaaaaa
     1201 tatcaatttc atgttgttgt tatttaaagt tttattattt ttactcatga ggtttttctt
     1261 ctcgcgtgcc caattgcttt cattaaactg cacgagtatt ttatttttac cgccggacgg
     1321 taccctaaaa ataaaataag gataatttat gcggcttatt gattattttc cagaatcatc
     1381 aatctcagtt atacattcag caaaagactg gcaggaagct atcgatttct cgatggcatc
     1441 attgctggat aaaaactata tcagcgagaa ttacattcaa tctattaaag actccaccat
     1501 cagcaatggt ccttattata ttctcgcacc aggcgtggca atgcctcatg cgcgaccgga
     1561 atgtggggcg cttaaaaccg ggatgtcttt gacgttactt aaacaaggcg tttatttccc
     1621 ggggaaggac gaaccgatta aattactcat cgggctttct gctgccgatg ccaattcgca
     1681 cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac tcgaacaact
     1741 cttaacagca tcatcagaaa aacaattagc ggacattatc agccgcggat aattactctc
     1801 tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc aggcttttgg
     1861 gggctttttg actgcaatgg tcatccccaa tatcggtgct tttattgcct ggggatttat
     1921 aacggcatta tttattccca ccggctggct gccgaatgaa catttcgcca aaattgttgg
     1981 tccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc atctggtcgg
     2041 cggtaaacgc ggcgcggtca tgggcggaat tggtacgatt ggtgtgatcg tcggcgctga
     2101 aatcccgatg ttccttggct cgatgattat ggggccgctc ggtggtctgg tgattaaata
     2161 tatcgacaag tcgctggaaa aacgcatacc tgctggtttt gagatggtta tcaataactt
     2221 ctcattaggt atcgcgggga tgctcttttg tctgctgggt tttgaagtta tcggtccggc
     2281 ggtgttaatt gctaatactt tcgtcaaaga gtgtattgag gcgctggtac atgcgggtta
     2341 tctgcctttg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctta ataatgcgat
     2401 tgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg gtaaatccat
     2461 cttcttcatg gtggcctcca acccaggtcc tggtctgggg ctgctgctgg cgtttacctt
     2521 gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc acttcctcgg
     2581 tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca ttattgccat
     2641 gattgcgggc ggtatgtctg gcacctggat gtttaactta ctggacggtg gtctggtggc
     2701 tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag gctcgttcct
     2761 ggcgacaatt gccggtgtta cggtaggtac cctggtgtcc tttgctatca cttcgctgat
     2821 actgaagatg gaaaaaacgg tggaaacgaa gagcgaagat gagtttaccg agtcagccaa
     2881 tgcggttaag gcgatgaaac aagaaggtgc attctcgtta agccgtgtta agcgtattgc
     2941 ctttgtttgc gatgcaggga tgggctccag tgcgatgggc gcaaccacct tccgtaaacg
     3001 cctggaaaaa gcggggctgg caatagaagt aaaacattac gccatagaaa acgtgcctgc
     3061 ggatgcggat atcgtcgtta ctcacgccag tctggaaggg cgcgtgaaac gtgtgacgga
     3121 taaaccactg atattgatta ataactatat tggcgatcca aaactcgaca ctttatttaa
     3181 tcaattaacc gctgaacata aacactgatt acagaggtaa aaatgaaaac caaagttgct
     3241 gctatttatg gcaagcggga tgtccgtctg cgcgaatttg aactgccaga aattactgat
     3301 aatgaactgt tagtgagtgt aatttctgac agcgtttgtt tatccacctg gaaagcggcg
     3361 ttgcttggta gtgaacataa acgcgtcccc gacgatttag aaaatcatcc ggttattacc
     3421 gggcatgaat gtgctggggt tgttgtcgaa gtgggtaaaa atctcactgg caaatataaa
     3481 aaagggcagc gttttgtatt gcaaccggcg atggggctac caagcggata ttcagccgga
     3541 tacagctacg aatattttgg tggcaatgcc acctatatga ttattcccga aatcgcgatt
     3601 aatttaggct gtgtattacc gtatcacggc tcttattttg ctgcggcgtc gctggcagag
     3661 cctatgtgct gcattattgg tgcttatcat gccaattatc acaccacgca atatgtttat
     3721 gagcatcgta tgggcgtcaa acctggcggc aatattgccc tgctggcgtg tgctggcccg
     3781 atgggcattg gcgctatcga ttacgccatt aacggcggca tacaaccggc gcgggtggtg
     3841 gtagtcgata tcgacgacaa acggctggcg caggtgcaga agttgctgcc ggtggagctg
     3901 gcggccagta aaggcattga gctggtgtat atgaatacca aagggatgag cgatccggtc
     3961 cagacgctgc gggcgctgac ggatgatgcc ggatttgatg acgtatttgt ctatgcagcg
     4021 gtgccttcag tcgtcgagat ggctgatgaa ttactggctg aagatggctg cctgaacttc
     4081 tttgccggtc cgacggataa aaacttcaaa gtgccgttta atttctacaa cgtccattac
     4141 aacagtacgc acattgtcgg cacgtctggc ggttccacgg acgacatgaa agaggcgatt
     4201 gcccttagcg ccacggggca gttacagccg tcgtttatgg tgacccatat tggtggcctg
     4261 gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc ctggcggtaa aaaactcatt
     4321 tataacggcg tgaccatgcc gctgaccgcc attgccgatt ttgccgaaaa aggcaaaacc
     4381 gatccgctgt ttaaagagct ggcgcggctg gttgagaaaa cgcacggtat ctggaatgaa
     4441 caggccgaga aatatctgct ggcacaattt ggcgttgata ttggggaggc cgcgcaatga
     4501 tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct gcctggccgc
     4561 aaaccggctg tggcgacaaa aataaaattg atggccttgc ggtcaccgcg atgcgccagg
     4621 cattaaacga cgttgctttt cgtgggcgag tggttatcgg cgagggggaa attgaccatg
     4681 cgccgatgct gtggattggc gaagaggtgg gcaaaggcga tgggccagaa gtcgatatcg
     4741 cggtcgatcc cattgaaggc acgcggatgg tggcgatggg gcagagcaat gcgctggcgg
     4801 tcatggcttt cgcccctcga gatagcctgt tacatgcgcc cgatatgtat atgaaaaagc
     4861 tggttgttaa tcgactggct gccggggcga tagatctgtc tttgcctctg gcggacaact
     4921 tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa gctgcgcatg gttacgctgg
     4981 ataaaccgcg cctgagtgcc gcaattgagg aagcaacaca gctcggcgtg aaggtttttg
     5041 ccctgccgga tggcgatgtc gccgccagcg tgctgacttg ctggcaggat aatccttacg
     5101 atgtgatgta caccatcggt ggcgcaccgg aaggtgttat ttctgcctgt gccgttaaag
     5161 cattaggcgg ggatatgcag gccgagctga ttgacttttg tcaggctaaa ggggattaca
     5221 cggaaaatcg gcagattgca gagcaggagc gcaagcgttg caaggcaatg ggcgttgacg
     5281 ttaaccgtgt atactcgctc gatgaactgg tgagggggaa cgatatcctc tttagcgcta
     5341 cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca gacggcgaat ggggtgcgga
     5401 cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa tataatagac tccctgcatt
     5461 gatggtgatc tatcggaaga ccaatggcga cgctgacaga agatgatgtg cttgagcaac
     5521 tgaatgcaca ggacaattta ttttcattta tgaaaactgc gcattctatt ttgctccagg
     5581 ggatacgcca gtttctgccg tcgctgtttg tcgataacga tgaagagatc gtcgaatatg
     5641 cagtgaagcc gttactcgcc caaagcggcc cgcttgacga tattgatgtt gcgctgcgtt
     5701 tgatttatgc gctggggaaa atggataaat ggctgtacgc cgatatcacg catttttccc
     5761 agttttggca ttacctgaac gaacaggatg aaacgcccag atttgctgat gacataacct
     5821 gggattttat cagcaacgtc aatagcataa cccgcaatgc gacgctctat gacgcgttga
     5881 aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc gcgttttagc gggatggtca
     5941 aaacggcgct gacgctggca gtaacgacca ccttaaagga attaacgccg tgaaaatcga
     6001 actgacggtg aatggactaa aggtacaggc gcagtactct gatgatgaaa ttgaaaatgt
     6061 acataaacca ttgctgcgta tgctggcggc cttacagaca gtaaatccgc agcggcgtac
     6121 ggtggttttt ctttgcgctc cgccggggac gggtaaatct accctaacca ccttctggga
     6181 atatctcgcg caacaagacc cggaactgcc cgctattcag acgctgccga tggatggttt
     6241 tcaccattac aatagctggc tggatgtgca tcaattgcga cccttcaaag gcgcaccaga
     6301 gacattcaac gttgcgaaac tggcggaaaa tctgtgccgg gttgtggaag gggattgtac
     6361 gtggccgcag tacgatcgac aaaagcatga tcctgttgaa gatgtgttgc acgttaccgc
     6421 accactcgtc atcgtcgaag gaaactggtt gttgttggac gacgagaagt ggtgccagct
     6481 tgcgcaattt tgtgatttct cgatctttat caaagctccc gcatctgcgt tgcgggagcg
     6541 tctggtgggg cgcaagctgg cgggagggtt atcgctggca gacgcagaag ccttttatga
     6601 ccgtaccgac gggccgaatg ttcgccgggt gctggaagag agtctgcctg caaatttgac
     6661 cttaatgatg accgctacgg gtgaatatcg tctgatggat taacgttcct gattacacca
     6721 tcttccagtc gcaccacacg agagaaatga cgccgggtaa atgcggcgtc atgactgatt
     6781 gctacgacgc tcgtgcctcg ctggcggcac ttttccagcc agctttcaaa cacgctcaac
     6841 caattttcgt caaaatcccg actcggctcg tccagtagca atagtggcgg agagagtgct
     6901 tccaggcagg cgacggcgac catccgtcgc tgcgcactat gcagatctaa cggatgcgcg
     6961 ctggcagtgt ctgctaactg acagcattgc agggcagcat tggtacgttg agtgatttca
     7021 tccgccgggc atttttgcag tatcaaacca aatgcgattt cgtcagcaac ggtgctgtga
     7081 aaaagctgat tttccgcttc ctgaaacagt acgccaatct ttgccgcgcg ctggcggttt
     7141 ttcaggtttt tcatcgcttg ttgttgcagc ataaccgtac cggctgtggg cgtaagaaga
     7201 cctgccatta ctcgtaacag cgtcgatttt cccgcgccat tgtcgccggt cagcgccagc
     7261 cattcacctt gtttgagttg cagagaaatg tcgcaaagac aatccgtagc cgcgccaggc
     7321 cagcgataag atatctgatt taacgttaac atagtggctg gagcgctccg ttttgcaatt
     7381 gccacgcttg ctggcagtgg ttcaggaaag gtgtgtgatg acgttcaaaa agaataatca
     7441 gcgaaccacg ttcaagcgcc cagtgttgta agcgctgcaa cagcatttcg cgggcctgcg
     7501 gcgtcaggcg gctaaaggct tcatcaagaa ttaagagctt tggctgcatc gcgatggcgc
     7561 aggcgatgac cacacgctgt gtctctccac cggagagtgt tgccgggtga cgatggcgca
     7621 acggctggca ctcggtcagg gccagggcgg cgtcaatccg cgccataatc tctttttcag
     7681 caagacacag attttctggc ccaaaggcaa cttcttcttc cacgctaaag gtacagccgg
     7741 agagctgcaa atagggcgat tgctggacga gctggatggt ggcggattgt tcattaagcg
     7801 ggagacgccc aatgggcgta ccgagaagcg tccccgtgcc ggttatctct cctggtagaa
     7861 agtccggata ccagcccgcc atcagctgtg ccagcgtact tttgccactg ccgttgtcac
     7921 caaagatggc gaccatcccc ggtttgacat agtgaaagtc ataacagaac ggaggatgcg
     7981 ttgagtgtgt cgggcaatag cgaaactgtt ctaacgtaac catatccacg ctccgaattc
     8041 agccagcatt agcaatatca tggcgtaacg tgctacgcat tgtagggtgc tatcggctgg
     8101 tgcccataac gtagtgcgcc tattgttgat tcgaaatgcc cgcatatcca gcgccgcacc
     8161 acggacagca agatcgttta gcgcattgtg tgttagcgga ataattaacg cgggcatcgc
     8221 acgtaaacgc tgataccagc cctcatccag cggcacgccg cgggcgcact gcgcttcatg
     8281 aataatcgcc aattgccgtt ttaactgctc aacgaccaat aacggcccgg caaacagata
     8341 agcgacgccc ggcggcaggc gagaagcaaa caaagcgcgg ataaaacgtt gcacggggac
     8401 gtactgcatc cacaactgtg aggttgaaac aatcgccaga atccgcaacc agagcgtaat
     8461 agcatgcgcc caccgttctg gagagtgtgg cgtgccgctt agccattctg tcagccagcc
     8521 accgtgcacc agccacagtc cagcccccag cgagaacatc agccagacga catattttgc
     8581 ccgccgccgc gtggccttta gcgcaataag gcagaaaaag gtcgccgcgc tatagactgg
     8641 caatattgtc tgtgtaggca ggataagcgt ggtgcaggcc gccagcgccc acagcgttaa
     8701 ggatgtaaaa ggatgcattt agcgcacggc agccattgcc ggaaaatggc gcgtggtgcg
     8761 aatcggcagc tgacgcagta aaagccagac tatcaccgct gtaagaattt tatcgaccag
     8821 gttcgcgcct atcaccgtaa tcgccaccga ctccaccagg ttttgcccca tcgagtgcat
     8881 ccaggcaacg aacaaatcgg ctccgcttcc tgtcacgccg ccgaacagcg cagtacgcag
     8941 cggtacagcg acgaccgtta ccgcaagcgt aataatcacg ccgctaacaa cgacttttgg
     9001 cagggtgcga aaccaacccg ctcgcgccag ccaaccggcg accaggccga tgaccatcgc
     9061 gacgggcgca aatgcggcgg cgatcgggtc ggtcagcaat ccccagagta aattggtcag
     9121 taaaccggtc agcatcccga ttatcggacc aagcagtacc gcgctaataa gtgtgccgat
     9181 ggagtcgaga aaaataggta atttaaccat gctggccagt tgcccgccga tcatattgat
     9241 ggcgatagag ataacgatca acaccagtgc ctgactggaa aagtggcgac gcgccataag
     9301 aaaacctctc tgaaaatagt tatttaacgg gggaaatatg caactcgtcg taaccagaaa
     9361 gcgcgcaaag ctggcggcta aacgccagtt cctcaaggca gccgatcacc agttcaagcg
     9421 tggtccgcac ggtacagccg ggcatcatat gaccgccaag catcgtgccg tgtggatcgg
     9481 aaacgcagag atgaagatgt tcaccactct gttccagcgt gccgttcaat gcgatgactt
     9541 cgaacttacc gcttagcagg gcagtgtttt cttgcccggc atagcgtaag gcgacgtcag
     9601 tcagactgcc ggtacaacct gcaatccacg cggcgtgaag ttgttgttgc tgcgcgaagg
     9661 cgcgtaattg ggagagaact tcctgcccgg ggagcaggcg cagcgcgtaa aagcgtgcgg
     9721 aggaagaata tgggtagggc aatgtcatca ttggagtact ccgaaactcg gacgtattgc
     9781 ggataatatc gacgctgcat cagtcatatt ctgatataag tcagggtttg tgaccgcaca
     9841 acgaaaagat attgctacgc ttattcgtga ttctgcaaag tggaataaat tccacagggg
     9901 cggggatgaa aacgtgacat aagtcagata attgtcttct ttgactggac aaacattcct
     9961 tttattccac gtttcgctta tcctagctga agcgtttcag tcgattaaat gttcgacaat
    10021 taaccaatca gtcgcagttt gcgacaggta aggtttcccc gaacgatttg ctggattact
    10081 ctgtctggca aatttgctca gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg
    10141 ataaatggct tcggtcgcat cgggcgtaat gtggttcgtg ctttgtatga atccggacgc
    10201 cgtgcggaaa ttaccgtggt ggcaatcaac gaactggcgg atgctgcggg catggcgcat
    10261 ttgttgaaat atgacaccag ccatggccgt tttgcatggg aagtacgaca ggaacgcgat
    10321 caactttttg ttggtgatga cgccatccgt gtattgcatg aacgttcact gcaatcgctc
    10381 ccctggcgtg aacttggcgt tgatgtagtc ctcgactgca ccggcgtata tggctcccgc
    10441 gagcatggtg aagcacacat tgccgccggg gctaaaaaag tgctcttttc acatcctggc
    10501 agtaacgatc tcgacgcgac cgttgtttat ggcgtcaatc aggatcaact tcgtgcggaa
    10561 caccgcatcg tttctaatgc ttcctgtacc acgaattgca taattcccgt catcaaattg
    10621 ttagatgatg cgtacggtat tgagtctggc actgtgacca caattcactc cgccatgcat
    10681 gatcaacagg ttattgatgc ataccatcct gacctgcgtc gcactcgggc agccagtcag
    10741 tcgatcattc cggtcgatac taaactggcc gccggtatca cacgattttt tccgcaattt
    10801 aacgatcgct ttgaggcgat tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat
    10861 ttaagcgtga cggtgaagaa acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa
    10921 gcagcacaag gtgcatttca tggtatagtt gactatacgg aattgccgtt ggtctctgta
    10981 gattttaacc acgatccgca cagtgccatt gtcgatggca ctcaaacccg ggtcagtggc
    11041 gcacacctga tcaaaacgtt ggtctggtgc gataacgaat ggggctttgc taaccgaatg
    11101 ctcgacacga cgttagctat ggctactgtt gctttcaggt aagacgcaag cagcgtctac
    11161 aaaactttta gaatcaacga gaggattcac catgtctgta attaagatga ccgatctgga
    11221 tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa
    11281 agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca
    11341 aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg agtacaacga
    11401 agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta acccggttcg
    11461 tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg ttgttctgga
    11521 aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa actctgtcca aaaaatacgc
    11581 tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg cgcaggcttc
    11641 tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga
    11701 actggacgcg ctgggtaaag cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg
    11761 tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa tcgctgacca
    11821 gctgatcgtt ggtggtggta ttgctaacac ctttattgcg gcacaaggcc acgatgtggg
    11881 taaatccctg tacgaagctg acctggttga cgaagctaaa cgtctgctga ccacctgcaa
    11941 catcccggtt ccgtctgatg ttcgcgtagc aaccgagttc tctgaaactg caccggctac
    12001 cctgaaatct gttaacgatg tgaaagctga cgagcagatc ctggatatcg gtgatgcttc
    12061 cgctcaggaa ctggctgaaa tcctgaagaa tgcgaaaacc attctgtgga acggtccggt
    12121 tggcgtgttc gaattcccga acttccgcaa aggtactgaa atcgtggcta acgctatcgc
    12181 agacagcgaa gcgttctcca tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt
    12241 cggcattgct gacaaaatct cctacatctc cactggcggc ggcgcattcc tcgaattcgt
    12301 ggaaggtaaa gtactgcctg cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat
    12361 cacagggcag ggaaacctgc ccttgtttca gcgcgctttt agagcacgca ctttttcata
    12421 ctctaaataa ttcgagttgc aggaaggcga caagagagtg aatcccagga gcttacataa
    12481 gtaagtgatt ggggtgagcg aacgcagacg cagcacatgc aacttgaagt atgacgagta
    12541 taaggcccga cgatacagga caagagacat gtctaagatt tttgatttcg taaaacctgg
    12601 cgtaatcact ggtgatgacg tacagaaagt tttccaggta gcaaaagaaa acaacttcgc
    12661 actgccagca gtaaactgcg tcggtactga ctccatcaac gccgtgctgg aaaccgctgc
    12721 taaagttaaa gcgccggtta tcgttcagtt ctccaacggt ggtgcttcct ttatcgctgg
    12781 taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc ctgggcgcga tctctggtgc
    12841 gcatcacgtt caccagatgg ctgaacatta tggtgttccg gttatcctgc acaccgacca
    12901 ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg gacgcgggtg aaaaacactt
    12961 cgcagctacc ggcaagccgc tgttctcttc tcacatgatc gacctgtctg aagaatctct
    13021 gcaagagaac atcgaaatct gctctaaata cctggagcgc atgtccaaaa tcggcatgac
    13081 tctggaaatc gaactgggtt gcaccggtgg tgaagaagac ggcgtggaca acagccacat
    13141 ggacgcttct gcactgtaca cccagccgga agacgttgat tatgcgtata ccgagctgag
    13201 caaaatcagc ccgcgtttca ccatcgcagc gtccttcggt aacgtacacg gtgtttacaa
    13261 gccgggtaac gttgttctga ctccgaccat cctgcgcgac tctcaggaat atgtttctaa
    13321 gaaacacaac ctgccgcaca acagcctgaa cttcgtattc cacggtggtt ccggttctac
    13381 tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg aaaatgaaca tcgataccga
    13441 tacccaatgg gcaacctggg aaggcgttct gaactactac aaagcgaacg aagcttacct
    13501 gcagggtcag ctgggtaacc cgaaaggcga agatcagccg aacaagaaat actacgatcc
    13561 gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct cgtctggaaa aagcattcca
    13621 ggaactgaac gcgatcgacg ttctgtaagt cattcttttc tgcttatctc aaggcccgct
    13681 ctgcgggtct ttttttcgcc aaaagcaagg tgatgcagat gagaaatctg tgatctattt
    13741 ggcaaaatta tgctttattg tttacccttg tcagactgcc cgccataagg cggcggagtg
    13801 tatttctcca ttttgagtca gttgaaaagg aatattgaat ggaagatttg aatgttgtcg
    13861 atagcataaa cggcgcggga agctggctgg tggctaacca ggcgctgctg ctaagttatg
    13921 cagtaaacat cgtggcggca ctcgcgatca tcatcgttgg tttgattatc gcgcggatga
    13981 tttccaacgc ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact gttgctgatt
    14041 ttctttctgc attagtccgt tacggaatta tcgcctttac gctaatcgct gcactggggc
    14101 gcgtgggcgt acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc ttagctgttg
    14161 gtctggcttt gcaggggtca ctgtctaacc tggccgctgg cgtgttactt gtcatgttcc
    14221 gcccgttccg tgccggtgaa tatgttgacc tcggcggcgt agccggcact gtgctgagtg
    14281 tgcagatttt ctccaccacc atgcgtactg cagacggtaa aattatcgtt attccgaatg
    14341 gtaaaattat tgccgggaat attattaact tctcccgcga gccagctcgc cgtaacgaat
    14401 ttattattgg cgtggcgtat gattccgata tcgatcaggt taagcagatc ctgaccgata
    14461 ttatccagtc tgaagatcgc attttgaaag atcgcgaaat gactgtgcgt ctgaacgaac
    14521 ttggtgcatc gtcgattaat ttcgtggtcc gagtctggag caacagcggc gatctgcaaa
    14581 acgtgtactg ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct
    14641 tcccgtatcc gcaaatggat gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat
    14701 caacgctgag gccagataat actctctggc ctctctctta ttagtttttc tgattgccaa
    14761 ttaatattat caatttccgc taataacaat cccgcgatat agtctctgca tcagatactt
    14821 aattcggaat atctaacgtg ttttcttatt actttcaagg tcttgcactt ggggcggcta
    14881 tgatcctacc gctcggtcca caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt
    14941 accacattat gattgcctta ctttgtgcta tcagcgattt ggtcctgatt tgtgcgggga
    15001 tttttggtgg cagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg
    15061 gcggcgtagc cttcttgctg tggtatggtt ttggcgcttt taaaacagca atgagcagta
    15121 acattgagtt agccagtgcc gaagtcctga aacaaggcag atggaaaatt atcgccacca
    15181 tgttggcagt gacctggctg aatccgcatg tttacctgga tacttttgtt gtgctgggca
    15241 gccttggcgg gcaacttgat gtggaaccaa aacgttggtt tgcgctcggg acaattagcg
    15301 cctctttcct gtggttcttt ggtctggcta ttctcgcagc ctggctggca ccgcgcctgc
    15361 gcacggcaaa atcacagcgc attatcaatc tggtagtggg atgtgttatg tggtttattg
    15421 ccttgcagct ggcgagagac ggtattgctc atgcacaagc cttgttcagt taggcgctat
    15481 ctgatggaaa aataaaacag aggcgctaag cttgcctcca gaggtcctga attttgggca
    15541 agtcaattaa cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat
    15601 gggtattagc gggatggcag cgcaggctaa cgaattgccg gatggaccgc atattgtcac
    15661 ctccggtacg gcaagcgtgg atgcggtgcc agacattgcc actcttgcga ttgaagttaa
    15721 cgtggccgcg aaggatgccg ctactgccaa gaaacaggca gatgagcgcg tcgcacaata
    15781 catttccttc cttgagctca atcagatcgc gaaaaaagat atcagctcag cgaacttacg
    15841 cactcagcca gattatgatt atcaggatgg taaaagtatc ctgaaaggct accgcgcagt
    15901 gagaacggtg gaagtcacgc tccgtcagtt agacaaactg aattccttgc tggacggcgc
    15961 gctaaaggcg ggtcttaacg aaattcgttc tgtgtcgctg ggcgtggcgc agccggatgc
    16021 ctataaagac aaagcgcgta aggcagcgat tgataacgcg attcatcagg cacaggaact
    16081 ggcgaatggc tttaatcgta aactggggcc ggtatatagc gtgcgctacc atgtttccaa
    16141 ctatcagccc agcccaatgg tgcggatgat gaaagctgat gctgcaccgg tgtccgcaca
    16201 ggaaacttac gagcaggccg ctattcagtt tgatgatcag gtcgatgtag tcttccagtt
    16261 agaacctgtg gatcaacaac ctgctaaaac acctgcagca caataatact tgtagcccga
    16321 cgtattcgtc gggctaatgc ctttacccga tattgcctcc tttttctgta attttctgta
    16381 aaaccatgac gtaatcacct atctttgctc gcggtttcat caaggatagg ctgactgaaa
    16441 cggttaagcg gacaaggatg cgggagaggg gggatggaca tttttatttc gaaaaagatg
    16501 cgcaatttca ttttattagc gcaaaccaat aatattgccc gggcggcaga aaaaatccat
    16561 atgacggctt cgccatttgg caaaagcatt gccgcgctgg aagaacaaat tggctatacg
    16621 ctatttaccc gcaaagataa taatatcagc ctcaataaag ccggacagga actgtatcaa
    16681 aaactgttcc cggtttatca acgactttct gcgattgata atgaaatcca taactcagga
    16741 cgtcgttaac gagaaattgt gataggcata gataatactt atccgacaat tatttttgat
    16801 cagttgatta gtctcggaga taagtacgaa ggcgtgacca tccagccagt tgagttcagc
    16861 gaaaatggcg ttatcgataa tctttttgac cgtcagttag attttattat ctccccacag
    16921 catgtatctg cacgagttca ggaactggaa aacctgacta tcagtgaact accaccgctg
    16981 cgattgggct ttctcgtttc tcgccgttat gaagggcgac aggagcaaga gctgcttcag
    17041 gagctgccct agctacaaat gcgctttcaa aaccgggcta acttcgaagc aatgattgat
    17101 gcgaatatgc gtccttgtgg gatcaatcca accattattt atcgtcccta tagttttatg
    17161 gctaaaatca gcgccgtgga gcggggacat tttctgacgg tgatcccgca ttttgcctgg
    17221 cgactggtga atccggcaac gctgaaatat tttgatgcgc cgcacaggcc gatgtatatg
    17281 caggaatatc tttattcaat cagaaatcat cgttataccg ccacgatgct tcagcatatt
    17341 gctgaagatc gtgacgggac aaaccattaa cccagcatcg atccggttgc aattaaatta
    17401 cggtgtaagt cgaagacgtg gctaagatcg tggtgaatat gtccgccgga cgcattttcc
    17461 agatatcgat gcagataatc ccgatacatg ggatgtgcac aatgatcaat ggtggtgcgg
    17521 ataacgttgt tatacggatc ctgtgcgcca aatccactga ccgcttcgct ccataaataa
    17581 cgtgccgcac gcaacatggc gacgttcata aacagatcca tgccaatgcc gaagaaaaac
    17641 gacaggcgag gggcgaagtc atcaattttc agcccggcgg agattgctgc tttgatgtac
    17701 tcaatcctat cggcgagcgt aaatgctacc tgctgcacgc agttggcacc cgcttcaccc
    17761 atgtggtaac cgctgatact gatggtatta aatcgcggca tgttgccgga acaccaggcg
    17821 atgatgtctg cgataatacg cattgacggt tttggtgggt aaatataggt gttgcggcaa
    17881 agatactctt tgaggatatc gttttgaatg gtgccggtca gtttatcgga tgtaacacct
    17941 tgctcttctg cggcgacgat ataaaacgcc agtactggta gcactgcgcc attcatggtc
    18001 atcgaaaccg acattttatc cagcgggatc tggtcgaaca ggacttttat atcttccacg
    18061 gtgtcgatag cgacgcccgc tttgccgacg tcgcccgcca cgcgtgggtt atcggagtcg
    18121 tagcctcggt gggtggccag gttacggcga taaaaagcgt tggactcttt tgctgtcgag
    18181 aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca
    18241 cgaacgtagg ggggcaaacc aggaagggta cccgtcacct ccagattatc gagatcggct
    18301 tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca
    18361 gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg cacgttagac
    18421 attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg tgagcttttt
    18481 acgtgggttt gtcgccagca aaaaaatgca ttcagacgtc gttttttgcc gcctgagatt
    18541 tgtctatttt gcgatgtgat tcaaggaata caaaaagaag tgaggataac gcctgatatg
    18601 tgctatcagg cgttatttga tggattaatc ctgacgaaga actttgtgac cataatcgag
    18661 caacgcatca gtgactttac gcatcatgcg gctttcagga gcaaagcggt gccagtagag
    18721 catccggcgt tgaaatagcc ctggcgttaa gtcaatcagt tcgccgctgg ccagctcttt
    18781 ctcgatttgc aggtgcggga tcatacagca ggtggtgccc tgacgagcaa gttgcacgaa
    18841 cgcttccgaa gaattaacga tatggcaggg tacgctgcca ggaggcagat cgaaattttg
    18901 ctgcaaaaag gcctggtgca tatcgtcaag atggtcaaac gcgaccactg gcgctttcag
    18961 taatgccgaa cgcgttacgc cgttagggaa atatttttcg gcaaatggtt ttgagctgac
    19021 gaacagatag tcgagcgcac cgagtttatc gacaagacaa ctcggcagcg cctgatgttg
    19081 aatactcact gcgccgacca cttcgccgcg acgcagacgt tcctgagtgc gggtttcatc
    19141 ttctacctgc aagttgaggc ggatgggcga atcagccaac acaggagcca gtgccggaag
    19201 caaccacgtc gccagactgt cggcgttgac cgccagtgaa agcagcagcg gagtcgaacc
    19261 ggtttgttca tcgcccagcc actcttcttc cagcagctcc acctggcgta gtagcgccaa
    19321 cagtttttgc ccttgttccg tcgggcgcgg cggtacggta cgcactaaca acggctgccc
    19381 gaacatattt tccagttgct taatgcgctg tgagacggct gattgtgtaa tgcacagctt
    19441 ttgtgccgcg cgctcaaatc ctcgttcacg tatcaccgca tccagtgcct gtaatgttct
    19501 gtagtccggg cgtttcattg ctgttgtctg ctcctggtaa atgctttttc ctgcactatg
    19561 acacaatttt gtgtcgggtt gcatataccg aagccgttgg cgggtaacat ttgtatcagt
    19621 ggttaacgcg cagcttaaaa gatcagagat ttgcgctaat gcatttcgct cagcgagttc
    19681 gcgccctggt tgtgttgaat ggcgtggcgt tattgcctca gtttgcctgt aaacaggggc
    19741 ttgcgaacgg ggaactggtg cgcctgttcg caccgtggag cggcataccc agaccgttgt
    19801 atgctttatt tgcggggcga aaagggatgc ccgccattgc gcgatatttt atggatgagt
    19861 taaccacgcg gcttgccaac ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa
    19921 tttcatacca caggcgaaac gatcatgacg caggatgaat tgaaaaaagc agtaggatgg
    19981 gcggcacttc agtatgttca gcccggcacc attgttggtg taggtacagg ttccaccgcc
    20041 gcacacttta ttgacgcact cggtacaatg aaaggccaga ttgaaggggc cgtttccagt
    20101 tcagatgctt ccactgaaaa actgaaaagc ctcggcattc acgtttttga tctcaacgaa
    20161 gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa tcaacggcca catgcaaatg
    20221 atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt tgcggaaaaa
    20281 tttatctgta ttgcagacgc ttccaagcag gttgatattc tgggcaaatt cccgctgcca
    20341 gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa actgggcggt
    20401 cgtccggaat accgtcaggg cgtagtgacc gataacggca acgtgatcct cgacgtccac
    20461 ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg
    20521 gtgactgttg gtttgtttgc taaccgtggc gcggacgttg cgctgattgg cacacctgac
    20581 ggtgtcaaaa ctattgtgaa atgatctgac gggggaacct cccccgttaa aaaaattctc
    20641 ttcattaaat ttggtgacat gtgtcacgtt tttaccaggc aattgtcgat tgctctaaat
    20701 aaatcctcta aaccagcata ttcatccaag aaatactttt gcgtgatatt tcctcaacat
    20761 cgcgacgcaa acgttcatat tgccgcaata ttattttttg atatgttgaa aggcggatgc
    20821 aaatccgcac acaacatttc aaaagacagg attgggtaaa tggcaaaggt atcgctggag
    20881 aaagacaaga ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt
    20941 cgtgcagctg gttacaccaa catcgaattt cacaaaggcg cgctggatga tgaacaatta
    21001 aaagaatcca tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca tctgactgaa
    21061 gacgtgatca acgccgcaga aaaactggtc gctattggct gtttctgtat cggaacaaac
    21121 caggttgatc tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc accgttctca
    21181 aatacgcgct ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg
    21241 ccggaagcca atgctaaagc gcatcgtggc gtgtggaaca aactggcggc gggttctttt
    21301 gaagcgcgcg gcaaaaagct gggtatcatc ggctacggtc atattggtac gcaattgggc
    21361 attctggctg aatcgctggg aatgtatgtt tacttttatg atattgaaaa caaactgccg
    21421 ctgggcaacg ccactcaggt acagcatctt tctgacctgc tgaatatgag cgatgtggtg
    21481 agtctgcatg taccagagaa tccgtcaacc aaaaatatga tgggcgcgaa agagatttcg
    21541 ctaatgaaac ccggctcgct gctgattaat gcttcgcgcg gtactgtggt ggatattcca
    21601 gcgctgtgtg acgcgctggc gagcaaacat ctggcggggg cggcaatcga cgtattcccg
    21661 acggaaccgg cgaccaatag cgatccattt acctctccgc tgtgtgaatt cgacaatgtc
    21721 cttctgacgc cacacattgg cggttcgact caggaagcgc aggagaatat tggcctggaa
    21781 gttgcgggta aattgatcaa gtattctgac aatggttcaa cgctctctgc ggtgaacttc
    21841 ccggaagtct cgctgccact gcacggtggt cgtcgtctga tgcacatcca cgaaaaccgt
    21901 ccgggtgtgc tgactgcgct caacaaaatt tttgccgagc agggcgtcaa catcgccgcg
    21961 caatatctgc aaacttccgc ccagatgggt tatgtagtta ttgatattga agccgacgaa
    22021 gacgttgccg aaaaagcgct gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt
    22081 ctgctatact aaatcccttt ctctgaaaat caacgggcag gtcactgact tgcccgttat
    22141 tttatccctt ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga
    22201 aaataaatca aagtcagatc aatgcgttgt gtttatttaa tggggattta cgatggcggg
    22261 actgtacagg gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt
    22321 atgatgcgac ttgtcatcat actgattgta ctgttactca taagtttcag cgcttattaa
    22381 cagtcagtca caggggagga gcaatccccc cttaccctta ctcactaaat taggttaaag
    22441 aatcaacgat gtcaatcagg gcgatgcagg tgtgtcgccc tcaccactcc cagactttcg
    22501 acggtgtaac caccgcaggc agtgggatat cccactcttc aacggggagt ttttccacca
    22561 actgacaatc atgcgcataa cccaccggtt gcattttata gtgctgccag ttttgtaagg
    22621 tccgatcata aaaaccaccg cccattccca ggcgctgacc gtactcatca aaggcgacca
    22681 gcggtgtaat caacacgtct aatcgggaaa ggggtagcac gtcacgcaca tccagttttg
    22741 gctcatggat cttcaacttg ttcatcacca gttcgctttg tggatggtaa ttcaggaaca
    22801 gcaaattacc ggcactaaag ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc
    22861 agagttgttc tatgagtggc tgggtgtcga gttcgccatc aaaagagagg aatacagcga
    22921 ccgtatgtgc catcaccacc gggggataag tcatcatccg ggtagcggct tgttgaccca
    22981 tttcctgctg ttgttccggc gttaacgcac gacgacgttg ccgaatcatt ttgcggattt
    23041 cttgtcggga taatgtcagt gggagttctg gtaattgcgt catggctggt gccagataag
    23101 aagggaatct ccgagatgcc gccgcaggct gtaacccttg aacccttggt tcaaggtgaa
    23161 tgtgtcgtcg cagttttaag gcttctcgga cggaccgagc atgctcacca accgcggagc
    23221 gccacattct tgtggtatga aatatcggct caggggactg gcccgcttgc gaacatctca
    23281 gagaaatttt gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc attcaaagtt
    23341 ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta tggtctgctg
    23401 cagcatccga atacgctgtt ccatacttgc cgcgtagtca cgagtcttcg ctttttcttg
    23461 cgctaactca tagctgatat tcagtgcggc aatgaagacc aactgttcag tatttgtgac
    23521 tctagtgcgt tctttcagat cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa
    23581 cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa tttggatatc
    23641 gacgggttgt gcagacatgc caccttcctg ctgattgact gcgctgcctt cgtctttaga
    23701 cccatggtct gcgaaggggc gacactatag ctaccctgat gagaagagac aagccctttt
    23761 ctggtccacc agggcccaaa gtggtagcat atcatgaata ttcctccctt tgacgacgaa
    23821 tgcttatgtc tatacagaac gaaatgcctg gttacaacga aatgaaccag tatctgaacc
    23881 aacaagggac gggtctgacc cctgctgaga tgcatggttt aatcagtggg atgatatgtg
    23941 gcggtaacga tgacagctca tggctgccgc tacttcacga cctgacgaac gaaggcatgg
    24001 ctttcggtca tgagctggca caggcactgc gcaaaatgca ctctgccacc agcgatgccc
    24061 tgcaggatga cggcttcctt tttcagcttt atctacctga tggcgatgat gtcagcgttt
    24121 tcgatcgggc tgatgcgctg gctggttggg tcaatcactt cctgcttggt cttggcgtta
    24181 cgcaaccgaa gctggacaaa gtgaccggcg aaaccggtga agccatcgac gatctgcgta
    24241 acatcgcgca gttgggttac gacgaagacg aagatcagga agagcttgaa atgtcgcttg
    24301 aagagatcat cgagtacgtc cgtgttgccg cgctgttatg ccacgacacc tttactcatc
    24361 cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag
    24421 gagagtgtta tgagtgatat atcccggcaa gaatttcagc gtcgccgtca ggccctggta
    24481 gagcaaatgc aacccggcag cgccgcgctg atttttgctg caccagaagt aacacgtagc
    24541 gccgacagcg aataccccta tcgtcagaac agtgacttct ggtacttcac cggctttaac
    24601 gaaccggaag cggtgctggt gctgattaaa agcgatgaca ctcataacca cagcgttttg
    24661 tttaaccgcg ttcgcgacct gacggcagag atctggtttg gtcgtcgctt aggccaggat
    24721 gccgcgccag agaaactggg cgttgaccgc gcactggcat tcagcgaaat caatcaacaa
    24781 ctttatcaac tacttaatgg tctggatgtg gtttaccacg cccagggcga atatgcatat
    24841 gctgatgaaa tcgtgaacag cgcgcttgag aaactgcgta aaggttcgcg gcaaaatctc
    24901 accgcaccgg caacgatgat cgactggcgt cctgttgttc atgaaatgcg cctgttcaaa
    24961 tcaccagaag agattgccgt acttcgccgc gcgggtgaaa tcaccgcact ggcgcataca
    25021 cgggcgatgg aaaaatgccg tccggggatg ttcgagtacc atctggaagg cgaaattcac
    25081 cacgaattta accgccacgg tgcgcgttac ccttcctata acaccattgt tggcagcggt
    25141 gaaaacggct gcattttgca ttacaccgaa aacgagtgtg aactgcgtga cggtgacctg
    25201 gtgttgattg acgcgggttg tgaatacaaa ggttacgctg gcgacattac ccgcaccttc
    25261 ccggtcaacg gcaaattcac tcaggctcag cgtgaaatct atgacattgt gctggagtct
    25321 cttgaaacca gcctgcgcct gtatcgtccg ggaacttcca ttcaggaagt cactggtgaa
    25381 gtggtgcgca tcatggttag cggtctggta aaactcggca tcctgaaagg tgatgttgat
    25441 gaactgatcg ctcagaacgc ccatcgtcct ttctttatgc atggccttag ccactggtta
    25501 ggactggatg ttcatgacgt tggtgtttat ggtcaggatc gctcgcgtat tctggaaccg
    25561 ggtatggtac tgaccgtaga accaggactg tatattgcgc cggatgcaga agtgccagaa
    25621 cagtatcgcg gtatcggcat tcgtattgaa gacgatattg tgattaccga aaccggtaat
    25681 gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt gatggcagct
    25741 gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc
    25801 tggctatttc ccggttaagt cacggggcgc tgccagtaca tttgattgaa gcgaccgcgc
    25861 cagagtcaca tgcgcatccg ggctttgatg gacgagctat tgcgctggcg gcgggtacct
    25921 gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc tgattgcgca actgccatca
    25981 ccaccgtgca tgttagcgat cgtggtcacg ctggatttgt caccctcgcc gcagaagatt
    26041 accaactggc ggcgctgggg caggttgtcg aattgcacaa tgtcgggcaa cggctgtttg
    26101 cattgctgcg caaagcacct ggcgtaacgc tgcattgccc tgatcgcgtg gctaacgttg
    26161 cccgtactca gagccacgtt gaagtgacgc tggagagtgg cgagacgctg acgggccgcg
    26221 tgctggtagc cgctgatggc acccattcag cgttagccac cgcgtgcggt gttgactggc
    26281 agcaggagcc ttacgaacaa ctggctgtga ttgccaacgt tgcgacttcc gttgcgcatg
    26341 aagggcgcgc ttttgaacgc tttacacaat atggcccgct ggcgatgttg ccgatgtctg
    26401 acggacgctg ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag gtgctgtcgt
    26461 ggagcgacga gaagttctgc cgtgaactcc agtcggcctt tggctggcga ctggggcaaa
    26521 ttacccacgc cggaaaacgt agtgcttatc cgctggcgtt aacccgcgcc gccaaaccta
    26581 ttacccatcg taccgtgctg gtggggaatg cggcgcaaac tctgcatccc atcgccgggc
    26641 aaggatttaa cctcggtatg cgtgatgtga tgagtcttgc agaaaccctg actcaggcgc
    26701 aggaacgcag agaagacatg ggtgattacg gcgtattgtg ccgttatcag cagcgtcgac
    26761 agcgcgatcg cgaagcaacc atcggcgtca cggacagcct tgtacatctt tttgccaacc
    26821 gttggacgcc gctggttgtc gggcgcaaca tcgggctgat gacgatggaa ttatttaccc
    26881 cggcacgcga tgtgctggcg cagcgcactc ttggttgggt ggcgcgttga aggcggtttt
    26941 aaggaataaa caatgcaaag tgttgatgta gccattgttg gcggcggcat ggtcgggctg
    27001 gcggttgcct gcggcttaca gggtatcggc ttacgcgttg ccgtactgga gcagcgcgta
    27061 ccggaacctc tggcggcgga tgcaccacca caactgcgcg tttcggctat caatgccgcc
    27121 agcgaaaaat tactcacccg tcttggcgtc tggcaggaca ttctctctcg tagggcctgt
    27181 tgctatcacg gcatggaagt gtgggacaaa gacagctttg gtcacatctc gtttgacgat
    27241 caaagcatgg gctatagcca tcttgggcat atcgttgaaa actcagtgat tcactacgcg
    27301 ctgtggaaca aagcgctgca gtcgtcagat atcactctgt tggcccccgc agaattacag
    27361 caggtcgcct ggggtgaaaa tgaaaccttc ctgacgttga aagatggcag catgttaacg
    27421 gcgcgtctgg tgattggcgc ggacggcgct aattcctggt tgcgcaacaa agccgatatt
    27481 ccgctgactt tctgggatta tcagcatcac gcgctggtgg cgaccattcg cacggaagaa
    27541 ccgcatgatg cggtggcgcg acaggttttt catggcgaag gcattctggc ctttttgccg
    27601 cttagcgatc cgcatctttg ctcgattgtc tggtcactgt cgccagagga agcgcagcgg
    27661 atgcagcagg caggcgaaga cgaatttaat cgcgcgttaa atatcgcttt tgataatcgc
    27721 ctgggcttat gcaaggttga aagtgagcgc ctggtgttcc cgctaacggg gcgttatgcg
    27781 cgccagtttg ccgcgcaccg tctggcgctg gtgggcgacg ccgcgcatac cattcacccg
    27841 ttggcggggc agggggtaaa tctcggcttt atggatgctg cagagctgat tgctgaactg
    27901 aaacggttgc atcgtcaggg taaagacatc gggcagtaca tttacctgcg tcgctatgag
    27961 cgtagccgca agcacagcgc ggcgctgatg ctggctggta tgcagggatt ccgcgatctg
    28021 ttttccgggg ctaatccggc gaaaaaattg ctgcgtgata ttggtctgaa actggctgat
    28081 acgcttcctg gcgttaagcc acaacttatt cgccaggcaa tgggattaaa cgatttgcct
    28141 gaatggctgc gttaaaaatt tctcttctgc tgtttatttg atacccatca cactttcatc
    28201 tcccggtttt ttcgccggga gattttcctc atttgaaata aactaatttc acctgcgatt
    28261 tcgcattata ttttctaatg ccacgaattt ttgatttagt gttttttgac atttttttag
    28321 ttcttaatgt tgtcttattc aaattaactt tctcatctat ttatctttat atagagattt
    28381 gttcattttt tggtttttta ttctgttacg attttggcgt tttttaacca taagctaatg
    28441 tgatgatcaa ttttacctta tggttaacag tctgtttcgg tggtaagttc aggcaaaaga
    28501 gaacgattgc gttggggacc gggagtggct ccgatgctgg gtttcgtggt gataatttcg
    28561 ccatagaaaa gttgtcagcc ccgcttattc aatgaggaca agatggcaca acagactcct
    28621 ttgtacgaac aacacacgct ttgcggcgct cgcatggtgg atttccacgg ctggatgatg
    28681 ccgctgcatt acggttcgca aatcgacgaa catcatgcgg tacgtaccga tgccggaatg
    28741 tttgatgtgt cacatatgac catcgtcgat cttcgcggca gccgcacccg ggagtttcta
    28801 cgttatctgc tggcgaacga tgtggcgaag ctcaccaaaa gcggcaaagc cctttactcg
    28861 gggatgttga atgcctctgg cggtgtgata gatgatctca tcgtctacta ctttactgaa
    28921 gatttcttcc gcctcgttgt taactccgcc acccgcgaaa aagacctctc ctggattacc
    28981 caacacgctg aacctttcgg catcgaaatt actgttcgtg atgacctttc catgatcgcc
    29041 gtacaagggc cgaatgcgca ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag
    29101 gcggtggaag ggatgaaacc gttctttggc gtgcaggcgg gcgatctgtt tattgccacc
    29161 accggttaca ccggtgaagc gggctacgaa attgcgctgc ccaatgaaaa agcggccgat
    29221 ttctggcgcg cgctggtgga agctggcgtt aagccatgtg gcctgggcgc gcgtgacacg
    29281 ctgcgtctgg aagcgggcat gaatttgtat agccaggaga tggacgaaac tatttctcct
    29341 ttagccgcca atatgggctg gactatcgcc tgggaaccgg cagatcgtga ctttatcggt
    29401 cgtgaagctc tggaagcgca gcgtgaacat ggcacagaaa aactggttgg tctggtgatg
    29461 accgaaaaag gcgtgctgcg taatgaactg ccggtacgtt ttactgatgc gcagggcaac
    29521 cagcatgaag gcattatcac cagcggtact ttctccccaa cgctgggtta tagcattgcg
    29581 ctggcgcgcg tgccggaagg tattggtgaa acggcgattg tgcaaattcg caaccgtgaa
    29641 atgccggtta aagtgacgaa acctgttttt gtgcgtaacg gcaaagccgt cgcgtgattt
    29701 acttttttgg agattgattg atgagcaacg taccagcaga actgaaatac agcaaagaac
    29761 acgaatggtt gcgtaaagaa gccgacggca cttacaccgt tggcatcacc gaacacgctc
    29821 aggagctgtt aggcgatatg gtgtttgttg acctgccgga agtgggcgca acggttagcg
    29881 cgggcgatga ctgcgcggtt gccgaatcgg taaaagcggc gtcagatatt tatgcgccag
    29941 taagcggtga aatcgtggcg gtaaacgatg cactgagcga ttctccggaa ctggtcaaca
    30001 gcgaaccgta tgcaggcggc tggattttca aaatcaaagc cagcgatgaa agcgaactgg
    30061 aatcactgct ggatgcaacc gcatacgaag cattgttaga agacgagtaa cggctttatt
    30121 cctcttctgc gggagaggat cagggtgagg aacatttacg cctcaccctc actctcttcg
    30181 taaggagaga ggttcacaat tcactgcacg tttcaggaac catcgctcat gacacagacg
    30241 ttaagccagc ttgaaaacag cggcgctttt attgaacgcc atatcggacc ggacgccgcg
    30301 caacagcaag aaatgctgaa tgccgttggc gcacaatcgt taaacgcgct gaccggccag
    30361 attgtgccga aagatattca gcttgcgact ccgccgcagg ttggcgcacc ggcgaccgaa
    30421 tacgccgcac tggcagaact caaggctatt gccagtcgca ataaacgctt cacgtcttac
    30481 atcggcatgg gttacaccgc cgtgcagcta ccgccggtta tcctgcgtaa catgctggaa
    30541 aatccgggct ggtataccgc gtatactccg tatcaacctg aagtctccca gggccgcctt
    30601 gaagcactgc tcaacttcca gcaggtaacg ctggatttaa ccgggctgga tatggcctct
    30661 gcttctcttc ttgacgaagc caccgctgcc gccgaagcaa tggcgatggc gaaacgcgtc
    30721 agcaaactga aaaatgccaa ccgcttcttt gttgcttctg acgttcatcc gcaaacgctg
    30781 gatgtggtcc gtactcgtgc cgaaaccttt ggttttgaag tgattgtcga tgatgcgcaa
    30841 aaagtgctcg accatcagga cgtcttcggc gcgctgttac agcaggtcgg cactactggt
    30901 gaaattcacg actacactgc gcttattagc gaactgaaat cacgcaaaat tgtggtcagc
    30961 gttgccgccg atattatggc gctggtgctg ttaactgcgc caggtaaaca gggcgcggat
    31021 attgtttttg gttcggcgca acgctttggc gtaccgatgg gctacggtgg cccacacgcg
    31081 gcattctttg cggcgaaaga cgaatacaaa cgctcaatgc cgggccgtat tatcggtgta
    31141 tcgaaagatg cagctggcaa caccgctctg cgcatggcga tgcagactcg tgagcaacat
    31201 attcgtcgtg aaaaagcgaa ctccaacatt tgtacatccc aggtcttgct ggcaaacatt
    31261 gccagtctgt atgccgttta ccacggtccg gttggcctga aacgtatcgc taaccgcatc
    31321 caccgtctga ccgatatcct ggcggcgggc ctgcaacaaa aaggtctgaa gctgcgccat
    31381 gcgcactatt tcgatacttt gtgtgtggaa gtggccgaca aagcgggcgt gctggcgcgt
    31441 gccgaagcgg ctgaaatcaa cctgcgtagc gatattctga acgcggttgg gatcaccctt
    31501 gatgaaacca ctacgcgcga aaacgtgatg cagcttttca gcgtgctgtt gggcgataac
    31561 cacgggctgg acatcgacac actggacaaa gacgtggctc acgacagtcg ctctattcag
    31621 gctgcgatgc tgcgcgacga cgaaatcctc acccatccgg tgtttaatcg ctaccacagc
    31681 gaaaccgaaa tgatgcgcta tatgcactcg ctggagcgta aagatctggc gctgaatcag
    31741 gcgatgatcc cgctgggttc ctgcaccatg aaactgaacg ccgccgccga gatgatcccg
    31801 atcacctggc cggaatttgc cgaactgcac ccgttctgcc cgccggaaca ggctgaaggt
    31861 tatcagcaga tgatcgcgca gctggctgag tggctggtga aactgaccgg ttacgacgcc
    31921 gtttgtatgc agccgaactc tggcgcacag ggcgaatacg cgggcctgct ggcgattcgt
    31981 cattatcatg aaagccgcaa cgaagggcat cgcgatatct gcctgatccc ggcttctgcg
    32041 cacggcacta acccggcttc cgcgcatatg gcaggaatgc aggtggtggt tgtggcgtgt
    32101 gataaaaacg gcaacatcga tctgactgat ctgcgcgcga aagccgaaca ggcgggcgat
    32161 aacctctcct gtatcatggt gacctatcct tccacccacg gcgtgtatga agaaacgatc
    32221 cgtgaagtgt gtgaagtcgt gcatcagttc ggcggtcagg tttaccttga tggcgcgaac
    32281 atgaacgctc aggttggcat cacctcgccg ggctttattg gtgcggacgt ttcccacctt
    32341 aacctgcata aaactttctg cattccgcat ggcggtggtg gtccgggtat gggaccgatc
    32401 ggtgtgaaag cgcatctggc tccgtttgta ccgggtcata gcgtggtgca aatcgaaggc
    32461 atgttaatcc gtcagggcgc ggtttctgcg gcaccgttcg gtagtgcctc tatcctgcca
    32521 atcagctgga tgtacatccg catgatgggc gcagaagggc tgaaaaaagc aagccaggtg
    32581 gcaattctta acgccaacta tattgccagc cgtctgcagg atgccttccc ggtgctgtat
    32641 accggtcgcg acggtcgcgt ggcgcacgaa tgtattctcg atattcgccc gctgaaagaa
    32701 gaaaccggca tcagcgagct ggatattgcc aagcgcctga tcgactacgg tttccacgcg
    32761 ccgaccatgt cgttcccggt ggcgggtacg ctgatggttg aaccgactga atctgaaagc
    32821 aaagtggaac tggatcgctt tatcgatgcg atgctggcta tccgcgcaga aattgaccag
    32881 gtgaaagccg gtgtctggcc gctggaagat aacccgctgg tgaacgcgcc gcacattcag
    32941 agcgaactgg tcgccgagtg ggcgcatccg tacagtcgtg aagttgcagt attcccggca
    33001 ggtgtggcag acaaatactg gccgaccgtg aaacgtctgg atgatgttta cggcgaccgt
    33061 aacctgttct gctcctgcgt accgattagc gaataccagt aatttactga ttcgactatc
    33121 ttctaaaggc gcttcggcgc ctttttcgct tgttaataaa gtgcgctttg tgtgtttcgg
    33181 ttgaggcgta atataaaata ttgcgagctc aatatattaa aagagtgcca ggcctgatga
    33241 gcggagcaca tcaggcacat cggctaatta caaattctcg ccgttgctgg caatcacctc
    33301 tttgtaccag ttaaagctct tcttacgtga acgcgacata tcgccagtac cgtcgtcatg
    33361 tttattcaca tagataaagc cgtagcgttt gctgtactgc ccggtagtga acgacacgca
    33421 gtcgatgcag ccccacggtg tgtagcccat cagatccacg ccatcgtaag tcaccgcttt
    33481 tttcatctct tcaatatggg cgcgcaggta gtcaatgcgg taatcgtcat tgatgctgcc
    33541 atcatcttcc actttgtcgt aagcgccaaa accgttttcg acaataaaca gcggcttctg
    33601 gtaacgctca tacagctcgc aaagcgcgta acgcagacca accggatcaa tctgccagcc
    33661 ccagtcagac gctttaacat acgggttcgg tacgctgcct tcaaaaccag agatcgcatc
    33721 gccggtgccg ccttcggcct tcaccgcgtt ggtcatgtaa tagctgaagc cgagataatc
    33781 gcaggtgcct tcgcgcagta catccagatc gccgtcttcc attttgatgt taaatccgcg
    33841 gcgttcccac tcgttcaaca cataggacgg gtaatagcca cgcaactgca catcggtaaa
    33901 gacgtaacgt tcgcgcatcg acttctgggc aaacatcaca tcgtccgggt tacaggagta
    33961 gggatagagc ggcaccatcg ccagcataca accgactttc atctccgggt tgatgcgatg
    34021 cgcggctttc accgccaggg cgctggcgac aaactggtga tgcagcacct gatacatggt
    34081 ctcttccggg ttttcatgtt cggtatacac cacgccggag cagcagtaac cgaacagcgg
    34141 tgcacgccag ttacgctggt tgttaatttc gttgaaggtc atccagtatt tgactttgtg
    34201 cttatagcgt tcgaatacca cttccgcgaa acggacaaag aaatcaacca ctttacggtt
    34261 ggtccagcta ccatattgct gcaccagatg cagcggcatt tcaaagtggg agagggtgat
    34321 caccggttcg atgttgtatt tcagcagttc atcgatcata tcatcgtaga acttcagccc
    34381 ttcttcgttt ggctgagctt catcgccttt tggaaaaatg cgcgtccagg cgatggaagt
    34441 acgaaaacat ttgaagccca tttcggcaaa tagcttgatg tcctccttgt agtgaccata
    34501 aaaatcaacg gcttcatggt ttggatagta tttgccaggc acaacttctt tggtgatttc
    34561 gcgcggcacg ccgtgtgcgc caccggtcag aacgtcacaa atgctcggcc ctttgccgcc
    34621 tttgttccag ccgccttcga cctgatgagc ggcaactgcg ccgccccata agaaatcttt
    34681 cggtaaggtg agttttttca ctatcatagg ctccagatga gtcaattctt acaggaaata
    34741 ttaccatgca gccaaacgac atcacttttt ttcaacgttt ccaggatgac attctggctg
    34801 ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca cttcaaaacg ggtgatgtgc
    34861 ttcgtgtcgg acgttttgaa gatgacggtt atttttgcac gattgaagtc accgcaacct
    34921 caaccgtaac gctggatacg ctgacggaaa aacatgcaga gcaggaaaat atgaccctga
    34981 ctgaactgaa aaaggtcatt gccgacatct atcccgacca gacacagttt tatgtgattg
    35041 aatttaaatg tctttaaatt gaactcacgc ctgttagttg aaatttcgca tttaatttca
    35101 tgatttttcg gagattaatc tcaaatcaga atttatttta gctaacaggt gttcactgga
    35161 actattctca gttacgctgg agaggtatac atgtgcgtgt acgtttttcc ggagtaagtt
    35221 atggttcaga agcccctcat taagcaggga tattcgctgg cagaggaaat tgccaacagc
    35281 gtcagtcacg gcattgggct ggtgtttggt atcgttgggc tggtgttgct actggttcag
    35341 gcggtggatc tcaatgccag cgccacggcg ataaccagtt acagcctcta tggcggcagt
    35401 atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca ttcctcatca acgggcaaaa
    35461 atgtggctga agaaatttga ccactgcgct atttatctgt tgattgccgg aacctacacg
    35521 ccgtttttgc tggtggggct ggattctccg ttagcgcgcg ggttgatgat tgttatctgg
    35581 agcctggcat tgctgggtat tctgtttaaa ctgaccatcg cgcaccgatt caaaatttta
    35641 tctctggtga cctatctggc gatgggctgg ctgtcgctgg tggtaattta tgaaatggca
    35701 gttaagctcg cggcgggcag cgttacctta ctggcggtag gcggtgtggt ttattcgctc
    35761 ggggtgattt tctacgtctg caagcgcatt ccatacaatc atgccatctg gcacggcttt
    35821 gtgctcggcg gtagtgtgtg ccactttctg gcgatctatt tgtatattgg gcaggtgtaa
    35881 ttggcgtgcc tttgtaggcc tgaaaagaca caccagtgtc gcatcaggcg ataaatgcct
    35941 gatgcgatac agagatttac tcttccagcg aatatggcag tggctcaata cgcaatgtat
    36001 tcgcatcgtc acgcacgcgg aagatgctgt ccggttccat atcgttattc atgacaacct
    36061 gcaccacaac ctgaccatct tccagtttga ccgcagccag caccgtaccg gtacgacgcc
    36121 agttctcgcc cattttcagc tctaagtctt caccagcttc cggcagtcgg ctggcgctac
    36181 ccgtcagcaa ccagagcgcg cgtttgttgg caccacggaa ttttgctcgc gccaccatct
    36241 cttgtccggt atagcagcct ttcttaaagc tgatgccgcc cagcgcctgg aggttggtcg
    36301 cctgggggat aaactgcccg ctgttggcgg catcaatcac cggaaaaccc gcttcaatgt
    36361 ttaatgccag ccactgttgg ctattgttca gttccgcttc accgcgcagt ttatcggtca
    36421 gcatattggc agtagcttca tcggttacga tcaggaaacg ttctgccggg tgttcaaacc
    36481 atagcaacgt ggtcgcgcct tctttgacta cctgtttttc tctcgaaggt agttcactaa
    36541 agagatttgc caacgcggtg cgcgcctgaa aaccggcaac accaagcagc acacgctcgt
    36601 cgtctggcgc gatggtcact ttagagaaca ccgcatattt tttcagttca gtcagctgcg
    36661 gttcacgcac gctgcgccgt tcaatccatg caaagccatc gccgtcgcgg aacagacgta
    36721 aattgctcca cattttacct ttggcgtcgc aatgggcggc gagcaggtgc tgatcttcag
    36781 tcatctggct gacatctgct gtcacctggc cttgcatata tttttcgctg tccgcgccag
    36841 taatagtggc aagcgcccag tcatcaagcg tcatcagcgt cagtggcaaa cgggcagaag
    36901 ccgtaggctg acggggagga aaaggtgtaa aagccataat agagtcctga gttgcttaac
    36961 ggcgtattaa tcactaatgg taaaagagct atgaggcaat gcaagtgctt tcactgcgcg
    37021 atctgtgcct tctgcgaggc gacttccaaa aaaatgaagc ccttgaattg ttgtgaatta
    37081 atttcgcgat cgcgattgtg tttactgata ttcccgtaaa agaagtcgaa aaacacgtta
    37141 caatagccgg gtactggatt ttcgtgagac aggaagaaga tggacattaa caacaaagcc
    37201 cgcattcatt gggcatgccg ccgtggtatg cgcgaactcg atatttcaat catgccgttt
    37261 ttcgaacatg agtacgacag cttaagcgat gacgaaaaac gcacctttat tcgtctgctg
    37321 gaatgtgacg atccggacct gtttaactgg ctgatgaatc acggtaaacc agccgatgca
    37381 gaactggaaa tgatggtccg actcatccag acacggaacc gggaacgtgg tcctgtggca
    37441 atctgatttg cgcgtctcct ggcgcgcaca gtggctttcc ttgctgattc atgggctggt
    37501 tgccgctgtt attttactca ttccctggcc gctcagttac accccgttat ggatggtgtt
    37561 actttcgctg gtggtgtttg attgcgttcg cagccagcgg cgcattaatg ctcgccaggg
    37621 ggaaattcgc ttgttgatgg acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt
    37681 caaagcgccg tggatgatta agagcggcat gatgctgcgt ttacgttctg acagcggaaa
    37741 acggcaacat ttatggctgg cagctgacag catggacgaa gccgaatggc gggatttacg
    37801 gcggatattg ttgcaacaag agacgcaaag ataaagatga cggcaaagca tatgtgctaa
    37861 gccgtcagtg acatcaggcg taatgctctg ccatttcgtt gaggatttgc tcgcaccagc
    37921 tttgaatacg ctcgtcgcta aggtcatact ggttagtttc atccagcgcc agacccacga
    37981 acagttgccc gtcagcaatc accggtttcg gactggtaaa ttcatatcct tccgttggcc
    38041 agtagccgac gaacttcacg cctttggtcg agagtttgtc atgcagcata ccgagcgcat
    38101 cgaggaacca ctcgccgtat cccagttgat cgccaagccc atacagcgca acaattttac
    38161 cttcgaggtt caggtcgtcg agctgatccc agacggcttc ccagtcttcc tggatttcgc
    38221 caaaatccca ggtcgggata cccagaatga gcacatcgta ctgctccatt aatttcgggg
    38281 agtcgtcctt gaggttatgt aaggtcacca gttctgggcc gataatatcg cggatttttt
    38341 ctgccgccat ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata ttcataacgt
    38401 aagcttctcg attctgctgt ggcttttgcg gctagtgtat cagaatcgct ttagtcacgg
    38461 cacaataagg cataatggat caaatgagtg accataaggg gcgcaagtga aacaggatct
    38521 ggcacgcatc gagcagtttc ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac
    38581 gttgaacgct taccgtcgcg atctgtcaat gatggtggag tggttgcatc accgcgggtt
    38641 gacgctggcg acggcgcaaa gtgacgattt gcaggcatta ctggcagaac ggctggaggg
    38701 cgggtataaa gccaccagct cagcgcgttt gctgagtgca gtgcgccgat tgttccagta
    38761 tctttatcgc gaaaagtttc gtgaagatga tcccagtgcg catctcgctt caccgaaatt
    38821 gccccagcgt ttgccaaaag atttaagcga agcgcaggtc gaacgtttat tacaggcacc
    38881 attaattgat cagccactgg agctacgcga taaagccatg cttgaagtgt tgtatgctac
    38941 cggactgcgt gtctctgaac tggtcgggct gacaatgagt gatatcagcc tgcgtcaggg
    39001 cgtggtgcgg gtcattggta aaggcaacaa agagcgcctg gtgccgttag gtgaagaggc
    39061 ggtttactgg ctggaaacct atctggaaca tgggcgtccg tggctgttga atggtgtgtc
    39121 aattgacgtg ttgtttccca gccagcgtgc gcagcagatg acgcgacaga ccttctggca
    39181 ccgcattaaa cattatgccg tgctggcggg tatcgacagc gaaaagctgt caccgcatgt
    39241 gttgcgtcac gcttttgcca ctcacttatt aaatcatggt gcggatttac gcgtggtgca
    39301 gatgctactg ggccacagcg atctctccac cacgcaaatt tatacgcatg tcgctaccga
    39361 gcgtctgcga caacttcatc aacagcatca cccgcgggcg tgatgtctga aaagaacagg
    39421 aaaatttatg aagaaaggtt ttatgttatt taccttgttg gcagcgtttt caggctttgt
    39481 tcaggctgat gacgcggcaa ttcaacaaac gttagccaaa atgggcatca aaagcagcga
    39541 tattcagccc gcgcccgtag ctggcatgaa gacagttctg actaacagcg gtgtgttgta
    39601 catcaccgat gatggtaaac atatcattca ggggccaatg tatgacgtta gtggcacggc
    39661 tccggtcaat gtcaccaata agatgctgtt aaagcagttg aatgcgcttg aaaaagagat
    39721 gatcgtttat aaagcgccgc aggaaaaaca cgtcatcacc gtgtttactg atattacctg
    39781 tggttactgc cacaaactgc atgagcaaat ggcagactat aacgcgctgg ggatcaccgt
    39841 gcgttatctt gctttcccgc gccaggggct ggacagcgat gcagagaaag aaatgaaagc
    39901 tatctggtgt gcgaaagata aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt
    39961 cgcaccagcc agctgcgacg tagatattgc cgaccattac gcacttggcg tccagcttgg
    40021 cgttagcggt actccggcag ttgtgctgag caatggcaca cttgttccgg gttaccagcc
    40081 gccgaaagac atgaaagaat tcctcgacga acaccaaaaa atgaccagcg gtaaataatt
    40141 cgcgtgaaac aacagataca acttcgtcgc cgtgaagtcg atgaaacggc cgacttgccc
    40201 gctgaattgc ctcccttgct gcgccgttta tatgccagcc ggggtgtgcg tagcgcgcag
    40261 gaactggaac gcagtgttaa aggcatgctg ccctggcagc aactgagcgg cgtcgaaaag
    40321 gccgttgaga tcctttacaa cgcttttcgc gaaggaacgc ggattattgt ggtcggtgat
    40381 ttcgacgccg acggtgccac cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt
    40441 tgcagcaata tcgactacct ggttccaaac cgtttcgaag acggttacgg cttaagcccg
    40501 gaagtagtcg atcaggctca tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt
    40561 atttcctccc atgcgggggt tgaacacgct cgttcgttgg gcattccggt tattgttacc
    40621 gatcaccatt tgccaggcga cacattaccc gcagcggaag cgatcattaa ccctaacttg
    40681 cgcgactgta atttcccgtc gaaatcactg gcgggcgttg gcgtggcgtt ttatctgatg
    40741 ctggcgctgc gcaccttttt gcgcgatcag ggctggtttg atgagcgcgg catcgcaatt
    40801 cctaatctgg cagaactgct ggatctggta gcgttgggga cagtggcgga cgtcgtgccg
    40861 ctggacgcta ataatcgcat tctgacctgg caggggatga gtcgcatccg tgccggaaag
    40921 tgccgtccgg ggattaaagc gttgctggaa gtggccaatc gcgatccaca aaaactcgcc
    40981 gccagcgatt taggttttgc gctggggcca cgtctcaatg ctgccgggcg actggacgat
    41041 atgtccgttg gcgtggcgct gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca
    41101 aatgaactcg atgcgctaaa ccagacgcga aaagagatcg aacagggaat gcaagttgaa
    41161 gccctgacct tgtgcgagaa actggagcgc agccgcgaca cgctacccgg cgggctggca
    41221 atgtatcacc ccgaatggca tcagggcgtt gtcggtattc tggcttcgcg catcaaagag
    41281 cgttttcatc gtccggttat cgcctttgcg ccagctggtg atggtacgct gaaaggttcc
    41341 ggtcgctcca ttcaggggct gcatatgcgt gatgcgctgg agcggctaga cacgctttac
    41401 cccggcatga tgctcaagtt tggcggtcat gcgatggcgg cgggtttgtc gctggaagag
    41461 gagaaattcg aactctttca acaacggttt ggcgaactgg ttactgagtg gctggaccct
    41521 tcgctattgc aaggcgaagt ggtgtcggac ggtccgttaa gtccggccga aatgaccatg
    41581 gaagtggcgc agctgttgcg cgatgctggc ccgtgggggc agatgttccc ggagccgctg
    41641 tttgatggtc atttccgtct gctgcaacag cggctggtgg gcgaacgtca tttgaaagtc
    41701 atggtcgaac cggtcggcgg cggtccactg ctggacggga ttgcttttaa tgtcgatacc
    41761 gccctctggc cggataacgg cgtgcgcgaa gtgcaactgg cttacaagct cgatatcaac
    41821 gagtttcgcg gcaaccgcag cctgcaaatt atcatcgaca atatctggcc aatttagcgt
    41881 catcttctct ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc accgcatttt
    41941 gactagctca ataaaagaaa tcagaccatg tttgaaatta atccggtaaa taatcgcatt
    42001 caggacctca cggaacgctc cgacgttctt agggggtatc tttgactacg acgccaagaa
    42061 agagcgtctg gaagaagtaa acgccgagct ggaacagccg gatgtctgga acgaacccga
    42121 acgcgcacag gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg acaccctcga
    42181 ccaaatgaaa caggggctgg aagatgtttc tggtctgctg gaactggctg tagaagctga
    42241 cgacgaagaa acctttaacg aagccgttgc tgaactcgac gccctggaag aaaaactggc
    42301 gcagcttgag ttccgccgta tgttctctgg cgaatatgac agcgccgact gctacctcga
    42361 tattcaggcg gggtctggcg gtacggaagc acaggactgg gcgagcatgc ttgagcgtat
    42421 gtatctgcgc tgggctgaat cgcgtggttt caaaactgaa atcatcgaag agtcggaagg
    42481 tgaagtggcg ggtattaaat ccgtgacgat caaaatctcc ggcgattacg cttatggctg
    42541 gctgcgtaca gaaaccggcg ttcaccgcct ggtgcgtaag agcccgtttg actccggcgg
    42601 tcgtcgccac acgtcgttca gctccgcgtt tgtttacccg gaagttgatg atgatatcga
    42661 tatcgaaatc aatccagcgg atctgcgcat cgacgtttat cgcgcatccg gcgcgggcgg
    42721 tcagcacgtt aaccgtaccg aatctgcggt gcgtattact cacatcccaa ccgggatcgt
    42781 gacccaatgc cagaacgacc gttcccagca caagaacaaa gaccaggcca tgaagcagat
    42841 gaaagcgaag ctttatgaac tggagatgca gaagaaaaat gccgagaaac aggcgatgga
    42901 agataacaaa tccgacatcg gctggggcag ccagattcgt tcttatgtcc ttgatgactc
    42961 ccgcattaaa gatctgcgta ccggggtaga aactcgcaac acgcaggccg tgctggacgg
    43021 cagcctggat caatttatcg aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc
    43081 tgaacaacac gcacagggcg ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg
    43141 tcgtgagaag ctggcgaacc tgcgtgagca ggggattgcc ttcccgaacg atttccgtcg
    43201 cgatcatacc tctgaccaat tgcacgcaga attcgacggt aaagagaacg aagaactgga
    43261 agcgctgaac atcgaagtcg ccgttgctgg ccgcatgatg acccgtcgta ttatgggtaa
    43321 agcgtctttc gttaccctgc aggacgttgg cggtcgcatt cagttgtacg ttgcccgtga
    43381 cgatctgccg gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg gcgatatcct
    43441 cggcgcgaaa ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc actgtaccga
    43501 gctgcgtctg ctgaccaaag cactgcgtcc gctgccggat aaattccacg gcttgcagga
    43561 tcaggaagcg cgctatcgtc agcgttattt ggatctcatc tctaacgatg aatcccgcaa
    43621 cacctttaaa gtgcgctcgc agatcctctc tggtattcgc cagttcatgg tgaaccgcgg
    43681 ctttatggaa gttgaaacgc cgatgatgca ggtgatccct ggcggtgccg ctgcgcgtcc
    43741 gtttatcact caccataacg cgctggatct cgacatgtac ctgcgtatcg cgccggaact
    43801 gtacctcaag cgtctggtgg tcggtggctt cgagcgtgta ttcgaaatca accgtaactt
    43861 ccgtaacgaa ggtatttccg tacgtcataa cccagagttc accatgatgg aactctatat
    43921 ggcttacgca gattacaaag atctgatcga gctgaccgaa tcgctgttcc gtactctggc
    43981 acaggatatt ctcggtaaga cggaagtgac ctacggcgac gtaacgctgg acttcggtaa
    44041 accgttcgaa aaactgacca tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat
    44101 ggcggatctg gacaacttcg actctgcgaa agcgattgct gaatctatcg gcatccacgt
    44161 tgaaaagagc tggggtctgg gccgtatcgt taccgagatc ttcgaagaag tggcagaagc
    44221 tcatctgatc cagccgacct tcattactga atatccggca gaagtttctc ctctggcgcg
    44281 tcgtaacgac gttaacccgg aaatcacaga ccgctttgag ttcttcattg gtgggcgtga
    44341 aatcggtaac ggctttagcg aactgaatga cgcggaagat caggcgcagc gcttcctgga
    44401 tcaggttgcc gcgaaagatg caggtgacga cgaagcgatg ttctacgacg aagattatgt
    44461 caccgcactg gaacatggct taccgccgac agccggtctg ggaattggta tcgaccgtat
    44521 ggtgatgctg ttcaccaaca gccataccat ccgcgacgtt attctgttcc cggcgatgcg
    44581 tccggtaaaa taagcattac gttatgctca caaccccggc aaccgtcggg gttttttatt
    44641 taagttgggt aaatgcagat aatcgttttc tggcttcgcg atttgtcgcc tgcatcacca
    44701 tccacggact gaacgcccac ggcgtagcat caataccgcg taaaaccgct gctaaatcac
    44761 accattgata atccatcact tcatcatcgt tgatctgtaa cgcactggtc gtgcgtgcgg
    44821 caaataccgg acacacttca ttttccacaa tgccattcgg atcggtggcg cggtagcgaa
    44881 agtcaggata gatagattca ggaggcgtaa tttctacgcc aagctcataa cggcaacggc
    44941 ggatcaccgc ctcttcgttg ctttctccca gttgtgggtg cccacaaacc gagttagtcc
    45001 acacgccagg ccatgctttt ttgctaaggg cgcggcgggt aactaataat tgccctttgg
    45061 cattaaacag ccaactggaa aacgcgagat gtaagagggt gtctgccgtg tgtgcggcat
    45121 acttttccag cgtacccgtg ggaactccct gtgcattcaa taaaatgacg tgttccgttt
    45181 gcataatttc tcacatgtaa ttctgatcac tcatcattat agataataga agagtgaaat
    45241 gattaaccaa gtgagcgttt atcgacaacc gcccgttttg agcggatgtc gacaggtaaa
    45301 aaccatttaa aaaagcagca tcatatattt gaagcgactt tcaatggcca ttgcaacaca
    45361 atcagaaatg acaggaatag agagagtaaa aagcaacagc agactgaata tagcgcgcat
    45421 taaaagattc atattattct ccatgctcat ttttcttcag cttgcgcatc agaagccaca
    45481 tacgtactgt cctgactttt aaatctttat cagagacgga ttccttcacc aggtcagcat
    45541 gatgctggta gccattaaag aagacgttca gcacaacggc gctaagggtt gcgagcataa
    45601 tgccgctatg cagcaacggt tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga
    45661 gcgttggcgt catgccaaca ccgagactga tcgccacaat atagaggttg taacggttag
    45721 tggtgtagtt acagcgcgac agaattcgaa tccctgtcgc cagtaccatg ccgaacatca
    45781 ccagccccgc gccgcccagc acaaattgcg gaatggaggc gaccagcacc gccattttgg
    45841 gcaccatgcc aaacaggatt aatataattc ccgaagaaat acacacccag cggctgtgga
    45901 cgcgcgtcac gctgaccagc ccgacgttct gcgaaaatga cgtatgtggg aagctgttaa
    45961 acgtgcctcc aatcattgtc ccgaccccat ctacgcgtaa cccacgaata atgtcctggg
    46021 aagagagttt gcgaccgact atttcaccca gtgccaggaa catccccatt gactcaataa
    46081 atacgatgat gagtactgcg gtcatggtca aaatggaaac gggatcgaaa atcggcatac
    46141 caaatgacat cggcgtgaca atcgcaaacc atgaagcatc atgtagcccg gataaattga
    46201 cttcattcat catccacgaa agtaaaaagc caaatacaat ccccagtaat acggcgacgt
    46261 tggacataaa tcctttcgca tagcgagtaa tgagcaagat aaaaattaag acggcaaagg
    46321 agatacctaa ataaacggga ttaccatatt gcggattccc tttacctccg gcggcccagt
    46381 caatacccac ctgaatgatg ctaagcccga tagaagtaat caccacaccg gtaaccagtg
    46441 gcgggaataa aggcatcaag cgaccgataa gtggcgctaa taatgtggtg ataaaacccg
    46501 cggcgatagt ggcaccgaat atccccagca ggccgatatc cgggttcata ccaatggcta
    46561 tcattggtgt tacagcggcg aaggtcaccg acataatcac cggcaggcgg atccccataa
    46621 agcggccgat accgatacat tgcaataatg tgacgatccc gcagcaaaag agatccgagc
    46681 taatgagcat cgcaatagct tcttttgata ggccgagtcg gtcaccaatc ataagaggaa
    46741 cagcgactgc acctgcgtac atgacaagga cgtgttgcag accgagtatg atcagctttc
    46801 ctggtgataa tatgcggtca acctcatcag ttgggcggtc ttgcccagaa gatgagggaa
    46861 gttgggaatc tatggcgctc atcagtgttt ctcctttcgc ccgccagagt aaaaagcaac
    46921 cgtttatgtt catgaaactg tctaaacaca tgctcgtgga gtgccagttt ttgcgaggaa
    46981 ggggagttag agacaggaat tgcggaggca atcacataat cgtggttaaa ccacgaaaat
    47041 gagaagagaa cctgacaatg gaattatctg taattttctc atattgatat ttcattaatc
    47101 attctggcat gtcgcactct cgcatttaat cgtttttatc tggatagcat tcttttgatc
    47161 ggcaatgttg aaataatgaa agcggcatag attgaggggc aataatttga actcagttgc
    47221 aaaattataa ttaaaacggt catgctacct aatatatgcc cgtttattgc atcgcgaaat
    47281 ttgagaagac ataactcatt aaatgaaagg gatgcaaatg aataagttta tcgctgctga
    47341 agctgcggaa tgtataggct gccatgcttg tgaaattgcc tgtgcggtgg cacataatca
    47401 agaaaactgg ccgctgagtc acagtgactt tcgaccgcgt atccacgttg tagggaaagg
    47461 ccaggctgcg aatccggtgg cctgccatca ctgcaacaat gccccttgcg ttacggcttg
    47521 tccggttaat gctctgactt tccagtccga tagcgtacaa ctggacgagc aaaaatgtat
    47581 tggttgtaaa agatgcgcaa tcgcttgccc ctttggcgtc gttgagatgg tcgatacgat
    47641 tgcacagaaa tgcgaccttt gtaaccagcg cagttccggc acgcaagcct gtattgacgt
    47701 ctgcccaaca caggcgttac ggctgatgga cgataaaggg ttgcagcaga taaaggtggc
    47761 ccgccagcgc aaaacggcag caggaaaagc gtcatcagac gctcagccat ctcgcagtgc
    47821 agcgttgctc cccgttaact cgcgtaaagg cgcagataaa atttcagcga gtgaacggaa
    47881 aacccacttt ggcgaaatct attgcgggct ggatccacaa caagcgactt atgagagtga
    47941 ccgctgtgtt tattgtgccg aaaaagctaa ctgcaactgg cattgtccgc tgcataacgc
    48001 tattccggat tacatccgtc tggtacagga aggaaagatt attgaagcgg cagaactttg
    48061 ccaccagacc agttccttac ccgaaatctg cggcagggta tgtccacagg accgtctttg
    48121 tgaaggtgca tgtactttga aagatcactc tggcgcagtc tctatcggta atctggaacg
    48181 ctacatcacc gatactgcgc tggcgatggg ctggcgtccc gatgtcagca aagttgttcc
    48241 ccgtagcgaa aaagtggcgg tgattggcgc tggacctgca ggattagggt gtgctgatat
    48301 tctggcgcgc gcgggagttc aggttgatgt ctttgatcgc catccagaaa ttggcggtat
    48361 gctgactttt ggcattcctc ctttcaaact cgataaaacg gtattaagcc agcggcgaga
    48421 gatattcacc gcaatgggaa tcgatttcca tctcaactgt gaaattggcc gcgatatctc
    48481 tttcaacgaa ttaacggcgg aatatgatgc tgttttcctc ggcgtgggga cttacgggat
    48541 gatgcgagca gatctgccgc atgaagatgc gcctggtgtc attcaggcgt taccgttcct
    48601 gaccgcccat acccgccagc tcatgggatt gccggagtct gcagagtatc cgctgacgga
    48661 tgtggaaggt aagcgagttg tggtattggg cggtggcgat acgacaatgg attgtttgcg
    48721 gacttccatc cgccttaatg ctgccagcgt gacctgtgcg tatcgtcgtg atgaagtcag
    48781 tatgccgggt tcgcgcaaag aggtggtcaa tgcccgcgag gaaggtgttg agtttcagtt
    48841 caatgttcag ccgcaatata tcgcttgtga tgaagatgga cgcttaacgg cggtgggcct
    48901 gattcgtacc gctatgggtg agccggggcc ggatggccgc cgtcgtcctc gtccggttgc
    48961 gggttcagag tttgaattgc ccgccgatgt tctcattatg gcctttggtt tccaggcaca
    49021 taccatgccg tggttgcagg gcagcggaat taaactcgat aaatggggcc tgattcaaac
    49081 cggcgacgtc gggtatttac ccacccagac gcatctgaaa aaagtctttg ctggtggtga
    49141 tgcagttcat ggcgcagatc tggttgtcac tgcaatggcc gcaggaaggc aggcggcgcg
    49201 cgatatgtta actctgtttg atacgaaggc atcgtgatga aatcgttaat tatcgttaat
    49261 ccggctgact gtattggctg ccgtacttgt gaagtggcct gtgtggtcgc tcacccttca
    49321 gaacaggagt tgaatgccga tatcttttta ccccggctga aggtacaacg gctggatagc
    49381 atcagtgcgc cggtgatgtg ccatcagtgt gaaaacgccc cttgtgttgg cgcttgtccc
    49441 gtgggggcgc tgacgatggg cgagcaggtg gtgcaggcaa attctgcccg ttgtattggc
    49501 tgtcagagtt gcgtcagtgc gtgcccgttt ggaatgatca ccattcagtc attgccgggg
    49561 gatacccggc aacaaatcgt gaaatgcgat ctctgtgaac agcgggaaga ggggccagcc
    49621 tgcgttgaat cttgcccgac acaggcgctg cagttgctga ctgaaagaga actcaggcga
    49681 gtccgccagc agcgtattgt tgccagcagt gagaatcctc tgtgaaagaa atctccgcgc
    49741 tgaaaggcgc ggagaatcta ttagattgcc caaccacccg cgtaaaacgc gaccagtgcg
    49801 gcagtaatga tcaccgtacc aatattcagc ttttgccatt cgcgcgcaaa gacgcgaccc
    49861 accaccagtg ttacaaagcc cagcataata ccggtaacga tattacaagt cagaacgatg
    49921 aacacggcac acaccaggcc agccattgcg tcaataaaat cgttgaaatc cagcttcgag
    49981 acgttgctta acatcagcaa acccacgtac atcagtgcgg gagctgtggc ataaccgggg
    50041 atcagaaacg ataacggtga taaaaacaga atcatcagga acaacacacc aaccacggtt
    50101 gcggttaaac cggttttccc cccggcggcg gttcctgccg ctgattcgat ataaaccgcc
    50161 gcgggcgctg cgcccaccag gccggagaat attgaactta ctgagtcact ggtcagggct
    50221 ttgccgccgt tgatgatctg gttgtcttta tccaacaaat tcgcctgacc ggcgacggca
    50281 cggatggtgc cagtggcgtc gaacactgcg gtcatcacca atgccagtac actcggaagt
    50341 acagttggct ggagtgcgcc cataatatcg aggctgaaaa tcagagactt accatcttcg
    50401 ccagtcaggc ttggcatcgc caccagaccg tggtacttca ccgctgggtc aaagattaag
    50461 ccgatgatcg aaattgcaat aatcaccaac aagatcccgc cgggtacgcg gcacttttcc
    50521 agaccgaaga tgaccgccag ccccagtaag ctcatcatca ccgggaagga ggtaaaggca
    50581 ccgagcgcca ctggcaagcc ttcaatcggg ttttttataa ccattcccac accgttagca
    50641 gcaatcagca gcagaaacag gccgataccg atacctgtac cgtgagcgat ccccatcggc
    50701 aaattacgta agatccaggt acgcacaccg gttacggaaa tagcggtgaa gatgacgccc
    50761 atcagaaaga ctgcgcccag tgcgacagga acgctaattt gttgcccgag taccagactg
    50821 aatgcggtaa acgccgtcaa ggaaatagcg caaccaatcg ccattggcaa attggcccat
    50881 aaccccatca gcaacgagcc gaagcccgcg accagacagg tggcaacaaa cacagctgcg
    50941 ggaggaaaac ctgctttgcc cagcattccc ggaacgacga taacggaata aaccatggcc
    51001 agaaaggtcg ttaagccagc cagtacttcc tgacgaacgg tactgccacg agcggtaatt
    51061 ttaaaataat tatccagcgc gccctgtggc tttggtgcgt ccggtgtttg taggatgtct
    51121 ccagacataa tgatgtcctc acgaggatgt taaagtttta ttattagttg cgttcgtaca
    51181 ccagacgacc atcaacgtag gtgcggtaga tcgaacggtc atcgcccaac gtcatcatca
    51241 cgaacaattt gtcgactaaa gaaacagagt tgtcatagcg cagctgctgt agcggagtgg
    51301 cagtgggttc catcaccacg aaatcagcct ctttgccagg taaaaagttg ccaatcaaat
    51361 cgtcaaggcc cagagatttc gctccgccga gcgtggccag gtaaaacgct tcatatgccg
    51421 agaggcgata gccttgtaat tgcaatacct tgtaggcttc gttcagcgtt tgcagcatgt
    51481 taaaagttgt tccggcaccg atatccgttc ccatgcccac tttgactttc ttctgccatg
    51541 cttttttcaa gttgaataag ccgctgccga ggtaaaggtt ggaggtcgga cagaaagcaa
    51601 tgctggattt ggtttcgctg agacgatccc actctttttc ttcgagatgg acgcagtgag
    51661 caaagacgca gtttttaccg gtcaggccgt actgatggta aacatcaaga taaccatcat
    51721 ggtcaggata aagcgatttc acccaggcaa tttcatcttt gttttcacag agatgggtat
    51781 gtacccatgt atccggatat tcttctttca ggcgttgcgc catcgccatc tgttcaggag
    51841 atgaggtagg ggcgaagcgt ggcgtaatcg catacaacag acgaccattt ttgtgccagc
    51901 gttcaatcag ttctttgctt tggtgatagc tgctttcggc agtgtcgagc agataatccg
    51961 gtgcgtttcg gtccatcatc actttaccgg caatcatacg catattgata tgactggcgg
    52021 cttcaaacag cgcatcaacg gattgtggat gaacagtgcc aaacaccagc gccgtggtgg
    52081 ttccgttacg taaaagctgc ttgatgaaga acgccgacat ctcgcgggcg tactctaaat
    52141 cctcataacg acgttcagta gggaaggtgt gtttattcaa ccactccagc aattgttcac
    52201 cataggcccc caccatttca ctttgcggat aatggatatg tgtatcgata aagcccggta
    52261 ctatcagttt gccgcgatag tcgcgcacgc gaatggtgtc aggaatttga tgctttccgt
    52321 cttcccattc gccaaaccat tccacttttc cctgtttaat gagtaataaa ccatcctcaa
    52381 taaaccgcag cgcagaggca atttcttccg ggttatcgac cgtacgggtg acatcaataa
    52441 aactgcctcg taccgctttt aacgtgtgtt ctcctgacat cattgactcc tttactgtta
    52501 atccatctct tccgctgagg taataccagg cagaacgttt tcctgtcggt agccaccagg
    52561 gaggataata tttaataaaa tcgcagttaa cccgccagca caaatagggt tttcaactaa
    52621 tacataaata gaggctggca atattttaaa aatttcggga tcgtaggaga taccaagccc
    52681 taaacctaaa gaagtggcaa caataagcgt ttcacggcgc tttaaaccgt tggtgatgat
    52741 gatgcgaatc cctgcgatgg caatcatgga aaacatcaac gtcattgcgc ctcccagaac
    52801 tgctgaggga atggtcgtga agaagccgcc aatcatcgga aataagccga ggataaccag
    52861 cattaccgcg atggttcgcc cgacataacg tgaagcgacg ccagtcatct gaataacccc
    52921 attattttgc gcaaacgtgg ttaatggtaa tgaaccgaca gcggaggcga taacagaaac
    52981 cagaccatct gccagcacgc cgcctttcag tcgggactga tactcttccc cctgaatggg
    53041 acggcgggaa accattgccg tggcggtgat atcgccgaca gcttccagca cgctaagcag
    53101 ataaatcgtg ccaaccacca ggaactgatg gaagctaaaa ctaaagccgt atttgaacgg
    53161 atgcgggatg gtgattaacg gcaaattgcg catactgctg aaatccacca tgcccaggca
    53221 taacgatgcg atatagccga cacatagccc aatggcgatc cctcccatgc gtagcaacgg
    53281 actgcgacag cagttaaagc cgatcaccac aatcaaaacc aataaaccaa cgccgagatg
    53341 ttcgtaattg ccgaacgtac cgctgctttt ggctgcaaat cctccaccaa aatcaataat
    53401 gccgacttta atcaggctta agccgatcat cagcaccaca atgccgctga cggtaggcgt
    53461 aataacccga cgtaaatagg gcaggataaa agaagagccg acaaccagaa atgcgccaac
    53521 gaaggagacg ccgaggagcg acgacatgat taactcttcg tgaaaaccgt cgcttttcat
    53581 gctgctgccc agcgcaatca tgaccgtaac aaatgaaaaa ttgactgact gaattgaaag
    53641 taaaccagaa ccgacgatgc cgtagcggtt tacttgtaac caggtaccaa taccagaggc
    53701 gatcatcgcc atagaaacaa gataggcagt tgtttcagcg gaaagctgta aggccgcacc
    53761 cacgattaac gcgggggtta ccatcggaac gaaaattgcc aacagatggg taatggcacc
    53821 tacgagagcc tgatgaaagg gagggcgatc ctccagttca aatataaggt cagaacctgc
    53881 atggtttata tcagacatcc cttcactcct tttaaagtaa atttgatcat ctgaattagt
    53941 aaaataaatt cccacgaatt tggggcgcag gacgccttga gaacggctta tctcattttc
    54001 gcagtcacta tttagtcatc gttcaaacag agaaacaggc cgccgaagcg gccatttatt
    54061 acttatattt tttccagcgc ggtgagtatt ttctccggtg tgaaatgcca ttcgcgtaac
    54121 cagatgccgc aggcatcgtg aattgcggta gcaatcgccg gagctgcgcc atttacaccg
    54181 atttccgaga tcgatttcgc cccgaatggg ccgactttat cgtcgctcgg caccagcaca
    54241 gcgcggaaat cacgcggaat gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc
    54301 gttaacgggt gaccttcggc gtcataaatg atctcttcgc tcatactgtg gccgatagca
    54361 cgcagggtgg caccgtagat ttgtcccaac gctaactctg gattgaccgg tgtaccgcag
    54421 tccagcaagg cgtagaattt atccaggcgg atttcacccg tacgcgtgtt gacggcaact
    54481 tcagcgaagt ttgcgccata cgggaaggcg aaatcaggtg tgatataact gccagttccc
    54541 accagtgaac caaagccggt gccggtttcg cctttatggg ctatctctcc gaaactaact
    54601 tcgcctttct tgccgcgaac gacgcccggc gttgctagtt gaacatctgc cactggctca
    54661 cccaatattt gcgcgccgtg gaacaggatt ttctcccgca gattttccgc tgccaaacgc
    54721 gcagcgttac ccgagaagca agtaccggac gaggcatatg cgcctttatc aaacagtgca
    54781 tgatcggtat caccagagat aacatgcacg tcctgcggtg ggcagtgcag cacttctgct
    54841 gccagtttgg tcaccacagt atccagacca gtaccaatat ccgcaccacc ggaatgaacg
    54901 ataaaggtgc catccgattc cagtttgatc atgcagttag cctgatcgat atccgggata
    54961 cccgatttct gcatgataat ggcgacgccg cgaccgatgt gccagtcacc attttgcggt
    55021 tttggtgaag accattggat catctcgcga ccctgacgca ggatctcttc cagtgcgcag
    55081 ctggcggcgg aaggaacgga ggtcggcgct ttaccttcac cgattgcacc gagaattttc
    55141 agctcttgcc cttcgtgtac ccggttacgt tcgataattt ccagttggtc gatctgtaac
    55201 tgttcagcca gttccgctaa tgccatggtg atagcgaagt tacctttcgg tgcgccataa
    55261 ccctgataag caccatttgg gcaaatgttg ctgtagtagg tggtgacctg aaaatcgacg
    55321 ttatcgcatg gatataacgg cagcgacagc gccggtccgt tacacggtac ggtgagtgag
    55381 tggttaccgt aaggacccgt gttggcgcgg aaatccatct tcactgccgt caggcgacca
    55441 tcttttttcg cgcccagttt gacggtgact ttcgcgacgt gacgagaggt gttagcaata
    55501 aactcttctt cacgggtgta gcggaaaagt accggacgcc cggtcacgca ggttgcccag
    55561 gcgcacactt cttccagcag gatgtcctgt ttggaaccaa aaccgccgcc aactcgctct
    55621 ttaatgacat gaactttatg ctgtttcatg cccacgaggc gcgcgacctg gcggcgtaag
    55681 tgccatggta cctgggtgga ggcgtggatc accagacgat cgccgtccat acgggtaaag
    55741 cagatatgtg tttcagtcgg gcactgctgt gcctgcgttg aattataggt tcgctcaatg
    55801 atcacatcgg catcggcaaa gcctttgtcc atatcgccaa tatgaccatg aatactggcg
    55861 gcgatatttt tgcgtgggcg agaaccgatc gggaagttga tgatcatatg ctcgccgcgc
    55921 tgggctgcat ggctgttatc atcttccaga gtatctggcg caccagcaac atacaccacc
    55981 ggttcatcgt gcacgacagg cgcatcttcc gccattgcct cgtcaatcga cattaccggc
    56041 ttaagcactt catattcaac gtcgatgagc ttcagtgctt cgagcgcaat gtcttcactt
    56101 tcggctacta ccgcagcaac gcgatcgccg acgtgacgca ttttcttgcc gaacattcgg
    56161 cggtcaagcg gtgacggttc cggtgcgctc tgaccacccg gggtatagta aatatccggg
    56221 caattcaggt gagtaataac gtgaacgacg cccggtaagg cttcagcttt gctgacatcc
    56281 agatgagtaa tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca
    56341 gccgttacgc gatcttcaac atagcagggt ttcgcctgca ccattttcgc ggcatcagtt
    56401 ttaggataat gcttgccaat gacttcaagg tcgtcacgga aagtcggagc gatatcaatg
    56461 gtggcctgcg gattattttt acgtgcaacc gccagttcaa tgacctgata gtattgctgc
    56521 cagccagcat cgcggctaaa cagacctgag agtgcgtcgt cgatctcttc gcgggtaggt
    56581 gcgtcgatgc gatcgagaag atcggtgata atcagagccg cagctggatc gttataacca
    56641 gactgcacca cgccaacatc aaccatggcc tgttgaacca gacttaactc attccatttg
    56701 cccagagatt ctgcggtacg aatatctgcc ttctctaact gtgcggcgat aagcaaggac
    56761 gcgttaacga tattaccgtt aaagataatt gcgtcagaac cggcaaaccc aaagccatca
    56821 tcactgttgc gtacagagtg cattcccatg ttaaacaaca gcttttgcac gttttcgcct
    56881 ggattaacgg ttagctcctg aggcgcgcca tttaaagtaa agtggatgat catacggctt
    56941 cctccccagc ctgttggcag tcggcataca ggtcggctac cagaactccc gtgatatagc
    57001 gtttataggc cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct
    57061 gttccagtgc attgccttcc agattttgag tttcaacatc atgcagacgc agtgctttac
    57121 tggcgacgcc atccagcgca attcgcatac cgtcgtggtc tgttattgca acggctgccg
    57181 tcacgacggt taaacctgcc tgagaacggc taattttgcg agtcgcacag gtgcgatacg
    57241 gatctttaat gataatttcg gttaacaggc gatcgcatgg gcaggccagg tagtcctcga
    57301 ttgacagcgt ttcgccgttg ccaaaaacca gttcagcatc cagtgccagc aggacgggaa
    57361 gcagcactga ctcttcttgc cgggcggcaa tttcaccacc aatggtcgac tgattacgaa
    57421 catggcgaga gtagacaaaa ccgagagctt cccgcagtgc tgcaggaata aatcgcgcat
    57481 cacgcagtgg ctgcaagcga gacattgcgc caattcgtag tgcaccgtta tcccagtcaa
    57541 tccagtccag ctccagatcc tgcaaggaaa tggcaatctt tttatcggta cgggttggtg
    57601 tagcgttgag tttgctgccc ccggcgaacc agacggcttc atcctggtag cggcgcttca
    57661 gttccagcgc ctgttcgaca gagtcgggtc tgaaaaattg ttcaatcatc gtctttcctt
    57721 aatttcccct cttctctgaa agaagagggg agctgttatt tatgccagcg catccatccg
    57781 acgccacata ctggcagcgg cttttctggc ttgcgcataa atggaatcgc aatcgaagtt
    57841 aaactgacgg tcttcataga ccatcacacc attgaccatc acgctgtgaa cgctacctga
    57901 gcccataccg aaagcgatat gcccggcgat attgtctgcc agcagcggcg tcggcgagtt
    57961 gtaatcgcaa atggttaaat cggctttgta accggcttcc agaagcccaa atttcgcgcc
    58021 aaagttgcgg ctcatcagtt cgttgccgtt agccagtgct ttggcaaaac tgtcaggcca
    58081 cagcgggcca cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc
    58141 cgaaccaatg ccgtccgttc ccaacgccag attgcggatg tcgctaaggt gatggttgta
    58201 gccgacatgg ttgttcatgt ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt
    58261 gagtaggacg atgtcatctt tcgacaggta cagcccatga gcgaccagcg ttttgctgtc
    58321 gatgagatcg aattgcgcaa gtcgtgccag caggtctttg ccgtaccaat ggtgactgta
    58381 ggaaacgtcg taaaggtctt ccgcaacgtg aatatgcaaa ccacgacctg tgcttttcac
    58441 ggcttcacgc agcatctcca gaccggcatc cggcacggta aacggcgcgt gagcgccgat
    58501 atgtgcttcc accagatacg gctcggttgc tgctttcttc gcctcatcaa tctgacgggt
    58561 gaagcggatg ttttcttcta caccttcctg caactctttg atgccgttgt tacggtcagt
    58621 agtttcaaaa caggtcatcg cgcgcaggcc aacttttaaa aatgcgttgc gcaatgtgga
    58681 gagcgacccg tcgatatacg ccggagaggc atggtgatcg ataaccgatg tacatccgct
    58741 cttaatcgct tccagggaac aaatcagtcc gctgtaatag agcgactctt catcaagagc
    58801 gcgatcgagc cgccaccaga gatttttcag cgttgagatg aaatccgggc agggggcgat
    58861 gtttgccata attccgcggg aaagccccga gtaaaaatgg ttgtgcgagc agacaattcc
    58921 cggcatcaca atccgaccat gcatctcttt gtagctggca tcggggtagc gttgcgtcag
    58981 ggcatcgccg atagcgacaa tcacatcatt ttcgatggcg atatcaacgc cttcctgcac
    59041 tttcgccggg tgtaactgca ctgcggtgac attcttcaga atcaacatga ttacacctcc
    59101 acgcggccca gcagataatg atgatgctgg tggacatggc taatgatgcg gcacatatcg
    59161 ttcagctccg gtggtacgtt gttgaactga ccttcgctgt cgatgtttaa cacccagctt
    59221 tggttattca gacgtacgcg tacccggcaa tcttccacaa ggaagcctgg gttactgctg
    59281 ttatcaaagt cttgcgacag gctgaagacg gtgattttgt ctttgtacgg tttaccgttc
    59341 cacgggcaga attgggcgca gttgccgcat tcgttgcagt aggcatcgag atgcagcgtc
    59401 tggaaacggt tctggaagcc aggaaccgca atggatacgt tggcgcggtt cgggcagaca
    59461 tccacacact tgctgcaaac gtagttacat tcaaggcagc gtgcggcttc ctgtgcgaca
    59521 aacgcgtcac gatcgtcact gttcaccaga gtgacagaga tatcgccttt acgttgatag
    59581 atttccgccg gatttacgtt gttccagtat ttatcgttct ggtgggaacg gatattttcc
    59641 cgactaagga tggtatcggt cgcccgacgc gcggttccga cagcagcgac aatggaggat
    59701 ggtccgcgct gtacgtcgcc gatcataaag acgtcagtca gacgcgtttc gccattatgg
    59761 tcgacgtctg gccagccgtt tttgtccagc ggcacgccca tcgcattcag ggcttcagta
    59821 tcctgctgtt caccaatggc ggtgatcaga ctgtccacat gcagtgttac tgtttcgttg
    59881 gtttcaaccg gacggcgacg acctttctca tcaggttcgc caagcgacat aacgcgcaag
    59941 gttaaggtgc catcagcatc gaaacgttcc ggattattca gaaaacggaa ctccacgccg
    60001 tcgtgcaacg cttcttcata ctcttcgcgc catgcgggca tttcttgcag tgaacgacgg
    60061 taaacgaccg ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt
    60121 gcggtgttac ccgccccgac aacgaccaca tgtttgccca gcttgagcgc ggtgcccttg
    60181 ttgtattcac gcaggaaagg gagtgatttc cagacatttt ggttgtcgcc cgccagtttc
    60241 acaccgctat ttttatcagt gccggtggca atcagaacat agtggaagcc ctgatttttt
    60301 aactgttcaa cggtcaaatc gggtgagcaa ccgtactcaa atttcacgcc gtgagcggca
    60361 acaaaatcga tatcgtgctg aattaactct gcaggaatac ggaactgagg aatgatattt
    60421 ttcaccacgc cgcccgcatt ggcttcgcgt tcaaacagcg tgaccggatg gcccgctctg
    60481 gcaaggaagt aacctgcggc cagacccgcc ggacctgcac caatcacggc taccggatgg
    60541 cgtgaaccgg aaccggctgg tttgtgccag cgttgcttat attcatccca acctttttcc
    60601 agggcgactt ttttcagttc gcggatattc agcgcactgt cgtaatccag gcgggtacag
    60661 ttgtattggc actggtgatc gcaaatatga ccggtaatgg cgggcagggc gttgcgttga
    60721 tagatgagtt ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat gtattccgga
    60781 atatcttgct tgatagcgca ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc
    60841 aggtcttcag ccacttcaat acgctcttct ggcttccagt gtttctgggt gtattccatg
    60901 gttaacgcgt ctgctgccag tctgttcagt cgttcgacgt caacgtggtt aagctcccag
    60961 gcgtcggagc cttccagctc gcgcatgcag gcacttaagc gcagatagcc gccaggtttc
    61021 agcaggtcgg ttgccatagt aatagggcga ataccagtat caaaaatatc gcggatagtc
    61081 agctgactgg caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga
    61141 actgctgcaa cgttgatgga gagcgggaac agcgcacggc ctgacatata catctcttca
    61201 ccgggcagtg cgcctttatt gttgatggtg ccgagagtgt tagtcagttt tacgccaaag
    61261 ccgagtgatt tttcttttgc cagtgccatc aggcgttcca gcatttccag tgcttgcgtc
    61321 agcttgaggt cgtgatcaaa tgactcttct tttaagccga tgtaaccgaa accgcagaca
    61381 tcgaggatct cacgaacacg cgcgtacccc agtaaggtcg ggttgagttt cacaaaggtg
    61441 ttgagtcctt tttcttccag catgtagcgg caaatggctt cgatttcatg cggaggacag
    61501 ccgtgcatgg tggagagggt aacaccttgt accatgctgg tggggatgcg agcgggtaag
    61561 gcttgcaagc tttcgcgttt ttcctgcaat ccgtggcggg ctaaaaatgc ctcatcctgg
    61621 agtagcttat taagtgcatc gcggtattga gcgaatttcg gatggtcaga tgcgtccatc
    61681 atattgtcga tgaactgctg catcggcggt tgcttaatac cttcgaggtt gtaaccgacg
    61741 ctcatattaa agatgaacga tttaccggaa tcagaaggct ggaacatcgc ttcgagaagg
    61801 tgcagggcaa accaggcttt gaggtattca tcccaggctt taagcagggt aaattcggta
    61861 gaccattcgg tgttaaagca ctcgtcttcg gcatcgatac agggcttttc cagctccaga
    61921 cggtcaagaa tttggacggt ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg
    61981 acaatatttt gcgcgagttg cgtgtgcggg ccagcggcag ggccgacggg agtggcacag
    62041 gtttcaccga agacgctaac agttttactt tttacgggtg agtaaaactg ttgctcggga
    62101 ataccaaaga ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca
    62161 aacggaatgg gacgcataat atcccccata accctctcct tagcttatac agatatcatt
    62221 gagtgaccgg tgttgagaaa tccggtggtt tagggggata gagcaacaat cgcaccactc
    62281 ttgccaggtt tgacgggaaa tataaaaaaa tgtgaggcgg ctcgaaactt aatggctttt
    62341 tgtgaatgaa ctcacaggtg ccaaatgaga attaaaatca ctcctgaaaa ttattattgc
    62401 cagcccattt atcacttttt ctcttttctc aaaatcagtg attccttatc attatgataa
    62461 agagtgctgg agggattaaa tatttgaacg caatcgttta actacgtttt ctaaatttca
    62521 gtataccttt tcttcgctgt aataaagtcg tccggagtat caatatctaa aattatttct
    62581 gcattttcta tttcgacgga ataatgttct cccataagga gtgcctgacg catattggtg
    62641 acattggggc gctggattgc ctgcatcagg catggttttg acactaaaat cggatggccg
    62701 gggatgccat tatggagtgg cagtattgcg ccatcatttc gtaacgacca gatttttcta
    62761 aaaatatcga tggtgagggt tggcatatct ccgtgggtga gaaaacaatg ttctgtttgc
    62821 accgcgggta ctgcggcctt tactgatgtc agtaaaccct gcgcataatc tgggttgtgg
    62881 ataatagtga tattgctctg gttcgcataa cgttcgtgca gttcattacc acgatagccg
    62941 gtgactaaaa taattcggct acaaaactgc aacgcatttt tgatacttgt atcaagaatt
    63001 gttccctgtt gccagggtaa catcattttc cattgcccca ttcttgatga taatcctgcg
    63061 gcggtaatta tacagtcgat ggctgacata gtgattccat tatttacagg agctaataac
    63121 gcctgataat tgcgttactt cctgatggat tacgttgagg gtaatttatc ccagagaggt
    63181 ccataaagac gcgtgaaaag tataattgac ccatcggcat tattcattga tttaggcgcg
    63241 cagaaacgtc ccactgtgat ttctattgtt ggtgctgggg ggaaaaccag cttgcttttt
    63301 tggctggcag aactgttcca ggcgagtggc aggcgtgtat taatcactac gacgacacat
    63361 atgtttatgc caacatctca ctggcccgtg gttttctgtc gtgatcccgc catgcttcct
    63421 catgcgtctt ttatatcccc catttcattt tgttttcact gctggaaagc gaaccaggga
    63481 aaagtgcagg gatttacgcc agaagcgatt gatgcactgg tacaacgacc agagtgtgac
    63541 gtaattctca ttgaggcaga tggctcgcgt ggaatgccgt taaaagcgcc tgatgagcac
    63601 gaaccttgca tacctaaaag cagttgctgc gtgattgctg tgatgggagg acatattttg
    63661 ggtgcgaaag tgagcacaga aaatgtccat cgctggtcgc agtttgctga tattactggg
    63721 ttaacacctg atgcaccctt gcaactgagc gatctcgttg cgctggttcg ccaccctcag
    63781 ggcgcgttta aaaacgtacc gcaaggttgt cggcgagtct ggttcattaa ccgtttttct
    63841 caatgtgaga atgcgattgc gcaaagcgag ctccttcaac cgctgcaaca acacaacgta
    63901 gaggcaatct ggctgggcga tatacaagaa catcctgcaa tcgcgcgcag atttgtgaat
    63961 tagcggcccg ggtattccgg gttactgaaa ggatatccat tgaggttggt atgaatattt
    64021 tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt tgcgatggcg caaattgttg
    64081 atagccgagg ctcaaccccc cgccattctg cacaaatgtt agtgcgcgcc gatggttcta
    64141 tcgtcggtac aattggtggc ggaatggttg agcggaaggt gattgaagag tcgcttcagg
    64201 cattgcagga acgtaagccg cgattattcc atggacgtat ggctcgtaac ggtgcggatg
    64261 ctgtcgggtc agattgtgga ggtgcaatgt cagtatttat cagcgtccat ggtatgcgtc
    64321 cacgtctggt gttgattggt gcggggcatg tcaaccgggc gatagcccag agtgcggcgc
    64381 tattaggatt tgatatcgcc gttgccgata tttatcgcga aagcctcaat cctgaactat
    64441 tcccaccatc aaccacgctt ctccatgctg agtcgtttgg tgcggcagtg gaagcactgg
    64501 atattcgccc tgataatttt gtcctgattg ccacgaataa tcaggatcgt gaagccctcg
    64561 acaaactcat tgaacaaccc attgcatggc tggggttgct ggcaagtcgc cgcaaggttc
    64621 agcttttcct acaccaattg cgtgagaaag gcgtggctga agaacatatt gcccgtttac
    64681 atgcgcccgt tggttacaac ataggtgcgg aaacgccgca ggagatcgcc atcagcgtgc
    64741 tggcagaaat attacaggtg aaaaataacg cgccgggtgg gctgatgatg aagccttctc
    64801 atccttccgg acaccagctg gtggtgattc gcggtgcggg ggatatcgcc agtggtgtag
    64861 cgctacgtct gtatcatgcg ggttttaaag tgatcatgtt ggaagtggaa aaaccgacag
    64921 tgattcgttg taccgtggcg tttgcccagg ccgtgttcga tggcgaaatg acggtcgaag
    64981 gcgtcaccgc ccgcctggca accagctctg cggaagcgat gaaacttacc gaacgcggat
    65041 ttatccctgt gatggtagat cccacctgtt cattgcttga tgaactgaaa ccgctttgcg
    65101 tggtggacgc tattctggcg aaacagaatt tgggaacgcg ggcagatatg gcaccagtaa
    65161 caatcgcgct tgggccgggc tttactgcag ggaaggattg tcatgcggta attgaaacaa
    65221 atcgcgggca ctggctcggt caggtgattt actcaggttg tgcgcaggag aataccggtg
    65281 ttcccggcaa tattatgggg cataccaccc gacgagtgat ccgcgctcct gctgcaggca
    65341 ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga gggcgatgtc attgcctgga
    65401 ttggtgagca tgaaattaaa gcaccgttga cggggatggt gcgtggcttg ttgaacgacg
    65461 gactggccgt ggtcggtggt tttaaaattg gtgatatcga tcctcgtggt gaaacggctg
    65521 atttcaccag cgtttctgat aaagcccggg cgattggcgg cggcgtactt gaggcgttaa
    65581 tgatgttgat gcatcagggc gttaaagcga caaaagaagt gctggaagtg gcttaaatga
    65641 aaatggccgg atggtattac ccatccggcc tttaaattac tgacaaatca ccgttccggt
    65701 tttgccttca atcccttctt tcgctttgct caatacggta atcagtgctt cacgacctgc
    65761 gcgagagcga gcaaacgagg cggcagcttc cactttaggc agcatagaac ctttcgcaaa
    65821 atgaccttct tcgataaagc gttcagcatc acttaatgac aggcgatcga gccactgttc
    65881 attctctttt ccgaagttaa tggccacttt ttctaccgca gtcagaatga tcagcatatc
    65941 ggcatcgatc atttctgcta aacgggcact ggcccagtct ttatcgataa ccgcgctggc
    66001 accacgcaga tggttacctt cacgaataac agggatacca ccaccgccaa cggtgatcac
    66061 cacctgtccg gcatctacca gagctttaac cgtttctttt tcaatgatat caaccggttt
    66121 tggcgaagca acgacacgac gatagccgcg acccgcatct tctttcagag tgtaaccctg
    66181 ttttgtcagt agctctgctt cttgctcggt aaagaacgaa ccaatcggct tggtcgggtt
    66241 gaggaatgcc ggatcgttag catcgacttc gacctgggta accagcgttg ctacaggttt
    66301 attaatgcca cgagaaagca gttcttctct taaggcgttt tgtagatcgt aaccaatata
    66361 accctggctt aaggcaacac agacagacat cggcagcatt ggcgagtgcg cttcagtttt
    66421 tgcggcagct tcaaaggcct ggttaatcat gcccacctgt ggcccgttgc catgagtgac
    66481 gatgacctca tgtccctggg caattaaatc aacaattgcc tgagaagtaa tttttaccgc
    66541 tttcatttgt ccggccaggt catcgcccag cgcattcccg cccagggcga gaacaatttt
    66601 cttactcatt tttcctcact catcattctt gattgtgtat agatccctgg cagtggttag
    66661 agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac ctcgcccggc tttgcctgta
    66721 aaggtgccgt cgcagaaaat cgtttcacca cgggataagg ttctgacaat cgcgccctga
    66781 caggtaaaac cctcccatgg cgagtagtca gcgttgtcgt ggagatggcg atgctgaatt
    66841 tgttggctct gacgtgggtc tataatcacc acgtcgccat cggaaccggg cgctaataat
    66901 cctttttgtg gccacaggcc aaacagcctg gcgggcattg cactggttaa ctcaacaaaa
    66961 cgctccggtg agatacgccc cgtcatcacg ccactggaga acagcagttg catgcggttc
    67021 tccacaccgg gtaagccatt tgggcagcgg ctgaaatcgc ctttagaaat ttgcaggcgt
    67081 tgagccatcg agaaggtgca gtgatcggtt gccaccacgt caatcgcacc atcgctgatg
    67141 ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg gtgggctaag aatgaacttc
    67201 atgccatctt ctgtatcgta actgcgttcg tccaacagga gatattgtgg gcaggtttca
    67261 acccagactg gctggtgatt cgcacgggca agacgcagat aatccagacc taagccgtta
    67321 gacaggtgca cgatatagag cggggcgtta ccggcaattt gtgccaggtt aatcatgcgg
    67381 gcgatggctt ccgcttcgca ttccagaggt cgactcaagg catgataacg cggcgcggtt
    67441 aaccctgcgg cgataaactc cgcccgcttg ctggcgatag ctgcgtcatt ttccgggtgc
    67501 acggtggtca gcgcgccgga ctcatgcaag cggcgtaatg cctgcaacac ttcatcatcg
    67561 ttgagtttat attgataggt taaatagagt ttaaaactgc tcagtccttc ctcaaccatc
    67621 atcggaattt cgtcgaggat agcgtgatta atatgttgaa tgacaccgtg aaagctgtag
    67681 tcgatgactg ctttgtgggc ggcataaccg cgataaactt ccagttgatg gcgtaaccgg
    67741 cagccatttg ggccaaatcc catatggtca ataatggttg ttgtaccgcc acacgcagct
    67801 gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga tgccgacatc aatattgaaa
    67861 tgcgtatgga catccacgcc gccagggaaa acgtaacagc cagaggcatc aatttcttcg
    67921 caagggagtt gcggcgaaat atcggtaccc aactggcgaa caatcccgct ttcaatcagc
    67981 aaatcctgct tcgcctgtcc atctgcatta acaacaatgc cgttttttat caatacgcgc
    68041 atagcaaact ccaaaaaccc tccggtgaac cggaggggaa taagtgaaga gtaattattc
    68101 ggttgccagc cagcttaatg ggattgcagc gtacatagcg gcacaggtta ccaggtgaga
    68161 tttccaggtt ttttcgtttg gcgcgtgcgc ttccggttct ttaccggggc caaaaccgat
    68221 gaccggaatg ccgtggcgac ccatgatgga tacgccatta gtcgagaagg tccacttatc
    68281 aacgacaggc gctttaccaa acagcccttc ataagcattc accagtgctt taacggtgaa
    68341 gtgatcttct tccactttcc aggtcgggaa gtagcattcg gttgggtaaa ccaggccagt
    68401 ccaggacggg cggtcgtagt tgtacataga aacgacagca ttcgcttttt gtactgcagg
    68461 cagggcacgg atctcgtcca gcgcgccttc ccaggtttcg ccccaggtca gacgacggtc
    68521 gattgaaact gcgcagctgt ctgcgacagc gcaacggctt ggggaggtga agaaaatttc
    68581 agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa cccagacgtt gggagagttc
    68641 ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg ttatcaccgc gttctggtgc
    68701 cgaaccgtgg cagctaacac cctgaacatc aatacgaatt tccatacgac cgcgttgacc
    68761 acggtatacc tggcagtcgg ttggttcggt actgaccaca aattccgggc gaatgccgga
    68821 ttgttcaata atgtactgcc agcacaggcc gtcgcagtct tcttcctgca cagtaccggt
    68881 aaccagcagg gtatattcat cttccagacc gagatcttta atgattttac cggcatatac
    68941 catagacgcc atgccgcctt cctggtcgga agtaccacga ccaccgatca gctcgtcggt
    69001 ttccatgcct tcatacggat cgaagtccca gtttttgatg ttgccaatgc cgactgtatc
    69061 gatatgagcg tccattgcca ccagacgcgg gccgtggccg atataaccga gaacgttgcc
    69121 cattgggtcg atttcaactt tatcgaagcc gactttttcc atctcttctt taatacgatg
    69181 tactacgcgt ttctcgtcgc agctttcact gggaatagca accatatcgc gcaggaagcg
    69241 agtcatgtct gcctggtaat cttttgcttt ttcaagaatc agtttgaatg gaatattctt
    69301 agccattatt tttctccaga ttttttactc cccgttttgc agggagcagt tccaataggg
    69361 tgattaaggt gctacagcgt gtttgccttc ccagacaact tcgcggtagt gcttcacgtc
    69421 ggtgtcgcct tcagtgctga taaccagcac cacggcatct ttgttcagcg ccagtttttc
    69481 catcaggctt tgacgttgcg ggtgataatg aaccgctgcg agaacgccca aaccgacagc
    69541 gccggattca ccggagatga tgcgcgggtc gttgccgtac ggattaccca gcacgcgcat
    69601 acctaatgcg gcaacgctgt cctggcagga gatgaattgg gtggcgcagt tacgtaggat
    69661 ttcccagccc agcgggttag gttcgccgca ggccaggccc gccatgatgg tggccatatc
    69721 accgccaacg ttgacgatgt cgcctttgac gccggagcga taaatacagt cagctttgtc
    69781 aggttcaaca ataatgctgt gcagattttg cgggctatag acgtcgacca gataacccag
    69841 cacaccaccg gccattgcac cgacacctgc ttgcagcaga acgtgtgtcg gggttacgcc
    69901 catttcacgc atttgctcga cggcttcatc cgccagtgtt gcgtagcctt gcatgatcca
    69961 ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt accacttccc agccgtgctg
    70021 ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag ttcatatccg tgacgatgca
    70081 ctcggcaccg aggtttagaa tggcgtcaac gcgttcctga gcagaacctt tcggcatgta
    70141 aatcaccgca ttctgtccga gttgctgagc ggcccatgcc acaccgcgcc cgtggttgcc
    70201 gtcggtggtg gtcgcgaaag tcattttttc gccaatggca tttttcaggt gctcaaatga
    70261 tagcgtttcg atatcaagat gatatttttc acacaataat tgagcgatgg cgtatgcgcc
    70321 gccgagcatt ttgaaggcgt tcagaccgaa gcgttttgat tcgtccttaa cgagaatttt
    70381 ctttacacca aacaggtttg cgagatcgtc cagcgcacaa agcggtgttg ggcgataacc
    70441 agcaattttc tggtggaact ggcgtgccag ttttgcctgg ctttgcgaaa aaagcggtga
    70501 tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc tttaatgaga aaacggacat
    70561 atcctatcct tttggcctct ggaaatagag ggccgaccag ctgatcggcc cgaggggtta
    70621 tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc agtgcacctg gtttggcgta
    70681 tttacgggac aggatcatcg cggcgatgat ataaggcttc cagctggctt ctttgtaggt
    70741 agcgatacgg tatttttcga atacgccttc agtcacttca ccttctttgc aggatacgcc
    70801 gctgatatca gctggcaggc agtgcatgta cagggcttcg ccatcacggg tcagctccat
    70861 catctcttcg gtacaatgcc agtctttgtg ctgtgcgttc tgtgccagac actgtttttc
    70921 cagcgctttc agtccttcgt gatcgttcgc acgcagcagt tcagtacgct cttccatcac
    70981 tttgtaaggt gcccaggact tcggataaac gatgtctgcg tctttgaagg cttcttccat
    71041 gctggtgacc tgacggaagc taccaccgga ggctttagca ttgtttttag caacttcaac
    71101 gacatccggg atcaggtcgt agccttccgg atgggccagg gtgacatcca taccgaagcg
    71161 ggtcatcaga ccgatgatgc cttgtggtac agagagcggt ttgccatagc ttggggagta
    71221 ggcccaggtc atggcgattt ttttaccttt caggttttcc agtgaaccaa agtgttcacg
    71281 taaccatgcc agatcagcca ttgattgagt cgggtggtca atatcgcatt gcaggttcac
    71341 taaagccgga cgctgcggca gcacaccctg cttgtaaccg tcatcaagtg cagcgccaac
    71401 ttcacgcata taggcgttgc ctgcgcccag atacatatcg tcgcgaatac cgatagcgtc
    71461 agcgcagaag gagatcatat tggcggtttc acgcacggtt tcgccgtgag cgatttgtga
    71521 tttaccttcg tcaagatctt gttgtgcaag gccgagcagg ttaagcgcgg aagcataaga
    71581 gaagcgggta cgggtggaat tgtcgcggaa tacggaaata cctaatccac tattaaagac
    71641 tttggttgag atgttttcag cacgcagtgc ttttaacgcg gcggcaacgt ccagtacttg
    71701 tttcagttcg tctggcgtct gttcccacgt taacaaaaaa tctttttcat gcaggtgaga
    71761 ggtcagcgaa ttgatatcct taatcagctc attaacagtt ttcatcataa agtcccgata
    71821 gtggaatgac aggcaaagtg gtttatttca ctgtgacgtg ccaaagtgat tggtgttttc
    71881 acagctatac ctcggactaa caataagtgt gccagaatta tccacgcggc tgcgaaaggc
    71941 taatttgacg gataatgtgt ggggttgatc acatattgat gccagtttcc ggttgtttgt
    72001 aaaatacgaa aacgtttggc tttttttatt acgttattta tttgctgcta agtatcagaa
    72061 ataagataaa gaggcaggct atctctcttc agtttgataa acttgccgct gtaattctca
    72121 tattgatata gaaatgtgat atctcttcca taattttatt agaaagtgat aaaaatctta
    72181 tgtttttatc aaagccaatt tcgcctgagt taatatcatc atgatctgtg aaccatcaac
    72241 gtcttcaatg atcgactttt aagtaagtct ggaaggtaaa gctatggagc ttgcgactac
    72301 gcagtcagta ttgatgcaaa ttcaaccaac aattcagcgt tttgccagaa tgcttgccag
    72361 cgttttgcag cttgaggttg agatcgttga tgaaaacttg tgtcgcgtcg ccggaacggg
    72421 cgcgtatggg aagtttcttg gccgccagtt gagcggcaac tcacgtctgc tccgccacgt
    72481 cctggaaacg aaaactgaaa aagttgtgac acagtctcgc ttcgatcccc tttgtgaagg
    72541 ttgcgatagt aaagaaaatt gccgcgaaaa agcatttctg ggtacgccag tcattttaca
    72601 ggatcgttgt gttggggtga taagtttgat tgccgttacc cacgagcaac aagaacatat
    72661 cagtgataat ttacgcgaat tttccgatta cgttcgccat atatccacca tttttgtttc
    72721 taaacttctg gaggatcagg gaccaggaga taacatcagt aaaatattcg cgaccatgat
    72781 cgataatatg gatcagggcg tgttagtggt tgatgctgat aatcgggttc agtttgttaa
    72841 tcagacagct ttaaaaacac tcggtgttgt acaaaataat attattggaa aacctgttcg
    72901 tttcagacca ttaacatttg agagtaattt tacccatggt catatgcaac atattgtttc
    72961 gtgggatgat aaaagtgaat taatcattgg tcagttgcat aacatccagg gccgacaact
    73021 atttttaatg gcgtttcacc aatcgcatac cagtttttct gtagcaaatg cacctgatga
    73081 accgcatatt gaacaattgg ttggcgagtg ccgtgttatg cggcaattaa aacgactcat
    73141 tagccgtatt gcacccagcc catccagcgt tatggtggtt ggtgaaagcg gcacgggtaa
    73201 agaagtcgtc gcccgtgcaa tccataagtt gagcggaaga cggaataaac cctttattgc
    73261 tatcaactgt gccgcgattc cggaacagct tctggagagc gaactgttcg gttatgttaa
    73321 aggcgcattt actggcgctt ctgccaacgg taaaacaggg ttgattcagg cggcgaatac
    73381 gggcacgctg tttctcgatg aaattggtga tatgccatta atgttgcagg ctaaattact
    73441 gcgcgctatt gaggcgcgtg aaattctgcc gattggtgcc agtagcccaa tacaagtcga
    73501 catccgcatc atttctgcaa ctaatcagaa tttggcccag ttcattgccg aaggtaaatt
    73561 ccgcgaagat ctcttctacc gacttaatgt tatcccgata actcttccac cgctgcgtga
    73621 acgtcaggaa gatattgaat tactagtgca ttacttttta catctgcata cccgtcgtct
    73681 gggatcggtt tatcctggca ttgctcctga tgtcgtcgaa atattgcgta agcatcgttg
    73741 gcccggaaac ctgcgcgagt taagcaattt gatggaatat ctggttaacg ttgttccttc
    73801 aggtgaagtt atcgacagca cgctattgcc gccaaatctg ctgaataatg gcacaacgga
    73861 gcaaagtgat gtaacagagg ccagtgaggc gcacctgtca ctcgatgatg cgggcggcac
    73921 ggcgctggaa gagatggaaa agcaaatgat ccgcgaggcg ctttcacgtc ataacagcaa
    73981 gaaggaagtt gctgatgaac tgggcatcgg cattgctacg ctctatcgca agattaagaa
    74041 atatgagttg ttaaacacat aacctctgag aatccggcct gaattcaggc cggatatctt
    74101 ttactttgtt ttctcgcaat ccagaactgt atttacaatc atctgatacc ccgtgcagcg
    74161 acaaagattt cccgccagtc cacgacgaat ttccgtaatg gttaatggct tctcgcgtgg
    74221 tttcgccagc attgccgtgg tagccataat caggcccggc gtacaaaacc cgcattgcac
    74281 tgcaccagat ttcgcataag ccagttggac atgagaaagt ttaccgcctt tcgcttcacc
    74341 ttccagcgtg cggatctctt ttccttcagc ccaggccgca aggaataagc aactgtctat
    74401 cgcagtgccg tcgaccagca ccgtacaggc accacattca cccacgcagc acccttgttt
    74461 gacacttagc agtccttgtt cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg
    74521 aagctgaaaa ggcatcccgt taatggtgca ttcgatggta attgtttcgc tgtgattcat
    74581 tgcaatttcc cccccgccgc ggcgacggct tcgctaatca cttttttggt catcgtctgg
    74641 atgagatgca gacgaaactc tttactggcc cgccatgacg aacgcggggc gacatcttgc
    74701 aggactgatt cgctgatggc ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc
    74761 tgttcggcat gttggcagcg aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat
    74821 tcgctgaaat tgccgttatc cagtcggcaa tgtgcggcgc agccaattgt tgaaatatcc
    74881 attgcgtcgc gcatggcata tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc
    74941 ggaaaatgaa aggcgacaag gatttcgtca tgctcaagag acactttgcc cggcccggtg
    75001 tgaaagccat taatcgggac gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg
    75061 tcataaatta gcgttggcgt ggcagaatct gcgctggtgg caccgttgca aatatttccg
    75121 ccgtaggtgg cgacattacg gatctgcggc ccggcgattg atgcagccgc agcacataac
    75181 gccgggagat gacgttgagt tacaggatct tcaatgagct gggtaaatgt cgttgcagag
    75241 ccgatacgta gcgcgccatc ttccgccagc gtaattcccc gcagctccgc cagattatga
    75301 atatcaacaa tatggcgata acggtcattg tgatggtgaa gctgtatcag tacgtcagtg
    75361 ccaccggcga gcagtttggc ctgcgggttg tcagtcagca ggttgatggc atcggcaagg
    75421 gttgctgcgc gatggtaaga agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc
    75481 cagatggaat tcttcatata atcgttttgg cgttagcggc agtgtattga ttgcaacacc
    75541 ggtagccatc ttcaccgcgt tacgaatagc agcggctaca ggaattatgg gtggctcacc
    75601 cagtgactta tgtccgtatg cggattgcgg ctcattgatt tcgacgaacg cgctttccag
    75661 ttgtggcaga tccggcatgg tcggcatttt gtaatccagc agattggggt tacggaccac
    75721 gccgcttttc gcatcgatga tcatctcttc aaatagcgcc cagccaatgc ccatccccat
    75781 tccgccgtgt acctgacctt ctgccagcag cggattaaga atatggcccg aatcatgaac
    75841 gttgaggatg cggttgatgg ttactttaca cagtgcaata tcgaccgtca gatcaacaaa
    75901 ggtacagcca aacgccggtg ggttagtggt ggttttgatg gagctttcgg cagagagctg
    75961 cccgccgcgt tcagggtggt agaaagcgtc catcgccaaa tcttttaacg acattaacgg
    76021 ctcttccggt cgttcaacca gcacgatatg gccttttatc agggtcagat tcatcgctga
    76081 ctgatgtagc atgactgcgg cgtgagcgat gattttctct tttaataaca gtgccgcact
    76141 gcgcagcgca ggcgcggcaa catagctctg acgtgaggca aatgcgccgg ggtcgaatgg
    76201 tgtaacgtcg gtgtcttgtg tcgaaattac gcggacatcg ctgaccggaa ctcccacggt
    76261 ttctgccacc atttgcgaga acacggtgtc ggcaccctgg ccgatttccg tcgcgccgct
    76321 ttgcacgttg atggttccat cctgattcat caacaggcgc gcgcctgcta tttctacgcc
    76381 gacaggccag gtgttagagg tgtagctaaa acaggcgacg ccaacgccac gacgtaaatt
    76441 gccttgctgg ttctggcact ctgcacggcg tttttcccat tcaaagattt tccggccttt
    76501 ttcaagacac tccggcaacc ctgcgctgta aatacgtttt cccgtgagcg gattagcatc
    76561 tccttcgcgg gcggcgttgc gtaaacgaat ttcaacagga tcaataccta acgctgtcgc
    76621 ggcatcatca agcatagact caacggcaaa tacgacttgt ggcgcgccat aaccacgcat
    76681 cgcaccagcc gaggggaggt tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg
    76741 aggataaagg taagcgactt tattcccccc agcagaagca atggagtgcc cgtgagatgc
    76801 ataagcgccg gtgttagaca gaacatccag actataacct ttcaatgttc cgtcgcggtt
    76861 cacgcccatt tgcccgtcaa tagtaaaagc gtggcgggta cgggttgcga ggaaacactc
    76921 ttcacggcta agagaaactt tcaccggaat gccgccaagt ttgcttgtca ggaatgccgc
    76981 cattggctct tccagtacat cctgtttatt accaaaaccg ccaccgataa acggtttgat
    77041 gactcgtacg catgaccagg gaatatccag cgcctgacca accacgcggc gaacaatgtg
    77101 cgggatctgg gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc atgccagcga
    77161 tgtcacgctt tccatatgac aatgttgaat aacgggcgtc tgatagtgcc cctgtacctg
    77221 gtagtcggcg gcatcgattg tttgttggac attacccgtc gacatcgtgc tttgtttcag
    77281 taaattgcca ccgttatgga ttggtgcagc gtcttctgcc agcgccgctt ctgacgaggt
    77341 gataacgggt aattcttgcc actcaatgct gaccaattgc accgcttttt ctgccgtgag
    77401 ttcatcgcgg gctacgacga tggcaacggc gtcaccatga tgacgaacat gacgcgttag
    77461 cagggcacga tcggcagtat cgcgcttgtt ttcgtcaagt gtccaggcat gccctgccgt
    77521 ggcgaatggg atttctggca catcttccca ggtaaaaatc gccaggacgc ccggcaaact
    77581 cctggcttgt tcatcattaa tacttacggc ataaccatgt gcgatagggc tacgtacata
    77641 tttcgcgtaa cacatgcccg ccataacata atcgtcagta tatcgtgccc gcccggtgac
    77701 cttagcaatg gcatcgacgc gcatgcatga ttcacccgta gcggttgctt cccgcgcttc
    77761 catagaaatc ccctcggttt gaacatttgg ttcttatggc aaagatttag tcttagcagg
    77821 caagaattac gccagaaacg actatatgcg tgaggggagg aaggaagtgt gatgacgtta
    77881 aaaaaacaca gatatgatac gaaacaggac tctgaatagg gacagtgata cgtatcaata
    77941 tgagtggcga taaaatcaat gtgataaatt aggattatta cgcatcaagg tgggaaatag
    78001 ggtaaaagtc aggaaattcg ataccactac agcaaatatt ttgctgatga tacttccctt
    78061 tgccaaatga aattaacctc tttctctatt acatcgtttt tccagaagca agagattggg
    78121 tatcaagagg ctggctgcta tgatatggcg tcttgttttt taagcgagga aagattttga
    78181 gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat gttgttatcg gttggactgc
    78241 ttttggcggg ctgttcgggt agcaaatcat ccgatacagg aacgtattcc ggctccgttt
    78301 acaccgtgaa acggggggat acgctatatc gtatttcgcg caccacggga accagcgtaa
    78361 aagaactggc gcgactgaac ggcatttccc ccccttacac cattgaagta ggtcagaaac
    78421 taaaactggg tggggcgaaa agtagcagta gtacacgtaa atcaaccgcc aaatccacga
    78481 ccaaaaccgc atcggttaca ccgtcatcag cggtaccgaa atcttcctgg ccgccagtag
    78541 ggcaacgttg ttggttatgg ccaacgacag ggaaagttat catgccgtat tcgacagcag
    78601 atggcggcaa taaagggatt gatatctcag ctccacgagg tacacctatt tacgccgcgg
    78661 gtgcaggaaa ggtggtgtat gtgggcaacc agctgcgtgg ctacggtaat ctcatcatga
    78721 ttaaacacag tgaagattac attacggctt acgctcataa tgacacgatg ctggtaaata
    78781 atgggcaaag cgtgaaggct gggcaaaaaa tcgccactat ggggagcact gatgcggcat
    78841 ctgttcgcct gcatttccag attcgttacc gtgcaacggc aattgatccg ctacgttact
    78901 tgccgcctca gggcagcaag ccaaaatgct gatggcgaat taatcagcag tcagcagcgt
    78961 ggtacttgct aaggagagcg taaggtttat aatgccttac gcatctcgaa gcgggcgtag
    79021 ttcaatggta gaacgagagc ttcccaagct ctatacgagg gttcgattcc cttcgcccgc
    79081 tccaatttat cttcacatca tctacctgcc atttttgttg tatctgattt tctatattat
    79141 cttaattcaa gcgcttcaga cttttcgcaa tcgccgtaat acttccccat ttttttgtaa
    79201 ataaaaagcg tttacgcaat gcaatcgtca tgtatcttaa atagtcttat ttaagatgtt
    79261 cagaactctt tgtgtgttta actggctgat aattatataa tattacgaga gaagtgatag
    79321 agcggaataa taacaaatga acattatgaa aactgggatg gttcaaaatt gaagcaattt
    79381 tgaaccatcc acaacggata ttagctaaag aacattaccg atacgcacag gatacccacg
    79441 atcagggtaa tcagatttcc gatggaacgc cagggtttaa gcgccgggat gagatacgtt
    79501 gacagcgtag gcatgatgaa aagtatcatg gcaatgagcg ggccgctgat cgcgtaaatc
    79561 atcgaaatcg cgttcgggtt aatgcaacaa acaatgaagg taatcagcga taccaacata
    79621 attgatagtg cgcggttaaa tgcacgactt ttctttacac caacctgctg taatgttgtt
    79681 ttgacgacct ctgtggcacc ttcaataacg ccaaagtacg tacccaggaa tgatttcgac
    79741 atcgcaacta ctgcgacaat aatcccggaa attgacaacc atgctggggc gttcggcagc
    79801 atagaaagcg ccgataaaat ggtaacccct tcttctttag ccgcttcaat atacgaaggt
    79861 ggaatagaaa gcaggcagct aaagacaaag aacaatacac ttatgcagat gatgagataa
    79921 gcgactttca taattttttt gcatttatcc atagcatgtt cgccatattt ttcacgtctg
    79981 tctatggcaa acgtagaaat aatgggcgta tggctaaaag cgaaaaccat cacgggaatc
    80041 gatatccata tctggtgaag ggtattctga ttgaactcta cctgggttgt taatagatca
    80101 ggttgccaac taccgaccag gtaaatagaa agaaataaga aataggcaat caatgggaat
    80161 accaaaaatc ccattacccg aatagtggca tggcgtccca tcagaaaaat gagattcagg
    80221 attaatacaa cccccagact cactagcata cggatgcgaa gatcaataac catatgcttt
    80281 gccagctgtt ccgtaagtga gttggtaatt gccactgcat atatcaacac gacgacaaaa
    80341 aaggcgatga agtacagcgt ggtaatcaga ttaccaatct tcttgccata gtagtgtgtt
    80401 accgcgcccg ttatcccttc acctgctgat gttttcgatg agaggatgaa ctggcataag
    80461 gctttatgtg gccaatatgt taaaggccag gcgaccagag cagtaataaa caggaccaca
    80521 gcccccgccg aacctaactg aataggaagg aagagcgtgc ctgcaccaac ggctgtgccg
    80581 tagagtgcga aactccacag agtttcttct tttgaccaaa tattagacat tatctgaacg
    80641 tatcatctac aaattaaaca aaatggaggg caatttacca taaaccaggt taagtatggg
    80701 agattaacgc cttatctacc tctctattgg tgggttagtg gttgcaaacc ttacgtgcca
    80761 gttatgtctc tgactactac tcaggcgtat ttcctttgtg ataatggtca ctttttttaa
    80821 acaattaaga taactgtaat taacaatcat ttactattgc actgttaatt ggtgcgaggt
    80881 tgtgatgaaa gacgttgtga ttgtcggggc gttacggaca cctatcggct gctttcgtgg
    80941 tgcgttagcg ggtcattccg ccgtggaact tggcagcctg gtcgtcaaag cgttaataga
    81001 acgtaccggc gttcctgcat atgcggtgga tgaagtgatt cttggtcagg tgttgactgc
    81061 aggggcaggg cagaatccgg caagacaatc ggctattaaa ggtggtcttc ctaatagcgt
    81121 ttctgcaatc actattaatg acgtttgtgg ttccgggctt aaagcactgc atctggctac
    81181 tcaggcgata cagtgtggcg aggctgatat tgtcatcgcc ggtggccagg aaaacatgag
    81241 ccgcgcacca catgttctga ctgatagccg caccggcgca cagcttggca atagccagtt
    81301 ggttgatagt cttgtgcatg atgggttgtg ggatgccttc aatgattatc atattggtgt
    81361 caccgccgaa aatctggctc gcgaatatgg catcagccgt cagttgcagg atgcttacgc
    81421 acttagctcg caacaaaaag cgcgagcggc gattgacgcc ggacgattta aagatgagat
    81481 cgtcccggta ataacccaaa gtaacggtca gacgttggtt gttgataccg atgaacagcc
    81541 acgcactgac accagcgcag aaggcttagc ccgtttaaat ccttcatttg atagtctcgg
    81601 ttctgtgaca gcgggtaatg catcatccat aaacgatggg gcagctgcag taatgatgat
    81661 gagcgaagcc aaagcacgag cgttgaattt acccgtgctg gcccgcatcc gcgcatttgc
    81721 cagcgttggt gtggatccgg cattgatggg aattgcgccg gtgtatgcga cccgccgttg
    81781 cctggagcgt gttggctggc agttggctga tgtcgatctt atcgaggcta atgaagcgtt
    81841 tgctgcacag gcgctttcgg ttggcaagat gcttgaatgg gatgagcgtc gggtcaatgt
    81901 taatggtggt gcgatcgcac tcggtcaccc gataggcgct tccggttgcc gaatcctggt
    81961 ttctcttgtt catgaaatgg tgaaacgtaa tgcccgcaaa ggactggcaa cgctttgtat
    82021 cggcggaggc cagggtgtgg cattgaccat tgaacgtgac gagtagctct gcattacctc
    82081 cttaccgata aatatctgcc gtcattaatc tgttgaataa tgatggctgg tgagtcgaca
    82141 taccttttca gggctccctg atttttccat tctcatgaca tattcgtgat cgacactgca
    82201 ctttttttaa tccttaactt gaaaatgaaa cattgtttta tttttaattg aaaaccctat
    82261 cccatgggca tatcatcccc tcatagagaa aacgaaacag tgttttatta ttgttttcta
    82321 ttttcacgct tcactgatta tcggaggttg atgtggacgt aagacagagc atccacagcg
    82381 cgcacgcaaa aacgctggat acccaggggc tgcgcaatga atttttggtt gaaaaggtat
    82441 ttgtcgccga tgagtacacc atggtttaca gccacattga ccgtattatt gttggcggca
    82501 ttatgccggt aactaaaacg gtttccgttg gcggggaagt tggtaaacaa ctcggcgtaa
    82561 gctatttcct tgaacgtcgc gagttaggtg ttatcaatat tggcggtgcc ggtacgatta
    82621 ctgtcgatgg tcaatgctat gaaatcggtc accgcgacgc cctgtatgtt ggtaaaggtg
    82681 caaaagaagt tgtctttgcc agtattgata ccgccactcc ggcgaagttt tattacaatt
    82741 gcgcacccgc gcatacgacg tatcccacca aaaaagtcac accggacgaa gtatctccag
    82801 tcacgttagg cgataacctc accagtaacc gtcgcacgat taacaaatat tttgtcccgg
    82861 atgtgctgga aacctgccaa ttgagtatgg ggctgacgga gctggctcca ggtaacttgt
    82921 ggaacaccat gccgtgtcac acccacgagc gccggatgga agtttatttc tatttcaata
    82981 tggatgatga cgcctgcgtt ttccacatga tggggcaacc gcaagaaacg cgtcacattg
    83041 tgatgcataa cgagcaggcg gtgatctccc cgagctggtc gatccattcc ggcgtcggaa
    83101 ccaaagctta tacctttatc tggggcatgg tcggtgaaaa ccaggttttt gatgatatgg
    83161 accatgtggc cgttaaagat ttgcgctagt tgtgggcata aacgaataag gtattgttat
    83221 gattttaagt gcattttctc tcgaaggtaa agttgcggtc gtcactggtt gtgatactgg
    83281 gctgggccag gggatggcgt tggggctggc gcaagcgggc tgtgacattg ttggcattaa
    83341 catcgttgaa ccgactgaaa ccatcaagca ggtcacggcg ctggggcgtc gttttttaag
    83401 cctgaccgcc gatctgcgaa agattgatgg cattccagca ctgctggatc gcgcggtagc
    83461 ggagtttggt catattgata tcctggtgaa taacgccgga ttgattcgcc gcgaagacgc
    83521 tctcgaattc agcgaaaaag actgggacga tgtcatgaac ctgaatatca agagcgtatt
    83581 cttcatgtct caggcagcgg cgaaacactt tatcgcgcaa ggcaatggcg gcaagattat
    83641 caatatcgcg tcaatgctct ccttccaggg cgggatccgt gtgccttctt ataccgcatc
    83701 aaaaagcggt gtgatgggcg tgacgcgatt gatggcgaac gaatgggcta aacacaacat
    83761 taatgttaat gcgatagctc cgggttacat ggcgaccaac aatactcaac aactgcgggc
    83821 agatgaacaa cgtagcgcgg aaattctcga ccgcattcca gctggccgtt ggggactgcc
    83881 gagtgacctg atggggccgg tggtgttcct tgcctccagc gcttcagatt atgtgaatgg
    83941 ttataccatt gccgtggatg gcggttggct ggcgcgttaa ttcattcctc ttacttttat
    84001 gaccctgtcg catggcaggg ttttttatac ctgtagatca tcataatcca tatcatggtt
    84061 atgaaataat ccatatcaat tatcaattga tgaactttat gaattttatc tgctttaaaa
    84121 ttaggtggtt aataataatc tcaataattc aacttaattt gaaaattgga atatccatca
    84181 cataacgaca tgtcgtagca atttaatcca tatttatgct atttccgacc tgacacctgt
    84241 gtgaattgtt cacgtatttt ttcactatgt cttactctct gctggcagga aaaaatggtt
    84301 actatcaata cggaatctgc tttaacgcca cgtcctctgc gggatacgcg gcgtatgaat
    84361 atgtttgttt cggtagctgc tgcggtcgca ggattgttat ttggtcttga tatcggcgta
    84421 atcgccggag cgttgccgtt cattaccgat cactttgtgc tgaccagtcg tttgcaggaa
    84481 tgggtggtca gtagcatgat gctcggtgca gcaattggtg cgctgtttaa tggttggctg
    84541 tcgttccgcc tggggcgtaa atacagcctg atggcggggg ccatcctgtt tgtactcggt
    84601 tcgatagggt ccgcttttgc gaccagcgta gagatgttaa tcgccgctcg tgtggtgttg
    84661 ggcattgctg tcgggatcgc gtcttacacc gctcctctat atctttctga aatggcaagt
    84721 gaaaacgttc gcggtaagat gatcagcatg taccagttga tggtcactct cggcatcgtg
    84781 ctggcgtttt tatccgatac agcgttcagt tatagcggta actggcgcgc aatgttgggg
    84841 gttcttgctt taccagcagt tctgctgatt attctggtgg tcttcctgcc aaatagcccg
    84901 cgctggctgg cggaaaaggg gcgtcatatt gaggcggaag aagtattgcg tatgctgcgc
    84961 gatacgtcgg aaaaagcgcg agaagaactc aacgaaattc gcgaaagcct gaagttaaaa
    85021 cagggcggtt gggcactgtt taagatcaac cgtaacgtcc gtcgtgctgt atttctcggt
    85081 atgttgttgc aggcgatgca gcagtttacc ggtatgaaca tcatcatgta ctacgcgcca
    85141 cgtatcttca aaatggcggg ctttacgacc acagaacaac agatgattgc gactctggtc
    85201 gtggggctga cctttatgtt cgctaccttc attgcggtct ttacggtaga taaagcaggg
    85261 cgtaagccgg cgctgaaaat tggtttcagc gtgatggcgt taggcactct ggtgctgggt
    85321 tactgcctga tgcagtttga taacggtacg gcttccagtg gcttgtcctg gctctctgtt
    85381 ggcatgacga tgatgtgtat tgccggttat gcgatgagcg ccgcgccagt ggtgtggatc
    85441 ttgtgctctg aaattcagcc gctgaaatgc cgcgattttg gcatcacctg ctcaacgacg
    85501 acaaactggg tgtcgaatat gattatcggc gcgaccttcc tgacactgct tgatagcatt
    85561 ggcgctgccg gtacgttctg gctctacact gcgctgaaca ttgcgtttgt gggcatcact
    85621 ttctggctca ttccggaaac caaaaatgtc acgctggaac atatcgagcg caaactgatg
    85681 gcaggcgaaa agttgagaaa tatcggcgtc tga
//
LOCUS       DAOFSV010000004        82658 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.004, whole genome shotgun sequence.
ACCESSION   DAOFSV010000004 DAOFSV010000000
VERSION     DAOFSV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.004"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            22..807
                     /locus_tag="QBH27_000285"
     CDS             22..807
                     /locus_tag="QBH27_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7012660.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            877..2331
                     /locus_tag="QBH27_000286"
     CDS             877..2331
                     /locus_tag="QBH27_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDQ7012661.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKKVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            2353..3762
                     /locus_tag="QBH27_000287"
     CDS             2353..3762
                     /locus_tag="QBH27_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7012662.1"
                     /translation="MTVRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            3740..4519
                     /locus_tag="QBH27_000288"
     CDS             3740..4519
                     /locus_tag="QBH27_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDQ7012663.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIGGQTVAEKAQKLWQDYLRQRMQP"
     gene            4516..5376
                     /locus_tag="QBH27_000289"
     CDS             4516..5376
                     /locus_tag="QBH27_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDQ7012664.1"
                     /translation="MNIVIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMVAEQV
                     LDALVEQWQRTPADVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLPSGMQQLNIVPGVLPDWLVSIENLKNV
                     IRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(5524..6099)
                     /locus_tag="QBH27_000290"
     CDS             complement(5524..6099)
                     /locus_tag="QBH27_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDQ7012665.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(6116..6376)
                     /locus_tag="QBH27_000291"
     CDS             complement(6116..6376)
                     /locus_tag="QBH27_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDQ7012666.1"
                     /translation="MSVARNLWRAADAPHIVPTDSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(6367..7638)
                     /locus_tag="QBH27_000292"
     CDS             complement(6367..7638)
                     /locus_tag="QBH27_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ7012667.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSSVPASELLTRFKTHPAVRPLIKNTELLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            complement(7716..8078)
                     /gene="queD"
                     /locus_tag="QBH27_000293"
     CDS             complement(7716..8078)
                     /gene="queD"
                     /locus_tag="QBH27_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDQ7012668.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8397..10196
                     /gene="cysJ"
                     /locus_tag="QBH27_000294"
     CDS             8397..10196
                     /gene="cysJ"
                     /locus_tag="QBH27_000294"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDQ7012669.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSDLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            10196..11908
                     /gene="cysI"
                     /locus_tag="QBH27_000295"
     CDS             10196..11908
                     /gene="cysI"
                     /locus_tag="QBH27_000295"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDQ7012670.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            11982..12716
                     /gene="cysH"
                     /locus_tag="QBH27_000296"
     CDS             11982..12716
                     /gene="cysH"
                     /locus_tag="QBH27_000296"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ7012671.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            12982..13134
                     /locus_tag="QBH27_000297"
     CDS             12982..13134
                     /locus_tag="QBH27_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDQ7012672.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(13260..14297)
                     /gene="iap"
                     /locus_tag="QBH27_000298"
     CDS             complement(13260..14297)
                     /gene="iap"
                     /locus_tag="QBH27_000298"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDQ7012673.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKTPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14549..15457
                     /gene="cysD"
                     /locus_tag="QBH27_000299"
     CDS             14549..15457
                     /gene="cysD"
                     /locus_tag="QBH27_000299"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDQ7012674.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15459..16886
                     /gene="cysN"
                     /locus_tag="QBH27_000300"
     CDS             15459..16886
                     /gene="cysN"
                     /locus_tag="QBH27_000300"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDQ7012675.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEVLPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16886..17491
                     /gene="cysC"
                     /locus_tag="QBH27_000301"
     CDS             16886..17491
                     /gene="cysC"
                     /locus_tag="QBH27_000301"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDQ7012676.1"
                     /translation="MALHDENVVWHSHPVTLQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17540..17863
                     /locus_tag="QBH27_000302"
     CDS             17540..17863
                     /locus_tag="QBH27_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDQ7012677.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            18056..18367
                     /gene="ftsB"
                     /locus_tag="QBH27_000303"
     CDS             18056..18367
                     /gene="ftsB"
                     /locus_tag="QBH27_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDQ7012678.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18386..19096
                     /gene="ispD"
                     /locus_tag="QBH27_000304"
     CDS             18386..19096
                     /gene="ispD"
                     /locus_tag="QBH27_000304"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDQ7012679.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            19096..19575
                     /gene="ispF"
                     /locus_tag="QBH27_000305"
     CDS             19096..19575
                     /gene="ispF"
                     /locus_tag="QBH27_000305"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDQ7012680.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19572..20621
                     /gene="truD"
                     /locus_tag="QBH27_000306"
     CDS             19572..20621
                     /gene="truD"
                     /locus_tag="QBH27_000306"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDQ7012681.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALLGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20602..21363
                     /gene="surE"
                     /locus_tag="QBH27_000307"
     CDS             20602..21363
                     /gene="surE"
                     /locus_tag="QBH27_000307"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDQ7012682.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21357..21983
                     /gene="pcm"
                     /locus_tag="QBH27_000308"
     CDS             21357..21983
                     /gene="pcm"
                     /locus_tag="QBH27_000308"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDQ7012683.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            22123..23262
                     /gene="nlpD"
                     /locus_tag="QBH27_000309"
     CDS             22123..23262
                     /gene="nlpD"
                     /locus_tag="QBH27_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDQ7012684.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTVPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23325..24317
                     /gene="rpoS"
                     /locus_tag="QBH27_000310"
     CDS             23325..24317
                     /gene="rpoS"
                     /locus_tag="QBH27_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDQ7012685.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(24488..25039)
                     /locus_tag="QBH27_000311"
     CDS             complement(24488..25039)
                     /locus_tag="QBH27_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDQ7012686.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGAEQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            25264..26307
                     /locus_tag="QBH27_000312"
     CDS             25264..26307
                     /locus_tag="QBH27_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDQ7012687.1"
                     /translation="MQGIKIYTILASSLISGSVFAQTEISTSNSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
     gene            complement(26450..27814)
                     /locus_tag="QBH27_000313"
     CDS             complement(26450..27814)
                     /locus_tag="QBH27_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDQ7012688.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(27903..28679)
                     /locus_tag="QBH27_000314"
     CDS             complement(27903..28679)
                     /locus_tag="QBH27_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDQ7012689.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRNYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTKEGLGWFDAWR"
     gene            complement(28684..29322)
                     /locus_tag="QBH27_000315"
     CDS             complement(28684..29322)
                     /locus_tag="QBH27_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDQ7012690.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDNPSKEVLFHLALYRNNPLCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(29319..30581)
                     /locus_tag="QBH27_000316"
     CDS             complement(29319..30581)
                     /locus_tag="QBH27_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDQ7012691.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(30578..31486)
                     /locus_tag="QBH27_000317"
     CDS             complement(30578..31486)
                     /locus_tag="QBH27_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ7012692.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IANADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            31682..32449
                     /locus_tag="QBH27_000318"
     CDS             31682..32449
                     /locus_tag="QBH27_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7012693.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(32500..33156)
                     /gene="pphB"
                     /locus_tag="QBH27_000319"
     CDS             complement(32500..33156)
                     /gene="pphB"
                     /locus_tag="QBH27_000319"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDQ7012694.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHLLPHIIEITNDNIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(33262..35823)
                     /gene="mutS"
                     /locus_tag="QBH27_000320"
     CDS             complement(33262..35823)
                     /gene="mutS"
                     /locus_tag="QBH27_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDQ7012695.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            36109..36453
                     /locus_tag="QBH27_000321"
     CDS             36109..36453
                     /locus_tag="QBH27_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDQ7012696.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(36492..36920)
                     /locus_tag="QBH27_000322"
     CDS             complement(36492..36920)
                     /locus_tag="QBH27_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDQ7012697.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(36965..39043)
                     /gene="flhA"
                     /locus_tag="QBH27_000323"
     CDS             complement(36965..39043)
                     /gene="flhA"
                     /locus_tag="QBH27_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDQ7012698.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(39117..40127)
                     /gene="hypE"
                     /locus_tag="QBH27_000324"
     CDS             complement(39117..40127)
                     /gene="hypE"
                     /locus_tag="QBH27_000324"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDQ7012699.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLAPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(40124..41245)
                     /gene="hypD"
                     /locus_tag="QBH27_000325"
     CDS             complement(40124..41245)
                     /gene="hypD"
                     /locus_tag="QBH27_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDQ7012700.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(41245..41517)
                     /gene="hypC"
                     /locus_tag="QBH27_000326"
     CDS             complement(41245..41517)
                     /gene="hypC"
                     /locus_tag="QBH27_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDQ7012701.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(41508..42380)
                     /gene="hypB"
                     /locus_tag="QBH27_000327"
     CDS             complement(41508..42380)
                     /gene="hypB"
                     /locus_tag="QBH27_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDQ7012702.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(42384..42734)
                     /gene="hypA"
                     /locus_tag="QBH27_000328"
     CDS             complement(42384..42734)
                     /gene="hypA"
                     /locus_tag="QBH27_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDQ7012703.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            42946..43407
                     /gene="hycA"
                     /locus_tag="QBH27_000329"
     CDS             42946..43407
                     /gene="hycA"
                     /locus_tag="QBH27_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDQ7012704.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            43532..44143
                     /gene="hycB"
                     /locus_tag="QBH27_000330"
     CDS             43532..44143
                     /gene="hycB"
                     /locus_tag="QBH27_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDQ7012705.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANTNTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            44140..45966
                     /gene="hycC"
                     /locus_tag="QBH27_000331"
     CDS             44140..45966
                     /gene="hycC"
                     /locus_tag="QBH27_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDQ7012706.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCSPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            45969..46892
                     /gene="hycD"
                     /locus_tag="QBH27_000332"
     CDS             45969..46892
                     /gene="hycD"
                     /locus_tag="QBH27_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDQ7012707.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            46910..48619
                     /gene="hycE"
                     /locus_tag="QBH27_000333"
     CDS             46910..48619
                     /gene="hycE"
                     /locus_tag="QBH27_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDQ7012708.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            48629..49171
                     /gene="hycF"
                     /locus_tag="QBH27_000334"
     CDS             48629..49171
                     /gene="hycF"
                     /locus_tag="QBH27_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDQ7012709.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            49171..49938
                     /gene="hycG"
                     /locus_tag="QBH27_000335"
     CDS             49171..49938
                     /gene="hycG"
                     /locus_tag="QBH27_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDQ7012710.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPADILHPDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            49935..50345
                     /gene="hycH"
                     /locus_tag="QBH27_000336"
     CDS             49935..50345
                     /gene="hycH"
                     /locus_tag="QBH27_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDQ7012711.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            50338..50805
                     /gene="hycI"
                     /locus_tag="QBH27_000337"
     CDS             50338..50805
                     /gene="hycI"
                     /locus_tag="QBH27_000337"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDQ7012712.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            50830..51015
                     /locus_tag="QBH27_000338"
     CDS             50830..51015
                     /locus_tag="QBH27_000338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012713.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            51041..51460
                     /locus_tag="QBH27_000339"
     CDS             51041..51460
                     /locus_tag="QBH27_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7012714.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(51552..51989)
                     /locus_tag="QBH27_000340"
     CDS             complement(51552..51989)
                     /locus_tag="QBH27_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012715.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNYDNNVIGLVELIIQKAAGKKITSSKQIDQLDGYQLTSTFSKKL
                     QGRLQTIDDTHEWIMLFEGRAREKAKAYLIELTDKLRVVKTTA"
     gene            52417..52944
                     /gene="hydN"
                     /locus_tag="QBH27_000341"
     CDS             52417..52944
                     /gene="hydN"
                     /locus_tag="QBH27_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDQ7012716.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            53097..55349
                     /gene="hypF"
                     /locus_tag="QBH27_000342"
     CDS             53097..55349
                     /gene="hypF"
                     /locus_tag="QBH27_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDQ7012717.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLTQCLRFVPEWQSYPE
                     TVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LATSCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGGAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(55571..56704)
                     /gene="norW"
                     /locus_tag="QBH27_000343"
     CDS             complement(55571..56704)
                     /gene="norW"
                     /locus_tag="QBH27_000343"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDQ7012718.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGEPQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(56701..58140)
                     /gene="norV"
                     /locus_tag="QBH27_000344"
     CDS             complement(56701..58140)
                     /gene="norV"
                     /locus_tag="QBH27_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDQ7012719.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            58327..59841
                     /gene="norR"
                     /locus_tag="QBH27_000345"
     CDS             58327..59841
                     /gene="norR"
                     /locus_tag="QBH27_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDQ7012720.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            complement(59838..60803)
                     /gene="gutQ"
                     /locus_tag="QBH27_000346"
     CDS             complement(59838..60803)
                     /gene="gutQ"
                     /locus_tag="QBH27_000346"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDQ7012721.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVDGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(60796..61569)
                     /gene="srlR"
                     /locus_tag="QBH27_000347"
                     /gene_synonym="gutR"
     CDS             complement(60796..61569)
                     /gene="srlR"
                     /locus_tag="QBH27_000347"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDQ7012722.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(61636..61995)
                     /gene="gutM"
                     /locus_tag="QBH27_000348"
     CDS             complement(61636..61995)
                     /gene="gutM"
                     /locus_tag="QBH27_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDQ7012723.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(62101..62880)
                     /gene="srlD"
                     /locus_tag="QBH27_000349"
     CDS             complement(62101..62880)
                     /gene="srlD"
                     /locus_tag="QBH27_000349"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDQ7012724.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(62884..63255)
                     /gene="srlB"
                     /locus_tag="QBH27_000350"
                     /gene_synonym="gutB"
     CDS             complement(62884..63255)
                     /gene="srlB"
                     /locus_tag="QBH27_000350"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDQ7012725.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(63266..64225)
                     /gene="srlE"
                     /locus_tag="QBH27_000351"
                     /gene_synonym="gutE"
     CDS             complement(63266..64225)
                     /gene="srlE"
                     /locus_tag="QBH27_000351"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDQ7012726.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(64222..64785)
                     /gene="srlA"
                     /locus_tag="QBH27_000352"
                     /gene_synonym="gutA"
     CDS             complement(64222..64785)
                     /gene="srlA"
                     /locus_tag="QBH27_000352"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDQ7012727.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            65042..66127
                     /gene="mltB"
                     /locus_tag="QBH27_000353"
     CDS             65042..66127
                     /gene="mltB"
                     /locus_tag="QBH27_000353"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDQ7012728.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQSLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            complement(66166..66405)
                     /locus_tag="QBH27_000354"
     CDS             complement(66166..66405)
                     /locus_tag="QBH27_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012729.1"
                     /translation="MAIKKTDNTPQNVLKQTTQMRMWVIIGGISALISVICKVLSSGY
                     LEPFDFGQAALGVVCLGYAISLSKKIKQLRHSNKQ"
     gene            66587..67084
                     /gene="pncC"
                     /locus_tag="QBH27_000355"
     CDS             66587..67084
                     /gene="pncC"
                     /locus_tag="QBH27_000355"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDQ7012730.1"
                     /translation="MTESELMQLSEQVGLALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            67164..68225
                     /gene="recA"
                     /locus_tag="QBH27_000356"
     CDS             67164..68225
                     /gene="recA"
                     /locus_tag="QBH27_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDQ7012731.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            68294..68794
                     /gene="recX"
                     /locus_tag="QBH27_000357"
     CDS             68294..68794
                     /gene="recX"
                     /locus_tag="QBH27_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDQ7012732.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARICQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            68923..71553
                     /gene="alaS"
                     /locus_tag="QBH27_000358"
     CDS             68923..71553
                     /gene="alaS"
                     /locus_tag="QBH27_000358"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDQ7012733.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAVGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            71788..71973
                     /gene="csrA"
                     /locus_tag="QBH27_000359"
     CDS             71788..71973
                     /gene="csrA"
                     /locus_tag="QBH27_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDQ7012734.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            72289..72381
                     /locus_tag="QBH27_000360"
     tRNA            72289..72381
                     /locus_tag="QBH27_000360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72323..72325,aa:Ser,seq:gct)
     gene            72385..72461
                     /locus_tag="QBH27_000361"
     tRNA            72385..72461
                     /locus_tag="QBH27_000361"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72419..72421,aa:Arg,seq:acg)
     gene            72524..72600
                     /locus_tag="QBH27_000362"
     tRNA            72524..72600
                     /locus_tag="QBH27_000362"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72558..72560,aa:Arg,seq:acg)
     gene            72664..72740
                     /locus_tag="QBH27_000363"
     tRNA            72664..72740
                     /locus_tag="QBH27_000363"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72698..72700,aa:Arg,seq:acg)
     gene            73021..73587
                     /gene="yqaB"
                     /locus_tag="QBH27_000364"
     CDS             73021..73587
                     /gene="yqaB"
                     /locus_tag="QBH27_000364"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDQ7012735.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            73584..74012
                     /locus_tag="QBH27_000365"
     CDS             73584..74012
                     /locus_tag="QBH27_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ7012736.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            74085..75641
                     /gene="gshA"
                     /locus_tag="QBH27_000366"
     CDS             74085..75641
                     /gene="gshA"
                     /locus_tag="QBH27_000366"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDQ7012737.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            75791..76306
                     /gene="luxS"
                     /locus_tag="QBH27_000367"
     CDS             75791..76306
                     /gene="luxS"
                     /locus_tag="QBH27_000367"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDQ7012738.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(76357..77469)
                     /locus_tag="QBH27_000368"
     CDS             complement(76357..77469)
                     /locus_tag="QBH27_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ7012739.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWNQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(77466..78173)
                     /locus_tag="QBH27_000369"
     CDS             complement(77466..78173)
                     /locus_tag="QBH27_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HDQ7012740.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGNLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(78431..79969)
                     /gene="emrB"
                     /locus_tag="QBH27_000370"
     CDS             complement(78431..79969)
                     /gene="emrB"
                     /locus_tag="QBH27_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDQ7012741.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(79986..81158)
                     /gene="emrA"
                     /locus_tag="QBH27_000371"
     CDS             complement(79986..81158)
                     /gene="emrA"
                     /locus_tag="QBH27_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDQ7012742.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(81285..81815)
                     /gene="emrR"
                     /locus_tag="QBH27_000372"
     CDS             complement(81285..81815)
                     /gene="emrR"
                     /locus_tag="QBH27_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDQ7012743.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQGGVVLEAMS"
     gene            complement(81906..82241)
                     /gene="ygaH"
                     /locus_tag="QBH27_000373"
     CDS             complement(81906..82241)
                     /gene="ygaH"
                     /locus_tag="QBH27_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDQ7012744.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(82231..>82658)
                     /gene="ygaZ"
                     /locus_tag="QBH27_000374"
     CDS             complement(82231..>82658)
                     /gene="ygaZ"
                     /locus_tag="QBH27_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDQ7012745.1"
                     /translation="LQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSS
                     WSSWVFGTVIGAFSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALV
                     GALAGVTLFSIPVAILAGIVCGCLTALIQAFWQGAPDEL"
BASE COUNT        19563 a        21039 c        22291 g        19765 t
ORIGIN      
        1 ttacggaaat tggagctaac tatgtcaatc gaatctctca atgcgttctc aatggatttt
       61 ttctccctga aaggtaaaac agcaattgtt accggcggga atagcggttt aggccaggca
      121 tttgccatgg cgttggccaa agctggcgca aatgtcttta ttcctagttt cgtcaaagat
      181 aacggcgaaa caaaggaaat gattgaaaaa cagggcgttg aagttgattt tatgcaggtg
      241 gatatcaccg cagaaggcgc gccgcagaag attatcgctg cctgctgtga gcgcttcggt
      301 accgtcgata ttctggtcaa taatgccggt atttgtaagc tgaataaggt gctggacttt
      361 ggtcgtgccg actgggaccc gatgattgac gtgaacctga ccgccgcatt cgagttaagc
      421 tacgaagcgg caaaaattat gatcccgcag aaaagcggta aaatcattaa tatctgttca
      481 ttgttctctt acttaggtgg tcaatggtca cctgcttatt ctgccactaa acatgctctt
      541 gccggattca ccaaagccta ttgcgatgaa ttaggccaat ataatattca ggtgaatggt
      601 attgctcctg gttattatgc aaccgatatt acgctggcga cccgtagtaa tccagaaact
      661 aaccagcgtg ttcttgatca tattccggca aaccgttggg gtgatactca ggatttaatg
      721 ggggcagccg tattcctcgc aagtccggca tcgaattatg tcaacggaca tttattagtg
      781 gttgatggcg gttatttagt gcgctaattc ataacggtgg tttctgtttt ctcttattaa
      841 taactattcc atttttatta tcaggaagga attattatgt ctttatctcg cgtagcgatt
      901 gtcgaccagc taaaggaaat tgttggtgca gatcgcgtta ttaccgatga aacggtatta
      961 aagaaaaaca gtattgaccg ttttcgtaaa tttccggata ttcatggaat ttatactttg
     1021 ccgattccgg cagcggtcgt aaaactcggt tccacagaac aagtatcccg tgtgctgaat
     1081 tttatgaatg cgcacaaaat taacggtgtg ccacgtaccg gtgcttccgc caccgaaggt
     1141 ggactggaaa ccgttgtgga aaactcggtg gtgctcgacg gctccgccat gaatcaaatc
     1201 attaatattg atattgagaa tatgcaggcc acggcgcaat gtggcgttcc gctggaggtg
     1261 ctggaaaacg cattgcgcga aaaaggttac accacggggc attctccgca gtcaaagccg
     1321 ctggcgcaga tgggcggctt ggtggcgacc cgcagtatcg ggcagttctc cacactctac
     1381 ggcgcaatcg aagatatggt cgttggtctg gaagcggtgc tggcagatgg caccgtcaca
     1441 cgcattaaaa aagtgccacg tcgcgcggct ggcccggaca ttcgtcatat catcatcggc
     1501 aacgaaggtg cactgtgcta tatcactgaa gtcacagtga aaatcttcaa atttacgccg
     1561 gaaaacaatc tcttctacgg ctatatcctg gaagatatga aaaccggctt caacatcctg
     1621 cgtgaaatca tggtggaagg gtatcgtccg tcgattgctc gtttgtatga tgctgaagat
     1681 ggcacccaac acttcaccca ttttgccgac ggcaaatgcg tgctgatctt tatggctgaa
     1741 ggtaaccctc gcattgcgaa ggcgacgggt gaagggattg ctgaaatcgt ggcgcgctac
     1801 ccgcagtgcc agcgcgtgga cagcaagctg atcgaaacct ggtttaacaa cctgaactgg
     1861 ggaccggata aagtggctgc cgaacgtgtg cagatcctca aaaccggcaa catgggcttt
     1921 accactgaag tgtccggctg ctggagctgc attcacgaaa tctacgaaag cgttattaac
     1981 cgtattcgta ccgagttccc gcacgccgac gacatcacca tgctgggcgg tcactcctct
     2041 catagctatc agaacggcac caacatgtac ttcgtctacg actacaacgt cgttgactgt
     2101 aagccggaag aggaaatcga caagtaccac aatccgctca acaaaatcat ctgcgaagaa
     2161 accattcgcc tcggcggttc gatggtgcat caccacggta tcggtaaaca tcgcgttcac
     2221 tggagcaaac tggaacacgg cagcgcgtgg gcgttgctgg aagggctgaa aaagcagttc
     2281 gatcctaatg gcattatgaa cacgggtact atctatccga ttgaaaaata atgtgtcagg
     2341 cagcgttccg cgatgacggt gcgttgcctt ttcggcttct caggcgagaa gccgttctta
     2401 ttaccggaca atgaaggggt aaagatgaac acttcaccgg tgcgaatgga tgatttaccg
     2461 cttaaccgtt ttcactgtcg aattgctgcg ctcactttcg gcgcacacct gaccgacggt
     2521 tatgttctcg gcgtcattgg ttatgccatt attcagctta cgcccgccat gcaactgacg
     2581 ccgtttatgg cgggaatgat cggcggctcg gcgttacttg gtttgtttct tggcagcctg
     2641 gttttggggt ggatctccga ccatatcggg cggcaaaaaa tcttcacctt cagttttatg
     2701 ctgattacgc tcgcttcgtt cttgcaattt tttgccacca cgccagagca tcttattgga
     2761 ttacgcattt tgatcggcat tggtctggga ggcgattact cagtcggtca caccttgctg
     2821 gctgaatttt ccccgcgccg ccatcgcggt attttgctgg gcgcattcag tgtggtgtgg
     2881 accgtaggct atgtgctggc aagtattgcc ggacatcact ttatttccga aagcccggag
     2941 gcctggcgct ggctgctggc atcagcggcg ttaccagcct tgctgattac gctgctacgt
     3001 tggggcacgc cggaatcgcc acgctggcta ctgcgccagg ggcgttttgc agaagctcac
     3061 gctatcgttc atcgctattt tggcccgcat gttttactgg gcgatgaagt ggcaacggcg
     3121 acccataaac acatcaaaac cttgttctct tcgcgctact ggcggcgcac agcgtttaac
     3181 agcgtcttct ttgtctgcct cgtaatccca tggtttgtga tttatacctg gctgccaact
     3241 atcgcccaga ctattggact ggaagatgcg ctgactgcca gtctgatgct taatgcgttg
     3301 ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc tgctggcaca tcgcaagttt
     3361 ttgctgggaa gttttttgct gctggcggca acgctggtag tgatggcctg tttgccttcc
     3421 ggcagttcat taacgctgct gctttttgtt ctcttcagca ccaccatttc ggcagtcagt
     3481 aatctggtgg gcattttgcc tgcggaaagt tttcctactg acattcgctc gctgggcgtc
     3541 ggttttgcca ccgccatgag tcggttgggg gcggcggtaa gtactggcct gctgccgtgg
     3601 gtgctggcgc agtggggaat gcaagccacc ttattactcc tggcggcagt gttgttggtt
     3661 ggttttgttg tgacctggct atgggcacca gaaaccaaag cactcccgct ggtggcggcg
     3721 ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt aaagccgaac cggatgccgg
     3781 aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt aatagcggac cggatgtttc
     3841 gcttctgcga agtttactcg gtgctgatga acaggccgca gctgcgctgc tgttggcgca
     3901 gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc atgggggatg aacgggcgtt
     3961 gcactggctg cgctatctca tggctcttgg gtttgaggaa gctgtattgc tggagacggc
     4021 ggcagatctg cgctttgcgc cggaatttgt cgctcgccat attgccgaat ggcagcgtca
     4081 gaatccgctg gatctgataa ttaccggctg ccaaagcagc gaagggcaga atgggcaaac
     4141 gccatttttg ctggcggaaa tgctggtctg gccctgcttc acccaggtgg agcgctttac
     4201 tctcgacgca ccgtttatca ccctcgaaca gcgtactgaa catggggtgc gctgttgccg
     4261 ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt gaagtggcct tgccagttcc
     4321 gggaatgcgc cagcgaatgg ctgcggggaa agcagcaatt atccgtaaaa ctgttgctgt
     4381 cgaattgcca gcgatgcagt gtctgcaact ggcaagagca gagcaacgac gcggagcgac
     4441 gctgattggt gggcaaacgg tagcagaaaa agcgcaaaag ctatggcagg actatttacg
     4501 ccaaaggatg cagccatgaa tatcgtaatt gtgacaataa atcaggacca tgccgcaata
     4561 gcgagctggc tggcggcgca ggatttcagc ggttgtaccc tggcacattg gcaaatagaa
     4621 ccacagccga tggttgcgga acaggtgctg gatgcactgg tggaacagtg gcaacgaacg
     4681 cctgcggacg ttgtcctttt tcctccgggc gcgtttggcg atgaactgtc gacccggctg
     4741 gcctggcgtt tacatggtgc aagcatctgc caggtgactt cgttagatat tccaaccgta
     4801 agcgtgcgca aatcccactg gggaaatgcg ttgacggcga cgctgcaaac agacaaacgc
     4861 ccgctatgcc tttcgctggc acgtcaggcg ggagcagata aaaacgccac gttgccctct
     4921 gggatgcagc aactgaacat tgtcccaggt gtgcttcccg actggctggt tagcatagaa
     4981 aatctgaaaa atgttatccg cgacccatta gctgaagcca gacgggtgct ggtggtgggg
     5041 cagggcggag aggcagataa ccaggaaatc gctatgctgg cagaaaagat gggagccgaa
     5101 gtgggctaca gtcgggcacg agtgatgaac ggtggcgttg atgccgaaaa agtgattggt
     5161 atttccggac atctgctggc acctgaggtg tgtattgttg ttggcgcttc cggcgcagca
     5221 gcgttaatgg cgggcgtacg caatagcaag tttgtggtgg cgattaatca tgacgccagt
     5281 gcagcggtat tttcacaggc agatgttggc gtagttgatg actggaaagt ggtgctggag
     5341 gcactggtga caaacattca cgcagattgc caataacgtg ctgccggatg atcctgccga
     5401 tggttctggg ctggggactt gtaggcctga taagacgcgt caagcgtcgc atcaggcgct
     5461 gaatgtcgga tgtggcgtgc agcctacaaa tcacaccaaa ttcaacttgt tacgattatc
     5521 ccgtcaaagc aattttttcg ctaacgtcca gaccccagta ttcgtggtgg aaagcgccac
     5581 aacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg cacaccagcc cgccagcaat
     5641 cagcggttgg cggattttct ccgtcaccca gcccagcact ttaggcatgc agcctggcag
     5701 gatctcaata caatccggat tcgattgcgc aacttgctta tcaatgttgt gaaacgaaat
     5761 cgaatcaaca ataaacagtc gatgaataca gaaaaaacct tccgctcttg ctgctttcag
     5821 cattgaggct ttggtactga taattccgtc cgcctcggtt accagtttca aaaactgaat
     5881 caccacttct ttatttgacg cgccttccag caaatcaaca tgaataaaag catatttccc
     5941 ggcgttctta atctttttaa cgatattact aatggtgcag atattgccgt acaacacgga
     6001 aataaactgg cattcagagt cgattgccag ttgcaggctg gcattatctt taacggcagc
     6061 aatcaccgga ttctggcgga gcaggtgtaa gaggggcata aattatcctt attatttatc
     6121 caaagcggta ggtgatgccg aatccggaag gcggatagcg ccactgttgt agtgttgatt
     6181 cgtcgcacag caaacggcag gtgccacact ccaggcagcc gtgataatca acacgtaagc
     6241 caccttcagg tgtgagcgaa aaaagacctg ctggacaggc gttaatcaac cgttgtgccg
     6301 tctggcgctc aacggagtca gtcggaacaa tgtgcggcgc atcagcagcg cgccagagat
     6361 tacgggctac agacatcgca gactcctgat aacatcgccc gtcagatgcc acaggccgtg
     6421 acgacgtaaa tgacgccaga gcaactggcg cagcggtgga acaggtgtat tcccctgatc
     6481 ccataaatca cgggaaatat cctgcattaa cccaggccac acccgatacc agccaggacg
     6541 ttgcaaaagc gccggaacat gctgataacg ctgtagaaca tcccacagca ggctgcgctc
     6601 tacgttgtga tgataaagcg gaaacagatt ttgcggctcg cggtgttggc aggcgcttat
     6661 cagcgtttgt gccgccgcct gcgcgccagt tagcgccata tccatgccgc gcacggaaat
     6721 tccggtattg acgcaactgc gcaacgcatc gcccaccagc agccagccgt taccggcgta
     6781 ttgcaccggc atactgcgca ggccaccttc tggcaccaga tgcgcaccat actccagtaa
     6841 ttccgtgttt ttgataagcg ggcgcactgc cggatgcgtt ttaaagcgag tcagcagctc
     6901 gcttgccgga acactacttt gcgtaaggga ggagagcggg caaacaatcc ctaacgagag
     6961 cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag tcatcacaga tcccgccgct
     7021 gaacaacaac gctgcgcctt cgttattctc cagatgaaaa cgttcttcaa tagcggatgg
     7081 ttccagcgtc agcacttctt tgattcccaa tgccatcgct tcgccagcag gacgagtcac
     7141 taacccgtgg cgttccgcca ggacgctgtt ggcaccttct gccagcacca cataacgggc
     7201 gtgaagaata tcgtcaccac aaatgacacc acacaccctg ccgttttctt catacagtgc
     7261 atctaccgtc gcaccgggga tgcattcgac accttctttt tcggcttcgg caaccagcca
     7321 cggatcgaat cgtgcacgta atacactcca ggattcaccg ccgggctgta agctggaaaa
     7381 cgtcgttgcg ccatctggcg ttaacagaga aagactttcg tgagtgatgc gtcgttcaag
     7441 gggcgcggtc aggtgaaatt gcgggaggag ttccgcgagc gcatgggtat ataaacgccc
     7501 gccggaaaga tttttgctgc cgggaatctc cgctcgttcc agtaacaaaa cggataaacc
     7561 cgctcgcgcg cagcgtaacg cgcaagcggt gccagcaatt ccggcaccga taataataat
     7621 gtcgcagtcg tcttccatgt catgccccag atatccagaa cacaacaccc taacatagcg
     7681 ttacttaagg gaaattgacc gccgacacac tcttatcatt cgccgcgata gatacaacct
     7741 gcggtgcagg tttcttttac catcaccgca cttaacagcg gcacaacggg tttaacctga
     7801 tcccaaatcc attttgctaa aacttcgctg gttgggtttt ccagacctgg aatatcattg
     7861 agataatggt gatcgaggcg ctcataagtt ggtttaaacg ccgctttcag ttcagcgaaa
     7921 tcgataatcc agcccgtatg cggatcgact tccccggtaa tttccagtcg caccataaag
     7981 gaatgcccgt gcaggcgacc acatttatgc ccttgtggga cgtgcggcaa gcggtgagcg
     8041 gcttcgaagg tgaaatcttt aaataacgtg gtggacatca taatttctct acatgacgat
     8101 aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat tatctaaaac gactttgttg
     8161 cgcaaaatcg ctgatttatc ttattgatgg gcaatattca tttgtttttc attgggttgg
     8221 ttaatctatt ttgttgttaa agactattgc taaaacaggt tagtcgattt ggttattagt
     8281 tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt aaccttacct tctcttctgt
     8341 tttatgggcg ctgacagggc gcagaaacag ctttgcttac tggaacataa cgacgcatga
     8401 cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc ggagcaactg gcacgccttc
     8461 aggcggccac gaccgattta actcctaccc agcttgcctg ggtttctggc tatttctggg
     8521 gcgtgctcaa ccagcagcct gctgcgcttg cagcgacgcc agcgcccgcc gcagaaatgc
     8581 cgggtataac tattatctcc gcctcgcaaa ctggcaatgc gcgtcgggtt gctgaagcat
     8641 tacgcgatga tttattagcg gcaaaactga acgttaagct ggtgaacgcg ggcgactata
     8701 aattcaaaca aatcgccagc gaaaaactgc ttatcgtggt gacgtcaacg caaggggaag
     8761 gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct gttctccaaa aaagcgccaa
     8821 agctggaaaa caccgcgttt gccgtgttta gcctcggcga tagctcttat gaatttttct
     8881 gccagtccgg gaaagatttt gacagtaagc tggcggaact gggcggtgaa cgcctgctcg
     8941 accgtgtcga tgccgacgtt gaataccagg ctgctgccag cgagtggcgc gcccgcgtgg
     9001 ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc gcaatccgtc gctactggca
     9061 cggtaaatga aatccacacc agcccgtata gcaaagacgc gccgctggtg gcgagcctct
     9121 ctgttaacca gaaaattacc gggcgtaact ctgaaaaaga cgttcgtcac attgaaattg
     9181 acttaggtga ctctgacctg cgttaccagc cgggtgacgc gctgggcgtc tggtatcaaa
     9241 acgatccggc actggtgaaa gaacttgtcg aactgctgtg gctgaaaggc gatgaacctg
     9301 tcaccgtcga gggcaaaacg ttgcctctga acgaagcgct acagtggcac ttcgaactga
     9361 ccgtcaacac cgccaacatt gttgagaact atgccacgct tacccgcagc gaaacgctgc
     9421 tgccgctggt gggcgataaa gcgaagttac agcattacgc cgcgacgacg ccgattgtcg
     9481 acatggtgcg tttctctccg gcgcaactgg atgccgaagc gctgattaat ctgctgcgcc
     9541 cactgacccc gcgcctgtat tccatcgcct cctcgcaggc ggaagtcgag aacgaagtgc
     9601 acgttaccgt tggtgtggtg cgttacgacg tggaaggccg cgcccgtgcc ggtggtgcct
     9661 ccagcttcct cgcggatcgt gtggaagaag agggtgaagt ccgcgtattt atcgaacata
     9721 acgacaactt ccgcctgcca accaatccgg aaaccccggt gatcatgatt ggccctggca
     9781 ccggcatcgc gccgttccgt gcctttatgc agcagcgtgc cgccgacgaa gcgccgggta
     9841 aaaactggct attctttggc aacccacact ttacggaaga tttcctctac caggtggaat
     9901 ggcagcgtta cgtcaaagaa ggcgtgctga cgcgcatcga tctcgcctgg tcgcgcgatc
     9961 aaaaagaaaa aatttacgta caagacaaac tgcgcgaaca gggcgcagag ctgtggcgct
    10021 ggatcaatga cggtgcccac atttatgtct gcggcgacgc taatcgcatg gcgaaagacg
    10081 ttgagcaggc acttctggaa gtgattgccg aatttggtgg catggacacc gaagcggcgg
    10141 atgaattttt aagtgagctg cgcgtagagc gccgttatca gcgagatgtc tactaatgag
    10201 cgaaaaacat ccagggcctt tagtggtcga aggaaaactg acagacgccg agcgcatgaa
    10261 gcttgaaagc aactacctgc gcggcaccat tgcggaagat ttaaacgacg gtctgaccgg
    10321 cggctttaag ggcgataact tcctgctgat ccgcttccac ggcatgtatc agcaggatga
    10381 ccgcgacatt cgcgccgaac gtgctgaaca gaagctggag ccgcgccacg cgatgctgct
    10441 tcgctgtcgt ctgccgggtg gggtgattac cactaaacag tggcaggcga tcgacaaatt
    10501 tgccggtgaa aacaccatct atggcagcat tcgcctgacc aaccgccaga cgtttcagtt
    10561 ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag atgctgcact cggtcggtct
    10621 tgatgcgctg gcgaccgcca acgacatgaa ccgtaacgta ctctgcacct cgaaccctta
    10681 cgagtcgcag ctgcatgcgg aagcatatga atgggcgaag aaaatttctg aacatctgct
    10741 gcctcgtacc cgcgcgtatg cggagatctg gctcgatcag gaaaaagtcg ccactactga
    10801 tgaagagccg atccttggtc agacctacct gccgcgtaag ttcaaaacca cggtagtgat
    10861 cccgccgcag aacgatatcg atctgcacgc caacgacatg aacttcgtgg cgatcgccga
    10921 aaacggcaag ctggtgggct ttaacctgct ggtgggcggt gggctttcca tcgaacacgg
    10981 caacaagaaa acctacgccc gcacggcgag tgagtttggc tatctgccgc tggagcatac
    11041 gctggcggtg gcggaagccg tcgtgacaac tcagcgtgac tggggtaacc gaaccgatcg
    11101 taaaaatgcc aaaaccaaat acacgctgga gcgcgtaggg gttgagacgt ttaaagcgga
    11161 agttgaacgt cgcgcgggga tcaaattcga gccgattcgc ccgtatgagt ttaccggacg
    11221 cggcgatcgt attggctggg ttaagggcat tgatgataac tggcacctga cgctgtttat
    11281 cgaaaatggt cgaattcttg attatccggg gcgcccgctg aaaaccggct tgctggagat
    11341 cgcgaagatc cacaaaggtg atttccgcat tacggcgaac cagaatctga tcatcgccgg
    11401 cgtgccggaa agcgagaaag cgaagatcga gaagatcgcc aaagaaagcg ggttaatgaa
    11461 tgccgtcacg ccgcagcgtg aaaactcaat ggcctgcgtg tcattcccga cttgcccgct
    11521 ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc gacaacatcg ataatttaat
    11581 ggcgaaacat ggtgtcagcg atgagcatat cgtgatgcgt gtaacaggct gcccgaacgg
    11641 ttgtggtcgc gcgatgctgg cggaagtggg tctggtgggg aaagcgccgg gtcgctacaa
    11701 cctgcatctt ggcggcaacc gcattgggac acgtatccca cggatgtata aagaaaacat
    11761 caccgagccg gaaatcctgg cgtcgcttga tgaactgata gggcgctggg cgaaagagcg
    11821 cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg ggcatcattc gcccggtgct
    11881 cgatccggcg cgcgatttgt gggattaacc atcagcccgg tcttgtaggc ctgataagac
    11941 gtgtgagcgt tgcatcaggc aaggcaaaca gtgaggaact tatgtccaaa ctcgatctaa
    12001 acgccctgaa cgaactgcca aaggtagacc ggattctggc gctggcggaa actaacgccc
    12061 aactggaaaa actggacgct gaaggccgcg tagcctgggc gctggataat ctgcccggtg
    12121 aatatgtact ttcttccagc tttggcattc aggcggcggt gagcctgcat ctggtgaatc
    12181 agattcgccc ggatattcca gtgatcctca ccgataccgg ctatctgttc ccggaaacct
    12241 accgctttat tgacgagtta acggacaaac tcaagctcaa cctgaaagtg tatcgtgcta
    12301 ccgaaagcgc cgcctggcag gaagcacgct acggcaaact gtgggagcag ggcgttgaag
    12361 gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat gaaccgggcg ctgaaagaac
    12421 tgaatgcgca aacctggttt gctggcctgc gccgtgaaca atccggcagc cgcgccaatt
    12481 taccggtgct ggcaattcag cgtggcgtat ttaaagtgct gccgattatc gactgggata
    12541 accgaactat ttatcagtac ctgcaaaaac atggcctgaa atatcaccca ttatgggatg
    12601 aaggatattt atcggtgggc gatacccata caacccgtaa atgggaaccc ggcatggcgg
    12661 aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg attacacgaa gggtaaatat
    12721 tgccggataa attaaacgcc tctgtcagaa atgatggagg cgtttttatt ttttgcgagc
    12781 agcattccgg catttaaagg tgaacatcgc agggaaaatt aacaggtata gaatggcatt
    12841 gggtgcttga ggctgtctgg cgttaagcat tcgcaaggtt ccagatggac aaaagcccca
    12901 ggcgatattt ctatcaacct gaggctcgcg ttcgaacgta gcaattcgga tgttagtctc
    12961 ttctttgcag ggaggcaaga catgctgaca aaatatgccc ttgtggcaat catcgtactg
    13021 tgttgtacag tactgggatt cacgttgatg gtaggcgact cgttgtgtga gttgagtatc
    13081 agagaacgtg gtatggagtt taaggcagtt ctcgcttacg aatcgaagaa gtagccacca
    13141 cgcggggtaa aaccccgcgt actgaattgt gatggtttgg cctcctgcac ccctttttta
    13201 tactctgttt ctggtggcat tgatttcctg caggcaaggg agtaatcatt tcaaacccat
    13261 taagatgcct tcgccagctc cttcaccaac ggtagcatta tccgcataac atcacggcag
    13321 cgacgttcta ttcttccagg aagtgcctta tcaatatgct gttgattatc cagtcttacg
    13381 tcatgccagc tatttcccgc agggaatgcg gctgtttttg ctcgttgctg ataaccatcc
    13441 ttattgccaa gattccagtt agtcgcttcc accgaaagta cagcaatacc ggctttgtcg
    13501 aatatttctg cgtcattaca acacccggtg cctttcggat agtttttatt caaacctgga
    13561 tttgtggtgg cggcaattcc atggctgcgg gcaattgcca gcgcccggtc gcgcgttaat
    13621 ttccttactg cttcaggggt ttttacaccg ctgttgaaat acaatttatc gccaacaatt
    13681 aagttatcga gattaatcac cagcagcgta tttttctttt cggtgtcact catccgcttg
    13741 agtaaattct cagcgcctaa ttttccttcc tcttcgccgc tggtcgccac aaatcgaata
    13801 ccatactcgg taggcgtatt tttcaggcgc tcggccagtt ccagcatgac acctaaacct
    13861 gcggcgttat catccattcc ttgtaacgtc agcccgccga gattggcatc ggcgtcagca
    13921 tcgctcagcg gggcgtaagt atccagatgc gccataatga tgatctgctg cggcgttttg
    13981 ccttcatgag cggcaatcac cgtacttccc gtcacgttat gccagctctt gcgattatcg
    14041 cgggcggtat aaatataccg actattaaat gtccgaatat cactgcgata acccatttgc
    14101 tgaaactgtt ggcgaatata atcggcagat aacatttctg caggagttcc ggtcatgcgt
    14161 cccggaaaga aagtagcaat atgtcgtgcc tgggtattag caaaatcgcc aggtttaggt
    14221 gacgaggcgt gtacggggag aataaagcat acgccgagcg ccagggcagc ggtacggtgg
    14281 cgcaatgcgg aaaacatagt gagtccttaa ataccatgca aattttttta ccgccatagt
    14341 atgaaactgc cgctgcgcta aaacaatttc aaatcttcct aaacgcccga aatccggtgc
    14401 cttaagcact ttttgatatt agctttgcca aatcgttatt ccgttaagga actactcatt
    14461 ctaattggta atttcattcg ttctcttacg ctccctatag tcgaaacatc tgatggcaag
    14521 aaaatagcgg tattgcaaag gaacggttat ggatcaaaaa cgacttaccc acctgcggca
    14581 actggaggcg gaaagcatcc acattattcg cgaggtggcg gcagaattct caaatccggt
    14641 gatgctctac tctatcggta aagattccag cgtcatgctg catctggcgc gcaaggcgtt
    14701 ttatccaggt acgctgcctt tcccgttgct gcatgtcgat accggctgga aattccgcga
    14761 gatgtatgag ttccgcgatc gtacggctaa agcctacggc tgcgagctgc tggtgcataa
    14821 aaacccggaa ggcgtagcga tggggattaa tccattcgtt catggcagcg cgaaacatac
    14881 cgatattatg aaaactgaag gcctgaaaca ggcgctgaac aaatacggtt ttgatgccgc
    14941 cttcggtggt gcgcgccgcg acgaagagaa atcccgcgct aaagagcgaa tctactcttt
    15001 ccgtgaccgc ttccatcgct gggatccgaa aaatcagcgc ccggagctgt ggcacaacta
    15061 caacgggcaa attaacaaag gcgaaagcat ccgcgtcttc ccgctctcta actggaccga
    15121 gcaggatatc tggcaataca tctggctgga aaatattgac attgttccac tgtatctcgc
    15181 tgcggaacgt ccggttctgg aacgcgacgg tatgttgatg atgattgatg acaaccgtat
    15241 cgacctgcaa ccgggcgaag tgattaaaaa acggatggtg cgtttccgta cgctgggctg
    15301 ctggccgctg accggcgcgg tggagtcaaa cgcacaaaca ctgccggaga tcatcgaaga
    15361 gatgctggtt tccaccacca gtgaacgtca gggccgcgta attgaccgcg accaggcggg
    15421 gtctatggag ctgaaaaaac gtcaggggta tttttaagat gaacaccgca cttgcacaac
    15481 aaatcgccaa tgaaggcggc gtcgaagcct ggatgattgc gcaacaacat aaaagcctgc
    15541 tgcgttttct gacctgtggt agcgtcgatg acggcaaaag taccctgatt ggtcgcctgc
    15601 tacacgatac ccgccaaatc tacgaagatc agctctcatc gctgcataac gacagtaagc
    15661 gtcacggcac ccagggcgaa aagctggatc tggcactgct ggtggacggc ctgcaagctg
    15721 agcgcgaaca gggcatcacc attgacgtgg cttaccgcta tttctctacc gagaagcgta
    15781 aatttattat cgccgacacc ccagggcacg agcagtacac ccgcaatatg gcgactggcg
    15841 catcgacatg tgaactggcg atcttactga tcgatgcccg taaaggcgtg ctcgatcaaa
    15901 cccgtcgtca cagttttatc tccacactgt tggggatcaa acatctggtc gtggcgatca
    15961 acaaaatgga tctggtggat tacagtgaag agacgttcac ccgtattcgt gaagattatc
    16021 tgacctttgc cgggcagctg ccgggtaatc tggatatccg ctttgtgccg ctctccgcac
    16081 tggaaggcga caacgtggct tcgcaaagtg aaagtatgcc gtggtacagc ggtccgacac
    16141 tgctcgaagt gctggaaacc gtggagatcc agcgagtggt ggatgctcag ccaatgcgct
    16201 tcccggtgca gtacgttaac cgtccgaatc tcgattttcg tggctacgcc ggaacgctgg
    16261 catccggtcg tgtggaagtc gggcaacgtg tcaaagtgct gccctctggt gtggaatcaa
    16321 acgtcgcgcg gatcgtgacc tttgatggcg atcgcgaaga agcctttgcc ggagaagcga
    16381 tcaccctggt gctgacggat gagatcgaca tcagccgtgg cgatctgctg ctggcggcag
    16441 acgaagtgtt accggctgtg cagagcgcgt cggtggatgt ggtatggatg gcggaacagc
    16501 cgctttcccc gggccagagt tacgacatca aaattgccgg taagaagacg cgtgctcgtg
    16561 ttgatggcat tcgttatcag gttgatatca ataaccttac ccaacgcgaa gttgaaaacc
    16621 tgccgctgaa cggcatcggc ctggtagatc tcacctttga cgagccactg gtgttagatc
    16681 gttatcagca aaacccggtt accggtgggc tgatttttat cgatcgcctg agtaatgtga
    16741 ccgtaggtgc cggtatggtg cacgaaccag ttagccaggc aactgctgcg ccatctgaat
    16801 tcagtgcatt cgaactggaa ttgaatgccc tggttcgccg ccatttcccg cattggggtg
    16861 cgcgcgattt actgggagat aaataatggc tctgcatgac gaaaacgtcg tctggcatag
    16921 ccacccagta actctgcaac aacgcgaaca gcaccacggt catcgtggtg ttgtgctgtg
    16981 gtttaccggc ctctctggtt ccggtaaatc aaccgtcgcg ggggcgctgg aggaggcgtt
    17041 acataaactt ggcgtcagta cgtatctgct ggatggcgac aacgttcgcc acggattatg
    17101 cagcgatctc ggttttagcg atgccgaccg taaagagaat atccgtcgcg tcggtgaagt
    17161 ggcgaatttg atggttgaag ccggactggt ggtgctgacc gcatttatct cgccacaccg
    17221 cgccgaacgc cagatggttc gcgaacgcgt aggagaaggg cgctttatcg aagtgtttgt
    17281 cgatacgccg ctggcgattt gcgaagcccg cgatccaaaa ggtttatata agaaagcgcg
    17341 tgccggtgaa ctgcgcaact ttacgggaat agattccgtt tacgaagcgc ctgaatcggc
    17401 agaaattcat ctcaatggtg aacaattagt aacaaatttg gtacagcaat tattagatct
    17461 gttgagacag aacgatatta tcagatcctg agacaccacc gggtttcatg cccggttcgt
    17521 caaagtaaca ggatcagata tgcgtaacag ccacaacatt acattaacaa ataacgacag
    17581 ccttactgag gttgaagaca ccacatggtc gctgccaggc gctgtggttg gttttttatc
    17641 gtggctgtgc gcgttggcaa ttccgatgtt gatttatggc tctaacacgc tgctcttttt
    17701 tatctacacc tggcctttct ttctggcgct gatgcccgtc gctgtagtgg tggggattgc
    17761 actgcattca ttgatggacg gaaagctacg ctacagtatt gttttcactc tggcgacggt
    17821 tggcattatg tttggtgcac tgtttatgtg gctattgggt taattgttct ttcccgtcta
    17881 ttctgttcat aattcaatcc gtaactaata atgagattat tttctgcacg ccctgggtat
    17941 acgtaacaat ggacaaatgt ggtacatttg cccgcgttgt cgcggtatcc ccaacagagg
    18001 atgtagagtc gtcttcggat gcatgggatg aagatgccgt ttttcagggg gcaggatggg
    18061 taaactaacg ctgctgttgc tggctattct ggtctggcta cagtattcgc tgtggttcgg
    18121 taagaacggt atacatgact atactcgcgt caataatgat gtggcggcac agcaagctac
    18181 aaacgcgaaa cttaaagcgc gaaacgatca gctttttgcc gaaattgacg atctcaatgg
    18241 cggccaggag gcgctcgaag agcgtgcgcg taatgaactc agcatgacca ggccgggcga
    18301 aacgttttat cgtctggtgc ctgacgcgtc aaagcgcgca cagtctgcgg ggcaaaacaa
    18361 tcgataaatc agcccgggaa ttaacatggc aaccactcat ttggatgttt gcgccgtggt
    18421 tccggcggcc ggatttggcc gccgaatgca aacggaatgt cctaagcaat atctctcaat
    18481 cggtaatcaa accattcttg aacactcggt gcatgcgctg ctggcgcatc cccgggtgaa
    18541 acgtgtcgtc attgccataa gtcctggcga tagccgcttt gcacaacttc ctctggcgaa
    18601 tcatccacga atcacggttg ttgatggtgg tgaagagcgt gccgattccg tgctggcagg
    18661 tctgaaagcc gctggcgacg cgcagtgggt attggtgcat gacgccgctc gtccttgtct
    18721 gcatcaggat gacctcgcgc gattgttggc gttgagcgaa accagccgca cgggagggat
    18781 cctcgctgcg ccggtgcgcg atacgatgaa acgtgccgaa ccgggcaaaa atgccattgc
    18841 tcataccgtt gatcgcaacg gcttatggca cgcgctgacg ccgcaatttt tccctcgtga
    18901 gctgttacat gactgcctga cgcgcgctct aaatgaaggc gctaccatta ccgatgaggc
    18961 ctcggcgctg gaatattgcg gttttcatcc gcaacttgtt gaaggccgcg cggataacat
    19021 caaggtaacg cgcccggagg atttggcgct ggccgaattt tacctcaccc gaaccatcca
    19081 tcaggagaat acataatgcg aattggacac ggttttgacg tacacgcctt tggcggtgaa
    19141 ggcccaatta tcattggtgg cgtacgcatt ccttacgaaa aagggctgct ggcgcattct
    19201 gatggcgacg tggcgctgca tgcgttgacc gatgcattgc ttggcgcggc ggcgctggga
    19261 gatataggca agctgttccc ggataccgat ccggcattta aaggtgccga cagccgcgag
    19321 ctgttgcgcg aagcctggcg tcgtattcag gcgaaaggtt atgccctcgg caacgtcgat
    19381 gtcactatca tcgctcaggc accgaagatg ttgccgcaca ttccacaaat gcgcgtgttt
    19441 attgccgaag atctcggctg ccatatggat gatgttaacg tgaaagccac tactacggaa
    19501 aaactgggat ttaccggacg tggggaaggg attgcctgtg aagcggtggc gctactcatt
    19561 aaggcaacaa aatgattgag tttgataatc tcacttacct ccacggtaaa ccgcaaggca
    19621 ccgggctgct gaaagccaat ccggaagact ttgtggtggt ggaagatttg ggctttgagc
    19681 ctgatggtga aggtgagcat attctggtta gaatcctcaa aaatggctgc aatacccgtt
    19741 ttgtggcgga tgcactggcg aaattcctga aaattcatgc ccgtgaagtc agcttcgctg
    19801 ggcaaaaaga taaacatgct gttacggaac agtggttatg cgctcgcgtg ccgggcaaag
    19861 agatgcccga tctgagcgcc tttcaactgg aaggctgcca ggtgctggag tatgcgcggc
    19921 acaagcgcaa gctgcgttta ggcgcgctga aaggtaacgc ctttaccctg gttctgcgcg
    19981 aagtgagcaa tcgcgatgac gttgaacaac gtctgatcga tatttgcgta aaaggtgtac
    20041 cgaactactt cggtgcccaa cgttttggga ttggcggtag caacttgcag ggcgcgctgc
    20101 gctgggcgca aaccaatact ccggtgcgcg atcgcaataa acggagtttt tggttgtcgg
    20161 cagcccgcag tgcgttgttt aatcagattg ttgctgagcg cctcaaaaaa gcagacgtta
    20221 atcaagttgt tgacggcgat gcgctacaat tagccggacg tggtagctgg tttgttgcaa
    20281 ccaccgaaga actggcggaa ttacagcgtc gcgtcaacga taaagagcta atgataaccg
    20341 ccgcattgct gggcagtggc gaatggggaa ctcagcgtga agcgctggca ttcgaacaag
    20401 cagctgtcgc cgaagaaact gaattacaaa ctttactggt gcgtgaaaaa gttgaagccg
    20461 cgcgcagagc gatgctgctg tatccgcaac aattaagctg gaactggtgg gatgacgtca
    20521 ccgtagaaat ccgtttctgg cttccggcgg gaagttttgc aaccagcgtt gtcagggaac
    20581 ttatcaacac aacaggtgat tatgcgcata ttgctgagta atgatgacgg ggtacatgca
    20641 cccggtatac aaacgctggc gaaagccttg cgtgagtttg ctgacgttca ggtggtcgcc
    20701 cccgatcgta accgcagcgg cgcttctaat tctctgacac tggaatcttc cctgcgcacg
    20761 tttacctttg aaaatggtga tattgctgtg caaatgggaa ccccgaccga ttgcgtctat
    20821 cttggcgtaa atgctctgat gcgcccgcgc ccggacattg ttgtgtccgg aattaacgcc
    20881 gggccgaatc tgggggatga tgttatttat tccggtacgg tagcggccgc gatggaaggc
    20941 cgtcatttag gttttccggc gcttgccgtc tcgcttgacg ggcataaaca ttacgacact
    21001 gccgcggcgg taacctgctc aattttgcgc gcattgtgta aagagccgct gcgcaccggg
    21061 cgtattctta atattaacgt tccggattta ccattggatc aaatcaaagg tattcgcgtg
    21121 acgcgctgcg gtacacgaca tccggcagat caggtgatcc cgcagcaaga tccgcgcggc
    21181 aatacgctgt actggattgg cccgccgggc ggtaaatgtg atgccggtcc ggggaccgat
    21241 tttgctgcgg tagatgaggg ctatgtttcc atcacgccgc tgcatgtgga tttaactgcg
    21301 catagcgcgc aagatgtggt ttcagactgg ttaaacagcg tgggagttgg cacgcaatgg
    21361 taagcagacg cgtacaagca cttctggatc aattacgtgc gcaaggtatt caggatgagc
    21421 aggtgctgaa tgcacttgcc gccgtgccgc gtgaaaaatt cgttgatgaa gcgtttgaac
    21481 aaaaagcctg ggacaatatc gccttgccga taggtcaggg gcagacaatt tcgcagccgt
    21541 atatggtggc gcgaatgacc gaattactcg agctaacgcc gcagtcgcgg gtgctggaaa
    21601 ttggcactgg ttcgggatat caaactgcaa tcctggcgca tcttgtccag catgtttgct
    21661 cggttgaacg gattaaaggc ttgcagtggc aggcgcgtcg ccgcctgaaa aatcttgatt
    21721 tacataatgt ttcaacccgt catggcgatg gatggcaagg ttggcaggca cgcgcgccgt
    21781 ttgacgctat cattgttacg gcggcaccgc ctgaaattcc aactgcgcta atgacgcagc
    21841 tggacgaagg cgggattctc gtcttacccg taggggagga gcaccagtat ttgaaacggg
    21901 tgcgtcgtcg gggaggcgaa tttattatcg ataccgtgga ggccgtgcgc tttgtccctt
    21961 tagtgaaggg tgagctggct taaaacgtga ggaaatacct ggatttttcc tggttatttt
    22021 gccgcaagtc agcgtatcgt gaacatcttt tccagtgttc agtatggtgc cttgcacggt
    22081 aattatgtca ctggttatta accaattttt cctgggggat aaatgagcgc gggaagccca
    22141 aaattcaccg ttcgccgcat tgcggcattg tcactggttt cgctatggct ggcaggctgt
    22201 tctgacactt caaatccacc ggcaccggtc agctccgtta atggcaatgc gcccgcaaat
    22261 accaattctg gtatgttgat tacgccgccg ccgaaaatgg ggacgacgtc tacagcgcag
    22321 caaccgcaaa ttcagccggt acagcagcca caaattcagg ctactcaaca accgcaaatc
    22381 cagcctgtgc agccagtagc tcagcagccg gtacagatgg aaaacggacg aatcgtctac
    22441 aaccgtcagt atgggaacat tccgaaaggc agttatagcg gcagtacata caccgtgaaa
    22501 aaaggcgaca cgcttttcta tatcgcctgg attactggca acgatttccg tgaccttgct
    22561 cagcgcaaca atattcaggc accatacgcg ctgaacgtcg gtcagacctt acaggtgggt
    22621 aatgcttccg gtacgccaat cacaggcgga aatgccatta cccaggccga cgcagcagag
    22681 caaggagttg tgatcaagcc tgcacaaaat tccaccgttg ctgttgcgtc gcaaccgaca
    22741 attacgtatt ctgagtcttc gggtgaacag agtgctaaca aaatgttgcc gaacaacaag
    22801 ccaactgcga ccacggtcac agtgcctgta acggtaccaa cagcaagcac aaccgagccg
    22861 actgtcagca gtacatcaac cagtacgcct atctccacct ggcgctggcc gactgagggc
    22921 aaagtgatcg aaacctttgg cgcttctgag gggggcaaca aggggattga tatcgcaggc
    22981 agcaaaggac aggcaattat cgcgaccgca gatggccgcg ttgtttatgc cggtaacgcg
    23041 ctgcgcggct acggtaatct gattatcatc aaacataatg atgattacct gagtgcctac
    23101 gcccataacg acacaatgct ggtccgggaa caacaagaag tgaaggcggg gcaaaaaata
    23161 gcaaccatgg gtagcaccgg aaccagttca acacgcttgc attttgaaat tcgttacaag
    23221 gggaaatccg taaacccgct gcgttatttg ccgcagcgat aaatcggcgg aaccaggctt
    23281 ttgcttgaat gttccgtcaa gggatcacgg gtaggagcca ccttatgagt cagaatacgc
    23341 tgaaagttca tgatttaaat gaagatgcgg aatttgatga gaacggagtt gaggtttttg
    23401 acgaaaaggc cttagtagaa gaggaaccca gtgataacga tttggccgaa gaggaactgt
    23461 tatcgcaggg agccacacag cgtgtgctgg acgcgactca gctttacctt ggtgagattg
    23521 gttattcacc actgttaacg gccgaagaag aagtttattt tgcgcgtcgc gcactgcgtg
    23581 gagatgtcgc ctctcgccgc cggatgatcg agagtaactt gcgtctggtg gtaaaaattg
    23641 cccgccgtta tggcaatcgt ggtctggcgt tgctggacct gatcgaagag ggcaacctgg
    23701 ggctgatccg cgcggtagag aagtttgacc cggaacgtgg tttccgcttc tcaacatacg
    23761 caacctggtg gattcgccag acgatcgaac gggcgattat gaaccaaacc cgtactattc
    23821 gtttgccgat tcacatcgta aaggagctga acgtttacct gcgaaccgca cgtgagttgt
    23881 cccataagct ggaccacgaa ccaagtgcgg aagagatcgc agagcaactg gataagccag
    23941 ttgatgacgt cagccgtatg cttcgtctta acgagcgcat tacctcggta gacaccccgc
    24001 tgggtggtga ttccgaaaaa gcattgctgg acatcctggc cgatgaaaaa gagaatggtc
    24061 cggaagatac cacgcaagat gacgatatga agcagagcat cgtcaaatgg ctgttcgagc
    24121 tgaacgccaa acagcgtgaa gtgctggcac gtcgattcgg tttgctgggg tacgaagcgg
    24181 caacactgga agatgtaggt cgtgaaattg gcctcacccg tgaacgtgtt cgccagattc
    24241 aggttgaagg cctgcgccgt ttgcgcgaaa tcctgcaaac gcaggggctg aatatcgaag
    24301 cgctgttccg cgaataagta agcatctgtc agaaaggcca gtctcaagcg aggctggctt
    24361 tttttctttt ggtacatgtt gaactgttat cggatacagc gttaaaactt tatctgactg
    24421 accaggggac ggcacttttt agatgtagaa gaccggatag acgcgtcagc atcacattcg
    24481 gcaatcatca ttttaagcgc gcagtaattg tcgccacgcg ttcaccatat ttcacggctg
    24541 tatccagatc gcccgtaggg atttgctctg cacccgcatc ggctggagat tgcaccagca
    24601 aaccgaccga accgcccaga ttgtttacat cttccctggt tgacgcaagc acattggcag
    24661 gagcctgacc taaactcacc cacagaccgc catgctgtga agccagcgtt tgcagataga
    24721 tcagcgtaac ttgtttatcg ccattcaggc tggcgctgtt ggtgaaaccc ccgaagactt
    24781 tatcctgcca tttacgtgta aaccacgctt tggaactggc atcagcaaat ttcttgaatt
    24841 gccatggcgc accgcccata taggtgggag caccgaagat aatggcatcg gctgcgtcca
    24901 gtttttccca ctcctgctca gtgatatttc cttcgttatc aatcgaaatt aaatctgcat
    24961 gggcgccttg tgccactttt tcagcaacca gtttagtgtg accatatcct gaataataaa
    25021 ccacagctat gttttgcata taaatatcct ttttactaat gaatgatttt ttgaggcagg
    25081 ttttaaagat gtggagtatt atgccttgca gatattattt aaaccatcaa atgatgaaca
    25141 tattttttga atgagataaa cataatattc gaagaaaaat gaatctcgta acgtagtaat
    25201 atctaaaata acagtctgtt cagagatgct ataaatcaac aacacattaa taaaggacta
    25261 cgtatgcaag gcattaaaat atatacaatt ctagcctctt ctcttatatc tggtagtgtt
    25321 tttgctcaaa cggaaatttc gacttcaaat tcgtctacag ttaatgcaag ttatgttgaa
    25381 ccttcagcgg aaaaaataat atcgccaagc gataaactca ataacctttt tgaaagaaat
    25441 atgtctcagc cttatatttt acaaaaaatt ggcgagagaa cttattatgt tcaacgctat
    25501 ttttattcca ctacgtttta tgtcggagac aaaggggttt tgctgtttga tgctccggag
    25561 ggtcgcggta aatatttatt acaggcaatt cgtgatgtga ctccattacc cgtaacagcg
    25621 ctggtttatt ctcattacca tgtggatcac attggcgata gtccattttg gaacgatgag
    25681 gcgaagaaag aaggcgtgaa tctgcgtatt attgccagta aagccactgc tgaaaaaatg
    25741 caatttatga acagtcgctt acccgtcgcc actcaggttt taagtaaaaa agatgaccag
    25801 tttaaatttg aaaagcagac cattgaatta caccgatttg ttaaagcagg acataccgac
    25861 gaccattctg tatggttgct aaaacaggaa aaagtagctc acagcccgga tctcctgaat
    25921 cctgaccaac tgccgatgat gggcttcgcc gtatcggata cgttggttta ccatgacagt
    25981 aatttgcgtc aggttgaaat gcttgactgg aaatatttta ttggcgggca tggtaatatt
    26041 ggttcacacg atgattttaa atttcaacgc cagtttctta atgatcttcg ggatacgaca
    26101 attaaagtca gaaaagaaga aagttttggt aaatttatga ataaaacggc aaataatcat
    26161 gctgattttg cgcgagccca gcgcgaagca attattaaaa aagtaactga agtattacgt
    26221 ccgaaatatg gccatatgta tggctacgac gcatctatgc ctgctaatat cgaaatggca
    26281 ataaggttgg tcggttctta ttactaagat aaaatcaact tgcaagagac tgtaattata
    26341 ttgcaaagtg aaaattaacc tcggtagcga gatattaccg aggttaatat tggcatacat
    26401 tacaccctta tcaggcactt aaacgataca tcatatctta tctggattat tagatgacga
    26461 gccatacgca ccaggtaatt aagaatccgg taaaaccgag aatggtcgtt aacactgtcc
    26521 aggttttcag accgtctgct accgacaacc ccagatattt ggtcacaatc cagaaccctg
    26581 agtcattaat atgtgacgcg ccaagcccac caaagcaggc tgccagcgtc accaatacgc
    26641 actgaattgg attcaatccc atcaccgctt ctgagagtaa cccgccggtt gtcaggattg
    26701 ctacggttgc tgacccctgc gatgcacgca gcgccagtga aataataaat gcggctggta
    26761 acagaggcag gtcaatcatt tgtaacatgt tggcaagggc tttaccgacg cccgattcca
    26821 ccagcacttt gccaaatacc cctccagcac cagtaaccaa aatcactacc gccgcagtgg
    26881 gaagggcaga gcccataatg tcgctggtgt gttgtaagct ccagccgcga cgtaaagcca
    26941 ataaccagaa tgccagcacc agcgcaatca ttagagctac cattggtgag ccgatcaact
    27001 gtagcgtacc aagcagggga tgcgaaggcg gcatcagtgt tgcagaaacc gttcccgcca
    27061 tgataatcgc gataggaata acaattagcg aggtgactaa cgcgacgccc ggtggattta
    27121 ttttatcgct taattttgtc gcgccttcct cactggccgg agccagttgc atctgttcca
    27181 gtacctcgac cgacatcgca tattggcgct tattgattat tttcgctgca aagtagccaa
    27241 caatccctac gggaatagaa atcgcaatac cgatgatggt tagccagccg atgtctgcgt
    27301 ggagtaatcc cgctgcggcg acagggcctg gatgcggcgg taccgccacg tgaacagtga
    27361 gcatgatccc agcgacaggc aggccaaatt tgagtggtga tattttggca accttggcaa
    27421 aaccgtaaat gattggcgca agaataataa agccgacatc aaagaagacg ggaatgccga
    27481 ggaagaacgc tgccagagtc agcgcagcga tagttcgttt gtcacctaac ttgcgactga
    27541 aataattagc cagtgactct gcaccaccag agtgttcgat catacgcccc agcatagcgc
    27601 caagaccaat aataatagtg acggaaccaa gcacaccgcc catcccggcg atcatcactt
    27661 tacccacttc gcccgccggt atacccgccg caagtgcgac taacaggctg acgaggagca
    27721 gagcaacgaa tggttgtacc tttgccttga tgaccagtag caacagcatg attacgccag
    27781 ctaacgcaat gcataacaat gtaattgtgg acatgggaaa ccctgtctga aagttatagt
    27841 taacctaccc catccgtaga tggggggatg tatgggtacg ttgtaattag ggattgagcg
    27901 aattagcgcc aggcgtcaaa ccagccaagc ccttctttgg tgaggccacg aggtttgtat
    27961 tcacaaccga tccagccctg atatcccact tcatcgaaca ggcggaacag ccacggatag
    28021 ttgatttctc catcgtccgg ttcatgtcga tcaggtagtc cggcaatttg tacgtgcgca
    28081 tatttcccgg cgtagttgcg gattaaatgt gtcaggttgc catctacttt ttgcgcatgg
    28141 aaagtatcta gttgaataaa cacgttatct cgcgcaacct cttcaacaat agccagtgcc
    28201 tgatactggc tggagaagag ataatgaggc ttaacgccgg ggctgagtgc ttcaaccaat
    28261 attcgcttgc cgtgtggcgc aaagcggtcg gcagcgtagc ggagattatc aataaatact
    28321 gcccggtacc gttcagcatc ttcgccagcg ggcacgacgc ctgccatcac atggacttgt
    28381 tcacaattga gcgccaatgc atattccagt gccaggtcga tgtctgcgcg tgcttcgtgc
    28441 tcacgtccgg gaagggcgga taatccccat tctcccgcat taatatctcc gggagcggta
    28501 ttgaacagcg ccagtgtcag atggttttgc accagttgct gctggatttg ctggggggag
    28561 tagtcatagg gaaacagaaa ctccacagca tcgaacccgg cttttcgcgc tgcggcgaag
    28621 cgttcaataa aaggcacttc ggtgaacatc atggataaat tagctgcaaa acgaggcatt
    28681 gcattaactc cttaattccg caatttcacc tgcggtcaga taacggatcg ggcggtcacc
    28741 gagaataaaa atcagctttg ccgtttcctc cagctcttcc atattgttgg cggcttcttg
    28801 caggctttca ccgcaaacca ctgggccatg atttgccagt aaaaaagcct gattgtctgc
    28861 tgccagttcc gccagatcct gtgcgatgcg tttatcgccc ggtcggtaat agggcaccag
    28921 cgggacgttt cccatccgca tcaccacgta tggtgtgaac ggacgaataa cgttattgct
    28981 gtccagccct tccaggcagg aaagcgccgt cgaccacgta ctgtgcaaat gcaccaccgc
    29041 tttacagagc ggattgttgc gatacagcgc cagatgaaag agcacctctt tcgaggggtt
    29101 gtcaccactt aaccattcgc catccgcggt gactttggaa agccgctgcg gatcgagatt
    29161 gcccaggcat gaacctgtcg gtgtcgccag taaattcccg tcaggtaaaa gcagcgacag
    29221 attgccagcc gaaccggttg catatccgcg ctgaaagaat gaactggcaa tccgcgtcat
    29281 ctcctctcgc aaagactgct ctacttttgc gaaatcgctc atgataaaaa ctctctttgg
    29341 gctcgtgaaa aaaaggcttc atcaccgaag ttgccagatt taagggcgag tgagacaggc
    29401 ttatccagtg cgtttaccca cggcacgccg ggggaaatgg ttgggccaat atgaaaccct
    29461 tttatgccca ggctctgtgt gactacgccg gaggtctcac cgcctgcgac aataaagcgt
    29521 gtcacgcctt ccgctgctaa ccgcgccgct agttgagaaa acagagtttc tactgcctga
    29581 ctggcttttt gtgcaccgta ttgctgttga attgctgcca atgcgtcagt gctggcggtg
    29641 gcaaaaacca gtggagcaag tacactttcc tggcccagaa cccactctgc cagctcgtgt
    29701 gcataagcgg ccagagtttc ggttgagagg cagcgtgcca catcaacctc acgggctggt
    29761 gcaatttgac ggtaatgtgc cacctggcgg ttggtcattt gagagcatga accggagagc
    29821 actacgccgc gcccagcgag cggacgccct gcttcgcgag cctggttacc gttttcttgc
    29881 gcccactgcc gggccaggcc aatcgccaga ccagaaccgc ccgttaccag tggggcatcg
    29941 cgcaaggctt ccccctgaat ttccagatgg tgttcggtca gcgcatcaag caccgcgtag
    30001 cgatagccct cttgctgtaa gcgagccagc tcctgacgaa cggctttcac accttgttcg
    30061 aaaacatgtg ctgaaacgac gccgcagcgc cctgtggatt gcgcttcaac cagacgggga
    30121 agatagctgt cggtcatggg atttaccggg tgatggcgca ttccggattc ggccagcagt
    30181 tgattcatta cgaacaaata cccctgataa accgtacgtc cgttgactgg cagggccgga
    30241 gagaagaccg taaacggtgt gtcgagagca tccattaatg catcggtaac tgggccgata
    30301 ttccctttcg ccgtactgtc gaaagtagag cagtatttga aatagatctg tttgcaacct
    30361 tgctgttgca accagctcag agccgccagc gattgctgtg tggcttcaac caccggacag
    30421 gagcgcgttt tcaggctaat caccagtgcg tcgattgctt ccggcatttt acctgttgga
    30481 acaccgttaa tttgtaccgt tggtagaccg ttttccacca gaaaactggc gatatccgtc
    30541 gcgccggtaa aatcatcagc gataacgcca atcttgatca tgatttcgct cccggtaggg
    30601 tgatgccaga gaaaatcttg ataactgcgc tatcgtcttc tttcccgtaa cccgcgttac
    30661 tggcgctggt gaacatattc aatgctgttg aggccaatgg cagcgggaag tgcagggctt
    30721 tggcggtatc ggcaaccaga ccaagatcct taacaaaaat atcgacggct gaatgcgggg
    30781 tgtaatcgcc atccaccaca tgacgcatcc ggttttcgaa catccatgaa tttccggcgg
    30841 cattggtcac gacgtcatac atcacatcca gcgggatccc cgcacgggct gcaagcgcca
    30901 tcgcttcggc tccggcagca atatgtacgc ccgccaacaa ctggtggata atttttacgg
    30961 tcgaacccag tcccggttct gctccgatgc gataaacttt tccggcaacg gcttccagca
    31021 cgggtgccag tcgttcaaag gcaatatcgc taccggaggc catgacagtc atttcaccgt
    31081 tagcggcttt taccgcacca ccagaaaccg gcgcatccag catttccaga ccgaatccag
    31141 ccagagcggt agcaatttct tgcgcatcag cattggcgat agtggaagaa accattactg
    31201 ccgtaccggg tttcagatgt tgtgcaacgc ctttttcacc aaacagcacc tgtttaacct
    31261 gggtcgcatt gaccaccagc accagcagag cgtccagttt ttcggcaaac gtcgcggcgt
    31321 tatcagaaac cccgcaagca ccagcctctt tcaacgtagc gcaggcattg ctgttcaggt
    31381 ctgcgcccca ggtagaaaga cctgcgcgga cacatgacag tgctgctccc attcccattg
    31441 accctaagcc aacgataccg acatgaaact ccgatcccgt tttcatctgc tctccttgtt
    31501 aatttaagtg atattttgtt tgatattgtg aatataagcg cggaaagata acgatatggt
    31561 gagctgattc acaaaaatta acattgtgtg ttattttatg tgaactaagc gttagttgcg
    31621 ccgcgcacgt ttcgcaggca aatagcgtag aatgtcagca ggacaaagga aggagcaaaa
    31681 gttgataccc gtagagcgtc gccaaatcat ccttgagatg gtagctgaaa aaggcattgt
    31741 tagtattgct gaactaacgg acagaatgaa tgtgtcacat atgaccattc gtcgggattt
    31801 acaaaaactg gagcagcagg gagccgttgt gctggtgtcc gggggcgtcc agtctccggg
    31861 acgcgtggcg catgaacctt ctcatcaggt aaaaactgcg ctggcaatga cgcaaaaagc
    31921 ggctattggc aagctggcgg caagtcttgt tcagccggga cgttgtatct atctggatgc
    31981 gggaacgacc acgttagcga ttgcacagca tctgattcac atggagccac tcacagtggt
    32041 tactaacgac ttcgttattg cggactactt gctcgacaac agtaattgca caattattca
    32101 cactggcggc gcagtgtgtc gggaaaaccg ttcctgtgtt ggggaagccg ctgcgaccat
    32161 gctgcgtagc ctgatgattg atcaggcttt tatttctgca tcgtcatgga gtgtgcgggg
    32221 gatctctacg ccagcagaag ataaagtcac ggtgaaacgg gcgattgcca gtgccagccg
    32281 ccagcgagtt ttggtctgtg atgcgacgaa atatggacag gtggcgacat ggctggcgtt
    32341 accattaagc gagtttgatc agatcattac tgacgacggt ctgccggaga gtgccagccg
    32401 cgcgctggcg aagctggatc tctctttgct ggtagcgaaa aatgaataat gacctgcaat
    32461 aacgccgggt tactcatgct tcacagaaga agcatgagac tactttattt tataaaatga
    32521 cagccgcccg cttttcggcg atccggtatc tatataaatc tggttagcga acgtctgaat
    32581 gttatcaaac atcatatgac caaatataaa gaagtcggcg ccgtttattt tttgtaattc
    32641 gctattaagt gatttctgca cacgatcaac aggccagagt aattcgctct ccgctatttc
    32701 tttgccaaac tgatactcct tccccggata atctgcatgt gcgatgacat attttatgtt
    32761 gtcgttagtg atttcaataa tatgtggaag gagatggaat ttcagcaaca gatctattgc
    32821 ctcttgttgc tctgaatcat ttaaatcgaa aaaccagtca ccaccgctgg caagccacat
    32881 atttccatcg ccagtttcga atgcctcaag cgccatcgct tcgtggttgc ctttgacgga
    32941 gataaaccag ggctggttta acaggcgcag cacgttaaga ctctccggcc cacgatcgat
    33001 gttatcgcct accgaaataa gtaagtcggt ttcaggacaa aaagagagtt gatgtaagcg
    33061 ggattgtaat aattgatagt caccatgaat atcaccaacg gcccatatat ggcgatagtg
    33121 atgggcattg attttttgat agcgtgtaga tggcatggtt ttaccctgta aaataagttt
    33181 tcctattata cagggtattt ttatttgatt cgtcagttgt cgttaatatt cccgatagca
    33241 aaaggctatc gggaattatt attacaccag actcttcaag cgataaatcc attccagcgc
    33301 ctgacgcgga gtcagtgaat ccgggtcgag gttttccagt gcctcgaccg caggcgaagt
    33361 ttcttccggt acggacagca aagacatttg tgtaccatcc acttgcgtag cagcggcgtt
    33421 cggcgaaatg ctttccaact cacgcagttt ttgccgtgcg cgcttaataa cctcttttgg
    33481 cacacccgcc agagctgcaa ccgccaggcc gtagcttttg ctcgccgcgc catcctgcac
    33541 gctatgcata aaggcaatgg tgtcgccgtg ctccagtgca tcgagatgca cgttggcgac
    33601 gccttccatt ttctccggta actgggtcag ctcgaaatag tgggtagcaa acagcgtcaa
    33661 cgctttgatc ttatttgcca gattttccgc acacgcccac gccagcgaca gaccatcgta
    33721 agtggacgtt ccacgcccga tctcatccat caacaccaga ctatattcgg tggcgttatg
    33781 taaaatattg gcggtttcgg tcatctccac cataaaggtt gaacgcccgg aagccagatc
    33841 atccgcagca cctacgcggg taaagatacg gtcaatcggg ccaatctcga ctttttgcgc
    33901 cggtacgtag ctgccgatat acgccatcag cgcaatcaac gcggtctggc gcatataggt
    33961 acttttaccg cccatgttcg gaccggtaat gatcaacatg cgacgctgcg gcgacaggtt
    34021 cagcgggtta gcgataaacg gctcattcag tacttgttca actaccggat ggcgaccttc
    34081 ggtaatacga atgccaggtt tatcaatgaa ggtcgggcag gtatagttca gggtataggc
    34141 ccgttccgcc aggttcacca gcacgtcgag ttccgccagc gcgctcgcgc tctgttgcaa
    34201 cgcttccaga tgcggcaaca gcaggtcgaa cagctcttca taaagttgtt tttccagagc
    34261 cagtgctttg ccttttgagg tgagaacttt atcttcgtac tcttttagct ctggaatgat
    34321 gtagcgctcg gcgtttttca gcgtctggcg acgcatgtag ttgattggtg ccaaatggct
    34381 ttgcccacgg ctgatttgaa tgtagtagcc gtgcaccgca ttaaagccaa ctttcagcgt
    34441 gtccaggccg gtacgttcac gctcgcggac ttccagacgc tccagataat cggtcgcgcc
    34501 gtcagccagc gcgcgccact catccagctc ttcgttatag cccgatgcga taacaccacc
    34561 gtcgcgtacc agcaccggtg gtgtgtcgat gattgctcgc tccagcagat cgcgcagctc
    34621 ggcaaactcg cccatcttct cacgcagcgc ttgtaccggt gcactatcga cattttctaa
    34681 ctgcgcacgc aactccggca gttgctggaa agcgtgacgc atacgggcca gatcgcgtgg
    34741 gcgagcggta cgcaacgcca gacgcgccag aatacgttcc aggtcgccca cctgacgcag
    34801 taccggctgc agctcggcgg tgaaatcctg caatgcgcca atagtttgct gacgctcaag
    34861 caacacgcgg gtatcgcgca ctggcatatg cagccagcgt ttcaacatac gactacccat
    34921 cggcgttaca gtgcagtcga gcactgaagc cagcgtattt tccgcaccgc cggccaggtt
    34981 ctgagtaatt tccaggttac gacgcgtcgc ggcatccata atgatgctgt cctgctgacg
    35041 ttccatagtg atagaacgga tatgcggcag ggtcgtgcgt tgggtatctt tcgcatactg
    35101 caacagacaa ccggcagcac aaagtccgcg tggtgcgttc tccacaccaa aaccgaccag
    35161 atctcgggtg ccaaattgca ggttcaattg ctggcgagcg gtgtcgattt caaactccca
    35221 cagcgggcga cgacgcaggc cgcggcgacc ttcaatcagc gacatctcgg cgaaatcttc
    35281 tgcatacagt aattccgctg gattcgtacg ttgcagttct gccgccatcg tttcgcggtc
    35341 cgccggttcg ctcaggcgaa aacgaccgga actgatatcc agcgttgcgt agccgaaacc
    35401 tttactgtcc tgccagatag ccgccagcag gttgtcctga cgttcctgca acagcgcttc
    35461 atcgctgatg gtgcccggcg taacgatacg gacaactttg cgctcgaccg gccctttgct
    35521 ggtcgccgga tcgccaatct gttcacaaat agcgacggat tcgccctgat tcaccagttt
    35581 ggcgaggtag ttttccaccg catggtaggg aatccccgcc atcgggattg gctctcccgc
    35641 tgaagcaccg cgtttggtca gtgaaatatc cagcagttgc gacgcccgtt ttgcgtcgtc
    35701 ataaaacagt tcataaaagt cgcccatccg gtaaaacagc aggatctcgg gatgctgggc
    35761 tttcagcttg agatactgct gcatcatggg cgtatgggcg tcgaaatttt ctattgtact
    35821 catggggtta agtccggctc cctgatgtta aatgtagtgt gatggttttt attttcgttg
    35881 taatcacata aggcgcacat gataccggag ttaatcattt caggggaaga cgcgaaaggt
    35941 gagttcttga tgtcggttcc gcgaaaaatg ctgctttgca gcttttgcgt gatgacgatg
    36001 aaacaagatc gccaaattcg tcggttgaag aaacctcctc cttcagagcc atagtcccct
    36061 ttaaaattaa gttgtaatat aaatgcacct taagagtatg gaggcagcat gtccggcaag
    36121 cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg atctttatca agcgaaatgc
    36181 ccgcaggcgt cagcagagcc ggagcattac gaggcgttgt ttgcttacgc gcaaaagcgg
    36241 ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta agcagtgtcc ggtgcactgt
    36301 taccagcctg caaagcgtga agagatgaaa cagattatgc gttgggccgg gccgagaatg
    36361 ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg atgacaagcg tccggtgccg
    36421 gagttgccag aaaaatatcg tccaaagaaa taaatctgcc ggagagccat actctccggc
    36481 aaatacggcg cttacgcttt ggtggcaatc aggatggctg atgctttgat caatgcaata
    36541 acgcgcgagc cagtggtgag tttcatttca tcctgacttt catttgttac cactgccaca
    36601 atttcaaaac ccgcatccgt tttgatatgg acagtggcgt tcactgcgcc ttcggtgatt
    36661 gtcgacacgc tgccagcaaa ctggttgcga gcagagaatt tcagtccaca gtcttccgtt
    36721 gccagcgtta cccacggggc tttaatcagg gcaatcgctt ctttcccttt cgccagaccc
    36781 agcgcttgct ggctgctgtg agtcacaatt gccaccagct ttgcaccacc tgccagcgtc
    36841 agttctactt cgtcattcac cgcgcccatt gctactgcgc taactgttcc ggttaattga
    36901 tttcttgctg aaactgccat actgcctcct ttcattgaat gttgatcgag tataaccaca
    36961 tctgttaaat caatgccgat ttatcaattc ccagtcgctt cattcgtgac agcagagtcg
    37021 tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg ccgtttgttt
    37081 ctttcagcac gcgaacaatc aactgatatt catcttcgcc ctcctgggcg acaaccgttg
    37141 cggcaggcgg cgtttcaggt tctggtaaag caatatctgg caatgacagc tgcagcacgt
    37201 tgccgcgtgt tagcaatacc gcgcgctcga tgacgttttc cagctcacgc acgttgcccg
    37261 gccattccat gttgctcagg atgcgcagcg tctcggcagg aatgctgtcg atattgcgcc
    37321 ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata tcttccggac
    37381 gctcgcgtag tggcggcagg tggatcggga atacgttcag gcggtaatag agatcgctac
    37441 ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca attaaacgta
    37501 cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc tgtaatacac
    37561 gcagcaactt cggctgtaac tccagcggca tatcgcccac ttcatcgagg aatagtgagc
    37621 ttttatccgc cagttcaaaa cgaccaatgc gctgagcgct ggcaccggta aaagccccgc
    37681 gctcatgacc aaatagatcg ctttccagca atccggcagg catcgccgcg cagttcattt
    37741 tgaccatgcg gcgattatta cgcccactga gattatggat cgcgcgggca atcagctctt
    37801 tacctgtacc agtttcaccg aggatcagaa cggtactgtc actttgcgcc accatttcaa
    37861 cttgtttaag tacgctgtac atggcttcgc tgcggccaat aatctcgcca aattcactat
    37921 caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt tctttcagac
    37981 gatgaatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt tcggcaatct
    38041 ggcgcagtaa attcagattg gtagtggtaa agactttctc ttcgcattgc gccagtttca
    38101 gtacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt tgaatctgat
    38161 tgccccaggt gttgaacaac atgcgttcat agggggctaa atcgtcccgc tcgtggagat
    38221 taatcaacag catctcttta cttttgaaga cgcgttcggt gagggttccg gcttcatcga
    38281 cttcgctctg ttcgtgagcg ggatgctgtt tatcaagata gtgagtggag tagatgttga
    38341 gtttgttctt acggtggctg cgtaagacga tgctgatatc gtcaatgtcg aagtagtagt
    38401 ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa agcaccgcgt
    38461 tagtgatggc gaccaggatg cggaagttat cgcgttcccg gcataacaac tcatagtcga
    38521 cattgttaac gacgcggctc tggatttgtt cggtgacgac agaaacgatc tgcgtaaatg
    38581 tttgcagacg attgaactct ttttcgctcc aggggcgatc gtcataacga ataaattcac
    38641 agccaccaaa aatatgccct tccgccgcca gtggcatcag gcaatagtga ccaaatttgg
    38701 ggtatagccc acctgtggcc agctgcggcc aggtttcaca aaattcttcg taactgcaat
    38761 gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga acagtttcgt
    38821 cttcatattt aatgggggtg tctttttcac gcgatgcgta ataagacgca cgttgagtct
    38881 gcgcttgcca caacacaatc gccgcgttgt cggcgagcgc agaacgcttt accagttgcg
    38941 aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga gtgatgtcga
    39001 acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc ttccaggaaa
    39061 gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga attttattag
    39121 catatacgcg gaagcggttc ggcgtgcggt agatcgaggg ttcgtttcac gccatacaga
    39181 ccggcaagac gaacaccttt acgttctact acttcgccaa tcagcgccgc atcttttccc
    39241 aatggatggg aatgtaacgc cgccagcact tgctctgctg cgttgcgttc aacggcgatc
    39301 accagtttgc cttcgttggc aaagttaagg gcgtccagtc ccagcagttc gcaaacgccg
    39361 cgcacagcag gtttaaccgg cagtgccgct tctgaaagtt caataccaca accgcaggct
    39421 gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg cagcgctttc
    39481 acgccgggaa tgtcacgcag cgtctgaata agcggcgcca gcaccgcgca gtcgctgacc
    39541 agttctccat ccagccctag ttgctcacgc aggttaagga tagtcgcccc atggtcgccg
    39601 agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc ccagtgaata
    39661 ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc cgcgccgcgc
    39721 tgtaccactt tggtgtcgcc agtaacgatg gcaatgcctg ccgcacgggc ggtttctgcc
    39781 atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag gataaagcca
    39841 caggagagat agcgcggaat tgcgccactg accgcaacgt cattggcggt gccgcaaatc
    39901 gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta actgtcggtg
    39961 gagaacgcca gacggtcgcc ttccgctacc agctgtgcca aatcaagacg ggcctgatct
    40021 tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt gattaattgc
    40081 tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct tcactctcct
    40141 gctgacgata ctgataccac gcggcgcacg ctccttcgga ggaaaccatc agcgcaccaa
    40201 acgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc ttacatttgc
    40261 ccgtcaagac ttcgccacaa cgcgcacgcg ggtcatcgca gacctgctgc ggtgccgggc
    40321 ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg ccagaagatt
    40381 caatcacgcc taagccgcgc cattcactgt cgccgttgac acagaacaca tcagcaatgg
    40441 cctgttgcgc cagcaggtta ccggcatccg gtaccacccg acgatactga ttctctacct
    40501 tgctgtgggc cgctattttc tgctccacca gcatcaccac gccttgaagt agatcaaggg
    40561 gttcgaaacc agcgaccact agcggacgat gaaaatcgct ggcgataaaa ttataggcat
    40621 cagtgccgat aaccatactg acgtggcccg gcgcgaggaa cgcgtcgata ccgttatctg
    40681 gctgttccag caaactgcgc agtgtcggga taagcgtaat gtgctggcag aagaagtaaa
    40741 aattctgcac atcgcgggct ttcgcctgtt gcagagtgat ggcggtggtc ggcatggtgg
    40801 tttcaaaacc taagccgaag aataccactt tacgggttgg attctcctgc gccagtttca
    40861 acgcatccat tggcgagtaa acgatgcgca catcggcacc gcgtgctttt gcctgcaaca
    40921 gcgacccctg tttccctggt acgcgcatgg catcgccaaa ggtacagaag atgacttccg
    40981 gatggctggc aatctccacg caggtgtcga ttctacccat tggcagtacg cacaccgggc
    41041 aacccggacc gtggataaac tcaacgtttt ccggtagcaa ctggtcgagg ccgaatttaa
    41101 aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt tcggcggtgt
    41161 aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcattacc tgttccggcg
    41221 cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg cgccgacatc
    41281 tggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt cggcttcatt
    41341 aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc gtggctgacc
    41401 gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgttgaatgc cgcagacgtc
    41461 gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta tgcacatcgc
    41521 tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc ggaaataagg
    41581 atgatttcaa tttctggatt gacttcacgg gcgcaggcga tgcacttctc aacgtcaaag
    41641 ttgagatacg gcagcaagtc aactttgttg agcagcatca gcgaggcggc ggcaaacatg
    41701 tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc cactttgtgt
    41761 ttttcaccga gatcgaagct ggccgggcaa acgaggttac cgacgttttc gataaacaga
    41821 ataccgttat cgtccagtgg caggcgcggc gcggcgtcgg caatcatctg tgcgtcaaga
    41881 tggcagcctt taccggtgtt cacctgaatc gctggggtgc cggtagcgcg aatgcgtgcg
    41941 gcatcgttca cggtttgctg gtcaccttca ataaccgcgc acggaacgct gtctttcagg
    42001 cgcattaaag tttccgtcag cagggtggtt ttaccggaac cagggctgga aaccaggttg
    42061 agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag acggttattt
    42121 ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc cggcgcgtga
    42181 gtgcccgctt cgccatgacc gtaatgcaga tcgccttctt cagtctggct gggggtaaat
    42241 tcaggcgctt taatgccggt gattttcatc ttcgggcgtg ccgccggggc aaatggcgcg
    42301 ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatagaggtt gccttcaccg
    42361 caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg aatctgtaaa
    42421 ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg gcggacgcgc
    42481 tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc ctcttgttct
    42541 tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc aaaacaaaag
    42601 gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac cccagttacg
    42661 cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg ttggcagagg
    42721 gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat aattgtgcca
    42781 gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg tcgacacggc
    42841 gacgaaattc atctttagct tagaaatcgc tttaataaca atgaattaaa agttggcaca
    42901 gaaaatgctt aaagctggca tctctgttaa acgggtaacc tgacaatgac tatttgggaa
    42961 ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga ccagtggcac
    43021 atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct gcatcacgcc
    43081 atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt tcgcatttac
    43141 gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga aacaaaagat
    43201 ggcgaagaca aacagcggat tgcgcaggcg cagttgagca ttgacggcat gattgatggc
    43261 aaggtcaaca tccgcgatcg cgaacaagtt ctggaacact atctcgaaaa aatcgctggc
    43321 gtttacgaca gcttatatac cgccattgaa aacaatgtgc cggtgaattt aagccaactg
    43381 gtaaagggac aaagcccggt agcatgagct gaggctttgc ccgttttgca ggcgttacgc
    43441 ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg catgttttcg
    43501 tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg ctgactccac
    43561 gctctgtatc ggctgccaca cttgtgaggc tgcctgttca gagacgcatc gccagcacgg
    43621 cctgcaatca atgccgcgcc tgcgagtgat gctcaatgaa aaagaatctg cgccgcagct
    43681 ctgtcaccac tgtgaagatg caccctgcgc aacggtctgc ccggttaacg ccatcactcg
    43741 tgttgatggt gccgtacagc tgaatgaaag cttgtgcgta agctgcaaac tgtgtggcat
    43801 cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata ttccggcaaa
    43861 caccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca gcacattgct
    43921 tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta gctttgatga
    43981 acaaggtccg gcctgcgtgc ggatgtgccc gactaaagcc ctgcatctgg tggataacac
    44041 cgatatcgcc cgcgtcagca aacgtaagcg tgagctgact tttaacacgg actttggcga
    44101 tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat ttccctgatc
    44161 aatagcggcg tggcgtggtt tgtcgccgcc gctgttctgg catttctctt ttcttttcaa
    44221 aaagcgctaa gtggctggat agccggaatt ggcggcgcgg tgggtagcct gtatacggca
    44281 gccgcgggct tcactgtgct gactggcgcg gttggcgtga gcggtgcgct gtcgttggta
    44341 agctacgatg tgcaaatctc tccgcttaac gcgatttggc tgattacgct cggtctgtgc
    44401 ggtctgtttg tcagcctcta caacattgac tggcatcgcc acgcgcaggt gaagtgcaac
    44461 ggcttgcaga tcaatatgtt gatggctgcc gccgtctgtg ccgtcatcgc cagcaacctc
    44521 ggcatgttcg tggtaatggc cgaaatcatg gccctgtgtg cggtgttcct caccagcaac
    44581 agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg gcactctgct gctggcgatt
    44641 gcctgctggc tgctgtggca gcgttacggc acgctggatc tgcgcctgct ggatatgcgt
    44701 atgcaacagc tgccgctcgg ttccgatatc tggctgctcg gtgtgattgg ctttggcctg
    44761 ctggccggga ttattccgct gcacggctgg gtgccacagg cacatgccaa cgcctctgcg
    44821 ccagctgccg cgctgttttc cacggtagtc atgaaaattg gcctgctggg cattttaact
    44881 ctgtcactac tgggcggtaa tgcaccgctg tggtggggga tcgcactgct ggtactcggc
    44941 atgatcaccg cattcgtcgg cggtctgtat gcgttgatgg aacataatat ccaacgtctg
    45001 ctggcttacc acaccctgga aaatatcggc atcatcctgc tggggctggg cgctggcgta
    45061 acgggtatcg cgctcgaaca accggcgctg attgcccttg gtctggttgg tggtctgtac
    45121 catctgctta accatagcct gttcaagagc gtactgttcc ttggtgcggg gagcgtctgg
    45181 ttccgtaccg gtcatcgcga tatcgaaaaa ctcggtggta ttggcaagaa aatgccggtt
    45241 atctccatcg ccatgttagt cgggctgatg gcaatggctg cgctgccgcc gctgaacggt
    45301 tttgccgggg aatgggttat ctatcaatcc ttcttcaaac tgagcaatag tggcgcgttt
    45361 gttgcccgtc ttctcgggcc gctgctcgcc gtggggctgg caattaccgg tgcgctggcg
    45421 gtgatgtgta tggcgaaagt ttatggcgtt actttcctcg gcgcgccgcg taccaaagaa
    45481 gccgaaaacg ccacctgttc gccgctcctg atgagcgtaa gcgtagtggc actggcgatt
    45541 tgctgcgtaa ttggcggtgt tgctgcgccg tggctactgc cgatgctctc tgctgctgta
    45601 cctctgccgc tggagcctgc taacaccacc gtttctcaac caatgatcac gttgttgttg
    45661 attgcctgcc cgctgctgcc attcatcatt atggcgattt gcaaaggcga tcgtttgccg
    45721 tcgcgttccc gcggcgcggc ctgggtgtgc ggctacgatc atgaaaaatc aatggtgatt
    45781 accgctcacg gttttgccat gccggtgaaa caggcgtttg cgccggtgct gaaactacgc
    45841 aaatggctga atccggtgtc tctggtgccg ggctggcagt gcgaggggag tgcgttgctg
    45901 ttccgccgga tggcgctggt tgaactggcg gtgctggtgg tgattattgt ttcacgagga
    45961 gcctgagaat gagtgtttta tatccgttaa ttcaggcgct ggtgctattt gccgttgcgc
    46021 cgctgctctc cggtattacc cgcgtagcac gcgcccgtct gcataaccgt cgtgggccgg
    46081 gtgtgttgca ggagtatcgc gacattatca aactgctggg ccgccagagc gttggcccgg
    46141 atgcctccgg ctgggtgttc cgcctgacgc cgtatgtgat ggtgggcgtc atgctgacta
    46201 tcgccactgc gctgccggtg gtgaccgtcg gttctccgct gccgcaactg ggcgatttga
    46261 tcaccttact gtatctcttc gccatcgcgc gtttcttctt tgccatttct ggtctggata
    46321 ccggtagccc gtttaccgct atcggcgcga gccgtgaagc gatgcttggc gtgttggttg
    46381 aaccgatgct gctgcttggt ctgtgggtcg ccgcacaggt tgccggttcc accaacatca
    46441 gcaacatcac cgacaccgtt tatcactggc cactgagcca gagcatcccg ctggtgctgg
    46501 cgctttgtgc ctgtgcattc gccaccttta tcgaaatggg caaactgccg ttcgacctgg
    46561 cggaagccga gcaggagttg caggaaggtc cgctctctga gtatagcggc agcggctttg
    46621 gcgtcatgaa atggggtatc agcctgaaac agctggtggt gttgcagatg ttcgtcgggg
    46681 tgtttattcc gtggggacaa atggaaacct tcaccgtcgg tgggctgctg ctggcgctgg
    46741 tgattgccat cgtcaaactg gtggttggcg tcctggttat cgcgctgttc gaaaacagca
    46801 tggcccgtct gcgtcttgat attactccgc gcattacctg ggccgggttt ggctttgcat
    46861 ttttagcgtt cgtctccttg ctggcggcgt gattaaagag agtttgagca tgtctgaaga
    46921 aaaattaggt caacattatc tcgccgcgct gaatgaggca tttcctggcg tcgtgctgga
    46981 ccacgcctgg cagaccaaag atcagctgac cgtgaccgta aaggtgaact acctgccgga
    47041 agtggtggag tttctctact acaagcaggg tggctggctg tcggtgctgt ttggtaacga
    47101 cgaacgcaaa ctgaatggtc attacgccgt ttattacgtg ctgtcgatgg agaagggcac
    47161 caagtgttgg gtaacggttc gcgttgaagt tgacgccaac aaaccggagt atccttccgt
    47221 gacgccgcgc gttccggcgg cggtgtgggg cgagcgtgaa gtgcgcgata tgtacggttt
    47281 gattccggtt ggtctgccgg atgaacgccg tctggtgctg ccggatgact ggccggatga
    47341 actttatcct ctgcgtaaag acagcatgga ttatcgtcag cgtccggcac cgactaccga
    47401 tgctgaaacc tacgagttca tcaacgaact gggcgacaag aaaaacaacg tcgtgccgat
    47461 tggtccactg cacgtcactt ctgacgaacc gggtcacttc cgtctgttcg tcgatggtga
    47521 aaacattatc gacgccgact accgcctgtt ctacgtccat cgcggcatgg aaaaactggc
    47581 ggaaacccgt atgggttaca acgaagtgac cttcctctct gaccgtgtgt gcgggatctg
    47641 cggctttgct cacagcaccg cctataccac gtcggtggaa aacgcgatgg gtattcaggt
    47701 gccagaacgc gcgcagatga tccgcgccat tctgctggag gtagaacgcc tgcactcgca
    47761 tctgctcaac ctcggcctgg cctgtcactt taccggcttt gactccggct ttatgcagtt
    47821 cttccgcgtg cgtgaaacct ccatgaaaat ggcagagatc cttaccggtg cgcgtaaaac
    47881 ctacggcctg aacctgatcg gcgggattcg tcgcgatctg ctgaaagacg acatgattca
    47941 gacccgccag ctggcgcaac agatgcgtcg tgaagtgcag gagctggtgg atgtgctgct
    48001 gagcacaccg aacatggaac agcgcaccgt cggcattggt cgtctggacc cggaaattgc
    48061 ccgcgatttc agtaacgttg gcccgatggt ccgcgccagc ggacacgctc gcgatacccg
    48121 cgccgatcac ccgtttgttg gttatggcct gctgccaatg gaagtccaca gcgagcaggg
    48181 ctgcgacgtt atttcccgtc tgaaagtgcg tatcaacgaa gtctataccg cgctgaatat
    48241 gatcgattac ggtctggata acctgccggg cggcccgctg atggtggaag gctttaccta
    48301 cattccgcac cgtttcgcgc tgggctttgc cgaagcgccg cgcggcgacg atatccactg
    48361 gagcatgacc ggcgacaacc agaagctgta ccgctggcgc tgccgtgccg cgacctacgc
    48421 gaactggccg accctgcgct acatgctgcg tggcaatacc gtttccgatg cgccgctgat
    48481 tatcggtagc ctggaccctt gctactcctg taccgaccgc atgaccgtgg tcgatgtacg
    48541 taagaagaag agcaaagtgg tgccgtacaa agaactcgag cgctacagca ttgagcgtaa
    48601 aaactcgccg ctgaaataag gaatcgccat gtttaccttt atcaaaaaag tcatcaaaac
    48661 cggcacggcg acttcgtctt atccgctgga gccgattgcg gttgataaaa acttccgtgg
    48721 taagccagag cagaacccgc agcagtgcat cggctgtgcg gcctgcgtca atgcctgccc
    48781 gtcaaacgcc ttaacggttg aaactgacct cgccacagga gagctggcgt ggcagttcaa
    48841 tcttggacgc tgcatcttct gtggacgctg cgaagaagtc tgcccgacgg cggcgattaa
    48901 gctgtcgcaa gaatacgaac tggcggtgtg gaagaaagaa gacttcctgc aacagtcccg
    48961 cttcgcgctg tgcaactgcc gcgtctgcaa tcgtcctttc gccgtccaga aagagatcga
    49021 ctacgccatt gcgctgctta agcacaacgg cgacagccgc gcggaaaacc accgcgaaag
    49081 ctttgagact tgcccggaat gtaagcgcca gaaatgcctg gtgccgtccg accgtattga
    49141 actgactcgc catatgaaag aggccatctg atgagcaatt tattaggccc ccgtgacgcc
    49201 aacggcattc cggtcccgat gacggtggat gaatccatcg ccagcatgaa ggcgtcgtta
    49261 ctgaaaaaaa tcaaacgttc tgcctacgtt tatcgcgtgg actgcggcgg ctgtaacggt
    49321 tgcgaaatcg aaattttcgc cacgctttcg ccgctgttcg atgcagaacg cttcggcatt
    49381 aaagtcgttc cgtcaccgcg tcatgcggat attttactgt ttaccggcgc ggtcacccgt
    49441 gcaatgcgat cccctgcgct gcgtgcgtgg cagtccgcgc cggacccgaa aatctgtatc
    49501 tcttacggtg cctgcggtaa cagcggcggg atcttccacg atctctactg cgtgtggggc
    49561 ggtacggata aaatcgttcc ggtagatgtc tacatccctg gctgcccgcc aacgcctgcc
    49621 gccacgctgt acggctttgc gatggcgctc ggcctgctgg agcagaaaat ccacgcccgt
    49681 gggccgggtg agcaagatga gcagccggca gacattctac atcctgatat ggtgcaaccg
    49741 ttgcgtgtga aagtggaccg tgaagcgcgt cgtctggcgg gttatcgtta cggtcgccag
    49801 attgccgatg atttcctgac acagttaggg cagggcgaag aacaggttgc acgctggctg
    49861 gaagcggaaa acgatccgcg tctgaacgag attgtcagcc atctgaatca tgttgttgaa
    49921 gaggcgcgta tccgatgagt gaaaaggtgg tgttcagtca actgagccgt aaatttattg
    49981 atgagaacga tgccacgccc gccgaggcgc agcaggtggt ctattacagc ctggcgattg
    50041 gtcaccacct tggggttatc gattgcctgg aagcggcact tacctgcccg tgggatgact
    50101 atctggcatg gattgccact ctggaggcgg gcagcgaagc ccgccgtaaa atggaaggcg
    50161 tgccgaaata tggtgagatc gtcatcgaca ttaaccatgt gccgatgctg gccaacgcat
    50221 tcgataaagc ccgggcagcg caaacttcgc agcagaagga atggagtaca atgctgttaa
    50281 gtatgctgca tgatattcat caggaaaacg ccatctattt gatggtgagg agactgcgtg
    50341 actgacgttt tactttgtgt tggcaatagc atgatgggcg atgatggcgc aggcccgcta
    50401 ctggcggaaa agtgcgccgc cgcgccgaaa ggtaactggg tagtgattga cggcggtagc
    50461 gcaccggaaa acgacattgt cgctatccgc gaactgcgcc cgacacggct gctgattgtc
    50521 gacgccacgg atatggggct aaaccctggc gagatccgca tcatcgaccc ggctgacatc
    50581 gccgagatgt ttatgatgac tacccataac atgccgctga actatcttat cgaccagttg
    50641 aaagaagata ttggcgaagt gattttcctt ggcatccagc cggatatcgt cggcttttac
    50701 tacccgatga ctcagccgat taaagatgcg gtggaaacgg tgtatcagcg gctggaaggc
    50761 tgggaaggaa atggcggctt cgcgcagtta gcggtggaag aataattctt cattagggaa
    50821 ttcagggaat tgaacatcaa aacagggatg tgcgcttttt tattgagctt agtgctgcct
    50881 gcacaggcca ccagctttac cgaatatctg ccaatgagtg acagcgaata cgcgcaaaag
    50941 cgggcgctga aacctctcct gaccatgcct tatgatgccg agcaaacctg gcattttaga
    51001 aaagtgggcg tctgacacgt tactacagag agaacaaaga atgactgcta atgctgcacg
    51061 cgctgttaaa gcaacaagag aactggtcaa tgcagtcccc tttctgggag gcagtgactc
    51121 agaagatgat taccgtgaag cactggaact ggtcgaatat ttgattgaag aagatgatac
    51181 taatccactt atcgactttc tggcgagccg cattgcagaa tatgaaaaca ataacgaaaa
    51241 gtttgcagag ttcgacaaag ccgttgcagc aatgcctgtt ggtgtggcgt tacttcgcac
    51301 gctgattgat caacataatc tgacctatgc agatttgaag aacgaaatcg gctctaaatc
    51361 cctggtcagc cagattttat ctggacaacg ttccctgacg atatcccaca ttaaggcact
    51421 ttcagcccga tttggcgtta agccggaatg gtttctttga ctgatggcag acgctaaatc
    51481 gcctgatgcg ctacgcctat cgggctttga aagggcgcag attcttaatt ttgcgcgccc
    51541 ttgtttgtca ttcaggctgt tgtctttact actcttaatt tatcagtaag ctcaatcaga
    51601 tatgcttttg ctttttcgcg tgctcgcccc tcgaaaagca ttatccactc atgtgtgtcg
    51661 tcgattgttt gtaaacggcc ctggagtttt ttgctgaaag tcgacgtgag ctgataaccg
    51721 tcgagttgat caatttgttt actcgatgtg atttttttcc cggctgcttt ttgaatgata
    51781 agttcaacga gaccgataac attattatcg taattatcgt agagaaattc ttcatctccc
    51841 tgaaatccga acaaatcttc atgctggctt aagcgttgaa caattgactc ggcatcaaag
    51901 gtcgaatcat cttgcagttg ttcctctacc caattaatca aatccagccc ttcgtcgctt
    51961 tcgagagcct ccattcccca tgaacccatt ttgtatcctt acctgttatt tcagtcgaaa
    52021 tagtattatt caataaaggt tcatcgctaa tctgcgatga acctcaaaat acaatcatat
    52081 ggatagctga caaagtggtc gttgttcacg ccgtatccgg cacggttgct tcccttaaat
    52141 cctccccgtt actggcaatt acttttttat accaccagaa cgacttgtcg gaaaaaagta
    52201 cccagatgtg gactggcaac gataaaatat tcattagaaa acggattctc tctgccttac
    52261 tacgataaat actgtcatca catgtcatcg acactagtgt cgatgacatc gtttttcctg
    52321 tagtacagaa tttaatttcc ttaaataaca gtaaattaaa aactggcatg atttgtgaat
    52381 gtatcggcgc attaactgtt attgctggag aatttgatga accgtttcat cattgctgac
    52441 gcgagtaaat gtattggttg ccgtacctgt gaagtggcgt gcgtggtttc tcatcaggaa
    52501 aatcaggact gtgcatcgct gaccccggaa acatttttac cgcgaatcca tgtcattaaa
    52561 ggtgtgaaca tttccacggc gacagtctgc cgtcagtgtg aagatgcacc gtgcgctaac
    52621 gtctgcccga atggtgctat cagccgtgat aaagggtttg ttcatgtcat gcaggaacgt
    52681 tgtattggtt gcaaaacctg cgttgtggct tgcccatatg gtgcgatgga agtggtggtg
    52741 cgtccggtga ttcgcaacag cggcgcaggg ctgaatgtac gggctgacaa agccgaagcc
    52801 aataaatgcg acctgtgcaa ccatcgtgaa gacggcccgg cgtgtatggc ggcttgcccg
    52861 acccatgcgc tgatttgtgt cgatcgtaat aaacttgaac aactgagcgc agaaaaacgc
    52921 cgccgcacgg cgctgatgtt ctaaaaatct tacagaactg ttctggtatc aaaggaattc
    52981 ccggaaatat tcgtagaata cgaagcgggc agtatcgccg acctgtgact gccggatgcg
    53041 acgctgtcgt gtccggctgg gcgggttacg caattcatga tggcgggaat aactcaatgg
    53101 caaaaaacac atcttgcggt gtccaactgc gtattcgtgg caaagtgcag ggcgtcggtt
    53161 ttcgtccgtt tgtctggcaa ctggcacagc aattaaatct tcacggcgat gtctgtaatg
    53221 acggcgatgg cgtagaagtc cggctgctgg aagagccgga aacctttctt gttcaattgc
    53281 atcagcattg tccgccgctg gcgcgtattg atagcgtcga gcgtgagccg tttatctggt
    53341 cacaactgcc caccgagttc actatccgcc agagcacagg cggcgctatg aatacgcaaa
    53401 tagttcccga tgccgcgact tgccctgctt gccttgccga aatgaatacc ccgggcgagc
    53461 ggcgttatcg ttatccgttt atcaactgta cccactgtgg cccgcgtttc accattattc
    53521 gcgccatgcc ttacgaccgc ccgtttaccg tgatggcggc gtttccgctg tgtccggctt
    53581 gtgacaaaga gtactgtgac ccgctcgatc gtcgcttcca cgcccagccg gtggcctgcc
    53641 cggagtgtgg cccccatctt gaatgggtaa gtcatggtga acatgcagaa caagaggcgg
    53701 cattacaggc agctatcgca cagttaaaaa tgggcaacat tgtcgccatc aaagggattg
    53761 gcggatttca tcttgcctgc gatgcacgta acagtaacgc ggtggcgaca cttcgggcgc
    53821 gcaaacatcg cccggcgaaa ccgctggcgg tcatgttgcc agtggctgac ggtttaccag
    53881 acgctgcgcg ccagttgctt accacgcccg ccgcgccgat tgtgctggtg gataaaaaat
    53941 atgtacctga gctttgtgat gatatcgccc ctggccttaa cgaagtcggg gtaatgttgc
    54001 ctgcgaaccc gctccagcat ttgctgttac aggaactgca atgcccgctg gtgatgacct
    54061 ccggcaacct gagcggtaaa ccaccggcta tcagcaacga acaggcgctg gcggatttgc
    54121 agggcattgc cgacggattc ttgatacata accgcgacat cgtgcagcgg atggatgatt
    54181 cggtggtgcg cgaaagcggc gaaatgctgc gccgttcgcg ggggtatgtg ccggatgcgc
    54241 tggctttgcc tccgggcttt aaaaatgttc cgcctgtgct gtgtctcggc gcggatctga
    54301 aaaatacctt ctgcctggtg cgcggcgaac aagcggtgtt gagtcagcat ctgggcgatt
    54361 taagtgacga tggcatccag atgcagtggc gcgaagcgtt acgcctgatg caaaacatct
    54421 acgatttcac tccgcaatac gttgtgcatg acgcgcatcc gggctatgtc tccagccagt
    54481 gggcgcgcga aatgaatctg ccgacgcaaa cggtgctgca tcatcatgct cacgcagcgg
    54541 catgtctggc agaacatcac tggccgctgg atggcggaga tgtcattgcg ttgacactcg
    54601 acggtattgg tatgggggag aacggcgctt tgtggggggg agagtgcctg cgggtgaact
    54661 atcgtgaatg cgagcacctg ggcggcttgc ctgcagtggc gcttgctggt ggcgatcttg
    54721 ccgcaaagca gccgtggcga aacctgctga cgcagtgctt gcgttttgtg ccggagtggc
    54781 agagttatcc cgaaacggta agtgtgcaac agcaaaactg gagcgtgctg gcgcgggcca
    54841 ttgagcgtgg aatcaatgca ccactggcgt catcgtgtgg gcggttgttc gatgccgtag
    54901 cggcggcgct gggctgtgcg ccagccacgt taagttatga aggtgaagcg gcttgtgctc
    54961 tggaggcgct ggcgacttcg tgccacggcg tgacgcatcc ggtgacgatg ccgctggtgg
    55021 ataatcaact ggatctcgcc accttctggc agcagtggct gaactggcag gctccggtta
    55081 atcaacgtgc gtgggcgttt catgatgcgc tggcgcaggg gggggccgcg ttgatgcgtg
    55141 agcaggccac gatgcgtggt atcactacgc tggtatttag cggcggggtt attcataacc
    55201 gtttgctgcg tgcacgtctg gcgcattatc tcgctgattt cacattgctc tttccacaga
    55261 gtttacctgc gggcgatggc gggttgtctc tggggcaggg ggttattgct gcggcgcgtt
    55321 ggttagcggg tgaagtccag aacggataaa gcgtgttgca tttggacacc attgccggat
    55381 gcggcgtgaa cgccttatcc ggcctacata ttcatccccg caggtcggat aagacgcgct
    55441 agcgtcgcat ccgacatttt gggacagtgc aatgcgacat aaacgtatta tgtgatcaac
    55501 acatgcgttc cctgtaggtc ggataagacg cgtcagcgcc gcatccgaca tttggggcac
    55561 tgtagctcgc ctacatcgac aacgttttca acaatccaaa tgcctctttc atccgatcct
    55621 cactgaccac aaaggcgcga agctggtcag catcgtcaac accgcgcgcc accattccct
    55681 ggcgttcggt attaatttgc cagcgtaaat cctgacgctg gggttcgcct gccagatgca
    55741 gcggtaattc cggcgttttg attttcacca gcatcgccgg gagtttcagc ggcgtgttat
    55801 tgccgagaag attttttgcc agcaccatcg cgctaagttg aatcggctgg aggaacggca
    55861 atacctgacc gttaatttcc gcgcaatcgc ccagcgcata aatatcggca ttactggttt
    55921 gcagataact atcgacgcaa acgccgcgat taatcgtcag cccggcgcgt cgtgccaggg
    55981 cggtttccgg gcgcagtccg gtggcggcaa ttaccgcatc cacttcgatg cagcgctggc
    56041 ggtccagcgt tgccagaatg ccagaatctg ttttttccag tccttgcaac tgagatttta
    56101 acagcagatg aacgcccatc tccgtcaacc gatgctgcaa gcggctgctg acttccggtg
    56161 gcattaacga cgccagaata ctggcagcgt tgtcgattag cgtgaccgct ttgcctgccc
    56221 gacaaaaatc catcgccagt tcgctaccaa tcaaaccacc gccaacaatc aacacgcgtc
    56281 gggcatcccg cagttgtgtt tcacaggcgc gatactcttg ctgactattt aacgtcagca
    56341 ttaactcacg cccgggcact ggcgggacaa aggcgctggc accggttgcc agcactaact
    56401 tgtcgtattg ccactgatta ttctgacttt tcaccacatg ggcttcggca tcgatatccg
    56461 ttacccaggt gtgcggaaac aggcgcagat taaactgctc ggcaaattca cctgccgtct
    56521 ggcgggtaag gtcgtcggca cgttgcccct gactgataac atggctgagg tcaggtttgt
    56581 tgtactcatc catgctgtcg gcggcaatca gggttaatgg aatacaggcg tcctgtttgc
    56641 gaatattttt caccagttgg cgggcggcga agcccgaacc aatgatcaca atgccgttgc
    56701 tcattttgcc tccgatgcca gttcgtcaaa gacatctttg ccgagagagc attccgggca
    56761 caggaagtta tccgggactt cactccacgg cgttcctggc gcaacgtcct gcattggctc
    56821 gccttttgcc ggatcgtaaa tccactggca gacgctgcat tgcatccgtg ggccgaggtc
    56881 agccgtcgtg gcggcagagg tttcttcttt aaccaccgta ttcaccgtgc tctgtggcag
    56941 cggcgcgagc gcccactgac gggcgatttc gcgaccgtgt tcacgacata actccagagc
    57001 gtcctggtct ggtcgccatt tggctttcag gctaagcgac atttcgaaac ccgcatcctg
    57061 caggcgcgtg gaaagacgat ccaccgcacc gccactccag ccgtgagagc cgaaagcgct
    57121 ggcgcgtttg ttacggaagc gtaatccggt catctcctcc accagcccgg cgattttcgg
    57181 catcatcaca ttattcatcg tagaagtgcc gaccagtacg ccttttgagc ggaagacatt
    57241 ggtcaggatt tcgtttttat cgcttcgggc gacgttgaaa attttcaccg ccacgcgtgg
    57301 gtcggtttcc gcaatcccct gggcgatagc gtcagccatc atgcgggtgt tattcgacat
    57361 ggtgtcgtag aaaatggtga ttctgtcttc ctgataatcc gccgcccatt tcaggtacag
    57421 ctcgacaatt tgcgtcgggt tatcgcgcca taccacgccg tgagaggtcg ctatcatatc
    57481 gactggcaag ttaaagccca ggatctcggt aattttcggc gttaccaggc ggctgaacgg
    57541 cgtcaggata ttggcgtagt aacgctggca ctgctcgaaa agctcagtct gatccacttc
    57601 atcgttgaac agatgctcgt cgcagtagtg ctgaccgaaa gcatcgttac tgaacagcac
    57661 cgcgtcgcct gtcaggtaag tcatcatgct gtccggccag tgcagcattg gcgtttcgac
    57721 aaaaatgagt tgtttgccgt tgccgatatc cagtgtgtcg ccagttttca ctacattaaa
    57781 attccactcc ggatgatggt gatgaccatt tatcgagtcg atagcgttgg ctgtacagta
    57841 gatcggcgta tcgggaattt gcgccatcag ttcggtcagc gctccggcgt ggtcttcttc
    57901 tgcatgatta atcacgatgt aatcgatatc cgccagatcg atttcattac gcaggttctg
    57961 cacaaattcg cggctgaatt tatggtcgac ggtgtcgatc agcacgtttt tttcttcgcg
    58021 gatgaggtag ctgttgtagc tgctgccgcg cagcgtttta tattctgtac cgtgaaaatc
    58081 acgcacttcc cagtcacgtt gaccaaccca atgaatgtta tttttcacca caatagacat
    58141 agcaacctca atttattcag cgtgttctaa aaagatgtct tgctgattgc agattgcgtg
    58201 ccagtttttt atctaattgt ttttactcta taaataaaat tatgctcatt aatgagtagt
    58261 caaaatgact atcaatcaaa tggtcgatat gacaatatct atagtcaaat tgacagtgag
    58321 gcaaagatga gtttttccgt tgatgtgctg gcgaatatcg ccatcgaatt gcagcgtggg
    58381 attggtcatc aggatcgttt tcagcgcctg atcactacgc tgcgtcaggt gctggagtgc
    58441 gatgcgtctg cgttgctacg ttacgactcg cggcagttta ttccgcttgc catcgacggg
    58501 ctggcgaagg atgtactcgg tagacgcttt gcgctggaag ggcatccacg gctggaagcg
    58561 attgcccgcg cgggtgatgt ggtgcgcttt cccgcagaca gcgaattgcc cgatccttat
    58621 gacggtctga ttccagggca ggagagtctg aaggttcacg cctgcgttgg tttgccgttg
    58681 tttgccgggc aaaacctgat cggtgccttg acgctcgacg ggatgcagcc cgatcagttc
    58741 gatgttttca gcgacgaaga gttacgcctg attgctgcgc tggcggcggg agcgttaagc
    58801 aatgcgttac tgattgaaca actggaaagc cagaatatgc tgccaggcga tgccgcgccg
    58861 tttgaagcgg tgaaacagac gcagatgatt ggtttgtccc caggcatgac gcaattgaaa
    58921 aaagagattg agattgtggc ggcatccgat ctcaacgttt taattagcgg tgagacggga
    58981 accggtaagg agctggtggc gaaagcgatt catgaagcct cgccacgggc ggtgaatccg
    59041 ctggtctatc tcaactgtgc cgcactgccg gaaagtgtgg cggaaagtga gttgttcggg
    59101 catgtgaaag gggcgtttac tggtgctatc agtaaccgca gcgggaagtt tgaaatggcg
    59161 gataacggca cgctgtttct cgatgagatc ggcgagttgt cgttggcatt gcaggccaag
    59221 ctgctgaggg tgttgcagta tggcgatatt caacgcgttg gcgatgaccg cagtttgcgg
    59281 gtcgatgtgc gcgtgctggc ggcgactaac cgtgacttac gcgaagaggt actggcaggg
    59341 cgatttcgcg ctgacttgtt ccaccgcctg agcgtgtttc cactttcagt gccgccgctg
    59401 cgtgagcggg gcgatgatgt cattctgctg gcggggtatt tctgcgagca gtgtcgtttg
    59461 cggctggggc tctcccgcgt ggtattaagt gccggagcgc gaaatttact gcaacactac
    59521 aattttccgg gaaacgtgcg cgaactggaa catgctattc atcgggcggt agtattgtcg
    59581 agagccaccc gcagcggcga tgaagtgatt cttgaggcgc aacattttgc ttttcctgag
    59641 gtgacgttgc cgccgccaga agcggcggcg gtgcccgttg ttaagcaaaa tctgcgtgaa
    59701 gcgacagaag cgttccagcg tgaaaccatt cgtcaggcac tggcgcaaaa tcatcacaac
    59761 tgggccgcct gtgcgcggat gctggaaacc gacgtcgcca acctgcatcg gctggcgaaa
    59821 cgtctggggc tgaaggatta aataatcccg gcctgataga aatcctgcag gttaattgcg
    59881 ccggtgagtt tgccgttttc atccactacc ggtgcggcgg tgatttttcg tttcatcagg
    59941 atttctttgg cgtcgatggc acggctttgc gattgcaacg tggtgccgtc gaccgtcatc
    60001 gcttcattga ctggcgtggt gagtgcgccg ccgccaacca gccagcgacg taaatcaccg
    60061 tcggtaaaga cgcctttcac cagtcgttga tcatcgcaca ctgccaccag acctaaaccg
    60121 gtgcggctaa gttccagcat cgcgtccatc acgctggcgg ttaacgctac ctgcgggatg
    60181 gcatcgtcac gacgcataag atgatgcact ttgttcagca agcgagcgcc cagtgccccg
    60241 gctgggtggg agcgtgcaaa atcttcttca ttaaaaccac gcgcctgcat gactgccatc
    60301 gccagcgcgt cgcccatcat cagggtattc acggtgctgg aggtcggcgc aaggtgcatc
    60361 gggcaggctt cgcgttctac ggagatatcc agcaccgctt tcgccgccag gcccagcggt
    60421 gacgtcggtt ttccggtcat tgccagcagc gcgatagatt tgtcttccag acgcggaata
    60481 atgagatcca gttccttcgc gccaccggaa taagagataa acagcatcac atcgcggctt
    60541 tcaatcatcc ccagatcgcc gtgcagtgct tctgccggat ggacaaaaaa agccggtgtg
    60601 ccggtgctgg caagcgttgc ggcgattttc ttaccaatgt ggcccgattt gccaattccc
    60661 gaaaccacca ctttgccttc gcagtgcagg atgatattgg cggcgcgaac gaaatcatcg
    60721 cccagacgtt ccggtaaatg acttgcttcc tgcaactcca gcattaacgt ctgacgtccc
    60781 gcgttcagta gtgcttcact cattgctctc tccggttatg atcacgtcga tccctttttc
    60841 ttccagcgcc tgacgaaacg ctggatcgat acctgcgtcg gtaatcagct tatcgacgct
    60901 ttcaagacta caaaccacgt tggggctttt acgaccaaac ttcgatgagt cagccatcaa
    60961 aatcacttcg cgcgcggcat tgcacatcgc cttactgacg gtataaacct cgttgaacgt
    61021 ggttacgccc gcattgagat cgatgccgtc ggtgcccata aacaatttat cgaaggtgaa
    61081 atgctcgaag gcattctctg ccagttgccc gtgaaatgag gccgattttt tacgaaatgt
    61141 tccgcctggc atcaggatag tttgttcgtt atccagttcg gatagcgcat tgacgatgtg
    61201 cagactgttg gtcatcaccg tgatgttatt aaaacgtgag agcagaggca ccatctgcaa
    61261 cacggtactg ccagcatcaa gaattattga gtcgccatca tgaataaaac caacggcagc
    61321 ttctgcaatc agctctttct tgtgggtgtt gataagcgtt ttatgatcga taggcggatc
    61381 ggattcctct ttgttcaaca ccacgccgcc ataagtacga atgacggttc cggcatgttc
    61441 cagaatgacc agatctttgc gaatggttgt gcctgtggtg tcaaagtatt gcgccaattc
    61501 ttcaaccgag catttaccct gcttttgcag atactccaga atggcggcct gacgctgacg
    61561 aggtttcatg ggcgtgatta cctgatttag gtttcaaaat gataacttcg caagctagta
    61621 acattcacaa cgaaattatc cacgtttcag tttaagcgcc aatgatagag cattctgtga
    61681 tagtggatca tggggaaaga tcacatcggg ctgtaaatca cccgcatgca tgccggtaat
    61741 tgcgggaatt ttttgcggtc gggcgaagac ggtcagtcct ttcataaaca aggtgtcgac
    61801 gatgcgctgc tgatcgtcca gcgcgatggc gaccacgacc cgtggtttaa agcgcccgct
    61861 ggaacgtccc acgccaacgc gcccttgttg gcatagtgtg tcgaaggcac ggttaaaacg
    61921 agaaatttgc caaccgccta aggccagttg cgcacaccag gcgataacgg cgacggtgat
    61981 gagtgcggat accataactt ctccgtgatg tagtggcgga agtcctgtag gcctgataag
    62041 cgtagcgcat caggcattct attagtcgcg cggagatcct taaaagatct ccgctgttga
    62101 tcagaacatc acctgaccgc cggtgacatt gatcgactga ccagtgcagt acgacgcctt
    62161 aggactggcg tagaacagca gcatattcag cacatcctga taatcgcagc cgcgtttgag
    62221 cggtacttta tcgatgtaat actgctcgac ttgctccggt ttgataccca gcttggtcgc
    62281 gtattgtggc aacagtgact ggaacatagg cgatttcagc aggttaccga gcatcagcga
    62341 atgcaccgta atgccgtact ccgccagatc cagcgccagt gattgagtca gcccgacgcc
    62401 accaaatttc gctgcgctgt agccagagtt gtgtttgctg cccactttgc cggatttcga
    62461 gttgatctga ataatgcgac cctgaatccc gtcgcgaatc atcaaacgcg aaaattcacg
    62521 ggcacacagg aaataaccca ccagattcac ctgtagcgaa cggtcaaaat ctccaagctg
    62581 gaaatcgctg ataaaggctg cttttgctat tccggcgctg tagaccagca aatccacgcg
    62641 accaaagatt tcatctaccc cacgagaaag cgccagaacg ctttgctcgc tggtggcgtc
    62701 agcaccaaaa ccgtacgctg ttccttcacc atattcggcg ttaatttctt gtgccacatt
    62761 tgcggctttg tcgctctgaa tatcgacaac cgccacgcga tacccctcgg cagccagacc
    62821 gtggcacagg aacgcgccta aggtttgccc accaccgatg acaacggcaa cctgattcat
    62881 tttttactcc ttaacagatt caaacttcaa aaccgatccc ggcgcgatat cgtcgggtac
    62941 agggcctgcc acatggacag tgcccggaaa ttccgcttcg cttaaaccat cgaagcgcag
    63001 ggtgacatga cccagttcgc gaaggttgtc ttccgccacg ctgccaacgg cggtcaccgg
    63061 atagcgatgc cgcccgagcg taaattgcaa accgggatgg agtgcacctt tcaactcgcc
    63121 gtggcaatga atgaagcaat actcttcgag atccgctggc gcgccttcac gaaaggtgat
    63181 gagcatctgg tcgctgaggg cgtcagtggc actcgcgcca atacgggtga tggtggtctg
    63241 ataaataacg gtcatgtttc agcctctatt gatagataaa accggagaca aaccaggcga
    63301 tcagtacagt tggcgcgccg gttaaaaagc ggctcaccag aacagaaggg acaccgacgc
    63361 gaaccgtgtc ctgacgagct tccgccagcg acaaaccgac cgggatgaag tcgcaggccg
    63421 cctgcgcgtt gatggcaaac agtgccggta aagccagatg cggcggaata ttgccgagac
    63481 caatctgcac gccaatcaat acgccgataa cctgtgcgat aactgcgcct gggccgagga
    63541 aaggtgaaag cagcgggaag gagcagatga gcgccagcat gaccagaccc agtggatggc
    63601 tcgccagcgg agcaagaccg tgggcaatcc agtcgccaag accggaagcc ataatgatgc
    63661 cgataagcgc cgagacgaac gccataaacg gcagaatggt ttttaataca gtgtcgatgg
    63721 tgtcacgacc agattgaaac aacacggcaa cggcagaacc catacccatt cccaccttcg
    63781 ccagtaaacc atcgctttgt tcggtgattt tcttgctggt gtcatagtca cggcccacgg
    63841 aagagggttg tggtgtcgcc tcacccacta cagtgatgtt ttcttctttt acgccagaga
    63901 cataaatatc ttccacaatg tactgcgcca gcgggccaga cttgcccgtc gagtggatat
    63961 taatggtggg aatacgccgt ttcggataga tgccgcagcg taatgtgccg ccacagtcga
    64021 ttaccgcgac accaatttcc gcctccgcgg gttcaccttc tttaaatccg tcaatagcct
    64081 gccagccagt aagctgtgcc agtttgtcaa caatcgcagg ccgggtaccg gcggtgatat
    64141 agacgatttt tttgcccggc gtggcttcca gctcaagcgg gccgccccag ccacccgttc
    64201 ctttttcgat ccgaatgcgt gtcatgatgt tgctcctgcc agacgaactt tttgttcaag
    64261 ttgaatgccc atctttttct cgaaaatggc ggtggtcaga tcggttaccc agccgcggaa
    64321 gaagttggtg accagaccaa ccagcagata gctcaccgcc agtgggccaa gtggcagatt
    64381 cagcgtcgtc agaccgctgg caatgccgag ataaacaaac agttcgccag ggttgatatg
    64441 ggggaagagg ccattcattg agtggcagct ataagaggcc gccgcgtaat agctgggttt
    64501 gtacttttcc ggcataaagc gacccaggct tagggtcatc gggttacaaa agacaaacgt
    64561 accaatgcac ggtagcagca gataacggga aacagggtta ccggcgcaac gttgagcaaa
    64621 tcgttcaata cgatgctgac cgataaaatt aatcaatgcg ttcatgataa ccagcaggct
    64681 aattaacagc ggaagaatgc cggttaccat cccggtaaac acttctccgc ctttctggaa
    64741 cagcccgatg aaccactctg cgccatgagt aatggtttct atcattgttc tctccttcag
    64801 tatttattgt tttattacca aacggcaatc taatctaatc agattgaaag atttaaaagt
    64861 gttattttga tcgcaaaatg aaagataaat attttatttt gaaagtttgt ataaagaagg
    64921 tagtgaggag aatgagttga gttatgtaaa gtccgtatcg ggcagtgact accgcttcct
    64981 tgtgcggggc gggatgcttt accatacttg cccctggttg aatctgttaa atggacccct
    65041 catgttcaag cgtcgttatg taacattgct tccccttttt gtgttgcttg ccgcctgtag
    65101 cagcaagcca aaacctactg agactgaaac gaccaccgga acgccgtctg gcggcttcct
    65161 gcttgagccg cagcacaatg tgatgcagat gggcggtgac ttcgccaata acccgaatgc
    65221 ccagcagttc atcgacaaaa tggtgaacaa acacggtttc gatcgtcagc agttgcagga
    65281 aattctctcc caggcgaaac gtctggattc ggtactgcgg ctgatggata accaggcacc
    65341 aaccacatcg gtgaaaccgc catcaggtcc gaacggcgca tggctgcgtt atcgcaaaaa
    65401 atttattacg ccggacaacg tgcagaacgg tgtggttttc tggaatcagt atgaagatgc
    65461 gttgaatcgc gcgtggcagg tgtatggtgt accgccggaa attatcgtcg ggattatcgg
    65521 cgttgaaacc cgctgggggc gcgtgatggg gaaaactcgc attctcgatg cgctggcaac
    65581 gctgtcattt aattaccctc gccgcgcgga atatttttca ggtgagctgg aaaccttcct
    65641 gctgatggcg cgcgacgagc aggacgatcc gctcaatctg aaaggttcct ttgcaggtgc
    65701 gatgggttac ggtcagttta tgccatcgtc ttacaaacaa tatgcggtgg atttcagcgg
    65761 tgacgggcat atcaacctgt gggatccggt tgatgccatc ggtagtgtag cgaactattt
    65821 caaagcgcac ggctgggtga aaggcgatca ggtggcggtg atggcaaacg gtcaggcgcc
    65881 aggcttaccg aatggcttca aaaccaggta cagcatttca caacttgccg cagctggact
    65941 aacgccgcag cagtcgctgg ggaatcatca gcaggccagc ttgctgcgtc tggatgttgg
    66001 cactggttac cagtactggt atggtctgcc gaacttctac actattaccc gttataacca
    66061 cagcactcat tacgcaatgg cggtctggca gttaggacag gccgtggcgc tggcgcgcgt
    66121 gcagtaacta tttgcaagag ggaggttaaa cttccctctg ttatcttact gtttattcga
    66181 atggcgtaat tgcttgattt ttttcgacag cgatatcgca tatcccaggc agacaacgcc
    66241 taacgcagcc tgaccgaagt caaaaggttc aagatagcca gaagataaaa ctttacatat
    66301 cacggaaatc agtgcgctga tgccgccaat gatgacccac atacgcattt gtgtggtttg
    66361 tttgagcacg ttttgtgggg tgttatccgt tttctttatt gccattgcta ttctctccct
    66421 gtgaatacat atcaatgtga acgcggcatg gtagcgcttt ttctctaccg actggccgca
    66481 acagtctttt ttcggaacgc gactcgtaaa atgaatcgct cgtccccatc ttttgcggga
    66541 aagtgctatc ttgtccggca taaatttttg actgacagag gttgtgatga ctgaaagtga
    66601 actgatgcag ttaagcgaac aggttgggct ggcgctgaaa gcccgtggcg caaccgtaac
    66661 aactgctgag tcttgtaccg gcggttgggt agcgaaagtg attaccgata ttgccggcag
    66721 ttccgcctgg tttgaacgcg gatttgtcac ctacagtaac gaagcaaaag cgcaaatgat
    66781 cggtgtacgc gaagagacgc tggcgcagca tggcgcggtg agcgaacctg tcgtggtgga
    66841 aatggcgata ggcgcactga aagcggctcg tgctgattac gccgtgtcta ttagtggtat
    66901 cgccgggccg gatggcggca gtgaagagaa gcctgtcggc accgtctggt ttgcttttgc
    66961 cactgcccgt ggtgaaggca ttacccggcg ggaatgcttc agcggtgacc gtgatgcggt
    67021 gcgtcgtcag gctactgcgt atgcattgca gaccttgtgg caacaatttc tacaaaatac
    67081 ttgatactgt atgagcatac agtataattg cttcaacaga acatattgac tatccggtat
    67141 tacccggcat gacaggagta aaaatggcta tcgacgaaaa caaacagaaa gcgttggcgg
    67201 cagcactggg ccagattgag aaacaatttg gtaaaggctc catcatgcgc ctgggtgaag
    67261 accgttccat ggatgtggaa accatctcta ccggttcgct ttcactggat atcgcgcttg
    67321 gggcaggtgg tctgccgatg ggccgtatcg tcgaaatcta cggaccagaa tcttccggta
    67381 aaaccacgct gacgttgcag gtgatcgccg cagcgcagcg cgaaggtaaa acctgtgcgt
    67441 ttatcgatgc tgaacacgcg ctggacccaa tctacgcacg taaactgggc gtcgatatcg
    67501 acaacctgct gtgctcccag ccggacactg gcgagcaggc actggaaatc tgtgacgccc
    67561 tggcacgttc tggtgcagta gacgttatcg tcgttgactc cgtggcggca ctgacgccga
    67621 aagcggaaat cgaaggtgaa atcggcgact ctcacatggg ccttgcggca cgtatgatga
    67681 gccaggcgat gcgtaagctg gcgggtaacc tgaagcagtc caacacgctg ctgatcttca
    67741 tcaaccagat ccgtatgaaa attggtgtga tgttcggtaa cccggaaacc actaccggtg
    67801 gtaacgcgct gaaattctac gcctctgttc gtctcgacat ccgtcgtatc ggcgcggtga
    67861 aagagggcga aaacgtggtg ggtagcgaaa cccgcgtgaa agtggtgaag aacaaaatcg
    67921 ctgcgccgtt taaacaggct gaattccaga tcctctacgg cgaaggtatc aacttctacg
    67981 gcgagctggt tgacctgggc gtgaaagaga agcttatcga gaaagcaggc gcgtggtaca
    68041 gctacaaagg tgagaagatc ggtcagggta aagcgaatgc gactgcctgg ctgaaagata
    68101 acccagaaac cgcgaaagag atcgagaaga aagtacgtga gttgctgctg agcaacccga
    68161 actcaacgcc ggatttctct gtagatgaca gcgaaggcgt agcagaaact aacgaagatt
    68221 tttaatcgtc ttgtttgata cacaagggtc gcatctgcgg cccttttgct tttttaagtt
    68281 gtaaggatat gccatgacag aatcaacatc ccgtcgcccg gcatatgctc gcctgttgga
    68341 tcgtgcggta cgcattctgg cggtgcgcga tcacagtgag caagaactgc gacgtaaact
    68401 cgcggcaccg attatgggca aaaatggccc ggaagagatt gatgccacgg cagaagatta
    68461 cgagcgcgtt attgcctggt gccatgaaca tggctatctt gatgacagcc gatttgttgc
    68521 gcgctttatc gccagccgta gccgcaaagg ttatggacct gcgcgtattt gtcaggaact
    68581 gaatcagaaa ggtatttccc gcgaagcgac agaaaaagcg atgcgtgaat gtgacatcga
    68641 ctggtgcgca ctggcgcgcg atcaggcgac gcgaaaatat ggcgaacctt tgccaactgt
    68701 cttttcagaa aaagttaaga tccagcgttt tctgctctat cgtggctatc tgatggaaga
    68761 tatccaggat atttggcgaa attttgccga ctgaacgcat acgggatttt acttcccagt
    68821 caagaaaact tatcttattc ccacttttca gttaccagcc gggcggttaa cgacacgcaa
    68881 cggctggtag cgatatttcg ttagcttgat ttcaggataa ttatgagcaa gagcaccgct
    68941 gagatccgtc aggcgtttct cgactttttc catagtaagg gacatcaggt agttgccagc
    69001 agctccctgg taccccataa cgacccaact ctgttgttta ccaacgccgg gatgaaccag
    69061 ttcaaggatg tgttccttgg gctcgacaag cgtaattatt cccgcgctac cacttcccaa
    69121 cgctgcgtgc gtgcgggtgg taaacacaac gatctggaaa acgtcggtta caccgcgcgt
    69181 caccatacct tcttcgaaat gctgggcaac ttcagcttcg gcgactattt caaacacgat
    69241 gccattcagt ttgcatggga actgctgacc agcgaaaaat ggtttgccct gccgaaagag
    69301 cgtctgtggg ttaccgtcta tgaaagcgac gacgaagcct acgaaatctg ggaaaaagaa
    69361 gtagggatcc cgcgcgaacg tattattcgc atcggcgata acaaaggtgc gccatacgca
    69421 tctgacaact tctggcagat gggtgacact ggtccgtgcg gcccgtgcac cgaaatcttc
    69481 tacgatcacg gcgaccacat ttggggcggc cctccgggaa gtccggaaga agacggcgac
    69541 cgctacattg agatctggaa catcgtcttc atgcagttca accgccaggc cgatggcacg
    69601 atggaaccgc tgccgaaacc gtctgtagat accggtatgg gtctggaacg tattgctgcg
    69661 gtgctgcaac acgttaactc taactatgac atcgacctgt tccgcacgtt gatccaggcg
    69721 gtagcgaaag tcactggcgc gaccgatctg agcaataaat cgctgcgcgt aatcgctgac
    69781 cacattcgtt cttgtgcgtt cctgatcgcg gatggcgtaa tgccgtccaa tgaaaaccgt
    69841 ggttatgtac tgcgtcgtat cattcgtcgc gcagtgcgtc acggtaatat gctcggcgcg
    69901 aaagaaacct tcttctacaa actggttggt ccgctaatcg acgttatggg ctctgcgggt
    69961 gaagacctga aacgccagca ggcgcaggtt gagcaggtgc tgaagactga agaagagcag
    70021 tttgcccgta ctctggagcg cggtctggcg ctgctggatg aagagctggc aaaactttct
    70081 ggtgatacgc tggatggtga aactgctttc cgtctgtacg acacctacgg cttcccggtt
    70141 gacctgacgg ctgacgtttg ccgtgagcgc aacatcaaag ttgacgaagc tggttttgac
    70201 gcagcaatgg aagagcagcg tcgtcgtgcg cgcgaagcca gcggctttgg tgcagattac
    70261 aacgcaatga tccgtgttga cagtgcatct gaatttaaag gctatgacca tctggaactg
    70321 aacggcaaag tgactgcgct gtttgttgat ggtaaatcgg ttgatgccat caatgcaggc
    70381 caggaagctg tggtcgtgct ggatcaaacg ccattctatg cggaatccgg cggtcaggtt
    70441 ggtgataaag gcgaactgaa aggcgctaac ttctccttcg cggtggaaga tacgcagaaa
    70501 tacggccagg cgattggtca catcggtaaa cttgctgtgg gttctctgaa agtgggcgac
    70561 gctgtgcagg ctgatgttga tgaggctcgt cgcgcccgta ttcgtctgaa tcactccgca
    70621 acgcacctga tgcacgctgc gctgcgccag gttctgggta ctcatgtatc gcagaaaggt
    70681 tcactggtta acgacaaagt gctgcgcttc gacttctcac acaacgaagc gatgaaacca
    70741 gaagagattc gtgcggtcga agacctggtg aacgcgcaga ttcgccgtaa cttgccgatc
    70801 gaaaccaaca tcatggatct cgaagcggcg aaagcgaaag gtgcgatggc gctgtttggc
    70861 gagaagtatg atgagcgcgt acgcgtgctg agcatgggtg atttctccac cgagttgtgt
    70921 ggcggtactc acgccagccg cactggtgat attggtctgt tccgcatcat ctctgaatcg
    70981 ggtactgctg caggcgttcg tcgtatcgaa gcggtaaccg gagaaggcgc tatcgccacc
    71041 gttcatgcag acagcgatcg cttaagcgaa gtcgcgcatc tgctgaaagg cgatagcaat
    71101 aatctggctg ataaagtgcg ttcagtactg gaacgtacgc gtcagctgga aaaagagtta
    71161 caacagctta aagaacaagc tgccgcacag gagagcgcaa atctttccag taaggcaatt
    71221 gatgttaatg gtgttaagct gttggttagc gagcttagcg gtgttgagcc gaaaatgttg
    71281 cgtaccatgg ttgacgattt aaaaaatcag ctggggtcga caattatcgt gctggcaacg
    71341 gtagccgaag gtaaggtttc tctgattgca ggcgtatcta aggacgtcac agatcgtgtg
    71401 aaagcagggg aactgattgg tatggtcgct cagcaggtgg gcggcaaggg tggtggacgt
    71461 cctgacatgg cgcaagccgg tggtacggat gctgcggcct tacctgcagc gttagccagt
    71521 gtgaaaggct gggtcagcgc gaaattgcaa taatataagc gtcaggcaat gccgtggact
    71581 cgcttcacgg cattcgcatt aacgctatcg acaacgataa agtcaggttg aagttgtgta
    71641 tatcggctaa acttaggttt aacagaatgt aatgccatga ctgcttagat gtaatgtgtt
    71701 tgtcattgct tactttttgg cgttatatga tggataatgc cgggatacag agagacccga
    71761 ctcttttaat ctttcaagga gcaaagaatg ctgattctga ctcgtcgagt tggtgagacc
    71821 ctcatgattg gggatgaggt caccgtgaca gttttagggg taaagggcaa ccaggtacgt
    71881 attggcgtaa atgccccgaa ggaagtttct gttcaccgtg aagagatcta ccagcgtatc
    71941 caggctgaaa aatcccagca gtccagttac taatctttcc gcgtctcatc tttatcggtg
    72001 agacgcaccc tcaagatttc tctttcactc cattgtcttt tcgctttact cccgttcatt
    72061 caacttaagt ctccattttt ttgcattact gctatctgtc agacctccgt ttttctgttg
    72121 ataaaacact ctttttgacg tttttacaga ctaattgagc gtgaagtgtg caaacgataa
    72181 aagtgtggga aaaattgttt gacttataag tctcagaaag taatatgtgc gccacgcagc
    72241 gacgatgagc gataaacaag ttcttcgaag cactcgtaag aggcgtgtgg tgaggtggcc
    72301 gagaggctga aggcgctccc ctgctaaggg agtatgcggt caaaagctgc atccggggtt
    72361 cgaatccccg cctcaccgcc atttgcatcc gtagctcagc tggatagagt actcggctac
    72421 gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atctcttact tgatatggct
    72481 ttagtggcgg tatcaatatc agcagtaaaa taaatttccc gatgcatccg tagctcagct
    72541 ggatagagta ctcggctacg aaccgagcgg tcggaggttc gaatcctccc ggatgcacca
    72601 tctcttactt gatatggctt tagtagcgat atcaatatca gcagtaaaat aaattttccc
    72661 gatgcatccg tagctcagct ggatagagta ctcggctacg aaccgagcgg tcggaggttc
    72721 gaatcctccc ggatgcacca tattctccgt aaccttcagc aatgaaggta tcgaagaggt
    72781 agcggtatta accgcaggca ccagggagga taacgttgcg ccagcagcgg cccgaagggc
    72841 gaacgccagt gagtcatcct cccggatgca ccatattatc caaaaaattt ccttcaaaaa
    72901 tatctcattt ctaaataact actttatcca tttcctgaat tttcttctcc ttctccatca
    72961 gcgacaaaat caagcaatta cgctaaagta gcgtcatatt tttcgacttg cgagaagacc
    73021 atgtacgagc gttatgcagg tttaattttt gatatggatg gcaccatcct ggataccgag
    73081 cctacgcacc gtaaagcgtg gcgcgaagta ttagggcact acggtcttca gtacgatgtt
    73141 caggcgatga ttgcgcttaa tggctcgccc acctggcgta ttgctcaggc gattattgag
    73201 ctgaatcagg ccgatctcga cccgcatgcg ttagcgcgtg aaaaaacaga agcagtaaga
    73261 agtatgctgc tggatagtgt cgagccgctt cctcttgttg aagtggtgaa aagctggcat
    73321 ggtcgtcgcc caatggctgt aggaacgggg agtgaaagcg ccatcgctga ggcattgctg
    73381 gcgcacctgg gattacgccg ttattttgac gccgtcgtcg ctgccgatca cgtcaaacac
    73441 cataaacccg cgccagacac atttttgttg tgcgcgcagc gtatgggcgt gcaaccgacg
    73501 cagtgtgtgg tctttgaaga tgccgatttc ggtattcagg cggcccgtgc agcaggcatg
    73561 gacgccgtgg atgttcgctt gctgtgagtg aagcgttatc gcttttctcg ttgtttgcca
    73621 gtagttttct cagcgctaca ctattacccg gcaactccga agtcgtgctg gtggcaatgt
    73681 tgctttccgg agtgagtcat ccctgggttt tagttttaac agcaacaatg ggtaatagcc
    73741 ttggagggtt aactaacgtt atccttgggc gtttctttcc attgcgtaaa acatcgcgct
    73801 ggcaagagaa agctaccggc tggctgaaac gctatggtgc agtcacgcta ttattaagct
    73861 ggatgcccgt ggttggcgat ttactgtgtc tgttagcggg atggatgcgc atctcgtggg
    73921 gaccggtaat cttttttttg tgccttggta aagcgttacg ctatgttgca gttgcagcag
    73981 ctaccgttca gggcatgatg tggtggcact aattgtaggc ctgcacatat ggtcaccatt
    74041 acagttatgc taattaaaac gattttgaca ggcgggaggt caatttgatc ccggacgtat
    74101 cacaggcgct ggcctggctg gaaaaacatc ctcaggcgtt aaaggggata cagcgtgggc
    74161 tggagcgcga aactttgcgt gttaatgctg atggcacact ggcaacaaca ggtcatcctg
    74221 aagcattagg ttctgcactg acccataaat ggattactac cgattttgcg gaagcattgc
    74281 tggaatttat tacaccagtg gatggtgata ttgaacatat gctgaccttt atgcgcgatc
    74341 tgcatcgcta tacggcgcgc aatatgggcg atgagcggat gtggccgtta agtatgccat
    74401 gctacatcgc agaaggtcag gacatcgaac tggcacagta cggcacttct aacaccggtc
    74461 gctttaaaac gctgtaccgt gaagggctga aaaatcgcta cggcgcgctg atgcaaacca
    74521 tttccggcgt gcactataat ttctctttgc caatggcatt ctggcaggcg aagtgcggtg
    74581 atatctcggg cgctgatgcc aaagagaaaa tttctgcggg ttatttccgc gttatccgta
    74641 actactatcg cttcggttgg gtcattcctt atctgtttgg tgcgtctcca gcgatttgtt
    74701 cttctttctt gcaaggaaaa ccaacgtcgc tgccgtttga gaaaaccgag tgcggtatgt
    74761 attacctgcc gtatgcgacc tctcttcgtt tgagcgatct cggctatacc aataaatcgc
    74821 aaagcaatct tggtatcacc ttcaatgatc tctacgagta cgtggcgggc cttaaacagg
    74881 caatcaaaac gccatcggaa gagtacgcga agattggtat tgagaaagac ggtaagcggc
    74941 tgcaaatcaa cagcaatgtg ctgcagattg aaaacgaact gtacgcgccg attcgtccaa
    75001 aacgcgttac ccgcagtggt gagtcgcctt ctgatgcgct gttacgtggc ggcattgaat
    75061 atattgaagt ccgctcgctg gacatcaacc cgttctcgcc gattggtgta gatgaacaac
    75121 aggtgcgatt cctcgacctg tttatggtct ggtgtgcgct ggctgacgca ccggaaatga
    75181 gcagtagcga acttgcctgt acacgcgtta actggaaccg ggtgatcctc gaaggtcgca
    75241 aaccgggcct gacgctgggt atcggctgcg aaaccgcgca gttcccgtta ccgcaggtgg
    75301 gtaaagatct gttccgcgat ctgaaacgcg tcgcgcaaac gctggatagc attaacggcg
    75361 gcgaagcgta tcagaaagtg tgtgatgaac tggtcgcctg cttcgataat cccgatctga
    75421 ctttctctgc ccgtatctta aggtctatga ttgatactgg tattggcgga acaggcaaag
    75481 cgtttgctga agcgtaccgt aatctgctgc gtgaagagcc gctggaaatt ctgcgcgaag
    75541 aggattttgt cgccgagcgc gaggcgtctg aacgccgtca gcaggaaatg gaagccgctg
    75601 ataccgaacc gtttgcggtg tggctggaaa agcacgcctg acagaaaaga aaaaggccac
    75661 tcgtgagtgg ccaaaatttc atctctgaat tcagggatga tgataacaaa tgcgcgtctt
    75721 tcatatactc agactcgcct gggaacaaag agttcagaaa attttaaaaa aaattaccgg
    75781 aggtggctaa atgccgttgt tagatagctt cacagtcgat catacccgga tggaagcgcc
    75841 tgcagttcgg gtggcgaaaa caatgaacac cccgcatggc gacgcaatca ccgtgttcga
    75901 tctgcgcttc tgcgtgccga acaaagaagt gatgccagaa agagggatcc ataccctgga
    75961 gcacctgttt gctggtttta tgcgtaacca tcttaacggt aatggcgtag agattatcga
    76021 tatctcgcca atgggctgcc gcaccggttt ttatatgagt ctgattggta cgccagatga
    76081 gcagcgtgtc gctgatgcct ggaaagcggc aatggaagac gtgctgaaag tgcaggatca
    76141 gaatcagatc ccggaactga acgtctacca gtgtggcact taccagatgc actcgttgca
    76201 ggaagcgcag gatattgcgc gtaacattct ggaacgtgac gtgcgcatca acagcaacga
    76261 agaactggcg ctgccgaaag agaagttgca ggaactgcac atttagtcag tttaagcaga
    76321 agaggccggg ctggtgaaaa caccgcccgg cttttgttac ctgtcagtca tccgatattc
    76381 cacgctatgt gcttcatcag gttgaggaac ttccagcctt gaggccatcc gcatcacgac
    76441 cgcttcagga actgcatact cgcgtctggc attttgctgg ttccattgcg cccatggcac
    76501 ttccagataa acgattttta ctcgtgcgcc gtaagccgta aacagactga tgagctggct
    76561 gcgcagctgg cgggtaatgt tcgttgcatt ccagatgaac ggctttttct ggcgtaaaaa
    76621 aacgcgcgcc tcttcttttg cctgttgcac aatacgcccg gtggcggttt tgtcgtccgg
    76681 actggcattg attcgccgcc gcatatcatc aaggctgata acgtccattc cctggcactg
    76741 ttcactgata tagcgatctt tgcccatacc cggtaagcca cacagcaaaa taacctcaaa
    76801 aggttcagcc tcccagggaa caaaatcagg agaactttgc tcatgagtca ggtagtgcca
    76861 gcgtgcggaa tcagaaacga acgggcgcat ttttccccag cattgttgct catggcaaaa
    76921 cagctcgaac agatcgatgc gttccagcat tgattgctga tccgggcttt ggcgaccaag
    76981 cagatcggca cgagcaagca aagcgagcag acgcgtgtcg atgcgcattg cagccgtgag
    77041 caacaaacgc tccggttccg gacgttccag taaccatagc ggtaacccat gcagacgcac
    77101 cagtgcaaca atttgctcgc gcagcacgaa cggcgttggg atatcgcgcc ataaaatttg
    77161 tcgagccgtc agttcgcccc gacgagcatg accaggagac tgaatacgtc cgttttcctg
    77221 cacggtagtg ctgcgctttt caacatcatg caataacgcc gccgcccata aaacttcctg
    77281 ctgttgagcg gggagttgct ggaattctgg cagagtaatg agggcgttaa gcaccatttc
    77341 ggtatgtacg ccgacgtcgc cttcaccatg atgctcaggg tcttgtggcg tgtgatgcat
    77401 ctcctcaacc cagctaaaac gctgacgtag cgccgaccag cgtttatcgt cggtaagttg
    77461 ccaaatcatg actgttctcc ataaacgaac tcatgggcca tccgcgcccg ttgccagtgg
    77521 cgtttccagt gcacagtggt tttgacatga tttttgcgca catacttgaa gacgttatgc
    77581 gaaaagtcag cgacagaaaa cgcatcatga tttcggctga caatgccttc cagcgtacag
    77641 ggttgacagg tttgcgtatc ccagggatcg aatgtgccgc gcgcattggt cagcgccaga
    77701 aaatcacgtt gccagctttt ttcatcattc cccggctgcg ggccggagat ttcgggaacg
    77761 caggggaaat caaacagagc ggcgtaaaac tgcacctctt cccagctcag ccacatatcc
    77821 tggcagcgaa cggcaaacag gtaaaagtcc tgttccagcg cgcggtattc aatggaatga
    77881 acggcgtaga gattttcacc aaacaactcc aggttgccga gatcgttttt cagcagttgc
    77941 cagcgttggc gaattttata ggtccatgcc gactgcgtag gcgtggcatg ggagcgggca
    78001 aacacgccgt agcggttaag gcagttattt tcgccatcga gtttttctgt gtgcaccaat
    78061 tgcgtgatgg tctgtaaatc ctgccagtaa tcggtgttaa tgcggtcatc gctggtggtg
    78121 cctggagaga aggggtaatg ccaggttctc ccgtatttac gttgggtatt cataataatc
    78181 cattattttg tgtgtaataa atgtacgccc gcgagggtgc gagttttggg gttaaaagaa
    78241 aaatgtgagt atcacccgcg aaagcgaggg cgaaggaaaa agagaattaa caacctgagc
    78301 gaatgctcag aaccggctaa tggtgaatgg aagagtttgg ggcggcgaaa agatacaccg
    78361 aatgagtgta taaaacaacc gtttgcagaa tgagcaaata aaaagggagc cgcagctccc
    78421 tttggcaacc ttagtgcgca ccgcctccgc cgccacctgc gccaaatggc ggtttagcaa
    78481 accacaccag ccccagcagg acgaggaata tcccggctga catccagaag atctcattgg
    78541 cggaaataat cagcccctga ttggttatct gctgggcaat ccagcctgat gcctgctgtt
    78601 gcgtcatacc aagcccttcc agttgactgt acatcgcctg agcattcggg ttgaacgggt
    78661 ttaccgactc agtcaactgt gcatggtgca gcgactcgcg gttggtccac atggtcgtgg
    78721 ttatcgacgt gccgatagac cccgccagcg ttcgcgtaaa gttggagagg ctcgatgccg
    78781 ccgccagtcg ttccggtggc aaaccggaca gcgtaatggt ggtcagcggc ataaagaagc
    78841 aggccaccgc aaaaccctgg ataaactgtg gccaggccga cgcgccaaaa tccatacccg
    78901 gttcaaaggt ataggcacgc cagtagaagc agacggcata cataataaag ctgaaggtta
    78961 ccagtcggcg catatccagt ttatgcgcga agcggccgat aatcggcgac aggatcaccg
    79021 gaataatccc taccggcgca gaggccagcc ctgcccacgt cgccgtgtaa ccgtagacct
    79081 cctgcaacaa ctgcggcagc agaacaatcg cgccgaagta gagcatatac gcgaggctga
    79141 tacacaagca gccgatggtg aagttgcgcg acttaaacaa cgacagatcg actatcgggt
    79201 tatcgtcggt cagctcccag acaatcagga agcagatagc caccaccgcc accacggtga
    79261 ggatgataat ttcctgtgat gagaaccagt ccagctcttt accgcggtcg agcataatct
    79321 gcaggctgcc gataccaata accagcagtg ccagccccac ggcatcaatc cgccgccgtt
    79381 cggtgcgggt ttcacgtccg cgcagagttt gcagtgtcat caacaccacc gccacgccaa
    79441 tcggcacgtt gatgaagaat atccagcccc agtggtaatt atcactgata taaccgccga
    79501 ggatcgggcc gcaaattggc gcaacaatca ccgtcatcga ccacaacgcc agcgcgatcg
    79561 agcgtttggc tggcgggtag ttattcagca atagactttg cgaaagcgga attaacggcc
    79621 cggcgacaat cccctgaatc acgcggaaga agatcagcat attcaggctg ctggagacac
    79681 cacacgccca cgacgcaata gcaaaggcga tggtggacca gaggaacagt ttcacttccc
    79741 cgacgcgctt tgccagccag ccggtaagcg ggatcgagat ggcattcgcc accccgaaag
    79801 aagtgattac ccacgttccc tggctgagcg atgagcccag attcccggcg atagtgggga
    79861 tcgccacgtt agcaatggtg gagtccagca cctgcatgaa tgtcgccagt gacagcgcaa
    79921 tcgtcataat gaccagttgt gcgccttcca gcggtttttg ctgttgcatc acacgcacct
    79981 ctggattagc cagcgttagc ttttacgata tcgtcgatca gtttattgac aggtgccagg
    80041 ctgatttcac gcgcggtgct taccgcgacc ggtgtggaac gtactttgtt tgccagtacc
    80101 tgaccgtcac ggttagtggt attgacactc accagcgtgg ataaaccgat acgcagcggg
    80161 tattgctcca gctgtttctg gtccagttca atacgcacag gcagacgctg aacgactttg
    80221 atccagttac cggtggcgtt ttgcgccgga agcagtgaga acgcgctacc tgtgcccata
    80281 tccagaccaa ccactttacc ggtgtatttc acatcatcgc cgtaaatatc ggtggtgatg
    80341 gtgaccggtt gaccgatacg catattggca atctgcgtct ctttaaagtt ggcatccacc
    80401 cacatattgg tggctggaac gaccgccatc agcggcgtcg ttgggctaat ttgcgcccca
    80461 ggctgtaccg cgcggcggga gacataaccg gtcatcggac tgacaatacg ggtacgctcc
    80521 agcgccagcc aggcgttacg tacttcggtg gcagcctgtt gtacggcagg ctgatcttcc
    80581 agtttcgtcc ccagaatcat cgcctgattg gcattgtatt gttgaatagc gacgtccagt
    80641 tgcgcctggg cactggtgac ggcgtcgcgg gcgtgttgca gctcttcgcg accaatcagg
    80701 ttggcattgc ccagcggcac acggcggttg tagtcgcttt gtgctttcgc gagggcgatt
    80761 ttttgcacct caatattcgc ctgcaactgt ttgctgttaa tcatcagctg gtgggtttgg
    80821 cgaacgctgg aagccagtgc agttttggct ttttcaaacg cctggcgagc atctgtcggg
    80881 tcgagagtga ccagcacgtc gccttctttt acaaaatcgg tgttatcggc ccagactttc
    80941 gtcacgctgc cagacacctg agacataatt tgaatctgat tccctgccac gtatgcgtca
    81001 tcggtttctt cgaaatgacg cagtaccaaa aaccaataaa tccctatcgc tacggcaata
    81061 attataaaga gcaaggtgag aaggaggagc agacgcttac gtttgccgct cttctttacc
    81121 ggttgctgcg gggtttgagt ctccgcattt gcgctcatat tgttctccac gatcttctta
    81181 actcatcggc tgagccgacc tgcttgccaa aaaggccagc atgttgcgat gctggccggt
    81241 tattttttct tttataaatc tggatttttg agcgagatga cgcgttagct catcgcttcg
    81301 agaaccacac cgccctgttc catctggtcg agacgggaga gcaatttgcg ggtgatttgc
    81361 tcgagctggt ctttttctgt tgtgctgagc gcggaccaga gttgatgcag gcagttatgc
    81421 tgcggcggta aaacctcgcg caaaaactcg tgaccttttt ccgttaattg cagatgcagg
    81481 cagcggcgat cgttatcgct ttcacgacgt tcgatccaac cgcgtttttc cagttcatcg
    81541 gcaatacgcg tcgcgttggt acgggatgat ccaagagcac aactcaattc agaaggctga
    81601 atactgtggt tttcctgaga ctccagcgta atcaacgcca taaacaacgt ctcgttaatc
    81661 ccctgagcct tcagcatttt attgcggttt tccagcagct tgctttgcat atgcatgcaa
    81721 agacgggtca gaaggatctc ctgataagga aaatcttcgt ggcggctggc gcgaaatttt
    81781 agcatttgtt caatgggcgt aaacgaacta tccatttggg tatgacctca ttaatttcag
    81841 ccgatatagt aacgacagtg acaaataaag taaatgtatt gttttagaaa aatgattctt
    81901 gtggattata taatcgccat cactttccag gcgagcccat aggccagcgc actaagcagt
    81961 gttgggatga taatgctgcg tgttttatag aaactggcac ccagtaccgc gaagccgacc
    82021 agtgtgggca cgaaacggcg tgtatcgtgc atcacttctg gtgcggtaga gacaaccagc
    82081 agagcgcata tcgaggcgat gccaatggtg tcgagcaaaa tacctaccgc gccacgtttg
    82141 gttgggcggg cattacccac acgcaggcgc agcggcagat agcggaagca ataattcgcc
    82201 gcgccaacca gtaacccaag cagcagaacc tcatagctca tcgggcgctc cttgccagaa
    82261 tgcctggatt aacgcagtga ggcagccaca gacaatgcct gccagaatgg cgacgggaat
    82321 agaaaatagc gttacgcctg caagggcacc aactaacgct gcggtaacgc aaagagattg
    82381 tttgcgctgg aaagaggcga gcaggaaact cataaagagt gccggaagca taaaacctaa
    82441 tgctgcttca acggcgggat aaccttgcag caagccgctg ccggagaatg cccctattac
    82501 cgtaccaaat acccacgatg accatgaact gaaggcaatg ccgatcatcc agttttcgct
    82561 ccagcggcga ttattgcgta ccagttttgc ggttgcggcg gcaaaaacct catccgtcag
    82621 gccaaacgcc cacagggcgg ttttcgattt ttgcagac
//
LOCUS       DAOFSV010000005        79673 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.005, whole genome shotgun sequence.
ACCESSION   DAOFSV010000005 DAOFSV010000000
VERSION     DAOFSV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.005"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            99..359
                     /gene="iraP"
                     /locus_tag="QBH27_000375"
     CDS             99..359
                     /gene="iraP"
                     /locus_tag="QBH27_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDQ7012746.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
     gene            460..1875
                     /gene="phoA"
                     /locus_tag="QBH27_000376"
     CDS             460..1875
                     /gene="phoA"
                     /locus_tag="QBH27_000376"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDQ7012747.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            1994..2314
                     /gene="psiF"
                     /locus_tag="QBH27_000377"
     CDS             1994..2314
                     /gene="psiF"
                     /locus_tag="QBH27_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDQ7012748.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2416..3531
                     /gene="adrA"
                     /locus_tag="QBH27_000378"
                     /gene_synonym="dgcC"
     CDS             2416..3531
                     /gene="adrA"
                     /locus_tag="QBH27_000378"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDQ7012749.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3548..4357)
                     /gene="proC"
                     /locus_tag="QBH27_000379"
     CDS             complement(3548..4357)
                     /gene="proC"
                     /locus_tag="QBH27_000379"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDQ7012750.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4477..4935
                     /locus_tag="QBH27_000380"
     CDS             4477..4935
                     /locus_tag="QBH27_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDQ7012751.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5118..5642
                     /gene="aroL"
                     /locus_tag="QBH27_000381"
     CDS             5118..5642
                     /gene="aroL"
                     /locus_tag="QBH27_000381"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDQ7012752.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5692..5883
                     /gene="yaiA"
                     /locus_tag="QBH27_000382"
     CDS             5692..5883
                     /gene="yaiA"
                     /locus_tag="QBH27_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDQ7012753.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6141..6818
                     /locus_tag="QBH27_000383"
     CDS             6141..6818
                     /locus_tag="QBH27_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDQ7012754.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            6890..7174
                     /gene="ppnP"
                     /locus_tag="QBH27_000384"
     CDS             6890..7174
                     /gene="ppnP"
                     /locus_tag="QBH27_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDQ7012755.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7322..7663
                     /locus_tag="QBH27_000385"
                     /pseudo
     CDS             7322..7663
                     /locus_tag="QBH27_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            7660..7740
                     /locus_tag="QBH27_000386"
     CDS             7660..7740
                     /locus_tag="QBH27_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012756.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(7821..8732)
                     /gene="rdgC"
                     /locus_tag="QBH27_000387"
     CDS             complement(7821..8732)
                     /gene="rdgC"
                     /locus_tag="QBH27_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDQ7012757.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQVRKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8857..9765
                     /gene="mak"
                     /locus_tag="QBH27_000388"
     CDS             8857..9765
                     /gene="mak"
                     /locus_tag="QBH27_000388"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDQ7012758.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVHKAKHGDSSGVRGAAWLWPQE"
     gene            complement(10034..11218)
                     /gene="araJ"
                     /locus_tag="QBH27_000389"
     CDS             complement(10034..11218)
                     /gene="araJ"
                     /locus_tag="QBH27_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDQ7012759.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11344..14487)
                     /gene="sbcC"
                     /locus_tag="QBH27_000390"
     CDS             complement(11344..14487)
                     /gene="sbcC"
                     /locus_tag="QBH27_000390"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDQ7012760.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKDEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEQA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASAQAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNKMRHRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(14484..15686)
                     /gene="sbcD"
                     /locus_tag="QBH27_000391"
     CDS             complement(14484..15686)
                     /gene="sbcD"
                     /locus_tag="QBH27_000391"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDQ7012761.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHLVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQLIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            15876..16565
                     /gene="phoB"
                     /locus_tag="QBH27_000392"
     CDS             15876..16565
                     /gene="phoB"
                     /locus_tag="QBH27_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDQ7012762.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16623..17918
                     /gene="phoR"
                     /locus_tag="QBH27_000393"
     CDS             16623..17918
                     /gene="phoR"
                     /locus_tag="QBH27_000393"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDQ7012763.1"
                     /translation="MLERLSWKRLVLELLFCCLPAFILGAFLGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFIIPERLIAKNSD"
     gene            18325..19644
                     /gene="brnQ"
                     /locus_tag="QBH27_000394"
     CDS             18325..19644
                     /gene="brnQ"
                     /locus_tag="QBH27_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDQ7012764.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19720..21093
                     /gene="proY"
                     /locus_tag="QBH27_000395"
     CDS             19720..21093
                     /gene="proY"
                     /locus_tag="QBH27_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDQ7012765.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            21249..23066
                     /gene="malZ"
                     /locus_tag="QBH27_000396"
     CDS             21249..23066
                     /gene="malZ"
                     /locus_tag="QBH27_000396"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDQ7012766.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARQEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23071..23652)
                     /gene="acpH"
                     /locus_tag="QBH27_000397"
     CDS             complement(23071..23652)
                     /gene="acpH"
                     /locus_tag="QBH27_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDQ7012767.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            23745..24815
                     /gene="queA"
                     /locus_tag="QBH27_000398"
     CDS             23745..24815
                     /gene="queA"
                     /locus_tag="QBH27_000398"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDQ7012768.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24870..25997
                     /gene="tgt"
                     /locus_tag="QBH27_000399"
     CDS             24870..25997
                     /gene="tgt"
                     /locus_tag="QBH27_000399"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDQ7012769.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            26020..26352
                     /gene="yajC"
                     /locus_tag="QBH27_000400"
     CDS             26020..26352
                     /gene="yajC"
                     /locus_tag="QBH27_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDQ7012770.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26380..28227
                     /gene="secD"
                     /locus_tag="QBH27_000401"
     CDS             26380..28227
                     /gene="secD"
                     /locus_tag="QBH27_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDQ7012771.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28238..29209
                     /gene="secF"
                     /locus_tag="QBH27_000402"
     CDS             28238..29209
                     /gene="secF"
                     /locus_tag="QBH27_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDQ7012772.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29339..29686
                     /locus_tag="QBH27_000403"
     CDS             29339..29686
                     /locus_tag="QBH27_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDQ7012773.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29724..30608)
                     /gene="tsx"
                     /locus_tag="QBH27_000404"
     CDS             complement(29724..30608)
                     /gene="tsx"
                     /locus_tag="QBH27_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDQ7012774.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30907..31446)
                     /locus_tag="QBH27_000405"
     CDS             complement(30907..31446)
                     /locus_tag="QBH27_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDQ7012775.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            31597..32046
                     /gene="nrdR"
                     /locus_tag="QBH27_000406"
     CDS             31597..32046
                     /gene="nrdR"
                     /locus_tag="QBH27_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDQ7012776.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            32050..33153
                     /gene="ribD"
                     /locus_tag="QBH27_000407"
     CDS             32050..33153
                     /gene="ribD"
                     /locus_tag="QBH27_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDQ7012777.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33242..33712
                     /gene="ribE"
                     /locus_tag="QBH27_000408"
     CDS             33242..33712
                     /gene="ribE"
                     /locus_tag="QBH27_000408"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDQ7012778.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33732..34151
                     /gene="nusB"
                     /locus_tag="QBH27_000409"
     CDS             33732..34151
                     /gene="nusB"
                     /locus_tag="QBH27_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDQ7012779.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34229..35206
                     /gene="thiL"
                     /locus_tag="QBH27_000410"
     CDS             34229..35206
                     /gene="thiL"
                     /locus_tag="QBH27_000410"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDQ7012780.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            35184..35702
                     /gene="pgpA"
                     /locus_tag="QBH27_000411"
     CDS             35184..35702
                     /gene="pgpA"
                     /locus_tag="QBH27_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDQ7012781.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAVILY
                     FIGHHWPLGILS"
     gene            complement(35756..36730)
                     /gene="yajO"
                     /locus_tag="QBH27_000412"
     CDS             complement(35756..36730)
                     /gene="yajO"
                     /locus_tag="QBH27_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDQ7012782.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKVGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(36785..38647)
                     /gene="dxs"
                     /locus_tag="QBH27_000413"
     CDS             complement(36785..38647)
                     /gene="dxs"
                     /locus_tag="QBH27_000413"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDQ7012783.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(38672..39571)
                     /gene="ispA"
                     /locus_tag="QBH27_000414"
     CDS             complement(38672..39571)
                     /gene="ispA"
                     /locus_tag="QBH27_000414"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDQ7012784.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSFDTSALEALADYIIQRNK"
     gene            complement(39571..39813)
                     /gene="xseB"
                     /locus_tag="QBH27_000415"
     CDS             complement(39571..39813)
                     /gene="xseB"
                     /locus_tag="QBH27_000415"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDQ7012785.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            40019..41467
                     /gene="thiI"
                     /locus_tag="QBH27_000416"
     CDS             40019..41467
                     /gene="thiI"
                     /locus_tag="QBH27_000416"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDQ7012786.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     NWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41521..42111)
                     /gene="yajL"
                     /locus_tag="QBH27_000417"
     CDS             complement(41521..42111)
                     /gene="yajL"
                     /locus_tag="QBH27_000417"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDQ7012787.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(42074..42985)
                     /gene="panE"
                     /locus_tag="QBH27_000418"
     CDS             complement(42074..42985)
                     /gene="panE"
                     /locus_tag="QBH27_000418"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDQ7012788.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43153..43644
                     /gene="yajQ"
                     /locus_tag="QBH27_000419"
     CDS             43153..43644
                     /gene="yajQ"
                     /locus_tag="QBH27_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDQ7012789.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43772..45136)
                     /locus_tag="QBH27_000420"
     CDS             complement(43772..45136)
                     /locus_tag="QBH27_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7012790.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHVLFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(45285..46175)
                     /gene="cyoE"
                     /locus_tag="QBH27_000421"
     CDS             complement(45285..46175)
                     /gene="cyoE"
                     /locus_tag="QBH27_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDQ7012791.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(46187..46516)
                     /locus_tag="QBH27_000422"
     CDS             complement(46187..46516)
                     /locus_tag="QBH27_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDQ7012792.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(46516..47130)
                     /locus_tag="QBH27_000423"
     CDS             complement(46516..47130)
                     /locus_tag="QBH27_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDQ7012793.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(47120..49111)
                     /gene="cyoB"
                     /locus_tag="QBH27_000424"
     CDS             complement(47120..49111)
                     /gene="cyoB"
                     /locus_tag="QBH27_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDQ7012794.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQIYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(49133..50080)
                     /gene="cyoA"
                     /locus_tag="QBH27_000425"
     CDS             complement(49133..50080)
                     /gene="cyoA"
                     /locus_tag="QBH27_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDQ7012795.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(50540..52015)
                     /gene="ampG"
                     /locus_tag="QBH27_000426"
     CDS             complement(50540..52015)
                     /gene="ampG"
                     /locus_tag="QBH27_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDQ7012796.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(52059..52637)
                     /locus_tag="QBH27_000427"
     CDS             complement(52059..52637)
                     /locus_tag="QBH27_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ7012797.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            52942..53259
                     /gene="bolA"
                     /locus_tag="QBH27_000428"
     CDS             52942..53259
                     /gene="bolA"
                     /locus_tag="QBH27_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDQ7012798.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            53603..54901
                     /gene="tig"
                     /locus_tag="QBH27_000429"
     CDS             53603..54901
                     /gene="tig"
                     /locus_tag="QBH27_000429"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDQ7012799.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            55147..55770
                     /gene="clpP"
                     /locus_tag="QBH27_000430"
     CDS             55147..55770
                     /gene="clpP"
                     /locus_tag="QBH27_000430"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDQ7012800.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            55896..57170
                     /gene="clpX"
                     /locus_tag="QBH27_000431"
     CDS             55896..57170
                     /gene="clpX"
                     /locus_tag="QBH27_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDQ7012801.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIANRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            57358..59712
                     /gene="lon"
                     /locus_tag="QBH27_000432"
     CDS             57358..59712
                     /gene="lon"
                     /locus_tag="QBH27_000432"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDQ7012802.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            59921..60193
                     /gene="hupB"
                     /locus_tag="QBH27_000433"
     CDS             59921..60193
                     /gene="hupB"
                     /locus_tag="QBH27_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDQ7012803.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            60385..62256
                     /gene="ppiD"
                     /locus_tag="QBH27_000434"
     CDS             60385..62256
                     /gene="ppiD"
                     /locus_tag="QBH27_000434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDQ7012804.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREAAIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            62407..62778
                     /locus_tag="QBH27_000435"
     CDS             62407..62778
                     /locus_tag="QBH27_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDQ7012805.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNMAVLTL"
     gene            62896..63294
                     /gene="fadM"
                     /locus_tag="QBH27_000436"
     CDS             62896..63294
                     /gene="fadM"
                     /locus_tag="QBH27_000436"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDQ7012806.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(63346..64041)
                     /gene="queC"
                     /locus_tag="QBH27_000437"
     CDS             complement(63346..64041)
                     /gene="queC"
                     /locus_tag="QBH27_000437"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDQ7012807.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(64106..65806)
                     /locus_tag="QBH27_000438"
     CDS             complement(64106..65806)
                     /locus_tag="QBH27_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDQ7012808.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65906..66724
                     /gene="cof"
                     /locus_tag="QBH27_000439"
     CDS             65906..66724
                     /gene="cof"
                     /locus_tag="QBH27_000439"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDQ7012809.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            66877..67335
                     /gene="decR"
                     /locus_tag="QBH27_000440"
     CDS             66877..67335
                     /gene="decR"
                     /locus_tag="QBH27_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDQ7012810.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            67365..69137
                     /locus_tag="QBH27_000441"
     CDS             67365..69137
                     /locus_tag="QBH27_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDQ7012811.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            69130..70911
                     /locus_tag="QBH27_000442"
     CDS             69130..70911
                     /locus_tag="QBH27_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDQ7012812.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     SLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            71092..71430
                     /gene="glnK"
                     /locus_tag="QBH27_000443"
     CDS             71092..71430
                     /gene="glnK"
                     /locus_tag="QBH27_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDQ7012813.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            71460..72746
                     /gene="amtB"
                     /locus_tag="QBH27_000444"
     CDS             71460..72746
                     /gene="amtB"
                     /locus_tag="QBH27_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDQ7012814.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(72795..73655)
                     /gene="tesB"
                     /locus_tag="QBH27_000445"
     CDS             complement(72795..73655)
                     /gene="tesB"
                     /locus_tag="QBH27_000445"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDQ7012815.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            73873..74445
                     /locus_tag="QBH27_000446"
     CDS             73873..74445
                     /locus_tag="QBH27_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDQ7012816.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(74476..74787)
                     /locus_tag="QBH27_000447"
     CDS             complement(74476..74787)
                     /locus_tag="QBH27_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDQ7012817.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            74950..75046
                     /gene="ffs"
                     /locus_tag="QBH27_000448"
     ncRNA           74950..75046
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="QBH27_000448"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            75166..75519
                     /locus_tag="QBH27_000449"
     CDS             75166..75519
                     /locus_tag="QBH27_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HDQ7012818.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DIPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(75561..77111)
                     /gene="pdeB"
                     /locus_tag="QBH27_000450"
     CDS             complement(75561..77111)
                     /gene="pdeB"
                     /locus_tag="QBH27_000450"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDQ7012819.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWYRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(77275..77745)
                     /locus_tag="QBH27_000451"
     CDS             complement(77275..77745)
                     /locus_tag="QBH27_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDQ7012820.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(77861..78412)
                     /gene="maa"
                     /locus_tag="QBH27_000452"
     CDS             complement(77861..78412)
                     /gene="maa"
                     /locus_tag="QBH27_000452"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDQ7012821.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(78586..78804)
                     /gene="hha"
                     /locus_tag="QBH27_000453"
     CDS             complement(78586..78804)
                     /gene="hha"
                     /locus_tag="QBH27_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDQ7012822.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(78830..79204)
                     /gene="tomB"
                     /locus_tag="QBH27_000454"
     CDS             complement(78830..79204)
                     /gene="tomB"
                     /locus_tag="QBH27_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDQ7012823.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        19375 a        19702 c        21312 g        19284 t
ORIGIN      
        1 aaagtatttc ctttgtcata aaaataatac ttccagacac tatgaagttg tgaaacataa
       61 tgttaacttc tccatacttt ggataaggaa atacagacat gaaaaatctc attgctgagt
      121 tgttatttaa gcttgcccaa aaagaagaag agtcgaaaga actgtgtgcg caggtagaag
      181 ctttggagat tatcgtcacg gcaatgcttc gcaatatggc gcaaaatgat caacagcggt
      241 tgattgatca ggtagagggg gcgctgtacg aggtaaagcc cgatgccagc attcctgacg
      301 acgatacgga gctgctgcgc gattacgtaa agaagttatt gaggcatcct cgtcagtaaa
      361 aagttaatct tttcaacagc tgtcataaag ttgtcacggc cgagacttat agtcgctttg
      421 tttttatttt ttaatgtatt tgtacatgga gaaaataaag tgaaacaaag cactattgca
      481 ctggcactct taccgttact gtttacccct gtgacaaaag cccggacacc agaaatgcct
      541 gttctggaaa accgggcagc tcagggcgat attactgcac ccggcggtgc tcgccgctta
      601 acgggtgatc agaccgctgc tctgcgtgat tctcttagcg ataaacctgc aaaaaatatt
      661 attttgctga ttggcgatgg gatgggggac tcggaaatta ctgccgcacg caattatgcc
      721 gaaggtgcgg gcggcttttt taaaggtatc gatgccttac cgcttaccgg gcaatacact
      781 cactatgcgc tgaataaaaa aaccggcaaa ccggactacg tcaccgactc ggctgcatca
      841 gcaaccgcct ggtcaactgg tgtcaaaacc tataacggcg cgctgggcgt cgatattcac
      901 gaaaaagatc acccaacgat tctggaaatg gcaaaagccg caggtctggc gaccggtaac
      961 gtttctaccg cagagttgca ggatgccacg cccgctgcgc tggtggcgca tgtgacctcg
     1021 cgcaaatgct acggtccaag tgcgaccagt gaaaaatgtc cgggtaacgc tctggaaaaa
     1081 ggcggaaaag gatcgattac cgaacagctg cttaacgccc gtgccgatgt tacgcttggc
     1141 ggcggtgcaa aaacctttgc tgaaacggca accgccggtg aatggcaggg aaaaacgctg
     1201 cgtgaacagg cacaggcgcg tggttatcag ttggtgagcg atgctgcctc actgaattcg
     1261 gtgacggaag cgaatcagca aaaaccccta ttaggactgt ttgctgacgg caatatgcca
     1321 gtgcgctggc aaggaccgaa agcaacgtac cacggtaata tagataagcc cgcagtcacc
     1381 tgtacgccta atccgcaacg taatgacagt gtaccgaccc tggcgcagat gaccgacaaa
     1441 gccattgaat tgttgagtaa aaatgagaaa ggctttttcc tgcaagttga aggtgcgtca
     1501 atcgataaac aggatcacgc tgcgaatcct tgtgggcaaa ttggcgagac ggtcgatctc
     1561 gatgaagccg tacaacgggc gctggaattc gctaaaaagg atggtaacac gttggtcata
     1621 gtcaccgctg atcacgccca tgccagccag atagttgcgc cagataccaa agctccgggc
     1681 ctcacccagg cgctaaatac caaagatggc gcagtgatgg tgatgagtta cgggaactcc
     1741 gaagaggatt cacaagaaca taccggcagt cagttgcgta ttgcggcgta tggcccgcat
     1801 gccgccaatg ttgttggact gaccgaccag accgatctct tctacaccat gaaagccgcc
     1861 ctggggctga aataaaaccg cgcccggcag tgaattttcg ctgccgggtg gtttttttgc
     1921 tgttagcaac cagacttaat gacagatcac gggcgcatac gctcatggtt aaaacatgaa
     1981 gagggatggt gcgatgaaaa ttacattact ggttaccttg cttttcggtc tggttttttt
     2041 aaccaccgtc ggcgctgccg agagaacctt aaccccacaa caacagcgta tgacctcttg
     2101 taatcagcag gcgacggcgc aggcgttgaa aggggatgct cgtaagacct acatgagtga
     2161 ttgcctgaag aacagcaaat ctgcgcctgg tgaaaaaagt ttgacgccac agcagcaaaa
     2221 gatgcgcgaa tgtaataatc aagcaacaca acaatctctg aaaggggatg atcgtaataa
     2281 gtttatgagt gcctgcctca agaaagccgc ctgatacctg atagtgctaa cgggtgagct
     2341 acgaaaatgg ctcacccgaa atttcatact tctgccttta gtcccgtctc tataatttgg
     2401 gaaaattgtt tctgaatgtt cccaaaaata atgaatgatg aaaacttttt caaaaaagcg
     2461 gcggcgcatg gggaggaacc tcctttaact cctcaaaacg aacatcagcg gtccgggctg
     2521 cgcttcgccc gtcgcgtcag actaccccgc gcggttggtc tggctggcat gttcttaccg
     2581 attgcttcaa cgctggtttc gcacccgccg ccgggctggt ggtggctggt gttggtcggc
     2641 tgggcgttcg tctggccgca tttagcctgg cagatagcga gcagggccgt cgatccgctt
     2701 agccgggaaa tttacaactt aaaaaccgat gcagtattag cgggaatgtg ggtaggcgta
     2761 atgggcgtaa acgtgctgcc ttccaccgcg atgttgatga ttatgtgtct gaatttgatg
     2821 ggggcaggcg gcccccgtct gtttgttgcg ggtctggtgt tgatggtggt ttcctgcctt
     2881 gtcaccctcg aactgacggg catcaccgtg tcgttcaata gcgcaccgct ggaatggtgg
     2941 ctctcccttc ccattattgt catttatcct ctgctgtttg gctgggtcag ctaccagacg
     3001 gcgactaaac tggcggaaca taaacgcagg ttgcaggtca tgagtacccg cgacggtatg
     3061 acgggcgtgt ataacagacg tcattgggaa actatgttac gcaatgaatt tgataactgt
     3121 cggcggcata atcgcgatgc aacgttactg attatcgata tcgaccattt caagagcatc
     3181 aacgatacct ggggccatga tgtgggtgat gaagcgattg tggcgcttac ccgacagtta
     3241 caaattaccc tgcgcggtag cgatgtgatt ggtcggtttg gcggcgatga gtttgcagta
     3301 atcatgtccg gtacgccagc tgagagcgcc atcaccgcca tgttacgggt gcatgaaggg
     3361 ctaaacacgt tacgcttgcc gaatacgcca caggtaactt tacggattag tgtgggggtt
     3421 gcgccgctga atccacaaat gagtcactat cgcgagtggt tgaaatcggc agatttggcg
     3481 ctctacaaag caaagaaagc cggacgtaac cgcaccgaag tggcggcctg acgtccggca
     3541 aaagccatca ggatttgctg agtttttctg atttttccat acacttcgtc atcgcttcga
     3601 tcactgcggc acggaagcct ttctcttcca gtacgcgtac cgcctcaatg gtggtgcctc
     3661 ccggtgagca gaccatatct ttcagtgttc ccggatgttc tcccgtttcc agcaccattt
     3721 ttgcggagcc cattaccgcc tgagcggcaa atttatacgc ctgggcgcgt ggcatcccgc
     3781 ccagcacggc ggcgtcggcc atcgcttcga taaacataaa gacgtaggct ggcgaagaac
     3841 cgctcacacc gaccaccggg tggatcatcg gctcagcaat cacttccgct tcgccaaagc
     3901 agcggaaaat attcagcaca tcagcggtat cttctggggt taccagcgcg tttggcgtta
     3961 cggaggtcat cccggcatta accagcgcgg gagtgttcgg catggcgcgg ataattttcc
     4021 ggtcatggcc cagcgcgcgg gcaagctggt cgagcgtgat acctgcagca atagaaacga
     4081 ccagagagtc tttattcagg ctggaggtga tttcgctaag cactttaatc atgatgcctg
     4141 gtttaacggc ggcaaaaatg atgtcggcga tttgcgctac ttcttgcgcc gattctgcgg
     4201 cgttgatgcc gaactgttca tgcagggcgg tgactttatc cggggagggg gtatataccc
     4261 agatttgccc tggaagcacc tgaccgctgg caatcagacc gccgagaatg gcttttccca
     4321 tattgccgca gccaataaaa ccgattttct tttccattgc ctcactcctg ccgtgaaatt
     4381 cattgttttg ataatcgctg gcagaagcat aaacagaact atgccggaag gcaaaagcgc
     4441 gacacaatag aggattaccc aacaaaggat gactttatga caatttgggt ggatgccgac
     4501 gcgtgtccca atgtaattaa agagattttg tatcgcgcgg cggaacgtat gcagatgccg
     4561 ctggtactgg tagcaaacca gagtttacgc gtgccgccat cacgatttat tcgtacgctg
     4621 cgcgtcgcgg caggtttcga cgttgccgat aacgaaattg ttcggcagtg tgaagcgggc
     4681 gatttggtga tcaccgcaga tatacctttg gctgctgaag ccattgagaa aggcgcggcg
     4741 gcgcttaatc cgcgcggcga acgttatacg ccagcgacca ttcgtgagcg cctgacgatg
     4801 cgcgatttta tggatacctt acgtgccagt gggatccaga ccggaggacc agatagtctt
     4861 tcacaacgtg accgccaggc ttttgctgcg gagctggaga agtggtggct ggaagtgcaa
     4921 cgtagtcgtg gctaaatgta atttattatt tacacttcat tcttgaatat ttattggtat
     4981 agtaagggat gtattgagat tttcacttta agtggaattt tttctttaca atcgaaattg
     5041 tactagtttg atggtatgat cgccattctc atgacaccgg ctttcgccgc attgcgacct
     5101 attggggaaa acccacgatg acacaacctc tttttctgat cgggcctcgg ggctgtggta
     5161 aaacaacggt cggaatggcc cttgccgatt cgcttaaccg tcggtttgtc gataccgacc
     5221 agtggttgca atcacagctc aatatgacgg tcgcggagat cgtcgaaagg gaagagtggg
     5281 cgggattccg cgccagagaa acggcggcac tggaagcggt aactgcgcca tccaccgtta
     5341 tcgccacggg cggcggcatt attctgacgg aatttaatcg tcacttcatg caaaataacg
     5401 ggatcgtggt ttatttgtgt gcgccagtat cagtcctggt taaccgactg caagctgcac
     5461 cggaagaaga tttacggcca accttaacgg gaaaaccact gagcgaagaa gttcaggaag
     5521 tgctggaaga acgcgatgcg ctatatcgcg aagttgcgca tattatcatc gacgcaacaa
     5581 acgaacccag ccaggtgatt tctgaaattc gcagcgctct ggcacagacg atcaattgtt
     5641 gattttcgag cgcctatact taacgttcat cccgtgaaat aaggaagaac gatgccaacg
     5701 aaaccgcctt atcctcgtga agcatatata gtgacgattg aaaaaggaaa gccaggacag
     5761 acggtaacct ggtaccaact cagagccgat catcctaaac cagactcgtt gatcagtgaa
     5821 catccgaccg ctcaggaagc gatggatgcg aaaaaacgct atgaggaccc tgacaaagag
     5881 tgaccgcatc agaccgctcg gaagggattc tgagtgccgc cacaagggat ctgcgtcaca
     5941 tttttcacaa ttcatgtttt tcaaataatt agaatattaa acaataacaa tccattactg
     6001 gaatcatttg gaatctttac attatgcagt gcaggtctgc tgctacgctt tttgtcattt
     6061 gtagcacaag taagtgtcag cagtggtgct taacacttgc ccggtaatta acgacgaaat
     6121 aaaagtaagg tggaagaaca atgagtgcgt cgttggcgat cctgactatc ggcattgtac
     6181 ctatgcagga agttttgccg ctcctgacag aatacattga cgaagataat atttcccatc
     6241 atagcctgct ggggaagtta agccgcgaag aagtgatggc ggagtacgcg ccagaagcag
     6301 gcgaagacac aattctcaca ttattaaatg acaaccatct ggcccatgtt tcgcgtcgca
     6361 aagttgagcg agacctgcaa ggtgtggttg aagtgctcga taatcagggt tacgacgtca
     6421 ttatattaat gagtacagca aacattagta gtatgaccgc gcgtaatacg atctttcttg
     6481 agccgtcgcg gatattgcca ccgctggttt cctctattgt tgaagatcat caggtggggg
     6541 ttatcgtacc ggttgaggag ttgctgaccg ttcaggcgca aaaatggcaa attttgcaga
     6601 aaccgccagt attttcattg ggtaacccca ttcatgattc ggaacaaaaa atcattgatg
     6661 ccgggaaaga attactggca aaaggtgcag atgtcatcat gctggattgt ttgggattta
     6721 accagcgtca tcgcgattta ctgcaaaaac agctcgatgt tcctgtcttg ctctctaacg
     6781 tattgattgc acggctggct gcggaattac tgatgtaatt ttgcgtgaca gccagcgcct
     6841 ctggccccta tagtgaagta gatgttcaac taccagacag ggccagttta tgcttcaaag
     6901 taatgagtac ttttccggca aagtgaaatc gatcggcttt tccagcagca gcactggtcg
     6961 cgccagcgtg ggtgttatgg ttgaaggtga atacaccttc agcaccgctg agcctgaaga
     7021 gatgacggta atcagtggcg cgctgaatgt gttactgcct gacgcgaccg actggcaagt
     7081 gtatgaagcc ggttcggtgt ttaatgttcc cggtcacagc gagtttcatc tgcaagttgc
     7141 cgaacccacc tcttatctgt gccgctatct gtaattcctc gccttcccct tgaacgggag
     7201 ggcatttttc tgaaatgtcc tctctttaac ccataataat atttcctttg ctgggatttt
     7261 ttcgatttcc gatatattca taattcatca aggttgatat caatatcagc gaagatctcc
     7321 agatattgtt gcggaactgg cgacgataaa agataaatca gatgatgaat ggtggcgtgc
     7381 attgctgcaa agtcgcaatg atcatttgcg ccaaacggca ttgcacaacg cacatacccc
     7441 ggtgttgttg ttaacgacat tgactgagcc tcaggagcgg tcgctggcta ttaataattc
     7501 acagctggct gccgatgtga aaacggcgtg gttaaaagag gatccatcat tactcttatt
     7561 tgtcgaacaa cccgatcttt cgctgttacg tgatttagtg aaaaccgggg caacgcgaaa
     7621 aattcgcagt gaagcgcgtc accggcttga ggaaaaacaa tgacacaacg cccctggagc
     7681 aagttacagc ataaaacgca taatattgct gcgctaagaa ttattgcccg ctgtagctaa
     7741 cgattatatt cctggtgtgg cttcgtacgc cggataagac gcggcaggcg tcgcatccgg
     7801 cattaaaggt aaatcagcaa ttaacgttgt gcttcgccac ctaatccttc aatcaggttt
     7861 tgaattaacg ctgccagttc accagtcatc aggatgaaat cggcatcaaa gcgctgggcg
     7921 aaatcttcac ggtcgatatc ttcgttttga tcgcgcagct cgtcgcagaa cttcagacgc
     7981 ttgagcgaac catcgtcgca catcacaaac tgaatgcgct gctgccagtc gagcgccagt
     8041 ttagtcacca cttttccggc ttcaatgtgg ttggtgatct cttcgctggt cagatcttgt
     8101 ttcttcgcgc ggatcacgcc gccatcttcc agcaacgatt tcagctcggc ttcatcaagc
     8161 agctggaagc cctgtgccgc gctgccggaa cgtacccatt cggtcagcgt cagttcaatc
     8221 gggttttcca tgctcaatgg tacaaccggt aacgacccca ggcttttacg cagcagtgcc
     8281 agcgtatctt ccgctttttt ggcgctggcg cagtccacca taatcaaacc gttaaccgta
     8341 tcgatccaca tcattgtctg gctaaaacgg ctgaaagcac gcggcagcag agagtgcagc
     8401 acttcgtctt tcagcgaatc tttttcggtt ttcttcagct tacgcacctg ttccgcttcc
     8461 agtttggcga ttttcgcttc cagcgcctgt ttaatcaccg gggacgggag gattttttct
     8521 tctttgcgcg cacagataac aatttgacca ttggcaacgt gcgttaacgc atcgctgtgc
     8581 gatcccatcg gaggtaccca gcccatcttc gccatgtcct ggctgccgca tggggtaaat
     8641 gccatcgagg ctagctgttt ttccatctct tctgcacgca gcgaaatctc gcggctaaga
     8701 cggtaaacca ttaaattttt gaaccacagc atgataattt ccacggcctt gtcgttaaat
     8761 ttagcgggca tgataacgaa ttgtcggcgg ccttgcattg ccaatccggt tgtccgtctc
     8821 tacgctattg atattgaaaa aaataaggag agtaccgtgc gtataggtat cgatttaggc
     8881 ggcaccaaaa ctgaagtgat tgcattgggc gatgcagggg agcagttgta ccgccatcgt
     8941 ctgcccacgc cgcgtgatga ttaccggcag actattgaaa cgatcgccac gttggttgat
     9001 atggcggagc aggcaacagg gcagcgcgga acggtaggta tgggcattcc aggttcgatt
     9061 tcgccctaca ccggcgtggt gaagaatgcc aattcaacct ggctcaacgg tcagccattc
     9121 gataaagact taagcgcgcg gttgcagcgg gaagtgcggc tggcaaatga cgccaactgt
     9181 ctggcggttt cagaagcggt agatggcgcg gcagcgggag cgcagacggt ttttgccgtg
     9241 attatcggca cgggatgcgg cgcgggcgtg gcattcaatg ggcgggcgca tatcggcggc
     9301 aatggcacgg caggcgagtg gggacacaat ccgctaccgt ggatggacga agacgaactg
     9361 cgttatcgcg aggaagtccc ttgttattgc ggtaaacaag gttgtattga aacctttatt
     9421 tcaggcacgg gattcgcgac ggattatcgt cgtttgagcg gacatgcgct gaaaggcagt
     9481 gaaattatcc gcctggttga agaaagcgat ccggtagcgg aactggcatt gcgtcgctac
     9541 gagctgcggc tggcaaaatc gctggcacat gtcgtgaata ttctcgatcc ggatgtgatt
     9601 gtcctgggcg gcggaatgag caatgtagac cgtttatatc aaacggttcc gcagttgatt
     9661 aaacaatttg tcttcggcgg agaatgtgaa acgccggtac acaaggcgaa acatggtgat
     9721 tcgagcggcg tacgcggcgc tgcatggtta tggccacaag agtaaaaaac ccaggcaatt
     9781 ggcgtatcat gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa gtgagccgtc
     9841 cggtaggccg gataaggcgc tcgcgccgca tccggcaaca aataacgcgc acagtggcgg
     9901 caaccccccg gtgctccgat gcctgatgcg acactggcgt gtcttatcag gcctacaaaa
     9961 tgaacagtcc cgtaggccgg ataaggcgtt cacgccgcat ccggcaataa gcaacgtgca
    10021 ccgtgactgc aatctacccc agtggtttcg ccagcaccgg actatccgcc gcttgctggc
    10081 gcttatagcg accatacagc agcaacgacg acatcgcagc aaacgaaagc agggcggcgg
    10141 gcagcgccac gtaattatat gccagcccca gcgtcagcat cataccaccg caatatgcgc
    10201 cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat aactctccgc
    10261 ctttggcatt ttgcagtaat aatatttgta gaggtgctga aagggcaaat aatcccgcgc
    10321 aacaaataaa agcaaaaata agcgacgttg ttttcatacc gccgaagaaa aagagcatca
    10381 gcagtgccag tacaatgata aagtcagtca ctgctgcaat gcgcagtggt gaataacgtc
    10441 ctgaaatcct gccacttagc atattcccca gcaccatccc tagcccaact aacatcataa
    10501 taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc tttacgtagc
    10561 tgaaccaggc aaacacacct gcgttgccaa acatcgtagc agcgaaaatt aaccacgggg
    10621 cagggctgcg taaaaagtga aattgttcgc gtagttttcc tttcgcctcg tcgcgaatat
    10681 ccggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaatagcg atcaacaaaa
    10741 aggtgtaacg ccagctaaat tcctgactta aatacgttcc cagtggaatg cccagcaaat
    10801 tggcgactgt catcccggaa accatccctg ccacggcggc ggtgactttg ccgggtttga
    10861 taatttttga taacacgatc gcgccgacgc caaaaaatgc accatgcggg aagccggaaa
    10921 ccagccgacc aatggcgagc atcaggtaag acgaagagag cgtgaacatg gcgttgccaa
    10981 tgacgcacaa cgccaccaga aacaacaaga tatgtttgag tgagtagcga ctggaaaaaa
    11041 gcgcgatgat tggcgcaccg accaccaccc ccagcgcata atacgagatc atatgcccgg
    11101 cggcaggaat cgaaattcct acgttatgcg ccagctccgt gagcacgccc ataatgccaa
    11161 attcggccat ccccaaacca aacgtgccca gagccaaaga taaaataact tttttcatac
    11221 caccatcaca accaccagaa cggctaccaa tcaccgtagc caataaagtg atagcttgca
    11281 ggataacgac cagtcgggcc aagagataat tcgcccctct gtattcatta tcctgctgaa
    11341 tagttatttc actgcaaacg cactttccag tttgctgtag cccagtccat tgatcttctt
    11401 cactttgatc tgcaccggaa tacgctcttt catcgcttca acgtggctaa tcaccccgat
    11461 ggttttgcca ctggcattca gggcatccaa cgcatcaagg gcggtatcca gcgtttcgct
    11521 atccagcgtg ccaaaacctt catcaaggaa cagcgagtca atacgcgttt tatggctgac
    11581 cagatctgaa agcgccagtg ccagcgccag actgacgagg aaactttcgc cgccggaaag
    11641 ggtacgggta tcgcgtaccg catcagcctg ccaggtatca acaacctcaa cttccagcgc
    11701 ctcgctggct ttgcgctgta acagatagcg cccgtgcagc cgggtaagtt gctgattagc
    11761 gagatggact aaattatcca gcgtcagccc ttgggcaaat ttgcggaatt tatcgccctc
    11821 tttggaacct attagcgaat tcagatatcc ccagtcctca acctgctgcg tcatttgagc
    11881 aatttgctgc attaaggttt gttgttgctg acggttatct gcatcctgct tcagctgctg
    11941 gcgaatctcg ccttgactcg tggtgttttc acgcaacttt tggtgagttt gcgctaactc
    12001 ttgctgaatc tgctccaccg tcacagtgag agacaacccg ccaggtcggt gttgctgatg
    12061 ctgtgtcagc gtttctgctg tctgagtgac cagcgtttgc gcctgacggc gctggttttc
    12121 cagattctgc ttgagctgtt ccagctgcgt tagtgtttgt tcatccatta gcgccgcaag
    12181 gaacgcctgc tgatcgtcaa agacgctcgc ctgtagcgcg gtgtcaaact gcgcctgggc
    12241 tttttgcaga ctttgcgccg ccagaacatc ctgttgctgt aacgtctgct gctggctgtg
    12301 taatgcgaga cattgttcat gaacctgccg ccagttttcc agcgccacag tttcttcgct
    12361 gtgcgggagt tcatcacttt gcggcaacgt ttccagaatc ggcgtcagct gctgcatacg
    12421 gttttgcagc gcggttaatt cgttctggcg ttgctgccag ctctgcgctt cttgctgacg
    12481 cgtcgccaac cagctctctt cttcatcttc ctgtggcaat gtcagtgcat aacccgccaa
    12541 tgccgttaag agctgctgct ggcgttgttc aatttgctgt tgatactgga taatttgctg
    12601 attatgcgcg gcaatctgcc cttgtaattc atgccgttgg ctgagtaacc gcagctggcg
    12661 ttcgtgctca tcttgtgcat ccagccacgg ttgaatatcg tcctgtggct gcagggtgat
    12721 attgaggctg gccgtgacgg cttgccattg ttgagtaagt gcttgctcat cttgtcggag
    12781 gctttgcgct tcgttttcat cgcgctgaag ttgctttgtt aaggcgtcca gttgcccacg
    12841 tagcgtcgca ccttcttcgc cgagcttttt cacttctttt tccagcgtta atagccgggc
    12901 ctgattaacg cccggttcca gcgcctgata cgcctcgacc gccgggtggc tggtggaacc
    12961 acaaagtggg caaggctgac ccgcctgtaa ctgtgcgcgc tgggcttcca gcgttttaat
    13021 gcgcgcttcc tgctcgcaaa tggttttcac atcggcaagt tgttgcatct tttctttata
    13081 acgatggcgc attttgttaa gtgcggcatt acgttgcgtc tgctccagag tgacgttctg
    13141 gatagcgacc tgtaactgcg ccagacgttt ttgttgggga acaatctgcc catgcagtgc
    13201 gaccaggcgc tgacgcagtg ggcgttgctc agcatgttgc gcctgggcgc tagcaacttc
    13261 atcggcggtt aacgtcaacg tgatcgccgc aagcgcatta agtttttgct cagcatgggt
    13321 taactgttgc tgccattgcc gcagatgctc gcgatcgctg gtttgttggg agaactgcgc
    13381 acgccaaccc gccagttcgt tgttccactg acggaagcgg tcgtgttcct gtaaccaggc
    13441 attcaggctt tgttgctgct gctgtaattc tgctgactgc ttcgccgcgt ggtggcgaat
    13501 gctcgcacga agcgccattg tgctctgtaa gcgagtattt acttcttcaa tctgctggcg
    13561 agtatgcgcc agcgccgtgc tgtgttctgc gatgcgttcc cagtgcggac gaagatttcg
    13621 tgccggttgt gccagactga gcgccgccag ttgaggttgc gcttgttctt cttcggctaa
    13681 tgcctgttgc aaggcctgtt gacggcggct ggcttcttgc tgcaattcgt ccagacgcgt
    13741 taaccagttt agcgattgtt gttcttgctg ctgcgcggta attaactgtt tttcttcgtc
    13801 agtaagtacc tgcaaactcg ctgtcagcga ttgcacttgt tccggcgtga gcaacgcgac
    13861 gccgctggcc tgcgcttgca gcttctccag ctctgtgcgg gccgatttgt gctgctcaaa
    13921 aaccatcgcc gagatttgcc cgtagatttc agtgccggtt aactcctcga gcaattctgc
    13981 gcgttctttg ggtttggcat tcaggaaggc agcaaattgc ccctgcgaaa gcagcatcga
    14041 acgggtgaag cgcccgtaat ccagtccggt taacgtcgcc gtcagttcca gcttatcttt
    14101 cactttgtcg gcgagaattt tgccgtctgc acagcgcgcc agctctacgc gtggcacctg
    14161 caaattaccg tccggctggt tacgcgcccg attctggctc cagaacgcgc gatacgcttc
    14221 atctttgact tcaaactcca cttccgccag acattcggcg gtatcgcggg tcatgagatc
    14281 attttgcgat tgtgaaacgt tagagaggcg cggagtttcg tgatacagcg ccagacaaat
    14341 ggcgtccagc agggtggttt tccccgcacc ggttgggccg gtaatagcaa acagcccgtt
    14401 gctggcgaac ggctcgcggg tgaaatcaat cttccattcg ccttttaatg agttcaggtt
    14461 tttcaggcgc aggctgagaa ttttcatgct tcgtgttctc cggcgaggct atgcaacgtc
    14521 gtggcgaaaa gatgctgcag acgttgctgc tgtgattcat ccagttcttc cagtgccaga
    14581 cggcgattga acacctcttc gacgctgagt tcgctgaggg tttcacgctg ttggctggct
    14641 aacacacgct cgcgctgttc acgactccgg cgtaccagca atacttcgac aggcaatgat
    14701 tcggttaatg cctggatttt gcgctgaata tcatgcagat actcatcagt agtgatttcg
    14761 atatccagcc agacaggcgg ctcctgtgat acatcgcgcc actgttccag ctgtgcggta
    14821 atcgacgcca gatcgccttt cagcaccgcc atgggttgcg ttaccggtac gttcaggttt
    14881 tccacgctct ctaatttgcc gtttgaaaat gtcaccagat ggacatattt actcttaccg
    14941 cattcatcaa aactcagtgg aatgggggag ccgcaatagc gaacatgttc catgccgcca
    15001 ataagctgcg cgcggtgaat atgcccgagc gcgatgtagt cggctggtgg aaagttttgt
    15061 gccggaaacg cgtccagcgt gccaatataa atgtcacgca cggcgtcact tttactggca
    15121 cccacggtcg ttaaatgtcc agtggcgatg atgggcagag gctgatcgcc gcgcagtttg
    15181 caggcatcgg cataatgttg ttgataataa tcagtaatag ccgccagcaa atgctgctgt
    15241 ttttcaatac cgttaagccc cgcctggctg gtaataatgt cacgcgggcg taaaaaaggt
    15301 atgggacaca gcactgcgcc tggcgtcccg tcgcgacggg gcaatatttg cggcgcatgt
    15361 ccggcgctgg cgaccacggt agtattgagg aacgccatga tatcgcgcga ttcattcagc
    15421 gtggcgaccg agtcatggtt tcctgccagt accaccagat gacagccagt ttgctgtaaa
    15481 ttgacaacaa aacggttgta taacgtgcgg gcgtaactgg gcggcgagcc ggtatcgaaa
    15541 acatcaccgg caacaataat cgcatccacc agatgggctt gtgctgtctc cagcagccag
    15601 tcaagaaaag cctgatgttc agcttcgcgg cttttactgt agaagttctg gccgagatgc
    15661 cagtctgagg tgtgaaggat gcgcataacg gttccctggc gaaaaagcat gggcgcgatt
    15721 atacccaaac agatgtgcca tttgcttttt tctgtgccac ggaaatcaat aacctgaaga
    15781 tatgtgcgac gagcttttca taaatctgtc ataaatctga cgcataatga cgtcgcatta
    15841 atgatcgcaa cctatttatt acaacagggc aaatcatggc gagacgtatt ctggtcgtag
    15901 aagatgaagc tccaattcgc gaaatggtct gcttcgtgct cgaacaaaat ggctttcagc
    15961 cggtcgaagc ggaagattat gacagtgctg tgaatcaact gaatgaaccc tggccggatt
    16021 taattctcct tgactggatg ttacctggcg gctccggtat ccagttcatc aaacacctca
    16081 agcgcgagtc gatgacccgg gatattccag tggtgatgtt gaccgccaga ggggaagaag
    16141 aagatcgcgt gcgcggcctt gaaaccggtg cggatgacta tatcaccaag ccgttttctc
    16201 cgaaggagct ggtggcgcga attaaagcgg taatgcgccg tatatcgccg atggcggtgg
    16261 aagaggtgat tgagatgcag gggctaagtc tcgatccgac atctcaccgg gtgatggcgg
    16321 gcgaagaacc gctggagatg gggccaactg aatttaaact gctgcacttt tttatgacgc
    16381 atcccgagcg cgtgtacagt cgcgagcaac tgttaaatca cgtatgggga accaacgtgt
    16441 atgtggaaga tcgtacggtc gatgtccaca ttcgtcgcct gcgtaaagca ctggagcccg
    16501 gcgggcatga ccgcatggtg cagaccgtgc gcggtacagg gtatcgtttt tcaacccgct
    16561 tttaaggcct tgctcatcgg acgtagagca gggcttatga tttcttaact ggagtatctt
    16621 acgtgctgga acggctgtcg tggaaaaggc tggtgctgga gctgctattt tgctgcctcc
    16681 cggctttcat cctgggtgca tttttgggtt acctgccctg gtttttgctg gcatcggtaa
    16741 caggactgct tatctggcat ttctggaatt tattgcgcct ttcatggtgg ctgtgggtgg
    16801 atcgtagtat gaccccgcca ccggggcgtg gtagctggga accgctacta tacggcttac
    16861 accagatgca gctgcgaaat aaaaaacgcc gccgtgaact gggcaatctg attaaacgct
    16921 ttcgtagcgg cgcggagtcg ctgcccgacg cggtggtgct gaccacggaa gagggcggta
    16981 ttttctggtg caacggtctg gcgcaacaga ttcttggctt gcgctggccg gaagataacg
    17041 ggcagaacat ccttaacttg ctgcgttacc cggagtttac gcaatatctg aaaacgcgtg
    17101 atttctctcg cccgctcaat ctggtgctca ataccgggcg gcatctggag attcgcgtca
    17161 tgccttatac ccacaaacag ttgctgatgg tggcgcgaga tgtcacgcaa atgcatcaac
    17221 tggaaggggc gcggcgtaac ttttttgcca acgtgagcca tgagttacgt acgccattga
    17281 ccgtgttaca gggttacctg gagatgatgg atgagcaacc gctggaaggc gcggtacgtg
    17341 aaaaagcgtt gcacaccatg cgtgagcaga cccagcggat ggaagggttg gtgaagcaat
    17401 tgctgacgct gtcgaaaata gaagccgcac cgacgcattt gctcaatgaa aaggttgatg
    17461 tgccgatgat gctgcgcgtc gttgagcgcg aggctcagac tctgagtcag aaaaaacaga
    17521 catttacctt tgagatagat aacggcctca aggtgtcagg caacgaagat cagctacgta
    17581 gtgcgatttc gaacctggtc tataacgccg tgaatcatac gccggaaggc acgcatatca
    17641 ccgtacgctg gcagcgagtg ccgcacggtg ccgaatttag tgttgaagat aacggaccgg
    17701 gcattgcacc ggagcatatt ccgcgcctga ccgagcgttt ttatcgcgtt gataaagcgc
    17761 gttcccggca aaccggtggt agcggattag ggttagcgat cgtgaaacat gcggtgaatc
    17821 atcacgaaag tcgcctgaat attgagagta cagtaggaaa aggtacacgt ttcagtttta
    17881 ttatcccgga acgtttaatt gccaaaaaca gcgattaatt cgcctttgtc atcttttatt
    17941 gccataagcc agccgatgct ggcttatttt ctttgcagtc gaaatacggg cgttagattt
    18001 tacaatggtt ggtgattttt tgttcgcatg attagccatg tctttttcat tgaaatagtg
    18061 ttttatactg gttggtgatt tcttatcgct atatacctct ggtttttaga tcccttcttg
    18121 ctttaaaacg ttataagcgt ttaaattgcg cttcaggtgc tgtcatactg actgcattta
    18181 cgcggtaaat cgaaaaacta ttcttcgccg cgcctggttg ggagtatttc ccgctaaaat
    18241 tgtttaaata taccgctgta tcatccccag ggattggcac aaaaatttaa cgttacaaca
    18301 ccacatccac aggcagtatg atttatgacc catcaattaa gatcgcgcga tatcatcgct
    18361 ctgggcttta tgacatttgc gttgttcgtc ggcgcaggta acattatttt ccctccaatg
    18421 gtcggcttgc aggcaggcga acacgtctgg actgcggcat tcggcttcct cattactgcc
    18481 gttggcctac cggtattaac ggtagtggcg ctggcaaaag ttggcggcgg tgttgacagt
    18541 ctcagcacgc caattggtaa agtcgctggc gtactgctgg caacagtttg ttacctggcg
    18601 gtggggccgc ttttcgctac gccgcgtaca gctaccgttt cttttgaagt gggcattgcg
    18661 ccgctgacgg gtgattccgc gctgccgctg tttatttaca gcctggtcta tttcgctatc
    18721 gttattctgg tttcgctcta tccgggcaag ctgctggata ccgtgggcaa cttccttgcg
    18781 ccgctgaaaa ttatcgcgct ggtcatcctg tctgttgccg caattatctg gccggcgggt
    18841 tctatcagca cggcgactga ggcttatcaa aacgctgcgt tttctaacgg cttcgtcaac
    18901 ggctatctga ccatggatac gctgggcgca atggtgtttg gtatcgttat tgttaacgcg
    18961 gcgcgttctc gtggcgttac cgaagcgcgt ctgctgaccc gttataccgt ctgggctggc
    19021 ctgatggcgg gtgttggtct gactctgctg tacctggcgc tgttccgtct gggttcagac
    19081 agcgcgtcgc tggtcgatca gtctgcaaac ggtgcggcga tcctgcatgc ttacgttcag
    19141 catacctttg gcggcggcgg tagcttcctg ctggcggcgt taatcttcat cgcctgcctg
    19201 gtcacggcgg ttggcctgac ctgtgcttgt gcagaattct tcgcccagta cgtaccgctc
    19261 tcttatcgta cgctggtgtt tatcctcggc ggcttctcga tggtggtgtc taacctcggc
    19321 ttgagccagc tgattcagat ctctgtaccg gtgctgaccg ccatttatcc gccgtgtatc
    19381 gcactggttg tattaagttt tacacgctca tggtggcata attcgtcccg cgtgattgct
    19441 ccgccgatgt ttatcagcct gctttttggt attctcgacg ggatcaaggc atctgcattc
    19501 agcgatatct taccgtcctg ggcgcagcgt ttaccgctgg ccgaacaagg tctggcgtgg
    19561 ttaatgccaa cagtggtgat ggtggttctg gccattatct gggatcgcgc ggcaggtcgt
    19621 caggtgacct ccagcgctca ctaaatcact gaacatttgt tttaaccacg gggctgcgat
    19681 gccccgtggt tttttattgt gttgatgggt taggaattga tggaaagtaa gaacaagcta
    19741 aagcgtgggc taagtacccg acacatacgc tttatggcac tgggttcagc aattggcacc
    19801 gggctgtttt acggttcggc agatgccatc aaaatggccg gtccaagcgt gttgttggcc
    19861 tatattatcg gtggtatcgc ggcgtatatc attatgcgtg cgctggggga aatgtcggta
    19921 cataacccgg cagccagctc tttctcgcgt tatgcgcagg aaaacctcgg cccgctggca
    19981 ggttacatta ccggctggac ctactgcttt gaaatcctta ttgtcgccat cgccgatgtg
    20041 accgcttttg gtatctatat gggtgtctgg ttcccgacgg tgccgcactg gatttgggta
    20101 ctgagtgtgg tgctgatcat ttgcgccgta aacctgatga gcgtgaaggt attcggtgag
    20161 ctggagttct ggttctcgtt ctttaaagtc gccactatca tcatcatgat tgtcgccggt
    20221 ttcggcatca tcatctgggg gattggcaac ggcgggcaac cgaccggtat tcataacctg
    20281 tggagcaacg gcggcttctt cagtaacggc tggcttggta tggtgatgtc gttgcaaatg
    20341 gtgatgtttg cttacggtgg gatcgaaatt atcgggatta ccgccggtga agcgaaagat
    20401 cctgagaaat cgattccgcg tgcgattaac tccgtgccga tgcgtattct ggtgttctac
    20461 gtcggtacgc tgttcgtcat tatgtctatc tacccgtgga atcaggttgg cactgccggt
    20521 agcccgtttg tgctgacgtt ccagcatatg ggcattacct ttgccgccag cattcttaac
    20581 tttgttgtgc tgaccgcttc gctgtcggca attaacagtg acgtatttgg tgtaggccgt
    20641 atgctccacg gtatggcaga gcagggcagc gcgccaaaaa ttttcagcaa aacctcgcgt
    20701 cgcggtattc cgtgggttac ggtgctggtg atgactaccg cgctgctgtt tgcggtgtat
    20761 ctgaactaca tcatgccgga aaacgtcttc ctggtgatcg cttcgctggc aaccttcgcc
    20821 acggtgtggg tgtggattat gatcctgctg tcgcaaatcg ccttccgtcg ccgtttgccg
    20881 ccagaagaag ttaaggcgct gaaatttaaa gtgccgggtg gggtagcaac gaccatcggc
    20941 ggtttgattt tcctgctctt tattatcggg ttgattggtt atcaccctga tacgcgtatc
    21001 tcgctgtatg ttggtttcgc gtggattgtt gtgctgttga ttggctggat gtttaagcgc
    21061 cgccacgatc gtcagctggc tgaaaaccag taatccttgc gttctgtaag ccggttcagg
    21121 aggaaatcct gatccggcta ttccgaaagt tatccgcccc cgtcctcctc ccccaaatat
    21181 ccttcagatg atgagtgatc ctgcattagg ctatggcaag gtgatcagat tttcatcaca
    21241 ggggaattat gatgttaaat gcatggcatc tgccggtgcc cccatttgtt aaacaaagca
    21301 aagatcaact gctcattaca ctgtggctga cgggcgaaga cccaccgcag cgcattatgc
    21361 tgcgtacaga acacgataac gaagaaatgt cagtaccgat gcataagcag cgcagtcagc
    21421 cgcagcctgg cgtcaccgca tggcgggcgg cgatcgagct ctcctgcgga caacctcggc
    21481 ggcgttatag tttcaaattg ctgtggcacg atcgccagcg ttggtttaca ccgcaaggct
    21541 tcagccgaat gccgccggca cgacaggagc agtttgccat cgatgtaccg gatatcggcc
    21601 cacaatgggc tgcggatcag attttttatc agatcttccc tgatcgtttc gcccgtaatc
    21661 ttcctcgtga agctgaacag gatcatgtct attaccatca cgcagccgga caagagatca
    21721 tattgcgtga ctgggatgaa ccggtcacgg cgcaggctgg cggatcaacg ttctatggcg
    21781 gcgatctgga cgggataagt gaaaaactgc cgtatctgaa aaaacttggc gtgacggcgc
    21841 tgtatctcaa tccggtgttt aaagctccca gcgtgcataa atacgatacc gaggattatc
    21901 gccatgtcga tccgcagttt ggcggtgatg gggcgttgct gcgtttgcga cacaatacgc
    21961 agcagctggg aatacgactg gtgctggacg gcgtgtttaa ccacagtggc gattcccatg
    22021 cctggtttga caggcacaat cgtggcacgg ggggagcttg tcacaacccc gaatcaccct
    22081 ggcgtgactg gtactcgttt agtgatgatg gcacggcgct cgactggctg ggctatgcca
    22141 gcttgccgaa gctggattat cagtcggaaa gtctggtgaa tgaaatttat cgcggggaag
    22201 acagtattgt ccgccactgg ctgaaagcgc cgtggaatat ggatggctgg cggctggatg
    22261 tggtgcatat gctgggggaa gcgggtgggg cgcgcaataa tttgcagcac gttgccggga
    22321 tcaccgaagc ggcgaaagaa acccagccgg atgcgtatat tgtcggcgaa cattttggcg
    22381 atgcacggca atggttacag gccgatgtgg aagatgccgc catgaactat cgcggcttca
    22441 cattcccgtt gtggggattt cttgccaaca ccgatatctc ttacgatccg cagcaaattg
    22501 acgcccaaac ctgtatggcc tggatggata attaccgcgc agggctttct catcaacaac
    22561 aattacgtat gtttaatcag ctcgacagcc acgatactgc gcgatttaaa acgctgctcg
    22621 gtcgggatgt tgcgcgcctg cccctggcgg tggtctggct gttcacctgg cctggtgtac
    22681 cgtgcattta ttacggtgat gaagtgggac tggatggcaa aaacgatccg ttttgccgta
    22741 agccgttccc ctggcaggtg gaaaagcagg attcggcgtt attcgcgctg taccagcgaa
    22801 tgattgcgct gcgtaagaaa agtcaggcgt tgcgtcgtgg cggctgtcag gtgctgtatg
    22861 cggaagataa cgtggtggta tttgtccgcg tgctgaatca gcagcgtgta ctggtggcaa
    22921 tcaaccgtgg cgaagcctgt gaagtggtgc tacccgcgtc gccgttgctt aatgtcgcgc
    22981 aatggcaacg caaagaaggt cacgggcaac tgacggacgg gattctggct ttgcctgcca
    23041 tttcggctac ggtgtggatg aattaacgtc ttataacgcc ttgcgtgacg cctgctccat
    23101 catccgcgga taaaactgcc agaagcgggt ttcgagagca tcgtaatggg cgtctaaatc
    23161 gtaccaggag tcacgcaggg catccagacg tgggcgacgg cttgccatgc cgtttaacac
    23221 gctctggatg aaatccatat cgcgatagcg caccaaccac tgctctgacc ataagtagtt
    23281 gttcagattg ataaagcgtg gcggtgagtc tggaagaata gtcatcactt gctcccgggc
    23341 ataacaggta aattcctgta gcggaaaatc gggcgacagc tgcgaccagt ggcgggacag
    23401 aaagtgatcc cacatcacat ccagcgtaat aggcgcgaca cggcgcgttt cattacgaaa
    23461 ccactcccgc gcttcgcgga cttctggcag attgtcggtt aatacgtcga ttcgccgatg
    23521 catatgaatg ccagccacga cgtcgggcgg aaaactttct tcaggatttc cgcgtacgaa
    23581 atcagccagt aaattgccgg aaagcgagct ttccgcgaga tgggctaaat gcaggtgagc
    23641 taaaaaattc atcgattata ttctatccaa aggggggtaa aggttgcagg gagagcgccc
    23701 cggcactaga ctacccgcct cttattttag tctgagtcag tgtcatgcgc gttaccgatt
    23761 tctcttttga attgcccgaa tccctgattg cccactatcc catgcctgaa cgcagtagct
    23821 gtcgtttact gtcgctggac gggccgacgg gcgcgctgac gcacggtact ttcaccgatt
    23881 tactcgataa gctcaacccc ggcgatcttc tggtttttaa taatacccgc gtgatcccgg
    23941 cgcgtctgtt tgggcgtaaa gccagcggcg gcaagattga agtgctggtt gagcggatgc
    24001 tcgacgacaa acgcattctt gcgcatattc gcgcctcgaa agcgccaaaa cctggcgcag
    24061 aactgctgct gggcgatgac gaaagtatta acgcaacaat gaccgcgcgc cacggcgcac
    24121 tgtttgaagt cgaatttaat gatcaacgtt cggtgctgga tattctcaac agcatcggcc
    24181 atatgccgct gccgccgtac atcgaccgtc cggacgaaga tgcagaccgc gaactttatc
    24241 aaacggttta tagcgaaaaa cccggcgcgg tagcagcacc gaccgccgga ctgcactttg
    24301 acgaaccttt gctggaaaaa ttacgcgcca aaggcgtgga gatggcgttt gtgacgttgc
    24361 acgttggtgc gggcaccttc cagccggtgc gcgtcgacac cattgaagat cacatcatgc
    24421 actcggaata cgctgaagtg ccgcaggatg tggtagacgc ggtactggcg gcgaaagcgc
    24481 gcggtaaccg ggtgattgcg gttggcacca cctcagtacg ttcgctggaa agtgcggctc
    24541 aggcagcgaa aaacgatctc attgaaccgt tctttgacga tacccaaatc tttatctatc
    24601 cgggcttcca gtacaaagtg gtcgatgcgc tggtgacgaa cttccacctt ccggaatcga
    24661 cgctgattat gctggtttcg gcatttgctg gttatcaaca caccatgaat gcctataaag
    24721 cggcggtaga agagaaatat cgctttttta gttacggtga tgcgatgttt atcacgtaca
    24781 atccgcaggc aattaatgag cgcgttgggg agtaattccg cggcgctggt ttaaacgttg
    24841 gactgttttt ctgacgtagt ggagaaaaaa tgaaatttga actggacacc accgacggtc
    24901 gcgcacgccg tggccgcctg gtctttgatc gtggcgtagt ggaaacgcct tgttttatgc
    24961 ctgttggcac ttacggcacc gtaaaaggga tgacgccgga agaagttgaa gccactggcg
    25021 cgcaaattat cctcggcaac accttccacc tgtggctgcg cccgggccag gaaatcatga
    25081 aactgcacgg cgatctgcac gattttatgc agtggaaagg accaatcctt accgactccg
    25141 gcggcttcca ggttttcagc cttggtgata ttcgtaaaat caccgaacag ggtgttcact
    25201 tccgtaaccc gatcaacggc gacccgattt tcctcgaccc ggaaaagtcg atggagattc
    25261 agtacgatct tggttcggat atcgtcatga tctttgatga gtgtacgccg tatcctgctg
    25321 actgggatta tgcaaaacgc tccatggaga tgtctctgcg ttgggcgaag cgtagccgtg
    25381 agcgttttga cagtctcgga aacaaaaatg cgctgtttgg tatcattcag ggcagtgttt
    25441 acgaagattt acgtgatatt tctgttaaag gtctggtaga tatcggtttt gatggctacg
    25501 ctgtcggcgg tctggctgtg ggtgagccga aagcagatat gcaccgcatt ctggagcatg
    25561 tatgcccgca aattccggca gacaaaccgc gttacctgat gggcgttggt aaaccagaag
    25621 acctggttga gggcgtacgt cgcggtatcg atatgtttga ctgcgtaatg ccaacccgca
    25681 acgcccgaaa tggtcatttg ttcgtgaccg atggcgtggt gaaaatccgc aatgcgaagt
    25741 ataagagcga tactggccca ctcgatcctg agtgtgattg ctacacctgt cgcaattatt
    25801 cacgcgctta cttgcatcat ctcgaccgtt gcaacgaaat attaggcgcg cgactcaaca
    25861 ccattcataa ccttcgttac taccagcgtt tgatggcggg tttacgcaag gctattgaag
    25921 agggtaaatt agagagcttc gtaactgatt tttaccagcg tcaggggcga gaagtaccac
    25981 ctttgaacgt tgattaatat taataatgag ggaaatttaa tgagcttttt tatttctgat
    26041 gcggtagcgg caacgggtgc accggcgcaa ggtagcccga tgtctttgat tttgatgctg
    26101 gtggtattcg gtctgatttt ctatttcatg atcctgcgtc cacagcagaa gcgcaccaaa
    26161 gaacacaaaa agctgatgga ctccatcgct aaaggtgatg aagtgctgac gaacggtggc
    26221 ctggtcggtc gcgtaaccaa agtagcggaa aacggctaca ttgctatcgc actgaatgac
    26281 accactgaag tagttattaa acgtgacttc gtagctgccg ttctgccgaa aggcaccatg
    26341 aaggcgctgt aattaaaatt tttccctaag ggaattgccg tgttaaaccg ttatcctttg
    26401 tggaagtacg tcatgctgat cgtggtgatt gtcatcggtc tgctgtatgc gcttcccaac
    26461 ctgtttggtg aggatccggc tgttcagatc actggtgcgc gcggagtcgc cgccagtgag
    26521 caaacgctga tccaggtcca gaaaacgtta caagaagaaa aaataactgc taagtctgtg
    26581 gcactggaag agggcgctat tcttgcgcgc ttcgactcca ctgacaccca gctgcgcgct
    26641 cgtgaagcat taatgggcgt tatgggtgac aaatacgtcg tggcgcttaa tcttgccccg
    26701 gcaacgccgc gctggctggc agctattcat gctgagccga tgaagctcgg ccttgacctg
    26761 cgtggcggcg ttcacttcct gatggaagtg gatatggaca ccgcgcttgg caaactccag
    26821 gaacaaaata tcgatagcct gcgcagcgac ctgcgtgaaa agggcatccc gtataccacc
    26881 gttcgtaaag aaaacaacta cggcctgagc atcactttcc gcgatgctaa agctcgtgat
    26941 gaagccattg cgtatctgag caagcgccat ccggacctgg tgattagcag ccagggcagc
    27001 aaccagctgc gtgcggtaat gagcgatgct cgtctgagtg aagcgcgtga atatgcggtg
    27061 cagcagaaca tcaatatcct gcgtaaccgt gtaaaccaac ttggcgtggc ggagccggtg
    27121 gttcagcgtc agggtgctga ccgtatcgtt gttgaactgc caggtattca ggacactgcg
    27181 cgtgcgaaag agattctggg tgcgacggca acgctggaat tccgtctggt aaacaccaac
    27241 gttgatcagg ccgctgcggc atccggtcgc gtaccgggcg actctgaagt gaaacagacc
    27301 cgcgaaggtc agccggttgt gctgtacaaa cgcgtaattc tgaccggtga ccatatcacc
    27361 gactccactt ccagccagga cgaatacaac cagccgcagg ttaacatctc tctcgatagc
    27421 gcgggtggta acatcatgtc taacttcacc aaggacaaca tcggcaaacc gatggcaacc
    27481 ctgtttgtgg agtacaaaga cagcggtaag aaagatgcaa acggtcgtgc ggttctggtg
    27541 aaacaggaag aggtgattaa tatcgccaac atccagtctc gtctgggtaa cagcttccgt
    27601 attactggca tcaataaccc aagcgaagcg cgtcagcttt cactgctgct gcgtgccggt
    27661 gcgttgatcg cgccgattca gattgttgaa gaacgtacca ttggcccaac cctgggtatg
    27721 cagaacattg agcaggggct ggaggcttgc cttgccggtc tgctggtttc tattctgttc
    27781 atgatcatct tctataagaa gtttggtctg attgcgacca gtgctctgat tgccaacttg
    27841 atcttgattg tcggtattat gtcgctgttg ccaggcgcaa cgctgagtat gccaggtatc
    27901 gcgggtatcg tcttaaccct tgcggtagcg gtcgatgcga acgtactgat caacgaacgt
    27961 attaaagaag agttgagcaa cggacgtact gttcaacagg caattgatga aggttatcgt
    28021 ggcgcattca gttccatctt cgatgcgaac atcaccacgc tgattaaagt catcatcctg
    28081 tacgcagtgg gtaccggggc aattaaaggg ttcgcgatta ctaccggtat cggtgtggcg
    28141 acgtcgatgt ttaccgcgat tgtcggtacg cgtgccatcg taaacctgct atatggcggc
    28201 aagcgcgtca agaagctgtc aatctgagga gtgcgatgtg gcacaggaat atactgttga
    28261 acaactaaac cacggccgta aagtctatga ctttatgcgc tgggactact gggctttcgg
    28321 catctctggt ctgctgttaa tcgccgctat cgttattatg ggcgtgcgcg gctttaactg
    28381 ggggctggat ttcaccggtg gtacggttat tgaaattacg ctcgaaaaac cggctgaaat
    28441 tgacgtaatg cgtgatgcat tgcaaaaagc cggttttgaa gagccgatgc tgcaaaactt
    28501 tggtagcagc cacgacatca tggtccgtat gccgcctgct gaaggcgaaa ccggtggtca
    28561 ggtgctgggc agccaggttc tgaaggtgat taacgaatcc accaatcaga atgcggcagt
    28621 gaagcgtatt gagttcgtcg gtccgagcgt gggggcagac cttgcgcaaa ccggtgcgat
    28681 ggcgttgatg gcggcgctgc tgtctatcct cgtgtacgtt ggtttccgct ttgagtggcg
    28741 actggcggca ggggttgtta ttgcgctggc gcacgacgtt atcattacgc tgggtatttt
    28801 gtcgttattc catatcgaga ttgacctgac cattgtggca tcgttgatgt cggttatcgg
    28861 ttactcgctt aacgacagta tcgtggtatc ggaccgtatt cgtgaaaact tccgcaagat
    28921 ccgtcgcggt acgccttacg aaatctttaa cgtgtccttg acccagacgc tgcaccgtac
    28981 cttgatcaca tccggtacta ccttgatggt tatcctgatg ctgtacctct tcggtggtcc
    29041 ggtactggaa ggcttctcgc tgaccatgct tatcggtgtt tccatcggta ctgcatcttc
    29101 catctatgtg gcatctgcgc tggctctgaa actgggtatg aagcgcgaac acatgttgca
    29161 gcagaaagtg gaaaaagaag gggcggatca gccgtcaatt ctgccttaat caagtttcag
    29221 ctgatgttga aaatcccggt cagaagatcg ggattttttt tgatgtaagg tgattgcgaa
    29281 aatccccgca tcttgggaaa ctgcgcgtaa ccctacattt catccaggta actttttcat
    29341 ggctatcatc ccaaaaaact atgcgcggtt agaaagcggc tatcgcgaaa aagcattaaa
    29401 aatctatccg tgggtctgcg gtcgctgttc ccgcgagttt gtttattcca acctgcgtga
    29461 acttaccgtt caccacattg atcacgacca tactaataac ccggaagatg gcagtaactg
    29521 ggaattactt tgtctctatt gccacgatca tgagcattcg aaatacaccg aagcggatca
    29581 gtatggtacg accgttatcg caggggaaga tgcgcagaaa gatgtcggtg aagcgaagta
    29641 caacccattc gctgatttga aagcgatgat gaacaagaag aagtgataaa aaatgccgga
    29701 tatgaaatcc ggcattctca caattagaag ttgtaaccta ctaccagata accaccccag
    29761 ccggtagagc gaacgttgaa gttgccgttg ccgaagttca gttctgcatc gtcattccac
    29821 tgaccaccgt tatgccagta acgggcaacg acagagtagt gccagtgatc gtaattcagc
    29881 gccagaatat ggctggacgc aatagagtta ttggtacgtg ttttaatacc gttgttcgca
    29941 taaccgctgt catcgcccag gtcggaaccc cagtcgaagt tggtgaagcc gatgtagctc
    30001 agctggccgc cccacagatc ggtaatcggc acaaagtact tcactttgaa acggtaaccg
    30061 tcccactcgt tttcgttcgc tgcgccgtag ttctgccact ggtatttcgc atagacgttc
    30121 atggacaggc tcatcggcag accagtatcg atgtcggtac ccagacccat gtaccaggtg
    30181 ctctggcggc catctttatt acgacccatg tcgtaaatgt agttgttcgc gaagtaccac
    30241 tctttgaacg gaccgaagct aaggtcagta ttggtcagct tgtcaatgga gaaacgcggt
    30301 tcgatttcca taaacagcgg agaaccgtgg ttccagatac cttttgcatc ggagttaccg
    30361 ccgaagaata ccggcgcatc cgcataacca tagaagtcga accagtcttt tttagcgaat
    30421 gcttcgtact caaggtaggt atcgttgcgg agctgcggtc cgaaacgggt gtgatagctt
    30481 ccgacaacgt taacgctctg gtgccaccag tcggaaagat actgcggttt gtcgttttca
    30541 gctgcgttga cagtaaaaga cgaagagagc gccagtaccg caccggctgc cagtaatgtt
    30601 tttttcatat gtatgccact gtttgaaaat cccttgcggg agtgaaaaag gcgcaaattg
    30661 cgtttctaaa tatttcgtat acagaggagc ctattataaa aatcattgct cacaaaaata
    30721 tgtttcgttt cacagttcta tcatttacgt aatcgattgc gttcacgttt acacacattc
    30781 gggcggggat tgtactgact ttcacccttg ttgcaaactt ttactatcaa tccaggtgat
    30841 gtaagcggag taacaaaatg acggagaaag atgttgcggg acgcacaata tcgccccgca
    30901 atacgtttac tgcataacag gttgaatgtc gtggatacgt acaaagccta actgatccac
    30961 agaaataccc tttaactgta acggaatatc aacatcgctg ggggcgagga cgctggcagg
    31021 ggcgttaatc aattgattct gcacattgat ttcctgaaag ttgtctattg ttccctgaat
    31081 ttgcccatat tcaacggttc cgctgaatgc gggtaaaggg tcattggatt cgccctgaat
    31141 acgtagcgtc agggtagtgc catctgtgtc aggcgtaata ttcaccagcg acatacgtaa
    31201 agtaccgatc tggctttcca gtcttgctgg tgtttttgct ccaggcagaa ggtaaacccc
    31261 actgctggat tttgcattca gcttgttttg ctgagtgatt ttgaccgttt ctttattgag
    31321 ttgcgtcatc tcctggttca gcgtgctgac gctgtgtttc atctgtcgca cttcactttg
    31381 ctgcacacag gcgctaaggc tgaacaggct tcccagcagg agcagtcgaa aaacgttagt
    31441 attcataggt cgtccctttc tcgtcgcaat tagcctaatg gtagagggta aaagcagtga
    31501 aagcatcgat cctttatctc aaaagcgttg cgcctttgtt gtatcgtcag ttcagggtaa
    31561 aatagatttc tgttaaccac ctggtcagga cgccgtatgc attgcccatt ctgtttcgcc
    31621 gtggacacta aggtaattga ctctcgtctc gtgggcgagg gttcatccgt acgccgccgt
    31681 cggcagtgtc tggtgtgtaa tgaacgtttc accacctttg aagtggcgga gctggttatg
    31741 ccgcgtgttg taaaaagcaa cgacgtgcgt gaaccgttta atgaagagaa attacgtagc
    31801 ggaatgctgc gggcgctgga aaaacgtccg gtgagttccg atgacgtcga aatggcaatc
    31861 aatcatataa aatcgcagct gcgcgccacc ggtgagcgcg aagtgccgag caagatgatt
    31921 ggcaatctgg tgatggagca attgaaaaag ctcgataaag tcgcctatat ccgttttgcc
    31981 tctgtctacc gcagtttcga agatatcaaa gaatttggcg aagagatcgc gcgcctggag
    32041 gactaagccg tgcaggacga gtattacatg gcgcgggcgc taaagctggc gcaacgagga
    32101 cgttttacca cgcatcccaa cccgaatgtc gggtgcgtca ttgtcaaaga tggcgaaatt
    32161 gtcggtgaag gttaccatca acgagcgggt gaaccgcatg ccgaggtaca cgcgttgcgc
    32221 atggccggcg aaaaagccaa aggcgcaacg gcgtatgtca cactcgaacc ctgtagccat
    32281 catggtcgta cgccaccgtg ttgtgatgca ttaattgctg cgggcgtggc gcgcgtggtt
    32341 gctgcaatgc aagaccccaa tccgcaggtt gctggacgtg gactttaccg tctgcaacag
    32401 gctggcattg acgtcagcca cgggctgatg atgagtgaag ctgagcaatt gaataaaggc
    32461 tttctcaagc ggatgcgcac cggctttcct tatattcagt taaaacttgg cgcatcgctt
    32521 gatggtcgca cggcgatggc gagtggcgaa agccagtgga tcacttcgcc tcaggcgcga
    32581 cgcgacgtac aacgactgcg cgcgcaaagt catgccattt taaccagcag cgccacggtg
    32641 ctggcggatg atccagcctt aacagtgcgt tggtctgaac tggatgaaca aactcagggg
    32701 ctctacccgc aacaaaatct ccgtcagcca gtacgtattg tgattgatag ccaaaatcgc
    32761 gtgacgccgg aacatcgcat tgtgcagcag cccggcgaaa cctggttcgc gcgtacgcag
    32821 gaagattctc gtgagtggcc ggaaacggtg cgtaccttgc tgattccaga gcataaaggt
    32881 cacctggatc tggttgtact aatgatgcaa ctgggtaaac agcagattaa cagcatctgg
    32941 gtggaagcgg ggccaacgct cgctggcgca ttgctacagg cgggtttagt cgatgagctg
    33001 attgtctata tcgcacctaa actattaggc aacgacgccc gtggattatg cacgctgcca
    33061 gggcttgaga aattagccga cgctccccaa tttaaattca aagagatacg tcatgtaggc
    33121 ccggatgttt gcctgcattt agtgggtgca tgatctcccg gctcgaaagg gaagcagcgc
    33181 acgaaatatt atgctaaaat ccgcccccct gcggggccat actcgaaccc gaaggaagaa
    33241 aatgaacatt attgaagcta acgttgctac cccggacgct cgcgtcgcca tcaccatcgc
    33301 gcgtttcaac aactttatca atgacagcct gctggaaggt gcaattgacg cactgaaacg
    33361 tatcggtcag gtaaaagatg aaaacattac cgttgtttgg gtgcctggtg cctatgagct
    33421 gccgctggcg gcgggtgcac tggctaaaac cggtaaatac gacgcggtga ttgcgctggg
    33481 tacggttatt cgtggtggca ctgcccactt tgaatatgtc gctggtggtg caagcaacgg
    33541 cctggcgcat gttgcccagg acagcgaaat tccggttgct tttggtgttc tgaccactga
    33601 aagcattgaa caagcgatcg aacgtgctgg caccaaagct ggcaacaaag gtgcagaagc
    33661 tgcactgacc gcgcttgaaa tgattaatgt attgaaagcc atcaaggcct gaaattagta
    33721 aggggaaatc cgtgaaacct gctgctcgtc gccgcgctcg tgagtgtgcc gtccaggcgc
    33781 tctactcctg gcagttgtcc cagaacgaca tcgctgatgt tgaataccag ttcctggctg
    33841 aacaggatgt aaaagacgtt gacgtcctgt acttccgtga gctgctggcc ggggtggcga
    33901 ctaacaccgc atacctcgac ggactgatga agccgtacct gtcccgtctg ctggaagaac
    33961 tgggccaggt agaaaaagca gtactgcgca ttgcgctgta cgagctgtct aaacgtagcg
    34021 atgtgccata caaagtggcc attaacgaag cgatcgaact ggcgaaatcg ttcggcgcag
    34081 aagacagcca taagttcgtc aacggcgtac tcgataaagc agcacctgtg attcgcccta
    34141 acaaaaagta atatccaagc cggtagattc acggaagacc gttccatgat cgccggcctt
    34201 ttctttttta tctgctgagg cgtaacgtat ggcatgtggc gagttctccc tgattgcccg
    34261 ttattttgac cgtgtaagaa gttctcgtct tgatgtcgaa ctgggcatcg gcgacgattg
    34321 cgctcttctc aatatccccg agaaacagac cctggcgatc agcactgata cgctggtggc
    34381 gggcaaccac ttcctccctg atatcgatcc tgctgatctg gcttataaag cactggcggt
    34441 gaacctaagc gatctggcag cgatgggggc cgatccggcc tggctgacgc tggcattaac
    34501 cttaccggac gtagacgaag cgtggcttga gtccttcagc gacagcttgt ttgatcttct
    34561 caattattac gatatgcaac ttattggcgg cgataccacg cgtgggccat tatcaatgac
    34621 gttgggtatc cacggctttg ttccgatggg acgagcctta acgcgctctg gggcgaaacc
    34681 gggtgactgg atctatgtga ccggtacacc gggcgatagc gctgccgggc tggcgatttt
    34741 gcaaaaccgt ttgcaggttg ccgatgctaa agatgcggac tacttgatca aacgtcatct
    34801 ccgtccgtcg ccacgtattt tacaggggca ggcactgcgc gatctggcaa attcagctat
    34861 cgatctctct gacggtctga tttccgatct cgggcatatc gtgaaagcca gcgactgcgg
    34921 cgcacgtatt gacctggcat tgctgccgtt ttctgatgcg ctttctcgcc atgttgaacc
    34981 ggaacaggcg ctgcgctggg cgctctctgg cggcgaagat tacgagttgt gttttacggt
    35041 gccggaactg aaccgtggcg cgctggatgt ggctctcgga cacctgggcg taccgtttac
    35101 ctgtatcggg caaatgaccg ccgatatcga agggctttgt tttattcgtg acggcgaacc
    35161 tgtcacgtta gactggaaag gatatgacca ttttgccacg ccataaagat gtcgcgaaaa
    35221 gtcgcctgaa aatgagtaat ccgtggcatc tgcttgctgt cggattcgga agtggattaa
    35281 gcccgatcgt tcctggcacg atggggtcgc tggcggcgat tcccttctgg tatctgatga
    35341 cttttttgcc ctggcagctc tattcgctgg tggtgatgct ggggatctgt atcggcgtct
    35401 atctttgtca tcaaacggcg aaagacatgg gcgtgcacga tcacggcagt attgtctggg
    35461 acgaatttat tggtatgtgg attacgctca tggcgctgcc gaccaatgac tggcagtggg
    35521 ttaccgccgg atttgtgatt ttccgtattc tggatatgtg gaagccgtgg ccgatccgct
    35581 ggtttgatcg caatgtacat ggcggcatgg ggatcatgat tgacgatatt gtcgccgggg
    35641 tgatctccgc agtcatcctg tactttattg gacatcactg gccgctgggt attctgtcgt
    35701 agttgtgcac cgatgcctga tgtgacgctt gtcacgtctc atcaggcctg tatacctatt
    35761 taaatcctac gaccggatgc ggtttatacg gcgtttccag ctcggcgatt tgttccggct
    35821 tcaaggtgat atccaccgcg tttagcagtt catcaagctg ttcctcccga gaagttccga
    35881 taatcggtgc ggcaatgccc ggtttactca acaaccaggc cagcgcaact tgtgctcgcg
    35941 ttgcaccaag ttcttcactg acgcccgtta agcgttctgc aatttgtgcg tcattttcat
    36001 cgctttcttg gtagagattt ttccccactt catccgacac cagccgtgcg gtggtttctc
    36061 cccaaggacg cgtcagacgg ccccgcgcca gcgggctcca tggaatcacc gccacgcctt
    36121 cctgataaca cagcggcagc atctcgcgct cttcttcacg ataaatcaga ttgtagtgat
    36181 cctgcatact gacaaactgc gcccagccgt gctgtttttg tagttccagt gcttgagcaa
    36241 actgcgaagc gtgcatggat gacgcgccga tataacgcgc tttcccgact tttaccacgt
    36301 cgttcagggc ttccagcgtc tcttcgatcg gcgtgttgta atcccagcga tgaatttgca
    36361 ggatatcgac ataatccatg ccgagacggc gcaggctgtc gtcgatagag cgcaaaattt
    36421 gtgcacggga taatccttca ggtaaatcac caacgcgatg gaacactttg gtcgcaacga
    36481 ccacgtcttc acgacgggcg aaatcccgca gtgcgcgacc gacgatctct tcgctgctgc
    36541 catcggaata gctattggcg gtatcaaaga aatttatgcc gccttccagc gcacgtttaa
    36601 tgatgggacg gctgctctct tccggcagtg tccatgcgtg attaccgcga tctggctcgc
    36661 caaaggtcat acatccgagg caaagtcggg aaacgcgaag gtcggttttt cctaaggggt
    36721 tgtattgcat gctgccactc ctgccatgaa taatttctta agcatagcag gagtggagta
    36781 gggattatgc cagccaggct ttgattttgg cttccatacc ggtggcatcg aggccgagtt
    36841 cggcgcgcat ttcttcctga gttccttgtg gaataaagaa gtccggcagg ccaatgttca
    36901 gcacgggtac tggtttacga tgggccatca acacttcgtt cacgccgctg cctgcaccgc
    36961 ccataatggc gttttcttct acggtaacca gcgcttcatg gctggcggcc atttccagaa
    37021 ttaacgcttc atcaagcggt ttcacgaaac gcatatcgac cagcgtagcg ttcagcgatt
    37081 cagcgacttt cgccgcttct ggcatcagcg taccaaagtt aaggatcgcc agtttctcac
    37141 cacgacgctt cacaatgcct ttgccaattg gcagtttttc cagtggcgtc agttccacgc
    37201 caactgcgtt gccgcgcggg tagcgcaccg cggacgggcc atcgttatag tgatagccgg
    37261 tatagagcat ctggcgacat tcgttttcat cgctcggggt cataatgacc atttccggta
    37321 tgcagcgcag gtaagagaga tcaaaagcgc cctggtgggt ttgaccgtca gcgccaacaa
    37381 tgcccgcacg gtcgatggcg aacaggaccg gaagcttttg aatcgccacg tcatgcagca
    37441 cctgatcata ggcgcgttgc aggaaggtgg agtagatcgc gacaatgggt ttgtagcctc
    37501 caatcgccag acccgcagca aaggttactg cgtgttgctc ggcaattgcc acgtcaaagt
    37561 agcgatccgg gaatttacgt gaaaactcga ccatgccgga accttcacgc atcgccggag
    37621 taatcgccat cagcttgtta tctttcgctg cggtttcgca caaccagtcg ccaaagattt
    37681 ttgaatagct tggtaaaccg ccgctacttt tcggcagaca accgctggag ggatcaaatt
    37741 taggcacggc gtggaaggtg attgggtctt tttctgccgg ttcataacca cgaccttttt
    37801 tggtcatgat atgcaggaac tgcgggcctt tcaggtcgcg catgttctta agcgtggtga
    37861 taagccccag cacatcgtga ccgtccaccg ggccgatgta gttaaagccc agctcttcaa
    37921 acaacgtgcc aggcactacc atgcctttaa tatgttcttc ggtacgtttg agcagctctt
    37981 taatgggtgg cacgccagag aaaacttttt ttccgccttc gcgcagtgaa gagtaaagct
    38041 taccggaaag cagctgcgcc agatggttgt tgagcgcgcc aacattttcg gaaatcgaca
    38101 tttcattgtc gttgagaacc accagcatat caggacggat atcgcccgcg tgattcatcg
    38161 cttcaaacgc catgccagca gtaatcgcgc catcgccaat gacacagacg gtgcggcgat
    38221 ttttgccttc tttctcggca gcaaccgcaa taccaattcc ggcactgatg gaggttgatg
    38281 aatgcccgac gcttaatacg tcatattcgc tttcgccgcg ccacgggaac gggtgcaggc
    38341 cacctttctg acggatggtg ccgattttgt cgcggcgccc ggtcaaaatt ttatgcggat
    38401 aagcctgatg ccccacatcc cagattaact ggtcaaacgg ggtattgtag acatagtgca
    38461 gcgccacggt cagttcgacc gtgcccagcc cggaggcgaa gtgcccgctg gaacggctca
    38521 cgctgtcgag taaatagcgg cgcagttcgt cgcagagttt cggtaaactc tctttcggca
    38581 acagtcgtaa ctcctgggtg gagtcgacca gtgccagggt cgggtatttg gcaatatcaa
    38641 aactcatcag gggcctatta atacttattg tttatttatt acgctggatg atgtagtccg
    38701 ctagcgcttc cagtgccgag gtatcgaatg actgttcagt cagttgtttc agcgactgac
    38761 gggcatcgtc gatcagatcc cgggctttct tccgggcttg ctcaagaccc agaagtgcag
    38821 ggtaggtact tttaccaagt tgctggtcgg caccctggcg ttttcccaac gttgcagtat
    38881 ctcccaccac atccaggatg tcatcctgaa cctggaaggc aaggccgatg ctctctgcgt
    38941 acttgtcgag tactggcaga gcacgacgtc ctttatctcc ggcgcttaat gcaccaaggc
    39001 gaacggcggc gcgaatcaat gcgccggttt tatgacgatg aatacgctca agcgcgtcca
    39061 gaggtacgtg tttgccttca gcgtctaaat ctaatgcctg accaccgcac attccggcaa
    39121 taccgctggc gctcgccaac tcagaaatca tcgaaattct gtcgcgatct gacacttccg
    39181 gcatatcggc atcgcttaga atcgagaacg ccagcgtttg taaagcgtca ccagcgagaa
    39241 tcgcgtttgc ttcgccaaac ttcacatggc aggtcggcaa accgcgacgc agatcgtcat
    39301 catccatcgc cggtaaatca tcatgaatta atgagtaagc gtggatacac tctacggcgg
    39361 cagcgggtgc gtccagtgtg tttgtgctaa cgccgaacat atgaccagtg gcataaacca
    39421 ggaaaggtcg caggcgctta ccacctaata atgcgccata ctgcatggtt tcgaccacgg
    39481 gagtgttctg aaagggcagt ggggcgataa aacggctcag cgcctggttg gcctgcttaa
    39541 cgcaggcttc gagttgctgc ggaaagtcca ttactcattg tccggtgtaa aaggggttag
    39601 agaggcgtct tcattgtcag acagcagaat ttgtacgcgc tgttcggctt gttgtaattt
    39661 agcctgcccc tgacgtgcca gctgcacgcc gcgttcgaac tcgttcagcg cctcttccag
    39721 cggcaggtcg ccactttcca gacgggttac aatctgttcc agctcgctca gcgccttttc
    39781 aaagctggcg ggagcctcat ttttcttcgg cataatgaat gtctgactct caatattttt
    39841 cgccccgtca tggtaacgga ctcagggcaa atagcaaata acgcgcaatg gtaaggtgat
    39901 gtgcgcagca aagcgatgtt agtggtatac ttccgcgcct ggatgcagcc gcaggtgtgg
    39961 gctgctgtat ttttccctat acaagtcgct taaggcttgc caacgaacca ttgccgccat
    40021 gaagtttatc attaaattgt tcccggaaat caccatcaaa agccaatctg tgcgcttgcg
    40081 ctttataaaa atccttaccg ggaacattcg taacgtttta aagcactatg atgagacgct
    40141 cgctgtcgtc cgccactggg ataacatcga agttcgcgca aaagatgaaa accagcgtct
    40201 ggctattcgc gacgccctga cccgtattcc gggtatccac catattctcg aagtcgaaga
    40261 cgtgccgttt accgacatgc acgatatttt cgagaaagcg ttggttcaat atcgcgatca
    40321 gctggaaggc aaaaccttct gcgtacgcgt gaagcgccgc ggcaaacatg attttagctc
    40381 gattgatgtg gaacgttacg tcggcggcgg tttaaatcag catattgaat ccgcgcgcgt
    40441 gaagctgacc aatccggaag tgactgtcca tctggaagtg gaagacgatc gtctgctgct
    40501 gattaaaggc cgctacgaag gtattggcgg tttcccgatc ggcacccagg aagatgtgtt
    40561 gtcgctcatt tccggtggtt tcgactccgg tgtttccagt tatatgttga tgcgtcgcgg
    40621 ctgccgcgtg cattactgct tctttaatct cggcggcgcg gcgcatgaaa ttggtgttcg
    40681 tcaggtggcg cattatctgt ggaaccgttt tggtagctcc caccgcgtgc gttttgtcgc
    40741 cattaacttt gagccggtcg tcggtgaaat tctcgagaaa atcgacgacg gtcagatggg
    40801 cgttatcctc aaacgtatga tggtgcgtgc cgcgtccaaa gtggcagaac gttacggcgt
    40861 acaggcgctg gttaccggtg aagcgctcgg ccaggtgtcc agccagacgc tgaccaacct
    40921 gcgcctgatt gataacgtct ccgatacgct gatcctgcgt ccgctgatct cttacgacaa
    40981 agagcacatc atcaacctgg ctcgccagat tggcaccgaa gactttgccc gcacgatgcc
    41041 ggaatattgc ggcgtgattt ccaaaagccc gacggtgaaa gcggttaaat cgaagattga
    41101 agcggaagaa gagaagttcg acttcagcat tctcgataaa gtggtggagg aagcgaataa
    41161 cgttgatatc cgcgaaatcg cccagcagac cgagcaggaa gtggtggaag tggaaaccgt
    41221 caatggcttc ggcccgaacg acgtgatcct cgatatccgt tctatcgatg aacaggaaga
    41281 taagccactg aaagtcgaag ggattgatgt ggtttctctg ccgttctata aactgagcac
    41341 caaatttggc gatctcgacc agagcaaaaa ctggctgttg tggtgtgagc gcggggtgat
    41401 gagccgcctg caggcgctct atctgcgcga gcagggcttt aacaatgtga aggtgtatcg
    41461 cccgtaatat gtggttgtaa cgtcgcatct ggtcagatgc gacgcttacc gcatccgaca
    41521 ctactcgtaa taattataaa tccctgccgc catcaccagt tgtgatgcca cttcatgggc
    41581 tttctcgcgc ccaaccagca ggtcgataat tttcagacca aagtcgatag ctgtacccgg
    41641 cccctggctg gtcagcaatt ttacccgtgc atcccagacg acgcgcttgt cctgccattg
    41701 ttcggcggga attttgtctt tcagcgttgg gaagccggtc atattgccaa tcgggaagat
    41761 atcgtgcggg accagcacgg tggctggcgc ggcgcaaata gccgcgacga tacgcccgga
    41821 acggtggaac tgtttaacgg tttcaaccag cagggtgcta tcgcgaaaac actctgcgcc
    41881 tttgatgcca ccaggcagca caatcacgtc atattcgcca tcagccactt cgaccagcgg
    41941 cgcatccgcc agcagcttca cgccgcgcga gcaggtaatc gccaggttac catcgctggc
    42001 gacgctggca gtggtgactt tgataccgcc gcgaaccaag aggtcgatag tggtgacggc
    42061 ttcagtctct tcactaccag gggcgaggca aaccagtgcc gatgcgctca tattcactct
    42121 cctttctttt taccatttca aacaggcggg tgttttccgg tacggcaatc ccatgcgcgc
    42181 gggcgcggcg taagagaaaa ccattgatat agtcgatttc ggtgtggcgc agcgcgcgga
    42241 tatcctgcaa catcgacgag atattttccg ctgttgcatc aatcacctgc atcacgtaat
    42301 cacgcaaatc ttctgctgaa gtatgatgcc cttcgcgttc gatcactgcc gcgacttctt
    42361 cgcatatctg cataatttct tgcggatgat gacgtaattc accattcggg caattccaga
    42421 tggcggtcag tggattaatc acacagttga ctgccagctt gcgccacagc tcggcgcgaa
    42481 tattgttatg ccacgcgacg tcaggcaata cggtttgcaa aatatccgcc agataactgt
    42541 aatcgccgtc ctgttgccgt gccggaccaa tatgcgtgat accgtttgcc acatgaataa
    42601 tgacattgcc gtcgcggcgg gcggcatggg tggtggtgcc catcagtaat ggctgctgaa
    42661 tgttttgcaa ctcttcgatg gtgcccatgc cgttgtgaat taacagtatt ggcgtggtta
    42721 caggcagtgt ggacgcgagg cttttgacgg catcggaaac ctgccatgct ttcagcgtca
    42781 ccaggagcag atcgctggtg gcgagaaaat cgggatcgtt ggcggtcagc gattcattaa
    42841 atatcgaacc atctgtctca accagattca cactacaata aggttgcggt acgcgcagcc
    42901 agccctgaac gtcgtgaccc tgtttacaaa gtgctgtaag ccataattgc cctaaggcac
    42961 cgcatcccaa tacggtaatt ttcattgttc ctcctcaccc gcaaccactc cgggtgttca
    43021 ataaggctat cccttaattg tgcatgctgt tgcgactatg cacaattaag ggatacgtcc
    43081 tggtgcagga ctgttggtta tttaactttg caggtattat gcttcgcatc aaaaatgaag
    43141 ggagaggaaa agatgccatc tttcgatatt gtctctgaag ttgatcttca ggaagcacgt
    43201 aacgcggtcg ataacgcgag ccgcgaagtg gagtcccgtt ttgacttccg taacgttgaa
    43261 gcctcatttg agctgaatga cgccagcaaa accatcaaag tgttgagcga gtccgacttc
    43321 caggtcaatc agttgctgga tattctgcgt gccaagttgc tgaagcgcgg cattgaaggc
    43381 agttcactgg atgtaccgga aaatatcgtt catagcggta aaacctggtt tgtggaagcg
    43441 aaactgaaac agggcattga gagcgcgact cagaagaaaa tcgtcaaaat gatcaaagac
    43501 agcaaactga aagtgcaggc gcaaattcag ggcgatgaga tccgtgtgac gggcaaatct
    43561 cgtgatgatt tacaggctgt catggcgatg gtacgtggtg gcgatctggg tcagccgttc
    43621 cagttcaaaa acttccgcga ttaatcgcga caacctgcgc tttgttcatg tcagatgcgg
    43681 catgaacgcc tgattcggcc tacaaaggtt tgtaactcag gaacttagta ggcctgataa
    43741 gcgtagcgca gcaggcaatt tcgcctcgct tttatgcctg gcgaattgcc tgttcaacct
    43801 caaagcgatt cgtcactttg ctgtcgattt tcacataagc tgaatgttct tcttctgcaa
    43861 tcaacacttc tttgatgcct tcagtttcca gcaaacgcac ttttaacgcc tcgtttgcgg
    43921 caatgttcgc cgggatttca atgcgcaagc tgctgacata cggcggttct ttcatggtac
    43981 tggcgactgc cagccacact gcggccagca ttgcgccagc gagaaatact ccctgaccgt
    44041 caaacatgcc gtcaatccag ccgcccagcg aaccgccaat cgccacgcca agaaactggc
    44101 tggtggagta aacacccatc gccgtacctt tgtaacctgc tggcgattct ttactgataa
    44161 gtgaaggcag aagggcttcc atcaaattaa acgccacaaa gaaaagctgc acgccgacca
    44221 ccagttgcca gaactgcgtt tgtgcgttcc acaacacaat ttccgcaacc acgatcaacc
    44281 cgacgcagaa gacaaagact tgcttcattt tgcgcttaac ttcagcgtag ataatgaaag
    44341 gcacgaccga gccaaaggcg attaacatcg ttgccaggta gaccttccag tgttcagccg
    44401 ccgggaaccc cgcatcagcc atctgtccgg gcagggcaac aaaggtcgac atcagcaaaa
    44461 tatgcagaca cataatgcca aagttgagtt tcagcagccg cggttccgcc agcactttac
    44521 tgaaactgcc ttttaccatt ccggactcac gattaagtac atgagtgcta ctgttgggca
    44581 caacccaaat ggtcaacgca atgccggttg ttgccagaat agcgatcatc cagaacagca
    44641 cgtgcagccc aagtttgtga gtgatgatcg ggccaagcac catcgcaatg gcaaaggtaa
    44701 tgccaaagct cacgccgata aacgccatcg ctttggtgcg gttttgttcg cgcgtgagat
    44761 cggaaagcag cgccataacg gctgcggcaa tcgcaccgga gccttgtagc gcccggccca
    44821 gaataattcc ccagatggag tcagagagcg cagcgataac gctaccggcg gcaaacaccg
    44881 ccagcccacc gacaattaat ggtttgcgac caatgcggtc tgaaagcagg ccaaacggaa
    44941 tctgaaaaac ggcctgagtc agaccataaa taccaatggc aataccgatt aatgcttcgc
    45001 tggcaccttg cagagccatg ccgtacgtgg tcagaaccgg cagaaccatg aacatgccca
    45061 gcatgcgtaa tgaaaatacg gtccctaaac cccaggtcgc gcgccgctca cctggcgtca
    45121 ttttataatc gttcattacc acctctgttt taaattcgcg actagtgtaa agcggcaaac
    45181 gaatggggta aacatgcgga ttttttagta aatatttcga gagggatatg attctaatgc
    45241 taagaaaaaa ggtgccgtag caccttttta actgagaggt tgtgttacca cacagcagcc
    45301 agcagcgtat gcgagtccgg caccataaaa tcaacggaca tcatcaccga gagggcagtg
    45361 atagcgatga tagagaaacc gaacagcttg cgcgcccaga ttctgtcatc agcaacttta
    45421 tagccgcgca gagccatgcc taaccaccag acgctcaccg cagcggcgac caccagatat
    45481 ttatacccag cgtagccgcc aagagagagc atcagcgtgg caacggcaaa ggcgatgata
    45541 tacagcgtga tgtgattctt cgccaccgaa atgcctttca ccaccggcag taccggaatg
    45601 tttgccgcct gataatcctt aaagcggaaa atggctatgg cataagagtg aggcatctgc
    45661 cacaggctga agatagccaa caggatcgct gcgccgctat cgaactcgcc ggttaccgca
    45721 cagtaaccga tcaccggagg cgcagcgccg gagagcgaac caatcaatgt gccgtagaca
    45781 gaatgacgtt tcatgtacag gctataaacg ccgacataaa ccacaaagcc catcaccccc
    45841 agccagcagg ccagcggatt cgcgccaaac cacagcagca taaagccagc aatacccagc
    45901 aaggtggcgt acaccagcga gacagcagga gagatcaggc ctttaaccag cacccgattc
    45961 ttcgtccttt ccatctttct gtcgatatcc ctgtcgatgt agttgttaaa cacacaaccc
    46021 gacgccacaa ccagtgacac cccaaccagc gtgtagataa acaggggata atcaatgctg
    46081 ccctttgagg ccagcaagaa tcccccaatc accgagatca ggttgccaaa gatgatgcct
    46141 ggtttcgtta cttgcaggta ttgcttaaat atcataaccg ccgctcttag tgcatcatca
    46201 tgttgtagtt gaggttccac ataatccaga tggagcccac aaccaggatg gcgatgatta
    46261 gcacggtgaa gacaaacgcc gtcatattcc agccttcatc tgatttggta ttcatgtgca
    46321 ggaagcacac cagatgcacc agaatttgta ccactgccat tgccaggatt gttcccagaa
    46381 ttacggccgg agaggcagcc cctgtcatca ccatccagaa cggaattacc gtcaggatta
    46441 tcgacaggat aaagcctgtc atgtaggtct ttacgctgcc atgggatgcg ccgctgcttg
    46501 cgttagaatg actcattaca tcgcccccat cagataaaca acagtgaaca cacagatcca
    46561 aaccacatcc aggaagtgcc agaacaggct caggcacatg atgcgggtac ggttagtgct
    46621 ggtcaggccg cgacgggcga tttgcaccat cagcaccgcc atccagataa gaccggaagt
    46681 gacgtgcaga ccgtgcgtgc cgaccagcgc aaagaatgct gacaggaagc cgctgcgatc
    46741 cggacccatg ccgttaacaa tcaggtgatg gaattcatag atttccatcc cgataaatcc
    46801 ggcaccaaac aaccatgtca acgccagcca ggagatcacc tggcttttgt tgtttttgta
    46861 catggcgata gccgccatgc cataggtgat ggagctgaac aacagcaaga aagtttcaac
    46921 cagaacgaac ggcagttcga aaatgtcctt acctgtcggg ccgcctgcgg tgccgttcac
    46981 cagaacggca taggtagcaa acaagataga gaacagaatg cagtcgctca tcaggtagat
    47041 ccaaaatccg aaaattttgg ttccgcctgc atcgtggtgc ccgtgttcgt gcgcgtgggc
    47101 agtcgcgtgc gtcaaagtat cagttgccat ttttcagccc tgccttagta atctcatcga
    47161 aatgctggtt ttccagtttt tcgatttctg ccaccggcac gtagtaatcc acgtcctcgt
    47221 cgaagctttt cacgatccag gtgatgatca tgcctgcgaa gccaacaatc gctagccacc
    47281 agatatgcca gatcatggcg aaaccgaaga tggtggagaa agccgcaatg acgataccgg
    47341 caccgctgtt tttcggcata tgaatttctt catagtggtc aggctttttg tacgcttcgc
    47401 ctttctcttt catttcccag aatgcatcac gttcgtgaac gtgcggcaca acggcaaagt
    47461 tatagaacgg aggcggggaa gaggttgccc actccagcgt acggccaccc cacgggtcgc
    47521 cagtcaggtc acggttctgg tcgcggtcac gaatagaaac gtatatctga ataacgaggc
    47581 agagaatacc cagcgcaatc agtaccgcac cgctggctgc aatcatcagc atggtgtgga
    47641 actgcgggtc aatctgctgg ctcaaacgac gggtcatgcc catgaagccc agcgcataca
    47701 gtggcataaa ggcaacgaag aagccgatga tccagaacca gaacgcgcgt ttaccccagg
    47761 tttcgttcag tttgaaaccg aacgctttag gccaccagta ggtcatccct gcgaagcagc
    47821 cgaagaccac gccgccgatg atcacgttat ggaagtgcgc aatcaggaac aggctgttat
    47881 gcagaacgaa gtccgcgccc ggtactgcca gcagcacgcc agtcataccg cctaccgaga
    47941 aggtaacgat aaaaccgatg gtccacagca tcgcagaatg gaacacgatg cggccctgat
    48001 acatggtgaa cagccagttg aagatcttca ccccggtagg gatggcgata atcatcgtgg
    48061 tgataccaaa gaaggcgttt acgttcgcgc ccgcacccat cgtaaagaag tggtgcagcc
    48121 agacgatgaa cgacagcacg gtgatacaga cggttgccca taccagcgag gtataaccaa
    48181 acagacgttt acgcgagaag gttgccgcaa tttcggagaa cacaccgaat acaggcagga
    48241 tcaagatgta aacttccggg tggccccagg cccaaatcag gttgatatac atcatcatgt
    48301 tgccacccat atcgttggta aagaaatggg tgcccagata gcgatccagg gtcaacaacg
    48361 cgacggtaac cgtcagaatt gggaaggaag caataatcag tacgtttgcg cacagtgatg
    48421 cccaggtaaa tactggcatc ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg
    48481 taacgaagaa gttgataccg gtaagcgtcg tacctatacc ggatagctgg agactccata
    48541 tccagtaatc gacaccgact cccggactgt actctattcc cgatagcggt ggataggcca
    48601 gccagccggt ctgcgcaaat tcgcccacgc cgagagaaac gttaaccaga atcacaccaa
    48661 caacggtaaa ccagaagctt aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga
    48721 tctgcagcgg aaccaccagg ttcatcagac cgataacgaa aggcatcgcc acgaagaaga
    48781 tcatgatcac gccgtgtgcg gtgaagatct gatcgtagtg gtgaggtggc aggaagcccg
    48841 cttcgcccgc agaggcaagg gcctgctgac tacgcatcat aatggcgtca gcaaaaccac
    48901 gcagcaacat cacaatcgcc acgatgatat acatgatacc gagacgttta tggtcaacgg
    48961 aggtcagcca ctctttccac aggtaggtcc acttaccgaa gtaagtgatc aggccaacga
    49021 gcgccagacc tcccaaaata atgccagcga tcgtaaccat gacgataggt tcatggaacg
    49081 ggactgcatc aagtgataat tttccgaaca tctttattct tcctcaaccc ctttaatggg
    49141 cggattccgc gtggctcatg tccatgcctt ccataccttc atgtgcgctg tgctcacctt
    49201 ctggctgggt catgtccatg ctcttaccgt gagccataaa cttgttaatc acatcggcaa
    49261 acaagtctgg tttcacgttg gagaaatatt ccacctggtt gtattcgcta ggcgcggcca
    49321 gtttttcgaa cgctgccatg tcagacatgc tgttcggcga ctgtttcgct tttgcgaccc
    49381 attggtcgaa tgcggcgcga tccggtgttg caatagcttt gaacttcatg cctgagaagc
    49441 ccgggccgct gtagctggcg gagataccgt cataagtacc gggttcgttg gcgatcagat
    49501 gcaggcgagt ctgcataccg gccatggcat aaatctggct acccagacgc ggaatgaaga
    49561 aggagttcat cacggagttg gaggtcactt tgaagtacac cggagtgttc gccgggaaag
    49621 cgatttcatt cacggttgca atgccctgtt ccgggtagat gaagaaccat ttccagtcca
    49681 tggaaaccac ttcgatggta atgggcttct cgtcgtgtgc cagcggcttg ctaggctcaa
    49741 gagcgtgagt ggttttccag gtcagtaccg caaggaagat gatgattaag ataggtaccg
    49801 tccagaccac agcttccact ttattggagt gtgaccagtt cgggctgtac ttagcatctt
    49861 tattgctcgc acggtacttc caggcgaaac caacagccat caagattgcg ggaataacga
    49921 caatcaacat caggccaaat gccgtcagta tcagtgaacg ttgctccaga ccaatctgtc
    49981 ctttgggatc taacagcgca gaattacagc cactgagcaa tacagtgcct gcaaataatg
    50041 acaaccatcc caaactttta ttgtatttcc tgagtctcat ttaacgacct caattccacg
    50101 ggatctggtg gcgtttaaag tgtgtgggca ttttacggga aggttacatt actgtaaaca
    50161 tgattaaatt cctgttactt ggatttggct atcaggtcac atatgttgca aaacatatca
    50221 agttgatttt aagaagctgt tgcaaaaggg cgttataacg aaagggaagt gcagaaggag
    50281 tttgggcggg caattggtat aatcaatata aaataaaaca attatttaac aaataactat
    50341 cattagggtg acaactgggt ggtgagaaaa taaggactca tcagctgaat cgtgtaaaaa
    50401 aaatagaatt cataaatggc gtcaataatt tccagacgct aagccgcaca aaagaacaaa
    50461 tatcaataag cgatgctgat gtttatttag ccgtaataat tacggcgagt gattactaca
    50521 gctaaataat atttacagat tacgtcagat gtgtttttcg tagcgccaga taatccagca
    50581 aaccaccaag cacgacgcca gagagggcga ctaaaacccc gacttccagc agcgcaggca
    50641 ggaaagagaa gtgcgtcaaa tccagcgcgt ccatcgtcaa cagcagtaac cacacggcca
    50701 acaggctgat gcccgccgcc agtgtccaca tggcaaaggc ataacctgca ggatattcgg
    50761 tacgggagat aaagttgtca tttactcgtg tatattcaag cgtctggcgg caaaccagca
    50821 gcaaaataag ccctggtacg gcagcggcga cggagaatag atagaatgtc gaccagccgt
    50881 gtgcttcaac aaaccaaccc gccacggggc cgacataaac tcgccctaca gcagaaagcg
    50941 ctgagagcag ggcaaattga gtagcggaaa atgacttatt acacagcgtc attaacagcg
    51001 cgacaaaggc tgatgtaccc atcccgccac agaggttttc gaaaaagacg gctgcaccca
    51061 tgctgtagag atgcttatca gtaatcgaca gcagccagta accggcgtta gatgcacctt
    51121 gtaaaatgcc gaaaatcagc agtgcacgga acagcgacag gcgctgcatc aaaatcccac
    51181 cgtacaatgc gccaacaatg gtcgccagta agccaagcgt tttgttaacc acgcctactt
    51241 cacccgcatc aaacccgacg ccgcgaatca aaaacgtggt tgtcaggctc atagcgaatg
    51301 cgtcgcccag cttatacagc acgataagaa gcaaaataag ccaggcatta ttgcgaccaa
    51361 agaaatctcg cagaggtgca acaaccgctt gttccagcgt tttgggcaca ggaatggtgt
    51421 cggtgggttc tggtgcaagc aacgtcgcga taatacaggg gatcaacagc gccgccatca
    51481 accagtacat gccctgccag cccagccatt tatctgccag ccacagggct aagccgccgg
    51541 aaaccagcat ccctaaacgg taacccagca cgctgattgc cgcgcccgca ccacgttctt
    51601 ctgccgggag cacatcggtt ttccacgcat cgaagacaat atcctgggag gcagagcaaa
    51661 aagcgatcac caccgccagt gccgccatcc agcggagctg ggtgcctggt tcgagaaaac
    51721 ccatcgccgc aatggcgact aataacagaa tttgcgtggc gagcagccaa ccgcgccgcc
    51781 gcccgaaaaa tgggggcgtg tagcggtcca tcagtggtga ccagaggaat ttaaaaacgt
    51841 aagcctggcc taccagagag aagaaaccaa tggttttgag atcgatattc tcgaccgtca
    51901 tccaggcctg taaggtgccg gacgtcaggg cgagcggtag cccggaagca aaacccagga
    51961 tcagcaatat ggctgaacgc ggctgttgaa aaatacgtaa atattgactg gacatgggcg
    52021 tctacaggcc cggcttgcac cgggccagag ggcagaaatt aacgcgcgtt ctgcttgatg
    52081 aattcgtgga tgctggtgtc ctgagacata tcagcgatgg tatcggtcag cacactgtta
    52141 accgcatcgg cgatattttt gttggaggcc tggaacgcac cttcaacgtt gtagctggca
    52201 cgatagtttt tggtcatttt gttgccattc tgcgcggtag cgatgatggc gatatccgct
    52261 ttggtcgcga tgttgtagcg cacgttgccc tgggacacgt cagcatacag ttggctaacg
    52321 atgatttgca gattaaccgg gccattcgga ccaaccatgt atccacgggc ggtcatttgt
    52381 ttttccagca cttcttgcag caggaaacgc agatcgcggg aggcggtcag ggtaacgatt
    52441 tgattatcgc gggtgacttt tgccagcgcc tgatcggtac gctgatcggc accattaatg
    52501 cttacggtga cgcccatcag gcttggatcc tgctgtggca gtgtaatcgt cggggaaact
    52561 tcgatagttg ttggcggttt tgcgcatcct gcaagcataa acagagcaac taacgggaag
    52621 aggatttttt tgaacatgtt cgggctctca gagactctta agcgtgtttg gtaaaaattc
    52681 ccgccatcat aacattgcca acggcgaggg gaagtgggta aggcatgtaa attcatcatg
    52741 ttgacgaaat aatcacccct ggtaaaagaa acgctgatgc gaggcctgtg tttcaatctt
    52801 taaatcagta aacttcatac gcttgacgga aaaaccagga cgaaacctaa atatttgttg
    52861 ttaagctgca atggaaacgg taaaagcggc tagtatttaa agggatggat gacatctcag
    52921 cgttgtcgga ggagatattt catgatgata cgtgagcgga tagaagaaaa attaagggcg
    52981 gcgttccaac ccgtattcct cgaagtagtg gatgaaagct atcgtcacaa tgtcccagcc
    53041 ggctctgaaa gccattttaa agttgtgctg gtcagcgatc gttttacggg tgaacgtttt
    53101 ctgaatcgtc atcgaatgat ttacagtact ttagcggagg aactctctac taccgttcat
    53161 gcgctggctc tgcatactta cactattaag gagtgggaag ggttgcagga caccgtcttt
    53221 gcctctcctc cctgtcgtgg agcaggaagc atcgcgtaaa aacgcatttg caactgtcgg
    53281 cgcttttcca gtatgttgct aaagatttta tgaaaaacgg cctgcgggcc gttttgtttt
    53341 gtctggattt tgcgcttttt gcccagcatt cagacgaaaa tcgcccggga attgtgaaaa
    53401 aatacgcgac agcgcgcaat aaccgttctc gactcataaa agtgatgccg ctataatgcc
    53461 gcgtcctatt tgaatgcttt cgggatgatt ctggtaacag ggaatgtgat tgattataag
    53521 aacatcccgg ttccgcgaag ccaacaacct gtgcttgcgg ggtaagagtt gaccgagcac
    53581 tgtgattttt tgaggtaaca agatgcaagt ttcagttgaa accactcaag gccttggccg
    53641 ccgtgtaacg attactatcg ctgctgacag catcgagacc gctgttaaaa gcgagctggt
    53701 caacgttgcg aaaaaagtac gtattgacgg cttccgcaaa ggcaaagtgc caatgaatat
    53761 cgttgctcag cgttatggcg cgtctgtacg ccaggacgtc ctgggcgacc tgatgagccg
    53821 taacttcatt gacgccatca ttaaagaaaa aatcaatccg gctggcgcac cgacctatgt
    53881 tccgggcgaa tacaagctgg gtgaagactt cacctactct gtagagtttg aagtttatcc
    53941 ggaagttgaa ctgcagggtc tggaagcgat cgaagttgaa aaaccgattg ttgaagtgac
    54001 cgacgctgac gttgacggca tgctggatac tctgcgtaaa cagcaggcaa cctggaaaga
    54061 aaaagacggc gctgttgaag cagaagaccg tgtgaccatc gacttcaccg gttctgtaga
    54121 cggagaagag ttcgaaggcg gtaaagcgtc tgatttcgta ctggcgatgg gtcagggtcg
    54181 catgatcccg ggctttgaag acggtatcaa aggccacaaa gctggcgaag agttcaccat
    54241 cgacgtgacc ttcccggaag agtaccacgc agaaaacctg aaaggtaaag cagcgaaatt
    54301 cgctatcaac ctgaagaaag ttgaagagcg tgaactgccg gaactgactg aagaattcat
    54361 caaacgtttc ggcgttgaag atggttccgt agaaggtctg cgcgctgaag tgcgtaaaaa
    54421 catggagcgc gagctgaaga gcgccatccg taaccgcgtt aagtctcagg cgatcgaagg
    54481 tctggtaaaa gctaacgaca tcgatgtacc ggctgcgctg atcgacagcg aaatcgacgt
    54541 tctgcgtcgc caggctgcac agcgtttcgg tggcaacgaa aaacaagctc tggaactgcc
    54601 gcgcgaactg ttcgaagaac aggctaaacg ccgcgtagtt gttggcctgc tgctgggcga
    54661 agttatccgc accaacgagc tgaaagctga cgaagagcgc gtgaaaggct tgatcgaaga
    54721 gatggcttct gcgtacgaag atccgaaaga agttatcgag ttctacagca aaaacaaaga
    54781 actgatggac aacatgcgca atgttgctct ggaagaacag gctgttgaag ctgtactggc
    54841 gaaagcgaaa gtgactgaaa aagaaaccac tttcaacgag ctgatgaacc agcaggcgta
    54901 atttacgtag cataacgcgc taaattcgca caaaggcccg tcaccgccag gtggtgggct
    54961 tttttttgtc atgaattttg catggaaccg tgcgaaaagc ctctttcggt gttagcgtaa
    55021 caacaaaaga ttgttatgct tgaaatatgg tgatgccgta cccataacac agggactagc
    55081 tgataatccg tccataaggt tacaatcggt acagcaggtt ttttcaattt tatccaggag
    55141 acggaaatgt catacagcgg cgaacgagat aactttgcac cccatatggc gctggtgccg
    55201 atggtcattg aacagacctc acgaggtgag cgctcttttg atatctattc tcgtctactt
    55261 aaggaacgcg tcatttttct gactggccag gttgaagacc acatggctaa cctgattgtg
    55321 gcgcagatgc tgttcctgga agcagaaaac ccagaaaaag atatctatct gtacattaac
    55381 tctccaggcg gggtgattac tgccgggatg tctatctatg acaccatgca gtttatcaag
    55441 cctgatgtca gcaccatctg tatgggccag gcggcctcga tgggcgcttt cttgctgact
    55501 gcaggggcaa aaggtaaacg tttctgcctg ccgaattcgc gcgtgatgat tcaccagccg
    55561 ttgggcggct accagggcca ggcgaccgat atcgaaattc atgcccgtga aattctgaaa
    55621 gttaaagggc gcatgaatga acttatggcg cttcatacgg gtcaatcatt agaacagatt
    55681 gaacgtgata ccgagcgcga tcgcttcctt tctgcccctg aagcggtgga atacggtctg
    55741 gtcgattcga ttctgaccca tcgtaattga tgccagaggc gcaactgtgc cgctatactt
    55801 atccagggcg gcacaacgct gtaagcggct tgcgcctgag aatggcattt gcgtcgtcgt
    55861 gtgcggcaca aagaacaaag aagaggtttt gacccatgac agataaacgc aaagatggct
    55921 caggcaaatt gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg cgcaagctga
    55981 ttgccggtcc atccgtgtat atctgcgacg aatgtgttga tttatgtaac gacatcattc
    56041 gcgaagagat taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg acgccgcatg
    56101 aaattcgcaa ccacctggac gattacgtta tcggtcagga acaggcgaaa aaagtgctgg
    56161 cggtcgcggt atacaaccac tacaaacgtc tgcgcaacgg agataccagc aatggcgtcg
    56221 agttgggcaa aagtaacatt ctgctgatcg gtccgaccgg ttccggtaaa acgctgctgg
    56281 ccgaaacgct ggcgcgcctg ctggacgttc cgttcaccat ggctgacgca accacgctga
    56341 ccgaagccgg ttatgtgggc gaagacgttg aaaacatcat tcagaagctg ttgcagaagt
    56401 gcgattacga cgtacagaaa gcgcagcgcg ggattgtcta catcgatgaa atcgacaaga
    56461 tttctcgtaa gtcagacaac ccgtccatta cccgagacgt ttccggtgaa ggcgtacagc
    56521 aggcactgtt aaaactgatc gaaggtacgg tagctgctgt tccgccgcaa ggtgggcgta
    56581 aacatccgca gcaggaattc ttgcaggttg atacctctaa gatcctgttc atctgtggcg
    56641 gcgcatttgc tggcctggat aaagtgattg ccaatcgtgt tgaaaccggc tccggcattg
    56701 gttttggcgc gacggtgaaa gccaagtccg acaaagccag cgaaggtgaa ctgctggcgc
    56761 aggttgaacc ggaagatctg atcaagtttg gtctgatccc tgagttcatt ggtcgtctgc
    56821 cggttgtcgc aacgttgaat gaactgagcg aagaagctct gattcagatc ctcaaagagc
    56881 cgaaaaacgc cctgaccaag cagtatcagg cgctgtttaa tctggaaggc gtggatctgg
    56941 aattccgtga cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt aaaaccggtg
    57001 cccgtggcct gcgttccatc gtagaagccg cactgctcga taccatgtac gatctgccgt
    57061 ccatggaaga tgtcgaaaaa gtggttatcg acgagtcggt aattgatggt caaagcaaac
    57121 cgttgctgat ttatggtaag ccggaagcgc aacaggcatc tggtgaataa ttaaccattc
    57181 ccatacaatt agttaaccga aaagggggga ttttatctcc cctttaattt ttcctctatt
    57241 ctcggcgttg aatgtggggg aaacatcccc atatactgac gtacatgtta atagatggcg
    57301 tgaagcacag tcgtgtcatc tgattacctg gcggaaatta aactaagaga gagctctatg
    57361 aatcctgagc gttctgaacg cattgaaatc cccgtattgc cgctgcgcga tgtggtggtt
    57421 tatccgcaca tggtcatccc cttatttgtc gggcgggaaa aatccatccg ttgtctggaa
    57481 gcggcgatgg accatgataa aaaaattatg ctggtcgcgc agaaagaagc ttcaacggat
    57541 gagccgggtg taaacgatct tttcaccgtc gggaccgtgg cctctatatt gcagatgctg
    57601 aaactgcctg acggcaccgt caaagtgctg gtcgaggggt tacagcgcgc gcgtatttct
    57661 gcgctctctg acaatggcga acacttttct gcgaaggcgg agtatctgga gtcgccgacc
    57721 attgatgagc gggaacagga agtgctggtg cgtactgcaa tcagccagtt cgaaggctac
    57781 atcaagctaa acaaaaaaat cccaccagaa gtgctgacgt cgctgaatag catcgacgat
    57841 ccggcgcgtc tggcggatac cattgctgca catatgccgc tgaaactggc tgacaaacag
    57901 tccgttctgg agatgtccga cgttaacgaa cgtctggaat atctgatggc aatgatggaa
    57961 tcggaaatcg atctgctgca ggttgagaaa cgcattcgca accgcgttaa aaagcagatg
    58021 gagaaatccc agcgtgagta ctatctgaac gagcaaatga aagctattca gaaagaactc
    58081 ggtgaaatgg acgacgcgcc ggacgaaaac gaagccctga agcgcaaaat cgacgcggcg
    58141 aagatgccga aagaggcaaa agagaaagcg gaagcagagc tgcagaagct gaaaatgatg
    58201 tctccgatgt cggcagaagc gaccgtagtg cgtggatata tcgactggat ggtacaggtg
    58261 ccgtggaatg cgcgtagcaa ggtcaaaaaa gacctgcgtc aggcgcagga aatccttgat
    58321 accgaccatt atggtctgga gcgcgtgaaa gatcgtatcc ttgagtatct tgcggttcaa
    58381 agccgtgtca acaaaatcaa gggtccgatc ctctgcctgg tagggccgcc gggggtaggt
    58441 aaaacgtccc tgggtcagtc catcgccaaa gccaccgggc gtaaatatgt ccgtatggcg
    58501 ctgggcggcg tgcgtgatga agcggaaatc cgtggtcacc gccgtactta catcggttct
    58561 atgccgggta aactgatcca gaaaatggcg aaagtgggcg tgaaaaaccc gctgttcctg
    58621 ctcgatgaga tcgacaaaat gtcttctgac atgcgtggtg atccggcctc cgcactgctt
    58681 gaagtgctgg atccagagca gaacgtagcg ttcagcgacc actacctgga agtggattac
    58741 gatctcagcg acgtaatgtt tgtcgctacg tcgaactcca tgaacattcc ggcaccgttg
    58801 ctggatcgta tggaagtgat tcgcctctcc ggttataccg aagatgaaaa actgaacatc
    58861 gccaaacgtc acctgctgcc gaagcagatt gaacgtaatg cattgaaaaa aggtgagctg
    58921 accgtcgacg atagcgccat tatcggcatt attcgttact acacccgtga ggcgggcgtg
    58981 cgtggtctgg agcgtgaaat ctccaaactg tgtcgcaaag cggttaagca gttactgctc
    59041 gataagtcat taaaacatat cgaaattaac ggtgataacc tgcatgacta cctcggtgtt
    59101 cagcgtttcg actatgggcg cgcggataac gaaaaccgtg tcggccaggt aaccggtctg
    59161 gcgtggacgg aagtgggcgg tgacttgctg accattgaaa ccgcgtgcgt tccggggaaa
    59221 ggcaaactga cctataccgg ttcgctcggc gaagtgatgc aggagtctat tcaggcggcg
    59281 ttaacggtgg ttcgtgcgcg tgcggaaaaa ctggggatca accctgattt ttacgaaaaa
    59341 cgtgacatcc acgtccacgt accggaaggt gcgacgccga aagatggtcc gagtgccggt
    59401 attgccatgt gcaccgcgct ggtttcttgc ctgaccggta acccggttcg tgccgatgtg
    59461 gcaatgaccg gtgagatcac tctgcgtggt caggtactgc cgatcggtgg tttgaaagaa
    59521 aaactactgg cagcgcatcg cggcgggatt aaaacagtgt taattccgtt cgaaaataaa
    59581 cgcgatctgg aagagattcc tgacaacgta attgccgatc tggacattca tcctgtgaag
    59641 cgcattgagg aagttctgac tctggcgcta caaaatgaac cgtctggcat gcaggttgtg
    59701 actgcaaaat agtgacctcg cgcaaaatgc actaataaaa acagggctgg caggctaatt
    59761 cgggcttgcc agcctttttt tgtctcgcta agttagatgg cggatcgggc ttgcccttat
    59821 taaggggtgt tgtaagggga tggctggcct gatataactg ctgcgcgttc gtaccttgaa
    59881 ggattcaagt gcgatataaa ttataaagag gaagagaaga gtgaataaat ctcaattgat
    59941 cgacaagatt gctgcagggg ctgatatctc taaagctgcg gctggccgtg cgttagatgc
    60001 tattattgct tccgtaactg aatctctgaa agaaggggat gatgtagcac tggtaggttt
    60061 tggtactttt gccgttaagg aacgtgctgc ccgtactggc cgcaacccgc agaccggtaa
    60121 agagatcacc atcgctgctg ctaaagtacc gagcttccgt gcaggtaaag cactgaaaga
    60181 cgcggtaaac taagcgttgt ccccagtggg gatgtgacga agttcaaggg cgcatctact
    60241 gatgtgcctt ttttatttgt attcggtgac tttctgcgtc ttgtgggctg acaattgccc
    60301 ccgtttcttg tcacaatagg cctttgcgcg catcgatacg ttgcgtgagg tacacagtca
    60361 tctacagcgg agtgttgtta caccatgatg gacagcttac gcacggctgc aaacagtctc
    60421 gtgctcaaga ttattttcgg tatcattatc gtgtcgttca tattgaccgg cgtgagtggt
    60481 tacctgattg gcggaggcaa taactacgcc gcaaaagtga atgaccagga aatcagccgt
    60541 gggcaattcg agaacgcctt caacagcgag cgtaatcgca tgcagcaaca gctgggcgat
    60601 caatactccg agctggcagc gaacgaaggc tatatgaaaa ccctgcgtca acaggtgctg
    60661 aatcgtctga tcgacgaggc gcttctggat cagtacgctc gtgagctgaa actgggtatc
    60721 agcgatgagc aggttaaaca ggcgattttc gcgaccccag ccttccaggt tgacggcaaa
    60781 tttgataaca gccgctataa cggtatcctc aaccagatgg ggatgaccgc cgatcagtac
    60841 gcccaggcgc tgcgtaacca gctcactacc caacagctga ttaacggcgt tgccggtacc
    60901 gattttatgc tgaaaggtga aaccgacgag ctggcggcac tggtagctca acaacgcgtg
    60961 gtgcgtgaag cggctatcga tgttaacgcg ctggcggcga agcagcctgt gaccgaacag
    61021 gaaattgcca gctactacga acaaaacaaa aacaatttca tgacgccgga acaattccgc
    61081 gtgagttaca tcaagctgga tgccgcgacg atgcagcaac cggttagcga tgcggatatc
    61141 cagagctact acgatcagca tcaggatcaa ttcacccagc cgcagcgtac ccgttacagc
    61201 atcatccaga ccaaaactga agatgaagcg aaagcggtac ttgatgagct gaataagggc
    61261 ggtgattttg ctgcattagc caaagaaaaa tctgccgata ttatctctgc ccgtaacggc
    61321 ggtgatatgg gttggttaga agatgccact atcccggacg aactgaaaaa tgcgggtctg
    61381 aaagaaaaag gccaactgtc tggtgttatc aaatcttcgg tcggtttcct gattgtacgt
    61441 ctggacgaca ttcagccagc gaaagtgaaa tcgttagacg aagtacgtga cgacattgcg
    61501 gcgaaagtga aacacgaaaa agccctcgat gcgtactacg cgctgcagca gaaagtgagc
    61561 gatgcggcaa gcaacgacac cgagtctctg gccggtgcag agcaagctgc cggcgttaaa
    61621 gccactcaga cgggttggtt cagcaaagat aacctgccgg aagagttgaa cttcaagcca
    61681 gttgccgacg ctatcttcaa cggcggtctg gtaggtgaaa acggcgcgcc gggcatcaac
    61741 tctgacatca tcaccgtaga cggcgaccgc gcattcgtgc tgcgcatcag cgagcacaaa
    61801 ccggaagcgg tgaaaccgtt ggcagatgtt caggaacaag ttaaggcact ggttcagcac
    61861 aacaaagctg aacaacaggc gaaagtggat gctgagaaac tgctggttga tttgaaagcc
    61921 ggcaaaggtg cggaagcgat gcaggctgcc ggtctgaaat ttggcgagcc gaaaacctta
    61981 agccgttccg gtcgtgaccc gattagccag gctgcgtttg cactgccgct gccagcgaaa
    62041 gacaaaccga gctacggtat ggcgaccgat atgcaaggta atgtggttct gctggcgctg
    62101 gatgaagtga aacaaggttc aatgccggaa gatcagaaaa aagcgatggt gcagggtatc
    62161 acccagaaca acgcacaaat cgtctttgaa gctctgatga gtaacctgcg taaagaggcg
    62221 aaaatcaaaa ttggcgatgc gctggaacag caataatcct gaagccgcct cgcaaaaaaa
    62281 tgctttgcag ctgtaacaag aaaaggtcgc tttcgcggcc ttttccattt ctgaacattg
    62341 ccatttgttt actgttttca ctgccgttaa ggtgattcca ctgttaacaa acaaggagaa
    62401 aacagtatga aacacggaat taaagcactg ctcattaccc tgtccctggc ctgtgccgga
    62461 atgtctcata gtgctctggc ggcagctcct gcggcaaaac cgacaacggt agaaaccaaa
    62521 gcggaagctc ctgcagcaca aagtaaagca gcagtaccgg cgaaagccag tgacgaagaa
    62581 ggcacccggg tcagcattaa taatgccagc gcggaagagt tagcccgcgc gatgaatggc
    62641 gttggcctga agaaagcgca ggcgattgtc agttatcgcg aagagtacgg tccgtttaaa
    62701 acggtggagg atctaaagca ggtgccgggg atgggcaatt cgctggtgga acgtaatatg
    62761 gcggtattaa ccctgtaatt aatttgcata gtggcaattt tttgccagac tgaagaggtc
    62821 ataccagtta tgacctctgt acttataaca gttcttataa cagttcttat aacaacaacg
    62881 taaggttatt gcgctatgca aacacaaatc aaagttcgtg gatatcatct cgacgtttac
    62941 cagcacgtaa acaacgcccg ctaccttgaa tttctcgaag aagcccgctg gcatgggttg
    63001 gaaaatagcg acagttttca ctggatgacg gcccataaca tcgctttcgt cgtggtcaat
    63061 atcaatatta actatcgccg cccggcggta ttaagcgacc tgttaaccat taccagtcag
    63121 ttgcagcaat taaacggtaa aagtggcatc ttaagccagg tcattacact ggagccggaa
    63181 ggacaggtgg tagcggatgc gcttattacg tttgtttgta ttgatcttaa aacgcagaaa
    63241 gcattagctc tggaagggga attgcgcgaa aagctggagc agatggttaa gtaaacgttt
    63301 tgtggtgccg gatgctcaag ccgcatccgg cgacacccgg aataattact tcaacccggt
    63361 tttctgcttc atcgctgcca tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt
    63421 ggcacgtaaa ttacacgccg cacaatgacc gcaaccgtcg cctttaatgc cgttataaca
    63481 ggtcagcgtt tcgttacgga ctaaatccag cttgccgtaa taatctgcca gcgcccaggt
    63541 ttccgcttta tcaatccaca tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa
    63601 actgacggca tggtttagtg ctttcacaaa ctcatcgcga caatccgggt agccggagaa
    63661 atccgtttcg cagacgccgg taattacggc ttctgctttt acctgatacg catatattgc
    63721 cgccagcgtc aggaacaaaa tattacgccc tgggacaaac gtattcggga taccatcggc
    63781 ttcaggttca taatcaggca ccggaatgct gtcacgcgtc aggctgctga ccgccagctc
    63841 gttgagcagc gttacatcaa gaaccttatg cgcgcgtgcc cccagtttca gcgccagttc
    63901 gcgtgccacg tcgatttctg cgcgatggcg ttgaccgtaa tcgaacgtca cgcaatggac
    63961 ttcatcatat tgttgtaatg cctgcaccag acaggtggtg gaatcctggc ctccactgaa
    64021 cacaacgaca gcacgtttca tagataatcc tgcttgaaca ataaaagcgt tatggtaacg
    64081 cctgcgatta acccggacca gctattcatt gcgacggcgc gggaagccag gcttcggtaa
    64141 attcaaacca gccgcgcggt gtcagtcgca caccgttcac gccgggaggg gcgctaatgc
    64201 gatagtgata gttgaacagc ggcgtcagcg tcgcatctgc cattaactgg ctaaaaaccg
    64261 ctttcagggc attaaatcgg ttttcctcat caggcattac ttgcactgcg tccagtgtcg
    64321 attgcaaatg tgcatatgct ggagcgtcga aaacatgtgg ccacagtgga tcgcaacgca
    64381 gccattgctc cagggtatat tccggtgctt cgccaattaa tctgtcgccc atcatgaggt
    64441 ctgcgtgtgc cagtagggtc gtgtcgtccc agttttttgc gttatgaaaa ataattgtga
    64501 gttcacagcc ttccgctgcc agtgtcgcct gtaggcgttc tgccatggta tgaagttcta
    64561 tcggtaggtg ataaaccagc gtcaaggttt tcggtagttt aacttcatcc ggtacctgcc
    64621 aatgcggaat agtccagcca ggcagtaatg catgactggc ggtgatcagg ttttctccga
    64681 cttctaacgt ttgtaataaa ccggattgat gaataatgga gattactttt cgcgcctgcc
    64741 agagggacag tcggagactt ttgcgcaatg ttaaatagca aaaacctaaa ctgatgccgc
    64801 tactgacctg gctgacccgt tgcagctcct ccggtttgcc aatggtgatt tgcacgggat
    64861 gccgacaact ggttcccaaa tctttttcga aaagcggcgg agttatccag tactcaaccg
    64921 ctttaagcag cggatgacgt aaatggtaat aatcatggct ctccaggcgc accagttccg
    64981 ctgtgaattg tgttaagcga aaaggacctg tgccgatcag tgggaattgc ggatgcgcca
    65041 gatggctgca atagctcgcc agtcggtgcg caagccagta atcggggcga tgtaaaaaga
    65101 aggtcagaca ctgcggatgg gtgacttcaa tacgcttcac gctaataaat aattgatcca
    65161 gtgctggcag ttgtaacagc atcaataatc gctggtgtaa gtgtgaggct tttactgcat
    65221 cgccgttatg ccagtgtagc gttgaacgaa gataaaagtc ccagcgtaac ccgtcagtag
    65281 aggtttccca gtgatgtgct aaatcgccaa tcgggcgctg agtattatta tcgaagcggg
    65341 tcaggccgga aaatatctgc ccggccagat gctgctcggc acggccgggc aaaaagcccg
    65401 gttgtagcgg ttcgagcggg cgatagtagg gaatacgcaa cgtgggcgta tcgttttgcc
    65461 attgtccgcc cataaacggc tgtaacagag tgcgcagctc acctggggcc agttgcgcca
    65521 gctccagcac atcttgctgc tttccggttt ccagcgcctg ttccatcatc gcattgcgta
    65581 gcgattctgg cgtgaccaga aagcgtagtt gaccgcgctt tccgcgtcct gactgcgcct
    65641 gccactccag ccatcccgcc tcctgtgcct gacgcaacac cgtacgaaca tggcgttcgc
    65701 tgcaaaaaca gcgttcggcc agttcgctga cggtgacggt ttgcggcttt ccggcggaag
    65761 gttgccacag acgttgatac tggttaagac ggttgagcag tcgcatataa acccggaaca
    65821 atattattta actattcact attacttccg tatatatcag gtgatactca atcaccatta
    65881 actgtgtcac agagtggaga aagaaatggc tcgtctggca gcatttgata tggatggcac
    65941 tttattgatg cctgaccatc atttaggtga gaaaaccctc tctactttgg cgcggctgcg
    66001 tgaacgcgac attaccctca cttttgccac ggggcgtcat gcgctggaga tgcagcatat
    66061 tctcggggcg ctatcgctgg atgcatattt gattactggc aacggaacgc gcgtgcattc
    66121 tctggaaggt gaacttttac atcgtgatga tttacctgcg gatgtcgcgg agctggtgct
    66181 gtatcagcaa tgggataccc gagcaagcat gcatatcttc aatgacgatg gttggtttac
    66241 cgggaaagag atccctgcgt tgttgcaggc atttgtctat agcggttttc gttatcagat
    66301 aatcgatgtc aaaaaaatgc cactcggcag cgtcaccaag atctgcttct gtggcgatca
    66361 cgacgatctt acacgcttgc agatccagct atacgaagca ttaggcgagc gtgcacattt
    66421 atgtttttcc gccacggatt gcctcgaagt gctgccggtg ggctgcaata aaggcgctgc
    66481 attgacggtg ctgacccaac atttaggttt atcgttacgc gattgtatgg cctttggtga
    66541 tgcgatgaac gatcgcgaaa tgttaggcag cgtcggtagc ggatttatta tgggcaatgc
    66601 gatgccgcaa ctgcgcgcgg agctcccgca tttaccggtg attggacatt gccgaaatca
    66661 ggctgtctct cactatttga cgcactggct ggactatcca catctacctt attcccccga
    66721 ataacgagat cccttccagc accgggcaat tgcccggttt tttttgcgtt gaatttgtca
    66781 ttttgtgccg tggtgcttaa accgcacaga ataaattgtc gcgatttcac ctttaaaata
    66841 gaattaaaag agaaaaaaat tctctgtgga agggctatgt tagataaaat tgaccgtaag
    66901 ctgctggcct tgctacagca ggattgcacc ctctctttgc aggcactggc tgaagccgtt
    66961 aatctgacaa ccaccccctg ctggaagcgt ctgaaacggc tggaggacga cggtattctt
    67021 atcggcaaag tcgccctgct ggatccggaa aaaataggcc tcggcctgac cgcctttgtg
    67081 ctgataaaaa cgcaacatca cagcagcgaa tggtattgcc gctttgtcac ggtggttacc
    67141 gaaatgccag aagtgctggg gttctggcgc atggctggtg aatacgatta tctgatgcgc
    67201 gtccaggttg ccgacatgaa acgctacgac gagttttata agcgtctggt aaacagtgta
    67261 ccggggctgt cggacgtcac ttccagcttc gcgatggaac agattaaata caccacttct
    67321 ttacccatcg aataaatatc cagaatcaag tcaggacaca acgcgtgcga ttatttgctc
    67381 aattaagctg gtatttccgt cgggaatggc gtcgctatct cggggctgtc gccttgcttg
    67441 tcattatcgc gatgctgcaa ctggttccgc caaaagtggt tggtattgtt gttgatggcg
    67501 tgacagaaca acactttact accgggcaga tcctgatgtg gatcgccacc atggtgctga
    67561 ttgccgttgt ggtttatctc ctgcgttacg tctggcgcgt attgctgttt ggtgcgtctt
    67621 atcaactggc tgttgaactg cgtgaagatt attaccgcca gctaagccgg cagcatcctg
    67681 agttttacct gcgtcatcgc acgggcgacc ttatggcccg tgcgacaaat gacgtcgatc
    67741 gcgtcgtgtt tgccgccgga gaaggggtgc tgacgctggt ggattcactg gtaatgggct
    67801 gcgctgtgtt gattatgatg tctacgcaaa ttagctggca gttgacctta tttgccctgt
    67861 tgccgatgcc agtgatggcg atcatgatca agcgcaacgg cgatgccttg catgaacgct
    67921 ttaagctggc acaggcggcg ttttccagtc ttaatgaccg cacccaggaa agcctcacca
    67981 gtatccgcat gatcaaagcc tttggtctgg aagatcgtca gtcggcgtta tttgccgcgg
    68041 atgccgaaga taccggcaaa aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc
    68101 cgaccatcta tatcgcgatt ggtatggcga acttgctggc gatcggcggc ggtagttgga
    68161 tggtggtgca gggcagttta acgctgggcc agctcaccag ttttatgatg tatttaggtc
    68221 tgatgatttg gccaatgctg gcgctggcat ggatgtttaa cattgtggaa cgtggcagcg
    68281 cggcgtatag ccgaattcgt accatgctgg ccgaagcgcc agtcgttatt gatggtagtg
    68341 ataaggtgcc agaaggtcgt ggtgaactgg atgtgaatat tcgccagttt acgtatccgc
    68401 agactgacca tcctgcgctg gaaaacgtca atttcgccct gaaacccggt cagatgctgg
    68461 gtatctgcgg gccgactggt tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt
    68521 tcgacgtcag cgagggtgat atccgttttc atgatattcc tctgacgaag ttacaactcg
    68581 atagctggcg tagccgtctg gcggtggtca gccagacacc attccttttt tccgacaccg
    68641 tggcgaacaa catcgcgttg ggttgcccga atgccaccca gcaagagatt gaacacgtag
    68701 cgcggttagc cagcgtacat gacgatattt tgcgtctgcc gcaaggttac gacacagaag
    68761 tgggcgagcg cggtgtgatg ctttctggcg gacaaaaaca gcgtatctcc attgctcgag
    68821 cgttattagt caacgcggaa atcctcatcc ttgatgatgc gctttcagcg gtggacggac
    68881 gcacagagca ccagatcctg cataacctgc gtcagtgggg gcagggaaga acggtaatca
    68941 tcagtgccca tcgcctttct gcactgacgg aagccagtga aattattgtg atgcagcacg
    69001 ggcatatcgc ccagcgtggc aatcatgatg tgctggtaca acaaagcggc tggtatcgcg
    69061 atatgtatcg ctatcaacaa ctggaggcgg cgctcgacga cgccccggaa attcgcgagg
    69121 aggccgtcga tgcgtagttt tagccaactg tggccgactc tcaagcgtct gttagcgtac
    69181 ggttcaccgt ggcgtaaacc gttggggatt gcggtcctga tgatgtgggt tgcggcggcg
    69241 gcagaagtca gtgggccgct gcttatcagc tattttatcg acaatatggt agcgaaaaat
    69301 aacctgccgt tgaaagtggt tgcagggctg gcggcggcgt atgttgggct gcaactgttt
    69361 gcagccgggc tacattacgc gcagtcgctg ctgtttaatc gggcggcagt aggcgtagtg
    69421 caacagttgc gtaccgacgt gatggatgct gcgttacgcc agccattaag cgagtttgat
    69481 acccaacccg tcgggcaggt gatttcccgc gtcactaatg acactgaagt gatccgcgat
    69541 ctctacgtta ccgtagtggc aactgtcctg cgtagcgccg cactggtggg cgcgatgctg
    69601 gtggcgatgt tcagcctcga ctggcgaatg gcgctggtgg cgataatgat tttcccggtg
    69661 gtgctggtgg taatggtgat atatcagcgt tacagcacgc cgattgtccg tcgtgtgcgc
    69721 gcttatctgg cggatatcaa cgacggcttt aacgaaatca tcaatggcat gagcgttatc
    69781 cagcagttcc gtcagcaggc gcgatttggc gaacgtatgg gtgaggccag tcgttcacac
    69841 tatatggcga ggatgcaaac cctgcgcctc gacggttttc tgctgcgtcc gctgctgagt
    69901 ctgttttcat cgctcattct ttgtggcttg ttgatgctgt ttggcttctc cgccagcggc
    69961 accattgaag tgggcgtgtt gtatgcgttc atcagttatt tggggcgact taacgaaccc
    70021 ttaatcgaac tgaccacgca gcaggcgatg ctgcaacagg ctgttgttgc tggtgagcgc
    70081 gtttttgagc tgatggacgg accgcgccag caatatggca atgatgatcg ctcgttgcag
    70141 agcggcacca tcgacgtcga taacgtgtca tttgcttatc gcgatgacaa tctggtgcta
    70201 aagaacatta atctctctgt gccttcgcgc aactttgtgg cgctggtcgg gcataccggc
    70261 agtggcaaaa gcaccctcgc cagtttattg atgggctatt acccgctaac ggaaggtgag
    70321 attcgccttg atggtcgtcc attaagtacg ctaagtcaca gcgcgctgcg ccagggcgtg
    70381 gcaatggtgc agcaagatcc ggtggtgctg gcggatacct tcctcgccaa cgtgacgctg
    70441 ggacgtgata tctctgaaga acgcgtctgg caggcgctgg aaaccgtgca gctggcggag
    70501 ctggcgcgta gcatgagtga tggtatttac acgctgcttg gcgagcaggg gaataatctc
    70561 tcagtcgggc aaaagcaact gctggcactg gcgcgcgtgc tggtggagac gccgcaaatc
    70621 ctgatccttg atgaggcaac cgccagcatt gactccggga ctgaacaggc gattcaacat
    70681 gctctggcgg cggtgcgtga acatactacg cttgtggtga ttgctcaccg cttatcgact
    70741 attgttgatg ccgacaccat tctggtgctt catcgtgggc aagccgtgga gcagggcact
    70801 caccagcaac tgctggcggc ccagggacga tactggcaga tgtatcaact gcaacttgcg
    70861 ggcgaagagc tggcagccag cgtgcgtgaa gaggaatcat tgagcgcctg aatagcgcaa
    70921 tatttcatcg ttggtgcaaa aatgtaacgc actgtgcact gtcatagtgc gttttcattt
    70981 tcaatcttct taacttcctg ctctctttct cgtttttcat ttctggcaca ccgcttgcaa
    71041 taccttcttc gtgtagcaga accattaccg aattctgacc ggaggggatc tatgaagctg
    71101 gtgaccgtga taatcaaacc attcaagctg gaagacgttc gtgaagcgtt atcttccatt
    71161 ggtattcagg gcctgaccgt caccgaagtg aaaggttttg ggcgtcagaa agggcatgcc
    71221 gagctgtacc ggggggcgga atacagcgtc aacttcctgc caaaagtaaa aattgatgtg
    71281 gcgattgctg atgaccaact cgatgaagtg atcgatatcg tcagcaaagc ggcttacacc
    71341 ggaaaaattg gcgacggcaa aatcttcgtc gctgaattgc aacgcgtcat tcgtattcgt
    71401 accggcgaag ccgacgaagc ggcgctgtaa tctctggcac acagcaacag gaacgaaaaa
    71461 tgaagatagc aacgataaaa actgggctta cttcactggc gatgctgccg ggactggtaa
    71521 tggctgcacc tgcggtggcc gataaagccg acaatgcatt tatgatgatt tgtactgcgc
    71581 tggtgctgtt tatgactatt ccggggattg ccctgtttta tggagggctg attcgtggca
    71641 aaaacgtgct gtcgatgctg acgcaggtga ccgtgacgtt tgcactggtc tgtattctct
    71701 gggtggttta cggctactcg ctggcgtttg gcgaaggcaa caacttcttc ggcaacatta
    71761 acgggttgat gctgaaaaac attgaactga cggcggtgat gggcagcatt tatcagtata
    71821 tccacgtggc gtttcaggga tcgtttgcct gcattaccgt aggcttgata gttggggcgc
    71881 tggcggaacg aatccgcttc tcagctgtgt tgatttttgt ggtggtatgg ctgacgctct
    71941 cttacattcc gattgcgcat atggtctggg gcggcggttt gctggcttct cacggtgcgc
    72001 tggatttcgc gggtggcacg gtagtgcaca ttaacgccgc aatcgccggt ctggtgggcg
    72061 cgtatctgat aggcaaacgc gtgggcttcg gcaaagaggc gtttaaaccg cacaacctgc
    72121 cgatggtctt caccgggact gccattctct atatcggttg gtttggcttt aacgccgggt
    72181 cagcgggcac ggcgaatgaa atcgcggcac tggcatttgt gaatactgtg gtcgcaaccg
    72241 cggcggcaat tcttggctgg atcttcggtg aatgggcgct gcgtggtaag ccttcactgc
    72301 tgggagcgtg ttctggcgcg attgccggtc tggtcggcgt gacgccagcc tgtggttaca
    72361 ttggggttgg cggcgcgttg attatcggcg tggtagctgg tctggcgggc ttgtggggcg
    72421 ttaccatgct caaacgcttg ctgcgggtgg atgatccctg cgatgtcttc ggtgtgcacg
    72481 gcgtttgtgg tattgtcggc tgtatcatga ccgggatttt tgccgccagc tcgctgggcg
    72541 gcgtgggctt cgctgaaggt gtgacgatgg gccatcagtt gctggtacag ctggaaagca
    72601 tcgccattac gattgtctgg tctggtgtgg tggcatttat cggctacaaa ctggcggatc
    72661 tgacggttgg tctgcgtgta ccggaagagc aggagcgaga agggctggac gtcaacagcc
    72721 acggcgagaa tgcctataac gcgtaacaag cactgcaaaa aacagccgga cggttttcac
    72781 ctccggctat ttttttaatt gtgattacgc atcacccctt cctgaacggt cgaggcaacc
    72841 agcacgccgt cctgggtata aaactcaccg cgcacaaagc cgcgtgcgct ggacgccgag
    72901 gtgctttcca cgctatacag cagccattca ttcaaattaa acgggcgatg gaaccacatg
    72961 gaatggtcaa tggtggcaat ctgaatcccc ggttcgagaa aaccgatgcc gtgcggctgt
    73021 agagctaccg gcaggaaatt aagatcagaa gcataaccga gcagatactg atgaacgcgc
    73081 agatcatccg gcacgctacc atttgcgcgg atccacacct gacgatgcgg ttctgcgacg
    73141 tgacctttca gtgggttatg aaactccacc ggacggactt ccagcggacg atcgcagatg
    73201 aatttatctt tcagcactgg cggcagcagg tgcgccagcg attgggcgat ttgcgtttct
    73261 gaaggcaggc catcaggcgc tggtgcggac ggcattgttt tttgatgttc gaaacccgct
    73321 tctggtgcct ggaaagaggc agtcatataa aaaatcggtt tgccgttttg aatagcagca
    73381 acccggcggg cgctgaagct gttaccgtca cgcagtgttt cgacatcata aataatcggc
    73441 ttcttactat cgccagggcg aagaaagtag ctgtgaaaag aatgtaccag ccgctcgtca
    73501 gggacggtct cttttgcagc atacaaggcc tgacccacga cctggccgcc aaatacctgg
    73561 cgtaaaccta aatcttcact ctggccgcga aagagtcctt cctcaatttt ttccagattt
    73621 aacaatgtca gtaaattttt tagcgcctga ctcatataac tctccagtaa caaagctgcc
    73681 gcagcaagcc aaagtgagtt gagtataacg caaatttgcc actggtccga tgggtgcaat
    73741 ggtctgaatt acgggctaat tacaggcaga aatgcgtgat gtgtgccaca cttgttgacg
    73801 ttactatttt gttaaccact cttccggcga ggaaagttag cccgctggtg cattgataat
    73861 aaggagaagt gaatgaaact cgtgcacatg gccagtggtt tagcggttgc gattgcgttg
    73921 gcggcttgcg cagataaaag cgcggatatt cagacgccag caccggctgc aaatacgtct
    73981 atttcagcaa cacaacaacc tgctatccag caaccgaatg tctccggtac cgtttggatc
    74041 cgtcagaaag tcgcactgcc gcctgatgcc gtgctgaccg tgacactttc tgacgcgtcg
    74101 ttagccgatg cgccgtcgaa agtgctggcg cagaaagcgg tgcgtaccga aggtaaacag
    74161 tcaccattca gctttgttct gccatttaac ccggcagatg ttcagccgaa cgcgcgtatt
    74221 ctgttgagtg cggcgattac cgtgaatgac aaactggtat ttattaccga taccgttcag
    74281 ccggtgatca accagggcgg aactaaagcc gacctgacat tggtgccggt gcagcaaacc
    74341 gccgtgccgg ttcaggctag cggtggcgca acgactaccg taccttcaac ttcaccaacc
    74401 caggtgaatc cgtcttcggc agttccagct ccaacgcaat attaagcgaa gttaaccctc
    74461 tccgcccgga gagggttagt agttccagcg ataaagctgc aagtcgattt gcccgcttcc
    74521 cgacaccatc acaccttctg ccagtaatgc ctgtcgctga cgctgtaaat ccggtccggt
    74581 cagtgaaatg gtgccgtggc gattaaccac ccggtgccag ggcaaggtgc tgccttcggg
    74641 gagacgcttt aacacaccgc caacctggcg cgcggcacgg ggcgagcccg ccagtttcgc
    74701 cacatcaccg taagtggtga catagccttc gggaatagcg gctacgattt gccagacgcg
    74761 ttggggaaat gaatcttctt tttccatctt ttcttcctga ggtaattttt cagcataatc
    74821 tggaaaaacg cccgagtgaa gccgcattgc gcaagaaacc agcatctggc acgcaatggg
    74881 ttgcaattag ccggggcagc agtgataatg cgcctgcgcg ttggttctca acgctctcaa
    74941 tgggggctct gttggttctc ccgcaacgct actctgttta ccaggtcagg tccggaagga
    75001 agcagccaag gcagatgacg cgtgtgccgg gatgtagctg gcagggcccc cacccatttc
    75061 tgcctcctac catttcgtca aaaaatccca acatggctaa actttaacca caactgacgt
    75121 cgcaagaatt gtctggctgc gcagtacgct tcggaggtat gtctgatgaa gtatgttgat
    75181 ggttttgttg ttgccgttcc tgccgataaa aaggatgcct atcgggaaat ggccgctaag
    75241 gccgcgccat tatttaaaga gttcggggcg cttcgtattg tcgaatgctg ggccagcgat
    75301 ataccggatg gtaaagtgac cgattttcgt atggcggtga aagcggaaga gaatgaagag
    75361 gttgtcttta gctggattga atacccttca aaagaggtcc gcgacgctgc taatcaaaag
    75421 atgatgtcgg acccacggat gaaagagttc ggcgagtcca tgccgtttga tggtaagcga
    75481 atgatctatg gcggattcga gtcaatcatc gacgagtagg tagcgtgacg ggctgtgcgg
    75541 cgtgccgtca gccctataat tcacaaatgt tgctcggccc agcgtaagaa atcctctttc
    75601 ggtaatgcct tgctgtagag ccagccctga ccgtagtgca cgccatgctg gcgtagccac
    75661 tcttcttgtt tactggtttc gattccctcc gcaaccattt tcagtttcag tgtttttgcc
    75721 atttcgatga tatgtggcgt gacattctta tattccagcg catcaacgaa agatttatcg
    75781 atcttcagaa tgtcgacatc cagatcctgt aaatagctta aacttgaata ccctgtacca
    75841 aaatcatcga gataaatttc atggcccgcc tcccggtagc gagaaattat cggggcgctg
    75901 gttttcggat cggcaaactc gcgttcagtg agttcaagcg cgatctgtct gggattaacc
    75961 tgatagtgat tgatcatttc acgcagcaat tgcgggattt tttccgaggt gagcacggtg
    76021 gattcaagat tgatcgaaat atgctgctga ggatgctgac gcagccagtc gcccatatct
    76081 tcaaagacgc ttcttataat cagtagcgtc aatggctcag aaaggcccgt ttgctgtgcc
    76141 agcggaataa aactatctgg tgacaaccaa ctaccgtctg tctgcggcca gcgcgccagt
    76201 gcctcagcac cgacaatttt gccattggct aaggagacaa tcggctgata gtgcacgcaa
    76261 atatcacgat tttcgatagc atcctgcagc cgatgatgcg gtgactggat acggcgcaaa
    76321 atacgcagca caaacatcgc tgccagcagg ccaatcacca acccgagcgg taaccaaata
    76381 aagacttgcc gataccagcc tttctgcaac attttcgttg aagcccaggt gatgatcgaa
    76441 atattcatct ccggtaaggg caggatatcg taaatgattc cattattttc gatatgctca
    76501 ccgggtgttt tttgtagcct ggtaataatt ccctgagcaa gttcatcgct gctggttatg
    76561 acaacgttat gggcattgcc aataatggcg gcatcaattt gccatgagct atagggaatg
    76621 acatcaataa aggaagcggg gtcgatcatg acgacataat gtgccgttcc catggcgacc
    76681 atgtaacgga taatgcctaa atcgttatgc gatgttaacc agacacgata accatctttc
    76741 gaaattttac ctggctcggg gaaggtatcg ggcgggcttt catgctccag agacgaacat
    76801 tggggaacgt tgttatcgat ataaaccact tcctgaatat agcgataact gtaagatacc
    76861 cgacgcattt ccatgagatg ggcttcgcta caggcagcgc cttgccatct ttccagctct
    76921 tgcagcgcat ctttcccttg tgtcgccacc ttattggccc gaatagcgac gcgggaggaa
    76981 tacgtattca gctcttcaat aaatgacgtt tctacctgct gatgggccag ccagatgctt
    77041 aagccgacag gcagcaatac tgaaagaatc agtactcccg aaataaggcc gaccagatgt
    77101 cgtgttctca ccagcatatc cttattaaca cagtgtaggg aaagtatatc tgattgtgga
    77161 aaaaggctgg caggagataa ggaggtagag ctggtatttc atgaagtgat gggtggaagc
    77221 aatcgttgtc atttgaccag gcgtagagct ttacgcctgg tatgtacccc taacttaaga
    77281 tgttgattcg gctcgagcga gggtaaaaat atcgtaaaaa gatagttcac ctttacggac
    77341 gatcattttt tgcagttgtt ctttatgaac atccgcgaca cgtggagagt tcaggatggc
    77401 ttcaaccagc gcggcaggta caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg
    77461 gaaatccagc acgtcgatat cttcatagcg gtagcgtggg tagacatcga aaaagaagaa
    77521 accattaagc aggataaata atacaaccag tagccagaca gagcctgaaa gcgtttcgga
    77581 ttgcaacatc accgccaggg tagcaaaaaa agcaacgtac ataccgataa acagccccgg
    77641 atgtttacgg ataaaactga tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt
    77701 cagagactca atcgtttcgg tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtga
    77761 tatctgcaac tttccaggga acaccctatt ttaacgtggg aatagtagag gggaagtaac
    77821 agatttgcca cgattttgca taacagttga gaaaagctgg ttacaatttt ttaatgattc
    77881 tggctggatt accgcccacg acaacgttgt ccgggacatc ttttgtgaca actgcacctg
    77941 aggctaccac gacgttatca ccaatggtca caccagggtt aatgactgca cgtccgccaa
    78001 tccagacgtt attaccgatg gtgacgggtt tccccagttc agcaccgcta ttacgtgcta
    78061 cagggtcgat gggatgtgtt gccgtgtaga tatgaacgcc tggtgccaac atacagttat
    78121 caccaatgcg aatagggcag acatcaagca tcacgcaatc gaagttggcg aaaaaattat
    78181 tgccgagaaa aatgttatag ccatagtcac agcgaaacgt tggctcaata taagcctctg
    78241 tcacctgacc gaataaatca gcaagaattt gctggcgtaa tgtgtgctct tccgccaggg
    78301 aatgattgta tcggtgaata agccgacgag cgcgcaggcg atcgcgagat aacgcctcat
    78361 ctgccgagtg atacaactca ccagcaatca tcttttcttt ttctgtgctc attactgact
    78421 tccggttgct gaaaatgcgg caacacagta agacgaaaag tcagtgaaga gaacgttccc
    78481 attacatctg tgtgatgaag atcacatagg gtatgccttc atggcgaaaa aaagtataaa
    78541 attcttaata aacagccggt tatagctccg aaagcgagtt atttattagc gaataaattt
    78601 ccatactgag gaagggatct tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg
    78661 atctgcggct gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc
    78721 gataacacgc tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt
    78781 tttcgttaaa ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata
    78841 aactcgcagg attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact
    78901 tccgccattt ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt
    78961 catccagata ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt
    79021 aattaagtgc gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg
    79081 aggttgggtc gttaacccag ccatgattgc tttcttcaag gtttgcaagg cagtcatgat
    79141 acagggtttc acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat
    79201 ccataacgcg tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac
    79261 ttactgacct ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt
    79321 tttcatcgca ttattacgaa aaattacaat taggaaggaa gcgggacagg actcactttt
    79381 tcatactagc accaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt
    79441 cagaattatc gccagaagaa attggtgaca ctaataccag gattgctctg aatatgacgt
    79501 aataaccgag gaatgaataa agaattaccg caaataatta agaatagcct ctaaatggtt
    79561 atggattatt aaatctatta gcgctactta atataaatta actaacaatc agtatttttt
    79621 atgaatttta tacgtggtta atactggttt tcgtatggga tcctgagttg gtg
//
LOCUS       DAOFSV010000006        71095 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.006, whole genome shotgun sequence.
ACCESSION   DAOFSV010000006 DAOFSV010000000
VERSION     DAOFSV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.006"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..776)
                     /locus_tag="QBH27_000455"
     CDS             complement(<1..776)
                     /locus_tag="QBH27_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7012824.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEH"
     gene            933..1853
                     /locus_tag="QBH27_000456"
     CDS             933..1853
                     /locus_tag="QBH27_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7012825.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFNASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            2026..3168
                     /locus_tag="QBH27_000457"
     CDS             2026..3168
                     /locus_tag="QBH27_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDQ7012826.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNYDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            3641..3742
                     /locus_tag="QBH27_000458"
     CDS             3641..3742
                     /locus_tag="QBH27_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012827.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            4107..4370
                     /locus_tag="QBH27_000459"
     CDS             4107..4370
                     /locus_tag="QBH27_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDQ7012828.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            4370..4510
                     /gene="ykgO"
                     /locus_tag="QBH27_000460"
     CDS             4370..4510
                     /gene="ykgO"
                     /locus_tag="QBH27_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDQ7012829.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(4580..4770)
                     /locus_tag="QBH27_000461"
                     /pseudo
     CDS             complement(4580..4770)
                     /locus_tag="QBH27_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5594..6136
                     /gene="ecpR"
                     /locus_tag="QBH27_000462"
                     /gene_synonym="matA"
     CDS             5594..6136
                     /gene="ecpR"
                     /locus_tag="QBH27_000462"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDQ7012830.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            6211..6798
                     /gene="ecpA"
                     /locus_tag="QBH27_000463"
                     /gene_synonym="matB"
     CDS             6211..6798
                     /gene="ecpA"
                     /locus_tag="QBH27_000463"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDQ7012831.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            6855..7523
                     /gene="ecpB"
                     /locus_tag="QBH27_000464"
                     /gene_synonym="yagY"
     CDS             6855..7523
                     /gene="ecpB"
                     /locus_tag="QBH27_000464"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDQ7012832.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            7549..10074
                     /gene="ecpC"
                     /locus_tag="QBH27_000465"
                     /gene_synonym="yagX"
     CDS             7549..10074
                     /gene="ecpC"
                     /locus_tag="QBH27_000465"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDQ7012833.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            10064..11707
                     /gene="ecpD"
                     /locus_tag="QBH27_000466"
     CDS             10064..11707
                     /gene="ecpD"
                     /locus_tag="QBH27_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDQ7012834.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            11676..12386
                     /gene="ecpE"
                     /locus_tag="QBH27_000467"
     CDS             11676..12386
                     /gene="ecpE"
                     /locus_tag="QBH27_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDQ7012835.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            12693..13022
                     /locus_tag="QBH27_000468"
     CDS             12693..13022
                     /locus_tag="QBH27_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDQ7012836.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(13034..13197)
                     /locus_tag="QBH27_000469"
                     /pseudo
     CDS             complement(13034..13197)
                     /locus_tag="QBH27_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(13271..13885)
                     /locus_tag="QBH27_000470"
     CDS             complement(13271..13885)
                     /locus_tag="QBH27_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDQ7012837.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(14100..14285)
                     /locus_tag="QBH27_000471"
     CDS             complement(14100..14285)
                     /locus_tag="QBH27_000471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012838.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            14334..14492
                     /locus_tag="QBH27_000472"
                     /pseudo
     CDS             14334..14492
                     /locus_tag="QBH27_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            14719..15054
                     /locus_tag="QBH27_000473"
     CDS             14719..15054
                     /locus_tag="QBH27_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDQ7012839.1"
                     /translation="MAILITQDRQVANLTPPEGKSRITVSVKSKVGSGLSVESRSGSQ
                     TKSWIYRPYLNGKQIKITLGSYPAMTLSQAREAHADALALVKQGIDPRYARKTEKQQN
                     EQMPIFSQL"
     gene            15262..16038
                     /locus_tag="QBH27_000474"
     CDS             15262..16038
                     /locus_tag="QBH27_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ7012840.1"
                     /translation="MTLDHAALQGLIDHNPARLLKPAMFGASMGKPRERWLPQDELQM
                     LWKALDEAGFGGGSVATGGRGIASSVILSQAIANALRLIILTGVRRSEAVNMRWEQIN
                     GNRWTIPETKNGKSHIVTLHPLALSLLKTQRIISEGGWVFESLSKPSFPVTGDAITRA
                     LERLKTKYMAEVAPFSPHDLRRSIATGCAEYLDAPERLIELLLNHVPKDRLIRTYQVG
                     QQAEKLRNLFLKWGDFIEHEIIESDNGTPDNVIQISFGKR"
     gene            complement(16203..17123)
                     /locus_tag="QBH27_000475"
     CDS             complement(16203..17123)
                     /locus_tag="QBH27_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDQ7012841.1"
                     /translation="MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAMLKDKNPAKYVAAVNKLERVLE"
     gene            complement(17110..17892)
                     /locus_tag="QBH27_000476"
     CDS             complement(17110..17892)
                     /locus_tag="QBH27_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDQ7012842.1"
                     /translation="MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLTSLALQGHAHYTEPGKPQVWLAL
                     PAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
                     CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYL
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRTFKPEGVEDGSA"
     gene            18176..21274
                     /locus_tag="QBH27_000477"
     CDS             18176..21274
                     /locus_tag="QBH27_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDQ7012843.1"
                     /translation="MKFTEDTRVKIPVILHLIRLGYHYLSLKEQRWDKETNIFPDLFT
                     AAIGRINPGLAPDDIGRLLKDLTLLLDNDDLGRAFFEKLSDRSGIKLIDFDNFNNNSF
                     HVVTELTCENGDEEFRPDITLLINGMPLIFIEVKKPNNREGIVAERNRINERFKNPKF
                     KRFANITQFMIFSNNMEYDDSDPEPIQGAFYASSSYSNPVFNYFREEELFNLTNLLKP
                     LSEETEIAVLKDNNLEVIRTNPEFQTNKDHNRPTNRLCTALLSRDRLAFILRYALVYV
                     AEPGGIQKHIMRYPQLFATKAIERKLNAGVKKGIIWHTQGSGKTALAYYNVRFLTDYF
                     QNQQVIPKFYFIVDRLDLLQQAQREFTARGLIVHLIDSREAFTRDIKATQALHNHHGK
                     AEITVVNIQKFRDDPDVVTTKDYDINIQRVYFLDEVHRSYNPKGSFLANLSQSDTNAI
                     KIGLTGTPLLGTDYNSRDLFGDYIHKYYYNASIADGYTLRLIREEIATNYRMILQQAL
                     KEVEVRMGDVDRKYIYAHPSFVKPMLNYIVTDFAKSRGALNDDTIGGMVICDSSEQAK
                     QMFEIFNTDYAESPTAADEVSEEMPVLQAAEPAAVYHLHAKETRKIKRAALILHDIGS
                     KQERKEWVEQFKAGKIDLLFVYNMLLTGFDAKRLKKLYLGRVIRKHNLLQALTRVNRT
                     YNHFRYGYVVDFADIRQEFDATNKAYFEELQAELGDEMEHYSRLFKSAEEIREEIEHI
                     KDVLFSFDTENAEIFTDQINQIQDRETALALKNALADARSLYNLIRLQGNTEFLQALD
                     FNKLNILYREASRRLDLLNLKADLEQGVDLSGLLNRALEEVIFEFHKVGEEELVLADE
                     LKETLRRTREALAANFDQQDPQFVSLKDELERLFKKKKLSEVTQQEMVANIGTLNKIH
                     GRIKELNRQNNLLRQKYRGDVKYARTHKRLRERGGLSEPERKIFEALLSVKLDADEKV
                     LNNSQILNNESYFERHMESCVITHFEDEHHITLTSSAVSDINRLIVAEYLNEFNTGAR
                     TW"
     gene            21268..22905
                     /locus_tag="QBH27_000478"
     CDS             21268..22905
                     /locus_tag="QBH27_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDQ7012844.1"
                     /translation="MVELEFRDKTKALIDSLKSICANYGLGNDGNEFKIITQAFLYKF
                     LNDKFAFEAKQKDKSIASAESWEDALSAMSEDQLKKLQQRMAPDTARLKPHHFIRYLY
                     NRQNAADFARTFDDTLMDIAATNNDVFAVKTDGGAKVVLFERLSQYIADESKRDDFCR
                     AIINKLADFSFERIFTQKFDFYATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMAEIL
                     VPKAQQGVVRNVSCYDPSAGSGTLLMNVAHAIGEDRCSIFAQDISQKSSSLLRLNLIL
                     NNLVHSIPNVIQGNTILHPFHKDGGALKRFDYIVSNPPFKMDFSDFRDALDSKENQQR
                     FFAGIPKIKAKARDKMEIYQLFLQHIIFSLKPGGKAAVVVPTGFITAQSGIDKGIREH
                     LVQNKMLAGVVSMPSNIFATTGTNVSILFIDASNKEKVVLIDASNLGEKVKDGKNQKT
                     VLTECEEKRICEAFNNKWSEEDFSVVVSYDDIAAKNYSFSAGQYFDVKIEYTDMTPEQ
                     FAAKMKGFTENLNNLFEQSRELEVQIKMQMADIKYEI"
     gene            22895..24142
                     /locus_tag="QBH27_000479"
     CDS             22895..24142
                     /locus_tag="QBH27_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDQ7012845.1"
                     /translation="MKFNYLKIKDVCDFVGGSQPPKSQFIYVSKPGYVRLIQTRDYKT
                     DAFPTYIPISSTKKFCDEFDIMIGRYGPPIFQICRGLKGAYNVALLKVIPKEGVSRDF
                     LYYFLKQDSVFQYVDKLSARTGGQTGVDLVSLKEYPVRIPEEIECQEKLVTILSVIDK
                     KIALNNRINTELEAMAKTLYDYWFVQFDFPDANGKPYKTSGGKMEYNATLKREIPAGW
                     NDSILGKFIELDRGVTYSKEDVRTQDDKDTIGILRATNVTGNNVDIDDLVFIPSSRVN
                     VNQMLNKFDILIVMSSGSKEHVGKNGVYYFEKKHAFGAFCSKITPVRKYRYFINTFLQ
                     SKWFKSYINNQCLGTNINNLTNTHITNCEIICPTPDVVALFENKMMPIYNKLASNTQE
                     NSHLIQLRDWLLPLLMNGQVTVK"
     gene            24322..24507
                     /locus_tag="QBH27_000480"
     CDS             24322..24507
                     /locus_tag="QBH27_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDQ7012846.1"
                     /translation="MCRAFGYTSMAGRAGASQDAPVSCNAGKANPVRFRPHEISLSSG
                     GHNKPLQETPYGYNPHL"
     gene            24485..24955
                     /locus_tag="QBH27_000481"
     CDS             24485..24955
                     /locus_tag="QBH27_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012847.1"
                     /translation="MATILISDCDLLDLLSAPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLIESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKTEQTLTGLLCELVWNFTEGLKSPRWLRTADGVKFISEVAA"
     gene            25101..25304
                     /locus_tag="QBH27_000482"
                     /pseudo
     CDS             25101..25304
                     /locus_tag="QBH27_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(25319..25394)
                     /locus_tag="QBH27_000483"
     tRNA            complement(25319..25394)
                     /locus_tag="QBH27_000483"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25359..25361),aa:Thr,seq:cgt)
     gene            complement(25509..26762)
                     /gene="proA"
                     /locus_tag="QBH27_000484"
     CDS             complement(25509..26762)
                     /gene="proA"
                     /locus_tag="QBH27_000484"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDQ7012848.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(26774..27877)
                     /gene="proB"
                     /locus_tag="QBH27_000485"
     CDS             complement(26774..27877)
                     /gene="proB"
                     /locus_tag="QBH27_000485"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDQ7012849.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            28166..29221
                     /gene="phoE"
                     /locus_tag="QBH27_000486"
     CDS             28166..29221
                     /gene="phoE"
                     /locus_tag="QBH27_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDQ7012850.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(29260..29661)
                     /gene="crl"
                     /locus_tag="QBH27_000487"
     CDS             complement(29260..29661)
                     /gene="crl"
                     /locus_tag="QBH27_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDQ7012851.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(29719..30963)
                     /gene="frsA"
                     /locus_tag="QBH27_000488"
     CDS             complement(29719..30963)
                     /gene="frsA"
                     /locus_tag="QBH27_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDQ7012852.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(31055..31513)
                     /gene="gpt"
                     /locus_tag="QBH27_000489"
     CDS             complement(31055..31513)
                     /gene="gpt"
                     /locus_tag="QBH27_000489"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDQ7012853.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            31774..33231
                     /gene="pepD"
                     /locus_tag="QBH27_000490"
     CDS             31774..33231
                     /gene="pepD"
                     /locus_tag="QBH27_000490"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDQ7012854.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(33287..33901)
                     /locus_tag="QBH27_000491"
     CDS             complement(33287..33901)
                     /locus_tag="QBH27_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDQ7012855.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(33898..35037)
                     /locus_tag="QBH27_000492"
     CDS             complement(33898..35037)
                     /locus_tag="QBH27_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDQ7012856.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(35227..35679)
                     /locus_tag="QBH27_000493"
     CDS             complement(35227..35679)
                     /locus_tag="QBH27_000493"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ7012857.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(35676..36731)
                     /gene="dinB"
                     /locus_tag="QBH27_000494"
     CDS             complement(35676..36731)
                     /gene="dinB"
                     /locus_tag="QBH27_000494"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDQ7012858.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(36802..37725)
                     /gene="lafU"
                     /locus_tag="QBH27_000495"
     CDS             complement(36802..37725)
                     /gene="lafU"
                     /locus_tag="QBH27_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDQ7012859.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAAPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(37728..38591)
                     /gene="motA"
                     /locus_tag="QBH27_000496"
     CDS             complement(37728..38591)
                     /gene="motA"
                     /locus_tag="QBH27_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDQ7012860.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(38604..39320)
                     /locus_tag="QBH27_000497"
     CDS             complement(38604..39320)
                     /locus_tag="QBH27_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDQ7012861.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(39340..39807)
                     /locus_tag="QBH27_000498"
     CDS             complement(39340..39807)
                     /locus_tag="QBH27_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDQ7012862.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(39815..40876)
                     /locus_tag="QBH27_000499"
     CDS             complement(39815..40876)
                     /locus_tag="QBH27_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDQ7012863.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(40873..41184)
                     /locus_tag="QBH27_000500"
     CDS             complement(40873..41184)
                     /locus_tag="QBH27_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDQ7012864.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(41189..41581)
                     /gene="fliS"
                     /locus_tag="QBH27_000501"
     CDS             complement(41189..41581)
                     /gene="fliS"
                     /locus_tag="QBH27_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDQ7012865.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(41604..42920)
                     /gene="fliD"
                     /locus_tag="QBH27_000502"
     CDS             complement(41604..42920)
                     /gene="fliD"
                     /locus_tag="QBH27_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDQ7012866.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(43330..44244)
                     /gene="lafA"
                     /locus_tag="QBH27_000503"
     CDS             complement(43330..44244)
                     /gene="lafA"
                     /locus_tag="QBH27_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HDQ7012867.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            44730..45572
                     /locus_tag="QBH27_000504"
     CDS             44730..45572
                     /locus_tag="QBH27_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDQ7012868.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEALIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(45631..46608)
                     /locus_tag="QBH27_000505"
     CDS             complement(45631..46608)
                     /locus_tag="QBH27_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HDQ7012869.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVASTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRELQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQAVRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQILLQAVNGQARISSQTVRNLLG"
     gene            complement(46625..47554)
                     /gene="flgL"
                     /locus_tag="QBH27_000506"
     CDS             complement(46625..47554)
                     /gene="flgL"
                     /locus_tag="QBH27_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDQ7012870.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLVVRDDLLEAANGTN
                     TADSLASLGQDIESLTDSMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(47569..48945)
                     /gene="flgK"
                     /locus_tag="QBH27_000507"
     CDS             complement(47569..48945)
                     /gene="flgK"
                     /locus_tag="QBH27_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDQ7012871.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(49135..49434)
                     /locus_tag="QBH27_000508"
     CDS             complement(49135..49434)
                     /locus_tag="QBH27_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HDQ7012872.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMLVKQLSPRHK"
     gene            complement(49434..50534)
                     /locus_tag="QBH27_000509"
     CDS             complement(49434..50534)
                     /locus_tag="QBH27_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDQ7012873.1"
                     /translation="MQNWIKAAAIAVSLTLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMTATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(50549..51214)
                     /gene="flgH"
                     /locus_tag="QBH27_000510"
     CDS             complement(50549..51214)
                     /gene="flgH"
                     /locus_tag="QBH27_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDQ7012874.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(51297..52082)
                     /gene="flgG"
                     /locus_tag="QBH27_000511"
     CDS             complement(51297..52082)
                     /gene="flgG"
                     /locus_tag="QBH27_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDQ7012875.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(52161..52898)
                     /locus_tag="QBH27_000512"
     CDS             complement(52161..52898)
                     /locus_tag="QBH27_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HDQ7012876.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(52898..54100)
                     /gene="flgE"
                     /locus_tag="QBH27_000513"
     CDS             complement(52898..54100)
                     /gene="flgE"
                     /locus_tag="QBH27_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HDQ7012877.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(54372..55085)
                     /locus_tag="QBH27_000514"
     CDS             complement(54372..55085)
                     /locus_tag="QBH27_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HDQ7012878.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(55085..55516)
                     /gene="flgC"
                     /locus_tag="QBH27_000515"
     CDS             complement(55085..55516)
                     /gene="flgC"
                     /locus_tag="QBH27_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDQ7012879.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(55519..55854)
                     /gene="flgB"
                     /locus_tag="QBH27_000516"
     CDS             complement(55519..55854)
                     /gene="flgB"
                     /locus_tag="QBH27_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDQ7012880.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            55936..56673
                     /gene="flgA"
                     /locus_tag="QBH27_000517"
     CDS             55936..56673
                     /gene="flgA"
                     /locus_tag="QBH27_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDQ7012881.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGETV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            56755..57033
                     /gene="flgM"
                     /locus_tag="QBH27_000518"
     CDS             56755..57033
                     /gene="flgM"
                     /locus_tag="QBH27_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDQ7012882.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            57046..57474
                     /locus_tag="QBH27_000519"
     CDS             57046..57474
                     /locus_tag="QBH27_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDQ7012883.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRAREDL
                     LAVNDEITQHYEQLHAHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            57602..58534
                     /locus_tag="QBH27_000520"
     CDS             57602..58534
                     /locus_tag="QBH27_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HDQ7012884.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLYVRKVWKNEIRQSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQAILSTLTLPDLTPELYLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQPARQLMTLLG
                     SCAFDWSILPESPRKTSLPLQMPLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQADGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRESENAVLIRQQIQAERSADPLLYAFSLLSC"
     gene            58581..61019
                     /locus_tag="QBH27_000521"
     CDS             58581..61019
                     /locus_tag="QBH27_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ7012885.1"
                     /translation="MQTLIKTLVTHRVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPESFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAVLTDIVSDPLLRNAYLGLHNATHYQAIHHQAIDQVVLINLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALRLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLHTDKWLACYPSLCYQRQGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSTLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSHKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWCAVQGQLAEIRHHYCDAFMDGK
                     AGERAAMVIMEALG"
     gene            61501..61902
                     /locus_tag="QBH27_000522"
     CDS             61501..61902
                     /locus_tag="QBH27_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012886.1"
                     /translation="MLQKIGYMEEDNIIEKKPWRGIPPEYTDRWREIYNNAAPEEGMH
                     LSDACPVCGDTELYRYYSLLKTQPRLLGGIMYKGPGSYWEWCGSCRSYEHMSGYVPIS
                     WVVELSGIDHSKLTAIPDMIDIARHKNHYFS"
     gene            62927..63475
                     /locus_tag="QBH27_000523"
     CDS             62927..63475
                     /locus_tag="QBH27_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012887.1"
                     /translation="MKTYHLSSANRSAEHYIMFSYGVSLRLSNYLFNSKPVARDFFKH
                     LKGTTQRGLTDKHLDNLDYFKGLGAYHIVSKRFVEAMKSYLKEDVTFHAMQVSGRNTS
                     HDLYLLQVNKTLPIIKETSFDLNNDRVFSPPIFKKNHDISFLIAREENVLMNHIVIVS
                     DEFVSLCARHQLKINFRQTLTE"
     gene            63563..63910
                     /locus_tag="QBH27_000524"
     CDS             63563..63910
                     /locus_tag="QBH27_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012888.1"
                     /translation="MKKVYFVHRDKNAIERQSDGVEFCFIPEFNDGRIYFYCHEYDIF
                     WRSIKDAGDYAWCCNFHLKGIIRPATLIEISNSDLISYIDSIKEYEIENSKLININYI
                     HLNYDFLNIHQNT"
     gene            complement(63930..64325)
                     /locus_tag="QBH27_000525"
     CDS             complement(63930..64325)
                     /locus_tag="QBH27_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HDQ7012889.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGEQLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDD
                     FSCVCEVVYFPRTPSVSTTGIIEVIRGHF"
     gene            complement(64328..64762)
                     /gene="fliJ"
                     /locus_tag="QBH27_000526"
     CDS             complement(64328..64762)
                     /gene="fliJ"
                     /locus_tag="QBH27_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDQ7012890.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(64765..66105)
                     /gene="fliI"
                     /locus_tag="QBH27_000527"
     CDS             complement(64765..66105)
                     /gene="fliI"
                     /locus_tag="QBH27_000527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDQ7012891.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTRLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPIAKKAEGQ"
     gene            complement(66098..66808)
                     /locus_tag="QBH27_000528"
     CDS             complement(66098..66808)
                     /locus_tag="QBH27_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HDQ7012892.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPT
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(66812..67822)
                     /locus_tag="QBH27_000529"
     CDS             complement(66812..67822)
                     /locus_tag="QBH27_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDQ7012893.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINHFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            complement(67800..69446)
                     /gene="fliF"
                     /locus_tag="QBH27_000530"
     CDS             complement(67800..69446)
                     /gene="fliF"
                     /locus_tag="QBH27_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDQ7012894.1"
                     /translation="MNAQIKKLTQAFPAFRLHLVDNKRWVLMAGVGLAVAVTAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(69451..69792)
                     /locus_tag="QBH27_000531"
     CDS             complement(69451..69792)
                     /locus_tag="QBH27_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDQ7012895.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPSMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(69807..70793)
                     /locus_tag="QBH27_000532"
     CDS             complement(69807..70793)
                     /locus_tag="QBH27_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDQ7012896.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETRDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
BASE COUNT        17468 a        18795 c        17625 g        17207 t
ORIGIN      
        1 atatgttcag cgattgcaaa atccagatgt tgaatgatac ccgttccatt cagcgccatt
       61 cgtatcatgc tttcgtcatc attagaaata tacttacctg acacctctat ttcgaactct
      121 tcattacccc ccacaacatt cgaaaactcc catgctgaga gtgcaccgct acttttatgc
      181 cgcaaccgaa gacaattatg gttactcaat tcctgaggag tttcaggaat accgtgttct
      241 tttaaataat ctggcgatgc cacagtaacc agtttaataa gcggagaaac aggaatggca
      301 gtcatatgct catccagtcc ttgctcaaga cgtatgccca catcacagcc gtccgcaata
      361 atgttagata aaccgtcatc cataatgagt tctatctttg ttttggggta acgagtaaga
      421 aactcgccaa tatgaggctc aacaagcatc cttgctgcaa cacgggagga atttattctc
      481 aacagtcctg taggttcaag ttgggtatca ccgacatctt tcaacgcatc atcaatttgc
      541 cccaaagccg acaccagcac ttcatacaga tgctgtcctt cctcagtaag cgacatattg
      601 cgggttgtac ggtatatcag cttggtgtta agacgttcct caagagattt taaattctgg
      661 gacaatgcgg cccgcgtaat acccatttca gaggctgctc tggaaaaact gccggcacga
      721 acaatcagcg caaaccaggc aagggaaggc aaaattgaag gctctatttt catcattgta
      781 aaccttaact taacactgtg atagatatta gaccattgat gataaccaat acaagtcata
      841 ctattacgcc gttcgcgata tgaacacgaa tgccagttgc ggataataga aatgatattt
      901 aaatctgttt aggcaaatcc ctggagcttg aaatgaaaac tgtttcaata aaacacactt
      961 actgggatat agctgctgat atctatttcc ctgacgattt taatagtgaa aaaaagtatc
     1021 ctgccattat cagtgcccat ccaattggaa gctgcaaaga acagacatcg ggtagtgtgt
     1081 atggtgccgc cctggcaaaa gctggcttta tcgtcattgc ttttaatgcc agttttcagg
     1141 gaagcagcgg tggagaaccg cgttatctgg aagatccaac aatgcgagta aaagacttca
     1201 gcattgtcgt tgattacctt accaccttac cttacgttga tgccgggcgt attggtgtac
     1261 tgggtatttg tggtggaggt ggatatgcca ttaatgccgc aatgacagaa cgtcggatta
     1321 aagccattgg tactgtcaca ggtgcaaact acgggcgtct tatgcgtgag ggatttacag
     1381 cgtttaatcc tatcggagca ctcgaagcaa tggcgcaaca aagaacagac gaagctaacg
     1441 gcgcaaaact tcgcgtagat gatttgcttc cttcttctcc ggaagcagct cttgaagcgg
     1501 gattaacaga aatagacctt tttgaagcaa cagaatatta tcgttcgcca cgaggctgtg
     1561 cgcctaatgg ggtaaatcgt tcgctatttt cacatcagac tgttgccgtg ggctgggatg
     1621 cttttcatct tgcagaagtc ctgttaactc aaccactaat ggttgtagta ggcgatagag
     1681 tgggcgcttt tggtgcctat cgtgacggct gcgaaattat tggtcgtgca gcctcaaaac
     1741 ataaagagtt ggtcgtggtt gaaggttatt cacattacga cctgtatgat aagccagaac
     1801 ctgtgaaaca ggcgctcgat aaattaattc ctttttacaa aacacatttg taataacctt
     1861 gcagggagat tcattatctc cctgacattt atcctttatt gactacacga caataataaa
     1921 attactcaaa aagaattcta ttaaaggaaa aaagcatcaa aaagtagcaa ctcaacttat
     1981 taagattata cgcattaaaa tcagataaac atttgaggta ttgttatgac aaataaacac
     2041 ccatcattgt tttctccttt tatgctgaca gaaaaaataa agctacgtaa tcgtatcgtt
     2101 atggctccta tgacaacctg gtcagctaat cctgatggaa ccatctctga gcaagaactc
     2161 gaattttata aacgtcgctc acaaaatgtt ggtttagtaa taacaggatg tacctatgtc
     2221 acgccaagcg gcattgggtt tactcatgaa tttgccgcgt atgacgaccg tttcattaac
     2281 agccttgaaa agttagcagc tgcagccaaa agtggcggtg ctccggctat tttgcagatt
     2341 ttccacgccg gtaataaagc catcccggag cttgtgccaa attacgatgt tattagtgcc
     2401 agcgcatcaa gcgtaaaatc tggtgacttt atgaagagag tggtacaaag cagggaaatg
     2461 actgaaaacg aaattcagga aaccattaga gcattcggag atgtcacaaa aagggcgatt
     2521 aaagcaggtt ttgatggcgt tgaacttcat ggtgcgcacg gattccttct gcagaatttc
     2581 ttctcccctc tatttaacca gcgtaatgac cgatggggag gagatcttga aggacgtatg
     2641 cgcttcccgc tggccgtatt acaggaagta aaaaatgtag tctatgaata tgcgacaaag
     2701 ccctttgcta tcggttacag aatatctcct gaagaatctg taacaggggg actgcggata
     2761 gaggatactt ataaactcct tgatcggctc atttcatccg gtatttcgta tatacatacc
     2821 tcgctggtca gtattaacga tagctaccct gttgagtcgc caaatggtcc ccgaactatt
     2881 gaattgattt taaatcatat tgcaggcaga gtgccggtaa tcgccgcagg taaaatacgc
     2941 acaccgtctc aggctcaaga agcaatttcc gctggcctgc cacttgttgc gatcggtaaa
     3001 gggctggtga taaatccaga atgggtgact ttagctgaat caggtagaag tcatgaaatt
     3061 caaacagccc ttaatccaca acgcgttcct gaattaacca ttccggataa actatgggat
     3121 caaattcagg catcgaaagg aactggctgg tttccgttaa tggattaaat atcgattact
     3181 ctcccgagag taaaactatc aaatcaatga gtaaccgata cagcctcgca aattctttca
     3241 gataattaac ccgtcactgc ggcacgttct ctcgcaaggc gtgcctgcag tgtaatccag
     3301 tatgtatccg gcatagttga cggtttaata gactcaattc ctctccctgg ttacctacaa
     3361 cgatatcttg tataacaccc caggagatac gacgttacga tgagatgcga tatgctcctc
     3421 agcgccaatc aacgccagga ctgggccagt cttgagatca gactcattcc cgtaactgat
     3481 ttcaggcgac atatatcaat gacgttgggg caataaaaga tagccgatgt aatacatcta
     3541 ttcaaatttg tgatacatgt caaaaaattg gttgaccaaa ctcgttattt atataagggc
     3601 acttacgaag tgcactcttt tttaaagcga ggaagtacca atgaaagaga ataaagtaca
     3661 gcaaatcagt cataaactga ttaatatcgt tgtttttgtc gcaattgtag aatacgccta
     3721 tttatttctc catttctatt aataacggca ataaactgtt cacttcagtg atatttaaaa
     3781 tatgcatcct ctcccttttt tgtaagtaat tattatatcc gtgggagagg aatacacatt
     3841 gtcaggtaat caatcatgct gcaataaatc atcggccagt aaagtggaga tagcctccat
     3901 tctcgaaaaa tccatactct cagcgaaacc atcatcaatc actcatccag gcgtttatgg
     3961 gagcgtcgcc aatggctgct aacaatgcca gacttccccg ttgcggaaat tccacatccc
     4021 acaaatagtc acagtgattg ggtgttgaaa tgatccggat gagcatgtat ctttacggtt
     4081 atgttataac ataacaggta aaaatgatga aacccaatat ccatcctgag tatcgtactg
     4141 tggtgttcca cgacaccagc gttgatgagt actttaaaat cggctcgact atcaaaacag
     4201 accgtgagat tgagctggat ggcgtaacgt atccgtacgt aacaattgat gtctcttcga
     4261 aatcgcaccc gttctataca gggaagctga gaacagtggc atcagaagga aatgttgcac
     4321 ggttcaccca acgttttggt cgttttgtta gcacgaaaaa gggggcatga tgaaagtcct
     4381 taactctctg cgtaccgcaa aagaacgcca tccagactgt cagattgtga agcgaaaagg
     4441 acggctgtat gtgatttgta aatctaatcc acgttttaag gccgttcagg gtcgtaagaa
     4501 aaaacgttga tttaaaattc gacgaattaa cgatatttgt ctgattaata atcagaccgg
     4561 attaatgttg gtgtatttat tacaccagca ttaattttcc tggggatgta tcttcctgtt
     4621 catttaaggt caactgcctg acgtttctct ccgaatattc cattatatta atgttgactt
     4681 gttgaccagc ttcgccctgt atgctggcat caactctctt ttagactgaa cacgccactc
     4741 agtttcctcc ctttgcgacg cagcctgcat ttacactcaa actgttaaga tgataaatgt
     4801 ggtaaatctg ttggtactga cataaaaacg tttacgccac aggaacggcc agatccatcg
     4861 gtaaccccat cgccgacgtt cgagtgccag ttagagtaac gcgcacagat gactgaatgc
     4921 agtgccctgg caaagaggcc atcgttcctg tgacaactgg cagtcttcgt ttaacttcac
     4981 ttaatttagc tcttaggggg cttaccggac agatgacgta cttacacctg tttaattttc
     5041 catcacttat tggaatgaac acccataacc attttgtgcg gcatgttaat ccattaaaac
     5101 accttactga ttggcaaatc atctttaatt atgacttatg atagttttat attctatttc
     5161 ttatcattta aacttgtttt tttactagtc cattacacaa cacattaaga ctattcctaa
     5221 cacctcaggg caaagttcct ggctaatata aaatgcaagt aagaattgaa cgttatattg
     5281 ccaataacct tatgaaactg aatgtctttt tcttcttatc aaaaaagcaa tattttcatt
     5341 ttttgtaaat attgacttaa ccatggaatt cattttctgt tcacatattg acactcatca
     5401 ggaaaaaaac ataaatttaa acctaatcga aataattaaa acttaatctc gtttaaccta
     5461 tattgatatg tgctacgtat cttatttact tccgatttac taaaaaaact gaatgtacct
     5521 gtaaaaatta caggtttgga aagtagtgac atggcaaaat gattacagca gggactatga
     5581 ggttaaaaac catatggaat gtcaaaaccg ttctgataaa tacatctggt ctccccatga
     5641 cgcctacttc tataaaggac tatctgaact gattgtggat atcgacagat taatttatct
     5701 atcgctggag aaaatcagaa aagatttcgt gtttatcaat ctcaatacgg attctttaac
     5761 tgagtttata aaccgtgata atgagtggtt atccgcggta aaggggaaac aggtcgtatt
     5821 gattgcggcc agaaagtcag aagccttagc aaattattgg tattacaaca gcaatattag
     5881 gggcgtggta tacgctggac tgagtcgtga tattagaaaa gaactggcct atgtgattaa
     5941 tggcaggttc ctgagaaaag atattaagaa agataaaatc actgaccggg aaatggaaat
     6001 tatccgcatg acggctcagg gaatgctgcc taaatcgatt gccagaattg aaaattgtag
     6061 tgtgaagaca gtgtataccc atcggcgtaa tgcagaggcc aagctgtact caaaattata
     6121 taagttggtt cagtaaactc caggcaagtt agttttaaaa aatgactcac cgggacttca
     6181 tgtcctcaat tcaactcggg aagaaaagca atgaaaaaaa aggttctggc aatagctctg
     6241 gtaacggtgt ttaccggtac aggtgtggcg caggctgctg acgtaacagc tcaggctgta
     6301 gcgacctggt cagcaacagc caaaaaagac accaccagta agctggttgt gacgccactc
     6361 ggtagcctgg cgttccagta tgccgaaggc attaaaggtt ttaactcaca aaaaggtcta
     6421 tttgacgtgg ctatcgaggg tgactcaacg gctaccgcct ttaaactgac ctcacgtctt
     6481 atcaccaaca ccttaaccca gttggatacc tcaggttcca cactgaatgt gggcgtggat
     6541 tataacggcg cggcagtcga aaaaactggc gataccgtga tgatcgatac cgccaacggc
     6601 gtactgggcg gcaaccttag cccactggct aacggttaca atgccagcaa tcgtaccact
     6661 gcacaggatg gtttcacttt ctccatcatc agcggcacca ccaatggtac caccgcagta
     6721 accgattaca gcactctacc ggaaggcatc tggagcggcg acgttagcgt acagttcgac
     6781 gctacctgga ccagttaatc tctctgatgt accagcaggg gtacccccct gctttattcc
     6841 tggacggaat tgttatgaaa aagcaccttc tgcctctcgc tctgctgttt tccggaatat
     6901 caccggccca ggcgctggat gtcggcgata tatcatcgtt tatgaacagt gacagcagca
     6961 cgctgagcaa aacgatccaa aacagtaccg acagtggccg cctcatcaat atccgtctcg
     7021 aacggctctc ttcaccgctt gacgacgggc aggttatcgc aatggacaag ccggatgagt
     7081 tgctactcac tcccgccagc ttgctgctac ccgcccaagc cagcgaagtg atccgcttct
     7141 tctataaggg accagcagat gaaaaagagc gctactaccg cattgtctgg tttgatcagg
     7201 ccctcagtga tgcgcagcgc gataatgcca accgcagcgc tgtggccact gcctccgccc
     7261 gcatcggcac catcctggtc gtcgcacccc gccaggcaaa ctaccacttt cagtacgcca
     7321 acggctccct gacaaataca ggaaatgcga cgctgcggat cctcgcctac ggcccctgcc
     7381 tgaaagccgc caatggtaag gagtgtaaag agaattacta cctgatgccg ggcaagtcgc
     7441 gtcgttttac ccgcgtggac acggcggata acaaaggacg ggttgcactt tggcagggtg
     7501 ataagttcat tcccgtgaaa tagatagctg tgcagatgga taacgacaat gcctttacga
     7561 cggttctccc caggactgaa agcccagttt gccttcggca tggtcttttt gttcgttcag
     7621 cccgatgcca gcgctgctga cataagtgcg cagcaaatag gtggggtgat tattccgcag
     7681 gccttcagtc aggcgctcca ggacggcatg agcgtcccgc tttatattca tctcgccggt
     7741 agccagggtc gccaggacga tcagcgaatc ggcagcgctt ttatctggtt ggatgatgga
     7801 cagttacgca tccgaaaaat acagctggaa gagagtgaag ataacgccag tgtcagcgaa
     7861 caaactcgac agcagctgat gactctggcc aacgccccgt tcaatgaggc ccttaccatc
     7921 cccctgactg acaacgcgca gttggatctc agcttgcgcc aactgctgct gcagctggtg
     7981 gtcaagcgcg aagcgctggg caccgtacta cgctcacgta gcgaagacat cgggcagtcc
     8041 agtgttaaca ccctcagcag taatctgagc tataacttcg gcgtctataa caaccagttg
     8101 cgtaacggcg ggagcaacac ctccagctat ctgtcgctga ataacgttac tgcgctgcgc
     8161 gaacatcacg tggtgctcga cggctcactg tacgggattg gtagcggtca acaggacagt
     8221 gaattatata aagcgatgta tgaacgcgat tttgctggtc accgatttgc cggtggaatg
     8281 ctcgacacct ggaacttgca gtccttaggg ccgatgaccg ccatttcagc agggaagatt
     8341 tacggccttt cctggggaaa ccaggccagc tccaccatct tcgacagcag ccagtcggcc
     8401 acgccagtga tcgccttttt accggcggcg ggcgaagtac atctcacccg tgatgggcgg
     8461 ctactaagcg ttcagaactt caccatgggc aatcatgaag tggatacccg gggtctaccg
     8521 tacggtattt acgatgtgga agttgaggta atcgttaacg gtcgcgtgat cagcaaacgc
     8581 acccagcggg tcaataagct gtttagccgg gggcgtggcg tcggtgcacc gctggcgtgg
     8641 cagatatggg gcggtagctt tcatatggat cgctggtcgg aaaacgggaa aaagacgcga
     8701 ccagccaaag agagttggct ggcaggtgcc tcgacctccg gctcactgag tacgtttagc
     8761 tgggcggcaa cgggatatgg atacgataat caggcggtgg gtgaaacccg tctgacgctt
     8821 ccgcttgggg gggcgatcaa cgttaacttg caaaacatgc tggccagtga cagctcatgg
     8881 agcaacatcg ccagcatcag cgccactcta cctggaggct tcagttcgct gtgggttaac
     8941 caggaaaaaa cccgcattgg caatcaattg cgacgtagcg atgccgacaa ccgtgcaatc
     9001 ggcggcacac tcaacctgaa ctcactgtgg tcgaagctgg gtacgttcag catcagctac
     9061 aatgatgacc gccgctacaa cagccactat tacacggcag attactatca aagtgtctac
     9121 agcggtacct ttggttcgct tggcctgcgg gccggtattc agcgctataa caacggcgac
     9181 agcagcgcca atacagggaa atatatcgct ctcgatctct cgctaccact gggcaactgg
     9241 tttagcgcag ggatgaccca tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa
     9301 cagtttgatg aaggaaccat tcgcactgtt ggtgccaatc tgtcacgagc catctccggc
     9361 gataccggtg atgacaaaac cctcagcggt ggggcgtatg cacagttcga cgctcgttac
     9421 gccagcggaa cgctgaacgt caatagcgcg gcggacggct acatcaatac taatttgacc
     9481 gccaacggca gcgtcggctg gcagggtaaa aacatcgctg ccagcgggcg gaccgatggc
     9541 aacgctgggg tgatattcga cactggcctg gagaacgatg gtcagatcag cgccaaaatc
     9601 aacgggcgga ttttcccgct taacggcaag cgtaactatc tcccgctctc tccctatgga
     9661 agatatgagg tggagttgca gaacagcaaa aactcactcg acagttacga tatcgtcagc
     9721 ggtcgcaaaa gtcatctgac tctctatcca ggcaatgtcg ctgtcattga gccagaggtg
     9781 aagcagatgg ttaccgtctc cggtcgtatc cgtgcggaag acggcacact gctggctaac
     9841 gcacggatta acaaccatat cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg
     9901 gacgtggata agaaataccc cactatcgat tttcgctaca gtggcaataa aacctgcgaa
     9961 gtggcactgg aactcaacca ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc
    10021 ggcctctcat cgtgggcggc ggtgacgcag acaggagaag agaatgagag ttaacctact
    10081 gatagcgatg ataatctttg cgctaatctg gcctgcaacc gcgctcagag cggcagtgag
    10141 caaaacaacc tgggcggatg ctccggcacg cgagtttgtg tttgtcgaaa acaactcaga
    10201 cgacaacttt ttcgtcactc ctggcggggc gctggatccg cgcctgaccg gtgccaaccg
    10261 ctggaccggt ttaaaataca atggttcagg aaccatctat cagcaaagcc tcggctacat
    10321 tgataacggt tacaacaccg gcctttatac caactggaag tttgatatgt ggctggaaaa
    10381 ttcaccagtt tcatctcctt taactggctt gcgctgcatc aactggtacg cagggtgtaa
    10441 tatgaccacc agtcttatcc tgccgcaaac caccgacgcc agtggatttt atggcgcgac
    10501 ggtgaccagc ggcggcgcga agtggatgca cggcatgttg tcagacgcgt tttaccagta
    10561 tctgcaacaa atgcccgtcg gcagcagctt tacaatgacc atcaatgcct gccagacctc
    10621 tgtgaactat gacgccagca gcggcgcacg ctgtaaggat caggcctccg gcaactggta
    10681 tgttcgcaac gtcacccata cgaaagcagc aaatctgcgg ttgataaata cccactcgct
    10741 ggcggaagta tttatcaaca gcgacggagt accgactctg ggcgaaggga acgccgactg
    10801 ccggacgcaa accatcggca gccgttcagg attaagttgt aagatggtta actataccct
    10861 gcaaacaaac ggactcagca acacctcaat ccatatattc ccggcgatcg ccaactcgtc
    10921 gttagcctcg gccgtcgggg cgtacgatat gcagttcagt ctgaatggca gttcatggaa
    10981 accggtgagc aatactgcct attactacac cttcaacgag atgaagagcg cagactcgat
    11041 ctatgtgttc ttctcgagca acttctttaa gcagatggtg aacctcggga tcagcgatat
    11101 caacaccaaa gatctgttca actttcgctt tcagaacacc acatcaccgg agtctggctg
    11161 gtatgaattt tctacctcca acacgctgat tatcaaaccc cgtgatttca gcatcagtat
    11221 tatctccgat gaatatactc agacaccgtc gcgggaggga tatgtcggca gcggcgagtc
    11281 ggcactcgat ttcggctata tcgtaaccac cagcggtaaa acagctgccg acgaagtgct
    11341 gatcaaggtg accggacccg cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc
    11401 cgatgacggt aaggcgaaag taccgttccc ggcgacgctt tcctttatta cccgcaacgg
    11461 agctacaaaa acctacgatg ccgggtgcga tgatagctgg cgggatatga ccgatgcgct
    11521 gtggttgacc acaccgtgga ctgatatctc tggcgaagtg gggcagatgg ataagaccac
    11581 agtcaaattt tcgattccaa tggataacgc catttctctg cgtacggtag atgataacgg
    11641 ctggtttggc gaagtcagcg cttcaggaga gattcatgtt caggcgacgt ggcgtaacat
    11701 taactaaggc cctgctgaca gtggtctgta tgctggcggc acctttgaca caggcgattt
    11761 cggtcggcaa tctgactttt tcactgccgt ccgagactga ctttgtcagc aaacgtgtcg
    11821 tgaataacaa caaaagcgca cggatatacc gtattgccat cagtgctatt gatagcccgg
    11881 gcagcagtga attgcgcacc cgaccggtgg atggtgaact acttttcgcc ccccgccagc
    11941 tggcgttgca ggctggtgag agcgagtatt ttaaatttta ctatcatggc ccacgggata
    12001 accgcgagcg ctactaccgg gtctcatttc gcgaggtccc cactcgtaac ctgacaaaac
    12061 gcagcccaag cggcggtgag gtcagcacgg agccggtggt ggtgatggat accattctgg
    12121 tagtacgacc acgtcaggtt cagtttaaat ggtccttcga tcaggtgaca ggaacggtaa
    12181 gtaacaccgg caacacgtgg tttaagctac tgattaaacc aggatgtgat tcgaccgaag
    12241 aggaaggcga tgcctggtat ctacgtccgg gagacgtggt tcatcagcct gagttacgtc
    12301 agccggggaa tcattatctg gtctataacg acaaattcat taagattagc gattcctgtc
    12361 cggctaagcc cccttcggcg gactaagact tatccatcgg acgagggaag aattccacct
    12421 cagagactca aaaatcgttt aaatgatgtg gaagcgatcg tcaatgtcac cgatgagctt
    12481 tctcatatgc acaccacagt gttcaggaga cacggaaagg taccaggcgg cctgctaacg
    12541 agtggtttct tcactaatta ggacgtaaaa tcggtaacgg ctggaaatca ttcaatactc
    12601 acactatcga aagtacacca gccaaccgcg atacgtccct gcatacgacg taccgcagtt
    12661 atctttacga tttatagctg tactggtgaa ttatgagcaa tttgaatcca tgcatgacgt
    12721 gtggtgcctg ttgtgcattt ttccgcgtct ctttttactg ggccgaagcc gacgatgctg
    12781 gcggtaccat tcccgccagg ctcactgaac aaatatcccc ttttcaccga tgcatgagcg
    12841 gtactaatca gaaaaatccc cgatgtattg cccttgcagg aacaccgggc aaaaatgcct
    12901 gctgcacgat atatgaaaat cgatcgtcca catgcagaga attcgccatg tctggtgaga
    12961 acggagaagt caatgaggct tgcaatcgtg caagagctaa atacgggcta acaccgttat
    13021 aaatatacaa taattaattg cattgccccg ccagctgtaa tagcggggct tcgtttttca
    13081 gggacaacaa aacctgaaac ttcttccatt ttccagcaac aatgcctctt cttctgtagc
    13141 gtcctccgga tctacatcat cctgaagtag cgcgatatga tcactgactg tccgcatcag
    13201 aatatgaaaa gcaacgtttt taacctataa aaatggcgct atatttgcgc catttttatc
    13261 attcaaagca ttatctattt gagcctaaag ggatctcagg atctggctca tgggtaattc
    13321 tgtttcgtaa atctctgcga ataatttcaa tagaccagaa ccagactaaa tgtccaaaaa
    13381 tttcagagac attctcatac cacggaagat caaagagagg tggcgtaagt cccatgagag
    13441 ggaatgaaat catatgaaca aaaagttgcg ctaaagcacc tgccagtaag ccctgccaaa
    13501 gtttaatttt cggaaatact tcggcgacca cacagtaacc aacggcaaaa actatcgaaa
    13561 agattatgtg cgttacacca acccagttaa agacatgccc ggcgaaggta taaacagccg
    13621 aatttggatc tgtcagtccc aaccaatccc gaagaaaaat gtacggagga ttgagaaagt
    13681 tacgggagca atcaatttgg cctgcagccc tgattaatga ttccggacca cacgctgcat
    13741 taaacatatc aaccggacta cgtggtggca atggaacttc tgccccccac ttaacaaatg
    13801 cggaaacaac gccagcaatc agcccaatga atgcagcaag accataacgt ctgcggtttg
    13861 gtggagtttg ttcaaatata ttcatatcta ccctgcttgt accattatgt tatacacctc
    13921 tttaggagta ttcataaaac aaggcaaatg taaagaactg tattgttttg tataaccaca
    13981 tggttaccta atcgccaatt aatataagct ccattatttc tccatatttt tatgttagaa
    14041 gtgacaaaga gaaatgtaat gtttctctca gagaatataa atcattcctt ttgcccacct
    14101 taatggtggg cttttttatg caaaacctga acgtcctgtg gtattcctct ggcataatga
    14161 ttttcgcgat taaccaacaa ccagaccagc aaagacgcca ccacaagcac agtaacatcc
    14221 atcaggcttg ccacacccca tgcgtgcggc tcaaagccgc catggcatat taatccgctt
    14281 gccattttcg aacagctgat tggtttggtc cggtgtttcc ctgcttgcac ttaaaaatga
    14341 gcggatagga aactctcgaa atggttcgtc gatatgctca cctcgcacct aatcacctta
    14401 ccgaacacgc acggtaaata gattcgatcc tgaacccatc ggtcccaaat ctgtcccaat
    14461 caaaaaataa ggaaggtatt aatgatgtgt aacttattga tttaagtggt gccgataata
    14521 ggagtcgaac ctacgacctt cgcattacga aaccaaaaag catctatttt tccgcatatc
    14581 tacacgtctc tataaatctc ataaaaatat taaaaatcat ataattaaac aaaaaaccca
    14641 tcttatattg ttccatcgaa tttcgctcta tacaatgcta aacaagtacg ccagcaagta
    14701 cgcgagatga ggccaaaaat ggcaatattg ataacccagg acaggcaagt cgcaaacctg
    14761 actccccctg aaggaaaaag tcggataacc gtcagcgtca aatcaaaagt aggttccggc
    14821 ctgtctgtcg aatcccgttc cgggagtcaa acgaagagct ggatttaccg cccttacctc
    14881 aacggaaaac agattaaaat aacgttaggc tcctaccctg caatgacatt gtctcaggcg
    14941 cgtgaagcac atgcagacgc gctggcgctg gttaaacaag gtattgatcc gcgttatgcg
    15001 cgaaaaacgg aaaaacagca aaatgaacaa atgccgattt tttcacaatt atgaaaaaac
    15061 tggctggcat tccgtgaaac caataaaccc attggggcgc gtactctggc tgattatgag
    15121 ggaacatatc gccgccatct ggagaaaggt ttaggttcag tacgcgtctg cgacctgtcc
    15181 agagcctaca tttatgaaca tctcatacgg atcagaaaga ccagcgccga aggtgtccgc
    15241 aagggactta ttcttctcaa tatgacgctt gaccatgccg ctttgcaggg attgatcgat
    15301 cacaaccctg ctcgcttgct taaacccgca atgtttggtg cctcaatggg aaaaccgcgt
    15361 gagcgttggt tgccacaaga cgagttgcaa atgttatgga aggcactgga tgaagcgggg
    15421 ttcggcggcg gatctgtcgc aaccggaggg cgaggcatcg cgtccagcgt tattctttca
    15481 caagcgattg ctaacgcatt acgcctcatc atcctgacgg gggtgcgacg ttccgaagcg
    15541 gtgaatatgc gttgggagca gattaatggc aaccgctgga caatacctga aaccaaaaac
    15601 ggtaaaagtc atatcgtgac gctacatcct ctggcgcttt ctctattgaa gactcagcgc
    15661 attatttcag aaggcggctg ggtattcgag tcgctcagta agccaagttt tcctgtcacc
    15721 ggtgacgcaa taactcgtgc tcttgaacgc ctgaaaacaa aatatatggc agaagtcgct
    15781 cctttctcac cacatgattt acgccgcagc attgctacag gctgcgctga atatctggac
    15841 gcaccagaac ggcttataga acttctgtta aatcatgtgc ccaaagacag gttaatcaga
    15901 acctaccagg tcgggcagca agcggaaaaa ttacggaatc tgtttctgaa atggggggat
    15961 tttattgagc atgagatcat tgagtctgat aatggtactc cagataatgt tattcagatc
    16021 agctttggaa agagatagtt tcgtattttc atgagtagag atcgtctgat aactcagcga
    16081 tctcttactc gatggtgcta tgtattgaat taaatgaata tgtttgagtt attgccacga
    16141 agttattcat ataaccaaag catcatggcc caattcagag tccctgaaca gcgctaagaa
    16201 tattactcca gcaccctctc tagcttattc accgccgcca catacttcgc cggattttta
    16261 tctttaagca tcgccagatt gcgttgcttc cagcgaatag ccggaagatg ctgaatttca
    16321 ggatagcgat accgcgacca gtcagggctg ttttgcttaa aactgagcaa gaactcgcga
    16381 tccagatccg taaaatgtga cttcaacgcc tgcggcagca aagtcgttac tgacagaagc
    16441 gattccagcg acgtttcctg ctgagtcatc cccgaaaatt cctgagcgta taacgtggca
    16501 atccgatctg tatcccagtt tgaagccagc aactcggcaa tgggacgggg atggcctgcc
    16561 agataacaaa gaaaaccgtc aaaaatttcg cgcgtcaggc ccggtctccc cagcatattc
    16621 aacacatcaa acagatcgcg cggatgctga cgatcaagcg cagcacatat tttaccgcca
    16681 taaagatccg ccagcgagac gacactcatg gtggtgaagc catactccat ttccacacgt
    16741 tcgcacacag gcatttctgc cggaggcaac aacagcccgc gccagaccgg gctaacctcg
    16801 atcttaatct ttgcatcagc ggtattcaca atgatgcgct tttcatccgc tttattttcc
    16861 tgccgaatcg cggtgatccc cggtctgccg ttcagcgatg cagtaattct cttcaaagcg
    16921 tcgttgatcg ccgccagatc ggtatcgcga tccgcaaagc tcttatacgc cagatcgata
    16981 tccaccgata gacgggggaa atcctgcaca aataaattga ttgctgtgcc gcctttcaac
    17041 gcgaaacagg attccttcgc cacatgcggc agagcggcca tcaacagttt aacctgcctg
    17101 gcatagctat cacgcagatc catcttcgac tccttccggt ttaaatgtgc ggggaacagt
    17161 aatttgataa tccggctcca gccagccacc aggtatgatt tgccgctttc ccgtacctgt
    17221 ttctataccg ctttgatcaa gatactgaaa ataagggtgt ccattacgcc gggcaaggta
    17281 aagcatcacc cggttggttt taatcgaatg actggcgcga agcagtgcct gcaatttgcg
    17341 tgggctgaga agattccccc cctgaaacag agcatcggca tgttcgaacg agatcaacga
    17401 aggaacgccg caggcgatct catacgcggc cagctccggt gcactgcctg ctaactgccg
    17461 gtcgccaatt ttcagttccg tagtaaatga cgagacatca acggtaaggc ccgatgtata
    17521 cagcgtgata aatgttgctg attgttcgaa ggcagcaaac catcccggca gtttgacgta
    17581 agccggaagc gcgagccaga cctgcggctt acccggttcg gtatagtgcg catggccctg
    17641 aagcgcaagg ctggttagcc ccgccacccg aacaggcttt tcccactgtg tttgaaggca
    17701 gtaaaccgcg tctacccaat ccggctcacg gcccgtccgg cagtacacac cgtgagcgat
    17761 gcgcctgagc cagccattct gaacatagag ataagaaagc ttacgatcta cgccattgcg
    17821 ggtaagccat gactggagca gcacctgacc gggtagcgtg ttttgcatca accagtttag
    17881 atagtttggc aaatgtcgct cctgaataga cattagtaga aatatttaaa ccagtgtagg
    17941 ttcagggata cgccacagca agaaatagtt taatgattcc tcatttcgtc cacttaaaat
    18001 cgacattgag caaaaaatac aaacttacct acattcaggc aaagtgcgac aaatagactc
    18061 ggtcggttta aagagaacgt gtaacataga tgcaaatcga acagacattg ttgccagccg
    18121 ttccaaggtt gtggggcgct gcatggcctg agacgtgata aatacaggga acacaatgaa
    18181 attcactgaa gatacccgag taaaaatccc cgtcatcctc cacctgatac ggctgggcta
    18241 ccactacctg tcccttaaag aacaacgctg ggacaaagaa accaatatct tccctgacct
    18301 cttcaccgct gcgattggca gaataaatcc cggacttgcg ccagacgaca tcggacgcct
    18361 gttaaaagac ctcacgctgc ttctcgacaa cgatgatctg ggccgcgcct tcttcgaaaa
    18421 actcagtgac cgttccggta tcaaactgat cgactttgat aacttcaaca acaacagttt
    18481 ccatgtcgtc accgaactga catgcgaaaa cggtgatgaa gaattccgcc cggacatcac
    18541 gctgctgatc aacggcatgc cgctaatctt tatcgaagtg aaaaaaccca acaatcggga
    18601 aggcatcgtc gcagaacgca accgcatcaa cgaacgcttt aaaaacccca aattcaaacg
    18661 cttcgccaac atcacccagt tcatgatctt ctccaataac atggagtatg acgacagcga
    18721 cccggagccg attcaagggg cattctacgc cagcagctct tacagcaacc cggtattcaa
    18781 ctacttccgc gaagaagaac tcttcaacct gaccaactta ctgaaaccgc tgtcagaaga
    18841 aaccgaaatc gccgtcctga aagacaacaa cctggaagtg atccgcacca acccggaatt
    18901 tcagaccaac aaagatcaca accgcccgac aaaccgcctc tgcaccgcgc tgttgagccg
    18961 ggatcggctg gcatttatcc tgcgctatgc gctggtgtat gtagccgaac cgggcggcat
    19021 ccaaaaacac atcatgcgct acccgcagct cttcgccacc aaagcgattg agcgcaagct
    19081 gaacgcgggt gtgaaaaaag gcattatctg gcacacccag ggcagtggga aaaccgcatt
    19141 ggcatactac aacgtgcgct tcctgacaga ttacttccag aatcagcagg tgatcccgaa
    19201 attctacttt attgtcgatc gtcttgatct gctccagcag gcgcagcgtg aattcaccgc
    19261 ccgtggctta atcgttcacc ttatcgactc ccgcgaagcc ttcactcgcg atatcaaagc
    19321 aacgcaggcg ctgcacaacc accacggcaa agcggaaatc accgtcgtca acatccagaa
    19381 attccgcgat gacccggatg tcgtcaccac caaagactac gacatcaaca tccaacgcgt
    19441 gtacttcctc gatgaagtcc accgcagcta caaccccaaa gggagcttcc tggcgaattt
    19501 aagccagtcg gacaccaacg ccatcaaaat tggcctgacc ggtacgcctc tgctgggcac
    19561 agactacaac tcccgcgacc tcttcggcga ctacatccac aaatactact acaacgcctc
    19621 catcgccgac ggctacacgc tgcgcctgat ccgcgaagag atcgccacca actaccgaat
    19681 gatcctgcaa caggcgctaa aagaggtgga agtcagaatg ggcgatgtgg atcgcaaata
    19741 catctacgcc catcccagct ttgttaaacc gatgctgaac tacatcgtca ccgactttgc
    19801 caaaagccgg ggcgcgctga atgatgacac catcggcggc atggtgattt gcgacagctc
    19861 tgaacaggcc aagcagatgt ttgagatatt caacactgac tacgccgaat cgcccaccgc
    19921 cgccgatgaa gtctccgaag aaatgccggt attacaggcc gcagaacctg ccgccgttta
    19981 ccacctccac gccaaagaaa cgcggaaaat caaacgcgcc gccctgatct tgcacgacat
    20041 cggcagcaag caggagcgca aagaatgggt ggagcaattc aaagcgggca agattgacct
    20101 gctgttcgtt tacaacatgt tgctcaccgg cttcgacgcc aaacggctga aaaaactcta
    20161 cctcgggcgg gtgatcagaa agcacaacct gctccaggcg ctgacccgcg tcaaccgtac
    20221 gtacaaccac ttccgctacg gctacgtggt ggacttcgcc gacatccgcc aggaatttga
    20281 cgctaccaac aaagcctact tcgaagagct acaggctgaa cttggcgacg aaatggagca
    20341 ctactcccgc ctgttcaaat cggcggaaga gatccgcgaa gaaatcgaac acatcaaaga
    20401 cgttctgttt agcttcgata ccgaaaacgc ggaaatcttc actgaccaga taaaccagat
    20461 ccaggaccgg gaaaccgcgc tggcgctaaa aaacgcgctg gccgacgccc gcagcctgta
    20521 caacctcatc cgcctccagg gcaacactga gtttctccag gcgctggatt tcaacaaact
    20581 caacatcctt tatcgcgaag ccagccgccg cctcgactta ctcaacctaa aagcggatct
    20641 ggaacagggc gttgacctct ccggcctgct gaaccgggcg ctggaagagg tgatctttga
    20701 attccataaa gtgggtgaag aagagctggt actggccgac gagctgaaag agacgctgcg
    20761 ccgcacccgc gaagcgctgg ccgccaactt cgaccagcag gacccgcagt tcgtcagcct
    20821 gaaagacgaa ctggagcgtc tgtttaagaa aaagaaactc agcgaagtca cccagcaaga
    20881 aatggttgcg aatatcggca cactcaataa aattcatggc cgcatcaaag agctaaaccg
    20941 ccaaaacaac ctgctgcgcc agaaataccg gggcgacgtg aaatacgcgc gtacccacaa
    21001 acgcctgcgc gagcgcggcg gcctgagcga gccggagcgt aaaatcttcg aagcactgct
    21061 tagcgttaag ctggatgcag atgaaaaagt gctgaacaac agccagatac tgaacaatga
    21121 aagctatttt gagcggcata tggagtcatg cgtcatcacg cactttgagg atgagcacca
    21181 tatcactctg acctccagtg cggtaagcga catcaaccgc ctgattgtgg cggaatacct
    21241 gaacgaattt aataccggag cacgaacgtg gtagaactgg aatttagaga caaaaccaaa
    21301 gccctgatcg acagcctgaa aagcatctgt gcgaactacg gcctgggcaa cgacggtaac
    21361 gaattcaaaa ttatcactca ggcattcctg tacaaattcc tgaacgacaa attcgctttt
    21421 gaggccaaac agaaagataa aagtatcgcc agcgccgaaa gctgggaaga tgcgctcagc
    21481 gcaatgagcg aagatcaact gaaaaagctg caacagcgga tggccccgga taccgcccgc
    21541 ctgaagccgc accattttat tcgttacttg tataaccgac aaaacgcagc ggactttgcc
    21601 agaacttttg acgacaccct gatggacatc gccgccacca acaacgacgt cttcgcggtc
    21661 aaaaccgacg gcggcgcgaa agtggtgctg tttgagcgcc tcagccagta catcgccgac
    21721 gaatcaaagc gcgatgattt ctgccgcgcc atcatcaaca agctggccga tttcagcttc
    21781 gaacgcatct tcacgcagaa attcgacttc tacgccacca tctttgaata cctgatcaaa
    21841 gactacaaca gcaactccgg cggcaaatac gcggaatact acacgcccca cgccgtggcg
    21901 cgcatcatgg cggaaatcct ggtgccgaaa gcgcagcagg gcgtggtgcg caacgtaagc
    21961 tgttacgacc cgtccgccgg ttccggcacg ctgctgatga acgtggcgca cgccattggg
    22021 gaagatcgtt gcagcatctt tgcccaggat atctcgcaga aatcctccag cctgctgcgt
    22081 ctgaacctga tcctcaacaa cctggtgcat tcgatcccta acgttatcca gggcaacact
    22141 attctgcatc cgttccacaa ggacggcggc gcgctgaaac gctttgacta catcgtctcc
    22201 aacccgccgt ttaagatgga tttcagcgac tttcgtgacg cgctggacag caaggaaaac
    22261 cagcaacgtt tctttgccgg gataccgaaa atcaaagcca aagccaggga caaaatggag
    22321 atttaccagt tgttcctgca acacatcatt ttctcgctga aaccgggtgg caaggcggcg
    22381 gtcgtcgtgc cgaccgggtt tatcaccgcg caatcgggca ttgataaagg cattcgcgaa
    22441 catctggtgc aaaacaagat gctggcgggc gtggtttcca tgccgtcgaa tatcttcgcc
    22501 accaccggta ccaacgtgtc gatcctgttt attgacgcca gcaacaaaga gaaagtggtg
    22561 ctgatcgacg cctcgaattt aggtgaaaag gtcaaagacg gcaaaaacca gaaaacggtg
    22621 ctgacggagt gcgaagaaaa acggatctgc gaagcgttta acaacaagtg gagcgaagag
    22681 gatttctcgg tggtggtcag ctatgacgac atcgccgcga aaaattactc gttcagcgcc
    22741 ggacagtatt ttgatgtgaa aatcgaatac accgacatga cgccggaaca gtttgccgcg
    22801 aagatgaaag gatttacgga gaatctgaat aatctctttg aacagtcgcg cgagttagaa
    22861 gtgcaaataa aaatgcagat ggcagatatc aaatatgaaa tttaattatc tgaaaattaa
    22921 agatgtatgt gattttgtcg gaggaagtca acctccaaaa tctcaattta tttatgtatc
    22981 taaacctgga tatgtcaggt tgatccagac gcgtgattac aaaacggatg catttcccac
    23041 gtatatacca atatcgtcga cgaagaaatt ttgtgatgaa ttcgatatta tgattggtag
    23101 gtatggtccc ccaatttttc aaatatgtcg cggattgaaa ggagcatata acgttgcctt
    23161 gctgaaggtt atacctaaag aaggtgtaag tcgtgatttt ttatactatt ttttgaagca
    23221 agactctgtt ttccaatatg ttgataaatt atctgccaga accggaggac aaactggagt
    23281 tgatctagtt tctttaaaag aatatccagt caggatacct gaagaaattg aatgtcaaga
    23341 aaaattggtg actattctta gtgttattga taaaaaaatc gccctcaaca accgcatcaa
    23401 cacggaactg gaagcgatgg cgaaaacact gtatgactac tggtttgtac agtttgattt
    23461 ccctgatgcc aacggaaaac cgtataaaac ctccggcggg aagatggagt ataacgccac
    23521 gctgaaacgg gaaattccgg caggttggaa tgactccatt cttggtaagt ttattgagtt
    23581 agatcgtggc gttacttata gcaaagagga tgttcgaact caggatgata aagatacgat
    23641 aggcattctt cgcgctacaa atgtgacggg aaataatgtt gatattgatg atttagtctt
    23701 cattccttct agtagagtaa acgttaatca aatgctaaat aaatttgaca tattgatcgt
    23761 gatgtcaagt ggtagtaagg agcatgtagg gaagaatggc gtgtattatt tcgaaaaaaa
    23821 acatgcattt ggcgcatttt gttcaaaaat tacacccgtc agaaaataca gatattttat
    23881 aaatacattc ctgcaatcga aatggtttaa gtcttatata aacaatcagt gcttgggaac
    23941 taatattaat aacctgacca atacgcatat cactaattgt gaaatcatat gccccactcc
    24001 tgatgttgtg gcgttattcg agaataaaat gatgccaatt tataataaac tggcatcaaa
    24061 cactcaagag aatagtcatt taatccaact ccgcgactgg ctcctccctc tacttatgaa
    24121 cggacaggtc acggtcaaat aatccccggc gtggttttgc gaggcgcatt ccagatccgc
    24181 attgcgccgc actcaccgca gcaaaaccgc ttgccgttca taaccgatcg cagtatcttt
    24241 ctcccgcgcc tgcaaaatca ggcgtcggga ttagcctcct gaaaaccgtt acaggcgaca
    24301 cttgacgcac cagcgtcttt tttgtgtcgt gcgttcggct acacctcaat ggcgggccgg
    24361 gcgggggcgt cgcaagacgc gccggtttcc tgtaacgccg gtaaggctaa ccccgtccgg
    24421 ttccgccccc atgagattag cctctctagt ggcggacata ataaaccgtt acaggagacg
    24481 ccctatggct acaatcctca tctctgactg cgatttactc gatctattgt ccgctccctt
    24541 caatgccacc acggatttca ccgtcctggc cgactactgc gcccgcttcg ccgaaaccct
    24601 gatagaaacc gacgatccgg cgctgcggct ggcgctctgc ggacggcttc acgcggctct
    24661 tgcgctattg caacccacgc tgcacgaccc catcccgccg catctgattg aaagcctgac
    24721 cgttgatacc cttcccgcca caccagagca ctttatttcc gaaccagatt ccttgtgcga
    24781 atactgcctc gcgctgctcc agctactgct ggaacgtcca gcagagccaa aaacggaaca
    24841 aaccctgacc gggcttctgt gtgagctggt ctggaatttc acggaaggac tcaaatcccc
    24901 gcgctggctg cgtaccgccg acggcgtaaa atttatcagt gaggtggccg catgatgcaa
    24961 caggactgac attccgccga cattattgcg gtgctgaaaa agcggggaac gcctctggcg
    25021 gcacagagaa aaactcattg cggaagcgct gtaaatatcg ccagaaatca tctggtcctc
    25081 aaggtacttt gatagcaaag gcaagctggc tggttcagtc cggagtctct cttcttgcac
    25141 ttaaagagat gggaggatgg gaaactcttg aaatggttcg tcgttaggct cacctttcag
    25201 ccaggcattt caccgggcac gcgagcaaaa tcgatgcgat tataagtcgc aatggcacaa
    25261 atacggcaca agaggagaac gtggtgtatt taaatatggg gtaactactt gattttaatg
    25321 gtgccgataa taggagtcga acctacgacc ttcgcattac gaatgcgctg ctctaccaac
    25381 tgagctatat cggccctgaa aggacatgtt cacgaacgtg aatcacggtg gacaaggtta
    25441 aaactaaccg ggcgatgcgt caatggcctt gtgaatcaaa tggctacttt tgcatcaccc
    25501 ggttttattt acgcacgaat ggtgtaatca ccaatgccga tccacttgta agtggtcagt
    25561 gcttccagcc ccattgggcc gcgcgcgtgg agtttttgtg tgcttaccgc cacttccgcc
    25621 cccagtccaa actggccgcc gtcggtaaaa cgcgtagagg cgttaacgta aacagcggag
    25681 gaatccactt cgttaacaaa acgctgggcg ttgcgcatat cgcgggtcag gatcgcatcg
    25741 gagtgttgcg tgccgtgttc acgaatatgg gcaatggcgt catccagatc gctaacgatt
    25801 ttgacgttca aatctaatga cagaaactca tcgtcatact cttcggcttt aacagccacc
    25861 accttcgcgg ggcctgcctg caactgcgcc agcgcggctg tatcagcgtg taacgtcacg
    25921 ccgctttccg ccatttgttt gcttaatgcg ggcaggaagc tatcagcgat gtttttattc
    25981 accagcaacg tttctaccgt attacacgtg ctcggacgct gagttttcgc gttgacaatt
    26041 actttcaggg cttcagcgat ctctgcgctt tcatcaacgt aaatatggca tacgcctata
    26101 ccgcctgtga tcaccgggat tgtcgactgc tcgcggcaca gtttatgcaa accagccccg
    26161 ccgcgcggga tcagcatgtc gatgtattta tccatacgca gcatttcact gaccagcgca
    26221 cggtcaggat tatcaatcgc ctgcacggca cccgccggta agccacaaga gttcagggcg
    26281 tcctgaatca ccgccaccgt tgcagcgtta gtgcgacacg tttctttgcc gccgcgcagg
    26341 atcactgcgt tacctgtttt caggcacagc gaagcgacat caaccgtcac gttcgggcgc
    26401 gcttcataaa tcacgccaat aacccccagc ggtacgcgac gacgctcaag acgcaggccg
    26461 ctgtccagta cgccgccatc gattacctgc cccaccggat cggcgaggtt gcacacctgg
    26521 cgcacatcat cggcaatgcc tttcagccgt gcgggcgtca gtgtcagacg gtcaagcatc
    26581 gcttcgctaa ggccattggc acgcgcgtct gcaacatcct gcgcgttagc gttgaggata
    26641 ctttcgcttt gtgcttccag ttcatcggcg attttttcca gcacacgatt tttttcgcgg
    26701 ctggagagtt gcgctaattt atacgaggct tgcttcgcgg caatgcccat ttgttccagc
    26761 atcggccagc tccttaacgg gtaatcatgt catcacggtg aacggcaacc gggccgtatt
    26821 catatcccag tattgcatca atttcttgcg agtggtgtcc ggcaatacgg cgtaatgcat
    26881 cgctgttgta acgactgacg ccgtgggcga tatcgcgacc ttcgaggttg caaatacgga
    26941 tgacttcacc acgcgagaaa ttgccagtca cgcttttaat gcctttcggc aacagggagc
    27001 tgccgcgttc cagaatggcg gcagttgccc cttcatctac cgtgatttca cccgcaggcg
    27061 gcgcaccgaa aatccagcgt ttacggtttt caagcggagt cgcctgggca tggaacagcg
    27121 taccgacgga aatgccttcc atcacatcac caataacgcc cggcttgctg cccgcggcaa
    27181 taatggtgtc gatacccgca cggcaagcca cgtcagcggc ctgcaatttg gtgctcatac
    27241 cgccagttcc gaggcctgaa acgctatcac cggcaatcgc gcgcagtgcg tcatcaatgc
    27301 cgtacacatc tttaatcagt tctgcctgcg gattgctgcg cgggtcagcg gtatacaaac
    27361 ctttttgatc ggtcagcagc aacagtttat cggcacccgc cagaatcgcc gccagcgcag
    27421 aaaggttatc gttatcaccg accttaatct ctgccgtagc gacagcatcg ttctcattaa
    27481 ttaccggaac gatattgtta tcgagcaacg cacgcagggt atcgcgggcg ttcaggaagc
    27541 gttcacgatc ttccatatca gcacgggtca gcaacatctg cccgacatga atgccgtaaa
    27601 ttgaaaacag ctgttcccac agttgaatca gtcgactctg ccctaccgcc gccagcagct
    27661 gttttgaagc gatagtcgct ggcaattccg ggtaccccag gtgctcacgt ccggcggcaa
    27721 tcgcacccga cgtcacaata acaatccgat gcccggcggc atgtaactgc gcgcactggc
    27781 gaacaagttc aacgatatgg gcacggttca ggcggcgcga tccgcctgtt agcacactgg
    27841 tgccgagttt taccaccagc gtctggctgt cactcatgat tctctgccat tcaattttag
    27901 gaaaaaatga tatcaaacga acgttttagc aggactgtcg tcggttgcca accatctgcg
    27961 agcaaagcat ggcgttttgt tgcgcgggat cagcaagcgt agcggcagtt gtttacgctt
    28021 ttattacaga tttaataaat taccacattt taagaatatt attaatctgt aatatatctt
    28081 taacaatctc aggttaaaaa ctttcctgtt ttcaacggga ctctcccgct gaatattcgc
    28141 gcgttaatta aaatcaggaa tgaaaatgaa aaagagcact ctggcattag tggtgatggg
    28201 cattgtggca tctgtatccg tacaggccgc agaaatatat aacaaagacg gtaataaact
    28261 ggatgtctat ggcaaagtta aagccatgca ttatatgagt gataatgaca gtaaagatgg
    28321 cgaccagagt tatatccgtt ttggttttaa aggcgaaaca caaattaacg atcaactgac
    28381 tggttatggt cgttgggaag ctgagtttgc cggtaataaa gcggagagtg atactgcaca
    28441 gcaaaaaacg cgtctcgctt ttgccgggtt gaaatataaa gatttgggtt ctttcgatta
    28501 tggtcgtaac ctgggcgcgt tgtatgacgt ggaagcctgg accgatatgt tcccggaatt
    28561 tggtggcgac tcctcggcgc agaccgacaa ctttatgacc aaacgcgcca gcggtctggc
    28621 gacgtatcgg aacaccgact tcttcggcgt tatcgatggc ctgaacttaa ccctgcaata
    28681 tcaagggaaa aacgaaaacc gcgacgttaa aaagcaaaac ggcgatggct tcggcacgtc
    28741 attgacatat gactttggtg gcagcgattt cgccatcagt ggagcctata ccaactctga
    28801 tcgcaccaac gagcagaacc tgcaaagccg tggcacaggc aagcgtgcag aagcatgggc
    28861 aacaggtctg aaatacgatg ccaataatat ttatctggca actttctatt ctgaaacacg
    28921 taaaatgacg ccaatctctg gcggctttgc caataagacg cagaactttg aagcggtcgc
    28981 tcaataccag tttgactttg gtctgcgtcc atcgctgggt tatgtcttat cgaaagggaa
    29041 agatattgag ggcatcggtg atgaagatct ggtcaattat gtcgatgttg gtgctacgta
    29101 ttatttcaac aaaaatatgt cagcgtttgt tgattataaa atcaaccaac tggatagcga
    29161 taacaaattg aatattaata atgatgatat tgtcgcggtt ggcatgacct atcagtttta
    29221 atggatattg ccggatgtga tgcatccggc acatttcatt cacgccgtta acttcaccgg
    29281 ttcatcacga aaatcatctg ccggttccag cttcagattc agcgtcgcca gcagctcacg
    29341 cagcttctcg tgaaattcac gcagggtgtg ctccagtcgt tcaaccactt cggcatcttt
    29401 tatctgaaca ctcgtccagt cgcctgcttt atcgaacaga ccaaactggt aactgtaggt
    29461 aaaacgggat tcctgcgctt ccagttccat ccaccagccc cagaactcac gcacttccgg
    29521 tgccggtttc acgttgacgc atacagccag acaatcgaaa aagaatcgat tatcttcgca
    29581 cttaccttca cgaatatacg ggcctagtgc ggtaaatttt ttgactaatc tgctcttcgg
    29641 gtgtccactc ggtaacgtca ttgcaatctc ctgttgtgat gcaactgttt taccaaattg
    29701 gtaaaattta gcaaattttt aacacaaacg tttttcgatc cagtctgtga tttcctgaag
    29761 gcctttgtca aaattccgat acaccgggtt aaatgggatc tctaataatt taccgtcagc
    29821 agatgacgac gtgattaagc gtgagtcctc ttccgggctg aacggatcgt tcttccagta
    29881 gcctgataac attggcgttg ggcagcgacg tcccagcaac ccttgcactt ttaatgaata
    29941 gcgattcagc tccacgcgca acgcttcgtc agaagcatca tgcatcccca aacgactcgc
    30001 cagaacgtca agatacattt ccggcacctg ttgctggcac ttaaaatcac tcaacagggt
    30061 atgaactacc ggaccaagac aggcaaccgc tttcagacgc ggcgattcaa ggtatgccag
    30121 acgtacggca acgttagcgc cgaaacggaa accaaaggcc gcgacgcgag tgtgatccac
    30181 ccacggtacg ttaggcaacg cctttaaaac gtgctgatgc aacaggctgg agtcctgggt
    30241 gagcttccat tttgaagaaa agcccaccga cggcatatca atagtcagca tcgcaatgcc
    30301 gcgcagcgca aaataacgtt catacaggct gtaatagtct gtctgcatcg catccagacc
    30361 accacacatt aatactgtcg ggaacgggcc atcgcctttc ggcatatgca aaaagccggt
    30421 gatgggcgca ccgccgggta cggtaaactc catctgccgc atcgtgcccg gcagacgctg
    30481 agcggcctct tcataggcgc ggtttgacaa agcctgcgct tgctcggcca ggtcatctcc
    30541 tttcagatga ggataggcag caatgttgta caacgtagcc gcatgcagcc agtgtcgacc
    30601 gctgagttgc ggatcttctt ccgcacaggc tttttgttgc cacaccatcg cctgggtagc
    30661 ccactcataa atccagttgc cgccgcggta gccaatcacc gtatcgtata aatcatcgtc
    30721 ggtacgttcg gcatcgctca tcacaatacg cgcctggact tcgaggattt cacgggggtc
    30781 aatgccgcgc cagatccaca tcagacggtt aatcatgcga taccagtgag ggatggtttt
    30841 accatcaagg gccgactgca caggcagttg tgcgccgtga ctaaagcggc ggactagcgt
    30901 cgaggtttca ggatgtttaa agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt
    30961 catagatgca gcctccagaa tacttactgg aaactattgt aacccgcctg aagttaaaaa
    31021 gaacaacgcc cggcagtgcc aggcgttgaa aagattagcg accggagatt ggcgggacga
    31081 atacgacgcc catatcccac ggctgttcga tccaggtatt ttgcgggata tcaacaacat
    31141 aatcatcaac cagcggacga ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg
    31201 gatacatttc acgaatcgca accgcagtac caccggtatc taccaggtca tcaataacga
    31261 tgaagccttc gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt
    31321 gatcgtagct ggaaatacaa acggtatcga catgacgaat acccagttca cgcgccagta
    31381 acgcacccgg taccagaccg ccacggctta cggcaataat gcctttccat tgttcagaag
    31441 gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt
    31501 atttttcgct catgtgaagt gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg
    31561 ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt atttcacatg
    31621 agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg ataaagtggt attctcaaac
    31681 atatctcgca agcctgtctt gtgttgacaa cattttctgc taaccctgtg acctgcaata
    31741 ctgttttgcg ggtgatcgac aaggagactt aacgtgtctg aactgtctca attatctcca
    31801 cagccgctgt gggatatttt tgccaaaatc tgttctattc ctcacccgtc ctatcatgaa
    31861 gagcaactcg ctgaatacat tgttggttgg gcaaaagaga aaggtttcca tgtcgaacgc
    31921 gatcaggtag gtaatatcct gattcgtaaa cctgctaccg caggtatgga aaatcgtaaa
    31981 ccggtcgtct tgcaggccca cctcgatatg gtgccgcaaa aaaataacga caccgtgcat
    32041 gacttcacta aagatcctat ccagccttat attgatggcg aatgggttaa agcgcgcggc
    32101 accacgctgg gtgcggataa cggcattggt atggcctccg cactggcggt tctggctgac
    32161 gaaaacgtgg ttcacggccc gctggaagtg ctgctgacca tgactgaaga agccggtatg
    32221 gacggtgcgt tcggcttaca gagcaactgg ttgcaggctg atattctgat taacaccgac
    32281 tccgaagaag aaggtgaaat ctacatggga tgtgcggggg gtatcgactt cacctccaac
    32341 ctgcatttag atcgtgaagc ggttccagct ggttttgaaa ccttcaattt aaccttaaaa
    32401 ggtctgaaag gcggtcactc cggcggtgaa atccacgttg gcctgggtaa tgccaacaaa
    32461 ctgctggtgc gcttcctggc gggtcatgca gaagaactgg acctgcgtct tattgatttc
    32521 aacggcggca cactgcgtaa cgccatcccg cgtgaagcct ttgcgaccat tgctgtcgca
    32581 gctgataaag tcgacgccct gaaatctctg gtggatacct atcaggagat cctgaaaaac
    32641 gaactggcag aaaaagagaa gaatctggca ttgctgctgg actccgtagc gaacgacaaa
    32701 gctgcattaa cggcgacatc tcgcgatacc tttattcgtc tgctgaacgc caccccgaac
    32761 ggagttatcc gtaattccga tgtagccaaa ggcgtggttg aaacctccct gaacgtcggt
    32821 gtggtgacca tgaccgacaa taacgtagaa attcactgcc tgatccgttc tctgatcgac
    32881 agcggtaaag actacgtagt gagcatgctg gattcgctgg gtaaactggc aggtgcgaaa
    32941 accgaagcaa aaggcgcata tcctggctgg cagccggacg ctaactctcc ggtaatgcat
    33001 ctggtacgtg aaacctatca gcgtctgttc aacaagacgc cgaacatcca gattatccac
    33061 gcgggcctgg aatgtggtct gttcaaaaaa ccgtatccgg aaatggacat ggtgtctatc
    33121 gggccaacca tcaccggtcc acactccccg gatgagcaag ttcatatcga aagcgttggc
    33181 cattactgga cactgctgac tgaactgctg aaagaaattc cggcgaagta attatttgat
    33241 ttgctgccgg atggcgttta acgccttccg gcagtttcat ccttcattat ccttcgataa
    33301 aagccatccc tgtgaacgtt cgtcgcgggt tgccacgttc aatctggtga tggaacattc
    33361 gccgctgcga tttcagcgcc gcgctatttt cctgttgctg ttgttccagc ttccaggcaa
    33421 tcagtaatcg cgccagccgt ttgttagcat gctgactgcg ctctgactga accttcacgc
    33481 taataccgga tgccagatgc gtggcgcgta ccgccgagtc ggttttattg acatgttgac
    33541 cgcccggccc cgacgaacgc agcgtctcat aacggattgc atccgattgt tcctgctcat
    33601 cagcggtaaa acgcccaatg cccagaaacc agtttttgcg cccatgatga ggccgatacg
    33661 gactcggaca aatccactga atagtgccgc accagctttc gcttagagcc catgcgttat
    33721 cgccatccag agaaatcagc gccgaacgca gtgtgtcaga gtagcgaccc gtttctgttt
    33781 ccagcaccgt taccgcgacg tcctgtcggg tagcttcttt aatcagcctg tccagtgctt
    33841 ttttcactgc gagacaacat tcttccggcc cctgagcaga ggagagttgt agcaagatca
    33901 tccttttttc cctccactgt ttttgagcgt cagcaccggg cgcagtcgcg cgacaggaat
    33961 aattaaccca gccagcacca gacattgcac cacgctttca gccgatttat aggcttgtgg
    34021 cgcttcttca aagatgagtt gtttatcgcg acaaattacc cgactgccaa gttcagtccg
    34081 cgagagttgc gtcgccgtgt atttcgctgc cagacgccct ttacactcgg tgcgccccca
    34141 tttacgcccc gccccatgcg ccagcgagtg gagcgttttt tcgttcgcaa caggtttaac
    34201 cagccaggag taatcgccgc gtgaaccggg aataatcacc agaccgttgt catccggcgt
    34261 ggcgccttta cgatgcaacc agccctgttg atcgccgatt tggcacgcgc taacaaagtt
    34321 atgcgccaca tccagaaccg gacttccggt ggccttaact tgttgcatta tgcgcaaagc
    34381 gatcagctgg cgattaatac gcgcaaacgc cagcgcatcg tcatgttccg caatataccg
    34441 tagcgcgtcg tcactgcctt caggcaaacc atgatgcgaa aacgaggcaa tatgccgctg
    34501 taaaatagac tgccccaatc ccctcgagcc gctatgaacc agtagttgca gatgctgcgc
    34561 atccagacca gcccgtgcaa acagttccgc gtcgataatt tgatcaatct gttgcagttc
    34621 tgcgaagtgg ttaccgccac cgatggagcc aagcgagttg tgccacggat gctgtgcaaa
    34681 tgctgacggc aggttttcct ccagccagct ttcttcagca acgtcatcca gatctgataa
    34741 tcgcttttca aacttatcgg cgttgtattt gcgagcgaga atatctgttt gccatagcgc
    34801 catgccgcag ccgatatcat tgccgaccag tgccgggtaa aaacgaccaa cagagaagaa
    34861 cgctgcgcca atcgggtagc cgcgtccggg atgtaaatct ggcatcccca caacgcgctg
    34921 catgttgggt aaatttgccg tggtgtgtaa ttgttggatc gctaaactct cgatccacag
    34981 gtcatcagat gcgatggtaa ataccgcatc ggataaggga cgaatatatt tgcccatgta
    35041 ccattccatt tatgaataaa aatcaggaaa tggcagacga atccagggca agaaaaaaga
    35101 attaatcctg gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc
    35161 ttgcagtttg tggaataaga aaacgcgcac atactagacg ggcgttattt tcattgcaag
    35221 ctggatttaa tgttgcggtt tatatcgcat ataaaaatta gtaaaccacg ttccccggca
    35281 ttcaacgcgc tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt
    35341 tatgctggcg tccaccgtaa gttcggattc agacttaatc aatggtttta acaaagcgct
    35401 ggcaactccg cgtcgggtgt attccggttc aacaaataac atatcgatat aatgtccaat
    35461 gcgggtaata aaaccaaccg gttgtgcatt aataactgca acccgcactt gtgatttcgc
    35521 gagtttctcc ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gctgtggaga
    35581 atagtgctga ctcgccgtca tcgtaacagc tcgaaggaaa atagcgcata gttgctgaaa
    35641 atcaccaggc tgatagtttc ttatttgtat gttattcata atcccagcac cagttgtctt
    35701 tccatttgcg ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc gcgcccgccg
    35761 cggcgttcat cccaggtttt acgcgcggtg gcgattagat cagatttatt cagccgcggc
    35821 cagacgtgtt cctgggtggt ttgctgaaaa tcgtcgaact ttaatttcac cccctggcgg
    35881 gcaatcagca aatcaggttt tacctttgcc aaacgacgct ccagttcagg gtaaagccgc
    35941 tcgatgatcg cttcacattc agaccagtga tgaatatctt ccgccatcgt acgttctaca
    36001 ccgacggatt ttcgcaacct ttcgctgtta acgtcgcgtt cgtcaatgcc ctgactacgc
    36061 tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gcatcaccag atcacacttt
    36121 tgcacatcac cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg
    36181 acgccgggaa tttttgccag cggtaaggtt tgtaaaaatg tcggaacttc tgccggcgta
    36241 atcacaaact ggccgttggg tttattcatg tcggaggcga ttttggcgag aaactttact
    36301 ggtgccacgc ccgcagacgc cgtcagttgc agctcgttga agatcgtctg gcggatttcc
    36361 tgggcgatga gggtcgcaga accgtggcaa tggacgctat cggtgacatc gagataggct
    36421 tcatccagtg ataacggttc aatgcgcgag gtatagcgcg agaaaatttc gcggatatga
    36481 tttgaggctt ctttgtaggc gtcaaagcgc cctggaagca aggtgagatg cgggcataat
    36541 ttgagcgcca tccctgtcgg catagcgcta cgtacgccaa atttacgcgc gggataattg
    36601 gcggtgctga tcaccccccg tcgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc
    36661 agggcgggat tgtcgcgcat ctccaccgct gcgaaaaagc agtccatatc cacatgaatg
    36721 attttacgca ttgctcacct ctcaatactg gtaaagtata cagtgatttc agggtttgag
    36781 aaatgcgtaa agattcagca tttaacgcgt tcgctgcgaa agcacctgct gcttatccag
    36841 cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt
    36901 atcggacgca cttttggtca gcaccataat ctcaatgcgc cggttgcccg cgctttgcgg
    36961 atttttggca tccagcagca tctggtccgc cattgcactt acctgcatca ctttatcttc
    37021 cggcattccg gcctcttcca gcacccgacg agctgaaagc gcgcgatcgc ccgaaaggtt
    37081 ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg taataataat
    37141 tttgttatcg agcgagtcga acactggcgc aagctccacc agcaaagttt taaagaacgg
    37201 cataatcttc gcgctgccgc gttcgaacat attgcggttc tggtcgtctt taatcagcac
    37261 gcgtaatccc tggggaacaa tctccatctc cagattcgct tccatatgcg catcacgggc
    37321 gatggtattg atactggttg ccagctcgcc aagctcggtg gcggatttct tcttcagcag
    37381 cgccgttttc tcgttgacgt cgcgcgcttt tttctccgtc tcttccggcg ccgccacggt
    37441 ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacaacc caccaccgtt
    37501 aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct
    37561 gttgacaatc cacagcgtca taaatagcgc catcattgcc aaagtaaagt cggcgaacgc
    37621 caccttccac gccccgccgt gatgcccggc atgattcttt ttgatctgcc gccggacgat
    37681 ggtggttttt gccccacgtt cgcggtggtt gcccgtcttt ctcatgctta ttcttgctcc
    37741 agcatcgcat tcacccacgc gtcgaggttg gcgaaggtcg gtttggacgc cagatgcagc
    37801 agcttacgcc cggcgtccac cgccagcagc gtcggtttgc cgcctgcctg cgccaccagc
    37861 acagtgcgca cgcattccag caaagaatgt tccgcccgcg cctgctgttc catggcgttg
    37921 gctagcgggt ccatcaggca gtagcagata aacacgccta aaaacgtccc caccagagcc
    37981 gccgccactt tcaggccaat cagagcgata gatccatcaa tcgactgcat ggtgatgatg
    38041 atgcccagca ccgccgcgca aataccaaat cccggcatcg cttcggcggt acgttgcagc
    38101 gagcgcgacg gcgttaacag ggactcttcc gcggtgtcca gttcctgatc gagaatgcct
    38161 tccagctcat gggcgtcgat tttacccatc gccatcaggc gaaagttatc agcaataaag
    38221 gtcaccagcc gcttctgggt taacaccagc gggtatttct ggaaaatggt gctctcttcc
    38281 ggctgctcaa tatgctgatc caacatccgc agaccgccgt tttgcaccat ttcgagcaac
    38341 tcatacaggc acattaatag ctgacgctga aactccggcc cgagctgttt tttactgatc
    38401 acgcctttaa tttgatgggc aatctctttc agcacatgtt tcgggttacc aataatcatc
    38461 gccccgaaac cggcacccaa aataatgatc atttccgccg gttgccagat agccactaat
    38521 tgcccgcccg cttcaaaata accgccaaat acgcagccga gcaccaccag taaacctaat
    38581 attttttgca tgatttcttc actttaattc ggatagacaa aatcacgaat tttctgcgcg
    38641 atctgtttat tcagttgaca aattcgggcc tcggttagcc ctaataccag cgcaatctct
    38701 ttgagattca tctcatgctg gtaatacatg gagaggatca gttgctcttt ttcgcttaat
    38761 tgtgaaagcg cgtgttgcaa catttgctga gtgacaaact gatcttccag ttcccggcca
    38821 gcaagcggaa tgcctggccc ttcgccgttc agcagttcgt ccaggctggc gagcgtttcc
    38881 gccccttcca gttgctgata ttcgagataa tcttcatgcg agatcccttc ctcagccagc
    38941 tcctgccact ccggttcgtg ccccagtttt ttacgcgttt cgcgaatcag gtctttcatc
    39001 tgatgatatt tctgtcgcag ctggcgcggt cgccagtcga gagcgcgcag ctcatccagg
    39061 atcgcaccgc gaatacggtg cacggcatag cccgcaaaac cttcgtccgg cagcccatag
    39121 cgacgaatgg cgttcaacag ccccattaac gccgtttgtt ccatatcctg acggtcaata
    39181 atgcaattac actgcggggc aagttgccgc accactttac gcaccaacgg taaataggcc
    39241 tgcaagtagt ggctctcctg ctgcggagtt aataccagcg tggctgcaaa ttcttctgac
    39301 tcgaatacat cctgtagcat gtttatatcc gcctggtgat tactggaaga ccactttttt
    39361 caccagcaaa tccttatacg gtgcggtcat tttgcgacgc tgaagatctt tctttaacgc
    39421 gtcagaaatc agtttgcgga tggcagatat tttcatgccg cgcaaatctt caaatttcat
    39481 ttccgtcagg cattcaaccg caatactttg atacagcggt tcctgatgct ttattttttc
    39541 cgcctctttg tcactggcga ccaccatcac taattccgcc gacaaataat gatccgcgcc
    39601 gttgttgtca tgcaaagtga caatggtttc cggcagcgtc acaaatacgc tcttgctctc
    39661 atccagagct tccaccgatt ccgtttgcgc ggcggtagtc ggttttcctt ttccggcata
    39721 atgatgccag acactccatc ctgctccagc gccgaccacc aacgccagaa cggcgctgac
    39781 cacgcctgcc accacgattt tcttcatatt gcctttacac tttgatcaaa acggattcgt
    39841 tctgttgaaa ttgaggcgca tcatcaagct gtaaggcggc actcacctct tcctgctgat
    39901 ggcccgactg ttgttgctgc tgcgactgcc cttcggagga aacctgtacg ttcacctcca
    39961 taaaattttg cgccgtcaga tgctggcgga gatcgtcgct aaactgctgc aatgcgcggc
    40021 agacttcgct ttgattcgca ccgatatgga ccgtcagttt gccgttatcc aactgtacgg
    40081 cgatctcaag cttgccaagc gacggtggat cgagacgaat ggtggagatc tgctgttgtt
    40141 tgttgagctg aaagtggatc tgatctttca acagcgtcgt cagtttttcg cccagttcgt
    40201 ccatcgaaag cgtggctggt ttggcgacaa tctcctgatg atcgctgacc aactctggcg
    40261 tcttaacctg caacaccgca cgcgcaataa ccggttcgct gtccggcact ttttccgttt
    40321 tacgctcgac ctgcgggcgc gcggtcacac gggttagttc aggtttcggg cgagctggcg
    40381 gtgttgagac gcgcggctgc gtcggcgcaa tggcatgtaa atcttcactg gcgtaagtcg
    40441 ccagtctggc ctgctgttcg ggtttcgcct gcggcaactg accgatcagt tcctgcaact
    40501 gcggcggcaa cgacgtgagt tcctgctgct gcgtcgggcg ttgcggcgcg ttttgcacca
    40561 ccgctttcgt caattgctgg ataaccggcg atgttgcgac gtttcgcggc tgcggcgtgt
    40621 cctgatgcgg cgcggcaggt tgcggaagta acagcgccat tagcgcctgc atcgccaccg
    40681 gatcgttctc ctgcgggcgc gtcgcctgtt gctgactggt tttagcgcta tgaacgcgct
    40741 ccgccaccgc cgcgaccacc attttcggca gggtaaacac gggcgcgttt tcaccgctca
    40801 ccggcggtaa aatatccgcc ttcaacgaag cggtttccgc aagcgttccg agcgtggcaa
    40861 gcaatgccgg attcatgact tctccgaaag taaattaaca attgagtagg ccagtacgcc
    40921 ttcctgatgc cgctgatgct gttccatacg cgctttcaac acttctgcgc gctgctcgcg
    40981 ccgtttaatc agggcattaa acgcgccgta cagttgtgcc ttcaccgcat ccaactgcgc
    41041 cgcgtcggtt tccgccgccg cgtaatcgtg aaactggcgc atcaactgct gatacagtgc
    41101 cggaaggcgt cgccagcgtt gcttgtcgag ggcgtcattc atcgcttcga ccagtgcaaa
    41161 aagttgcctg cgttgttttt ccatcgtctt aacccagctt gcccgaaagc ccttcccagc
    41221 cttcgcgcag gttgcttaag atcagcatca cttcatcgat tttctctgcc gacagttcgc
    41281 cgctggcttc atacaagcga tacacacagt ggtcatagag gcgcgaaagg ttgaccacca
    41341 gctcgccgcc ggtttcaaat tccagcgaac tggtgagcgc attgagaatg tcgatgcatt
    41401 tattgatgct ctgggctttc ttctcgaaac gccgcccttc aatgtggctt ctggcgcgtt
    41461 ccagctcgtc catcaggcca gaaaacagca ccagcaccag ttcaagaggc gatgccgccg
    41521 cggtacgcgc cgcgagatcg atctctttgt attgcgaata accgtcctgt acttggtaca
    41581 tctttaagga tattccttct gaattacgca aatgccgcca tactggcttt catggtgtac
    41641 acctcaacca acgtattggt gtactcctcg agataacggt cgtagttagc gttataggta
    41701 tcggtaagct gatcgccttc gtcctgaatt ttgctcatct gatcatcaat gttctgctgg
    41761 cgcagggtga tgatgccgtt gctggagtcc gtgtaggtgt taatcaggtc gtccatctgc
    41821 gccaccatgc tgttgtcgcc aacgaaaata gaggtcaggc cgtcgggatc gctcttcatc
    41881 gcgtcgttga actggtcgga gtcaatctgt aagtggccgt gagaatcgag cgtgatgcca
    41941 tagtcgacaa tcgacacgcc gttgtagctg gcatgggcga tgtcatcgag ctggtttgcc
    42001 agtgagctga gtccagcatc ccccgcaaag accccggcgc tactactgtc atcaccgtgg
    42061 gtggttagcg aatcaaccgt atcaatcaag gtgttatagg catcgacaaa ggtctgtact
    42121 ttctcctggc tggcgctgga atcttcactg atgttaaagg tggtgagatc gctgtcagaa
    42181 tcgctgactt cggtaaaagt catggtgaca ccggggatca catcatcaaa cgtgttgctg
    42241 ctgttggtga tagccggtcc gctggcgcta ccgagatgaa taatcgcgtc ctgagcggag
    42301 gaaacatccg tggcaacggg cgcactggtg ctatcgttgc tggcatcatg ccccgttacg
    42361 ctcaccgaaa acgcgctctg cgccccggtg ctatcggagg tgagcataat cgtggtggtg
    42421 ccgtcggttt ttaccagtgc cgccgacacg cccggattgt catcggagtc gttaatcgca
    42481 ttcaccagct cgctggcgtc gataaaaccg tcaccgtcac cgttctgatc tgccgccgac
    42541 agatcgatat ccatagtgct gtcgcccatc gtcagttcaa aagtaccggt agcggaaaag
    42601 gcgtcgtcgc ccatactgaa cgtggtttgc tgcccctgcg ccagttgctc aacgaaaaac
    42661 gagtagcttc ccgcctgcgc ctgggaattg gcggaaacgg tcgccgagtc attattgctg
    42721 gtggcggcaa aagtcaccgg gccgtcggta tcgctgttca gcgcgtcaac ggcgctctgg
    42781 aaatcgctca acgccgagct gagcgagtcc aggccgctgc tttcagcatc cagctcactc
    42841 tgcttctcct gcaaattcgt cgcctgagta gcgacatccg catacgcgat ttcttgcgca
    42901 atggttcttg ggttgatcat tgatgactcc ggataacaat gtcaggttat tggcaaaagg
    42961 cgtgccaata ttttttatgt gaataatcaa tagattgaaa aacaggcgga aatgacgttt
    43021 ccggtctggc ggaaaaaagc ctgccgcaga aagcacaaca ccgcccacag gcggtgttgt
    43081 tcgtggccgg ataaggcgtt tacgccgcat ccggctatcg gtgcacagtg cctgatgcaa
    43141 cgctgacgcg tcttatcagg cctacgagtc cgcgcctgtt tttatgcgtg tgcccatttg
    43201 taggccggat aaggcgttca cgccgcatcc ggcaatcggt gcacaacgcc tgatgcgacg
    43261 ctgacgcgtc ttatcaggcc tacgagtccg cacatatttt tatgcttgtg cccgtttgtc
    43321 ggcccgaact tactgcagca ggctggaaac catgctggac atgctgttgg actgtttcag
    43381 catggtgata ccggtctgca tcagcacctg ttgtttggtc atattggacg cttccgtcgc
    43441 gtaatcggta tccatgatgt tgccgatagc cacttcggtg ttgtcctgca tgctggtcag
    43501 gttgttggcg gtgtcattca gacggttgat agatgcaccc aacttggact gaatctgaga
    43561 aacgtcatcc atcgcggtag aaatgctggt gatcatctgg ttagccgagc cagacgcagt
    43621 cagttcagta ccgccagaaa cgcctgcggt gccagtggtg tcagcctgat ccgcactaaa
    43681 ggagttgctg atagcagaaa ggtcagtcac cagcgtgttc agttggcttg aaatgttcac
    43741 ggtcatggtg tcggaagatt ccgcgcccac ctggaaagtt accgcgctct ggaacagacc
    43801 gtcagtaccg gtgttgctgg tgccggaaac accgaacagg ttggtgccgc cgtaagtagt
    43861 gttttgcagc atgtcagaca tctgctggcc cagttcgtcg tattcgtcct gcatcgcctg
    43921 caggtcgtcg tcgctgtagg tgccgttcgc tgcctgcgtt gacaggtctt tcatacgccc
    43981 cagcacgtca gacatttcgt cgaacatgct gtcagcggtt tgcagcatcg cggtagcatc
    44041 attgatgttg ctcagcgcca cacccatacc gctggactgt gccgtcagac ggttagcaat
    44101 ttgtttgcct gcggcgtcat cggcagaaga gttaatccgg ttacctgtcg ccaggcgttc
    44161 catagaagta gacagagaag agctgctttt gctgattgca ttaacggcgg ccattgaggc
    44221 gttattggtg ttaatagata acatgatact gctccttggg attaatcgtg ttcgtttcga
    44281 taccctgtaa aaacgacacc tcgggagcaa acttaaaatc gccgttaaaa ttttttcgcc
    44341 acgtattatc cgatgccact tttagctaaa aaaagcagag tttaaattca gataaaaaga
    44401 ttattagcaa tatctgccac atggccaggt atcaaaatat gtctttatca aatgcttgat
    44461 atagttaaga ttgtcatcaa ttaacgcgtt ttcttctcaa tgtactgatg ccaaaattta
    44521 cattgcgttg caaatagtac acgtttggct attgtacctg tcctgtcatt tcgttacatt
    44581 tccacgccat atagtgatta agaataatcc tattaccata aaatattaat tatcacgcag
    44641 aaaaatcatc ttctcaggga cgtataagac tgtgctttat gcaaagcact atcaggaaac
    44701 atttatccct tgctaaggta aactcaacta tgagtattat tatcaacaac tggcggatgg
    44761 acccgtcgct taatgcctta atacattgcg aaacgggtga aacacgtcgt ctcggcgaat
    44821 accattttat tttgctggaa acattagcaa aaaatgccga tgtggtttta tcgcgatctt
    44881 atttatgtgc cgaagtatgg aagaaccgca tagtcggcgg taacagcctg ccgacggcga
    44941 tccatgcgtt gcgcgttgct atcgatgatg atggcaaaca acagaacatt attaaaacaa
    45001 ttccgaagaa agggtacctg tgcaataaag agtatgtgtc ttttccggag tcatcgcctg
    45061 cggaagcgct gattattacc gaccagatac agaaaacaac gccagaagag atatcttcga
    45121 caacaccgcc tgtacctgtt agaaaaaaac ataaaggact gatggggctg gcgttggccg
    45181 cggctgttat attggttggc tcaaccgtgg ggtattcaca cttgaaaagt acacctgacg
    45241 ccccgcaact ggtaaaagag tcaattaaca gtccaagaat aaaaatattt catcttagtt
    45301 ctggaaagga aaataattcc gtaccgttac tgtcacaaat cctcgcacca ggaaaagaca
    45361 aactcgataa tttattgtca gcgcataata tgacaatgac aacctattat aaatatgttc
    45421 gtaaccgact ggaaagcgat attgttctgc gtaaccagtg caacggcagc tggcaattaa
    45481 cctttaatgt ggaaggttgg cagaacagcg atattaatag cgcgatgtat cagaatttag
    45541 agaagttgtt aaatactgtg cagaaatgct gacaagtgcc aatatatgcc ggatgcgacg
    45601 ctaaacgcgt cttatccggc caaccgataa ttatcccaat aaattcctca ccgtctggct
    45661 ggaaattcgc gcctgaccgt tcaccgcctg caataaaatc tgatagttat gattggcgct
    45721 atccagcacg ccgccgaggt tctccgatgc ctgcaccgcg ttttccgttt gcgggaagcg
    45781 tgacatgccg tcgatcaact ggcgggcttt ttcctgctgc accgccatct gcgcgcgctg
    45841 ctgattaatg ctttctaacg tctgggaaat ccccgcccca ccctgttgca aactttgcgc
    45901 taaacgcacg gcctgcgacg gttcggcaaa ggtttttagt ggcataaacg ccgacttatc
    45961 accatcggtg cgcacgctta aggtgcgctc gatgtcaggc cactgttttt cggtggtgga
    46021 gaaactgatg ccgtccgctt gctgatgtac ctgcacgccc acgcggcgca gggcgttggt
    46081 caggcgcgtc tggtactgcc cggcgctgtc atcctcagac agcatcaccg ccgaaagctg
    46141 ggtctgccgc ccgtcagaga cactgaacat gcgcgttccc ggcgtgggat tgtgtaccag
    46201 attcgccaga tccggcgagt ggaaggtcac ccgcgcttcg ccctgcaaca ctggttgtaa
    46261 ctcgcggtcc accgcgccgc cggaaagcgc ggcgcgtttg tccagcatct gcatcagcgc
    46321 cgtactctgc gcctgcccgc ccttacgctg gctgtgacga tagtcgagca actgctgttc
    46381 cagttgcccg agatagtgat ccgcctgctg caacgtggtg agctggtcgt taagctgtac
    46441 gctgtaacgc tgcgggcgag tggtaatcag cggcgaggcc ggatattcac tcgccgtcgc
    46501 cgggagtttt tgccgcaccg cctgagtgga cgcaacggtt gacgacgata ccgcagcgct
    46561 atgtgcgccg ccggtggcga gtgatgaaga tgtaagtccg acctgcatac tgttcctgaa
    46621 agagttagag aacgctgaac aaattcaact cactcacttt cgagtaagtc ttgagggtag
    46681 cttccattgc cacttccagt ccggtaaagg tgatcgacgc cggaccgtaa tcgagatcct
    46741 gcaatgaacc aatcaattca tcattagatg tcgagatatc ggtctgtgca tcgctcaaca
    46801 tcgacatggt gttttgtgtc tcgccgagcg acgcaatcga ggcgttgagg tcgtccgagg
    46861 cggtgtccac cacatcgacg gcgttctgaa tatcgctctg cacctgcgga tctgccgggt
    46921 cgacgtccgg gttttgcagt tcctgcgaca aggaattaag cgtgttaagc acgtctaaat
    46981 tgctaccgaa aaaatcgctc gccgccacgt tggtgtccac ttccacaccg ttcgacacgg
    47041 tagtctggcg atggtcgctg ttgccctgat agctatagct gtcggtgacg ccatcgccat
    47101 catcgtctac cgcgacaatc ggcggctgat cgttgatggt gccgccaaac acgtagtgcc
    47161 cgtcttcgtc ctgataattg agcgccgcca ccattgaatc ggtgagcgac tcaatatcct
    47221 ggccgaggct tgccagcgaa tcggcggtat tggtgccgtt cgccgcttca agcaggtcgt
    47281 cgcgtaccac cagcaggctg ttgttgacgc catcgagaat cgattcctgc tggcttaagc
    47341 ccgccgacgc ggagtcgata ttgctctgat actgttcaat cgccgactgc tggcggttta
    47401 actgggtgat gcgcgtggcg gcgattgggt catccgacgg ctgcaaaatt tccttgccgg
    47461 ttgccatctg ctcgaccaca tgcgccagag agccggagag attattaaaa ctttgcgtca
    47521 tcgagacgta ggtttgttgg gtggttactc gcattttcgc gctcccgctt agctgcacat
    47581 ttccagcacc gaatcgaaaa tctcggcccc ggcggaaatg actttcagat tagcttcata
    47641 aatttgttga taggtgatca ggttcaccgc ttcttcgtcc atgctgacgc cgctgacgct
    47701 actctgctgg ttttgcgctg cggaatagac attggacgcg gcatcgactt ccgtctgatt
    47761 ttgctgactg taaatgccga tattgctgat gattgacgag cacgcctgcc cgaccgtcac
    47821 actgccgagg ttggctatct ccagcggttc ggtggagata ttgatcagcg cctgcaggtt
    47881 gtcgctgttg ccgctttcgt ccggtgaact ggaaaacgcc agctcatcgg cagtgatatc
    47941 cgggttaacg gtcagtgggc catcggcatt gctggcgtca taaataaaca gtggctcgcc
    48001 ggggttaccg ttgagatcgt agccctgcgc cagctggttg ttgaccgcat cggcgaactg
    48061 cgacgccatg ctgttgatgg tgtcggtcag cggcgtcagc acgtcgtttt gatagtcgaa
    48121 cagagcgcct aacgaaccgc cagtgtcggt ggtcatggtc gaggtggtgc cagcgaaagt
    48181 cagcgacatg gtcggtgtgc cgtcggcgtt ggtttccagc gcgatggtcg agctttgctg
    48241 cccgctcacc agcggctgac cgtttttcag ggtgacgttg tagttgccct ggtcgtcaat
    48301 attgacctgc acgtccatca ttccgctcag ttcttccacc atctgatcgc gggcgtcgta
    48361 cagcgcggag gcgttatcgc cgttggcttc ggcctgagcg atttgctggt tatagctggc
    48421 gatgccgctg gtcagcgtat tgatttgcga taccatcgcc tgctgctgac tgatgatttc
    48481 cgtgctttgc gagtcgatgt aatccagcgt gttgtcgatg cgcaacgaca gcgccccggc
    48541 ttcgctgatc acctgctcgc gcagggcgga atcatcgggg ctggtggtcg cttcgttaag
    48601 ggcagcgaaa aagttatcga agccgccgct cagactgctg ttatcgtcgc tcagtaccgc
    48661 ttccagttgt gtgagatacc cttgttgggt gctgtaatag ccgtaatcgc tggcggcata
    48721 ccatacctga ttaacctgat actggttgga gacgcggcga atactgtcga cctgcacgcc
    48781 gttaccagcg ctgttcggcg aaccaccgct ggcaccgatg gtgctgatct ccgccacctg
    48841 gcgggtgtag cccgtggtca tggcgttagc ggtgttttgc gccgtgacgt tcagctccac
    48901 ctgcgccgtt gatgcgccgc tgtagccgat gttaatcatg tccatgttgg gtccttaccg
    48961 gtaaatcgtg ccggttaaaa gcggcgtgaa gttggggatt cttaagttcc gtaaaccggg
    49021 tgccaaatgc cggatgcggc gtaaacgcct tatccggcct acatactaca tacgggcgac
    49081 tcgtaggcct gataagacgc gacagcgtcg catcaggcac cgaacgccga agcctcattt
    49141 atgcctgggc gacaactgct tcacaagcat cgccgcaatc ccaatcccgt gctgctgcgc
    49201 cagggtgtta cacaactcat cgtcataaaa cccctgcatc atgcgcaccg aatcgctgtt
    49261 aaacgggtta tctttcgaat caaacaactc gttggtttta cgcatctctt tcagcatgtt
    49321 gcgtaaaaaa atcgcctcaa actgctccgc ggcctgctta atatcattgg cctgaacctt
    49381 cggccccatt aacgcatcgc tgccgtcaat cccgccgctg gcgttcactt tcatcagatc
    49441 acctccaggt cggcatccag cgcgcccgcc tcatgcagcg cctgtaaaat cgacataatg
    49501 tcgtccggcg ttgcgcctaa actgttcagc gcattgacca gcgttttcag gctgctcgac
    49561 tccggcaaac tcaccacgcc cgggcgcgca tgattcaccg cgatattgct ctgcggcgtt
    49621 accgccgtgc gcccgcccgc aaaagcattt ggctggctga cgttgctggt ttcgttaatc
    49681 gagacggtca aactgccgtg cgacaccgcc gccgcatgca gcgcaacgcc atcgcccatc
    49741 accaccgtgc cggtacgcga gttaaacacc acgcgggcgc ggattttctc tgcctgcacc
    49801 tgtacatcat ccagttgcga cataaatgcc acccgcgcgc cggggctggt gggcgcacgc
    49861 acgctgacgt tggtagagct ctgcgcagta gcaatgccgc caaacgaggc gttaatcgcc
    49921 gccgcaatat tgttggcatc tttaaacgac ggacgtttca ggttgagggt gatagtgtcg
    49981 cccatctgaa aatcgctggg gatctcgcgc tctaccgttg cgccgttagg aattaacccg
    50041 gcggttggcg tattaaccgt tacgctcgaa ccgctggccc cggtggcagt catcccgccc
    50101 accaccacgc tgccctgcgc cagcgcataa acttcgccat ccgcgccgtg caactgggtc
    50161 agcaacaaag tgccgccacg aatacttttg gcatcgccaa tcgacgaaac agtgacatcg
    50221 atggtctgcc cgcgcgagta catcggcggc agcgtggcgc tcaccgctac cgcggcgacg
    50281 tttttcacct tcggatcgat tttatttggc agttgcacgc caaactggcg cagcatattg
    50341 gtgatggtct ggttggtgaa tttgacctgg tttttatcac cagtaccgtc gaggcctacc
    50401 accaggctgt agccgaccag ttggttttca cgcacgccct gtacgttgac caacgactcc
    50461 agcgattgcg ccagcgccac gccgggcagc gtcagactta cagcgatcgc tgcggccttt
    50521 atccagtttt gcattgcact ctccggcatt aaatcgggaa cagaggatgg ttaaacagcc
    50581 gcgtcaacca gcctgcggcg ttggcgtcgc ttaacgcgcc gcgtccggca taggagatgc
    50641 gggcatcggc gatccgctgc gaggaaaccg agttgtcgtt ttggatgtcg tcggcgcgca
    50701 ccaggccgct taagcggatg tactcatcgc cctggttgag agtcagccat ttttcgccgc
    50761 gaatttccag gatgccgtta ggctgcaccg catggacgga aacggtgatc tccccgcgca
    50821 ggctgttctg ctgctgggaa gtggcgctgc cgtcaaagtc gcggttggcg tcgatagagc
    50881 cggaaagttt atctttggtg tgtccaaaaa tcgtgggcgc gccgatatcg acggtgttgc
    50941 ttttgccgaa gttggttttc gcctgtttgc tcgactgggt ggactcttcg aggatcaccg
    51001 tcaggatatc gcccacgcgg taagcccgcc ggtcggcggt gagcgaccag ttgtagccgg
    51061 tttcaaacac gcccccggca cgcccgtctg ccggaggcgg cgcttcggtg gttggtggcg
    51121 caaagtatgc gtcgtctttt ttgaccagaa tggcctgcga ttcacacccg ctaaggagtg
    51181 gaagcagcgc caccagccag agataatttt tcacgttctg cctgtttaaa tacagtgagt
    51241 tatgtgctct tgtaggtcgg ataagacgcg tcagcgtcgc atccgacatt tcggccttac
    51301 agcgtctggc tgatgtactg caacatatcg tccgcagccg agaccatttt ggcgttcatc
    51361 tcgtacgccc gttgcaccgt aatcatcgcc accatctcgt tgacgatatc cacgttcgat
    51421 ccttccagcg cgttgtcctg caaggtgcca agcccatctt cgcccggtac gccttcggtc
    51481 ggctgaccgc tggcggcggt ttccagatag aggttgtcac cctcagcgga aagcccggcc
    51541 gggttggcga agttcaccag cgtcagttgc cctaactcag tcacgtcgct gtcgccgggt
    51601 aagatcgccg tcaccgtgcc atcctcacca aaagcaacat tagtcgcccc cgccggaacg
    51661 tcgatttccg gctgcaacgg caagccctgc gagttggtca gtacgccgtc agcgttcacc
    51721 tgcaagttgc cgtcgcgggt ataagcgata tcgccgttgg ccttttgcac ctgcaaaaac
    51781 ccctgcccca taatggcgac attcatcgcg ttgtcggtgg tttcgacgtt gccttcggtg
    51841 aaggttttct gagtgccgac aatgcgtacg ccgctaccga attgcagccc ggtcggcata
    51901 atgttgttct gatcgagcat cgcgcccggc gcttcctggg tctggtaaaa cagatcctga
    51961 aacatcaccc ggtcacgctt gaagccggtg gtgttaacgt tggcgatgtt gttggcaatc
    52021 gcgctcattt cagcatcctg cgccgagagg ccggttttgc tgatccatag tgctgcgttc
    52081 atggtggtaa attccgtgtt attttttggc cgcggttgcc ggatgcggcg tgaacgcctt
    52141 atccggccta cgatgtgcga ttacgagccg cgcaataagc ggttgcccgc gtcgctgata
    52201 tcttcggcgg ttttcatcat cttgatctgc gcttcgaact ggcggttcat ggcgatggac
    52261 gacatcatct cgctcaccgc cgagacgttg ctgccctcga gaaaaccgcc gctgactttg
    52321 atgttttcat cgcgctgggc cggaacgccg tcggcagtca ccagcatccc ttcaggattt
    52381 ttcgccaggt tcgcgaccgg gatatccacc agcttcaggc ggtcgatatc catcgtcgcg
    52441 gtcacgtcac cgtcatcggg cgtcacggag agcgtgccgt cgctgccaaa cgaagcaatg
    52501 gcgttgggcg gcagaatgat cggcccgtta tctcccagca ccagattgcc gtcgatagtc
    52561 agttgcccct ggtcatcctg ctgaatgttg ccgttgcggg tatacacttc gttgccgttt
    52621 ttatcctgca cagcaatgta acccgaaccc tgaatcgcca cgtccagtgg gcgttcggtt
    52681 ttctccgcaa caccagtgct gtcgttcacg ccgctggccc cttcctgcgc catatagcgc
    52741 gtgtcgaagc cgccgccttt caccttatcg ttagtcgcca ttgccatatc ggcgcgaaaa
    52801 ccgttggtgt tgacgtttgc caggttgttg gcgctgatct gctgctcgta cagggtctga
    52861 gaagccccgc tcagggcggt ataaatcaag cggtccatta cattgcctgg aacagcgcgt
    52921 catcgagctg cgtgctggtg gcgataactt tggtgttggc ctgatagtta cgctgggcgg
    52981 tcatcaggtt gactaactcg ctggtgatat cgacgttaga gctttcgagc acgcccgacg
    53041 acagcgtgcc gcaggtgccg gaaccgggaa cgccaatcag cggggtgccg gattcgccgg
    53101 tttgtaccca cgcggtgccg ctcactgcct ccaggccgtt ttcattcggg aacgtcgcca
    53161 gcaccacctg cccctgcaac atgcgctcgc cgttgctgta ggtagcgtaa actttgccat
    53221 cgtcatcaac ctgcacaccg ttctgagtgg cagaggcgta gccgtcggcg gcgttggtgg
    53281 tgaccgaaaa ttcagagccg tattgcgtac aggtcgagta atcgacggtt aaatcgatgg
    53341 gtgcggcggc gtcggtctga aactcaatgg tctgcggcgt ggttggcgag gtcagtttcc
    53401 cggtgttcgg gtcgaaggtt aacgtcgtcg ccggaacgcc ggtctgctgc tgaccgtcga
    53461 aggtgtactg cacttcccag gtgttgtcgc tggttttggt gaagtactgg cataccgaat
    53521 gttcgttgcc taatgagtca tacaccgtgg tggtgtagct gtcggtgtac gagctgctgt
    53581 tggtcgcgtc gaacggtacg gtagtgcggt cgatagccgc atcgctggca tcgaagttgg
    53641 cggtaaaggt caggctgctg ctggcctgcg ccggaatatt accggttttg atctggatat
    53701 cggtgactgt accggtttgc agggtgcccg tgtcatcgac cggatagccc tgcaaataag
    53761 cgcccgaggc gttgacgata tagccgtttt tgtcggtttc aaacgatccg gcgcgggtat
    53821 aagaaatgtt gcccgcgctg tcgcaggtaa caaaaaagcc gtcgtcgtta atcgccagat
    53881 ccagcgcgtt accggtggat accagcgaac cgccgcgcga aatactttgc gcggtgcctg
    53941 ccacgctgac gcccatcgcc tgacttccgg catacatggc tgaaaactgg gtggtcatcg
    54001 acttatagcc taccgttccg gcgttggcga tgttgttact gatcccgtcc agctgttcgt
    54061 taacggcatt cagccccgtt gcagcaattt cataactcat tgtgtcttcc tgttaattca
    54121 gattttggca gcgtgcccgt tgttttgccg gatgcggcgt gaacgcctta tccggcctac
    54181 gaacgggtgc aaacataaaa atatgcgcga actcgtaggc ctgataagac gcgttaagcg
    54241 tcgcatcagg catcttgcac cgaacgccgg atgcggcgtg aacgccttat ccggcctaca
    54301 aaaccattcg gattcaatag tttcatgtag gcctgataag cgaagcgcat caggcaattt
    54361 ggcaccggac atcacgccac atcactgtcg gcatcgggta cgccaaactg gctaatcatg
    54421 gtgaacggca cttcgcctac accggcaacg ttaagcaccg gggagctgcc gtcgagcggg
    54481 atgcgcacat cctccacttc gccggaaact tcaatcggca cctcttcttc cccggtgttg
    54541 gtgaccaccg agacgtcgta atcgcccggc gggataccgt aatccgccgg attaatcgag
    54601 aaatccacct gacctggccc gacggacgaa ctgccttccg ggatcagcga caccgtatag
    54661 tcatcgcctg cggcatcggt aaagtggaga tcggcggtgg tacaggcgtc gtcaagcgtg
    54721 gcgcgaccat taatggtctg gtcgctcacc tgcaaggcgg tggtctgcac cataatgtcg
    54781 tcacccacca ggttgccgag cgccatcacc tgaatgttgc tcatcagcac cgcgttggtg
    54841 ttggcctgca cgctcatctc ttccatcatc gccacctgcg ccatcgaaga aagctggtcg
    54901 atatattcgg tggcgtcagt cgggtcggtc gggtcctggt tttggatctc agcgacaaac
    54961 agcgtcagga aggtatcgac gggcgagcta tcgctgctac tttgcgtgtc gctggccgcc
    55021 acgttgttgc tttccgccgt ggcggtcggc tgcggtgatt gcgcattcag cgccagggta
    55081 ttcatcaggc ttctcccagt ttcagcaaac tttgctgcat acttttcacg ttgttgagca
    55141 catcgacatt ggtttcgaaa tcgcgcgaag ccgacatcat gtccgccatc tgttccacca
    55201 cgttgacgtc gggataccag acatccccgt tagcatcggc gagcggcgaa tgtggttcat
    55261 agcgtttcac agctccgccg gtttgcagca cgtcctgcac ctgcacactg gcaccgtcaa
    55321 tggcatgacg atgtgtaccc gccagcaaac tgtggtgata caccgccgca aacaccgggc
    55381 tgcgcgcttt atatgcctgc gcctcgctgc tggcgggcgc attggcattc gccaggttac
    55441 tggcaacggt attcagacgc agcgtttgcg ccgtcatcgc cgaaccggaa atctgataga
    55501 tatcagtaaa tgacatgctt atttcccttc aatggcctct ctgatgccgc tgatttgcag
    55561 attcagaaag gtcagactgc tttgataatc catactattt tgtgaaaacc gcgcttgttc
    55621 gctgttcagt tccacggtgt tgccatcttc cgacggctgc atcggcacac ggtactggac
    55681 atccaccgcc gcgttatttg cccgttgcat ctccctggca aaatcaatat ctttagcctt
    55741 gaaatttggc gtatcgacgt tcgccagatt cgcagtcagt agctcggtcc tctcaagacg
    55801 taacttcact gcctgcggat gtactcccaa tgcctgctga aaactgatcc ccatttcctg
    55861 cgtcactcct gcttcaatcc gcatacgcta agcaatcgcc gtgccatttt ttaagtgact
    55921 gaagagaaag ggtttatgag ttttcaattt tgcaggcgga agttttattt cctcctggcg
    55981 ctggccttac cgggctacgc cgccgtccat cccgtgcagc acagcgcgcg ggaacaagtc
    56041 aacgcccagg tactgaatgc cgccagccaa aaaattgaat cgctggcgca acagcggcgc
    56101 tggcatgact atcgctacac ctttaaggtc tacattccct cgcagattgc gaccgccgcg
    56161 ccctgtacta aaacacccgg cgttacgctg acttcgcctg gggacatcgc cctcaaccgg
    56221 atgaacttca ccgtcagttg cccacaaagc tggcagatga acgtagcggt gcgtccggat
    56281 gtactggtgc cagtagtaat ggcgaagtca ctggttgccc gcgatacgcc gctcaccgcg
    56341 aatgatgtgg agttgaagcc ttacaacgtc agtgcccagc gccgggaggt gttgatggaa
    56401 cccaatgacg cgattggttt cagcagtaaa cacgcgctgc aaccgggcag gccaatcacc
    56461 aaagaagaac tgatatctcc ggtgctggtc gagcgcgatc aaccggtgat gattgtttac
    56521 cagagcgcgg ggatcaccgc ctcaatgccc ggcgtagcac tgaaaaatgg tcgcaaaggg
    56581 gaaacggtga aggtgcgtaa cgccagcagc cagcgggtca tcagcgcgat ggtggcggaa
    56641 agcggcgtgg tgacaacggt aagcgcggag taatttgcgc gggcgattaa agttttgccg
    56701 cctgttaacc gctttaccgg agtaacccac ccaaaatacc gagacgaggt tacgatgaaa
    56761 atcaccccga ctatgccagg caatcacccg acagccacaa tggcaggctc cagtcagccg
    56821 cgcaaaagca cggcgaccac cgccacgacg ctgagcgccg atgacattac ccaggcggga
    56881 ttgcagtctg cccagcaaac gctgaacgac cagcaagaga gcgatatcga cagcgacaaa
    56941 gtggcacaaa tgcaggcgat gctagcctcc ggcagcctgc aagtggacac cgagcagtta
    57001 gccagcgaca tgctgagttt tttccagaac taagaggccg atgaagtgag tatccggtta
    57061 caacaggtga aggcgttgtt gcagggaatt cgtgacgacg acgcactgta cgattccctg
    57121 cgtgaacttc tccagcgtca gcggatctgc atgattcgcc gcgccagaga ggatctgctg
    57181 gcggtaaacg acgagataac gcagcactat gagcagttgc atgcgcatag ccatcagcgg
    57241 catagcctgc tgaaaatgct cggtgttagc gtaaatcgtg acggtctggc gcaggttttt
    57301 gcctggcttc cggcggtaca aaaagcggca gcgcaacagt tgtggcaacg ccttgagcaa
    57361 aaagcagaac gctgcaaagc ctataacgat aaaaacggcg agctgttaat tcgccagtat
    57421 gaatttattc aggctttttt aggcagcgaa gcggattttc tctatcaggg ataacgacaa
    57481 ttccttcccc catcgcggaa acggattttc cgcctgatcc ccaatccttt gtttttgatt
    57541 aaaaaaattc ccggcacgct tattgcacat cctgcaaggt aataaagact ggaaactacc
    57601 tatgagtttt attgaatgtt acgaaccgga atatgtgcgc aattttatgg cacgccaccc
    57661 cgattcgcct ttatacgtcc gcaaggtgtg gaaaaacgag attcgccaga gcctgatgct
    57721 gacagagcca gaaagctgcg aagcggtgct caacgatgcc tgcgcctttg atttacagct
    57781 cacctaccgc ccggtggaag aaaatgccgc agaactctcc gcgcgcgatg cgatagtaaa
    57841 ccaggccatt ttgagcaccc ttacgctccc cgatttaacc ccggagcttt acctgtatgc
    57901 cgtcggaatt gtgctctccc gcgccagtaa aatgccgggc agagatggag atattcttgc
    57961 ccgcttgacg acgctgccac aggcgctggc cgatcatgca caaaaaggca cgctacaggc
    58021 acagttcgcc cagctcccgc ccgtgccaca gccggcgcgc cagttgatga cgctgctcgg
    58081 cagttgtgca tttgactggt cgattttgcc ggaaagcccg cgcaaaactt ccctgccgtt
    58141 gcaaatgcct ttgctgacgc tacatgacgc caacagcaaa gccctgttac aacagcagct
    58201 taaggtgcag tggcaaacca cctggcaaca gcattttgcc accgcgccgt ggatgatgcg
    58261 taactggctg atttaccgcg tttaccatga ggtgattgga caggcagacg gcgcagatta
    58321 ttgcccgctg gtgtgcgatt tttatcttat ccgcacgtta atcagtttat ggacgctgga
    58381 tggttctccg ctgcgacagg aggatatttt cgccctcttc gccatgtttg aacgctggcg
    58441 agagagtgaa aatgccgtgc tgattcgcca acaaattcag gctgaacgtt ccgcagatcc
    58501 cctgctttat gcgttttcac tgctgtcttg ctaacgcttt ctggccggtt ttcccggcct
    58561 tatcgacttc gtaaactatc atgcaaaccc tcatcaaaac actcgtcact caccgcgtaa
    58621 aaccgtttgt cagcgtcgtc acgccaacct ggaaccgcgg cgcattcctg ccgtacctgc
    58681 tttatatgta ccgctatcag gactacccgg cagaccgccg cgaactcatc atcctcgacg
    58741 actccccgca aagccatcaa cacatcatcg accgcctgac caatggcacg ccggaatcct
    58801 tcaacatccg ctacatccat cacccggaaa aactgccgct gggcaaaaaa cgcaatatgc
    58861 tcaacgaact ggcgcgcggc gaatacatcc tctgcatgga cgatgacgac tactactcgc
    58921 cagataaaat ctcttactcc atcgccatga tgcaaaaaca ccgggcgctc atctccggtt
    58981 ccgaccagat ccccatctgg tacagccaca tcaaccgcat tttcaaaagc cgcagctttg
    59041 gcccacataa catcctcaat ggcacctttt gctatcaccg taactacctg aaaaagcacc
    59101 gttacgacga cgattgtaat ttaggcgagg agaaaagttt caccgacaat ttcagcgtta
    59161 acccgctgca actgcccggc gagcgcacga ttctgtgtat ctcgcacagc cacaacacct
    59221 ttgataaaga ctttatcctc ggggccagca cgcctgtaaa cgcggtgctt acggatatcg
    59281 tcagcgatcc gctgctacgc aacgcttatc tgggtctgca taacgccact cattatcagg
    59341 cgatccacca tcaggcgatc gatcaggtcg tattgatcaa tctggataaa cgcccggatc
    59401 gcttacagca gatccgtgaa gaactgacat tgctgcatat tccgcctgag aagatcaccc
    59461 gccttgccgc cagtgaaaat gaaaacggtc aacggggccg ccagcaatca cacttgcagg
    59521 cgctgcgtct ggcgcaacag cacggctggc aaaactacct gttgctggaa gatgacgcgg
    59581 ttattctcaa acaggagaaa catattcagg tgctgaacgc gctgctggcg agtctggcga
    59641 aaattccctg gcaggtgatg atcctcggcg gcgaaatttc gcagggaacg atgctaaaaa
    59701 gtttgcctgg gctggtgcac gcgcgggact gccgcaaagt gtgcgcttat ctggtgaaca
    59761 gccgctacta cccgcagctg gcgcagcaga tgaacaacga tgagcattcg ctggaagacg
    59821 gctggcagcc gctgctgcac accgacaaat ggctggcctg ttacccgagc ctttgttatc
    59881 agcgacaggg ctttagtgat atcgagaaaa aagcgactga taacattaac cactacttca
    59941 ataagttacc cgtagccacc aagccatcta cattacctat cgccgatacc atcggctttt
    60001 ttatggaaac ctcattccac tacgcgctct accgcccaat catcaccgcc ctgcaggcgc
    60061 aggggcagag ctgtacgctg gtgatcaatg accgcgtatt taaaccattt ctggatgaaa
    60121 tgctggcgac gctaaaaagc atcgacgatc cgcagctcaa aggaatgcgc ttatctgaaa
    60181 tgcaggcaca cggtcagcga gtgaaatgcc tggtcagccc ctatcacacg cccgcgctca
    60241 atggcctttc aaccctgaac attcgcgcca tgtacggctt agccaaagaa acgtggaacc
    60301 acgccgactg gaaccgtttt tatcagcgca ttttgtgtta cagccactac agccagcggg
    60361 cgctggcaca tttcggcagc gcaaaagtgg tcggcaatcc gcgcttcgac gcctggcata
    60421 acggcacgtt tgaccgcgct ctgccggaaa atatccaacc cgattcccat aaacctacgg
    60481 tgctctacgc ccccacgttt ggcgcattaa gctccctgcc ccactgggca gaaaaattgg
    60541 gccgcttaag cggtgatgtg aacctgatgt gcaaactgca ccacggcact tgctcgcgcc
    60601 cggaagaagc tgcatcgctg gcactggcgc gcaggcattt aaaacagcgc accgactctg
    60661 cccgccacac gctggcgcta ctggcaaagg ccgattacgt gctgaccgat aacagcggct
    60721 ttattttcga tgctattcac gttgataagc gggtgatcct gctcgacttt ccgggcatga
    60781 caacgttgct cgacggtgag aaaagttatt ccacgcccga aagtgccgac cagcaaattc
    60841 gcgaaatatt gccggtggct catgatgttg cggaattacg ttatctgcta tcagaggcgt
    60901 ttgactggtg tgcggtacag gggcaactgg cggaaattcg tcatcactat tgcgatgcgt
    60961 ttatggatgg caaggcggga gaacgggcgg cgatggtgat tatggaggcg ctggggtaaa
    61021 ccgcgcaagt gcctgatgcg cttcgcttat caggcctacg cgcccctgca atatagtgaa
    61081 tttgcgatga tttgtcggcc ggaaaaggcg tttacgccgc atccggcacg aacagtgcac
    61141 ttatctaccc ttcgatttca aatacatcgc tttatggctg gttaatcggt aaaagttgca
    61201 cattttgata gcgagaagag ctgtctttat gaattccgaa aagcgccctt ctaccctcta
    61261 taaccattct tttttacctg tattggccgc aacatacctc acccacgaag tggttgtagc
    61321 aattcgtggg ctaaaggtcc gcattatatg aaacgggagg gcggtagcat ttctgatatt
    61381 acatgcctca attttgctga actattggct atcctggacc tcaaaaatct ggtcataaga
    61441 tagatttttt aagggaatac ggagtaaagg tgtggaaaac taattaatag gtttgtctta
    61501 atgcttcaga agattggata catggaagaa gataatatta tagagaaaaa accatggcgt
    61561 gggatcccac ctgaatacac ggatagatgg agggaaattt ataacaacgc tgctcccgaa
    61621 gaagggatgc atttatcaga tgcttgtcca gtatgtggtg atacggaatt atatcgctat
    61681 tattctctgt taaaaacgca accacgctta ttaggaggga ttatgtataa aggacctgga
    61741 agttattggg aatggtgtgg gtcttgtcgt tcctatgaac atatgtcagg ctatgtacca
    61801 atatcgtggg ttgtggagtt atcaggaata gatcattcaa aattaacggc cattccagat
    61861 atgattgata ttgcaagaca taaaaatcat tacttctctt aaatattatc taattagttt
    61921 tcccggacag ggcaagttgg cgaaaatagt gaggagcatc acgttttcca tcctgactga
    61981 cacgcctact ggtgattaaa atcaaaccta tggaatgacg aaaaagatgg aaaggaaaag
    62041 catatcaagt aatcagtcag gaagatattc acaacaatca cctgaatcag tagatcgaat
    62101 gaaggatcac ttgtcacata atataattgt cgatatagat cttaattcac caggagttgt
    62161 tactaaactc gatttattat taagaaaaaa taaaatatca aaaacatgtc tctgcgggat
    62221 gtttccagta tctgccctga acagaaagac gttaccagta tacttgtcgg gctatgggct
    62281 ttagattttt tataatcatc accaaacata tatgacaaaa taaaaacaaa tgaatttctt
    62341 ctctcaaaca gtgatattaa aacaatatca acaggattag ttgatataat acggcattct
    62401 aattttgaag cttcagaaga tgataatgca atagagtacc attatgctgc atgtttgagg
    62461 ctttatagat tgcttggtga tgaagcagag gatatattgg ttgaggaatt attacaacat
    62521 ccaacaataa ccatattaag ggcaatcggt tttttagttg gacatgagat aaaatgaacc
    62581 attaataaaa atacattttg tatacttaat gaaatctctg aagcatattc cgagcaatta
    62641 tctattgtcg caaaagacgt attaattgaa gatgttgcgc gagttaatgc aataaatcaa
    62701 tttctataaa tttacgatat ttattagata attatgccga gtattcaatt tggagatgat
    62761 caggcagact atccgttttt atcggaggga gtcatctggc taaggttgat gtgatatgtc
    62821 cgttttatcg tcaaggttaa acggaatata agaaatgacc acctcgcttc agtccttata
    62881 gagagcgact gtcgtaacct tctcccaagt ttaaagagaa ccagatatga aaacttatca
    62941 tctaagttca gcaaacagaa gtgcagaaca ttatataatg ttctcatatg gtgtatcatt
    63001 acgactatct aattaccttt ttaacagtaa accggttgca agagattttt ttaaacattt
    63061 aaaaggaact acgcagcgag gtttaacaga taagcattta gacaatttag attacttcaa
    63121 gggcttagga gcttatcaca ttgtatctaa acgcttcgtc gaagcaatga aatcatattt
    63181 aaaggaagat gtaacttttc atgcaatgca agtctcagga aggaatactt ctcacgactt
    63241 atacctttta caagtcaata aaactttgcc aataattaaa gaaacatctt tcgatttaaa
    63301 caatgatcgt gtattttcgc cacccatttt caaaaaaaat catgacatat catttctaat
    63361 agccagagaa gagaatgtct taatgaatca tattgtcatc gtcagcgatg aatttgtttc
    63421 tttgtgtgca cgacatcaat taaaaataaa cttccgacaa acacttacgg aataaattaa
    63481 acttaagaca tccatcaata ttattgttag tatacattcg atctcagcgt atttattaac
    63541 aaaaacgaca ggaaatattc atatgaaaaa agtttatttt gttcatcgtg ataagaatgc
    63601 aattgaacgt caaagtgacg gtgtagaatt ttgtttcatc cctgagttca atgacggaag
    63661 aatttatttc tattgtcatg aatatgatat attttggcgt tcaatcaagg atgctggtga
    63721 ctatgcctgg tgctgtaatt tccatctaaa gggaattata cgtccggcaa ctcttattga
    63781 gatatcaaat tccgatttaa tatcttatat tgactctatt aaagaatatg aaatagagaa
    63841 cagtaaactc ataaacatta actatattca tttgaattat gattttttaa atattcatca
    63901 aaatacctaa aatagaggaa ctcatattat cagaaatgcc ccctaatcac ctcaatgatc
    63961 cccgtcgtcg ataccgacgg cgtgcgggga aagtagacca cctcacatac acacgagaaa
    64021 tcgtcaaact tacctgccca gtcatcgccc atcaccaaaa tatccgcact gtacccgcgc
    64081 aaatattccg ctttctgctc cagcgactcc tccagaaata cgccatccac gcacgccagc
    64141 ccggcgataa tcgccatgcg gtcatcctga gggtaaaccg gagccctgcc ctttttggcg
    64201 atatttaagg catcggatga tacgccgacc agcagctgct cgcccagcgt gcgagcgcgt
    64261 tgtaaaaggc gcagatgacc cacatgaaac acatcgaagg tgccgaaggt gatgaccgtt
    64321 ttcatggcta ctccgctaat ttgtgccgca accagcactg gacggcgatg tcatccaccg
    64381 ctttttgcgc tttcgccgtg gcgctttcct gttgctcgcg gcgtttgtcg tccagcgtca
    64441 gggctaccac tttctcttcg cacgccgccg ccgtcaggtt tttctgcatt cgcgtagctt
    64501 tgatgttggc cagcgttttt tcctgctctt gccaggcgat caccttgcgc agtgtgcctt
    64561 tgtagccgct gacgtttttc agcgactcca ccgacgccgc cgcgcccgca gatgttttct
    64621 cgacaaggta acctaatgct ttgatattgt tatcatagcc agcgcaaatc tgtttttgct
    64681 ttgccagctc gacggttttg tctttgaccg atttatcccg caggcgctgc aactgcgcca
    64741 gggtgtcgat gatttgtcgc atggttattg cccttctgcc tttttggcga tgggaaagag
    64801 gatttgcagg cggctttgca ccagttcgag ggaggcgggt tcgcgcatct cctggcgcaa
    64861 aaatcgctcg atagccggga acattttcac cgctttgtcg acgctggcgt ccgcgcctgc
    64921 cacgtagccg ccgagcggga tcagcggttt gatctccata taggcggcgt agttttgctt
    64981 taacgcgcgg gcggattgct gatgttccag gcgggtgacc tggttcatgc agcggctgat
    65041 cgactggccg atatcaatag ccggatagtg ccccgcttcc gccagtttgc gggttaatac
    65101 gatatgtccg tccagcactg cgcgggcgca gtcgactatt ggatcttgct ggtcgtcgcc
    65161 ttccgccagc accgtgtaga tggcggtcat cgatcctgag ctttcgctgt tgcctgcgct
    65221 ctcgaccagt ttggggatca tgccgaatgc cgatggtgga tagcctttgg tggcgggtgg
    65281 ttcaccgagt gagagagcaa tttcacgctg cgccatggcg tagcgggtca gggaatccac
    65341 cagcagtaaa acgtgatgcc cgcggtcacg aaaccaggcg gcgatggagt ggcacagttc
    65401 ggtggctttc aggcgcatca gcggcgattc atcggcaggc gcgacaacga cgatagactt
    65461 tgctagcccg tctgcgccca gcgagtggtc gataaactct ttcacttcgc gcccgcgttc
    65521 accaatcaac ccgaccacca cgatgtcggc tttagtctgg cgggtgatca tccccagcag
    65581 cacgcttttc ccgacgccgc tgcccgccat cagcccgaca cgctggcctt tgccgatggt
    65641 cagcaggccg ttaatcgctt tcacgcccac gtccagcggt tcgtccactg aacggcgggt
    65701 taagggattg accgagggtg gcagcggcgg cagaaggtcg ttgccgttca ggcggccttt
    65761 gccatccagc ggttcgccga gaccgttaac cacgcggcct aaccagcttt cgccaatcag
    65821 gatgtcgtgg gctttttctt ccgggaagac gcgtgcgcct gccatcaggc cgaccggctg
    65881 tttaaacggc attaagtagg tgatgtcgcg attaaagccc actgcctgcg cgtcgatcag
    65941 tgtgtgattg gcgctttcga tgcggcacag ttgcccggtc attagcgggc agccgacgct
    66001 ctccagcaga atgccgttca ggcgcaccag ccgcccggcg acgcgggcca gcgggatcga
    66061 ctccagcgag cgcagggcgt tatcgaaagc agaaaaatca ctcaccctgc gactccggcg
    66121 ttagcgtctc ttgcagcgcg gtcatgcact gttcaaggcg atgctcacag ccgatgtcca
    66181 gttcagattt gtcggtaatc acccggcatt cgcctggcgg cagatccggc gaaggcgtca
    66241 gcccccattc gctgactttt tccggcaccg cgtcgcgcag gcggttaaac tcttctgtac
    66301 tcagcatcac ttgcagggtc tccggcatcg tcgggaaggc cgccagcgcc tcttccacca
    66361 gcgccagtaa ctgcgtcggt tgcagcgcca gttcgcagcg gatcacctga cgggtgactt
    66421 tctccaccag ttgcagcaaa tcttcccgct gtttacgttc aatatgcgcg aggaagtcgt
    66481 tcactttacc ggtgatggct tccagcggct gcgccgcctc aacaaactgt ttgcgtcctt
    66541 cctgctgccc ggcgaggctg ccttcggtgt agccctgacg ccgcccctcg gcaaaacctt
    66601 gttgatgacc ttcgctaaag ccctgctcct gaccttcggt cattccctga gcaaaccctt
    66661 tttgcagccc ttcctgaaaa ccgtccatca actgacgctg gtaatcggcg gcgctgatac
    66721 tggcagtaag cggttctccg gtcaccgcac gctggcgcgg cgggaagcgg tgcaggcgat
    66781 agcgcccgcg cagtgtttca atggtcatga attactccgc cgtccgttcc gcaaagagct
    66841 gtagctgaat ttcgcccgct tcttccagct cacgggcgat gcccatgatt tcgcggcgga
    66901 tctgttcgat acggctaacc ggcaccggac ccagacgcgc ggtgatcgct tccagttgct
    66961 gcgcctgacg ttttggcatt accgcgtaaa tcgagcggcg cagcagggct tcggtgcctt
    67021 tcagcgccac cgcccagtct tccatcggca cttcatcaag cagacgccga cgcacttctt
    67081 cgttctggcg gctgaggata aagaagtcgt acatctcatc ctggatctgc cccaacacct
    67141 cttcgtcgcg ttcgcgcatc tggtcgagga tcacctgctg attgccctgg aagcggttga
    67201 cgatatccgc cgcctgtttg atgcctttta ccttcgagcc atgttccgaa agtaccgaca
    67261 ggccacgttc aatcaggcgg tccagctcat ccaccacgtc gcggttgacg tcgttgagtt
    67321 tcgccacccg gtagagaatt tcgttttgca ccgattcgtt gagataagag agcaccgctg
    67381 ccgagatttc cggcgtcaga aacgcgagga acaccgcctg caattgcaga tgttcttcgg
    67441 aaatcagcat cgccaactgg cgtggttcca cccattgcag gcgcgccatc cgcgagcgaa
    67501 tctcatcgcc atagatgccg ttgatcacgc tgctggcgat ctcggtaccc aacgccttgt
    67561 taagaatgcc ctgcaacatg gatcgcgatg cgccgttgat cccgctctgt tcgcggaact
    67621 cgtcaaagaa gtggttgatc actttacgcg ccatgctggt tttgacgccg gaaagtcgcg
    67681 ccatgttttc gctcaggcgc accacttctt cgcggctgag tttttgcatc accgtcgccg
    67741 ctgcctcttc gcccagacac agcagcagga tcgccgcctg ttccaggtag ctgttgctct
    67801 tatttgttgt caattcgctc attgctggtg atccattgtc tgagaacttc ggctacgcga
    67861 tcggtgtcgc tcatggccag tttttgcagg aactcgagtt tcacttcgag gccagaactt
    67921 tgtgacggca ggctgtcatc gccgggcagc gacggcaggt cgacgttttt gcgctcctct
    67981 tccggcatgg caatccacgg ttcgctgacg gcttccggtt ctggtgtgat aaccggcgca
    68041 cgtaccgccg tcagccgttt catcaacgga cgcaccacga agaacagcag caacagcgcc
    68101 agcaggccgc agccaatcag ccgcacccag gccagtacgc tgtcatcttt ccacagtgga
    68161 ataaccggtt cgacaggcac cgcctgcggt acaaagttga gtaacgataa ggagagattg
    68221 tcaccacgct gggcgtcgat cccggcggca ttgttcagca acgcggtcag ttgcgtggtt
    68281 tgctccggct tccagttttt cagcgccgga gcgttttgat tgagtaccac cgccacgttg
    68341 aggcgtttca ccgcgaagcc gggatgctga atatgctgga cgctgcggtc gtaagagtag
    68401 tcgcgcttac tttcgctgtg tttggaaagc gcttccggcg agcggttttc ttccgtaccg
    68461 ttggtcatct gattcgctgc aatcggcgga cggttactga gcgaaccagg cacgcccatc
    68521 gccacctgat tggtgtcgct gtccagcacg ctctcttcgc ggttgatttt cggcgcgtca
    68581 ccgtagtgtt cctgggtttc gtcgatggtg ctgaggtcga gatccggcat cacactgacg
    68641 cggtaattcc ccgtgccaac cagcgaatcc agcacgttcg ccacgctggc acgggttttg
    68701 tcctgaatat ctttgaggat ctgatcgcgt ttacgggtcg cggcagaaac cgcctcgcct
    68761 gcgccaatgc cgtcggtcag cagatttccc gcctgatcga cgacgctaac tttgctggcc
    68821 tgtagccccg gaatactgcc ggacaccaga tgcacaatgg cgttcacctg gtccatattc
    68881 agcttcgcgc cgtaatgcaa gcgcaccacc accgaggcgc tgttttgcgg ctcatcgctg
    68941 accacaaacg agctttcttc gttgagcgcc agatgcaccc gcgcgctttc caccgcgtcc
    69001 agcgacatga tgctctgcgc cagctcccct tcgagactgc gtttatagcg cacgttctgc
    69061 acgaactgac tgctgccgag cacttcgtct ttatccatca gctcgtagcc gctgggcaat
    69121 atcgcctgca cgcctttcgc ggcaagggtc attcgcgttt tcgacagtgc atcttccggc
    69181 accagaatct gcccgctctg cgggtcgatg cggtaggtga gcttttcgcc gtccagcacc
    69241 gtgacaatct gcgacacggg cagattttcc tggcgtccgt agagcgaaac gtagccgcga
    69301 ttaccggtcc acagcacgct gacgataatc gccgtcaccg ccacggcaag ccccactccc
    69361 gccatcaaca cccagcgttt gttatctacg agatgcagtc gaaacgccgg aaacgcctgg
    69421 gttaacttct ttatttgtgc attcataggg ttagatcgac atactcatca ggtcgttaaa
    69481 cccggtggcg attttattgc gcacctgcac cagcgcggag aaagagagcg acgcctgctg
    69541 gctggcgatc atcgcacctg ccaggtcatc acttttgccc gtgtcgacag ccgtttgctg
    69601 ctgctccgcc acctgctgga actgatcaac gtgaccaagc gcgccgtaca taatgcggtt
    69661 aaacgacacg tccgggtctg tggaactgaa ggtcatggag ggtagcaccg gcgcctgggc
    69721 gttggcctgc atccgctgca cgtcctgcat gatttgcgcc tgcatggtgc tgttgattgt
    69781 ggtaatgctc atttttaact tccgggtcag gagaaaactt cgataccttg cttgcgcatg
    69841 gaggccagcc gatagcgcag tgcgcgcggg gtaatgccta acaggtcggc gattttgctg
    69901 cggttgccct gatatttacg catcaggtcg gcgatatatt gatactgcgc acttcgtccg
    69961 tgctggccta aatcgcgggt ttcggcaatg tggacggcgg gctgaacagg ccattgcagt
    70021 tcgctgtggt cggcaatgtc agtttccggc aggcctaagg catccgggta aatcacgccg
    70081 tcgcggttta agatcattcc tctctgaatg gcgttttcca gttggcgaac atttccgggc
    70141 cagcggtaat taagtagcgc ccggcgtgtg gattcggaca gggtgatatt tttcaccagc
    70201 accgtggcat attttttaat aaacgactct gccagcggaa taatatcgtt cagacgttca
    70261 cgcagtggcg gcatggttat aggaataacc gccagacgat aataaagatc ttcacgaaaa
    70321 cgtccagcag ccacgtcctg ttccagattt ttattggtgc aggcaatcag gcgaaaattg
    70381 agtttgattt gtctattgct gcctaatcgc tcaacctgct gttcctgcaa tacgcgtaat
    70441 attttggcct gtaatgccag cggcatatcg ccaatttcat cgagcaataa ggtgccgtta
    70501 ttagccagtt ccatttttcc tggcacgctg gcaattgcgc cggtaaatgc gcctttgtcg
    70561 tagccaaata aggtcgcttc cagcatattt tccgggatcg ccgcacagtt cacgccgata
    70621 tagggcgcgt tatcattttc accaaaggct acggtgtgaa tatatttcgc cacgcactct
    70681 ttccccgcgc cggtttcgcc ctgaatgagc accggtacgt taaaggcggc gacacgcttt
    70741 gccagagtaa aagcgttaat gctggaagcg gcagttgcaa taagttcaga catagggtga
    70801 acgatataag tagcgacaga aaacaggatg ttctgaagcg tatcaccggt gggattaaca
    70861 agttcgaatt aaacgataat aaaaatatta tttgtttata aacaactagt tagcaatgta
    70921 ggccggataa ggcgtttacg ccgcatccgg caattgcgtg ccgatgcctg atgcgacgct
    70981 gcgcgtctta tcaggcctac agtttgtgca atgttttatg acgatgcccg atatctcgcc
    71041 cgttaataat ttaattattt tttcacccta ttaataattt gcccctttca ctccc
//
LOCUS       DAOFSV010000007        67127 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.007, whole genome shotgun sequence.
ACCESSION   DAOFSV010000007 DAOFSV010000000
VERSION     DAOFSV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.007"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(13..2082)
                     /gene="glyS"
                     /locus_tag="QBH27_000533"
     CDS             complement(13..2082)
                     /gene="glyS"
                     /locus_tag="QBH27_000533"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDQ7012897.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2092..3003)
                     /gene="glyQ"
                     /locus_tag="QBH27_000534"
     CDS             complement(2092..3003)
                     /gene="glyQ"
                     /locus_tag="QBH27_000534"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDQ7012898.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3098..3397)
                     /locus_tag="QBH27_000535"
     CDS             complement(3098..3397)
                     /locus_tag="QBH27_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDQ7012899.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3572..4567
                     /gene="wecH"
                     /locus_tag="QBH27_000536"
     CDS             3572..4567
                     /gene="wecH"
                     /locus_tag="QBH27_000536"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDQ7012900.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVINAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            complement(4609..5046)
                     /locus_tag="QBH27_000537"
     CDS             complement(4609..5046)
                     /locus_tag="QBH27_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDQ7012901.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5092..5433)
                     /locus_tag="QBH27_000538"
     CDS             complement(5092..5433)
                     /locus_tag="QBH27_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012902.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5602..7056)
                     /gene="xylB"
                     /locus_tag="QBH27_000539"
     CDS             complement(5602..7056)
                     /gene="xylB"
                     /locus_tag="QBH27_000539"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDQ7012903.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7127..8449)
                     /gene="xylA"
                     /locus_tag="QBH27_000540"
     CDS             complement(7127..8449)
                     /gene="xylA"
                     /locus_tag="QBH27_000540"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDQ7012904.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8815..9807
                     /gene="xylF"
                     /locus_tag="QBH27_000541"
     CDS             8815..9807
                     /gene="xylF"
                     /locus_tag="QBH27_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7012905.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9885..11426
                     /gene="xylG"
                     /locus_tag="QBH27_000542"
     CDS             9885..11426
                     /gene="xylG"
                     /locus_tag="QBH27_000542"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDQ7012906.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11404..12585
                     /gene="xylH"
                     /locus_tag="QBH27_000543"
     CDS             11404..12585
                     /gene="xylH"
                     /locus_tag="QBH27_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDQ7012907.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12663..13841
                     /gene="xylR"
                     /locus_tag="QBH27_000544"
     CDS             12663..13841
                     /gene="xylR"
                     /locus_tag="QBH27_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDQ7012908.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13949..14773)
                     /locus_tag="QBH27_000545"
     CDS             complement(13949..14773)
                     /locus_tag="QBH27_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDQ7012909.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15093..17123
                     /gene="malS"
                     /locus_tag="QBH27_000546"
     CDS             15093..17123
                     /gene="malS"
                     /locus_tag="QBH27_000546"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDQ7012910.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWNDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKIIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALHEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17301..18554
                     /gene="avtA"
                     /locus_tag="QBH27_000547"
     CDS             17301..18554
                     /gene="avtA"
                     /locus_tag="QBH27_000547"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDQ7012911.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18593..19066)
                     /locus_tag="QBH27_000548"
     CDS             complement(18593..19066)
                     /locus_tag="QBH27_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDQ7012912.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19168..20016)
                     /gene="yiaJ"
                     /locus_tag="QBH27_000549"
     CDS             complement(19168..20016)
                     /gene="yiaJ"
                     /locus_tag="QBH27_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDQ7012913.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20217..21215
                     /gene="yiaK"
                     /locus_tag="QBH27_000550"
     CDS             20217..21215
                     /gene="yiaK"
                     /locus_tag="QBH27_000550"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDQ7012914.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMCGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21227..21691
                     /locus_tag="QBH27_000551"
     CDS             21227..21691
                     /locus_tag="QBH27_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ7012915.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALDPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            21714..22634
                     /locus_tag="QBH27_000552"
     CDS             21714..22634
                     /locus_tag="QBH27_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HDQ7012916.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            22748..23215
                     /gene="yiaM"
                     /locus_tag="QBH27_000553"
     CDS             22748..23215
                     /gene="yiaM"
                     /locus_tag="QBH27_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDQ7012917.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            23218..24495
                     /gene="yiaN"
                     /locus_tag="QBH27_000554"
     CDS             23218..24495
                     /gene="yiaN"
                     /locus_tag="QBH27_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDQ7012918.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            24508..25494
                     /gene="yiaO"
                     /locus_tag="QBH27_000555"
     CDS             24508..25494
                     /gene="yiaO"
                     /locus_tag="QBH27_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDQ7012919.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRQPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            25498..26994
                     /gene="lyxK"
                     /locus_tag="QBH27_000556"
     CDS             25498..26994
                     /gene="lyxK"
                     /locus_tag="QBH27_000556"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDQ7012920.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRSLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GALFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTRVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            26991..27653
                     /gene="ulaD"
                     /locus_tag="QBH27_000557"
     CDS             26991..27653
                     /gene="ulaD"
                     /locus_tag="QBH27_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDQ7012921.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            27646..28506
                     /locus_tag="QBH27_000558"
     CDS             27646..28506
                     /locus_tag="QBH27_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDQ7012922.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMLETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28500..29195
                     /gene="araD"
                     /locus_tag="QBH27_000559"
     CDS             28500..29195
                     /gene="araD"
                     /locus_tag="QBH27_000559"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDQ7012923.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(29227..30042)
                     /locus_tag="QBH27_000560"
     CDS             complement(29227..30042)
                     /locus_tag="QBH27_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7012924.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEDERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            30268..31668
                     /locus_tag="QBH27_000561"
     CDS             30268..31668
                     /locus_tag="QBH27_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7012925.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSSPA
                     YQTLWLVVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            31679..33652
                     /locus_tag="QBH27_000562"
     CDS             31679..33652
                     /locus_tag="QBH27_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDQ7012926.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQVDN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEETSAIRR"
     gene            complement(33829..35367)
                     /gene="aldB"
                     /locus_tag="QBH27_000563"
     CDS             complement(33829..35367)
                     /gene="aldB"
                     /locus_tag="QBH27_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDQ7012927.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSYGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(35475..36770)
                     /locus_tag="QBH27_000564"
     CDS             complement(35475..36770)
                     /locus_tag="QBH27_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDQ7012928.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(36900..38051)
                     /gene="yiaY"
                     /locus_tag="QBH27_000565"
     CDS             complement(36900..38051)
                     /gene="yiaY"
                     /locus_tag="QBH27_000565"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDQ7012929.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(38242..40086)
                     /gene="selB"
                     /locus_tag="QBH27_000566"
     CDS             complement(38242..40086)
                     /gene="selB"
                     /locus_tag="QBH27_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDQ7012930.1"
                     /translation="MIITTAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(40083..41474)
                     /gene="selA"
                     /locus_tag="QBH27_000567"
     CDS             complement(40083..41474)
                     /gene="selA"
                     /locus_tag="QBH27_000567"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDQ7012931.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQALLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(41572..42180)
                     /locus_tag="QBH27_000568"
     CDS             complement(41572..42180)
                     /locus_tag="QBH27_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDQ7012932.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(42251..43387)
                     /locus_tag="QBH27_000569"
     CDS             complement(42251..43387)
                     /locus_tag="QBH27_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ7012933.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLEPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(43390..43752)
                     /locus_tag="QBH27_000570"
     CDS             complement(43390..43752)
                     /locus_tag="QBH27_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDQ7012934.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            44289..46202
                     /gene="mtlA"
                     /locus_tag="QBH27_000571"
     CDS             44289..46202
                     /gene="mtlA"
                     /locus_tag="QBH27_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDQ7012935.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            46522..47670
                     /gene="mtlD"
                     /locus_tag="QBH27_000572"
     CDS             46522..47670
                     /gene="mtlD"
                     /locus_tag="QBH27_000572"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDQ7012936.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            47670..48257
                     /gene="mtlR"
                     /locus_tag="QBH27_000573"
     CDS             47670..48257
                     /gene="mtlR"
                     /locus_tag="QBH27_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDQ7012937.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(48268..48477)
                     /locus_tag="QBH27_000574"
     CDS             complement(48268..48477)
                     /locus_tag="QBH27_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012938.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            48761..49123
                     /locus_tag="QBH27_000575"
     CDS             48761..49123
                     /locus_tag="QBH27_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDQ7012939.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            49667..50347
                     /locus_tag="QBH27_000576"
     CDS             49667..50347
                     /locus_tag="QBH27_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDQ7012940.1"
                     /translation="MSMKTLLNVLTIFSLGTLTGCDYIEKASLIDDLAKQQELQKSKI
                     EELEKQQEQYKNKAELFEKQQNNITNSTRILASIVKTVKEKQDEFVFTEFNPLQTQYF
                     ILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNEKPTDAKALA
                     KWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPAE
                     TRQRKNKK"
     gene            50391..54734
                     /locus_tag="QBH27_000577"
     CDS             50391..54734
                     /locus_tag="QBH27_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDQ7012941.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYEGQPVDYGNGSAGDGWVAIGKNSKANTFVNGSGASTALGYDAIAEG
                     EYSSAIGSRTHAIGGASMAFGVSAQAIGDRSVALGASSVATGGRSMALGRYAKTNGFT
                     SLAIGDTALADGEKTIALGNTAQAYEIMSIALGDHANASKEYSMALGASSVASAANAI
                     AVGRDSAANGTDSLALGRLSLASAANAIAMGAESEAAENATAIGFNADAIGKSSLALG
                     DNASAGETNSIAIGQGSEASKLDSIALGSNSRSAGENAIALGNNSNAGGKNSLAFGFN
                     TTANGDNAVAIGANSSAGADNTVSVGSSSLKRKIVNMGNGDINNVSSDAINGSQLYAI
                     SKSVSDRLGGYHDDPDNVINSDGTLKAPTYYLQSGQYNNVGEALQSIDNNTLHWDSKS
                     NKYSASHTVFNANGSVKSTSAKNIITDVADGTISATSSDAVNGSQLYNLKQDALLWDG
                     TAFSAKHGTSNTNSKITNVADGAVSASSKDAVNGSQLYDLKQDALLWDGTAFSAKHGT
                     SNTDSKITNVLAGTVSSTSTDAINGTQLQSTASTITSYLGGGAAMSNDGVFTGPQYNI
                     GGKNYTNVGAALTALDNDALLWDATANSNTGAFSAAHGTLKTASKITNVANGDILANS
                     YDAINGSQLYSLADSFTSYLGGGADINNAGVLIAPTYTIGSKTYNNVGDALNAINSSF
                     STSISDALLWDSTANKFSAKHGGTNSIITNVADGTISSTSSDAVNGSQLYDTSKYIAD
                     ALGGDAEVNADGTMTAPTYSIANEDYHSVGEALDALDDNALLWDEDAGAYNASHDGNA
                     SKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGIN
                     YVRTNDNGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSSSSVDTGIALGSSSVS
                     SRVIVKGSRNTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVT
                     VRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLV
                     LADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSE
                     DGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENG
                     IGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTI
                     SVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGN
                     GGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIA
                     SAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGE
                     YSAALGAGIQW"
     gene            55152..56807
                     /gene="lldP"
                     /locus_tag="QBH27_000578"
     CDS             55152..56807
                     /gene="lldP"
                     /locus_tag="QBH27_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDQ7012942.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFTSGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            56807..57583
                     /gene="lldR"
                     /locus_tag="QBH27_000579"
     CDS             56807..57583
                     /gene="lldR"
                     /locus_tag="QBH27_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDQ7012943.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFVGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            57580..58770
                     /gene="lldD"
                     /locus_tag="QBH27_000580"
     CDS             57580..58770
                     /gene="lldD"
                     /locus_tag="QBH27_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDQ7012944.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            58818..59291
                     /gene="trmL"
                     /locus_tag="QBH27_000581"
     CDS             58818..59291
                     /gene="trmL"
                     /locus_tag="QBH27_000581"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDQ7012945.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(59344..60165)
                     /gene="cysE"
                     /locus_tag="QBH27_000582"
     CDS             complement(59344..60165)
                     /gene="cysE"
                     /locus_tag="QBH27_000582"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDQ7012946.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(60245..61264)
                     /gene="gpsA"
                     /locus_tag="QBH27_000583"
     CDS             complement(60245..61264)
                     /gene="gpsA"
                     /locus_tag="QBH27_000583"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDQ7012947.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(61264..61731)
                     /gene="secB"
                     /locus_tag="QBH27_000584"
     CDS             complement(61264..61731)
                     /gene="secB"
                     /locus_tag="QBH27_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDQ7012948.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(61795..62046)
                     /gene="grxC"
                     /locus_tag="QBH27_000585"
     CDS             complement(61795..62046)
                     /gene="grxC"
                     /locus_tag="QBH27_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDQ7012949.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(62187..62618)
                     /locus_tag="QBH27_000586"
     CDS             complement(62187..62618)
                     /locus_tag="QBH27_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDQ7012950.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            62862..64406
                     /gene="gpmM"
                     /locus_tag="QBH27_000587"
                     /gene_synonym="pgmI"
     CDS             62862..64406
                     /gene="gpmM"
                     /locus_tag="QBH27_000587"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDQ7012951.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            64416..65699
                     /gene="envC"
                     /locus_tag="QBH27_000588"
     CDS             64416..65699
                     /gene="envC"
                     /locus_tag="QBH27_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDQ7012952.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            65703..66662
                     /locus_tag="QBH27_000589"
     CDS             65703..66662
                     /locus_tag="QBH27_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDQ7012953.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQLVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(66649..>67127)
                     /gene="waaH"
                     /locus_tag="QBH27_000590"
     CDS             complement(66649..>67127)
                     /gene="waaH"
                     /locus_tag="QBH27_000590"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDQ7012954.1"
                     /translation="LHHQDIVWTTEFMFNALRARYTEQSLYKYYLHNTSVSRLHRQGN
                     KNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCHAVRKEPDILTRQ
                     RMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT        16487 a        17460 c        17027 g        16153 t
ORIGIN      
        1 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
       61 tttttccagc atggtcagac ggttgatacg caattcttta tcatcaacca tgaccatcac
      121 tttatcgaag aaagcatcga ccggttcacg cagctcagcc agttcgacca gcgcatcctg
      181 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
      241 cagtttaatt tcttccggct ctttcagagt agaggcattc acgcgatcgc tcagcacttc
      301 atcagatttc gccaggatgt tagatacacg cttgttcgcc gccgccagtg ctgcagctgc
      361 ttccagggta cggaagtgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
      421 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
      481 ggcgcggaag cgaccgagca taaagtcgat aacatcatca accacgttgg cattagtcag
      541 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
      601 cttctcaacg ataatacgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
      661 gtcgcctttc ggatgctggc cgataccgaa gatacccgcc agggtatcca tcttgtcagc
      721 aatcgccagc gcacaggcta ccgggttaga cggcaggtca tcaccggcaa agcgcggctg
      781 atactgctca ttcagcgcga cagctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
      841 catccccata acgccctggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
      901 cttagacagc aggcccgcac gggtagcgtg gttaacgtca gcgccaatct gttcagcaat
      961 ccagccggcc agcgcctgaa tgcggtcagt tttgtcgcgc agcgtcccca actgttgctg
     1021 gaacaacacg gtttgcaggc gcggcaggtt atcttcaaga cgttttttac ggtcggtatt
     1081 gaagaagaac tcggcatccg ccaaacgtgg acgaacgact ttctcgttac cggagataat
     1141 ctgctgcgga tctttcgatt cgatgttggc aacgaagata aagttcggca gcagtttgcc
     1201 gtcgttcgca tacaccggga agtacttctg gtcacctttc atggtgtaaa ccagtgcttc
     1261 agacggcacc gcgaggaatt tctcttcgaa tttcgcggtc agaacgaccg gccactccac
     1321 cagcgaagcc acttcttcca gcaggctttc gcttaaatca gcgttaccgc caatcttacg
     1381 tgctgcttct tcagcatcgg ctttaatctt cgccttacgt tcttcgtaat cggcgatgac
     1441 tttcccgcgc tcacgcagaa tttctggata ctgatcggca ttgtcgatag tgaattccgg
     1501 ctcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaataccca gaatggtagc
     1561 cggaataact ttgtcgccca gcagcagggt cacggtgtga accggacgca caaagtgcac
     1621 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     1681 catattcggc agcagtgctt cggtgctttc gcccttcaca tgggcgcgat acagcagcca
     1741 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     1801 ccaaccttct gccgctttac tcggtttgcc ttcagcgtcg aacgcctgag caattgccgg
     1861 accacgtttt tcgatttcac gatccggttg cgcctccgcc aggttagcga ctttcagcgc
     1921 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     1981 ctccgcagta aagttcgcag caaaggactc agccaggctg cgtagtgctt ttggtggcag
     2041 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     2101 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     2161 tttggtcagg gtgcgaatgc gcagaatata gcgctgacgc tcggtgacgg agatggcttt
     2221 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     2281 cggcagcgga ttttccagcg ccagcagttg ctgcgcttct ttctcgtact gctcgaagca
     2341 ggtgaacagg aagtccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     2401 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     2461 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     2521 accggtaacc ggtttacatt ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     2581 ttccatgccg tttagccaca cttcccagcc cagcccccag gcacccagcg tcgggttttc
     2641 ccagttatct tccacgaaac ggatgtcgtg gatggtcggg tccatgccca gctctttcag
     2701 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     2761 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     2821 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     2881 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     2941 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     3001 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     3061 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     3121 aatggccatc cgcgtcaaaa gaacagacaa actgctcttt ccggaaggta taaccgcgca
     3181 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     3241 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     3301 caacctttac ccgttgtgct ttctgcacgg gtggcggtgt agaacaaccc actagcacaa
     3361 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     3421 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     3481 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aactgaatga tttatcggtc
     3541 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     3601 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgat
     3661 caatgctcat agtgttagcc ccatcacctg ggatatcgcc aatgttctga attctgcctc
     3721 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     3781 cgcccagtcg cgtcatttct tgcgtatcgg cttatgcctg tttttttata gcgcaatcgc
     3841 gctgctctac attgcgctgt ttacctccat caatgtggag ttagcgctga aaaacctgct
     3901 gcaaaagcca gtgttttacc atttatggtt tttcttcgcg attgcagtga tttatctggt
     3961 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggtggt
     4021 gattggtatc atcgctaacc caaacaccgt gccgcagaaa atcgacggtt ttgaatggct
     4081 gccaattaac ttatatatca atggcgatac tttttactac atcctttatg gcatgttggg
     4141 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcact
     4201 gtttgcgacg ggggttttta ttatctctcg tgggacatta tatgaattac agtggcgcgg
     4261 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     4321 gttattgact ctggttaaaa acacgctgta tacgcgtacc atttgcggac ttggcttaat
     4381 ctcccgccat tcgttaggta tatacgggtt ccatgcattg attatccatg cactgcgcac
     4441 ccgggggatt gagcttaaaa actggccaat actggatatt atttggatct tttgtgcgac
     4501 gctggcagtg agtttattac tttctatgct ggtacaacga atcgacagaa acagattagt
     4561 gagttaagta gaagcccggt cacattggac tgaccgggct tacgtgaatt attcgctgta
     4621 ttcttcctgg gtaacctgtg tttcttttgg tgggtttatt ccggcatcac gaatattctt
     4681 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agcccataaa agcctttttc
     4741 gctgagtaat aacgttgcat tccatagacc taccagcaac aacgcaacag agataataaa
     4801 gaccgtcagg caagtcatat aataaatggg ggtggtaggt atgccttcat acttgtcccg
     4861 aacggtcttt tgataagacg ccgcggaaaa caaacctaat accagtacgg caaaataata
     4921 ccctttttca tttaactgca tctctgcatt ccatagtcct aacagatagg taacaatacc
     4981 gccaataagg gctacccatg aaacaatact aaatgccgga gaataggttg atattttgtt
     5041 gtccatcaca gtattccttt cattcctgaa tgtgtaagaa ctttatgttg ctcatgccga
     5101 gcgaaacaaa cgcatttgac caaacaagca gacaaaaaag gcgatgggat agatcaccat
     5161 ttcataagca ttgataggtg cgtttaaaac accgataaac aagagaccga tagtgattaa
     5221 cgctaccaac tgacagacgt ggataaaaaa atcatccagt tgcccaagtt tttctgcgcg
     5281 aagatatgca tagttgccaa aagttgccgt cattaacacg ccaagaaaat agccttttcc
     5341 acttaacaat gggcatgaga tccatagccc aaccagataa accagcgccc cgacaacaaa
     5401 taatagtttt gccacagtct ttgaggtttt cataacgatc tccatatcta ccagcgatac
     5461 attacgagta accaacgaaa gacaaaactg aaaaatgcca ttaacaaatg attttcagaa
     5521 taaattcata ctaaatatta attaattatt tatatatata gatgtgaatt atcccccacc
     5581 cggtcaggca ggggataacg tttacgccat taatggcaga agttgctgat agaggcgacg
     5641 gaacgtttct cgtcgtggct gataagcggc ataacgctgc gcatctggta gatgcgactg
     5701 ttctaacggt agttgcggca acaactcaat gagcgatttc tctggattcg ccgcaatctg
     5761 cgccagcctt gctgcgccca gtgctggccc cacatcgcca cccgtgcggt aatccagttg
     5821 ctgaccgcta atgtccgcca gcatttgacg ccagtactca ctacgcgccc cgccaccaat
     5881 caacgtaaca ctttgcggtt taatgccgca ggcatgcacg acatccatgc catctgccag
     5941 cgcataaccc acgccttcca gcactgctcg cgccagttca ttggggccat gttgatgagt
     6001 caaaccaaag aaaacccctt tcgcctgggg attattgtgt ggtgtacgct cgccagaaag
     6061 ataaggcaga aaccaaactg gctcggcact ttcatcagcc tgtcgagctg cagtgattaa
     6121 agcagggaca ttgctcaggc cggttaattt cgcggcccaa tccagacacg aagccgcact
     6181 cagcattaca gacattaaat gccaacgttg cggtaacgca tggcaaaagc tatgtacggc
     6241 gctttctggc ttgcttaaga acccttcgct gacagcaaaa tagacccccg acgtccccag
     6301 cgataacatt gcctgattag catcaaccat tcccacacca accgcaccag ctgcattgtc
     6361 gccaccgcct gcgacaactg gcaccgccgc cataccccac gctttcgcaa cttcaggtag
     6421 caaagcacca gtaatttcgc tgccttcgta taatgcgggc atctggtcac gagataagtg
     6481 gcaagcctgc agcatgtcgt cactccagtc acgctttgcg acatccagcc acatggtgcc
     6541 agctgcgtca gacatatcgc tggcaaactc ccccgtcata cgcagacgca agtaatcttt
     6601 cggtaataat actttatcga tttgacggaa tatctccggc tcatgccgct gaacccatag
     6661 caatttaggc gcagtaaatc cgggcatcat caggttgccg gtaatcaccc gcgattgcgg
     6721 aactcgcgct tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc acaaaatggc
     6781 agggcgtaac acccgttgct gagcatccag taaggttgca ccatgcatct gtccggcaat
     6841 acctaatgct ttaacgtctt gcagagaatg ctgaacgccc agagctttca ttgcgcgatc
     6901 agttgcctgc caccactgtt ccgggtcttg ttccgaccag agtggatgcg ggcgcgaaac
     6961 ggtcagcttt tccgtttgcg cagcaaccac ctcaccctgc tcgttgagca aaataacttt
     7021 tacgcccgag gtgccaagat ctatcccgat atacatatcg atcgttcctt aaaaaatgcc
     7081 cggtatcgct accgataacc gggccaacgg actgcacagt taaccgttat ttgtcgaaca
     7141 gataatgatt taccagattt tccagttgct cctggcgacc actctgatgc accggagaca
     7201 aattatgttc ctgagcatat ttggctaaat ctgccagtga catttggcct ttcaggattt
     7261 gctggcctaa ttcactattc cagccggaat aacgctgcgc gatgcgttta tccagctcgc
     7321 catcttcaat catgcgcgct gcaattttca gcgccaacgc catcgtatcc atcgcgccga
     7381 tatgaccgta aaacagatca tatttatcag tactttgacg acgtactttg gcatcgaagt
     7441 tcagaccacc ggtggtgaaa ccgcctgctt tgagaatttc atacatcacc agcgcattct
     7501 cttccacact gttcgggaac tggtcggtgt cccagcccag ttgcgcatcg ccacggttgg
     7561 cgtcgacaga accgaacagg ccaagcgcaa tggcggtggc tatttcatga tggaaagagt
     7621 gacctgccag cgtcgcgtgg ttagcttcaa tgttcagttt aatctctttt tccagaccaa
     7681 actgtttcag gaagccatag accgtcgcag catcgtaatc atattgatgt ttggtcggtt
     7741 cttgcggttt cggttcgata agcaacgtgc cctggaaacc gattttatgt ttatgctcaa
     7801 ccaccatctg cataaagcgg cccagctgtt cacgctcctg gcgcaagtcg gtatttaaca
     7861 gcgtttcgta accttcacga ccaccccaca ggacatagtt ttcaccgccc aatttatggg
     7921 ttgcttccat cgctgtaaca acttgcgttg ccgcccaact gaagacttca ggatctgggt
     7981 tcgtcgccgc acccgcgcca tagcgagggt ttgtaaagca gttagcagtt ccccacagca
     8041 gcttcacgcc gctctcttct tgcttgcctg ccaggacatc aaccatttgc gcaaaattat
     8101 tgatgtactc ttttaacgac gcgccctcag gggaaacatc cacatcgtgg aagcaataaa
     8161 atggcacatg taacttgtgg aaaaactcaa atgcgacatc tgctttacgc ttcgccaacg
     8221 ccagtgcctc accaggctgc tgccacggac gattaaacgc ccccacacca aacatatccg
     8281 ccccgttcca gcagaaggtg tgccagtagc aggcggcaaa acgcaagtgc tcttccatac
     8341 gcttgcccaa caccagttcg tcgggattgt agtgacggaa tgctaacggg tttgaggatt
     8401 ttgagccttc ataacgaacg cgatcgagct ggtcaaaata ggcttgcata ttgaactcca
     8461 taatcaggta atgccgcggg tgatggatga tgtcgtaata ttgggcactc cctttcagtt
     8521 gctcaattat gttatttcac actgctattg agataattca caagtgtgcg ctcgctcgca
     8581 aaataaaatg ggatgatgaa actgggtaat tcctcgaaga gaaaaatgca ataagtacaa
     8641 ttgcgcaaca aaagtaagat ctcggtcata aatcaagaaa taaaccaaaa atcgtaatcg
     8701 aaagataaaa atctgtaatt gttttcccct gtttagttgc taaaaattgg ttacgtttat
     8761 cgcggtgatt gttacttatt aaagctgtcc tctaactaca gaaggcccta caccatgaaa
     8821 ataaagaaca ttctactcac cctttgcacc tcacttctgc ttaccaacgt tgcggcacac
     8881 gccaaagaag tcaaaatagg tatggcgatt gatgatctcc gtcttgaacg ctggcaaaaa
     8941 gatcgagata tttttgtgaa aaaagcagaa tctctcggcg cgaaagtatt tgtacagtct
     9001 gcaaatggca atgaagaaac acaaatgtcg cagattgaaa acatgattaa ccggggtgtc
     9061 gatgttcttg tcattattcc ttataacggt caggtattaa gtaacgttgt aaaagaagcc
     9121 aaacaagaag gtattaaagt attagcttac gaccgtatga ttaacgatgc ggatatcgat
     9181 ttttatattt ctttcgataa cgaaaaagtc ggtgaactgc aggcaaaagc cctggtcgat
     9241 attgttccgc aaggtaatta cttcctgatg ggcggctcgc cggtagataa caacgccaag
     9301 ctgttccgcg ccgggcaaat gaaagtatta aaaccttatg ttgattccgg aaaaattaaa
     9361 gtcgttggtg accaatgggt tgatggctgg ttaccggaaa acgcattgaa aattatggaa
     9421 aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc
     9481 gcaggagggg caattcaggc attaagcgcg caaggtttat cagggaaagt agcaatttcc
     9541 ggccaggatg cggatcttgc aggtattaaa cgtattgctg ccggtacgca aactatgacg
     9601 gtgtataaac ctattacatt gttggcaaat actgccgcag aaattgccgt tgagttgggc
     9661 aatggtcagg agccaaaagc ggataccaca ctgaataatg gcctgaaaga tgttccctcc
     9721 cgcctgctga caccgatcga tgtgaataaa aacaacatca aagatacggt agttaaagac
     9781 ggattccaca aagagagcga gctgtaagcg ttacgccccg gcgcggagcg ggggcgtgat
     9841 ttctctccat gccgcgttaa ttaattggct taggtggagt cgttatgcct tatctacttg
     9901 aaatgaaaaa tattaccaaa accttcggca gtgtgaaggc gattgataac gtcagcttgc
     9961 ggttgaatgc tggcgaaatc gtctcactct gtggggaaaa tgggtcaggt aaatcaacgc
    10021 tgatgaaagt gctgtgtggt atttatcccc atggttccta cgaaggcgaa attatttttg
    10081 cgggagaaga gattcaggcg agtcacatcc gcgataccga acgcaaaggt atcgccatta
    10141 ttcaccagga attggcgctg gtgaaagaac tgaccgtgct ggaaaatatc ttcctgggta
    10201 acgaaataac ccataatggc attatggatt atgacctgat gacgctacgc tgtcagaagc
    10261 tgctcgcaca ggtcagttta tccatttcac ctgatacccg cgttggcgat ttaggacttg
    10321 ggcaacaaca actggttgaa attgccaagg cacttaataa acaggtgcgt ttgttaattc
    10381 tcgatgagcc gacagcctca ttaactgagc aggaaacgtc ggttttactg gatattattc
    10441 gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc aacgaagtca
    10501 aagcgatttc cgatacgatt tgcgttattc gcgacggtca acacattggt acgcgtgatg
    10561 ctgctggaat gagtgaagac gatattatca ccatgatggt cgggcgagag ttaaccgcgc
    10621 tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa catctgacgg
    10681 catggcatcc ggttaatcgt catattaaac gagttaatga tgtctcgttt tccctgaaac
    10741 gtggcgaaat actgggtatc gccggactcg ttggtgccgg acgtaccgag actattcagt
    10801 gcctgtttgg tgtgtggccc ggacaatggg aagggaaaat ttatattgat ggcaaacagg
    10861 tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggta cccgaagaca
    10921 gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc ctcgccgcac
    10981 tcaaaaaatt taccggtagt attagccagc ttgatgacgc ggcagagcaa aaatgtattc
    11041 tggaatcaat ccagcaactc aaagttaaaa cgtcgtcccc cgaccttgct attggacgtt
    11101 tgagcggcgg caatcaacaa aaagcgatcc tcgctcgctg tctgttactt aacccgcgca
    11161 ttctcattct tgatgaaccc accaggggta tcgatattgg cgcgaaatac gagatctaca
    11221 aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct tccgaattac
    11281 ctgaagtgct cggccttagc gatcgtgtac tggtgatgca tgaagggaaa ctaaaagcca
    11341 acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg aggagcgaac
    11401 atcatgtcga aaagcaatcc gtctgaagtg aaattagccg taccgacatc cggtggcttt
    11461 tccgggctga aatcactgaa tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc
    11521 atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa cgtctccaac
    11581 ctgttacgcc aaaccgcaat taccggcatc ctcgcggtag gaatggtgtt cgtcataatt
    11641 tctgctgaaa tcgacctttc cgtcggctca atgatggggc tgttaggtgg cgtcgcggcg
    11701 atttgtgacg tctggctagg ctggcctttg ccacttacca tcattgtgac gttggttctg
    11761 ggactgcttc tcggagcctg gaacggatgg tgggtcgcgt accgcaaagt cccttcattt
    11821 attgtcaccc tcgcgggcat gttggcattt cgtggcatac tcattggcat caccaacggc
    11881 acgactgttt cccccaccag cgccgcgatg tcacaaattg ggcaaagcta tctacctgcc
    11941 agcaccggct tcatcattgg cgcgcttggc ttaatggctt ttgttggctg gcaatggcgc
    12001 ggaagaatgc gccgtcaggc tttgggtttg cagtctccgg cctctaccgc agtagtcggt
    12061 cgccaggctt taaccgctat catcgtatta ggcgcaatct ggctgttgaa tgattaccgt
    12121 ggtgttccca ctcctgttct gctgctaacg ttactgttat tgggcggcat gtttatggca
    12181 acgcggacgg catttggacg gcgcatttat gccatcggcg gcaatctgga agcggcacgt
    12241 ctctccggga ttaacgttga acgcaccaaa cttgccgtgt tcgctattaa cggattaatg
    12301 gtagccatcg ccggattaat cctcagttct cgactcggcg ctggttcacc ttctgcggga
    12361 aatatcgccg aactggacgc aattgcagca tgtgtgattg gcggcaccag tctggcaggc
    12421 ggcgtcggga gcgtggccgg agcggtaatg ggggcattca tcatggcttc gctggataac
    12481 ggcatgagta tgatggatgt accgaccttc tggcagtata tcgttaaagg tgcgattctg
    12541 ttgctggcag tatggatgga ctccgcaacc aaacgccgtt cttgattttg ataaaaattt
    12601 tctcaaagcc ggtaacgtat taccggtttt gagtttttgc atgattcagc aggaaaagaa
    12661 ccatgtttac taaacgtcac cgcatcacat tactgttcaa tgccaataaa gcctatgacc
    12721 ggcaggtagt agaaggcgta ggggaatatt tacaggcgtc acaatcggaa tgggatattt
    12781 tcattgaaga agatttccgc gcccgcattg ataaaatcaa ggactggtta ggagatggcg
    12841 tcattgccga cttcgacgac aaacaaatcg agcaagcgct ggctgatgtc gacgtcccca
    12901 ttgttggggt tggtggctcg tatcaccttg ccgaaagtta cccacccgtt cattacattg
    12961 ccaccgataa ctatgcgctg gttgaaagcg catttttgca tttaaaagag aaaggcgtta
    13021 accgctttgc tttttatggt cttccggaat caagcggtaa acgttgggcc actgaacgcg
    13081 aatatgcatt tcgtcagctt gtcgctgaag aaaagtatcg cggagtggtt tatcaggggt
    13141 tagaaaccgc gccagagaac tggcaacacg cgcaaaatcg gctggcagac tggctacaaa
    13201 cgctaccacc gcaaaccggg attattgccg ttactgacgc ccgggcgcgg catattctgc
    13261 aagtatgtga acatctacat attcccgtac cggaaaaatt atgcgtgatt ggcatcgata
    13321 acgaagaact gacccgctat ctgtcgcgtg tcgccctttc ttcggtcgct cagggcgcgc
    13381 ggcaaatggg ctatcaggcg gcaaaactgt tgcatcgatt attagataaa gaagaaatgc
    13441 cgctacagcg gattttggtc ccaccagttc gcgtcattga acggcgctca acagattacc
    13501 gctcgctgac cgatcccgcc gttattcagg ctatgcatta cattcgtaat cacgcctgta
    13561 aagggattaa agtggatcag gtactggatg cggtgggcat atcacgctcc aatcttgaga
    13621 agcgttttaa agaagaggtg ggtgaaacca tccatgccat gattcatgcc gaaaagctgg
    13681 agaaagcgcg cagtctgctg atttcaacca ccttgtcgat caatgagata tcgcaaatgt
    13741 gcggttatcc atcgctgcaa tatttctact ctgtttttaa aaaagcatat gacacgacgc
    13801 caaaagagta tcgcgatgta aatagcgagg tcatgttgta gcgatgatga gaattgtcgg
    13861 cgtcgcttca ggtaactcag cacaaccacc tgatgggagc aaaagcctta tccgactgac
    13921 aagtcggata aggctcgagg aaatgcaatt acatatgcgc cgcgattaac cgttggttat
    13981 cctggtacat tgcaaacagg tagttgttat aactcttccc tttggtcgag tagcccttca
    14041 gcttgtgaat catcgctgtg gcagtcactt cctgatccgc tttacgcagc tgtgcacgcg
    14101 atttacggaa cgaagagtaa gccgggtgcg tattgaggtt tgtaacatag gcgctcaccg
    14161 attctttaac agaactaaac tgtgagtacc ctttcacttt acccggcgca ttggtacaac
    14221 gtcctttcat gcatttcatg ccgaaaagat tgttgttgtt gcgcgccagc tttgacgttc
    14281 cccaaccgct ttctgctgca gccatcgtcg ccaccatact ggtagggata atgtctacgc
    14341 gttcaagcaa ggtattccac gggatttttc gcgtattacc agaccacttc accttgtagc
    14401 gtttggcgat gtctttcaga cgcgcacgct cagcaggtga ccattggccc tgatactgtt
    14461 ttgaaatcag ccagttacgt tccgcagtaa tggccgcatt ttggctggta atgtaaggca
    14521 ttacagtccg gagaaacgcc tttttccttg gtgttccgga agggtatttt cgcaaatcag
    14581 gaagtgaact actctttgca ctattgcgag aatactcttg tttactgctt acctgtttat
    14641 tactagcttt agttaagtgg gacttttgac tcgctgttgt tgtgtgcgtc ttcgctaaca
    14701 cctcactcga aaacaccaga gtgagtaaca taagaatcat cgccccatat cgtcgtatgg
    14761 gagtcaaaat catcaggtct cctggtcgga tttaatcatt ccaacacctt atatttttta
    14821 caaatttgag agttgaatct caaatcatat caaaaatagc tgtcaagagc accccaagga
    14881 atagtccaaa tctgaaacta tgtcacgagt taacgattca gattggcgct aaatcgcaga
    14941 aaatgtgggg tttatcgcaa aattcagccg ttttttgcgc gcgatcgctc acccttgctt
    15001 ctcatcctgt ggagttaccg ctcagggatg agttttgtct ggcttatcgc tggcaaactg
    15061 tctgaaatcg cagcaataag gactcatccg ccatgaaact cgccgcctgt tttctgacac
    15121 tccttcctgg cttcgccgtt gccgccagct ggacttctcc ggggtttccc gcctttagcg
    15181 aacaggggac gggaacattt gtcagccacg cacagttgcc caaaggtacg cgtccactaa
    15241 cgctaaattt tgaccaacag tgctggcagc ctgcggatgc gataaaactc aatcagatgc
    15301 tttccctgca accttgtagc aacacgccgc ctcaatggcg attgttcagg gacggcgaat
    15361 atacgctgca actagacacc cgatccggta caccaacatt gatgatttcc atccagaacg
    15421 ccgtcgaacc ggtagcaagc ctggtccgtg aatgcccgaa atgggatgga ttaccgctca
    15481 cactggatgt cagcgccact tttgcggagg gagccgcagt ccgggattat tacagccagc
    15541 aaattgcgat agtgaagaac ggtcaaataa cgttacaacc cgctgccacc agcaacggtt
    15601 tactcctgct ggaacgggca gaaactgaca catccgcccc tttcgactgg cataacgcca
    15661 cggtttactt tgtgctgaca gatcgtttcg aaaacggcga tcccagtaat gaccagagtt
    15721 acggacgtca taaagacggt atggcggaaa ttggcacttt tcacggcggc gatttacgcg
    15781 gcctgaccaa caaactggat tacctccagc aactgggcgt caatgcttta tggataagtg
    15841 ccccatttga gcaaattcac ggctgggtcg gcggcggtac aaaaggtgat ttcccgcatt
    15901 atgcctatca tggttattac acacaggact ggacgaatct tgatgccaat atgggcagtg
    15961 aagccgatct acggacgctg gttgatagcg cgcatcagcg cggtattcgt attctctttg
    16021 atgtcgtgat gaaccacact ggctatgcca cgctggcgga tatgcaggag tatcagtttg
    16081 gcgcgttata tctttctggt gacgaagtga aaaaaacgct gggtgaacgc tggaacgact
    16141 ggaaacctgc cgccgggcaa acctggcata gctttaacga ttacattaat ttcagcgaca
    16201 aaacaggctg ggataaatgg tggggaaaaa actggatcag aacggatatc ggcgattacg
    16261 acaatcctgg attcgacgat ctcactatgt cgctggcctt tttgccggat atcaaaaccg
    16321 aatcaactac cgcttctggt ctgccagtgt tctataaaaa caaaacggat acccacgcta
    16381 aaatcatcga cggctttacc cctcgcgatt acttaaccca ctggttaagt cagtgggtcc
    16441 gcgactatgg gattgatggt tttcgggtcg ataccgccaa acatgttgag ttgcccgcct
    16501 ggcagcaact gaaaaccgaa gccagcgctg cacttcacga atggaaaaaa gctaaccccg
    16561 acaaagcatt agatgacaaa cctttctgga tgaccggtga agcctggggc cacggcgtga
    16621 tgcaaagtga ctactatcgc cacggcttcg atgcgatgat caatttcgat tatcaggagc
    16681 aggcggcgaa agctgtcgat tgtctggcgc agatggatac gacctggcag caaatggcgg
    16741 agaaattgca ggatttcaac gtgttgagct acctctcgtc gcatgatacc cgcctgttcc
    16801 gtgaaggggg cgacaaagca gcagagttat tattattagc accaggcgcg gtacaaattt
    16861 tttatggcga tgaatcctcg cgtccgttcg gtcctaccgg ttctgatccg ctgcaaggta
    16921 cgcgttcgga tatgaactgg caggatgtta gcggtaaatc tgccgccagc gtcgcgcact
    16981 ggcagaaaat cagccagttc cgcgcccgcc atcccgcaat tggcgcgggc aaacaaacga
    17041 cactttcgct gaagcagggc tacggctttg ttcgtgagca tggcgacgat aaagtgctgg
    17101 tcatctgggc ggggcaacag tgacttttcc ggcttcccgt tcgtgagtac ctcgggaagc
    17161 cgccaaccag gataaaatgt cagccctaat cagcattgca ggataaagcc ccgctcactc
    17221 ttcaacagac cgatttgcac cccagcaaat gtagcgttat tgttaccttc cttgctacag
    17281 agttcgacag atatcccgct atgacattct ccctttttgg tgacaaattt acccgccact
    17341 ccggcattac gctgctgatg gaagatctga acgacggttt acgcacgcct ggcgcgatta
    17401 tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca ggactacttc cagacgctac
    17461 tgaccgacat gctggaaagt ggcaaagcga ctgatgcact gtgcaattac gacggtccac
    17521 aggggaaaac ggagctactc acactgcttg ccggaatgct gcgcgagaag ttgggctggg
    17581 atatcgaacc acagaatatt gcactaacaa acggcagcca gagcgcgttt ttctacttat
    17641 ttaatctgtt tgccggacgc cgtgccgatg gtcgggtcaa aaaagtgctg ttcccgctgg
    17701 caccggaata cattggctac gctgacgccg gactggaaga agatctgttt gtctctgcgc
    17761 gtccgaatat tgaactgctg ccggaaggcc agtttaaata ccacgtcgat tttgagcatc
    17821 tacatattgg cgaagaaacc gggatgattt gcgtctcccg gccgacgaat ccaacaggca
    17881 atgtgattac tgacgaagag ttgctgaagc ttgacgcgct ggcgaatcaa cacggcattc
    17941 cgctggtgat tgataacgct tatggcgtcc cgttcccggg tatcatcttc agcgaagcgc
    18001 gcccgctatg gaatccgaat atcgtgctgt gcatgagtct ttccaagctg gggctacctg
    18061 gctcccgctg cggcattatc atcgccaatg aaaaaatcat caccgccatc accaatatga
    18121 acggcattat cagcctggca cctggcggta ttggtccggc gatgatgtgt gaaatgatta
    18181 agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa accgttttac taccagcgtg
    18241 ttcaggaaac tatcgccatc attcgccgct atttaccgga agatcgctgc ctgattcata
    18301 aaccggaagg agccattttc ctctggctat ggtttaagga tttgcccatt acgaccgagc
    18361 agctctacca gcgcctgaaa gcacgcggcg tgctgatggt gccggggcac aacttcttcc
    18421 cgggactgga taaaccgtgg ccgcatacgc atcaatgtat gcgcatgaac tacgtaccag
    18481 agccggagaa aattgaggcg ggtgtgaaga ttctggcgga agagatagaa agagcctggg
    18541 ctgaaagcca ttaatacgct attccggcgt tgagaaacgc cggaaaaacg tttcaaacag
    18601 gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg
    18661 tcgggcaggc ttcaacacag gccggccccg tcgcccgatg ccagcacaga tcgcacttaa
    18721 tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca
    18781 tacagctttt acagccaatg caacgtgatt gttcaacgaa aatatgccca tgttcgcggc
    18841 ttatcgcgtc aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta
    18901 ctgccgtggt ccagctgtgg tctttaatga cacgaatacg ggaaataaac tcgtctggtg
    18961 acaacgcagc gcaatcctga ttctcctgat gcgacactgc gcaagccact tcacaggtac
    19021 ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc actcctttgc
    19081 actaaaacag cagagtgcca gagagcgcgt ccctactgct ttgatccaac gaaggaaatg
    19141 ttaaagtttt ggcacttgtc caaagggtta tgtaatcgcg ccctggtcgt ccctgacagt
    19201 aaatcccagc tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt
    19261 ctctcccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt acggcacccg
    19321 cccatgaata tcaaacaccg gaacagcaat acaggagacg ccgagttcgt tttcttctct
    19381 gtccatcgcc gctccacttt cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc
    19441 ggtaatggta ttgcgggtta acggctggat ctcatgctga tggctttccc agtatgactt
    19501 cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac agtagagcgg
    19561 catatgctgg cctatatagg cacgggttcg cagcatcccg gttgtgggtt ccagcttata
    19621 aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atggtttcac cagtggcgat
    19681 gttcagtgcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag atagcgcctt
    19741 ctgcccgacg gcaataaatt tggtggtcag gcgataactc cctgcggcgg gcgcggtggt
    19801 cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct tatttaaacc
    19861 agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca aaatctcaat
    19921 cagcatcaac ccacgaaaca gactctggct tccggctgga cgctcttttt cctgcgccat
    19981 ctcgttctct ttttttccca tcacttcttt ccccattttg tcgcgtcctg atggtagcgc
    20041 aaagtgtgcc gtagttcacg atctcgacag ataatttata accacttgat ttttaagttt
    20101 tttgaaattc atcaatcaga ttgctttgtt aaaaagtgat cgatatattt gaaatcaagt
    20161 ttcgtatatt gaaattttaa gccaaaaaag cgatcaaaaa aacaaggaag cctgggatga
    20221 aagtgacatt tgagcagtta aaagcagcct ttaatcgggt cttaatttca cgcggcgttg
    20281 acagcgaaac ggctgacgcc tgtgcagaga tgttcgcccg caccaccgaa tccggcgttt
    20341 attctcacgg cgttaatcgt ttccctcgtt tcattcaaca actggaaaac ggcgatatca
    20401 ttcctgatgc ccaacccaaa cgtataacca gcctcggcgc aattgaacag tgggacgccc
    20461 agcgttcgat cggtaacctg acagcgaaaa agatgatgga tcgcgccatt gaactggctg
    20521 ccgatcacgg tattggtctg gtggcactac gtaatgccaa ccactggatg tgcggcggca
    20581 gctacggctg gcaggcggcg gaaaaaggct acattggcat ttgctggacc aactccatcg
    20641 ccgtaatgcc accgtggggc gcaaaagagt gtcgcatcgg caccaacccg ctgatcgtcg
    20701 ccattccttc cacgccgatc accatggtcg atatgtcgat gtcgatgttc tcttacggca
    20761 tgttagaagt taatcgcctg gctggtcgtc agctcccggt agatggtggc tttgatgatg
    20821 agggcaattt gaccaaagaa cctggcgtta tcgagaaaaa tcgccgcatt ttgccgatgg
    20881 gctactggaa aggttcgggc atgtcgattg tgctggatat gatcgctacc ctcctttctg
    20941 acggcgcatc ggttgccgaa gtcaccgagg acaacagcga cgaatacggc atttcacaaa
    21001 tttttattgc cattgaagtg gacaagctta tcgacggtcc cacccgcgat gccaaactgc
    21061 aacgcattat ggattacgtt accagtgccg agcgtgctga cgaaaatcag gccattcgct
    21121 tacccggcca cgaatttact accctgctgg cagaaaaccg ccgtaacggt attaccgttg
    21181 atgacagcgt gtgggcaaaa atccaggcgt tataaggaga taagccatga tttttggaca
    21241 tatcgcgcag cctaatccgt gccgtttgcc cgccgccatt gaaaaggcgc tcgattttct
    21301 gcgcaccacc gacttcaacg ccctggatcc gggcgttgtc gaaatcgacg gcaagaatat
    21361 ttatgcgcaa attatcgatt taaccacacg cgaagcggtg gaaaaccgtc cggaagtgca
    21421 tcgtcggtat atcgatattc agtttctggc gtggggtgaa gagaaaattg gcattgccat
    21481 tgatacggga aacaataaag tcagcgaatc actattagag cagcgcgata ttatttttta
    21541 tcacgatagt gaacatgaat catttataga aatgataccg ggtagttacg ctattttctt
    21601 cccgcaggat gtacatcgcc cggcatgtaa taaaaatgcc actacggcaa tacgcaagat
    21661 cgtggttaaa gtggccctcg ctgctttata aaaaatgatt actggagcac ataatgaaaa
    21721 cgaataatgc cggttatatt atcggcgcgt atccctgtgc accttctttt caccaaagaa
    21781 gtgaagagga agagaaggat ttctggcgac aattatctga gactccagac attttgggtc
    21841 tggaacaacc ttgcctggaa aattttcacc ctcttggcga tcaatggtta ctccgtcata
    21901 cgccagagag ctggaaattt gtcgtcaccg cagtgatgga aaccatgcgt cgccgcagcg
    21961 aaaacagcgg gtttggtctc gcctccagtg atgaagaaca gcgccaggcc tgcgtggcgt
    22021 actatcgcca cctgttcaat aaaatcaacg cattgcaggc taataaagtg ctcgcactgg
    22081 cgctccaggc tgcaccgtta gcaacgaatc ctaatgttat gcaagcgacg gacgcttttg
    22141 cccgctcatt gaaagaaatc gccagttggg actggccgtg caagctggtg ctggaacact
    22201 gcgacgccat gacatcttct tccccccgaa aaggcttttt gcctttagaa aatgtgctgg
    22261 aagttattac cgattatgac atcagcatct gtatcaactg ggcgcgctct gccattgaag
    22321 ggcgcaatac aacgctgccg ctgacccaca cccaaatggc gaaacaagcc ggaaaactcg
    22381 gcgcgttgat gttctctggt acgacgctaa acggggcata cggcgaatgg caggatttac
    22441 acgcaccgtt tgcaccattc tgcgcagaaa gcctgatgac cacagatcat gttcgcgagt
    22501 tatttaatgt agcagaatcg tcaacattgc attttgctgg catcaagcta ctggaaataa
    22561 atgcgacggc tgatgtgcac catcgcatcg aaatattacg taacggcatt cactctctga
    22621 atgaatctcg ttaaatagat ccgcttatat atcactttga atatctaatt agccgttttc
    22681 tgataattat tacgaatatt aacaacatta gcgcataaaa aatattgcat aaccagaagg
    22741 actcgttatg aaacggatac tcgaaggcat tcttgcgatc atcatcgcaa tactgtcttg
    22801 tattattttt attaatatca ttcttcgata tggattccac acaagtatat tatcgattga
    22861 tgaattatcc cgacttttat ttgtctggtt aacctttatt ggcgcaatcg ttgcttatat
    22921 ggacaacagc catgtgcagg ttactttttt agtcgaaaaa ctctcaccgg ctaatcaaca
    22981 acgtgtttcg ctgctaaccc atacgctcat tttattgctg tgtctggggc tggcctgggg
    23041 cgctatagaa aaaacagcgc aagactggag caacttatcg cccattcttg gcgtccccgt
    23101 tggcttaatg tacgccgccg ctatccccac cagtctgatt atcgcattgc tggaattgcg
    23161 tcacttatac cggcagttca caaatacccc cagccgaaac caacaaggag cctgacgatg
    23221 acaatcctta tatttattgt cagtttgtta ggggctatcg caattggtgt tcccatcgcc
    23281 tgggcattat tgttgtgcgg catctcattg atgttctgga tggatatctt tgatgtgcaa
    23341 attctggcac aaacgttggt aaatggcgca gacagcttct ccctgttagc catccccttc
    23401 tttgtactgg cgggtgagat catgaacgcg ggcggcctgt cgcaacgtat cgtcgacttg
    23461 ccaatgaaac tggtaggcca caggccaggc ggtctgggtt acgtgggcgt gctggcagcg
    23521 atgatcatgg caagcctgtc cggttctgcg gttgctgata ccgcagctgt cgcggcgctg
    23581 ctggtgccga tgatgcgcca ggccaactat ccggtaaacc gcgccgccgg gcttatcggt
    23641 tccggcggga ttattgcggc cattatccca ccttccatac cactcattat cttcggcgtc
    23701 tccagcggac tctccatcag taagctgttt atggcgggca tcgcaccggg catcatgatg
    23761 ggcgtcacgc tgatggttac ctggtggtgg caggcaaaac gactgaattt accttgtcag
    23821 ccgaaagcct cactgcgaga agtctggcaa tccttagttt ccggtatttg ggcgctcttt
    23881 ttaccgataa ttattattgg cggtttccgt tcggggttat ttacaccgac cgaagcaggc
    23941 gcggttgccg ccttctacgc tctgttcgtc tcggtggtgg tctatcgcga gatgaccttc
    24001 tccacgcttt atcatgtgct gattaacgcg gcaaaaacca catccgtggt gatgttcctg
    24061 gtggcctctg ctgcggtatc tgcatggctt attacgatcg ccgaattacc catgatggta
    24121 tcggagctat tacaaccgtt ggttgattcg cctcgcttat tattcatcgt cattatgctc
    24181 gctattatgg tgatcagtac ggtcatggac ttaacgccta ccgtattaat tcttacacca
    24241 gtattaatgc cattagtgaa agaagcggga atagatccgg tctatttcgg catcatgttt
    24301 attatcaact gttcaatatc actgattacg cctccagtcg gtaatgttct taacgtggta
    24361 tgcggcgtgg cgaaacttaa atttgacgat gcggtaaaag gcgttgcgcc ttacgtcatg
    24421 gttttattca tgttgttagc attatttatt tttataccgg agctcattac cgctcctctt
    24481 aaatggatga gttaaaagga aatcataatg aaattacgtt ctgtaaccta cgctttatta
    24541 attgccggag tggcgacgtt cagcacatct tctctggcgg cacaatcatt acgtttcggt
    24601 tatgaaacct cacaaaccga ttcacaacat attgcggcga aaaaatttaa tgaattactg
    24661 caggaaaaaa ccaaaggcga actgaaatta aaactgttcc cggatagcac ccttggtaat
    24721 gcgcaggcga tgatcagcgg cgtgcgtggc ggcactattg atatggagat gtctggctct
    24781 aacaacttta cgggactctc gcccgtcatc aacctgctcg atgtcccgtt cctgttccgc
    24841 gataccgctc acgcgcataa aacgctcgac ggcaaagtcg gtgatgacct gaaagtctcc
    24901 cttgaaggta aagggctgaa agtgctggct tactgggaaa acggctggcg cgatgtcacc
    24961 aactcacgcc agccagttaa aacacctgcc gacctgaaag gactaaaaat ccgcaccaac
    25021 aacagcccga tgaatatcgc cgcatttaaa gtttttggcg ctaacccgat cccgatgccg
    25081 tttgccgaag tctataccgg gctggaaacc cgcactatcg acgctcagga acacccgatc
    25141 aacgtcgtct ggtcagcaaa atttttcgaa gtgcagaagt acctttctct gacccatcac
    25201 gcctattccc cacttctggt ggtgatcaac aaagcgaagt ttgatggctt aaccccggag
    25261 ttccagcagg cactgatctc atctgcgcag gaagcgggta gctatcagcg caaactggtc
    25321 gccgaagatc agcaaaaaat catcgacggt atgaaagaag cgggcgtgga agtcatcacc
    25381 gatctcgacc gcaaagcctt tagcgatgcg ctgggtaccc aggtccgtga catgtttgtt
    25441 aaagatgttc cccagggtgc tgatctgctg aaagccgtgg atgaggtgca ataaaccatg
    25501 acgcaatact ggctggggtt agattgtggc ggtagctggc tgaaagccgg gctgtatgac
    25561 cgagaaggcc gggaggcggg cgtgcagcgc ctgccgctgt gcgcattaag tccacagcca
    25621 ggctgggcag agcgcgacat ggcagaactg tggcaatgtt gcactgctgt cattcgctcc
    25681 ctgcttaccc attctggcgt cagcggggaa caaattgtcg gtatcggcat ctccgcgcag
    25741 ggaaagggct tatttctact tgataaaaac gacaaaccgc tcggtaacgc cattttgtcc
    25801 tcggatcgcc gggcgatgga aatcgttcgt cgctggcagg aagatggcat cccggaaaaa
    25861 ctctatccgc tgacccgaca aaccttatgg accgggcatc cggtgtcgct gttacgctgg
    25921 ctgaaagagc acgaaccaga acgctacgcg caaattggct gcgtgatgat gacgcacgac
    25981 tacctgcgct ggtgtttaac cggcgtcaaa ggttgcgaag agagcaatat ttctgagtcc
    26041 aatctctaca acatgagtcg cggggaatat gacccgtgcc taaccgactg gctggggatc
    26101 gctgaaatca atcacgccct gccgcctgtt gtcggatctg ccgaaatctg cggggaaatc
    26161 accgctcaga cagccgtact gactggtctg aaagcgggta cccccgttgt cggcggcctg
    26221 tttgatgtgg tttccaccgc actctgcgcc gggctcgaag acgaattcac cctcaatgcg
    26281 gtgatgggga cctgggcggt gaccagcggc ataacccacg gtttacgtga cggtgaagcg
    26341 catccgtatg tctatggtcg ctacgttaac gatggtcaat ttatcgttca cgaagccagc
    26401 cccacctctt ccggcaatct cgaatggttt accgcacagt ggggagaaat ctcatttgct
    26461 gagattaatc aggccgtagc cagtttgccg aaagccggtg gcgctctctt tttcctgccg
    26521 ttcctgtacg gcagcaacgc cggactcgaa atgaccagcg gtttctacgg gatgcaggcc
    26581 attcacaccc gtgcacacct cttacaggct atctatgaag gggtggtgtt cagccatatg
    26641 acccatctca accggatgcg cgaacgtttt actgatgtgc acaccctgcg cgtcactggc
    26701 ggcccggcgc actccgatgt ctggatgcaa atgctggctg acgtcagcgg tctgcgtatc
    26761 gagctgccgc aggtggaaga aaccggctgc tttggtgcgg cacttgccgc ccgcgtcggt
    26821 accagggttt atcgcgattt cagcgaagcc caacgtgact tacagcaccc ggtacgcacc
    26881 ctgctgccgg atatggccgc ccatcagctt taccaacaaa aataccaacg ctatcaacat
    26941 ctcattgccg cacttcaggg ttatcacgcc cgcatcaagg agcacacatt atgagtcgac
    27001 cacttctgca actggccctc gaccactcat cacttgaagc cgcgcagcgc gacgtgacgc
    27061 tgttaaaaga cagcgtcgat attgtcgaag caggcaccat tctctgttta aacgaagggc
    27121 ttggcgcagt gaaagccttg cgcgaacagt gcccggacaa aatcatcgtt gccgactgga
    27181 aggtcgccga cgcgggtgaa acgctcgcgc aacaggcatt tggcgcaggc gctaactgga
    27241 tgaccatcat ctgcgccgcg ccgctcgcca cggtggaaaa aggccacgcg atggcacaga
    27301 actgcggcgg tgaaattcag atcgagctat tcggcaactg gacgctggac gatgcccgtg
    27361 actggcatcg tattggtgta cgacaggcga tttatcatcg tggtcgtgac gcgcaggcca
    27421 gcgggcaaca gtggggcgag gccgatctgg cacgcatgaa ggcgctttcc gatatcggcc
    27481 ttgagctttc cattaccggc gggattaccc ctgctgacct gccgctgttt aaagagatcc
    27541 gcgtgaaagc gtttattgcc ggacggtcgc tggcaggcgc agcaaatccg gcgcaagtgg
    27601 ccgctgattt tcatgcgcaa atcgactcca tctggggagg caagcgtgcg taatcatccg
    27661 ttaggtattt atgaaaaagc gctggcgaaa gatctctcct ggccggagcg gctggtgctg
    27721 gcaaaaagct gtggttttga ttttgtcgaa atgtcggtgg atgaaaccga cgaacggttg
    27781 tcacgtcttg actggagcgc cgcacaacgt gcttcgctgg tcactgcgat gctcgaaacg
    27841 ggtgttgcta ttccgtcaat gtgcctgtcc gcacatcgtc gttttccttt tggtagccgg
    27901 gacgacgccg tgcgccagcg ggcgcgggag attatgagta aagcgattcg cctggcacgc
    27961 gatctcggca ttcgtactat tcaactggca ggttacgacg tctattacga agatcatgac
    28021 gaaggcaccc agcaacggtt tgctgaaggg ctggcgtggg cggttgaaca ggcagcggca
    28081 tcacaagtca tgctggcggt ggagattatg gacaccgcgt ttatgaactc tatcagcaaa
    28141 tggaaaaagt gggacgaaat gcttgcgtca ccgtggttca ccgtttaccc ggacgtcggc
    28201 aatctcagcg cctggggcaa tgatgttccc gccgaactga aactgggcat cgaccgtatc
    28261 gccgccatcc acctgaaaga cactcaaccc gttaccgaac atagccccgg acagttccgc
    28321 gatgtgccgt ttggcgaagg ctgtgtcgat tttgttggca tcttcaaaac gctgcatgag
    28381 ctgaactatc gcggttcttt cctgattgag atgtggaccg aaaaagccaa agagccggtg
    28441 ctggagatta ttcaggcgcg gcgctggatt gaagcgcgta tgcaggaggc cggatttata
    28501 tgttagagca actgaaagct gacgtgctgg cggcaaacct ggcgcttccc gttcatcatc
    28561 tggtgacgtt cacctggggc aatgtcagcg cgatagacga aacgcggcaa tggatggtga
    28621 ttaagccttc cggcgttgag tatgaggtaa tgaccgccga cgatatggtg gtggttgaga
    28681 tagccagcgg caaggtggtg gaaggcagca aaaagccctc ttccgacacg ccaacgcatc
    28741 tggcgcttta ccgtcgcttt gcggagattg gcggcatagt gcatacccac tcgcgccacg
    28801 ccaccatctg gtcgcaggcc ggacttgatc tccccgcctg gggcactact cacgccgatt
    28861 atttttacgg agccatcccc tgcacgcgcc tgatgaccgc agaagagatc aacggtgagt
    28921 atgaatacca gaccggcgaa gtgatcatta aaaccttcga agagcgtggg ttgaacccgg
    28981 cacaaatccc ggcggtactg gtgcattctc acggcccgtt cgcctgggga aaaaatgccg
    29041 ccgatgcggt gcataacgcc gtggtactgg aagagtgcgc ctatatgggt ctgttctcgc
    29101 gccagcttgc gccacagctc cctgcgatgc aaaacgaact gctggataag cactacctgc
    29161 gtaagcatgg ggccaatgcc tattacgggc agtaatccct cacgccgggg cttcatcgcc
    29221 ccggcactac gaattgatat gttccttgct gtaacgccgc ttccacgctg ctggcgttaa
    29281 gccggtgtgt ttgcgaaaaa tctgccggaa atagccgacg tcattaaacc cgcacaacgc
    29341 cgccacttcc gtgagcgatc gcgcttcgct aattaacagc ttttcggctt cgcgcacccg
    29401 ctggcggtgg attgcttctg ttaacgtcag atgaaacacc cgccgataga cgcgcccaag
    29461 ataatcggca ttgcagtaca gctcgcgggc taaaagcgcc gtggaaagcg ggcggtgata
    29521 ttgcgtgcga atcagtttct gcgctttcca ggcaagggtg gtgcctgcac catcggcctg
    29581 ttcatcaaga cgcgcgtcac gggtgagctg ctgcaaaatc agcagcacaa taaactccag
    29641 cgccggagtg cgttgatgct tttcctgttc gcttaagaac tggcgaaaca aggccaccag
    29701 cacctgcgga tcggcaacct tagtttgttg cggcacgcta agatgagtga gccagcgcgg
    29761 atcgttgtcc ggcgctttaa cctcaaaatg cagccagtaa aatttgagat cggcgggaaa
    29821 atcctccacc ccaacatgct gtcggttcgg ccagagtaat aaactctctc ccgcatcgac
    29881 cagaaatatc cgttcatctt cctgaatcgt taatcgacct ttttcgacga agataatttc
    29941 ccatgactcc agccgccttg caggatgcct ccctacgcca cgggaaataa ataatccacc
    30001 attttgcact ttaatcggta acgtcatgga taattcgagc attgatatgt aatttcccgc
    30061 ttattcatta atgattaaac acattacctt ttgattatta atcaaaaaca ttaatttcac
    30121 ctgatccatt tcacactttt aaattcaggt cggaatcgtc accttttttt actcttttct
    30181 cgccttgttg atattcccgt tcacggcaga attaatcagg tcattgcaat aaaactaaaa
    30241 attgctccct attcacgagg aatatctatg acctctacac cgattaccac cgctgatatc
    30301 gctaaaggta aaattgacga tgcgttatct gtacgggaaa aaataggcta cggcctgggt
    30361 gacgcaggcg gcaccgtaat aacttgcctg atcatgaact ttctcacctt tttctacacc
    30421 gacgtttttg gcttaactcc ggcgctggtt ggcacgctgt ttattgcgct acgcgtgttt
    30481 gatgccatct ccgacccggt gatgggcgtc attgccgacc ggacgcaaag tcgctggggg
    30541 cgctttcgtc cgtggcagtt atggattgcc attcccatcg gcattatcgg catcctgaca
    30601 ttcaccgtgc cagatgccag catgggagta aaaatcgcct gggcgttcgg tacttacctg
    30661 ctcctttcag tcggttatac agccatcaac gtaccgtact gcgcgctgat caacaccatg
    30721 accacccgcc acaatgaagt gatctcctgc cagtcctggc gattcgttct ctgcggcgta
    30781 gcgggatttc tggtttcggt aggcttaccg tggatggtag atctcttcgg tcagggcaac
    30841 gctgcacgcg gctatcaact tggcgtcggc gtattgtgcg ccattgccgt ggtgatgttc
    30901 ctgtgctgtt tcttctgggt tcgtgaacgg gtgccactct ccacaatggg gaaatttacc
    30961 ctgcgcgaac atcttgccgg gctgcggaac aacgaccaac tgctgctgat gctggtcatg
    31021 tctttcctgc tgattaacgt ctttaatatt cgcggcggtg ggtatatgta cttcattacc
    31081 tacgtcttac aaggcagtac gggctacacg tcgctgttct tcaccatggt caccttcgcc
    31141 tccattatcg gctcggtgat tgtcagccca ttaacgcggc gtttcgatac cgtcaaaatt
    31201 tactactaca ccaacctgct cctcgctgca ctggcggtgt tgatgtggtt cctgccctcc
    31261 agcccggctt atcaaacgct gtggctggtg gtgatcctcg gtaatggcgt gattcttggc
    31321 ttcacattgc cactgcactt ctcattgatg gcctttgccg atgactacgg cgagtggaaa
    31381 acccgcgtac gttcttccgg catgaacttc gccttcaatc tgtttttcat caagctggcc
    31441 tgggcctcca gcgccgggat catcagcctg ctgtttattt ttgtcgccta ccagccaggc
    31501 gtggaaaacc agaccgccag ttcgcttggc gggatcgcgg cgatggaaac attgctgcct
    31561 gcgctgttcc acctgctgct ggcgatggcg atccgctttt gcaaactcaa taaccctatg
    31621 atgtcacgca ttgctagcga cctgcgtcag cgtcatgtac agccttaagg agcacaagat
    31681 gaacatttcg gaagtcgatc tgcataaact gacggtcagc gatccgttcc tcggtcagta
    31741 ccaacaactg gtccgcgacg tggtgattcc ttatcagtgg gatgccttga acgatcgtat
    31801 cccagaagcg gaacccagcc atgcgattga aaactttcgc attgccgccg gacttcagga
    31861 gggtgaattt tacgggatgg tgtttcagga cagcgacgtc gccaaatggc tggaagcggt
    31921 agcctggtcg ctgtgccaga agccggacgc cgaactggaa aaaaccgccg acgaggtgat
    31981 tgaactgatc gcttccgccc agtgcgaaga tggctatctc aatacttact ttacggtaaa
    32041 agcgcccgaa gaacgctgga gcaatctggc ggagtgtcat gaactttact gcgccggtca
    32101 tctgattgaa gccggagtcg ccttcttcca ggctacgggc aagcggcgct tgctggaagt
    32161 ggtttgccgt ctgaccgatc atatcgacag cgtatttggt ccagatgaaa gtaagttaca
    32221 tggttatcct ggtcacccgg aaattgaact ggcactaatg cgcctgtatg aagtgaccga
    32281 agagccgcgc tacctggcgc tgacgaacta ttttgtcgaa cagcgtggtg cgcaaccgca
    32341 ctattacgac caggaatatg aaaagcgcgg gcagacctcg cactggcaca cctacggccc
    32401 ggcgtggatg gtgaaagaca aagcctacag ccaggcacat ttgcccatcg cacagcagca
    32461 aaccgccatt ggtcacgcgg tacgttttgt ctacctgatg accggcgtcg cgcatctcgc
    32521 gcgtttaagt cacgatgaaa gcaagcgtca ggactgcctg cggctgtgga acaatatggc
    32581 ccagcgtcag ttatatatta ccggcggcat tggctcacag agcagcggtg aagcgttcag
    32641 cagcgattac gatctgccga atgacacggt atacgctgaa agttgcgcct ccatcggcct
    32701 gatgatgttt gcccgacgaa tgctggaaat ggaaggcgac agtcaatatg ccgatgtgat
    32761 ggagcgcgca ctgtacaaca ccgtgctcgg cggcatggcg ctggatggca aacatttctt
    32821 ctatgtgaat ccgctggaag tacatccaaa atcgctgaaa ttcaaccata tctacgatca
    32881 cgttaagccg atccgccagc gttggtttgg ctgcgcttgt tgtccgccaa atatcgcccg
    32941 cgtgctaacc tcgattggtc attatctcta cacgccgcgt gaagatgcgt tgtatatcaa
    33001 catctacgca ggaaacagca tggaggtgcc ggtagaaaat ggcacgctgc gcctgcgggt
    33061 tagcggaaac tatccgtggc aggaacaggt gacgattgcg gttgaatcac cccagccggt
    33121 gcgtcatacg ctggctttac gtctaccgga ctggtgcata cagccgcaga tcatattgaa
    33181 tggggaagag gtcgagcagg atattcgtaa agggtatttg cacattaccc gtgaatggca
    33241 ggagggcgac acgctgaatc tgactttgcc aatgccggta cgtcgcgttt acggtaaccc
    33301 gctggtgcgt cacgtcgccg gaaaagtggc gattcagcgc ggcccgctgg tgtattgtct
    33361 ggaacaggtc gacaacggcg agtcactgca taatctgtgg ctgcccgccg atgcgccatt
    33421 tacgacattt gaaggtaatg gattgttccg ccataagatc ttaatccagg caccgggtta
    33481 ccggtatgaa cagagcaatc cagagcagca accgctgtgg cattacgaca gcgcgccagc
    33541 caaacgccag ccgcaaattc tgacgtttat cccgtggttt agctgggcca accggggtga
    33601 aggcgaaatg cggatctggg tgaatgagga aaccagtgcc atccgtaggt aggataaggc
    33661 gctcgcgccg catccaacgc atggtgcaca ttgccggatg cggcgtgaac gctttatccg
    33721 acctacaaaa gcatgcaaat tcaatatatt gcatgagcta tgtaggcatg ataagacgcg
    33781 aaagcgtcgc atcaggcaat gaatacccga tgcgaccagc ttcttacatc agaacagccc
    33841 caacggttta tccgagtagc tcaccagcag gcacttggtt tgctggtaat gctcaagcat
    33901 cattttgtgg gtttcacggc caatgcctga ttgtttgtag ccgccaaacg ccgcatgtgc
    33961 cgggtaagcg tgataacagt tggtccacac gcgccctgcc tgaatgccgc gccccatctt
    34021 ataggccaga ttaccgttgc ggctccagac gcccgcgccc agaccatatt gcgtatcgtt
    34081 cgccagctcc agcgcttctt ccatcgtttt gaaggtggtt accgccagca ccgggccaaa
    34141 aatctcctcc tggaacacgc gcatattgtt ctgaccaaac agaatcgtcg gttcgaggta
    34201 gtagccgtct ttcagttcgc cttccagcag cttacgtcgc ccgccggtga gcacatcagc
    34261 gccctctttt ttaccgatat caatgtagtt gaggatggtt tccagttgcc cgtaagaaac
    34321 ctgcgcgccc atttgcgtca cgctgtcgag cgggttaccg ctgcgaatgc tttcgacacg
    34381 gcggatagcg cgttccataa agcgttcgta gatagactcc tgcactaaag cacggcttgg
    34441 acaggtgcaa acttcgccct ggttaaaggc aaacagtgca aagccttcca gcgctttatc
    34501 gaaaaaggca tcttcttcat ccatcacatc agcaaagaag atatttggcg atttgccgcc
    34561 cagctccagc gtcaccggaa taatgttttg cgtggcgtat tgcataattt gttggcccac
    34621 ttccgttgag ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca gatattcgcc
    34681 aatttcccca cctgcgccat tgaccacgtt caccacccct ggcggcagta aatcaccgac
    34741 gatttccatt agcagcagta cagaaagcgg agtaagacgt gcgggtttca gcaccacaca
    34801 gttgcccgcc gccagcgcgg gggccatttt ccagctcgcc atcagcagcg ggaagttcca
    34861 cgggataatc tgccccacca cgcctaacgg ttcatggaaa tgataggcca cggtttcgct
    34921 atccacttca ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat agcgaaaatg
    34981 atcaatcgcc agcggcacgt cggcagcact ggtttcgcga atgggtttgc cgttatccca
    35041 ggtttctgcc gtcgctaaaa gttcaaggtt ttgttccatt cgatcggcaa tcttaaacag
    35101 aatcgccgcg cgatcctgca ccgaggtgtg cgcccattta tctttcactt tatgcgcagc
    35161 atccagcgcc agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca gtagctgccc
    35221 ggtcaccggc gtcaggttct ggtaatactc gccatcggca ggcgctaccc attcgccacc
    35281 gataaagttg tcgtagcggg cttttaattt gagggggaaa ccatactcgc cgggcttaat
    35341 ctgtgctgaa gggggattat tggtcatgat cgtctccttg cggtgtgagg tataacaagg
    35401 gtagacgtga ctggcgaaat cttcgccagt cggtaacagc tttacgacgg ctatcacgaa
    35461 tttacgggca aaagctacag cgggtagtgt gcgacgtaca ctgcttgctg gttattaatt
    35521 cttttctctt gcgcgaatcc ttctttaaca attgtccgta aatcagctcg agccgtatta
    35581 tcagagacgc ccaagctcgc gctaatttca gcaactgtgt actctttccc cggattggcg
    35641 agcatcacat tcaggagcgt tacctggcgc ggattcaggc gtttaagcac accagagcta
    35701 aacagcacac tatcgagttt tgcgcttcgt tgtgtaattt catcgatatg ctgctgccag
    35761 ttttgcagag cacgtttaat gacacccgcc tgatattcaa ggaaataagt cagatccata
    35821 ccatctgatt cagtgtactg gtagctggcg gcatatttga ccggtgcagc atgaagcaaa
    35881 cggctgatgg aaatatattt aaagacatcg tagcccgatt tcagcatata ccagtaaaac
    35941 agcgcccgac tggtgcgccc gttgccatcc cgaaacggat gttcatgggc cagcataaaa
    36001 tgcagaatgc aggcacggac caacggatga atataccctt catggctgtt taaccactgg
    36061 caaacttttt ccagccgttc cggtaacaat gccgcagcgg gcggttgatg cactatattg
    36121 ccatcatagt cggcgatcac aacatcatct gtttcacgaa attctcccgg acgatatttc
    36181 gcgtcattga ttccacccat accaatggca tgtaactggc gaataagcgc aggtgtcagc
    36241 ggttctgcga gtaagtgcgg aatttccgcc atcagccggg catttcccgc aatcatatgc
    36301 tcgtcttcag tgcgtggcga ccgccctgtt tccagcattt ttctcgccac cagcgttgta
    36361 gtattcgcac cttccagctg ggagctggtg atcggctctt ccaggcgtaa ctggcttaac
    36421 tcggctccgg ccccgcgtaa ccgttcagtc tgatctttca gcgccagcgc ggtagcacgt
    36481 ttgtcgacaa gctcgcacgc ttctgcaatc accggggtaa tgttgaatcc ggcctgttca
    36541 cccgcttcat tgcaataatt aattcgttgg atcgccgcgt tgcgcgccag acgggtaagc
    36601 gtccacgcca catcgacgtt ttctcctttt ttgatgcggt actgcaactc atcaaaaggc
    36661 agataacgtc ctttttcatc ggtaacccgg ttaaagcgca ggtgggccag gagttcctca
    36721 cccgataagt gtgataaagg aataagtccg ggaggtgatt tgatagccat gtgcttatct
    36781 caaaaaagcg caatattgag atagtgtata gcaggaaaga gaaactatct caaaaaaagc
    36841 aagttttgag atagttgaaa aagcggctga caactcgcca gccgttgtgg gaatgatgat
    36901 tacatcgctg cgcgataaat cgccacaatt tcttcgtgag ttgcctggat cgggttagta
    36961 aagccgcagg catctttcag ggcattagtc gccagaaccg cgaaatcttc ttctttcacg
    37021 ttcaggtcgc gtaggcctgc cgggatatcc actttcttcg ccagttcacg gatggcgtta
    37081 atgcaggctt cagcaccttc cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg
    37141 gcacaatcac gcagacgtgc agcggcgact ttgctgttga atacctgaac atgcggcagc
    37201 aaaacggcgt tacatacacc gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc
    37261 gcatgaacat aacccagaga agcattattg aacgccatac cggcgaggaa ctgggcataa
    37321 gccattgctt cacgcgcttt cgcattactg ccatcttcaa cggctaacgg caggttttcg
    37381 gcaatcatgg tcacggcttt cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa
    37441 acatacgctt cgatagcgtg cgttaaggca tccatacccg ttgcggcggt cagtgacttc
    37501 ggcataccga tcatcagaga ggagtcattg acagaaagca gcggagtgac atgtttatcc
    37561 acaatcgcca ttttgatgtg acgcgcttcg tcagtgatga tgcaaaaacg ggtcatttca
    37621 gaggccgtac ccgccgtggt gttgatggcg atcatcggca gctgcggttt tgcagagcgg
    37681 tcaacgcctt cataatcgcg gatgtcaccg ccgttggctg caaccagcgc aatacctttt
    37741 gcgcagtcgt gtggagaacc accgcctaag gaaatcacgc tatcgcaatt attttcttta
    37801 agtaatttca aacctgcagc gacgttttcc gtggtgggat taggttgggt gccatcataa
    37861 ataacgctaa aaatattgcg ttcttccagt gctttttgca catcacccgc catacccaat
    37921 ttcgttaaca tattgtcggt gacaattaag gtacgggtaa atccataatc tgccatcata
    37981 ttcattgcat cattcaatga atcagcacca ataacattca cggaaggaat aaaaaaggtt
    38041 gaagctgcca taacacactc ctgaaagtgt aaaaaagggc gggcagctta ctgagaattt
    38101 tcatttgaaa atatgatcaa tattaccgtt tattaaaata tttttaataa aaagcacgtt
    38161 acattcatca aataacgtgc cttcttaaca ttaataagaa attttgtatt aattcatatt
    38221 tattatgaat taatcatttc cttatttttc cggaaataat aatgcgtcgc gcaataaatg
    38281 atcatttcca cgacgacgcg taaagccaat gcggtcaaaa tattccagaa tctgaattgc
    38341 cagcttgcgg cctacgccta agcgatcgcg gaaatccgcc gcgcaggttg aaccacattc
    38401 ctgatcgaga tcgcggatca tattggcaaa ttcgacaatc cgatcgttac ggtaataacg
    38461 atctttgacg atcgcagtaa ttatcccctg ttgggccgcc tggcgtagcg tcaggcgcat
    38521 tgcctgctcg tcggttcccg tctcttttgc caggtcacgc acccaccatg gctcgtcacc
    38581 aaacagcggc tctgcttttt gccagatggc ctgctgctct tcgctgaagc ccgctttatg
    38641 atccggcaga tgcagccagc cgtgatggct gtggatgtcg ccgctttcgc gcatcttttc
    38701 aatcagcaac agtaccagcg cttcatcttc cattggcaac gccatacgtc gcagacgttc
    38761 gcgcccagga ccaggttcat cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat
    38821 tttccgctgc cagcgggcgg caactggcgc attcaacaag ctgtaaccag cctgaatata
    38881 gccaggctgt tgcagcagtt cgcgcatccc ttcgccgttg agctgccgtg cccaggcgaa
    38941 atccgcaagg ttaaccgcgc cgcgttccag atgaacagat aacgcatcgg catcgctctg
    39001 cgcccgtgca agagacgcca gccattgcag atattccggc ttacgcttac cgcgacgcgg
    39061 cgggttaagc atcaccacac gcgcccccgc cagcgtgttg cgggccgaga tatcgcgcag
    39121 cactatgcga tcgttatccg ccagccacag cggcgtgtcg aacaccagct cggcaaggtt
    39181 atcttccagt agcgaaacac gtcccgtaac gtggctggcg gcgtggtgaa tatgcagcgg
    39241 ctgccactgg gtcagcggtg tatgggtttg aagctcgaca atcacccgtg tgaacggctc
    39301 cggcggcaca tcggcaagca gccagtcgcc acggttaatc tgctcttttt ccgcatcacc
    39361 cgcgatgtta agcgcgatac gctgcccggc atgggcagtt tctgttggct gattttgcgc
    39421 atgcagcgca cgcacgcgca tcggtttatt tacaccagtc agccagagtg aatcgcctac
    39481 tttcacttcc ccgcttaacg ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa
    39541 tgcgcggtcg atcgcgaggc ggaaactatg ttggctggcg tgttcacgtt ctggtaactg
    39601 aagcagatgc tcgcgcaggg catcaattcc ccgaccttcg gttgctgcgg tgataaacag
    39661 ttttgcctca gcaaaaccgt attcccgcag cacctccttt acctggcgtt caacctcatc
    39721 aacacgcgct tcgtccacgc gatcggcttt ggtcagcgcc actgtcagca tcgggttacc
    39781 ggtcagctgc aaaatcgcca gatgctcacg ggtctgtgcc atcacaccgt catcgcacgc
    39841 caccaccaac agcgcgtgat cgataccacc aacgcccgcc agcatgttgg aaagaaactt
    39901 ttcatgaccg ggtacgtcga taaaaccagg cacgcgacca tccggctgcg gccagtaggc
    39961 atagccgaga tcgatggtca tgccgcgctt tttttcttcc ggcagacggt cggcatttac
    40021 gcccgtaatc gcctgcaaca aggttgtttt gccgtggtca acgtgtccgg cagtcgtaat
    40081 aatcatttca acaacatctc caaaaaccgt tgctcatctt caaggcagcg taaatccagc
    40141 cacaatcgtc cgtcataaat acgaccaatc accggcactg gcaattcacg ccagcgggcg
    40201 gctaatgact caaggtggct accgcgtcca tcatgggggg taaacgttaa tgccgcgctc
    40261 ggcaggcgat caaccggcag cgaaccactg ccgatctgcg aaagacatgg cataacctgt
    40321 accgcaaact ccgcgccgta atgtgcggca aggagagcct gtaaacgttg tgcctggatt
    40381 tgaatgacct ctgcgctgcg ggtaagcagg cgcagggtcg gtaatttttc actcagagct
    40441 tcagggtgta aataaagacg caacgtggct tccagcgccg cgagggtcat tttatccgcg
    40501 cgtaatgcac gcttcagcgg gtggctttgc aggcgggcga tcatttcttt tttaccaaca
    40561 ataattcctg cctgcggccc gcctaacagc ttgtcgccgg agaagctcac cagactgacg
    40621 cccgccgcaa tcaactcctg cggcattggc tctttcggca aaccgtactg gctaagatcg
    40681 accagcgagc cactgcctaa atcagtcacc actggaacat ccagttcttt gccgagcgcc
    40741 accagttccg cttcatctat cgctttggtg aacccctgaa tgctgtagtt actggtatgt
    40801 actttcatca acagtgcggt attttcattc accgcctgac gataatcatt cgcgtgcgtg
    40861 cggttggtgg tccctacttc gtgtagggtg catcctgcct gacgcataac atcgggaata
    40921 cgaaacgcgc cgccaatctc caccagttcg ccgcgagaaa ccaccacctc tttcccgctg
    40981 gcagtggccg ccaacatcaa taacaccgcc gccgcattgt tattaacgat acaggcatct
    41041 tccgcccccg taatacggca cagcagctgc gccagcgccc gatcgcgatg tccacgtccg
    41101 gcgtcgtcca gatcgtactc gagggtcact ggcgaacgca tagccttcgt aacggcttcc
    41161 accgcggctt ccgcctgtaa agctcgccca aggttggtat gcagcacggt tcccgtcagg
    41221 ttgatcaccg gacgcagcgc gctttgcgcc tctttcgtca accgggcatc gacttcttgc
    41281 gcccagtttt cacaccacgc gggcagcgtc tggctgtcac gaatcacttc tcgcgcttcg
    41341 tcaagcatct gacgcagcaa ttccaccacg cgggtgtgac cataagtatc acgcaaagaa
    41401 aggaaggagc tatcgcgcaa taagcgatca atagccggaa gttgactata gagggaacgc
    41461 gtttcggttg tcataggaaa cctggctgat caaggccctc tcacacggag aagggcgttt
    41521 aacataacca cggattgtaa cgtgagatgg gtcaggagga catatcgcgc atcaagcctt
    41581 tggcggttcg gtgcgagcaa aactttcgcg gctaaacagg ttttcgacca gtttgactaa
    41641 gtgtgggcgg tcgacacacc agcctggcgc aacgcggcgg aaattgagat atccgaccgc
    41701 acaggcaatg gcgatagtcg ccagattgac cgtatcggtt ttgagcgtgc catcgaccag
    41761 atatccttcc agcacatcca gactgcggtt gattttctca cgctgacgta acaattcatc
    41821 ttcagactgc tgcgccgccg gacgcgcctg ttcacgcacc gataccagcc cggcatccat
    41881 aatgccatcc gccagcgcct caattttgcg cacccgcaac gactccagcg gatcacgcgg
    41941 caacatcgcc ggagcaacat tcattaattc aatatattca gcgatgatcg gcgaatcaaa
    42001 ccagcattcg ccctcttcgg tcaccagcac cggcactttt cctaacgggt taaattgcgc
    42061 cacgccgttg tccgcgttat agggcagttc attaataaat tcgaacgtta tgcccttttc
    42121 taacaacaga atagaaagtt tgcgtacaaa cgggctggtg tagctaccga cgagtttcat
    42181 gccgagtcct ttgtgcgagg aaaaatatca gtatggctca ggctcgcgca aaacacaggt
    42241 gatacaagtc tcaatgatcc aaataaagat aatgcatcca actggtcatt cttagcagca
    42301 ctttccgcat caccgaaaca tggctgaaat ggtcggagta aactgccacc tgggcgtaga
    42361 tgccgtcggg taaggcatcg atatcagcgt tagggtccag ctcaatggtt cccagcacac
    42421 cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc
    42481 ctggcacgac aggtaaaata ctggtcagtt tgccgtgaaa cacctgccca ggtagcgcgt
    42541 taaacaccac ttccgcatca tcgccgggtt tcagacgtaa cagcgagttt tgccgaaatt
    42601 gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc agcggcaagg
    42661 cagcggcgta tgtacctggg cggatcagta cctgagtgac gtagccattg ctcggtgcgc
    42721 gaatgacggt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt aagctcacaa
    42781 tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg
    42841 ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt
    42901 cacgttccga gaacggattc accgccgcct ggctgccttt cagataacgt tgataatttt
    42961 taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagtt
    43021 gcgcgcgcag cgtctttata ttatgcgtcg ccgtcatcag gtctgcctga agtctgtcaa
    43081 ctcgcgcctg gtaacgaacc ggctcgagct taaaaagcac ctcgcccttc tgaataagct
    43141 gattattttt gtcggtgact tcagtaacaa ttcccgtcac ctgtggcgtg atggggatcg
    43201 ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaagatca
    43261 aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac tgatttaccg
    43321 gaatgcggaa gattttaaag accgcccacg ccagcgccac gtaagttaaa acaatcaata
    43381 gatccatatt tagatctccg gaaacgtgga atagtcagtc tttttctcgg cggcgagttg
    43441 atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag tcgctttctc
    43501 ttgcgacgca acatgtgact gcatgcccca gccgcgctcc ggttgataga gcgtcgccca
    43561 gatccacagg aacggccaga taacatgtag agtaaacagg ctcacccagc ccgccgtatg
    43621 aatagcgtcg gcatggggat ggttgcgctt tttggcaatc agataaggga tgtcatgtat
    43681 cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata
    43741 gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg gctggcagcc
    43801 agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac
    43861 caaagaataa ttttaagagg tgatgagttg cttagttaca taacgatttc atgacgaagg
    43921 cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat
    43981 cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt taaatgccat
    44041 ttttagcgaa aatcgccgcc ttgttgcttt tttacacaag cattttgtga tgagcatcac
    44101 gtcaattacc tctctacccc ctacatttat gtgactgata tcacacaaaa ggccgccgac
    44161 tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg
    44221 ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg
    44281 gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca
    44341 acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta
    44401 ttccaacagg gtggttaccg aacgaaacgc tggcgaagct ggtcgggccg atgatcactt
    44461 atctcctgcc gctgctgatc ggttataccg gtggtaagct ggttggcggc gaacgtggcg
    44521 gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc
    44581 tcggctctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg
    44641 tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca
    44701 tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt
    44761 ccaaaatgct ggcggcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt
    44821 ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct
    44881 tctcgccgct gggtattcag cagtcccatg aactgggtaa atccatcttc ttcctgattg
    44941 aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta
    45001 gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctggggggt atccacgaaa
    45061 tctacttccc gtatgtgctg atgaatccgc gtctgattct ggccgttatc ctcggcggta
    45121 tgactggcgt attcacgctg actatcctgg gcggtgggct ggtttctccg gcatctccgg
    45181 gttctatcct tgctgtactg gcgatgacgc caaaaggtgc ttacttcgct aacatcgcgg
    45241 gtgtgtgtgc ggcgatggca gtttccttcg ttgtctctgc cattctgctg aaaaccagca
    45301 aagtgaaaga agaagatgat attgaagctg ctgcacgtcg cgttcaggaa atgaaagctg
    45361 agtctaaagg tgcgtctccg ctgtctgcag gcgatgtgac taacgacctg agccacgtcc
    45421 gtaaaatcat cgttgcctgt gatgccggta tgggttccag tgcgatgggt gcaggcgtgc
    45481 tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac agcgcgatca
    45541 acaacctgcc gccagatgtg gacctggtca tcactcaccg tgacctgacc gaacgcgcta
    45601 tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac agcggcctgt
    45661 acaccagcct gaccgaacgt ctggttgctg cccagcgcca tactgaaaac gaagtgaaag
    45721 tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc aaactgggcg
    45781 cggagaacat cttcctcggt cgcaaagcgg ccaccaaaga agaagcgatt cgttttgctg
    45841 gcgagcaact ggtgaaaggc ggttacgttg agccggaata cgttcaggcg atgctggatc
    45901 gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg
    45961 aagcgaaaga tcgcgtactc aaaacgggcg tcgtgttctg ccagtacccg gaaggcgtgc
    46021 gcttcggtga agaagaagat gacattgccc gtctggtgat tgggattgct gcccgtaaca
    46081 acgagcacat tcaggtgatc accagcctga ccaatgcact ggatgatgag tccgtcatcg
    46141 agcgtctggc acacaccacc agcgtggatg aagtgctgga attgctggca ggtcgtaagt
    46201 aatccattcc caccctctcc gcatgaagaa ggtggggcta attgcttgat gcgcttcgct
    46261 tatcaggcct acaacataaa gcacaatttg ttttgtcggt cggataaggt gtttacgccg
    46321 catccggcag tcggtgcaca atgcctgatg cgacgctgac gcgtcttatc agacctacag
    46381 gtcagtgcca aacacgtagg ccggataagg cggtttacgc cgcatccggc aaaaagcccc
    46441 tctcctcacg gagagggttt gggtgaggga aaagcctcac cccagccctc tcgggtaaaa
    46501 acattgatga aggttaatac tatgaaagca ttacattttg gcgcaggtaa tatcggtcgt
    46561 ggctttatcg gtaaactgct ggcagacgca ggtatccaac tgacgtttgc cgatgtaaat
    46621 caggtggtgc ttgatgccct gaatgcccgt catagctatc aggttcatgt ggttggtgaa
    46681 accgagcagg tagataccgt ttctggcgtc gatgccgtca gcagcattgg tgatgatgtc
    46741 gttgatctga ttgctcaggt tgatttagtc actaccgccg ttggcccggt cgtgctggaa
    46801 cgtattgctc cggccatcgc caaagggctg gtgaaacgta aagaacaagg taatgaatct
    46861 ccgctgaaca tcatcgcctg tgaaaacatg gtccgcggca ccacgcagct gaaaggccat
    46921 gtgatgaacg ccctgcccga agacgccaaa gcgtgggtag aagaacacgt tggctttgtt
    46981 gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg caactaacga tccgctggaa
    47041 gtgacggtag aaaccttcag cgaatggatt gtcgataaaa cccagttcaa aggcacgctg
    47101 ccaaacatcc ctggcatgga attaaccgac aatctgatgg catttgtcga acgtaaactc
    47161 ttcaccctga atacaggtca tgctataacc gcgtacctcg gaaaactggc gggtcatcag
    47221 accattcgtg acgcaattct cgacgagaaa atccgcgcgg tggtaaaagg ggcaatggaa
    47281 gaaagtggtg cagtattgat caagcgctac ggctttgacg ctgacaagca tgcggcatac
    47341 atccagaaaa tccttggtcg tttcgagaac ccgtacctga aagatgatgt agaacgcgta
    47401 ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc tgatcaagcc actgctcgga
    47461 accctggaat atggtctgcc acataaaaac ctgattgaag gtattgccgc tgcaatgcac
    47521 ttccgcagtg aagatgatcc gcaggctcag gaactggcag cgctgattgc cgacaaaggc
    47581 ccacaagccg cgctggcaca gatttccggt cttgatgcca acagcgaggt tgtatccgag
    47641 gcggtaaccg cttataaagc aatgcaataa tggtggacca ggcgcaggat accctgcgcc
    47701 cgaataacag attgtcagat atgcaggcaa caatggaaca aacccaggcc tttgaaaacc
    47761 gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag ctttctgatc accgccgtcg
    47821 agcttttgac cgaggcggta aatcttctgg tgcttcaggt attccgcaaa gacgattacg
    47881 cggtgaagta tgctgtagaa ccgttactcg acggcgatgg tccgctgggc gatctttctg
    47941 tgcgtttaaa actcatttac gggttgggcg tcattaaccg ccaggaatac gaagatgcgg
    48001 aactgctgat ggcattgcgt gaagagctaa atcacgacgg caacgagtac gcttttaccg
    48061 acgacgaaat ccttggaccc tttggtgaac tgcattgcgt ggcggcgtta ccaccgccgc
    48121 cacagtttga acctgcagac tccagtttgt atgcaatgca aattcagcgt taccaacagg
    48181 ctgtacgatc aacaatggtc ctttcactga cagagctgat ttccaaaatc agcttaaaaa
    48241 aagcctttca aaagtaagta ctttcgctta cttctcctct acctgtttcg gacgataaag
    48301 ctgacgataa tactccagac gttgtagata taacggcacg ctttcatcag gtattccgga
    48361 cggaatcgcg ttacggggag gtagtttttt cagatactcc cggaacgcct ggctcgatgc
    48421 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
    48481 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttagatcccg
    48541 gtataaatga cattaaggaa aacgtgctca atcctcaata atcacctgct tctgttgact
    48601 atcttttttc tttaaatttc ttaactaatc aatgtattgg gtgatttttt aacgcattaa
    48661 tggttacaat ctggaactat tacaatgcca tcagttgtga acaaagcacc tggtcgcgca
    48721 tactagaggc tataaattta tctctatcag aagtcatcac atgaaagaag tcgaaaaaaa
    48781 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgt caccagcagg ccgatctatc
    48841 gctggttgaa gccgcagaca aatatgccga actggaaaaa gagaaagcca cgctggaagc
    48901 agaaattgct cgcctgcgcg aagttcacag ccagaaactc agcaaagaag cgcagaaact
    48961 gatgaagatg ccgttccagc gtgcaatcac caaaaaagag caggctgata tgggcaaact
    49021 gaaaaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
    49081 gggcctgcaa gagatgacag ggttctcaaa gactgcgttt taagttcctt tctcgtcgct
    49141 gtaactactg ccactcacga tttactcgct cctgctggca catgcaggag cctgtgccac
    49201 tcattaaata cgatgtcttt cttaccgtaa actgtttcag tttcccctcg agtttgaggt
    49261 atttttgcag gctaaaatct gaaaatttgt tgcgcaacaa caagtgatat ataaatcata
    49321 aaaataaata atcgaattat gatgcatgat tatttatctt tccccataaa cacagcaaat
    49381 ttcatcacaa cacatcattt atatgaatat aaaatctccc tgcgctcccc agcctaagac
    49441 actaaacatt tgacattgat tgataccgct cattaaaatt cgcgcgactc ataacattcg
    49501 ctacaaactg aaatcagttg tcatttaata aaacgtccgt aataccaatt atttcaataa
    49561 atctcttata agttttattg cgaatacata tataaaaaac attcatcaga agaatgaata
    49621 tggcgtaatt atcaacatat tggggttatg aaaaggaatt catcctatga gcatgaaaac
    49681 acttttaaac gtgttaacaa tattttcact aggtacatta acaggctgtg attatattga
    49741 aaaagcgagt ctgatcgatg atcttgccaa acagcaagag ttgcaaaaaa gtaaaataga
    49801 ggagcttgag aaacaacaag aacaatataa aaataaagca gaactgtttg aaaaacaaca
    49861 aaataatatc acaaacagca ccagaatact tgccagcatt gtaaagacag tcaaagaaaa
    49921 acaggacgaa tttgtcttca ccgaatttaa cccgctacaa acccaatact tcattttaaa
    49981 taacggctcg gtcggtttgg caggccgggt gctggctatt gatgcggttg aaaatggcag
    50041 tgtcattcgt atttcgctgg ttaacttatt aagcgttccc gtatcaaata ttggctttca
    50101 tgcgacatgg gggaatgaga aacccaccga tgccaaagct ctggcgaaat ggcaacaatt
    50161 actattcaac accaccctga attccactct gcaattaatg cctggtcaat ggcaagacat
    50221 taatctgaca ttaaaaggcg tctcgcctaa taacctgaag tatctgaagt tgtccatcaa
    50281 tatggctaat cttcagttcg acactgttca acctgctgaa acccggcagc ggaaaaacaa
    50341 aaaataaatg cagcttctgc gcttatatca aggaatagag agcatcaata atgaacaaaa
    50401 tatttaaagt tatctggaat ccggcgacag gcagttacaa cgtcgccagc gaaacggcga
    50461 agagccgtgg caaaaagagc gggcgcagta agctgttaat ttctgccttg gttgcaggtg
    50521 ggctgttatc gtcatttggg gcactggctg ataactacga ggggcagcca gtggattatg
    50581 gcaatggctc agcaggtgac ggctgggttg ctatcggtaa aaattcaaaa gcaaatacct
    50641 ttgtcaatgg ctctggtgcg agcactgcgc taggttatga cgcgatagct gaaggtgagt
    50701 acagctcagc tataggctca cgaactcatg cgattggtgg tgcatcaatg gcctttgggg
    50761 ttagtgcaca agcaataggt gacagaagtg tcgcgctggg tgcatcttca gtagcaactg
    50821 gcggtcgttc gatggctctt ggtcgttacg caaagacgaa tggttttaca tctcttgcta
    50881 ttggggacac cgcccttgcc gatggtgaaa aaactattgc gttaggaaat acggctcaag
    50941 cttacgaaat tatgagcatc gccctcggtg atcatgctaa tgcctcaaaa gaatattcaa
    51001 tggcgctggg agcaagtagc gttgcatctg cggctaacgc cattgccgta ggccgcgaca
    51061 gtgctgctaa cggcacagac agccttgcct taggcagact atctctcgcc agcgcagcaa
    51121 acgcgattgc tatgggtgca gagagcgaag ctgctgaaaa tgcgacggcg attggcttta
    51181 acgcggatgc gatcgggaag tccagcctgg cgctgggaga caacgccagc gctggtgaaa
    51241 ccaactccat cgctataggt caaggcagcg aggcaagcaa acttgattca attgcgctgg
    51301 gaagtaacag caggtcagcg ggtgagaacg ccattgcact gggcaacaac agtaatgcgg
    51361 ggggcaaaaa cagccttgct ttcggtttta acaccaccgc aaatggtgat aacgcagtcg
    51421 ccattggtgc aaattcttcc gcaggtgctg acaataccgt gtctgtaggt agctctagtt
    51481 taaagcgcaa aattgtcaat atgggtaatg gtgacatcaa taatgtcagc tccgatgcca
    51541 tcaatggatc acaactttat gccatcagta agtctgtatc cgacaggctt ggaggatatc
    51601 atgatgatcc tgataatgtt ataaactctg acgggacgct taaagctccc acatattatc
    51661 tacaaagtgg ccaatacaat aacgtgggag aggctctcca gagcatcgac aataacacgt
    51721 tgcactggga cagcaaaagt aataaataca gtgccagcca tactgtcttt aatgcaaatg
    51781 gctcagttaa aagcacctcg gcgaaaaaca tcattaccga cgtcgctgat ggcacgattt
    51841 cagccaccag ttccgacgcc gttaacggtt cgcagttgta taatctgaaa caagatgcct
    51901 tgctatggga tggcactgcg tttagcgcca aacatggcac cagcaatact aacagcaaaa
    51961 tcaccaacgt tgcggatggt gctgtttcag cctccagtaa agatgccgtt aacggttcgc
    52021 agttgtatga tctgaaacaa gatgccctgc tatgggatgg cactgcgttt agcgccaaac
    52081 atggcaccag caatactgac agcaaaatca ccaacgtact ggcaggcaca gtatcttcca
    52141 ccagcaccga tgccattaat ggtacacaac ttcaaagcac tgcaagcacc attacgtcct
    52201 atttgggcgg cggcgcggca atgagtaatg acggcgtatt taccggtcct caatataata
    52261 ttggtggtaa gaattacacc aatgttggcg cagctcttac cgctctggac aatgacgccc
    52321 tgttgtggga tgcaaccgct aattcaaata ctggcgcatt cagtgctgca cacggaacac
    52381 tcaaaaccgc cagcaagatt accaacgttg caaatggtga catattagcc aacagttatg
    52441 atgctattaa tggctcacaa ctttatagtt tagctgattc atttacgtca taccttggcg
    52501 gtggtgctga tatcaacaat gcgggtgttc tcatagcccc gacctatact atcggttcga
    52561 aaacgtacaa caacgtaggt gatgccctta acgcgattaa ctcatcattc agcacctcaa
    52621 tcagtgatgc cctgctgtgg gattctacag cgaacaaatt cagcgccaaa cacggtggta
    52681 ccaatagcat tattactaac gttgcagacg gtacaatttc ctctaccagc agcgatgctg
    52741 taaatggctc gcaactctat gacacgagca agtacattgc tgatgccctg ggtggtgacg
    52801 cagaagttaa cgctgacggc accatgactg caccgactta ctctatcgct aatgaagatt
    52861 accacagcgt aggtgaagcc ctggatgcgc tcgatgataa cgcgctgctg tgggatgaag
    52921 acgcaggtgc ctacaacgcc agccatgatg gcaatgccag caaaattacc aacgttgcgg
    52981 caggtgatct ctccacaacc agtaccgatg ctgttaacgg ttcccagtta aacgcaacca
    53041 atattctggt tacgcaaaat agccaaatga ttaaccagct tgccggtaac accagcgaaa
    53101 cctacatcga ggaaaacggt gcgggtatta actatgtacg taccaacgac aacggcttag
    53161 cgttcaacga tgccagcgcc tcaggtattg gtgctacggc tgtgggttat aactctgttg
    53221 ccgaaggtga tagcagcgtg gccattggtc aaggtagcag cagctccgtt gataccggca
    53281 ttgccttggg tagcagctcc gtttccagtc gcgtgattgt taaaggttct cgtaacacta
    53341 gtgtaacgga aaatggcgta gttatcggtt atgacaccac tgacggtgag ttgctcggtg
    53401 cattgtcaat tggtgatgac ggtaaatacc gtcaaatcat caacgtcgcc gatggttccg
    53461 aagcccatga cgccgttacg gttcgccagc tgcaaaatgc cattggtgca gtcgctacca
    53521 cgccgactaa atacttccac gctaactcaa cggctgaaga ctcactggca gtcggtgaag
    53581 actcgctggc aatgggcgcg aaaaccatcg ttaatggtaa tgcgggtatt ggtatcggcc
    53641 tgaacacgct ggttctggct gatgcgatca acggtattgc tatcggttct aacgcacgcg
    53701 caaatcatgc cgacagcatt gcaatgggta atggttctca gactacccgt ggtgcgcaga
    53761 ccaactacac tgcctacaac atggacgcgc cgcagaactc cgtgggtgag ttctctgtcg
    53821 gcagtgaaga cggtcaacgt cagatcacca acgtcgcagc ggggtcggcg gataccgatg
    53881 cggtcaacgt gggtcaattg aaagtaacgg acgcgcaggt ttcccagaat acccagagca
    53941 ttactaacct gaacactcag gtcactaatc tggatactcg cgtgaccaat atcgaaaacg
    54001 gcattggcga tatcgtaacc accggtagca ctaagtactt caagaccaac accgatggcg
    54061 cagatgccaa cgcgcagggt aaagacagtg ttgcgattgg ttctggttcc attgctgccg
    54121 ctgacaacag cgtcgcgctg ggcacgggtt ccgtagcaga cgaagaaaat acgatctctg
    54181 tgggttcttc taccaaccag cgccgtatca ccaacgttgc tgccggtgtt aatgccaccg
    54241 atgcggttaa cgtttcgcaa ctgaagtctt ctgaagcagg cggcgttcgc tacgacacca
    54301 aagctgatgg ctctatcgac tacagcaaca tcactctcgg tggtggtaac ggcggtacga
    54361 ctcgcatcag caacgtctcc gctggcgtca acaacaacga cgcagtgaac tatgcgcaac
    54421 tgaagcaaag cgtgcaggaa acgaagcaat acaccgatca gcgcatggtt gagatggata
    54481 acaaactgtc caaaaccgaa agcaagttga gcggtggtat cgcttctgca atggcgatga
    54541 ccggtctgcc gcaggcttac acgccgggtg ccagcatggc ctctattggt ggcggtactt
    54601 acaacggtga atcggctgtt gctttaggtg tgtcgatggt gagcgccaat ggtcgttggg
    54661 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
    54721 gtattcagtg gtaatgatcc attaacgaat gggtaaacgc cacattgcct gatgcgctac
    54781 gcttatcagg cctacggggt gattcagcac attcctgtag gtcggataag gcgttcacgc
    54841 cgcatccggc aagataaggc actccgggtc aacaatctaa gggcaattct ctgatgagga
    54901 ttgccctttt ctttaccaga catctcccac cacaagaatt ggccctacca attcttcgct
    54961 tatctgacct ctggttcaca attccccaat taaaactcac atcaatgttg ccaacacgta
    55021 acatttagtt aaccattcat tgtcattacc cctacacaac acaattggca gtgccacttt
    55081 tacacaacgt gtgacaaaga gatgggcaac agactcatta cacggcgtgt gtggactgca
    55141 ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat atctggcttt
    55201 ccagcctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt aagctcaaac
    55261 tgaaaggata cgtcgccgcc tcatggacgg tggcaatcgc ccttgccgtg gctttgctgt
    55321 tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc ttctacgggt
    55381 tgtggcccat cgcgtggatc attattgcag cggtgttcgt ctataagatc tcggtgaaaa
    55441 ccgggcagtt tgacatcatc cgctcgtcta ttctttcgat aacccctgac cagcgcctgc
    55501 aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca ggctttggcg
    55561 caccggtagc aattaccgcc gcattgctgg tcggcctggg ctttaaaccg ctgtacgccg
    55621 ccgggctgtg cctgattgtt aacaccgcgc cagtggcatt tggtgcgatg ggtattccga
    55681 ttctggtcgc cgggcaggta acaggcatcg acagctttga gattggtcag atggtggggc
    55741 gtcagctgcc gtttatgacc attatcgtgc tgttctggat catggcgatt atggacggct
    55801 ggcgcggtat caaagaaacc tggcctgcgg tcgtggttgc gggcgggtcg tttgccatcg
    55861 ctcaatacct cagctctaac tttattgggc cggaactgcc agacattatc tcttcgctgg
    55921 tatcgctgct ctgtctgaca ctgttcctca aacgctggca acccgtgcgc gtattccgct
    55981 tcggtgactt aggggcgtca caggttgata tgacgctggc tcacacaggt tacacggcag
    56041 gtcaggtgct gcgcgcctgg acaccgttcc tgttcctgac cgccaccgta acactgtgga
    56101 gtatcccgcc gtttaaagcc ctgttcactt caggcggtgc gctgtatgag tgggtgatca
    56161 acattccggt gccgtacctc gataaactgg ttgcccgtat gccgccagtg gtcagcgaag
    56221 cgacagcgta tgccgccgtg tttaagttcg actggttctc tgccaccggt accgccattc
    56281 tgtttgctgc cctgctctcg attgtctggc tgaagatgaa accatctgac gctatcagca
    56341 ccttcggcag cacgctgaaa gaactggctc tgcctatcta ctccatcggc atggtgctgg
    56401 cgttcgcctt tatctcgaac tattccggtt tgtcatcaac actggcgctg gcactggcgc
    56461 acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggcttggg gtattcctga
    56521 ccgggtcgga tacgtcatct aatgctttgt ttgccgcttt gcaggctacc gccgcacaac
    56581 aaattggcgt ttctgacctg ttgctggttg ctgccaacac caccggtggt gtcaccggca
    56641 aaatgatctc tccgcaatct atcgctatcg cctgtgcggc ggtaggcctg gtgggcaaag
    56701 agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt atggtgggcg
    56761 tgatcaccac gcttcaggcc tatgtcttaa cgtggatgat tccttaatga ttgttttacc
    56821 cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg aaaaaaacct
    56881 ggaagcgggc atgaagttgc ccgctgaacg tcaactggcg atgcaactcg gcgtgtcgag
    56941 aaattcactg cgcgaggcgc tggcgaaact ggtaagcgaa ggtgtgctgc tcagtcgacg
    57001 tggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa acatcgtcca
    57061 gccgctgaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc tggaagcccg
    57121 ctacgccatt gaagccagca ccgcatggca tgcagcaatg cgcgccacac ctggcgaaaa
    57181 agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggatc tcgcctcaca
    57241 agcggacgtt cgttttcatc tggcgattgc cgaagcttca cataacattg tgttgctgca
    57301 aaccatgcgc ggtttcttcg atgtcctgca atcctctgtg aagcatagcc gccagcggat
    57361 gtacctggta cccccggttt tttctcagct gaccgaacaa catcaggcgg tcattgacgc
    57421 catttttgtc ggtgatgctg acggggcgcg taaagcaatg atggcgcacc ttagctttgt
    57481 tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc catgcacgaa ttacccgcct
    57541 gcctggtgac cataatgagc attcgaggga gaaaaacgca tgattatttc cgcagccagc
    57601 gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgtttcacta tatggatggg
    57661 ggggcatatt ctgaatacac cctacgccgc aacgtggaag atttgtcaga agtggcgctg
    57721 cgccagcgta ttctgaaaaa catgtctgac ttaagcctgg aaacgacgct gtttaatgag
    57781 aaattgtcga tgccggtggc gctaggtccg gtaggtttgt gtggcatgta tgcgcgacgc
    57841 ggcgaagttc aggctgccaa agccgcggat gcgcatggca ttccgtttac tctctcgacg
    57901 gtttccgttt gcccgattga agaagtcgct ccagctatca aacgtccgat gtggttccag
    57961 ctttatgtgc tgcgcgatcg cggctttatg cgtaacgcac tggagcgagc aaaagccgcg
    58021 ggttgttcga cgctggtttt caccgtggat atgccgacac cgggagcgcg ttatcgtgat
    58081 gcgcattctg ggatgagcgg cccgaacgcg gcaatgcgcc gctatttgca ggcggtgacg
    58141 catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cgcatgattt aggtaatatc
    58201 tcggcttacc tcggcaaacc gaccggactg gaagattaca tcggctggct ggggaataac
    58261 ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg ggatggcccg
    58321 atggtgatca aagggatcct cgacccagaa gatgcgcgcg atgcagtacg ttttggtgct
    58381 gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtgct ctcttctgcc
    58441 cgtgcactgc ctgctattgc agatgccgtg aaaggtgata tcgccattct ggcagatagc
    58501 ggaatacgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga caccgtactg
    58561 ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt agctaacctg
    58621 ctaaatctga tcgaaaaaga gatgaaagtg gcaatgacgc tgactggcgc gaaatcgatt
    58681 agcgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc ggcggcattg
    58741 gctccgatgg cgaaagggaa tgcagcttaa gggttagacg aatatctgct atcctgcccc
    58801 cgcactaagg gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata
    58861 ctggcaacat catccgtctt tgcgctaata ccggctttcg tctgcatatc atcgaaccga
    58921 tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg gctggactat cacgagttta
    58981 ccgccgttac gcgtcatcat gactatcgcg cgtttctcga agcagaaaat ccccagcgtc
    59041 tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg
    59101 attatctgat gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc
    59161 ccgctgaaca aaaaattcgt attccaatgg tgccggacag ccgcagcatg aacctgtcca
    59221 atgcggtgtc ggtcgtggtg tatgaagcct ggcggcagtt ggggtatccg ggagcggtat
    59281 tgagagatta gttactgtag gccggataga tgattacatc gcatccggca cgatcacagg
    59341 acatcagatc ccatcaccat actcaaacgt atggttaatg ccgttgaaat gctgatccat
    59401 atccattgac ggtttatcgc tatccggctt gccgacaatg cgtgccggaa cgccagcggc
    59461 agtagtgtgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg
    59521 cccaacttca atattgccga gaattttcgc gcccgcgcca atcatcacac cttcacgaat
    59581 tttcgggtga cggtcgccac cagatttacc cgtaccgcca agcgtcacag attgcagaat
    59641 cgatacgtcg ttttcaatca ccgccgtttc accaacgacg atgcctgtcg cgtggtcgag
    59701 catgataccg cgaccaattt ttgctgccgg gtgaatatca acctggaacg tcacagaaac
    59761 ctggttttgc agaaaaatag ccagtgcgcg acgcccctga ttccacaacc agtgaccgat
    59821 gcgatacgcc tgcaaagcat gaaaaccctt caggtatagc aacggggttg agtatttatc
    59881 caccgccggg tcacgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc
    59941 cgggtcggcg gcgtaggctt cttccaccac ttcacggata gcaatagccg gcataatggg
    60001 cgatgacagc ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt cgtgcttgag
    60061 tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc
    60121 agctttaata ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg
    60181 atgagataat gaccgacccg ttctgcgcgg gccgggtcat agtggtaaca aaggttccct
    60241 gggtttagtg gctgctgcgc tcgtccttgc gtgcacgacc tagtaaagtc aatgctgcct
    60301 cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcaa
    60361 cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccactt
    60421 gaccaatttt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc atgccaaaac
    60481 ggcggttacg cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc
    60541 ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag acgcgacatc tcagccagcc
    60601 cacgagtgat cagcgccgta cgcgcattcg caccaaaacc gataccgtcg gacatccccg
    60661 cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg cacaccgatg aaatccgggt
    60721 tgctgtaaac gcggaaactt ttgccacagt gcagcaattg ctggaggtca tcggcaaagg
    60781 tctggtccgt cgaggccagc gaaatggccg tcggtaaacc tgccgccagt tctttcgcaa
    60841 acgttgggcc agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt
    60901 cctgtaacag acgtccggtt tctgcttcca gccctttggt tgcccacacc agacgcgcat
    60961 caggacgcat cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga
    61021 cgacgagaat attacggctg gctgccagcg cagtggcgag atcgctttca agatggagcg
    61081 tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg
    61141 cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtga
    61201 tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt
    61261 tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat
    61321 gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac cgcgggatac
    61381 catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag
    61441 gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac
    61501 ttcacacagg aacgcggttt cttcgcccag ggaggccgtt acggtaacac gcagtaccac
    61561 ttcgtaaacg tcatctgcca gttgagtaga tgccgtatcc agatcaagtt taacttctgg
    61621 ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaaa tatccttggt
    61681 ataaatacgt tggatctgga aagtcatttc ggtgttgttt tgttctgaca tgtgtagaaa
    61741 acccttaagt gttgtcctta aaatactgcg caatgtaatg ccaacgttca cacgttattt
    61801 cagcagggga tccagtccac cacgtgcatc caatgcatac aagtcatcac agccaccaat
    61861 gtgctgtgcg tcaataaaaa tctgtggaac cgtggtgcga ccgctgcgtt tgatcatctc
    61921 ttcacgctta gcggcattgc catcgatcgg caactcctga aaactcacgc ccttgctgct
    61981 tagcagtgct tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt
    62041 ggccataaat tagctcctgt tttttaccct gaaatttcat gttgcagggc ggcagcaaaa
    62101 tggagtgtcc ccagaagctt atatcaataa gtcgctgggg gcctaattgc agctaacggc
    62161 ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca ccaaaggcaa gttttcgcca
    62221 gcccagccag cgacgccttc tttcagtacg aatacttgtg caaaaccagc tttcgtcagc
    62281 gcgtttgcag gctcctggca ctgcatgcca gaaccatcta ccacgataac cggtttgtct
    62341 ttgtgcttct caagctcacc aacattgttg gctttgattt cgctcggcaa caggttgata
    62401 gaacctgcga tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg
    62461 tcttctttgt tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc
    62521 aggctcttaa acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt
    62581 atgggatgac ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt
    62641 agtgattaaa aaaccaggaa aggagtatac ctgcgcagtg cggcaaatac agccagcgcg
    62701 ttaactggaa tgcaattttg cggggcgcta cgaaaaaaaa gcgtaaatct ggcatctgct
    62761 tgccgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    62821 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    62881 ctatggtact ggtgattctg gatggctatg gctatcgcga agaacagcag gataacgcca
    62941 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    63001 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    63061 gccacgttaa cctgggtgca ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    63121 aaatcaaaga tcgcactttc tttgctaatc cggtgttgac tggtgcggta gataaagcga
    63181 aaaacgcagg caaagcagta cacattatgg gtctgctctc cgcaggtggt gtacacagcc
    63241 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    63301 acctacacgc attccttgac ggtcgcgaca ctccgccgcg cagtgctgaa tcctcgctga
    63361 aaaaattcga agagaaattt gccgcgctgg gtaaaggccg cgtagcgtcc atcattggtc
    63421 gttactacgc gatggaccgc gataaccgtt gggatcgcgt tgaaaaagct tatgacctgc
    63481 tgactctggc gcaaggtgag ttccaggccg ataccgccgt tgccggtttg caggctgctt
    63541 atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc
    63601 cagatgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg
    63661 cacgtgaaat cactcgtgca ttcgtaaatg ccgattttga cggcttcgcg cgtaagaaag
    63721 tggttaacgt cgatttcgtg atgctgaccg aatacgctgc cgacatcaaa accgcagtgg
    63781 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    63841 ctcagctgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    63901 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    63961 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcaa
    64021 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    64081 acaccggggt gatggaagcg gcggttaaag cggttgaagc gctggatcac tgcgtggaag
    64141 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    64201 acgctgaaca gatgcgcgat ccggcaacgg gtcaggcaca cacagcacac accaacctgc
    64261 cagttccgct gatttacgtt ggtgataaga acgtgaaagc ggttgaaggc ggcaaacttt
    64321 ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    64381 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    64441 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    64501 tgagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    64561 aatccattca ggctgacatc gccgcgaaag agcgcgcggt acgccaaaag caacaacaac
    64621 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa gccacccgta
    64681 aactgcgcga aacgcaaaac acgcttaatc aactgaataa acagattgat gagatgaacg
    64741 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcgcaac
    64801 tggatgccgc gtttcgtcag ggtgaacata ccggtattca gctgattctc agcggtgaag
    64861 aaagccagcg tgggcagcgg ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    64921 aaaccattgc tcagttgaaa caaacgcgtg aagaagtcgc tatgcagcgt gccgaactgg
    64981 aagagaaaca aagcgagcaa caaacgcttt tatatgagca gcgcgcccaa caggcgaagc
    65041 tgacccaggc gttgagcgag cgtaaaaaga cgctggcagg gctggagtct tccatccagc
    65101 aaggtcagca acagttgagc gagctgcgcg ccaacgaatc ccgcctgcgt aacagcattg
    65161 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgtga agcgcgcgaa gcccaggcgg
    65221 ttcgcgaccg ccagaaagaa gcgacgcgca aaggcaccac ctacaagccg accgaaagcg
    65281 aaaaatcgct gatgtcccgt accggtggtc tgggcgcacc gcgcggtcag gcattctggc
    65341 cggttcgcgg gccaacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    65401 aagggatggt gattggtgcc tctgaaggta ctgaagttaa agcgattgcc gacggccggg
    65461 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag catggtaaag
    65521 gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc
    65581 gcgcgggcca gccaattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct
    65641 atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag
    65701 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    65761 ccgtaattgc tggcaaactt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg
    65821 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    65881 acgccagaga gatggcgacc aaagcacata acagcggtca cgaagtgttg attcatctac
    65941 cgatggcacc actgagtaaa cagccgctgg agaaaaatac gctacgcccg gagatgagca
    66001 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    66061 tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    66121 aggcgctgga gcgttacaat ctttactttc tcgacagcgt aaccatcggt aatacccagg
    66181 cgatgcgcgc cgcgcaaggc actggcgtga aggtgatcaa acggaaggtg ttcctcgacg
    66241 attcgcagaa cgaagcggac atccgtgtgc aattcaatcg cgcaattgac ctagcgcgtc
    66301 gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac
    66361 agctggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    66421 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    66481 cgttccgtgg cgtgaagctg tgcaaaccga agaagccgct ggaacctgtt tatgctaatc
    66541 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccagc
    66601 atcagtggca aggctggggc aaacagcccg aagcggcaaa acttaacgct agcgcaaatt
    66661 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    66721 gaaagaccac agtaacgcct ggtaacccac cttcacgctg cggacattgg taatcaggcg
    66781 cttatacata ccggaagtaa atatctcggc aatcatccgt tggcgggtaa gaatatccgg
    66841 ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc
    66901 cggataaatc gtaattttgt cagcataatt tcgatttaat ttctccagca ggcgggtaat
    66961 cttaatatag tgacgttgat aattaaggtt tttattccct tgtctatgca accgactcac
    67021 tgacgtatta tgcagataat atttatataa tgattgctcg gtatatcgcg cacgcagcgc
    67081 gttaaacata aattctgttg tccagacaat atcctgatga tgtaatc
//
LOCUS       DAOFSV010000008        66205 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.008, whole genome shotgun sequence.
ACCESSION   DAOFSV010000008 DAOFSV010000000
VERSION     DAOFSV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.008"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..679)
                     /locus_tag="QBH27_000591"
     CDS             complement(<1..679)
                     /locus_tag="QBH27_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDQ7012955.1"
                     /translation="MTIYFINWVADYELKMIQYLKKKHKIKNITTPKKYNWVNKKISK
                     LGMDNAWLGRLFIKHHLNAVKKDDIIIFNDSVINKSINKQILKNINCHKVLLLRNTVS
                     ENFIFDNANYFDIIYDFEHRFIGNEKIKAIEQFFPIGMDEIRNYSLSDKNNSQPICFF
                     LGRDKGRLQIINELADRLTTLGCKLDFNVVKDKTSSPTSKYLIEKQISYEENIRRTLN
                     ANIIVDIT"
     gene            complement(716..1513)
                     /gene="rfaP"
                     /locus_tag="QBH27_000592"
     CDS             complement(716..1513)
                     /gene="rfaP"
                     /locus_tag="QBH27_000592"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDQ7012956.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLRKILSFERNLLNMASVKAEHIKERTQRKGL"
     gene            complement(1506..2630)
                     /locus_tag="QBH27_000593"
     CDS             complement(1506..2630)
                     /locus_tag="QBH27_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDQ7012957.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2627..3685)
                     /gene="rfaQ"
                     /locus_tag="QBH27_000594"
     CDS             complement(2627..3685)
                     /gene="rfaQ"
                     /locus_tag="QBH27_000594"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDQ7012958.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPANWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4099..5376
                     /gene="waaA"
                     /locus_tag="QBH27_000595"
     CDS             4099..5376
                     /gene="waaA"
                     /locus_tag="QBH27_000595"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDQ7012959.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5384..5863
                     /gene="coaD"
                     /locus_tag="QBH27_000596"
     CDS             5384..5863
                     /gene="coaD"
                     /locus_tag="QBH27_000596"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDQ7012960.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5902..6711)
                     /gene="mutM"
                     /locus_tag="QBH27_000597"
     CDS             complement(5902..6711)
                     /gene="mutM"
                     /locus_tag="QBH27_000597"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDQ7012961.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6809..6976)
                     /gene="rpmG"
                     /locus_tag="QBH27_000598"
     CDS             complement(6809..6976)
                     /gene="rpmG"
                     /locus_tag="QBH27_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDQ7012962.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(6997..7233)
                     /gene="rpmB"
                     /locus_tag="QBH27_000599"
     CDS             complement(6997..7233)
                     /gene="rpmB"
                     /locus_tag="QBH27_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDQ7012963.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7450..8118)
                     /gene="radC"
                     /locus_tag="QBH27_000600"
     CDS             complement(7450..8118)
                     /gene="radC"
                     /locus_tag="QBH27_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDQ7012964.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8290..9510
                     /gene="coaBC"
                     /locus_tag="QBH27_000601"
     CDS             8290..9510
                     /gene="coaBC"
                     /locus_tag="QBH27_000601"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDQ7012965.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVCVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9488..9946
                     /gene="dut"
                     /locus_tag="QBH27_000602"
     CDS             9488..9946
                     /gene="dut"
                     /locus_tag="QBH27_000602"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDQ7012966.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            10053..10649
                     /gene="slmA"
                     /locus_tag="QBH27_000603"
     CDS             10053..10649
                     /gene="slmA"
                     /locus_tag="QBH27_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDQ7012967.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10686..11327)
                     /gene="pyrE"
                     /locus_tag="QBH27_000604"
     CDS             complement(10686..11327)
                     /gene="pyrE"
                     /locus_tag="QBH27_000604"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDQ7012968.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(11393..12109)
                     /gene="rph"
                     /locus_tag="QBH27_000605"
     CDS             complement(11393..12109)
                     /gene="rph"
                     /locus_tag="QBH27_000605"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDQ7012969.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12236..13099
                     /locus_tag="QBH27_000606"
     CDS             12236..13099
                     /locus_tag="QBH27_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDQ7012970.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            13351..13968
                     /locus_tag="QBH27_000607"
     CDS             13351..13968
                     /locus_tag="QBH27_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDQ7012971.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(13965..15647)
                     /gene="ligB"
                     /locus_tag="QBH27_000608"
     CDS             complement(13965..15647)
                     /gene="ligB"
                     /locus_tag="QBH27_000608"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDQ7012972.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMIPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            15905..16528
                     /gene="gmk"
                     /locus_tag="QBH27_000609"
     CDS             15905..16528
                     /gene="gmk"
                     /locus_tag="QBH27_000609"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDQ7012973.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16583..16858
                     /gene="rpoZ"
                     /locus_tag="QBH27_000610"
     CDS             16583..16858
                     /gene="rpoZ"
                     /locus_tag="QBH27_000610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDQ7012974.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            16877..18985
                     /gene="spoT"
                     /locus_tag="QBH27_000611"
     CDS             16877..18985
                     /gene="spoT"
                     /locus_tag="QBH27_000611"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDQ7012975.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            18992..19681
                     /gene="trmH"
                     /locus_tag="QBH27_000612"
     CDS             18992..19681
                     /gene="trmH"
                     /locus_tag="QBH27_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDQ7012976.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            19687..21768
                     /gene="recG"
                     /locus_tag="QBH27_000613"
     CDS             19687..21768
                     /gene="recG"
                     /locus_tag="QBH27_000613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDQ7012977.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(21802..23007)
                     /gene="gltS"
                     /locus_tag="QBH27_000614"
     CDS             complement(21802..23007)
                     /gene="gltS"
                     /locus_tag="QBH27_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDQ7012978.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            23287..24678
                     /gene="xanP"
                     /locus_tag="QBH27_000615"
     CDS             23287..24678
                     /gene="xanP"
                     /locus_tag="QBH27_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDQ7012979.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            24799..26508
                     /locus_tag="QBH27_000616"
     CDS             24799..26508
                     /locus_tag="QBH27_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDQ7012980.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLKNVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFAPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(26552..27232)
                     /locus_tag="QBH27_000617"
     CDS             complement(26552..27232)
                     /locus_tag="QBH27_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDQ7012981.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(27213..28145)
                     /locus_tag="QBH27_000618"
     CDS             complement(27213..28145)
                     /locus_tag="QBH27_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDQ7012982.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(28193..29053)
                     /locus_tag="QBH27_000619"
     CDS             complement(28193..29053)
                     /locus_tag="QBH27_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDQ7012983.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(29134..29985)
                     /locus_tag="QBH27_000620"
     CDS             complement(29134..29985)
                     /locus_tag="QBH27_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDQ7012984.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(29997..31088)
                     /locus_tag="QBH27_000621"
     CDS             complement(29997..31088)
                     /locus_tag="QBH27_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDQ7012985.1"
                     /translation="MKENKITVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(31113..31427)
                     /locus_tag="QBH27_000622"
     CDS             complement(31113..31427)
                     /locus_tag="QBH27_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDQ7012986.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(31445..31915)
                     /locus_tag="QBH27_000623"
     CDS             complement(31445..31915)
                     /locus_tag="QBH27_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ7012987.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(31942..33495)
                     /locus_tag="QBH27_000624"
     CDS             complement(31942..33495)
                     /locus_tag="QBH27_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDQ7012988.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVDYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(33790..36108)
                     /gene="yicI"
                     /locus_tag="QBH27_000625"
     CDS             complement(33790..36108)
                     /gene="yicI"
                     /locus_tag="QBH27_000625"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDQ7012989.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(36118..37500)
                     /locus_tag="QBH27_000626"
     CDS             complement(36118..37500)
                     /locus_tag="QBH27_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDQ7012990.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            37793..37887
                     /locus_tag="QBH27_000627"
     tRNA            37793..37887
                     /locus_tag="QBH27_000627"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37827..37829,aa:Sec,seq:tca)
     gene            37940..38977
                     /locus_tag="QBH27_000628"
     CDS             37940..38977
                     /locus_tag="QBH27_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDQ7012991.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            39547..40044
                     /locus_tag="QBH27_000629"
     CDS             39547..40044
                     /locus_tag="QBH27_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012992.1"
                     /translation="MGHGTCKAANSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLFQNNNALYLFENDNKIELRMGHDSFFSIETIDKINKENIKGIKLKN
                     ETTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDTAPCFIIGIPDEYTKAIE
                     LLNIK"
     gene            40222..41145
                     /locus_tag="QBH27_000630"
     CDS             40222..41145
                     /locus_tag="QBH27_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ7012993.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(41149..41967)
                     /gene="nlpA"
                     /locus_tag="QBH27_000631"
     CDS             complement(41149..41967)
                     /gene="nlpA"
                     /locus_tag="QBH27_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDQ7012994.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            42189..42482
                     /locus_tag="QBH27_000632"
     CDS             42189..42482
                     /locus_tag="QBH27_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7012995.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(42523..43713)
                     /gene="nepI"
                     /locus_tag="QBH27_000633"
     CDS             complement(42523..43713)
                     /gene="nepI"
                     /locus_tag="QBH27_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDQ7012996.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            43947..44300
                     /locus_tag="QBH27_000634"
     CDS             43947..44300
                     /locus_tag="QBH27_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ7012997.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHRNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            44284..44607
                     /locus_tag="QBH27_000635"
     CDS             44284..44607
                     /locus_tag="QBH27_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDQ7012998.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSRVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(44723..45175)
                     /locus_tag="QBH27_000636"
     CDS             complement(44723..45175)
                     /locus_tag="QBH27_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDQ7012999.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(45228..46562)
                     /gene="adeQ"
                     /locus_tag="QBH27_000637"
                     /gene_synonym="yicO"
     CDS             complement(45228..46562)
                     /gene="adeQ"
                     /locus_tag="QBH27_000637"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDQ7013000.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSISSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            46737..48503
                     /gene="adeD"
                     /locus_tag="QBH27_000638"
     CDS             46737..48503
                     /gene="adeD"
                     /locus_tag="QBH27_000638"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDQ7013001.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(48550..49941)
                     /gene="uhpT"
                     /locus_tag="QBH27_000639"
     CDS             complement(48550..49941)
                     /gene="uhpT"
                     /locus_tag="QBH27_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDQ7013002.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(50079..51398)
                     /gene="uhpC"
                     /locus_tag="QBH27_000640"
     CDS             complement(50079..51398)
                     /gene="uhpC"
                     /locus_tag="QBH27_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDQ7013003.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNSAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(51408..52910)
                     /gene="uhpB"
                     /locus_tag="QBH27_000641"
     CDS             complement(51408..52910)
                     /gene="uhpB"
                     /locus_tag="QBH27_000641"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDQ7013004.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(52910..53500)
                     /gene="uhpA"
                     /locus_tag="QBH27_000642"
     CDS             complement(52910..53500)
                     /gene="uhpA"
                     /locus_tag="QBH27_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDQ7013005.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            53984..54844
                     /locus_tag="QBH27_000643"
                     /pseudo
     CDS             53984..54844
                     /locus_tag="QBH27_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525915.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54910..55200)
                     /gene="ilvN"
                     /locus_tag="QBH27_000644"
     CDS             complement(54910..55200)
                     /gene="ilvN"
                     /locus_tag="QBH27_000644"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDQ7013006.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(55204..56892)
                     /gene="ilvB"
                     /locus_tag="QBH27_000645"
     CDS             complement(55204..56892)
                     /gene="ilvB"
                     /locus_tag="QBH27_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDQ7013007.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFSIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKHPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(56998..57096)
                     /gene="ivbL"
                     /locus_tag="QBH27_000646"
     CDS             complement(56998..57096)
                     /gene="ivbL"
                     /locus_tag="QBH27_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDQ7013008.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            57497..58681
                     /gene="emrD"
                     /locus_tag="QBH27_000647"
     CDS             57497..58681
                     /gene="emrD"
                     /locus_tag="QBH27_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDQ7013009.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVSTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(58689..59186)
                     /locus_tag="QBH27_000648"
     CDS             complement(58689..59186)
                     /locus_tag="QBH27_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDQ7013010.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(59183..59545)
                     /locus_tag="QBH27_000649"
     CDS             complement(59183..59545)
                     /locus_tag="QBH27_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDQ7013011.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(59535..59882)
                     /locus_tag="QBH27_000650"
     CDS             complement(59535..59882)
                     /locus_tag="QBH27_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDQ7013012.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(59942..61436)
                     /locus_tag="QBH27_000651"
                     /pseudo
     CDS             complement(59942..61436)
                     /locus_tag="QBH27_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(61433..63148)
                     /locus_tag="QBH27_000652"
     CDS             complement(61433..63148)
                     /locus_tag="QBH27_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDQ7013013.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKTAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            63315..64181
                     /locus_tag="QBH27_000653"
     CDS             63315..64181
                     /locus_tag="QBH27_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ7013014.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(64271..65932)
                     /locus_tag="QBH27_000654"
     CDS             complement(64271..65932)
                     /locus_tag="QBH27_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDQ7013015.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(66130..>66205)
                     /locus_tag="QBH27_000655"
     CDS             complement(66130..>66205)
                     /locus_tag="QBH27_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="16 kDa heat shock protein B; associates with
                     aggregated proteins, together with ibpA, to stabilize and
                     protect them from irreversible denaturation and extensive
                     proteolysis during heat shock and oxidative stress;
                     ATP-independent; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat-shock protein"
                     /protein_id="HDQ7013016.1"
                     /translation="LIRNEPEPIAAQRIAISERPALNS"
BASE COUNT        17043 a        17410 c        15948 g        15804 t
ORIGIN      
        1 tagttatatc aacaattata ttagcattta atgttcttct aatattctct tcatacgata
       61 tttgtttttc aatcaaatac tttgaagtcg gagatgatgt tttatctttg acaacattaa
      121 aatctaactt acatcctagt gttgttaatc tatcggctaa ttcattaatt atttgaagac
      181 gtcctttatc acgaccaaga aaaaaacata ttggctgact gttatttttg tcagataaac
      241 tataatttct aatttcatcc attcctatag gaaaaaattg ttctattgcc ttgatctttt
      301 cattccctat aaatctatgc tcaaaatcat agataatatc aaaataatta gcgttatcaa
      361 aaataaaatt ttcactgaca gtatttctta gaagtaatac tttatgacaa ttaatatttt
      421 ttaatatttg cttgttaata cttttattaa tgacactgtc attaaatata atgatatcat
      481 ccttttttac agcatttagg tgatgtttga taaataatcg tcccaaccat gcattatcca
      541 tccctaattt cgaaattttc ttattaaccc agttgtattt ttttggtgtt gttatatttt
      601 ttatcttgtg cttttttttc aagtactgga tcatttttaa ttcataatct gcaacccagt
      661 ttataaaata aatagtcata atgaagttag ttccagtaca tactgattaa tatttttata
      721 atcctttgcg ttgtgttcgc tctttaatat gctcggcctt aacacttgcc atgttcaata
      781 aattccgttc aaaagataat atcttacgta aaggcatccc aaaataaacc ttcataaatc
      841 gccagatatc tctctgcgtc agcccaatat tcattgatga aaaatacaaa ccaatcaaat
      901 ctttatcccg ccaacgacgc ggtacttttg cacgtatctg cgcccggtgc aggtcaatta
      961 ctgaaatttt taactcctct tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga
     1021 tataacagtc acggtggtta atgcccgcag catgcatatc gcgcaccatc gtcgcgacac
     1081 gcttaataag catacgcttc acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt
     1141 aatcttccag actgatggtt ggtgtcagat cttcggtaat aataaacgag gttctggtca
     1201 gcggattaat gcctttttcg ccgaatgcca ccccatacat agtatcaacg ccgacatccc
     1261 gcagtcgatg aatcgcattc cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga
     1321 gtaaattttt gattatctct ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt
     1381 tgcccgccat ttcaaagcgc agagtgcggc gagtttccag ttcacgaaat acctcaccct
     1441 gcaaggtttt aacttcctca aaaggatctt taccgcgcca taacgtggca aacggctctt
     1501 taagttcaac catccagacc acccgttatg atgtccgccg ctttctctgg cagactgtat
     1561 aaatcttgtg tatcagcata atgtcgcgca ttttccgccc aggcctggcg caatgaagat
     1621 tgcgttaacg ctttgcgtaa aatctcattc aatgtttcct ggcggaatgg ctcagcaata
     1681 gcctcgccgc aattggcgtc aacaatataa tgcgcatagc cacaaacggc tgttgttagt
     1741 accggtaatc ctgcagttat cgcttccagc agtacaattc ccgcggcttc ctggtaggca
     1801 ggatgaagta ataaatccgc cgccgccatt aattccgaga catcgttgcg ccccgagaag
     1861 aagtgaacat tactgcgcac gccgcgtttt tctgccagcg cctcaaattt tcgcggttta
     1921 tcctgcccaa caacatatag caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt
     1981 gcttcaatgg aacgatcgac acctttacgc gtgaagtctg aaccaacctg caacaataaa
     2041 tattgttgtt cggttattcc attcttctta cggaagattt cacggctatt ggctggctgc
     2101 tggctatatt tacgatcagg ataaatcccc ggtggcagaa tatgaaaacg ttccgcttca
     2161 gtctgataat gtttctggaa atcggcgatt tgcttatctg tcagcatcag cagctgtgtc
     2221 ggcttgccct gttcgaaggt tgcccgctca aaggcagcat aatggcgata acgtgacgtc
     2281 aggcgataga aaaagccttt ctcctgcgct actttctcgg cataacaaac atcagcggca
     2341 taataaacgt ccagccccgg cattttatta aatccaacaa ctctatcgac ggggtgttca
     2401 cgtaaatgtt tttgcaccca ggcaaaatat tccgcattgc gcccgtgatt ggtatgcgat
     2461 ttaaccggca ctttgatcag ttcaaataca tcagggcatt cgccttccca cgactgggta
     2521 taaacccgaa catgatggcc tcgggcggcg actgtctgag caatacgcat aaaatcgcgc
     2581 tgcaaaccgc caaagggaaa atatttatat aaacaaaaag caacgatcat aacgacgtac
     2641 ctgtcgtgga ggagggaagt aatttatcga cagctgcaat gacatctgcc gccggaataa
     2701 ccgaaagata catctcattt cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc
     2761 ccgcccagaa ttgaatcatg ttatttgacc agggacgcca gaaaatatgg tccgtcgcac
     2821 caaacagcga tatcagcggc gtattaactg cagcggcaat atgcgccggt gcggaatcaa
     2881 cgccaataaa cagctgcgca tgatcgatta acgcaccaag ttccgggaag gtcacctttc
     2941 cggccagcgc cgttactggt ggcgtctggc atccctgcgc aatttcattg acgcaggcca
     3001 gatcgtcttt atctgggccg gacgtcagaa cgacttcata accacgagca tgtaaggcat
     3061 caatcacagc ggaaaacttg gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt
     3121 ggataaccac ataattttgt ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc
     3181 agtttgcagg cgggtaactc atggttgtct gcttcaccaa cgattcaagt cccaatggcg
     3241 tcagcacgga taagttactt tccaccacat ttccaccctg caacggcacc aaatgagtga
     3301 aactattacg ccaaaaagca gactgccgat gatgataatc ctgggaaatt ttcacacggg
     3361 catttaataa gcgaaccagt atagcaacca tccattgatc ggtaagatta acgataaggt
     3421 catacttatt ggcacgtaat accttgatga gatgaaaaaa gttggcaatt ttttctgagg
     3481 cttttgcttt tttatttttt atgccgtaga gcgcgttaat ctctggattt tcagacagga
     3541 tcgggatggt gtcctgataa agcagcacat cgatttttgc gtcagggtaa ttttttttca
     3601 gcgaactaat gacgggagta gttaataaca tatccccatg aaaacgcatc ttaatgagca
     3661 aaattcttcg aaatggctta tccacaagta actctttttg ttgagattat ccgattaagg
     3721 tgaagcagaa aaagcacgct accgccccag gctcaacagc tacctgaata ctgataccgc
     3781 actgatattg acgcgctagt gtatcacttc tttcgcggca atccgacccg aatgattttt
     3841 aagcacattg ttgattttca ataaagaaat caatcaaata aaatcattag caaccatatt
     3901 ctttttactg ttgctctgcg attccttcct gcacgtactg cagaaatcaa tgaatcgcat
     3961 aaaaatacaa aaaaagtata gatgttcagc aagaattggc gggtaaattg ctatttaagt
     4021 caaaaacggg aaaagtaatg gtaaagccac agctaaatac atagaatccc cagcacatcc
     4081 ataagtcagc tatttactat gctcgaattg ctttacaccg cccttctcta ccttattcag
     4141 ccactgatct ggatacggct ctgggtgcgc ggacgtaagg ctccggccta tcgaaaacgc
     4201 tggggtgaac gttacggttt ttaccgccat ccgctaaaac caggcggcat tatgctgcac
     4261 tccgtctccg tcggtgaaac tctggcggcg atcccgttgg tgcgcgcact gcgtcatcgt
     4321 tatcctgatt taccgattac cgtaacaacc atgactccaa ccggttcgga gcgcgtacaa
     4381 tcggctttcg ggaaggatgt tcagcacgtt tatctgccgt atgacctgcc cgatgcactc
     4441 aaccgtttcc tgaataaagt cgaccctaaa ctggtgttga ttatggaaac cgaactatgg
     4501 cctaacctga ttgcggcgct acataaacgt aaaattccgc tggtgatcgc taacgcgcga
     4561 ctctctgccc gctcggccgc aggttatgcc aaactgggta aattcgtccg ccgcttgctg
     4621 cgtcgtatta cgctgattgc cgcccaaaat gaagaagatg gtgcacgttt tgtggcgctg
     4681 ggcgcaaaaa ataatcaggt gaccgttacc ggtagcctga aattcgatat ttctgtaacg
     4741 ccgcagttgg ctgctaaagc cgtgacgctg cgccgccagt gggcaccaca ccgcccggta
     4801 tggattgcca ccagcactca tgaaggcgaa gagagtgtgg tgatcgccgc acatcaggca
     4861 ttgttacagc aattcccgaa tttattgctc atcctggtac cccgtcatcc agaacgcttc
     4921 ccggatgcga ttaaccttgt ccgccaggct ggactaagct atatcacacg atcttcaggg
     4981 gaagtcccct ccaccagcac gcaggttgtg gttggcgata cgatgggcga gttgatgtta
     5041 ctgtacggca ttgccgatct cgcctttgtt ggcggttcac tggttgaacg tggtgggcat
     5101 aatccgctgg aagcggccgc acacgctatt ccggtattga tggggccgca tacgtttaac
     5161 tttaaagaca tttgcgcgcg gctggagcag gcaagcgggc tgattaccgt taccgatgcc
     5221 actacgcttg caaaagaggt ttcctcttta ctcaccgacg ccgattaccg tagtttctat
     5281 ggccgtcatg ccgttgaagt gctgtatcaa aaccagggcg cactacagcg tctgcttcaa
     5341 ctgctggaac cttacctgcc accgaaaacg cattgaggtt gttatgcaaa aacgggcgat
     5401 ttatccgggt actttcgatc ccattaccaa tggtcatatc gatatcgtga cgcgcgcctc
     5461 gcaaatgttc gatcacgtta ttctggcgat tgccgctagt cccagtaaaa aaccgatgtt
     5521 taccctggaa gagcgtgtgg agctggcaca gcaggcaacc gcgcatctgg agaacgtgga
     5581 agtggtcggg tttagtgatt tgatggcgaa cttcgcccgt aatcaacacg ctacggtgct
     5641 gattcgtggc ctgcgtgcgg tggcggattt tgaatatgaa atgcagctgg cgcatatgaa
     5701 tcgccactta atgccggagc tggaaagtgt gtttctgatg ccgtcgaaag agtggtcgtt
     5761 tatctcttca tcgttggtga aagaggtggc gcgccatcag ggcgatgtca cccatttcct
     5821 gccggagaat gtccatcagg cgctgatggc gaagttagcg tagagtttat gccggatggt
     5881 atgccatccg gcgcgcatca attacttctg gcactgccga caataaaacg ttgcccgctg
     5941 cgcatgttta gtcgccacaa tcggcgtacc gcacacccga cacggctcac cttttcgccc
     6001 gtaaacctgc aattcctgag cgaaatagcc cggtttacca tcactttgca gaaaatcttt
     6061 cagcgttgta ccgccctgct caatcgaacg cagcaacacc gctttaatca cccgagctaa
     6121 caattcacac tccgccagcg acagtgatga cgccagccga tccggatgga tccccgccgc
     6181 aaacagcgat tcgctggcat aaatattccc tacgcctacc accagcttgt tatccatcag
     6241 ccacggttta atcgccgttt ttttcttcgc gcacttctga tgcaggtact caccgttgaa
     6301 atcgtcacta agcggctccg gtccaagatg ggccagcacg ttgtgccctt ccagctcttt
     6361 ggtccatagc caggcaccaa agcggcgcgg atcggtgtag cgcagcactt tgccgttgct
     6421 catcaccaaa tccacatggt catgcttttc agggggaagt tcttctggaa ggatgcgcag
     6481 gctgccagac atccccaaat gaatgataat ccagccctca ggcagctcca gcagcagata
     6541 tttagcccgg cgctgcacgc taagcactgg ttggtcgctt aaacggtaga tctcttctga
     6601 aaccggccag cgcaagcgtc cgttgcgcac caccgcatga agaatggttg caccaacgag
     6661 atgcggttct atgccgcgac ggctggtttc aacttcgggt aattcaggca tagcatctcc
     6721 aggaatgaac agatgcagtc aatatggggg caagcagata acaaaaaacc ccgccggggc
     6781 gaggtttttt gttacattaa agcggaaatt atttgatttt cgcttctttg tagatcacgt
     6841 gctggcgaac aactggatcg aattttttca gttccagttt ttccggctta gtacgtttgt
     6901 tcttcgtagt ggtatagaag tgaccagtac cagcagaaga aaccagcttg attttctcac
     6961 gaataccttt agccatgatt tatttcctct aagtacttag tacttttcgc cacgggcacg
     7021 cagttcagcc agaactgtat cgatgccttt tttatcgatt acacgcatac ctttagcaga
     7081 tacgcgcagg gtgacaaaac gcttctcgct ctcaacccag aaacggtgag agtgcaggtt
     7141 cggcaggaaa cggcgtttag tcgcgttcag tgcgtgggaa cggttgttac cggtcaccgg
     7201 acgcttgcca gtaacttggc agactcggga catgtctatt ctccaaaaat caaattagct
     7261 cgagcttcgt atggggtatt ggcgcctcgt caggctttac agcccggtca tcgcagttct
     7321 atgtgaactc tcgattgcca ggcccaaatg ccaaacccga gattctcaaa ggtggcgtag
     7381 tatacgctga ctcagcgatg tgctcaagtc ccgaacagac aaagatcccg aaggatcgcg
     7441 catagcgggt taaatccagc cgcgttcggc aaaagaaaca tactctccac gcccaatcac
     7501 gatatggtcg agcacgcgta aatccatgaa ctgacaactc tttattatcc gttcagtaat
     7561 gagtttatcc gctttactgg gttcagcaca acccgaaggg tgattatgcg caaggatcag
     7621 cgccgaggcg tttattttta tcgcttcgcg gataatttcc cgaggatgga cttcaacatg
     7681 gtttagcgtg ccggaaaaaa gacggctatg cgttataacc ctgtgttggg agtcgagaaa
     7741 gatcaccata aagatctccc gctcctcgcc cgtgagctgg ctttgtaaaa attccctcgt
     7801 catctccggg ctgagtaaag ggctttcttc acgcatccgt acgttgtagt aacgccgcgc
     7861 cagttcggca atccccttta actgggcgaa tttcgccacg ccaattccat gaacgccact
     7921 aaattgctca tattcagagg ttaacaagcc ataaagagag ccgaaattct ccagcatctc
     7981 ttttgccagg gttaatacat ctttaccgcg cgttccggta cgcagaaata gcgccagcag
     8041 ctcgacatcc gttaaggcgc taataccaaa cttcagcatt ttttcgcgcg gcatcaacag
     8101 ctgtacattg tttttcacct tcacctcctt tgtggtgctc gcatcctgtc atagccgttc
     8161 ctttaaatcg acggccagtt ttcaatcctg gaaagcgcct cgcaaagtga atctcaggtg
     8221 aaggcacgac aacaaaagta ttgtgataaa atcgccaact tctggtgtca cccaacagga
     8281 aaaatcatca tgagcctggc cggtaaaaaa atcgttctcg gcgttagcgg cggtattgct
     8341 gcctataaaa cccctgaact ggtgcgtcgt ttgcgcgatc gcggggccga tgtctgcgta
     8401 gccatgaccg aagcggcaaa agcctttatc accccactta gcttgcaggc ggtttctggt
     8461 tatcccgttt ccgacagtct gctggacccg gcagccgaag ccgctatggg ccatattgag
     8521 ctgggaaaat gggctgattt agtgattctc gcccctgcca cggcagattt aattgcccgt
     8581 gttgcagccg gaatggcgaa tgacctggta tcgacgattt gtctggctac acctgcgcct
     8641 gtagccgtgc tccccgccat gaaccagcag atgtaccgtg ccgccgccac ccagcataat
     8701 ttagaggtgc ttgcctcacg tggtttgctc atctgggggc cagacagcgg cagtcaggct
     8761 tgtggtgata tcggcccagg acgaatgctc gatccgttaa ctattgtgga tatggcggta
     8821 gcgcattttt cgcccgtcaa cgacctgaaa catctgaaca ttatgattac cgccggtccg
     8881 acgcgtgaac cgctcgatcc cgtgcgttat atctctaatc acagctccgg caagatgggt
     8941 tttgctatcg ccgccgccgc agcccgtcgt ggcgcgaacg tcacgctggt atcaggtccg
     9001 gtttcactac cgacgccacc gtttgttaac cgtgttgatg tgatgaccgc gctggaaatg
     9061 gaagccgccg tgaatgcttc agtacagcag caaaatattt ttatcggttg cgccgccgtg
     9121 gcggattatc gcgcagctac cgtggcccca gaaaaaatca aaaagcaggc cacgcagggt
     9181 gatgaattaa caataaaaat ggttaaaaac cccgatatcg tcgcaggcgt tgccgcacta
     9241 aaagaccatc gaccttacgt cgttggattt gccgccgaaa caaataatgt ggaagaatac
     9301 gcccggcaaa aacgtatccg taaaaacctt gatctgatct gcgcgaacga tgtttcccag
     9361 ccaactcaag gatttaacag cgacaacaac gcattacacc ttttctggca ggacggagat
     9421 aaagtcttac cgcttgagcg caaagagctc cttggccaat tattactcga cgagatcgtg
     9481 acccgttatg atgaaaaaaa tcgacgttaa gattctggac ccgcgcgttg ggaaggaatt
     9541 tccgctcccg acttatgcca cctctggctc tgccggactt gacctgcgtg cctgtctcga
     9601 cgacgccgta gaactggctc cgggtgacac tacgctggtt ccgaccgggc tggcgattca
     9661 tattgccgat ccttcactgg cggcaatgat gctaccgcgc tccggattgg gacataagca
     9721 cggtatcgtg cttggtaacc tggtaggatt gatcgattct gactatcagg gccagttgat
     9781 gatttccgtg tggaaccgtg gtcaggacag cttcaccatt caacctggcg aacgcatcgc
     9841 ccagatgatt tttgttccgg tagtacaggc tgaatttaat ctggtggaag atttcgacgc
     9901 caccgaccgc ggtgaaggcg gctttggtca ctctggtcgt cagtaacaca tacgcatccg
     9961 aataacgtca taacatagcc gcaaacattt cgtttgcggt catagcgtgg gtgccgcctg
    10021 gcaagtgctt attttcaggg gtattttgta acatggcaga aaaacaaact gcgaaaagga
    10081 accgtcgcga ggaaatactt cagtctctgg cgctgatgct ggaatccagc gatggaagcc
    10141 aacgtatcac gacggcaaaa ctggccgcct ctgtcggcgt ttccgaagcg gcactgtatc
    10201 gccacttccc cagtaagacc cgcatgttcg atagcctgat tgagtttatc gaagatagcc
    10261 tgattactcg tatcaacctg attctgaaag atgagaaaga caccacagcg cgcctgcgtc
    10321 tgatcgtgtt gttgctcctc ggttttggtg agcgtaatcc tggcctgact cgcatcctca
    10381 ctggtcatgc gctaatgttc gaacaggatc gtctgcaagg gcgcatcaac cagctgttcg
    10441 agcgtattga agcgcagttg cgtcaggtat tgcgtgaaaa gagaatgcgt gagggtgaag
    10501 gttacaccac cgatgaaacc ctgctggcaa gccagttgct ggcattttgt gaaggtatgc
    10561 tgtcacgatt tgtccgcagc gagtttaaat accgcccgac ggatgatttt gacgcccgct
    10621 ggccgctaat tgccgcacag ttgcagtaat atgacgccgg atgacttttc atccggcgag
    10681 tttcgttaaa cgccaaactc ttcgcgatag gccttaaccg ccgccagatg ttccgccatt
    10741 tctggcttct cttccaggta agcaatcagg tctttcaggg tgatgataga gatcaccttg
    10801 cagttgtaat cacgctcaac ttcctgaatc gccgaaattt cgccgcgccc gcgttcctga
    10861 cgatcaagcg aaatcaacac gcctgcaagc gtcgcgccat tggcctgaat aatttccatc
    10921 gactcgcgaa tcgccgttcc ggcggtgatc acatcatcta ccagcattac gcgtccttgt
    10981 aacgcgctac caaccagatt accgccttca ccgtggtctt ttgcctcttt gcggttaaag
    11041 cagtacggta ggtcaaggtc atgatgctcc gccagcgcca cggcggtagt ggtggcaatc
    11101 gggatccctt tgtaagcagg gccaaacagc agatcgaact caatgccgga atccaccaat
    11161 gcttcagcgt aaaaacggcc taacagtgcc agatcgcgcc cggtattaaa cagcccggcg
    11221 ttgaagaaat aggggctttt gcgcccggat ttcagcgtaa actcgccaaa ctttaacacc
    11281 tgcttgctaa gcgcaaattc aataaactgg cgctgatatg gtttcatgcc ttcgctcctc
    11341 atcttacttt tctacagaca aaaaaaaggc gactcatcag tcgccttaaa aatcagtttg
    11401 ccagcgccgc cttctgcgtc gctacaatgg attcgattcc ccctcgggcc agcgccaaca
    11461 aggtgagtaa ctcttcgtgg gtgaacggtt cgccttctgc cgtcccctgc acttcaatga
    11521 tacgcccgtc ttcggtcatc actacgttca tgtcggtctc tgcggcagag tcttcaacat
    11581 attccagatc gcaaaccgct tcaccgttca caatcccgac agagaccgcg gctaccatcc
    11641 ctttcattgg attagttttc agcttgccgt tttccaccag cttctgtagc gcatctgcca
    11701 gcgccacgca ggcacccgta atcgaagcgg tacgcgtgcc accatcagcc tgaagcacgt
    11761 cacagtccag cgtaatggtg aactcaccca gtgctttcaa atctactgcc gcgcgaagag
    11821 cacgggcgat tagacgctgg atttccattg tgcgtccacc ctgcttacct ttcgccgctt
    11881 cacgcgcgtt acgggtatgg gtagaacgcg gcagcatacc gtactctgcg gtgatccagc
    11941 cctggccctg acctttcagg aagcgcggca cgccttcttc aatagaggcg gtacacaata
    12001 ctttggtatc gccaaattcg accagcaccg agccttctgc atgttttgta tagttacgag
    12061 tcagggtaac gggacgcacc tgattattgc tacggcctgc tggacgcata ttgaaatctc
    12121 cggcttgaaa caaatgtggc tgcgcattat acggacttcc ggcggttatt cctatcctga
    12181 caaggcatcg atggctataa tccttccacc tctcctttta taaacaggaa cgtctatgat
    12241 ccgcagtatg accgcctacg cccggcgtga aatcaagggt gaatggggga gcgcaacctg
    12301 ggaaatgcgc tcggtaaacc agcgttatct ggaaacttac tttcgtctgc cggagcagtt
    12361 ccgtagcctt gaacctgtcg ttcgcgagcg tattcgttct cgcctgacgc gcggtaaagt
    12421 ggaatgtact ctgcgctatg agccagatgt tagcgcgcaa ggtgagctga tcctcaacga
    12481 aaaactggct aaacagctgg taactgccgc gaactgggta aaaatgcaga gcgacgaagg
    12541 agaaatcaac ccggttgata ttctacgctg gccgggcgtg atggcagccc aggagcaaga
    12601 tctcgacgcg attgcagctg aaattctcgc ggcgttggat ggcaccctgg atgactttat
    12661 catcgcgcgc gaaaccgaag gtcaggcgct gaaagtgttg attgagcagc gtctggaagg
    12721 cgtcaccgcc gaagtggtca aagtccgcgc ccatatgccg gaaatcctgc aatggcagcg
    12781 tgagcgtctg gtagcgaagc tggaagatgc tcaggtgcaa ctggaaaaca accgtctgga
    12841 gcaggagttg gttctgctgg cacaacgaat tgacgttgcc gaagaactgg accgcctcga
    12901 agcgcatgtc aaagagacct acaacattct gaagaaaaaa gaagccgttg gtcgtcgtct
    12961 ggactttatg atgcaggagt tcaaccgcga gtcgaacact cttgcgtcga aatctatcaa
    13021 cgccgaagtg acaaactccg ccatcgagct gaaagtgttg attgaacaga tgcgcgagca
    13081 gattcagaat atcgagtaag cgacatatct ccgtaggccg ttgttaatta cagcggcgct
    13141 acgcatatag tttcgtcctc atatttattt actccatact ttttctaatg cattttactt
    13201 atcacaaaat cagaaaaatg cattcttact acagaaaata gcaatttcac ataactacaa
    13261 attatcaatc tattctctcc ttaaaaaatc tcaatcgtga caatgcgcac aaatcgctac
    13321 ccttccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    13381 attactgccg aagccatgac gggggcgctg gcggccggac gacggcgcat ggatacattt
    13441 ggcgtaatta ttattgcgac cgccacggca attggcggag ggtcagtgcg cgatattctg
    13501 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    13561 gctgcagtac tcaccactat agttgctcct gtaatgcctt atctgcgcaa agtgtttctg
    13621 gtgctcgatg cgctcggact ggtggtattt tctatcatcg gcgcacaggt tgctctggat
    13681 atggggcacg gcccgattat tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc
    13741 gttttgcgcg atatgttctg taaacgcatc ccactggtat tccagaaaga gttgtatgcg
    13801 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    13861 gatgtggtga ttatctccac cctggtattc ggcttttttg cccgtttact ggcgctacgt
    13921 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    13981 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    14041 tcaataacct gtctggcgcg ccccgatcca gtccccggca gctgctgcca gaactgttcc
    14101 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgagtt
    14161 agcggtattc ccattgccat cacccagcga gtaaaaggct gctgacgagc cagattaaac
    14221 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    14281 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagggcgcgc
    14341 cagccggcct caccaatgcc atcaagcccc agaacctgtt ttgaccccag ccagactaag
    14401 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgagttaaag
    14461 cggttttctg gtggtgtcgg ttttgtacgt tctgctccgc gccagaccac atcatcaatg
    14521 cgaggaatcc cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    14581 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    14641 acaggtacaa gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    14701 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    14761 tggcccggta gccaatgacg ggattcgggt tctttcgccg ctcgcacaac cacgccatcg
    14821 gtgacgaagg gtaattttgc tttccaccac tcattgcgta cgtgcgcaac ctcatcagca
    14881 tttttaaccg cgcgggtata cgtctgcgtt aaagtaaaac ctgcggtagc cagatccttt
    14941 aaacgatcgg tcattaaatg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    15001 tttagcgtat cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    15061 attcccccca tttgttgttg gatatgcccc ttacgcttga gaaatatttc cccctgaagc
    15121 gtgctgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gcgaactttc
    15181 tgcgtccagt cctcgccttt caggccattg ccgcgactga ttgctttgtt cagtttcccg
    15241 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    15301 cgttcgcgca tccaaagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    15361 gcaaccggat gcataaccgc accgtttaaa ggcggtatca tcacatcgcg ggtctcgttc
    15421 ccaaaacagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    15481 acctcacttt tcccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    15541 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacaccgat
    15601 gattgccagc acaagatact tattaatatc gccatccata ctttcatcat cacctccgct
    15661 gtagatagtc aggcagatat acaatgtggt gaaggcaaag ccgagtggca aaaacggagt
    15721 ctgcgaggac gcttcctgag aatcgtcttt attgcagtga atcacaggca aatgcggaag
    15781 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    15841 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    15901 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    15961 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    16021 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    16081 tgatgaattt aaagaaatga ttagtagaga tgcgttcctc gaacacgcag aagtttttgg
    16141 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    16201 ttttctcgat atcgactggc agggtgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    16261 gagtatcttt attttaccgc cgtctaaaat cgaactggac cgccgtctac gcggtcgcgg
    16321 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    16381 ttacgccgaa tatgattacc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    16441 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    16501 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    16561 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    16621 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    16681 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    16741 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggagcagca
    16801 agagcaggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    16861 acaaagcggg tcgcccttgt atctgtttga aagcctgaat cagctgattc aaaactacct
    16921 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    16981 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcat ccggtggcgg ttgcctgcat
    17041 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgatgtgat
    17101 tgaagataca cccgccacct accaggacat ggaacagctt tttggtaaaa gcgtcgccga
    17161 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    17221 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcattctcat
    17281 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    17341 tcgccgcatc gcccgtgaaa ctctcgaaat atacagcccg ctggcgcacc gtttaggtat
    17401 ccaccacatt aaaaccgaac tcgaggagct gggttttgag gcgctgtatc ccaaccgtta
    17461 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa
    17521 gattctttct gaaatcgaag ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg
    17581 tcgcgaaaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    17641 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattctgaca cctgttatcg
    17701 cgtgctgggc cagatgcaca gcctgtacaa gccgcgtccg ggccgcgtga aagactatat
    17761 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgattg gcccgcacgg
    17821 cgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    17881 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc actaccgcgc aaatacgcgc
    17941 ccagcgctgg atgcaaagcc tgctggagct gcaacagagt gccggtagct cgtttgaatt
    18001 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccagaagg
    18061 gcgcattgtc gaactgcctg ccggtgcaac gcccgtcgac ttcgcttatg cggtgcatac
    18121 cgatatcggc catgcctgcg tgggcgcacg cgtcgaccgc cagccttacc cgctgtcgca
    18181 gccgcttact agcggtcaaa ccgttgaaat cattaccgca ccgggtgctc gcccgaatgc
    18241 cgcgtggctg aactttgtcg tcagctcgaa agcgcgcgcc aaaattcgtc agttgctgaa
    18301 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgcgttggg
    18361 tggtagccgt aagctcaatg aaatcccgca ggaaaatatt cagcgcgagc tggaccgcat
    18421 gaagctggca acgcttgacg atctgctggc agaaatcggc cttggtaacg caatgagcgt
    18481 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagtca
    18541 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    18601 cccaattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    18661 ccatgaatcc tgccgtaaca tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    18721 tgaatgggat aaagagacgg cgcaggaatt catcaccgaa atcaaggtgg agatgttcaa
    18781 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgacct cgaatattca
    18841 aagtttgaat acggaagaga aagatggtcg cgtctatagc gcctttattc gtctgaccgc
    18901 ccgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    18961 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    19021 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    19081 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcatgc
    19141 tgtctggcct ggcagccgga tgcgcaccat ggcttcggca gctgcgggta gtaacagctg
    19201 ggtacaggtg aaaacgcacc gcaccattgg cgatgccgtc gctcatctga aaggccaggg
    19261 catgcagatt ctggcaaccc atctttctga taacgctgtt gatttccgcg aaattgatta
    19321 cactcgcccg acctgtattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcgtt
    19381 ggccctggcg gatcaggata tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    19441 ttccgttgcc tcagccctca ttctttacga agcccagcgc cagcggcaaa atgcgggcat
    19501 gtacctgcgt gaaaacagca tgttgccgga agccgagcaa caacgcctgc tgtttgaagg
    19561 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggt ctgccctacc cccacgttaa
    19621 tcagcaaggc gagattgaag ccgatgctga ctggtggtcc accatgcagg ccgcagggta
    19681 agcgcgatga aaggtcgcct gttagatgct gtcccgctca gttccctaac gggcgttggc
    19741 gcagcgctta gtaacaagct ggcgaaaatc aacctgcata ccgtacagga tttgctctta
    19801 caccttcctc tgcgctacga agatcgcaca catctctacc ccatcggtga actactgccg
    19861 ggtgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    19921 cggatgatga cctgccagat cagcgacggt tccggcattc tcactatgcg ctttttcaac
    19981 ttcaatgcgg caatgaaaaa tagcctggcg acaggccgcc gcgtgctggc ctatggcgaa
    20041 gcaaagcgcg gcaaatatgg tgcggagatg atccacccgg aataccgcgt acagggcgat
    20101 ctcagcacgc cagagttaca ggaaacgctc acgccggttt acccaactac ggaaggcgta
    20161 aagcaggcta cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacatgcgcc
    20221 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    20281 cgcactttgc accgcccacc gccgacgcta cagcttagcg atctcgaaac cgggcagcat
    20341 ccggcacaac gccggctgat tctggaagag ttgctggcgc acaacctcag catgttggcg
    20401 ttacgtgccg gtgcacaacg ttttcatgct cagccgctga gcgccaatga cgcgctgaaa
    20461 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    20521 gagatcgagc gcgatatggc gctggatgtg ccgatgatgc gtctggtgca gggcgatgta
    20581 ggttccggta aaacgctggt cgccgccctc gccgcgttgc gtgcgattgc gcacggcaaa
    20641 caggtggcat tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgt
    20701 aactggtttg caccgctcgg tatcgaagta ggctggctcg ccggaaaaca gaaaggtaaa
    20761 gcacgactgg ctcagcaaga agccatcgcc agcggtcagg tgcagatgat tgttggtacg
    20821 cacgccattt tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    20881 cagcatcgtt ttggcgtgca tcagcgtctg gcgttatggg agaaaggcca gcagcagggc
    20941 ttccatccgc atcagttgat catgaccgcc acgcccatcc cccgcacgct ggcgatgact
    21001 gcgtatgccg atctcgatac ctcggtgata gatgagctgc caccaggccg cacgccagtg
    21061 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    21121 tgtatgactg aagggcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcggaatta
    21181 ctggaagcgc aggcggcgga agcgacctgg gaagagttga aactggcgct accagagttg
    21241 aatgttggcc tggtacacgg acggatgaaa cccgccgaga aacaggcggt gatgacatcg
    21301 tttaaacaag gcgagctaca cctgctggtt gccactactg tcattgaagt cggtgttgat
    21361 gtacccaatg ccagtctgat gattatcgaa aacccggagc gtctgggtct ggcgcagtta
    21421 caccagcttc gcgggcgtgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    21481 aaaacgccgc tctccaaaac cgcgcaaatc cgcctgcagg tactacgcga cagcaacgat
    21541 ggttttgtga ttgcacagaa agatctggag attcgcggcc ctggcgaatt gttaggtaca
    21601 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    21661 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    21721 ctgatagaac gctggatgcc agaaacagaa cgttactcga atgcgtaaaa agcggcggtg
    21781 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacatcaaat acagcttaat
    21841 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacga ccaggaacgc
    21901 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    21961 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    22021 catcatgcgc caggtaacga agatggcgta caacgccatg aagatggtct gtaccaccag
    22081 aatcgccagc atcggcaacg ccagcgaagc cagctcccac agtttcagcc ccatcagcgc
    22141 catcgccagg aacaacgaca ggcttacgtt acccagcacg gataccgctc gctcaaagac
    22201 gcggtaaaag cccatcattg acagaccgtt gctcagaatc acgccaacaa atagtacaca
    22261 gacgaaggtc ggtagttcaa aagcagtacc agccaaaagt tgcgcaacaa ttttccccac
    22321 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accagcgagg tgatcatgcg
    22381 gcccacatcc ggcttttcga acgccgtcgg aacttcctgg tcatccggaa taccgttcgg
    22441 tgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    22501 caaaccgaac gttgcacagg ccatcgccac ttctgtcgcg ttggtgaaac cataacgttc
    22561 aatgaacaat ttactccatg cagcacccgt accgtgaccg ccggaaagcg taatagaacc
    22621 ggccagcagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    22681 attttgcatc accaacaggc caacaaccac aatcaggaaa acccccacca cacgcccacc
    22741 agctcgcaga ctggcaaggt tggcgttcag gccaatggtg gcgaagaaag ccagcatcaa
    22801 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtatcag
    22861 cagcgccagc gccactaata aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    22921 aaaggagacg gaatggacca acttgcgccc gagcagcaac gtcagcgttg cggcaacaag
    22981 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tgtccgcatc actctttcat
    23041 actcgtcata cttcatcgcg catttgcagt aaggcgaaat atgacgagta tcgagtcatg
    23101 ctttcctggt tcgaattctt cgctgaaaat agaaaaatgg catggatttt aagcagaaaa
    23161 caactaaaag ttatataaaa aagtacattt atctcattta aatatttaga tgaggatgtg
    23221 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    23281 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc gcagactcaa
    23341 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac tctgtttgcc
    23401 gcctgtcagc atttgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    23461 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    23521 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggttgttg
    23581 tctattcagg gcaccagctt caactttgtt gctccgctga ttatgggcgg cacagcgctg
    23641 aagaccggtg gtgctgatgt tcctaccatg atggcagctt tgttcggcac gttgatgctg
    23701 gcaagttgta ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    23761 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggttggactg
    23821 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    23881 ctgctgctgg caggcgtggt cttagcctta attatcctgc ttaaccgtca acgtaaccct
    23941 tacttacgcg tggcctcact ggtgattgcg atggcggccg gatatgcgct ggcgtggttt
    24001 atgggcatgt tgccagaaag caacgaaccg atgacacaag aactgattat ggtgccaacg
    24061 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    24121 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    24181 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtactggc aaacggcctg
    24241 aactcgtttg tctcggcggt atttaatacc ttcccgaact cctgcttcgg gcagaacaac
    24301 ggggtgatcc agttaaccgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    24361 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    24421 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    24481 gtttctcgtg agccgctgaa ccgccgggcg attctgatta tcgcgctgtc gctggcggtc
    24541 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    24601 ctgctctcct ccgggattgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    24661 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    24721 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    24781 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    24841 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa ctcgctgggg agcagaacat
    24901 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    24961 cgtttttccg cgccatctca tatcgtgctg aagaacgtca cgtttggccg tgatggccag
    25021 cccgcgaccc tggtagcaaa aagtgtcgac attgcgctaa gcagtcggca actgaccgaa
    25081 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    25141 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    25201 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    25261 gaagccggta aagtgctggg tacgaaggca cagattcagt ttagtgccgg atcgctttcg
    25321 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    25381 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    25441 aacgctgacg gcagctggca agtggaaaac ctgcgcatgg cggatatccg tctacaaagc
    25501 gaaaaatcgc taaccgactt ctttgcacca ttacgctctg tcccgtcgtt gcagattggt
    25561 cgcctggaag tcatcgacgc tcgtttgcaa ggtccggact gggcggtggc cgacctcgat
    25621 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggtaaactg
    25681 tcgatgaacg ccagcgagtt tatttatggc tcgctgcatt tatttgatcc gattataaac
    25741 gcggaatttg ccccgcaggg cgtagcactg cgccagttca ccagccgctg ggaagggggt
    25801 atggtcagaa cgtcaggaaa ctggcaacgc gacgggaaaa cgctgatcct cgatgatgcg
    25861 gcaattgccg gactggaata taccttgccc aaaaactggc aacagttgtg gatggaaacg
    25921 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    25981 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaat
    26041 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    26101 gccgccgcaa cgtttaatcg tgttgatgtc cgtcgtccgt cgctggcgct aactgccaac
    26161 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    26221 accgccagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    26281 gtgccggtga atattttgca acattgggga tggcctgaat taccgttgac tggcgacggc
    26341 aatattcagc ttaccgccag tggcgatatt caggtcaatg tcccgctgaa acctacggtt
    26401 agcgggcaat tgcatgccgt gaatgccgca aagcagcaag tgactcaaac catgaatgcg
    26461 ggcgtagttt ccagtggtga agttacgtcg acggagccgg tgcagtaaaa ataaggacgc
    26521 tagcggatat cagcgctagc gttccccgtt attagcgcaa aattccatac tcaccgcaca
    26581 ataaccattt caccggttca tcttcttcta tcgccggaaa acgtcctccc ggctcaagaa
    26641 aataattatc gtgttggtca tcatttacca tcgagatttc ccagccgaga acaaacgctt
    26701 tttccgccca gaagcgatga tacagacgcg gcggcaggca aatgccctgc cccggcttta
    26761 gcaccagcgt ttcaccaggt ttcatggtac gccgctgacc atcaacactc acggtaatga
    26821 ctcgttgatc atcacagaga ttggcttcgt tggcccaggc caactgcatg cataagtctc
    26881 caccgccccg attgaggata tcttccattt tgtgggtgtg aaaatgccag ggagtctgct
    26941 gatcttgttg gatctgcaac attttctcag catacggttt tggatatgcc gccgacccca
    27001 gcgcgccgtt gcgcatcgtt agcagtgtta gtcccgtttg gttgaaatca ccacgaccaa
    27061 aatcggtgat atcccaaccg agctgtaagt cgaccacttc ttgccagtta tcccgttccc
    27121 gctgcagcca gtcatccacc gtaaaatagg cgaactctgg caaacaaacg cggaaggtct
    27181 gcgcaatggc atgtgcttta tcaataacat aattaatttg tgaacgtttc attggcttct
    27241 ccgttgcgca tagcaagcca ggcagcgcca cgcattccgc tactgtcgcc gtgctgtgct
    27301 tttacaatgg gagtggtgaa ttgatcggtg aaaaccagtg gagcgacttc tgcgttcaga
    27361 tcggtgataa gcagttccac attcgaaagc ccgccgccga tcacaatcac gccagggtca
    27421 acaacgttaa cgatggttgc cagcgtgcgg gcaagctgtt ggcgaaaacg tgccacctgc
    27481 tgcatggcac aagcatcacc acgttgtgcc agggtgacaa tcgccgcagg agtgagcaag
    27541 ttaccagtca tctgttgata gcgttcacta aaaccgctgc cggaaacaaa cgactccacg
    27601 cagttgtgtt tgccgcaata gcaactgact gatggaccat cttcctgctc cgtatagcct
    27661 ggcaacgtga tatgaccgca ttcggcagca tttccccagg cccctataaa cggtcgttgc
    27721 ttgatggcaa tgccaccgcc gcagcccgta ccaagcgtaa taccaaatac cacgtcataa
    27781 tcttgcccgg caccgtcgca agcttctgat agcgcgaaac agttaccatc attggctatc
    27841 accaccggtt gcccaagcaa ttgctgcaaa tcagcctgta acgcatgacc gttaatcacc
    27901 tgaatattcg cgtttttaat caggccagtg agtggcgaga tactgccggg taaggcgatc
    27961 cctgtcagca tcggtcgttg aacgtcccgc ctaatctgct cgataagcgc cacaacgcat
    28021 gagacaaatt gttgatatgt cgacttttgc gtcgggcagc ggtaacggcg aatctcccag
    28081 ccatgcgcat ccatgacaac ggcggcgatt ttggtcccac caatatccaa ccccagatag
    28141 tgcatatctc gttccttgtt gcgtaaaagg cgggtcaccc cgcccttctg gcttagtaca
    28201 gtgatgcttt accgagcgaa ccgaacagtt tcattttgtg gcgaatgact tcccgcgccg
    28261 cgttaatcgc atccggatag atgacgttcg ggtcccacca gctctcttta gcgagaattt
    28321 cacgcacttt ctggaaatag gcgtatttca tatcgctgga gatattgatt ttgccgacgc
    28381 cgagcgtaac tgattcagca atctccgcgt ccgggtttgc cgaaccaccg tgtagcacca
    28441 gcggaatatc aatacgtccg gcaatgtcgc gcaaaatgtc catttgcagc ttcggctgca
    28501 tcccttttgg atagatgcca tgcgctgtac caatcgccac agccagtgtg tcggcacctg
    28561 tacgagcaat aaaatcggcg gcctgcgccg gatctgtgta ggtcacttgt gaaacaccac
    28621 cttctaccga cgttcctgtt tgtccgatgg ttcccagttc accctcaacc gacacgccaa
    28681 ccgcgtgagc gaggcgaacc acttctgcgg tcagcgccac gttttcttca tacggcagca
    28741 gcgaaccgtc gatcattact gaggtaaatc cgcagcgaat cgcacgcaaa acatgctcta
    28801 cagaggcacc atgatcgagg tgcagtacaa acgggaccgg actacgctgg gtaatttcgc
    28861 gaacgtgagc gaaaaaggca tcgcccacaa aatcatgttc gctgggatgg atggcaataa
    28921 tcgccggagt atttgtcgct tctgcttcat ccacgactgc gcgaaggaaa cagttatccg
    28981 ccacattaaa tgcgccaatg gcaaagccgt gctggcgggt aggttgcagt aattctttca
    29041 tcgaaactaa catgttaaat ctccagtttc agcgtgtagt ccgccctgcc ggtgcgttat
    29101 tgcactggca taaaggccat gacagaaaat gaatcaggct ttgccgatag cgccgctcgc
    29161 ctgtaaatac ggtgtaatcg cttcttcgaa ggccagttcc atagctttca gcgcgtccac
    29221 cagttcaatg gatatatcgt gacagagggt atgcaggagc gcggatttcc cccgttcaaa
    29281 aatggccgcc cccacgttga tttttgctac ccccagagtc gtacagagct ggagctgatc
    29341 gaacggcgta ccgctaccgc catgtaatgc cagaggcacg ctcgagcagt ggaaaatctc
    29401 cgccagacgc tgaaaatcaa tagcaggtac aacgccaggc gcgtacatgc cgtgcgccgt
    29461 gcctaccgag acggccagta gatcaatgcc tgtctgcgcg ataaacggtt ccacatcagt
    29521 gggttgcgtc atcaggtctg cattggcggc ctggtcgtaa cgcggcgcat ctgccagatg
    29581 gcccagttcg ccttccacgc agagctgcgc gctggcggcg agttccacca ccgccctggt
    29641 ctggcgcacg ttttcctcca ggggaaaagc agaagcatca atcattaatc cgctgaaacc
    29701 agcacgaaac gcctgacgaa tacagtccgg atttttgcca tgatcaagcg ccagagcgac
    29761 cggtactgag gcagattccg cgagcgtttt aaccactgcc gccgcgacgg gtgcaggaaa
    29821 atgcgccaga tgcccctgat agagattgag aatgatcgga gcgcgattct tctccgccac
    29881 gcggatagct gcgtgagcac tttccagatt catacagttg atcgccagca aggcgtaatg
    29941 ttcatgccag gctttcatca ccatactttt catatcggca aacatagggc ctccggttat
    30001 tcgagggtga aagtgatctc ttcatctttc agcttttgat cgctcagttc gtcaaactct
    30061 tcgtcgcgtt ccgttaccgg tttcttccag agtgaaagca tcacgccaca aatcaccgtg
    30121 cctataccca gcgccaggca aaacagcaga ggatgggtga acagaggcac aacaaacatg
    30181 ccgccatgcg gaaccggact ctccacgccc cacaccatga ttaagccgcc cgcgaccgcc
    30241 gatgccacca cacaagatcc cactactcgc agtaggtcac gcgcggcgat cggaatgact
    30301 ccttcagtaa tcatgcagat ccccatcgga aacgcggctt tcagcgcctc gcgctccgca
    30361 cgggtgtatt tatggcgtgt cagtaagaac gaaagtgtga tgccaaacgg agggatgatt
    30421 gagccgacaa atttcacggc ttctggtccg tagacgccac tgaccagcaa gccatcggaa
    30481 aacagcgaca tggttttgtt gaccggacca ccgaaatcga aggttgccat cgcgccaagt
    30541 atggccccca tcaggaagcg cgatccgccc tgcatcgact cgagcaaatg aatcagtgct
    30601 tcttgtaacc aggcgatggg tttaccaatc agcgtcatca ttaacagccc accgataatg
    30661 gtgcttaata ccggcaacac catgattggc atgagtcctt gcatcgactg cggcaggcgg
    30721 atatagcgtt taagtagcaa aatggtgtag cccaccagga aaccacccaa cataccgcca
    30781 ataaatccgg tatggatttg cccacagaga aagccgacaa tcaggcccgg ggcaaatccg
    30841 ggacgatcgg caatagaata ggcaattgcc gcgctaatca gcggcacaat cagccccatt
    30901 ccccagccgc caatctgatt aagcatccac gggattgttc ctgttttttc accaacatct
    30961 gtaccgccaa gaacttgccc caacgcaata catatcccgg cggccacaat aaggggtatc
    31021 atccacgaga tgccggttaa taaatgtttt ttaatttcct gtgaaaccgt aattttattt
    31081 tctttcatat taagatccct ccagtataag gatcatttgt ttcccagtga tttttcgacc
    31141 ttttcaagaa attgaacggg cgacttaatg acgatctctg tctttactcg cacaatacgt
    31201 ttattggtga agcgttcttc tccttttatt ttcacatcta cagcaagaat cacgacgtca
    31261 gcagcagaga tatctgccgc ctgtaattca ttttctgtac caatggttcc ctgggtttca
    31321 actttaatgg tatggcccag agcttttgct cctttgatta acttttcacg agcaatatag
    31381 gtatgagcaa tacccgccgt acaggcggca acacagacaa tattcatgac atactccctg
    31441 ttatttagca gacatcggat tgtgcatact gactaaagag atcaatgatt ttttgcggac
    31501 tcgtctcaac taacaattgt tctatgatgt catcatccgc cagcgcgccc gcgacctggg
    31561 aaagtaaacg gatatgggtg gtattttgat cttcaagacg taccgcaaat aaaataatac
    31621 agcgcacgct actgccatcc agcgtttccc agggaatatc ctggcgggta cggccaattg
    31681 ccagcgtagt attaatcaca gctgaagatt taccatgcgg gatcgcaata tgattttcaa
    31741 aaccagtcga gccttccgct tcgcgctgcc agacatcttt aataaaggtt tcccgacaat
    31801 cgatcgcacc atcttttttg agaagattag ttaattcttc aatcgcctcc gctttattcg
    31861 tcgcttgcat atctaaaata acgcgattcg tattcaggat ttttgttatg tccattcgtc
    31921 atacctcata aaattctgac attaaatttc aacgacccga gacgtcgcat gatgtaattt
    31981 ttccgtaatc accatttcag tgacccgctt aatatcggca tcattaaaaa aagcggtaac
    32041 ataagcgacc ggcatagtga ctgtaggcaa attaatggtc gaaataatca gttcgatatt
    32101 gtcaggcaaa acctgtgata aattcgctgc tgaaataaca tcaacaatcg tccattcagg
    32161 aaatgctcgc agaatacggc ttttcagtag atgcgaagtt ccgattccgg ttgaacagac
    32221 cagtaatacg cgtttacggg caatttgccg ttccatcgca gcctgaaaat gaacggtcag
    32281 gtaatccact tcatcctcgc tcaccgcatt ctcaccccag gttttaaata cctgattcac
    32341 cacatattgc gtcaaccgcc agacatccgc taattctgct ttaatatctt ccagcaatgg
    32401 attacggata tgaatacgat aatttagtcg gttaatcagc ggtttaatat gcaccaataa
    32461 gccatcatat aatgcgctgt cttcactaaa atcgcagtcc acgatgtcac tgaataccgt
    32521 aattaaacgc caggtaatca gacgcgcttc attgctggcc tgcatatgtg aaataatgct
    32581 aacatctttt tgtccatcaa tcgccacacc tgatgaaatg atatattgat aaataaacca
    32641 gacttcatca tcgggcaatg tatgtgcaat tcgttgttct atcttatgaa tcatctgact
    32701 ggcaacatta aagatatttt catcatattg cctggtttga tcttcttctc ttgataacgc
    32761 attcccgtgc gtgttgcgat acatcataat aaggatatga gtaaaaatgt taacgtaata
    32821 aggttctccc aaagaccaac ttaattcatt ttccatatcc agtaacaatg actggacaaa
    32881 taacacttct tcctctccaa aataatggac taatgcttta tagcttccgg gatccagacg
    32941 tgaatacacg acaccttgcg gctcattatg gttaataatg ccgttaatca gtaatgccat
    33001 tgcctgtcgg acttgccctt cactaccttc aatatgcgta ccacttgggc tgcggatcaa
    33061 cgataacccc aacggcgcaa gccaggactc aattactctc aggtcattaa caatagaggc
    33121 tccgctgata aaatagcgtt ctgacaattt actgatcgac gtttcattcg gtgtttcact
    33181 taataactgc gacgctattt taacgcgcct ggcattattc attacaccat caatctcttc
    33241 acattctgcc accaacaaat gatcaagatg cagcaaatca gcaatattct ccgcgcttaa
    33301 catcacaccc gcgccagcac gcttctcaac atttattctc cattgtccca gccattgctc
    33361 aagtaaacgt aaatcacgtt gaatggtttt ttccgaaaca tttaaatagc cggcaatcgt
    33421 aacaatagtc gtatattccc ttcgaggtaa aaggaatcgt aataatctgt tttgtcgcga
    33481 ggtaatcatt tgcatttttt acccttatca cagattaaga agccccatat ggaagagctt
    33541 cagttgccat ctctgagggt aaatataaag cagaataatt tcacaacaat actaaaatct
    33601 gtccgtaatc cggacagtga taaaacaaag caaggcggtt aagggatatt tttcagcaca
    33661 gatcacattt tcgtgatgaa taatcgtgat catgctcgca aatacgatgg aagaaatatt
    33721 tattaccaga tgtgagatta tttaccggat acgacacaaa accattcgta tccggcattc
    33781 ttcaatagat tacaacgtaa ttgtcagcgc attcccttga ggagtcacca ccagtccctg
    33841 ctcactttca gcctgcgaac cgccttgcag accatttact ttcacaatat tgcgcaagca
    33901 cagtgtccag ttcttcgcct cgcccgtacc agtcacagta atcgtgttgc cagtacgtgc
    33961 tgcttttaaa gtaaagatca ccgaaccgtc agcagtgggc acttcacaga cggcttcatg
    34021 cccgtcttgc agattaaaga ggtggaatgc cgtaccttcg tgccacgcgt aatcgggacg
    34081 ttgatcgttg ttgcccagcg ccagtagggt gctatcacgc acataaacgg gcagactcag
    34141 gaagctgtgc tgctgtttat gccagcgact accgtcgagt tcatcgttgt gccacaggtg
    34201 tgtccagcga ccttccggca ggtagaactg cacatcgccc gcttcagtga acacaggcgc
    34261 aaccatcacg ttgtcgccta acatgtattg acggtcaagg taatcacaag ccggatcgtc
    34321 cgggaactcc atcatcatgg cccgcatcat cggcgtaccc cgggcgtttg cacgtgcagc
    34381 ttcacgatac agatacggca tcatgcggca tttcagttgc gtgaagaagc gcaccacatc
    34441 acaggactca tcatcgtagg cccacggcac acgataagat ttgctgccat gtaaacggct
    34501 atggctggag agcaaaccaa acgcgcacca gcgtttgtaa acgtgcgccg gagcggtatt
    34561 ttcaaagccg ccgatatcgt ggctccagaa gccaaaacct gaaaggccaa tagacaaacc
    34621 accgcgcagg ctttccgcca ttgattcgta gttagcgtaa cagtcgccac cccagtgtac
    34681 cggaaatttc tgcgcaccaa cggaggccga gcgggcaaac aagacggctt cttcctcacc
    34741 aacggtgtcc ttgagcacgt tccacaccag ttcgttgtag atgtatgcat aatggttatg
    34801 cattttctgc ggatcggaac catcaaacca ttgaacatcg gttggaatac gttcgccaaa
    34861 gtcggtctta aagcaatcaa cgcccatcgc gaccagacct ttcagtttgt cggcgtacca
    34921 tttgcaggca tccggattgg taaagtcata aatcgccaga cctggctgcc atttatccca
    34981 ctgccacagc gaaccgtccg ggcgtttgag taaataacct ttctcttgta actctttaaa
    35041 gacgggggat ttttgcccaa tataggggtt aatccagacg cagattttca gccctttcgc
    35101 tttcaggcgg cggatcatcc cttccgggtc cggaaaagtc agcggatccc actcaaaatc
    35161 gcaccactgg aaggctttca tccagaaaca gtcaaagtgg aaaacatgca gcggcagatt
    35221 gcgttccgcc ataccatcga taaagctgtt taccgtcgct tcgtcgtagt tggtggtaaa
    35281 tgaagtggtt agccacagac cgaaggacca cgcgggcggc agcgccggac ggccagtaaa
    35341 acgggtataa cgatcaagta ccgctttagg cgtcgggccg tcgataacaa agtattcgag
    35401 atattcactc tcaacgctga actgtacttt ggagactttc tccgatccca cttcaaaaga
    35461 gacacattgg ggatgattga ccagtacccc ataaccacgg ttagtcacat aaaacgggat
    35521 atttttatac gcctgttcag tacttgtgcc gccgtcccgg ttccaggttt ctaccgtctg
    35581 accattgcgc accagggcag taaagcgctc tcccaggccg taaactgttt cgccaacgcc
    35641 aagatccagc cgttcaaaca tataatttcg ttgattattc gtgtcctgca cgtagccatt
    35701 atttttcacc tgactaccgg taatacgttc gccgttgcgc agaaaatcca gtgaccagaa
    35761 ctcacctttg ctgacacgcg cacttaagtt gccactttta aactcagcgt aacgttctgt
    35821 gttttcgata gtgaccttca cgtcctgcaa aatattgagc gggtaatgag gaccgttatt
    35881 cagcgccccc tgaaaatgct caatccgtac accgacaata ccttcctgtg gggagaaaaa
    35941 gcgcagcgta aataaaggcg tatcaagctg ccaggtacgt tcacgcacat cacgaggggc
    36001 agcatagacc accatttcat taccctgctg ttcaacctcg aacacctgaa gcgggtgaat
    36061 caaattgagg ccaggttgaa tcaaccagtt tccgtcgcta attttcattg ccgttcctta
    36121 gttctgcact tcttgagagg tgaattgttg ctggcaacgg cgtttgccct gcgccagctg
    36181 ttccataacg gttttcagat tgtgcgtcgt gagcgagtag tagcgtttag cgataatcgc
    36241 gctcagcaag taacagatcg ccggaacaat cgtgaatagc gcgataatga tgctaattgt
    36301 ggcgctgttc tgcgcttttt ctgccgcatc atatccgcca taagccagca tccagccgat
    36361 aagggcacca ccgaaggcca accccagttt gagcacaaac agcgtgccag caaaactgat
    36421 cccggtcagg cgtttaccat tacaccactc gccgtagtcg acggtatcgg acatcattac
    36481 ccactggata ggtgtcacca gttgatgcaa cacaccaatc acgaagatga agacaaacat
    36541 ggtgatgctg gcctgcatag gaacaaagaa catcgcgagg ctaatcactg ccagcagggc
    36601 gttcgtccac cagaagatag tgactttaca tttccagtcg gtcagcggtt ttgccagtgc
    36661 ggaaccaatc aggttaccca cgcaataagt ggtgagaaaa gcaacaaata cttccggcgt
    36721 acccaaaatc catgtgacgt aatacatcat cgccccacca cgtacgcaca ccgccaggat
    36781 attgaaaatg gtcaggaaac cgacaatacg ccactggtcg ttttgccaga tatcacgtaa
    36841 atcatcccgc atagacgtcg ttgtaggtgg tgcttcaacg cgttctttag tggtgaagaa
    36901 acagaatgcc agcatcataa atgccaccac ggaaaggacc gcgataccgc cctggaaacc
    36961 gagtggttta ttatcaccac caattaaatt aaccagtggc atcatcagaa cagtagagag
    37021 catgcctccc gcggtcgcca gcacaaaacg ccaggattgc agcgagatac gctgagtcgg
    37081 gtcattggtg attacaccac ccaatgcgca gtaagggata ttgacgatgg tataaagtaa
    37141 ggtaagtagg gtgtaagtaa ttgctgcata aatcattttt ccgttcatac tgagatctgg
    37201 cgtgctatag gctagtacac agacgatccc gaatggcagt gcaccaaaca gtacccacgg
    37261 ccgaaattta ccccagcgag agcgcgttcg atcggccaac aatcccatgc aaggatcgga
    37321 aatcgcatcc agtgcacgag cgaccaaaaa catggttccg acaaatccgg caggaatgcc
    37381 aaaaatatcg gtataaaaga acatcatata taacattacg ttatcgaaaa taatgtggct
    37441 ggcggcgtct cccatgccat aaccaatttt ctctttaacg gacaacactt cactcttcat
    37501 ctgcttttcc ttgatgctat aggggtgctg agaccgataa cgcattttgt aaaccatccc
    37561 tgcgagagcg aatattccga tatctggtta ttaatttatg attcttgttt tatgtgatcg
    37621 tggtagcgtt aatttcgctc atatatcatt gtaaaatatg agttttatat gaactataat
    37681 gctttcgtga taatacgctg cgtgtattag gcggaaaaaa ctgatctggg ggatgtagaa
    37741 actcaaggaa gtagctataa tgcgccccgc ctccatgtag caatcgaggc gcggaagatc
    37801 gtcgtctccg gtgaggcggc tggacttcaa atccagttgg ggccgccagc ggtcccgggc
    37861 aggttcgact cctgtgatct tccgccaaaa tgcctctcct gaactctccc gacgtcaaca
    37921 acactccttc ggaggcaaca tgtcagacaa agacttaacc cagcccccag caggcgagtt
    37981 tattatgttt gccagcggtg acggaagagt gcgtgttgaa tgtcgctttg agtcagacac
    38041 aatctggtta tcccaggcgg caatggctga actgtacgat aaagatgttc gcactattaa
    38101 cgaacattta atcaatatat tttccgaagg tgaacttgtt caaaactcaa ccatccggaa
    38161 attccggata gttcgccagg aaggaaaacg tcaggtttcc agagagatag accattacaa
    38221 cctcgaagcc attctcgctg tcggctatcg cgttcgctcg cctcgaggca cgcaattccg
    38281 ccagtgggcc acccagacgc ttcaggaata cctgatcaaa ggttttgtga tggacgatga
    38341 gcggctgaag aatccgcctg tcggttcatc ggttgtgccc gactattttg atgagatgct
    38401 tgaacgcatc cgcgatattc gcgccagcga gcgtcgggta tatttgcggg tgcgggagat
    38461 ctttgcttta gctgccgact atcagccatc gcttaaagaa accacgcaat tttttcaaac
    38521 catccagaat aaactgcatt ttgcctgtac cgggcatacc gctgcggaac tcatccacaa
    38581 acgagcagat gccagccagc cgcacatggg cttgaccagt tataaaggtg aagaggtacg
    38641 taagggcgac gtgacggtgg caaaaaatta tctcacccag gatgaggtca gcgaacttaa
    38701 tcgcgtggtt aatatgtggc tggactttgc cgaggatcag gcccgtcgtc gtcagcaagt
    38761 ctttttgcgc gactggcagg ataagctgga tcagttcctg caatttaacg accgagaggt
    38821 tctacagggc gctggtaaaa tcagcaagaa aatggccgat gaaaaagcgc aggcggagta
    38881 tgttcagttt gccgaacaac aacggcgctt aaaagaagcc gaaggcgaga aggatatcgc
    38941 tggtttgcta cagtggaata aagagtcgaa aaagtagccc ggaatccccc caaacgccat
    39001 acgcaaaacc atttgggggt acttatgggg ttactcattt atcaagttca aaatataaac
    39061 attaaatcaa taacttaacc atcaaatgcg actcctgtga tcgccaaaat gcctcttcag
    39121 tgatccccat cctgcttatt tctgagcaac ctgaccctgc tgatgtatct ggtagtatga
    39181 cagagtgatt catttttctt gccgagtgta cgaaaacgtc ttccttgcca ccgaccatca
    39241 accgtcactc aaaaaaacct ctcttttttc aagctatcaa gaataagttg cattttatct
    39301 gcaccggaca ccagccatcc atatatggct tttacctgct acaaagatga agaagtgcgt
    39361 aaatgtgatg attaaaacaa tcaaagaaaa attacaggtg caataatttc actataacat
    39421 tataatccat ggtgaaaata acactgcaca aggattgtca tcatcaaact catcggaact
    39481 attttttctg tcgtcattgg tgtaatactg attgtaagta tcctttcagg agattatttt
    39541 tgccgtgtgg ggcatggaac ttgtaaagca gccaacagcg ctaggcattt aattgaaagt
    39601 aaagagaaaa acagaagtag tcgcgtcaaa gtctttagca cacatattaa tttgagtcat
    39661 ccagcggcat cctggctagc aactcaaact tgcgctaaca atatattcaa tctgtttcaa
    39721 aacaataatg ccctttattt attcgaaaac gataataaaa ttgaactgcg aatggggcat
    39781 gattcttttt tctctattga aacgattgat aaaattaaca aagaaaacat caaaggaata
    39841 aaactaaaaa atgagacaac atatgtttct cttcctaatc caaccatttt aataaccaca
    39901 acatcaacac ctgaacgaca gttttctatt tctgttctgg atgatgataa caaagaaaat
    39961 ctacctaccg ataccgcacc ttgttttata attggtattc ctgatgaata tactaaagcc
    40021 atagagttat tgaacattaa ataattaaat ttcatattga gtttttagtg aggtttttta
    40081 aacaccatat gaataattgt tatatgtcat tatttcctcc attcactaaa actgattgat
    40141 tactctactc cctcaccgtc atcggtagtg gtatccttaa cgtatattga tgaaaagaca
    40201 aaacagtatg cagagggaaa aatgggttcc accagaaagg ggatgctgaa cgttctgatt
    40261 gccgccgtgt tgtggggaag ttcaggggtc tgcgcgcaat acatcatgga gcaaagccag
    40321 atgtcgtcgc agtttttgac tatgacgcgt ttgatattcg ccggattgat tctgctgacg
    40381 ctatcatttg ttcatggtga taaaatcttt tctattatta acaatcacaa agatgccatt
    40441 agcctactga ttttttccgt ggttggcgcg ctaaccgtac agctcacttt tttgctaacc
    40501 atcgaaaaat cgaacgcagc cacggcaacg gtgctgcaat tcctctcacc gactattatc
    40561 gtcgcctggt tctcactggt gcgtaaatcc cgccccggca tcctggtttt ttgcgctatt
    40621 ttgacatcgc tgatcgggac ttttttattg gtgacacacg gtaatccgac gtcattatcg
    40681 atctctcctg ccgcgttatt ctggggcatt gcctcggcat ttgctgctgc attctatacc
    40741 acctatccct caacgctgat tgctcgctat ggcacgttac cggtcgtcgg ctggagtatg
    40801 ctcattggcg gtctgatcct gctgcccttt tatgccagac aagggacaaa ctttgtcgtt
    40861 aacggcagtt tgattctggc gtttttttat ttggtggtca ttggtacgtc cctgacattt
    40921 agtctgtacc tgaaaggagc acaattaatt ggtggtccaa aagccagcat tttaagctgt
    40981 gcagaaccat taagtagcgc actgctctct ttgctgttgc tggggatcac cttcacattg
    41041 ccggactggc tgggaacgct gctgattctg tcatcggtga ttttgatttc aatggattcc
    41101 cgccgccgcg ccagaaaaat aaatcgtccg gcgcggcatg agtgagaatt accagccagg
    41161 caccgcgcct ccgttaaaga tggtttctgc cgctttagcg acttcgggtg attgataaga
    41221 ttgcagaaat tccttcacgt tttctgcatt cttattatct tcccgtgcca ccagaatatt
    41281 cacatacggc gaatttttat cttcaataaa tacgctgtcg ttaaccggag aaagtcctgt
    41341 ttgctggatg taagtggtgc tgataatcgc tacatccact ttcgggtcgt ccagtacacg
    41401 tggcaactgt gccccttcca gctccattat ctgcaaatgg cgtggattat cggttatatc
    41461 caacgcggta ggtaataacc cttttccctc tttcagagta attagcttct ctttttgcag
    41521 cagtaaaagc gcacgaccaa ggttagtcgg gtcgttgggg atcgccactg tcgcgccttc
    41581 ttttatttgc gccacggttt tgattttttt ggaataaccc gccatgggga agacaaacgt
    41641 atttcccacc gctaccagtt tataaccatg cgcctgatta tcttgttcaa gaaagggtcg
    41701 atgctggaag acgttcgcat caagctcgcc atgatttgtc gcatcgttcg gtagcaacga
    41761 accgctaaat cctaccagtt caacatcgag gccatatttc tcttttgcta cctttttagc
    41821 gacttccgcg acatcttgtt ctgcgccatt aataacgcca actttaatat gcttttcatc
    41881 gctactgctc tggtcgcaac ctgccagcag aactcctgcc agtaataatg cggcccccgc
    41941 ccgtagatga tgtgttgtca gtttcatttt tttatccttt tattaatgat tacctgacta
    42001 tagcggtaag gtcgctgcag tttaaaaaac gaaacgctat cgataagaat aaaaaggaat
    42061 aaaagtggaa tataaagtct ggcacttctt actgaccacg caaacacctt tcgtacagca
    42121 tgatgagagc ggcaaaagca aactacactt atgcttcatt cgatgcacat ttgcagaagg
    42181 ttgccgctat gaagccaacg atgctactca tgattaccat attcctgatt tttccagcca
    42241 tttctcaggc cgagtcgcca ttcagttccc tacagtcggc aaaagagaaa accacggtat
    42301 tgcaagattt acgtaaaatt tgtaccccgc aggcatcgtt atcagatgaa gcgtgggaaa
    42361 agttaatgtt gtctgatgaa agcaataaac aacacatacg cgaagccatc gtagcgatgg
    42421 aacgtaataa tcagagcaat tattgggaag cgttgggtaa ggtagaatgc ccggatatgt
    42481 aagaacggca acaaccactg accaacgctt ctttacctga tatcaggatt tcttcatttt
    42541 cacctttgca gtcaccaaca atgcagtcag caacatcaat gtgccggaca acatcagcgg
    42601 cgaagtcaga ccaatgttat ccaacgcata accgccgatt gccgcaccgc aggtattggc
    42661 aagctgaata acggccacct gaatagatcc ggctttttct gcctgatcgg ccagtgagcg
    42721 agtgatccac gttgaccagc caacgggaat taaagcaaag gtcagccccc agataatcgc
    42781 cacaccagta gcaacaattt tatcgcttcc ccacagcgtc agtaccagcg cgctcagggc
    42841 gagcacaaac ggtgcgcccg ctaaggccag tttcaccgaa cgtttgagaa taaacgacga
    42901 aagcgatgtc ccgacaaagc tggcgatacc aaaactcagc agcaccaggg ttaagccatc
    42961 aacaccgaat cccgccagat tcatatacac tgggcgaata taagtgaaaa aagcaaactg
    43021 tccggcaaag gacataaaga tggcgatcat cccggccatc actcccggac gttgtaataa
    43081 gcggaaagta ttttgtttct gatgcgaagg ttcgcctggc agcgaaggca gtgatttgat
    43141 aatccagaaa atacacagca cgcccatcac cgccgccgca ttaaagacat tgcgccaacc
    43201 gataagctcg cctaaaaaac tgcccaacgg cgcggcaatc accagcgcaa tagaaaccgc
    43261 gccgaagatc accgacagcg ccttcggcac cgtacgcggc ggtaccagac gcatggtcag
    43321 cgacgccgac atcgcccaga atccgcccag cgccagcccc agacaggcgc gaccgattaa
    43381 caacagactg aatgagttgg caaaggaaac cagcaaacag gaaagcgtca gcaaaacggc
    43441 gaacagaatg acaacgtagc ggcggtcagt ggcctgaatg gtctgggtaa taaacaaact
    43501 ggcaaacatc gccacaaaag cggttacggt caccgattgc ccggcaaccc cttccgaaat
    43561 gcctaaatcc tgggccattg gcgtcaacaa actgacgggc aaaaactcaa cgataataag
    43621 acaggcaacg caaaatgcca ccgagaaaac ggctgaccag ttcggtcggg tgatggcatc
    43681 cgcgccgcgg ttttcagcaa taaattcact catggtgtta cccgtggcat gtttcagagg
    43741 gaaagccgac cactttaaca ttgaaatgtg tgacgtattt aacgtttttg caacttttac
    43801 ggtgttgtgg gaaaagttcg aggctgctcg aagaaataaa aatcataggg caaggtgata
    43861 tgcattttcg cgattaggtt gggaataagg gataaagtat tgcacttggt gatatacttt
    43921 aataacagga tctcaacgat atgcgcgtgt ttaaaaccaa atggtttgca agggaagcca
    43981 ggtctcatgc aataacagat gaagagttgt gccgggccat tttagaaacg gaacaaggta
    44041 aagctgatgt acttggtggt ggcgttttta aaaagcgcct tcaccgaaat cgtgagcgtg
    44101 caattattct ggcgaaaggg ggaagcaatt ggttttacac gtttttatat gcaaaacagg
    44161 atatgtcgaa cattaatagt caggaactgg ctgggtttcg tgaacttgca aagcattatg
    44221 ccttcctgac gaaagcccaa ctgacagcaa tgattaacac taaagaactg acagagatct
    44281 gctatgactg caaaaactaa atttaaaagt cctgcatttg aagctattca cagcgcagcg
    44341 gctggattgt ccagagtcga tgctatttct gccgaaacga tgcgtacttt tgacaaagcc
    44401 tgcctgacca gcgtgcaaga cttacaacct gttgagataa aagcactgcg ggaaaaatta
    44461 aaagtgagtc agcccgtctt cgctcactat ctcaatacca gcgtttcaac tgtacaaaaa
    44521 tgggaaagtg gcgctaaacg ccccaatggc atttcattaa aactgctcag cattgtgcaa
    44581 aaacacgggc tggaggtgct gctgtaaata gaacgtagat aatgcgttca cctactgtca
    44641 attccgatac cggatgcgac gtacacgcct tatccaccca ccaggtcagg cgcggtctgt
    44701 aggctgataa gacgaagcgg ttttaatgca gccagtgaac gccctcggca ggctgaaaga
    44761 acgcgttata gcgcatctga aaagcattga tatcctgcat ttcaggttcg gtttcgcgca
    44821 gaaaacccag cgccagtctg acctgagcat cggtaatcgg cgcggcaagg cgggcttttt
    44881 gtaaaatccg atgccattgc agcaggttct gttcgctacc atcgacaatg acaatttgcc
    44941 agatgagcaa cacctgcgcg gcgttttgca ggtgcgactc atccgggcga tgcaaataac
    45001 gtaaaaattc gtcaccggct gactttccca tttgatcgac catcgactcc acgggtacga
    45061 catcgatgtt cagccgatcg ctgagacggc gaatcgcttt tctggccccg gatgtcagca
    45121 cccgaaaacc aaccacaaac actaccgcca gcgtggcgag cattatccag atcatgagtt
    45181 ctcctgcgta atcagaactc atgatatctg gaaaccctcg ccagttacta atccaccaga
    45241 ataatcttca gtgcaaacag agctgcgacc accaccacac acaggttcag atcgcgccag
    45301 cgcccggtgc acactttcat gatgcagtac gacataaagc ctagtgcaat cccttcggtg
    45361 atcgagaacg taaagggcat catcaccgtg gtaataaacg caggcaccga ttcggtaaaa
    45421 tcatcccagt taacgcgtgc caggctcgat gtcatcaata cgccaacaaa gattaacgct
    45481 ccggcggttg cgtaaggagg aaccatcgcc accagcggtg agaagaacat aaccagcaga
    45541 aacataacgc cgacaacaac cgcagtcagc cccgtgcgac caccgacagc cacacctgaa
    45601 gtactttcaa tataggcggt aacagacgag gtaccgataa acgcacccgc caccgaactg
    45661 atgctatcaa catacagcgc cttattcata ttggggaatt taccgttact atctattaag
    45721 ccagctttat cagtcacacc aattaatgtc cctgatgaat caaataagtt gatcagcata
    45781 aaggagaaaa tgataccggc gagttcaagt gataacgcac cgctcaaatc cacttcacca
    45841 atgacgccgc taatatcagg cggaatggaa tagacaccgc taaaatgaac atcaccgaaa
    45901 aataatccac agcaagacgt caccacaata gaaacaagca ccgcggcatg aaaatgacgt
    45961 gatgacaaca cagttataat aaaaaaccct aaaataccta acaacacgcc gtgagaactt
    46021 aaatcgccaa tcatcaccag ggtgtcttta ttggcgacaa taacgccagt attttttaat
    46081 cccattaagg cgataaataa tccgattccg ctggtgatac caatacgtaa acttaatgga
    46141 atgttggaga tcatccagta ccggatacga aacagcgtga gcaaaaatag cccaactgcg
    46201 ccccagaata tcgcgcccat cccggtctgc caggagatac ccatcgcccc cacgaccacg
    46261 aaggcaaaga aggcgttcag gcccattgcc ggagccagtg ccacgggtaa gttagcaaat
    46321 acccccatcg caatactgcc gataccggca atcaaacagg tggtaacaaa caccactttt
    46381 gggtccattt gtgccgcgcc gaggatttgc gggttcacaa aaacgatgta caccatggtt
    46441 aaaaaagtgg tcatccccgc aatcaattct gtgcggacgg tggtcccatg ctgaggtagt
    46501 ttaaataatc gcgaaagcgt ccctgattca ttactcacgt aatcggtatt gtcattattc
    46561 atttttttgt ccattggagg agatttaatc ccttgctttt gtaaaacaaa tgacaacaac
    46621 ggttcagtga taattatcac atttcaattg cacattaatg gatattcttt aataatctcg
    46681 cgacgtttct ttatgataaa taataatcaa attgataaaa tcaaaatgag aaaaatatga
    46741 ataattctat taaccataaa tttcatcaca ttagccgggc tgaataccag gaattgttag
    46801 ccgtttcccg tggagacgct gttgccgatt atattattga taatgtctct attctcgacc
    46861 tgatcaatgg cggagaaatt tccggcccaa ttgtgattaa aggacgttac attgccggtg
    46921 ttggcgcaga atacgctgat gctccggctt tgcagcggat tgatgcccgc ggcgcaacgg
    46981 cggtgccagg gtttattgat gctcacctgc atattgaatc cagcatgatg acgccggtca
    47041 cctttgaaac tgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa
    47101 tcgtcaacgt gatgggagaa gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa
    47161 ggcaaaacca gtacttacag gtcagctctt gcgtacccgc cctggaaggc tgcgatgtta
    47221 atggtgccag ttttaccctt gaacagatgc tcgcctggcg ggaccatccg caggttaccg
    47281 gccttgcaga aatgatggac taccctggcg taattagcgg gcagaatgcg ctgctcgata
    47341 aactggacgc atttcgccac ctgacactgg acggtcactg cccgggtttg ggtggtaaag
    47401 aacttaacgc ctatattgct gcgggtattg aaaactgcca cgaaagttat cagttggaag
    47461 aaggacgccg gaaattgcaa ctcggcatgt cattgatgat ccgtgaaggg tccgcagccc
    47521 gcaatctcaa cgcgctggca ccgttgatca acgaatttaa cagcccgcaa tgcatgctct
    47581 gtaccgatga ccgtaacccg tgggagatcg cccatgaagg acacatcgat gccttaattc
    47641 gccgcctgat cgaacaacac aatgtaccgc tgcatgtggc atatcgcgta gccagctggt
    47701 cgacggcgcg ccattttggt ctgaatcacc tcggcttact ggcacccggc aagcaggccg
    47761 atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg
    47821 agccgatcga tgcacaaacc ttacaggcgg aagagtcggc gagactggca caatccgccc
    47881 cgccatatgg caataccatt gatcgccagc cagtttccgc cagtgacttt gccctgcaat
    47941 ttacccccgg aaaacgctat cgcgttattg aggtaatcca taacgaactg atcacgcact
    48001 cccgctccag cgtctacagc gaaaacggtt ttgatcgcga tgatgtgtgc tttattgccg
    48061 ttcttgagcg ttacggacaa cgactggctc cggcctgtgg tttgctcggc ggctttggcc
    48121 tgaatgaagg tgcgctggcg gcgacggtca gccatgacag ccataatatt gtggtgatcg
    48181 gtcgtagcgc agaagagatg gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc
    48241 tgtgcgtggt ccgtaacggc caggtacaaa gtcatctacc gttgcccatt gccgggctaa
    48301 tgagcaccga cacggcgcag tcactggcgg agcaaattga cgccttgaaa gccgccgccc
    48361 gtgaatgcgg tccgttaccg gatgagccgt ttattcagat ggcgtttctt tccctgccag
    48421 tgatccccgc gctgaaacta accagtcagg ggctgtttga tggtgagaag tttgccttca
    48481 ctacgctgga agtcacggaa taataaaaaa aagcccggcg tcatgccggg caaaagtcac
    48541 cagttacgtt tatgccactg tcaactgctg aatttttttc tcgcggcgga ttttgcgttc
    48601 ttccatcacc gccactatcg ccatcaggca gatacaacca atcgcggcga tatccagcgc
    48661 ggcgaaggtg cctgcccagc cggtaaggcc aaataccggc gtcccatcgg caatcattcc
    48721 cagacctaac ttggcaaagc tgtcaccaat caggtaagcg aaggtgcctt taataccatc
    48781 ggcagcgcca atcgcttttt taggtacaaa gccaacagcc gccacgccaa tcaacagttg
    48841 cgggccaaag accaggaaac ccaacgcaaa gagagaagcc aggtagatat attggttgct
    48901 ggcgtgttga tacacaccga gcgtggcgat aatcagcgcc agcgcgatac aggccaccag
    48961 gccacggcga ccgttcgcca ggtcagagag ccagccccac agcagcgtac cgaccagcgc
    49021 accagcttca aacagcgtaa agccctgaat cgccaccgct ttagagagtt tcagttcctg
    49081 gaacgcatac acagttgacc actggtcgat accaatacgc accacataga ggaaaatgtt
    49141 ggcgaagcac agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca
    49201 cttggtcata tcggtagatt ctgtctcttt atcctcttca ctgatctcct cgccaaacag
    49261 ctcttcagct ttgccgaggc cataagattc cggggagtcg ctgccgtaac gcagaccgat
    49321 aaaaccaaca atcagcgcga taatcgacgg gaaaataaac atgccgatga catgaccatc
    49381 gaacaggtaa tttgcgccga acagcgccac acctgctgcg cctgcaccac caaggttgtg
    49441 agaaatgttc cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc atttggtgat
    49501 ggtggagtaa ctgcatgaac cgccggtact ctggaaaaag ccgctcaagg cgtagaaggc
    49561 aatcatcagg aacaggctaa ccgagccgct gcccatactg gcactgaagc ccagcataca
    49621 aatggcagac aggatcagca tgaacggcag gaattgtttg gtgtttttgc cgtcggcgta
    49681 gtaagaaacc agcgttttac ccacgccata agtgatggag aagcccaggc cgatcatccc
    49741 cagctgcgtc atgctcaacc cgtaggtcga gatcatgtcg ttctgcgcga tgttaaagtt
    49801 cttgcgaatc aggtacatcg taaggtagcc gataaagacc accaggtagg attgcatgaa
    49861 cggtttgaac cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcgaac
    49921 ctggtttaag aaagccagca tgggttactc ctgaaatgaa tacctgcgtg aggcatgcat
    49981 tgtaaaaatc agcagcaaat caggcctgag atagcgtcca ggcaaaacct ggaaaatttc
    50041 ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc acgcttctcg cggtgtctgg
    50101 gcgttcaaaa agggcagtaa cagcagtgcg gaaatcccgg cggcgatggc gataaccaca
    50161 aaaaatccgc tccagtgcca ggtatcgagt actttcgcca gcggccaacc agcaagcgac
    50221 gcaccaagat aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc ctctttgtgg
    50281 gaacactctg ccgccgccat gccgattaac atctgcgggc caaagacaaa aaaaccaatg
    50341 gtgaagaagc aggttgcctg catcacgtag ctggcaaatg gcatcagcca cagcgagccg
    50401 actgaaagca aaattccggc ggcgaaaatc aaattcatcg gcccgcggtt gccgttaaac
    50461 agtttgtccg aaccccaacc cgctaccagc gcaccgataa atccgcccag ttcaaacatt
    50521 gtcactgccg tattcgccgt gaccagatcg acgcccagcg tctcggacat atacaaattg
    50581 ccccagtcgt tgatcgccgc ccgaaccaca tagaccagca catagcaaaa cgaaagcagc
    50641 cagatatacg gattcagcaa cacatatttg gtgaggatct ctttacgcgt cagccctgcc
    50701 ccttcttgtt gttgagcaat ctccagtgcg tcgtgccgcc attcaccaac tgccggtaaa
    50761 cctaacgcct gcgggcgatc gcgcagccgc cagcagagaa aaatccccac gacgatcgcc
    50821 atacaacccg caatcatcat cccggcacgc cagccgtaat gcagcgcgct cgctgccatc
    50881 acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc ccaccaaccg
    50941 ccgcgctcgg tacgagaata ccaggcggtt aacagacgcg cacacaccgg tgaaccccag
    51001 ccctggaaaa aggcgttcaa cacccagagc acggcaaacg cccatagcga cgtcgagaag
    51061 ccaaacagaa tgttgataat gcccgtggca ataagcccta tccccataaa ataacgggca
    51121 tttgagcgat cgctgacaat gccggagaca aacttcgaca caccgtaggt aatgtaaaac
    51181 agggtcgcta acaggccaat atcgctacgg ctgagcacgc cgttagcaag gatttctggt
    51241 acggcggagt taaaactttt acgcgtgaag taaaacagcg cgtaacccag ccagatggtc
    51301 agcagaatat gccgacgcca gtagcgatag cgggcatcaa tttcatgttt atcagtcatt
    51361 aatggcgcat cggcaggcgc tttcagaaac ggcaacatca tcgaacctta gacataacgt
    51421 tgaggtagag aaacgctgac acgcgtgccg tgcaggcagg agatggtcaa tgtgccaccc
    51481 agcgccgtta cgcgctcgcg cattccggta aggccaaaac cgtgttgccc ggaatctggc
    51541 ggtaaaccgc tgccatcgtc ttcaatcacc agcattaacc gctcatcatg ctgccagcct
    51601 tgcagcgtga ccgcgctggc atcggcatgt ttcacaatgt tgttcaaccc ttcctggcag
    51661 acgcgaaata gcgtcacgcg ctggttttcg cttaacgctg attcatcgat tcgccattcg
    51721 agatgactga caatgccgcg cccttccagc tccatttccc gcatcagtga gcggatggcc
    51781 tgctccaggg tgagatcatc cagctggcgc ggacgtaacc gccctaacaa gcggcgcacc
    51841 gcgtcgtaaa cgcccaacga caactgttcg atgagctgcc cgctctgttt cacgctggcg
    51901 ttatccgccg cgagccgctg gacaatgcca gcctgagtac gaatagcagt gatggtctga
    51961 ccgatatcat cgtgcagctc tcgcgccaca tcgcggcgca cgctctcttc ggtttccagc
    52021 aaccgttcag cgagatgctg attgcgcgcc agttcctttt gcagcgactg gttaagttca
    52081 cgcaaccgct ggatgccagc gcccagtaac aaccctgtca gactttgcac cagcagcgag
    52141 agcaataaat ccaccggatg atcgcgccag gtttgactgg cgatcagcgc gatggcgttc
    52201 atcaatgtcg caatcagcgc tccttgccaa ccatagtgcc aggccagcgc gataatcggc
    52261 agcgccagac agaatggcgt aaagcgcgac agttcgtctg gcaatcccaa ctggagccag
    52321 agactgataa caaacagcag caagtaccag accagatgtc gcccgcgcca gttgattggc
    52381 tgagaaacca gtgacgggcc gagcggcagc caggtgttat tggcgagata gtgccagaac
    52441 accagacata tcggggccag cgtcaggccg ccagttaaag tcagcagcag cgcattccac
    52501 gactctttgc cgtgccaaag ccagggcaac gactgcaaca acgccgccgc cgttaacgcc
    52561 gcaccctgta acagcaaggt gcgccagtca cgttgatggc gatagcgcga gatcagcgcc
    52621 acgggcaata acgtcagtaa actaccgatc atcaataagg agaaatgggt taaaccgacc
    52681 gcctgcatta gccagtaaat cagcagccac tccgcaccca gtagcaccgg ccagtatccg
    52741 cgcgggcatt gcagcattag ccctagccgc aggccaaacg gaaataacag caccgccata
    52801 tcagggcgct caaccaggtg caggctgata ctccacaggc aaaaccatgc ggcagagaag
    52861 ataaaaaagc aggcaataac ggtaattaaa cgggagaaca acgtcttcat caccagccat
    52921 caaacatgcg gcgcgccagt tcaacgtcgt tactgacgcc cagtttttcc atcagattgg
    52981 cgcgatggac gtgtactgtt ttcggcgaca agcccagttc ggcggcaatc tctttcaccg
    53041 ccattccttg cgccagtttt tctgccacct ggcgttcgcg tttggttagc gggtcctggc
    53101 gaccagatgc cagtttaatg gcaatatccg gcgtcagata acagccgcca gtggcaaccg
    53161 tatgcaccgc agcaatgagt tcatccgggc tacagcgttt ggagagaaag ccgcgcgccc
    53221 ccgcgttaag cgcctgctca accagcgccg gactgtcgtg aacggagagc ataattgtcg
    53281 ccatgccttt cggcagctgg cttagaagct ccagaccgga gatatcaggc atggagatat
    53341 cgcaaataca cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg cgccccgaac
    53401 caaactcggc aactacctgc aaatcaggtt ccagccccag cagctgcgca aagccggagc
    53461 ggacgataag gtgatcgtct ataagggcaa cggtgatcat ggtcttgtcc tggcgggtaa
    53521 taaaacgcgc ttaccttaac gataagcgcg atgttgttca agccttgagc ggttactgcc
    53581 aaaagctgcg ctcttgttgc ttgtggaaag aagtgatgcc ttcaaggact tgtatatcga
    53641 ctaaccatta aaaatgctgg cggcattaat accttgttag tatccatttt gaataagtaa
    53701 aatgtatatt ctatatttat agtttgattg tttgtataat tataaattat tagttcagta
    53761 taaaaaccat actcgaaata attcgcgctg caggaaaaaa cgcagtgacg ttttgaacaa
    53821 cgcttgcact aatcctgaaa ggtcgaatat ttcgcagaca cagcacatgc aacttgaagt
    53881 atgacaagca tgcctgcttc attattacat tttaataata aaacccaccg attaatgtac
    53941 aaaaaaatcc ctgcgtgata ttgtctgtaa aaaaggagca gatatgcaca ctcaccctca
    54001 cttaataaaa caaactcttg ttccacgact cattaatgaa ataaacaaaa gaacacaaag
    54061 caatagtgag aagttatcaa acagtgataa attaacatat attaatgaca actactgtga
    54121 gataacacga aactacacgt cattttatgg catgaacatt ctgagcatcg ttcttttttt
    54181 acctatcgtt attttatgtt ttggctctgt tttattcttt atatatgaca ttatatttaa
    54241 cttcgaacga tacttagata cgtggagata ccgcgcagac tctgagttac tgctacatat
    54301 tgcttactgc gcaatttttt tcatgtcttt tgtttttgct gctatcctaa attattttat
    54361 ctttgccccg cgacattacc caattcgttt taatagaaaa acaggaaagg tctatatttg
    54421 taattatatt cttaatgata tgagtcataa atcgacctat accttctggt agcactaccc
    54481 attctacaaa catactaagc ctgaatataa agagtacgac tgggaacaaa ttcaaggtat
    54541 agcttttttt gcgcaaaatc accattccac atacgccacc attcgctgta ttgtttatga
    54601 atcattagac agtacaaata ttattgacac ctttaacctg ataacgacag agagtgctac
    54661 aaatgcattg catatgacaa ataacaaatt atggttatgg attaatagct ttatgaattt
    54721 tgacgatgag tttttagaga aatctatcaa cactgctcca ggtatttggg gccgtgaagt
    54781 gaattggcct gatgatatga ataagaaatc aatgttgtct tcaatggacg aatatatact
    54841 ctgaataatt tgagttgcag aatataagct cgcccggatg taaatatccc atccaggcga
    54901 gaaaatcact tactgaaaaa acaccgcaat cttgttaaac atcgtcgggt cggactgatt
    54961 acgctgcact ttcaccacat cttccagctt gtcgatttgg cttatcatct gctccagacg
    55021 ctggtcgtca ttgaccagta gccagatgtg gcttttgtcg ctgtcctgaa tcggcaggca
    55081 gagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
    55141 acccggatga ttgcgaacgg tgagctccag aatcacgtta tcatgagttg tgttttgcat
    55201 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtacacttt
    55261 ttcttcggca tcaatgcgca catggatcag cgccgggcca gggcgattga tgatttcctg
    55321 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
    55381 ggcaatctgc ataaagttga ttttgcccgg ataggtagcg gcaaaaacgc cctgcttgta
    55441 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca aaatgatttt
    55501 aacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
    55561 gtcgccggag aagcacaaca ctttgcggtc cgggttcgcc agcgccgcgc caatcgccgc
    55621 aggcaggcca aaacccatcg tgcccagccc gccggaggtc agccactggc gtgggcgatt
    55681 gagcggatag gcttgcgcgg tccacatctg atgctgaccc acgtcggtag tgataatcgc
    55741 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
    55801 cgctttcggg attggacacg ggaattcacg ctgcaaatcc gccaccaact ggtgccactc
    55861 tgcacgcggt tgcgcttcca ccagcgggat caactgcgcc agcacgtcat caacatccgc
    55921 ctgaattgcc acatgcggtt gcttgatttt gcccagctct gcgcggtcga tatcgacatg
    55981 aataattttg gcattcggac agaactgctc tgttttgcca atcgcccggt catcaaaacg
    56041 cgcaccgagt acaatcagca gatccgcctc ctgcaaaata tagttggtgc tgcgcacacc
    56101 gtgcatcccc agcataccca gcgacagcgg atgcgctttt ggcaacatac ccagcgccat
    56161 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcactc gcgcaggcgc
    56221 gttgatcaca ccaccaccca gataaagcac cgggtgtttg gcggcgttaa tcatcgccgc
    56281 cgcgtcacga atactttctt cgctaaaggc gggtgcggcg gctttttctg ccatagcggg
    56341 ctgtgtttca atctcaaaaa ctgccgtttg cacatcctta ggaatgtcta tccacaccgg
    56401 gccagggcgg cctgattgcg caatgctgaa ggcatcgctc atgacctgcg ggagttcttc
    56461 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
    56521 ttcctggaag gcgtcggtgc cgatcatcga ggcggaaacc tgaccagtga tgcaaatcag
    56581 cgggatggag tccagcctcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
    56641 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gggcgataaa
    56701 ccccgcccct tgttcgtggc gagccagaat atggcggatt tgcgtgcttt ggcttaaggc
    56761 atcgtaaaca ggcaggatag aaccgcccgg aatacccgtc acaatcttaa tgccctgctg
    56821 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtcg atgttgtgcc
    56881 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
    56941 aaaaaccccg cccggtttgc gccggcgggg ttttggaatc gtgtgttgtt ccagtcccta
    57001 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
    57061 tggtagtagt tttgcgttga acatggaagt agtcattagg gaccttgttg gttttgtgtt
    57121 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcttccgga
    57181 taaaaatacg tggcagatca cattttgaac tgaaactcag gcaataaagc atcatcacct
    57241 gaaagacggt caggttgttg acagagtgcg ccttattcat gtcgaataca gcgtaaacac
    57301 tttactctac caaccgcacc accactgttt ccggcaacat ttattgccgc ttttgtttac
    57361 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    57421 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    57481 aaaagcatcc gtgataatga aaaggcatag aaacgtcaat ttgttattga tgttggtatt
    57541 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    57601 gcgcgatctc aatgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    57661 ttacggtgtc tcacagctgt tttatggccc gatttccgac cgcgtgggtc gccgaccggt
    57721 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtct cgacctccag
    57781 tttgatggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    57841 aatggcgcgt actttgccgc gcgatttata tgaacggaca cagttgcgcc acgctaacag
    57901 cctgttaaac atggggattc ttgtcagtcc gctgctcgca ccgctaatcg gtggtctgct
    57961 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgccggtgt
    58021 gactttcagt atggcccgct ggatgccgga aacgcgtccg gtcgacgcac cgcgcacgcg
    58081 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgct atttgctgat
    58141 gctgattggc ggtctggccg gaattgccgc ctttgaagcc tgctccggcg tgctgatggg
    58201 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    58261 ggcatttttt ggcgcatggt ttgccggacg tcccaataaa cgcttctcaa cgttgatgtg
    58321 gcagtcggtt atctgttgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    58381 cgtgatgaat gtctggacgc tgctcattcc cgccgcgctg ttctttttcg gtgccgggat
    58441 gctgtttcca ctggctacca gcggcgcgat ggagccgttc cccttcctgg cgggcaccgc
    58501 tggcgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    58561 gatgttgccg caaaccggtc agggcagcct gggattgttg atgaccttaa tgggattgtt
    58621 gatcgtgctg tgctggctac cgctggcgac gcggatgtcg catcaggggc agcctgttta
    58681 agctcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    58741 tcacgcatca cccgcatatg cggcgcggag taagaataaa acgcctgata gcccgcacaa
    58801 agcacgttgt ctggtttatc ttcccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    58861 tggcagtcac ggcgcggttg cgacttttcg ctgccacacc atttttgtaa ggtggtgtca
    58921 aaaagttcaa tcgaaatacg actgatatct gcccgcaccc aacactcgaa cagggcaatt
    58981 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    59041 aattcaagat ggggaataag ctgtactgag cggatgccca gctgtttgaa attgtgataa
    59101 aaatcggcag gttgcagagc atccttatac tctacaacca gtttatgcct gtcctcttca
    59161 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    59221 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    59281 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    59341 gggtgtagtg ccagaggatc agcgccacaa tggcgaggat gccaatcgaa atccagaata
    59401 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    59461 gcatggtacg aaaccacgcc agtgatgtgc gttccggctg cagccctggg tcggcaatgc
    59521 gtctggcttt gcggctatcc ggcatacaac accagtccca tgacgatcac tgccacgacc
    59581 atcaatatta agctgatgat gaataagctg ttggtatagg gcaggtcctc tttcaggcgc
    59641 atcgcttttt cattgcgtaa ccagcgcaag tagccataca tcgctaaccc gccggaaaac
    59701 agacacaaca acaacgccag cagttcgcgg atgacgggcg tggcgaaatc tggcgcaagt
    59761 tgatcaagcc caacccccgc cgccagaaat cctagtgcag tacggatcca cgccagaaaa
    59821 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccgaggcg ggaaattttc
    59881 attgttgctc cttgtaaact gttcctcgcc gaaaaattag gccaggaaca gcaataatgc
    59941 atcagaactt ctgttttttc tcttccacct tcaccccttg tgtcggtaaa ccggtgtcat
    60001 agtcgcgtac cactggagaa taaccatcct gtgggcgtgg acgaaacgcc cccatccagc
    60061 gcggttgtgc atctttgcgc cacggacgca ggttccattg ataactgcgg aacggatcgc
    60121 gaattttgtc catgtaatcc aataaggcgt catgcatttt gctgcgaacg tcggcaaaat
    60181 ggatatcatc gatcaggtta tgcatttcat ttgggtcatt acggcgatcg taaagttcat
    60241 cactggtgaa gaggttgagc accagtttaa agtcatccgt tacccagcaa cgcaccggaa
    60301 taaaaccgcc aaagctgtca tgctcaatct cgtagcgatt aaattccacc aacacgccac
    60361 gtggttcttt tacggcaagg atattttccc ccggcagaat ctctggtttt tcaatatctg
    60421 ccagcgccat cattgtcggc agtaagtcaa tatgactgac tggcgtatcg acctgtcgcc
    60481 gctccccttg cggcgaacgg atgatcagcg gaatgcgggt gatgtcgtca tacatcgccg
    60541 ctcctttact gatcagctta tgtgcgccca tcatttcgcc gtgatcggag gtataaataa
    60601 cccacgtatt ttcacgttgc tctggcgtta aggcgttgat gacccgtccg atttggtcat
    60661 caacaaagtc attgcaggca aaatagagcg gatggtgata aagcccgtca tcaccgactg
    60721 gcgatggcat cgcctgcgcc cataagcggt gatgttccgg tttgttcgcc aggtcatcct
    60781 ctgctttctc acccagttcg tagtaaaaat cagtgtattt ctctaaatac tccaccggac
    60841 aggtgaacgg gtgatgcggc tcatcatacg aaatcaccat caggaaaggt tcgtcggcgc
    60901 gcgcgggctg ttgcagaaaa tccaccgccc gattgctgat gcggtgcgcc caggtgaagg
    60961 tttcgtcaat atggttcgcc tgtaaatctt cgacgctgtt taggccattg cgccacaggc
    61021 taatctcttt ttccgtcagt tcactaagat agttcgcccc atcgaaccag taatcagcgt
    61081 cccactccgg cggacactcg ccaggtgccg aaatagtcat gaccgtcgag atgccatttg
    61141 ccgatgtaac aggtgtgata gccggcatcc ttaaagtagc gccccatagt ggagatgttt
    61201 ttgcctggcg cgacgttgtt ggtccacggg ccggactggt tagcgtagat accggtgaat
    61261 agtccggcgc gtgcaggcgt acaaaccggt gaacaggtgt aggcggagtt aaagcgaata
    61321 ccttccgccg ccagactatc aatattttgc gtattcagcg gcttaccgct atagcaaccg
    61381 accatattgg tggcctgggt atcggtcatg atgaacagaa aattggggcg tttcataggc
    61441 tatcctttgc atcgggagta aaggttacgg ctgggtcgtg acgatgccgc cagctgtcaa
    61501 aaatcaggta gacaatcact acggcggcaa tgaaataact gatcatcgct aaccagcgcg
    61561 taccgtagcc gccgaattca gccagcccgg catagacgcc aatcatcgcg aacaggatac
    61621 caattgacgc agtcttgacg tttttccacg gtttcatgtc caccgcaaac gcatctttga
    61681 aggtgaacgg cgtggcgcgc ggtttgataa aaccgatcac cagcatcacg accacgttga
    61741 tgcagaacgt acaggccagc acatagagga aatggaagtc gaacttcacc agatagttga
    61801 tggtgatgta gctgattatg ccaatcccca tcgccacttt tgccgccagc gccggaatgc
    61861 gcgggaagaa aaagcccata atgatgatgg taaccagcgg cacgttgtaa ataccgttga
    61921 gctgtttcat ccagctatac agcccctgcg gcgcgttggc gatccacggc gcgaccatca
    61981 ccgaaacgat ggcgataaag aaaccaaatt tgcgcccgac ggtgaccagc tgttgcggct
    62041 cggcattctg gttaatgata cgccggtaaa tacccatact gaataacgta ctggcgctat
    62101 tcagaaagcc gttgaaggta ctgatcaccg caccaaataa caccgcaccg aagaagccca
    62161 ccagtggcac cggcagaacg ttattgacca gcgtcgggta ggccatgtcg gctttcggta
    62221 aatcctgata cagatgaaac gcaatcaacc ccggcagcac cagtaccagc gggtcgagca
    62281 ttttcagcac cgccgttaac agtgccccct tttgcccttc cgccaggctt tttgacgcca
    62341 gcgtgcgttg cacgatgccc tgattggtac accagtaaaa ggtgttaacc agaatcaaac
    62401 cggtaaatgc cgcgccaatc ggcaagggat cggtcgggcc accgactgag tttaatttct
    62461 cggcgtgaac ggtggtaagt tgctcaatgc cctgcataaa gctgcccttg cccatcgcga
    62521 tcaggccaaa taccggcacc atcaacccgc caataaccag cccaatacca ttgatggagt
    62581 ccgccactgc cattgcgcgc agtccgccga tcaccgcata caaaattccc gccagaccaa
    62641 gcaaaataac cagtaaccag atagccgcac catcggaaag ttgtagcgac tcacccacat
    62701 gaaacaggct gttgagcgcc aacgcgccgg aatagagaac aatcggcaga aagcagacgc
    62761 cggtggcaat gaggaagcag aagtcgatga taatacgcgt cgttttatca taacgttcct
    62821 ccagaaaatc ggggatggtg gcaatgccgc gcttcagata gcgcggtaaa aagattagcg
    62881 cgaggaagat cagcgtcact gcagaggtga cttcccagcc catcaccgac atgccgcttt
    62941 tgtaggcctg cccggaaagg ccgaccagtt gttccgtgga aaggttggtt agcattaacg
    63001 aagcggcaat aaccggcgct tttagtgaac gtccggcaag aaaatagcct tgttgcgatc
    63061 cggtatctgt tttacggacc ttccaccagg tgatgaccgc caccagcagc gtaaaaccga
    63121 caaaactcaa gatttgtaac gaattcatct catagccctt atttatgttt atgtcccgga
    63181 cgcatgaata tagggagagg taaaatgcgt cgggtcaggc agtcagattc ttaaaataca
    63241 aacgtcgtat ccctgaacgg attgttttct gttaagttca ggttgtgagc ataattcgcc
    63301 ggagggatat taaaatgaat ggaaaattgc aaagttcgga tgtaaaaaac gaaactccgt
    63361 acaatattcc cttattgatt aacgaaaatg tgatctccag cggaatttct ctgatatcgc
    63421 tctggcatac ctacgccgac gagcattatc gggtgatctg gccgcgggac aagaagaaac
    63481 cgcttatcgc caactcatgg gttgcggtgt ataccgtaca aggatgtggg aaaattcttt
    63541 taaagaatga cgaacaaata actctgcatg gcaactgtat tatattttta aagccaatgg
    63601 atattcactc ttatcactgt gaaggtttgg tctgggaaca gtactggatg gaatttaccc
    63661 ccaccagtat gatggatatt cccgtcggtc agcaaagcgt tatttataat ggcgaagttt
    63721 ataatcagga acttaccgaa gttgctgagt taattacttc accagaagca ataaaaaata
    63781 atctggcagt cgcttttctg acgaaaatta tttatcagtg gatttgtctt atgcacgcag
    63841 tcggtaaaaa agatccacaa cggcggcaaa ttgaaaaatt aattgccact ttacatgcca
    63901 gtctacaaca acgctggagc gtagctgata tggctgccac gatcccctgt agcgaggcct
    63961 ggttgcgtcg tctgttttta cgctataccg gcaagacgcc gaaagaatat tacctcgatg
    64021 cgcgtctgga tctggcgcta tcgctattaa agcaacaagg gaactcggtt ggcgaagtcg
    64081 ctgatacgct caacttcttc gactcctttc atttcagcaa agcctttaag cataaatttg
    64141 gttatgcgcc gtcagctgtg ctgaagaata cggacaggta ataaccagaa tggcgagtgt
    64201 gtaaatgact cgatgatgtt cagaaatacc aggcggagta ccatttacgg cgactccacc
    64261 gggaaaagag ttaaccgata ctccagaaga gcaccgccag taattggggt gtgataatgc
    64321 gcaggaacat cactaacgga tagacagtgg cgtaagagag cgccgctgca ccgctggttg
    64381 gatgaagatt attagcaaac gccagcgccg gaggatcggt catggaacct gccagcatcc
    64441 cgcacatggt caggtaattc attttggcta acatccgcgc cagaatacca acagtaatca
    64501 gcggaacggc ggtgatcagg gcaccataac caatccagct tagcccttcg ccatccacca
    64561 gtgtatggat aaaatcgccg ccggatttca ggccaacgac cgagaggaac agtacgatcc
    64621 ccagctcccg cagcgcgagg ttggcgcttg gcggcataaa ccagtacagc ttgccgatac
    64681 tgccgatacg cccgagaatc aacgccataa tcagcggacc gcccgccagc cccagtttca
    64741 acgcggccgg aaatcccggc acaaagacgg gaatagaacc taacaatacg ccaagcccga
    64801 taccaataaa cactggcagc atctgaacct gttgcagttt ttgctgcgca ttccccagca
    64861 cattggcaac ggcatcaatt gcggacggac gccccaccag gttgaggata tcgccgaact
    64921 gcaggctgat atcgctactt gcaactaatt caacaccagc acggttcagg cgcgagataa
    64981 caacgtcata gcgttcttta aagtgcaggt cgcgaatacg tttaccgagc acgttttcat
    65041 tggtgaccac cacacgctcg acgcgcaaat cagtgccttt cgtagacagc gaggtatcga
    65101 cctcctgacc aatcaccagt tgcgcattat gtaaatccgc aggctgcccc accagatgca
    65161 gcaaatcgcc cagttgaata acggtttctg gcgaaggcac ttttagggtt tcttctcgtt
    65221 tcaatcgtga gcaaataact ttatcgccat tgagaatcgg cacatcttta atggctaaat
    65281 tatgcaaatt aggattctca actcggatgt taatcgtcct gattagcgca ccgccgttgg
    65341 ttcgcgtgga ctcgtgctgc tgggcctcag tctcaacatt gacgcggaaa ataacccgca
    65401 gcatccacat agtgaacaaa atgccgcaaa tcccaaatgg atacgccatc gcatagctca
    65461 tccccatttg atcgaccatc gccatcggtg tccccaggtc gcgcaagatc tgctgccctg
    65521 cccccagcgc tggcgtattg gtgaccgcgc cggaaaaaat ccccagcacg accggcagtg
    65581 gaatatcaaa cagtttatgc aggatggcgg taaccagacc accgatgatg acgatcagaa
    65641 cagcaaacag gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag cccggcccta
    65701 cctgaatccc gatggtataa acgaacagga tcaggccaaa ttcctgaata acatgcagca
    65761 tatcgctact taatgtcatc cccgcctgag aaacaaaatg gccgacgatg atcccgccga
    65821 acagcacgcc gccaatgccc aatccaacac cgcgaaactt gacgttgccg ataaacaacc
    65881 cgacgaccgc caccaaagcc agaatactga ccgttaatgc tatatcactc atcgtccatt
    65941 tctcttgaaa taacatttta attgctacgg attatggctg aatcggtgaa gcagatttgc
    66001 cttatagcgc acattatcac gggaaagaac gtgccgaaat atcttaaaca ggcgaaattt
    66061 aacgcattgt tgaagaaaaa agacaaaaaa aaagccccac cagaatggcg gggcaaagag
    66121 aatagctagt tagctattta acgcgggacg ttcgctgata gcgatacgct gcgctgcgat
    66181 gggttcaggc tcattacgaa ttaaa
//
LOCUS       DAOFSV010000009        62081 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.009, whole genome shotgun sequence.
ACCESSION   DAOFSV010000009 DAOFSV010000000
VERSION     DAOFSV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.009"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(35..196)
                     /locus_tag="QBH27_000656"
     CDS             complement(35..196)
                     /locus_tag="QBH27_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDQ7013017.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(323..928)
                     /gene="osmY"
                     /locus_tag="QBH27_000657"
     CDS             complement(323..928)
                     /gene="osmY"
                     /locus_tag="QBH27_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDQ7013018.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(1321..2910)
                     /gene="prfC"
                     /locus_tag="QBH27_000658"
     CDS             complement(1321..2910)
                     /gene="prfC"
                     /locus_tag="QBH27_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDQ7013019.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(3001..3678)
                     /gene="yjjG"
                     /locus_tag="QBH27_000659"
     CDS             complement(3001..3678)
                     /gene="yjjG"
                     /locus_tag="QBH27_000659"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDQ7013020.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGVITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(3693..4139)
                     /gene="rimI"
                     /locus_tag="QBH27_000660"
     CDS             complement(3693..4139)
                     /gene="rimI"
                     /locus_tag="QBH27_000660"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDQ7013021.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(4108..4521)
                     /gene="holD"
                     /locus_tag="QBH27_000661"
     CDS             complement(4108..4521)
                     /gene="holD"
                     /locus_tag="QBH27_000661"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDQ7013022.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4624..5655
                     /gene="rsmC"
                     /locus_tag="QBH27_000662"
     CDS             4624..5655
                     /gene="rsmC"
                     /locus_tag="QBH27_000662"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDQ7013023.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLNRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANSVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            5855..5941
                     /locus_tag="QBH27_000663"
     tRNA            5855..5941
                     /locus_tag="QBH27_000663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5889..5891,aa:Leu,seq:cag)
     gene            5970..6056
                     /locus_tag="QBH27_000664"
     tRNA            5970..6056
                     /locus_tag="QBH27_000664"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6004..6006,aa:Leu,seq:cag)
     gene            6091..6177
                     /locus_tag="QBH27_000665"
     tRNA            6091..6177
                     /locus_tag="QBH27_000665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6125..6127,aa:Leu,seq:cag)
     gene            complement(6216..6452)
                     /locus_tag="QBH27_000666"
     CDS             complement(6216..6452)
                     /locus_tag="QBH27_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDQ7013024.1"
                     /translation="MLQRTLGSGWGVLLPGLLITGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            6593..7381
                     /gene="fhuF"
                     /locus_tag="QBH27_000667"
     CDS             6593..7381
                     /gene="fhuF"
                     /locus_tag="QBH27_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDQ7013025.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCDDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHAIFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(7419..8096)
                     /gene="bglJ"
                     /locus_tag="QBH27_000668"
     CDS             complement(7419..8096)
                     /gene="bglJ"
                     /locus_tag="QBH27_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDQ7013026.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(8054..8779)
                     /locus_tag="QBH27_000669"
     CDS             complement(8054..8779)
                     /locus_tag="QBH27_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ7013027.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            9400..10170
                     /locus_tag="QBH27_000670"
     CDS             9400..10170
                     /locus_tag="QBH27_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDQ7013028.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            10161..10634
                     /locus_tag="QBH27_000671"
     CDS             10161..10634
                     /locus_tag="QBH27_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDQ7013029.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            10741..11280
                     /gene="dnaT"
                     /locus_tag="QBH27_000672"
     CDS             10741..11280
                     /gene="dnaT"
                     /locus_tag="QBH27_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDQ7013030.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            11283..12020
                     /gene="dnaC"
                     /locus_tag="QBH27_000673"
     CDS             11283..12020
                     /gene="dnaC"
                     /locus_tag="QBH27_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDQ7013031.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            12069..12563
                     /locus_tag="QBH27_000674"
     CDS             12069..12563
                     /locus_tag="QBH27_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDQ7013032.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            12817..15108
                     /gene="opgB"
                     /locus_tag="QBH27_000675"
     CDS             12817..15108
                     /gene="opgB"
                     /locus_tag="QBH27_000675"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDQ7013033.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLTWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(15248..16270)
                     /gene="lgoD"
                     /locus_tag="QBH27_000676"
     CDS             complement(15248..16270)
                     /gene="lgoD"
                     /locus_tag="QBH27_000676"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDQ7013034.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEVVGLGKNVADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            16409..17323
                     /locus_tag="QBH27_000677"
     CDS             16409..17323
                     /locus_tag="QBH27_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ7013035.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFEQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENMRYAH"
     gene            17540..18901
                     /locus_tag="QBH27_000678"
     CDS             17540..18901
                     /locus_tag="QBH27_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7013036.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(18950..20614)
                     /gene="tsr"
                     /locus_tag="QBH27_000679"
     CDS             complement(18950..20614)
                     /gene="tsr"
                     /locus_tag="QBH27_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDQ7013037.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLHDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVMPAAPRKMAVADSGENWETF"
     gene            20991..23141
                     /gene="btsT"
                     /locus_tag="QBH27_000680"
     CDS             20991..23141
                     /gene="btsT"
                     /locus_tag="QBH27_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDQ7013038.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            23191..23394
                     /locus_tag="QBH27_000681"
     CDS             23191..23394
                     /locus_tag="QBH27_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDQ7013039.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            23405..24361
                     /gene="yjiA"
                     /locus_tag="QBH27_000682"
     CDS             23405..24361
                     /gene="yjiA"
                     /locus_tag="QBH27_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDQ7013040.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVISTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(24395..24697)
                     /locus_tag="QBH27_000683"
     CDS             complement(24395..24697)
                     /locus_tag="QBH27_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDQ7013041.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(24684..24971)
                     /locus_tag="QBH27_000684"
     CDS             complement(24684..24971)
                     /locus_tag="QBH27_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDQ7013042.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIISARKADRKERSRYEHG"
     gene            25168..26334
                     /locus_tag="QBH27_000685"
     CDS             25168..26334
                     /locus_tag="QBH27_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDQ7013043.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIRDQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRGMVTREGMSYCITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            26398..28017
                     /locus_tag="QBH27_000686"
     CDS             26398..28017
                     /locus_tag="QBH27_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDQ7013044.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTEDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            28007..29311
                     /locus_tag="QBH27_000687"
     CDS             28007..29311
                     /locus_tag="QBH27_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDQ7013045.1"
                     /translation="MGSKWCIYRLGDIAEFSYGKMPKKEFVGTGDYVIFSGYKYTESY
                     PEKNTSAGELIVVARGVGGTGDVKITTRDCYLTNLSIKINLDETKVRKEYFYYLFLQS
                     NLRYLDSGSAQSQITINDLANVEISAPSLNVQDLIVKSLKAFDDKITTLSSMNQTLEQ
                     MSQTLFKSWFVDFDPVIDNALDAGNPIPEALQTRAKLRQKVRNSADFKPLPAEIRSLF
                     PSEFEETELGWVPKGWKEGTLPEIAFINSTSWTNKNQPDYINYVDLSNAKDGRIYSIE
                     KLSFNDAPSRARRILKKDDLIFGLVRPANRSFAYVHVDGLTGSTGFAVIRAQKTIYKN
                     FIYYFVTYDKNIEELARIADGGAYPAIKPDDICTLPLIIPSINIIEKFDELTSNFREM
                     MNNSLIQNEYLTNLRGTLLPKLISGELSLEDLPDLTTDTEAA"
     gene            29406..32669
                     /locus_tag="QBH27_000688"
     CDS             29406..32669
                     /locus_tag="QBH27_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDQ7013046.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYMNPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERNNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSSLN"
     gene            32884..33222
                     /gene="symE"
                     /locus_tag="QBH27_000689"
     CDS             32884..33222
                     /gene="symE"
                     /locus_tag="QBH27_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDQ7013047.1"
                     /translation="MTDTHSIALPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPAIEESELMQSLRQVCKLSVRKQRQVQEF
                     IGVISSKTPR"
     gene            complement(33265..33429)
                     /locus_tag="QBH27_000690"
     CDS             complement(33265..33429)
                     /locus_tag="QBH27_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDQ7013048.1"
                     /translation="MEFYENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            33606..35018
                     /locus_tag="QBH27_000691"
     CDS             33606..35018
                     /locus_tag="QBH27_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDQ7013049.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCGSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRTNCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(35261..36217)
                     /locus_tag="QBH27_000692"
     CDS             complement(35261..36217)
                     /locus_tag="QBH27_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDQ7013050.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLEYASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPATARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFNEFISELIRRMPQHRERIMTIAERIHNDGWLLGRERGRKEGKEEGEKSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKRDPWIEY"
     gene            36684..37916
                     /gene="mdtM"
                     /locus_tag="QBH27_000693"
     CDS             36684..37916
                     /gene="mdtM"
                     /locus_tag="QBH27_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDQ7013051.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATIALSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANTIVARFIK
                     DPTEPRFIWRAVPIQLAGLALLIVGNLLSPHVWQWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            37957..39237
                     /locus_tag="QBH27_000694"
     CDS             37957..39237
                     /locus_tag="QBH27_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDQ7013052.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARTDAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHHVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            39353..40504
                     /locus_tag="QBH27_000695"
     CDS             39353..40504
                     /locus_tag="QBH27_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDQ7013053.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLHKTVEERFGHE
                     ISEDALRDAIVLKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRHQHNIPYIAIETDYSTSDIGQLSTRVAAFIEML"
     gene            40514..41281
                     /locus_tag="QBH27_000696"
     CDS             40514..41281
                     /locus_tag="QBH27_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013054.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            41278..41535
                     /locus_tag="QBH27_000697"
     CDS             41278..41535
                     /locus_tag="QBH27_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDQ7013055.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            41600..42460
                     /locus_tag="QBH27_000698"
     CDS             41600..42460
                     /locus_tag="QBH27_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDQ7013056.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            42528..43706
                     /locus_tag="QBH27_000699"
     CDS             42528..43706
                     /locus_tag="QBH27_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7013057.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSMLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(43719..44273)
                     /gene="kptA"
                     /locus_tag="QBH27_000700"
     CDS             complement(43719..44273)
                     /gene="kptA"
                     /locus_tag="QBH27_000700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDQ7013058.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKVGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            44523..45206
                     /locus_tag="QBH27_000701"
     CDS             44523..45206
                     /locus_tag="QBH27_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013059.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            45203..45664
                     /locus_tag="QBH27_000702"
     CDS             45203..45664
                     /locus_tag="QBH27_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDQ7013060.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            45677..46849
                     /gene="iadA"
                     /locus_tag="QBH27_000703"
     CDS             45677..46849
                     /gene="iadA"
                     /locus_tag="QBH27_000703"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDQ7013061.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            46914..47825
                     /gene="hypT"
                     /locus_tag="QBH27_000704"
                     /gene_synonym="qseD"
     CDS             46914..47825
                     /gene="hypT"
                     /locus_tag="QBH27_000704"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDQ7013062.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(47818..48210)
                     /gene="iraD"
                     /locus_tag="QBH27_000705"
     CDS             complement(47818..48210)
                     /gene="iraD"
                     /locus_tag="QBH27_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDQ7013063.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(48207..48290)
                     /gene="idlP"
                     /locus_tag="QBH27_000706"
     CDS             complement(48207..48290)
                     /gene="idlP"
                     /locus_tag="QBH27_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDQ7013064.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            48882..49668
                     /locus_tag="QBH27_000707"
                     /pseudo
     CDS             48882..49668
                     /locus_tag="QBH27_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(49809..50582)
                     /gene="uxuR"
                     /locus_tag="QBH27_000708"
     CDS             complement(49809..50582)
                     /gene="uxuR"
                     /locus_tag="QBH27_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDQ7013065.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(50797..52257)
                     /locus_tag="QBH27_000709"
     CDS             complement(50797..52257)
                     /locus_tag="QBH27_000709"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDQ7013066.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(52338..53522)
                     /gene="uxuA"
                     /locus_tag="QBH27_000710"
     CDS             complement(52338..53522)
                     /gene="uxuA"
                     /locus_tag="QBH27_000710"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDQ7013067.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            53862..55205
                     /gene="gntP"
                     /locus_tag="QBH27_000711"
     CDS             53862..55205
                     /gene="gntP"
                     /locus_tag="QBH27_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDQ7013068.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(55379..56281)
                     /gene="fimH"
                     /locus_tag="QBH27_000712"
     CDS             complement(55379..56281)
                     /gene="fimH"
                     /locus_tag="QBH27_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDQ7013069.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSAHDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(56301..56804)
                     /gene="fimG"
                     /locus_tag="QBH27_000713"
     CDS             complement(56301..56804)
                     /gene="fimG"
                     /locus_tag="QBH27_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDQ7013070.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATFDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(56817..57347)
                     /gene="fimF"
                     /locus_tag="QBH27_000714"
     CDS             complement(56817..57347)
                     /gene="fimF"
                     /locus_tag="QBH27_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDQ7013071.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNDIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(57357..59993)
                     /gene="fimD"
                     /locus_tag="QBH27_000715"
     CDS             complement(57357..59993)
                     /gene="fimD"
                     /locus_tag="QBH27_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDQ7013072.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(60060..60785)
                     /gene="fimC"
                     /locus_tag="QBH27_000716"
     CDS             complement(60060..60785)
                     /gene="fimC"
                     /locus_tag="QBH27_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDQ7013073.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(60822..61361)
                     /locus_tag="QBH27_000717"
     CDS             complement(60822..61361)
                     /locus_tag="QBH27_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7013074.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(61426..61974)
                     /gene="fimA"
                     /locus_tag="QBH27_000718"
     CDS             complement(61426..61974)
                     /gene="fimA"
                     /locus_tag="QBH27_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDQ7013075.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT        15177 a        15878 c        15279 g        15747 t
ORIGIN      
        1 ggctttggat aaaattttct taatgatctg aaatctaggg tcgttttcgg cccatgaaca
       61 aactcaccag gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg
      121 taccggccag accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga
      181 tgccccaacg aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc
      241 gtgaaaatca ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt
      301 cgggaggacg aattacgacg aattacttag ttttcagatc atttttaacg cttttcacac
      361 catctaccgc tttggcgata ctttcagcac ggtcgctctg tgcctgggaa tcgacggtac
      421 cggagagctg aaccacgccg tcggtggttt caactttcac atgacgggaa gggacgatat
      481 cgtccgccag cagtttggct ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct
      541 tcacagagcc ttctttagcg tcgcgaacgt gcagtttgtc gctgacagag gtcacccctt
      601 caacgccttt cgccactttc actgcctctt cggcctgagc ctggctttca acgaaaccgc
      661 tcagggtcac gactttttga tcggttttga cagagatatc ggtgctcttg atgttgtcat
      721 gatccaccag ggccgccttc actttcgcgg tgatggcgct gtcatccatg aaattaccga
      781 ctttattcat agagctatcg actttttgcc ctgcgctttc attggtagtc tgcgcgttgt
      841 tttccgcgta ggcagagcct gtcgcgacgg cagaggtcaa cattacagcc agcagagttt
      901 tcgaaatctt cagtcttgtc atagtcatcg atttattcct gtaagtttgc tcgtaatttg
      961 agcctggcaa cacgaggttg cattgctgaa tagggagaga cttcaccctc tacagaagtc
     1021 aatggtcgcc atcacaaaag cgatgggtga tgaataacga ccattacagc ctctgaatca
     1081 gttattaata tcggtagaat gacaatctac ggctttagat actgatatct acgcattgaa
     1141 cggtatttaa cgccgtcaga aatgtcatta ctttgttaaa tatagatcac aattttgaaa
     1201 ccgctcggaa tatcatcgaa aacataagca aaagtgaatg ttttaagaac attccgtaag
     1261 cggctaataa ggaagggaaa ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa
     1321 ttaatgctcg cgggtctggt ggaactgaac gtccggataa cgttcctgcg ccaggcgcag
     1381 gttgaccatg ctggtagcga tgtaagcgag gttatcgccg ccatcaagcg ccagttggct
     1441 ttcgttctta cgcttgaact cttcgaattt cttcgcgtct gcacattcta cccagcgggc
     1501 ggtggcaacg ttgactgatt catatactgc ttcaacgttg tattcgctct tcaggcgcga
     1561 taccaccaca tcaaactgca gcacaccaac cgcaccaacg atcagatcgt tgttggagat
     1621 tggacggaac acctgcaccg cgccctcttc ggaaagctgt accagccctt tgagcagctg
     1681 tttttgcttc agcggatctt tcaggcggat gcgacggaac agttctggcg cgaagttcgg
     1741 aataccggtg aacttcatca tctcaccctg ggtaaaggtg tcgccaatct gaatggtgcc
     1801 gtggttgtgc aggccgagga tatcgcccgg atatgcttct tcaacgtgcg aacggtcacc
     1861 tgccataaag gtcagcgcgt cggagatcac cacatctttc gcagtgcgca cctggcgcag
     1921 cttcatgcct ttttcatatt taccggatac cacgcgcata aacgccacgc ggtcgcggtg
     1981 tttcgggtcc atgttggcct gaattttaaa tacgaagccg gtaaatttgt cttcgctcgc
     2041 ctctacggta cgggtatcag tctgacgcgg catcggcgca ggagcccact ccaccaggcc
     2101 atccagcata tgatcgacac cgaagttacc cagcgcagta ccgaagaata ccggagtgat
     2161 ttcgcccgca aggaacagct ctttgtcgaa ctcattagac gcgcctttca ccagttccag
     2221 ttcgtcacgc agctgctgtg ccagatcttc accgaccgct gcatcgagat ccgggttatt
     2281 cagcccttta acaatgcgga cttcctgaat ggtgtggcct ttaccgctct gatagagata
     2341 ggtttcgtct ttataaaggt ggtaaacgcc tttaaacagc ttgccgcagc cgatcggcca
     2401 ggtgatcggt gcgcagccga ttttcagctc gttctcaact tcatcgagca gttccatcgg
     2461 gtcgcggata tcacggtcaa gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg
     2521 ggtaacttcc atcagcttgc gggtacgatc ttcaacacct tttgcggcgt cgataaccat
     2581 cagacagcag tccaccgccg tcagggtacg ataggtatct tccgagaagt cttcgtgccc
     2641 cggggtgtcg agcaggttaa ccaggcaatc gtgatacgga aactgcatca cagacgtagt
     2701 aatggagatc ccacgctgct tttccatctc catccagtcc gacttagcgt gctggttgga
     2761 accacggcct tttactgtac cggcggtctg aatggcctgt ccgaacagca gcaccttctc
     2821 ggtgatggta gtcttaccgg cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc
     2881 cacctcttgc aaataaggag acaacgtcat aattctttct tcttgagtaa gcggcatcgc
     2941 gccgcgcttg ttgaaaacga aaaattgcgg ctattttacc catcaacggg ggggaggcaa
     3001 tcagtgttta cacaggagct gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc
     3061 gatgccttct ggttgctcgc gatggtgcgc attcagccag caggtcgcaa gcccggcgtt
     3121 gatgccaccg agaatatcgg actcggcagt gtcgccaacc atcagcacgc gtgaacggtc
     3181 aggattgccc gcctgttcca gcgcataatc gaaaattttc ttattcggtt tggcaacgcc
     3241 aacttcttcg gaaatcacca gcaaatcgaa gtaatcacgc aggcccgtgc gttccagacg
     3301 cacttgctgc aaggcgctaa agccgttggt gatgatgccg attttagcgt tgccacgaat
     3361 ggcgttaagc agggaaaccg cccccggcaa cggcgtacag atttccgcca tcgcattaat
     3421 aaaggcctcg ttgagtttac ccggttcgac gttcagccgt tcggcccagc tctcaaaccg
     3481 cccgtgctga agctgtaatg aagtgatcac gccgttttga taatccaccc acagtggctt
     3541 gttaacggcc tgatagtcct gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa
     3601 catccgctgc aggccggtga atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa
     3661 aatccagtcc cacttcatta ttccaccttg tcttacatac tgattggtaa cgccatgatg
     3721 atggcgtctt cgcgaccgtc cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta
     3781 aagcctaaac tttcgtacag ggcaatggcg gcagcatttg aggcacggac ttccagccat
     3841 agcgtcgcca cgccgcgttt ttccagttcg tcgatcagat gttccagcag cgcccttccc
     3901 agtccctgac gctgatagtc aggatcgacc gcaatattga acaatgtagc ttcatccagc
     3961 accacttgcg taatcgcaaa cgccgccatt ttgccgtttt gcgttaactg aaagttgaga
     4021 tagcgctcgc cctggttgct ggcaaaggtt ttttcactcc acggaaaggt gtgggcgcgt
     4081 tgttcaatgt ggaaagccgc tggtaaatca gtcgtttcga gggaagaaat cgtgttcata
     4141 tgtgcaaatt tgttgccata acgcggcgcg tgccgttggg tttgcccgta attcggtgag
     4201 cgccggtgat gtcacctgag cgccttccag tgatagcggt tcgtcagtac ccaaccgcca
     4261 gctgttgcag cggctgcctt gcggcagcat cgcgattttt tctggcgtca gttgcagcac
     4321 ttgatcgggg ctgacggtta acgcacgcag aacatcgctc accagaggat cggtcagggc
     4381 gggaagatcg tttgccacca tcaccagacg gacgtgtggc gggatcgcaa tggcaatctc
     4441 cccctgcaac gcgccagggc gacgcagcga ccactgggta atgcccagtt gctgtaactg
     4501 ccagtctcgt cgggatgtca tagcgaatcg ctcctgttgt caggggcgca aatatagcaa
     4561 attcgtcgat accgcgccaa catatggcta taatcgccgc cagtatcaat tgaggagcat
     4621 tccatgtctg catttacccc ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa
     4681 caaagccgta ttctgtttgc cggagactta caggatgacc tgcccgcgcg tttagatacc
     4741 gcggccagcc gtgctcatac ccagcaattc caccactggc aggtgttaaa ccgccagatg
     4801 ggggataacg cccgttttag cctggtcgcc acggcggatg atgtcgctga ttgcgatact
     4861 ttgatttact actggccgaa gaataaaccg gaagcccagt tccagttgat gaatttactt
     4921 tctctgcttc cggtgggcac ggatattttt gtcgttggcg agaaccgcag cggcgtgcgc
     4981 agcgccgagc agatgctggc cgattatgcg ccgttgaata aggtagacag cgcccgtcgc
     5041 tgcggcctct attttggtcg tctggaaaag cagccggtat ttgatgccaa taaattctgg
     5101 ggcgaataca gcgtcgatgg cttgacggtc aaaacgctgc cgggcgtgtt tagccgcgac
     5161 ggtctggatg tcggcagtca gttgttgctc tcgacgctaa ccccgcacac gaaaggtaaa
     5221 gtgctggatg tcggctgcgg cgcaggcgtt ctttccgttg cttttgcgcg ccattcaccg
     5281 aagatccgcc tcactctgtg tgatgtttct gcaccagcgg tagaagccag tcgcgcaaca
     5341 cttgctgcca acagtgttga aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa
     5401 ggtcgttttg atatgatcat ctccaacccg ccgttccacg atgggatgca aaccagcctg
     5461 gatgcggcgc aaacgctgat tcgcggcgcg gtgcgtcatc ttaatagcgg cggcgagctg
     5521 cgaattgtag cgaacgcctt cctgccttac ccggacgtgc tggatgagac atttggtttc
     5581 cacgaagtga ttgcgcaaac cgggcgcttc aaggtgtatc gcgccattat gacccgccag
     5641 gcgaagaaag gttaagtatc tgatatctgt cgccgcatac cgtgttggat gcggcgtaca
     5701 cgccttatct gaccgacaaa cgaattatcc acaatccgct gatggacatt tttccagcaa
     5761 ttacacctct gtcgataatt aactattgac gaaaagctga aaaccactag aatgcgcctc
     5821 cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat tggtagacgc
     5881 gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc ccctcgcacc
     5941 aaaaaccacg ttgatattgc tcgcactggg cgaaggtggc ggaattggta gacgcgctag
     6001 cttcaggtgt tagtgttctt acggacgtgg gggttcaagt cccccccctc gcaccaaacg
     6061 aggcaatatc aaaaaaagta agatgactgt gcgaaggtgg cggaattggt agacgcgcta
     6121 gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct cgcaccaatt
     6181 atctttactt cttttcttgt ctcttcactg ttttatcacc cctgattcaa attcattatc
     6241 atcagcatga tgccagcaat aagcgccagg cacgatggta gcaaaacgta atgccgtaac
     6301 tgtttgtgat acagcatcac cggcgtcaat accaatccca tcagaatgac aatccgccat
     6361 tgatccggcg ataaatccgc atacatcagc cctgtaatca gcaatcccgg cagcaacact
     6421 ccccagccac tgcccagcgt acgttgcaac atggtttcat ctctttcatt gataatgata
     6481 accaatatca tatgataatt tttatcattt gcaagccaga gaaatccctt gctatcggat
     6541 aaacctatcg ctaggattag caatcattat catttagatt actatcccga ttatggccta
     6601 tcgttccgca ccgctctatg aagatgttat ctggcgaacg catctccagc cacaggatgc
     6661 tgggcttgca caagccgtac gtgcgacgat cgccaaacat cgtgaacatt tgctcgagtt
     6721 tatccgcctg gatgaacccg ctccgcttaa cgccatgacg ctggcgcaat ggtcatcacc
     6781 gaatgcgcta agttctctac tcgcggtcta ttccgatcat atctatcgca accaaccaac
     6841 gatgatccgc gagaacaaac cgctgatctc actatgggca caatggtata tcggcctgat
     6901 ggtgccacca ttaatgctgg cgctactgac gcaggaaaaa gcattagatg tgtcgccgga
     6961 acatttccat gctgagtttc acgaaaccgg ccgtgccgcc tgtttctggg tcgatgtgtg
     7021 tgacgataaa aacgcaacac cacattcgcc gcagcagcga atggaaacgt taatcagcca
     7081 ggcgctggtt ccggttgtgc aggcactaga agcgaccgga gagatcaacg gcaaacttat
     7141 ctggagtaat accggttatt tgatcaactg gtatctcact gagatgaagc aactgctcgg
     7201 cgaggcgacg gttgaatcgc tgcgccatgc catctttttt gagaaaacgc tcacgaacgg
     7261 tgaagataat ccactctggc gtaccgtggt actgcgcgac ggcctgctgg tgcgccgcac
     7321 ttgctgccag cgttatcgct taccggatgt gcagcaatgt ggcgattgta cgctgaaatg
     7381 aaagcactgc cggggaagta aacccggcat catgcggatt aatagggatg caacacatta
     7441 cttgcttcgc aatgccgcat acataacaga atatctgcgg cctccaacaa tcctgcatca
     7501 gaactgacgc ccagtttcga catcacatta aatttatgcg cgcggatcgt tttgatattg
     7561 cgtttaagct gctcggcaat ttgtgtcatt gagtagccac gcgacataaa gcgcaatatt
     7621 tctctctccg tcgggcttag cgtccggctt tggttaatgt accactgatt gttcagtcgg
     7681 tcggtcgcct gacgtacacc atttaatgac aaaaagagtt cctgatgaaa aatctccagc
     7741 gttgacgctt tacttaatac accgtccagc ggtgatggcg acaatgaacc aatcagccga
     7801 gcttcaatat catcatccgc aataactaaa cgccgggtac gcggaaactt aatcgccagt
     7861 tcagtcaggc aagataatcc ctctctgcgc tcacttctca tggcagaaaa agaaaaaatg
     7921 accgccgcaa acgagatccg cgacatggca tcctgaaatg actcctgact ggtataggta
     7981 tggagcttat aagggttgcc cgcaaacttt ctgaataaac tctcaatacc gatactactc
     8041 atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg ttccattctg
     8101 cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg ctatttatcc
     8161 atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg tttttttggg
     8221 cgctgatagt tttattactt ttcttaagca atgaagctat ctggttgatg ccccatcctt
     8281 tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag cgatagctaa
     8341 aatcatgagt ttcagtcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg
     8401 tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg
     8461 tcgccgtagg gcacatgagc agttctactg cctgggaata ccaggagcgt gacaccatga
     8521 aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac
     8581 gtggatcttc actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta
     8641 ataaggtaag gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtcc
     8701 tcatgacctc ttttaaccct gcttgcataa caggtatttt actgataaca attccatttt
     8761 tgcagcatcc tggcaacata tttcctccac atccactgtc taaaaattat gatggtttat
     8821 tcaggatatt cattccttaa atgtttccag caaattaata gattaagcga ataaattatc
     8881 cttagcattc actttcatat cagcatactc ctgctgcaca acaactccac tgcatgtgtc
     8941 agaaggttag catcaaatga taacctggtg aaatagcctg gagacattta aaatatctac
     9001 attaagttgt taggaatact taatgaaaaa cagaaatcac attcatttaa ttataaatta
     9061 aatgatatcc cttcccgcag aatgcgggaa tcacacaatc aatatttatt caataaaatc
     9121 tgatgatttt aacttgttaa cactaaaaaa cgcgcccagc tatctattat cctgacccgt
     9181 aaaggctaaa acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc
     9241 gagagataat tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa
     9301 ttttgatctt aacctacctc aaaacaaccg tgcttcatga tacaaatgga ttctcattat
     9361 ctgaacaata cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga
     9421 gccgtaacgc ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc
     9481 gcgttggttg atgagctttc ctcacgactg ggtcgggcgt tgggaatgga cagcgtcgaa
     9541 agttctatct cttcgaacgc catagtgctg acaactatta aagatgggca atgcctgaca
     9601 tcgacacgaa aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac
     9661 attgtgattc ttgcggagca tcatctgctg gattacaaag gcgtagagaa acgatttagc
     9721 caaattcagc cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc
     9781 gcctgtttct gtaaactcaa taacggtggc tgggatggtg ccgtcatcac cttctttgcc
     9841 agtacgaccg cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccacagatc
     9901 aacttttgcc ttaccgcttt cgccgccacc accatttccg gattgctttt gcaactcccc
     9961 actttcagcg atacacccac cattgcgatg gccgccagcg ttctgctgct ggtgccgggc
    10021 tttccgttga ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca
    10081 cgctgggcga tcgccagtct gctgacgctg gctacctgcg tcggcgtggt gatggcactg
    10141 acgatttggg ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata
    10201 tgatcctcgc cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgtgcggg
    10261 cgttacgctg gtgtgcgctg cttggcgcga ttggccatgg ttcacgaatg atcttaatga
    10321 ccagcgggct gaatattgag tggtcaactt ttatggcttc tatgctggtc ggcaccattg
    10381 gtattcaatg gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta
    10441 tccctatgtt cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc
    10501 agttaggtta cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat
    10561 cgattgttgg tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc
    10621 gccctcgcgt ataaaattgc ctcgcttaag cacacggatg agagacagcc tcctctcctc
    10681 cgtgtgttac tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt
    10741 atgtcttcca gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac
    10801 caaaccgttc tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg
    10861 gcgttttatg ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg
    10921 gcgcgtccgg gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct
    10981 cccgttgctg tccccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgac
    11041 tttatccgcc tggcggcgct atggggcgtg gcgctaagag agccggtgac caccgaagaa
    11101 ctggcctcat tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg
    11161 caacaaaaac tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa
    11221 cgagatgtga atacggtcag cgaacctgac agccaaattc caccaggatt cagagggtaa
    11281 cgatgaaaaa cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca
    11341 aacctgcatt caaaacgggt gaagagctac tggcctggca gaaagaacaa ggggcgatcc
    11401 gctccgccgc tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct
    11461 ccggtattcg tccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag
    11521 ggcagatgaa tgcgttaagc aaagcgcgcc agtatgtcga agagtttgac ggcaacatcg
    11581 ccagcttcat cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct
    11641 gcaacgagct gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt
    11701 cggcgatgaa agataccttc aggaatagcg gtaccagcga agaacaactg cttaacgatc
    11761 tgagcaacgt tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg
    11821 aaaaagtgat catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga
    11881 tgctgaccaa cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc
    11941 gtatgcgcct gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccgtg
    12001 taacaggtaa agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt
    12061 gagtaatgat gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat
    12121 taatttccac cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg
    12181 aacttggcaa ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta
    12241 acttgcttag cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca
    12301 ttctgcaata ctgcgcgaag caaaagctgg cttcggtaac cgatgccgaa aacatcaaga
    12361 accaggtgct ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc
    12421 tggatggcat tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca
    12481 tcggaacgac tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc
    12541 aggggctgaa cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga
    12601 cgcggctctc tcctgccctt ttccctgctc ctggacggtt ttacccctgt tacacgggaa
    12661 gcgatcgcga tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg
    12721 acactagaat tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac
    12781 gttggctaaa cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc
    12841 ctttttctcg cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt
    12901 gcagccacat taacagtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc
    12961 gactatttta ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg
    13021 accggtgctg gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca
    13081 gcggtgttcg gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt
    13141 ggttacagcc tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt
    13201 cgtcagataa cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct
    13261 tattataaag agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctat
    13321 ggcgaaagtc tcgagcgaac ctattttgat aacgaggctt tcccggatct cacgcctgaa
    13381 ctgggcgcgt tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg
    13441 gattacacga ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt
    13501 gaaggcaacg cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat
    13561 atcctgaaaa actcgggtta tcagaactat ttcgtgcagg gcgcaaatct gcgttttgcc
    13621 ggtaaagatg tgttcctgaa atcgcacggc ttcgaccact tatacggctc agaagagctg
    13681 aaaagcgtgg tagccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt
    13741 ctcgatgaag cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg
    13801 tttaccctga cagtcgatac ccatcacccg gatggtttta tttctcgtac ctgtaaccgc
    13861 aaaaaatatg attttgacgg taaaccgaat cagtcattca gcgcggtaag ttgcagccag
    13921 gagaatatcg cgacgtttat caacaaaatc aaagcgtcac cgtggtttaa agatactgtc
    13981 atcgtcgtct cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag
    14041 caggatcgca ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg
    14101 gcagtgaagc gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat
    14161 aactatctcg gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc
    14221 aatatcaaag agaaaacatt gacgtggaag ccagatatca tccgcctgtg gaaattccct
    14281 aaagagatga aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc
    14341 catttccgtt tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa
    14401 agcgaatact cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc
    14461 gtctgggttg accgttgcta taagatggca caactctggg ctccggaact ggcactttcc
    14521 accgactggt gtgtctcgca ggggcagctt ggcggtcagc aaattgtcca gcatgttgac
    14581 aaaacaacat ggaagagcaa gacggcattt aaagatacgg tcatcgacat ggcgcgttac
    14641 aaaggcaatg tcgatacgct gaagattgtt gataacgata ttcgctacaa agccgacagt
    14701 ttcatcttta acgtcgccgg tgcgccagaa gaggtgaaac agtttagcgg gatttcccgt
    14761 ccggagtcgt ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac
    14821 aagcatccgc tgccgaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac
    14881 gccagccgtc ctattccggt acgcgtaggc aatgaagaac aaactcttgt gctgggcaat
    14941 gaagtgacca ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt
    15001 gtgccgccgg aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag
    15061 ctcgggatcg gcatggtgga aattaaagtt gtggaacgtg aagggtaatt cctgactgat
    15121 gtgtaggtcg cataaggtgc gacctacaca ccttctccgg cctacaaaac atcattgaga
    15181 aacagaaaaa aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct
    15241 cgccttatca gaaagtaatt acgcctttaa ttaactcacg attgttaatc acatcgcgct
    15301 cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcggcagtga
    15361 ttttcccttc cgccataagt cgaccgactt tagcgaaatc ttccggcgtg gcgttgcggc
    15421 tgcccatcat cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt
    15481 taaacaggcc gacaaatacc accgtgccac cgtgacgaat cagattcacg gtgttattca
    15541 tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg
    15601 cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag cacgggcaac tccagacgcg
    15661 ttgccacatg ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg
    15721 ctttagcgat tgccgccgcg cccagaccga ttggccccgc tccgaccacc agcacctgct
    15781 cgccgggagc aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca
    15841 atgcggccgc ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca
    15901 aatactcact aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat
    15961 tggtacgccc gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa
    16021 cttgctgacc atttttaaga tcagcaacat ttttacccag cccaacaacc tccccacata
    16081 tttcatggcc taaaacccgt ggataactaa aaaatggttg atttccaccc caggcatgaa
    16141 tatcggtacc gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa
    16201 taggaatctc gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca
    16261 tcgtagacat gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa
    16321 tggcagcgga acggaaaatc tgttttgtga aaacacgcgc ataaaaatgt tttaaatcgg
    16381 gttttaatgg actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt
    16441 gattaaccag gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca
    16501 aagtgcgctg gcggaaatgt acaacatcag ccgcactaca gtgcgtcata ttctcagcca
    16561 cttacgcgaa tgcggcgtct taacgcaggt tggaaacgac tatgttattg cgcgtaaacc
    16621 tgaccatgat gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt
    16681 tgagcaggct tttttcacga tgataaacca gcgccagtta cgcccagggg aaactttttc
    16741 tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg gttgtaagag aatatctttt
    16801 aaaattcggc cgttataatc ttattcaaag cgaaaagcgc ggccagtgga gcatgaagca
    16861 attcgatcaa tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc
    16921 acttcagcac tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat
    16981 gctcgaacgt cacaggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca
    17041 acttgacaga gattttcact ccttactgct ttcagctgcc gacaatatct tttttgaaca
    17101 atcgcttgag atcatatcag tcatctttca ttttcactat caatgggacg aaagcgatct
    17161 taaacagcgc aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg
    17221 ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc
    17281 aatgattcga tccattaatg agaacatgcg ttatgctcat taagtacgga ttaaaaacag
    17341 caaactatta gcgggtgcac ggttttatca aaagataaat accggaaaat tcccgcttac
    17401 ctatgctcac aatcagtcga ctaaaaatgt ttttttgtga gcggtagtaa agtcctaaaa
    17461 ctttaacctg ccgttaatgt caaccttgcg taatgcacaa ttacgtaaac aattaaaatt
    17521 ataaatacag gagtccggcg tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt
    17581 tactccatct tcgtcggcag atattcccgt gccaccagat ggattagttc aacgcagtac
    17641 ccgaattaaa cgcattcaaa ccaccgccat gttgttatta ttttttgcgg cggtaatcaa
    17701 ttatctcgac cgcagttcgc tgtcggtagc aaatttaacg attcgtgaag aattgggatt
    17761 aagtgccacc gaaatcggcg ctttgctctc cgtgttttca ctcgcttacg ggattgcgca
    17821 acttccttgc ggcccactat tggatcgtaa aggcccgcgc ctgatgttgg gactggggat
    17881 gttcttctgg tcactgttcc aggcaatgtc tggcatggtg cacagcttta cgcagttcgt
    17941 gttggtgcgt atcggtatgg gaattggtga agcgccgatg aacccatgcg gtgtaaaagt
    18001 cattaacgac tggttcaaca tcaaagagcg cggacgcccg atgggcttct tcaacgcagc
    18061 ttctaccatt ggcgttgccg taagcccacc gattctggcg gcgatgatgc tggtgatggg
    18121 ctggcgcggg atgtttatta ccattggtgt actggggatt tttctcgcca tcggctggta
    18181 tatgctctat cgcaaccgcg agcacgtaga actgactgcc gttgaacaag cttatctcaa
    18241 tgcaggtagc gtcaatgccc gccgagatcc gctcagtttt gccgaatggc gcagcttgtt
    18301 ccgtaaccgc acaatgtggg gaatgatgct cggattcagt ggcatcaact acactgcgtg
    18361 gctgtatctg gcctggcttc ctggttacct gcaaacagcc tataacctgg atttaaaaag
    18421 cacagggttg atggcggcta tccctttcct gtttggggct gccgggatgc tggtcaacgg
    18481 ttacgttact gactggctgg tcaaaggggg aatggctccg attaaaagcc gtaagatctg
    18541 cattattgcc gggatgttct gttctgccgc ctttacgctg gtcgtaccgc aagcgacaac
    18601 atccatgaca gcggttctgc tgattggtat ggcactgttc tgtattcact ttgccggaac
    18661 atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag
    18721 tatccagaac tttgccagct tcatctgcgc ctcttttgcg ccgatcatta ctggttttat
    18781 tgttgatacc acccactcat tccgtctggc actaatcatc tgcggttgcg tcaccgcagc
    18841 gggggcactg gcgtacatct tcctggttcg tcagccgatc aacgacccac ggaaagatta
    18901 atctccctat gcccgataac attttgctta tcgggcattt ttatggcgat taaaacgttt
    18961 cccagttctc accgctatct gccacggcca ttttacgcgg cgcagctggc atcacggttt
    19021 ttaccacagc cgatgtttca cgctgctgtt gctgctgttg ctgaatccgg aacactgcca
    19081 ctgcttcggt caggcgactg gcctgctctt ccagcgcggc ggcggcggcg gcagattctt
    19141 ccaccagcgc ggcgttctgt tgagttaccc ggtccatctc agcaaccgct aagccaacct
    19201 gatcgatacc acggctctgc tcatcagaag cagaagcaat ttcgcccata atgtccgtca
    19261 cgcgggtcac agcactgaca atctccgcca ttgtttcccc ggcgctttcg accagcgtag
    19321 agccaacatc cactttcccc accgagtctt caatcaggct tttaatttca cgagccgcct
    19381 gggcgctacg ctgggccaga ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt
    19441 gctcacccgc acgcgctgct tcaaccgccg cgttcaaagc cagaatattg gtctggaagg
    19501 caatgccgtc aattacgctg ataatatcgg cgattttctg cgaactggtg gagatatcac
    19561 gcatagtctg caccacgtta tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac
    19621 ttaacgccag gtggctggcc tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca
    19681 gttgctccat gctggctgcc gtctcttcca gcgaagcagc ctgttgctcg gtgcgcgaag
    19741 agagatcgtt attgccggta gcgatttcgc tggcaccgct atagatggca ttggccccgt
    19801 tgcgcacatc accgacggta cgcatcagct ctccctgcat atggcgcaaa ctctctgcca
    19861 gttgccccat ctcattagag ccatccacct caatcggttt caccagatcg ccgcctgcaa
    19921 tatgacgaat gctgtcaatc aggcgattca ttggcgctac cagcgaggct ttaataccga
    19981 accagacggc gaagatgacc gccagtacga cgatcatcac gcccaccaga atccacatcg
    20041 cctggctgta ggaggcattg ttatcgctga cggcgatatc atggagccga tcgttttgtt
    20101 ccatgtaagc cacatactgc ttctcgaaac cgtcctgata tccctgagtc ggctgatcaa
    20161 agaactcgtt gattttgcct gcacctaaca gttggatcag ctccgccagc gcattgtgat
    20221 aaatatcgta attacgtttg atctctgccg ctgcggcggt gctctgacgc gggtcacgcg
    20281 gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt cagcgaaata ctggcactct
    20341 gcatcagctc agcaacggtt gaaccgctac caatattatt ctgatccatc atgtagcgga
    20401 tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc gacccagctg ccattcagcg
    20461 tggattgctg ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg
    20521 cattaaagaa cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca
    20581 gtaagctggt cacaattttg atacgtttta acatggtttc tctttcctgt ggaccgatgc
    20641 aagattttcg gcctggaaag gaaaaacttt atgaatatcc cgcggagatt acgccgagta
    20701 attttattca cactctgaat ttaaaaagcc attatttaca taaattattc acaatataaa
    20761 tatgtgatat gaatcacata tttatcgtca cttaaacgac gcctttgccg ctcaaccgca
    20821 aaactgaccg cttacatccc taaaataacc actcagttat ttaccttact ttacgcgcgc
    20881 gtaactctgg caacatcact acaggatagc ggtcaattta cctcctcaaa cgcaacgcaa
    20941 acctagaacg gcttcggcca actattaatc aatacatgcc aggttttact atggatacta
    21001 aaaagatatt caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg
    21061 cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg
    21121 tatcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag aaggtgatga
    21181 aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc
    21241 cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc
    21301 tggtgggtcc ggttctcgcc gcgcagatgg gctacctgcc tggcacgctg tggctgctgg
    21361 cgggggtcgt gctggccggt gcggttcagg actttatggt gctgtttatc tcctctcgcc
    21421 gtaatggcgc atctcttggt gagatgatca aagaagagat gggaccagta ccggggacta
    21481 tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct cgccgtcctg gcgctgattg
    21541 tggttaaagc cctggccgaa agtccgtggg gtgtcttcac cgtttgctca accgtaccga
    21601 ttgcgctgtt tatgggtatc tacatgcgct ttattcgtcc ggggcgtgtg ggtgaagtct
    21661 ctgtcattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg
    21721 atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg
    21781 gctatgcgtt tgtttccgca ctgctgccgg tgtggctgat cctcgcaccg cgcgactatc
    21841 tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc gctgggtatc gtggtgctga
    21901 acccggaact gaaaatgcct gccatgaccc agtacattga cggtactggc ccgctgtgga
    21961 aaggcgctct gttcccgttc ctgttcatca ccatcgcctg tggtgcggta tctggcttcc
    22021 acgcgctgat ctcttccggt acgacgccaa aactgctggc taatgaaacc gacgcgcgtt
    22081 tcatcggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgctg
    22141 cgtccatcat cgaaccgggt ctttacttcg cgatgaacac cccgcctgct ggccttggca
    22201 tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc
    22261 tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ctggggcttc gtgatttcgc
    22321 cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag
    22381 gtggtgcgcc gacgctggcg gtaggtatcg ctcacgtgtt ccacaaagtg ctgccgatgg
    22441 ctgacatggg cttctggtat cacttcggta ttctgttcga agcactgttc atcctgaccg
    22501 cgctggatgc gggtacccgt tctggccgct ttatgctgca agacctgctg ggtaacttca
    22561 tcccgttcct gaaaaaaacc gactctctgg ttgccggtat catcggtact gcgggctgtg
    22621 tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga
    22681 gcctgtggcc gctgttcggt atttccaacc agatgctggc agccgtagcg ctggtactgg
    22741 gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg
    22801 ctgtatggct gcttatctgc accacctggg cgctgggcct gaaactgttc agcaccaacc
    22861 cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta
    22921 ctgacctgac ggcgcagcag attgccaaca tgaaccacat cgttgtgaac aactacacca
    22981 acgcgggcct gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca
    23041 aaacgtggct tgcggtgcgt aacagcgaca aacgtactga caaagaaaca ccgtacgttc
    23101 caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtgttta gccccgcttc
    23161 ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca ggcaaaaaaa
    23221 tatctcggcc aggcggcgaa gatgttgatt ggcattccag actacgacaa ctacgtcgag
    23281 catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa
    23341 cgccagaatg cgcgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga
    23401 gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcag gcaaaaccac
    23461 cctgctgcgc catattctta acgaacaaca cggctacaag attgccgtga ttgaaaacga
    23521 attcggcgaa gtctccgttg atgatcaact gattggcgac cgcgccacgc agatcaaaac
    23581 gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaagacg cactactcga
    23641 cctgctggat aatctcgaca agggcaatat tcagttcgac cgtctggtca ttgaatgcac
    23701 cggcatggcc gatcccggcc cgattattca gacctttttc tcccatgaga ttttatgcca
    23761 gcgttacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat
    23821 gaaccagttc accatcgccc agtcacaagt gggctacgcc gaccgtattc tgctgactaa
    23881 aaccgacgtc gcaggcgaag cagaaaaact gcgtgaacgc ctggcgcgca tcaacgcccg
    23941 tgcaccggtc tacaccgtca cccacggcga catcgacctg ggcctgctgt tcaacaccaa
    24001 cggttttatg ctggaagaga acgtcatcag caccaaaccg cgtttccact ttatcgcgga
    24061 taaacaaaac gatatttcgt cgattgtggt ggaactggat tacccggtag atatcagcga
    24121 agtttcccgg gtgatggaaa acctgctgct ggaatcggcg gataaactgc tgcgttacaa
    24181 agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg tccagcgcct
    24241 ctacagcgcc gactgggacc gcccgtgggg cgatgaaaaa ccgcatagca cgatggtgtt
    24301 tatcgggatt cagttgccgg aggaagagat tcgggcggcg tttgcggggt tgaggaagta
    24361 attatggatg atggccgcca tgtggcggcc tgaattattt tttgttctga tcgcgcagca
    24421 tcgcttcacg taaaattgcg ttcaggcgag tttgatagcc tttaccaggt cgcttcagcc
    24481 attccatcac atcagcatca atacgcaccg aagcctgggt tttcaatggc cgataaaact
    24541 taccgcgttc tgcgttagac cattgctcat cgctggaagg cgggatatca ctgtaatcaa
    24601 tatcttcgtc gaatttattc gccagtgcct tcagttcggc ctcatgctgc acatcgagtg
    24661 tagagggtgt ccctcgttta tgtttaacca tgctcatagc ggctcctctc tttacgatct
    24721 gcttttcgtg cactgatgat tcgaataatt tcatttccgc tttcgaaacg gatggtatgc
    24781 gcaaccagaa taaccacgat accgtgaacc aggccgatag tctgccagcg atactcaccg
    24841 ttttctatgc gatcttgctg tgacaaatgt tgtggatcat caaacactaa caccgcatct
    24901 tcaaagcgga tgccatgttt cacccgatta cttttggcct tgtttgcatc ccattcaaac
    24961 tccattggca tagttgccat ccctaattgt atatacaaaa atgtatctac aattattctg
    25021 caattatagc gttgatgttg gaaatagcat cacgttttgg ataatcaacc atgaaattga
    25081 caatgagtaa gttggacttg attcatccaa tagaatttct cgcctgacat ggcacactaa
    25141 acacctcgct gtcgttaagg gattaccgtg aaaaactata aagttattac cccgcttttt
    25201 cctacctatg cacaggttaa agctatgatg aaagccgtct caggctattc cctgaaagcg
    25261 gtacgcaata tgattaccgc cattcgcgat caaaccggta cgccgcaaaa acccgttgac
    25321 tggtcggaac ctgacctgtg gatcagcgaa cgcttaacgg gcgaagatgc cgacatcgcc
    25381 cgtcgtattt gggatacaga taatcacata ttgaacccgc gccacagcta cggctgctac
    25441 ttattcctca actatcccca gtttgagctg atggaaagca cgccagatga tacctggcag
    25501 cccacctcgc gcgggcaaaa attcctgcaa gatgacgaaa aaacgctgcg ttcactggat
    25561 gaccaggaag gcattctgca actgcttgag ctgctggctg gccgagaaat gtcgcgccgc
    25621 gccgatttat tgccggaatg gcaggccttt ttgcatcagc attcgaaatt cgcgtctgca
    25681 agttcagtga aaagcacgct ctactcccgt ctctataacc ttattgaccg tggcatggtt
    25741 actcgcgaag gtatgagcta ttgcatcacc gataccggac gcgcctggct tggaaaagcg
    25801 ttgccagcac gaaaaagcga tccgcgtaaa gagctgctgg aagcggtgaa acgctacaac
    25861 gagcagcaaa aagagctgct gcgcgaacaa atcagcacaa tgaacccgta caaatttgaa
    25921 caactggtcg gtcaattact ggaagcaatg ggctacgagc aggtcgaagt gaccaaagcc
    25981 tcaggcgata aaggtgtgga cgtgattggt caggtgcagg tcggcatcac cactattacc
    26041 gaagtggtgc aggtcaaaag aatgcagaac accatcactc gaccgtatat cgaccagttg
    26101 cgcggagcgt tgccttacca caaggccatt cgcggtacgc tcatcaccac cgggaaattt
    26161 gcagcgaaat gtgcagaagc ggcgctcttt ccgggggccg cgcccattac gcttatcgac
    26221 ggcgatcgct tactggaact gttaatcgaa aataacgtcg gcattcgccg cagcaatgca
    26281 gtggaattgc tggatgtgga tttgcagttg tttgatgagc tggagatgga ttaaagggcg
    26341 aaatccgccg cctcaaccat tacaatagcg acccaattta tctgaacaga gttcatcatg
    26401 gccaaagcac caactaaaaa agccaaagca aaaaaaggct ttgaagaaac attatgggat
    26461 accgcaaacc aacttcgcgg tagcgttgag tcctccgaat acaagcacgt ggtgttaagc
    26521 ctcgtgttcc tgaaattcat cagcgataag tttgaagcac gccgcaaaaa aatgattgcc
    26581 gatgggcaag ctgacttcct tgagatggaa gtgttctacc agcaggacaa cattttctac
    26641 ctgccggaag aggcgcgctg gtcgtttatc aaacaaaatg ccaaacagga cgatattgcg
    26701 gttcgtattg acaccgccct ctcgacaatt gagaaacgta acccaaccct gaaaggcgcg
    26761 ctgccagaca actacttcag ccgtcagaat ctggaaacca aaaaactggc gtcactgatt
    26821 gataccatcg acaacatcga aacgctggca cacgaaactg acgttgaaac gttgtcgaaa
    26881 gaagacctgg tcggacgcgt ttacgaatac ttcctcggta agtttgccgc aaccgaaggc
    26941 aaaggcggtg gagagttcta cacgcccaaa tgtgtggtca cgctgttaac tgaaatgctc
    27001 gaacccttcc agggcaaaat ttatgacccg tgctgcggct cggcagggat gttcgtgcag
    27061 tcggtgaagt tcgtcgagag ccatcagggt aaaagccgtg atatcgcgct gtatggtcag
    27121 gaactgacag ccacgacgta taaactggca aaaatgaacc tcgccattcg cggtctttca
    27181 gctaacctcg gcgaacgccc ggcaaacact ttctttagcg accagcaccc ggacctgaaa
    27241 gccgactaca ttctggcgaa cccgccgttc aacctgaaag actggcgcaa tgaagccgaa
    27301 ttaaccgaag atccacgctt tgccggttat cgtatgccgc caaccggtaa cgccaactac
    27361 ggctggattt tgcatatgct ctccaagctg tcggctaacg gcacagcagg ttttgtgctg
    27421 gcaaacggtt cgatgagttc taacaccagc ggtgaaggcg agatccgtgc acagatgatc
    27481 gaaaatgatc tgattgactg catgatcgcc ctacctggac agttgttcta caccacgcag
    27541 atcccggtgt gtttatggtt tatgaccaaa tcgaaggctg ccgacccggc caaaggttat
    27601 cgtgatcgtc agggcgagac gctgtttatt gatgcgcgta acctcggcac catgattagc
    27661 cgcacaacta aagagttaac ggcggaagat attgccacaa tcgccgatac ctaccatgcc
    27721 tggcgcagca cgccggaaga actggctgca cggattgcgc gtggtgacag caagctggaa
    27781 aaatatgaag accaggcagg cttctgcaaa gttgcgaccc tgcaagatat taaagataac
    27841 gactacgttc tgacaccggg ccgctatgtg ggtgcagccg agcaggaaga agacggcgtg
    27901 gcatttgaga ccaaaatgcg tgaattgtcg aagacgttgt ttgagcagat gaagcaggcg
    27961 gaagaactgg atcgtgcgat tcgccagaat ctggaggcgc tgggttatgg ggagtaagtg
    28021 gtgtatatat cgcttaggtg atatagctga attttcatac ggaaaaatgc ctaaaaagga
    28081 gtttgttggt acaggtgact atgtcatttt ttcaggatat aaatacacag aaagctaccc
    28141 tgaaaaaaat acttcggcag gtgagttaat tgttgttgcc cgaggtgttg gtggaactgg
    28201 cgatgttaaa ataacgacta gagattgtta tttaacaaat ttgtccatta aaataaattt
    28261 ggatgaaaca aaagtaagaa aagaatattt ttattatctg tttctacaga gtaatttacg
    28321 atatcttgat agcggttctg ctcaaagcca aataaccatc aatgatctag cgaatgtaga
    28381 aatatctgct ccttcgctaa atgttcaaga tttaatagtc aagagtctca aagcttttga
    28441 tgacaaaatc acaacacttt catccatgaa ccaaaccttg gaacaaatgt cccaaaccct
    28501 gttcaaatcc tggtttgtgg attttgatcc agtgattgat aacgctctgg atgcaggaaa
    28561 tccaatcccg gaagccctgc aaactcgcgc caaattgcgt caaaaagtac gtaatagtgc
    28621 agattttaaa ccgcttccgg cggaaattcg ctcgcttttc ccaagtgaat ttgaagaaac
    28681 ggagttgggt tgggtgccaa aggggtggaa agaagggacg cttccagaga tagcttttat
    28741 aaactccaca tcgtggacta ataaaaatca accagattat atcaattatg tagatctctc
    28801 taatgcgaaa gatggcagaa tatactcaat tgaaaaatta tcatttaatg atgctccaag
    28861 tcgagctcga agaatattaa aaaaagatga tctgattttt ggccttgtca gacctgctaa
    28921 cagatcattt gcatatgtac atgttgatgg tctaacgggt agcacaggct ttgccgttat
    28981 tagagcccaa aagacgatat ataaaaattt catttattac tttgtgacct atgataaaaa
    29041 tatagaagaa ctagcgagaa ttgctgatgg tggagcatat cctgctataa aacctgatga
    29101 tatttgtaca ttacctctaa ttattccatc aataaatata attgagaaat ttgatgaatt
    29161 aacaagcaat ttcagagaga tgatgaataa ctcattaatt caaaacgaat atctcaccaa
    29221 cctccgaggc accttgctcc cgaaactcat ctccggcgag ctatccctgg aagatcttcc
    29281 ggatctcacc accgatacag aagccgcata acgcattttg cccctgtaaa atcaggggct
    29341 ttctggtaag gttttctact gatacgggaa tgcttaccag aaattagcca gggttggagc
    29401 gcgatatgag tctctctttc agtgaagcaa aattagaaca agcgatcatt gaactgttac
    29461 aggatcaggg atatcaacac ctgatcggcg ataatgtccc acgttcgagc ctcgatcagg
    29521 tcattatcga agacgatctc cgtcattatt tagcggcacg ctaccagcct gatggcatta
    29581 ctgaagaaga gattcagcga ctgatcaaac agttcaccac gcttccggcc tccgatcttt
    29641 atgaaagcaa caaaacattt tgtcgctggc tggcaaatgg ttttctgttc aaacgcgacg
    29701 atcggcaaca aaaagatctc tatattgaat tgctcgacac ccggcatcta cctgccgcac
    29761 tgcgccagat atttgacgcc gaagatgtcc cgttgcaaca ggcggcggaa ctcccgcctt
    29821 cctatatgaa tccgccgctt aaccggatta agattgttaa ccagcttaaa atctccggca
    29881 aagataatca gactcgtatt cctgacggca ttctctatat caatggtctg ccattggtcg
    29941 tctttgaatt taaaagtgcg gtgcgcgagc aggaagccag tattggcgat gcctggaagc
    30001 agctctgtaa acgctatcgc cgggatattc cgcaactgtt tatctacaac gcgctctgca
    30061 ttattagcga tggcgtaaat aaccggatgg gcaacctgtt tgcgccctat gaatactttt
    30121 actcatggcg caaagtcacc ggtaatgaaa accgtgaaca ggacggaatt ccatcattgc
    30181 actcaatgat tcaggggctg tttcatccgg tacgtctgct ggatgtgatt aaaaacttta
    30241 tctgcttccc ggataaagcc aagcacgaag taaaaatttg ctgccgatat ccgcagtact
    30301 atgccgcccg caaactctat tacagcatca agcaagcgcg taaacctttc ggtagcggta
    30361 aaggcggcac ttactttggc gcaacgggct gtggtaaaag ttacaccatg caatttttaa
    30421 cgcgtctttt gatgaagagc gtggagtttg ccagcccgac cattgttttg atcaccgacc
    30481 gcaccgatct ggacgatcag ctttctgcgc aaatgtgcaa cgccaaaaat tacattggcg
    30541 acgacaccat cgttcccgtt accagccgtg aagatttgcg taatcaactg gcaggacgca
    30601 atagtggcgg agtctttctg acaacgatcc ataaattcac cgaagacacc gaactccttt
    30661 ctgaacgcaa caatatcatt tgcatctcgg acgaagcaca tcgtagccag gttaacctcg
    30721 accagaaagt catcgttgat aaagaaagcg gaaaagtgcg caaaacgtat ggctttgcga
    30781 aatacctgca cgattcactg ccaaacgcca cctatgttgg ctttaccggc acaccgattg
    30841 acgcgacgct cgatgtcttc ggtgaggtga tcgacagtta caccatgacc gaatccgttc
    30901 aggatgaaat cactgtacgc atcgtgtacg aaggccgtgc ggctaaagtg atcctggact
    30961 ccagcaaact ggaagaagtc gaaaagtatt acgaagagtg cgcaaacgca ggcaccaatg
    31021 agtggcaaat cgacgaaagc aaaaaagcca ccgccaccat gaatgcggtt ctgggtgatg
    31081 aagatcggtt aaaagccctc gcagaagatt ttgccaaaca ttatgaaaaa cgcgtagccg
    31141 aaggttccac cgtaaaaggc aaagccatgt ttgtttgtgc cagccgtgaa attgcctggg
    31201 atttctaccg tcagcttaaa gctattcgcc ctgcctggtt tgaagtgaag caagcacccg
    31261 atggcgtcgt cctgacggaa caggagcaga aagagttacc gccttcagaa atggtgaaga
    31321 tggtcatgac gcgcggtaaa gatgacgacg aggcgcttta tgatttactg ggcacaaaag
    31381 aatatcgcaa agagctggat aagcagttca aaaacgctaa atcgaatttc aaaattgcca
    31441 ttgttgttga tatgtggctg accggtttcg acgtcccgga actggatacc atctatattg
    31501 ataaaccctt acaaaaacat aatcttatcc agactatttc tcgcgttaac cgtaaactgg
    31561 aaggcaaaag taaagggtta gtggtggact acatcggcat taaaagtcag atgaaccagg
    31621 cactggcaat gtattcccgc attgatgcca ccaactttga agatattcag caatcggtaa
    31681 tagaagttaa aaaccatctc gatttgttgg ggcaagtctt ttacgaattt gacagtcggg
    31741 attattttag tggtgagcca caagcgcaat tatcctgcct caaccgcgcg gcggaatttg
    31801 ttctgcgtac ccagaaagtt gaacgtcgtt ttatgggact ggttaaacgc atgaaagccg
    31861 cctacgacgt ctgctgcggc agcgaagcat tatcacagac agaacgtgac tatattcact
    31921 attatcttgc tgttcgttca attgttttca aactgacgaa aggtgacgca ccggatgtta
    31981 cccagatgaa tgcacgcgtt cgtgaaatga ttgcagaagc gttaaaagct gatggcgtag
    32041 aagaaattta ttttcttggc gataaaaaag cggaatccat cgatattttt gacgaagatt
    32101 atctggcgcg aattaacaag atcaaacttc cggcaacgaa gatccagtta ttacaaaaat
    32161 tactggaaaa agcgatcagc gacttcaaga aagtgaacca gttgcaaggg attaacttca
    32221 cccgccgctt ccaggcgatt atagatcgtt ataatgagcg gcgagaagat gatgtactca
    32281 acggtgaaga attcgataca ttcagtcagg aaatgaccga tattatctat gatattaaaa
    32341 ccgaaatggg cacctgggcc gatttaggca ttgatattga agaaaaagcc tttttcgaca
    32401 ttcttgctca tatgcgcgat aaatatcagt tcacctatga cgatgaaaaa atgctgtcac
    32461 tggcaaaaga gatgaaaagc gtggttgaca acacatctaa atatcctgac tggagtaaac
    32521 gcgatgatat taaagcgaaa ctgaaagttg aacttattct gcttctacac aagcataagt
    32581 tcccgccagt agcgaatgat gatgtttata tgggtgtgct ggcacaggca gagaatttta
    32641 aagaacacca tatgagttct ttaaattaat tatgaaggcc ggatacactt tgttatccgg
    32701 ccattctgaa ttaacgttaa tttcccttcc agatctttcc agtaatgctt ttattatttt
    32761 ctatttttag ctggactttt cccatattta ctgatgatat atccaggtat ttagcgcgat
    32821 gcggatgtgt tggcgcacac ccgcaccgct catcacaatc actatttata gggaatagca
    32881 gttatgactg acacgcattc tattgcacta ccgtttgaag cagaagtctc cccggcaaat
    32941 aaccgtcaat taaccgtgag ttatgcgagc cgctacccgg attacagccg tattcccgcc
    33001 atcaccctga aaggtcagtg gctggaaacc gccggttttg ccaccggcac ggcggtagat
    33061 gtcaaagtga tggaaggctg tattgtcctc accgcccaac cacccgccat cgaagagagc
    33121 gaactgatgc agtcactgcg ccaggtgtgc aaactgtcgg tgcgtaaaca aaggcaggtg
    33181 caggagttta ttggggtgat tagtagcaaa acgccacgct aagtttctct ccccttcccc
    33241 acacgggaag gggaattttc cccctcactc cacatcctcc ctgcgtaacc caatatccct
    33301 caaccgctca tcactcatct gctgtaacac tcgtcgggtc tgcatttgcc gccgccaacg
    33361 cctcaccgcc tgccagagtt gcacaagtct gataaacggc gttttggctt tgttttcgta
    33421 aaattccatc tgctgcctcc tcatctttca gaggcttcat catccgtctt ctggcagttc
    33481 acaatacaga tgcaaatatt agttttattt aacatacaga cagattaaaa cagcatctgc
    33541 atccggtttt ttagcctcat ctgtaccggt ttttctctct gtatggtcca aataaggaat
    33601 acagcatgac gcgttatcaa catctggcga ctctacttgc cgagcggatt gagcaggggc
    33661 tgtatcgtca cggggagaaa ttgccgtcgg tgcgcagctt aagtcaggag cacggcgtca
    33721 gcatcagcac cgtgcagcag gcgtaccaga cgctggaaac gatgaagctc atcactccgc
    33781 agccgcgttc ggggtatttt gtcgcacaac gtaaagccca gccgccagtg ccgccgatga
    33841 cgcgtccggt gcagcgcccg gtggaaatta cccagtggga tcaggtactg gatatgctgg
    33901 aggcgcatag cgacagttcc attgttccgt taagcaaaag cacgccggat gtcgaaacgc
    33961 ccagcctgaa accgctgtgg cgggagctaa gccgggtggt gcagcataat ctgcaaaccg
    34021 ttctcggtta tgacttgtta gccggtcagc gagtgttgcg agagcagatt gcccgcctga
    34081 tgctcgacag cggctcggta gtcaccgccg atgacatcat cattaccagc ggctgccata
    34141 actcgatgtc gctggcgtta atggcggtgt gtaaaccggg cgatattgtc gcggtcgaat
    34201 ccccctgtta ttacggttcg atgcagatgc tgcgtggcat gggcgtgaaa gtgattgaaa
    34261 tcccaaccga tccagaaaca ggcatcagcg ttgaagcgct ggaactggcg ctggagcagt
    34321 ggccgattaa aggcatcatt ctggtgccaa actgtaataa tccgctggga tttattatgc
    34381 cggacgcgcg caaacgggcc gttctctctc tcgctcagcg tcatgatatt gtgatttttg
    34441 aagatgatgt ctacggtgaa ctggcaacgg agtatccgcg cccgcggacc attcattcct
    34501 gggatatcga cgggcgagtg ctgctgtgcg gctcgttcag taaaagtatt gcacctggcc
    34561 tgcgcgtggg ctgggtcgca ccggggcgct atcacgataa actgctgcat atgaaatatg
    34621 ccatcagcag ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt gtgctggaag
    34681 gtcactatca tcgccatatc cggcggatgc ggcagatcta tcagcgcaat ctggcacttt
    34741 atacctgctg gatacgggaa tattttccct gcgaaatctg tattacgcgc ccgaaaggcg
    34801 gatttttact gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtc gcgcggcagc
    34861 tgtgccgcat gaaaatccag gtggcggcag gctcgatttt ctcggcttcc ggcaaatacc
    34921 gtaattgtct gcgcaccaac tgcgctttgc cgctcagcga aacctatcgc gaagcactga
    34981 agcaaattgg cgaggccgtg tatcgggcaa tggaataaat tcgacatagt ggcgaagcgc
    35041 ctgatttact ctcacgaata gagtgtgact cgttttatct ttgcagggta atcgccacgt
    35101 tccagcatgg cttaggcaaa gtgcgctttg ttcataccgg atgcgatgtg aacgccttac
    35161 caggtctaca aaatctttaa agttaaatat atttaatggg ttatgtagac ctgataagcg
    35221 tagcgcatca ggctgctttg cgtttgtcat cagtctctga ttagtactcg atccatggat
    35281 cgcgcttgct ttcaggcaag ggctgatcta atgcctgcat ttgctctgcc gaaagtccgg
    35341 tatatcgttg tatccattca ggatccgccc cattctgcaa caacaatcgg aggaggctct
    35401 tttccccttc ttctttccct tctttcctcc ccctctccct tcccaacagc catccatcat
    35461 tatgaattcg ctctgcaatc gtcattattc gctccctgtg ttgtggcatt cgacggataa
    35521 gttcgctgat aaactcatta aaacgcgctt catcgccagt cagtaaaatg taatttaaca
    35581 gcgcggttat ctggctgtca ttagcgcacc ctgtaaccag taatactgcc agttgatcaa
    35641 taagccccat tagatcgcgc tggcgaatat gcttttggat caactccagc agggcgactc
    35701 tgcgatgctg cacaatctcg tcgtctggca cgacagtgac atccaccagc gggaacgctg
    35761 cggtataaag cttccgtgcg gtagccgggt cggcaaattc atccagccag cacaaagacc
    35821 aggggtaagg actacggcta ccgtgataaa acagcatcgg gatgaccagc ggtagcggcc
    35881 ggcgtttatc atgctcgata tggcgctgca tcaccgccat ggaatatcgc atcaggcgaa
    35941 aggccatatg gatatcctcg cggctctgat gttcaatcac cacataaata tagccatctc
    36001 cttcgcgggt ctttaccgac cacagaatat cggaatgtag cgcccgcaat ttttcatcga
    36061 caaagctggc gtattccagt tttaagctgt cgagatcgca cagttcacgt aaatctttgg
    36121 gtaagtggat ctccataaaa tcccgcgcgg tgtcagggtg ggtgagaaaa gatttaaata
    36181 atgcgtcatg cggcgtgctg gtcgtgaagt ttgtcatggc gatccgtcac ctgtagataa
    36241 gatggcccga ctgtagcgac taacaaaacc attctcaccc ggcaatttct cgttccccga
    36301 ggtgctttcc aggctatttt tgttcctctg caaacgatac atttttccgg aaacaggctc
    36361 gcattttaca tttcctgtac ccggcagata ttcccctttc caccgcactc ttttgattaa
    36421 ccgcaaattt taattacact taaggtgtat attttctatg caacccatca attcaagagg
    36481 tgtaatgtgc tgatgactat ttgtaatcgt tatacattct gacctggagt cagaaggtat
    36541 ttctctgtct gtatgttcac aggcagtgtg gttgattaca tgaaattcag tacatttgcc
    36601 cgtctcgcct gcctttcgtc attaccgacg gtattgaatt tcgttttccc cattgggatt
    36661 ctccggacaa ggagttgttt gttatgccac gtttttttgc ccgccatgcc gccacgctgt
    36721 ttttcccgat ggcgttgatt ttgtatgact ttgctgcgta tctgtcgacg gacctgatcc
    36781 agccagggat cattaatgtg gtccgtgatt ttaatgccga tgtcagtctg gccccggctg
    36841 ccgtcagtct ctatctcgct ggaggcatgg cattgcagtg gctgctgggg ccgctttccg
    36901 acaggattgg ccgcaggccg gtgctgatta ccggggcgtt aatttttacc cttgcctgcg
    36961 ccgcgacaat gttcaccacg tcaatgacac aatttcttat cgcgcgtgca attcagggca
    37021 ccagtatctg ttttattgcc accgttggtt atgtcacggt gcaggaggcg ttcggacaga
    37081 caaaagggat caagttgatg gcgattatca cctccatcgt actgattgcg ccgattattg
    37141 gcccgctttc cggcgcagct ctgatgcact ttgtgcactg gaaagtcctt tttgccatca
    37201 ttgcggttat gggttttatc tcatttgtcg gcttgctgct ggcgatgcca gagacggtga
    37261 agcgcggcgc ggttccgttt agcgccaaaa gcgtcttgcg cgattttcgc aatgtctttt
    37321 gcaaccggct gttcctcttt ggcgcggcaa ccatcgccct cagctatatc ccaatgatga
    37381 gctgggtggc tgtctcgccg gtgatcctga tcgatgcggg cggcttaaca acttcgcagt
    37441 tcgcctggac gcaggttccg gtattcggcg cggtgattgt cgccaacacc atcgtggcgc
    37501 gatttatcaa agatccgacc gaaccgcggt ttatctggcg tgccgtgccc attcaactgg
    37561 ccggcctcgc actgttgatt gtcggcaatt tgctgtcgcc acacgtctgg cagtggtcgg
    37621 tgctcggcac cagtctgtat gctttcggga ttggtttaat cttcccgacc ttattccgct
    37681 ttacgctgtt ttccaataac ttaccgaaag ggaccgtctc cgcatcactg aatatggtga
    37741 tcctgatggt gatgtctgtc tcggtcgaaa tcggccgctg gctgtggttt aacggcggtc
    37801 gcttgccgtt tcatctgtta gccgtcgtag cgggcgttat cgtcgttttc accctggcgg
    37861 gattgctcaa tcgcgtgcgc cagcatcagg ctgccgagct ggcggaggag cggtgatttt
    37921 tgcgcgatcc tgccgtcagg ctctattctt aacgttatga ataaactcat tgaactcaga
    37981 cgcgccaaaa tgttggcgct ctctttactg cttatcgccg ctgctacctt tgtcgttacg
    38041 ctgtttttgc cacccaattt ttgggtgagc ggcgtgaagg cgattgctga agcggcgatg
    38101 gtcggcgcgc tggcggactg gtttgcggtg gtggcgctgt ttcgccgcgt gccgattccg
    38161 atcatttctc gtcatacggc gattatcccg cgtaataaag accggattgg cgaaaatctc
    38221 ggtcagttcg tgcaggaaaa atttctcgat acccaatcgc tggtggcatt gattcgacgt
    38281 cacgaaccgg cgttgttgat tggcaactgg tttagtcagc cagaaaacgc ccgccgcgtt
    38341 ggtcagcatc tgttgcagat catgagtggt tttcttgaac tgaccgatga tgcgcgtatt
    38401 cagcgcctgc ttaagcgcgc agtccatcgg gcgattgata aggtcgatct ttccggcacc
    38461 agtgcgttga tgctggagag tatgaccaaa aacgatcgtc atcaggtgct gctggatacg
    38521 ctgatcgcac agttgatcgc ccttctccag cgcgataaat cgcgcaagtt tatcgcccag
    38581 cagattgttc gctggctgga gagtgagcat ccactgaaag ccaaaatttt gcccactgaa
    38641 tggctgggcg aacatagcgc ggagttggtt tctgacgcgg tgaattcttt gcttgatgat
    38701 attagtcgcg atcgtgcgca tcagatccgc catgcgtttg atcgcgccac ctttgccctg
    38761 atcgacaagc tgaaaaacga tcctgaaatg gcagcgcgaa ccgatgccgt aaaaagctat
    38821 ctgaaagaag atgaagcttt taaccgctat ctcagtgaat tgtgggggga tttacgggaa
    38881 tggctgaaag cggatatcaa cagtgaagat tctcgtgtga aagaacgcat cgcacgagcg
    38941 ggtcaatggt ttggcgaaac gttaattgcc gatgatgcct tgcgggcgtc gttaaatggt
    39001 catcttgaac aagccgcgca ccacgtcgcg cctgagtttt ccgcattcct gacgcgccac
    39061 atcagcgata cggtaaaaag ctgggatgcg cgggatatgt cgcggcaaat agaattaaat
    39121 atcggcaaag atctgcagtt tattcgtgtt aacggtacgc tggttggcgg atgtattggg
    39181 ctgattttgt atttgctgtc gcagctcccg gctttgttcc ccctcggcaa tttttagaaa
    39241 tccatgaaaa actaagtggc taaccgcgag agagatcaaa taaaccgcac taaaagaatg
    39301 gtaataatgt ggcttctttt cattatctcc cgtggtacgg ggaaggaaaa tcatgtcact
    39361 tgtcaccgat ctacccgcca ttttcgatca gttctctgaa gctcgccaga aaggctttct
    39421 caccgtcatg gatctcaagg agcgcggcat tccgctggtt ggtacctact gtacttttat
    39481 gccgcaagag atcccaatgg cagccggtgc ggttgtggtt tcactctgtt ccacctctga
    39541 tgaaaccatt gaagaagcgg aaaaagacct gccgcgcaat ctctgcccgc tgattaaaag
    39601 cagctacggc ttcggcaaaa ccgataaatg cccctacttc tacttttctg atctggtggt
    39661 cggtgaaacc acctgcgacg gcaaaaagaa aatgtatgaa tacatggcgg aatttaagcc
    39721 cgttcatgtg atgcaactgc caaacagcgt taaggacgat gcctcacgcg cgttatggaa
    39781 agccgagatg ctgcgcttgc ataaaacggt agaagaacgt tttgggcacg agattagcga
    39841 agatgctcta cgcgatgcca ttgtgctgaa aaaccgcgaa cgtcgcgcac tggccaactt
    39901 ttatcatttg gggcagttaa atcctccggc gcttagcggc agcgacattc tgaaagtagt
    39961 ctacggcgca accttccggt tcgataaaga ggcgttgatc aatgaactgg atgcaatgac
    40021 cgcccgcgtt cgtcagcagt gggaagaagg ccagcgactg gacccgcgtc cgcgcatttt
    40081 aatcaccggc tgcccaattg gcggcgcagc agagaaagtg gtgcgcgcaa ttgaagagaa
    40141 tggcggctgg gttgtcggtt atgaaaactg caccggggcg aaagcgaccg agcaatgcgt
    40201 ggcagaaacg ggcgatgtct acgacgcgct ggcggataaa tatctggcga ttggctgctc
    40261 ctgtgtttcg ccgaacgatc agcgcctgca aatgctcagc cagatggttg aagaatatca
    40321 ggtcgatggc gtagttgatg tgattttgca ggcgtgccat acctacgcgg tggaatcgct
    40381 ggcgattaaa cgtcatgtgc gtcatcagca caacattcct tatatcgcta ttgaaacaga
    40441 ctactccacc tcagatatcg ggcagctcag cacccgtgtc gcggccttta ttgagatgct
    40501 gtaaggagtg gcagtggcat attcgattgg cattgattcc ggctcaaccg ccaccaaagg
    40561 gatcttactg gcagacggcg tgattacgcg ccgtttcctc gttccaacac cctttcgccc
    40621 ggcaacagca attactgaag cctgggaaac tctgcgcgaa gggttagaaa caacgccgtt
    40681 tctgacgctc accggctacg gacggcaact ggtggatttt gccgataaac aggtgacgga
    40741 aatctcctgt cacgggctgg gcgcacggtt tcttgcgcca gcaacgcgcg cagtaatcga
    40801 catcggtggt caggacagca aagtgattca gcttgatgat gacggtaacc tgtgcgattt
    40861 cctgatgaat gacaaatgcg cggcgggcac cgggcgtttc ctggaggtga tctcgcgcac
    40921 gcttggcacc agcgtcgagc aactcgacag cattaccgaa aatgtcatac cgcacgccat
    40981 cacgagtatg tgcacagtgt ttgctgaatc agaagtgatc agcctgcgct ctgcgggcgt
    41041 agcgccagaa gcgattctcg ccggagtgat taacgcgatg gcgcggcgga gcgccaattt
    41101 cattgctcgt ctctcctgtg aagcgccgat tctgtttact ggtggcgtta gccactgtca
    41161 aaggtttacc cacatgctgg aaagccatct gggaatgccg gtacaaactc accctgatgc
    41221 gcaattcgct ggtgcaattg gcgcggcggt gatcggtcag cggcagagaa aacgcgcatg
    41281 aaagagtatc tgtttttatt tcactccacg gtcggcgtca tacaaacccg caaggcgttg
    41341 caggcagcgg gcatgacttt tcgcgtcagc gatattcccc gtgatttacg cggcggctgc
    41401 gggttatgca tttggctgac ctgtccgcct ggcgaggaaa ttcaatgggt gatccctggg
    41461 catacggaat cagtttattg tcagcaggat ggtggctggc gctgcattgc acattacggg
    41521 atttctccgc gttaaggatt cattaagatc gctacgtata cttctcgata tcttattgat
    41581 ttgccttaaa gggattaccg tgacaaaaag tatttcgtta agtaaacgca tcttcgttat
    41641 tgtgattctt tttgtcattg tggcagtgtg tacattcttc gtgcaatctt gcgcccgtaa
    41701 aagcaatcat gcagcatcat ttcagaatta ccacgccacc attgacggca aagaaattgc
    41761 aggcataacg aataatatct cttcgctgac ctggtcggca caaagcaata ctctttttag
    41821 cactatcaac aaacctgccg ccattgttga aatgaccacc aatggcgatt tgatccgcac
    41881 aattcctctg gattttgtta aggatctcga gacgatcgaa tacattggcg ataatcagtt
    41941 tgtcattagc gatgagcgcg attatgccat ttatgtgatt tcactgaccc caaactcgga
    42001 agtaaaaatc ctcaaaaaaa taaaaatccc gctgcaggaa tccccaacca attgcggttt
    42061 tgaaggattg gcctattctc gccaggatca cactttctgg ttttttaaag agaaaaaccc
    42121 gattgaggtc tacaaagtta acgggctgtt aagtagcaat gaattgcaca tcagcaaaga
    42181 caaggcacta caacggcaat ttactttaga tgatgtgtcc ggcgcagaat ttaaccaaca
    42241 aaaaaatacg cttctggtgc tgtctcatga atctcgcgca ctccaggaag taacgctggc
    42301 tggcgaggtt ataggtgaaa tgtcactaac caaaggcagt cgaggtttgt cgcataacat
    42361 caaacaggcc gaaggggttg cgatggatgc ctcaggtaat atttatatcg ttagcgaacc
    42421 aaatcgtttt tatcgcttta cccctcaatc atcgcattaa caggctggca tgtttcacct
    42481 ttttgttatt aacttgttaa caacaaaaat gacaaacaag gttaaacatg ccttcgccct
    42541 cgcatcccgt agaacgcttt tctttcagca ccgcgctttt cgggatgctg gttctgacct
    42601 taggtatggg tttaggccgc ttcctttata cgcctatgtt gcccgtcatg atggcggaag
    42661 gatcgttttc atttagccag ctctcgtgga ttgccagcgg caactatgcg gggtatctgg
    42721 ctggcagtct gctattttcg tttggcgcat ttcaccagcc atcgcgcctg cgcccatttc
    42781 tgttggcttc tgccctggcg agcgggttgt tgatcctcgc aatggcatgg ttgctgccat
    42841 ttattctggt gttattgatt cgcgtcctgg cgggtgtcgc cagcgccggt atgctgattt
    42901 ttggttcgat gctgattatg cagcacacgc gccatccttt tgtgctggca gctttgtttt
    42961 ctggcgttgg cattggcatc gcactgggca atgaatatgt tctggcaggc ctgcattttg
    43021 acctctcctc gcaaacgtta tggcaaggcg ccggggcgct ttctggcatg atgctgattg
    43081 cacttacgct tttaatgccc tcgaaaaaac acgccatcac accaatgcca ttggcaaaaa
    43141 cggagcaaca gataatgagc tggtggttac tggctattct gtacggcctg gcgggatttg
    43201 gttatatcat cgtcgcgact tacctgccgc tcatggcgaa agatgcaggc tcaccattgt
    43261 taaccgccca tctctggacg ttagtcggct tatcaattgt gcctggttgc tttggctggc
    43321 tatgggcagc aaaacgttgg ggggctttgc cctgcctgac ggcgaatttg ctggtgcagg
    43381 ctatctgtgt gctgcttact ctcgccagcg actcgcctct cttgcttatc atcagcagtc
    43441 ttggttttgg cggtaccttt atgggcacga catctctggt gatgactatc gcccgccaac
    43501 tgagcgtacc gggaaatctc aatcttttag gctttgtgac gctcatttac ggcattggac
    43561 aaatcctcgg cccggcgctg accagtatgc tcggcaatgg aacatcggcg cttgccggcg
    43621 ccactctctg cggcgcggcg gcgttgttta ttgctgcgct tatcagcaca gtacaattat
    43681 ttaagctgca agtggtcact tcatgaggag tgtttccctt accactcaag aaattcgacc
    43741 gcaaccgttg atgtcagcca gaccccgttt tccgcttgcc agaaagggag gcctcgtttc
    43801 gccatttcct gtgctttgac ggttaaaata accggcgatc cgtgtcttgc tcccactttg
    43861 cgtgccgttg cttcatcagc agagagatgt acgtagtgac gctcacctgc aatcagcccc
    43921 tgttttttta tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt
    43981 gttttttcgg caaaggaaat agcgacctgc gaagtcgaat gcccctgaac cgcgcggatg
    44041 cacctaccat cactggaata actaaaacgc tttttatcgc tggtggcaac cacggtatcc
    44101 agcaatgcac gggttaaccg cttgcccact ttttgggcac aaagtatgag cttatcgata
    44161 tccgcccatc cttcacggtc cagtacgata ccaatcgcct ccggtttgtg tcggaggaca
    44221 aaacttaaaa acttgctcgt atccgccagc tctttttcgt tgtactttgc catcgcgttt
    44281 tcgtccctgg attatctgtt ttttatttac tatgtcattg tcactgggtt tatgaatttt
    44341 ctgcaactca atcctgcgta gaaccttttc cacacagcgt aattttaccg ctatgcaaaa
    44401 aacgcatagt cagaaacata cttgcatttt ccccataccc acatggctga taaggttttt
    44461 cagctccagg aattttcctg gctcaagcaa cacacaaaca caaaaaaaca atggggattg
    44521 ttatgacaca acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta
    44581 aagggtatct ggcttttttt ctcaccataa tctttttttc cggtgttttt tccgggacag
    44641 acagttggtg gcgcgttttt gatttttccg ttctgaacgg atcgtttggg caactgcctg
    44701 gtgcgaacgg agccaccacc tcgtttcgcg gcgcgggtgg cgcgggggct aaagatggct
    44761 ttctctttgc gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta
    44821 ccgatggtct tggcggatta cgcgccgcgc agcagttaat gacgccagtg ctaaaaccgt
    44881 tgttgggcat tccagggatc tgctcgctgg cgctgatcgc taacctgcaa aacaccgatg
    44941 ccgccgcagg tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag
    45001 tgattttcgc cgcatatcaa accagcggta gcgccattat caccaactac ttctcttccg
    45061 gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt acctttagca gtcatcctgg
    45121 tgtttaaatt tgtcggagcc aacattttgc gcgtctggct taacttcgaa gaacgtcgca
    45181 acccaacgca aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt
    45241 tatcgatggt gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt
    45301 gatggcgttt gtcatcattc aggcgctgaa aattaccggc ctgctcgact gggtggggca
    45361 tatctgtgaa cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt
    45421 agccgcatta atgagtatgg gtggcgcagt cggtgttgcc gccagtctgg caaccgctgg
    45481 cgcattaacc ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa tgggcaaccc
    45541 ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca
    45601 tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt
    45661 ttaacaagga gttaccatga ttgattatac cgcagccggt tttaccctgc tgcagggagc
    45721 gcatttgtat gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat
    45781 tatcgccgtt gccagtaata tcccttctga cattgtaccg gactgcacgg ttgtcgatct
    45841 cagcgggcag atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg
    45901 cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg ttaagtcgtc tgacggaagc
    45961 aggcgtcacg tcagtggttg gtctgctggg caccgactct atctctcgcc acccggaatc
    46021 cctgctcgcc aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg
    46081 cgcttatcat gtcccttccc gcactattac gggttccgtg gaaaaagacg tggcgattat
    46141 cgatcgcgtg attggcgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt
    46201 ttatcacctg gctaatatgg cggcggaatc ccgcgttggc ggtttgctcg gcggtaaacc
    46261 tggcgtcacc gtgttccaca tgggcgacag caaaaaggcg ttacagcctg tctatgacct
    46321 gctggaaaac tgcgatgtgc cgattagcaa gttgctgccg acccacgtta accgcaacgt
    46381 tccgttgttt gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag
    46441 cagcattgac gaaccagtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat
    46501 tccgctggca cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgatga
    46561 cgaagggaat ttaacccata tcggtgttgc cggttttgaa acgttgctgg aaaccgtgca
    46621 ggtgctggtc aaagattatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag
    46681 cgtagccggt ttccttaacc tgagcgggaa aggcgaaatt ctgccaggca atgacgcaga
    46741 cttactggtc atgacgccag aattacgcat tgagcaggta tacgctcgcg gcaagctgat
    46801 ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa acggcataaa cggttgtcag
    46861 ccgcccgaat ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac gtaatggatg
    46921 actgtggtgc ggttttgcat aatattgaaa ctaaatggct ttatgatttt ctgaccctgg
    46981 aaaaatgccg caatttttcc caggcagcag tcagtcgcaa cgtctcgcaa ccggcattca
    47041 gccgccgcat ccgtgcgctg gaacaggcga ttggcgttga attgtttaac cgccaggtga
    47101 cgccgctgca actctcggaa caaggtaaaa tattccattc gcagatccgc catctgttgc
    47161 aacagttaga gagcaacctt gcagaactcc gtggtggcag cgattacgcg caacgcaaaa
    47221 ttaaaatagc cgctgcacac tctctttccc tcgggctgtt accgtccatt atcagccaga
    47281 tgccgccgct ctttacctgg gcaattgaag ctattgatgt cgatgaagcg gtcgataaac
    47341 tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc
    47401 cgtttgacca cattcgctta tttgaatctc aattgttccc cgtttgcgcc agtgacgaac
    47461 acggagaagc actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca
    47521 actcctacat ggggcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca
    47581 gcaccttttt tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg
    47641 ggattgcctg gctaccggaa tacgccatcc aacaagaaat tcgcagcggg cagctcgttg
    47701 tactgaatcg ggacgaactg gtgatcccga ttcaggccta tgcataccgg atgaataccc
    47761 gcatgaatcc agttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta
    47821 gctgacattc tccagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt
    47881 tagcgattca tcgtcgtgat acagcattat ctcaaggctg atcgccagag tgtggcagta
    47941 cgcttctttc aaattaacct gtaacgccgc aatccgtggc tcatgaaata acagtgcaga
    48001 acgcagctga cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca
    48061 ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt
    48121 gaggagatcg ctgcatactg atttacgcag cggtgacgtt ttattgcctg aaatttcagg
    48181 taaaacagcc tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt agcaacatat
    48241 gcggcttgcc ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc
    48301 ttttgttcat tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa
    48361 aaccttactt gcctatgaat atctactgac ttttcaggaa aaaagtcatg atttgataaa
    48421 aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa tattccgaat agttacgtag
    48481 atgaaaatac ttgtcaccac cgctacttaa ttccggtggg aaattattta tttccgcata
    48541 atcagctatg tattatttaa aacaattagt ttgtaaataa tataaattga taacatatca
    48601 aatacatcaa aaataccatt cacaggcatt attaatatag acatcaatat agcaacatca
    48661 aattccaatt agtaataaga tatgctttta aattgcatgc aaataatttt aaatgagcgt
    48721 tttttgatac tttagagtgt ttcccaccat ttaatatcac tgtgtagagt tgatatcact
    48781 acaacagaaa catgcatgtc atttgtttac ttctcttttt actacagaaa aatttatcta
    48841 tttttctaag catcagtgaa atactcataa aggtaactat tatgtctatg ccattaagca
    48901 atgcccttca gagttaaatt attacagata atcacttcct tcatcatcca aaagtcgaca
    48961 gtgagttaac acgtaaacat gaacgtgcac gcatggatac ggaaaatatc tatcttccgc
    49021 ctcttgctcg tggcaataac cataattacg atggaaaaag cgttgtcgaa attagaaaac
    49081 ttgatattag taaagagtcc tggccattta attatgtgac agaaacatgc agagaattca
    49141 atggtattac aacaacagga cgtatgctct atcgaaactt gaaaatcacc tcagctttag
    49201 acgaaatata tggtggtata tgtaaaaagg cgcatgctgc cacagagtta gcaaaaggat
    49261 tacggctaaa tttatttatg aaaggccctt tcgatcctgt tgaggactat acagttcatg
    49321 aaataacatt aggccctggt tgcaatgttc ctggttatgc cggaaccacg ataggatata
    49381 tttcaaccct tccagcttct caggcaaaaa gatggaccaa tgagcagcca aggattgata
    49441 tctatattga tcagataatg acggtaacag gggtagcaaa ttcttccgga ttcgcccttg
    49501 ccgcattact taatgctaat attgagttgg gtaatgaccc aataattggt atagaagcct
    49561 atcccgggtg atgaaaatgc accactcaaa cgtatgactc tgcaaccgtc atctcttcca
    49621 gaactgtttg aattaaaaaa tggtgaatgg aattatattg gtaaataact accaggaaga
    49681 atgagtacta acattcaggt tatacagata gctaaacaat cccctgcaat ttacaaagta
    49741 atactcattg cgacttctta atttaagaaa gcgcaaggaa cgtttaccct tgcgcttatt
    49801 ataataagtc aggcgtcgac tttatccagc ggccatgaat caaacagata gccatcaaaa
    49861 taaatatcgt caacgttcga gaattccagc agacgttgct taacgttttc cagatgctgc
    49921 cacattgcca gcttcgccgc tcgggcatct tttttgatta acgcggcgag gatctgtttg
    49981 tgatcgccca accactcttt gcgatacagg ctgtcatcca gatggctgtg caactggatc
    50041 cacattggat tgttttcccg ccactgccag gactgacgga acagctccac cagcatgctg
    50101 ttatgcgttg cttcggcaat agcgagatgg aactgcatgt caccgctttc gctgctgccc
    50161 ggcgcactgg aagccagttc acgctcttcc agttgcaatg cctgacgcat tttgacgata
    50221 tcttcgcggg tagcctgcag ggcggcaaac tcggcgatgt tgctctcaag aagctgtcgc
    50281 gcctgcaaca gctcaaaagg acccgcatca ttgcaaacgt tggcatccgg actgtctgtg
    50341 ttgtggctgc ctgagctatc aagaacatag ataccggcac cccggcgcac ttccaccagc
    50401 cctttgatct ccagcatgat cagcgcttca cgtaccaccg tccgcgtgac atcaagcatt
    50461 tctgcaattt cacgctccgg cggcaaccgt tcgccaggat tgtacggcgt ctttatgatc
    50521 agatcgcgga tcatcgcccc gacttcctgg taaggtcttt gcgcagaggt ggcagatttc
    50581 atggtgttgt gtccgttagg tgcggttaat caaatttttg ccggatgcgg cgtgaacgcc
    50641 ttatccggcc tacaacgggc aattttacgg cacccgttaa tctcaaacta taccattagc
    50701 actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa ctggtcaacc aaatatcagt
    50761 ttgattgacc agtaagtctg tagggtaatc agttagttac agcgcagcca cacactcgcg
    50821 cgcaccgcgt tcgcacagct gctgatatgc cgccgtcacc gcgccaacaa agtcggcatt
    50881 ctgcggcaga tcatcggcaa aaataccgct caggcccagc agcgctttca cgcggtctgc
    50941 gccctgatac tgcgcgttaa tcttctggaa ctcggccagc atcgggtcga ccacgtcaat
    51001 ggcattaccc tgctcatcca cgccctgggt gtaacgcatc cagccagcca cgcccagcgc
    51061 caggtgacgc cagctgccgc cgttttgcag gtgcagacgc accggatcca gcagacgctg
    51121 cggtaacttc tggctgccgt ccatcgcaat ctgccaggta cggtgacgca gagacgggtt
    51181 gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg tccgtacctt ccggcatcga
    51241 cagcgttggc gcttgttcct gcatcatcag ggcaaaggcc gctttgcgat aatccgggtt
    51301 agtcatggtg tcggcaatag tttcatagcc gccgaggtaa ccgaggtatg ccaggaaaga
    51361 gtggctgccg ttcagcatac gcagcttcat catttcgaac ggcacaacgt ctgcaacgaa
    51421 ctgtgcgcct actttatccc agtccggacg accattaacg aagttatctt caatcaccca
    51481 ctgacggaac ggttcgcagg caatggcgca cgggtcataa acacccagtt ggtcagcaat
    51541 ttcctgtaag gtttctggcg tcgccgccgg aacgatgcgg tcaaccatgg tgcacgggaa
    51601 ggtgacgttc tcttcaatcc atgccgccag ctgcgggtca cgcgcctgag ccagccccag
    51661 taccgcgacc ttcgccacat gaccgttttc acgcacgtta tcgcaggaca tcaccgtaaa
    51721 cgctttcagc cctttttcac gacgcagacg cagggcttcg acgatgtaac caatcgcgga
    51781 cttcggcgca gtcgggtttt ccagatcgtg cttgatcagc gggttattga gatccagttg
    51841 accgcttgcc gcatcagcgc agtagccttt ttccgtgacc gtcagagaga caatcgccgt
    51901 ttgcggacgc gccatcgcgt tgagaatacc ttcgcagccg tcgatttccg ggtgcagcgc
    51961 ttctttcatc gaaccgataa ttttcagctc ggtgctttcc gcgccttttt ccgccacggt
    52021 gtacagcagt tgctgttttt tcaggttttc gatcagcacg cggtcgttgc ctggcatcag
    52081 gttaacttcg cagatacccc agtcgctgtc ggtgctttcc agcagatgat gggtgtacag
    52141 cgcctggtgc gcacggtgaa acgccccgca accgagatgc acaatgcgtg attccagacg
    52201 agaatgatcc catgacgggc gggcaaccgg cagattgctg tcaacaatag tagtcatcgt
    52261 aaactccaga atccgtcttc agagagtgac ggcaaggaag aaggaaccgg cacggcgcgc
    52321 agccatgccg gtggatatta acggctaaag aaagcgcgct ggatcgccag ttcgacaccg
    52381 cgaacttcgg ccaggccttt cagacgacca attgcggagt aacctgggtt ggttttcttc
    52441 ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca tcgggatcag gtcttctttg
    52501 ccttccgctt tacgacggtg ttcttcttca acaatcgctt tcaccacttc gtacatatca
    52561 acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg ggttatcttc acgcatggtg
    52621 gagcgcagat gggtgaagta aatacgcgga ccgaactgct tgatcatatc aaccagatcg
    52681 ttgtcagcac gcacgccgta ggaaccggtg cacatggtga aaccgttcgc catgctattt
    52741 acggtatcaa ccatccactg catatcttca atggtagaaa caatgcgcgg caggccgagg
    52801 attgggcgcg gcggatcgtc cgggtgaaca gccatacgca cgccaacttc ttcggcaacc
    52861 ggaataatcg ccttcaggaa gacggcaaag ttttcgcgca gtttggcttt gtcgatatct
    52921 ttgtacagct ccaggtgctt acggaactgg tcgagggtgt acccttcttc cgcgcccggc
    52981 agaccggcaa tgatgttacg ggtcagacgc gctttgtctt cgtcgctcat agtggcgaag
    53041 cgttcagctg cctgagcaat ttcttcttcg gtgtaatccg cttccgcacc tgggcgtttc
    53101 aggatatgca tttcgaatgc agcgaattcg atctggtcga agcgcagagc tttggagccg
    53161 tctggcagca cgtattcgag gtcagtacgg gtccagtcga gcaccggcat gaagttgtag
    53221 cacacggtgc gaataccgca ctgcgccagg ttgcgcagag tctgctgata gttagcgatc
    53281 cactgctcat agttgccagt gtgggttttg atatcttcgt gaattggcac gctttctacg
    53341 acagaccaca ccaggcctgc atcttcaacg atagccttgc gtttgaggat ctcttcaacg
    53401 gaccatactt cgccgttcgg gatatggtgc agcgcggtaa ccacgccagt tgcgcccgcc
    53461 tgacggacat cagctaaaga aaccggatcg tttgggccgt accagcgcca ggtctgttcc
    53521 atgatttttc ctctttcaca tagtgggttc cacagcaaag agacgcggaa ccctggagtt
    53581 gtttaggtat ccggatgaac aaccagaccg cagccgtggc gttgcatcgg atgagatggc
    53641 gtataagttc taccttttgg taatacatgt caacccgaat tctaaaattg gttaaccaca
    53701 tcacaagaat ttcactctcc tggttgttgc tgcaaattgc agcaaagggt aatcgatggt
    53761 tattgatcag aggatgtgac attcatcgca acaatggttg accaatttac ataacatgtc
    53821 gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt tatgcatgtg cttaacattc
    53881 tctgggtggt attcggcatt ggtctgatgc tggtactgaa tttgaagttc aaaatcaatt
    53941 caatggtggc tttgttggtg gcggcgctgt ccgtcgggat gctggcgggc atggatttga
    54001 tgtcgctgct gcacaccatg aaagcgggct tcggcaacac gctgggggaa ctggctatca
    54061 tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga ctccggcgcg gctcaccaga
    54121 tagcacatac gctgctggcg cgtctcggtc tgcgctatgt acagctgtcg gtgattatca
    54181 tcggcctgat ttttggtctg gcgatgttct atgaagtggc ctttatcatg ttagcgccgc
    54241 tggttattgt cattgccgcc gaagctaaaa ttccgttcct gaaactggcg atcccggcag
    54301 tagcagctgc cactaccgca cattcactgt tcccaccgca gccgggtcct gtggcgctgg
    54361 tgaatgctta tggcgcagat atggggatgg tttatatcta tggcgtactg gtgacgatcc
    54421 caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct cggcaatctt gagcgcccaa
    54481 cgccatcatt cctgaaagca gatcaaccgg tagatatgaa caatctgccc tctttcggcg
    54541 tttcgattct ggtgccgctg atcccggcga tcattatgat ctctaccacc atcgccaata
    54601 tctggctggt aaaagatacc cctgcctggg aagtggttaa ctttatcggt tcctcgccga
    54661 ttgcaatgtt tattgcgatg gtggttgcat tcgtactctt tggcaccgca cgtggtcatg
    54721 acatgcagtg ggtgatgaac gcttttgaaa gtgcggtgaa aagcattgca atggtgattc
    54781 tgatcatcgg tgcgggtggc gtgttgaagc aaactatcat cgacaccggc attggcgaca
    54841 ccatcggcat gttgatgtcc cacggcaata tctcgcccta cattatggca tggctgatca
    54901 ctgtgctaat tcgtctggcg acgggtcagg gtgtcgtttc ggcgatgacc gccgccggga
    54961 ttatcagtgc tgcaatcctt gatccagcaa ctggtcagct ggttggcgtg aatccggcgc
    55021 tgctggtact ggcgacggct gcgggttcca acaccctcac ccacattaac gatgcatctt
    55081 tctggttgtt caaaggttac tttgacctgt cagtaaaaga cacgttgaaa acctggggtc
    55141 tgctggagct ggtcaactcc gtggttgggc tgattattgt gttgattatc agcatggtag
    55201 cgtaaaagag cacctgagcc tgccattggc aggctctgtt cggattgtcg gcaaagtgca
    55261 ctttgtttat gccagatgcg acgctggcgc gtcttatctg gcctacaaag ggctaacgtg
    55321 cagtttttta gcttcaggca atattgcgta cctgcattag caatgttctg tgatttcttt
    55381 attgataaac aaaagtcacg ccaataatcg attgcacatt ccctgcagtc acctgccctc
    55441 cggtacgtgc gtaatttgcc gttaatccca gacttaccgc cgaagtccct actgctccta
    55501 acgataccgt gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc
    55561 cctgcgctgg tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg
    55621 tgccggagag gtaatacccc aggttttggc ttttcgcaca ataaacggta agaggaattg
    55681 gcactgaacc agggtagtcc ggcagagtaa cggtgacatc atgagcagaa acatcgcagc
    55741 cgccagtagg caccaccaca tcattattgg cgtaaatatt ccacacaaac tggaaatcat
    55801 cgctgttata gttgttggtc tgtcgcaaaa taagcacggc aattaatgag ccagctttaa
    55861 tcgccacccc acccgcactg ctcacaggcg tcaaataaag cgccaccggc cacggcttat
    55921 ccgttctcga attataaaca acccgcggcg tttcgctggt agtcggaaat ggatagctac
    55981 tgccactata ttttacggtc ccggaaaaat tagataacac gccgccataa gccgagcctc
    56041 gttgcagtgt gacatagtct gtaatggttt ccggataatc gttatggcaa aagatttgcg
    56101 tcgaaagatc tacgaccagg ttttgcccca cattcacggc aggcgcaagg tttacataaa
    56161 cattagcgct gccaccgcca ataggaatag cggtaccatt ggcggtttta caggcgaatg
    56221 accaggcatt taccgaccag cccatcagca gtacagcaaa cagggtaata actcgtttca
    56281 tgacaattat ctcttcgggt tcagctgtag gtataggtga tgctaatcac tgcctgaatg
    56341 gttccctgag tggctccgcc atttaccgtc aatgctctga cctgtaacgg gaagtgcgct
    56401 gattgtgagg aatcatccac ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca
    56461 ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg
    56521 gtactgtcgg ctgccccgct gaagctggca gtgaccctcg acgttcccac cggacaatta
    56581 gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc cggcagacat cagactgaaa
    56641 gaataaagat cgccgagatc aaacgtggca ttggtggtgg aaaccgtgca cggtttggcg
    56701 acgaccttac cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc
    56761 gccaatattg ccgccaataa atacccacgt ttgcgccatt tcatgagcat ctccagttac
    56821 tgatattcaa gagtgaaggt agccgtggca ttgatatgcc ccgcagtgac aggcacctgt
    56881 gtcgccatta gccgggcgta aaaattcagc gtatttggtt tacccggcgt cagggtcgtc
    56941 cacgaaagcg cggacgatgg agcattaagg ggtatttgat tttgctgctc attcagaagc
    57001 tgtattccca gtcccgaagc cgctgacacc gtattttcaa gtgcaagcag gttggcattg
    57061 tggctatctg caacaccggt aaacccaact tttacggcag aaacggcatt accacagggt
    57121 gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc caatgtcgtt aaattgcttc
    57181 gccgcgtttt ccatcaaatc aacagtaaaa ttggttgatt cagcggccac actacagccg
    57241 ttatctctga catagccgcg gatagtaatc gtgctatccg cagccaaaac gcgacttacc
    57301 gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa
    57361 cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa
    57421 ttggcgatac agtgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaaa
    57481 ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc
    57541 tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggttag cgtcatgagc
    57601 agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg agtgggaaca
    57661 acgttagcga ccgcgttatc taaatcgacg ttatcagcca gggtattggt atccagcgcc
    57721 actctgtttt cccgatattc ggtggcataa ggcagcacgg cataaccgcg ccagtcggta
    57781 cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt aacaagtacc
    57841 accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag taccccaccg
    57901 ctgactccgt aatagagctg cttaatatca tcgctatggc tgtaaccgat attggcattg
    57961 ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac ttccgctatt tccatcgcct
    58021 cccccggcat agccggtttg cacgctatag ctgaggttgt tgtcttccag caacgtaccg
    58081 tatacaccag ccagattggt catccgaccg ttgagatcgt gtgacatgct gtagctggca
    58141 ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg
    58201 acgttaagcg ctaacatctg atcccgtcct ttttgccagg cgtttttcgt caggctatag
    58261 ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg
    58321 acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgagcgc
    58381 ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt tgttataagc gaggttgtaa
    58441 tagtcggtga atttcggctt aacctgaata acaccatcct gtgtttcgat gttgtagcca
    58501 ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa
    58561 cccactaact gaatattcgt gcctgactca ttgagcgatt tgttatagag aaaacgcacc
    58621 gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgcgt catatccaca
    58681 gacagagcac ccagcgcccc catgtttttc ccgataccga aattaaaagc acgataacga
    58741 tccgccagtt gcgttccacc atatattgtc cagccagccg gaaggccgtg gagtaatgta
    58801 ctctggaaaa agcggggttt ttcctgttgc gcatttccac tacggtattc tcctgccgta
    58861 atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata gggtacggta
    58921 aaaatctgcg tgctgccgtc agcctctttg atcgttacct gcaagtcacc actattacct
    58981 gcggcataga tatcgttgat ggtaaaaggc cccggtggca ccgtactatt ataaatgtca
    59041 tacccatttt gtttaatagt gacctgtgca gtaccacgag caataccgtg gatcaccggg
    59101 gcaaatcctc tttgactatc gggtaacata ttgtcatctg aggccaattg tgcgccgcga
    59161 aagttaatac catcgaaaat atcgccctga gtataaccat cacccagcgt cagccgggaa
    59221 cgtaacggta ttatgtctcg ctcaagccag gtattgatat gctgccattt atttttgcta
    59281 cctgatgatc tgtcgctact gttataactc caggtggtat tgtcgcgtaa acgccacgca
    59341 ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc cccaatccga
    59401 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
    59461 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
    59521 aggttgagtc gctgctgacc aacatctaaa tgcgcagtag cgtcctggac cattgtggtt
    59581 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc catattcagc
    59641 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
    59701 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
    59761 tacgtccctg gcggtaattc ttgtccattt tcaaaacgcg ataaatcagc cacagcctgg
    59821 ggatcatccg ctaaaaagcg cgggttaaaa tagagttcgg cagatgacaa aggtgcctgt
    59881 gcggcaaaag cacaggcaac aaagagccgg acaaaaaaac cagccaaacg atgcttacga
    59941 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
    60001 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
    60061 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
    60121 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
    60181 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
    60241 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
    60301 tgatcgggtg gcaacgctaa tttagccgga cgatagtaca gtttaatgcg gctgataatt
    60361 gcgagctgta gcgtattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
    60421 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
    60481 cgcaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
    60541 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt gctattttca
    60601 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
    60661 gcacctaagg ccactcccgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
    60721 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
    60781 ctcactgtcc tgttcctgtt atcacattat ctgctgaaca attactgata ggttaaagag
    60841 aaccaggcct gtgcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
    60901 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
    60961 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta cgccaatatt ggtggctatc
    61021 cctggcccct ctcccacgga aagcacatcc ggatttttac catccgcgac accgtgaaac
    61081 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
    61141 accggtgcgc tatcttcccc taccgcgtga aaccggttac tgctgatttg ccccatattg
    61201 accgtcattt gtttatcacc agcttcaatc cggcaagttt ccgcaataat gacgccctga
    61261 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
    61321 ggcagcaacg acgctaatag aaataatctc ttccttttca tcgttatttt tatcacacaa
    61381 gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt gatactgaac
    61441 cttgaaggtc gcatccgcat tagcagcacc cggggttgcg gcaccggttg caaaataacg
    61501 cgcctggaac ggaatggtgt tggttccgtt attcagggtt gtttctgaac taaatgtcgc
    61561 accgtccagc gccagcgcag cacccgttct gtccaggatc tgcacaccaa cgtttgttgc
    61621 gctacccgca gctgaactct gcagagccag tacatcagta tgcgtagcat caattgccgt
    61681 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
    61741 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
    61801 gcctaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
    61861 ttccccttta aagtgaacgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
    61921 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
    61981 ttcctttaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac tttgttttgt
    62041 caacgagttt gactgccaac actgcacagt tttcccccaa a
//
LOCUS       DAOFSV010000010        59827 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.010, whole genome shotgun sequence.
ACCESSION   DAOFSV010000010 DAOFSV010000000
VERSION     DAOFSV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.010"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            23..1312
                     /gene="uraA"
                     /locus_tag="QBH27_000719"
     CDS             23..1312
                     /gene="uraA"
                     /locus_tag="QBH27_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDQ7013076.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            1362..2108
                     /gene="hda"
                     /locus_tag="QBH27_000720"
     CDS             1362..2108
                     /gene="hda"
                     /locus_tag="QBH27_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDQ7013077.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2246..2605)
                     /gene="arsC"
                     /locus_tag="QBH27_000721"
     CDS             complement(2246..2605)
                     /gene="arsC"
                     /locus_tag="QBH27_000721"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDQ7013078.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2626..4089)
                     /gene="bepA"
                     /locus_tag="QBH27_000722"
     CDS             complement(2626..4089)
                     /gene="bepA"
                     /locus_tag="QBH27_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDQ7013079.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4302..5363
                     /locus_tag="QBH27_000723"
     CDS             4302..5363
                     /locus_tag="QBH27_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDQ7013080.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(5461..5931)
                     /gene="bcp"
                     /locus_tag="QBH27_000724"
     CDS             complement(5461..5931)
                     /gene="bcp"
                     /locus_tag="QBH27_000724"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDQ7013081.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(5931..6503)
                     /locus_tag="QBH27_000725"
     CDS             complement(5931..6503)
                     /locus_tag="QBH27_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDQ7013082.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            6649..7527
                     /gene="dapA"
                     /locus_tag="QBH27_000726"
     CDS             6649..7527
                     /gene="dapA"
                     /locus_tag="QBH27_000726"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDQ7013083.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            7544..8578
                     /gene="bamC"
                     /locus_tag="QBH27_000727"
     CDS             7544..8578
                     /gene="bamC"
                     /locus_tag="QBH27_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDQ7013084.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            8791..9504
                     /gene="purC"
                     /locus_tag="QBH27_000728"
     CDS             8791..9504
                     /gene="purC"
                     /locus_tag="QBH27_000728"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDQ7013085.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            9672..10535
                     /locus_tag="QBH27_000729"
     CDS             9672..10535
                     /locus_tag="QBH27_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDQ7013086.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            10550..12565
                     /gene="tmcA"
                     /locus_tag="QBH27_000730"
     CDS             10550..12565
                     /gene="tmcA"
                     /locus_tag="QBH27_000730"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDQ7013087.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGGNEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPTRQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            12639..13337
                     /gene="ypfH"
                     /locus_tag="QBH27_000731"
     CDS             12639..13337
                     /gene="ypfH"
                     /locus_tag="QBH27_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ7013088.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(13418..13618)
                     /locus_tag="QBH27_000732"
     CDS             complement(13418..13618)
                     /locus_tag="QBH27_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDQ7013089.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(13646..14773)
                     /gene="dapE"
                     /locus_tag="QBH27_000733"
     CDS             complement(13646..14773)
                     /gene="dapE"
                     /locus_tag="QBH27_000733"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDQ7013090.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(14777..15133)
                     /locus_tag="QBH27_000734"
     CDS             complement(14777..15133)
                     /locus_tag="QBH27_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDQ7013091.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
     gene            15514..15573
                     /gene="ypfM"
                     /locus_tag="QBH27_000735"
     CDS             15514..15573
                     /gene="ypfM"
                     /locus_tag="QBH27_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDQ7013092.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(15672..18785)
                     /gene="acrD"
                     /locus_tag="QBH27_000736"
     CDS             complement(15672..18785)
                     /gene="acrD"
                     /locus_tag="QBH27_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDQ7013093.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(18949..20649)
                     /gene="narQ"
                     /locus_tag="QBH27_000737"
     CDS             complement(18949..20649)
                     /gene="narQ"
                     /locus_tag="QBH27_000737"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDQ7013094.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            20855..22834
                     /gene="aegA"
                     /locus_tag="QBH27_000738"
     CDS             20855..22834
                     /gene="aegA"
                     /locus_tag="QBH27_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDQ7013095.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            22902..23477
                     /gene="nudK"
                     /locus_tag="QBH27_000739"
     CDS             22902..23477
                     /gene="nudK"
                     /locus_tag="QBH27_000739"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDQ7013096.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            23603..24646
                     /locus_tag="QBH27_000740"
     CDS             23603..24646
                     /locus_tag="QBH27_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDQ7013097.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(24723..26726)
                     /gene="tkt"
                     /locus_tag="QBH27_000741"
     CDS             complement(24723..26726)
                     /gene="tkt"
                     /locus_tag="QBH27_000741"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ7013098.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(26746..27696)
                     /gene="tal"
                     /locus_tag="QBH27_000742"
     CDS             complement(26746..27696)
                     /gene="tal"
                     /locus_tag="QBH27_000742"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDQ7013099.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            27985..30264
                     /gene="maeB"
                     /locus_tag="QBH27_000743"
     CDS             27985..30264
                     /gene="maeB"
                     /locus_tag="QBH27_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDQ7013100.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            30557..30892
                     /gene="eutS"
                     /locus_tag="QBH27_000744"
     CDS             30557..30892
                     /gene="eutS"
                     /locus_tag="QBH27_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDQ7013101.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            30905..31384
                     /gene="eutP"
                     /locus_tag="QBH27_000745"
     CDS             30905..31384
                     /gene="eutP"
                     /locus_tag="QBH27_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDQ7013102.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            31359..32060
                     /gene="eutQ"
                     /locus_tag="QBH27_000746"
     CDS             31359..32060
                     /gene="eutQ"
                     /locus_tag="QBH27_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDQ7013103.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            32057..32860
                     /gene="eutT"
                     /locus_tag="QBH27_000747"
     CDS             32057..32860
                     /gene="eutT"
                     /locus_tag="QBH27_000747"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDQ7013104.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            32857..33873
                     /gene="pta"
                     /locus_tag="QBH27_000748"
     CDS             32857..33873
                     /gene="pta"
                     /locus_tag="QBH27_000748"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDQ7013105.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            33912..34205
                     /gene="eutM"
                     /locus_tag="QBH27_000749"
     CDS             33912..34205
                     /gene="eutM"
                     /locus_tag="QBH27_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDQ7013106.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            34312..34599
                     /gene="eutN"
                     /locus_tag="QBH27_000750"
     CDS             34312..34599
                     /gene="eutN"
                     /locus_tag="QBH27_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDQ7013107.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            34611..36014
                     /locus_tag="QBH27_000751"
     CDS             34611..36014
                     /locus_tag="QBH27_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDQ7013108.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGPTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNLAIVAAGGPENLLVTVTNPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            36025..36861
                     /gene="eutJ"
                     /locus_tag="QBH27_000752"
     CDS             36025..36861
                     /gene="eutJ"
                     /locus_tag="QBH27_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDQ7013109.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            36851..38038
                     /gene="eutG"
                     /locus_tag="QBH27_000753"
     CDS             36851..38038
                     /gene="eutG"
                     /locus_tag="QBH27_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDQ7013110.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            38255..39481
                     /gene="eutH"
                     /locus_tag="QBH27_000754"
     CDS             38255..39481
                     /gene="eutH"
                     /locus_tag="QBH27_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDQ7013111.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            39478..40881
                     /gene="eutA"
                     /locus_tag="QBH27_000755"
     CDS             39478..40881
                     /gene="eutA"
                     /locus_tag="QBH27_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDQ7013112.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGAQL
                     AQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            40893..42254
                     /gene="eutB"
                     /locus_tag="QBH27_000756"
     CDS             40893..42254
                     /gene="eutB"
                     /locus_tag="QBH27_000756"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDQ7013113.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCILAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            42275..43162
                     /gene="eutC"
                     /locus_tag="QBH27_000757"
     CDS             42275..43162
                     /gene="eutC"
                     /locus_tag="QBH27_000757"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDQ7013114.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHHADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            43172..43831
                     /gene="eutL"
                     /locus_tag="QBH27_000758"
     CDS             43172..43831
                     /gene="eutL"
                     /locus_tag="QBH27_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDQ7013115.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            43844..44344
                     /gene="eutK"
                     /locus_tag="QBH27_000759"
     CDS             43844..44344
                     /gene="eutK"
                     /locus_tag="QBH27_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDQ7013116.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            44390..45442
                     /gene="eutR"
                     /locus_tag="QBH27_000760"
     CDS             44390..45442
                     /gene="eutR"
                     /locus_tag="QBH27_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDQ7013117.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGELGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(45448..46347)
                     /gene="hemF"
                     /locus_tag="QBH27_000761"
     CDS             complement(45448..46347)
                     /gene="hemF"
                     /locus_tag="QBH27_000761"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDQ7013118.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(46351..47220)
                     /gene="amiA"
                     /locus_tag="QBH27_000762"
     CDS             complement(46351..47220)
                     /gene="amiA"
                     /locus_tag="QBH27_000762"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDQ7013119.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            47434..47859
                     /locus_tag="QBH27_000763"
     CDS             47434..47859
                     /locus_tag="QBH27_000763"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDQ7013120.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            47846..48295
                     /locus_tag="QBH27_000764"
     CDS             47846..48295
                     /locus_tag="QBH27_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDQ7013121.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            48356..48931
                     /locus_tag="QBH27_000765"
     CDS             48356..48931
                     /locus_tag="QBH27_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDQ7013122.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGNNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            49027..49926
                     /gene="yfeX"
                     /locus_tag="QBH27_000766"
     CDS             49027..49926
                     /gene="yfeX"
                     /locus_tag="QBH27_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDQ7013123.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(50104..51528)
                     /gene="murP"
                     /locus_tag="QBH27_000767"
     CDS             complement(50104..51528)
                     /gene="murP"
                     /locus_tag="QBH27_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDQ7013124.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(51532..52428)
                     /gene="murQ"
                     /locus_tag="QBH27_000768"
     CDS             complement(51532..52428)
                     /gene="murQ"
                     /locus_tag="QBH27_000768"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDQ7013125.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            52708..53499
                     /gene="ucpA"
                     /locus_tag="QBH27_000769"
     CDS             52708..53499
                     /gene="ucpA"
                     /locus_tag="QBH27_000769"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDQ7013126.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            53657..54673
                     /gene="cysP"
                     /locus_tag="QBH27_000770"
     CDS             53657..54673
                     /gene="cysP"
                     /locus_tag="QBH27_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDQ7013127.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            54673..55506
                     /gene="cysT"
                     /locus_tag="QBH27_000771"
     CDS             54673..55506
                     /gene="cysT"
                     /locus_tag="QBH27_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDQ7013128.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            55506..56381
                     /gene="cysW"
                     /locus_tag="QBH27_000772"
     CDS             55506..56381
                     /gene="cysW"
                     /locus_tag="QBH27_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDQ7013129.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            56371..57468
                     /gene="cysA"
                     /locus_tag="QBH27_000773"
     CDS             56371..57468
                     /gene="cysA"
                     /locus_tag="QBH27_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDQ7013130.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            57603..58514
                     /gene="cysM"
                     /locus_tag="QBH27_000774"
     CDS             57603..58514
                     /gene="cysM"
                     /locus_tag="QBH27_000774"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDQ7013131.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58517..58885)
                     /locus_tag="QBH27_000775"
     CDS             complement(58517..58885)
                     /locus_tag="QBH27_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDQ7013132.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            58990..>59827
                     /gene="pdxK"
                     /locus_tag="QBH27_000776"
                     /gene_synonym="thiJ"
     CDS             58990..>59827
                     /gene="pdxK"
                     /locus_tag="QBH27_000776"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDQ7013133.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPP"
BASE COUNT        13693 a        15376 c        16692 g        14066 t
ORIGIN      
        1 acataataca gaggataata ctatgacgcg ccgtgctatc ggggtgagtg aaagaccgcc
       61 acttttacag acaatcccgc ttagtttgca acatttgttc gccatgtttg gtgcaaccgt
      121 cctggtgccc gtcttatttc atattaaccc ggcgactgta ctgttattta acggtatagg
      181 aacactgctg tatctcttca tctgtaaagg gaaaattccg gcttatcttg gttccagctt
      241 tgcctttatt tcaccggtat tgttactgtt gccgttgggg tatgaagtcg cgctgggcgg
      301 ctttattatg tgcggcgtgc tgttctgcct ggtttctttt atcgtgaaga aagcggggac
      361 cggctggctg gacgtgctgt ttccacctgc ggcaatgggc gcaatcgttg ccgtcatcgg
      421 tctggagctg gcgggcgtag ctgccggtat ggcgggttta ctcccggctg aagggcaaac
      481 gccagactcc aaaaccatca tcatctctat taccaccctg gcggtcacgg ttttaggttc
      541 cgtgctgttt cgtggtttcc tggcaattat cccgatttta attggcgtgc tggtggggta
      601 cgcgctctct ttcgcaatgg gaattgtcga taccacgccg attattaatg ctcactggtt
      661 tgcgctgccg accctctata cgccgcgctt cgagtggttt gccattctga ctattctgcc
      721 agcggcgtta gtggttattg ccgaacacgt agggcacctg gtagtaacgg ctaatatcgt
      781 caaaaaagat ctgctgcgcg atccaggtct gcaccgttcg atgtttgcta acggcttgtc
      841 gaccgtgatc tccggcttct ttggctccac gccaaacacc acttacggag aaaacattgg
      901 cgtgatggcg atcacccgtg tttacagtac ctgggttatc ggcggtgcgg cgattttcgc
      961 tatcctgctt tcctgcgtcg gtaaactggc tgctgctatc cagatgattc cattgccggt
     1021 gatgggcggc gtttcgctgc tgctttacgg tgtcatcggt gcttccggta ttcgtgtgtt
     1081 gatcgaatcg aaagtggatt acaacaaagc acagaacctg atcctgactt ccgtgatttt
     1141 gatcatcggc gtcagtggcg cgaaggtaaa cattggggcg gcggagctga aaggtatggc
     1201 gctggcgacc atcgtcggta tcggcttaag tttgatcttc aaactgatta gtatgctgcg
     1261 ccctgaagaa gtggtgctgg acgcagaaga cgcggatata acagacaaat aatgtaccga
     1321 ccgggcagtg ttgctgcccg gttcaaacat catgggattc tgtggtaaac ttctcgcgat
     1381 tttgtgaaat cctggttgag gtatctctga acacaccggc acagctctct ttgccacttt
     1441 atcttcctga cgacgaaacc tttgcaagtt tctggccggg ggataactcc tctttactgg
     1501 ccgcgctgca aaacgtgctg cgtcaggaac atagcggtta catctatctc tgggcacgcg
     1561 aaggcgcggg gcgcagccat ctgctgcacg cggcttgcgc ggaattgtcg cagcgtggcg
     1621 atgcggtggg ctatgtcccg ctggataaac gcacctggtt tgttccggaa gtgctcgacg
     1681 gtatggagca tttgtcgctg gtctgtattg acaacattga gtgtattgcc ggcgatgagt
     1741 tgtgggagat ggcgattttc gatctctaca atcgaattct ggaatcgggc aaaacacggt
     1801 tgttgatcac cggcgatcgt ccaccgcggc agttgaatct gggattaccg gatctcgcgt
     1861 cgcgtctcga ctgggggcag atctacaaat tgcagccact ttctgatgaa gataagttgc
     1921 aggcgctaca gttacgcgcg cgtttgcgtg gttttgaact gccggaagat gtggggcgtt
     1981 tcttgctgaa gcggctcgac agagaaatgc gcacgttgtt tatgacgctg gatcagctgg
     2041 atcgtgcgtc gattaccgcg caacgcaagc tgaccattcc ttttgtgaaa gaaattctga
     2101 agttgtagat aaggtgttta ttgtcggatg cggcgcgaac gccttatccg acctacgcga
     2161 agttaatcca tagtattcgt aggccggata agacgcggca agcgtcgcat ccggcaatgt
     2221 gcttcaacta gcgatggagc acatcttaac ccacgatttc cagtacctgc tccggtggac
     2281 gaccaatccg tgctttgcca ttcgcgacca caatcgggcg ttccattagt ttcggattat
     2341 ccaccatcgc ctgaatcagc gcctcttcgc taagtgaact gtcagccagg ttcagttctt
     2401 tataaagatc ctctttttgg cgcatcaatt cacgagcgct attcatcccc agcattttca
     2461 gcaaatcacg cagcgttgcc gcatcggcgg gtgtctcaag gtagagcacc acttctggct
     2521 ccacgccgtt ttctttcagc aggttcagcg tttcccggct ctttgaacag cgtgggttat
     2581 ggtagatttt tacctgtttg gtcattgttc ttcctttaat gcgaattaca tcttggtata
     2641 aggcttaaag cgttcctgca gctggcgcaa ctggtcgatg cgcgcatcgt aacgcgcttg
     2701 ttgcaggctg cctaatttca cctgcgaact ggcgctactc aacaaggaaa tagcctgatc
     2761 gagtcgcccg gcgagcgcat aaccttctgc tcgcgcagcc agttcctgat cgcggttatt
     2821 tagtgcggct tccgcctgtg ccagtaaatc ccagccgttg ctgtcatctt tattgttaaa
     2881 agtgtagcga ttcagaatat tggccgcttc ctgtggttgc cccccttgca gataagcgtt
     2941 cgccaggttg agctgcaaca ccggattggt gcgcaaatcg cgggcatttt tcaggcgatt
     3001 gatagcgtca ttggctttgt tttgcccaag atcgatatcg gtagccagat cgagatacca
     3061 tgcgttgcca ggttctgctg ccagtaacgg ttgcagcgtt ttacgcgctt cgtcgtattt
     3121 attggcttcc atcgcctgta gagcacgacc atattgcgcc gctcgttgct gacgaacgtt
     3181 tcctttcgcc cattcatcca gcaaatcact ggtgagctgg ttacgtccgg aattatacat
     3241 ccccagtgtg cgcgctttcg ccagatagaa atcttccgac gactgcacca ccatcgggcg
     3301 catctgatta gcacggttgc gggcatctgc cagacgactt tccggcaacg ggtgagtcaa
     3361 taaaatttcc ggcggacgcg aggagtaacg cgcctgatcg agtaattttt cgaggaaggt
     3421 cggcatcgcc tgcggatcga atcccgagcg ttgcagcacc tgaataccaa tgcggtccgc
     3481 ttcctgttca ttttgctggg tgaaactgat catcccctgg cgcgttcccg ccagtgtacc
     3541 ggtcagcgcc gccatccccg cctgcggact ggccatcgcc agtaaaatag aacctaacgc
     3601 gccgacccag gtcagcggcg cgctgcgctg ctgatcttcc atcgctcgcg ccaggtgacg
     3661 ttgggtgacg tgggagattt cgtgcgccat aactgaagcc agttgacttt cgttatcgga
     3721 ataacggaac agggcagagt gcagcaccac gttgccgcca aagaaagcaa aggcgttaat
     3781 ttcgtcgttg ttgatcagaa agaaatgaaa cggtgtctta accgaattgg catgcgaaac
     3841 cagacgcatt cctagcgaat taatatattg cgttaatagc gggtcattaa ttaacggcgc
     3901 gctgccgcgt aactggcgga cataatagtc gcccatctgc atttcctgac caatggaaag
     3961 tgtgcttcct gcggaggttc ccatatccgg caaggtgtct gcgctgtcgg caaacgccgg
     4021 ggctacctga ccaatggtca tagcagcaat gagggttgca accaggtttt ttttcaactg
     4081 cctgaacata acctctatcc tgtatttctg gagatagtca tttgaccgat actgtgaaat
     4141 atcgttccca ccgcgccgcc tttgagtgta acgacgaaga ttagcccact caaataactt
     4201 ttcagtttcc tgaatccgtc ataaaaagcg aagtcttttt tgtgacattt cgatacaatt
     4261 gcggatcgca atcccgctct tgaggttcag gtaaggtctg tatgctcgaa atgttgatgc
     4321 aatggtatcg ccgccgtttt agcgacccgg aagcgattgc cttgctggtt attttagtcg
     4381 ccggatttgg cattatcttt ttctttagtg gcctgcttgc tccgttgctg gtggctattg
     4441 tgctggccta tttgctggag tggccaaccg tgcgcctgca atctattggc tgttcccgcc
     4501 gctgggcgac gtcgattgta ttggtggttt tcgtcggtat attgctactg atggcgttcg
     4561 tggttctgcc tattgcctgg cagcagggca tctacttaat ccgcgatatg ccggggatgc
     4621 tcaataagct ttctgacttt gccgccacgt tgccgcgccg ctatccggcg ttaatggatg
     4681 cgggcattat tgatgcaatg gccgaaaata tgcgcagtcg gatgctgacc atgggcgatt
     4741 cggtggtgaa aatttccctc gcctcgttgg tcggtttgct gaccattgcc gtctatctgg
     4801 tgctggtgcc attgatggtc ttcttcctgc tgaaagacaa agagcagatg ctgaacgccg
     4861 ttcgccgggt gctgccgcgc aaccgtggac tggcaggaca ggtgtggaag gagatgaatc
     4921 aacaaatcac caactatatc cgcggcaaag tgctggagat gatcgtggta gggatcgcca
     4981 cctggctggg gttcttgctc tttgggctga actattcgct gctgctggcg gtgctggtcg
     5041 gcttctcggt acttattccg tacattggcg catttgtggt gaccattccg gtggttggcg
     5101 tggcgctatt ccagtttggt gctggcacgg aattctggag ctgcttcgcg gtgtatctga
     5161 ttattcaggc gctggacggc aacctgttag taccggtgtt gttctccgaa gcggttaacc
     5221 tgcatcctct ggtgattatt ttatcggtgg tgatcttcgg tggtttgtgg ggattctggg
     5281 gcgtattctt cgctattcca ttggcgacgc tgatcaaagc cgtgattcac gcctggcctg
     5341 atgggcaaat cgcgcaagac tgaccttcag cgaaaaattt gaaacgagtg ttgcagggca
     5401 gtgagaagat ttgagatgtg ctggccgcaa gcgcagccag cacggaatgg agcaaagtaa
     5461 tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg ttttgaaatc
     5521 gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc gatgaatacc
     5581 atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt cgcacacctg
     5641 gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa aacgggagag
     5701 tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt tcaactcatc
     5761 catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg ctttcgggta
     5821 gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt ctccgtcttg
     5881 atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca ttacttaact
     5941 ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg tgcattgagt
     6001 tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga tgcggggctg
     6061 tgggcagtta tctgaatatg taactgcgca gcccgttcat tttcagcagg ttgcgtgcgc
     6121 gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa gcgttcaatt
     6181 aaatgcgggg agtctgccac atcgacctga acccagacag atgctggcat tggtggacgc
     6241 ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gttcggcacc tttcaacggt
     6301 aacgttgatt caatcagggt gattgcattc catgaaccgg aaagcagcat gataaacgtg
     6361 aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca actactgaca
     6421 tgacgggtga tggtgttcac tattccaggg cgatcggcac ccaacgcagt gatcaccaga
     6481 taatgttgcg atgacagtgt caaactggtt attccttcaa ggggtgagtt gttcttaagg
     6541 aaagcataaa aaaaacatgc atacaacaat cagaacggtt ctgtctgctt gcttttaatg
     6601 ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat gttcacggga
     6661 agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg ggctagcttg
     6721 aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc tgttggcacc
     6781 actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat gatgacgctg
     6841 gagctggctg acgggcgcat tccggtgatt gccgggaccg gtgctaacgc tactgcggaa
     6901 gccattagcc tgacgcagcg cttcaatgac agcggtatcg tcggctgcct gacggtaacc
     6961 ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc catcgctgaa
     7021 catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg cgatctgctc
     7081 ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa agaggcaaca
     7141 gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt tgttctgctg
     7201 agcggcgatg atgcgagcgc gctggacttc atgcaattag gcggtcatgg ggttatttcc
     7261 gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc agcagaaggg
     7321 cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa caaactattt
     7381 gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct tgtggcgacc
     7441 gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac ggtcagagcg
     7501 gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt actctgttca
     7561 aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg ctgcctgtag
     7621 ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg aagcggcacc
     7681 gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg gcgattatgc
     7741 aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc gtccaccagc
     7801 ccagccgttg gcactggttt ctggcgcgcg tacccagttc acgggcgata ccgcttcatt
     7861 gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg tgctgcaggc
     7921 gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca ccgattgggt
     7981 acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa tctctgttaa
     8041 gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac aggcgggcaa
     8101 accggttgca gacgcggcgt ccatgcagcg ttacagcacg gagatgatga acgttatttc
     8161 cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc gtgcctccac
     8221 cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg tcgtacgcgg
     8281 gccgttcaat gtggtttggc aacgtctgcc agctgcgctg gaaaaagtgg gcatgaaagt
     8341 gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc tgtctgacag
     8401 cgaatggcac gaactgggcg cgagcgatcc aggtctggca tccggtgact ataaactgca
     8461 ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag gtcatactct
     8521 gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta gcaagtaaaa
     8581 atacagggct ggagtcatcc ggcccttttt tctgatatga tacgcaaacg tgtgcgtcgg
     8641 cagaaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac gtaggtcgga
     8701 taaggcgttc acgccgcatc cgacaaaaca tccggcacac cagacagcaa aagattttaa
     8761 aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt gtatcgtggt
     8821 aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga attccgcaat
     8881 gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg tatggtgaac
     8941 aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc gactcaaatg
     9001 gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt gccggttgag
     9061 tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat cgaagaaggt
     9121 attgagctga acccgccgct gttcgatctg ttcctgaaaa acgacgccat gcacgatccg
     9181 atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa cctggcgcgt
     9241 atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga tgatgctggt
     9301 ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggcgaagt ggttctgggc
     9361 gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacgctgga gaaaatggac
     9421 aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc cgtcgcccgc
     9481 cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc tgctggcacg
     9541 gcaagacaac cgctctcata agatgagtat tgaacgctca ttcctccgcc atttcatccc
     9601 gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat gaatataatg
     9661 ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa gacaggcgca
     9721 atagctccgg cggtccgtca atgggcggtc ccggttttcg cctgccaagc ggtaaaggcg
     9781 ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat ttaaccgggt
     9841 tgatgaccgg acagccggtt tcccaacagc aatcaacgcg gtcaattagc cctaatgaag
     9901 acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc tggggacagc
     9961 aattcgagaa gatgggtaaa acctatcagc aaccgaagct ggtcatgtac cgtggaatga
    10021 cgcgtaccgg ctgcggggcg ggccagtcca taatggggcc attctattgc ccggcggatg
    10081 gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt ggcgcggatg
    10141 gcgattttgc ccaggggtac gttatcgctc atgaagtcgg tcatcatgtg cagaaactgt
    10201 taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg gaagtaaacc
    10261 gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg gggcatagta
    10321 tgcagcagca aggtgttctg gaaaccggcg atctggaaga ggcgctgaac gcggcgcagg
    10381 ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca gacagtttca
    10441 ctcatggcac ttctcagcaa cgctacagct ggtttaaacg tggtttcgac agcggcgatc
    10501 cggcacaatg caatactttt ggtaaaagca tttaacttcc ggggcaggga tggctgaact
    10561 gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa gggatccgcc gcttgctggt
    10621 gttgagcggg gaagagcgtt ggtgttttga tcatgcgctt aagttgcgtg atgccttacc
    10681 tggcgactgg ctgtggattt cgccgcagcc agatgctgaa aaccactgtt ctccctcggc
    10741 actacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc gccacggctt
    10801 tgatgccgct gcctttgcgg cacttagcgg aacgttgaaa gcgggaagct ggctggtgtt
    10861 gttactccct gtatgggatg agtgggaaaa ccaacctgat gccgactcgc tgcgctggag
    10921 tgattgccct gaccctattg cgacgccgca ttttgtccag catttcaaac gcgtacttac
    10981 ggcgaataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg cgcattttac
    11041 tccccgtact gactggcacc ccgctaccgg cgcaccacaa ccagaacaac agcaactctt
    11101 acagcagcta ctgaccatgc cgccgggtgt ggcagcggta actgctgcgc gtgggcgcgg
    11161 taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga ttgtcaccgc
    11221 tcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt ttcgctttat
    11281 tgcgccggat gccttgttag ccagcgatga gcaagccgac tggctggtgg tcgatgaagc
    11341 cgcagctata cctgcgccgt tgttgtatca actggtatcg cgttttcctc gaacgttgtt
    11401 aactactacg gtgcagggct acgaaggcac cggacgtggt tttttgctga aattttgcgc
    11461 tcgctttccg catttacacc gttttgaact acaacagccg atccgttggg cgcagggatg
    11521 cccgctggaa aaaatggtta gtgaggcact ggtgtttgac gatgaaaact tcacccatac
    11581 accacaaggc aatatcgtta tttccgcatt tgaacagacg ttatggcgaa gcgagccaga
    11641 aacgccgtta aaggtttatc agttattgtc tggtgcgcac taccggacct cgccgctgga
    11701 tttacgccgc atgatggatg caccaggtca acatttttta caggcggctg gcggaaacga
    11761 gattgccgga gcgctgtggc tggtggatga gggcggatta tctcaagaac tcagtcaggc
    11821 ggtatgggcg ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc tggcggcgca
    11881 cggcagcaat ccactggcgg cgacattgcg cggacggcgg gttagccgga tagcagttca
    11941 tccgactcgt cagcgggaag gcacagggcg acagcttatt gctggtgctt tgcaatatat
    12001 tcatgacctc gactatcttt cggtgagttt tggttacacc gaggagttat ggcgtttctg
    12061 gcaacgctgc ggttttgtgc tggtgcgaat gggtaatcat cgtgaagcca gcagcggttg
    12121 ctatacggcg atggcgctgt taccgatgag tgatgcgggt aaacagctgg ctgaacgtga
    12181 gcattaccgt ttacgtcgcg atgcgcaagc tctcgcgcag tggaatggcg aaatgcttcc
    12241 tgttgatcca ctaaacgatg ccgtcctttc tgacgacgac tggcttgaac tggccggttt
    12301 tgctttcact catcgtccgc tattaacgtc gttaggttgc ttattgcgtc tgctacaaac
    12361 cagcgaactg gcattaccgg cgctgcgtgg gcgtttacag aaaaacgcca gtgacgcgca
    12421 gttatgtacc acacttaaac tttcaggccg taagctgtta ctggttcgcc agcgggaaga
    12481 ggccgcgcag gcgctattcg cacttgatga tgttcgcact gagcgtctgc gcgatcgcat
    12541 aacgcaatgg caattttttc actgactcct tcaattttct cccatggtca ttgtgagtca
    12601 gcggcgtaaa attaatgaca tcagttaagg agttcgccat gaaacatgac cattttgttg
    12661 ttcaaagccc ggataaacca gcacaacagt tgttactgct tttccacggt gtcggggata
    12721 acccggtggc aatgggggaa ataggtagct ggtttgcgcc gctgtttcct gatgcgctgg
    12781 tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc ggggcgtcag tggttttcgg
    12841 tgcagggtat tacggaagat aatcgccagg cgcgtgtgaa tgccatcatg ccgacgttta
    12901 ttgagacggt gcgctactgg cagaaacaaa gcggagtagg ggctaacgcc acggcgctaa
    12961 tcggtttctc acaaggcgcg attatggcgc tggagagcat taaagccgaa ccgggccttg
    13021 cttcgcgcgt cattgccttc aacggacgct acgcgagcct gccggaaacc gcgtctacag
    13081 ccacaacaat tcacttgatt catggcggtg aagatccggt tatcgatctg gcgcatgcgg
    13141 ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt gacgctggat atcgtggagg
    13201 atttaggcca tgcgatcgac aatcgcagta tgcaatttgc cctggatcac ttgcgttata
    13261 ccattccgaa acattatttc gacgaagcgc taagcggcgg taagcctggc gatgatgatg
    13321 tgattgaaat gatgtaataa tagccgggta ggtctgataa gactgtatca ggcatcatgg
    13381 ccggatgcgg cgttaacgcc ttatccggcc acttagctta cttcttcggt tgatcctttt
    13441 tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc acgatgcggg ggaagctccg
    13501 gcttgttggc gagaaatttc ttatggtcga cgcgcttgag atctttaatc acgttcagca
    13561 ggacgcctac caaaaagaca atcaccagaa tccaccaata tttagccagc cagtccatgc
    13621 ttatttcctc ttgcagaacc actcatcagg cgacgagctg ttccatgata cgttgataca
    13681 tacgggcaag tagctgcagg tcggcagcgt tcacacattc attgatttta tgaatcgtgg
    13741 cattgaccgg cccgagttcc accacctgcg cccccatgcg ggcaataaag cgcccgtcgg
    13801 acgttccgcc tgtggtcagt agctgcggtt taatttcatt atagtgctca accgcgttaa
    13861 cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg ctgcccggaa agccaccaat
    13921 ccaccgtata gcgcagttga tgtttttcaa gcagggcaag cacctgcgct ttgatcatct
    13981 catccgtcag ttcggtgcta aagcggaagt taaactgcac aaacagttca cctggaataa
    14041 cgttgttact gcccgttccc gcctgaatat tggcaatctg catactggtc gccgggaaga
    14101 agtcattacc ctgatcccac tcaatagcca ctaattcatt aatgaaaggt gctgcgcgat
    14161 gtaccggatt gtcagccaga tgtgggtagg caacatgccc ctgaacgcca tgaatggtaa
    14221 ggttgcaggt taacgatccg cgacgaccat ttttcaccac atcacctacc acttcgatac
    14281 tcgacggttc gccaaccaga cagtaatcga gacgctcatt acgcgccatt aacgcttcga
    14341 cgacttttac cgtaccgttg tgggcactag cttcttcatc agaggtgatc agaaatgcca
    14401 gtcgccctgt atggttggga tgttgtgcga caaagcgttc tgccgctacc accatcgccg
    14461 ccagcgagcc tttcatatct gccgcacctc gcccaaataa catgccgtca cgaatggttg
    14521 gttcaaatgg tggattgatc caacggtcgg cgtcgccagg cggcaccacg tcggtatgcc
    14581 cggcaaaggc taacgtttca ccctgcccgc gccatgccca aaaattctgc gtatcggcaa
    14641 agtccatgcg ttcaacggta aaaccgatcg cctgcaaacg ttcaatcaac aaagcctggc
    14701 atcctgcgtc atcaggactc agggaagggc ggcgaataag ctgttgtgtc agctcaataa
    14761 ccgggcacga catagactac acctcatgga aaaattgctg ataactggat tcactgaaac
    14821 ccagcagcat aggcttacca ggcgcgcaga gcaatggacg tttgataatt gcaggcattt
    14881 cagtcattaa tgtcgccgca gaggccgcat cggtgatttt attgcgggtg gtttcgtcca
    14941 gtttacgcca ggttgtacca cgggtgttga gtaacgcttc ccagcctaat tcgttgataa
    15001 aaccgttcaa taattcgctg tccaatccat cgacgcggta atcatgaaaa cgatagtcga
    15061 tgttattggc ttccagccaa cggcgagcct ttttaatggt gtcacaattt ttgatgccgt
    15121 aaagtgtaac cataatgaat cctttttcga tttatgagaa taaatactca tttaagggtg
    15181 aaaacatttt gcctgcaaat gattatcagg aaatataaca ttatcgaagt attcaaatta
    15241 acaactatta aataacatta aacattttta acgtaaacat taagaatatt catcttgtta
    15301 tctgtctgta ttttaaccag aattaaatca gtattagatg ccgtatgaat atctcgaaat
    15361 gttgcggtgt atcacataca aatcatcacc gcaggaccat agtatttaca taaggtttgt
    15421 cccccggagg tagtcatcgc agcaatcgag taaattagct tcaccacggt atattgtttc
    15481 cgtagaatac ccataattat aagagaggtt gttatgattg aacgtgaact ggggaactgg
    15541 aaagacttta tcgaagttat gcttcgtaag taattcctga gacaaagtac aaacagcaag
    15601 aacccgctga gcaggttctt aatcggaaag ttgaaaaggg cgacacattg gcatgtcgcc
    15661 ttttttattg cttattccgg gcgcggcttc agcgggaagc ggcggcgcac cagcacaaag
    15721 aacagcggca cgaagtaaat agccagaata gtggccgaaa tcatcccgcc cattacgcca
    15781 gtacccaccg catgctgacc accggaaccg gcacctgtgc tggttgccat tggcaatacg
    15841 ccgaagataa atgccagcga ggtcatcaga atcgggcgta aacgctgacg gcaggcgtgg
    15901 agcgtcgctt caaacaggtc gtggcctttt tggttcatct cgttagcaaa ctcgacgatc
    15961 aggatggcgt ttttcgccga taaaccaatg accgttaaca ggcccacctg gaagtaaaca
    16021 tcgttttcca gcccgcgcat ccaggttgcc agcagcgcgc cgataacccc cagcggcacg
    16081 accagcatta ccgagaacgg caccgaccag ctttcataca gcgcagccag acacaggaat
    16141 accaccagca aggaaatggc gtacagcgcc ggagcctgcg cgccggaaag ccgctcctga
    16201 tacgacatcg ccgtccactc cagaccaaag ccgtttggca actgcttcac taacgattcc
    16261 ataatatcca tcgccgtacc ggtactgacc cccggtgcgg cttccccaac aatctcaacc
    16321 gcagaataac cgttatagcg ttccagacgc ggcgagcctg tctcccagcg tgaggtcgcg
    16381 aaagcagaga agggcaccat gccgccatct ttatttcgga cataccagag attgatgtca
    16441 tctggcagca tgcgatacgg cgcagctgcc tgcacataga ctttcttcac gcgaccgcga
    16501 tccataaagt cattcacata gctcgaaccc caggcggttt gcagtgtgtc gttaatatcg
    16561 tcgatagcaa cgcccagcgc ctgagcttta cgctggtcga tatcaatctg caactgcgga
    16621 ctgtcgtcga ggccgttatg gcgcacacgg gttagctccg ggttttccgc tgccagcgcc
    16681 agcaactggt ttcgtgctgc catcagcgca tcgtgacccg ctccagcgtg gtcctgcaac
    16741 tccatatcaa aacccgcaga actaccaaga ccgctaattg ctggcgggct gctggcgata
    16801 acgcgagctt ctttaatctt gttaaacgcc ttcgttgcac gctcgataat ggcaaacgag
    16861 gtgccggtct tactgtcgcg ttcgctccag tctttcaggc ggataaacat tcgcgccacg
    16921 ttttgcccgt tccccccagg gccagaaccc acggtggcaa acaccgacat gatgttgtct
    16981 ttttcatggg tgaagtaata tttctcgatt tgctcaacga ctttcagggt ctgttgctgg
    17041 gttgaaccgc tgggcaactg taccgaggta gtaaacatgc cacggtcttc cagcggtaag
    17101 aacgacgtcg gcaaacgcag gaacaggaac accatgccgc caagcagcag gacataaatc
    17161 acaatccagc gcaggctacg gtggagaatt ttcgccaccc ctttttcgta gcgttcggcg
    17221 ttgcggttaa acatctggtt aaaccaggca aaaaagcctt tttgcccgtg atgctcacct
    17281 tttttcagtg gcttaagtag tgtggcacac agagccggag tgaggatcat cgctaccagt
    17341 actgacagca ccatcgccgc aacaatggta atagagaact ggcgatagat ggcaccggtg
    17401 gtgccgccga agaaggccat tggcacaaat accgccgaaa gcaccatcgc aatcccgacc
    17461 agtgccccct ggatctgccc catcgattta cgtgtggctt cgcgaggagt gagtccttcc
    17521 tcactcataa tacgttcgac gttttccacc accacgatgg catcatccac cagcagaccg
    17581 atcgccagca ccatcgcgaa catggttaag gtgttgacgc tatagccgaa ggcataaagc
    17641 accgagaagg ttcccatcaa caccaccggc acggcgatag tgggtatcag cgtggcgcgg
    17701 aagttttgca ggaacagata cataacgagg aaaaccaggg cgatagcttc cagaagcgtt
    17761 ttcaccacgt cttcaatgga ggcttttaca aaagaggtgg tttcatacgc caccttgtat
    17821 tccagtccgt gtggaaaata ctgcgccagc tcgtcgagac gattgagcac cagctccgct
    17881 gtcgccattt cgttcgcgcc ggaggccagt tttaccccca gcccggaggc tggcttacca
    17941 ttgaagcggc taagataatc gtatttctcc gcccccattt cgacggtggc gacatcgccc
    18001 agccttacct ctgagccgtc ctgattcacc cgcaaggtga tatcgcggaa ctgttctggt
    18061 gtttgcagca gtgactgggc gttaatggtg gcgttgagcg cctgcttatc gacggaaggc
    18121 gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg attcaatggc atcggtgaca
    18181 tctttagccg tcatctggaa actgttgagt ttcgccgggt ccagccagat acgcatggaa
    18241 tattgcgaac cataggcatc gatatccccg acgccattca cgcggcttaa cgggtcctga
    18301 atattactgg caacataatc ggcaatatcc tgtttatcca tcgaaccatc ggtagagacg
    18361 aaggcaatgg tcagaatgtt ggtatcgccg gttttacgca ccgtcacgcc ctgattttgc
    18421 accgcctgcg gtaactttcg catggctgat tgcagctggt tttgtacttg ctgcacggct
    18481 tcgtccggat cggtgcctgc tttaaaactt aacgtgacag atgcctgacc ggtgccgctg
    18541 ctctgagatg acatatacat cagattatcg aggccggtca tattttgctc gataacctgg
    18601 gtcacggtgt tttccagcgt ctgggccgat gcgccgggat agttagcggt cactcgcaca
    18661 ttcggtggcg cgagatcggg gtattgttca acgggcaatg aaaaaatcgc cagggtacct
    18721 gtcagacaca acaggattgc cagcacccag gcaaaaatgg ggcgatcaat aaagaaattc
    18781 gccattaaaa gaggacctcg tgtttctgcg tatcgttgta ggcttggctt gcttcactgt
    18841 agcggcaagg tacgagccaa tcgtggagaa aaaaaggagt taatgtaaat tatcatgccc
    18901 ttttatactg aaccttaagt gccactattc tttggtcaat aagaggcatt acatcagctg
    18961 actttcctca ccctccgcag agcgaaagct aatactcact aacgtgccgc cgccggaagg
    19021 ttgtgaaaaa gtcagcgtcc cacccaatct ttccgcgcgt tcgcgcatga tattcagacc
    19081 ataatggcct tcgggttctt tcggttcacc gatgccaatc ccgttgtcgc gaatatagac
    19141 tgtgtgattg ccatccggcg cggtgacgca gctaacggcg atttcgctgg cgttggcgtg
    19201 cttcatcgca ttcagcaccg cttcgcgaat aatttgcaac aaatgcacct gcatttgcgc
    19261 atccagcgcc agggttggca gacggcagtc gagggtcagt ttggcgctgg tttgattttg
    19321 taacgtatcc agcatttccc tcaacgcgga ggggagatcc gcctgctgca gcgtcaggcg
    19381 gaaagtagtc aacagctcgc gtaactgccg ataagcatca ttcaacgcct gggaaaaatc
    19441 ggccatgata ctttgtgcgg tggcgttatc ttccggtatc gaacgcttca gtaacgtcaa
    19501 ctggatacgt aagtaagaaa gtacctgagc cagcgagtcg tgcaattcgc gggcgatggt
    19561 cgcacgttct tccatcaaca ataactgctg aaaatgcttc tgcgcctgat taaagtacaa
    19621 accgcgtccc agcatcgacg aaacgctgtt aagcagcggt tctgatgatg aaacgtgact
    19681 attttgccag tgcagttctc cgtaaaccgt ctcttgcatt gtcaccggta aaatctgcat
    19741 cggcaattcc gggtttggct gcccttcgct aatccgccag ttgtcaccga catttaactc
    19801 cagatattca gccgcttcat tgtcgcgaac aatctgcaaa atatggcgga aacaatgcac
    19861 atcaatctgg ctggtgttta gcgcctgcga acactgatac aacacctcca gacgacgctt
    19921 ggcctcgtgg agatcgcggg tcttttcttc tactgacgcc tccagcgaac ggtacaattt
    19981 atgcagctcg ctcgacatct ggttaaaggt ttttgcaagc aggccaagct cattcggcag
    20041 gctggtatcc agcggcggcg agtcgaactg cccgtgttca atacgctgac tggcggtaac
    20101 cagctgattc agcggggcaa ccacctgatg gcgtatgcgg cgcaaagtaa aaaagaccag
    20161 cgtgaaaata ccgatgccgc cagccaggga aattgccacc accagcagca ttttgcgttc
    20221 agcgtagtgc tgtaaagcca gcacgaacag gtctatctga ttaacataat tattaatatt
    20281 ggcctgatac cacggcaagt cgcccttgct gagccgatta ttcatctcca gccagttggc
    20341 attcagatgc gcatagcggg tttttactgc ctctggcaca taccagacgt tgagattggt
    20401 taataccggt gaatgcagtg cctgctgaaa taactggcga tgtgcattga gttgtggact
    20461 gccactttgc aagtcgtagc ccaggcggta actctgcata cgcagcgatc cggcaatatt
    20521 gatagcctca gcgtcgcgta aactgctcgc cagcgttagc agagcgatac ccgtggaaag
    20581 aatcgacagc agcacaatgt aaaaaaaggc ccgggccaga ctggccgaga cgggtcgttt
    20641 aacaatcaca cgcgtcttct ccacaaaaaa tcattaatct tccagttcgc tgaaacgcaa
    20701 tttagtcagt gatgggatta tttgatctgc cacagtttat agcgaaatag ttacttattc
    20761 tgggcacatg aacattgccg attcccctgt aagtcgggta ataacaacac tcatataaag
    20821 aataaggttt ttacaaccaa aaaagaaggt cgttatgaat cgttttatta tggccaacag
    20881 tcagcaatgt ctgggttgtc atgcttgtga aatcgcctgt gtcatggctc acaatgacga
    20941 gcaacatgtc ctgagccaac accattttca tccccgaatt acggttatca aacatcagca
    21001 gcaacgtagt gccgtgacct gtcaccattg tgaagatgcg ccctgcgccc gtagctgccc
    21061 taatggcgca atcagccacg ttgatgacag cattcaggtc aatcagcaaa agtgtattgg
    21121 ctgtaaatcc tgcgtggtgg cctgtccatt tggtacgatg caaatcgttc tgacacccgt
    21181 tgcggcagga aaagtaaaag ccacggcgca taaatgcgac ctttgtgcag ggcgcgaaaa
    21241 cggtcctgcc tgtgtcgaaa attgcccggc ggacgcgctg caactggtca ctgacgccgc
    21301 actctccggc atggcgaaat cccgccgctt gcgcaccgca cgtcaggaac atcaaccgtg
    21361 gcatgccagt accgcggcgc aagaaatgcc ggtaatgagt aaagtcgagc aaatgcaggc
    21421 aacgcccgcg cgtggcgagc cagataaact ggcgattgaa gcgcgcaaaa ccggttttga
    21481 tgaaatttat ctgccattcc gtgccgatca ggcacaacgg gaagcctcgc gctgccttaa
    21541 gtgcggcgag cacagcgttt gtgaatggac ctgcccgctg cataaccata taccgcagtg
    21601 gattgaactg gtgaaagccg gaaacatcga cgccgccgtc gagctttctc accagaccaa
    21661 caccctgccg gaaattaccg gacgcgtttg cccgcaagac cgtttgtgtg aaggtgcctg
    21721 tactattcgc gatgagcacg gcgcagtaac tatcggtaac attgaacgct acatttcaga
    21781 tcaggcgttg gcgaaaggtt ggcgtcctga cttaagccat gtcaccaaag tggacaagcg
    21841 ggtggcgatt atcggtgcgg gtccggcagg gctggcctgt gcagatgttc tgacccgcaa
    21901 tggcgtggcg gtgacggtgt acgatcgcca tccagaaatc ggtggcttgc tcactttcgg
    21961 cattccttct ttcaaactgg ataaatccct gctggcacgc cgtcgggaaa tcttcagcgc
    22021 gatggggatt cacttcgaac tcaattgtga agtgggtaaa gatgtctctt tgaattcgct
    22081 tttggaacaa tacgacgcgg tcttcgttgg cgtaggcact taccgttcca tgaaagcggg
    22141 tttacccaat gaagatgcgc cgggcgttta tgacgcgctg ccgttcctca tcgccaacac
    22201 taaacaggtg atgggactcg aagagctacc ggaagagccg tttatcaata ccgccggact
    22261 taacgtcgtg gtactgggcg gcggcgacac cgcgatggac tgtgtgcgta ccgcgctgcg
    22321 ccatggcgcg agtaacgtca cctgcgctta tcgtcgtgat gaagctaaca tgccaggctc
    22381 gaagaaagag gtgaagaacg cccgtgaaga gggggccaac ttcgaattta acgtccagcc
    22441 ggtggcgctt gagctgaatg aacaaggtca cgtctgcggg attcgtttcc tgcgcacgcg
    22501 tcttggcgag ccggatgccc agggacgtcg gcgtccagtg ccggtggaag gcagtgaatt
    22561 tgtcatgcca gccgatgcgg tgattatggc gtttggcttc aatccgcacg ggatgccgtg
    22621 gctggagtcg cacggtgtaa cggtagacaa atggggccgc atcatcgcgg atgtggaaag
    22681 ccagtaccgt taccagacca cgaatccgaa aatcttcgcc ggtggtgacg ccgtgcgtgg
    22741 tgcggatctg gtggttaccg caatggcaga aggacgtcat gcggcacagg ggatcattga
    22801 ctggctgggc gtaaaatcag tcaaatctca ctgatagcct gcgcagacaa acccgacttc
    22861 acagcgtaag ataattgttc atttcgcgct gtggagtcgg tatgacgcaa caaatcaccc
    22921 tcgttaaaga caaaattctc tccgataact atttcaccct gcacaacatt acttacgatc
    22981 tcacgcgcaa agatggcgaa gttatccgcc ataaacgtga agtttacgat cgcggtaatg
    23041 gtgcgacgat cctcctgtac aacgcgaaga aaaagaccgt ggttctgatt cgccagttcc
    23101 gcgtcgctac ctgggttaat ggcaatgaaa gcgggcaact gattgaaacc tgcgccgggc
    23161 tgctggataa cgacgaaccg gaagtgtgca ttcgcaaaga agcgattgaa gagacgggct
    23221 atgaagttgg cgaagtccgc aaattatttg aactgtatat gtcgccaggc ggtgtgactg
    23281 agctaatcca cttttttatc gccgaataca gtgacagcca gcgcgctaac gccggtggtg
    23341 gtgtcgaaga tgaagatatt gaagtgcttg agctgccgtt cagccaggcg ctggagatga
    23401 ttaaaaccgg cgagatacgt gacggtaaga cggtgttatt gcttaactat ttgcaaatgt
    23461 cacatttaat ggactgaaaa ataacaataa tatttcgttg tttattattg gctcaatccg
    23521 acaaatccga ttgagccgcg ctacctgcgc aacgaagata cgacttgtgc tgtttgtttg
    23581 aacttctggg gtcgtaccgt ccatgcgata tcgcattttc cttctctttt ttttcgcttt
    23641 gttgccgatg tctttggtgt gggcggcacc agcgcaacgg gcgttttccg actggcaggt
    23701 cacctgtaat aatcaaaatt tctgtgtagc gcgcaatacg ggcgatcata atggactggt
    23761 gatgaccctg agccgcagcg ccggggcgca taccgatgcc gttttacgta ttgagcgcgg
    23821 cgggttgaag tcgccggatg cgtcagaagg ggagatagcg ccacggctgc tgttagatgg
    23881 tgagccgttg gcgttaagtg gtgacaagtg gcggatttca ccatggttat tagtaaccga
    23941 tgatacggca accatcaccg cgtttttgca gatgattcag gaagggaggg cgatcacctt
    24001 acgcgacggc aatcagacta tttctctgag tggcttaaaa gcggcgttgt tgtttattga
    24061 tgcccagcaa aagcgcgttg gcagtgagac cgcgtggata aagaaagggg acgaaccgcc
    24121 gctcagcgta ccgccagcgc ctgcgctgaa agaggtcgcg gtggttaacc caacgccgac
    24181 gccactctca ctcgaagaac gtaacgattt gctggattat ggaaactggc ggatgaatgg
    24241 tctgcgctgt tcgcttgatc cgttacgtcg tgaggtgaat gtcactgcgc tgaccgatga
    24301 taaagcgctg atgatgatta gctgtgaggc aggggcctat aacaccattg atttggcatg
    24361 gattgtgtcg cgtaaaaagc cgcaggcctc gcgcccggta cggttgcgtt tgccgttcaa
    24421 caacggtcag gagaccaatg aactggaact gatgaacgca acattcgatg aaaaatcgcg
    24481 tgaactggtg accttagcaa aagggcgcgg attaagcgat tgtggcattc aggcgcgctg
    24541 gcgctttgac ggtcagcgtt ttcgcctggt gcgttacgcc gcagaaccca cctgcgataa
    24601 ctggcatggg ccagacgcct ggcccacgtt gtggatcacc cgttaaacaa aatggcgggg
    24661 acatgtagac cggataagac gcaccagcgt cacatccgac aatcagcatc cggcgatcac
    24721 catcaggcac ctttcactcc cagtacctta tgcgcttttg ccacaatatt ctcggcggta
    24781 aagccaaaga acgggaacag cttatccgcc ggagctgatt ccccataacc tgtcatcccg
    24841 acaattgccc ctttcaggcc aacatatttg taccagtaat cggcaatacc cgcttctact
    24901 gccacgcggg cagaaacatt tgaaggcagt accgactccc gatatttctc atcctgggca
    24961 tcgaaaatat cggtcgaggg cagggaaact acgcgaacat tgcgaccttc tcccgctaat
    25021 ttctctgccg cttgcagggt gatttccatc tctgaaccgg tggcaatcag aataatatct
    25081 ggcttaccgc cgctgtcttt cagcacataa ccgccacgag caatctcttt aacctgatcc
    25141 ggtgttcgtt ctacctgtgc cagattctgc ctggagagga tcagtgctgt cggtccgttg
    25201 tggcgctcaa ccgccagctt ccagccaacc gccgcttcca cctgatcgca cggtcgccag
    25261 gtgctgaaat ttggcgttaa gcgcagacta gccagttgct caacagcctg gtgcgtcgga
    25321 ccatcttcgc ccagaccaat tgagtcgtgg gtataaacca taatctgccg cgctttcatc
    25381 agtgccgcca tccgcgcggc gttacgggcg tattcaacaa acatcaggaa cgtcgcggta
    25441 tacggcacaa agccgccgtg gtgcgcgatg ccgttggcga tagcggtcat accaaattca
    25501 cgcaccccgt agtgaatgta gttgcccgcc ggatcttcct tcagcgaaac agaacctttc
    25561 cagatggtca ggttgctggg agccagatcc gccgaaccgc cgagcaactc aggcagcatc
    25621 ggcccgtaag cgttaagcgt attttgcgaa gccttacggg tagcgatttt cgccggattc
    25681 gcctgcaact cattgatata tttctgagtc gttttctccc agtccttcgg caaaccgccg
    25741 ctcatccgac gggtaaactc ttctgccagt tgcggatgag cctttttata ggcggcaaac
    25801 ttctcattcc agctctgctg cgctttttcg cctttttcgc gggcatccca ggcgtgataa
    25861 atctctttag ggatctcaaa tggcggatgg tgccagccca gtttttgccg tgccagagct
    25921 acttcttctt cccccagcgg tgcgccgtgc gcctcttcct tacctgcttt attcggcgaa
    25981 ccaaagccaa tcaccgtacg gcagataatc agcgacggct tatctttcac gctttgcgct
    26041 tcaaggatcg cttccttcac cgcctgcgga tcgtggccgt cgatctcgtg gaccacatgc
    26101 cagtgatagg cttcaaaacg ttttgccgta tcgtcggtaa accagccttc ggtttcaccg
    26161 tcgatggaaa taccgttgtg atcgtaaaaa ccaatcagct tgcccagccc cagcgtgccc
    26221 gccagcgaac agacttcgtg agagatacct tccatcaggc agccgtcgcc cataaacaca
    26281 taggtgaagt gatcgacaat ttcatgatcc ggctgattaa attgcgccgc cagcgtacgc
    26341 tctgctatcg ccagcccgac ggcgttcgcc aaaccttgtc caagaggacc ggtggtggtt
    26401 tcaacacctg gcgtataacc gatttccggg tgaccagggg ttttcgaatg caactgacgg
    26461 aagttcttca gttcttccag tggcaggtcg taaccggtca gatgcagcaa actgtagagc
    26521 agcatcgacg cgtgaccgtt ggaaagaata aagcggtcgc gatcatacca ggttgggtcg
    26581 gtagggttat gtttaagaaa atcgttccac agcacttcgg caatatcagc catgcccatc
    26641 ggcgcgccgg gatgaccaga attggctttt tgtaccgcat ccatactgag tgcgcgaatc
    26701 gcattggcaa ggtcttttcg ggacatataa cactccgtgg ctggtttata gtttggcggc
    26761 aagaagatct tccagtttgc gttgatcaac ggcgaacaga cgaatgcctt cagacagttt
    26821 ttctaccgcc atcgcatcct gattgtgctc ccaacggaac tctgcttcgc tcatgggagc
    26881 tgggcgtggg aacgtctgag aaggtgggat taatttacgt accactggcg aaactttttc
    26941 ctgtagctcc ttcagcaaat tcggtgcgat agtcagtcgg tcgcagccgg ttaaggcgag
    27001 gatttgttcg gtgcgacgga agctcgcgcc catcacaatg gtttcatagt ggtgttgctt
    27061 atagtagtcg tagatattgc gtactgattt aacgcctgga tcttcttcca ccacatacgg
    27121 gtccatcggc ttgcgtgcct gataccagtc ataaatacgc ccgacaaatg gcgaaatcag
    27181 aaaaacgcct gcttctgcac aggcccgcgc ctgtgcaaaa gaaaacagca gcgtcaggtt
    27241 gcagttaata ccttcttttt ccagctcttc tgccgcgcga attccttccc aggtcgaagc
    27301 cagcttgatc agaatgcgcg atttctcaac gccttgttgc tgatacaaat ccaccagatg
    27361 gcgcgccttc tcaatactct tttctttatc aaaagagagg cgtgcatcaa cttctgttga
    27421 cacgcgaccg ggtacgattt tgagaatttc agcaccgaaa ttgaccgcca gtttgtcaca
    27481 cgctgcgacc acctgttgtt cctgggtctt gccatttttt ttaccccagg cgatagcatc
    27541 gtctattaaa tgctcatatt gtgataatcc ggcagccttg agtaacagcg aaggattggt
    27601 ggtggcatcc tggggatgat aatggcgaat ggactcaata tcgccgctgt ctgccacgac
    27661 agtggtgaac tgtttgatgc cgtctaactc gttcatagga aatactcctt gaaaagtaaa
    27721 gtgttagatg agtgcgttaa ttcacacttc tgagaaattt cgctaaacgc atcaaaaaag
    27781 catagcagac aggcatggta ttgctggatt aagcaggtaa catcagtgtt atatgattat
    27841 taccaaaaca ttatattaat ggtgagagtt tggacttgct caaagtctgt agactccggc
    27901 agggtaataa tgtgcgccac gttgtgggca ggggctgttg cccacacaat ttatttgtga
    27961 acgttacgtg aaaggaacaa ccaaatggat gaccagttaa aacaaagtgc acttgatttc
    28021 catgaatttc cagttccagg gaaaatccag gtttctccaa ccaagcctct ggcaacgcag
    28081 cgcgatctgg cgctggccta ctcaccaggc gttgccgcac cttgtcttga aatcgaaaaa
    28141 gacccgttaa aagcctacaa atataccgcg cgcggtaacc tggtagcggt gatctctaac
    28201 ggtacggcgg tgctggggtt aggcaacatt ggcgcgctgg caggcaaacc ggtgatggaa
    28261 ggcaagggcg ttctgtttaa gaaattcgcc gggattgatg tatttgacat tgaagttgat
    28321 gaactcgacc cggacaaatt tattgaagtc gtcgctgcgc tcgaaccgac cttcggcggc
    28381 atcaacctcg aagacattaa agcgccagaa tgtttctata ttgaacagaa actgcgcgag
    28441 cggatgaata ttccggtatt ccacgacgat cagcacggca cggcaattat cagcactgcc
    28501 gccatcctca acggcttgcg cgtggtggag aaaaacatct ccgacgtgcg gatggtggtt
    28561 tctggcgcgg gtgccgcagc aatcgcctgt atgaacctgc tggtagcgct gggtctgcaa
    28621 aaacataaca tcgtggtttg cgattcaaaa ggcgttatct atcagggccg tgagccaaac
    28681 atggcggaaa ccaaagccgc atatgcggtg gtggatgacg gcaaacgaac cctcgatgat
    28741 gtgattgaag gcgcggatat tttcctcggc tgttccggcc cgaaagtgct gacccaggaa
    28801 atggtgaaga aaatggcccg tgcgccaatg atcctggcgc tggcgaaccc ggaaccggaa
    28861 attctgccgc cgctggcgaa agaagtgcgt ccggatgcca tcatctgtac cggtcgttcc
    28921 gactacccga accaggtgaa caacgtcctt tgcttcccgt ttatcttccg tggcgcgctg
    28981 gacgttggcg caaccgccat caacgaagag atgaaactgg cggcggtacg cgcgattgca
    29041 gaactggccc atgcggaaca gagtgaagtg gtggcttcag cgtatggcga tcaggatctg
    29101 agctttggtc cggaatacat cattccaaaa ccgtttgatc cgcgcttgat cgttaagatc
    29161 gctcctgcgg tggcgaaagc ggcgatggag tcgggcgtgg cgacgcgtcc gattgctgat
    29221 ttcgacgtct acatcgacaa gctgacggag ttcgtttaca aaaccaacct gtttatgaag
    29281 ccgattttct cccaggctcg caaagcgccg aagcgcgtag ttctgccaga aggggaagag
    29341 gcgcgcgttc tacatgccac tcaggaactg gtaacgctgg gactggcgaa accgatcctt
    29401 attggtcgtc cgaacgtgat cgaaatgcgc attcagaaac tgggcttgca gatcaaggcg
    29461 ggcgttgatt ttgagatcgt caataacgaa tccgatccgc gctttaagga gtactggacc
    29521 gaatacttcc agatcatgaa gcgtcgcggt gtcactcagg agcaggcgca gcgtgcgctg
    29581 atcagtaacc cgacagtgat cggcgcgatc atggttcagc gtggcgaagc cgatgcaatg
    29641 atttgcggta cggtgggcga ttatcatgaa cactttagcg tggtgaaaaa tgtctttggt
    29701 tatcgcgatg gcgttcacac cgcaggtgca atgaacgcgc tgctgttgcc gagtggtaac
    29761 acctttattg ccgataccta cgtcaatgat gagcctgatg cagaagagct ggcggagatc
    29821 accttgatgg cggcagaaac tgttcgtcgt tttggtattg agccgcgtgt ggctctgttg
    29881 tcgcactcca actttggttc ttctgactgc ccgtcgtcga gcaaaatgcg tcaggcgctg
    29941 gaactggtca gggaacgtgc gccagaactg atgattgatg gtgaaatgca cggcgatgca
    30001 gcgctggtgg aagcgattcg caacgaccgt atgccggaca gccctttgaa aggttccgct
    30061 aatattctgg tgatgccgaa catggaagct gcccgcatta gttacaactt actgcgtgtt
    30121 tccagctcgg aaggtgtgac tgtcggcccg gtgctgatgg gggtggcgaa gccggttcac
    30181 gtgttaacgc cgatcgcatc ggtacgtcgt atcgtcaaca tggtggcgct ggccgtggtt
    30241 gaagcgcaaa cccaaccgct gtaattcttt tttaactctc acgcttaccc tgaatattca
    30301 gggtaagcgg tttagctgca atatattagt aaagtttatt actgagtttg cgaataataa
    30361 aaaaatcagt atataaaata tctcgatatt attttattta tattcatgcg ttgtatatga
    30421 aaatttatgc accacagcaa atatctctcc attcttagtg atctacctca ccttttaaac
    30481 gcgcttgccg aattttgtta tttactctga cgaaaaattg tcacgataca cgaaagtttt
    30541 tcacaggcgg cgactcatgg ataaagaacg catcattcag gaatttgtgc ccggtaaaca
    30601 ggtcacactg gcgcatctca tcgcgcatcc cggcgaggaa ctggcgaaga aaatcggcgt
    30661 tccggatgcg ggcgcaatcg gcattatgac gctaactccc ggagaaacgg cgatgatcgc
    30721 cggtgattta gcgttgaaag ccgccgatgt gcatatcggt tttctcgatc gttttagtgg
    30781 cgcgctggtg atttatggct cggtgggcgc agtagaagaa gcgttatcac aaaccgtcag
    30841 tggtttagga cgcttattaa attatacgct ttgtgaaatg accaaaagtt aatacgaggc
    30901 attcatgaaa cgaattgcgt ttgtgggtac cgtcggggca ggaaaaacaa cgttatttaa
    30961 tgcgttgcag gggaattata ccctcgcaag aaaaacacag gccgtggaat ttaatgataa
    31021 tggcgatatt gatacgccgg gtgaatattt tagccatccc cgctggtatc acgccttaat
    31081 taccacgcta caggatgtgg atatgctgat ctatgtccac ggcgcgaatg acccggaaag
    31141 tcggttgcct gccgggttgc tggatattgg cgtcagcaaa cggcaaatcg ccgtcatcag
    31201 taaaacggac atgccagatg ctgacgtcgc cgcaacgcga aagttattgc ttgagacggg
    31261 cttcgaagag ccgattttcg agctaaacag ccacgacccg caaagcgtgc agcaactggt
    31321 ggattatctg gcatctctca ccaaacagga ggaagcaggt gaaaaaactc atcacagcga
    31381 atgatattcg tgaagcacac gcgcgcggcg aactggcaat gtccgtggtt ctgcgcgcca
    31441 gcattattac cccggaagct cgtgaagtgg ctgatctgtt gggttttacc atcaccgaat
    31501 gtgatgaatc gatcccggta acggcgtctg ttcccgccag cgcatcagcc gataaaaccg
    31561 aaagccagcg catccgtgaa accattatcg cccaactgcc ggaaggtcag tttaccgaaa
    31621 gcctggtcgc gcagctgatg gaaaaagtga tgaaggaaaa acagtcgctg gaacagggtg
    31681 cactgcagcc gagctttaaa tcggtgaccg gcaaaggcgg catcaaagtt attgatggca
    31741 gcagcgtcaa gtttggtcgt ttcgatggcg cggaaccgca ctgcgtaggc ttgactgatc
    31801 tcgtgaccgg agacgacggc agcagtatgg ccgctggttt catgcagtgg gagaacgcct
    31861 tctttccgtg gaccctgaac tacgacgaaa tcgacatggt gctggaaggg gagctgcatg
    31921 ttcgccacga aggcgaaact atgatcgcca aagcgggcga tgtgatgttt atcccgaaag
    31981 gctccagcat cgaatttggt acgacatcca gcgtgaaatt cctgtatgtc gcctggccgg
    32041 ctaactggca atccttatga aagatttcat caccgaagca tggctaagag cgaaccatac
    32101 gctcagcgaa ggcgcagaga tccatctccc tgcggacagt cgcctgacgc cctctgcccg
    32161 ggagttactg gaaagccgcc atctgcgcat caagtttatt gacgagcagg gccgcctgtt
    32221 tgttgacgat gaacagcagc agccgcagcc cgttcacggg ctgaccagta gcgatgaaca
    32281 tccgcaggcg tgctgcgaac tgtgccgcca gccggtggca aaaaagccgg atacgctgac
    32341 tcacctgtcg gcggaaaaaa tggttgccaa aagcgatccg cgtctgggtt ttcgcgctgt
    32401 tctcgacagc accattgcgc tggcagtgtg gctgcaaatt gaactggcag aaccgtggca
    32461 gccgtggctg gcggacatcc gctcgcgtct tggcaacatt atgcgtgccg atgcgctggg
    32521 ggaaccgctg ggcgagcaag cgattgtcgg gctttctgac gaagatctgc atcggctttc
    32581 tcaccagccg ctgcgttatc tcgaccacga tcatctggtg ccggaagcca gccacgggcg
    32641 cgatgcggcg ttgctcaatc tgctacgcac caaagtgcgg gaaacagaaa ccgtagcggc
    32701 gcaggtcttt atcacccgca gctttgaagt attacgcccg gacattttgc aggcgctgaa
    32761 ccgcctttcc agcacggtct acgtgatgat gatcctgagc gtaacgaagc agccgctaac
    32821 ggtgaagcag atccaacaac gactggggga aacgcaatga ttattaaacg ttgtcgtgaa
    32881 ctggcgttgc gagcgcccgc cagagtagtt tttccggatg cgttagatca acgtgtgctg
    32941 aaagctgcgc aatatttaca tcaacaaggt ctggcaacgc ccattctggt cgccaatccg
    33001 tttgaacttc gtcagtttgc gctcagtcac ggcgtggcga tggacgggct acaggtgata
    33061 gatccgcatg gcaacctcgc aatgcgggaa gaatttgctc atcgctggct ggcccgcgcg
    33121 ggcgaaaaaa cgccgccgga tgcgctggaa aaacttaccg atccgctgat gttcgccgct
    33181 gcaatggtca gcgccggtaa agcggatgtc tgtatcgcgg gcaacctctc ttccacggcg
    33241 aatgtgctgc gtgccggatt acgcattatc ggcttgcagc caggctgtaa aacgctctca
    33301 tccattttcc tgatgctgcc acagtacagc ggtccggcgt tgggttttgc cgattgcagc
    33361 gtggtgccac agccgacggc ggcacaactg gcggatatcg cgcttgccag cgccgaaacc
    33421 tggcgcgcca tcaccggaga agaaccgcgc gtggcgatgc tgtcgttttc cagcaacggt
    33481 agtgcccgtc acccctgtgt tgccaacgtc cagcaggcga cagaaatcgt ccgtgagcgc
    33541 gcaccaaagc tggttgtcga tggcgagttg cagtttgacg ccgccttcgt gccggaagtg
    33601 gcggcgcaaa aagcgcctgc cagcccgtta cagggcaaag ctaatgtgat ggtttttccg
    33661 tcgctggaag ccggaaatat tggctacaaa atcgcacaac gacttggcgg atatcgtgcc
    33721 gtcgggccat tgatacaagg acttgccgcg ccgatgcacg atctctctcg tggttgtagc
    33781 gtgcaggaaa ttatcgaact ggcgctggtg gcagctgtgc cgcgtcagac agaagtgaac
    33841 cgcgaaagca gtttacaaac actggttgaa tgaacggtcc cgttctggac cctgataaag
    33901 aggaaagcac aatggaagca ttaggaatga tcgaaacccg gggcctggtt gcactgattg
    33961 aggcttctga cgcgatggtt aaagctgcgc gcgtcaagct ggttggtgtt aagcagattg
    34021 gcggcggcct gtgtactgcc atggttcgtg gtgatgttgc agcctgtaaa gccgcgaccg
    34081 atgcaggtgc cgcagcggca cagcgtatcg gcgagctggt atctgtacac gttatcccgc
    34141 gtccgcacgg cgacctggaa gaagtcttcc cgatcggcct gaaaggcgat agcagcaacc
    34201 tgtaatcacc ctgtgtgacg tcgattcggg cgtcacgctc tctccttttt cattttacct
    34261 tctgcggagg gtagggggag cttttacctg aaatacgcca cggaggcggg tatgaaactg
    34321 gcagtcgtca ctggacaaat tgtttgtacc gtacgccatc acggactggc gcatgacaaa
    34381 ttgctgatgg tggaaatgat tgatccacaa ggtaatcctg acgggcaatg cgccgtcgcc
    34441 atcgacaata ttggcgcggg aaccggggag tgggtgttac tggtgagtgg cagttccgcc
    34501 cgccaggcgc ataaaagcga aacgtcaccg gtcgatctgt gcgtgattgg cattgtcgat
    34561 gaggtggtgt ctggcggtca ggtcattttc cacaaataag gcagaacatc atgaatcaac
    34621 aggatattga acaggtggtg aaagcggtac tgctgaaaat gcaaagcagt gacacgccgc
    34681 ccgccgccgt tcatgagatg ggcgttttcg cgtccctgga tgacgccgtt gcggcagcca
    34741 aagtcgccca gcaagggtta aaaagcgtgg caatgcgcca gttagccatt gctgccattc
    34801 gtgaagcagg cgaaaaacac gccagagatt tagcggaact tgccgtcagt gaaaccggca
    34861 tggggcgcgt tgaagataaa tttgccaaaa acgtcgctca ggcgcgcggc acaccaggcg
    34921 ttgagtgcct ctctccgcaa gtgctgactg gcgacaacgg cccgaccctg attgaaaacg
    34981 caccctgggg cgtggtggct tcagtgacgc cttccactaa cccggcggca accgtaatta
    35041 acaacgccat cagcctgatt gccgcgggca acagcgtcat ttttgccccg catccggcgg
    35101 cgaaaaaagt ctcccagcgg gcgattacgc tgctcaacct ggcgattgtt gccgcaggcg
    35161 ggccggaaaa cttactggtt accgtaacaa atccggatat cgaaaccgcg caacgcttgt
    35221 tcaagtttcc gggcatcggc cttttggtgg tgaccggcgg cgaagcggta gtagaagcgg
    35281 cgcgtaaaca caccaataaa cgtctgattg ccgcaggcgc tggcaacccg ccggtagtgg
    35341 tggatgaaac cgccgacctc gcccgtgctg ctcagtccat cgtcaaaggc gcttctttcg
    35401 ataacaacat catttgtgcc gacgaaaagg tactgattgt tgttgatagc gtagccgatg
    35461 aactgatgcg tctgatggaa ggccagcatg cggtgaaact gaccgcagag caggcgcagc
    35521 aactgcaatc ggtgttgctg aaaaatatcg acgagcgcgg aaaaggcacc gtcagccgtg
    35581 actgggttgg tcgcgatgcg gcgaaaatcg cggcggcaat cggccttaaa gttccggaac
    35641 aaacgcgact gctgtttgta gaaaccaccg cagaacatcc gtttgccgtg accgaactga
    35701 tgatgccggt gctgcccgtc gtgcgcgtcg ccaacgtggc ggatgccatt gcgctggcgg
    35761 tgaaactgga aggcggttgc caccacacgg cggcaatgca ctcgcgcaat atcgaaaaca
    35821 tgaaccagat ggcgaacgcc attgatacca gcattttcgt taagaacgga ccgtgtattg
    35881 ccgggctggg gctgggcggg gaaggctgga ccacgatgac catcaccacg ccaaccggtg
    35941 aaggggtaac cagcgcgcgg acgtttgtcc gtctgcgtcg ctgtgtatta gtcgatgcgt
    36001 ttcgcattgt ttaaggagta accgatggcg cacgatgaac aatggctcac cccacgtctg
    36061 caaacggcgg caacgctgtg taaccagacg cccgccgcga cggaatctcc gctgtggctg
    36121 ggggtggatc tgggcacctg cgatgtggtg tcgatggttg tcgaccgcga cggtcagccg
    36181 gtggcggtat gcctcgactg ggccgatgtg gtccgcgacg gcatcgtctg ggatttcttc
    36241 ggtgcggtca ccattgttcg tcgccatctc gacacccttg agcagcaatt cggtcgccgt
    36301 tttagccatg cggcgacctc atttcctcct ggtacggacc cgcgtatttc catcaacgtg
    36361 ctggagtctg ccggactgga agtgagccat gtgctggatg agccaaccgc agttgcggat
    36421 ctgctgcaac tggacaacgc cggtgtagtg gatatcggcg gcggcactac cggcattgcc
    36481 atcgtgaaaa agggcaaagt gacgtactcg gcggatgaag ccaccggcgg acatcacatc
    36541 tctctgactc tcgccggaaa tcgccgtatt tcgctggaag aggcagagca gtacaaacgc
    36601 ggtcacggtg acgagatttg gcccgcggtg aaaccggtgt acgaaaaaat ggcggacatt
    36661 gtggcccgtc atattgaagg gcagggcatt acggatttat ggctggcggg cggttcctgt
    36721 atgcaaccgg gcgtagcggc gctgttttgc aaacaatttc cggcgttaca ggtgcattta
    36781 ccgcagcaca gtttgtttat gaccccgctg gcaatcgcca gtagtgggcg agagaaagcg
    36841 gaggggctat atgcaaaatg aattgcagac cgcgctcttt caggcgttcg ataccctgaa
    36901 tctgcaacgg gtaaaaacat ttagcgttcc accggtgacg ctttgcggtc cgggcgcggt
    36961 gagcagttgt gggcagcaag cgcaaacgcg tgggttgaaa catctgttcg tgatggcaga
    37021 cagctttttg catcaggcag ggatgaccgc tgggctgacg cgtagcctgg ccgttaaagg
    37081 tatcgccatg acgctctggc catgtccggt gggcgaaccg tgcatcaccg acgtgtgtgc
    37141 agccgtggcg cagttgcgtg aatcaggttg tgacggggtg atcgcgtttg gcggcggctc
    37201 ggtgctggat gcggcgaaag ccgtggcgtt gctggtgacg aacccggata gcacgctggc
    37261 agaaatgtca gaaaccagcg ttctgcaacc gcgcctgccg ttgattgcca ttccaacgac
    37321 cgccggaacc ggctctgaaa ccaccaacgt gacggtgatt atcgatgcgg taagcgggcg
    37381 caaacaggtg ttagcccatg cctcgctgat gccggatgtc gcgattctcg acgctgtgct
    37441 aaccgaaggc gtgccgtcgc atgtcacggc gatgactggc attgatgcgt taacccatgc
    37501 cattgaagcg tacagcgcgc tgaacgctac accgtttacc gacagcctgg cgattggcgc
    37561 cattgcgatg attggtcaat cgctgccgaa agcggtgggc tacggtcacg accttgtcgc
    37621 ccgcgagagc atgttgctgg cttcatgtat ggcgggaatg gcgttttcca gcgccgggct
    37681 tgggttatgc cacgcgatgg cgcatcagcc aggggcggcg ctgcatattc cgcacggtct
    37741 ggcgaacgcc atgttgctgc caacggtgat ggaatttaac cggatggttt gtcgtgatcg
    37801 gtttagtcag attggtcggg cgctgcgaac taaaaaatcc gacgatcgtg acgctattaa
    37861 tgcggtaagt gagctgatag cggaagttgg gatcaacaag cggctggctg atgtgggtgc
    37921 aacaaccggg cattacggtg catgggcgca ggccgcgcag gaagatattt gtctgcgcag
    37981 taacccgcgt accgccagcc tcgagcagat tgtcggcctg tacgcagcgg cgcaataaat
    38041 gccggatgcg acgcttgccg cgtcttatcc ggcctacggg attgcacatg tagggcggat
    38101 aaggcgttca cgccgcatcc gccagtggcg cggtgcaatt gccggatgcg acgcttgccg
    38161 cgtcttatct ggccttcggg attgcacatg tagggcggat aaggcgttca cgccgcatcc
    38221 gccaataaat aacaactaac agggagtaaa ggcgatggga attaacgaaa tcatcatgta
    38281 catcatgatg ttctttatgc tgatagctgc cgtagacagg atcctgtcgc agttcggcgg
    38341 ttctgctcgt ttcctcggta agttcggtaa aagtatcgaa ggatccggcg gtcagttcga
    38401 agaaggcttt atggcaatgg gcgctctggg cctggctatg gtcggtatga ccgcactggc
    38461 accggtactg gcccacgttc tcggaccggt gattatcccg gtttacgaaa tgctcggcgc
    38521 aaacccgtca atgttcgccg gaacactgct ggcgtgcgat atgggcggct tcttcctcgc
    38581 caaagagctg gcgggcggtg acgtagcagc gtggctatac tctgggttaa ttctcgggtc
    38641 gatgatgggg ccaacgattg tgttttccat tccggtggcg ctcggcatta tcgaaccttc
    38701 tgaccgtcgt tatctggcgc tcggcgtgct ggcgggcata gtgaccattc cgattggctg
    38761 tatcgctggt ggtctggtag cgatgtactc cggtgtgcag atcaacggcc agcctgttga
    38821 attcaccttt gcgctgatcc tgatgaacat gatcccggta cttatcgttg cggtgctggt
    38881 ggcgctgggg ctgaaattca tcccggaaaa aatgatcaac ggcttccaga tcttcgccaa
    38941 attcctcgtt gcattgatca ccctcggtct tgccgctgcg gtagtgaaat tcctccttgg
    39001 ctgggaactg atcccgggcc ttgatcctat ctttatggcc cctggcgata aacccggtga
    39061 agtgatgcgc gccattgaag ttatcggctc gatctcctgc gttctgttag gggcgtatcc
    39121 aatggtactg ctgctgactc gctggtttga aaaaccgctg atgagcgtcg gtaaggtgct
    39181 gaatatgaac aatatcgcgg cagccggcat ggtggcaacg cttgccaaca acattccgat
    39241 gtttggcatg atgaagcaga tggatacccg cggcaaagtc atcaactgtg ccttcgccgt
    39301 ttccgctgct ttcgcgctgg gcgaccattt aggcttcgcc gctgccaaca tgaacgccat
    39361 gatcttcccg atgattgtcg gcaaattgat cggcggcgta acggcaattg gcgtggcgat
    39421 gatgctggtg ccaaaagaag acgcgagcgc ggctaaaacc gaagcggagg cgcaatcgtg
    39481 aacactcgcc agctactgag cgtcggtatc gatatcggca ccaccaccac ccaggtgatc
    39541 ttctcccacc tggagctggt taaccgtgcg gcggtgtcgc aggtgccgcg ctacgaattc
    39601 atcaaacgcg aaattagctg gcaaagcccg gtgttcttta cccctgtcga taaacagggt
    39661 ggtttaaaag aagcggaact gaaaacctta atcctcgagc aatatcaggc tgcgggcatt
    39721 gcgccggaaa gcgttgattc tggtgccatc atcatcaccg gtgaaagcgc gaaaacccgc
    39781 aacgctcgcc cggcggtgat ggcgctctct cggtcgctgg gcgattttgt cgtcgccagc
    39841 gccgggccgc atctcgaatc cgtgatcgcc ggtcacggtg ccggggcgca aaccctttct
    39901 gaacaacggc tgtgtcgggt actgaatatc gacatcggcg gtggcaccgc gaactacgcc
    39961 ctgttcgatg ccggaaaaat cagcggcact gcctgcctca acgtcggcgg tcgcctgctg
    40021 gaaaccgaca gccaggggcg cgtggtttac gctcataaac cggggcagat gattgtggat
    40081 gagtgcttcg gcccaggcac tgacgcccgt tcgctgaccg gcgcgcaatt ggcgcaggtt
    40141 acccgccgga tggcggaact gattgtcgaa gtgattgacg gaacgctctc gccgctcgcg
    40201 caggcattga tgcaaaccgg tttgctgccc gcaggtgtta cgcccgaaat cattacgctt
    40261 tctggcgggg taggcgaatg ttatcgccac cagcccgccg acccgttctg ttttgccgat
    40321 atcggcccgc ttctggcaac ggcgctgcat gaccatccgc gtctgcgtga gatgaacgtg
    40381 cagtttccgg cgcaaaccgt gcgcgccacg gtgattggcg cgggtgcaca taccctttcg
    40441 ctctctggca gcacaatatg gctggagggc gtacaactgc cgctgcgcaa tttgccggtg
    40501 gcgatcccga ttgatgaaac ggatctggtc agtgcctggc aacaggcgct gattcaactg
    40561 gatctttgcc ccaaaactga cgcgtacgtg ctggcgcttc ccgcctcgct gcctgtgcgt
    40621 tacgccgcgg tactgacggt catcaacgcg ctggtcgatt tcgtcgcgcg ttttccgaat
    40681 ccgcatcccc tgctggtggt ggccgggcag gactttggta aagctctggg catgttgttg
    40741 cgcccacagc tacaacaact cccgttggca gtcattgacg aagtgattgt ccgcgcgggg
    40801 gactatatcg acattggtac gcctcttttt ggcggatcgg ttgtgccggt gacggtgaaa
    40861 tcactcgcat ttccttcctg agggaacgac ttatgaaact aaagaccaca ttgttcggca
    40921 atgtatatca gtttaaggat gtaaaagagg tgctggctaa agccaacgaa ctgcgttcgg
    40981 gggatgtgct ggcgggcgtt gctgcggcaa gctcacagga gcgcgtggcg gcaaagcagg
    41041 tgttgtcgga aatgactgta gcggacatcc gcaataatcc ggtgattgcc tatgaagatg
    41101 actgcgtgac gcggctgatt caggacgacg ttaacgaaac ggcctacaac cagattaaaa
    41161 actggagcat cagcgaactg cgtgagtatg tgctgagcga tgaaaccagc gtggatgaca
    41221 ttgcctttac ccgcaaaggg ctgacctcgg aagtggtggc ggcggtagcg aagatttgct
    41281 ccaacgcgga cctgatctac ggcgcgaaga aaatgccggt gatcaaaaag gccaatacca
    41341 ccatcggtat tccgggcacc ttcagcgccc gcttgcagcc gaacgatacc cgtgacgacg
    41401 tgcaaagtat cgccgcgcaa atctacgaag ggctttcctt cggggtgggc gatgcggtga
    41461 tcggcgttaa cccggtgact gacgacgtgg aaaacttaag ccgcgtgttg gataccatct
    41521 atggcgtgat cgacaaattc aacatcccaa ctcagggctg catactggcg cacgtcacta
    41581 cccaaatcga agcgattcgt cgcggcgcac caggcgggct gattttccag agtatctgcg
    41641 gcagcgaaaa agggctgaaa gagtttggcg tagagctggc gatgctcgat gaagcacgcg
    41701 cagtgggcgc ggagttcaac cgtatcgccg gggaaaactg cctctacttc gaaaccggac
    41761 aaggttcggc gctgtctgct ggcgcgaact tcggcgcaga ccaggtgacg atggaagcgc
    41821 gtaactatgg gctggcgcgt cattacgatc cgtttatcgt caacactgtg gtcggcttta
    41881 ttgggccgga gtatctctac aacgaccgcc agattatccg cgcaggctta gaagatcact
    41941 ttatgggcaa actgagcggc atctctatgg gctgtgactg ctgctacacc aaccacgctg
    42001 acgctgacca gaacctcaac gaaaacctga tgatcctgct cgccaccgca ggctgcaact
    42061 acatcatggg gatgccgctg ggcgatgaca tcatgctcaa ctatcagacc accgcattcc
    42121 acgacactgc cactgtgcgt cagttactga atctgcgccc gtcaccggag tttgaacgct
    42181 ggctggaaag catgggcatt atggcaaacg gtcgcctgac caaacgggcg ggcgatccgt
    42241 cactgttctt ctgatgacgc ggagataaca catcatggat caaaaacaga ttgaagaaat
    42301 tgtacgcagc gtgatggcgt caatgggaca agcggccccc gcgccgtcag aagcaaagtg
    42361 cgccaccacc acctgtgcgg caccggtgac ctcggaaagc tgcgcgctgg atttaggttc
    42421 tgcggaagca aaagcgtgga ttggtgttga aaacccgcat cacgcagacg tattaacaga
    42481 actgcgtcgc agcaccgtgg cccgcgtttg taccggtcgt gccggtccgc gtccgcgcac
    42541 ccaggcgttg ctgcgtttcc tggccgatca ctctcgttcg aaagataccg ttctgaaaga
    42601 agtaccggaa gagtgggtga aagcgcaggg cttgctggaa gtccgctccg aaatcagcga
    42661 caaaaatctc tacctgactc gcccggatat gggccgccgc ctgtgtgcag aagctgttga
    42721 agcgctgaaa gcacagtgtg ttgctaatcc ggacgtgcag gtcgttattt ctgatggcct
    42781 gtcaactgat gcgatcaccg tgaactacga agagatcctg ccgccgctga tggcgggcct
    42841 gaagcaagcc gggctgaaag tcggtacgcc attctttgta cgttatggtc gcgtgaagat
    42901 tgaagatcag atcggtgaga tcctcggcgc gaaagtagtg atcctgctgg tgggcgaacg
    42961 tccggggttg gggcagtcag aaagcctctc ctgctacgcc gtttactcgc cgcgtatggc
    43021 gaccaccgtt gaggctgacc gcacttgtat ctctaacatt caccagggcg gcacgccgcc
    43081 ggttgaagcc gcagccgtca ttgtggattt ggcgaaacgt atgctggagc agaaagcatc
    43141 cggcatcaac atgacccgat aaggaggcat catgccagct ttagatttga ttcgaccgtc
    43201 ggtaaccgcc atgcgggtga ttgcctccgt taacgccgaa tttgcacgtg aactgaaatt
    43261 gccgccgcat attcgtagcc tcgggctgat ttctgctgat tcagatgacg tcacctacat
    43321 tgcggctgac gaagcgacca agcaggcgat ggtcgaagtg gtatatggtc gctcgctgta
    43381 tgcgggcgct gcacacggcc cgtcaccgac tgccggtgag gtgctgatta tgcttggtgg
    43441 accaaacccg gcggaagtgc gtgctggtct ggatgcgatg gttgcgaata ttgaaaatgg
    43501 cgctgctttc cagtgggcta atgacgcaga aaacacagcc ttcctggcac atgtagtttc
    43561 gcgtaccggt tcttatctct catcaaccgc cgggatcacg cttggcgatc cgatggcgta
    43621 tctggtggca ccgccgctgg aagcgaccta cggcattgat gcagcgttga aatctgccga
    43681 cgtgcagctg gtgacctatg tcccgccacc gtctgaaacc aactactcgg cagcattttt
    43741 aaccggtagc caggccgcgt gtaaagcagc ctgtaacgcc tttaccgatg cagtgctgga
    43801 aatcgcgcgt aatccaatcc agcgtgcgta acggaggttg ccgatgatca atgcactggg
    43861 attgctggaa gtggacggca tggtcgccgc gatagatgca gctgatgcca tgctcaaagc
    43921 agctaacgtt cgtctgctca gtcacgaagt gcttgaccct ggtcgcctaa cgctggtggt
    43981 ggaaggcgat ctggcggcgt gtcgtgcggc gctggacgca ggttgtgctg ccgcgatgcg
    44041 taccgggcgt gtcatcagcc gcaaggagat cggtcggcca gacgatgaca cccagtggct
    44101 ggttactggc tttaaccgcc agcctaagca acccgtaaag gaacccgacg cgccagttat
    44161 cgtcgcggaa tctgctgacg agttgttggc gctgttaaca tcagtacgtc agggaatgac
    44221 ggcaggagaa gtggctgccc actttggctg gccgctggaa aaagccagaa atgcgctcga
    44281 acagctcttt tctgccggga cgttacgtaa acgcagtagt cgttaccgtc tcaagcccca
    44341 ttaacctgtc ggaggtgccg ggtgtcctac aacacccggc attaacatca tgaaaaagac
    44401 ccgtacagcc aatttgcacc atctttatca tgaaccctta cccgaaaacc tgaagctcac
    44461 gccgaaggtc gaagtggata atgttcatca acgacagaca acggacgtct atgaacatgc
    44521 tttgacaatt accgcctggc agcagattta cgatcagctg catccgggca agtttcatgg
    44581 tgaatttacg gaaattctac tcgatgatat tcaggttttt cgtgaataca ctggtctggc
    44641 gctgcgtcag tcgtgcctgg tctggccgaa ctcgttctgg tttggcattc cggcgacgcg
    44701 cggtgagctg ggatttatcg gttcgcaatg tctgggaagc gcagaaattg cgacgcgccc
    44761 gggtggaacc gagtttgaat taagtacgcc ggacgattac acgatccttg gcgtagtgct
    44821 ttctgaagat gtcatcactc gtcaggctaa ctttttgcat aatccggatc gggtattaca
    44881 tatgctgcgt agccagtcgg cgctggaagt gaaagagcag cataaagccg ctctgtgggg
    44941 ctttgtccaa caggcgctgg cgacgttttg cgagaacccg gaaaatctcc atcagcctgc
    45001 agtgcgaaaa gtgctggggg ataatttgct catggcgatg ggggcgatgc tggaagaagc
    45061 gcagccgatg atgacggcgg aaagcatcag ccatcagagt taccgccgat tgctttcccg
    45121 cgcccgtgaa tatgtgctgg aaaacatgtc cgaaccggtg acggtgctgg acttgtgtaa
    45181 tcaactgcat gtcagccgcc gcacgctaca aaacgcattt cacgccattt tgggcattgg
    45241 tccgaacgcg tggctgaaac gtattcgcct gaacgccgtg cgccgcgaac tcataagtcc
    45301 gtggtcacaa agcacaacgg taaaagacgc cgccatgcag tggggattct ggcatcttgg
    45361 gcaatttgcc acggattatc agcaactgtt tgccgagaag ccgtcactga cgctgcatca
    45421 gcggatgcga gagtgggggt gagggagtta cacccaatct ctgaccttaa taaactcact
    45481 taaagccgct tccgggctgc cgtcttttgg ctgataatca tactcccagc gtaccagtgg
    45541 cggcattgac atcaggatcg actcggtgcg cccgccagtt tgcaggccaa acagcgtgcc
    45601 gcgatcccag accagattga actcgacata acgaccgcga cggtagagct gaaaattgcg
    45661 ctcgcgctcg ccgtaggcca tcgctttccg tcgctctaca attggtaaat aagcgtcggt
    45721 gtagcctttg cctaccgcct gcataaaggc aaaacagtgg tcgaaatctg gcgtgttcag
    45781 atcatcaaag aacagcccgc caatgccgcg ctgttcgttg cgatgtttga ggtagaagta
    45841 ttcgtcgcac cattttttgt aacgcggata aacgtcttcg ccaaatggca ggcacaggtc
    45901 acgggcggtg cggtgccagt gaatggcgtc ttcttcaaaa ccatagaaag gggttaaatc
    45961 gaagccgccg ccaaaccacc agacgggctc tgcacccggt ttttcggcaa taaaaaagcg
    46021 cacattcgcg tggctggtgg gaacatacgg attatgcgga tgcactacca gtgaaacgcc
    46081 catcgcctcg aaactgcgcc cggcaagttc cggacgatga gcggtggcgg aagcaggcat
    46141 cgcctcaccg tgaacatgtg aaaagttgac gcccgcttgt tcgaaaacac caccattacg
    46201 caacacccga ctacgcccac cgccgccagc ttcacgttgc cagctatctt cgacaaactc
    46261 tgcgccatcg acggcggtca gttgttgaca aattgtatcc tgaaggttga gcaagaactg
    46321 tttaaccagg tgtgcgtcgg gtttcatcac ttaccgcttt ctcgagtgtg ctttctggtt
    46381 gtcaaaccag tggaaataac tgatcacgcc ttcggcaatc gctgtggcga ttttctggcg
    46441 aaacgccgcc gtgcctaaca gccgctcttc ttccgggttg gtgataaacg aggtttccac
    46501 cagcaccgac ggaaccgacg gcgatttcaa caccacaaac gcggcttgtt cggtgttgcg
    46561 gctgtgcagt ttatgcaccg gcttaatctt tttcagaata tgcgagccga gcgtcaggct
    46621 gtttttaata gtatcggttt gcaccagatc aaacagcact tgctgcaata ggtgatcctt
    46681 gtcagtcgct tttttaccgg caacttcatc ggcgcggttt tcgcgttcag acaggtattt
    46741 tgccattgcg ctacttgccc cgcggttaga gagggcaaat accgaagcac cggcagcttt
    46801 cgggttggta aagccgtcgg catgaattga cataaacaga tctgcgccat gtttatgggc
    46861 gatttcaacg cgatcgtaca gtgggataaa cgtatcgcca gaacgcgtta aacgcgcatc
    46921 aatgccatga ttacgcaata tggaacggac attcttggca attgccagca ccacatgttt
    46981 ttctttcgaa ccgttgcgtc cgatcgctcc ggtatcaatc ccgccgtggc ctggatcgag
    47041 aacaacgaca cgtctgccgc cgcttttttt tgctttcggc ttgctgtgtc cgttactggt
    47101 ttttaacggt tcttctttgg cgatggcttg cgacattcct gacaacgtca gggcagccaa
    47161 accggctttc agcacctggc ggcgcgaagt gagtgttttt agtggtttaa aagtgctcat
    47221 acggcctgag ttgtaataat aaagttccag atgttatatc gtatcgcgta atccgttacg
    47281 acggtttgct gttgagaatt gttttacttt tcatttcaat agttgacaac tccccattat
    47341 gccacttttt caaccgattt tcgtcagata ttggctaatt cgaccgttca cgccataatc
    47401 agccttttaa cccgcaaaaa ggtaacgaat accatggaga tacgcgtatt tcgccaggaa
    47461 gatttcgaag aggtcatcac cctttgggag cgttgcgact tgctgagtcc gtggaacgat
    47521 ccggaaatgg acatcgagcg taagatgaac catgacgtca gtttgtttct ggtcgctgag
    47581 gtgaacggtg aagtggtcgg aacggtgatg ggcggttatg acgggcatcg cgggtctgct
    47641 tattatcttg gcgtgcatcc cgagtttcgt gggcgtggga ttgctaatgc gttgcttaat
    47701 cggctggaga aaaagctgat tgctcgcggc tgtccgaaaa ttcagatcaa cgtgccggaa
    47761 gataacgaca tggtgctcgg aatgtatgaa cgcctgggat atgaacacgc cgacgtgctg
    47821 agtctgggta agcgtttgat tgaagatgaa gagtactgag tttcatcctg tccattatga
    47881 tgcgcacggt cgcctgcgtt tacccttgct cttctggctt gtgctattgc ttcaggcgcg
    47941 aacctgggtg ctgtttgtca ttgccggagc gtcgcgcgag cagggtactg cgctgctaaa
    48001 tctgttttat cccgatcacg ataatttctg gctggggttg attcccggta ttccggcggt
    48061 gctggcattt ttgctgagtg gtcggcgggc ttcatttccc cgtatctggc atgtgctcta
    48121 ttttctgttg ctgttggcgc aagtggtttt actttgttgg caaccgtggc tgtggctgaa
    48181 cggtgaatcc gttagcggta tcggtctggc gctggtagtg gcggatatcg tggcgttaat
    48241 ctggctgctg accaatcgac gtttacgcgc ttgttttaat gaagaaaaag aataacggca
    48301 ctttttggtg aatttgcact ccaagcaacg ttattgaata accaaaggaa gtgacatgaa
    48361 atcgctgcgt ttaatgttat gcgctatgcc gttgatgctg accggctgtt ccacgatgtc
    48421 gtcagttaac tggtcggccg ctaacccgtg gaactggttt ggatcatcca ccaaagtgag
    48481 cgagcagggc gtgggtgaat taacggcgtc cacaccactg caagaacaag ccattgctga
    48541 tgcgcttgat ggcgattatc gcctgcgcag cggaatgaaa accgcgaacg gcaacgtggt
    48601 gcgctttttt gaagtgatga aaggcaacaa cgtggcgatg gtgattaacg gcgatcaagg
    48661 cacgatcagc cgcattgatg tgctggatag cgatattcct gctgacaccg gtgttaaaat
    48721 cggtacaccg tttagtgacc tttacagcaa agcatttggc aattgccaaa aggccgacag
    48781 tgatgataat cgtgccgtcg aatgtaaagc cgaaggcagt caacatatta gctaccagtt
    48841 cagcggggaa tggagtggtc ctgaagggtt aatgccttcg gacgataccc tgaaaaactg
    48901 gaaagtcagt aaaattatct ggcggcgtta atttgcgtct gaacaaaccg ccgccgcgaa
    48961 aaaacacgta aaatagcgcc caacaatgcc acggattgcg tggcattatt attttcagga
    49021 ggaacaatgt ctcaggttca gagtggcatt ttgccagaac attgccgcgc ggcgatttgg
    49081 atcgaagcca acgtgaaagg ggaagttgac gccctgcgtg cggccagtaa aacatttgcc
    49141 gacaaactgg caacttttga agcgaaattc ccggacgcgc atcttggtgc ggtggttgcc
    49201 tttggtaaca atacctggcg cgctctgagc ggcggcgttg gggccgaaga gctgaaagat
    49261 tttccgggct acggtaaagg tcttgcaccg acgacccagt tcgatgtgtt gatccacatt
    49321 ctttctctgc gtcacgacgt gaacttctct gtcgctcagg cggcgatgga agcctttggt
    49381 gactgcattg aagtgaaaga agagatccat ggcttccgtt gggttgaaga gcgtgacctg
    49441 agcggctttg ttgacggtac agaaaacccg gcgggtgaag agacgcgccg cgaagtggct
    49501 gttatcaaag acggcgtgga tgcgggcggc agctatgtgt ttgtgcagcg ttgggagcac
    49561 aacctgaagc agctcaatcg gatgagcgtt cacgatcaag agatgatgat tgggcgcacc
    49621 aaagaggcca acgaagagat tgacggcgac gaacgtccgg aaacctctca cctgacccgc
    49681 gttgatctga aagaagatgg caaagggctg aagattgttc gtcagagcct gccgtacggc
    49741 accgccagtg gcactcacgg tctgtacttc tgcgcctact gcgcgcgtct gcataacatt
    49801 gagcagcaac tgctgagcat gtttggcgat accgatggta agcgtgatgc gatgttgcgt
    49861 ttcaccaaac cggtcactgg cggctattac ttcgcaccgt cgctggacaa gctgatggcg
    49921 ctgtaagcgt ttctgattca ttgattaaag gccagccagt aacacaactg actggccttt
    49981 ttattactgc tttgacgccg catacacttg atcaactact ttctgccaaa acagggtatt
    50041 ttgcggcgct gacgctacag ctaatagcca gcagagaaag atagagcatt gtccgtttca
    50101 taatcagtcc agattgacgt tacggcagcc aaagagtgtg gtgaaaataa acccactaac
    50161 ccatgccacc agaataccgc cagcgtaaac cgccattgcc ggaaggatgc cttgtgcgga
    50221 agtcatcagc ggcagcgcca ccagaccaga cggcccgaag gcgctgttta agcccatcgg
    50281 cagaccccac caggctatca ggccgataaa caaaccgccc gccgcgccgc ctaaacaggc
    50341 ggtaacaaac ggtttcatgc ggggcagggt gacaccgtaa atcagcggtt cgccaacgcc
    50401 aagcagaccg ggaataatcg ccccgcggac ctgactgcgt aatgcactgt gcggttgcgc
    50461 ccgccagtag agcgccagcg ccgcgcccac ctggcctgcg cctgccattg aaaggatggg
    50521 aaataagctg ttgaatccct ggctgtccat taacgccaga taaacaggaa taaagccctg
    50581 atgcacgcca aataccacgg cgatcaggaa cagcccggct aaaaccgcac aaccgagcgg
    50641 attactgttc aggtgcataa acagccatga cataccttca aacagccagc cgcccagcgg
    50701 cataatgatc aggtacgcaa gcgtagcggt gatcagcagg gtgattaatg aggtcaacag
    50761 catgtcgagg tcgtccggca taaagcggcg caccatgcat tcgatgcggg cgcaggccca
    50821 tgcggcaatc aacacgccga taatattgcc gcgcggatcg ataggcagac caaagaaatc
    50881 gtgaaatccg gcgtagtaac ccgtggttgc agcggggtta taaccgagca aaaagagcgc
    50941 agcgataatt gcgccattta cgcccgtgcc accaaatgcc tgggccgcgt tatagcccac
    51001 cagaattacc aggaaggtga acaggccttt gctgaacacc ttcataaaat tcagcgcatc
    51061 gggtagcgtg ccctgagcat ctgccggaac gtgcatcacc gtggcaatta acgtcgctat
    51121 ccccagcagc agaccggcgg caataaaacc ggggatcagc ggcgtgaaga tggtggcgaa
    51181 tttggcgaga aattgttgca cgccggaagt ttgtctggct tttaactggc gtttattctg
    51241 ggcggcgatt tctgcggcat cctgtaccgg tgcctcgccc agcagctcgc tcatggcttt
    51301 tgcggcacga tgcgctttgc ctggcccaaa aaccacctgc acctgatcgc tggtcaaaat
    51361 gacccccttc acgccttcca gcgttttgat attgggatca accagtgaac tgtcatgtac
    51421 acccagacgc aggcgcgtca tacagttacc acaactggcg atatttcccg gtccgccgac
    51481 ccgggtaaga atggtgttta gaagttcact gctgatctct ttagccatgg gttattcctt
    51541 ttccagagcc ttgcgaataa aaccgccgtg ttgatccagg cattttttag cttcagcggc
    51601 atcgagattt ttcagtacca tcactatggc cgttttacag ttgcgctcgc aagcaattaa
    51661 cgccgcttcc gcttgttcgg cattacatcc ggtagcgttt ttgacgatat tgacctggcg
    51721 cacatgcagt ttttcgttgg tggcgaccac atcgaccatc aggttgccga acactttgcc
    51781 ggatttaatc atcagcccgg tagagagcat gttgagcacc agtttctgcg ccgtgcctgc
    51841 tttcattcgc gaagaaccgg taacaacttc ggcaccaact accggcgtaa tagcaaactc
    51901 tgcggtggat gaaacggcac tccccggatt acaggaaatg cccactgtac ggcagccgag
    51961 ttggcgcgcg tattccagcc cggcaatcac atacggcgtg cgtccgctgg ccgcaatacc
    52021 aaccaccaca tcctgcgcgg ttaagccgat atttttcaga tcattaacac caccttcccg
    52081 gctatcttct gcgccttcca ccgcgtgctg aatggcatat tcgccgccag caatcaaacc
    52141 aactaccagg cccggtttca cgccgtaggt cggcggacat tcgctggcat ccagaatccc
    52201 cagacgaccg gatgttcccg caccgagata aatcagacgg ccgccgccgc ttacctgggt
    52261 gtggataaca tcgatcgccg cggcgatatc cggcagtacg cgctcaacgg cgagtggtac
    52321 ggttttatct tcatcgttga taatccggca catttccagc gttgatacgc ggtcaatttc
    52381 agccgaggcg gcgttcgagc cttcggtaat catcttttca agttgcatcg gtggtgacct
    52441 taatatttgc gatcaaataa ttgcagataa atgggatcat atatcccgat ttgatagaca
    52501 caaaggaatc attaagtccg aatattactc atgtgatgtc actgaaaatg attgcgcgct
    52561 gacgtaacgc ttgaggtgct gcataaagca gcgactaaat tgagattttc ctgaattagt
    52621 gagctgatcc gcagcaatat tttgtttatc ctgtattttc agagggaatg gagtgtaacg
    52681 ctctgtatta acaaggagag cattaaaatg ggtaaactca cgggcaagac agcactgatt
    52741 acgggcgcat tgcagggaat tggcgaagga attgccagaa cttttgcacg tcatggcgca
    52801 aacctaatct tgctggatat ctcccctgag atcgaaaagc tggcggacga actgtgtggt
    52861 cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg acccggcgtc ggtagccgca
    52921 gctattaagc gcgctaagga aaaagaagga cgcattgata ttctggtgaa taacgcaggc
    52981 gtttgtcgtc tgggcagctt cctcgatatg agcgatgaag atcgcgattt ccatattgat
    53041 atcaatataa aaggcgtatg gaacgtcacg aaggcggtgc tgccggagat gattgcccgc
    53101 aaagatggcc gcattgtgat gatgtcttca gtcactggcg atatggtggc agacccgggc
    53161 gaaacggcgt acgccttaac gaaagcggcg attgttggcc tgactaaatc gctggcggtg
    53221 gagtacgcgc agtctggtat tcgcgttaac gccatctgcc ctggatacgt tcgcacgcca
    53281 atggcggaaa gcattgcccg ccagtcgaac ccggaagatc cagaatcggt gctgactgaa
    53341 atggcgaaag caatcccgat gcgtcgcctc gccgatccgc tggaagtcgg cgaactggcg
    53401 gccttcctcg catcggatga atccagctat ttaacaggta cacagaatgt gattgatggc
    53461 ggcagcacac tgccggagac ggttagcgtc ggcatctgat tcacctctgt ttcctccctg
    53521 catttgtggg gaggatttcg tcttattccc ttttcaactt ccaaatcacc aaacggtata
    53581 taaaaccgtt actcctttca cgtccgttat aaatatgatg gctattagaa agtcattaaa
    53641 tttataaggg tgcgcaatgg ccgttaactt actgaaaaag aactcactcg tgctggtcgc
    53701 ttctctgctg ctggcgggcc atgtacaggc aacggaactg ctgaacagtt cttatgacgt
    53761 ctcgcgcgag ctgtttgccg ccctgaaccc gccgtttgaa cagcaatggg ccaaagataa
    53821 cggcggcgac aaactgacga taaaacaatc tcatgccggg tcatcaaaac aggcgctggc
    53881 gattttgcag ggcttaaaag ccgacgttgt cacttataac caggtgacag acgtgcagat
    53941 cctgcacgac aaaggcaagc ttattccggc tgactggcag tcgcgtctgc caaacaacag
    54001 ctcgccgttc tactccacca tgggcttcct ggtgcgtaag ggtaacccga agaatatcca
    54061 cgactggaac gatctggtgc gctccgacgt gaagctgatt tttccgaacc cgaaaacctc
    54121 cggtaacgcg cgttacacct atttagccgc gtggggtgca gcggataaag ccgacggtgg
    54181 cgataaagcc aaaaccgaac agtttatgac ccagttcctg aaaaacgttg aagtgttcga
    54241 tactggcggt cgtggcgcga ccaccacttt tgccgagcgc ggcctgggcg atgtgctgat
    54301 cagcttcgag tcggaagtga acaacatccg taaacagtat gaagcgcagg gctttgaagt
    54361 ggtgattccg aaaaccaaca ttctggcgga attcccggtg gcgtgggtgg ataaaaacgt
    54421 acaggccaac ggcacggaaa aagcggcaaa agcctacctg aactggctct acagcccgca
    54481 ggcgcaaacc atcatcaccg actattacta ccgcgtaaat aacccggaag tcatgggcaa
    54541 actgaaagat aaattcccgc agaccgagct gttccgcgtg gaagataaat ttggctcctg
    54601 gccggaagtg atgaaaaccc acttcaccag cggcggcgag ttagacaagc tgttagcggc
    54661 ggggcgtaag taatgtttgc agtttcttca agacgcgtgc tgccgggctt taccttaagc
    54721 ctcggcacca gtctgctgtt tgtgtgcctg attttgctgc tgccgctctc cgcgctggtg
    54781 atgcaactgg cccagatgag ctgggcgcag tactgggagg tgatcaccaa cccgcaggtg
    54841 gtcgcagcct ataaagtgac gctgctgtcg gcgtttgtgg catcgatttt taacggcgtt
    54901 ttcggtctgc tgatggcgtg gatcttaacc cgctatcgct ttccgggtcg cacgctgctt
    54961 gatgcgctga tggatttacc ctttgcgctg ccaacggcag tcgccggttt aacgctggcc
    55021 tcgctctttt ccgtgaacgg tttttacggt gaatggctgg cgaagtttga tatcaaagtc
    55081 acctatacct ggctggggat tgcggtggcg atggccttta ccagcattcc gtttgtggtg
    55141 cgcaccgtgc agccggtgct ggaagagtta ggcccggaat atgaagaggc ggcagaaacg
    55201 ctcggcgcaa cacgctggca gagttttcgc aaagtggtgc tgccggaact atctccggca
    55261 ctggtggcgg gtgtggcgct gtcgtttacc cgcagcctcg gtgagtttgg cgcggtgatt
    55321 tttatcgccg ggaatatcgc ctggaaaaca gaagtgacgt cgctgatgat ctttgttcgc
    55381 ttacaggagt ttgattaccc ggcggcgagc gcgattgcct cggtgatcct cgcggcatcg
    55441 ctgctgttgc tgttctcaat taacactctg caaagtcgct ttggtcggcg tgtggtaggt
    55501 cattaatggc ggaagtgaca caattgaagc gttatgacgc gcgcccgatt aactggggca
    55561 aatggtttct gattggcatc gggatgctgg tatcggcgtt catcctgctg gtgccgatga
    55621 tttatatctt cgtacaggcg ttcagcaaag gactgatgcc ggtgttacaa aatctggccg
    55681 atccggacat gctgcacgcc atctggctga cggtgatgat cgcgctgatt gccgtaccgg
    55741 taaacctggt gttcggcatt ctgctggcct ggctggtgac gcgctttaac ttccccggac
    55801 gccagctatt gctgacgctg ctggacattc cgtttgccgt gtcgccggtg gttgctggtc
    55861 tggtgtatct gctgttctac ggctctaacg gcccgctggg cggctggctg gatgaacata
    55921 atctgcaaat tatgttctcc tggccgggta tggtgctggt caccatcttc gtgacgtgtc
    55981 cgtttgtggt gcgcgaactg gtaccggtaa tgttaagtca gggcagccag gaagacgaag
    56041 cggcgatttt acttggcgcg tccggctggc agatgttccg tcgcgtcacg ctgccgaaca
    56101 tccgctgggc gctgctttac ggcgtggtgc tgaccaacgc ccgcgcgatt ggcgagtttg
    56161 gcgcggtatc ggtggtttcc ggctcgattc gtggcgaaac cctgtcgctg ccgttacaga
    56221 ttgaattgct ggagcaggac tacaacaccg tcggctcctt taccgccgcc gcgttgttga
    56281 cgctgatggc gattatcacc ctgtttttaa agagtatgtt gcagtggcgc ctggaaaatc
    56341 aggaaaaacg cgcgcagcag gaggaacatc atgagcattg agattgccaa tattaagaag
    56401 tcttttggtc gcacccaggt gctgaacgat atctcactgg atattccttc tggtcagatg
    56461 gtcgcgttgc ttgggccgtc cggttccgga aaaactacgc tgctgcgcat tatcgccggg
    56521 ctggagcatc aaaccagtgg gcatattcgc tttcacggta ccgacgtgag ccgtctgcac
    56581 gcgcgggacc gtaaagtcgg tttcgtgttc cagcattacg cgctgtttcg ccatatgacg
    56641 gtgtttgaca atatcgcttt tggcctgacg gtgttgccac ggcgtgaacg cccgaatgcg
    56701 gcggcgatca aagcgaaagt gacaaaattg ctggagatgg tgcagcttgc tcatctggcg
    56761 gatcgttatc cggcgcagct ttccggcggt cagaaacagc gtgtggcgct tgcacgcgcg
    56821 ctggctgtgg aaccgcaaat tttgctgctt gatgaaccgt ttggcgcgct ggatgcgcag
    56881 gtgcgtaaag agctgcgtcg ctggctgcgt caactgcatg aagaactgaa attcaccagc
    56941 gtgttcgtga cccacgacca ggaagaggcg accgaagtag ctgatcgtgt agtggtgatg
    57001 agtcagggca atattgaaca agctgacgca ccggatcagg tatggcgtga accggcgact
    57061 cgttttgtgc tggagtttat gggcgaggta aaccgcctgc agggaaccat tcgcggcggg
    57121 cagttccacg ttggcgcaca tcgctggccg ctgggataca cccccgcgta tcaggggccg
    57181 gtggatctct tcctgcgccc gtgggaagtg gatatcagcc gccgtaccag cctcgattcg
    57241 ccgctacccg tacaggtact ggaagccagc ccgaaaggtc actacaccca attagtggtg
    57301 cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga tgcatggcga cgatgccccg
    57361 cagcgtggcg atcgtttata cgttggtctg caacatgcgc ggctgtataa cggcgatgag
    57421 cgtattgaaa cccgcgatga ggaacttgct ctcgcacaaa gcgcctgata ggttgagtga
    57481 atgttaaacg cccggaggcg cttcccgcga tccgggtttt ttaatggcaa ggttttgtaa
    57541 cctgtaggtc tgataagacg cgtaagcgtc gcatcaggca acaccacgta tggacagaga
    57601 tcgtgagtac attagaacaa acaataggca atacgcctct ggtgaagttg cagcgaatgg
    57661 cgccggataa cggcagtgaa gtgtggttaa aactggaagg caataacccg gcgggatcgg
    57721 taaaagaccg tgcggcgctt tcgatgattg tcgaggcgga aaagcgcggc gagattaaac
    57781 cgggtgatgt gctgatcgaa gccaccagtg gtaacaccgg cattgcgctg gcaatgattg
    57841 cggcgcttaa aggctatcgc atgaaattgc tgatgcccga caacatgagc caggaacgcc
    57901 gtgcggcgat gcgtgcctac ggtgcagaac tgattctggt caccaaagaa cagggcatgg
    57961 aaggcgcacg cgatctggcg ctggagatgg cgaatcgcgg cgaaggaaag ttgctcgatc
    58021 agttcaataa tcccgataac ccttacgccc attacaccac taccgggccg gaaatctggc
    58081 agcaaactgg cgggcgtatt acccattttg tctccagcat gggaacaacc ggcactatca
    58141 ccggcgtgtc acgctttatg cgcgaacaat ccaaaccggt gaccattgtt ggcctgcagc
    58201 cggaagaagg cagcagtatt cccggcattc gccgctggcc tgcggaatat ctgccgggga
    58261 ttttcaatgc ttctctggtg gatgaggtgc tggatattca tcagcgcgat gcggaaaaca
    58321 ccatgcgcga actggcagtg cgggaaggga ttttctgcgg cgtaagctcc ggcggcgcgg
    58381 ttgccggagc actgcgggtg gcaaaagcta accctggcgc ggtggtggtg gcgatcatct
    58441 gcgatcgtgg cgatcgctac ctttctaccg gggtgtttgg ggaagagcat tttagccagg
    58501 gggcggggat ttaagattat tgcgcaggca ccgttttatc cgtctgcgca attaacgcat
    58561 gtaaaaaagg ctgtgcgttc tcatcgctct taccgggcat cttcacgata tacggcttcc
    58621 cggtaatcga tgacgacgag gccaccttgt caataaactg ctcggctgta tcaatgcgat
    58681 tacgggtatt gcccagcttc aggcgcagat gagaaaccgc ttcatcgcag gtatgttcat
    58741 caccgttgcg cacaaatatc aaatcctttt tctgtgctaa tccctccagc atggcgttga
    58801 tacgggcttc ttcatgggcc gttaacttcg cataagcggg gagtgtcatt agcagcgtaa
    58861 cgaccagaca aatgattttc ttcactgaat taccttatcc ttgttttgta atagccgatt
    58921 tgactgccag aatcaggtaa agtttcggct ggaatacgat gaggatcatc ggcaaggaga
    58981 aaaaattaaa tgagtagttt gttgttgttt aacgataaga gcagggcact gcaggcggat
    59041 atcgtcgccg tgcagtcgca ggtagtgtac ggcagcgtag gcaacagcat tgcggtgcct
    59101 gctataaagc agaacggcct gaatgtcttt gccgtgccga cggtattgct gagcaatacg
    59161 ccgcattatg acactttcta cggtggtgcg attccggatg aatggtttag cggttatttg
    59221 cgtgcgcttc aggagcgtga tgcgctgcgc caacttcgtg ctgtaaccac gggctatatg
    59281 ggaacggcat cgcaaatcaa aatccttgcc gagtggctga ctgcgctacg caaagaccat
    59341 cctgacctat tgatcatggt cgatccggtg attggcgata ttgatagcgg aatttatgtc
    59401 aaacctgacc ttcccgaggc gtatcgacaa tatttactgc cgctggcaca gggaattacc
    59461 cccaatatct ttgagctgga aatcctgacc ggtaaagatt gccgcgatct cgacagtgcc
    59521 attgctgccg caaaaagtct gctttcagac acattaaagt gggtggtgat taccagcgcc
    59581 tccggcaatg aagaaaatca ggagatgcag gttgtggtgg tcagtgccga cagcgtgaat
    59641 gtcatttccc attcccgggt taaaactgac ctgaaaggga ctggcgacct gttttgtgct
    59701 cagcttatca gtggtttgct gaaagggaag gcgttaaccg atgcggtgca ccgcgcgggg
    59761 ttgcgcgtac tggaagtgat gcgctatacc cagcagcatg agagcgatga attgattttg
    59821 ccgcctc
//
LOCUS       DAOFSV010000011        59314 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.011, whole genome shotgun sequence.
ACCESSION   DAOFSV010000011 DAOFSV010000000
VERSION     DAOFSV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.011"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(199..675)
                     /locus_tag="QBH27_000777"
     CDS             complement(199..675)
                     /locus_tag="QBH27_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDQ7013134.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(763..1665)
                     /gene="rimK"
                     /locus_tag="QBH27_000778"
     CDS             complement(763..1665)
                     /gene="rimK"
                     /locus_tag="QBH27_000778"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDQ7013135.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(1726..2448)
                     /gene="nfsA"
                     /locus_tag="QBH27_000779"
     CDS             complement(1726..2448)
                     /gene="nfsA"
                     /locus_tag="QBH27_000779"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDQ7013136.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(2432..2719)
                     /locus_tag="QBH27_000780"
     CDS             complement(2432..2719)
                     /locus_tag="QBH27_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDQ7013137.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            2879..3136
                     /gene="grxA"
                     /locus_tag="QBH27_000781"
     CDS             2879..3136
                     /gene="grxA"
                     /locus_tag="QBH27_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDQ7013138.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(3166..3543)
                     /locus_tag="QBH27_000782"
     CDS             complement(3166..3543)
                     /locus_tag="QBH27_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDQ7013139.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            3812..5497
                     /locus_tag="QBH27_000783"
     CDS             3812..5497
                     /locus_tag="QBH27_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDQ7013140.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(5673..6209)
                     /gene="rcdA"
                     /locus_tag="QBH27_000784"
     CDS             complement(5673..6209)
                     /gene="rcdA"
                     /locus_tag="QBH27_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDQ7013141.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            6293..7501
                     /locus_tag="QBH27_000785"
     CDS             6293..7501
                     /locus_tag="QBH27_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7013142.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMVLSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATMHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMMVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            7501..8316
                     /gene="ybjI"
                     /locus_tag="QBH27_000786"
     CDS             7501..8316
                     /gene="ybjI"
                     /locus_tag="QBH27_000786"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDQ7013143.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            8402..8686
                     /locus_tag="QBH27_000787"
     CDS             8402..8686
                     /locus_tag="QBH27_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013144.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(8727..9959)
                     /gene="mdfA"
                     /locus_tag="QBH27_000788"
     CDS             complement(8727..9959)
                     /gene="mdfA"
                     /locus_tag="QBH27_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDQ7013145.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            10244..10840
                     /gene="ybjG"
                     /locus_tag="QBH27_000789"
     CDS             10244..10840
                     /gene="ybjG"
                     /locus_tag="QBH27_000789"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDQ7013146.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10898..11656
                     /gene="deoR"
                     /locus_tag="QBH27_000790"
     CDS             10898..11656
                     /gene="deoR"
                     /locus_tag="QBH27_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDQ7013147.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHISKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(11703..12905)
                     /gene="dacC"
                     /locus_tag="QBH27_000791"
     CDS             complement(11703..12905)
                     /gene="dacC"
                     /locus_tag="QBH27_000791"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDQ7013148.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            13152..13778
                     /gene="gstB"
                     /locus_tag="QBH27_000792"
     CDS             13152..13778
                     /gene="gstB"
                     /locus_tag="QBH27_000792"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDQ7013149.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDTDF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(13775..14890)
                     /gene="yliI"
                     /locus_tag="QBH27_000793"
     CDS             complement(13775..14890)
                     /gene="yliI"
                     /locus_tag="QBH27_000793"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDQ7013150.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(15001..15384)
                     /gene="bssR"
                     /locus_tag="QBH27_000794"
     CDS             complement(15001..15384)
                     /gene="bssR"
                     /locus_tag="QBH27_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDQ7013151.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            15597..16922
                     /gene="rimO"
                     /locus_tag="QBH27_000795"
     CDS             15597..16922
                     /gene="rimO"
                     /locus_tag="QBH27_000795"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDQ7013152.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(16969..18297)
                     /locus_tag="QBH27_000796"
     CDS             complement(16969..18297)
                     /locus_tag="QBH27_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ7013153.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(18305..20653)
                     /locus_tag="QBH27_000797"
     CDS             complement(18305..20653)
                     /locus_tag="QBH27_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ7013154.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTDLVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERANKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIATVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRNH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRV
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(20830..21741)
                     /gene="gsiD"
                     /locus_tag="QBH27_000798"
     CDS             complement(20830..21741)
                     /gene="gsiD"
                     /locus_tag="QBH27_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDQ7013155.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(21744..22664)
                     /gene="gsiC"
                     /locus_tag="QBH27_000799"
     CDS             complement(21744..22664)
                     /gene="gsiC"
                     /locus_tag="QBH27_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDQ7013156.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGILLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(22682..24220)
                     /gene="gsiB"
                     /locus_tag="QBH27_000800"
     CDS             complement(22682..24220)
                     /gene="gsiB"
                     /locus_tag="QBH27_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDQ7013157.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(24240..26111)
                     /gene="gsiA"
                     /locus_tag="QBH27_000801"
     CDS             complement(24240..26111)
                     /gene="gsiA"
                     /locus_tag="QBH27_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDQ7013158.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQESSIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(26098..27063)
                     /gene="iaaA"
                     /locus_tag="QBH27_000802"
     CDS             complement(26098..27063)
                     /gene="iaaA"
                     /locus_tag="QBH27_000802"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDQ7013159.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACLMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            27267..28502
                     /gene="moeA"
                     /locus_tag="QBH27_000803"
     CDS             27267..28502
                     /gene="moeA"
                     /locus_tag="QBH27_000803"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDQ7013160.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            28502..29251
                     /gene="moeB"
                     /locus_tag="QBH27_000804"
     CDS             28502..29251
                     /gene="moeB"
                     /locus_tag="QBH27_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDQ7013161.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(29327..29989)
                     /gene="fsa"
                     /locus_tag="QBH27_000805"
     CDS             complement(29327..29989)
                     /gene="fsa"
                     /locus_tag="QBH27_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDQ7013162.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAVGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            30120..31019
                     /locus_tag="QBH27_000806"
     CDS             30120..31019
                     /locus_tag="QBH27_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDQ7013163.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31025..33457
                     /locus_tag="QBH27_000807"
     CDS             31025..33457
                     /locus_tag="QBH27_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDQ7013164.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            33603..34418
                     /gene="ybiV"
                     /locus_tag="QBH27_000808"
     CDS             33603..34418
                     /gene="ybiV"
                     /locus_tag="QBH27_000808"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDQ7013165.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHITLDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(34618..36210)
                     /locus_tag="QBH27_000809"
     CDS             complement(34618..36210)
                     /locus_tag="QBH27_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDQ7013166.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            36429..37349
                     /gene="ldtB"
                     /locus_tag="QBH27_000810"
     CDS             36429..37349
                     /gene="ldtB"
                     /locus_tag="QBH27_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ7013167.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(37407..38525)
                     /locus_tag="QBH27_000811"
     CDS             complement(37407..38525)
                     /locus_tag="QBH27_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDQ7013168.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(38522..38989)
                     /gene="mntR"
                     /locus_tag="QBH27_000812"
     CDS             complement(38522..38989)
                     /gene="mntR"
                     /locus_tag="QBH27_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDQ7013169.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            39175..39303
                     /gene="mntS"
                     /locus_tag="QBH27_000813"
     CDS             39175..39303
                     /gene="mntS"
                     /locus_tag="QBH27_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDQ7013170.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            39575..41158
                     /gene="opgE"
                     /locus_tag="QBH27_000814"
     CDS             39575..41158
                     /gene="opgE"
                     /locus_tag="QBH27_000814"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDQ7013171.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(41207..41722)
                     /gene="ompX"
                     /locus_tag="QBH27_000815"
     CDS             complement(41207..41722)
                     /gene="ompX"
                     /locus_tag="QBH27_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDQ7013172.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(41775..41840)
                     /locus_tag="QBH27_000816"
     CDS             complement(41775..41840)
                     /locus_tag="QBH27_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013173.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            42075..42962
                     /gene="rhtA"
                     /locus_tag="QBH27_000817"
     CDS             42075..42962
                     /gene="rhtA"
                     /locus_tag="QBH27_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDQ7013174.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            43262..43765
                     /gene="dps"
                     /locus_tag="QBH27_000818"
                     /gene_synonym="pexB"
     CDS             43262..43765
                     /gene="dps"
                     /locus_tag="QBH27_000818"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDQ7013175.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            44169..44915
                     /gene="glnH"
                     /locus_tag="QBH27_000819"
     CDS             44169..44915
                     /gene="glnH"
                     /locus_tag="QBH27_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDQ7013176.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            45054..45713
                     /gene="glnP"
                     /locus_tag="QBH27_000820"
     CDS             45054..45713
                     /gene="glnP"
                     /locus_tag="QBH27_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDQ7013177.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            45710..46432
                     /gene="glnQ"
                     /locus_tag="QBH27_000821"
     CDS             45710..46432
                     /gene="glnQ"
                     /locus_tag="QBH27_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDQ7013178.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            46549..48774
                     /gene="ybiO"
                     /locus_tag="QBH27_000822"
     CDS             46549..48774
                     /gene="ybiO"
                     /locus_tag="QBH27_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDQ7013179.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYCKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKTVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(48771..49697)
                     /gene="rlmF"
                     /locus_tag="QBH27_000823"
     CDS             complement(48771..49697)
                     /gene="rlmF"
                     /locus_tag="QBH27_000823"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDQ7013180.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            49973..50233
                     /locus_tag="QBH27_000824"
     CDS             49973..50233
                     /locus_tag="QBH27_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7013181.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            50498..52780
                     /locus_tag="QBH27_000825"
     CDS             50498..52780
                     /locus_tag="QBH27_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDQ7013182.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQKSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGFSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPTVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            52822..53499
                     /gene="ybiX"
                     /locus_tag="QBH27_000826"
     CDS             52822..53499
                     /gene="ybiX"
                     /locus_tag="QBH27_000826"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDQ7013183.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            53573..53839
                     /gene="ybiI"
                     /locus_tag="QBH27_000827"
     CDS             53573..53839
                     /gene="ybiI"
                     /locus_tag="QBH27_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDQ7013184.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            54104..54364
                     /locus_tag="QBH27_000828"
     CDS             54104..54364
                     /locus_tag="QBH27_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7013185.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(54502..55587)
                     /gene="hcxB"
                     /locus_tag="QBH27_000829"
     CDS             complement(54502..55587)
                     /gene="hcxB"
                     /locus_tag="QBH27_000829"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDQ7013186.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(55727..56689)
                     /gene="ybiB"
                     /locus_tag="QBH27_000830"
     CDS             complement(55727..56689)
                     /gene="ybiB"
                     /locus_tag="QBH27_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDQ7013187.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(56717..58867)
                     /gene="dinG"
                     /locus_tag="QBH27_000831"
     CDS             complement(56717..58867)
                     /gene="dinG"
                     /locus_tag="QBH27_000831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDQ7013188.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGALLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            58971..59171
                     /locus_tag="QBH27_000832"
                     /pseudo
     CDS             58971..59171
                     /locus_tag="QBH27_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
BASE COUNT        14680 a        15627 c        14492 g        14515 t
ORIGIN      
        1 ataactttag ccagggtttg agactatagc tcggattaaa aaacggtatt taacaaattt
       61 ttatgcgtat ttgctctatg tgataaagcc aaatcggcac aagagaagtg aattaatctt
      121 taataaaagg ttaaaatcag cggataattt tgcggcacgc agtcactgcg gcagcgactg
      181 cgtgctcgaa agaagttatc agtggaggaa atagttttcg gtaacgttat tttgcccttc
      241 atcgtcagtc ggtaacagca gttgatgggc attagcttca agaatgacca tcgaaatctg
      301 ctcttcgctc tgacgcacga accatgcaaa ctgctcatag gttacgccct gcataacgga
      361 taaagactgg caaaccacca gctttggcag attatcatcc tgcatatcaa gaaatgcttt
      421 cacggtcagc gaactggcat tgatggctga taaatccgcc gccagcggca atacggctga
      481 aggtcggact tccgccatgg cagaaaacag gatcgtgtta tcgatcagat cgattttagc
      541 atcaaagaca ccgtcgaaat tctgcatatg gggcagatgc agagcctgac agttatcaca
      601 ttcaaaaaaa ctcatcccca ggtcatcgag ccattgacgc aacgtatcca gaccaggaac
      661 gaccagcgat gtcataattt tgtacaacct cttccgataa aaagaccggc acagcttacg
      721 caaaaagcgc aggcaaaacc atgatcagta atgtgattgc gactaaccac ccgttttcag
      781 gcaatattct gtcgtagcgt ggcgttcgat ccagcggatc attttacccg cgatgtcgat
      841 accggtggtt ttttctattc cttccagccc tggcgacgca ttcacttcca tcaccaacgg
      901 cccgcgatta gcacgcagaa tatccacacc agcaacgtcc agcgccatcg ttcgcgcggc
      961 ttttatcgcg atttcacgct cctgtggtgt gatactggcg acgcttgccg cgccgccacg
     1021 atgcaaattg gaacgaaaat cgccctcttt cgcccgccgt tcaatcgcag cgactacttc
     1081 atcgccaaca accagacagc ggatatcgca cccttgcgcc tctttgatat attcctgcac
     1141 cagaatatgc gcgttcagac cgcggaaagc gtcaatcacg ctttccgccg cctgacgcgt
     1201 ctccgccagc acgacgccaa ttccctgcgt gccttcaacc agcttgacca ccagcggcgc
     1261 accaccgacc atgtcgatta aatcgctggt atcatccggc gaatgcgcaa tgcccgtgac
     1321 aggcaggtcg atgccctgac gcgccagcag ttgcatggaa cgcaatttgt cacgcgcccg
     1381 ggcaatggcg accgactcat tgagcggata gctccccagc atctcgaact ggcgcagtgc
     1441 cgccgtccca taaaaggtaa tggcggtgcc aatacgcggg atcactgcgt caaaatgggg
     1501 taacttgcgg cctttgtagt gaatagaaga cgccgcagga tttatgttca tatagcaaga
     1561 aagcggatca agaatttcaa ccaggtgacc gcgctgtata gcggcttcac gcagccgctt
     1621 acacgaatag agcgttccat cccgggacaa tatggcaatt ttcaccctgc acctctctgt
     1681 cagacctggt caaaagcccg cgtatcatac atcgacgtgg cagttttagc gcgtcgccca
     1741 accctgtttg tgcaaataat ccagaataaa tgggcggctt tctttaatga ttgttcggcg
     1801 gatatgatcg ctccaggtat cccggcgatt attgctgcca cgggtgaggt aatattccgc
     1861 cagttgctcg tcatactgcg ccagtgcgtc tttatccagc ggttgatagc tgttttcatg
     1921 caccaaaatg gaggccggta aacgcggctt aagatccgga ttatccgcag gccagccaag
     1981 gcacagccca aacagcggca gaacatgctg cggtaatttc agcagttccg tcaccgcttc
     2041 aatattattg cgcaggccgc cgatatatac cccgcccaat cccagcgatt ccgctgcggt
     2101 taatgcattc tgcgccatca ttgccgtatc aacgacaccg agcaacagtt gttccgccag
     2161 gccgagctga gcatccggac agatctgtaa atggcggtta aagtcggcac agaacaccca
     2221 aaactctgcc gcttgcgcta cgtgtttttg cccgccggtc agcgtcacca gttcttcacg
     2281 taacgctttg tcggtaatgc ggataatgct actgcactgc aaaaaactgg aactggacgt
     2341 cgcacgggcg ctgttaataa tcgcctcacg ctgcgcttcg gaaatgggtt catcagtgaa
     2401 atggcgaatg gagcgatggc cacaaataag ttcaatggtt ggcgtcatta tctttttctc
     2461 tttctgaacg tgaatattgc ggtggatggt tcatcagctg tggggcaaga cgttttgcca
     2521 cctgaagaat aaccaccacc gcagcgggaa gcatgagcaa aacaccgaga aaaatcatca
     2581 gaatctgcac ttctggccga gaaaatggct caggtagcga cagggagtcg cttaccgaca
     2641 gcagcgccac cgccagcaac atcattccga taaattccag tatcaacacg cttttaggca
     2701 atttaccgat cgcgcgcata cgcttccctc tgcaaagtga gccttcagtc taaaactttt
     2761 cactgtattg tgtttaacag ttatagcttt tagcaattaa tgcaacaggt taaacctact
     2821 ttcagcgaat acattttagc gtgatcatta caggcataaa tctatgagga gagaaataat
     2881 gcaaaccgtt atttttggtc gtccgggttg cccttactgt gtgcgtgcaa aagatctggc
     2941 tgagaaattg agcaatgaac gcgatgattt ccagtatcag tatgtagata ttcgtgcgga
     3001 agggatcact aaagaagatc tacaacaaaa ggcaggtaaa cccgtagaaa ccgtgccgca
     3061 gatttttgtc gatcagcaac atatcggcgg ctataccgat tttgctgcat gggtgaaaga
     3121 gaatctggac gcctgatcgt ctgacaagcc ctcgcattga gggctttatt gattttttct
     3181 gtgctgtggt ttaaacaaac tactgataaa taagaaacac aatgccccca gcgcacacca
     3241 gaacaccgca cttaataacc atgccagctc ttgccagaat gagcgcgtcg gtgaaaaaaa
     3301 cagccgcata atgagcatcg agcagggtgc cgccagcatt gcgccaaaca gaggtttcag
     3361 gacttctcta cgatgtgaaa agaagctggc aactgcacca ggaagaatga aaaatagcaa
     3421 accgatttca ggatgcccgg cagcccgaaa agcgcctttc atgtgcgtcg ccagaaaaag
     3481 acacaccaca atgaagagga caaaacagca gattgccccc gcccaacgtt gtttatgttt
     3541 cactcgttcc tcctgacact gcgtctatcg aacacatttt tcgccagtgt ggcgttcagt
     3601 aagataaagc cgcttcgcat tccatgctaa tataggccaa cgcaattcat atagccgttg
     3661 atacctaatg tgattacact agtaaaatat attgttactt tactatcgtt taggtgcgct
     3721 gaatgaatct gcgccctgaa ttctggtaaa aacattatcg taaattacca tttctttcaa
     3781 cagcttacta gtaaacaaga agttagcctc cgtgaatata aacgtcgccg aattgttaaa
     3841 tgggaattac attctgttat tatttgtggt cctcgcgctt gggctatgtc tcgggaaatt
     3901 acgacttggt tcgatccaac tgggtaattc cattggcgtt ttagtcgtat cgctgttatt
     3961 aggccaacaa catttcagca ttaacaccga cgcgcttaat cttggcttta tgctgtttat
     4021 tttctgcgtc ggggtcgaag ccggaccgaa ctttttttcc attttttttc gcgatgggaa
     4081 aaattaccta atgttagcac tggtgatggt tggcagtgcg ctggtgatcg ccttagggtt
     4141 aggtaagctg tttggctggg atattggcct gacggccggt atgttagctg gctctatgac
     4201 gtcgacaccg gttctggtcg gtgctggcga tacactgcgt cattccggca tggaaagcag
     4261 gcagctctca ctggcactgg ataatctgag cctcgggtat gccttaacct atttaatcgg
     4321 tctggtgagt ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc aggacttaca
     4381 gaccagcgcc cagcaaatcg cccgcgaacg tggcctggac actgatgcca accgtaaggt
     4441 ttatttaccg gtgatccgcg cctaccgcgt cggcccggag ctggtggcct ggaccgacgg
     4501 caaaaatctg cgtgaactgg gtatttatcg acaaactggc tgctacattg aacgtattcg
     4561 acgtaacggg attctggcaa atccagacgg tgatgcggtg ctacaaatgg gcgatgaaat
     4621 agcgttggta ggctatcccg acgcccacgc ccgactcgat cccagcttcc gtaacggcaa
     4681 agaagttttc gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag tggtcgttaa
     4741 aaaccataac gccgttggca aacgtctcgc acaactgaag ttgaccgatc acggttgctt
     4801 ccttaaccgc gtcattcgta gccagattga gatgccgata gatgacaacg tcgtgcttaa
     4861 caaaggtgac gttttacaag tcagcggcga tgcccgtcgc gtaaaaacca tcgccgatcg
     4921 catcggcttt atctcgattc atagccaggt cactgacttg ctggcattct gcgccttctt
     4981 tgttattggg ctgatgatcg ggatgatcac cttccagttc agcacattca gtttcggcat
     5041 gggaaacgct gccgggttgt tattcgccgg aattatgctg ggatttatgc gcgctaacca
     5101 cccgaccttc ggttacattc cgcagggtgc attaagcatg gtgaaagagt tcggcttaat
     5161 ggtgtttatg gcaggcgttg gtctgagcgc cggtagcggt attaataacg gcctgggcgc
     5221 gattggcggt cagatgttga ttgccggatt gattgtcagt cttgtgccag tggttatctg
     5281 tttcttgttc ggtgcttatg tattgcgaat gaaccgcgca ctgttgttcg gcgcaatgat
     5341 gggcgcacgc acctgcgcgc cggcaatgga gatcatcagt gatacagctc gcagtaacat
     5401 cccggcgctg ggctatgcgg gcacctatgc aatcgccaac gtcctgctga cgctggcagg
     5461 gacaatcatc gtcatggtat ggccaggatt aggataaaac tgaagttgcc ctgaaaatga
     5521 aatttttttt gcacaaccgc agaacttttc cgcagggcat cagtcttaat tagtgccact
     5581 gcttttcttt gatgtcccca ttttgtggag cccatcaacc ccgccatttc ggttcaaggt
     5641 tgatgggttt tttgttgcct gaaatttatc tactacccag caatcctctc aaccatcctc
     5701 aaaatctcct cgcgtgatag cggcttacgg tcggtgacaa aatgcagcgt cattccctca
     5761 ataaacgcat caagcgcgcg ggcggttccg ggttcaaacc attgttcgag cgtttgttga
     5821 ctgcgctgca tccagttttg cattaccgtt tttaagagcg gttttcggct agccagcgcg
     5881 tagagctggt acatcagctc catattatcc ggcgttgcga cctgtgagct gtagatcata
     5941 tcggtgatag cctggcatgc gcctggagca tcactaacat cgctaaaaaa tgcctgatat
     6001 tgccgggaca tgatctcagt aaaacggctg aacgcctcca gcaacaactc atcaattcct
     6061 gaaaaatagt aggtcatcga tcccaacggc accccggcaa gggtagcaat tttgcggtgt
     6121 gtaacagcat gtattccgta aagtttcacc gcctccagcg tggcctggat aattttttct
     6181 cgtcgttgcg gatcgttagc gcgacgcatg tcatttcctc tctctgcaat tgtgtacaaa
     6241 tgtacacagt cttgctaatg ttgtcttccc tcttcttatt ttgacgtggt ctatgaccgt
     6301 aaattcttca cgtaatgcat tgaaacgccg aacctgggcg ctgtttatgt tcttcttttt
     6361 gccaggcctg ttaatggcgt cctgggcaac ccgtacgcct gctatccgcg atattctctc
     6421 tgtctcgatc gctgaaatgg gcggtgttct ctttggtctg tcgatcggtt cgatgagcgg
     6481 tattctctgc tcggcgtggt tagtgaaacg ctttgggacg cgtaatgtca tccttgtcac
     6541 gatgtcctgc gcattgatcg ggatgatggt attaagtctg gcactctggc tgacatcgcc
     6601 cttgctcttt gccgttggtc tcggcgtctt tggggctagt tttggttctg cggaagtggc
     6661 gataaacgtt gaaggtgccg ccgttgagag agaaatgaat aagacggttt taccgatgat
     6721 gcacggtttt tatagcctgg gcacgctggc aggcgctggt gtcgggatgg cactgacggc
     6781 ctttggcgtt ccggcaacga tgcacatttt attggcggcg ctggtaggca tcgcgcctat
     6841 ttatatcgcc attcaggcaa tccctgatgg tacgggcaaa aatgctgccg atggcaccca
     6901 gcatggcgaa aaaggcgttc ctttttatcg cgatatccag ttgctgttga ttggtgttgt
     6961 agtgctggcg atggcctttg ccgaaggttc tgccaacgac tggttaccct tattaatggt
     7021 tgatggtcac ggttttagcc ctacttccgg ctcgctgatt tatgccggtt ttactctggg
     7081 gatgaccgtt ggacgcttta ccggcggttg gttcattgac cgttacagtc gcgttgccgt
     7141 ggttcgggcc agtgcactaa tgggggcgtt gggtattggg ctgattattt ttgtcgatag
     7201 cgcctgggtc gctggggtgt ctgttgtact ttggggactg ggtgcctcgc tgggcttccc
     7261 gctgaccatt tctgccgcca gcgataccgg ccccgatgca ccaacccgcg tcagcgtggt
     7321 agctacgacc ggttatctgg ctttcctcgt cgggccgccg ctgctgggct atctcggcga
     7381 acattatgga ttacgtagtg caatgatggt tgtactggcg ctggttattc tcgcggctat
     7441 tgtcgcgaaa gccgtcgcca aacccgatac caaaacgcag acggcgatgg agaatagttg
     7501 atgagcatta aattaattgc ggtagacatg gatggtactt tcttaagcga tcaaaaaacc
     7561 tataaccgtg agcggtttat ggctcagtat cagcaaatga aagcacaagg cattcgcttt
     7621 gtggtcgcca gcgggaatca atattatcag ttgatctctt tctttcctga aattgctaat
     7681 gaaattgcct ttgtggctga aaacggcggc tgggtagtga gcgaaggcaa agatgttttt
     7741 aatggcgagc tgtcgaagga tgcgtttact actgtcgtgg aacatttgct gacgcgcccg
     7801 gaagtggaaa ttattgcctg cggaaaaaat agcgcctata ccctcaaaaa atatgacgat
     7861 gccatgaaaa cggtggcgga aatgtattat caccgtctgg aatacgtcga taactttgac
     7921 aacttagaag atatcttctt taaatttggc cttaatctct ctgacgaact gatcccacaa
     7981 gtgcaaaaag cattacatga ggccatcggc gatatcatgg taccggtcca taccggcaac
     8041 ggcagcatcg atctgattat ccccggtgta cataaagcca atggccttcg ccaactgcag
     8101 aaattatggg gaatagacga cagcgaagtg gtggtctttg gcgatggcgg taacgatatt
     8161 gagatgctgc gtcaggcagg gtttagcttt gcaatggaaa atgccggcag cgcggtcgtc
     8221 gcagcggcaa aataccgggc aggctccaat aaccgtgaag gcgtactgga tgtgatcgat
     8281 aaagtgctta aacacgaagc gccatttaag caataaaaaa ggcagcacgc ccggcaacac
     8341 aatacctgaa tgaaggctgc ataattcgcg gaaattaacc cgctgcatta tttcaggcga
     8401 aatgattatg aaaaattgtc tactgttggg cgcgctttta atgggcttta ctggtgtggc
     8461 gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac aaagtggttg tggttcctga
     8521 ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg ccgcaaaccg tctatgtggc
     8581 tcctgcgcca gctccggctt accgcccgca tccttatgtt cgccatcttg ccagcgtagg
     8641 tgaagggatg gttatcgaac accagattga cgaccatcac cattaattcg acaatgcctg
     8701 atcgcacaaa gcaatcaggc atttttttac ccttcgtgcg aatttcccat ctgtttatct
     8761 tttaaaaaga taaccatcag cgacagccac aaaatgccgt tgacaaggtt gaagagatta
     8821 aacagtccgt tgcccccgtt cagccaggca tgtttgctga tttcaatacc gacggtaaag
     8881 atcagcattt gcagcatccc catcgccgca gaaaccgtac ctttactcat atcgctggca
     8941 aacagggtta atcgcaccag tcccgcattc gccagaccaa taccgaaagc ataaatactt
     9001 aacccggcgg tcatccataa atacgcgtgc gatgagataa ccgttgccgc agcagcgacc
     9061 aacagaccaa tcataatcgg ccagccgccc ataataatca gcgaacgtac ggtgcggcgc
     9121 gaggtcagac gcgctaacag caagttaccc gcaattaacg ccccaaaaat aggcacttgc
     9181 agcaggccat attcatagct gctcaactgc tcaccggtaa taatgataat cggcgactgg
     9241 gcgatccacg ccagcaacgg caggctaacg aatcccagcg ccagcgcccc cgccacaaag
     9301 cgaccgttct tcagcaccag cttatagtca cgaccgagtt ctttcagcga cagtttctcg
     9361 cctatacgcg tggcggtttc aggcatagct cgttgcagac cgaaaaagga gatcgctgcc
     9421 aatgcggcaa acaagacaaa catcccttcc cagggcagca catggatcca cgccgcgccc
     9481 accagcggac caagtagtgg agcaatcagc gccacgttcg ccattagcgc ggtgatcttg
     9541 atacaaaccg cctcttcgaa ggattcctga attgcggcgt atcccacagc gccaatgaaa
     9601 cagaggctta taccctgcaa gaagcgcaac agggtgaatt gttcaatgtt ttgtgccagt
     9661 aatattgcca gacaggtgac gataaaccac accactcccg ccagcatcac cggacggcgg
     9721 ccaatacgat ccgacaacgg ccccaggagc cattgtaaaa acatcccgcc cgccagatac
     9781 gcagtcatcg aagtaggaac ccaatcaatg cccgcctgat attgttccac cacggccaac
     9841 ataccgggtt gaatcatatc gttgccgata taggttgaaa attcgtaaag caccagacag
     9901 agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg aagctaattt attttgcatg
     9961 caatttcttc gccaataata atcgcgcaaa gtttaataaa agcgcagcta acgagaaagc
    10021 gaattttgta gctgaaacca cggttaagca cattcttaca ttattgcgag tatagctacg
    10081 ctttctttaa gttttattta acctttgccc gttacaatca cctaccgtaa acaggccgct
    10141 tgagggaaat aagacgatgc cgctttaccc agtttaacct gcactttatt ctcaacgact
    10201 tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa gttatgctgg aaaatttgaa
    10261 tctctctcta ttctctctta ttaacgcaac gccagactct gcgccgtgga tgatctcgtt
    10321 ggcgattttt attgctaaag atttgattac cgtggtgccg ttgctggccg cagtactttg
    10381 gttgtggggg cttacagcac aacggcaact ggtgataaaa atcgctatcg cgctggcggt
    10441 cagcctgttt gtgtcctgga cgatgggaca tctttttccg cacgaccgac cctttgtcga
    10501 aaatatcggc tataacttcc tgcatcatgc ggcggatgac tcattcccga gcgatcacgg
    10561 tacggtgatt ttcacctttg cactggcatt tttatgctgg catcgcctgt ggtccggctc
    10621 acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc gtttatcttg gcgtccactg
    10681 gccgctggat atgctcggtg gattgctggc aggtatgatt ggctgcctta gcgcccagat
    10741 tatctggcaa gcgatggggc ataaactcta tcaacgtctg caatcgtggt atcgcttctg
    10801 ttttgcatta ccgatccgca aaggctgggt gcgtgactga tctctgctaa aaagtgtagt
    10861 attgagcggc tcgcttcaat aactattcag agggattatg gaaacacgtc gcgaagagcg
    10921 tatcgggcag ctgctgcaag aattaaaacg cagcgataag ttacatctta aagacgccgc
    10981 cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat ctgaacaacc acagtgcgcc
    11041 cgtcgttttg ctcggcggct atattgttct ggaaccacgc agcgccagcc attacctgtt
    11101 aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg gcggcaaaac tggctgcgac
    11161 gctggtagaa cccgatcaga ccctcttttt tgactgtggc accaccacgc cgtggattat
    11221 tgaagcaatt gataatgaaa tcccttttac cgccgtttgt tattcgctaa atacttttct
    11281 ggcgctgaaa gagaaacccc attgccgcgc gtttctttgc ggtggtgaat ttcacgccag
    11341 caacgccatt ttcaaaccca tcgattttca gcaaacactg aataattttt gcccggatat
    11401 cgctttttat tccgctgcgg gcgtgcatat cagtaaaggc gctacctgtt ttaatcttga
    11461 agagttgccg gtaaaacact gggccatgtc gatggcgcaa aagcatgtgc tggttgtcga
    11521 ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt gacctgaaac gctttgatat
    11581 tgttgtgagc gattgttgcc cggaagatga gtatgtgaag tacgcgcaga cgcagcgtat
    11641 taagttgatg tattaatgac gtataaccgg atgacgtttc gcgccatccg gttatcagaa
    11701 gattaagaga accagctgcc gaaccactga tggaatttca tcatcacgaa atcccacatc
    11761 cgaccaaaga atccgccctc ttccacattt tccatcacga tcagcggacg ctgctcaatg
    11821 gatttaccgt taagctggaa atcaatggtg ccgacaacct gacctttttt cagcggtgcg
    11881 gtaagctgcg gttccgttaa cgtataactc gccttcaggt ttttcagctg cccacgcggt
    11941 atggtcactg agcccgcttc gcctgccccg agattcactt cgctcttatc accaaaccag
    12001 acgcgctgag tcacaaatgt ggcatcaggt ttaattggcg tcacggtttc aaagaagcgg
    12061 aaaccccagg tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc
    12121 agcactacgg agattaaacg catatcgccc tgggtagccg aagcaaccag attatatccc
    12181 gcgcctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga
    12241 cgattacggt taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg
    12301 tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta
    12361 ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt ggtcagaccc
    12421 agttttttgg cataaccatt catcaaacca ataaatgact cctggctccc ggcaacgtaa
    12481 tcagccagcg caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct
    12541 gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga
    12601 tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt aattttatcg
    12661 gccttaagcg cctgcccaac cacatagctg gtcataattt tagtcaggct cgcgggatcc
    12721 agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc
    12781 catgcacggg catccacgct cggcgcttca acggtttgtt ccgccgcgaa tgccgttggg
    12841 gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc
    12901 gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc gtatgataag
    12961 ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa
    13021 agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc
    13081 ttaaaagtta actttacttc accatcggag tcctgtttag cctgtggctg cctgtcggaa
    13141 aaggggtgag tatgattacg ctgtggggtc ggaataattc aaccaacgta aaaaaagtat
    13201 tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg
    13261 ggataaatca cgatactgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc
    13321 gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg
    13381 cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcagaaa
    13441 aatggatgga ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat
    13501 tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt
    13561 gcgacgccct gtttgccctg ctcgatgcgg aattggcaaa agtaaaatgg ttctcaggcg
    13621 acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg
    13681 gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc
    13741 ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta
    13801 attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat
    13861 cacgaattcg ttgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt
    13921 tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat ttttgctgcc
    13981 actgggggaa tttgtcgctg ttatagaaag ccatgccgct cacggcgggc gaatctttcc
    14041 agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa
    14101 agcccgaata gttgattccc caggttgcca gcggccagcc gtagtttttg cctttttgcg
    14161 gaatattaat ttcatcacca ccgcgcgggc catgttcatt cagccatagt gcattactcc
    14221 acggattcat cgccattcct tgcggattac gaatgccata agaccagatc tcggcgcgca
    14281 caccggattc ctttataaaa ggattatcat ccgggatttc tcccaggtcg gtcagacgca
    14341 ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg ttttcgccca
    14401 gagcaataaa aagataacct ttaccgtcaa ataccatccg cccgccaaaa tggttgccgg
    14461 tagacagttt tggcatctga cgaaagacgg tgcggaagtc ggtcactttt gagagatcat
    14521 cacttaagcg accataaccc acagcagttc cggctttgcc atcatcgcca acttcggaat
    14581 aacttaacca gatgcggcgc gactgagcaa aatcgggcgc taaaaccacg tccagcaggc
    14641 cgccctgccc gtgcgcccaa acgtccggca ctccggaaag cggcgcagat aatccttttc
    14701 ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg
    14761 gtaaaaaggc cagcgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg
    14821 ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt
    14881 gtcgatgcat aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagagaa
    14941 caaattcatt tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt
    15001 tcaggccttc tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat
    15061 cagacaattt tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc
    15121 actcatcaaa catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca
    15181 ctcttcctga tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag
    15241 tttaaaaaag ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt
    15301 cctcagttgc acgtatgcgg cgaggtcaac gcgtgcgtcg atcaaccggt tcagcaggtc
    15361 gattcgctgt ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta
    15421 aatagtatgg ctgttttttg agcaaaatgc tgtccaggat caattcttca cgccgaatta
    15481 acaaaattta catttccttc aactccgcca gcgaatgaga ttgcgataca aattttatcc
    15541 agaactctgt agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga
    15601 gcaaagtaac tccccagccg aaaatcggct ttgtttccct tggctgcccg aaaaaccttg
    15661 tcgattcaga gcgtattctc accgaactac gcactgaagg ttatgacgtg gtaccgagct
    15721 atgacgatgc ggacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag
    15781 aatcactgga agccattggt gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt
    15841 gtctgggggc aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg
    15901 ggcctcatag ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac
    15961 acaacccgtt cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg
    16021 cttatctgaa aatttctgaa ggctgtaatc accgctgcac cttctgcatt attccgtcta
    16081 tgcgcggcga tctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg
    16141 ttgatgcggg cgttaaagaa attctggtga tctcgcagga tacttccgcg tatggcgtcg
    16201 atgttaaaca tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc
    16261 tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc
    16321 cgcatgtgga cgacgtcatc ccactgatgg cagagggcaa aatcctgccg tatctggaca
    16381 ttccgttgca gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag
    16441 atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct
    16501 caacctttat tgttggcttc cctggcgaga cggaagaaga tttccagatg ctactcgact
    16561 tcctgaaaga agcacgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg
    16621 cagacgccaa tgccctgcct gaccaggttc cggaagaagt gaaagaagaa cgctggaacc
    16681 gtttcatgca gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggtcgtg
    16741 aaattctggt gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag
    16801 atgcaccgga aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg
    16861 atatcctgcg agtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt
    16921 aattaattca ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct
    16981 aatctgcgcc ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa
    17041 tcgttcgacg gtagtacatt catttgaatg cgctacaccg atactggtgc ttaaattaat
    17101 gcttcccacc tggaaatgaa cgatttcttt tagaatcgat agcaactctt ccagcgtgcc
    17161 gccagtataa atcaccgcaa attcgtcacc tccgaagcgg tagactttac ctttatctac
    17221 cgggatctgt gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg
    17281 accgaaggtg tcgttaatgt ttttgaattt atcgacgtca aacaggcaca aataaccgcc
    17341 tttttccggc agcgctttta aatcttcgtc aaaggatttt ctgcccatcg ctttggtcag
    17401 gtcgtccagg aatgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa
    17461 gaaaagcccg ccatccagta cgcgttcaaa atggttatta ataatggctt ctttctccac
    17521 aataccacga ttaatcactc tgcgtaccag atagaggcaa ctggcacccg atatcactgc
    17581 caccaatgcg gcaaagaaaa taatcaatgt acatacctga aaataaacgt cttttttata
    17641 agcaaatacg tacaatccgg tcggcaaata ggagaagtgc tccattgctt ccgaaacggt
    17701 gaagatggcg tttttagcgt tatattcatc accggtcacc gatttaccgt tcttgaacag
    17761 ggagaacatc aggttctcgc tatccatcgc cagcgatttt gacagtaaag aaaaacgttc
    17821 cagaccaagc aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa
    17881 ggttttacca tcaaaagaga taaaccctgt atttttgaca tgtcgatact taacaacctg
    17941 cataatatct gcacaataat ttttctcaaa ctcctttacc tgaatacatt cgtcactgga
    18001 aatcagataa ggaccgacat cgtacaaata agagggtgaa accgagtcgg cgtaagctgt
    18061 gcgtaagcca accaacagct gttcctccgt aggatttagc gtgttgatca gatcaattgc
    18121 cacactccgc cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg
    18181 tttcaggcca tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga
    18241 aatcatgata aaaatcagca gactgactgt aagtacgaat ttattgattc tggacatacg
    18301 taaactactc ttttactaat tttccacttt tatcccaggc ggagaatggc atgggtttct
    18361 ggtacagata cccctgagcc tgaacacatc ctaatttgat taattcacgc gcaacgtctt
    18421 cgctctctac gccttccgca atcaccggga tccggttata gcgtgccaga tcgaaaataa
    18481 tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt aaattgcgaa tccaccttta
    18541 tactgtctgg attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat
    18601 caatcgatac ccgtacccct tgtttacgca gttgttcaat attgagatag aaatgttcat
    18661 taattcgctc gatcacggtt tcggtgatct caacacaaag gctgttcgcc ttcaggccat
    18721 tttctttcat gatggtagta aaacgctcat gaaagtgtgg ttcgtttaac tgccagggcg
    18781 aaacattaat atgcagtaag aaatcatcct tcagcgacgc acgctgacta aaatgtctga
    18841 attcacccat cgcggtttta ataatttgat agcccagctc attgataaac ccgatatctt
    18901 cagcaatggt aatgaacttc agcggtgaaa taatcccccg ctccgcagac acccaacggc
    18961 acaacgcctc cgcgcccaaa atggtgcggg tgttaatatc aacaatcggt tgatagacca
    19021 ggaatagttc gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt
    19081 ctatttcgtt gagtttcgca tcatcacaga aaataaactc tgagaacata tggttgcgga
    19141 tactggaaat cgccagtttc agcttttctg cgtaactcca ggcgttttcc ccctgcaaag
    19201 gttcacaaat agcagactta tggatgcgca gattgcggta gccaaaactg ctgtcaaaat
    19261 cgtttaccgt cgctatctta cgggtaaacg tctgtacatt ttcccggcac aggagtaaat
    19321 agagatcggc attaaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct
    19381 gacacaattt cctgacgatt ttattgcgtt catcaacacc aaacaccgct tccacagctt
    19441 ccagaccaca cagtttgatg atccccacag ccagatactg attattgttg gactcaataa
    19501 tcgccatatt tttaataaaa ccgctttcct gatcgtaaga gatcagcgag agcaatttat
    19561 ttgcccgttc aatttcagca tctttcattt ccgcgatcga atttttgatg ctgataatct
    19621 ctttcgcatc gccttcacga atcttcaggg cctcgatttg atccgtagag tcaggcaaaa
    19681 acgccagcgt attgaccaaa tctgtcattt cagaaaattg acgacggaaa actcgagtgg
    19741 tcattaaata aaccaccagc aatacgataa taatgaaagc aaacggcaga taaaaaagtt
    19801 tatctgatgc gttcaataaa ttaccaccag gataataaaa atagatttcg aataatgcgt
    19861 tgtttggcac cgctgatgtt ttttcaatat attccgtatc gacagaatca ataatattat
    19921 aattaccttc gctattataa ataatcaatt tttcacgagc gagttgattc ttagcaatga
    19981 tctcatgacc ttgctttaaa aatagaactt catcattagc gctgatgtga tcaagatact
    20041 cgttcagata tcgccagttt aaatcgacat gaattttatc taccgctaac aaatcatgat
    20101 ctttgccgtt gatgtgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat
    20161 tgtatgccgg gttccagctg ttgttagagt catctgaagc catcgaccac ggtcgtttcg
    20221 atgggttaaa atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt
    20281 gttcctgatg ataaacaacg attttatcat tcgccctccg cgaaaagaga taatatttat
    20341 tatcatcaag taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca
    20401 cggcacgttg aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg
    20461 ccgaacgttc aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc
    20521 tatcggcacg ttttaccgcg cgttccgata ccagttgata gttaatgatg aaaaaaagac
    20581 caataaatga caatgctgcc aataaaaata aaattatcag ccttatcttt atcttttcgt
    20641 ataaactcag catgttaata catttaaaaa aataactgtc tgaacgctgt gccgctgcaa
    20701 aatagtccct tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag
    20761 ttacaacact tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt
    20821 caaacgtaac tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa
    20881 attgaacgcc agtacggtca gaaaaatagc cagggccgga aatacagcga catgcggcgc
    20941 gataaccata tccgctcgtg cctcattgag cattgcaccc cactctggtg tcggcggctg
    21001 cgcaccgagg ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat
    21061 ggtgaaaaac accacgatag aagagacggt cccaggcaag atatgacgca acaaaatggt
    21121 catatcgctg gcaccaatac tgcgtgctga ctcgataaag gtttgctgtt tcaacaccag
    21181 cgtgttgccg cgcaccagcc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat
    21241 cacgttagca atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg
    21301 gaaggcaaac agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata
    21361 tccagccagc aagcccagca acgtcccgat cgccgcaccg ataaacacgg caaacacgcc
    21421 cgccgccagc gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga
    21481 atcgacacca aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata
    21541 attttcggcg tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat
    21601 aacgaacaat gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg
    21661 ccacggtgta cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg
    21721 tcgccagtta aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa
    21781 agcacatcca ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc
    21841 tgaatcactg ggtaatcgcg catttctacg gagtcaacga gtaagcgtcc aagtccgggc
    21901 cagttgaaaa ctttctccac cacgatggaa ccaccgagca agaagccaaa ctgtaagccc
    21961 atcatggtca ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt
    22021 tcgctcaccc ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaac
    22081 gacgcgcggg taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt
    22141 aaaatgtagt gctgccaact gtctgctccc acggtaggca gccagcccag ttcaacggag
    22201 aatacctgaa ttaaaagtat ccccagagca aatgccggaa acgagatccc cgacaccgca
    22261 atggtcatac tcaatcgatc cggccaacgg ttacgccaga cggcggcgat aattcccgcc
    22321 gccataccaa atatcaccgc ccagaccata ctggttatgg tcagccagag cgttggcata
    22381 aagcggctgg caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc
    22441 ccctgcacag cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc
    22501 aactgctgac gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc
    22561 gccggatcgc cgggcagcat atggacaaat aaaaacacca gcaccgagac gataaacagc
    22621 gtcggaatca accccagtaa gcgtttgata acgtaattaa gcattccact ccctgcgttg
    22681 cttattgcaa atccgcgtct tcaaagctga agccggtgtc tggcatgatc caaaaaccgg
    22741 tcaggttttt actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgact
    22801 cttgccagat gatatcctgc gccgccttat acaagcgggt cttttccgcc ggatcattag
    22861 ttttcagtgc ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa
    22921 acagcgttgg tggccagttc tgcgaggcaa acagcggcga tagagcccag tcggcttcgc
    22981 cggttgaagc cgaccagcca gtgtagaaca tccgcacgcc gctctctttt tgccctttac
    23041 cttcaacttc tgccgcccgc tgtccggcat ccatcgcagt cacctgggct ttaatcccaa
    23101 cctgcgctaa ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg
    23161 acgaccacag cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg
    23221 ctttcactgg atcgtaaggc cacggtttat aactttgcgc gtaggcgata ctcggcggta
    23281 ccacaccagt agctggcgtt gcatagcccg cgaaggccac tttcaccagc gccgggcggt
    23341 taatggcgta attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt
    23401 tcatactgat ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt
    23461 tctccagcag tgcggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt
    23521 gcagcattgc cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt
    23581 tgggcaatcc tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat
    23641 tccaggtatc cagttcatac ggcccggttc ccaccggatg aaaaccaatc tccttgccat
    23701 atttttccag cgctgccggt gatatcatcg cggtcgccgg atgggcaaga atattaataa
    23761 acgctgagaa tggctgtttg agggtaatct ttaccgttgt cggatcgatc gcttccgttt
    23821 tggcgatatt cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt
    23881 ccagattcgc tttcaccgcc gctgcgttga aatcggtgcc atcctggaat ttaatgcctt
    23941 cccgcaattt cacggtgtaa gtaaggccgt catcggaaac ggtgtaactc tccgccagca
    24001 cgtttttcag tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta
    24061 cggcctgaga taacgtgtca tttgcgtcat acggatcgag cgtggtgaag ttcgatccca
    24121 ccgccaccac cacctctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc
    24181 ccagcgccac taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt
    24241 tatctacgca tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg
    24301 cattgcaacg agacggctgc aacctcttcg ccacgcagat gaatattgct gggaagatcg
    24361 tccgacagca gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc
    24421 agtaatttac gcgtataagg atgctgcgga ttttcaaaga ccgcgcgccg tggaccaatt
    24481 tcaacaattt gcccgagata catcaccgcc acacgatgac taatccgctc aaccacggcc
    24541 atatcgtggg agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag
    24601 ttgataatct gcccgcgaat agaaacatcc agcgccgaga cggcttcgtc ggcaatgatc
    24661 acttttggat tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa
    24721 aactcatgtg ggtaacgcca ggcatgttcc ggtaacaggc ccacgcgctc cagcaaccac
    24781 gcaacgcgtg cagccgcttc tttacctggc aataaaccgt gtacgcgcag cggttcgaga
    24841 atcgaatcac cgatagtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac
    24901 tgaatatcgc ggcgcaatgc ctgaagttta ccgggtgaca aggtatcgat tcgctgaccg
    24961 ttaaagataa tttcgccgcc ctgcgattcg accaggcgca acaacgcccg cccggtagtg
    25021 gatttaccgc tgccagactc gcccaccagc gatagcgttt cgccaggcca gagatcaaaa
    25081 ctgactttct caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa
    25141 gggaaacggg tgaccagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc
    25201 tgctcgatgg atgactcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg
    25261 ggataatcta accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggta
    25321 taaggatgtt gcggtgcatg aaaaatctgt tcgacgctac ccgtttccac cgcctcgccc
    25381 tgatacatca ccagtacccg atcggcaatc tctgccacca cacccatatc gtgagtgata
    25441 aagataacgc ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc
    25501 tgaatagtga catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat
    25561 gacagcgcca tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcggataa
    25621 cgtgaaagaa tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg
    25681 accatcgctt cttcacgact ggcgttctga tgcagacgaa tagattcagc aatctgttca
    25741 cccacagtaa ataccgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc
    25801 gcaccgcgca catggcgcat ttgtgcagcg ctctgctcgc taagttcaat tacttcgcga
    25861 ctgcgccgct gcaacagcat tttatcgcac tgcactaaac cgcccgcctg ttccaacagg
    25921 cgcatcaatg ccagcgcagt cactgactta ccggagccgg attcgccaac aattgccagc
    25981 gtctcaccac gttgcaggct aaaagagaga ttgcggaccg cagctatttt ctgctgctcc
    26041 tgcataaagg caatattcag attttcaacc gccagcacat caccggcatc aagttcatca
    26101 ctgtgtggca acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc
    26161 gtagccccag gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg
    26221 gtcgatagcg attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg
    26281 ctcgcaggct tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tatcatatgc
    26341 cgccagcgcg cggatgaaga cttcgcccgt accggtacag gaaaccgcca cactggcgtt
    26401 attggcatag catccggccc ccactaaggg actatcgcca actcgtccgg gtaatttatt
    26461 ggtcattccg cccgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc
    26521 ccctacggtg cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt
    26581 tgccccttcc tcgcgcgctg ccattagttg ttcataacgc aaaggtgtgg agaaaatctc
    26641 cggcgagacg cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat
    26701 catcacatgc gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg
    26761 actaacgccc gccaccgcac cggctttcag ggtgttacca tccatcagac aggcgtccag
    26821 ttcatgggtt tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc
    26881 ttccagcaga cgcaccgctt ccgtcaccac atccagcgca ctttcgcccg ctaccagcat
    26941 tttctgcccg gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag
    27001 actcatctgc gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc
    27061 catagtattc gtccattaag agtggctcgc cacttttgct ataaatatca gaatttttat
    27121 gaatgactca ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc
    27181 agcgctacgc ccgaggtggc atacagtctg tggcgttttt ctgacataat aggcaaattc
    27241 gattttgcct ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca
    27301 ccgcgcttaa tgagatgctt tctcgcgtta ccccactgac cgcccaggaa acgctgccac
    27361 tggttcagtg ttttggccgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg
    27421 ggtttgataa ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc
    27481 aaccgctgcc cgttgctggt aaatcctttg ccggtcagcc gtaccatggt gaatggcctg
    27541 cgggtacctg cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg
    27601 tgatgcagga gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagcgcgta
    27661 gcgggcaaaa tattcggcgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg
    27721 cggggactcg cctgactacc gctgaactgc cagtgattgc ttcgctaggg attgccgaag
    27781 ttccggtgat tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc
    27841 ccggtcagcc gctgggcgac ggccaaatct acgataccaa ccgtctcgcc gtacacctga
    27901 tgttagaaca gttgggatgc gaggtaatca acttagggat tatccgcgac gatccccatg
    27961 ccctgcgcgc cgcatttatt gaagccgaca gccaggcgga tgtggtgatc agttccggcg
    28021 gtgtttcagt gggtgaagcg gattacacca aaacgattct tgaagagctg ggggagatcg
    28081 ccttctggaa actggcgatt aaaccgggta aaccgttcgc gttcggtaaa ctttctaata
    28141 gttggttctg cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg
    28201 tacagccttt gctggcaaaa ctaagcggca ataccgccag cggcctgccc gcgcgccagc
    28261 gtgtacgcac ggcatcgcct ctgaagaaat cgccaggacg ccttgatttc cagcgcggcg
    28321 tgctgcaacg caacgccgat ggcgaactgg aagtgacgac caccggacat cagggttcac
    28381 atatatttag ctcctttagc ctcggcaact gctttatcgt gctggaacgc gatcgcggca
    28441 atgtggaagt gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt
    28501 aatggcggaa ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg
    28561 ttttgatttt gacggtcagg aagcattgaa agattctcgc gtgctggtcg tcggccttgg
    28621 tggactcggc tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct
    28681 gctcgacttc gacacggttt cgctctccaa tctgcaacgc cagacgctgc acagtgatgc
    28741 cacggtcggg caaccaaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca
    28801 tatcgcgatt acgccagtca atgcgctgct tgatgacgct gaacttgctg cgatgattgc
    28861 aaaacacgat ctggtgctcg actgtacgga taacgttgca gtacgtaatc aactgaacgc
    28921 aggctgtttt gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca
    28981 aatcacggtc ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt
    29041 tggcgagaac gcgttaacct gcgtagaagc aggcgtgatg gcaccgttaa tcggcgtaat
    29101 tggttcgttg caggcaatgg aagcgatcaa actgctggca ggttatggca aaccagccag
    29161 cgggaaaatc gtgatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg
    29221 caatccgggg tgtgaggtgt gcgggcagta actgccacct ccaggccaga taagacgcgg
    29281 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
    29341 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcatca accgccggat
    29401 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacac cctgccagta
    29461 agcagtccag cgcctgacgc ggggttttga aactcgcggc taacactttc gcctgcggcg
    29521 catgcatttt caataactgg tgtaagtcgg tcacagtctg aataccgcta ccgccctgag
    29581 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagtagcc
    29641 cttgtgctgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
    29701 taatagctgc cagcccctct gcggtcaccg gaactttcac cacgatatcc gcaataatag
    29761 aacgcaattt acgtgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
    29821 acagacgccc ctgaccgccc atcgcctcgt gaagttgcgg aagcacaacc tccagcggtt
    29881 ttttacccac tgcgataatg cttgggttag tggttacacc cgccagcgga aaaatacgtg
    29941 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
    30001 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
    30061 catcaatcca actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
    30121 tgattttcaa tattcaacgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    30181 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca
    30241 cgcaggatct gctgtatgac ccacgactct gtctggaagg ctgtgagctg tgcgctaaga
    30301 ccgcaccgga agtgattgag cgcgcgttga atggtttgct tattcatcgg gaaaagttaa
    30361 ccccggagca ttttacggcg ctaaccgact gctgcccgac acaggcgtta accgtgtgtg
    30421 gtgaagtgaa aagcgttgag gagatcatgg caaccgtttt gcgcgataaa ccgttttacg
    30481 atcgcagcgg cggcggctta acactttctg gcggagaacc ctttatgcag ccggaaatgg
    30541 cgacagcgct actgcaagcc agccacgaag ccggtattca tactgcggta gaaacctgtc
    30601 tgcatgtgcc gtggaaatat atcgcccctt ctctgtccta tattgatctg tttcttgccg
    30661 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    30721 tgctggataa tctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccac
    30781 tgattcaggg ctttaatgcc gacgaaacct ctgtgaaagc cattaccgat tttgccgccg
    30841 acgagctgca cgttagcgaa attcatttcc tgccctacca cacgctgggc atcaacaaat
    30901 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc
    30961 tcgactttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    31021 aaccatgacc acactgaaac tggacacgct cagcgatcgc attaaagcgc acaaaaatgc
    31081 gctggtgcat attgtgaaac cgccggtctg taccgagcgc gcgcagcact ataccgagat
    31141 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    31201 cctggcgaat cgcaccatct ggatcaaaca tgatgaattg atcattggca accaggcaag
    31261 cgaagttcgc gccgcgccga tcttcccgga atataccgtc tcctggatcg aaaaagagat
    31321 tgatgatctg gcagatcgtc ccggtgctgg ttttgcggtg agtgaagaga acaaacgcgt
    31381 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagaccgta caggatcgct gctacggcat
    31441 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg agggcaatat
    31501 gacctccggc gatgcgcacc tggcggtcaa tttcccgcta ctgctggaaa aaggtcttga
    31561 tggtctgcgt gagaaagtgg cggaacgtcg ctcgcgcatc aatctgacgg ttctggaaga
    31621 tctgcacggc gagcaattcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    31681 cattgaacgt ttcgctgccc tggcgcgtga aatggccgcg accgaaaccc gcgaaagccg
    31741 tcgcgatgaa ctgctgacga tagcagaaaa ctgcgatctt atcgcccacc agccgccgca
    31801 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc
    31861 taacggtcac tcggtatcgt ttggtcgtat ggaccagtat ctctatccat actatcgccg
    31921 tgacgttgaa ctgaaccaga cactggatcg cgaacacgcc atcgagatgc tgcatagctg
    31981 ctggttgaaa ttgctggaag tgaacaaaat ccgctccggc tcacactcaa aagcctctgc
    32041 gggaagtccg ctgtatcaga acgtcaccat tggcgggcaa aatctggttg atggtcaacc
    32101 gatggacgcg gtaaatccac tctcttatgc gatcctcgaa tcttgcggtc gcctgcgttc
    32161 gactcagcct aacctcagcg tgcgttacca tgcgggtatg agtaacgatt tcctcgacgc
    32221 ctgtgttcag gtgatccgct gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt
    32281 catcccggaa tttattaaac tcggtattga accgcaggac gcttacgact acgcagcaat
    32341 tggttgtatc gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    32401 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc ggtcgtgatg ccaccagcgg
    32461 caaagtgttc ctgccacaag aaaaagcgtt gtcagcgggt aacttcaaca acttcgatga
    32521 agtaatggac gcatgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga
    32581 atatgtcgtc gacaccatgc tggaagagaa cgtacacgat attctctgct cggcgctggt
    32641 cgatgactgt attgagcgag cgaaaagtat taagcaaggc ggcgcgaaat atgactgggt
    32701 ttctggcctg caggtcggca ttgctaacct cggcaacagc ctggcggcag tgaagaaact
    32761 ggtatttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    32821 cgacggtctg actcacgagc agctgcgtca gcggctgatt aacggtgcgc ctaagtacgg
    32881 caacgacgat gatactgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    32941 actaaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    33001 gggtacgtcg tctatctccg ctaacgtacc ctttggcgcg cagactatgg caacaccgga
    33061 cgggcgtaaa gcgcataccc cgctggcaga aggcgctagt ccggcctccg gtactgacca
    33121 tctcggccca acggcggtca ttggctcagt aggtaaactg cctacggccg cgattctcgg
    33181 cggcgtgctg ctcaaccaga aactgaatcc agcaacgctg gagaacgaat ctgacaagca
    33241 gaaactgatg atcctgctgc gcaccttctt tgaagtgcat aaaggctggc atattcagta
    33301 caacatcgtt tcccgcgaga cgctgctgga ggcgaaaaaa catccggatc agtatcgcga
    33361 tctggtagtg cgcgtcgcgg gctattccgc cttcttcacc gcgctctctc cagacgctca
    33421 ggacgatatc atcgcccgta ctgaacatat gctgtaatct tctcagcccg gcggcggcgt
    33481 cgccgggcta aatcattcac atcatcaatt tcagcctcgc tttcattcga aatataattt
    33541 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    33601 ctatgagcgt aaaagttatc gtcacagaca tggacggtac ttttcttaac gacgccaaaa
    33661 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattgagt
    33721 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    33781 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcagttgt
    33841 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    33901 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    33961 aagcattcgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    34021 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt
    34081 tagtgatcga caaactgcac ataacgctgg atggcattat gaaacccgtc accagtggtt
    34141 ttggctttat cgacttgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    34201 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    34261 cggagatgct gaaaatggcg cgttattcct ttgcgatgag caatgctgcg gaaaacatta
    34321 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    34381 aggcggtgct ggaaaataaa ccgccgttta atctttgatt atcaccaatt cagtttattg
    34441 agaaagtgca tgtcattata ccggatacgg cgtgaccgcc ttatttggca tacaaaatca
    34501 tgaaaattca atatattgcg atacactacc aggcctgata agcgtagcgc accaggcaat
    34561 cttgcgtttg tcatcagttt cagtagccct gaccatatca gggctactga taataactta
    34621 ctcgatccct ttactacgca ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc
    34681 cggggtgatt tccagaatac gggtcgccag ggagcttaca aactcacggt cgtgagaaac
    34741 aaagatcagc gtgccctgat acagttccag cgccatgttc agcgactcaa tggattccat
    34801 atccaggtgg ttggtcggtt cgtccatgat cagaatgttc ggcttctgca tcattaactt
    34861 accaaacagc atccgccctt tttccccacc ggaaagcact ttagctggct ttttgatgtc
    34921 gtcctggctg aacagcaaac gaccgagaat actgcgcacc gcctgctcgt catcgccttc
    34981 ctgcttccac tgactcatcc attcgaacac ggtcagatca ttttcaaact catattcgtg
    35041 gtcctgagcg tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc cgctgtccgg
    35101 ttgcagatcg cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag
    35161 taccgccagt ttttcaccca cttccagcag caggttgaga tttttaaaca gtggaccgtt
    35221 atcaaaccct ttagtcagac cttccacttc tagcgcgtta cggaacagtt tcttatcctg
    35281 ttcaaaacgg atgaacgggt tctgacggct ggaagctttc acctcttcca gtttgatttt
    35341 atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg
    35401 gctaacgaaa gattgcaact cagcaatctg cgctttcttc ttcgcgttat cagccagcag
    35461 acgttcacgc gcctgagtcg ccgccgtcat gtactcatcg tagttgcccg gataaacgcg
    35521 cagctcgccg taatccagat ccgccatgtg ggtacaaacc atgttaagga agtgacggtc
    35581 gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc acctgttcca gccagcgaat
    35641 ggtgtcgata tcgaggttgt tggtcggttc gtcgagcagg agaatgtcgg gatcagcaaa
    35701 cagcgcctga gccagcagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc
    35761 gtagtgctgt tccactggaa ttcccacgcc aagcaacagt tcaccagcgc gagcttccgc
    35821 agagtaaccg tccatttcgc cgtatttcac ttccagatcg gccactttat agccgtcttc
    35881 ttcactcatt tccggcaaag catagatgcg gtcacgctcc tgcttcactt cccacaactc
    35941 tttatgcccc atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg
    36001 acgcagctta ccaatgcgtt cgttgggatc gagggaaacg ttacccaacg tcggctctaa
    36061 gtcgccgccg aggatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc
    36121 gtaacggttg ccgccgccga atttgacgga aatgttttca aacaacggct tactgccgaa
    36181 ctgcatggtg acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac
    36241 cgcgcattat gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa
    36301 gccaaaggca aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact
    36361 tataatgcgc ttccagtact ctaatattct caacccaatg gcctgccagg cacaaaatct
    36421 cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct
    36481 tttgcagtac cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg
    36541 gtcagaatca ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg
    36601 ccgagtacca gatgggactt tccaatatga tggaagcgaa cccgggtgtg gataccttcc
    36661 tgccgaaagg cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc
    36721 atgaaggcat cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca
    36781 acaccgttat cgtgctgccg atcggcattg gtcagttagg caaagacacg cctatcaact
    36841 ggaccaccaa agttgagcgt aaaaaagcag gcccgacctg gacgccgacc gctaaaatgc
    36901 acgcagagta ccgcgctgcg ggcgaaccgc ttccggcggt cgttccggct ggtccggata
    36961 acccgatggg gctgtatgca ctctacatcg gtcgcctgta cgctatccat ggcaccaacg
    37021 ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca
    37081 tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg
    37141 taaaagcgac caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta
    37201 ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat taccctgacg aagagcgtgc
    37261 agacagtgac cggtcagcca gatgttgacc aggttgttct ggatgaagcg atcaaaaatc
    37321 gttccgggat gccggttcgt ctgaattaat cttcaaaacg taaagcaaag gcggactcat
    37381 aatccgcctt tttaatttga cagaccttat cccgccggga tcataaccaa caaaacatat
    37441 cccaccagcg ccgcccacaa cagcatcggt atcgaataaa gatgaaaacg ccaccagata
    37501 cggcgatcgt tcgccatccg taacgcaatc aaatttgcca gcgatccggg taataaacca
    37561 aagccaccta cgtttaccgc ccatgccagt aataaagacg gtggcacata attgagcagc
    37621 aaaatggtac tcggcacgtt actgatcacc tgcgataaac cgatcgccgt tagccataat
    37681 ccgggttcgg ataaatgact cacgttactt aacaccccct gcaaaactgg cagttgggtt
    37741 agtaaatgga cgtcgataaa catcgccata aacaccagca gcagcgtcca gtccacactg
    37801 agcaccacgc gacgcgccag cagcgcaaag cccgccgcca caatcaccag tccccacagc
    37861 tcttgtttta actccagcgc cgtcagaaag acgatataca gccccagaca actccacacc
    37921 agccgcggtt tccactccgg cgtttgccct cctgtatggt actgcagcgc ctttccaggg
    37981 aaacaacacc agcacaacag caggagtgtc agcatcattg cgccagccag cggtgccatt
    38041 tgggcaataa atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg
    38101 ttgccaattg gcgtcagtag cgaaccagca ttgactgcca gcgcctcaaa aataatcagc
    38161 cgattaaccg ggatctcaca cagtcttttt agcgtgatag tcagcggaac aactataaac
    38221 agcgcgacat cgttggtcag aaaggtagaa agcagcgccg ccgccagtac cataaacatc
    38281 gccagccgac gctccgtagc aaaacggcgc accattttgc gccccagcac atcaaaataa
    38341 ccgcttaact cgacaccttt agtcagcagc atcaggccgc ttaaggtgat gatggtgtgc
    38401 cagtcgatag cagcaggcca ggatttcggt gcaaagggaa cgaaaaaact taatccgatg
    38461 ccaacaagaa ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg
    38521 ctcatttggc accgtgtttc tgggtaaaca aacgaaaggc atccagcgtc tcttcactaa
    38581 catggtgctc cataccttcc gcgtcgcgac gggcgatttc cggactgacg cccaacacca
    38641 acaagaaatt ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt
    38701 ctgccgttaa aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagtc
    38761 gcttaagcat tttagccacc gtcggttgcg aaacccccag acgagcagcc atgtctacct
    38821 gacgagcttc ccccacttcc ctgatcaagt cagaaatcag ctcaacataa tcatcaataa
    38881 gctcgcgccg atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca
    38941 ctaactgcgt cacttttttt gctgttggcg tacctgcgcg acgactcatt atgcttcctc
    39001 attatggtga cgcatacata gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg
    39061 aaggtacaaa aaattaacgt tttagcaata gctatataat atagcctgtg ctatatctgt
    39121 atgtaatgca atcatccctc aaggatcgat gggattagca agtcaggagg tcttatgaat
    39181 gagttcaaga ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatgctg
    39241 ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc aggataaacc ttccgataaa
    39301 tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg caggcacaac gcgttttggt
    39361 ctgaaaaacc ccactttttg tcggatttgc aatccccttc gcaaaagatt tgttcgtcgg
    39421 tagttgacct gaacagcagc tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg
    39481 cggatgcgga cccctttccc ctcttcacgc actcttgcag gtattgaccc ttgacgccag
    39541 ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct
    39601 tgttacccgt agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt
    39661 aattaacctc ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat
    39721 tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtatcagttc
    39781 gctggtggcg gcttgttatt tcccttttgc tcaggcctac ggcgcgccta acttcaatac
    39841 attgctggca ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc
    39901 gtggtacagc tacctggtcg gcatatttat ttttgcgctc ggcgtaatag caatcaggcg
    39961 aaaaaaagag agtgaaaaag cgcgctggaa taccttcgac agcctgtgcc tggtattcag
    40021 tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc tggggcggcg tatttaaact
    40081 gaaagatact ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga
    40141 ggtgattgaa gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt
    40201 cactgccgtt aagccgaagt atcagaccta tgtggtggtg atcggagaaa gcgcgcgtcg
    40261 cgatgccctc ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa
    40321 cggtttgata tttgctgact acattgccgc cagtggctcc acgcagaaat cgcttggctt
    40381 aacgctcaat cgcgttgtcg atggcaaacc acagtttcag gataactttg tcaccctggc
    40441 aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag ggtcaaatcg gcgaatacga
    40501 taccgccatc gccagcatcg ccaaacgagc agatgaagtg tacttcctga aagaaggtaa
    40561 ttttgaagca gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct
    40621 ggcgcaagag cactcgcaac cgcagctgat tgttctacat ctgatgggct cgcatccgca
    40681 ggcctgcgac cggacacaag gaaaatacga aacctttgtg caatcgaaag aaacgtcgtg
    40741 ctatctctat accatgacgc aaacagacga tttactgcgc aagctgtacg atcagttacg
    40801 caacagcggc agcagcttct cgctggttta cttttctgac cacggtctgg cctttaaaga
    40861 gcgcggcaaa gacgtgcaat accttgctca tgatgataag tatcagcaaa atttccaggt
    40921 gccttttatg gtcatttcca gcgacgataa agcgcatcgg gtgattaaag cccgccgctc
    40981 ggccaatgac ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa
    41041 tatcaaatac ccgtttatat ctgaaaagaa agccgggccg atatacatca ccaacttcca
    41101 gttacagaag gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa
    41161 caaaaaaatc cgccccgaga ggcggatttt tatatcaccg aagtgattag aagcggtaac
    41221 caacaccggc aatccaggtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga
    41281 agtccagagc aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt
    41341 agtcgctggt gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca
    41401 cacccactac accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga
    41461 tgccgtagta ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt
    41521 aagtgaaaga accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt
    41581 tgaaaccgcc cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta
    41641 cagtagaagt cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg
    41701 aaagacatgc aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt
    41761 aaatatatca aaaattaatg ggaaactctt cgcgatttat gatgtctaac gggccatttc
    41821 atgtaacaga acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga
    41881 actgcgtaag tattacgcat attttccctg tcattcatcc agattattcc taatcaccag
    41941 actaatgatt ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca
    42001 aatgctcaac cagcattggg tatatccagt acactccacg ctttacttag gtttagatat
    42061 ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca
    42121 tattgctcgt tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc
    42181 ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc attaggcacg ctgatcctca
    42241 tcgcgttctt taagccatgg cgactgcgct ttgccaaaga gcaacgatta ccgctgttgt
    42301 tttacggcgt ttcgctgggt ggaatgaatt atctttttta tctttctatt cagacggtac
    42361 cgctgggtat tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt
    42421 ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc
    42481 taccgctggg gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg
    42541 ccggggcttg ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc
    42601 ctgcgacggt ggcaattggt tcgttgattg cagcgttaat tttcgtccca attggagcgc
    42661 ttcaggctgg tgaagcactc tggcactggt cggttattcc attggggctg gctgtcgcta
    42721 ttctctcgac cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac
    42781 gcacatttgg tacgctaatg agcatggaac cggctctggc tgccgtttcc gggatgattt
    42841 tcctcggaga aacactgacc cccatacagc tgctggcgct cggcgctatc atcgccgctt
    42901 caatgggttc tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacataaatt
    42961 aaatttacat ttctgcatgg ttatgcataa ccatgcagat tttctcgcta cttttcctct
    43021 acaccgtctt tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag
    43081 caaataaata agaattgttc ttattaatat atctaactca ttgatttttt tattgttttt
    43141 gtttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag cggaacacat
    43201 agctggtgct atacttagtc tcgttaatta ctgggacata acatcaagag gatatgaaat
    43261 tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata cccgcaacga
    43321 tgtctctgac agcgagaaaa aagcaacagt agagttgctg aatcgccagg ttatccagtt
    43381 tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg ctaacttcat
    43441 tgccgtacat gaaatgctgg atggcttccg cactgcactg atcgatcatc tggataccat
    43501 ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg accactcaag ttatcaacag
    43561 caaaactccg ctgaaaagtt acccgctgga catccacaac gttcaggatc acctgaaaga
    43621 gctggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg gcgaagcgaa
    43681 agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata aattcctgtg
    43741 gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt tagtacacga
    43801 taaaagcggc ttcctgacag gaggccgttt tgttttgtag cccgcctcaa cgcacttatt
    43861 tagtgcatcc atctgctatc cccagctggt taagtaaatt ttttgtatcc acatcatcac
    43921 acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat cactgtgttg
    43981 agtgcacaat tttagcgcac cagtttggtg ccccagaatg gtgcatcttc agggtattgc
    44041 cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt tgcaaaactg
    44101 gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga tagaaaaaag
    44161 gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg gcttttgcgg
    44221 tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc ttcgttccgt
    44281 ttgaatttaa acagggcgat aaatacgtgg gctttgacgt tgatctgtgg gctgccatcg
    44341 ctaaagaact gaagctggat tacgaactga agccgatgga tttcagtggg atcattccgg
    44401 cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc gacgagcgta
    44461 aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg atggtgaaag
    44521 ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt gctgtgaaga
    44581 gcggcactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat ctgcgtcagt
    44641 tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac gccgttctgc
    44701 acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag ttcaaagcgg
    44761 taggtgactc tctggaagca cagcaatacg gtattgcgtt cccgaaaggt agcgacgagc
    44821 tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact tacaacgaaa
    44881 tctacaaaaa atggttcggt actgaaccga aataataacg ctgcgcctgt aaagcgcact
    44941 ggcagttccc tctccccgcc ggggagagga ttagggtgaa gggcgcaaac ccgctccggg
    45001 gccattaatt accctgaatt tgattattta caccacggta acaggaacaa catatgcagt
    45061 ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc aaaatgaccc
    45121 tgtggatttc ggtcctcggt ctggctggcg gtctggtgat cggattgttg gcaggttttg
    45181 cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt gaagtgatcc
    45241 gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg atggcgttta
    45301 acgatttacg catcgacccg tttactgcgg cggtggtcac catcatgatc aactccggcg
    45361 cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat tcacaaaggt tttcgtgaag
    45421 caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt ttaccgctgg
    45481 cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt aaagacactt
    45541 cgctgtttat tgttatcggc gtggcggaac tgacccgtca ggggcaagaa attattgccg
    45601 gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg attattaccc
    45661 tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg tgattgaatt
    45721 taaaaacgtc tccaagcatt ttggtccaac ccaggtgctg cacaatatcg atttgaacat
    45781 tgctcagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat cgaccctgct
    45841 gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg atggcctgaa
    45901 ggttaacgat ccgaaagttg atgagcgcct gattcgccag gaagcgggta tggtgttcca
    45961 gcagttttac ctcttcccgc atctgacggc gctggaaaac gtcatgtttg gcccgctacg
    46021 cgtgcgtggc gcgaataaag aagaggcgga aaaactggcg cgtgagctgc tggcgaaagt
    46081 cggtctggca gagcgtgcac atcactaccc ttccgaactt tctggtggtc aacagcagcg
    46141 tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg atgaaccgac
    46201 ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg atctggctga
    46261 agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga aagtagcttc
    46321 gcgcctgatc tttatcgaca aaggccggat tgcagaagat ggcgatccgc aggtgttgat
    46381 caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt aacttctctg
    46441 cctccctttc ccccgaaagg gaggcacacc agattcctct catttaaaat tgcctctccc
    46501 ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc
    46561 ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat acccggcgtt
    46621 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
    46681 aaaaaagcgg cctatggcgc actggcggat gtgctggata atgacacctc gcgtaaagag
    46741 ttgatcgacc agttgcgcac cgtcgccgcg acgccccctg cggaaccggt accgaagatc
    46801 gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc
    46861 cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat caccggctcc
    46921 ccgcataagc cgtttaatcc acaaaccttc agcaatgcgc tgacccactt ttcaatgtta
    46981 gcggtattag tgtttggttt ttactggctg attcgtctgt gcgcactgcc gctgtattgc
    47041 aaaatgggcc agtgggcgcg gcaaaaaaat cgtgagcgca gtaactggtt gcagcttccg
    47101 gcgatgatca tcggggcgtt tattatcgac ctgctgttac tggcactgac attatttgtc
    47161 ggccaggtat taagcgacaa cctgaatgcg ggcagtcgca ccatcgcttt ccaacaaagt
    47221 ttgtttctca acgcctttgc cctgattgaa tttttcaaaa ccgtactacg cctgattttt
    47281 tgcccaaacg tagctgaact gcgcccgttc acgattcatg acgagaccgc ccgttactgg
    47341 agtcgtcgcc tgagctggtt aagcagcctg attggctatg gtctgattgt ggctgtgccg
    47401 attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg
    47461 tgcatgaccg tctgggcgtt gtacctgatt tttcgtaata aaaaagagat cacccagcat
    47521 ttgctcaact ttgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgca
    47581 ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc
    47641 gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt
    47701 ggtatcgcag catttgtttc cggtatgttt tcccgctggc tggcgaaaac catcaccctc
    47761 tcgccacata ctcagcgaaa ttatccggag ctgcaaaaac ggctgaatgg atggctttcg
    47821 gcggcactga aaacagcgcg tattctgaca gtctgcgtgg cggtaatgct gctgttgagc
    47881 gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gtgcggggca gaaaaccgta
    47941 gatatcctga tccgtatcgc actcattctt ttcttctcgg cggttggctg gacggtgctc
    48001 gccagtttga tagaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc
    48061 cgtacgcgta ccctgctaac gctgttccgt aacgcgctgg cggtgattat cagtaccatc
    48121 accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc
    48181 ggtgcattgg gactggctat ctcttttggt tcgcaaacgc tggtgaaaga tattatcacc
    48241 ggggtattta ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg
    48301 ttgaccggca ctgtggaacg gatgtcaatt cgctccgtgg gcgtgcgcca ggataccggg
    48361 gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt ccgcggtatt
    48421 ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa agccaatcag
    48481 gcactgaaag atgcggtagc agaattgatg gaaaacgaag aaattcgcgg gctggttatt
    48541 ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt
    48601 tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg
    48661 aaaaaacatt tcgacctggc gggtgttcgc gcgccggtgc agacttatca ggtgctgcca
    48721 gctccgggcg cggccccggc tgaaccgttg ccgccggggg agccaacgct ttaacgctgg
    48781 cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggctat aaagcggctc
    48841 tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcacctac gtccgtcaaa
    48901 gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa ccacatcacc
    48961 tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaagcgac ctcaccgcct
    49021 tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt gttcagcccc
    49081 aggttacggc gtttacgttc actacctgcc cgtgccgcag cggcggaatc gtggaatggc
    49141 gggttacaca aggtcgcatc gtattgctcg tttttatgga tgatgccgtt aaaaatcgcc
    49201 ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt agcactgatg
    49261 atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa acgccagcca
    49321 tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg
    49381 ctggcattag ctggaattgt tccgcttgct tcggccagta aatcggcaag gtgatgaata
    49441 taatccgccc ggcccggtac tggtgggcag agaaaaccgt ccgggatatc ccagttcgct
    49501 acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca
    49561 aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag ttcaggattg
    49621 acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc
    49681 ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc cgcataacgc
    49741 gacagggtaa atatctggct gcgtttatca tttctaaaaa ccttcacatg gctatggttt
    49801 atggcaattc accataaaaa acatcatcat gcatgtcatt gatttgtctt attttgtaaa
    49861 aacacatcgt tcaaaaaata agcgcatctc tgtcaatttt ttgcgtcatt ttcatcgcgc
    49921 aggcgaccta cacttatctg gcactacccg ttgtatttat gaggatgtca ttatgaaaaa
    49981 gtgcctcaca ctactgattg ccaccgtcct gagcggaatc tctctcacgg cttatgccgc
    50041 gcaaccgatg agtaacctgg acagtggtca actacggccc gccggtaccg tttcggcaac
    50101 tggcgcatca aacctaagcg atctggagga taaactggca gaaaaagcgc gcgaacaagg
    50161 cgcgaaaggt tatgtcatta attccgcagg cggaaatgac cagatgttcg gtactgcaat
    50221 catctacaaa taaccccacc aacgtaactg cattcgcctc tggatgcagt tttcgtccaa
    50281 tttgtaagca aaaaacatca aatatgaacg caatgtaaat aaatgtattt ctttttcgcg
    50341 caatgggtga tagaaaatcg cgccaagtga taatgcttat caatattatt atcacttgca
    50401 cgtacgctat cacgggatta acagtggcat cgcatccgca gagatgcttt ctcgtgacag
    50461 tgaaaatttc accatataag aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc
    50521 tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggta ttacgcctgt
    50581 ggcacaggca ctcgctgccg aagggcaagc taacgcggat gacacgctgg ttgtcgaagc
    50641 atcaacgcct tcgctttatg cgccacaaaa atctgccgat ccgaaattct cgcgtccggt
    50701 agcggatact acccgcacga tgacagtgat ttctgaacaa gtgattaaag atcagggtgc
    50761 aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga
    50821 gaacggtaac tccaccactg gcgacgccat ttatatgcgc ggtgccgata cctctaacag
    50881 tatttatatc gatggcattc gcgatatcgg cagcgtctcg cgcgacacct tcaataccga
    50941 gcaggtcgaa gtaattaaag ggccgtccgg caccgactac gggcgcagcg caccgacagg
    51001 ctcgatcaat atgatcagca aacagccgcg caatgattcc ggcattgacg cctccgccag
    51061 tattggcagc gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac
    51121 cactgcggtg cgcctgaatg taatgggcga aaaaacgcac gatgccggac gcgacaaagt
    51181 caaaaatgag cgttacggcg tcgccccttc tgtcgctttt ggccttggta cagcgaatcg
    51241 tttgtatctt aattatctgc atgtcaccca gcacaacacg ccagacggcg gcattccgac
    51301 catcggttta ccgggttttt ctgccccatc tgcgggaaca gcggccctga atcattccgg
    51361 aaaagttgat acgcataact tttacggcac ggattccgat tacgacgatt cgaccaccga
    51421 caccgctacc atgcgttttg agcacgacat caacgataac accaccattc gcaacactac
    51481 gcgttggtcg cgcgtaaagc aggattacct gatgacggcg attatgggtg gagcgtcgaa
    51541 tattacccag cccaccagcg atgtgaatag ctggacgtgg tcacgcacgg cgaataccaa
    51601 agatgtgagt aacaaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg
    51661 cgctatcggt catgatgtca gtaccggcgt ggaatttacc cgcgaaacac agactaacta
    51721 tggcgttaat ccggtgacgt taccgacggt aaatatttat catcctgaca gcagcattca
    51781 tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat
    51841 ttatgccttc gatacgctgc aaatcacccg tgattttgag ctgaacggtg ggatccgtct
    51901 ggataattat catactgaat atgacagtgc caccgcctgc ggcggcagcg ggcgcggtgc
    51961 catcacctgc cctgctggcg tggcaaaagg ttctccggta accaccgtcg acaccgccaa
    52021 gtcgggcaat ctggtgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggcaa
    52081 tgtttatatt aactatgccg tttcccagca gccgccgggc ggcaacaact tcgcccttgc
    52141 gcagtctggc agcggtaaca gtgccaaccg caccgatttt aaaccgcaaa aagccaacac
    52201 cagcgagatt ggcaccaaat ggcaggttct ggataaacgc ctgctactta ccgccgcgct
    52261 gttccgtact gatattgaaa atgaagttga gcaaaatgat gatgggactt attcgcaata
    52321 tggtaagaaa cgtgtcgaag gctatgagat atccgtggcc gggaatatca cccccgcgtg
    52381 gcagatgatt ggcggctata cccagcaaaa agcaactatc aaaaacggca aagatgttgc
    52441 ccaggatggt tcctcatcgc tgccgtatac cccggaacac gccttcacct tatggagcca
    52501 gtatcaggca accgatgata tttctgttgg tgcgggcgca cggtatatcg gcagtatgca
    52561 taaaggttca gacggcgcgg tgggaacgcc agcatttacc gaaggttatt gggtcgccga
    52621 cgccaaactg gggtatcggg ttaatcgcaa tctcgacttc cagctaaacg tctacaacct
    52681 gtttgatacc gattacgtcg cctcaatcaa taagagcggc taccgttatc acccgggcga
    52741 gccaagaacc ttcttgctca cagccaatat gcatttctga ttcagatgtg gggcgcaggc
    52801 cccacttttt ggagaacttg tatgatgtac cacattcccg gcgtgttatc gccacaggac
    52861 gtcgcccgtt ttcgcgaaca cctggaacaa gccgaatggg tggatggacg cgtcaccacc
    52921 ggcgcacaag gtgcgcaagt taagaacaat caacaagtag acacccgcag cgccctgtac
    52981 gccgccctgc aaaatgaggt gctgaacgcg gttaaccaac atgctttgtt ctttgccgcg
    53041 gccttgccgc gtaccctttc cacgccgctg tttaatcgct atcagaacaa tgaaacctat
    53101 ggttttcatg tggatggcgc agtacgcagc catccacaaa acggctggat gcgcactgac
    53161 ctttctgcca cgctgttttt aagcgatcca gaaagctacg acggcggcga actggtcgtt
    53221 aatgacacct tcgggcaaca tcgggtaaaa ctcccggcag gcgatctggt gttgtatccc
    53281 tccagcagcc tgcattgcgt gacacccgta acccgcggcg tgcgagtggc atcatttatg
    53341 tggatccagt cgatgatccg cgatgataaa aagcgcgcca tgctgtttga actggacaac
    53401 aatattcagt cgctgaaaag ccactacggc gaaagtgaag agatcctgtc gctgctcaat
    53461 ctttatcata atctgctgcg ggaatggtcg gagatctgac gcttgaactg cccgagttcg
    53521 tccctatatt agctacactt aactgtacaa gcattgatat ggggaggacg atatggcatc
    53581 cggttgggct aatgatgacg ccgtcaacga acagatcaac agtacaattg aagatgcgat
    53641 tgcccgcgct cggggtgaaa ttccgcgtgg cgaaagcctg tatgaatgtg aagagtgcgg
    53701 tgcccctatc ccgcaggccc gtcgggaagc cattcctggc gtgcgcttat gcattcattg
    53761 tcagcaggag aaagatttac aaaaaccagc ttatacagga tataatcgca gaggttcgaa
    53821 agacagccag ttacgttaac tacaactggc gggatgtata gcgaatataa tttgtatttt
    53881 gcgtggcatt ccgtgccttt aacgctaccg tgactgccac gcggtataaa acaagtccat
    53941 aagtacaaca aataaatggt ttatcagtat gttagatatc aatcaattta tttgaacaag
    54001 gtggtcaatt ctcttcgatt ttatctctcg taaaaaaacg tgatactcat cacatcgacg
    54061 aaacaacgtc acttatacaa aaatcacctg cgagagatta attatgaaaa ctatcaatac
    54121 tgttgttgct gctatggctc tttcaactct gtcatttggc gtgttcgcgg cggaaccggt
    54181 aacggcatcc caggcacaga acatgaataa aatcggcgtg gtttctgccg atggcgcatc
    54241 caccctcgat gccctggaag cgaaactggc tgagaaagcc gcggcggccg gtgccagtgg
    54301 atacagcatc acttctgcca ccaacaacaa taaattaagc ggtactgcgg taatttataa
    54361 gtaattcgta agctggatca ggcgtttacg ctacgttcag cacaagtatt tccctgtctg
    54421 tttgccgaca gacgcatatg ctctaaccct catgatccta tgttaccctt gtttgcccgt
    54481 ccgccactgg acgggctttt tttagctggc tagctgctga cagaaagcct gcaacgtctc
    54541 ttccggcata ccaatctgcc gcgccgcatc acaaatggcc tgccagcttc ccgcatccag
    54601 tggaatcccc tgctcctgcc gttcgcgacg cgtgttcact tcccactcgc ccggtagcaa
    54661 aatcggctta tcgtcatcat gcggcgaggc tttcacccac tcggcaaagg cttcggtctg
    54721 cgcactacaa tccggcgcgc cgaacagttc cgggttgata atgatagtgg tcatgcagtt
    54781 aagaatggca tcgggactgg tttgtaacgt ttcctgatgc gtcgttttac cgccagaaag
    54841 cgccccgcca agaatttcac acatcgctgc aagggcgtag cctttatgtt cggcaaaggt
    54901 caacagcgaa cccaacggcg actcctgcat taccgccgga tttgtcgtcg gcacgccgtt
    54961 aacgtcaatc aggcaacctg gcggcacggg gacgccttta tgccaggcga cgcgggtttt
    55021 gccaaatgca atggcgctgg tggcgtaatc gagcaacagc gggaaattat ctttacgagg
    55081 gaaaaccaca cagaacggat tggtgccaaa gcggctgtcg cgaccgtgga acggcgcgac
    55141 cattggaata ccgaccacgc taacaaagtg gatagagaca aaccccgccg ctgcacactg
    55201 ctccgcccag taaccgatac ggccgatatg atgcgagtta tgtagcgcca cggcggcaat
    55261 gccgtgctga tgcgctttct caatccccag cgccatcgct tcgtgtgccg cgacctgacc
    55321 aaatgcgcga tcgccatcga gcgtgaccgc cgcccccgct tctttaacga ctttggcatg
    55381 atggttaatt tgcagatgcc cctgactcca ggagcgcaca tagcttggga tcatgccaat
    55441 accatgtgaa tcatgccctg ccaggtttgc cgcgatcaaa tgatcggcga ctaatttcgc
    55501 ttcctgttcc tcactaccca tctgacgaaa tacagcctga ataaaactgt gcagcgtctg
    55561 agcatcaaag cgatggccac tttccatggt taactcctgt ttatgttatg tgtttgttgt
    55621 tttttatgct gcacgccggg cgcgggaagg tcaataaaga tgcaggaatt tcatattgcg
    55681 gtggggtaaa agccggatga catggctcat ccggctgaga taaaaattaa aatgcctgat
    55741 taacgcgcgc caggccgtcg ctgatagttg ccgcttcacc cgtagccatc aggcagcaag
    55801 ccatctggat tttcagcgat tcgggaatcg gttcgctgcc agccaggcaa cgctcaatcc
    55861 actgcgccgt ggtttccgga tcttttgctt gtggcagtaa ctcgctacca gcagtgtcct
    55921 gtttttcata cagtacccgc atcccttcac ggtcaatgag attgatctgc gggcagcgct
    55981 gcggattagc atacacttca ccttcagtac catgcataag tagcgcccgc ccgccgatat
    56041 cgctaaagaa cttcgcgacg cgtccaatgt attccggatg cgaaacgctg gaaagacgca
    56101 gtgcctcacc ttcggcaaat ggcgtcgcca gtttcgccag ggtatgtgca ctgttacgca
    56161 cgcccatccg ccagcgcatc gccagttgtt tttccagcgg cgggcaaaac gcgccgacag
    56221 gcataaagac aggttgatgt tcgtcgagct tcgcctgcgc ctgcccgccg tgcaacgttg
    56281 gcgtaatacc catcagttcg aaaatggttt cggtcagcac gcgggttgga tcttcgctaa
    56341 ccccgtgaac caccacagga aaaccgagtt tgtgcagcag aatcgccagc aacggcgtca
    56401 ggttggcctg tttacgcgcg ccgttgtaac tggggatgac aatcggcatc ggcttgcctg
    56461 caggcggcgt cagcttgatg gtgtgatttt gcatggcttc gtaaaagccg agcatctctg
    56521 cttccccttc ccctttgata cgtagcgcaa tcaatacgcc gcccaactca aggtcaggga
    56581 cttcaccatt gagcatatgg gcatacagac cgcgcgcagt atcccggtct aaatcgcgcg
    56641 cgtggttttt cccgcgcccg atctctttaa tgattttgcg atagtccatt tacgactcct
    56701 tacctgactc acatcattaa cgccgccgac ggcgtgggga tttcgttttt tctttctttt
    56761 taactatacc ttcagggact tccggttgct ctatcggaaa taccggtaat gcatccagta
    56821 gtcgcttgcc ataatttttt gtcagcaagc gtttgtcgta gataaccact tccccccagc
    56881 aaccgtggct tcgaatcagt cgcccaacct gctgaatcag gttaaacgaa gcgctcggca
    56941 ggctttgcac ctcaaacgga tagcggttaa ggcttttcag ccattcacct tcggtgatca
    57001 ccaccggact gtcgatgggc ggaaaagcga ttttgtggat atgcacctgg ctgagcagag
    57061 cgcctttcag atcaagacct tcggcaaagg actgtaagcc caccagcacg ctacgctcac
    57121 cgttggcaac gcgtttgcgg tgcagttcaa ctaagcgata acgtggctga tcgccctgca
    57181 ccagcaacat caggcgtaaa tccgtcacat agtcgagaaa gcgctgcatc gcacgcccgc
    57241 tggcaaacag caccaacata ccgagatgtt ttttgctctc cacctgctca cggaaaaagg
    57301 ccgccatttc agcaatgtgc tgttcttcgt tgtcgatgga aggctcaacg cgcatccggg
    57361 gaataacaat tttgccctgt tcgcagtggt taaagggcga atccagcgcc acaaagcggt
    57421 cacccgcttt ctctttcagc ccactcatct cctgcaaacg cgaaaaactg ttcagcgaac
    57481 gcaaggttgc ggaggtaaca ataatgtgcg gaatactgcg ccacagcagc ctttccagtt
    57541 ggtcgctaac acgaataccc acacagtgaa accacagatg tagctgccct tcgcgctctt
    57601 cccgcgtcgc ccatttggtc accggcgcac cggaagattg cgccagagac gccagccgcc
    57661 agagtttgct ttgcgcctcg aacatcccca acgcgcggtt catctgcaaa atcaaccgat
    57721 gcaggcgtac aatgtcatgg ctgccggttt tctcacttaa atcgttaaga aataactccg
    57781 ccagaccacg cagcatctcg gtgagttttg ccagccgctg gcagatctcc agcacttcat
    57841 caggcagttc gcccatcgca aaacggtgct ctgcctcctg cccggcaggc atgtagagat
    57901 tgagaatgtt gtttaacgag gcgataagct catacagctc ttcacaatgc gcattcaaac
    57961 gttcagggat cgccagcggt gggatggtct tcgggcgaaa ctgctccatg caggtggcga
    58021 ccagtttggt gaacaggtcc agttggagcc ggtaccaggg cgcagtgatt tcggcgctca
    58081 tctccagcgc atcccgcgcc acatccggca ggtgatgacc ttcgtccagc accagcagta
    58141 aatttttcgg gtcaggcaat accgcttcgc tttccattgc cgccatcacc agcgcatggt
    58201 ttgccaccac cacttccgct tcctgaattt cccgacgagc gacaaaaaac gggcattcgc
    58261 gatagtagta acagttgcgg ttgaggcagc tggctttgtc ggtactcaaa cgacgccaga
    58321 gatcatcatc tatagcgata tcagtatgat cgcgcaggcc atcccattta taagtgtcga
    58381 gatcgccctt cagcttcgca caacgttttt gctcttcctg attgttcggc gtcagttcgt
    58441 cgtcaagaaa cgccagcaga tcctgttgcg tgggctcagt gctggcgagc gctgtcagat
    58501 tacgcgggca aacgtagcgc ccacgcccaa aagcggcagt gaatttaaga tcgggaatga
    58561 tctttttcag cagcggtaaa tctttgctgt agatctgatc ctgcaatgcc acgttggcgg
    58621 tacttaccac cagcgttttt tgctcttcgc gggcaatggc gatgccgggg atcaaatagg
    58681 agagcgtttt cccaacgccg gtaggagctt caatcgccag atgccgccct tcttctccgg
    58741 ccagcgtttt ggcgacgtcc gcaatcatct gccgctgcgg cgcacgggga ataaagtcgg
    58801 ggatctgttc ctgaagcgcc ttataccagg cggcaatttg cgctttaagc gcggcggtta
    58861 atgccatgag aaaacctgaa atactgtata aacagccaat attgtggcat ttttcggttt
    58921 tcaggggtaa tggtttttct gtttctggaa agcggctcgc aataaccagc aggatgatgc
    58981 gtaaagcgct tcgcgctaaa ttcgaacaac atgcagaact gcgtacactc ttgctggcaa
    59041 ccgcgtccgc aaaactggtt gagcatacgc aaaatgatgc ttactgggga gatggcggta
    59101 atggtcaggg caagaatcgc ctggggtacc ttttaatggc gttgcgcgga caattggctg
    59161 cggagaagta acgcatcggg tggcttgtgg gcgttaaaac agagaaatct ttttgccgcg
    59221 ctcgcaaatg gctgacaagt gcttgttgtg aaatgccgga tgcggcgcga gcgccttatc
    59281 cggcctacaa aaacatgcac attcaatata ttgc
//
LOCUS       DAOFSV010000012        55712 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.012, whole genome shotgun sequence.
ACCESSION   DAOFSV010000012 DAOFSV010000000
VERSION     DAOFSV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.012"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1258)
                     /gene="rsxC"
                     /locus_tag="QBH27_000833"
     CDS             complement(<1..1258)
                     /gene="rsxC"
                     /locus_tag="QBH27_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDQ7013189.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIEC"
     gene            complement(1251..1829)
                     /gene="rsxB"
                     /locus_tag="QBH27_000834"
     CDS             complement(1251..1829)
                     /gene="rsxB"
                     /locus_tag="QBH27_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDQ7013190.1"
                     /translation="MNAIWIAVAAVSLLALAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(1829..2410)
                     /gene="rsxA"
                     /locus_tag="QBH27_000835"
     CDS             complement(1829..2410)
                     /gene="rsxA"
                     /locus_tag="QBH27_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDQ7013191.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2487..2927)
                     /locus_tag="QBH27_000836"
     CDS             complement(2487..2927)
                     /locus_tag="QBH27_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDQ7013192.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3013..3228)
                     /gene="ydgT"
                     /locus_tag="QBH27_000837"
     CDS             complement(3013..3228)
                     /gene="ydgT"
                     /locus_tag="QBH27_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDQ7013193.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(3500..3625)
                     /gene="blr"
                     /locus_tag="QBH27_000838"
     CDS             complement(3500..3625)
                     /gene="blr"
                     /locus_tag="QBH27_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDQ7013194.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            3868..4908
                     /locus_tag="QBH27_000839"
     CDS             3868..4908
                     /locus_tag="QBH27_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ7013195.1"
                     /translation="MSDNIRVGVIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKTLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(4944..5945)
                     /gene="add"
                     /locus_tag="QBH27_000840"
     CDS             complement(4944..5945)
                     /gene="add"
                     /locus_tag="QBH27_000840"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDQ7013196.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLT
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6049..7221)
                     /gene="malY"
                     /locus_tag="QBH27_000841"
     CDS             complement(6049..7221)
                     /gene="malY"
                     /locus_tag="QBH27_000841"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDQ7013197.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(7231..8823)
                     /gene="malX"
                     /locus_tag="QBH27_000842"
     CDS             complement(7231..8823)
                     /gene="malX"
                     /locus_tag="QBH27_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDQ7013198.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTMDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            8998..10026
                     /gene="malI"
                     /locus_tag="QBH27_000843"
     CDS             8998..10026
                     /gene="malI"
                     /locus_tag="QBH27_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDQ7013199.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVIWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            10138..10905
                     /gene="hdhA"
                     /locus_tag="QBH27_000844"
     CDS             10138..10905
                     /gene="hdhA"
                     /locus_tag="QBH27_000844"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDQ7013200.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLIAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTNALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGDGVQELN"
     gene            11134..11724
                     /gene="uidR"
                     /locus_tag="QBH27_000845"
     CDS             11134..11724
                     /gene="uidR"
                     /locus_tag="QBH27_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDQ7013201.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            12114..13925
                     /gene="uidA"
                     /locus_tag="QBH27_000846"
     CDS             12114..13925
                     /gene="uidA"
                     /locus_tag="QBH27_000846"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDQ7013202.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQHWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            13922..15294
                     /gene="uidB"
                     /locus_tag="QBH27_000847"
                     /pseudo
     CDS             13922..15294
                     /gene="uidB"
                     /locus_tag="QBH27_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            15333..16599
                     /gene="uidC"
                     /locus_tag="QBH27_000848"
                     /pseudo
     CDS             15333..16599
                     /gene="uidC"
                     /locus_tag="QBH27_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(16644..18152)
                     /locus_tag="QBH27_000849"
     CDS             complement(16644..18152)
                     /locus_tag="QBH27_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDQ7013203.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNLWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(18253..19428)
                     /gene="manA"
                     /locus_tag="QBH27_000850"
     CDS             complement(18253..19428)
                     /gene="manA"
                     /locus_tag="QBH27_000850"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDQ7013204.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            19627..21273
                     /gene="fumB"
                     /locus_tag="QBH27_000851"
     CDS             19627..21273
                     /gene="fumB"
                     /locus_tag="QBH27_000851"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDQ7013205.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILCDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21416..22819
                     /gene="fumC"
                     /locus_tag="QBH27_000852"
     CDS             21416..22819
                     /gene="fumC"
                     /locus_tag="QBH27_000852"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDQ7013206.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(22816..23745)
                     /gene="tus"
                     /locus_tag="QBH27_000853"
     CDS             complement(22816..23745)
                     /gene="tus"
                     /locus_tag="QBH27_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDQ7013207.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSVARFEWVHRHFPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(23821..25122)
                     /gene="rstB"
                     /locus_tag="QBH27_000854"
     CDS             complement(23821..25122)
                     /gene="rstB"
                     /locus_tag="QBH27_000854"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDQ7013208.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(25126..25845)
                     /gene="rstA"
                     /locus_tag="QBH27_000855"
     CDS             complement(25126..25845)
                     /gene="rstA"
                     /locus_tag="QBH27_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDQ7013209.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            25974..26309
                     /locus_tag="QBH27_000856"
     CDS             25974..26309
                     /locus_tag="QBH27_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDQ7013210.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(26306..27028)
                     /gene="folM"
                     /locus_tag="QBH27_000857"
     CDS             complement(26306..27028)
                     /gene="folM"
                     /locus_tag="QBH27_000857"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDQ7013211.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(27065..28447)
                     /locus_tag="QBH27_000858"
     CDS             complement(27065..28447)
                     /locus_tag="QBH27_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ7013212.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(28633..29577)
                     /locus_tag="QBH27_000859"
     CDS             complement(28633..29577)
                     /locus_tag="QBH27_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7013213.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            30101..31633
                     /gene="pntA"
                     /locus_tag="QBH27_000860"
     CDS             30101..31633
                     /gene="pntA"
                     /locus_tag="QBH27_000860"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDQ7013214.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQTAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            31644..33032
                     /gene="pntB"
                     /locus_tag="QBH27_000861"
     CDS             31644..33032
                     /gene="pntB"
                     /locus_tag="QBH27_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDQ7013215.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(33057..34091)
                     /gene="tqsA"
                     /locus_tag="QBH27_000862"
     CDS             complement(33057..34091)
                     /gene="tqsA"
                     /locus_tag="QBH27_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDQ7013216.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            34503..34868
                     /gene="mdtJ"
                     /locus_tag="QBH27_000863"
     CDS             34503..34868
                     /gene="mdtJ"
                     /locus_tag="QBH27_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDQ7013217.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            34855..35184
                     /gene="mdtI"
                     /locus_tag="QBH27_000864"
     CDS             34855..35184
                     /gene="mdtI"
                     /locus_tag="QBH27_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDQ7013218.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(35223..36044)
                     /locus_tag="QBH27_000865"
     CDS             complement(35223..36044)
                     /locus_tag="QBH27_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDQ7013219.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(36144..36227)
                     /locus_tag="QBH27_000866"
     CDS             complement(36144..36227)
                     /locus_tag="QBH27_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013220.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(36320..36655)
                     /locus_tag="QBH27_000867"
     CDS             complement(36320..36655)
                     /locus_tag="QBH27_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDQ7013221.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(37052..38305)
                     /locus_tag="QBH27_000868"
     CDS             complement(37052..38305)
                     /locus_tag="QBH27_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7013222.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNHFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTARYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            38412..39305
                     /locus_tag="QBH27_000869"
     CDS             38412..39305
                     /locus_tag="QBH27_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7013223.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            39440..40660
                     /gene="mlc"
                     /locus_tag="QBH27_000870"
                     /gene_synonym="dgsA"
     CDS             39440..40660
                     /gene="mlc"
                     /locus_tag="QBH27_000870"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDQ7013224.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            40785..41480
                     /gene="bioD"
                     /locus_tag="QBH27_000871"
     CDS             40785..41480
                     /gene="bioD"
                     /locus_tag="QBH27_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDQ7013225.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(41433..42725)
                     /gene="clcB"
                     /locus_tag="QBH27_000872"
     CDS             complement(41433..42725)
                     /gene="clcB"
                     /locus_tag="QBH27_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDQ7013226.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPTMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(42886..43500)
                     /gene="dmsD"
                     /locus_tag="QBH27_000873"
     CDS             complement(42886..43500)
                     /gene="dmsD"
                     /locus_tag="QBH27_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDQ7013227.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDNFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(43543..44397)
                     /locus_tag="QBH27_000874"
     CDS             complement(43543..44397)
                     /locus_tag="QBH27_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDQ7013228.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(44399..45016)
                     /gene="dmsB"
                     /locus_tag="QBH27_000875"
     CDS             complement(44399..45016)
                     /gene="dmsB"
                     /locus_tag="QBH27_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDQ7013229.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(45027..46596)
                     /locus_tag="QBH27_000876"
                     /pseudo
     CDS             complement(45027..46596)
                     /locus_tag="QBH27_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(46657..49083)
                     /locus_tag="QBH27_000877"
     CDS             complement(46657..49083)
                     /locus_tag="QBH27_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDQ7013230.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDKYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(49282..49587)
                     /locus_tag="QBH27_000878"
     CDS             complement(49282..49587)
                     /locus_tag="QBH27_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDQ7013231.1"
                     /translation="MKHSTYCAALLLALASPTVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            49695..50405
                     /locus_tag="QBH27_000879"
     CDS             49695..50405
                     /locus_tag="QBH27_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDQ7013232.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(50408..50968)
                     /gene="speG"
                     /locus_tag="QBH27_000880"
     CDS             complement(50408..50968)
                     /gene="speG"
                     /locus_tag="QBH27_000880"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDQ7013233.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(51003..51344)
                     /locus_tag="QBH27_000881"
     CDS             complement(51003..51344)
                     /locus_tag="QBH27_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDQ7013234.1"
                     /translation="MKITLSKRIGLLAFLLPCALVLSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            51479..51805
                     /locus_tag="QBH27_000882"
     CDS             51479..51805
                     /locus_tag="QBH27_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDQ7013235.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            52011..53225
                     /gene="rspA"
                     /locus_tag="QBH27_000883"
     CDS             52011..53225
                     /gene="rspA"
                     /locus_tag="QBH27_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDQ7013236.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            53237..54256
                     /locus_tag="QBH27_000884"
     CDS             53237..54256
                     /locus_tag="QBH27_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDQ7013237.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFDEKGIQPTLVIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            54314..55681
                     /gene="ydfJ"
                     /locus_tag="QBH27_000885"
     CDS             54314..55681
                     /gene="ydfJ"
                     /locus_tag="QBH27_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDQ7013238.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALQDAAEDQPHVVRAAQ
                     PSRSL"
BASE COUNT        14128 a        13479 c        14335 g        13770 t
ORIGIN      
        1 cacattcaat gcagtcggca atgttatgcg tggtggcttt atcgtgttgc tgacctttgc
       61 tgaaccagta cagctgttgc ggcaaaagat ccgcagggca ggcgtcagca caggcgctac
      121 aacggatgca actttgttct tcctgtggtt cgccaagttc attggcagag ggagccaaaa
      181 gacagttggt aatctttacg accgggacat ccagccacgg taaggtaaag cccattagcg
      241 ggccgcccat aatcaccatt tgatcggcag aggggcagaa tccggcatca ttcaataaat
      301 gacgcactgg cgtacccagc cgtgcccaga cgttgcccgg gcgagcgatt gcttcgccag
      361 tcagggtcac gacacgctcg gtaatcggct cgccatcaat aacggcacgt ttcactgcat
      421 aagcggtgcc gacgttttgc attaatacgc cgatatcgga tgaacgcccg ccatgtggaa
      481 cctgcttccc ggtcagaatg taggttaatt gtttagcacc gccagaagga tatttggtgg
      541 gaatcacccg cagagaaata tcgtgggagt ccgccagcac cgcgcgtagc atggaaatcg
      601 cctgcggttt gttatcttca atgccgataa gaatttcgcg cggctgcaga atatgcgcaa
      661 ggatgcggat accctctacg acctgagccg cgcaatcctg catcaaacgg tcatcggcgg
      721 taatgtacgg ctcgcactca gcagcgttga taatcaacgt ttcaatctta tctccgccgc
      781 cctgcaattt aacgcctgtc ggaaatcccg caccacccag cccggcaaca ccaaactgat
      841 gaatgcgctc gattaactct tcgcgactgc gagagcgata atcggcccag ccgtcgcgcg
      901 ggatccagca gtcttcgcca tcggcatcaa taatcacact taattcagct aaagctgaag
      961 gatgagccgt agagtggggc gcaatagccg taacggtacc cgaggtgggc gcgtgaaccg
     1021 gcagcatttt gccgcgacca cgggtaagcg gctggccgcg caatacttta tcgccgacgc
     1081 taacgcacaa ctcaccttca gcgccaatat gttgttttag tggaatgaca aaacgctgcg
     1141 ccagtggtac ctggcgcagg ggcgtaccgt tggactgggt tttcatctcc ggtggatgga
     1201 tgccgccgtt gaaatcccag attttatttt ttctgaatgc agagaataac ttaagcatgg
     1261 tgttccacgg gaatgatacg cacgggaatg gtgttcagat cccatttcca ggagtcagga
     1321 gtttctgcga ccggttgcaa cgagatgcag tgcgtcgggc acggatcaac acataaattg
     1381 cagcccgtac agagatcact cattaccgta tgcatggcgc gggtagcgcc aacgatggcg
     1441 tctaccggac acgcctgaat acatttagtg cagccaatac agttattttc atcaataacc
     1501 gccaccatcc gcgctggcgt tagctcttgc gcttcgccat ccagcggctg cggctcgaca
     1561 ttaagcaact cggcaatttt tagcatcaca gcttcgccac ctggggcgca acggttgatt
     1621 ttttcaccgt tacagctgat ggcttccgcg taggggcgac agccgggata accgcactga
     1681 ccacactggc tctgcggtaa gatttcgtca attttctcaa cgaccggatc gtcttccacc
     1741 gcaaaacggc gggaggcata acccagaatg gcgccaaacg ccagggccag caggctcacg
     1801 gcggcaacgg caatccagat agcattcatt acaacttcac caaaccacta aagcccataa
     1861 aggccagaga cataagacct gcggtaatta acgcaatggc attaccgcga aaaggtgccg
     1921 ggacatcagc cacagcaagg cgttcgcgga tggcggcgaa gagcaccatc accagcgaga
     1981 aaccgacagc ggcggaaaaa ccgtacagcg ctgactgcaa gaaattgtgc ccgagattga
     2041 tattcagcaa cgccacgccg agcacggcac agttggtggt gataagcggc aaaaaaatcc
     2101 ccagcagacg gtaaagtacc gggctggttt tgcgcaccac catttcggtg aactgcacaa
     2161 ccacagcaat caccagaata aacgccaggg tgcgcagata aatcagatta agtgggatca
     2221 aaatccacgt atcgataagc caggcgcaaa tagacgccag cgtcattaca aacgttgttg
     2281 ccagccccat gcccatcgcg gtttccagct ttttggaaac ccccataaac ggacagagac
     2341 cgagaaactt gaccagtaca aagttattga ccagtacagt tccgacaaac agtaacaggt
     2401 agtcagtcat ttttcggcct gaaataaaaa agccgcctat tatccgttaa tccagagcag
     2461 gcgacaacag gttaactgta aggttattac ggattcacaa acgtcgcttt cacccgcgac
     2521 gagcgtttga aataggggac gatcaatgcg gttgccaaca aagtaaacag caactggcga
     2581 acggctatgc catcttctac gggtgaaaaa gcgaacgctt ttactgccag caatacggaa
     2641 ataagcagcc agataatata gtgtttagga acacaacgcc gacgtttaaa gaaagcgatg
     2701 gtcaaccaca gtgtgtaata ccacatggcg atagcggtaa taaaagaaac gccccataag
     2761 atttgtgtcg tcagtgcttg tccgccgagc gtttgaaatg tttgcggaga agataacgca
     2821 gcggtataca gcaacaacgc cagcgtcgta ctcaacagag cgactaaaag ccaggccaac
     2881 gggccaagca accagcctcc aatacgttgt ggcgttgtgg tggtcatagg ttctccagaa
     2941 tagcgacgca aacagacaaa tctaatttta ggggcgttat tataaggctt ttataaccga
     3001 tcgctacttt ctttactgga catagtgcca gacggacttc ggtacctggc cgaggtcaaa
     3061 caaacgcccg ccagaaacca gctctgcgcg acggtgatcg gcagcacgat acatattgat
     3121 cagttcctga tcgtccgtca gggtgtaatt aagatggtcg tagagttttt ccagactttc
     3181 gagtgaactg attttgcgaa attttaataa gtagtcctga acagtcataa aaggtccata
     3241 aataattaat accaaagggg taggtattat tagtgatgat aaaatttcac caaaagtgaa
     3301 cgcttgtgtt attacaataa taagaatgcg ctgatggcaa atattcaggg atgaaaaact
     3361 gctctgaaaa gagtaacgcg ctttactatc cctgacaatc atcaacaaca tcgaacaaga
     3421 taataaattc ctggtttaat atccgacaag tgaaaacatg cacccggacg ggcagcatgt
     3481 cgctcaacaa gtgcagagct tacttgtgtt gtaccgtagt actctgttca ggtggctgat
     3541 agttgtcaat gtgactcgcc acgccaagaa gaatgactga aacgacaaga acgatccaac
     3601 ctgttaattc aataagacga ttcattacag cccacatctc tgttgattga tccattaact
     3661 tcagggggta aatgttactt agcaataata gctccagcaa gatttttact gaggtttttg
     3721 cgatatttag cttttgtcgt tggaaaaatc gctatttttt tgacttaagt taaacaacat
     3781 cccttattgc tggcaggtta ttaaactgtt gagcgagggt aaaggatagt gtcaaatagc
     3841 catcatactt caatgagagg caatgacatg agcgacaaca tccgtgttgg ggtgattggg
     3901 tatggttatg cgagcaaaac cttccatgcg cccctgattg cgggcacgcc cgggctggaa
     3961 ctggcggtaa tctccagcag cgatgaaaca aaagtaaaag ccgactggcc aacggttacg
     4021 gttgtctctg agccgaagca tctgtttaac gatcccaaca tagacctgat tgtcattcct
     4081 acacccaacg atacccattt tccgttagct aaagcggcgc ttgaggcggg taaacatgtg
     4141 gtcgttgata aaccctttac cgtgacactg tcacaagcac gagagctgga tgcgctggca
     4201 aaaaccctgg ggcgtgtgtt gtctgtattc cataaccgtc gctgggatag cgattttctg
     4261 accctaaaag gtttgctcgc ggaaggcgtg ctgggggaag ttgcttactt tgagtctcat
     4321 tttgaccgct tccgtccgca ggtacgcgat cgttggcgtg aacagggcgg cccaggcagc
     4381 ggtatctggt acgatttagc accgcacctt cttgatcagg ccattacgct atttggttta
     4441 ccggttagca tgacggtaga tttggcacag ttacggcccg gagcgcagtc gaccgattat
     4501 ttccacgcta tcttgtccta tccgcagcgg cgagtgattt tacacggcac catgctggca
     4561 gccgctgagt cagcacggta tatcgtgcat ggatcccgag gcagttatgt gaaatatggc
     4621 ctcgatccac aggaagaacg tctgaaaaat ggcgagcgtc tgccgcagga agactggggc
     4681 tacgatatgc gtgatggcgt acttacccgc gtggaaggtg aggaacgtgt cgaagaaacg
     4741 ctgttgacag tgccggggaa ttatccggct tactatgcgg ctattcgtga tgcgttaaat
     4801 ggcgatggtg aaaatccggt tccggcaagt caggcaatcc aggtaatgga gttgattgag
     4861 ctgggcatcg aatccgccaa acatcgcgcg actttgtgcc ttgcgtgaaa aggggatggt
     4921 gcaattagca ccatccattt ttgttacttc gcggcgactt tttctcgcag tgcgcgtttt
     4981 tcctctgcgc tgaggaaagc catttccaga ccattaatct gtgcctggcg gatttgctcg
     5041 cgggataacc cagcagctgg cgcggcaacg gtatattcgt gaatgatatc cactccctgc
     5101 acgccgggat cgtcagtgtt aatgctggca cgaatgccat gctcaaggaa cgttttcagc
     5161 ggatgtgcag ccagatccgc cacggtgctg gtctgaatat tggaggtcag acaggattca
     5221 ataccaattt gttgctcggc gagaaaatcc atcagcgccc gatcttcaat ggcttttacg
     5281 ccatgtccaa tacgctccgc ccccagttca cgaatcgcct gccagatgct ttccggtccg
     5341 gcagcttcgc ctgcatggac ggtaatatgc cagcccgcat cacgcgcgcg gttgaaatga
     5401 gaaaggaaca gacttcccgg gaaaccaagt tcatcaccgg ctaaatcaag tgcggtaatc
     5461 tggtcacggt gggctaaaaa ggcctccagc tcttgctgac aggcggcttc gccgaaggtc
     5521 cggctcataa taccgataag cttcgcctgc acaccaaagg tgcggcaacc ttcacgtacg
     5581 ccatcgatca ccgcttcgac aacacccgct acaggcagct gatgtgccat tgccatgtag
     5641 cctggtgaaa aacgcagttc gacatagtgc aggccgttac gggctgcatc ttcaatgttt
     5701 tcaaatgcca cgcggcggca ggcatcaaga gaggcgagaa ctttaacgcc ccagtcaagt
     5761 ttagtcagaa agctcaccag atcgggttcg ttggcaatga cctgaacgtg gggagtcagt
     5821 gtttccaggg attgtgcagg aagcgagata ttatactggc ggccaagttc aagaatggtc
     5881 tggggacgaa tgttgccatc aaggtggcga tggatatcag ttaatggcag ggtggtatca
     5941 atcatggtcg cactcttttt ctggatataa aagtgcgcac cattataaaa acaaagcagg
     6001 gtaaaaatcc acgattgcgc aacgattttt tacgttgcgc aattggggtt aaccaacagc
     6061 gcggatggcg ttaattaatc cagccacacc tttttccagt ttcgaacgtg ggcagccggc
     6121 attgagtcgg acaaaaccac gaccttcttc accgtaggta tatcccggca tgatcgcgat
     6181 cttttcttgc tcgataagcg ctttttgcaa cgcgttgtcg tcaatattca acggacgtag
     6241 atcaagccag gccagataag tggattgcgg gatctgccag ttgagttcag gaaacgcagc
     6301 gttcatttta tctgcgatat acgtcaggtt atctttcaga tagacgcgta aggcatccag
     6361 ccatggcgcg ccttgctgat aggcggcgat atgagcagtt aacgccagta ccgaagggga
     6421 agaaagccca tcacgtcctt tcagtgccga taaataggca tcgcggctac tgctattttc
     6481 tataatcccg taagcaccgg tcagggcggg aatattgaaa ctttttgatc ccgacgttag
     6541 caacgcccag tctccgcgcg ccacattact ccagggaata tgcggctgct cgccccaaac
     6601 catatccata tggatttcat cggaaataac ccgcacacca tgacgctcgc acaggtcagc
     6661 catgatctcc agctcatcgc acgtccacac tttcccggta ggattctgtg ggctacacag
     6721 gagcataatt ttacattctg gtttcgccag cacggcttcc agcttgccca tatcgcaaaa
     6781 ccagccgtca gcctgcttct ctaaagcaac gggcattact gtgcgctggt taccttcaat
     6841 ggccttgtaa aatgcgtcat aggcgggtgt gtggatcacc acgccttcac ctgtttcaga
     6901 ccactgacga atcagttctg aaaccatata gataacagaa gggccataca ccaccgactg
     6961 aggatcgatg gcggtgtaat gctgggtgga aaaccagtgg gcaatagccg cgagaaactc
     7021 atcgtttttc cagcggctgt agccaaacac gccgtgtatc aggcgctgat tcagcgcctc
     7081 gataatgcag ggggcagtgg caaaatccat gtctgaaatc gtgaacggta acaggtcagc
     7141 agtgccgaaa cggtcagcga cataatccca ctgcgtacac catgtgccat gacgatccac
     7201 gacctttgaa aaatcgaaca tatcttatcc ttatgcctgg acagtacgca tcagaccgac
     7261 catttcatct tttactgact gaacttgtgg cccgataaca acctgcaggt tatgttgatt
     7321 aagttgtact acgccaattg cccgattgtc cttcagtgcc tgcacattaa caagcgacat
     7381 atcttttaca gacaaacgca gacgcgtaat gcagttatcg agactgacaa tattgtcggc
     7441 accgcctaag gcttcgagga ttgccggaac gttgtaacct gatttacccg gcgcaccggc
     7501 aacggctttt tcgattgaac tggcaacttc actatcgcgc cccggggttt tgagattgaa
     7561 gcgggtgata gcgaaacgga agatgatgta gtaaacgaca aaccagattg ccgccactac
     7621 tggcaccatg taccacttgg ttgacagacc atgcaaaata ccgaacacca caaagtcgat
     7681 gatattgccg tcggtattac cgatggtgac gccgagcaca gacatgacag tgaagccgag
     7741 gccggttaac agcgcgtgga tgacatacag aactggcgct acgaacagga acaggaattc
     7801 cagaggttcg gtcgtgccgc caacgacaca ggcgatcagg ccagaaatca gcagaccttt
     7861 aattttatgg cgattttccg ggcgcgcaca gtgatacata gctaacgctg cacctggcag
     7921 accgccgaga aacgcaggca ttttaccttg cgaaaggaaa cgcgtggcgc tttcagaaaa
     7981 accgtgagtg gtcgggcaac tcaattgcgc ctggaagatg gtcagtgcac cgctgacggt
     8041 ttgaccgcag acttcctgcg tgccgcctgc gtcggtaaag cgaattaatg ccaccagaat
     8101 gtgatgcaga ccaaacggca acagcagacg ttcaccggta ccaaacagca tcggtccgaa
     8161 atcacccgcg ctgttgatca tatggcctaa gccgctaata cccatggcga aaatcggcca
     8221 gactaatgga atcaccaggc cgacaagacc catcaccagc gaggagatga ttggtacgaa
     8281 gcgcgtaccg ccgaagaatg ccagcgcatc cggcaggcgg atattatgga atcgctcatg
     8341 cagcatccag acgataatac cggcgatcac cgcaccgagg atcccggtgt cgatcgactg
     8401 gatcccaaga atgctctgga tgttattggc tttcagaacg gcggcgtcca tggttggcag
     8461 aatgccttta ttggtcaacc agaagtttac cgcgaggttc attaccgcat aaccgacgaa
     8521 gccagcgaat gccgctacgc ctttgttttc gcgtgccaga cccagcggga tggcgataca
     8581 gaacatgact ggcaggaaac taaaagcaaa cgagccaatc ttactcatcc aggtaaagat
     8641 agcctgcaac acggggttgc ccaggaccgg gatcagggtg atgacatcat ggctgctaag
     8701 agaactacca atgccgagca taatgccgca gaacgacaat aatgccacgg gtaacatgaa
     8761 ggttttgcct aactgctgga agaactccca cagcgtgact ttcggtgctg ttttcgccgt
     8821 cataaaacga ctcctcgtag agaacaatct ggattcagta aattgcgcga gaagataaaa
     8881 cgttttatca aattttagtg aggcataaat cacattacgc aacgataata gcgggtataa
     8941 gataaataaa aggtaaaacg ttttatctgt cacataatca gggagtaggt catctgcatg
     9001 gctaccgcca aaaaaataac cattcatgat gttgcgctgg ctgcgggcgt gtcggtaagt
     9061 accgtttcgc tggtgcttag tggcaaaggg cgaatctcta ccgccacagg agaacgcgtt
     9121 aacgccgcca ttgaagagct gggatttgtg cgcaatcggc aggcgtcggc gctgcgcggc
     9181 gggcaaagtg gcgtcattgg tttgatcgtc cgtgatttat ctgcgccgtt ttacgccgaa
     9241 ttgacggccg gattgacgga agctctggaa gcgcagggac ggatggtttt tttgcttcac
     9301 ggcggtaaag acggcgagca gctggcacag cggttttcac tattactgaa tcagggtgtc
     9361 gatggtgtgg taattgccgg ggctgcagga agcagcgatg acctgcgacg gatagcagaa
     9421 gaaaaagcta tcccggtgat tttcgcttcc cgtgccagtt atcttgatga cgttgatacg
     9481 gttcgcccgg acaacatgca ggctgcacag ttgttgacgg agcatctcat tcgcaatggg
     9541 catcagcgta tcgcctggct gggagggcaa agttcctcat taacccgggc agaacgggtg
     9601 gggggctatt gtgcgactct actaaaattt ggcctgccgt ttcacagcga ttgggtgctg
     9661 gagtgcactt ccagccagaa gcaagccgcg gaagctatca cggcgctttt acgtcataac
     9721 ccgaccatca gcgccgtggt ttgctataac gaaactattg cgatgggggc atggtttggt
     9781 ttgctgaaag caggaaggca aagcggggaa agcggagtcg atcgttactt tgagcaacag
     9841 gtttcgctgg cggcatttac cgatgcaacg cctaccacac ttgatgatat acccgttatc
     9901 tgggccagca cgccagcgcg ggaacttggt accacacttg cggatcgcat gatgcaaaaa
     9961 atcacccatg aagagacgca ttcacgcaat cttattattc ccgcccggct cattgcggcg
    10021 aaataatcct ctctttatct gctatacctg gtagtgtcct ttcctcaagg ttaatgataa
    10081 aaaatgtggc acacaggcgg tgccttttat ttttactgaa gttcacagga ggtttatgtg
    10141 tttaattctg acaacctgag actcgacgga aaatgcgcca tcattacagg tgcgggtgca
    10201 ggtattggta aagaaatcgc cattacattc gcgacagctg gcgcatctgt ggtggtcagt
    10261 gatattaacg ccgatgcagc taatcatgtt gtagatgaaa ttcaacaact gggtggtcag
    10321 gcatttgcct gccgctgcga tattacttcc gaacaggaac tctctgcact ggcagacttt
    10381 gccgtcagta agttgggtaa agttgatatc ctggttaaca acgccggtgg cggtgggcct
    10441 aaaccgtttg atatgccaat ggcagatttt cgccgcgctt atgaactgaa tgtattttct
    10501 tttttccatc tgtcacaact tattgcgcca gaaatggaaa aaaatggcgg tggcgttatt
    10561 ctgaccatta cttctatggc ggcagaaaat aaaaatataa acatgacctc ctatgcatca
    10621 tctaaagctg cggccagtca tctggtcaga aatatggcgt ttgacctggg tgaaaaaaat
    10681 attcgggtga atggcattgc gccgggggca atattaacca atgccctgaa atccgttatt
    10741 accccagaaa ttgaacagaa aatgttgcaa cacacaccaa tcagacgtct gggccaaccg
    10801 caagatattg ctaacgcggc gctgttcctt tgttcgcctg cagccagctg ggtaagcgga
    10861 caaattctca ccgtctccgg tgatggggta caggaactca attaatacac taacgaaccg
    10921 gtaaacagcc gtatgtgttg tttaccggga tagcgtgtgc aactcgtcaa cgtctctgct
    10981 aaacaactgg cagccaaatc acggctattg gttaaccaat atctggataa aaagcttacg
    11041 aattgagtgt gccttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt
    11101 aaactagtta ttcatgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca
    11161 caaccgacac ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt
    11221 cacagtgcct cgatgaaagc catctgtaaa tcctgcgcca ttagtcccgg gacgctctat
    11281 caccatttca tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg
    11341 gcgctggccc gtttccggga accgattgaa gggattcatt tcgttgacta tatggtcgag
    11401 tctattgtct ctctcaccca tgaagccttt gggcaacgag cgctggtggt cgaaattatg
    11461 gcggaaggga tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc
    11521 acggaatttg ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac
    11581 atcaacacgg caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat
    11641 atcgaagcgg aagatctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt
    11701 ggcggtatat taaccgcatc ctgatcctct ctctctttcc gcgggctggt gacaactgag
    11761 cccgcgtttc atatcgtaat ttctctgtgc aaaaatcatc ctcccaggga tcggagaatt
    11821 ccacctaaat attcatcgta gcaatatatc atgagggttt atcgttccca atatgttcga
    11881 atgaacgttc gattgcttat tttgtggctt ctgtcaacgc tgttttaaag attaatgcga
    11941 tcaatatcac gctgtgggta ttgcagtttt tggttttttg atcgcggtgt cagttctttt
    12001 tatttccatt tctcttccat gggtttctca cagataactg tgtgcaacac ggaattggtt
    12061 aactaatcag attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac
    12121 gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc
    12181 tggatcgcga aaactgtgga attgatcagc attggtggga aagcgcgtta caagaaagcc
    12241 gggcaattgc tgtgccgggc agttttaacg atcagttcgc cgatgcagat attcgtaatt
    12301 atgtgggcaa cgtctggtat cagcgcgaag tttttatacc gaaaggttgg gcgggccagc
    12361 gtattgtact gcgtttcgat gcggtcactc attacggcaa agtgtgggta aataatcagg
    12421 aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcaca ccgtatgtta
    12481 ttgccgggaa aagtgtacgt atcactgttt gtgtgaacaa cgaactgaac tggcagacta
    12541 tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct tactttcatg
    12601 atttctttaa ctacgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct
    12661 gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcatctgttg
    12721 actggcaggt ggtggcaaat ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg
    12781 tggttgcaac tggacaaggc accagcggga ctttgcaagt agtgaatccg cacctctggc
    12841 aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg
    12901 atatctacct gctgcgcgtc ggtatccggt cagtggcagt gaagggcgaa cagttcctga
    12961 tcaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg gatttgcgcg
    13021 gcaaaggatt cgataacgtg ctgatggtgc acgatcacgc actaatggac tggattgggg
    13081 ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg
    13141 aacatggaat cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc tctttaggca
    13201 ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca gtcaacgggg
    13261 aaacccagca ggcgcactta caggcgatta aagagctgat tgcgcgtgac aaaaaccacc
    13321 caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg
    13381 aatattttgc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct
    13441 gtgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc
    13501 tgtgcctgaa ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga
    13561 aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca
    13621 ccgaatacgg tgtggatacg ttagccggcc tgcactcaat gtacaccgac atgtggagtg
    13681 aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgctg
    13741 tcgtcggtga gcaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg
    13801 ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc
    13861 tgctgcaaaa acgctggacc ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac
    13921 aatgaatcaa caactctcct ggcgcgccat cgtcggctac agcctcggtg acgtcgccaa
    13981 taacttcgcc ttcgcaatgg gggcgctctt cctgttgagt tactacaccg acgtcgctgg
    14041 cgtcggtgcc gctgcggcgg gcaccatgct gttactggtg cgggtattcg atgccttcgc
    14101 cgatgtcttt gccggacgag tggtggacag tgtgaatacc cgatggggaa aattccgccc
    14161 gtttttactc ttcggtactg cgccgttaat gatcttcagc gtgctggtat tctgggtgcc
    14221 gaccgactgg agccatagca gcaaagttgt gtatgcatat ttgacctaca tgggactcgg
    14281 gctttgctac agcctggtga atattcctta tggttcactt gctaccgcga tgacccaaca
    14341 accacaatcc cgcgcccgtc tgggcgcggc tcgcgggatt gcgcttcatt gacctttgtc
    14401 tgcctggcat ttctgatagg accgagcatt aagaattcca gcccggaaga gatggtgtcg
    14461 gtatatcatt tctggacaat tgtgctggcg attgccggaa tggtgcttta cttcatctgc
    14521 ttcaaatcga cgcgtgagaa tgtggtacgt atcgtggcgc agccgtcatt gaagatcagt
    14581 ctgcaaaccc tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc gctgtgtgtg
    14641 ctgatttcga ccttcgcggt cagcgcctcg tcgttgttct acgtgcgcta tgtgttaaat
    14701 gataccgggc tgttcactgt gctggtactg gtgcaaaacc tggtcggtac tgtggcatcg
    14761 gcaccgctgg tgccggggat ggtcgcgagg atcggtaaaa agaatacctt cctgattggc
    14821 gctttgctgg gaacctgcgg ttatctgctg ttcttctggg tttccgtctg gtcactgccg
    14881 gtggcgttgg ttgcgttggc catcgcatca attggccagg gcgttaccat gaccgtgatg
    14941 tgggcactgg aagctgatac cgtagaatac ggtgaatacc tgaccggcgt gcgaattgaa
    15001 gggctcacct attcactatt ctcatttacc cgtaaatgcg gtcaggcaat cggaggttca
    15061 attcctgcct ttattttggg attaagcgga tatatcgcca atcaggcgca aacgccggaa
    15121 gttattacgg gcatccgcac atcaattgcc ttagtacctt gcggatttat gctactggca
    15181 ttcgtcatta tctggtttta tccgctcacg gataaaaaat tcaaagaaat cgttgttgaa
    15241 attgataatc gtaaaaaagt gcagcagcaa ttaatcaacg atatcactag ttaatattca
    15301 ataaaaataa tcagaacatc aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta
    15361 tttgcctgac ggctgcctct ggccttacct ctgcttacgc ggcacaactg gctgacgatg
    15421 aagcgggact acgcatcaga ctgaaaaacg aattacgcag ggctgataag cccagtgctg
    15481 gcacgggaag agatatttac gcatgggtac agggaggatt gctcgatttc aatagtggtt
    15541 attattccaa taattattgg cgttgaaggc ggggcgtatt atgtttataa attaggtgct
    15601 cgtgctgata tgagtacccg gtggtatctt gatggtgata aaagttttgg ctttgccctg
    15661 ggggcagtaa aaataaaacc cagtgaaaat agcctgctaa aattaggtcg cttcgggacg
    15721 gattatagtt atggtagctt accttatcgt attccgttaa tggctggcag ttcgcaacgt
    15781 acattaccga ctgtttctga aggagcatta ggttattggg ctttaacgcc aaatattgat
    15841 ctgtggggaa tgtggcgttc acgagtattt ttatggaccg attcaacaac cggtattcgt
    15901 gatgaaggtg tttataacag ccagacggga aaatacgata aacatcgcgc acgttctttt
    15961 ttagccgcca gttggcatga tgataccagt cgctattctc tgggggcatc ggtacagaaa
    16021 gatgtttcca atcagataca aagtattctc gagaaaagca taccgcttga cccgaattat
    16081 acgttgaaag gggagttgct cggcttttac gcgcaacttg aaggtttaag tcgtaatacc
    16141 agccagccca atgaaacggc gttggttagt ggacaattga cctggaatgc gccgtgggga
    16201 agcgtatttg gcagtggtgg ttatttgcgc catgcaatga atggtgccgt ggtggatacc
    16261 gacattggct atcccttttc attaagtctt gatcgtaacc gtgaaggaat gcagtcctgg
    16321 caattgggcg tcaactatcg tttaactccg caatttacgc tgacatttgc accgattgtg
    16381 acccgcggct atgaatccag taaacgagat gtgcggattg aaggagcggg tatcttaggg
    16441 ggtatgaact atcgggtcag cgaagggccg ttacaaggga tgaatttctt tcttgctgcc
    16501 gataaaggac gggaaaagcg cgatggcagt acgctgggcg atcgcctgaa ttactgggat
    16561 gtgaaaatga gtattcagta tgactttatg ctgaagtaag aaaaaacgcc ggagagaaaa
    16621 atctccggcg ttttggctgt gaattactgc tgcggaatag cgggtacagc cggaacgttt
    16681 aatgtcggca tcgcaaacat acccacgaaa tcttccagtg gcattttctg cccgtttaac
    16741 gttatctgac cgttagcata ttgcaggctg gtggtgatgg tattgtcctg caaggtggtc
    16801 agacggaaca tctgccccat tgctgatgca ccttcaactt gctgtttcgc cagttttttc
    16861 gcttgatctt cctgataacc ttccagcttc gctacctgag tcatcaactc agttgccata
    16921 tccaccggaa tggtcagttt cgcatccaga gatttaaccg agcggtccac ttcctgcgcc
    16981 agcgtttgcg gcgcttcttt agtcgttgcc ggatctttca ggaacagcga cagattcagc
    17041 gcactttcac cctgactgtt tttccagctt agcggcgcga tagtaatcac cggatcgcct
    17101 ttcagcatca gcggcagagc gctaaagaag gcttccgtca ctttctcctg ataaagttcg
    17161 ggattgttgg caatttctgg ctgtgccagc agcgcctgag tttgcgcgtt atattgctgg
    17221 ctaaactgat gccaggcttc gccatcaatt tggccgactt ttaaagtcag cttgccactg
    17281 cccagatcct gattctgtac cttcaggctg tttagcgagt aatccagttg gctattgatc
    17341 gttttaccgt cattgaccag atccgattta ccgctgatct ccatgccttc cagcagtgcc
    17401 agttctttgc cctccactga aatggtcatt ttttccagcg acagtttttg gtttcctacg
    17461 cgctcaccaa aacttgccag cgtgctggaa ccgtcggttt tcagattatt aaaggttaac
    17521 tgcactttct ggttgtattc gttaaccgcg tctatccgac cactttgcgc ctccccggaa
    17581 agggagatag ctttgccgtc tctgtctgca tttaactgga actcgccgcc gctaaaggcg
    17641 actttttcat ccttttgctc gtaattcagt ggcttgagcg aaatgtcgga actggaatct
    17701 ccgctgtaac caatgcggga gttaatctca aaaggcgttt caccttttgc catatcaaac
    17761 agtggtttgc ttacttcgtt attaaccagc gtggtttgaa ttgatgccat cgacgggatc
    17821 agattcagtt ttttaagctg ggcaagcggg aagggaccat gatcaaccga ttcgttgaag
    17881 atgacgctct gaccgctttt aatccacaga tttacttttc cggcaatggg tttcaccaac
    17941 agttgcaact ggctgctgaa tacgccgcga tgatagtttt gataactgac ttccaggttg
    18001 gattcaggag ctgtcagttt gagttgcgcg ttcgcctgcg cgaccatgtc ttcgagatgg
    18061 gtttcaatct tcttgcctgt ataccatgcg ccgcctgtcc agactacgcc tagcgcaaca
    18121 atgacgccta ccgctaccag cgatttattc ataatgatta tccataaaat gaaatcaggc
    18181 ggactggccg cctgaaggtg ttataagcct ttaataagct tagcaagaga tgttaatttt
    18241 ttcagtaagc tcttacagct tgttgtaaac acgcgctaaa cggccgtggc ctttgacagt
    18301 caccggtgat tcgttggcgg caataaacgc tgattcaccc ggtttaagct gtaactgctg
    18361 agaaccttta cacagcgttg catcgccttc gacgcagaac aaaatggcgg cactctgctg
    18421 gctgatggtg gtttctttat cactaaggtc atgcaacgag aaggcaaaat catccacagg
    18481 aatcgggaag tccagttctg caccttgttt caccggctgg gtcaacaact tgttagccgg
    18541 tttggcttcg aacttcacgt tggcaaccag ttccggaata tcaatgtatt taggcgtcag
    18601 acctgcacgc agcacgttat cggagttcgc catcacttcc agcgccacgc cttgcaggta
    18661 agcgtgcggt gtttcagcga acaggaacat cgcttcgcca ggattcaatt tcaccacatt
    18721 cagcaacagc ggggagaaca ggccgctgtc ttccgggtaa aattcagaaa ttaaacgaat
    18781 cgtttgccat ggttcaccct gctggctgtc gagggccgat tttaaaatcg ccagcgcgcg
    18841 ggatttttct tcaccctgca tattcaacag gctggcgaac agttcgctta aacgttcggc
    18901 atcaggctgt tgtaaaaagt gagcaatcgc cggatgtgca cctgcgaccg gctggagcag
    18961 ggagacaatc tcggaaaact cacgaaacgc gttcatcgcg aggaaagggg tcagggcgaa
    19021 aaccagctca ggcttgtgat taggatcttt atagttacgc tcagcggcat ccatcggaat
    19081 acctgcagca ttttctttgg caaaaccgat ttcagaattg cgtttgtttg gatgaacctg
    19141 aatggagagc ggctgggctg cgcataatac tttgaacagg aaaggcaact cgccaaagcg
    19201 tttggcaacg gcatctccca aaagagtcgc tttatcactc tcaatcacat cacgcagtga
    19261 aacgatatct cctgcggcat cgtgcacacg tgaactgctc tttggatgcg cgcccatcca
    19321 caactcggcc atcggctggc tggacggatt ttccatacca taaagttcag tcaacgccgt
    19381 tttgctgccc caggcatagt tttgcactga gttaatgagt ttttgcatga tcaatccctg
    19441 ttttaatgtg gaaattaatc ccgctattaa agcaagaatc ctacgggaag taacctggag
    19501 ccgcaaaaag tcgtactagt ctcagttttt gttaaaaaag tgtgtaggat attgttactc
    19561 gcttttaaca gggcaacgga acacccgccc agagcataac caaaccaggc agtaagtgag
    19621 agaacaatgt caaacaaacc ctttcattat caggctcctt ttccactcaa aaaagatgat
    19681 actgagtatt acctgctaac cagcgaacac gttagcgtat ctgaatttga agggcaggag
    19741 attttgaaag tcgcacccga agcgttaact ctgttggcgc gtcaggcgtt tcatgatgcg
    19801 tcatttatgc tgcgtccggc tcaccaacaa caggtggccg acattctgtg tgacccggag
    19861 gccagcgaaa atgataaata tgtggcgctg caattcctgc gtaactccga catcgcggca
    19921 aaaggcgttc tgccaacctg tcaggatacc ggcaccgcga ttattgttgg taaaaaaggg
    19981 cagcgtgtat ggaccggtgg cggtgatgaa gcagcgctgg cgcgcggtgt ttataacact
    20041 tatatcgagg ataatctgcg ctactcgcaa aacgcgccgc tggatatgta taaagaggtg
    20101 aataccggca ccaacttgcc agcgcagatc gatctttatg ccgttgatgg cgacgagtac
    20161 aaattcctct gtatcgccaa aggtggcggt tcggcaaaca agacgtatct ctatcaggaa
    20221 accaaagcgt tactgacgcc gggaaaactg aaaaattacc tggttgagaa gatgcgcacg
    20281 ctgggtacgg cggcctgtcc tccgtatcat attgcgttcg ttattggtgg aacttctgca
    20341 gaaacgaatc ttaaaacggt gaaactggct tccgctaaat actatgatga actgccaacg
    20401 gaagggaatg agcacggtca ggcgttccgc gatgtggaac tggaaaaaga attgctgatc
    20461 gaagcgcaaa atcttggtct gggtgcgcag tttggtggta aatacttcgc tcatgacatc
    20521 cgcgtgattc gcctgccacg tcacggcgca tcctgcccgg tcggtatggg cgtctcctgt
    20581 tctgctgacc gtaatatcaa agcgaagatc aaccgtcagg ggatctggat cgaaaaactg
    20641 gaacataatc caggcaaata tatcccggaa gagctgcgca aagcaggaga aggcgaagcg
    20701 gtgcgcgttg acctcaaccg tccgatgaaa gagatcctcg cacagttgtc gcagtatccc
    20761 gtttctacac gcttatcgct taacggcacg attatcgtcg gtcgtgatat tgctcacgcc
    20821 aaactgaaag agcggatgga taacggtgaa gggctgccgc agtatattaa agatcatccg
    20881 atttactacg cgggtccggc caaaacgccg gaaggttatg cctccggttc tcttggccca
    20941 acgaccgccg gacggatgga ttcttatgtc gatcaactgc aagcgcaggg cggaagtatg
    21001 atcatgctgg cgaaaggcaa ccgcagccag caggtgacgg atgcctgtaa aaaacacggc
    21061 ggcttctacc tcggcagtat cggtggtccg gcagctgtat tggcgcaggg aagtattaag
    21121 agcctggaat gtgttgaata tccggaactg ggaatggaag ccatctggaa aattgaagtg
    21181 gaagatttcc cggcgtttat ccttgtggat gataaaggaa atgacttctt ccagcagata
    21241 caactcacac aatgcacccg ttgtgtgaaa taaacagagc cgcccttcgg ggcggttttt
    21301 ttacatggca cgaaagacca aacgtttgtt atcaaatggt aaataatgag tgagctaaaa
    21361 gttgcttaac gaaagcaaaa cagaaagaaa aaattaatca ggtgaggagc aggccatgaa
    21421 tacagtacgc agcgaaaaag attcgatggg agcgattgat gtcccggcag ataagctgtg
    21481 gggcgcacaa actcagcgct cgctggagca tttccgcatt tcgacggaga aaatgcccac
    21541 ctcactgatt catgcgctgg cgctaaccaa gcgcgcagcg gcaaaagtta atgaagattt
    21601 aggcttgttg tctgaagaga aagcgagcgc cattcggcag gcggcggatg aagtactggc
    21661 aggacagcat gacgacgaat tcccgctggc tatctggcag accggctccg gcacgcaaag
    21721 taacatgaat atgaacgaag tgctggctaa ccgggccagt gaattactcg gtggcgtgcg
    21781 cgggatggaa cgtaaagttc accctaacga cgacgtgaac aaaagccaaa gttccaacga
    21841 tgtctttccg acggcgatgc acgttgcggc actactggcg ctgcgcaagc aactcattcc
    21901 acaacttaaa accctgaccc agacgctgag tgaaaaatcc cgcgcatttg ctgatatcgt
    21961 caaaatcggt cgtacccact tgcaggacgc cacgccgtta acgctggggc aggagatttc
    22021 cggctgggta gcgatgctgg agcataatct caaacatatc gaatacagcc tgcctcacgt
    22081 agcggaactg gctctgggcg gtacagcggt gggtactgga ctaaataccc atccggaata
    22141 tgcgcgtcgc gtagcagatg aactggcagt cattacctgt gcaccgtttg ttaccgcacc
    22201 gaacaaattt gaagcgctgg cgacctgtga tgccctggtt caggcgcacg gcgcgttgaa
    22261 agggttggct gcgtcactga tgaaaattgc caatgatgtc cgctggctgg cctctggccc
    22321 gcgctgcgga attggtgaaa tctcaatccc ggaaaatgag ccgggcagct caatcatgcc
    22381 agggaaagtg aacccaacac agtgtgaagc attaaccatg ctctgctgtc aggtgatggg
    22441 gaatgacgtg gcgatcaaca tgggtggcgc ttccggtaac tttgaactga acgtcttccg
    22501 tccgatggtg atccataatt tcctgcaatc ggtgcgcttg ctggcagatg gcatggaaag
    22561 tttcaacaaa cactgcgcag tgggcattga accgaatcgt gagcgaatca atcaattact
    22621 caatgaatcg ctgatgctgg tgactgcgct taacacccac attggttatg acaaagccgc
    22681 cgagatcgcc aaaaaggcgc ataaagaagg gctgacctta aaagcggcgg cccttgcgct
    22741 ggggtatctt agcgaagccg agtttgaccg ctgggtacgg ccagaacaga tggtcggcag
    22801 tatgaaagcc gggggttaat ctgcaacata caggtgcagc cgtggaatga ttaaacgaag
    22861 cggctgcgcc tgtggtttat aacggtgctg cacattgtcg gcatcgtaat ttaacaactc
    22921 accaacgtcc ggcacgcccg cgccattatc ccgattaatc agcgcaatca gcggcgtagg
    22981 gcaggcgtgt tgtacttgtt tttgatctcc cttataccag acgcgggcaa tcggctgcac
    23041 cttcaccgga cgtttgattt ttaacttcgc gttctgtggc agggcagcga tatcctgata
    23101 ttctcgctcc agttttcttt gccactcctc gcgcgtccac ggtgcgacac tgcgtggtga
    23161 tttcaggctt ttttccagct gtgccaggac ttcatcacga tgcaaatttt taatgatatg
    23221 tttattagcc cagccaaaac gtaaagtggc ggggtcgtgc agaacggtga gcgtgcggta
    23281 agcattaagg gtaatcagcc ccggaaaatg acgatgtacc cattcaaaac gtgccacgga
    23341 ggggagttct gattcaaccg tgacgatatg ctcgaacgtg gtcttgagtt tattgatgtg
    23401 ctgaatatga ctgaccaacg ctgcttgcga aaagttatcg acctggtaac acaacacgcc
    23461 aggcagacgg acggcggctt tgctgctgcg attttcggac tgttgttgaa taaataaatg
    23521 gcggaaatga cgcaacgcca gcgactgcgc gtcgttgccg agatgttgtt ttacctcaat
    23581 acgattaagc ggattatgct catcctcttt ttttacctcc ggcaaagaga acacgcgggc
    23641 aaccaatagc ttgtgttgct caagatgagc ggcaaatgca gccagctctt gttccatctg
    23701 gcgaaaagta gtgttgagtc ggtctacgag atcgtaacgc gccataatat taatcacttt
    23761 agttacaaca tccttatttt atagcacagt ggtggtgact tgaccaccgt gcgcatagtg
    23821 tcaggcagag gtaaattgcg ggatgttatg ccataacggc cagctgaacg agaagcgggc
    23881 accccccagt tcgctggtgt cacaattaac cgtaccgccc attgccagtg ctatagagtg
    23941 gacaatggcc agccccagcc cgcagccgcc ggttgagcga tcccggctgg gatcgaggcg
    24001 aacaaaaggt tcaaagatat gttcgcggtt ttctggggca atccctgggc catcatcctc
    24061 aacaattaat gtcgctctat tccccgacag tagcaggctg gtttcaaccg ttgagtgaca
    24121 gtagcgcagg gcgttattga gcaaattatc cagcacgcgc tccattaagc gcatatccaa
    24181 cgcagcataa tggccttgcg cgagcgtttt tatccgtacc gttttatcgg gcgttactgc
    24241 ctgaatatct gccagatgcg ttgacagcca caacggcagg tctggttcgc taagatgaag
    24301 ctcattttgt gggcgatcga gccgggcata agtcagcagc tcttcaatta aagcttcaag
    24361 ttgactgata tcgcgattca acgcctggga ttcggcggcg ctcaggttat cgctcatctc
    24421 cagtcgataa cgcaggcgca ctaacggtgt tcgcagttcg tgagcgatac cgtcaataag
    24481 ctgttttttg ctggcaatca aggcgttgat attgtccgcc atctggttaa atgcgacgcc
    24541 aagtcgttca aagctcgaac cttcatcaaa gtggatacgc tcactgagat gcccatcgcc
    24601 aaatcgttgc gccgctgctt ccagttttaa catatcctgc cagtgcggac gcatccagat
    24661 aaacaccgga aaggcgaggg aaatggcaat aaaagcgatc agggcgatat ccagcaatcg
    24721 catctgatgg aggtaataaa gataaggaac aggaccaact gccagcacgt agtggctgcg
    24781 tgggatacgt tgcaaaaaag tgtactgatc gtccagagcg acaatttcgc cgccacgcag
    24841 ccggtgcatg gaaatatcat caagatgata tttactcagc ggctcgacac gcagatcgaa
    24901 agagagattt aaatccatct ctttcagcgt cttaccccag tcgtgtgggg ggatctcacg
    24961 caattcgctg cgcatcagat acagcgaact gttcatcaaa tcatccagcg actgtttgcc
    25021 tgcgcgttcg gcggtaaatt tgtacaccag cccgaccagc agagacatca caaggaagca
    25081 gacaaacaat aacaggtaaa actggataaa cagttttttc atcgcttatt cccacgcatg
    25141 aggcgcaaaa agatagcctt tgttacgcac ggttttaata cgataaggtt ctgcggcgtt
    25201 atcgagcagt ttttttctta accgcgaaat agccacgtcc acgctacgat ccagcccgtc
    25261 ataactgacg ccgcgtaaat ttttcagcaa tgcatcgcgg tccatgattt gcccggcatg
    25321 ggtagctaat tcccacaata attcgaaatc agctgtcgag agcgagattt cagtgttagc
    25381 cagggagact acgcggttga tgggatcgat ggtcaacgtg ccgaaatgca gggctttgta
    25441 gggagtcaga gacgtttcct gaataccttt ggtcactgtg gcttgctcat tctgacgcaa
    25501 atgcaagcgt aaacgcgcta acaaaacagc agggggcgtc gttttgagaa tatagtcgca
    25561 ggcgcccatt tccagtgcca ggatgtggtt catatcgcta tcgagagagg ttagaagaac
    25621 aatcggtccg gaccactttg cgcgtaaatc acgacaaatg gtcatgccgt ccttgcctgg
    25681 tagcatgatg tcgagtaaca ccaaatccgg attttctcgc agaatagttt cttcggcccg
    25741 gtcgccgcgc ggctctacgg taacctgcat atcatgtttt gccaggtatg cggcaatcag
    25801 tgcaccgact tccgcatcat cttccacaaa tacgatagtg ttcataacat tcacggtaga
    25861 tataaaaacg tcacaataca ccgcactgtt tttacttact atcaatcgtt tctaaacaag
    25921 gtttcatcag gtattctact gatgacttgt tatggaagtg ttaaggtaaa aagatggggc
    25981 tggtaatcaa agccgcgctc ggtgcgctgg tagtgctgtt gattggtgtt ttagcaaaaa
    26041 cgaaaaatta ttatatcgcc gggctgattc cacttttccc gacctttgcg cttatcgcgc
    26101 attatattgt tgccagcgaa cgcggcattg aagccttacg cgcaaccatc atttttagta
    26161 tgtggtcgat tattccctat tttgtttacc tggcgtcgct atggtatttc accggaatga
    26221 tgcgcttgcc cgctgctttt gttggctctg ttgcgtgctg gggaataagc gcatgggtgt
    26281 tgattatatg ctggattaag ctgcattaac gcagatgacg accgccatca agtgggagac
    26341 tgcgtccggt gacaaagcag ctggtaagta agtaatcgac caggtcgatc acttctttct
    26401 cgccaggcgc ggttttcatc agtgatttat tcagcgcctg ttgtcgatat tcggcatcat
    26461 catgttcatt aaacaggatc agcgatggcg caatagaatt cactttcact tccggtgcca
    26521 gcttgcgggc aaacgagcgg gtcatattat ccagtgccgc tttgcttgca gcataggcaa
    26581 tatgtttgtc gctaccgcgc tccaccacat aatcggtaaa gtgaatgata tcgctggcgg
    26641 cgtgtccgtg cccacgcagt aatctttcca gagcatggtt gagcaggtat ggggtattaa
    26701 cgtggatctg catcatgcaa gccagtacgt cggtcagtgg cgctcccggt ttttccgcca
    26761 tccacgcgct ggcgttatgc aaaatagcac gcagaccatg ggtgcttttt agtacttcat
    26821 cggcaaacgc catcacaccg tcgttggtcg aaaaatcagc ctgaatacac tgcgcacctg
    26881 ccttaatcag tccatcaatg gcgggatagt gcgtccgata gctgacaatc accggttgct
    26941 tttgattaat gaaatgccat gcgagggcga ggccgatgcg gcgacctccg ccagtaatta
    27001 atattggcaa ggactgggtt ttacccatcg ttatctcctt tgctatccaa cgatgggatg
    27061 aacgttatcc caccagcatc caggtcgccg gaactgaggc aattaacagc atgccaatca
    27121 gcaccatttc ctgacggttc agtacattat catgtgtatg cgttttacgc gcgtacagga
    27181 aaaccagcag ccccggcgca tacagaacaa cggataacag caagtgcatt ggaccagagg
    27241 cgtacagtaa ccataagcca taaatgcagg caccgacacc taccgcctta tgcagtggac
    27301 gggttgcgat tttcagcaag aacgcgccga ccaggaaata gggcaccaga atcatttctg
    27361 aggcgatggt cagcaatgtg ttgtaatcgg aaccggtcag ccagatgagc accagacaaa
    27421 tttgcacgca gatgttggtc agccacagtg aagcggatgg cgcagcttgt gcgttctggc
    27481 gcgcaaaaat gcgcgggaat gctttatgag tggctgcaag gaacggaact tctgctgcca
    27541 tgatggtcca gcttaagtac gcgccgcaaa cggaaacgat caaaccggca gcgatgatga
    27601 tttcgcccca cggtcccatc atttccacca tcagaccggc cattgacggg ttacgaattt
    27661 cagccagttc aggacgtgcc accacgccca gcgaaagcag cgttaccagc aaataaacgc
    27721 ccagagcgga gagaaccgcc agcagtgtcg ctttgccaac atcacgctta ttacgcgcac
    27781 gcgcagaaac aaccacggca ccttccacac caatgaaaac ccacagggtg atcagcatgg
    27841 tgtttttcac ctgttcccac acgggtacgc caagtgcaag tccggtgaag tcgagcttga
    27901 aggtatccag tttgaacatc atcatcgcca gcacaacaaa cagacccaac ggcaacaatt
    27961 ttgccagtgt cgccacaagg ttaatgctgg cagcggtttg caccccgcgc aggatcaaaa
    28021 agtgaacaat ccacaataaa gccgatgcac cgacgatcga ttgccaggta ttaccatcgc
    28081 caaacaggcg caattccggc gtgtccgtaa aaaagcttaa cgcggaaaaa acgatcacca
    28141 gataggagac gttggcgatg accgcgcaca gccagtatcc ccatgcggaa caaaagccga
    28201 ttagctcgcc aaacccttcg cgggcatagg taaagatacc gccgtcaagt tcggggcgaa
    28261 tgcgcgtgag gatcagcatg gcaaaggcca gcaataaaat gccagcgcca gtaatacccc
    28321 agccgatgag cagtgctgcc gggctggcaa ctgccgccat attttgcggc agactgaaaa
    28381 cacccgcgcc cagcattgag cttaatacca gcgcggtgag tgcgctcagt cccagtttct
    28441 tttccatcga tatccagtga ttaaaacgtg acaagaagaa taaatattag ggcaaaacgc
    28501 ataaaaatgg agtttgccac tgaagcgcgg gattttacga agagatgtgt ctgcatgcaa
    28561 tggtcgcagg gaaatttatg actaaaaatg cgaaaggcgg catcaagccg ccttatagga
    28621 gtaactgaat agttatttat acagatctgc gctgacggtc aggttattac cacgttcctg
    28681 ccactggcgg gtgatgtggt aatacttagc acctttcttc gcggcacgtt tcgcaacctg
    28741 ataggagact tcggtcatgt tgccgtagtt gccagagaat ttgatgctgt cgaacgggac
    28801 catcatcgct gctgtcgctt tgttcagttc ttcgacttta gtgccatccg ggagcgtgac
    28861 ggtgtaacgc ccgccttttg atgactgggt ttcaaagaag cgaccgactt cagaacttgg
    28921 tgatgcggta gtcgcaacac ccgggatctc aactttcttc gcggcttcgc caccggcagc
    28981 cagagctgca cgtcctgctt cggaatctgc cgggattaca tccgggctct ggacgatacg
    29041 tttcttagca tcttttttat agatgaatgc agtaatacgc tggttaccgc cctggttggc
    29101 atcgatttga cgaacaatgt agaaagagta ggcacctttc gcttttgccg ctttggtgat
    29161 ggcatcattg acttctggct ggctacgata gaagccctgg acggtgaccg tgtcaaacgg
    29221 ttcaatcaga acagcctgat ctttcggcag ttcgacaaca ccgttaatta cgcgattact
    29281 tgtttcttct gctttttcag catcggcttt atagaggtca gcgaccacac gccagttacc
    29341 gctgttacca aaatcagaag tgtcgacaac ataaaaagag gcggcacctt ctttatctgc
    29401 gcgacgagaa acggctttca ccgcttcgcc aatagcatta aaacgaccgg taaccactac
    29461 acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc agttctgttg ctgcgttaac
    29521 ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg gtgttcttaa gcttcataaa
    29581 aataatcctt cgccttgcgc aaaccaggta ctggtattgt tattaacgag aaacgtggct
    29641 gattattgca tttaaacggt gtaactgtct gcgtcatttt tcatatcaca ttccttaaac
    29701 caattttaat tctgctcaaa tgaccgtcta tgcttaaaaa acagccgtat caccatcaat
    29761 actactgaag caactgaatt gtataagtta atttaatgtt aagtagtgat ttgtgccggg
    29821 gcgatgtctc gttttacccg gccgccgaag acaattatca gtctttatcc ggcgttctaa
    29881 ggtgtttatc ccactatcac ggctgaatcg ttaatatttt gcgagttcac gccgaaatac
    29941 tgatttttgg cgctagatca caggcataat tttcagtacg ttatagggcg tttgttacta
    30001 atttatttta acggagtaac atttagctcg tacatgagcg gcttgtgtgg ctcctgacac
    30061 aagcaaacca tcatcaataa aaccgatgga agggaatatc atgcgaattg gaataccaag
    30121 agaacggtta accaatgaaa cccgtgttgc agcaacgcca aaaacagtgg aacagctgct
    30181 gaaactgggt tttaccgtcg cggtagagag cggcgcgggt caactggcaa gttttgacga
    30241 taaggcgttt gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga
    30301 gatcattctg aaggtcaatg cgccgttaga tgatgaaatt gcgttactga atcctgggac
    30361 aacgctggtg agttttatct ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga
    30421 acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt atctcacgcg cacaatcgct
    30481 ggacgcacta agctcgatgg cgaatatcgc cggttatcgc gccattgttg aagcggcaca
    30541 tgaatttggg cgcttcttta ccggacaaat cactgcggcc gggaaagtgc caccggcaaa
    30601 agtgatggtg attggtgccg gtgtcgcagg tctggccgcc attggcgcag caaacagtct
    30661 cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag ttcaaagtat
    30721 gggcgcagaa ttcctcgagc tggattttaa agaggaagcg ggcagcggcg atggctatgc
    30781 caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa
    30841 agaggtcgat atcattgtca ccaccgcgct tattccaggc aaaccagcgc cgaagctaat
    30901 tacccgtgaa atggttgact ccatgaaggc gggcagtgtg attgtcgacc tggcagccca
    30961 aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc ttcactacgg aaaatggtgt
    31021 caaagtaatt ggttacaccg atcttccggg ccgtctgccg acgcaatcct cacagcttta
    31081 cggcactaac ctcgttaatc tgctgaaact gttgtgcaaa gagaaagacg gcaacatcac
    31141 tgtggatttt gatgatgtgg tgattcgtgg cgtgactgtg atccgtgcgg gcgaaattac
    31201 ctggccggca ccgccgattc aggtatcagc tcagccgcag acggcacaaa aagcggcacc
    31261 ggaagtgaaa actgaggaaa aatgtgcctg ctcaccgtgg cgtaaatacg cgttgatggc
    31321 gttggcaatc atccttttcg gctggatggc aagcgttgcg ccaaaagagt tccttggaca
    31381 cttcactgtg ttcgcgctgg cctgcgttgt cggttattac gtggtgtgga atgtatcgca
    31441 cgcgctgcat acaccgttga tgtcggtcac caacgcgatt tcagggatta ttgttgtcgg
    31501 agcactgttg cagattggcc agggcggctg ggttagcttc cttagtttta tcgcggtgct
    31561 tatagccagc attaatattt tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt
    31621 ccgcaaaaat taaggggtaa catatgtctg gaggattagt tacagctgca tacattgttg
    31681 ccgcgatcct gtttatcttc agtctggccg ggctttcgaa acatgaaacg tctcgccagg
    31741 gtaacaattt cggtatcgcc ggtatggcga ttgcgttaat cgcaaccatt tttggaccgg
    31801 atacgggtaa tgttggctgg atcttgctgg cgatggttat tggtggggca attggtatcc
    31861 gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc ctgcatagct
    31921 tcgtgggtct ggcggcagtg ctggttggtt ttaacagcta tctgcatcat gacgcgggaa
    31981 tggcaccgat tctggtcaat attcacctga cggaagtgtt cctcggtatc ttcatcgggg
    32041 cggtaacgtt tacgggttcg gtggtggcgt tcggcaaact gtgtggcaag atttcgtcta
    32101 aaccgttgat gctgccaaac cgtcacaaaa tgaacctggc ggctctgatc gtttccttcc
    32161 tgctgctgat tgtatttgtt cgcacggata gcgtcggcct gcaagtgctg gcattgctga
    32221 taatgaccgc cattgcgctg gtattcggct ggcatttagt cgcctccatc ggtggtgcag
    32281 atatgccagt ggtggtgtcg atgctgaact cgtactccgg ctgggcggct gcggctgcgg
    32341 gctttatgct cagcaacgac ctgctgattg tgaccggtgc gctggtcggt tcttcggggg
    32401 ctatcctttc ttacattatg tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg
    32461 gcggtttcgg caccgacggc tcttctactg gtgatgatca ggaagtgggc gagcaccgcg
    32521 aaatcaccgc agaagagaca gcggaactgc tgaaaaattc ccattcagtg atcattactc
    32581 cggggtacgg catggcagtt gcgcaggcgc aatatcctgt cgctgaaatt actgagaaat
    32641 tgcgcgctcg tggtattaac gtgcgtttcg gtatccaccc ggttgcgggg cgtttgcctg
    32701 gacatatgaa cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg
    32761 acgagatcaa cgatgacttt gctgataccg ataccgtact ggtgattggt gctaacgata
    32821 cggttaaccc ggcggcgcag gatgatccga agagtccgat tgctggtatg cctgtgctgg
    32881 aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc gatgaacact ggctatgctg
    32941 gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat gctgtttggt gacgccaaag
    33001 ccagcgtgga tgcaatcctg aaagctctgt aaccctgacg gcctctgcgg aggccgtcac
    33061 tctttattga gatcgcttaa cagaacggcg atgctttgac ctcccgctgt ttgttcaagc
    33121 gcaattttga caataattgt caacggcacg gaaagcagca tacccaccgg tcctaacaac
    33181 catccccaaa aaatcaacga caaaaatacc accaatgtgg aaagccccag cccacgcccc
    33241 atgatgcgcg gctccagaat attgccgaag acaagattaa tcagcagata tcccgccagc
    33301 accagcaacg cttcgtagaa gccattaaac accagtacct gagcgatagg ggggattgcc
    33361 gcgagaactg aaccaatatt ggggatgtaa ttaagcgcaa aggccagcaa tccccagaca
    33421 aaagcgaagc gaacatcgag tgcggcgagc atcgcccagg cgaccaggcc ggtgatgatg
    33481 ctgatggctg ttttcagcac cagataatga gacacgctgt caatcgcgcg ctgaatcgcc
    33541 gccatccctt caaccggacg cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact
    33601 tcgagcaaca taaacagcac cgtcagcagc aataaaaaga ttgatgacat ggcattagat
    33661 aactgcgtca ataagttggt gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc
    33721 agttggtcaa ctgagacatc aatccctacg cgttgcaaca acggttcaat cgcttgcagc
    33781 ggcgtcataa tagagttgcg atattgcggt aacgtccgcg tcaactcatt gagcgcggac
    33841 cccagataag ctaacagcaa caccatcgcc atcacgatga tggtcatcaa aatcgacact
    33901 gccagtacac gcggcacacg ccagcggacc atgtgttgca ccagcgggtt aagaataaca
    33961 gcaataaata atgcgagaat aaacggcacg ataatctcgg cggcaaaacg gataccgcag
    34021 agaataatga ccagcattcc caacataatg acgattttta ggccattgag cgtgatgatc
    34081 ggctttgcca tgcttgtccc cgttttttct tcctttgcta ataataatgt ctttttcgct
    34141 ccttcatcat aacgaaaatc aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat
    34201 tcttaagcta tgatacaaat caggcgtgtt caactaccga ggacaattat catccgcgat
    34261 gacgagaagc aacactgcgg ataattgtaa tattatggac aatttgttca ggacgttatt
    34321 ttctacattt acccatcttc gtacctcatc cactatcttg ctcgtaggtg agcagcactg
    34381 gcgcaacgcg ctttagtcct ttcttctgcc taagcttgcg ctatgcgcgg ggcttgatca
    34441 ctttactttg gtttcgctga attaagcgaa aattaaaata attctcttgc aggagaagga
    34501 caatgtatat ttattggatt ttattaggcc tggctattgc tacggaaatt accggtacgc
    34561 tgtcaatgaa atgggcgagc gtcagtgagg gaaatggcgg ctttatttta atgctggtga
    34621 tgatttctct gtcgtatata tttctctctt tcgccgttaa aaaaatcgcc ttaggcgtag
    34681 cttatgcgct gtgggaaggt attggtattt tatttattac cttgtttagc gttttgttat
    34741 tcgacgaaag tttatcgctg atgaaaattg ccgggttaac caccctggtg gccgggattg
    34801 tgttgatcaa gtcaggtacc cgtaaagcgc gtaaacctga actggaggtg aaccatggcg
    34861 cagtttgaat gggttcacgc cgcctggctg gcattggcaa tcgtgctgga aatcgttgct
    34921 aacgtctttt tgaaattttc tgacggcttt cgtcgcaaaa tatttggctt gctctctctg
    34981 gcggcggtgc tggctgcctt tagtgcgctt tctcaggccg ttaaagggat cgacttgtct
    35041 gtcgcttatg cattgtgggg cgggtttggt attgccgcca cgttagccgc aggttggatc
    35101 ttgtttggtc aacggttaaa tcgtaaaggc tggattggcc tggtgctgct gttggctgga
    35161 atgatcatgg tgaaacttgc ctgatgaaca cgctgcccgc gctgtcgggc agcgtttgaa
    35221 cattattttt gcgacagttg atccagcttg tcgcgaaaac cggtaacaga aatggcccgg
    35281 ttatcggcgc gccagcgatc tttcgcggca ggagccgagc tttgcacccc aattaattgc
    35341 cagccgtcat cggtatgcaa catcagaggc gaaccgctgt cacccggcaa ggtatcgcac
    35401 tgatgcgaca tcaccgacgt ttgcgcccag ccagtcactt cacagttttg atgactgtac
    35461 aacgtatcga gatgatcttc agggtagccc gcctgagtca ctttacgacc agccgctttt
    35521 aatgcggcgg taagcgcggc tttatctccc tcaaataacg gcaacggtgt aatgccagaa
    35581 gggggattac gtagcacaat caaaccgaag tcccacggcg cagctgcggg aggtacaatc
    35641 cagccatccc catctgcttt taaccgcttt cccagtgttg gatcaacgcg gccttctatg
    35701 tcgtggatct catagcgcca aagaccttta tttgacacga aacgcagcgc taccgcttta
    35761 tcggctttac cctttggcgg cgtcaataaa cagtgtcctg ccgttaatgc cagattgggt
    35821 gcaatcagcg tcgccgtaca taaattgccg ctggccgttt ccagttggcc aaccgcatcc
    35881 cacggtgatt gggtcgtgtc attcactggc acacgatcat catgaccaaa aaacagggtg
    35941 ctgacctcat cgtttgccga tttggcaacg tctggtttat ctgcaaacac aaaagcagac
    36001 gtcaaactaa ttgcacccaa cactacagca atggttgtac gcatatcaca ctctggtggg
    36061 taattatgat tattaaaagc aaaccctcat aaatactata gacgggacgg gatgaaagtg
    36121 ggagtaaaat cagataacta cgatcaggaa agataaaaac gggcggcaat cagtgcgcat
    36181 aaaataagga tgataagaat gaactcaaag cgataacggc ccaccatact accctccgct
    36241 aaaggcggtg ctaaacgtta cccgcttagc accgtaattt tcaggcgcga gggggcgcgc
    36301 cagcattact gttgaaaact tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat
    36361 ggctgtgttt cttggcgtgt ttcttcgctg cctgcgcttt ttgttcagga gctttctgtt
    36421 cagctttggc atttttatga tgctttttag ccgcctgcgc tttctgggca ggtgctgctt
    36481 tatgctgttt tttatgatgt gtggttttcg caggcgctgc tttggtggtc gtcgcagtcg
    36541 gagcaggtgt ggtcgcagtc tctgcagcaa aggcagcaga agacagaccc atagcagcgg
    36601 caacaaccag agctaatact tttttcattg tcataccctc aatttgtttt ttcatttaac
    36661 cccactgcgg ggccgttgaa ataactatat ccctgggaat ggcagacttc cgtgagtggt
    36721 tggtttcagc gtgtaacgat atgtacaaac gctgaataaa ttacagcatt gataataacg
    36781 tattgtgact taaggaaaat ttagctacca ataaagtagt cttgatcgtg ttaactattt
    36841 tactacttac aaagcggtag aataactgcg catcagtaat aaatgacaca cagcaaaatg
    36901 aatccgttta tttgggtact tataatcctg atgacgctag atgcgctgcg ggaattggct
    36961 ggcgcttcgt ctattttagg atggctatta acgttggttt gagcaggcaa taagtccgga
    37021 cgggtattta cctcagtccg gacttatttt ttcaggcgtg cagacgatga tgcaaacgcg
    37081 tcccgaccag cagagcaatg accagcatca gagcaataaa tgcgccgacg ccgttccagc
    37141 catagttatg ccagaaaaca ccacccagcg tcccggcaat actcgacccc agatagtaac
    37201 tgaacagata cagcgaggag gcctggcctt tagcgcgttt tgcgcggggg ccgatccagc
    37261 tgctggcaac ggagtgggct gcgaagaatc cggctgagaa gagtaacatt ccggcaaaga
    37321 tcagccacag cgagctgaat aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa
    37381 acaacatcac cggaccgcgt ccatagcggg cggtcatggt tccggctttg ggtgagctcc
    37441 atgtaccggt caaataagcc agcgataata agccaaccac ggcctgactg acatgccagg
    37501 gggaaagcat caacctatag ccgatgtaat taaacagcgt gacgaacgac cccatcagca
    37561 aaaagccttc tgcgaacaat aacggtaatc cccggtcacg ccagtgcaga cgaaagttga
    37621 taaataacgt cttagggcgc agcgaagtcg ggcgaaaatg gcgtgattca gggaggattt
    37681 tccagaacat caacgccgag gccagcgcga aacaaccgat tgccgccaga gcaattcgcc
    37741 agttaaaaaa gtccgtgaag acaccgctaa ttaagcgtcc gctcatgccg ccaattgagt
    37801 tgccgctgat atacaacccc attgaaaagg ccacgaaact agggtggatt tcctcgctaa
    37861 gataagtcat gccaacggct gccacgccac ttaacgaaag cccaatcaag gcgcgcataa
    37921 tcaaaatgcc gtgccagctg gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca
    37981 gtagcgccgt gaccatcact ggtttgcgac caatggcatc ggatagcggg ccagtaaaca
    38041 gcaaaccgat agccaacatc gccgttgaaa tggacagtga aatactactg ttcgcaggcg
    38101 ttaaaccaaa ctcctgcgaa agcaccggaa ggataggttg cacacaatag agaagtgcga
    38161 aggttgccag tccggcagag aaaagcgcca gggtgacgcg cataaattgc ggcgtaccgc
    38221 gtttaataaa atgatttggc tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc
    38281 catcaacagt tgtagtacgg ctcacttgaa atccttgcta aatatgcctg tggatcaggc
    38341 ttatgaatag attaggaaaa tcgaattgtt ctgtctaata tattaataat ctcaaataag
    38401 atgttttaaa tatgaatatt gaacttcgtc atctgcgtta ctttgttgct gttgcagaag
    38461 agctgcattt cgggcgcgcc gctgcccgcc tgaatatttc gcaaccaccg ctaagtcagc
    38521 agattcaggc gctggagcaa caaattggtg cccgactgct ggcacggacc aatcgcagtg
    38581 tattgctgac ggaagcagga aaacagtttc ttgtagatag tcggcaaatc ctgtctatgg
    38641 tggatgatgc tgccgctcgc gctgaaaggc tgcatcaggg cgaagcgggg gagttgcgca
    38701 ttggttttac ttcgtccgct ccttttattc gggcggtgtc cgatacgtta tcgctgtttc
    38761 gccgtgatta tcccgatgtc catttacaaa cccgcgaaat gaacactcgc gagcaaatcg
    38821 ctccgctcat tgaaggaacg ctggatatgg gattgctgcg taacacagcg ttaccggaga
    38881 cgcttgaaca cgcagtcatc gtccatgaac cgcttatggc gatgatcccg cacgatcatc
    38941 ccctggcaaa taacccgagt gtaacgctgg ctgaactggc gaaagaaccc tttgtctttt
    39001 ttgatccgca cgtcggaaca gggctgtatg acgatattct cgggctgatg agacgttaca
    39061 atttgacgcc cgtcatcact caggaggtgg gtgaggcaat gaccatcatc ggtctggttt
    39121 ccgctggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa
    39181 tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga
    39241 aacatcatga acaaagtccg gctgcgcgta actttcgtat tcatctgctg aatgctctca
    39301 ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta
    39361 aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg
    39421 aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa
    39481 gcaaaccaac gcgggcgcag tttatcgcct gattgatcag cttggtccag tctcgcgtat
    39541 cgatctttcc cgtctggcgc aactggctcc tgccagtatc actaaaattg tccgtgagat
    39601 gctcgaagca cacctggtgc aagagctgga aatcaaagaa gcggggaacc gtggccgtcc
    39661 ggcagtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg
    39721 cggggagatt ttccttgctt tgcgcgatct gagcagcaaa ctggtagtgg aggagtcgca
    39781 ggaactggcg ttaaaagatg actcgccatt gctggatcgt atcatttccc atatcgatca
    39841 gttttttatc cgccaccaga aaaaacttga gcgtctgact tcgattgcca taaccttgcc
    39901 gggaattatt gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa
    39961 agagatgccg ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca
    40021 tgatatcagc gcatggacga tggctgaggc cttgtttggt gcctcacgcg gggcgcgcga
    40081 tgtgattcag gtggttatcg atcacaacgt gggggctggc gtcattaccg atggtcatct
    40141 gctacacgca ggcagcagta gcctcgtgga aataggccac acacaggtcg acccgtatgg
    40201 gaaacgctgt tattgcggga atcacggttg cctcgaaacc atcgccagtg tggacagtat
    40261 tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac atggacaacc
    40321 gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatttactgg caaaagacat
    40381 cattaccggg gtaggcgcgc atgtcggtcg cattcttgcc atcatggtga atttatttaa
    40441 cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt
    40501 catctcggac agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga
    40561 gagtactcag ttttctaacc agggcacgat ggcaggcgct gcgctggtaa aagacgcgat
    40621 gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc
    40681 aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt
    40741 attttcctgg tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct
    40801 ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag
    40861 cgttagcctc ccagggaaaa acggttgcgg gatataaacc cgtagcgaag gggagcaaag
    40921 agacacccga agggctgcgt aataaagatg ccctggtgtt gcagagtgtt tcaaccatcg
    40981 aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc
    41041 acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag
    41101 tcgatcatgt cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc
    41161 cgctctctga atgggtggtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg
    41221 aaggttgcat taaccatgca ctgctaacag ctcaggcgat tgccaacgac gggctgccgc
    41281 tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg
    41341 atgtgctcgg taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg
    41401 ccgaacagcg tgaactgggg caatacatcc gcttagctat gcttcgcagt gtgctggcgg
    41461 tagatagagt cacggtgtaa cgtccgcgaa attaccgacg caattacgca ggcaatcaat
    41521 aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt cgacatagtc
    41581 ggcgcgtgcg tggtcgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta
    41641 atttcttcgc catcaggtaa ccataatccc aggctacgac catacaacat gccaatggca
    41701 agaccgataa atagcgtcgg tgtaaagaca ccaccgggcg cgccagaacc actactcgcc
    41761 agcacggcac acagtttaca gaggaagatc ccggcaatga tcattaacag tggcggagcg
    41821 gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa
    41881 agaagaccca cgatcaaccc gcccagtgcc agttgccagg gcggcgcaag cttgagactc
    41941 acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc
    42001 agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac tgaaagttgt
    42061 acgctgtaga gtaatgcgtc gctatgatta ataagattgc taaccagcaa cgccacgacg
    42121 gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct
    42181 ataaataaac taccagcaag cggggcgcga taggccgcag ccattcccgc cgcagctcca
    42241 caggcgatcc ataatttcca ctcctggcgt ggcgtaaaac gttgggcaaa acacgaggct
    42301 gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact
    42361 accagcagag aggcaagcga tttaaccaga cttgctgcgt aatcaaactg accatcggtt
    42421 tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gctgggtaaa tttctgccag
    42481 cccatcagca acaaacccgc cgccagtccg ccgagcgccg gggttagcag ccgtcgccac
    42541 ggggaaagat ttgtcgctgc attgaccaga ctgccagagt cattattgag gaacagccac
    42601 tccagtagca gcatcgcatg acgaaacccg gcaacggcaa aggccgcaag aataccgacg
    42661 attgttgcga taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga
    42721 tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccagc
    42781 cgcaaaagca gtgtgtgctt taagagataa aaaaaacgtg acactgtaac ctattaattg
    42841 ccgaattgcg tgtaatcgtc accatccggc aataatacgg tgatcctatc gaaacagcgg
    42901 tttaaccgcg acaggaatca acagttgtga ttgccattgc gccagcgtta agcgagcgag
    42961 ttcacccagt gcacggtaga aagggtgttc tgctttttcg ataaaaacgt caagaaaacg
    43021 tgttgaccac ggaaaaaggt gccatgccag caattcttcg cattctgtct ggcgaccatt
    43081 ctccgccaac cacgcagcca tcagcagcag tgaaccaaaa ttatcttccg gttcgttttg
    43141 cttcatttcg aactgaatgc ctttctcgcg catccactga cgaagtgcca atgttgaatc
    43201 gccaaacagc acagattcgc gatccagcca gaccgatccc cacggcggag acggcagtgc
    43261 ccacgggccg acaaacaaac gctgccaggc ctgggcgtga gtctcttcac attgtgtctg
    43321 aaaagccgtc accagcggcg ctaatgacgc ttctggtaga ggccactgag tttcccagcc
    43381 gtcattggtg agtaccgcaa ccagaggtgc ggcttccgca ctctctggag cgtaataaaa
    43441 cagcgcgccc aacacgcgcg ccgcaacaga aaaattatct tgctgtgaaa aatgggtcat
    43501 tcctaacatc ctgaaatgtg cgggttgccc cgcaccaatt tgttaacctg caatagccat
    43561 tcctaccgtc atgtgcaggc cataaaagag cacacgcccg atcatctcac cgccaagaat
    43621 gaggattagc cccagaacaa gtccggcaac gtgaggttca cggcgacgaa tgagcggaca
    43681 aagccagcat ccaagacctg cgcataacaa taccacacgc cagacctgta aggaggcgta
    43741 atctggtacc agtgcgctgg cttgttgtac ggatgagtga atagacgcca gagacaaacc
    43801 ttgcagcaca atcaacccgg cacaagcgat cagcgccaga atgctaatga tggcaaatgg
    43861 tgtggtatta aatgtaacgc gggctgcacg cagaattgtg gcagcaagta ttggaccgct
    43921 caacaatacc gtcaggaaga aagccagcgt agtgtaaccg ttatgccagg ttggcacagt
    43981 gtcgatttga tacacgcagg tcatcatcca gacgaaaatg atgccgagtg ccatgctgac
    44041 aagtaaccag agtttcccca acgcttgcgg cattttaccg atgaccgcca ccagccacca
    44101 taatccgcca acggcaaaaa atatggaacc ggcagcaatc tcgttactca gaccagaagc
    44161 tccgattcta ttcagcgagt taaacgcccg cagtggcgat cccaggtgca tgacagaggc
    44221 aataaacccg atgcccatca acagccagag gaaaaacatg ccacgaataa tgtgctgacg
    44281 gtctgcgtca tttttggcgg cgaaccagcc aattccgcta acaatcagcg cgcctactac
    44341 gcattgaccc aatacggtaa agataaccag tggccattca tgccatccat ttcccatttt
    44401 acacttcctc cggattagcc agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt
    44461 taggtttgat aacgatattg ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag
    44521 ccagagtgcc gtgcttctgg cgcaattctt caattggacc gaactcaagt gcacgcagtg
    44581 ggcaggattc gacacatatg ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc
    44641 acttcgtcat gtgccctttt tcagcattgt actgtggcgc gccgtacgga caagccatgt
    44701 ggcagtagcg gcagccgata caaacatctt catcgaccac cacaaagcca tcttctcgtt
    44761 tgtgcattgc gccgctgggg cagactttgg tacacgccgg atcatcgcag tggttgcacg
    44821 aaattgagag atagtaggca aagacattct gatgccagac gccattatct tcctgccagt
    44881 cgccgccagc atattcataa atacggcgga aactgacttc cgggccaaga tctttaaaat
    44941 ctttgcacgc cagttcgcag gttttacagc cagtacagcg gctggaatcg ataaaaaatc
    45001 catattgtgt ggtcatgggc tactccttaa accttttcga tctggacaag attgctgtgc
    45061 gacgggtttc cctttgccag cggtgaaggg cggtgagagg tcagaatacc gttaactgca
    45121 gtggataggt cccccgtagg ggatcgttcc aaccgtcgaa acctggggta tacgctggca
    45181 gtggatgaat aatttcatct tttttcaatt cccaggtatc cgcaattgtc gccagtcgtt
    45241 cagaataaat ttcgattttt cccgaaggtg ttttcaacgg gtttgcctgt ggatctacac
    45301 ggaatgcgcg gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg
    45361 ttttcatctc ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat
    45421 atttgatcca ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta
    45481 agcgtttggc gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg
    45541 ttgcgggttg ggcgaggatc acatagccca tattcccggc agattcatga gagataaggt
    45601 cttcttgctc tgttggcatc aggtcgggta acaggatatc gcaatactta gccgaggcag
    45661 tcatgaagtg gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat
    45721 gggtgtgatt gatgtcgcca tgctgattga tcaatgtgtt actggcgtag caccataaaa
    45781 acttgatggg gacatccagt ttttcttttc ctcgaacacc atcacgggtc gcggtcattt
    45841 ccgtaccatg gtcgatggca tctgtccatg taaagacgga aagccgctca tccgcgtttc
    45901 tgacgggtta tttccgaaca taacgaccag tttcgtattg gcgatatcat ccgggctgtt
    45961 gccatcattg gcaccgaaca tataactcat tgcggcactg atctgtgcgg tactgtagct
    46021 gccatagcga ctgagaaaac caccgcaaga gttcatcaga cggtacggga cgtttgagtt
    46081 ggtgatgttt ccgccatcta cgcctgttcc atagagaaca tgtacagcct cattgccgta
    46141 atcttttagg atccgccgaa gattatcact gatggtatcc agggcttcgt cccaacttat
    46201 ccgttcaaat ttaccttcac cgcgcttgcc gacgcgcttc atgggatatt tcaacctgtc
    46261 aggatgattc atccgtcggc ggatagagcg cccgcgtaaa catgcccgaa cctgatgatt
    46321 accgtaaacg tcgtcacctg tcgtatcaga ctccacccag tacacggtgt catctttcac
    46381 atgcaaacgt aacagacagc ggctcccgca gttaacggtg caggaactcc agaccgcttt
    46441 ctcttctacc ggtgcctgtg ccgctcggac catttgggaa aacggcaaag tgaaagcact
    46501 gcttgccagc gcgagactgc caagtgcgga ggttttcatc agacttctac gactgatttc
    46561 agccgacatg agcgcctctg tgttatggat tttcatcatt actcacttat tgcttttcaa
    46621 acaaaatgtc atgtcaaaat ttatggccgt agtgggttat attttttcga tctcgaccag
    46681 attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg cgcagagtgg ttagcgtatt
    46741 cacacagccg ccgtggtcga ttttatcgcc agacatattg gcctcgtgcc aggctccctg
    46801 gcccatagca ctaactccag ggagaatgcg tggtgtcact ttggctggta gccgaacttc
    46861 gccacgatgg ttaaacaccc gcaccatatc gccgttggca atcccacgtt tctgtgcatc
    46921 tatagggttg atccacactt cctgacggca ggctgccttc aggacatcaa tgttgccgta
    46981 ggtcgagtgc gtacgggatt tgtaatggaa accaaacagt tgcagtggga aggtactacg
    47041 ttcaggggag tcccaccctt caaaggttga ggcataaacc ggcagtgggc ttatcacttc
    47101 atctttttcc agttcccagg tacgggcaat ttccgccagc ttgctggaat aaatttcaat
    47161 cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg tcacgaaatg ctttgtaggc
    47221 gacaaaatga ccattgggat ctttacgctt atagataccc atttttttta attcgtcgta
    47281 agacggtaac gccggatctt tggcaagcat tttggcgtac agatgttgta accattgttc
    47341 ttgcgtgcga ccttcagtga acttttgata gacgtcaggt ccaagacgtt tcgcgacttc
    47401 actcaggatc cagtaaattg gtttgcgttc gaatttttcg ctggtgacag gctggaggaa
    47461 aatgagatat cccatgttac cggcgtagtc gttaggaata atatcttcct gctcaacggt
    47521 cattaggtct ggcagcagaa tgtcggcata ttttgccgat gaggtcataa agttttcgat
    47581 gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt tcatgcgttt tgttgatgtc
    47641 agaatgctga ttaaccaggg tatttcccgc gtagttccag atgaacttaa tgggcacatc
    47701 tagtttatct ttgccccgga cgccgtcgcg gattgccgtc atttgcgggc catgatcgat
    47761 agcatctgtc caactgaagc aggagattga cgttttgacc ggattatcca gcaccggcag
    47821 gcgttcgatg gtaatggtgt aggtcgattc acgcgcacca ctatttccgc cgctgatgcc
    47881 gacattgccc gtcaaaatag gcaacatagc aatagcgcgt gccgtcagct cgccgtttgc
    47941 ctgccgttgc ggcccccagc cctggcagat ataagcgggt tttgctgtgc caatttcacg
    48001 cgccagtttg atgatacggt ctaccgggat acctgtaatt tgcgaagccc actgcggcgt
    48061 tttcgctgtt ttatcatcac cttcaccaag aatataggct ttatagtgac catttttggg
    48121 tgcatctgcg ggtaaggttt tttcgtcata gccgacgcag tatttatcga gaaaaggttg
    48181 atcaacgaga ttttcgttaa tcaataccca ggcaataccc gcaaccagcg cggcatcggt
    48241 gcccgggcga ataggaagcc attcatcttc acgaccagca gccgtatcgg tatatcgcgg
    48301 atcgataaca atcatttttg cgttcgattt ctcgcgcgct ttttcaagaa gataagtgat
    48361 gccaccgccg ctcatgcggg tttcagccgg gttgttacca aacatcacga ccagcttgct
    48421 gttttcaata tccgtggtgc tgttgccatc attactgccg taggtgtagg gcatggcaca
    48481 ggaaatttgc gcagtgctgt aggagccata ctggtttagt gaaccgccgt agcagttcat
    48541 caggcgtttg accgccgagg ctgatggaga agagcgggtc atattgccgc caacaatccc
    48601 cgaagagtac tgaatatata cagcctcatt gccatattgt tcgacggttt ttttcaggct
    48661 actggcgata gtatccaggg cttcatccca gctaatccgt tcgaatttgc cttcgccgcg
    48721 tttgcccacg cgtttcattg ggtaattcag gcgatcggga tgattaatac gccgacggat
    48781 ggagcgaccg cgcaaacagg cgcgtacctg atggttgcca tacttgtcgc tgccggtgtt
    48841 gtcagtttcc acccaggtca cttcattatc tttaacatgc agacgaagtg cacagcggct
    48901 accacagttg acggaacagg caccccagac cactttttcg ctggcctgtt gtaccgctgc
    48961 tgctgcactg cgcagggtaa acggcaaaga aaaaccgcct gcagccagcg ccagagaacc
    49021 tatcgcagta gatttaacga gtgttctgcg gctgatgccc accatttgtt catttttgga
    49081 cataactcac tccctgttct ttatcgttat ataaatgttt atatattgaa tatttagcgc
    49141 gctaacaata gagggaatct acccatttgg ggttaataat tattaatcca tatcaataga
    49201 agggtatgag taataaggtg gggttatgtt gaatgttcaa atcgccggat gtgtcgcatc
    49261 cggcgttcag tcgataatgt gttactgcgg ttcggcaggc gcgccatcct gggtagactg
    49321 cgcgggagca gagacgttac cgctggtggt acgggtgtag agaattttat gcgtatcatt
    49381 agcgcaatgc ccgacgacct gggaatcagg ctgatcaacc tggtcattgg gtacaatact
    49441 taacgtgaag ctgctttcgg gtacgccatt attgataatg cgctgtgata tatcgctctg
    49501 tatgcgctca caggatcccg gcgcggcgag taccgtgggt gaggcgaggg cgagcagaag
    49561 cgcggcacag taggttgagt gtttcatcat aagctcctta cgcgaagata acttctttaa
    49621 gcatagcatt taacgtgtaa agtactgtat ttgctactat gattgagaat catctctact
    49681 ctcaggtgac tgttgtgaaa tacaaattac tgccatgctt actcgcgata ttactcacag
    49741 gatgtgaccg cacagaggta acactttcat ttaccccgga gatggccagt ttctctaatg
    49801 aattcgattt tgatccgctg cgtggtcccg taaaagattt cactcagaca ttaatggatg
    49861 agcaaggtga agtgacgaaa cgtgtttcgg ggactttgtc ggaagaaggc tgttttgatt
    49921 ctctcgaatt actggatctg gaaaataata ccttggttgc cctggtactg gatgccaatt
    49981 attaccgtga tgccgagacg ctggagaaaa gagttcgttt gcagggaaaa tgccagctag
    50041 cagaattacc ttctgccggg gtgagttggg aaaccgatga taatggtttc gtgattaaag
    50101 ccagcagcaa acagatgcag atggaatatc gctatgatga tcaggggtat ccgctgggta
    50161 aaaccacgaa aagtaacgat aaaacattat ctgtcagcgc cacgccatca acggacccga
    50221 tcaaaaaatt agattacact gctgttactt tactgaataa tcaacgggtt ggtaatgtaa
    50281 aacagagctg tgaatatgac aatcacgcca atccggtgga ctgtcagcta atcattgttg
    50341 atgaaggagt aaaacccgcc gtcgaacggg tttataccat caaaaatacg atcgattatt
    50401 attaatacta ttgtgcggtc ggcttcagga gagtctgacc cggtgttttg tgctcagcca
    50461 gatactgatg ctggaatata cacatgcgaa tggcattacg atattgacca ttaataaaga
    50521 actcatgcat caattcacct tcaaccgtaa agccaagctt gcggtaaatg tgaatcgctt
    50581 tttcattctc tttatcaacg atcagataca gcttatagag attgagaacg gtgaagccat
    50641 agtccattgc taatttggcg gcacgggttg ccagaccttt cccctgatac tccggggaga
    50701 taattatctg aaattctgcg cggcgatgaa catggttaat ttccaccagc tccaccagac
    50761 cggctttttc gccgtcacat tccaccacaa agcgccgttc gctctgatcg tgaatatgct
    50821 tatcatacag atcagagagt tcaacaaagg cttcgtaggg ttcctcaaac cagtaacgca
    50881 tcacactggc gttattgtcg agttgatgta catagcgtaa atcttcacgc tccagcgggc
    50941 gtagcttaac actgtgggcg cttggcataa cgtgtcctta cattccttaa atcaataaca
    51001 ggttagggga taataacgcg gccagttcga cggtccaggc agcgcaaagt atttggctcc
    51061 cagtaggcat tgatgttggc gctttgctca catttatcgc ggttatcaaa agcagcgtcg
    51121 gctttatccc actctttttc agtgcgttta ttcactttct ggcgcagatt acgcgtgtca
    51181 ttccattgct ctttttccat agcggcgcgc tggcggcttt gtgcactgtc gccagactca
    51241 atcaccagtt tgttagtttc ggcatgaaca gttgtgctca ataccagtgc gcaaggcagc
    51301 agaaaagcga gcaggccgat tcgtttgctg agagtgattt tcataattca ttccctgtat
    51361 gaatgattaa agctgattct acaccatcca ctgcgaacgc aaaacgttca aggagggtgt
    51421 ttatattgat gatattatgt cgccctataa ctatacatga tgtcaataag tgacaaagat
    51481 gattaaaaca acgttactat tttttgctac tgcgctgtgt gaaattattg gatgctttct
    51541 gccctggttg tggttaaaac gaaacgccag tatctggctg ttgcttccgg cggggatttc
    51601 actggcactg tttgtctggt tgttaacgtt gcatcctgcg gcgagtgggc gtgtttacgc
    51661 ggcttatggt ggcgtttatg tttgcacggc gttgatgtgg ctgcgcgttg tggatggcgt
    51721 gaaactgact ctttatgact ggacgggggc gttaattgcg ctttgcggca tgttgatcat
    51781 tgttgcgggc tgggggcgca cgtaggaaca taaatccatt ttatcaataa gatacgagga
    51841 agtgtcagct gacaaaaggt attctatttc atcttttgtc aaccattcac ggcgcaaata
    51901 tacgcctttt tttgtgatca ctccggcttt ttttgatctt catacttgta tggtagtagt
    51961 tcagttgcgt agatttcatg catcacgaca agcgatgcaa gaaatcgaac atgaagatcg
    52021 taaaggctga agtttttgtt acctgtccgg ggcgtaattt cgtcacatta aaaatcacca
    52081 ctgaggacgg tattacgggc cttggggatg ccaccctaaa tggacgtgag ctttccgtgg
    52141 cctcttattt gcaggatcac ctttgtccgc agcttattgg tcgcgatgca caccgtatcg
    52201 aagatatctg gcagtttttc tataaaggtg cttactggcg tcgcggtccg gttacgatgt
    52261 cggccatttc agcggttgat atggcgctgt gggatattaa agccaaagct gccaacatgc
    52321 cgctttacca gttactcggc ggcgcgtctc gtgaaggggt gatggtttat tgccatacca
    52381 ccggtcacag tattgatgaa gctctggatg attatgcccg tcatcaggag ctgggattca
    52441 aagccatccg cgtgcagtgc ggaatccctg gtatgaaaac cacctacggc atgtcgaaag
    52501 gtaaaggtct ggcttatgaa cccgcaacca aaggacagtg gccggaagag cagctgtggt
    52561 cgacggagaa atacctcgat ttcatgccga aattgtttga cgcggtacgt aacaagtttg
    52621 gatttaatga acatttgctg catgacatgc accatcgctt aacgcctatt gaagcggcgc
    52681 gctttggtaa aagcattgaa gattatcgca tgttctggat ggaagacccg acgcctgcgg
    52741 aaaaccagga atgcttccgt ctcattcgcc aacataccgt cacacccatc gcggtgggtg
    52801 aagtcttcaa cagcatctgg gactgcaaac aactgattga agagcaactc atcgattata
    52861 tccgcaccac gctgactcat gcaggcggaa ttaccggtat gcgtcggatt gccgattttg
    52921 cttcgctgta tcaggtacgt actggctcac acggtccttc cgatttgtca ccagtctgca
    52981 tggctgcggc gctgcacttt gatctgtggg tccccaattt cggtgtccag gaatacatgg
    53041 gttattccga acaaatgctc gaagtcttcc cgcacaactg gactttcgat aacggctata
    53101 tgcatccggg agaaaaaccg ggtcttggca tcgaattcga tgaaaagctg gcggcgaaat
    53161 atccctatga acctgcttat ctgccagtcg cacgtctgga agatggcacg ctgtggaact
    53221 ggtaaggagt aaggtaatga aaagcatttt aattgaaaaa ccgaatcaac tgtcgattat
    53281 cgaacgtgaa atacccaccc cgtcagcggg tgaagtacga gtaaaagtga aacttgccgg
    53341 aatttgtggt tcagatagcc atatttaccg tgggcataat ccttttgcga aatatccgcg
    53401 cgtcattggt catgaattct ttggcgtcat tgatgcagtg ggtgaaggcg tggaaagcgc
    53461 cagagtcggt gaacgcgttg ctgtcgatcc ggtggtcagc tgtgggcatt gctatccgtg
    53521 ctctataggt aagccgaacg tttgtacgac acttgctgta ttaggtgtgc acgctgacgg
    53581 tggtttcagt gaatatgccg tggttccggc aaaaaatgcg tggaaaattc ctgaagcagt
    53641 ggccgatcaa tatgcggtga tgatcgaacc ttttaccatt gcggctaacg ttaccggtca
    53701 tggtcaaccg actgaaaatg ataccgttct ggtttacggt gccggtccaa tcggcctgac
    53761 gatcgttcag gtattaaaag gcgtctataa cgttaaaaat gtgattgttg ccgatcgcat
    53821 tgatgaacga ctggaaaaag cgaaagagag cggggcagac tgggcgatta ataacagcca
    53881 gacaccactt ggcgagattt tcgatgaaaa aggcatccaa ccgacattag ttatcgatgc
    53941 cgcttgtcat ccttcaatct tgaaagaggc cgtaacgctg gcttctccag cggcacgtat
    54001 tgtattgatg ggcttctcca gtgaaccgtc tgaagtgatt cagcaaggaa ttaccggaaa
    54061 agaactctct attttctctt cacgcttaaa tgcaaataaa ttcccggtcg ttatcgactg
    54121 gttaagtaaa gggttaatta aaccagaaaa actaattacc catacgtttg atttccagca
    54181 tgttgcggat gccattagtt tatttgaaca ggatcaaaaa cattgctgca aagtcttact
    54241 cactttttct gaataatacc aataacggcg aataagtagt acgcatctta cctctttttt
    54301 agagataacc attatgacaa tagaaaaaca cgaaagaagc actaaggatt tggtgaaagc
    54361 agcagtatcg ggatggctgg gcactgcgct tgaatttatg gatttccagt tatattcgct
    54421 cggcgcagcg ttagtgtttc atgaaatatt ttttcctgaa tcatcaacgg caatggcgtt
    54481 aattctggca atgggaacct atggtgcagg ttatgtggca cgtattgtcg gagcatttat
    54541 tttcggcaaa atgggcgaca gaatagggcg taaaaaagtg ctctttatta ccatcaccat
    54601 gatggggatc tgtaccacct taattggtgt gttaccgact tatgcacaaa ttggtgtttt
    54661 tgcccccatc ttgctggtga cgctgcgtat tattcagggg ttgggtgcag gtgcggaaat
    54721 ttccggtgcc ggtacgatgc tggcggaata tgcgccaaaa ggtaagcgcg gaattatctc
    54781 ctcatttgtg gctatgggaa ctaactgcgg aaccttgagc gcaacggcaa tctgggcctt
    54841 tatgttcttc attctcagta aagaggaact gctggcgtgg ggatggcgta tacctttcct
    54901 ggcgagcgtt gtcgtgatgg tctttgctat ctggttgcgt atgaatctga aagaaagccc
    54961 ggtctttgag aaggttaacg acagcaacca accgacagca aaacctgcac ctgctggtag
    55021 catgttccag agcaaatcct tctggctggc aacagggctg cgttttggtc aggctggtaa
    55081 ctcaggttta attcagactt tccttgcagg ctatttagtg cagacgttat tgtttaacaa
    55141 agcaattcca acagatgcat tgatgatcag ttcgattctc ggctttatga ccattccgtt
    55201 ccttggttgg ttatccgata aaattggtcg ccggatcccg tatattatta tgaatacctc
    55261 tgcgattgtg ctggcatggc caatgctttc tatcatcgta gataaaagct atgccccgag
    55321 caccattatg gttgcactga ttgtgattca taactgtgcg gtgcttggat tatttgctct
    55381 ggaaaacatt accatggcag aaatgttcgg ctgtaaaaac cgctttaccc gaatggctat
    55441 ttctaaagaa attggtggtc ttatcgcttc cggttttggt cctatcctgg cggggatttt
    55501 ctgcaccatg acggaatcct ggtatccgat cgccattatg atcatggcat attctgtgat
    55561 tggtttaatc tctgcgctga aaatgccaga agtgaaagac cgtgatttaa gtgcgctgca
    55621 agacgccgca gaagatcaac cacatgttgt aagagctgcg caaccgtcca gaagtctgta
    55681 aatcctcaat cccttctctt accggagagg gg
//
LOCUS       DAOFSV010000013        54266 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.013, whole genome shotgun sequence.
ACCESSION   DAOFSV010000013 DAOFSV010000000
VERSION     DAOFSV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.013"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1359)
                     /gene="rhlE"
                     /locus_tag="QBH27_000886"
     CDS             complement(<1..1359)
                     /gene="rhlE"
                     /locus_tag="QBH27_000886"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDQ7013239.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAILGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            1588..2259
                     /gene="cecR"
                     /locus_tag="QBH27_000887"
     CDS             1588..2259
                     /gene="cecR"
                     /locus_tag="QBH27_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDQ7013240.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2262..3257
                     /gene="hlyD"
                     /locus_tag="QBH27_000888"
     CDS             2262..3257
                     /gene="hlyD"
                     /locus_tag="QBH27_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDQ7013241.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3250..4986
                     /locus_tag="QBH27_000889"
     CDS             3250..4986
                     /locus_tag="QBH27_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013242.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            4979..6112
                     /locus_tag="QBH27_000890"
     CDS             4979..6112
                     /locus_tag="QBH27_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013243.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANAIAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6123..7229
                     /locus_tag="QBH27_000891"
     CDS             6123..7229
                     /locus_tag="QBH27_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013244.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7191..7601)
                     /locus_tag="QBH27_000892"
     CDS             complement(7191..7601)
                     /locus_tag="QBH27_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDQ7013245.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7734..8495
                     /locus_tag="QBH27_000893"
     CDS             7734..8495
                     /locus_tag="QBH27_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDQ7013246.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGESVLVAGDFNDWRQKANHPLKVLAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8492..9733
                     /gene="clsB"
                     /locus_tag="QBH27_000894"
     CDS             8492..9733
                     /gene="clsB"
                     /locus_tag="QBH27_000894"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDQ7013247.1"
                     /translation="MKCSWREGNMIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSTARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9733..10689
                     /locus_tag="QBH27_000895"
     CDS             9733..10689
                     /locus_tag="QBH27_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDQ7013248.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10725..11438)
                     /locus_tag="QBH27_000896"
     CDS             complement(10725..11438)
                     /locus_tag="QBH27_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDQ7013249.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11644..12348)
                     /locus_tag="QBH27_000897"
     CDS             complement(11644..12348)
                     /locus_tag="QBH27_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDQ7013250.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12485..12937)
                     /gene="moaE"
                     /locus_tag="QBH27_000898"
     CDS             complement(12485..12937)
                     /gene="moaE"
                     /locus_tag="QBH27_000898"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDQ7013251.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(12939..13184)
                     /gene="moaD"
                     /locus_tag="QBH27_000899"
     CDS             complement(12939..13184)
                     /gene="moaD"
                     /locus_tag="QBH27_000899"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDQ7013252.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(13177..13662)
                     /gene="moaC"
                     /locus_tag="QBH27_000900"
     CDS             complement(13177..13662)
                     /gene="moaC"
                     /locus_tag="QBH27_000900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDQ7013253.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13665..14177)
                     /gene="moaB"
                     /locus_tag="QBH27_000901"
     CDS             complement(13665..14177)
                     /gene="moaB"
                     /locus_tag="QBH27_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDQ7013254.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14199..15188)
                     /gene="moaA"
                     /locus_tag="QBH27_000902"
     CDS             complement(14199..15188)
                     /gene="moaA"
                     /locus_tag="QBH27_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDQ7013255.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLENDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15176..15320)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15585..16493
                     /gene="yvcK"
                     /locus_tag="QBH27_000903"
     CDS             15585..16493
                     /gene="yvcK"
                     /locus_tag="QBH27_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDQ7013256.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16531..18552)
                     /gene="uvrB"
                     /locus_tag="QBH27_000904"
     CDS             complement(16531..18552)
                     /gene="uvrB"
                     /locus_tag="QBH27_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDQ7013257.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19131..19808)
                     /gene="bioD"
                     /locus_tag="QBH27_000905"
     CDS             complement(19131..19808)
                     /gene="bioD"
                     /locus_tag="QBH27_000905"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDQ7013258.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(19801..20556)
                     /gene="bioC"
                     /locus_tag="QBH27_000906"
     CDS             complement(19801..20556)
                     /gene="bioC"
                     /locus_tag="QBH27_000906"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDQ7013259.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(20543..21697)
                     /gene="bioF"
                     /locus_tag="QBH27_000907"
     CDS             complement(20543..21697)
                     /gene="bioF"
                     /locus_tag="QBH27_000907"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDQ7013260.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVVDDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21694..22734)
                     /gene="bioB"
                     /locus_tag="QBH27_000908"
     CDS             complement(21694..22734)
                     /gene="bioB"
                     /locus_tag="QBH27_000908"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDQ7013261.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            22821..24110
                     /gene="bioA"
                     /locus_tag="QBH27_000909"
     CDS             22821..24110
                     /gene="bioA"
                     /locus_tag="QBH27_000909"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDQ7013262.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETSRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24169..24645
                     /locus_tag="QBH27_000910"
     CDS             24169..24645
                     /locus_tag="QBH27_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDQ7013263.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            24797..26080
                     /locus_tag="QBH27_000911"
     CDS             24797..26080
                     /locus_tag="QBH27_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDQ7013264.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26221..28482)
                     /locus_tag="QBH27_000912"
     CDS             complement(26221..28482)
                     /locus_tag="QBH27_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDQ7013265.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMAEVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28665..30098)
                     /locus_tag="QBH27_000913"
     CDS             complement(28665..30098)
                     /locus_tag="QBH27_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDQ7013266.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30174..31226)
                     /locus_tag="QBH27_000914"
     CDS             complement(30174..31226)
                     /locus_tag="QBH27_000914"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDQ7013267.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            31410..32363
                     /locus_tag="QBH27_000915"
     CDS             31410..32363
                     /locus_tag="QBH27_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7013268.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFSIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32404..33399)
                     /gene="pgl"
                     /locus_tag="QBH27_000916"
     CDS             complement(32404..33399)
                     /gene="pgl"
                     /locus_tag="QBH27_000916"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDQ7013269.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33554..34372
                     /gene="ybhA"
                     /locus_tag="QBH27_000917"
     CDS             33554..34372
                     /gene="ybhA"
                     /locus_tag="QBH27_000917"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDQ7013270.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34373..35431)
                     /gene="modC"
                     /locus_tag="QBH27_000918"
     CDS             complement(34373..35431)
                     /gene="modC"
                     /locus_tag="QBH27_000918"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDQ7013271.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGCKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35434..36123)
                     /gene="modB"
                     /locus_tag="QBH27_000919"
     CDS             complement(35434..36123)
                     /gene="modB"
                     /locus_tag="QBH27_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDQ7013272.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(36123..36896)
                     /gene="modA"
                     /locus_tag="QBH27_000920"
     CDS             complement(36123..36896)
                     /gene="modA"
                     /locus_tag="QBH27_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7013273.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37063..37212)
                     /gene="acrZ"
                     /locus_tag="QBH27_000921"
     CDS             complement(37063..37212)
                     /gene="acrZ"
                     /locus_tag="QBH27_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDQ7013274.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37341..38129
                     /gene="modE"
                     /locus_tag="QBH27_000922"
     CDS             37341..38129
                     /gene="modE"
                     /locus_tag="QBH27_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDQ7013275.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38197..39669
                     /gene="modF"
                     /locus_tag="QBH27_000923"
     CDS             38197..39669
                     /gene="modF"
                     /locus_tag="QBH27_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDQ7013276.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTSRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQHLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            39932..40948
                     /gene="galE"
                     /locus_tag="QBH27_000924"
     CDS             39932..40948
                     /gene="galE"
                     /locus_tag="QBH27_000924"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDQ7013277.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRRAGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            40958..42004
                     /gene="galT"
                     /locus_tag="QBH27_000925"
     CDS             40958..42004
                     /gene="galT"
                     /locus_tag="QBH27_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDQ7013278.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42008..43156
                     /gene="galK"
                     /locus_tag="QBH27_000926"
     CDS             42008..43156
                     /gene="galK"
                     /locus_tag="QBH27_000926"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDQ7013279.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43150..44190
                     /gene="galM"
                     /locus_tag="QBH27_000927"
     CDS             43150..44190
                     /gene="galM"
                     /locus_tag="QBH27_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDQ7013280.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            44393..45145
                     /gene="gpmA"
                     /locus_tag="QBH27_000928"
     CDS             44393..45145
                     /gene="gpmA"
                     /locus_tag="QBH27_000928"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDQ7013281.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45312..46364)
                     /gene="aroG"
                     /locus_tag="QBH27_000929"
     CDS             complement(45312..46364)
                     /gene="aroG"
                     /locus_tag="QBH27_000929"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDQ7013282.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            46680..47060
                     /locus_tag="QBH27_000930"
     CDS             46680..47060
                     /locus_tag="QBH27_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDQ7013283.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47174..48115
                     /gene="zitB"
                     /locus_tag="QBH27_000931"
     CDS             47174..48115
                     /gene="zitB"
                     /locus_tag="QBH27_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDQ7013284.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNRGVSGHSHHHH"
     gene            complement(48112..48831)
                     /gene="pnuC"
                     /locus_tag="QBH27_000932"
     CDS             complement(48112..48831)
                     /gene="pnuC"
                     /locus_tag="QBH27_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDQ7013285.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWIKSARERGSRALSH"
     gene            complement(48869..49912)
                     /gene="nadA"
                     /locus_tag="QBH27_000933"
     CDS             complement(48869..49912)
                     /gene="nadA"
                     /locus_tag="QBH27_000933"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDQ7013286.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKANYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(50190..50265)
                     /locus_tag="QBH27_000934"
     tRNA            complement(50190..50265)
                     /locus_tag="QBH27_000934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50230..50232),aa:Lys,seq:ttt)
     gene            complement(50314..50389)
                     /locus_tag="QBH27_000935"
     tRNA            complement(50314..50389)
                     /locus_tag="QBH27_000935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50354..50356),aa:Lys,seq:ttt)
     gene            complement(50393..50468)
                     /locus_tag="QBH27_000936"
     tRNA            complement(50393..50468)
                     /locus_tag="QBH27_000936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50433..50435),aa:Val,seq:tac)
     gene            complement(50520..50595)
                     /locus_tag="QBH27_000937"
     tRNA            complement(50520..50595)
                     /locus_tag="QBH27_000937"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50560..50562),aa:Lys,seq:ttt)
     gene            complement(50598..50673)
                     /locus_tag="QBH27_000938"
     tRNA            complement(50598..50673)
                     /locus_tag="QBH27_000938"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50638..50640),aa:Val,seq:tac)
     gene            complement(50709..50784)
                     /locus_tag="QBH27_000939"
     tRNA            complement(50709..50784)
                     /locus_tag="QBH27_000939"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50749..50751),aa:Lys,seq:ttt)
     gene            complement(50949..51740)
                     /gene="cpoB"
                     /locus_tag="QBH27_000940"
     CDS             complement(50949..51740)
                     /gene="cpoB"
                     /locus_tag="QBH27_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDQ7013287.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVVASTTPTADAGTSNAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(51750..52271)
                     /gene="pal"
                     /locus_tag="QBH27_000941"
     CDS             complement(51750..52271)
                     /gene="pal"
                     /locus_tag="QBH27_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDQ7013288.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(52306..53598)
                     /gene="tolB"
                     /locus_tag="QBH27_000942"
     CDS             complement(52306..53598)
                     /gene="tolB"
                     /locus_tag="QBH27_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDQ7013289.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(53731..>54266)
                     /gene="tolA"
                     /locus_tag="QBH27_000943"
     CDS             complement(53731..>54266)
                     /gene="tolA"
                     /locus_tag="QBH27_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDQ7013290.1"
                     /translation="ADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAA
                     KAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKS
                     AIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPP
                     SQAVYEVFKNAPLDFKP"
BASE COUNT        13119 a        14407 c        13756 g        12984 t
ORIGIN      
        1 ctgcgcagcg gcaggtttac gcggacggcg gcgacgttgt tgttcgcctg ccggtttggc
       61 atcgccgaga cgacgagacg gtttttctgc gggcttcgcg ttaccagact tcgcaccact
      121 ttccgcacgg cgtggttgct gttgaccgcg accaccgccc tgtccacgac cgccgccacg
      181 ttgctggcga ccgttttgga tcggttcggc tttgattgac gggtccggct catagcccag
      241 aatcgcaatg cgcgggatct cttttttcag cagtttttcg atatcacgca gcagtttgtg
      301 ttcatcaaca cacaccagcg acaatgcttc gccagttgcc gccgcacggc cagtacgccc
      361 gatacggtga acataatctt ccggtacgtt tggtaactca tagttgacga cgtgcggcaa
      421 ctcttcgata tccagaccac gcgcagcgat gtcagttgcc accagtacac gaatatcgcc
      481 cgatttaaaa tcggccagcg cacgagtacg cgcaccttgc gatttattac cgtggatcgc
      541 cgcactacgg atgccatctt tattaagctg ttcagccaga tggttagcgc catgtttggt
      601 acgggtaaac accagcacct gctgccagtt ccctttccca atcatatgcg acagcaattc
      661 gcgtttgcgt ttcttatcga caaagtgaac gtgctgggtc acctgatcag acgcagtatt
      721 gcggcgtgcc acttcgattt ccagcgggtt gtgcaacagt ttttccgcca gggctttaat
      781 atcgtcagag aaggtcgcgg agaacaacag gttctggcgc ttcgctggca gttttgttaa
      841 cacgcgacgg atatcgtgga taaagcccat gtcgagcatg cggtcagctt catcaaggac
      901 gaggatttca acctgatcca gcttcactgc attttgatgt tccaggtcca gcagacgtcc
      961 cggcgttgcc accagcacat caacgccgcc gcgcagcttc atcatctgcg ggttaatgct
     1021 gacaccgcca aacaccacca gcgaacgaat gttcaggtat ttgctgtaat cacggacgtt
     1081 ttcgccaatc tgcgccgcca gttcacgggt cggggtaaga atgagcgcac gcaccggacg
     1141 acgccctttg gcgtgcggct ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc
     1201 cgctgtttta cctgtaccgg tctgggcgct ggccatcagg tcgcggcctt ccagcaccgc
     1261 agggatcgcc tgctgctgaa taggggtggg ttcacggtaa ccctgctcgg caacggcgcg
     1321 caggatatca gggcttaaac ccaaagaatc gaaagacata actactccga accgccccga
     1381 ccgttaccgg tgtagttttc agggagatac tgaaaaggaa aatgacaaaa accacaatgt
     1441 catgagggag cggagtgtag cagtttttgt gacgcagcgc ataaattatc cctgtgactg
     1501 gcgtagctaa aattcttagc catactggac aactccccta tggagtcata atcttaatca
     1561 atcatttgat taagaatgga ctgtgcgatg aataatcctg ccatgacaat caagggtgaa
     1621 caggcgaaaa aacagctgat tgctgcggca ctggcacagt ttggtgaata tggaatgaac
     1681 gccaccaccc gcgagatagc cgcccaggcc gggcagaata tcgctgccat cacctactac
     1741 ttcggttcga aagaagattt gtacctcgcc tgcgcccagt ggattgctga ttttattggc
     1801 gagcagttcc gtccgcacgc cgaagaagcc gaacgtttgt tcgcgcaacc acagccggat
     1861 cgggctgcca tccgtgaact gatccttcgc gcctgcagaa acatgattaa gctgctcacc
     1921 caggatgaca ccgtcaacct cagcaaattt atctcccgtg agcagctctc tcccacggca
     1981 gcctaccacc tggtgcacga acaggtgatt agtccgctac acagtcacct tacacgcctg
     2041 attgccgcct ggaccggctg cgacgctagc gatacccgca tgatccttca tacccatgcg
     2101 ttgattggtg agattctggc gtttcgtctt ggcaaagaaa ctattctgtt acgtaccggc
     2161 tggaccgcgt tcgatgaaga aaagaccgaa ctgatcaacc agacggtgac ctgtcatatt
     2221 gacctgattt tgcaaggatt atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga
     2281 tcggactggc ggtagtagta cttgccgccg tggttgccgg aggctactgg tggtatcaaa
     2341 gccgccagga taacggcctg acgctgtatg gcaacgtgga tattcgtacg gtgaatctta
     2401 gtttccgtgt tggggggcgc gttgagtcgc tggcggtgga cgaaggtgat gcgatcaaag
     2461 cgggccaggt gctgggcgaa ctggatcaca agccgtatga gattgccctg atgcaggcga
     2521 aagcgggtgt ttcggtggct caggcgcagt atgacctgat gcttgccggg tatcgagatg
     2581 aagaaatcgc tcaggccgcc gcagcggtga aacaggcgca agctgcctat gactatgcgc
     2641 agaacttcta taaccgccag caaggactgt ggaaaagccg caccatttcg gcaaatgacc
     2701 tagaaaatgc ccgctcctcg cgcgaccagg cgcaggccac gctgaaatca gcgcaggata
     2761 aattgcgtca gtaccgttcc ggtaaccgcg aacaggacat cgcccaggcg aaagccagcc
     2821 tcgaacaagc acaggcgcaa ctggcgcagg cggagctgaa tttgcaggac tcaacgttga
     2881 tagcaccgtc tgatggcacg ctgttaacgc gcgctgtaga gccaggcacg gtgcttaatg
     2941 aaggtggcac ggtatttacc gtctcgctga cgcgtccggt gtgggtacgc gcctatgttg
     3001 atgaacgtaa tcttgaccag gcccagccgg ggcgcaaagt gttgctttat accgatggtc
     3061 gcccggacaa gccgtatcac gggcagattg gtttcgtttc gccgactgct gaatttaccc
     3121 cgaaaaccgt cgaaacaccg gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg
     3181 tgaccgacgc cgatgatgcg ttacgccagg gaatgccggt aacggtgcaa ttcggtgacg
     3241 aggcaggaca tgaatgatgc cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc
     3301 atggacaagc ccgccgtcgc accgctcgac tgcaccattc acgccggtta tgtgacgggg
     3361 ctggttgggc cggacggtgc gggcaaaacc acgctgatgc ggatgctggc gggattactg
     3421 aaacccgaca gcggcagtgc cacggtgatt ggctttgatc cgatcaaaaa cgacggcgcg
     3481 ctgcacgccg tgctcggcta tatgccgcag aaattcggcc tgtatgaaga tctgacggtg
     3541 atggagaacc tcaatctgta cgcggatttg cgcagcgtca ccggcgaggc acggaagcaa
     3601 acttttgctc gcctgctgga gtttacgtct cttggcccgt ttaccggacg cctggcgggc
     3661 aagctctccg gtgggatgaa acaaaaactt ggcctggcct gtaccctggt gggcgaaccg
     3721 aaagtgttgc tgctcgatga acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg
     3781 tggcagatgg tgcatgagct ggcgggcgaa gggatgttaa tcctctggag tacctcgtat
     3841 ctcgacgaag ccgagcagtg ccgtgacgtg ttactgatga acgaaggcga gctgctgtat
     3901 cagggagaac cgacggccct gactcaaacc atggccggac gcagctttct gatgaccagc
     3961 ccgcacgagg gcaaccgcaa actgttgcaa cgggcattga aactgccgca ggtcagcgac
     4021 ggcatgattc aggggaaatc ggtacgtctg atcctcaaaa aagagaccac accagacgat
     4081 attcgccatg ccgacgggat gccggaaatc aacattaacg aaactacgcc acgttttgaa
     4141 gatgcgttta ttgatttgct gggcggtgcc ggaacctcgg aatcgccgct gggcgcaata
     4201 ttgcatacgg tggaaggtac acctggcgag acggtgatcg aagcgaaaga gctgaccaaa
     4261 aagtttggcg attttgccgc caccgatcac gtcaactttg ccgtcaaacg cggtgaaatc
     4321 tttggtttgc tcgggccaaa cggcgcaggg aaatcgacca cctttaagat gatgtgcggt
     4381 ttgctggtac cgacctctgg tcaggcgctg gtgctgggca tggatctgaa agagagttcc
     4441 ggtaaagcgc gccagcatct cggctatatg gcgcaaaaat tttcgctcta cggcaacctg
     4501 acggtcgaac agaatttacg ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag
     4561 aacgaaaaaa tctcccgtat gagcgaggcg ttcggcctga aaagtatcgc ctcccacgcg
     4621 accgatgaac tgccattagg ttttaaacag cggctggcgc tggcctgttc gctgatgcat
     4681 gaaccggaca ttctgtttct cgacgaaccg acgtccggcg ttgatcccct cacccgccgt
     4741 gaattttggc tacatatcaa cagtatggta gagaaaggcg tcacggtgat ggtcaccacc
     4801 cactttatgg atgaagcgga atattgcgac cgcatcggcc tggtgtaccg cgggaaatta
     4861 atcgccagcg gcacaccgga cgatttgaaa gcgcagtcgg ccaacgatga gcaacccgat
     4921 cccacgatgg agcaagcctt tattcagttg atccacgact gggataagga gcatagcaat
     4981 gagtaatccg atcctgtcct ggcgtcgcgt gcgggcgctg tgcgtcaaag agacgcggca
     5041 gatcgttcgc gatccgagta gctggctgat tgcagtagtg atcccactgc tactgctgtt
     5101 tatttttgga tacggcatta acctcgactc cagcaagctg cgcgtcggga ttttactgga
     5161 acaacgtagc gaagcggcgc tggatttcac ccacaccatg accggttcgc cctacatcga
     5221 cgccaccatc agcgataacc gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg
     5281 cggtctggtg gttattccgg tggatttcgc cgaacagatg gagcgcgcca acgccatcgc
     5341 accgattcag gtgattaccg acggcagtga gccgaatacc gctaactttg tgcaggggta
     5401 tgtcgaaggg atctggcaga tctggcaaat gcagcgagcg gaggataacg ggcagacttt
     5461 tgaaccgctt attgatgtac aaacccgcta ctggtttaac ccggcggcga taagccagca
     5521 ctttattatc cccggtgcgg tgactattat catgacggtc atcggtgcga ttctcacctc
     5581 gctggtggtg gcgcgagaat gggaacgcgg caccatggag gctttgctct ctacggagat
     5641 tacccgcacg gagctgctgc tgtgtaagct gatcccttat tactttctcg ggatgctggc
     5701 gatgttgctg tgtatgctgg tgtcggtgtt tattctcggc gtgccgtatc gcgggtcgct
     5761 gctgattctg ttttttatct ccagcctgtt tttactcagt accctgggga tggggctgct
     5821 gatttccacg attacccgca accagttcaa cgccgctcag gtggccctga acgcggcttt
     5881 tctgccgtcg attatgcttt ccggctttat atttcagata gacagtatgc ccgcggtgat
     5941 ccgcgcggtg acgtacatta ttcccgcacg ttatttcgtc agcactctgc aaagcctgtt
     6001 cctcgccggg aatattccag tggtgctgat agtgaacgtg ctgtttttga tcgcttcggc
     6061 ggtgatgttt atcggcctga cgtggctgaa aaccaaacgt cggctggatt agggagaaga
     6121 gcatgtttca tcgcttatgg acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac
     6181 cgcaaacccg cgcaattctg attttacccg tgctgattca ggtgatcctg ttcccgttcg
     6241 ccgccacgct ggaagtgacc aacgccacca tcgccatcta cgatgaagat aacggcgagc
     6301 actcagtaga acttactcaa cgttttgccc gcgccagcgc ctttacccat gtgctgctgc
     6361 tgaaaagccc gcaggagatc cgcccgacca tcgacacaca aaaggcgtta ctactggtgc
     6421 gtttcccggc tgacttctcg cgcaaactgg ataccttcca gaccgcgccg ttgcagttga
     6481 tcctcgacgg gcgtaactcc aacagtgcgc aaattgccgc caactacctg caacagatcg
     6541 tcaaaaatta tcagcaggag ctgctggaag gaaaaccgaa acccaacaac agcgagctgg
     6601 tggtacgcaa ctggtataac ccgaatctcg actacaaatg gtttgtggtg ccgtcgctga
     6661 tcgccatgat caccactatc ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac
     6721 gtgaacaagg tacgctcgat cagttgctgg tttcgcccct caccacctgg cagatattca
     6781 tcggcaaagc cgtaccggca ttaattgtcg ccaccttcca ggccaccatt gtgctggcga
     6841 ttggtatctg ggcgtatcaa atccccttcg ccggatcgct ggcgctgttc tactttacga
     6901 tggtgattta cggtttatcg ctggtgggat tcggtctgtt gatttcatca ctctgttcaa
     6961 cacaacagca ggcgtttatc ggcgtgtttg tctttatgat gcccgccatt ctcctttccg
     7021 gttacgtttc gccggtggaa aacatgccag tgtggctgca aaatctgacg tggattaacc
     7081 ctattcgcca ctttacggac attaccaagc agatttattt gaaggatgca agtctggata
     7141 ttgtgtggaa tagtttgtgg ccgctactgg tgataacggc cacgacaggg tcagcggcgt
     7201 acgcgatgtt tagacgtaag gtgatgtaac ttcttatctt tcgccagcaa agacaccacc
     7261 gccgggccgg caaggattgc cagccctgca attgccagca aaaagttgtt ttccagtacg
     7321 cgggacaaca gccacagcac aatcagcgct gcaccaaaat accaggtcgt ggtcagctct
     7381 tccagcacgt ctgccacctc acgccagcgt tgctctgggt ggcgctgaaa caccagcatc
     7441 atcactaccg tgacacaata cagtgcgcat aatcctacgc cgacgtgaac cagagtttta
     7501 ctcatccaac ccaccaccag cagcgccact accagtaaat gcaacataat ctgccagcaa
     7561 cttaggccag ttgcgacacg aacacgttgt tgccacttca tggcttctct cctgaagaat
     7621 ttatctctac ttattcagag taccgaactt tacggaaaat tccgtaagac cgcacctttt
     7681 tgtgacgccg actacactat ttcaatgaga taagaggcaa agacaggaaa attatgcccg
     7741 atcaaacaca acaattttcg ttcaaggtgc tcaccatcaa tattcacaaa ggctttaccg
     7801 cgtttaaccg acgcttcatt ttaccggaac ttcgcgacgc cgtgcgtacc gtcagcgccg
     7861 atattgtttg cctgcaggaa gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa
     7921 actggcccga tacctcgcac tacgagtttc tcgccgacac catgtggagc gatttcgcct
     7981 acggtcgcaa tgccgtatac ccggaaggac atcacggtaa cgccgttctt tcacgttatc
     8041 ccattgaaca ttatgagaat cgtgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc
     8101 tctactgccg cattgtgccg ccgatgaccg ggaaagcgat tcatgtgatg tgcgtacatc
     8161 tgggcctacg tgaggcgcac cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg
     8221 agctaccgga cggcgaatcg gtattggtag cgggtgattt caacgactgg cggcaaaaag
     8281 ctaatcatcc gttaaaagtg ctggccgggc tggatgagat ttttacccgc gcccacggac
     8341 gtccggcgcg cacgtttccg gtgcaatttc ctctactacg actggacagg atctacgtca
     8401 aaaatgccag cgccagcgcg ccaaccgcgt tgccgctgcg gacatggcga cacctttctg
     8461 atcatgcccc tttaagtgcg gagattcatt tatgaaatgt agctggcgcg aaggcaatat
     8521 gatccagttg ctggaaaacg gcgagcaata ttatcccgcg gtgtttaagg cgattggcga
     8581 ggcgcaagaa cgcatcattc ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca
     8641 gctgcatgcg gcgctattgg cagcagcgca acgcggggta aaagcggaag tcttgctgga
     8701 tggctacggt tcgccggatc tcagcgatga atttgtcaat gaactgacgg cagctggcgt
     8761 ggtgttccgc tactacgatc cccgccctcg cctttttggt atgcgcacca atgtgtttcg
     8821 ccggatgcat cgcaaaattg tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa
     8881 ttactccgcc gagcatatgt ccagctacgg cccggaggcc aaacaggatt acgcggtacg
     8941 cctcgaaggg ccgattgtcg aagatattct ccagtttgag ctggaaaacc tgccaggaca
     9001 aagcacggcg cgacgctggt ggcgacgcca tcataaagcg gaagagaacc gccagccggg
     9061 agaagcgcag gtattgctgg tctggcgcga taacgaagaa catcgcgatg atatcgaacg
     9121 tcactatctg aaaatgctca ctcaggcgcg gcgggaagtg attatcgcca acgcctactt
     9181 cttccccggc tatcgttttt tacacgcctt gcgtaaagcg gcacgacgcg gggtgcggat
     9241 caaactgatc attcagggcg aaccggatat gccgattgtc agagtcggcg cgcgtttgct
     9301 gtataactat ctggttaaag gtggcgttca ggtgtttgag taccgccgcc gcccgctcca
     9361 cggcaaagtg gcattgatgg acgatcactg ggcgacagta gggtccagta atctcgatcc
     9421 gctcagtttg tcactgaatc tcgaagcaaa tgtcatcatc cacgatcgta attttaacca
     9481 gacgctgcgc gataatctga acggcattat cgccgcagat tgtcagcagg tcgatgagac
     9541 catgctgccg aaacgcacct ggtggaacct gactaaaagc gtgctggcgt tccactttct
     9601 acgccacttc ccggcgctgg tgggctggct tccggcgcac acgccacgtc tggcgcaggt
     9661 tgatcccccc gcacaaccga caatggaaac gcaggatcgg gtagaaactg aaaacacggg
     9721 ggtaaaaccc tgatgagtaa atcacacccg cgctggcgct tagcaaagaa gatcctcacc
     9781 tggctgtttt ttatcgcggt gattgtgttg ttggtggtct acgccaaaaa agtggactgg
     9841 gaagaggtct ggaaggtcat ccgagactac aatcgcgttg cactgctcag tgcggtcggg
     9901 ctggtggtcg tcagctatct gatttacggt tgctacgacc tgctcgcccg tttctactgc
     9961 ggtcacaaac tggcgaagcg ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac
    10021 ctgacactca gtacctgggt cggcggcatt ggtatgcgct atcgtttgta ctcccgactg
    10081 gggttaccgg gcagcactat tacgcggatt ttttcgctca gtattaccac caactggcta
    10141 ggctatattt tgctagcggg ggttatcttt accgcaggcg tggtggagtt gccggatcac
    10201 tggtatgtcg atcaaactac gctgcgtatt ctcggcattg gcttactgat gattatcgcg
    10261 gtttatttgt ggttttgcgc tttcgcgaag caccgccata tgaccatcaa agggcaaaag
    10321 ctggtgctgc cttcgtggaa attcgccctc gcccagatgc tgatttccag cgttaactgg
    10381 atggtaatgg gagcgattat ctggctgtta cttggtcaaa gcgtaagcta tttctttgta
    10441 ctgggcgtgt tgctggttag tagtattgct ggcgtcatcg tgcatattcc ggcgggaatt
    10501 ggtgtgctgg aagcggtgtt tatcgcgcta ctggctgggg agcatacctc caaaggcaca
    10561 attatcgccg ccctacttgc ttaccgtgtg ctgtattact ttatcccact gctgctggcg
    10621 ctgatctgtt acctgttgct ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg
    10681 gcgatgtgaa gtatagttaa gttctttagt atgtgcattt acggctaatg aaaaaaccgc
    10741 gtatgccttt gccagacaag cgttatagct atagcagcaa gaatcccaaa gaaaccgata
    10801 aaatagtaat agagtgttaa tgcacctata aaagcacaac gaaccggact ccataattct
    10861 tcactatggc tttttcctgt cagttcaagg agcttatttc gtccatgcga gataatgcct
    10921 gaccataaga caatcattaa gcaacttatt acccaaatgg gtcgttcact cattaatgtg
    10981 gcattcacga tgattaccag ggccaaccct gtcaacgtca tcataatgat aaaacgatga
    11041 gaaccaggat caaatttaaa caatagacct gccagcatac tgattaaaaa catcgatccc
    11101 gcagtgctga acacgacgat aatggcagat acaggcgcta aactcgcaac gacaatcgcc
    11161 accaaagaga ttaaaaacaa atatattacg cccgcgatgg cggtatttat cttgttgata
    11221 tctttactta agccatacat agaaagtaat aataatccta tagcaacata catttgcggt
    11281 gaatcatatt ttgttgccgt aatttcgaga tacttagcgc cccaggccag ccctgcggtt
    11341 gccaccaaac ccaaagcggt aatgagccat atgccgttta atatcctggc ttcgtgctga
    11401 aaatctaatt tattgctatt ttgcgagtat gattccattc ttttagttgt ccttagaaaa
    11461 aacattaata ttcaccatca ataaatttaa aaccacgcat acaaaatact tttgcattta
    11521 tggatacaga aaacgcttcc gctcgtccat aaggcatatt ttatacctgg tacttgttaa
    11581 gtgtcgtttt taataagaaa aaaaatcccg acacatggcc gggatattga tgatgaaaaa
    11641 gaattaacgg cggttgccga agatccgcag caacatcagg aacaggttga tgaagtccag
    11701 atacaaggtt aacgcgccaa gaatggaata tttgcgcagg ttcgacgtgt cgcgagtatc
    11761 aatctgctca cccatatttt tcagtttctg ggtgtcatac gccgtcaatc cgacaaagac
    11821 aatcacgccg atgtaggtga ctgcccacat caatgcttcg cttttcagcc agaagttaac
    11881 cagcgatgcc agcacaatgc cgattaacgc cataaacagc atattgccaa agccgcttag
    11941 atcgcgcttc gtggtgtaac cgtacaggct cattgcgccg aacatcccgg cagtaacgac
    12001 gaaagtactg gcgatagaag cagcggtata gacaatgaaa atactggaaa gcgtaagacc
    12061 cgtcagcgcc gaataaagca taaagagcat cgtcgttaca cctgcgctca gcttttgaat
    12121 catcgctgat aacacaataa ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac
    12181 acggttagta aacaacagct ccatcacggc cgcggaatta gccgcatacc aggcaacaaa
    12241 tgctgtcagc aacaagccaa cggtcatcca gccatagact tgcgccatat aagtttgcaa
    12301 gccagcccgg ggttgtacga ttgaatcaga acgtgggaat ctgtccatga ctttctcctg
    12361 aagatataag gaatatctta aggatactgc aaaatgatga ggctgtgcat cgacgcagcg
    12421 taaacggatg tactgagcgg tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa
    12481 taaactacca gcgttttgcc gcctgctgat cgctctcccg agcttcgacc cagcggtcgc
    12541 cttccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa
    12601 actgcccggc ttcaaacgca ctgctgcgat gcgcactggt aacaccgaca aaaacaattt
    12661 catcgcccgg ccataattca ccaatgcggt gaattaccgt gacgcgcccc agcggccagc
    12721 ggttacgcgc ttcatcaacg atttctgcca gtgctttttc tgtcattccc ggatagtgtt
    12781 cgagggttaa tgctttgacg ctgtcgccca ggttatggtt gcgcacctta ccggtaaagg
    12841 tgactaccgc accgtcttcg tcacgctcag ccagccacgg gtactcttct cctacgctga
    12901 acggctgcgg gccgacaaca attttggttt ctgccatctt aacctccggt taccggcggg
    12961 aagaaagcca cttcgtcgcc gtcagtcagc gaatggtcaa aactcaccag cgtctggttg
    13021 acagcagcca gcaatttgcc atcttccagc gccagagccc agcgatcgct ctgcgcagcc
    13081 agatgctggc gtaacgcttc aacggttggg aaatccgcag ccacttcggt tgcatcagtt
    13141 cccaccaact cgcgcacctg ggcgaaaaaa agaacgttaa tcatccgctt ccaccttaaa
    13201 gtcacccgac ttgccgccgc tctttgccag caaacgtacc ggaccaatca ccatatcttt
    13261 ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaacgc
    13321 ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac gtacccgatt
    13381 gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg ggtgacacag
    13441 cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcgatacgcg cagtggcaaa
    13501 tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt
    13561 gacaaaggct tcggcgcgcg cttcacgcac ggtttccgct ttggcggaga catcgaccat
    13621 gtgcgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt
    13681 ggatggaaat tacacggacg cgtacgggca tccagctgcg gcgcaatgat attttcccat
    13741 gcggtacggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttagcgacg
    13801 cccgctaccg cacgagattg caacgtggaa gtgccaatct cttcgaacga caacatccgg
    13861 aacacttcac caaaaccttc aacttcacgg tcgaacaacg gcagcagcgc ttcgggagcc
    13921 tgatctcctt ccgtcaggcc agtaccaccc gtaatcaaca ccacttgtac atcgtcgctg
    13981 gcgatccacg cagagacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta
    14041 tcgacaacgt gatggcccgc ttcttgcgcc gagtcgcgaa gatagtgacc ggaggtatcg
    14101 tcttcttcac cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca
    14161 gtgcttacct gactcatctg atctctcctt ttgacgtttt agccaccaat gtacgataag
    14221 ttttgcgtaa taccggtgtt attttgatgc aggaaatggg tctgtttttt ctcccgcagc
    14281 gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgttttccag cagatcgcgc
    14341 aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc
    14401 aggcggttgc aagtggcgca gaaatctttt tcatacggca tgataaggcc aatctctccg
    14461 gcgtaatccg gatgacaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg
    14521 atccagccgc gacgcagtaa ctcgtcacgc agaacctgac cagagatgtg atgcttacgg
    14581 aagagctcgc tgccctcgcc cgtttccatc agttcgatga aacgcagctg gatgggacga
    14641 tgctggatcc agttcagaaa ggtgtcgagc tggtgatgat tcacatcacg catcagcacg
    14701 gtattgactt tgaccttctc aaaaccggcc tcaaatgcag catcgattcc tgccatgacc
    14761 tgattgaatt tatcctgccc ggtaatggca tgaaactggc gagcgtcaag gctatcaacg
    14821 ctgacgttga tgccagtaag tccagcatcg cgccagttcg ccacatcgcg ctccagacgg
    14881 taaccattgg tggtgaccgc tatctggcga atagcgtcgt tttctcgcac agcggcgatg
    14941 atatcggtaa agtcgcggcg taaagatggc tcaccacctg tcagacggac tttttcggtg
    15001 cccagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtcag gaagccttta
    15061 ttggtgacgc cgctcggctt gtaaccatcc ggcaggcagt aggtgcaacg aaagttacac
    15121 acatcagtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt
    15181 gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg
    15241 gcaatcttta cgagcattgt cacggccaaa gcaccgtatc agttgaccca ggtgcagagg
    15301 ctagagtgtt tagtggttaa gccgatacta gcgtgtaaat gagattttta ccatccacac
    15361 tttcgctatg taatcatgta tatagcgtca tgatcgtgct atttcgctac agaataaggg
    15421 aaaatggcgg ttttactttg atatacatca tcagggaggc atagcgtgca tcgtccttta
    15481 gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttcctcgca
    15541 atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc
    15601 tggctgatct tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct
    15661 catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacc gataacggtg
    15721 gctcgacggg gcgtattcgc cgctcagaag gcggcattgc ctggggcgat atgcgcaact
    15781 gcctcaatca gctgataact gaacccagcg tcgcttccgc aatgtttgaa tatcgtttcg
    15841 gcggtaatgg cgaactttcc gggcacaacc ttggaaacct gatgttaaag gcactggatc
    15901 accttagcgt gcgacctctt gaagcgatca atctgattcg aaatctgctg aaagtggacg
    15961 cacatttgat tccgatgtcg gagcatcctg ttgatctgat ggcgattgac gatcaggggc
    16021 atgaagttta cggcgaggtc aatatcgacc agttaactgc gccgattcaa gagttattgt
    16081 taacgccgaa tgtacccgca acgcgtgagg cggttcacgc tatcagtgaa gcggatctca
    16141 tcattattgg gcctggcagt ttttacacca gcctgatgcc aattctgctg ctgaaggaaa
    16201 tcgcacaggc attacgccgc acgccagcgc cgatggtcta tatcggcaat ctggggcgtg
    16261 agttgagttt acctgcggct aatctgaagc tggaaagcaa gctggcaatt atggagcagt
    16321 atgttggtaa aaaggtaatt gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg
    16381 tgaaagagcg gattgtgatt caggaagtgc tggaggccag cgatattccg tatcgtcatg
    16441 accgccagtt gttgcatagc gcgctggaaa aggcattaca ggctttaggt taacacgaag
    16501 ccggaagaat attccggctt cgtcatcttg tcacgacgcg gcaataaaca gctcacgcaa
    16561 ctgatgcaac tggtcacgaa tttgcgccgc ttcttcgaac tccagattct gcgcgtgttg
    16621 catcatcaac ccttccagtt catggatctt ctgctgtaac gctttaggcg acatatccat
    16681 cggcacatta tccggctcaa caatcgggcg cgattttcct ctgcccttcg ctttggtttt
    16741 ggcaatgttc tgccccagcg ccaggatatc gaccactttc ttgttcaagc cttgcggcgt
    16801 aatgccgtgt tcctcgttgt acttctgctg tttctcgcgg cgacgttcgg tttcgccaat
    16861 cgctttcgcc attgatgggg tgatcttatc gccgtagaga atcgctttac cgttaacgtt
    16921 acgtgccgca cgaccaatgg tctggatcaa cgaacgttcg gaacgcagga agccttcttt
    16981 gtcagcgtcg aggatcgcca ccagcgaaac ctccggcata tccagacctt cgcgcagtaa
    17041 gttgatccct accaatacgt cgaactcacc cagacgcaag tcgcggataa tctccatacg
    17101 ttcgacggta tcgatatctg agtgaagata acgcacgcgc tcaccgtgtt cttcgagata
    17161 ttcagtgaga tcttccgcca tccgcttggt cagagttgta accagtacgc gttcgttaat
    17221 tgccgctcgc tgacgaatct ccgatagaag atcatccacc tgtgtcgcta ccggacgcac
    17281 ttcaataatc gggtcgagta agcctgttgg acgcacaacc tgatccacca catcgccacc
    17341 ggatttttcc agctcgtaat tacccggcgt cgccgaaaca tagatggttt gcggcgctaa
    17401 tgcttcgaac tcttcaaatt tcagcgggcg gttatccagc gctgatggca agcggaagcc
    17461 gtactccacc agtgtctctt tacgcgcccg gtcaccgcga tacatgccgc caatttgtgg
    17521 aatggtgacg tgagattcat cgaccaccag cagcccatca gcaggcaggt aatcaaacag
    17581 cgtcggcggt ggctcacccg gtccacgacc ggagaggaag cgcgagtagt tctcaatccc
    17641 cgaacagtag cctagctcgt tcatcatctc cagatcaaac tgggtacgct gggtcagccg
    17701 ctgctcttcc agcagtttgt tgttttccaa tagcacttta cgtctggcgg ccagctcttc
    17761 tttgatctcc tccatcgcct ggacgatgcg ctcgcgtggc gtgacgtagt gcgttttcgg
    17821 gtagatggta aaacgtggaa tagtggaaac aatctgcccg gtcagcgggt caaataacga
    17881 caatcgttcc acctcctcgt caaacagctc cacgcgaagt gcaatgtcat ccgattctgc
    17941 cgggaagata tcaatcacct cgccacgaac acggaaagta ccacgctgga atgcttgatc
    18001 attacgggca tattgcagct ccgccagtcg gcgcaggatc gcgcgctgat cgataatcat
    18061 accgaccgtg agatggagca tcatcttgag atataaatca ggatcgccca gaccataaat
    18121 cgcggaaaca gacgccacca caaccacatc acgtcgctcc agcatcgctt tggtggcgga
    18181 taaacgcatc tgttcgatat gttcgtttac cgaggcatct ttctcaataa aggtgtcgga
    18241 actcggtaca taggcctccg gctgatagta gtcgtagtag gagacgaaat attccaccgc
    18301 gttttctggg aagaattctt tcatttcgcc atacagctgg gccgccagcg ttttgttggg
    18361 cgcaagtacc atggttgggc gctgaaggtc agcaatgaca ttggcaatgg tgaaggtttt
    18421 cccggagcca gtcacgccaa gtaacgtctg gtgcgccagg ccatcttcca gcccctcttc
    18481 gagacgtcga atcgcctccg gctgatcgcc agaaggttta aaagcggaat tcagtttgaa
    18541 cggtttactc atgagtcgct acctgaagga gttgggcggg caggtatgta attttactcg
    18601 tcgtacttaa ttatgccaac aaattatact ggataaaaaa acagttcatc accataatat
    18661 ttctgttaca gcgtaaactc cgctgtcaat catgaacaag atttactctg cggcgagata
    18721 aaactccggc cttaccgggt tatccccaaa gcaacggctt ttttaacatt tgtcaagatg
    18781 attgatgaca gttttatggc aagagatgcc tgttcagtga ctgccattgc aattatctaa
    18841 cctgttaaaa aataaaagat atttctttga gctgtcttta acgccaattc gcgtgcaagc
    18901 cgcgtattct ctcgcttgcc tcgtgttttc taactctaat acacatggtt atccacagga
    18961 atagtggata actgtctgca gcccctatcc cccgccgcct gggcaattcc cacattcacc
    19021 gcatgatgcg gaggtaaaat ttttttcgtg atttttttag aatttaattg gttaaattgc
    19081 agtcaatcgg agacacgatc tcgctcgcaa tttaaccaat tacaggatga ctacattaag
    19141 gcaagattga tgtactttcc ggttgccgca ttttctggat tttctgcaag ccagggaatt
    19201 tcgcccagca gcggcgcggg aatcatgcgg gtgagcgtgg tcatatattc agcgtgacgt
    19261 tttcccggag gcgtgacatc gttcgccacc caccccgcca gagtcagtcc ggcgtgtagt
    19321 attgcctgcg cagtcaacat cgcgtgatta atacagccga gtttcacgcc cacaaccagt
    19381 atcactggca gttgttcctg tgttacccaa tccgcaaaag tgaaagtgtc agaaagcggc
    19441 gtaaaccagc cgccagcacc ttccactaac acccagtcag cctgttgttc aagcgcgcgt
    19501 aatccggcgc tcattaccaa tgcttctatc ggcctgccct cttgcgcgct gatgatatgc
    19561 ggcgaggtgg gttctgcgaa ggtgtaagga tttaccgttg cgtaatccag ctgcaggcta
    19621 ctgttgcgct gtaacgccag cgcgtcacta ttacgtaagc ctttcggggt cttttcgctg
    19681 ccagaggcca ccggtttgta acctgccgtc cggtagcctg ctgcctttgc ggcttgtaaa
    19741 agcgcgcagc tggcgacagt tttccccact tcggtatccg ttccggtgac aaaataacat
    19801 ttactcacga gcaatcactc ccaaaaaaag atgatacgtc agaggatatc gcccctgttg
    19861 ttgcggccag gccagttgca atcgctgcaa ctgcgaacgc gttaatattc gcgggtcacg
    19921 cccttcatga agatgcgtgg caccgatgcc tttcagcgaa cgcatggcac tgagcgcatc
    19981 atcaaaccac agcgtgatgg gctgaatata atgttgataa tgcacggcgt ttagcgactg
    20041 ttcgatttca tctggcggta aaaagcgatt agcatgcgga cgctcgtcca ccgcctgcca
    20101 cgcctgatgc agttcgggta acgatccctg caccagcgtg gtaaacgcga ccacgcctcc
    20161 ggggcgcacc acccgataca gctcgcggag tgccgtggat aaattaccgc accactgcac
    20221 cgcgagattg ctccatgcaa gatcgaacgt cgcagtcgct aacggcaggg attcgatatc
    20281 tcccgccaga taatggtctg cggcatcctt ctggcgtgcc tgaacaagca ttggcggaga
    20341 gagatctaac gcagtcacct gcgcgtgacg ctctcgccag cggcggctca tccagcctgg
    20401 tccacaaccc gcatccagta cgcgggtgta tttacgctgt gggagcattg ccagtaaggc
    20461 gtcagcactc tggcgctgta gatcggcgtg ttgctcatag tgtgcggctg cccgtccaaa
    20521 tgccgcagca atggcttgtt tattaaccgt tgccatgcag cacctccagc agacggtcga
    20581 tatcctgcat ttcatgcgct gcggttagcg ttaagcgcag tcgcgcagta ccagcgggta
    20641 cggttggcgg gcgaattgcc gtgacccaac agccttgctg gcgcagtttt tctgccagtt
    20701 gtaacgcacg gctgttatca ccgacgatca atggctggat ggcgctccat gaatcagcaa
    20761 gcgtaaacgg caaatcctgc actccggcac gaaaacgcgt tatgagtgcc gccagttttt
    20821 cgcgccgtgc atccccctca tcactgcgaa tgactgccag cgacgcacgt aatgcctgcg
    20881 cctgagcggg cggcatactg gtgctgtaga taagatggcg agcgaattgc agcagataat
    20941 ccgccaccgt attggagcaa agcaccgctg ccccgctgac gccaaatcct ttgccaaacg
    21001 tcaccaccag caattctggt tttacctttt gcagccagca gctgccacgc ccctgctccc
    21061 cgataacgcc cgtgccgtgg gcgtcatcga ccatcaacca gccatcgtgc tgttgcgtta
    21121 cctgctggat ttcctccagt ggcgcactat cgccgtccat gctgaacacg ccttctgtca
    21181 ctaccagttg ctgccccgga cagggggaag caagcagtcg cgccagatga gtgacatcgt
    21241 tatgagcaaa acggcgaagc ggcgacgggc ttaaacttgc agcctccagc aatgaggcat
    21301 ggctaagccg gtcggcaaca atacggtcct ctttcgccat catcgcggca atgactgcct
    21361 gattagcagc aaaaccagag ataaacagca gtgcccgcga atagcccagc cactcggcca
    21421 gttcttcttc cagcgcctga tgcgccacgc tataaccgct gacgtgaccg gagccgccgc
    21481 taccgatgcc aaattgctcc gcaccctgct tccaggcacg gataatttgc ggatgatggc
    21541 ttaaacctaa ataatcgtta ctggaaaagt tcagatactg gcgatcatcc accaccagcc
    21601 agcgtccggc tccttgcgcc accggataac ggcgacgcag ggcatcggca gcacgccgcg
    21661 catcgagcgc cgcgttgatt ttctcctgcc agctcatagt gctgccgcgt tgtaatattc
    21721 gtcagtgtcc ggggtcatca gcgcctgctc cagacgctgc tgttgttcgt tatcgcccgc
    21781 cagcacggca gtttgctgcg gatttagccc cagtttgcgg aacagttgca ggtctttatc
    21841 ttcttccgga ttcggcgtgg tcagcagttt gcaaccgtag aaaatcgagt ttgcgccagc
    21901 cataaagcac atcgcctgag tctgttcgtt catctgctcg cgtccggcag aaaggcgtac
    21961 gtaagaggtc ggcatcatga tccgcgcaac tgcaatggtg cgaataaaat caaaggcatc
    22021 gacatcatcg ttatccgcca gcggcgtgcc tttcaccttc accagcatgt tgattggtac
    22081 gctttccggc ggcgttggca ggtttgccag ttgcagcaat aatccggcgc gatcttttac
    22141 tgtttcgcct aagcccacaa tgccgcctga acagactttg atcccggcgt cgcgcacttt
    22201 ttccagcgta tcgaggcgtt cctgataggt acgcgtggtg atgatattgc cgtaaaactc
    22261 cggcgaggtg tcgaggttgt ggttgtagta atccagcccg gcattcgcca ggcgctgcgc
    22321 ctgagattca ctcaacgtgc ccagcgtcat acacgcctcc agccccatcg cttttacccc
    22381 ctgcaccatt tgttccaggt acggcatatc gcgttcgtgg ggattcttcc acgccgcgcc
    22441 catacagaaa cgcgtcgatc ccgccgcttt cgctttgcgc gccgactcca gcacctgttc
    22501 aacttccatc aaccgctcgg cttccagccc ggttttgtag cgcgagcttt gcgggcaata
    22561 tttgcaatct tccggacaag ctccggtctt aatcgacagc aaagtgctga cctgcacctg
    22621 acgaggatcg aaatgctgac gatgcacctg ctgcgcttca aacagcagat ccagcaacgg
    22681 tttttcaaat aattctgtga cttgcgacaa tgtccagcgt gggcggtgag ccatggggct
    22741 tctccaaaac gtgttttttg ttgttaattc ggtgtagact cgtaaaccta aatcttttca
    22801 atttggttta caagtcgatt atgacaacgg acgatcttgc ctttgaccaa cgccatatct
    22861 ggcacccata cacatccatg acctccccgc tgccggttta tccggtggca agcgccgaag
    22921 gttgcgagct aattttgtct gacggcagac gcctggttga cggtatgtcg tcctggtggg
    22981 cggcgatcca cggttacaat cacccgcagc ttaatgcggc gatgaagtcg caaattgatg
    23041 ctatgtcgca tgtgatgttt ggcggtatca cccatgcgcc cgccattgag ctgtgccgca
    23101 aattggtggc aatgacgccg caaccactgg agtgcgtttt tctcgcggac tccggctccg
    23161 tagcggtaga agtggcgatg aaaatggcgt tgcagtactg gcaggccaaa ggcgaagcgc
    23221 gccagcgttt tctaaccttc cgcaatggtt atcatggcga tacctttggc gcgatgtcgg
    23281 tgtgcgatcc ggacaactca atgcacaacc tgtggaaagg ctatctgcca gaaaacctgt
    23341 ttgctcccgc cccgcaaagc cgcatggatg gcgaatggga tgagcgcgat atggtgggct
    23401 ttgcccgcct gatggcggcg catcgtcatg aaatcgcggc ggtgatcatt gaaccgattg
    23461 tccagggcgc aggcgggatg cgcatgtacc atccggaatg gttaaaacga atccgcaaaa
    23521 tgtgcgatcg cgaaggtatc ttgctgattg ccgacgagat cgccaccgga tttggtcgta
    23581 ccggcaaact gtttgcctgt gaacatgcag aaatcgcgcc ggatattttg tgtctcggta
    23641 aagccttaac cggcggcaca atgacccttt ccgccacgct caccacgcgc gaggttgcgg
    23701 aaaccatcag taacggcgaa gccggctgct ttatgcatgg gccaactttt atgggcaatc
    23761 cgctggcctg cgcagcagca aacgccagcc tggcgattct cgaatctggc gactggcagc
    23821 atcaggtggc ggctattgaa gcgcagctac gcgagcaact tgcccccgcc tgtgacgccg
    23881 aaatggttgc cgatgtgcgc gtactggggg caatcggcgt ggtcgaaacc tctcgtccgg
    23941 tgaatatggc agcgctgcaa aaattctttg tcgaacaggg tgtctggatc cggccttttg
    24001 gcaaactgat ttacctgatg ccgccctata ttattctccc gcaacagttg cagcgtctga
    24061 ccgcagcggt taatcgtgcg gtgcaggatg aaacattttt ttgccaataa cgggaagtca
    24121 gcgtgagggt ttctggctac actttctgca aacaagaaag gagggctcat gaaactcatc
    24181 agtaatgatt tacgcgatgg cgataagctg ccgcatcgtc atgtctttaa cggcatgggt
    24241 tacgatggcg ataatatttc accgcatctg gcgtgggatg atgttcctgt gggaacgaaa
    24301 agttttgttg tcacctgcta cgacccggat gcgccaactg gctccggctg gtggcactgg
    24361 gtagttgtta atttacccgc ggatacccgc gtattaccgc aagggtttgg atctggtctg
    24421 gtagcaatgc cagacggcgt tttgcagacg cgtaccgact ttggtaaaac cggatacgat
    24481 ggcgcagcgc cgccgaaagg cgaaacccat cgctacattt ttaccgttca cgcgctggat
    24541 gtagaacgta ttgatgtcga tgaaggtgcc agcggcgcga tggtcgggtt taacgttcat
    24601 tttcactctc tggcgagtgc ctcgattact gcgatgttta gttaatcact ctgccagatg
    24661 gcgcaatgcc atctggtatc acttaaaggt attaaaaaca actttttgtc tttttacctt
    24721 cccgtttcgc tcaagttagt ataaaaaagc aggcttcaac ggattcattt ttctatttca
    24781 tagcccggag caacctgtga acacattttc agtttcccgt ctggcgctgg cattggcttt
    24841 tggcgtgacg ctgaccgcct gtagctcaac accgcccgat caacgtcctt ctgatcaaac
    24901 cgcgcctggt acctcttctc gcccgattct gtcggcaaaa gaagcgcaga atttcgatgc
    24961 tcaacactat tttgcatccc tgacaccagg tgcggcagcg tggaatcctt ccccgattac
    25021 cctgcctgcg caacctgact ttgttgtcgg cccggcgggt actcaaggtg taacgcatac
    25081 cacgattcag gcggcggtag atgcggcaat tatcaagcgt accaacaagc gccagtatat
    25141 tgccgtgatg cctggtgagt atcagggaac ggtgtatgtc cctgccgctc cgggtggaat
    25201 tactctgtac ggtacaggtg aaaaaccgat tgatgtgaag attgggcttt cccttgatgg
    25261 tggcatgagc cctgccgact ggcgtcacga cgtcaacccg cgcggcaaat atatgccagg
    25321 taaaccggcg tggtatatgt acgatagctg ccagagtaaa cgcagcgaca gtatcggtgt
    25381 tctctgctct gcggtcttct ggtcacaaaa caatggcctg caactgcaaa acctgaccat
    25441 cgaaaacacg ctgggcgata gcgtagatgc gggtaaccat ccggcggtgg cactgcgtac
    25501 tgatggcgac aaagtgcaga tcaataacgt caacattctc ggtcgtcaga acaccttctt
    25561 tgtcaccaac agcggtgtgc agaaccgtct ggaaacgaat cgccagccgc gtacgctggt
    25621 gaccaacagc tatattgaag gggatgtgga tatcgtttct ggtcgcggcg cagtggtgtt
    25681 cgataacacc gaattccgcg tggtgaactc ccgtacccag caagaagcgt atgtgtttgc
    25741 accggctacg ctgtccaaca tttactacgg tttcctcgcc gtaaacagcc gtttcaatgc
    25801 ttccggtgat ggcgtggcgc aactgggccg ctcgctggat gttgatgcca ataccaacgg
    25861 tcaggtagtg atccgtgata gcgccatcaa cgaaggtttt aacacagcca aaccgtgggc
    25921 tgatgcggtg atctctaatc gcccgtttgc gggtaacacc ggcagcgttg atgataacga
    25981 cgaagtacaa cgcaatctga atgacactaa ctacaaccgc atgtgggaat acaataaccg
    26041 cggcgtgggt agcaaagtgg ttgcagaggc gaagaagtag gagctgttaa ctatgtgccg
    26101 gatgcggcgt aaacgcctta tccggcctac agttcgatac gatttgtcgg tcggataaga
    26161 tgcgccagca tcgcatccga caataagtgc cggatgcgac ctacattcac atggcgcttt
    26221 ttacatctga cggtttttat tgaagttaat caaactaccc gccttgataa tctcgcgttc
    26281 ttcggcagtc agactttcca tatagagcgt gatttccgtt accggtgcat cttcatggat
    26341 cacataacct ttaaacgtcg ttccaggatt atccagcgcc gctttaatgc cagggatgta
    26401 aatgtaatcc cccacttcaa aggttggcgc ttccgccatc tgcagcggta acatccccca
    26461 gttgatgacg ttagaacgat agcgtttagt cgcgtactcc tcggcaatat tcgccagacc
    26521 gccaatcaca cgctgacaac tcgccgcctg ttcacgcgca gaaccatcgc ctggtttcac
    26581 cgcatagacc atgctgccaa tttcagtttg cagcggatca acatgttcct gaccagcaat
    26641 ctgcttaatg cgcgcaaaca cctctgccat ctcgctgaca ttccccgcca gacgctgatt
    26701 ttccagctca gcagtcgctt tacttctgcc aacataacct ggatcgcggc gtgacagggt
    26761 aaactccgcc agaccaatcg gatttgaacg ataagaagag gtttcaccgg aaggaatcag
    26821 ttcgtcggtg gtggtcactt cgtcgaggat cttcgagcac actttgagga cgatattgtc
    26881 agtcagcgca cccaattccg gccagtcttt aatgttcggt ccgtaaatca gcggttgctg
    26941 ggtcgcccct ttcacaaagc cttgatagac gcgattttta tacggcgtta catcgaaggc
    27001 gtactccggc acgttgtccc agcaatcgag ttcgctggca gaggttaaat agccaccgtt
    27061 tgccgcagtc gcagcgatag aacgagcgtc catcaacgcc accgctgaca tctgcccatt
    27121 agctggctta gagccttcgc ggttcgggaa gttacgcgtg gtgtggcgaa tactcaaacc
    27181 gttgttgatt ggcgtatcgc ccgcgccaaa gcatgggccg cagaacgcgg ttctgatgat
    27241 tgcgcctgcg ccaatcaaat ctgcgaccac accttttttg gcgagatcca taaacaccgg
    27301 ctgtgatgac gggtaaaccg ctaacgagaa ggtgtcattg ccgcaggatt gaccgcgcag
    27361 cgcattcgcc gccgcgatga cgttttcata gttaccgcca gaacagcccg cgataatccc
    27421 ctgctgcact ttcaggcgac cattttccac tttatccagc agcgagagtt tggctttacc
    27481 gtgcgccacg cgttcggact caatttcaat ctcacgcaga atgtcggtca ggttctggtt
    27541 cagcgtgtcg atttcataca cgttgctcgg gtggaatggc agcgcaatca ttggtttgat
    27601 ggcgcttaaa tcaacgctga tgcagccatc gtagtacgcc attggttgag ggttaagttg
    27661 gcagtaatcc tggccgcgac cgtgcagcgc cagccagtta tggacttctt catcggtttg
    27721 ccagacagaa cttaaacagg tcgtttcagt ggtcatcacg tcaacgctgt tacggaaatc
    27781 ggtagagagc gctgcaacac cgggacctac gaactccatc actttgtttt tgacgtaacc
    27841 gtttttgaac acggcaccga tgatagccag cgccacatcc tgcggcccca catacggcgc
    27901 aggttttccg gtcagatgca ccgcaaccac gcccggatag tcgatatccc aggtgtcatt
    27961 aagcagttgt tttaccagct cgccgccgcc ctcaccgact gccattgtcc ctaatgcgcc
    28021 gtaacgggtg tggctgtctg atccgaggat cattttgccg ccgccagcca tcatttcacg
    28081 catatattga tggatgaccg caatatgcgg tggcacaaaa ataccgccat aacgctgggc
    28141 cgccgataaa ccaaaaacat ggtcatcacc gttaatggtg ccgccgacgg cgcagagtga
    28201 gttatggcag ttggtcagca catacggcag cgggaaacgt tccataccgg acgctttagc
    28261 ggtctgtaca ataccaacaa aggtaatatc gtgagaggct aatgaatcaa acttaatttt
    28321 aagtttatcc atatttccgg acgtattatg agaagagaga atagaccagg caatagtgcc
    28381 ttttttggct tcttcttttt taatttcgcc ggtgaaatgt tcttcggcaa ttatttcgtt
    28441 attactggcg agaaacacgc ctttttcaga taacttgatc atcaaatgct ccagatatct
    28501 gcgcaccgga aagtgcgcag aaagataata aatggatata ctctaaataa ttcgagttgc
    28561 aggaaggcga caagcgagtg aatccccagt cacttacata agtaagtgac tggggtgagc
    28621 gaacgcagac gcagcacatg caacttgaag tatgacgagt atatttacag catgttccag
    28681 ccgatcagca tattccacca ccacacgccg agggtgatat gcaccaggaa ggttaatatc
    28741 gtcagtaccg caccgaccaa ccaccaggat ttaatatcgt tgtaacccac gccaaagatg
    28801 accggacctg ccgcgccgcc atagtgagta accatgccgc cataggagtt agagaacaac
    28861 agtgccagcg cggttaacat taacggtgcg ccggagacat tcgccagcat ggcaaatacc
    28921 ggtaacatgg caacgatata ggcactacca gaagcgaaga aataacgcac gatgatgctg
    28981 aggaaaataa taacgaagaa agcaacgttc ccgtgaccat caaatgccag gttattttta
    29041 aagacttcag ctaaccattc gaagaattta actttcgata ataaggagct taagccgata
    29101 ataccgccgt accagattaa ggtattccag ccgcctttat ttttaaccac gtcttcccag
    29161 gtaacgatac ccagcagcag catagttgcc ataacaacga ttgccacggt ggattcatca
    29221 acccccagag acttactgaa aatccagccc agcagcgcca gcacaaagac accgagcagc
    29281 attttttcgc ggattttcat cggtcccagt tcggcaaggc ccgctttagc gatggttttg
    29341 ttatccacct tcttaatttc tggtggatac atggtgtaaa tcaccagcgg ggtgaccagc
    29401 agcataatga tgcccggcaa tccggcggct aacgcccatc caccccagct aatttgcagg
    29461 tgcagaatgt cgttgatcat tttcagcgcc agaatgttcc ccgccattgc ggtaaagaac
    29521 atatagctgg tggttttggt gaccatgtaa atggacatca tcaggtaatg tccaacacga
    29581 cgcggacttt tttccggttc tgatcccaaa gccactgcca cgctgttgat gatcggtaac
    29641 acgatgccgc ccgcacgcgc ggtgttagac ggtgttgccg gagccagtac cagatcgaga
    29701 aataccgtaa cgtaacccag acccagggta gtgctaccaa ttttaccaat cagcagatag
    29761 gcaatacgtt tacctaagcc tgtggttaca aacgcggcgc ttaaagtaaa cgcagaaaac
    29821 accagccagg tggtaccgga agagtaaccg cttaatacag cggtggtttt aaattcccca
    29881 cctgataagt tacccaccac taccatcgat gcggcgacgg caattaacag tacgacaggt
    29941 tccgggaaag gcttgataac cagccccaca atggccgcca ggtaaatacc aaaaagcacc
    30001 cacgccagtt cgcttaatcc tgccggagct ggcataaagc caataataca tgggattgct
    30061 aatatcagaa ttagcttcca taacgatttc ttattcattt aatatcctgt tctttctgta
    30121 tgactcatac agaattcctt tattgattgt cattatcacg acaacaaaat ttttcaggga
    30181 agataaactt caccggaaaa tatttttctg gtcgtccgaa taacagatgc gcgtaacgtc
    30241 gtggcatcct gaccttcatt acttaaatga acgtcgagcg caccactggg gtgttcaata
    30301 ttgatattgc cgtatcctac agaagggacg atttgtcggg tgacggtacc ttccaatgca
    30361 caactactgg aaatagcaat agcaccggtt atcgccaaag cgcgatggca agaatgcggc
    30421 ataaaataac gcacattaat tgccccgcct ttctgcgctg gagaaataag tacaggttta
    30481 gggataacca tattactgac atcgcctaaa cccattgctt tacccgcttg tagacggata
    30541 gattcaatgc gggctaataa tgctttgtcg gcatccagct ctgccggtaa ttcatagcct
    30601 gtttttccca gatattcagc aggaataatg acaactggca tcgccatatc gatacaggtc
    30661 accgggacat cgtcaaaata atcaatctga ttatccgtcg ggaagacttt tccggttttc
    30721 gttccggcgg cattcaggaa agtgagcgca accggtgcgg cagtacccgg tacgccgtca
    30781 attctggcgc taccctcgta ctcgacaaca ccatttggcg tttgcacatc agcttcgatg
    30841 aacgtacccg tattgacgtt gcggatacgt acgcgggtaa ctggcgaagt cgctgcaatc
    30901 aaaccatttt caatggcgaa tgccccaacg ccagacagca tattgccgca gttaggcgtg
    30961 gtatcgacac gttgctcatg gacgataact tgagcaaaca gataatcgac atctgcacgc
    31021 ggatcgctgg agcggctaat aatggcgact ttactggtca gcggattacc gccgccaata
    31081 ccgtcaattt ccagatcgtt accggaaccc ataattgcca tcaatatttt atcgcgctgc
    31141 gtttgatctt cgggtagatg ttccgctaac aggaacgcgc ccctcgaggt tccacctcgc
    31201 atcatcacgc agggtatttt tttcataata atgccctcaa tttaaaaaca taacttattg
    31261 cgatatggtt acattaaggg caaagcatct ctaatgactt tagctcaacg attaataaca
    31321 ttttttcatt aatttaagtt ggctaaatgc agttaaaatt tttaactgcc agtcacccaa
    31381 aatcgtcgct tccattttcc ggagtaataa tgaagcatga attatcaagt atgaaggcat
    31441 ttgtcatact ggcagagtcc agttcattta acaatgctgc taaattactc aatattacgc
    31501 aacctgcatt aacgcgaaga ataaaaaaga tggaggaaga tttacacatt caactatttg
    31561 agcgtacaac tcgcaaggtt acgttaacaa aagcaggaaa aaggttgctc ccggaggcgc
    31621 gggaattaat aaaaaaattc gatgaaacgc tttttagtat tcgcgacatg aatgcttatc
    31681 atcgaggtat ggtgacgtta gcgtgtattc cgaccgcggt attttatttt ttaccattgg
    31741 caatcggtaa atttaatgag ctgtatccca atattaaagt gcggattctg gaacaaggca
    31801 cgaataattg catggaatcg gtgctgtgta acgaatcaga ctttggcata aacatgaaca
    31861 acgtcacaaa ttcatccatt gattttaccc cactggtcaa cgagccgttc gtgctggcct
    31921 gtcggcgtga ccatccattg gccaaaaagc agcttgtaga atggcaggag ttagtgggtt
    31981 acaaaatgat tggcgtacgc tcctccagcg gcaaccgact gttgatagaa caacagctag
    32041 ccgacaagcc ctggaagctg gactggtttt acgaagtgcg tcatctttcg acgtcattag
    32101 ggctggtgga ggcgggactg gggatttcgg cgcttcctgg tctggcaatg ccccatgcgc
    32161 cctattcttc gattattggt atcccactgg tggaacccgt tatacgtcgg acattgggga
    32221 ttattcgccg caaagatgcc gtgctttctc cggcagctga acgctttttt gccttgctga
    32281 ttaatttgtg gactgacgat aaagacaatt tgtggaccaa tatcgtcgaa cgccagagac
    32341 atgcgttaca ggagataggt taatacaccg gcgcaggaga gactgcgccg gttaaatcag
    32401 cggttagtgc gcgttgacca ctacccacat tggcccctgc ccaaccgcat agcggccttt
    32461 ttcatgcagt agcccctgct cgccaacaat ttcgtatacc gagatgtggt gagatttttg
    32521 cccggcggca atcagatact tgccgctgtg atcaacattg aagccgcgcg gctgggtttc
    32581 cgttggctgg aagccttctt tactcaacac gctgccatct tccgaaacgc tgaaaacggt
    32641 aatcagactg gcggtacggt cgcaggcgta taaatgacga ccatccgggg tgatatgaat
    32701 atcagccgcc caacgggtgt cggagaagtt ttccggcatc atatccagcg tctggacaca
    32761 ttcgatattg ccgtgcggat ctttcagttc ccagacatcc actgagctgt ttaactcatt
    32821 gacgcaatac gcatattgtt cgtttggatg gaataccata tgacgcgggc cggccccttc
    32881 aacggtagtc acttccgcag ggtcctgcgc caccagatga ccatcatcgc tgaccgtaaa
    32941 caggcaaatg cgatcctgct ttaatgccgg aacccacaac gtacggttgt ccggtgagat
    33001 attggcggaa tggcaaccgt ccagcccctc gaccacatcg acgacgccca ctggcaggcc
    33061 atcttccaga cgcgttacgc tcacgttacc cgcattgtaa gaaccgacaa agacaaactg
    33121 cccctggtga tcggtggaaa tatgcgtcgg gctacccggc agcgcagact ctgcagcaaa
    33181 ggtcagcgcg ccatcatccg gtgcaatacg atacgccaga acacgaaact ccggacggac
    33241 accaacgtag aggtaacgtt tgtccgggct gaccaccatc ggctgcacct gccccggcac
    33301 atcgacaacc tgtgtcagcg tcagtgcgcc ttcatgattc aggttccaga cgtgaatttg
    33361 ctggctctca gggctggcga tataaactgt ttgcttcatg aatgctcctt tgcatttagc
    33421 aactgactgt aacagctaaa attagtcgct tttggcggtg aatgctgaat taaaacgcag
    33481 aattttgtcc gatagtggta tcggtgtacc attcgcgcag acgaaaattc ttaaccttaa
    33541 tctggaatac gccatgacca cacgcgtgat tgctctcgac ttagacggca ccttattgac
    33601 cccgaaaaag accctgcttc catcatcgat agaagccctg gcccgcgctc gcgaagcagg
    33661 ctatcgatta atcatcgtca caggtcgcca tcacgtcgct attcatcctt tttatcaggc
    33721 gctggcgctg gatacacctg ctatttgctg taatggcacc tatttgtatg attatcatgc
    33781 aaaaaccgtg ctggaagcgg acccaatgcc cgttaataaa gccctgcaac tcattgagat
    33841 gctgaatgaa caccacattc acggtctgat gtatgtggat gatgcgatgg tctatgagca
    33901 cccgaccgga catgtcattc gcacgtctaa ctgggcgcaa accctgccgc cggaacagcg
    33961 tccgactttc acacaagtcg cttctctggc tgaaacggcg caacaagtta acgccgtatg
    34021 gaagttcgcc ctgacgcacg atgacctgcc gcaattgcag cattttggta agcatgtcga
    34081 acatgaactg ggactggagt gtgaatggtc ctggcacgat caggttgata ttgcacgcgg
    34141 cggcaacagc aaaggtaaac gtttgacgaa atgggttgag gcgcaaggct ggtcgatgga
    34201 aaacgtcgtg gcgttcggcg ataacttcaa tgatattagt atgctggaag ccgctggtac
    34261 aggcgtggcg atgggcaacg ccgatgacgc agtaaaagcg cgcgccaaca ttgtgattgg
    34321 tgataacacc accgacagca ttgcccagtt catttatagc cacctgattt aatcaggcgg
    34381 ttatcgacac acttttaatt tgcgcataca gccacaggcc aggtttgatc gccagttcat
    34441 ccctggccca cgggctgata cgcgcccaca gcgttttaca gccgacttcc agctccactt
    34501 ccacctggcc gttgtcgtca taactattaa caacttttgc ccgcaatacg ttacgaatgc
    34561 tggtttgctg cggcggttgt aaaaccagtg agacatcgga agcctgaatg cggatgcgta
    34621 gcgcagcttg cagcggttcg tccagcttat tgacccacaa atgctgatcg cccagcgcca
    34681 gcgcggtcat cgcgtaatgc ggatgatgct caagtactgt cactttcaga atgctacttt
    34741 gttgctcttt cggtagccac ggattcatca cgctactacc ccagacttct tccagcgcgc
    34801 caaaggcttt cacctgaccg ttttccagta ccatcactct gtctgccaga tggaggatct
    34861 catccagcga atggctgaca tacaacatcg gaatgttgat ttcccgcgtc agacgttgca
    34921 gataaggcaa cagttcgcgt ttacgcggaa tatccagtga tgccagcggt tcatccagca
    34981 acagcaattc cggtgctgtc agcaaagccc gaccaatcgc cacgcgctgt ttttcgcctc
    35041 cggacaggct gcctggtaaa cgatcaagca acggttcaat gcctaaaagc gccaccagct
    35101 tatcgaactg attgaccatg cttttcgcca tgccgtagcg caaattgcca cgcactttat
    35161 aatgcggaaa cagccgcgca tcctgaaaca catagcctac gcgacgcttt tctggcgtca
    35221 ggcagatacc tttatcggcg tcatttagta cccgaccatt gaggacaatc cgcccttttt
    35281 gcggacgtgt cagcccactg atggcgttaa tcagcgaggt ttttccggca ccagagacac
    35341 caaaaatagc ggtgatgcca ttggcgggca gcgtttcatt aatcgtcagg caatggttgc
    35401 ccaacgtctg ggaaaaattc agttccagca tgattagcgc cccgcccgct cacggctgat
    35461 tctggccagc cattctgaaa tcaacaggga gatcatcgcc agcgcaatag aaataataca
    35521 cagtctcgcc gcgccacttt ccccgccagg ggtctggatc agggtataca tggcagaagg
    35581 aatggtccgc gtttcaccag gaatgttcga cacaaaggtg atggttgcgc caaattcacc
    35641 gagcgaacga gcgaatgcca gtaccgtacc aacaataatt cccggtaagg tcagcggtaa
    35701 cgtgatagta aagaaaacgc gccagcgccc ggcccccagt gttcttgcgg cctgctccag
    35761 tttgatatca acaccttcca gcgccagacg aatcgcccgc accatcagcg gaaaagacat
    35821 gacagcggca gcgagaaccg caccacgcca gctaaacgcg aaagaaagac caaaccagtc
    35881 atacagacgt tcaccgataa atccgcgccg tcccatcgaa actaataata ggtaaccgac
    35941 gaccacgggc ggtaacacca gcggtagatg cagtacgctg tcgagcagag ctttgcccgg
    36001 aaacgtgcaa cgtaccagta accaggcaaa aaagatccca aacggcaggc taaacagcac
    36061 agccagggaa gaaactttca ggcttaataa aactgcctgc cattctggat cggtcagtat
    36121 cattacttgg ttgtaaatcc gtaacgttta aagatttccg cagcctgcgg ccccttcaga
    36181 taatcataaa atgctttcac tgtcgcattg ttatgccctt ccacaacagc aaccggatat
    36241 tccacttttt tatgtgaatc ttccgggaag gtggcaacca cttttacccc tttgctggca
    36301 actgcgtcag aaccgtaaac aatacccagc ggcgcttcat tgcgttcgac caacgccagc
    36361 gcgccacgaa catcttccgc cggagccagt ttcggggaaa gcgtatccca tgcgcccagt
    36421 ttttgcagtg cttctttcgc ataaatgcca gcagggacat gttccggatc accaaccgcg
    36481 aggcgaccgc cattcagcag cgaagtccag ttggttttgc tgtcgatggt gaaatctttc
    36541 tgctcgctgg ctttcggtgc tacaacgacc aggctattgc cgagcagtgt ctggcgcgaa
    36601 gccgtatcga tcgctttttt atcaaccgca taatccatcc atttctgatc ggcagaaata
    36661 aacagatccg caggcgcacc cgcttcaatc tgacgggcca aagtagatga cgaagcgaaa
    36721 gaagaaacca catccacgcc tttctctttt ttatactgcg tagcaatgtc ctgcattgcg
    36781 ttagtcagtg atgcggcggc gaacaccgtg attttccctt catctgccag tgcattgcca
    36841 gcaacagcga aagagagtgc cgccccggta aacaagttca accatttacg agccattggt
    36901 aaccccttaa tctaacgtta tgtaggcgtc aatataacgg caagttcgga attttcccag
    36961 cagttattgc taacctttgt gaggtagatg agaaaaaata tcggcagaaa aagcaggaag
    37021 ttgagaaaaa gaaaatgccc gactaagcgg gcattcaggg aatcaatgat tttgtccggg
    37081 ctggtctttt ttaccaacac cagaaaagat gttgaatact tcaccaagac cgtaaatcag
    37141 acccaggatg atggccatca cgacaggtac catgattacg gcgaatacca gactttttaa
    37201 taactctaac atggtcaact ccagatatag tcatgagact attctaaccg ctaagcacag
    37261 aaaagcactc cccttttgtg cggtcagctt tgcgtggcgt tcgttttccg tcacaataag
    37321 acttttgcca ggacattgtt atgcaggccg aaatccttct cacccttaag ctccaacaaa
    37381 aattattcgc cgacccgcgc cgcatttcgc tactaaaaca cattgcgctt tccggttcca
    37441 ttagccaggg agcgaaagat gccggtatca gctataaaag cgcctgggat gccattaacg
    37501 agatgaatca gttaagtgag catattctgg ttgagcgcgc aacaggcggt aaaggtggag
    37561 gcggcgcagt actgacccgc tatggtcagc gactgattca actctatgac ttactggcgc
    37621 aaatccagca aaaagccttt gatgtgttaa gtgacgatga cgccctgccg ctgaacagcc
    37681 tgctggccgc gatctcacgt ttttcactgc aaaccagcgc ccgtaaccag tggttcggta
    37741 ccatcaccgc tcgcgatcat gatgacgttc aacagcatgt tgatgtctta ctggctgatg
    37801 gaaaaacacg cctgaaagtc gcaattaccg cacaaagcgg cgcgcgtctg gggctggatg
    37861 aaggcaaaga agtgttgata ttgctaaaag cgccgtgggt aggtattact caggacgagg
    37921 cggtcgcgca aaacgctgac aaccaattac cgggtattat tagtcatatt gagcgcggcg
    37981 cagagcagtg cgaagtatta atggcgctac ccgacgggca aacactgtgc gccacagtgc
    38041 cggtaaatga agcgacttct cttcagcaag ggcagaatgt cacggcctac tttaatgccg
    38101 acagcgtgat tatcgccacg ctgtgctaag cgtgttgaca atttgttatg aaacacgtat
    38161 ccctgtcagt aatcgctgca caaaatggga tataaaatgt catcgttgca aattttgcaa
    38221 ggcacgtttc gtcttggcga cacaaaaacg ctgcaattgc ctcggctaac gttaaacgcg
    38281 ggtgatagtt gggcgtttgt cggttcgaat ggaagcggga aatcggcgct ggcccgcgcg
    38341 ctgtcggggg aacttccgct tttgaaaggt gaacggcaaa gccagttttc ccacatcact
    38401 cgtctctcct tcgagcaatt gcaaaagctc gtcagcgacg aatggcagcg gaataacacc
    38461 gatatgctta gtcctggcga agatgacacc gggcgtacca cggctgagat cattcaggat
    38521 gaagtaaagg atacttcgcg ttgcatgcaa ctggcgcagc agttcggtat taccgccctc
    38581 ctcgaccgac gctttaaata cctttccact ggcgagacgc gaaaaaccct gctgtgtcag
    38641 gcgctgatgt cggagcctga cttgttgatc cttgatgaac cgtttgatgg cctggatgtt
    38701 gcctcacgtc agcatctggc tgagctactc gcctcgttac atcagtccgg tattactctg
    38761 gtactggtgc tcaatcgctt cgatgagatc ccggaatttg tccagtttgc tggcgtgctg
    38821 gcggattgca cgttagcgga aactggcgct aaagaagaac tgctccaaca agcactcgtt
    38881 gcgcaactgg cgcatagcga actgcttgaa ggtgtgcaaa tgccggagcc ggatgaacct
    38941 tcagcacgtc acgccttacc cgccaacgaa ccgcgcattg tgctgaacaa tggcgtggtt
    39001 tcttataacg aacgccccat ccttaataac cttagctggc aggtgaatcc aggcgaacac
    39061 tggcaaattg tcgggccaaa tggcgcggga aaatcgacgt tattaagcct gattactggc
    39121 gatcatccgc aaggttacag caacgatttg acgcttttcg gacgacgtcg cggcagcggc
    39181 gaaaccatct gggatatcaa aaagcatatc ggttacgtca gcagtagttt gcatctggat
    39241 taccgggtca gcactactgt gcgtaatgtg attctttctg gctattttga ttcgattggc
    39301 atttatcagg ccgtttcgga tcgccagcaa aaactggtgc agcagtggct ggatattctc
    39361 ggcattgata aacgcacggc tgacgctcct ttccatagtc tttcctgggg acagcagcgt
    39421 ctggcgctga ttgtccgcgc actggtgaaa catccgacgt tgcttattct cgatgaacca
    39481 ctacaggggc ttgatccgct caatcgccag cttatccgcc gttttgttga tgtgctgatt
    39541 agcgaaggtg aaacgcaatt gttgtttgtt tcgcaccacg ctgaagatgc gcctgcctgt
    39601 attacccatc gtcttgagtt cgtgccggac ggtggactct atcgctatgt gctgacaaaa
    39661 ataaattgag tcggtaatgc tgaccgtgcc ggaggcggcg taaacgtgtt atccggccaa
    39721 cggtttgacg catccaggca tgaaaccgcg tctttttttt cagataaaaa gcgcaatcat
    39781 tcataaagcc gctgttttat aatcacttaa tcgcgcataa aaaacggcta aattcctgtg
    39841 taaacgattc cactaattta ttccatgtca cacttttcgc atctttgtta tgctatggtt
    39901 atttcatacc ataagcctaa tggagcgaat tatgagagtt ctggttaccg gtggtagcgg
    39961 ttacattgga agtcatacct gtgtgcaatt actgcaaaac ggtcatgatg tcatcattct
    40021 tgataacctc tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa
    40081 acatccaacg tttgttgaag gcgatatccg taacgaagcg ttgatgaccg agatcctgca
    40141 cgatcacgct atcgacaccg tgatccactt cgccgggctg aaagccgttg gcgaatcggt
    40201 acaaaaaccg cttgaatatt acgacaacaa tgtcaacggt actctgcgcc tgattagcgc
    40261 catgcgcgcc gctaacgtca aaaactttat ctttagctcc tccgccaccg tttatggcga
    40321 tcagcccaaa attccatatg ttgaaagctt cccgaccggc acaccgcaaa gtccttacgg
    40381 caaaagcaag ctgatggtgg aacagatcct taccgatctg caaaaagccc aaccggactg
    40441 gagcattgcc ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg gtgatatggg
    40501 cgaagatccg caaggcattc cgaataacct gatgccgtac attgcccagg ttgctgtagg
    40561 ccgtcgcgac tcactggcga tttttggtaa cgattatccg accgaagacg gcactggcgt
    40621 gcgcgattac atccacgtaa tggatctggc agatggtcac gtcgtggcga tggaaaaact
    40681 ggcgaacaag ccaggcgtac acatctacaa cctcggcgca ggcgtaggca gcagcgtgct
    40741 ggacgtagtt aatgccttca gtaaagcctg cggcaaaccg gttaactatc attttgcacc
    40801 acgtcgcgcg ggcgaccttc cggcctactg ggcggacgcc agcaaagccg accgtgaact
    40861 gaactggcgc gtaacgcgca cactcgatga aatggcgcag gacacctggc actggcagtc
    40921 acgccatcca cagggatatc ccgattaagg aacgaccatg acgcaattta atcccgttga
    40981 tcatccacat cgccgctaca acccgctcac cgggcaatgg attctggttt caccgcaccg
    41041 cgctaagcgc ccctggcagg gggcgcagga aacgccagcc aaacaggtgt tacctgcgca
    41101 cgatccagat tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga
    41161 ttacaccggg acttacgttt tcactaatga ctttgcggcc ttgatgtctg acacgccaga
    41221 tgcgccagaa agcaacgatc ctttgatgcg ttgccagagc gcgcgcggta ccagccgggt
    41281 gatctgcttt tcaccggatc acagtaaaac gctgccagaa ctgagcgttg cggcattgac
    41341 ggaaatcgtc aaaacctggc aggagcaaac cgcagaactg gggaaaacat acccgtgggt
    41401 gcaggtcttt gaaaacaaag gcgcggcgat gggctgctct aacccgcatc cgcacggaca
    41461 gatttgggca aatagcttcc tgcctaacga agctgagcgc gaagaccgtc tgcaaaaaga
    41521 atatttcgcc gggcagaaat caccaatgct ggtggattat gttcagcgcg agctggcaga
    41581 cggtagccgt accgttgtcg aaaccgaaca ctggttagcc gtcgtgcctt actgggctgc
    41641 ctggccgttc gaaacgctac tgctgcccaa agcccacgtt ttacggatca ccgatttgac
    41701 cgacgcccag cgcagcgatt tggctctggc gttgaaaaag ctgaccagtc gttatgacaa
    41761 cctcttccag tgctccttcc cctactctat gggctggcac ggcgcgccat ttaatggcga
    41821 agagaatcaa cactggcagc tgcacgcgca cttttatccg cctctgttgc gctctgccac
    41881 cgtacgtaaa tttatggttg gttatgaaat gctggcagaa acccagcgag acctgaccgc
    41941 agaacaggcc gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg aatccggagt
    42001 gtaagaaatg agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc
    42061 tgccactcac accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta
    42121 caacgacggt ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gctgtgcgcc
    42181 acgcgatgac cgtaaagttc gcgtaatggc agccgattat gaaaatcagc tcgacgagtt
    42241 ttccctcgat gcgcccattg tcgcgcatga aaactatcaa tgggcgaact acgttcgtgg
    42301 cgtggtgaaa catctgcaac tgcgtaacaa cagcttcggc ggtgtggaca tggtgatcag
    42361 cggcaatgtg ccgcagggtg cagggttaag ttcttccgct tcactggaag tcgcggtcgg
    42421 aaccgtattg cagcagcttt atcatctgcc gctggacggc gcacaaatcg cgcttaacgg
    42481 tcaggaagca gaaaaccagt ttgtaggctg taactgtgga atcatggatc aactgatttc
    42541 cgcgctcggc aagaaagatc atgccttgct gatcgattgc cgctcactgg ggaccaaagc
    42601 tgtttccatg ccgaaaggcg tggctgtcgt catcatcaac agtaacttca aacgtaccct
    42661 ggttggcagc gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca
    42721 gcagccagct ctgcgcgatg tcaccattga agagttcaac gctgttgcac atgaactgga
    42781 cccgatcgtc gcgaaacgcg tgcgtcacat cctgactgaa aacgcccgca ccgttgaagc
    42841 tgccagcgcg ctggagcaag gcgacctgaa acgtatgggc gagttgatgg cggagtctca
    42901 tgcctctatg cgcgatgatt tcgaaatcac cgtaccgcaa attgacactc tggtagaaat
    42961 cgtcaaagct gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggtgg
    43021 ctgtatcgtc gcgttgatcc cggaagaact ggtgcctgcc gtacagcaag ctgtcgctga
    43081 acaatatgaa gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg
    43141 agcaggacag tgctgaacga aactcccgca ctggcacccg atggtcagcc gtaccgactg
    43201 ttaactttgc gtaacaacgc agggatggta gtcacgctga tggactgggg tgcgacttta
    43261 ctttccgccc gtattccgct ttccgatggc agcgtccgcg aggcgctgct tggctgcgcc
    43321 agcccggaat gctatcagga tcaggccgca tttctggggg cctctattgg tcgttatgcc
    43381 aaccgcatcg ccaatagccg ttataccttt gacggtgaaa ccgtgacgct ttcaccaagt
    43441 cagggcgtca accaattgca tggcggcccg gaagggttcg acaaacgtcg ctggcagatt
    43501 gtgaaccaga acgatcgtca ggtgctgttt gccctgagtt cagatgatgg cgatcagggc
    43561 ttcccgggta atctcggcgc gacggtgcaa tatcgtctga ccgacgataa ccgtatctcc
    43621 attacttatc gcgccacagt tgataaacct tgcccggtga atatgactaa tcacgtctat
    43681 ttcaatcttg acggcgagca gtctgacgtg cgcaatcata agttgcagat tctggcggaa
    43741 gaatatctgc cggttgatga aggcggcatt ccgcacgacg gcctgaaatc tgtcgccgga
    43801 acctcttttg atttccgcaa cgccaaaatc atcgccagtg agtttcttgc cgacgacgat
    43861 cagcgcaaag tgaaaggtta cgatcacgcg ttcttgttac aggccaaagg cgatggcaag
    43921 aaagtggcgg cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt ctacaccacc
    43981 gctccggctc tgcaattcta ctccggcaac ttcctcggcg gcacaccgtc gcggggaacc
    44041 gaaccttacg ctgactggca agggctggct ctggaaagcg aatttctgcc ggacagcccg
    44101 aaccaccctg aatggccgca accggactgc ttcctgcgtc ctggcgaaga gtattccagc
    44161 ctgacggaat atcagtttat tgctcagtaa tgttaagccc tcgtcatgag ggctttatcc
    44221 tcatattgtt caaatcacca gcaaacaccg acatatttgc aactcaatat tcacaacaac
    44281 cttacactgc gacactattt tcgctatggt tatgcgtaag cattgctgtt gcttcgtcac
    44341 ggcaatataa tgagaattat tatcattaaa agatgatttg aggagtaagt atatggctgt
    44401 aactaagctg gttctggttc gtcatggcga aagtcagtgg aacaaagaaa accgtttcac
    44461 cggttggtac gacgtggatc tgtctgagaa aggcgtaagc gaagcaaaag cagcaggtaa
    44521 gctgctgaaa gaggaaggtt acagctttga ctttgcttac acctctgtgc tgaaacgcgc
    44581 tattcatacc ctgtggaatg tgctggacga actggatcag gcatggctgc ccgttgagaa
    44641 atcctggaaa ctgaacgaac gtcactacgg tgcattgcag ggtctgaaca aagcggaaac
    44701 cgctgaaaag tatggcgacg agcaggtgaa acagtggcgt cgtggttttg cggtgactcc
    44761 gccggaactg actaaagatg atgagcgtta tccgggtcac gatccgcgtt acgcgaaact
    44821 gagcgagaaa gagctgccac tgacggaaag cctggcgctg accattgacc gcgtgatccc
    44881 ttactggaat gaaaccattc tgccgcgtat gaagagcggt gagcgcgtca tcatcgccgc
    44941 acacggtaac tcgttacgtg cgctggtgaa atatctcgat aacatgagcg aagaagagat
    45001 tcttgagctg aatatcccga ccggcgtgcc gctggtttat gagtttgacg agaacttcaa
    45061 accgctgaaa cgctattatc tgggtaatgc tgacgagatc gcggcgaaag cagcggcggt
    45121 tgctaaccag ggtaaagcga agtaaacgtc attcgcttaa aaatgagaaa gccgactgca
    45181 aaatgagtcg gcttttttat ttttcgttgc taccaatgga gcgcattgcc tgatgcgacg
    45241 ctgcgcgttt tatcaggcct acggtgatta atcggatacg ccactctgac agcgcatccg
    45301 acaattaaac tttacccgcg acgcgctttt actgcattcg ccagttgacg taacagagca
    45361 tcggtatcat cccagccaat gcaggcatcg gtgatgctct taccgtaggc cagcggttcc
    45421 ccgctctcga ggctctgatt gccttccacc agatggcttt ccaccatcac gccaataatg
    45481 gccttttcgc caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg
    45541 aattgtttag acgagttagc atggctgaaa tcgatcatca cctgtgctgg caggcctgct
    45601 ttgttcagcc cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg
    45661 ccgcgcagaa tgatatggca atcgccgtta ccgctggtat tcacaatcgc cgaatgaccc
    45721 catttggtca ctgaaaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc
    45781 actttaatcg taccatcagt gccatttttg aagcctaccg ggcaagaaag ccctgaagcc
    45841 agttcgcggt gcacctgcga ttccgtagta cgtgcgccaa ttgcgcccca gctcatcagg
    45901 tcagcgagat attgtggggt gatcatatcg agaaactcac ccgccgctgg cagaccgctg
    45961 tcgttaatat caagcaacaa tttacgggca atacgcagac cgtcgttgat ctggaagctg
    46021 ttatccatat gcggatcgtt aatcagccct ttccagccca ccgtggtacg cggcttttca
    46081 aaatagacgc gcattacgat ttccagctca tctttcagct cttcacgcag cgccagcaag
    46141 cgagtggcat actctttagc cgcgacagga tcatgaattg agcatgggcc aatcaccacc
    46201 aacaggcgat catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcgacc
    46261 gtattcgcgg cattttcagt agcggggaat ttttccagca atgcgacagg aggaagtaac
    46321 tctttgattt ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg
    46381 ccatacttat cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa
    46441 cggggtttta cactatgaac ggattaatcc tggaattagc aagaaaaacg ccagattgtc
    46501 gcgaaaaacg agatctctcc tacaatttct aactgtaact cctttgtttg ttaattattt
    46561 caagattctc tgctgggtat ctttgcctgg ctgaaatctt aaaccattgc cttatattca
    46621 cctgcaaatg cactgttgga agaggttatc cgacataacg accataacag gagcatccta
    46681 tgaaaatgac aaaactggcc acactatttt tgactgccac tctaagcctt gccagcggtg
    46741 ccgcactggc tgccgatagc ggagcgcaaa ctaataacgg ccaggcaaac gccgccgctg
    46801 atgcgggcca ggtagcccct gatgcccgtg aaaatgtcgc gccaaataac gtcgacaata
    46861 acggggtaaa taccggttct ggcggcacaa tgctgcatcc ggatggttct tcaatgaaca
    46921 atgacggcat gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgctgcc
    46981 cggatatcaa taaaaaagtc caaaccggtg atggcatcaa caatgatgtc gataccaaaa
    47041 ccgacggtac cacgcagtaa ccctgcgaaa tagccaataa tgggagagcg tagtctctcc
    47101 cttttaattt ttcccttcta aaagcagtat gatgtcataa agttaagtga gaataattaa
    47161 gggatgactc gtcatggcgc actcacactc acacacttcc tcacacctgc cagaagataa
    47221 taatgctcgt cgcttgttgt atgctttcgg cgtgactgcc gggtttatgc tcgtagaagt
    47281 cattggtggt tttctttccg gttctctggc attgctggcc gatgcgggtc atatgttgac
    47341 cgatactgcc gccctgcttt tcgccctact cgcagtgcaa ttttcccgtc gtcctcccac
    47401 cattcgccac actttcggtt ggctaagatt gaccacgctc gcggcgtttg tgaatgccat
    47461 cgccctggtg gtgattacta ttttgattgt ctgggaggcg atagaacgct tccgcactcc
    47521 gcgtccggtc gagggcggca tgatgatggc aattgccgtg gctgggctgc tggcaaatat
    47581 actttctttc tggttacttc atcacggcag cgaagagaaa aacctcaacg tgcgagcagc
    47641 ggcactgcat gtgctcgggg acctgctggg ttcggttggg gctattattg ccgcgttgat
    47701 tattatctgg accggctgga cacctgctga ccccattctc tcaatactgg tgtcgcttct
    47761 ggttctgcgt agcgcgtggc gactgttgaa agatagtgtg aatgaattac ttgaaggtgc
    47821 accggtatcg ctggatatcg ctgaactgaa gcgtcgcttg tgccgggaaa tcccggaagt
    47881 ccgcaatgtg caccatgtgc atgtatggat ggtaggcgag aagccggtga tgacgctgca
    47941 tgtgcaggtg atcccgccac acgatcacga tgccttgttg gatcagatcc aacactacct
    48001 gatggatcac tatcagattg agcacgccac cattcagatg gaatatcaac cttgtcatgg
    48061 gccagactgc cacctgaata gaggggtgtc tggccattca catcatcatc attaatggga
    48121 cagcgcacgt gaacctcttt cgcgtgcgct tttgatccac atccggctgc cgttgagggc
    48181 aataaaggtc aggatgatgt actccagaga catggcgtaa acgccctgaa gtgcaaaaat
    48241 aacgacgcta atcacgttaa taatcaccca caacagccag ttttccacat acttacgtgt
    48301 catcagaatc attgccacga tagataacac catcatgcat gaatcccaga acgggaaagc
    48361 gtccggctgt agttcaggca tcgcaacctg taatcctaat gcttgcatga tcatgactgc
    48421 cacgcgggtc aaaaatgcaa acacaggatt gataaatacc gtcattagac caatcgaaac
    48481 aacgcaaacc gccagccagc ttagcgcctt cggcaatggc aaccagcgaa ttttcaactc
    48541 cgcctcgttc tgactggttt gtcgcgacca cgcataccag ccgtaaatat tcgcggcaaa
    48601 gaaaaacacc tgtaataaca ggctggcata cagctgaatc tgaaagaaaa taatgccaaa
    48661 caaggtgacg ttgatcaggc caaagaagta gttactgatc ttctccagac tggcaaggcc
    48721 aatacacagc aacccggcga tcgtgcctac cgcttcgatc catgagagat cataaccgcc
    48781 tgcccctatt ggtatatgta ccaggatatt ctgcacacta aaaaaatcca tcgttccccc
    48841 aaagcgtaat gtgacgcctt attatcaatt atccacgtag tgtagccgca aaatccagca
    48901 tacgattgag cggcaccagc gccctctctc gcagcctttc atcaacataa acctcgtgat
    48961 tgcttccttc cagttctaat gcctctgcga tggcctgaag gccattcatg gccatccacg
    49021 gacaatgcgc gcagctgcga caggttgcgc cctcacccgc ggttggtgct tccagtaact
    49081 ctttatctgg caccgcctgc tgcattttgt agaaaatgcc ccgatcggtt gccacgataa
    49141 gccgctgatg ttgcaatgtt ttcgcagcag cgatcagttg actggtggaa ccgaccgcat
    49201 ccgccatctc tacaatagct tgcggtgatt ctggatgcac cagtatggca gcatccgggt
    49261 attcttcttg caagcgggtt aacgcctgag tcttaaactc atcatgcaca atgcaggcac
    49321 cctgccagca taaaatgtct gcgcccgtct gtttttgcac gtaacgcccc agatgtttgt
    49381 caggtgccca gatgattttt tcacccaaac tatccagatg atcaataagt tcgacggcaa
    49441 tgcttgaagt taccacccaa tctgcgcgcg cttttaccgc agcagaagtg ttggcgtaga
    49501 cgacgacagt acgatcggga tgggcatcgc aaaatgcgtt aaattcttca acagggcagc
    49561 cgagatccag tgaacattca gcctgaagtg tcggcatcag aattgttttt tccggactga
    49621 gaattttggc ggtttctccc ataaatctca ccccagcgac tagcaacgta gaagcgggat
    49681 gctttgcacc gaagcgcgcc atttccagag aatcagaaat acagccaccg gtttcttctg
    49741 ccagttgttg aatttcggga tcggtatagt agtgggcaac catcaccgca ttacgttctt
    49801 ttagtagacg ttttatcttc tcgcggtaat tcgctttttc atcaatgctt aacggcgtcg
    49861 gcttcggggg gaaaggataa atcgccgtgt ctggatcaaa catcacgctc atcttaccat
    49921 ctcgttctac aggcttaacg ttaaaaccga cttaagcgtg atattcgcta ttatcggcgt
    49981 aatttgtttt atatactaaa caagatagtc gaatggtggg gaaaagtcac tcgaattacg
    50041 tatgagatgc cgaatgccac actttgcgtc ttattcctcc gacgtagagt tgtttacgca
    50101 gcaaacgcgg agtatgtcat cagggtcaag aattgaaatt gagagatgcc tgaatagcaa
    50161 aaaaacgcct ttagggcgct tttttgcatt ggtgggtcgt gcaggattcg aacctgcgac
    50221 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccatacg ggtgttacct
    50281 gaaagatttc tactcggaac caatgtttaa aattggtggg tcgtgcagga ttcgaacctg
    50341 cgaccaattg attaaaagtc aactgctcta ccaactgagc taacgacccg agtggtgggt
    50401 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
    50461 aatcacccga tactgcgctg gatactacaa actacttcca ccgaacccac cgtaatcagt
    50521 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
    50581 ctgagctaac gacccggtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
    50641 agggaggtgc tctcccagct gagctaatca cccgatacta cgctggatac tacaactaac
    50701 tatgttagtg gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg
    50761 ctctaccaac tgagctaacg acccactttt tcgttgcttt cggtttgttt gatatcccgt
    50821 ggcaacggcg gcatatatta ctgatttcag acttgagcgc aacaaaaatt tcgatgtaga
    50881 tcactcaact gcttatgatt cgcacgacac gaccagaaat aatgcgactt ctggtcgtgt
    50941 gttatgcatt acatcgcgtt cagacgtttt tgcgcctgtt tagcgccatc agtaccaggg
    51001 tatttactga taacctgctg gtatacggct ttcgcttttg cggtgtcacc tttgtcctgc
    51061 atgatgacgc cgactttaaa catcgcatct gcagccttcg gtgacttcgg atagtttttc
    51121 actaccgaag caaaataata cgccgcatca tcttttttac ccttgttgta gtttaactga
    51181 cccagccaat aattggcgtt tggcaggtaa gttgaatcag ggtaattttt gatgaaattc
    51241 tgaaatgcca ccattgcgtc atcctggcgg gatttatcct gcaccagcgc aatagctgca
    51301 ttgtaatccg tgtttgcatc accgcttttc accggcgcgc cagcattcga agtaccagca
    51361 tcagctgtcg gcgtcgttga tgccaccaca ccgctttgat cgccgctggt tgattgcgcc
    51421 gctgcaccac cgctgctgag gctgtcgatc tgcaacagga tctgcttttg ccgctccacg
    51481 acctgattca gttgatactg attttcctga atctgaccac gcagggaatc aatatcggat
    51541 tgattatcag agagttgttg ctggagttgg gttaaaagct ggctgtgagc attagaaata
    51601 cgctcaagtt gagtgacgcg gtcttcgacc gagcctgagc cgacactact gattggtgcc
    51661 tgagcaaaag cggcccaggg ggccgctata ccaaccagta acgacagact caatagttga
    51721 tgtctgaagt tactgctcat gcaattctct tagtaaacca gtaccgcacg acggtttttg
    51781 gcatacgccg cttcgtcatg acccagtact gcaggttttt ctttaccgta agaaacgatg
    51841 gagatctggt cagcagaaac gcctttaccc tgcagataca tcttaacagc gttcgcacga
    51901 cgttcaccca gggagatgtt gtattccgga gtaccacgtt cgtccgcgtg accttctacg
    51961 gtgactttgt aagacgggtt gctacgcagg aagtttgcgt gtgcatccag catttgagcg
    52021 aagtcagaac ggatatcgta cttgtccaga tcgaagtaaa cgatgttgtt ctgctgcagc
    52081 tgttgcattt gcagacgagc ctgctcttcg gaagacatgt tgccgttgcc gccgttcgca
    52141 tccataccag tgccggcacc cagcatgcct tcgctgccgt cattgctggc gttcttgttg
    52201 gaagaacatg ccgcaattgc cataacaggc agagcaatca tcagcccttt cagcactttg
    52261 ttcagttgca tttcaatgat tcctttacta ttcaattaat tattatcaca gatacggcga
    52321 ccaggcaggg aatttgacct gtccatcagt tgccggaaga cgcgctttga aacgcccatc
    52381 tgtagaaacc aaattcagca cggatcccat cccctgagaa gagctgtaga ttaccatagt
    52441 gccgttaggt gccagacttg gcgtttcatc caggaacgtg gacgacagaa cttgtacgcc
    52501 tcccgttgcc agatcttgtt tggcaatgtg ctgctgccca ccgttggagc tgaccattac
    52561 cataaattta ccgtcgctgc tgacatccgc atcctggttc tgcgaacctt cccaggtaat
    52621 acgttgtggc gcaccgccgt tgatattcac tttataaacc tgcggacgac cggcctggtc
    52681 agaagtaaat gccaggttct ggctgtccgg gaaccaggtc ggttcggtat tgttactgcg
    52741 accatcagtt acctggcgga tctgaccaga agccaaatcc attacgtaca ggttcaggct
    52801 accggttttc gacaaggcga atgccagttt gctgccgtct ggcgagaatg caggtgcacc
    52861 gttgtgacgc gggaatgaag ccacctgacg tacagcgcca tttgccagcg tctgaataac
    52921 cagcgcggaa cgaccgcttt cgaaggtcac ataagccagt ttagaaccgt ctggtgacca
    52981 cgccggagac atcagcggtt gcggtgaacg gtgaacgaca aactggttgt aaccgtcata
    53041 gtcagatacg cgcagttcat acgggaactg accgccgtta gtctgaacaa cgtaggcaat
    53101 acgggtacgg aacgcacctt taataccggt cagtttttca aatacttcgt cactggcggt
    53161 atgaccagca taacgcagcc actgcttgtt cactttgtac gagttctgag caagtacagt
    53221 acccggtgcg ccgccagtgt caacaagttg ataagcaaca ttgtaagaac catccggatt
    53281 cggagtgacc tgaccgacaa ccacagcgtc aatgcccagc gcggaccatg cagctggttg
    53341 tacttcctgc gcactacccg gctgctgtgg cagacgagca cgatctaacg gattaaattt
    53401 accgctgtta cgcaagtcag cagcaacgat gccgccaata tcttcaggtg ccgcaccagg
    53461 ccccgcccac tggaaaggaa caacaccaat aggacgaccg gaatctacac cgctgtcgat
    53521 cacaatgcgg acttcagcat gcagaactga tgcccacagt atgagaaaac caaatgctac
    53581 tcgtaatgcc tgcttcatca tatctccctt atctggaccg atggcccacg ataatttagc
    53641 agaatgttaa caaactaaaa tacacaaaac taccagaacc ccgtggcaac cggtgcctga
    53701 tgttgaccgt cggaacagtc aacatcgcga ttacggtttg aagtccaatg gcgcgttttt
    53761 gaacacttca tatactgcct ggcttggtgg tttcgggatc ttcgcaagtt tagctgctgc
    53821 caacgcagct tgacaaagtg cgggatcgcc accttcaggt ttgatatcca gtaacatacc
    53881 atcgggtgcc agttttatgc gcagcgtaca ggttttgcct gcataggacg atgcgtcata
    53941 gaacttactt tcgatagcag atttaatctg cccggcatag ttattgatat cggcccctga
    54001 tgcgccattg tttttagtat taccactccc ggcaggcgaa gcattgttcc ctttcgcccc
    54061 tccccccgtt ttcggtgcat tcttaccaga gcttagctca ccgaaaatat catctgcctc
    54121 agcggcagct tttgctgcag cggctttttc agctgctgct tttgccgctg ccgctttttt
    54181 gtctgcggcg gctttttctg ccgctgcttt cttatcagct gctgcctttt cagcagccgc
    54241 tttttctgct gcttttttgt cggctg
//
LOCUS       DAOFSV010000014        53002 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.014, whole genome shotgun sequence.
ACCESSION   DAOFSV010000014 DAOFSV010000000
VERSION     DAOFSV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.014"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(11..2173)
                     /gene="uvrD"
                     /locus_tag="QBH27_000944"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(11..2173)
                     /gene="uvrD"
                     /locus_tag="QBH27_000944"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDQ7013291.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(2257..2973)
                     /gene="yigB"
                     /locus_tag="QBH27_000945"
     CDS             complement(2257..2973)
                     /gene="yigB"
                     /locus_tag="QBH27_000945"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDQ7013292.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(2973..3869)
                     /gene="xerC"
                     /locus_tag="QBH27_000946"
     CDS             complement(2973..3869)
                     /gene="xerC"
                     /locus_tag="QBH27_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDQ7013293.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(3866..4573)
                     /locus_tag="QBH27_000947"
     CDS             complement(3866..4573)
                     /locus_tag="QBH27_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDQ7013294.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            complement(4570..5394)
                     /gene="dapF"
                     /locus_tag="QBH27_000948"
     CDS             complement(4570..5394)
                     /gene="dapF"
                     /locus_tag="QBH27_000948"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDQ7013295.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGIGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5431..5634)
                     /locus_tag="QBH27_000949"
     CDS             complement(5431..5634)
                     /locus_tag="QBH27_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ7013296.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            5793..5978
                     /locus_tag="QBH27_000950"
                     /pseudo
     CDS             5793..5978
                     /locus_tag="QBH27_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            6104..6424
                     /gene="cyaY"
                     /locus_tag="QBH27_000951"
     CDS             6104..6424
                     /gene="cyaY"
                     /locus_tag="QBH27_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDQ7013297.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(6464..9010)
                     /gene="cyaA"
                     /locus_tag="QBH27_000952"
     CDS             complement(6464..9010)
                     /gene="cyaA"
                     /locus_tag="QBH27_000952"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDQ7013298.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            9397..10338
                     /gene="hemC"
                     /locus_tag="QBH27_000953"
     CDS             9397..10338
                     /gene="hemC"
                     /locus_tag="QBH27_000953"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDQ7013299.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            10335..11075
                     /gene="hemD"
                     /locus_tag="QBH27_000954"
     CDS             10335..11075
                     /gene="hemD"
                     /locus_tag="QBH27_000954"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDQ7013300.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKCALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            11097..12296
                     /gene="hemX"
                     /locus_tag="QBH27_000955"
     CDS             11097..12296
                     /gene="hemX"
                     /locus_tag="QBH27_000955"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDQ7013301.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            12299..13495
                     /gene="hemY"
                     /locus_tag="QBH27_000956"
     CDS             12299..13495
                     /gene="hemY"
                     /locus_tag="QBH27_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDQ7013302.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            13545..14135
                     /locus_tag="QBH27_000957"
     CDS             13545..14135
                     /locus_tag="QBH27_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013303.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLTLFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            14765..16420
                     /gene="aslA"
                     /locus_tag="QBH27_000958"
     CDS             14765..16420
                     /gene="aslA"
                     /locus_tag="QBH27_000958"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDQ7013304.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(16511..17746)
                     /locus_tag="QBH27_000959"
     CDS             complement(16511..17746)
                     /locus_tag="QBH27_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDQ7013305.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCGFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLANETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPELNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17893..17969)
                     /locus_tag="QBH27_000960"
     tRNA            complement(17893..17969)
                     /locus_tag="QBH27_000960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17933..17935),aa:Pro,seq:tgg)
     gene            complement(18012..18098)
                     /locus_tag="QBH27_000961"
     tRNA            complement(18012..18098)
                     /locus_tag="QBH27_000961"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18062..18064),aa:Leu,seq:cag)
     gene            complement(18119..18194)
                     /locus_tag="QBH27_000962"
     tRNA            complement(18119..18194)
                     /locus_tag="QBH27_000962"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18159..18161),aa:His,seq:gtg)
     gene            complement(18253..18329)
                     /locus_tag="QBH27_000963"
     tRNA            complement(18253..18329)
                     /locus_tag="QBH27_000963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18293..18295),aa:Arg,seq:ccg)
     gene            complement(18432..19817)
                     /locus_tag="QBH27_000964"
     CDS             complement(18432..19817)
                     /locus_tag="QBH27_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ7013306.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(20008..20748)
                     /gene="rffM"
                     /locus_tag="QBH27_000965"
     CDS             complement(20008..20748)
                     /gene="rffM"
                     /locus_tag="QBH27_000965"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDQ7013307.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20751..22103)
                     /gene="wzyE"
                     /locus_tag="QBH27_000966"
     CDS             complement(20751..22103)
                     /gene="wzyE"
                     /locus_tag="QBH27_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDQ7013308.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGAAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(22100..23179)
                     /gene="rffT"
                     /locus_tag="QBH27_000967"
                     /gene_synonym="wecF"
     CDS             complement(22100..23179)
                     /gene="rffT"
                     /locus_tag="QBH27_000967"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDQ7013309.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHIAALRAVYQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQTLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLVSVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(23176..24426)
                     /gene="wzxE"
                     /locus_tag="QBH27_000968"
     CDS             complement(23176..24426)
                     /gene="wzxE"
                     /locus_tag="QBH27_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDQ7013310.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(24428..25558)
                     /gene="rffA"
                     /locus_tag="QBH27_000969"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(24428..25558)
                     /gene="rffA"
                     /locus_tag="QBH27_000969"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDQ7013311.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNINETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(25563..26237)
                     /gene="rffC"
                     /locus_tag="QBH27_000970"
                     /gene_synonym="wecD"
     CDS             complement(25563..26237)
                     /gene="rffC"
                     /locus_tag="QBH27_000970"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDQ7013312.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLIEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(26215..27096)
                     /gene="rfbA"
                     /locus_tag="QBH27_000971"
     CDS             complement(26215..27096)
                     /gene="rfbA"
                     /locus_tag="QBH27_000971"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDQ7013313.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(27115..28182)
                     /gene="rffG"
                     /locus_tag="QBH27_000972"
     CDS             complement(27115..28182)
                     /gene="rffG"
                     /locus_tag="QBH27_000972"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDQ7013314.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDTLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(28182..29444)
                     /gene="wecC"
                     /locus_tag="QBH27_000973"
     CDS             complement(28182..29444)
                     /gene="wecC"
                     /locus_tag="QBH27_000973"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDQ7013315.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRANELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(29441..30571)
                     /gene="wecB"
                     /locus_tag="QBH27_000974"
     CDS             complement(29441..30571)
                     /gene="wecB"
                     /locus_tag="QBH27_000974"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDQ7013316.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30627..31673)
                     /gene="wzzE"
                     /locus_tag="QBH27_000975"
     CDS             complement(30627..31673)
                     /gene="wzzE"
                     /locus_tag="QBH27_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDQ7013317.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31685..32788)
                     /gene="wecA"
                     /locus_tag="QBH27_000976"
     CDS             complement(31685..32788)
                     /gene="wecA"
                     /locus_tag="QBH27_000976"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDQ7013318.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(33028..34287)
                     /gene="rho"
                     /locus_tag="QBH27_000977"
     CDS             complement(33028..34287)
                     /gene="rho"
                     /locus_tag="QBH27_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDQ7013319.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(34372..34473)
                     /gene="rhoL"
                     /locus_tag="QBH27_000978"
     CDS             complement(34372..34473)
                     /gene="rhoL"
                     /locus_tag="QBH27_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDQ7013320.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34614..34943)
                     /gene="trxA"
                     /locus_tag="QBH27_000979"
     CDS             complement(34614..34943)
                     /gene="trxA"
                     /locus_tag="QBH27_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDQ7013321.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            35074..36339
                     /gene="rhlB"
                     /locus_tag="QBH27_000980"
     CDS             35074..36339
                     /gene="rhlB"
                     /locus_tag="QBH27_000980"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDQ7013322.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            36475..37959
                     /gene="gppA"
                     /locus_tag="QBH27_000981"
     CDS             36475..37959
                     /gene="gppA"
                     /locus_tag="QBH27_000981"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDQ7013323.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPPQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFEQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFANRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(38006..40027)
                     /gene="rep"
                     /locus_tag="QBH27_000982"
     CDS             complement(38006..40027)
                     /gene="rep"
                     /locus_tag="QBH27_000982"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDQ7013324.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            40114..40395
                     /gene="ppiC"
                     /locus_tag="QBH27_000983"
     CDS             40114..40395
                     /gene="ppiC"
                     /locus_tag="QBH27_000983"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDQ7013325.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(40441..41916)
                     /gene="ilvC"
                     /locus_tag="QBH27_000984"
     CDS             complement(40441..41916)
                     /gene="ilvC"
                     /locus_tag="QBH27_000984"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDQ7013326.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            42066..42956
                     /gene="ilvY"
                     /locus_tag="QBH27_000985"
     CDS             42066..42956
                     /gene="ilvY"
                     /locus_tag="QBH27_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDQ7013327.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(42953..44497)
                     /gene="ilvA"
                     /locus_tag="QBH27_000986"
     CDS             complement(42953..44497)
                     /gene="ilvA"
                     /locus_tag="QBH27_000986"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDQ7013328.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(44500..46350)
                     /gene="ilvD"
                     /locus_tag="QBH27_000987"
     CDS             complement(44500..46350)
                     /gene="ilvD"
                     /locus_tag="QBH27_000987"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDQ7013329.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(46415..47344)
                     /gene="ilvE"
                     /locus_tag="QBH27_000988"
     CDS             complement(46415..47344)
                     /gene="ilvE"
                     /locus_tag="QBH27_000988"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDQ7013330.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(47364..47627)
                     /gene="ilvM"
                     /locus_tag="QBH27_000989"
     CDS             complement(47364..47627)
                     /gene="ilvM"
                     /locus_tag="QBH27_000989"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDQ7013331.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(47624..49270)
                     /locus_tag="QBH27_000990"
     CDS             complement(47624..49270)
                     /locus_tag="QBH27_000990"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDQ7013332.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(49273..49323)
                     /locus_tag="QBH27_000991"
     CDS             complement(49273..49323)
                     /locus_tag="QBH27_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013333.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(49410..49508)
                     /gene="ilvL"
                     /locus_tag="QBH27_000992"
     CDS             complement(49410..49508)
                     /gene="ilvL"
                     /locus_tag="QBH27_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDQ7013334.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            49861..51381
                     /locus_tag="QBH27_000993"
     CDS             49861..51381
                     /locus_tag="QBH27_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDQ7013335.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(51406..51744)
                     /gene="maoP"
                     /locus_tag="QBH27_000994"
     CDS             complement(51406..51744)
                     /gene="maoP"
                     /locus_tag="QBH27_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDQ7013336.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            51863..52702
                     /gene="hdfR"
                     /locus_tag="QBH27_000995"
     CDS             51863..52702
                     /gene="hdfR"
                     /locus_tag="QBH27_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDQ7013337.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(52798..52873)
                     /locus_tag="QBH27_000996"
     tRNA            complement(52798..52873)
                     /locus_tag="QBH27_000996"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52838..52840),aa:Trp,seq:cca)
     gene            complement(52882..52958)
                     /locus_tag="QBH27_000997"
     tRNA            complement(52882..52958)
                     /locus_tag="QBH27_000997"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52922..52924),aa:Asp,seq:gtc)
BASE COUNT        12883 a        14473 c        13505 g        12141 t
ORIGIN      
        1 gaatcgtaaa ttacaccgtc tccagtcggg cgtaagccgc caccagccat ttaataccct
       61 ggccctggaa tgccacctgc aaacggctat gctcgccgct gccttccata ttgacgatgg
      121 tgccttcacc aaacttagcg tggcgtacgc gctggccgag cttgtagccg ctgtcgttct
      181 cgaccatcgg cgtccccata cgctgatggc tgaccgggcg gcttaccgtg gcgcgcaggc
      241 gcacctcttc cacacactct tccggcaact caccgataaa gcgcgacggg cgatggtaaa
      301 cctctttgcc atatagacgg cgggtttccg cgtaggtcag cgtcagtttc tgcatcgcgc
      361 gggttacgcc aacgtaggcc agacggcgtt cttcttccag acgcccgcct tcatctagcg
      421 acatctggct tgggaacatg ccctcttcca taccaacgat aaacacctgc gggaattcca
      481 gacctttcgc cgagtgtagc gtcatcaact gcaccgcgtc ctgccaggta tccgcctgcc
      541 cctcgcccgc ttccagcgcc gcatgggaga ggaatgcctg cagcggcatt aaatcttcgt
      601 cttcttcgtt gtagctgaac tggcgcgttg ccgtcaccag ttcctctaag ttttcgatac
      661 gcgtctgacc tttttcgccc ttctcctgct cgtacatggt gcgcaggccg gagtctttaa
      721 ttacccggtc agtctgtaca tgcagcggca tatcggcagt ttcctgcgct aaggcgtcga
      781 tcagctccat aaaccgctgt aaggcgctgg cagcacgtcc ggcgagggct ttttcctgca
      841 acagttcacg acatgcctgc cagagtgtta actggcgatc tcgcgatgtc tgacgtacca
      901 cgtccagcgt ccggtcacca ataccccgcg ttggcgtatt caccacacgc tcaaaggccg
      961 cgtcgtcgtt gcggttggca atcaggcgca gatacgagag cgcatctttg atttcctggc
     1021 gttcgaagaa gcgcatcccg ccgtaaatac gatacggcat actcgcctgc aataacgcct
     1081 cttccagcac acgcgactgg gcgttgctgc ggtagagaat ggcgcactca gcgagcgccc
     1141 cgccgttgtc ctgccaggtt ttgatgcgat taaccacaaa acgcgcttcg tcgagttcgt
     1201 taaaagcgca ataaagggaa atcggctcac cgtccgcgcc atcggtccac agttttttac
     1261 ccaaacgccc gttgttgttt tcaatcaggg cgttcgcggc gctcagaata ttgctggtag
     1321 agcggtagtt ctgctccaga cggatagttt cagcaccggg gaaatcattg aggaaacgct
     1381 gaatattctc cacctgcgct ccgcgccagc cgtagattga ctgatcgtca tcgccaacga
     1441 tcatcacttt gccggtatcg cccgccagca ggcggatcca cgcatactga atgttgttgg
     1501 tatcctggaa ttcgtccacc aggatattgg taaaccgttc gcgatagtgc tgaaggatat
     1561 gcggcttgtt aagccacaac tcgtgagcac gtagcagcag ctcggcgaag tccaccaggc
     1621 ccgcgcggtc acacgcttcc tgatacgcct gatacacctt ctgccaggtc tgctccaccg
     1681 gattaccgta gctctgaata tgatgcggac gcagcccctc atctttctgg ctgttgatgt
     1741 accacattgc ctgacgcggc ggccactgct tctcgtcgag attcatcgcc ttgatcaaac
     1801 gcttaagcag acgtagctgg tcttcactgt cgaggatctg gaaatcctgc ggcaggttgg
     1861 cgtccatatg gtgcgcacgc agcaggcggt gcgccagccc gtggaaggtg ccgacccaca
     1921 taccgccctg gctagtgccc atcagttgcc cgatacgatg acgcatctcc gccgccgctt
     1981 tgttggtaaa cgtcaccgcc ataatcgaat acggcgagca gttttccacg ctcatcaacc
     2041 aggcgatacg atgcaccagt acgcgcgtct taccactgcc cgcgcccgcc agcaccagaa
     2101 ggttgctgcg tggcgcggcc accgcttcgc gctgtttgtc attaaggctg tcgagcaggt
     2161 aagaaacgtc cattggcacc gccgcgtaaa aataggttgg gcggagaagc gcaacgccct
     2221 ccgccaaaaa gctgggtata tatacagatt tgctgattat atcagcgagg tcagagatgc
     2281 caaccgggaa atttccagat gcggcagtaa acggctgtcc caggtttgca tcagatcgcc
     2341 attttccggt ctgatccaac aggcctgcat tccgctgcga attgccccac ccacgtcagt
     2401 ggtgagatcg tccccgacat gtaagatctc gccgatcggc acgttgagtt tttccgccgc
     2461 caggaagtac atatcgctga acggttttga gcgcccgtgc gggccagcgc gcagcacaaa
     2521 ctcaaaataa tcccccaaac caaacagttc cggctgggcg ttaccgttgg tgatcgccac
     2581 cagcggccat ttcttcgcca gctgttttaa ggtgtcgtgg gtttgctgcg ggacgtcgat
     2641 ccggctgcgc catttggcaa agttgatcat tgctgcatgt gcgcctgcac tggcttcttc
     2701 ggcactcagc ccggcgtcga gcatcgcttg ttcaatcgaa cgaaaacgcc agcgcgtcac
     2761 gtcgtgataa atctcaggtt ccgcttcccg taccgcctgg cgcaggcgtt gcagatcttc
     2821 attctggaag ctgcgcagcg ccggatgata attttgcaca aaggtaagcg cctctcgctc
     2881 ggtgcgcaaa atcaccggac ggttatcgta aagggtatca tccaggtcaa aggtgagcgc
     2941 cgagatgcgc cccaaaggcc ggtaaaaacg cattatttcc cccgtttggc gcgtggatgc
     3001 gccgcatcgt acaccgaggc aaggtgttga aaatcaagat gagtatagat ttgcgtggtg
     3061 gagaggttgg catgacccag cagctcctgc acaccacgaa gatcgccgct cgactccagc
     3121 atatgggtag cgaacgagtg acgtaattta tgcgggtgaa cgtgattatt cagcccttgt
     3181 tttatgcccc attcggcaaa gcgtttctgc acattacgcg cggagatgcg tttgcccagt
     3241 ttcgacagaa aaagcgcgtc gtcttcgcta ccaaacaggt cgcgcaaatc aagccagtgc
     3301 tcaatccacg ccacggcgtt gcgaccaatc ggcaggcggc gctctttgct gccttttccc
     3361 atcacccaca cttcgccaga ctccaggtcg aggtgtttga tatcgagccc caccagctcg
     3421 gaaagacgca gacccgcgcc gtacatcact tccagcattg cccgatcgcg cacggcgagg
     3481 ggatcgttga tatcaatatc cagcagccga ttcatatcat cgacgtcgat gtttttcggc
     3541 agatgacgcg gcgctttcgg tgccgaaaca cctttcgccg ggttagcttt gagttcattc
     3601 tggctgacca gccagtcaaa aaagctacgt agcgcagaaa gccgtaacgc cagacttgca
     3661 gctcccagcc ctttacggcg actgcgtaca gcaaaattac gcaccatcgt cacatcacat
     3721 tgctgccagc tttgcaggcc gttttcgctg gcaaaatgga tgatcgcctc aagctgtcgc
     3781 tggtagttaa gcagggttat cgggctaagc tggcgctcca cgctcagata acgtaggtag
     3841 cgttctacat cggtgtgtaa atcggtcata cgcgttcaat ccaacgcgcc agaagctccg
     3901 gcaacatcag cgcaatttca tgaagtaact gcgttccctg cccttgttga tagtgactgg
     3961 catcgcgact ggtaaacagc acaaccccca aatcagcatc gcttcccagc atcgacatcg
     4021 ccaccgatcc caccgctttc gcttccggta gcaccaccag cagctccggt ccgttaagcg
     4081 gtcccagata gtgctgttcc tgccccagac gctgaatacg cagcggttcg aaagactgac
     4141 ggcttaatgc cagatgagtg tggttcgacg gcgcacctaa gcgccagcga tctggaaaca
     4201 ggcgcagact cgcacctgcc aggccgagat cgcgcgccca gcggtgaaag cgcatcagca
     4261 tatcatcgag actgctggcg gcggtgagac tgcgctgcag gtagagtaga cgataaaaca
     4321 ggccttcgtt ggcgatagcc tgttccatca acaacgccat gttctcttcc agaacatgaa
     4381 tatgattacg tgcgcgggcc atgtgccact cgaccaacga aacggtgccg cgtaccggat
     4441 gcggcacacg tatcgcttct actgcgcgcg cattgcggat aaaaaactca ggatttttaa
     4501 tcagataatc gacaaccgcc cggtcatcaa gctccgtgag tgtttcctgc agttcttccc
     4561 ctggttgctt catagatgaa taaatccgtc gtagacatgt accgccgggc cagtcatata
     4621 taacgggtga cccggacctt tccaggcgat atcaagacga ccgccaggga gttccacgcg
     4681 tacttcttcg gccagcaaac cttgctgaat ccctaccgcc accgccgcac acgcgccgct
     4741 gccgcaggcc tgggtttctc ctgccccacg ctcataaacg cgtaaacgaa tatgctcgcg
     4801 cttaaccact tgcataaaac cgatattggc gcgctccgga aaacgttcgt ggctttccag
     4861 aacaggacca agcgtttcta ccgccgcagt atcaacatca tcaacctgaa tcacgcagtg
     4921 cggatttccc atcgacacca cgccgcataa gattgtctgc tcggcggcgc gcataatata
     4981 ggtcttttcc gctttgttag cgcgaaatgg cacggcagaa ggttcgaagt tgggttcgcc
     5041 catattgacg cggaccagat catcatcggt gacggtcaac accatccgtc cgttggcggt
     5101 gctgacgcgg atatcgcgct tattggtcag ccctttcagg cgcacaaaac gggcaaagca
     5161 gcgcgcaccg ttgccgcact gcgctacttc actgccatca gcattgaaaa tgcgatagtg
     5221 aaaatccagt tccggatcat acggtggctc aaccaccagc agctggtcaa accctatccc
     5281 caggtgccga tccgccaggc gacgaatcag ctccggtgaa aaaaagacat tctgcgttac
     5341 cgcgtcgacg accataaaat cgttgccaag gccatgcatt ttcgagaact gcatcattta
     5401 ctccaatcac gcgggtacag aaactgactt ttagtaattc acctgggatg gaccatcgcc
     5461 agtggcgcgg tcgtttttgt ccggcaccgt ggattgcgtt tgcgtctcta ccggtttggt
     5521 cggcggcggt gcgtttttat ctgcaggcgg gaaatagagc ggacctttca gaccgcagcc
     5581 cgtcaggctg aagagagtaa gtaatacagt gagtgcctta aacacgtttt tcattatgga
     5641 ttgcctgtaa agttcatcgc tttctgcttt ctatcatcgc aggagaaggc gtaaaagcaa
     5701 gcgtttagcg actctttatg aagtctgaaa agtgaggaaa tgctgcgagt ataacgctta
     5761 ctcgtggcga tttatgtttt gctggtagtt tcgtgatcgg cacctcgcgc caggatggct
     5821 ttatgcacta tcgcaatgtg gtttgtttat tgtcttgttc gttgttctta tcatcggcat
     5881 ggggatgtcg gcttgatgag ccggaacata atatttacca gaaacaggga aaaggcgtgg
     5941 tgtatttgcg cccttatgaa aagaccaatc tttcgctata tagagatatt attattccgg
     6001 tgacggatga atcatgccgg aacgcagcag cagacgggca aactttgaag ccctgacccc
     6061 atgccgctat actgccgcca tcacttaaaa acaggataca acaatgaacg acagtgaatt
     6121 tcatcgcctg gctgatcaac tgtggctgac cattgaagaa cgtctggacg actgggatgg
     6181 cgacagcgat atcgactgtg aaatcaacgg cggtgtacta accattacct ttgagaacgg
     6241 cagcaaaatc attatcaacc gccaggaacc actgcaccag gtatggctgg caaccaaaca
     6301 gggcggctac cattttgatc tgaaaggcga tgagtggatt tgcgatcgca gcggcgaaac
     6361 cttctgggat ttgctggaac aggcggcgac gcagcaggcg ggagaaaccg tcagttttcg
     6421 ttaagagcta atgccggata agcctcgctt tccggcatta gcatcaagaa aaatactgct
     6481 gcaacagcgg cgcgtcatgc tcctgattgg caggtggcat gttaccgata gatttggtgc
     6541 gaaacggaat cacctgttca cgaccatcaa ccttcacaat ctgatagaac tgcggcaggt
     6601 tgaagttgat gaagcttgag ccgtaggtga aacggtcgtg cgaagacgag tagaagcgac
     6661 tgacgtcacg caccagttcc tctttgctgc cttcgcagtg gtgatacacc tcaacccggt
     6721 tgctttcgtc gagaatgtag atattaaagc cattctcgtc ttgcgtttct tcgaagaaga
     6781 actggatgat cccttcgctg gcaaagccgt ccaccaccgc cggtaatttg acgtgattgg
     6841 tttcaacctg cactgacagg ccgtgcagtt tgttatgcga aatagcgcca taaaactcga
     6901 tggcgttttc cagtttctgt accgatacat tcagccgttc gaagaacaag ccccaggttt
     6961 gaccggagac gcgcagcgcc ttgaagcgcc cggtttcctg gcgggtgctg gaaagacgca
     7021 gttcaataca ctcagaaacc agttgctgca cgcgagtacg gattaacccg cgcagatgct
     7081 ggctataaca gaagacttcc acgctatctg gcggtgcggc gtcctgatgc attttgccga
     7141 gaatagtttt cagggcttcg atcatcgatt gctcgccgtt gaagtgcagc gtacgcactt
     7201 cgttccacga gttgcgatac agcaggtcaa cgctacccac caggcaattt tgattctcgc
     7261 caaagctgaa gacatccagc ttacggaaat caaaatgcac cacctgattg cggaaggctg
     7321 ccgtcgggtc atattccagg ttaacgataa tcgccagatg gcggatctca cacgggctgt
     7381 agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa atggtgcgac acgtctgcga
     7441 ccatctcctg caacttaggc aaatcgacaa tgccgttacc tttaatatac aaacgggtgc
     7501 gagaggtcag caggccgtta aaccatgccc acgccaccag tttattcagg tagcgattat
     7561 attccagcgg ctgatggctg atgattgact caatatttgg cgcgcggtta tacagatacc
     7621 aacctgaacg gttggcgcgg cccggcggta cataaataaa ggtcagattc ggttccgaga
     7681 gatcgggcga aatctgcggg tttaccagcg tcactttacc cggtaatgct tcaaacgcgg
     7741 cgtacagttt acgtgtcagc acgccgatat cctgcgggct ggcggagacg ctaaggttat
     7801 tgcggcgcgc aaagcggatc agattacggt agctctgcat cattgcgtcg agcagctcgt
     7861 tgtgcgcctc acgcacctga tctatcttcc agttagcgcg gttatcgagc attgccagac
     7921 gagcttcgtc ccaaccccac tcgctcacta actggctcaa cactgcgcga cgccagccta
     7981 cacaggcgcg ttcacggctg agcttttcgc acacttttaa atagaagcag cgacgcacta
     8041 aatccagacg ggtgaaatct tcaatcgccg tcaggtattc agtaacacgc tccagcatca
     8101 tgcagtacgg gtcgaggcca aacgatacaa tctcgccgtc gtgcaaacgc tgtttgatat
     8161 ctttcgccag cagacgtggg ttcgggtatt cccaggaata ggcttccagc agcagtgttt
     8221 tcagcaccgc tttgtatggg gaatcgatac tcttgtagag ctgccaaagg ctggcaccaa
     8281 agtactcttc agcagaaagc gagcttaagc caccaagatc cagccattca tttggcgtca
     8341 gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta atgctcttct tcgtcgcacg
     8401 gcaccatatt ccacagaata cgcttaccgg cgagacgcac ggcggtacga taaaattcgt
     8461 caagcagcag tatatgctgg gtggagccac aatcttcgcc ccccaggctg ccgctttcat
     8521 tatgacggaa gcggttttca tcaatcagga agaagctgac ttccacaccc agcgaggcgg
     8581 cccagctttc cagcagacta catttacgtt gtagcaactg gcgctcttcg ctatcgagcc
     8641 aggattgatg acagacccag atatccaggt cagaggaaca actttgccct acggacgagg
     8701 tgctgcccat ggtgtataca ccagtaattg gaagctcacc tttcggtggg tcctgtactg
     8761 acattccacg atacagttca agctcgttca ggtagtggcg ttgagtttca tcaggcgtgt
     8821 aaaggcaaat gcctttggga acgttaccat caaggtaacc cggcattagc ggatggtgat
     8881 agtgcaacaa tgtcggcagt agactgtaga cctgttggaa tgcaggcccc atagcagcaa
     8941 gcgcgcgatc cacacgcaat tgatttatgg catccagtct ctgtttcaga gtctcaatat
     9001 agaggtacaa gacgtatcgc ctgatttgct acccgtcatg actgtgattc cgccaacatc
     9061 aacggtaaca cgcggcattc gggatatttc gtatgtcaaa ggtaaccgtt accacttttc
     9121 gcgcctggtt tttttagttt cacgacgaaa aaatggtcta aaacgtgatc aatttaacac
     9181 cttgctgatt gaccgtaaag aaagatgcgc tacatacaag tgtagcaccg tttattctct
     9241 gtaaattcct tattacgaag gctcaaagcg cctgtcagga tccactgccc gacctcattt
     9301 tacggtttgc gcaggcgtct acgtttcacc acaacactga catcgctctg gcaaggatgt
     9361 taggatggac cacggatgat aatgacggta acaagcatgt tagacaatgt tttaagaatt
     9421 gccacacgcc aaagcccact tgcactctgg caggcacact atgtcaaaga caagttgatg
     9481 gcgagccatc cgggcctggt cgttgaactg gtaccgatgg tgacgcgcgg cgatgtgatt
     9541 cttgatacgc cgctggcgaa agtaggcgga aaaggcttat ttgttaaaga gctggaagtc
     9601 gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcga tgaaagatgt gccggttgaa
     9661 ttcccgcaag gtctggggct ggtcactatt tgcgagcgtg aagatcctcg cgatgcattt
     9721 gtgtccaata actatgacag tctggatgcg ttaccggcag gcagtatcgt cgggacgtcc
     9781 agtttacgtc gccagtgcca actggctgaa cgccgcccgg atctgattat ccgctccctg
     9841 cgcggcaacg tcggcactcg cctgagcaaa ctggataacg gtgaatacga tgccatcatt
     9901 cttgccgtag ccggactaaa acgtttaggt ctggagtccc gcattcgcgc cgcgttgcca
     9961 cccgagattt ctcttccggc ggtagggcaa ggtgcggtgg gcattgaatg ccgccttgat
    10021 gatgcacgca ctcgcgagct gctcgccgcg ctgaatcacc acgaaactgc actgcgtgtt
    10081 accgccgaac gcgccatgaa tacccgtctc gaaggcggat gtcaggtgcc aattggtagc
    10141 tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgt tggtcggcgc gccggacggt
    10201 tcgctgatta ttcgcggtga acgccgcggt gcgccgcaag atgccgaaca aatggggatt
    10261 tcgctggcag aagagctact gaataacggc gcgcgcgaga tcctcgctga agtctataac
    10321 ggagacgccc cggcatgagt atcctggtca cccgcccgtc tcccgcagga gaagagttag
    10381 tgagccgtct gcgcacactg gggcaggtgg cctggcattt tccgctgatt gagttttctc
    10441 cgggtcgaca attaccgcaa cttgctgatc aactggcagc gctgggagag agcgatctgt
    10501 tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc acagctgcat cagcaagatc
    10561 gtaaatggcc ccgactacct acttatttcg ccattggacg caccaccgcg ctggcactac
    10621 ataccgtaag cggacagaag attctctacc cacaggatcg ggaaatcagc gaagtcttgc
    10681 tacaattacc tgaattacaa aatattgcgg gcaaatgtgc gctgatatta cgtggcaatg
    10741 gcggtcgtga gctaattggg gattccctga cggcgcgcgg tgctgaggtc actttttgtg
    10801 aatgttatca acgatgcgca atccattacg atggtgcaga agaagcgatg cgctggcaat
    10861 cccgcgaggt gacgacggtc gttgttacca gcggtgaaat gttgcagcaa ctctggtcgc
    10921 tgatcccaca atggtatcgt gagcactggt tactacgctg tcgactattg gtcgtcagtg
    10981 agcgtttggc gaaactcgcc cgggaactgg gctggcaaga cattaaggtc gccgataacg
    11041 ctgacaacga tgcgctttta cgggcattac aataactctc ataacaggaa gccataatga
    11101 cggaacaaga aaaaacctcc gccgtggttg aagagaccag ggaggccgtg gacaccacgt
    11161 cacaacctgt cgcaacagaa aaaaagagta agaacaatac cgcattgatt ctcagcgcgg
    11221 tggctatcgc tattgctctg gcggcgggcg tcggtttgta tggctggggt aaacaacagg
    11281 ccgtcaatca gactgccacc agcgatgccc tggctaacca acttacggca ctgcaaaaag
    11341 cccaggagag ccaaaaagcc gagctggaag gcattatcaa gcaacaagct gtacagcttg
    11401 agcaggcgaa tcgtcagcaa gaaacgctgg caaaacagct ggatgaagtc caacaaaagg
    11461 tcgccaccat ttccggcagc gatgctaaaa cctggctgct ggcccaggcc gattttctgg
    11521 tgaaactcgc cggacggaag ctgtggagcg atcaggacgt cacgaccgct gcagcgttgc
    11581 tgaaaagtgc agacgccagc ctggcggata tgaatgaccc gagtctgatt accgttcgcc
    11641 gggcaattac cgatgatatc gccagccttt ctgcggtatc gcaggtggat tatgacggta
    11701 tcatccttaa gcttaatcag ctttcaaatc aggtagataa tctgcgtctg gccgataatg
    11761 acagcgatgg ttcgccgatg gattccgacg gtgaagagct ttccagttcc atcagcgaat
    11821 ggcgtatcaa tctgcaaaaa agctggcaga attttatgga caacttcatt acgattcgcc
    11881 gtcgtgatga cactgccgta ccgctgttag cgccaaatca ggatatctat ctgcgcgaaa
    11941 atatccgctc tcgcctgctg gtcgcagcac aagctgtacc gcgtcaccag gaagagactt
    12001 atcgccaggc gctggagaac gtctccacct gggtacgtgc ttactacgat actgatgatg
    12061 ccaccaccaa agcgttcctc gacgaggtgg accagttaag ccagcaaaat atctcgatgg
    12121 atcttccgga aaccctgcaa agccaggcga tgctggaaaa attgatgcag acccgcgtgc
    12181 gtaacctgct ggcacaaccg gcagcaggga caacggaagc taaacctgca cctgcacctg
    12241 cacctgcacc tgcaccgcaa gctgatgctc cggcagccgc gccgcaagga gaataaccat
    12301 gctaaaagtg ttattgctct ttgtgttgct gattgcgggg atcgtggttg gcccgatgat
    12361 tgccggccat cagggttacg tgctgatcca gaccgacaac tacaatattg aaactagcgt
    12421 cacgggcctc gcgattattt tgattctggc gatggtagtg ctgtttgcca ttgagtggct
    12481 actgcggcga atcttccgca caggcgcgca cacccgtggg tggtttgtcg gacgtaagcg
    12541 tcgccgtgca cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag gcgattatca
    12601 gcaagttgaa aagctgatgg cgaaaaatgc cgatcacgct gaacaaccgg tggtgaacta
    12661 tctactggca gccgaagccg cgcaacaacg tggtgatgaa gcacgcgcca accaacatct
    12721 ggaacgcgca gcggagctgg ccggcaatga caccattccg gtagaaatca cccgtgtacg
    12781 tctgcaactg gcccgtaatg aaaaccatgc agcacgtcac ggcgttgata agctgctgga
    12841 agttacgcca cgccatccgg aagtattacg tctggcggaa caggcgtata tccgcacagg
    12901 ggcatggagt tcgctgctgg atatcatccc atcaatggcg aaagcccatg ttggtgatga
    12961 agaacatcgt gcaatgctgg aacaacaggc atggattggc ctgatggatc aggcgcgtgc
    13021 cgataacggt agcgaaggtt tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca
    13081 tcaggtggcc ttgcaggtgg caatggcgga acatcttatt gagtgtgacg atcatgatac
    13141 tgcccagcaa attatcatcg acggcctgaa acgccagtat gacgatcgcc tgctgctgcc
    13201 gatcccgcgt ctgaaaacca ataatccgga acagctggaa aaagtgctgc gccaacagat
    13261 caaaaacgtg ggcgatcgcc cgctgttgtg gagcacactg ggtcagtcgc tgatgaaaca
    13321 cggagaatgg caggaagcat cgctcgcctt ccgcgcagcg ctgaaacaac gtccggacgc
    13381 ctacgattac gcatggcttg ccgacgcgct ggacagactg cacaagccgg aagaagccgc
    13441 agcgatgcgt cgcgacggtt tgatgctaac gttgcaaaac aacccaccgc agtaatctat
    13501 atcgcctcgt tcaggggcga tattttttta caaggatgtc aaacatgcgc acaccgtccc
    13561 gctatatttt ccgcttacca tcacatgaaa ttaatccttt tcgggcaacg ctcttgctga
    13621 ttttgttgat atgtgccgtt ctggctggcg taagttggct gattctgtcc ttcgtacgta
    13681 caggcaatac attcatcttc tggttaacac tttttattgg gtatctgata gccattgcga
    13741 agcaggaaaa aatcaagctc atcgagaagc gccagattat ggctgataaa cgtcagggac
    13801 tttccatttg tcagttcgca cgccaatttt ccccccacac cgtagataca tgggtgattc
    13861 gggctgtgtg gaatacctta caaggaaacg gctatatcga ttatccatta ccactgaaag
    13921 ccagtgataa actggacgat gatctggatt tagtaaacga tgcagacgag ttagaagagc
    13981 tggtcgaaga tattgccgct cgttgtgggc gcgatctacg cggaatagaa gataatccgt
    14041 ttcttcccat caccacggta ggttctcttg taagtgtatt aaacgcacaa ccgatgaccc
    14101 aagagcgacg tagcctgttg tttactcggt cataaaaaaa cgcctgctct tattacggag
    14161 caggcgttaa aacaggtctg tatgacaaca agtgggtgct tcactcaacg ttgtgtccat
    14221 ggtgtctgat gaggcagaag cgacatctgt cagtggacga taagcaccgt aaacggctct
    14281 gcgtcattcc ggagtttatg aggcactaag gcgaacataa gagatggaat gagcatctac
    14341 tcgtttatta tgccacagag aaccgggaaa taacatccct taacacttgt tatgagataa
    14401 ttctgtaatc ctctttgctt cctgagtaat aacttcctga gtgaatattt ctcctgcgca
    14461 caattctaca catatatata atgaataata aaagtcattc aattaataac atatatatta
    14521 attgcagata aaactaaaaa cagcatcaat aatcaacgcg atataacatc accgccttac
    14581 atatcacctg cgccagaggt aggattgaaa acgctctcct gattttccaa ttcattttct
    14641 ggataaataa ataatttatt tttatcacca ttatttatgc aatcatcctg tcagggagag
    14701 ggatctcaat tatcaatgct taattacgtc atcattttga cgacatgaaa ccggagcatt
    14761 ttgcatggaa ttttcgtttt cacccaaact tcttgttgtt gctgtcgccg cagctcttcc
    14821 tctcatagcc agcgcagcag ataccccgtc aaccgccacc gcacgcaaag gctttgccgg
    14881 atacgatcac ccaaaccagt atctggttaa accggcgacc actattgccg acaacatgat
    14941 gccggtgatg cagcatcctg cacaagacaa agaaacccag cagaaactgg cggaacttga
    15001 gaaaaaaacc ggtaagaaac cgaacgtggt tgttttcttg ctggatgatg tgggctggat
    15061 ggacgttggc tttaacggcg gcggcgtggc ggtgggtaac cctacaccag atatcgacgc
    15121 cgttgccagc caggggctga ttttaacttc ggcgtattct caaccgagtt cttccccaac
    15181 ccgcgccacg attctcaccg gacaatactc catccaccac ggcattctga tgccgccaat
    15241 gtacgggcaa ccgggcggtc tgcaagggtt aaccacgctg ccacagttgc tgcacgatca
    15301 gggctacgtc acccaggcta tcgggaaatg gcatatgggg gaaaacaaag agtcgcagcc
    15361 gcagaacgtt ggctttgatg atttccgcgg ctttaactcg gtatctgata tgtataccga
    15421 atggcgtgat gttcatgtca acccggaagt cgccctgagc ccggatcgtt ctgaatacat
    15481 ccaaaaactg ccgtttagca aagatgacgt tcatgccgtg cgcggcggtg aacaggaagc
    15541 cattgccgac attacgccga aatatatgga agatctggat caacgctgga tggaatatgg
    15601 cgttaagttc ctcgacaaga tggcgaagag tgataagcct ttcttcctct attacggcac
    15661 tcgcggctgt cacttcgata actacccgaa cgccaaatat gcgggtagct ctccggcgcg
    15721 cacctcttac ggcgactgca tggtggagat gaacgatatc ttcgccaatc tgtataaagc
    15781 actggagaaa aacggtcagc ttgataacac gctgattgtg ttcacctctg ataacggtcc
    15841 ggaagccgaa gttccgccgc acggtcgcac gccgttccgc ggtgcgaaag gttctacatg
    15901 ggaaggcggc gttcgtgtgc caaccttcgt ttactggaaa ggcatgatcc aaccgcgtaa
    15961 atctgacggc attgtcgatc tggcggatct cttccctacg gcgctggatc tggcggggca
    16021 tcctggggcg aaagtagcga atttagtacc gaaaaccacc tttatcgatg gtgtggacca
    16081 gacatctttc ttcctgggaa caaatggtca gtctaaccgt aaggccgagc attacttcct
    16141 caacggtaaa ctttctgctg tgcgtatgga tgagttcaag tatcacgtcc tgattcagca
    16201 accttacgct tatacccaga gcggatatca gggtggattc accggcacag taatgcaaac
    16261 ggcgggatcg tcggtgttta acctgtacac cgatccgcag gaaagcgact ctatcggcgt
    16321 gcgccatatc ccaatgggtg taccgctaca gaccgaaatg catgcgtata tggagatcct
    16381 gaaaaaatat ccaccacgcg cgcagattaa atctgattaa gccggcgcta tcgctgagat
    16441 ctacctttgc cggatgcgat gctgacgcat ccgacaatca accgcaggcg gccacaaaat
    16501 ggctgctttt ttacttattc accaccagca aatgcgcctg cataatgtcg ctggccggac
    16561 gaccgtgcgc cagcaaatca accattgctt taagatatgg cggtagatgg cggaaataac
    16621 gctgataccc ggcacacaaa taattcagct cgggtttgcc gctggcatcg agcataaagc
    16681 ggtgtttcgg gcagcctccc cagcacgctt ttaacacgtt gcaactgcga cactgtgccg
    16741 gtaactgctt gaatttatct tcaccaaatg cctgctgttg cggggaatca atcatttctg
    16801 caatcatctg ctgaagcata ttccctagtc gatactgcgg ataaacatag tgatcgcagg
    16861 cataaacgtc accgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc
    16921 aaaccgcgcc cggtgcgccg acaaaattgg cgaacgccca ttcgatattc attacgaaaa
    16981 tcttgccgac gtcgcgctta atccagtggt cgaaaatcgc caccagaaac tcaccgaact
    17041 cttgcgggca caccgaccat tccgtcagtt cgccctgaat atcgccggga gcatgtagct
    17101 taagtccagc atgagcagcc gtttcattag ccaggcgctc gaccaccgga ataaactgga
    17161 tgaactcaac acctgcatca cacagaaaat catagacttg caaaggttgt aacgcactgg
    17221 tgcgattgac gcagaccagc acgttatagt cgacatgatg tttttgcagg agcgtcaggg
    17281 cacgcatcac cagcttatgc gtgggtctgc cacctttggt cacgcgatat tgattgtgga
    17341 tctcagccgg accatccagc gataacccta caagaaaatg attttctgcc agaaatccac
    17401 accattcgtc atcgagcagc acgccgttag tctggaagct attacttatc ttcctgccag
    17461 caccatattt cgcctgtagc gccacggcac ggcggtaaaa ctccagcccg agtaacgttg
    17521 gttcgccgcc ctgccaggta aaagccactt cgttttgggt ttcgctggca gcgatatagt
    17581 gacggacata cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt tcgtggtaaa
    17641 gggattgttt ttcgagataa aaacagtagt cacagttcag attacaatca gaaccgctcg
    17701 gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg ctgcatggtc gctccttgcc
    17761 tgaagtcatc cttcaataag ttaagggcga aatcataccg tggcgcaatg cgttattacg
    17821 ggcgtttatt gcgaagggaa agtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc
    17881 ggggttcaaa attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca
    17941 gttgcgctac caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt
    18001 ctttaaagtc gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca
    18061 cctgaagcta gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg
    18121 gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac
    18181 tgagctatag ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc
    18241 gccgggacta aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc
    18301 agcgctctat ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga
    18361 cttcccaccc ctctgtctag tcccttttta aataaattgc gcgtttggtt acggtttgtg
    18421 cgttttgctg cttattcctc cagtttatgc gctttaccgt ggcgattaag cccaaaaact
    18481 ttataaatcg ccgtcaccgt cagcataaag atgatgccga caaacagcga catacgcgta
    18541 tcttcattaa agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg
    18601 gcccaaggga acaggattga gcggaacgga tggctggcaa ttgccgcttt atgcgcacga
    18661 cgaaaacgca gctggctaat caatatcaca aaccatggca ccatccccgg aagcacgctg
    18721 gcactgtaga catagacaaa cacacgctgc ggattgggaa tgatgtagtt caggcatgag
    18781 ccaattagca gaatagcaat agataccgcc acacccgcaa ccggtacgcc gtgacgggaa
    18841 actttcgcca tcgccgccgg taactgacgg tttttcgcca gtgcatagag catacgtccg
    18901 caactgtaca tgccactgtt acagccagaa agcgctgccg tcagcaccac aaagttaata
    18961 atgcccgccg cagcggtaat gccgattttg gcgaaagtca gtacgaacgg gctgccgttg
    19021 ctgcctattt cattccacgg gaagatggta acgataacga aaatcgcgcc tacgtagaaa
    19081 atcaggattc gccacagcac cttgcctaca gcgctacgca gcgtcacctg cggattcttc
    19141 gcttcaccgg cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac
    19201 aatgcggtca ggaacccttt ccagccaccc gcaaagaaac cgccatgctc tgtgagattg
    19261 ctaaaaccaa tcgactgccc gccattgcca aagccaaaga aaatcacgcc caggccaatg
    19321 acaatcatca cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg
    19381 tacaacctca ccgcagccag attcgccaac gccaccagcg ccaccgcgat caatgcgggt
    19441 atccactgcg ccatctccgg gaaccagaac tggacataaa cgccaatggc ggtgatttct
    19501 gagatcccca ccgccatcca cataaaccag taagaccagg cggtgagata gccaaagaac
    19561 gggctcatat aacgatgcgc gtaaacggcg aacgaaccgg taaccggttc gaggaacaac
    19621 atttcgccca ttgaacgcat gatgaaaaag acgaacagcc cggcgatgat ataggccaac
    19681 aatacggatg gcccggccca tttcagggta ctggcggccc ccataaacag gccgacgcca
    19741 atggtgcccc ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct
    19801 ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt
    19861 atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata
    19921 aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac ggagtgacca
    19981 cttcgtcgct ttacaaagag aggaaaatca gaggttgccg gtatagtgcc agcgtaaata
    20041 acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca
    20101 ctccagcccc agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg tgaaaacatc
    20161 gtaggtcccg ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat
    20221 gaagatctcc tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg
    20281 aatgcgttca aacagcgcct gacgctgctc gggtttaaaa taaccatcct ggctgccaac
    20341 gatattcaca ttccactggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt
    20401 accgcccaca agaaataccg gtgtcccttc tttgcctgcg cgcgccatca gctcttccca
    20461 gagatcggcc ccagcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg
    20521 tacaacgctt atgccatccg catatttaaa ttcggcagcg ttaattagct cccggacctc
    20581 ggcgttatct tcaatggtca gcattttttc ggcattaatg gcaaccagcg ttccttgctt
    20641 aagctgcccg tcagcgaaca gataatcgag ggcgtgctgc atatcacgcc aaccaatcaa
    20701 ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg ttattcatta ttatccttca
    20761 acctgcgtcc ggagcgatga ttttgttcgt ttatgaatga gtccggcgct ttcaaaaagc
    20821 cagtacaaca gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagaccacg
    20881 cgtgagacaa acgaatccag cccttcacgc gccagcacga tcatattgaa gatcgccccg
    20941 aagcagaaac tgtgcaatat cgcagcctta tagcgattag tctcgcggtt gcccagctca
    21001 tacagccagt cgaaccattt gatgatcagt ccaacaacaa tcgccccgag cgggatgaac
    21061 aacgcgccgc ccatcaccac cagtgagcct ataagcgtag gcgagatcgc cagtccggag
    21121 tggttattca gcacttccca ggtaaagtag ttggcggagt tcagcaccat actcgggcga
    21181 cccggccaca gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc
    21241 tggaagtcga tgttgtcgta gttctgcaac agcaacgcca gattctccca cggtgaaaag
    21301 gtgtcgcgag tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca
    21361 tagcgtttta acgccagcca gaacatgccc acaatcccca gcacgcccgc cgccgccagc
    21421 atccacaacg aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg
    21481 atgatattgg cgcgagtgcc gccgacaatc atataagtca gcaagccaaa agcgaccgtg
    21541 ctgacgagga aaaacagcca cgctttgctg tcctggcgca gaaagtagac caccagcatc
    21601 gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc actggagaag
    21661 atctgactgt aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg
    21721 acgcttacca gcgcgatgcc catcaggatc acccacgtga gattggtttc cacgcggttc
    21781 atggtaaaca gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg ggttttgtag
    21841 gtgacgtagt aaaccgcgta gaagcagccc gcagaaagca acgcctgcaa caagatttct
    21901 ggcggcgcgg caccaacatc aaagcgaaaa accagcacgc tggtcagcgg gaagccgaag
    21961 aaaaaggtga gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga
    22021 aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga gcagccagac aacaaacagg
    22081 ccactgaatt gcagcagact catgcgacct ccccggcggc aatcgccaac gcccgctgcc
    22141 agccttgcag atagttcggg ctaaagaagg caatggtgtt tttatccacc gacaccaact
    22201 ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc ggtagtaaac agcaccggca
    22261 aatgctgttc cgtcatatcc tgccagaacg gattttcccg gttaagcaca caaggaatgc
    22321 ccgcctgaat cagtaagcac agcgtaccaa tgccctgctg gcgggcaaaa ataaagtaac
    22381 caagatcgca ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt tcgctcagag
    22441 tttgtagatt ttcttcgctg aataactcca gccccgcctg acgaacttcc tcgatgtacg
    22501 cttcgttatt aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct
    22561 gataaacggc gcgcaaggca gcaatatgtt cattgctgcg gtcaccggag ttccccacca
    22621 gaatggtcat tttcccttca cgttgccgat cattcgccat catattgagt gaagggtcca
    22681 tccgcgtcgg gaagtacagc agttctcccc gcacctttgg atgcgttttg gcaaaaaagc
    22741 tcaaatcacc gcgggtggca aatacacagc cgactctctt ttgcgccagg cgacgtagcg
    22801 ggtaaaaaag cttatatctc aagccactgg aaagctcgta caggtctgcc ccccagatat
    22861 gccagtaaaa ctggctgggc ttaatgccac cactcagcag agccagccac agtgtgggat
    22921 tgaactgacc gtggaagaaa aaacgctgct gacggttagc ttttgctttc gcgatgaccg
    22981 cttccgccag cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac
    23041 ttaagccgtc gtccttgcca acaaccataa actcgcgcgc gtgctcgctc gtcgcggcca
    23101 gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg agggatatcc gatcccagta
    23161 cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga
    23221 aaaatagacg atataggttg ccatataggc ctgcgccgcg cccagtgcac catgcgcagg
    23281 gattagccag tgggcaaata ccatcaataa agtgaactgg ctgacttccg ccagaatata
    23341 aaaccgcagt gacgctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa
    23401 cacatcaccc accaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa
    23461 cagcagccag atagcaaaat cacgcagcaa ccagacggta aaactcgccg ccgccactgc
    23521 cggcaagacg aatttaagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag
    23581 ccgcgacaac gtgggcagca aatatacgct gaacgatgcc gtaataaatt gcaggtaggc
    23641 atcggaaata ctgctcaccc cttgccagat ccccacctca tcccagctat actgcgccgc
    23701 cagcagttta cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa tcaacgccat
    23761 gagcgtaaat ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct
    23821 taacgggatg acaccacgtt tgatcaacat gatggcggca ggaattacca ccagcgcggg
    23881 aatcagcgcc agacccagca acgccccttc ataaccgccc aaacggtaac tgacgtagta
    23941 cgcgagaacg ccaatcaagc tgccaacaat cagagataac gcattacctg cggcatcgcg
    24001 aaagcctttc atcagcgcca gtaacaggtt gccccaggcg atccccattt gcaccagcgc
    24061 cactaaacgc accagcccct gatagtcggt attaccaaac aatccctggc tgattggcgc
    24121 agctgccagc acaaaaacca gcgccatcag cgtagagaaa ccaagtacca tcgctgatga
    24181 agtgccgacc acgcggcgca gctgttgcgg attatcatgg tactgggcaa cgtatttggt
    24241 tacaccgtta aagatgccag ccccggcaag cacgccgagc acggtaatca actggcggaa
    24301 atttgccgcc agcccaagcc ccgccggacc aaatgacacc gccagcaact taccgactag
    24361 taacccggca ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa
    24421 cgacatatca ggagaagtag ttcaacaaag tcgcaattac cgtacgctga ttgacgggcg
    24481 acaggttgta gaacagcggc aggcgcagca ggcgctcgct ctctttggtg gtgtagcgat
    24541 cttcaccgtg gaactcacca aagtgttccc ccgcagggca accgtgcagc ggaatgtaat
    24601 gaaacaccgc catgatttcc gcttctttca gaaagttaat caacgcgctc cggtcatcaa
    24661 tatcccgcag tttaatgtag aacatatgcg cgttctgcac gcagccatcg ggaatcgacg
    24721 gcagctcgat acgcccattt tttgccagag gcgccagtgc atcgtagtag ttttgccaca
    24781 gcgccagacg ctgctgattg atacgatccg ctgcttccag ttgcgcccac aggtacgctg
    24841 cttgcagatc ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct
    24901 gaccacggaa gaactggctg cggtttgtgc ctttttcacg gatgatctcg gctcgttcga
    24961 tcaacgcttt atcgttaatc agcgtcgcgc cgccttcacc acccgccgtg tagtttttgg
    25021 tttcatggaa gctaaagcag ccaatatgac caatggttcc cagcgcacgc cctttgtaag
    25081 tggacatcac gccctgagcg gcatcttcca ccacaaatag attatgcttc ttcgccaacg
    25141 ccataatagt gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg
    25201 ttttgtcggt gatcgccgct tcaatcagcg tttcgttgat gttcatggtg tccgggcgaa
    25261 catccacaaa gacgattttc gcgccacgca gcacaaaggc attggcggtg gagacaaagg
    25321 tgtagctcgg catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct
    25381 ccagcgaagc ggtgcaggac ggcgtcagta acactttggc gctgccaaaa cgttgctcca
    25441 gccactgctg gcagcgacgg gtaaaaccac catcgccgca cagtttgccg ctacccattg
    25501 ccgactgcat atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt
    25561 gatcacctgt ataaccagta cgcggtgctt tctacattcg caccactttg tatgtatcgt
    25621 ttaagcgcgg cggtgttgcc catctgggtc gccacccgca aagttgtttt accgcgagca
    25681 tacgcccagt ttagcgccgt ttgcattagc tcagcacctg caccgcgtcc agccagcagg
    25741 ccaattcgcg catctgtcgc attgagttcc cgtaaagaga cataaccgcg aatatcgccg
    25801 gacgccgcac gtaaaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc
    25861 cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc acggcgcacg aaaacggctt
    25921 tgcgcaaatg cggcgctggc taactgacgt aatgcgggaa tatcggtctc ttgtgccact
    25981 actgcaccgc tatcactgac attgttcacg ggtagtgcca aatcaacttc accttctatc
    26041 agggagaatc ccagctgttg cagagcatcc agttcacccg tatttgatgc cgcaattttg
    26101 gcctgcaccc gtgaccacgg cgctaaggcg tctggcgtca ggagtgccgc gtcagggcta
    26161 atacgcacaa tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata
    26221 ctggcgcgga cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgctaa
    26281 tgaactggca gcacgcttca caccctcgtc atcgagccag ccgttacgcc aggcaatctc
    26341 ttccaggcag gcaatcttaa agccctggcg tttttctact gtctgcacaa aagtgctggc
    26401 ctcaatcagg ctgtcgtgag tgccggtatc cagccaggca aatccgcgcc cgagcagttc
    26461 aacggtcagg ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc
    26521 acgctccgac ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag
    26581 cccggtcacc gcccagtttg atttcggctg ttttggtttt tcttccagcg agatagcgcg
    26641 gaaattgtcg tcaaactcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa
    26701 taccgtcgcc ccttccgtgc gcgccgcaac atgacgcagt ttcggactga agccctgtcc
    26761 gaagaagatg ttatcgccca gcaccagaca agaaggttca ccgttgagga aggtttcacc
    26821 gatgataaag gcctgcgcca ggccgtccgg actgggctgt tcggcatatt ccagctggat
    26881 accgaactca ctgccgtcac ccagcaggcg ctggaaataa cctttatctt ccggcgtagt
    26941 gatgatgaga atttcgcgga taccggccag catcagcacc gacagcggat agtaaatcat
    27001 tggcttatcg taaatcggca acagttgctt agatacgccg cgcgtaatcg gatgcaatcg
    27061 ggtgccggaa ccgcccgcca ggataatacc tttcatgttt gcctccgctg aaaattagcc
    27121 tttcagacct aaacgctcgc cctgatagct gccgtcctgc acctgcttcc accagctttc
    27181 attagccaga taccactgca ccgttttacg cattccactt tcaaaggttt cctgcggcag
    27241 ccagccaagt tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg
    27301 acggtcagcg acaaaggtga tcaggtcacg atagtgcgcc acgccgtgcg gcttattcgg
    27361 agccagttct tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt
    27421 gtgaccacca atattatagg tttcaccgac tttcccggtg gtcgccacgc aatacagcgc
    27481 gcgggcgtga tcttcaacat acagccagtc acggatttgc tgcccgttgc catataccgg
    27541 cagcggttta cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg
    27601 gtaaggaccg tagttattcg agcagttggt gataagcgtc ggcagaccat aggtccgcag
    27661 ccaggcgcgc accagatggt cgctgctggc ttttgacgcg gaataagggc tgctcggcgc
    27721 atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc
    27781 ggtggagata tgatgaaaac ggaacgctga ttttttatct tccgtcagcg cagtccagta
    27841 agcccgcgcc gcttcaagca gtgtataagt cccgacaatg ttggtttcaa taaacgctgc
    27901 cgggccgtca atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg
    27961 ctgatactca gtgaatacgc gcgccagttc tgcccgatcg cagatatcaa ctttctcaaa
    28021 ggcaaagcgc tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg cgtaggtcag
    28081 cgtatcgacc accaccaccg cgtcgctcgt ttcgttgatg atataacgca ccagcgccga
    28141 gccaataaac ccggcaccac ctgttatcag aatctttctc atcagcgcca gactcctttg
    28201 gcatcgacga catactgctg atggacgttg tcgccattga taactttgaa ctgactatga
    28261 tcgaccagca tcaccagcac atctgccgtt gccagcgcct cgtcaagctg cgccagagta
    28321 caaagcccgg tcagtttctt cggcaactgg tggatgttag gctcaacaac cagcgtttcg
    28381 ccgctgtgcc actgggcgat cagttcagcg atttccattg ccggactttc gcgcaggtca
    28441 tcaatattcg gtttaaacgc cagaccaaag caggcgattt tcagttcatt ggcgcgttta
    28501 tcggtagcag ccagacaatc agccaccgcc gctttcacct gatcgataac ccagaacggt
    28561 ttgtgatcgt tcacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc
    28621 acgataaacc acggatcaac agcgatgcag tgaccgccca cgccagggcc tggctgaaga
    28681 atattgacgc gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga
    28741 tcggcacaaa tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt
    28801 tcggtgagct tacacatttc cgccgtccgc gagttagtga cgacacactc accttcgagg
    28861 aaaattttgt acagttcgct ggcgcgggcc gaacaaaccg gcgtcatacc accaatcacg
    28921 cgatcgtttt taatcagctc gaccataacc tggcccggta atacgcgttc cgggcagtaa
    28981 gcaatgttga cgtccgccaa ctcgcccacc tgctgtggga aagtgagatc tggacgcatc
    29041 tctgctaacc attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc
    29101 actaacgcgc cttttttcag cactggcgca atggagcgag cagccgattc aacgtaggtc
    29161 atatctggct catgatcgcc tttaaacggt gtgggtacag caatcaacca ggcatccgct
    29221 tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc
    29281 gccaaatcag gctcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg
    29341 ttgatatcga caccaatcac ctgtttttgc cttgaggcaa acgcagctgc tgtaggcagc
    29401 ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat
    29461 tttttaacgc ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc
    29521 ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc
    29581 gctgcttatc cgtgccgacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag
    29641 tggtatcgcg catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc
    29701 ctgagtcggt caaaatcagc caggcgtggt tcatcagcca gacaaacggt aaatactcct
    29761 ggggatcgat cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga
    29821 cgttcgggtt gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcgatgt
    29881 ctgccagcgc atggcagatt tcttcaaagc cacgaccgaa gctctcacgc ctgtgaccgg
    29941 tcaccagaat catcttttta tcggggtcga taaacgggta atttgccgcc agttctgaac
    30001 gcagcgtatc gctgctcatc acctggtcac gcacccataa cagtgcatca atgactgtat
    30061 taccagtaat gaagattcgg ctatccgcaa cgttttcacg cagcaagttt tgccgggaag
    30121 tttcggttgg agaaaaatga tacatcgcca gatgcccggt caatgtacgg ttagcctctt
    30181 ccggccacgg cgaatagaga tcgcccgtgc gcagaccagc ctcaacgtga ccaacaggaa
    30241 tacgctgata aaacgccgcc aggctggttg ccagcgtcgt cgtagtatcg ccgtgaacca
    30301 gcacgacatc tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg
    30361 ttatctctgt aaggccttgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg
    30421 aaaagagttt cagcacctga tcgagcatct cccgatgctg cgcagtgacg caaactttag
    30481 cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg tgccatcttg atggcttccg
    30541 ggcgcgtacc aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagcg
    30601 cctttgcgct caccgcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca
    30661 gccccgatca gtcccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta
    30721 tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc
    30781 agggttggac caacattcag ggtgtttaac atggcccgat tttgatcata gtcgagatca
    30841 aaggccggac cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc
    30901 aggaacattt ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata
    30961 ttatgctgct cagcaatttt cagcgcctgt tcaatactgt tcatccggcg gtcatagatg
    31021 gctttcgcca cctcttcctg acgcttcacc tgagctttca tctggatagt gcgtgccgcc
    31081 catgcgcctt tcagctcatc attcagatgg ctggctgcac gctggctggc aaaagcaaca
    31141 tactgacgta acaggttatt agcgtcaggc gcggtttcgg caataagctt cacgctgtca
    31201 ttgaccgcgc gggtaaagtc accgggaata aacacgatgt tgttaatcat ttcatccagc
    31261 aacgccgcat cggctttgct gttgcccacc atccgctgtt tgtaatagtc ggtttgcagc
    31321 cagaactctc tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataagcttcg
    31381 tccatgaccg atggttggtc ggcagaagcc atgtttgaac ggacatccag gttacgcaaa
    31441 aattgctgct gtgagtagta cccgcccagc atattcaccg ttggacgatc ggtaatcgcc
    31501 gtcgcgctcc actcctgacg agcaaaaaaa gtatacgcca gcgcgattaa cgcaaacgcc
    31561 agccccatgc caataatcca tagcttccca gcccacaagg tacgaaacaa cccacgaata
    31621 tccagttcat tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc
    31681 tcatttattt ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc
    31741 gcttaataaa gcgagcaact ttccaggcac gcttgatgca atatccatag aggaagaatg
    31801 ctagcaaaaa gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca
    31861 cgccaatgga agcgagcaat gctgcggcaa gggtaatcag cacaaacgcc tggcgggaag
    31921 taaacccggc acgcatgatc aaatggtgaa tatgctgacg gtctggagag aatgggctca
    31981 tgcctttacg caggcgacgg tacataatcg ccaccatatc cattagcgga atggcgataa
    32041 tccacaaagc ggtaaccggg ctgatgggat gagttttgcc ctgggtcgtt tcgagcagga
    32101 tccagataac ggtaaaacca atcagcgtac tgcccgcatc ccccataaag actttgtagc
    32161 ggcgacccag gataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa
    32221 agcaccagat tgcgagactg gtttgcccgt cgaaccacaa aatcataccg attgctgcaa
    32281 acgagacgca ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat
    32341 taatggccgc ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct
    32401 cccaggagcc aaagatataa cccaggctac tgagataaag tttgccgaac accatcataa
    32461 caatgccaac agcggcctgt atggtggctc ggatttttac gctgatatca aaacggtcat
    32521 ccagcgcgcc aatgaaaaca agcacaccag cacaagcgag atagagagat gcatgcggaa
    32581 tatagtagtc gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa
    32641 cgagaggtat caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt
    32701 tttttgccac cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga
    32761 gatcagtact cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga
    32821 agtataacca cgaagacctt tattcagaaa ggaaagtccg ttccgaacct accagcacta
    32881 tgcgaaaatt aaccacttga tatgcaaatt gcataacaca ccagcaaatt ccaggaattt
    32941 tcctaacatc gccagcgcgg cattaagatt acagatataa aagcaaaacg ccacgtaaac
    33001 acgtggcgtt tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc
    33061 attggtcttg gtcattgcca gtttattaat gaggaattcc attgcatcga tttcgcccat
    33121 cgggtgaatg attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag
    33181 ctcttcttta cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc
    33241 gatcttacga gagaggtgca gttccatgtt gcctgtacct ttaaactctt cgtagataac
    33301 ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc
    33361 ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga cgatgcaggg cgttggcgtc
    33421 cacaccaccg gtcaacactt tacctgacgc cggaacaacg gtgttgtaag cgcgcgccag
    33481 acgagtgatg gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttcgc
    33541 cttctcgatc accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga
    33601 agcaacaact tcacctttta ccagtcgctg catctcggtt acttcttccg gacgttcgtc
    33661 gatcagcaga accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt
    33721 ctgcagcagc atggttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg
    33781 accgataggt gatgccagat ccagtacgcg agcggttaaa tcttccgtag aaccgttacc
    33841 acgttccata cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt
    33901 gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata
    33961 gcgttcacct tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt
    34021 gaaacggcgg atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct
    34081 gtctgcggaa cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa
    34141 gatatcttcg ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg
    34201 catacgagcc aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg
    34261 cgtattcttt aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct
    34321 cgaacttaat gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact
    34381 catgtctttt cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt
    34441 gagtgacgag ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa
    34501 ttacaagatt caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga
    34561 ctcacgccgg gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc
    34621 aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcgcccac tttggttgcc
    34681 gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc
    34741 gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg
    34801 tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag
    34861 aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc
    34921 aggtgaataa ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt
    34981 cgttgttggt gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg
    35041 taaagcaata gtaagctgat attctaccac actatgagca aaacacattt aacagaacag
    35101 aagttttccg acttcgccct gcatccgaag gttgtagaag cccttgaaaa aaaagggttt
    35161 cataactgta cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta
    35221 gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat
    35281 tatcttctct ctcatcccgc gattgctgat cgcaaggtga atcagccgcg tgccttaatt
    35341 atggcaccga cgcgtgaact tgccgtgcag atccacgccg acgcggaacc gctggcggaa
    35401 gctaccggcc tgaagctggg tctggcttac ggtggtgatg gctacgacaa acagctgaaa
    35461 gtgctggaaa gcggcgttga cattctgatt ggcaccacgg gtcgtttaat tgactacgcc
    35521 aagcagaacc acattaacct cggtgccatt caggtggtgg tgctggacga agccgatcgc
    35581 atgtacgatc tgggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca
    35641 aaccagcgcc tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg
    35701 ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac
    35761 cgtattaaag aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg
    35821 ctgatcgaag aagagtggcc agacagagcg attattttcg ccaacaccaa acaccgttgt
    35881 gaagagatct ggggccacct ggcggcagat ggtcatcgtg tcggtttatt gacaggcgat
    35941 gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta cccgtggcga tctggatatt
    36001 ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc ctgctgtgac acacgtcttt
    36061 aactacgatt tacccgatga ctgtgaagat tacgttcacc gtattggtcg tacaggtcgc
    36121 gctggcgcaa gcggtcattc catcagcctg gcgtgtgaag agtatgcatt gaatttgcct
    36181 gctattgaga cctatattgg tcactcaatt ccggtaagta aatacaatcc ggacgcattg
    36241 atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc gcacaggcaa tggtccgcgt
    36301 cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa gaaaaaatgt catactccaa
    36361 atcattcaag ctgtatcaag atggcaagag aatgaatccc gatgagttta ctgtcgtaag
    36421 tgattcaggt gaaagaatgc agccaacaca gagacagatt gaaggatgaa gagtatgggt
    36481 tccacctcgt cgctgtatgc agccattgat ctcggttcga atagttttca tatgctggtt
    36541 gtgcgcgagg tggctggaag catccagacg ctgacgcgaa taaaacgcaa agtgcgtctg
    36601 gctgctggcc tgaatagcga aaatgccctg tctaatgaag caatggagcg cggttggcaa
    36661 tgtctgcgcc tgtttgctga acgtctgcaa gatatccctc ccccgcaaat tcgcgttgtc
    36721 gctacggcga cattacgcct tgccgtcaat gcgggtgatt ttattgccaa agcgcaggaa
    36781 atcctcggtt gtccggtaca ggtgatcagc ggtgaagagg aagcgcgtct gatttatcag
    36841 ggcgttgctc acaccactgg cggtgccgat cagcgcctgg tggtggatat cggcggagcc
    36901 agtactgaac tggtaaccgg cacgggtgca caaaccacct ctttgttcag cctgtcgatg
    36961 ggctgcgtta cctggctgga acgctatttt gccgatcgta atctggggca ggaaaatttt
    37021 gatgctgcag aaaaagcggc acgcgaagtg ttacgtccgg ttgccgatga attacggtat
    37081 cacggctgga aagtgtgcgt tggcgcttcc ggcaccgtgc aggcgttaca ggaaatcatg
    37141 atggcacagg ggatggatga acgcattacc ctggaaaagt tgcagcaact gaaacaacgc
    37201 gccattcatt gcggtcggct ggaagagcta gagattgacg ggctgacgct ggaacgtgcg
    37261 ttagtgttcc cgagtggtct ggcgatcctg atagccattt ttaccgaact gaatattcag
    37321 tgtatgaccc tggcgggcgg tgcgctgcgt gaaggcctgg tctacggtat gttgcatctt
    37381 accgtcgagc aggatattcg cagccgtacg ctgcgtaata ttcagcgccg ctttatgatc
    37441 gatattgatc aggcacagcg cgtagccaaa gttgcggcta acttcttcga acaggtggaa
    37501 aatgaatggc atcttgaagc aataagccgc gatttgctca tcagcgcctg tcagcttcat
    37561 gaaatcggcc tgagcgttga cttcaaacaa gcgccgcaac acgctgctta tctggtgcgt
    37621 aacctggatc ttcccggttt tacccccgca cagaaaaaac tgctggcgac gctactgctc
    37681 aaccagacta atccggtcga tctctcatcg ctgcatcagc aaaatgccgt accaccgcgc
    37741 gtcgcagaac aactctgccg tttactccgc ctggctatca tttttgccaa ccgtcgccgt
    37801 gacgatctcg tgccagagat gacattacag gctaaccatg aactgttgac cttgacgctt
    37861 ccgcaaggtt ggctaaccca acatccgctg ggtaaagaga ttattgatca ggaaagccag
    37921 tggcagagct atgtccactg gccgctggaa gtgcattaat gagtaagtgc cggatgcgat
    37981 gctgacacat tttttccggc cttgattatt tccctcgttt tgccgccatc atcgctttca
    38041 gattcgccag atggctttgc cctttctgca tccgttcttc ggcgctgacc actttgcgct
    38101 cctgttccca aatcaaatca tcctgcggca gctccagcag gaagcggctc ggctccgggc
    38161 gcaccagttc gccgtactga cggcgttctt tacacagcgt aaaggtcaat tccttctggg
    38221 cgcgggtaat gccgacatag gccagccgcc gctcctcatc aatattgtct tcatcgatgc
    38281 tgctctggtg gggcaaaaac ccttcttcca taccaaccat gtagacatag ggaaactcca
    38341 gccctttcga cgcgtgcaga gtcatcagtt gcacctgatc cagttcttcc tcgctctcgc
    38401 cgcgctccat catgtcgcgc agagtaaagc gcgtcaccac ctgggtgagc gtcatcggct
    38461 catccagttc gctgccttcc agcatctccg tcatccagct aaacagttgg ttgacgttct
    38521 tcatgcgcat ttcggcagct ttcgggctgg gcgatgtttc gtacagccag gattcataat
    38581 ccatgccgtg gatcagatcg cgcaccgcgg caatcggctc ccgctccgcc agacgctgga
    38641 tttctgccag ccagtgagtg aagcgggtca atgcttcata accacgtccg ctaagcgtct
    38701 gactcaggcc catatcaaag ctggcggtaa acatgctttt attgcgcgtc atcgcccatt
    38761 cacctagctt tttcagcgtc gccgggccaa tctctcgctt cggcgtgtta acgatacgca
    38821 gaaatgcgct gtcatcgtcc gggttagtca gcacgcgcag ataagccagc aagtccttga
    38881 tttcaggacg agagaaaaac gacgtaccgc cagatatttt gtacgggatg cggttttgca
    38941 tcaggaattt ttcaaatacc cgcgactgat ggttgccgcg ataaagaatg gcgtaatctt
    39001 tgtactgcgt tttattgacg aagtgatggg cgatcagctc gccggtgacg cgctcggctt
    39061 catgttcctc gttattcgcg cttaaaactt ttagctctgt gccgtaaccc agttcggaga
    39121 acagacgctt ttcaaagacg tgcgggttat tggcgatcag gatgttcgcc gctttcaaaa
    39181 tacgtccgga agagcggtag ttctgctcaa gcttaatcac cttcagcgcc ggaaaatcct
    39241 ggctcagcag caccagattc tgcggacgtg cgccgcgcca ggagtagatc gactggtcat
    39301 cgtcacccac cacggtaaag cgcgcgcggc tgcccaccag cagtttcacc agttcatact
    39361 ggctggtgtt ggtgtcctga tactcatcca ccagcaggta gcgaattttg ttctgccagc
    39421 gctcgcggac ttcttcattg cgttgcagca gcaacgtcgg caataaaatc agatcatcga
    39481 agtcgagaac gttacaggct ttcaggtgtg catcatacag cccataacaa tgggcaaaaa
    39541 tacggtcccg ctcgcccttt gcctctgccg ccgcctgcgc cggtgttttg agatcgttct
    39601 tccagttgga gatggtcgaa attagctgtt gcaggagaac tttgtcatct tcaatcagcc
    39661 cctcggtcaa ctctttaagc aaagcaagct gatcggtatc gtcaaacagc gaaaagttag
    39721 ccttcatccc cagcgccgca tactcgcgtt tgatgatatc cagccccagc gtgtggaagg
    39781 tggaaatcat cagcccacgc gcctctttgc gccccagcgt ctgcccgaca cgctctttca
    39841 tctcgcgtgc cgccttattg gtaaaggtca cagccgcgat atgtcgcgcc tgataaccgc
    39901 aaccgcggat cagatgggcg attttattgg tgataacacg agttttaccg gaacccgcgc
    39961 ccgccagcac caggcagggg ccggtaacga attcgacagc ttgttgttgg ccggggttta
    40021 gacgcatagg aaaatcgctc aatgaaagtc gggaggggaa aataacagcg tggtagtata
    40081 gccagcctca tccttaccac tcaaggcacg atcatggcaa aaacagcagc agcactgcat
    40141 atccttgtaa aagaagagaa actggctctg gatcttctcg agcagatcaa gaatggtgcc
    40201 gatttcggca agctggcgaa gaaacactcc atttgcccgt caggcaaacg cggcggtgat
    40261 ttaggtgaat tccgccaggg tcagatggtt ccggcgttcg ataaagtggt gttttcttgc
    40321 ccggtgctgg aaccaaccgg accgctgcat acccagttcg gttaccacat cattaaggtg
    40381 ctataccgta attaacagca aggccttctc cgggagaagg ccttgaactc tgaatcgcta
    40441 ttaacccgca acagcaatgc gtttcatatc tgtcatatag ccgcgcagtt tcttacctac
    40501 ctgctcaatc gcatggctgc gaatcgcttc atttacatca cgcagctgcg cgttatctac
    40561 cgcaccttcc ggaatagctt tacccaggtc gcccggttgc agctctgcca taaacggttt
    40621 cagcagtggc acacaagcgt aagagaacag atagttaccg tactcggcag tatcggagat
    40681 aaccacgttc atttcgtaca gacgcttacg ggcgatggtg ttggcaatca gcggcagttc
    40741 gtgcagtgat tcatagtaag cagattcttc gatgatgcca gaatcaacca tggtttcaaa
    40801 cgccagttca acgcctgctt ttaccatcgc gatcatcagt acgcctttat cgaagtactc
    40861 ctgttcgccg attttgcctt catactgcgg cgcggtttcg aatgcggttt tgccagtctc
    40921 ttcacgccag gtcagcagtt tcttatcgtc gttggcccag tcagccatca tgccggagga
    40981 gaattcgccg gagatgatgt cgtccatatg tttctggaac agcggcgcca tgatctcttt
    41041 cagttgctca gagagcgcgt aagcacgcag tttcgccggg ttagaaagac ggtccatcat
    41101 cagggtgatg ccgccctgtt tcagcgcttc ggtgatggtt tcccaaccga actgaatcag
    41161 tttttctgcg tatgccggat cggtgccttc ttccaccagc ttgtcgaagc acagcagaga
    41221 gccagcctgc aacataccgc acaggatggt ttgctcgccc atcaggtcag atttcacttc
    41281 cgcaacgaaa gaggattcca gaacgcccgc acggtgaccg ccggttgcag ccgcccaggc
    41341 tttagcgatc gccatgcctt cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt
    41401 cggtacgccg aatccacgtt tgtactcttc acgtacttcg gtgccagggc atttcggcgc
    41461 aaccattacg acggtgatgt ctttacggat ctgctcaccc acttctacga tattgaaacc
    41521 atgagagtag cccagcgccg cgccgtcttt catcagcggc tgaacggaac gcactacgtc
    41581 agagtgctgc ttgtctggcg tcaggttaac caccagatcc gcctgcggga tcagttcttc
    41641 gtaagtgcca actttgaagc cgttttcggt tgctttacgc caggatgcgc gcttctcggc
    41701 aatcgcttct ttacgcagag cgtaggagat atcgagacca gaatcacgca tgttcaggcc
    41761 ctggttcaga ccctgtgcac cacagccgac gatgactact tttttaccct gaagatagct
    41821 cgcgccatcg gcgaattcat cgcgccccat aaagcgacat ttgcccagct gtgccagctg
    41881 ctggcgcaga ttcagtgtat tgaagtagtt agccatggtg attcctcgtg atgttgtgct
    41941 tcttattgtt cggttcgcgt ttgcgagatt gaattcacta tatgacagga aatttattgc
    42001 ggaaattgat atattcacaa cgtcacattg caatttctgc aacgtcaaca ttaagggctg
    42061 cgccattgga tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc cgccattttg
    42121 gccgcagcgc gcgggcaatg cacgttagcc cgtccacgct ctctcggcag attcagcgcc
    42181 tggaagacga tctcggtcag ccgctgtttg tgcgcgataa ccgcacggta acgctaaccg
    42241 aagcaggcga agaactgcgc gttttcgccc agcaaacgct gttgcagtat caacagctgc
    42301 gccacaccat cgatcagcaa ggaccgtcgc tctctggcga attacatatc ttctgctcgg
    42361 tgaccgctgc ctacagccat ctgccgccga ttctcgatcg cttccgcgcg gaacacccgt
    42421 cggtggagat taaactcacg actggtgatg cggcagatgc gatggaaaag gtcgtcactg
    42481 gcgaagcgga tctggcgatt gccgggaaac cggagactct gcccggcgca gtggcgtttt
    42541 cgatgctgga gaatctggca gtagtgctga ttgcccccgc gctaccctgc ccggtgcgta
    42601 accaagtttc ggcagataag ccggactggt ctacagtgcc gtttatcatg gccgatcagg
    42661 gaccagtacg ccggcgcatt gagttgtggt ttcgacgcaa caaaatcagc aacccgatga
    42721 tttacgccac ggtcggcggg catgaagcga tggtgtcgat ggtggcgctc ggctgtggcg
    42781 tagccttgtt gccggaagtg gtgctggaaa acagcccgga accggtgcgt aaccgcgtga
    42841 tgattttaga gcgcagtgat gagaaaacgc cgtttgaact gggcgtgtgt gtgcagaaaa
    42901 agcggctaca tgagccgctg attgaggcat tctggaagat tttgccgaac cattaacccg
    42961 ccaaaaagaa cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtaa cccaactcat
    43021 tcaaccgggt ttcgaaatcc ggttcatggt cgccaagttc gaacgccgcc agtacgcgcc
    43081 cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc gtacccagcg
    43141 tgttgaggaa gcgcaacagc gcgcccggcg attccgggaa ttcgaagctg tagaggcgtt
    43201 cctgcaacgg atgcgacgga cgcccgccga ccatatagcg cacatgcagc ttcgccattt
    43261 cgtcgtcgga gagatccact acgctgtagc cgccatcgtt gagcatctgc aaaatttctt
    43321 tgcgctcttc gaggccacgg ctcaggcgca caccgacaaa gatgcaggcg tttttggcat
    43381 cggcaaaacg gtagttgaac tcggtgaccg agcgcccgcc aagcagctgg cagaacttca
    43441 ggaagctgcc cttctcttcc gggatggtta ccgccagcaa cgcttcacgc tgttcgccca
    43501 gttcgcaacg ttccgagacg tagcgcaggc cgtgaaagtt cacgttagca ccagaaagaa
    43561 tatgcgccag ccgctcaccg cgaatgttgt gctgggcgat atattttttc attcccgcca
    43621 gtgccagcgc gccagagggt tccgctaccg cgcgcacatc ttcgaacagg tctttcatcg
    43681 ccgcacatat ggcatcgcta tcgacggtga tgatgtcgtc gagatactcc tggcataaac
    43741 ggaaggtttc gtcgccgatg cgtttcaccg caacaccttc ggcaaacagc cccacgcgtg
    43801 gcagatcaac cggatgacct gcatccagcg ccgctttcag gcaggcggag tcttccgctt
    43861 ccacggcaat cactttgatt tgcggcatca gttgtttgat cagtaccgcc acgcccgccg
    43921 ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc tgctggagca
    43981 gttccagcgc cagcgtgcct tgcccggcga tcaccatcgg atggtcgaac ggcggcaccc
    44041 aggtgaagcc ctgctgctgt gacagttcga tcgctttcgc ttttgcttca tcaaagttcg
    44101 cgccgtgaag aagcacttcg ccgccgaagc cgcgcaccgc gtcgactttg atatcggcgg
    44161 tggcggttgg catgacgatc agtgccttca cgcctaaccg tgcggaagaa aacgcgacgc
    44221 cctgcgcgtg gttacccgca gaagcggtga tcacgccgtg cgctttctgt tcttccgtca
    44281 ggcccgccat catggcgtat gcgccgcgca gcttaaagct atgaactggc tgacggtctt
    44341 cgcgtttcac cagaatcacg ttatcgagac gcgacgagag tttttccatt ttttgtagcg
    44401 gcgtgacctg cgccgcttcg tagaccggcg cgcgcagcac cgctcttaaa tattcggcgc
    44461 cttccggggc gccggagaga ggttgtgagt ctgccatcat taacccccca gtttcgattt
    44521 atcacgcacc gcgcctttgt cggcgctggt tgccaggctg gcgtaggcac gcagcgcaaa
    44581 ggaaacctga cgttcacgat ttttcggtgt ccaggctttg ttgccacgag cttcctgcgc
    44641 ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac ggttcggaat
    44701 gtcgatagcg atcaagtcac catcttcaat caggccaatg ctgccgccac ttgccgcttc
    44761 cggtgagacg tggccgatgg aaagaccaga ggtgccgcca gagaaacgac cgtcggtgat
    44821 cagcgcacag gctttaccga gacccattga tttcaggaaa ctggttgggt agagcatttc
    44881 ctgcatcccc ggaccgcctt tcgggccttc atagcgaatt actaccacat ctccggcgac
    44941 aactttaccg ccgagaatcg cttctaccgc gtcgtcctgg ctttcgtaga ctttcgccgg
    45001 gccagtgaat ttgaggatgc tgtcatcgac gcctgcggtt ttaacgatgc agccgttttc
    45061 tgcgaaatta ccgtagagta ccgccaggcc gccgtctttg ctgtaagcgt gttccagcga
    45121 gcggatacag ccattggagc gatcgtcgtc cagcgtatcc cagcggcaat cctgcgagaa
    45181 tgcctgtgta gtacgaatgc ccgccgggcc agcgcggaac atatttttta ccgcatcatc
    45241 ctgggtcaca atgatgtcgt attgttccag cgtttgcggc aacgtcaggc caagtacatt
    45301 tttcacatca cggttcagta accccgcacg atccagctcg ccgagaatac cgataacacc
    45361 accagcacgg tgaacatctt ccatatggta tttttgggtg ctcggcgcga ctttacacag
    45421 ctgcggtact ttgcgggaga gcttatcgat atcactcatg gtgaagtcga tttccgcttc
    45481 ctgcgccgcc gccagcaggt gaagaacggt gttggtagag ccgcccatcg cgatatccag
    45541 cgtcatggcg ttttcaaacg ccgccttact ggcgatgtta cgcggcagtg cgctttcatc
    45601 atcctgttcg tagtaacgtt tggtcaattc aacaatgcgt ttaccagcat tgaggaacag
    45661 ttgcttgcga tcagcgtggg ttgccagcag cgagccgttg cccggctgcg acaggcccag
    45721 cgcttcggtc aggcagttca ttgagttagc agtaaacatc ccggagcagg aaccgcaggt
    45781 tgggcaggcg gatcgttcaa cctgatcgct ctgggagtca gaaactttcg ggtctgcgcc
    45841 ctggatcatc gcatcaacca gatcgagctt aatgatctga tcggacagtt tggttttccc
    45901 ggcctccatc gggccgccgg aaacaaagat caccggaata ttcaggcgca gggaagccat
    45961 cagcatcccc ggggtgattt tgtcgcagtt agagatgcag accatggcgt cggcgcagtg
    46021 ggcgttgacc atatattcaa cggaatcagc gatcagttcg cgagatggca gtgaataaag
    46081 catgcccccg tggcccatag cgatcccatc atccaccgca atggtgttga actctttggc
    46141 aacgccgccc gccgcttcaa tttgttcggc gaccagttta ccgagatcgc gcagatgaac
    46201 gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct taccgaaatc
    46261 ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat tacgaccatg
    46321 agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt ctgtctcgta
    46381 aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga tctaaccagc
    46441 cccatttatc ttcggtctcg ccagtgaaga ggccgaagaa ggcttgctga atgcgtttgg
    46501 taaccgggcc gcaacggcct tcaccaacct gaataccatc tacactgcgc actggcgtga
    46561 tttctgccgc cgtaccggac ataaagactt catccgccag gtacagggat tcgcgcgaca
    46621 gcacctgctc acgcacttca attcccagct ctttcgccag tttgatgatg gcatcacggg
    46681 taatacccgg cagcgcggag gaggtgaacg gtggagtaaa cagcacaccg tctttcactt
    46741 caaacaggtt ttcgcctgcg ccttcagaga tataaccatt cacatccaac gcgatacctt
    46801 cctgataacc gtggcggcgc gcttcgctac ccaccaacag ggaagagagg tagttaccac
    46861 cagctttcgc tgcggttggg atggtgtttg gtgcagcacg gttccaggag gaaaccatcg
    46921 catcgatccc ctgctccagc gcttctgcgc ccagatacgc cccccacggg aaagcggcga
    46981 taatcacatc ggttgagtat cccgctggcg ggtttacgcc catgccaaca tcaccgacga
    47041 aaatcagcgg acggatatag gcgctggtga gattgttttt gcggatcacg tcgcgacaag
    47101 cttccatcag ctcatcaatg ctctgcgaaa ccgggaaacg atagattttg gcggagtcat
    47161 gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgc gagtcgtagc
    47221 aacggatgcc ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc acatgcacct
    47281 tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct ttcttcgtgg
    47341 tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt tgtgctctgg
    47401 cagatagcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag taagtcgacc
    47461 gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct ggcggcagcc
    47521 atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac acgttctaag
    47581 gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat aatttctcca
    47641 acatttcaga attactggca ccaggcggca ccagcggcca gacgttctca agttcgtcga
    47701 ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt gccgcttcaa
    47761 cctggtcttt atgggtgatg tgttggccag ggatgccgaa ggcgctggct aacatgagga
    47821 aatcggggtt atcggtaagg gtggtttcgc tgtatcgttc ctggaaaaac agttgctgcc
    47881 attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac ggtaactgct
    47941 tgcgttttac ggtgcccagc tcttgcacat tcatcataaa agagccgtca ccggagatac
    48001 agacgacagt atcgttcggt cgcgcgactt gtgcgccaac cgccgccggt aaaccgaaac
    48061 ccatggtgcc taagccgctg gaggtgatga agttttccgg gcgagtgtgg acgatgtgct
    48121 gggcagccca catctggtgc tgccccacat ctgtggtcac gatgcaatcc gcaggtttac
    48181 gatccgacag ttgttttaac aacaacggcg cgtagatagc gtcaccagga tggtcgtaac
    48241 gccaggcatg ttcatcacgc agttgcgcgc agtgttgctg ccagtcatcg atatttaacg
    48301 gctgctgtaa tgctggtaac agagcattta aatcaccctg taatgccaca tgtgcctgac
    48361 gcagcttgtt catttctgcc ggatcgatat ccatatggat aacgctggcg tgtggtgcga
    48421 aggtgttcag tttgccggtc acccggtcat caaaacgtgc gcccacggct atcagtagat
    48481 cgcactcctg cactgcgaag ttcgccgctt tggtgccgtg catccccagc atgcccagat
    48541 agtacggata atctgcttct actgcgccca gccctttcaa cgtacaggtg gcaggcattt
    48601 ttgtggcagc gagaaattca cgcaaagccg aaactgcctg cgccatgccc acgccaccgc
    48661 caacgtacag catcggtttt tgcgcttttg ccagcatctg acgcgcttgc tcgacttcgg
    48721 catgtgggaa agtcacttcg ttttcaacag tggtgaacca cggttccaga tcgccgctgg
    48781 ctaactggat atcttttgga atatcgacca aaaccggacc aggacgacca gagctggcga
    48841 cgtcaaacgc ttcggccatg atacgcggta actcttccag tgattgcacc aggaaactgt
    48901 gcttggtgca ggctaacgac aaccccagaa tatccacctc ctgaaatgcg tcagtgccga
    48961 taaacggtgc ggatacctga ccggtgatag caacaacggg gatggaatct aacagcgcgt
    49021 ccgcaagccc ggttatcagg ttggttgcac ctgggccaga cgtggcgatg caaacgccag
    49081 ttttgccggt agcacgggca taaccgatag ccgccattgc tgcaccttgc tcgtgtcggc
    49141 acagcaagtg ttccacgcca ccgtcataca atgcatcgta aaccggcata attgcgccac
    49201 ccggataacc gaaaacggtg tttacaccct gtgcccgcaa cgcatgtacc acccactgtg
    49261 caccattcat agttagttcc ccgtcctgaa tcttgagaaa cagaattttg tgctgttatt
    49321 cattgtctgc tcctcagtta tgtttttaag gtcaaaaaaa acccccggac ctttcggtgc
    49381 gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag tgcagccccg
    49441 catggtggga taataatcac caccacgcta atcacgacca ggctaatcac tcgtagaagg
    49501 gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag ttaccataga
    49561 ttttgcgaat agcacaagat attttttggc cgatttttat gatgtcttta ataatctcaa
    49621 gagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaaattcaat ttcagtatga
    49681 atgttgttcc cttcctcgta gttcatcctt tgcccgctgc atttttgcga gcatcattcc
    49741 accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca ctaaaattca
    49801 cgaatttgcg ggctacagca gcgatatctt atgcacataa tggtgactca aggagggctt
    49861 atgtcactgt caattgttca tacccgcgca gccctgggag taaatgcgcc cccaatcact
    49921 gttgaggtac atatcagtaa aggtctacca ggcttaacga tggtgggctt accagaaaca
    49981 acggtaaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat
    50041 ccggcgaaaa aaatcaccat caaccttgct ccagccgatc tgccaaaaga agggggacga
    50101 tatgatttgc ctatcgctat tgcgttgctg gcggcctcag aacagcttac agccaataag
    50161 ttagatgaat atgaattagt cggagaactg gcgcttacag gcgctctgcg tggcgttccc
    50221 ggcgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa
    50281 gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctgatagc cgatcatctg
    50341 caggctgtct gcgcgtttct ggaaggtaag cacgctctcg aacacccgaa accaactaat
    50401 gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga gcaaggaaag
    50461 cgaggactgg aaattaccgc cgctggcggg cacaaccttt tactgattgg accgccggga
    50521 acaggtaaaa caatgctcgc cagccgtatt aatggccttt tgccagattt aagcaatgaa
    50581 gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa
    50641 tggcggcaac gcccgttccg ctcacctcat cacagtgcgt cgttaactgc gatggtgggc
    50701 ggtggtgcaa ttccagggcc aggtgaaatt tcgctggcgc ataacggcgt gctttttctt
    50761 gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc gattgaatcc
    50821 gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg tttccagctt
    50881 gtcgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa ccgctgcaca
    50941 ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga ccgcttcgat
    51001 ctctcactgg agatcccatt accgcctccc ggcattttga gtaaaacggt agtgccggga
    51061 gaaaacagca ccaccgttaa acaacgtgta atggccgcca gagagcgcca atttaagcgg
    51121 cagaataaac tgaacgcctg gctggataat ccggaaatac gccaattctg caagcttgag
    51181 agcgaagatg cgcagtggct ggaagaaacg ctgatccatc tggggttatc gattcgtgcc
    51241 tggcagcggt tattgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc
    51301 acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat
    51361 ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat cgtcagaatc
    51421 ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt
    51481 tggacgcttg atacgcgtca tatacttgga ccacacgcgt tctgcttctg tcactggttc
    51541 acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt
    51601 gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg cctctttgat
    51661 ggtgaagtca ccatgacgag agaatccacg tggataatgt ttattgtcga aatatcgatt
    51721 agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg agctagttat
    51781 ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa tcataacgac
    51841 aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc tggaagttag
    51901 ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccaat cagccgtgag
    51961 ctttcgaatc agacaactgg aaaatcaact gggtgtgaat cttttcaccc gccacagaaa
    52021 caatatccgt ttaaccgctg ccggagaaaa gcttctgcct tatgcagaaa cgctcatgag
    52081 cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataatg agttttctat
    52141 cggtgccagc gcctcgctgt gggagtgtat gcttaatcag tggctgggac gcttgtatca
    52201 aaatcaggat gtccatacag gcttacagtt cgaagcgcga attgcccaac ggcagtctct
    52261 ggtaaaacag ttgcatgaac gccagcttga tcttcttatt acaactgaag cccccaaaat
    52321 ggacgaattt tgtagtcagt tgctggggta tttcacttta gcgctttata ccagtgcccc
    52381 ttctaaacta aagggagatc ttaactatct gcgactagag tgggggccag actttcaaca
    52441 gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca gttctgctga
    52501 actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg tcagttgggc
    52561 acgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac ggccgcttta
    52621 tgccatatgg ctacaaaata gcgataaaaa tgcgttgatt cgcgatctat tgaaaattaa
    52681 cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag aaggatttac
    52741 ttcggagagg gttatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
    52801 caggggcgga gagactcgaa ctcccaacac ccggttttgg agaccggtgc tctaccaatt
    52861 gaactacgcc cctaattagg gtggcggaac ggacgggact cgaacccgcg accccctgcg
    52921 tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc
    52981 ggttttatga ccggcttact gc
//
LOCUS       DAOFSV010000015        52230 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.015, whole genome shotgun sequence.
ACCESSION   DAOFSV010000015 DAOFSV010000000
VERSION     DAOFSV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.015"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(157..1047)
                     /gene="rarD"
                     /locus_tag="QBH27_000998"
     CDS             complement(157..1047)
                     /gene="rarD"
                     /locus_tag="QBH27_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDQ7013338.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRISK"
     gene            complement(1099..1566)
                     /locus_tag="QBH27_000999"
     CDS             complement(1099..1566)
                     /locus_tag="QBH27_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDQ7013339.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1731..2600
                     /gene="pldA"
                     /locus_tag="QBH27_001000"
     CDS             1731..2600
                     /gene="pldA"
                     /locus_tag="QBH27_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDQ7013340.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2725..4554
                     /gene="recQ"
                     /locus_tag="QBH27_001001"
     CDS             2725..4554
                     /gene="recQ"
                     /locus_tag="QBH27_001001"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDQ7013341.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4619..5239
                     /gene="rhtC"
                     /locus_tag="QBH27_001002"
     CDS             4619..5239
                     /gene="rhtC"
                     /locus_tag="QBH27_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDQ7013342.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRCGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5277..6137)
                     /locus_tag="QBH27_001003"
     CDS             complement(5277..6137)
                     /locus_tag="QBH27_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDQ7013343.1"
                     /translation="MTEATTPHDAIFKAFLSRPETARDFIEIHLPPSLVKLCNLDTLH
                     LESSSFVEENLRQYYNDVLYSVETTKGCGYIYVLIEHQSSPDENMAFRMLRYAIATMQ
                     RHLEAGHDYLPLVIPILFYQGKRSPYPWSTNWLDGFPDPDIARDLYFHAFPLVDITLI
                     PDDEIMQHRSMAAFTLVQKHIRQRDMTTLLDKLSRLMILGQMSGQQIRMLINYMALVG
                     EAQDVRTLVHGLAQRVPQQGEELMTLAEELRRDALLNVAKAMLQRGFDLQLIMEMTGL
                     SQEDLYQLQS"
     gene            complement(6296..6916)
                     /gene="rhtB"
                     /locus_tag="QBH27_001004"
     CDS             complement(6296..6916)
                     /gene="rhtB"
                     /locus_tag="QBH27_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDQ7013344.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7027..8049
                     /gene="pldB"
                     /locus_tag="QBH27_001005"
     CDS             7027..8049
                     /gene="pldB"
                     /locus_tag="QBH27_001005"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDQ7013345.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHSGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8057..8857
                     /gene="yigL"
                     /locus_tag="QBH27_001006"
     CDS             8057..8857
                     /gene="yigL"
                     /locus_tag="QBH27_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDQ7013346.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            8933..9832
                     /gene="bioP"
                     /locus_tag="QBH27_001007"
     CDS             8933..9832
                     /gene="bioP"
                     /locus_tag="QBH27_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDQ7013347.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9720..10673)
                     /gene="metR"
                     /locus_tag="QBH27_001008"
     CDS             complement(9720..10673)
                     /gene="metR"
                     /locus_tag="QBH27_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDQ7013348.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10791..13052
                     /gene="metE"
                     /locus_tag="QBH27_001009"
     CDS             10791..13052
                     /gene="metE"
                     /locus_tag="QBH27_001009"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDQ7013349.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NIDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            13391..13948
                     /locus_tag="QBH27_001010"
     CDS             13391..13948
                     /locus_tag="QBH27_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDQ7013350.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDTIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            13990..15498
                     /locus_tag="QBH27_001011"
     CDS             13990..15498
                     /locus_tag="QBH27_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDQ7013351.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGAPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            15549..17546
                     /gene="tkt"
                     /locus_tag="QBH27_001012"
     CDS             15549..17546
                     /gene="tkt"
                     /locus_tag="QBH27_001012"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ7013352.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(17576..18421)
                     /locus_tag="QBH27_001013"
     CDS             complement(17576..18421)
                     /locus_tag="QBH27_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDQ7013353.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(18621..19526)
                     /locus_tag="QBH27_001014"
     CDS             complement(18621..19526)
                     /locus_tag="QBH27_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7013354.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNQQLMTRFVFN"
     gene            complement(19604..20827)
                     /locus_tag="QBH27_001015"
     CDS             complement(19604..20827)
                     /locus_tag="QBH27_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDQ7013355.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(20853..21242)
                     /locus_tag="QBH27_001016"
     CDS             complement(20853..21242)
                     /locus_tag="QBH27_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HDQ7013356.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(21259..22215)
                     /gene="arcC"
                     /locus_tag="QBH27_001017"
     CDS             complement(21259..22215)
                     /gene="arcC"
                     /locus_tag="QBH27_001017"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDQ7013357.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(22208..23632)
                     /locus_tag="QBH27_001018"
     CDS             complement(22208..23632)
                     /locus_tag="QBH27_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDQ7013358.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMARYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(23629..25188)
                     /gene="fdrA"
                     /locus_tag="QBH27_001019"
     CDS             complement(23629..25188)
                     /gene="fdrA"
                     /locus_tag="QBH27_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDQ7013359.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSKEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(25283..26143)
                     /locus_tag="QBH27_001020"
     CDS             complement(25283..26143)
                     /locus_tag="QBH27_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDQ7013360.1"
                     /translation="MVQVTLCASSLGYLFPQDETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(26149..26817)
                     /locus_tag="QBH27_001021"
     CDS             complement(26149..26817)
                     /locus_tag="QBH27_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDQ7013361.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(27074..27883)
                     /locus_tag="QBH27_001022"
     CDS             complement(27074..27883)
                     /locus_tag="QBH27_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDQ7013362.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLGGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAEHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            28145..28909
                     /gene="udp"
                     /locus_tag="QBH27_001023"
     CDS             28145..28909
                     /gene="udp"
                     /locus_tag="QBH27_001023"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDQ7013363.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(28944..30434)
                     /locus_tag="QBH27_001024"
     CDS             complement(28944..30434)
                     /locus_tag="QBH27_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HDQ7013364.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(30447..30953)
                     /locus_tag="QBH27_001025"
     CDS             complement(30447..30953)
                     /locus_tag="QBH27_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HDQ7013365.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(30970..31944)
                     /locus_tag="QBH27_001026"
     CDS             complement(30970..31944)
                     /locus_tag="QBH27_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HDQ7013366.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(31969..32598)
                     /locus_tag="QBH27_001027"
     CDS             complement(31969..32598)
                     /locus_tag="QBH27_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDQ7013367.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFTAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(32588..33592)
                     /locus_tag="QBH27_001028"
     CDS             complement(32588..33592)
                     /locus_tag="QBH27_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDQ7013368.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPSLQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDELHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(33616..34383)
                     /locus_tag="QBH27_001029"
     CDS             complement(33616..34383)
                     /locus_tag="QBH27_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDQ7013369.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            34600..36027
                     /gene="rmuC"
                     /locus_tag="QBH27_001030"
     CDS             34600..36027
                     /gene="rmuC"
                     /locus_tag="QBH27_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDQ7013370.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYQTQVSIETDTRSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQNLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            36122..36877
                     /gene="ubiE"
                     /locus_tag="QBH27_001031"
     CDS             36122..36877
                     /gene="ubiE"
                     /locus_tag="QBH27_001031"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDQ7013371.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            36891..37496
                     /gene="ubiJ"
                     /locus_tag="QBH27_001032"
     CDS             36891..37496
                     /gene="ubiJ"
                     /locus_tag="QBH27_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDQ7013372.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            37493..39133
                     /gene="ubiB"
                     /locus_tag="QBH27_001033"
     CDS             37493..39133
                     /gene="ubiB"
                     /locus_tag="QBH27_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDQ7013373.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            39212..39481
                     /gene="tatA"
                     /locus_tag="QBH27_001034"
     CDS             39212..39481
                     /gene="tatA"
                     /locus_tag="QBH27_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDQ7013374.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            39485..40000
                     /gene="tatB"
                     /locus_tag="QBH27_001035"
     CDS             39485..40000
                     /gene="tatB"
                     /locus_tag="QBH27_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDQ7013375.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            40003..40779
                     /gene="tatC"
                     /locus_tag="QBH27_001036"
     CDS             40003..40779
                     /gene="tatC"
                     /locus_tag="QBH27_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDQ7013376.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            40821..41603
                     /gene="tatD"
                     /locus_tag="QBH27_001037"
     CDS             40821..41603
                     /gene="tatD"
                     /locus_tag="QBH27_001037"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDQ7013377.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNAPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(41600..42088)
                     /gene="rfaH"
                     /locus_tag="QBH27_001038"
     CDS             complement(41600..42088)
                     /gene="rfaH"
                     /locus_tag="QBH27_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDQ7013378.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            42255..43748
                     /gene="ubiD"
                     /locus_tag="QBH27_001039"
     CDS             42255..43748
                     /gene="ubiD"
                     /locus_tag="QBH27_001039"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDQ7013379.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            43794..44495
                     /gene="fre"
                     /locus_tag="QBH27_001040"
     CDS             43794..44495
                     /gene="fre"
                     /locus_tag="QBH27_001040"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDQ7013380.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(44676..45839)
                     /gene="fadA"
                     /locus_tag="QBH27_001041"
     CDS             complement(44676..45839)
                     /gene="fadA"
                     /locus_tag="QBH27_001041"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDQ7013381.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(45849..48038)
                     /gene="fadB"
                     /locus_tag="QBH27_001042"
     CDS             complement(45849..48038)
                     /gene="fadB"
                     /locus_tag="QBH27_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDQ7013382.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            48228..49559
                     /gene="pepQ"
                     /locus_tag="QBH27_001043"
     CDS             48228..49559
                     /gene="pepQ"
                     /locus_tag="QBH27_001043"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDQ7013383.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            49559..50173
                     /locus_tag="QBH27_001044"
     CDS             49559..50173
                     /locus_tag="QBH27_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDQ7013384.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            50212..51663
                     /gene="trkH"
                     /locus_tag="QBH27_001045"
     CDS             50212..51663
                     /gene="trkH"
                     /locus_tag="QBH27_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDQ7013385.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            51675..52220
                     /gene="hemG"
                     /locus_tag="QBH27_001046"
     CDS             51675..52220
                     /gene="hemG"
                     /locus_tag="QBH27_001046"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDQ7013386.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        12420 a        13122 c        13907 g        12781 t
ORIGIN      
        1 gaaaatatat agcggcgtct ggaaattctg cctttagatg ttggaataaa tatacgcaac
       61 attaatttta catccttgtg taagaatttc gttagcatgg caatattccc gctcttatgc
      121 aagagcaggc atattttttc agaatatatt tattttttat ttggaaattc tacgctgcgt
      181 ataaatcgca tccatcacaa aaattgccag cgccacccaa ataaaggcga aagtcaccat
      241 cttatcggca cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg
      301 accaatgtac tggaaaaagc ctaacgttga gagacgcagg cgtgtcgcag ctgcagtaaa
      361 acacaacagt ggcacggtgg tgacaatacc ggcggcgatc agcagtaaat tcagcgacat
      421 cgggttttgc cccatatgac tggttgagct gtcggcaata gcaaacaggt aaatcgccgc
      481 cacgggcagc aaccacatag tttcgattaa catgccggtt tgcgcttcaa cagcaatctt
      541 cttgcgcacc aggccgtaga aagcaaaact aaacgccagc cccagcgcga tgatcggcag
      601 tgaaccaaaa gtccacaact gaaccaacac gccacaaatc gccagaatca ccgccagcca
      661 ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagtgg
      721 gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa
      781 cagtagccag ttgccaccaa tcagcacggc agagactgcc agcataaaaa ttttctgtgg
      841 cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat
      901 aaagaaaaac gaccagatca cgcgatgcgt caggatctca tcggcgggca cgtagtaaat
      961 cagtttgaag tacgctggcg caatacccca aataaaataa gcggcaagag cgagtaatac
     1021 gccctgccgc gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt
     1081 ttacctgctt ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag
     1141 ctgttcttca ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag
     1201 gctactggta gcagtaaaac gctctcctct gcctgggcgc agataatcga cgcgaagatc
     1261 aatggtcccc atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg
     1321 ggttaaggta cttcccacgc acaccagacc tgcggcgaca tccagcgccg acgcaatgac
     1381 cccgccgtgt aaaatgcttt gcgcccagtt gcccaccatc atcggctgat ttttaaaggc
     1441 cagctgtgcg aactcttttt cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg
     1501 catgtgataa acaaacatct cacccactaa tttgagggct tgttcagcgg tcagtacggc
     1561 agacataaca tccttacact tcattagtta atgaaatgtt gattttatgc ttatttattg
     1621 ttggtttcta ctttaggaag ggataactaa cggctatgga gttaagtatg taaaatagcc
     1681 cgcagaaaaa tattcacctt atcaataatt cgttacggag aacacgagcg atgcggactc
     1741 tgcagggctg gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa
     1801 cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc
     1861 aggagcatga caatccgttc accctttatc cttatgacac caactacatc atctacaccc
     1921 aaaccagcga tctgaataaa gaagcgattg ccagttacga ctgggcggaa aacgcgcgta
     1981 aggatgaagt aaagttccag ttgagcctgg cgtttccgct gtggcgtggg attttggggc
     2041 caaactctgt actgggcgcc tcctacacac agaaatcctg gtggcagctt tctaatagcg
     2101 atgagtcatc accgtttcgt gaaactaact acgaaccgca attgttcctc ggttttgcca
     2161 cggattacaa ctttgctggc tggacgctgc gcgatgtgga aatgggctac aaccacgact
     2221 caaacgggcg ttcagacccg acttcccgca gctggaaccg cctttatacc cgcctgatgg
     2281 cagaaaacgg taactggttg gtagaggtga agccgtggta tgtggtgggg aatacggacg
     2341 ataacccgga catcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg
     2401 gtgatgcggt actgagcgcg aaagggcagt acaactggaa caccggttac ggcggcgcgg
     2461 agttaggctt aagctacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg
     2521 gttacggcga atcgctcatc gactataact tcaaccagac ccgcgttggt gtgggagtta
     2581 tgctaaacga cctgttttga tgaatggttg gcaaatctgg aatccagcat ccaggattac
     2641 cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt
     2701 ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct ggagtccgga
     2761 gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg ccaggaagag
     2821 attatcgaca ccgtactttc cggtcgcgat tgcctggtcg tcatgcccac tggtggcgga
     2881 aaatcccttt gctatcaaat ccctgcctta ttgctaaacg gccttaccgt ggttgtctca
     2941 ccgctgattt cgttgatgaa agatcaggta gatcaactgc aagccaacgg cgtggcggcg
     3001 gcgtgcctta actcgacgca aacccgcgag cagcaacttg aagtgatgac aggctgtcgc
     3061 accgggcaaa tcagcctgct ttatatcgcg ccggaacgcc tgatgctgga taactttctt
     3121 gagcatctgg cgcactggaa tccggtgtta ttagccgttg atgaggcgca ctgtatctcc
     3181 caatggggtc acgatttccg cccggaatac gctgccctcg gtcagctgcg tcagcgtttt
     3241 ccgacgctgc cgtttatggc gctgactgcc acagccgatg acaccacgcg ccaggatatc
     3301 gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga ccgtccgaat
     3361 attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg ttatgtacag
     3421 gaacaacgcg gtaagtcggg gattatttac tgtaatagtc gtgcgaaagt agaagacacc
     3481 gctgcgcgcc tgcaaagcaa gggaattagc gcggcggcct atcatgccgg gctggaaaat
     3541 aatgttcgcg ctgatgtgca ggaaaaattc cagcgcgatg acctgcaaat tgtggtggcg
     3601 acggtggcgt tcggcatggg catcaacaag cctaacgttc gcttcgtggt gcattttgat
     3661 attccgcgca atatcgaatc ctattatcag gaaactggac gcgccgggcg tgatggcctg
     3721 cccgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg ccgttgtctg
     3781 gaagagaagc cgcaggggca gttgcaggat atcgaacgtc ataaactcaa tgcgatgggc
     3841 gcgtttgctg aagcgcaaac ttgccgtcgt ttggtattgc tgaactattt tggcgaaggg
     3901 cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccaaaaca gtacgatggt
     3961 tcaaccgatg cgcagattgc cctttccacc attggtcgtg tgaatcagcg gtttgggatg
     4021 ggttatgtgg tggaagtgat tcgcggtgct aataatcagc gtatccgcga ctatggtcat
     4081 gacaaactga aagtctatgg catgggccgt gataaaagcc atgaacattg ggtgagcgtg
     4141 atccgccagc tgattcacct cggcctggtg acgcaaaata ttgcccagca ttctgcccta
     4201 caactgacag aggctgcgcg tccggtgctg cgcggcgaat cctctttgca acttgccgtg
     4261 ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg caactatgat
     4321 cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga aagcaatgtc
     4381 ccgccgtacg tggtgtttaa cgacgctacc ttgattgaga tggctgaaca gatgccgatc
     4441 accgccagcg aaatgctcag cgttaacggc gttggtatgc gcaagctgga acgctttggc
     4501 aaaccgttta tggcgctgat tcgtgcgcat gtcgatggtg acgacgaaga gtagtcagca
     4561 gcataaaaaa gtgccaggat gaagactccg taaacgtttc cccccgcgag tctaatgtat
     4621 gttgatgtta ttcctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     4681 cgatttcttt tttgtatctc agaccgctgt tagtcgttcc cgtaaagaag cgatgatggg
     4741 cgtgctgggc attacctgcg gcgtaatggt ttgggctggg attgcgttgc ttggcctaca
     4801 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggtgggcg gtggcctgta
     4861 tctctgctgg atgggttacc agatgctacg tggtgcactg aaaaaagagg ttgtttctgc
     4921 ccctgcgcca caagtcgaac tggcgaaaag cgggcgcagt tttcttaagg gcttactgac
     4981 caatctcgcg aatcctaaag cgattatcta ctttggttcg gtgttctcgc tgtttgtcgg
     5041 tgataatgtc ggcaccaccg aacgctgggg cattttcgcg ttgattattg tcgaaacact
     5101 ggcgtggttt accgtcgttg ccagcctgtt tgctctgccg caaatgcgct gtggttatca
     5161 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5221 tttgattatt tcgcgttaag cgcttccccc ctgatacgct atcaggggga ataacatcag
     5281 gattgcaatt gataaaggtc ttcctgagaa agcccggtca tttccataat caactgcaaa
     5341 tcaaagccac gctgtagcat ggcttttgcg acattgagaa gggcatcgcg ccgaagttct
     5401 tccgctaaag tcatcagttc ctctccttgt tgaggcactc gttgtgccag accatgtact
     5461 aatgttcgta catcctgtgc ttcacctacc agcgccatgt agtttatcaa catgcgtatt
     5521 tgctgtccgc tcatctgccc cagaatcatt aagcgggata acttatctaa cagcgttgtc
     5581 atatcccgtt gacggatatg cttctgtacc agcgtaaagg cggccatgct gcggtgttgc
     5641 atgatttcgt catcaggaat tagcgtaata tcaaccagcg gaaaagcatg aaaatagaga
     5701 tcgcgtgcaa tgtccggatc cggaaaacca tcgagccagt ttgttgacca tggatatggg
     5761 ctacgctttc cctggtaaaa aagaatgggg atcaccagag gcagataatc atgtcccgct
     5821 tcaagatgcc gctgcatggt ggcaatagca tagcgaagca tacggaacgc catattttca
     5881 tcaggtgaac tttgatgctc aatcaacaca tagatatatc cgcacccttt cgtcgtctct
     5941 actgaataaa gtacgtcgtt gtaatactgg cgtaggtttt cctctacaaa actgctggac
     6001 tcgagatgga gcgtatcaag attgcagagt ttaactaaag aaggcggcag atgaatttca
     6061 ataaagtcac gggccgtttc tggtcgggat aaaaaagcct taaagattgc gtcatgtggt
     6121 gtcgttgctt ccgtcatttt tcatcatccg tgggtgaaat atggcggcat tctggcacta
     6181 acaaagagaa gtaaatagta aataaattaa cagattacgc ttattacgag atgcctcgag
     6241 cgcggatgta acaatctctc gaggcacatt aatttacgtg agctttattc cagttttaag
     6301 catgcctcgc cgatgctaac agcgctccca ccagcataaa taacgagccg aaaatcttat
     6361 tcagcgcctt catctgcttt ggtcctttaa tccatagagc aatccgttga gcaagggtgg
     6421 cgtaaccgat catcacaata atatcgacca caatagtggt gacgccgagc acgatatact
     6481 gcatcagctg cggctgttgc ggcatgatga attgcggaaa tagcgccgcc agaaacacaa
     6541 tacttttggg attggtgaga ttgacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt
     6601 gagtagaggc cagcgattta agatcaattg cgccagcggc gcgccactgc tggatcccaa
     6661 gccaaatcaa gtaagctgcg cctgcccact tcaacacttc aaacgcaatc actgagcggg
     6721 aaaatagcgt ccccaacccc acgccaacca gcacaatatg aatcgccagt ccggtctgaa
     6781 gcccagcaat agacgccacc gcgccgcgat aaccgtggtt gagcgaggtg gtcatggtgt
     6841 tgattgcgcc agagcctggc gacaggctta aaatgatcga tgtcagcagg taggcaaacc
     6901 accattctaa ggtcatgatg aactcccggt gtgtctattt ttgtgccaca atacgctact
     6961 gtcgcagcgt tgtgtcaggc acgcttaaaa aaacgatttt acgtggttta agaggcagat
     7021 tacccgatgt ttcagcagca aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt
     7081 accatgggac cgctgactga tttctggcgt cagcgtgatg aagcagagtt tactggtgtg
     7141 gatgacattc cggttcgctt tgtccgtttt cgcgcacagc accatgaccg ggtggtagtt
     7201 atctgcccgg ggcgtattga gagctacgta aaatatgcgg aactggccta cgacctgttc
     7261 cattcggggt ttgatgtttt aatcatcgac catcgcgggc agggacgttc cggtcgcctg
     7321 ttagccgatc cacatctcgg gcatgttaat cgctttaatg attatgttga tgatctggcg
     7381 gcattctggc aacaggaggt tcagcccggt ccgtggcgta aacgctatat actggcacat
     7441 tcgatgggcg gtgcgatctc cacattattt ctgcaacgcc atccaggtgt atgtgacgcc
     7501 attgcgctaa ctgcgccaat gtttgggatc gtgattcgta tgccgtcatt tatggcacgg
     7561 cagatcctca attgggccga agcgcatcca cgtttccgtg atggctatgc aataggcacc
     7621 gggcgctggc gcgcgttgcc gtttgctatc aacgtactga cccacagcag acagcgatat
     7681 cgacgtaact tacgtttcta tgctgatgac ccaacgattc gcgtcggtgg gccgacctac
     7741 cattgggtac gcgaaagtat tctggctggc gaacaggtgt tagccggtgc gggtgatgac
     7801 gccacgccaa cgcttctctt acaagctgaa gaggaacgcg tggtggataa ccgcatgcac
     7861 gaccgttttt gtgaactacg caccgctgcg ggccatcctg tcgaaggagg acggccgttg
     7921 gtaattaaag gtgcgtacca tgagatcctt tttgaaaagg acgcaatgcg ctcagtcgcg
     7981 ctccacgcta tcgttgattt tttcaacagg cataactcac ccagcggaaa ccgctctaca
     8041 gaggtttaaa tttcttatgt accaggttgt tgcgtctgat ttagatggca cgttactttc
     8101 tcccgaccat acgttatccc cttacgccaa agaaactctg aagctgctca ccgcgcgcgg
     8161 catcaacttt gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa
     8221 tctggagatt aagtcttaca tgattacctc caatggcgcg cgcgttcacg atctggatgg
     8281 caacctgatt tttgctcata acctggatcg cgacattgcc agcgatctgt ttggcgtagt
     8341 caacgacaat ccggacatca ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg
     8401 ccatcgcccg gaagagatgc gctttttcaa agaagcggtg ttcaattatg cgctgtatga
     8461 gccgggattg ctggagccgg aaggcgtcag caaagtgttc ttcacctgcg attcccatga
     8521 aaaacttctg ccactggagc aagcgattaa cgctcgctgg ggcgatcgcg tcaatgtcag
     8581 tttctctacc ttaacctgcc tggaagtcat ggctggcggc gtttcaaaag gccatgccct
     8641 ggaagcggta gcgaagaaac tgggttacag cctgcaagat tgtattgcct ttggcgatgg
     8701 aatgaacgat gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg gcagcgcgca
     8761 tcaacggctg aaagatctgc atccagagct ggaagtgatt ggtactaacg ccgaagacgc
     8821 agtgccgcat tatctgcgta agctctattt atcgtaatcg ttctttattt ggtcagttgt
     8881 caacctgata cttcgctaca atggatactc gttaatcaaa gagttttcca ttgtggcgct
     8941 acttatcatc accacgattc tgtgggcctt ctcctttagc ttttatggcg agtaccttgc
     9001 ggggcacgtc gatagctatt ttgcggtgct ggtgcgcgtt ggtctggcgg cacttgtttt
     9061 tctgccgttt ctgcgtaccc gtggcaatag cctgaaaacg gtcggcctgt atatgctggt
     9121 gggcgcgatg cagctcggtg tgatgtatat gctgagtttc cgcgcctatc tctacctgac
     9181 ggtttctgaa ctgctgttgt tcaccgtact gacgccgctc tacatcacgc tgatttatga
     9241 catcatgagt aagcgccgtc tgcgctgggg ctatgctttt agcgccttgt tggcggtgat
     9301 tggtgccggg attattcgct atgatcaggt caccgaccat ttctggactg gcttgttgct
     9361 ggtgcaactc tccaatatca cttttgccat cggcatggtg ggttacaaac gtctgatgga
     9421 aactcgcccg atgccgcagc ataacgcctt tgcgtggttc tatcttggcg cgttcctggt
     9481 ggcagtgatt gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca
     9541 atggggcatt ctggtgtttc ttggcgtggt ggcttccggg attggctact ttatgtggaa
     9601 ctacggcgcg acgcaggtgg acgccggaac gctgggcatt atgaataata tgcacgttcc
     9661 ggcagggctg ctggtaaacc tggctatctg gcaccaacag ccgcactggc caacgtttat
     9721 tacaggcgcg ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg
     9781 ctcttcacaa acggcagatg atcgcaggcg tgattgcgcg ctgaacgaat aaacgcttct
     9841 gtaatcggct gacgctgctc gccatcgcgc acggcggcgt acagtcggct ccacaagcct
     9901 tcgcccaggg ttttggtgac caccagaccc tggcgttcaa aactctccac cacccaatga
     9961 ggtagtgcgg caatacccat ccgtgcggca accatctgaa tcaacaataa ggtgttatcg
    10021 acgcttttca gtgacgggct gacgcctgct ggctgaagaa aatgccgcca gacatccagt
    10081 cggctacgct gcaccggata aatcaacagc gtttcgctgg cgagatcttc cggtgtaata
    10141 cgcgtttttg ccgccagtgg atggtcaggt gctaacacca gacgtacttc atagtcgaac
    10201 atcggcgaat aatgcaggcc actgcgcggc agaatatcgg acgtcatcac cagatccagc
    10261 tcgccctgtt gcaacgcagg ctgcggatca aacgttacgc ccgatttaaa atccatctcc
    10321 acctgcggcc agttcttatg gaaattttct aatgcgggcg tcagccactg aatacagcta
    10381 tggcactcta tggcaatgcg caggcgcgtc tgctgcggtt cattgcaggc ttgcagtgcc
    10441 tggctaattt gcggcagcac ctgatttgcc agttgcaaca ggatttctcc ctgcggtgta
    10501 aagcgaagcg gctggctttt acgcacaaat agccggaagc caaggcgttg ttccagatcg
    10561 ctaaactggt gagacagggc ggattgcgtt tgatgcaacg tcgccgcagc ggctgcgagt
    10621 gagccgcagt tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc
    10681 cttcacttcg gcatgaataa tttgcgcttg aggaatatac agtaaccgcc aattatggat
    10741 gtgtaaacat ctggacggct aaatactaaa aattccataa ggggcatata atgacaattc
    10801 ttaatcacac cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag
    10861 agagttattg ggcggggaac tccacgcgtg aagaactgct ggcggtaggg cgtgaattgc
    10921 gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga cctgctgccg gtgggcgatt
    10981 ttgcctggta cgatcatgta ctgacaacca gtctgctgct gggtaatgtt ccgcaacgtc
    11041 atcagaacaa cgatggttcg gtagatatcg ataccctgtt ccgtattggt cgtggacgtg
    11101 caccgactgg cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac accaactatc
    11161 actacatggt gccggagttc gttaaaggcc aacagttcaa actgacctgg acgcagctgc
    11221 tggaggaagt ggacgaggcg ctggcgctgg gccacaaggt gaaacctgtg ctgctggggc
    11281 cggttaccta cctgtggctg ggtaaagtga aaggtgaaca gtttgatcgc ctgagcctgc
    11341 tgaacgacat tctgccggtt tatcagcaag tgctggcaga actagcgaaa cgcggcattg
    11401 agtgggtaca gatcgacgaa cccgcgctag tgctggaact gccgcaggcg tggctggatg
    11461 catacaaacc cgcttacgac gcgctccagg gccaggtgaa actgctgctg accacctatt
    11521 ttgaaggcgt aacgccaaac ctcgacacga ttactgcgct gcctgttcag ggtctgcatg
    11581 tcgatcttgt acatggtaaa gatgacgttg ctgaactgca caagcgcctg ccttctgact
    11641 ggctgctgtc tgcgggtctt atcaatggtc gtaacgtctg gcgcgccgat cttaccgaga
    11701 aatatgcgca aattaaggac attgtcggca aacgtgattt gtgggtggca tcttcctgct
    11761 cactgctgca cagccccatc gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa
    11821 gttggtttgc cttcgccctg caaaaatgtc atgaactggc attgctgcgc gatgcgctga
    11881 acagtggtga tacggcagct ctggcagagt ggagcgctcc gattcaggcg cgtcgtcact
    11941 ctactcgtgt acataatccg gcagtagaaa agcgtctggc ggcgatcacc gcccaggaca
    12001 gtcagcgtgc gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt tttaaactgc
    12061 ccgcgtggcc gaccaccacg attggttcct tcccgcaaac cacggagatt cgtaccctgc
    12121 gtctggattt taaaaagggt aatatcgacg ccaataacta ccgcacgggc attgcggaac
    12181 atatcaagca ggccattgtt gagcaggaac gtttgggact ggatgtgctg gtacatggcg
    12241 aggccgagcg taatgacatg gtggaatact ttggcgagca tctggatggc tttgtcttta
    12301 cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt gaagccaccg attgttattg
    12361 gtgacgttag ccgcccggca ccgattaccg tggagtgggc aaaatatgcg caatccctga
    12421 ctgataaacc ggtgaaaggg atgttgactg gcccggtgac tattctctgc tggtcgttcc
    12481 cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat tgcgctggca ctgcgtgatg
    12541 aagtggccga tctggaagcc gctggaattg gcatcattca gattgacgaa ccggcattgc
    12601 gcgaaggttt accactgcgt cgcagcgact gggatgccta tctccagtgg ggcgtggagg
    12661 ctttccgtat caacgccgcc gtggcgaaag atgacacaca aatccacact cacatgtgtt
    12721 actgcgagtt caacgacatt atggattcga ttgcggcgtt ggacgcagac gtcatcacca
    12781 tcgaaacctc gcgttccgac atggagttgc tggagtcgtt tgaagagttt gattatccaa
    12841 atgaaatcgg tcctggcgtc tatgacattc actcgccaaa cgtaccgagc gtggaatgga
    12901 ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc agagcgtctg tgggtcaacc
    12961 cggactgtgg cctgaaaacg cgcggctggc cagaaacccg cgcggcactg gcgaacatgg
    13021 tgcaggcggc gcagaatttg cgtcgggggt aaaatccaaa ccgggtggta ataccacccg
    13081 gtcttttctc attacagcga cgtcttccca gcatactgcg taaaccattc cagatatcag
    13141 ttcataccct gtttaagaaa atcttcttta ttcccttctc atgaaataaa tttcgtcatt
    13201 tccgccattt tttctatctg cctctccatc acatattaaa aattacttga tatctctatg
    13261 ttttttaatg aaaaacatat caacctatga tctttcttgt gactagcttc acatttcata
    13321 ctgatgcatt ggaatgaaat aaaatttcat gagattttgt gtttgagaaa attatttcat
    13381 gaggtgtgaa atggacgcaa agctgtacat cactgagatc atcaatgaat taaccttcgt
    13441 taaggactct atccgtgagg atgactgcac caaattgatc gacactattc agcaaagcca
    13501 gcgcgttttc tgctatggcc tgggacgcgc cggtttttct atgaaagcct tcaccatgcg
    13561 cctgatgcat atgggaaaag aggtttactt cctgacggaa accattacgc cgaactttgg
    13621 gcctggcgat ctgtttatcg tttcttctgc ttccggtgag acagcacaac tggtggcgct
    13681 ggcgaaaaaa gcacgtcagc tcggcggcgc tgtggcggtg ttaaccacga atcgacacgc
    13741 cactatcacc gagtttgtcg acgtcatcgt gcaaatcaat gccccttcca aaaatcaaaa
    13801 agattctgtt ttccgctcgg cacagccgat ggcgtcgttg tatgagcaag ctttgctggt
    13861 gattgccgat gcattagtga tgaagatggc tgctgaaagt ggtgcgccgg aaagtgaact
    13921 gtttaagcgc cacgccaatc tggaatagtc gtcgccgtta tttgctcgat accctacagg
    13981 agaataaaaa tgagcaaaaa aataacatcc ttgtttaact tcaagaagtt gcagaaattt
    14041 tcttctgctc tgatgttacc aatcgccatt cttccggcgg cgggtttgat tttagccatc
    14101 gggatttcat ttaacattga gctgctggcg aattgcggcg gcctgatttt cgccaacctt
    14161 cctttgctgt tttgtgtcgg tattgtgatt ggcctgacag gtgatggttc ttcatcactt
    14221 tcagcgatta tcagttacat gatcatgaat gccacgatgg gaacgcaact tggcattact
    14281 gcggagtccg ttgcagccag taacggtgat tatgcgatga tcctcggggt accgacgtta
    14341 caaacgggag tgtttggcgg tctgattgtc gccatctttg tttatctgct ctatacccgc
    14401 ttccacaata ttgaattgcc tcagtttctt ggtttcttcg gtggaaaacg ctttgtaccg
    14461 attattacgg ctgtggcggc aattattgtc ggctatttat tgccgtggct gtggataccg
    14521 gtacaaaaag gtctggcgtc gctttcaacg ctggtgacca gtggacataa cgcgaacttc
    14581 gcggccttta tttacgccgt tggcgaacgt tcgctgattc cttttggcct gcatcatatc
    14641 tggaacgtgc cgttttatta caactttggc gactacatga ccaaatccgg gcaactggtg
    14701 acgggcgata ttccggtgtt ctttgcccaa ttgcgcgacg gtgctccgtt gacggcgggg
    14761 ctgtttatga caggtcgttt cccgatcatg atgtttgcgt tacctgccgc cgcgttggcg
    14821 atgtatcagg aagcaaaacc ggaacgtaag gcgctgatta aaggcctttt actctccggt
    14881 gcgctgacca catttgttac cgggattacg gagccgctgg aatatgcatt tttgtttgca
    14941 gcgccggttc tctacatcat tcacatcttc ttatatgcca cttcatttgt gctgatgaac
    15001 atcctgaatg tgcacattgg tcatcctttt gctggcggtc ttattgattt cgtactgaat
    15061 ggcgttatgc caaaccgcac gccgtggtat ctggtgttcc tggcaggcgc gggctatgca
    15121 gcgatttact acaccgtatt tcgggtactg atccgcaagt tgaatctgaa aacaccgggg
    15181 cgtgaggatg aagagattgc cgttgcaacc acattaacgc gcgaagaacg tccataccaa
    15241 atcatcgaag cagtaggtgg tttcgacaat atcgaagatg tggatgcctg cgcaacacgc
    15301 ctaaggctgg cgctggtaga cgataagaaa gtgaatgaaa aacggctgaa ggagttgggg
    15361 gctgcgggtt tagtgaaact gggtgatggc ggtgtacagg tgatatttgg cggcaaatcg
    15421 caaatcctgc gcgacgaaat caaaaccgta atgagcagac cgcgcccgac agaaatgaca
    15481 gtggcctgtg ctaactaatt cgagattagc gggctggttt cagcccgcca gacagagaga
    15541 gctgaagtat gaattcacaa ttactggcta atgccatccg aatgttaagt gttgatgcga
    15601 tccagaaagc gaactccggt catccgggcg cgccgatggg gatggcggat attgccgaag
    15661 tggtctggcg tcgtcacctg cgtcataacc caaaaaatcc gcagtggttt aaccgcgacc
    15721 gctatgttca gtcgaacggt cacggctcca tgttgattta cgctttgctg catcttacag
    15781 gctacgacct gtcgatggat gatattcgcg actttcgtca gctccactcc cgcacgccgg
    15841 gtcatccaga atatggttat acgccgggtg ttgaaaccac caccggcccg ttggggcagg
    15901 gcgtagctaa tgcggtgggg atggcgattg cggagaaggc gctggcggca gaatttaaca
    15961 aaccgggctt taatattgtt gatcatcaca catggttatt tttgggtgat ggctgtctga
    16021 tggaagggat ctcccacgag gcctgtggtc ttgcaggaac gctgaagctc ggcaatctga
    16081 ttgctatctg ggacgataac ggtatttcga tcgatggtca cgtagaaggc tggttcgctg
    16141 aagatacggc tgcccgtttt cgtgcctacg gctggcatgt cattgaaggc gttgatggtc
    16201 acgatccaga agcggtagat gctgcggtgc gtgaagcgaa aagcgtcacg gataagccca
    16261 gtctgctgtg ctgcaaaacg attatcggtt ttggttcgcc aaataaagcc aacagccacg
    16321 attgccacgg ctcggcgctg ggtgcagatg aagtggcgct ggtgcgtgaa cgtctgcaat
    16381 ggccgtatgc ccctttcgaa attcctggtg aaatttatgc cgaatgggat gcgaccgaga
    16441 aaggcgcgca ggttcaacag gaatgggatg cactgtttgc cgactacgcg aagcagtggc
    16501 cggaactggc ggcggagttt actcgtcgca tgaagggtga tttgcctgct ggctgggtcg
    16561 agaatatgca gaaatatgtg cacgatctgc aatcccatcc ggcggcgctg gcaacccgtc
    16621 aggttagcca gaaatgcctc aatcacttcg cagatatgct gccggaactg atgggcggct
    16681 cggcggactt gtcgccctct aacctgactc gccatcagaa atcggttgat ttcaccggag
    16741 aaaatccggc ggggaattac atctcctatg gcgtgcgtga atttggtatg tcggccatca
    16801 tgaacggtct tgcgctgcat ggtgggttta tcccttacgg cggtactttc ctgatgttta
    16861 tggaatatgc gcgtaatgca ctgcgcatgg cggcgctgat gaagatccgc tcggtgtttg
    16921 tttataccca cgataccatc ggcctgggcg aagacggccc gacacatcag cccgttgagc
    16981 aactggcgtc gctgcgtctg acgccgaata tggaaacctg gcgcggttgc gatcaggtgg
    17041 aagtggcggt ggcctggcaa caggcgattg agcgcaaaga tggcccaacg tcgttggtat
    17101 taacgcgcca acctctggcg caacagccgc gcaccgctgc acagttggca gagattgcgc
    17161 gtggtggtta tgtactgagt gattgtgacg gtcagcctga gatgatcctc atttctgcgg
    17221 gttcagagat tgagttagtg gtcagcgccg caaaagcgtt aacggaagaa gggcgtaaag
    17281 ttcgcgtggt ttccatgccg tgtaccgagc gtttcgataa tcaggatgcg gcgtacaaag
    17341 agtcggtatt gccaaaagct gtacgcaaac gtctggcggt agaagccagc atcgccggat
    17401 tctgggaacg ctacgtgggc ctggacggta aagtgatcgg catgacgagc tttggtgagt
    17461 ctgctccggc gaatgtgttg ttcaaacact ttggttttac gccggaaaat gtcctggcgc
    17521 aggcgcgtga gctactgaac agctaaacct gcacaagggg tgaaaggatg accttttacc
    17581 ccttgaaacg ggagccaaga aactcttcac gggtgcggta tttctcgtaa tgcgccttgt
    17641 ttttatacat aaacaacagc accagcatat caataacgcc cattgccgga acgcgagtgg
    17701 agtcgaaggc ttcaaagaag attttatcgc tgaccgcggt attgagttga tgggtggaga
    17761 gttttgataa cggagattta atatagttag tgatacaaac ggatttggca ccgcgctctt
    17821 tcaccacttt aaagatatcg accattaagt tggtgctgcc agagttagaa ataccaatcg
    17881 ccaggtcgcg tttccccata gaatatgccg cattaatttg catttccggg ctgacgtgaa
    17941 caacggtttt taatccgagt tggctgaatt tataagcact ataaacggcc agtgggtttg
    18001 aatttccggc agcaaaaaag aaaatagtat cgcaattagc aagtagtttg agaatggact
    18061 caagcacgtc ggcattaaaa aattgcgttg attgtattaa atcttcggcc tgttttttta
    18121 ataaaaaggt tgccaaagat gttatatcgc tgaagtcggt attttcagga tagctattgt
    18181 caggattaat gacttctttt gctaatgcaa tttttaaatg gtaataaccc tgataaccta
    18241 atttcttaca ggtgcgtacg atagtcgctt cactggcatg gcttttttct gatagttcag
    18301 tgacactata gttcacgact tcatgtgcgt ggctaagaat atagtcagcg accttttttt
    18361 ccgcctgatg cagtgtaggg taattgcttt gaatcagact ttttacggtt atgttattca
    18421 taattagtaa atttcattct gacagaaagt gctgattata acatagtttt caggcgtgga
    18481 taggtgtgaa aaaatgaagg tgtgcagtga acgtaatgat taacagaccg gttaattttc
    18541 atgaattacg tcaactgcac aaaaataatt taaacgggcg ctctaattaa agcaagactt
    18601 atatttttat cattgtattg tcaattaaat acaaatcgtg tcattaattg ctggtttctt
    18661 aaatattcta tgcaccactg acaggctttg cccataccat cggaacgagc ggcaagaaat
    18721 aacttggtgg cgtgtttatg ttcgcgcatt ggctttttaa gcaatttacc gctgttcaat
    18781 aacggtaatg ccagatgatg tggaatataa ccaataccga cgttttgttc tattaaggcg
    18841 attgccgtag cgaagtcagg aacaaaaatg ggtttttgtc cttccaacaa ccaggcttgc
    18901 atcgggggga agttaacgga ggtatcgcgg atacaaaccg ctggataatg acgcaattca
    18961 ctgttttcta atggatgccg cttcgctgcc agtgggtgga gtgggctgac gacaaaatcc
    19021 cattccattt gtccgaccgg ttcgctaatg atgccttcac tgcttggtac tgcatgagga
    19081 gcaccgtaga ccagatcagc acgcttactg tacagcgcat cccagcagcc gttgtatacc
    19141 tcaagatcta ccgtcagtgt tgttgatgtg aattgccgtt caaaatctcg aataaatgcc
    19201 accagcaaat caccaggaat aatattgttt accgcaattg tcagttcacg ctcaatacca
    19261 tcgtgaatca gcactgtgtt gcgctttaat gcttccaggt cgccaagaat agttttgcta
    19321 tgttcgatga agtaacttcc cgctggcgat aattcaatat agcgtccctt acgaatgaac
    19381 agttcaacgc ccaggctctc ttccgctttc ttgatggtgt aactgatggc ggaagggact
    19441 ttatttaact gctcggctgc ggtggtaatg ctctggtgtc tggcgaccag atgaataaca
    19501 cgaagcgttt cgtctgagat gaacataagg ctccctggtc agtggtaaag tggctgaatc
    19561 tctccagatt cagccgtaat cattgcctga gtatacccag gaattagcgg tgttgacttg
    19621 cccgactgag aatcgcatac gcgatacatg ccaccagaat cgagtctacc gaagggatcg
    19681 tcgtcagggt gaataagcct tttacggtca taaagccaac cgctgcgcct atgatccatg
    19741 ctgcgaatgt cagcacttta atctgtgact cctgggctaa cactgactct tgatagccat
    19801 tacggcgata aaggaaaaag tcagcgatat aaattccggc aacgggtggc gtggcgatac
    19861 ccaggaacag cagaaacgga ataaaccacg ccataatatc catactgcct acgatggcgg
    19921 aaaggatacc cagcgccacg gtaatctgcc atttgggaaa gcgggtaagc agtgtggaaa
    19981 ccaccagcgt gccctggaac atattgcccg cgttaccggt gacgcaggcg aaaatcaaca
    20041 acagcgccgc aggcagaact gcaccgaata ccgccatcgc acccagtaaa gatccctgac
    20101 cactaatggc gctcggcgtt gcacccgccc agtagagcag agggtaggca accagaaacg
    20161 tcacgcctgc ggcaatcagc gcatgtttgc ggttatgcac aaaactaccg aaatcaggca
    20221 gtgtggcaac cagcacaata atggtgccaa caacggtgga aacggcgaca cctgtattca
    20281 tctccacaat cgccgctggt actgccactt cggtatgggt tgccacatag aggatgtaac
    20341 acagcaccag cgcgataacc ggcacggcaa cctgggcaat ttttcccaga acggcaaagc
    20401 caaatgccgt agaggcgaca aaaatgacac agccagccgc cactaacagt gccagtggaa
    20461 cttccagtcc atgttgagcc agtaagtcat gtaccgaatg accaaacata ttggcggtaa
    20521 cggcaatcca gccgaacaga cttatcgcca tcaaaatgtt aatggcgatg gccccttttt
    20581 cgccaaacga atatttcaca atgccatacg ttggcaggcg ggctttttct cccacgctaa
    20641 tggtgatggc tgaaagtaag gtcagaatga cgccgcctaa caatacgacg ctaattaaac
    20701 tgccgctgga aagttgattg ccgaggcttg aggatgacaa cagcacaggt gtgacggcaa
    20761 tacccagcaa aatcatggca atacgcaatc ctgatgccgt attcagattc tcttcttttt
    20821 tacgcatagt atgttccctt tataaatgcg cgttacacag tatgaaaatg cccggtgtat
    20881 ttacgccagc tacccagttc tgtaatttcc cgttggcctt cggtcagcca gttttctgcc
    20941 agtacgccca gcacttcgct accatcggca agtttgattt tgccaattgc cagcccagcg
    21001 ggttcgctca ttaacagagc ggcaaaactg gccagcggta attgccaaat ttctacgtcg
    21061 acgtgtgttc ctccttcatt cacgcgaatc attcccggat ggcgatcatt aatactccac
    21121 aaccggtaat gggcgtcagt tcggtcttca cggacaaaaa taccgcccgc tttttgcata
    21181 tttgggttga gttccaggcc gcgcatcagc gttccgttaa cggctaaaag cgttgtagtc
    21241 attgtgttct ccagagaatt aaagggttat ccatgtaccg ctctgatgcg ccagggctgc
    21301 ttttatggtt tgcggtgaag taatcaggcc gctggcctgt ttgccttgtt gctggctggc
    21361 attgatgaaa tcaacaatgg cttccacttt gggttgcata ctgccgacac caaactgacc
    21421 ttcccgcaac agcgtttgcg cttcggcaac gctgatagcg tcgagccact gttgttgtgg
    21481 tgtaccaaag ttaatcgcca ctttttctac accggtgggg atcaccagca gatctgcgcc
    21541 taactgactg gcgagcagcg cggaggccag atctttatcg atcacggctt ctacaccttt
    21601 cagttgttgc ccgtctcgca ccaccggaat accgccgccg ccgcaggcaa taaccagata
    21661 tccctggcgc accaggtgag cgatggtgtc gtgctcaata atttccagtg gtgcaggaga
    21721 gggaactgta cgccgccaac cacgcccggc gtcctccatc agcgtccagc cgagttgttg
    21781 ttggcgttgt tgggccgttg cttcatcgag aaacgcgcca atcggtttac tggggctggc
    21841 gaaagcatcg tcatgtgggc tgactcgcgt ttgtgtcacc agggcaatta ccggtttgtt
    21901 tatgccacgg cgagcgagtt cgttatgcag cgctttctgg aacatgtagc caattgcacc
    21961 ttgtgtatca cccacggcgt aatcaagtgg aaccggagaa acttcgttac tggcgagttc
    22021 agagcggcgc agaataaagc ccacctgcgg gccattaccg tgggttagca ccaggtccca
    22081 tccggcttca accatatcaa cgatatgctg cacgctttcc atcactgcaa catattgatc
    22141 gggaatacta ttgcgttgct cgtcctgaat gagcgcattg ccgccaaccg cgacgacagc
    22201 cagtggctta accatgatgg ttctcctttg cgacgatctc cgccagtgca cgtacagcgg
    22261 cgacaaaaca ggccatcggc gcagtggtga tcccggcacc aatttgcccg acccccgctt
    22321 gtttgtgggc gatacccgta ttgatgaccg gtaaaatgcc gcgatcggtc actttacgcg
    22381 catcaatccc tgctgcggtg ggggcaaaat tgagggctgg aagtgtaaat gcggggttac
    22441 cgccgagggt gatggcttgc atccgacggc tgttattggt ggcatcggca ggggtgcctc
    22501 ccacaaattt gacgatcgcc ggagacgacg ccatcgcgaa gccacccaca cctgcggttt
    22561 cagtaattgc gctatcaccc aggtcagcag cagcgtcgtc aacgccaaat ccggggaaga
    22621 acagaccttc aacggcattg gcaggagcct ggaaccagcg atcgccagta ccggaaatcc
    22681 gaataccaaa attcaccccg ttacgcgcca tcaccgtaac cattgaactg aacggtacat
    22741 tggcggcggc atccatcatt gctttacagg cggccatcga cagattgagg aagaagtgat
    22801 cgttaccggt gataaatgcc acgacgcgct ggatatgttc ctgtggaaga gagcaggcca
    22861 gcaatgccgg gagcaggcgc ttaatcagca aaccggtagc ggcagcattg cggttatgaa
    22921 cttcatcccc catatgcagc gcctgcgcca ttaatggctt gagttccagc tcgccgtgtt
    22981 gtgcgattgc tgctttcatt gccggcgtca gttcatcgcg catccaggcc agacgattga
    23041 gaacttcatc gttattggcg ccaaaacgga gaaccttacc cagaccttca ttgaaattgc
    23101 taaaagtacg gtggccgttg gttttgtttt ctaccaccca taacggcatc gacgggctga
    23161 tgatgcctgc cattgggcca accgcgtgat aatgatgaca aggggcaagg tcgatttcgc
    23221 ctgcgtttat ctggttttca gcgtcttgct ggctggtggc ccaaccttcg tacagcatgg
    23281 caccaataat cgcccctttt accggaccac acatctcttc ccaggcaatc ggcgggccag
    23341 agtgcaaaat acgtttttgt tcagccattg ctggaatggc ctctttagca agcatgacat
    23401 ccaccagcac gggctgtgct tcgagataac gtgccatggc gatttggttg gcattttcta
    23461 ccaggggatg acgtagcaac gatgccagat ccaatccagc gtcaatatcg ccttgtgctg
    23521 gtggctgcca gttgagtgca attgcgcttc ccccggcatg ttgaatattg tcagcaaaac
    23581 tgtgtagtcc cgcgttcaca accttcaaag gctgattaaa taaggtattc atgcgtttag
    23641 cccctttttc tgcaactgag tctgtgcaac ggtgctggcc catagcgccg cctgtgcatt
    23701 gctgctggcg atgatgaccc ctgcattttg tagtgcgctt atctgctgac tgcgtatttg
    23761 cggatcagct tccgttccgc aaacgtgcgc aatcagcaac ggagcgttat tcatgtcgat
    23821 gtgtgaaagt tggtccagta attcgctggc aggcgtcgtg gaagcgccat aaccaagtac
    23881 caggtcgaat aacacgacgg cagtgtgtga gtcgttgagt tcgttgagca gacgttgatt
    23941 gcgcagagtc gggtcgatca ttggatgcgg tttgccacgg gtgaaatcat cgtcgcccat
    24001 atcgatcaac gtgtgatctt cgctttgcca gatatttgcc agagcacgat tacccgcaac
    24061 tggcgtattg gaagctgcgc tgaatccctt ttgttggcag ataagttgcg cttcgtagca
    24121 gaaggttcct ccggcgaaaa caccgcgtat cgcctggcga tgggctggca ggcgctggca
    24181 ggcgttttgt aacattgtga gatcgtcgca gggtacatcg gtttcgacag ccggaagtgg
    24241 ctgagcattc agtaacgcca cagcgatgtc ggcggcgctg gcaagtgtgg tggcggcagt
    24301 gatattggga cgtgccagat catgcggatt cgccccgagg aaattcacca ccacgggttt
    24361 tccacaagcc tcggcgcgct ccagaatagt ccgggcaacg atcggtgaag gcggttttga
    24421 aatcagcaca attacgcgag tttcatcgtc ttgcgccagc gcatctaaag cgaagagcat
    24481 cgatattcca ccaatctctt tactgagatc atgtccgcct gttcccaacg cctgagaaat
    24541 acccgctccg agctggtcga ttcggcaagt cacttcctgt agcccggtgc ctgacgcgcc
    24601 aataacacca attgctccgc gtttcaccac attagcgaag cctaacggaa tgccgttaac
    24661 aatggcggtt ccacagtcag gtcccattac gatgcgctga cgttctcggg caagcgtttt
    24721 gatggatttt tcctgcacga tactgacgtt atcgctgaac atcatcacgt tcattccgag
    24781 gtttaacgct ttaattgctt cggcggcggc gtagtctccc ggtacagaaa tgagtgccag
    24841 gttggcttcg ggttcggctt ccagtgccat ttccaggctg accttttccg gtgttttaat
    24901 gccactatcc gtttcatcat cgggtttgct ggtcagacgt tgctgggcca gtatcagtgc
    24961 ttcattgcaa atatcttctt cacccatgac tgcaatcact aaatcattgg gactggcgtt
    25021 gatttcgtta cccagacccg catcgcgcaa ttgttcgaga ttattgggcg ttcccatgac
    25081 gaccgaggcc tgttgaatac cgggcaactt gctgatttgc gcggaaatct gcatcagaga
    25141 aactgagtcc tgataaagat tactgaaaac tttatattta actttcatgc tgttacgcct
    25201 gtttcattcc gcccgatagc aaaattcggg caaagcatcc ccgtgtagcc ccataaacgg
    25261 gctacgggag aaaaggttga aatcaattca tggtgttcag ggttcgaaag ccatgcagca
    25321 ttcctgcaag gcaatctact cctgacgttg cgccaaattg catgacctgt tctgcacagg
    25381 atgcgattgt cattgccgat gcgctcccaa ccagttgtgc gagtaactgg caaatcggtt
    25441 ctgaaaaatg tccctgaagc gcacgctcca gataatgacg actgatatcc gtggtgttgt
    25501 gcgcatgttg atcaattgcc tgctgcaacc ggtattgatg atcggcaagt ggtgcaggga
    25561 gttgccataa ccagagcgct gccagatagc ccaacaaata gtcatctcca tcgggcgtta
    25621 agcctttacc aaagccgatc agttgtggca cctggcgttc cagcgcatct ggatgctcca
    25681 taatcgctaa tccctggtgc gtgagactat ccggcaggtg caatgtgctt tgaacgttat
    25741 gctccagaca aaataaaagc cgttgcgatg ccatcgttgg gtagtgctgg aaaaccaaag
    25801 ggaatgcctc aagtgtcagc attcccccca caaatggcga ctgccagagc gttgcgttat
    25861 cgagcgccac acaaaatcga ggtgtccgca gcaagcctgg ggttaactga atgggatctg
    25921 aatgggcaat ctctcttcgc cagtcccaac attcaggtag ccagactctg gcagcatcag
    25981 ggagattcag atacgtttgc gcacacaaca gagtgatgaa tgttcccgcg tctgaataca
    26041 gatttaccgc atgtttaaaa gcgctatgta gacgtaaatc ttgcgtttca tcctgcggga
    26101 aaagatatcc caacgacgat gcgcaaagcg taacctgtac catcggcgtt agccttccag
    26161 acctgcaata atggctttgg aatcagtaac ccaaccgaaa atggcacctt gtgctttgat
    26221 catctcgagg gcgtatttct ggaattccgg gaaataggag ccgacacaat cttccgggat
    26281 aatgcattca tagccacgat catttgcttc gcgcacggtg gtggttacgc aaacttcggt
    26341 tgttacacca caaactatca gcgtcttgat accgtggttt tgcaaaatca gatgtaagtc
    26401 ggtttgataa aacgcgcctt tgccaggctt atcaatcacc ggttcgcctg ccaccgggta
    26461 cagttcagga atgatgtcat gacctgcttc acctctgacc agaatgcgtc ccatcggtcc
    26521 tggttcacca ataaaggttt ttccgccgcg cgtcagtttg gctggcgggc agtcgcttaa
    26581 gtctgcacga tggccttcac gagtatgaat aaccataatt cctttttgac gtgcggctgc
    26641 cagcacctct gtacatggtg cgatagccgt gcgtacccgt gaaacatcat tgcccagtgc
    26701 ctcaccgaag cccccggctt cgacaaagtc acgctgcatg tcgatcatga ccagtgcagt
    26761 tgtgcgcgga tcaaaaggta attcaaaggg ttgagcctga aaaatggatt gcgtcatggt
    26821 aaatctcctg tctgatgttt aaacgatcca gttgttctga gaacgagtat tcaggagaaa
    26881 agcggcacaa aacaggaaaa aagtttgccg cacagcaacg aaatttttgc attcagcagg
    26941 tggtgtgatg acgaatgtga agggagtcaa agagtggcat acagcaacaa attttttgat
    27001 gctgtatggt tttgagatta aaaaaatcgt tgaaattgtg tgagcttaat catcagattt
    27061 agcgactatg gcgttacttt ttaacaccat actgtgtaaa ccattccaac attcgctgcc
    27121 agccgtcttt cgcagactcg gcatgatagc tcggacgata atcggcgttg aacgcatgct
    27181 ctgcatccgg gtacacgata atctctgctt tcgcgtttgc tgcccgcagc gcctggcgca
    27241 tcgtttccac actctcttgc ggaatgctgt tatcctggcc gccatataag ccgagaaccg
    27301 gcgcgttaag atcggttgcg atatcaacag gttgtttcgg tgaattcagc gacttatcgc
    27361 ccgtcagttt gccgtaccac gccactgcgg cttttagctg tggattatgc gcggcataca
    27421 gccaggtgat acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc
    27481 cgttgcggga agcccaactg gcgacatgat cgagatcggc cagcacctgc gagtcaggca
    27541 cttttgctac cagaccgcca agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt
    27601 cgcggaagta aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa
    27661 tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac tacaatgacc actggcagtg
    27721 ggccatcgct ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga
    27781 tagaggtgaa gcccgccaca atcgcgtcgt ccggagtctg aacgatggtc gaagcgagag
    27841 gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct
    27901 tataaaaatg ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat
    27961 cggctcatct tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tgcatcactt
    28021 ttgatgggta aatttatgca acacattttc gtcatggtga tgagtatcac gaaaaaatgt
    28081 taaacccttc ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt
    28141 gtatatgtcc aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc
    28201 tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga
    28261 taagccggtt aagctggcat ctcaccgcga attcaccacc tggcgtgcag agctggatgg
    28321 taaacctgtt atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga
    28381 agagctggca cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca
    28441 gccgcatatt aatgtgggtg atgtcctggt taccacggcg tctgttcgtc tggatggcgc
    28501 gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc
    28561 gctggttgaa gctgcgaaat ccattggcgc gacgactcac gttggcgtga cagcttcttc
    28621 tgataccttc tacccaggtc aggaacgtta cgatacttac tctggtcgcg tagttcgtca
    28681 ctttaaaggt tctatggaag agtggcaggc gatgggcgta atgaactatg aaatggaatc
    28741 tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggggt
    28801 tatcgttaac cgcacccagc aagagatccc gaatgctgag acgatgaaac aaaccgaaag
    28861 ccatgcggtg aaaatcgtgg tggaagcggc gcgtcgtctg ctgaaataaa aaaacgctcc
    28921 ccaacttcgg ggagcgatat cagttattga cgccgtaaac gcattatttt gcggattatt
    28981 ggcgtcagta gcgtaaaggc tgccagagtc agcaatgcca gagtgatcgg tcgggtatac
    29041 agaaagtcga gtgacccacc agaaagcgac agcgcacggc gaaaattact ttccgccatt
    29101 ggccccataa tcaatgccag cactacgggt gatgccggat atcctccttt ctgcatgaaa
    29161 tagcccacca cgcctgcaat cagcataatg ccaacatcaa aaaggctatt gttcaacgca
    29221 taagtcccga ccacacaaag tgcgaggatc atcggtgtga gaatattgcc gggaattttc
    29281 accacattac cgatgacttt caaagagagc agtccaagaa ctagtagcat gaagtaacag
    29341 aagatcatgc cgacaaacag tgtataaacc atatcgccgt ggtcggtaaa catcagcggg
    29401 cctggttgca ttccctgtaa cgttaacgcg cccagcatga tggcggttac cgcatcgcca
    29461 gggatcccca gcgtcagcat aggtaacaat gcgccaccgg tgcagccatt cgcgcctgcc
    29521 tcacaggagg ccacagcttt aatttcaccc ttaccaaagt tttctggtgt cttactgaaa
    29581 cgacgagttt cgttataggc aacaaaagct gcaatatccg cccccgcacc ggggatcatg
    29641 ccgataaaaa cccctaatcc tgatgagcgc aaaatggtta atgcgatgcg gcgtagtgtt
    29701 tgccagggca gtaacagact gccgatagcc accttcgccc cctgacgaat ttgctggttt
    29761 tgctccatag agcggaatgc ttctgaagca gcaaataaac caatcatcac cgggataaat
    29821 ggcacattca tcagctccac aaatccacct gtaaaccgtg gaaaaccgcc cattggatca
    29881 aggcctatcg ttgcgatcag caatccgcca accccggcaa tcagcccctt aatcagcgac
    29941 tcaccggaaa tactggcaat aatgctcagt ccgaaggttg ccagggcgaa ggactccgag
    30001 gcactaaatt tcagcgccca actggcaagt accggagaga gaaacatcag cacgataatg
    30061 ctgagcgttc cgccactaaa ggaagagaac gtggcggcac tcaatgccag ccctgctttt
    30121 ccctgcttcg tcagctcata tccgtcaatg gcggtggctg cggcggcagg cgtaccggga
    30181 attttgagca aaattgcggt gatagaaccg ccatagacgc cgccaaaaaa gacgccgcaa
    30241 atcatcaaca agccagaaac aggctccatg ccgtaggtga aaggcagcaa aatcgccaca
    30301 cccatggtcg ctgtcagacc aggcagagcg ccaatcacaa tacccagcat caccccaaaa
    30361 gtggcaaaca gcagcgcctg aggattattg agtaatgtcg aaaaaccctg agttaaaagt
    30421 tcactttcaa acattgctgg cccctgttat tcgaaaaatg tgccgacagg caatggcacc
    30481 tgtaacgctt cgacaaacag caaccagagg attgccgtta acagcagcgc aatacccggt
    30541 gtgagtaccc atcgcctgta gcccatcaac gtcataatga cgatgaggaa aacaggagta
    30601 gtaatgagat aaccgcagta gctcatcgcc acaatataaa acgcggttgc cgcaattcct
    30661 gttgccgttt tcctgaatgc cggtttttcc tgtgcgttgg cgcaagaggc tggttttgcc
    30721 tgcaaaagat cgcgtatcac cagcaagaca gcgagcacaa tcagggcaca ggcatagaag
    30781 gtagggaagg cacttgcacc gggatccgtt tcaatggcag ttgctggaaa atcagcggca
    30841 ttaatcatca ccgcgccagc aaccacacaa agtaacagcg ccaccaccat cgttgatgaa
    30901 aggcgcttgc ggtgcggtac ggcgagttga gcaacagact gttttgtgtt catcaaccgt
    30961 acctcaggtt tattttttca ggcccaggcg agtcagcaac tcgtcaaagt acttttcctg
    31021 ttcgctgatt tgggtctgga agctggcagc gtcaagccat gcatagttga gattcaactt
    31081 ttgtagtgca tcctggaatg caggctcttc agccgtctct tttgctgcct ttgccagaac
    31141 atccaccaca tcctgcggcg ttttttgcga cacaatcagg ccgcgccagg tgccgatgga
    31201 gagatcaacg cctttctctt ttaacgtcgg gacgtcaggc atggttttca ttcgctcatc
    31261 cgccatcact accagtgtct tcagcttgcc gcctttcaca tggttgataa cttctcctgg
    31321 gcttacggaa accgcttcaa tgtgcccgcc taacaggcct gtaatggcag gggctgcgcc
    31381 gtcatacggg acatgagaaa actttgcgcc cgttttgtct tccagtgcag ctgccgccag
    31441 atgccagata gctccggtgc ctgagttacc aatgcgtact tttccgggat tcgctttggc
    31501 gtaagtcata aattcttcat agctattcca cggcgcatcg gcacggactg tgatagcagc
    31561 cggatccgca ttcagacggg caatgggttt aaagtcgctg gttttaaaac gcaccattcc
    31621 gaggctggga agggtggtca gttcaaccgt gcctaaacca attttgtaac cgccagggcg
    31681 ggcagcggcg atttcactca ggccgatagc accgcctccg ccaggtttat tgataacccc
    31741 gatgctgacg ggtaaatggt ttttggcggc atcagcaaag gcacgggcaa ccagatccgt
    31801 accgcctccg gcagcgtagg gaacgactaa ctcgatctgt ttggttggcc aggaggcagc
    31861 ctgtacaggc gcaatgctga gggcagcgcc tgcaagaagt accattgcag ccattttttt
    31921 cggtaaggta agatgtttta tcattgcttt tatatcctta tttatcagtt agttgctatt
    31981 taaattgttc catgcgttca taaacagtac tgcattttca tagacttgct ctggcgtcat
    32041 gccggggtga tatagcgcag aaccgaggcc aaagccgttt gcgccgtggc tcagataacg
    32101 cgccatggtt tccggtgtga taccgccaac cggtaacatc ggaacggatt gtggaataac
    32161 cgcacgccag gctttggtaa cttctggcgt aatttgttca gccgggaaca gcttgatggc
    32221 attagcacca tgttcaatgg cagtaaaggc ttcggtgggc gtggcgacgc caggcgcaca
    32281 atacataccc agcgcacggg cgcgacggat caccgcagta tcgcaatgcg gcatcacgat
    32341 aatctggccc ccagcctctt taaccgcaac cacctgctct accgaaagca cagttccggc
    32401 tccgaccagg cagtccttag gcagggcgtc gcgcagtaaa ctgatacttt gcaacgcctc
    32461 cggcgagttc ataggaatct caatcaggcg gaatccggcg gcgtacagtt tttcaccgat
    32521 ggcgacaatc tcatccggtt tcacgccgcg taaaatggcg actaacccgg ttttttgcaa
    32581 aagcgtatta aatggcatgg gtacattccc tggcgttggt ggacagtaac ccggcagcgt
    32641 gggcgattcg ccataatccc tgttcagtgg cattaggaat ggcgtgtatc tgtctgtcgg
    32701 gaaaaacgtg gctgaatgct tgtgaatagc ggctgttcag atttgcgcta ccgattaagg
    32761 cgatcggtgt tgtggcgggt aaatcacgta gcaaactgga tgccagacca cataattcat
    32821 ggcctatcag taatccggaa aggtaatccc cctgcgagga cgacgtaaga ttactggtca
    32881 gcaggcgcga acgggtcgaa aacagcgtcg ataataatcc ggtctgatga tgctcttgtg
    32941 ctgctgtcag cccgtgggtg aaggcatccc agtgcagttc atctccagga tgcatggtgg
    33001 caccgagaat cgaatggcgg gataaaacat caaataactc gcccgtcata aaggttcgga
    33061 aatcggtaat ggtgttgttt tcaactactg cccatttcgc atgtgtgccg ggtaagccta
    33121 tcagcactgg cataccctga tggatcgcat tttgcaggga cggttccatc gagatagcac
    33181 cgaagatttg tgtctcttcg ccacgcatca cttcgggcat ttcaccttct ttaatcacgc
    33241 caggaatgat gtgcagcatt gtctgctgct ggtggataac aggcgtcaac tgcttcgcca
    33301 gaccctcgag agtgaccggg caaggtaaat aagctgctgg ttgccaccct tgcgcactgc
    33361 cgagcatgcc gcaagccagt accgggcatg gggtttgtgc cagccagtcg ccgcagacac
    33421 gtaaaaacgt atcgtggaaa gcgtcctcac gtgttgctgc ctgggagggt aatttcatga
    33481 tcccccaggg gaattcacgt ttttcttgtg gatttggtga ttcgccaaaa cgccatgccc
    33541 gaagcgatga cgttccccag tccagggcaa taaatgaggg tgctcccggc attagtcgtt
    33601 tcctttaatc atggtttatt tatctttgcg gacgtgctta ccccagccca gttcagcgga
    33661 tatctcttct gcacaggatt tgacgtaagg tgccagttct tccagacgcg ccagcgacat
    33721 atacgtggtg gtactggcga cagaaatagc cgccacaatt tcatcccggg cgttgtaaac
    33781 cggcgctgca acgcaacgga tagtcggctc gttctcttcc agatcaaaag caaaaccact
    33841 ggcggcataa cgacgcatgt tctgaataaa tgcgcccagt tttgtttcat caccacaggt
    33901 cttaaacagc gtgcgccact cttcttctgt tcggttgagg atcaatgcct tgccgatgcc
    33961 ggtgattgcc agtggcatgc gatggcctgg gcgtgagcgc atttccagcc cgcgctgact
    34021 gttgatcttc tccagataca gcacttcgtc gccttcaacg atccccagat gcacggtgtc
    34081 cagggtgtaa cgggcaaggc gttgcaaaac gggctgggca atttcgtaca gcgaggtgct
    34141 ttccagtgct ctggcaccga attcgatcag tttggcaccc agttggtagc cctgcgttgg
    34201 gacatggcgc aggtagcgtt gttccaccag aacggtgact aaacgatggg ttgtgctccg
    34261 ggtcgtgccg gtgaactcgc cgatactttt taggtcatgg cagccgttat aaacagcatt
    34321 aagaaccgcc agtccacgta ataacgtttg tgcgcctgcg gtggttgaag aggtttctgc
    34381 catacgtttc tcctgatgat attgctgaag tgttccacat aatgacgtgg ggaaaaggtg
    34441 atatatccca caatataaac ttcatctcat attatgggat aaaataatga aaattaacga
    34501 tcgttttgcg tagcgcctcg caggaaactt ctttcaaact ggacgtttgt acagcacaat
    34561 tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc aatcatggtt
    34621 tacgcagtca ttgcgttggt gggtgtggca attggctggc tgtttgccag ttatcaacat
    34681 gcgcagcaaa aagccgagca attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg
    34741 gcaaaacaac aaattaccca aagcgagcac tggcgcgcag agtgtgaatt gctcaataac
    34801 gaagtgcgca gcctgcaaag tattaacacc tcgctggagg ccgatctgcg tgaagtgacc
    34861 acgcggatgg aagccgcaca gcaacacgct gacgataaaa ttcgccagat gatcaacagc
    34921 gaacagcgcc tcagtgagca atttgaaaac cttgccaacc gtatttttga acacagtaat
    34981 cgtcgggttg atgagcaaaa tcgccagagt ctgaacagtc ttttgtcgcc gttgcgtgaa
    35041 caactggacg gttttcgccg tcaggttcag gacagtttcg gtaaagaagc acaagaacgt
    35101 cataccctga ctcacgaaat ccgcaatctc cagcaactca acgcgcaaat ggcccaggaa
    35161 gcgatcaacc tgacgcgtgc gctgaaaggc gacaataaaa cccagggcaa ctggggcgaa
    35221 gtggtgctaa cccgcgtact ggaggcgtct ggtctgcgtg aagggtatga atatcagact
    35281 caagtgagta tcgaaaccga cactcgttcg cgaatgcaac cggatgtcat cgtgcggctg
    35341 ccgcagggca aagatgtggt gatcgacgcc aaaatgacgc tggttgccta tgaacgttat
    35401 ttcaacgctg aagatgacta cacacgtgaa agcgcgctac aggaacatat cgcgtcggtg
    35461 cgtaaccata tccgtttgct gggacgcaaa gattatcaac agctgccggg gctgcgaact
    35521 ctggattacg tgctgatgtt tattcccgtt gaacccgctt ttttactggc gcttgaccgt
    35581 cagccggagc tgatcaccga agcgttgaaa aacaatatca tgctggttag cccgactacg
    35641 ctgctggtgg cgctgcgcac tatcgccaac ctgtggcgtt atgagcatca aagccgcaac
    35701 gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt gttcatcgat
    35761 gacatgtccg cgattggtca aaatctcgac aaagcgcagg ataactatcg ccaggcaatg
    35821 aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta
    35881 ggggtagaaa ttaaacgcga gattaatccg gatttggctg aacaggcggt gagccaggat
    35941 gaagagtatc gacttcggtc ggttccggag cagccgaatg atgaagctta tcaacgcgat
    36001 gatgaatata atcagcagtc gcgctagccc attgggggta gttaagccgg gtagaaatct
    36061 agggcatcga cgcccaatct gttacacttc tggaacaatt ttttgatgag caggcattga
    36121 gatggtggat aagtcacaag aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca
    36181 aaaagcggat atggtcgccc acgttttcca ttccgtggca tcaaaatacg atgtcatgaa
    36241 tgatttgatg tcattcggta ttcatcgttt gtggaagcga ttcacgattg attgcagcgg
    36301 cgtacgccgt gggcagaccg tgctggatct ggctggtggc accggcgacc tgacagcgaa
    36361 attctctcgc ctggtcggag aaactggcaa agtggtcctt gctgatatca atgaatccat
    36421 gctcaaaatg ggccgcgaga agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt
    36481 tcaggcgaac gctgaggcgc tgccgttccc ggataacacc tttgattgca tcaccatttc
    36541 gtttggtctg cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct
    36601 gaaacccggc ggccgcctgc tggtgcttga gttctcgaag ccaattatcg agccgctgag
    36661 caaagcctat gatgcatact ccttccatgt gctgccgcgt attggctcac tggtcgcgaa
    36721 cgacgccgac agctaccgtt atctggcaga atccatccgt atgcatcccg atcaggatac
    36781 cctgaaaacc atgatgcagg atgccggatt cgaaagtgtt gactactaca atctgacggc
    36841 aggggttgtg gcgctgcatc gtggttataa gttctgacag gagaccggaa atgcctttta
    36901 aacctttagt gacggcagga attgaaagtc tgctcaacac cttcctgtat cgctcacccg
    36961 cgctgaaaac ggcccgctcg cgtctgttgg gcaaagtgct gcgcgtggag gtaaaaggct
    37021 tttcgacgtc attgattctg gtgttcagcg aacgccaggt cgatgtactg ggcgaatggg
    37081 caggcgatgc tgactgcacc gttatcgctt acgccagcgt gttgccgaaa cttcgcgatc
    37141 gccagcagct tgccgcactg attcgtagtg gtgagctgga agtgcagggc gatattcagg
    37201 tggtgcaaaa cttcgttgcg ctggcagatc tggctgagtt cgaccctgcg gaactgctgg
    37261 ccccttatac cggtgatatc gccgctgaag gtatcagcaa agccctgcgc gggggcgcaa
    37321 agttcctgca tcacggcatt aagcgccagc aacgttatgt ggcggaagcc attactgaag
    37381 agtggcgtat ggcacccggt ccgcttgaag tggcctggtt tgcggaagag acggctgccg
    37441 tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa actggaggct aaatgacgcc
    37501 aggtgaagta cggcgcctat atttcatcat tcgcactttt ttaagctacg gacttgatga
    37561 actgatcccc aaaatgcgta tcaccctgcc gctacggcta tggcgatact cattattctg
    37621 gatgccaaat cggcataaag acaaaccttt aggtgagcga ctacgactgg ccctgcaaga
    37681 actgggacca gtatggatca agttcgggca gatgttatca acccgccgcg atctttttcc
    37741 gccgcatatt gccgatcagc tggcgttatt gcaggacaaa gtcgctccgt ttgatggcaa
    37801 gctggcgaag cagcagattg aagctgcaat gggcggcttg ccggtagaag cgtggtttga
    37861 cgattttgaa atcaagccgc tggcttctgc ttctatcgcc caggttcata ccgcgcgatt
    37921 gaaatcgaat ggtaaagagg tggtgattaa agttatccgc ccggatattt tgccggtcat
    37981 taaagcggat ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga
    38041 tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct tgattgatga
    38101 actgaatttg ctgcgggaat ctgccaacgc cattcagctt cgacgcaatt ttgaagacag
    38161 cccgatgctc tacatcccgg aagtttaccc tgactattgt agtgaaggga tgatggtgat
    38221 ggagcgtatt tacggcattc cggtgtctga tgttgcgacg ctggagaaaa acggcaccaa
    38281 catgaaattg ctggcggaac gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag
    38341 ctttttccat gctgatatgc accctggcaa catcttcgta agctatgaac acccggaaaa
    38401 cccgaaatat atcggcattg attgcgggat tgttggctcg ctaaacaaag aagataaacg
    38461 ctatctggcg gaaaacttta tcgccttctt taatcgcgac tatcgcaaag tggcagagct
    38521 acacgtcgat tctggttggg tgccaccaga taccaacgtt gaagagttcg aatttgccat
    38581 tcgtacggtc tgtgaaccta tctttgagaa accgctggcc gaaatttcgt ttggacatgt
    38641 actgttaaat ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt
    38701 gttactccag aaaaccctgc tctacgtcga aggggtagga cgccagcttt atccgcaact
    38761 cgatttatgg aaaacggcga agcctttcct ggagtcgtgg attaaagatc aggtcggtat
    38821 tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga
    38881 actgcctgaa ctggtttacg acagtttgcg ccagggcaag tatttacagc atagtgttga
    38941 taagattgcc cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc gttattttct
    39001 cggaattggc gctacgttag tattaagtgg cacattcttg ttggtcagcc gacctgaatg
    39061 ggggctgatg cccggctggt taatggcagg tggtctgatc gcctggtttg tcggttggcg
    39121 caaaacacgc tgattttttc atcgctcaag gcgggccgtg taacgtataa tgcggctttg
    39181 tttaatcatc atctaccaca gaggaacatg tatgggtggt atcagtattt ggcagttatt
    39241 gattattgcc gtcatcgttg tactgctttt tggcaccaaa aagctcggct ccatcggttc
    39301 cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg aaccaaagca
    39361 ggataaaacc agccaggatg ctgattttac tgcgaaaact atcgccgata agcaggcgga
    39421 tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc cacgataaag agcaggtgta
    39481 atctgtgttt gatatcggtt ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt
    39541 tctggggccg caacgactgc ctgtggcggt aaaaacggta gcgggctgga ttcgtgcgtt
    39601 gcgttcactg gcgacaacgg tgcagaacga actgacccag gagttaaaac tccaggagtt
    39661 tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa
    39721 agcgtcgatg gatgaattac gccaggctgc ggagtcgatg aagcgttcct acgttgcaaa
    39781 cgatcctgaa aaggcgagcg atgaagcgca caccatccat aacccggtgg tgaaagacaa
    39841 tgaaactgtg catgaaggcg taacgcctgc tgctgcacaa acgcaggcca gttcgccgga
    39901 acagaagcca gaaaccacgc cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa
    39961 aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt agaagatact
    40021 caaccgctta tcacgcatct gattgagctg cgtaagcgtc tgctgaactg cattatctcg
    40081 gtgatcgtga tattcctgtg tctggtctat ttcgccaatg acatctatca cctggtatcc
    40141 gcgccattga tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg
    40201 ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc agcgccggtg
    40261 attctctatc aggtgtgggc gtttatcgcc ccagcgctgt ataagcatga acgtcgcctg
    40321 gtggtgccgc tgctggtttc cagctctctg ctgttttata tcggcatggc gttcgcctac
    40381 tttgtggtct ttccgctggc atttggcttc cttgccaata ccgcgccgga aggggtacag
    40441 gtatccaccg acatcgcgag ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt
    40501 gtctcctttg aagtgccggt ggcaattgtg ctgctgtgct ggatggggat tacctcgcca
    40561 gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt cgggatgttg
    40621 ctgacgccgc cggatgtctt ctcgcaaacg ctgttggcga tcccgatgta ctgcctgttt
    40681 gaaatcggtg tcttcttctc acgcttttac gttggtaaag ggcgaaaccg ggaagaggaa
    40741 aacgacgctg aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg
    40801 gttgtcatat ggagtacagg atgtttgata tcggcgttaa tttgaccagt tcgcaatttg
    40861 cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat gggctactca
    40921 tcaccggtac caatctgcgt gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt
    40981 cctgttggtc aacggcgggc gtacatcctc acgacagcag ccagtggcaa gctgtgactg
    41041 aagaagcgat tattgagctg gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc
    41101 tcgactttaa ccgcaacttt tcgacgccgg aagagcagga acgcgctttt gttgcccagc
    41161 tacgcattgc cgcagaatta aacatgccgg tatttatgca ctgtcgcgat gcccacgagc
    41221 ggtttatgac attgctggag ccgtggctgg ataaactgcc tggtgcggtt cttcattgct
    41281 ttaccggcac acgcgaagag atgcaggcgt gcgtggcgtg tggaatttat atcggcatta
    41341 ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg ccgttgattc
    41401 cggcggagaa attgctgatc gaaactgatg cgccgtatct gctccctcgc gatctcacgc
    41461 caaagccatc atcccggcgc aacgcgccag cccatctgcc ccatattttg caacgtattg
    41521 cgcactggcg tggagaagat gccgcatggc tggctgccac cacggatgcc aatgtcaaaa
    41581 cactgtttgg gattgcgttt tagagtttgc ggaactcggt gttctttacg ctgtgcttaa
    41641 tttctttatt gataagatta agcaatagca tggagcgagc ctctccgtct ggctcggtga
    41701 aaatggcctg aaaaccttcg aatgcgcctt cagtaataat cactttatca ccaggatacg
    41761 gcgtcgatgg atcgacaata tctttcggtt tatacactga taactgatga atcaccgccg
    41821 atgggactat cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg cgggtcgcgt
    41881 tgatagtcgt ggtatgaatc acttccggat cgaactccac aaacaagtag ttggggaaca
    41941 atggctcact gactgcagta cgtttgccac gcacgatttt ttccagggtg atcatcggtg
    42001 ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc acgttgaagt tgcccgcgct
    42061 tgcagtacag taaataccag gattgcataa tgactcttat ccgtttaatc ggggcgcaag
    42121 gatagcaaaa gctttacgct aagttaatta tattccccgg tttgcgttat accgtcagag
    42181 ttcacgctaa tttaacaaat ttacagcatc gcaaagatga acgccgtata atgggcgcag
    42241 attaagaggc tacaatggac gccatgaaat ataacgattt acgcgacttc ttgacgctgc
    42301 ttgaacagca gggtgagcta aaacgtatca cgctcccggt ggatccgcac ctggaaatca
    42361 ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc gctgttgttc gaaaacccta
    42421 aaggctactc aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc gtggcgatgg
    42481 gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg taaattattg gcgtttctga
    42541 aagagccgga gccgccaaaa ggtttccgcg acctgtttga taaactgccg cagtttaagc
    42601 aagtattgaa catgccgaca aagcgactgc gtggtgcacc ctgccaacaa aaaatcgtct
    42661 ctggcgatga cgtcgatctc aatcgcattc ccattatgac ctgctggccg gaagatgccg
    42721 cgccgctgat tacctggggg ctcaccgtaa cgcgtggccc gcataaagag cggcagaatc
    42781 tgggcattta tcgccagcag ctaattggta aaaacaaact gattatgcgc tggctgtcgc
    42841 atcgcggcgg cgcgttggat tatcaggagt ggtgtgcggc gcatccgggc gaacgtttcc
    42901 cggtttctgt ggcgctgggt gccgatcctg ccacgattct cggtgcagtc acccccgttc
    42961 cggatacgct ttcagagtat gcgtttgccg gattgctgcg cggtaccaag accgaagtgg
    43021 tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc ggagattgtg ctggaagggt
    43081 atatcgaaca aggcgaaact gcgccggaag ggccgtatgg cgaccatacc ggttactata
    43141 acgaagtcga tagttttccg gtatttaccg tgacgcatat tacccagcgt gaagatgcga
    43201 tttatcattc cacctatacc gggcgtccgc cagatgaacc tgcggtactg ggtgtagcac
    43261 tgaacgaagt gttcgtgccg attctgcaaa aacagttccc ggaaattgtc gatttttatc
    43321 tgccgccgga aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa cagtacgccg
    43381 gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt atgtacacta
    43441 aatttgtgat cgtttgcgat gatgacgtca acgcccgcga ctggaacgat gtgatttggg
    43501 cgattaccac ccgtatggac ccggcgcggg atactgttct ggtagaaaat acgcctattg
    43561 attatctgga ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg ctggatgcca
    43621 cgaataaatg gccgggtgaa acccagcgtg aatggggacg tcccatcaaa aaagatccag
    43681 atgttgtcgc gcatattgac gccatttggg atgaactggc tatttttaac aacggtaaaa
    43741 gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg acagagaaag cgcatgacaa
    43801 ccttaagctg taaagtgacc tcggtagaag ctatcacgga taccgtatat cgtgtccgca
    43861 tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta tttgatggta gtgatggatg
    43921 agcgcgacaa acgtccgttc tcaatggctt cgacgccaga tgaaaaaggg tttatcgagc
    43981 tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc agtcatggac cgcatcctca
    44041 aagatcatca aatcgtggtc gatattcccc acggagaagc gtggctgcgc gatgatgaag
    44101 agcgtccgat gattttgatt gcgggcggca ccgggttctc ttatgcccgc tcgattttgc
    44161 tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc gggcgtgaag
    44221 agcagcatct gtatgatctc tgcgagcttg aggcgctttc gttgaagcat cctggtctac
    44281 aagtggtgcc ggtggttgaa caaccggaag cgggctggcg tgggcgtact ggcaccgtgt
    44341 taacggcggt attgcaggat catggtacgc tggcagagca tgatatctat attgccggac
    44401 gttttgagat ggcgaaaatt gcccgtgacc tgttttgcag cgagcgtaat gcgcgggaag
    44461 atcgcctgtt tggcgatgcg tttgcattta tctgagatat aaaaaacccg cccctgacag
    44521 gcgggaagaa cggcaactaa aactttatac agtggcatta ctctacgttg catccggcaa
    44581 acagtgcccg atgcaacgct gccgcgtctt atcgggccta caaaagttct gaaccgtggg
    44641 ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgttgca
    44701 atgccctgac ccagaccgat acacatcgtc gccagaccaa actggacgtc tttgtgttcc
    44761 atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg atgacccagc
    44821 gcgatcgcgc caccgttgag gttgatcttc tcgtcaatct gctccattag cccgagatct
    44881 ttaatacacg gcaggatctg cgcggcaaag gcttcgttca tctcgaatac gccgatatcg
    44941 ctggcagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg accataaccc
    45001 ataatagatg ggtcacaacc cacaaccgcc atcgaacgca cgcgggcgcg cggcttaaga
    45061 cctaattcac gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagc
    45121 gcagaagatg tacccgccgt taccgtaccg ctgacgggat caaacgccgg acgcagtgtg
    45181 gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcaat
    45241 acgccgtcgg catcgtgacc accggtcggg atgatttcat ttttaaatgc gcctgactgc
    45301 gtggcggccc acgcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg
    45361 ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg
    45421 acattacggc tcaggccggg gtgaaaatcg acgccgtgac tcattggcac atggcccata
    45481 tgctccacgc cgccaaccag acatgcctgc gcatcaccgg tcatgatcat tcgtgctgcg
    45541 tcatgcagtg cctgcatgga tgaaccacac aagcgattaa cggtaaccgc cgggacagag
    45601 tgcggcactt cagccaacag tgccgcgtta cgggcgatat taaaaccctg ctccagtgtt
    45661 tgctgcacac aaccccagta aatatcgtca agggccgccg cttccagtgc cgggttacgc
    45721 gccagcaggc tacgcattaa atgagcggag agatcttccg cacgcacgtt acgaaaagca
    45781 ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt
    45841 gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac cgggggatag
    45901 tacggttcgt tatgacgtgc tttattacgc agaccttccg gtacttcata cagcgggccg
    45961 aggtgctgat attgctgtgc catatcgagg tattttgcgc tgccgagggt gtccagccag
    46021 cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct
    46081 tctgccggag tggcgataat gccctcttcc agacagcgga ccacttcgtt gaccatcggg
    46141 atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggctg gctcactttt
    46201 gccagcaggt cgtcaacgac gacatcttct tctttcttcg gcttaccttt gctgtcttct
    46261 ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg
    46321 tcgatggcat cgcgataatc tttctgcatc cgctgcggga agcctgctgc catgacagcc
    46381 tgggcgtgat gcgcggtgtc aatgcccaca acatccagca gatatgccgg acccatcggc
    46441 cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc
    46501 aactggctga aaccggcgaa ataggggaac agtacacggt tgacgaagaa gccggggcag
    46561 tcgttaacca caatcggcgt tttgcccatc ttgctcgccc aggcgacgac tttcgcgatg
    46621 gtttcgtcgg agcttttctc gccgcgaata atttctacca acggcattcg gtggaccggg
    46681 ttaaagaagt gcatcccgca gaagttttcc ggacgttcca gcgcgttggc cagttcgctg
    46741 ataggaatgg ttgaagtgtt cgacgccagt acggtatccg ggcgtacttt ctgctcggtt
    46801 tctgccagca cggctttttt cactttcggg ttttcaacaa ccgcttctac cacaacatcc
    46861 acgcggtcaa acccggcgta gtcgagcgtt gggtgaattg tggagatcac gccagccagt
    46921 ttcagaccat cgatcttgcc gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc
    46981 atgccgaggg ttaacgactt gtcgttgata tctttcatga caaccggcac gcctttccac
    47041 gcagactggt aagcgatgcc gccgcccata atgcctgcgc ccagcacagc ggcctgtttc
    47101 ggggtttcaa catctttggt gagtttcttc gctttacctt ttacatattg atcgttaagg
    47161 aaaatgccga ccagtgcgcg ggcttcgttg gtatgcgcca gcgggacaaa acttttgttt
    47221 tccaggttta aggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgcg
    47281 gtgatggggg ccggataatg tttccccgcc gtttgtgcga ccatcccttt agcgatggtg
    47341 aagctcatgg tggcttcaat cttgctcagt tttagcggtt ccagcttcgg ctgacgtttc
    47401 gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa tcgccatcgc cccttcaatc
    47461 agtttttctg ctttgactac accatccacc agaccgattt tcagcgcctg atccgcgccg
    47521 acatctttac cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata
    47581 cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc
    47641 agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc accgcccagc
    47701 gcatagccgt tgacggcagc aatggtcggc accgggagat cttccaggcg attaaacacg
    47761 ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa
    47821 aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgcag cagcagccct
    47881 tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca
    47941 gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc
    48001 agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag
    48061 gtgatctggt cgtaccagat gcatcgaagt gtgtattttg tgttaaaaat atgcaaataa
    48121 aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat
    48181 gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg
    48241 cctcactcta taaaaatcat atcgctacct tacaagaacg gactcgcgat gcgctggcgc
    48301 gcttcaagct ggatgcgtta cttattcact ccggcgaact gttcaacgtt tttctcgacg
    48361 atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg
    48421 tgccaaactg ctggctgctg gtggatggcg tgaacaagcc gaaactgtgg ttttatctgc
    48481 cggttgatta ctggcacaac gtcgaaccgc taccgacctc cttctggact gaagatgtag
    48541 aagtgatcgc gctgccgaaa gccgatggca ttggtagcct gctgcctgct gcgcgcggca
    48601 atatcggtta tatcggtccg gtgccggaac gtgcgctgca actgggtatt gaggccagca
    48661 acatcaaccc gaaaggggtg atcgactacc tgcattacta ccgctccttc aaaaccgagt
    48721 acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caatggtcat cgtgcggcag
    48781 aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa cattgcctat ctgaccgcga
    48841 ccggtcatcg tgataccgac gtaccttata gcaacattgt ggcgcttaac gaacacgctg
    48901 cggtgttgca ttacaccaaa ctggaccacc aggcaccgga agagatgcgc agcttcctgc
    48961 tggatgccgg ggccgaatat aacggctatg cggctgatct gacccgtacc tggtcggcaa
    49021 aaagcgacaa cgactacgca cagctggtga aagacgtaaa tgatgagcaa ctggcgctga
    49081 tcgccaccat gaaagcaggc gtcagctatg tggattacca tatccagttc catcagcgca
    49141 tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg
    49201 aaaacgatct caccggaccg tttatgccgc acggtatcgg ccatccgctg ggcctgcagg
    49261 tgcatgacgt agccggtttt atgcaggatg atagcggtac gcacctcgcg gcaccggcaa
    49321 aatatccgta cctgcgctgc acccgtattc tccagccggg catggtgtta accatcgaac
    49381 cgggtatcta cttcatcgaa tcgctgctgg caccgtggcg tgaagggcag ttcagcaaac
    49441 acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca
    49501 acgtggtgat tcacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat
    49561 ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaagagccg
    49621 cttcattacg ctgttggcgc ataccgatgg cgttgaggcg gcaaaagcgt ttgttgaatc
    49681 ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc
    49741 ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa cggcaggtaa
    49801 accgatgctc gcccaactaa tgggcagcgg cgtcggggaa attaccgctg tggtggtgcg
    49861 ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt
    49921 gaatcaggcg ctgcgccagc taacgaccca acgcaagaca ccgttaaccg aatatacttt
    49981 gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa
    50041 aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa
    50101 agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc
    50161 gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt
    50221 cgcgccatta cccgaatcgt tggactactg gttatcttat tttcagggac catgattatc
    50281 cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt
    50341 tttgtcgccc tcgccattgg ctctatgctt tggtggccga accgcaaaga gaagggtgaa
    50401 ctgaaatccc gtgaggggtt tctgatagtg gtgctgttct ggaccgtgct gggtagcgtc
    50461 ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt
    50521 gaatctttct ctggcctgac caccacgggg gccactacgc tggtggggct ggattcgctc
    50581 cctcacgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc
    50641 gtgttggcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca
    50701 gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa
    50761 accctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgct
    50821 ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc
    50881 tcgacacatg atgccagtat cggttatttc gacagcccga ctattaacac tatcattgct
    50941 atcttcctgc tgatctctgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt
    51001 agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagttttca
    51061 ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg
    51121 acaattaacc aggcgttttt ccaggtggtg tcgatggcga caaccgccgg gtttacaact
    51181 gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc
    51241 ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gttgctgttt
    51301 aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag
    51361 ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc
    51421 tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt
    51481 tctgcctttg cctcggttgt tgcaacattg aataacctgg ggccagggct tggcgtggtt
    51541 gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg
    51601 tttggtcgtc tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa
    51661 tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg
    51721 agattgcctc ctacctggct tcggaactga aagaactggg gatccaggcg gatgtcgcca
    51781 atgtgcaccg cattgaagaa ccacaatggg aaaactatga ccgtgtggtt attggtgctt
    51841 ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaag catgcgacgc
    51901 gactgaattc gatgccgagc gccttttatt ccgtaaatct ggtggcgcgt aaaccggaga
    51961 agcgtactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc
    52021 ccgatcgctg tgccgtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgacc
    52081 gttttatgat caagctgatt atgaagatgt caggcggtga aacggatacg cgcaaagaag
    52141 ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg
    52201 acaaaccgac gctgaaataa gcataaagaa
//
LOCUS       DAOFSV010000016        51074 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.016, whole genome shotgun sequence.
ACCESSION   DAOFSV010000016 DAOFSV010000000
VERSION     DAOFSV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.016"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..555)
                     /locus_tag="QBH27_001047"
     CDS             complement(<1..555)
                     /locus_tag="QBH27_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDQ7013387.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVDCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAI"
     gene            748..1251
                     /locus_tag="QBH27_001048"
     CDS             748..1251
                     /locus_tag="QBH27_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ7013388.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1297..1713)
                     /gene="rraB"
                     /locus_tag="QBH27_001049"
     CDS             complement(1297..1713)
                     /gene="rraB"
                     /locus_tag="QBH27_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDQ7013389.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1875..2879
                     /gene="argF"
                     /locus_tag="QBH27_001050"
     CDS             1875..2879
                     /gene="argF"
                     /locus_tag="QBH27_001050"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDQ7013390.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNTLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNNKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2936..4532)
                     /locus_tag="QBH27_001051"
                     /pseudo
     CDS             complement(2936..4532)
                     /locus_tag="QBH27_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(4655..5107)
                     /gene="tabA"
                     /locus_tag="QBH27_001052"
     CDS             complement(4655..5107)
                     /gene="tabA"
                     /locus_tag="QBH27_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDQ7013391.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYKARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLVDKDIAF
                     LPEVVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(5252..5845)
                     /locus_tag="QBH27_001053"
     CDS             complement(5252..5845)
                     /locus_tag="QBH27_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ7013392.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            5916..6629
                     /gene="bdcA"
                     /locus_tag="QBH27_001054"
     CDS             5916..6629
                     /gene="bdcA"
                     /locus_tag="QBH27_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7013393.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            6760..7155
                     /locus_tag="QBH27_001055"
     CDS             6760..7155
                     /locus_tag="QBH27_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDQ7013394.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            7436..7570
                     /gene="pyrL"
                     /locus_tag="QBH27_001056"
     CDS             7436..7570
                     /gene="pyrL"
                     /locus_tag="QBH27_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDQ7013395.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            7574..8509
                     /gene="pyrB"
                     /locus_tag="QBH27_001057"
     CDS             7574..8509
                     /gene="pyrB"
                     /locus_tag="QBH27_001057"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDQ7013396.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            8522..8983
                     /gene="pyrI"
                     /locus_tag="QBH27_001058"
     CDS             8522..8983
                     /gene="pyrI"
                     /locus_tag="QBH27_001058"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDQ7013397.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            9056..9442
                     /gene="ridA"
                     /locus_tag="QBH27_001059"
     CDS             9056..9442
                     /gene="ridA"
                     /locus_tag="QBH27_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDQ7013398.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(9648..12344)
                     /gene="mgtA"
                     /locus_tag="QBH27_001060"
     CDS             complement(9648..12344)
                     /gene="mgtA"
                     /locus_tag="QBH27_001060"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDQ7013399.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(12485..12538)
                     /gene="mgtL"
                     /locus_tag="QBH27_001061"
     CDS             complement(12485..12538)
                     /gene="mgtL"
                     /locus_tag="QBH27_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDQ7013400.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12723..13670
                     /gene="treR"
                     /locus_tag="QBH27_001062"
     CDS             12723..13670
                     /gene="treR"
                     /locus_tag="QBH27_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDQ7013401.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            13789..15210
                     /gene="treB"
                     /locus_tag="QBH27_001063"
     CDS             13789..15210
                     /gene="treB"
                     /locus_tag="QBH27_001063"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDQ7013402.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGNFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            15260..16915
                     /gene="treC"
                     /locus_tag="QBH27_001064"
     CDS             15260..16915
                     /gene="treC"
                     /locus_tag="QBH27_001064"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDQ7013403.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            17309..19447
                     /gene="nrdD"
                     /locus_tag="QBH27_001065"
     CDS             17309..19447
                     /gene="nrdD"
                     /locus_tag="QBH27_001065"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDQ7013404.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            19606..20070
                     /gene="nrdG"
                     /locus_tag="QBH27_001066"
     CDS             19606..20070
                     /gene="nrdG"
                     /locus_tag="QBH27_001066"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDQ7013405.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(20115..20501)
                     /gene="cybC"
                     /locus_tag="QBH27_001067"
     CDS             complement(20115..20501)
                     /gene="cybC"
                     /locus_tag="QBH27_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDQ7013406.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(20684..22036)
                     /gene="pmbA"
                     /locus_tag="QBH27_001068"
     CDS             complement(20684..22036)
                     /gene="pmbA"
                     /locus_tag="QBH27_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDQ7013407.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            22130..22681
                     /locus_tag="QBH27_001069"
     CDS             22130..22681
                     /locus_tag="QBH27_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDQ7013408.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(22832..24205)
                     /gene="mpl"
                     /locus_tag="QBH27_001070"
     CDS             complement(22832..24205)
                     /gene="mpl"
                     /locus_tag="QBH27_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDQ7013409.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            24381..25379
                     /gene="fbp"
                     /locus_tag="QBH27_001071"
     CDS             24381..25379
                     /gene="fbp"
                     /locus_tag="QBH27_001071"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDQ7013410.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(25412..26407)
                     /gene="yjfF"
                     /locus_tag="QBH27_001072"
     CDS             complement(25412..26407)
                     /gene="yjfF"
                     /locus_tag="QBH27_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDQ7013411.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(26394..27416)
                     /locus_tag="QBH27_001073"
     CDS             complement(26394..27416)
                     /locus_tag="QBH27_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013412.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(27430..28932)
                     /locus_tag="QBH27_001074"
     CDS             complement(27430..28932)
                     /locus_tag="QBH27_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013413.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(29072..30028)
                     /gene="ytfQ"
                     /locus_tag="QBH27_001075"
     CDS             complement(29072..30028)
                     /gene="ytfQ"
                     /locus_tag="QBH27_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDQ7013414.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            30338..30868
                     /gene="ppa"
                     /locus_tag="QBH27_001076"
     CDS             30338..30868
                     /gene="ppa"
                     /locus_tag="QBH27_001076"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDQ7013415.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(30948..31298)
                     /gene="chpB"
                     /locus_tag="QBH27_001077"
     CDS             complement(30948..31298)
                     /gene="chpB"
                     /locus_tag="QBH27_001077"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDQ7013416.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(31292..31543)
                     /gene="chpS"
                     /locus_tag="QBH27_001078"
     CDS             complement(31292..31543)
                     /gene="chpS"
                     /locus_tag="QBH27_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDQ7013417.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(31755..32096)
                     /locus_tag="QBH27_001079"
     CDS             complement(31755..32096)
                     /locus_tag="QBH27_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDQ7013418.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(32099..35878)
                     /gene="tamB"
                     /locus_tag="QBH27_001080"
     CDS             complement(32099..35878)
                     /gene="tamB"
                     /locus_tag="QBH27_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDQ7013419.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(35875..37608)
                     /gene="tamA"
                     /locus_tag="QBH27_001081"
     CDS             complement(35875..37608)
                     /gene="tamA"
                     /locus_tag="QBH27_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDQ7013420.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            37814..38452
                     /gene="msrA"
                     /locus_tag="QBH27_001082"
     CDS             37814..38452
                     /gene="msrA"
                     /locus_tag="QBH27_001082"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDQ7013421.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            38775..40118
                     /locus_tag="QBH27_001083"
     CDS             38775..40118
                     /locus_tag="QBH27_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDQ7013422.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(40180..40386)
                     /locus_tag="QBH27_001084"
     CDS             complement(40180..40386)
                     /locus_tag="QBH27_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDQ7013423.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            40711..41268
                     /locus_tag="QBH27_001085"
     CDS             40711..41268
                     /locus_tag="QBH27_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDQ7013424.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(41258..41998)
                     /gene="cysQ"
                     /locus_tag="QBH27_001086"
     CDS             complement(41258..41998)
                     /gene="cysQ"
                     /locus_tag="QBH27_001086"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDQ7013425.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            42188..44131
                     /gene="cpdB"
                     /locus_tag="QBH27_001087"
     CDS             42188..44131
                     /gene="cpdB"
                     /locus_tag="QBH27_001087"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDQ7013426.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(44260..44640)
                     /locus_tag="QBH27_001088"
     CDS             complement(44260..44640)
                     /locus_tag="QBH27_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDQ7013427.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            44729..45589
                     /gene="qorB"
                     /locus_tag="QBH27_001089"
     CDS             44729..45589
                     /gene="qorB"
                     /locus_tag="QBH27_001089"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDQ7013428.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETDKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            45697..46662
                     /locus_tag="QBH27_001090"
     CDS             45697..46662
                     /locus_tag="QBH27_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ7013429.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            46770..47432
                     /gene="ytfE"
                     /locus_tag="QBH27_001091"
     CDS             46770..47432
                     /gene="ytfE"
                     /locus_tag="QBH27_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDQ7013430.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(47477..48889)
                     /gene="cycA"
                     /locus_tag="QBH27_001092"
                     /gene_synonym="dagA"
     CDS             complement(47477..48889)
                     /gene="cycA"
                     /locus_tag="QBH27_001092"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDQ7013431.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(49198..49818)
                     /gene="fklB"
                     /locus_tag="QBH27_001093"
     CDS             complement(49198..49818)
                     /gene="fklB"
                     /locus_tag="QBH27_001093"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ7013432.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            50037..50675
                     /gene="ytfB"
                     /locus_tag="QBH27_001094"
     CDS             50037..50675
                     /gene="ytfB"
                     /locus_tag="QBH27_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDQ7013433.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     ESQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(50659..>51074)
                     /locus_tag="QBH27_001095"
     CDS             complement(50659..>51074)
                     /locus_tag="QBH27_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ7013434.1"
                     /translation="SDLPPQKAIEKIIDFTWDYYISNPWFLKIVHSENQSKGVHYAKS
                     QRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVY
                     HISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
BASE COUNT        12698 a        13463 c        12579 g        12334 t
ORIGIN      
        1 tattgccatc agaaccacag atataataac actacttgaa ctattggcat gtagcatctt
       61 tgtcgagata aagaaaacag tccccatccc aatggccatt aaaatcggca agaaaaaggt
      121 tacccaccag aaccctttca aagagcaatt aaaactaaat cttacgccgt tgagactagt
      181 catcaaggcc tgataacgca gtccttttgc tgccataaat ataagcaaaa ccaacagtga
      241 caaagtcaaa acacagtcaa caagcggcat atctgctgac actgtcatta agattgcgaa
      301 atagaaaaaa acaaaaacaa gacaactaac aaaaacattc ccaccggtaa tcccataaga
      361 aaatcgttgt ccgttaactt ccatattagc atagagataa cgcttacatt tcattaatgc
      421 ccatggtaaa tagataccta gcgttataat cgttaacaac acattcacca cacaaataag
      481 aaaatatctc tcacctgtac catgaaaaac aaacgaatgg gacggaacat ccatttcatt
      541 aataacttga gccataaaaa ttccctgaat taaaaaattc attgatataa tttatagata
      601 cagacagtaa aatgttctgc cacgttacaa tactcttatc agtaataggc tggcaaaaac
      661 tttattgctt tataagacgc ttgcgcacgt aagaagagag tggtattaaa caaatgtata
      721 attactttat ttttattggg ttaaactatg aataacattg cgccgcaatc acctgtaatg
      781 cgtcgcctga cgttgcagga taatcctgct atcgcccgcg tcattcgtca ggtatccgcc
      841 gaatacggtc ttaccgctga taaaggctac accgtcgccg atccgaatct tgacgagctg
      901 tatcaagtgt atagtcagcc tggtcatgca tattgggtgg ttgagtacga gggtgaagtg
      961 gtcggcggcg gtgggattgc gccattagca ggtagtgaat cggacatttg cgaactgcaa
     1021 aagatgtatt ttcttccggc tattcgcggt aaagggctgg cgaaaaaact ggccttaatg
     1081 gcgatggagc aagcacgaga gatgggcttc aaacgctgct atctggaaac gaccgctttt
     1141 ttaaaggaag ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcgctt
     1201 ggctgcacgg gccatgtcga ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc
     1261 cgctagtgtg tgctatcggg cattttaatc aattaattag tggcgaacac cgtcatcgtc
     1321 ttcatcgaca aaatcttcat cgtcgccgtc ttcgccattg ggatcttcaa agtaagtgcc
     1381 ccaaccgtcg tactcaacgt caaatttctc cgccagcgtc atcagttgtt caacctgggc
     1441 atcgatcaga tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc
     1501 accatcttca acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc
     1561 aactgctgct ttttccaggg tttccagatc gtctgcggaa agatggtgtt cgatggtgta
     1621 cagcgcgtcc ggatcgctgc catcttccag taattcttca ataatcaaac gtgtttcttc
     1681 acgctgttct tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga
     1741 tactcttatt gtcacacacc actgacattg cctccacctt tgtgacaaag atttatgctt
     1801 tagacttgca aatgaataat catccatata aattgaattt taattcattg aggcattagc
     1861 cacaggaggg atctatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca
     1921 cgccagctga actcaacacc ttgttgcagt tagccgcgaa gctgaaagcc gataagaaaa
     1981 gcggtaaaga agaagctaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact
     2041 cgactcgtac ccgatgctct ttcgaagttg ccgcatatga ccagggtgct cgtgttactt
     2101 atctcggccc aagcggcagc cagattggtc ataaagagtc gattaaagac actgcccgcg
     2161 tgcttggtcg catgtatgac ggtattcagt atcgcggcta tggtcaggag attgtcgaaa
     2221 cactggcgga atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca
     2281 cgcagctgct ggcggatctc ctcaccatgc aggagcattt gcccggtaaa acattcaacg
     2341 aaatgacgct ggtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag
     2401 ctgcggcgct taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag
     2461 ctgcgctggt tacggaatgc cgcgctctgg cacagcaaaa tggcgggaat attacgctga
     2521 cggaagatgt agccaaagga gtagaaggtg ctgactttat ctataccgat gtgtgggtgt
     2581 cgatggggga agcaaaagag aaatgggcgg aacgaattgc attgctgcgt gattatcagg
     2641 tgaataacaa gatgatgcag ttgaccggta acccggaggt caaattcctc cactgcctgc
     2701 ccgcatttca tgacgaccaa acgacgcttg gcaagaaaat ggcggaagag tttggcctac
     2761 atggcggaat ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtatttgatc
     2821 aggcggaaaa ccgcatgcat accatcaaag cggtgatggt cgcgacgctc agtaaataat
     2881 tgatatcgct ataaagatgc cgggaaatac aaaactatcc cggcatcaca ttattttatg
     2941 cagtgatagg tctattcttc cacccaattt tttctattgc agaacgtaaa tcttgcggtg
     3001 gatttaattt ttctaactgt ttttcattaa tataatcata tatattgatt ttcccatccc
     3061 gcagggtttc catctttctt gttctaattt ttaattcatt atcgatcttt tcttcaaaaa
     3121 tcttaacgac ataacccgga agtttattta caatttcttt tgttttttct tcatcaccat
     3181 aaacatatcc taactcatta agatacatct gttctccctc ataactctta agtaattgat
     3241 agaagtacca cttagcaaaa tcaggttgct tcatgaaaaa atccttcaaa aaaaatatca
     3301 ttcactaaaa tactgagagg cactttatca aaaaccaatt taacatcatc aaatagcctt
     3361 agtattaagc gcgatttttc ttgattactt aaattaaaat cattgacata tacaaccaac
     3421 ttctcccaaa tcaattcctg taactcaggg ctgtttttat agggttcttt attgaggttg
     3481 aaaaataatg tctcagctat tttagactga tcgcagttaa aatcaatatg atcgatcacg
     3541 gagcgggcat gctttatctt tgtagtatca tcagaatatt gcatactttg attaaaacac
     3601 cactccagaa tttttacatc attaatatat ggcaatttta aaaaagtctt caacatcgaa
     3661 tcgatgacct tttcgagatt aacttttgca tcatctaagg aatcaataag aatattagac
     3721 aggttaattt tgatgttatt aatttcatca ttaccttctg taccactagc aattctggta
     3781 tcatgtaacg ttttcagatc atttatttct tcaagtatac caccttcgcc tagtaagtat
     3841 ggaatcgtgt catcacatac atgaggcatt aatagcagat gttctttctt tattttttct
     3901 aatgtactgg ctaatggctt ttctaataac tcgttataat caattgcgtc cttccagttc
     3961 aactgatgat tatttaatag aagcgccata ttcttgttag cttcacgttg gtaacacgcc
     4021 agaaccttgt tagtaatcag gtgtaatgaa ctatcatctt ttctttcaaa agacttacct
     4081 tcataacgaa aactcaaaaa atcagattgt ggaaatataa tggctgtatg tccttgacga
     4141 gtccccatag tagtggtagt agaaagtgaa atttctataa tcaccttaga tgaatccgca
     4201 cgctcacctt caggattatt cataacaaaa ttcattattt cactgccatc attatttttg
     4261 atataagatg aggactcaat atgagccatc atatttctac ccgcttcgtt ggagcatttg
     4321 ctaaaatcca cataacccag ggtaaatatt tttgccaaaa actccaaaaa tgctccagga
     4381 gatagaatat tccttctgct atcctgcccc tcggcattac gagcaaaatc attcattccc
     4441 acacaccagg tatttaagac ttcatttttt ggtttaacat tgtcggctga tgccatgtgt
     4501 tgataacaat tcaaatctga tatttttgcc atgacgaact ccttgagtta ataagaacgt
     4561 ctaagcatta caaattttac tcacaaaaaa atatcaaaaa tcgctctttt cacaaactcg
     4621 ccgtaaacct ggagttattt acggcgaaaa gtcatcacgc catcagcatc ttcactactg
     4681 ctttgcgaac ctgggctggt gcacccactg cgcacagcgg tttatgcact tcccccggat
     4741 aaaacacaac aaaatcacct tcattcagga taactgtttt ctcatcaacg acttccggca
     4801 aaaatgcgat gtctttatct actaaccagt cggtctccgg cgtacctgca ggttgtgtgc
     4861 tgaaggtcat gccttcctga ccttttaaca caatctgaat gtcgagataa cgggcatggt
     4921 actccgcacg gcgagctttg tacggctcgg tcatatcttc cgagataaga taaaacagac
     4981 gattgccttc gatatcgtgc ttaccctttg gcgtttctgc cgtaacgtgt gctttgatat
     5041 gctcaatcgc ctggcgtaac tcctggggta gccatggctg aagattatga atatttccga
     5101 tgatcatctt ttccactcct taattaaaac aatgtttcat ttccaccgtt atacgaaaag
     5161 atgatcaatt ccgccagcct taacgcgtga attattgtct tatcacaact tatcggcagg
     5221 cgcgttcccg cctttcggat caaggtcagc atcactcctt gagaatagtt tttatcgctt
     5281 cccccgccag tgcagctgtt gcacagagtt gttcgaggga gtgcccctct cgtgccttcg
     5341 cagaaagccc tgacatcacg gtactcataa aatcagtcac acattgtgcg ctttgtggat
     5401 gccgcctggc gatatagtca taaatggtcg tttctgcggc gtgataatat tgaacggcaa
     5461 tatcacgcgc ttgtggatca tgactatgaa tgccttcaag aaccatacag ccagcgcagc
     5521 cgccgttttg gctatatctg cgcgccgctt cttttaatac ctcagccagg cactccccta
     5581 ccggacgatc atcacgaaga atatcggcaa gcggaatagc ttccgtaccg acatattcat
     5641 tgagtacacg gctaaataac ccagccttac tgccaaaagc cgcgtagaga ctcggggggt
     5701 taataccaag ataatcagta acttcagcaa cactgacagc atcgaaacct ttttggtgaa
     5761 aaagcacttt tgccgcagag actgcctgct caggagcgaa acgtctggga cgacctggaa
     5821 cgcgagattg ttttttagtg accattacaa aacttgttga cagaaagtta aaacagtttt
     5881 gtaatgcatg ttacataata aatcaaggag tccttatggg cgcttttaca ggtaagacag
     5941 ttctcatcct cggtggtagc cgtggaatag gtgccgctat cgtacgtcgt ttcgtcaccg
     6001 atggagccaa tgtacgattc acctatgcgg ggtcgaaaga tgccgctgaa cgcctggcac
     6061 atgagactgg agcgacagca gtattcacag atagtgctga cagagacgct gttattgatg
     6121 tcgttcgtaa gagcggcgca ttggatatcc tggtggtaaa tgcaggtatt ggcgtctttg
     6181 gcgatgccct ggaattaaat gccgacgata ttgatcgcct tttcaaaatc aatattcatg
     6241 ccccttacca tgcctctgtt gaagccgccc ggcagatgcc cgaaggcggg cgcatcttaa
     6301 tcatcggctc cgtgaatggc gatcgtatgc ctgttgcagg catggctgct tatgccgcca
     6361 gtaaatctgc cctgcaaggc atggcgcgcg ggctggcccg tgattttgga ccgcgtggga
     6421 tcaccatcaa cgtcgtccag ccagggccaa tagataccga cgctaatccc gccaacgggc
     6481 caatgcgcga tatgttgcat agttttatgg ctatcaaaag acatgggcaa ccggaagagg
     6541 tcgctggaat ggtcgcatgg ttagcagggc cagaagccag ttttgttacc ggcgcgatgc
     6601 ataccattga tggcgcgttt ggggcataac cgactacgct caattaagcc cagccattat
     6661 ccatgatgtc tgggttttgt ttactcacgt cgttccctaa aagcggctcc cggtaaatat
     6721 aaatcttctg ttcatgtcgt aaatcttgag gataaccaaa tggtagaaag aaccgctgtt
     6781 ttccctgctg gccgacattc actgtatgct gaacatcgtt attcggcggc tattcgttcc
     6841 ggcgatttgc tgtttgtttc cgggcaagta ggaagtcgag aggacggaac accagaaccc
     6901 gattttcagc aacaagtcag actggcattt gataatttgc atgcgaccct ggcagctgcg
     6961 ggatgcactt ttgacgatat cattgatgtt acgagctttc ataccgatcc ggaaaaacaa
     7021 tttgaagaca tcatgacggt caaaaatgaa atatttagcg cccctcctta tccaaactgg
     7081 acggcggtgg gcgttacctg gctggcaggc tttgattttg aaattaaagt gatagcgcgc
     7141 atccctgagc tgtaagcaat agtgttagcc gttcgttttc acactccgcc gtataagtcg
     7201 gatgaatgga ataaaatgca tatctgattg cgtgaaagtg aaaaagaaaa aagcaggaaa
     7261 tgtctgcaat tattgatacc gaacgacagt tctcttccag aatcaaagca aataaaaatg
     7321 catacatttt gacttttaat tcaaataaac cgtttgcgct gacaaaatat tgcatcaaat
     7381 gcttgcaccg cttctggcta tgagtataat gccggacaat ttgccgggag gatgtatggt
     7441 tcagtgtgtt cgacattttg tcttaccgcg tctgaaaaaa gacgctggcc tgccgttttt
     7501 cttcccgttg atcacccatt cccagcccct caatcgaggg gctttttttt gcccaggcgt
     7561 caggagataa aagatggcta atccgctata tcagaaacat atcatttcca taaacgacct
     7621 tagtcgcgat gaccttaatc tggtgctggc gacagcggcg aaactgaaag caaacccgca
     7681 accagagctg ttgaagcaca aagtcattgc cagctgtttc ttcgaagcct ctacccgtac
     7741 ccgcctctct ttcgaaacat ctatgcaccg cctgggtgcc agcgtggtgg gcttctccga
     7801 cagcgccaat acatcactgg gtaaaaaggg cgaaacgctg gccgatacca tttcggttat
     7861 cagcacttac gtcgatgcga tagtgatgcg tcatccgcag gaaggtgcgg cgcgcctggc
     7921 caccgagttt tccggcaatg taccggtact gaatgccggt gatggctcca accaacatcc
     7981 gacgcaaacc ttgctggact tattcactat tcaggaaacc caggggcgtc tggacaatct
     8041 ccacgtcgca atggttggtg acctgaaata tggccgcacc gttcactccc tgactcaggc
     8101 gttagcgaag ttcgacggca accgttttta cttcatcgcg ccggacgcgc tggcaatgcc
     8161 gcaatacatt ctggatatgc tcgatgaaaa agggatcgca tggagtctgc acagctctat
     8221 tgaagaagtg atggcggaag tagacatcct gtacatgacc cgcgtgcaaa aagagcgtct
     8281 ggacccgtcc gagtacgcca acgtgaaagc gcagtttgtt cttcgcgcca gcgatctcca
     8341 caacgccaaa gccaatatga aagtgctgca tccgctgccg cgtgttgatg agattgccac
     8401 cgatgtggat aaaacgccac acgcctggta cttccagcag gcaggcaacg ggattttcgc
     8461 tcgccaggcg ttactggcac tggttctgaa tcgcgatctg gtactgtaag gggaaataga
     8521 gatgacacac gataataaat tgcaggttga agctattaaa cgcggcacgg taattgacca
     8581 tatccccgcc cagatcggtt ttaagctgtt gagtctgttc aagctgaccg aaacggatca
     8641 gcgcatcacc atcggtctga acttgccttc tggcgagatg ggccgcaaag atctgatcaa
     8701 aatcgaaaat acctttttga gtgaagatca ggtagatcaa ctggcattgt atgcgccgca
     8761 agccacggtt aaccgtatcg acaactatga agtggtgggt aaatcgcgcc ccagtctgcc
     8821 agagcgcatc gacaatgtgc tggtctgccc gaacagcaac tgtatcagcc atgccgaacc
     8881 ggtttcttcc agctttgccg tgcgaaaacg cgccaatgac atcgcgctca aatgcaaata
     8941 ctgtgaaaaa gagttttccc ataatgtggt gctggccaat taattgcggt tggtaataaa
     9001 agtctggctc cctataatga gccagacttt ttaccgctgt aataaaggag aaatcatgag
     9061 caaaactatc gcgacggaaa atgcaccggc agctatcggt ccttacgtac agggcgttga
     9121 tctgggcaat atgatcatca cctccggtca gatcccggta aatccgaaaa cgggtgaagt
     9181 accggcagac gtcgctgcac aggcacgtca gtcgctggat aacgtaaaag cgatcgtcga
     9241 agccgctggc ctgaaagtgg gcgacatcgt taaaaccacc gtgtttgtga aagatctgaa
     9301 cgacttcgct accgttaatg ctacctacga agccttcttc accgaacaca acgccacctt
     9361 cccggcacgt tcttgcgttg aagtcgcccg tctgccgaaa gacgtgaaga ttgagatcga
     9421 agcgatcgct gttcgtcgct aatcttgatg gaaatccggg ctatcatgcc cggattaagt
     9481 ctgatgacaa acgcaaaatc gcctgatgcg ctacgcttat caggcctacg tgattcctgc
     9541 aatttattga atttgttggc cggataaggc atttacgccg catccggcat gaacaaagct
     9601 tactttgtct gcaatctgaa tcggggctat cgtgcccagt ttattcttta ttgccagccg
     9661 taacgacggc tatagaaccc tttcaccaac tgggttaatg tcatataccc tgccagaatc
     9721 gcaaccagcc acgggaaata gcttaacggc agcgcctgta attgcagata actggccagc
     9781 ggtgaaaacg gcaatgcgat cccgacaatc atcacgatca cggtcatgat cattaacggc
     9841 cacgatgcac agctctgaat aaacggcaca cggcgggtgc ggatcatatg cacaatcagc
     9901 gtttgcgaca gtaagcccac cacaaaccat cccgactgga acagcgtttg cgtttccggc
     9961 gtgttggcat ggaataccca ccacatcagg caaaacgtca gaatatcgaa gatcgagctg
    10021 atcggtccga agaagatcat aaagcgcccc agatccgccg ggttccaacg ctgcggcttt
    10081 tgaatttgct cgtcgtcgac gttatcaaac gggatcgcca cctgtgacac atcatacagc
    10141 aggttctgaa taagcaagtg taacggcaac atcggcagga agggcaagaa agcactcgcc
    10201 accagcacgc tgaacacatt accgaagttg gagctcgccg tcattttgat gtatttcagc
    10261 atgttggcga aagtgcgacg tccttcaata accccctctt ccagcaccat caggcttttt
    10321 tccagcagga tgatatcagc cgcttcacgg gcaatatcta ccgcgccgtc cacagaaatg
    10381 ccgatatccg cagcgcgtaa ggcgggcgca tcattaatac catcgcccat aaagccaacc
    10441 acatgccctt cgcgcttcag taaggtcaca atgcgttctt tatgcatcgg cgtcagacga
    10501 gcaaacagcg tggtacgctg tgcgagattt gccagttcgt cgtcagatag cgtttcaata
    10561 tcactaccaa tgaccacctc tcccgcgtcc agccccactt catggcacac tttcgctgca
    10621 actaactcac tgtcgccagt aaggattttt acggtaattc cactcgcttt taatgccttc
    10681 agtgccggag cagttgtctc tttcggcgga tcaaggaaag caatatatcc ttcgaggatc
    10741 aggtcggatt catccgcccg ctggtaatct ccttcacgcg ctggcaggta tttcgtcgcc
    10801 accgcaacca cgcgcagccc ctgacgattc agcgtatcag taacccgctt aatcttacgc
    10861 agcatgatgt catcgagcgg cacaatctcg ccattgtgac gcacctgcga acacacattg
    10921 aggatttcct gcaatgcacc tttgcaaacc agctgatggt gctcggtatt ttctgccact
    10981 accaccgaca tccggcgacg ctcgaaatca aacggaatct catcgatttt ttgccaacga
    11041 ctggccagcg agcgcgctga ctcttcatcc gtaccttcga gcaccgctgt atcgagcagg
    11101 tttttaagtc cggtctgata atgactgttc aaccacgcgc tatgcagcac gcgttcgctg
    11161 gttttaccgg agatatcggt atgattctcc agcacaattt tgtcctgtgt cagggtgcca
    11221 gttttatcag tgcacagaat atccattgcg ccaaagttct gaatagcatc cagatgtttg
    11281 acgatcactt tctgtttcga cagctttact gcgccgcgcg ccagcgtcga ggtaacaatc
    11341 atcggcaaca tttccggcgt taagcctacc gcgaccgaaa gcgcaaacag tgccgcttcc
    11401 caccagtcgc ctttggtgta accattgatt aacagcacca ccggagccat caccagcata
    11461 aagcgaatca gcagcatact gacgcggctg atcccttgct gaaaggcatt cggctcgctt
    11521 tcctgctcac taacacgccc cgccagttga ccaaaccagg tattggcacc tgtagcaatc
    11581 accattgctt gtgccgtgcc gctcaccacg gtggtgccca taaaacacag ggtgtcgcac
    11641 tccagcggat tgctgtgctc cggctggcga gtggtagctg ctttttctac gggcagagac
    11701 tcaccggtta acgacgcctg agcgacgaac agatcccgcg cctgcaagat acgtaaatct
    11761 gccgggatca tatcccccgc cgccagtttt ataatatcgc cgggcaccag ctggtcgatc
    11821 gggatctcca gccagccatt ttcgcctttg tcgttaatta cgcgcagcac cgtcgcagta
    11881 ttgctgacca tcgctttcag ggcatctgcc gctttagtgg aacgtgcttc ctgaataaag
    11941 ttcagcaacg tagaaatagc gaccattagc gcgataacgc ccgcggcaaa taaatcttcc
    12001 gtggcgtaag aaatagcgcc gagaatggtg agtaaaatat taaatgggtt gcgatagcag
    12061 acccataaat gtacccacca cggcgacggt tgttgtgcgg gtaatttatt ttcaccatgt
    12121 tgttcgcggg cagattccac ttccgcctga tttaaccctt ccggatgggt gtcgaacgtt
    12181 ttccataatt cttcttcggg catcaccgcc attttcaggc aatgcgcact taaggacggc
    12241 gggaccgccg tattcactgt ctgctgcgcg ccaggcaatg gatcgcgatg aaccagacgg
    12301 gaaggtaaat ggcgaatgag ccgggtaaaa atttctttaa acataaggag tccctccgca
    12361 ctgtctgaaa cagtacagaa atgtcttcag gcgtggcgaa gccttacctg aaggtaataa
    12421 agctgagcag ggacgttaaa acgtcacggc cttacgcatc cggtaacgcc gtgaaaagac
    12481 gggcttaccg gaaaagtttt aatctccgtc gagggagagg cgtgggatca ggttccatat
    12541 aacctccggt aagtgaaaaa agcgccgcaa ataataagga gaatccaata atattcgtgg
    12601 catctatggc gatataatac ctgctggcaa ctaaaccaac gataaaccag actttaccca
    12661 ttgctgaatg cacgggtaaa gttaggctca aataattaaa caacacgtta caggacaaca
    12721 ggatgcaaaa tcggctgacc atcaaagaca tcgcacgctt aagcggcgtg gggaaatcta
    12781 cagtttcccg ggtgctgaat aacgaaagcg gcgtgagcca gcgcacccgc gagcgtgttg
    12841 aagcagtgat gaatcagcat ggattttccc cttcccgctc tgcgcgcgct atgcgtgggc
    12901 aaagcgataa agtggtcgcc atcattgtta cccgtctgga ttcgttgtca gaaaatctcg
    12961 ccgttcaaac catgctgcca gcgttctatg aacaaggtta cgacccaatc atgatggaaa
    13021 gtcagttttc cccacaatta gttgccgaac atttgggggt gctgaaacgg cgtaatatcg
    13081 acggcgtagt gctgttcggt tttactggca taacagaaga aatgttagcc cactggcagt
    13141 catcgctggt tctgctggcg cgtgacgcaa aaggctttgc ttcggtctgt tatgacgacg
    13201 aaggggcaat caagatcctg atgcaacggc tgtatgacca ggggcatcgt aatatcagtt
    13261 atctcggcgt gccgcatagt gacgtgacaa ccggtaagcg acgtcacgaa gcctacctgg
    13321 cgttctgcaa agcgcataaa ctgcatcccg ttgccgctct gccagggctt gctatgaagc
    13381 aaggctatga gaacgtggca aaagtgatta cgcctgaaac tactgcgtta ctgtgcgcaa
    13441 ccgacacgct ggcacttggc gcaagtaaat acctgcaaga gcaacgcatc gacaccttgc
    13501 aactggcgag cgtcggtaat acaccgttaa tgaaattcct ccatccggag atcgtcaccg
    13561 tagatcccgg ttacgccgaa gctggacgcc aggcggcctg ccaattgatc gcacaggtaa
    13621 ccgggcgcag cgaaccgcaa caaatcatca tccccgccac cctgtcctga tcgtttcctg
    13681 aacgataaat tgtgatcttc gctgcgtttc gggaacgttc ccgtttttaa atttttccgc
    13741 gcaatatatt ctgcagccaa ccaaaaatgt catctgccat ggggctttat gatgagcaaa
    13801 ataaaccaaa cggatatcga tcggttgatt gaactggtcg gcgggcgcgg caatattgcg
    13861 acggtgagcc actgtattac tcgcctgcgc tttgtcctca accaaccggc caatgccaga
    13921 ccgaaagaaa ttgagcaact ccccatggtg aaaggctgtt tcaccaatgc cgggcaattt
    13981 caggtggtga ttggcaccaa tgtgggtgat tactatcaag cactgattgc gtcaaccgga
    14041 caggcgcagg ttgataaaga gcaggtaaaa aaagccgccc ggcagaatat gaaatggcat
    14101 gagcagttga tctctcattt cgcggagatc ttcttcccgc tgctgcccgc gttgattagc
    14161 ggcggtttga tcctcggttt tcgcaatgtg atcggcgatt tgcccatgag caacggtcag
    14221 acgctggcgc aaatgtaccc ttccctgcaa acgatctacg attttctgtg gttgatcggt
    14281 gaagcgatct tcttctacct gccggtcggt atttgctggt cagcggtgaa aaaaatgggc
    14341 ggcacgccga tccttggtat cgtgcttggc gtgacactgg tttctcccca gctgatgaac
    14401 gcttatctgc tcgggcagca gctgccggaa gtgtgggact ttggcatgtt cagcatcgcc
    14461 aaagtaggct atcaggcgca ggtgatcccg gcactgttag ccgggctggc gctgggcgtt
    14521 attgaaactc gccttaaacg catcgtgccg gattacctct atctggtggt ggtacccgtc
    14581 tgttcgctga tcctcgcggt gttcctcgcc catgcgctga ttggtccgtt tggtcgcatg
    14641 attggcgatg gcgttgcctt tgcggtacgt cacctgatga ccggcaactt tgctccgatt
    14701 ggtgcggcat tgtttggctt cctgtacgcg ccgctggtga tcaccggcgt acaccagacc
    14761 acccttgcta ttgatttgca gatgattcaa agcatgggtg gcacgccagt gtggccgctg
    14821 attgcgctgt cgaatatcgc tcagggctcc gccgtgatag gcattatcat ttccagccgc
    14881 aagcacaatg aacgcgagat ctccgtgcct gccgctatct ccgcctggct tggggtcact
    14941 gagcctgcaa tgtacggcat caacctgaaa tatcgcttcc cgatgctgtg cgcgatgatt
    15001 ggttctggtc tggcaggatt gctatgcggc ctgaacggcg ttatggcgaa tggcatcggc
    15061 gtaggcggcc tgccgggaat tctctcgatt caaccgagct actggcaggt atttgcgctg
    15121 gcaatggcta tcgccatcat catcccgatt gtactcacct cgtttatcta tcagcggaaa
    15181 taccgcctgg gcacgctgga cattgtttaa ttttcttcgg ggcgcaattg cgccccctcg
    15241 catttgcagg aataacgtaa tgactaatct tccccactgg tggcaaaacg gcgttatcta
    15301 ccagatttat ccaaagagtt ttcaggacac cacgggtagc ggcaccggcg atttacgtgg
    15361 cgttatccaa cgcctggact atctgcataa actgggcgtt gatgccatct ggctaacccc
    15421 cttttatgtc tctccccagg tcgataacgg ttacgatgta gcgaactata cggcgattga
    15481 tcccacctac ggcacgctgg acgattttga cgaactggtg acgcaggcaa aatcgcgcgg
    15541 gattcgtatc attctcgata tggtgtttaa ccatacctct acccaacatg cctggtttcg
    15601 cgaggcgctg aacaaagaaa gcccttaccg ccagttttat atctggcgcg atggcgaacc
    15661 agaaacgcca ccgaacaact ggcgttcaaa atttggcggt agtgcgtggc gctggcatgc
    15721 ggaaagcgaa cagtactatt tgcatctctt tgcaccagaa caggcggatc tcaactggga
    15781 gaatccagcg gtacgcgcag agctgaaaaa agtctgtgaa ttctgggccg atcgtggggt
    15841 cgacgggttg cgcctggatg tggtgaatct gatctccaaa gacccgcgtt tccctgaaga
    15901 cctggatggc gacgggcgtc gcttctacac cgacgggcca cgagcacacg agtttttgca
    15961 cgagatgaac cgcgatgtgt ttacgccacg cgggttaatg accgtaggtg aaatgtcctc
    16021 caccagcctt gagcattgcc agcgatacgc ggcactgaca ggcagtgaat tgtcgatgac
    16081 ctttaatttt catcacctga aggtcgatta tcccggtggt gaaaaatgga cgctggctaa
    16141 acctgacttt gtggcgttga aaacattgtt ccgccactgg caacaaggaa tgcacaacgt
    16201 ggcatggaat gccttgttct ggtgtaacca cgatcagccg cgcattgttt ctcgctttgg
    16261 tgatgaaggt gaataccgcg tgcctgcggc aaaaatgctg gcgatggtgc tgcatggcat
    16321 gcagggaacg ccgtatatct accagggcga agagattggc atgaccaacc cgcatttcac
    16381 gcgcattact gactatcgcg acgtggagag cctcaatatg tttgccgagc tgcgcaacga
    16441 tggtcgtgat gccgacgagt tattggcaat cctcgccagt aaatcccgtg acaacagccg
    16501 cacgcccatg caatggagca acggcgataa tgccgggttt acggctggcg aaccgtggat
    16561 tggcctgggc gataactatc aacaaatcaa cgtagaagcc gcgctggccg atgagtcctc
    16621 ggtgttttac acctaccaaa agttaatcgc actgcgtaag caagaagcca tcctgacatg
    16681 gggcaattac caggatctgc tgccaaacag ccctgtattg tggtgctatc gccgtgaatg
    16741 gaaggggcaa accttgctgg tcattgccaa ccttagccgt gagatccaac cctggcagcc
    16801 agggcaaatg cgcggcaact ggcagcttgt gatgcataac tacgaagaag cctcaccaca
    16861 accctgtgcc atgaatttac ggccttttga ggctgtctgg tggttacaga agtaaatctt
    16921 ccctaagccc cggtaatgcc ggtcattccg gggttttgtc tgtcagcatg ttgtttttgt
    16981 tgatttttca accagcaaat tcattaaaaa atttacataa cgctgtagcg cccgtcatcc
    17041 gtacgctctg ctttttactt tgagctacat caaaaaaagt taaaacatcc ttgatgcaaa
    17101 gcactatata tagactttaa aatgcgtccc aacccaatat gttgtattaa tcgactataa
    17161 ttgctactac agctccccac gaaaaaggtg cggcgttgtg gataagcgga tggcgattgc
    17221 ggaaagcacc ggaaaacgaa acgaaaaaac cggaaaacgc ctttcccaat ttctgtggat
    17281 aacctgttct taaaaatatg gagcgatcat gacaccgcat gtgatgaaac gagacggctg
    17341 caaagtgccg tttaaatcag agcgcatcaa agaagcgatt ctgcgtgcag ctaaagcagc
    17401 ggaagtcgat gatgccgatt attgcgccac tgttgccgcg gttgtcagcg agcagatgca
    17461 gggccgcaac caggtggata tcaacgagat ccagaccgca gttgaaaacc agctgatgtc
    17521 gggtccatac aaacaactgg ctcgcgctta catcgagtac cgtcacgatc gcgacattga
    17581 acgtgaaaaa cgcggtcgcc tgaaccagga gatccgtggt ctggtcgagc agaccaacgc
    17641 ctcgttactc aacgaaaacg ccaacaaaga cagcaaggtg attccaaccc agcgcgacct
    17701 gctggccggg atcgtggcta aacactatgc acgtcagcac ctgctgccgc gtgacgtggt
    17761 gcaggcacat gagcgtggcg atattcacta tcacgatctc gattactcac cgtttttccc
    17821 gatgttcaac tgcatgttga tcgacctgaa aggcatgctg acccagggct ttaaaatggg
    17881 gaacgccgag attgaaccgc cgaagtcgat ctcaacggca accgcggtaa ctgcgcagat
    17941 tattgctcag gttgccagcc atatttatgg cggcaccacc attaaccgta tcgatgaagt
    18001 gctggcaccg tttgtcaccg ccagctacaa caaacatcgc aagaccgcag aagagtggag
    18061 catcccggac gccgaaggct acgctaactc tcgtaccatt aaagagtgct acgatgcctt
    18121 ccagtcgctg gagtacgaag taaacaccct gcacaccgcc aacggtcaga cgccgtttgt
    18181 aacctttggt tttggtctgg gcaccagctg ggagtcgcgc ctgattcagg aatctatcct
    18241 gcgtaaccgt atcgcaggcc ttggtaaaaa ccgtaaaact gcggtgttcc cgaaactggt
    18301 gtttgcgatt cgcgatggcc tgaaccataa aaaaggcgat ccgaactacg acatcaaaca
    18361 gctggcgctg gagtgcgcaa gcaagcgcat gtatccggat atcctgaact acgatcaggt
    18421 agtgaaagtc accggttcgt ttaaaacccc gatgggctgc cgcagcttcc tcggcgtgtg
    18481 ggaaaatgaa aacggcgagc agatccacga tggtcgtaac aacctcggcg tgatcagcct
    18541 gaacctgccg cgtattgctc tggaagcaaa aggcgatgaa gccaccttct ggaagctgct
    18601 ggatgaacgt ctggtgctgg cacgtaaggc actgatgacc cgtatcgctc gtctcgaagg
    18661 cgtgaaagcg cgcgtcgccc cgatcctcta tatggaaggt gcttgtggcg tacgtctgaa
    18721 tgctgacgat gatgtttctg aaatcttcaa aaacggtcgt gcgtctattt cgctgggtta
    18781 catcggcatc cacgaaacca ttaacgcgct gttcggcggc gagcatgttt acgacaacga
    18841 gcagcttcgc gcgaaaggta tcgccattgt tgaacgtctg cgccaggcag tggatcagtg
    18901 gaaagaagaa acgggttatg gtttcagtct ctacagcacg ccgagtgaaa acctgtgcga
    18961 ccgcttctgc cgtctcgata ctgctgagtt tggcgtggtg ccgggcgtga ccgataaagg
    19021 ttactacacc aacagtttcc acctcgatgt ggagaagaag gtgaacccgt acgacaagat
    19081 cgactttgaa gcgccttacc cgccgctagc gaacggtggt ttcatttgct acggcgagta
    19141 tccaaacatt cagcacaacc tgaaggcgct ggaagatgtc tgggattaca gctatcagca
    19201 tgtaccgtat tacggcacca atacaccgat tgatgagtgc tacgagtgtg gctttaccgg
    19261 tgagttcgag tgcaccagca aaggcttcac ttgcccgaaa tgtggtaacc atgacgcctc
    19321 ccgtgtgtcg gtgactcgcc gcgtgtgcgg atatttaggt agcccggatg cacgtccatt
    19381 taacgccggt aagcaggaag aagttaagcg ccgcgttaaa catttgggga atgggcagat
    19441 aggttaatct tcactctacg ttaagcaaat gccggatgcg gcgtaaacgc cttatccggc
    19501 ctacatggca acaacgattg taggtctgat aagacgcgac agcgtcgcat caggcgttga
    19561 ttaccggatg catcctatac gcggactgat aagccaggcg aaacaatgaa ttatcatcag
    19621 tactatcctg tcgacatcgt caacggcccc ggcactcgct gcaccctgtt tgtctccggg
    19681 tgtgttcatg aatgccccgg ttgctataac aaaagcacat ggcgggtaaa ttccggtcag
    19741 acatttacta aagcaatgga agaccagatt attaacgatc tgaatgacac tcgtatcaaa
    19801 cgccagggta tttccctctc cggcggcgat ccgctgcatc cgcaaaacgt gccggatatt
    19861 ctgaaactgg taaaacgcat ccgcgccgag tgtccgggta aagacatctg ggtgtggaca
    19921 ggctataaac tcgacgaact caacgctgcg caaatgcagg ttgttgattt gattaacgta
    19981 ctggtcgacg gcaaatttgt gcaggattta aaagaccctt ccctgatctg gcgcggcagc
    20041 agcaatcagg tggtgcatca tttgcgatga taagttccct gttacctgag tgcaggcaac
    20101 agggaaatga ggaattaacg atacttctgg tgataggcgt tgcgggtcgt tttcagttgc
    20161 tctgcagcag cctgcgcttc ttttacttta ccttcatttg ccagcttcag cgcgtcgtca
    20221 atctgaccga ccagaatgtc gaaaccgtgg cggaaatctt tcatttccgg gctgtccggt
    20281 gatttatctt cgagcttcgg cggcgttgct ttttgcgcat ccagcgctgc ggcgcgcatc
    20341 ttcgttaacg cgtctttgac ttgcgccgcg ttatccgctt tttcgatcac ttttaaattg
    20401 tcgttgaggg tttccatatt gtcttcaaga tcagcggcaa acgacgcaga actgaatacc
    20461 aacgaggaga ctgcaagaat agctaacagg cttttacgca ttgctcactt ccttttttat
    20521 ttacacttca tccttcacac tgcctcatcg acggctacgc tcattcgccc gggtcacgta
    20581 ctgcgagtac gctccccggc cctcattcac tttccgtctc gatgcagtgc gaatgatttt
    20641 gtgtaatttt ttagtttata gcgcggcagg tcgcgccagt tttttactgc ccggcgattt
    20701 tcatctccgg caacagcaca gaaccacact gtatattact gcgtgtttca atatcgttac
    20761 cgacggtgac aatattgcgc cacatatctt ttaaattacc tgcgatggtg atttcactaa
    20821 cgggatactg aatttcgcca ttctctaccc agaagcccgc tgcaccacgg gaataatcac
    20881 cggtaatggc actcacgccc tggcccatca gctccgtcac caccagcccg gtgcccatct
    20941 ctttgagcat ttgttcgaag cttagccctt gtccggcaat ccgccagttg tgaataccgc
    21001 ccgcatgtcc ggtgcttttc agccccagtt tccgcgccga gtagctggtc agcagccact
    21061 gagtcaggat gccatcttta ataatatcgc gacgctcggt gcgcacacct tcgctgtcga
    21121 atggcgtcga cgccagccct ttcagcagat gcggatgctc ttcaatggtc agccagtccg
    21181 gcagaatttg tttacccagc gaatccagca gaaaggtaga tttacggtaa accgagccac
    21241 ccgctatcgc ccccaccaga tggccaaaaa gcccggttgc cacttcattg gcaaaaatga
    21301 ctggcgcttt catggtggag agtttacgcg gtgacaggcg cgataaagta cggcgagcac
    21361 agtcggcccc gacccactct ggcgtttgca gatcgctcat cgcgcggcca atggtgtagg
    21421 cgtaatcacg ctccatatcg ccattttctt cggcaattac acagctggag agtgaatgac
    21481 gcgtagagca gtaaccctgc aacatgccgt ggctgttgcc aaaaactttg acaccgtagt
    21541 ggctgttaaa actgccgcct tcggtattgg taatgcgttt gtccgcctgc aatgccgcct
    21601 gttctgcgcg ggccgccagt tcaatggctt catccgggga aacttccgca gggtggaaca
    21661 agtcgagatc tggtgcgtca aaggccagca gctctttgtc tgccacgccg gcacagggat
    21721 ctggcgaggt ataacgggca atatccagcg ccgcctgtac ggtgcgggca atggcctgcg
    21781 ggcttaaatc ggtggatgat gcgctacctt tgcggttctg gtgataaaca gtgatcccca
    21841 gcgcgccatc gctattgaat tcaacattct ccacttcacc ataacgcgtg cttacgctaa
    21901 tgccggtggt cttgctgacg gcaacttccg caccgtccga tttgcctgag gccaactcca
    21961 gcgcagtgga aactgcttct tccagaatct tgcgctgcgc ttcaacttga gagattactt
    22021 tcattgcaag tgccataatg tagagagttt ctctgaagtc taacagagaa ccgtttttca
    22081 gtgcgcatct taactggtaa cattagcctc tttttttaag gagcctgaga tgactaagca
    22141 gcccgaagac tggctcgacg acgttcccgg tgatgacatc gaagacgaag acgatgaaat
    22201 tatctgggtc agtaaaagtg aaattaaacg tgatgccgag gagctaaaac gccttggcgc
    22261 ggaaattgtt gatctgggga aaaacgcgct ggataagatc ccgttagatg cggatctgcg
    22321 cgcggctatt gagctcgccc agcgtattaa gatggaaggt cgccgccgcc agttgcagct
    22381 catcggtaag atgctgcgcc agcgcgacgt agagcctatt cgtcaggcgc tggataagct
    22441 gaaaaaccgt cacaaccagc aggtggtgct gttccataaa ctggaaaacc tgcgcgatcg
    22501 tctgatcgat caaggtgatg acgccatcgc tgaagtattg aatctgtggc ccgatgccga
    22561 tcgtcagcag ctgcgtactc tgatccgtaa cgcgaagaaa gagaaagaag ggaataagcc
    22621 gccgaaatcc gcacgccaga ttttccagta tctgcgcgaa ctggcggaga atgaagggta
    22681 attttcgttg ttgatgtgag tatggtttgc cggatgcggt gcacctgtag gccggataag
    22741 acgcatcaag cgtcgcatca ggcactatgc tcaaatgccg gatgcggcgt aaacgcctta
    22801 tccggcaatg ctatctcagg ctgaggccga attactgcgc agcttccgcc ttcttcgcca
    22861 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatg accagaatat
    22921 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    22981 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    23041 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    23101 tccccatttt catggtattc gagcgcggct ccagcacagc aataatgcgc gccgtaccac
    23161 caactttgcc acgcagcgcc gcaagcgttg ccagaattgc cgtcgggtga tgggcaaaat
    23221 cgtcatatac cgtcacgcca ttcgcttcac cacgcaactc cagacggcga cgagcgttaa
    23281 taaacgaacc cagcgcgtta gcggcatctg caggcgctac accaacatgg cgagccgccg
    23341 caatcgccat caggccattg tgcatattat gttcgcccac cagcgaccat ttcacttcgc
    23401 ccactttttc gccatccagc aagacttccc attcagaagc gtcagtggtc agctttttcg
    23461 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    23521 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    23581 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    23641 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    23701 tgtcgaagaa ggcgcagtca tactcatccg cttcgataac aaagaagtcg ctttcgccca
    23761 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    23821 cgcactgttc cagaatccag gtcgccattc ccgcggtggt agttttgccg tgtgtaccgg
    23881 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    23941 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    24001 tgccaataat caccagatcc ggctgcggat cgagctggct ggcatcgtaa ccctgaatca
    24061 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    24121 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    24181 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    24241 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    24301 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    24361 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    24421 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    24481 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    24541 ctgagaacgt gcagggcgag gttcagcaga aactcgactt gttcgctaat gaaaaactga
    24601 aagccgcact gaaagcacgc gatatcgttg cgggcattgc ctctgaagaa gaagatgaga
    24661 ttgtcgtctt tgaaggctgt gaacacgcaa aatacgtggt gctgatggac cccctggatg
    24721 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    24781 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    24841 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    24901 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    24961 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    25021 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaaccgcc
    25081 cgtatacctc acgttatatc ggttcactgg tcgcggattt ccaccgtaac ctgctgaaag
    25141 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgctgt
    25201 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    25261 aagagcgtat tctggatatc atcccggaaa ctctgcacca gcgccgttcg ttctttgtcg
    25321 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgaattcccg gacgcgtaat
    25381 ctcctgcgcc tctccacacg gagaggcgtt tttatcgctg cgcaatattg actcttgtca
    25441 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaag gcaataaaaa
    25501 tgaacagcaa aatgccgatg gcgattttcg tccaccagga actcagcgta ccgtcaaagt
    25561 tgatataagt ctgaattagc ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    25621 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagttcca
    25681 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    25741 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcgg gtagaaatcc
    25801 ccatcaggtt tgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    25861 tacgatgcgc gaggaatatg ccgataacca ccaccgccag catcagcagt cccatcgcac
    25921 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    25981 ggtttatcgg gatcgactct tctgaaacga gatagctgac gccgcgcaaa aagaacattc
    26041 ctgccagggt aatgataaat gccgggatct tcagggcgtc gatcaaaagc cccataaatg
    26101 cgccaaaggc acagcccatc accagcacta gcggaaacgc cagcagcggc gagaggccga
    26161 aatcgccaat cacttttgcc agaaacacgc cagtaaaggc gatcaccgaa ccgacggaga
    26221 gatcgatacc gccagagagg atcacaaagg tcatgccaac ggcgattatc ccaagaaagg
    26281 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccggga aactgggtca
    26341 ggcagtagag atagcccagc acaaagacgc cgatggtgat catcagcggc aagttacgtt
    26401 ttatcatggc tacgtactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    26461 acacaaagca ccaccaccgc ttttacgacc tggttcatct ccggcggaaa gccagaaagt
    26521 aaaattccgg tgttcatccc ctgaataatc agcgccccca cgactgagag cagcaggtta
    26581 aagcgcccgc ccatcagcga tccgccgcca atcaccaccg caagaatggc gtccagctcc
    26641 agccataacc cggcgttatt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    26701 gcaatcgccg cacacagccc gctcaacacg taggtgagca tgacgatgat ccgcgtgttt
    26761 accccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    26821 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    26881 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    26941 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    27001 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgcc
    27061 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    27121 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ctacggagag atcaatccca
    27181 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    27241 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    27301 ggggctacca ggctatcgac cagcagcacc agcaatagcg ccgccagctg cggcattccc
    27361 gttggccagc gaaagcgcct tttcggcggc gtagtgtccg ggagagattg aggcatcaca
    27421 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct ctgccagcgg
    27481 gatctccgcc acctgtttgc gatcgcgcat gataatcacc cgatcggcat agcccaccag
    27541 ctcttccagt tcggaggaga tcaccaacag cgccagaccg tcagcacaca gcgtttcaat
    27601 caggcggatg atctcggcgt gggcaccaac atcaatgccg cgggttggct cgtcgaggat
    27661 cagaaattgc ggtcgggtca gtagccaacg tgaaagcaac actttttgct gattgccgcc
    27721 agagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    27781 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    27841 ctggagagcg aggatgatat tttcccgcac cgaggcggca gcgatgatgc catcggtttt
    27901 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    27961 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    28021 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    28081 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    28141 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcgcgtggg tatccagctc
    28201 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    28261 tacgaaactg ccgttgcgta agacggtgat ccgatcgctg acctgataga cctgatcgag
    28321 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    28381 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    28441 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcca ccgaaaaacg
    28501 gttaagcggt tcgcgcacgt cgagggagaa accgtaagat gccatcagtt cggtggcgcg
    28561 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcggc ctataaatag
    28621 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccgatacc
    28681 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    28741 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    28801 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    28861 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    28921 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    28981 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    29041 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    29101 actcttcttt tgcggtatca ggcagataaa gggtggattt ggtcagcgtc agcttttcag
    29161 gcatggtgcc gtcttttttg tatttctcca gcgcgtcgaa ggcgggacct gccatattcg
    29221 gcgtcagttc aacactggcg ttcgcttcgc catcaatcat cgctttgtag atatcgggta
    29281 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    29341 cctgaattgc gccaatcacc atgtcatcgt tatgggcgta aaccatgcag atgtttttgc
    29401 cgttgttttc cgctttgata aagctctcca taacttcttt gcctttactg cgggtgaagt
    29461 cacctgactg cgagcggatg attttgatat ttggcgcatt cttaatggct tcggcaaagc
    29521 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagttcc accacgttgc
    29581 atggtttgcc attcacttct tttaccagcc agtcaccaat caacttgcct tcgaggatgt
    29641 tgtcggcagt gacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcaa
    29701 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    29761 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    29821 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    29881 cggcctcact tttcgccaca ttggtttctg cagcgcgcca gccggattcc gatccgacct
    29941 gcgaaaatcc tacggttaat ggagcggcca acgccataga cgacatggct gccgagactg
    30001 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    30061 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    30121 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    30181 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    30241 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    30301 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    30361 gggtaaagat ctgccggaag acatctacgt ggttattgag atcccggcta acgcagatcc
    30421 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    30481 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    30541 cccggttgac gtactggtcc cgactccgta cccgctgcag ccgggttctg tgatccgttg
    30601 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    30661 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtta acgatctgcc
    30721 agagctgctg aaagcgcaga tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    30781 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    30841 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccgag
    30901 cttcggttag taagggtttt tttatgcccg cgataaataa acacacctta ttccaccacc
    30961 gcctgcaagc gtaataacgc ctcttccacc acctcatccg cagccagacc aatacgcttt
    31021 gccagccggg cgcgtagatc catcatccgc acctgattca ccagcaccac gccgtgcaca
    31081 tcgccttctt cgcaatgtaa aggaacgcta aatccggcat aacgggcaaa atttccgccc
    31141 tgcgtaatgg gggccaccag cgtcattccc agttgattaa aggcttgaac ggagagcaca
    31201 agcgcaggtc gaccagcacc ttgctgttca tggccgcttg ctggatcaaa gccaaccagc
    31261 acaatgtctc cccgttcaaa ttcacttttc tttaccatat ttcgtcaccc gcaggggtgg
    31321 atttacccca gacatcctgc tcgctaagtt ccgcggcgtt catgtcacac tgtgccagca
    31381 gttcatcaag cgagtagcgc ctggagatgg gtgtcagaat cagttgattg ttgctcactt
    31441 gcgcctccac gctctgcccc ggctgtaagt taagttcttt cattacgata ttgggaatga
    31501 ccatacctgc actgttcccc catcttttta tggtaatacg catctgcacc tccttaagtt
    31561 atacaaagta taactcacgg tgctacagga aacgttttac ccctgcgcat ttgtgaacgc
    31621 cagcagagtt ttttatgcgt taacagatat ggaaagcggc tcggagagaa aagcaaaggg
    31681 tgagggaatt acaaaacaga atgcgagtcg tctcgcaaaa aacaacgcca cccgaaggtg
    31741 gcgtatgcat atggttactt atccctgtct aaccagtcgc cgctttcaat tagctttaat
    31801 ccatcgacgg gtcgttgata aacgtacatc catgcactcc cgtacggcgt ctgaatcaac
    31861 tggcgcgcgt attcaccgcc cctggtgcgc aaggcatcaa gttcggccag cgtggcgttg
    31921 tcaatacgat aaacttcacc gtgtactgtt ccgttccccg gaactgcgcc tggatagtgg
    31981 cccaggctat acaactggta gttatcgata ctgaaatcgc ccagtaactg ggcattggtc
    32041 atccagtgac tgttgccttg tttgtggcgt aaactgccgt agacaaatat tcgcattgct
    32101 aaaactcgaa ctgatagagc aaatccagtg cctggtctac accagacacg gcttccagat
    32161 atagcttagg catcaggcga taacgtaacg tgagtgttgc tatagagtca aatataccca
    32221 cgccgtattt cacttgcaga cctggcaata catagccgct gactactacc tgggaggagt
    32281 cgcctactcc ctgggtgtcg agcgctaaat tgcttacgcc aaacgtctcg ccgattttac
    32341 ccacaatctg gccactttgc gcaaccccca gaccaatcag catcgaggtc attgccgcac
    32401 tgtcgctctg atcgctctcc agcccttgtc cacgtagcaa ataagacaat gcagcttgtt
    32461 gcgacatcgc cgggtcagag aagatctccg ctttcggttc gtccgccaga ccagtgacgc
    32521 gaactccggc gattacgtcg tcttctgtag catccgggtt acgaatagct tcaatattaa
    32581 gatacggttg atccggcgga ccagagaaca gtaactcacc cttacgcaca atcagatcct
    32641 gaccataggc atggaagcgc ccttcaggga tgttgatctg cccgttcagg cccagccctt
    32701 gtttgtcctg aacgacgtta agatcgcccg tcagccgcgc tttcaggcca aaggcgtcaa
    32761 tgcgcacatt gttgccaacg tggacaatca ggttactgtt aatcggaatc gacgccgttt
    32821 tcggctcttc cggttgcagg ttatcgttaa gcatcaccac atcgctggag acgcctactg
    32881 cgctttccgg cagatcgtgc accacgatgc gcgcccacgg gacatccacg cgaccatcga
    32941 gggtaaacag gtttggtgta gcctcgaata caacatctgg cgatacatcc attcgtacca
    33001 tcggcggcac ggtgatccgc actttactgc ctttcgccgt tactcgtgcc cgccagtttt
    33061 caatttggct ccagtcggca tcaccgttca ggtagatttc accctgctgg gtccgtactg
    33121 taccggcaag cgtcgagcgc ataccgttaa agttgaccgc aagctggctc ggctgcatat
    33181 caaacggcat aaagttgccg tcgatatcca cacccgtaac ctgaagctga ccaaacaact
    33241 gcgggctttg cacatcacca cccagacgca agttggcact caccatcccc gctgcttttt
    33301 ccccacggtt aaagatgggg tttatcatcg caaggttgaa gttacggata ttgacgttgc
    33361 caccaagatt acggcggcct tgcggatcgg tcacctgcac ctgtccatca aactggccgt
    33421 tattggtcag gcggatggtc cagcccaatt cggcacggtt gttacgcaat tccgccgtca
    33481 gattcagtgt ctgaaacgcc acgggcagcg ccgcatcgtt gacggtttgc gttacctgca
    33541 cgttacgccc cgaaagggtg atactgccct gcggcagccc ctctttcgtg gtgtcccagg
    33601 caacatccgc tttacccgtg aagataccgc tggcctgagt ggtttctggc ataaacggtt
    33661 tcagcatggc gaggtcgaag cggttgagat tcaccaccgc acgcccttcg gcccccgcat
    33721 cgatagtttg cggcacgcac agttccgcat tcgggttaag ccagcagtgt ggcccgatgc
    33781 tgattttttg ctccttattg cggtaatcca gcgcaatatc gcgggtcagc gaccacgggc
    33841 cgaccggcgt ctggaagcgg gtattgctaa gagttccctt ccagcgttct tctttgcgat
    33901 caaaacttcc tgccagatta agctgcccgg agacaggctc gccctgaatc cgcaactgta
    33961 gctcgtgctg cttttcgctg cctttggcat tcagggtgac gaggttgata tttacatccg
    34021 gctgcgaaat ttgctcaacg cgtacgtcga gtttcccggc gatctgatcg gtggatttaa
    34081 tgtcgccttc cacgcgaacc tgcgccacgg aaagttcctg ccagcgcagg ccgcgcgcgg
    34141 tgatatctgc cagtagttgt ggcgcttcca ccgtgccgcg tactttcacc agccctttcg
    34201 ctgtaccgcc aagccccggc agcgcgttat ccagccccgg cgcgttgatg gtggcatcaa
    34261 gattgagatc ttttaccccc agctcgcctt tcacttcggc actgtttggc ccgagttcca
    34321 gatgcagccc tgggatcatc cactgcatat aactgttgcc tttcagcgtg ccgtcaacgt
    34381 tcactttgtt ctgtttgacg ttaccggtca gcttcagttc tggcacctcc atctgccagg
    34441 tgccgccgta caggctaccg cgggttttaa tcaagccatt gagtttcgac ggccactccg
    34501 ggaactcttt ggcggtgtta atgccattaa gcgttagctc accgcgccaa ctaatggcct
    34561 gctgccagtc gagcaacgcc ttgagttcag ttttcccttc cagcgccgcg acggtgagtt
    34621 tgtcgagatt gacctgctgt tcattacctt tggcatcaag ggtaatggtg gctggcggga
    34681 tctcctgtcc cttcactgcc gtacgcatag agagcgtgta atcggtcatt ttgccggtaa
    34741 gtttcagttt cagatcatcc gcctgatact gcttctcacc agtgaacggc cagtaaagct
    34801 gtttgctgtt cacttccacg ttgagcggca atccggcttc cgccagtcgc gtctgggcgc
    34861 gtaaatccat atccaccgga ccggaaaggt taacgccaat ctccagctgt tcgcgcagcg
    34921 caccgcccac tttcagcttc agtttttctc ctttcaacgg ctccacgttc agtgtgctgt
    34981 tgagggtgat atccaccggc cagttgtctg acagctgcgc cgtgccgctg gcgttgacga
    35041 tcccttgact ggaatcgata tccagggcgt ccagtttagt attgccgtca atgctgctca
    35101 ctttcagcag catggtgcgc acggtgatgt ccgtgtcgcc cgtcacgcgt aactgctcgc
    35161 ctttaaactc ttcaatgttc aggttaagcg gcaaatgcac gtcggtcatt tccggcaata
    35221 ccgggcgaga aaagagatct ttcagcgttt cgccgagcgg cttttcctcc ggctgcggat
    35281 tttcaatttt cggttcgacc acttcttcct gcgccacttc cgccactttc ggcagagcaa
    35341 tcagcaggcc tttcagcgac gtcggtttca gggtcagggt tttctcctgc cagttcaggc
    35401 cggaggtgaa gtccatcacc gacaccgtgg tgtcatcaat cttgatgttg acgttgtcca
    35461 gcgccacccg tgtcagggtg atgggatacg gcgtggagag atccagcgga ccgctatctt
    35521 cctcttcttc aacctgttca gaaggaggca tttttttact gtcgatgttg acctgaatgt
    35581 ctttcagcgc caggtcatta atacaaacgc tactgttcca caggcactca agaccgaccg
    35641 ccagatgcag attacccgct tttaccgcca cgcctggctg ctcataacga acgtcagaca
    35701 aggtgagatc gcgccagccg ccggtgacct tgccaatatc cagtcctggc acccagcgat
    35761 ccgccgcttt aaatacaaga tgcaggccgc tggtggtgcc caccagaaac gccaccgatc
    35821 ccagcaacag taagataacg ataaccacgc cgaggctgat ttttttccat aaactcataa
    35881 ttctggcccc agaccgatgt aaaactgtaa cccgtgttcg tctttatccg cgaccggtac
    35941 ggcaaaatcg agtttgattg gcccgaccgg cgattcccag cgcacgccga ccccggtacc
    36001 ggttttaaag tcgctgcggc gaatatcgct taccgcttcg ccactatcga caaacaccgc
    36061 gccccaccat tttccggtca cgttgtactg gtattccagc gatccggtta tcaacttcga
    36121 ggcccctttc aggtcaccgt tggcgtattt cggggcgata gatttgtatt tgtagccgcg
    36181 aatactgcga tcgcccccgg cgaagaaacg cagatccggc ggtactttgt cgaaatcacc
    36241 ggtttcaatc cagcccagcg tgccgcgtgt aacgaaacga tggcgatcgt acagtgtgcg
    36301 gatccagacg ttctgcgcct ggaaaacgga gaaatcgaca tctgaacccc aggccgtgtt
    36361 ggagtagtcg atagagtagc gttgcgagtc gccccaggtt ggcatcaggc caccacgaga
    36421 acgcgtgcgg ctaatcatca ccccaggata aaacagcatc gtggtgttgg taatttcacc
    36481 ctgggtaaag tggtcgaggc tccagcgcag gttaatggca cgctgccagc cgctggagag
    36541 atcccagtag cgagaagcca ccagcgtagt ggagtcagat tcggtatcgt tcaggtcagt
    36601 gcgcttaaaa ccgccctgca ccaaataata ttgttccagt ggattcttca gcagcggcat
    36661 tttatagctg aagtcgaggg tctgttccgg cgcggaaata ctggtactgg tggtcagact
    36721 gtgaccataa gagttcatcc acggcttttt ccacgtcgct ttcacgcgcg gtcccacgtc
    36781 cgtagagtaa ccgaccccgg tttcgatggt gttttcagtt cgcggcgaaa ccacgcccgt
    36841 caagggtaat actttcgttt cgcgcgcttt atcaaattgt ggagccacca ccaccgagtt
    36901 aaaccagccg gtagcagaaa gtcggcggtt cagttccgcc agatctttcg attcgtactc
    36961 atcgccctct ttaaacggca ccagattttg caggtattca tcgcggattt gtgatccttc
    37021 aaaggtcaca tgcccaaagc ggtaacgttc gccactgtta taatcaatat cccagaaggc
    37081 tttatgcagg ccgagcgcaa tgcccagctg cgctttggta aattcgctat cgaaataacc
    37141 tttacgcaac gcaatgctgg ttaaggactt tttgaaattt tcataatcgc cctggttcag
    37201 caccgtgcca atagccgggc gagtatcgag caatttcaaa tagtctttat cggtccgcgc
    37261 gccgccgcgc aataccacat cggtgccgcc aattaacacc ggcacacctg gcgtgacttt
    37321 ggcgatcaat acctgccgcc ctttctttgg cggtggacgg agatcaaatt caatggtcgg
    37381 ctggtaataa cccagcgctt tcagaccttc gcggatggca tcatcgacgc gtgcgcgaaa
    37441 gcgacggtct ggcgtcactt catcactttc aatcgtagaa agctgcgcac gaacgttctt
    37501 ttccagctgt cccgataacc cctcgacctg tagacggacg ttcgcggcga cggcagatcc
    37561 gcttaagcag agtaagctta cacagcataa ctgtcggata tagcgcacat tttctcctga
    37621 atatcctttt ttcctgcccc tggaacgccg ttaaacggct taacaaaaat ccagtaatat
    37681 ggattaaaaa gcagactaaa ccccaaatat ttcttatgtt ttactttaga cctattcacg
    37741 gtggttattg tgtgcaaata cgcctcttgt tacaacctta accccaatga ccgattttcg
    37801 ggagagcgac accatgagtt tatttgataa aaagcatctg gtttcccccg ccgatgccct
    37861 gcctggacgt aacaccccga tgcccgtagc cacgctgcat gcggtcaacg gtcactcaat
    37921 gaccaatgta cctgacggaa tggagattgc catttttgcg atgggttgtt tctggggagt
    37981 ggagcgtctg ttctggcagt tacccggcgt ttacagcacc gccgcaggct ataccggagg
    38041 ctatacgcca aatccgactt atcgggaagt gtgctccggt gatacgggtc atgccgaagc
    38101 ggtacgcatt gtttacgatc cttccgtcat cagctatgag cagttgctac aggtattttg
    38161 ggagaatcac gatcccgccc agggaatgcg tcagggcaat gaccacggca cgcagtatcg
    38221 ttcagcgatt tatccgctga ccccagaaca ggatgccgca gctcgcgcca gtctggaacg
    38281 ttttcaggcg gcgatgcttg ccgccgatga cgatcgtcac atcaccacgg aaatcgctaa
    38341 cgccacaccg ttttattatg ccgaagatga ccaccagcaa tatctgcata aaaacccgta
    38401 tggttactgt ggaattggcg gaattggcgt ctgtctgcca ccggaagcat agcgttacgg
    38461 gtacaaatgt agattgttga taaagtgcgc tttatttatg ccagatgcgg cgtgaatgcc
    38521 ttaccaggcc tacaaaatcg tccaaattca atatattgca gggactgcgt aggcctgatc
    38581 ggcatagcgc atcaggcagt attgcattta tcagcagtct gaattttaac cctctggcga
    38641 ctttacagta ccttacgcta tactagccac tgaaaatgcc ggttcacttt cttcgaatcg
    38701 gctttcaatg tgtatttcac acaaattaat caacttcccc ttccgaggat ctggcctgaa
    38761 aggtcggata agatatgtta aacagtattt tagtcatact ctgcttgatc gctgtaagtg
    38821 cgttcttctc gatgtctgag atctcacttg ccgcctcacg caaaatcaaa cttaaactgc
    38881 tggctgatga aggcaatata aatgcccaac gcgttctgaa tatgcaggaa aatcccggca
    38941 tgttctttac ggtggtccaa atcggtctga acgcagttgc gattctcggc ggtatcgtcg
    39001 gtgatgcggc attttctcca gcttttcaca gcctgttctc ccgctatatg tcggcagaac
    39061 tctctgagca actgagcttt attctctctt tctcgttagt gactggcatg tttatcctgt
    39121 ttgcggattt aaccccgaaa cgcatcggta tgattgcgcc agaagcggtg gctttgcgta
    39181 tcatcaaccc gatgcgcttc tgcctgtacg tttgcacccc gctggtgtgg ttcttcaacg
    39241 gcctggcgaa catgatcttc cgtattttca aactgccaat ggtacgtaaa gatgacatca
    39301 cttctgatga catctacgcg gtagtggaag ccggtgcgct ggcgggcgtg ttacgtaaac
    39361 aggaacacga gctgattgaa aacgtctttg agctggaatc ccgtaccgtt ccgtcttcaa
    39421 tgacaccgcg tgaaaacgtg atttggtttg atctccacga agatgagcaa agcctgaaga
    39481 ataaggtggc ggaacatccg cactctaagt tcctcgtctg taatgaagat attgaccaca
    39541 tcatcggtta tgtcgattct aaagacctgc tgaaccgcgt gctggctaac caaagcctgg
    39601 cactgaacag cggcgtacaa attcgcaaca cgctgattgt gccggatacg ttaacccttt
    39661 cagaggcgtt ggaaagtttt aaaaccgcag gtgaagactt cgcggtgatc atgaacgagt
    39721 acgcgctggt ggtggggatc atcaccctca acgacgtgat gaccacgctg atgggcgatc
    39781 tggtcggtca ggggctggaa gagcagattg tcgcccgtga tgagaactca tggctgattg
    39841 acggcggcac cccaattgac gacgtcatgc gcgtgctgga tattgacgag ttcccgcagt
    39901 cgggcaacta cgaaaccatc ggaggcttta tgatgtttat gctgcgtaag atcccgaaac
    39961 gcaccgattc ggtgaaattc gccggctaca aatttgaagt ggtggatatc gataactacc
    40021 gcatcgacca gctgctggtg acccggatcg acagcaaggc caccgccctt tcgccaaaac
    40081 tgcctgacgc taaagataaa gaagaaagcg tcgcgtaacc cccagaaaca tcaacggctc
    40141 ctgaatcagg agccgtctta ttactgcata gcactttggt taagccatct ctgtttgcag
    40201 acgcataacc tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt
    40261 tgggataaga atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg
    40321 tccacggaac aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat
    40381 tttcattgtt atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat
    40441 tatgaggcgc aaattttaat gcctagtgac tatagactat ccgggcaatg tttccaccgc
    40501 gtataacttt ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg
    40561 gtcttcagta taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc
    40621 acacttgcgg gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg
    40681 atcacctgca tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc
    40741 tgcctgctgt tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg
    40801 ccgattggca tcaccgatcg tggcgagttg gtgcttgata aagatcagtt tagctacaaa
    40861 acctggaaca gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc
    40921 acctctgcaa aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg
    40981 cacgatcgtt accagaccac caccattgtt aacaccgacg acgcaattcc gggttccggc
    41041 atgtttgtgc gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt
    41101 gttgatagca atggcgtcgc acgcggtgcc tggcagctgg atgaaaaaag ttccgctgta
    41161 gtggtgctgg ataaagacgg tcgcgtgcaa tgggccaaag acggggcgct tactcaggaa
    41221 gaggtgcagc aagtgatgga cctgctgcat aaattaatta ataaatagac actctgaacc
    41281 caggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc cagtcgtgaa
    41341 cgtgcgctcc ggcagctgcg gccacagcat gtccagcggc ggtgtcccaa atattcgttg
    41401 gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat ttcagcgaag
    41461 agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc agctccgcat
    41521 ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc tgaatctgct
    41581 tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac attacgttca
    41641 ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc gcaatgttaa
    41701 cggtgaattc gccattgcgt ttaataaact ctttagtacc atccagcggg tctaccagcc
    41761 agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca gaaaggaccg
    41821 gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg gcaatatccg
    41881 ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc gtcccgtcgt
    41941 agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga tctaacattt
    42001 ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc tatcgttaat
    42061 gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg cattcttcac
    42121 tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg ataaaaggat
    42181 gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg ccagtgtgaa
    42241 tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca acatgatgga
    42301 tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta cggcaagcct
    42361 gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg gcgatttgat
    42421 tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaagcag gtgatattca
    42481 cccagtgtat aaggcattaa atacgctgga ctataccgtc ggcacgcttg gcaaccatga
    42541 gtttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat tcccttatgt
    42601 aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt atttaattaa
    42661 agacaccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg gctatattgg
    42721 cgtcgtaccg ccgcaaatca tgggctggga taaagctaat ttatccggga aagtgacggt
    42781 gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga aaggtgccga
    42841 tctcgttgtc gttctggcgc attccgggct gtctgccgat ccgtataaag tgatggcgga
    42901 aaactcagtt tattatctca gtgaaattcc gggtgttgac gccattatgt ttggccatgc
    42961 tcacgccgtt ttcccaagta aagattttgc tgatatcgaa ggagctgata tcgcgaaagg
    43021 cacgctgaat ggtgttccgg cggtaatgcc aggcatgtgg ggcgatcatc ttggtgtggt
    43081 cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga aagcggaagc
    43141 tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca gcaagctggt
    43201 agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc caatcggtaa
    43261 atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga ccgtgcaagt
    43321 agtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc cggatctggc
    43381 aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca aaaatgaccc
    43441 ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg ccgatcttta
    43501 tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga aagagtggct
    43561 ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac cgcaatcact
    43621 catcaactgg gatggtttcc gcacctataa ctttgatgtg attgatggtg tgaattatca
    43681 gattgatgtt acccagcctg cccgttatga cggcgagtgc cagatgatta atgccaatgc
    43741 ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg ccatgttcct
    43801 cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg gcgacagcca
    43861 tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga ttgctgatga
    43921 gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt tagcaccgat
    43981 tgctggcgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata aagccgcagc
    44041 gtttattaaa gagaaagggc aatatccgat gaataaagtc gcgaccgatg atatcgggtt
    44101 tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct ataaatcatc
    44161 aaccgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg ccctacaagc
    44221 catgcaccgt agaccagata agctcagcgc atccggcagt tatgccgcac gttcatcccg
    44281 caccgccaac acctcgggca aattcaactc aatccagtcc gccagtgcag caaccttttc
    44341 gctcacctgc tcgcccagcg gcgtgaggct gtattccaca tgcggcggca ccaccggata
    44401 cgcgatacgg ttaagaaaac catcctgttc taacgcctgt aacgactgcg caagcatctt
    44461 ttcactcacc ccaccgattt tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag
    44521 cgccaccaga atcaataccc cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg
    44581 gcactgttcc gcaaagagat taccctcttt cagttgttgc gacaggctaa cctgactcat
    44641 ttcatactta cctttttgta cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa
    44701 acacaagaca aaacgatgga gacttcccat gatcgctatt actggtgcca ctggccaact
    44761 tggtcactat gttattgaat ccttgatgaa aacggttcct gccagccaaa tagtggctat
    44821 cgttcgtaat ccggcaaaag cccaggccct ggcagcacaa ggcattaccg tgcgtcaggc
    44881 tgactacggc gatgaagccg cactgacatc tgcacttcag ggagtggaaa aactactgct
    44941 gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc
    45001 aaaggcggct ggcgtgaaat ttatcgctta taccagcctg ctacatgcag atacctcccc
    45061 gctcggcctc gccgatgagc acatcgagac ggataaaatg ctggctgatt ctggcatcgt
    45121 ttacaccctg ctgcgcaacg gctggtacac cgaaaactac ctcgccagcg ccccggcagc
    45181 actggaacac ggcgtattta tcggtgcggc gggcgatggc aaaatcgcct cagcaacgcg
    45241 ggcagattat gcggcagctg cggcacgcgt gattagcgaa gccggtcacg aaggcaaggt
    45301 ttacgaactg gcgggcgata gtgcctggac gttgacacag ttagcggcag agctgaccaa
    45361 acagagcgga aaatccgtta cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact
    45421 gaaaagcgtc ggactgcccg acggactggc ggatatgctg gcggattctg acgttggcgc
    45481 atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc aaattgattg gccgcccaac
    45541 gacaacgtta gccgaaagcg taagccatct ttttaatgtt aataactagt taattaaagt
    45601 ggcatcctcc cgcatcctct ctaataatga cgggatgccg ggagcaatca tgtctgcttc
    45661 ctgaactttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    45721 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt accggaatac
    45781 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctgcccatt
    45841 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    45901 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    45961 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtcat tcctgtcttt
    46021 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    46081 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    46141 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    46201 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    46261 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    46321 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    46381 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    46441 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    46501 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    46561 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcattgc ggtcagagca
    46621 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacacgctgg cagtgagtta
    46681 aataagcctc tgctacgtaa gggttatagc ttttgcctta aagatgcatt taaaatacat
    46741 cttatattat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    46801 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    46861 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    46921 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    46981 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    47041 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    47101 tgccaaaagg gctgacaaaa tacctgacta tgctgcatga agagctttcc agccacatga
    47161 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    47221 gaccaatcag cgtgatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatta
    47281 aacacaccac caataacgtc acaccgccgc cggaagcctg caccacctgg aaagcgatgt
    47341 ataacggtat taatgaactg attgatgacc tgatggatca catcagcctg gaaaacaatg
    47401 tactgttccc acgcgcgctg gcgggtgagt aatctaaaag ctggatggca ttgcgccatc
    47461 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacaacc
    47521 agcccagccc cagtgcgata aaccacagcg gggtgaccaa cagcgcctgg cgagtgtcat
    47581 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    47641 gcttgccgag cggcatcttg tagatcgact tctcatgcag atgaggacgc tgtttgcggt
    47701 aaaccaggta cgagcaaagg ataattgtcc agacgaacat aaacagaatc gcggaaacgg
    47761 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    47821 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    47881 tagcgaacgc tttcggtgct acaccttcct gcgccagacc aaacagcata cggctggtag
    47941 agaagacgcc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    48001 ttgcggcagc aggcagccct accaacacga acagttcaac aaacgggctt ttctccggga
    48061 ctaccgaact ccacggcgtc acggacataa ttacaatcag cgcgaagacg tagaacataa
    48121 tgatacggat cggaatagag ttaattgcac gcggcagtga tttctccgga tctttggttt
    48181 ccgcagcggt tgtacctacc agctcaatcc ctacgaaagc gaaaaccgct atctggaatc
    48241 cggcaaagaa gccacttaaa cctttcggga accagccgcc atcattccac aaatgcgcga
    48301 atgacgcttc cacaccagtc ggtgactgaa agtgcatcgc caccataacc aggccgacga
    48361 caatcagcga cacaatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    48421 acattttcac ggtggcgaga ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    48481 cccagtcgga gagatcgggg aaccagaact gagcataagc agtgatcgcc accacgtctg
    48541 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cctgcccacg
    48601 gcccgagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    48661 attcccccat tgcccgcatc acgaaaaaga gcataaaacc gatgatcata taaacgaaaa
    48721 tgatcgacgg cccggcaagg ctaatcgttt tgccagaccc cataaacaac cccgtaccaa
    48781 tggcaccgcc aatggcaata agctgaatat gtcggtttgt gagattgcgc cgtagcgact
    48841 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg tttttttctt
    48901 cctgtacctg tctgtgttgt tcaggctcta cggcctttag tcagtctatg atacgacgaa
    48961 tctgtatcgg gactcatcga tattaggtaa caatacgcgg gatgaatact aagatttaga
    49021 tctaatgtta attttatgtt taaagtgagt gtcatatcac ggtgataacg cgaaacagct
    49081 cacaaaaata cacgcttacg gggtctttgc aacacaaaat agcgccgttt ggcaaactga
    49141 agggtttatt gctgaatgcc tgctcccctc tggttcgagg ggaacagaat gcgctgctta
    49201 gaggatttcc agcagttcga cttcaaacac cagggtgctg aacggaggga tggatgcgcc
    49261 tgcgccgcgc tcgccatatg ccagttcctg cgggatagtc agttcccatt tagaacctac
    49321 cggcatcaga gtcagtgctt caatccagcc agggatcacg ccattaaccg gaaattcagc
    49381 gggttcacca cgagcaacgg agctgtcaaa cacggtgccg tcgatcagtt taccggtgta
    49441 atgaacacga acgcggtcgg tacgtgccgg aattgcgcct tcaccctggt tgatcacgcg
    49501 gaattgcagg ccagattcgg tgctattcac accttctttt ttggcgtttt cttccaggta
    49561 tttcacacct tcagcagcca tcgcctggaa acgctgacga cgaacggcat cggcgcgctc
    49621 gtggatttca cgcagcgcgc gatgcaccac atcaaccgga acagccggat gtttgccttc
    49681 cagcgcatcg gcaatacctg caaccagtgc ttctggcagc agcccttcca ggccagattc
    49741 actcagttgt tgccctacct gcaaaccaat gccgtagctt gcttgcgctt cgatggtgtc
    49801 aaaagttggg gtggtcatcg tatttccttt cttagggtat aaaagtagcg ggcagcatat
    49861 cagccacact tctttgggta aaactttgtc gcaggaatga tgacaaatcc cggggaaaaa
    49921 gaaacaatag caatatcccg tatgaatata ataaaggcgg gtttaccacg gacatcatag
    49981 cgttagctta ttcataggct atgattgagg aacaagacgc ggagcaggag gaaaccatgc
    50041 ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg gcacgcgccg gacaattttc
    50101 gcttcatgga cccgctgccg ccgatgcatc gtcgggggat cattattgcc gccattatat
    50161 tggtggtcgg atttctgctt ccatctgatg atacgcccaa cgcgccggtg gtgacgcgtg
    50221 aagcgcagtt agatattcag tcgcaatcac aaccgccgac agaagaacag ctgcgggcgc
    50281 aattagtcac accacaaaat gatcctgatc aggtggctcc ggtcgctcca gaacctatac
    50341 aggaaagcca gccagaagaa cagccgcaaa ctacccaaac acaaccattc cagccagata
    50401 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    50461 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    50521 cgggtaagcc gctgagtaat ttgcaaaacg ggcaaatggt gaaaatccgc cagaacgcca
    50581 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgttcaccc
    50641 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    50701 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    50761 gatatgataa accaggccaa gcgtatgctg gttgatcaaa taatatccgc caagcgcagc
    50821 aatattaata ttcacctgca atgggtcgat atctggctta aaaatatttt gtttcttccc
    50881 ttcatccaat aaggattcca ttaactgcaa gtgcgcatgg ttaatttcta aaagacgctg
    50941 tgattttgca taatgcacac ccttactttg attttcacta tgcacaattt ttaaaaacca
    51001 gggattagaa atataataat cccatgtaaa atcgattatt ttctcaatgg ctttctgtgg
    51061 cggaagatct gaga
//
LOCUS       DAOFSV010000017        50488 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.017, whole genome shotgun sequence.
ACCESSION   DAOFSV010000017 DAOFSV010000000
VERSION     DAOFSV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.017"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(57..1730)
                     /gene="rpsA"
                     /locus_tag="QBH27_001096"
     CDS             complement(57..1730)
                     /gene="rpsA"
                     /locus_tag="QBH27_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDQ7013435.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(1841..2524)
                     /gene="cmk"
                     /locus_tag="QBH27_001097"
     CDS             complement(1841..2524)
                     /gene="cmk"
                     /locus_tag="QBH27_001097"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDQ7013436.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(2697..3479)
                     /gene="ycaL"
                     /locus_tag="QBH27_001098"
     CDS             complement(2697..3479)
                     /gene="ycaL"
                     /locus_tag="QBH27_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDQ7013437.1"
                     /translation="MYLKSNQKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNA
                     YKAATLSDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYK
                     VYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAI
                     VAARDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQ
                     GLVGSFEKLASLDGGHTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(3623..4012)
                     /locus_tag="QBH27_001099"
     CDS             complement(3623..4012)
                     /locus_tag="QBH27_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ7013438.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPEVADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNHHELARKFGVSLQWVYSVVK
                     RVRKEELDRMQGKLFADEPDADTEKKE"
     gene            complement(3984..4433)
                     /locus_tag="QBH27_001100"
     CDS             complement(3984..4433)
                     /locus_tag="QBH27_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDQ7013439.1"
                     /translation="MQKKRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKKKGFRVKPARKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLSTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            complement(4435..4641)
                     /locus_tag="QBH27_001101"
     CDS             complement(4435..4641)
                     /locus_tag="QBH27_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013440.1"
                     /translation="MTTETIVCFLFWYMYAGSCSARLHRSLGYGRHYDTAHYILYMTA
                     VMLFWPVTLPAATDIVADRLKKRR"
     gene            complement(4631..4861)
                     /locus_tag="QBH27_001102"
     CDS             complement(4631..4861)
                     /locus_tag="QBH27_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013441.1"
                     /translation="MITLSGKSRKLKACRISARYLFARAFFKNVRPGITIGVIAGREQ
                     VEKYMSGAWWNNDPVIAARNIHIEWGGIQNDY"
     gene            complement(4858..5541)
                     /locus_tag="QBH27_001103"
     CDS             complement(4858..5541)
                     /locus_tag="QBH27_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDQ7013442.1"
                     /translation="MTDQNKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREVFGVNSIIHPGTHPSVGFYGNR
                     ERAALAAYTWEVLARQLKKARQQYISAQNKRIKTATRTSRGDQFAEGWVLAVISEIQS
                     FALTDDERELMQQWLEHKYPQTQTTRARKPGRSRNGDASRYAGFREGQNVRLHRPVSG
                     QEQQKLEAR"
     gene            complement(5538..5753)
                     /locus_tag="QBH27_001104"
     CDS             complement(5538..5753)
                     /locus_tag="QBH27_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013443.1"
                     /translation="MADILREITAWTLILTGLATCLSAGAALAALLMHITTQWLWEKL
                     KAAYSLKELSDAVRAWKRQKNTGDTEQ"
     gene            complement(5768..6064)
                     /locus_tag="QBH27_001105"
     CDS             complement(5768..6064)
                     /locus_tag="QBH27_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013444.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLAEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVIYTNE"
     gene            complement(6074..6346)
                     /locus_tag="QBH27_001106"
     CDS             complement(6074..6346)
                     /locus_tag="QBH27_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013445.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTDVKHVLTALISMATQA
                     IVATNGKEAALQVLMNTLTHTAEHEVPYRMETTAEGGLHITVSRKH"
     gene            complement(6635..7165)
                     /locus_tag="QBH27_001107"
     CDS             complement(6635..7165)
                     /locus_tag="QBH27_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HDQ7013446.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGE
                     VQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
                     SDIEDFSIIPFEQDVQ"
     gene            complement(7193..7462)
                     /locus_tag="QBH27_001108"
     CDS             complement(7193..7462)
                     /locus_tag="QBH27_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013447.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSITIRGNKPVIRVSR
                     SAHCMRMLEQGKACYLYTGHDHRGYFRQGVFELHGCRVVWPESLW"
     gene            complement(7465..8631)
                     /locus_tag="QBH27_001109"
     CDS             complement(7465..8631)
                     /locus_tag="QBH27_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ7013448.1"
                     /translation="MLVLKQQLKEARIPQAVVARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTTKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSDDVFTTPDIRYVREALYQTARHGGFMAVIGESGAGKSTL
                     RRDLTERINRENAPVIVIEPYIIAMEDNDVKGKTLKAAAIAEAIISTIAPLESIRRSQ
                     DARFRQLHRVLKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLSIVLI
                     GQPELATKLSERNMEVREVVQRCEVVELLPLDNNLEEFLTFKLQRAGKQLTDIMDAGA
                     VDAIRARLSNPGSHRKNMVSLLYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
     gene            complement(8642..10411)
                     /locus_tag="QBH27_001110"
     CDS             complement(8642..10411)
                     /locus_tag="QBH27_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDQ7013449.1"
                     /translation="MTPALTEKLVETARAARDAGHGKRGAIYDAACAELGMSRATLLR
                     RLKEVSVTDKRKKRADAGRSALTRDEAALISATLREATRKNGKRLYSIADAVETLRAN
                     GFITAGRTDETTSEFFPLSEDAISRALRNYGLHPEQLDAPAPHTEVASLHPNHVWQID
                     ASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARIASDRVWSYEITDHASGWIYVEYV
                     TGAESGENLCSVLINAMQERGGADVLHGVPKILYLDPGSANTAGMTKNMCRSLGIDLI
                     AHKPHNARATGQVEKARDIIERKLEPGLKFRPVHSLEELNALAAKWRSHFNATAVHSR
                     HGKTRTDIWLKITAEQLKKAPSVEVCRELAVAAPELRKVTPKLRVSFRGTEFDVSTVP
                     GVLVGEKLMITRNPWRSDVAQVVLTGEDGHETFFLVEEVRKNEFGFAEGAAVFGESYK
                     ALPETPAQMAAKETEALVTGTDNAADAAAARKAKALPFGGRLDPYKHIDDTTLPAYMP
                     KRGQASDVRGPRTEQRPMTHVEAAKALRDKFSADGLTWTPEHYRQLTAQYPDGVPEAA
                     LDEVMATLTTPARSSVISIVNGN"
     gene            complement(10427..10744)
                     /locus_tag="QBH27_001111"
     CDS             complement(10427..10744)
                     /locus_tag="QBH27_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013450.1"
                     /translation="MNTNNTQNTLMDSPEALGHALCNLLPEMVQGFRVVTPSGEICVP
                     AQETHPFVLTMEVMLMQQIRRLQNQSALRPVVAPQPVNTVVKTCDGETLCDLARKIAA
                     RIG"
     gene            complement(10744..11664)
                     /locus_tag="QBH27_001112"
     CDS             complement(10744..11664)
                     /locus_tag="QBH27_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDQ7013451.1"
                     /translation="MGRTKSPINTELNADVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERERIVHEARFYMAQSAESMLEAGKRLIILKENEPHGDFTNILENDLGLAPQVARR
                     MMQASVKFLGNGDEQPKRSALSVLGKTKLYELMVLDDEELDALADGGTVAGATLDDID
                     RMTSRELKAALREARETNAAQQQVLAGKDEKINELATKLEKKSRLQPPPPDEELKKLR
                     AEVTALAVEAESAIAVRLSSVFETLCEYCTKNMIDTPRDFMAGLVCQIERSVHALRET
                     FDLEAAPSGNDAPAWLTEPEPEINRPEDMQ"
     gene            complement(11675..11983)
                     /locus_tag="QBH27_001113"
     CDS             complement(11675..11983)
                     /locus_tag="QBH27_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7013452.1"
                     /translation="MSKTNVSSSGSRILRVLKALRGHALNGISNGELATALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            complement(12036..12224)
                     /locus_tag="QBH27_001114"
     CDS             complement(12036..12224)
                     /locus_tag="QBH27_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ7013453.1"
                     /translation="MTAEQVKALFRQRGITFTRWAEENGYNRNEVYRVLNGQTKARYG
                     KSHEIAVKLGLKSTAQAA"
     gene            12318..12674
                     /locus_tag="QBH27_001115"
     CDS             12318..12674
                     /locus_tag="QBH27_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7013454.1"
                     /translation="MSKDIGERVISVRKKAGLNQREFAARLGISNGGISQIENGKAMP
                     GGEFLLKMHQEFNVDVNWLLTGMSTDGMTRNQNQISPEKQKLIDTFDDMTPDQQRSFL
                     EIGRFYTQPKPGKIAG"
     gene            complement(12791..13555)
                     /locus_tag="QBH27_001116"
     CDS             complement(12791..13555)
                     /locus_tag="QBH27_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDQ7013455.1"
                     /translation="MKMQTLPIVPWIGGKRRLAKHILPLFPAHECYVEPFCGAAALYF
                     LKTPGKIEVINDINGELVNLYRVVKHHLEEFVRQFKWALVSRQIYKWLQITPEETLTD
                     IQRAARFYYLQKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTVIEHM
                     DWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIISVNDI
                     PQMREVFTGLNIQTVNINYSLAGKPTPRRELLICNF"
     gene            complement(13746..13961)
                     /locus_tag="QBH27_001117"
     CDS             complement(13746..13961)
                     /locus_tag="QBH27_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013456.1"
                     /translation="MNPRLTLTEHQRRAEAVNNVLEDIIRLYRGELSVCRAAFHFQGI
                     QKQFDTSVFAEGITYALDRIRSENRPG"
     gene            13963..14364
                     /locus_tag="QBH27_001118"
     CDS             13963..14364
                     /locus_tag="QBH27_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013457.1"
                     /translation="MHSLNDDFYVLTGKIFCRLLNTHPAPQVLIYSGFFDIPDQVDHD
                     QKKCRCFIDTLIWLRDNSYIRYSQLLAVGAADVVLTEKTLCLLNAMPTFLQPRPVPFK
                     ESLLDAVKTGENAVIAECVSRIMRYVFNERK"
     gene            complement(14340..15068)
                     /locus_tag="QBH27_001119"
     CDS             complement(14340..15068)
                     /locus_tag="QBH27_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013458.1"
                     /translation="MTQTLNPVITVTVSGPVGSGKSYVLARIEEVVKQEFGNSVIVDA
                     ADVDGERRMSGDDLSTWQKPRAGTVIRLEEYTGQGLLSYGEERICRTNELLDVICPTG
                     RQPWILPDALHATIAALMTVYDQELLMKELRKLTGQSTECCSSPKYGKQEHIPINQPP
                     RFFDEYLLTPENKAEHEEIKAALFREIRKIAEMQLEGGLDRVMARGEWASLFERVSPE
                     LLPLALAIGIMQTADKPHLRSLNT"
     gene            15199..15549
                     /locus_tag="QBH27_001120"
     CDS             15199..15549
                     /locus_tag="QBH27_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HDQ7013459.1"
                     /translation="MKNLKKFIPPVKKPRLSGWLLTSVLLLGTIALVSPQQLPVVIYK
                     LALITLAAVLGYWLDRSLFPKARPGQYLKHDDRLMAEGRFPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVAMGL"
     gene            15552..16292
                     /locus_tag="QBH27_001121"
     CDS             15552..16292
                     /locus_tag="QBH27_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDQ7013460.1"
                     /translation="MNWPQITWIVCMSLKLAFEAFRVFIRTERLSVRAGTFLVHMAWV
                     FFVAMLLWCGGFFSQACAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESG
                     WRPDAISPAGAQGLAQFMPATADWISQLIPMLSSREPFNPAWAIRALVSYDRWLWQRV
                     SAASDCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRE
                     NRHYPQRILRELAPRYLTWGGSSCVASG"
     gene            16276..16926
                     /locus_tag="QBH27_001122"
     CDS             16276..16926
                     /locus_tag="QBH27_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013461.1"
                     /translation="MWHLVKKLPWRGILLAILINAFLVGLYAMGYRSGHDSAKRDGDT
                     ALSQLQSAFDAYKTEQATLENAALRAWARRYQEQVAAGQRAEAGYLEQIAQLESRNKQ
                     LQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAATGT
                     RSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAWQCRRWRNQINALLDEREGLQK"
     gene            16923..17249
                     /locus_tag="QBH27_001123"
     CDS             16923..17249
                     /locus_tag="QBH27_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013462.1"
                     /translation="MTLQVEFWTVVSFLLTFMRFVGGLAKWLFSKTEERQAARFASLE
                     QALQQSASNWGELEKEFMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            17249..17560
                     /locus_tag="QBH27_001124"
     CDS             17249..17560
                     /locus_tag="QBH27_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013463.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLDVSRAIYPDT
                     TPLELRRELDYLADRKMVELEKKPSGDWFADLTRLGVDLVEYTVECGPGIARPEKYWS
                     E"
     gene            17560..18105
                     /locus_tag="QBH27_001125"
     CDS             17560..18105
                     /locus_tag="QBH27_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDQ7013464.1"
                     /translation="MARRSTIEKLPEDVRRWLERALTESGFSGYNELESLLRERGYVI
                     SKSAIHRYGQKIERRYGAIRAATEAARMLTEGAADDQDARSEAVIALIQTELFESIVQ
                     LQEAEEGEVDPKERVALLSKVAKNVATLSRASVNLKKFQSEVRARAQQAASNAEKIAR
                     KGGLSSDAVQALRREILGIAT"
     gene            18156..19697
                     /locus_tag="QBH27_001126"
     CDS             18156..19697
                     /locus_tag="QBH27_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013465.1"
                     /translation="MSPMDGRNNLAPVLPDTSALDAPPVLLPYQQRWVADTSPLKVIE
                     KSRRTGITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAA
                     SEIEEGFWEEDDDDKHIKTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQ
                     LDELLKAALAMLIWGGKVRVISTHDGDDNPFNTLIGDIRAGRQGGSIHRITFREAVSE
                     GLFRRVCLRTGKEWSEASEQAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESR
                     MSADTPVLRLTCKEGYELLSDEVRFRETQDWLDEYLKPLLEALPTDARSFLGRDFGRS
                     GDLSVDYPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNG
                     AYLAEYAMQRYGSGRVQQVMPTEGWYREHMPPVKAALEDGNLVDLPKDEDTLDDLRAV
                     QVVNGVPRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAAR
                     MLEGF"
     gene            19697..21193
                     /locus_tag="QBH27_001127"
     CDS             19697..21193
                     /locus_tag="QBH27_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDQ7013466.1"
                     /translation="MARGIWVSPDEFVAFSEPQKSLTAQIASRSRAIDFYGLGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAALKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRSWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVAAIPDDSSIELRAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQEAIDDTRATRDEKLTRAGLRLTPQYFKREYQLQDGDID
                     ETPPSERQNNMLPLSFAEAIDADIQAQQQLDDALDILMNGGALNGTLEPVLAPLFRRV
                     ENGVNPSELLGELAELYPQMNTDDLQERLARILFVANIRGRLHERDNG"
     gene            21174..21995
                     /locus_tag="QBH27_001128"
     CDS             21174..21995
                     /locus_tag="QBH27_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDQ7013467.1"
                     /translation="MSVTTAELAYCMTLPPKRAINYLKSKGYQITWDWEEMWQEAHAR
                     AFTVAKVTRLDILEDIRGALQQAVDEGKTDRWFRQELEPVLKRKGWWGPRDTTDPVTG
                     EPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSAMGQELKLV
                     SEKTGEMRNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            21998..22456
                     /locus_tag="QBH27_001129"
     CDS             21998..22456
                     /locus_tag="QBH27_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDQ7013468.1"
                     /translation="MASDNLVSITINDKSLRRSLRALDLAATDLEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWMPSLAAEERDGQTLQDTGRLMGSVSTDHDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTGDGELQPEVVIPILDTIVRHLESAARR"
     gene            22671..23786
                     /locus_tag="QBH27_001130"
     CDS             22671..23786
                     /locus_tag="QBH27_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDQ7013469.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTLSQDAAGAQFSETGPGPSSASNEESSMT
                     KEEIEALQEENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VAEFAEADPERLALHQKAVALSKKEGISYEAAVARCL"
     gene            23801..24754
                     /locus_tag="QBH27_001131"
     CDS             23801..24754
                     /locus_tag="QBH27_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013470.1"
                     /translation="MSDYLKGKRVVDPVLTSIARGYKNAAFIGERIFPVVLTDKEGVR
                     VPTFGKTAFVEYDTERAVGADSNVLVREKTGTLDLVLGEHDLAAPVDYREQAESMFNE
                     ESKAIRRATNGVNLRRELIAARLAQDEKVYRTGHVKKLTASDRWAGGKGDPIGVIEAG
                     MEAVRTATGLRPNLMTMGAGVMALLKFHPAIQAAIGANERKRITTEILQDLFQIEEIV
                     IGAPVSLPSMKAAMDKNSVPADIWGDNLMLHYVGKPQPGADSADENEPSFGYTLRRKG
                     MPVADKYDGAGGKVKYCRYTDIYKVAVVGGDAGYLITGISK"
     gene            24764..25102
                     /locus_tag="QBH27_001132"
     CDS             24764..25102
                     /locus_tag="QBH27_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDQ7013471.1"
                     /translation="MGTTQQVILITTVTAGAALAQQRFVGADNTPCKAGAAALGVAEV
                     DAVTGDSTPVSVLGIIAVEAGAAVSRGVAVQSDAQARAVPQSGDGKSCGIALDEAGGE
                     GDVIRILRGV"
     gene            25104..25550
                     /locus_tag="QBH27_001133"
     CDS             25104..25550
                     /locus_tag="QBH27_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDQ7013472.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEEMDFDSPATVNTRVVDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            25550..26014
                     /locus_tag="QBH27_001134"
     CDS             25550..26014
                     /locus_tag="QBH27_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDQ7013473.1"
                     /translation="MNVLPVLDAVLARLREKLPQLQVEYFPEKPAEYRLNHPVGALLL
                     SYAGSRFDRPDDTGAVIQSQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGHTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            26014..26265
                     /locus_tag="QBH27_001135"
     CDS             26014..26265
                     /locus_tag="QBH27_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013474.1"
                     /translation="MKEYRYSGPASGVTLSDGTEILLWPGKTVSLPEEHDYVKVLVAL
                     KHLTPVPEETKPAGTPAVQSPKRRSSSDSEVKTEDTHGS"
     gene            26255..27682
                     /locus_tag="QBH27_001136"
     CDS             26255..27682
                     /locus_tag="QBH27_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDQ7013475.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIEDEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTTHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMAEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            27679..28203
                     /locus_tag="QBH27_001137"
     CDS             27679..28203
                     /locus_tag="QBH27_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDQ7013476.1"
                     /translation="MMSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKAL
                     GMVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYRSLALQCRSSVQRYSSQGLIDE
                     IPLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRVG
                     GVDQLTDYRINIGG"
     gene            28206..28487
                     /locus_tag="QBH27_001138"
     CDS             28206..28487
                     /locus_tag="QBH27_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013477.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARKLADLIEAWEPPDHWFSGIGKSEGKTLLIAFLRNCKGFRTC"
     gene            28585..28920
                     /locus_tag="QBH27_001139"
     CDS             28585..28920
                     /locus_tag="QBH27_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDQ7013478.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESNQGADAGAGAAGE
                     MVPVSAGGD"
     gene            28844..29002
                     /locus_tag="QBH27_001140"
     CDS             28844..29002
                     /locus_tag="QBH27_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDQ7013479.1"
                     /translation="MRATRALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            29078..32029
                     /locus_tag="QBH27_001141"
     CDS             29078..32029
                     /locus_tag="QBH27_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ7013480.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     LKYNQYQDELQEAMGSLISDNIDNVSDIGFLMPDIARAATATRTSAQDWAKVAAVWQN
                     SLKGAARDFGAVQNIMAYAGDQGSFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSIASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKTLMVSSRDLAITLGDQL
                     APSFISLTQDLLPLIQGTKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVNVWKTAVLLRTNWHRLTTALGEGGKLRWLVTGFSRLTSGGLKLSKVLAGSLVRGF
                     MSAARAVLWIGRALMMNPIGLVITAVAAAAYLIYRNWGAVSGWFKQRWADIQEAFNGG
                     IAGTGKLLINWSPAGLLYKAFAAALKYFGVTLPAKFTDFGGHLIDGLINGIKNKWGSL
                     KSSVTGMGDSISSWFKQCWADIQEAFNGGIAGTGKLLINWSPAGLLYKAFAAALKYLG
                     VDLPAKFTDFGGHLVDGLINGIKNKWESLKSSVTGMGDSISGWFSEKLGIHSPSRVFM
                     GFGDNIAQGAAIGLQRTTPLAALAGQRMASELLPRMPVSIQGPEIRDNTSGVRFSMPL
                     PDINGFMSSAKNAIGAVINSLSSMPAIPLSTRASILPGQRLANEMTPDVPRIPSPEIL
                     AAGYSGRGAAATGGGTSGGIQVSFNPQFFLNGRETTAPAGLTGALNMSLHELEKMLER
                     LLAQKQRKEYR"
     gene            32029..32913
                     /locus_tag="QBH27_001142"
     CDS             32029..32913
                     /locus_tag="QBH27_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7013481.1"
                     /translation="MFAVLGDIEFELITYWDGFEATFGVDYAEHARIGGKPGLQFVGD
                     RLDEIQITLVFHQHYCVPDVELARLRTAMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRTKLPGVGAVSGAIPSPSGVAQFIR
                     NGVNYAKQAQSVLQTTISAVRVTQKMKDNPVVALTRVPGLMSGLGNISGALGKSVPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAVGTAFN
                     RMSPGLSAMAARILTRSV"
     gene            32913..33125
                     /locus_tag="QBH27_001143"
     CDS             32913..33125
                     /locus_tag="QBH27_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDQ7013482.1"
                     /translation="MFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVLP
                     SGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            33113..34267
                     /locus_tag="QBH27_001144"
     CDS             33113..34267
                     /locus_tag="QBH27_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDQ7013483.1"
                     /translation="MAEINSTAQVTSALTGVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVSFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            34264..34860
                     /locus_tag="QBH27_001145"
     CDS             34264..34860
                     /locus_tag="QBH27_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDQ7013484.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLIIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGEGVTATIHGNIEIKGNVHATGSMLSDGENSSHHSH"
     gene            34915..35262
                     /locus_tag="QBH27_001146"
     CDS             34915..35262
                     /locus_tag="QBH27_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDQ7013485.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            35253..36356
                     /locus_tag="QBH27_001147"
     CDS             35253..36356
                     /locus_tag="QBH27_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDQ7013486.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFAREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     AIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTATCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVNLLVSNLMASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMTGVSDD"
     gene            36349..36927
                     /locus_tag="QBH27_001148"
     CDS             36349..36927
                     /locus_tag="QBH27_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDQ7013487.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
     gene            36930..38210
                     /locus_tag="QBH27_001149"
     CDS             36930..38210
                     /locus_tag="QBH27_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDQ7013488.1"
                     /translation="MGNLNETEKWEENIYQLETSDPVLGGADGISNRAPRQLANRTKW
                     LKKKTEEAAQSLAEHVRSRNHPDATLTAKGFTQLSSATNSTSETLAATPKAVKAAYDL
                     AAGKAPASHTHPWSQITGVPAASLTAKGTVQLSSATDSQSETEAATPKAVKAAYDLAA
                     GKAPVSHTHPWSQITGVPAASLTAKGTVQLSSAINSTSEILAATPKAVKAAYDLANGK
                     QPADATLTALAGLATAADRLPYFTGADRAELATLTAIGRAIIAKGSIKDVLNYLGLGE
                     GSALPVGVPVPWPTATPPAGWLQCNGATFTKEQYPVLVRVYPTLRLPDLRGEFIRGWD
                     GGRKVDTGRALLSFQEGTIVSGFDDNDTGDISSLGSTQYGFGDALTSNQLGVLNGKKW
                     IFDASSKGAQRYDWWAYVSARPRNIAFNYIVRAA"
     gene            complement(38217..38831)
                     /locus_tag="QBH27_001150"
     CDS             complement(38217..38831)
                     /locus_tag="QBH27_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ7013489.1"
                     /translation="MSNTAVLDENGIATVAGDITVYHYDEETREYTSSSVEYLALGVG
                     TPAHSCADAPPEAISGYVVCRTATLNGWEHVPDHRGETVYSTENGNPVLITQPGDYPA
                     DTTTKQPATPWDTWNGEAWVTDTERQRAAELEVARQQRQQRVKQAMASVDLINLKLRA
                     GRSLKPEETAKLNAVLDYIDELNALDISKAPEISWPEAPLALAG"
     gene            complement(38831..39313)
                     /locus_tag="QBH27_001151"
     CDS             complement(38831..39313)
                     /locus_tag="QBH27_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDQ7013490.1"
                     /translation="MPVPWPTATPPAGWLKCDGRAFTKEQYPVLARVYPTLRLPDLRG
                     EFIRGWDDGRKVDTGRKLLSAQGATLLRTAMLDYYNQDTTGTSGIVGMGFNNEDSITD
                     LREGSFKMPDGTTFSDPVVAMSDNGMQATILTSIRSGYAKGITVRPRSIALNYIVRAV
                     "
     gene            complement(39354..39794)
                     /locus_tag="QBH27_001152"
     CDS             complement(39354..39794)
                     /locus_tag="QBH27_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDQ7013491.1"
                     /translation="MNVGLGEGSALPVGVPVPWPTATPPAGWLKCDGRAFTKEQYPVL
                     ARVYPTLRLPDLRGEFIRGWDDGRKVDTGRDLLSRQDGTSFSHYAGNFDIGSGHSINN
                     YDQIVDNQPGFSRFSFAGPSRGDGVNYVTIRPRNIAFNYIVRAA"
     gene            39854..40426
                     /locus_tag="QBH27_001153"
     CDS             39854..40426
                     /locus_tag="QBH27_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ7013492.1"
                     /translation="MLIGYIRVSTNDQNTDLQRNALMCAGCEQIFEDKMSGTKSERPG
                     LKRALKCLKRGDTLVVWKLDRLGRRMKHLIALTEELRAKGVNFRSLTDSIDTSTPMGR
                     FFFHMMGALAEMERELIVERTLAGLAAARAQGRVGGRRPKLTKEQHEQIARLLQKGYD
                     RKRLAIIYDIGLSTIYRYHPVGTVITQPEM"
     gene            complement(40682..41965)
                     /gene="aroA"
                     /locus_tag="QBH27_001154"
     CDS             complement(40682..41965)
                     /gene="aroA"
                     /locus_tag="QBH27_001154"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDQ7013493.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLSGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(42036..43124)
                     /gene="serC"
                     /locus_tag="QBH27_001155"
     CDS             complement(42036..43124)
                     /gene="serC"
                     /locus_tag="QBH27_001155"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDQ7013494.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(43323..44015)
                     /locus_tag="QBH27_001156"
     CDS             complement(43323..44015)
                     /locus_tag="QBH27_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDQ7013495.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            44145..45905
                     /locus_tag="QBH27_001157"
     CDS             44145..45905
                     /locus_tag="QBH27_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDQ7013496.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYSCRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            46311..47168
                     /gene="focA"
                     /locus_tag="QBH27_001158"
     CDS             46311..47168
                     /gene="focA"
                     /locus_tag="QBH27_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDQ7013497.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            47223..49505
                     /gene="pflB"
                     /locus_tag="QBH27_001159"
     CDS             47223..49505
                     /gene="pflB"
                     /locus_tag="QBH27_001159"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDQ7013498.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            49697..50437
                     /gene="pflA"
                     /locus_tag="QBH27_001160"
     CDS             49697..50437
                     /gene="pflA"
                     /locus_tag="QBH27_001160"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDQ7013499.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT        11575 a        13336 c        13588 g        11989 t
ORIGIN      
        1 aatctgtcaa gtaaactcaa caaacttcgg aataaaaatc ccgaagagtc agagaattac
       61 tcgcctttag ctgctttgaa agcttcagcc attgcgttgt tggagaagtt tgcatcttcc
      121 tgtttgttaa cagttgcgat tgcatctttc tcgtcagctt cgtctttcgc acgaacagac
      181 aggctgattg cgcggttttt acgatcaacg ccggtgaatt tagcttcaac ttcgtcgcca
      241 acgctcagaa ccagggtagc gtcttcaacg cggtcacggg atgcttcaga agcacgcagg
      301 taaccttcaa cgccgtcagc cagttctacg gttgcgcctt tagcgtcaac tgcagttact
      361 ttaccggtta cgatagcgcc tttcttgttc agagcaaccc agttgttgaa cggatcttct
      421 gcgagctgtt taacgcccag ggagatacgt tcacgttctg cgtcaacctg cagaacaact
      481 gcagcgattt cgtcgccttt tttgtattca cgaactgctt cttcgcctgc aacgttccag
      541 gagatgtcag acaggtgaac caggccgtcg atgccgccgt ccaggccgat gaagataccg
      601 aagtcagtga tagacttgat tttaccttca acacggtcgc ccttgttgtg ggtttccgcg
      661 aactgctgcc acgggttagc tttgcactgt ttcagaccca gggagatacg acgacgttct
      721 tcgtcgatat ccagaaccat aacttccact acatcgccaa cgttaacaac tttggacggg
      781 tggatgtttt tgttggtcca atccatttcg gaaacgtgta ccaggccttc aacgccttct
      841 tcgatttcaa cgaagcagcc gtagtcggtc aggttggtca cgcgaccagt cagtttggta
      901 ccttccggat aacgtttagc gatagctacc cacggatctt cgcccagctg tttcaggccc
      961 agggatacac gggtacgttc gcggtcgaac ttcagcactt taacagtgat ttcgtcgccc
     1021 acgttgacga tttcgctcgg atgcttaacg cgtttccagg ccatgtcagt gatgtgcagc
     1081 aggccgtcaa cgccgcccag atcaacgaat gcaccgtagt cagtgaggtt cttaacgata
     1141 cctttaactt ccatgccttc ctgcaggttt tccagcagct gatcgcgctc tgcgctgttt
     1201 tcggattcga taacggcacg acgagaaaca acaacgttgt tgcgcttctg atccagcttg
     1261 attactttga attcaagctc tttgccttcc aggtgcagag tgtcacgcac cggacgaaca
     1321 tctaccagag aacctggcag gaacgcacga ataccgttca gctcaacagt gaagccgccc
     1381 ttaactttgc cgttgataac accggtaaca gtttcagcat cttcgtaagc tttttccagc
     1441 gtgatccagg cttcgtgacg tttagctttc tcacgggaca gcagagtttc accgaagccg
     1501 tcttctactg cgtccagagc aacgtcaact tcgtcaccta cctggatttc cagctcgccc
     1561 tgggcgtttt tgaactgctc agccgggatg gcggactcag atttcagacc agcgtcaacc
     1621 agtactacgt ctttgtcgat agcaacaaca acgccacgaa cgatagaacc cgggcgggtt
     1681 tcgatttctt ttaaggactc ttcaaagagt tgagcaaaag attcagtcat gtttaatctt
     1741 caggtttata tttaacgtcc acctggctcc atgccggatg gggttgttta acatacccgc
     1801 tgtcattcca ttgcaacggg ggtactgcaa attcggtcgc ttatgcgaga gccaatttct
     1861 ggcgcgcgta ttgtagcgct ttttcaatca cttgctcaat gcttaaggtg gtggaatcca
     1921 acactaaagc atcggctgcc ggaaccagtg gcgctaccgc tcgattacga tcgcggtcgt
     1981 cacgttcttt gatctcggcc aaaaggcgct caaagttaac actaaagccc ttctcctgca
     2041 actgtagcat gcggcgatgc gcacgttctt ccgaggaggc gtcaaggaaa attttcactg
     2101 gcgcatcagg gaataccacc gttcccatgt cgcgcccatc ggcaatcagg cctggtaatt
     2161 cgcgaaacgc gcgttggcga cgcaataatg cttcacgaac gcgtgggaat gccgcgactt
     2221 gtgaagcagc attcgccacc tcctgagtac gaatttcgcc gctgacatct tccccttcga
     2281 ggatcacttc cagattgccg ttggttgaca caaaacgtac atccagatgg gatgccagcg
     2341 gcaccagcgc atcttccgat gcaacatcaa catggtgatg taatgccgct agtgccagta
     2401 cgcgataaat tgcacccgaa tccagcagat gccattgcaa cgcttccgcc atagccttac
     2461 acaaggtgcc tttccctgca ccgcttgggc catcaatggt aataaccggg gcaattgccg
     2521 tcatctttat ctccttaaaa caggcgtacc gttaacataa ccgcgcgcat tatacgcgcc
     2581 gacgccacaa atcgttaacg ttgtgtgcaa aaaccagttt tgtctgtatt cagatgggga
     2641 tttattgcgc aattatagcg ggctggcaga agaccagccc gaaagatgac aatgatttac
     2701 ttaccagagg cgatacgatc acggatgtgt tgcgcacgct ctgttgatgg tgggtgagaa
     2761 tcaaacatgg actgggtgtg accgccatcc aggctagcga gtttttcaaa gctaccaacc
     2821 agcccctggg tactgatgcc acgtttcttc aacagatcaa aggagaaatc atctgcttct
     2881 gactctttat cacgggagta tttagcattg atagcgcctt ctgcgatatc acccaattgt
     2941 gagcgggaaa gctgggaagc cacaccgctg gtagctgaaa tggcatcgcg tgcggccacg
     3001 atcgcataag aagctttcat ttcagccagc gagtgaccca acgcgacgtg gcccagttca
     3061 tggcccagaa cgccttcaat ttcgttgtcg ttcatcatgt ccatcaggcc actgtagaca
     3121 cgaacacaac cgttcgccat cgcccatgcg ttgacgtcgc tggtcatata aaccttatag
     3181 ttgactggcg tgccattaat attgttaccc aatgctttgg cgatttttgc cagacgtttc
     3241 ccgtacttgc tggatttgct tgccacttta ttgccgctgt ccatttcgac acagccctga
     3301 ttggcgattg ctttagcatc agcatcgctt aatgttgcgg ctttataagc attcagacct
     3361 gagctgatag ccaaatcggt attaataccg tgcgtattct gacatcctgt cagtaaggct
     3421 gcagaggtcg caatcgccag caataattta gtattcttct gattagactt gagataaatg
     3481 acagggaata agtgagatat ttatgggaag aactgggtca aaatgggaag agactaggag
     3541 gaatatgcaa taaccttaca gtatgtgaaa atcagaccgg cccgaaaaaa aaacagaact
     3601 cgcgccagcc tgattgcaga tattactctt ttttctccgt atctgcatcg ggttcatcag
     3661 caaatagctt gccctgcatc cgatccagtt cttcttttct tacccgctta accacgctgt
     3721 agacccactg aagcgaaaca ccaaatttgc gggccagttc gtggtggttg cgtccgttaa
     3781 actccctgaa gatttcccgg tcgcgctgac tgaccttcca taccatgccc atcgggaaat
     3841 aaacgttttg cccgccccag acctgcatca ttcggttcgc gacggcctga ccaatctggt
     3901 cggcaacttc gggctcaata tcaataatct cgcgaacggt ctcagaggta tgctgtgcca
     3961 gttccaccag gagttccggc cccttacttc gaaactgatt caggtcgctc atgtttgact
     4021 cccgcagctc tgcgctgcca cttcttcagt ttctcaataa cactgcttgc ctgttcagta
     4081 ctgagccagc gcagggcgct gatgcccgtt tcccgcttga tccacctcgc taatgcattt
     4141 tctgaacggt cacgaacaat gccggcagca gccatttcaa gccatagcgc acgaattttc
     4201 ctggactgcg gatggttatc aagcggtaaa ccggagctgg cttttctggc tggtttaacg
     4261 cgaaagcctt tctttttcat ggattccagc acgcagttta gttgtgtggt atccattcct
     4321 ttggttgagg ctttaccggt cagcccctgt aacatctggc ggtaggtgtc ttcatccata
     4381 cccagttcat tacgggcaat atggataagc tgtatcaggc gttttttctg catatcatct
     4441 cctttttttc agtctgtcgg caacaatgtc ggttgcagct ggcagggtga ctggccagaa
     4501 caacatgacg gcggtcatgt acaggatgta atgcgcggtg tcgtagtgcc tgccatagcc
     4561 cagggaacga tgaagtcttg cgctgcatga tcctgcgtac atataccaga aaagaaaaca
     4621 gacaatggtt tcagtagtca ttctgaatac ctccccattc gatatgaata ttacgggcag
     4681 caatgacagg gtcgttattc caccatgcac ctgacatgta tttttcaacc tgttcgcgtc
     4741 cggcaataac accaattgtg atccccggcc tgacgttctt aaaaaaggcg cgggcaaaaa
     4801 ggtatctggc agatattcgg caggctttta atttccggct tttacctgat agcgtaatca
     4861 tctggcctcc agtttctgtt gttcctgccc actgaccggg cggtgcagtc tgacgttctg
     4921 cccttcacga aaccccgcat agcgcgaggc gtcgccattg cggcttcttc ccggtttacg
     4981 cgccctggtg gtttgcgtct gcgggtattt atgttccagc cactgctgca tcagttcacg
     5041 ctcatcatcg gtcagggcaa aggactgtat ttcactgata acggccagca cccagccttc
     5101 ggcaaactgg tctccacggc tggtgcgggt ggcggttttt attcttttgt tctgtgcact
     5161 gatatactgc tgacgcgcct ttttcagctg acgggccagc acttcccagg tgtatgcagc
     5221 cagtgccgca cgttcccggt tgccgtagaa ccccacagac ggatgcgtgc cggggtgaat
     5281 gatggagtta acaccaaata cctcgcggat gatgttcatc aggcccagca tgtagcgcgg
     5341 tggacggaga ctgcctgtcg gccagtagtg actgatggtt tcatcaatat cactcatagc
     5401 aatgtcggaa tgtgtgatgc cgtgaacatc catcagttta cgggctcggc gcagtgccag
     5461 agcggcctcg tgcgggttgc cggatgcggc cagcgccagc aactttttca gtttctcaat
     5521 gtgtttattc tggtctgtca ttgttctgta tctccggtat ttttctgccg tttccatgcc
     5581 cggacagcgt cggacagctc tttcaggctg tatgctgctt taagcttttc ccataaccac
     5641 tgtgttgtta tgtgcatcag cagggcagcc agggctgccc ccgcactgag gcatgtcgcc
     5701 aggccagtaa gaatcagtgt ccatgcggtg atttccctga gtatgtcagc cattgaatgc
     5761 ctcccggtta ctcgttcgtg taaatcacgc ccagtcgtgc agccagacgt tccagttttt
     5821 tctgtttgtg gaagtcaatc agccggtcca tcccctgaag gcgcagttgc tctgtcatga
     5881 tttccacgtc tgccagctct gccgcgaggt cactttcgct gccctgtccg ttcaggttgc
     5941 gggcggcact ggccgccagt tcagcggcct cttctgccag tttcagggcc tgtgcgtccg
     6001 gcccgaaacg ctgcagggcc agacggtaga gggcggtgcg ggtgagttcg gtgtttcgtg
     6061 tcatgccgcg ccctcagtgc ttccggctga cggtgatgtg caggccgcct tctgcagtgg
     6121 tttccatccg gtacggcacc tcatgctctg ccgtgtgggt gagtgtgttc atcagtacct
     6181 gcagggcagc ctcctttccg ttggtcgcca caatggcctg tgtggccatg ctgattagcg
     6241 ccgtcaggac gtgcttcaca tcggtgaggt cgcggcattc acactcgttg acatagtgtt
     6301 caacaagggt tcgggtgcgc tgtcgtgctt cctgtggggt aatcattgcg agtcctccct
     6361 gtcagggcgg gagaattcca tgacgggcac gtctgccgaa aaatgctggc tgcagtacgg
     6421 gcagaccagg gtgacgcgta ccgcagggat gtggtattta ccggacatca cggcgatggc
     6481 gctgtgaaaa cgcagggctg ttatatccct ctcgcactga atacatttaa atatcatgat
     6541 ttaattctcc tctgtttccg gcgtgcagaa gcccgcggcg ctgacgtcgg aataaaaaag
     6601 aaaatgtttt tattaaataa ttaacgtggt gtgtttactg cacatcctgc tcaaaaggaa
     6661 ttattgaaaa atcctcaatg tcgcttttaa tggaaatacc gggaatattt ttcacggcct
     6721 ctttttcatt caggatagcg tctttattta tttcctcttt tacacgaata aagcgctcaa
     6781 gccccagacg tctcagtaat tcaataacat tatccgcccc acggatactg actgatggcg
     6841 gacggtttcg ccactgcacc tcgccggtgg tgaggttagc gaacttcacc ttcccgttgc
     6901 cggtcagttc atcacggtgt gcctcacacc atgtctgaat accggactgc agttcggcca
     6961 tgcgtttttt caggctctcg gtgagcgggg cataacgtgc ggtgatatcg ccaatggcgt
     7021 cattcatttc tgtttcagcc ctgaccagtt cacgttgtgc gtcaccgagt agtctgattc
     7081 cctcaatgac ctcttcacgt gtccccggca cccagagtgc tgctgcggcc ttgatacgtt
     7141 ttgcaccttt tgtacttttt gccatttttt atgatttctc cagttgtgct gattaccaca
     7201 aagattccgg ccacacgacg cgacagccgt gcagttcgaa aacgccctga cggaaatatc
     7261 ccctgtggtc atgtccggta tacagataac aggcctttcc ctgctcaagc atgcgcatgc
     7321 aatgcgcact ccgggaaacg cggatgacag gtttgttacc cctgatggtg atgctttgta
     7381 catccgtgtt cgtcgcctta agcgccataa tggctgactg cactttgctg atttgctggt
     7441 tgatacctgt ggtggatttc attattaaac ccctttgaca acgtcagcgt tgacctgtgg
     7501 aaccccgatt tcagcggcca gattcatggc ggctattacc aggttactga cggccagcgg
     7561 atacagcagg ctgaccatat ttttacggtg acttcccgga ttgctcaggc gggcacgtat
     7621 ggcatccact gcaccggcgt ccataatgtc cgtcagctgt ttaccggccc gttgcagttt
     7681 gaacgtcaga aattcttcaa ggttattgtc cagaggcaga agttcgacca cctcacagcg
     7741 ctgaacgact tcacggactt ccatattgcg ttcagacagt tttgtcgcca gttccggctg
     7801 gccaatcagc acgatggaca gcagtttttt gaaaccggac tccagctcaa aaaagcgttt
     7861 gaggtgtttc agtgtcggaa tgggcagact gtgggcctcc tcaatcacca gaacgtggct
     7921 gaaccccgcc tggctgctgt ctttcaggac gcgatgcaac tggcgaaaac gggcgtcctg
     7981 actgcgtctg atgctttcca gtggtgcgat ggtactgata atggcttcgg caatagctgc
     8041 tgccttcagg gttttccctt tcacatcgtt gtcttccata gcgatgatgt atggctcgat
     8101 aacaattacc ggcgcattct cgcggttgat acgttcagtc aggtcgcggc gcagcgtgga
     8161 tttacccgca ccggactcac cgatgacggc cataaaccca ccatgacggg ctgtctggta
     8221 cagcgcctca cgcacgtagc gaatgtccgg ggtggtgaac acatcatcag aaccctgcat
     8281 ggcttcgtcg gcgaacgggt cacggaaaag accaaacgct tttttggttg ctggaaataa
     8341 cacctgtttt ttgagtaaca tattctcttc ctcactgagg ctcgttttat ctgtggtacc
     8401 cgctgtacgg ggcgtggccg cgccctgtac agcatcaaaa ctcttcgttg tatcaatccc
     8461 ctgactttcc agccaggacg caagacgccg gcgcacttct ccggggctgg tgcggggcca
     8521 cgcgttatga ttcacaatct gggccagcgt ggcctcagaa acatcgacag ctctcgccac
     8581 caccgcctgt ggaatacggg cctctttcag ttgctgcttc agtaccagca tgtttccctc
     8641 ctcagttgcc gttaacaatg ctgataacac tgctgcgggc cggtgtggtc agcgtggcca
     8701 tgacttcatc cagtgcggct tccggtacgc cgtccgggta ctgtgccgtt aactggcggt
     8761 aatgttccgg cgtccaggta aggccgtcgg cgctgaactt atcgcgcagg gctttcgcgg
     8821 cctccacatg agtcatggga cgttgttcag tgcgcggccc gcgtacgtca gaggcctgac
     8881 cacgcttcgg catataggcc ggaagtgtgg tgtcgtcgat atgtttatac gggtcaagcc
     8941 gcccgccgaa cggcagcgcc ttcgccttgc gtgcggcggc tgcatctgcg gcgttgtctg
     9001 taccggtaac cagcgcttcg gtttcttttg ccgccatctg tgccggggtt tccggcaggg
     9061 ctttgtaact ttcgccaaat accgccgcgc cttcagcaaa gccaaactcg ttctttctga
     9121 cctcttcgac caggaagaac gtctcgtggc cgtcctcacc ggtcagaacc acctgtgcca
     9181 catcgctgcg ccatgggtta cgggtaatca tcagtttttc accaaccagt acgcccggta
     9241 ccgttgatac gtcaaattca gtgccccgga acgagacacg aagttttggc gtgactttgc
     9301 ggagttctgg tgccgccaca gccagttcac gacatacctc aacggaaggc gcttttttca
     9361 gctgctcagc agtaatcttc agccagatat ccgtgcgggt tttaccgtgg cggctgtgaa
     9421 cagccgtggc gttaaagtgg ctgcgccatt tcgcggccag cgcgttgagt tcttccagac
     9481 tgtgaaccgg ccggaacttc agacccggct ccagcttgcg ttcgataatg tcacgcgcct
     9541 tttccacctg tccggtggcg cgggcgttat gcggcttgtg cgctatcagg tcgatgccca
     9601 gtgagcggca catgtttttc gtcatacccg cggtgtttgc cgagccgggg tcgagataga
     9661 gtattttcgg cacgccgtgc agcacgtctg cgccgccacg ctcctgcatg gcgttgataa
     9721 gcacagaaca caggttctca cctgattccg cgcccgtcac atactcaacg taaatccagc
     9781 cgctggcatg gtcggtaatc tcgtaactcc acacgcggtc actggcgata cgggcaaggt
     9841 tagcgggctt gttcttgtag aacttcgcgc tgtccatcac ctgcagccct ttatgtccat
     9901 tgctcaggta gtaaagcgtg cagagtgagg cgtcaatctg ccagacatga ttgggatgca
     9961 gactggccac ttcggtatgt ggtgcagggg catccagttg ttccgggtgc aggccatagt
    10021 tacgcagagc acggctgatg gcatcttcag acaacgggaa aaactcactg gtggtttcat
    10081 ctgttctgcc tgcggtgata aagccgttag cccgcagggt ttccactgca tctgcgatgg
    10141 aatagagacg cttaccgttc ttgcgggtgg cctcacgcag tgtggcagat atcagcgcgg
    10201 cttcgtcgcg ggtcagggcg ctgcgcccgg catcggcgcg ttttttgcgt ttatcagtca
    10261 cagatacctc cttcagcctg cgcagcagag tggcgcggga catgccaagt tcagcacagg
    10321 cagcgtcgta tattgcaccg cgtttaccat gccccgcgtc acgtgccgcg cgggctgttt
    10381 caaccagttt ttcagtcagt gccggggtca tagcttgtct tccctgttat ccgattctgg
    10441 cggcaatttt tctggcgagg tcgcacagtg tttcgccatc gcaggttttc accacggtat
    10501 ttaccggttg cggtgcaact accgggcgta atgctgactg attctgcaga cgtcttatct
    10561 gctgcatcag catcacctcc atcgtcagta caaacgggtg cgtctcctgt gcgggtacgc
    10621 agatttcacc ggaaggggtc accacgcgga aaccctgcac catctccggc agcagattac
    10681 acagtgcgtg ccccagtgcc tccgggctgt ccatcagggt attttgtgtg ttattcgtat
    10741 tcattactgc atatcctccg gtcggttgat ttcaggttcc ggttcggtca gccaggcggg
    10801 ggcatcgtta cccgatggag cggcctcaag gtcaaacgtc tcacgtaatg catgaacact
    10861 gcgttcaatc tggcagacca gaccggccat gaaatctctg ggggtatcga tcatgttttt
    10921 ggtgcagtat tcacacaggg tctcaaaaac actggacagc cggacggcga tagccgattc
    10981 ggcctcaacc gctaatgccg tcacttccgc ccgcaatttt ttcagttctt cgtctggcgg
    11041 cggaggttgc aggcgcgatt tcttctccag ttttgtggcc agttcgttga ttttttcgtc
    11101 ctttccggca agcacctgtt gctgagctgc gttggtttcg cgcgcttcac gcagggcggc
    11161 cttcagttcg cggctggtca tgcgatcaat atcatcaagc gttgcaccgg cgacggttcc
    11221 accatcagct aatgcgtcga gttcttcgtc atccagaacc atcagttcat ataacttggt
    11281 ttttcccaaa acgctcagcg ctgagcgttt tggctgttca tctccattgc cgagaaattt
    11341 cacgctggcc tgcatcatgc gacgagctac ctgtggtgct aatccgaggt cgttttccag
    11401 aatatttgtg aaatcaccat gcggttcatt ttcttttaag atgatcagcc gcttacccgc
    11461 ttccaacata gattcagcac tctgcgccat ataaaaacgt gcttcgtgaa cgatgcgttc
    11521 acgttcataa ggcagtccat caccaaactg ctgcatgatc tccatgcgat gctgtgtcat
    11581 ggcattcagg ctgacgttga gatcatccga cagcggtaca tcggcgttca gttcagtgtt
    11641 aatcggtgat ttggtacgtc ccattgatta ctccttacaa acgactacct gaaatgacac
    11701 gttggttaat ttcgttaata cgatcctgtg cacgtgccat ctcgttactg tgggccatgg
    11761 cgatctgcag taactggatg cccggtgcaa aacgtccgtt ctccagtttc agagccagcc
    11821 cttcttcaat gagggtgttg agtgcccggt tgatgtttgc cggtgactcg tgcagtgctg
    11881 tcgccagttc accgttagaa ataccgttca gggcatgacc gcgcagcgct ttgagtaccc
    11941 ggaggatgcg actgccggag cttgaaacat tcgtcttact catctgcata cctcttttgc
    12001 gatatgtgat aaccttttac aaagactcaa aatatttagg ccgcctgagc tgttgatttc
    12061 agtcccaact tcacggcgat ttcatgggat ttaccgtaac gggctttggt ctgcccgttg
    12121 agaacgcggt atacctcatt gcggttgtag ccgttttctt ctgcccagcg ggtgaaagtg
    12181 atcccgcgct ggcggaagag agctttgact tgttctgcag tcatcgttgt ctcctttgtt
    12241 gatgcaatga tgtttgcctt atatgtgtta gattattgct cataattatg agcatatcaa
    12301 gatagagatt cacttttatg agcaaagata taggagagcg cgttatttct gtgaggaaga
    12361 aagcagggtt gaaccagaga gaatttgcag ctcgtttagg tatttctaat ggtggtatca
    12421 gtcagattga aaatggaaag gcgatgcctg gaggggagtt tctgttaaag atgcatcaag
    12481 agtttaatgt tgatgtaaat tggttgctca ctggaatgag cacagatggt atgactagaa
    12541 accagaatca aatttcccca gaaaaacaaa aactaataga tacttttgac gacatgacac
    12601 cagaccaaca acgctcattt cttgagattg ggcgattcta tactcagcca aaaccaggaa
    12661 aaatcgcagg ctgatactga cccggacaca aaggacaggt ggttgatctg gatgctttca
    12721 gaaaacatcg ccagtgacat agtggaagtt gtttggttta ccggaaagca ttgtgctttc
    12781 cggttttgtt tcagaagttg cagattagca gttcacggcg cggcgtgggt ttacccgcca
    12841 gactgtagtt gatattcact gtctgtatgt tcagtccagt gaacacctcg cgcatctgcg
    12901 ggatgtcgtt caccgaaatt atcatttttc ctctgatgca ccgggccagc tctgccagcc
    12961 gcgagtagtt cccttcagga aaatccacac catagccttc cgtacccagg tacggtgggt
    13021 cgcagtagaa cagcgtgtgc ggacgatcat aacgctcaat gcactgttgc cagtccatgt
    13081 gttctatgac tgttctggaa agccgcaggt gtgccgctga cagttcttct tcaatacgta
    13141 gcaggttgaa gcgtggcggg gatgtggtgg aggtaccgaa gctgtgctct gccaccttgc
    13201 cgccaaacgc ctgcttctga aggtagtaga accgtgccgc acgctggata tccgtcagtg
    13261 tttcttccgg ggtgatctgc agccatttgt agatctgacg gctgaccagc gcccatttga
    13321 actggcggac aaattcttcc aggtgatgct ttaccacccg gtacagattc accagttcac
    13381 cgttgatatc attgatgact tcgatcttgc cgggtgtctt aagaaaatag agcgctgctg
    13441 ccccacaaaa cggctccaca taacattcat gcgccggaaa cagcggcaga atgtgttttg
    13501 caaggcggcg tttgccacca atccacggca cgatgggtaa tgtctgcatt ttcataatct
    13561 gcaagccttt tacaattatt aaaaaatatg gcagtctggt caggtctcgc gagactgact
    13621 gaaccttggt cggctcacag cgcattcctg tggggcgatg accagcccgg ctttcgggtg
    13681 ccgggctggt cgttctttca tgatgcggca gggatggcgg gatttctgtt aatgtggttt
    13741 agggattatc cggggcgatt ttctgacctt atccggtcca gggcataagt aataccttct
    13801 gcaaaaacag aagtatcaaa ctgcttttgt attccctgaa aatgaaaagc ggcacgacag
    13861 acactcagtt ctccgcggta taaccggata atatcttcca gaacattgtt cactgcttca
    13921 gcacggcgct gatgttctgt taacgttaat cggggattca tgatgcactc cttaaatgat
    13981 gatttttatg tactcaccgg caaaatattc tgccgattac tgaacacgca tccggcacct
    14041 caggttttga tttattctgg ttttttcgat atcccggacc aggttgatca cgaccagaaa
    14101 aagtgccggt gttttattga tacgctgata tggcttcggg ataacagcta tattcgttac
    14161 tctcagttgc tggctgtggg agcggctgat gtcgtcctca cggaaaaaac gctctgtctg
    14221 ctcaatgcca tgccgacctt tctgcagccc cggcccgtac catttaaaga gtcacttctg
    14281 gatgcagtaa aaaccggtga aaatgcagtc atcgctgaat gcgtatcccg gattatgcgt
    14341 tacgtgttta atgaacgtaa gtgaggttta tccgctgtct gcataatccc gatagccagt
    14401 gcgaggggaa gtaattccgg ggaaactctt tcaaacagcg atgcccattc tcctctcgcc
    14461 atcactctgt ccagcccgcc ctccagttgc atttcagcaa tcttcctgat ttcccggaac
    14521 aacgccgctt tgatctcttc atgctcagct ttattttccg gtgtcagaag gtattcgtcg
    14581 aagaaccggg gaggctggtt aatcgggatg tgttcctgtt tgccgtactt cggggacgaa
    14641 caacattctg ttgactgacc agtgagttta cgtaactcct tcatgagcag ctcctgatcg
    14701 taaactgtca tcagtgccgc aatggtggca tgcagtgcat cgggcagtat ccagggttgc
    14761 cggcccgtgg ggcatataac atccagcagc tcattcgtcc gacagatacg ctcctcgccg
    14821 tatgacaaca ggccctgtcc cgtgtattcc tccagcctga tcactgtgcc agcccgtggt
    14881 ttctgccatg tgctcagatc atccccactc atacggcgct caccgtccac gtctgcggcg
    14941 tcgacaatga cgctgtttcc gaattcctgt ttcaccactt cctctattcg cgccagtacg
    15001 taacttttac cgctgcccac aggaccggac acggtgacag tgatgaccgg attcagtgtc
    15061 tgcgtcatat cgtattctcc tctgtgaata attcatcgtt tatcctgcac atcccgtatc
    15121 cccggctctc ttaacgcgct ttaaaatcct tcgcgccctg tatttgtgat gctgtctcca
    15181 ccagataagg agacacacat gaaaaacctg aaaaaattca ttccccctgt taaaaaacct
    15241 cgcctcagcg gctggctgct gacctcagtg ctgttgctgg gcaccatcgc tctggtctca
    15301 ccacagcagc tgcctgttgt gatctacaag ctggcactca tcacgctggc agcagtgctg
    15361 ggttactggc ttgaccgttc gctcttcccc aaagcccgtc ccggtcagta cctgaaacat
    15421 gacgacaggc tgatggctga agggcgtttc ccggtacaga ccggccttca cctggtgttt
    15481 tctgctgcgt taatccgccg tgcactgatt gttgcagcgg tctgcctggc tgttgcgatg
    15541 gggctttaat catgaactgg ccgcagatca cctggattgt atgcatgtcc ctgaaactgg
    15601 ctttcgaagc ctttcgggtc ttcatcagaa ccgaacgcct gtcagtccgc gccgggacct
    15661 ttctggttca tatggcatgg gtattctttg tcgcaatgtt gctctggtgt ggcggctttt
    15721 tcagccaggc atgcgcagca caacctccgc aggctgcgct gcagtatcgc gatgatgtga
    15781 tccgtaatgc ccggcttgaa tggggactgt ctgcgccggt ggccgatttc gccgcgcaac
    15841 tgcatcagga aagcggctgg cgacctgatg cgatctcgcc ggctggcgct cagggactgg
    15901 cgcagttcat gcctgccact gccgactgga taagccagtt gataccgatg ctgagcagtc
    15961 gtgagccgtt taatccggca tgggctatcc gggcgctggt cagctatgac cgctggctgt
    16021 ggcagcgtgt cagcgccgcc agtgactgcg agcgtatggc catgacactg tcgggctata
    16081 acggtggtct gggctgggta caacgggaca ggcggcttgc atcacagaaa ggtctggaca
    16141 gcacccgctg gttcggacat gtcgccacgg tgaatgccgg acgcaatgcg gccagctggc
    16201 gggagaaccg ccattatccg cagcgcatcc tgcgcgaact ggcaccgcga tatctcacat
    16261 ggggaggcag cagttgtgtg gcatctggtt aaaaagctgc cgtggcgcgg cattctgctg
    16321 gccattctta tcaatgcctt tctggtcggc ctgtatgcca tgggatacag aagtggtcat
    16381 gactctgcaa agcgtgacgg tgataccgcg ctaagtcagt tgcagtcagc atttgacgcg
    16441 tacaaaacgg agcaggcaac gcttgagaat gctgcgctgc gggcctgggc cagacggtat
    16501 caggagcagg tggccgccgg gcagcgggct gaagccggtt atcttgagca gattgctcaa
    16561 ctggagagcc ggaacaaaca actacagggg caaattaacg atgtcacaca gcgctggatt
    16621 gatgaaaaag gtaagagtca tcccattgag tgcgtgttta ctcgcggttt cgtgcgccag
    16681 tacaacgccg cactcggata tgacaacgca tccgtcgaca ccggtcattc agactcagtt
    16741 gccgccactg gcacccgctc tggcacagcg accgggcaac ctgaaaccac tgacacccgg
    16801 ttacgcgatt cgggtgtctc ccagcgtgac gttctcgcca acatcatcga caacgcatgg
    16861 caatgtcgtc gctggcggaa ccagataaac gcgctgctgg atgaacggga aggattacag
    16921 aaatgacact gcaggttgaa ttctggacgg tggtgagttt tctgctcacc ttcatgaggt
    16981 ttgtgggagg gctcgccaaa tggttgttca gtaaaacaga agaacgccag gcggcacgat
    17041 tcgcctccct tgaacaggcc ctgcaacaat ccgcctccaa ctggggcgag ctggaaaaag
    17101 aatttatgcg atttaaagcg gatttaccgc tgaattatgt ccgtcgagag gattatatcc
    17161 gtggccagac agtcattgag gccaaactgg acgcgcttta taacaaactg gaagtggtac
    17221 agcagtaccg caataccgga ggtcaataat ggtcgatatt acccgggtac gccgcgaatc
    17281 cctgcgctgg agtctgctgg ttgccctgaa caagacccgc ccttacaccg ccagcgagac
    17341 gctgctgctg gatgtgtccc gtgccatcta cccggacacc acaccgctgg aactgcgccg
    17401 cgaactggat tatctggctg accgtaaaat ggttgagctg gagaaaaaac cttctggcga
    17461 ctggtttgct gacctgaccc gcctcggcgt agacctggtg gaatacaccg tggaatgtgg
    17521 tccgggtatt gcccgcccgg aaaagtactg gagtgaataa tggccagacg cagcacaata
    17581 gaaaagctgc cggaagacgt gcgccgctgg cttgaacggg cgctgactga atccggcttc
    17641 agcgggtata acgagctgga gtcactgttg cgtgagcggg ggtacgtcat cagcaaatcc
    17701 gctatccatc gctatggaca gaagattgag cgccgctatg gtgctatccg tgcggcaaca
    17761 gaagcggccc gcatgctgac cgaaggcgca gcagacgatc aggatgcgcg ttcggaggct
    17821 gtgatagccc ttattcagac cgagctgttc gagagtattg tccagttgca ggaggcggaa
    17881 gaaggcgaag tcgatcctaa agaacgcgtg gccctgctgt cgaaggtggc gaagaatgtg
    17941 gctacgctgt cccgcgcgtc cgtcaacctc aaaaagttcc agtctgaagt acgggccaga
    18001 gcgcagcagg cagccagcaa cgccgagaaa attgcccgta aggggggact gtcaagcgac
    18061 gcagtacagg cgcttcgtcg cgagattctg gggattgcca catgacaaaa tcatccggag
    18121 tgattttaaa cgccgctggc ggcggccccg aagggatgag tcccatggac gggaggaata
    18181 accttgctcc cgttttgcct gatacctcgg cgctggatgc ccctcccgtt ctgttgcctt
    18241 accagcagcg ctgggtggca gacacctctc cgcttaaggt gatagaaaag agccgtcgta
    18301 ccggtattac atgggctgag gcatccgata acgtactgac cgccgcctct tctgcgccag
    18361 caggcgggat gaatgtgtat tacatcgctt ataaccagga catgaccgtc gaatacattc
    18421 aggcgtgtgc gatgtgggca cgggcattca actatgcggc cagtgaaatt gaagaaggat
    18481 tctgggaaga ggacgacgac gacaaacaca tcaagactta caccatcaaa tttcctgact
    18541 ccggctttcg tattgttgcg ctctccagcc gcccgtctaa cctgcgtggc cgtcagggta
    18601 ttattgttat cgacgaagcg gcgttccatg agcaactgga cgaactgctg aaagcggcgc
    18661 tggcgatgct tatctggggg ggaaaggtac gcgttatctc cacccatgac ggtgacgaca
    18721 atccgttcaa tacgcttatc ggggatatcc gtgccggacg tcagggaggc agcatacatc
    18781 gcatcacttt ccgggaagcc gtatctgagg ggctgttccg gcgcgtctgt ctgcgcaccg
    18841 ggaaggaatg gtcggaggca tccgagcagg cctggatggc atcggtgtac aaattctacg
    18901 gtgccggcgc atccgaagag cttgactgta ttccggccaa cggtggcggt gcctggctgt
    18961 cccgtgccct gatagagtcc cgcatgtccg ctgatacgcc ggtattgcgt ctgacctgca
    19021 aggaaggtta tgaactgctg tctgatgagg ttcgcttccg cgagacgcag gactggcttg
    19081 atgagtatct gaaaccattg ctggaggcac tccccactga tgcccgctct ttcctggggc
    19141 gcgactttgg ccgtagcggt gatttgtcgg tggactatcc cctgctgcag gagaagaacc
    19201 tggtacgacg cgtgccattc gtactggagc tgcgtaacgt gccgttcaga cagcaggagc
    19261 aaatcacctg gtatctgatg gatggcctgc ccggtctgct gggtgcagcg tttgatgccc
    19321 gtggtaatgg tgcctatctg gctgaatacg ccatgcagcg ctacggctcc ggccgggttc
    19381 agcaggtgat gccaaccgaa ggctggtacc gggagcatat gcctccggtc aaagctgcac
    19441 tggaagacgg caacctggtg gacttaccaa aggatgaaga cacactggat gacctgcggg
    19501 ccgttcaggt ggtgaacggt gtcccccgcg taccggagca acgctcaaaa gcaaaggctg
    19561 acggtggtaa acgccacggg gattcagcca tcgcactggc gctggcgtat ttcgccagcc
    19621 gtgaaattaa caaagggccg gtgaaggcaa gctcacgccg tcgtcgtcag gcggcccgta
    19681 tgctggaggg attctgatgg cgaggggtat ctgggtttca cccgatgaat ttgttgcttt
    19741 ttctgagcct cagaaatcac tgaccgcgca gattgcctcc cgcagccgcg cgatcgactt
    19801 ttacggactg ggcatgtatc tgcccaatcc tgatcccatt ctcaaggctc agggacggga
    19861 tatccgtatc taccgcgaac tgcgcaccga cccgctggtc gggggctgta tccgcagacg
    19921 taaagcagcg ctcaaatcgc tggagcgtgg actggagcgt ggtcacgcct ctgcccgggt
    19981 cttccgtttc atccgcgaca tgctcgacga tctggatctg tcccgcatca tcggtgaaat
    20041 gagcgatgcc gtgctctacg ggtatcagcc ctgtgaaatc atgtggggcc gttcggtcag
    20101 gtcgtgggcg gtgacggata ttgtcggcaa accgcctgag tggtttcagt ttgatacgga
    20161 caactgcctg cgctttcggg cgcgtgatgc gggtgtggag ggtgagctgc tgtcaccgtc
    20221 aaaattcgtg gtgccggcac aggatgcctc gtatgacaat ccttacggtt tcccggacct
    20281 gtccatgtgc ttctggccgg tcgccttcaa gaaaggcggg atgaaattct ggctccgctt
    20341 tgccgaaaag tttggctccc cgtgggtgat cggtaagcac ccgaggggcg caaatgatgc
    20401 agagattgaa aaactgctgg actccatgga gcagatggtg gaggacgcgg tggccgccat
    20461 ccccgatgac agcagtattg agctcagggc agcggatggc aaggcagaca gcagcgaggt
    20521 atttcgcgag ctgatcacac tgtcacgcag tgagatctcc attgcattac tcggtcagaa
    20581 tcagaccacg gaagcgaaca gtaacaaggc ctctgcacag gccgggctgg aggtaacggc
    20641 tgatatccgc gatgcggatg cggacatcat tcaggcggca gtgaatcagg tcatcagaac
    20701 ggtggtcacc ctgaacttcg gcgatgtgcc gtgtccggtc tgggccatgt gggaacagga
    20761 ggccattgat gacacccgtg ccacccgcga cgaaaaactc acccgggcgg gtctgcgtct
    20821 gaccccgcag tacttcaagc gtgagtacca actgcaggac ggcgatattg acgagacacc
    20881 accgtcggaa cgccagaata acatgctgcc gctgtcattt gccgaggcga ttgatgctga
    20941 tattcaggct cagcagcagc ttgacgacgc gctggacatt ctgatgaacg gaggcgcgtt
    21001 aaatggcacg ctggaacccg tcctggcacc tctgttcagg cgggtcgaaa acggggttaa
    21061 cccgtctgag ctgctgggcg aactggcgga gctctaccct cagatgaaca cggacgatct
    21121 gcaggagcgg ctggcccgca ttctctttgt ggcaaatatc cggggacgtc tgcatgagcg
    21181 tgacaacggc tgaactggcg tactgcatga cgcttccccc gaagcgggca atcaactacc
    21241 tgaagtccaa aggatatcag attacctggg actgggaaga aatgtggcag gaggcccatg
    21301 cccgcgcctt taccgttgct aaagtgaccc gcctggatat cctggaagat attcgcgggg
    21361 cactgcagca ggctgtcgat gaaggaaaaa ccgatcgctg gttccggcag gagctggagc
    21421 cggtgctgaa gcgtaaggga tggtggggac cacgtgacac gactgacccg gtaacgggtg
    21481 agccggtcac cattcagcag ggcagtccgt ggcggctcga taccatcttt cgcaccaata
    21541 tgtccgtact ctacagcgcc ggtcgttggg cggaacagat ggaaaacgtc gacgacaggc
    21601 cgtactggat gtataccggc atcaacgaca gccatacccg caggagccat ctggcgctgc
    21661 atggtctggt gctgcgctgg gatgacccgt tctggcaggc attttacccg ccgaacggct
    21721 ggcgctgccg ctgtagtgtt attgccctga gtgcggcgga tgtacgtgcc cgtggcctga
    21781 aggttatcag ctccggctct gccatgggcc aggaactgaa actggtctca gagaaaaccg
    21841 gcgaaatgcg gaacgtggcc acctttaata ccggcaccac gaaggtgacc accgacgtcg
    21901 gctggtctta tgctccgggg gcagcatacc gtcccgacct ggcccgctat cagggtacgc
    21961 ttcagccact ggcacaacag gaactgagag gataacaatg gcttccgata acctggtcag
    22021 tatcaccatt aacgataagt ccctgcgccg gagcctccgt gcgctggatc ttgctgccac
    22081 agacctggag cccgcgatgc gcaaaatcgc cggaaccctg ctggcggaaa cacagtttaa
    22141 ctttcttgat gaggggcgtc cggggtggat gccctcgctg gcagcggaag aacgtgacgg
    22201 gcagacactg caggataccg ggcgtctgat ggggtcagta tcaaccgacc atgacgaccg
    22261 gcaggctgtt gtggggacca acgttgttta cggtgccatt caccagttcg ggggtaaaac
    22321 ggggcgtaat gagtctgttg aacttccggc ccgcccgttc ctgccggtga cgggggatgg
    22381 agaactacag cctgaagtgg taatccccat cctcgatacc attgtccgcc atcttgaatc
    22441 agcggcccgt cgctgagttt tctctcttca ggcgggtgat ttatcattgc cagcgaatga
    22501 ggggctgtat tacctttata aaggctttac agcctctgtt ttataaccgc ctccggttca
    22561 ccgcattgct ttccctgtcc ttctcccctg atgttttcta aagcagatta aaatcgccgg
    22621 gcctgcattt ctcacaaact gtctccgaca acataacgcg ggacagcaaa atgtcagcca
    22681 ttcacatttt taaagccggt actcataccg atatgcacgg caaaaaactg ccgttcacgc
    22741 cagacgatct tgccgcctgc gtgaaagcct atgacccgtc cgtccatgaa gcaccactcg
    22801 tgattggtca tcccagaacg gaagacccgg cgtggggctg ggtgaaagcc ctgtcgctca
    22861 gcggcgtcga tctgatggca gagcctgccc agctggaccc gcagtttgct gagatggtca
    22921 ccgacggacg attcaaaaaa gtgtccgcct ctttctacct cccggattca ccgtccaatc
    22981 cgaagcccgg cgtgctctac cttcgccatg tgggctttct cggggcacag ccaccttccg
    23041 tcaaggggct gaaacaggtg tccttcagtg agcaggaaga aggtgtggtg gagttcgccg
    23101 actggcaggc catcacgaat gcctccctgt ggggaaagct gcgcgatttt ctgatcgccc
    23161 gcttcagtct ggacgaggca gaaaaagtcc tgccggaatg gcagctcaac agtctgcgcg
    23221 aagaggcgta ccgcgacaca ctgtcgcagg atgcagcagg tgcacaattc agtgagacag
    23281 gcccggggcc gtcttccgca agtaacgagg aatcatcgat gacaaaagaa gagattgaag
    23341 cccttcagga ggagaaccgc cgcctgaagc agcaggctgc tgatcgcgat gcgcgtgatg
    23401 cacaggtcag acaggagcaa ctgcataagg acaatgtggc ctttgcagaa aaactggtcg
    23461 cagagggccg tctggctccc cgcgcctcct ccgtggtggt tgccctgctg gatgccgtcg
    23521 ccggtggcga caagccggtg gagtttgctg agggggaaag ccgcacaccg ctggccaccg
    23581 cctttcgttc attgctctcc gacggggagc cggtgatgaa tttcgccgaa caggccacaa
    23641 aagagcgtgt cggcgacacg gtgaaggtgg atgtggcaga gtttgcggaa gccgatcctg
    23701 agcgtctggc cctgcatcag aaagcagtgg ccctgtccaa aaaagaaggc atcagctatg
    23761 aggctgctgt cgcacgctgc ctgtaattta aggagagagc atgtctgatt acttaaaagg
    23821 taaacgtgtc gttgatccgg tactgaccag tatcgcccgt ggctataaaa atgccgcatt
    23881 catcggcgaa cgtattttcc ccgtcgtgct gacggacaag gaaggcgtgc gtgtaccgac
    23941 cttcgggaaa accgcctttg tggaatatga caccgagcgt gccgtcgggg cggacagcaa
    24001 tgttctggtc cgtgaaaaaa caggcacgct ggacctggtg ctgggtgaac acgatctggc
    24061 tgcgccggtg gactatcgcg agcaggcgga gtccatgttt aacgaagaga gcaaggccat
    24121 ccgtcgcgcc acgaatggcg tgaacctgcg ccgtgaactt atcgctgccc gtctggctca
    24181 ggatgaaaag gtctaccgta ccgggcacgt caaaaaactg acagccagtg atcgctgggc
    24241 cggtggtaag ggggacccca tcggggtgat tgaagccggt atggaagcgg tccgtacggc
    24301 cacggggctg cgtcctaacc tgatgaccat gggggccggc gtgatggcgc tgctgaagtt
    24361 ccacccggcg attcaggccg ccatcggagc caacgaacgc aagcgcatca ccacagaaat
    24421 cctgcaggac ctctttcaga tcgaagagat cgtcatcggt gccccagtct ccctgccgtc
    24481 catgaaagcg gcaatggata agaacagcgt gccggcggat atctggggag acaatctgat
    24541 gctgcactat gtcggcaaac cgcagccggg ggcggacagc gcggacgaga acgagccgtc
    24601 cttcggctac accctgcgtc gtaaggggat gcctgttgcc gacaaatacg acggagccgg
    24661 tggcaaggtg aagtactgcc gttataccga tatctacaaa gtcgccgtgg ttggtggcga
    24721 tgccgggtat ctcattaccg gtatcagtaa ataaggaggc gttatgggaa ccactcagca
    24781 ggtcattctg atcacaaccg taacggcagg ggcagcactg gcacagcagc gttttgtcgg
    24841 ggcagataat accccctgta aagccggtgc cgcagcgctc ggggttgccg aagtggatgc
    24901 tgttaccggc gacagcacgc cggtgagcgt tctgggcatt attgctgtcg aggccggggc
    24961 cgctgtcagc cgtggtgtgg ctgttcagtc agatgctcag gccagagccg tgccgcagtc
    25021 cggcgacggt aaatcctgtg gtattgcact tgatgaagcc gggggtgaag gcgacgtcat
    25081 tcgtatcctg cgcggggtgt gacatgtact gcaccctgga ggatttgctt gcgcaggtgc
    25141 cggagcggac gcttatcgag ctcaccagtg aagagatgga cttcgactca cctgcaacag
    25201 tgaatacccg tgtggtggac agctgtatcc gctatgccga cgagctgatt gatgcccatc
    25261 tgcgcggacg ctatatcctg ccactggcag agataccgac tgttctgcgg gacattgcca
    25321 tcacgctggt ccgttaccgg ctctacgccc gccgcccgga aggtgacctc ccggatacgg
    25381 tgaaggatga ccacaaagaa gcgctgcggc aactcaggga gttacgtgat aacaggctca
    25441 cgctggggct gccgtccact cagaaagatg tgcctgagcc tggcgagttt cgtgtacgca
    25501 gtcgcccggc cactttcggc ggtcgtgacg gtttactgga gaaatactga tgaacgttct
    25561 gcccgtcctt gatgcggtac tggcccggtt acgcgagaag ctgccgcaac tgcaggtgga
    25621 gtacttcccg gagaaaccgg ctgaatatcg cctgaaccat ccggttggcg cgttgctgtt
    25681 gagctatgcc ggttcgcgct ttgacaggcc ggatgatacc ggtgcggtga tccagtctca
    25741 gactatccag ctctgcgtca cggtggtctt ccgccagctc aacggtaaaa aaggggcgat
    25801 taatgtcctg gatgctgtcc gccgcattct cggtggccac accccgcccg gctgccgccg
    25861 ccgtatctgg ctgacccgcg aggtgtttat cggtgaagtc agggggctgt ggcagtacgc
    25921 cctcgacttc gcgactgaaa gcgtctttat cgaagacagc gatttaccgt ccggcccgct
    25981 gttaaccgaa gtgaactatg aggaaagcga gtgatgaaag aataccgcta ttccggcccg
    26041 gccagcggcg tcacgctgtc ggacggaacc gaaatcctgc tctggccggg gaagacggtt
    26101 tccctgccgg aggagcatga ctacgtgaag gtactggtgg cgctgaaaca tctgacgccg
    26161 gtacctgaag agactaaacc cgccggcaca ccggctgtgc agtcaccaaa gcgcagaagc
    26221 agcagtgaca gcgaagtgaa aacggaggac acccatggca gctaactatc tgcatggtcc
    26281 cgaaaccatt gaggtggaaa acggtgcccg cccggttaaa acggtgaaat ctgccgttat
    26341 tggcctgatt ggtaccgccc cgatggggga tgtcaatacg ctggtacagt gcctgtctga
    26401 gaaagacgca gcggcatttg gcagccagtt caccggcttt accattccgc aggcgctgga
    26461 tgcgatttat gaccatggtg caggcaccgt tctggtcatt aacgtcctcg acccggcgaa
    26521 acataaaacg gcgatcgagg atgaggtggt tacctttgac aaatcgacag ggcaggccag
    26581 actggcgcat ccggttgtcg ctaatgtggt ggtgaaaaac agtgaaggca gcaccaccca
    26641 cacggcgaac acggactacc gtgttgatgc gcaggcgggt gtgctcacga acctgggcaa
    26701 ggctattgag gccggtggca gcgtgaaggt gagctatgag tacgcggacc cgtcgaaggt
    26761 gactgcggcg gacatcatcg gcggggtgaa cagcgccgga aaccgaaccg gcatgaagct
    26821 gcttaacgac agcttcaacc tgtacggcta tttcgccaaa attctgattg cgccggtgtt
    26881 ctgcacccag aagagtgtcg cagttgagct tatcgccatg gcagagaagc tgggcgcggt
    26941 aacctacatt gatgcgcctg tcggtaccac ctttgcacag gctctggcag gtcgtggccc
    27001 ggaaggcacc atcaacttca ataccagctc cgaccgcgtc cgtctgtgct atccgcatgt
    27061 gaaggtatat gacccggtga caaacacaga gcgtctggag ccgctcagcc agcgtgcagc
    27121 aggtctgcgt gccagagtcg atctggacaa gggctactgg tggtcatcct ccaatcagga
    27181 gattctgggg atcaccggcg tggagcgcca gctgtccgcg atgattgatg acccgcagag
    27241 tgaggtgaac ctgcttaacg aacaggggat caccacggta ttcagcagtt acggcagcgg
    27301 ccttcgtctg tggggtaacc ggacggcagc atggccaacg gtcacccata tgcgtaactt
    27361 tgagaacgtt cgccgcaccg gtgatgtgat caacgagtcc attcgttatt tcagccagca
    27421 gtacatcgac atgccgatta ctcaggcgct gattgatgca ctgacggagt cggtcaacgc
    27481 ctacggtcgc aaaatgactg gtgatggtgc ggtactgggc ttccgttgct ggtttgatcc
    27541 ggcccgcaat ccggagacgg agctggccgc cgggcacctg ttgctgagct acaaatatac
    27601 gccaccaccg ccgctggagc gactgacgtt tgagactgag atcacctcgg aatacctgtt
    27661 aaccctgaaa gggggcaact gatgtcaaag attgagataa accgcatcac gaatgccaac
    27721 atctatctgg atggtactaa cctgctggga cgggctgagg aagttaaact ccccgatgtc
    27781 tccatgatta tgcaggaaca caaggcgctg gggatggtgg gtaaggtgga actcccggct
    27841 ggttttgaca aactggaagg cgaaatcaaa tggaacagct tttaccgcga tgcgatgctg
    27901 tctgccgcga acccgtacag gtcgctggca ctgcagtgtc gttccagcgt ccagcgctac
    27961 agctcgcagg ggctgattga cgaaatcccg ctggtcacct tcctgacgat tatgttcaag
    28021 aagaacccgc tggggacgtt caaacagcac gagaacgccg agttctccag tagcttcacc
    28081 tgcacgtata tcagacaggt actggatggt gaagagctgc tgcaactgga ctatctggcc
    28141 aacatcttcc gggtcggcgg tgttgatcaa ctgactgact accgtatcaa tatcgggggc
    28201 tgacggtgag tgttgaactg acggataaag gaggacgatg tgcggcactg ggcatgtcaa
    28261 atggtacgtg gtttaccctc cttgatattc cgggggtgga aacccttttt aatacccgta
    28321 aaaccaatga cccgattgac tgcacacgtt caaaggcccg caaactggcg gatttgattg
    28381 aagcatggga gcctcccgac cactggttct ccggcatcgg caaatctgag ggaaagacgc
    28441 ttctcatcgc tttcctgcgt aactgcaagg ggtttcgcac ttgctgacat cacaggggct
    28501 ccggcccctt cttcttaatc tcctttaata tccgtcacgc gcttctcccg acatactgcc
    28561 ctgaacttac acaggagcac aatcatgtca cagaccccat ccgatacttt taaattgtct
    28621 tatcccttca ccactgctgc aggcaccaga attgagctgg ttgaactgaa acgcctgacg
    28681 gtaaaagacc tgaagcaggt gcgcaaaatc agcaaaaacc cggcagactg ggacgaaccg
    28741 ctgattgccc gcagtactgg tcttctcccg gaggatctcg acaatatgga tctggctgat
    28801 tacctgcagt tacagaaacg atttcagctc atcacgggga tgggtgagag caaccagggc
    28861 gctgacgcag gcgcaggggc tgctggcgag atggttccgg tttcagccgg gggagattga
    28921 tgccctcgat actgacgatc tggagatgtg gctggagcag gctgaagagc aaatcaaaag
    28981 cgagtacggc gacaaatcat agtacagaca gccgccagta gcggctgttc tgcgttatcc
    29041 cctcacgtct tttcaccttc cccggaggtt aaccactatg tcgggacagt tttcagtcgg
    29101 cgttgttatc ggcgggatga ttggcagcac attccgttct gcaatgagcg gtacccgccg
    29161 tgcgcttgat tccctgagcg atacctcacg ccgcctgcag gaacgtcaga acgctttaac
    29221 ccgtgcaaca gaacgttatg gtcaactggg ttcttcccgg atgcagcatc tcaacagcga
    29281 gctgctgcgg gtaagccgca ccatggagca aattgagcgc cagcagcgcc gtctgtcagc
    29341 ggcatccgct accagtgatg cgctgaaagc taaccgcatg gcgctgtatg gtcaggggat
    29401 tgaagcgtat ggcatggcac agactgttta tcatacggtt tcccctgccg ttcagcagtc
    29461 catgtctttt caggacaaaa tgattgatat gtcgatcacc gcaaaatatg acaataaaac
    29521 gcgggatgca cttgccggac agataaaagg ctgggcgctt aaatacaatc agtatcagga
    29581 tgagctgcag gaggcgatgg gttcactcat cagcgacaat attgataatg tgtcagatat
    29641 cggttttctg atgccggata ttgcccgcgc ggcaacggca acacgcacgt ctgctcagga
    29701 ctgggcaaaa gtggccgcag tctggcaaaa ctccctgaaa ggcgcggcca gagattttgg
    29761 tgccgttcag aatattatgg cttatgccgg tgaccagggg tcatttgaaa tcccggatca
    29821 ggtcaagtgg atgcagtccc tggccccaat gatggcgggt attgccagtg gaaaagaggc
    29881 tgttgctgaa atcggggcca gtctccagat agcaaaaatc ggtgcaggtt ccaccgacga
    29941 agcagccaat aattttaaaa actttcttac caaaattttt gcccgcgata ctcagaaaca
    30001 gtttgctgat ctgggtattg atttgcaggg atctattgcg agttataaag ctgcggggat
    30061 ctctccgatt gaagggatgt tgagtgttat agaacgttac ctcaatgcca aaagccccga
    30121 agcgctggcc ggcttcaaat cagccatgaa aataaagaat gatacggcaa gagatgaggc
    30181 acttcaggct ctggcgaaaa actttggtct gggcgatatg ttcgcggata tgcaggtcat
    30241 ggcatttatc cgcccgatgc tggccaacat ggacagatat cgagagatcc gtgccggtgc
    30301 tctcagggct gcggataacg atttgcttgc cagtgcttat gatcagcggc tgaaatctcc
    30361 ccttgaagcc actaaaacac ttatggtcag cagtcgcgat ctggcaatta cgctgggcga
    30421 tcaattagct ccatctttta tttctctgac tcaggacttg ctcccactca ttcaggggac
    30481 aaaacactgg gtggcgactc acccgcaatt tgtcagtggg gcttttaagc tcatcagtgc
    30541 gctccttgcg attaagatag cgactgttgg tctcaaactg gggctgaatc tccttatttc
    30601 cccctttgtg aacgtctgga aaactgctgt tttactccgg accaactggc atcgactgac
    30661 taccgcactc ggagaaggtg gcaaattacg ctggcttgtc actggattca gccgactgac
    30721 cagcggagga ctgaaactca gcaaagttct ggcgggcagc ctcgttcgcg gttttatgag
    30781 cgctgcacgt gccgttctct ggattgggcg agcgctgatg atgaatccca tcggtctcgt
    30841 tatcaccgct gtcgcggcag cagcttacct tatctaccgc aactgggggg cagtcagtgg
    30901 ctggtttaaa cagcgctggg ctgacattca ggaagccttt aacggcggta tcgcgggaac
    30961 tggtaagctg ctgattaact ggtcgccggc aggcctgctc tataaagcct ttgcggctgc
    31021 gctgaaatat ttcggcgtta ctctgccggc aaagttcacc gacttcggtg gccatcttat
    31081 cgacggtttg attaacggta tcaaaaacaa atgggggtcg ctcaaatcca gtgtaaccgg
    31141 aatgggtgac agcatcagta gctggtttaa acaatgctgg gctgacattc aggaagcctt
    31201 taacggcggt atcgcgggaa ctggtaagct gctgattaac tggtcgccgg caggtctgct
    31261 ctataaagcc tttgcagctg cgctgaaata tcttggtgtt gatctgccgg caaagtttac
    31321 cgacttcggt ggccatcttg tcgatggtct gattaacggt atcaaaaaca aatgggagtc
    31381 gctcaaatcc agtgtaaccg gaatgggcga cagcatcagt ggctggttca gcgaaaagct
    31441 gggcattcat tcgccgagcc gcgtgtttat gggctttggt gacaatatcg cgcagggggc
    31501 cgccattggc ctgcagcgga ccactccgct tgcagctctg gccgggcagc gaatggccag
    31561 tgaactgctc cccagaatgc ccgtgagcat tcaggggcca gaaatacggg ataacacttc
    31621 aggtgttcgc ttcagtatgc cgttgcccga tatcaatggg tttatgtcgt ctgctaaaaa
    31681 tgcgatcgga gccgtaatca atagtttgtc ttccatgccc gctattccgc tctccacccg
    31741 ggcatcgatt ttaccagggc aacgtctggc aaatgagatg acaccggatg ttccccgtat
    31801 cccctcgcct gaaatcctgg ctgccggata ttcaggccgt ggtgcagctg caaccggcgg
    31861 tggaacgtct ggtggtatcc aggtcagctt taatcctcag tttttcctca atggcaggga
    31921 aaccacagcg cctgcaggac tgactggtgc cctgaatatg agtctgcatg agctggaaaa
    31981 aatgctggag cgtctgctgg ctcagaaaca acgtaaggag taccgctgat gtttgccgta
    32041 ctgggtgata ttgagtttga gctgattacc tactgggacg gcttcgaggc cacgttcggc
    32101 gtcgattatg cggagcatgc ccgcatcggg ggtaagcctg gtctgcagtt cgtcggcgac
    32161 aggctggacg aaatccagat aactctggtt ttccatcagc attattgtgt acccgatgtg
    32221 gagctggcga gactgcgaac agccatgaaa gcccatcagg cactggcgct ggtcttcggc
    32281 aacggtgact atcgcggctg gttcgtgatc accgatgtga ctgcaaccag cgagcagact
    32341 gacagtaccg gcaacgtgct ggctgtcagt gccaccgtgt ctctccggga atacaccggt
    32401 gacccgaaaa atcctctgca accaccggca atacgcacga agctcccggg tgtcggggcg
    32461 gtctccggtg ccataccttc accttcaggg gtggcgcagt tcatccgcaa cggcgtcaac
    32521 tatgcgaaac aggcgcagtc tgtactccag accactatca gcgccgttcg ggtgacacag
    32581 aaaatgaagg ataaccccgt tgtcgcactg acccgtgtgc cggggctgat gagcggactg
    32641 ggtaatatct ccggggctct ggggaaaagt gttccggcgt ttaacgcact ctctgaatcc
    32701 atgcccgatg ccatcagtct ggccagaaca gccagcgaag cagccacgta tgtacagcag
    32761 gcacagtctg cgctgagtgg tgtggacaaa agaaatattg caggtgctct ggataccgtt
    32821 tccgggcagc ttaacgccgt cggcacagca ttcaaccgca tgtctccggg attaagtgca
    32881 atggccgcca gaatactgac gaggagtgtg tgatgtttct tgaacatgtt acccgtgacg
    32941 gagagcgctg ggattcgctg gcatggcagt actacggtga cccgctgggc tatccccgga
    33001 ttattgccgc caatccgcac gtggccatca cgccggtgct gccctccggg ctgttgttac
    33061 tgatcccggt gattgaggct gaagaagccc gtacagaaga ggatattgcc ccatggctga
    33121 gataaacagc actgcgcaag tcacatcagc gttaaccggc gtcagcgatg tgctgacacc
    33181 ggtgttcact ctgtggtatc tgcagaaaaa catcacctct gatatcgcgc cttatgtcac
    33241 ccgtgtgacc tggagcgata acatcaaaaa tgagtccgat accattgagg tggagctgga
    33301 cgacaccgat ggccgctggc tggataaatg gtatccgggc aagggtgaca cgctgacgct
    33361 gaaaatgggc tatcagggcg agaagctgct gtcctgcggt acgttctcta tagacgagat
    33421 cgaagtgagt tcgcccgctt ccgttgtttc tatccgtggg gtggccacct cggttaacag
    33481 tgccctgcgg actaaaacca gtcgtggttt tgagaacacc acgctggcag ctgttgcggg
    33541 gcggattgcc agaaagcacc ggctgaaact ggtgggcagc attgagtcca tcagaatcga
    33601 ccgggtgacc cagtatgctg aaaccgacgt gggttttctg cgccggctgg ccagcgagta
    33661 tggttatgca gtgaaagtgg tcagtgacca gctgattttt tctcatctgg ccacactgcg
    33721 cagtcaggag ccggtcaggc agttaaaacc gcaggatgtg gcccgctttt ccctgcgtga
    33781 caccatcaac cgggtctata aatctgcaaa ggtaaaacac cagaaaagca gcagtaaaaa
    33841 actgatcgtc tacgaagctg atggtggtac ccgtgaaagc gacaaaaagc tcaaaggtgg
    33901 taaggttacc agcgctgact cacttaaagt taacagccgc gtcagcgacc cggacagtgc
    33961 ccggattaaa gcggattcag cactggccag acataacgaa taccagcaga acggctccct
    34021 gacgctgacg ggaacacctc aactgacagc aggcaacaaa attgaactgg tgagttttgg
    34081 gcagttatcc gggccatggc tcataaccac tgcccgccat gcgtttgacc gtaacagcgg
    34141 ctacaccaca gagctggaag tggcacgggg gccagtcaca agagggaaaa aacaaaaaac
    34201 tcagaaactc acggtttatc acccggatgg cagtacatcg acggtgatta aggagaagaa
    34261 aaaatgactg gtgtcactcg tcaggtcggt acggtcagtg ccgttgatgc cgacagggtt
    34321 caggcccgcg ttcgtctgcc tgaatgcgat aacctgcgca caaactggct taacgtgctg
    34381 cagcgcaata cccaggataa caaagattac tggctccctg acgtggggga gcaggttgag
    34441 gtgctgctcg atgccaacgg cgaggatggt gttattctgg gcgcggtgta ttcagacgtc
    34501 gataaaccac cgttcagtga caaaaatatc cggggaaccc ggtttgctga tggtgcagag
    34561 tacagctaca accggaagac gcacactctg atcatccggg gcggcattga gcatattgtc
    34621 attgagtgtg gtgctgatgt ggtattgaaa acacagaaag ccacgattga cgcaccggaa
    34681 accgaactta ccggagatct gcgtgtcagg ggtaagctga tttacgaagg aggcatggcg
    34741 ggttctggtg gtgaaggtgt taccgcgacc atccatggca atatcgagat taaagggaat
    34801 gtccatgcca cgggcagtat gttgtctgat ggcgaaaact ccagccacca ctcccactga
    34861 gctttttaaa cgcctttaat atcagcgttc ccgcacgggg gcaatactgc ccccatgaaa
    34921 acaacctcag tattctggca accagccctg caggctcccg gcgaaattgt ccgggggctg
    34981 gatgatatcc ggcaggcgat tcaaattatc ctgcggactc cccgcggcag cgacccgcat
    35041 cgcccggagt tcggcagcaa tctgcatctt tatatcgact ggcctgtaga ccgggccatt
    35101 ccgcatgtgg tgcgcgaatc cgtcgatgcc atccggcgct gggagccccg ctgccagctt
    35161 atgtcagtta aacccgccgt cgacggcgaa catcttacgc tccgggtgag ctggaaaggc
    35221 tcagacggac agacccggac tcaggagctg ctatggcgct gacagaaccc gattttattg
    35281 aacgcgatgc cgacaaaatc acggcagaaa tgattgcgaa gtatgaagcg gataccggca
    35341 aaacgctgta cccggcacag gcagaacgtc tgctgattga tctgtgggcc tatcgcgaaa
    35401 tgctggtcag ggttgcggta caggaggcag caaagcagaa tctggtcgcc tttgcccgtg
    35461 agccgatgat tgattacctc ggtgaactgg tcggtgtata ccgtcttgcc gcgcagcctg
    35521 ccaccaccac gctccagttc tccgtagatg aggcactggc cattgatgtg ctgatcccgg
    35581 caggcacccg cgtcagcgct tccgacagcg ttatttttgc caccgataca gatgtggtac
    35641 tgaaggccgg attgctgctg gtcaatgtca cggccacctg taccgaaccc ggtaccgctg
    35701 gtaacggctg gcaacctgcg caggtcagtc agttactcga tgagattgat aacgttaacc
    35761 tgctggtgag caatctgatg gccagttccg gcggttcaga acaggaagac gatgacaggc
    35821 tccgggagcg tatcaggctg gccccggagt cattcaccaa tgccggaagc cgtggcgcat
    35881 accgctttca tgccatgcag gcccatccca acattgtcga tgttgctgtg ctttccccgg
    35941 ttcccgggac cgtagatctg tatccgctgc tcagtaccgg tctgccggac ggcggtgttc
    36001 tcacgctggt agagagtttc tgctctgatg agaaagtcag gccactcact gatacagtgc
    36061 gggctaaaac accagtgaag gtggattaca ccattgaagc caggattacg atctatcgtg
    36121 atcaggatgc caggtctgta aaagatgccg ctaacagcgc catacagaac tgggtggcat
    36181 cacgtgccgc cacgctgggg cgcgatattg tccccagcca gattatcagt gcattgtccg
    36241 tttccggggt gtaccaggtt gaactggtga caccggcact gcgggtggtg gcagaaaacg
    36301 aatgggcaaa ctgtacggca atcactctta acatgactgg agtgtctgat gactgagcca
    36361 ttacaactcc cgccaccgct tgagggtgat atcagtctca ggacgctggg aagactggca
    36421 ggacggctgg ataacatcga cctgagcgta ctgatggtct ttctcgtcga tatcgtcgac
    36481 agttccgcgc tgccatggct gggcgagcag ttctcactgt ctggcgatgg ctgggagctg
    36541 gcggaatcgg acgatgttcg ccgcatgctt ataaaagcag ccatcgaact gcaccggtat
    36601 aaagggacgc cgtggtcaat ccgggaagtt atccgccgtt ttggctttgg tgaagtggat
    36661 ctgattgaag gcacaggtcg tctcagttac gacggcaatc gcagctataa cggactgttt
    36721 gttcatggag atgcagccgc ctgggcagtt tatcgcgtta ttctgaaaca gcccattact
    36781 aacgatcagg ccgcgatgct gcgtcagacg ctggctgcat ttgcaccggc ccgctgccat
    36841 ctggccagcc tggagtatca gtctgtggcc attcgctata acaataccgc catccatgat
    36901 ggcagttata accacgggag cagttgatta tgggaaacct gaatgaaaca gaaaagtggg
    36961 aagaaaatat ctatcaactg gagacatcag atccggttct gggtggtgca gacgggatat
    37021 caaatcgagc cccccggcaa ctggcaaaca ggacgaaatg gctgaagaag aaaacggagg
    37081 aagccgcaca gtcactggct gaacacgtac gttcccgtaa ccacccggac gcgacactga
    37141 cagctaaggg gttcacccaa ctgagcagtg ccaccaacag cacctctgaa acgctggccg
    37201 ccacaccaaa agcggtcaag gctgcatacg acctggcagc cggcaaggcg cctgccagcc
    37261 acactcaccc gtggagtcag ataacgggag tgcctgcggc ttcactgacg gcaaaaggca
    37321 ccgtacaact gagcagcgcc acggacagtc aatcagaaac tgaggctgcc acgccgaagg
    37381 cggtcaaggc tgcatacgac cttgcagccg gcaaggcgcc cgtcagccac actcacccgt
    37441 ggagtcagat aacgggagtg cctgctgcct cgctgacggc aaaaggcacc gtacaattga
    37501 gcagtgccat caacagcacg tctgaaatac tggccgccac accgaaagcg gtcaaggctg
    37561 catacgatct ggcaaacgga aaacaaccgg cagatgccac gctcactgct ctggcaggac
    37621 ttgccactgc agcagatcgg ttgccttatt ttaccggagc agaccgcgca gagctggcaa
    37681 cccttacagc tattggtcgc gctattatcg ctaagggcag tataaaagat gtcctcaatt
    37741 accttggttt gggggaaggc tctgcattgc ctgttggtgt accggttcca tggcccaccg
    37801 ccacaccgcc agcaggctgg ttacagtgca acggagctac gttcaccaaa gaacaatatc
    37861 ctgttctggt cagagtctac ccgaccctcc gtcttcccga tttgcgcggt gagtttatcc
    37921 gtggatggga cggtggtcgc aaggttgata ccgggcgtgc gctactttca tttcaggaag
    37981 ggactatcgt atccggtttt gatgataatg ataccggaga tatcagttcg ctcggctcta
    38041 cacaatacgg atttggtgac gctttaacct ctaatcaact gggggtctta aatggcaaaa
    38101 agtggatatt tgatgcatcc tctaaaggtg cacaaagata tgactggtgg gcttatgtat
    38161 cagctcgccc ccgcaatatc gcatttaact atatcgtgag ggcggcgtga taccgttcag
    38221 ccggcaagcg ccagtggcgc ttccggccag ctgatttcag gtgccttgct gatatccagt
    38281 gcgttcagct cgtcgatata atccagcacg gcgttcagtt ttgccgtttc ttctggtttc
    38341 agactgcgac cagcacgcag tttgaggttg ataagatcga cggacgccat cgcctgtttc
    38401 acccgttgct ggcgttgctg tctggcaacc tccagttctg cggctcgctg ccgttcagta
    38461 tcggttaccc acgcctcacc gttccaggta tcccatggcg tggctggctg ttttgtggtg
    38521 gtgtccgccg ggtaatcacc cggttgggta atcagaacgg ggttaccgtt ctccgtgctg
    38581 tataccgtct cgccgcggtg atcaggcaca tgctcccacc cgttcagcgt ggcagtccgg
    38641 caaaccacgt aacccgaaat tgcttccggc ggtgcatcgg cgcacgaatg tgccggagta
    38701 cccaccccaa gggcgagata ctccacagag gatgaggtgt attcccgggt ttcctcgtca
    38761 tagtgatata cagtgatatc gcccgctaca gtggcgatac cgttttcatc cagaactgca
    38821 gtgttactca ttaaactgcc ctcacaatgt aattaagtgc aatgctacgg ggtctgacag
    38881 tgataccctt cgcatacccg cttctgatag aggtcagaat agtagcctgc ataccattgt
    38941 ctgacattgc cacgacagga tcgctgaatg ttgtcccgtc cggcatttta aagctgccct
    39001 cacgaaggtc tgtaatggaa tcttcattgt tgaatcccat gccgactatc cccgaggttc
    39061 ccgtagtgtc ctggttataa taatccagca ttgctgttct taacagcgtt gccccttgtg
    39121 cggacagcaa cttacgtcct gtatcaactt tgcgcccgtc gtcccatcca cggataaact
    39181 caccgcgtaa atcgggaaga cggagggtcg ggtagactct ggccagaaca ggatattgtt
    39241 cttttgtaaa ggcgcgcccg tcgcacttta accagcctgc tggcggtgtg gcggtgggcc
    39301 acggaacagg cacaccaacg ggtagcgccg agccttctcc taaaccaacg tttctatgcc
    39361 gcccttacga tgtaattaaa cgcaatgtta cggggacgaa tggtcacata attaacccca
    39421 tcgcctcgcg aaggacctgc aaatgaaaaa cgggagaatc caggttggtt gtcaacgatt
    39481 tggtcatagt tattgattga atgaccagac ccaatgtcaa aattacctgc gtaatgagaa
    39541 aaacttgtac catcctgccg tgaaagcaga tcgcgccctg tatccacttt tcgcccgtcg
    39601 tcccatccac ggataaactc accgcgtaaa tcgggaagac ggagggtcgg gtagactctg
    39661 gccagaacag gatattgttc ttttgtaaag gcgcgcccgt cgcactttaa ccagcctgct
    39721 ggcggtgtgg cagtgggcca cggaacaggt acaccaacgg gcagcgccga gccttccccc
    39781 aaaccaacgt ttatctctca aatcctcccc acattatctc agtactttaa ccactcaaaa
    39841 gggagtattt ttaatgctga ttggctacat acgtgtgtca acaaatgacc aaaacacaga
    39901 tttgcaacgc aatgcattaa tgtgcgcagg atgtgaacag atttttgagg acaaaatgag
    39961 cggaaccaag tcggaacgac caggcctgaa acgcgcttta aagtgcctta aaagagggga
    40021 tacattggtg gtctggaagc tggatcggtt gggtagaaga atgaaacacc tcattgctct
    40081 cacagaagag ctacgcgcaa aaggtgtcaa ttttcgcagt ctgacggatt caatcgatac
    40141 cagcactccg atgggaaggt tcttttttca tatgatgggt gcactggcag aaatggagcg
    40201 tgaactgata gttgagcgca cgctggctgg actggctgct gcgcgagcac aaggaagagt
    40261 tggaggacgt cgcccgaaac tgacgaagga gcagcatgag cagatcgcaa gattgctcca
    40321 gaaagggtat gacagaaagc ggctggcaat tatctatgat attggactgt caacgatcta
    40381 ccgctatcac cctgttggga ctgtcataac gcaacctgaa atgtaattct ttttcaaata
    40441 atgaaacgcc gcgtggatgc catttatcgc acaagataga gtgcatttat cgcgcggcgc
    40501 atcattcttc atgtgaatca tccgttgatt aattatatat aaacattatg tatcaacaag
    40561 ttacattgat aattaacgct accattaata aaaatacccc acaaggtccg aaaaaaaacg
    40621 ccggcaatgt gccgacgtct ttgaaaagaa aggattgtct atgttatcgc ccgttattca
    40681 ttcagccggg ttgacttatc cgcgccaatt gctcgaaata gtctggaaaa gttttagcgg
    40741 tacattttgg atcaagaatc gtcacaggcg tatctgataa tgcgaccagc gagaaacaca
    40801 tcgccatccg gtgatcatta taagtggcga tctcagcaaa tttcagtttt tccggtggcg
    40861 taatgcgaat gaaatcatgc ccctcttcga cttccgcacc gactttacgc agttctgttg
    40921 ccatcgcaaa caggcgatcg gtctctttaa cacgccagtt atagatattg cgcagcgtgg
    40981 tggtgccttt tgcaaataac gccgcggtgg caatggtcat cgccgcatcg ggaatatggt
    41041 tcatatccat atcaatggcg ttcagttcac cacgcgtgca ggaaatataa tcatcgcccc
    41101 agcaaatggt cgcgcccatt ttttccagaa catcagcaaa gcgaatatca ccctgcatac
    41161 tgttgcgtcc aatgccggtc acttttacag tgccgcctct gattgctgct gctgccagga
    41221 aataagaagc cgaagatgca tcgccttcaa ccaaataagt gcccggagac tggtaagact
    41281 gccccccttt tacgacaaat tgttgatagt gctgattttc aatttcaaca ccaaacgtct
    41341 tcatcagatt gagggtgata tcgatataag gtttagaaac cagatcgcct ttaatacgaa
    41401 tcaccgtatc ttccggcgca agaggcgcag tcattaacag tgcggtgagg aactggctgg
    41461 aaacggagcc atcaacatca acgttgccgc cagtaaagcc gccctgtaaa cgcaatggcg
    41521 gataattttc ttgttccagg taagtgatct tcgccccgcc ctggcgcagc gcatccacca
    41581 gatgaccaat cgggcgttct ttcattcgcg gttcaccgct cagtacaata tcattgctac
    41641 ccaggcaaag tgctgccgcc agcggacgca ttgccgttcc ggcgttaccg aggaacaact
    41701 cccgggcact ttctgcgtgt aatggaccac cgttaccgat aatttcgcaa cgcgtacgat
    41761 cggctgaaag cgtatagctt acccctaacg ctgttaatgc attcagcata tggcgcacgt
    41821 catcgctatc cagcagattg gttaatactg ttttgccgtg tgctaatgcc gccagcaata
    41881 aagcgcggtt agaaacgctc ttggaaccgg gcagattaat agtgccatcg acacgagcga
    41941 tgggttgtaa cgttagggat tccatgaact caactctcta caacagaaat aaaaacccca
    42001 caggctggct gtggggatta tgcaaaattt cgacattagc cgtgacgacg ttcgaactca
    42061 accatgaagt ctgtcagtgc tttaacacct tccagcggca tggcgttata aatagacgca
    42121 cgcattccgc cgaccactcg gtgacctttc agcgcatgta agcccgcagc gaaagactct
    42181 tcgaggaaca atttatcaag cgcgctatcc gccaactgga acggcacgtt catccgcgaa
    42241 cggttagctt tcgccacatc attgcggtag aaatcgctgt tatcaatcac cccatacagc
    42301 agttctgctt tttgctgatt gatcttatcc attgcagcca caccgccgtt cgctttcagc
    42361 catttgaaga ccaggccaga taagtaccag gcaaatgtcg gcggcgtgtt gaacatggaa
    42421 tcgttatcgt tgaggatgga gtaatccaga atcgatgggc aggcgatatt cgctttgccc
    42481 agcaaatctt cacgaacgat gacgattgtc aggccagccg ggccgatatt tttctgcgcg
    42541 ccagcgtaaa ttacaccata acggctgaca tcaatcggac gggaaagaat ggttgaagag
    42601 aagtcagcgg cgaccaccac gtctttaccg aagtctggtg tttcatcgat agcgatacca
    42661 tcgatggttt cgttcgggca gtaatgcata taagcagcat tatcagagag ttgccattca
    42721 ctcattggct taactgcgcg aagaccatca acggtcactt tcgcatcaaa cacatttggt
    42781 gtgcagtatt ttttcgcttc tttgatagca cttgctgccc agtaacccgc atcaacataa
    42841 tctgcagtgg ttttatcacc aagaatattc aacggtactg cagcaaattg accgcgacca
    42901 ccgccatggc agaacaatac tttgtagttg gaggggacgt taagaagatc gcgaaaatcc
    42961 ttctcggctt cctctgcaac ctgaatgaac tctttgccac ggtgactcac ttccatcacc
    43021 gacgtaccaa gaccgttcca gtcgcgcagt tcctgttgag cctgtttaag cacctctgcc
    43081 ggtagcattg ccggaccaga actaaaattg aagatttgag ccatttcccc tcaccacgtt
    43141 gcgttgctat taacccgcgg tcaatcgatt gcgaccacag gtaatacatt attccagtgg
    43201 ctatcggttt tatcattcag tgacacacat cgcaatgagt aaaaccgttg agggcaaaaa
    43261 tgtggcttgc atcacacaaa agaatctctc ggattattag caaaaagccg atgtttttgc
    43321 tgtcaggtca cccgtttagc tcgacttgcc ttcgtccatt ctggatttgc acagcgcgga
    43381 cataattgtt tttcccccgc gttcatatga atgatttcac tgcaacggat gcaggcatag
    43441 tccgccgact ccggcaccac tttgtaacgc tgagtacagt cactgggtaa aataagtaac
    43501 cccggtttca ccttgtcatc ttcaaagaaa taaacactga tttgcccgtt ggtttcgaga
    43561 atcgccagac gtacctgccc caactgctct acaccgcgca atcgtagctc cataaagaac
    43621 tcaaattccg tcatgttgga gttattgagt ttcgaccagg ccagctcgcc gtcttcaata
    43681 atgacaaccg gcttgccttc cagaagatct tccagttttt cactgtgcgc catcaaccac
    43741 attaccaggc ggtataacaa cgccagagta ataaagacga taagtaccgg gaccatcggc
    43801 acatcatcat aaaacgccac atctcccgcc gctgaaccca gtgtcagaat gattaacact
    43861 tcaaacagcg acatctgccg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc
    43921 agtacaaagg tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta
    43981 tcaaatgcca tacggtggag atcgaacgcc ttcattttta tgctcttatc gtggtttatc
    44041 cgctaaaagc atagccaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa
    44101 aacccgtatt attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta
    44161 tccccggcaa agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag
    44221 acctcggttt tcagattgaa gaggcctcct ggctgaatcc cgtgcctaat gtctggtctg
    44281 tacatattcg cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaaa
    44341 aagcggcgct ggcttctgcc ctcggtgaat atttcgagcg tctctcaacc aactactttt
    44401 ttgcggactt ctggctgggc gaaaccatcg ccaacggccc attcgtgcat tatcccaacg
    44461 aaaaatggtt cccactgacc gaaaatgacg atgtaccaga agggctgctc gatgaccgtc
    44521 tgcgcgcatt ttacgatccg gagaatgaac tgaccggtag tatgctgatt gacctgcaat
    44581 ccggtaacga agatcgtggt atttgcggtc taccgtttac gcgccagtcc gacaatcaga
    44641 ccatttatat tccgatgaat atcattggta acttgtacgt ttctaacggt atgtctgctg
    44701 gcaatacccg taacgaagca cgcgttcagg ggttatccga agttttcgaa cgttacgtga
    44761 aaaaccgcat tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcac
    44821 gttatccagc agtagttgaa gcgatcgaaa cgctggaagc ggaaggtttc ccgatcttcg
    44881 cttatgacgg ttcgcttggc ggccagtatc cggtgatttg cgtggtactg ttcaatcctg
    44941 ctaacggtac ctgctttgcc tcattcggtg cgcatcctga ttttggcgtt gcactggaac
    45001 gtaccgtgac cgagttgctg caaggtcgtg gcctgaaaga tctggatgtg tttactccac
    45061 caaccttcga tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt
    45121 ccagcggttt aatctcctgg gacctgttca agcaggatgc cgattatccg tttgttgact
    45181 ggagcttctc cggcaccacg gaagaagagt tcgccacgct gatggctatc ttcaaaaagg
    45241 aagataaaga agtttatatt gccgattacg agcatctggg tgtctactct tgccgtatta
    45301 tcgtacctgg catgtccgat atttatccgg ctgaagatct gtggttggcg aataacagta
    45361 tgggcagcca tttacgtgaa acgattctgt cactgcccgg tagcgagtgg gaaaaggaag
    45421 attacctgaa cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc
    45481 gtgaactgtt gggcctggcg accgggtcgg ataacggctg gtacactctg cgtatcggtg
    45541 aattaaaagc catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg
    45601 aatggacgat ggagtttaac tcatcagtat tcagcccgga acgcgccaat tattatcgct
    45661 gcctgcaaac attgttatta ctggcgcagg aagaagatcg ccagccgctg caatatctga
    45721 atgcgtttgt tcgcatgtac ggcgcagatg ccgtggaagc cgccagtgca gcaatgagcg
    45781 gcgaagcggc gttttatggc ttgcaaccag tagatagcga tctgcacgcg tttgctgcac
    45841 atcagtcgtt gttgaaggcc tacgaaaagc tgcaacgcgc caaagcagca ttctgggcaa
    45901 aataaaatca aatagcctac gcaatgtagg cttaatgatt cgtctgagtt atattacggg
    45961 gcgttttttt aatgccccgc tttacatata tttgcattaa taaaataatt gtaattataa
    46021 ggttaaatat cggtaattcg tatttaataa atacgatcga tattgttact ttattcgcca
    46081 ggtgctccct tttaattaac tgttttagcg gaggatgcgg aaaaaattca actcatttgt
    46141 taatttttaa aatttatttt tatttggata atcaaatatt tactccgtat ttgcataaaa
    46201 accatgcgag ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct
    46261 ataatgcttt gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg
    46321 acaacccttt tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcaggtg
    46381 tctataaagc aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt
    46441 tcatctcaat cgcattcgtc ttctatatca cagcaaccac tggcacaggt acaatgccct
    46501 tcggcatggc aaaactgatt ggcggcattt gcttctctct ggggttgatt ctttgtgttg
    46561 tctgcggagc cgatctcttt acttccacag tgttgattgt tgttgccaag gcgagtgggc
    46621 gcatcacctg gggtcagttg gcgaaaaact ggctaaatgt ttattttggc aacctggtcg
    46681 gcgcactgct gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc
    46741 aatggggact aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg
    46801 ccgtctgtct tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt
    46861 ctggccgcag cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg
    46921 ccagcggttt tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc
    46981 gcgacttcgc atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc
    47041 acctgaccgt gatgaacttc atcactgata acctgattcc ggttacgatc ggtaatatta
    47101 tcggcggtgg tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc
    47161 accattaatg gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt
    47221 acatgtccga gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact
    47281 ggcagaatga agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg
    47341 acgagtcctt cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg
    47401 aaggcgttaa actggaaaac cgcactcacg cgccagttga ctttgacacc gctgttgctt
    47461 ccaccatcac ctctcacgac gctggttaca tcaacaagca gcttgagaaa atcgttggtc
    47521 tgcagactga agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg
    47581 aaggttcctg caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg
    47641 aataccgtaa aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt
    47701 gccgtaaatc cggtgttctg accggtctgc cagatgctta tggccgtggt cgtatcatcg
    47761 gtgactaccg tcgcgttgca ctgtacggta tcgactacct gatgaaagac aaacttgcac
    47821 agttcacctc tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc
    47881 tgcgcgaaga aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga
    47941 aatacggcta cgacatctct ggtccggcta ctaacgctca ggaagctatc cagtggactt
    48001 acttcggcta cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta
    48061 cctccacctt cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac
    48121 aagaagcgca ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc
    48181 gtactccgga atacgatgaa ctgttctctg gcgacccgat ctgggcaacc gaatctatcg
    48241 gtggtatggg cctcgacggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca
    48301 ccctgtacac catgggtccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac
    48361 tgccgctgaa cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt
    48421 atgagaacga tgacctgatg cgtccggact tcaacaacga tgactacgct attgcttgct
    48481 gcgtaagccc gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg
    48541 cgaaaaccat gctgtacgca atcaacggtg gcgttgacga aaaactgaaa atgcaggttg
    48601 gtccgaaatc tgagccgatc aaaggcgatg tcctgaacta tgatgaagtg atggagcgca
    48661 tggatcactt catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact
    48721 acatgcacga caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta
    48781 tccgcaccat ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa
    48841 tcaaatatgc gaaagttaaa ccgattcgtg atgaagacgg tctggctatc gacttcgaaa
    48901 tcgaaggcga atacccgcag tttggtaaca acgatccgcg tgtagatgac ctggctgttg
    48961 acctggtaga acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc
    49021 cgactcagtc tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca
    49081 ccccagacgg tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc
    49141 gtgaccagaa aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg
    49201 ctaaagatgg tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg
    49261 aagttcgtaa gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca
    49321 tcgaaggtgg tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga
    49381 tggaaaaccc ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt
    49441 tcaactcgct gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta
    49501 tgtaattaga tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag
    49561 tggggccttt tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc
    49621 caaaacagac tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat
    49681 ctggagaaac accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac
    49741 cgtagacggc ccgggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct
    49801 gtattgtcat aaccgcgaca cctgggatac gcatggcggt aaagaagtta ccgttgaaga
    49861 tttgatgaag gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac
    49921 cgcatccggc ggtgaggcaa tcctacaagc tgagtttgtt cgtgactggt tccgcgcctg
    49981 caaaaaagaa ggcattcata cctgtctgga caccaacggt tttgttcgtc gttacgatcc
    50041 ggtgattgat gaactgctgg aggtaaccga cctggtaatg ctcgatctca aacagatgaa
    50101 cgacgagatc caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa
    50161 atatctggcg aacaaaaatg tgaaggtgtg gatccgctat gttgttgtcc caggctggtc
    50221 tgacgatgac gattcagcgc atcgccttgg tgaatttacc cgtgatatgg gcaacgttga
    50281 gaaaatcgag ctcctcccct accacgaact gggcaaacac aaatgggtgg caatgggtga
    50341 agaatacaaa ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg
    50401 cattcttgag cagtacggtc ataaggtcat gttctaattt ccccgtaaag cggcaacttt
    50461 attgagttgc cgcttttttg tgcacaga
//
LOCUS       DAOFSV010000018        49007 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.018, whole genome shotgun sequence.
ACCESSION   DAOFSV010000018 DAOFSV010000000
VERSION     DAOFSV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.018"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            137..469
                     /locus_tag="QBH27_001161"
     CDS             137..469
                     /locus_tag="QBH27_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDQ7013500.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            616..1977
                     /gene="yegQ"
                     /locus_tag="QBH27_001162"
     CDS             616..1977
                     /gene="yegQ"
                     /locus_tag="QBH27_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDQ7013501.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2477..2794)
                     /locus_tag="QBH27_001163"
     CDS             complement(2477..2794)
                     /locus_tag="QBH27_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013502.1"
                     /translation="MKKIAAISLISIFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVKLKTEGAGLHGVARLTFK
                     CGE"
     gene            3209..4108
                     /gene="yegS"
                     /locus_tag="QBH27_001164"
     CDS             3209..4108
                     /gene="yegS"
                     /locus_tag="QBH27_001164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDQ7013503.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(4190..4963)
                     /locus_tag="QBH27_001165"
     CDS             complement(4190..4963)
                     /locus_tag="QBH27_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7013504.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(5069..6109)
                     /gene="gatD"
                     /locus_tag="QBH27_001166"
     CDS             complement(5069..6109)
                     /gene="gatD"
                     /locus_tag="QBH27_001166"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDQ7013505.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(6157..7511)
                     /locus_tag="QBH27_001167"
                     /pseudo
     CDS             complement(6157..7511)
                     /locus_tag="QBH27_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(7515..7799)
                     /gene="gatB"
                     /locus_tag="QBH27_001168"
     CDS             complement(7515..7799)
                     /gene="gatB"
                     /locus_tag="QBH27_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDQ7013506.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(7830..8282)
                     /gene="gatA"
                     /locus_tag="QBH27_001169"
     CDS             complement(7830..8282)
                     /gene="gatA"
                     /locus_tag="QBH27_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDQ7013507.1"
                     /translation="MTNLFVRGGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVLFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPEPQLKEYFTKYVLDSDE"
     gene            complement(8292..9554)
                     /gene="gatZ"
                     /locus_tag="QBH27_001170"
     CDS             complement(8292..9554)
                     /gene="gatZ"
                     /locus_tag="QBH27_001170"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDQ7013508.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENSSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(9583..10437)
                     /gene="gatY"
                     /locus_tag="QBH27_001171"
     CDS             complement(9583..10437)
                     /gene="gatY"
                     /locus_tag="QBH27_001171"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDQ7013509.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(10664..11716)
                     /gene="fbaB"
                     /locus_tag="QBH27_001172"
     CDS             complement(10664..11716)
                     /gene="fbaB"
                     /locus_tag="QBH27_001172"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDQ7013510.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEESSAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11973..13250
                     /locus_tag="QBH27_001173"
     CDS             11973..13250
                     /locus_tag="QBH27_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7013511.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13247..14251
                     /locus_tag="QBH27_001174"
     CDS             13247..14251
                     /locus_tag="QBH27_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDQ7013512.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            14248..15213
                     /locus_tag="QBH27_001175"
     CDS             14248..15213
                     /locus_tag="QBH27_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ7013513.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNREWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15187..15933)
                     /locus_tag="QBH27_001176"
     CDS             complement(15187..15933)
                     /locus_tag="QBH27_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ7013514.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEDVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(15985..16803)
                     /locus_tag="QBH27_001177"
     CDS             complement(15985..16803)
                     /locus_tag="QBH27_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDQ7013515.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(16868..17668)
                     /gene="thiD"
                     /locus_tag="QBH27_001178"
     CDS             complement(16868..17668)
                     /gene="thiD"
                     /locus_tag="QBH27_001178"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDQ7013516.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(17665..18453)
                     /gene="thiM"
                     /locus_tag="QBH27_001179"
     CDS             complement(17665..18453)
                     /gene="thiM"
                     /locus_tag="QBH27_001179"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDQ7013517.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTRPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMASAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(18488..18584)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18676..18948)
                     /gene="rcnR"
                     /locus_tag="QBH27_001180"
     CDS             complement(18676..18948)
                     /gene="rcnR"
                     /locus_tag="QBH27_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDQ7013518.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            19069..19893
                     /gene="rcnA"
                     /locus_tag="QBH27_001181"
     CDS             19069..19893
                     /gene="rcnA"
                     /locus_tag="QBH27_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDQ7013519.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGTTLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            20112..20450
                     /gene="rcnB"
                     /locus_tag="QBH27_001182"
     CDS             20112..20450
                     /gene="rcnB"
                     /locus_tag="QBH27_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDQ7013520.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(20532..21566)
                     /locus_tag="QBH27_001183"
                     /pseudo
     CDS             complement(20532..21566)
                     /locus_tag="QBH27_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(21582..24062)
                     /locus_tag="QBH27_001184"
     CDS             complement(21582..24062)
                     /locus_tag="QBH27_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ7013521.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELEIGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(24078..24752)
                     /locus_tag="QBH27_001185"
     CDS             complement(24078..24752)
                     /locus_tag="QBH27_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ7013522.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(24833..25375)
                     /locus_tag="QBH27_001186"
     CDS             complement(24833..25375)
                     /locus_tag="QBH27_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7013523.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKIADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLVMRVAQD"
     gene            complement(25668..25949)
                     /locus_tag="QBH27_001187"
     CDS             complement(25668..25949)
                     /locus_tag="QBH27_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDQ7013524.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(26212..27321)
                     /gene="apbC"
                     /locus_tag="QBH27_001188"
     CDS             complement(26212..27321)
                     /gene="apbC"
                     /locus_tag="QBH27_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDQ7013525.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            27453..29486
                     /gene="metG"
                     /locus_tag="QBH27_001189"
     CDS             27453..29486
                     /gene="metG"
                     /locus_tag="QBH27_001189"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDQ7013526.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            29627..33427
                     /locus_tag="QBH27_001190"
     CDS             29627..33427
                     /locus_tag="QBH27_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDQ7013527.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHVSWGKVGTNGQNQVKSFAD
                     AAAAEKAKLKLIAEKTKKGYVENASANVHITPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDSQIWQRIANNTRAYDPANNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLIIRCGMESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAITTLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDEAALTAAEKYDFPPLYRD
                     FRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWHKALVKLITITPEL
                     VCDVIPWVSAKAAGILSECRPQSVAEECEYATADMLPELFVSPPWMTKEKKKNSPVFD
                     LPVLPVPSVSDVTPEIAKKLTRTYLVTRFQQIAQQQATKQILFTDLPLMEKASWERHL
                     VPLTPEQQILWCLGFDKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFAQYHNNA
                     YKSWNLIALCYLPGQQAISFLNQIVKEDNYSGEGYILAIFGSAAIPAFMTCLQREPQR
                     LWFFTFFLGVNELALPMAQRLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKQGKDRD
                     CARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFDSEPLEEYPAKIAPLPGFYQ
                     FTLWRRPRLKSNNQPLPDDAMRHLGIMLSFPRDITPYAGLAIIKETFTRESLAEFGWD
                     LYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRTWPGESQHKRAVYGLDVLAS
                     IGSDIALMLLNGIAKKIKFVALQEHACDKINMVAENRGLTMAELEDRLAPDLGLDSSG
                     SLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKK
                     DVRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIA
                     CFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYTDYEQLPPFRQLD
                     RGYYHLADNERDSHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGDL
                     ELETEPFSLIYGETGYGDQLPVESVKITSPDNRYGKQSSLTFSALDDITASELINDIE
                     SLFD"
     gene            33440..37201
                     /locus_tag="QBH27_001191"
     CDS             33440..37201
                     /locus_tag="QBH27_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDQ7013528.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHISWGKVGTNGQSQIKSFAD
                     AAAAEKAELKLIAEKTKKGYAEEVSVTTPTSAPVQVIACSEKAPLPQDKPPFAEDHLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDSVPQENELTLLRSVAAHTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTALPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQAIRFDWNYDAHLITFTPDNKQPGYLSRFASVEMRLRKHLSLANDDVW
                     QRCADKLIAALGNIPAWHQPLVALLLPEKQDVSHEIARRLCGQKGLYALEWLKLTAAD
                     EQVLAELGKYYPGQPGQVFDDYYGGKIWCATILKEQGVGALARFAPYAAGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKTFVRFPHAALAALAELLAQKDQKRWRMMLMT
                     MLISQPTLAERVIPWLSTPAVAVLKSCQQQLTQPSNHASADMLPAVLVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARIWLLKYPEHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLH
                     LGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFV
                     RDASGSRLKDLPKPNKSDDESQANNAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEG
                     DISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAG
                     RKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            37211..40843
                     /locus_tag="QBH27_001192"
     CDS             37211..40843
                     /locus_tag="QBH27_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDQ7013529.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLCTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPGMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDETTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            40904..41220
                     /locus_tag="QBH27_001193"
                     /pseudo
     CDS             40904..41220
                     /locus_tag="QBH27_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41922..43010
                     /locus_tag="QBH27_001194"
     CDS             41922..43010
                     /locus_tag="QBH27_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDQ7013530.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            43021..45300
                     /locus_tag="QBH27_001195"
     CDS             43021..45300
                     /locus_tag="QBH27_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013531.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRKCPQEMN
                     KPELSSLADGVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTVIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLTQLPVSALQMLLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            45293..46429
                     /locus_tag="QBH27_001196"
     CDS             45293..46429
                     /locus_tag="QBH27_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ7013532.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRYSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            46426..48429
                     /locus_tag="QBH27_001197"
     CDS             46426..48429
                     /locus_tag="QBH27_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDQ7013533.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELANLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            48554..>49007
                     /locus_tag="QBH27_001198"
     CDS             48554..>49007
                     /locus_tag="QBH27_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDQ7013534.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKE"
BASE COUNT        12521 a        12510 c        11761 g        12215 t
ORIGIN      
        1 cattatccca cgcatagtca gcaaactgac atgatctctc cgccctggca ggcaccagaa
       61 aatcgctcat actttaatcg gcaaacagtc cttttagatg ctgttttcga tacgcaattt
      121 caatcaagga gtcattatgg ctggttggtt tgaactcagt aagagcagtg ataatcaatt
      181 ccggtttgtg ctaaaagcgg gcaatggtga gactatcctc accagcgagc tgtatacctc
      241 aaaagcctct gcggaaaagg gcatcgcgtc ggtacgtagc aacagcccgc aagaagaacg
      301 ctatgagaaa aaaacggcaa gcaacggcaa attctatttc aatctgaaag ccgccaatca
      361 tcaaattatc ggctccagcc agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc
      421 ctccgttaaa gcaaatggca caagccagac ggtgaaagac aatacgtaat tacgatgccg
      481 ggcgtaggtt gttggcgtcc ggcgctttac cagttacctc cacagcgcta caatgcccgc
      541 ccttaaagtg ggggcactcc cctgaccgct tcatcaggtg aagcggatct gacctgtcat
      601 cagaacgaga gaattatgtt taaaccggaa ctcctttccc cggcgggaac gctgaaaaat
      661 atgcgttacg ctttcgctta tggcgcagat gctgtttatg cgggccagcc gcgttactcc
      721 ctgcgtgtgc gcaacaacga attcaaccac gaaaatcttc aactcggcat taacgaagcc
      781 cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg
      841 aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc gctgattatg
      901 tccgatccag ggctgattat gctggtgcgt gagcacttcc ctgaaatgcc gatccatctc
      961 tcggtacagg ctaacgccgt gaactgggcg acggtgaaat tctggcagca aatgggcctg
     1021 acccgcgtga tcctctctcg cgaactgtca ctggaagaga ttgaagagat ccgcaatcag
     1081 gtgccggata tggaaatcga aatcttcgtt cacggcgcgc tgtgcatggc ctactccggt
     1141 cgctgcctgc tctctggcta tatcaacaag cgcgacccga accagggcac ctgcaccaac
     1201 gcctgccgct gggagtacaa cgtccaggaa gggaaagaag atgacgttgg caacatcgta
     1261 cacaagtacg agccgattcc ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca
     1321 accgacaaag tgtttatgat cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt
     1381 gaagatgagc acggcactta catcatgaac tcgaaagatc tgcgcgccat cgcccatgtt
     1441 gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg aaggccgtac caaatctttc
     1501 tactattgcg cacgcaccgc gcaggtttac cgtaaagcta tcgatgacgc cgctgcgggc
     1561 aaaccgttcg ataccagcct gctggaaact ctggaaggtc tggcgcatcg tggctatacc
     1621 gaaggtttcc tgcgtcgtca tactcacgac gattatcaga actacgaata cggttattca
     1681 gtttctgacc gccagcagtt tgttggtgag tttaccggtg agcgcaaggg ggacctcgcg
     1741 gcggtagcgg tgaaaaataa attctccgtt ggcgacagcc ttgagctaat gacgccgcaa
     1801 ggcaacatta actttaccct tgagcacatg gaaaacgcca aaggtgaagc tatgccggtc
     1861 gcaccaggcg atggttatac tgtgtggctc ccggtgccgc aggatcttga gctaaattac
     1921 gcgctgctga tgcgtaattt ctccggggaa accacgcgta acccccacgg taagtgatta
     1981 atttcgatta tttttcccgg atggaaaatt cttagaaacc gatcacatac agctgcattt
     2041 attaaggtta tcatccgttt cgctgaaaaa cataacccat aaaatgctag ctgtaccagg
     2101 aaccacctcc ttagcctgtg taatctccct tacacaggct tattttttat gcataagccc
     2161 tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttaa tagcacgatg
     2221 cctttcactt atcgacatcg tgctcgcaca ggttccggtt acgcacagcc agaacgcgca
     2281 tgtttgacgc ttaccaaaaa atgaacctaa agcattggaa tatttttgac atcattttct
     2341 gatggctgca taaaataaaa attctgcttt agtttcatct atctgtttgt cattattact
     2401 cacattcaat aatggtgttg aagaatatcc catcacagat aaaataaata tgtgcatggt
     2461 agtcttgaat ctattctcac tctccacatt tgaatgtcag acgagcaacg ccatgtaatc
     2521 ctgcaccttc tgtcttcaat ttaactatct gcattttttt gccctgagta acacagaaat
     2581 gggctgcatc attttttact atattttctg caccagatat tctgcccctg gctaaagaag
     2641 cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt ttatatgcaa
     2701 gaccaaattt accgatactt gtctcatcat tatgtacagc acacccagac ataagaaaaa
     2761 tactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt aaataccaac
     2821 gacacgctct ggtgtttaaa tataataatg gcatgattat tataattgaa taggattata
     2881 ataaatgttc tgtacaacat ttcctacata agtaggaatt acagacatag aggcccttcc
     2941 gggtaacccc atgggggact ttaatatatt ttgaatagta tattgaagat gccactgttt
     3001 agttgaatat taggtatatt ctctttttta aaatttatca gtggctaccg ttaatattcg
     3061 ctgtccccat tgtaaggtcc tggtggtaag cactgaatcc ccatacctga actgactttt
     3121 tatcctccga ctttcatcct gttctgactc caccttttct tttctgctct acactatcta
     3181 cagaccaatc ataaaggcac atacgatcat ggcagaattt cccgccagct tactgattct
     3241 taatggcaaa agtactgaca atctaccctt gcgcgaagca attatgctgt tgcgtgagga
     3301 aggaatgacg atccatgtgc ggatcacctg ggagaaaggc gatgctgcac gatttgtaga
     3361 ggaggcccgg aagttaggcg tcgcaacagt gattgccggt ggtggcgatg gcaccattaa
     3421 tgaagtttct acggcattga ttcaatgtga gggggatgac atacccgcgc tgggaatttt
     3481 accattagga actgccaatg attttgccac tagtgtaggg attcctgagg cactggataa
     3541 ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata gatatggcgc aggtcaacaa
     3601 acaaacctgt tttattaata tggcgacagg cgggtttggg acgcgtatta ccacagaaac
     3661 gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac atcattcatg gcttaatgcg
     3721 catggacact ctgcaaccgg accgttgtga aatccgcggt gaaaactttc actggcaagg
     3781 tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc ggtggcggtc aacaattgtg
     3841 cccgaacgca ttaattaacg atggcttgct gcaactgcgc atttttaccg gcgatgaaat
     3901 tattccgact ctcgtatcaa ccttaaaatc tgacgaagat aacccgaata ttatcgaagg
     3961 tgcttcgtcg tggtttgata tacaagcccc acacgaaatc acttttaatc ttgatggcga
     4021 accgttgagt gggcaaaact tccatattga aatacttccg gcggcgttgc gttgtcgatt
     4081 accaccagat tgtccattgt tgcgttaatc aaactattct ctgttgaacc agagagaatc
     4141 cgtaatgccg gatacggtgt aaacacctta tccggcatac agaacaatac tacaccgtaa
     4201 ttaattttac ccgtgtcttc tgcaatgcca gtttatccac ctcagacaca gcatcatcgg
     4261 taataataat gtcaattttt tcgattggca gtacctgatt aaaaccgcga cgattaaatt
     4321 tcgatgaatc aagaacggca atcactttat tcgccgctgt caccatcgca ccgctaacgg
     4381 aataaccttc attaaaggtc gtaataccat taaccgcatc aataccatca gcaccgacaa
     4441 acattaaatc ggcattaata tcctgcaggg aacgttcagc aatagatcca tgcatcgagc
     4501 gcgttttatg gcggaccgtg ccaccgcaga cgaccagagt aatgtcttta ttttcggaaa
     4561 gggcaaacgc cgccgggaga ctgttggtaa tcaccgtgat atttttggcg gtcattaatc
     4621 cttcggcgat aagcattgtg gtacttccgc tgtcgagaat aacggtcatc ccttcgtgga
     4681 tcattttgac tgccgcctgc gctattctgt ttttcggcgc gctggcgagc tgaaatcgct
     4741 ctttaaaccc gacttcctgg gtctcggttt cgctattacc tgacattatt ttcgccgcac
     4801 cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg gcacggattg
     4861 tcgcttccga ggcagcaaat actcccgcca gatcctgaac aagcacggtt ccctgttcat
     4921 tcactaattg gatgatctta ttccttcgct cgaatgaatt catcgtcatt tacccatatt
     4981 caattgtggc tagtgtaaac gaaggggaac cattggtgaa tgatctgttt tgcacaatac
     5041 cgggccagca tcacgctggc ccgcggtttc agggaatgag caacactttg cccggcatag
     5101 cattacgagc gatgtcacgc accgcctggg caaagctttc aaagcttcca cggtgagcga
     5161 ttaatggctc caggcttaac ttacgttctg tcagcaaccg gctcgccgtt tcccactcct
     5221 gccccggcca agggctggag tagttcatcc aactgccgat aaccgtcagc tctttacgca
     5281 atattttgcc aaacgttgca gatgttaaat gcagatcctg atgcaacgtg cccaccagcg
     5341 ccagttgggc atgaggaccg gcaatctcta ccgccagttc gacggtttgc gggacgccag
     5401 ccgtctcgag gataagctga ttaaagcgca gctcgcgtaa aacgccctgt atttgcggcg
     5461 cgctcatttc acggctgtta aatgtttgca tcgcaccgaa agattttgcc agtgccagtt
     5521 tttctgagct aatgtcgata gccgtcacac tctttgctcc cagcgcgaca gcgcactgaa
     5581 ttgccagcag gccaatggtt ccggcaccaa taataataac gtttttattc tcacatcctt
     5641 gcgctaaatg gaacgcatgc aggccaacgg taatcggttc aataaaagcg ccatcctcaa
     5701 taggcatatc cgcaggtagt gcaaagacat tgtttcgctt aacgacaata tattcggcaa
     5761 atccaccatc acgccgtgag ccaataaaat catattttgc gcactgggaa taaaaccctt
     5821 tcagacactc tggacaagta aaacagggta ataacggcac acaggcaacc gcatcgccgg
     5881 ggtgtaaatc atcaacaccg gatcccaccg catcaatata gccactaaat tcatggccta
     5941 acgttattgg ataataatgt gcaccatttt taaatatcct gggtaaatcg gaaccacata
     6001 agcccgagct ggcaattttt acccgcacct catcctgatg tttaatttca ggaatgacgc
     6061 tttctgctac gcgcacgata ccatcagtat cattcaccac tgatttcata aaaactcctg
     6121 attgttaagg gggataacct ccccctcagg taaaaattat tctgcgagaa cggctttctc
     6181 ttgtttaata aaaccacgcg ctctacgcca ggtcatgaaa ataccggtaa gataaattgc
     6241 gccgataaca atgaaaccgg gaatattttg cggggagaaa acctgaatca gtaaccaggt
     6301 aatgggagaa ccgccctgat ccattgaagc caccataccc ccggctttta acgcgcccgc
     6361 attagccgcc agttgggtgt gcaaaccaat agtttgcgtc gcgatccaca gggtgatgct
     6421 cataatgatg acacccgaga ttaaggtgcg gaacagattt ccacgatgca ccgcgaccgc
     6481 catcgccaca aagaagccga tggtggcaag gtcgccaaac ggcagcacct gattacccgg
     6541 cacacaaaca gcaattaaaa tggtgagtgg aataaaaatc aggcttgccg ataccaccgc
     6601 cgtatgtccc agcagcaacg ccggatcaag gccaatcagg aactcctgac cgccgaactt
     6661 cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt aaaccatcca tgatgggttt
     6721 aatcacccgt ggcatcagca gcataactgc cgccgttttt accgccagct gcaatacacc
     6781 tttgacatcg taacccgcga ggatgccgat aatcagcccc atcacaaaac ccacggtgac
     6841 cggttcgcca aaagggccaa atttgcgctg aatatcatcg gcgctaaatt taattcggtt
     6901 aacgcctggg atttctcgat gatagcatcg accagcacag caattggccc catatacgcc
     6961 gacgtaccgt gcggaatagc aatgccttcc agctcaaaga aatcacgggt atcgcgggca
     7021 aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta ccacacccgc catccctatc
     7081 atccatgaac cggttgccag atgcagcaac gcgccggtga aggtcatatg ccagatattc
     7141 cagatatcaa catttaccac ccgcgtcata cgggtcagta acatcgccac gttaaccaga
     7201 atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc aggtcatggg tgaagagccc
     7261 ggccagccga catcgaccac atgcagattc aggtcgaaat tttccgccat cgctttcgcc
     7321 gccgggccaa tggaatccag cattaagcca atcacaaggc caatgccaac aaagccaatc
     7381 ccgatatgca gacccgcttt aaagcaatcg cctgccttca tgcctaatat tttagaaaaa
     7441 ataatgatga caatcggcag catcaccgta gggccgaggt cgagaatata acgcatgact
     7501 tctgaaaaca taggtcaccc ctgtaagata gtcagaattt tattttgtaa tgcttcgata
     7561 ccgacaccag aaacaaaagg catgccgtga actaacggaa tatcgccaaa actgcgttca
     7621 actttggcgg tagtacatat caaatgcacg ccatccatat aggtttctat ttcattaacc
     7681 cgacactgga ttaattcaac aggaatatta tgactctgac acaactcttt aatttcttcc
     7741 gccgccatcg tagaggtcgc taccgcgcct ccgcaagcga caataatttt gcgtttcata
     7801 aatagtcctt tttattgtta cagagggatt tattcgtctg aatctaaaac atactttgtg
     7861 aagtattcct ttaactgggg ttcaggaaga gtgattagtg tctcgacgat atcgggctgt
     7921 tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct gctgcggatt ttccacaatc
     7981 aacgcaataa ccaacgatac cgccacgtcg ttatcatcat ccgcttgctg aaaaagaact
     8041 ttatttgttg gccttaacag ataaatggct gacgacttag catgaatcgc ctcacaatgc
     8101 ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag ggaattctgc ttctctggca
     8161 attaatgcct gtggccaggt atcatgaacc acacctttcg cgagcatctc attaccgata
     8221 tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc cacgaacaaa caggttagtc
     8281 atataccgtc cttattccgc acagccgtag cgataggcgc gcaaaacatc ataaatttta
     8341 tccataataa gctgatgcgg tattgctgat aattccccgg actgaatgcg ttcaaattgt
     8401 ttcggaagat actgactaat catgcccagt gggatgtcta cgccttccag attcaccatc
     8461 atcgtttcga cgctattttt aatccggcta tgcggccagt aataacgaat acgatccgac
     8521 aggctgtaac gaatatccag taatgaatcg ttaaaacccg tgcgataata tttcttccag
     8581 tactgcggtt cgtcgagcat cacttcttca attaccgcca ggcaaccact gctattttcg
     8641 ggggcgataa gttcctgctc aatttgcgcc agcgcaaata tcgcctcgcg taacgcaaag
     8701 gttaatgccg gaccgacttt caaaattgca aaatgatcgc gaactaattc ccgataggcc
     8761 gcctgagtct ggtaatcggt ggaatgcgct tcataaacca ttttggtttt ttctatccat
     8821 tgcgccaacg cctgagcttc ttgcgcctga taatgaataa tattgctatg gtcaaattcc
     8881 acaccaggct gcaccacgat ggcaatcacg cgtgttaacg cctctgccag accgcgggca
     8941 ataaacgctt tttgatgcgt gcgtaaagta ttggcggcat cttcaacctg ggtgatgtgt
     9001 actgactgaa tggcgctggc ctcaccgccc gggaccggaa cttcagtgcc aatgacatag
     9061 ttcagttgct cacgctggca atcagtcgcc acactttccg ccgccagaca aagcacagcg
     9121 gcacgttcag caaccgtttc tggcgctaac ggtatggaat cgtcagcgca ggacattgac
     9181 gcatcaagat gaattttact gaagccggca cgaacatatg cctttaccag ctcgacggat
     9241 ttttccatcg ccgcatccgc attttcttgc tgccagcagt ttggtcccag atggtcgccg
     9301 ccgagaataa tacgctcacg agcaaatccg actttatcgg caatagcaaa aacaaattcg
     9361 cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt gattgacctg gtttgacgtt
     9421 gcttcaatca gcactttacg cgtgctgttg cgatcaaatg ccagcgccgc ttcgataacc
     9481 aacggatgtg cagaacagac tgaacatatg ccaatatgtt caccagcttt atgccgggca
     9541 attaacgttt tcatgttttt tccttgttaa atggcaggtg cgttatgccc tgccctcgca
     9601 gccacaatcg gcaatcactt tgctcaccac atcgcgcatt gcagatttag ccgactgcaa
     9661 ataatcacgg ggatcggtcg cttcggggtg ctcggtcagg taatttttta acgcctgcga
     9721 gaaggcattt ttcagctccg ttgcaacgtt gattttgcat atccccagtt tgatggtttg
     9781 ctgaatatcc ttagtcgata atcctgacgc gccatgcagc accagcggta aattcaccca
     9841 ctggcgaatg ttctccagtc gagaaaaatc aagcgccggt gctctggcat acataccgtg
     9901 agccgtgccg atggcgaccg ccagggaatc aatcccggtt gcctcggcaa attcacgcgc
     9961 ctgaacgggg ttggtgtaca acgcatctgc ttcattgact tgcacatcat cttcctggcc
    10021 gccaagttgc cccagctccg cttcgacgct gacatcaaag cgatggcaaa aatccaccac
    10081 ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc gaggcgtcaa tcatgactga
    10141 gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat tttgtgtgat ggtcgagatg
    10201 aatcgccagt ggatggtgat attgcttcgc catcgcgctg accagcgcca tcagattttc
    10261 tgtaccggca tgagtaaatg tgccgggcgt tccggcgatg atgaccggcg catgcatgct
    10321 ggcagcggtt tccaccacca cttgcatcgt ttcgagattg tgaatattga atgccggaac
    10381 cgcataaccg ccgcgctgcg cgttgttcag catctgcttt gtcgatacca cgtacatttt
    10441 cacatcctgt catttgtttt cgatttcaaa atataatgaa attatttgtt ttcaaatgtc
    10501 gagataacga tcacaaaaac gacaatgtga aaattaatca gaatgacagg caaaaaaaac
    10561 ggcctcccgg gggaagccgt aacgaagtgc gcgtgttttt atgccggatg caacgtaaac
    10621 gccttttccg tcctacgggg tggttctgtt cgtatgaggc gcatcaggcg atagtaattt
    10681 tactatcgag ataaacgtcc tgtacggcgt taatcagttt cacgccgtca gccatcgatt
    10741 tcttgaacgc cttacgtcca agaatcagcc ccattccgcc tgcgcgtttg ttgataaccg
    10801 cagtacgcac tgcatcgctg aggtcagttt caccgcctgc agcaccgccg gagtttatca
    10861 acccggcccg gcccatatag cagttagcta actgataacg caccaggtca atcgggtttt
    10921 cactggtcag tttgctgtag actcgatcgt cggtataacc gtaattaatc gctttatagc
    10981 cgccgttatt ttccgccatt ttttgtttga cgatatctgc acctatggtt gccgccagat
    11041 ggttcgcctg accggtcagg tcggcggaaa catggtaatc aacgccatct ttcttaaagg
    11101 cggagttacg caaataggcc cacagcactg tcaccatgcc cagctcgtgc gcacgttcaa
    11161 aagccgcaga actttcttca atctggcgac gtgactcttc cgagccaaaa tagatagtcg
    11221 caccaaccgc caccgcgccc atgttgaacg cctgctccac gctggcatac agcgtttgat
    11281 cgtaggtatt cgggtaactt agcgtctcgt tgtgattaag tttgacgagg aatggaatgc
    11341 gatgcgcgta acgccgcgat accgatgcca gcacgccgta agttgacgcc acacagttac
    11401 agcccgcttc gatcgccagt tcaacaatgt ttttcgggtc aaagtagagc gggttagcgg
    11461 caaatgaagc tccggcagag tgctcaacgc cctggtcaac cggcagaata gaaagatatc
    11521 ctgtgccagc cagacgcccg gtgttgtaca acgtctgcat attacgtaac accgctggcg
    11581 ggcgattatt gtcaatcatc acgcggtcta cgtagtcatg tccggggaga taaagctggt
    11641 cagaaggaat agtcatacaa cggtgctgta aaaggttgtc ggcgtctttg ccaagcaact
    11701 gcgcaatatc tgtcattact atgctcccgt aaattccgat tggatatcgg ctatggattg
    11761 tcctggcccg ccttttgcgg gcaatcataa tcctggtcgt tacgggcgag tttttccatc
    11821 ttttggatgt tttttcagcg ttttctgttg gctcgattca tcagaaaaaa tgttaccgcg
    11881 gtcaagtttt caccgcaaag gtatttaaaa ggtattacta agtggtattg tcatcgcgta
    11941 ccttacatta cctgtcatga aggaattaaa agatgaaaac aacagcaaag ctgtcgttca
    12001 tgatgtttgt tgaatggttt atctggggcg cgtggtttgt gccattgtgg ttgtggttaa
    12061 gtaaaagcgg ttttagtgcc ggagaaattg gctggtcgta tgcctgtacc gccattgcgg
    12121 cgatcctgtc gccaattctg gttggctcca tcactgaccg ctttttctcg gcgcagaaag
    12181 tgctggcggt attgatgttc gctggcgcgg tactgatgta tttcgctgcg caacagacca
    12241 cttttgccgg gttcttcccg ttactgctgg cctactcgct aacctatatg ccgaccattg
    12301 cgctgactaa cagtatcgct tttgccaacg tgccggatgt ggagcgtgat ttcccacgca
    12361 ttcgtgtgat gggcactatc ggctggattg cctctggtct ggcatgtgga ttcttgccgc
    12421 aaatgctggg ttatgccgat atctcaccga ctaacatccc gctactgatt accgccggaa
    12481 gttctgctct gctcggtgtg tttgcgtttt tcctgcccga cacgccgcca aaaagcaccg
    12541 gcaaaatgga tattaaagtc atgctcggcc tggatgcgct gatcctgctg cgcgataaaa
    12601 acttcctcgt ctttttcttc tgttcattcc tgtttgcgat gccactggcg ttctattaca
    12661 tctttgccaa cggttatctg accgaagttg gcatgaaaaa cgccactggc tggatgacgc
    12721 tcggccagtt ctctgaaatc ttctttatgc tggcattgcc gtttttcact aaacgctttg
    12781 gtatcaaaaa ggtattgttg cttggtctgg tcaccgctgc gatccgctat ggcttcttta
    12841 tttacggtag tgcggatgaa tatttcacct acgcgttact gttcctcggt attttgctgc
    12901 acggcgtaag ttacgatttt tactacgtta ccgcttacat ctatgtcgat aaaaaagctc
    12961 ccgtgcatat gcgtaccgcc gcgcaggggc tgatcacgct ctgctgccag ggcttcggca
    13021 gtttgctcgg ctatcgtctt ggcggtgtga tgatggaaaa gatgttcgct tatcaggaac
    13081 cggtaaacgg actgactttc aactgggccg ggatgtggac tttcggcgcg gtgatgattg
    13141 ccattatcgc cgtgctgttc atgatttttt tccgcgaatc cgacaacgaa attacggcta
    13201 tcaaggtcga tgatcgcgat attgcgttga cacaagggga agttaaatga aaacagaacg
    13261 tattctcggt gctctttatg ggcaggcgtt aggggatgcg atggggatgc cctccgagct
    13321 ttggccgcgc agtcgcgtca aagcacactt tggctggatt gaccgttttc tgcctgggcc
    13381 aaaggagaat aacgcggcct gttattttaa ccgcgccgaa ttcaccgacg atacctcgat
    13441 ggcgctgtgt ctggcggatg cgttactgga acgtgaaggc aagatcgatc cggatctgat
    13501 tgggcgtaat attctcggct gggcgctgcg tttcgacgcc tttaacaaaa acgtactagg
    13561 tccgacctcg aagattgcgc ttaacgccat tcgcgacggt aaacccgttg ctgaactgga
    13621 aaataacggc gtgaccaacg gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc
    13681 ggcgcgcgat gttgattcct ttattgatga tgtggcgctg gcctccagcc cgacccataa
    13741 atccgatctg gcggttgcgg gggcggtagt catcgcatgg gcgatttctc gtgccattga
    13801 cggagaaagc tggtcagcga ttgttgattc actgccttca attgcgcgac atgctcaaca
    13861 aaaacgcatc actaccttca gcgcctcact ggcagcacgt ctggagattg cgctgaaaat
    13921 tgtgcgcaat gccgatggca ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc
    13981 aggtaccagt actattgagt ccgttccgtg cgccattgcg ctggttgaac tggcacaaac
    14041 cgacccgaac cgttgcgccg tcctgtgcgc taaccttggc ggcgacacag acaccatcgg
    14101 tgctatggcg acggcaattt gcggcgcgtt gcatggcgtt aacgctatcg atcctgcgtt
    14161 aaaggcggaa ctggatgcag taaatcagct tgatttcaac cgctatgcca cagcgctggc
    14221 gaaatatcgt caacaacggg aggcgatatg agcggcgctc gattacacac gctgctgcct
    14281 gaattaacca cgcgtcagcc agtgatggtc gtcggcgcgg cggtcattga tgtgatcgcc
    14341 gacgcttacg ctctgccctg gcgtgggtgc gatatcgaac tgaaacagca gagcgttaac
    14401 gttggcggct gcgcactgaa tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt
    14461 aatgccctgc cgctcggtca gggcgtgtgg gcggagatta ttcgcaaccg gatggcgaaa
    14521 gaggggttaa tcagcctgat cgataacgct gaaggcgata acggctggtg tctggcgctg
    14581 gttgagccgg atggcgaacg cacttttatg tcattcagtg gtgtggaaaa tcagtggaat
    14641 cgcgaatggc tggcgcgatt aaccgttgcg cctggcagcc tgctctattt ttccggttat
    14701 caactggcct cgccctgcgg cgaattgtta gtggaatggc tggaagagct gcaagacgtg
    14761 acgccattta tcgattttgg cccacgtatt ggcgatatcc cggatgcatt actggcgcgg
    14821 atcatggcct gtcgaccttt agtgtcactc aatcgtcaag aggctgagat tgccgccgaa
    14881 cgttttgctt tatccgcaga gataacaaca cttggcaagc aatggcagga gaaatttgcc
    14941 gcgccgttga tcgttcgtct cgataaagag ggcgcatggt atttcagcaa cgacgcttct
    15001 ggctgcattc cggcatttcc aacgcaagtt gtagacacca ttggggcggg cgacagtcat
    15061 gccggcggcg tacttgccgg gctggcctct ggtctgccac tggcggatgc cgtattactg
    15121 ggcaatgcag tggcgtcgtg ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc
    15181 gaggaactac tcctcgcaca caaaaacgta tagatcgctg cgacagtggc tgatgctgta
    15241 ctcaataggc cgttgctgtt ggtcaagcgc cacttgcttg atcaccagca ccggtatttt
    15301 gctatctaac tgaatatgtg actgaaactc ggcatccggc atccgggcgc taacgcggga
    15361 acgcgtacgt tgtgggtaaa tgtgctggct gcggaagtag tcataaagag aaatcccgat
    15421 ggcatcaaca tcatgaatta aatgtgccgg aacccacgat tcctcaatcg ataccgcctc
    15481 ttcatcgaca tagcgaatac gcttaagcaa aaaaacatca cttcctgcct cgaccgccag
    15541 ctgctgcgcg acgtcttcgg gacatttcac gacacgcttg ttaacccata gcgtatcggg
    15601 ctttttcccg cgcaacacca cctgctgaga aaaacctcgc gcttctttca acgagtattc
    15661 gaagatgttg ttgatctgcg tgccgtaacc acgcgaacgc gtcaccacgc cctcttcttc
    15721 cagcgactgc atcgccttgc gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc
    15781 acgctcaccg ggcaaaatat tgccatgctc cagcaccccg ctgcgcacgg cattttttac
    15841 cgtttcggca aacttaatat aaagcggcgt gttatccgcc gctaaaatac gctcgttcag
    15901 ttgggcaatc agctgggtat gcgcttgttc catttgtctt tttcctgccg gggaatctgg
    15961 tgggagtata tcactgcttc ttatttaagg cgtttcttta atcccatcaa cgaatccctg
    16021 caactcctcc tctgtgccat taaacacatt aaaatccacc ggaccattaa tgccatcgac
    16081 ctgtccacgg tcggaatgct gccagaagcg ccaggccatg ccgtcattgt ccgggcgacg
    16141 ttgataatag tgagccaccc accacggata ttcattgaaa tagcccgcca gattggtgtg
    16201 ataaaaaacg gctcctgagt aaataatcgg cttttttccc gtacgttttt cgaccatttt
    16261 tagccactga cttacccgct tgcgtaattc ttttgccgat aattttcctc gctcttctac
    16321 gtccagtacc gcagggaaat cgccttgtga gaaatccacc gtttgcagaa ataatcgcgc
    16381 ctgaactgaa gcggatactg acggggagaa ataatgatac gccccgcgca gcaggccatt
    16441 ttcccggctt agttgccagt tacgcgaaaa ataggggtcc accagctttt cgccttccgt
    16501 cgccttaata aaagcaaact gtaagcggat accgttgtcg cgcatttttg ccacccgctg
    16561 ccagtcgatc cgctcctgcc agcgcgaaac atctattccg tgaatggtgt aactggcagg
    16621 aatgcgaata gcgaaagatt taaccgggcg ataaccgtag aagtggatgt agtctttaat
    16681 ctgaattgct gtcgaataga aaaaattcac cgtctgacgc ggataaattg caacgatgga
    16741 aattagccca agagcgcata ataaaacggt gaattttttt cgactggtga ttctcaactg
    16801 cattggtttc atccctgaat gtcagcacca aaggctgaca ataaccaaag cagctatagt
    16861 acggtgctca ccaccaggcg tggaagtggt gaaccggacc aataccgtga ccaacttcca
    16921 gcgtgtcggc ctgggctaat gccgatgaaa gccagctttt tgcctcctgt acggtgtcag
    16981 cccagtttgt atggcgcggg cgcagtgcag ccaacgccgc agagagcgta caaccagtgc
    17041 cgtgagtgtt tttggtcata atgcgctgtg cggtaaaccg ttgttcaccc tcgcgggtaa
    17101 acagccagtc cgggctttgc tcatcatcca gatgaccacc tttcattagc actgcgccac
    17161 agcccatcgc caacagcgat cgcccttgtt ccagcatttc ctgttcggtg cgcgcgtgtg
    17221 gcgcgtcgag caaggcggct gcttcgggca agtttggcgt tattaatgaa acctgtggca
    17281 ataatcgact gcgtagcgtg gcaactgccg aaggtgaaag cagcgggtcg ccgctttttg
    17341 ccagcataac ggtgtcgagt accacgtttt ggatctgata acgttgcaac cgttctgcca
    17401 ccgcttcaac aatatcggtt tccgccaaca taccgatttt agtggtgtcg attcgcacat
    17461 cgctgaacac cgaatcgagc tgggcggcga caaaatcagg ctcaatgcga tacaccgact
    17521 gtacaccacg ggtattttgc gccaccagtg cagtaataac tgagcaacca taagcgccaa
    17581 gtgccgagaa ggttttaaga tcggcctgaa tccccgcacc accactcgga tcagtaccgg
    17641 caatcgtcag agcgttaatt cgtttcatgc ctgcacctcc tgcgtcaatt gccagagcgc
    17701 atcaaggaaa tgtggaacaa aactgcctgg cccctcgctt ctggcgactg cgcgttctcc
    17761 ggcttgtttc atccagtgac aggcagatgc gatattttcc agcgtatcgc ctggtagcgc
    17821 acaacaggca gcgacaaccg ccgatagtgc acagccagtt cctaccactt tggtcattaa
    17881 cggatcgcca ccgtgaatgc caatgatacg atgtccatcg gtaacataat ccacctcgcc
    17941 agtgaccacg acgattgcgc cagtttcccg tgccagtgtt tgtgcagcag gtatcgcgtt
    18001 agctgcggca tcagtggtat ccactccccg tccaccatta gcaacgccag ctgatgccat
    18061 gatttccgaa gcattaccac gtatggcggt tggcttatgg gacagaagtt ccagacaaaa
    18121 acggcggcga taatcgagcg caccaaccgc aaccggatca agcgtccagg gcgtttgtga
    18181 tcgtgttgct tgctcaacgg cagcactcat cgcttgcgct cgtggccgcg tcagcgtgcc
    18241 aacgttaatc aacaaggcac tggcgatagc cgcaaactga ctggcctctt cggtttcgat
    18301 aaccatcgct ggcgatgcgc cgagcgccag caaggtattg gcagtaaaag tttgcaccac
    18361 atcgttggtc atgcagtgca caagagggga atgttggtga aaaaggtgta acgcgtgcgc
    18421 agattgcgct gaactcagca ggtcgacttg catagtttgc tcctgccata acgtgaagaa
    18481 gcaatgacct ggtggtccgt gacttcccta cgctggcatt atccagatca ggtgatacgg
    18541 gtatttctca gccttcacaa agaagggcac cccgagtcgt ttggttgcga taataaatcg
    18601 cagaggagga tggtaatgtc cagcgcacgc gttgtaaacg gggaaaaacg gcggtattac
    18661 aataacgttg tgaaattatt tgatataaga atccagcacc ttcagaacga catccagatc
    18721 ttcttcacgt tttagctcat ccccctggtg aacgatgtgt tccgtcagat ggcctttaat
    18781 cacttcccgc atcagaccgt ttaccgcgcc acggatagca gcaatctgtt gtaaaactgc
    18841 agcgcattca tgcggttcgt cgagcatttt cttgagcgcc acgacctggc cctgaatctt
    18901 actggcacgc gctttcagtt tctgtttatc acggattgta tgagacatgg caacacctgg
    18961 ttaacaagaa tatgaaaaat catagcacta ttaatctact ggggggtagt atcaggtact
    19021 gggggggagt agaatcagat tgccgaatta atactaagaa ttattatcat gaccgaattt
    19081 acaacacttc ttcagcaagg aaacgcctgg ttcttcatcc ccagcgccat cttacttggt
    19141 gcgcttcatg gtctggaacc ggggcactca aaaacgatga tggcggcgtt tatcatcgcc
    19201 atcaaaggca ccattaaaca ggcggtgatg ctcggactgg cagcaactat ttcgcatacc
    19261 gcagtggtct ggttaattgc ctttggcggg atggtgatca gcaaacgctt tactgctcaa
    19321 tcagcagaac cgtggctcca gttgatttcc gcagtgatca ttattagcac cgcgttctgg
    19381 atgttctggc gtacctggcg cggcgaacgc aactggctgg agaatatgca cgagcatgat
    19441 catgagcatc atcaccacga tcacgaagat caccacgacc atggacatca tcaccatcac
    19501 gaacatggcg agtatcagga tgcccatgca cgagcccatg ccaatgacat taaacgacgc
    19561 tttgatggta gagaggtcac caactggcaa attttgctat ttggcttaac cggtggcctt
    19621 atcccctgcc cggcagcaat taccgtgctg ttgatttgca ttcagttgaa agccctgaca
    19681 ctgggcacaa cactggtcgt cagtttcagc ctcggcctgg cgttaacgct tgtcaccgta
    19741 ggcgttggcg cagcaatcag cgttcagcag gtcgcaaaac gctggagcgg atttaacact
    19801 ctcgctaaac gcgctcccta tttttccagt ctgttgattg gcttagttgg tgtgtatatg
    19861 ggcgtacatg gcttcatggg cataatgcga taactcaatc ctttaggctt gcatgttatc
    19921 ttcagcccag gatgattctt aaatcagcta tttccgctga ctctctgctc gctaaggatg
    19981 cttttagggc atcctttcat ttacactttt tacgaaatca tgggatcact aacaaaatat
    20041 cgcttgtcag ttatattgta tggcaggaaa gatatgcgac tgatattaca gatccccaaa
    20101 gtggagagtt tatgaccatt aaaaataaga tgttgctggg tgcgcttttg ctggttacca
    20161 gtgccgcctg ggccgcacca gccaccgcgg gttcgaccaa tacctcggga atttctaagt
    20221 atgagttaag cagtttcatt gctgacttta agcatttcaa accaggtgac accgtcccag
    20281 agatgtaccg caccgatgag tacaacatta agcagtggca gttacgtaac ctgcccgcgc
    20341 ctgatgccgg gacgcactgg acctatatgg gtggcgcgta cgtgttgatc agcgacaccg
    20401 acggtaaaat cattaaagcc tacgacggtg agatttttta tcatcgctaa aaaaaagccc
    20461 cctcggagag aggggggaat gcagacacct taatattttt attattagcc acttgctcgt
    20521 cttgcttgtt attagtcgta tttcacgttg attaatgcgg ttgcctccag tgcgccagat
    20581 ttaactttgt ttgtatcgta gacgtagtaa ctggccgtta tcggaattga aaccgttgta
    20641 tctgtaagtt ttgactttgc cgcaatttct tttaaatttg tatatccttt actgctgtta
    20701 gtgatattta aaagtgtgac tggcgtacca ttactttcaa gaataaatcc tacgccatta
    20761 tcttccgcac ctttcaggat cgtgttgctg ttggttaaca agtcagaact tgataaatac
    20821 acctgcattt tccgtgatgt ttgattttgt tgacctaata aatttttaca acgcagaata
    20881 atatcccctt ttttactatt tattgttgtt ttgttacctg tggctggtaa ttgtgaaacg
    20941 ggaatactct ctaacgtaat taataaatcg tccggtttgc aggttgtggt tatcgggttg
    21001 tatgacaatg tcagattaaa ggaatagaca cctgcatttt gtgccaatgc atttctgtag
    21061 ctatttacac atgcatccca tgacttacca cctaataaac acagtagaaa ttgtactgcg
    21121 gcactcccac gtgtcgatga tgaagcactg gataatgtcg caactccatt aatcatcact
    21181 gaaccaccgc cccccgcaga aacattcact tttgcattac tggccgcagg tgtcaccgtt
    21241 accgaataac cactggtgaa actggtctga ttatttgaca atgtaatgtt gtttttattt
    21301 gtccattcaa aatgtaaatt taatttaatg gtatcgttat aaagtccaac aataatatcc
    21361 gacgggctca taggggtgat agtatttgta ctcacgcatg acgaatcatt aaaattatgc
    21421 tcatatgtcg ccatttgtgt ttgagtaagt gcaaaatccc cctaaacaac caaattgtgc
    21481 tgaacagata actcactata acatgccgca tagctcacag gcataaccat catcattaat
    21541 ataaatagca ttatgcgaat ttccatagaa ccacctgact attactggca aatataattt
    21601 ttactttcat caatctcttt accgaaggtg attgtgcatg aaagcccttg ttgcttatca
    21661 attgccacat taaccgatgg cggtacttca ttggtgcgaa taaataactg gcttccctgg
    21721 ccgacaacac caatattatg accatggata tcattgactt cataaccaaa cgttaatggt
    21781 tgcccatccg ctcttaatgc ttttataaac catggcttgc gctgatcggt atcaaaatta
    21841 accagtacaa ccgcgccgcg ataaggggcg gcaattttcc ggttgccacg taattctgct
    21901 tcgctatcgc tttgtgacac atccaacatc aggtaatttt cccgataagg tgtcattccg
    21961 tcgtatacca ccactccatt acggtttgtt gtgcgatatt tttgcccatt gacataagca
    22021 tctttaattc ctggcgcatt catcacagca aacgtttcag aaagacggtt tgccagatta
    22081 acgccacccg accaggcgac aatgccccct gaaacactgg ctccagcctg tcgataagca
    22141 ctcgactgac tataactgcc attcactgtc gcaaccggcg cgttccaggt taaattcgcc
    22201 ccagctgtcg tttcatttcc ctgatactga taactcaggt tgaccccata gttaaactga
    22261 tcccggcttc caacggttcc tgataatccc gtattatttg aggcaacccc ctgatcatca
    22321 aacgtcgttg agttagacat atatatttgc cgacgaggcg tcgtaacgtc atcaccccaa
    22381 tcaaagggaa tcgatataaa aatattaaaa cgtttctctt catgatgatt ctcgtcataa
    22441 gcatggcttg ccgcgagggt atagcttatc cttcgcaggt tgttggaata actcaactga
    22501 taatccttac tgctgccgct acgcccccag taatctcgcc ataacgtact taaggacaca
    22561 gaaccccagc cttctggcaa tgactgactc atattggcag aaaagctatt tttacggcca
    22621 aaatcgttct ggtaataatc ggcaatgtca tagatatcgt tttcatcacg gcgataatta
    22681 tctttattgt ttgcccatac gtgatcatta aatgtccgat aatcacgcga cgaataacgc
    22741 caggccgcca gaccaaaacg tgtcgacgtt tggctcacaa atttgttgta ggcaatttga
    22801 taactttgcc cgtcaaacac atcgccgttg tcttgtttac tatgcgactt tgtggcatcg
    22861 acggaaatgg caccaaagcg tgtattccag ccagtcccca aagtgaacgc gtaataatta
    22921 ttcgcgacca tcgagccacc atacagcgtt aataagttat taaaaccata ctgataaccc
    22981 gcctggacaa aatcactttg tttgctcgcc ccttcaatat ggctacgacc cgccgcaaaa
    23041 tcatatttcg acacgccggg ttgcagcata tttggtaccg ctgcataagg caccagatag
    23101 gtggttaccg aaccgtccgc ctctttcacg ctaacatcaa gatccgctcc accaccggcc
    23161 aactgcaaat ctgtaatcgc aaacgggcct ggaggaacct ctttctgata aaccacaaaa
    23221 ccattctgtt caatagttac cagcgcgtta ctctgagcaa tcccctgcac tcgtggcgta
    23281 aaattttgtt tcgagttagg caacatctgc atatcacgaa acaaccgcac accgctgaag
    23341 cgaacagaat caaaaatatc gcttgatgtg tacatatcac ccacacgaag cgtgccgaga
    23401 agttgggcaa atccacgttc cagatacagg gtattgctct tccacacccc tggattgtta
    23461 tttgttttac tgaaactggc atcagaatgc agttgccacc ccaataaatt taacccgctg
    23521 ttaaaacgta catatgtact cttgctatta ccggacgctt tataatcgct gtaatactga
    23581 ctcatataat aagaggtata aaacgcatta ataccccgct cccagttttc aggtggtaca
    23641 tagccaattt ccagttcttc cacccaggct tgcgggacac tgaaatcgag acgaaaaact
    23701 ccgatatccc aggtatagct cccgccctga ataagttgct taaatgttaa acattgctta
    23761 ccgctggcaa agttatcggt attaatgcct aaccgcttga tcatttccct tgataaacat
    23821 gtttcttgcg ggttgtcttt aacaatgatc tcatatttcc cacgccattg cttattgaca
    23881 taaatatcga tgtcatactg ccccggcaag ggttgattat catcaagacg aatatttgca
    23941 acctgctggt ctttcattcc acctatcatg aaatgggtat caaaggtttc ttcagctgca
    24001 taagcttcaa tgccgatcaa taacgctacg attgctgatg caagtggggt cattctcaac
    24061 ataggcagct cctgcaatta aattttgtca ctaatatagt tgccatggtc atcgataatg
    24121 gtcagatgcc agttatttgc attattactt ttgacattaa cactctgact ttctaagggg
    24181 gcaatcataa tagtttcata attgactttg acattattag ctttgacatc cgaaatcgtc
    24241 acccaattag ctgagtcatt ttttatcacc aatccattgc cactgcgatt taccagtaac
    24301 tttttaaagg tcgctttatt taccggagca atacccgctg gccggtaaaa caacttaatt
    24361 ctgttttgca tcgcaaactt cagtgcattc ttaccttctt gttctggact atttggtgga
    24421 atatccagaa cattcagata aaaaatactc tctttattag tgggtagttt attcggcata
    24481 attttaattt ttacttgctg cccggaattt gcccctattt ttgccactgg tggcgttaac
    24541 atgaaaggaa cctgaatttt ttctggtggt aatgacgtat cgccatcatc aatccacgcc
    24601 tgcatcagcg aagaacgatt tccctggttc atcagctgca ccatcacttc tttgtgctct
    24661 gccgggtaaa taatacgcgt cccgtagata acaataccgg catgtgcagg aaggaccata
    24721 gaagaaatga gtaaagctaa taatcctttc atcttaatgc ctcttaaatt aaaaggccgc
    24781 catggggata gcggccatgt taattacctg aagaatcttg caattttatt tattaatcct
    24841 gcgccacacg cataaccagg gaagataaca catcacctga attcactgag ttatctgtca
    24901 gtttcaggta ttttgcttta aacggaatgg catattgacc gtccgctatt ttataactct
    24961 cgatgttgaa aggtgcagaa acatccagga catcgctgtt atcacctaaa tagagataat
    25021 aacccacggt atcgttgcta ctggctatcg ccttatcgtt tccggttatt ttatcgttgg
    25081 taatcaacga gagttttaca ccagcaactt ctttcggaca gctaattttt aacatgctgc
    25141 tggttacacc gactgcatca tcataggtcg agtatttagc tttaccttca aagacagtgg
    25201 ttggtacagt tggcatatta atacttactg aatcgccacc cgcttcgaat ttacacggag
    25261 attctgcaat attcccttta atggttaatg ttccggtatc aacgtctgcg gcaaataccc
    25321 cagcgctcat aaaaaaagaa gaaaaaacag cggcagcaat aattgaacgt ttcataatat
    25381 gctccttaat aaaatctatt tttggttgtt aagagtctgt atctcttgga aacaaatttc
    25441 tcacaccaaa aaagttgccg caatgtaaac acattgtttt attgatttaa agcaaattaa
    25501 ctattgcaat ataaatacaa caaatactta agcacattaa atatttatca aatctattaa
    25561 ttaatgtgga tttctataaa ttgtattatt aatatcaata aaaaagctta aaccattcat
    25621 gtaaatgatt taagctttct aaatccgatt aaaaccaggg tggcaaatta atcgtagcga
    25681 tttaacacga ttcgacgttt gctatcgcta tctgcagcaa tcacttccgt cgttacacct
    25741 ttaactggcg aagaaacctg atgacgggta tcaacattat acgttaactg accacaatgc
    25801 tgttgaggtt gaccattaac catagccata ctgcatgtcg ggggactgat cctaccatta
    25861 atcgttaatg tggcagtttc tgaagagaac gcgggagaag ccaatccata ggcaagaaaa
    25921 ataattcctg acagccagta cttattcatt gaacgttatc cctgtagtaa aggttatgcc
    25981 tggaaagaat gagtgcataa caaactatag ctgtacatcc actacacagc cacgaaggat
    26041 gataatgaag catcgccggt acgatcaatc gactttgtag agtttcggac gaaggtccgc
    26101 agaatattcg caacattaaa taagtgttca ttaatgttgc aaaaacaaat acaccgcatc
    26161 aatgtaacat ctctgcggtg tatgaagaat aaccagaatg gttaatacgc gttagaccgc
    26221 gcggaaggaa atctcgccag gaatgacttc accctgccag tagagctgag ctgcaacgcg
    26281 gtcagccagc tggcggtaga tagcggtaaa ttcgctctca ggacggctga taaccgttgg
    26341 cgtgccttta tcgagatcct cacgtaagga gatgtgtaac ggcatctgcc ccagcagctg
    26401 cgtgtggtat ttctcggcca gtttctctgc accaccggta ccgaaaatcg gctcatggtg
    26461 accacagtta ctgcaaatat gcacactcat gttctcgaca ataccgagca ccggaacctc
    26521 gactttctcg aacatcacaa tgcctttttt cgcatcgatc aacgcgatgt cctgcggggt
    26581 ggtgaccact accgcgccag ttactggaat gttttgtgcc agtgtcaatt gaatgtcacc
    26641 ggtacctggc ggcatatcaa gcaccagata gtcgagatcc ggccacaagg tttcctgcaa
    26701 catttgcatt aacgccttgc tcgccattgg gccacgccac accatcgcat tgtcgtcagt
    26761 gaccagataa ccaattgaat tggttgccag gccatgagac ataatcggtg ccatatgggt
    26821 accgtccggc gaagttggac gctgattttc tgcgcccagc atggtaggga ttgatgggcc
    26881 gtagatatcg gcgtccagaa tgccgacttt cgccccttct gccgccagtg ccaacgccag
    26941 gtttaccgcc gtggaggatt tacccacgcc acctttaccg gaactgaccg cgataatgtt
    27001 cttcacgccg ttaattcccg gctgattctt cacgcgtttc agagtggcta tgttatgcga
    27061 cagtttccag tcgatagctt tcgctccggt gatgcgcagc agttcggcgc tacattgctc
    27121 ttttaactct tcgaacgcgc tatgccagac gaatggcatg accagttcca catgcagcgt
    27181 gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtt gtgaggttat gcttcagggt
    27241 tgggtgctga aaattggcca gtgtcccggc gaccattgcg cgcaaggctt ctggtgattt
    27301 ggcctgggat tgttcgttca tcccgactcc ttttttgtat agataaacca tcagctgata
    27361 gtttacctga agaatataga gaagtactta cttaacattt tcccatttgg tactatctaa
    27421 gcccttttca ctattaagaa gtaatgccta ctatgactca agtcgcgaag aaaattctgg
    27481 tgacgtgcgc actgccgtac gctaacggct caatccacct cggccatatg ctggagcaca
    27541 tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg ccacgaggtc aacttcatct
    27601 gcgccgacga tgcccacggc acgccgatca tgcttaaagc tcagcagctt ggtatcaccc
    27661 cggagcagat gattggcgaa atgagtcagg aacatcagac tgatttcgca ggctttaaca
    27721 tcagctatga caactatcac tcgacgcaca gcgaagagaa tcgccagttg tcagaactta
    27781 tctactctcg tctgaaagaa aacggtttta ttaaaaaccg caccatctct cagctgtacg
    27841 atccggaaaa aggcatgttc ctgccggacc gttttgtaaa aggcacctgc ccgaaatgta
    27901 aatccccgga tcaatacggc gataactgcg aagtctgcgg cgcgacctac agcccgactg
    27961 aactgatcga gccgaaatcg gtggtttctg gtgctacccc ggtaatgcgt gattctgaac
    28021 acttcttctt tgatctgccc tctttcagcg aaatgctgca ggcatggacc cgcagcggtg
    28081 cgttgcagga gcaggtggca aataaaatgc aggagtggtt tgaatctggc ctgcaacagt
    28141 gggatatctc ccgcgacgcg ccttatttcg gttttgaaat tccgaacgcg ccgggcaaat
    28201 atttctatgt ctggctggac gcaccgattg gctatatggg ctctttcaag aatctgtgtg
    28261 acaagcgcgg cgacagcgta agcttcgatg aatactggaa gaaagattcc actgccgagc
    28321 tgtatcactt catcggtaaa gatatcgttt acttccacag cctgttctgg cctgccatgc
    28381 tggaaggcag caacttccgc aagccgacca acctgtttgt tcacggctat gtgacggtga
    28441 acggcgcgaa aatgtccaag tctcgcggca cctttattaa agccagcacc tggctgaatc
    28501 attttgacgc cgacagcctg cgctactact acaccgccaa actgtcatcg cgcattgatg
    28561 atatcgatct caacctggaa gatttcgttc agcgcgtgaa tgccgatatc gtgaacaaag
    28621 tggttaacct ggcgtcccgt aacgctggct ttatcaataa gcgttttgac ggtgtgctgg
    28681 caagcgagct ggctgacccg cagttgtaca aaaccttcac tgatgccgct gaagtgattg
    28741 gcgaagcgtg ggaaagccgt gaatttggta aagctatccg tgaaatcatg gcgctggctg
    28801 acctggctaa ccgctatgtc gatgaacagg ctccgtgggt ggtggcgaag caggaaggtc
    28861 gcgatgccga tctgcaggcg atttgctcaa tgggcatcaa cctgttccgc gtgctgatga
    28921 cttacctgaa accggtactg ccgaaactga ccgagcgcgc agaagcattc ctcaatacag
    28981 aactgacctg ggatggtatc cagcaaccgc tgttgggcca taaagtgaat ccattcaagg
    29041 cgctgtataa ccgcatcgat atgaaacagg ttgaagcact ggtggaagca tccaaagagg
    29101 aagtaaaagc cgctgccgcg ccagtaactg gcccactggc agatgatccg attcaggaaa
    29161 ccatcacctt tgacgacttt gctaaagttg acctgcgcgt ggcgctgatt gaaaacgcag
    29221 agtttgttga aggttctgac aaactgctgc gcctgacgct ggatctcggc ggtgaaaaac
    29281 gcaatgtctt ctccggcatt cgttctgctt acccggatcc gcaagcacta attggtcgtc
    29341 acaccattat ggtggctaac ctggcaccac gtaaaatgcg cttcggtatc tccgaaggca
    29401 tggtaatggc tgctggtcct ggtgggaaag atatcttcct gctaagcccg gatgccggtg
    29461 ctaaaccggg tcatcaggtg aaataatccc ccttcaaggc gctgcatcga cagcgccttt
    29521 tctttataaa ttcctaaagt tgttctcttg cgattttgtc tctctctaac ccgcataaat
    29581 actggtagca tctgcattca actggataaa attacaggga tgcagaatga gacactttat
    29641 ctatcaggac gaaaaatcac ataaattctg ggcggtggag cagcaaggca acgagttaca
    29701 tgtcagttgg ggcaaagtcg gcactaacgg gcaaaaccag gtaaaaagct ttgctgatgc
    29761 tgcggcggcg gaaaaagcaa agcttaagct gatcgcagag aaaacgaaaa aaggctatgt
    29821 ggaaaatgct tctgcaaacg tgcatatcac ccccatcacc aaagccactc ctgaagttga
    29881 aacttctcct gagagtaaaa accaacgccc ctggctggct gatgatgcgg tcattcggct
    29941 aactgacgat attaatcgat ttgcttttcc tcatcgctct cgccccagag agatcaatta
    30001 tctgcacaaa gatagtcaaa tatggcagcg catcgcgaat aacaccaggg catatgatcc
    30061 cgccaacaat taccgttctt atccagaaaa ctggcagcag gcttttgctg agttacaaag
    30121 gcgaattctg gataatcaac agacagggag tgcgcaatct gatgcggcat tgctctggag
    30181 tttctggaat tcttactctg cagatgaact agtcgatgac ttaatcattc gctgtggtat
    30241 ggaaagtgcc gttgaaattg cccttctcgc tcttcaactg agatataaac cagtaaaagg
    30301 cgcagtaacc accatcattc cacctaatca cgaagcagaa tcgctaccga gttggcatca
    30361 acgcctatgt catcatcttt cactcgcttc agaagatgag tggcaacgct gtgtagataa
    30421 agtactcgcg gctattacga cgctatcccc agcacggcaa ccttttgcgg cgttactgct
    30481 ccctgaacgc ccggatattg ccaatgctat ggctctacgc tatgcagatc aaaacgttcc
    30541 ggcgatgacc tggttaagac tggtagtaac cgatgaagcc gctctgactg cagccgaaaa
    30601 atacgacttt ccaccgttat atcgtgattt tcgcgcttat ctggcaatgt tgctggcaaa
    30661 taatggggtg agcggtgtaa gccggatact tactgagttt gtggaagagc attctgacaa
    30721 tcccacttat ctcttcgagc gcatcagcga gactgaagat cttgtcaaat ggttatggaa
    30781 aacgaatcac ccggatgcga ttcaaattct gatcctcggt gtaattggca agaaaaagca
    30841 cctggaatac ttaagcaaag catgtcaaaa acatcccgct gcggctattg ccgcttatgc
    30901 aactttgctg gcaatacatg aagataaaga gtggcataaa gcgcttgtca aattgattac
    30961 catcacgcca gagttagtat gcgacgtcat tccctgggtt agtgctaaag cggcaggcat
    31021 cttatctgaa tgtcgtcctc agtcagtagc tgaagaatgt gagtatgcca ctgcggatat
    31081 gttgccagag ctgtttgttt cgccgccctg gatgactaaa gagaaaaaga aaaattcgcc
    31141 ggtgtttgat cttcctgtac tgccagttcc ttctgtttct gacgtcactc cagagattgc
    31201 caaaaaacta acccgtacat accttgtcac ccgcttccag caaatagccc aacagcaggc
    31261 aacaaaacaa atattgttta ccgacctgcc gctgatggaa aaagcaagct gggagagaca
    31321 cttagtcccc ctcacaccag aacagcaaat tctgtggtgt ctgggttttg acaaatggcg
    31381 ggagagcgga gaaaaaatat acgaaaaaat tccggcgcca caaagtgccg ttgatgccct
    31441 gctacgcttc gattttccag cactcaatgc tgaatttgcg caataccata acaatgctta
    31501 caaaagctgg aatctcattg ccctctgtta tctacctggt caacaggcca tttcttttct
    31561 taaccagata gtcaaagaag ataactactc tggtgaggga tatattctgg ccatttttgg
    31621 tagcgccgcc attcctgcat ttatgacctg cctgcaacgt gagccacaac ggttgtggtt
    31681 tttcaccttc ttccttggtg tcaatgaact ggcattgcca atggcgcaac gacttcagaa
    31741 aaaaatgtcg tatgaagatg cacgaaactg gctaacggat tacccacgcc atgccgccgc
    31801 cgggctactt cctgtggcac tgggcaaaca aggaaaagat cgtgattgtg cccgccaggc
    31861 gctccgtctg ctggtgaatt taaaccagcg agaaacgatt gaggaaatcg cccaaggata
    31921 caaccaacct gatgttttag ctgcgctggc aacattattt gatagcgagc cgctggaaga
    31981 atatcccgct aaaatcgccc cactgcccgg cttttatcag ttcaccttat ggcgcagacc
    32041 acggcttaaa agtaacaacc agcctctgcc agatgacgct atgcgccacc tcggcataat
    32101 gctgagcttt ccacgcgaca ttacccccta cgcagggctt gcgatcatca aagaaacctt
    32161 cacccgcgaa tcactggctg aatttggctg ggatctgtat accgcctgga cagaagctgg
    32221 cgcacccgca aaagaaaact gggcatttac ctcgctgggg attttaggca atgacgacac
    32281 cgcgcgtaaa ttaaccccgc tcatccgcac ctggccgggt gaatcccagc ataaacgcgc
    32341 ggtttacggg ctggatgtgt tagccagtat cggtagcgac attgcgctaa tgttgcttaa
    32401 tggcattgcg aaaaagatta aattcgttgc attacaggaa catgcctgcg acaaaatcaa
    32461 catggtcgct gaaaatcgcg ggctgactat ggctgaactg gaagaccgcc tggcaccgga
    32521 tttaggactt gatagcagtg gctcgttgac actggatttc ggcccacgtc aatttaccgt
    32581 tggttttgac gaaactttaa aacccgtggt tcgcgacgca aacggcaaag ttctgaaaga
    32641 tttacccaaa ccaaatcaga gcgatgataa aacgctggca actgacgcgg ttaatctctt
    32701 caaacagttg aaaaaagatg tacgcgccat agccagccag cagattgatc gtctggagca
    32761 ggctatgtgc cagcgcagac gctggacggc agagcagttc cgcctgttcc tggtggagca
    32821 tccgctggta cgtcacttaa cccgacggtt gttatggggg gtatatactg aggaaaacac
    32881 gctgattgcc tgcttccggg tcgcagaaga cagcacttac agcgatgcgc aggatgagct
    32941 atttacgttg ccagcaggaa acatcggtat tccgcatgtg ctggaaatat ctcctgaatc
    33001 cgccgctgca tttgggcaaa tttatactga ttacgaacag cttcctccgt tccgccaact
    33061 cgatcgtggt tattaccacc ttgctgataa tgaacgtgat tcccatgaat tgatacgctg
    33121 gcaaggacgt ctttgccagg ccgggcgcat tgtcgggctg gaacgccgag gctggcaacg
    33181 tctggaagaa agcggcagcg tttacgcgat gcgcaaatct actccttatg gcgatctcga
    33241 acttgaaaca gaaccttttt cattaattta tggtgaaacg gggtatggcg accagctacc
    33301 ggttgaaagc gtaaaaatca cctcgcctga taatcgctac ggtaaacaat cctcactcac
    33361 tttttccgcg ctcgacgaca tcaccgccag cgaactcatt aacgacattg aatcactgtt
    33421 tgattaaaag gatttagcaa tgaaaaccta tatttatcag gacgaaaaat cacataaatt
    33481 ctgggcagta gagcagcagg aaaatgaact gcatatcagt tggggcaaag tcggcactaa
    33541 cgggcaaagc cagataaaaa gctttgctga tgcggcggcg gcggaaaaag cggagctgaa
    33601 gttgattgcg gagaaaacga aaaagggcta cgcagaagaa gtatccgtga cgacaccgac
    33661 atctgccccc gtccaggtta tcgcatgctc tgaaaaagcc cctttgccgc aagacaaacc
    33721 accatttgct gaagaccacc ttccctggct ggcagacgac gcccaaatca ttttaccgac
    33781 agaggtcgca ccaaccacac taagtcatcg acgctggcct ggcgattctg tgccacagga
    33841 gaatgaatta actctgctac gctccgtcgc cgcgcatact caccggcgtt ttaaaaaggt
    33901 gatcaccttc gattacagca cctgttcact ggactggcag caggcgatta cacaagcagt
    33961 gggccttatc gactcaccga ttagcaccgc actaccaccg atggttctgg cggtactggt
    34021 tgcgctggaa caaggcttca atcgcaacga tcacgaagag ttaatggatc aaattgtcca
    34081 ggagggtggt ctggaatatg ccactgaggt tgtcattgca ttgcaagcca tccgctttga
    34141 ctggaattat gatgcccacc tcattacgtt taccccggat aacaagcagc ctggctatct
    34201 gtcacgcttt gcctcggttg aaatgcgtct gcgtaaacat ctctcactag caaatgacga
    34261 tgtatggcag cgctgcgccg acaaactgat tgcagctctc ggaaacatac ccgcctggca
    34321 tcagccgctg gttgcgttat tgttgccgga aaaacaagat gtgagccatg agattgccag
    34381 acgtctttgc ggacaaaaag ggttatatgc gctggaatgg ctaaaactaa cggccgccga
    34441 cgagcaggta ttggcagaac tggggaaata ctatccagga cagccagggc aggtatttga
    34501 tgattattac ggcggtaaaa tctggtgcgc aacgatactc aaggagcaag gtgtcggtgc
    34561 gctggcccgt tttgctcctt acgctgctgg tgacacatgt ggtgaagtgt tgatgcatat
    34621 caaccacccg caggcactta cactacttat ccatgcaagc gagcagggaa agcgttgcca
    34681 cgatcgcatg acaaagactt tcgttcgctt tccacatgca gcgctggcgg cattagcaga
    34741 acttctggca caaaaagatc aaaagcgttg gcgcatgatg ctaatgacaa tgcttatctc
    34801 acaaccaaca ttagcagagc gagtaatccc ctggctctca acacccgcag ttgccgtact
    34861 gaaatcatgc cagcaacaac tgacacagcc ctcaaaccat gccagcgctg atatgctgcc
    34921 tgccgtatta gtctcaccgc cctggctttc gaaaaagaaa aaatctgtaa tgccggtatt
    34981 ggatttaacg ccattaccgc tggagtcatg ctgcacattg accgagacgg cagaaaaaga
    35041 gattcatgca aggcatcgct ggcacgcaca tcaaatagac atcggccaaa aagaggatat
    35101 tcagaactac ctgacacgtc tgggtttcaa tcgctggaat aatggccagt atatgaaagc
    35161 gtctgatgct gttgtagaac tctggcagag gggagattat tctgcgctaa tcagcgaatt
    35221 taaaaccttc tggcactcct atcaacggga atggcagctt tacatgttag ccgccctgcc
    35281 tatcgaaaaa acagcgcagg cgtggaatgt cctcagtaaa gagccacacg tcggtgtcga
    35341 gtttgtcatg acgcacctgc aactcgctgg gttacagggt tttattcact ccttttcacg
    35401 ctatccacaa gaagctctgc ctgtagcaca atattttgcg gctatcgaat tagcgccact
    35461 gatcgcccgc gccttcaaca aactcaaaac attacgacaa gacgcccgta tctggctgct
    35521 gaaatacccg gaacacgcca ttaccggcct gctgcctgca gcgctcggca aaaccggtga
    35581 agcacaggac aacgcccgtg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
    35641 gctgcaagaa atcgcccggc gttataacca gccggaagta accgatgcgg tgaacgctct
    35701 acttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
    35761 tcagccatcg ctctggacgc gcccgttatt aaaagcaaac gcccaatcac tgcctgatag
    35821 cgcactcctc cacctcggtg aaatgctccg cttccctcag gaagaggcac tgtatccagg
    35881 attattgcag gtgaaagatg cctgtaccac cgactcactg gctgaatttg cctgggatct
    35941 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
    36001 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatcc gtgcctggcc
    36061 tggcgaatcc cagcataaac gtgccaccgt tgggttggat atcctcgccg ctatcggtag
    36121 tgatatcgcc ctgatgcagc ttaacggcat cgcccagaaa ctgaaattca aagcattaca
    36181 ggagcgggca aaagaaaaaa tagccaacat tgccgagagc cgcgaactca cggtggcgga
    36241 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
    36301 tttcggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
    36361 cgcttccggc agtcgtctga aagacctgcc caaaccaaac aaaagcgatg atgaatcaca
    36421 ggcgaacaat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgccgc
    36481 ccagcaggta gcaaggctgg aatccgccat gtgcctgcgt cgccgctggt caccggaaaa
    36541 ctttcagctc ttcctggttg agcatccgct ggtccgccac ttaacccgcc gtctgatttg
    36601 gggcgtttat agcgccgaaa accagctact ggcttgcttt cgcgtagcgg aagataacag
    36661 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctattggtat
    36721 tcctcacgtt ctggaaatat caccgacgga tgctgccgcc ttcgggcagc tttttgccga
    36781 ctacgaactg ttaccaccgt tccgccagct tgatcgtaac agttacgcgc tgacagaagc
    36841 cgagcgcaat gccagcgaac tgacacgctg ggcaggcaga aaatgcccga gtggtcgggt
    36901 aatggggctg gcaaataaag gctggatgcg tggtgaaccg caggacggtg gctggatcgg
    36961 ctggatgatc aaacctctgg gccgctggtc gttaatcatg gaaatcgatg aaggttttgc
    37021 ggtcggcatg tcgccagccg aactcagtgc cgagcaactc ttaagcaagc tgtggctatg
    37081 ggaaggcaaa gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc
    37141 cgtactcgat gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata
    37201 aggaaagagc atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa
    37261 atataaaaaa ggcaaaacac cactcagcca tcgcaattgg ccaggcgaac cggtacctgt
    37321 tatcactgaa aacatcattc agacactggg tgatgaattg ctacaaaaag cagagaagaa
    37381 aaaaaacatt gtctggcgtt atgaaaactt ttcactggag tggcagtccg ccatcacgca
    37441 ggccatcaac ttgatcggcg aacacaaacc ctcaatcacg gcccggacaa tggcggcgct
    37501 agcctgtatc gcgcaaaatg atagccaaca gttgctcgac gaaatcgtcc aacaagaggg
    37561 gctggaatat gcaactgagg tggtgattgc acgccagttt attgtacggt gttatgagag
    37621 tgatcctctg gtagtaacat tgcaatatca gaacgaggac tatggctatg gttatcgctc
    37681 agaaacctat aacgaattcg atctccgatt gcgtaagcat ctctctctgg cagaggaaag
    37741 ctgctggcag cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg
    37801 ccgccctttt attgcgctga acctcccgga aaaacccgaa atcgccaacg agctggtaag
    37861 ccttgaatgt tcgcaaactc attttcgctc aaaagagtgg ttaaaagttg ttgctgatga
    37921 ccctaaggcg gtgaaagaac tcgcacgcta ctggagccag gatatattta gcgatcgaga
    37981 agccagctac atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg
    38041 cgaacaagga ctggcagccg ttccgcgcct cgcgatgtat gcacataaag aagattgcgg
    38101 cagtctgctg gtacaaatta accatccgca agtcatccgc accttgctac tggtggctga
    38161 taaaaacaaa cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac
    38221 gctcgccgca ctggcagagc tgctggcgtt aaaagccccc cctgcgcgcc ctggttatcc
    38281 catcatcgaa gacaaaaagc tgcctgcaca gcaaaaagca cgtgatgaat actggcatac
    38341 gctgttacaa acgctgatgg catcgcagcc acaactggca gaagaggtga tgccgtgctt
    38401 atgtacacaa gcccaggcag tggtgaatgg ttatttatcg gcatcgccaa aacttgcttt
    38461 cgagagtact catagcaacg acctgccaga aatacttgtt tctccaccgt ggcgaggtaa
    38521 gaaaaaaacg gcattgctgc gccttgattt agtgcctctt gaattagcgc ccaaagcccg
    38581 ctggcaacca ggcgaacgag agagacttgc cgccacagag tctgcccgtt atttcagcac
    38641 gggatctttt acggaacgca tggaacgaaa aagtggtcga gttgtactac aggagctagg
    38701 ttttggggat gatgtatggc tgttccggaa ttatatactg cccggcaaac tggatgcggc
    38761 gcgcaaatca ttagtgggtc agtggcatta ctccccgcgc agagtagagg aaatcaacaa
    38821 tggctggcac tccacggaag caaaatcagc cgaacaggcg cttcgttccg gcgacgttga
    38881 agcgttaatt aacacctggg aaaatgatag ctactcgcat tatcgtcagg aaaagagtgt
    38941 ctggaacctg tatttattgg cgcagttgcc acgtgagatg gctttgactt tctggctgcg
    39001 tatcaatgag aaaaagcatc tattcgcggg tgaggactat tttctcagta tcctcgggtt
    39061 ggatacatta cctggcctgt tattggcttt ttcacatcat ccaaaagaaa catttccgtt
    39121 aattttaaat ttcggcgcaa cagaactggc cctgcccgtt gcccgcgtct ggcgacgttt
    39181 cgcggcgcag cgtgatctgg ctcgccagtg gattttacaa tggccggaac atacggctac
    39241 tgcacttatc cctcttgtct ttacaaaacc cagcgataat agcgaagctg cattacttgc
    39301 cctgcgttta ctgtatgaac aagggcatgg cgaattactg caaaccgtgg caaaccgctg
    39361 gcagcgtaca gatgtatggc ctgccctgga gcaattactt aaactgggtc caatagaaat
    39421 ttacccggca cgtattccaa aagctcctga tttctggcat ccgggaatgt ggtccaggcc
    39481 gcgccttatc actaataatc agcctgttac cgatgacgct ctggaaatta tcggcgaaat
    39541 gctgcgcttt acccagggcg gacgttttta tggcggactg gaacagctga aaacgttttg
    39601 ccagccgcaa acgctggcag cttttgcctg ggatcttttc accgcgtggc aacaagctgg
    39661 tgcccccgca aaagacaact gggcatttct ggcgttaagt ctctttggtg acgaaaccac
    39721 ggcacgagat ctgacgacac agatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc
    39781 tgtcagtggc ctgaacatcc ttaccctgat gaataatgat atggcgctga tacagctgca
    39841 ccatatatcc caacgggcga aatcccgctc cttacgtgat aacgcggcgg aatttcttca
    39901 ggtggtcgca gaaaatcgcg ggctaagcca ggaagagtta gcggacagat tagtcccaac
    39961 attgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc agtttaccgt
    40021 tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc gccagaaaag
    40081 tgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac tggcccgact
    40141 aaaagggcta aaaaaagatg ccactcaggt gagcaaaaac ctgctcccac gtcttgaagc
    40201 agccctacgt atcacccgcc gctggtcgct ggcagatttt cattctctgt ttgttaatca
    40261 tccctttacc cgcctggtta cccagcgatt aatatggggg gtttatccgg caaatgaacc
    40321 gcgtcgttta ctcaacgcct ttcgtgtggc cgcagagggg gagttctgca atgcacaaga
    40381 tgagcccatt gacctgcctg cggatgctct gattggcatt gcccacccgt tagaaatggc
    40441 agcagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta tgccgccttt
    40501 tcgccagttg gcgcggcgca cggtgctgct cacacctgac gagtcagcca gtaacagcct
    40561 gactcgctgg gaaggtaaat ccgctaccgt tgggcaactt atgggaatgc gatacaaagg
    40621 ctgggagtca ggctatgagg acgcatttgt ctatgactta ggtgcgtacc ggctggtcct
    40681 taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa tgagcttccg
    40741 ttctcttcgg gtttaccgtg acaataaatc cgtcactttt gccgaacttg atgtgtttga
    40801 tttgagtgag gcattaagcg cacctgacgt cattttccat taaaacacag cgggcagtga
    40861 tgcaactgcc cgttatcaac acgacctacc agcgggggaa gcgatgatcg tgcaaaaaga
    40921 gctggttgct atttacgatt atgagatccc tgtacctgaa gagccgtttt ccttcagact
    40981 tgaaatccat aaatgctctg aattatttac aggttccgtc tatcgactgg agcgattccg
    41041 gctacatcca acatttcatc aacgtgatcg agaagatgcc gaccactaat aaatgatgcg
    41101 ttgatttata taagagatga gtttattgat gagcggaaat tacgaggtga atcacatgaa
    41161 acagtaatag caatttttaa ttgtgaacta cagaatatat tcaaccaaga aatagaataa
    41221 tatactctag ataattcaag tcgcaggaag gcgataagcg agtgagtccc caggagctta
    41281 ctaaagtaag tgacaggggt gaacgaacgc agtcgcagca catgcaactt gaagtatggc
    41341 gagtatagct ttattaaagg gggaaacggt attccccctt agatgcgttt caatttgccc
    41401 gttacttcaa caaatcaatc aaccgtaaca gagccactaa tagctccagt acagcaatga
    41461 gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc ttttaaggag cgcaacacca
    41521 gcaccttacc cttacactgc ctgtcgatac tggtttacgt atgttggacc acgatgttgc
    41581 actccggatc aggcaccacc tgcagcacca cccgtccggt agccaggact tgaaattggt
    41641 tcgatccggc gcaacctccc aacagtcagg attataaatc tcattatctt aattgcaacg
    41701 gggtccagcg gtggttataa ttcgttcgtt ggaacacgat gttgcacagg ctgcggtgtt
    41761 aggcctgaaa gtaataaata aaagaacctg cctcaccagc aggttttttt attttttgcg
    41821 atctgctttc cagatatttt ccgctcaaac aactaatgcg ccaaacattt attgcgcgta
    41881 aaatatcgtt tatttcatta atacatttca gggatgaata tatgtcaccg cagaataatc
    41941 acctgcagcg tccgcctgct gctgtgttat acgccgttga actggcaaaa ttaaaacaaa
    42001 atgataacgc accttgcccg cccggttggc agttaagttt gcctgcggcc cgtgctttta
    42061 ttcttggcga cgaagcgcaa aatattcgcc gtaaagtggt gattagtccc tctgctgtcg
    42121 aacgcatgtt agtcactctt gccaccgggc gcggtttgat gttggtgggg gaaccgggta
    42181 ccgcaaaatc tctcctgtct gaattactgg caaccgctat tagcggcgat gccgggttaa
    42241 ccatccaggg cggcgcatct accaccgaag atcaaatcaa gtacggctgg aactacgccc
    42301 tgcttatcaa tcatggtccg tcaacggaag cgctggtccc tgccccgctt tacctgggaa
    42361 tgcgcgacgg caaaatcgtc cgttttgaag agataacgcg tactccactg gaagtacaag
    42421 actgtttatt aggtatgctt tctgacaggg ttatgacggt gccggaactc actggtgaag
    42481 ccagtcaact ctatgctcgg gaaggcttta atattattgc cactgccaat acgcgcgatc
    42541 gcggcgtcaa tgaaatgagt gccgcgctaa agcgtcgctt cgattttgaa actgtctttc
    42601 cgattatgga tttcgcccag gagctggagc ttgtcgccag tgcttcggcg cgtttgctgg
    42661 cccatagcgg cattccgcat aaagtgcctg acgcggtact ggagttactg gtccgcacct
    42721 ttcgcgatct gcgtgccaac ggcgaaaaga aaacgtcaat ggatacactg acagcgatta
    42781 tgtccaccgc tgaagccgtg aacgtggccc acgcggtggg cgtccgcgcc tggtttttag
    42841 cgaatcgagc aggtgagcca gccgatctgg tggactgtat tgccggaacc atcgtcaaag
    42901 ataacgaaga agaccgcgcg cgcctgcgcc gttacttcga acaacgtgtt gccacacata
    42961 aagaagctca ctggcaggct tattatcaag ctcgccaccg cctgccgtga ggaaagatgc
    43021 atgagcgagc cgttaattgt cggcatccgg catcatagcc cggcctgcgc ccggctggtg
    43081 aaatcgttaa tcgaaagcca gcggccacga tacgtattga ttgaaggccc ggctgatttt
    43141 aatgaccggg tagacgaact gtttttagcc caccagcttc cggtagctat ttacagttat
    43201 tgccagtatc aggacggtgc ggcccccggg cgtggtgcct ggacgccatt tgctgaattt
    43261 tcgccggagt ggcaggcgct acaagccgca cgtcgtattc aggcacaaac ttacttcatc
    43321 gatttgcctt gctgggcgca gagtgaagaa gaggacgatt cgcctgatac acaagatgaa
    43381 agccagacct tactgctgcg tgccacccgc atggataaca gcgataccct gtgggatcac
    43441 ttattcgaag atgaaagcca gcaaactgca ttaccctcta cgctggcgca ctattttgcc
    43501 caactgcggg gcgattcccc cggagatgct ctcaatcgtc agcgcgaagc ctttatggct
    43561 cgctggatta catgggcgat gcagcaaaac aatggcgacg tgttagtcgt ctgcggcggc
    43621 tggcacgctc ctgcactggc aaaaatgtgg cgcaaatgcc cgcaggaaat gaacaagcca
    43681 gaattgtcct cgctggcaga tggcgttaca ggttgttatc tcacacccta cagtgaaaag
    43741 cgccttgatg tgctggcagg atacctttcc ggaatgcctg ccccggtctg gcaaaactgg
    43801 tgctggcagt gtggcttaca gcaggcaggt gaacaactgc tgaaaacggt tcttacccgt
    43861 ttgcgccagc acaaattgcc cgcttctacc gctgatatgg ctgctgctca tctgcatgcg
    43921 atggcactgg cacagttgcg cggccataca ctacctctac gcactgactg gctggatgcc
    43981 atagctggct cgctgattaa agaagccctg aatgcgccgt tgccgtggag ctatcgcgga
    44041 gttattcatc ccgataccga tccgattttg ctaacggtga tagacacatt agcgggtgac
    44101 ggattcggta aactttcccc ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc
    44161 gaactggaac gtaccggaat ctctcttccg gcggagctta ccttaaatcg ctttaccccc
    44221 gatggactgg cgcaaagtca ggtgttacat cggttggcaa tactggagat cccaggggtt
    44281 gtgcgccagc agggaagtac gctgacactt gcaggcaacg gtgaagaatg ctggaaatta
    44341 acccgcccgc ttagccagca tgcggcattg attgaggccg cctgctttgg tgccacactc
    44401 caggaagccg cacgccataa attagaagcc gatatgctgg acgcgggtgg aatcggctgt
    44461 atcaccacat gtcttagcca ggcagcgtta gcgggtctgg cgtctttcag tcagcaatta
    44521 ctggaacaac tcacattatt aattgcccag gaaaatcaat tcgccgaaat gggccaggcg
    44581 ctggaagtgc tatatgcctt atggcggctg gatgaaatta gcggtatgca aggcgcgcag
    44641 atattacaaa cgacattatg cgcggccatc gatcgcacgc tgtggctatg tgaatccaac
    44701 ggcagaccgg atgaaaagga gtttcacgct cacctgcata gctggcaggc gctttgccat
    44761 atcctgcgcg atctacatag cggcgttaat ttacccggcg tttcgctttc tgcggcggta
    44821 gccttactgg agcgccgcag tcaggcgatt catgccccgg cgctggatcg cggcgcgact
    44881 cttggcgcac tgatgcgtct ggaacatccc aacgccagtg ccgaagcggc gctgacgatg
    44941 ctggcgcagt tatccccggc acaatccggc gaggcgctgc acggtttgct ggcattagcc
    45001 cgtcatcaac tggcctgtca gccggtattt atcgccggtt tcagcagtca tttaaatcaa
    45061 ctgagtgatg ccgattttat caatgccctg cccgatttac gcgcggcgat ggcctggcta
    45121 ccaccacgag aacgcgggac gctggcgcat caggtgcttg agcattacca gctcacacag
    45181 cttcccgttt cagcgctgca aatgctgttg cattgtccac cacaagccat tgcacatcat
    45241 caacaactcg aacagcaggc actggcatcg ctgcaacact ggggagtttt ccatgtctga
    45301 actgaacgat cttctgacca cccgtgagct acaacgctgg cgattaattc ttggcgaagc
    45361 ggcagaaacg accctttgtg ggctggatga caacgcccgg cagatagacc acgcgctgga
    45421 gtggctgtat gggcgcgatc ctgaacggct ccagcgtggt gaacgttacg gtggattagg
    45481 tcgttcaaat ctcaccaccc ctgagtggat taacagcatt cacacgctgt ttccgcaaca
    45541 ggtgattgag cggctggaaa gcgatgccgt gctgcgctac ggcattgaag atgtggtgac
    45601 gaatctcgac gtgctggaac gtatgcagcc ttctgaaagc ctgctacgcg ccgttttgca
    45661 caccaaacat ctgatgaatc ccgaagtact ggctgccgcc cgccggatag tgcaccaggt
    45721 tgtggaagaa attatggctc gactggcaaa ggaagttcgt caggcttttt ctggagtacg
    45781 cgatcgccgt cgccgctcat ttatttcact ggcgcgaaac tttgatttca aaagtactct
    45841 gcgcgccaac ctgcaacact ggcacccgca acacggcaag ttgtatatcg aatccccccg
    45901 ctttaacagc cgcataaaac gctatagcga acaatggcaa ctggtcctac tggttgatca
    45961 aagcggatcg atggttgatt cggtgatcca ctccgcagtg atggcggcct gtttatggca
    46021 gttacccggc attcgtaccc atctggtcgc ttttgacacc agcgtggttg atctcacggc
    46081 agacgttgcc gatccggtag agctattaat gaaagtgcaa ttaggcggcg ggaccaatat
    46141 cgccagtgct gtggaatatg gtcggcaact tattgaacaa ccagcgaaaa gcgtcattat
    46201 cctcgtgagc gatttttacg aaggggggtc atcatcattg ctgacgcatc aggtgaaaaa
    46261 gtgtgtccag agcggcatca aagtgctggg actggcggcg ctcgatagca gcgcaacacc
    46321 ttgctatgac cacgatacgg cccaggcgct ggttaatgtc ggcgcacaaa tagccgcaat
    46381 gacaccgggc gagctggctt cctggcttgc ggagaatctt cagtcatgaa ttcactacgt
    46441 ccggaattat tagaactgac gccgcaggct ctgacggcgt taagcaatgc cggttttgtt
    46501 aagcgcagcc ttaaggaact ggaaaatggc aacgtcccgg aaatcagtca tgagaacggc
    46561 gctttaatcg ccaccttcag tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg
    46621 aaagaggctc agtgcacctg cggggccagc ggcatgtgcc gtcatcgcgt gatgctggtg
    46681 ttaagttatc aacgactttg tgccaccgcc cagcctacgg aaaaaaaaga agaagagtgg
    46741 gatccagcaa tctggctgaa agaactggct aacctgcccg atgccacccg caaacgcgcg
    46801 caggcgctgg tcgctaaagg catcaccatt gagttgttct gtgcgcctgg cgaaattccc
    46861 tctgcccgat taccgatgag cgatgtgcgt ttttattccc gcagcagcat tcgcttcgca
    46921 cgctgtgatt gcattgaagg cacactttgc gaacatgtcg tactggctgt gcaggccttc
    46981 gtcgaggcca aaactcagca agcggaattt actcatttaa tctggcagat gcgcagtgaa
    47041 cacgtgacat catctgatga tccgtttgcc agcgaagaag gcaaaacgtg tcgtcaatat
    47101 gttcagcaat taagccaggc attatggctg ggcgggataa gtcaaccgcc aatccattac
    47161 gaggccgctt tcagtcgcgc gcagcaagcg gcggaacgct gcaactggcg atgggtgagt
    47221 gaatcgctac ggcaattgcg cgccagtgtt gatgccttcc acgcccgtgc cagccactac
    47281 catgccggag aatgcttacg tcagcttgcg gcattaaaca gtcgattaaa ttgcgtacaa
    47341 gagatggcgc gacgcgacag tatcggtgaa gttcctccca tgccgtggcg cacggtcgtt
    47401 ggcgctggca ttgccggaga agcaaaactt gatcatctgc ggctggtgtc tttaggtatg
    47461 cgttgctggc aggatattga gcagtatggt ttacgcatct ggtttaccga tcccgacaca
    47521 ggaagtattt tgcatctttc gcgtagttgg cagagaagcg aacaggaaaa ctcaccggca
    47581 gcgacgcgtc ggctgtttag ttttcaggct ggcgcactgg caggtgggca aattgtttca
    47641 caagctgcaa aacgcagtgc cgatggcgag ctgctgttag cgacccgtaa ccgcttgagt
    47701 agtgttgttc cactgtcgcc agacgcctgg caaatgttga gcgcgccgtt gcgccagccg
    47761 ggcattgtgg ctttgcggga atatttacgc cagcgtcccc ccgcctgcat acgaccactc
    47821 aaccaggtcg ataacttatt tattctgccg gtagcggagt gtatttcgct cggttgggat
    47881 agcagccgcc agacactgga tgcgcaggta atcagcggcg aaggagaaga taatgtgctg
    47941 acgttatccc ttccggcatc tgcctgttct ccttttgccg ttgaacgcat ggcggcgctt
    48001 ttgcaacaaa cagacgaccc cgtgagtctg gtttctggct ttgtcagttt tgtagagggg
    48061 caattgacac tggaaccacg ggtgatgatg acaaaaaccc gcgcctgggc gctggacgca
    48121 gaaactgcgc ctgtggcacc gctaccttct gccagcgttc tgcctgtgcc gtctaccgct
    48181 catcagttgc tgatgcgctg ccaggcgtta cttattcaac tgctccataa cggctggcgc
    48241 tatcaggaac agagtgctat tggtgaggca gagttgctgg cgaatgacct caccgcggtg
    48301 ggtttttatc ggctggcaca tgtgttggga caatttcgta atacagaaag cgaggcacgg
    48361 gtagaagcaa tgaataacgg tgttttgctt tgcgaacaat tattccccat gcttcagcaa
    48421 caaggatgaa atagtgcttt ttattaagag ttctactcct gttccggact gctcacgcca
    48481 cagtattagg catatcctat atagcccctg gtgagagtca ccagttcctt gattaaataa
    48541 aatggagttt tacatgaagg ctttcaataa gctgttttcc cttgttgttg catctgttct
    48601 ggttttctct cttgctggct gcggtgacaa agaagaatca aagaaattca gcgccaatct
    48661 gaacggcact gaaattgcca ttacctatgt ctacaaaggt gacaaggtgc ttaagcaatc
    48721 ttctgaaacc aaaattcaat ttgcctccat tggtgcaacc accaaagaag atgccgccag
    48781 gacacttgag ccgttaagcg ccaaatacaa aaacatcgcg ggtgttgaag aaaaattaac
    48841 ctataccgat acctacgcgc aggaaaacgt gactattgat atggaaaaag tggattttaa
    48901 agccctgcag ggtatttcag gaatcaatgt ttctgctgaa gatgccaaaa aaggtatcac
    48961 tatggcgcaa atggaactgg tgatgaaagc cgctggtttt aaagaag
//
LOCUS       DAOFSV010000019        48695 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.019, whole genome shotgun sequence.
ACCESSION   DAOFSV010000019 DAOFSV010000000
VERSION     DAOFSV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.019"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            207..632
                     /gene="nudI"
                     /locus_tag="QBH27_001199"
     CDS             207..632
                     /gene="nudI"
                     /locus_tag="QBH27_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDQ7013535.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(671..1273)
                     /locus_tag="QBH27_001200"
     CDS             complement(671..1273)
                     /locus_tag="QBH27_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDQ7013536.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1581..2720
                     /gene="arnB"
                     /locus_tag="QBH27_001201"
     CDS             1581..2720
                     /gene="arnB"
                     /locus_tag="QBH27_001201"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDQ7013537.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALATLPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2724..3692
                     /gene="arnC"
                     /locus_tag="QBH27_001202"
     CDS             2724..3692
                     /gene="arnC"
                     /locus_tag="QBH27_001202"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDQ7013538.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHVLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3692..5674
                     /gene="arnA"
                     /locus_tag="QBH27_001203"
     CDS             3692..5674
                     /gene="arnA"
                     /locus_tag="QBH27_001203"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDQ7013539.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKALPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5671..6561
                     /locus_tag="QBH27_001204"
     CDS             5671..6561
                     /locus_tag="QBH27_001204"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDQ7013540.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            6561..8213
                     /gene="arnT"
                     /locus_tag="QBH27_001205"
     CDS             6561..8213
                     /gene="arnT"
                     /locus_tag="QBH27_001205"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDQ7013541.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALMTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQGRLVLIQYRPK"
     gene            8210..8545
                     /gene="arnE"
                     /locus_tag="QBH27_001206"
     CDS             8210..8545
                     /gene="arnE"
                     /locus_tag="QBH27_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDQ7013542.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            8545..8931
                     /gene="arnF"
                     /locus_tag="QBH27_001207"
     CDS             8545..8931
                     /gene="arnF"
                     /locus_tag="QBH27_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDQ7013543.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8925..9191)
                     /gene="pmrD"
                     /locus_tag="QBH27_001208"
     CDS             complement(8925..9191)
                     /gene="pmrD"
                     /locus_tag="QBH27_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDQ7013544.1"
                     /translation="MEWLVKKSCCNKQNNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(9301..10656)
                     /gene="menE"
                     /locus_tag="QBH27_001209"
     CDS             complement(9301..10656)
                     /gene="menE"
                     /locus_tag="QBH27_001209"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDQ7013545.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AAQGVVAGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALTSLHIQQVEGVHAVAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(10653..11615)
                     /gene="menC"
                     /locus_tag="QBH27_001210"
     CDS             complement(10653..11615)
                     /gene="menC"
                     /locus_tag="QBH27_001210"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDQ7013546.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLMQLAR
                     IAAWLTPDTIPGLDTLDLMQVQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(11615..12472)
                     /gene="menB"
                     /locus_tag="QBH27_001211"
     CDS             complement(11615..12472)
                     /gene="menB"
                     /locus_tag="QBH27_001211"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDQ7013547.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12487..13245)
                     /gene="menH"
                     /locus_tag="QBH27_001212"
     CDS             complement(12487..13245)
                     /gene="menH"
                     /locus_tag="QBH27_001212"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDQ7013548.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13242..14912)
                     /gene="menD"
                     /locus_tag="QBH27_001213"
     CDS             complement(13242..14912)
                     /gene="menD"
                     /locus_tag="QBH27_001213"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDQ7013549.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERGRFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(15001..16296)
                     /gene="menF"
                     /locus_tag="QBH27_001214"
     CDS             complement(15001..16296)
                     /gene="menF"
                     /locus_tag="QBH27_001214"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDQ7013550.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGYLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTLLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTRTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16375..16680)
                     /gene="elaB"
                     /locus_tag="QBH27_001215"
     CDS             complement(16375..16680)
                     /gene="elaB"
                     /locus_tag="QBH27_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDQ7013551.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16735..17196)
                     /locus_tag="QBH27_001216"
     CDS             complement(16735..17196)
                     /locus_tag="QBH27_001216"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ7013552.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            17261..18178
                     /gene="rbn"
                     /locus_tag="QBH27_001217"
     CDS             17261..18178
                     /gene="rbn"
                     /locus_tag="QBH27_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDQ7013553.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEISTGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(18299..20041)
                     /locus_tag="QBH27_001218"
     CDS             complement(18299..20041)
                     /locus_tag="QBH27_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ7013554.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAA
                     QQAATKVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQI
                     ANPATARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEE
                     IVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKH
                     ITLLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPL
                     ASAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFI
                     RLIGLAKDLDNSQN"
     gene            20179..21150
                     /locus_tag="QBH27_001219"
     CDS             20179..21150
                     /locus_tag="QBH27_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDQ7013555.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDIPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQAPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQYD
                     IPAVMITDTAFYRNKQYHFPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            21253..21756
                     /locus_tag="QBH27_001220"
     CDS             21253..21756
                     /locus_tag="QBH27_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDQ7013556.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(21823..23280)
                     /gene="nuoN"
                     /locus_tag="QBH27_001221"
     CDS             complement(21823..23280)
                     /gene="nuoN"
                     /locus_tag="QBH27_001221"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDQ7013557.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(23287..24816)
                     /gene="nuoM"
                     /locus_tag="QBH27_001222"
     CDS             complement(23287..24816)
                     /gene="nuoM"
                     /locus_tag="QBH27_001222"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDQ7013558.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAIQALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(24980..26821)
                     /gene="nuoL"
                     /locus_tag="QBH27_001223"
     CDS             complement(24980..26821)
                     /gene="nuoL"
                     /locus_tag="QBH27_001223"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDQ7013559.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(26818..27120)
                     /gene="nuoK"
                     /locus_tag="QBH27_001224"
     CDS             complement(26818..27120)
                     /gene="nuoK"
                     /locus_tag="QBH27_001224"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDQ7013560.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(27117..27671)
                     /gene="nuoJ"
                     /locus_tag="QBH27_001225"
     CDS             complement(27117..27671)
                     /gene="nuoJ"
                     /locus_tag="QBH27_001225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDQ7013561.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(27683..28225)
                     /gene="nuoI"
                     /locus_tag="QBH27_001226"
     CDS             complement(27683..28225)
                     /gene="nuoI"
                     /locus_tag="QBH27_001226"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDQ7013562.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(28240..29217)
                     /gene="nuoH"
                     /locus_tag="QBH27_001227"
     CDS             complement(28240..29217)
                     /gene="nuoH"
                     /locus_tag="QBH27_001227"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDQ7013563.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(29214..31940)
                     /gene="nuoG"
                     /locus_tag="QBH27_001228"
     CDS             complement(29214..31940)
                     /gene="nuoG"
                     /locus_tag="QBH27_001228"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDQ7013564.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(31993..33330)
                     /gene="nuoF"
                     /locus_tag="QBH27_001229"
     CDS             complement(31993..33330)
                     /gene="nuoF"
                     /locus_tag="QBH27_001229"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDQ7013565.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(33327..33827)
                     /gene="nuoE"
                     /locus_tag="QBH27_001230"
     CDS             complement(33327..33827)
                     /gene="nuoE"
                     /locus_tag="QBH27_001230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDQ7013566.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(33830..35632)
                     /gene="nuoC"
                     /locus_tag="QBH27_001231"
     CDS             complement(33830..35632)
                     /gene="nuoC"
                     /locus_tag="QBH27_001231"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDQ7013567.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(35726..36388)
                     /gene="nuoB"
                     /locus_tag="QBH27_001232"
     CDS             complement(35726..36388)
                     /gene="nuoB"
                     /locus_tag="QBH27_001232"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDQ7013568.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(36404..36847)
                     /gene="nuoA"
                     /locus_tag="QBH27_001233"
     CDS             complement(36404..36847)
                     /gene="nuoA"
                     /locus_tag="QBH27_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDQ7013569.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETDSIANRQR"
     gene            complement(37477..38415)
                     /gene="lrhA"
                     /locus_tag="QBH27_001234"
     CDS             complement(37477..38415)
                     /gene="lrhA"
                     /locus_tag="QBH27_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDQ7013570.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQTMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            39335..40552
                     /gene="alaA"
                     /locus_tag="QBH27_001235"
     CDS             39335..40552
                     /gene="alaA"
                     /locus_tag="QBH27_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDQ7013571.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            40636..41235
                     /gene="yfbR"
                     /locus_tag="QBH27_001236"
     CDS             40636..41235
                     /gene="yfbR"
                     /locus_tag="QBH27_001236"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDQ7013572.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(41294..43126)
                     /locus_tag="QBH27_001237"
     CDS             complement(41294..43126)
                     /locus_tag="QBH27_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDQ7013573.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(43213..43863)
                     /gene="hxpA"
                     /locus_tag="QBH27_001238"
     CDS             complement(43213..43863)
                     /gene="hxpA"
                     /locus_tag="QBH27_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDQ7013574.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(43874..44368)
                     /locus_tag="QBH27_001239"
     CDS             complement(43874..44368)
                     /locus_tag="QBH27_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDQ7013575.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(44451..44906)
                     /locus_tag="QBH27_001240"
     CDS             complement(44451..44906)
                     /locus_tag="QBH27_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDQ7013576.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            45244..46446
                     /gene="ackA"
                     /locus_tag="QBH27_001241"
     CDS             45244..46446
                     /gene="ackA"
                     /locus_tag="QBH27_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDQ7013577.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            46521..48665
                     /gene="pta"
                     /locus_tag="QBH27_001242"
     CDS             46521..48665
                     /gene="pta"
                     /locus_tag="QBH27_001242"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDQ7013578.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
BASE COUNT        12071 a        13140 c        12159 g        11325 t
ORIGIN      
        1 caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt aaataattaa
       61 acgccgaaaa aataaacaca ctttatcgat acacacaaaa ataataataa atgtagttat
      121 catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat tctcatggtt
      181 tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc cctttgattc
      241 aaaatgatgg tgcttatttg ctgtgtaaaa tggccgacga tcgcggcgtt ttccccggtc
      301 aatgggcgct ttcgggtggc ggcgtggagc ctggcgaacg aattgaagag gcactacgcc
      361 gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg ccgtggacct
      421 tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag atttatatga
      481 tttacctgat ttttgactgc gtttctgcca accgagatgt gaaaataaac gaagagtttc
      541 aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttgaat gttgccaccc
      601 gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca ctggctgtca
      661 gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct ttttccacat
      721 gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta tctttagcaa
      781 tatatgtcag gcaatgatta tgggtaaaaa taacgatatt tttatcaggt gctttgcgtt
      841 gtaagtcttt aattgcacta taaatctcat taccgcattg aagaaatcgt ttatctaccg
      901 tcagcttttt acccgccgaa aaccaggtcg ccgactgaat agtccggacg gtattactgg
      961 aatagagatc aaaatcaggg atatcagcac taaaagcgtt gcccagttca cgggcatcct
     1021 gggtaccttt gaccgtaata cctgttttat ctgacaagca ttgattggtt gaacgatcgc
     1081 aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt ctggccagtg
     1141 ttttattgtc gatgcgtggc aaaccattgc tactccaggc ggcatgagtc cccagtccgg
     1201 caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaac gaagatcggc
     1261 aaaaagctaa cacgtaaact ccacctgtag acaagcgcaa ccagacaatt accgtgaaat
     1321 tgagcgactt ttctggcgat aattagcagt tggtgtaata ttaaaaatcc tatgatgccg
     1381 gcaaaatgcc tcaaaatttt gccaaatgca aagtctaaat aagaaaaaat ataaaaattt
     1441 caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc cttaaaattt
     1501 tgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt
     1561 caatgacgga tggaaaaaca atgtcaggat ttttgccttt ttcgcgacca gcaatgggcg
     1621 tagaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga
     1681 agaatcaggc gcttgaacaa gctttttgcc agttgacggg aaatcagcat gccatcgcgg
     1741 tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg
     1801 atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgctgg
     1861 gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga
     1921 tcgaagcagc cattacacca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc
     1981 cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag
     2041 atgctgccca tgccgtaggt acgtattaca aagggcgaca tattggcgca aaaggtaccg
     2101 ctattttttc gtttcacgcc atcaaaaata ttacctgtgc ggaaggtggc ctgattgtta
     2161 ctgataatga aaatcttgcc cgccagctgc ggatgctgaa atttcacggt ctgggtgtcg
     2221 atgcctatga cagacatacc tggggccgtg caccgcaggc tgaagtctta acgccgggct
     2281 ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta gtcaaattag
     2341 agcacctcaa cacccggcgg cgcgaaattg cccagcaata tcagcaagca ctggcaactc
     2401 tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta
     2461 ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa
     2521 aagaaagagg cattggtacc gggttacatt tccgcgctgc tcacacacaa aaatattatc
     2581 gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt
     2641 cattgccgct gttcccggat atgaccaccg ccgatgccga ccgcgtcatc acagcccttc
     2701 agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg
     2761 ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc accaccgcag
     2821 cctgtgaatc actggggaaa gagtatgaaa tcctgctgat tgatgacggc agtagcgata
     2881 attccgcgca tgtgctggtc gaagcctcac aagcggaggg cagccatatt gtgtctattt
     2941 tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt cacgtcaccg
     3001 gcgacttaat tattaccctc gatgccgatc tccagaatcc gccagaggag atcccccgtc
     3061 tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg
     3121 acagctggtt tcgtaaaacc gcttcgaaga tgattaaccg gcttattcag cgcaccaccg
     3181 gcaaagcgat gggtgactat ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg
     3241 cgatgttgca ctgccatgaa cgcagcacct ttatcccgat tctggcgaat atcttcgccc
     3301 gccgtgccat tgaaattcca gtgcaccatg ccgagcgtga gtttggtgaa tccaaataca
     3361 gctttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc
     3421 tacgtatgtt gagtttgctc ggcagcatta ttgcgattgg cggttttagc attgcggtgc
     3481 tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc
     3541 tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga ttgctcggtg
     3601 aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag
     3661 ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga
     3721 tatgggatgc ctcggtattg aagccctgct gtctgccggt tacgaaatta gcgccatttt
     3781 tacccatact gataaccccg gtgaaaaagc cttttatggt tcggttgccc gactggcagc
     3841 ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt gggtggaacg
     3901 cattgcccaa ctatcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga
     3961 tgagattttg cagctcgctc cgagaggtgc atttaatctg catggttcgc tgttaccaaa
     4021 atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaactggcgt
     4081 tacattgcac cgaatggtga aacgtgccga tgccggggcc attgtggcac aactgcgcgt
     4141 tgccattgcg ccagacgata tcgccattac gctgcatcat aaattgtgcc atgccgcgcg
     4201 ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca
     4261 gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt tcctcgaatg
     4321 gcataagccg gcatccgtac tgcacaacat ggtgcgtgcc gttgccgatc cgtggccggg
     4381 tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca
     4441 tgccagcaaa gcactgccgg ggagcgtgat ttctgttgca ccactgctga ttgcctgtgg
     4501 cgatggcgcg ctggaaatcg tcactggaca ggcgggcgac ggcattacta tgcagggctc
     4561 gcaattagcg cagacgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg
     4621 tgccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca
     4681 tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag
     4741 cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag gcgatatcag
     4801 tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtgt tgccgctggt
     4861 ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt
     4921 cgaagagaat ctgcgcatta tccgctactg cgtgaagtac cgtaagcgaa tcatcttccc
     4981 gtcgacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg atcattctaa
     5041 tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcta aacaattact
     5101 tgatcgggtg atctgggcct atggcgaaaa agaaggttta cagttcaccc tcttccgccc
     5161 gtttaactgg atggggccac gactggataa ccttaatgca gcgcgaattg gcagctcccg
     5221 cgctattacg caactcattc tcaatctggt agaaggttca ccgattaagc tgattgatgg
     5281 cggaaaacaa aaacgctgct ttactgatat tcgggatggt atcgaggcgt tataccgcat
     5341 tatcgaaaat gcgggaaatc gctgcgatgg cgaaattatc aacattggca atcctgagaa
     5401 cgaagcgagc attgaagaac tgggtgagat gctgctggcg agcttcgaaa aacatccgct
     5461 gcgccattac ttcccaccgt ttgcgggctt tcgtgttgtc gaaagtagca gctactatgg
     5521 caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc gccgctgcct
     5581 ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct
     5641 gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg cattgatgtc
     5701 gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat
     5761 aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg
     5821 cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc atcgctttat
     5881 ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat
     5941 gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac
     6001 catgcctggc aagcccatag cggtaattgg gatcggcaaa caatgatcga cgatattgca
     6061 cgcggccttc gcaccctgga agagattatc ggtcaaccgg taacctgttc tgccgctgct
     6121 ggctggcgtg ctgaccaaca ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac
     6181 agcgattgtc gtggggcgat gccgtttcgt ccattgctcg aatcaggaac ccccggcact
     6241 gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca
     6301 gaagatttta acggttggtt actcaaccgc atccagcgag ataaaggcac gccggtttat
     6361 accattcatg ccgaagttga aggctgcgct tatcagcata attttgtgga tctcctgaaa
     6421 cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc agggacgttg
     6481 ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa
     6541 caaatggcgg gtagtcactg atgaaatcgg tacgttacct tatcggcctc ttcgcattta
     6601 ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaacca gatgaaacgc
     6661 gttatgcgga aatcagtcgg gaaatgctgg catccggcga ctggattgtt ccccatctgt
     6721 tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat
     6781 ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa
     6841 ctgccgcgct gatgacctgg tttactctgc gcttatggcg cgataaacgt ctggctttac
     6901 tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc
     6961 tcgatccgtt tattgccttc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc
     7021 aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca
     7081 tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat
     7141 gggtagcaac gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca
     7201 gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag cctgacttct
     7261 ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata
     7321 gagcgccgtt ctggtactac ttgccggtca tcattgccgg tagcctgccg tggctgggat
     7381 tactccccgg tgcactgtac gctggctgga aaaaccgcaa gcattccgca accgtttatt
     7441 tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc aaattgccca
     7501 cctatattct ttcctgcttt gcacctctgg caatgctgat ggcgcattac gctttgctgg
     7561 cagcaaaaaa taatcctctg gcgttgcgga ttaatggctg gattaacatc gcttttggcg
     7621 tcactggcat tattgccaca tttgtggtct ccccctgggg accaatgaac acaccggtgt
     7681 ggcaaacctt cgagagctat aaagtctttt gtgcctggtc tattttttcg ctatgggcat
     7741 ttttcggctg gtacacctta acaaacgtcg aaaagacctg gccttttgcc gcgctttgcc
     7801 cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg gaaggaaaac
     7861 atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta
     7921 ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca
     7981 tgtatcgcca gacaggtgag ttgaaatatg gccttaatta tccggatgca aaagggagat
     8041 ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta
     8101 cactcgtgct ttcggttgac cgcgatgaag atatcaatag tctcgccatt ccgcccgctg
     8161 atgccatcga tcgtcaggga cgtctggtac tcattcagta tcggcccaaa tgatctggct
     8221 aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac
     8281 ctgctttgcg gcggtaaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct
     8341 ggcttgtctt ggccttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg
     8401 cattgcctac ccgatgttaa gtctgaattt tgtctgggta acgctggctg cagtaaaact
     8461 gtggcacgag ccggtatcgc tgcgtcactg gtgtggggtg gcgttcatta ttggcggcat
     8521 tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag cgtcattatc
     8581 gcctccgctg cgcaattaag cttgggtttt gcggcgagtc atctgccgcc aatgacgcac
     8641 ttatgggatt ttatcgcggc gctgttagct tttggcctgg atgcccggat attgttactc
     8701 gggctgctgg gttatctgct ttccgttttt tgttggtata aaacactaca taaacttgcg
     8761 ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg
     8821 gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt
     8881 atgagcgggt tgatgctgat tttcctgccc acaacaaaac aacgttactg agttttccct
     8941 gccgctttac actgccgttc ccactcatcg gggctataac agcttgcaga aagaactttc
     9001 accgtgtgca gcttttcacg attaatacag taaagcgcat tctgcaaagg cgagagtaaa
     9061 tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca
     9121 gcatcgcaga gcataagtac atgtctgtta ttttgtttat tgcagcacga ttttttgacc
     9181 agccattcca ttgcattatc ctgtttgata agagttttca catcaattta cagattaaca
     9241 gataaaccta ttatcaacgg caatggaatg gcaaaattgc gaagtgaaac taatgatttc
     9301 ttaatcctgg cgttgcaccc actcttttaa cgcctgacgt gaaattttaa taccgccgtt
     9361 tttcagctcc ggcggtaaag tcagccagcg caccggttgt tgaaaacggg ccagcttatc
     9421 tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg ctaccggtcg
     9481 atgaccaaac tccttgtcag caacgggaac gataaacacc tgcaaaaccg caggatgtgc
     9541 agcaataacg cgctcgactt cttctggctg aatgccttcg ccaccactga agaacagatt
     9601 gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtggc
     9661 gtaccagcct tcgtcattaa ccagtggaac ccgttgcccg ttacgccagt aaccttctgc
     9721 catgctggca gcccgcagcc acacttcgtc gtttacgatt ttcacttccc gacccggcag
     9781 cggcgaaccg acgtctgcca gaccgtcggc ttctttcgca cacaccgtgg aggcaaactc
     9841 ggtcagacca tagccgcaaa agcaccgaat cccctgctcg cgcgcctgtt ccgtcaactc
     9901 gaccgggata gccgcgccgc caagtaaaac cgctttcagg gaaacggaac tacggttaac
     9961 cagcaaacgc cagagttgtg ttggcaccag tgaagcgtgc gtacagcctg ccagcatttg
    10021 ctccaatggc tgtttatcac gtaccgtcat ccgcgcacca gcgtataacc agcgccacat
    10081 aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc
    10141 aaacggaatc agcgataaca ccccttcggc gctggcaaga tgagcctgat aagtatgaac
    10201 agcggctttc ggcaagccag tggaaccaga ggttaacgtc attgagcaca gacgcgtcgg
    10261 ctgccacgcc acggcatgta cgccttcaac ctgctgaata tgcagcgacg ttaacgccgg
    10321 aaacgtgttt tccccttccg gcaccagcgc aaattgcagc gtaagattgg gcagcaattc
    10381 ctcaagcagc ggttgcggca gctgagggtt cacgggcaac acccgcgccc cgcattgcag
    10441 taacgccagc caggccagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct
    10501 gcccgcaacc acaccctgcg ccgcaaagct ggaggctaat tcatcgacgc gagcacaaag
    10561 ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg
    10621 ccgccagtga cgccacggcc agtcagagaa gatcataaca gccgctccag tgcatcaacg
    10681 tccactaacg gcagtggact ccccggccag cgacgaacct gctgcacctg catcagatcc
    10741 agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg cgccagttgc
    10801 attagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc cagcgcatgc
    10861 gccgccttca cctgctcgcg tactttctcc aggctgcccg tgagcgtggg tttgataact
    10921 accgcgcgca cgccctcttc agctacaaag gcaaaatccg gctcgcgcaa gctttcatcc
    10981 caggcaatgg caatgccggt ttcacgggca aacgcacgcg aatcatcgcg ggttttgcac
    11041 ggctcttcga gaaatgcgat gcggtggcga taatccgggt taacgtattt ggcaaactgc
    11101 tggcctttca gcggtgtcca ggcgcgattc gcgtcaagac gcaaatgcag atccggaatt
    11161 gcctccagca acagattcac caccatgccg tcgcgcacgg cttcatacaa tcccactttg
    11221 accttcgcca ctttctcgcc aggcatatcg gcaagtttga ggatcagatc gtcaggatcg
    11281 ccattacaca gcggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc
    11341 gccaatgcac aacttacgcc aaaggcaacg gaaggcatct gcggtaattc gcaatcgcct
    11401 gccagccagt tatttaccca ggcaagcagc acactttgcg catcttccca ggtttcctga
    11461 ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcaccctc acgcaggcaa
    11521 acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg
    11581 gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga
    11641 aatcaggctg acgtttctgg ttgaaagcat tgcgaccttc ctgaccttct tccgtcatgt
    11701 agaacagcat ggtagcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg
    11761 cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcacggc
    11821 accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttt acaaggccca
    11881 tatccagcgc ctgtttcgcg tcgtactgac ggcacaagaa ccagatttca cgcgcttttt
    11941 tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt
    12001 tcgggccagt ctggccgaag atggcattat cagccgcgat agtcaggtcg cacatcatgt
    12061 gcagaacgtg accgccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac
    12121 aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg gaatcatctt
    12181 tatagccgcc gtaatcgcca cgcactttct ggtcaccacc ggagcagaac gctttatcgc
    12241 ctgcaccagt cagaatgatc acgccgatgt tgtcgtcata acgcgcatcc gccagcgcct
    12301 ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg
    12361 tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa ccttcggagc
    12421 agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc
    12481 ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg
    12541 gtgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag
    12601 agcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct
    12661 aaggttagca cgtaaatcgg gctggacggc gagagaagtc gcctccagca tggcggcaag
    12721 ggttgcgcca ttattgttgc tgcgcagcgc caccagctcc cggcgttgat cgtcattgag
    12781 cgaggcaaaa acaggctgtt gataccaatc ggcaaatacc gccgttaacg gttctgtacg
    12841 aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt cagcattttg
    12901 caacccagga tgcccgcctt cgacaacaac tccacaaagc cccgccagct cctggcaagc
    12961 cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt taaggatgtt
    13021 gtaactaacc agcgttttac acagtaagtc ggtgacatca tcaaatccat cgacgctgat
    13081 cgtcgctgaa ccaccgtgac ctgggagatc aacatacaac cgcgagtagt cggcaaacgc
    13141 ctcgcccact tcttgccatt catggcaatc gccggaaaaa ccgtgcaaaa acaccagcca
    13201 gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc
    13261 gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc
    13321 gtggtggtcg gcgtacgcca ggcatcgact agtgtcgttt caagttcctg ccagttttgc
    13381 ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc
    13441 atcagataga aacgcccgcg ctcgcttttc ggcgttggca acagcgaaaa aatttgcccg
    13501 ccgttgttgt tcaccacaat taataccagc ggtgcggaaa cctggcgcaa taacgccagc
    13561 gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt
    13621 gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc accacggttg
    13681 ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg caccaccagg
    13741 ctgttaccga caaacaattg cccctgttca ggcaaatagt cgctgatgcg atgcgccagt
    13801 tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg ttccgccagg
    13861 cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg
    13921 gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg
    13981 tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattggag gagtcgtttc
    14041 cccgtcaggc tgcttcccaa ttgcaccact atttgtgcct gttgcagttc gctggtcgct
    14101 ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg cgacagcacg
    14161 tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt gccctcttcc
    14221 gcactcatgc gcccggcaac caccacaccg cgcttttgtc gccagaagaa ccagtcgcgc
    14281 tgtttttcac tttcccgacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag
    14341 tcgcccagcc gctgttgcca gctaatcccc gtgtcgtcca tttcgccata cagcggttca
    14401 gcaaacgggc agttgatatg gactccacca gcatgaagcg taccgagagc gtggtcgatg
    14461 gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcagtga aatactgtgc
    14521 gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc gcagtcaatc
    14581 agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa cccagcttca
    14641 atcagcgccg gatagagatt tgctaccgcc gtgccggagg tcacaatcac cgccaccggc
    14701 tgcttgctga ctttcgccag ccccagcgcc agatgcccca gtccacgctc atcgaaatgg
    14761 gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag
    14821 ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc
    14881 gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct
    14941 aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta
    15001 ctattccatt tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc
    15061 ttgctcgggg tcggaaccac ggacaatgcc tgcgccagca tatagccgca cgatattgcc
    15121 gctgattttt gctgagcgca gggaaacgca gaattcactt tgctgtaatg aaagatagcc
    15181 cgcagaaccg gcgtaccatt ctcgagtgaa cggttcgtga cgggcgataa actgtcgcgc
    15241 cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac
    15301 atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgta ctttacgtag
    15361 acgcagtacc tgcggcggta aaacatccag cgtccgggta tcggcctgca atcgttggca
    15421 gatatcttcc accaccaaca tgttctcgcg ctggttttta tcatccgcca tcagccactc
    15481 acctaactgc tgcgcctgct tatcatcagg atgatttgct accgttcccg ccagcgcttc
    15541 ggtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagaac caagaaaagc
    15601 attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattgagcc gacgactggc
    15661 agccatcatc gctgccgcat tgaccggact tgcgaaatgc aggtcagttg cccgagcaag
    15721 cacaactttg tcgagctcgc cttcggcgat ggttttcgtt gccagttcga ttaatagcgt
    15781 ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta atccaggcag
    15841 gggcttgata ctcaccagtg tggcgataaa ttcttttgcc ttgattgcat cgtgctgaag
    15901 ggagctttcg ctgaatagcg tcaaccgcag cgtggcttta ccgccacaac gtcgccattc
    15961 caggcgtggt aaaagtaaat agccctgcga cgggtcaaac gcattcaacc cccagatgcg
    16021 taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa
    16081 acgggtaatc gcacccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa
    16141 ttgcggatac acctgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg
    16201 gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg
    16261 cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc
    16321 tcacattata cggggtacga caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg
    16381 gcgtgccagc aacagtccta gaaccagccc aacggccgca cccacaccaa ttccttgcca
    16441 gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata
    16501 actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc
    16561 tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag
    16621 tgtttcacta agcagcgtca ggtcgtcatc aatacgtgta tcaccaaact gattagacat
    16681 tctcgttctc catttgtgta aaacctgtca ctaactatag acaagggtta ttgattacgc
    16741 ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat aaatatcggt
    16801 cacagggatg aaaccaaatt gcccataaaa gttttgtaaa tgcgcctgcg cccccagata
    16861 aatcggcttc tccggccagc ggcgagtaca cgattccagc gccttactca tcaattgctg
    16921 accgattttt tcaccgcgta aggcttcgct gacaatcacc cgaccaatca caacgggttg
    16981 cagttcgtcg tcacttttca gaatcctcgc atacgccacc agtgtcccgt tatgccaacc
    17041 gagaatgtgg cgattttctc cctccagatc gtcaccatcg atatcctggt aaggacagtt
    17101 ttgttccact acaaataccg cacaacgcag ttgtaataag gcataaagct gagaaacgga
    17161 taaatcagaa tgatgcagat cttgccagtc gatcatggaa ggctcctttg tgctgtttgc
    17221 tttatactag ccggaatatt ttcagcagcg tgatgaactc atggaattaa tttttttagg
    17281 tacttcagcc ggtgtgccaa cccgtacgcg taatgtcacg gcaatattgc ttaacctgca
    17341 acatccgacc cagagcggac tttggttatt tgactgcggt gaaggcaccc agcatcagct
    17401 actgcatact gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg
    17461 cgatcatctc tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca
    17521 acccttaacg atttatggtc cgcacggtat ccgtgaattt gtggagaccg cgctgcggat
    17581 tagcggctca tggaccgatt atccgctgga aattgtcgaa attagcactg gcgaaatttt
    17641 cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg aatgttatgg
    17701 ttatcgtatt gaagaacatg ataaaccagg tgcattaaat gcccaggcat taaaagctgc
    17761 tggcgtgccg cctggcccgc tgtttcagga attaaaagcg ggcaaaacaa tcatgctgga
    17821 tgatggaagg cagattaatg gcgcagatta cttagccgct ccagtgccgg gtaaagcgct
    17881 cgctattttc ggcgataccg gcccctgcga tgccgcgctg gagcttgcta aaggcgtcga
    17941 cgtaatggtt cacgaagcga ctctggatat ggcgatggag gccaaagcca acagtcgcgg
    18001 ccatagttct acacgccagg cggcggcact agcccgtgag gctggagtcg gtaagctaat
    18061 cattacccac gtcagctcac gctatgatga caaaggttgt cagcacctgt tacgtgaatg
    18121 tcggtcaatt ttcccggcga ctgaactggc gaatgatttc gcggtgttta gcatttaagt
    18181 cgaaagcggt ggttagttag cagattaatt taacgatacc aacacattta tttgacattt
    18241 tttcatctgt ttgccggata cgtttgatgt cgatatcagg caaacagaat tatcattttt
    18301 agttttgtga attgtccaga tctttcgcca gtccaatcag gcgaataaat tccgctctgt
    18361 aaccctgtgg atcttcccct tttgccttct gagcccactg tttgatctgc tgccaggagg
    18421 tattgttcag gtattcagaa ccgcgtaact tttgcccata tgcagccact gctgcgcgaa
    18481 agcgcatatc ttctgaaggt gcctttattg cagatgccaa cgggaattca actaactgac
    18541 tttcttttcc ctgtggggat ttccaacgaa tctttaacca ggcaagttct tttgttttgt
    18601 ccgatttcgc tgatttgtta tccggggcat agcgtaactt atcaattgat gctttttgcc
    18661 ctttcagcgt taattcaaat aacaatgtta tatgtttacc tgcgccaata tcacccgcat
    18721 caacgttgtc gttattaaaa tcctctgcct taagttggcg cttttcataa cctatctgac
    18781 ggtattccgt tacccacgcg ggattaaact caatttgcgc tttgacatct tttgctacgg
    18841 taatcaacgt ctgccgcatt tcactgttca acactttctg cgcctctgag agggtatcaa
    18901 tgtagctata gttgccgtta ccaacatcgg caattctcac catcattgcc tcgttataat
    18961 tgctatcccc cacgccaagc gtcgacagag taacaccgga ctcacgctgt tttttgacca
    19021 ttgattcaat cgattttgga tcgtcaatgc caacgttaaa gtcaccgtca gtggccaata
    19081 aaatgcgatt gataccacct ttaataaacc ccttcgctgc ctgctgataa gccatctcca
    19141 gcccggcacc gccattggta ctgccttctg catccagcga atcaattgca gcattaattt
    19201 ccgccttgtg actaccggag atagaaggca acgcaatacg agagtcgcca gcgtaggtca
    19261 cgatggcaat gttatcctgc tcacgaagtt ctttgaccaa taatttcaac gaagactgga
    19321 taagtggcaa acgttcatca gaaatcattg aaccagacgt gtcgataaga aagaccagat
    19381 tagaagcggg taactcttca cttttgcgat ctttcgccag gatatcaact ttcagcaatg
    19441 ttcgctgttc attccagggt gcaggtgcca attcgtagcg catagcgaaa ggtattggct
    19501 tagaggccgg aatagattgt ttgtctttga tatcccagtc agacgggaaa taattgacta
    19561 tctcctccac ccgcacagcg tctggcggag gtaacagccc ttgattaagg aaacgccgca
    19621 cattcgcata actgccggta tcgacgtcaa ggctaaacgt cgccaacggg ttttgcgcta
    19681 cctgcttaac cggattatca tcgaactgct ggtagcgagc ggttgctgga tttgcgattt
    19741 gggaggctgc tttttctctg gctgcatgtt gatattttgg agccgcctgc aatcgcccca
    19801 gcaaagcctg tttgtctgaa tattgttgca cttcttgctg ggctaaggct ttcgcgtctg
    19861 cagcggcttt ggcggcgctt tgctcagcca cttttgttgc cgcttgttgc gcggcaagca
    19921 cttgctggtc agtgggtgta gtagattgtt gttgctgact tttcttatcc tcaggttccg
    19981 gcccacatcc tgacaaaatc aaactactta taagcaacat gattatattt ttatttcgca
    20041 tataaaatcc ttttaaataa actcagggcc ccgacataaa atacactctt tgtataatcc
    20101 tgctaatctt aataaaatac aatatcgaaa cccataacaa ataatgattc aacaacgcaa
    20161 cccattaata atttgcatat gaaaaaaata aattttgctt ttattatatt atttctattt
    20221 tcactccctc tgattatttt ttaccaaccc tgggtgaatg cattaccacc aacgccccga
    20281 cacgctagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca
    20341 cgtagcgccg acaatattga taatctgaat agatccgcag agtatataaa agaggtcttt
    20401 atcagtagcg gtgccagagt taccgcgcag gatatcccca ttacgggtgg cccctacaaa
    20461 aacattgttg ctaattatgg tcctgccgat ggaccgctga ttattattgg tgcgcactat
    20521 gactctgtca gtagttacga aaacgatcaa ttgacctata cgccgggcgc ggatgataac
    20581 gccagcggtg tggcaggatt actcgaactg gcacgtttgt tacagcagca agcaccgaaa
    20641 acaggcgtgc agctggtcgc ctatgcgtcg gaagaaccgc ccttctttcg tagcgatgaa
    20701 atggggagcg cggtacatgc agcttcgctt gagcgtccag tgaaattaat gatagcgctg
    20761 gagatgattg gttattacga ctctacgcct ggtagccagg attacccatc cccggccatg
    20821 tcctggcttt atcccgatcg gggagacttt attgccgtgg tcggcagaat gcaggatatc
    20881 aacgccgttc gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct
    20941 atgaatgccc caggttttat tcccggtatt gatttctctg atcaccttaa ttattggcaa
    21001 tacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac
    21061 tttcccggtg atatcgcaga cagattaaat tatcagaaaa tggctcaggt agtggatgga
    21121 gttacaacct tgttatacaa cagtaaataa taaaaatttc tcgaggcgga tccagaaaaa
    21181 gcaacatgcg cattttattt tctttcgtct gttttaagat gcgaaacatg aaattcaacg
    21241 gaggaaaaat aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac
    21301 caactgcttg agtctactaa aaaacctttg atggataata tacatgacac gctttcagga
    21361 ttacgccgac tggatattga taaacgctgg gattttttac actttggcct gacgggtaca
    21421 tctgcatttg accccgcgaa gaatgacccg ctaagtcgcg cagtgcttgg tgaacacagc
    21481 ctagaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
    21541 atcgaccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga
    21601 ctcaatgaaa tggagattta tccgggagta accttttcag aagagttaga ggggcaactc
    21661 tttgcttcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga
    21721 caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagggcac ccaaaaaaac
    21781 cgccgaacat gtcggcggct ttctgactta caaactgact gcttacatca gcggcattgc
    21841 taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc
    21901 agagatcagc accacgatac cgcctgcgct gtactgccag tttgatggtg catcgcgacc
    21961 cggttgttcc ggagcgtgaa ggtataagct caccgccaca cgcaggtagt agtagaggcc
    22021 gattgccgaa ccgacaacta cggcacccac cagccaccac aagtgtgcct ggacaccgac
    22081 cgccagcacg tagaacttac cgataaagcc cagcgtcatt gggataccag ccagtgaaag
    22141 catcatcacc gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga
    22201 gaacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa
    22261 cgcgccgagg ctgctgaaca gataacctgc caggtaaacc cctaccgctt ccatcgacat
    22321 ctcgccggtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga
    22381 gtaaccgagc agacgtttga tattggtctg gctcagcgcc atcaggttac cgaagatgat
    22441 ggaggcaaag gcgataatcg ccagcactac gcgaatcgct tcactgtcac ccaccggtgc
    22501 gtaaaggaac aaacgcatca ccacgccgaa gatagcgatt ttgctcgccg tcgccaggaa
    22561 agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac
    22621 cagagagagt ttgaagccga ggccaacaat catcaggccg aaacctgcca gcaacagcgg
    22681 ctcgttgagc ataccgtcgc cgaggttttt acccagtgcg acaaacgaca ggtcgccaga
    22741 ctgcgcatac accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat
    22801 ggtgtatttg atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc
    22861 aaacagcggc aaagagatca gttcgatacc gaggaacaga gacgccagat ggttggcatt
    22921 cgccagcagg atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt
    22981 gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa
    23041 taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagtggcg taacgtccat
    23101 agcgcccgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc caataaccga
    23161 gagcgtagcg ttgagaaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac
    23221 cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat
    23281 ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat
    23341 cgcggagtgc gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac
    23401 caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc
    23461 aatctggctt ttcgctttac cgaaataagc gcgatgcagc atcgccagcg aataaacaga
    23521 tgcaaagacc agcccaaagg tagagataac ggtaattacc gggacaacct ggaagctacc
    23581 gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac
    23641 cgcaaagaac agcgacagtg ctggcagcca cttcatcttg ctccacagac cgcccatcat
    23701 gcgcatgtcg cgggtatgga tacgttcata gagctgaccg cagagaataa acagacccgc
    23761 cgctgacaag ccgtgtgcaa tcatctggat taccgcgccc tggtaggcca actggctacc
    23821 ggtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg
    23881 tttgatatcg gtctgggcga aggccatcca cgcaccatag aagatgccga taacacccag
    23941 ccacatagcg attggcgcga actctgccga cgcgttcggg aacagcggta gggagaaacg
    24001 cagcagaccg taagcggcgg ttttcagcaa gatccccgcg aggtcaacgg aaccggcggt
    24061 cggagcctgg gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttcac
    24121 tgcgaaggcg atgaagaagc ccagcatcaa caggtattcc acgccgttag acatcggcgt
    24181 attcagcagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacgaaaac
    24241 cagcgcctgg atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt
    24301 ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag
    24361 gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc
    24421 aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg
    24481 gtatttttcg atctctttcc acgaacagag taccgccagc acaccgagca gaccggtcag
    24541 cacgaccatc agcaacgaca gaccgtcaat ggcgagatgg atagagatac caaaacgcgg
    24601 gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga
    24661 ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggttaccag
    24721 cgcgatccag cgcggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc
    24781 gataaaggga attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag
    24841 tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg
    24901 gtagtgtcgg atgcgccgcg aagacggctt atccgaccta cgttcttata tgcccagggg
    24961 caatcccaca atccttaact caacgcagta ccatcaacag agccagcacc acgaccgcac
    25021 cgatgctcat ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt
    25081 tacctgcaaa gcgggaaagg acagccggga tgttcatcat tgagttcagc ggatcgcgtt
    25141 tcagcaacca ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa
    25201 agccccaggc gttgtaccac caggtaccca gcagacggcc cggcgcactg ttggcgatgg
    25261 aggtcaccag agtacgttta cccagccaca gccaggcagc cagcagaatg ccgaccaccg
    25321 cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagc tccgtcgttt
    25381 gcggaagcac gccctgcagc ggcggtacaa tcagtgcacc aacgaaggtg gaaaggatca
    25441 gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt
    25501 cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac
    25561 cgaccagacc tgccaccatc agattgatat gtccattcgc catcgcaccc gcgaggatct
    25621 catccttact gaagaagccc gcagtgacca gcggcagtgc cgatagtgct gcgccgccca
    25681 ccaggaagca gagataaacc agcggaatag atttacgcag accacccatc ttaaagatgt
    25741 tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa
    25801 agaacgcgtg ggtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga
    25861 ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct
    25921 gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat
    25981 gcagaacttc cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg
    26041 cggttaccat tgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt
    26101 cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca
    26161 tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca
    26221 ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagc gcaaaagcga
    26281 ggaagacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg
    26341 gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga
    26401 ggtacatcaa cagtaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt
    26461 tggtgtaagc gaagaagcga gagtagccct cttctccgcg catataccag gaggcataca
    26521 tgtgaataag gaaacccaca ccagtgacca ctgagagcat ggtcagcgac aggccgtcca
    26581 gcaccagatt gaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc
    26641 tatatgcctg ctcaccgtta gcgaagaagt caacgccgat aaaggcggtt accagcgccg
    26701 ccaggcccac agagccaacg ccgacgatcg ccgagacgtt ttcagaccag cgcccacggg
    26761 agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca
    26821 tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag
    26881 cagcagcgca aggccgatac tcgcttccgc cgccgcgagg ctgatggcga gaatgtacat
    26941 cacctgaccg tcggtctggc cccagtagct tccggcgacc acaaaggcca gcgcggaggc
    27001 gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc
    27061 ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat
    27121 gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc
    27181 gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa
    27241 gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg
    27301 attggcgtac cgtcgatacc ttgatcgtta acaccgagaa tggcgtaaac aatcaccacc
    27361 agcatgatgg ctgacaaaat tgccggacca atccacacct gcggtttcaa ccactggcgt
    27421 tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc
    27481 ataatggcac ccgcatagac gataatttcc agcgcaccgg caaagtaagc gcccagtgag
    27541 aagaacaccc cagagatcgc cagcagcgaa ataatcaggt acagcagcgc gtgtaccgga
    27601 ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa
    27661 gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt
    27721 cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt
    27781 tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat
    27841 cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac
    27901 aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg
    27961 gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg
    28021 ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccggatcg cgggtcagaa
    28081 cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt
    28141 cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac
    28201 ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat
    28261 gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa
    28321 ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat
    28381 cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca
    28441 gccaccgaag aacagcgtca ccatcagtgc agagatggtc acaatcccga tgtattcgcc
    28501 cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc
    28561 ctgctcggct tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc
    28621 aaaggtaata aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac
    28681 gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaagg aaagcccgag
    28741 gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt
    28801 gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag
    28861 gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat
    28921 tgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg
    28981 atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag
    29041 ctggagcgaa ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag
    29101 caggcgacgt tcgccaaagc tcatgaatgc cccacaggtg actaccacca gcaggatcac
    29161 caccgcttta aggacggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt
    29221 tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata
    29281 cccatcggta agcccacctg ccctgccgtc agcccttcgg cgatttcaac cggcagcgtg
    29341 accgtgttgc catcgtaact aaaggagacg cgtgtacctg cgttcacccc caacttcgcg
    29401 gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca
    29461 cgctgtgaca attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg
    29521 tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt
    29581 tcaaacagac gcacgcccgg atcgccaaaa cgcagtttgc cgcccacttc gtcctggaat
    29641 ttgttccacg cctgcgggga gttccagcct ggtgcccagg caaacggcac ttgcgaacgg
    29701 tgcgcggtcg gctggttgtt accttccatc gagaaggtga acatagtgtc aatatcctgc
    29761 ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctatagcga
    29821 tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct
    29881 ttgatacctg ccagttccgg gattttcgcc acaacagcgt caatcacgtg gtcgagctgc
    29941 gtccagtcca cttcacggct cagcagggtg ctatgcagcg agtgcagcca gcgccagctt
    30001 tccagcatga cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg
    30061 gcgcggcctt cgttgttgat caccgtaccg tcgctttcag caaagctggc ggcggaaagt
    30121 accagatggg cgttttccat aatcgctgtg cgttgatggt caaccaccat caccagcggc
    30181 gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag atcgttttcc
    30241 agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgat
    30301 ccaccgccca taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgataccg
    30361 acgtcagcgc cgcgaccttt cagggctttc gccacgttag ccgcagcctg aatcacttca
    30421 gcgctaccag cgttcgtccc ggagataatc aacggtttct tcgcaccagc cagtgcctgc
    30481 acgatgacgt cgattttgct ttgcagctca ggctcaatac cgtcaaccgc tggtgcagag
    30541 ttatccagcg catgagcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg
    30601 taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga
    30661 tgcttcgcac gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt
    30721 gctgccgcca tttcgcgcgc tttacctttc accgcctgac gcactgccag cgcgacgcgc
    30781 gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg
    30841 cgcagagccg gagtataaat gccgccttca cgcagtactt tcagcgccag ttgcagacgt
    30901 tcctgctcac cgtgagcgat accggtatag aagttttctt cgcccaccag ctcacgcagc
    30961 gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga
    31021 cgcagaatat ctgctgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg
    31081 ccacgacgct gtactggctg acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa
    31141 cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga
    31201 cgcagttcgc cataacgctc gccggggctg atgttacagc cgatggaaca ttgctggcag
    31261 atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg
    31321 gtgaatacgc cggtcggaca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg
    31381 ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc caggtccgta
    31441 ccgtcagcgt aatctttgta gtaacgcacg cagcggtagc aggcgatgca gcggttcatt
    31501 tcgtgagaga tgaacggccc caaatcctga ttacggtgag tacgtttggt gaaacggtag
    31561 cgacggaagc tgtgtccggt catcacggtc atatcctgaa ggtggcagtt accgccctct
    31621 tcacataccg gacagtcgtg cgggtggttg gtcatcaacc actcgaccac gctttcacgg
    31681 aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata
    31741 caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac
    31801 tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg
    31861 cccagagaca gacaagcttc cagcaggttg tccgccccgt tgacctcgta ttctttgccg
    31921 tctacatgaa ttgtagccat tagcatgctt ccagttttct cagtcagaga ctgagcgtta
    31981 atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg
    32041 ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc
    32101 gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac ccggacctaa
    32161 gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tgaccttcac cacgctccag
    32221 cgcacgcaga attttcacgc tccacggcag accgtcgcgg catggcgtac accagccgca
    32281 ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt taatctcgtg
    32341 gtcaaccgcc atcgccagcg ccgtacccag acggcttccc gctttaccga tactttcgaa
    32401 ttccatcggc aggtcaaggt gcgcttcggt caggaagtcg gtgcccgcgc cgcctggctg
    32461 ccaggctttg aatttcaggc cgtcacgcat accaccagcg taatcttcaa ggatttcacg
    32521 cgcggtggtg ccgaacggca gttcccacag acccggattt ttcacccgac cggagaagcc
    32581 catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt
    32641 agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttaacgcagg ttggtttacc
    32701 ccatgcaccg gaggttgccg ggaagggcgg cttcgagcgt gggttagcgc gacgcccttc
    32761 cagggagttg attaacgctg tttcttcacc gcagatgtag cgccctgccc cggtatggac
    32821 gaacagttcg aaatcgaaac ctgttcccat aatgtttttg ccaagcagac ccgcttcggt
    32881 ggcttcggca atggcacggc gcagattcac tgccgcttca atatattcgc cacgcaagaa
    32941 gatgtagcca cgatacgctt tcagcgcaaa cgcagaaatg agcatacctt ccaccagcag
    33001 gtgcggtagt tgctccatca gcagacggtc tttataggtg cccggctcca tttcatcggc
    33061 attacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag
    33121 gccagtagaa aagcccgcgc cgccgcgccc tttcagacca gagtctttta cctgattaac
    33181 gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcg ccttcgtaac cgtttttgct
    33241 gcgatattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg
    33301 cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct
    33361 tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag
    33421 ttccccaggc agcaagttgg cagcagcgta aagcgaccgt caaatgtcgt ttgccctggt
    33481 ttgatgttca gttttttctc cagcgccgcc tgaataccct ggtaaccgtt gatatgacag
    33541 accacgctgt cgcaataacg gatcacatgg cggcctactg gctgacggaa gatctggcta
    33601 taaaacgttg ccacgccttc gacgtcgctc gccggaatac ccagcacatc ggcgatcgcg
    33661 tggatcgcgc catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg
    33721 gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc
    33781 gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca
    33841 catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc
    33901 cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtacgg atacgggtgc
    33961 gataactcat ggtgctgccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct
    34021 caaccatctg gaaggattca ttggcaggca tcaccggacc ccacgacact tgcaggaagt
    34081 gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg
    34141 gatgatccgc cttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca
    34201 gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac
    34261 cacccaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca
    34321 cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct
    34381 ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggtg ttttgcagcg
    34441 ccgctttctc gtaagacgcc agacgtttag gcatccagtc gaggaactca cgcagcaggc
    34501 gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca
    34561 tacgaaaacc agtgattgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga
    34621 agaacactgg cgtcatcgcg ccgacgtcct gaataaaggt ggagatatac agcaggtgac
    34681 tgttgatgcg gaacagttcg gagagcatta cgcgaatgac gttaacgcga tccggcacgg
    34741 tgatcccagc cagtttctct accgccagca cataaggcat ttcgttaacg cagccgccga
    34801 ggtattcgat acggtcggta tacggaatgt agctgtgcca ggactggcgt tcgcccattt
    34861 tctcagcgcc acggtggtgg taaccgatgt ctggtacgca gtcgacaatc tcttcgccat
    34921 caagctgcaa aacgatacgg aaagcaccgt gcgcagacgg gtggttcgga ccgaggttga
    34981 ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg
    35041 tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg
    35101 tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga
    35161 tgcggcgcag gttcgggtga ccgtcgaatg taatgccaaa tagatcccag gtttcacgct
    35221 cataccagtt agcgttcggg aatagtttgg taaaggtcgg tacgtgcagg tcgttttctg
    35281 ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa
    35341 cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt
    35401 gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg cccacttcca
    35461 gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa
    35521 aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca agatgatcgc
    35581 gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg
    35641 gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa
    35701 aattcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg
    35761 caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct
    35821 gatcgccaac cacccaggag agcggacgac gttctttacc gatagattcc tgcaacagca
    35881 tcagtgcctg catgtacgct tcaggacgcg gcggacagcc cgggatatac acatcaaccg
    35941 ggatgaattt atcaacgccc tgcacaacgg aataaatatc gtacataccg ccggagttgg
    36001 cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac aaacgctgaa
    36061 taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg
    36121 gcgaagcacg caatacttca gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag
    36181 tcaccatctc aacgtaacag caggaaaggc cgaagttata cggccaaatt gagtttttac
    36241 gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct
    36301 ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg
    36361 gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag
    36421 cgatactgtc cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca
    36481 gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag
    36541 cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca
    36601 gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc
    36661 gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggaacgttt tttgacctcg
    36721 cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg
    36781 caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca
    36841 tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac
    36901 gccacatcga ttcatgggga ggaataaaaa aaaccttaca ataactgcag aaactatttt
    36961 atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa
    37021 ggtttcttca cactgattta cacaatgaca acaacatttg catttggcgc cctttctccc
    37081 cagatcattg acgactccag gaaaattaga tgttgttgaa tcgtgtccgt tgtgaagcaa
    37141 tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga
    37201 gtattttctt gatctgtgac acgcttttgt cattctataa caaaaacgca gcaacaaatt
    37261 cacgtatttt ttaacattat tgtagcagga gatttttttc aggtgattat ttgtgcgtac
    37321 ggggcgtgaa tctctggtga ttgaaaaatg aacagtttta tacgttctgc acgatacgag
    37381 gaaggctttt tactaaaaaa aagccgctgg ggtttaaaat aaacccagcg gttcgttttt
    37441 acactattgt ctcaggaatt atctatcgtc cgtcgattac tcgatatccc tttcaatcaa
    37501 caatgagtca tctccttccg gagcagacat tgggctgtat tgccacggat tatggtagct
    37561 ctccatcgtc tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag
    37621 caaatattcg gtatcaggta gcggcggtaa gccatctact ccactcagca cacgcaggtc
    37681 cggactcatc atctcgaccg gccttgccgt cacgccaaga ccggctttca ctgccgcacg
    37741 aacggccgga agcgtcgagg cgacataagc cagtcgccag ggaatatctg ctttattaag
    37801 cgtcgccagc accatatcgc gaaacggact tggatcatcc agcaacacaa gagggatcgg
    37861 ctcacctttt tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag
    37921 gttcaatgcc ttaaaggtag aaggacgatg ggtggttacc atcaaatcca cttcctggga
    37981 ttccagcatt tctgccatgt aggcattacg tttaacgcgg acatccagcg ctagtttcgg
    38041 ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga
    38101 agcaccgata gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc
    38161 attaaaacgc aggattttcc tggcgtaacc aagaagttga atgccatgtt cagttaacag
    38221 tttgttgcga ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat
    38281 ttgctgactt acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt
    38341 cagatcggca acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg
    38401 atttgcactt atcatatatt atcacttact ggcggctcat actgagctgg ttaatgctgt
    38461 gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc atccggaacc
    38521 cggacgaaag taaaaatgca tatgagttgc actaaaaaag cgactcacat tgttccgtta
    38581 taatgcctga agtagatcac agaatatatc ttcagggatc gcatatctat taagttactc
    38641 actcttttct atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc
    38701 actctcgtac agcatcgctg gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc
    38761 atcaagagca gggatccgtc atttatgcaa gcattttacc ccaaaaacct tttatttata
    38821 agggtcattg cgaattatct gatgcaaagt tatgttatgt taggcaaagt aatcttctat
    38881 tattaataag cacaacaaaa cctttttgaa tattaaataa taattaatta gcttcatccc
    38941 cattcattaa ttccgcttaa caatagtttc acaccttgcc cccattgtga cacctcggaa
    39001 atcatcacgc tgttaaactc tccgtaatgc ggaaatatca tcatcgcgat tcagtagtga
    39061 attcttaaac gaggcatttt cacacaatta tcttacagat aaaaaaacca cacttacaat
    39121 taagaatcag aacaattcac tacataacat tacttattaa gcatatgcac ctcattattt
    39181 tgccattatt aagttattaa cagcacaatc gagccttccc ctctggcaaa atcttattct
    39241 gcagaccttc aaaacaccgt cctgggggag tacattgttc taagctgact tccacggcag
    39301 ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa
    39361 attagagaat gtctgttatg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga
    39421 agaagaaggt aacaaggttc tgaaactgaa catcggcaac cctgccccgt tcggttttga
    39481 cgcgccagat gaaattctcg ttgacgtgat acgcaacctg cctaccgctc aagggtattg
    39541 cgattccaaa ggtctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg
    39601 catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg agcttatcgt
    39661 tcaggcaatg caagcattgc tgaacagcgg ggacgaaatg ttggttcctg cacctgatta
    39721 cccactatgg accgcggcgg tttcactctc cagcggtaaa gcggtgcatt atctttgcga
    39781 tgaatcctct gactggttcc cggacctcga tgatattcgc gctaaaatta cgcctcgtac
    39841 gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct
    39901 tttaatggag attgtggaga ttgcacgtca gcataatctc attatctttg ccgatgaaat
    39961 ttatgacaaa attctctacg acgacgctga gcatcactcc attgcgccgc ttgcacctga
    40021 cctgctgacc attaccttta acggactgtc gaaaacgtac cgcgttgcag gcttccgtca
    40081 ggggtggatg gtgttgaacg ggccgaaaaa acacgctaaa ggttacatcg aaggtctgga
    40141 aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc
    40201 actgggtggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca
    40261 gcgtaaccgc gcgtgggaat tgatcaacga tattccgggc gtttcctgcg tgaaacctcg
    40321 tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc tttaacattc acgacgatca
    40381 gaaaatggtg ctggatttcc tgttgcagga aaaagtcctg ttggtgcaag ggacggcatt
    40441 caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga
    40501 gctgtctttg agtaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt
    40561 tcactgccgg agattgcatc cggcagtgtt atcccgccac aatgacctga tgatgtcatc
    40621 atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc
    40681 attaaccgct ggccgctaat gcgcaacgtg cggacggaaa atgtgtccga acacagtttg
    40741 caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcgtaaatt tggcggtaat
    40801 gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcttcaga agtgctcacc
    40861 ggagatctcc ctactccggt gaaatacttc aattcgcaaa tcgctcagga atacaaggct
    40921 attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct acaggatatc
    40981 tttgcgccgt taattgacga gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag
    41041 gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa
    41101 ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag ccaggagatg
    41161 gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga gattagccag
    41221 gattcaccgc tgtaagcagc tggagtctgc gtcgcatcag gcaataagcg ccggatgcga
    41281 catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca
    41341 cgataatggt gaacggtacc cccaacttaa caaagtcact gaagctgtaa ttccccggac
    41401 ctaaaaccag tgtgttaaca ggtgaagata tcggtgtcat aaaggcggcg gatgctgcca
    41461 tcgccacgac catcgcaaat ggataaggcg acacccccat cgttttggca gcagccagcg
    41521 caatcggagc catcaacacc gccgtcgcag tattagaaat aaatagccca ataaccgccg
    41581 acaagacaaa caaacaaccc agcatcatat gtggcccata accgccgcca atgtccatca
    41641 gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca aacggcatca
    41701 tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcactt tcggcatcga
    41761 tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt
    41821 catcggtcag cattaacgcc accattagca ccagacagaa aatggcatgg ggtgcctggc
    41881 tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgccacg aagtcgcggc
    41941 cctgtttggc cagcataccg atcagtttcc agttaccaac aaccagaatg atatcgccca
    42001 gcagcagagg ctcatccgcc agcgaacctt ccagcgccac accattgcgc tttagcccca
    42061 ccacattcaa tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaataagtt
    42121 ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat
    42181 actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga
    42241 catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat
    42301 taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcac
    42361 cataacgttc acgtaatttg agatcgtcca gtcgttgacc aatcattggc gatccggggc
    42421 gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg
    42481 ttcggcgcgt ccagccttcg cgttgtgggg tctgggtatc ccctttcagc atgaaacgca
    42541 tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa
    42601 agaaactgaa gccgtgatag ccttcacgca gtaattcact gttgactacc aggttcggcg
    42661 gcgtcgccac cagcgtcatc atgccgctta tcagcccggc aaaacttaac ggcatcatca
    42721 gacgcgacgg cgacgtttgc atacgcatgg caacgcttaa caccacgggg ataaagatag
    42781 cgacaacgcc ggttgaactc ataaacgcgc caagtcccgc gacggtcagc atcaacaaaa
    42841 ccaacatttt gatttcactg ttgcccgcca ctttgaccag ccatgtcccc attacagtgg
    42901 caacaccggt acggaccaaa ccatcgccaa taatgaacaa ggcggcaatc aggacaacgt
    42961 taggatcgga aaagccggaa aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg
    43021 cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacga
    43081 tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt
    43141 aattgagggg gataacttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca
    43201 tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg
    43261 ctccagactg tggaggacta aatcgacttc attcaggcgc ggggtatctg ccggggcatt
    43321 aaccgcaata acatggcaac ccgccgccag acctgaaagc acgccagcgg gagcatcttc
    43381 caccacaaca cactcctgcg gcgcaagccc cagcagctgc gcgcctaaca gatacgcatc
    43441 aggttctggt tttccgcgct tcacccgctc agcggttaca aacacctctg gcgcgggaag
    43501 ccccgctatt ttatgacgcg ctcgcgctac cggcatggag ccagaagtca caatagccca
    43561 cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa gcgcggtaat
    43621 accttcggtt tccgtggcct cgatgtgttc cagacgcgta aactcggcgg caatgtctgc
    43681 ctcggatttg cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa
    43741 agccagcacc tcttccggcg ataacccatg acgtctggcc cagttgctcc acgcccgttc
    43801 taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg
    43861 cacgcgggcc tcctcaggcg ttaataattt gattaatttc gttggcgctc aaatggtact
    43921 gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct
    43981 gggcgttaaa accgtgagtt ccagcgtcaa aatgggtata gcgcccttcc acattaacca
    44041 taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga
    44101 cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat
    44161 gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact
    44221 cgcgatccag ttcacgcatc tgtaatccat aaccacgctc aatgattgtt tgcaggcgac
    44281 ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactgg ttagacaaaa
    44341 taagacgttg cgcgttggtc atttccatta ttgactcctg tatcactcta ctacggtgaa
    44401 aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca
    44461 tcaaggaaag ttttatccag ttgtttgaag gcacgcttaa gcgtgtcagc taatgcctgg
    44521 taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga
    44581 acttcataaa accagttgag gattgcagga ggtaatggcg ttacagaacg cttgcccagc
    44641 caccacaatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac tgccggccca
    44701 agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa
    44761 cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagactgg ggcaaggcgt
    44821 ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta
    44881 acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa
    44941 atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca
    45001 ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa
    45061 attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt
    45121 tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat
    45181 catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact
    45241 tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc
    45301 atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg
    45361 cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca
    45421 ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca
    45481 gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat
    45541 accagctccg tagtgattga tgagtctgtt attcagggta tcaaagatgc agcttctttt
    45601 gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca
    45661 cagctgaaag acaaaaacgt tgctgtattt gacaccgcat tccaccagac tatgccggaa
    45721 gagtcttatc tctacgccct gccgtacaac ctgtacaaag agcacggcat ccgtcgttac
    45781 ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa
    45841 ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct
    45901 atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc
    45961 atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg
    46021 ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg
    46081 accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg
    46141 aagcgcgcaa tggacgttta ctgccaccgc ctggccaaat acatcggtgc ctacactgcg
    46201 ctgatggatg gtcgtctgga cgctgttgta ttcaccggtg gtatcggtga aaatgccgcg
    46261 atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa
    46321 cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct
    46381 gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact
    46441 gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg
    46501 gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg
    46561 tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc
    46621 tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta
    46681 cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct
    46741 acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag atcatcgcga
    46801 actaccacgc taacaccaaa gacgctgaag tcgttctggt ggaaggtctg gtcccgacac
    46861 gtaagcacca gtttgcccag tctctgaact acgaaatcgc caaaacgctg aacgcagaaa
    46921 tcgtcttcgt tatgtctcag ggcactgata ctccggaaca gttgaaagag cgtatcgaac
    46981 tgactcgcaa cagcttcggc ggtgcaaaaa acaccaatat taccggcgtt atcgttaaca
    47041 aactgaacgc tccggttgat gagcagggtc gtacccgtcc ggatctgtcc gagatttttg
    47101 acgactccac caaagcaaaa gtgaacaacg ttgatccggc gaagctgcaa gaatccagcc
    47161 cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg
    47221 atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg
    47281 ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg
    47341 gttctctgct ggtgacttcc gcagaccgcc ctgacgtgct ggttgccgct tgcctggctg
    47401 ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc
    47461 gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca
    47521 ccaacacctg gcagacttct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg
    47581 atcacgagcg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga
    47641 tcgattctct gactgccact tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt
    47701 atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg
    47761 aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac
    47821 tgctgggtaa tccggcagag atcaaccgtg ttgcagcctc tcagggtgta gaactgggtg
    47881 caggcattga aatcgttgat ccagaagtgg ttcgcgaaaa ctatgttggt cgtctggtcg
    47941 aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg
    48001 tggttctcgg tacgctgatg ctggaacaag atgaagttga tggtctggtt tccggtgctg
    48061 ttcacaccac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca
    48121 gctccctggt atcttccgtg ttcttcatgc tgttgccgga acaggtttac gtttacggtg
    48181 actgtgcgat caacccggat ccgaccgcag aacagctggc agaaatcgcg attcagtccg
    48241 ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg
    48301 gtacttctgg tgctggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg
    48361 aaaaacgtcc tgatctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg
    48421 acgttgcgaa atccaaagca ccgaactctc cggttgcagg tcgcgctacc gtgttcatct
    48481 tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct gctgacctga
    48541 tctctatcgg accgatgctg cagggcatgc gcaagccggt taacgacctg tcccgtggcg
    48601 cactggttga tgatatcgtc tacaccatcg cgctgactgc aattcagtct gcacagcagc
    48661 agtaatctcg tcatcatccg cagctttgcg ctgcg
//
LOCUS       DAOFSV010000020        47819 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.020, whole genome shotgun sequence.
ACCESSION   DAOFSV010000020 DAOFSV010000000
VERSION     DAOFSV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.020"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(32..508)
                     /gene="greA"
                     /locus_tag="QBH27_001243"
     CDS             complement(32..508)
                     /gene="greA"
                     /locus_tag="QBH27_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDQ7013579.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            756..2189
                     /gene="dacB"
                     /locus_tag="QBH27_001244"
     CDS             756..2189
                     /gene="dacB"
                     /locus_tag="QBH27_001244"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDQ7013580.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGIAWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(2230..3402)
                     /gene="cgtA"
                     /locus_tag="QBH27_001245"
     CDS             complement(2230..3402)
                     /gene="cgtA"
                     /locus_tag="QBH27_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDQ7013581.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(3418..4383)
                     /locus_tag="QBH27_001246"
     CDS             complement(3418..4383)
                     /locus_tag="QBH27_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ7013582.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(4510..4767)
                     /gene="rpmA"
                     /locus_tag="QBH27_001247"
     CDS             complement(4510..4767)
                     /gene="rpmA"
                     /locus_tag="QBH27_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDQ7013583.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(4788..5099)
                     /gene="rplU"
                     /locus_tag="QBH27_001248"
     CDS             complement(4788..5099)
                     /gene="rplU"
                     /locus_tag="QBH27_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDQ7013584.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            5358..6329
                     /gene="ispB"
                     /locus_tag="QBH27_001249"
     CDS             5358..6329
                     /gene="ispB"
                     /locus_tag="QBH27_001249"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDQ7013585.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            6562..6840
                     /gene="sfsB"
                     /locus_tag="QBH27_001250"
     CDS             6562..6840
                     /gene="sfsB"
                     /locus_tag="QBH27_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDQ7013586.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(6888..8147)
                     /gene="murA"
                     /locus_tag="QBH27_001251"
     CDS             complement(6888..8147)
                     /gene="murA"
                     /locus_tag="QBH27_001251"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDQ7013587.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(8202..8456)
                     /gene="ibaG"
                     /locus_tag="QBH27_001252"
     CDS             complement(8202..8456)
                     /gene="ibaG"
                     /locus_tag="QBH27_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDQ7013588.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(8616..8909)
                     /gene="mlaB"
                     /locus_tag="QBH27_001253"
     CDS             complement(8616..8909)
                     /gene="mlaB"
                     /locus_tag="QBH27_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDQ7013589.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(8909..9544)
                     /gene="mlaC"
                     /locus_tag="QBH27_001254"
     CDS             complement(8909..9544)
                     /gene="mlaC"
                     /locus_tag="QBH27_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDQ7013590.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(9563..10114)
                     /gene="mlaD"
                     /locus_tag="QBH27_001255"
     CDS             complement(9563..10114)
                     /gene="mlaD"
                     /locus_tag="QBH27_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDQ7013591.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(10119..10901)
                     /gene="mlaE"
                     /locus_tag="QBH27_001256"
     CDS             complement(10119..10901)
                     /gene="mlaE"
                     /locus_tag="QBH27_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDQ7013592.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(10909..11718)
                     /gene="mlaF"
                     /locus_tag="QBH27_001257"
     CDS             complement(10909..11718)
                     /gene="mlaF"
                     /locus_tag="QBH27_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDQ7013593.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            11928..12905
                     /locus_tag="QBH27_001258"
     CDS             11928..12905
                     /locus_tag="QBH27_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDQ7013594.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            12919..13905
                     /gene="kdsD"
                     /locus_tag="QBH27_001259"
     CDS             12919..13905
                     /gene="kdsD"
                     /locus_tag="QBH27_001259"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDQ7013595.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            13926..14492
                     /gene="kdsC"
                     /locus_tag="QBH27_001260"
     CDS             13926..14492
                     /gene="kdsC"
                     /locus_tag="QBH27_001260"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDQ7013596.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            14489..15064
                     /gene="lptC"
                     /locus_tag="QBH27_001261"
     CDS             14489..15064
                     /gene="lptC"
                     /locus_tag="QBH27_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDQ7013597.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            15033..15590
                     /gene="lptA"
                     /locus_tag="QBH27_001262"
     CDS             15033..15590
                     /gene="lptA"
                     /locus_tag="QBH27_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDQ7013598.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            15597..16322
                     /gene="lptB"
                     /locus_tag="QBH27_001263"
     CDS             15597..16322
                     /gene="lptB"
                     /locus_tag="QBH27_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013599.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            16370..17803
                     /gene="rpoN"
                     /locus_tag="QBH27_001264"
     CDS             16370..17803
                     /gene="rpoN"
                     /locus_tag="QBH27_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDQ7013600.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            17826..18113
                     /gene="hpf"
                     /locus_tag="QBH27_001265"
     CDS             17826..18113
                     /gene="hpf"
                     /locus_tag="QBH27_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDQ7013601.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            18231..18722
                     /gene="ptsN"
                     /locus_tag="QBH27_001266"
     CDS             18231..18722
                     /gene="ptsN"
                     /locus_tag="QBH27_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDQ7013602.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            18768..19622
                     /gene="rapZ"
                     /locus_tag="QBH27_001267"
     CDS             18768..19622
                     /gene="rapZ"
                     /locus_tag="QBH27_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDQ7013603.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            19619..19891
                     /gene="npr"
                     /locus_tag="QBH27_001268"
     CDS             19619..19891
                     /gene="npr"
                     /locus_tag="QBH27_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDQ7013604.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            20104..20736
                     /gene="yrbL"
                     /locus_tag="QBH27_001269"
     CDS             20104..20736
                     /gene="yrbL"
                     /locus_tag="QBH27_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDQ7013605.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(20733..21461)
                     /gene="mtgA"
                     /locus_tag="QBH27_001270"
     CDS             complement(20733..21461)
                     /gene="mtgA"
                     /locus_tag="QBH27_001270"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDQ7013606.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(21458..22111)
                     /gene="elbB"
                     /locus_tag="QBH27_001271"
     CDS             complement(21458..22111)
                     /gene="elbB"
                     /locus_tag="QBH27_001271"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDQ7013607.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(22341..24677)
                     /gene="arcB"
                     /locus_tag="QBH27_001272"
     CDS             complement(22341..24677)
                     /gene="arcB"
                     /locus_tag="QBH27_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDQ7013608.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(24773..25702)
                     /locus_tag="QBH27_001273"
     CDS             complement(24773..25702)
                     /locus_tag="QBH27_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDQ7013609.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            26377..30837
                     /gene="gltB"
                     /locus_tag="QBH27_001274"
     CDS             26377..30837
                     /gene="gltB"
                     /locus_tag="QBH27_001274"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDQ7013610.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            30850..32268
                     /gene="gltD"
                     /locus_tag="QBH27_001275"
     CDS             30850..32268
                     /gene="gltD"
                     /locus_tag="QBH27_001275"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDQ7013611.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(32336..32800)
                     /locus_tag="QBH27_001276"
     CDS             complement(32336..32800)
                     /locus_tag="QBH27_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ7013612.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(32797..33672)
                     /gene="nanK"
                     /locus_tag="QBH27_001277"
     CDS             complement(32797..33672)
                     /gene="nanK"
                     /locus_tag="QBH27_001277"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDQ7013613.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(33669..34358)
                     /locus_tag="QBH27_001278"
     CDS             complement(33669..34358)
                     /locus_tag="QBH27_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDQ7013614.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(34406..35896)
                     /gene="nanT"
                     /locus_tag="QBH27_001279"
     CDS             complement(34406..35896)
                     /gene="nanT"
                     /locus_tag="QBH27_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDQ7013615.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSCVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(36005..36898)
                     /gene="nanA"
                     /locus_tag="QBH27_001280"
     CDS             complement(36005..36898)
                     /gene="nanA"
                     /locus_tag="QBH27_001280"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDQ7013616.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(37020..37811)
                     /gene="nanR"
                     /locus_tag="QBH27_001281"
     CDS             complement(37020..37811)
                     /gene="nanR"
                     /locus_tag="QBH27_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDQ7013617.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(37919..38416)
                     /gene="sspB"
                     /locus_tag="QBH27_001282"
     CDS             complement(37919..38416)
                     /gene="sspB"
                     /locus_tag="QBH27_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDQ7013618.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(38422..39060)
                     /gene="sspA"
                     /locus_tag="QBH27_001283"
     CDS             complement(38422..39060)
                     /gene="sspA"
                     /locus_tag="QBH27_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDQ7013619.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(39455..39847)
                     /gene="rpsI"
                     /locus_tag="QBH27_001284"
     CDS             complement(39455..39847)
                     /gene="rpsI"
                     /locus_tag="QBH27_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDQ7013620.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(39863..40291)
                     /gene="rplM"
                     /locus_tag="QBH27_001285"
     CDS             complement(39863..40291)
                     /gene="rplM"
                     /locus_tag="QBH27_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDQ7013621.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(40511..41638)
                     /gene="zapE"
                     /locus_tag="QBH27_001286"
     CDS             complement(40511..41638)
                     /gene="zapE"
                     /locus_tag="QBH27_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDQ7013622.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYKELVNSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            41832..42230
                     /locus_tag="QBH27_001287"
     CDS             41832..42230
                     /locus_tag="QBH27_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDQ7013623.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            42384..43751
                     /gene="degQ"
                     /locus_tag="QBH27_001288"
     CDS             42384..43751
                     /gene="degQ"
                     /locus_tag="QBH27_001288"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDQ7013624.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            43841..44908
                     /gene="degS"
                     /locus_tag="QBH27_001289"
     CDS             43841..44908
                     /gene="degS"
                     /locus_tag="QBH27_001289"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDQ7013625.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(44971..45909)
                     /gene="mdh"
                     /locus_tag="QBH27_001290"
     CDS             complement(44971..45909)
                     /gene="mdh"
                     /locus_tag="QBH27_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDQ7013626.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            46344..46814
                     /gene="argR"
                     /locus_tag="QBH27_001291"
     CDS             46344..46814
                     /gene="argR"
                     /locus_tag="QBH27_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDQ7013627.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
     gene            47178..47441
                     /gene="yhcN"
                     /locus_tag="QBH27_001292"
     CDS             47178..47441
                     /gene="yhcN"
                     /locus_tag="QBH27_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDQ7013628.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(47496..47768)
                     /locus_tag="QBH27_001293"
     CDS             complement(47496..47768)
                     /locus_tag="QBH27_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDQ7013629.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
BASE COUNT        11819 a        12834 c        11958 g        11208 t
ORIGIN      
        1 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
       61 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
      121 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
      181 cgccaacgat gcgataagtc tgttcttcgt cagaatcgag attcagcacc gttacggtag
      241 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg
      301 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
      361 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
      421 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
      481 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
      541 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
      601 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatagt
      661 cattttaccc tggagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
      721 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
      781 gattgaccag ctgtatagcg ttcagtgttc aggccgcaaa tgttgatgag tacattactc
      841 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg
      901 ctattgatta ccacagtcag cagatggcgc tacccgccag tacgcagaaa gtgattactg
      961 cgctggcggc gttgattcaa ctcggccccg attttcgttt taccacgacg cttgaaacca
     1021 aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt tgcgcgattt ggtgccgatc
     1081 cgacgttaaa acgtcaggat attcgcaata tggtcacgac tttgaaaaaa tctggcgtca
     1141 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
     1201 ccggctggcc atggaatgac atgacacaat gctttagcgc accgcctgcc gccgccatag
     1261 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaaacctggt gatatggctt
     1321 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
     1381 gtggttctgc cgaagcgcaa tactgcgaac tggatgtcgt accaggtgac ctgaaccgct
     1441 ttacgctaac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc
     1501 aggatggtgc cagctatgcc ggggcaattc tgaaagatga gttaaaacag gcgggtatcg
     1561 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
     1621 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
     1681 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
     1741 ggcgggccgg atcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatatcg
     1801 gaaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
     1861 ccatgatgca ggtgctgcaa tatattgccc aacacgacaa tgaacttaac tttatctcca
     1921 tgctgccgct ggcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
     1981 gcgtggatgg aaaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
     2041 gattcattac cacagcgagc gggcaacgaa tggcgtttgt gcaatatctt tctggctatg
     2101 cagtagaacc ggcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
     2161 tgtataaaga tatttatcag aataattagc ctaaagagaa accccggcac atggccgggg
     2221 ttttgctgat taacgcttgt aaatgaactc aacgccttct tcgtcgtctt cgtcccagtc
     2281 gtcatcccag tcctcatcat cctcttcagc aatctcttca agctgctgac ggtgataatc
     2341 atcccacatg aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt
     2401 ttcaatgata aaggtcatca catcccagca gagatctttc acgcccagtc cgctcgccgc
     2461 agagatcaga taatatttat cttcccagcc cagcgcttca gcgatcgctt tcgctttctc
     2521 ttcggcttct gccttatcca gcagatcgat cttgttgaaa actaaccaac gcggtttcgc
     2581 cgccagatcc tggctgtatt tttccagctc actgataata atacgcgcgt tttcaaccgg
     2641 atcggtgccg tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag
     2701 gtgcttcagg aagcgaatgc ccagacctgc gccttctgca gcgccttcaa tcagccctgg
     2761 aatatcggca acaacgaagc tcttttcgtt gtccattcgc accacgccca ggcttggtac
     2821 cagagtggta aacggataat ccgccacttt cggtttagcc gccgatacag cacgaataaa
     2881 ggtcgattta cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc
     2941 cagcagcagc tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt
     3001 aactgacgat ttgaaacgag tgttgcccag accgtgccag ccgcccttag caaccagcag
     3061 acgctgaccg tgtttggtca tatcacccat ggtttcacca gtaccctggt cgattacacg
     3121 cgtacctacc ggcactttaa tcgtgacgtc tttaccgcgc ttacctgtac agtcgcggct
     3181 tgcaccgttt tgaccgcgct ctgcacggaa agatttttca aaacgataat cgataagcgt
     3241 gttcaggttc tcgtcggctt ccatccatac gtcaccaccg tcaccgccgt cgccgccatc
     3301 cgggccgcct ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc
     3361 acctgcaacg accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca
     3421 ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg
     3481 atgaccaatg gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa
     3541 aaggtttaac atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa
     3601 aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg
     3661 agccatcgct tccgccaggg cgccatatcc taccaatgta ttcagtccgc aaaaaattaa
     3721 acaggcgagc tgccagtggc taagctgcgc tatcactcca ggatttgcca gagggaagag
     3781 cgcaattgta cataaagtgt acagcaaaaa caggatctgc ggtgaggcca gccgacgcaa
     3841 taaaaccttt tgcgccacgc cataactcac ccaaaccgtc gccgcaccga ccccaaagat
     3901 aactccccag gtgtaatcgg tgagctttgt aaatatctcg accagactgg tgttaaaaaa
     3961 catcaccagg ccgctcagga gcatcaatgc ccctacaacc tgagtactgc gcattttctc
     4021 tttcaggata aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg
     4081 cgaagcggtc ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag
     4141 cccggcggtc gccactgcta acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg
     4201 cggcaaccgc ttcttcaccg caagaatggc accaaggcca atactcgcca tcaagaaacg
     4261 gtaaaacacg attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa
     4321 cgccccccag caaattgctg tggtgagcgc caaaagaatg ccaatgcctg cctgctgctt
     4381 cataccgtat tccctgcaaa gcgcatatgc ggattaacgt cgcaccatgc gcatttttac
     4441 cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac
     4501 gcgaaaaact tattcagctt cgatgctgat aaatttacgg tttttcgggc ctttaacttc
     4561 gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt
     4621 agcgccagcg tggaatttgg taccacgttg acgaacgatg atgctacccg ccagaacgga
     4681 ttcaccaccg aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt
     4741 ggagccgcca gcctttttat gtgccatttg aaatctctcc tcaggtctta ggcgctgatg
     4801 ccagtaattt tcacatcagt gaaccactga cgatggccct gctgcttacg atagtgttta
     4861 cgacgacgaa acttaacgat tttaactttc tcgccacgac cgtgagcaac aacttcagct
     4921 ttgattacgc cgccatcaac gaaaggaacg ccgattttga cttcttcacc gtttgcgatc
     4981 atcagcactt cagcgaactc aacagtttcg ccagttgcga tgtccagctt ttccaggcga
     5041 acggtctgac cttcgcttac tcggtgttgt ttaccaccac tttggaaaac cgcgtacata
     5101 taaaactccg cttccgcgca caccttttca atgattcaga gtgcgctata aatattcaca
     5161 atagggcgcg aatattacgc aaaacgcacg cctttgacaa gtgctacagt caatacacga
     5221 agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc cattttttca gtacaatcac
     5281 cctatattcc taaccataaa ccctaagttg cctttgttca cagtaaggta atcggggcga
     5341 aaagcccggc ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg caagatatgg
     5401 cgggtgttaa tgcggcaatc cttgagcagc ttaattccga cgtccaactg atcaatcagt
     5461 taggctatta catcgtcagc ggcggcggta aacgtattcg tccgatgatt gctgtactgg
     5521 ctgcacgagc tgttggctat gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt
     5581 ttatccacac ggcgactctg ctacacgacg acgttgtgga tgaatcagat atgcgcaggg
     5641 gtaaagctac cgccaacgcc gcatttggca atgccgccag cgtgctggta ggcgatttta
     5701 tttatacccg cgcgttccag atgatgacca gcctcggttc actcaaagtg ctggaagtca
     5761 tgtcagaagc cgtaaacgtc atcgcagaag gtgaagttct gcaactgatg aacgttaacg
     5821 atccggacat cactgaagaa aactacatgc gcgttatcta cagcaagact gcgcgtctgt
     5881 ttgaggctgc cgcgcagtgt tccgggattc tggctggctg tacgccggag gaggagaaag
     5941 gcctacaaga ttatgggcgc tatctcggca ctgctttcca gttgatcgac gatttactcg
     6001 attacaatgc cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg aacgaaggta
     6061 aaccgacgct gccgctgctg catgcgatgc atcatggcac accagaacag gcacagatga
     6121 ttcgtaccgc catcgaacag ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa
     6181 tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc cgaggaagaa gcagacaaag
     6241 ccatcgcagc gttacaggtg ctcccggaca ccccgtggag agaagcactt atcggcctcg
     6301 cgcacatcgc tgttcaacgc gatcgttaat cccctcccct catcccgcgc agtgcgcggg
     6361 atgattccag taaattccat aaaacactta ttcagctcta taactcctga aaaacagggc
     6421 tgtgtatctt tatatattct gaatattcac actctttaca ggaacttttt agagcaatag
     6481 gccatcagga gtatagtgat gctcgacaga aacagaagaa gtattctgaa tgaaagcgac
     6541 taatttaagg agtgaggaaa gatggaaagt aatttcattg actggcatcc cgctgacatc
     6601 attgcaggtt tgcgcaagaa gggaacgtca atggcggcgg agtctcgcag aaatggtttg
     6661 agttcctcaa cgctggcgaa cgcattatca cgcccatggc cgaaaggaga gatgattatt
     6721 gcgaaagccc tgggaactga cccctgggtt atctggccat cacgctacca tgatccgcag
     6781 acccatgagt ttatcgacag aacgcagttg atgcgtagct acactaaacc gaaaaaatga
     6841 gtggtctggc ggtagccccg cgaacggggc tgccagctct cagacaatta ctcgcctttc
     6901 acacgctcaa tatttgcacc taaagcgcgt agtttgtctt caatgcgttc gtagccacga
     6961 tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg caatacagcc agccagcacc
     7021 aggcttgctg atgcacgcag atcggttgcc ataacctgtg cgccagaaag tttttcaaca
     7081 ccgtgacaaa taacggtatt gctttcgatt tcggcgtgcg cgcccatacg gctcagctct
     7141 ggcacatgca taaagcggtt ttcaaagacc gtttcggtga tgaatccggt cccttctgcc
     7201 accaggttca acagcgtgaa ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg
     7261 gtacgtacgt taacagcctt cggacgtttg ccatgcatat ccaggctaat ccagtcttcg
     7321 ccgacttcga tgtccgctcc agcgtcacgc agtttcgcca gcacggcgtc cagagtatct
     7381 ggctgcgcgt tacggcagat aattttgccg cgagagatcg ccgccgccac caggaaagta
     7441 ccggtttcga tacgatccgg cagcacgcga tagacaccgc cgcctaaacg ttccacacct
     7501 tcgatgacga tacgatcggt gccctgaccg ctaattttcg cacccagcgt aatcaggaag
     7561 ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt caataatcgt ggtgccttcc
     7621 gccagggttg cagcacacat gatggtcacc gttgcgccaa cgctgacttt atccatcacg
     7681 atatgtgcgc ctttcagacg accatcgacg gaagctttaa cgtaaccttc ttccagtttg
     7741 atggtcgcgc ctaattgttc gaggccagaa atgtgtagat caaccggacg tgcgccgatc
     7801 gtacaaccgc caggcagtga aacttgcccc tgaccaaagc gcgctaccag cggccccagc
     7861 gcccagatag aagcacgcat ggttttaacc agatcgtaag gtgcgcagaa tacattaacg
     7921 tcgcgggcat caatatgcac agaaccatta cgttctactt tcgcacccag ctggcttagc
     7981 agcttcattg atgtatcgac gtcttttagt ttcgggacgt tctggatctc taccggttct
     8041 tccgccagta gtgcggcaaa aaggataggc agagcagcat ttttagcgcc ggaaattgtg
     8101 acttcgccct ggagcttcgt tggcccctga acacgaaatt tatccattta gtttgttctc
     8161 agttaacaat tcatatccgc taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg
     8221 cgatcgcgcg cccactccgc aggggtatac gctttgatcg acacagcatg aatgcggtta
     8281 tccgcaatat attccatcag cgggccatag accgtctgct gttttttaac ccgactcatg
     8341 ccgtcaaaca actcacccac ggcaataacc tgaaagtggc tgccatcgcc ggaaacgtgg
     8401 acttcctgga gggagagagc gttcatcaac acgctctgaa tttcattatt ttccatggga
     8461 tcttcaatca tcagttaata aatcagcgaa acatcttaga gcaaagttgc gctggcataa
     8521 ataagcaaaa agcctcgctg ataaatcaga caaggctcga cttgcaggca ggtttgccgg
     8581 acaggcggtt aacgccatat ccggcctgaa aaaatttaac gaggcagaac atcagcaggc
     8641 aaattataca atttcgccag ggtatacact ttgtcgttta ccccctgaag cgtcacattg
     8701 ttgccctgct ttttcgccag atcgataaga tggagcagca gtgccagtcc acccgtatcc
     8761 acgcgagaca cacggctaag atcgatgctg gtaatcccct tcaccgcttc ctcacgtatt
     8821 tcccaaagcg gtagcaaaac gtcctgatcc agctctccgg ataacgccag cgtgtcaccc
     8881 gtctgcatcc agctcagtga ctcgctcatt attttttctc ttccagagtg attttctgtt
     8941 gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc
     9001 cccactcgtt ttgtttggtg gtgatcatac tgacgccttc agcaatcatg tcgtaagcct
     9061 gccagttgcc cgtctgggag tttttacgcc actggaagtc cagacgcacc ggcgggcggc
     9121 cattcgggtc aataatggta acgcgaatcg gcacaatggt tttatcaccc agcggctgtt
     9181 ctggcgcaat ctgataggtt tgaccgtgat acatcgccag cgcctgaccg taagcctgct
     9241 tcaggtactc acggaaagcg gcaaagtaag catcacgttg agctggggtc gcactcttgt
     9301 aatactggcc cagcaccagc gcaccggcgt atttcacctg tacgtatggc aacagttcct
     9361 gatcaacaat ggtacgcaga taatccgggt tggcccgaat ttgcggttgc tcattcttca
     9421 ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt atacggattg gtctggtctg
     9481 ccgcggttgc cgcactcagc ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa
     9541 acatgcgtcg gttctcctga aattatttcg ttgtacccac aggttcagtg gtttcattat
     9601 tacctggcgc agcagctggc gcatcgccac tattcttatt gtcatcgcct ttactaccgt
     9661 aaaggaactg accaatgaga tcttccagca ccatcgcaga cttggtgtcc tgaattgtat
     9721 cgccatcctt caggatagca gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca
     9781 gatattgttc ccccagcagg ccggaagtac gaatgctcag cgaactggta tcaggaatgt
     9841 ggttataacg ttgttcaatt tccagcgtta cgcgcggcag ataggttttc gggtccagcg
     9901 taatatccgc cacccggccc acaacaacgc caccaatgct gaccggagag cgggctttca
     9961 ggccgccaat gttatcgaac gtcgcataaa gcgtataggt cggttcagta cgtatagacg
    10021 tcacgttcgc tgccttcagg caaacaaaca gcgccgcaag cagtgctgct aataaaaaga
    10081 tacccaccca aatttcattt tttttcgttt gcatgaactc aattcccaaa catcaatgcg
    10141 gtcagcacaa aatccagccc cagaacagcc agagacgagt ggacaacggt gcgagtggtt
    10201 gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac
    10261 gtcaccgtga tggcgaacac cacgctctta atcagacagt tgacgagatc catacgccag
    10321 tcgacggcat tttgcattgc cgaccagaag aacccactat caatgccttt ccaactgacg
    10381 ccgaccagcg atccgcccca gatccccacg gcgacgaaga taaccgtcaa cagtggtaat
    10441 gaaataactc cagcccagaa acggggagaa ataacccgac gcagcggatc caccgccatc
    10501 atctccatac tggagagttg ctctgtagcg cgcatcaggc cgatttctgc ggttagcgcc
    10561 gaaccagcac gcccggcaaa caacaacgcg gcaaccaccg gccccagttc acgcagtagc
    10621 gataacgcca ccagcatacc cagactggtt tccgcactat aagtggtcag aaccagataa
    10681 ccttgcagcc ccaacaccat tccgatgaac acgccagaaa caacaataat cagcatcgac
    10741 aggacgccga cattatagag ctggcgcacc agcagcggtg catgtttgcg aaattccggt
    10801 ttgccgacca gcgcattgaa taacattaac ccggcccgcc cgaacgttct cagggtttta
    10861 atccctttat gtccgagcga cgccagcgca tttaacagca tgagtggctt aactccctgg
    10921 taaaagatca gcgtgataat cgccggcagg atagcggaac ggaacaggcc cgtcagctat
    10981 cccgtccaga aactgacgta cgcgcggatc aggattcgcc tgcaacgcct gggcactacc
    11041 atgagcgaca atttttttgt ccgccaggat ccaggcgtga tccgcaatac ttaacacttc
    11101 cggcacatcg tgagaaacca ccacacaagt cacgcccagc gcgctgttca gctcagaaat
    11161 cagcttcacc agcacgccca tggtaatggg atcttgccca acaaagggct catcaaacat
    11221 gatgagatcc ggctccagcg caatcgcacg tgccagcgct gcacgccgcg ccatcccacc
    11281 ggaaagttca gaaggcataa gtttagccgc tccacgcagc cccacggcct ccagcttcat
    11341 catcaccgta ctatgcaaca atggcgcggg aagttgggta tgttcccgca gtggataggc
    11401 gacgttgtca aatacgttca tatcagtgaa caacgccccg gactgaaata acatgctcat
    11461 ccgtttgcgc actgtataca ggcgcgaacg agacatcgcc ggaatattct caccatcgaa
    11521 aagaatctca ccatgatctg gtgcgatttg cccgccaatc agacggagta gcgtcgtttt
    11581 accgatgccc gatggcccca tgatcgccgt gatcttccct cgcggcacgg tcagggaaat
    11641 attatcgaag atgcagcgat tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt
    11701 cgccacagac tgctccataa ttcacccttc gtcttgcgtt gattttctaa gcatggcgct
    11761 caatttaacc ttgaacccaa catatttaca gaatattacc tgccgtggtt agcgaaagct
    11821 ggcatttgtt ttacttttta gccgcataaa gtcaaaatta agaatctgtt acggctttct
    11881 gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg acgttagatg cttttagcta
    11941 cggcactgtt aattgttggt ttacttttgg tcgtttacag tgccgaccgc ctggtttttg
    12001 ccgcgtctat tctttgccga acctttggca tcccgccgtt gatcatcggc atgacggtgg
    12061 tcagtattgg tacatcgtta ccagaaatca tcgtctcgct tgccgcgtct ttgcacgaac
    12121 aacgcgattt agccgttggt acagccctcg gctcaaacat tatcaatata ttgctgatcc
    12181 tcggtctggc tgcgctggtt cgtcctttta ccgtccattc tgatgttcta cgccgtgaat
    12241 tacccttaat gttgttggtc agcgtggtgg ccggttccgt actctatgac ggacaactta
    12301 gtcgcagcga tggtatcttt ctcctctttc tggctgtgct atggctgctg ttcattgtta
    12361 aacttgcacg tcaggctgaa cgtcagggga ctgacagcct gaccagagag cagcttgcag
    12421 agctgccgcg tgaaggtgga ttgcccgtcg catttttatg gctcggcatc gcactgatca
    12481 ttatgccagt ggccacgcgg atggtagttg ataacgccac ggtgctggcg aattactttg
    12541 ccatcagcga gttgacgatg ggtctgacgg caattgctat cggaaccagc ctgccggaac
    12601 tggcaaccgc aatagcaggg gttcgcaaag gtgaaaacga cattgcggtc ggaaatatca
    12661 ttggcgcaaa catttttaat attgtcatcg tgttgggttt acccgcgctg ataacgccag
    12721 gagagattga tccactggcg tacagtcgtg actacagcgt gatgttgctg gtgagcatta
    12781 tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg ccgtggtgta ggggtattat
    12841 taactggcgg atttatcgta tggctggcga tgttgtactg gttatcgcca atactcgttg
    12901 aataactgga aacgcattat gtcgcacgta gagttacaac cgggttttga ctttcagcaa
    12961 gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg cggagcttga tcaatacatc
    13021 aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt gtaaagggaa agttgtcgtc
    13081 atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg cagcaacatt tgccagcact
    13141 ggtacacctt catttttcgt ccatcctggt gaagccgcgc atggtgattt aggcatggtc
    13201 accccgcagg atgtggtgat tgctatctct aactcaggtg aatccagcga aatcacggcc
    13261 ttaattccag tgcttaagcg tcttcacgta ccgttaatct gcatcaccgg tcgcccggag
    13321 agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt
    13381 ccgttagggc tggcaccgac cagcagcaca accgccacgc tggttatggg cgatgccctc
    13441 gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag attttgcgct ctcacaccca
    13501 ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg atattatgca tacgggcgat
    13561 gagatcccgc atgttaagaa aacggccagt ctgcgtgacg cattgctgga agttacccgc
    13621 aaaaatcttg gtatgactgt catttgcgat gacaatatga tgattgaagg catctttacc
    13681 gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg ttcgtcagtt aagtattgcc
    13741 gatgtgatga cgccgggggg aatacgcgtg cgcccaggca ttctggccgt tgaggcactg
    13801 aacttaatgc agtcccgcca tatcacctcc gtgatggttg ccgatggcga ccatttactc
    13861 ggtgtgttac atatgcatga tttactgcgt gcaggcgtag tgtaaagatt caaggataaa
    13921 caacaatgag caaagcaggt gcgtcgcttg cgacctgtta cggccctgtc agcgccgacg
    13981 ttatggcaaa agcagagaac attcgtctgc tgatcctcga cgttgacggc gtactgtcag
    14041 atggcctgat ttatatgggc aataacggcg aagagctgaa agcgttcaat gttcgtgacg
    14101 gttatggcat tcgttgtgcg ctcacctctg atattgaagt cgctatcatt accgggcgaa
    14161 aggctaaact ggtagaagat cgttgtgcca cattggggat cactcacttg tatcaggggc
    14221 agtcaaacaa actgatcgcc tttagcgatc tgctggaaaa actggcgatt gccccggaaa
    14281 atgtggctta tgtcggcgat gatctcatcg actggccggt aatggaaaaa gtgggtttaa
    14341 gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg cgccgattac gtaacgcgca
    14401 ttgctggcgg tcgtggcgca gtgcgagaag tttgcgactt attactcctg gcgcagggca
    14461 aactggatga agccaaaggg caatcgatat gagtaaagcc agacgttggg ttatcattgt
    14521 gctatcactg gcggttctgg tgatgatcgg cattaatatg gccgaaaaag acgataccgc
    14581 ccaggtggtc gtcaacaaca atgatcccac ctataaaagc gagcatacgg acacgctcgt
    14641 ctataaccca gaaggggcac taagctatcg attgattgct caacacgttg aatattattc
    14701 cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc acgtttgata aggataaaat
    14761 cccgacatgg tccgtaaaag cagataaagc caagctgacc aatgaccgga tgctctattt
    14821 atatggacac gttgaagtca atgcactcgt gccggactct caacttcgca gaatcacgac
    14881 ggataacgcg cagatcaatc tggtgacgca ggatgttacc tctgaagacc tcgtcacgtt
    14941 atacggaaca acatttaact ccagcggtct gaaaatgcgc ggcaacttac gcagcaagaa
    15001 cgccgagctg attgaaaagg ttagaacatc ctatgaaatt caaaacaaac aaactcagcc
    15061 ttaatcttgt gcttgccagc tcacttctgg ccgccagcat tccggcattt gccgtaaccg
    15121 gagacactga tcagccgatc cacattgaat cggaccagca atctcttgat atgcaaggca
    15181 acgtggttac ctttaccggt aatgttatcg tcacccaggg caccatcaaa attaatgccg
    15241 acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa agaagtgatt gacggctacg
    15301 gtaaaccggc aacgttctac cagatgcagg acaacggtaa acccgttgaa ggtcacgctt
    15361 cccagatgca ctacgaactg gcaaaagatt ttgtcgttct gacgggtaat gcttatctgc
    15421 agcaggtcga tagcaacatt aagggcgata agatcactta cctggtgaaa gagcagaaaa
    15481 tgcaggcttt cagcgacaaa ggcaagcgcg taaccaccgt tctggtgccg tcgcagttgc
    15541 aggacaaaaa caataaaggc cagaccccgg cacagaagaa gggtaattaa ttcgttatgg
    15601 caacattaac tgcaaagaac cttgcaaaag cctataaagg ccgtcgcgtg gtagaagacg
    15661 tcagcctgac cgtcaactcc ggggaaattg tcggtctgct ggggccaaac ggtgccggta
    15721 agaccaccac tttctacatg gttgttggca ttgtgccgcg cgatgcgggc aacatcatta
    15781 ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg cgcgcgccgc ggtatcggct
    15841 atctgccgca ggaagcctcc attttccgtc gcctcagcgt ttacgataac ctgatggcgg
    15901 tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga agaccgtgcg aacgagctga
    15961 tggaagagtt tcacattgag cacctgcgtg acagcatggg gcagtcactc tccgggggtg
    16021 aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa tccgaaattt attctgctcg
    16081 acgaaccgtt tgccggggtt gacccgatct cggttatcga cattaaacgc atcattgagc
    16141 acctgcgcga cagcggcctg ggcgtactga tcactgacca caacgtgcgt gaaacactgg
    16201 cggtttgtga acgcgcttat atcgtcagtc aggggcattt gatcgcccac ggcacgccta
    16261 cagaaatctt acaagacgaa cacgttaagc gtgtatacct tggggaagac ttcagactct
    16321 gatagggtag aagtttgcga cgttttagca ggagagtacg attctgaaca tgaagcaagg
    16381 tttgcaactc aggcttagcc aacaactggc gatgacgcca cagctccaac aggcaattcg
    16441 tctgttgcag ttgtcgacgc tggaacttca gcaagagcta cagcaggcgc tggagagcaa
    16501 tccgctgctt gagcaaatcg acactcatga agaaatcgac acccgcgaaa cgcaagacag
    16561 tgaaacactg gacaccgccg acgcgctcga acaaaaagag atgccggaag agctgccgct
    16621 cgatgccagt tgggacacca tttacaccgc tggtacacca tccggcacca gcggtgacta
    16681 cattgatgac gagctgccgg tctatcaggg cgaaacgacg cagaccttac aggattacct
    16741 gatgtggcag gtcgagttga caccgttttc cgacactgac cgcgcgattg ctacctctat
    16801 cgtcgatgcc gttgatgaca ccggttatct gactgtcccg ctggaagata ttctcgaaag
    16861 tatgggcgat gaagagattg acatcgacga agttgaagcc gtccttaagc ggatccaacg
    16921 gtttgatcca gtcggtgtgg cggcaaaaga tctgcgtgac tgcctgctga tccaactctc
    16981 ccaattcgat aagaccacgc catggctgga agaggccaga ctgatcatta gcgatcatct
    17041 cgatctatta gccaatcacg acttccgcac tttaatgcgc gtcacgcgtc tgaaagaaga
    17101 tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat ccgcgccccg ggcagtcgat
    17161 ccagactggc gaacctgagt atgtcattcc agatgtgctg gtgcgtaagc ataacggcca
    17221 ctggacggta gaactcaaca gtgacagcat tccgcgtctg caaatcaacc agcactacgc
    17281 ctcgatgtgc aataacgcgc gcaatgatgg tgatagccag tttatccgca gcaatctgca
    17341 ggatgccaaa tggttgatca agagtctgga aagccgtaac gatacgctac tgcgcgtgag
    17401 tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa ggtgaagaat atatgaaacc
    17461 gatggtactg gccgatatcg cccaggctgt cgaaatgcat gaatcgacga tatctcgcgt
    17521 gaccacgcaa aaatacctgc atagtccacg aggcattttt gaactgaaat atttcttttc
    17581 cagtcacgtc aatactgaag gcgggggtga agcctcttcc acagcgattc gtgcgctggt
    17641 gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg agcgacagca agttaacctc
    17701 tttgctgtcg gaacaaggta tcatggtggc acgccgcact gttgcgaagt accgagagtc
    17761 tttatccatt ccgccgtcaa accagcgtaa acagctcgtt tgacccaacc gataaggaag
    17821 acactatgca gctcaacatt accggaaata acgtcgagat caccgaggca ctgcgcgaat
    17881 ttgttacagc caaatttgcc aaacttgagc aatattttga ccgaatcaac caggtctatg
    17941 ttgttctgaa agtggagaaa gtcacccaca cctcagatgc aacactgcat gtaaacggtg
    18001 gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc tgccattgat ggcttaattg
    18061 ataagctggc acgccagttg accaaacata aagataaact gaaacaacac taattgtccg
    18121 ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa cgggccattt gtacggttaa
    18181 tgctccgaac ctgttccact gtttgagtgg gcaggttctt aggtgaaatt atgacaaata
    18241 atgatacaac tctacagctt agcagtgttc ttaacaggga atgtacgcga agccgcgtcc
    18301 actgccagag caaaaaacgc gccctggaaa tcatcagcga gctggcggcg aaacaactta
    18361 gcctggcgcc tcaggtggtt tttgaagcta tcctgacgcg ggaaaaaatg ggcagtaccg
    18421 gtatcggcaa tggtattgcc attccgcatg gcaaactgga agaagatact ctgcgtgccg
    18481 ttggcgtttt cgttcagctc gaaacaccta tagctttcga tgccatcgac aaccaaccgg
    18541 tggacttact ttttgccctg ctggtgccgg cagaccaaac taaaacgcac ctgcatactc
    18601 tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg ccgccgttta cgcgcagccc
    18661 agagcgatga agagctgtat caaatcatta cggataccga aggtactccg gatgaagcgt
    18721 agttattcgg taatgtctct tttagacgtt gtgaggagaa acagtacatg gtactgatga
    18781 tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct gcgtgcgctg gaagatatgg
    18841 gtttttactg cgtggataac cttcccgtag tgttgttacc cgatctggct cgaactctgg
    18901 ccgatcgaga gatttctgcc gccgtcagca ttgatgttcg taatatgccg gagtcaccag
    18961 aaatattcga acaggcgatg agtaacctgc ctgacgcttt ctcaccgcaa ctactgttcc
    19021 tggatgccga ccgtaatacc ttaattcgtc gttacagtga cacgcgccga ctgcatccgc
    19081 tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa agaaagcgat ttgctggagc
    19141 ctctgcgttc gcgagcggat ctgattgtcg atacctcaga aatgtccgtt cacgagctgg
    19201 cagaaatgct gcgtacccgt ctgttgggta aacgtgaacg tgaactgacc atggtctttg
    19261 agtctttcgg cttcaaacac ggtatcccta tcgatgcaga ttacgtcttt gacgtgcgct
    19321 tcttgccgaa cccacactgg gatccgaaac tgcgtccaat gacaggtctt gataaacctg
    19381 tcgccgcgtt cctcgaccgc cacacagaag tacacaattt tatctaccag acgcgaagct
    19441 atcttgagct atggttacct atgctggaaa ccaacaaccg tagctacctg acggtcgcca
    19501 ttggttgtac cggcgggaag caccgttcgg tgtatattgc agagcaactg gcagactact
    19561 tccgctcgcg cggtaaaaac gtccagtcac gccatcggac gctggaaaaa cgtaaaccat
    19621 gaccgtcaag caaactgttg aaattacaaa caagctgggc atgcatgccc ggcctgcaat
    19681 gaagctgttt gaattaatgc agggttttga cgctgaagtg ctgttacgta atgacgaagg
    19741 caccgaggct gaagccaaca gcgttattgc gctgctgatg ttggattctg ccaaaggacg
    19801 gcagattgaa gttgaagcga ccggtccaca ggaagaggaa gcactggccg ccgttatcgc
    19861 cctctttaac tctggttttg atgaagatta atcttcatca ctttgacatt caaatccctt
    19921 caagctcccc cggcaataag aaaagcgcgt actgaaatcg ttatcaatca acgtcgattg
    19981 tacatttcgt gcgcttttta ctttcagaag aaccttaaga aaaccttaag aagcattgtt
    20041 tagatttcgt ttaagttaat cgaccatact ggagatcgtc agaaaatatt tccaggagat
    20101 ggcatgattc gcttatctga acaaagtccc ctgggcaccg gaagacatcg gaaatgttat
    20161 gcgcatccgg aagatgccca acgctgtatc aagattgtct accatcgtgg cgatggtggc
    20221 gataaagaga tccgccgtga gttaaagtac tacgcgcatc ttggtcgccg gttaaaagac
    20281 tggagtggaa taccgcgcta tcacggtacc gttgagacgg attgcggaac cggttatgtc
    20341 tacgatgtga ttgccgattt cgacggcaag ccttccatta cgctgaccga atttgcagaa
    20401 caatgccgtt acgaagaaga cattgcacaa ctgcgccagt tactgaaaca gctgaagcgt
    20461 tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc cgcagaatat cctctgtcac
    20521 cgcatcagtg agtcagaggt tatccccgtc gtctgcgaca acattggtga aagcacattg
    20581 attccactgg cgacctggtc gaagtggtgt tgtctgcgta agcaagagcg gttatggaaa
    20641 cgatttatcg cccaaccggc gctggctatt gccctgcaga aagatctgca acctcgtgag
    20701 agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcaa gctggtgttg ttgcataaac
    20761 ggctcaccgc ctaactgata catctgccgt aaaatccacg cctgacggct acgcacatag
    20821 cccgatggcg cggagacttt gaaacgaagt ggattaggta atacagctgc cagtaatgca
    20881 gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat aacgttgtgc cgcagcttcg
    20941 acgccaaaca caccgtcgcc aaattcggcg atattcaggt aaaccgtcag gatacgcttt
    21001 ttgctccaga ctgtttctat ccccagcgtt aatccggctt ccagcccttt tcgaacccag
    21061 ctacgcccat cccataaaaa gagatttttg gctgtctgct gagaaatcgt tgaagcaccg
    21121 cgaatacggt tttcattgcg ctcgttgtgc gccagggctt gctcaatgga agcgacatca
    21181 aagccccagt gctcaggaaa tttctgatct tctgcggcaa tcaccgccag tcccatccac
    21241 ggcgagattt gatccatact gacccagtca gaatgtgcca cgtaacgaaa attgccatgc
    21301 agccaggcgc tgacttgccg ctcgaccatt accgctgaga aaggaacagg cgcaacgcta
    21361 aacaacgcga tgcccccgcc ccagaaaatg gcgaggacaa ccattaaccg cagaagaaaa
    21421 cggcgaacga aactaaacac cgttaagcgg cttttactca ttcagccaga accagcacgc
    21481 gggaaaccag cttatcaatg ccgctcgccg cttctgcaat gttctgcgcc agcatatatg
    21541 ccggggtggt gacaatctta ttgtcttcat caaccacgat atcatcgaca ggacacggca
    21601 catgctccgc gcccatctct tccagcactt ctgcggtatc gatatcagta ccgatggtca
    21661 aacgtagcgg gaaatcgaaa attttcggca gtatcgccgg tgcaatacac ataaaaccaa
    21721 gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc
    21781 attcgctacc aagactggca aaattgctta aattcttcgc cgcgccaaaa ccccccggca
    21841 caatcaacgc atccagttca gcggcatcgg cctgggccag aggacggatt tcaccgcgcg
    21901 ttattcgtgc cgcttcaatc agcacattgc gcgtttccgt catcgcttca ccagttaaat
    21961 ggttgataac atcaacctgc tgcttatccg gtgcaaagca aaccgcctgc gcaccgctgc
    22021 gtgaaatagc taacaacgtc aataccgctt catgaatttc agaaccgtca tagacgccgc
    22081 atccgctcag aattacgcca attttcttca ttgtgatctt cctttcgcaa ttgactgaaa
    22141 cacattaatt tttttaataa aaatgctacg catcacacat ttaactgatt catgtaacaa
    22201 atcatttaag ttttgctatc ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt
    22261 aactcatcat aataatttac ggcgcagcca agatttccct ggtgttggcg cagtattcgc
    22321 gcaccccggt ctagccgggg tcatttttta gtggcttttg ccacccacgc tttcagtact
    22381 tctacgtcgt gacgccactc ttctttcatc tcttcaatcc attcaccgac gttatcttcc
    22441 caggcgggaa ggtcaggaga ctgaatttgc tgacccagct gttgcagatg gcgtaacccc
    22501 actgaccccg ccgcaccttt aattttatgc ccttcctcaa caatgccttt tttatcctgc
    22561 gccgtcagat tcgactccag cacgctgaca tagcccggca tcattctctc aaacaccgct
    22621 aacccgtcgg tgatcagctt cggtcctaca agttcgagat actgttccag catgggaata
    22681 tcgagcaatg cttctgattt actgttctct tctgtcgtca ccgtactctc ctcatcatcc
    22741 tgggtatccc agaatttctt gatcatcgcg gttagcgccg gaacagaaag cggcttactc
    22801 agcacatcat ccattccagc attgaggtac tcttgcttgt ctttcagcac gttagcggtt
    22861 aaggccacca gcggcggtaa atcctcgcgc ggataacgct tcgtcagttc acgagagata
    22921 tccagcccgg tcatatctgg caactgaata tccagcaata ccaggtcgta ttcgcccggt
    22981 ttaaacatct ccagcgccgc cttgccggtc atggcgacat caacgctgtt acctaatttt
    23041 tccagcacag aacgcgcgac aatcacgttc agttcaatgt cttccaccag cagcacattc
    23101 agcgccggta aaggcatatc gtcttcatca aacgcatcat caacctcttc tgctaccgac
    23161 ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct gttcgctggt aaccgtaata
    23221 tcgccgccca tatttttcgc cagacgacga gaaacggcca gaccaatacc ggtgccggtg
    23281 gcaggtttac cgccatgact gtctttcacc tggtaataca tggcgaagat tttatccagc
    23341 tcatcctgcg gaatgccgat accagagtct tccacttcaa aatgcagcat atcgccttca
    23401 tcgtagcgca cgcgcacggt aacctggcct tgctgagtga atttgacggc gttactgatg
    23461 aggttccaca agatctgccg taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc
    23521 agcgtcggct ccagattaaa gcgcaatcct ttttgttgcg cctgcaatgc ggagagattt
    23581 tccagatcgg caaggaagct ggtgaaatca accggctgat tatcaagctg gaccttgcgc
    23641 cgttccatct tatccatgtc gataatatcg ttgaagatat tccccagcgt gacagccgaa
    23701 acatggatgg ttttgagata tttttcctgc tcggcggtga gttcagtatc cagcagaatg
    23761 cggctcaggc cgacgatacc gttcagcggt gtacgcaatt cgtgactgat ggtggagata
    23821 aacgtcgttt tgtcgcggct ggcgcgttca agcgcatcct gataccgctt acgctcggta
    23881 atgtcgcgac caaagcccat caaaccgtga cgtttaccca cgcggtcgta gtacggcact
    23941 ttacggattt caaagcaggc tttgcgtccg tcgggataat ccagccactg ctcataggtc
    24001 agtgacacat tatgacggaa cactttttca tcggtttcaa tgactttcgc agcggcttct
    24061 ggtgagtaaa catcagcagg tttcaggtga accagttgtt tttcgctttt tccggtcagc
    24121 agctccatcg cgcggttaca gccggaaaac tctttatctt cgttacgata aaaaaccagg
    24181 tcgggtgaag cgtcaaggaa ggaacgtaag aatgaggatt gctgctcgag ctgaatttgt
    24241 gtctcttcgc gctctttgat ttcaattttc agttggccga aggtttcctg cagttctgct
    24301 tccgcttttt cacgaacggc gatttcctga tttagctggg caatattatc ttttaactga
    24361 acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt tttgcaccag tcgtgacaga
    24421 cgttgtcgtg actcctccag ttgctcgacg accaccgata gaaagtagac cgcccacggc
    24481 gtaatcagca aaccaaagaa gatagaacga ataacatcaa tgctttcgac ctgaccatgc
    24541 agcaccatgg ttaccgccat ttgtaccaca atggcaagaa cgacgagggc cagcgccagc
    24601 aacattgaga agcgcaccag acctaacttc atcatcaggt caacataata ctgcgccagc
    24661 agacgaattt gcttcattag ggaattcctt cacgacaacc tgccccaata atacccaatt
    24721 ctgacagtta cgttataaat tgtgtgagaa atgcggaatt cccctcaatg gcctacgccg
    24781 ttggaggtag ccacggacgt cccagcgccg atccctgtac gccatgttca ttcagataac
    24841 gatccagctc gaccattccc gtccatcgat tttcgcacca cagcggagca agcagcgtcg
    24901 ggcgacgggc gctggcggaa atacggtggt agataacttc cggtggcgta tggcgaatca
    24961 tttctccggc agtgagcgtg taatcctcca gttcaattcc gttcaaacgc cccgcttccc
    25021 aggctttcgc cataatgctg cctttcacta tatgcagcgg atgcagcttt atgccgtcca
    25081 cgccggtttc aaccactcgc tccagcgttt gcaaacattc agcctgccct tcgccaggca
    25141 ggccaacaat taagtgagag cacaccttca gcccacgctc acgcgccagc tgcgtcgtgc
    25201 gctgatagca ggcaaaatca tgaccgcgat ttatgcgatg cagtgttttg tcgtgggcgg
    25261 tttgtagccc cagctccagc cacacttcgt agccctggtc cttatattcg caaagcagat
    25321 ccagcaccgc atccggcacg cagtccgggc gggtaccaac acacaaaccg acaatattgg
    25381 cctggctcac cgcctgctga tacatcgaac gcagcacctg aacttccgca aaggtgctgg
    25441 tatacgcctg aaagtaggcc agatagcgtt tagcgcggtt aactaaattc gcctggtgcg
    25501 ccagttgctc ggcaatggaa cgatgctgct gcgcttcatc ggcaaacgag gcaacattac
    25561 agaatgtgca gccgccacgc ccgatggtac cgtcacggtt agggcagcta aaaccgccgt
    25621 gcagcgtcag cttatgcacc ttttgcccat aacgacgggt gagatcacca ccaaacatat
    25681 tgactaattt ctgtaactgc ataatctgat agaccgcgcc ttgaaaagag gccaaagcct
    25741 gccattttta gcccaattcg gcgatgacct ggatcaatcg tcccagcctg cttttatcaa
    25801 ctgcataacc aatcaaaatt accgaaattt catgcacaat cacataaatc actgttgctt
    25861 atcttgtgtc agattttttt atcttcttat ggattttagg caaaaacagt agcatgaaac
    25921 gttattacca attaaggcag tataaaatgc tggctttctc atcaattcaa ggcaggataa
    25981 gggttaacac gcctttatga cagtcaggaa ttggctgttt ctctaacgac acccctttta
    26041 gccttaaata gaaaatccac tttaatttca tacaattaat aaagaatttt gcgctaaagc
    26101 acatttctgt accaataagc ttgccatttg acctgtatca gctttcccga taagttggaa
    26161 atccgctgga agctttctgg atgagcagcc tgctcatcat atttatgcag taattgagat
    26221 cccctcttca ccgtattaac cgatgcgaaa aggacaacaa gggggcgaat gcgaggtgcg
    26281 cgtatgacac gcaaaccccg tcgccacgct cttactgtgc ccgtgcgcag cggttcggaa
    26341 gtggggttcc cgcagagcct gggggaggtt cacgatatgt tgtacgataa atcccttgag
    26401 agggataact gtggtttcgg cctgatcgcc cacatagaag gcgaacctag ccacaaggta
    26461 gtgcgtactg caatacacgc actggcccgc atgcagcacc gtggcgcgat tctcgccgat
    26521 ggtaaaaccg gtgacggttg cggcttgctg ttacaaaaac cggatcgttt ttttcgcatc
    26581 gttgcgcagg agcgcggctg gcgtttagca aaaaactacg ccgtcgggat gctcttcctg
    26641 aataaagatc ctgaactcgc cgctgccgca cgccgcatcg ttgaagaaga gctgcaacgc
    26701 gaaacgttgt cgattgtggg ctggcgtgat gtccccacta acgaaggcgt gctgggtgaa
    26761 atcgccctct cctctctgcc acgcattgag caaatttttg tgaacgcccc ggcaggctgg
    26821 cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc gccgcattga aaagcgtctc
    26881 gaagccgaca aagacttcta cgtctgtagc ctgtcgaatc tggtcaatat ttataaaggt
    26941 ctgtgtatgc cggcggatct gccgcgcttc tacctggacc tcgcggacct gcgtctggaa
    27001 tcggccatct gcctgtttca ccagcgcttt tccactaaca ccgtaccgcg ctggccgctg
    27061 gcgcagccgt tccgctacct cgcgcataac ggtgaaatca acaccatcac tggtaaccgc
    27121 caatgggcgc gtgcgcgtac ctataaattc cagacgccgc tgatccctga cctgcacgac
    27181 gccgcgccgt tcgtcaacga aaccggctct gactccagtt cgatggataa catgctggaa
    27241 ctcctgctgg caggtgggat ggatatcatc cgtgccatgc gtctgttagt gccacctgcc
    27301 tggcagaaca acccggatat ggacccggaa ctgcgtgcct tctttgactt taactccatg
    27361 catatggagc cgtgggatgg cccggcaggc atcgtgatgt ctgacggtcg ttttgccgcc
    27421 tgtaacctcg accgtaacgg tctgcgtccg gcgcgctacg tcatcaccaa agataagctc
    27481 attacctgcg cctctgaagt cggtatctgg gattatcagc ctgacgaagt ggtcgaaaaa
    27541 ggccgcgtgg ggccaggcga actgatggtt atcgacaccc gcagtgggcg tattctgcac
    27601 tcggcagaaa ccgatgacga tctgaaaagc cgccaccctt ataaagagtg gatggagaaa
    27661 aacgtccgcc gtctggtacc gtttgaagat ctgcccgatg aagaagtggg tagccgcgaa
    27721 ctggacgacg acacgcttgc cagctaccag aaacaattta actacagcgc ggaagagctg
    27781 gattcggtga tccgcgtact gggtgaaaac ggtcaggaag cggtcggttc gatgggcgac
    27841 gataccccat tcgccgtgct ctccagccag ccgcgcatta tttacgacta cttccgccag
    27901 cagtttgctc aggtaaccaa cccgccaatc gacccgctgc gcgaagcgca tgttatgtcg
    27961 ctcgccacca gtatcggtcg tgaaatgaac gtcttctgtg aagcagaagg ccaggcgcac
    28021 cgtttaagct ttaaatcgcc gattctgctc tattccgatt tcaaacagct cacgacgatg
    28081 aaagaggatc actaccgcgc agatacgctg gatatcacct ttgacgtcac taaaaccacg
    28141 ctcgaagcga cggtgaaaga actgtgtgac aaagccgaaa aaatggtacg tagcggcacc
    28201 gtgctgctgg tgctctccga ccggaatatc gctaaagatc gcctgccggt tccggctccg
    28261 atggcggttg gcgcgatcca gacccgtctg gtcgagcaaa gcctgcgttg cgatgccaac
    28321 atcatcgtag aaaccgccag cgcccgtgat ccgcaccact tcgccgtgtt gctgggtttc
    28381 ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc tgggccgcct ggtagacacc
    28441 cacgcgattg ccaaagatta tcgtaccgtg atgctcaact atcgtaacgg catcaacaaa
    28501 ggcttgtaca aaatcatgtc caaaatgggc atctccacca tcgcctctta ccgctgctcg
    28561 aaactgtttg aagcggtcgg tctgcacgat gatgtcgtgg gcctgtgctt ccagggggca
    28621 gttagccgca ttgggggggc gagctttgaa gacttccagc aggatctgct gaatctgtcg
    28681 aaacgagcct ggctggcgcg taagcctatc agccagggcg gcctgctgaa atatgtacac
    28741 ggtggcgaat accacgccta caacccggac gtggtgcgca cgctgcaaca ggcggtacag
    28801 agcggcgagt atagcgacta tcaggaatac gcgaagctgg ttaatgagcg tccggcaacc
    28861 acgctacgcg atctgctggc aattacgccg ggtgaaaacg cggtcaacat tgctgatgtt
    28921 gaaccggcaa gcgaactgtt taaacgcttc gataccgccg cgatgtctat cggtgcgtta
    28981 agcccggaag cccacgaggc gctggcggaa gcgatgaaca gcatcggcgg taactcaaac
    29041 tccggcgaag gcggtgaaga cccggcgcgc tatggcacta acaaagtgtc gcgcatcaag
    29101 caggtagcgt ccggtcgctt tggggttact ccggcgtatc tggtcaatgc cgacgtcatt
    29161 cagataaaag tcgcccaggg cgcgaagccg ggtgaaggtg gtcagttacc gggtgataaa
    29221 gtcactcctt acattgccaa actgcgctat tcggtgccgg gcgtgacgct gatctccccg
    29281 ccgccgcacc acgatatcta ctctattgag gatttagcgc agctgatttt cgacctcaag
    29341 caggtcaatc caaaagcgat gatctccgtg aagctggttt ccgaaccggg cgtaggcacc
    29401 atcgcgactg gcgtggcaaa agcttatgcc gatttaatca ccatcgcagg ctatgacggc
    29461 ggcaccggcg caagtccgct ttcatcggtg aaatacgcag gctgtccgtg ggagctgggg
    29521 cttgttgaaa cccaacaggc gctggttgcc aacggcttgc gtcacaagat ccgtttgcag
    29581 gtcgatggcg gcctgaaaac gggtgtcgat atcatcaaag cggcgattct tggcgcggaa
    29641 agttttggct tcggcactgg cccgatggta gcgcttggct gtaaatatct gcgaatttgc
    29701 cacctgaaca actgcgccac gggtgtagca actcaggatg acaaactgcg taagaatcac
    29761 tatcacggcc tgccgttcaa agtgactaat tactttgagt ttatcgcccg tgaaacccgc
    29821 gagctgatgg cacagctggg ggtaacgcgt ctggtggatc tgattggtcg caccgacctg
    29881 ctgaaagagc tggacggttt caccgccaaa cagcagaaac tggcactgtc gaagctgctg
    29941 gagactgccg agccgcatcc tggtaaagcg ctctactgca ccgaaaacaa cccgccgttt
    30001 gataacggcc tgctgaacgc gcagttgctg caacaggcaa aaccgtttgt cgatgaacgc
    30061 cagagcaaaa ccttctggtt cgatatccgc aacaccgacc gttctgtcgg cgcgtcgctt
    30121 tcaggctata tcgcccagac gcacggcgat caggggctgg cagccgatcc tatcaaagcg
    30181 tacttcaacg gcactgcagg ccagagcttc ggcgtgtgga acgcgggcgg cgtggaactg
    30241 tacctgaccg gtgatgccaa cgactatgtt ggtaaaggca tggcgggcgg cttaatcgcc
    30301 attcgtcctc cggttggttc cgccttccgc agccatgaag cgagcattat cggcaacacc
    30361 tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag gccgcgcggg tgaacgtttc
    30421 ggcgtgcgta actccggtgc tatcaccgtg gtagaaggca ttggcgacaa cggctgtgaa
    30481 tatatgacgg gtggtatcgt ctgtattctg ggtaaaaccg gcgttaactt cggcgcgggc
    30541 atgactggcg gcttcgctta cgttctcgat gaaagcggcg atttccgcaa acgcgttaac
    30601 ccggagctgg tcgaggtctt aagcgttgac gatctggcga tccatgaaga gcatctgcgt
    30661 ggtcttatca ccgagcatgt tcagcatacc ggttcccagc gtggtgaaga gattctggcg
    30721 aactggtcaa ccttcgccac taaatttgcg ctggttaaac cgaagtccag tgatgtaaaa
    30781 gcactgctgg gtcaccgtag tcgtagcgca gcagagttgc gcgtgcaggc gcagtaaggg
    30841 gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc tgcagcgcgt tgatccgcca
    30901 aagaaaccgc tgaagatccg caaaattgag tttgttgaaa tttacgagcc gttttccgaa
    30961 ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg gcaacccata ctgcgagtgg
    31021 aaatgcccgg tacacaacta catcccaaac tggctgaagc tcgccaatga ggggcgtatt
    31081 tttgaagcgg cggaactgtc gcaccagaca aacaccctgc cggaagtttg cggacgtgtc
    31141 tgcccgcaag accgtctgtg cgaaggttcc tgcaccctga acgatgagtt cggtgcggtg
    31201 accatcggca acattgagcg ttatatcaat gataaagcgt tcgagatggg ctggcgtccg
    31261 gatatgtccg gcgtgaaaca gaccggtaaa aaagtggcga ttatcggcgc aggtccggcg
    31321 ggtctggcgt gcgcggatgt cctgacgcgc aacggcgtaa aagcgatcgt cttcgaccgt
    31381 cacccagaaa ttggcggctt gctgaccttc ggtattccgg ctttcaagct ggaaaaagag
    31441 gtaatgacgc gccgccgtga aatcttcacc ggcatgggta ttgaattcaa actcaatacc
    31501 gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg attacgatgc cgtgttcctt
    31561 ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa acgaagacgc cgatggcgtg
    31621 tacgcagcgc tgccgttcct tatcgccaac accaaacagt taatgggctt tggtgaaacc
    31681 cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg tggtccttgg cggtggcgac
    31741 actgcgatgg actgcgtgcg cacgtctgta cgccaggggg caaagcacgt tacctgtgcc
    31801 tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg aagtgaaaaa cgcgcgggaa
    31861 gaaggtgttg agttcaaatt caacgtccag ccgctgggta tagaagtgaa tggcaacggc
    31921 aaagtcagcg gcgtaaaaat ggtgcgcaca gaaatgggcg aaccggatgc caaaggccgt
    31981 cgccgcgcgg agattgtggc aggttccgaa catatcgttc cggcagatgc ggtgatcatg
    32041 gcgtttggtt tccgtccaca caacatggaa tggctggcaa aacacagcgt cgagctggat
    32101 tcgcaggggc gcatcatcgc cccggaaggc agcgacaacg ctttccagac cagcaacccg
    32161 aaaatctttg ctggcggcga tatcgtccgt ggttccgatc tggtcgttac cgccatcgcc
    32221 gaaggtcgca aagcggcaga cggcattatg aactggctgg aagtttaagc gaggtaacaa
    32281 tgaattcgcc agacagtgtc tggcgaattt ataaaacgag ctatccaact aaattttaag
    32341 ccattaaatc agccttaacc ttcactacca cctttttaat ctcaccaggt tcgccaacaa
    32401 cgcatcccgg tttatgcggt tctcctggca taaacacggc gaacattccc ggtttcagga
    32461 tgatggcttg ctcgttttca atggcgctgc aaagctgata atcatcttca tggtggaact
    32521 cttcacactg acgcgcagtg cctgccatgc caaacagaat ccgttcctca ccgtttaata
    32581 acagctggat atcaatgtat tgttcgtgca attcggcttt tttctcaacg ggcgattgag
    32641 tattaaatgt catgacattc ataaagatat tgtcgccctg taattcgtaa cggccaggcg
    32701 ctttttcttg cggtctggca gctaatgcca acgttaacgc gtcctgtaac gcaggatgta
    32761 atccggcaga cggtaatgac tgtacttcac ccatcatcat aatatttctc cctgggccaa
    32821 cagcgcagcc ccaagtaaac ctgcatcgtg gcggtagtgc gccgccagta aatcaacatg
    32881 aaatgccgct ggctcctgcg ccagatacgt ttccaccagc gccagatacc cttctgccag
    32941 accaacgctg ccaccgacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat
    33001 cagccttgca agcgtacgtg cggagcggtg aatcagttgc tgcgcctgct catcgccctg
    33061 cccggcgcgc gtgaaaatag ttttcgcatt cgcaccagcc aactccccct gcgctgcagc
    33121 tgcaatgccg cgaccagaag caatcgcttc cacgcaacct gtgcgtccac agccgcagac
    33181 tggaccatgc ggatcggcaa gcgtatgccc gatatgcccc gccagaccgc cagggccggt
    33241 acgcagttta ccgccgctca ctacaccgcc gccaacgccg gtggaaacag tgataaacac
    33301 catatcggtt atatcgccat ccagcgcctg atactccgcc catgctgcgg cctgcgcatc
    33361 attaatggca atggtcggca aattggtaag ttgctccagc gttttgacta acggaaagtg
    33421 taacaatcca ccgagattat gcggattcag cgccagcaag ctgccgtcgc gaataattcc
    33481 ggttgaagcg atagcaaccc gctgcgcatg tgcttgcaac ggagagacta atgcggataa
    33541 ggcatcacgc agcgcctgcg gagtctggct ggcaggcgta ggaagttctc ggcgatcgcg
    33601 gatctgcccg tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc
    33661 cagtgtggtc atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca
    33721 agacgcgtaa tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatccgcc
    33781 gcctgagcag gcgtgttgta acgcccttcg gcaatcaccc ggcatccggc gtcactcaac
    33841 gttttcacca gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc
    33901 gtagtgccaa taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag
    33961 cagtcggtca tcgccagtaa accgtgatgg tgaatacgtg ccagcagcgt ttcaacaggc
    34021 accggacgcg gtcggtcagt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc
    34081 gcatcaacat cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgcttc
    34141 acaatcccaa taatcggcac gctcaccacc gcacgcgtgg cttgcagatt tgccacacct
    34201 tcaatacgaa tggcaaccgc gcccgcctgc tctgccgcta atgccatggc agcgacgatt
    34261 tcgggtttat cgagcgggct gtccggaacc ggctggcagg agacaatcag gccaccgtta
    34321 gcagcgattt tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc
    34381 ccggtacaga gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc
    34441 tgccaaatgg cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg
    34501 cttccgggcg caaccaacgc tgaacacaag aaggcatatc cagaccaatc agcaggatca
    34561 ccacaaacgt taaactaaac gagagcgagc caagtgccgt tcccagatcc agacgttgag
    34621 cgatcaacgc gccgaggatt ggagctagtg caccgcccag tgcgccaacg ttgtaggtaa
    34681 agcccagccc cgccgcacgc tggtcggtat cgaaataacc gccgatcagt ttcggtaaaa
    34741 tccctgagat gccctgacca agcatttgct ggaagaacag taacaaaccg agcacccaga
    34801 cgtttgcgcc gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcagactac
    34861 aaacgtacgc tttgcgggtt cccagccagt caccgaggaa gccaccgacg cagcacccca
    34921 ccgccgcgcc aaagccacta aagaacagca cattggcgac agtatgcggg tcataagcca
    34981 gatcggtttt cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcga
    35041 acaacacgac caccatcagc attacgcccg ttggccagcg tttgcccgta ctctgcacca
    35101 taaagctgat aaagattgcg gcgcataaca gcccgagtac agcgacgatt gcggcatttt
    35161 gcaggttacc ggcgaagcag aaccacagcg cagtagccgc cgccagcgtc attacgatat
    35221 tggcaatgcg atgttcacca cggtaaagaa tgtctaccat cgtgcgtact ggtgctttac
    35281 ccccgtgttt ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa
    35341 agatgattgg caaaatgccg ataaagaaca gcgcacgcca gccccagacc ggaaccacca
    35401 gactatagac ctgagcggca acgacggccc ccactgagaa gcctgaaatc agaaaaccac
    35461 tggctttgtt acgcagatgc tttggccagc tttcaatgac ataggtggcg ctggaaccgt
    35521 attcacccgc catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg
    35581 gcgcaaagcc gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca
    35641 gacgacgccc gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga
    35701 taaaggctgc ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta
    35761 cttcggtgag taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc
    35821 aggcagcgga aaatgcccgc cattgtgcgc ggttaagatg gcgataccac gggatattct
    35881 gggttgtagt actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga
    35941 aaattgtagg gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa
    36001 caactcaccc gcgctcttgc atcaactgct gagccagcgc cttcagttct ggcagatatt
    36061 tttcatctac cggaccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat
    36121 gcaggacagt tttcagccca cggaatacgc cagttttgat cagtaaatca atgactttgt
    36181 tgcactcagt ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga
    36241 tcccctgata acgccagccc atgatgttgt aagtgctgcc gataccgccg tctgcgcccg
    36301 ccagaagacc agaggcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt
    36361 cacgacggat ctgctccatc tgatagagat cgccagaggt ctgtttcagc gcacctacgc
    36421 caggcagcgt aaccaaggtg ttgatctgat ccagggtcag ttttaccccg ctcagggctg
    36481 gaatgttgta caccaccatc ggcaacccat ccgctgaatc aataattgcc cgatagtgat
    36541 cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga
    36601 agccataacg tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga
    36661 catgggcgat gagtttaatc ttacctttcg cctcttcagc gacgatttcc agtacctgtt
    36721 cgcgctcgga aaggctttgt acaaaggctt cgccggtcga accacccacg tacaaaccgt
    36781 cgatgccctg ctgaatattg aactgaacca ggcggcgcag actcgcttta tccagtgctt
    36841 gttgttggtc aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa
    36901 atacctctga agtgatgctt gtctgataaa cgatatacct ttatacctgt tataccagat
    36961 caattaagca acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat
    37021 tatttctttt tgttggtggt ctgaccgaag gcgtgccagg tagcagagac gctgttgaga
    37081 tgcgattgca acgcgcgatc ggcttcgtca ggatcatgac ggcggatcgc gtcaacgatc
    37141 gcaatatgct gttgatagct aacgttgtta tgctcatgca gagcctgatc ggtcacggtt
    37201 ggacgtgcgg cgattagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta
    37261 cccgggatct ccgccagaac gcggtggaaa tcaacgtctg agcgaatgaa tgccgcgttg
    37321 ttatccagcg actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg
    37381 gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg
    37441 aaatgggcaa tcccaccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg
    37501 atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt cgccgttgtt tatttgcacc
    37561 agacctttgc gttttaacgc tgccagcgct tcgcgcaccg aaggacgccc gacgttaaag
    37621 aacgccatca gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg
    37681 atcatctgtt ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg
    37741 ctgcgcaagt tgcgcccgat agtggtagta gaatcttcag cttgcggatc aaatgcgttc
    37801 ataggactca ttcaggaaaa tgcgttagcg gataataacg acgctatcgt agcacagtat
    37861 caaagtcagg cgaaaagatg acagatagca aaacaggcca catgggcctg ttttcatttt
    37921 acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag
    37981 gatgagtttc atcatcgtga tctggcttgt cgccatcgat aaccgacata acggtttcgt
    38041 tgtctgccga tgcctcttca tcattcatga tgctggcatc ttcatcgtag gcagcttcag
    38101 gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg
    38161 gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg
    38221 ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg
    38281 catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga
    38341 gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgtta
    38401 gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt ctgcttcagt
    38461 taaagaagca aggaaagagt cacgttcaaa gacgcgggtc atatagcctt tcagctcttt
    38521 cgcacccggg ccgctgaact cgatgcccag ttgcggcaga cgccacagca gaggagcaag
    38581 gtagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg
    38641 cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatcggctt cagaagctga
    38701 accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag
    38761 acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc
    38821 atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt
    38881 cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac
    38941 gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata
    39001 gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat
    39061 gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga
    39121 agttgtgtta cccagtaagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca
    39181 atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg acctttgtgg
    39241 tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag
    39301 tcccttattg gcgatgtggt ttttgtttta cctgtctgtc aggtggcagc aaaaagcaac
    39361 tttccatttt ttacgctgat gcagatttta gccataaaaa aacccgccga agcgggtttt
    39421 ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac
    39481 gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga
    39541 agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga
    39601 taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca
    39661 ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc atgcgagcag
    39721 tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg
    39781 gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc agtgccgtag tattgatttt
    39841 cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt gccgcgtggt
    39901 tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt
    39961 ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct
    40021 cttcaaaggt cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt
    40081 cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac
    40141 cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt
    40201 cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta
    40261 cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa tagttacacg
    40321 ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct
    40381 accccttcga atagcctatg ccagcacaca aaaagtttcg ggaaaaaaaa ctttcttgta
    40441 acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt
    40501 gtggcaggtt ttaccccgcc aaatgctcgc gcttcaaata ctcttcgctt tgcatctctt
    40561 gcagacgtga caggcaacgc tggaactcaa acttcagccg ctcgccctga taaatttcat
    40621 acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca
    40681 gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt acatcaaaca
    40741 acatgaccgt gtgaaagagg cgtgagagcg caatatagtc atgctgactg cgggcgtcga
    40801 cgcacagcgt agtaaaagaa accgccagcg tctggttctc gacgcccatt gtcgccaatg
    40861 gccgatggtt gatttctaac gtcggtgaat tttctcgttt cgcccccgcc agcgccaacc
    40921 atagtttatc catttgcgcc cgggtttcat cgttgagggg cgaaagccac agatgcgcct
    40981 gagtgagtgt acgcaggcga taatcaacac cagcgtccac gttcattaca tcacaatgct
    41041 gtttaatggc atcgattgca ggcaaaaaac gcgcacgttg caggccattt cgataaagtt
    41101 cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac agggctttca
    41161 tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg tcaaaacaga
    41221 gcacgtcagt ttctgcttta aagcgatcgg caataatttc cagcggatcg gtctgcccct
    41281 gcaaagcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc aggcgctgtt
    41341 tccgctctcc cggcagactt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta
    41401 caccgcccca catatataag ccacgcactg gcatatgctt tgtgtcttcg cgtttacccc
    41461 acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc gtgctattga
    41521 cgagttcctt ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt
    41581 gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga
    41641 tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc
    41701 cgttctacac tacgcgatat gctgtcggga ttccacttct gtggaattaa cggttatagt
    41761 ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac aaaagacaac
    41821 gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg
    41881 gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg
    41941 aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg
    42001 cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca
    42061 tggcgaaaag ctccagtagc ctgctgccgg aactgtctgc ggaagcaaac ccgttccgta
    42121 atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact
    42181 attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttg
    42241 gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc
    42301 tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc
    42361 attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt gcattagcgt
    42421 taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg
    42481 ttgccgatca ggcccccctc cccagtctgg ctccaatgct ggaaaaagtg cttccggcag
    42541 tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca
    42601 aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa ggtttaggct
    42661 ccggtgtcat catcaacgcc aataaaggct atgtgctgac caacaaccat gtgattaatc
    42721 aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg
    42781 gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc aaattaacgc
    42841 aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg gcggtcggta
    42901 acccatttgg ccttgggcaa accgctacct ccgggattat atctgcgtta ggccgcagcg
    42961 gtctgaatct cgaaggtctg gaaaacttta ttcaaactga tgcctccatt aaccgcggca
    43021 attccggtgg tgcgctgtta aaccttaacg gtgagttaat tggcatcaac actgcaatcc
    43081 ttgcgcctgg cggcggtagc gtcgggattg gatttgccat tcccagtaat atggcgcgaa
    43141 cactggcgca gcagcttatc gactttggag aaatcaaacg cggcctgtta ggtatcaaag
    43201 gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt
    43261 ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa gcgggcgata
    43321 ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg
    43381 cgaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc aaaccactgg
    43441 aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa atgatcacgc
    43501 cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggtggc aaaggtatta
    43561 agatcgatga ggttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg
    43621 tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg
    43681 cggcaaaacc ggccataatc gccttgcaaa tcatacgcgg caatgaaagc ctctacctac
    43741 taatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg ttaactcgtg
    43801 gtatgctgct gccgttccct tttttaacga tgcctccatc atgtttgtga agctcttacg
    43861 ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg
    43921 cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgaaacgc ctgccagcta
    43981 taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttataacc gcggtttgaa
    44041 caccaactct cacaaccagc ttgagatccg caccctggga tctggtgtaa tcatggatca
    44101 acgcgggtat atcatcacca ataaacacgt gatcaacgac gccgatcaga tcatcgtcgc
    44161 cttacaggat ggacgtgttt ttgaagcgtt actggttggc tctgactctc taaccgatct
    44221 ggcggtgctg aaaattaacg ccacaggcgg tttaccaacg attccgatca atccacgccg
    44281 cgtaccgcac attggcgatg tggttctggc gatcggtaac ccgtacaacc tcgggcagac
    44341 cattacccag gggattatca gtgccacggg tcgaatcggt ctgaacccga ccgggcggca
    44401 aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa
    44461 ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga
    44521 aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcgaccaaaa ttatggataa
    44581 actgatccgt gatggccgtg tgatccgtgg ctacattggc attggtggac gtgagatcgc
    44641 cccattacat gcgcagagtg gcggcatgga tcaactgcaa gggatcgtgg ttaacgaagt
    44701 gtcgcctgat ggcccagcgg caaatgcagg catccaggtc aacgatctga ttatctctgt
    44761 gaataacaaa cccgctattt ccgccctgga gacaatggat caggtcgcgg aaattcgccc
    44821 tggttcggtg atcccggttg tcgtgatgcg tgacgataaa cagttaacat tgcaggtcac
    44881 cattcaggaa tatccggcga ccaactaagg tcagcgtaaa aaaacaaaaa ccggagtctg
    44941 tgctccggtt ttttattatc cgctaatcaa ttacttatta acgaactctt cgcccagggc
    45001 gatatctttc ttcagcgtat ccagcatacc ttccagcgcg ttctgttcaa atgcgctcag
    45061 agtaccgata gatttacgct cttccacgcc gtttttaccc agcagcagcg gttgagagaa
    45121 gaaacgagcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc
    45181 ctgcagtgcg cgaaccagag acagaccaaa acgtgcagct gcctggccca tagacagggt
    45241 tgcagacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt
    45301 ggtcagatca gccacttcct gctcggtaaa actaacgcca ggaacctgtg acagcagcgg
    45361 cagaatggta acaccagagt gaccaccaat aaccggcact tcaacttcgc ctggctgttt
    45421 gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa
    45481 cagtttgttt ttgtcataaa caccggcttt tttcagcact tcagcagcaa tcgcaactgt
    45541 agtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg
    45601 ctgtaccagg tttttcacga taccggcgtt aacgttaaac aggtcggaac gatccatacc
    45661 cggtttacgc gctacgcctg cagagataag aacgacatct gcaccttcca gcgccggagt
    45721 cgcatcttca cctgaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac
    45781 agccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag
    45841 ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc
    45901 gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc
    45961 gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc
    46021 aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg taattaacag
    46081 tcacttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta ccgctcagcc
    46141 aaaacaatat caatttgata acaattaatt tacttttaag cagaatttgc aggccgtgac
    46201 gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc
    46261 agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa taatgttgta
    46321 tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag
    46381 catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt
    46441 tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt
    46501 ttggtgcggt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac
    46561 tgggtgtacc aaccacctcc agtccattga agaatctggt actggatatc gactacaacg
    46621 atgcagttgt cgtgattcat accagccctg gtgcggcgca gttaattgct cgcctgctgg
    46681 actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta
    46741 ctacccctgc taacggtttc accgtcaaag agctgtacga agcgatttta gagctgttcg
    46801 accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttttgcc
    46861 tatcaaccca ctgctttccc ctgcgattca tttaacgaat agtgcgtttt actgcgacat
    46921 atcattcaca caatgaatac atatagtaaa aaagcacatt atgcaaaatt cattatctaa
    46981 ttgaaaatac tataattaac aataaatgat cgtgttttta attctttttt gttattaaaa
    47041 ttcacatttt taacacttag tatcaactga aacagttagc gtggtattaa ttagttcaat
    47101 aattagtgta tacttgattt tgtgatatgg gtcacgaaac aaaggcccag ctaaaagatt
    47161 atgtcgaggt aaaaatcatg aaaatcaaaa ccactgttgc tgcattaagc gtactttctg
    47221 ttctctcttt cggtgcattc gctgccgact ccattgatgc tgcacaagca caaaatcgtg
    47281 aagcaatcgg gaccgtatcc gtaagtggtg tggcgtcttc gccaatggat atgcgtgaaa
    47341 tgctgaacaa aaaagcggaa gagaaaggcg caacggccta ccagattact gaagctcgta
    47401 gtggtgacac ctggcacgct acggctgaac tgtacaaata aaccctcatc gtcttgtccg
    47461 acgatattgc ccccggttcg ggggcttttt ttgcgctaat gacgaacatt aaaacgcaaa
    47521 tgcccttcca gctcttcctc tgcctcatca aacagcaata ttaacgcgcc aaaacggcga
    47581 cgcgttttct ctcccagatg aacaaattca atctcaagtg gtagcggcag gacatcgttc
    47641 attaacacat cccatagtga gtcgagatcg cgtaccttat ctttcgccag accaaaggtt
    47701 tggctaaagt cacgataaaa atcctcctga ctctcaatct catcaaaatc aaaggtataa
    47761 atattcatct gttgccaccg tcacgtttcg cggcgagttc tgccgttcat cgctaagta
//
LOCUS       DAOFSV010000021        47490 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.021, whole genome shotgun sequence.
ACCESSION   DAOFSV010000021 DAOFSV010000000
VERSION     DAOFSV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.021"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            191..634
                     /gene="trg"
                     /locus_tag="QBH27_001294"
                     /pseudo
     CDS             191..634
                     /gene="trg"
                     /locus_tag="QBH27_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(672..1595)
                     /locus_tag="QBH27_001295"
     CDS             complement(672..1595)
                     /locus_tag="QBH27_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7013630.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            1812..3155
                     /locus_tag="QBH27_001296"
     CDS             1812..3155
                     /locus_tag="QBH27_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ7013631.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDAASLARNPFRVFTSLLRLELIENELLRQKAAEILRQRDIFTPRCR
                     QLLQEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEKTYRALHKEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            3380..5035
                     /gene="opgD"
                     /locus_tag="QBH27_001297"
                     /gene_synonym="mdoD"
     CDS             3380..5035
                     /gene="opgD"
                     /locus_tag="QBH27_001297"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDQ7013632.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            5175..5399
                     /locus_tag="QBH27_001298"
     CDS             5175..5399
                     /locus_tag="QBH27_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDQ7013633.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            5462..6001
                     /gene="rimL"
                     /locus_tag="QBH27_001299"
     CDS             5462..6001
                     /gene="rimL"
                     /locus_tag="QBH27_001299"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDQ7013634.1"
                     /translation="MTETIKVSESLELHAVAESHVTLLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     PHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(5993..6973)
                     /locus_tag="QBH27_001300"
     CDS             complement(5993..6973)
                     /locus_tag="QBH27_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDQ7013635.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            7097..8089
                     /gene="tehA"
                     /locus_tag="QBH27_001301"
     CDS             7097..8089
                     /gene="tehA"
                     /locus_tag="QBH27_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDQ7013636.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLCLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            8086..8679
                     /gene="tehB"
                     /locus_tag="QBH27_001302"
     CDS             8086..8679
                     /gene="tehB"
                     /locus_tag="QBH27_001302"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDQ7013637.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCAKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            8982..9650
                     /locus_tag="QBH27_001303"
     CDS             8982..9650
                     /locus_tag="QBH27_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDQ7013638.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(9685..10896)
                     /gene="ydcO"
                     /locus_tag="QBH27_001304"
     CDS             complement(9685..10896)
                     /gene="ydcO"
                     /locus_tag="QBH27_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDQ7013639.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQELT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLAAKAVAPRYAVIAAMIIGIVIVTAQGDVVTTDIVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            10949..11485
                     /gene="sutR"
                     /locus_tag="QBH27_001305"
     CDS             10949..11485
                     /gene="sutR"
                     /locus_tag="QBH27_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDQ7013640.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESMPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11558..13519
                     /gene="rlhA"
                     /locus_tag="QBH27_001306"
     CDS             11558..13519
                     /gene="rlhA"
                     /locus_tag="QBH27_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDQ7013641.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATNATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWHQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKAMNNLKDGLTKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(13611..13841)
                     /locus_tag="QBH27_001307"
     CDS             complement(13611..13841)
                     /locus_tag="QBH27_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HDQ7013642.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            14020..14256
                     /gene="hicB"
                     /locus_tag="QBH27_001308"
                     /pseudo
     CDS             14020..14256
                     /gene="hicB"
                     /locus_tag="QBH27_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            14335..15741
                     /locus_tag="QBH27_001309"
     CDS             14335..15741
                     /locus_tag="QBH27_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDQ7013643.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            15986..17131
                     /locus_tag="QBH27_001310"
     CDS             15986..17131
                     /locus_tag="QBH27_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ7013644.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPVAEGGKFVPYSRWTQDYIAIMGGR"
     gene            17149..18162
                     /locus_tag="QBH27_001311"
     CDS             17149..18162
                     /locus_tag="QBH27_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013645.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAEKLCGMTGSFALRPEHIRLNTHGEMQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            18163..19104
                     /locus_tag="QBH27_001312"
     CDS             18163..19104
                     /locus_tag="QBH27_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013646.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            19094..19888
                     /locus_tag="QBH27_001313"
     CDS             19094..19888
                     /locus_tag="QBH27_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013647.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            19910..21334
                     /gene="patD"
                     /locus_tag="QBH27_001314"
     CDS             19910..21334
                     /gene="patD"
                     /locus_tag="QBH27_001314"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDQ7013648.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDSE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(21431..21526)
                     /gene="yncL"
                     /locus_tag="QBH27_001315"
     CDS             complement(21431..21526)
                     /gene="yncL"
                     /locus_tag="QBH27_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDQ7013649.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            21722..21895
                     /gene="ortT"
                     /locus_tag="QBH27_001316"
     CDS             21722..21895
                     /gene="ortT"
                     /locus_tag="QBH27_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDQ7013650.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            21981..22214
                     /locus_tag="QBH27_001317"
     CDS             21981..22214
                     /locus_tag="QBH27_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDQ7013651.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTL
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(22215..22664)
                     /locus_tag="QBH27_001318"
     CDS             complement(22215..22664)
                     /locus_tag="QBH27_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ7013652.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(22661..23179)
                     /gene="mddA"
                     /locus_tag="QBH27_001319"
     CDS             complement(22661..23179)
                     /gene="mddA"
                     /locus_tag="QBH27_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDQ7013653.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            23428..23583
                     /locus_tag="QBH27_001320"
                     /pseudo
     CDS             23428..23583
                     /locus_tag="QBH27_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(23660..25762)
                     /locus_tag="QBH27_001321"
     CDS             complement(23660..25762)
                     /locus_tag="QBH27_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ7013654.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVATTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDMRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            26004..27065
                     /locus_tag="QBH27_001322"
     CDS             26004..27065
                     /locus_tag="QBH27_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDQ7013655.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWIATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDTTNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTTDNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(27178..28677)
                     /gene="ansP"
                     /locus_tag="QBH27_001323"
     CDS             complement(27178..28677)
                     /gene="ansP"
                     /locus_tag="QBH27_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDQ7013656.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQDIVFKPETAS"
     gene            29489..30625
                     /locus_tag="QBH27_001324"
     CDS             29489..30625
                     /locus_tag="QBH27_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDQ7013657.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFDNGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITPIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            complement(30696..31265)
                     /locus_tag="QBH27_001325"
     CDS             complement(30696..31265)
                     /locus_tag="QBH27_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDQ7013658.1"
                     /translation="MSQFIPVELHHASCLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEGNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGMFVTSGKRVTAG"
     gene            31440..32285
                     /gene="nhoA"
                     /locus_tag="QBH27_001326"
     CDS             31440..32285
                     /gene="nhoA"
                     /locus_tag="QBH27_001326"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDQ7013659.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVIGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            complement(32381..33274)
                     /locus_tag="QBH27_001327"
     CDS             complement(32381..33274)
                     /locus_tag="QBH27_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDQ7013660.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(33354..34034)
                     /gene="narI"
                     /locus_tag="QBH27_001328"
     CDS             complement(33354..34034)
                     /gene="narI"
                     /locus_tag="QBH27_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDQ7013661.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(34031..34726)
                     /gene="narW"
                     /locus_tag="QBH27_001329"
     CDS             complement(34031..34726)
                     /gene="narW"
                     /locus_tag="QBH27_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDQ7013662.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILTLLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(34726..36270)
                     /gene="narH"
                     /locus_tag="QBH27_001330"
     CDS             complement(34726..36270)
                     /gene="narH"
                     /locus_tag="QBH27_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDQ7013663.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(36267..40007)
                     /gene="narZ"
                     /locus_tag="QBH27_001331"
     CDS             complement(36267..40007)
                     /gene="narZ"
                     /locus_tag="QBH27_001331"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDQ7013664.1"
                     /translation="MSKLLDRFRYFKQKGEIFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFAFDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            complement(40102..41490)
                     /gene="narU"
                     /locus_tag="QBH27_001332"
     CDS             complement(40102..41490)
                     /gene="narU"
                     /locus_tag="QBH27_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDQ7013665.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(41763..42644)
                     /gene="yddG"
                     /locus_tag="QBH27_001333"
     CDS             complement(41763..42644)
                     /gene="yddG"
                     /locus_tag="QBH27_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDQ7013666.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAVYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            42876..45923
                     /gene="fdnG"
                     /locus_tag="QBH27_001334"
     CDS             42876..45923
                     /gene="fdnG"
                     /locus_tag="QBH27_001334"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:43461..43463,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDQ7013667.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            45936..46820
                     /gene="fdnH"
                     /locus_tag="QBH27_001335"
     CDS             45936..46820
                     /gene="fdnH"
                     /locus_tag="QBH27_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDQ7013668.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDEEEGNHE"
     gene            46813..47466
                     /gene="fdnI"
                     /locus_tag="QBH27_001336"
     CDS             46813..47466
                     /gene="fdnI"
                     /locus_tag="QBH27_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDQ7013669.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
BASE COUNT        11994 a        11760 c        12253 g        11483 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc aggttagcct gaaaacagaa
       61 agtctcaggc acccgtgagc atcctatgtg gtttccttag ggacgaaaat aatcacctga
      121 cgaaatcgcg tgctgctttc cagaattttt cgtcattcgg tttagccagt ttaatcattc
      181 gttactctcg caaggccgtg gatttgccgt tgtcgccagc gaagtacgga cactcgcaag
      241 ccgcagcgcc caggcggcga aagagattga aggcttgatc agtgaatcag tcaggttaat
      301 tgacctggga tcggatgagg tggcaacggc agggaaaacc atgtgcacta ttgttgatgc
      361 cgtcgcgagt gtcacacata tcatgcagga aatcgccacc gcctcgggtg aacaaagtcg
      421 aggcataacg caggttagcc aggcgatttc tgaaatggat aaggtgacgc aacagaatgc
      481 ttctctggta gaagaggcct caacggcggc ggtgtccctt gaagaacagg cggcacgact
      541 aactgaggcg gtggacgtat tccgtctgaa caaacattct gtgtcggcag aaccacgagg
      601 agcgggtgaa ccagttagtt ttgctacggt gtgaaaatgt taaaggaggg atcgacagat
      661 cccttcacct ttcagaacgg cattgatttt cgaatagcgt taatcatcaa ctggcaacca
      721 gaagagaacg tcgcatctac gcgggtcagt attccaatcg gttcgcctgc accatgtccc
      781 ggaacaggca gggctaccag cgtggcatga cgcaggtcgt cttttacagc gccagaaggg
      841 acaaaccaca cgtaatcgta ttcaaccgta agttgacgag atagcgaagc agacagcgtt
      901 tcgatacaac ccgaaggaat tttacatccc tggctctgca ctaatgcatc tgaatgctgg
      961 cgtggcgcag tgccttctgg tgatacaacg accggccatt ccagcacccg gcttagcgtt
     1021 acgttctcct gaagtagcgg gtgattaggg cggacaacca gcttcagcga ttcaagaaac
     1081 agtagttcgt aattaagccc gttcatcagt tcaggatctg acatccgacc aatgccgata
     1141 tcgatttccc cggtttttaa acccgccaga atcatagggt tactcattgt cgcaacttgc
     1201 aaggtcgtct ctttttgttg ctgatgaaac tgacctataa ccgaaggtaa tatccctagt
     1261 gccgcagtag gtagcgcacc aaccctgacg acatcattat taagaccttc tttacgatga
     1321 agcgactgtc cggcagtgtt gatggcgtca agaactctga ctgcatgcgt taaaaattgt
     1381 tcgccgggta aggtaagttg cgccccctga cgaccacgct caaacaagcg agcgcccgtc
     1441 agctgctcca gttcattcaa tgtcttagag agcgcaggtt gactcaaatt aagggtttca
     1501 gccgcgcgcc ccaaagttcc ttgttgtgcg acagctacga atgtatgaag gtggcgcaaa
     1561 cggatgcgct gactaaacag actatttttt tccataagcg atgttaaaaa cgaagcggtg
     1621 tcgctgacaa gtgaagttgt tttattatga taacttgatt gcaaaatatt attaacaatt
     1681 aaagcaacta tgttacagca aaatggataa tattgatgtt ttcgcggcga gatcacagtt
     1741 tgtaaattct tccagccgga gtgaatgcgg ttacctacac tccagattac tgaccactgg
     1801 aggcagacac tatggcgaac agcatcacgg cggatgagat tcgggaacag ttttcgcagg
     1861 caatgtcagc catgtaccag caagaagttc cgcagtacgg cacgctgctg gaactggtag
     1921 ctgatgtaaa tctggcggtg ctggaaaaca atcctcaact gcacgaaaaa atggtaaatg
     1981 cagacgaact ggcgcgactg aatgttgaac gtcatggggc gattcgcgtt gggactgcac
     2041 aagagcttgc tactcttcgg cggatgtttg ccattatggg gatgtacccg gtgagctatt
     2101 acgatctctc gcaggcaggg gtgccggtac attcgacagc atttcggccc attgatgctg
     2161 cttctctggc gcgtaatccc ttccgcgttt ttacctcatt actccgcctt gagcttatcg
     2221 agaacgaatt attacgccag aaagcggcgg agattctgcg tcagcgcgat atcttcaccc
     2281 cacgttgtcg acaactgtta caggaatatg agcagcgggg cggttttaac gaaacacagg
     2341 cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg gcaccagtca gcaacggtag
     2401 atgaaaaaac ctatcgcgcc ttgcacaaag aacatcggtt gattgctgat gtggtctgtt
     2461 ttcctggatg ccatatcaac cacctgacgc cacgcacgct ggatattgac cgggtgcagt
     2521 cgatgatgcc tgaatgcgga attgaaccca aaattctgat cgaagggccg ccgcgccgcg
     2581 aggtgccgat tttactacgc cagaccagct ttaaagcact ggaagagacg gtgttgtttg
     2641 cggggcagaa acagggcaca cataccgcgc gctttggtga aattgagcag cgtggtgtgg
     2701 cattaacgcc gaaagggcga caactgtatg atgatcttct gcggaacgct ggaaccgggc
     2761 aggataatct cactcaccaa atgcatttac aggaaacctt ccgcactttt cctgacagtg
     2821 agtttttaat gcgtcagcaa gggctgggat ggttccggta ccgtctgacg ccttcgggtg
     2881 aggcgcatcg tcaggcgatt catcccggag acgatccaca gcccttaata gaacgtggtt
     2941 gggtagtggc gcaacccatt acctatgaag atttcttgcc cgttagcgcg gcggggatct
     3001 tccagtcaaa tctgggtaat gaaacgcagg catgcaatca cggtgatgcc agtcgcgaag
     3061 catttgagca ggcgttgggg tgtccggttt tggatgagtt ccagctttat caggaagcgg
     3121 aagaacgcag taaacgtcgc tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa
     3181 atttgcctga ttttacaaac gaatcaggct catcccatcg acataaaaaa aatgccaatt
     3241 tatgcatatt ctctgatttc aacaattgga ttattaataa atattgtcta gagtgagcgg
     3301 tcataaataa gcactttctt gccgctgaaa atgaccagcg cgggaccatt cacaacacca
     3361 gaaggactca ctttcaggta tggatcgtag acgatttatt aaaggttcaa tggctatggc
     3421 cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct caggcggcat tcgcggccga
     3481 ttctgatatt gccgacgggc aaacccagcg ttttgacttc tccattctac agtcaatggc
     3541 gcacgactta gcgcaaacag catggcgtgg tgcaccacgt ccgttacctg atactctggc
     3601 gacaatgacg ccgcaggctt ataacagtat tcaatacgac gccgaaaaat cgctctggca
     3661 taacgttgag aaccgtcaac tggacgctca gtttttccat atgggaatgg gattccgtcg
     3721 ccgcgttcgt atgttttctg tagatccagc aacacatctg gcgcgtgaaa ttcactttcg
     3781 cccggagttg ttcaaataca acgatgcggg tgtggatacc aaacaattag aagggcaaag
     3841 cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt
     3901 agtatcattc ctcggcgcga gttatttccg cgccgttgat gacacatatc aatacggttt
     3961 gtcggcccgc ggcctggcga tcgacactta caccgacagc aaagaagagt tccccgactt
     4021 taccgccttc tggtttgata cggtaaaacc aggggcaacc acctttaccg tttatgcgtt
     4081 gctcgatagc gccagcatta ctggtgccta taagttcact atccattgtg agaaaaatca
     4141 ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa gacattaaac agctgggcat
     4201 tgcaccgatg accagtatgt tcagctgcgg tactaatgaa cgtcggatgt gcgacaccat
     4261 tcatccgcaa atccatgact ctgatcgtct gtccatgtgg cggggcaacg gcgagtggat
     4321 ttgccgtccg ctgaacaatc cgcaaaaatt gcagttcaat gcttacaccg acaacaaccc
     4381 gaaagggttt ggtttattgc aactggatcg tgatttctcc cattatcagg acattatggg
     4441 ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt aacaagtggg gtaagggcac
     4501 catcggcctg atggaaatcc caactacggg ggaaacgctg gataacattg tttgcttctg
     4561 gcagccagaa aaagctgtaa aagcgggtga tgagtttgca ttccagtatc gtctgtactg
     4621 gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt atggcgacgc gtaccggcat
     4681 gggcggtttc ccggaaggtt gggcgccagg tgaacactat cccgaaaaat gggcgcgacg
     4741 ttttgccgtc gatttcgttg gtggcgatct gaaagctgcc gcgccaaaag gcattgagcc
     4801 ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa attctctata ttgaacccat
     4861 tgatggttat cgtattcagt ttgactggta tccgacttcg gactccactg atccggtcga
     4921 tatgcggatg tatctgcgtt gtcagggcga cgctatcagt gaaacatggt tatatcagta
     4981 tttcccgcca gcgccggata aacgtcagta tgttgatgac cgcgtgatga gttaatcgtt
     5041 ttttcttcgg catcttcttc gggaggtgcc gtctggttaa acacgatccc gctcgcattt
     5101 tttcctaagt taaatgagta atctgctggt gtgtatttca gatacacctt gtcagccact
     5161 aacagggagt gcgtatgttt ccagaatacc gagatttaat atcccgtctg aaaaacgaaa
     5221 atcctcgctt tatgtccttg ttcgataaac acaataaact tgatcatgaa attgccagaa
     5281 aggaaggttc cgacggtcga gggtacaatg cggaagtggt ccgcatgaaa aaacaaaagc
     5341 tacagttaaa agatgagatg ctcaaaatcc tgcagcaggt gagcgtcaaa gaggtgtaaa
     5401 ctttcctaag ccgccagcca ggcggctttt ttaacagctg catggattga ctggagataa
     5461 gatgactgaa acgataaaag taagcgaatc acttgaatta catgctgttg cggaaagtca
     5521 tgtcacactt ctttatcagt taatctgtaa aaataaaacc tggttacagc agtcgctaaa
     5581 ctggccgcag tttgttcaaa gtgaagagga cacgcgaaaa acggtgcagg gtaatgtgat
     5641 gttgcatcaa cgcggctatg ccaaaatgtt catgattttc aaagaagatg aacttatcgg
     5701 cgttatctct tttaatcgta ttgaaccact gaataaaacc gctgaaatcg gctactggct
     5761 ggacgaacct catcaggggc aggggatcat ttctcaggcg ctgcaggcat tgattcatca
     5821 ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa tgtcgggtgg acaatccgca
     5881 aagcaaccag gtagcgttgc gcaatggttt tatccttgaa ggttgcctga aacaggctga
     5941 gtttctgaat gatgcctatg atgatgtgaa cttatacgcg cgtattatcg attcacaata
     6001 accctgccag cagcgttcgc gtaatgcgct cctcgccgtt aatgactttt ggaccacgga
     6061 catgaatcgt gtcgccatca aaggcttcaa cgacggcgtg gtcagttaac tccacatggt
     6121 tttcaattat caccgcacct gtaatacggc tctcaccttg tattacaacg tgctcgtcga
     6181 gcagaatcgg tccgccacgt accacggcat ttccgccaat caagacatga tgttttaaca
     6241 cacaattacc ttccacaatg gcatattccg ccacctgtga actgtaatga atcgtcggaa
     6301 tggcatcttc ttctatgcca gctttcacct gcgcgtgacc gtaaacttta gcgcaatcgc
     6361 atagccagac gttgttctct tcattacctt caatactggc aaaatcaaaa acttcggcgc
     6421 gatgttcaat aaaagcatac cggacgacgg catcgccata aatttgtgcc tggtggacaa
     6481 tacgcgaggc gctaaccttt gcgcgatcat aaatttgtag caggagttga tggtcgggcg
     6541 ttaagccttg tgcggcgacg atcatagaat gttgatcaat taaggcgtga ccaaatattc
     6601 tacattgacc gcaaactaat gagtcactaa tggtgacact gtcacttata tatgcaccct
     6661 ggctgatttc actattatcg atccagacat tatccgttgc gtaaacctct ccccacagca
     6721 cgctggtgcc ggtgatacgc gtattcccgg aaatcaccgt tccgccgaac gcaatagcat
     6781 tctggtcgta aatccagcaa tttccttcct gtgctaacac cgtttcgcga tcgatccagc
     6841 cgccagcgct tcctgctatc acatcgttaa aatcgctcat ggcgataatt tgccgtaata
     6901 acacactttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct tcacttagac
     6961 gatatttacg catagcgttt tcccacaggt gacttactat aaccgtagca aattctgcgg
     7021 ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa
     7081 caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg
     7141 tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca
     7201 ggtcagccac tggttggggg atgggctggt gattctggcg atgatcatct ggggattatt
     7261 gactggcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg cagaagtgcg
     7321 ccatccagtg ctgagcagtt ttgtgagttt gtttccggca acgacgatgc tggtggcgat
     7381 tggttttgtt ccgtggtttc gcccactggc ggtgtgcctg ttcagttttg gtgtcgtggt
     7441 tcagttggct tatgccgcct ggcaaactgc gggattatgg cgtggatctc accctgaaga
     7501 agctaccacg cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc
     7561 ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc
     7621 atggctaagc ctggaaccgg tgatcttgca gcgtctgcgc agttcgggag aattacccac
     7681 ggcactgagg acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg
     7741 gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg gttatggact
     7801 gctgcaactg ctgtttatgc tatgtctgat gccatggtat ctctcccagc catttaatgc
     7861 ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt tgcatctggg
     7921 gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta tctttaccaa
     7981 ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt
     8041 agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgat
     8101 gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt gctggaagcg
     8161 gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt
     8221 ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc
     8281 gccaacgtcg agcgcattaa gtccattgaa aatctggata atttacacac ccgggtcgtt
     8341 gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac tgtggtgctg
     8401 atgttccttg aggctaaaac catcccaggg ctgattgcca atatgcaacg ttgcgctaaa
     8461 cctggcggtt acaacctgat tgtggcggcg atggataccg ctgattatcc atgtaccgtc
     8521 ggcttcccgt ttgccttcaa agagggggaa ttacgtcgat attacgaagg ctgggagatg
     8581 gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg taatcgtatt
     8641 aaactgcgtt tcgccacgat gctggcacgt aaaaaatgat ccggtaagaa caaagcgcgt
     8701 gaaaattccc cacgctgaga tggtttactg ttcttctttt cggtaagcat attttttatc
     8761 gaagggatgt gaaattaatc acagtagtcg aagtttttta gccgcttaac gtactgaaat
     8821 ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt ctgaaaaggg
     8881 gctaaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg cgctttttgc
     8941 tataacttaa aaagtaatat aatcatctca ggaaactatt catgcgtacc acatcatttg
     9001 cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc
     9061 agccagataa atattctggt tttttaaaca attactctga tttaaaagaa acaacctcgg
     9121 caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc aaatatgaca
     9181 gcatcgtctg gaacccaatc acttattatc cggtaccgaa accttctacc caggtagggc
     9241 agaaagttct ggataaaatt ttgaactata ccaacaccga aatgaaagaa gcgatagcgc
     9301 agcgtaaacc actggttacc accgctgggc cgcgtagtct gattttccgt ggggccatta
     9361 ccggtgtaga taccagcaaa gaagggctgc aattctatga agtggttcct gttgcattag
     9421 tggttgcggg gacgcaaatg gctacaggcc accgtaccat ggatactcgc ttgtattttg
     9481 aaggtgagct gattgatgca gcgactaata aaccggttat caaagtcgtt cgtcagggcg
     9541 aaggtaaaga cctgaataac gaaagtacgc caatggcttt cgaaaatatt aaacaagtta
     9601 ttgatgacat ggcgaccgat gccaccatgt ttgacgttaa caaaaagtag ttccagacgc
     9661 gccatcgtta gatggcgctt tttattatcg gtttctgtta gcgattaaat tcaacaccac
     9721 gtaacaaacg cctccggcaa ttaatcccca aaacgcagaa ccaatcccga acagcgtcaa
     9781 tccacttgcc gttaccagaa atgccaccac cgccgcgtcg cgctcacgct cattatgcag
     9841 cgcctgatac aaactgccgc caatggtact caacagcgca agacctgcca gcatctggat
     9901 ccaacttacg ggcagggcag ccatcatccc ggtaatggca ctaccaaaca gacctgcgat
     9961 caaataaaaa atgccggcaa cggcagcggc cagccaacgt tgatctttat ccggatgcgc
    10021 ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa
    10081 aggggaaaaa aacagtgcca gcaatccagt aaacacaatc aacggcgaaa caggagccga
    10141 atacccggct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
    10201 aaaaaggggg agtgcaacgc tcagactgtg agcaaacgaa aaatcagggg taatataagt
    10261 ggggagaacg ggtttaaaga caatatcagt tgtaacaacg tcaccttgcg cggtaacgat
    10321 gacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccttggctgc
    10381 cagccatgcc agcaacatac tgccacacaa cgtaaattga ccatccagac tggcaaacgc
    10441 ctgtaaacca aaacgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
    10501 aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta acgcgttggt
    10561 gacaataaaa acgccgatgg cttcgttaag tgttagttcc tgcaatccgg tgaccaacaa
    10621 agccgcgcca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagt
    10681 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
    10741 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
    10801 taataccgcc agaaaccccg ccagtagcgt gggtggagga atagaaaaca gacgcatagt
    10861 cacctcgtgc gttataacgt acaggtaccg tagcatttgt ccgttataac gcacaagtga
    10921 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgttttt tatccaccac
    10981 acttaaacaa ctacgtcagc agcgcggctg gagtctttcg cgactggcag aagcgacggg
    11041 cgtttctaaa gcgatgttag ggcagattga gcgtaatgaa tccagcccga cggtcgcgac
    11101 attatggaaa attgccaccg ggttgaacgt gccattttca acatttattt ctccgccgca
    11161 gtccgctacg ccttcagttt atgatccaca acggcaggcg atggtgatca cttcgctgtt
    11221 tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcac ccggtgcaat
    11281 aagcgaatcg atgccccatg aaaagggggt aatcgaacat gtcgtcgtca ttgatggaca
    11341 acttgatctg tgtgttgatg gtgaatggca gagccttaat tgtggggaag gcgttcgatt
    11401 tgctgcagac gtcacgcatg tctatcgtaa tggtggggag caaaccgtac attttcattc
    11461 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
    11521 taaaatagcc gccatttttc agctactgga taagaaagtg accgtatctt ctcatcgtct
    11581 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
    11641 cgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagcct
    11701 gaaagatatt gccgagttgg tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac
    11761 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttactgacct
    11821 ctaccagacc ggtgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
    11881 tccgccgatt gaactgcacg ccagtacgca gtgcgatatt cgtacagtag aaaaagcgaa
    11941 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagaactga atcttgagca
    12001 gatccgcgcg attcaccagg ccacgaacgc gaccattgaa ttctttattc atggcgcatt
    12061 gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc gtagcgccaa
    12121 ccgtggcgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
    12181 ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga ctgccaacct
    12241 cggcgcgctg attgatgctg gcgtacgctc cttcaagatt gaagggcgtt acaaagatat
    12301 gagctacgtg aagaatatca ccgcccatta tcgccagatg ctggatgcca ttattgaaga
    12361 acgtggcgat ctggcgcgcg cttcatcagg tcgtactgaa catttctttg ttccatcgac
    12421 ggaaaagact ttccaccgtg gtagcacaga ttattttgtg aatgcccgta aaggcgatat
    12481 tggcgcgttc gattcgccga aatttatcgg cctgccggtg ggcgaagtat tgaaagtagc
    12541 gaaagatcat attgatgttg ccgttaccga gccactggca aatggcgatg gcctgaacgt
    12601 gatgattaaa cgtgaagtcg tcggttttcg tgccaatacg gtagagaaaa ccggagaaaa
    12661 tcagtaccgc gtctggccaa atgaaatgcc agcagatttg cacaaaattc gcccgcatca
    12721 cccactaaac cgtaatcttg atcataactg gcaccaggca ctgacaaaaa cctccagcga
    12781 acgtcgggtg gcggtagaca ttgaactggg cggctggcag gaacaactga ttctgaccct
    12841 gaccagtgaa gagggcgtca gcgtcacgca tacgctggac gggcagttcg acgaagccaa
    12901 taacgccgaa aaagcaatga acaatctgaa ggatggtctg acaaaactgg ggcaaaccat
    12961 ctattacgcc cgcaatgtgc aaattaattt gccgggggcg ctgtttgtac caaacagtct
    13021 gttaaaccag ttccgccgtg aagccgccga tatgcttgat gctgcgcgtc ttgccagtta
    13081 ccagcgcggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc aaacgcatct
    13141 gagtttcctc gcgaacgtat acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt
    13201 gcaactgatt gacgcggcgt atgaggcaca tgaagagaag ggcgaagtcc cggtgatgat
    13261 caccaagcat tgtctgcgct ttgccttcaa tctgtgcccg aaacaggcga aaggcaatat
    13321 caaaagctgg aaggcgacgc caatgcaact ggttaacggc gatgaagtat taacgctaaa
    13381 gtttgattgc cgcccatgcg agatgcacgt cattggcaaa atcaaaaatc acatactgaa
    13441 aatgccgtta ccgggaagcg tagtggcatc cgtaagtccg gatgatctgc tgaaaacatt
    13501 gccgaagcga aaagggtaaa acgccagttt tcgggttact caccactcat caaatctgca
    13561 tgatattgcc tgccgggtaa ggcgtcatgc cgcatccggc aacaaatgac tcaacgctgt
    13621 cccgcttctg gtttgcgcga tttttgccag taaaaatgct cgcgcaaacc ttccgccgac
    13681 tcttccgcca cagcacgcaa ttcatcgctg tccgcttcat gacgcagctg atgatccaca
    13741 ttctttaccc acataaattc atgtcctttg tgccctgcca tgagttgtcc tgagaacagt
    13801 gcacacgtta ataagacaac cgataacgcc ttcgaaaaca tcccgccacc tttttctaac
    13861 cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg caaattagag
    13921 caaagaaatt ctggaatctt ccttcctgat tttgcattga attctgccgt tgcggcgatt
    13981 tagtgctatt ttcgagcaca ttacacacgg aggtaaactt cgccattaaa tagtcacgat
    14041 cactttattg aagtgccttt gagcgtcgcc tctaatgtat tgctgttaaa tgctttttta
    14101 cagtcagaaa tcactcagca agagttagcc aggcgaattg gcaaacataa acaggagatt
    14161 actcgcctat ttaatttgca ccatgcgaca aaaatcgacg ccgtccagct cgcggctaag
    14221 gcgcttggca aagagttatc gctggtgatg gtttaattac agttaacgaa aagttgtcat
    14281 ttttaacaac tgatatagac tgccgaatca tctgcacata attacgattc gataatgaaa
    14341 aaataccagc agcttgcaga acaattacgc gagcagattg cgtcgggtat ctggcaaccc
    14401 ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt ctggcatgag ctttatgact
    14461 gtcagccatg cctatcagtt gctcgaaagt cagggatata ttatcgcgcg accgcaatcg
    14521 ggttattacg ttgcgccaca ggcaataaaa atgccgaaag cgacagtcat tccagtcaca
    14581 cgagatgaag cagtcgatat caacacctat atttttgata tgttgcaggc cagtcgcgat
    14641 ccgtcggtag ttccgtttgc ctcggccttt cccgacccgc gacttttccc cctccaacaa
    14701 ctaaaccgct cgctggcgca ggtaagcaaa accgccacgg cgatgagcgt gattgaaaac
    14761 ttaccgccag gaaacgcaga actgcgtcag gctattgctc gtcgctatgc cttacagggc
    14821 atcaccattt ctcctgatga aattgtcatt accgccgggg cgttagaggc attaaacctc
    14881 agtttgcaag cggtaactga accgggcgat tgggtgatag tagagaatcc ttgtttctac
    14941 ggcgcgttgc aggcgctgga gcggttacgg ctgaaggcgt tatcggtagc gacggatatt
    15001 aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc aggagtatcc ggtgaaagcg
    15061 tgctggctga tgactaatag ccagaaccca cttggattta ccttaacgcc gcagaaaaaa
    15121 gtgcggctgg tagcgttgct caatcagtac aacgtaacgc tgattgaaga tgacgtttat
    15181 agcgaacttt attttggacg ggaaaaaccg ctgcctgcga aagcgtggga tcgccacgat
    15241 ggtgttttgc attgctcttc gttttcgaaa tgtctggtgc ctggttttcg tattggttgg
    15301 gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc agttgatgag tacgctttcc
    15361 accagctcac cgatgcagct tgcgctggtg gattaccttt ctactcgccg atacgatgct
    15421 catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac aacgcgcctg gcaggctttg
    15481 ctgcgttatc tgcctgcgga agtcaaaatt catcacaatg acagcggtta ttttctctgg
    15541 ctggagctcc ccgagccgtt agatgcagga gaattaagcc tggtggcact gacgcatcat
    15601 atcagtattg cgccgggtaa tatgttttct accggtgaaa actggtcacg tttttttcgt
    15661 tttaataccg cgtggcagtg gggagaacgt gaagaacagg cggtaaaaca attaggcaaa
    15721 cttattcaag aacggctgta atagcgttta atttaattcc tctcaggttg ggtaatatga
    15781 atttcgaata gcagtcatat atcctaactc cttgtctata ctccagaaga taaccttaca
    15841 gacggcataa tgcgcggtag ctcacaacct gattaaattt tctcagggcc gaaggtgtgc
    15901 ctgcaagccg ccgtctatgg ttaaacaagg agatattttt acggcacggc ggctgaacaa
    15961 ttaattacga caggagtaag accttatgag caagacattt gcccgcagca gcctgtgtgc
    16021 gctcagcatg acaataatga ccgctcacgc cgccgaaccg cctaccaatt tagagaatcc
    16081 ggaagggcga ctggatatta tcgcctggcc gggatacatc gaacgcggac aaactgataa
    16141 acagtacgac tgggtaacgc aattcgaaaa agagacaggc tgcgcggtga atgttaaaac
    16201 cgccgcgact tccgatgaaa tggtcagtct gatggccaaa gggggttacg atctggttac
    16261 ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa cgcgtgcagc caattaatac
    16321 cgccttgatt cccaactgga aaacgctcga tccgcgcgtg gttaaaggcg actggtttaa
    16381 cgttagtggc aaagtttatg gcacacctta ccaatggggt ccgaacctgt tgatgtacaa
    16441 cactaaaacc ttcccgacgc cgccggatag ctggcaagtg gtttttgttg agcagaatct
    16501 gccggacggc aagagcaata aaggccgcgt tcaggcttat gatggcccta tctacattgc
    16561 ggacgctgcg ttgttcgtta aagccactca gccgcagttg ggcatcagtg atccgtatca
    16621 actcaccgaa gaacagtacc aggcggtgct gaaagtgctg cgcgatcaac atagtttgat
    16681 ccatcgctac tggcatgaca ctaccgtgca aatgagcgat ttcaaaaacg agggtgtggt
    16741 tgcttccagt gcatggccct atcaggccaa cgccctgaaa gccgaaggcc agcctgtcgc
    16801 taccgttttc ccgaaggagg gcgttaccgg ttgggctgac accaccatgc tacatagcga
    16861 agcgaaacat ccggtttgcg cctacaaatg gatgaactgg tcattaaccc caaaagtgca
    16921 gggcgatgtg gcggcctggt ttggctcgtt accagtagtg ccgcaagggt gtaaagccag
    16981 tccgttatta ggcgagaaag gttgtgaaac aaacggtttt aactatttcg ataaaatcgc
    17041 cttctggaaa acgcctgtag cagaaggggg caagtttgtt ccctacagtc gctggacgca
    17101 ggattacatt gccattatgg gcggtcgcta acttcgctgg ggtgttttat gacgtacgca
    17161 gtggagtttg acaacgtctc acggttgtac ggtgacgtgc gcgcagtaga tggcgtcagt
    17221 attgcgataa aagatggtga gttcttctct atgctggggc cgtccggctc cggcaaaacc
    17281 acttgcctgc gcctgattgc tggattcgaa cagctttccg gcggggctat ctctattttt
    17341 ggtacgcccg ccagcaatct gccaccgtgg gagcgggacg tgaatactgt cttccaggac
    17401 tacgcgctat ttccgcatat gtcgattctt gacaacgtag cctacgggct gatggtcaaa
    17461 ggcgtgaata aaaagcagcg gcatgcaatg gcgcaagagg cgcttgagaa agtggcgttg
    17521 gggtttgtac agcaacgtaa accgtcacaa ctttctggtg gtcagcgcca gcgggttgct
    17581 atcgccagag cactggtgaa cgaaccgcgt gtgttgttgc tggatgaacc gctcggcgcg
    17641 ctggatctca aattgcgaga gcaaatgcag cttgagctga aaaagctgca acagtctctc
    17701 ggtatcactt ttatcttcgt tacccacgat cagggcgaag cgttatcgat gtccgatcgg
    17761 gtggcggttt tcaataatgg acgcattgaa caggtcgatt ctccgcgcga tctctatatg
    17821 cgcccgcgca cgccgtttgt tgcgggattc gtcgggacat ccaacgtttt tgatccgcta
    17881 atggccgaga aactttgtgg catgacggga agcttcgcac tacgaccgga acatatccgc
    17941 ctcaacaccc atggcgaaat gcaggctaat ggcacgatcc aggcggtgca atatcagggc
    18001 gcagcgaccc gttttgaact gaaattgaac ggcggtgaaa aactgctggt gagtcaggcc
    18061 aatatgacag gcgaagagct acctgccacg ctcacgcccg gacaacaggt gatggtttcc
    18121 tggtcacgtg atgtgatggt ggcgctggtt gaggagaggt gaatggcgat gaacgtattg
    18181 caatcacctt cacgtccagg tctgggtaag gtgtccggtt tcttctggcg taatccgggg
    18241 ctggggctgt ttttgctgct gcttggcccg ctaatgtggt ttggcattgt ctatttcggc
    18301 tcgctgctga cgctgttatg gcagggcttt tatacctttg acgatttcac catgtcggta
    18361 acgccggaac tgacgctggc gaatatccgc gcgctgttta atccggcgaa ttacgacatc
    18421 attgtccgca cgctgaccat ggctgtggca gtcactatcg ccagcgccat tctggctttt
    18481 ccaatggcgt ggtatatggc gcgctatacc agcgggaaaa tgaaagcgtt tttttatatc
    18541 gcggtaatgt tgccgatgtg ggcgagctac attgttaaag cctatgcctg gacgttattg
    18601 ctggcaaaag atggcgtggc gcagtggttt ttacaacatc tggggctgga accactgctg
    18661 actgcgttcc tcacgctacc ggcggtggga ggtaatacgc tgtcaacttc cgggctgggg
    18721 cgctttctgg tgtttctcta tatctggttg ccgttcatga tcctcccggt gcaggcggcg
    18781 cttgagcgtt tgccgccgtc attgttgcag gcgtcggctg atcttggcgc acgtccacga
    18841 caaacctttc gctatgtggt gctgccgctg gcaatcccgg gtattgccgc tggctctatc
    18901 tttaccttct cactcacgct gggcgatttt attgtcccgc agctggttgg ccctccagga
    18961 tattttatcg gcaatatggt ttattcccag cagggggcga ttggcaatat gccgatggcg
    19021 gcggcattca ccctggtgcc gattgttctc atcgcacttt acctggcgtt cgtgaaacgt
    19081 ctgggagcgt tcgatgcact ctgaacgcgc accgtttttc ctcaaactgg cggcctgggg
    19141 cggcgtggtt ttcctacatt ttcccatcct gattatcgct gcctatgcgt ttaacactga
    19201 agatgcggcg tttagttttc caccgcaagg cctgacgctg cgctggttta gcgtggcagc
    19261 acagcgtagt gatatccttg atgccgtgac actgtcactt aaagtggcgg cgctggcgac
    19321 attaatcgcg ctggtgttag ggacgctggc agctgccgcg ctgtggcgac gagacttttt
    19381 cggcaaaaac gccatttcgc tgttactgtt gctgcccatt gcgctgccgg gcattgtcac
    19441 tggtctggcg ctattaaccg cctttaaaac catcaatctg gagccaggat ttttcaccat
    19501 cgtggtcggt catgcgactt tttgcgtagt ggtggtgttt aacaatgtca tcgcccgttt
    19561 tcgccgcacc tcgtggagtc tggttgaggc gtcaatggat cttggggcca atggctggca
    19621 aaccttccgc tacgtagtgt tgccgaatct cagttcggcg ttactggctg gaggaatgct
    19681 ggcgtttgcc ttgtcgttcg atgaaatcat cgtgacgact tttacggcag gtcatgaacg
    19741 aacgttaccg ttgtggttgc tcaatcagct tgggcgaccg cgtgatgtac cggtaaccaa
    19801 cgtggtggca ctgctggtta tgttggtaac aaccttgccg atcctggggg cctggtggct
    19861 aacccgcgaa ggcgacaatg gtcaataacc actgatacag gaatatgcta tgcaacataa
    19921 gttactgatt aacggagaac tggttagcgg cgaaggggaa aaacagcctg tctataatcc
    19981 ggcaacgggg gacgttttac tggagattgc cgaggcatcc gcagagcagg tcaatgctgc
    20041 tgtgcgcgcg gcagatgcag catttgccga atgggggcaa accacgccaa aagcgcgtgc
    20101 ggaatgcctg ctgaaactgg ctgatgttat cgaagaaaat ggtcaggttt ttgccgaact
    20161 ggagtcccgt aattgtggca aaccgctgca tagtgcgttc aatgatgaaa ttccggcgat
    20221 tgtcgatgtc tttcgctttt tcgccggtgc ggcgcgctgc ctgaatggtc tggcggcagg
    20281 tgaatatctt gaaggtcata cttcgatgat ccgtcgcgat ccgttggggg tcgtggcttc
    20341 tatcgcaccg tggaattatc cgctgatgat ggctgcgtgg aaactggctc cggcgctggc
    20401 ggcagggaac tgcgtagtgc ttaaaccatc agaaattacc ccgctgaccg cgttgaagtt
    20461 ggcagagctg gcgaaagata tcttcccggc tggcgtgatt aacgtgctgt ttggcagagg
    20521 caaaacggtg ggcgatccgc tgaccggaca tcccaaagtg cggatggtgt cgctgacggg
    20581 ttctatcgcc actggggaac acatcatcag ccataccgcg ccgtccatta agcgtactca
    20641 tatggaactt ggtggcaaag cgccagtgat tgtttttgat gatgcggata ttgaagctgt
    20701 ggtcgaaggt gtacgtactt ttggctatta caatgccgga caggattgta ctgcggcttg
    20761 tcggatctac gcgcaaaaag gcatttacga tacgctggtg gaaaaactcg gtgctgcggt
    20821 ggcaacgtta aaatcgggtt cgccggatga cgagtctacg gagcttggac ctttaagctc
    20881 gctggcgcat ctcgaacgcg tcagcaaggc ggtagaagag gcgaaagcga cagggcacat
    20941 caaagtgatc actggcggtg aaaagcgcaa gggtaatggc tattactatg cgccgacgct
    21001 gctggctggc gcattacagg atgatgccat cgtgcaaaaa gaggtatttg gtccggtagt
    21061 gagtgttacg ctcttcgaca gcgaagaaca ggtggtgaac tgggcgaatg acagtcagta
    21121 cggacttgca tcttcggtat ggacgaaaga tgtgggcagg gcgcatcgcg tcagcgcgcg
    21181 gctgcaatat ggttgtacct gggtcaatac ccatttcatg ctggtaagtg aaatgccgca
    21241 cggtgggcag aaactttctg gttacggcaa ggatatgtca ctttatgggc tggaggatta
    21301 caccgtcgtc cgccacgtca tggttaaaca ttaaggataa tattgcagat cgtaagagta
    21361 tgagatgatc ttgcgtactg tcagagcact gatttctctg acagtacgtg ctgcgtgcaa
    21421 aatcactaca tcaaataaac cagccaaatc tcatagagat aagagtaaac aaaccaacag
    21481 cagcaaagac atttatcagt actacagttc tactggaaac attcattttt gccacctttt
    21541 atcattgccc ctatgtgtat agtacgggtt aagaaaatcc gcaataatac tcattctgaa
    21601 gatggataag ggcaagttgc tgtttgatga ttttttctca aactggtgaa aaaaacttga
    21661 tgcacgtcaa aaaatgacgc atatttgcgc gcgttttatt catctggctg gacgcccgta
    21721 catgtctctc tatcaacaca tgcttgtttt ctatgcggtt atggcagcaa tcgcatttct
    21781 tatcacctgg tttctttctc atgataagaa acgcatccgt ctcttaagcg cctttctggt
    21841 gggggcaaca tggccgatga gtttcccggt ggcgctgttg ttttctctgt tttaaatctt
    21901 atgcactggc ttgacggcac cacgcagact tatatcattt ggatgaatcg ataaatttca
    21961 caagtggcta aggagaaagt atgtcgcatc tggatgaagt catcgcgcgc gtggatgccg
    22021 cgattgaaga gagcgtcatt gcccatatga acgaattatt aatcgccctg agcgatgacg
    22081 cggagttaag tcgggaagat cgctacaccc tgcagcagcg tttgcgcacc gctattgctc
    22141 atcacggtcg caaacataaa gaagatatgg aagcgcgcca cgaacagtta accaaaggcg
    22201 gtaccatcct ctgattaaaa tgagcgtctg gccaccagcc aggcgccaac cacaagtaaa
    22261 attgcgccac aaattgggcc gatcaacgcc cgaagtggta cgccatggct gcggaagata
    22321 tccagcacca gaccgccaat cagctggctg gcgaccagta ctgcaatagt tgtcgccgct
    22381 cccacattct gatatccgct gatactggcg aagacaaaaa acgatcccag caagccaggt
    22441 ataagtgtcc accagcgcac gctggacacc agttcgccaa accccgccat gccttgttta
    22501 aaccataaaa tagaaacaaa gagcacaatc cccaccagtg agttcagcaa catggcgatg
    22561 agaatggtag aggaggtctg ggtgatgcgc accatcagcg tgttctggac taccagacca
    22621 atcccggctg caatcaaaaa ggcaagggta agtgactgat tcatccaatc gcgtccggtt
    22681 cagtgcgctc gtcgagttgc aactgcataa atgtcagatc cagccagcgc ccaaatttag
    22741 tgcctacctg cggcatttgc gcggtgacga caaatcccag cgactggtgc agatgcagcg
    22801 aggcctgatt ttgcgattcg atcccggcga ccatgacatg cttcccgcaa tcccgcgctt
    22861 catcaattaa tcggcttaac aatttacgcc ccagaccttt cccctgatga tcgggatgga
    22921 cataaaccga atgttccacg gtatggcgaa aaccatcaaa actacgccag tcgccaaacg
    22981 aggcatatcc cgtcactacg ccatcttctt cactcaccag caccggataa cctgctatgg
    23041 tccgcgcttc aaaccaggca atgcggttat ccgcatccac cgtttggtca ttccagatag
    23101 ccgccgtata caacacggcg tggttataaa tttccgcaat tgcagcacag tcggctttgc
    23161 gggcaaaacg gatggacatg tctggcctcg ataaaataat gatgatgatg ttatcaatac
    23221 tatcgaaaac agcccgaagc aacggattcc tctatgaaat tattgcgtgt tgcagagggg
    23281 atgtgacggc tgcaaacaaa accaatcctg tgagcctgct actattgatg gattcatgcc
    23341 aaaagcacag gagagcatta tggggcaaca aacgcagcgt aaccgatgtt gggttctgtc
    23401 ctcgcgtccg catggcgcgc cggttcctta cgtctggttt cggtaaatgc gctctttgtc
    23461 gcaccagcgc aagcatttac agttccggaa gtgggaaaac gtcaattaaa tgaaatcaat
    23521 cggatccgct acgaactgga gttaatggca gttgctctgg ctgtcgaaaa cctcactatg
    23581 caaaaaaacg ttgctatgtt gtgccagcaa tacagccgat aaataaaata cgcccggcaa
    23641 ataccgggct taaagataat tactcaaatc gccacgcaat atttatgccg acaccataat
    23701 ttcgtccggg cgcaggttcg taatatcgcc cgtttgactc attgacaatg acagaaccaa
    23761 cgtattcttt atcgaataaa ttatcgacac gaccaaataa atccacggtt aaattgtggt
    23821 aattgtattt atacccggtg aataagccga cgagtgtata agaaggcgct ttggccgtat
    23881 tttcatcatc tgccataata tcgcccatat aacgcgcttc cgtgcctgca taccaaccat
    23941 cttccggtat ataacctatc gacgcaaagc ccatattacg ggcgatccct ggcatccggt
    24001 taccgttaca atcctgttca ttgcagacat tgctgcgata ggtcgcatca agccaggtcc
    24061 aggacgcttt tacgcgaaaa tctcccgcga aacgttgatc ccatgccagt tcagcgcctt
    24121 gacgacgggt ctttccggca tttttgtaag tcgtacgccc accgctactg ctatcgacaa
    24181 caatttcatc atcggtgtcg gtctggaaca atgcgagact aagcagtcca tcaccaatac
    24241 gcgttttact gccgatctca attgtatcgt tggtggaggg ttttaaacca aagttcatac
    24301 cgctttgtcc atcagcgcga taagacagtt cattaatcgt cggcgtttca aaaccgcgcc
    24361 cggctgccag atagacattc caggcatcgg tcattgcata ttttaacgat ccggcaggta
    24421 gccatttgtg ataactggca tcaccgctgt catcgccgtt acccggagta acgtaatgat
    24481 cgttggaatc aaaccacacg gagctgtagc gcacgccagc atccagcgaa agtttgtcgg
    24541 tcagttgcca ctgggtttgt aaatagggat cgacgttcca catcaaattg cgttcgtcgc
    24601 ggcgcaactc acctttttgc ccgtattccg gcacgccgcg attcaggcgg aagttattgt
    24661 aacccttgcg gttttcactc atgttttcgt agttcaggcc ggttgtgaac gtgactggaa
    24721 cgcccagttc accacggtgt gtccagcggc tgtctatccc ctggtaatgg cgttgcaggg
    24781 taatcacgcc gcccgcatgt gacgggttaa gttgcggggc catggggatt gactggtact
    24841 gggtcgtttc tcgctctccg gcatacatca tcacactcat atcatcttgt gcgctcaggc
    24901 tacgctcata gcgcaaccca gcctgagttt gcttgatggt ttttcgcatg tcgtactgtt
    24961 ctgcacgagg cgcttgttgt ggattcgcct tccattctgc ttcggttagc ccacctgggt
    25021 catctgcttt gatatccaca ctattgaaaa tcaggcttaa tttgctggcg tcatcaatgc
    25081 gtacgcccag tttggcattg gctaaatttt tctgtgcgcc actatggtca cgatagccgt
    25141 gggtcgtaaa acgtgtggtt gcgacggtgt aatcgacatc gccgggttgt gtgccgtcgc
    25201 ccgttgcgcc cgttgctttc agcccatagc gccagctgcc aaaactgccg tagtaactac
    25261 tggcttcaat ggttggtggc tgttgtccgg tctgggtggt gacattcatt acaccgccag
    25321 acgcgttgcc atacagagca gagaaggggc cacgcagcac ttccacattt tgcacactgc
    25381 ttaaatcgat gttagatgtc tgcccttgtc cgtcgggcat ggtagcggga ataccgtcca
    25441 catacaggcg aataccgcga attccgtaag tggagcggga gccaaatccg cgaatcgaca
    25501 gctgtaaatc ttgcgcatag ttctgtcggt tttgtacctg taaaccaggc actccggtca
    25561 gtgattcaga caagttaatg cgcggtgttg ccaggcgcat ctcctcgcca tccaccacgc
    25621 tcacggctgc tggggtatcc agttctgaaa ccacctgcgg tgcggcactg acaatcatgg
    25681 tctgttcatc agcggcaaaa acaacggggg aaaggacaag cagtgcgggc aaaacggtct
    25741 gtcggacgga aaaaatcttc atgaaaaaag ccaggttaag aatgggaaaa caccgtcatg
    25801 gtaataaaat tgtaaattta tggaaaacaa aacggcacaa tacgttaagt aattgagaaa
    25861 attgtagtca taacggtaag aaatgctcca catttgagaa aataatgatt accattccca
    25921 tttataacaa gggcgtaacg atgattacgc ttagcgaagc attgtgaagc agcaaaaata
    25981 tcggttcatc aaagggagtc gtcatgcatt tacgtcatct gttttcatcg cgcctgcgtg
    26041 gttcattact gttaggttca ttgctcgttg cttcatcatt cagtacgcag gccgcagaag
    26101 aaatgctgcg taaagcggta ggtaaaggtg cctacgaaat ggcttatagc cagcaagaaa
    26161 acgcgctgtg gattgccact tcgcaaagcc gcaaactgga taaaggcggc gtggtttatc
    26221 gtcttgatcc ggttactctg gaagtgacgc aggcgatcca taacgatctc aagccgtttg
    26281 gtgccaccat caataacacg actcagacgt tgtggtttgg taacaccgta aatagtgcgg
    26341 tcacggcgat agatgccaaa acgggcgaag tgaaaggacg tctggtgctg gatgatcgta
    26401 agcgcacgga agaggtacgc ccgttgcaac cacgtgagct ggtagctgac gataccacga
    26461 acaccgttta catcagtggt attggtaaag agagcgtgat ttgggtcgtt gatggcgaga
    26521 atatcaaact gaaaaccgcc atccagaaca ccggtaaaat gagtaccggt ctggcgctgg
    26581 atagcaaagg caaacgtctt tacaccacta acgctgacgg cgaattgatt accatcgaca
    26641 ccaccgacaa taaaatcctc agccgtaaaa agctgctgga tgacggcaaa gagcacttct
    26701 ttatcaacat cagccttgat accaccaatc agcgtgcatt tatcaccgat tctaaagcgg
    26761 cagaagtgtt agtggtcgat acccgtaatg gcaatattct ggcgaaggtt gcggcaccgg
    26821 aatcactggc tgtgctgttt aacccagcgc gtaatgaagc ctacgtgacg catcgtcagg
    26881 caggtaaagt cagtgtgatt gacgcgaaaa gctataaagt ggtgaaaacg ttcgatacgc
    26941 cgactcatcc gaacagtctg gcgctgtctg ccgatggcaa aacgctgtat gtcagtgtga
    27001 aacaaaaatc cactaaacag caggaagcta cccagccgga cgatgtgatt cgtattgcgc
    27061 tgtaatagat atggcgaggg tgaaaacgga aagaattgcc tgacttttat cacgccgcat
    27121 ccggcacctt cgtcagtagt ctgaatttgc ccggcacgta gccgggcaat tttgcaatta
    27181 actcgccgtt tcaggcttaa acacaatgtc ctgtttttct tcatcttcct cgacgactgg
    27241 cgcagtgctg tgaatttcag caacgcgttt gcgcacacca aaccagccta taaccagcag
    27301 aataccgata atcggcagcg ccgcgatggt ataagtcccg ttcgggtaat cgaacgccat
    27361 cagcaccagg acactcagta aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg
    27421 cagtttaaaa ctgacatccg ctgctttgcc ttctttaatc gctttacgca ggcgcatctg
    27481 gcaaacgatg ataaacgccc atgaagcgat gattcccagc gacgcgaagt tcaacacaat
    27541 ctcaaatacg cgtgacggca ccagatagtt gaggaatacg ccgacgacat acacaaccag
    27601 tgtcgccaga atcccggcat acggcacatg ctgacgactc attttcgcca taaaactcgg
    27661 tgcggaaccg cccatcgcca tcgagcgcag aatacgcccg gtgcagtaca gacctgaatt
    27721 caggctggag agggcagcgg tcagcaccac aatgttcata atgctgccta tatatggcac
    27781 acccagttta gagaaaaacg tcacgaacgg actttgcccc gcctgatatg cgctccacgg
    27841 caataacata accagcaaca ccacggagcc gacgtaaaac agaccaatac gccaaatcac
    27901 actgttaatc gctttaggta ccatggtctg cggatcttta cattctccgg ccgctgtacc
    27961 caccatttca atggaggcaa aagcaaacac tacgccctga atcaacacca gcgcaggcag
    28021 caaaccgtgg gggaagaagc cgccattatc ggtgattaaa tgaaagccag tggtgttgcc
    28081 atccagcggc tgaccgctac cgaggaacac tgtacccacg accaaaaagg ttacaatggc
    28141 gagcacttta ataagcgcaa accagaactc catctccgca aaccatttca caccgatcat
    28201 attcatggtg ccaacgatgg tcagtgcagc gagcgcaaag acccactgcg gcacgccgcc
    28261 aaacgcgccc cagtaatgca tatacagagc gacggcggta atatcaacaa tccccgtcat
    28321 cgcccagttg atgaagtaca tccagccagc aacataagcg gccttctcac ccaaaaactc
    28381 acgggcataa gagacaaaac tgccactgga agggcggtgt aacaccagct cacccaatgc
    28441 acgcaaaata aaaaacgaaa acaggccgca gatcaaatag accagtgcca gtgcgggccc
    28501 cgccatttgc agtcgcgccc ccgcgcctaa aaacaaaccc gtaccaatcg cgccgccaat
    28561 ggcgatcatc tgtacctggc gattgcccat cgctttgtga tacccctctt cgtgggcatt
    28621 aagccagcgg cgtttcgcgg cgtgttgatc tgaagtgtcg gtgttgtgtt tactcattgc
    28681 tctccctgat tgctttaatg aaaaagtcat ataagttgcc atgaacaatg tttattcatc
    28741 ctggcgatga caagattcac cattattctt tatgggtata actgtcggcg cagaatccta
    28801 ccttttatca tgtaacgatg caaaataatc ccgctataaa tatgtgggga taaagtcggc
    28861 tggcgacaaa atagcaacct gatgaaatta tgacggaaac aaccactatt ggtttgatga
    28921 tacctgactg cagtcctact cttgcgccac tcgttcgtcg ctctgaacgc cagtggttgc
    28981 agtggacact ggcgtggttg gctcaatgaa tagtgagggg ctagcacatg atgggtttca
    29041 ggggtaatac gccaaatacc aatacaattg ctgattaaat gagccaattg ccgacattac
    29101 agaaaaggct aaaaaatgta agaggcatgt agggtgataa acgcgctatt gcccaacagg
    29161 agtttctgga attagtcctg ttccatgggc attacaactc ttaaaacatt acttcaatat
    29221 aattaatgtg tatatcattc tcaatattaa tttatgaagt gaatttaata tattccgaat
    29281 tcttaattta cattactaat gtatggggca taacgttaag atggctatgt gcagattaag
    29341 atatcaggga aagatcacgt aacgctgctt ttttgtacta aataattcgc attttatgtt
    29401 taaaaattga gatattcctc attacctgaa gctgtttttt attgcttata catgatcaaa
    29461 tactccttac ataattaagg agaacaaaat ggaacttaaa aaattgatgg aacatatttc
    29521 tattatcccc gattacagac aagcctggaa agtggaacat aaattatcgg atattctact
    29581 gttgactatt tgtgccgtta tttcgggtgc agaaggctgg gaagatatag aggattttgg
    29641 ggaaacacat ctcgattttt tgaagcaata tggtgatttt gataatggta ttcctgttca
    29701 cgataccatt gccagagttg tatcctgtat cagtcctgca aaatttcacg agtgctttat
    29761 taactggatg cgtgactgcc attcttcaga tgataaagac gtcatcgcaa ttgatggaaa
    29821 aacactccgg cattcttatg acaagagtcg ccgcaaggga gcgattcatg tcattagtgc
    29881 gttctcaaca atgcacagtc tggtcatcgg acagatcagg acggatgaga aatccaatga
    29941 aattacacct atccctgaac ttcttaacat gctggatatt aaaggaaaaa tcatcacaac
    30001 tgatgcgatg ggttgccaga aagatattgc agagaagata caaaaacagg gcggtgatta
    30061 tttattcgct gtaaaaggaa atcaggggcg gcttaataaa gcctttgagg aaaaatttcc
    30121 actgaaagaa ttaaataatc cagaacatga cagttacgcc attagtgaaa agagtcacgg
    30181 cagagaagaa atccgtcttc atattgtttg cgatgtccct gatgaactta ttgatttcac
    30241 gttcgaatgg aaaggactga agaaattatg cgtggcagtc tcctttcggt ccataatagc
    30301 agaacaaaag aaagagccag aaatgacggt cagatattat atcagttctg ctgatttaac
    30361 cgcagagaag ttcgccacag caatccgaaa ccactggcac gtggagaata agctgcactg
    30421 gcgtctggac gtggtaatga atgaagacga ctgcaaaata agaagaggaa acgccgcaga
    30481 attattttca gggatacggc acatcgctat taatatttta acgaatgata aggtattcaa
    30541 ggcagggtta agacgtaaga tgcgaaaagc agccatggac agaaactatc tcgcgtcagt
    30601 ccttgcgggg agcgggcttt cgtaatcttg ccctggccga aatcgcgccc caaatggagg
    30661 ctttgataaa gaaacccggt tacagcatgt aatgcttaac ctgccgtaac tcgcttacca
    30721 ctggtaacaa acatgccagc acctaaatga tgcaacgtat tgagcttatc atcatcaaac
    30781 tgccagcggc cttcgacaaa tactcgtgcg tctgccgctg cagataccac ttcgccaaat
    30841 agcgtatcgt atttttcttg cgcggaagtc gcaggtagca ggcgacactc catccacgcc
    30901 agacattttt cctcgaccag cggcaaacca agcaccgggc ctctcactac cggaatgccg
    30961 tagcaattaa acttatcttc ttcacgaccc gacacacttc ccaccgccca cgtccagtta
    31021 gtcgccgcaa cgcccgggat aacgatgcca aatttaccgt taccttcaat caactccctg
    31081 gtccatgtcg atttatctac cacaatcgcc acgcgtggcg gttcaaactc tacgggcatc
    31141 gaccaggctg ctgccataat gttacgccgc tgggattgct catcgaaact ggtgatcatg
    31201 acagttgggc catgatttaa cagacagctg gcatggtgta attcgacggg aatgaattgg
    31261 ctcacgatat cgcctcgcta tgaagggtgt tttaaaagcg tagctcgtaa cgcaataagt
    31321 aacgaaatta acgggattgg cgatttgcga acgtgatgca tgtccgcgat cgcacaaaat
    31381 agccagtgcg gcgtctattc caggttataa gttgagaaaa ccactaaggg aaacgcctga
    31441 tgacgcccat tctgaatcat tattttgccc gtattaactg gttgggagct gctgcggcca
    31501 atattgatac gctgcgtgca ttgcacctga aacacaattg caccattccg tttgaaaatc
    31561 tcgacgtttt gctgccgagg gaaatacagc ttgatgatca atcgctggaa gagaaactgg
    31621 tgatagcccg tcgtggcggt tactgttttg agcagaatgg cgtgtttgag cgggtgttac
    31681 gcgagctggg gtttaacgtt cgcagcttgt taggtcgcgt agtgttatca aatccgccag
    31741 cattaccgcc gcgcacccat cgtttgctgt tggtggaact ggaaggggaa aaatggattg
    31801 ctgatgtcgg tttcggtggg cagacgctaa ccgcgccgat tcgtttaatg ccggatatca
    31861 tgcaaaccac gccacacgga gagtatcggt tgttgcaaga gggtgatgat tgggtattgc
    31921 agtttaatca tcatcagcac tggcagtcga tgtaccgttt tgatctctgc gagcagcaac
    31981 aaagcgatta tgtgataggc aatttctggt cggcgcactg gccgcagtcg cattttcgcc
    32041 atcatttgct gatgtgtcgc catttgccgg acggtggcaa gctaacgctg accaattttc
    32101 attttaccca ttatgaaaat gggcacgcag ttgagcagcg aaatctacca gatgtggcgt
    32161 cgttatatgc cgtgatgcag gaacagtttg gtctgggcgt agatgatgcg aaacatggct
    32221 ttactgtgga tgagttagcg ctggtgatgg cggcgtttga tacgcacccg gaggcgggaa
    32281 aataatttat gtcaggttgc cggatgtggc gtaaatgcct tatccggcct acattagtcc
    32341 ggttgtaggc ctgataagcg aagcgcatca ggcaacggca tcaaaaatca atggcccatt
    32401 cagcatggaa tagaatcacc gccgtgccgg aaatggtaac tttctccggt tggttatcgc
    32461 gaattgtcac cgtcacatca attacaccat cacgccccaa tgcgcgcccc tgatggcctt
    32521 taacacgcaa cacgttgcca tcgtggggca atacgttgtg atgtactaac catgcgccca
    32581 tcggaccatt ggcattcccg gtcaccggat cttccacaat accaatcgca ggcgagaaca
    32641 tgcgaccgtc ggtttcgctt ttgcctgggc ggatctggaa cgggaagaaa ccattgcagc
    32701 caatctgttt gctgatagcg gtcaacgcag caagatcagg cgaaagggcg tcgatatcca
    32761 cttctggttt caacgggatc atcacttttg agtgtcctgt tgttgccacc tgaattggca
    32821 agcctggcag aatatcgtcc tcagtgagat gaagcgcgtt gataatcgct gcacgtgttt
    32881 cacctgtcag tggtggctca aagcctggcg taccttgttc cagcgaaata cgataatcat
    32941 cattttgctt ttcgatagtc acacgatgtt ttcctgccag cgacgtttgc cagaccgtgc
    33001 aatttcccaa acctaatacc tttgaacgta catagtgcgc agccaccgtc gcgtgaccgc
    33061 aaattggcac ttcaaccgtt ggcgtaaagt agcggatgcg cacatcgctg tcgtcgctgt
    33121 gcagcagaaa agcagtttcc gaatgaccta actcgcgggc gataagctgc atttgcgctt
    33181 cactgagatt atcggcgggg aaaacaacac cggcagaatt gccgcgaaac ggttgagagg
    33241 taaaagcatc gacgtggtac acttgcggtt tcattaacgt tctcctgtgg ctggagaact
    33301 atcatagcct gcaagtggcc ggagagcgaa gggctatccg gcccgcgtga gaattaacgc
    33361 cgcgaacgca caatctgata ccgacgagta aagtactcaa acggcgcgct ccagacatgc
    33421 accagtcggg tgaacgggaa gagcaggaag atggtcattc caagcaccag atgaacgcgg
    33481 aatataaagg cgacaccatc aagatgcgta gatgcaccgc cctggaaagt gacaatgctt
    33541 tgcgcccagc ccaccagctt catcatttca ctgccgtccg gatgttgtgc tgaaaaagga
    33601 atggtggtta acccgagaat gcactgaatt aacaaaatgg cgaggatcag gatatccgca
    33661 ccagaagacg tcgccctgat acgcggattt gttaaacgac gcaccagtag ccccgcaccg
    33721 ccaatcagcg tcaatacccc gcatattccg ccgaggatca tcgccatctg ctgttttacc
    33781 gccaccggca gaaaccacgc gtacatccag tgtggcgtta acatgccgaa caggtgcccg
    33841 aagaaaatcc ccaaaatgcc gatatggaac aagtttgacc acagcaccat cccgcgttta
    33901 tccagcatct ggctggaggc ggcgcgccag gtgtactgcc cgtagtcgta gcgcagccag
    33961 cttccaagga taaaaaccgt gccacacaag tacgggtaga tatcgtaaaa aaagacgttc
    34021 agatactgaa tcatttccca cctcccgcac tgatgtcgac atactgcggc gcgacatcct
    34081 ggctaaagcg tcgctgatat tgattaagcg gcgagctgtc acacgccgtg gcgttatctt
    34141 caataaactt cacctgttct tcttcccaca ccgcatccag cgcctggcgg gtgtcgtcac
    34201 gctcttcact gcgaatctgt tttgtgacac tgtcacttga cagagggctt cctgccagtt
    34261 gcaacagggc gtcaaataac gcgtaccacg gtgcctcgcg ttgttttaag cgtccgccaa
    34321 gcagggtgag gatcggcgca acgttgagta atccttcttt cgcctgatcg tccggaagta
    34381 cgcttaaata ctccagatac agcggtagat aatcgggcag ttccctacaa ttcagttgca
    34441 gaccgacctt ttcatactcc gccagcaggt ccaccattgc ctggccgcga tcgcgggact
    34501 cggcatgaac atgttcaaac agcagcagcg acgtggtgcg cccgcggtca aacacttcac
    34561 accattcggc ctgtttatcc agcagcgggg cgttgagcag gtcgagtgca aaatcggtga
    34621 gcatcggcgc gtcgcggcgg ataagcgcca gcgcgtcgtc cttacactcc cacaacaact
    34681 cgtctgggta ctccatcaac aggccgagca ctttgaggat ctgcattatt cgccctccgc
    34741 tttgtcgcgc acttcggtga tgttgatggc atcgatacgg ctactgttga acaggttgaa
    34801 tttgctgtcc gaaccgtggc aaccgtcgcc aaaggtaaaa ccgcagccgt tgcgttctgc
    34861 gaaggcatcg cccgccattt cccgatggct tgtcgggatg acgaaacgat cttcatagtt
    34921 ggcaatggcg aggtaacgat acatctcttc gacctgggcg acgctcagac ctacttcgtc
    34981 gatggcacga gtatcagtaa cgccttccac ggtttgtgaa cgcatatagt ggcgcatcgc
    35041 catcatccgt ttcagcgccc gcagtaccgg accggtatca ccggcactca acatattggc
    35101 gagatattgc accggaatac gcaggctttc gatggcgggc agcacgcctt cgcttttcgg
    35161 caaacctccc gcatcagcgt aggactgaat cggtgacagc ggaggaacgt accagaccat
    35221 cggcagggtg cggtattcag ggtgcagcgg tagcgccagt ttccagtcca tcgccatttt
    35281 gtagacaggc gaacgctggg cagcgtcaat cacgttttgt ggaatacctt gtttcagggc
    35341 ttcttcgatc actgagggat cgtgcggatc gaggaacact tcgcactggc gttcatagag
    35401 atcgacctct cgttcggtac tcgctgcttc ttcgatgcgg tcggcgtcgt agagcagcac
    35461 gcccagatac cggatgcgac ccacgcaggt ttcggagcag actgttggct gaccggactc
    35521 aattcgcgga taacagaaga tacatttttc tgacttgccg cttttccagt tgaagtagat
    35581 ttttttgtac ggacaaccgc taatgcacaa acgccagcca cggcatttat cctgatcaat
    35641 cagcacaatg ccgtcttctt cgcgtttgta gatagcgccg ctggggcagg tcgccacgca
    35701 gctgggattg aggcagtgtt cgcacaggcg cggcaggtac atcatgaagg tgttttcaaa
    35761 ctgcccgtac atctcctttt gcatggcctc gaagttgcgg tcgcgggctc gtttttcaaa
    35821 ttcgccgccc agcagttctt cccagtttgg gccccagatc actttgtcca tccgtttgcc
    35881 gtcaatcagt gaacgcggac gggcggtggg aatatgtttg ccttccggcg cgctatgcaa
    35941 atgttcgtag tcgaaggtga aaggttcgta gtaatcgtca atctgcggca ctaccgggtt
    36001 ggcgaaaatt ttggttatta cgcccatctt gctgcccaga cgcgggcgta tcttgccatt
    36061 cacatcacgc acccagccac cttgccactc atcctgatct tcccagtttt tcggataacc
    36121 aatgcccggt ttggtttcga cgttgttaaa ccatgcgtac tccatgcctt cgcgaccggt
    36181 ccagacgttt ttacaggtca ccgaacaggt atggcagccg atacatttat cgaggttaag
    36241 caccatgccg acttgcgaac gtattttcat tttttcgcct cctgaacctg atcgcgacct
    36301 tcatcatcca gccagttaac gttcttcatc ttgcggatca tgatgaattc gtcgcggtta
    36361 gagccgacag tgccgtagta gttaaagccc caggccagct gcgcgtaacc gccaatcata
    36421 tgtgttggtt tcggacaaac gcgggtgacc gagttatgaa tgccgccgcg catgccggtt
    36481 acttccgaac caggaatatt cataatgcgt tcctgagcgt gatacatcat ggtcatgccc
    36541 ggcggtacac gctggctgac caccgcgcgg gccgtcagcg cgccattggc gttaaacact
    36601 tccacccagt cgttatcgac aatggtcagt tcacgggcat cggtttcgct gatccagaca
    36661 atcggtccac cgcgagagag cgtcagcatt agcaggtttt cactgtaggt tgagtgaatg
    36721 ccccatttct ggtgcggtgt caggaagtta agggcttttt ccgggaagcc gtttggcggg
    36781 atttggcgca tttcactgac actgcgggtg tcgataggcg ggcgataagc caccaggctt
    36841 tcaccaaagg cacgcatcca cggatgatcc tgataaagct gttgacggcc cgacagtgtg
    36901 cgccacggaa ttagctcatg aacgttggtg tatcccgcat tataggagac gtgatcgctt
    36961 tccagaccag accatgtggg gctggagata attttacgcg gctgcgcctg aatatcgcga
    37021 aagcgaatct tctcgtcctc tttgtgcagc gccagatggg tatgttcgcg tccggtgatc
    37081 tcgcccagcg cttgccacgc tttgactgca acatggccgt tggtttccgg tgccagcgcc
    37141 agaatcactt ctgaggcatc aatcgcggta tcaatcagcg gacgcccctg tgccgggcca
    37201 tcacgcttgg tgtaattgag tttaccgagg aaatcaattt catcctgggt attccacgaa
    37261 atccctttac ctccgttgcc aagtttgtcc atcaatggcc cgagcgaggt aaagcgttca
    37321 taggtagcag ggtagtcgcg ctccaccgcc acaatattcg gcgcggtttt gcctggaatc
    37381 agatcgcact cgcctttgcg ccagtcgagc acttcacacg gctgtgaaag ctctgccggg
    37441 gagtcatgca gcagtggttg taataccacg tcggtttctt tgccgagatg ccccacgcac
    37501 acttgcgaaa aggctttagc gatacctttg tagatttccc agtcgctgcg tgattcccac
    37561 gccggatcga ccgccgcaga aagcggatga ataaacggat gcatatccga ggtattcata
    37621 tcgtcttttt cgtaccaggt ggcggtgggc agaacgatat cggagaacag gcaggtactg
    37681 gacatgcgga aatcgagcgt caccagcagg tcgagcttgc cttcaatcgc cgcagtttgc
    37741 cactcgactt cttctggttt gatcccgtcg ctggcaccga gttcctctcc ctgaatcccg
    37801 ctttcggtcc ccagcagata cttctgcata tactcgtggc ctttgccgga ggagccaagc
    37861 aggttagaac gccagacaaa caaattacgc ggatgattgc tgccgctatc tggttgttcg
    37921 caggccatac gtaaatcgcc cgatttcagc gcctgggcgg taaattctgt gggggataat
    37981 ccggccttgt cggcttcagc tttcagggag agcgggttac gccccagctg cggcgcagaa
    38041 gggagccagc ccatacgttc ggcgcgaacg ttgaaatcaa tcaggtgacc gctgtattta
    38101 ctggcatcgg cgagcggtga gagcaattcc tgcgcggtga ctttttcata gcgccactgg
    38161 ctggagtgat tgtagaaaaa cgaggtgctg ttcatctggc gcggtggacg gttccagtcg
    38221 aacgcaaagg ccagcggcaa ccagccggtt tgtgggcgca gtttttcctg accgacatag
    38281 tgcgcccagc cgccgccgct ttgcccgaca caaccgcaga agatcagcat attgatcatc
    38341 ccgcggtagt tcatgtccat gtgataccag tggttaacac ctgcgccgag aataatcatc
    38401 gagcgcccat gcgttttatg agcggtatcg gcaaattcac gggcgatggt ttcaatatac
    38461 tggcgcggta cgccggtaat ttgctcgccc caggcagggg tgtaaggttt gatttcagcg
    38521 taatctttcg cgctgttttc atcttccagc ccgcgatcga ggccgtaatt tgccagtacc
    38581 agatcataaa cgctgaccac cggacaggtg ctaccatcgg ctaatgtcag atttttaacg
    38641 ggcaattggc gcaccaggac aggattgtgt tttacgctgc gaaaatgcgg attttcaatg
    38701 cctccaaagt aggggaaggc cacgcctgca acggcgtcat gttgaccgag cagggttaac
    38761 gacaattcgg tttccgtacc ggcggcaatg gattccaggt tccatttgcc tttttctccc
    38821 caacgaaaac caatcgaacc gttcggcact accaattcac ctgcggtatt aactgccacg
    38881 gttttccact gcggattatt gctttcgccc agtccatcca ccaggtcaga tgcgcggacc
    38941 atgcgcccgg gaacgtagct gccgtcgtcg cgaggttcca gcattaccag catcggcatg
    39001 tcgctgtagc ggcggcagta gttgataaaa taatcgctgg gattatcgag atgaaactct
    39061 tttaaaatga catggcccat tgccattgcc agggcactat cagtgccttg tttcggtgcc
    39121 agccactggt cgcacaattt ggccacttca gagtagtcag gggtaatggc gatggtttta
    39181 gtgcctttgt agcgtacttc ggtaaagaaa tgggcgtccg gcgtacgtgt ctgcggtacg
    39241 ttagaccccc aggcgatgat gtaactggag ttataccagt cggcagattc cggtacgtcg
    39301 gtttgctcgc cccaggtcat cggcgaggcg ggcggcaggt cgcaatacca gtcgtagaag
    39361 cttaaacagg tgccacctaa cagcgacaga tagcgtgttc cggcagcgta agaaaccatc
    39421 gacatcgccg gaatcggcga gaaaccggca acgcgatccg ggccgtaagt tttgatggtc
    39481 cagacgttag cggcggcaat cagctgattt agctcctgcc agttggagcg gataaaacca
    39541 ccgcgtccac gcacttgttt gtagctcagg cacttttgcg gatcgttcat aatcgatgcc
    39601 cacgccagta ccggatcgct gtgttgcttg agggcttcgc gccacagttc aatcagtcgt
    39661 ttacgaatga gcgggtattt caggcggttc gcgctgtaga gataccagga gtaacttgcg
    39721 ccacgcgggc agccgcgagg ttcgtgattc ggcaggtcag ggcgagtgcg cgggtaatcg
    39781 gtctgttgga tttcccaggt caccagacca tttttaacgt agattttcca gctacaggag
    39841 cctgtacagt taacaccgtg ggtggaacgc acgattttgt cgaactgcca acgctggcga
    39901 tagctgtcct cccagtcgcg gttgctatgc atcacctgtc cgtgaccatc ggcaaagatt
    39961 tcgccctttt gtttgaagta gcgaaagcga tccaaaagtt tactcatgac ataactcctg
    40021 ctccaggaat gaattgggat ctgatgcctt gtcggatgcg acacggttgt agcctgataa
    40081 gacgtgtcag gcatcgatct cttatttttg gctgaacttc cgccgaccat aaaccagcca
    40141 ggtcagtagc acacaaacga tgtagaagat taaaaacact ttcatcgcgc ccaccggaga
    40201 gccggtcata ttgagcgaca tgccaaacgc ctgcggaata aaaaagccgc ccactgcgcc
    40261 aatggctgag ataaagccca gagcggctgc cgtttcggtg acggcttctc tttgagcttg
    40321 ctcatcacta ccgcctttca tcttcacccg ataaattgtt atctggcgaa agatgacggc
    40381 gatcatctgg aaagtagaac cacttcccag acccgcggtc agaaacagcc ccataaatac
    40441 ggcgtaaaat gcgatgaaat taccggagcc tgtgcccggt aaggtaagga acagcagggc
    40501 gctgaaaatc gccataaaaa tgaagttgat caacgtcacc cgcacgccgc cgaatttatc
    40561 ggaaatagca ccaccaaccg atcgcgcgat ggcaccgata aatgggccaa agaacgccag
    40621 gcgcagaata ttcacatccg ggaactgggt ttttgccagc atggcaaaac ccgcagaaaa
    40681 accgataaac gaaccgaagg tggcaaggta aagcaggctc agcagccaga gatgcaggcg
    40741 ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa ctggcgatat cattcatccc
    40801 tgaccaggcg gcgatcgtgg caatcgccag taatggcacc caaatccatg cggcattcgc
    40861 cagcgacatc accgaaccgt cggcctgcgg tacgccattg acgccgagaa aggcaaatac
    40921 ggggacaaaa atgaccagcg gtgcaaccag ctgcattaca cttacaccta agtttcctaa
    40981 tccgccatta atcccaagag cgctcccttg tttggctttt ggaaagaaga aactgatatt
    41041 gcccatgctc gaagcaaagt tcgcacctgc aaaaccgcat agcaaagcga taacgataaa
    41101 tatcccaaaa ggagtattcg gattctgcac ggcaattccg agccagacgc aaggaataat
    41161 caggattgca gtactaaaaa ccgtccatcg gcgtccaccg aatataggca ccataaagga
    41221 gtaggggaca cgcaataatg cgccagaaac ggagggtaat gcggttaata aaaagagttg
    41281 atcggtagta aaattaaaac cgattttatt gagattaaca gtaactgcgc taaatagcat
    41341 ccagacacag aaggcaagaa gtagacaact gactgatatc cagaggtttc ttcgtgcaat
    41401 atgctttcct ttattttccc agaaggccgg attttctggt ttccagtcgc gtaaaagata
    41461 acgactattt ttctcatttt gcagtgccat attgttcctc acatgcacac attggtaatg
    41521 aaaaaaagac aaaacaggag gtaaggcgca atagccagtt attagaatta aggatgaatc
    41581 aggtgaagtg ctgattagaa gaatagataa gaaagcgtaa ctgcgggggc agaatgtgga
    41641 ttaaacagac agatatgtgt tactaaatgt aactaaaatg actttctggt atttgaaatt
    41701 tattttttaa tattgtcaga atttatctga ttaactgccg ggccgtagac ccgacaatta
    41761 ttttagccac gacgtgtcgc cagccagcag agcagggaac cgccgcagac cattagcgcg
    41821 ccttgccaga acgagaacga cagcggggcg ctgagcagca cggctgcaag cgctgaggaa
    41881 aggacgggcg taaaatacga acctaccgcc attatggtga cgttgccatg taatataccg
    41941 acattccatg cagcataagc aaatcctaag gtaaatgccg cagagatgag tttaatcatg
    42001 acgggcgtgc taaataccat ttctggttgt ggcgtaagaa aatagtaaac ccacagactt
    42061 gctcccgtta gcaggacaaa aacggtaatt ccgttaaatc cgcgtgcgta tttattcgtt
    42121 actgtgcagt agactgccca gataaacgcc ccaatgaacg ccaggaaata actcaatggg
    42181 ctggtggtga tattattgat aatttcatca taatgtaacc cattgtcacc gcctaacacc
    42241 cagcagacgc cgaccagggc taataataat cctggtacaa tcagccagtt ggttttctga
    42301 ccattaaaca gaatggcaaa gagaattgtc aggctgggcc atagatagtt caccatcccc
    42361 acttcaatcg cctgatgacg ggtcgccgca taccctaagg aaagcgccag acagatttca
    42421 tagctgacga ataacagact cccggcgagt aaatagcctt tcgggatctg ccgaatacgc
    42481 ggaaatccaa ccgtgaagat taacagcagc ccgcttaatg aatagatagc agctgcgccg
    42541 ccgaccgggc cgagtccctc actgacaccg cgaatcaatc ctaccatcgt gctccacagg
    42601 acgatcgcta ttagccctat gagcgttgct ttttgacgtg tcatgctcgc tgttttgtct
    42661 ctcttgccgt taaaaattaa gctgaatttt atagcatttt tttaactggc ctgtcaggca
    42721 gtggtgcgtt tttctaccgc tattgaggta ggtcaatttg cgcaggcgga ttattttgtg
    42781 gcaaacacgt gttctttttg atttcgcgca aaaagattca gaattttact gttagtttcc
    42841 tcgcgcagta atacccctga aaaaagagga aagcaatgga cgtcagtcgc agacaatttt
    42901 ttaaaatctg cgcgggcggt atggcgggaa caacagtagc agcattgggc tttgccccga
    42961 agcaagcact ggctcaggcg cgaaactaca aattattacg cgcgaaagag atccgtaaca
    43021 cctgcacata ctgttccgta ggttgcgggc tattgatgta tagcctgggt gatggcgcga
    43081 aaaacgccag agaagcgatt tatcacattg aaggtgaccc ggatcatccg gtaagtcgtg
    43141 gtgcgctgtg cccgaaaggg gccggtttgc tggattatgt caacagcgaa aaccgtctgc
    43201 gctacccgga atatcgtgcg ccaggttctg acaaatggca gcgcattagc tgggaagaag
    43261 cattctcccg tattgcgaag ctgatgaaag ccgaccgtga cgctaacttt attgaaaaga
    43321 acgagcaggg cgtaacggta aaccgttggc tttctaccgg tatgctgtgt gcttccggtg
    43381 ccagcaacga aaccgggatg ctgactcaaa aatttgcccg ctccctcggg atgctggcgg
    43441 tagacaacca ggcgcgcgtc tgacacggac caacggtagc aagtcttgct ccaacatttg
    43501 gtcgcggtgc gatgaccaac cactgggtgg atatcaaaaa cgctaacgtc gtaatggtga
    43561 tgggcggtaa cgctgctgaa gcgcatcccg tcggtttccg ctgggcgatg gaagcgaaaa
    43621 acaacaacga tgcaaccttg atcgttgtcg atccccgttt tacgcgtacc gcttctgtgg
    43681 cggatattta cgcacctatt cgttccggta cggacattac gttcctgtct ggcgttttgc
    43741 gctacctgat cgaaaacaac aaaatcaacg ccgaatacgt taagcattac accaacgcca
    43801 gcctgctggt gcgtgatgat tttgctttcg aagacggtct gttcagcggc tacgacgctg
    43861 aaaaacgtca atacgataag tcgtcctgga actatcagtt cgatgaaaac ggctatgcga
    43921 aacgcgatga cacgctgact catccgcgct gtgtatggaa tctgctgaaa gcgcacgttt
    43981 cccgctacac gccggacgta gtagaaaaca tttgcggtac gccaaaagcc gacttcctga
    44041 aagtgtgtga agtgctggcc tccaccagcg caccggatcg cacaaccacc ttcctgtacg
    44101 cgctgggctg gacgcagcac accgtgggtg cgcagaacat ccgtacgatg gcgatgatcc
    44161 agttgctgct cggtaacatg ggtatggccg gtggcggcgt gaacgcattg cgtggtcact
    44221 ccaacattca gggcctgact gacttaggcc tgctctctac cagcctgcca ggttatctga
    44281 cgctgccgtc agaaaaacag gttgatttgc agtcgtatct ggaagcgaac acgccgaaag
    44341 cgacgctggc tgatcaggtg aactactgga gcaactatcc gaagtttttc gttagcctga
    44401 tgaaatcttt ctacggcgat gccgcgcaga aagagaacaa ctggggctac gactggctgc
    44461 cgaagtggga ccagacctac gacgtcatca agtatttcaa catgatggat gaaggtaaag
    44521 tcaccggtta tttctgccag ggctttaacc cggttgcgtc cttcccggac aaaaacaaag
    44581 tggtgagctg cctgagcaag ctgaagtaca tggtggttat cgatccgctg gtgactgaaa
    44641 cctctacctt ctggcagaac cacggcgagt cgaacgatgt cgatccggca tctattcaga
    44701 ctgaagtatt ccgtctgcct tcgacctgct ttgctgaaga agatggttct atcgctaact
    44761 ccggtcgctg gttgcagtgg cactggaaag gtcaggacgc gccgggcgaa gcacgtaacg
    44821 acggcgaaat tctggcgggt atctaccatc atctgcgcga gctgtaccag gccgaaggtg
    44881 gtaaaggcgt agaaccgctg atgaagatga gctggaacta caagcagccg cacgaaccgc
    44941 agtctgatga agtggcgaaa gagaataacg gctatgcgct ggaagatctc tatgacgcta
    45001 atggcgtgct gattgcgaag aaaggtcagt tgctgagtag ctttgcacat ctgcgtgatg
    45061 acggtacaac cgcatcttct tgctggatct acaccggtag ctggaccgag cagggcaacc
    45121 agatggctaa ccgcgataac tccgacccgt ccggtctggg gaatacgctg ggatgggcct
    45181 gggcgtggcc gctcaaccgt cgcgtactct acaaccgtgc ttcggcggat atcaacggta
    45241 aaccgtggga tccgaaacgg atgctgatcc agtggaacgg tagcaagtgg acgggtaacg
    45301 atattccgga cttcggcaat gccgcgccgg gtacgccaac cgggccgttt atcatgcaac
    45361 cggaagggat gggacgcctg tttgccatca acaaaatggc ggaaggtccg ttcccggaac
    45421 actacgagcc aattgaaacg ccgctgggca ctaacccgtt gcatccgaac gtggtgtcta
    45481 acccggttgt tcgtctgtat gaacaagacg cgctgcggat gggtaaaaaa gagcagttcc
    45541 cgtatgtggg tacgacctat cgtctgacgg agcacttcca cacctggacc aagcacgcat
    45601 tgctcaacgc aattgctcag ccggaacagt ttgtggaaat cagcgaaacg ctggcggcgg
    45661 cgaaaggcat tgccaatggc gatcgtgtca ctgtctccag caagcgtggc tttatccgcg
    45721 cggtggctgt ggtaacgcgc cgtctgaaac cgctgaatgt aaacggtcag caggttgaaa
    45781 cggtgggtat tccaatccac tggggctttg aaggtgtcgc gcgtaaaggt tatatcgcta
    45841 acactctgac gccgaatgtc ggtgatgcaa actcgcaaac gccggaatat aaagcgttct
    45901 tagtaaacat cgagaaggcg taagggggcg aacagatggc tatggaaacg caggacatta
    45961 tcaaaaggtc cgcaactaac tccatcacgc cgccttctca ggtgcgtgat tacaaagcag
    46021 aagtcgcgaa acttatcgac gtttccactt gtatcggctg taaagcctgt caggtggcgt
    46081 gttcggagtg gaacgacatc cgtgatgaag tggggcactg cgtcggggtt tacgataacc
    46141 ccgccgatct gagcgccaag tcctggacgg tgatgcgctt tagcgaaacc gaacagaacg
    46201 gcaagctgga gtggctgatc cgtaaagacg gctgtatgca ctgtgaagat ccgggctgcc
    46261 tgaaggcatg cccgtctgct ggtgcaatca ttcagtacgc taacgggatt gtcgatttcc
    46321 agtcggaaaa ctgcatcggc tgtggttact gcattgccgg gtgtccgttt aatattccgc
    46381 gcctcaacaa agaggataac cgggtatata aatgcacgct ctgtgtcgat cgcgtcagcg
    46441 tcggccagga accggcttgt gtgaaaacct gtccgaccgg ggctatccac ttcggcacca
    46501 agaaggagat gctggagctg gcggaacagc gcgtggcgaa actgaaagcg cgtggttacg
    46561 aacatgctgg cgtctacaac ccggaagggg tcggtggtac gcacgttatg tacgtgctgc
    46621 atcacgccga tcagccggag ctgtatcatg gtctgccgaa agatccgaag atcgatacct
    46681 cggtaagcct gtggaaaggc gcgttgaaac cgctggcagc ggctggcttt attgccactt
    46741 ttgccgggtt gattttccac tacatcggta ttggcccgaa taaggaagtg gacgatgagg
    46801 aggagggaaa tcatgagtaa gtcgaaaatg attgtgcgca ccaaatttat tgatcgcgcc
    46861 tgtcactgga ccgtggtgat ttgcttcttc ctggtggcgc tgtccgggat ttcgttcttc
    46921 ttcccgacgc tgcaatggct gacgcaaacc ttcggtacgc cgcagatggg acgcattttg
    46981 cacccgttct tcggcattgc gattttcgtc gcgctgatgt ttatgtttgt gcgttttgtg
    47041 catcacaaca tcccggataa gaaagatatt ccgtggctgt tgaacattgt cgaagtatta
    47101 aaaggcaatg agcacaaagt ggcggatgtc ggtaagtaca acgccgggca aaagatgatg
    47161 ttctggtcaa tcatgagcat gattttcgtg ctgctggtga ccggagtgat tatctggcgt
    47221 ccgtactttg cgcagtactt cccgatacag gttgtccgtt atagcctgct gatccatgcg
    47281 gctgcgggta tcatcctgat ccacgccatc ctgatccata tgtatatggc attctgggtg
    47341 aaaggatcga ttaaagggat gatcgaaggg aaggtgagcc gtcgctgggc gaagaaacac
    47401 catccgcgct ggtatcgtga aatcgagaag gcagaagcga aaaaagagag tgaagaaggg
    47461 atataatctc ttttgaactt taagctgaaa
//
LOCUS       DAOFSV010000022        47311 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.022, whole genome shotgun sequence.
ACCESSION   DAOFSV010000022 DAOFSV010000000
VERSION     DAOFSV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.022"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            38..1159
                     /locus_tag="QBH27_001337"
     CDS             38..1159
                     /locus_tag="QBH27_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDQ7013670.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            1159..4266
                     /locus_tag="QBH27_001338"
     CDS             1159..4266
                     /locus_tag="QBH27_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDQ7013671.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            4270..5643
                     /locus_tag="QBH27_001339"
     CDS             4270..5643
                     /locus_tag="QBH27_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDQ7013672.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            5652..6815
                     /locus_tag="QBH27_001340"
     CDS             5652..6815
                     /locus_tag="QBH27_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDQ7013673.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFIHETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGTGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(6863..7669)
                     /gene="sapF"
                     /locus_tag="QBH27_001341"
     CDS             complement(6863..7669)
                     /gene="sapF"
                     /locus_tag="QBH27_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDQ7013674.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(7671..8663)
                     /gene="sapD"
                     /locus_tag="QBH27_001342"
     CDS             complement(7671..8663)
                     /gene="sapD"
                     /locus_tag="QBH27_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDQ7013675.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(8663..9553)
                     /gene="sapC"
                     /locus_tag="QBH27_001343"
     CDS             complement(8663..9553)
                     /gene="sapC"
                     /locus_tag="QBH27_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDQ7013676.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(9540..10505)
                     /gene="sapB"
                     /locus_tag="QBH27_001344"
     CDS             complement(9540..10505)
                     /gene="sapB"
                     /locus_tag="QBH27_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDQ7013677.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINPI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(10502..12145)
                     /gene="sapA"
                     /locus_tag="QBH27_001345"
     CDS             complement(10502..12145)
                     /gene="sapA"
                     /locus_tag="QBH27_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDQ7013678.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(12458..12703)
                     /locus_tag="QBH27_001346"
     CDS             complement(12458..12703)
                     /locus_tag="QBH27_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDQ7013679.1"
                     /translation="MNHDIPLKYYDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(12837..12974)
                     /locus_tag="QBH27_001347"
                     /pseudo
     CDS             complement(12837..12974)
                     /locus_tag="QBH27_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            complement(13128..14105)
                     /gene="pspF"
                     /locus_tag="QBH27_001348"
     CDS             complement(13128..14105)
                     /gene="pspF"
                     /locus_tag="QBH27_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDQ7013680.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQL"
     gene            14272..14940
                     /gene="pspA"
                     /locus_tag="QBH27_001349"
     CDS             14272..14940
                     /gene="pspA"
                     /locus_tag="QBH27_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDQ7013681.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            14994..15218
                     /gene="pspB"
                     /locus_tag="QBH27_001350"
     CDS             14994..15218
                     /gene="pspB"
                     /locus_tag="QBH27_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDQ7013682.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            15218..15577
                     /gene="pspC"
                     /locus_tag="QBH27_001351"
     CDS             15218..15577
                     /gene="pspC"
                     /locus_tag="QBH27_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDQ7013683.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            15586..15807
                     /gene="pspD"
                     /locus_tag="QBH27_001352"
     CDS             15586..15807
                     /gene="pspD"
                     /locus_tag="QBH27_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDQ7013684.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            15882..16196
                     /gene="pspE"
                     /locus_tag="QBH27_001353"
     CDS             15882..16196
                     /gene="pspE"
                     /locus_tag="QBH27_001353"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDQ7013685.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            16407..18086
                     /gene="ycjM"
                     /locus_tag="QBH27_001354"
     CDS             16407..18086
                     /gene="ycjM"
                     /locus_tag="QBH27_001354"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDQ7013686.1"
                     /translation="MQQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFITVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELDNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            18100..19392
                     /locus_tag="QBH27_001355"
     CDS             18100..19392
                     /locus_tag="QBH27_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7013687.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQR"
     gene            19413..20294
                     /locus_tag="QBH27_001356"
     CDS             19413..20294
                     /locus_tag="QBH27_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDQ7013688.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            20281..21123
                     /locus_tag="QBH27_001357"
     CDS             20281..21123
                     /locus_tag="QBH27_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDQ7013689.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            21154..22206
                     /locus_tag="QBH27_001358"
     CDS             21154..22206
                     /locus_tag="QBH27_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDQ7013690.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFSPRPANAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            22224..23012
                     /locus_tag="QBH27_001359"
     CDS             22224..23012
                     /locus_tag="QBH27_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDQ7013691.1"
                     /translation="MKIGTQNQAFFPGNILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDHRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEAPAQAYRDSLAWLRTC"
     gene            23022..24077
                     /locus_tag="QBH27_001360"
     CDS             23022..24077
                     /locus_tag="QBH27_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ7013692.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASLWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFAFDTQQLREQVTNGVLGEIYVTTARA
                     LRRSGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            24074..26341
                     /gene="ycjT"
                     /locus_tag="QBH27_001361"
     CDS             24074..26341
                     /gene="ycjT"
                     /locus_tag="QBH27_001361"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDQ7013693.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHIDEIQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGRDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNERLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            26338..26997
                     /gene="pgmB"
                     /locus_tag="QBH27_001362"
     CDS             26338..26997
                     /gene="pgmB"
                     /locus_tag="QBH27_001362"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDQ7013694.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            27011..28093
                     /locus_tag="QBH27_001363"
     CDS             27011..28093
                     /locus_tag="QBH27_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013695.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGELLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     IQKIAKEMIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            28138..29043
                     /gene="ompG"
                     /locus_tag="QBH27_001364"
     CDS             28138..29043
                     /gene="ompG"
                     /locus_tag="QBH27_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDQ7013696.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNNWHFNIGAMYEIENVEGYV
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSLGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(29094..30092)
                     /locus_tag="QBH27_001365"
     CDS             complement(29094..30092)
                     /locus_tag="QBH27_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ7013697.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVQVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            30248..31645
                     /locus_tag="QBH27_001366"
     CDS             30248..31645
                     /locus_tag="QBH27_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDQ7013698.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGMIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            31642..32703
                     /locus_tag="QBH27_001367"
     CDS             31642..32703
                     /locus_tag="QBH27_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDQ7013699.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            32851..34392
                     /gene="tyrR"
                     /locus_tag="QBH27_001368"
     CDS             32851..34392
                     /gene="tyrR"
                     /locus_tag="QBH27_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDQ7013700.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(34436..34942)
                     /gene="tpx"
                     /locus_tag="QBH27_001369"
     CDS             complement(34436..34942)
                     /gene="tpx"
                     /locus_tag="QBH27_001369"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDQ7013701.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            35061..36026
                     /gene="ycjG"
                     /locus_tag="QBH27_001370"
     CDS             35061..36026
                     /gene="ycjG"
                     /locus_tag="QBH27_001370"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDQ7013702.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(36001..36729)
                     /gene="mpaA"
                     /locus_tag="QBH27_001371"
     CDS             complement(36001..36729)
                     /gene="mpaA"
                     /locus_tag="QBH27_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDQ7013703.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(37020..37658)
                     /locus_tag="QBH27_001372"
     CDS             complement(37020..37658)
                     /locus_tag="QBH27_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7013704.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(37729..38649)
                     /locus_tag="QBH27_001373"
     CDS             complement(37729..38649)
                     /locus_tag="QBH27_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7013705.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSLAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEVISVLAPFFEETL"
     gene            38787..39686
                     /gene="pgrR"
                     /locus_tag="QBH27_001374"
     CDS             38787..39686
                     /gene="pgrR"
                     /locus_tag="QBH27_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDQ7013706.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            40023..41636
                     /gene="mppA"
                     /locus_tag="QBH27_001375"
     CDS             40023..41636
                     /gene="mppA"
                     /locus_tag="QBH27_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDQ7013707.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(41687..42718)
                     /gene="ynaI"
                     /locus_tag="QBH27_001376"
     CDS             complement(41687..42718)
                     /gene="ynaI"
                     /locus_tag="QBH27_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDQ7013708.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPADQGR"
     gene            42962..43219
                     /locus_tag="QBH27_001377"
     CDS             42962..43219
                     /locus_tag="QBH27_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDQ7013709.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(43269..44219)
                     /gene="uspE"
                     /locus_tag="QBH27_001378"
     CDS             complement(43269..44219)
                     /gene="uspE"
                     /locus_tag="QBH27_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDQ7013710.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(44371..45123)
                     /gene="fnr"
                     /locus_tag="QBH27_001379"
     CDS             complement(44371..45123)
                     /gene="fnr"
                     /locus_tag="QBH27_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDQ7013711.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(45318..45833)
                     /gene="ogt"
                     /locus_tag="QBH27_001380"
     CDS             complement(45318..45833)
                     /gene="ogt"
                     /locus_tag="QBH27_001380"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDQ7013712.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(45844..45987)
                     /locus_tag="QBH27_001381"
                     /pseudo
     CDS             complement(45844..45987)
                     /locus_tag="QBH27_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(46376..47191)
                     /locus_tag="QBH27_001382"
     CDS             complement(46376..47191)
                     /locus_tag="QBH27_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7013713.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT        11729 a        11392 c        12430 g        11760 t
ORIGIN      
        1 ttcaatgagg agggtatttc tcaccctgga gaaaataatg aagtatatag caacatctgt
       61 agtggcaatg ctgctcttat cgggttgtga taatacgcaa agtaacaatt catccccgtc
      121 agaaacagaa gtcggcgttg ttacgctcaa atctcaaccg gtttcggtag tcagtgaatt
      181 aaccggacgc accagtgctg cgctcagtgc tgaagtacgt ccgcaggttg ggggaattat
      241 ccagaaacgc ttatttaagg aaggtgatct ggtcaaggct ggacagcctc tctaccagat
      301 tgatgcggcc agttatcagg ctgcatggaa tgaagcccgg gcagcattac aacaagcaca
      361 ggcactggta aaagccgatt gccagaaagc gcagcgttat gcccgactgg tgaaagagaa
      421 tggtgtttca caacaggatg ctgatgatgc tcagtctacc tgtgcacaag ataaagccag
      481 tgtagaggcg aaaaaagccg cactggaaac tgcgcgcatt aatcttgact ggaccacggt
      541 aaccgcaccg atttcggggc gtattggtat ttcgtcggta acccctggcg cactggtgac
      601 cgcgtcgcag gatacagcgt taacgactat tcgtggtctg gatacaatgt atgtcgacct
      661 cactcgctcc agtgtcgatt tattacgtct gcgtaaacag tcactggcga ccaacagtga
      721 caccatgagc gtctcactta ttctggaaga tggcacaacc tacagcgaaa aagggcgtct
      781 ggaactcacc gaagtcgcgg tggatgagtc taccggttcg gtgacattac gggcaatttt
      841 ccccaatcca caacagcagt tattaccggg aatgtttgtt cgcgctcgtg tcgatgaagg
      901 cgtgatggaa aacgctattc tcgcgcctca acagggcgtt acgcgcgatg ctaaaggcaa
      961 tgcaactgcg ctggtggtga ataaagacaa taaagtagag cagcgaacgc tcgaaacggg
     1021 agaaacgtat ggtgataaat ggctggtgct gaacggcctg cacaacggcg accgactgat
     1081 tgttgaaggt tctgccaaag tcacttcagg gcagaccgtc aaggctgttg aagttcaggc
     1141 taatggaggc aacgcctgat gttttcgcgc ttttttgttc gccgcccggt ctttgcctgg
     1201 gttatcgcca ttttgattat gttggcaggg attctggcta ttcgcacatt gcccgtcgcg
     1261 caatatcctg acgttgcacc gccgaccatt aaaatttcag ccacttatac tggtgcttct
     1321 gccgaaacgc tggagaacag cgtgactcag gttatcgagc aacaactcac tgggcttgat
     1381 aatttactct atttcagctc aaccagtagc tctgatggtt cggtcagtat taatgtgacc
     1441 tttgaacaag gtaccgatcc agatactgca caggttcagg tgcagaataa aattcagcag
     1501 gcggagtcgc gcctacccag cgaagtgcag caaacgggtg ttacggtgga gaaatcacaa
     1561 agcaactttt tgctgattgc tgccgtgtat gacaccactg acaaagcctc cagttcggat
     1621 atcgccgact ggctggtcag taacgttcag gacccgctgg cgcgtgttga aggtgtgggg
     1681 agtctgcaag tctttggcgc ggaatacgct atgcgcatct ggcttgaccc ggccaaactg
     1741 gcgtcttact cgctgatgcc ttcagacgtg caaagtgcta ttgaagcgca aaacgtgcag
     1801 gttactgccg ggaaaatagg ggcattgcct tcaccggata ctcagcaact gaccgcaacg
     1861 gtacgtgcgc agtctcgctt gcagacggtg gatcagttca aaaatattat cgtgaaaagc
     1921 cagtcagacg gcgcagttgt tcgtataaaa gatgtggctc gcgttgagat gggcagtgaa
     1981 gattataccg ctatcggcaa acttaacggt cacccgtctg ccggggttgc tgtaatgctt
     2041 tcgcccggtg cgaatgcgct gaatacggcg acgctggtca aggataagat tgccgaattc
     2101 cagcggaaca tgccgcaggg atacgacatc gcatacccga aagacagcac tgaatttatt
     2161 aaaatctccg tagaggacgt aattcagacg ctgtttgaag ccatcgtatt ggtggtttgc
     2221 gtgatgtatt tattcctgca aaacctgcgt gccacactga ttccggcgtt agctgtcccc
     2281 gtcgttttgc tgggcacatt cggcgttctt gcgttgtttg gctattcgat taataccctg
     2341 acgttgtttg caatggtgct ggcgatcggc ttgctggtgg acgatgccat tgtggtggta
     2401 gagaacgtcg aacgcattat gcgtgacgaa gggttgcccg cgcgtgaagc cacggaaaaa
     2461 tcaatgggcg agatttctgg cgcactggtt gccattgcgc tggtgttgtc agccgtattc
     2521 ctgccgatgg ccttctttgg gggatctacg ggggtaattt atcgtcagtt ctccatcacc
     2581 atcatctccg caatgctgct ttccgtggtg gtggcattga ccttgactcc cgccctgtgc
     2641 ggttccgtcc tccagcatgt tccaccacat aaaaaaggct ttttcggcgc atttaaccgc
     2701 ttttaccgcc gtactgaaga taaatatcag cgaggcgtaa tttatgtcct gcgccgtgca
     2761 gcccgaacga tggggcttta tgtcgtgctg ggtggtggaa tggccctgat gatgtggaaa
     2821 ctgccgggca gtttcttacc cactgaagac cagggcgaaa tcatggtgca gtacacgctg
     2881 ccggccgggg cgaccgctgc ccgtacagca gaagtgaatc gccagattgt tgactggttc
     2941 ctgattaacg agaaagcaaa taccgatgtc atctttaccg ttgatggttt cagttttagc
     3001 ggtagcggac agaacaccgg gatggcgttt gtttcgttga aaaactggtc tcaacgtaaa
     3061 ggggcagaaa acaccgccca ggctatcgcc ctacgggcaa ccaaagagct gggcacaatt
     3121 cgtgatgcca cggtatttgc gatgacgccg ccagccgttg atgggctggg ccaaagcaat
     3181 ggttttacgt ttgaattgtt agctaacggt ggaaccgatc gtgaaacact gctgcaaatg
     3241 cgtaatcagt tgatagaaaa agcgaatcaa agtccggagt tacattctgt acgcgccaat
     3301 gatttgccac aaatgccgca attgcaggta gatattgata gtaataaagc ggtgtcatta
     3361 gggttgagtt tgaatgatgt caccgacacc ctgtccagtg cgtggggcgg tacttatgtg
     3421 aatgacttta ttgaccgtgg tcgtgtgaaa aaagtctaca ttcagggcga cagcgaattt
     3481 cgttccgcgc cgtcagactt aggtaaatgg tttgtgcgcg gcagcgataa cgccatgaca
     3541 ccattctctg cttttgcgac cacccgctgg ctgtatggac cggaaagact ggtgcgctac
     3601 aacggctcgg cagcctatga aattcagggc gaaaacgcga ctggctttag ttccggcgat
     3661 gcaatgacaa aaatggagga actggcaaac agtcttcctg cgggaacaac ctgggcctgg
     3721 agtggtttgt cattgcagga gaaactggcc agcggtcagg cattaagtct gtatgcagtt
     3781 tctattctgg tggttttcct ctgccttgca gcactgtacg aaagctggtc agtcccgttc
     3841 tcggtcattc tggtgatccc tctggggctt cttggcgcgg cgctggcggc ctggatgcgt
     3901 gatttaaaca acgacgttta cttccaggta gcgctattaa ctactatcgg cttgtcgtcg
     3961 aaaaacgcca ttctgattgt ggaatttgct gaagcggcgg ttgcagaggg ctattccttg
     4021 agtcgtgcgg cattacgcgc ggcgcagact cgtttacgcc caatcatcat gacctcgctg
     4081 gcgtttattg cgggggtaat gccgctggcg atagcaaccg gcgcaggggc gaacagccgc
     4141 atcgccattg gtacgggcat tattggcggc acgctgaccg ctacattact ggccattttc
     4201 ttcgttcctc tgttttttgt actggtgaaa cgtttgtttg ccggtaaacc gcgccgtcag
     4261 gagtaagtta tgttgcgtcg ttctcttatt tttctggtgt tgttgagtgc gggatgtgtc
     4321 tcgctcgatc ctcactacag tacgcctgaa tccccaatcc cggcgacctt gccgggagca
     4381 cagggccagg ggaaggcaat cagccatgac tggcagcagg tgatccacga tccccgattg
     4441 cagcaggtag taaccattgc actgaacagt aaccgcgatg tgcaaaaagc gattgctgat
     4501 attgactcag cacgggcgct ctatgggcaa actaatgcgt cgttatttcc gacggtgaat
     4561 gcggccttaa gttcgacccg cagccgttcg ctggcgaatg gtacgggaac aactgccgag
     4621 gctgacggca cggtgtccag ttatacgtta gatctgtttg gtcgtaatca gagtttatct
     4681 cgggcggcgc gggaaacctg gcttgccagt gaatttacag cccagaacac tcgtttaacg
     4741 ctgattgctg aaatcagcac cgcctggctg acactggcgg ctgataacag caacctggcc
     4801 ctggcaaaag aaacgatggc cagtgcagaa aactcattga aaattatcca gcgccagcaa
     4861 caggttggca cggcggcagc gacagatgtc agtgaggcga tgagtgttta tcagcaagcg
     4921 cgcgccagcg ttgccagcta tcagacacaa gtgatgcagg ataaaaatgc gctgaactta
     4981 ctggcaggta caacgcttgc ggaaaatctg ctaccgggaa cactggaaag cctgccggaa
     5041 caaatgataa gtctggttcc tgcgggagta tcatctgatg ttctgctgcg tcgcccggat
     5101 atccaggaag cggaacataa tttgaagagc gccaatgcag atattggcgc ggcgcgggct
     5161 aactttttcc cgacgatttc gctgactgcc agtgccggtg ttggcagtga cgcgttgtcg
     5221 tcgttgttca gccacggaat gcagatctgg tcatttgcgc catcggtcac attaccgctg
     5281 tttactggcg gaagtaatct ggcgcagctt cgttacgcag aagcacaaaa gcgtggactc
     5341 attgccacct atgaaaaaac cgttcagagc gcatttaaag atgtcgctaa cgcactggcg
     5401 cggcggacca cgcttgaaga gcaactggat gcacagcgcc agtatgttaa agctgaacaa
     5461 caaacggtcg atgttggctt gcgtcgctat caggcgggtg ttggtgatta cctgaccgtg
     5521 ttgacggcgc agcgcagctt gtggagcgca cagcaggagc tgctggcact gcaactgact
     5581 gattttacaa accgaatcac gctctggcag tcgctgggcg gcggtatgtc atcactcaag
     5641 taaggaataa tatggctaga gtctctcttt catgggcatt gattcttggt cttttagccg
     5701 gtatcggccc gatgtgtacc gatctttatt tgccggcttt gccggagatg tctgagcaac
     5761 tggcggcaac cacgaccata acgcaattaa ccctgaccgc atcactgatt ggtcttggcg
     5821 tcggacaact gttatttggc cctctgagcg acaaaatagg gcgtaaacgt ccgttaatct
     5881 tgtcgttgct attgtttatt gtttcttcca ttttatgcgc gacaacgaac aatatttact
     5941 ggctggtggt ctggcgtttt attcaaggga tcgcgggggc gggtggttcg gtgctctctc
     6001 gttctattgc tcgtgacaaa tatcagggag taacgttgac ccagtttttt gcgctgttaa
     6061 tgacggtgaa tggcctggca ccggtgttgt cgccagtgct gggcgggtac attgttagca
     6121 cttttgactg gcgcacttta ttctgggtaa tggctgaaat tagcaccgta ctgttgctgg
     6181 gctgtctgtt atttattcat gagaccttgc cagaaaataa aaggggctca tcattgctat
     6241 taaccggacg aagcgtggtg cagaaccgcc gttttatgcg cttttgcctg attcaaagtt
     6301 ttatgctggc cggtttgttt gcatatatcg gctcttcgtc gttcgtgttg cagaaggaat
     6361 ttggctttag tccaatgcaa tttagcctgg tgtttggcct taacggcacc ggacttatca
     6421 ttgcttcatg gatcttctcg cgcctggcgc gacggattaa cgcgatgaca ttgttgcgag
     6481 gtggcctgat agcggcaatt ttgtgtgcat tgctcacggt cttatgcgca tggatacaat
     6541 tgcccattcc ggcactggtg gcattatttt tcaccatcgc attttgtagc ggcatcggca
     6601 ctgttggcgg ggcagaggct atgagtgcag tagggacgca ggaatctgga acggcgtctg
     6661 cgttgatggg gatgagcatg tttgtcttcg gcggtatagc cgcgccattg tcgggaattg
     6721 gcggagaaac actgttaaaa atgagtctgg caattacggt gtgttatacg ctggcattgc
     6781 tggttgctct caccagaatc gacaaccaaa agtaatgaac acctcatcct gaggggggcc
     6841 tgtcaggatg aggtgcaaaa cactaacggt ccttacgcca cgcatccgcc gtcaatgcct
     6901 cgccaaaatg accggcgatc agccgtttgg tgagttcatg cagcggcgat gccagcacat
     6961 ctgcggtgct gcctcgctcg acaacctcgc cctgatgcat caccagcacc tggtcgctaa
     7021 tgtgcttcat cattccgata tgctgggtaa cataaatata cgaaatgccc tgtttttcct
     7081 gtaactccag catcagatta atcaactgcg aacgcatcga catatccagt gaggcgaggg
     7141 cttcatcggc aataatgact tttgggcgca atatcagcgc gcgcgccaga cccagacgct
     7201 gtttttgccc gggtgccaac atatgcggat agtaactgac gtgatccggt agcagcccaa
     7261 ccatacgcat cgtttcaata atctgtttgc gacgctgttc cggttccagg tcggtgttca
     7321 ggcgcagtgg aaaatccaga atttgcgaga tacgttgacg gggattcaac gaggtggaag
     7381 gatcctgaaa aatcatgcga atacgctgac tgcggaagga ataatcgcca aaatgcagcg
     7441 gatgatcgtc aatcaataac tcgccgctgg taggttctat cattcccgcc agcatttttg
     7501 ccagcgtgga tttacccgaa ccattctcgc cgataatcgc cagtgtctgg ccttcacgta
     7561 gcgtaaagct caagggtttt accgcttcta cggtctgacg acgaaaccag ccggtccggt
     7621 agcggaacgt tttacttaaa ttacgcactt caagtagcgt ttcgatcatc tcactctttc
     7681 tccatgttca gtgggaaatg acaggcatag agatgatttt tcgcccccgt caaacgtggc
     7741 gtcacaatgc attctcgttg tgcatacggg caacgtggcc ccagacgaca accaatcggt
     7801 aactgttcca gtagcgggat agcgccgggc agcgtattga ggcgactttt atgcggcatc
     7861 gcgctgccga agtctggtat cgcgcggatc agcgcctggg tataaggatg gtgtggcatc
     7921 gtcaccaact ccttactcgg cgcggtttcc accgtttgac cgcagtaaag cacgttaatt
     7981 ttatccgccc attggctaag catttgtaag tcatggctga taagcaaaat agtggtattg
     8041 ctgttttggt tgagacgcgt caggaggcga aagatttgcg cctgggttgt tggctccatt
     8101 gagtttgtcg gttcgtcagc aatcagcaga cgcggttgat tcgccagtgc aatggctatc
     8161 atcactttct gacattcgcc ttcggtcaac tcatagggaa aactgcgcat cgcatctttg
     8221 tgatctttaa tccccacgcg gtgcagcagt tcaatggcac ggcgtttgcg ccagccaaaa
     8281 cgctgccacc aacggccttt ataggtccag gctgggatgt tttgcatcaa ctggcgaccc
     8341 acacgttctg aagggtcaag acacgactgc ggttcctgga aaatcatcga cacgttgtga
     8401 ccaaccagct tgcgccgttc gcgtgcggag agacgcagca aatcgatatc atcaaaacgc
     8461 atacggtcag cagtaacacg ccagttatct ttattcaccc cacaaatcgc tttcgcaatc
     8521 aaacttttac cggaaccgga ttcgccaaca agaccgcgga tttcaccttc ggttaaggtc
     8581 atacttacgc ggtcgacggc tttaacccac tcatcaccgg ttttaaattc aatggtcagg
     8641 ttacgaatat cgagtaatgg cattattcca cccccgcaat aattgcacga cggacaccgt
     8701 cgccaaggag gttaaccaac agcacgctaa tcataatcgc cgcaccaggc agcatgacag
     8761 tccacggggc gacataaatc agttccagcg catcaccgag catcgctccc cattcaggcg
     8821 agggcagttg tgcgccgaga tcgagaaagc ccagcgcggc gatatcgaga attgccatcg
     8881 acagtgcgcg ggtgatctcg gtgaccagcc cggcggtgat gttgggcatc acagcaaacc
     8941 agagaatatt cagcgttgat gcgccatcca gacgggcggc gataacgtac tctttttcca
     9001 gttcgtcatg caccatgctg taaatcgaac gtaccatacg tggcagcagc gccagccaga
     9061 cagcaaacat ggcgtgagac aaactcggtc cggcaaacgc tacaacgata attgccagca
     9121 gcagcgaagg gatcgccagt aaggtatcca gaatatggtt aagcaccgct gagcgtaggc
     9181 cgtgggtcgc cccggcgaag gtgcccagta ccaggccaca gatcgtcgcg gcaagcgtca
     9241 ccacaaatgc gccacctacg gttggcgcag ctccgctcag taaacggctt aaaacatcgc
     9301 gtccgaggtc gtcagtcccc aggaagaaag aaacttcgcc atagcgcgac catgacggcg
     9361 gcaataattg ataaccgaga aattgctggt cgatgccgta aggcgcaaac cagccgccaa
     9421 aaatacacag tacagccagc cccgcgcagc cgtacaggcc gaccatcgca gaggcgtcac
     9481 tataaaattt gcgccaggcg gtacgcagcg tgcccggcgg gcgcttttcg ctgtatacgc
     9541 tatcgtaagg cataccattc cttatgtttc agagggttag ccatggcacc caaaatatca
     9601 gaaatcacgt taacaataat gaccagtgag ccacacacca tcactccggc ggaaatggct
     9661 gcgtaatcct gctggcggat ggggttaatt aaccagcgcc ctaaacccgg ccagctaaaa
     9721 accatttcgg tgatcatcgc cagcgtcagc atggtggaaa actgtaagcc caggcgagga
     9781 ataaccggag gtaacgcgtt atgcagaacg tggcgacgca aaatagtaaa gcgcgacaaa
     9841 ccgcgggtcg ccgctgcttt aacatagttc tggtcataca cttcgatagt gctgatacgc
     9901 atcagtcgga tcacttcagt tgttggcgca accgacaggg tgatcacggg caataccata
     9961 tggcggattg cgctaatgat catttcatcc cgccacggtg agtccgaaag ccaggcatca
    10021 atcaacgcaa aaccggtaat cggtttcact tcgtagagca gatcgaaacg ccctgaaacg
    10081 ggcaaccaac ctagcgtgag tgaacaaaac agcgtcaaca gaagcgccag ccagaacacc
    10141 gggattgaaa agcccagtaa ggcgatggcg ttgatcaagt tatcctgcca cttatggcgc
    10201 gtaatcccgg caatcatccc cacggggatg ccaacaatca gcgcaaaacc gaaggcgagg
    10261 atgcacagtt ccatcgttgc cgggaagacc tcttttaact gttcggcaat cggttgaccg
    10321 ttaatgctgg aaacaccaaa atcccagtgg atcaggccat taaaccagaa cacccaggca
    10381 ttccacagtg acgcgccttg taacggcgca tgtggggtga aatagctcaa gctgaagcca
    10441 acgaaggtca gcaggaacaa ggtcacaatc aataacaaaa tacggcgtaa ggtgaagata
    10501 atcatggctt tttcacctca tcctgtttct cgcgatacac cccagcaaag gaggcgttac
    10561 caaacgggct aagtaccaga cctttgatat cgtaccgata ggcctgcaaa cgcaatgacg
    10621 acgccagcgg cagaataggt aattcctgcg ccagaatact ttgcgcttcg tcataggctt
    10681 caatacgcgc cgccagctgc tgcgaggaga gcgccttacg caacacgctg tcgaatttcg
    10741 gatcgcacca gtgggcgagg ttggtctgcg aatgaattgc cgcgcagctc agtaacggac
    10801 ggaagaaact gtccgggtcg ttactgtccg tcgcccaacc ggataacgtc agatcatggc
    10861 tcatatccat caaccgcgcc tcctgaaaac gaccttctac cggcacaatc accactttta
    10921 cgccaacctg tgccatatcc gcctgaatca gttcggcggt tttcagtgga ctggggttcc
    10981 acgcctgcga acgtgtgggc acccacagtt tcagcgttaa gttttccagc cccaacgcct
    11041 tcaactgttc gcgcgatttt gccggattgt attcagtaat tttagcctcg ttgtcatagg
    11101 cccacgaggc gcgcggtaaa atagaggccg ctgtttcagc cgtaccataa tagatggatt
    11161 gcatcaggcg ctggttatta attgccagtg ccagcgcatg gcggacagcg ggattattta
    11221 gcggcggttt ggcggtgttg aatgccagat aggcgacgtt catcccagga cgcagcgtta
    11281 aacgcaagcg tgggtcgtca cgcaaaatgg atagctgact ggcagcaggc caggccagaa
    11341 cgtcgcactc cccggtcagg agtttcgaca atcgtcctgt accgccagag cctaaatcca
    11401 ccaccacttg tggcatcaac ggcttaccac gccagaaatc atcatgacgt tgtaagcgaa
    11461 tatattgccc ggcgcggtat tccgacaact gatacggacc ggtgccgacc ggttgacggt
    11521 cgagttgctc ctggcgatct tctttctcta acttccctgc atattctgcc gacatgaccg
    11581 aagcataatg ggtcgcgagg tgccacaaaa aagaagcatc cggctgagcc agacggaact
    11641 caacggtatg attatccagt ttgcggacgc ttttcacgtt atcggcaaat tgcaggctgt
    11701 cgaagtaggg gaagttgctg ccattaacgt tatgccacgg gttgttgcgg tcaaaaattc
    11761 gctggaaggt aaaaaccaca tcgtcggcat tcattttacg agtgggagta aaccagtcgg
    11821 ttttttgaaa cggaacatcg cgacgcaggt ggaagcgata ggttgcgccg ttgtcgagta
    11881 cttcccagct ttcggcaagt tccggcatca gacgataggt atagggatcg acatccagca
    11941 gtcgatcata aaactgggcg gcaagggtat cgacaattaa cccactgctc gctttggatg
    12001 ggttaaaggt gttgacttgc ccgctgacgc aatagacaaa accgctgtcg cggatatcag
    12061 catgcggggg ggattcaggc gcggcgattg cctgaccact cacaagtcca gcaatcacca
    12121 aaagagacga taatacctgg cgcataatat taagggattt tatgtaaaga ggctatctta
    12181 ctaatattta atgacatttg ccattaccgt ttgtgttcag aggtaataat gagggccgtg
    12241 tggttggtca ttgaacaggt ggcgtcgcca tcagttcaac attcgtaccc gtgatttctt
    12301 tataactata actcacagaa caacttagcg aggagcagag cggtacgatc gtgagaaacc
    12361 cattaaagca cgctgcagtg cgtgtcattt ttagccggat gcggcgcgaa cgctttatcc
    12421 gggcaacgat actgaccgat agtctgcaat taaataatta ctcattaccc cattgattca
    12481 aaaactctgc gatctcatca atgcgtacgg gattaatccc cgcttcagca gccatttcat
    12541 gttgggcttc ttcactgatc tcttcattgt tcatcaaacg ggtgagcagt aactggaagt
    12601 agtgggccag cggtgtgcgt tcagcgtctg cgacaggctc tgcgcattcg gtcgcgtact
    12661 catcggcaat atcataatat tttagcggga tatcgtggtt cattatttgc ccctggggtt
    12721 aatgcgctaa gcataagggc ggatgatagc actcgtgatc cgggtcatac atatttttat
    12781 agttaatggc gcggtgcatt accctcaggc aggataatgc gccgcgcatt cgactattac
    12841 gtttcgctca ccggcgttct gtcgccatca tacaatggca ctttgcgata gcggcggatc
    12901 aaataccaca aatatgcgcc gcctaacgaa gcccatacca tcccggttgc gcaattcgat
    12961 gcggcaatga aaattttgca ggatgcgctg agagattaat acgcagaagc agggttaacg
    13021 caataatgcc tgatgcgcag cgcttatcag gcctgcaaac gacgtattga ttttgtaggc
    13081 ctgataaggc gttcacggca cacccggcaa gttgcattgt cgaaatgcta aagctggtgc
    13141 tttttcagca acgcgcggaa ctgatgatag gttaacccca gcaattcagc ggcgcgcttc
    13201 tggttatact tcccttgttc caagctgacc tgaagcagtt ctttttcctg ctgcatctga
    13261 aactcgcgta aatccagcgg cagcgtcgga agcgaggtgt tttctgaaac ggcgatggct
    13321 tcttcagatg gacggcgttt aaagggatca ataatgatgt catcaagcgg ataatcgctg
    13381 gtgccgtggc gatacactga acgttccacc acgtttttca attcacgtat atttcccggc
    13441 cagcgataat tcagcaatgt ttctctggcg tgctcggtaa aacccgggaa cagaggcagc
    13501 ttgatttccc gacacatctg gatggcaaag tgttctgcca tcaacataat gtcgctttca
    13561 cgttcgcgta gtggcggcag ttgcacaaca tcgaacgcta gtcggtcgag caggtcagcg
    13621 cgaaaagtcc cttcattgac cattgccggg agatcggcat tcgtcgcgca taccaaccgc
    13681 acattcacct gcaatggctg gctgccgccg acgcgctcca gttcaccgta ctcaatcaca
    13741 cgcaataatt tttcctgcac catcatcggt gccgtagcga gttcatcaag aaatagcgta
    13801 ccgccgtcgg cacgttcaaa tctccctgga tgacgttttt gcgcaccggt aaacgccccc
    13861 gcttcgtgac caaacagttc ggaatccagc aaattttcat ttaacgccgc gcagttaagg
    13921 gaaataaacg gcccttgcca acgggaggag agataatgca gacggctggc aatcagctct
    13981 ttaccggtgc cgcgctcgcc gatgatgagc accggtttgt ccagcggtgc gagatgcgaa
    14041 acctgttcca gcacttcgag aaagctgttc gcctcaccga gtaaattatc tttgtattct
    14101 gccatgatga aattcgccac ttgttagtgt aattcgctaa ttcatcctgg catgttgctg
    14161 ttgattcttc aatcagatct ttataaatca aaaagataaa aaattggcac gcaaattgta
    14221 ttaacagttc agcaggacaa tcctgaacgc agaaatcaag aggacaacat tatgggtatt
    14281 ttttctcgct ttgccgacat cgtgaatgcc aacatcaacg ctctgttaga gaaagcggaa
    14341 gatccacaga aactggtgcg tctgatgatc caggagatgg aagatacatt ggttgaagta
    14401 cgttctactt cggcgcgtgc gctggcagaa aagaaacagc tgactcgccg tattgaacaa
    14461 gcgtcggctc gtgaaattga atggcaggaa aaagcggaac tggcactgct gaaagagaga
    14521 gaggatctgg cgcgtgcggc gttaattgaa aaacagaaac tgaccgatct gattaagagc
    14581 ctggaacatg aagtgacgct ggtggacgat acgctagtgc gtatgaagaa agagatcggc
    14641 gagctggaaa acaaattgag cgaaacacgc gctcgccagc aggcattgat gttacgccat
    14701 caagcggcaa actcgtcgcg cgatgtgcgt cgtcagctgg acagtggcaa actggatgaa
    14761 gcaatggctc gtttcgaatc tttcgaacgc cgtatcgacc agatggaagc ggaagcagaa
    14821 agccacagct tcggtaaaca aaaatcgctg gacgatcagt ttgccgaact gaaagccgat
    14881 gatgcaatca gcgagcaact ggcacaatta aaagccaaaa tgaagcaaga caatcaataa
    14941 taacatccag gcggcgtccg aacgcgccgc cgctcatcgt ctaaggagta cttatgagcg
    15001 cgctatttct ggctattccg ttaaccattt ttgtgctgtt tgttttaccg atctggttat
    15061 ggctgcatta cagcaatcgt tccggtcgca gtgaattgtc acaaagtgag cagcagcgat
    15121 tagcgcaact ggctgatgaa gcaaaacgga tgcgcgagcg tattcaggcg ctggaatcca
    15181 ttcttgatgc agaacatccg aactggaggg atcgctaatg gcgggcatta atctcaataa
    15241 aaaattatgg cgtattccgc agcagggcat ggtccgtggc gtctgcgccg ggattgccaa
    15301 ctattttgat gtaccagtaa aactggtgcg tatcctggtg gtgctgtcga ttttcttcgg
    15361 tctggcgctg tttaccctgg ttgcttacat cattttgtca tttgcgcttg atccaatgcc
    15421 ggacaacatg gcctttggtg agcagctacc ttccagcagc gaattgctgg atgaagtcga
    15481 ccgtgaactg gcggcaagtg aaacgcgttt acgcgagatg gaacgttatg tcacttccga
    15541 cactttcacg ctgcgtagcc gtttccgtca actgtgagga aagttatgaa tactcgctgg
    15601 caacaggccg ggcaaaaggt gaagcctggg ttcaaattag caggcaagct ggtacttctt
    15661 accgcactgc gctatggccc ggcgggtgtg gcgggctggg cgataaaatc agttgctcgc
    15721 cgaccgctga aaatgttgct ggctgtggcg ctggaaccgc tgttaagtcg ggctgccaat
    15781 aaactggcgc agcgttataa aaggtgaggg gagtttcgca aaaatggtta aatctcaggc
    15841 gtataatgga tagcaatttt catccataga aggacgctta catgtttaaa aaaggcttac
    15901 ttgctctgac actggtgttt tcactgcccg ttttcgccgc tgaacactgg atcgatgttc
    15961 gtgttccaga gcagtatcag caagagcacg ttcagggggc catcaatatt cccctgaagg
    16021 aagtgaaaga gcgcattgct accgccgttc cggataaaaa cgacaccgtg aaagtgtatt
    16081 gcaatgccgg acgccagtca gggcaggcaa aagagatcct tagcgagatg ggatataccc
    16141 acgttgagaa cgccggtggc ctgaaagaca tcgcaatgcc gaaggtcaaa ggttaaaaga
    16201 attcaccatg agcggcgctt atgcgccgtt tttttctgtc acaccttatt tacatcccca
    16261 tagattattt gcgtcagctc acaaatacgc tttttcccag gtaaaaaaag atttcctgcg
    16321 tgactaaacc ccttgagctc aattgacagt ttattttatg cggagtagtc tctcgcttca
    16381 tgggaccgct accacggaaa ggcaacatgc aacagaaaat tacggattac ctggacgaaa
    16441 tctacggtgg aacatttacc gcgactcatt tacagaaact tgtaacgcgt cttgagagtg
    16501 cgaaacgatt aattacacag cgacgtaaaa aacactggga tgaaagtgat gtcgtgttaa
    16561 ttacctatgc cgatcaattt cacagtaatg atttaaagcc actacccaca tttaatcagt
    16621 tttaccctca atggctgcaa agaatttttt cacatgttca tttattacct ttttatccat
    16681 ggtcatctga tgatggattt tcggtaattg attatcatca ggtcgccagt gaagcggggg
    16741 agtggcagga tattcagcaa ctcggtgaat gcagccattt aatgtttgat tttgtctgca
    16801 accatatgtc ggcaaaaagt gaatggttta aaaactattt acaacagcag ccaggttttg
    16861 aagatttttt tattaccgtt gatccgaaaa ccgatctcag cgccgtcact cgcccgcgtg
    16921 cgttaccgtt attaacgcca ttccagatgc gcgatcattc aacgcgtcat ttatggacca
    16981 cctttagcga cgatcaaatt gacctcaatt accgtagccc tgaagtgttg ctggcgatgg
    17041 tggatgtatt gctgtgctac ctggaaaagg gcgcagagta tgtccgtctg gatgccgttg
    17101 gctttatgtg gaaagagccg ggaacaaact gcatccatct ggaaaaaaca catctgatta
    17161 tcaaactgtt acgttcgatt attgataacg tcgcgccagg cacagtgatc attaccgaga
    17221 ccaatgttcc gcataaagac aacattgctt actttggcga aggcgatgac gaagcgcata
    17281 tggtgtacca gttctcgctg ccgccgctgg tgctgcatgc ggtgcaaaaa cagaacgttg
    17341 aggcactttg tgcgtgggcg caaaacctga cactaccttc cagcaacacc acctggttta
    17401 acttccttgc ctctcacgat ggcatcgggc ttaacccact gcgggggttg ttacccgaaa
    17461 acgaaatatt agcgctggtc gaggtgttac agcaggaagg ggcattagta aactggaaaa
    17521 ataatcccga cggtacgcgt agcccctatg aaatgaatgt cacctatatg gatgcgttga
    17581 gccgccgtga gtgtagcgac gaagaacggt gtgccaggtt tattctcgcc catgcgattt
    17641 tgttaagttt ccccggtgtg ccagcgatat atattcaaag cattctgggg tcgcgtaatg
    17701 attacgccgg tgtcgaaaaa cttggatata atcgcgcgat taaccgtaaa aaatactcta
    17761 gtaaagagat aacgacagaa ctggacaata aagcgacatt aaggtattcg gtatattata
    17821 agttgtcgca tttaattacc cttcgtcgca gtcataaaga gtttcatccg gataataatt
    17881 tcaccatcga tgcaattaat tcatttgtaa tgcgaattca aagaagcaat gcggatggcg
    17941 attgcctgac gggattgttt aatgtcagcg aaaatattca gcatataaat attactgacc
    18001 tgcacggtcg ggatctgatt agtgaagttg atatattggg taatgaaata acactgcatc
    18061 cctggcaggt tatgtggatt aaataaaaag gaacatttca tgattaaatc aaaaatcgtg
    18121 ctgttatcag cactggtttc ctgcgccctg atttcaggct gtaaagaaga aaataaaaat
    18181 aatgtatcca tcgaatttat gcattcttcg gtggagcagg agcgccaggc cgtcatcagc
    18241 aaattgattg cccgttttga aaaagaaaac ccaggtatca ccgtaaaaca agtgcccgtg
    18301 gaagaagacg cctataacac caaagtcatt accctttcac gtagcggttc gctgccggaa
    18361 gtgatcgaaa ccagccatga ctacgccaaa gtgatggaca aagagcagct tatcgatcgt
    18421 aaagcggttg ccacggtcat cagcaacgtc ggtgaaggcg cgttctacga tggcgtactg
    18481 cggattgtgc gcaccgaaga tggcagcgcc tggaccggtg ttcctgtcag cgcatggatt
    18541 ggcgggatct ggtatcgcaa agatgtgctg gcaaaagctg ggctggagga gccaaaaaac
    18601 tggcagcaat tgctggacgt tgcacagaaa ctgaatgacc cggcgaataa aaaatacggt
    18661 attgcgctgc ctacagcaga aagcgtgttg acggaacagt ctttctccca gtttgcgtta
    18721 tccaatcagg ctaacgtctt taacgccgaa ggcaaaatca cccttgatac accagagatg
    18781 atgcaggcgc tgacctatta ccgcaacctt gctgccaaca ccatgccagg ttctaacgac
    18841 atcatggaag tgaaagacgc ttttatgaac ggcaccgcgc cgatggcgat ttactccacc
    18901 tatatccttc cggctgtgat taaagaaggc gatccgaaaa acgttggttt cgtggtgcca
    18961 accgagaaaa attctgcggt ctacggcatg ttgacctcgc tgaccattac cgccgggcaa
    19021 aagactgaag agaccgaagc ggctgaaaaa tttgtcacct ttatggagca ggcggacaac
    19081 attgccgact gggtgatgat gtcaccaggc gcggcgctgc cggtgaacaa agcggttgtc
    19141 actaccgcca cctggaaaga caacgacgtt attaaggcgc tgggagaatt gccgaatcag
    19201 ctaatcagcg aattgccaaa tattcaggtc tttggcgcag taggggataa aaactttacc
    19261 cgcatgggtg atgtgacggg gtctggtgtg gtgagttcaa tggtgcataa cgtcaccgtg
    19321 ggtaaagccg acctccctgg cacgctgcaa gcgagccaga aaaagctgga tgagttggtc
    19381 gaacagcgct aaacccagga caggaatccg caatgaacag gcttttttca ggtcgttccg
    19441 atatgccctt tgcgctgttg cttctcgcgc ccagcttatt actgctgggc ggtctggtgg
    19501 cgtggccgat ggtgtcgaat atcgaaatca gttttttacg tctgccgctt aatcccaaca
    19561 tcgagtcaac gtttgttggg gtgagtaact atgtgcgcat tctctccgat cctggtttct
    19621 ggcactcgct gtggatgacg gtctggtata ccgcgcttgt ggtggcgggc agcaccgtgc
    19681 tcgggctggc cgtggcgatg tttttcaacc gcgagttccg cctgcgcaaa accgcgcgtt
    19741 cgctggtgat cctttcctac gtaacgccgt ctatttcgct ggtgttcgcc tggaaataca
    19801 tgttcaacaa cggctacggc attgtgaact acctcggcgt cgatcttctg catctctatg
    19861 agcaggcgcc gctgtggttc gataacccgg gcagtagttt tgtgctggtg gtgctgttcg
    19921 ccatctggcg ctacttcccg tatgccttta tctcgtttct ggcgattttg cagaccatcg
    19981 acaaatcgct gtacgaagcg gcagagatgg atggcgcgaa tgcctggcaa cggtttcgca
    20041 tcgtcacgct gcccgcgatt atgccggttc tggcgacggt ggtgaccctg cgcactatct
    20101 ggatgttcta catgttcgcg gatgtttatc tgctgacgac caaggtcgat attctcggtg
    20161 tctatctcta caaaaccgcc tttgccttta atgacttagg gaaagcggcg gcgatctcgg
    20221 tggtgctttt catcattatt ttcgctgtca ttctgctgac caggaaacgg gtgaacctca
    20281 atggcaacaa ataaacgcac actcagtcga atcggttttt actgcgggct ggcgctgttt
    20341 ctcatcatca cgctgtttcc attttttgtg atgctgatga cctcgttcaa gagcgcaaaa
    20401 gaggcgatct cgctgcatcc tacgttgctg ccacagcagt ggacgctgga gcattacgtc
    20461 gacattttta atccggtgat tttcccgttt gtcgactact tccgtaacag catggtggtg
    20521 tcggtggttt catccgtggt ggcggtattt ctcggcattc ttggggctta cgcgctttcc
    20581 cgcctgcgct ttaaagggcg aatgaccatc aacgccagct tttacacggt gtacatgttc
    20641 tccggcattt tgctggtggt gccgctgttc aaaatcatca ccgcacttgg catttacgac
    20701 accgagatgg cgctgattat caccatggtg acgcaaactc tgcctaccgc cgtgtttatg
    20761 ctgaaaagct acttcgacac catccccgat gaaatcgaag aagcggcaat gatggatggc
    20821 ctcaatcgtc tgcaaattat cttccgtatc accgtgcccc tggcaatgtc cgggctgata
    20881 tccgtattcg tctactgctt tatggtggcg tggaacgact atctgtttgc gtcgattttc
    20941 ctttccagcg ccagcaattt caccttacct gtgggcctga acgcgctgtt cagtacgcct
    21001 gattacatct ggggacggat gatggcggcc tcgctggtga ccgcactacc ggtagtgatt
    21061 atgtatgcgc tttcggaacg ttttattaag agtggtttga ccgctggtgg cgtgaagggc
    21121 taaagcgacc aatttcttta caaggagttt tacatgaaaa agttagtagc cacagcaccg
    21181 cgtgtagctg cgctggttga gtatgaagag cgggcgattt tagccaatga agtgaagatc
    21241 cgcgtgcgtt tcggcgctcc gaagcacggg acggaagtgg tcgatttccg cgctgccagt
    21301 ccgtttatcg atgaagactt taacggtgaa tggcagatgt tcagcccgcg tccggcaaat
    21361 gcgccgcgcg gcattgagtt tggcaagttc cagcttggca acatggtggt tggcgacatt
    21421 atcgagtgcg gcagcgacgt taacgactac gcggtgggcg acagcgtatg cggctacggc
    21481 ccgctctccg agacggtcat cattaacgca gtgaataact acaagctgcg caaaatgccg
    21541 caaggcagct cctggaaaaa cgccgtctgt tacgacccgg cgcagtttgc catgagcggc
    21601 gtgcgcgatg ccaacgtgcg cgtgggcgat tttgtggtgg tggtagggct tggcgcgatt
    21661 ggtcaaattg ccattcaact ggcaaaacgc gctggcgcgt cggtggtgat tggtgtcgat
    21721 cctatcgctc atcgctgtga tattgcccgt cgccacggtg cggatttctg tcttaacccc
    21781 attggcgctg acgtaggtaa agagatcaaa acgctgaccg gcaagcaggg tgccgatgtg
    21841 attatcgaaa ccagcggcta cgccgacgcg ctgcagtcgg cgctccgcgg tctggcctac
    21901 ggcggcacca tctcctatgt cgcgtttgcc aaaccatttg ctgaaggttt taacctcgga
    21961 cgcgaagcgc atttcaataa cgccaaaatt gttttctccc gcgcgtgcag cgaaccgaac
    22021 ccggattatc cgcgctggag ccgtaagcgt attgaagaaa cctgctggga actgctgatg
    22081 aacggttatc tcaattgcga agatttaatc gacccggtag tgacctttgc caacagcccg
    22141 gaaagctaca tgcagtatgt cgatcagcat ccggaacaga gcatcaaaat gggcgttacg
    22201 ttttaattca aaggaatgaa aaaatgaaaa taggcacaca gaatcaggca ttctttccgg
    22261 gaaatattct ggagaaattt cgttacatca aagagatggg cttcgatggt tttgagattg
    22321 acggcaaact gctggtcaac aacatcgaag aagtcaaagc ggcgatcaaa gaaaccggtt
    22381 taccggtgac cactgcctgc ggcggctatg acggctggat tggtgatttt atcgaagagc
    22441 gtcgtcttaa tggcttaaag cagatcgaac gcattctcga agcgctggca gaagtgggcg
    22501 gtaaaggtat cgtcgttcct gctgcatggg gcatgtttac cttccgttta ccgccgatga
    22561 cctcgccgcg cagcctggac ggcgaccgca aaatggtgag tgattccctg cgcgtactgg
    22621 aacaggtcgc cgcgcgtacc ggaaccgtgg tgtatctcga accgttaaac cgttatcagg
    22681 atcatatgat caatactctc gccgatgccc gccgttacat cgtcgaaaac gatcttaaac
    22741 atgtgcagat tatcggcgat ttctatcaca tgaacatcga agaagataac ctggcgcagg
    22801 cgctgcatga ccaccgcgac ttgctcggtc atgtgcatat tgccgataac catcgttacc
    22861 agccgggcag cggcaccctc gatttccacg cgctgtttga acagctgcgc gccgataact
    22921 atcagggtta tgtggtgtat gaagggcgta tccgggcaga agctcctgcc caggcgtacc
    22981 gtgattcgtt ggcctggttg cgcacctgct aagaggtctt tgtgaaaagt gcaatgacaa
    23041 gctctccgct gcgtgtcgcg ataatcggcg caggccaggt ggcggataag gttcatgctt
    23101 cgtactactg cacccgcaac gatctggaac tggtggccgt ctgtgacagc cgcctttccc
    23161 aggcgcaggc gctggcagaa aaatacggta atgcatcctt gtgggacgat ccgcaggcca
    23221 tgctgctggc ggtgaaacct gatgtggtta gcgtctgctc accgaaccgt tttcattacg
    23281 aacataccct gatggcgctt gaagcgggct gccatgtgat gtgcgaaaaa ccgcccgcca
    23341 tgacggcaga acaggcgcgg gaaatgtgcg ataccgcgcg caaacagggc aaggtgctgg
    23401 cctacgactt tcaccatcgt tttgcgttcg atacgcaaca gctgcgtgaa caggtgacca
    23461 acggcgtttt gggagagatt tacgttacca ccgcccgcgc cctgcgtcgc agcggcgttc
    23521 ccggctgggg tgtctttacc aataaagaac tgcagggtgg tggcccgctg atcgacatcg
    23581 gcattcatat gctggatgct gcgatgtatg tgctgggctt tccggcggtg aaaagcgtga
    23641 atgcgcatag ctttcaaaag atcggcacgc aaaagagctg tggtcaattt ggcgagtggg
    23701 acccggcaac ttacagcgtt gaagattcgc tgtttggcac cattgaattt cataacggcg
    23761 gcattctgtg gctggaaacg tcatttgcac tcaacatccg cgaacagtcg attatgaatg
    23821 tcagcttttg tggtgataaa gccggtgcga cgctgtttcc ggcacatatc tacaccgata
    23881 acaacggtga attaatgacg ctgatgcaac gggaaatggc agacgacaac cgccatttgc
    23941 gcagcatgga agcctttatc aatcacgtac agggcaagcc cgtgatgata gccgacgccg
    24001 agcaggggta catcatccag caactggtgg cggcgttgta tcaatccgcg gaaacaggga
    24061 cgcgtgtgga attatgacca ggccagtaac gttatcagaa ccccatttca gccagcatac
    24121 cctgaacaag tatgcttcgc tgatggcgca ggggaacggc tatcttgggc ttcgtgccag
    24181 ccatgaagaa gattacacgc gccagacccg tgggatgtat ctggcggggc tgtatcatcg
    24241 agcgggtaaa ggagaaatca acgaactggt gaacctgcct gatgtcgtgg ggatggagat
    24301 tgccataaat ggtgagattt tctcgttatc ccgcgaagca tggcagcgtg agcttaactt
    24361 tgccagtggc gaattacgtc gcaacgttgt ctggcgtacc agcaacggca ctggttacac
    24421 catcgccagc cgtcgctttg tttcggcaga ccaactgccg ctcattgcgc tggaaatcac
    24481 tattactcca ctggacgccg acgcgtcagt gctgatttca acaggcattg acgccacgca
    24541 aaccaaccac ggtcgccagc atattgacga aatccaggtg cgggtgtttg gtcagcatct
    24601 gatgcagggg atctacacca cccaggatgg gcgtagtgat gtggccatca gctgttgctg
    24661 taaggtgagc ggtgatgtgc agcaatgcta taccgccaaa gagcgccgtt tattgcaaca
    24721 tacctgcgcg cagcttcacg caggtgagac gctgacgctg caaaaactgg tgtggatcga
    24781 ctggcgggac gacagacaag ccgctttaga cgaatggggc agtgcgtcgc ttcgtcagct
    24841 tgaaatgtgc gcgcagcaga gttacgacca acttcttgca gcatcaacag aaaactggcg
    24901 tcaatggtgg cagaaacgtc gtatcacggt aaacggccgc gatgcgcacg atcagcaagc
    24961 attagattat gcgctttatc acctgcgcat catgacgcct gcccacgatg agcgcagcag
    25021 cattgcggcg aaaggtttaa ccggcgaagg ctacaaaggc cacgttttct gggatacaga
    25081 agtatttttg ctaccgttcc atctgtttag cgatccgacg gttgcccgaa gtttactgcg
    25141 ttatcgctgg cacaacttgc ctggcgcgca ggagaaagcg cgacgcaacg gctggcaggg
    25201 cgctctgttt ccgtgggaaa gcgcgcgtag cggcgaagaa gagacgccgg aatttgccgc
    25261 cattaacatt cgtaccgggc tgcggcaaaa agtggcctct gcgcaggcgg aacaccatct
    25321 ggtcgccgat atcgcctggg cggtgattca atactggcgg accacggggg atgaaagttt
    25381 cattgctcat gaaggcatgg cgctacttct ggagacggca aagttctgga ttagccgcgc
    25441 ggtgagggtt aacgagcgtc tggaaattca tgatgttatt gggccagacg aatataccga
    25501 acatgtcaat aataacgcct tcaccagcta tatggcgtat tacaacgtcc agcaggcgct
    25561 gaatatcgct cgtcagtttg gctgtagcga cgacgcgttt attcatcgtg cagaaatgta
    25621 cctcaaagaa ctgcggctgc cagaaattca gcccgacggc gttttgccgc aggatgattc
    25681 gtttatggct aagccggtga ttaatctggc gaaatacaaa gcggcggcgg ggaagcaaac
    25741 cattctgctg gattattcac gcgcagaagt gaacgagatg cagatcctca aacaagctga
    25801 tgtggtgatg ctcaattaca tgctgccgga gcagttctca gcggcatcgt gtcttgccaa
    25861 tctgcaattt tatgaaccgc gcactattca cgactcgtca ttaagtaaag caatccacgg
    25921 cattgttgcc gcacgctgtg gcctgctgac ccaaagttat cagttctggc gcgaggggac
    25981 tgaaatcgat cttggtgctg atccgcatag ctgtgatgac ggtatccacg ctgccgcaac
    26041 tggcgctatc tggctggggg cgattcaggg ttttgccggg gtgagcgtgc gtgacggtga
    26101 attacatctc aatccggcgt tacctgagca gtggcaacag ttgtctttcc ctctgttctg
    26161 gcagggctgc gaattacagg tcactctcga cgcgcagcgt attgcgattc gaacttctgc
    26221 gcctgtttca ctgcgtttga acggtcagct tatttacgtc gctgaagaat ctgttttctg
    26281 tttgggggat tttattttgc ccttcaatgg gaccgctacc acgcatcagg agggtgaatg
    26341 aaactgcaag gggtaatttt cgatctggat ggagtaatca ccgataccgc gcatctgcat
    26401 ttccaggcgt ggcagcagat tgccgctgaa attggcatca gcattgatgc gcagtttaac
    26461 gaattcctaa aagggatcag ccgcgatgag tccctgcggc gaattctgca gcacgggggt
    26521 aaagagggcg attttaaccc acaggaacgg gcgcaactgg cgtatcgcaa aaatctgctt
    26581 tacgtccact cactgcgcga actgacggtt aatgctgtgc tgcctggcat tcacaatttg
    26641 ctggtagatc tccgggcaca gcagattcca gttgggctgg cttctgtctc cctgaatgcg
    26701 ccgacgattt tagcagcgct ggagctgcgc gagtttttca ccttctgcgc ggacgcttcc
    26761 caactcaaaa attcgaaacc ggacccggaa atctttctcg ccgcctgtgc agggctgggc
    26821 gtgccgccac aggcatgtat cggcattgaa gatgcgcagg cgggcattga cgccattaac
    26881 gccagcggta tgcgctcggt ggggatcggc gcgggcttaa ccggggcgca attattgttg
    26941 ccctcaacgg actccctgac ctggccgcgg ttatcggcct tctggcaaaa cgtatagcaa
    27001 aggaatcaac atggcacagc tttcgttaca acatattcaa aagatctacg ataaccaggt
    27061 gcatgtggtg aaggacttca gcctggagat tgccgataaa gagttcatcg tgtttgtcgg
    27121 tccgtcgggc tgcggtaaat cgaccaccct gcgtatgatt gccgggcttg aggagatcag
    27181 cggcggtgag ctgttgatcg acggcaaacg aatgaatgac gttccagcca aagcacgcaa
    27241 tatcgcgatg gtgttccaga actacgcgct gtatccgcat atgacggtct acgacaacat
    27301 ggcgtttggc ctgaagatac aaaaaatcgc caaagagatg attgatgagc gggttaactg
    27361 ggcggcgcaa atcctcggcc tgcgtgagta cctgaaacgc aagccgggcg cgctttccgg
    27421 cgggcaacgt cagcgagtgg cgcttgggcg ggcgattgtg cgcgaagcgg gcgtgttttt
    27481 aatggatgag ccgctctcta acctggatgc caagctgcgt gtgcaaatgc gcgcagagat
    27541 cagcaagctg catcagaaac tgaacaccac catgatctac gtgacccacg atcagaccga
    27601 agcgatgacc atggcgacgc ggattgtcat tatgaaggat gggattgttc agcaggtcgg
    27661 tgcgccgaaa acggtctata accaaccggc gaatatgttt gttgccggat ttattgggtc
    27721 accagcaatg aattttattc gcggcacgat cgatggcgat aaattcgtta cggaaacact
    27781 taaattaacc attcccgaag agaaattagc ggttctgaaa acgcaggaaa gtttacataa
    27841 acccattgtg atgggaatac gcccggagga tattcatccg gatgcgcaag aagaaaataa
    27901 catttccgcc aaaattagcg tggcggaatt aaccggtgcg gaatttatgc tctacaccac
    27961 ggttgggggg cacgagttag tggtccgtgc tggtgcgtta aatgattatc atgcaggaga
    28021 aaatatcact attcattttg atatgactaa atgtcatttc tttgatgcag aaacggaaat
    28081 agcaatttgc taaatacagg gggaaggcat tctcccagga taatacaagg aacaataatg
    28141 aaaaagttat taccctgtac cgcactggtg atgtgtgcgg gaatggcctg cgcacaggcc
    28201 gaggaaaaga acaactggca ctttaatatc ggcgcgatgt acgaaataga aaacgtcgag
    28261 ggttatgtcg aagatatgga tgggctggca gagccttctg tctattttaa tgccgccaac
    28321 gggccgtgga gaatttctct ggcctattat caggaagggc cggtagatta tagcgcgggt
    28381 aaacgtggaa cgtggtttga tcgcccggag ctggaggtac attatcagtt cctcgaaagc
    28441 gatgatttca gtctcggtct gactggtggt ttccgtaact atggttatca ctacgttgat
    28501 gaaccgggta aagacacggc aaatatgcag cgctggaaaa tcgcgccaga ctgggatgtg
    28561 aaactgactg acgatttacg tttcaacggt tggctgtcga tgtataaatt tgccaacgat
    28621 ctgaatacga ccggttatgc tgatacccgt gtcgaaacgg aaacgggtct gcaatatacc
    28681 ttcaacgaaa cggttgcctt gcgagtgaac tattatctcg agcgtggttt caatatggat
    28741 gatagccgca ataacggtga gttttccacg caagaaattc gcgcctattt gccgctgacg
    28801 ctcggcaacc actcggtgac gccgtatacg cgcattgggc tggatcgctg gagtaactgg
    28861 gactggcagg atgatattga acgtgaaggc catgatttta accgtgtggg tttattttac
    28921 ggttatgatt tccagaatgg tctttccgtt tcgctggaat acgcgtttga gtggcaggat
    28981 cacgacgaag gcgacagcga taaattccat tatgcgggtg ttggggtgaa ttactcgttc
    29041 tgataatgtg ctaaattgcc ggatgcgggg cgagcgccaa atccggcata catttatttc
    29101 aacgacatat gcgtagcaac ggtaatattt tgtggtgacg gttttccggc aattaactgg
    29161 aataataact cgcagctttg ttgacctaac tcctgcgtcg gaacatcgat gccgcctggt
    29221 gcaggcgtta atataaacga cagcgtttca ttactataac ccaccaccgc taactgctgc
    29281 ggaatagcaa tgtttttctc tgccgccgca cgataaatac tcattaattt caggctgtca
    29341 gtagcaaaca ctgcctcagg caacggtgac tggcttaata attcccgtgc tgcttttaat
    29401 gctgtctcat gggtataacc gccgtcaaca atccattcat cacgcactgc aatattatgc
    29461 gtagccagac tttgcttata accatttacc cgatccactg aaacttgtac atcaagcggc
    29521 gcatgcaggc aggctatatt cttatgcccg ctttcaatta acgcattggt cagcgcaata
    29581 ctgtcgccat aattatcggt atcgacggaa taaacatggc tgtattgacc ttcaacttta
    29641 ccgatcacca ccaccgggat ctcgtatttg tcgagttgtg caaagaagga ttcatcggca
    29701 ggcgaactta gcataataat gcctttaatc attttctgct taattttgct ttcgcatttt
    29761 tgcagatctt ctgccggatt gtgcgacgtt tgtaatatca cgtcgaaacc ttcttcttct
    29821 gctttggcgg tgatggcatg caaaacttca gagaaaaatg gattacccgc cgtagttttg
    29881 gtcgaacgag tagaaatcac cataatggca tcaaaacccg aagaggtcag cgcgcgggcc
    29941 agcttgtttg gttgatactg taattcttca atggcccgta acactttttc gcgcgcttcc
    30001 ggggagatat tggtttgctt attcagcacg cgtgatacgg tggattttga tacgcctgca
    30061 accctggcaa tatcataaat agtaggggac ataggtcagg aactccgtcc gaacgtgaat
    30121 gctgcacatc ttatggagtt cgcccgtcga tgacaacacg ggtagttgat aatcaatggc
    30181 ctggccccca cattcatatc cttacgaacg attttttttc tggtcttcag agcagcacag
    30241 gacagcaatg aagcgactta aaaatgaact caatgcgctg gtgaatcggg gtgtcgacag
    30301 acatctgcgc ctcgctgtaa ccggacttag ccgcagcggc aaaacagcgt ttatcaccgc
    30361 gatggttaat cagttgctca atattcatgc cggagcacgt ttgccgcttt taagtgcggt
    30421 gcgtgaagag cgcctgctgg gcgtaaaacg cattccccag cgtgactttg gcattccgcg
    30481 cttcacctat gatgaagggt tggcgcagtt atatggcgat cctcccgcct ggccgacgcc
    30541 tacgcgcggt gtcagtgaaa ttcgcctggc gttacgtttt aaatcgaacg attcgctgct
    30601 gcgccacttt aaggatacct ccacgctgta tctggagatt gtggattacc ccggcgaatg
    30661 gttgctcgac ctgccgatgc tggcgcagga ctatttaagc tggtcgcgcc agatgacggg
    30721 cttactcaat ggtcagcgcg gcgaatggtc ggcgaaatgg cgaatgatgt gcgaagggct
    30781 ggacccgcta gcgcctgccg acgaaaaccg gctggcagac attgccgccg cctggaccga
    30841 gtatctccat cactgcaaac agcaggggtt gcactttatt cagcctgggc gctttgtctt
    30901 gccgggagat atggcaggag cacccgcgct gcaattcttc ccgtggccgg atgtcgatgc
    30961 ctggggcgag tccaaactgg cgcaggccga taagcacact aatgccggaa tgttgcgcga
    31021 gcggtttaat tattactgcg agaaagtggt gaaggggttc tataagaatc attttctgcg
    31081 ctttgaccgc cagattgtgc tggtggattg cctgcaacct ctcaacagtg ggccacaggc
    31141 atttaatgat atgcgtctgg cgctgacgca gctgatgcaa agtttccact acgggcagcg
    31201 taccctgttc cggcgtttgt tttcgccggt tatcgataag ctattgtttg ctgccactaa
    31261 agcggaccat gtgaccatcg atcagcacgc caatatggtt tcattgctgc aacaactgat
    31321 tcaggatgca tggcaaaatg cggcgttcga agggatcagt atggactgcc tggggctggc
    31381 gtctgttcag gcgaccacca gcggaatgat tgatgttaac ggtgagaaaa tcccggcgct
    31441 gcgcggtaat cgactcagcg atggcgcacc gctcactgtt tatcctggcg aagttcccgc
    31501 acgtttgcca ggtcaggcgt tctgggataa gcaaggcttc cagtttgagg cgtttcgtcc
    31561 gcaggtgatg gatgtcgaca aaccgctacc gcatattcgt cttgatgctg cgctggaatt
    31621 tttaatagga gataaattgc gatgaccgaa ccgttaaaac cacgtattga tttcgacggt
    31681 ccactggagg tcgatcagaa tccaaaattc agggcgcagc agacctttga cgaaaatcag
    31741 gcgcaaaatt ttgccccggc cacgctcgac gaagcacagg aagaagaggg gcaagttgaa
    31801 gcggtgatgg acgcagcgtt acgtccgaaa cgcagcctgt ggcgcaaaat ggtgatgggc
    31861 gggctggctc tgtttggcgc aagcgttgtc gggcagggcg tgcagtggac aatgaacgcc
    31921 tggcaaaccc aggactgggt ggcgctgggt ggatgtgccg ctggggcatt gattatcggc
    31981 gctggcgtag gttctgtggt aacagagtgg cggcgcttat ggcgcttgcg acagcgcgcc
    32041 catgaacgcg atgaagcgcg cgatttgttg cacagtcacg gcacgggtaa aggccgtgca
    32101 ttctgcgaaa aactggcgca gcaggcgggc atcgatcagt cacatccggc gctgcagcgc
    32161 tggtatgcct caatccatga aacgcagaac gatcgtgaag tggtcagttt gtatgctcac
    32221 ctggtccagc cagttttaga tgcccaggcg cggcgcgaaa tcagccgctc agcggcggaa
    32281 tcaacgttaa tgattgccgt tagcccactg gcgctggtgg atatggcgtt tatcgcctgg
    32341 cgcaatctgc gtttgattaa tcgcatcgcc acgctgtatg gcattgaact gggatattac
    32401 agccgtttgc gcctgtttaa gctggtattg ctgaatatcg ctttcgccgg agccagcgaa
    32461 ctggtgcgcg aagtggggat ggactggatg tcgcaagatc tcgctgcgcg tttgtctacc
    32521 cgggcagctc aggggattgg tgctggactt ctgacggcac gactcgggat taaagctatg
    32581 gagctttgcc gcccgctgcc gtggattgac gatgacaagc cacgcctcgg ggatttccgt
    32641 cgtcagctta tcggtcaggt gaaagaaact ctgcaaaaag gcaaaacgcc cagcgaaaaa
    32701 taatgcaata tcgggtgctg accggatatc tttacgccga agtgcccgtt tttccgtctt
    32761 tgtgtcaatg attgttgaca gaaaccttcc tgctatccaa atagtgtcat atcatcatat
    32821 taattgttct tttttcaggt gaaggttccc atgcgtctgg aagtcttttg tgaagaccga
    32881 ctcggtctga cccgcgaatt actcgatcta ctcgtgctaa gaggcattga tttacgcggt
    32941 attgagattg accccattgg gcgaatctac ctcaattttg ctgaactgga gtttgagagt
    33001 ttcagcagtc tgatggccga aatacgccgt attgcaggtg ttaccgatgt gcgtactgtc
    33061 ccgtggatgc cttccgaacg tgagcatctg gcgttgagcg cgttactgga ggcgttgcct
    33121 gaacctgtgc tctctgtcga tatgaaaagc aaagtggata tggcgaaccc ggcgagctgt
    33181 cagctttttg ggcaaaaatt ggatcgcctg cgcaaccata ccgccgcgca attgattaac
    33241 ggctttaatt ttttacgctg gctggaaagc gaaccgcaag attcgcataa cgagcatgtc
    33301 gttattaatg ggcagaattt cctgatggag attacgcctg tttatcttca ggatgaaaat
    33361 gatcaacacg tcctgaccgg tgcggtggtg atgctgcgat caacgattcg tatgggccgt
    33421 cagttgcaaa atgtcgccgc ccaggatgtc agcgccttca gtcaaattgt cgctgtcagc
    33481 ccgaaaatga agcatgttgt cgaacaggcg cagaaactgg cgatgctaag cgcgccactg
    33541 ctgattacgg gtgataccgg caccggaaaa gatctctttg cctacgcctg tcatcaggca
    33601 agccccagag cgagcaaacc ttacctggcg ctgaactgtg cgtctatacc ggaagatgcg
    33661 gtcgagagtg aactgtttgg tcatgctccg gaagggaaga aagggttctt tgagcaggcg
    33721 aacggtggtt cggtgctgtt ggatgaaata ggtgagatgt caccacggat gcaggcgaaa
    33781 ttactgcgtt tccttaatga tggcaccttc cgtcgggttg gcgaagacca tgaggtgcat
    33841 gtcgatgtgc gggtgatttg cgctacgcag aagaatctgg tcgaactggt gcaaaaaggc
    33901 gtgttccgtg aagatctcta ttatcgtctg aacgtgttga cgctcaatct gccgccgcta
    33961 cgtgactgtc cgcaggacat catgccgtta accgagctgt tcgtcgcccg ctttgccgac
    34021 gagcagggcg tgccgcgtcc gaaactggcc gctgatctga atactgtact tacgcgttat
    34081 gcgtggccgg gaaatgtgcg gcagttaaag aacgctatct atcgtgcact gacacaactg
    34141 gacggttatg agctgcgtcc acaggatatt ttgttgccgg attatgacgc cgcaacggta
    34201 gccgtgggcg aagatgcgat ggaaggttcg ctggacgaaa tcaccagccg ttttgaacgc
    34261 tcggtattaa cccagcttta tcgcaattat cccagcacgc gcaaactggc aaaacgtctc
    34321 ggcgtttcac ataccgcgat tgccaataag ttgcgggaat atggtctgag tcagaagaag
    34381 aacgaagagt aagcgtgaat atgcctgatg gtgcaatacc atcaggcata ttaacttatg
    34441 ctttcagtac agccagagct gcttcgtaat ccggctcggt ggtgatttca tccaccagct
    34501 ggctgaaaat cacattgtca ttttcatcaa taaccacaac ggcacgcgct gccagacctt
    34561 tcagtgggcc atcagcaatt gccacaccgt aggcttgcag gaattcagcg ttacggaaag
    34621 tggagagggt gataacgttg ttcagacctt ctgcgccgca gaaacgagac tgggcgaacg
    34681 gcagatcggc agagatacac agcacaacgg tgttgtcgat ctcggttgcc agttggttaa
    34741 acttacgtac tgatgcggcg caaacaccgg tatcaatact cgggaaaatg ttcagcactt
    34801 tgcgtttacc cgcaaactga ccgagggtga cgtcagacag atcttttgcc acgagagtaa
    34861 aagtctgcgc tttgctaccc gcctgcggga tggaattggc gactgtaacc gggttgccct
    34921 ggaaatgaac ggtttgtgac atgattatct ttcctgttta catatagtta acgtcacacc
    34981 tagtttatgc taactgtcaa taacacagca aacgctattt gcgcttaatc cgcagaccac
    35041 cgcgacaaca aggagtaaag atgagaaccg ttaaggtatt tgaggaggcc tggcccttac
    35101 ataccccgtt tgtgattgct cggggaagtc gcagtgaagc gcgcgtggtg gtggttgaac
    35161 tggaagaaga gggtatcaaa ggcaccggtg aatgcacgcc gtatccgcgt tatggggaaa
    35221 gcgatgcctc ggtaatggcg caaattatga gcgtcgtgcc gcaactagag aaagggctga
    35281 cacgggagga gttgcaaaaa atcctccctg ccggcgcagc acgtaatgcg ctggattgtg
    35341 cattgtggga tctggcggcg cgaaaacagc agcaatcgct ggctgatttg atcggcataa
    35401 cgcttcccga gacagtcacc acggcacaga cgatagtcat cggtacgcct gatcagatgg
    35461 ccaatagtgc atcaacactc tggcaggcag gcgcgaaatt actgaaagtg aagctggata
    35521 accatcttat cagtgagcgg atggtggcaa ttcgcacagc tgtgcccgat gcgacgctga
    35581 tcgttgatgc aaatgaatcc tggcgtgcag aagggttggc ggcgcgttgc cagctattgg
    35641 cggatttagg cgttgcgatg cttgaacaac cgcttcctgc gcaggacgat gcggcgctgg
    35701 agaattttat tcatccgtta ccgatttgtg ctgatgaaag ttgtcatact cgtagcaatc
    35761 tgaaggcgct gaaagggcgc tatgagatgg ttaacattaa gcttgataaa accggtggtc
    35821 tgacggaagc gctggcgctg gcgactgaag cgcgtgcaca aggttttcgt ctgatgctgg
    35881 gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca
    35941 gtttcgccga tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt
    36001 tcacgacggg cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag
    36061 atatttttcg ctggcttcgt cggaggagat gggtggaaac tctgcggtga tgcaatgcaa
    36121 gttgagatct gcacaccaac tgccgaaaga gccaggcgtt tcataaccga cgctggttac
    36181 cagaggcaat tcaaacgcct gggccagcca ttcacctaat tcgctgtgtc tgggatcttc
    36241 aatacaggcc agcggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag
    36301 ctggcacagc gcctgggttt caggttcgga gccaggtttg tcacctgtca gtaatacaac
    36361 atcacgttct tcagcggcgc tgttccagcg ataaactgtt tcaccttcct tccagttcgc
    36421 tgccggaaag tttcggttta aatccacacc attagcattg gcccgtaacc ctaactggca
    36481 gccgtcagga ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg
    36541 cagagcacag gagagcgtga cgatcgaaga gttttcatca ccgtgagtgc cagccagaat
    36601 caaaccactt tcgtgactgg ctgcaggggc cggaaaccag attaacggcg cgcccaataa
    36661 tgaacgtccg taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcgagt
    36721 tacggtcatg atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg
    36781 gattatcaaa tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga
    36841 agttgtttgc attttcattg atgtgcagat attagaattg tcgtaatgtt atatacatgg
    36901 cgtattttat tcttactggt tctacacatg ttaatcgcat ctgattgatc agttcacctt
    36961 aataagttta ataaatcgta ctgcacatag tacagtacga tggtagctat ataattgatt
    37021 tacatgaaga agggcttatc accatcagtc ccgggttgat tgatgcctat attctcacca
    37081 atgtgtttct caggtttatc gatgatatct gtgattaatg ctgcgacact cttacgtgat
    37141 acgatcgtac ctttgaatgg ttcattgcgt gaggtaagct cataatcaat aatatcttca
    37201 tcagtcagcc aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc
    37261 gcagccctgc ggaatggttt taggggttca ccaataactg cattattcca ttcgccaaat
    37321 tttcctggaa cttcatcata aataccgagg gaaagaacaa agatcagacg tttaacatca
    37381 caggctttca tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg
    37441 ttggcataca caacatcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca
    37501 tcccccataa taatcttgct attagttgga tagggtttgt ggatttttgc tggctgacgg
    37561 gcaaataaag tttgtttaat ggtttgttta tctgccagtt gattaatcac atggcgggct
    37621 atctgaccgc cagcaccaag aatcagtaca tttttcatga aacttttccc agattattgg
    37681 cagcaatatg ccaggccata tcactattac ggatttgaat atgtttcatt acagagtttc
    37741 ctcaaagaac ggagctaata ctgaaattac ttcagccaca tagactttgc catcataaag
    37801 atccatatgg tttgcgcctt caacaatgtg atagcgttta tcctggcttg atgctcgatc
    37861 gtacaggtcg tcactcatcc atttgctccc agccaggctg cccgccacaa tctgtatcgg
    37921 ctgagtcagg tacacttccg ccatatggta agcatcatag gtaataatct ggttaaggct
    37981 gcgcaaagta gcgtaacccg gtgctgttgg atactgcgcg cgaggggtgt ggtaatattc
    38041 ccaggcctga cgcagttctt cgttcggcgc atcggactcc ttcattggtg ccagtggcat
    38101 agtggcgtat tctccgctgc taatatcgct ggttcgggcg tttgaacccg cgtcaacgta
    38161 tggtagggca tcaatagatt tcacattgtt ttcccaacca ttacggaaca tcgaaccaat
    38221 attgaccgca ctaacagtac cgatggcctt gatgcggcga tcctgaattg cagcattggc
    38281 tgtatatcct gcaccggcac aaattcccat cgcaccaatt cgggtattgt cgacataaga
    38341 aagcgttgtc aggtaatcaa tcacggcact gacgtcttca gtacgaatgt atgggttttc
    38401 taactgacgc ggctcgccgc cgctttcacc ttgataagat gcgtcataag caatagtgac
    38461 aaatcctttt tccgccagtt tttcggcata ggttccggcc gtttgctctt taacgccccc
    38521 acctgggtga gataccacaa ttgccggata ctgacgggtt tcatcaaatt ttggagggaa
    38581 atagatcact gcagacaaag agatggttgg attattgctg ttagtgaagc tgactttatt
    38641 attcatcatt ccgttcctca tggagttgtc ggttcgtttt aacggttggt gatatcacta
    38701 tagatattga tcattaagtt gattagaccc aaaatcatga ttagacctat catttaaatg
    38761 attaatagat tgtatggaga tactgaatga agcgtgaaga aatcgctgat ctgatggcgt
    38821 ttgtcgttgt tgcagaggag cgtagcttca ctcgcgcagc agcccgcctg agcatggcgc
    38881 agtcagcttt aagccagata gtgcgccgta tagaagaacg attgggattg cggcttctga
    38941 cgcgaaccac gcgcagcgtt gtacccactg aagcgggcga gcatcttttg tctgttcttg
    39001 gccctatgtt gcatgacata gattctgccc tgacatccct gagcgatctg cagaaccgcc
    39061 catccgggac aatacgtatt actactgtag aacatgcagc aaaaacgata ttgttaccag
    39121 ccatgcgcac attcctgaaa tcgcatcctg aaattgatat tcagcttacc attgattatg
    39181 gtttgaccga tgtcgtttct gaacgttttg atgcaggcgt ccgtctgggt ggggagatgg
    39241 ataaagatat gatcgccatt cgaatcgggc cagacatacc aatggctatt gttggctcac
    39301 cggattattt ttctcgtcga agtgctccaa cgtcagtgtc acaattaata gatcatcagg
    39361 caattaattt gtatcttccc acatcgggta cagcaaatcg ctggagatta atacgcggtg
    39421 gacgtgaagt tcgtgttcgc atggaaggtc agcttttact gaatacgata gatctgatca
    39481 ttgatgctgc aattgatggg catggattgg cgtatctacc ttatgatcag gtggagcggg
    39541 ctattaaaga aaaaaaactg atacgtgttt tggataaatt cacaccagat ttacccggtt
    39601 atcacctgta ctatccacac cgtcgacatg ctggctcggc attctcatta tttatagata
    39661 ggctgaagta taaaggtgct gtttagcact acttgctgat acattaattt aattcttctc
    39721 ttaacgtatt ctcagttcct ttcaacgttt tggttatttt ttattcttcg cacaatggcg
    39781 acagatgctg attatgatac cgaaaacggg tttgaatgtg cgaagcccga acgtagtgtt
    39841 cggagttcta tgtgctttac cgcattttgg agactattat ttacactaaa tctgatttga
    39901 tatattgata cttaaaacat ttgatgcttc ctttgtcact tttttgatgg aagttgtttg
    39961 catttcttta aggcgaaaca aataattacg catcaatttt aatgtcggtt agagggaaac
    40021 ttatgaagca ctctgtttca gtcacgtgtt gtgcgctgtt ggtcagcagc atttctcttt
    40081 cgtatgctgc tgaagttccg agcggcacag tactggcaga gaagcaggag ctggtgcgcc
    40141 acattaaaga tgagccagcg tcgctggatc ccgctaaagc cgtgggcctg ccggagattc
    40201 aggtcattcg cgatctgttt gaaggactgg tgaatcagaa cgagaaaggg gagattgtcc
    40261 ccggcgttgc gactcagtgg aaaagtaatg acaaccgtat ctggactttt accctgcgcg
    40321 ataacgcaaa atgggcggat ggcacaccgg taacggcgca agattttgtc tacagctggc
    40381 aacgtctggt ggacccgaaa acgctttcgc catttgcatg gtttgccgcg ctggcgggaa
    40441 tcaacaacgc acaggcgatt attgatggta aagccacgcc tgaccagctt ggcgttaccg
    40501 cagttgatgc ccatactttg aaaatccagc ttgataaacc gttgccgtgg tttgtgaatt
    40561 taaccgctaa ctttgccttc ttcccggtgc aaaaagccaa cgtagaaagc agtaaagagt
    40621 ggacgaaacc cggaaatctg atcggcaatg gcgcttatgt tcttaaagag cgcgtagtca
    40681 atgaaaaact ggtcgtagtg ccgaataccc attattggga taacgccaaa acggtactgc
    40741 aaaaagtgac cttcctgcca attaatcagg aatccgcagc cactaagcgt tatctcgcgg
    40801 gggatattga tatcaccgaa tccttcccga aaaatatgta tcagaagctg ttgaaggata
    40861 ttccggggca ggtttatacg ccgccgcagc tcgggaccta ttattatgcg tttaacacgc
    40921 aaaaagggcc gacggcagat cagcgcgttc gcctggcatt aagtatgacg atagatcgcc
    40981 gcctgatgac cgaaaaagtg ttggggacgg gcgaaaagcc agcgtggcat tttacaccag
    41041 atgttaccgc gggatttacg ccggaacctt cgccgtttga acaaatgagt caggaagaac
    41101 tgaatgcgca ggcaaaaact ttgttgagcg ccgctggtta tggtccgcaa aaaccgctga
    41161 agctgacgct tttgtataac acttcagaaa accatcaaaa aattgcgatt gctgtagcat
    41221 cgatgtggaa aaagaacctg ggcgtagatg ttaaattgca aaaccaggaa tggaaaacct
    41281 atatcgatag ccgtaatacc ggcaattttg atgttatccg cgcctcgtgg gtaggggatt
    41341 ataatgaacc ctccactttc ctgacattat taacgtcaac gcattccggg aatatttcac
    41401 gctttaacaa tccggcatat gacaaagttc tggcccaggc atcgacggaa aataccgtta
    41461 aagcgcgtaa tgccgattac aacgcggcag aaaaaatcct catggagcaa gcaccgattg
    41521 caccaattta tcaatatacc aatggacgat taatcaagcc gtggctgaaa ggttatccca
    41581 ttaacaaccc tgaagatgtg gcgtacagtc ggactatgta tattgtgaag cattgatgtg
    41641 atgagaactg gcgttaccct tgtggataac gccagtgatg tctgatttag cgaccctggt
    41701 ccgccggtgt aatgttatcc atatacagcg tctggctggg gaaggcaaag tccgcgccgt
    41761 gtgactgtac aatatcgata atcttcaaat aaacgtcttg ctgtgcagca agccattcag
    41821 cccataccgt ggttttggta aagcaataaa ccataatatt taatgacgaa tcagcaaact
    41881 ggttgaaata aaccagtaag gtttgtcgct ggtcgatggc cgggtgattt ttcagcatct
    41941 cacgtacggc ttcgacaata acgcccactt ttgccgcatc ctcataacgt aaaccaatgg
    42001 tcgtggtaat gcgccggttg gtcattcgtc caggattttc tacgctgatc gacgaaaaca
    42061 gcgagttcgg tacgtacaat ggacgattat caaaggtctt aattttggta attcgccagc
    42121 caatttccgc tactgtacct tcgatatttc tgtccggtga acggatccag tcgccaatac
    42181 tgaaaggacg gtcgaaatag agcataatcc cggaaaagaa gttactcaga atatctttac
    42241 cggccatacc gacagccaga ccaccaatac caccaaaggt caacaagcca gaaaggctca
    42301 tgccgaaatg ttcgccataa agcagaacaa gcaccacaat aatggtgatt ttgataatgc
    42361 gcgacatgat ccgcgcactg gtgatatcgc gacctttttt aatctgctgt ttttcaaact
    42421 gattaatcag cagaaatagc ttaatcgtca gaataaccgc aatcagggac gtacagataa
    42481 aatcgataac gcctggggtg ataaatttga gtttatagtt ttctataaca tagttaatga
    42541 tgctaccaac agcactaata attatggtgt agattaaaaa ttgcaccgca tggaataaaa
    42601 atccttttct tttacgattt ccacggcgaa accaaaagct catcagaatc aatgctgcgc
    42661 agctaccgaa aataatgacc agattaagcg cattatttgt aaacagttca gcgatcattg
    42721 ttttatcagg ctcctccaga taattgtcgt catgccggaa acccctggcg ggactatttt
    42781 accgcgacaa ttcattcaga tcatcaatag tcagggaagg aagtagcaac attagctaac
    42841 taatcaatgc gttgattgta aatccagcta agaggtgagg ttttcagagc agacaacggt
    42901 gaaatgtcat ggtattgtta cgtttaggta acaaaaaatt tgtctgcaca aggattacat
    42961 catgattatg gcgaaactga agtcagcgaa agggaagaaa tttctctttg gtttgttggc
    43021 ggttttcatt attgcggcgt cggttgtgac tcgcgcgacc atcggcggcg ttatagaaca
    43081 gtacaatatt ccgctgtctg agtggacgac atcaatgtat gtgattcagt catcgatgat
    43141 ttttgtttat agcctggtct ttactgtgtt gctggcaatc ccgttgggaa tttatttcct
    43201 tggcggcgaa gagcagtaag taaaaaatag gcccgataac tcgggccttg tcagttattg
    43261 aagagtcgtt aatcgtcttc ttcgtcatcc agttcaacgg gtgtctgata ctggtcaggt
    43321 ttaatgacca gcaggtcgca gcgaagatga tcaatcacct gttccgccgt gttgccgagg
    43381 aatgctgctg aaataccggt gcgtccgacc gtgcccagaa ccacaatccc cgcctgtaag
    43441 tgctccgcca aatcaggaat cacctcttct ggcagacctt tttctacgtg cgtcatgttt
    43501 tcattaatgc cgaatttctg tcgcagagct ttcattgcca gcaaatgttg cccacgaatg
    43561 gcatcgttat aaacgctcgg gtcaaattcc ggcagttcaa tcgcgatatt aattggcgtt
    43621 accggataag cgccaaccag atgaacttcg gtatggttga cttgttctgc cagttcgatc
    43681 gtctctttga ccagtttttc attgagcgca ttatggtacg gctcttcact ggcgagattc
    43741 accgccacca gcgccttacc cccttccggc cacggctggt ctttcaccat ccacacgggg
    43801 cttgggcatt tgcgtaacag atgccagtcc gttggcgtaa aaatcaccgc ttccagacgg
    43861 tcatgttggt gcgccatttt tagcaccaaa tcgtgcccgc cgctgatcac ttcctgaatg
    43921 atggcttcga aaggacggtt atgccagacc actttaattt caatgggaac gccagcattg
    43981 agataatatt ttgcctgctc gtggatccag gctgtacgct ggctgatgac gccctgacgc
    44041 atagcggtac gttcgtccgg agagagcagg gtggtcattt cgtatgagaa gtcatagatc
    44101 ggcaaaaagg ctttaatttt gccaccaatc cgttgatgta aataaacagc tcgccgcaat
    44161 gctggttggt cgtcctggtt aggatcgata acaacgagca tgttctgata catagccata
    44221 cagggtctcc ttacaacaac tgtcaacgca gtttgtaatt aaaagattaa cccatatctg
    44281 gtgaatgaaa cagtgatgaa ccttctgtca gatcaataaa tcagaaaaat ttaatgatat
    44341 gacagaagga tagtgagtta tgcggaagaa tcaggcaacg ttacgcgtgt gaccagcaag
    44401 ctgggccagc gcatcgttat tttcgatagt gatgtattta cctttgactg ccagcatacc
    44461 gcttttctgg aagcgaccca gcagacggct gatggtttca accgtcaggc ccagatagtt
    44521 accgatatca ccacgagtca tcgtcaggcg gaattcacga ggggagaagc cgcgttgggc
    44581 aaaacgacgg gacaggttgt agatgaatgc agccagacgt tcctcggcat ttttcttcga
    44641 caacagcagg atcatgtcct gatcgccttt gatttcaccg ctcatcagac gcatcatctg
    44701 ctgacgcaga ttcggcattt taccagacaa atcgtccagc gtttcgaacg ggatttcaca
    44761 taccatcgag gtttccagcg cctgcgcgaa actcgggtga tgaccgctgc cgatggcatc
    44821 aaatcccacc agatcgcctg ctaaatggaa accagtgatt tgctcgtcgc cttgctcagt
    44881 gatggtataa cttttaatcg taccggagcg gatggcataa agcgatttaa gttcatctcc
    44941 agccttaaac agcgtctggc ctttctgaat aggcttcttc cgctcaatga tattatcaag
    45001 ctgatcaagc tcatgttcgt tgagtgtgaa cgggatgcaa agctggctga tgctgcaatc
    45061 ctggcaatgg atagcacaac cgccagactg aatgcgccgt ataattcgct tttccgggat
    45121 cataggtctg ctcaagccgt aattgatatt tgtcaatttt aacatctttt tagggagcaa
    45181 gtaagtctaa gcaaacctta acagcagaga attccgatat tagatgtaaa tatatgtcta
    45241 tctatttgaa aacccttaag ttgttaaggg tgactttaca taaaagtgtg aacaagctgg
    45301 cacaaattgt ttaatgttta cagcaaaaga taaccttcgt ggcgcaataa ccactctttt
    45361 cgctgaactc cgcctgcata tccggtcatg gtgccgtttc ggccaataac ccgatggcaa
    45421 ggtacgacga tgctgatggg attcgatccg tttgccgcac caacggcacg cgccgcgcca
    45481 gggcggccta attgttcagc cagttggcca taatgcatta cctgcccgca ggggatagtg
    45541 cgcagtgttt tccagacttc gcgctgaaat ggcgtccccc ccgtggcagt gggaagcgta
    45601 tcaataatgc taagattacc ggcaaaatat tcacgcagct tgtcgcttaa accacctggg
    45661 ttggtggcag aaatgcgctc atagccttct ttgcgataat ggatgtccag cagctgcacc
    45721 atgcgttcgc tgtactcttc ccattcaacc gcccgcaggc gaaattgctc atcgcaaatc
    45781 acccacagtg gacccagtgg cgtggcaatt ttttcttcaa gtaatctcag catccgttct
    45841 ctcttaagac aaacgtgggt aaatacccgg acctatcggc aagccaacaa gataccacgc
    45901 cagcaacatc agcagccata ccaccaaaaa gataagtgga tagggtaaga ctaacgaata
    45961 gtaagtaccc agtttcgcgt ctggtttgtc gcagtctgat agtattaagc gattttttga
    46021 tttttctgcc tgaagaaatg agggcaagcc atcacatgga tcgagggtgg gataattcca
    46081 gtcagcgaaa ttttaccgta ggttatctgt tatttgatct gtggcgtgat agccgccaga
    46141 caccactgat ctcgtcactc attccccaat cccggcgaga atgtggttat ttacagggct
    46201 aaaaaatgaa taaaaaaata gagcctgtcg acatccgcca gactctacag tacacacagc
    46261 agtgcatcct cgtcttaaat ccggcctggg gtcctggtga ccggtgacag tgttataacg
    46321 gttttaccca tccgttgcca attttcttca gtgacagatt tcacgaaaat ataagttaaa
    46381 tcatttgtct tgtgattgca taatcatgca tcgttaatcg acgagtaaat ttaccgggcg
    46441 agtagcccgt tcgacgctta aacatattgc cgaacgattc agctgaggca tagcccagac
    46501 gatgggcaat tttggtcaac gaatatccat catcaagaag cgtcactgca gaaataacgt
    46561 gcatttgctg aacccagtgg cgaaatgtta atcccgtctc tttaacaaat aagcgcatga
    46621 gtgtacggct actgacgtta attttatccg ctagattatc cagtgttggc agataccctt
    46681 ggttgtttag caaattctca caaagctgct gtagccgctc atcatttggc caagggagtt
    46741 caagtgacat tttcaccccc atttttattt cctgaagtat cagtgtctcg agggctgaaa
    46801 aacgtaactg ttgtttatct gtggtacttt gcgacggtgg aagttggtta attgcaatta
    46861 gtaattccct taacaggggt gaaatacccg tcatgaagca aacttttccc agcgtttcac
    46921 tttccgcttc tgaaaccagt acggttttta aaagtacgtt gcctgtcgca acaaacttat
    46981 gcggcatttg tgggggcagc caaagcgcgc tgctggcatg cactagccag acgctttctc
    47041 cagcccatac acgaacgact ccttcagccg ggtaaagcaa ctgggcatag agatgtttat
    47101 gccagggaac tgtacctcca ttcagatgtt ccgaatccag tccgataatg tgatgccctg
    47161 gtgatgcttt gggttgcagt gtgattgtca tttacagtag gtattttgtc agaaatcatc
    47221 aaataagcca tgttaaattt taaatatgac gtatgcaagt ttccggatat atacggtgta
    47281 aagagtgatg ataatttcta aaaaacagct t
//
LOCUS       DAOFSV010000023        47170 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.023, whole genome shotgun sequence.
ACCESSION   DAOFSV010000023 DAOFSV010000000
VERSION     DAOFSV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.023"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..421)
                     /gene="rplI"
                     /locus_tag="QBH27_001383"
     CDS             complement(<1..421)
                     /gene="rplI"
                     /locus_tag="QBH27_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDQ7013714.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFA"
     gene            complement(463..690)
                     /gene="rpsR"
                     /locus_tag="QBH27_001384"
     CDS             complement(463..690)
                     /gene="rpsR"
                     /locus_tag="QBH27_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDQ7013715.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(695..1009)
                     /gene="priB"
                     /locus_tag="QBH27_001385"
     CDS             complement(695..1009)
                     /gene="priB"
                     /locus_tag="QBH27_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDQ7013716.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1016..1411)
                     /gene="rpsF"
                     /locus_tag="QBH27_001386"
     CDS             complement(1016..1411)
                     /gene="rpsF"
                     /locus_tag="QBH27_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDQ7013717.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            1739..2014
                     /locus_tag="QBH27_001387"
     CDS             1739..2014
                     /locus_tag="QBH27_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7013718.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2143..2829)
                     /gene="ulaF"
                     /locus_tag="QBH27_001388"
     CDS             complement(2143..2829)
                     /gene="ulaF"
                     /locus_tag="QBH27_001388"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDQ7013719.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFVWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(2829..3683)
                     /gene="ulaE"
                     /locus_tag="QBH27_001389"
     CDS             complement(2829..3683)
                     /gene="ulaE"
                     /locus_tag="QBH27_001389"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDQ7013720.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMT
                     KAGMVEAV"
     gene            complement(3693..4343)
                     /gene="ulaD"
                     /locus_tag="QBH27_001390"
     CDS             complement(3693..4343)
                     /gene="ulaD"
                     /locus_tag="QBH27_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDQ7013721.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(4357..4821)
                     /gene="ulaC"
                     /locus_tag="QBH27_001391"
     CDS             complement(4357..4821)
                     /gene="ulaC"
                     /locus_tag="QBH27_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDQ7013722.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(4831..5136)
                     /gene="ulaB"
                     /locus_tag="QBH27_001392"
     CDS             complement(4831..5136)
                     /gene="ulaB"
                     /locus_tag="QBH27_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDQ7013723.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(5152..6549)
                     /gene="ulaA"
                     /locus_tag="QBH27_001393"
     CDS             complement(5152..6549)
                     /gene="ulaA"
                     /locus_tag="QBH27_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDQ7013724.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFASRAL
                     RAEEDAEKQLAEQSA"
     gene            6904..7968
                     /gene="ulaG"
                     /locus_tag="QBH27_001394"
     CDS             6904..7968
                     /gene="ulaG"
                     /locus_tag="QBH27_001394"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDQ7013725.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8076..8831
                     /gene="ulaR"
                     /locus_tag="QBH27_001395"
     CDS             8076..8831
                     /gene="ulaR"
                     /locus_tag="QBH27_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDQ7013726.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8828..9577)
                     /gene="yjfP"
                     /locus_tag="QBH27_001396"
     CDS             complement(8828..9577)
                     /gene="yjfP"
                     /locus_tag="QBH27_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ7013727.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLTELGNRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            9759..10088
                     /gene="bsmA"
                     /locus_tag="QBH27_001397"
     CDS             9759..10088
                     /gene="bsmA"
                     /locus_tag="QBH27_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDQ7013728.1"
                     /translation="MVSRKRNSIIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10237..10512
                     /gene="yjfN"
                     /locus_tag="QBH27_001398"
     CDS             10237..10512
                     /gene="yjfN"
                     /locus_tag="QBH27_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDQ7013729.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(10629..12254)
                     /locus_tag="QBH27_001399"
     CDS             complement(10629..12254)
                     /locus_tag="QBH27_001399"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDQ7013730.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     TWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(12338..13501)
                     /locus_tag="QBH27_001400"
     CDS             complement(12338..13501)
                     /locus_tag="QBH27_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDQ7013731.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(13504..14142)
                     /locus_tag="QBH27_001401"
     CDS             complement(13504..14142)
                     /locus_tag="QBH27_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDQ7013732.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(14152..14550)
                     /locus_tag="QBH27_001402"
     CDS             complement(14152..14550)
                     /locus_tag="QBH27_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDQ7013733.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(14568..15227)
                     /locus_tag="QBH27_001403"
     CDS             complement(14568..15227)
                     /locus_tag="QBH27_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDQ7013734.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLSFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(15277..15975)
                     /locus_tag="QBH27_001404"
     CDS             complement(15277..15975)
                     /locus_tag="QBH27_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDQ7013735.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(15994..16395)
                     /locus_tag="QBH27_001405"
     CDS             complement(15994..16395)
                     /locus_tag="QBH27_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDQ7013736.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNNESALIIKLNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(16522..17253)
                     /gene="rlmB"
                     /locus_tag="QBH27_001406"
     CDS             complement(16522..17253)
                     /gene="rlmB"
                     /locus_tag="QBH27_001406"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDQ7013737.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(17344..19785)
                     /gene="rnr"
                     /locus_tag="QBH27_001407"
     CDS             complement(17344..19785)
                     /gene="rnr"
                     /locus_tag="QBH27_001407"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDQ7013738.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(19824..20249)
                     /gene="nsrR"
                     /locus_tag="QBH27_001408"
     CDS             complement(19824..20249)
                     /gene="nsrR"
                     /locus_tag="QBH27_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDQ7013739.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(20454..21752)
                     /gene="purA"
                     /locus_tag="QBH27_001409"
     CDS             complement(20454..21752)
                     /gene="purA"
                     /locus_tag="QBH27_001409"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDQ7013740.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGLEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(21856..22053)
                     /locus_tag="QBH27_001410"
     CDS             complement(21856..22053)
                     /locus_tag="QBH27_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDQ7013741.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(22135..23139)
                     /gene="hflC"
                     /locus_tag="QBH27_001411"
     CDS             complement(22135..23139)
                     /gene="hflC"
                     /locus_tag="QBH27_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDQ7013742.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(23142..24401)
                     /gene="hflK"
                     /locus_tag="QBH27_001412"
     CDS             complement(23142..24401)
                     /gene="hflK"
                     /locus_tag="QBH27_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDQ7013743.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(24487..25767)
                     /gene="hflX"
                     /locus_tag="QBH27_001413"
     CDS             complement(24487..25767)
                     /gene="hflX"
                     /locus_tag="QBH27_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDQ7013744.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(25843..26151)
                     /gene="hfq"
                     /locus_tag="QBH27_001414"
     CDS             complement(25843..26151)
                     /gene="hfq"
                     /locus_tag="QBH27_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDQ7013745.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(26237..27187)
                     /gene="miaA"
                     /locus_tag="QBH27_001415"
     CDS             complement(26237..27187)
                     /gene="miaA"
                     /locus_tag="QBH27_001415"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDQ7013746.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(27180..29027)
                     /gene="mutL"
                     /locus_tag="QBH27_001416"
     CDS             complement(27180..29027)
                     /gene="mutL"
                     /locus_tag="QBH27_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDQ7013747.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRNMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPSSGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(29037..30368)
                     /gene="amiB"
                     /locus_tag="QBH27_001417"
     CDS             complement(29037..30368)
                     /gene="amiB"
                     /locus_tag="QBH27_001417"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDQ7013748.1"
                     /translation="MMYRIRNWLVATLLLLCAQAGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(30387..30848)
                     /gene="tsaE"
                     /locus_tag="QBH27_001418"
     CDS             complement(30387..30848)
                     /gene="tsaE"
                     /locus_tag="QBH27_001418"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDQ7013749.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(30820..32367)
                     /gene="nnr"
                     /locus_tag="QBH27_001419"
     CDS             complement(30820..32367)
                     /gene="nnr"
                     /locus_tag="QBH27_001419"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDQ7013750.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLR
                     LDRWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMGSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            32366..33505
                     /gene="queG"
                     /locus_tag="QBH27_001420"
     CDS             32366..33505
                     /gene="queG"
                     /locus_tag="QBH27_001420"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDQ7013751.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGEIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(33488..33541)
                     /locus_tag="QBH27_001421"
     CDS             complement(33488..33541)
                     /locus_tag="QBH27_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013752.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(33775..33850)
                     /locus_tag="QBH27_001422"
     tRNA            complement(33775..33850)
                     /locus_tag="QBH27_001422"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33815..33817),aa:Gly,seq:gcc)
     gene            complement(33886..33961)
                     /locus_tag="QBH27_001423"
     tRNA            complement(33886..33961)
                     /locus_tag="QBH27_001423"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33926..33928),aa:Gly,seq:gcc)
     gene            complement(33998..34073)
                     /locus_tag="QBH27_001424"
     tRNA            complement(33998..34073)
                     /locus_tag="QBH27_001424"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34038..34040),aa:Gly,seq:gcc)
     gene            complement(34284..34829)
                     /gene="orn"
                     /locus_tag="QBH27_001425"
     CDS             complement(34284..34829)
                     /gene="orn"
                     /locus_tag="QBH27_001425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDQ7013753.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            34924..35976
                     /gene="rsgA"
                     /locus_tag="QBH27_001426"
     CDS             34924..35976
                     /gene="rsgA"
                     /locus_tag="QBH27_001426"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDQ7013754.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            36073..37041
                     /gene="psd"
                     /locus_tag="QBH27_001427"
     CDS             36073..37041
                     /gene="psd"
                     /locus_tag="QBH27_001427"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDQ7013755.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            37063..40386
                     /gene="mscM"
                     /locus_tag="QBH27_001428"
     CDS             37063..40386
                     /gene="mscM"
                     /locus_tag="QBH27_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDQ7013756.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(40417..40731)
                     /locus_tag="QBH27_001429"
     CDS             complement(40417..40731)
                     /locus_tag="QBH27_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDQ7013757.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(40829..42331)
                     /gene="yjeM"
                     /locus_tag="QBH27_001430"
     CDS             complement(40829..42331)
                     /gene="yjeM"
                     /locus_tag="QBH27_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDQ7013758.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(42550..43527)
                     /gene="epmA"
                     /locus_tag="QBH27_001431"
     CDS             complement(42550..43527)
                     /gene="epmA"
                     /locus_tag="QBH27_001431"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDQ7013759.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            43852..45660
                     /gene="frdA"
                     /locus_tag="QBH27_001432"
     CDS             43852..45660
                     /gene="frdA"
                     /locus_tag="QBH27_001432"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDQ7013760.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            45653..46387
                     /gene="frdB"
                     /locus_tag="QBH27_001433"
     CDS             45653..46387
                     /gene="frdB"
                     /locus_tag="QBH27_001433"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDQ7013761.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            46398..46793
                     /gene="frdC"
                     /locus_tag="QBH27_001434"
     CDS             46398..46793
                     /gene="frdC"
                     /locus_tag="QBH27_001434"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDQ7013762.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            46804..47163
                     /gene="frdD"
                     /locus_tag="QBH27_001435"
     CDS             46804..47163
                     /gene="frdD"
                     /locus_tag="QBH27_001435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDQ7013763.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
BASE COUNT        11413 a        12907 c        11773 g        11077 t
ORIGIN      
        1 tcgcgaatac ttcgctgtga acctggaagc tcacttcgtg ttcaccagtg gtacgcagaa
       61 cgccgttcgg cagacgaact tcgctcttag ccacttcaac gccagctgca gttacagcgt
      121 cagcgatgtc gcgagtaccg atggaaccga acagtttacc ttcgtcgcca gctttagacg
      181 cgatggtaac agtttccagt gcattgattt tctcagcgcg agcattagct gctgccagaa
      241 cttcagccag tttagcttcc agttcagcgc gacgtgcttc gaagaattca atgtttttct
      301 tggtagctgg aacagcttta ccctgcggta ccaggaagtt acgagcatag cccgctttaa
      361 cgtttacctg atcacccagg ctacccaggt ttgctacttt atcaagcaga ataacttgca
      421 ttaccttatc ctctcaaagt cgtattaatg gaccgtgacc gattactgat ggcgatcagt
      481 gtacggcagc agggacaggt agcgagcgcg tttgatagcg cgagccagct gacgctggta
      541 ttttgcacgg gtaccggtga tacggcttgg gacaatctta ccgctttcgg tgatgtagtt
      601 tttcagcgta gcgatatctt tatagtcgat ctcttgaacg ccttccgcgg tgaaacggca
      661 gaacttgcga cgacggaaat aacgtgccat atggctagtc tccagaatct atcaattcaa
      721 tctgctcggc atgcaaaacc attttgctca gtccgttctt tgccttatgg catgaaatga
      781 acccctgaac ggttatgcga ctgccgaccg ttatactgtg agtaatggcc tggttttcgt
      841 gtccgctaac aataacgggc atttggcacc acgcctgccg gtgaaagccg gcttcctcct
      901 gcacagaacg atgctcaagc acgaactggc agtgaggaat tcctgatgga ctgacctttc
      961 gaaggggagt cctgcacacg gtgccggaca acaccagacg gttggtcatc agaaattact
     1021 cttcagaatc cccagcttca gcatcatcag cggtttcgtt tgcgaaatca tcgcgacgct
     1081 cacggcgctc gtctttcgct ttaaccatcg gagatgcttc ggtaacagcg tgcttggtac
     1141 gcataaccat gctgcggata acggcatcgt tgaagcggaa ggtagtttcc agctcatcga
     1201 tcacttcctg cggagcttca acgttcatca gaacgtagtg tgctttgtgc agtttgttga
     1261 tcgggtaagc cagctgacgg cggccccagt cttccagacg gtggatcttg ccttctgcac
     1321 cagtgatggc agcagtgtag cgctcgatca tgcccggaac ctgttcgctc tggtcaggat
     1381 ggaccataaa aacgatttcg taatgacgca tcgaattgct ccttacggat tattcagcct
     1441 cctgtctggg tcagccgaat cccggggagg caaggaacgt gttaaaggtc ggctgaaaaa
     1501 tgacgcgtta tagtactgac ataccccgct aaactcaagg gctgagcaga aataaatcgc
     1561 ccctttcgca aataacctct aaatcgctga tttggagttc atcacacttc gtcatggtgt
     1621 ttttttgaac aactgcatca gaaatggtca ccatcaactt ctttatcatt gattaaaatt
     1681 aaatcagaag atcaccacaa gaggagagca cacaaagtca gccgtcgagg aggtatctat
     1741 gttcagtcgt gttttagccc ttctggctgt gcttttgcta agtgcaaata catgggcagc
     1801 cattgaaatt aataaccacc aggccagaaa tatggatgat gtgcaaagct taggtgtgat
     1861 ttatatcaac cataacttcg ccactgaaag tgaagcacgt caggcattaa atgaagagac
     1921 agatgcgcaa ggcgcaacgt actaccatgt gattctgatg cgggaaccgg ggagtaacgg
     1981 caatatgcac gccagcgcgg atatttatcg ctagcaccag gtataaccaa cgaaacattg
     2041 ccatagtttg ctttgccccc ttcgcagggg gctttttttg aaaaacggct gcggaattag
     2101 accagttatc tcccgaagaa ggaaatttcc gcagcgcgtg ttttacttct gcccgtaata
     2161 ggcgttggga ccgtgcttac gcatgaagtg tttattcatc agaaagctgt cgatgtgatt
     2221 cagttgtggg ttaatactgc gcgcaatcca cgccattttc gccacttctt ccatcaccac
     2281 cgcgttatgc accgcatcgt gggcatcttt cccccagacg aacggtccgt gctgatacac
     2341 cacaattccc ggcgtatgca gcggctcggc gtcacccagc gtttcgataa tcactttgcc
     2401 ggtattcagt tcgtactcac cctgcacctc ttcttcgctt aatccgcgcg tgcacgggat
     2461 gtcgccaaag aagtaatcgg cgtgcgtggt gcctaacgcc gggatcgcca gccccgcctg
     2521 cgcccatgcg gtggcatgag tggagtgggt atggacaata ccaccaagcg acggataacg
     2581 acggtagagt tcgagatgcg tcgcagtgtc ggaagacgga cgatactccc cttccaccac
     2641 cttaccgctc atatcgacca ccaccatatc gtctgctttc atggtttcgt aggcgacgcc
     2701 gctgggcttg atcaccacca gcccgcgttc gcggtcaatg gcgctgacgt tgccccaggt
     2761 aaaggtcacc agcccgtaac gcggcagatc catgttggcc tcaaacacct gctgtttcag
     2821 cttttgcatt ataccgcctc caccatgcct gctttggtca tgcgcgcttt cacccaatcg
     2881 cgcgctttcg ccacttctgc cgccggatct tccgccgttt cgctccacat ctcaatcagg
     2941 tacggcccgc aatagccact ctgtttgagc gtttcgaagc aacgttcaaa atccacaaca
     3001 ccttcgccaa aaggcacgtt tttgaagacg cctggtttgg tgtctttcac atgtaccgcg
     3061 acgatatgcc cgattccggc ctgcaactcc atctgcacgt cgttgtccca cgccgacagg
     3121 ttgccgatat ccgggtagag ctggaaccac ggattgttga gatagtgtgc gtagcccagc
     3181 gccttgctga tggagttcat caatgggtaa tccatgatct ccatcgccag cgtgacctgc
     3241 gcgcggcttg ccatctcaac gctctctttc aggccgtcac gaaaacgacg acgcgtttcg
     3301 ttattggctt cctgatagta aacgtcatag cccgccagtt ggatcacgcg aatgccgacg
     3361 tcctgggcga actggatagc tttacgcata atctccagcc cctgcgcccg caccgcgtca
     3421 tcttcgcttc ccagcgggaa acgacgatga gcagaaaggc acatggacgg tacgcgcacg
     3481 ccggtttcaa caatcgcgtt caccagtgcc agacgctgct cgcggctcca gtcgaggcgc
     3541 gaaaggcgat cgtccgtttc atctactgac atctcgacaa aatcgaagcc taacgttttt
     3601 gccagttgca gtcgttccag ccagcactcc ccagcgggga gcgctttttc gtagatgcca
     3661 agcgggattt gtttggacaa catatccgct ccttagcccc acagttcggc gatggaacgt
     3721 ttgaactgac gcgcggcttc aaccggagag gctgcatcac ggatgctgcg accagcgata
     3781 aacacatgaa tcggaatgcc tttaaacaac ggcagatctt ccagcgccag accaccggtg
     3841 acggtgactt tgaagcccat atcggaaaga cgtttgatcg cggtaatgtc cgcttcgccc
     3901 cacgccacgc ctgcggcctg cgcgtcacgg ctgcggtgat aaaccacctg cccaatgcct
     3961 gcgtcgcgcc actgctgcgc ctgttcccag gtccagtagc cggtcagttc gatctgcacg
     4021 tcgccattaa actcttttgc cacgtccagc gcgcctttgg cggtgttgat atccgcacaa
     4081 caaattacgg tcacccagtc agcgttggct tcgaagcaca tgcgagaaag gattttgcct
     4141 gcatcggcaa ttttggcgtc agccagtacg attttgtgcg ggtacagcgc tttcaggtca
     4201 cgaaccgcac gcacgccttc gcctacgcaa agaatggtgc ccacttcgat aatgtcgact
     4261 tcttcggcaa tcaggcgagt ggtttcgtag gcgctatcca tagtctggtt gtccagcgcg
     4321 acttgcaaca tcggtaatga catgttcaat tccttcttaa gctgccgcgt tggtgcggtc
     4381 aatgagatcc agtacttcct gctcggtacg gcaggcacgt aaacggtcga aattctcttc
     4441 atcttcaaac aggttgacga tctgcatgat gcccacttcc tggtgagtgt tggcatcaac
     4501 cgccgccatg gtgatgagga tatccaccgg atcgttatct tcgtggttga actccagcgg
     4561 ctttttcagc gtcaccagcg caaaaccggt tttcttaacg ccctcttccg ggcgtccgtg
     4621 cggcattgcc aggccggggg cgagcacaaa gtacgggccg tgctgttcaa ccgcatccag
     4681 aatcgcctgg tagtaacgcg gctctaccac atctgccgct accagcagat caacgccgat
     4741 tttcactgcg tcctgccatg tctctgcttc cgcttgcagg cggatagatt tattttcagc
     4801 cagcgaatca cgtaatttca tggcgcgtcc ttacttcacg tcctgcggga aatgctcttt
     4861 gatcacttcc agcagtttcg ggccaaaatc agcaggagag agcatgttgc gcacgccaac
     4921 cacatatttg ttgccggtca cggtgatttc gcccgcaata tgggtagaag cgatgatgat
     4981 atccgcgccg ctcaactcgc ttttgtattc gccaaccgcg cagctgttta ccgtgtggtc
     5041 aatgttcgac tgggttaaaa actggtccac tttcattttc atgatcatgg aactgccttg
     5101 tccgttacca cacacagcca gaatacgaac ggtcataatc aaaactcctt attaagcaga
     5161 ttgttctgcc agttgttttt ctgcatcttc ttctgcgcgc agcgcgcggc tagcgaagaa
     5221 catataagcc agtgcaatta caatgatgac ggccataaag gcgataccga tggagaagaa
     5281 gccctgcatc atcggcggtg ccagaatcga ccagtccgcc atacccatcc aggcactcat
     5341 accggtgagt ttcactgccc agacgcaacc aaagatttcg atcatcccca tcaccagaca
     5401 aattttcagc gccgcacgcc agccgccgaa gtggttagcg aacacgccga tagtggcgtt
     5461 ggagaagaac atcgggataa agccgggaat aatcaggatt gaagagccac aggcgaccag
     5521 gataccaacc gcaatcagtt gcccgatggt gccccacata aaaccccaga ccacggcgtt
     5581 cggcgcgaag ctataaatag ccgcacagtc aatcgccaga accgcacctg ggatcagacg
     5641 ctgggagata ccgttaaatg cttcagagag ttccgccaca aacatgcgca cgccctgagt
     5701 gatgatgaag atcgccaccg caaaggagaa accggtttgc aggatgtaca ccgtccagtt
     5761 cactttgcct gccatcgcct gcacggtgtc gataccaaag gagagcagaa tggcaccaaa
     5821 gaagatagtc atgacaatcg ccgtggagac gatgttgtcg tggaaaatat tcagccagcc
     5881 aggcagtttg aggtcttcaa cgctttcctc ttttttgcca aggaacggtg cgactttata
     5941 agcaatccat gatgcgaact gctgctggtg accaatggag aaaccacagc cgtcggtgac
     6001 ttcctgtgtc ggcttgtaca tcatgttgga ggtaatgccc cagtagagcg aaaccagaat
     6061 cgcggtgcag ataatggtcg tccacatgga gtagccgaag ataaacagcg taacggcaat
     6121 cagccccgcc tgctggaaca tgatgtgacc agtcagcatg atggttcgaa taccggtaat
     6181 gcgacgcagc agcacgtaac agatgttcag cgccagcgct aacaacaccg cgtaacccac
     6241 ccagctatag gcatcgccca tgcgttcaat ggttgccatc atcgacgcgt aagtatcgga
     6301 aattgcgccg ttaatgccgt agacttcgga cattttcgcc accaccggtt tgaaggtgct
     6361 ggtcaggatg ccggaccctg cctgcaacaa catgaaacca attatggttt taatcgtacc
     6421 tttaataata acgctgacac ttttgcgcag taggatgtaa cccagacagg tcacaatacc
     6481 cagcaacaac ggggcattgg tcatgacctg gttaaaaaac acggtaaaga tgttgtagag
     6541 gatctccata acgatctcct gaagaagagg taaccgggta tttcgcacac ccggaatgtt
     6601 gtgctctcac tctaattttc aaaagtaatc acaacaagat tatttatgat taaatgtgac
     6661 gtcgcacgca aataattcca cataaacaga atgggattca cggaatgaaa tatttattca
     6721 tttaaattca tttagttaat tgcattacac attcaattta atctttctac actcaaaatc
     6781 cacaataact ctcctgctaa aaagtcaaaa aatggtcatt gccttcctga aaaaatagtg
     6841 tcaggattaa tcaaaaccaa tcacatattg attcgaattg aactatcacg aaggtaaatg
     6901 gcgatgagta aagtgaaaag tatcacccgt gagtcctgga tcctgagcac tttcccggag
     6961 tggggtagct ggctgaatga agaaattgag caagagcagg tcgcaccagg cactttcgca
     7021 atgtggtggc tcggctgtac cggaatctgg ttgaaatcag aaggcggtgc caacgtttgt
     7081 gttgattttt ggtgcggtac tggcaaacaa agccacggta acccgttaat gaaacagggt
     7141 caccagatgc agcgcatggc tggcgtgaaa aaactgcagc caaacctgcg caccaccccg
     7201 tttgttcttg atccgtttgc gattcgccag atcgacgcgg tactggcgac tcacgatcac
     7261 aacgatcata tcgacgttaa cgtcgctgct gccgtgatgc agaactgtgc tgatgacgta
     7321 ccgttcattg gaccgaaaac ctgcgttgat ttgtggattg gctggggcgt accgaaagag
     7381 cgttgcatcg tcgttaaacc gggcgacgta gtgaaggtaa aagacattga aattcatgca
     7441 cttgatgctt tcgaccgcac ggcactgatc acgctgccag ccgatcaaaa agcggctggc
     7501 gtactgccgg acggcatgga cgatcgcgcg gtaaactacc tgttcaaaac gccgggcggt
     7561 tccctgtatc acagcggcga ttcccactac tccaactatt atgcaaaaca tggtaacgag
     7621 catcagatcg acgtggcttt aggatcgtac ggagagaacc cacgtggtat caccgacaaa
     7681 atgaccagtg ctgatatcct gcgtatgggc gaagcgctga atgcgaaagt agtgatcccg
     7741 ttccaccacg atatctggtc aaacttccag gccgatccgc aagagatccg cgtgctgtgg
     7801 gagatgaaaa aagatcgcct gaagtatggc ttcaagccgt ttatctggca ggtcggcggc
     7861 aaatttacct ggccgctgga taaagacaac ttcgagtacc actatccgcg tggtttcgat
     7921 gattgcttca ctattgaacc agatctgccg ttcaagtcgt tcctgtaatc tgtcgcttgt
     7981 accggatagc gtggagatgc tcgcgctatc cggcatagta tttcctggct atttcaaata
     8041 tcatctaaaa aaatcaaatt ttatcggaat agctcatgac tgaagcacaa agacatcaaa
     8101 tcctcctgga aatgctcgca caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc
     8161 tgggaatttc gcctgccact gcacgacgcg atatcaataa acttgacgaa agcggcaaac
     8221 tgaaaaaagt gcgcaatggc gcagaagcta ttacccaaca gcgcccgcgc tggacgccga
     8281 tgaatctgca tcaagcgcag aatcacgatg aaaaagtacg tattgctaaa gcggcctcgc
     8341 agctggttaa tccgggcgaa agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg
     8401 ggcgggaaat gtgtggcaag ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt
     8461 acctgatcga tcaggagcat gacagcgtga tcattatggg cggacagtac aacaaaagtc
     8521 agtccatcac tttaagcccg caaggcagcg aaaacagtct ctatgccggg cactggatgt
     8581 ttaccagcgg caaaggactg acagcagaag ggttgtataa aaccgatatg ctgacggcaa
     8641 tggcagagca gaagatgctg agcgtggtag ggaaactggt ggtactggtt gatagcagta
     8701 agattggcga acgcgcggga atgcttttta gccgtgccga tcaaatcgat atgctgatca
     8761 ccggcaaaaa tgctaacccg gaaatcctgc aacaactgga agcgcaaggg gtcagcattc
     8821 tgcgtgttta aagatgctgg cggaaaaatg tcacggcagc atctaacgcc tcaggcgtaa
     8881 tgcggtgacg cacgcctggc tgccatgaac aggttagcag tttatcccgt cccgtctcgc
     8941 ttaaggcctg ctgcaaacgt agtgattcgt cggcaggcac gacgtcatcg tcaaggccat
     9001 gccataagag cagcggtctg ttccccaact ctgtcagatg gtttgtcgct tcccactctg
     9061 ccagcggcgc gacaatgtta ttgaattcat tctgctgtgc tgccgtttca ggtatcagcg
     9121 gtggaaacag tgaacgggcg agcgatgtaa aatagcccga tcccatcatg ctggcggtac
     9181 attttaccgt ggggtggcga gcggtaattc ccagtgccgt catcgcgccc atcgacgcgc
     9241 caccgactgc cagacggtca tcacgcagcc agttttcttc ggctattgcc gcacgtaagg
     9301 tagtgaattc ctgcatactt tgtagcaaga tttgccagaa ttgatttaac cgccgcgctg
     9361 cgtcgccact aaaacggcta ccgtgatcgg gcgcatccgg catgatcacc cgcaaaccag
     9421 cctgcgccag cgcaacggca aaatagctat acaccagact ggacgaagta aagccgtgat
     9481 aaaaaatcac acacggtaac ggggtatctt tttgcccgac aggataagca tgaagaacgg
     9541 gaatatctgc cagctcgcgt gattctattt caatcatctg tctctccttt ttgtctggta
     9601 tgacatgaag aaatccttag cacaagtttc tgctggcggt attagttcat aatgttgaga
     9661 tgtgggttac gctttcgtca cgttttcatt cgaaaatcgc gtaccaggta acaattcggg
     9721 aacattcccc caaagttaaa ttaataaggc actacactat ggttagcagg aaacgtaata
     9781 gcattattta ccggtttgcc agtttattat tggtgttgat gttaagtgcc tgtagcgcac
     9841 tgcaaggtac gccacagcca gcaccgccag taacggacca tccgcaagag attcgccgtg
     9901 accagacgca agggttacag cgaataggta gcgtaagcac gatggttcga ggttctccgg
     9961 atgacgcatt agcagaaatc agagcgaaag ctgtcgctgc aaaagctgat tattacgttg
    10021 tcgtcatggt ggacgaaacc atcgtgacag gacagtggta ttcacaagcc attttgtacc
    10081 gtaaataaat cagacaacta acatagtcag ctttacattg ctttgcgtcc ctatgcgttt
    10141 acctgtgcac aataaattac cggctggaag ctcgggcgtt tattaccgcg atggaatgcc
    10201 ctgcaacatg tggggaaacg aaaaatggag ctgacgatga aacaattact tgcctcaccc
    10261 tcgctgcaat tagtgactta tcctgcgagc gccacggcac agtctgcaga attcgccagt
    10321 gctgactgcg tgacgggcct gaatgaaata ggtcagatct cagttagcaa tatttcaggg
    10381 gatccgcagg acgtggaacg tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg
    10441 taccgcatta ttaccatgta cgaagaccag cagcctgata actggcgcgt acaggcgata
    10501 ctgtatgcgt gagtgtcatc tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt
    10561 gtccggccta caattttaaa atatttcatt tagttcatcg aaaccagcgt cgcatcagtc
    10621 gtatacgctt acaaacacac tcctcccgtc gcccgcagca ataaatcatt ctggatctgc
    10681 tctgacaacc gtacgccgcc gcgcgtatct aacatcacct tacaccacgt ctgcgccatt
    10741 ggcggagagg catatttcaa catttgcgca ccgcagccca gcaggaatag ctgatgagta
    10801 atctctcgcc ccaattcttc agctggctta cgcagctgct gctgtaaacg acgaaccgcg
    10861 cgatcaaaat agcgatcctg ccctttcact tccacaaatg cttccgacaa taagtcgtat
    10921 acgcccgctt gcttattgag aacgcgcagc acatccaggc acataatatt gccggaacct
    10981 tcccaaatac tgtttaccgg catctcccgg taaagccgcg gcagctcgct ctcctcgcaa
    11041 taaccaatgc cgcccagcac ctccatcgct tcggccacaa acggaatgcc acgtttgcag
    11101 atcacaaatt tcgccgcagg cgtaaataaa cgcgcccaca gagcttcttt ggcatcggcg
    11161 cgccggtccc acgctcgcgc aagacgaaac agcaacgccg tttgcccttc aagctgaagt
    11221 gccatgcgac ttaagacatg acgcataagg ggctgttgga tcaatggatt accaaaaaca
    11281 tggcgttgat gtgcatgata aatcgccagc gaaaatgcac ggcgcatcat ggcatggcta
    11341 cccagggcgc aatcaaaacg cgtcatccca cccattttca ggatcagacg aattccttcc
    11401 ccttcctgcc ccaacaacca gccaatggca tcctgaaatt ccacttcgca gctggcgtta
    11461 gagcgattac ccagcttatc tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca
    11521 ggcaaaaagc gcggcacaaa aaagcaggac aaaccgcccg cggtctgtgc cagcaccaga
    11581 tgcgcatcgc tttgcggcac cgagaaaaac catttatgcc ccaccagccg ataagaacca
    11641 tcgtccagac gctctgcgcg ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc
    11701 gtcattccca tgccgatcaa caagccacgt ttttgcccac ctggcaataa gtgagaatcg
    11761 tagcgatcgc tcaacagcgg cgtggtccag tcctgaaacg gcgcgggtaa catctgtaac
    11821 aacaatggcg tggcggcaaa ggtcatcgtt atcggacata acgacccagc ctcaacctgc
    11881 gcatgtaaca taaaacgcgc cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc
    11941 caggcaagat tgtgcacccg attggtacat agcgcctgca tcagcaggtg ccaggcgggg
    12001 tgaaaacgca catcgtccag acggcgtcct tgcgcatcgt agcgcaataa ttcaggcgga
    12061 ttcacattcg ccagccgccc cagttcaagg gattcagccg ttcctaactg ctgaccaata
    12121 ctggcgagaa aatcgctatc ccagccagca ccttcacgcg ttaccgcttc gcaaagcgcg
    12181 ccatcagaca ggtataagtt gctgttattt aatggtatag gttgattaaa aacggtgtga
    12241 gtttgccagt gcactgtgtc tccctccatc aatggcagtc accattagta tggtcactgc
    12301 catggattca tgacagaaag agattgctaa aacattctta tccagcaatg taatgcggaa
    12361 cgaaacgtga ggtgtctttg gtgatcagcg tgttatcttc acggatcccc attccgcacg
    12421 cttcatcatc gacaatccag ctaccgatga gtgtgtagct atcgccaaat cgcggcagag
    12481 gttgaaacgc ctggtagatc atcggttcat cggcgtaatc accatcagcg tggtcaacaa
    12541 cgttattctg accgtcgaaa atggtgacgt ttccgccttc gcgcgagtaa atcggtttac
    12601 gcacatagct ttcgccagcg gcaatctgcg gtttttcacc atcgaaccag gatgcaagaa
    12661 ggttaggatg accagggaag aagcgccaaa gcagcggcat tagcccttta ttactcaaga
    12721 tacttttcca taacggctcc acccattgct cacgacgctt gcgcagcagc ggaccgttat
    12781 cgtcacgcat catccactcc agcggataca gcttaaatgc acgctggatg acattatcat
    12841 caagatcggt cagtacgccg ccgacgccca aaccgagctc ttcaatgtag ataaaccgag
    12901 actcctgccc tgcctgctcg gcgcagtcct gcaagtacag cacggtactc ctgtcttcat
    12961 cggtatcctg acagcagcaa aaataaaacg gctcccggct ataaagctcg ctaaagcgtg
    13021 aaatcaggcg ctcctgaatg gcattgtact gatcggcatc gcgcggaata acgccgctgc
    13081 gccgggcatc ttccagccac aaccactgga aataagccga ctcgtacaat gaagttggcg
    13141 tatcggcgtt gtactccagc aacttaaccg gtgcattacc acaccaggca aaatccatac
    13201 ggccatacaa cgaaggatcg cgtgcgcgcc agctttcggc gatcacatcc cagtacaacg
    13261 gcgggatcgc cagttgcgtc aggatctctt catctttaac cgcacgatcc accacctcca
    13321 ggcacatctg atgcagttcc gcagtcggtt tttcgatctg ctcttcaatc tggcgcagag
    13381 taaagcggta agcccgactc tcatcccaat agatttcatt gtcgatgata tgaaagtcaa
    13441 atccgttatc ggcggcaatc cggtccagat cccgccgcac aggaacgttg tgtctcagca
    13501 tgattagcct ccccaatgcc cacgggcgct ggaagaacga ccatagccgc cgcgagaaac
    13561 ggttgacgct tttttggtgg taaagccgcc cgaagagtaa gactcttttt tgccagaacc
    13621 ggagcgccag gagtaatcac cggaagtgtt gcgccagacg gggcgggaag caaatgagga
    13681 tccgccgctg ttatagacga acggctcatc gcgatcttta cgaataaccc ggcttaacaa
    13741 aaatccagaa acgaccggga tccaactctg ttcaacattg tcgtagtaac aattttcata
    13801 cttagactga caattctgct gagtcatatt cttcggaaca tcggcataaa atgccgcttt
    13861 ggcgttgttc cagccatgag cacagatatc tgaattatta ccatcatcaa tacaatcctg
    13921 cacagtcgcg taaaacgttc cgtcgccgtc gttatcgaca tcgccactat cgccacaacc
    13981 tttcaatacg aaaaaagcgg caccgcccat tagcgccaac gttaagtatt tttgtgcgta
    14041 gcgattgcgg cgcggctcaa aaggattatg cggtctacca atgcgactga ttgccccatg
    14101 tccgatattt ttgttatttc tggatttgcg ttttctggcc atgatcgtcc attaccatgt
    14161 catacaagct gcgttaaaaa taccgccagc cagcgcggcg gttcccataa acattccggc
    14221 tgcggtattg tgattaataa ttttttcgct taatgcgggc atataaagcc tgacgccagc
    14281 aaaaacgagt aattgaatca ccagagcaat cccgccccat gcgaaatagt ctggaatact
    14341 caccgcattg attgccgcac tggataaggg gatcacgtac cccaacaatg ttccgctgaa
    14401 cgccagtgcc gccgcggtgt tattgttttt gattaactgc cattcgttgt gcggtgtaat
    14461 tttggagtag ataaacagga aaataatcac catcgccacg ccaataaaaa aataggcact
    14521 aaaagcaaga agtgaatcca gtatatgcat tgtaaacgtc cttgtaatta accaataata
    14581 tgaagtgatg tcagcgggat atcgataccc agcgcgcgcg aaaataacca ctccggctcg
    14641 ccgagatcgt taaaagattc ctcaccattt aacagcaaat attcgtaagt atctccggtc
    14701 acctgccgtt ggtagcccat gctaaaatta tgcacttccc atttggcatg gttttgattt
    14761 tctacttttt ccagcatgta gacgggttcg atatttcccg gttcttcgct gttaaaaaat
    14821 cgctgccagc gtttttcctg ccagtttaag gtcattgccc ccatcgcttt ggcgttgatc
    14881 gcctcgcgcc agtgactctc tttactgata ccgtagctct cttcatagac aaagagcttg
    14941 atatcatcaa tgtcatcaat atcttcgccg ccagtggtat tgatttgcag gaactcatcg
    15001 cccgaagtgt agtaacggaa aatctggcta cccccgccca gatcgatgtg gctgacagcg
    15061 gctaccgtaa attcctcacc cggcagcgca atcagcaatt catcttccag caaacgaaac
    15121 gataatgtgt cgagcgtaaa gccactattg agatgcagac ccagcggacc gcgagcgata
    15181 gcaggcttat tatccttgcc aaacagacgc tggaaaaaac cagacatatt ttatccttaa
    15241 aatagttacc agccttgcag tgaggctggc aaaaaattac tcgccctgtt ggcgttgcag
    15301 tcttgctaat acatcctggg cgctactttt attgctaccg ccaattccag cttccgccag
    15361 cttttcatcg agatcgcgac cgtccgcgac tttttccaac tgtgcggcag catccaggcg
    15421 agcctgacgt tcggcctgac gcgtttgcag gcgttttaag gattctgccg ctgtcgaaac
    15481 gctggaagat gcgccaacgg tagaggtcgt tacagcctgt tgtgcacgct gcatggcttc
    15541 agtggcttta acgacttcca tttgctgctc aaactgagcg atacgctgcg ctgtcgctgt
    15601 aactgctttt tccacgccat cacgagagac ttccagattc gacaacactt gctcttcagc
    15661 ggtaatgaga ttctcaaggc gcgcgatttc ttcagcaact tcgttaatca acgacggatt
    15721 aacgttcttg cttaacgctt ccagcgcacg cgcttccaga ctggctttgc gctcacgtaa
    15781 atctttcagc ttatcgtgac ttaatttcac ccgtgccagc agatcaacgc gagattttcc
    15841 ggctttatcg agttcagctt tagcgtcacg aatatgctgt tccagcatgc gcacgccctg
    15901 ggtttcttca atggactctt ccgcctggga gataaacgat ttccccagcg taaataaact
    15961 ttttaaaatt cccataaata cccgtccttg taattagtgt gaatattctt ctgtaatttc
    16021 agccagatcc aatgcgttat ctaccagcga ggttatctca agcaggatat cttcaagaga
    16081 agatttaagc gataacgcac cgaaaacaat gtaatactct tcctgttgca cgctggagat
    16141 ccctaccgat gacaacggca tcatcttctg atttcttaat aagaaggtat taaattcatc
    16201 tggattgctg atactactta ccggacaaat aaaggtttcg atgatcattt ggcgggaagt
    16261 aaaaagaata ttgatttgca aatcgccata atcattcagt ttaataatta atgcactttc
    16321 gttatttgtt acatcaatat tttgttcagg tacagtttgc agcgccgtcg ccagcgccaa
    16381 cggattccat gtcatatatt attccatata gattaagttt aaatattaat aaaatgaata
    16441 tttgcagtac gtaattatct taccagctat agacaaaaaa aaaccatcca aatctggatg
    16501 gcttttcata attcagagga actagctgcg ctgacgaacc gcttcaaata agcaaattcc
    16561 ggttgcaacc gaaacgttca gggaagaaac acttcctgcc attgggatgc tgatcaactc
    16621 atcgcaatgt tcacgggtca ggcgacgcat accttcacct tccgcgccca tcaccagcgc
    16681 caggcgtccg gtcattttgc tctgatagag tgtatgatcc gcctcgcctg ccgtaccgac
    16741 gatccagata ttctcttcct gcaacatacg catggtgcgc gccagattag tcacccgaat
    16801 cagcggaacg ctttctgccg caccacaggc cacttttttc gccgtggcgt tgagctgtgc
    16861 ggagcgatct ttcggcacaa tcaccgcatg aacaccagcg gcgtccgcgc tacgcaggca
    16921 cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc agcaggaacg gttgatcgag
    16981 cgaagcgatc agatccggca gatcgttttc ctgatactgc cgtcctggct tcacacgagc
    17041 gataatgccc tgatgcacgg caccgtcgct tttctcgtcg agatattggc ggtttgccaa
    17101 ctggataacc acgccctgag actcaagggc gtgaatcagc ggtaacagac gtttatcttc
    17161 acggcctttt aaaataaaga cttcctgaaa acgttcaggg gcgcgctcca gcagggcctg
    17221 cactgcatgg atgccgtaaa tcatttcgct cattaatgta ctcgttgttt acgttttgcc
    17281 gggagcgcca cgcttgcccg gcctacggat aattcaccct cttctgtttg aagagggtaa
    17341 tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt
    17401 ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgctg ctttcggctt
    17461 cggttttttc tcgccacgga aggcgctgtc tggctcaaag ttgacctttt tgccgacctg
    17521 acgccgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt
    17581 accgacgttg cgcggtgcgc gttcgctgga gatcaggcta aagtcgattt tgcgctcgtc
    17641 catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctgaccgct
    17701 ggattcaccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga
    17761 agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc
    17821 agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca
    17881 cttcagccag tcagacacat cgcgcgttgc ttcatcggca cgacgttccg ccatcgaaca
    17941 gtgctgaccc agttgcagca tctcttccat cgaataatgg tagccgccgg tttcagtggt
    18001 gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt gcaacgtcag
    18061 gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa
    18121 gtgaccacgg ttttccggat cgtaaatcgc ctgtttcatc gagcgcaaaa gcatggtttg
    18181 cagcatttct gcatcaggac gatcggcaac cgattccagt aactctgcat agtcgcgcgg
    18241 ttccggcttg ttaccgcccg gcaattccag ccccagctcc gccagcactg aacggaaaga
    18301 ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttggcttt
    18361 ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaacttgtg
    18421 ggcgtcgtta cgctgagtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa
    18481 cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac
    18541 tttatagagg ttatgtaact cttcgagatg cttaaccagc ggcgcatact gctcgcgcag
    18601 atcctgatcg ccttgcagta tatgccagac tttggtgtag gtcagacgcg cgtgagaact
    18661 catcaccgct tcgtagaatt tgtagccagt caggcggcct ttcgacgaaa ccgtcatctc
    18721 gcacaccata cacaggcggt ctacctgcgg gttgagcgag cacaggccgt tagagagcac
    18781 ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtaccac ggttacgcgc
    18841 ttctctgtcc agcggtgttg gcggacggac atagtaactg acgtcggcaa ttgcgaccca
    18901 caaacgccag ccaccgccgc gttttttctc acagtaaact gcatcgtcaa agtcacgggc
    18961 gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaga tcgacacggc ccgcttttgc
    19021 ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct gcggccagat
    19081 gtacggaatt tcatgggtac gcagagcaat atcaaccgcc atgccggtgc ccatattatc
    19141 gcccagcact tcgacgattt tacccaccgc tttggtacgg cgagtcgggc gttgagtcag
    19201 ttcaaccacc accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat
    19261 atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtgaagta
    19321 gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg
    19381 acctttacgg tcagcgccca gcggctgagc cagcacctga tcgccatgaa tgcaggtttt
    19441 catctgctcg ctggagagat acaaatcatc tttacgccct tcaactcgca gaaagccgta
    19501 gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata
    19561 gcactgacgg cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcgtcgacg
    19621 caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct
    19681 ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt
    19741 cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt
    19801 gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc
    19861 tcttcaacca aatcggcaag cgtgtagtta tccagttccg tcagaaaact ttgcacggcc
    19921 ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa
    19981 ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc
    20041 ggtttaccca agcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga
    20101 ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact
    20161 tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat
    20221 ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc ggggaaaaat
    20281 acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg
    20341 aaggaaaaaa acgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc
    20401 aatgtatcac actgttagcc cggcaggcaa aatatctgcc atgcgtacca gaattacgcg
    20461 tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg
    20521 atcggcacac cggtcagctc ttcaatgcgc ttgatgtagt tcagtgccgc ctgcggcagg
    20581 ccactacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc
    20641 ggctctagac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc acgaccatcc
    20701 ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta
    20761 gtcaggcaga agccagacag ggagttcaac tgtaccgcac gacgaacggc aacggtgtcc
    20821 agccagccgg tacgacgacg acgacccgta gttgcgccga attcgttacc ctgcttgcag
    20881 aggaactcgc cagtttcatc aaacagttcg gtcgggaacg gacctgcccc cacacgagtg
    20941 gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg
    21001 gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggatatgt accgtggtcg
    21061 atatccagca gcgtaccctg cgcaccttca aacatgacga aatcgccacg ctgacgcgcc
    21121 tggtcgagca ggtcagaaac gtcaacaacc atagaagtca ggatgtcggc aacagccatc
    21181 gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg
    21241 aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg
    21301 tcgccaacac gcagaccgcg acgtgctact ttatcttcat aagcaggccc gataccacga
    21361 ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagcgcaacg
    21421 tgataatcaa ggatcagcgg acatgcttcg gacagcagca gacgctcacg aacggggatg
    21481 ccacggtctt ccagttcttt catctctttc atcagcgcag ccggagacag cacaacaccg
    21541 ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg
    21601 gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca
    21661 acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat
    21721 tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat
    21781 ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat
    21841 cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc
    21901 aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga
    21961 gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac
    22021 cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc
    22081 accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg
    22141 cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctgtccgggc tcatgaccat
    22201 cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta
    22261 gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttctg catcgccttc
    22321 accacgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc
    22381 ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgtgctac
    22441 cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt
    22501 gatctgctta attcgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt
    22561 gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc
    22621 gttatctgct gcaggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc
    22681 gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag
    22741 gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg
    22801 cgaaatgtcg ccaccacccg ttgccagata gtaacggctg aaatcgctga tgcgccattt
    22861 gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt
    22921 gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa
    22981 atgcagaccc ggctcataaa ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa
    23041 acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac
    23101 cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg
    23161 tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac
    23221 gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg
    23281 ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaat
    23341 ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttgcccaac
    23401 actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat
    23461 tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc
    23521 ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact
    23581 tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc
    23641 gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc
    23701 agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg
    23761 ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataacc
    23821 ccgcgtaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac
    23881 agatattttt ccggatcggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc
    23941 tcgtccgacg tcagcatcac accagaagct gccagttcac gcacggcttc cacgttcacc
    24001 ggtttgactt cgtcaataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg
    24061 aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg
    24121 gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacgcgacc gccaagctgc
    24181 gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc gccacccaga
    24241 ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg
    24301 cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg
    24361 tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt
    24421 tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct
    24481 acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc
    24541 aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc
    24601 tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggcg gcagacgcaa
    24661 tgtatgctgc gccacctcgc cggaaagacg ctccgtcaaa gcctgaaaaa gctgtggtat
    24721 ccccgctccg gtctgcgcag aaagccagac acggatcggt ttgttctctt catctctgtc
    24781 aatacgcggt tcgaaatcgt ccagcatatc gatcttgttc atcaccagca gggttgggat
    24841 ctcgtgagca tcaatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg
    24901 cacatccgcc gcgtcaatga cgtgcagcag caatgtggct tgccgcgtct cttgtaacgt
    24961 ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc
    25021 caacacggtt tcaccgacat ccgcaacgtc aatacgccgc aacgtcgggt cgagggtggc
    25081 aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt
    25141 accggcgttg gtatatccca ccagcgaaac agtaggaaca tcggctttga tacgcgattg
    25201 ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg
    25261 attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaaccgat
    25321 cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg
    25381 caactgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat
    25441 atctaaaata aggccggtgc ggtcgatgac acgacactcg cacaaacgct ccaggttacg
    25501 ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg tcgcttttac
    25561 ggcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt
    25621 acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa
    25681 ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg
    25741 ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac
    25801 gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
    25861 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tgatagttac tgctggtacc
    25921 gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag tagaaatcgc
    25981 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
    26041 ctcgatttgt ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    26101 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    26161 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    26221 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
    26281 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    26341 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    26401 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    26461 aatccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    26521 ccaacatctg atgaaaacgc tgctcaattc gttgatggag cagttcacgg ctcgccgggg
    26581 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    26641 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    26701 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    26761 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg tacttccggg tccgccgacg
    26821 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaata
    26881 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    26941 cagcagccga gtaagcctgc gacggatcgc gaatattcag caatcggtgc ggcgcggcga
    27001 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    27061 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgctgttt
    27121 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    27181 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    27241 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    27301 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    27361 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    27421 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgcgcatcg
    27481 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    27541 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gttgggcgca aactggcact
    27601 tcacccggcg tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt
    27661 gaaatgttgc catcgcgctc aagcaacgca cagtcggaat ggacgatagt cagtacccga
    27721 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttctggcgc ttttggtttt
    27781 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtaa
    27841 cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgaggaggg cgcaggagtg
    27901 taacgcggag ctactggctc acgaactgcc ggttcagcaa aatgattgcg ccccgccgcc
    27961 acgcgatttt ccggaatggc acgcggtgca ggttgtggtt catcgtccag cggtagcggc
    28021 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaagtc atgcaccaga
    28081 cgcgactgat ggaaacgcac ttcgtgtttg gcagggtgca cgttgacgtc cacctgatgc
    28141 gggtcgatct ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    28201 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    28261 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgc
    28321 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg
    28381 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactggcgt
    28441 acaattttgc cgttatgcga caggttgata gtgacgtcga aacgcgccag cgcaatgcgg
    28501 cggatgattt catcgatatg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    28561 gccggggtgt tgtagaacag gtccagcacc tccagcgtcg ttcccaccgg atgcgccgcc
    28621 ggtttaaccg tcacgtccat attgcgccct tcggcataag cctgccaggc ttcctgctgt
    28681 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagtgcctca
    28741 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    28801 gcatgacgcg ccagcgccag tgccagctca tcttttttga taccgcagcc gttatcacga
    28861 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    28921 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    28981 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    29041 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    29101 ggcgcagatt gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct
    29161 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttactgata
    29221 aaaccggttt cgaccagtac tgacggaata tccggcgaac gcagaacgcc gagactcgcg
    29281 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    29341 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    29401 aaataggggt cggcctgact gttcgccagc acatcacccg ccccgcccag cagctccgac
    29461 tgcttctcat gctgttccag ccagctggcc atttcgctgt tggcgcgacg gtttgagagc
    29521 acccatacag aggcaccggt ggcactacgg tttggtgcgg catcggcatg aatcgacaca
    29581 aggaaattgg cgttttgctt acgtgccaca tcactgcgcc ccatcaccga gataaagtaa
    29641 tccccgtcac gggttaaaac gcccttaaac atcggatcgt tattgagcaa ggtacgtaat
    29701 ttacgcgcaa tggcgatggt gacatttttc tcccgtgtac caccagggcc gatagcgcca
    29761 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc tgtgttagcc
    29821 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    29881 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    29941 gtttcaacgc gtttcgcaac cattggcgga ggcgatggcg gcgcatcggc gttaatcgta
    30001 aagacaacag tgtaattgct gccattctgc cgcttcaccg cttcggtttt accgttttcg
    30061 gtgagatcga ccaccagccg tagcgtttgt gcatctttag gcgttccgga gcgaatcgct
    30121 ttcaccaggt tattaccgct aaacaacaac ggcagcccct gaattacccc cgtttgtttg
    30181 atatcgagcg ccacggtgcg tttgctttga tggctaaacg cataatcagg atcgccaata
    30241 aaactcaacg ttatccgcgc ctgttgatta ccgttagaaa cctgaatatc agagagcgtc
    30301 gcggcccccg cctgcgcgca cagcagcagc aacgtcgcta ctaaccaatt tctgatgcga
    30361 tacatcatcc cgccaccttt caaaggttaa ccggctaaac gcgccagcaa caattcaccc
    30421 gcagaggaaa ccgcactcac gcgcgcctca cggccttgtg cctgataatc aatgtgtatt
    30481 tcgacatccg ggtcaggaag aacacctgta ccttgttgtg gccactccac caggcagatg
    30541 gcatcgttgg caaaataatc gcggatcccc ataaactcca gctcctcggg atcggcaagg
    30601 cggtacaaat caaagtgata aaccattaaa ttgtcgagcg tatagggttc gaccagcgta
    30661 taagtggggc ttttgacatt accctgatga cccagagcct gtaaaaagcc ccggctaaag
    30721 gtggttttac ctgcgcctaa atcgccatac agatagatta cggttgcgcc atcgcaggct
    30781 ttcgctaccc gctcgcccag gtctaatgtt gcctgctcat cagggagcgg aattactcga
    30841 ttcatcatgg tttttatcag tcacttccgg gttaacaata cgctgtagcg tggaaaagag
    30901 atcggttgcc agcatcccgc gcgttccaaa acgcgccgcc agtacgtcag ctgccgcacc
    30961 gtgcgcgaca cagcccgcac aggctgcatc ataagggctc attttttgcc caagcaatgc
    31021 gccaataata ccagagagca catcgcccat gccgccgctc gccatgcctg catttccggc
    31081 atcaataatg cctaaagcgt caggatgggc ggcgaccacg gttccggcac ctttcagcac
    31141 cgctacgccg ccataacgtt gtaccagacg ttgggcgcaa tgtaagcggt cactttcaat
    31201 ttcagcgacg gaacatccta acaaccgtgc ggcctcacca ggatgcggcg tgatcacgcg
    31261 attgtgacgc ttatcgggat tgattgccag caggttcaat gcatcggcat cccacaacat
    31321 cggtttgcga aaattctcaa ctttttgcag ggcttttttc ccccactctt gctggcccag
    31381 accgggacca atcaccacca catcggccca ttccaggctt tcggtaagag agcccatagt
    31441 cagttcatgc accatcaatt ccggtcgtgc agtcagcagc ggcgcaatgt tctcactgcg
    31501 ggtcagtact cggactaaac cagcaccagc acgcagcgcc gcttcccccg tcatacgaat
    31561 agcccccgcc gtgccgtgat cgccaccaat aatcaccagc cgcccgtgat cgcctttatg
    31621 cgaagtcggg cgacgcggta tcagccactg agaaagttgt tctgccgaaa accgctgaat
    31681 tttcgtctcc tgacctgcca gccatctatc cagccgcagt gagtcaaaat gcagttgtcc
    31741 ggtaacatcc cgcgcttttc cagtgagcaa acctggtttc agcgcaataa aagtgatggt
    31801 gtgatcggcg ttgattaccg cgcctggcgt agcgccggtt tcagccagaa ggccggaagg
    31861 gatatcaacc gccacaatcg gcgcaggatg ggaattagcg tggtcgatta actggctaat
    31921 ggattcgcgg ggcgcttgct gcaaaccggt gccgagcagc gcatcgacaa tcagatctac
    31981 cgattcgggc cagacaatat tcgaagcatg gatctcgcct cccgcgttta accatgcttc
    32041 gcgtgccagc gcggcctctt ccggcaacgg tttgtcactt tcctgggcca gcagcgtgac
    32101 atcaataccg atcgctttgg ccagccgcgc gaccacgtag ccatcgccgc cgttattacc
    32161 atgaccgcac agcaccagcc agtggcgggc gtcaggataa gccgaacgac acacctggaa
    32221 tgcggcttcg ccagcgcgaa gcatcagctc atagagtgtg agccccagcg catctgccgc
    32281 ctcgcgttct ccgcggcgga tatcgtcggc gtaccagacg gtgtgtggta tacttacggg
    32341 gtttttcttc attgtatggt ccgtcatgtc agagcccctc gatctcaatc agttagcgca
    32401 aaaaattaaa cagtgggggc tggaactggg ctttcagcag gtaggtatta ccgataccga
    32461 tctcagcgag tccgagccca aactgcaagc atggctggac aaacaatacc acggcgaaat
    32521 ggactggatg gcgcgtcacg gtatgctgcg tgctcgtcct cacgaattat tgcccggtac
    32581 gctgcgcgtg atcagcgtgc ggatgaatta ccttcctgct aacgccgcat ttgccagcac
    32641 gctgaaaaat cccaaactcg gctatgttag ccgttatgcg ctgggccgtg actatcacaa
    32701 gcttctgcgc aaccgactca aaaagctggg cgagatgatt cagcaacatt gtgtttcgct
    32761 gaattttaga ccgtttgtcg attctgcgcc tattctcgag cgcccgttag ctgaaaaagc
    32821 aggtcttggc tggacaggta agcactcact tatcctcaat cgcgaggccg gttcgttctt
    32881 ctttttaggc gaattgctgg tcgatattcc gctgcccgtg gatcaaccag tcgaggaagg
    32941 atgcggtaaa tgcgtggcct gtatgacgat ttgcccgacc ggtgccatcg tcgagccata
    33001 taccgtcgat gctcgccgct gtatctctta tctcaccatc gaactggaag gggagatccc
    33061 ggaagagttg cgaccgttaa tgggaaaccg tatttacggt tgcgatgact gccagcttat
    33121 ctgcccgtgg aatcgctatt cgcaactcac tacagaagac gatttcagcc cgcgtaagcc
    33181 gctacacgca ccggaactca ttgagttatt cgcctggagt gaagagaagt ttttaaaagt
    33241 cacggaaggt tcggcgattc gccgtatagg tcacctgcgt tggctgcgta atatcgccgt
    33301 agcattaggc aatgccccct gggatgaaac gattttgaca gcgctggaaa gtcgtaaagg
    33361 tgagcaccca cttcttgatg agcacatagc gtgggcgatt gcgcagcaaa tcgagagacg
    33421 aaatgcgtgc gtggtcgaag tgcaattacc gaaaaaacag cgtctggttc gggtgattga
    33481 aaaagggtta ccgcgtgacg cctgattcat ccacagcttg tgaataaaaa taaaaacgca
    33541 ttgcaattca agccggaaaa aatcgtcaag tgatcgatac gacaaaacga aatcttaatt
    33601 tttaacttat ctatcaatag gttaaaaaaa tactattcac cgtgcgaagt ttctttgaaa
    33661 ttaagattac ggcctgaggc tgtggataac tctgtttaca agagtttgtc agagacaaca
    33721 aaaaccagca ataacgcatc gcgtcgctgt ggatatttta ttgagagaag aatttggagc
    33781 gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag
    33841 ctattcccgc ttgggtggtc tgtgctttac agcactttca aattttggag cgggaaacga
    33901 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
    33961 cttgggtggt ctgtgctttg cagcactttt caaattttgg agcgggaaac gagactcgaa
    34021 ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcgtactac
    34081 ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta cgggaggcgc attatacgag
    34141 aaatctttcc ctctgcaacc cccctaaaaa attttttttg agtttttatc tcaagtgctg
    34201 aaattatcag caaggcgttc aatttagcgt caaaatcacg ccgccgcaag tggattccgg
    34261 cgcttacgtg accaggaaaa attttacagc ttgataaaat gctcgcggta gtaagccaac
    34321 tccgccaccg attcacggat atcatccatc gcctgatgcg tcccctgctt ggtaaagccg
    34381 tcaagaattt ccggcttcca gcggcgcgcc agctctttca gggtgctaac atcgagatag
    34441 cggtagtgga agtaggcttc cagctccggc atgtatttaa acaggaagcg acggtcctga
    34501 ccgatgctgt taccacaaat cggcgatttt cccgcaggca cccactgttt taagaattcg
    34561 agcgttgcca gttcagcttc acgatcgccc atcgtgctcg ctttcacacg ctctaccagc
    34621 ccgctggcgg tatgggtgcg cacgttccag tcatccatca gcgccagctg ttcatcggac
    34681 tgatgtactg caatggtcgg cccttctgcc agaatattca ggttggcatc ggtcaccagc
    34741 gtggcaatct caataatgcg atcgcgctcg ggatccagac cggtcatctc aagatcgatc
    34801 caaatcaggt tgttttcatt ggcactcatg ctattttcca cccattcaca gtgactatat
    34861 tgatccacaa ttgcgtgtat catagatgtt ttgcccatca ggggcgacca ggagtctgta
    34921 cgattgagta aaaataaact ctccaaaggc cagcagcgcc gcgtgaacgc caatcaccag
    34981 cgtcgtctta aaacgtctaa ggagaagccc gactacgacg acaatctgtt tggcgagcct
    35041 gatgaaggta tcgtcatcag ccgctttggt atgcacgctg atgtggaatc cgccgatggc
    35101 gacgttcacc gctgcaatat tcgccgtacc atccgttcgt tggtgaccgg cgaccgcgta
    35161 gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg tcaaagggat cgtggaagcg
    35221 gtgcatgagc gcacctcagt gttgacgcgc ccggatttct acgacggtgt gaaacctatt
    35281 gctgccaaca tcgaccagat tgtcattgtc tccgccattt tgccggagct gtcgctcaat
    35341 attatcgacc gttacctggt ggcctgcgaa accttgcaga ttgagccgat tattgtgctc
    35401 aacaagatag acctgctgga cgacgaaggc atggagttcg tcaacgagca gatggatatc
    35461 taccgcaata tcggttatcg cgtattgatg gtttccagtc atactcagga tgggctgaaa
    35521 ccgctggaag aggcgttgac cgggcgcatc agcatttttg ccgggcagtc tggcgttggc
    35581 aaatccagcc tgctgaacgc gttgctgggt ctgcaaaaag agatcctgac caacgatgtc
    35641 tctgacaact cggggcttgg tcagcacacc acgaccgccg cacgactgta tcacttcccg
    35701 cacggcggtg atgtgattga ttccccaggt gtgcgtgagt ttggtctctg gcacctggag
    35761 ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact atttaggtct gtgtaaatat
    35821 cgcgattgca aacacgatac cgatccgggc tgcgctatcc gggaagcggt tgaggaaggg
    35881 aaaatcgcgg aaacccgttt cgaaaactat caccgtattc tggaaagcat ggcgcaggta
    35941 aaaacgcgta aaaacttttc tgatacggat gactgacaat taagctaacc gtcgttagaa
    36001 tcgtcccctt tttttcagga tccggcatgt aggccggatc aggaacgaca aaacaatggc
    36061 ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac agtacattct gccgaaacta
    36121 tggcttactc gcctggcggg ttggggcgca agcaagcggg caggatggct gacaaaactg
    36181 gttatcgatc tgttcgttaa atactacaag gtcgacatga aagaggcgca aaagccggac
    36241 accgccagct accgcacctt taacgaattc tttgtccgtc cgctgcgtga tgaagtacgc
    36301 ccaatcgata ccgatccgaa tgtcctggtt atgcctgccg atggcgttat cagccagttg
    36361 ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc acaactacag cctcgaagcc
    36421 ctgctggcag gcaactatct gatggcggat ctcttccgta acggtacgtt tgtgaccact
    36481 tacctctccc cacgtgacta ccaccgcgta cacatgccgt gcaacggtat tctgcgtgaa
    36541 atgatctacg tgccgggcga tctcttctcc gttaaccatc tcacggctca gaacgtgccg
    36601 aatctgtttg cccgtaacga acgcgtgatc tgcctgttcg ataccgaatt tggcccgatg
    36661 gcgcagattc tggtcggtgc gacgattgta ggcagcattg agacggtatg ggcgggcacc
    36721 attacgccgc cgcgcgaagg catcatcaag cgctggacct ggcctgccgg ggaaaacgac
    36781 ggttctgtgg cactgctgaa aggccaggaa atgggtcgct ttaaactagg ctctaccgtt
    36841 atcaacctgt ttgcaccagg taaagtggac ctggttgagc aactggagag cctgtctgtc
    36901 acgaaaattg gccagccgct ggcagtatct accgaaacct ttgttacgcc agatgctgaa
    36961 ccagccccgc ttcctgctga agagatcgaa gcagaacacg acgccagccc attggttgac
    37021 gataaaaaag accaggtcta atccatcaaa ggaaacgctg acgtgcgcct gattatcact
    37081 tttctgatgg cctggtgcct cagttggggg gcgtacgccg cgacggcccc cgatagcaaa
    37141 caaatcaccc aggaactgga gcaggcaaaa gcggcgaaac ctgcgcagcc ggaagtcgta
    37201 gaggcgctcc agtctgcctt aaatgcgctt gaggaacgaa aaggttccct tgagcgcatc
    37261 aagcaatatc aagaagtcat tgataattac ccgaaacttt ccgccactct gcgcgcgcag
    37321 ttaaacaaca tgcgtgacga gccacgcagc gtgtcgccgg gaatgtctaa cgatgcgctg
    37381 aatcaggaaa ttctccagat cagcagtcag ttgctggata aaagccgtca ggcccagcaa
    37441 gagcaggagc gcgcccgcga gattgccgat tccctgaatc aactgccgca acagcaaacc
    37501 gatgcccgcc gtcagttgaa tgaaatcgag cgccgcctgg ggacgcttac cggcaatacc
    37561 ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact ccgcacgtct gaaagcgctc
    37621 gttgatgaac tggagctggc gcagctatct gccaataacc gccaggaatt agcgcgcttg
    37681 cgctcagagc tggccgaaaa agagagccag cagctggatg catatttgca agcactacgc
    37741 aaccagttga atagtcaacg ccaactggaa gcggaacggg cgctggaaag taccgagcaa
    37801 ctggcggaaa gtagcgccga tttgccaaaa gatatcgtcg cccagtttaa aattaaccgc
    37861 gaactatcgg cggcactgaa tcaacaggcg cagcggatgg atctcgttgc ctcgcaacag
    37921 cgtcaggctg ccagccagac gttacaggtc cggcaggcgt tgaatacgct gcgtgaacag
    37981 tcgcaatggc tgggatcatc taacctgctc ggcgaagcgt tgcgggcgca ggtggcacgg
    38041 ctaccggaaa tgccaaaacc acaacagctt gataccgaaa tggcgcagtt acgtgtgcaa
    38101 cggttacgtt atgaggatct gcttaataaa cagccgctgc tacggcaaat ccatcaggcc
    38161 gacggtcagc cgctgaccgc tgagcaaaac cgtattctgg aagcacagct acgcactcag
    38221 cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc tactgctgga gctgaccaag
    38281 ctgaaagtct ccaacgggca actggaggat gcactgaaag aggtgaacga agcgacgcac
    38341 cgctatctgt tctggacctc tgacgtgcgc ccgatgacca tcgcctggcc gctggaaatc
    38401 gcccaggatc tgcgtcgtct catttcgctg gacaccttca gtcagttggg caaagccagt
    38461 gtgatgatgc tgaccagtaa agagacgatt ttgccgctgt ttggcgcgtt gattctggtc
    38521 ggttgcagta tttactcgcg ccgctatttc acccgttttc ttgaacgttc ggcggcgaaa
    38581 gtcggcaaag tgactcagga tcacttctgg ctgacgttac gcactctttt ctggtcgatt
    38641 ctcgtcgcgt caccgctgcc tgtgctgtgg atgacgctgg gttacggctt gcgcgaggcg
    38701 tggccttatc cgctggcggt cgcgattggt gatggcgtaa cagccaccgt gccgctgctg
    38761 tgggtagtga tgatttgcgc cacctttgct cgcccgaacg gcttgtttat cgctcatttt
    38821 ggctggccgc gcgaacgtgt ttcccgtggg atgcgttact acctgatgag catcgggctt
    38881 attgtgccgc tgattatggc gctgatgatg ttcgataacc tcgacgaccg cgaattttcc
    38941 ggttcgctgg gacggctttg ctttatcctc atttgcggtg cgctggcggt ggtcaccctc
    39001 agcctgaaaa aggccgggat cccgctgtat ctcaacaaag aaggcagcgg cgacaacatt
    39061 accaaccata tgctgtggaa catgatgatt ggcgcacctc tggttgccat tctggcgtcg
    39121 gcggtgggtt atctggcaac ggcgcaggcg ctgttagcga ggcttgaaac ctcggttgcc
    39181 atctggttcc tgctactggt ggtttatcac gttatccgcc gctggatgct gatccagcgc
    39241 cgcaggctgg cgtttgaccg ggcaaagcat cgccgggcag agatgttagc gcagcgtgcg
    39301 cgtggcgaag aggaagcgca tcatcacagt agcccggaag gagcaattga agtcgatgaa
    39361 agcgaagtcg atctcgatgc catcagtgcg caatccttgc ggctggtgcg ctcaattttg
    39421 atgttgatcg ccctgctttc ggtcattgtg ctgtggtcag aaatccattc cgctttcggc
    39481 ttcctcgaaa atatttcgct gtgggatgtc acttccacgg tacagggcgt agaaagcctg
    39541 gagccgatta ccctcggtgc ggtgctgatt gccattctgg tgtttatcat caccacgcag
    39601 ctggtgcgca acctgcccgc gctgctggaa ctggcgattc tgcagcacct ggatttaacg
    39661 ccgggtacgg gctacgccat caccaccatc accaaatatc tgctgatgct aattggcggg
    39721 ctggtcggct tctcgatgat tggtattgag tggtcgaaat tgcagtggct ggtcgccgcg
    39781 ctcggtgttg gtctcggttt tggtttgcag gaaattttcg ccaactttat ctctggcctg
    39841 attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg taacgatccg cgatcttacc
    39901 ggtagtgtga cgaaaattaa cacccgcgcc accaccatca gcgactggga ccgcaaagaa
    39961 attatcgtgc cgaacaaggc gtttattacc gagcagttta tcaactggtc gctctctgac
    40021 tcggtcacgc gcgtggtgtt gacgattccg gcccctgcca atgccaacag tgaagaagtg
    40081 acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg tgatcgacaa cccggcgccg
    40141 gaagtcttcc tggtggatct gcagcagggg attcagattt tcgagctgcg tatttacgcc
    40201 gctgagatgg gtcaccgtat gccgctacgc catgagattc accagctgat tctggctggc
    40261 ttccacgccc acggtatcga tatgccattc ccgcccttcc agatgcgtct ggaaagcctg
    40321 aatggtaaac aaacggggag aacattaact tctgcgggca aaggtcgtca ggcgggaagt
    40381 ttgtaaggtc tttgtggccg gatgaaccca tccggctcac aagcaaaact gatagcgcag
    40441 aaaaaactgc cacaaccagt agccgaaaat agccagcgcg gtggcgtaca taaaccagcc
    40501 gcgttttttt cgccacaaag cgaaccagag aaaagggaaa aagagcggta acgtcattat
    40561 cacaccgaca tcgcgagtat cgtcatcttc gagatacatc aaaacatcac agacgttgtt
    40621 aatcccgcgt ccatcaatca tccactcttt gtccagcgcg gaggtaatgg ttatccagag
    40681 gaacgccaca atgaaccaga tgcagcacaa gacagacatg cgcgcactca tgaggtaacc
    40741 catagtttaa tttcgctata gcgcatacga aatctcacta attccctgtc attacgccct
    40801 atcgtcaggc cgacgatagg gcatttgatt actcagccac ccactgcgga ttttttacca
    40861 ctctgctgca atagttctgg taaatcgcca tcgctaacag cgagaagaag acagggccgc
    40921 caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggttga atgatggtga
    40981 agacgttcgc aaatgtcacc accagtacga caaccactgt cgcaatcatt gccgacatac
    41041 gcgttttgaa aatcacaaac ggtctgtcga gatcctgacg tgctttaaag aacgggaaag
    41101 ccagcgcgag gaacaggtaa ggaagcgtca tagacacgtt cgccatcagc gtcagcttgt
    41161 taaagaacgc cgatgcggta ccgccaccaa acgaaaccag caggatgaag acagtaacca
    41221 acccacactg catccacatg gcgatagacg gcatccccat cgcattcagg cgcgtcatcg
    41281 gttccggcca caatgctttc ggcgtcccct ggatgatggc tttcagcggt gaatagcaaa
    41341 gcgtaaagaa cgcaccggta taagcgagga acatcgacag tccggtaata cgcgcaaacc
    41401 atacgcccag cgacaatgac gcttcaggtg acaaatgcag tgcgttaccc agcgtcaccc
    41461 cgaggctctt catcagcaca taggtaatat tgccgaggtt aacggaacca ttacttaata
    41521 cctgctgcca gtttgtgctg acgccccata aaaatattgc cagcgaataa ccgattgaaa
    41581 taacaatagc ggcaaaaaca atacctttgg caaagttctt ttctggattt tccgttttat
    41641 caaccagacc accaaccgct tcaattccgc cataggcaaa aatggcaaat accacaaacg
    41701 atagcattgc cagaccggac tgataacctg ggttcggtga tgcaaggaaa ttaatatcct
    41761 gcgcgaaatg cccaccattt aataacaaaa tagtaatgct tactaacagc aatactaaat
    41821 tcagacacat tactgcaata ccgcccaccg cagtaatgcg ggcaatttta ttaatccctt
    41881 ttgaagcaac gacggtgacc agaatcatcc atgccactgc cagcagacca accacctgcg
    41941 taggctccag tccggcaata tgccagtgct gggtcatgtc gctaccgtag aggaatgttg
    42001 agaacggtac ccaaactttc gcggaggtac tcaccatcca gatgatataa gaggaaaacc
    42061 acataaacgt accaatgaag gcaaaacgtg gtccgacact attattcatc caggaataga
    42121 taccgccttc ttctttgcga taagcagctc ccatttcagc catcattaag gcgaatggaa
    42181 taaagaataa caatgcagaa aatatataaa aggaaatcgc actataaccc attaagtaat
    42241 aagccgatgg gctattggca aatccaaata cagaagtaaa gatcatcaat atgagtccta
    42301 tcagactcat cttttttatc gagtgaggca tgaaaccatc cttcatttca gcgcgtgtat
    42361 aaaacgcgcc cactgaactg cactgcccaa cattgagcgg gcagcgtaaa aaccaataca
    42421 gaattgatac ctggatgata tcaaatatcc agtcttaaat tgtggccttt agcggaaaaa
    42481 tgaaaacata tgctacagaa tggcgcttat cacgccattc ttcgctgtta attcagtaat
    42541 ttttcagaat tatgcccggt caacgctaaa ggcgatgact tcagccagtg tctccgcgcc
    42601 cagcgccaac atcaccagac gatcaacacc taatgccacg ccggaacagt caggcatacc
    42661 gactttcaag gcttcaatca gattctggtc aatggggtgc tgcggcaaac cgcgcgccgc
    42721 gcgcttacgg ttatcttgtt caaagcgttg ttgctgctca cgggcatccg tcaattcatg
    42781 gaaaccattc gccagctcaa tacctttata ataaacctca aagcgttcag cgacccgatg
    42841 atcttcggta ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt acacaaaggt
    42901 cggtttttct ttgccaatat ttggctctac gccaaaggta aacagcaatt gtagaagcgt
    42961 gtcgcggtct tcttcagtat cggcaacgtt gctcaaatcc agtttcgctg ccacttcccg
    43021 cagttgcgtt ttgtcggcag agagtgggtc aatttccaga taacgcaaga aagcttgttg
    43081 ataagaaagg ctttctgctg ccggacagtc cagcacctgt tgcaagagat cgtccacctc
    43141 gttcatcaac cggtacatat catagtgcgg tcgataccac tcaagcatag tgaactcagg
    43201 gttgtgataa cgccccatct cttcattacg gaagctgcgg cacagctgga ataccggccc
    43261 acaaccggca accagcaggc gcttcatatg gtattccggg ctggtcatta accagagatt
    43321 catcccctgc gaatgcccgg ggccaacgaa acgtgtctca aacgggacca aatgaatatc
    43381 ggttaccgtc gcctggctca tacaaggcgt ctccacctcc agcactccac gatcggcaaa
    43441 gaaacgacgg atctccgcca taatcgccgc gcgttttaat aagttaggaa tggatgcgct
    43501 cggctgccag gatgccgttt cgctcatagt taaatctcca gtttttgaca agggcacgaa
    43561 gtctactcgc aacgcgacgg cgagacaaat tttacgcagg aataacacaa cggtgggcag
    43621 tgactaaaaa aagcacgatc tgatggttta gtaattaaat taatcatctt cagtgataat
    43681 ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta tccatcagac
    43741 tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta actttcaggc
    43801 acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc
    43861 tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct
    43921 gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata cccgatgcgt
    43981 agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca tgacagcttc
    44041 gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg
    44101 gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg ggggtgccca
    44161 tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag
    44221 cgcacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct gttccagacc
    44281 tctctgcaat tcccgcagat ccagcgtttc gacgaacatt tcgtgctgga tattctagtt
    44341 gatgatggtc atgttcgcgg cctggtagca atgaacatga tggaaggcac gctggtgcag
    44401 atccgtgcta acgcggtcgt tatggctacc ggcggtgcag gtcgcgttta tcgttacaac
    44461 accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca cggcgttccg
    44521 ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc taccaggttc cggtatcctg
    44581 atgaccgaag gctgccgcgg tgaaggcggt attctggtca acaaaaatgg ctaccgttat
    44641 ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa caaatatatg
    44701 gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg taaaggcaac
    44761 accatctcca cgccgcgtgg cgatgtggtt tatctcgacc tgcgtcacct tggcgagaaa
    44821 aaactgcatg aacgtttgcc gttcatctgc gaactggcga aagcgtacgt tggcgtcgat
    44881 ccggttaaag aaccgattcc ggtacgtccg accgcacact acaccatggg cggtatcgaa
    44941 accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct
    45001 gttggtctgc acggtgcgaa ccgtctgggc tccaactcgc tggcggaact ggtggtcttc
    45061 ggtcgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa
    45121 gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct ggttaaccag
    45181 gatggcggcg aaaactgggc taagatccgc gacgaaatgg gcatggccat ggaagaaggc
    45241 tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct ggcagagctg
    45301 caggaacgct tcaagcgcgt gcgcatcacc gacacttcca gcgtgttcaa caccgacctg
    45361 ctctacacca ttgagctggg ccacggtctg aacgttgctg aatgtatggc gcactccgca
    45421 atggcacgta aagagtcccg cggcgcgcac cagcgtctgg acgaaggttg caccgagcgt
    45481 gacgacgtca acttcctcaa acacaccctc gccttccgcg atgctgatgg cacgactcgc
    45541 ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgtgt ttacggtggc
    45601 gaagcggatg cagccgataa ggcggaagca gccaataaga aggagaaggc gaatggctga
    45661 gatgaaaaac ctgaaaattg aggtggtgcg ctataacccg gaagtcgata ccgcaccgca
    45721 tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg cgctgggcta
    45781 catcaaagac aacctggcac cggacctgag ctaccgctgg tcctgccgta tggcgatttg
    45841 tggctcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg
    45901 tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg aacgcgatct
    45961 ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg
    46021 caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga tggcgaagta
    46081 tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg
    46141 cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt ataacgaaga
    46201 tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga acggcgtatg
    46261 gagctgtact ttcgtgggct actgctccga agtctgcccg aaacacgtcg atccggctgc
    46321 ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga ccctgaaacc
    46381 acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca atgacgtcca
    46441 cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc acggcagttc
    46501 cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa aatggcccgg
    46561 aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc attaacctga
    46621 tcactctggc ggcagcccta ctgcacacca aaacctggtt tgagctggca ccaaaagcgg
    46681 ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa agtctctggg
    46741 cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg taaggagcct
    46801 gagatgatta atccaaatcc aaagcgttct gatgaaccgg tattctgggg cctcttcggg
    46861 gccggtggta tgtggagcgc catcattgcg ccggtgatga tcctactggt tggtattctg
    46921 ctaccgctag ggctgtttcc aggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg
    46981 cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct gtggtgtggt
    47041 ttacaccgaa tgcaccacgc gatgcacgat ctgaaaatcc acgtacctgc gggtaaatgg
    47101 gttttctacg gtctggctgc tatcctgaca gttgtcacac tgattggtgt cgttacaatc
    47161 taacgcatcg
//
LOCUS       DAOFSV010000024        46109 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.024, whole genome shotgun sequence.
ACCESSION   DAOFSV010000024 DAOFSV010000000
VERSION     DAOFSV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.024"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(242..859)
                     /gene="tdk"
                     /locus_tag="QBH27_001436"
     CDS             complement(242..859)
                     /gene="tdk"
                     /locus_tag="QBH27_001436"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDQ7013764.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQAYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            1463..1876
                     /gene="hns"
                     /locus_tag="QBH27_001437"
     CDS             1463..1876
                     /gene="hns"
                     /locus_tag="QBH27_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDQ7013765.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2021..2929)
                     /gene="galU"
                     /locus_tag="QBH27_001438"
     CDS             complement(2021..2929)
                     /gene="galU"
                     /locus_tag="QBH27_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDQ7013766.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3131..4144)
                     /gene="rssB"
                     /locus_tag="QBH27_001439"
     CDS             complement(3131..4144)
                     /gene="rssB"
                     /locus_tag="QBH27_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDQ7013767.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4236..5141)
                     /gene="rssA"
                     /locus_tag="QBH27_001440"
     CDS             complement(4236..5141)
                     /gene="rssA"
                     /locus_tag="QBH27_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDQ7013768.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            5254..5712
                     /locus_tag="QBH27_001441"
     CDS             5254..5712
                     /locus_tag="QBH27_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDQ7013769.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGDIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5762..6604
                     /gene="purU"
                     /locus_tag="QBH27_001442"
     CDS             5762..6604
                     /gene="purU"
                     /locus_tag="QBH27_001442"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDQ7013770.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDNLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6764..6848
                     /locus_tag="QBH27_001443"
     tRNA            6764..6848
                     /locus_tag="QBH27_001443"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6798..6800,aa:Tyr,seq:gta)
     gene            complement(6865..7956)
                     /locus_tag="QBH27_001444"
     CDS             complement(6865..7956)
                     /locus_tag="QBH27_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HDQ7013771.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHRYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLIIPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFATTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYSALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKV"
     gene            complement(7943..9067)
                     /locus_tag="QBH27_001445"
     CDS             complement(7943..9067)
                     /locus_tag="QBH27_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HDQ7013772.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNVPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQVFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFCSFGKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(9060..9662)
                     /locus_tag="QBH27_001446"
     CDS             complement(9060..9662)
                     /locus_tag="QBH27_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HDQ7013773.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNLI
                     AFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(9659..10945)
                     /locus_tag="QBH27_001447"
     CDS             complement(9659..10945)
                     /locus_tag="QBH27_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HDQ7013774.1"
                     /translation="MIPLMLIWRYFRTRRLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGKF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDDLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCPCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLELQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(10942..11748)
                     /locus_tag="QBH27_001448"
     CDS             complement(10942..11748)
                     /locus_tag="QBH27_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDQ7013775.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGVVEIRLCHEGDL"
     gene            complement(11726..12742)
                     /locus_tag="QBH27_001449"
     CDS             complement(11726..12742)
                     /locus_tag="QBH27_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ7013776.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEATEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHYDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLIARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(12739..13746)
                     /locus_tag="QBH27_001450"
     CDS             complement(12739..13746)
                     /locus_tag="QBH27_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDQ7013777.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSKQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPSDIDLLICASAIAVQALPCSAI
                     HILKAAAMPVGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLSDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(13874..14551)
                     /gene="narI"
                     /locus_tag="QBH27_001451"
     CDS             complement(13874..14551)
                     /gene="narI"
                     /locus_tag="QBH27_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDQ7013778.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(14551..15261)
                     /gene="narJ"
                     /locus_tag="QBH27_001452"
     CDS             complement(14551..15261)
                     /gene="narJ"
                     /locus_tag="QBH27_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDQ7013779.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(15258..16796)
                     /gene="narH"
                     /locus_tag="QBH27_001453"
     CDS             complement(15258..16796)
                     /gene="narH"
                     /locus_tag="QBH27_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDQ7013780.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(16793..20536)
                     /locus_tag="QBH27_001454"
     CDS             complement(16793..20536)
                     /locus_tag="QBH27_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDQ7013781.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(20929..22320)
                     /gene="narK"
                     /locus_tag="QBH27_001455"
     CDS             complement(20929..22320)
                     /gene="narK"
                     /locus_tag="QBH27_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDQ7013782.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            22659..24455
                     /gene="narX"
                     /locus_tag="QBH27_001456"
     CDS             22659..24455
                     /gene="narX"
                     /locus_tag="QBH27_001456"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDQ7013783.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            24448..25098
                     /gene="narL"
                     /locus_tag="QBH27_001457"
     CDS             24448..25098
                     /gene="narL"
                     /locus_tag="QBH27_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDQ7013784.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(25099..26493)
                     /gene="ychO"
                     /locus_tag="QBH27_001458"
     CDS             complement(25099..26493)
                     /gene="ychO"
                     /locus_tag="QBH27_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDQ7013785.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANHFENSNDGLP
                     DLGMAPEHHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            26678..27031
                     /gene="ychN"
                     /locus_tag="QBH27_001459"
     CDS             26678..27031
                     /gene="ychN"
                     /locus_tag="QBH27_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDQ7013786.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(27075..27770)
                     /gene="chaC"
                     /locus_tag="QBH27_001460"
     CDS             complement(27075..27770)
                     /gene="chaC"
                     /locus_tag="QBH27_001460"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDQ7013787.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27919..28158)
                     /gene="chaB"
                     /locus_tag="QBH27_001461"
     CDS             complement(27919..28158)
                     /gene="chaB"
                     /locus_tag="QBH27_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDQ7013788.1"
                     /translation="MSYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKSSNR"
     gene            28428..29528
                     /gene="chaA"
                     /locus_tag="QBH27_001462"
     CDS             28428..29528
                     /gene="chaA"
                     /locus_tag="QBH27_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDQ7013789.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            29933..30040
                     /locus_tag="QBH27_001463"
     CDS             29933..30040
                     /locus_tag="QBH27_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ7013790.1"
                     /translation="MTLAQFAMIFWHNLAAPILAGIITAVIVSWWRNRK"
     gene            complement(30189..31043)
                     /gene="kdsA"
                     /locus_tag="QBH27_001464"
     CDS             complement(30189..31043)
                     /gene="kdsA"
                     /locus_tag="QBH27_001464"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDQ7013791.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(31079..31888)
                     /locus_tag="QBH27_001465"
     CDS             complement(31079..31888)
                     /locus_tag="QBH27_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDQ7013792.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(31892..32284)
                     /locus_tag="QBH27_001466"
     CDS             complement(31892..32284)
                     /locus_tag="QBH27_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDQ7013793.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(32281..33114)
                     /gene="prmC"
                     /locus_tag="QBH27_001467"
     CDS             complement(32281..33114)
                     /gene="prmC"
                     /locus_tag="QBH27_001467"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDQ7013794.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(33114..34196)
                     /gene="prfA"
                     /locus_tag="QBH27_001468"
     CDS             complement(33114..34196)
                     /gene="prfA"
                     /locus_tag="QBH27_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDQ7013795.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(34238..35494)
                     /gene="hemA"
                     /locus_tag="QBH27_001469"
     CDS             complement(34238..35494)
                     /gene="hemA"
                     /locus_tag="QBH27_001469"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDQ7013796.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEIIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            35708..36331
                     /gene="lolB"
                     /locus_tag="QBH27_001470"
     CDS             35708..36331
                     /gene="lolB"
                     /locus_tag="QBH27_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDQ7013797.1"
                     /translation="MTLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            36331..37182
                     /gene="ispE"
                     /locus_tag="QBH27_001471"
     CDS             36331..37182
                     /gene="ispE"
                     /locus_tag="QBH27_001471"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDQ7013798.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            37333..38280
                     /gene="prs"
                     /locus_tag="QBH27_001472"
     CDS             37333..38280
                     /gene="prs"
                     /locus_tag="QBH27_001472"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDQ7013799.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            38405..40084
                     /gene="dauA"
                     /locus_tag="QBH27_001473"
     CDS             38405..40084
                     /gene="dauA"
                     /locus_tag="QBH27_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDQ7013800.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(40139..40417)
                     /gene="ychH"
                     /locus_tag="QBH27_001474"
     CDS             complement(40139..40417)
                     /gene="ychH"
                     /locus_tag="QBH27_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDQ7013801.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            40695..41279
                     /gene="pth"
                     /locus_tag="QBH27_001475"
     CDS             40695..41279
                     /gene="pth"
                     /locus_tag="QBH27_001475"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDQ7013802.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGSGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            41396..42487
                     /gene="ychF"
                     /locus_tag="QBH27_001476"
     CDS             41396..42487
                     /gene="ychF"
                     /locus_tag="QBH27_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDQ7013803.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            42702..43877
                     /locus_tag="QBH27_001477"
     CDS             42702..43877
                     /locus_tag="QBH27_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDQ7013804.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPTVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAGITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRTISSVFELAVATLRADSDPVWPVRKALPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFNLDNALWTIPAARMKARREHVIPLPF
                     QAVKMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNELGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFK"
     gene            43988..44272
                     /locus_tag="QBH27_001478"
     CDS             43988..44272
                     /locus_tag="QBH27_001478"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7013805.1"
                     /translation="MGTPRFTPEFKEEAVRQVTERGYSVADVSERLGVSAHSLYKWVR
                     SVRPDNSGHQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD"
     gene            44269..45123
                     /locus_tag="QBH27_001479"
     CDS             44269..45123
                     /locus_tag="QBH27_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7013806.1"
                     /translation="MKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHRPVSAGEKDN
                     QRLLELIRDAYALSGGVYGYRRVHGDLREIGEVCSRNRVARIMKRNRIQAVHGYKVPR
                     GVRGRPSLIAPNRVQREFTMVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWS
                     MKPTLSRELALDALLMAVWRRKPAENVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRR
                     GNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRTRRHSHLGGVSP
                     EAFEKASP"
     gene            complement(45746..46109)
                     /locus_tag="QBH27_001480"
                     /pseudo
     CDS             complement(45746..46109)
                     /locus_tag="QBH27_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT        11588 a        12069 c        11341 g        11111 t
ORIGIN      
        1 agcaactttt ccataaaccg gcagcattgt ccttcatcat atttgcgacg atgtataacg
       61 cctacacaca gcgatattgt actttacagg ttacaagtca atgtcggtgt ataagcgctc
      121 tgtgaggcgt ataacggcgt taaaaaactg ccgagaaggg tgtatagccc ggaagaagtg
      181 cgtaaaacga actgacaggg ataaaagcgc cctgctcacc ctgtcagtaa agaaattctt
      241 attaatcgtg gcgatgcctt tcctgaatag ccgttaatga gccgacttct aacgcctctt
      301 tatagtgttt acggcataca gaaacgtagc gttcattacc accaataacc acctgctcac
      361 cttcgttata aggtctgcct gcttgatcaa gacgcagcac catgcttgct ttacggccac
      421 aaaaacagat ggtttttaat tcaaccagtt tgtcggacca tgccagtaag tattgactgc
      481 caataaataa ttcacctcga aaatcggtac gtagaccata acaaagtacg ggtatatcga
      541 gttgatcgac aacctccgat aattcatatg cttgttgtct ggttaaaaac tggcattcat
      601 caaccagtac gcaatgaatt gcctgctgtt catgttccgc acgaatctca tcaaataatg
      661 atgaattttg gttaaataat tttgcaggtg atgacaaacc tatacgcgaa ctgactttcc
      721 cggcaccaaa gcgatcatca atttctgccg tatatacgac agtgcgcatg ccgcgttcct
      781 ggtaattgta tgaagattgc aacaatgctg tagacttacc cgcattcatt gcggaatagt
      841 agaaatatag ctgtgccatc agccacaggc cctcaatgat atgaaataaa gttggctgga
      901 gtttatcata attcgtcagt ttttcagctc aacagtatgc acagaagtgg tatatgcgtt
      961 ctcccttacg aatccttgca taatccttct gagctatcat tacaactgcc tcgcttgtta
     1021 taagcgggta aatgactgcc ggtaactatt cacaatcttt aacctgttgc gcatgtaata
     1081 gccctctgtt gacctccagg agatagtgca atactaagtc cctgctctta ttgcgactgt
     1141 tctacttttc atcattcgct taatagggaa ttctcgtaaa cacgactaat acagaagact
     1201 gaaaggtcgt cagcctacga ttatctcccc ataaaatgtg acatgaatca ggaagtttta
     1261 acctcacgtg ctgcgaaatc atcggtgcaa atagggctat atgccgcgtc ttttctggct
     1321 aattttatga aaagatattt attggcggca caaaataaag aacaattttg aattccttac
     1381 attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca aaccacccca
     1441 atataagttt gagattacta caatgagcga agcacttaaa attctgaaca acatccgtac
     1501 tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt
     1561 agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga
     1621 gcgcactcgt aaactgcagc aatatcgcga aatgctgatc gctgacggta ttgacccgaa
     1681 cgaactgctg aatagccttg ctgccgttaa atctggcacc aaagctaagc gtgctcagcg
     1741 tccggcaaaa tatagctacg ttgacgaaaa cggcgaaact aaaacctgga ctggccaggg
     1801 ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt
     1861 cctgatcaag caataatctt ttgtagattg cacatgctaa aaatcccgcc atcggcggga
     1921 ttttttattg tccggtttaa gacaatttaa taagaataag ctataaaaaa aacggcgtcg
     1981 attgctcaac accgtttcgt ggaaaacacc gatacggatg ttacttctta atgcccatct
     2041 cttcttcaag ccaggcttta aattccgtgc caagggtgtt atgacgaata ccgtattcaa
     2101 cgaaggcctg catgtaacct aatttattac cgcagtcatg gctcttccct ttcatatgat
     2161 aggcttcaac cgtttctttt tcgatcagca tatcaattgc gtcggtgagc tgaatttcat
     2221 caccagctcc aggaggggtt tttgccagca acggccaaat atccgcgcta agtacgtaac
     2281 gacccacaat agcgagatta gacggcgcaa catccgcttt tggtttttca accacgccaa
     2341 ccatcggtac gctttcaccc ggcgctaatt caacgccttt gcaatccaca acgccatatg
     2401 cggtcacatc agcaaccggt tcaaccatga tctggctatg acccgtttca tcaaagcggc
     2461 ggatcatctc tgccaggtta tcctgtgata aatcggattc atattcatcc agaataacgt
     2521 caggcaaaat aacagccaca ggttcatcac ccactaccgg gtgagcacac aataccgcgt
     2581 gtcccaggcc tttcgccagc ccctgacgaa cttgcataat agtcacgtgc ggcggacaaa
     2641 tagactgcac ttcatcaagc agttgacgtt ttacacgttt ttccagcatt gcttccagtt
     2701 caaaactggt atcaaagtgg ttttcaatag agtttttaga tgagtgagta accagcacaa
     2761 tttcagtaat gccagccgca atacattcat tcacgacgta ttgaattaat ggcttatcga
     2821 caagtggcag catctctttc gggatggctt tcgtcgccgg caacatcctg gttcctaatc
     2881 ccgctacggg gataacggct tttttgactt tcgtattaat ggcagccatt taaattctcc
     2941 tggactgttc gtgttttgaa cgtgttcata tttctgtatc gcatcccagt atatcagcac
     3001 cccgaagggg ctaagttctg aaaaggaggc gagttagcat aattaagaca attacttata
     3061 cgaatagcga cgatagtacc accagccgac attagcaggt aatgcaaatt tagcccgtgt
     3121 tatcgtttgc tcattctgca gacaacatca agcgcagccg accaccggtt ccccatattt
     3181 ggcattgcca ggcatcgcat cgctggctca attgattcaa ataagcgtta cctaaagtgc
     3241 ctaacggaac accattactg atttgcacct gatgttcgcc ggtatttaac gtcgcattca
     3301 gacccgcaga aaccagaatg agatttttca gttcgcgatg ataatagcca actaatagcg
     3361 gaaactgccc cggcagattg gcctgacgga gtaaatggtt tacctgcttc aataacgcgc
     3421 ccaactctgg caaccgttga ttttggtgtg caagctgttc ctgtaataat ccgttaaaca
     3481 atgcgcgtaa taacaaggca gcaagtacgc cattatgtcc agctcgggtg acatcaaggc
     3541 aataaaatgc cagatcgttt tccgaaagtg cggcaatatc aagcaccagg ccgggtttgt
     3601 ccgcggcaac taattgacga taattaaccc ggcattggga aatcacctgc tgaaccggcg
     3661 gttgtagttc ctgtaataat ttcgccgctg cagcagggtt atcaaccatt gcatcccagt
     3721 cgcgaaaaag cctttcctct tcctcaacgc gggaattaaa catgctggga tagagacagg
     3781 caaaaaccat ctcgcgcaag cgattcagat ctttaaccgg tttcagcaaa acatcttcaa
     3841 cgcccagacg taacgctttg gcaatatctg ccatattttc agtggcagat atcaccagaa
     3901 ctggtgtctg gtcacctctg ttgcgtatat gttccagcag tttaagcccg ttcattcgtg
     3961 gcatcgcgat atcacatatc atcaggtctg gagtgaaacc tcccagcaac tcaagggcat
     4021 ccaccccatc agccgccagt accgttgtcg ctcccaatga ggaaaaccac gaatccagaa
     4081 gcgagcgaaa tacctgctca tcttcaacaa tgagaatctg ttttccgacc aatggctgcg
     4141 tcatgttctc tcccctgact ggctttactc aatagtggca tgctattgcc acctacgcct
     4201 gtcagaatta acttaagtgt agataaaaat tctggtcaaa tgttggtgcg taccaacggc
     4261 aaaagttcgt ccattttctt ttccactgcc agctgtccgg ccgcaatggc agcgtgcgcg
     4321 cgatggaaat caagcgtaga tatttgcggg caaacaggtt gaatcagaat atcgggcgga
     4381 tcacctgcca tgcggttcct tttaaggcgg ttctccagca cctggataga agtggtcata
     4441 atctctgttg ccgttggcgc tgtcaccgcc cgacgtgtcg ttatgctgcc taacctttct
     4501 ttcagacgcg catgccacgg cagagaatca ccattctcgc tattttcttc actgacatta
     4561 aaggagagca aatcttgttg catcaaatga gcatcgtgct gcaggtcgac cgctatcaca
     4621 atatcagccc ccaatgcacg cgtgagggaa ataggaattg ggttaactac agcgccatca
     4681 accagccagt agccgttatg cgcaacgggt gccattagtc ctggaatact gcatgatgca
     4741 cgaatagcaa gatggagatc gccttcagta aaccataatt cacgcccagt acttaaattg
     4801 gtggcaacag ccgcgaagcg acgggaacaa ttttcgatct ctgtttccgg cattatttcg
     4861 cgatattgat tgaagacacg ctcgccgcgc agtaacccac cgcgctgcca ggagagatcc
     4921 atcaggcgta aaacatccca ataactgaaa gaggtcaccc aatcttccag cgcagataat
     4981 cgatcgcatg cataggcagc gcccaccagc gaaccaattg aacatcctgc aacgatatca
     5041 atttcaatac ccactttttt tagcgcatta attacgccaa tatgcgacca acctcttgcc
     5101 gcgccagatc ccagcgccag ccctattttt atctttctca ttattcccgc aagattcccc
     5161 tggaatgcaa tcgtagccac atcgctactg ctcggttaac atagtgctac tcaggcggtt
     5221 aatacgccgt tattgtttcc agggagatta tttgtgtctc agctttgtcc ctgtggtagt
     5281 gctgtcgagt atagcctatg ttgccaccct tatgtgtctg gtgaaaaggt tgcacctgat
     5341 ccagaacatc tcatgcgttc gcgttactgc gcttttgtga tgcaagacgc agattattta
     5401 ataaagacct ggcatccatc ttgtggagca gcggcgttac gtgccgaatt gattgccgga
     5461 tttgcgcata ctgaatggtt gggattgacc gtatttgaac attgctggca ggacggcggc
     5521 gatatcggat ttgtcagttt tgttgctcga tttaccgagg gtggcaaaac aggtgcaata
     5581 attgaacgtt cgcgattttt gaaagaaaac ggtcaatggt actatattga cggtacacgt
     5641 ccgcagtttg gccgcaacga tccctgccct tgtggttcag gtaaaaaatt taaaaagtgc
     5701 tgcggccaat aatggttgac ggtacggttt agcaaacact ctcaacaagg tttttccagc
     5761 aatgcattca ctccaacgta aagttctgcg tactatttgt ccggaccaaa aaggtctgat
     5821 cgcacgtatt accaatattt gctacaagca cgagttaaat atcgtgcaga acaatgaatt
     5881 tgtagatcat cgtaccgggc gcttttttat gcgcacggaa ctggaaggga tttttaatga
     5941 ttccaccctg ctggcagatc tcgatagcgc attgccagaa ggctccgtgc gtgagctgaa
     6001 tcctgccggt cgtcgccgga tagtgattct ggtcactaaa gaagcacatt gccttggcga
     6061 tttgctgatg aaagccaatt acggcggcct ggatgtcgaa atcgcggcag ttattggtaa
     6121 ccacgataat ttacgttctc tggttgagcg ttttgatatt ccgtttgagc tggtaagcca
     6181 tgaagggtta agccgcaacg agcacgatca aaagatggcg gatgccattg atgcttatca
     6241 acctgactat gtggtgctgg cgaagtatat gcgggtatta acgccggaat ttgtggctcg
     6301 cttcccgaat aagatcatca atattcacca ttccttcctg ccagcgttta tcggcgcacg
     6361 tccttatcac caggcctatg agcgtggcgt gaagattatt ggcgcaaccg ctcactatgt
     6421 gaatgacaat ctggacgaag gtccaatcat catgcaggac gttattcatg tcgatcatac
     6481 ctacacagct gaagatatga tgcgcgcagg tcgtgacgtc gagaaaaacg tcttaagtcg
     6541 tgcgctatac aaagtactgg cgcagcgcgt ctttgtttac ggtaatcgga cgattattct
     6601 ttaatcgtca gccaaaataa ctggttagct ttaatccgtt acggatgaaa atcacgcaac
     6661 cagttcattt ttctcaacgt aacactttac agcggcgcgt catttgatat gatgcgcccc
     6721 gcttcccgat aagggagcag gccagtaaaa agcattaccc cgtggtgggg ttcccgagcg
     6781 gccaaaggga gcagactgta aatctgccgt catcgacttc gaaggttcga atccttcccc
     6841 caccaccatc actcgattcc attatcaaac cttcggcttc tcccccattc gccgtaaaaa
     6901 ctgctgctgt agcctcaaca aatccgctaa cgtcaacaac tgataatcca gaccttgctg
     6961 gctggcaatt tgcgcaataa tgcggcttag cgccgaataa tggcgatgat gccagtgagg
     7021 aaacagatga tgggtaaggt gcagattaat cccacccagc cagtagccaa gccatgcagg
     7081 ttgcggcgtc caatcaaagg tggtagcaaa ggtatgcgcc agtctgccca cgggcatttt
     7141 gccctcttct ggtggtaact gcgtatgtcc cttcgcccag tgcgttccaa taatcaacgt
     7201 cacaaaaatc aatgacgcca gcagctggct gagcagataa gtcagcagga tggtcataaa
     7261 actaaaatca gacaaaagcc agggaattat cagacacagc gcacaatgcg ccagtttccc
     7321 ggctaagaaa tatccccagc cagcaaagcc tcgcagggcc agatgtcgcg tgaccggcgt
     7381 aaaccctgcg cgatcgagcc agtccactac ccagatatac cagggaaagg tgagcgcggc
     7441 gaccagtggc caatagcgat gttgctggcg cataaaaggt cgccaccgtt gccacggcgt
     7501 ctggcgaaga atgccatttt ctgctgtatc tggatcgtaa aattcaatat tggtaaaacc
     7561 gtgatgaaag cgaacgtggc gcacccgcca gcagtccgga tctagcccca taggaaaagc
     7621 aacccaccgg ttaagccggg cattggtctt tttaccacgc aaaaacgcat catgcgaagc
     7681 gtcatgcacc acattgaccg ctaacaacat ggcgcaaaac atcatcgcga gataccacaa
     7741 cagatacagt ccccattgac gttgaaataa cgccagtaag taacaggaga aacaagcaat
     7801 aatcaacacc gccattttga cgaatgccca tccgtcggca taccgatgat cgcctgattt
     7861 cgccagataa actttcgtac cgctttgcag ttgtttcacc agttgtggat catcggcagg
     7921 aaaagtgatt aacctgtcgg gattaaacag gttcatactg tttccttagc gatttacgtc
     7981 ctacgccgat tacaaataac aggcaatggg ccagcagaat acccactaat cccttccaga
     8041 gcagagtact ccatgaacac atatagacaa taaaatagag cagtgacaac gtcacagagc
     8101 accacaaaat cataccccaa tgctgcccgt cgctcatgcc ccccagcgca acggagcctg
     8161 ccgccagcag aacaaataat acggcttgct cgctggtaat aaactcaatg ccatagccat
     8221 attgttgaat gtagccaagc accagcataa acagcaggat cacccctgcc cctaccgccc
     8281 atggagaact ctggtagcga ccttcaatcg ctttgccaaa tgagcaaaag cccattaagc
     8341 gtaaaaatgg cagattactc tcgcgcatcg ggttacagga caagcgacta ttttttacgc
     8401 cgtagcctac gtcgtattcc ggcccggcac tatacgaacc gaaaagttta tcccacaaca
     8461 aaaaggtgcc accgtaattg gtatcggcat attgcttttg attcagatga tggacgcgat
     8521 gatgcgaagg cgtgatcagc actttttcca gccagcccag tttcggcgtt agtgcgttat
     8581 ggttaaaaaa ttgccaacta tagtgaataa tagaaaccgc caaaaagacc tgtaacggca
     8641 caccaagcat cgccagcagc ataaaaaacg ggattgaggt taacgaggag taccacgagt
     8701 tccgcacgcc taatgacagg ttgtagtgtt ccccctgatg atgcaccaca tgtaccgccc
     8761 acagcgggcg catttcgtga tgccagcgat gtagccagta aaagccgaaa tcccacgcca
     8821 ggatagtaaa caaccacagc aggatcaccg gcacattatc aaacagtcca aggctgaagt
     8881 gattgaccac caggccatag cagagcactt ccaggcagcg aaataaccac agcatcatat
     8941 gaccggagtt gatattaaaa ataacgtcca gccagtccac tttttgccgc ccggtgaatt
     9001 gcaacacgcc cgcttcggct aacaccaaca gcaccatcga tatgatgggg aataataaat
     9061 cactcatatt gtgtcttcct tttctcgcat gttgcgcata tccaaccgca aatcgacgcc
     9121 agcaacgcgc cgcctactaa atcgataaat aaatgtctgc gtaattgcag aatcgaaaag
     9181 gcgattgcta tcgcccagac cacgctcatt agcgtcagcc aacggttccc ctttttagca
     9241 atcgcccata ccgccaacag cgttagcgcg gcgtgtaagg agggaaaaca gttctggctt
     9301 gagtcaatgg cgatcagcct gttgagtgcg cccgcggata tccccatctg atcaaacgag
     9361 ggataatgca tggttgtggg ccagagaaga taaaatacgc cagccaccag tgcggaaatt
     9421 tgcattgaac gggccagcca gcgcaggtgg caaagtggcg tggataaata acctaacggc
     9481 acaatgaaaa aaaacgagag atacagccac acagcggagg gggaaaacgc aatcaaatta
     9541 tcaattattg acggtgttaa ttgatatccc tcaccttgca ggtagtcgct aaagttgtag
     9601 ataaccccaa cagttcccca gcccagcagc atctgtttca ggcgtagcag tgaattgctc
     9661 attcccgccc ccactgacgc acaatgcgcc gccgcttggc tgtaaaagag actggcacag
     9721 tttgcagttc cagcgaccaa attaattgcc cgacagcgat atcctggcgt ataaacaagg
     9781 cattcaacgc ctcccggcaa tgctccagca tccacagctc gcaatcggcg attaacgtca
     9841 attgtgctga cccggtttgt agcaggcgat aatcgactgt tagcggcagt gtcattgcca
     9901 gcacacgact acaggcatcg gcaaaaacgg tctgaatatc gccggatctt gttggtagtt
     9961 gcagttggtc atcctgccgc ccttcgatac gcgcaatcgc cattgttgca ctgccacagg
    10021 ggcatggctg ttcgcttgcc accagcacat catccagtcg ataacgcact atcggctgtg
    10081 tggtgcgggt aaagtctgtg acgatcggca cgaaacggtg ctcatccagc cattgcggtt
    10141 caatgtgaac aaactcttcg ttgagatgta acgttccaca ccggcaggtg gaagcgagaa
    10201 agccttctgt cgcctgataa atttccccca cattgtggaa cacgttgcgc agtaattccc
    10261 gatcctgcgg ctccagaact tccgccaccg aaatcacttt tttgaccttt agcgtcaatt
    10321 cgccggccat cacctccagc gccagtgcgc gtaatacctg agctggcgcg acaataatcg
    10381 tgggcgctcg ctgttggaga agtttgagtt gcgcctgaaa tggcgctagc agatcgtaaa
    10441 aatcgagact taaccagcga ttattcacgc tttgatacag gttgttgtcc gcgcgcagaa
    10501 acagcgccac ccgctctttg gcaaataaat catcgggcaa cacttttgcc agcacaccgg
    10561 cggcccacat ctgttgctcg tgcgggctaa caacaaaaag tccacgacgc ccggatgtac
    10621 ctgatgaaag cccaacgctg aacttaccaa cgcagggttt gaaatcacga ctctgctcac
    10681 tgcgcattgc acaatcgaga agttcgtcgc gctttaaacc tgcggtattc atctcatcga
    10741 agtgcgtcat cattaacgct ttatccatca ttggccactg attaaagggc aacgtcagat
    10801 aacgctgaaa ccacgggcta tgagataaga cattttgccg gaactgatgc agcctttttg
    10861 cctgccagtt ttccagtgcc tcgcgatcgg tgaaatgcag cctgcgggta cggaagtagc
    10921 gccagatcaa catcaacggg atcataaatc cccctcatgg cacaaacgaa tctccaccac
    10981 accgttttgc caaagctgat ggagttgccc aagcgtgtgg tagtaggcgt ggctgtcgga
    11041 cataaccaga tgcgccagtg gtgaaggccc tcgcagctcc cggtagttag ctggcgccca
    11101 tgccgcatcg ccagccagta aagtccagcc gttgtcggtt aacacaaacg cgccaatatg
    11161 tccggcagca tgtcctggaa gcggcacgag aataatttcg ccattgcttt ctggcagcaa
    11221 atagccgcac tcaaacggtg ccagttgcgc gggcaaattc accgatgcga aactttcaat
    11281 aaagcgcagt gaggagtcaa aattctccgg gattagccct ggcacaaagg cctggcgcag
    11341 cgcagtgaca ccacgcagag ttcttacttg ccgccagcct tcaccagaac agatgcagga
    11401 aacatcaggg aaatcacgca ggccagcaat atgatcagcg tgaaaatgcg acagaataat
    11461 cgcatcaaca tctttcgctg cgaggccggt ggcagaaagt tgtcgcacca gcgcctgttg
    11521 cggatcgaaa taaaccggcg tgacctggcg ataaagacga aacacaccag attgcgtgta
    11581 gtgttcgaac cacgatgaat agcctgtgtc ccacagccac cgtttgtcgc ccacctccag
    11641 cagccaggcg cgggcgggga atttgcacac tttcagccct gcccctttca gcgcaataca
    11701 gctaatatgg gtacaccagc ccgtttcaaa tgcggttatt tttgccatgt tccctcagcc
    11761 attgtcccgt gattgcaata ccttgttcca tcgaaaagcg tgggcgatat cccagctctg
    11821 taatggcctt ttgcgcactc agcgtcatat cgaaacagag tgttccggcg ctgtatcgtg
    11881 tgataacagg ttcctgatta acgcacttcc catacagttc caggccacgc gcgatcagag
    11941 aaagcagcga ccagggaaca gcagcaatgc ggtaactcaa gcccagttgc tggtgcagca
    12001 aggcgtcaag catcaccgat agctgctgcg gctggtgatt gctgatgttg taaatactgc
    12061 ccgagcgtaa gccttcaacg ttcgttgcca gccgcatggc atacaccacg ttttgcacga
    12121 acgtgagatc caataacgcg tcgcccccgc gcggcaaacg taagcaaccg ccgccctgtt
    12181 gcaattgctg catcagacga ggaacaatta cctggtcatg ggggccaaac aaaccacggg
    12241 gacgcaaaag aataaatctc gtggcagaat atcgagaaac agcgccagca ataacctgct
    12301 cagcggcata tttactgctg gcataatggc tggagaatgc tcgcgcaaga taagtttctg
    12361 gcaggtcata gtgatgttga aaatcaaagt aaaccgcagg cgtggagata tggataaacc
    12421 ggggaatatt cagcctgcct gccgcctgtg ccagagtgtg ggtcactttg acattggcaa
    12481 gccagaacgc cgttttgtcg ccccagggcg aggactttgc cgcacagtgc cagacggcat
    12541 cgcagccgtc catcaggcgg gcgcagtcat cctcggttgc ctcggttaaa tcgagagcca
    12601 caaactgtgc gcctgcgttt gtcaggcttc tgccaacctc acaatttcgc cctgttgcca
    12661 caaccgactc acccgattgc aacaaatatt gcacggcatt tcgcccaagg ccgctggtcg
    12721 cccctgttac gaggatcttc acggtaaaag caccatgcca gccagcgtta gtcccgctgc
    12781 cgtgccaatc aacattacag gtttatccgc tgtgaaacga ccgctaatca ctgcttcatg
    12841 cagcgccgtg gggatagagg ccgcgacctg attgccgtaa aaacgataca catcgatgag
    12901 cgcagattcc ggcactcgca gccgtttacg catatgttca agggaaagat gactggcctg
    12961 atgcggaacg accgtgccga tatcagacag ttgcaggctg cttttttgca gcaaccgaga
    13021 aaaataatct tcaataagcg aagaagcaaa acgaaacagc ggtttcccct gcatatgaaa
    13081 aagaaagtcg ctatcactca caccagagcg aggattacgc cgcgtaccgc cagcacgaat
    13141 ttcacacagt tcgctgcctt cgggatacat ttcgatcagg ctagcgataa tcccgctgcg
    13201 cccatcgccc cgctcaacaa tcgcgcacgc cgcgccatca ccaaaaatta atgaagactc
    13261 ttcatgatcc cagtcgatac cgcgtgaagc aatatctgcc gaaacgatgg caatgcgtcg
    13321 atatgctcca cagtttaata atccggcagc gacttcaaga gcggaaataa agctcacaca
    13381 gctactgttg atatcaaacc cggcaacgcc tacgggcatg gctgcggctt taagaatatg
    13441 aattgcgcta cagggtaatg cctgtacggc aatggccgat gcacaaatta gcagatcaat
    13501 atcagaagga tttaaggctg ccgctactaa cgcattatgt aaagcctctg ccgccagttc
    13561 ggcctggctg gcgtcgttgc gggcaaaata gcgccagcaa ataccggaac gtcgctcaac
    13621 atagccaact ggcttattta actgaacatc cagttgttta gaagtgattt gatcaggggg
    13681 taaggcgatc cccgtggcga ttatttttaa cgccgcggag atcgtcggtt gattaggtcg
    13741 agccacggtc agtgctcctt cactgctcca taggtggacc aatcatatag atgaagtacg
    13801 tttccctaca atccgaaaaa tcggcagtgt gtaaccgccg atgtttggtt tagataatgt
    13861 ttggtttaga taattagtga cgagcgcgca ccagctgata cttacgcgtc agatactcca
    13921 ccgggacact ccagatgtgt accagacgcg agaacgggaa cagcaggaat aacgtcatcc
    13981 ccaacaccag gtgcagacgg aagataaacg ccacgccatc aagatgttga gaagcgccac
    14041 cgtggaaggt cactaccgac tgcgcccagc caaccagttt catcatctcg ctaccgtcca
    14101 tatgctgagc agagaacgga atggtcagca ggcccagcgc gcactggata acgagcagcg
    14161 acaggatcag gatatccgct ccggtagtcg ttgcacgcac gcgtgggctg aacagacggc
    14221 gtttcaacaa cagcacgccg ccaatcagac acagtacgcc gctggcacca ccagcaaaca
    14281 tcgccatttt ctgcttcact tcaatcggca gccaggcttc atacatccag tgcggcgtca
    14341 gcataccgaa gaagtgaccg acaaaaatcc ccagaatacc gatatggaac aggttcgacg
    14401 ccagattcat ccctttgcga tccagcatct ggctggacgc cgcgcgccag gtatactgcc
    14461 cgtagtcata acgcagccag ctaccaatca ggaagaccgc cccggctatg tacgggtaga
    14521 tatcaaagaa gaacatattc aggaattgca ttagtgctgt cctccggtgg tgatattcag
    14581 atattgcggc gcgacggcac cggcaaagcg acgctgatga gcagtgattg ctgaatcacc
    14641 gcagcctttg tcagcaaaga atttaacctg ctcttcttcc cagactgcat ccagcgcctg
    14701 cggcgtatcg tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc tgtcgattgc
    14761 ggtattcgcc agtttcagca gcagatcaaa cagcacggca taacggcttt cacgctgttg
    14821 cagacgcgca ctcagcaatg ccagaatcgg cgcaatatct ttcaaaccct ccacggcttc
    14881 gctttgcggc agctgcgcca ggtactccag atacagcggc agatggtcgg gcaattcgcg
    14941 gctgtttaac tgcaagccgt gctgctcgta ctgcgccagc agatccacca tcgcctggcc
    15001 gcggtcacgg gattcaccgt gaacatgttc aaatagcaac agtgacgtgg cgcggccacg
    15061 gtcgaacagt tcgctgtact gcgcctgggc atcgagcgga tccatcgtcg ttaaatcgcg
    15121 caggaaaatg cccagcgcat gggcatcctc ttttggcaga tttttcgacg cggcaatcgc
    15181 ctcaaacatc tcttgttgat gctgccataa ggcagcatcc ggatattcaa ggagacgcga
    15241 tacaatcacg agttcgatca tgggtgcggc tccgttttgc tggttacgtc gatggcatcg
    15301 atacggcggc tgttgaacag attgaatttg gtatctgaac cgtggcagcc atcaccaaag
    15361 gtaaagccgc agccattttt ctccgggaag gcttcccgcg ccagttcacg atgactgctc
    15421 ggcaccacaa agcgatcttc gtagttggca atagccagat aacgatacat ctcctgtgcc
    15481 tgggcttcgg tcagaccgac ctcttccagc gcacgggtat cgactttacc gtcaacggtt
    15541 tcagcacgtt tgtaatgacg catcgccagc atacgtttca gcgcgcgcag taccggtttg
    15601 gtatcaccgg cggtcagcag attcgccaga tactgtaccg gaatacgcag gctttcgacg
    15661 tctggcagaa tgccgttgct gcccagttca cccgcgtctg ccgcagactg aatcggcgac
    15721 agaggcggca cgtaccagac catcggcaat gtgcgatatt ccggatgcag cggcagcgcc
    15781 agtttccatt ccatcgccat tttataaact ggcgactgct gcgcagcctc aataacgctc
    15841 agcggaatac cgtctttaat cgcctgctcg atgactttcg gatcgttcgg atcgaggaac
    15901 acatccagct gacgctggta gagatctttc tcgttctcgg tacttgccgc acgttcaatc
    15961 gcgtcggcat cgtacagcag cacgccaaga taacggatac gaccgacaca ggtttctgaa
    16021 catattgtcg gctggccggc ttcaatacgc ggatagcaga agatgcactt ctcagactta
    16081 ccgctcttcc agttgaagta gatttttttg tacgggcatc cggtgatgca catacgccag
    16141 ccacggcatt tatcctggtc gatcaggacg atgccgtctt cttcacgctt gtaaatcgca
    16201 ccgctcgggc aggtcgccac acatgccggg ttcagacagt gttcgcacag gcgcggcaaa
    16261 tacatcatga aggtgttttc gaactggcta tacatcgcct tctggatgtt gtcgaagttc
    16321 ttgtctttcg ccagcttgtc aaactcaccg cccaggtcat cttcccagtt cggccctttt
    16381 tcgattttcg ccatccgttc gccggtaatc agcgaacgcg gacgggcaat cggctgtgat
    16441 ttgctgcctt ccggcgcggt atgcaggttc tgataatcaa aatcaaacgg ctcgtaataa
    16501 tcgtcgatcc ccggcagatg cgggttagcg aagattttac ccagcagcat ggcacggtta
    16561 cccatgcgcg gctgcagttt gccgttgatt ttacggatcc agccgccttt gtatttttcc
    16621 tggttttccc agtcagtcgg gaagccctgg cccggcttgg tttccacgtt gttgaaccac
    16681 gcgtattcca cgccttcacg gctggtccag acgtttttac aggtcactga acaggtgtgg
    16741 cagccgatgc acttatcgag attcagcacc atgccgactt gtgaacgaat tttcatttta
    16801 cgctctcctg tacctggtca ttgccttcgc catctaacca gtcaatgttc ttcatcttac
    16861 gcactacaac aaactcatcg cggttagaac ctacggtgcc gtagtagtta aagccatatg
    16921 ccagatgggc atagccgccg atcatatgcg tcggtttcgg cgtgatacgg gtgaccgagt
    16981 tatggatacc accacgctgt tgggtaattt ccgaaccagg caggttaacg atacgttcct
    17041 gcgcgtggta catcatggtc atccctgccg gaacacgctg gctgacaacc gcacgggcag
    17101 tcagtgcacc gttgctgttg aagacttcaa tccagtcgtt atcggcgata cccagctctt
    17161 tggcatcggc ttcactcagc cagaccaccg gaccaccgcg acctaaagtc agcatcaaca
    17221 ggttgtcgct gtaggtggag tggatacccc acttctggtg tggcgtcagg aagttgagcg
    17281 ctttttccgg gttgccattg gatttctggc ccatcacttc tttcaccgaa cgggtgtcga
    17341 tcggcggacg ataaaccagc aggctttcac cgaaatcacg catccattgg tgatcctgat
    17401 acagctgctg acggccagag agcgtacgcc acgggatcag ctcgtgaacg ttggtgtaac
    17461 cggcgttgta agaaacgtgt tcatcttcaa ggccagacca tgtcgggctg gagatgattt
    17521 tgcgcggctg tgcctgaata tcgcggaagc gaatcttctc gtcttcttta ttcagcgcca
    17581 gatgcgtatg gtcacggccg gtaaactcgc tcagggcagc ccaggctttt acagctacct
    17641 gaccgttggt ttccggtgcc agcgtcagga tcatctctgc cgcatcaatt gcggtattga
    17701 gcatcggctg accttttgcc ggaccttccg cttttgtgta gttgagctta cgcagcagat
    17761 ccatctcgct ctgggtgttc caggcaatcc ctttaccgcc gttaccgatt ttctccatca
    17821 gcgggccgat agaggtaaag cgttcgtaag ttgccggata atcgcgctct acgaccataa
    17881 tgtgcggtgc ggttttacct gggatcaggt cgcattcgcc ttttttccag tctttcacat
    17941 ccagcggttg cgccagttca gcggcagagt cgtgctggat aggcagcgtg acgatgtcag
    18001 tttctttacc cagatgacca acgcacactt cggagaattt cttcgcgatg gctttgtaga
    18061 tttcccagtc gcttttcgct tcccaggccg gatcgaccgc cgcagacagc gggtgaataa
    18121 acggatgcat atccgaagta ttcatgtcgt ctttttcgta ccaggtcgcc gtcggcaaaa
    18181 tgatgtcgga atagagacag gtgctcgaca gacggaagtc cagcgtcacc accagatcca
    18241 gcttgccttc cagaccgttg tcctgccagt ccacttcttc cggcttcacg ccgccctgct
    18301 gccccaggtc tttgccctgg ataccgtgct ccgtccccag caggtacttg agcataaact
    18361 catgaccttt accggaagaa ccgagcaggt tagaacgcca gatgaacagg ttacgcgggt
    18421 ggtttttacc gttttccggc tgttccgcag cgaaacggat agaaccctct ttcagggatt
    18481 tcaccgtata gtccaccgga ttcatcccgg ctttttctgc ttcgcgagcg atagtcagcg
    18541 ggttagtgcc taactgcggc gcagacggca gccagcccat gcgttctgca cggacgttaa
    18601 agtcgatcaa gtgtccggta tagcgagatt tatccgccat cggcgacagc agttcttccg
    18661 cagtcacggt ttcataacgc cactggctgg agtggttata gaaataagag gtactgttca
    18721 tgtgacgcgc cggacgctgc cagtcgaggg caaacgccag cggctgccag ccggtttgcg
    18781 gacgcagttt ttcctgacct acatagtgcg cccagccgcc cccgctctga ccgacacaac
    18841 cgcagaaaat cagcatgttg atcagaccac gatagttcat gtccaggtga taccagtggt
    18901 tcagccctgc gccgacgata atcatcgaac gaccgtgcgt tttatcagcg ttatcggcaa
    18961 attcacgggc gatgcgaata atctggctgc gagaaacgcc ggtaatctgc tctgcccagg
    19021 caggggtgta agctttcaca tcgtcatagc tggttgcaca gttaacgtcg ttcaggccac
    19081 gttccagacc gtagtttgcc agcgtcagat cataaacggt ggtcaccagg gcggtgctgc
    19141 catcagccag ttgcaggcgt ttcaccggca gcttatgcag cagcacgttt tccagttcca
    19201 ctttgttgaa gtgttccgtg ccatcgccac caaagtacgg gaagcccacc tcagcgatct
    19261 catcctggct acccagcagg ctcagttgca gctcggtttc ttcgccagtt ttgccgtcgc
    19321 gctgctcaag attccatttg cccttctcgc cccagcggaa gccaatagaa ccgttcggcg
    19381 caaccatttc accattggta ttaaaggcga cagttttcca ttccggattg ttttcctggc
    19441 ccagtgcatc caccagatca gcagcgcgca gcatacgacc cgcagcgtag taaccgtcgc
    19501 gctcttccag catcaccagc atcggcatgt cggtgtagcg gcgcacatag tcggtgaaat
    19561 actggcttgg gttgtcgagg tggaattcac gcagcattac gtgacccatt gccagcgcca
    19621 ttgccgcatc ggtgccctgt ttcggtgcca gccacagatc gcacagtttg gcgatttcag
    19681 cgtagtctgg tgtgacggca acagttttgg tccctttgta acgcacttca gtaaagaagt
    19741 gagcatccgg ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg tagctggagt
    19801 tgtaccagtc agcagattcc ggtacgtcag tttgctcacc ccaggtttgc ggagacgcag
    19861 gaggcaggtc gcagtaccag tcgtagaagc ttaagcaagt accgccaatc agcgagagat
    19921 agcgcgcacc cgatgcgtaa gaaaccatcg acattgccgg aattggcgag aagccagcga
    19981 cacggtccgg gccgtaattt ttgatggtgt aaacgttaga tgcggcgatc agttcgttca
    20041 cttcctgcca ggaagaacga acaaaaccac cgcgtccacg cgcctgctta aagcttttcg
    20101 ctttatcggc gtcttcaatg atagaagccc atgcctcaac cggatcgcta tgcagcgcct
    20161 tcgcttcacg ccacattttc atcaggcgtt tgcgcatcat cgggtatttc aggcggttag
    20221 cgctgtaaag ataccaggag tagctggcac cgcgcgggca gccgcgaggt tcatggtttg
    20281 gcaggtccgg gcgagtacgc ggatagtcag tctgctgggt ttcccaggtg accagaccgt
    20341 ttttgacgta gattttccag ctgcaggagc cggtgcagtt taccccgtgg gtagagcgga
    20401 cgattttgtc atgctgccaa cgctggcgat atccatcctc ccagtcacgg ttggtattga
    20461 gaagctggcc atgcccatcg gcaaaggttt cacccttctg tttgaagtag cgaaaccggt
    20521 ccaggaattt actcatcggt tttctcctgt gggagcctgt cggccctctg ataaatcgac
    20581 attgctaatg tttaagggca aggtaacgct ctgaaacagc gtgggaattg ataacgatca
    20641 agagtgatgg ggatgaaaaa taaagtaaat tcctggaata cccctttagg ataattaaca
    20701 tttatagcat aacagactgt aaattaagac gttttacacc tgtggttatt taaaccatta
    20761 agatttttat gcagatgggt attaaggagt attccccatg ggtaacatat taatgactct
    20821 taatgaaatc cagcgaaata agccatacaa cattagtatg ttgtaactaa atgacgcata
    20881 aaaaaagcgc gacatcatgc cgcgcaaagg ataatcaaat attacgattt attttttaga
    20941 atgccgacca tataccgccc aggtaatcac cacgcaggcg atatagaaaa tcaaaaatac
    21001 tttcattgcg ccgactggcg aacccgttaa tgccagcgag ctaccaaacg ctttcgggat
    21061 aaagaagcca ccaatcgcgc caatcgcaga gatgaaaccc agcgccgccg ccgtgtcggt
    21121 tgccgcttca cgcatcgcac gttcgtcaga acccccttct gctttcacgc gatccattgt
    21181 cagtttacgg aagatcacgg aaatcatctg gaaagtggaa ccactaccca gcccagcggt
    21241 caggaacagc gcaaggaaga ctgcgaagaa cgccatgaag cttccgccct gtccgtcagt
    21301 cggtaaggtc aggaacagca ggccgctgaa aatcgccatc aggataaagt tcaccagcgt
    21361 gacacgagtt ccgcccagac ggtcagataa tgcaccacct gcagaacgcg ccagcgcacc
    21421 aataaacggc ccgaagaaag cgtattgcag aatctgaaca tccgggaact gcgtttttga
    21481 cagcatcgca aagcccgcgg agaagccgat gaaggagccg aaggttgcca gatacagaag
    21541 gctcataatc cacagatgac cccgtttgag taccggcaac tgctccttga tggaggcttt
    21601 cgaggtagca agatcgttca tgccaaacca cgccgcaatg gtgaagatgg caaggaacgg
    21661 cacccatatc caggacgcat tcgccagata cagctcagtc ccatccggct gtttgacgcc
    21721 ctggctacca aatactgcga aaatcgacag tgataccacc agcggagcaa ccaactgcat
    21781 gacgctgacg cccatgttgc ccagaccacc attcagaccc agcgcgccac cctgcttctg
    21841 tttcggaaag aagaagctga tgtttgccat actggatgcg aagttcgcgc cagcaaagcc
    21901 gcacagcaga gaaatgatga tgaagacgct ataaggcgtg gaggtatcct gcacggcaaa
    21961 acccagccag acgcaaggaa taatcagaat accggtgctg aacgccgtcc agcgacgacc
    22021 accaaagata ggaaccataa aggagtatgg aacacgtaat aacgcgccag aaaccgaagg
    22081 cagcgcagtc aacataaata gctgatcggt cgtaaaatta aaaccgactt tcggtaagtt
    22141 caccgcaaca gcgctgaaca acatccatac gcaaaacgcc agcaacagac agggaacgga
    22201 aatccacagg ttgcggctgg caatacgttg accgcgttgt tgccagaacg caggatcttc
    22261 cggtcgccaa tctgtaatga cagctccagt agccctttcg ggggcggatg agtgactcat
    22321 agacacctct gatactcgtt tcgatgtctg ccaccttagt gtctgtagct aaaggcaatt
    22381 tgatgtaaat caaacgataa gataacttta tctatcattg atatttatca ttatccatag
    22441 tgagtacagt gatttcataa aaattatgag atttttcacg gtactgtaaa atccctaccc
    22501 ttaccgatgt aaagcgacta accacacggc aaataaggag taactctttc cgggtatggg
    22561 tatacttcag ccaatagccg agaatactgc cattccagaa tgtatcgtca cattcattaa
    22621 ggttattgct catttaaagc ctgaaggaag aggtttacat gcttaaacgt tgtctctctc
    22681 cgctcaccct ggttaatcag gttgcgctta ttgtgttgct ttctactgct attggactgg
    22741 cagggatggc ggtttctggc tggctggtgc aaggcgttca gggcagcgcc catgcgatca
    22801 acaaagcggg atcgctgcgc atgcaaagtt accgtctgtt ggcagcagtg ccattaagcg
    22861 agaaagacaa gcccttaatt aaagagatgg aacaaacagc atttagcgcc gagttgactc
    22921 gagcagcaga acgagacggg caactggcgc aattacaggg tttacaagat tactggcgta
    22981 atgaactgat ccctgcactg atacgtgcac aaaaccgcga aacggtgtca gcggatgtca
    23041 gccagtttgt tgccgggctt gatcagctgg tatctggttt tgatcgcacc acggaaatgc
    23101 gcattgagac agtggtgctg gtccatcggg taatggcggt atttatggca cttttactgg
    23161 tattcactat tatctggttg cgggcgagat tgctacaacc gtggcggcaa ctgctggcaa
    23221 tggcgagtgc cgtcagccat cgcgatttta cccaacgcgc aaacatcagc gggcgcaacg
    23281 aaatggcgat gcttggaact gcgttgaaca atatgtctgc agaactggcg gaaagttatg
    23341 ccgtacttga gcagcgggtt caggagaaaa ccgctgggct ggagcataaa aatcagatcc
    23401 tctctttttt atggcaggct aaccgccgtt tgcattcccg cgccccgctg tgtgaacgcc
    23461 tgtcacctgt actcaacggc ttacagaatt taaccctgct acgtgatatc gaactgcggg
    23521 tgtatgacac tgatgatgaa gagaatcatc aggagtttac ctgccagtca gatatgactt
    23581 gtgatgataa aggctgccag ctctgcccgc gcggcgtatt acccgttggc gatcgcggca
    23641 caaccctgaa gtggcggctg gctgacactc atacgcagta cggtattttg ctggcgaccc
    23701 tgccgcaggg gcgtcatctt agccatgatc aacaacaact ggtggatacc ctggttgaac
    23761 aactcaccgc cacgctggcg ctggatcgcc atcaggaacg tcagcaacag ttgatcgtga
    23821 tggaagagcg tgccaccatt gcgcgcgaac tgcatgattc tattgcccaa tctctctctt
    23881 gcatgaagat gcaggtgagt tgcttacaga tgcagggcga tgcgctgcca gaaagcagcc
    23941 gcgaactgtt aagtcagatc cgtaacgaac tgaatgcatc ctgggtgcag ttgcgtgaat
    24001 tgctcaccac attccgtttg cagctcaccg agcctggatt acgtccggcg ctggaggcga
    24061 gttgcgaaga gtacagcgcc aaatttggct tcccggtgaa gctggattat caattgccgc
    24121 ctcgtctggt gccttcgcat caggcaatcc acttgttgca aattgcccgt gaggcattaa
    24181 gtaacgccct caaacattcg caagcgagtg aggtcgtggt gacggtggcg caaaacgata
    24241 atcaggtcaa actgaccgtc caggataacg gctgcggcgt gcctgaaaat gccatccgca
    24301 gcaatcacta cggcatgata ataatgcgcg accgtgcgca aagtttacga ggcgattgcc
    24361 gcgtccgccg tcgtgaatca ggtggcaccg aagtggtggt cacctttatt cccgaaaaaa
    24421 ctttcacaga cgtccaagga gatacccatg agtaatcagg aaccggctac tatcctgctg
    24481 atcgacgatc acccgatgtt gcgaactggc gtaaaacagc ttatcagtat ggcaccagat
    24541 atcactgtgg ttggcgaagc gagtaatggc gaacagggta ttgaactggc ggagtctctt
    24601 gatcccgatc tgatcctgtt agatctcaat atgcccggca tgaacggtct ggaaacgctg
    24661 gataaactgc gcgaaaagtc cctctccggg cgcattgtgg tattcagcgt ctctaaccat
    24721 gaagaagatg tggtcaccgc actgaaacgc ggcgcggatg gctatctgtt aaaagatatg
    24781 gaaccggaag atctgctgaa agcgttacat caggcagctg ctggcgaaat ggtattaagc
    24841 gaagcattaa cgcctgttct ggccgccagc ttgcgcgcta accgtgccac tactgagcgc
    24901 gatgttaacc agttaacccc acgcgagcgc gatattctca agctgattgc ccagggtttg
    24961 ccgaacaaga tgattgcccg ccgcctggac atcaccgaaa gcacagtaaa agttcacgtc
    25021 aagcacatgc tgaagaaaat gaagctaaag tctcgcgtgg aagctgcggt atgggtgcat
    25081 caggagcgca ttttctgatt acggttccca gcgcagttcg tcgtttgaca atgcgtcgaa
    25141 cggttcgaca agcgttagcg tgatctcatt ggaggagaca cgctgcccct ggttatcttc
    25201 caccactaca gacaatcgcc agtgattgct cgccccttcc ccgttctgcc agtcaggcat
    25261 gatcagcgtc cagccctcct cactgttggc ttgtgcgccc ggcgtcaaac ttaatatctg
    25321 cgtatcgccc tgccaaatca gttgccgaat accgtaacga ctgcggattt gtaatttcag
    25381 cggcactgtt tcgccaggtt ttagatccca cggcggtgtc gccagaaaca ccgttaacgt
    25441 ttttcgctgt cggtactcaa gagtcggaag attatttcgc tgcggattgt catagcgact
    25501 accacgtaac gactgacttt cgacaacctc gcccgcagaa agttgttttt tgagcggtac
    25561 accaaagcgg tagttaagat tcagcccgag gttattttga ttctcgccac tttcaccctg
    25621 tttatgctgg gccgtcacag tgactaaggg cacaggggtg taatttaatc ccagactcaa
    25681 cgcgacggga ttgtgataac ccgtaccaga gttaaacaaa tcgacacgat caccaaaata
    25741 ctgttctacg ctgacactgg tattgaggtg ttgatagaac ggcatgcgca tccgggctgt
    25801 caggtcgtac ccgcgcgcca tccgttgttc ctgcgtggca gtctgttcat gccatgcagc
    25861 aaacggctga taaaagtttg ccgatagtcg caaatattcg ccccacgctt cagcgccaaa
    25921 gcccgctcgc tgaagatttt cgtccagcaa gttgtcataa aaagtgttat aacccaccag
    25981 ccagctgccg cgcgcccagc gttgcccaac gcccacattg ctcaccagcc catcatcctg
    26041 ctgagtaaga ccaagctggc tccaggtgag ataacgatca ttatcttgta acggcacaaa
    26101 ccagcttcca cgactgccgg tgaaatgtcc ttcgttatcc actttgacgt caacactggc
    26161 atttccccac ggtgatagcc aggactctac gtgctgatta acctgttgac taagcgcatc
    26221 gcggactttt cccaatgcga aagcttttgc ctgctcgcca gtatccagcc cgttatcatt
    26281 catactggtt tcgccgaaat ctttcacaat ttcagcaaag tgtttttccc catcatgatg
    26341 ttcaggtgcc atgcctaaat ccggcagacc atcattgcta ttctcaaagt gatttgccgc
    26401 tttttgctcg aaggtagatt gtgcgttagc tgtaccgcct gccgccagca agagtaaata
    26461 aaacggaata atgcgaggaa cgaaacggct caaaacgtcg gtaacttccg gtaaaaatga
    26521 aaaaccatag ccttaaagaa taacgattac tggcaccttt tgtcgtattt tctcgcgctt
    26581 tcaggaaaat ccttaaaaat gtgaccgatt ggtcagacgc tgcagaagca gtaagataga
    26641 agatgagtgt ctaattccct gaatacagga gtaaataatg caaaaaatcg tgatcgttgc
    26701 caatggcgca ccttacggga gcgaatcctt gtttaacagc ttgcggctgg ccattgcgtt
    26761 acgagagcag gagaacaatc tggatctgcg tctgttcctg atgtctgatg cggtcacagc
    26821 cgggttgcgc gggcaaaaac caggggaagg ctacaacatt cagcaaatgc tggagatcct
    26881 taccgctcag aatgtaccgg tgaaattgtg caaaacctgt accgacgggc gcgggattag
    26941 tacacttcct ctgattgatg gggtggaaat cggtactctt gttgaactgg cgcaatggac
    27001 gctgtcagcc gataaagtgc tcacatttta atgtttgagg gggcgtcgga cgccccacta
    27061 tgagggaagt ttactcaggc gaatcccggc cgtaacacac catccggata attctccgcc
    27121 agcagttttt ttaccgatac cagcaaatca ttcagcccat cgtcctgcat tcccagtttg
    27181 ataagctcct gttccagtga aaacaggtat tgtgcattgg ttcccagcgg accgctcgcc
    27241 gcggcaatca acggcgctat gacctgagcg cgagtgtcag attcatattc tggatgtcgc
    27301 gggtccataa taaacacaat ggcgtttact gtgcgtccat catcaagatc aagctgacac
    27361 caggttggca gataacagcc ggtaatcatc tcacgcttcc acagcagggt tagttcctgc
    27421 tccagcgtct cttctggcag tcgataggcg acgcctgtgg tgcgtccgcc ctctttcagt
    27481 gcaagcattc gtcccggctg gtgcgcagtc ccgcgcccgg cggtcagacg caggcaaaac
    27541 gcgcgatgcc atccaaccag tgtaccggtg cacgactcgg taaactccag tgccggattc
    27601 cacatcaacg atccataacc gaagatccac accggtccct catcaggtcg acaagccagc
    27661 gtcgccgcca gcgaagccgc ccgttgttct gctgaccata agagtgattc ttcaatggca
    27721 ccaaatgccg ttttacaatc ggcattcatc aagaaatcac gcgttatcac cttacacctc
    27781 cgccactgca tacttccctg cgacaacttt ttggtgtctt ccggacattt ttactgctca
    27841 ttttgaaacc actaacggga caatatgcaa tccacggacc acttgcaagg caacaccgca
    27901 cgaatagctt taactaagct accggtttga cgatttttta tgccatttat catcatcccc
    27961 tttggcatat tcatgcttca cagcagccca ggccacttta tgcgctgttt cttcgcgact
    28021 ggcgtcatca cgccgatctt ctttatcttt atattgatcc catgcgctgt tgaacgcttc
    28081 tttatagata tcctgggcat gagacggaag cacgtgcttt acgctttccg gcagatcgct
    28141 tttcgtttta tacgacatcg ttgactcctt ttacaaagtt gaacattaag tgtggtcaac
    28201 aatatctact cttgccaaaa cataagaatt ttcatgtcag actcttctta agaagtgcta
    28261 aaacaaacat tgtctaaacg tattactaat ttttatgccc aaatgagtaa aaaaccgaca
    28321 gcggggtaaa aattcgtaaa atgttaccga tatccagaca atttctgtta tttttcctac
    28381 ctatttttta cattttcgat aaattcatct gcaaagggat aaccataatg tcaaatgctc
    28441 aagaggcggt aaaaacccgc cacaaggaga cttcgcttat tttcccggtt ctggcgctgg
    28501 tagtgctgtt cctgtgggga agcagccaga cactaccagt ggtcattgcc atcaatcttc
    28561 ttgcgcttat tggtatttta agtagcgcct ttagtgttgt ccgtcatgcg gacgtattag
    28621 cccatcgcct gggagaacct tacggttcgc ttattcttag cctttcagtg gttattcttg
    28681 aagtcagttt gatttcagct ttaatggcaa ccggcgatgc cgcgccaacg ctaatgcgtg
    28741 atacgctcta ttcaatcatt atgattgtta caggtgggct ggttggcttt tcattattgt
    28801 tgggcggacg taagtttgcc acccaatata tgaatctgtt tggtatcaag cagtatttaa
    28861 ttgcgctgtt ccccctggcg ataatcgtac tggtatttcc aatggctctg cctgcggcga
    28921 atttttcaac aggtcaggcg ttactggtag cattaatttc tgcggcaatg tatggcgtat
    28981 ttttgctgat ccagaccaaa acgcatcaaa gtttgtttgt ctacgagcac gaagatgaca
    29041 gtgatgatga cgacccgcac cacggtaaac cgtctgccca tagcagcctg tggcatgcca
    29101 tctggttgat tgtccatctg attgccgtta ttgcagtcac caaaatgaac gccagcccgc
    29161 tggagacatt gctcgacagc atgaatgcgc ctgtcgcctt tactggcttc ctggtggctc
    29221 tgttgattct gtcgccggaa ggtttaggtg cgttaaaagc agtgttgaat aaccaggtcc
    29281 agcgcgcgat gaatctgttc tttggttcag tgttggcaac catttcgctt accgtacctg
    29341 tcgtcacgct aattgccttt atgacgggta acgaattgca gtttgcactt ggtgcgccag
    29401 aaatggtggt gatggtggcc tctttagtac tgtgccatat ctccttctcc accggacgaa
    29461 ctaacgtgct caatggcgca gcgcatctgg cactgtttgc cgcctatttg atgacgatat
    29521 ttgcctgaca gttatatctc ctccgatgac ggaaatcggg ggagataata taaattcctg
    29581 cttattatat ttcttcatac tagctggaca ggttgttaat cagaattgca agcctgtacc
    29641 acaacctgca cgaaatgact gcaacagctg ttgaaaaacc ggaatgtact ctttattgca
    29701 aacatttcag ggatgaagtg aagatgaatt gcgacgtagt atccacacca agccggcgtt
    29761 atccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggaaaga
    29821 gaaaaccccc gcacgttgag aggtacaacc tgacaacaga acgggggcta atcttgaacc
    29881 agaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
    29941 cgcgcagttt gccatgattt tctggcacaa cctggcggca ccgatcctgg cggggattat
    30001 taccgcagtg attgtcagct ggtggcgtaa ccggaagtaa ggtgaaaacg ggcgtcaggc
    30061 tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg cggccttttt
    30121 tgtatccggc acccatgaaa aaagtcttaa cgcagaacgc taagacttta tttttcaggc
    30181 aaaaaagatt acttgctggt atccagttct tcgaaacctt tcaccagatc atcaatcgct
    30241 ttcatttgct tgaggaacgg ttccagttta gccagcggca gcgcggatgg accatcacat
    30301 ttcgcatgtt ccggatccgg atgcgcttca ataaacagcc ccgccagacc taccgccata
    30361 ccggctcttg ccagctcagc cacctgagca cggcgaccgc cggaagctgc gccaaatgga
    30421 tcgcgacatt gcagtgcgtg ggtaacgtcg aaaatcaccg gcgaattacc agacactttc
    30481 ttcatgatgc tgaagcccag catatcgaca accaggttgt catagccgaa gttagcaccg
    30541 cgatcgcaaa gaatcacttt ttcgttgccg ccttctttga atttatcaac gatattaccc
    30601 atctgtcccg ggctgacaaa ctgtggtttc ttgacgttaa ttaccgcacc ggttttcgcc
    30661 atggcttcaa ccaggtcagt ctggcgagca agaaacgccg gcaactgaat cacatccacg
    30721 acatcagcaa cgggctgtgc ctgacttggt tcgtgaacgt cggtgataat tttcacgcca
    30781 aaagtctgct tcaactcctg gaagattttc atcccttctt ccaggcccgg tccacgataa
    30841 gagtggatgg aggagcggtt ggctttgtca aaagaggctt tgaacacgta agggataccc
    30901 agtttctggg tcacggttac gtagtgctcg caaatgcgca tcgccagatc gcgagattcc
    30961 agcacgttca taccgccaaa cagtacgaac ggcaggtcat ttgctacgtt gatgtcacca
    31021 atgctaacca ctttttgttt cataggatcg ccttaatctt gagtaaaatg tcgattaatt
    31081 aatgcagcac aatatgttta tgcgcgatgt tatttatttg cgcacggatc atttcgctga
    31141 tcgggtcttc cggacactgt tcaacgaaat aacttaaatc gttcaacgca acgtgttcgc
    31201 aatccagttg cgcataaatc aacccgcgat cgcgaatttc atagggatct tccgggttga
    31261 attgtaataa agcttcgctg gtgcgtaacg ccagctccat ctgattttct tccatcaacg
    31321 aagctttgag tgtatccagc aatttgcgga ttacctcaat gttatcagct tcatcaaggt
    31381 cttcataaaa cagttccgcc gacgggctga tatttccctt taaccacaca tccagcatat
    31441 gttcgcttaa cgattcaccg ttaaaaggat taatcagcca aatttcgcca tccggacatt
    31501 caatgcgcaa tatcagctgc gtagggaaaa tcaccggcag cagcggcaaa tcgagacgat
    31561 tcgcgaccca taataaaacc gcacctaatg ataccgcact gccctgtcga ttctttaaca
    31621 cctggtccag ccataatgca tcggaaagac gataaacgcc gcgtgaggct ttaaatcccc
    31681 agtcgccgta aaacagcgcg atcagttttt ccaactgctc ttctaaaggc agaagctggc
    31741 taatttcttc cttcgccaga ctaacgagac gctccagttc gtcgtaaaca tcttgcgagg
    31801 gaaaatcgcg gcggattgct tcgcaagcca ggatcatgcc ttcgcacaat ggcgctttat
    31861 taaattcgaa atcagctaac gatctcatga cttaccccag taacggtact tttgtggtgg
    31921 cgagtttaat gatgatgtac agcaccacca acgccagcgg gaaggcaata atgcgcgcct
    31981 gctgactgtg catccgacga taatcgagtg caataaaacc caaaacgata taaatgataa
    32041 ctccaaacag cttttcagtc agccactgtg cctgcccgga aaatggctgg atgtgcgctt
    32101 tagccatcaa cgcaatgccg cttaacaata acagcgtatc gacaaccggc ggcacaatgc
    32161 gcgtccagcg agcaaatgcc tgtggatgct tctgataacg tagccagaaa cgtaaggtca
    32221 atagcccaac agaaagcgcg atactaatga gatgaacgct aagcaacgta gaaaaacttg
    32281 tcattgataa tagcggccga gcgttacgcg ctcgttatcc ccatagtcac ggcaggtttc
    32341 gacgtcatga taccccgcga ggataaatgc ctgtcgcacc gcttcaccct gctgccagcc
    32401 atgctccaga agcagaaagc cgccggatac cagcgcgtta cgcgactgtt cgatgatatg
    32461 cacgatgtct gccattccac tgtctgccgc aaccagcgca gtgagcggct caaagcggac
    32521 atcgccttgt tgaagatgtg ggtcctgctc gtcaatatac ggcggattgc tgacaatcat
    32581 cgcaaactgc tgcccggcta gcgcgctaaa ccagtcgctt tgcagaatgt ggatattttt
    32641 gatagccaga tgctgggcat tacgttgtgc cagggcgaca gcatcgggca tacgatctac
    32701 agcgataatt tcgcagtccg ggcgctcgct ggcaagcgca agcgcaatcg ccccggtacc
    32761 cgttccgaga tcgagaatac ggcaaggttg ttcaggcaac cgcgccagtg cctgctccac
    32821 cagacactcc gtatccgggc gcggaattaa ggtcgctggc gaaacaaata acggcaacga
    32881 ccagaattct cgcaccccgg ttaaatgagc aatgggttca ccatcgcgac gacgcgtcag
    32941 tagcgcatca agttgctgac attgttcgtc agtcagctgc gtttcaccaa aggcgagaat
    33001 aaaagtacgc cctttgccgg taacatgctc cagcaggatt tcagcatcac gccgcgggct
    33061 ttcgctcgcc tgaagttggc ttattgcttc acgtaaccag tgttgatatt ccattattcc
    33121 tgctcggaca acgccgccag ttggtcggcc tgatgttcct ggataatcgg ttcaatcagc
    33181 atatccagct taccttccat cacttcatcc aggcggtaga gcgtcaggtt gatgcggtga
    33241 tcggtaacgc gcccctgcgg gaagttgtaa gtacggttac ggtcgctgcg atcgccactc
    33301 cccagcaggt tacgacgggt agacgcttcg gcctgttgcc gttttgccat ttcagcagcg
    33361 tggatgcgag caccgagtac agaaagtgct ttagctttgt ttttatgttg tgaacgttcg
    33421 tcctgacatt caacaacaat cccggtcggc aagtgagtaa tacgaattgc cgaatcggtg
    33481 gtgttaacgt gctgaccacc cgcccctgac gagcggaaag tatctatgcg caaatctgct
    33541 gggttgatgt ccggcagttc tgcatcaggc agttctggca ttaccgcaac ggtacaagca
    33601 gaagtatgaa tacgaccctg cgattccgta gcaggaacac gctgcacgcg atgaccgcca
    33661 gattcgaatt tcaaacgacc atatacacca tcaccgctaa ttttggcgat gatctcttta
    33721 taaccaccat gttcaccctc gctggcgctc atgatttcta cacgccagcg gcgggcttcg
    33781 gcgtaacggc tgtacatacg gaacagatcg ccagcgaaca gcgccgcttc gtcgccgccg
    33841 gttccggcac ggacttcgag gaaggcgtta cgttcgtcat caggatcttt tggtagtaac
    33901 agaacctgta attgctgttc cagttgctcg cttttttctt tagcttcgcg cagttcatcc
    33961 tgcgccatct cacgcatttc aggatcgtca agcatcatct gtgcggtttc gatatcttcc
    34021 tgaacctgtt gccagtcggt aaaacagcgc gaaacatcac ttaactgcgc atattcgcgt
    34081 gataatgcgc gaaaacgttc ctggtcggcg atagtttgcg cgtcacccag caacgcctga
    34141 acttcttcat ggcgttcatg cagggcttcc agtttggcaa cgatagaagg cttcataggc
    34201 gtaaatgcac cctgtaaaaa aagaaaatga tgtactgcta ctccagcccg aggctgtcgc
    34261 gcagaatatt caggcgttcg ttatccccgt cacgggcggc ctgttgaagt gattttgttg
    34321 gcgcatggat caagcggtta gtcagtttcc atgccagatc ctgcataatg gcttgtgcgt
    34381 cgccgccctg ctcaagggcc gctaacgctt tggcggttaa ctcatcgcga acgtgctctg
    34441 cctggctgcg atactcgcga atagtttcgc tggcgctttg tgcacgcagc cacgccataa
    34501 attcgctggt ttcctgagcg acaatagttt ccgcctcaac cgctgcggct ttacgctgcg
    34561 ccaggttgtg cgaaatgatg ctttgcagat cgtccacgct ataaagataa gcattcgcca
    34621 gtttgccaac ttccggctca acatcgcgcg gaacagcaat atccaccaac agcattggtt
    34681 ggttgcgacg gctttttaat gcgcgctcca ccatgccttt cccgataatc ggtaacgggc
    34741 tggcggtgga actgatgatg atatcggctt cgcgcagacg ttcgtcgata tcactcaggg
    34801 caataatttc cgcgccgact tcatctgcca gaatttgggc acgttcgcga gtgcggttgg
    34861 cgataatcat cttctgtact ttgtgttcgc gcagatgacg cgccaccagc tcgatggttt
    34921 cgcccgcacc caccagcagt actgtcaccg tagagagcga ttcaaagatc tgccgcgcca
    34981 gcgtacaagc cgcaaaagcg acagacacag cactggcacc gatatctgtt tcagtgcgaa
    35041 cgcgtttcgc cacagagaaa gatttctgaa acatgcgttc tagttcgctg gccttcatat
    35101 gacctttttg cgaatcggca aacgcttttt taacctgacc gaggatctgc ggctcgccaa
    35161 ggacgagaga atcaagccca ctggcaacgc gcattaaatg actgaccgca tcgttatcct
    35221 gatgccagta gaggctatta cgcagatcgt cttcgttgag attatggtaa tcacacaacc
    35281 agcgaatgag cgcctcttgc aggtcatccc gttcttcaac gctgagataa agttctgtgc
    35341 ggttacacgt cgacagcacc acgccgccct gcaccatcgg ctgcgcgagc agactgtcaa
    35401 gcgcctgatc gagcttatcc ggcgaaaacg atacacgttc tcgcagcgat acaggtgccg
    35461 ttttatggtt gataccgagt gctaaaaggg tcatgtctgc gggaaataat accaacgttg
    35521 atagggttag tctgcttgca tcatacagga tgcgtaagat caataaaaga gagcgccccc
    35581 ttttggagta attgccgaaa gccgttagat tctggcaatt aagacaacgt gaacatagac
    35641 gatggctaac ggtaacgcta gcattaaggg ttataactgc aacgtatctc aaggacttgt
    35701 cattattatg accctgcccg attttcgctt tatccgcctg ctaccgctag ctgccctcgt
    35761 gctcactgcc tgttctgtta ccacacccaa aggcccaggc aaaagcccgg attcgccaca
    35821 gtggcgtcag caccagcaag acgtgcgtaa tcttaatcag tatcagactc gcggtgcatt
    35881 cgcttatatt tccgaccaac aaaaagtgta cgctcgcttc ttctggcaac aaaccggcca
    35941 ggatcgctat cgtctgctgt taactaaccc attgggcagc acggaactgg agctgaatgc
    36001 tcagccgggt aacgtacaat tagtcgacaa taaaggtcag cgttacaccg ccgatgacgc
    36061 cgaagagatg attggtaaac taaccggaat gccgattccg ctcaacagct tgcgccagtg
    36121 gattttaggt ttaccgggtg atgcgaccga ttacaaactg gacgaccagt accgcctgag
    36181 cgaaatcacc tacagccaga atggcaaaaa ctggaaagtt gtttacggtg gttatgacac
    36241 aaaaactcaa cctgcgatgc cagccaatat ggaactcacc gacggtggtc aacgcatcaa
    36301 gttaaaaatg gataactgga tagtgaaata atgcggacac agtggccctc tccggcaaaa
    36361 cttaatctgt ttttatacat taccggtcag cgtgcggatg gttaccacac gctgcaaacg
    36421 ctgtttcagt ttcttgatta cggcgacatc atcagcattg agcttcgtga cgatggggat
    36481 attcgtctgt taacgcccgt tgaaggcgtg gaacatgaag ataacctgat cgttcgcgca
    36541 gcgcggttgt tgatgaaaac tgcggcagac agcgggcgtc tttcgacggg aagcggtgcg
    36601 aatatcagca ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg ttcatccaat
    36661 gccgcgacgg ttctggtggc gttaaatcat ctctggcaat gcggactaag catggatgag
    36721 ctggcggaaa tggggctgac gctgggcgca gatgttcctg tctttgttcg tggtcatgcc
    36781 gcgtttgccg aaggcgttgg tgaaatacta acgccggtgg atccgccaga gaagtggtat
    36841 ctggtggcgc accctggtgt aagtattccg acaccggtga tttttaaaga tcctgaactc
    36901 ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt cagcaatgat
    36961 tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc ctggctgtta
    37021 gaatacgccc cgtcgcgcct gactgggaca ggggcctgtg tctttgctga atttgataca
    37081 gagtctgaag cccgccaggt gctagagcaa gccccggaat ggctcaatgg ctttgtggcg
    37141 aaaggcgtta atctttcccc attgcacaga gccatgcttt aagccgggca agctgagttt
    37201 cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct ctttaataca ccgcctggaa
    37261 agaatcatgc ctggcccgca cagttttcgg cagattcttt ccaccaatgg acgcatgcct
    37321 gaggttcttc tcgtgcctga tatgaagctt tttgctggta acgccacccc ggaactagca
    37381 caacgtattg ccaaccgcct gtacacttca ctcggcgacg ccgctgtagg tcgctttagc
    37441 gacggcgaag tcagtgtaca aattaatgaa aatgtacgcg gtggtgatat tttcatcatc
    37501 cagtccactt gtgcccctac taacgacaac ctgatggaat tagtcgttat ggttgatgcc
    37561 ctgcgtcgtg cttccgcagg tcgtatcacc gctgttatcc cctactttgg ctatgcgcgc
    37621 caggaccgtc gcgtccgttc cgctcgtgta ccaatcactg cgaaagtggt tgcagacttc
    37681 ctctccagcg tcggtgttga ccgtgtgctg acagtggatc tgcacgctga acagattcag
    37741 ggtttcttcg acgttccggt tgataacgta tttggtagcc cgatcctgct ggaagacatg
    37801 ctgcagctga atctggataa cccaattgtg gtttctccgg acatcggcgg cgttgtgcgt
    37861 gcccgcgcta tcgctaagct gctgaacgat accgatatgg caatcatcga caaacgtcgt
    37921 ccgcgtgcga acgtttccca ggtgatgcat atcatcggtg acgttgcagg tcgtgactgc
    37981 gtactggtcg atgatatgat cgacactggc ggtacgctgt gtaaagctgc tgaagctctg
    38041 aaagaacgtg gtgctaaacg tgtatttgcg tacgcgactc acccgatctt ctctggcaac
    38101 gcggcgaaca acctgcgtaa ctctgtaatt gatgaagtcg ttgtctgcga taccattccg
    38161 ctgagcgatg aaatcaaatc actgccgaac gtgcgtactc tgaccctgtc aggtatgctg
    38221 gccgaagcga ttcgtcgtat cagcaacgaa gaatcgatat ctgccatgtt cgaacactaa
    38281 tcgaacccgg ctcaaagacc cgctgcggcg ggtttttttg tctgtaatat ccatttgtat
    38341 gacctatgcc tccttcacct gccatttagt tgacagatga tgcgctcacg gatgaaacat
    38401 tattgtgaac aaaatatttt cctcacatgt gatgcctttc cgcgctctga tcgacgcttg
    38461 ctggaaagaa aaatatactg ccgcacggtt tacccgtgac ctgattgccg ggataaccgt
    38521 cgggattatt gctatcccgc tggcgatggc gttggctatt ggtagtggtg tggcacccca
    38581 gtacggttta tataccgcag ctgttgcggg gattgtcatt gctctgacgg gagggtcacg
    38641 ctttagcgtt tccggtccga ctgcggcatt tgtggtaatt ctctatcccg tttcgcaaca
    38701 gtttggactg gcaggactgc tggttgcgac cttgctgtcg gggatctttt tgattctgat
    38761 ggggctggca cgctttggtc gcctgattga gtatattccg gtttccgtca ccttaggttt
    38821 cacctcgggt atcgggatca ccatcggtac catgcagatt aaagattttc tcggtctgca
    38881 aatggcccat gtcccggaac attatctaca aaaagtcggc gcattattta tggcgctgcc
    38941 gaccattaat gtgggtgatg cagccattgg cattgtgacg cttggcattc tggttttctg
    39001 gccgcgtctg ggcatacgtt tacccggcca ccttccggcc ttgctggctg gttgcgcggt
    39061 gatggggatt gttaacctgc tcggcggaca tgttgctacc atcggttcgc aattccacta
    39121 cgtcctggcc gatggttctc agggtaacgg tattccgcaa ctgctgccgc aactggtgct
    39181 gccgtgggat ctgcctaatt cagaattcac gctaacctgg gattctattc gcacactgct
    39241 gcctgcggca ttctcaatgg caatgctcgg cgcaatcgaa tctctgctct gcgccgtggt
    39301 gctggatggt atgaccggga cgaaacacaa ggcgaacagc gaactggttg gacagggact
    39361 ggggaatatt atcgctccgt tctttggtgg cattaccgct acagctgcca tcgcgcgttc
    39421 tgccgctaac gtccgtgccg gggcaacttc ccctatctcg gcggtgatcc actctattct
    39481 ggttattctt gccctgctgg tactggcacc gctgctctcc tggctgccac tttccgccat
    39541 ggcagccctg ctgttgatgg tggcgtggaa catgagtgaa gcgcacaaag tggtcgactt
    39601 gctgcgtcat gcaccgaaag atgacatcat cgtcatgctg ctgtgcatgt cgctgaccgt
    39661 gctgttcgat atggttattg ccatcagcgt ggggatcgtg ctggcatcgc tgctgtttat
    39721 gcgtcgtatc gcacgtatga ctcgcctggc accggtagtc gtagacgttc cagacgatgt
    39781 cctggttctg cgcgttattg gcccgctgtt ttttgctgcc gctgaaggct tattcacgga
    39841 cctggagtca cgtcttgaag gcaaacggat tgtgattctg aagtgggatg ccgttccggt
    39901 acttgatgct ggtggtcttg atgcgttcca gcgttttgtg aagcgtctgc ccgaaggatg
    39961 tgaactgcgc gtgtgcaacc tggaattcca gccactgcgc actatggctc gcgcaggcat
    40021 tcaaccgatc ccgggacgcc tcgcattctt cccgaatcgt cgcgcggcga tggcggattt
    40081 ataagtacga gattgaccag tcagcgcaaa ctgactggtc agcaaactgc attatttgtt
    40141 agctatgacg gcgattatcg ctacggcggc aacgtttgtc atagtggcga acccaccagt
    40201 aacggtcgca cacctgttca tgcccgccaa cacgggcacc cgccagccag agaatcgcac
    40261 cgacgaaaat acttagcaca gcaccatgtg cgaaatactg gggcatatta aactgtggta
    40321 actggttgag gattgaatac cccacgccga ccaccattac caccagaccc aaccccatga
    40381 gcacgttacc gagtaacgaa gcgtttttgc gtttcatatg tcacctccgg aactttctgg
    40441 gttgtaacag ggaatacccc tcttccttat gtgtaaagta tagacaacac cctgtgggtt
    40501 atgtgcgggt gtgatcacaa ttacaaccct tatttcaaca aaactttaca aataaacgcc
    40561 tgaacacctt gactttctaa tagtccactt aggcaataat tgcattatct gcatttgata
    40621 cgcagttttt tgtcaagcgg ggccgcaacc agtaaactac gcgccagtta tgtacacact
    40681 caggacaaaa aaacgtgacg attaaattga ttgtcggcct ggcgaacccc ggtgctgaat
    40741 acgccgcaac gcgacataat gctggtgcct ggttcgttga cttactggca gagcgattgc
    40801 gcgctccgct gcgcgaagag gctaaattct ttggttatac ttcgcgagtc acccttggag
    40861 gcgaagatgt ccgcctgtta gtcccgacta catttatgaa tctcagcggc aaagccgttg
    40921 cggcgatggc cagttttttc cgcattaatc cggacgaaat tctggtggcc cacgacgaac
    40981 tggatctgcc tcctggcgtc gccaaattta agttgggtag cggtcatggt ggtcacaatg
    41041 gactgaaaga catcatcagt aaattgggta ataaccctaa ctttcaccgt ttacgcatcg
    41101 gaatcggtca tccgggcgat aaaaataaag ttgtcggttt tgtgttaggc aaaccgcctg
    41161 ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt actgaaatgt
    41221 ggtttacaga tggcttgacc aaagcaacga accgattgca cgcctttaaa gcgcaataag
    41281 tcgttgtctg cggcattttt gccgagtgcc gtgtataata ggcaaagtta tttccatttc
    41341 tgcaatctgt tagcaataac aggttgatta ttaagatatt aaggtgattt aaatcatggg
    41401 attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt tcaacgcgct
    41461 gaccaaagcc ggtattgaag cggccaactt tccattctgc accattgagc cgaacactgg
    41521 cgtcgtacca atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa aaccgcagcg
    41581 tacacttccc acgaccatgg aatttgtcga tatcgccggt ctggtaaaag gcgcatcgaa
    41641 aggcgaaggt ctgggtaacc agttcctgac caacatccgt gaaaccgaag cgatcggtca
    41701 cgttgttcgc tgctttgaaa atgacaacat cattcacgtt tccggcaaag ttaacccggc
    41761 ggatgatatt gaagttatca acaccgaact ggcgctggcg gatctcgaca cctgtgaacg
    41821 tgcgattcat cgcgtacaga agaaagccaa aggtggcgat aaagacgcga aagccgagct
    41881 ggcggtactg gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc gcgcgctgga
    41941 tttaagcgct gaagagaaag cggctgttcg ttacctgagc ttcctgacgc taaaaccaac
    42001 aatgtacatc gccaacgtca acgaagacgg ttttgaaaac aacccgtatc ttgaccaggt
    42061 gcgtgaaatc gcagcgaaag aaggttctgt tgtagttccg gtttgtgctg ctgttgaagc
    42121 agacattgcc gaactggacg acgaagagcg tgacgagttt atgcaggagc ttgggctgga
    42181 agagccgggc ctgaaccgtg tgatccgtgc cggttataag ctgctgaacc tgcaaactta
    42241 cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt ccggttggtg caaccgcgcc
    42301 gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg cacaaaccat
    42361 ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc gcgaaagaag caggcaaaat
    42421 gcgtgcagaa ggcaaagatt acatcgttaa agatggcgat gtgatgaact tcctgttcaa
    42481 cgtctaaatt aaatctgctg tctcatgagg tttcactgaa tctcataaga accgtaaaac
    42541 tctataaaat ccacgcaatt gcgtggattt ttcatttcgc aatgtctcaa ttcatctcat
    42601 aacaacgctg tcaaaaatga gtacatggat gagtacaaca ttcttccatc ttcattttaa
    42661 gtgagtacaa taacggtata ataaataaac aaggccccgt tatgctcact gatacgcaat
    42721 gccgcactgc taagccgaaa gaaaaactct atcgacttaa tgatttcaat ggtctctacc
    42781 tcgaagtgaa acccaatggt aaaaaggcat ggcgctatcg gtttaaactc aacggtaaat
    42841 ccagcatgtt tgcgctgggt gagtacccga cggtaaaact tgccgaagct cgcgagaaat
    42901 gcgagcaagc gcgtaagcaa gtcgcagatg gagttagccc aacacaggct cgtcagttag
    42961 ataagatccg caaggtcaac gacgcatcca acacctttga gctaatcgct aaagaatggt
    43021 tgcagatgaa agactgggcc ggaattacca aaacgcggcg actggatatg cttgaacgtg
    43081 tggtttttcc cgccatcggt aagctccctg tcagagagat cactccacac catatactta
    43141 aaattcttca ggaaaccgct aaacgaggag caccaacagt tgccgctgaa gcccgtcgca
    43201 ccatttcatc agtttttgag ttggcagtcg cgacgcttag agctgatagc gatcccgtat
    43261 ggcctgtgcg caaggccctt ccggctaata agacacagca caaacaggca ctaaatcccc
    43321 aacaaatcgg aaagttacta agctgttttg acaatagtcg tggctcgtat caggttaact
    43381 attgcatgtg gcttatgtgg tggacattgg cgcgtcctgc agaagccacc gaagcagaat
    43441 ggacagaatt caatcttgat aacgccctat ggactattcc ggcagcgcga atgaaagccc
    43501 gcagagaaca cgttattccc ctacccttcc aagctgtcaa aatgctcaga acactacagg
    43561 gattaaccgg gcatcggcag caccttttcc cgggcagaga caatccgcta ggtccaatga
    43621 catcgcactc actgcgccag ctacttaaaa gcctcggctg gagtggtaca tatagcccac
    43681 atgcaacccg gaccacagga agtacccgtt taaacgagtt gggctatcga cctgatgcta
    43741 ttgaggccca acttgcgcac gctgatacca acaatgttcg tcgcacatac aaccatgcaa
    43801 cttatcttga tgagcgtaaa gtgatgatgc aggaatgggc tgatatgttg gacaaatggg
    43861 tagcaggatt caagtagaac ttgctgcaca aagtgaacca cgaggataga acttattcct
    43921 caaggttctg gagtgccccc aagcccgtag acaaatctgc cctatagttt gagcatagga
    43981 ggagtctatg ggcacaccac gatttacccc agagtttaag gaagaagctg tccgtcaggt
    44041 cactgaacgg ggctattccg ttgccgatgt ttctgaacgc ctcggcgtat cggctcacag
    44101 cctttataag tgggtccggt cagtcagacc ggataacagc gggcatcagg cgcaggattt
    44161 actggatgcg cggacggaga ttctcaggct taaagcgcag cttaaacgta ctgaggaaga
    44221 acgggatatt ctgaaaaagg cagcgcggta ctttgcaagg gagcccgact gaagtaccgc
    44281 ttcatcaacg atcaccgtga aatctggtcg atcgtaacaa tgtgtcgggt tctgaaggtc
    44341 gcccgtgcgg gattttacgt ctggcttcat cgccctgttt ctgccggaga aaaggataat
    44401 cagcggttgc tggagctcat ccgcgatgct tatgcactga gcggcggagt ctacggttat
    44461 cgcagggttc acggcgatct tcgtgaaatc ggtgaagtat gcagcagaaa ccgtgttgcc
    44521 agaatcatga agcggaacag gatacaggcc gtacatggct ataaggtccc gcgtggcgtc
    44581 aggggaaggc cgtcactgat agctcctaat cgcgtacaga gggaatttac gatggtaaaa
    44641 cccaaccagg tgtgggtgac tgatattacc tacatccgca cctggcaggg ttggctgtat
    44701 ctggcggtgg ttatcgatct ttttgcccgt aatgtggtgg gctggtcgat gaaaccaacg
    44761 ctgtcgcgcg agctggcact ggatgcgctg ctgatggcag tctggcggcg taagcctgca
    44821 gaaaacgtca tagtgcacag cgatcagggc agtcagtatg gcagtgatga ctggcagcgg
    44881 ttctgcaggg ccaacaatct ggcgccgagt atgagccgtc gtggcaactg ctgggataat
    44941 gcggtggcag aatcgttctt cagttcactg aaaaaagagc gcatcagaaa gcggatatat
    45001 aaaacccggg acatggcccg ggcggatatc ttcgattaca ttgaagtgtt ctacaaccgg
    45061 acccggcgcc acagtcatct gggtggcgtc agcccggagg ccttcgaaaa ggcctcaccg
    45121 tgaggacaga aactgtctac cggaatgtgg tcactccact tgagtcgtct tgctgtactc
    45181 ctttggccaa tccatttagc tctccaacgt gatgttttca acggtaacaa aagtaacaat
    45241 ataacatgca gtcatttcaa cgatctcagt gttaccaccc attttgagca atcatatcaa
    45301 agtaacaaaa ggggaatatt gtgtggttca tcgctgaaag caggctaaga tcacctgccg
    45361 gctatgcata tcatgaaagt gctaaatgtc ccgtcttgtt tatcaaccga gataagctgt
    45421 aattgactga gtggctcccc ttaatgcttt ttctcaatca acagatatgg atgggaccag
    45481 tcagtgacct ctttatatga aaaaaaccca tcgtgctcca gtgcgcaaac gcccttatga
    45541 ctggcaacgt aatcgtaaac agcccggctt tccagtccct gtaacatgtg cgaaagcagc
    45601 tttcggcgca tcacatctga cttcctgcgt ttactgtcaa caacgggtaa taaagtgatc
    45661 cactaaccgt caccaccaac ggtttaatat tgatccacct tgtttactca ggattagctt
    45721 caggtataac cccggccttt cgtttctact tcagtcgata gctctctcct tttatctgga
    45781 ctacgtgtga gtgatgtaag atacggtcca gcatcgccga tgtcagggct gcatccctgg
    45841 cgaacgtctg atcccactgc ccgaacggca ggttggagtc caggatcatc gcgctcctct
    45901 cgtaacgttt agcgatgacc tggaagaaca gcttggcttc ttccagactg aacggcagat
    45961 aacctatttc atcgatgata agtagcttcg gggccatgac accgcgattg agtgtcgttt
    46021 tgtaacggcc ctggcgctat gaagtggata gctgtagcag caggtccgct gttgtgaagc
    46081 gaaccttgat gcctgcccgt agcggcgga
//
LOCUS       DAOFSV010000025        45920 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.025, whole genome shotgun sequence.
ACCESSION   DAOFSV010000025 DAOFSV010000000
VERSION     DAOFSV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.025"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(55..1533)
                     /gene="wzxC"
                     /locus_tag="QBH27_001481"
     CDS             complement(55..1533)
                     /gene="wzxC"
                     /locus_tag="QBH27_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDQ7013807.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(1535..2929)
                     /gene="wcaJ"
                     /locus_tag="QBH27_001482"
     CDS             complement(1535..2929)
                     /gene="wcaJ"
                     /locus_tag="QBH27_001482"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDQ7013808.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(2985..4355)
                     /gene="cpsG"
                     /locus_tag="QBH27_001483"
                     /gene_synonym="manB"
     CDS             complement(2985..4355)
                     /gene="cpsG"
                     /locus_tag="QBH27_001483"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDQ7013809.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(4460..5896)
                     /gene="cpsB"
                     /locus_tag="QBH27_001484"
     CDS             complement(4460..5896)
                     /gene="cpsB"
                     /locus_tag="QBH27_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDQ7013810.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(5899..7122)
                     /gene="wcaI"
                     /locus_tag="QBH27_001485"
     CDS             complement(5899..7122)
                     /gene="wcaI"
                     /locus_tag="QBH27_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDQ7013811.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAAMVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(7119..7598)
                     /gene="gmm"
                     /locus_tag="QBH27_001486"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(7119..7598)
                     /gene="gmm"
                     /locus_tag="QBH27_001486"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDQ7013812.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGSDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(7601..8566)
                     /gene="fcl"
                     /locus_tag="QBH27_001487"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(7601..8566)
                     /gene="fcl"
                     /locus_tag="QBH27_001487"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDQ7013813.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(8569..9690)
                     /gene="gmd"
                     /locus_tag="QBH27_001488"
     CDS             complement(8569..9690)
                     /gene="gmd"
                     /locus_tag="QBH27_001488"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDQ7013814.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(9717..10265)
                     /gene="wcaF"
                     /locus_tag="QBH27_001489"
     CDS             complement(9717..10265)
                     /gene="wcaF"
                     /locus_tag="QBH27_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDQ7013815.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGHPAVVIRKRVETE"
     gene            complement(10281..11027)
                     /gene="wcaE"
                     /locus_tag="QBH27_001490"
     CDS             complement(10281..11027)
                     /gene="wcaE"
                     /locus_tag="QBH27_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDQ7013816.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLHFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(11108..12328)
                     /gene="wcaD"
                     /locus_tag="QBH27_001491"
     CDS             complement(11108..12328)
                     /gene="wcaD"
                     /locus_tag="QBH27_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDQ7013817.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNYRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            complement(12303..13520)
                     /gene="wcaC"
                     /locus_tag="QBH27_001492"
     CDS             complement(12303..13520)
                     /gene="wcaC"
                     /locus_tag="QBH27_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDQ7013818.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(13517..14005)
                     /gene="wcaB"
                     /locus_tag="QBH27_001493"
     CDS             complement(13517..14005)
                     /gene="wcaB"
                     /locus_tag="QBH27_001493"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDQ7013819.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(14008..14847)
                     /gene="wcaA"
                     /locus_tag="QBH27_001494"
     CDS             complement(14008..14847)
                     /gene="wcaA"
                     /locus_tag="QBH27_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDQ7013820.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(14976..17138)
                     /gene="wzc"
                     /locus_tag="QBH27_001495"
     CDS             complement(14976..17138)
                     /gene="wzc"
                     /locus_tag="QBH27_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDQ7013821.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLTGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKVLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(17141..17584)
                     /gene="wzb"
                     /locus_tag="QBH27_001496"
     CDS             complement(17141..17584)
                     /gene="wzb"
                     /locus_tag="QBH27_001496"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDQ7013822.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYALLERSAHQWAQALNAEQV"
     gene            complement(17590..18729)
                     /gene="wza"
                     /locus_tag="QBH27_001497"
     CDS             complement(17590..18729)
                     /gene="wza"
                     /locus_tag="QBH27_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDQ7013823.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTLGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            19039..19188
                     /locus_tag="QBH27_001498"
     CDS             19039..19188
                     /locus_tag="QBH27_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013824.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCRSIWFL
                     GVSLD"
     gene            19389..20972
                     /locus_tag="QBH27_001499"
     CDS             19389..20972
                     /locus_tag="QBH27_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ7013825.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(21424..23277)
                     /gene="asmA"
                     /locus_tag="QBH27_001500"
     CDS             complement(21424..23277)
                     /gene="asmA"
                     /locus_tag="QBH27_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDQ7013826.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(23299..23880)
                     /gene="dcd"
                     /locus_tag="QBH27_001501"
     CDS             complement(23299..23880)
                     /gene="dcd"
                     /locus_tag="QBH27_001501"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDQ7013827.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(23972..24613)
                     /gene="udk"
                     /locus_tag="QBH27_001502"
     CDS             complement(23972..24613)
                     /gene="udk"
                     /locus_tag="QBH27_001502"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDQ7013828.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            24931..28248
                     /locus_tag="QBH27_001503"
     CDS             24931..28248
                     /locus_tag="QBH27_001503"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ7013829.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQVAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIELLENSPL
                     SPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(28355..29203)
                     /gene="alkA"
                     /locus_tag="QBH27_001504"
     CDS             complement(28355..29203)
                     /gene="alkA"
                     /locus_tag="QBH27_001504"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDQ7013830.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSVGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            29337..30689
                     /gene="yegD"
                     /locus_tag="QBH27_001505"
     CDS             29337..30689
                     /gene="yegD"
                     /locus_tag="QBH27_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ7013831.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(30702..32642)
                     /gene="yegI"
                     /locus_tag="QBH27_001506"
     CDS             complement(30702..32642)
                     /gene="yegI"
                     /locus_tag="QBH27_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDQ7013832.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVESVAKIYHTS
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVMIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPSGLLRREYI
                     ILIEIALSGLSLLLCGLQTEPRYIILVPVLAAIWIIGSLTSKAYKVEVQRRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRDLAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(32639..33400)
                     /gene="pphC"
                     /locus_tag="QBH27_001507"
     CDS             complement(32639..33400)
                     /gene="pphC"
                     /locus_tag="QBH27_001507"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDQ7013833.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQIQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLDTFTSTERAHKVAAFTDGIQRLALNMLDNSPHMPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(33397..34056)
                     /locus_tag="QBH27_001508"
     CDS             complement(33397..34056)
                     /locus_tag="QBH27_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ7013834.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGSAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(34274..34330)
                     /locus_tag="QBH27_001509"
     CDS             complement(34274..34330)
                     /locus_tag="QBH27_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDQ7013835.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(34602..34658)
                     /locus_tag="QBH27_001510"
     CDS             complement(34602..34658)
                     /locus_tag="QBH27_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDQ7013836.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            34937..36184
                     /gene="mdtA"
                     /locus_tag="QBH27_001511"
     CDS             34937..36184
                     /gene="mdtA"
                     /locus_tag="QBH27_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDQ7013837.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKAASREDTKKGARS"
     gene            36184..39306
                     /gene="mdtB"
                     /locus_tag="QBH27_001512"
     CDS             36184..39306
                     /gene="mdtB"
                     /locus_tag="QBH27_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDQ7013838.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            39307..42384
                     /gene="mdtC"
                     /locus_tag="QBH27_001513"
     CDS             39307..42384
                     /gene="mdtC"
                     /locus_tag="QBH27_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDQ7013839.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRMRFSRKPKQAVTE"
     gene            42385..43800
                     /locus_tag="QBH27_001514"
     CDS             42385..43800
                     /locus_tag="QBH27_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7013840.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            43797..45200
                     /gene="baeS"
                     /locus_tag="QBH27_001515"
     CDS             43797..45200
                     /gene="baeS"
                     /locus_tag="QBH27_001515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDQ7013841.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            45197..45919
                     /gene="baeR"
                     /locus_tag="QBH27_001516"
     CDS             45197..45919
                     /gene="baeR"
                     /locus_tag="QBH27_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDQ7013842.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        10557 a        12484 c        11907 g        10972 t
ORIGIN      
        1 ataaggcgtt ttacgccgca tccggcaacc gttgccggaa ccgaaaacaa ccattcaccc
       61 cgcccgtaaa agcattttca ttttttcgct gcgacaaaac tgacgcttca cttccaccac
      121 cagcggatgg cgcgacagca caatcatcac cacaaacgcc agcacgcccg tggctatttg
      181 caccgccagc agcatcccca gcgccagttg ccctttcagc acaatgccca gcgcataact
      241 gaccaccagc gtcggcagcg agagataaaa cggcagccac aaactcagga tgtactggcg
      301 ataactggaa ccgagcaccg gtttaatcat cacgaaatag ctcagaatgg tgttgataat
      361 ctgcaccagc aggaagccaa gcgtgacgcc gattgcgccc gccatctgcc caccaatgat
      421 gattgccgga ataaacagaa aggttttgaa tacgttgaat ttaaagctga tatcgacccg
      481 cgctttcgcc atcagcagcg aaccaatcgg gttacccacg gagcgcagta gccccacaat
      541 acacagcaat tgcagcaccg gaataatgct gctccacttt tcgccaaata ccagcggtac
      601 aaagttattc gacaccacca ttagccccag cagcgccgga aagttgataa tccccaccac
      661 cgacagcagc ttgtagaagt tgacgcgcag cttttctgta tcgtcctgaa ttttggcgaa
      721 cgccggaaac aacacgcggg tgatgatcgg gttcagcttc atcggtggca caacggccac
      781 gttgtacgcc aggttgtatc ccccagccac gcccgcgccg agaatacgcg ccagcaccag
      841 cgttgaaagg ttggtgttga gatagttgat gatgctgtcc gccgtcagcc aggcaccaaa
      901 gcgtaagttc ggtgccaccg acgccagcga gaaatgcaga ccggggcgat aaattttgcg
      961 gccaaagtag ccaaacagca gcgttctcac cgcactattg accagataac cgagaatcgc
     1021 ggtcatcgcc agcggccaga aatgggcgct aaccaccgta aaagtaaagc ccgccagcac
     1081 cgcgctggtt tcgatcatgc cgattttgtt gaactccagc tccttttgca tcaacgcgcg
     1141 gaactgttgc ccgtggggga tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc
     1201 caggtccggg ttattcagca agtcgccgat gagatcactc aacaaaaaca ccgccacgca
     1261 caccacgatc cccagcccga cgttcagcca gtacagcgtg gtgagttcaa ggtggctgat
     1321 ttcttttcgc tgaataatgg agttagcgat accgaagtca gaaagcgtat ctgccagcgc
     1381 gatgatcacc agcgacacgg taagcaaacc gaactggtgg ttatctataa tccgcgccag
     1441 cacagtcatt tgcaccagac cgaggccgat gatgatcacc gtggcaatcg ccgaccactt
     1501 cgcgccgctg atggtttttt cacgtaagct catatcaata tgccgctttg ttaacgaagc
     1561 ccttgaatac cgtcaggaaa acgattttga tatcgaacca gacgctccat tcgcggatgt
     1621 actcaaggtc gaactcgacg cgtttttcca ttttctccag cgtgtcggtt tcgccgcgcc
     1681 agccgttaat ctgcgcccag ccggtaatgc ccggtttcac tttatggcgc agcatgtagc
     1741 cttcaatgag ctggcgatac tgttcgttat gcgccaccgc gtgcggacgc ggaccgacaa
     1801 tcgacatccc gccagtcagc acattgataa actgcggcaa ttcgtccagc gaggtgcggc
     1861 gcagaaagtt ccccactttg gtgacgcgcg gatcgttctg cgtcgcctgg gtcaccactt
     1921 tgtcgttctc catcactttc atggaacgga acttccacac tttgatcggc ttgccatcca
     1981 tgccgtagcg agtctggcgg aaaataaccg gccccggtga gctcagtttc accgccagcg
     2041 caatgcagca cagcaccggg gagatcagca gcaggataag cgtcgccagc acaatatctt
     2101 ccgcacgttt tatcaggcgg ttaaccccgg aaagcggcgt gtcatacagc ggcaccaccg
     2161 gtacgccgtt catctcttcg atacgggaat ggagaatgtt gaaggtaaag acatcgggga
     2221 tcagcagcac cgaacaggtg gtgtccgcca gttgatggac cagttttttc actcgcgcgc
     2281 cgtcgcacat ctgcatcgcg atatagacgt tatgaatctt gcccgctttc gcatcctcga
     2341 ccagctgttg cagattaccc gcccagtcgt tagaaacgcc acccggtttc gggtcgtggt
     2401 aaacgcccac cacttcaaac cctaaccacg gctgattacg gaagctctcc atcagcattt
     2461 gtccggcggc taaatccccc gccaccgcga ccatgcgctt gttatagcca tgattacgca
     2521 gccagcccgc cccaatacga atacatgaac ggcaaaccac cagtccgatg ctggtcagcc
     2581 cataccacgc cagccagatt ttcaattgcg tgtcgaaatc attgttgaac gccaccagtc
     2641 cggcgctgaa aatcacgctt aacgtccagt tttgtagcaa cagggcaaat tctgtcgctg
     2701 cccgaacacc gcgccatgag cgataaaaat cggtgatgcc gcccagcatc tggaacacca
     2761 ccagcgtaat cagcgccacc aacaggtgca tgtagaggaa tgacagtccg ctcacttcgc
     2821 aaaccagcca tagcccggca aacatgatgg tgatatctga aaagcgttgc accatagaga
     2881 ttaacgatgc attggttttc gctcgctcgc gcttttttag atttgtcatc gttgttcctg
     2941 tttttagccc cttacccgca gtgggggtaa gggaagatcc gacattactc gttcagcaac
     3001 gtcagcagag ttcgcgttcg cgcttccatc agcggcacat caccgcgtga ttccacattc
     3061 aaccgcacca ccggttcggt gttggaggag cgcagattaa agcgccagtc ggcaaaggtc
     3121 atgctgatgc catcggtacg atccaccgcc agtgcctcac ggctaaaatg ctgttccacg
     3181 cggttaatcg cctcaacggg ttgcgccagt ttgctgttga tctcaccgct tgccgggaat
     3241 gccgccatcc ggtcgcgcac cagttcgccc agcgtttttc ctttcagaca caccagttcg
     3301 gcgaccagca gccacgggat catgccgctg tcgcagtaag cgaaatcacg gaagtaatgg
     3361 tgggcgctca tttcgccacc gtagatggcg tcttccttgc gcatacgttc tttaataaag
     3421 gcgtgtccgg ttttcgacat taccggggtg ccgcctgcgg cagtcaccac atcaacggtg
     3481 ttccaggaga gacgtggatc gtggatgatc ttcgcgccgg gatttttttc gaggaacgct
     3541 tctgccagca aaccgacaat gtagtaaccc tcgataaact gccctttttc gtcaaacagg
     3601 aaacagcggt caaaatcgcc atcaaaagca atgcccatat ccgcgccgtg tttaatgacc
     3661 gcattgcggg tgtcgtcgcg acattccggc agtagcgggt taggaatacc gttggggaaa
     3721 ttgccgtccg gcgtgttatg cactttgatt aactccaccg gtgcgccgag ggctttaaag
     3781 cgggcttcaa tggcgtccac caccggaccc gccgcgccgt tcccggagtt aatcaccagc
     3841 ttgagcggcg tgaggttttt gacgttgata taaccgaaca ggtgatcaac gtaagcgtca
     3901 cgcagattga tttgctgata gcgaccgcgt ttggtttcat cgacgggagg aaagtcgttg
     3961 gcttctgcca gacgctggac gtcgcgcagt ccggtatcgc cgctgatcgg gcgagcccct
     4021 tcgcgcacca gcttcatgcc gttgtaatcc atcgggttat ggctggcggt aacttcaatg
     4081 ccgccatcca cgccgagatg gaacgtggcg aaatagattt cttcggtgcc ggacatgccg
     4141 atatccagca cgtcgacgcc cgcatcctgt aaacctttcg ccagcgccag ttttaaggtt
     4201 tcgctggtga ggcggacatc accgcctaac acaatggttt tcggtttgag aaattcgcca
     4261 taggcgcgac caatgcgcca ggcgatatct tcattcagtt cttcgcctaa tttcccgcga
     4321 atatcatagg ctttaaagca ggttaatttt ttcatatcgt tacccttttt caggcaatag
     4381 ttggccctga ccgaaacggc caattttgtt tttgtcgtta tttacatcgc ggtttcgcat
     4441 tcattgcctg atgcgacgtt tacacccgtc cgtagcgatc cgcgaagcgc accacatcat
     4501 cctcttcgag ataagagccg gagcgcactt caattaaatc gagcggaatt ttccccgggt
     4561 tttccaggca atgcgtcgcc cccagcggaa tataaatgga ctcgttttca ccaagcagtt
     4621 tgatatcacc gtcgatagtg acttttgccg ttcccgcgac aaccacccag tgttccgcgc
     4681 ggtgatgatg catctgtacc gacaaccctt cgcccggttt cacggtgatg cgtttcacct
     4741 ggtagcggtc gcccgcgtcg atagagtcat atttgcccca cggacgatac acttcgcgat
     4801 gcacccgatg ctcatggcga ccatcagctt tgatctgctc gaccactttt ttcacatcct
     4861 gcaccgcgtt acggtcagca atcagcactg catctttggt ctgcactacc accaaatctt
     4921 tcaccccgac ggtggtgacc aggccagatt cggcatacac atagctgttt tcagttttgt
     4981 gattaatcac atcgccatgg caaacgttgc cctcggcggt gtgggcactg atctcccata
     5041 aagaggacca ggaaccgaca tcactccagc ccgcatccat cggcaccaca acggcatctg
     5101 ccgtgcgttc catgaccgcg taatctaccg actcttccgg acaggcgaga aacgcctctt
     5161 catccacacg aataaaatcg agatccggat cgacggcgct catcgctttt tcacaggcat
     5221 cgagaatatc cggacgatac tttttcagtt cttcgagata acgtccggca cggaacagga
     5281 acataccgct gttccagtaa tattcgccac ttgccacata ggcctgcgcg gtttccagat
     5341 tcggtttttc gacaaactgc gccacttcaa aggccaccgt atcctgctca cccgccgaca
     5401 cttcaccgcg acgaatatag ccatatccgg tttcaggtag atccggcaca atgccgaagg
     5461 tcaccagctt gcccgcttcg gcatacggca tggcattacg cacggcggca cggaacgcgt
     5521 cttcatcggc aatcacatga tccgccgcca gcaccagcat taatgggtcg ttctccgggc
     5581 tatgacgttt tgccgccagc gccgccagcg caatggcagg tgcggtgtta cgccccgccg
     5641 gttcgagaat aatgttctcg gtgagtttat ttagttgacg cagctgttcc gcgacaataa
     5701 agcggtgctg ctcattgcaa atcaccaccg ggctttcgca ctccacgccg ttcaggcggc
     5761 agatggtggt ttgcagcatg gtgagatcgc ctttcaggca taaaaactgc ttggggtaaa
     5821 gtacgcggga aagcggccat aagcggctac cggagccacc tgccatcaca actggataga
     5881 gtttcgactg cgccataatt atccccgaat atcatttata aattgacgta acacgttctc
     5941 tttatcgagc gtgcgttcgg catattcacg tgccaccgtg ttgtgtttgg gcagcaggag
     6001 cgcctgacga atccccgcca ccagcgcctc gaccgattcc ggttccacgc aaaccgcaat
     6061 gcccggaaag gtttcgcaaa gctgccccag ttcggtgtgg gcttctgcgg taatcaccgc
     6121 gttaccgcct accgccagaa tattggtcag tttcgacggc aatacggcat ctgccgcgcc
     6181 gcgtttttgc accaccagat ggcaatcccc catcttcagc agtgcgggta aagcgtcata
     6241 cgattgcagc ggaaaaaatt gcatgttgcg cagtccacgc tgctgcgcca ttttttccag
     6301 ccgcgctttg ccgccgccct gcccgacaat ggcaaaaata agcggttcat cgcgcaggcg
     6361 atcggcagct tcaataacgt tttccagccc ctgcttttca ccaatattgc cggagtaaag
     6421 aatgattttt ttgttatccg gcaggccaag ctggttacga agggcatcaa catcggcgtc
     6481 tgcgacatgc tgaaaacggg cgatttccga ccagtttggg aagaaaatga cgttttccgc
     6541 cgccacgcct ttttcgatgg ctttattcat catcgaacgc gaaatcgtgg agacgttatc
     6601 gacgttatgc agtccgctac gttcgaacgc cgtcgccagc tgtgccactt tgccgccttt
     6661 gccttttccg gcaaggccca gccccagcat ggcgtccact tcgtaatcct gaatatgcag
     6721 cacggtacgc gcaccagaga gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt
     6781 tggcactacg ccgataatgc gatccggctt ccagcgacgt tgcgccatca gtggaaagaa
     6841 actgctgacg gcaaaactgc cgagatgcaa caggcgtttc agagtgctcg gctgttttgg
     6901 cacgtacagc gggcagcgcc acaccatggc ggccccctct tcccgtttgt accgccaggc
     6961 ggaatagttc tcgcccacct gccactgcgg gtagtaaggc ggcgcggtaa tgacccgcac
     7021 ctcatgacct tgtgccgcca gccattccac catctcgccg gtgtatttgc cgatgccggt
     7081 taactccggc gagtagttaa tgccgtagac cagtattttc ataatccggg tactccggca
     7141 cgcttctcag cgagaaaata ggcgcggctg ttagcgtgaa cattgtcact ggcgagcaac
     7201 gcgtccggcg tcagccagcg gtaatcgtca tgctgctcat ccggcagtaa cagctcttcc
     7261 tccgctactc tgaagcgaaa accgagcacc acatagtgag tggtaaaatc cgagccggag
     7321 aagttatcgt cataaaagtg ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga
     7381 cgcaacccca gttccgccat cgtcagccgc tcaaatgcgg cttccagcgt ttcgtctttc
     7441 tgcacgcgcc ctcccggcac aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca
     7501 agcagaaact cgccgcgact gttctcgaca ataaagtcga gagagacaag cggagtggag
     7561 cgcactaccg tggcaaagtc ttcctgacgt aaaaacatca ttacccccga aagcggtctt
     7621 gattctcaag gaaccactgg taagtgctgg caagccccgc ttccagtgag atttcgtgat
     7681 accagccaag ctgatgcagc cgcgtcacat ccagcagttt gcgcggcgtg ccatccggtt
     7741 tgctggcatc aaaaaccacc cgacctttgt aacccaccac tttggcgatg gtttgcgcca
     7801 gctcacggat agtgcagtca acgcccgtgc cgacgttaat gtgcgacagc atcggctggg
     7861 tgttctcctg ccagacttcg tgtgccagct ccatgacatg aatgctcgcc gctgccatat
     7921 catcaacgtg tagaaattcg cgcatcggcg taccgctgcc ccacaccacc acgtccggcg
     7981 cattctgtgc cgtcgcctca tggaagcgac gcagcaatgc tgggatcaca tgcgaattac
     8041 tcgggtggaa gttgtcgtgt ggcccgtaca ggttggttgg catgactgag cggtaatccc
     8101 gtccgtactg gcgattgtaa gattcacaca gtttgatccc ggcgattttg gcaatggcgt
     8161 aaggctcgtt ggtcggctcc agcgtgccct gcaacaactc gctttctgcc atcggctgtt
     8221 ttgccagttt cgggtagatg caggacgatc cgagaaacag cagtttgttc acgtcgttct
     8281 gatgcgcggc gtgaatgatg ttgctctcaa tcatcatgtt ctggtagatg aaatctgccg
     8341 gatacgtgtt gttggcaaca ataccaccca ctttcgccgc cgccagatag acctggtcaa
     8401 tgctttcgct ggcaaagaaa tcatgcaccg cgcggctgtc cagcaggttc agttcatcgc
     8461 gggtgcgtaa taccagctcc acatcaccgc gctgttcgag ctgccgcctg atggcggaac
     8521 cgaccatccc gcgatggcca gcaataaaaa tgcgttgttt actcatgctt atgactccag
     8581 cgcgatcgcc acgtcgtagc cgtgagattt cagcagagag tgttttttcg ccgcttcaag
     8641 gtcattagcc accatttcag acaccatctc tctgagggtg atttccggtt tccagcccag
     8701 tttttcgtgc gctttggtcg ggtcaccgag cagcgtttca acttctgccg gacggaagta
     8761 gcgcgggtca acggcgataa tcacatcacc cggtttaacg cccggcgcgt catgcccggt
     8821 gacggaaacc acaatgccct tctcttcaac acccgtgcct tcaaagcgca gtttgatgcc
     8881 cagttgtgcc gccgccattt ccacgaactg acgcacggag tactgaacgc cggtggcaat
     8941 aacgaaatct tccggttgtt cctgttgcag catcatccac tgcattttta cgtagtcttt
     9001 ggcgtggccc cagtcacgca gggaatccat attgccgaga tacaggcacg actccagccc
     9061 ctgggcgatg ttggcgattg cgcgggtgat tttgcgggta acgaaagtct cgccacggcg
     9121 cggggattca tggttgaaga gaataccgtt acaggcgtac atgccgtagg attcgcggta
     9181 gttaacggtg atccagtagg cgtacagttt ggcaaccgca tacggagatc gcgggtagaa
     9241 cggcgtggtc tctttctgcg gaatttcctg caccagaccg tacagttcag aagtagaagc
     9301 ctgatagaaa cgggttttct tttccagacc gaggaagcgg atcgcctcga gcaggcgcag
     9361 cgtacccatc gcatcgacgt ctgcggtata ttccggtgac tcaaaagaaa ccgcaacgtg
     9421 gctcattgcc cccaggttgt acacttcatc cggctgcact tcacgcaaaa tgcgcgtcag
     9481 gttggaggta tcactcaggt cgccataatg cagatggaat ttcgggttgc aggtgtgcgg
     9541 atcctgataa atgtgatcca cgcgctcggt gttgaatgac gatgcacgac gcttaatacc
     9601 atgcacctcg taaccttttt ccagcagaaa ctctgccagg taagaaccgt cttgtccggt
     9661 tacaccggtg atgagagcga cttttgacat gtcttattcc tctgtatttt ttgaatttat
     9721 tcagtttcaa cgcgtttgcg tatcaccact gcggggtgcc cccgacaaac cacatttgcc
     9781 ggaagcgatt taaaaacact gcttcgcgca cccacgacgg tgccgtcgcc gatcgtgacg
     9841 ccaggggcaa caaagacatc ggttgccagc cagcatttct cgccaatcac aataggcgtg
     9901 gcgttaatgg tgaaatgttg acttgcatgg tcgtggctac cagtgcataa ataacttttt
     9961 tgcgatatca ccgaatgtgc gccaatggtt atttcaccga gggtatataa attgacctca
    10021 tcgccgaccc acgcgtaatc acctaacgtt aatttccacg gataggtaat ttttactgac
    10081 ggacgaatga ctacgttttt tcctattttt gcgccgaata aacgtaataa aaatgcccgc
    10141 cagcgataca atacttgtgg cgaccaggca aatattgttg cctgtactgc ccaccacaat
    10201 tgcactttaa tagcgttgcc accccggaac cctttcggca ccgagaatcc acttaaatcc
    10261 tgcatcgttt tcccttatat tcagcttttg ttatataagg ctttcgtctt tgaggtcgta
    10321 cgttggcgta aatgccagga taattcagcc caaaagccag gcacgtgcaa tatttgccgt
    10381 tggacttttt tggcgtcagc acacaattcc atattattgg tggtagatac cccacccata
    10441 gaaaattcag acaccaggcc attgagtttt ttaaatgcat aaccggcttt atacattttg
    10501 gccgccagcg cgtaatcgga agaaacttta tactccaggt cataacgcca tttcttcaag
    10561 ccggacaccg ggaaaaatat cgcctgatga ctggcgggca ggctgtggta aatataccaa
    10621 cccggtttgg cgctacgttt aattttatgc ccgtcgccaa aatccagcag cgcatcgccg
    10681 gtgatcatca cgttatcttt ttgcactttt aacttacgga caaaatatgc ggcatcctga
    10741 tgaaaaatat cgcccgagtt gagaaacaac gcgaacttgc cttgcgccat ttcaatacct
    10801 ttgttcatgg cgtcgtagat accgttatct ggttcgctga caaagtgtaa gttatagata
    10861 ccattgagat tttccagata ctcacgggtg ccgtcgttgg aaccgccatc aacaacaatc
    10921 cattcgaagc tgatatcgtc cgcctgcgcc agatgcgcca gcgaggcgtg tgttttgact
    10981 attccttcga ggttacgaaa cgcgacagtg attatgctaa gcaacatgtt ttacctcgta
    11041 atacccgtca tacttcaagc tgcatgtgcg ttggttttcc tcgctcaccc acttcctgca
    11101 actcgaatta tttagggtgt atatttaacg cttttcgcaa aataaacggg cagacgatta
    11161 aaaacgcata ttctgggcta aatatcgaac cggtaaaaaa cagcgatacc ggagtaaaaa
    11221 gatataattg cacgcgaaaa ttgcggttat caccaaaagc gttgatcatc atttttatca
    11281 cttttcccat gtaccacagc gataaaaaca ctgcgaacca ggaaaaataa ataatcagca
    11341 gatacaaacc attgtctatg gtttttccga catccgcacc gttaaatatt ccgaatgatg
    11401 cgacatattc ataaagtgag ccaaatctga ctacaccgtc aatatgggtt aaagaataac
    11461 cgaccatcac cagcggaccg acaatacgat aatacgatga cgatccttct gtacctaaat
    11521 cgcccagacg ggtggaaata taagggaacg cgattaccac accaaccagg aatacagcca
    11581 gagaaatcaa tgctaacggt aactttttct taatcgcctc tttattcaga tattgaaatg
    11641 cccactccag caaataaaaa aggataaagg tcataacccc tgaaaatgaa ccggataata
    11701 ttatccctgc gagaatcata gcatctgttt taggcgtttt gataccaaac tgtttgatgc
    11761 tgagccaaat tgagattaat gccagagcga aaaatgccgg ttcgaaataa agtgctgtcg
    11821 tgcgcttgcc gccgaattta atgaaattca gcacatagct gttgctgtaa atcagatatt
    11881 tcgaaatcga ctccataata ctgctaccgc cggtgaggat aatttgcgcc atctccaccg
    11941 ccgccagcac caccaccagc ccgaccacca gataaaagaa acgtaatatc ttgcgatagt
    12001 tgtgtggcga aatcgtttta aagcgaatac tccacaccat gccaataatg atcacaatat
    12061 agacaaacag catggttgaa gtgacgtatt tactggcatc cagcgactga ccaaacaggt
    12121 agttaaacgc cgtaagtccc gcgccaatcc ccaaggcaat catcaatttt ttaacgctga
    12181 tgcgctctaa aaacagcagt aacaggacag gtaaaaaagt gacgatggtg atggggaaac
    12241 tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta gatcagcggc agcagcaggt
    12301 agctacagat tctgatagaa gttgacatac tcctccagca tctgttgtcc actgtaggcg
    12361 gcgcggctgc gctggctgaa ttcagccagc gtggtaccaa atatcgcctg tgcgatttcc
    12421 ggtttgctta actgcaccag ttgcagcaca tcttcttcgc tgacggtttt accaccggat
    12481 ttttgcagca cttcccgcgc cgcatcgcta tgggtggcga tcaccggcac gccaatcgat
    12541 aacgcctcac acaaaatcag cgggtagtta tcgacgcgag aactgaacac cagcgcatcc
    12601 atctgattga gcacgctcat taacttgcgc ttgtcagttt caaagccgtg attgacaacg
    12661 ttaccagcgg tgaacggcga gaacttacca aaggtatgca gttcgatttt gtcacccagc
    12721 gccatcatct cgcgtaccag ttgctggtta gttttgccgt cgtaacgcag gtcatgcgcc
    12781 accaccgcga ttttcggctt gccctgggtt tcgcgtaccg gaggcaagtc cgccagaatc
    12841 gcttcggttg ccatatcaat gccattattg ataatccggc aacgccctgg accgtacaga
    12901 ctattgaaag catcagccac atgctggctg ggggaaataa actgacagcc cagtgccagc
    12961 atctcgcgga ataactggcg tttacccgcc accagctggt gcgcgcgatc aatcttcacc
    13021 ggtggatagt tatttaaggt cgggcatttc tggcagcccg ttttccagcc ttcgcaaccg
    13081 tcggtaaagg cgcagcgtcc ggtaacgctc cagtggtcgt gtagcgtcca gaccagagtg
    13141 acatccggtt tatggttttt cactttttcg caaaagcgca ccacgctctt aagattcaac
    13201 cagtagctgt gcagcacatg aaaatgcaga accaccgggc ccggtgtgcg agtaatggtg
    13261 cgatataact cattgaaatt gccgaacaga tcgcgattaa gcagacgaaa caacgcaatg
    13321 ttcgccatcg cggtcatccg tggcgtatgt ttgatgacct gcggatagtt ctgatggctg
    13381 acgctctctt tgccgccttt gccgtagccg tagacaaaat gtgacgccag cccctgttgc
    13441 agcgcgcgct ggtggagatc taatgccaca cctgctgccc cgccttccgc cagtcgcaca
    13501 ttaaattgca gaatattcat ttaattacct tcactcgcgc tttttctccc accaccagcg
    13561 cgttgtccgg gacagagtcg agcaccacgc tgcccgcgcc cacggtgacg ttgttaccaa
    13621 tcgtgatatc accaagaata atgacgttgg caccgagttc gacgccgttg ccaatgtgtg
    13681 gacatgccat gttatcggcg ccacgattgc cgatagtgac gccgtgacga atggtgaaat
    13741 catcccccgc gaccacgttt ttattgatca ctacggcgta accgtgatgg atagtaaagc
    13801 gtcggcctat ggtcgcggcg gcctggattt cataaccgaa aaagcattcg gtgataatgc
    13861 gatacagcac cagcagcggg gccgcccaaa gattgttgag gacgttcttt ttgcgccaca
    13921 ccgaacaaaa atgagcggca cgataggcaa gaaccatgca gcacgggcgt aaactccagc
    13981 tgttggcgcg cagatcttcc agcatcttta gcgcccccga ataccatcag ccagacgttt
    14041 gccgttacgc accgacagca gcgtcagcaa agtgcgccag gtcatgcgtt tattgcggat
    14101 ctggtagagg gtaaacagct gatatttttt gctggcgcgg tcgaatttgt ctttgtgctt
    14161 gcggtaaaag tggaagtatc cggagaattt tttcggtgac gaggtgatct gcatttcgcc
    14221 gtgattgata tgcaggatct gcgtcgcctc ttccactttc cacggttcgc cgtactccac
    14281 caccatccgc aggaagatgt cgtaatcctg tgccgctttc agttcggtat cgaacaagca
    14341 ctctttgaaa cgccacgccc aggtaaagac ctgattgcca atgatattgc gtttgtaaaa
    14401 caggcggcgt gaatacggcg atttgggata cagcggcaag cttgccggtt gggaataaac
    14461 ttcgccctgg caaacgtagt cgttagcgta caaaaaggcg tgtgtgacca gttgctgttt
    14521 atgggcgagg aagacgctca ggcggttggg tgtccattca tcatcgtcat cgatcccggt
    14581 gatgtattcc ccttgcgcca gcataatcgc ctggttacgt accgcgcacg ccccgctgtt
    14641 aatgtcgttg tgaatgtaag tgatacgcgg atcgttgagg gcggtgacgt actgttgtag
    14701 ttgttcccag gaggtggagc aatcatccac gatgatcatc tcccagttgt tgtagtcctg
    14761 gcgcaggacc gattttatcg cccgaatcgc cagttgttgg cggttccagg tcggcatata
    14821 gattgaaatc agcggattgt ctttcatagg ttgttctccc ggagtatcga gatgttctgc
    14881 cggtttataa gctggtttac aggttctgtt gccggatgcg gcgtgaacgt aggtcggata
    14941 agatgcgtca gcatcgcatc cgacagcgga tctcgttatt tcgcatccga cttatattcg
    15001 tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga gttcagaatc
    15061 acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc cacttctttc
    15121 aatgtgttga ccgcataacg cgccaccatc aacgtggttc cgacatgacg accaacaatt
    15181 gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc gtagttttta
    15241 ctcgcccact tcaccagttc ggcaaagcgt tcgctcatta acagttcaga aggatttggc
    15301 ggtacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt agcagcggta
    15361 gtaatatcgc cctgtccaat cagaatttcc gacaggccat taacgttatt agtgcccaac
    15421 agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacccg tttattggtc
    15481 tggctaatca ccgccgccag gttggcgcag acaaaggttt taccgattga cgggctaacc
    15541 ccggtcatca tcaacacatt gttctgtgcc tgcatcatcg cgaagtgcag actggtacgc
    15601 agactacgga tggcttcaat cgccagatcg gttggattcc ccaccgccag tagctggctc
    15661 tgtttatagc gtttaatccc tttgatggtt ttgacgctat cgcgcgcttt ctgccactcc
    15721 gacagcggga tgctggcata aacgctgata ccgtgttctt ccagcacctg cgggctttcg
    15781 ataccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat caggccaagg
    15841 ataatcgccc caaggataat cagcactttc ttcggtttca gtacacctgg ctgggtgatt
    15901 gccgggtcga caatgcgcac atcgccgacg gtgctggctt cggtgatttt cagctcctgc
    15961 tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg ggtcagacgc
    16021 acaatttcct gctgggtttt cggcatcgcc gttacgcgac cattaagttt ggctttttcg
    16081 tcttccagcg cctgccgttt ctccagcagc gtgcggtacg ccgggtgaac tttggtgtac
    16141 agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc gatgttcacc
    16201 atcgaatcga gcaccgcttt cgcttccagc ggcagatcaa ctgaatcttt atcctgacgg
    16261 aaggcattca gtttgttttc ggcaacatca aggcggctac gtacttccgg taactgttgc
    16321 gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt ttgttcctga
    16381 tagttacggg cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc accggtgtaa
    16441 gtcaggctca gaacacctgc gtctttgccg ttctccgtta ccgtcaggct gttttgcagt
    16501 tggttgatca tccccagcgt ggagtatttg gtgacggtaa actcactgcc cgggctggcg
    16561 tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc cgcttgccca
    16621 cgggcgctaa agccgccatc gctgctcagg gtgtagtttt tgttgtccag cacattaagc
    16681 gtaaacacct gatccgccat ctccttcggt cggttaaagg tggtcacttt caccgtctcg
    16741 ttctggcgtc ccgtcaggcg ctcccagccc gcaccgaaaa tcgggaacgt gtttttgctc
    16801 accgcaatat caaggtcgag atcgtccacc gttttaccaa gcaccagacg cgaacgaatc
    16861 aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgatcc gatatcctgc
    16921 actaacgaat tgccgctgct ttgttcgatt tgtaccagtg cgtcggcgct gtatatcggc
    16981 gtagcgaaga aggtgtaaac cacggcacag agggcgaata cggcggtaat gccaatcacc
    17041 caccagcgtg cttcaatgat ggtgccaacc aggcgaccaa tatcgatttc atcactgccc
    17101 gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc tctgcgttca
    17161 atgcctgcgc ccactggtgg gcagaccgtt caagtaatgc gtacaccgct gcaaacgttt
    17221 cccggctttt gcgatatgga tcggggattt cacattcgtt atcccagtga ccaaacagca
    17281 tcactttgcc gcgcatctcg ggtgccatct cgcataagcg ttcgatatgg cgcttttcca
    17341 tggtcagaat caggtcgtag ttgcgacaca ggcggcggct gatttgccgt gcacagtgac
    17401 cttccaggga cagttgatgt tctgcggcga cgctgatagc ggtaggatca gcgcccttac
    17461 cgaccaacgc gccgagtcca gcggactcca cttttagctc cgggtgataa cgttgcagta
    17521 agcgttccgc cgtcggggaa cggcaaatat tgccgacaca gacaactaag atgttgttaa
    17581 acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac ggacaccgct
    17641 aatagttggc agcaactgat tgatcagacg gttccagcgg gataccgggg cggtggtgac
    17701 atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc attaccagcg acgtcgcatc
    17761 ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgctct cgcctttcag
    17821 cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatgc cttcagcatt
    17881 gcccagggct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc tctgtttctt
    17941 cacttcaccc atcacaaaca ctttcagatc atcattacgt ggcacgtaaa gaatatcgcc
    18001 ggggtaaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct gcaaagaaat
    18061 gcgctcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg tgtcggtcag
    18121 gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggc acgttggtaa tggcctgttg
    18181 accggattta ttcacctgac cggagatata ggctttttgc gagcggaagg cggcgatatt
    18241 aacgtccacc tgcgggtcag cgatgtacgt cgctaagcgc ccggtaatat cactgcggat
    18301 ttcagcgagc gttttcccca ccacgtgaac tttgccgata tacgggtaga acatggtgcc
    18361 gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccgg ctggcgtggt
    18421 gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga cgcgatactg
    18481 gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg ggcgtgggcg
    18541 taattgatca atcaggcgcg gggtcagcgg ataaacattc accattttgt cgagatcgaa
    18601 atcagcgtcc tgctgtttga tgacgtcttt ccccagcgtc gacatattgc tgcccggaag
    18661 tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt tcattttgga
    18721 tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact attaatagca
    18781 atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg gcatcagtcc
    18841 taaaaaaaat tgatttcatc cgcaggctat tgacagaata attcagactg gtctttcagg
    18901 catccagaca cgctaccgcc cctggctttt tagctaccaa cacactgatt tagcttaatt
    18961 tttcacaccc tctctgcata cagtcgttga tgagaaaggg ttattgcgga aattaacttc
    19021 cgaatataag gtgacattat ggtaattgaa tattggctct ccaataatgc aggaggaagt
    19081 gttacagcta acggaatagc aggcaaggta acaattcggc aattggctat ttttaagaat
    19141 tatattaagt gtcgttcaat atggttttta ggagtttctt tagattgaca atatttaata
    19201 tagtgtctcc acatgcgata tttcttaaat aatgttttat tattagcacc tttaattctg
    19261 ggataatgtg aggttataac cctcaaataa tatgagataa atagtgctgc aacattgcat
    19321 ttttgccccg atttatccat gatcgaattg tgacatttgt catacaacga ataggttttg
    19381 tacttactat ggaatggatt gccgatccgt ctatctgggc tgggttaatc acgctgattg
    19441 tgatcgaact ggtcctcggc attgataacc tggtctttat tgccatcctc gccgaaaaac
    19501 tgccgccgaa gcagcgcgac cgcgcacggg ttaccgggct gctgctggcg atgttaatgc
    19561 gcctgttact gctggcgtca atctcctggc tggtcaccct gactcaaccg ctgttcagct
    19621 tccgctcgtt tacctttagc gcccgcgatt taatcatgct gtttggtggt ttcttcctgc
    19681 tgttcaaagc cacgatggag ctgaacgaac ggctggaagg gaaagacagc aataacccca
    19741 cgcaacgcaa aggtgcgaag ttctgggggg tggtgacaca aattgtggta ctggacgcca
    19801 tcttctcact cgactcggtg attaccgccg tcgggatggt cgaccattta ctggtcatga
    19861 tggccgccgt ggttatcgct atcagcctga tgttgatggc cagcaagccg ttaacgcaat
    19921 ttgttaacag tcacccgacc atcgttattc tctgcctaag cttcctgttg atgattggct
    19981 ttagcctggt ggcagaaggt ttcggcttcg tcattccgaa aggctacctg tacgctgcta
    20041 ttggtttctc ggtgatgatc gaggcgctca atcagttggc tatctttaac cggcgacgtt
    20101 ttctttccgc taatcagacg ctgcgccagc ggactaccga agcggtaatg cgcctgctta
    20161 gtgggcaaaa agaagatgcg gaactggacg ccgaaaccgc gtctatgctg gtggatcatg
    20221 gtaaccagca aatctttaat ccgcaggaac ggcggatgat tgagcgggta cttaatctta
    20281 accagcgtac cgtcagcagc attatgacgt cgcgccacga tattgagcat atcgatctca
    20341 acgcgccgga agacgagatc cgccagttac tggagcgaaa tcagcatacg cggcttgtcg
    20401 ttactgatgg cgatgacgca gaagatttgc tcggtgttgt tcacgttatc gacctgctac
    20461 aacagtcact gcgcggcgaa ccgctcaacc tgcgggtgtt gattcgccag ccgctggtgt
    20521 tcccggaaac cttgccgttg ttacctgccc tggagcagtt ccgtaatgcc cgcacacact
    20581 ttgcttttgt ggtggatgag tttggctcgg tggaagggat tgtgacatta agtgacgtca
    20641 ctgaaaccat tgccggtaac ttaccgaacg aagtggaaga gatcgacgcc cgccatgata
    20701 ttcagaagaa tgcggacggt tcctggacgg cgaatggtca tatgccgctg gaagatctgg
    20761 tgcaatatgt gccgttgccg ctggatgaaa aacgtgaata tcacaccatt gccgggctat
    20821 tgatggaata tttgcaacgt attccaaaac ctggcgaaga agttcaggtg ggggattatt
    20881 tgcttaaaac gttgcaggtg gaaagccatc gcgtgcagaa ggtgcagatt attccgctgc
    20941 gtaaggatgg cgagatggag tacgaggtgt gatggcatcg gccattttac ccaatgctgc
    21001 gtaacgcctt ataacttcag caatgaagcg ttgtctgtgg tacatggaca tcacagcggg
    21061 ggtgtgggtc cggctgtaaa tcgccgaatc gtttcctgta ggccggggca gcccacatgg
    21121 atgtgggctg agggcgcgtt ttacagggat gttacctcgc gcccgtaccc gatagccgaa
    21181 aggaataaga tgagggaacc gcgccagcgg cgatttaccg ccgggagccc gggttgccag
    21241 ggtggtggcg attgagccac cctggcacgt tcaccggctt gagcgttcca tagtagcgag
    21301 gaacatcaag tgaacggaac agccttcgat cttacatatt cagaatataa tggtgccgcc
    21361 acgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc tacaggacag gtcacctggt
    21421 aacctacatc ttctccagca acttcttcac atctttgcca ttgcgggaat ctttattccg
    21481 ctcggcccag tcgttaagac ggcgttttgc ttcatcctgt agatgtttac gcagtaactg
    21541 atccacttgc aggctgtaat taagttgctg ccagttgcca taaacccgca gcggtactgg
    21601 cgtttctttg aggaaatcaa tcagtttgct ttccccgttc cagccaccca cgacccgaat
    21661 atcaaactgg gtgtcgcagg tttgatctgc cagattcaac atgccttccc ctgtcagcgc
    21721 cagcacaggc gattgacctt gcatgtcgtt taacgtcacg acgccatcct tcaacgtcaa
    21781 atcggtggta aagcggtcaa gacgcgtcac gttatcgaag ttttcagcgg ccttcacatc
    21841 accaccatta cgttctaccg cttgctgaat catctgctgg aagttcatcc cttccatgcg
    21901 tgtatcagtc atttcgacat gtgcctgtcc ttgccagttg tggcggaatg cgtcggcatc
    21961 tatgtcagca ccggagaaat caccagccag tgacatcttt ccggtcaacg aaatcggata
    22021 gttaaacgcc ttcagaatgg tgccaatctc aacgttttcc agccgtggct ggaagtttat
    22081 ccgcggattt attgatgtcg cgtccaacgt gcccggcagt gaaacctgtc caccgttaag
    22141 tttgccctgc agttgagtaa tttccagcaa acccgacttg ttggtcattt gcgtggcaac
    22201 atctgtaaaa ttcattccgc gccagcgcac gttactggcc tgcaacaaaa tatcagccgt
    22261 aaagccttgc agtccctgat aggccggttc atcaatacgc gaagaaatga ccgggcgcgg
    22321 caacgttgat tggctctgcc cttgctgcgc ggcaccgttc tcaccgttcg ctgtttcatt
    22381 aagggggatg aggttgtcaa gattcagttg cgggaattgc agtctcagct gccattccgg
    22441 tttctctgtc aacgtgacct gtgcttgccc gctcagcgta ctgtcattgg cggtcaaact
    22501 gatttggtta aatgaaaggc gtttatgtga ctcctgccac tgggcctgga aactcccctg
    22561 cccctgaata ccttgttttg gtaaatcggc cccctgcaac tgccagttaa tttgttcaat
    22621 agccgccgtt aaatcatgcg gataatcaga agcatctact gtaccgttaa gggataatgt
    22681 gagatcgcgc tgatcgcgat taacccgccc ggaaaactca aatgagccac gatgttgggg
    22741 atcttgttcc atttgcaggc ggatattgcg gattgtcacc tgctcgtcat cttcatgctg
    22801 gaacaccaga acgctgtccg ccaccttaag actggatata tcaaacgacc atccgcgatc
    22861 gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac
    22921 cgcttccgtc tgcggcgtca gttggatcac tgcccctttt agcatcacct gcttaacgct
    22981 cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga cgcatgttgt cggcgcgaac
    23041 cagtggctgg cttgcgccct gggcggtgag agacattcgc ccggagagga tactaagctg
    23101 cggccagacg tgccaacgca gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc
    23161 aacttgcttg accatatagt cgcggaaatc attcggattc accagcaaca ctaacgcaga
    23221 taacccggcc accagcacga ccaggagtat catcagcgtc gtcagaaatc gtctcatggt
    23281 atcctcaatg ggcctgaatt agtctttatc gattcggctg gctaccgcgc cctgctggtt
    23341 gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg agagcggctc
    23401 aaagctcagc gcaccaatta acatgcccgg acgcagcgcc agcggcagct taccggagtt
    23461 gtaaaactcc agcacaatgc agccagacca gcccggatcg atacggtgcg cggtgacgtg
    23521 caccatcagc cccagacgcg ccagtgaaga acgcccgtcc agccagccca ccagatcggc
    23581 tggcagcgtc accgactcca gcgtcaccgc cagcgccagc tctcctgggt gaagataaaa
    23641 cgcctcgccc tcgtcgagca cgatttcatc gctcatcacg cggtcaagcg cggcgctcac
    23701 ttcatctttg ggaccgctca gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa
    23761 tttattgccc aggcgcacat ccaccgtcgc gccgttaata cgctccactg gcggacgtgg
    23821 gttgatcgac aaacggcctt catcaagcca ggcttcaata tctcggtcac acagacgcat
    23881 ggcattttct cctttcgagc atcactccct taacgccaat tacgtcaagg gcataccagg
    23941 ttatcactga acggtacaca atttatcaag cttattcaaa gaactgactt attttcgctt
    24001 tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg tccgcatatt
    24061 gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga tattgcgcca
    24121 tcactgaatc cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg cgcatcagac
    24181 agatatccag cggggtatca acgaaaatgg agaagttaag ttcgtcacgt aaacgcgcat
    24241 ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt cggctcaacc gtcaccgttt
    24301 ctttcatacg cgtatgttca acatagctgt aaaccggcag gtcaattgcc gagccgcgtt
    24361 tcaacgcttg taaatgctca agcagcaggc tgtgatccat cgcgctggga tggtcgtagt
    24421 tggtcttaac gcgttcttcc atcgacagat ggctttgatc tttgtaatag cagtcttcgg
    24481 gaattacgcc gatgtgttca tcaccgactt gctcacgtaa ttcacgataa agggtactgg
    24541 caataagact cttgccggaa gccgatgcgc cagcgatacc gataatgacg cactgatgag
    24601 actgatcagt catatattta gcgacctgat taacctggat gttaggaagg gggcgacgaa
    24661 gcgccaaacg cggcaattat agggatttca tccgcctgat accagtcgaa tagcgttgcc
    24721 gcgcgctcag agttaattgt tgacgaagaa ttcccggtgg caaattacgt tgatcagttt
    24781 tatacaaggt aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta cgtcacttta
    24841 tgagcaattc gcatataaaa tgtaaaactt ttgtactagc atgaacacag aaacgaatac
    24901 tgacgacctg gccttgcgga taaagcggta atgagcaaac aatcacagca tgtattaatt
    24961 gccctgcccc acccgctgct tcacctggtc agtttaggtt tagtctcgtt tatcttcacc
    25021 cttttctcac ttgagctttc gcagtttggc acccaactcg ccccactgtg gttcccgacg
    25081 tccatcatga tggtggcgtt ttatcgccat gccgggcgca tgtggccggg aattgcgctg
    25141 agctgctcgc tgggaaatat cgccgcatcc atcctgcttt tttccaccag ctcgctgaac
    25201 atgacctgga cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct gctacgtaaa
    25261 ttgctgccgt ggtataaccc cttgcaaaat ctggctgact ggctgcgtct ggcactcggc
    25321 agcgccatta ttccgcctct gttggggggt gttctggttg tcctgctgac gcccggagac
    25381 gatcctctca gggcattttt gatatgggta ctgtcagaat ccatcggcgc tctggcactg
    25441 gtgccgctgg gattgttatt taaaccacac tatctgctgc gccatcgcaa cccaaggttg
    25501 ctttttgagt cgctgctcac gttagctatc acgctgacgt taagctggct ttcgatgctg
    25561 tatctgccgt ggccttttac tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg
    25621 ccacgaatgg aagccttttt gatcttcctt accacagtga tgatggtatc gctgatgatg
    25681 gccgcggatc cctctctgct tgctacgccg cgtacgtacc tgatgagtca tatgccgtgg
    25741 ctaccgtttt tgctgatcct gctgcccgcc aacatcatga ccatggtgat gtatgccttt
    25801 cgtgcggaac gcaaacacat ttccgaaagc gaaacccgct ttcggaacgc gatggaatat
    25861 tccgccatcg gcatggcatt agtgggcacc gagggacaat ggctgcaatc caacaaagcg
    25921 ctctgccagt ttctcggtta cagtcaggaa gaactgcgcg gactcacctt tcagcaactg
    25981 acctggccgg aggatctcaa taaagatctg caacaggttg aaaagctgat cagcggtgaa
    26041 ataaacacct attcaatgga aaaacgctac tacaaccgca atggcgatgt tgtctgggcg
    26101 ttgcttgccg tctcactggt gcgccacacg gatggcacgc cgctctattt tatcgctcag
    26161 attgaagaca ttaacgagct aaaacgcacc gaacaggtga accagcaact gatggagcgc
    26221 atcacgctgg ctaacgaagc gggcgggatt ggcatctggg agtgggagct aaagccgaat
    26281 atttttagct gggataagcg gatgttcgag ctgtatgaaa ttcctccgca tatcaaaccg
    26341 aactggcagg tgtggtacga gtgcgtgctg ccggaagatc gccagcacgc cgaaaaagtg
    26401 attcgcgatt cattgcaatc acgctctccc tttaaactgg aatttcgcat taccgtgaaa
    26461 gacggcattc gccatatccg cgccctcgct aaccgggtac tgaataaaga aggcgaagtc
    26521 gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa cgaggcattg
    26581 tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc cgtggtctgt
    26641 attgatatgg cgatgaaaat tacctttatg aatccggtgg cggagaagat gagcggctgg
    26701 acgcaggaag aagcgttagg tgttccgctc ctgacggtgt tgcatattac ttttggcgac
    26761 aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa
    26821 gatgtggtgt tgcactgccg aagcggcggc agctacgacg tgcattacag tattacgccg
    26881 ttaagtactc tggacggcag caatattggt tcggttctgg tgattcagga cgtcaccgaa
    26941 tcgcgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc
    27001 gccaatcgag ccagttttga gaagcaacta cgcatcctgc tgcaaacggt aaacagtaca
    27061 catcagcgac atgcactggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc
    27121 gccgggcacg ccgctggcga cgctttactg cgcgaactgg cgtcattgat gctgagtatg
    27181 ctgcgctcca gcgacgtgct ggcgcgactc ggtggcgatg aatttggtct gctgctgcca
    27241 gattgtaatg tcgaaagtgc gcgttttatc gctacacgca ttatcagcgc cgtgaatgac
    27301 tatcacttta tctgggaagg acgtgtacat cgggtaggtg ccagtgcagg gattaccttg
    27361 attgatgaca acaatcatca ggcggctgaa gtgatgtcgc aggctgatat cgcctgttat
    27421 gcctccaaaa atggtggccg gggccgggtg acgatttacg aaccgcagca agttgccgca
    27481 catagcgaac gggctgtgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag
    27541 ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc cgcaagcgcg taatttgtgg
    27601 ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca aacatttcgt
    27661 cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt ccacgatttt
    27721 ttccagcagg ccgcaaaagc gattgccagt aaaggcttaa gcatcgccct ccccctttcc
    27781 gttgccggtt tgagtagcgc cacgctggtg aatgaactaa ttgagctgct ggaaaatagc
    27841 cctctatcac cacggttatt acatctgatt attccggcag acgcgatttt agatcacgca
    27901 gaaagcgtgc aaaaactgcg gctggcggga tgtcggatcg tattcagtca ggtgggccgc
    27961 gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt
    28021 gagttatgcg ccaacgtgca gggaaatttg atggatgaga tgctgattac gatcattcag
    28081 gggcacgctc agcggctcgg gatgaagacc atcgccgggc cagtcgtttt acccttagtg
    28141 atggatacgc tttctggcat cggcgtcgat ctgatttatg gcgatgtgat tgccgatgcc
    28201 caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactaagg gagcgtttgc
    28261 ccattgcctg atgcgacgct aacgcgtctt atcaggccta caaatcgctc attccccagg
    28321 ccggataagc tcgcaccgca tccggcaacc aacgtcatgc ttcgtctggt tgccagcctt
    28381 ccgtatacca gatatgcaac aacgcataag aacgccaggg cttccagcgt tcggcataac
    28441 ggcggatttg tgccggtgtc attcccggaa atcgctgttt aatcagataa tcatccggca
    28501 gaaaaacatc tttcgcctgc cagccacgca aagcaaaata atttgccgtc cagcgcccga
    28561 tacccggaaa ggtttgcagc gttttcatcg cttgttctac gtcgcccggt attgtcattg
    28621 gtaaggtgcc ctccagcgcc gcatttgcca gatgaatcag cgcctctgcc cgtttcaacg
    28681 gcatacctaa cgcttttaat gcctgcgggt cggctactgc cagccgctga ggcgtgggga
    28741 agcagacata atccggaaaa tcatccagcc gttcgccata aagctgtgcc actctggagg
    28801 tcaattttgc cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acgccctgct
    28861 caaaagcgtc aacacaacca ggtaaacgca atccgggccg ccccgcgcct aactttccca
    28921 acgcaccgtt aacaatctgc gggttacatt gcagatcaaa caggcggctc attttcgcca
    28981 gacactcagc ggcaacaggt tctaaaccta cacttaaatt tatgtgcaga gtatggcggg
    29041 ctatatccgg aatagcagtc accacgccgc gatattcgcc taccgccaga ctacgggcat
    29101 aataactgtc cgcgaccgtt tccacgccgc tcaccgcacg ggcggcgaga aatcccaaca
    29161 tccacgacca gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat
    29221 ccgctttcag cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc
    29281 gacggcaaca tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat tatttcgtgt
    29341 ttattggttt tgattacggt acagcaaact gttcagtggc ggtcatgcgc gacggtaaac
    29401 cgcatttgct aaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa
    29461 cgcgtgaagc ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg
    29521 aaacgcaggc gctgttgcgt cgggcgattc gttataaccg cgaagaagat atcgatgtca
    29581 cggcgaaaag cgtgcagttc ggtctttctt cactggcaca atacattgat gatccggaag
    29641 aagtgtggtt tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc
    29701 aggtagcgct gtttgaggat ctggtctgcg caatgatgtt gcacattcgc caacaggcgc
    29761 aggcgcagct gccggaggca attactcagg cggtgattgg tcgcccgatc aacttccagg
    29821 ggctgggcgg tgatgaagca aacacccagg cgcaagggat tctggaacgc gcggcgaagc
    29881 gtgccgggtt taaggatgtg gtattccagt acgagccggt cgcggctggg ctggattacg
    29941 aagccacctt gcaggaagaa aaacgagtgc tggtagtgga tatcggcggt ggtacgactg
    30001 actgttcatt gctgctgatg gggccgcaat ggcgttcgcg tctcgatcgt gaagccagcc
    30061 tcctgggtca tagtggttgc cgtattggtg gtaacgatct ggatatcgca ctggcgttta
    30121 agaacctgat gccgttactg ggcatggggg gcgaaaccga aaaaggcatc gccctgccga
    30181 ttctgccgtg gtggaatgcg gttgccatca acgacgtacc tgcgcaaagt gatttctaca
    30241 gtagtgccaa cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag
    30301 tggccctgtt acagaaagtc tggcgtcagc gtttaagcta tcgcctggta cgtagcgcag
    30361 aagagagcaa aattgcgctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca
    30421 gcgatgaact ggctacgctg attagccagc aagggctgga aagcgccctc aaccagccac
    30481 tggcgcggat tctggaacag gtgcaactgg cgctggataa cgcccaggaa aaaccggatg
    30541 ttatctatct gaccggcggt agcgcccgtt caccgctgat taaaaaagcg ctgacagaac
    30601 agttgccggg cattccgatt gcaggtggcg atgactttgg ctccgtcacc gccgggctgg
    30661 cacgctgggc ggaagtggtg tttcgttaat tgaaaactgg ctcagcaacg gcttaaatca
    30721 gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg gttccatcaa cggcatggtc
    30781 cgtgctggag cttgaaggcg gaatcgctgc aactctgtcg caccgacagt cagcgccgat
    30841 tccagccgat gtcgtttggc ggccattttt gccattaccg cttgtctgtc tgcaggcgtt
    30901 accgcttcgt tcggcctgaa aacaaaacgg cgttcgcaac tcgctttcca gtcgataacc
    30961 gcctgggtca gatgatcacc aaaaccccta acttgcttaa cgctccgtcg ggtaacgtcc
    31021 gctgctgttt caataccaaa ggaccgtaac gccgctttac gcgcagggcc aacgccggga
    31081 atagaggcaa catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgcg
    31141 gtgtcgtgaa gtgctgccag atcgcgcttt tcttcttccg gtaacccaag aatttcgtcc
    31201 ttcatttttt cgagcatcgc ccgtttggcg ataaaacctt ccagcccgcc caactgttgg
    31261 atctggctga ctaaatggtc atagtccatt tttgcgcgat taaaagcctc tcttcgtcgc
    31321 tggacttcta ctttgtacgc tttgcttgtc aggctgccaa taatccagat agccgccagc
    31381 acgggaacca aaatgatata acgcggttct gtctgaaggc cgcaaagcag cagcgataac
    31441 cctgacagtg cgatctcaat cagaatgatg tattcgcgcc ttaacaggcc tgaaggaagc
    31501 ggcctgccag cagcctggaa atgatcgggt aatggcaatt gcaatgctgg cggtgctact
    31561 gacgccatca ccatcgccca gactttcgcc agcacaaaat tcccgccggt ggtaatgacc
    31621 tcttcgccga gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag
    31681 tcagccaaat gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg
    31741 cgtagtaaat caagtgccgc tacccacgct tttgccgtcg gacgcccggt tgccacgcca
    31801 ctttccgtga atgcctgctg aaacatggct tcaacatcgc ccggtaacat cgacagcgga
    31861 atcgatcgtg gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg
    31921 aaatgggcaa tatccgtctc cagcgcatta cccgcatcag agataagcgg tacaccggaa
    31981 taaggatgcc gcccaccaaa caagacgtga aatatcagca acgcaaggcc aaaattatcg
    32041 tgattttctg tgcgttcaaa gccgacaaat gatggcatgg tttgcagctc tggcggcgta
    32101 aaatgcgaca cgccgacttc gcataaatgc agtgtaccat tggcattaat ctgaaaggag
    32161 tcgctatcaa tcatcaccac tttgctgtcg cgacctacca taaagctgtt ctggtttacg
    32221 tcacccacga cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca
    32281 acatagagta gaaaatccca cgcacaatga gggtaactct ggcgacgatg tgccgggcta
    32341 tagatcatat gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt
    32401 ccacctcgtc caccgtgaag cgttgcctgc ggccaggcga cataattcaa caactgcgcg
    32461 tcagctgtcg cagccataaa ggcaagtttg tcctgtttta aggcgggagg cgacgtgtga
    32521 taaatcttgg cgacgctctc gacaaactcc tcgatatcat aaaccgcacc ttcgccgcct
    32581 ttgcccagtt cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc
    32641 atggtaacca cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg
    32701 ggctggacaa aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg
    32761 acgccagtcc attgaaaaac ggggtaaaga aaggcatatg cggagaatta tccagcatat
    32821 ttaacgccag tcgctgaata ccatccgtaa atgctgcaac tttgtgcgca cgctcagtgc
    32881 tggtgaaagt atccagtctg gaaacggcat cttcatcagt aatgaagtgc gtcatattgg
    32941 catattcccc caccatcggc gtgagcggca gttgcaaacc gtgacccaaa tctaccacca
    33001 cgccgccatc gccaatctgc ataatcaagg tgccattagc tgatgaaatc aatcccaaaa
    33061 acgtgcaggc aaaatcgcga accgctaatt ctttggcttc cgcctcggca aaaagtcgct
    33121 gacgaatggt cagcacaatg tccgttgcga ggacatcatt aagccccaat tccccgccct
    33181 gcactttttg cgacatataa gccatcgctt cattgacggc gagcatcgca ccttcgcctc
    33241 cctgcgaggc gctacctgcg ccatcagcga caaataccga caataatggc tgctggtcat
    33301 ttagccaggc aacctgtatc tggcaggcat cctggcaagg caagtccgca ctgatgtgcg
    33361 acgttccaac ggctgatgca tagaccagtc gccagctcac actgacgtcc agccttttgg
    33421 cgcttccagc acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga
    33481 taaccagcta aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct
    33541 gatttgtgcc agcgtcttca tatccgcccc ctgaacgccg atggtaaaga aggcaaactt
    33601 cttatcttct tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggtgc
    33661 accatcggtg atcaggaaaa tccacggacg ataataggaa ataccattgg cacgatattc
    33721 acgtttccgc tcctcaacca tatccagggc tttggtaatg gcagagccca ttggtgtatc
    33781 gccctgggca aacaggatgg gcgggaaaaa attagcggcg ctggtaaagg gttgttccac
    33841 atgcaccggg ccgaacgtca caatgccgag ttccactctt tttaaggcca gcgaatcggc
    33901 aagcagctca tcgcgaaagg taaccaatcc ggcgttaagt tcattaatgg gtctaccgtt
    33961 catagaacca gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc
    34021 aaaatcgctg gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta
    34081 ttgaggtccg gtatcatacc gactgcaata atgaaataag agcaaacgaa gaaactttca
    34141 gaattaggcc cggcggcgca gaagattttg ctattcgcct cgcaagaacg tgggcttaca
    34201 gattgacaac aagggttgag tgacataatt tcaggagtga gggtagagcg gggtttcccc
    34261 cgccctggta gtcttagtat gctgggtagc taatgactaa gagcaccacg atgatgagta
    34321 gcttcatcat gaccctttcc ttatttaagg ccccttcctc gggaggggct ttcccgtttc
    34381 agcgtcccgc tgaaatcttc ggcttacctc ctttcgccct gtatgcagtc tatcgctgac
    34441 ttgcggataa aatagtttca agtgtgatta ttgcgtaacg actcgcaaag aatatttgct
    34501 ggcaggatcg cagactgcaa agtctgcgga ttgacaattg gaccgtgaag gcatactttc
    34561 aggaatgagg gttgagcggg gtttcccccg ccctggtagt cttagtaagc cgggaagctt
    34621 atgactaaga gcaccacgat gatgatccac ttcatcatga ccctttcctt atttatggcc
    34681 ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg aaatcgtcgg cttatctcct
    34741 ttcgccatgc aagcagtcta tcgctaacgc gtagataaaa tagtttcatg tgttattact
    34801 ggatgcgtgc tcgcaaaagt gcccgtcatt cagacgattc ccgacagtgt ttcataattc
    34861 ctccattttt ctcccttatt ggctggctac actagtatca ttccgcgaaa cgtttcagga
    34921 agagaaactc ttaacgatga aaggcagtta taaatcccgt tgggtaatcg taatcgtggt
    34981 ggttatcgcc gccatcgccg cattctggtt ctggcaaggc cgcaatgact cccagagtgc
    35041 agcccctggg gcgacgaaac aagcgcagca atcgccagcg ggtggtcgcc gtggtatgcg
    35101 tgccggccca ttagctcctg ttcaggcggc gaccgcagta gaacaggctg ttccacgtta
    35161 cctcaccggg cttggcacca ttaccgccgc taatactgtt acggtgcgca gccgcgtgga
    35221 cggtcaactg atggcgttac atttccagga aggccagcag gtcaaagccg gcgatttact
    35281 ggcagaaatt gacccaagcc agttcaaagt tgcattagca caagcccagg gccaactggc
    35341 aaaagataaa gccacgctcg ccaacgcccg ccgtgacctg gcgcgttatc aacaactggc
    35401 aaaaaccaat ctcgtatctc gtcaggaact ggatgcccaa caagcgctgg tcagtgaaac
    35461 cgaaggcacc attaaggctg atgaagcaag cgtcgccagc gcgcagttgc aactcgactg
    35521 gagccgtatc accgcaccag ttgatggtcg cgttggtctc aagcaggttg atgttggtaa
    35581 ccaaatctcc agtggcgaca ccaccgggat tgtggtgatc acccaaacac atcctatcga
    35641 tctggtcttt actctgccgg aaagcgatat cgccaccgtt gtgcaggcgc aaaaagccgg
    35701 aaaaccgctg gtggtagaag cctgggatcg caccaactcg aagaaattaa gtgaaggcac
    35761 actgttaagc cttgataacc aaatcgatgc cactaccggt acgattaaag tgaaagcacg
    35821 ctttaataat caggatgatg cgctgtttcc caatcagttt gttaacgcac gcatgttagt
    35881 cgacaccgaa caaaacgccg tggtgatccc aaccgccgcc ctgcaaatgg gcaacgaagg
    35941 ccattttgtc tgggtgctga atagcgaaaa caaggtcagc aaacatctgg tgacaccagg
    36001 cattcaggac agtcagaaag tggtgatccg cgcaggtatt tctgcgggcg accgcgtagt
    36061 gacggacgga attgatcgcc tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag
    36121 cgccactact ccggaagaaa aagccgccag ccgcgaagac acgaaaaaag gagcacgctc
    36181 ctgatgcagg tgttaccccc gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt
    36241 cctgtcgcca ccacgctgct gatggtggcg atcttactcg ccgggattat cggttatcgc
    36301 gccctgcccg tttcggcgct gccggaagtg gactatccaa ccattcaggt ggtcacgctt
    36361 tatccaggtg caagcccgga tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag
    36421 ttcgggcaga tgtctggcct gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt
    36481 atcactttac agttccagct aacattgccg ctggatgtcg ccgagcagga agtgcaggcc
    36541 gcgattaacg ctgcgaccaa cctgctaccg agcgatctgc ctaacccgcc ggtttacagc
    36601 aaagtgaacc cggcagatcc accgatcatg acgctcgctg tcacctcaac cgccatgccg
    36661 atgacgcagg tggaagatat ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc
    36721 ggcgtagggc tggtgacact ttccggcggg cagcgtccgg cggtacgcgt aaaactcaac
    36781 gctcaggcaa ttgccgctct cggcctgacc agcgaaagtg tgcgcaccgc cattactggc
    36841 gcgaacgtta actcagcgaa agggagcctt gacggccctt ctcgcgcggt cacgctgtca
    36901 gcgaatgacc agatgcaatc cgccgaagag tatcgccagc taatcatcgc ctaccagaac
    36961 ggcgcaccaa ttcgtcttgg cgatgtcgca accgtagagc aaggagcaga aaacagctgg
    37021 ctcggcgcgt gggcgaacaa agaacaggcc attgtgatga atgttcagcg ccagcccggt
    37081 gctaacatta tctccaccgc cgacagcatc cggcagatgc taccacagct caccgagagt
    37141 ctgccgaaat cggtgaaggt gacggtactt tctgaccgta ccaccaatat ccgcgcatcg
    37201 gtcgacgata cccagtttga gctgatgatg gctatcgcgc tggtagtcat gattatctac
    37261 ctgtttttgc gcaatattcc ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta
    37321 attggcacct ttgcggtcat ggtgtttctc gatttttcaa tcaataacct gacgttgatg
    37381 gcgttaacta tcgccaccgg attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt
    37441 tcccgctata tcgaaaaagg cgaaaaaccg ctggcagccg cgctcaaagg cgcgggtgaa
    37501 atcggcttta ccattatctc gctgaccttc tcgctgattg cggtattgat ccccctgctg
    37561 tttatgggcg atatcgtcgg gcgactgttc cgcgaatttg ctattaccct ggcagtcgcg
    37621 attttgatct cagcagtggt gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc
    37681 agccaggagt cgctgcgtaa acagaaccgc ttctcccgtg cctcggagaa aatgttcgac
    37741 aggataatcg ccgcctatgg tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc
    37801 ttaagcgtag cgctcagcac gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa
    37861 ggtttcttcc cggtacagga caacggcata attcagggca ctttgcaggc accgcaatcc
    37921 agctcctttg ccaatatggc ccagcggcaa cgccaggtcg cggacgtgat tttgcaggat
    37981 ccggcagtgc aaagtttgac ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaac
    38041 agtgcgcgtt tacagatcaa tctcaaacca ctggatgaac gagatgaccg ggtgcaaaaa
    38101 gtcatcgccc gtctgcaaac ggcggtggat aaagtgccgg gcgtagatct cttcctgcaa
    38161 ccaacgcagg acctgactat tgatactcag gtcagccgca cccagtacca gtttaccttg
    38221 caggccacgt cactggatgc gctcagtacc tgggtgccac agttgatgga aaaactccag
    38281 cagctgccac agctttctga tgtctccagc gactggcagg acaaagggct ggtggcgtat
    38341 gtcaatgtcg acagggacag cgccagccgt ctggggatca gcatggcgga tgtcgataac
    38401 gccctgtaca acgcgtttgg tcagcgactg atttccacta tttatactca ggctaaccag
    38461 tatcgcgtgg tgctggaaca caacaccgaa aataccccag gcctcgcggc gctggatacc
    38521 attcgcctga ccagcagcga cggtggcgtg gtgccgttaa gctcaattgc caaaattgag
    38581 cagcgttttg cgccgctttc catcaaccat ctggatcagt tcccggtaac gactatctcc
    38641 tttaacgtgc cggataacta ttcgctgggc gatgcggtgc aggcgattat ggacacagag
    38701 aagacgctga atctgccggt ggatatcacc acgcagttcc agggcagcac cctcgccttc
    38761 cagtcggcgc tgggcagcac tgtctggctg attgtcgcgg cggtggtggc gatgtatatc
    38821 gtgctcggca ttctgtacga gagctttatt cacccgatca ccattctctc gacgctaccc
    38881 accgcagggg tcggcgcgct gctggcgtta atgattgccg gtagcgaact ggatgtgatt
    38941 gccattatcg gcattatttt gctgatcggt atcgtgaaga agaacgccat catgatgatc
    39001 gacttcgcgc tggctgctga gcgcgagcaa ggcatgtcgc cgcgcgaagc tatttaccag
    39061 gcctgtctgt tgcgttttcg tccgatcctg atgaccactc tggcggctct gcttggtgcg
    39121 ctgccgctga tgttgagtac cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc
    39181 atggtcggcg gtctgattgt cagccaggtg ctgacgctgt ttaccacgcc ggtgatttat
    39241 ctgttgttcg accgtctggc attgtggacc aaaagccgtt ttgcccgtca tgaagaggag
    39301 gcgtaagtga agttttttgc cctcttcatt taccgcccgg tggcgacgat tttactgtcg
    39361 gtcgccatta ccctgtgcgg catactgggt ttccgtatgc tgccagtcgc cccgctgccg
    39421 caggtcgatt ttccggtgat catggtcagc gcctcgctgc ccggtgcgtc accagaaaca
    39481 atggcgtctt ccgttgccac gccgctggag cgctcacttg ggcgcattgc cggagtcagc
    39541 gaaatgacct ccagcagttc gctcggcagc acgcgcatta ttttgcagtt tgattttgac
    39601 cgggatatca acggcgcagc gcgcgatgtg caggcggcga tcaacgccgc gcaaagtctg
    39661 ctacccagcg ggatgcccag ccgtccgacc tatcgcaaag cgaacccatc ggatgcgcca
    39721 attatgatcc tcacgctgac atccgatact tattcgcagg gtgaactgta cgatttcgcc
    39781 tcgacacagc tggctccgac gatttcgcaa atcgacggtg tcggtgatgt cgatgttggc
    39841 ggcagctcgc tgcccgccgt acgcgtcggg ctgaatccgc aggcgctgtt taatcagggc
    39901 gtctcgctgg acgacgtacg caccgccatc agcaatgcca acgtgcgtaa accgcagggg
    39961 gcgctggaag atggtactca ccgctggcag atccagacta acgatgaact gaaaaccgcc
    40021 gctgaatatc agccgctgat tattcattac aacaacggcg gagcggttcg tctgggcgat
    40081 gtggcgacgg tgaccgactc agtacaggat gtgcgcaacg ccgggatgac caacgccaaa
    40141 ccggctattt tgctgatgat ccgcaagctg ccggaagcca atattatcca gacggtagac
    40201 agcatccgcg cccgtctgcc ggaattacaa tccaccatcc cggcagcaat tgatctgcaa
    40261 attgcccaag atcgctcccc tactatccgc gcgtcgctgg aagaggttga gcagacgctg
    40321 gtaatctcag ttgcactggt gattctggtg gtgtttttgt tcctgcgttc gggtcgcgcc
    40381 accattattc ccgccgttgc ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac
    40441 ctgtgtggtt tcagtctcaa taatctttcg ttaatggcgc tcactatcgc cactggcttt
    40501 gtggtggatg acgccatcgt ggtgctggaa aacattgccc gtcatctgga agcggggatg
    40561 aaaccgctac aggccgcgct acaaggtact cgcgaagtcg gctttacggt gctgtcgatg
    40621 agtctgtcac tggtggcggt gttcctgccg ctgctgttga tgggcggatt gccgggtcgc
    40681 ctgttacgcg agtttgccgt gacgctttct gtcgccattg gtatttcgct gctggtttcc
    40741 ctgacgttaa cgccgatgat gtgcggctgg atgctaaaag ccagcaagcc gcgcgagcaa
    40801 aagcgactgc gtggctttgg tcgcatgttg gtagccctgc aacaaggcta tggcaagtcg
    40861 ctgaaatggg tgctcaatca tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg
    40921 ctgaatatct ggctgtatat ctcgatcccg aaaaccttct tcccggagca ggacaccggc
    40981 gtactgatgg gtgggataca ggctgaccag agtatttcgt ttcaggcgat gcgcggtaag
    41041 ttgcaggact tcatgaaaat tatccgtgac gatccggcgg tggataatgt caccggcttt
    41101 accggcggtt cacgggtgaa cagtggaatg atgtttatca cccttaaacc acgcggcgag
    41161 cggagcgaaa cggcccaaca gattatcgat cgcttgcgta aaaaactggc taaagaaccg
    41221 ggagcgaatc tgttcctgat ggcggtacag gatattcgcg ttggcggacg tcaggctaac
    41281 gccagttacc agtacacgtt gttatccgac gacctggcgg cactgcgcga atgggagccg
    41341 aaaatccgca aaaaactggc gacattgcca gagctggctg atgttaactc cgatcaggaa
    41401 gataatggcg cggagatgaa ccttatttac gatcgcgaca ccatggcgcg attgggaatt
    41461 gacgtgcaag ccgccaacag cctgttaaat aacgccttcg gtcagcggca aatctcgacc
    41521 atttaccagc cgatgaacca gtacaaagtg gtgatggaag tggatccgcg ctatacccag
    41581 gacatcagtg cactggaaaa aatgttcgtt atcaataacg aaggaaaagc gatcccgctg
    41641 tcgtatttcg ctaaatggca accggcgaac gctccactgt cggtgaatca tcagggatta
    41701 tcggcggcct cgaccatttc gttcaacctg ccgaccggga aatcgctctc ggacgccagt
    41761 gcggcgatcg atcgcgcaat gacccagctt ggtgtgcctt cgacggtgcg cggcagtttt
    41821 gccggaacag cgcaggtgtt ccaggagacg atgaactcac aggtgatcct gattatcgcc
    41881 gccatcgcca cggtatatat cgtactgggt atcctttacg agagttatgt gcatccactg
    41941 acgattctct ccaccctacc ttcggcgggc gtcggagcgc tgttggcgct ggagctgttc
    42001 aatgccccgt tcagcctaat tgcgctgata gggatcatgt tattaatcgg catcgtgaag
    42061 aaaaacgcca ttatgatggt cgattttgcg cttgaagccc aacggcacgg taacctgacg
    42121 ccgcaggaag ctattttcca ggcctgtctg ctgcgttttc gcccgattat gatgactacc
    42181 ctggcggcgc tgtttggtgc gctgccgctg gtattgtcgg gcggtgacgg ctcggagttg
    42241 cggcaaccat tggggatcac cattgtcggc ggtctggtca tgagccagct tctgacgctg
    42301 tataccacgc cggtggtgta tctctttttc gaccgtctgc ggatgcgttt ttcgcgtaaa
    42361 cctaaacaag cggtaaccga gtaaatgaca gatcttcccg acagcacccg ctggcaattg
    42421 tggattgtgg ctttcggctt ctttatgcag tcgctggaca ccaccatcgt taacaccgcg
    42481 cttccctcaa tggcgcaaag cctcggggaa agtccgttgc atatgcacat ggtcattgtc
    42541 tcttatgtac tgaccgtggc ggtgatgctg cccgccagcg gctggctggc ggacaaagtc
    42601 ggcgtgcgca atattttctt taccgccatc gtgctgttta ctctcggttc actgttttgc
    42661 gcgctttccg gcacgctgaa cgaactgttg ctggcacgcg cgttacaggg cgttggcggc
    42721 gcgatgatgg tgccggtcgg caggttgacg gtgatgaaaa tcgtaccgcg cgagcaatat
    42781 atggcggcga tgacctttgt cacgctgccc ggtcaggtcg ggccgctgct tggcccggcg
    42841 ctcggcggtc tgctggtgga gtacgcatcg tggcactgga tctttttgat caacattccg
    42901 gtgggaatta ttggcgcgat cgccacactg atgttaatgc cgaactacac catgcagacg
    42961 cggcgctttg atctctccgg ctttctgttg ctggcggttg gcatggcggt attaaccctg
    43021 gcgctggacg gcagtaaagg tacaggttta tcgccgctgg cgattgctgg cctggtcgca
    43081 gttggcgtgg tagcactggt gctttatctg ctgcacgccc aaaataacaa ccgcgccctg
    43141 ttcagtctga aactgttccg cactcgtacc ttttcgctgg gcctggcggg gagctttgcc
    43201 ggacgtattg gcagtggcat gttgcccttt atgacaccgg ttttcctgca aattggcctc
    43261 ggtttctcgc cgtttcatgc cggactgatg atgatcccga tggtactcgg cagcatggga
    43321 atgaagcgaa ttgtggtaca ggtagtgaat cgctttggtt atcgtcgggt actggtggcg
    43381 accacgctgg gcctgtcgct ggtcaccctg ttgtttatga ctaccgctct gctgggctgg
    43441 tactacgttt tgccgttcgt cctgttttta caaggaatgg tcaactcgac acgtttctcc
    43501 tccatgaaca ccctgacgct gaaagatctc ccggacaacc tggcgagcag cggcaacagt
    43561 ctgctgtcga tgattatgca attgtcgatg agtattggcg tcaccatcgc cgggctgttg
    43621 ctgggacttt ttggttcgca gcatgtcagc gtcgacagcg gcaccacaca aaccgtcttt
    43681 atgtacacct ggcttagcat ggcgtctatc atcgcccttc cggcgttcat ctttgccaga
    43741 gtgccgaacg atacgcatca aaatgtggct atttcacggc gaaaaaggag cgcgcaatga
    43801 agttctggcg acccggtatt accggcaaac tgtttctggc gattttcgcc acctgcattg
    43861 tcttattgat cacgatgcac tgggcggtac gtatcagttt tgagcgcggc tttatcgatt
    43921 acatcaagca tggtaatgaa caacggctgc aaatgctcgg cgatgcgctt ggtgagcagt
    43981 acgcccagca cgggaactgg cgcttcctgc gtaataacga tcgctttgta tttcagatcc
    44041 tacgttcact ggagcatgat aacaacgaag ataagcccgg ccccggtatg ccgccacacg
    44101 gctggcgcac acaattttgg gtggttgatc aaaacaacaa agtgctggtt ggcccgcgag
    44161 caccagttcc acccgacggc acacggcggc ccattatggt caatggtgcg gaagttggcg
    44221 cggtgatcgc ctcccctgtt gaacgactga cccgtaatac tgatatcaat tttgacagac
    44281 aacagcggca aaccagttgg ctgattgtgg ctttatctac cttattagcg gcgctggcga
    44341 cattcccact ggcgcgcggt ttgctggctc cggtaaaacg actggtggat ggcacgcaca
    44401 aactggcagc gggtgatttc actactcgcg tagcccccac cagcgaagat gagttgggca
    44461 gactggcaga ggatttcaat caactcgcca gcacactgga gaaaaaccag cagatgcgcc
    44521 gcgattttat ggccgatatt tctcatgaac tgcgcacgcc tttagcagta ctgcgcggcg
    44581 aactggaagc cattcaggat ggtgtgcgta aattcacgcc ggagacgctg gcttctttac
    44641 aggcagaagt aggtacactg acaaaactgg ttgacgatct ccatcaattg tctatgtctg
    44701 atgaaggcgc tctcgcctat caaaaagcac cgatagattt gatcccactg ctggaagtgg
    44761 cgggaggcgc atttcgcgaa cgattcgcca gccgtggcct gaaattgcaa ttttccctgc
    44821 cagacagtat taccgtattt ggcgatcgcg atcgtttaat gcagttgttc aataacttac
    44881 tggaaaacag cctgcgctac actgacagcg gcggcagcct gaaaatctct gccgagcagc
    44941 acgacaaaac ggtgcgcctg acctttgccg acagtgcgcc gggtgtcagt gacgatcagc
    45001 tacaaaaatt gtttgaacgt ttttatcgca ccgaaggttc ccgcaaccgc gccagcggtg
    45061 gttccgggct ggggctggcg atttgcctga acattgttga agcacataac ggccgcatta
    45121 ttgccgccca ttcgcctttt ggcgggctta gcattacagt agagttaccg ctggaacggg
    45181 atttacagag agaagtatga ccgagttacc aatcgacgaa aacacaccgc gtattttgat
    45241 cgtggaagat gaaccgaagc tggggcagtt gctcattgat tatctgcgtg ctgcgagcta
    45301 tgcgccaacg ttgattagcc acggcgatca ggtactgcca tatgtgcgcc agacaccacc
    45361 ggatctgatc ctgttggatc tgatgctccc tggcaccgat ggcctgacgc tgtgccggga
    45421 aattcgtcgt ttttctgaca ttccgatcgt gatggtgacg gcaaaaatcg aagagatcga
    45481 ccgcctgctg gggttggaga ttggcgcaga tgactatatc tgcaagccgt acagcccacg
    45541 ggaagtggta gcgcgcgtca aaaccatttt gcgccgttgc aaaccgcagc gtgagttgca
    45601 gcaacaggat gctgaaagcc cgttgattat cgacgaaggt cgttttcagg cttcctggcg
    45661 cggtaaaatg cttgacctga cgcctgcaga atttcgactg ctgaaaacgc tctctcatga
    45721 accaggaaaa gtgttctccc gcgagcagtt gctgaaccat ctttatgacg actaccgcgt
    45781 ggtaaccgac cgtaccatcg acagccacat taaaaacctg cgccgcaagc tggaatctct
    45841 cgacgccgaa cagtcattta tccgcgccgt ttacggcgtc ggttaccgct gggaagccga
    45901 cgcctgccgc atcgtttaac
//
LOCUS       DAOFSV010000026        45446 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.026, whole genome shotgun sequence.
ACCESSION   DAOFSV010000026 DAOFSV010000000
VERSION     DAOFSV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.026"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(47..292)
                     /gene="zapB"
                     /locus_tag="QBH27_001517"
     CDS             complement(47..292)
                     /gene="zapB"
                     /locus_tag="QBH27_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDQ7013843.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            717..1562
                     /gene="glpF"
                     /locus_tag="QBH27_001518"
     CDS             717..1562
                     /gene="glpF"
                     /locus_tag="QBH27_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDQ7013844.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            1585..3093
                     /gene="glpK"
                     /locus_tag="QBH27_001519"
     CDS             1585..3093
                     /gene="glpK"
                     /locus_tag="QBH27_001519"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDQ7013845.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            3264..4274
                     /gene="glpX"
                     /locus_tag="QBH27_001520"
     CDS             3264..4274
                     /gene="glpX"
                     /locus_tag="QBH27_001520"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDQ7013846.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            4371..5117
                     /gene="fpr"
                     /locus_tag="QBH27_001521"
     CDS             4371..5117
                     /gene="fpr"
                     /locus_tag="QBH27_001521"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDQ7013847.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(5122..5550)
                     /gene="uspD"
                     /locus_tag="QBH27_001522"
     CDS             complement(5122..5550)
                     /gene="uspD"
                     /locus_tag="QBH27_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDQ7013848.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(5577..5876)
                     /locus_tag="QBH27_001523"
     CDS             complement(5577..5876)
                     /locus_tag="QBH27_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDQ7013849.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(6088..6528)
                     /locus_tag="QBH27_001524"
     CDS             complement(6088..6528)
                     /locus_tag="QBH27_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ7013850.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            6629..7228
                     /locus_tag="QBH27_001525"
     CDS             6629..7228
                     /locus_tag="QBH27_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDQ7013851.1"
                     /translation="MKPGCTLFILLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            7336..8103
                     /gene="tpiA"
                     /locus_tag="QBH27_001526"
     CDS             7336..8103
                     /gene="tpiA"
                     /locus_tag="QBH27_001526"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDQ7013852.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(8158..8913)
                     /gene="cdh"
                     /locus_tag="QBH27_001527"
     CDS             complement(8158..8913)
                     /gene="cdh"
                     /locus_tag="QBH27_001527"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDQ7013853.1"
                     /translation="MKKAGLLFLVMIVIAVVTAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(9020..10009)
                     /gene="sbp"
                     /locus_tag="QBH27_001528"
     CDS             complement(9020..10009)
                     /gene="sbp"
                     /locus_tag="QBH27_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDQ7013854.1"
                     /translation="MKKWGVGLTFLLVATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(10329..11291)
                     /gene="pfkA"
                     /locus_tag="QBH27_001529"
     CDS             complement(10329..11291)
                     /gene="pfkA"
                     /locus_tag="QBH27_001529"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDQ7013855.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(11472..12374)
                     /gene="fieF"
                     /locus_tag="QBH27_001530"
     CDS             complement(11472..12374)
                     /gene="fieF"
                     /locus_tag="QBH27_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDQ7013856.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(12523..13023)
                     /gene="cpxP"
                     /locus_tag="QBH27_001531"
     CDS             complement(12523..13023)
                     /gene="cpxP"
                     /locus_tag="QBH27_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDQ7013857.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            13173..13871
                     /gene="cpxR"
                     /locus_tag="QBH27_001532"
     CDS             13173..13871
                     /gene="cpxR"
                     /locus_tag="QBH27_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDQ7013858.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            13868..15241
                     /gene="cpxA"
                     /locus_tag="QBH27_001533"
     CDS             13868..15241
                     /gene="cpxA"
                     /locus_tag="QBH27_001533"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDQ7013859.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(15412..16086)
                     /gene="yiiM"
                     /locus_tag="QBH27_001534"
     CDS             complement(15412..16086)
                     /gene="yiiM"
                     /locus_tag="QBH27_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDQ7013860.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSVAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(16235..17218)
                     /gene="kdgT"
                     /locus_tag="QBH27_001535"
     CDS             complement(16235..17218)
                     /gene="kdgT"
                     /locus_tag="QBH27_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDQ7013861.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(17478..18098)
                     /gene="sodA"
                     /locus_tag="QBH27_001536"
     CDS             complement(17478..18098)
                     /gene="sodA"
                     /locus_tag="QBH27_001536"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDQ7013862.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQAYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            18383..19417
                     /gene="rhaT"
                     /locus_tag="QBH27_001537"
     CDS             18383..19417
                     /gene="rhaT"
                     /locus_tag="QBH27_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDQ7013863.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(19414..20280)
                     /gene="rhaR"
                     /locus_tag="QBH27_001538"
     CDS             complement(19414..20280)
                     /gene="rhaR"
                     /locus_tag="QBH27_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDQ7013864.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(20336..21172)
                     /gene="rhaS"
                     /locus_tag="QBH27_001539"
     CDS             complement(20336..21172)
                     /gene="rhaS"
                     /locus_tag="QBH27_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDQ7013865.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHCVLQQVRQLVAQLEQQEEGNDLPSTASREILFMQLLLLLRKS
                     SLQESVENSASRLNLLLAWLEDHFADEVNWDAVAAQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            21460..22929
                     /gene="rhaB"
                     /locus_tag="QBH27_001540"
     CDS             21460..22929
                     /gene="rhaB"
                     /locus_tag="QBH27_001540"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDQ7013866.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            22926..24185
                     /gene="rhaA"
                     /locus_tag="QBH27_001541"
     CDS             22926..24185
                     /gene="rhaA"
                     /locus_tag="QBH27_001541"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDQ7013867.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLLDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKKILSRRG"
     gene            24452..25276
                     /gene="rhaD"
                     /locus_tag="QBH27_001542"
     CDS             24452..25276
                     /gene="rhaD"
                     /locus_tag="QBH27_001542"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDQ7013868.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            25286..25600
                     /gene="rhaM"
                     /locus_tag="QBH27_001543"
     CDS             25286..25600
                     /gene="rhaM"
                     /locus_tag="QBH27_001543"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDQ7013869.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(25641..27035)
                     /locus_tag="QBH27_001544"
     CDS             complement(25641..27035)
                     /locus_tag="QBH27_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDQ7013870.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
     gene            complement(27389..27949)
                     /locus_tag="QBH27_001545"
                     /pseudo
     CDS             complement(27389..27949)
                     /locus_tag="QBH27_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(28031..28864)
                     /gene="fdhD"
                     /locus_tag="QBH27_001546"
     CDS             complement(28031..28864)
                     /gene="fdhD"
                     /locus_tag="QBH27_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDQ7013871.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            29058..32108
                     /gene="fdnG"
                     /locus_tag="QBH27_001547"
     CDS             29058..32108
                     /gene="fdnG"
                     /locus_tag="QBH27_001547"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:29643..29645,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDQ7013872.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            32121..33023
                     /gene="fdoH"
                     /locus_tag="QBH27_001548"
     CDS             32121..33023
                     /gene="fdoH"
                     /locus_tag="QBH27_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDQ7013873.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            33020..33655
                     /gene="fdoI"
                     /locus_tag="QBH27_001549"
     CDS             33020..33655
                     /gene="fdoI"
                     /locus_tag="QBH27_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDQ7013874.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            33652..34581
                     /gene="fdhE"
                     /locus_tag="QBH27_001550"
     CDS             33652..34581
                     /gene="fdhE"
                     /locus_tag="QBH27_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDQ7013875.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AEHNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(34763..35005)
                     /locus_tag="QBH27_001551"
     CDS             complement(34763..35005)
                     /locus_tag="QBH27_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013876.1"
                     /translation="MNPLAGMDMSRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLVNQSQTARTSVPGIWQGCEEDGVEYLRKLREKW"
     gene            35295..36143
                     /locus_tag="QBH27_001552"
     CDS             35295..36143
                     /locus_tag="QBH27_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013877.1"
                     /translation="MNKNESISYTEMQKFFAREIKEVGGRPITSKNLELSLLPIHARE
                     NLSAPEMPWYTEVNDIYMEFRRSPFWMVAGYVFLGFMLSLLSISILSIVAFSFYEMGF
                     NSDLLFLYLLAFVLGCFILPGAAKIAFFTPHYLPIRFNRKTQKIYVSDLIIEGNIFFG
                     KIKLTFHEYDWKDVEGWEVSRHFSKSMPYNGLHLVVNRENDINALRQTRVYTTRAPWS
                     AQEMARQKNYIPQIWLYCHNYMAYKTVSGAKNKPQPFIFLRNNWLFKWPKEMDKRSRS
                     SKILSE"
     gene            36459..36908
                     /locus_tag="QBH27_001553"
     CDS             36459..36908
                     /locus_tag="QBH27_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013878.1"
                     /translation="MKDNIHTAVIMAGEFLSANEWIRRKNQLKTSVHPASVASFAPYG
                     FNINQCDDDGNLWTNFPRPHIKLKIAPGKTACFLCRREELLFGYDEAHNHYQPNLNLV
                     LFVKNHGLHKDVYLKSHDDKSEYDSRILCNEKFTTYDFRKNRSRYSF"
     gene            complement(37093..37311)
                     /locus_tag="QBH27_001554"
     CDS             complement(37093..37311)
                     /locus_tag="QBH27_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDQ7013879.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKSDRQRGEVVGVEEQGKWHEE"
     gene            complement(37708..37986)
                     /locus_tag="QBH27_001555"
     CDS             complement(37708..37986)
                     /locus_tag="QBH27_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013880.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(38345..38635)
                     /locus_tag="QBH27_001556"
     CDS             complement(38345..38635)
                     /locus_tag="QBH27_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7013881.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(38636..38947)
                     /locus_tag="QBH27_001557"
     CDS             complement(38636..38947)
                     /locus_tag="QBH27_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ7013882.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            39176..40084
                     /locus_tag="QBH27_001558"
     CDS             39176..40084
                     /locus_tag="QBH27_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7013883.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(40148..41089)
                     /gene="fabY"
                     /locus_tag="QBH27_001559"
                     /gene_synonym="yiiD"
     CDS             complement(40148..41089)
                     /gene="fabY"
                     /locus_tag="QBH27_001559"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDQ7013884.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(41134..41571)
                     /gene="dtd"
                     /locus_tag="QBH27_001560"
     CDS             complement(41134..41571)
                     /gene="dtd"
                     /locus_tag="QBH27_001560"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDQ7013885.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQDMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(41568..42440)
                     /locus_tag="QBH27_001561"
     CDS             complement(41568..42440)
                     /locus_tag="QBH27_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDQ7013886.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(42434..43033)
                     /gene="yihX"
                     /locus_tag="QBH27_001562"
     CDS             complement(42434..43033)
                     /gene="yihX"
                     /locus_tag="QBH27_001562"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDQ7013887.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLRAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(43174..44061)
                     /locus_tag="QBH27_001563"
     CDS             complement(43174..44061)
                     /locus_tag="QBH27_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDQ7013888.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLTSPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(44051..45400)
                     /locus_tag="QBH27_001564"
     CDS             complement(44051..45400)
                     /locus_tag="QBH27_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDQ7013889.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
BASE COUNT        11534 a        11648 c        11152 g        11112 t
ORIGIN      
        1 ctgttggcct acacagtaaa gaaattacgc gaaagatgaa gtgtaatcag acctcttcca
       61 tgcgacccag cagggcctgc agacgttcct gccagccgtt ctgctgttct ttcagatggt
      121 tgttctcacg ctccagctct tcgcgctgat gctgggcatt ttgaacttcc tgcgacagtg
      181 agttgttttt ttctttcagc tcttcgattt ccatctgcaa cagagtgatg gtatcaatcg
      241 cctgctgtac ttttgcttcc agtttctcaa acacttctaa tgacattgtc atacctctcc
      301 tgaattgcaa ggcgttgatg gataaaaatc ctcgtcccga ttaccggtga cgccttaata
      361 aatacgagcg cactttagtt agctccgatt gtatgaagcc gcgccatcgc tgtccagcgg
      421 cacgccttgc agattacggt ttgccacact tttcatcctt ctcctggtga cataatccac
      481 atcaatcgaa aatgttaata aatttgttgc gcgaatgatc taacaaacat gcatcatgta
      541 caatcagatg gaataaatgg cgcgataacg ctcattttat gacggagcac acacatttta
      601 agttcgatat ttctcgtttt tgctcgttaa cgataagttt acagcatgcc tacaagcatc
      661 gtggaggtcc gtgactttca cgcatacaac aaacattaac tcttcaggat ccgattatga
      721 gtcaaacatc aaccttgaaa ggccagtgca ttgctgaatt cctcggtacc gggttgttga
      781 tttttttcgg tgtgggttgc gttgcagcac taaaagtcgc tggtgcgtct tttggtcagt
      841 gggaaatcag tgtcatttgg ggactggggg tggcaatggc catctacctg accgcagggg
      901 tttccggtgc gcatcttaat cccgctgtta ccattgcatt gtggctgttt gcctgtttcg
      961 acaagcgcaa agttattcct tttatcgttt cacaagttgc cggcgctttc tgtgctgcgg
     1021 ctttagttta cgggctttac tacaatttat ttttcgactt cgagcagact catcacattg
     1081 ttcgcggcag cgttgaaagt gttgatctgg ctggcacttt ctctacttac cctaatcctc
     1141 atatcaattt tgtgcaggct ttcgcagttg agatggtgat taccgctatt ctgatggggc
     1201 tgatcctggc gttaacggac gatggcaacg gtgtaccacg cggccctttg gctcccttgc
     1261 tgattggtct actgattgcg gtcattggcg catctatggg cccattgacg ggctttgcca
     1321 tgaacccagc gcgtgacttc ggtccgaaag tctttgcctg gctggcgggc tggggcaatg
     1381 tcgcctttac cggcggcaga gacattcctt acttcctggt gccgcttttt ggccctatcg
     1441 ttggcgcgat tgtaggtgca tttgcctacc gcaaactgat tggtcgccat ttgccttgcg
     1501 atatctgtgt tgtggaagaa aaggagacca caactccttc agaacaaaaa gcttcgctgt
     1561 aatatgacta cgggacaatt aaacatgact gaaaaaaaat atatcgttgc gctcgaccag
     1621 ggcaccacca gctcccgcgc ggtcgtaatg gatcacgatg ccaatatcat tagcgtgtcg
     1681 cagcgcgaat ttgagcaaat ctacccaaaa ccaggctggg tagaacacga cccaatggaa
     1741 atctgggcca cccaaagctc cacgctggta gaagtgctgg cgaaagctga tatcagttcc
     1801 gatcaaattg cagctatcgg tattacgaac cagcgtgaaa ccactattgt ctgggaaaaa
     1861 gaaaccggca aacctatcta taacgccatt gtctggcagt gccgtcgtac cgccgagatc
     1921 tgcgaacatc tcaaacgcga tggcatggaa gagtacattc gtaacaacac cggtctggtg
     1981 attgacccgt acttctctgg caccaaagtg aagtggatcc tcgaccatgt ggaaggctct
     2041 cgcgagcgtg cgcgtcgtgg tgaattgctg tttggtacgg ttgatacgtg gcttatctgg
     2101 aaaatgactc agggccgtgt ccatgtgacc gattacacca acgcctctcg taccatgttg
     2161 tttaacatcc atactctgga ctgggacgac aaaatgctgg aggtgctgga tattccacgc
     2221 gagatgctgc cagaagtgcg tcgttcttcc gaagtatacg gtcagactaa cattggcggc
     2281 aaaggcggca cgcgtattcc aatctccggg atcgccggtg accagcaggc ggcgctgttt
     2341 ggtcagttgt gcgtgaaaga aggaatggcg aagaacacct acggtaccgg ctgctttatg
     2401 ctgatgaaca ctggcgagaa agcggtgaaa tcagaaaacg gcctgctgac caccatcgcc
     2461 tgcggcccga ctggcgaagt aaactatgcg ctggaaggtg cggtgtttat ggcgggcgcg
     2521 tccattcagt ggctgcgcga tgagatgaag ttgattaacg acgcctacga ttccgaatat
     2581 tttgccacca aagtgcaaaa caccaatggt gtgtatgtgg ttccggcctt taccgggctg
     2641 ggtgcgccat actgggatcc gtatgcacgc ggggcgattt tcggtctgac tcgtggggtg
     2701 aacgctaacc acattatacg cgcgacgctg gagtctattg cttatcagac gcgtgacgtg
     2761 ctggaagcaa tgcaggccga ctccggtatt cgtctgcacg cgctgcgcgt ggacggcggt
     2821 gcggtggcga acaatttcct gatgcagttc cagtccgata ttctcggtac gcgcgttgag
     2881 cgcccggaag tgcgcgaagt caccgctttg ggtgcggcct atcttgctgg tctggcggtt
     2941 ggcttctggc agaatcttga cgagctgcaa gagaaagcgg tgattgaacg cgagttccgt
     3001 ccaggcatcg aaaccactga gcgtaattac cgttacgcag gctggaaaaa agcggtcaaa
     3061 cgcgcgatgg catgggaaga acacgacgag taataatgat tgcctgatgc gacgcttgcg
     3121 cgtcttatca ggcctacgag tcggatacac atccggcatc acgcagactc tgaaaccgtg
     3181 cgggggcaac cccgcacaca tcaataatcc cgcccttccc ctgtgctaca cttcgcgcca
     3241 ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt tcccgcgtca
     3301 ccgaatcagc ggcgctggct ggctacaaat ggttaggacg cggcgataaa aacaccgcgg
     3361 acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt gacggcacca
     3421 tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt gaaaaagtcg
     3481 gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc acgcgcatga
     3541 cggcgatggg ccaggctaac gcgctggcgg tgctggcagt aggcgataaa ggctgtttcc
     3601 tcaacgcgcc agatatgtat atggagaagc tgatcgtcgg accgggagcc aaaggcacca
     3661 ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt tgcggcggcg ctcggcaaac
     3721 cgttgagcga actgacggta acgattctgg caaaaccgcg ccacgatgcc gttatcgctg
     3781 aaatgcaaca actcggcgta cgcgtatttg ctattccgga cggcgacgtt gcagcctcaa
     3841 ttctcacctg tatgccggac agtgaagtcg atgtgctgta cggcattggc ggcgcgccgg
     3901 aaggcgtggt ttctgcggcg gtgatccgcg cattagatgg cgacatgaat ggtcgtctgc
     3961 tggcacgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc gagcaggagc
     4021 tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt attgcgtttg ggcgatatgg
     4081 cgcgcagcga caacgtcatc ttctctgcca ccggtattac caaaggcgat ctgctggaag
     4141 gcattagccg caaaggcaat atcgccacca ccgaaacgct gctgatccgt ggtaaatcac
     4201 gcaccattcg tcgcattcag tccatccact atctggatcg taaagacccg gaaatgcagg
     4261 tgcacattct ctgagtgatt tgatcgattg agccttccag tccttcggga ctggaatttt
     4321 tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt
     4381 gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt agtctcaccg
     4441 ttcacgctcc cgtgcttccg tttaccgccg ggcaattcac caaacttggc cttgaaattg
     4501 aaggcgaacg cgttcagcgt gcctactctt acgttaactc gcccgacaat cccgatctgg
     4561 agttttacct ggtcaccgtc cccgatggca aattaagccc gcgactggcg gcgctaaaac
     4621 cgggtgatga agtgcaagtg gttagtgagg cggcaggctt ctttgtgctg gatgaagtcc
     4681 ctgactgcga aacactgtgg atgctggcga ctggtacggc gattggccct tatttatcga
     4741 ttctgcaatt gggcaaagat ttagaacgct tcaaaaacat ggtgctggtt cacgccgcac
     4801 gatatgccgc cgacttaagc tatctacctc tgatgcagga actggaaaaa cgctacgagg
     4861 ggaaactgcg cattcagacg gtggtcagtc gggaaacggc tgcgggttcg ctcactgggc
     4921 ggataccggc gttaattgaa agtggtgaac tggaaagcgc ggttggcctg ccgatgaata
     4981 aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat acacaacagt
     5041 tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggc catatgacag
     5101 cggagcatta ctggtaagcg gttacttgtc gataaacggc acgatcaaga gatccgcact
     5161 caacttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg agtggtgatg
     5221 accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg tttccggcat
     5281 ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg tcagtttata
     5341 cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg cagggaagta
     5401 gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca ggtgagcgtc
     5461 attatgtctg gcgagctcca gggctttatt caccagtaag gcatcttctt cattcccgga
     5521 aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca actccatcaa
     5581 gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga gaaggtgaag
     5641 gccgcatcaa gctcaacttg cccttccaga tctttaatct tataggccac gctggcccct
     5701 tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa ggactctgct
     5761 tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc
     5821 gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc tttcataggg
     5881 cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc cgttaaagga
     5941 agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgct taaggtgaag
     6001 agttcagaag gattttccgg gcattgtcac ttgcatcgtg gaaataacaa tcccggaaaa
     6061 aaatagcgat aagagatata ttaaaggtta attacttgat ttattgtcgg ctttatactt
     6121 cacatcctgg gtatctttac catatttatt ttcgccttgt gtgccaacaa acgcaccaag
     6181 atcgataagc atcatcacca gaatcagtgt tgggataaaa cgccccaccg cccattgcca
     6241 tacccccggc aaaattgccc agttacccgc cagcagcatc cacgccacaa tcatcagaaa
     6301 tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta acggttactg ccgctgttgg
     6361 ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata tcgagtaaat ttttacccgc
     6421 cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg
     6481 gcgtccaata cgccctttaa atgagaataa ccattgctgt atggtcatgt aagttccttg
     6541 atggttgtct tttccaggat tctacccttt tgacaagggc gacagatatc gttttaatcg
     6601 gagccagtca taacaaaagg tactgtcaat gaagccaggg tgtacgctgt ttattctctt
     6661 atgttctgca ttaaccgtta caacaacggc gcatgcacaa acaccagata cggcaacgac
     6721 cgcgccttat ctgctggctg gagcccctac tttcgatctc tccatcagcc agtttcgaga
     6781 agactttaac agccagaatc ccagcctgcc actgaacgaa tttcgtgcca tcgacagcag
     6841 tcccgacaaa gccaatctca ctcgtgcagc aagtaaaatt aacgagaact tgtatgcttc
     6901 tactgcgctg gagcgcggta ccttaaaaat caaaagcatt caaataacct ggctacccat
     6961 ccaggggcca gagcaaaaag ccgcgaaagc gaaagctcag gcatacatgg cagcggtgat
     7021 ccgcacactc accccattaa tgaccaaaac tcaaagccag aaaaaactgc agtcgctact
     7081 aacggcgggg aaaaacaaac gttattacac cgagacagaa ggtgcactgc gttatgttgt
     7141 cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc tggcgctatc
     7201 tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc
     7261 tatactgttt cacagacctg ctgccctgcg gggcggccat cttcctttat tcgcttataa
     7321 gcgtggagaa ttaaaatgcg acatccttta gtgatgggta actggaaact gaacggcagc
     7381 cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag agctggcagg tgttgctggc
     7441 tgtgcggttg caatcgcacc accggaaatg tacatcgata tggcgaagcg tgaagctgaa
     7501 ggcagccaca tcatgctggg tgcgcaaaac gtggacctga acctgtccgg cgcattcacc
     7561 ggtgaaacct ctgctgctat gctgaaagac atcggcgcac agtacatcat catcggccac
     7621 tctgaacgtc gtacttacca caaagaatct gacgaactga tcgcgaaaaa attcgcggtg
     7681 ctgaaagagc agggtctaac tccggttctg tgcatcggtg aaaccgaagc ggaaaacgaa
     7741 gcgggcaaaa ctgaagaagt ttgtgcacgt cagatcgacg cggtactgaa aacccagggt
     7801 gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat cggtactggc
     7861 aaatctgcaa ctccggctca ggcacaggct gttcacaaat tcatccgtga ccacattgct
     7921 aaagttgacg ctaacatcgc tgaacaagtg atcattcagt acggcggctc tgtaaacgcg
     7981 tctaacgctg cagaactgtt tgctcagcca gacatcgacg gcgcgctggt tggcggtgct
     8041 tctctgaaag ctgacgcttt cgccgtgatc gttaaagctg cagaagcggc taaacaggct
     8101 taagtctgac aggtgccgga tttcatatcc ggcacttact ttcctaaact cctcgcctta
     8161 acgcaaaatc tcacactgat gatcctggat ttcttcggct gaagcacggt tcagcgtcag
     8221 tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca ctctgccgta ccatcgccag
     8281 cccgtagctt cccatatgtt cccgcgcctc aggcacttct tccgccagca tcataaatgg
     8341 gctgcgttgt accagttcgc tttccgttac ccgacgcgcc aggtactcat gcccacgcaa
     8401 accacctggc agtggcaacc agcggctact gatgttcgcc agattgttat ccagctgttc
     8461 gcgcacatca ggacgaatac aagaaatatg aatatgaaaa tggttttgcg tacgcccggt
     8521 gcgggagttg atcgccagag ataccgcgcg atcgggaaca ggctggccgt atttttggct
     8581 cataaaatca cgcgcctgcc aggctaacca gaaaaaatta ggcgttgacg gatcggtcaa
     8641 caatgggctt tcagtaccgt taatacggta cgttggcatt aacagatatt gcagtgggcc
     8701 atgacgatct tttaaaacca cgtatccggc gttgggtttg acttccgcac atggcgacgg
     8761 gttttggttt tcctgctggt tgggcaaaca ttgctcaaga actatcttac gtaacgtgtc
     8821 tgactcttca ccggttaatt tccagtaacc aataccggca gtcacaacgg cgataactat
     8881 catcaccaaa aaaagaagac ccgctttttt catctttttt tccctgtacc tcaaagagat
     8941 gcaagggtaa cgcaaaatcg tgacaaataa aaaacccagt ggcgagaaac caccgggctt
     9001 gataattatc ctggggaaat cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa
     9061 aatgctcttt ttgcgctttc gtccagccgc cgaactcttc atcaatggtg aataacttca
     9121 attttggaaa cgcattttcg tactttttcg ctacctcagc gtcgcgcgga cggtagtagt
     9181 ttttcgctgc aatttcctga ccttctggcg agtagagata tttcaggtag gcttccgcca
     9241 cctctttggt gccttttttc tcgaccactt tatcgaccac cgacacggtt ggctctgcga
     9301 ggatagactc actcggcgtg acgatttcga atttatcttt ccccagttca ttcgctgcca
     9361 gcagagcttc gttttcccag gcaatcagta catcgccaat tccgcgctcg acaaaagtgt
     9421 tagtggatcc acgcgcgcca gaatccagaa cttcgacgtt tttatacagt gcccgaacaa
     9481 aatcctgtgc ttttgcctga tcgttgttgt tgtgatgaag cgcgtagccc caggctgcca
     9541 ggtaattcca gcgcgcgcca ccagagcttt tcggattagg cgtgatcacc gaaacacccg
     9601 gtttaatcag atcgttccag tcatggatct gcttcggatt acccttacgt accaggaaaa
     9661 caatggtgga agtgtacggt gcggagttat ccggcagacg tttgatccac tctttatcaa
     9721 tccgcccgcg ttccgcaatt gcgtccacgt cataggccag agccagcgtg acaacatcag
     9781 cttcaatacc gttgattacc gacgtcgctt gtttgcctga accaccgtgt gactgacgaa
     9841 tcaccacgtt atcgccagtt tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt
     9901 cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc tttgccataa
     9961 cgctggttgc caccagcaaa aatgttaacc ctacgcccca ctttttcatc gcccgactct
    10021 cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt gaccaaacat taaagaataa
    10081 aaaaagattg gctataactt gcgagtatat gttgggggat taaaaaggcg gaagaaatcc
    10141 gcctcatatt gctgacaaag tgcgctttgt ccatgccgga tgcggcacaa acgccttatc
    10201 cggcctacaa aagtttgcaa attcaataga ttacagaatt catgtaggcc tgataagcga
    10261 agcgcatcag gcctttttgc ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc
    10321 cgaaatcatt aatacagttt tttcgcgcag tccagccagt cgcctttgaa cggacgcttc
    10381 atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct gttcgttctg gatacctacg
    10441 caacgaccac cgtaacctgc cagcagcaga tcgatagcgt aagcgcccat acgggaagcc
    10501 agaatacggt cgtaaggcac cggagaacca ccgcgctgga tgtggcccag cacagttgcg
    10561 cgggtttcac gaccggtttc tttctcgatg aaatgcgcca gttcgtcaac atcacacata
    10621 tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg
    10681 tttaccaggt cttcacggct gaattcaact tccggaacca caacgaattc acagccaccg
    10741 gcaatggccg cagccaacgt cagatcgcca caataacggc ccatcacttc caccacggaa
    10801 atacgctggt gagaagaaga ggtgtcacgc agacggtcga tcgcttctac aacggtgctc
    10861 agcgcagtga agaaaccgat agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc
    10921 ggcaggccga tgcacgggaa gcccatttcg gtcagacgca ttgcacccat gtaggaaccg
    10981 tcaccgccga taaccaccag cgcgtcgatc ccacgttttt tcaggttttc gatagccacg
    11041 gcgcggatgt tctcgtcgcg gaattccggg aaacgcgcag aaccgaggaa cgtaccgcca
    11101 cggttgatca tgtcagaaac gctgtaacgg tctagctgta ccatacggtc ttcatacaga
    11161 cccagatagc cgtcataaat gcccattact tccagacctt ctgtcagcgc agaacgaaca
    11221 accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt
    11281 ttcttaatca tgactacctc tgaactttgg aatgcaaaat gaaatctgtt gccggaagtc
    11341 ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt ataacaatca cttcctgctg
    11401 aattgattca ggtcaggcca aatggcggta ttttatacac aaaatgcggg tctggctctc
    11461 ttttatactg attatgaaag catagaccgt ttaccctccc tgggtacgac ggaacagggg
    11521 tcctgatgga taatcacatc cgatcccgga aaacgccgta aaatagcctg ctctacctga
    11581 tccgccacca tatgtgcctg aaccaaaggc agagagtctt ccatttccaa atgaatctga
    11641 ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc
    11701 caggaagtca cgatatcaat aatttcttgc cgttcctcat caggcaatgc gcgatccagt
    11761 aatgactgta ccgcctcata tcccatgcgt aacgcgctat ataaaatata gatgccgatc
    11821 cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc
    11881 agaattgcgc cgttcatcat aacatcagac tggtaatgta gcatatcagc ccgcaccgcc
    11941 tggctttgcg tccggcgcac cacccaacgc tgaaacgaga caaggataat cgtacaaatt
    12001 agcgccacaa ttgtcacgat aaccccgacg cctggatctg tcatcggtgt tggagatacc
    12061 agatgttgaa tacccgtcaa aaacaggaat agtgccgaac cggagataaa catactttgc
    12121 gccagcgccg cgagggactc agctttaccg tgaccaaacg agtgattatc gtcggcaggt
    12181 tgcagggaat atcgcaccac cagtaaattc gtcaacgacg caccgatatc caccagcgaa
    12241 tccaccagcg cggcgagaat actcaccgac ccggtatacc accatgcaaa aattttaatc
    12301 agcaatagca gcgaagccat cgccgtcgca gcaatcgccg cccgactgac cagccgtcca
    12361 taagattgat tcataaatac tcccgctatc aactgacggt agtataacgg aagcaaatca
    12421 tctgcaatgc attaagcagc aggcaaattg aggataaaaa aaacccccac atcatgtggg
    12481 ggaagacagg gatggtgtct atggcaagga aaacagggtt tactactggg aacgtgagtt
    12541 gctactactc aatagcttca acgatgaact tttttgccat tgcgtcacgt cgcgcaactg
    12601 ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg
    12661 atacatttgg ttgcggactt ttgccatctc aacctgacga gcaatttgct cattcgccat
    12721 tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt tctgcggtga caaggcgatg
    12781 cattgtctcc agttcgctaa cattaacagg aggctgttcg tgccgggcct gttgcataag
    12841 atctcgcatc tgctgacgct gatgttcggt taaacttatg ccgtcgaaca tatggctctg
    12901 cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta tcgcctgaac cgacttcagc
    12961 agcgtggctt aatgaactga ctgccagcgt tgaggccatg acggcagcgg taactatgcg
    13021 catcatttgc tcccaaaatc tttctgtcgc gattcaacga tagagagttt acgattcagg
    13081 ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc atggattagc gacgtctgat
    13141 gacgtaattt ctgcctcgga ggtatttaaa caatgaataa aatcctgtta gttgatgatg
    13201 accgagagct gacttcccta ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg
    13261 ttgcccacga tggggaacag gcgcttgatc ttctggacga cagcattgat ttacttttgc
    13321 ttgatgtaat gatgccgaag aaaaatggta tcgacacatt aaaagcactt cgccagacac
    13381 accagacgcc tgtcattatg ttgacggcgc gcggcagtga acttgatcgc gttctcggcc
    13441 ttgagctggg cgcagatgac tatctcccga aaccgtttaa tgatcgtgag ctggtggcac
    13501 gtattcgcgc gatcctgcgc cgttcgcact ggagcgaaca acagcaaaac aacgacaacg
    13561 gttcaccgac actggaagtt gatgccttaa gtctcaatcc tggtcgccag gaagccacct
    13621 tcgacgggca aacgctggag ttaaccggta cggagtttac cctgctctat ttgctggcac
    13681 agcatctggg tcaggtggtt tcccgtgagc atttaagcca ggaagtgctg ggcaagcgcc
    13741 tgacgccttt cgaccgcgct atcgatatgc acatttccaa cctgcgtcgt aaactgccgg
    13801 atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt
    13861 ctgcttcatg ataggcagct taaccgcgcg catcttcgcc atcttctggc tgacgctggc
    13921 gctggtgttg atgttggttt tgatgttacc caagctcgat tcacgccaga tgaccgagct
    13981 tctggatagc gaacagcgtc agggtctgat gattgagcag catgtcgaag cggagctggc
    14041 gaacgatccg cccaacgatt taatgtggtg gcggcgtctg ttccgggcga ttgataagtg
    14101 ggcaccgcca ggacagcgtt tgttactggt gaccaccgaa ggccgcgtga tcggcgctga
    14161 acgcagcgaa atgcagatca ttcgtaactt tattggtcag gccgataacg ccgatcatcc
    14221 gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga
    14281 agataattac caactttatc tgattcgtcc ggccagcagt tctcaatccg atttcataaa
    14341 cttactgttt gaccgcccgc tattactgct gattgtcacc atgctggtca gtacgccgct
    14401 gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt aagctgaaaa acgctgccga
    14461 tgaagttgcc cagggaaact tacgccagca cccggaactg gaagcggggc cacaggaatt
    14521 ccttgccgcc ggtgccagtt ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc
    14581 tcagcagcgt ctactttctg atatctctca cgaactgcgc accccgctga cgcgtctgca
    14641 actgggtacg gcgttactgc gccgtcgtag cggtgaaagc aaggaactgg agcgtattga
    14701 aaccgaagcg caacgtctgg acagcatgat caacgatctg ttggtgatgt cacgtaatca
    14761 gcagaaaaat gcgctggtta gcgagaccat caaagccaac cagttgtgga gtgaagtgct
    14821 ggataacgcg gcgttcgaag ccgagcaaat gggcaagtcg ttgacggtta acttcccacc
    14881 tgggccgtgg ccgctgtacg gcaacccgaa cgccctggaa agtgcgctgg aaaacattgt
    14941 tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg ggctttgcgg tagataaaga
    15001 cggtatcacc attacggtgg acgacgatgg tcctggcgtt agcccggaag atcgcgaaca
    15061 gattttccgt ccgttctatc ggaccgatga agcacgcgat cgtgaatctg gcggtacagg
    15121 tttggggctg gcgattgttg aaaccgccat tcagcagcat cgtggctggg tgaaggcaga
    15181 agacagcccg ctgggcggtt tacggctggt gatttggttg ccgctgtata agcggtcata
    15241 acgtcaacct tcaggtaaaa aaatgcctga tgcactacgc ttatcaggcc tacaaaacct
    15301 gttgaattta tgggttttgt aggctggata aggcgtttac gccgcatccg gcatttgagc
    15361 aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg gttattttcc
    15421 ccacaaacgg cgggaaaaat cttcgatctt gccgctcagt cggcgctttt gcatcgttcg
    15481 cgtccagctt ttcgataacc ctgcaacgga gagtaaacgg tgatactgat cgtcatcaaa
    15541 cggcatatgc catgcgatgg cggcagcctc ctgcacggtc acatcgctga cacgggaaac
    15601 caactccagt ggcgcatcag cagataccaa ccctggtgca attacgctat acaaccaccc
    15661 cactttaccc gtgttttgca tcaactgcgc tatatcgctg atatcaaaat ggtaattgag
    15721 tttatagcaa ggtgagcgtg gctggctgac ctgaattaat gcctctcccc agcggaaaat
    15781 atcaccaata taaacattac tttccgtcag accgtcggtt gagagatttt cgccaaacgc
    15841 aggcgcaaca aacagctccg cctgttccgg aaattcccgc gcccagtaga gataatgctc
    15901 acgaggataa tgacacagcg ctctgtctgg cccgccgtgg atctttttct ccgcctgttc
    15961 gtcaccttcc agccccaggt ccgtcagcat caactcacca tcaacctgga ttttggcaat
    16021 tgcgctgggt ttgccttcgg gataagcctg aatcttgcct gtgtatacat caaccggata
    16081 tcgcatctgt tccctctcca cgagaaattt ccccattaga ccatatttat tttatgtcag
    16141 gcgtgctgtt ttcgcggtaa gtatccaggc agaaacgcaa ttgaaaaccg gacgaacgtt
    16201 agttcatccg gctgtgggga ttaaggaagc tggtttattt caccgtacct aaaatttcga
    16261 ttttcgccgc tctggctttg actttacgtg accagataga ggtaagaatt ggcaccagaa
    16321 tcgaagtcac aatgaccgcc gtcgctacca gcgaagttgc tgccggagcc ataggtttaa
    16381 acgcaggcac catttctgca atcagcacag gtgtcgctac cgcggcccca gcggaactgg
    16441 aagcggcaat tccggcagtg ccatcgccac cgccaatcaa tttatcggcg ataatcaacg
    16501 gaataccggt cacgataatt actgccacac ccaacaggat ccccagcaaa cctgtctggg
    16561 caattacagt caaatcaatg gtattgccca gtgcgaaggc aaagaatgga atcagcgtct
    16621 gcaccgcttt gctgaaaaat tctcgtaact cagggtcaag gttccccagg gcaaagccca
    16681 ccaggaacgg caatactgcg ccgacgaaaa catgcggttc aaacgaggca atcccggcag
    16741 tgcccagaat aatcatcgtc atgagcggcc cggactccaa cgacatcaac acaaatgccc
    16801 cagcttcttc ttttgtgccg tactgctgca tgatggaagc gtaaagtccg ccgttggtca
    16861 tatccatcgc cgccaccagc gccaacgttg aaagtccggc aaaaaatcca acttcaacac
    16921 catgttccgg aataatgcgc gaggcaattg ccgcaaccac ccacgcgacg gcaattttag
    16981 ttaccaccag cgtaccggat ttacgcagta ccgttcccgt cgcgcttaat tttattgacg
    17041 cccccatgca aaaaaaccac accgccagaa tgggcaccgt accggtaatc atcccgttgg
    17101 tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt gtggcacagt gcgccaagga
    17161 atagcgggac gagcatcatc ccccccggga ttttctcaat cgagcgtttt atctgcattt
    17221 ccatcacctg tcggcatatt tttaagggct gatgcgtcat tcctcaacgg agcatcagtg
    17281 ttgatggaaa cgaagataaa cattcatttg atcaataaaa gtgatcaaaa ccacatcaat
    17341 tgaaacgatg ttttattttt atcggatcgt tgtttttatt ttgaaagtga tcacaaaaaa
    17401 accaccgccg ttggcgatgg ttcatttttt aagctgcaat gcggcgtaaa cgcctcattg
    17461 cagcaggcgt caaatgatta ttttttcgcc gcgaaacgtg ccgctgcttc gtcccagttc
    17521 accacgttcc agaactcttt aatgtagtcc ggacggcggt tctggaattt caggtagtaa
    17581 gcatgttccc acacatccag gcccacaatc gggaagccgg aagcgccaga aatggcttcg
    17641 cccatcagcg gagagtcctg gttagcagta gaaaccaccg ccagtttatc gcctttttgc
    17701 accagccatg cccagccgga accaaagcgg gaagctgccg ctttttcaaa ttctgctttg
    17761 aaattatcaa cggaaccgaa atcacgttcg atagccgctt tcaggtcgcc ctgcagggtg
    17821 gtgccttttt tcagaccttt ccagaacaga ctgtggttag cgtgaccgcc agcgttattg
    17881 cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt tggtgatcag ctcttcaacc
    17941 ggcaggttag cgaattctgg caggctttcc agcgccgcat tggcgttgtt aacgtaggcc
    18001 tgatggtgtt tggtgtggtg gatttccatg gtctgcttat cgaagtgcgg ttccagggca
    18061 tcgtaagcat acggcaggga tggcagggta taactcatat tcatctccag tgtggtcggg
    18121 cggccgattg ttaatgccgc gtaagcagtt ggttcattat agttaattaa atgatattga
    18181 aaatgattat caatgccgta cttttcataa gggtatggtt ttgcaggaaa atgcccaaga
    18241 tgtgaagcaa atcacccact taatgccgtg attgccagta aatcggcagc ggcggcaaca
    18301 ggcgaaaggt taatcgacag ctcaattttt acactcatct cgtcggagat gtgacgcgac
    18361 gaaaaatgat gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt
    18421 gatcggcgcg gccagtgcag cctgttttta cgcgccgttc aaaaaagtaa aaaagtggtc
    18481 atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg attatcctgc cgtgggccat
    18541 cagcgccctg ttactaccga atttctgggc gtattacagc tcatttagtc tctctacgct
    18601 gctgcctgtt tttctgttcg gcgctatgtg ggggatcggt aatatcaact acggcctgac
    18661 catgcgttat ctcggcatgt cgatgggaat tggcatcgcc attggcatta cgttgattgt
    18721 cggtacgctg atgacgccaa ttatcaacgg caatttcgat gtgctgatta ataccgaagg
    18781 cggacgcatg acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg
    18841 cgccgggcag ttgaaagagc gcaagatggg cattaaagcc gaagagttca atctgaaaaa
    18901 agggctggtg ctggcggtga tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa
    18961 cgccgcaaaa ccgatgcatg aagccgctgc cgcgctgggc gtcgatcccc tgtatgtcgc
    19021 tttgccaagc tatgttgtca tcatgggcgg cggcgcaatc atcaacctcg gcttctgctt
    19081 cattcgtctg gccaaagtga aagatttgtc gctaaaagcc gacttctcgc tggcaaaacc
    19141 gctgatcatt cacaatgtgt tactctcagc actgggcggg ttgatgtggt atctgcaatt
    19201 ctttttctat gcctggggcc acgcccgcat tccggcgcag tatgactaca tcagctggat
    19261 gctgcatatg agcttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg
    19321 gaacaatgca ggacgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt
    19381 cgccgctaac atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg
    19441 ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc
    19501 ttcaaagcca cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata
    19561 ttgcgcatga cacactctga cctgccgcag atattgattg atggtcattc cagtctgctg
    19621 gcgaaattgc tgacgcaaaa cacgctcact gcatgatgcc tcatcacaaa atttatccag
    19681 cgcaaaggga cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaatgtttc
    19741 gctggatgtt ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa
    19801 ctgcccgaac agcaactcag ccatttcgtt agcaaacgac acatgctgac tactttcatg
    19861 ctcaagctga ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg
    19921 tggttgccct gcgctggcgc taaatcccgg aatcgcgccc tgccagtcaa gattcagctt
    19981 cagccgctcc gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga
    20041 gtgtttatcg tcagcatgaa tataaaagag atcgccacgg gtaatgcgat aagggcgatc
    20101 gttgagtaca tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt
    20161 atgttcggca aagacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct
    20221 ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag
    20281 agaacgaagt tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt
    20341 gcagaaaacc atcccgtccc tgacgaatat cacgcggtga ccagttaaac tctcggcgaa
    20401 aaagcgtcga aaagtggtta ctgtcgctga atccgcagcg ataggcgatg tcagtaacgc
    20461 tggcctcgct gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg ttcaggtaac
    20521 gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc aatgaaagag
    20581 aaaattgagc cgccactgca tcccaattca cctcatcggc aaaatggtcc tccagccagg
    20641 ccagaagcaa gttgagacgt gatgcgctgt tttccacgct ctcctgcaaa ctgcttttac
    20701 gcagtaagag cagtaattgc ataaacagaa tttcacgact ggcggtcgag ggtaaatcat
    20761 ttccctcttc ttgttgttcc aactgtgcaa ccagttggcg cacctgctgt aatacgcaat
    20821 ggttaacgcg ccagtgagac ggatactgcc catcctgctc ttgtggcagc aactgattca
    20881 gcccggcgag aaactgaaat cgatccggcg agcgatacag cacattggtc agacacagat
    20941 tatcggtatg ttcatacaga tgccgatcat gatcgcgtac gaaacaaacc gtcccaccgg
    21001 tgatggtata gggctgccca ttaaatacat gaatacccgt gccatgttcg acaatcacaa
    21061 tttcatgaaa atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg
    21121 ccacggacgc gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct
    21181 cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt
    21241 cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa
    21301 tgcggtgagc atcacatcac cacaatgaag caaattgtga acatcatcac gttcatcttt
    21361 ccctggttgc caatggccca ttttcctgtc agtaacgaga aggtcacgaa ttcaggcgct
    21421 ttttagactg gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc
    21481 gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc
    21541 cgcagcctga cgctgcgcga aatccatcgt tttaacaatg gcctgcatag ccagaacggt
    21601 tatgtcacct ggaatgtgga tagcctggaa agtgccattc gccttggatt aaacaaggtg
    21661 tgcgaggaag ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg
    21721 ctgctcgacc agcacggtca gcgcgtgggt ctgcccgttg cttaccgcga tagccgcacc
    21781 aatggcctaa tggcgcaggc acaacagcaa ctcggcaaac gcgatattta tcaacgtagc
    21841 ggcatccagt ttttgccctt caatacgatc tatcagctac gtgcactgac agagcagcaa
    21901 ccagaactta ttccacacat tgctcacgct ttgctgatac cggattactt tagttatcgc
    21961 ctgaccggca agatgaactg ggaatatacc aacgccacga ccacgcaact ggtcaatatc
    22021 aatagcgacg actgggacga gtcgctactg gcgtggagcg gtgccaacaa agcctggttt
    22081 ggtcgcccga cgcatccggg taatgtcata ggtcactgga tttgcccgca gggtaatgag
    22141 attccggtgg tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta
    22201 aacggttcac gcgccgctta tctctcttct ggcacctggt cattgatggg cttcgaaagc
    22261 cagacgccat ttaccaatga cacggcgctg gcagccaata tcaccaacga aggcggggcg
    22321 gaaggtcgct atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgagtgctt
    22381 caggagcggc aaatcaacga tcttccggcg cttatcgccg caacacaggc acttccggcc
    22441 tgccgcttca tcatcaatcc caatgacgat cgctttatta atcctgacga gatgtgcagc
    22501 gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc cggaaagtga tgctgaactg
    22561 gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg atgtgttgca tgagctggcg
    22621 cagctgcgtg gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg
    22681 ctgctcaacc agctgtgtgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa
    22741 gcctcgacgc tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg
    22801 gatgatttcc gtcaggtcgt cagcactacc gcgaatctga ccacctttac ccctaatcct
    22861 gacagtgaaa ttgcccacta tgtggcgcag attcactcta cacgacagac aaaggagctt
    22921 tgcgcatgac cactcaactg gaacaggcct gggagcttgc gaaacagcgt ttcgcggcag
    22981 tagggattga tgtcgaggag gcactgcgcc aacttgatcg tttacccgtt tcaatgcact
    23041 gctggcaggg cgatgatgtt tccggttttg aaaacccgga aggttcgctg accggtggta
    23101 ttcaggctac tggtaattat ccgggcaaag cgcgtaatgc cagcgagctg cgtgccgatc
    23161 tggaacaggc tatgcggctg attccggggc caaaacggct taatttgcat gccatttatc
    23221 tggagtcaga cacgccagtc tcacgtgacc aaatcaaacc agagcacttc aaaaactggg
    23281 ttgaatgggc gaaagccaat cagctcggtc tggattttaa cccctcctgt ttttcacatc
    23341 cgctaagcgc cgatggcttt acactttccc atgccgacga ccgcattcgc cagttctgga
    23401 ttgaccactg taaagccagc cgccgtgttt cagcctattt tggtgagcaa ctcggcacac
    23461 cgtcggtgat gaacatctgg atcccggatg gcatgaaaga tatcaccgtt gaccgtctcg
    23521 ccccgcgtca gcgtttgctg gcagcactgg atgaagtgat cagcgagaag ctaaaccctg
    23581 cgcaccatat cgacgccgtt gagagcaaat tgtttggcat tggcgcggag agctacacgg
    23641 ttggctccaa tgagttttac ctggggtatg ccaccagccg ccagacggcg ctgtgcctgg
    23701 acgccgggca tttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt
    23761 atgtgccgca gttgctgctg cacgtcagcc gtccggttcg ctgggacagc gatcacgtag
    23821 tgctgctgga tgatgaaacc caggcgattg ccagtgagat tgttcgtcac gatctgctcg
    23881 accgggtgca tatcggcctc gacttcttcg atgcctctat caaccgcatt gccgcgtggg
    23941 tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg
    24001 agctacgcaa gctggaagcg gcgggcgatt acactgcgcg tctggcgcta ctggaagagc
    24061 agaaatcgtt gccgtggcag gcggtatggg aaatgtattg tcagcgtcac gatacgccag
    24121 cgggtagcga atggctggag aacgtgcgga cttatgagaa aaaaattttg agtcgccgcg
    24181 ggtaaacact gccggatgcg gcacaagtgc cttatccggc ctacgggtcg gcaatagttg
    24241 taggcctgat aagacgcgac agcgtcgcat caggcattga ttgccggatg cggcacgagt
    24301 gccttatccg gcctacgggt cagcaacagt tgtaggcctg ataagacgcg acagcgtcgc
    24361 atcaggcatt gattgccgga tgtggcgcga gtgccttatc cggcctacag gtcgtgcatc
    24421 cgacaacacc gaattaacag gaacacagaa catgcaaaac ataacccagt cctggtttgt
    24481 ccagggaatg atcaaagcca ccaccgatgc ctggctgaaa ggctgggacg agcgcaacgg
    24541 cggcaacctg acgctgcgcc tggatgacgc cgatatcgca ccatatcacg acaattttca
    24601 ccaacaaccg cgctatatcc cgctcagcca gcccatgcct ttactggcaa atacaccgtt
    24661 tattgtcacc ggctccggca aattcttccg taacgtccag cttgatcctg cggctaactt
    24721 aggcatcgta aaagtcgaca gcgacggcgc gggctaccac attctttggg ggttaaccaa
    24781 cgaagccgtt cccacttctg aacttccggc tcactttctc tcccactgcg agcgcattaa
    24841 agccaccaac ggtaaagatc gggtgatcat gcactgccac gccaccaacc tgatcgccct
    24901 cacctatgta cttgaaaacg acaccgcggt cttcactcgc caactgtggg aaggcagcac
    24961 cgagtgtctg gtggtattcc cggatggcgt tggcattctg ccgtggatgg tgcccggcac
    25021 ggacgaaatc ggccaggcaa ctgcacaaga gatgcaaaaa cattcgctgg tgttgtggcc
    25081 cttccacggt gtgttcggca gcgggccgac gctggatgaa accttcggtt taatcgacac
    25141 cgcagaaaaa tcagcacaag tattagtgaa ggtttattcg atgggcggca tgaaacagac
    25201 catcagccgt gaagagttga tagcgctcgg ccagcgtttc ggcgttacac cactcgccag
    25261 tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc aggtaaaccc
    25321 cgacgcccac gaagagtatc agcgtcggca taatcccatc tggccggaac tggaagcagt
    25381 gctgaaatct cacggtgcgc ataactacgc catctatctc gacaaagcgc gtaatctgct
    25441 atttgccacg atagagattg aatctgaaga gcggtggaat gcggttgcca gtacagatgt
    25501 ttgccaacgt tggtggaaat atatgaccga cgttatgccc gctaatgcgg ataacagccc
    25561 agtcagtagc gaactgcaag aagtgtttta cctgccttaa tgagttgcgg tgccggaaaa
    25621 tatccagcac cgccctttca tcagaagaag tatttaaagc gaacgcgtgc gccgtaacgg
    25681 tcatcactat cgtcgccgtg tatcttatcg ccgtcgctat ccagaatgga gtaatacgtg
    25741 ccgaggtaaa tattaaagtt ctccatatcc atcacattag cgaactgata agacgcatga
    25801 atggtgtgaa tgttgtatgt tccttcatca tcaatccagc aatcgttatc acaaaccacg
    25861 ccactaaatt catcaatctt attatgtgcg tagatataac ccagctcgaa acgtttccac
    25921 agcgcgttaa tcccagcggt gaaatctttc tcattattag catctaaata ggcggtatta
    25981 agattaacca cgatgccatt ttccggatcg gttttcaggc cattccaggt catgctcatg
    26041 ccataaccgg tacgatcgga ctgatcgaca aaattaccct tgctatcggt ataaccataa
    26101 gcattattta ccacgttcgc ttccatcgct gcggaaacgg tgaattcttc cgtcggcgac
    26161 caggcaatta ccggacgcag ataagcaaca tttttctgct gttccatatc gcgtccatga
    26221 taattaccgt cgttatataa agatgttccg tcttccagta acgtgtttaa ttcaaaatac
    26281 cagttatcga gttgtttgct gacgaggaaa ttaccgccag cgttagaacg cccgcggccc
    26341 tctttcatca tatagatata accgctgcca tcgtcataaa gatcgttcgc agtattaccg
    26401 gaatgttcaa caaaggtatc ctgattcagc gggaacatat cgtaggcttc aaaacggcct
    26461 actttgacct tccagtcgtt ttctttgcca aagaagaaaa ccgcatcatc gatatttact
    26521 gaaccgtgca tatcccccag cggttgtgcg gagaacccag cgaaatagcc attatccagt
    26581 tttcgcatac catcaaaacc taacagaata cgaccattga gattccactg ttcattagtg
    26641 ggatcgctat tcacatcagc gttggtcatt gccagcaggc catgattact ttccgcatcc
    26701 atattaaatt ctacgtcacc gtagattttt aaatcgccat accctgaaag caggagatta
    26761 ggcggggtgg cgtttttctt ctctgactcc gtcgttgatt gaccgtttac cggcgtgttg
    26821 ccctgtgcgg ttttaatttc gcggatctcg gcggcttgtt gtgtctgaag ctgtttaacc
    26881 tgcccctctg cttttgctgc ccgcatttct gccgcttcca ggcgttcttc cagcaatgcc
    26941 atgcgttgtt ccagcgttaa atgctgtgtt tttgccatac cggtactaca tgccaataat
    27001 aaaccgatgg ctaaagataa tgtcgatttt ttcattattt acgttccctg aaataaagga
    27061 gaccctacgc tttccaaaag gaatacgtat atagagaaat ataaggtgtg cactttcgaa
    27121 tttttaatcc aacttcagca ggccgatgga tagtatcccg ccatataata tgagtaaatc
    27181 cgctgatttc ctggaacgtg aggaatataa caatagtgcc gtttttaact aaatatcgta
    27241 aaaaatatta gatatgattc acgctgactc ataagccaca aatataaata acgcttaaac
    27301 aaattatctg agatatttta acattcacaa aatcattaaa taatgatact ttattcacaa
    27361 aatttatcct tcactctgct ttatattgtt acgacttcgt gacatatccg tgctcatcac
    27421 aaacccgccc cgcgattaca attaactgaa atatttcatc gaccatttcg ttgtaatcgc
    27481 ataacgctcg tggaaatgag tcttcggcca tccagcttga gtttgcgtcc tgaagttgct
    27541 ctttatccca gtcagagtac gggactttat ctctgatgcg gcgaacgtat tttttagcct
    27601 cgagttgata ctggcctgcc gaattaataa acgagaaatt catgccgctt ttatccgctt
    27661 ctttggcttg cgccaccagc gtttccagtt cagacacttt cttcattgcc gcttctgcat
    27721 caaacttatc ttgcgatatc aggttattaa tttgtttcgc tgaaatcatg actgccagag
    27781 agtagtagtg gaatgttttc cctttcgcaa acgcatcgtc tttataattt tcctgggtgt
    27841 aatatttatc catttcgtta atggtattac ccaacgctac ggcagcatca atataactca
    27901 ccgccacgcc atcaataggt tgcagcgcag gcattaacgc cactacacgc gttttgttta
    27961 taaaaaaata acaaaattca cgaaatgaat aagtcctgta atttaatata tatcgcagga
    28021 cttattttaa ttaattgctc aaacgctgcg gatgggtata aaccgtcgcc cgacccggtt
    28081 tacaaaagcc caccagcgtc agattacagc gctcagccac ttctacagca agtgtggtcg
    28141 cggcagacac cgcaaacaaa atctctacgc cgcacatcgc cgacttttgc accatttcat
    28201 aactggcacg gctggaaacc agcaccgcac cttgctgcca gctttcccct tcttgtgatc
    28261 ggcggcctaa cagtttgtcc agcgccacat ggcgacccac gtcttcatgc ccgccgacca
    28321 gttcgccaga tggcaacatc catgcagcgg cgtgagtaca accagtcagt tgccccactg
    28381 gctggaaatc attgagatga cgtaatgcat catccagttt gttgagatca aacgtctggg
    28441 tgaacggcag cggctgcacc ggtttaccga tatcgttaag ctgttctaca ccgcatacgc
    28501 cacagcccgt acgtcccgcc agcgcccggc gacgttcctt caaccccata aagcggcggc
    28561 tggaaagctc aatttgtact tccaggccat tacaggaagg aacgacatcc atgccgaaga
    28621 tatcgcgcgg gctttcgata attccttcgg aaagcgaaaa accgagcgca aagtactcaa
    28681 ggtctttggg cgacgccatc atcaccacgt gcgaaatgcc gttgtagacc agcgcaacgg
    28741 gaacttcttc cgccacctca tctaaccgtg ggtgttgtaa atcatcacgc cgccataact
    28801 caatttgacg aacacctgtg atatttgtca cattctcgat ttctttttgc tgtgtttttt
    28861 tcactttcta ttaaccagat aagaacacac acaccaacat tatggtattc tgttacaaac
    28921 ccttcctgga tggagggaaa ttgagccaat tctggacctt tgcggcccct tccgcaaaga
    28981 aaaataactc ccactccctg cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc
    29041 gaaacaagga gcaatccatg caggtcagca gaaggcagtt ctttaagatc tgcgctggcg
    29101 gtatggcagg caccacggca gcggcactgg gttttgcacc cagcgtagca ctcgcggaaa
    29161 cccggcagta taaactgctg cgcacccgcg aaacccgtaa tacctgcacc tattgttccg
    29221 taggctgtgg gctgttgatg tacagcctcg gtgacggagc aaaaaacgcc aaagcatcta
    29281 tcttccatat cgaaggtgac ccggatcacc cggtcaaccg cggagcactt tgtccgaaag
    29341 gcgctggcct ggtggatttc atccactccg aaagccgtct gaagtttccg gaataccgtg
    29401 cgccaggttc tgataaatgg cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca
    29461 aactgatgaa agaagaccgc gatgcaaact acattgcgca aaacgccgaa ggcgtgactg
    29521 ttaaccgctg gctctccacc gggatgctgt gtgcttccgc gtcgagcaac gaaaccggct
    29581 atttaacgca aaaattctcc cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg
    29641 tctgacacgg accaacggta gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca
    29701 accactgggt cgacatcaag aatgccaacc tcgtcgtggt gatgggcggt aacgccgctg
    29761 aagctcaccc ggtcgggttc cgctgggcga tggaagccaa aattcacaac ggcgcgaagc
    29821 tgattgtgat cgatcctcgc tttacgcgta cagcggcggt ggctgactac tatgccccta
    29881 tccgttccgg tactgacatt gctttcctgt caggcgtatt gctgtacctg ctgaacaatg
    29941 aaaaattcaa ccgcgaatac accgaagcct ataccaacgc tagcctgatc gtgcgtgagg
    30001 attacggctt tgaagatggc ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata
    30061 agtccacctg gacttacgaa ctggacgaaa acggcttcgc caaacgcgat actaccctgc
    30121 aacatccacg ttgcgtgtgg aacttgctga aacagcacgt ttcccgctac acgccagatg
    30181 tggttgaaaa catctgcggt acgccaaaag acgcattcct gaaagtctgc gaatacatcg
    30241 cagagaccag tgctcacgat aaaactgcct cgttccttta cgccctcggc tggacgcaac
    30301 actccatcgg tgcgcagaac atccgtacga tggcgatgat ccagttgctg ctcggcaata
    30361 tggggatggc tggcggcggc gttaacgccc tgcgcggtca ctccaatatt caggggctga
    30421 cggacctggg gctgctgtcg cagagtctgc caggttacat gacgctgcca agcgagaagc
    30481 agaccgatct gcaaacttac cttaccgcca acacgccgaa accactgctg gaaggccagg
    30541 ttaactactg gggcaactac ccgaaattct tcgtctctat gatgaaggcc ttctttggtg
    30601 ataaagcgac ggcggaaaat agctggggct ttgactggtt gccgaagtgg gataaaggct
    30661 acgacgtcct gcaatacttc gagatgatga aagagggcaa ggtcaatggc tatatctgcc
    30721 agggctttaa ccctgttgcc tcattcccga acaaaaacaa agtgatcggc tgtctgtcga
    30781 agctgaaatt cctcgtgact atcgacccgc tgaacactga aacctctaac ttctggcaga
    30841 accacggtga actgaacgaa gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc
    30901 catcgacctg cttcgcggaa gagaacggtt caatcgttaa ctctggtcgc tggttgcagt
    30961 ggcactggaa aggtgcggac gccccgggga ttgcgctgac tgatggcgag atcctctccg
    31021 gtatcttcct gcgcttgcgc aagatgtatg ccgaacaggg tggcgcgaac ccggaccagg
    31081 tgctgaacat gacctggaac tacgccatcc cgcatgagcc gaagtcggaa gaagtggcga
    31141 tggagagcaa cggtaaggcg ctggccgata ttaccgatcc ggcaaccggt gcggttattg
    31201 tcaagaaagg ccaacaactt agctcgttcg ctcaactgcg cgatgacggt acaacctcct
    31261 gtggctgctg gattttcgcc ggtagctgga cgccagaagg caaccagatg gcgcgtcgtg
    31321 ataacgccga tccgtctggc ctcggtaaca cgctgggctg ggcatgggca tggccgctta
    31381 accgccgcat tctgtataac cgcgcctccg cagatccgca gggtaacccg tgggatccga
    31441 agcgtcagtt gctgaaatgg gacggcacta agtggaccgg ctgggatatt ccggactaca
    31501 gcgcagcgcc tccgggcagt ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc
    31561 gtctgtttgc cctcgataag atggcggaag gtccgttccc ggaacactac gagccgtttg
    31621 aaacgccgct gggaactaac ccgctgcatc caaacgttat ctcgaatccg gcggcgcgta
    31681 tctttaaaga cgatgccgaa gcattgggga aagccgataa gttcccgtat gtcggaacca
    31741 cctatcgtct gaccgagcac ttccactact ggaccaaaca cgcgctgttg aacgcgattt
    31801 tgcaaccaga gcagtttgtg gaaatcgggg agtcgctggc gaataaactt ggcattgccc
    31861 agggcgatac tgtgaaagtc tcctccaacc gtggctatat caaagccaaa gcggtagtga
    31921 ccaaacgtat tcgcacgctg aaagcaaacg gcaaagatat cgataccatc ggtattccga
    31981 ttcactgggg ctatgaaggt gttgcgaaaa aaggctttat tgccaatacg ttgacgccat
    32041 tcgtcggtga tgcgaacacg cagacgccgg agtttaagtc cttccttgtg aatgtggaaa
    32101 aggtgtaacg gagacgacat atggcttatc aatcgcaaga tatcattcgt cgttccgcga
    32161 ctaacggtct gacccccgcg cctcaggcgc gggacttcca ggaagaagtg gcgaaactca
    32221 tcgacgttac cacctgtatc ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg
    32281 acattcgcga taccgtcggc aataacattg gggtgtatga caaccccaat gatttaagtg
    32341 ccaaatcgtg gacggtgatg cgattctcgg aagtggagca gaacgacaaa ctggaatggc
    32401 tgatccgcaa agacggctgt atgcactgtt ccgatccagg ctgcctgaaa gcatgcccgg
    32461 cggaaggggc aattattcag tatgccaatg gtatcgtcga cttccagtcc gagcagtgca
    32521 tcggctgcgg ttattgcatc gcagggtgcc cgttcaacgt gccacgcctc aacccggaag
    32581 acaaccgcgt ctacaaatgt acgttgtgcg ttgaccgcgt ggtggttggg caagaaccgg
    32641 cctgcgtgaa gacctgccca acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa
    32701 cgctggcgag cgagcgcgtg gcggagctga aaacccgcgg ttacgacaat gcgggtctgt
    32761 acgatccggc aggcgtcggt ggtacacacg ttatgtacgt actgcaccat gctgacaagc
    32821 caaatctgta tcatggcttg ccggagaacc cggaaatcag cgaaaccgtg aaattctgga
    32881 aaggcatctg gaaaccactc gcagctgttg gctttgcggc taccttcgca gccagtatct
    32941 tccactacgt gggtgtcggt ccgaaccgtg cggatgagga agagaataat ctgcacgaag
    33001 agaaagacga ggagcgcaaa tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga
    33061 acgtatcaac cactggatca ccgccttctg cttcatcctg gcggcggtga gcgggctggg
    33121 ctttttgttc ccgtccttca actggttgat gcaaatcatg ggcacaccgc agctggcgcg
    33181 aattctgcat ccgtttgtcg gcgtggttat gtttgcctcg ttcatcatca tgtttttccg
    33241 ttactggcat cacaacctaa tcaatcggga tgatatcttt tgggcgaaga atattcgtaa
    33301 gatcgtcgtc aacgaggaag taggtgacac cgggcgttat aacttcggtc agaaatgcgt
    33361 tttctgggcg gcgattattt tcctggtcct gttgctggtg agcggcgtga ttatctggcg
    33421 tccttatttt gcgcctgctt tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc
    33481 atttgccgca gtggcgttaa ttgtggttat catggtgcat atctacgccg ccctttgggt
    33541 gaaaggcacg attaccgcca tggtggaagg atgggtgacc agcgcatggg caaagaaaca
    33601 tcacccgcgc tggtaccgtg aggtccgcaa gacaacggaa aagaaagctg aatgagtatt
    33661 cgcataatcc cgcaagatga gctgggttcg agcgagaaac gtacggcgga tatgattccg
    33721 ccgttattgt tccctcggct caagaattta tacaaccgcc gcgccgagcg tctgcgcgag
    33781 ctggcagaac ataatccgct gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc
    33841 caggaagtgg tgctgtacga ccatccgctg gagatggatc tgaccacgcg cattaaagaa
    33901 gccagcgcac aaggcaagcc cccgctggat attcacgttc tgccgcgtga taagcactgg
    33961 caaaagctgc tgatggcgct gattgctgag ctgaaacctg aaatgagcgg cccggcgctg
    34021 gcagtgattg agaatctgga gaaggcatcg actcaggagc tggaagatat ggccagcgca
    34081 ctgtttgcct ctgatttctc gtccgtcagc agcgataaag cgccgtttat ctgggctgcg
    34141 ctgtcgctct actgggcgca gatggctaat ctgatccccg gcaaagcccg cgctgaatac
    34201 ggcgaacaac gtcaatattg cccggtatgt ggctctatgc cggtgtccag catggtgcaa
    34261 attggcacca ctcagggtct gcgttacctg cactgcaacc tgtgtgaaac tgaatggcac
    34321 gtagtgcgcg taaaatgcag caactgtgaa cagagcggca aactgcatta ctggtcgctg
    34381 gatgacgaac aggccgcgat taaagccgaa agctgcgatg actgcggcac ttacctgaag
    34441 attctttatc aggaaaaaga accgaaagtt gaagccgtgg cagatgacct cgcctctctg
    34501 gtactggacg cgcgaatgga gcaagaaggc tatgcccgta gttccatcaa cccgttcctg
    34561 tttccgggtg aaggggagta atctctgacg ttttccgggt ggcgcaggtc gcccggatta
    34621 cagttcatag ggtgtgacga cactatctct cgtattccgc gttacctcct caagctatgc
    34681 cgccagtgcc tgtttggcct caattttcac gctgagaaaa tcgataagga tatcgatatc
    34741 aaatagccaa atctttttgc cattaccatt tctcgcgcag cttacgcagg tattcgacac
    34801 catcttcctc acacccttgc cagatgccag gaacactggt tcttgctgtt tgcgattgat
    34861 ttaccaggta ttgatctacc gcttcccgta ataggtctgc acgcggaaga ttacgctgca
    34921 cctcaagatc atcaagttgc ttaatcacct cattcgataa atcgagtaaa attctgctca
    34981 tatccatacc tgccagaggg ttcataaata tctcgccaat atcattttga atctatggag
    35041 agaaaagtac ccttgtcgaa tctttaaaga aagcgcattt acgcatcact ttttattttt
    35101 gcgaggcgtt ttccagatac gatgtaacag ttgcagttat tacgaaaacc agtaaaaaat
    35161 ggaagtgaaa acagtcgtgt gaaaatttct actctttctg aatttactct ataaagttgc
    35221 ctttctcaca tccaatagct taatgtcatc taccaagaaa aaacgataag tcgtcaggga
    35281 acggaacaga tataatgaat aaaaacgaat caatttctta caccgaaatg caaaaatttt
    35341 ttgccagaga aattaaggaa gtaggcggta ggcccatcac ttctaagaat ttagagttaa
    35401 gtctgctacc cattcacgcc agggaaaacc tttctgcgcc agaaatgccg tggtataccg
    35461 aagttaatga tatctatatg gagttcagac gctcaccatt ctggatggtt gccggttatg
    35521 tattcctggg atttatgctt tcactacttt ccatctcgat attatctatt gttgcattct
    35581 cattttatga gatgggcttt aatagtgact tgttatttct gtatctactt gcatttgtgc
    35641 ttggctgttt tatattacct ggcgctgcaa aaattgcttt tttcacgcca cattatcttc
    35701 ctataagatt taacagaaaa acacaaaaaa tttatgtttc agatttaatt attgaaggca
    35761 atatcttctt tggaaaaata aaactcactt ttcatgaata tgactggaaa gatgttgaag
    35821 gttgggaggt gagtagacat ttcagtaaaa gtatgcctta caatggatta catctggtag
    35881 ttaatagaga aaatgatata aatgcactgc gacagacacg cgtttataca acccgtgcac
    35941 cgtggagtgc acaagaaatg gcaagacaaa agaactatat cccccaaata tggctttatt
    36001 gccataatta tatggcttat aaaactgtgt ctggtgcaaa gaacaaacca caaccattta
    36061 tttttttaag aaacaattgg ctatttaaat ggccaaaaga aatggataaa cgttccagaa
    36121 gctcaaagat actgtctgaa taagcataat aatgaaggat ttcgactatg attaacaaac
    36181 ttactcttcc ttggccggta ttatttttag aattttttgt gcgagaagtt gcctttaagg
    36241 gtaagggaat tgttcgttct acattaccag cagatgccca tttttatacc ggtgggtcaa
    36301 aagccctgcg ttatcaccta caaccaggaa accaatggcc aaactatata tggcatgatg
    36361 atataggtga tattgtaccg tttcctctac cctcacttcc gcttacattt accgctaacc
    36421 gacccacatt taattccaga gaattagcgg ataaatggat gaaggataat attcataccg
    36481 ctgtcatcat ggcgggggaa tttctgtctg ctaatgaatg gattcgccgc aaaaaccagc
    36541 ttaaaactag tgtacaccct gcgtcagttg cttctttcgc accctatggc tttaacatta
    36601 accagtgtga tgacgatggc aatctttgga ctaatttccc acgtccccac atcaaattaa
    36661 aaatcgcacc aggaaaaaca gcttgttttc tctgccgccg ggaagagctt ttatttggtt
    36721 atgatgaagc acataatcac taccagccga atcttaatct ggttttattt gtgaaaaatc
    36781 atgggttgca taaggatgtt tacctaaaat ctcatgacga taaaagtgaa tacgatagca
    36841 gaatattatg taacgaaaaa tttaccactt atgatttccg taaaaaccgg tccagataca
    36901 gcttttgata gtttattatc ctggatgata tcaggagcga tatctataaa gtttatgcat
    36961 tccctgtgat caatttttcc tgttagtgag cacaaattca atctgattga tgccagaata
    37021 aaggcatctg gcatgttgat tttatgttcc agaatgacaa aacattgctg ttaataagca
    37081 cgactctttt tgtcattcct catgccactt accctgttct tcaacaccaa ctacctcacc
    37141 cctttgcctg tcactttttg ccttacgcac cggaaattgc tgacgctcca gatattgctc
    37201 taccgcttcg cgtaaaattg cagagcgcga aaccttacgc cagccccgaa gcttgttcag
    37261 ccgtttaatt gcctcttctg ataaatgaag aaaaaccgta ttcatcgcca ttgccaccac
    37321 cttacatata ttactcatta atgtatgtgc gaaaattacg ccgaaaaatc atttaaaaaa
    37381 agcggatttt tgcatgtcgt tttgtttttg cgagactgca tccaaagata atgtaacagt
    37441 tgcagttatt tcaattgtta ctcaaaaatg gtttattact tttaattaag cgggggcaca
    37501 tctacagaaa agaaacaaca aataataagg atgaattatg gatgctataa atattttata
    37561 ttcaagaata tttttttgat tgggtcttca tttgcttcat caggatggtg tacccgaaaa
    37621 agtcagcagc tttaccgcac caggggttaa ggtgaaatct accattcata acgaccggtc
    37681 agtgccgcac caaccggtct ttaattgtta gtagtagtaa atttgtggat aacagtccgg
    37741 gaaccagacg attccttccg gatcatcccc ctgcattacc cgatgacatt ggcgtcgatc
    37801 atctgcgcga cggcgaagcg tagtatagag ccgcttccat tcatgatcgc ccggtgtact
    37861 gtagtttacc caacaatcca ttgaagggtg acgccattta cctgtaagtg ttgggatccc
    37921 tgaatgctta tgcggcttcg ctttaccatt acgatcccta tctttacgcg tattaaacgt
    37981 ggacattttt agtctcctgt taagagttct aaaaatgtgc ttccttaaaa tagttcatca
    38041 ttttccctta attcgcatag cgtgcttttg cacgaatttc cgatacgcgg ggaccattat
    38101 gatagcgtcg atgcccacac atccaacaag aacaaataca gggagtacga aagcaaatac
    38161 ctgatgcttt tttgcttcca tcgcctgcag tgcgataacg acagcggatt ttttttaatc
    38221 gcaccatatg atggcgacgt tcagcacgat tacgggtcat agcaaaactc ctgaacaggc
    38281 ggcactatgc cgctttcata ggagttctga atgctggtgc tttctgtatg gcataaccat
    38341 gcctctaacg gttaagtgct tgcagcacat gttgcggatc attggcaatc gctcgcagca
    38401 aagcctttgc aggcccggtc ggtgaacgtc gcccctgctc ccagttacgc aacgtgtcta
    38461 cgcttacgga aataagatcg gcaaatttag attgtgacaa ccctgaagcg cggcgtattt
    38521 ctttgatttt catagcatca acttcaaaag tccgtgaagg ctgtcgcgtt ccttcaataa
    38581 tttcattcat ctgggccatg ctgtgagtca accgctcaaa aagtgcctta tccatttacc
    38641 acctcgtatt taacaaccgg agcattgctt tttcctgggg agacagatca tctttaatac
    38701 ccttacgata aataagcagt aatctgatct cataatgttt aacctgatga aagtaaataa
    38761 ccctgacacc agcacgtttc ccctttcctc ctgagaccca tcgaaccttt cttaacccac
    38821 cagtttcagg tattacctca ccatagtcag gattcaaggc caggaaaaac tgaaaacgac
    38881 tgaattcatc gtcgttcagc agtttttgaa catcttcagt aaaaatttcc gtttcgatga
    38941 agagcataat tatacgccat tggcttagag caacaatcct ttttgtacgt cattgacgta
    39001 ccattgaaac cactagctta ctgatagtgc tccagcaaaa ccacgtctct gccgggtttg
    39061 tctttacatt tgcgaggcgc tttccagatt tttcacgccc gtgaatttcc ctgtatccat
    39121 ccagcaaaaa cgggtgtata aaactgtaca ttcatacagc aaaatgagtt ctactatggc
    39181 actggaaaaa ggaattgcag agttagtcga agagtttatc gctgcgggtc gcccctcatc
    39241 gcgccaacaa agtattacgc agcgcagaga gggctatatc gccagcgctg tccttgcggg
    39301 tgagacagaa acgcgggtag atattcagac tatcgaactt gaagggatga ccttacgaat
    39361 agtgtcaccg ctgaatgccc cgacattact cccgacgata atttattact acggtggctg
    39421 ttttgttagc ggcgggttcg caactcacga caaccagctg cgccagttag cctattacgg
    39481 gcaatgtcgg gttattgccg tccagtatcg gctggcaccg gaacatactt tccccgccgc
    39541 ccatgatgat gcacaacgag gtgcagaatt agtccggcaa cacgccgaac gactcggcgt
    39601 tgataaacaa cgaataactc ttgccggtga cagtgcggga gggcatctgg cgctggtaac
    39661 tgctttgcga ttgaagcgtg ctggcgagtg gcaaccagca caactcatct taatttatcc
    39721 tatgcttgat gcgactgcgc atttcgaaag ctacatacgc aatggtcacg attacatcat
    39781 cacacgcgac actttactga gcggctttga gatgtacctg ccaggcattg aacgcaggca
    39841 tcctgaagcc agccctattt ggcgcaatga tttcaatggc ttgccacctg tacatatcat
    39901 taccgctgaa tacgacccac tttgcgatga aggtgaagcg ttgtatcacc gcatgacagg
    39961 gcaaggagtg caatgcacct gccaacgcta ccttggcgtt atccacggat tttttcagtt
    40021 aggtggtatc agtgaggctg cgcgatcggc gttacgggat gttgcctgga gagcgggaag
    40081 gtaactccgg accagggccc agaaaaggtt ttcagattga aaaccttttc agtcaagtaa
    40141 aacgccccta ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca
    40201 gaacgatata cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttctactt
    40261 gcatctgcac ccgtgctttt cgtccgcgcg ccagacggtc gagatcgcca cttaaggcac
    40321 caaggtcggc taccgcatgg ggttttccgc taatcggctt gctgtagcgg atatgcgcat
    40381 ccgccagaat aatcgttccg ccgaggtggc gttcgcgcag catcagccag ataagccccc
    40441 aaccggtgag cgtagccagc gagaataaac tcccggcaaa cagcgtgtga tgcggattct
    40501 gattgccggt ttccggcatg gtggtgataa atttttgccc ggtatattgc tgaatgcgca
    40561 cacccatttt ttcgcttaac gggatatgtt cataccacgc ttgctggagc tgggcgcacc
    40621 agtcgccgcg atgaagaata tcatccagag tggcgacggg tttaatcatc agaaaatggc
    40681 gaatcggcgt ggtggttggc gtggtgattt caccctgatt aacaaacccc agcttggcga
    40741 aaaactccac cgcgtcttca cgggcgctac aggtcacgcg cttaacgcct tcctgacgtg
    40801 ccaccgactc cagggtcatc gccatcagcg tgcctaaccc tttgtcctgc acgtcgggat
    40861 gaacggccat aaagcgaatg gacgcttcat tgtcggcatt aatatacagt cggcctaccg
    40921 ctaccagatt accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt
    40981 cgcgttccga accttttggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat
    41041 agtaacgctc taattcttct tctgtttgtg gaacccgaag gtgatacata gcgatactct
    41101 ctcttgtttc ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg
    41161 gccatcattg accagcgata cctgcatatc cgcagcgaag cgtcctgttt gcgtgttcat
    41221 atcttgctga cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga
    41281 tgcgcctttg gagaaacttg ggcgcatccc ccgttcggta tctgcggcaa gggtaaactg
    41341 ggaaaccacc agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc
    41401 atcgctaaag atgcggtagc cgagtacacg ctcgcacaga cggtttgctt tctgttcgtc
    41461 gtcatccttt tcgacaccca ataacaccaa aagtcccgcg ccaatttcac ccgtcacttc
    41521 tccctccacg gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg
    41581 tcttcttctt gttcagcagc ttgttttaat ttgcggtatt ccccgagagt gacagtaatt
    41641 tccgcgccaa gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc
    41701 accgccagca cgccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa
    41761 cctttctttc ctgcttcgaa caggagcgcg gcgacaaacg cgccgacaat cgcgtcgcgg
    41821 ttaggtacgc gaatggtagg aacgatgctg tacagcaacc aaaaggagat ccacgataac
    41881 agcagcggaa aaatacgcag cacgttgtcg atgacagtat tgagatcgct cgcccagcgc
    41941 agggaaagca gataggagct gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt
    42001 aaaatcatcc agtacacggc aaacgagtaa attttgggtc gcgctcgttt actgcgccag
    42061 atggtattca acgcgctatc gatggagtac atcaacaata acgccgtgac gatcagcccg
    42121 cacgccccga cggcggtcat tttgttggaa ttggcgacaa actgttcgat atatcgctgg
    42181 ataacatcgc cagtagcagg cagaaagttg gcaaaaataa agtgacgcaa ctgaatgctg
    42241 acgtcggaaa acatgggaaa agcggcgaaa agcgcaaaaa caacggcaac cagcggcact
    42301 aatgagagca acgacacata ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca
    42361 atgcgttgcc agagtagttt tagccaggcc catagtggac gggtacgatg cctggctttg
    42421 tcctgaatgg tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt
    42481 tcaccagaat actagtaatg cctagccgat tagctccttc tatattatcg gcgttatcgt
    42541 cgaaaaagac cgtatcgtct ggtgaaaaac cttctgcccg caaaacatgc tgataaattc
    42601 gtgcttcagg tttgcgcata cccaagtctt gcgacagata gatatggtca gcagcatcac
    42661 ggatttccgg gtattcctcc ggccagaagg tagtatgcag gcggttagta ttggaaagca
    42721 ccaccacgcg atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccggtcgta
    42781 gcgcaacaaa taccgcctgc caaccgtggg agaactgctc atagcttagc ggcagggcca
    42841 tctcatgaca cagtgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat
    42901 gaaacgcctc gcccatgtgg aaactcttct taagcgtagc cagtggaaca cgcgttaaat
    42961 cgctccaggc ccccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa
    43021 agatatagag catttttgcc tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga
    43081 tgggtttggc tatgtggcaa taagggtaat cttgtgatgc tgattgcgaa aaagcagagt
    43141 aatttcactg gctttttcgc aatagatgaa tggttaagat tcaggtgtaa attgccaacg
    43201 agtttcgatg accagcggct tatctggtgt cacaaattcc ggcgttagat ttattgcgtt
    43261 aggtgcctga gtcagcgggt taatacaggt ggcatcgggc tgtttatcaa ataccaccag
    43321 actgtttgca gaagaggtca tggtcatcgc gagtttacca ggccacagta acttaacctt
    43381 caccccgtca taaaaaccaa aacagtcatc ccagggacca acttgaggag agatacgatt
    43441 tccagttggc agttcatcac tacccgcttc ttcttgccag tccgcatcaa aaagcacctg
    43501 caacgattct gtattttgcg gagtcagctt tttcgcaaac cacggatgcc agcctgcgga
    43561 ggccgggaat gtatcggcat atgaatggac ttccagttgc aaaaccagcg catcgttctc
    43621 cagcaaaaaa gtctgaatca cttccccttg ccagggccac ggtgaagtca ggggcatacg
    43681 taaagtcagg gaatctgctg ttttgtcgat gatttcccac gttgagtaac aagccatacc
    43741 atgcaacgca tgtggtggtt tattggcggg taaagaataa cattgcccac cagcattcag
    43801 cgtcgcattc cccagtcgcc ctgcccacgg caccattggg aaacagccat actgaaaagc
    43861 tctgcgctgc ggctgccatt ggcgtaatac ttcagaacca aatgccttta acgaggttat
    43921 cctggcacca tcatgcggat aaatctgcag gtgaatattg cctttttcca gcgtcactaa
    43981 ctctcctaca ggcccaggcc atggcgcgtc aaacgcctgt tgtagcgcca ggtaatgctc
    44041 gttttttcgt tcagttttca tgagattgtt ccctgatgta gcggtaagaa gcggtttctt
    44101 gttcatcggt tggctcaatc agccagacgc aaacaaagga aagaactgag acagcaaata
    44161 aaaacagagc gacataccac ggttttcctc cccctagcgc cagcaaactt gaggcgataa
    44221 gggggcttag tccgccaaga atcgcggaaa attgataagc aaaagaaagg ccggtatatc
    44281 gtactttggt accgaacatg cgggtgaaaa gtgttggctg cacggcaaac atcatcgtcg
    44341 gacctaagtt gtagccaagg atcatactgc accagatgat gagcgtactt ttgctatcca
    44401 gtaataagaa gaaaggaaac gcgaacagga cacaaaaccc tgctcccgac aaatacagac
    44461 ttttttgacc tatacgatct gataaccatc caaccaatgg acaactgaaa ataccgatcg
    44521 ccgaggcaat caacataccc gtcaggggaa tatctcgcga caatgctaat tggctggaaa
    44581 tatagacaat accaaaggcg ttaataatat tagaggagac actttccgcc agcctcgcac
    44641 ccagtgccaa taaaatattt cgtggatgtt ttttgaataa ttcaatcaac ggaggaacgg
    44701 acttttcttt attattcgtc gttttttgct tttgaaaatc cagcgtttct tcggtatgca
    44761 ggcggataaa cagcccaacg atcaacatca ccgcagaaag ccaaaacgga atgcgccatc
    44821 cccaggagag aaactgctcc gaagtaagaa aatggttaca gagagcaaaa ataccggttg
    44881 ccagcataat acccacagac gcagccgttt gcgggagtga acctaaaaat ccgcgtcggg
    44941 attcgggcgc tgattctatg gtcatcaatg ccgctccgcc gtactcgcca ccaagaccaa
    45001 agccctgaat taaacgtagc accattaata tgagcggtgc ccagatccca atttcctgat
    45061 acgtcggaat aaaaccaatc aaaaatgtgg agcaaccgac aatagccaac gtccagataa
    45121 gcgttatttt tcgccctatt ttatcgccaa aatgaccaaa gacgatgcca cctaaaggac
    45181 gagtcagaaa accgacacca aaggtcgcaa aggaagctat aagacctata aaaggatcgc
    45241 tgtctggata aaacagcggt gcaaatacca gtcctgctgc cgtgccaaaa ataaagaagt
    45301 cataccattc cattaacgcg cctgcgaaac tgcccataac gacacgtctc atggctttac
    45361 cggaaagccc ttcacgcggt gcgggtaact tttcagacat aattttcctc tcctggtagg
    45421 cgagaaatct gggatacgaa ccacta
//
LOCUS       DAOFSV010000027        45303 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.027, whole genome shotgun sequence.
ACCESSION   DAOFSV010000027 DAOFSV010000000
VERSION     DAOFSV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.027"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1960)
                     /gene="aaeB"
                     /locus_tag="QBH27_001565"
     CDS             complement(<1..1960)
                     /gene="aaeB"
                     /locus_tag="QBH27_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDQ7013890.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPCMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALT"
     gene            complement(1966..2898)
                     /gene="aaeA"
                     /locus_tag="QBH27_001566"
     CDS             complement(1966..2898)
                     /gene="aaeA"
                     /locus_tag="QBH27_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDQ7013891.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(2906..3109)
                     /locus_tag="QBH27_001567"
     CDS             complement(2906..3109)
                     /locus_tag="QBH27_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDQ7013892.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            3292..4221
                     /gene="aaeR"
                     /locus_tag="QBH27_001568"
     CDS             3292..4221
                     /gene="aaeR"
                     /locus_tag="QBH27_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDQ7013893.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(4343..5788)
                     /gene="tldD"
                     /locus_tag="QBH27_001569"
     CDS             complement(4343..5788)
                     /gene="tldD"
                     /locus_tag="QBH27_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDQ7013894.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVNNLTVGGTA"
     gene            complement(5944..9744)
                     /gene="yhdP"
                     /locus_tag="QBH27_001570"
     CDS             complement(5944..9744)
                     /gene="yhdP"
                     /locus_tag="QBH27_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDQ7013895.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTSITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLSVNVKVDGNLNSFELTGQAGADNYFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(9812..11281)
                     /gene="rng"
                     /locus_tag="QBH27_001571"
     CDS             complement(9812..11281)
                     /gene="rng"
                     /locus_tag="QBH27_001571"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDQ7013896.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(11271..11864)
                     /gene="yhdE"
                     /locus_tag="QBH27_001572"
     CDS             complement(11271..11864)
                     /gene="yhdE"
                     /locus_tag="QBH27_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDQ7013897.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(11873..12361)
                     /gene="mreD"
                     /locus_tag="QBH27_001573"
     CDS             complement(11873..12361)
                     /gene="mreD"
                     /locus_tag="QBH27_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDQ7013898.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(12361..13464)
                     /gene="mreC"
                     /locus_tag="QBH27_001574"
     CDS             complement(12361..13464)
                     /gene="mreC"
                     /locus_tag="QBH27_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDQ7013899.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(13530..14573)
                     /gene="mreB"
                     /locus_tag="QBH27_001575"
     CDS             complement(13530..14573)
                     /gene="mreB"
                     /locus_tag="QBH27_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDQ7013900.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(14878..16818)
                     /gene="csrD"
                     /locus_tag="QBH27_001576"
     CDS             complement(14878..16818)
                     /gene="csrD"
                     /locus_tag="QBH27_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDQ7013901.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     LVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16970..17944
                     /locus_tag="QBH27_001577"
     CDS             16970..17944
                     /locus_tag="QBH27_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ7013902.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVTDLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18921..19391
                     /gene="accB"
                     /locus_tag="QBH27_001578"
                     /gene_synonym="fabE"
     CDS             18921..19391
                     /gene="accB"
                     /locus_tag="QBH27_001578"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDQ7013903.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            19402..20751
                     /gene="accC"
                     /locus_tag="QBH27_001579"
     CDS             19402..20751
                     /gene="accC"
                     /locus_tag="QBH27_001579"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDQ7013904.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(20843..21889)
                     /locus_tag="QBH27_001580"
     CDS             complement(20843..21889)
                     /locus_tag="QBH27_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDQ7013905.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(21886..22848)
                     /locus_tag="QBH27_001581"
     CDS             complement(21886..22848)
                     /locus_tag="QBH27_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ7013906.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(22870..23859)
                     /locus_tag="QBH27_001582"
     CDS             complement(22870..23859)
                     /locus_tag="QBH27_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013907.1"
                     /translation="MTEKTLASPKPSGIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(23860..25359)
                     /locus_tag="QBH27_001583"
     CDS             complement(23860..25359)
                     /locus_tag="QBH27_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013908.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(25420..26310)
                     /locus_tag="QBH27_001584"
     CDS             complement(25420..26310)
                     /locus_tag="QBH27_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDQ7013909.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLSGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(26346..27200)
                     /locus_tag="QBH27_001585"
     CDS             complement(26346..27200)
                     /locus_tag="QBH27_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HDQ7013910.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     TEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            27541..28371
                     /locus_tag="QBH27_001586"
     CDS             27541..28371
                     /locus_tag="QBH27_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7013911.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            28403..29338
                     /locus_tag="QBH27_001587"
     CDS             28403..29338
                     /locus_tag="QBH27_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ7013912.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            29431..29673
                     /locus_tag="QBH27_001588"
     CDS             29431..29673
                     /locus_tag="QBH27_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDQ7013913.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVRFIYRDIPLEDDDAA"
     gene            29663..31114
                     /gene="panF"
                     /locus_tag="QBH27_001589"
     CDS             29663..31114
                     /gene="panF"
                     /locus_tag="QBH27_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDQ7013914.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            31126..32007
                     /gene="prmA"
                     /locus_tag="QBH27_001590"
     CDS             31126..32007
                     /gene="prmA"
                     /locus_tag="QBH27_001590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDQ7013915.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            32336..33301
                     /gene="dusB"
                     /locus_tag="QBH27_001591"
     CDS             32336..33301
                     /gene="dusB"
                     /locus_tag="QBH27_001591"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDQ7013916.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            33327..33623
                     /gene="fis"
                     /locus_tag="QBH27_001592"
     CDS             33327..33623
                     /gene="fis"
                     /locus_tag="QBH27_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDQ7013917.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            33708..34592
                     /gene="yhdJ"
                     /locus_tag="QBH27_001593"
     CDS             33708..34592
                     /gene="yhdJ"
                     /locus_tag="QBH27_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ7013918.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            34676..34855
                     /locus_tag="QBH27_001594"
     CDS             34676..34855
                     /locus_tag="QBH27_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDQ7013919.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(34858..35520)
                     /gene="acrS"
                     /locus_tag="QBH27_001595"
     CDS             complement(34858..35520)
                     /gene="acrS"
                     /locus_tag="QBH27_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDQ7013920.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
     gene            35919..37076
                     /gene="acrE"
                     /locus_tag="QBH27_001596"
     CDS             35919..37076
                     /gene="acrE"
                     /locus_tag="QBH27_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDQ7013921.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            37088..40192
                     /gene="acrF"
                     /locus_tag="QBH27_001597"
     CDS             37088..40192
                     /gene="acrF"
                     /locus_tag="QBH27_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDQ7013922.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            40445..40666
                     /locus_tag="QBH27_001598"
     CDS             40445..40666
                     /locus_tag="QBH27_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013923.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            41097..42112
                     /locus_tag="QBH27_001599"
                     /pseudo
     CDS             41097..42112
                     /locus_tag="QBH27_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
     gene            42180..43361
                     /locus_tag="QBH27_001600"
     CDS             42180..43361
                     /locus_tag="QBH27_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDQ7013924.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAAVSIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            43371..44474
                     /locus_tag="QBH27_001601"
     CDS             43371..44474
                     /locus_tag="QBH27_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDQ7013925.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            44482..45240
                     /locus_tag="QBH27_001602"
     CDS             44482..45240
                     /locus_tag="QBH27_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDQ7013926.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT        11221 a        11621 c        11069 g        11392 t
ORIGIN      
        1 cggtcaacgc atgttgatac ttatggagca tacccgtaag ccgatgtact ggttccgtca
       61 cctgcggtgg cgcttgccag atgcgtagct tttcctggta gatttccagt tcttccagca
      121 actggccaaa gtaccgacga cgtttatcat cgctacgggc agatatcaca tggtctgctg
      181 tgcggcgcat ttgtcggtga aacgccgata aatcctcgtt aaccgggatc ggcgcatcac
      241 gcaggcgctg gtgcgcgata atcatcgtca gcgccaggcg aaatttcggc aaatcccctg
      301 ggaacttatt catcagcaaa aacagctgct gataaagtgc cgggaggtga ttctctttac
      361 gacgtgccac attggtagtc atcgcggaaa cagcggcaga aacaaactga ttaagcagta
      421 cacgtccagt cctgtcgcgc gatttatccc gcaccagcaa aataacggtg aacgcgagca
      481 cacagccgac gatttgccct aatgcgctgt cgagaaactg actgaaatgg aaagtcatcg
      541 ggttatccag cacgataatg tttatggtgc tggccaacgc ccccatcgag cccaatctcc
      601 gtttctgtac ttctatgccg aggaaaaatc ccagcactgc caggctaata cacagcagca
      661 acatgctctg ttgggtatta gggataatca ccaaaaagta gagcagccct aacggcagcg
      721 ctgccagcgt cccgtagata aagtcgatcg ccaccatgca tggattcggc aaacgcattg
      781 ccagtgacgt cacgaccgca atcatcacca ttgcaccact gccggaagtc cagcccgtcc
      841 acagccagaa aagcgtgccc agaatgcaag aaagtgtggt tcgccagaag ttaaccattg
      901 catgatgacg ttcggctgac tctactttta cttccggttc gccttgcagg atctcttctt
      961 cggtggcgtt gatttttgtg ttactgataa cgccgcgctt gagaagctga taacgcgttg
     1021 ccgccgcgac ccaactataa atggtaacag gcgtttcccg ttccccggtc caggcgatca
     1081 ctctccgcag gcgtttgagc tgcttgtgga cgtcctgcgc agtttctacc ggcgtgtcaa
     1141 aaaattcgcg gaaagtatca gtgatcaatt ccgggcgcgt attctgaata agataagttt
     1201 cgcaggattg ggtaatcagc gtcagcgata gcgtattgat cgcttttaaa cgtcgattag
     1261 cccgcgccca gcgggaagat tccatattca ggttgctgcg cataccttgt aacgccgtag
     1321 tacgtcgcac cagatcgccc caggctttat cgacaacttc accatcgcca tgcttgatac
     1381 agagttgcat taattgatat tgcgcgacca gcaaactttc cagctctcga tccacttctt
     1441 gtttgatcga tcgcggagaa aagagcaaat ccgccataat tgcacacaca ataccgataa
     1501 cgatctcgct acaacgttcg acggcaaact gcggcgtaag caatggttcc ggctgaatgg
     1561 tgatcacaat gatcagcgcg gtataaccgg ccagccccca cgcatacgag ttttctattc
     1621 gtaccagcga ggatatccag gtacaaaaac cggcccagat acagcacacc agaatcatca
     1681 ataatggtgc tcggatcatc gcaatgatga ttaccagtcc ggcaatacag ccaataaatg
     1741 tgccgatgat gcgcaaaaag ccacgatagc gaatagcgcc agaatacggt tcacctcccg
     1801 cagcaaaggc cggaccggcg gcaacaatcg ccgctgtcag taccgcccag cgtggcgttt
     1861 ccagctggaa gtgaaagcca acaaacagcg ccagtacaat ggcggtcgcc agttttaccg
     1921 caaagcgaat atgttgatta gcaatggaga aaatacccat cgtgattaac caaactcacg
     1981 caggcgatga gccattttac ggaagaacga atcctgactt tcgtcgcgat cttgtttgcc
     2041 agtgactacc accgtagcag tggtgcccgc aggccagatg ttctcttgct ggttgtcgag
     2101 acgaatacgt accggaacac gttgcgccaa acgtacccat tcaaggttag agtcgatggt
     2161 cgccatccct ttgtcgtcac gcgtgctgct ggcattggtg acccctgcgg caacactatc
     2221 aacagtccct ttcagcactt tgttactgcc aagcggcgtg atctccgcac gatacccagg
     2281 gcgcacccct tccagcttag tttcttccat ataggccagt acatagaagg agttctgttt
     2341 caccagcgca accgccgttg atccacgggt aataaactca ccggtataga cgttgaggtt
     2401 ggtcacccag ccatctgctg gcgcgcggat caccgtgcgt tcaagatcca gttttgccag
     2461 atcgcgggtc gcctgcgctt tcgctaactg atgcagaacc gtttgtagta cgttgttggc
     2521 ctggtcgatc tcttcgcggg acatcgcctg cacaccgaga cggttacgac gtccggcctc
     2581 ctggcgtttc tcctgtgcca gtacctgata ataagcaaca tcggcttgcg cttcctcaag
     2641 cgccttttga tagcgcggct ggtcgatggt gaacagtacc tgtccttttt tcaccagctg
     2701 gttatcatga acattcacct gggtaatgag tccagaaacg tccggcgcga tcgcaacgac
     2761 gtcagcgcta aagcgcgcgt cacgcgtcca cggggattcg gtgtaataga cccaggcatt
     2821 aaaaattgcg atgaaggcca gaatgactaa tacgaccgtg atggccgtac gggagaattt
     2881 tcttattagt gttttcactt caacctcaaa cgaacagtcg cgatatcaaa taaaacaagc
     2941 agcaatagag cgcggtgttg aacaacgccg gatgccagac aaagtcgtag atacctgttg
     3001 gcacaagtac ccggcgcacc agccagaaaa tcgccagtga taaaagcaat tcgaaaaata
     3061 tcggtgggaa ggacagccca aacaccacga taacgggaaa cagactcatg ttgaccttgg
     3121 ttgtatagag agagcaggcg ttattatttt cagcatctgt cgccgcagag aagggcatag
     3181 aaagccgggc gagagcaaca ttgctgtaga ttgatattta atatattaac gtaactgtta
     3241 tgctgttatc tatattatgt gatctaaatc acttttaagt cagagtgaat aatggaacga
     3301 ctaaaacgca tgtcggtgtt tgccaaagta gttgaatttg gctcttttac cgccgccgcc
     3361 agacagctac agatgagcgt ttcgtccatc agtcagacgg tgtcaaaact ggaagatgag
     3421 ttgcaggtaa agctgttaaa ccgtagcaca cgcagcattg gcctgaccga agccggtaga
     3481 atttactacc agggctgccg tcggatgctc catgaagtgc aggatgttca tgagcaactg
     3541 tatgccttca ataacacccc catcgggacg ctgcgcattg gctgttcttc aactatggca
     3601 caaaatgttc tcgccgggct gacagcaaaa atgctgaaag aatacccagg tttgagcgtc
     3661 aatctggtta ccggaattcc tgcccccgac ctgattgccg acggtctgga tgtggtgatc
     3721 cgcgtcggcg cgttgcaaga ttccagcctg ttttcccgcc gtctgggcgc gatgccaatg
     3781 gtggtgtgcg ccgcaaaaag ctatctcaca caatacggca taccggaaaa acccgccgat
     3841 ttaagtagtc actcatggct tgaatacagc gtgcggcccg acaatgaatt tgaactgatc
     3901 gcaccggaag ggatctcgac tcgcctgatc ccacaaggaa gatttgtgac taatgatccg
     3961 atgacgctgg tgcgctggct gacggcgggt gccgggatcg cctacgtgcc gctgatgtgg
     4021 gtgatcaacg agatcaatcg tggggagctg gagatcctgc tgccacgtta ccagtcagat
     4081 ccacgcccgg tttatgcgtt atataccgaa aaagataagc tgccgctgaa ggtacaggtc
     4141 gtgatcaact cgctgacgga ttattttgtt gaggtcggta aattgtttca ggagatgcac
     4201 gggcgcggga aagagaagta attaatattg tactgttacg ttgtacaaac ctgtgccgac
     4261 gggttcccct aaccccagag gggcgagggg accgttcgtg cacggagaag gattaattat
     4321 ccttctgaaa atactgaaat tattacgcag taccgccaac agtcaggtta ttaactttca
     4381 gcgttggctg gcccacgcca accggcaaac tttgcccttc tttaccgcag acacccacgc
     4441 cgttatccag tttcaggtcg ttgccaacca tcgaaatctg ctgcatggtt tcgataccgg
     4501 aaccaatcaa cgttgcgcct ttcaccggct tagttacttt accgttttca atcagatacg
     4561 cttctgaggt ggagaaaacg aatttgccgg aggtgatatc cacctgaccg ccaccaaagt
     4621 tcggtgcata gataccgtac tcaacggatt caataatttc ctgcggggtc gatttacccg
     4681 gcagcatata ggtgttggtc atacgcggca tgggcagatg ggcgtaggat tcacggcgac
     4741 cgttgccagt cggcgtcatc cccatcaaac gcgcgttgag tttatcctgc atgtagcctt
     4801 tcagaatgcc gttctcaatc agcacgttgt actggcctgg cgtaccttcg tcatcaatcg
     4861 ccaccgaacc acggcgatcg accatcgtgc catcatcaac cacggtacac agttctgaag
     4921 ccaccaactc cccgacctgt ccactaaata ctgaagtgcc acggcggttg aagtcgcctt
     4981 ccagaccgtg acccaccgct tcatgcaaca gcacgcccgg ccaacctgcg ccaagtacta
     5041 ccggcatggt gcccgctggt gcagcaacgg cagaaagatt gaccagcgcc atacgcactg
     5101 cttcttttgc ccacgcatcc gcacggactt cgccgtcgag atcggcaagg aagaactcat
     5161 aaccaaaacg accgccgcca ccactggcac cgcgttcgcg tttgccatct tcttcgacca
     5221 aaacgctcac ggaaagacgc accagcggac ggacatccgc cgctagtgtg ccgtcggtgg
     5281 ccgcaaccag aattaattca tagacaccac tgaggctggc agtcacttcc tgtacgcgct
     5341 tgtccgcttc gcgggcaacc ttatcgacgc gacgcaggat atccaacttc tcttcacggc
     5401 tcatgctttg cagcggatct accgaggtat ataacgggct atgctctacc gcgcccagcg
     5461 tctgtacttt gccatcacca ctatcacgga cgatggtgcg cgccgcttgt gcactctgtt
     5521 ccagcgccag caggctgatt tggtcagcat aagcaaatcc ggttttttca ccactgattg
     5581 cacgcacacc aacgccctga tcgatgttgt aagaaccatc tttaataata cggtcttcta
     5641 aaacccagga ttcgtgatag ctcgactgaa aatagagatc gccataatca aggcgacgtt
     5701 cggccagttg accgaggatc gcgaacaagt cctgatgttt caggccgttc gccgctagca
     5761 attgttcact taccaggttc agactcatcg tttttgctac tcgttagtta ctgcagtaga
     5821 ggatttttta cggctgccgg atgcggcgtg aacgccctgt ccggcctacg gttatgttct
     5881 ggctcgttgg cctttggcaa cgattatcat atgagattgg ggcaattacg cgccctcgtc
     5941 aaatcattgc gctttttctt tacgcggttg tcgcaacact tcgttgattt gcggatcgtc
     6001 cagcggaccc gaaatgtgat agcgcaaaat ggagactttg ctccacagcg gtcccagcac
     6061 tttactggcg gcaaacactg ccgcgccaac aatggggtta accgcaaagg ccgcagccac
     6121 acccaccgtc gcagaaatct ctggtgcgac aaccgcttcc atattcaggt cgcgacgtac
     6181 cagatttacc gaccctttca tggcgatgtc cgcttccagg ccatccacca gcgtgtcgtc
     6241 ggtgtgcata acgccgtctt taatccacgc ggtgctgcga atggagtcaa aatagaaccc
     6301 ttcgccaaaa gtgtctctga aatcaaaacg cagcttacgc atcagggcat ctacgctcaa
     6361 taagcgcagc aattgcccgg catgtccggt attgatttcg gtaatttcgc ctttacccag
     6421 ttgagtatga atgatgccat tcaacgtcgc ctcatctggc tgccagggtg ctttgcgcca
     6481 gtgtaaatcg taatccacat taaatgacga ctggcgtatg ggcgtcgtga caccaaaaaa
     6541 ttctgcggcg gcatcaattt tctgcccgcg cagttttcct ttcagcgagg ttcgttcatt
     6601 ccccggattg ttaacccatt caccatcggc agtgagccgc gcaaaaccag tatcaatcag
     6661 tccattagtc agcgttaacg tatcgccaga aatggtgata tcactgtcaa tgcgaccgaa
     6721 tttttgcccc cagaaccagc actctgcgca tcgtatttgt gcgtctggcc agccacggaa
     6781 gctaatgcgt tccgttgtcg ggaaaggtga tgactgtgtt gaatcaccac gagttttcgc
     6841 cacgcttggg ttgtaataaa gatatttgat attcgccagc cacggcgcgt tattacgcat
     6901 cgccagcgtg gcgttgattt cacgcccttg cgcctcaacc agggtgccat ttgccgtcgg
     6961 ttgcgaaaca atactcaggt tattccattg ctgatttccc agcgacaaca taggcgtacg
     7021 taacgttatg tgttgtggga aactcgctgc accaccgaca ctctccgccg cgcctttctg
     7081 gaacagggcc agccactcgg caccattcat cggcggcata ttgagttcaa cgccactttg
     7141 ttccggcaac ggcgggagcg ttttactgtc tgccgcccaa atagcacgat cgagcgtcag
     7201 cttttgaccg agcaaccagc ggctattgaa atagttatcc gcaccagcct gtccggttaa
     7261 ttcaaagctg ttgagattgc catcaacctt aacgtttacc gatagtggtt cacccgcagg
     7321 tttggctaac ggtgaaggta agtgactgct cacattcttc aaatcgccgt tcagctctac
     7381 gttatacgtc gcgccagcat gataaggcag ctcaatgccc actttaccat cccacgccac
     7441 gctgccactt aatgcttcgt tcaccgctgc aggcagaacg ccggttttcg ctggttgcca
     7501 gttaccgttg agattcaccg cgacctggta ggcttttgcc ccttctttgg tggaaaaatc
     7561 cacgttcaac ggctgattaa accagcttgc tgtcagtggt tcactttgca gatcgccatt
     7621 gataaagctg aatttaccgc tcaaattttt cagggtgctg tcgagtggtt tgataaacag
     7681 actgttatta cgcagcgtca cttcaccttt cgcggttacc aactcgccgt tcagcgggat
     7741 atcaagatgt aagcgagcat tcacatcgcc gtcgagctgg agttcttgca gggtcgcacc
     7801 cagggaatct ttcagcggtg tctcatcaaa gtaagggcca acggctttac ccggaccttt
     7861 aatgtcagcg tcaatcagca gtttttcttt tgagtagtca gggatcactg cggtgagatt
     7921 actcgcgcgc acgccgccca gattaacgcc atcggttttc atccataaac cgtcgttaat
     7981 aaagtccagt tcaatatcaa ggttagttaa tgcaggccag tccggctgga aggcaaactt
     8041 cgcgttgcgc agcggcacca gcacttcaaa ctgaccttcg ttgtgtttat aggggaagag
     8101 ttgcggattg ccaccataaa ccagcgtcgc gttatccgct tcaccgccct gaatcgcgcc
     8161 acttaagtaa tcaaccaggt ctttacccat caagttttcc gggaaatagc gccaggcttg
     8221 tgaaccatca tcggtactga tgccagccag aatacccagc cagggttcat cgttagcagg
     8281 ttgcaggtaa cgaaaaccgc cgcgcgcatg gacggctttg gctttaacgt caatattacg
     8341 cccatccagc tggaaacctt tgtcattgtt cagccagctt atcgttgcct ggccgtcggc
     8401 gatttccagt ggcgcacgga ataccgtttc gtaaggcatc tttgcctgct tcatcgacgc
     8461 agtaagcaaa ccattttcta cgctgccgga aagcgtcccg gagaagtgtt ccgcacccgg
     8521 taataatttc cattgcttcc aggccagatc gctccacgat gcctgaaaac gggtcttgtc
     8581 tgccgcctga agcgggatat ccagcgccag agtgttaatc ttgccgctcg gttgtgtgga
     8641 gcgccaaaca tcacccagtg caggtgaaag tttcgcgacc agcgggcgta cgccctccag
     8701 ccctgccagc tccagattac tggcacgaat ccggagttcg tcactgcgtt tattgtcttt
     8761 gccgccaacg tcctgttccg gtatccaggc cagcgtcaat gctccgctcg gccagggttt
     8821 gccgtccatc gtgatccgtg tatcgggaat agagaactgc catcccggat tttcacgcgt
     8881 aatatgcgca gtcagattat ccaccgacag cgtatgcgtt tccttctcgc ccaaccagct
     8941 ggcaccgccc tgtttcagcc agacgtcacc gccggttaca tcgcctttgt cgatcgtcat
     9001 ccagccttca agggagaact gtgccgtttc cagcgcaata ttgtcctgca tccatttacc
     9061 gagccacggc ttcaggtcga tgtcatccgc ctggagccag acgcgaccat tgcttaacaa
     9121 cccctcatca tcgcgcaaat ccatacgcac ctgcatcacg ccgtgctgtc cggtaaggct
     9181 ggagaggctt accaggcctt ccgcacggtg tcgacgtgga tcgttcagcc aggtgagctg
     9241 tgggatcgcc agctcggcgc gctgaccgga tggcgtcagg aaactgactt cactgtcgcg
     9301 gagatcgaaa tggtcaaatt gacgaagaaa cagatcgctg atgtgactgg cttccaggct
     9361 atcattacca ccgccgctgg tgatagaagt gttggtgcga aagcgcagct gccagaaagt
     9421 gaggtcgcga aactgccagc gcatatgtaa caggctctgc cagacatcca gcgccagggt
     9481 aacgcgttta accgaaaatt cgccgccatc ttttagttct gcacggatgt cgtgcgcttc
     9541 aagcgtcgga ccaaaattct gccagctggc tgagagctga ctggcttcta ccggcatgcc
     9601 agtcgcggat tctattttgt tgaggatttc cggacgccag gcgtcaagat gcggtaaagc
     9661 aatacgcagg ccgctaacca gcagggcagc gatcacaacg agcgcggctc cagtaagcag
     9721 taaaatcccc ggcaatcgcc tcacccgtca ctccttgtct gctaaaaatg tgactcaaaa
     9781 accctttgcc ggatggcggc ccagcatctg tttacatcat tacgacgtca aactgctcct
     9841 ggttatagag cggttcaatt tgtactttaa cctgtttgcc aacgaaaatt tccacttccg
     9901 ccagcgagtg tgactcttcg cctttcaagg cttcggctac tgccggagaa gcatagacca
     9961 ggaaacggtc ggagtcgtaa gcatggtgga cacgaacaat ctcgcgcatg atttcatagc
    10021 ataccgtttc cacggttttc accgttccgc gaccgtggca ggttgggcat tcgttacaca
    10081 gtacgtgctc aatgctttcg cgggtgcgtt tacgcgtcat ctccaccagc cccagcgccg
    10141 aaaaaccatt aacgctggtt ttcacccgat ctttgctcaa cgcctgctcc agcgagtgca
    10201 gcacgcggcg acggtgatct tcattattca tatcgatgaa atcaataatg ataatcccgc
    10261 ccagattgcg caaccgtaac tggcgagcga tagcctgcgt cgcttcaata ttggtattga
    10321 aaatggtgtc gtccagattg cgatgaccga caaacgcccc ggtattgatg tccacggtgg
    10381 tcatcgcttc ggtctggtcg ataatcagat aaccaccgga tttcagttct actttgcgtt
    10441 ccagcgctcg ctggatttcg ttttcgacat caaagagatc gaaaatcggc tggcgtccgg
    10501 tgtaatgctc cagcttgctg gtcatctcgg gaatgtactc tgaggtaaac tccagcaatg
    10561 cttcgtaagt caggcgtgag tcaacgcgaa tgcggtcgag ttcggcgtcg gcgaaatcac
    10621 gcagaacacg ctgcgccagc gccagttcgc cgtacagctg ataacgggtc tgcgggcgtt
    10681 ttttacgctc cataactttg gtccagacgc gtttcagata agcagcatcg gaggccagtt
    10741 cggcctcgcc aaccccttcc gctgcagtac ggatgataaa cccgccctgc tcgtcgcaat
    10801 actctgcgac cacttttttc aggcgttcac gttctgattc gctttcaatt cgttgggata
    10861 ccccaacgtg agaagcccct ggcataaaca ccagatagcg agaaggcagc gtgatatcgg
    10921 tggtcaggcg cgcaccctta gtgccaagcg gatctttcac cacctgcacc atcagatctt
    10981 gcccctgacg gaccagttcc gagatgtcgc gcaccgtgaa ttgcttttgt tcttcacccg
    11041 ccacacattc ggtgtgcggc atgatgtcag atgcatgaag aaacgcggct ttatccagcc
    11101 caatatctac aaaagccgcc tgcatacccg gaagtacacg acttacacga cccttgtaga
    11161 tattgcctac tattccgcgt cgcgcctcac gttcaatatg aatttcctgc agaataccgc
    11221 catcaatata cgccactcga gtttccgaag gcgttacgtt taccaacaat tcagccgtca
    11281 tgtttatccc ttttctcacg cagtgcgtta aaattactta ataattcata cgtttcaacc
    11341 agcggtaagc cgactacggc gtgaaagctg ccatttatct tcctgacaaa acagccaccc
    11401 agcccctgaa taccgtatgc acctgcttta tctaacggtt cgccgctggc gacatagccc
    11461 gcgatgtctt cgtctgttaa cgttctgaaa gtcacatcgg tgaccaccag gcaatcgaga
    11521 atgtgctggc tgtcggccaa cgccactgcc gtcatcacct gatgggtctg acccgataat
    11581 ttgcgcaaca tctgcgccgc atgctctgcg tcgcgcggtt tctcgagcac ttctccattc
    11641 aggataacga tagtatccgc acccagcacc gggagatcct gcgccgtttg cgcgacaccc
    11701 gcctgtgctt tctcgcgcgc cagacgcaca acatactgct gcgcgctctc ctgcggctga
    11761 cgctgctcct caatgcccgt aacaatacgt tcaaaggtca cgccaagttg cgcaagtaac
    11821 tcctgacgac gcggagaacc ggaagctaaa tacagagaag tcatagaaac ctttattgca
    11881 ctgcaaactg ctgacggact ttacgcatca gcaagaaaat ccacggccag agaacgccat
    11941 tgactacact actccagaac acttccggtc tgaaagagac gttaatcact aaaaactctg
    12001 cccagaaaac aataatatcc accaccagcg aaagcaacat gacgaccagc gcctgctgcc
    12061 ataatgcgag gttgcggaaa agctggtatt tcagcgccac caggtaagca atgatgctca
    12121 tcgccaatac gcgtacgcca agcgtcgagc cgctgatcag atccagtatg gcacccatca
    12181 caaaacctgt gcccacattt acgcgatggg gcaaggccag gatccaatac aacaagatga
    12241 gtaacaccca gtttggccgg aaaacaatca ggttgtccgg ccatggcatg atttgcagca
    12301 acagcgcaat gaggaaagag agccagatta cccagcgtcc ctggctacga tagctcgcca
    12361 ctattgtcct cccggcgcac gcgcaggcgg ttgagcacca ctttgcggcg gcgtagccct
    12421 ttgtggagag cgattagcag cgggctgtgt gggcgcagca ggcgcagtaa ctgcatttcc
    12481 tgtcgccggt tgctgcggag tcggctgagt gatccccgtt gccggttcag gtaactttgg
    12541 ccccatcgcg tctggcgaag gcaatacctg cggcatcatc tgcatcagac gttcattagc
    12601 aacacgatgc acctcttccg gcgtcatcgg gttagcgccg ttacgatccg cgccccacag
    12661 cagcagcaga taacgcaaac gttgcagtcc tgcagtcgga cgcgcctgaa tcacagtata
    12721 agcgcgctgg gtatcgagtt ttacggaaga gacaaccgcg accggatagc cttccgggaa
    12781 acgaccgccc agaccggaag tcaccagcac atcaccaaca cgaatatccg tattcgctgg
    12841 cagatgctca agctgcaaat catccgtaca accgttaccg gctgcaatta cgcggatatc
    12901 gttgcgcagc acctggattg gcagcgcgtg ggtcgcatca caaatcagca gcacgcggct
    12961 ggtcagttta gcgacggcca ccacctgacc aacaacacct ttgtcgctga taaccggctg
    13021 gccttcataa acgccattaa cgctaccttt atcgataaca acttgatcgc tataaggatc
    13081 gttaaccgtg gagataacct gagtcaccat tttctgctca tcctgacgca gcggggaacc
    13141 cagcagctcg cgcagacgcg cgttctcctg tttgtattgt ccaagcatca gcagttcact
    13201 gtttttcagc aacagttcct gacgtaacgc ccggttttca agttctagtt ggtcacgcga
    13261 ggccagcgtc tgcgatacgc catccagcaa ttcacgagga gcattggaaa caaagtagaa
    13321 aggactgacg gcggtatcca tataagtacg gatttgactg aacgtcccca ggcggctgtc
    13381 ggcaataata atgccgagcg ccaccagcac cgccagaata aggcgaatct gtagcgacgg
    13441 gccacggcta aaaattggct tcataagtta tgcgtattct cgtatcagac caggcagggt
    13501 aaacagacac ttcccctgcc tgcatccgat tactcttcgc tgaacaggtc gccgccgtgc
    13561 atgtcgatca tttccagcgc tttgccgcca ccgcgcgcca cacaggtcag cgggtcttca
    13621 gcaacaacga ctggaatgcc ggtttcttcc attaacaaac ggtcaaggtt acgcagcagt
    13681 gcgccaccac cggtgagcac catgccgcgc tcggagatgt cggaagccag ttccggcggg
    13741 cactgttcca gtgcaaccat taccgcgctc acaataccgg tcagcggttc ctgcagtgct
    13801 tcgaggattt cgttggagtt cagggtaaaa ccgcgtggaa caccttctgc aaggttacgg
    13861 ccacgaactt cgatttcacg gacttcatcg cccgggtaag ccgaaccgat ttcgtgcttg
    13921 atacgttctg cggtggcttc accgatcaga gaaccgtaat tacgacgtac atagttgatg
    13981 atagcttcgt cgaaacggtc accaccaatg cgcacggaag aggagtaaac cacgccgttc
    14041 aaggagataa cagcaacttc agtagtacca ccgccgatat caaccaccat agaaccggtc
    14101 gcttcagaaa ccggcaggcc agcaccaatt gcggcagcca tcggttcttc aatcaggaag
    14161 acttcacggg caccagcgcc ctgcgcggat tcacgaattg cgcggcgttc aacctgggtc
    14221 gcgccaaccg gcacacaaac cagaacgcgc gggcttggac gcataaagct gttgctgtgc
    14281 acttgtttga tgaagtgctg gagcattttt tcagtcacga agaagtcggc gataacgccg
    14341 tctttcattg ggcgaatggc agcaatattg cccggcgtac ggcccagcat ctgcttcgcg
    14401 tcatgaccta ctgcagctac gcttttcggt gaaccggcac gatcctgacg aatggccacc
    14461 acggaaggct cattcaatac gatgccttgt ccttttacat aaatgagggt attcgcagta
    14521 cccaggtcaa tggacaagtc attggaaaac atgccacgaa attttttcaa catactaagg
    14581 gataatcctg aaagctgggg cggaaaagaa aatccgctta ctttaccaac cacacgcagc
    14641 agcgacaagg cgcaaaaatc atctgctacg gtgaaaatta gtgcagttcg tttcctttgt
    14701 tacaaatctc tgcctgagtc cagaaaggct taatgcatca gcagcattcc tcgcctgttt
    14761 gcaaccgcgt aaggtcattc atctgcatat gtgctgcaac aatctggcga gcagacaagc
    14821 acactcccat gagacgcagc gcgcgttatt ctacgtgaaa acggattaaa cggcaggtta
    14881 aaccgagtat ctttgtgaat attttttcac gttagtatca agtggctgtg aggacgcgaa
    14941 aaaatcccct tgcccacctg taacaccgcg ctgaatcagg gtctgccact cgcttcgcga
    15001 acgcacgccg gtggcgtaaa cctgggtgct ggtcccggag caggcttcca ccaggctttg
    15061 aaccagcagc tggttctccg ttcgcttctc aatgtttctg accagccccg gatggagctt
    15121 gagtaactca acattaagtt ctttgatcca actggtgctt accagcgtca aaccagcctg
    15181 gttgacggct acccgtaccc ctaaagcatt cactaaacga ataacaggtt gtaagcgact
    15241 gatatgttga ccaacatcgg cctctgcaag ttcaataatt atgcgtcttc gttgcgattt
    15301 ttcacattgc attaacgtat cgcgcagcca acgctgaaaa cgcgggcgaa tcagcgactc
    15361 aacggtaacc tgaatcgcca gattttcctc tggccagtaa cgcaatagtg gaataagacg
    15421 gctgattagc aagcggtcat actcttccga taaaccaaac tgcaagacca tcggcatata
    15481 ctctgccgag ctaacctctt cattaccatc gaagatgcgg cacatgagtt cacgatgatg
    15541 aacccggcct tcgcgagtaa ccgccggttt ttgataaagg cgcgggccgc cgcgactgag
    15601 catttgctcg ataagcgtac gccagcgaac attaccgcgt cctttttcag gcaacgagtc
    15661 atcgtaaata gcccagctat tgccgccctg caatcccgca ttacgcgtgg cagactctgc
    15721 atgttccatt acctgctcgg tatcctgacc actacgccag gcacagatac caatgtggat
    15781 catatcgtcg cgatcgagca ttttattgtt cggcaaggta tcgacggctt tgattaactg
    15841 actggcgatg ctctctgcct cttttagcgt ccggtgcggt aacagcgcag caaaatcact
    15901 gcgatggtaa cgcgccagca gtgcgccagg gtagcgcatc ataaatgtcg atagcagatt
    15961 cgtcagagag aagaattgtt cttcaaccag gctgtgcccc caggtatcac tcaacatatt
    16021 gaaatccggc agacgaatca tcatcacgat cccgtgggta cctactttct cttgatcttc
    16081 cagtaacgtt gctaactgat tatcgaaaaa gagtcggtta ttgaggccgg ttttcatgtc
    16141 ctgggcggca taagagcgga tcagcgtatc aagacggctg tgttgttcgc gtgcgttctg
    16201 aatttcacaa agcagcgtat ccagcgcact gctggttctg ggcggccatt catagatggt
    16261 tcccaacaca ttagagccac gctcaccgtt taagatacga gtagcccggg tttccagcaa
    16321 ttcttgcccg gcaagttgcc gttgtaacca gcgtaccgcc aggaagagca taagaatgat
    16381 aaagccaatc gcccccgtga gcggcgcggt ggtcattaaa gaatggaaat agttgcccat
    16441 cggatcctga taaaccagac gcagcgacat ccccggatgc tttatcaacg gaacgctcag
    16501 ttcacgaaac agatcgttgg tgccaaccgg acgataacta ccatttctgg ccagggtata
    16561 aacctgctta tcaccatgga gcaggtctac acgaacgata tctgccgaca tcattaattc
    16621 ggtaatttgt ggccttaatg tgctgaagtc attcgacagt aggtgggtat caatcgccgt
    16681 cgccaccgcc tgaacacgat gactaaactt atactgaatg gcgttgtaga aacttagcga
    16741 acagcccagc aaagtcacaa aaattgttaa cccggtgagc agcgtgacaa aggccgaaaa
    16801 tttcgtcgtt aatctcatcc ttgtgttaac tccgatagtg aggaagcggg catactagca
    16861 aatcagattt atctcgcaat ttattgcgct tcatcggctt tgcttttcca ttagcgagta
    16921 tagtcttcag aaattatttt ccaatccatc atgcacatga ggaccactta tgcaggcgtt
    16981 acttttagaa cagcaagacg gcaaaactct cgcatcagta cagactctgg acgaaagtcg
    17041 cctgccggaa ggcgatgtca cggttgatgt tcactggtcg agcctgaact acaaagatgc
    17101 gctggcgatt accggtaagg gaaaaatcat ccgtaatttt ccgatgattc ctgggatcga
    17161 ttttgccgga actgtacgca ccagcgaaga tccgcgtttt catgccggtc aggaggtgtt
    17221 actcactggc tggggcgttg gtgaaaacca ctggggtggg ctggcggaac aggcgcgagt
    17281 gaaaggtgac tggctggttg ccatgccgca agggctggac gcgcgtaaag caatgattat
    17341 cggtactgcc ggttttaccg ccatgctgtg tgtgatggcg ctggaagatg ccggtgttcg
    17401 cccgcaggat ggggagattg tcgtgacggg tgccagtggt ggcgtcggca gtaccgccgt
    17461 ggcgctgctg cataagttgg gttatcaggt cgttgctgtt tccggtcgcg aaagtaccca
    17521 tgaatatctg aaaagtttag gtgccagccg tattcttcct cgcgatgagt ttgccgaatc
    17581 ccgtcctctg gaaaaacaag tctgggctgg ggcaattgac accgttggcg acaaagtgct
    17641 ggcaaaagtg ctggcgcaaa tgaattacgg cggctgcgtg gcggcctgtg gtctggcggg
    17701 tggttttact ctgccaacca cggtcatgcc atttattctg cgtaatgtcc gtttgcaagg
    17761 ggtggattca gtaatgacgc caccagcacg ccgtgcacaa gcctggcagc gactggtcac
    17821 cgatttaccg gaatcattct atacccaggc ggcaaaagag atatctctgg cagaggcacc
    17881 gaagtttgcc gaggccatca ttaataacca gatccagggt cgcacgctgg tgaaggttaa
    17941 ctaaccattt agcagggaat aataagagag ggaactcatt ttgaaatcat tattcaatca
    18001 gttaacggga aaagcggtta gccggacagc tttcgtcgaa catcttggtt atgaagttgt
    18061 acaacatcat ccaaactgga aagtcatgat ttcgactgac cataaattga tgcgcattga
    18121 tgctccacca aacagccatt attgatacgc ctccgtcgcc tgttaggttt atggtgcttt
    18181 gccttggcga cgctacgctt aatctgttac tgatttctgg taccatgggg ggaataatct
    18241 gattttattt gattacaaat taaccactta aacctattga atgcggagca ttgtcagtcg
    18301 gttaattttg cgtgctttag cattcacatc tttccggacg atgcagtgaa aattgggtaa
    18361 tccccagcaa ccgctgcgta atgtcgtcta tcttgtcgcg atcctggcat ccctacatta
    18421 tttgtggtct gtgaagatta tctcattgca gcccccatct tcgcagggct ggctttacag
    18481 cgtttcacct tacgttataa gaagttccgt cgatgatggc gctaatttcg tgaattgtgc
    18541 ggcttgttgc aaattacacg gtgttgaagg ttatttacat gttagctgtt gattatcttc
    18601 cctgataaga ccagtattta gctgccaatt gctacgaaat cgttataatg tgcgacctcg
    18661 tcctccctga cgcagttttt gcactgcgga aaaggtgaca ttggcgcaac gaaggtatat
    18721 tttgtttttt gccggaggat agcagcagat cgctgcacaa tgtccgtcaa gtctaacatt
    18781 gacactctgg ggcaaaatag accggcgtcc cggcctgctg gaatttatcg ctatgcatac
    18841 agctgtcggg gcatacgctt tacagacggc ggtgaaacgc ctgtcacaat cacactaaac
    18901 aaagagtacg gaacccactc atggatattc gtaagattaa aaaactgatc gagctggttg
    18961 aagaatcagg catctccgaa ctggaaattt ctgaaggtga agagtcagta cgcattagcc
    19021 gtgcagctcc tgccgcaagt ttccctgtga tgcaacaagc ttacgctgca cctatgatgc
    19081 agcagccagc tcaatctaac gcagccgctc cggcgaccgt tccttccatg gaagcgccag
    19141 cagcagcgga aatcagtggt cacatcgtac gttccccgat ggttggtact ttctaccgca
    19201 ccccaagccc ggacgcaaaa gcgttcatcg aagtgggtca gaaagtaaac gtgggcgata
    19261 ccctgtgcat cgttgaagcc atgaaaatga tgaaccagat cgaagcggac aaatccggta
    19321 ccgtgaaagc aattctggtc gaaagtggac aaccggtaga atttgacgag ccgctggtcg
    19381 tcatcgagta acgaggcgaa catgctggat aaaattgtta ttgccaaccg cggcgagatt
    19441 gcattgcgta ttcttcgtgc ctgtaaagaa ctgggcatca agactgtcgc tgtgcactcc
    19501 agtgcggatc gcgatctaaa acacgtatta ctggcagatg aaacggtctg tatcggccct
    19561 gctccgtcag taaaaagtta tctgaacatc ccggcaatca tcagcgccgc tgaaatcacc
    19621 ggcgcagtag caatccatcc gggttacggc ttcctctccg agaacgccaa ttttgccgag
    19681 caggttgaac gctccggctt tatcttcatc ggcccgaaag cagaaaccat tcgcctgatg
    19741 ggcgacaaag tatccgcaat cgcggcgatg aaaaaagcag gcgttccttg cgtaccgggt
    19801 tctgacggcc cgctgggcga cgatatggat aaaaaccgcg ccattgctaa acgcattggt
    19861 tatccggtga ttatcaaagc ctccggcggc ggcggtggtc gcggtatgcg cgtagtgcgc
    19921 agcgacgctg aactggcaca atccatctcc atgacccgtg cggaagcgaa agcagctttc
    19981 agcaacgata tggtttacat ggagaaatac ctggaaaatc ctcgccacgt cgagattcag
    20041 gtactggctg acggtcaggg caacgctatc tatctggcgg aacgtgactg ctccatgcag
    20101 cgccgccacc agaaagtggt cgaagaagca ccggctccgg gcattacccc ggaactgcgt
    20161 cgctacatcg gcgaacgttg cgctaaagcg tgtgttgata tcggctatcg cggtgcaggt
    20221 actttcgagt tcctgttcga aaacggcgag ttctatttca tcgaaatgaa cacccgtatt
    20281 caggtagaac acccggttac tgaaatgatc accggcgttg acctgatcaa agaacagctg
    20341 cgtatcgctg ccggtcaacc gctgtcgatc aagcaagaag aagttcacgt tcgcggccat
    20401 gcggtggaat gccgtatcaa cgccgaagat ccgaacacct tcctgccaag tccgggcaaa
    20461 atcacccgtt tccacgcacc tggtggtttt ggcgtacgtt gggaatctca tatctacgcg
    20521 ggctacaccg taccgccgta ctatgactca atgatcggta agctgatttg ctacggtgaa
    20581 aaccgtgacg tggcgattgc tcgcatgaag aatgcgctgc aggagctgat catcgacggt
    20641 atcaaaacca acgttgatct gcagatccgc atcatgaatg acgagaactt ccagcatggt
    20701 ggcactaaca tccactatct ggagaaaaaa ctcggtcttc aggaaaaata atttgtgacg
    20761 cgcggtgttt tcatcgcgct tagcaaagaa cgtaatcaac gccgggcctg ttaatgctac
    20821 ccggcgtttt tattgccgtt aactaacggt agcctgcaat ttccgctttc aacgcgctaa
    20881 tttcagcatc gccatcaaca attcccagct caaagcagta tttctttgtt tcccctggct
    20941 caataaaggg cagacgcccc tgcgctcgtt ctttatcacg accgtcagga aaactattac
    21001 tgacctccag tcccataaca tattcacccg cgcctaaatt tttccactgg ataaagttgg
    21061 gtagctgcat ggtgtcgaat ttctcgtaaa ttccccaccc cagttcggcg ttcaccagcg
    21121 caactttact catcccctga ctgtcgctat tcaacgtgag atagtaaacc tgctcgtcaa
    21181 aaccgggctg tggggtacaa atctccagat gactggttaa cccttccgcc gcatgcggag
    21241 ttcgtggcgt tatgctttta gccgacagca agatttctgc ctgtggcgat aataaaggcc
    21301 agccgtaatt aaagtgataa agaatttgca gcggctggcg ggtgaaacca tgattagtca
    21361 ccttgtcttc aatgcgcatt acattttcgc cataacggca gcgaatggtc ctttccagcg
    21421 tcagattttc accgaacaat cgtgtttccc gtactttgcc attgatgaca aattcaatgc
    21481 tgtcgtcggt ccgttctgtg cgatacccca cctcttctgc tggagtcgca gccaaacgcc
    21541 cgtgtaaccc cagcgtttct ccttcatcct cacagggtgt gccaatgtag ctaagtccgc
    21601 aggtagtcag taaccccgcg taaaagctac gtaaaaaccc gttgccctga gactcaaaaa
    21661 aagcgggggc gacaacaccg ttgggggtga taaaagagag cgcagtgtcc ttgtagctgg
    21721 cccatgcaat atccagcgcg cgatctttta ccaccgtaaa tgccagtccc gcaccagtac
    21781 ggacatcaaa cgcttctgta ccctttgctc tgccactacg taactcatag tgtttaattc
    21841 cggcaacctg cgagagatcg ccaatccacg accaaatttc gtttttcatt gtgctgcttc
    21901 ccgaatggac atgtcatggc gctggatgaa cgtttcgatt tccataaggc gagacgttcc
    21961 ctccattggg ccacgtcggg ttaccgccag tgcaccgcag gcgttggcat attgtaatgc
    22021 ccgatgggcg tcaaacccta gctggcggca ggcaatccac gcgccaccaa agcagtctcc
    22081 tgcaccggtc gggtcaactt cttccaccgg atagctctca acatgaaact gctcattcgc
    22141 ggagtagtaa ctggctcctt gattgccgcg cttcacaatc acctctttaa caccttcttc
    22201 aagaaaacca gcgattgcac gctccggcgt cgagtgtggc gacaagagca acacttcccc
    22261 ctcactgggc atatagatat ccgttaactc aagaacaaag tggagggcat cgcgcatttc
    22321 tggaatatca agcatttctt tacgaatatt cgggtcgaaa gaaataacgc caccatttgc
    22381 tttaacgatg gttactgctt tttttactgc gtctaccata tggaatgaaa acagggatga
    22441 acccataata tgaaaatggg tacaatcctt cagaatattt tcatcaacat gctgtgccga
    22501 taatttaccg caagcggcat tcttgatatt aaaaataaaa tcgcgatcgc cagagttatg
    22561 ataagtaaca aaggcactac cggtggcttc cagaggtaag actgaaatcc ccctgatgtc
    22621 cacaccgtca gcagccagac gatgtatatt aatatcacca aagccatcat tccccacgca
    22681 gctaatgatg ccgcacggaa cgccaagacg tgtcacctgg tcgataaaaa tagcgggcgc
    22741 accactggga taagggccgt tccagatacc tggttgatca aatggctggc cgattttact
    22801 cgccataatt tcaaccagaa tttctccaat tgtgaagacc ttactcatat gattaattcc
    22861 ttgaaatgat tagctgcgtt ttcttttatt tcttacgtca aaataaacgg ctacaagaat
    22921 gactaatcct ttaaccacca tctggtaaaa ataaggaaca ttcattaagt tcatgccatt
    22981 attaataacg ccgatggtta atgcgccgat caccgtcccc accacggttc catagccgcc
    23041 gttcaggctg gtgcctccca gtacagctgc ggcaatagca tccagttcgt agctcaacgc
    23101 ggaattcggt tgcccggaat agaggcgtga ggcaaggatc atcccgctga tacctgccag
    23161 cacggctgta atcataaaga ctttaatttt caagcgggtg acatttatcc ctgaataaat
    23221 tgccgcttct ttattaccac cagtgatata aatttttcgt ccaaaggttg ttttagtaag
    23281 aatgaagtgg tttatcagca ataagatgat taatatccat ataggaatag gtatatagag
    23341 aaattctcca ttccccagcg ccagaaaggc atcatcttta atcatgatgg gcataccatc
    23401 ggtcactata taagcgatac cacggaagat ccccatagta gcaacggtga caataaacga
    23461 agggatcccc gctatagccg tcaagccacc atttatcatc cccagagcca gtgctgcaat
    23521 aatggataat gggaccaata aaacaaatgg aattccccat gaagccccaa gcgcagcaaa
    23581 tgtgccaatc aatgcaatgg ttgaaccgac cgataaatca atatcgccaa gcaaaagcac
    23641 ataagtcatt ccataggcgg taattgcaat tatcgaaacc tgaagaataa tattagtaat
    23701 gttattggcg gtaaaaaaac tgtcattaat aaaactgaac agaatgatta acaccaccat
    23761 tccactgagg ataccaccat attgcctgaa gactttttta aaattatttg caatgggaat
    23821 tcccgaaggt ttcggactgg caagagtttt ctctgtcatt taacgtactc ctgttgcagc
    23881 ggtcattacc tgtgtctgcg ttaaattttc acatgaataa atatcggcaa tagttttatt
    23941 acgcattacc accagacgat cgctaagtgc catgacttct ggcagttcag aggacaccag
    24001 aataattgct gtccctgccg cagccagttg acgtatcacg ttatagattt caaattttgc
    24061 gccgacatcc actccgcgcg ttggctcatc gagcaataaa atacgcggag ttttctccag
    24121 ccacttcgat aacacgattt tctgttgatt accgccgctc agtgctccca ctgcctgatg
    24181 cacagaactc actttagtgt tcattctggc aattgattgc agcgtcttcg cctcttcttg
    24241 tcggtgattg atgagtccca tccgcgaagc gttatattga aacgccacat ggtgcgtgtt
    24301 catattgacg tcgtgcatca gcactaaccc ttcttccttg cggttttcgg tcacaaaagc
    24361 aatgccatga tcaatagcct gtttgggatt agcgatcctg acttccttac catcaatcaa
    24421 cacagtcccg tgatacgaaa cgaggccaaa tagcgccttc atcacatcgg aacgcccggc
    24481 ccccaccagg ccaaaaaatc cgaggacttc ccccggacgc acagcgaaac tgacctcttt
    24541 aaaaagcgaa ggatgggaaa gattttttac ttccagtttt gcaggaacat tttcatccgg
    24601 tttttcccca agacgtgctg gccaaatatt tttcatttcc cgaccaacca tttgggcaat
    24661 caagtgctgg atatcactgt ttttcttttc atgcgtagcg atatactcac cgtcgcggat
    24721 caccgaaata tcatctgaaa tccgcataat ttcatccatg cgatgagaga tataaataac
    24781 cgcacagcct cgctgcttta atttttcaat aatgttaaac aggatctccg cttcattatc
    24841 acttagcgaa gatgtcggtt catccataat cacgacatcc gctgcatagc ttaaggcttt
    24901 agcaatttct accagttgcc tatgtgccat cgacatattg ccgaccattt ccagagggtc
    24961 tagtgggatg cccagttccg cgaggaggtt ctggcaatct gcgagcattt ttttatcgtt
    25021 aatgatgcca aaacgtcgcg gttcattatt ggcataaata ttttccgcca cggtcatatt
    25081 attactcagg ctcagttcct gaaaaataat cgcaatccct aactggcgag aatgctgcgg
    25141 attattaatg gtgatggttt caccacgtag tgttatcgtt ccctgggttg gcggataaac
    25201 accggccagt attttcatta atgtcgattt accggcacca ttctcgccta ataaagtatg
    25261 cacccgccct ttacgtaaag agagtgtaat atcttttaat gcctgaacat gaccaaatgt
    25321 cttcgcaata tgcgaaacat caagaataac atccgtcata atgacctcgg tgtcacaaca
    25381 aaacgccaat aacgttttgt tgtgaggtga agatatttac tatttacctg tataaacgcc
    25441 gggttgtacc gggatcattt tttctaccgg tttaccattg aggtgatcaa cagcggcatc
    25501 aatggcttta gcacccattt gatctggata ttgccgaatc acggccgcaa acgttttttc
    25561 actgtcaacg gcatcacgcg cttctttcat gccgtcaaaa ccaatgattt tgatgttgtc
    25621 gttatgcgca gattttgctg cgataaccgc tgccagcgcc gcatcatcac caaagccaaa
    25681 cagaccattc aggttagcat ttgcctgcat aatgttttgc gcagtcgtta acgcttcagc
    25741 tcgcgtaata ccaggctgtt cagcaacaat tttaatatcc ggtgtatcgc ttaacccttt
    25801 cttaaaacca tcaacgcgcg caaccaccga ttgtagtgcc ggataagaaa taatggctac
    25861 agtaccttta ccactcaaaa gtttacccat taacttgcct gcttcaacgc ctcccgcata
    25921 gttatcggtc gcaacatgcg atacgaccgg aacgccttcc gcagcgacgt caacggtgat
    25981 aacgggaata ccggccttat cggctttaat tatcgctgcc tggacgcctt tcgaatcgac
    26041 cggagagata ataatggcat ccactttttt agtaataaaa tcctcaacat cagaaagttg
    26101 tttgtttaaa tcctgattgg caatagagac acttaaattg acattgtctt tttttgcctc
    26161 ttctttcatg gcattcgcaa gatcaatata gaaaggatgt tgttgtgtta ataatgacgc
    26221 accaatttta tactcttttg ccaacgaact ttgcgagaac aatgccaggc tcaccaacat
    26281 agctgaacca agtaatttaa tatttttcat aaacaatcct cattattttt gtagggtaca
    26341 acgtcttaaa tttttccttc actaccacaa acgccaataa tacgagttac gacttctttc
    26401 atggcggcct ttgcgggctg catatagtga cgtggatcat ttgccgcagg attgttgtag
    26461 aaatatcctt ttaacgcatc ggcaaacgca attttcaatt ctgtcgcgac attcacttta
    26521 caaataccgc gacgaataca gtgctgaacg tcctgttccg ataagcccga agcaccatgc
    26581 aacaccagcg gaatctccac tcgctgacga atctgttcca gccgcacaaa atccagtcgt
    26641 ggttcatgcg catataaacc atgtgctgtg ccgattgcta ccgccaggga gtcaatttgg
    26701 gtacgttgaa caaactctgt agctgcggct ggcgaggtat aagcggaatc tttttcatcc
    26761 acccgcagat cgtcctcctg gccgccaaga cgccctagct cggcctcaac gctaacatca
    26821 aagcgatggc aaaaggtggt aacttcttgc actcgcgcaa tgttgtcttc aaatggcaaa
    26881 tgggagccat caatcatgac agaacggatc cctgcgcgaa ccttgttaaa aatgtcatca
    26941 ctctcttcat gatggtcgag atgcaggact aacggcaggt tgtactcgcg agccagcgat
    27001 tgtactaacg aaattaaact tcccacgcca ccataacgat atgtaccggg tgttcccgcc
    27061 agcataacgg gagagcgaag ttgtgcagcg ctctcaacaa ctacctgtaa agtttcaagg
    27121 ttatgaatgt tgaaagcagg gacggcatat ccctctgact gggctttttt cagcatgttt
    27181 ttgcttgaaa taatgtacat cgcagctcct gttatttgtc gttcgcctta caaaatgaag
    27241 aaagagaatc ttcactttca ttttgaaagc aaactagcac agcaaaagca gcaaaaagcg
    27301 tgatgcatgt cgaacatcat caaaataact tacaaaaaat aacttaattt atttttcact
    27361 ttgcaagtga acccaatgaa aaccctgcat aaatatgaat atattcattt atttttcaat
    27421 gtattaagat attttagctg caattttgac ccatgatttt cttttcgaat tgtcgatatg
    27481 attgaaggat aactttcaaa gaaaaataca aggggctata gtgttacgca ttaaaggcca
    27541 atgaagagtg ttaacgtgcc gagaaaatcg tctaacttac tgaaacgtag attaaaaatt
    27601 gcagaaattg ttgccgccca gggtgaaatc aaagttgatg acttatctgc gcaacttggt
    27661 gtctctggcg tgacgatccg cggcgatctc agttatctgg agcaacaggg atatttaaag
    27721 cgatcatttg gcggtgctat tgcgacacca ctctcctctg aaccagaagc gatacaaccg
    27781 atcgcgccat tgtctctggc acaacaaatg gaaatagcac gacattgcgc acggttaatt
    27841 cgcgatcgtg acacgctatt tcttggacat gggacaatct gccgtaagat cattccattg
    27901 ctcagcgaag tgaaaaaact gcgtctgatc accaacgatc ccgaacatgc gctgctggca
    27961 aatcagttta ttgatggcga aataatactg gctggcagtg aaatgctgcg ccccgacacc
    28021 gcactggccg gaaaacagct tgaacaggca cttcagcatt tcaccattac acatagcatt
    28081 ctggaaatca gccatctgga tgctgatggt actttaaata tcaacgttcc cagactcgcc
    28141 gctgcctggc agctctgttt cgatcatgcc cagaataaga cggttatcgt tgccgccgat
    28201 cctgcgccat caacggctgc tatcgggcat ttgcatcagg cagaaaatgt gatcgtcagc
    28261 cgcagcatta acgaacaata tcagcaacaa ttgcagtcgg ctgatttcgt tatcctttat
    28321 accagtaatg agtgctttac ctggtccaat cgcgaaaggc ttcaggaatg agaggggttt
    28381 tgtgacaata actatctgcg ctatgggcga attgctggcc gagtttttgt cccgcaaccc
    28441 acatcaaaaa ttcactcagc ctggggagtt tatcgggcca tttcccagcg gtgcgccagc
    28501 aatttttgct gctcaggtgg caaaactgtc ccatcgggcc atcttctttg gatgtgttgg
    28561 taatgatgat tttgcccgac tcattataga gcgtctccgt catgaaggtg tcattaccga
    28621 tgggatccat gttatgaaca atgccgtcac aggtacggcg ttcgtgagtt atcaaaatcc
    28681 ccagctgcgg gatttcgtct ttaatatccc taacagcgcc tgcggtttgt ttactgccga
    28741 gcacattgat aaggatctgc ttaaacagtg taaccatctg catattgtgg gctcatcgtt
    28801 gttctcattt cgcatgatcg atgtcatgcg taaagcaata acgacgatca aatcggctgg
    28861 cggcaccgtt tctttcgatc ccaatattcg caaagagatg ctgagcattc ctgaaatggc
    28921 gcaggctctc gattatttga ttgaatatac ggatattttt atccccagcg aaagcgaact
    28981 ccctttcttc gcgcgtcaca aaaatctgtc agaggaacag attgttagcg atcttctcca
    29041 cggcggcgta aaacatgtgg cgataaaacg cgcccagcgt ggggctagct attacaagct
    29101 taaaaacggt acattacacg cccagcatgt tgcaggtcac gatatcgaaa ttatcgatcc
    29161 aacgggtgca ggcgactgct ttggcgcaac gtttatcact cttttcttat ccggtttccc
    29221 ggcacacaag gcgctgcaat atgcaaatgc cagcggcgcg ctcgccgtaa tgcggcaagg
    29281 tccgatggaa gggatatcct cactggcaga cattgaagac tttttgcagc agcactgaac
    29341 ggcaattttc tgtcagatta ttttccctgc ctgaagactt gccgtagaat tgcccgtttc
    29401 tcgtatcccc ccatgaggag gaccccttcc gtggacactc gttttgttca ggcccataaa
    29461 gaggcgcgct gggcgctggg gctgaccctt ttgtatctgg cggtatggtt agtagccgct
    29521 tacttacctg gcgttgcccc cggttttacc ggttttccgc gctggtttga gatggcctgc
    29581 atcctgacgc cgctgctgtt tattggactg tgctgggcga tggtgagatt tatctatcgc
    29641 gatatcccac tggaggatga cgatgcagct tgaagtaatt ctaccgctgg tcgcctatct
    29701 ggtggtggtg ttcggtatct ctgtttatgc gatgcgtaaa cggagcactg gcaccttcct
    29761 taatgagtat ttcctcggca gccgctctat gggcggtatt gtgctggcga tgacgctcac
    29821 cgcgacctat atcagtgcca gttcgtttat cggcgggcct ggagctgctt ataaatacgg
    29881 cctgggctgg gtattgctgg cgatgattca gcttcctgca gtctggcttt cgctcggtat
    29941 tctcggcaag aagttcgcaa ttcttgcgcg ccgctacaat gcggtgacgc tgaacgatat
    30001 gctgtttgcc cgctaccaga gtcgtcttct ggtgtggctg gcgagtttga gtttgctggt
    30061 tgcgttcgtt ggtgcgatga ccgtgcagtt tatcggcggt gcgcgcctgc tggaaaccgc
    30121 ggcgggtatt ccttatgaaa ccgggctgct gattttcggt atcagcattg cgttgtatac
    30181 cgcctttggc ggctttcgcg ccagcgtgct gaacgacacc atgcaagggc tggtgatgct
    30241 gattggcacc gttgtgctgc ttattggcgt ggtacatgcc gcaggtggtt taagtaatgc
    30301 ggtagacacg ctgcaaacca tcgatccgca actggttacg ccacaaggcg ctgacgatat
    30361 tctgtcgcct gcctttatga cgtcgttctg ggtactggtg tgctttggcg ttataggcct
    30421 gccgcatact gcggtgcgct gtatctctta taaagacagc aaagcagtac atcgggggat
    30481 catcatcggt acgattgtag tcgcaatcct gatgttcggt atgcatctgg ccggagcgtt
    30541 aggtcgggcg gtgatccccg atctcaccgt accggatctg gtgatcccga cgttaatggt
    30601 aaaagtgctg ccaccgtttg ccgccgggat cttcctggct gcaccgatgg ctgcgatcat
    30661 gtcgacaatt aacgcccaac tgctgcaaag ttccgctacg atcattaaag atctctatct
    30721 gaatatccgt ccggatcaaa tgcaaaacga gacgcgtctg aagcggatgt cggcggtgat
    30781 cacgttggtt ctcggtgcgt tgctgctgct tgccgcctgg aagccgccag aaatgatcat
    30841 ctggctgaat ttgctggcct tcggtgggct ggaagccgtt ttcctgtggc cgctggtgct
    30901 gggtctttac tgggaacgcg ccaacgccaa aggcgcgcta agtgcgatga tcgttggcgg
    30961 cgtgctgtat gccgtactcg cgaagctgaa tattcagtac ctgggcttcc accctatcgt
    31021 gccctcgtta ctactaagtt tgctggcttt cctggtcgga aaccgtttcg gtacatccgt
    31081 cccgcaagct accgttttga ctactgataa ataaagagtt ttgccatgcc ttggatccaa
    31141 ctgaaactga acaccaccgg cgcaaacgcg gaagatctta gcgatgcgct gatggaagcg
    31201 ggtgccgttt ctatcacttt tcaggatacc cacgatacgc cagtatttga gccgctgccg
    31261 ggcgaaacgc gcctgtgggg cgacaccgat gtgattggtc tgttcgacgc tgaaaccgat
    31321 atgaacgacg tggtggcgat tctggaaaac catccgctgc tcggcgcggg cttcgcgcat
    31381 aaaatcgaac aactggaaga taaagactgg gagcgcgaat ggatggataa tttccacccg
    31441 atgcgctttg gtgaacgact gtggatctgc ccaagctggc gtgatgtgcc ggacgaaaac
    31501 gccgtcaacg tgatgttaga cccagggctg gcgtttggta cgggtaccca tccaactacc
    31561 tctctgtgcc tgcaatggct cgacagcctc gatttaaccg gtaaaacagt catcgacttt
    31621 ggctgcggtt ccggcattct ggctatcgcg gcgctgaaac tgggtgcagc aaaagccatt
    31681 ggtattgata tcgatccgca ggcgatccag gccagccgcg ataacgccga acgtaatggc
    31741 gtttctgatc gtctggaact ctacttaccg aaagatcagc cagaagaaat gaaagccgac
    31801 gtggtggtcg ctaacatcct tgcaggccca ttacgtgaac tggcaccgtt aatcagcgtc
    31861 ctgccagttt caggcggttt gctgggcctt tccggtattc tggcaagcca ggcagagagc
    31921 gtttgcgaag cttatgccga cagcttcgct ctggacccgg tcgtggaaaa agaagagtgg
    31981 tgccgcatta ccggtcgtaa gaattaacct tcgcatcgcc gtatggcgac gcagagcgaa
    32041 gtgcgagcga tgtcacaaaa ggcacgttaa tttgccgatt atttacgcaa atttgcgtgc
    32101 caaaattttc attcataaag aaaaattgag aacttactca aatttctttg agtgtaaatt
    32161 ttagtcacta ttttctaata tgatgatttt tatgagtaat tatcgcgcta cgctcatttt
    32221 aaatgcaatt ctttgatcca tctcagagga ttggtcaaag tttggccttt catctcgtgc
    32281 aaaaaatgcg taatatacgc cgccttgcag tcacagtatg gtcatttctt aactcatgcg
    32341 catcggacaa tatcagctca gaaatcgcct gatcgcagcg cccatggctg gcattacaga
    32401 cagacctttt cggacgttgt gctacgagat gggagccgga ttgacagtat ccgagatgat
    32461 gtcttctaac ccacaggttt gggaaagcga caaatctcgt ttacggatgg tgcacattga
    32521 tgaacccggt attcgcaccg tgcaaattgc tggtagcgat ccgaaagaaa tggcagatgc
    32581 agcacgtatt aacgtggaaa gcggtgccca gattattgat atcaatatgg gttgcccggc
    32641 taaaaaagtg aatcgcaagc tcgcaggttc agccctcttg cagtacccgg atgtcgttaa
    32701 atcgatcctt accgaggtcg tcaatgcagt ggacgttcct gttaccctga aaattcgcac
    32761 tggctgggca ccggaacacc gtaactgcga agagattgcc caactggctg aagactgtgg
    32821 cattcaggct ctgaccattc atggccgtac acgcgcctgt ttgttcaatg gagaagctga
    32881 gtacgacagt attcgggcag ttaagcagaa agtttccatt ccggttatcg cgaatggtga
    32941 cattactgac ccgcttaaag ccagagctgt gctcgactat acaggggcgg atgccctgat
    33001 gataggccgc gcagctcagg gaagaccctg gatctttcgg gaaatccagc attatctgga
    33061 cactggggag ttgctgcccc cgctgccttt ggcagaggtt aagcgcttgc tttgcgcgca
    33121 cgttcgggaa ctgcatgact tttatggtcc ggcaaaaggg taccgaattg cacgtaaaca
    33181 cgtttcctgg tatctccagg aacacgctcc aaatgaccag tttcggcgca cattcaacgc
    33241 cattgaggat gccagcgaac agctggaggc gttggaggca tacttcgaaa attttgcgta
    33301 aacagaaata aagagctgac agaactatgt tcgaacaacg cgtaaattct gacgtactga
    33361 ccgtttctac cgttaactct caggatcagg taacccaaaa acccctgcgt gactcggtta
    33421 aacaggcact gaagaactat tttgctcaac tgaatggtca ggatgtgaat gacctctatg
    33481 agctggtact ggctgaagta gaacagcccc tgttggacat ggtgatgcaa tacacccgtg
    33541 gtaaccagac ccgtgctgct ctgatgatgg gcatcaaccg tggtacgctg cgtaaaaaat
    33601 tgaaaaaata cggcatgaac taattcaggt tagctaattg cttgattaaa aggcgctact
    33661 cggcatgggg aagcgccttt tttataggtg tcacaaaggg agtgaccatg agaacaggat
    33721 gtgaaccgac ccgatttggt aatgaagcaa agaccatcat ccagggtgat gcattaaccg
    33781 aacttaaaaa gctccccgcc gaaagtgtcg atctgatctt tgccgaccca ccgtataaca
    33841 tcggtaaaaa ttttgatggt ctgatcgaag cctggaaaga agatctgttt atcgactggc
    33901 tgtttgaagt gattgcagag tgccatcgcg ttctgaaaaa gcagggcagc atgtacatca
    33961 tgaatagcac ggaaaacatg ccctttatcg atctccagtg ccgcaagctt tttaccatca
    34021 aaagtcgcat cgtctggtca tatgacagtt ctggagtgca ggcgaaaaaa cactacggct
    34081 ccatgtacga acccatcctg atgatggtga aagacgcaaa gaactacacg ttcaacggtg
    34141 atgctattct ggtagaagcc aaaaccggat cgcagcgcgc gttgatcaat tatcgcaaaa
    34201 atcctccaca gccttacaat catcaaaaag taccgggtaa cgtctgggat tttccgcgcg
    34261 tgcgttattt gatggatgaa tatgaaaacc acccgacgca aaaaccggaa gccttgctga
    34321 aacgcattat tctcgcctct tccaacccag gcgatatcgt tctcgacccg tttgccggta
    34381 gctttactac cggtgccgta gccgtcgcca gcgggcgaaa attcattggt attgagatca
    34441 acagcgagta catcaaaatg gggcttcgac ggctggatgt cgcgtcgcat tactctgcgg
    34501 aagaactggc gaaagtgaaa aaaagaaaga ccggcaacct gtcaaaacga agccggttaa
    34561 gcgaagttga ccccgatctc attgcaaagt aaaggaagtg taagcctggt tttcagatta
    34621 ttcatttcgt gtatatttcc tgccagactt ggttaaacat gcacaggctc aggtaatgat
    34681 tcgcaagtat tggtggctcg tcgttttcgc tgtcttcgtt tttctgtttg atactttact
    34741 gatgcagtgg attgaactgc tggcaacaga aacagacaaa tgccgcaata tgaactcagt
    34801 taatccacta aaactggtta actgtgacga actgaatttt caggacagaa tgtgaattta
    34861 tatgacactt aattcattcg ttttatgaat taatttcgtt atgttttcat ctggcatgaa
    34921 cattcttaat acgttatcga ccagagcggg ggcttgttta taaagatcat aacccgccat
    34981 attcattaac cagttttgaa caattccgct gaaggcacca tcaataataa tcatcacaac
    35041 atctaaatcg agattatttg ctacacaacc ttgttgctga cacgcctgca atacttcgcg
    35101 gagagtctgc ggattaaagc ccatcttttc gcgtatcact ccctcggcca gcatctcatc
    35161 attaaattca catttgtgat ataagatttt cagcaacgcc tgctggcggg gaattttggc
    35221 aatatattgc aaaccgacaa tcaatttttc acgcaattgc tgaaacgggt catgctctaa
    35281 cccagcggtc aagtgatcct ggattaactc ccgcaatgaa ggctgttgca accacatctc
    35341 attaaacagt tgagtcttgt tttcgaagtg ccagtagata gcgccacgcg taacgttagc
    35401 agcgtcggca atgtcgttga gcgtcgtctt gcttacgcca tgctgcgcaa actgggcgat
    35461 ggcagtttca atcagttctt gccgggtctt cagggcttca gctttggttc tttttgccat
    35521 gattaattat tcaggaaata aatatattcg acacaggatg agaaaatagc gaaggttaat
    35581 ctatcaccta atgtgtattt atacgagagg ctaatattgc gttgctataa atcgtcaaat
    35641 aaatgatatg tattatttac ataaaataca ttcacttgac ctatacatat ttcaatttac
    35701 gagattttaa ttctatttct ttcattccgc gtcaaaacaa aacagtagaa tattaatctt
    35761 tttttgtgtt tatgtgcctt gagttgccag tattcataac tattccttac atcgacgaat
    35821 gataatttgt aggatagcga actgtatttt tctttctgcg agttaacgca ttgccttttt
    35881 gggcaaataa cgcgcttttg gttttttgag gaatagtaat gacgaaacat gccaggtttt
    35941 tcctcctgcc ctcctttatt ctgatctccg cggctttaat cgccggttgt aacgataagg
    36001 gagaagagaa agctcacgtc ggtgaaccgc aggttaccgt tcatattgtg aaaacggccc
    36061 cgttagaagt taagactgaa ttaccaggcc gcaccaatgc ttaccgtata gccgaagttc
    36121 gcccacaggt tagcgggatc gtactgaatc gcaatttcac tgaaggcagc gatgtgcaag
    36181 caggccagtc cctgtaccag atcgatcccg cgacctatca ggcaagttat gacagcgcga
    36241 aaggcgaact ggcgaaaagt gaagccgccg ccgccatcgc gcatttgacg gtaaaacgtt
    36301 acgttccgct cgtgggtacg aaatacatca gtcagcagga gtacgaccag gccattgctg
    36361 atgctcgcca ggccgatgcc gccgtgattg ccgcaaaagc cacagtcgaa agcgctcgca
    36421 tcaatctcgc ttataccaaa gtgactgcgc caattagcgg acgtatcggc aaatcgaccg
    36481 tgaccgaagg cgctcttgtc actaatgggc aaacgactga actggcgact gtccagcagc
    36541 tcgatcctat ctacgttgat gtgacccaat ccagcaacga ttttatgagg ctgaagcaat
    36601 ccgtagagca aggaaatttg cataaggaaa acgccaccag caacgtagag ttggtcatgg
    36661 agaacggtca aacctatccc ctgaaaggta cgctgcaatt ctccgatgtg accgttgatg
    36721 aaagcaccgg ctccataacc ctacgtgctg tcttccctaa cccgcaacat acgcttttgc
    36781 cgggtatgtt tgtgcgcgca cggattgatg aaggcgtcca acctgacgcc attcttatcc
    36841 cgcaacaagg cgttagccgc acaccacgtg gtgatgcaac cgtgctgatt gttaacaata
    36901 aaagtcaggt tgaagcgcgc cctgtcgttg ccagtcaggc gattggcgat aaatggttga
    36961 ttagcgaagg tctgaaatct ggcgatcaag tcattgtcag cggcctgcaa aaagcgcgtc
    37021 cgggagagca ggttaaagcc actaccgata cccccgcaga tactgcatcg aagtaaggca
    37081 atctgacatg gcaaactttt ttattcgacg accgatattt gcatgggtgc tggccattat
    37141 tctgatgatg gcgggcgcac tggcgatcct acaattgccc gtcgcccagt atccaaccat
    37201 tgcgccgcct gcggtttctg tttcggcaaa ctatccgggt gctgatgcgc agaccgtgca
    37261 ggatacagtg acgcaggtta tcgaacagaa tatgaacggt atcgataacc tgatgtatat
    37321 gtcctccacc agcgattccg ccggtagcgt gacaattacc cttaccttcc agtccgggac
    37381 cgatcctgat atcgcgcaag tacaggtgca gaacaaactc cagctcgcca cgccgttgct
    37441 gccgcaggag gttcagcagc aggggatcag tgttgaaaag tccagtagca gctatttgat
    37501 ggtggcgggc tttgtctctg ataaccctgg caccacacag gacgatatct cggactatgt
    37561 ggcctctaac gttaaagata cgcttagccg tctgaatggc gtcggtgacg tacagctttt
    37621 cggcgcacag tatgcgatgc gtatctggct ggacgccgat ctgctaaaca aatataaact
    37681 gacaccggtt gatgtgatta accagttgaa ggtacagaac gatcagatcg ctgccggaca
    37741 gttgggcgga acgccagcgt taccagggca acagttgaac gcctcgatta ttgctcagac
    37801 gcggttaaaa aatccggaag aattcggcaa agtgaccctg cgcgtaaaca gtgacggctc
    37861 ggtggtgcgt ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact ataacgttat
    37921 cgctcgcatc aacggaaaac cggcggcggg cctggggatt aagctggcaa ccggcgcgaa
    37981 tgctctcgat accgcgaaag ccattaaggc aaaactggcg gaattacagc cattcttccc
    38041 gcagggaatg aaggttcttt acccttatga caccacgcct ttcgtccagc tttctatcca
    38101 ggaagtggtg aaaacgctgt tcgaagccat tatgctggtg ttcctggtga tgtatctgtt
    38161 cttgcagaac atgcgggcaa cattgatacc caccattgct gtacctgtcg tgttgttagg
    38221 gacatttgcc atactcgccg cttttggtta ctccatcaat acgctaacga tgtttgggat
    38281 ggtacttgcc atcggacttc ttgttgatga tgcgatagtc gttgtggaga acgtcgagcg
    38341 cgtaatgatg gaggataaac tcccccccag agaagcgacg gaaaaatcga tgtcgcaaat
    38401 ccagggcgcg ctggtaggta tcgcgatggt actgtcggcg gtatttattc cgatggcatt
    38461 cttcggtggc tcaacagggg caatttatcg ccagttctct atcactattg tttcagcaat
    38521 ggcgctttca gtactggttg cgctgattct cacccccgcg ttatgtgcaa cattgctgaa
    38581 accagtctct gccgagcatc acgaaaataa gggaggattc ttcggctggt ttaacaccac
    38641 tttcgatcat agcgttaatc actacaccaa tagcgttggc aaaatcctcg gttccacagg
    38701 gcgatattta ctgatctatg cgctgattgt tgcaggaatg gtggtgttgt ttttacgtct
    38761 tccgtcttcc ttcttacctg aagaggatca gggtgtcttt ctgaccatga ttcagttacc
    38821 cgctggcgcg acgcaagagc ggacgcaaaa agtgttggat caagtgacgg attactatct
    38881 gaagaacgag aaagcgaacg ttgaaagtgt ctttacagtt aacggcttta gcttcagcgg
    38941 ccaggcacaa aacgccggta tggccttcgt cagtctgaaa ccgtgggaag agcgtagtgg
    39001 tgacgaaaac agtgcggaag cagtaatcca tcgtgccaaa atggaattgg gcaagatccg
    39061 cgacggtttt gtcattccat ttaatatgcc agccattgtt gaactaggca cggcaacggg
    39121 tttcgacttt gagttaattg atcaggctgg gctgggtcac gatgccctga cccaggcccg
    39181 taaccagttg cttggcatgg cggcgcaaca tcctgccagc ttagtcagcg tgcgccctaa
    39241 tggcctggaa gacaccgcgc agtttaaact ggaagttgac caggaaaagg cgcaggcatt
    39301 aggtgtttca ctttctgaca tcaatcagac catttcaacg gcgttgggtg ggacttacgt
    39361 taacgacttc atcgaccgtg gtcgcgtgaa aaaggtgtat gttcaggcgg atgccaaatt
    39421 ccgtatgctg ccagaagatg tcgataaact ttatgtccgc agcgccaacg gcgaaatggt
    39481 gccattctct gcctttacca cttcacattg ggtgtatggc tctccgcgac tggaacgcta
    39541 caacggtctg ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca gttccggcga
    39601 tgctatggcg ttgatggaaa accttgcgtc aaaattaccg gcgggcattg gttatgactg
    39661 gacgggtatg tcatatcagg aacgtttatc gggaaaccag gctcccgctc tggtagcaat
    39721 ttcctttgtg gttgttttcc tgtgccttgc tgcactctat gaaagctggt caattcctgt
    39781 ctcggttatg ttggtcgtgc cgttagggat tgtcggcgtg ctactggcgg caacactctt
    39841 taatcaaaaa aatgacgtct actttatggt gggcttgcta acgacaattg gcttgtcggc
    39901 caaaaacgct attttgattg ttgagttcgc taaagatctc atggagaaag agggtaaagg
    39961 tgttgttgaa gcgacactaa tggcagtacg tatgcgcctg cgtcctatcc tgatgacctc
    40021 tctcgccttt attctcggcg tattaccgct agctatcagt aacggtgccg gcagtggcgc
    40081 gcagaacgca gtgggtatcg gggtaatggg cgggatggtt tctgcaacgt tgctggcaat
    40141 cttcttcgtg ccggtgttct ttgtggtgat ccgccgttgc tttaaaggat aaatcagaaa
    40201 cataaaggca ctttcgggtg cctttattat ttccagtgaa acccataaaa attaataaga
    40261 tattcctcta ctcacttttt aaaaactttc tatagttccc gctcccttca ctatttttac
    40321 aattcacata atttgtcatt attcattccg caagatttat aacctccagt gataaaatgg
    40381 cattcagctc gttaataaga gagttaactt attaagcgtt agcgtttatt actgaggtaa
    40441 caccatgaaa agattaattc ctgtggcatt gctcaccgca ttgctggcgg gctgtgctca
    40501 cgattcgccc tgcgttccgg tttatgacga tcagggtcga ctggttcaca ctaatacctg
    40561 tatgaaaggc acgactcagg ataactggga aacggctggg gctattgccg gcggggcagc
    40621 agctgttgct ggcctgacga tggggatcat cgctctgtcg aaataactaa acagggcgcg
    40681 gagctccgcg ctctttttaa cgatacagta tctttccata agataaaata ttgctatatt
    40741 ctgctgggta attccctgct gatttagcat gttatatcac gttatttcgc tgctttcgaa
    40801 ataacgcatc aatttaactt tcacacttcc caccagaaat atactcacgc taacaatcat
    40861 tattctgctt agtaaaaaac gcgtaaatat tttgcgccaa aatgtggcgc atgtttcatt
    40921 ttcgcaccat tacgaggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac
    40981 gcgaaatcgt gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg
    41041 taaaagacgt ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga
    41101 aaaagatgat gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg
    41161 cgcatgctgg cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca
    41221 gtgatggatt acctgggttc tcttatgccg atgctgacgg taagttttca ggtattgatg
    41281 tagatgtttg tcgtggtgtt gccgctgctg tattcggtga cgatacgaaa gtgaaatata
    41341 ccccgctcac tgcaaaagaa cgcttcaccg ctttacagtc aggggaggtg gatttgctct
    41401 cccgtaatac gacctggact tcatctcgcg atgccgggat gggaatggca tttactggcg
    41461 tcacttatta cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga
    41521 aagaactgga tggcgctacc gtctgtattc aggcggggac tgataccgaa ctcaacgtcg
    41581 ccgattactt caaggcaaat aatatgaagt acacgccagt aactcagacg aatcagcgaa
    41641 ggctctggaa tctggccgct gtgatactct ggcctcggat caatcacaac tgtatgccct
    41701 acgcatcaaa ttaagcaacc caactgaatg gattgtctta ccggaagtta tctctaaaga
    41761 accacttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg tacgctggac
    41821 gcttttcgcc atgctgaatg ctgaagagat gggcatcaat tcccagaatg tcgatgaaaa
    41881 agcggctaat ccagcaacgc ctgatatggc acatctgctg ggtaaagaag gcgattacgg
    41941 caaggatctg aagctggata ataaatgggc ctataacatc atcaaacagg tgggtaacta
    42001 ctcggaaatt ttcgagcgta acgtaggttc agaaagcccg ctgaaaatta aacgtgggca
    42061 aaataatctc tggaataacg gcggtattca gtacgcgccg cccgtgcgtt aagtcggtgt
    42121 gaggtaacga gcgttgctac ggcggcgctc ttttcagcgt tatggttttc gaggtttcta
    42181 tgccccatcg ccgctcaacc gttaaaggct cactctcttt tgccaaccct acggttcgcg
    42241 cctggttatt ccagatcctt gccgttgttg ctgctgtcag cattgttggt tggttatttc
    42301 acaacactgt aacgaatctc agtaatcgtg gcataacatc aggatttgct tttcttgacc
    42361 gcggggccgg ttttggcatc gttcagcatt tgattgatta tcagcaaggc gacacttacg
    42421 gacgcatttt tattgtcggc ctactcaata cgctactggt ttctgcattg tgtattgtgt
    42481 tcgcttctgt tttgggcttc tttatcggtc tggcgagact ttcggataac tggctgctac
    42541 gaaagctttc cacaatttat attgagatct tccgtaatat tcccccgctg ctgcaaatct
    42601 tcttctggta ctttgccgtg ttgcgcaatt tgcccggacc acgacaggca gtgagcgcgt
    42661 ttgacctggc ctttttgagc aatcgtgggc tttatattcc gtcgccgcag ctgggagacg
    42721 gatttattgc gtttatcctg gctgttgtta tggctatagt cctttctgtt gggctattcc
    42781 gctttaataa aacataccag ataaagaccg gacaactgcg ccgcacctgg ccattcgccg
    42841 ctgtgttgat cattggtttg cctttactgg cgcaatggct ttttggcgca gcactacact
    42901 gggatgtccc agcccttcga ggctttaatt tccgcggcgg gatggtttta attcctgaac
    42961 tggcagcctt aacgctagca ctttcgattt atacctctgc atttatcgcc gagattatcc
    43021 gcgcagggat ccaggcagtg ccttatggtc aacatgaagc ggctcgatca ctgggattac
    43081 ccaacccggt tacgctacgc caggtcatta ttccccaggc attgcgggtg attattccac
    43141 cgttaaccag ccagtatctc aacatcgtca agaactcctc tcttgccgcc gctattggct
    43201 atcccgatat ggtttcgctg tttgctggca ccgtgctgaa ccaaacgggg caagccatcg
    43261 agacgatagc catgaccatg tcggtctatc tgattatcag cctgactatc tcgctgctga
    43321 tgaatatcta taaccgccgc atcgcgatcg ttgaacgcta aggagtcatg atgacaaaag
    43381 tattgctgtc tcatcccccg cgcccggcga gccataactc aagccgcgcg atggtgtggg
    43441 tgcgaaaaaa tctgttctcc agctggagca atagcctgct gactattggc tgcatatggt
    43501 tgatgtggga actgatccca ccgttgctga actgggcatt tttgcaggct aactgggttg
    43561 gctcaacgcg tgccgactgc acaaaagccg gtgcctgttg ggtcttcatc cacgaacgat
    43621 ttggtcagtt tatgtatgga ctttacccac acgaccaacg ctggcgaatt aacctggcat
    43681 tactgattgg ccttgtgtct atcgcaccaa tgttctggaa aatacttccg catcgcggtc
    43741 gctatattgc ggtctgggcg gtgatttacc cactggttgt ctggtggctg atgtatggtg
    43801 ggtttcttgg tcttgatcgg gttgaaaccc ggcaatgggg cgggttgacg ctaactttaa
    43861 ttattgcatc agttgggatt gcgggggcgc taccgtgggg gatcttactg gcgttaggtc
    43921 gccgctccca tatgccgatt gtgcgtatct tatcggtcat ttttatcgag ttctggcgcg
    43981 gcgtaccgct gattaccgta ttgtttatgt cttcggtcat gctgccgttg tttatggcag
    44041 aaggcaccag tatcgataaa ctgatccgcg cgctggttgg cgtgatcctg tttcagtcag
    44101 catatgttgc ggaagtcgtg cgaggcggat tacaggcgct gcctaaaggg caatatgaag
    44161 cggcagagtc gctggcattg ggttattgga aaactcaggg gcttgttatt ctgccccagg
    44221 cgttgaagct ggtaattcct gggctggtaa ataccatcat cgcactcttc aaagatacca
    44281 gtctggtgat catcatcggg ttgttcgatc ttttcagtag cgttcagcag gcaaccgttg
    44341 atcccgcgtg gttgggtatg tcgacggaag ggtatgtttt cgccgcgctg atctactgga
    44401 tcttctgttt cagcatgtca cgttatagcc aacatctgga aaaacgtttt aacaccgggc
    44461 gtacaccgca ttgaggacac tatgagccaa attttactgc aacctgctaa cgcgatgatt
    44521 acgctggaaa acgtcaataa atggtatgga caattccatg ttttgaaaaa tataaattta
    44581 accgtgcaac cgggcgaacg gatcgttcta tgtggccctt caggttccgg taaatcaaca
    44641 accattcgtt gtattaatca tctggaagaa catcaacagg gacggatcgt ggtagatggc
    44701 atcgaactta atgaagatat ccgcaatatt gagcgcgtca ggcaggaagt gggaatggtc
    44761 tttcagcatt tcaatctctt ccctcatctg accgtgttac agaactgtac cctggcaccg
    44821 atttgggtac gcaagatgcc aaagaaagag gctgaagctc tggcgatgca ttaccttgag
    44881 cgggtgagaa ttgccgaaca tgcccagaag tttcccggac agatttctgg tggtcagcag
    44941 caacgtgttg ccatagcacg ttcgctgtgt atgaagccga aaattatgtt gtttgatgag
    45001 ccaacgtcgg cgctcgatcc tgagatggtg aaagaggtgc tggatacgat gattgggctg
    45061 gcgcagtcgg gtatgacaat gttgtgtgta acacatgaga tggggtttgc acgaaccgtc
    45121 gctgaccggg tgatttttat ggatcgtggg gaaatagtgg agcaagctgc acctgatgaa
    45181 ttttttgcgc atcctaaatc agagcgtacg agggcatttt tatcgcaggt aatccattaa
    45241 ttgaatatta gttcgaaaag caaaaaggcc atcctttcgg atggcctttc gcctgatttg
    45301 atg
//
LOCUS       DAOFSV010000028        45022 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.028, whole genome shotgun sequence.
ACCESSION   DAOFSV010000028 DAOFSV010000000
VERSION     DAOFSV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.028"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..940)
                     /locus_tag="QBH27_001603"
     CDS             complement(<1..940)
                     /locus_tag="QBH27_001603"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDQ7013927.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(1396..3087)
                     /gene="fruA"
                     /locus_tag="QBH27_001604"
     CDS             complement(1396..3087)
                     /gene="fruA"
                     /locus_tag="QBH27_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDQ7013928.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDFIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(3104..4042)
                     /gene="fruK"
                     /locus_tag="QBH27_001605"
     CDS             complement(3104..4042)
                     /gene="fruK"
                     /locus_tag="QBH27_001605"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDQ7013929.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(4042..5172)
                     /gene="fruB"
                     /locus_tag="QBH27_001606"
     CDS             complement(4042..5172)
                     /gene="fruB"
                     /locus_tag="QBH27_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDQ7013930.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            5540..6721
                     /gene="setB"
                     /locus_tag="QBH27_001607"
     CDS             5540..6721
                     /gene="setB"
                     /locus_tag="QBH27_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDQ7013931.1"
                     /translation="MHNSPTVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(6718..6972)
                     /locus_tag="QBH27_001608"
     CDS             complement(6718..6972)
                     /locus_tag="QBH27_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDQ7013932.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMIWLIDLEMLTAP"
     gene            7127..7699
                     /gene="yeiP"
                     /locus_tag="QBH27_001609"
     CDS             7127..7699
                     /gene="yeiP"
                     /locus_tag="QBH27_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDQ7013933.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            7922..9388
                     /locus_tag="QBH27_001610"
     CDS             7922..9388
                     /locus_tag="QBH27_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ7013934.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGHTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQVIEQAWQQIAQFGAHQALLNTLKI"
     gene            9506..10492
                     /gene="yeiR"
                     /locus_tag="QBH27_001611"
     CDS             9506..10492
                     /gene="yeiR"
                     /locus_tag="QBH27_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDQ7013935.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            10531..11244
                     /gene="lpxT"
                     /locus_tag="QBH27_001612"
     CDS             10531..11244
                     /gene="lpxT"
                     /locus_tag="QBH27_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDQ7013936.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            11656..12222
                     /gene="mepS"
                     /locus_tag="QBH27_001613"
     CDS             11656..12222
                     /gene="mepS"
                     /locus_tag="QBH27_001613"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDQ7013937.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            12403..13959
                     /locus_tag="QBH27_001614"
     CDS             12403..13959
                     /locus_tag="QBH27_001614"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDQ7013938.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            14041..15855
                     /locus_tag="QBH27_001615"
     CDS             14041..15855
                     /locus_tag="QBH27_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ7013939.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKISDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNTWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            15856..16950
                     /locus_tag="QBH27_001616"
     CDS             15856..16950
                     /locus_tag="QBH27_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDQ7013940.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16950..17975
                     /locus_tag="QBH27_001617"
     CDS             16950..17975
                     /locus_tag="QBH27_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7013941.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17977..19566
                     /gene="yejF"
                     /locus_tag="QBH27_001618"
     CDS             17977..19566
                     /gene="yejF"
                     /locus_tag="QBH27_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDQ7013942.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLHIEAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(19570..19914)
                     /locus_tag="QBH27_001619"
     CDS             complement(19570..19914)
                     /locus_tag="QBH27_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013943.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(20248..21438)
                     /gene="bcr"
                     /locus_tag="QBH27_001620"
     CDS             complement(20248..21438)
                     /gene="bcr"
                     /locus_tag="QBH27_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDQ7013944.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(21466..22161)
                     /gene="rsuA"
                     /locus_tag="QBH27_001621"
     CDS             complement(21466..22161)
                     /gene="rsuA"
                     /locus_tag="QBH27_001621"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDQ7013945.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22311..24071
                     /locus_tag="QBH27_001622"
     CDS             22311..24071
                     /locus_tag="QBH27_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDQ7013946.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            24196..24480
                     /gene="rplY"
                     /locus_tag="QBH27_001623"
     CDS             24196..24480
                     /gene="rplY"
                     /locus_tag="QBH27_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDQ7013947.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(24619..25626)
                     /gene="yejK"
                     /locus_tag="QBH27_001624"
     CDS             complement(24619..25626)
                     /gene="yejK"
                     /locus_tag="QBH27_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDQ7013948.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            25808..26035
                     /locus_tag="QBH27_001625"
     CDS             25808..26035
                     /locus_tag="QBH27_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDQ7013949.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            26055..27815
                     /gene="pbgA"
                     /locus_tag="QBH27_001626"
     CDS             26055..27815
                     /gene="pbgA"
                     /locus_tag="QBH27_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDQ7013950.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            27890..27966
                     /locus_tag="QBH27_001627"
     tRNA            27890..27966
                     /locus_tag="QBH27_001627"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27924..27926,aa:Pro,seq:ggg)
     gene            complement(28077..29020)
                     /locus_tag="QBH27_001628"
                     /pseudo
     CDS             complement(28077..29020)
                     /locus_tag="QBH27_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            29340..29987
                     /gene="narP"
                     /locus_tag="QBH27_001629"
     CDS             29340..29987
                     /gene="narP"
                     /locus_tag="QBH27_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDQ7013951.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(30022..31074)
                     /gene="ccmH"
                     /locus_tag="QBH27_001630"
     CDS             complement(30022..31074)
                     /gene="ccmH"
                     /locus_tag="QBH27_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDQ7013952.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(31071..31628)
                     /gene="dsbE"
                     /locus_tag="QBH27_001631"
     CDS             complement(31071..31628)
                     /gene="dsbE"
                     /locus_tag="QBH27_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDQ7013953.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(31625..33568)
                     /gene="ccmF"
                     /locus_tag="QBH27_001632"
     CDS             complement(31625..33568)
                     /gene="ccmF"
                     /locus_tag="QBH27_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDQ7013954.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(33565..34044)
                     /gene="ccmE"
                     /locus_tag="QBH27_001633"
     CDS             complement(33565..34044)
                     /gene="ccmE"
                     /locus_tag="QBH27_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDQ7013955.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(34041..34250)
                     /gene="ccmD"
                     /locus_tag="QBH27_001634"
     CDS             complement(34041..34250)
                     /gene="ccmD"
                     /locus_tag="QBH27_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDQ7013956.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(34247..34984)
                     /gene="ccmC"
                     /locus_tag="QBH27_001635"
     CDS             complement(34247..34984)
                     /gene="ccmC"
                     /locus_tag="QBH27_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDQ7013957.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSILGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(35026..35688)
                     /gene="ccmB"
                     /locus_tag="QBH27_001636"
     CDS             complement(35026..35688)
                     /gene="ccmB"
                     /locus_tag="QBH27_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDQ7013958.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(35685..36302)
                     /gene="ccmA"
                     /locus_tag="QBH27_001637"
     CDS             complement(35685..36302)
                     /gene="ccmA"
                     /locus_tag="QBH27_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDQ7013959.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(36321..36923)
                     /gene="napC"
                     /locus_tag="QBH27_001638"
     CDS             complement(36321..36923)
                     /gene="napC"
                     /locus_tag="QBH27_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDQ7013960.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(36933..37382)
                     /gene="napB"
                     /locus_tag="QBH27_001639"
     CDS             complement(36933..37382)
                     /gene="napB"
                     /locus_tag="QBH27_001639"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDQ7013961.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(37379..38242)
                     /gene="napH"
                     /locus_tag="QBH27_001640"
     CDS             complement(37379..38242)
                     /gene="napH"
                     /locus_tag="QBH27_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDQ7013962.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVIILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(38229..38924)
                     /gene="napG"
                     /locus_tag="QBH27_001641"
     CDS             complement(38229..38924)
                     /gene="napG"
                     /locus_tag="QBH27_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDQ7013963.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(38931..41417)
                     /gene="napA"
                     /locus_tag="QBH27_001642"
     CDS             complement(38931..41417)
                     /gene="napA"
                     /locus_tag="QBH27_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDQ7013964.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(41414..41677)
                     /gene="napD"
                     /locus_tag="QBH27_001643"
     CDS             complement(41414..41677)
                     /gene="napD"
                     /locus_tag="QBH27_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDQ7013965.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(41667..42161)
                     /gene="napF"
                     /locus_tag="QBH27_001644"
     CDS             complement(41667..42161)
                     /gene="napF"
                     /locus_tag="QBH27_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDQ7013966.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGSYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            42270..42434
                     /locus_tag="QBH27_001645"
     CDS             42270..42434
                     /locus_tag="QBH27_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013967.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            42569..43057
                     /gene="eco"
                     /locus_tag="QBH27_001646"
     CDS             42569..43057
                     /gene="eco"
                     /locus_tag="QBH27_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDQ7013968.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(43207..44853)
                     /gene="mqo"
                     /locus_tag="QBH27_001647"
     CDS             complement(43207..44853)
                     /gene="mqo"
                     /locus_tag="QBH27_001647"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDQ7013969.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEVLKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        10937 a        12181 c        11128 g        10776 t
ORIGIN      
        1 aattcagaca ttctgtgttc tccactaacg atgtaacatt ggcaattgat aattcagggt
       61 tgttggtata ttcacaggca agtgccattg acgaacatcc ctgcgcaaat cgaacagaat
      121 cgataaacgg cataccgtct acccaacagg aggccagccc cgccatcatg gcgtcgcctg
      181 ccccagtaac attgatgaca ttcgttttaa taggggccga ccacccactt tcaccgttga
      241 tatcactgta gtaaacgcca tcccctccca tgctcagcac caggcggttc aggccgtgtt
      301 gatgaaacca ggcggcgacc tttgcgacat cttcacgtcc ggaaagtgca attccgctca
      361 acgtttcggc ttcaagacgg ttaggtttta acgtatggat tttgctcaga tgatcgcgga
      421 ttttgacgca tttccatgcc gataccggat caataaatac aggcgtttcc ccgctatttt
      481 ccagaaccca gaccagcgcc tcttcactaa ggttgcaatc cgcgacaata acctttgcac
      541 cgcgaataaa ctcctgatgc tgggcaagaa attctgcaga aatacagtca ctaatactca
      601 tatcatttat cgcgacaagc atttctcctg tattatcgag cagtgataaa tagctggatg
      661 tattctcacc cggaacaatt aagcatttat cgacataaac accggattga ttagtttgcg
      721 ccagtaatga ttgcccataa aaatcaccac ctactgcact taaaagccag gcatttttcc
      781 ccagtaacgc cagattatga gcgatattcc ttcccacgcc acctggggta aatttaattt
      841 ttccgggatt cgaatccgca taattcaatg gatgatgtga atacccagcc acatcaatat
      901 tcgccgagcc aataattact atgtagtcct tttcgcgcat agcacgcccc ctggcaataa
      961 attgccaacg aagaaattgg gtaaaatagt cattacagtc aataagacta ttagagcatg
     1021 tgttcagatt taaacacatg ttcataataa gcatgtttaa cgtaatgtaa atcgatcaga
     1081 aaaaacgaaa tcggccagat atgaaggcgg tcacaaaatt tgtctattta tgcagcaaca
     1141 aaagagaaag ttgtgatgtg taacacacaa tatggcaggc aagaaaaagg gcagagatgt
     1201 ttctgccctc agattgttga caatgcgcgc tttgttcatg ctggatgtgg cgtaaacgcc
     1261 ttatccgacc tacaaaagcg caaaaattca atatattgca gggttcatgt aggtctgata
     1321 agcatagcgc atcaggcaat tttgcgtttg tcatcatcgg ggcagagatg tctcatcccc
     1381 gatgatgaag agaaattatg cgtttttttc tactatttgt gtttccgggc gtttgaggaa
     1441 ggcataggcc aaacccgcca ccagcgtacc ggcaataatt gctaccaggt aacccaatac
     1501 cggcgtaata gcgccaggga tcagcagaac aaacagacca ccgtgcggag ccatcagttt
     1561 cgcaccaatc gccattgaga ttgcgccagt cagcgcccca ccaacgatac agcacggtag
     1621 cacacgcatc ggatcacgag cagcaaacgg aattgcacct tccgaaataa agcacagacc
     1681 aagtaccaga gcggctttgc caccttcctg ctgcgcttta tcgaatttac gacgcgccac
     1741 cattgttgcc agacccattg ccagcggtgg caccatacct gccgccataa tcgccgccat
     1801 cgggccatag gtttgagtac tcagcagacc cacaccgaat gcgtacgctg ctttgtttac
     1861 cggaccgccc atgtcagtac acatcatgcc accgaggatc gcccccagca gaaccgcatt
     1921 cgcagtcccc atggtctgca gccagtgagt caacccatcg agaatgccag caaccggttt
     1981 accgattagg tagatcatcg ccagaccgac caccagactg gaaattagcg gaatgatcag
     2041 gatcggtttc agtgcctcca tactctgtgg cagtttcagt tgcgtactga ttaacttcgc
     2101 aatgtaaccc gccaggaagc ccgcaataat accgccaatg aagccagaac cggtgctaac
     2161 cgccagcata ccgccaatca gacccggcgt gagacccgga cgatcggcaa tggagaaggc
     2221 aatataacct gccagtaccg gcaccatcag cgcaaaggct gaaccaccac caatctgcat
     2281 cagcgccgct gccaacgtac ccggctcttt aaacgcttcg ataccaaaag caaaagaaag
     2341 cgcgatacac agaccacctg caacaaccat cggcagcatg taagagacgc ccgtcagcaa
     2401 gtgacggtaa gcgcctgcac tctctttctt accttcagtg gtcgctgttt gagctttgcc
     2461 cgctggttca tacggcgttg cttcagcaac cgctttatcc agttcctgcg cagttttctt
     2521 cagcgccaga ccggtagagg tgcgatacat cggtttacca gcaaatttcg ccagatccac
     2581 ttcgatatct gccgccacaa tcaccagatc cgctgctgcg acttcttcag gtgtgattgc
     2641 attacccgcg ccaacagaac cacgggtttc aactttcacc caccagccac gttttttcgc
     2701 ttcggtttca atggcttcag ccgccataaa ggtgtgtgct acaccagtcg ggcaagcagt
     2761 caccgcaact acgcgtttcg gaccgctagc agcaaccggt gctgtcgcag cgaccggcgc
     2821 agtgtaaggt ttcgcatggc ctttggcttc actcaggaac agctcagggt gcgcaactgc
     2881 ccgggaaata tcgcccagcc agacattttt accgttcagc gcgctgtcat tcgggatgaa
     2941 atcaccgaga acaatcgcca tttctgcgtc gttcggattg tcgatgattt ccagttttgc
     3001 ttttcgcgcc gccgcgccca gcagggtctt cgccatatag gcgcgtgcct gaccgagatt
     3061 agcgtcaata atcagcagcg ttttcattat gcctctcctg ctgtcagtta aaaggttgta
     3121 agtcgacgcg cgccatcatt gcggccaact gcggacgatc ggtaataccc acattgcttt
     3181 gacttaccgc cagggctgca acagctgtcg ccagacgcag tgtgtgttca ctggattcac
     3241 gcatcagcaa gccataaatc aggccaccaa ccatagaatc ccctgcgcca acggtgctta
     3301 cgacatcgac tgacggtggt ttggcgatcc attcgccgga ggcattaacc caaagcgcgc
     3361 cttcggcacc cagtgaaata acaacatgcg caataccttg ttcacgtagc gcatgtgcag
     3421 cttcaatcac atctttcatt tcaggcagtt tacggcctgc ccagatttcc agctcgcggc
     3481 ggttaggttt caccagccac ggtgccgctt tcaaacctgc tactaacgct tcacggctac
     3541 tatcaaagat aatgcaagga cactgactac gcaggcgagt catccagtcg gtgaacgctt
     3601 ccgggctgac gcctgacggt aagcttccgc tgacacagac catatcgaac tgaccgagcc
     3661 agctcagaga atcagtcaca aagcgttccc agtcggcggg ggtaacttca aaacccgaga
     3721 agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt aacgttaatt cgggtgcgcc
     3781 cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct gaacagttgc tgaaaaccat
     3841 cctgattgtc tttacccagg aagccgccaa cggtgacatc aattcccagg tcttttaata
     3901 ctttggccac gttgatgcct ttacccgccg catgcagacc ggtggttttc accaggttca
     3961 cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc ataagccgga ttaagggtga
     4021 tcgtagcaac acgtctgctc attatgcgcc ctccccaaga ccagcagcga tagcgtcgcc
     4081 aatggctttc agcgcctgtt cagcatctgc accctgggcg gtaaagcgta ggcgatgacc
     4141 tttcttaacg ccaagtgcca caactttcat cagactacgt ccgtttgccg gtttgccggt
     4201 gccatcaagg tttgtcacgg taatatcact gttaaattgt ttaatggtat tgaccagcat
     4261 ggtacctgga cgagcatgca ggccgtgttc attgcgcacc acaaactccg cgcttaacac
     4321 gtcgtcggtc ggcgcatcat cgctggtcag cagcgccagc aacgttgccg catccgcttt
     4381 cagcaagcgg tcagctttat tatcgagcaa caaatcagcg agacgcttaa gaaccgcgat
     4441 gggctgatcg tcattcattg ccacactcac cagcatggct gccgtttcgc cgtccacatc
     4501 aaaagcattt gccgcacggc ttaccgcaat cgcgctacgc agattgcctt cggcgctatc
     4561 gctcagccag ataccctgtc cgaggttcag cggttgttca ttgatggctt tggtgacgaa
     4621 agtggcgtca actgcccccg cttctttcag acgcgcagcg ttcagcgcct gaagagtcag
     4681 cagatcgctg gcgacgatat ccagcgtcag catttcgttg tcgagcttca gctgctcact
     4741 ctgcttttcg cccatcagca atgcgcgaag ttcttctgct gttgttgctg acttcagttg
     4801 ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg cgtagcaggc ccagatgttc
     4861 atccgagctg gcagcaatac cgatcgccac gtacgctacc tggccgtccc cccaggtgac
     4921 gccttccggg aactgaaata cctgaacgcc ggttttcagc acctgatcgc gggtgtcggt
     4981 agtgccgtgt ggaatagcaa taccattgcc gaggaacgtt gaggtttgct gttcgcgcgc
     5041 cagcatgcca ttgacgtagc cttcagctac attaccggcc tgcaccagcg ccgcagcgac
     5101 ctggcgaatc gcctcttctt tgtctccggc cttttcgccc ggatggatgt cctgtacaga
     5161 taactggaac atagttctcc tctcttgctg aattgaaacg attcagcttc tatgagaaaa
     5221 aaagcgccaa cctggcttag ggttaaagac aagatcgcgc tgaaacgttt caaggaagca
     5281 taatacttct gtttcagcgc gcaaggaaag ccgtaaagtt atgagcaaaa gtttgatgtg
     5341 ctgcacaatt ttttgtcatt tttcctaatt gctgatggga aaatccgtta tcagcaattt
     5401 catttcagca gtgtcagcag ccaatcagca acagtgaaat ggcattttga ttattcgtgg
     5461 cgcttttgat ttcgctgtct gtttattttc tgacaagcag cgtaaactcc gcgtcttcct
     5521 cttccagtga tcgaccagca tgcataactc ccccacagtc accagcgcga aatcgtttga
     5581 cctgacctcg acggcgtttt taatcgttgc ctttctcacc ggtattgcgg gcgctctgca
     5641 aaccccgaca ctcagtattt ttcttactga tgaagtacat gcccgtccgg cgatggtggg
     5701 attcttcttt accggcagcg ctgtcattgg gattctggtg agtcagtttc tcgccgggcg
     5761 ctctgataag cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt taggcgtgct
     5821 ggcctgcacc ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg ttggcgtctt
     5881 tcttagcagc tttggctcga ccgctaaccc gcaaatgttt gcccttgccc gtgaacatgc
     5941 tgacaaaacc ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc aggtttcact
     6001 ggcatgggtc attggcccac cgctggctta tgccttagcg atgggtttca gctttacggt
     6061 aatgtatctg agcgcagcgg tagcgtttat tgtttgcggc gtgatggtgt ggctgttttt
     6121 gccgtcgatg caaaaagagc ttccgctggc gaccggcacg gttgaagcgc cgcgccgtaa
     6181 ccgtcgcgat acgttgctgc tgtttgtcat ttgtacattg atgtggggct cgaacagcct
     6241 gtacatcatc aacatgccgc tatttattat caacgaactg catcttcccg agaaactggc
     6301 cggtgtgatg atggggaccg ccgccgggct ggaaatcccg accatgttga tcgccggata
     6361 ttttgccaaa cgtctgggta agcgcttctt aatgcgcgtt gctgccgtgg gtggcgtctg
     6421 tttttacgca ggaatgctga tggcgcattc acctgtcatt ctgttgggct tgcagctgct
     6481 aaatgctatt tttattggca ttcttggcgg catcgggatg ctctattttc aggatttgat
     6541 gcccggtcag gcaggttcag ccaccacgct ctataccaac acttcgcgcg tgggctggat
     6601 catcgcagga tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg ctgtgttctg
     6661 gtttgcgatg gtgatgatta tcgccactct gttttgctta ctgcgaatta aagatgttta
     6721 aggcgcggtc aacatttcca gatcgataag ccagatcatg gcctgctgac gcgagttacc
     6781 gcacatttca gcgctggctt gtaagcctgc acagactttc ggtcgcagtg gcgaggtgaa
     6841 aattttacag cgttgctgtt catcaagctg aatgcaggga gtattggcgg gcttgccatc
     6901 tggcataccg ggaatagggc tggaaattga aggggcagtg caacacgccc cgcaacccgg
     6961 acggcattcc atacgttgtt ctcttaaggt taaccaccgg attgatgagc gcgcacagta
     7021 ccaccttttt tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc cgtcgcgagt
     7081 actttgtcgc gatatttttg acatttttga ctacaggaat ttttcgatgc caagagcgaa
     7141 cgaaattaaa aaaggtatgg tactgaatta caacggcaaa ctgctgctgg ttaaggacat
     7201 tgatattcag tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc gtttttctga
     7261 tgtccgtacc gggctgaaag tagaagagcg tttcaaaggt gatgatatcg ttgacaccgt
     7321 gacgctgacc cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat atgtctttat
     7381 ggataaagaa gactataccc cgtatacctt caccaaagat cagattgaag aagagttgct
     7441 gtttatgcct gagggcggca tgccggacat gcaggtgctg acctgggacg gtcagttgct
     7501 ggcgcttgag ctgccgcaga ccgttgatct ggaaatcgtt gaaactgcac caggtatcaa
     7561 aggggcatct gccagcgccc gtaacaaacc ggcgacattg agcactggtc tggtgattca
     7621 ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc catatcgaag aacgccgtta
     7681 tatgggtcgt gctgactaac ttcagccgca tgcagaaaag ggatagctca ggctgtccct
     7741 tttttaattt attataccaa tcttctattt tgcgcttcct gaacacctta cgcccgcaat
     7801 tttcactcgt tgattgatcg ccctcacact tcatcgcatt aacaatccag accaatttca
     7861 attgctgtca tataacttta cactgtcatt gttaattaat cgttactaag acgtgactcc
     7921 tatgaataca atcgcctccg ttacgctccc gcatcatgta cacgctccac gctacgatcg
     7981 acagcagttg caatcacgta tcgttcattt tggctttgga gcctttcacc gcgcacatca
     8041 ggcattactg accgatcgtg tgctgaatgc ccagggcggc gactggggga tctgtgaaat
     8101 cagcttgttc agcggcgatc aactgatgag ccagctccgc gcacagaacc atttatatac
     8161 cgtgctggag aaaggtgcgg acggcaatca ggcgataatt gtcggtgccg ttcacgaatg
     8221 tctgaatgca aaactggatt ccttagcggc aattattgag aaattttgcg agccacaggt
     8281 ggcaattgtt tccctgacaa ttaccgaaaa aggctattgt attgacccgg ccaccggtgc
     8341 actcgacacc agcaatccgc gtattattca cgatctacaa aaccctgaag aacctcactc
     8401 cgcgccaggt attctcgtcg aagcactgaa acgccgccgt gagcgcggcc atacaccgtt
     8461 taccgtgctc tcctgcgaca atattcccga caatggccat gtggtgaaaa acgcggtgct
     8521 gggaatggca gaaaaacgtt cgccagaact cgccgggtgg ataaaagaac acgtcagttt
     8581 tccgggaacc atggtcgacc gcattgttcc ggctgcaaca aacgaatcac tggcggaaat
     8641 aagccagcat ctgggggtga atgatccctg cgcgattagc tgcgaaccgt ttatccagtg
     8701 ggtggttgaa gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag gtgtacaaat
     8761 ggtgaatgat gtcctgccat gggaagagat gaaactgcgg atgcttaatg gcagccactc
     8821 ttttctcgct tatctgggtt acctctcagg attcgcccat atcagtgatt gcatgcagga
     8881 tcgcgcattt cgccatgccg ccagaacatt aatgctggat gagcaagcgc cgacactgcg
     8941 gattaaagat gtcgatttaa cacaatatgc ggataagtta attgcacgtt ttgctaatcc
     9001 ggcactgaaa cataagacct ggcaaatcgc gatggatggc agccagaaat taccgcaacg
     9061 catgttggca ggtattcgca tacatctggg gcgcgaaacg gactggtcgt tgctggcatt
     9121 aggcgttgca ggctggatgc gttacgtcag cggcgttgat gatgccggaa atgccattga
     9181 tgttcgcgat ccgcttagcg ataaaattcg tgaacttgtt gcaggcagca gcagtgaaca
     9241 acgcgtaacc gccctgcttt ccctgcgtga aattttcggt gatgatctgc cagataaccc
     9301 gcattttgtg caggtcatcg aacaagcctg gcaacaaatc gcacaattcg gcgcgcatca
     9361 ggcgctatta aacaccctca aaatttaacg atttctgcgg ttaaagcgga tgaagctcgc
     9421 cttcgtccgc tctccccttc tcttttctgc cttttttagc caggattaac gctcagttaa
     9481 cttaccagag tattgcggag ccgttgtgac cagaaccaac ctcatcaccg gttttctcgg
     9541 cagcgggaaa actacgtcga ttcttcatct gttagcccat aaagatccca acgaaaaatg
     9601 ggcggtactg gttaatgaat ttggggaagt cggaattgat ggtgctttgc tcgccgatag
     9661 cggcgcactg ctgaaagaga tccccggcgg ttgcatgtgc tgcgttaatg gtttacccat
     9721 gcaggtaggg ttgaatacct tactgcgtca gggaaaacca gaccgcttgt tgatagagcc
     9781 gaccgggctg ggccatccga aacagatcct cgatctctta accgcgccag tctatgaacc
     9841 gtggatagat ctgcgcgcca cgttgtgcat tctcgatcca cgcctgctgc tggacgaaaa
     9901 aagcgccagc aatgaaaact tccgtgacca gctggctgcc gcagacatta ttgtcgccaa
     9961 taaatccgac cgtgcgacgc ccgaaagtga gcaagcgcta gagcgctggt ggcagcaaaa
    10021 tggtggcgat cggcaattaa ttcacagcga gcatggaaaa attgacggtc atcttctgga
    10081 tttgccgcgt cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc atcagcatag
    10141 cgtgaaaaaa gggttagcag cgttaagcct gccagagcat caacgctggc gtcgcagtct
    10201 gaacagcgga caagggcatc aggcctgtgg ctggatattc gacgctgata cggtattcga
    10261 caccattggt attctggaat gggcgcgact tgcaccggtg gaacgcgtca aaggcgtgct
    10321 gcgtattccc gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc acattgaaac
    10381 gcaaaacgtt gcgccaccgg acagtcgtat tgagctgatt tccagcagcg aagctgactg
    10441 gaatgccttg cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt aaggttgcct
    10501 gcgtttttca gtaagataat tagagaaaat atgattaaaa atttgccgca aatagtgttg
    10561 ttgaatattg tcggcctcgc gctgtttctt tcctggtata ttcccattaa tcatggattc
    10621 tggttgccga ttgatgcgga tattttttat ttctttaatc agaaactggt cgaaagtaag
    10681 gcctttttgt ggctggttgc attgaccaac aatcgcgctt tcgacggttg ttcactgctg
    10741 gcgatgggaa tgttgatgct gagtttctgg ctgaaagaaa acgccccagg cagacgacgt
    10801 atcgtgatta ttggtctggt catgctatta actgcagtgg tattaaacca gctgggtcag
    10861 gcattaattc ctgtaaaacg ggccagtcca acgttgactt ttaccaatat taaccgcgtc
    10921 agcgaactgc tctctgttcc cacgaaagat gcctcacgag atagctttcc cggcgatcac
    10981 ggtatgatgc tgcttatttt ttcggcattc atgtggcgtt atttcggcaa agttgctggc
    11041 cttatcgccc ttattatttt tgtggttttt gcgtttccca gagtaatgat tggcgcacac
    11101 tggtttactg acatcatcgt cggttccatg accgtgatat tgatcggttt gccctgggtg
    11161 ttgctgacgc cattaagtga tcgattaatc accttttttg acaaatcact acctggaaaa
    11221 aacaaacatt tccaaaacaa ataactcaca gtaattaaca tcatcaggtt tatttttata
    11281 gtgaggataa tcctgatgat gcgcctcctg ctttcatcca tcgagcgcaa aaatcattct
    11341 ctaagtaaat gaatggattg catgcgtttc actcaatcgt actttaattg accaatcccg
    11401 ctgattaact ttctgtatca ctttttctta taaaaaatca tgtaaaaccg ctcgccaaga
    11461 ccgcaccaat cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca
    11521 tataattttg tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg
    11581 taattagtct cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa
    11641 gggaaaacaa acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatc
    11701 cccgcgattg cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat
    11761 atgcatcctg agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat
    11821 gaatttgaaa acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct
    11881 gactggaaag gcgtacgcta tcgtctgggc ggcagcacta aaaaaggtat cgattgttct
    11941 ggtttcgtac agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttat
    12001 gaacagcagg aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg tgatttagtt
    12061 ctgttccgtg ccggttcaac gggacgccat gtcggtattt atatcggcaa caaccagttt
    12121 gtccatgctt ccaccagcag tggtgttatt atttccagca tgaatgaacc atactggaag
    12181 aagcgttaca acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa
    12241 tcccttggct atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt
    12301 cattcatcag agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt
    12361 atcttttaaa tgttgtcgta atttcaggaa attaacggaa tcatgtttat acgcgctccc
    12421 aattctggac gtaagctcct gcttacctgc attgttgcag gcgtgatgat tgcgatactg
    12481 gtgagctgcc ttcagttttt agtggcctgg cataagcacg aagtcaaata cgacacactg
    12541 attaccgacg tacaaaagta tctcgatacc tattttgccg accttaaatc cactactgac
    12601 cggctccagc cgctgacctt agatacctgc cagcaggcta accccgaact gaccgcccgc
    12661 gcggcgttta gcatgaatgt tcgtacgttt gtgctggtaa aagataaaaa aacattctgt
    12721 tcatctgcaa ctggtgagat ggacattcca ctcaatgaat tgattccggc gctcgacatt
    12781 aataaaaacg tcgatatggc gatcttaccc ggcacgccga tggtgccgaa caaacccgca
    12841 atcgtcatct ggtatcgcaa ccctttgctg aaaaatagcg gcgtgtttgc cgctctgaat
    12901 ctcaacctga cgccttcact cttttatagt tcacggcagg aagattacga tggcctcgcc
    12961 ctcattattg gtaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaatgaa
    13021 ttaaccgaca tgccggtccg tgaaactaaa attgcgggca ttcctctgac cgttcggctt
    13081 tatgcagatg actggacatg gaacgatgtg tggtacgcat ttttactggg tggcatgagt
    13141 ggaactttcg ttggacttct ctgctattac ctgatgagtg tgcgtatgcg cccaggcaga
    13201 gaaatcatga ccgccatcaa gcgcgaacaa ttttacgtgg tatatcaacc ggtggttgat
    13261 acacaagctt tgcgggtaac gggcctggaa gtactgctac gctggcggca tcctgtagca
    13321 ggagaaatac ccccggatgc cttcattaac tttgccgaag cgcaaaagat gattgtgcca
    13381 ctgactcagc acctgtttga gttgattgcc cgcgatgccg cagaattaga aaaagtactg
    13441 ccggtaggcg tcaaatttgg cattaacatt gcgccggccc acttgcacag cgaaagcttt
    13501 aaagcggata tccagaaact gctcacttcc ctgcccgcac accatttcca gattgtgctg
    13561 gaaattaccg agcgcgatat gctgaaagag cgagaagcca cacaactctt cgcctggctg
    13621 cattcggtcg gcgtagaaat cgctattgat gacttcggca ccgggcacag cgcgcttatc
    13681 tatcttgagc gttttacgct cgattatctg aaaattgatc gtggatttat caacgccatc
    13741 ggtacggaaa cgatcacttc acccgtactt gacgcggtgc tgacgctggc gaaacgtctc
    13801 aatatgctga cagttgctga aggggtcgaa acgccagaac aggcacgatg gctaagcgaa
    13861 cgcggcgtta atttcatgca aggctactgg attagtcgcc cgttaccgct ggacgatttt
    13921 gttcgctggc tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac gtcccttatt
    13981 attcatagtg aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag
    14041 atgattgtgc gcatactgct gctgtttatc gctctgttca cctttggtgc gcaggcgcag
    14101 gctatcaagg aaagctatgc ctttgcagta ctgggcgaac cccggtacgc atttaatttc
    14161 aaccattttg attatgtgaa ccccgccgcg ccaaaaggtg ggcaaataac gttgtctgcc
    14221 ctcggcacct tcgataattt caaccgctat gcactgcgcg gcaatccggg cgcacgcacc
    14281 gagcagctgt acgacacgct atttacgact tccgatgacg aaccaggcag ttattacccg
    14341 ctgattgctg aaagcgcacg ctatgctgac gattattcct gggtggaggt cgctattaat
    14401 ccacgcgccc gttttcatga tggttcgccc attactgccc gcgatgtaga gtttactttt
    14461 caaaaattta tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc
    14521 aaagccattg cgccgttaac cgtgcgcatt gagttagcta aacccggcaa agaagatatg
    14581 ctgagtctgt tttcgctgcc ggtatttcca gaaaagtact ggaaagatca caaaattagc
    14641 gacccgctcg ccacgcctcc gcttgccagt ggtccgtacc gcattacgtc ctggaaaatg
    14701 gggcaaaata ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac
    14761 cgtggacgct ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc
    14821 tttgaagcgt ttaaagcagg agcctttgat ttgcgtatgg aaaacgacgc caaaaactgg
    14881 gccacgcgct ataccggtaa aaatttcgat aaaaaataca tcatcaaaga tgagcaaaag
    14941 aacgaatcag cccaggatac gcgctggctg gcgtttaata tccaacgtcc ggtattcagc
    15001 gatcgccggg tgcgggaagc gatcactctc gcctttgact ttgaatggat gaacaaggcg
    15061 ttgttttaca atacctggag tcgcacgaac agttattttc agaataccga atatgcggcc
    15121 agaaattacc ccgacgccgc ggaactggtg cttctggcac caatgaaaaa agatctaccg
    15181 ccagaagtct tcacgcaaat ctaccagccg ccggtatcca aaggcgatgg ctacgatcgt
    15241 gacaacctgt taaaagccga caaacttctc aacgaagcag gctgggtgct gaagggtcag
    15301 caacgcgtta atgccacaac aggtcagcca ctcagctttg aattattgct tcccgcaagc
    15361 agcaatagtc agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaacatg
    15421 gacattcgca aggtggataa ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac
    15481 atgatgccgc gcgtatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg
    15541 tcatcggaat atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac
    15601 tcgctgatca accaaattat tgccgcgcag ggaaataaag aaaaattact gccgttggga
    15661 cgagcactgg atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg
    15721 gaagaccgtc tcgcctggtg ggataaattc tcccaacccg ctgtacgccc agtttacagc
    15781 ctgggtattg atacctggtg gtatgacgtt aataaagcga cgaaactgcc gtcagccaga
    15841 caacagggag agtagatggg cgcttatctg atccgccgtc tgttgctggt gatcccaacg
    15901 ctatgggcga ttatcactat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc
    15961 gaccaggcca tcgccgccat tgagtttggc aatgccggag tattacccgg cgcaggcggt
    16021 gaaggtgttc gcgccagcca tgcgcaaacg ggcgtcggca atatcagcga cagtaattac
    16081 cgtggcggac gcggattaga tccagaagtg atcgccgaga tcactcatcg ctacggtttc
    16141 gataagccga tccacgaacg ttacttcaaa atgctctggg actacatccg ttttgatttt
    16201 ggcgacagcc tttttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtc
    16261 tccatcaccc tcggattgtg gagcacgctg attatctatc tggtgtcgat tccgttaggc
    16321 attcgcaaag ctgtttataa tgggagccgt tttgacgtct ggagtagtgc atttatcatc
    16381 atcggctacg ccattccggc ctttttgttt gccatcctgc tgattgtctt cttcgcgggc
    16441 ggcagctatt tcgatctgtt ccctctgcgc ggcctggttt ccgctaactt tgattcgctg
    16501 ccgtggtatc agaaaatcac cgattatctg tggcatatca cgctgccggt gctggcgaca
    16561 gtgattggcg gctttgcggc tctgaccatg ctgacaaaaa actcattcct tgatgaagtg
    16621 cgtaagcaat acgtggtgac ggcacgtgcg aaaggggtaa gtgaaaaaaa tattctctgg
    16681 aaacatgtgt tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc
    16741 agcatgtttt ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggtcttggc
    16801 ttactgggct acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat
    16861 attttcaccc tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctggtc
    16921 gatccgcgta tagactttga gggacgttaa tgtcgcgact cagccccgtc aatcaggccc
    16981 gttgggcgcg ttttcgccat aaccgtcgcg gctactggtc gttatggatt ttcctcctct
    17041 tgtttggttt gagtttgtgt tctgaactta tcgccaacga taaaccgttg ctggtgcgtt
    17101 atgacggcag ttggtatttc ccgttgttga aaaactacag cgaaagcgat tttggcggcc
    17161 cgctggcaag tcaggctgat tatcaggacc cgtggctgaa acaacgactg gaaaataacg
    17221 gctgggtact gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata
    17281 agcccttccc ttctccaccc tcccggcaaa actggctggg aacggatgcc aatggcggcg
    17341 atgtgctggc gcgcattctc tatggcacgc ggatctcagt tctgtttggc ctgatgctga
    17401 ccctctgctc cagcgtgatg ggtgtgctgg cgggggcgct acaaggctat tacggtggta
    17461 aagtcgatct ctgggggcaa cgttttattg aagtatggtc ggggatgccg acgctgtttt
    17521 tgattatttt actttccagc gtcgtacagc ctaacttctg gtggctgctg gcaattactg
    17581 tcttgtttgg ctggatgagt ctggtcggcg tggtgcgggc ggagttttta cgtacccgta
    17641 atttcgacta catccgcgcg gcgcaggcgc ttggcgtcag cgatcgcagt atcatcctgc
    17701 gtcatatgtt gccaaatgcc atggtcgcta ccctcacttt tttaccgttt attttatgta
    17761 gttcgataac caccctgacc tcactcgatt tcctcggctt cggtctgccg ctcggttcac
    17821 cgtcactcgg cgaactcctg ttacaaggga aaaataacct tcaggccccg tggcttggga
    17881 tcaccgcctt cttatcggtg gcgatattgt tgtctttgct gatctttatt ggtgaagccg
    17941 tccgcgacgc atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt
    18001 gaaaatttgt cggtgggttt tcgccatcag caaactgttc gtaccgtagt gaatgatgtg
    18061 tcactacaca ttgaggctgg cgaaacgctg gcgctggtag gtgagtctgg ttcaggtaag
    18121 agcgttaccg cgctgtcaat tttacgcctg ctcccttgcc cgccggttga atatctctcc
    18181 ggcgatattc gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac gttgcgcggt
    18241 gtacgcggta ataagatcgc catgattttt caggagccga tggtgtcgtt aaatccatta
    18301 cataccctgg aaaaacagct ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa
    18361 gcggctcgtg gcgaaattct taactgcctt gatcgcgtcg gtatccgcca ggcggcaaaa
    18421 cggctgacag attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg
    18481 atggcgctgt taacgcgacc ggaattatta attgccgatg aaccaaccac cgcgctggac
    18541 gtctctgtcc aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg
    18601 ggtatgctgt ttattactca taacctcagc attgttagaa aactggccca ccgcgtggcg
    18661 gtaatgcaaa acggtcgctg tgtcgagaaa aataacgccg ctacgctatt tgcctcaccc
    18721 actcatcctt acacacaaaa gctactcaac agtgaaccat ctggcgaccc ggtgccattg
    18781 ccagaacctg cctcaacgtt gctggatgtt gaacaacttc aggttgcctt ccccattcgc
    18841 aaagggattt tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg
    18901 ctacgggcgg gtgaaacact gggtttagtg ggcgagtccg gttccgggaa aagcacgacg
    18961 ggactggcgc tgctgcgact gattaactct catggcagca tcgtctttga cggtcagcca
    19021 ctgcaaaatt taaatcgccg ccagctgtta cctattcgtc atcgcattca ggtggtattt
    19081 caggatccaa actcctcact caacccacga ctcaacgttt tgcaaattat tgaggaaggc
    19141 ttacgggttc accagccgac gctttctgcc gcacaacgcg aacaacaagt gatagccgtg
    19201 atgcatgaag tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt
    19261 ggtcagcggc aacgtattgc gattgccagg gcgttaattc ttaagccctc gctgatcatt
    19321 cttgatgaac caacatcatc actcgacaaa acggtgcagg cgcaaatatt gacgctattg
    19381 aaatcattgc aacaaaagca tcaactggcc tatttgttta tcagccacga tttgcacgtt
    19441 gtccgcgcgt tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga
    19501 ccgtgcgcgc gcgtgtttgc cgcaccgcag caggagtata cgcgtcagct actggcgttg
    19561 agctgacgct taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt
    19621 tttcaatcgg catgttgcag caaattcgtc ctggcgattt acaaacaggc acggctcacc
    19681 ttcacactcc agcaccgaac atggaacttc gatgtcgctt aacgcctggc ttagtttgta
    19741 catcaccgac catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt
    19801 gtcaccgcac ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga
    19861 ccagcgatag ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt
    19921 tccccggaag tacattttac ttcacaattt atttacatca attttaacac atcatcaaca
    19981 atccgtcttt atacagatcg taaagattga tgcctcgcct gcgtggccta tatggctctg
    20041 atttaaataa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc cgtttctgcg
    20101 atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg attttacata
    20161 aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa caaaggaagc
    20221 cttttagctt cctcgttgtg caatagatca ccgttttttc ggccgactgg cgtacagaca
    20281 gaagagaatg gagctggttg cacagaatgc aattgaccaa atcatcggcc atgcagagtt
    20341 aaaggtcgca agagaaagta atgcgccaac aattgcccct atgccgaaac ggaaggttcc
    20401 tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga ccgccatcgc
    20461 attggatgac accatcgaca cgcagcccac aaacgccgca acgccaacca ccagcgacca
    20521 gaatcccagc cccagcagtg cactgatgac catccacgct gccataataa actgtatcca
    20581 caaccccgag cggaacatat ttaacgcgcc aatacggcgg acgaagcggc tgttaaagat
    20641 ggtcatcacg aacagaaaaa gaatgtttag cgcaaagtaa taaccaaagt tttccggcgc
    20701 gacgtggttg atttcaatgt agacaaacgg tccggcgctt aagaatgaga acatcccggc
    20761 aaagctgaaa ccgctggcca gcatataact caggacacgc ttatgacgga acagcgccgc
    20821 aaagttacca atagtggtac gaatgtgaaa aggctggcga cgctccggtg gtaaggtttc
    20881 tttgatcaga aagaagatca ttgccgaagc cagaatcgcc gccacggcga ggatccagaa
    20941 gatgtaatgc cagctcagcc acaccagcac ccagccgcca actatcggtg ccatcagcgg
    21001 tgcaatggtc gtcaccagca tgacaaacga catcatccgt gagaactctt ctttcgggta
    21061 gatatcacgc atcaaagcgt tgataaccac gctggccgca gccgcagcca gcccgtggaa
    21121 gaaacgcatc acaatcagct gatcgatggt ttgtgccaac gcacacgcca ccgcggcggc
    21181 ggcaaacacc agcgtaccgc caagaaccac cggcttacgc ccgaagctgt ctgccatcgg
    21241 cccgtagatt aactgcccca acgcaaagcc cagaatataa gtactgaggg tcatctgcgt
    21301 actgcccgcc ggtacaccaa actgcgctga aattaccggt agcgcaggca gatacatatc
    21361 aatcgacagc ggcatcaaca tggccagcag gccaaggata aaaacaatag caaacgacga
    21421 atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg aaaagttaga cgacgctggc
    21481 aatttcttct tcagttaacg gacgatattc accgggggct aaatcagcat ccagcgtaat
    21541 accgccaata cgttcacgat gcagctcaac cacatggtta cccacggcgg cgaacatacg
    21601 tttcacctga tgataacgcc cttcgctgat ggtcaaacga acctgcgttg gggtaatcac
    21661 ttccagcacc gccggcttag tgagatcttt ttcgttatgc agctgcacgc ctttagcaaa
    21721 ttgctctgcc gtatcgtcag ctacgggtga ttccagcgtc accagatagg tcttctcgca
    21781 atgatggcgc ggggaagtaa tgcggtgcga ccactgacca tcatctgtca ttagtaccag
    21841 accggtggta tcgatatcca gacgccctgc agcgtgcagt ttccacgcta ccggttcatc
    21901 aagaaaatag agcaccgttg ggtgatccgg gtcgtccgtg gagcaaacat agccctgagg
    21961 cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc agcgggttgc catcgtaagc
    22021 gacatcatgt tcagggagca gtttgaacgc tgcattacgg acgatttcgc catcgacggt
    22081 gacacgattg ccgcggattt cacgcccggc aatagcacgg ctaacgccga gttgctgtgc
    22141 gataaattta tcaagtcgca tgtgtgtgat tttgcctgta aaaaacggag gtcgggtaat
    22201 gacccgaaaa tctgaactgt tgtctgccca gtatagcggt ctaataactt ccctcaaggg
    22261 aaaaagatcc gtggcatact attagcagaa taatctacct acgcgagacc atgattttta
    22321 cactccgccc atatcagcaa gaagccgtgg atgccacgct caaccatttt cgtcgtcata
    22381 aaacccctgc cgtaatcgtg ctgcccaccg gtgcaggtaa aagcctggtg atagcggaac
    22441 tggcgcggct ggcgcgtggt cgcgtgctgg tgctggcaca cgttaaagaa ctggtggcac
    22501 aaaaccatgc aaaatatcag gcgctggggc tggaagccga tatttttgcc gccgggctaa
    22561 agcgcaaaga gagccacggt aaagtggtat ttggcagcgt gcagtcggtc gcccgtaatc
    22621 ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga tgaatgccac cgtattggtg
    22681 acgatgaaga gagccaatat cagcaaatcc tcactcacct gaccaaagtg aatccccact
    22741 tacgcctgct ggggctgact gccacgcctt ttcgactggg caaaggctgg atctaccagt
    22801 tccattatca cggcatggta cgcggcgatg agaaagccct tttccgtgac tgcatttatg
    22861 agctgccgct gcgttatatg attaaacacg gctatctgac gccgccagaa cggctggata
    22921 tgccagtagt gcaatacgat ttcagccgct tgcaggcaca gagtaacgga ctgttcagcg
    22981 aagccgatct caaccgcgag ctgaaaaaac agcaacgcat tacgccacac atcatcagcc
    23041 aaatcatgga gtttgctgca acgcgcaaag gggtgatgat tttcgccgcg acggtcgaac
    23101 acgcaaaaga gatagtggga ttactgcctg ccgaagatgc agcactgatt actggcgaca
    23161 cacccggctc tgagcgcgat gtgttaattg atgattttaa agcccagcgt tttcgctatc
    23221 tggtcaacgt cgcggtactg accaccggat ttgacgcccc gcacgtcgat cttatcgcca
    23281 ttctgcgccc taccgaatcg gtgagtcttt accaacaaat tgtcgggcgc ggtctgcgcc
    23341 tggcccccgg caagactgat tgcttaattc ttgattatgc gggtaaccct cacgatctct
    23401 acgcgccgga agttggtaca ccgaaaggca aaagtgacaa cgttccggta caggttttct
    23461 gccctgcctg cggttttgcc aacacctttt gggggaaaac gaccgccgac gggacattga
    23521 ttgaacactt tggtcgtcgc tgtcagggat ggtttgaaga tgacgacgga catcgcgaac
    23581 agtgtgactt ccgtttccgt tttaaaaatt gcccgcaatg taacgctgaa aatgatattg
    23641 ccgcccgccg ttgccgcgag tgtgacacca ttctggttga cccggatgat atgttaaaag
    23701 cggcgctacg actgaaagac gcgctggtat tacgctgtag cggcatgtct ttgcagcatg
    23761 ggcacgacga aaaaggcgaa tggttgaaaa tcacctatta cgatgaagac ggcgcggatg
    23821 tgagtgagcg tttccgtctg caaacgcccg cccagcgaac tgccttcgag cagcttttta
    23881 tccgcccgca tacgcgcaca ccgggcatcc cgctgcgctg gatcaccgcc gccgatgtcc
    23941 tcgcccagca agccttattg cgacacccgg attttgtcgt cgcccgcatg aaaggccagt
    24001 actggcaagt gcgtgaaaaa gtgttcgatt acgaaggtcg ttttcgtcgg gcgcacgaat
    24061 tacgcggtta atggtagttt tgattgatgt ataaggcgat tgagtataga atctcgcccg
    24121 cttttgcata cgcaaagcag atcacttacc tgttgctggg tcgcctgtag caggattaat
    24181 ttaagagaga aagaaatgtt tactatcaac gcagaagtac gtaaagagca gggtaagggt
    24241 gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa tcatctacgg tggcaaagaa
    24301 gcgccgctgg ctgtcgaact ggatcacgac aaagtcatga acatgcaagt taaagctgaa
    24361 ttctacagcg aagttctgac catcgttgtt gacggtaaag aaatcaaagt taaagctcag
    24421 gacgtacagc gtcacccgta caaaccgaag ctgctgcaca tcgacttcgt tcgcgcttaa
    24481 ttgctgaata agttgtaaaa aaccccgctc cggcggggtt ttttgtctct gcaaattatg
    24541 cctgattacg gtattgctat tttttgcagg ccagataagg cgttcacgcc gcatctggca
    24601 ttcagaacac aaaaccgatt aattgccgcc agacgtccgg cgctgcaatt gatcgcgcaa
    24661 gttcggcggc gtgcctttaa tggtcagggt atcggttgct ggatcccaga agatgcgttc
    24721 acctaacagc atcgcatcaa agttaatagt caagccaccg ccgctaccgg caaacttcgt
    24781 caactgacgc agtgtgctac ggtctgccgg gaagctctct tccagttcgt agcctttttc
    24841 agccgcaaac tccgtgaagc tcacttcgct gacgcctgcc agctctttcg acagcgattc
    24901 cagttcaatc tcttcacctg cctgtagctg ctcattacag tagctataaa cctgctggcg
    24961 cacgttctgt cgctctgctt tatccagttg tgcttcggca gtgaaatcat cgaccgcttg
    25021 caacagccca cggttttgcg ctttcgcgtt taacccttcg ctggcaccga ggaaatccat
    25081 aaagaaatcc gccactttgc gccctactcg ccctttcagg aaagtgagat agcgggtcga
    25141 ctctggattg gtttcccact cggttaaatc gatacgcgca acaatatccg catgattgat
    25201 atcaagataa tgggttgggt tgatgtcgag attttcgtta acacgcatac tgctcaggtt
    25261 gctcagaact gccaccagca gatactccac cgccagatag cgatagtggc aaaacagcac
    25321 gaatccaccg tcagcaaaag gatatttcgc taattcgtca cgcaggcgac cggttgccgc
    25381 gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt aagcgcagcg tctgcgccag
    25441 ttcactctct tcgctaaaca agccgtaagc tttatttttg gcgctataga cccgatgcag
    25501 ttctgccacc atctcaacga cggtttccgt cggttccagc aatgaatcgc gcaacaccag
    25561 ctcaaggttc tgctcatcac gcttgataag ctggtgcaga gcaatctggt tgatatccag
    25621 actcatgata aactctcctt taagaccggg cggtattcaa ccaccgccgg ttaagcgacg
    25681 caataaaaga tggcggtcat tttacagaag gcggcgtaac gagaatgatt taacgcataa
    25741 aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc ctttcatcaa ctaactgatt
    25801 tcgatttatg ccacaaattt cccgctacag tgatgaacag gttgaacaat tgctcgcaga
    25861 gctgctcaac atactggaaa aacataaggc tccgaccgac ctttccctga tggtgttagg
    25921 caacatggtc actaacctta tcaacaccag cattgccccg gcccaacgcc aggcaattgc
    25981 caactctttt gcccgcgcct tacagtcctc tatcaacgaa gacaaagcgc actaagggaa
    26041 acagataaca ggttatggta actcatcgtc agcgctaccg tgaaaaagtc tcccagatgg
    26101 tcagttgggg gcactggttt gcactgttca atattctgct ttcgctcgtc attggcagcc
    26161 gttacctgtt tatcgccgac tggccgacaa cgcttgctgg tcgcatttat tcctacgtaa
    26221 gcattatcgg tcatttcagc ttcctggtgt tcgccaccta cttgctgatc ctcttcccgc
    26281 taacctttat cgtcggctcc cagaggctga tgaggttttt gtccgtcatt ctggcaacgg
    26341 cgggaatgac gctattactg atcgacagcg aagtctttac tcgtttccat ctccatctta
    26401 atcccatcgt ctggcagctg gttatcaacc cagacgaaaa tgagatggcg cgcgactggc
    26461 agctgatgtt catcagcgtg ccggttattt tattgcttga actggtgttt gcgacgtgga
    26521 gctggcaaaa gctgcgcagc ctgacgcgtc gtcggcgctt cgcgcgcccg ctggccgcat
    26581 tcttatttat cgcctttatc gcctcgcatg tggtttatat ctgggccgat gccaacttct
    26641 atcgcccgat caccatgcag cgcgctaacc tgccgctttc gtacccgatg acggcacgac
    26701 gttttcttga gaagcatggc ctgcttgatg cgcaagagta tcaacgccgt cttattgagc
    26761 aaggcaatcc agacgccgtt tccgttcagt atccgttaag cgaactgcgc tatcgcgata
    26821 tgggcaccgg gcagaatgtg ctgttgatta ctgtcgatgg cctgaactac tcacgcttcg
    26881 agaagcagat gcctgcgctg gcaggttttg ctgagcaaaa tatttcgttc acgcgtcata
    26941 tgagctccgg caacactaca gacaacggca tctttggcct gttttatggc atctcgccaa
    27001 gctatatgga cggcattttg tcgacccgta cgcctgcggc gttaattact gcgcttaatc
    27061 agcaaggcta tcagctgggg ttattctcgt cagatggctt taccagcccg ctgtatcgcc
    27121 aggcattgtt gtcagatttc tcgatgccga gtgtacgcac ccaatccgac gaacagaccg
    27181 ccacgcagtg gattaactgg ctgggccgct acgcacaaga agataaccgc tggttctcgt
    27241 gggtctcttt caatggcaca aacattgacg acagcaatca gcaggcattt gcacggaaat
    27301 atagccgggc agcaggcaat gtcgatgacc agatcaaccg cgtgctcaat gcactgcgtg
    27361 attctggcaa actggacaat acggtggtga ttatcactgc cgggcggggt attccgctga
    27421 gcgaagagga agaaaccttt gactggtccc acggtcatct gcaggtgcca ttagtgattc
    27481 actggccagg cacgccggcg cagcgtatta atgcgctgac tgatcatacc gatctgatga
    27541 cgacgctgat gcaacgcctg ctacatgtca gcacacctgc cagcgaatat tcgcaaggtc
    27601 aggatttgtt caaccctcaa cgccgccatt actgggttac cgccgcggat aacgatacgc
    27661 tggcaattac caccccgaaa aagacgctgg tgctgaacaa taacggtaaa taccgcactt
    27721 acaacttacg tggtgaaaga gtgaaagatg aaaaaccaca gttaagtttg ttattgcaag
    27781 tactgacaga cgagaagcgt tttatcgcta actgattaat tataaatcag ttagcaaaat
    27841 atcttacttg caatcggtgt ggaaaacggt agtattagca gccacgagtc ggcacgtagc
    27901 gcagcctggt agcgcaccgt catggggtgt cgggggtcgg aggttcaaat cctctcgtgc
    27961 cgaccaaaaa tcccaagaaa accaacctat tgcggttggt ttttttatat ctgcatttaa
    28021 ttcgataaac agaccgcgac acatcacggc ctgtttattt tctgttatca gaacgtccag
    28081 accacacccg cctgagttgt gctgctttct tcacgggaga aacggctgtc gataccaagc
    28141 gttagccccg tcaatgtttg ctcaaagcca gcgcctgcat cagtggtcac gttgttgcgg
    28201 ctgttaattg ccgaactcca catcgccgta tcgtaattaa cgccttttac gctaccaaga
    28261 cgcccacgca cggtgtccag ttctgcatca aataccagcg gttgtgctgc cgccatattc
    28321 agcacatccg tggttgaagg gggcatttgt gcgcgattct ctgcgagact ccagctatgg
    28381 ttgccattat ccagcaaggt atattcatac gtactgatat caacaacgcc tccggcattg
    28441 cccaacgtaa atgccgcatc accgcgcccg ttgttaccag tgtaaggtta tctcctgctg
    28501 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgct ttaacctcca
    28561 gtagccctcc cttttctata acggcaaaga cggcaatccc atctttttca acaattaagc
    28621 gccctccttt aatcgtggga tcagtcgccg cgccataaac ctgtagcact ccattattga
    28681 tcgtagtctg gtttaccgcg ccacccattt gaacaatttg ataaccatca tttaatactg
    28741 agtattctgc ggtgccattt atttcaatgt actgatatcc cccgttaatt tcagtattac
    28801 tacttacacc aaattcttat atatgctgtt ctccgccagg attgattttc gtattattgg
    28861 cggccctaca aaccagctgg atatcttttt ccaggacaac accatcaact gtctcaccat
    28921 actcaacagt tgatgccaat gctatgggcg aatataacgc tgtagccacc agaacagata
    28981 ttgcggaacg acaaagagaa acagaaccag attggtgcat taagctttca tcctatgaaa
    29041 ttaattgctg ttaaaagcat tggggacaga aaatacccat agctccatac ccggagtcag
    29101 tttttaaaaa ctgtttaaag aaatgcacaa gtattgtgat tgatttttta gttgtttttc
    29161 ctgatgagaa gctgatgcaa aattccgtct ttataataaa gatgatgcca aagcgaacga
    29221 caaagttgta gttttcacta catgtccata cataaaatgg ggtaacattc acgcgcctgg
    29281 tagcgttacc aacgctacgc tcaaacataa tgattctaat aaaacctcag gagactacta
    29341 tgcctgaagc aacgcctttt caggtgatga ttgtggatga tcatccactt atgcgacgcg
    29401 gtgttcgtca gttactggag cttgattctg gctttgaagt ggtcgccgaa gcgggcgacg
    29461 gcgcaagcgc gatcgatctg gcgaatagac tggatatcga cgtgatcttg ctggatctca
    29521 atatgaaagg catgagtggc ctggataccc tcaatgcctt gcgcagggat ggcgttaccg
    29581 cgcaaattat tatcctgacc gtatcagatg cctccagcga tgtctttgca ctgatagacg
    29641 caggcgcaga cggttatctg ttgaaagaca gcgacccgga agtgttactg gaagcgattc
    29701 gtgccggagc gaaaggcagc aaagtcttta gcgaacgcgt caatcagtac ttacgtgaac
    29761 gtgaaatgtt tggcgcggaa gaagacccct tcagcgtgct gacagaacgc gagctggatg
    29821 ttctgcacga gctggcacag gggctgtcaa ataaacagat tgcctcggtg ttgaatattt
    29881 ccgagcagac agtaaaagtc catattcgca atctgctgcg taaactcaac gtccgctcac
    29941 gcgtggcggc caccattctg ttcctgcaac aacgcggggc acaataaaaa tagcccgatg
    30001 gcatcaccat cgggccactt tttatttact ctcctgcggc gacaaatgtt gcatcgcctg
    30061 cgcgatacta cgttcgatca ccgcacggcg agtatcgttg gcaggtaaga gtttcaacat
    30121 catttcccac gcggcaacgg cttcgccaaa tcgttgctgc tcaaaggcat taaatgcata
    30181 catgcttaac acacggatat tgctatggtc cgttctcacc aactggcgca gcagttcgcc
    30241 gccgaggcgg ttgtcgttgg gatcagatga acgtgtcaac gcttcagcgt atcccagtgc
    30301 agcatcgctg tttttcggat cgaggcgata cgcagtggcg tatgcatcgg tggcgatact
    30361 ggcgttaccc agcgccatgc caacgcggcc caacataatc cagccttcta tatctcccgg
    30421 atttttttgc agttgagtac gcatccccag cgcaaggcgc gacatctctt cttcgttgag
    30481 cggatcggct ttcggatcca gcgccctgtc cagtaacgcc ggagcctgtg ccgtggcctg
    30541 ctgccagatt ttcacctgct gataattgcc agtctggtag tagctgacgc cagccacaat
    30601 taacgccacc acaatacccg gcagataaac aacatatccg gcacgcttac cttccggcac
    30661 gctttgttct ggaaacgctt ccggcaccac gcgtacccga cgccgcgaac gggcgtatat
    30721 gacccaaccg ccaatgccaa tagccactac tggcagcacc cacagcagca cggtcagcgg
    30781 cgttaacggc ggatcgtaag tgacgaagtt gccgtaacgc gccaccatat aatcgacaat
    30841 ctctttctta cttttgcctt cctgcatcag ttcatacact ttctggcgca ggtcggtggc
    30901 aatcatcgag ttggaatcgg caatgctgtt gttctggcat ttcgggcagc gcagttcttc
    30961 agtgagctga cggaactgtt gttcctgtgc ttcatcttta aactgcaaca cgtcgatggt
    31021 cgccagcgct gagccggaga tcatcagcat cagcacgccc aataaaaacc tcattgtgcg
    31081 gcctccttac tgtatttctc ccacagtggc ttgatctctt cttcccagac gcgaggattg
    31141 agatcgcccg catggcgata gcgaatgatg ccgttgccgt caataaggaa cgtttctggc
    31201 gcgccataga caccgagatc cagccctaac atgccgtcgc catcaaacag gcttagcgcg
    31261 taaggattgc ccagctcttt cagccagctg attgccttct ggcgatcgtc tttatagttc
    31321 atgccgacca cgcggatgcc ctgcgcagaa agctgattca gatattgatg ttccgcacgg
    31381 caggtcggac accaggtcgc ccagacgtta agcagcactg gtttgccctg agtcagcaca
    31441 tccgcctgat aaaactgccc cggattgtcc agtgattcga ggcgaaactt cggcactggc
    31501 ttgccaatga gcgccgattc cagattggtc ggatcatccc cttcggcatt acgcgccagc
    31561 tgccacagca gcgccgcggc aatcgccagg aagataatca acggaattaa caataccttg
    31621 cgcttcatgc agcctccggc gcagttttct gcgggttcac gcgcttacga tagcgaggat
    31681 caaacagaca cagcagtccg cccaacgcca tcatcagccc gcccgcccaa atccagcgaa
    31741 caaatggttt gtagtaaaga cgcacggccc acgcgccgtt ttccagctct tcaccgagcg
    31801 ccgcgtacag gtcacgcgtg atgccaccgt caatcgccgc ttcggtcatc atcgacccgg
    31861 cagtgttgta ataacgtttt tctgcataca gcactgtttc cggcttgcca tctcgcgtta
    31921 cgccgatagt cgccacaccg ccacgccagt ttggaccagt cacttctttg acatcacgga
    31981 aggtgaagcg atattcatga atatcgacgc tatcgccgga cttcatgcgc acatcacgct
    32041 caacgctata gttctgacta aaagcaatgc caacaattgt cactgccagc cccaggtgag
    32101 ctgccaccat cccccaataa ctgaaggtgg ttttcgtgcc gcgtgaaata cgcagcgcag
    32161 cttccgcaat tgccagcacc gcaatccagc aggccattgc caggccgagc accgtcatcg
    32221 ccacaacttt gctttcgaac agccacggca acagcagcga cagcaccagc gtagagatga
    32281 aggcgataat caacaaattg cggattttac gtgggcgatc gcgtccccag cgcaccagag
    32341 ggccgacacc aagcagtagc gcaaacggca ccatcagcca ggtaaacatg gtgttgaaga
    32401 acggttcgcc aatcgaaata ctgcccagtc ccagttgctt gtgcaccagc ggcagcaacg
    32461 tccccagcaa caccaccagc atcgcggcta ccagcaaaac gttgttcgct aacagcaaag
    32521 attcccgcga ccacagcgca ttgtttacgc gtgagcgaac tttgtgtcca cgcgcggcaa
    32581 acagcagcag cgaaccgcca atcaccagca ccataaaggc gaggataaac ataccgcgcg
    32641 ccggatcaga cgcaaacgcg tgtaccgata ccagcacgcc ggaacgcacg aggaaagtcc
    32701 ccagcagaca caacgagaag gcactgattg ccagcagtaa tgtccacgct ttgaagctgg
    32761 cgcgttgttc agtgaccgcc agtgagtgca tcagcgcagt ccccaccagc cacggcataa
    32821 acgaggcgtt ttctaccgga tcccagaacc accagccgcc ccagccgagt tcgtaatagg
    32881 cccatgcgga accgagcaca atgccgagcg tcaggaagat ccacgcagcc agcgtccacg
    32941 gacgagtaaa acgcgcataa gtgctgtcca gacgcccact caacaaagat gcaatggcaa
    33001 aagcaaacgc caccgagaaa ccgacgtacc ccatatagag cagaggcggg tggaaaatca
    33061 gccccggatc ctgcaacaac gggttaagat cgcgcccttc aatcgggaaa ttcggcagcg
    33121 tgcgtgagaa cgggttagag gtaaagagaa tgaacagcaa aaagccgaca ctgaccatcc
    33181 ccattatcgc cagtacgcgg gcaacaatat ccagcggaat acgctgacta aaaatcgcta
    33241 ccgcaaaggt ccagccgctc atcagtagca cccacagcag gagcgagcct tcatgcgcgc
    33301 cccaggtagc cgccacgcga taccacaccg gaagctgggt attggagttg ctggcaacat
    33361 aggtgacggt aaagtcgttg accacgaaag cattgaccag caccagaaat gcgccagcca
    33421 cagacataaa cagcagccag gcaaacaagc gggaagacgc catcatgcgc gcgtccccgc
    33481 gcgccacgcc ccatagcgga tacacagaca gcagcagcgc aattcctagc gccagacaca
    33541 gcagcccgtt accaatttct ggcatcatga tgctgggtcc ttataaacac tcgccgggcg
    33601 acggtggtta gcttccatcg ctttctcaac ttctggcggc gtgtagtttt catcgtgttt
    33661 cgccagcact tctttcgcga ggatatgatt gcctttttcc agttcgccct gcaccacaac
    33721 gccctgccct tcacggaaca gatccggcaa aatgccttcg taagagacat ctactgaacc
    33781 ttcagcgtcg taaatggtga aagttacttt tagcgaattg ggatcgcgct gcacactccc
    33841 cggcatcacc atcccgccaa cgcgcagacg ctgaccgact tccggcatct gctgagtttc
    33901 acgcttgccg tagagaatct cccccggcgt ataaaagaga tcgatattcg agcgcagcgc
    33961 atatagcacc agaccgatag tcagcgccag ccccgccagc acggcacagg caatccacaa
    34021 gcggttttta cggcgaatat tcatgcagcc tcctgctgtt gcgcagcacg taaacgcgcc
    34081 tcacgcgccc gctgttgcgc cacgccacgc agaattgcgc gatgttgcat caccgagtgc
    34141 acgactaaaa ccaccagcgg aataacggtc atcaccaccg ccagccagac aaaaaaggcg
    34201 taaccgccca ttgcgaaaaa ttcattccag gaagcaaatg caggggtcat ttacggcctc
    34261 ttttcagtat cagttcactc acccacggac ggcgtttttc catcagcaaa atcaaattac
    34321 gcatccgcat cagcgtcagc gtggcagaca ggagcaggaa gcccaaaatc gaccagcgca
    34381 gcggcgaacg catcgccgga tcgatacttt gctgcattcg cgttgatccc tgatgcaggg
    34441 tgttccacca ctctacggag taatgaataa tcggcagatt caccacgcca atcagcacca
    34501 ggatacctgc cgcacgccca gccagacgac ggtcgtcgaa ggcgtgccac agggcaatga
    34561 caccaacata cagaaacagc agcaccagtt cagaagtcag acgtgcatcc catacccacc
    34621 aggtgcccca catcggtttt ccccatgcag agccggtaac cagggcaata aaggtaaaca
    34681 cggcaccaat gggggccatc gccgccaccg ccaggttggc cattttcatc tgccagacaa
    34741 ggccaataaa cgccgccacc gccattgatg cataaatgcc catcgaccag atcgccgcag
    34801 gcacatgcag gtagataatg cggtagctat ttccctgctg ataatcagcc ggagcaaagc
    34861 cgaaccccca gatccagccg acggtaagca cgaccacact ggcaattgcc agccacggta
    34921 taaaccagcc acagatttga tacagccgtg gtgggatcgc cagttgatgc agtgttttcc
    34981 acatagtttc gataccagac tcgaacaaaa atcagtaatc cagcgttatt gaatgctgat
    35041 tcgtaacgct gccgccgtcg caaaaggact taatgtcgcg gtgcctgcca gcaacgcgcc
    35101 taaaattgcc agatacccat caacgggcaa atgcatagaa gccgcgtcca tcgcggcggt
    35161 ggcaaagatg agtaatggga tagtcagcgg taacaccagt atgctgagca gcacaccacc
    35221 gcgcttaagt cccactgtca gcgccacgcc cggcgcacca agaaagccaa gcgtaggggt
    35281 tcccagcagt agcgttaacg ccatcacttg ccagccataa acatccattc ccagtagcat
    35341 tgctaccagg ggcgaaagga tgagtaacgg cagaccggtt accatccagt gcgccatcac
    35401 cttcgccagc acaacggcgg gcaagggtaa cggcaacaac atcaattgtt caagactgcc
    35461 gtcctgcaaa tcgtcacgga acagtcgctc gagtgccagc aaggatgaaa gcagcgcggc
    35521 aacccagata atacccggcg caatacgcgc cagcagttgc ggctccggac cgatactgag
    35581 cggaaaaagg gtaatgacaa tcaggaagaa ccacagcggg ttggcgattt cggcgctgtg
    35641 gcgaaacgct acgcgcagct caagacggaa aatgcgccag aacatcatgc ggccctcgtt
    35701 tgcgtcagtg aaatgcggcg aattttactt tcagcaacgt tgagcggctg gtgggtagtc
    35761 agaatcacaa tccccccctg ctccgtatgc tgcgccatac gctgggtcag acgatcgacg
    35821 ccattaacgt caatcgcggt aaaaggctcg tcgaggatcc ataacgtggc acgggtcagc
    35881 cacagacgcg ctaaagcgac gcggcgttgt tgcccggcag aaagttgatt taccggaata
    35941 tcttcgaatc cggcaagccc ggcctgcgcc agggcttcca gacattgtgc ggtatcgcca
    36001 tcgcgatgat aaaagtgcag attttctaac gccgtcagcc gggttttgat ccccggctga
    36061 tggcctatcc ataacaggtt ttgatggtag ctgtcgcgta cctgatgcaa gggctgccct
    36121 tgccagagaa cctcgcctgc atcagggcga gacaaccccg tcagcaaacg gagaagcgtt
    36181 gtcttccccg cgccgttgct accggtgatt tgtacccact ctcctgcgtt cagcgtaaat
    36241 gataaaccac taaataaggt tcgttcatcc cgctcgcaaa gtaactctct ggcttcaagc
    36301 atactcacgc aataaccctg ttaaaaacct ggctcgactt cacgcatgtc gggcagcttg
    36361 tgcgctatcc ctttatggca atcaatacag gtttgcccat ctttcaccgc ctggtcatgc
    36421 atcttcgcgg caaccgattt ctgggcggtt gtatccatat actcgaagtt gtgacagtta
    36481 cggcactcct gcgagttgtt gtccttcatg cgccgccact cattctgtgc catcgtcagg
    36541 cgatgagctt caaatttctg cggcgtgtca ataacgccaa aaattttacc atacagctct
    36601 ttacttgctt tgagcttgcg tatcatcttt ggcacaaact cgtgcggaac gtgacaatcc
    36661 ggacaggtcg cacggacgcc gctacggttg ttgtagtgca cggaatccat gtattcctga
    36721 tacaccgtgt tacgcatttc gtggcagcta atgcagaact cttcggtatt ggctttttcc
    36781 atcccggtgt taaagccgcc ccagaagacg atgccaccga caaaaccgat caacagcagc
    36841 gtccccagcg ccagacggct gggggtacgc caccatttcc acaggcgctt aatcagacca
    36901 ggcttacggt cagaatttcc cataataacc tcttatttcc cgtaaccttt tgacggggta
    36961 aaggtattcc ccacgattgg cgcggtatcg gcctgcggta cgtgacattg cagacagaaa
    37021 taacgacgcg gagccacttc cgcgcccact ttgccgtcgc tgtccataaa gtgagtagga
    37081 ctaatgcgcg gcgcgccagt ggtgcgataa ctttcgacac cgtggcactg caagcagcgg
    37141 ttggtattgg tcgttacctg ataaccttca acgctatgcg ggatcattgg cggctgattc
    37201 acatagttca gcggcatccg atcctgctct tttggcatcc gaatggcccc ttcctgcgtc
    37261 ccggatactt ccggcgactg gctaaaatcg acgccattag ccgcccaaac cgcgccgctt
    37321 accaccaggg ccagcatcgc cgtccattga cacagcgctt tcttcaggtc atggcttttc
    37381 atgatttcgc tcccgaactc catcgtgtag ttattgtaaa tacatcctca gaacagacat
    37441 ccacgcagcg accgcacgtc atgcaatcgc ggctggtgac ctgcaccggg ctttgttcat
    37501 ccagcaccgg ggcacgtagc acatgcggtt ccgggcaaac atgaaaacaa tccatacagc
    37561 ggttacattt ctgccgatcg gtggccgcaa cggtaatcac acctttgcta cccagcacgc
    37621 catacagtgc accaaccgga caaatgtgcc cgcaccagcc gtgttcaacg accagtagat
    37681 caaataaaaa cagcgcgaga ataagcagcg cgccgctgcc gaagcccatt accaggctac
    37741 gccccatcag ggaaacgggg ttgatccatt cccagatgag cgtgccagtt aaggccgatc
    37801 ccaccagaat gatcaccaac agcacgtagc gaatgtggcg agggatcgtc gcagactgat
    37861 tgaggtcaaa tcgtctgcgt aaccaatttg ctaagtcggt aatcgggttc agcgggcaga
    37921 cccagctgca aaataatcgc tttcctgcca gggcgtagag cacggtgata atcaccgcac
    37981 cagtcagcgc taccgtggca ggcagatgac cgctggcaag actttgcagt gtcatcagcg
    38041 gatcggtcag tggaacggtg tcgaataaca ggctactgct gtagttgccg tgcaagatcc
    38101 acacaccaaa ccacggaccg ctcaaaaaca tccccagcac gaagaactga caaaggcgac
    38161 gtaacactaa ccaacggtga ctgcgccacc agcctttttt ctccagcgcc tcgcgcccgg
    38221 cgtcacgttt acgatttgcc attgttcccc tccagccagc cgaagcggta atggtgacct
    38281 aactcccctt tcgccagtga cagcggtaac accttgattg ccggttgttc cagcacgcac
    38341 actttttcgc acttaccgca gccggtacag gcgtcgctgt gaaccgtcgg cagaaagcgg
    38401 gcgtgcttac cggttcgcgt gttgcgctcc agctccaggg tgatggcctc atcaattttc
    38461 gggcattcgc gataacaaac atcgcagcgc agcccctgaa agttgagaca gttttcctgg
    38521 tccaccagta ccgccagccc catccgcgcg tcgtcgatcg attcgatctc acgatccagc
    38581 gcaccgctcg ggcacacttt ggcgcacgga atgtcctcac acatttcgca aggaatatcc
    38641 cgtgcgacaa aatatggcgt gcccgccgac agaccagagg ccagcgtcgc cagttttaag
    38701 gtgtcgtaag ggcaagcctg aacacactga ccgcaacgca cacaggcact ggcaaaggcg
    38761 ttctcgttta tggctccggg cgggcgcaac cgcacaccag atgcgcgtgc ggtttgctgt
    38821 tgtaacccca gcgccacacc cacggcagcc agcccgcctg ctgtgcgaac gacatcgcgc
    38881 agaaagcggc ggcgaccatt ttgaggtttc gctgaccggg acataatggc ttacaccttc
    38941 tccagtttga ccgcgcactt cttgaagtcc gtctctttcg agagcggatc ggtcgcatcc
    39001 agcgtcagtt tgttaaccag ctgtgcggcg tcgaagaacg gcatgtacac caggccctgc
    39061 ggcggacggt tacgaccgcg cgtttcgaca atcgagatca cttcgccacg gcgagaaacc
    39121 actttcactt tgtcgccacg gcgcagatcg cgcgctttcg catccagcgg gtgaataaac
    39181 agaaccgctt ccgggaaggc gcggtgcagt tccggtacac ggcgagtcat actgccggta
    39241 tgccagtgct ccaggacgcg tccggtagag agccacaggt cgtactcttc atccggcgct
    39301 tccgccgcag gttcgaacgg cagcgcgaag atcaccgctt tgccatccgg tttaccgtag
    39361 aacttgtagc cttcgcccgc tttcacgtac gggtcgttac cttcgctgta acgccactgc
    39421 gtttctttac cgttcaccac cggccagcgc agaccgcgtg ctttgtggta atcatcgaac
    39481 ggcgcgagat cgtgaccgtg accgcgacca aaccatgcgt actcttcgaa caaccctttt
    39541 tgcagataga agcccagctc gcgagattcg tcgttcagct gatcttccgc cagttcggat
    39601 accgggaatt tgctcacttc cggagtggca tacagaacct catacagcgt tttgccacgc
    39661 agttccggtt tcttagccag cagctcttcc ggccacactt cttcagtttt gaagcgacgg
    39721 gagaactgaa ctaactgcca gagatccgat tttgcttcac ccggtgcctg tacctgctga
    39781 cgccagaact gagtacggcg ttcggcatta ccgtaagccc cctctttctc tacccacatt
    39841 gcggtcggca ggatcaagtc cgctgccagc gcactgaccg tcggatatgg atcggaaacg
    39901 atgataaagt tgcgcggatc gcgccagccc ggcatacgct cttcattaat gttcggtccg
    39961 gcctgcatgt tattggtaca catggtccag taaacattca gtttgccgtc tttcagcgca
    40021 cggtcttgtg ccaccgcgtg cagaccgatt ttcgccggaa tggtgccact cgggatattc
    40081 cacttcttct cgcaaatatc acgatgtttc tcgttagtca ccaccatgtc cgcaggcaga
    40141 cggtgagcaa aggtgcccac ttcacgcgca gtaccacacg cggaaggctg cccggtcagg
    40201 gagaacggac cgcaacccgg ctgggaaatt ttgccggtca gcaggtgcag gttgtagacc
    40261 aggttgttgg cccacacacc acgggtatgc tggttgaagc ccatcgtcca gtaggagatg
    40321 actttcttgt tcggatcggc atacagctgc gccagttgtt ctaactggtc tttcggcacg
    40381 ccggtcattt cggcggtttt ttccagcgta tattcagcaa cgaaggcttt gtaatcttca
    40441 aagctcatcg gttcggaggc gtcagaaccc ggattcttcg ctgctttttc cagcggatgg
    40501 gtcggacgta aaccgtagcc gatgtccgtc gcccctttgc gcaggttaac gtgtttgctg
    40561 aagaagtcct gatttatcgc attgttttga atgatatagt tggcgatata gttcaggatc
    40621 accaggtcag attgcggcgt aaagatgatg ccgttatccg ccagctcgaa gctacgatgc
    40681 tggtaggtag aaagcaccgc cacggtaacg ttctggttag agagacgacg gttagtgatg
    40741 cgcgaccaga ggatcgggtg catctccgcc atgtttgcgc cccacagcac aaacgcgtca
    40801 gcctgctcga tgtcgtcata gcagcccatc ggctcatcca taccaaaggt acgcataaag
    40861 ccaactactg ccgacgccat acagtgacgc gcgttcgggt cgatgttgtt cgaacggaag
    40921 cccgctttaa acagcttgga cgcggcataa ccttcccaga tagtccactg accagaaccg
    40981 aacataccga tcgattccgg ccctttttct ttcagggcgg ttttgaattt ctcttccatc
    41041 acatcgaagg cctgatccca ggtgattggg gtaaattcgc cttctttatc atatttaccg
    41101 tttttcatac gcagcagcgg ctgcgtcaaa cggtctttac cgtacatgat tttgggcagg
    41161 aaatagccct taatgcagtt caggccacgg ttaaccggtg cgtccgggtc gccctgacaa
    41221 gccaccacac gcccctgctg cgttccgacc agaacgccgc aacccgtacc gcagaaacgg
    41281 cacggcgctt tatcccattt aatggcttcc tgctgaccaa caacggcgcg ggcaacgccc
    41341 ggcacgctga gaccggcagc cgccgcagcg gccgcaacgg cgttagcttt cataaagcta
    41401 cgacgactga gtttcatggt gtttcctcac cttgctcttc ctgctggtga taaaccagcg
    41461 acaccgccag cacgccctct acgttgcgta ctgactcaat ggtttggatc agcgtttcgc
    41521 tgtcttctgc ttccaccacc acaatcaact gaccgctcgg cgcgtcgctg acagcaactt
    41581 cacagccggt aaaggcgttc agttgggtgc tgatgtctga aattcgttcg cttttggcct
    41641 gcacgaccag gctgcaaact tgccagttag tgtgcatgga gatactccgc agttatggct
    41701 gataccggac agctggctgc acatgctcca catccgttgc aaagttggct attaagttgc
    41761 ggctggtaga tcccggagag cgtcgggcga aagataattg ccattggctc acagctatcc
    41821 tgacagcggc ggcattcaac tgactgatac gccaggcacg cgtccccaat ggtgaactgc
    41881 aaatcccagg ccctggtgtg gcgcggagaa aatagcgatt cggggcaagc ctgcgcgcag
    41941 gcgtaacaga agctgcactc gttatttttg aaattaacgc tcggatagct acccgcgccg
    42001 cgttgcagaa tgttgttttc acaagcatta atacaggcgt cgcagcgggt gcaatgggtc
    42061 agaaaatgag attcatcacc ggaccagggc ggacggatac cgttactggc tttgcgccag
    42121 cgaccagtga gtatgccccg acgggatgca tcaatcttca cattgacctt ccatcattaa
    42181 cgcgctctga aaattgagag cgaccaaata aaccgcataa ttaataagcc atttttatag
    42241 ccgctaagat attaaaggat gtgtcaaaga tgcatacccc gatcggggta aaacctgtag
    42301 caggatcaaa agagtggcgg gaagcgtggc aaaaacgggc ttttgctcac atttcaaatg
    42361 gttataaaca tatttatata gcgattaatt cgccagagat atttctgctg gtttgttttc
    42421 tcatcagaat ttaacactaa aagagcaggt aaaattatct gaatgttctt taagttattc
    42481 ataaagcaaa ttaataaatc tgatgaatat gttaaccttc agcgacatca tcggtgaaaa
    42541 cctataaatg aagaaggaaa gcaaaaaaat gaagaccatt ctacctgcag tattgtttgc
    42601 cgctttcgcc accacttccg cctgggcggc agagagcgtc cagccactgg aaaaaatcgc
    42661 gccttatcca caagctgaaa aaggcatgaa gcgtcaggtg attcagttaa ctccgcaaga
    42721 agatgaatct accctgaaag tagaactgtt aatcggtcag acgctggaag tcgactgcaa
    42781 tttgcatcgt ctcggcggga agctggaaag caaaacgctg gaaggctggg gctatgatta
    42841 ttatgtcttt gataaagtta gctcgccggt ttccacgatg atggcctgcc cggatggcaa
    42901 gaaagagaag aaatttgtca ccgcgtatct gggcgatgct ggaatgctgc gttacaacag
    42961 caagctgccg atcgtggtgt atacgccaga caatgtggat gtgaagtacc gtgtctggaa
    43021 ggcggaagag aaaattgaca acgcggttgt tcgctaaact gctttgaagt gctgcaacct
    43081 gtaggccgaa taaggcggtt acgccgcatc cgacatctaa cgcccgagcc ggttgcctga
    43141 tgcgacgctg aagcgtctta tcagcatacg ccacatccgg caaaccatgc cggatgtggc
    43201 acatcattac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt tcaactgcgg
    43261 tttcggcgta ctatctgctg cctgcggctt gtcgtatttc agacccagca cttcgctggt
    43321 gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc cataagacgg
    43381 aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc caaatacttt
    43441 ttccagcaga ttcaacataa tcggcgcggc ggttgacgcc cctggcgatg cccccaggag
    43501 cgcggcaatg gtcccttgct ggtcactgac gacttcagta ccgagacgca gtacgccacc
    43561 tttatcggca tcacgcttga taatctgcac gcgctgcccc gcttgccaca aacgccagtc
    43621 ctcttttttc gcctgcggat agtactcttt caacacttca aaacgatcct cttcactcaa
    43681 catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccaa cgtgcatcat
    43741 cggcatcacg ttagaggtgg tggtggaact catcagatcc cataatgaac cgtttttgag
    43801 gaatttggtt gagaaggtgg caaatggccc aaacagcact acgcgtttac cgtcaagaac
    43861 acgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt taccgtaaac
    43921 cttcgccaga tggtgattaa ccacgtccgg gttttccgaa acaaggaact gtccgcccac
    43981 cgggaaacca gcgtagtctt tcgcctccgg aatccccgat tcctgtaaca gtttcagcgc
    44041 cgcaccgccc gcgccgataa agacaaattt ggcgcggatg ttctgtgcag tgccgttttt
    44101 cagatcggca acggtgacgg tccagctgtt atcgtcatta cgctttaggg cgcggacttc
    44161 gctgctgagt tgcagcgaga agttagattt cttctgtaag gaggcgatca actggcgggt
    44221 gatctcgcca tagttcacat cggtgccaat ttctgtgcgt gttgccgcta ctttctgttg
    44281 cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt gatcttcaga
    44341 gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg cgcgcaggaa
    44401 attaacgtta tcctcgcccc agacaaagct catatgcgga acggtattga taaatgaacg
    44461 cggagtacgc agcacaccac gctcgacctg gtgcgcccag aactggcggg aaatctgaaa
    44521 tgcttcgtta atggcgactg ctttttcaat actgatactg ccgtcggcat tttgcggggt
    44581 gtaattgagt tccatcagtg cagaatgccc ggttccggcg ttgttccagc cgttcgaact
    44641 ctcctgcgcg acaccttcca ggcgctccac catggtcatc gaccattcag gctccagctc
    44701 gcgtaaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca gtacatcagt
    44761 ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa gccccacggc
    44821 catcgagaag agcatggcag tcactttttt catcttgtta atgccttact tttagtcgct
    44881 ttattgcagg tgagatttgc gcggcatcaa cggtaacact taagtaacaa catttaaata
    44941 atgtttataa attatattct aattttagaa acattataat ttcgttgatt aattataggg
    45001 ttattagaaa cgaaggtatt tt
//
LOCUS       DAOFSV010000029        41593 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.029, whole genome shotgun sequence.
ACCESSION   DAOFSV010000029 DAOFSV010000000
VERSION     DAOFSV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.029"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1764
                     /locus_tag="QBH27_001648"
     CDS             <1..1764
                     /locus_tag="QBH27_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HDQ7013970.1"
                     /translation="LTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLY
                     QNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTA
                     RREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLSG
                     ILDQLTLMADARQSPLIALTDTIAWQVATGRENRGLSDSLAKSAQELFNGKEKTPQQS
                     REGDVPAGPLDKTFAPLLRLLGDKSGGGGGDTQLNLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAAN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLERAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPG
                     QVFLTGRSMKDAEEYGEDADE"
     gene            1757..3367
                     /gene="tssA"
                     /locus_tag="QBH27_001649"
     CDS             1757..3367
                     /gene="tssA"
                     /locus_tag="QBH27_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDQ7013971.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLSVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPVRAAVLCDTQHKD"
     gene            3373..4791
                     /locus_tag="QBH27_001650"
     CDS             3373..4791
                     /locus_tag="QBH27_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HDQ7013972.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMINGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSERQTGKYFTKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLKTSANEEDWVDIEVNKNNKTVNIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVDLTKNGVEFYWSRNGSRGGGIGESIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            4784..5206
                     /locus_tag="QBH27_001651"
     CDS             4784..5206
                     /locus_tag="QBH27_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7013973.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDSQNICP"
     gene            5766..7526
                     /gene="tssF"
                     /locus_tag="QBH27_001652"
     CDS             5766..7526
                     /gene="tssF"
                     /locus_tag="QBH27_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDQ7013974.1"
                     /translation="MDDLTQRYFEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLSADSPVACALHRALTLGTQQFWLRLPGQGRRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     LLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLAAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            7490..8569
                     /gene="tssG"
                     /locus_tag="QBH27_001653"
     CDS             7490..8569
                     /gene="tssG"
                     /locus_tag="QBH27_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDQ7013975.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQRQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVDNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            8550..9086
                     /gene="tssJ"
                     /locus_tag="QBH27_001654"
     CDS             8550..9086
                     /gene="tssJ"
                     /locus_tag="QBH27_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDQ7013976.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            9090..9518
                     /gene="tssE"
                     /locus_tag="QBH27_001655"
     CDS             9090..9518
                     /gene="tssE"
                     /locus_tag="QBH27_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDQ7013977.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVRERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            9518..10894
                     /locus_tag="QBH27_001656"
     CDS             9518..10894
                     /locus_tag="QBH27_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDQ7013978.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVSSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNVQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(11194..12141)
                     /locus_tag="QBH27_001657"
     CDS             complement(11194..12141)
                     /locus_tag="QBH27_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDQ7013979.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKILA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHAIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGRKIKNVL"
     gene            complement(12213..12809)
                     /locus_tag="QBH27_001658"
     CDS             complement(12213..12809)
                     /locus_tag="QBH27_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDQ7013980.1"
                     /translation="MNNTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            complement(12812..13987)
                     /locus_tag="QBH27_001659"
     CDS             complement(12812..13987)
                     /locus_tag="QBH27_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDQ7013981.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGSYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IGYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLTRLVSVIKNINQGE"
     gene            complement(13987..15567)
                     /locus_tag="QBH27_001660"
     CDS             complement(13987..15567)
                     /locus_tag="QBH27_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDQ7013982.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKIAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
     gene            complement(15599..16423)
                     /locus_tag="QBH27_001661"
     CDS             complement(15599..16423)
                     /locus_tag="QBH27_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDQ7013983.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEE
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLSIRTKVLMD"
     gene            complement(16681..17934)
                     /gene="amiC"
                     /locus_tag="QBH27_001662"
     CDS             complement(16681..17934)
                     /gene="amiC"
                     /locus_tag="QBH27_001662"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDQ7013984.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            18166..19497
                     /gene="argA"
                     /locus_tag="QBH27_001663"
     CDS             18166..19497
                     /gene="argA"
                     /locus_tag="QBH27_001663"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDQ7013985.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQTRVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(19579..21405)
                     /gene="recD"
                     /locus_tag="QBH27_001664"
     CDS             complement(19579..21405)
                     /gene="recD"
                     /locus_tag="QBH27_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDQ7013986.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTAEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEVLAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(21405..24947)
                     /gene="recB"
                     /locus_tag="QBH27_001665"
     CDS             complement(21405..24947)
                     /gene="recB"
                     /locus_tag="QBH27_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDQ7013987.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGEMQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDVAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEVPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(24940..27828)
                     /gene="ptrA"
                     /locus_tag="QBH27_001666"
     CDS             complement(24940..27828)
                     /gene="ptrA"
                     /locus_tag="QBH27_001666"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDQ7013988.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQAITLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKIADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(28004..31372)
                     /gene="recC"
                     /locus_tag="QBH27_001667"
     CDS             complement(28004..31372)
                     /gene="recC"
                     /locus_tag="QBH27_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDQ7013989.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(31385..31708)
                     /locus_tag="QBH27_001668"
     CDS             complement(31385..31708)
                     /locus_tag="QBH27_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDQ7013990.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(31693..32100)
                     /locus_tag="QBH27_001669"
     CDS             complement(31693..32100)
                     /locus_tag="QBH27_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDQ7013991.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(32097..32660)
                     /locus_tag="QBH27_001670"
     CDS             complement(32097..32660)
                     /locus_tag="QBH27_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDQ7013992.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(32651..33121)
                     /locus_tag="QBH27_001671"
     CDS             complement(32651..33121)
                     /locus_tag="QBH27_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDQ7013993.1"
                     /translation="MKKQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(33306..34100)
                     /gene="thyA"
                     /locus_tag="QBH27_001672"
     CDS             complement(33306..34100)
                     /gene="thyA"
                     /locus_tag="QBH27_001672"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDQ7013994.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(34107..34982)
                     /gene="lgt"
                     /locus_tag="QBH27_001673"
     CDS             complement(34107..34982)
                     /gene="lgt"
                     /locus_tag="QBH27_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDQ7013995.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(35133..37379)
                     /gene="ptsP"
                     /locus_tag="QBH27_001674"
     CDS             complement(35133..37379)
                     /gene="ptsP"
                     /locus_tag="QBH27_001674"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ7013996.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(37392..37922)
                     /gene="rppH"
                     /locus_tag="QBH27_001675"
     CDS             complement(37392..37922)
                     /gene="rppH"
                     /locus_tag="QBH27_001675"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDQ7013997.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            38607..39296
                     /gene="mutH"
                     /locus_tag="QBH27_001676"
     CDS             38607..39296
                     /gene="mutH"
                     /locus_tag="QBH27_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDQ7013998.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            39365..40078
                     /locus_tag="QBH27_001677"
     CDS             39365..40078
                     /locus_tag="QBH27_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ7013999.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            40216..40434
                     /gene="ygdR"
                     /locus_tag="QBH27_001678"
     CDS             40216..40434
                     /gene="ygdR"
                     /locus_tag="QBH27_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDQ7014000.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            40542..41582
                     /locus_tag="QBH27_001679"
     CDS             40542..41582
                     /locus_tag="QBH27_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDQ7014001.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHSLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
BASE COUNT        10453 a        10850 c        10330 g         9960 t
ORIGIN      
        1 ctgaccttct ggatggcgaa cctgccggaa cacccgcagt ggaaaacctc accgccaccg
       61 gaactgaccg gcgcggtgcg taaaatcctg ctgcgccaga ttggtgtgcg taatgccgaa
      121 aacaccctct accagaacgt gctgcaacag gtgtcccgca actacgccga tatgacgctg
      181 gcggacatga ccggggatac cctcactgaa tctcttttca gtacggaaca gacggtgccg
      241 gggatgttca cccgtcaggc atgggaagga caggtcaggg aagctatcga gcaggtggtg
      301 acggcgcggc gcgaggaaat cgactgggta ctcagcgacc gccagcagga tacctctgct
      361 gatatctcgc cggatacgct gcgtaaccgc ctcacctcac gctactttac cgactttgcc
      421 ggaagctggc tggcgtttct caacagcatt cgctggaaaa aggaagactc gctctccggc
      481 attctcgacc agctgacgct gatggccgat gcccgtcagt cgccactgat tgcactgaca
      541 gacactattg cgtggcaggt ggcaacaggc agggaaaatc gtggcctgtc agactcgctg
      601 gcgaaatcgg cacaggaact gtttaacggc aaggagaaaa cgccgcagca atcccgtgaa
      661 ggcgacgtgc ctgccgggcc gctggataaa accttcgcgc cgctgctgcg gttgctgggc
      721 gataagtcag gaggcggtgg tggtgatacg caactcaacc tgcaaacgta tctgacccgc
      781 gtcacccgcg tacgcctcaa actgcaacag gtgaccaacg cccccgaccc gcaggagatg
      841 acccagcaac tggcgcagac ggttttacag ggcaaaaccg ttgacctcac cgacacccgc
      901 gactacgggc ggttaatcgc cgccagtctg ggcgaagaat ggagtggctt cggtcaggcg
      961 ctgttcgtcc gcccggtgga acagtcgtgg cggcaggtgc tgacgcccgc agcggacagc
     1021 ctgaaccgcc agtggcaacg ggcgattgtc agtcactgga atcaggactt cgctggccgc
     1081 tatccgttca aagcctcaca gaacgatgcc tctcttccct tgctggcgca gtacctgcgc
     1141 gatgacggac gcatcaacca gtttatcgcc gccaaccttt ccggtgttct gaaacgtgaa
     1201 ggccgctact gggtggctga cgccatgaac acgcaggggc tgacggtcaa cccggacttt
     1261 atccgcgccc tgaaccgcct gcgcgatgtg gccgataccg cctttgccag cggcgatgcc
     1321 ggaatacatt tcgaactgcg ggcaaaaccg gcgcgtgacg tgatgaaaac gcatctggtg
     1381 attgacgggc aggaactgga gtatttcaac cagaaagaac gctggcagcg ttttaactgg
     1441 ccggatgaac agtggcaacc cggcgcatcg ctgagctgga ccagcacaca ggcgatggaa
     1501 cgcatactgg cggattaccg gggaagctgg agccttattc gcctgctgga acgggcgcag
     1561 gtaacaccgg tcgacagcag cacctttaaa gtggtgtgga aagcgcagga cggcctgcca
     1621 ctgaattacc tgctgcgggt tgaacagggt aaagggccgc tggcgctgct ggaactgaaa
     1681 aacttccgcc tgccgggaca ggtatttctg accggcagga gtatgaagga tgcggaagag
     1741 tatggggaag acgccgatga gtgaactgtt gcaaaagctg acccgtagct gttttgccga
     1801 ccgggatgca ctggatgtgg cgcgaacaca ggcggcgctg tggcagacat ggctgctgcc
     1861 ggtgacggca gacacgccgg tcggggaaga ccccggctac cacgacgatt tcctgcgtat
     1921 ccgcgacgaa atgaacaaac tgtccggtgc cgataccggc cttatctgcc agcttgccga
     1981 atccctgctg ctgacgcagg ccaaagacgt gcgcatcgcc acttactaca tctgggcgcg
     2041 actgcaccgc gacggtgagc gcggactggc tgaaggactg gcgctgttag ccgggctggt
     2101 ggaacgcttc ggtacgcaac tgctgccctc gcgaccggca agccgaaaaa tggcgctgga
     2161 gtggctggcg ggggaaaaga tgctcgacag ccttgcccgc tacccggagg tggcaaaaga
     2221 ggattttgcg aacattgttg ccgctcttaa tcaactgtcg gtttccttca ctgcatggcc
     2281 cgaagaccag cattccccct ccctgatgcc gctgattaac gcgctggaat cacgtctggc
     2341 gcagtcgggc gggatgaacg cggtggtgcc gcaaaacagc agcggcgttc cggcaccgtc
     2401 ttccccggtg gatgcgccgc aggtacagac catcacctcc gggcgcgacc tactcgacca
     2461 ggcgaaagtg ctggcccgtt atctgaacga acagccgcag ggctggcttt cagcacaccg
     2521 gctgatgaaa accctgcgct gggacacggt gcatgaactg ccgccggacg tggacggcaa
     2581 aacccggctg gccccgccgc gcaccgaatc acgcaaccag cttaagcggc tgtatgccca
     2641 gcagaactgg acggaactgc tggagcaggc cgacctgatg ttcagcaccg gtgtcagcca
     2701 tttctggctg gatatccagt ggtatctgca tcaggcgctg gcgaaggcgg gcgcaccgtg
     2761 ggacagatgg acagcggtta tccgtcagga cctggcgctg ctgctggagc gcctgccggg
     2821 gctggaaaat ctggcatgga acgacggcac gccgtttgcc gatgaggtga cgcgcaactg
     2881 gatagcgcag caggtcatga tgcgggaaga cggcgcatgg cttgcgggga aagccgctgt
     2941 gccgacagac gacgccacaa atgacgtact ggcgctggag ccggaagcac tggagatggc
     3001 tgacagtcag ggcgtggaag cggcgctggg ctggatacag acccgtccgg gcatcaccac
     3061 ggcacgacag cgcctgttat tgcgactgct gatggcacgg gtggcggaac agtacggtaa
     3121 aaacgagatg gcgctcctgc tgctggaaga actggatacc gccgcacagg gaataacgct
     3181 gacacagtgg gagccagagc tgctgtttga agtaaaagcc cgacaactga aactgctgcg
     3241 tctgcgggcg caccgctatg cggataaagc cctgctgaac cgaaaaatgg aaatcctgct
     3301 gggaacgctg gtggcaatcg atccggtacg ggcagcggta ttgtgtgata cgcaacataa
     3361 ggactgatgg caatgggaac aggctacttt ctggtcaggg gggataagac cacctgtggt
     3421 ggaaaaatta ttgaaggggc ggacgaccat acgatcatgg gtatcccgca ggcacgggat
     3481 atggaccggg tcacctgcgg cagatatccg ggaatgttta ttattgtggg cggcgttcct
     3541 gaaacagata ttcacggcag gctgatggcg gggacgctgg acagtcagag cagttgcccg
     3601 tgtaaagcac ggtttattgc gtcaatgatg gatgatacgt atgagacgga tgacggggaa
     3661 agtgaagcgg aacaacatgc gcagtcggcg aaaaaggatc tgacttctgg ttctgattca
     3721 tcctctgatg atgtaaaact ggattacaga attaaattat caggtaataa gatactgacg
     3781 ccactgaata tacctgatta taaagaaatg ataaacggtg gtagcacgaa gaatacagaa
     3841 aaaatagatt ttaccatcac aaataaaggc gatgaggctg aaggactttc cctggaagtg
     3901 cttgatggca atgaagtgat ttatagtgaa cggcagaccg gtaaatattt tactaaggga
     3961 gagcatgcct ggcaatggga tggctacagt aatcaaggca tactggatac aactaagcta
     4021 aaaagtaaat cactactcgt aagactgatt gctttatgtg gtgatatgat gataaaagtt
     4081 gattatccat tgaaaacttc tgcgaatgag gaagattggg tagatataga agttaacaaa
     4141 aacaataaga cagtaaatat aatatggcga ctggcagtta gtgatggagg gataaaagga
     4201 agtaatccta agttatcccc agttccttat aacgacttgg ttgacctgac caaaaatgga
     4261 gttgaatttt attggtctcg aaatggaagt cgcggcgggg ggattggtga aagtattgtt
     4321 acagcgattg gtgtttttaa agtaaatgta aaggctgaga taaatattac tccatccatg
     4381 cgaacttttt ctttgatatc atcattagat cctgactttc aggcatccgt tagtctatca
     4441 ggatttgaaa aaatatatta taactacggt gatagttata aggatattca agatgaatta
     4501 caggcacttc ttgacgctaa taacagatat aaatgggata gtgctcatga aatggggcat
     4561 aaagtactgg atgaatatgg agagggttct agccctgatt attcgtggac tcataaagga
     4621 acatctactc ttatgcaaaa aacaatacca gggaatgtta tgcccgctca gggagagatt
     4681 gatgtaatga aatatggaaa atatcgtcct gacatgtata ctcgattggt tgcagcagat
     4741 gaagatgttc aggggttgat ctggttaagc aggataaaat ttgatgacta gagtagttat
     4801 tgctttatta tttcttgccg gatgtacttc gcaaatgaag tattccaatg tcgtcaggat
     4861 taggaatgta tgttcctcag atattaatgt gtttattcct gagtatgttt ttggaggtaa
     4921 tgttattaag caacatcata tttacttacc tgttaatggg attgatgttt tgtcttattt
     4981 ttatactgaa aacatcaatg atggtttcca atatgaagaa gcattcaccg taaataaaga
     5041 tagctcgggt aattttgact atccgtttgt tatcttatca tcaggacgtg aaaaaaaatt
     5101 tgggaaaaac gatctaattg aattatccag ggacatttca acaaaagacg ataagaagca
     5161 tggggttgta aagtacatga tagatagtca gaatatttgc ccatagtatg attcatatca
     5221 taatcaactg aaaaattgac attattaatc atttgtaatt ttttgatact accaagtgaa
     5281 atttgatgat tagattaata ttctcttttt tattatgcac actgtgtgct ggatgtttat
     5341 ctcaaccatt ctattcttgt tccattgtca tggtaaatac atgcaaagta tctattagca
     5401 tttcgacagt taataattca acacattatg atggtaaagt aagagataat ttccatcttg
     5461 atgtcggaga acgaaagcca attgtatact ttatttacac aggtagagag tcagatgttt
     5521 ttaataaaaa ccctgtgaat attgtgtctg aacttagtat tttgatatct gatggtaata
     5581 attctaaaac tttatctgga tatcatatat cctctttatt gaagaaatcc tcatctagta
     5641 tggagtccga tttttctttt gaaataaatg atccatctat atgtccttag gattctgaaa
     5701 ataaaaaatt ctaatctcac tactcgttgg tattaatttc aataaaagga aagataaatc
     5761 ctccgatgga cgacctgacc caacgctact ttgaggccga aatgcgctac ctgcgtgaag
     5821 cggggaaaga atttgcgcag gcttaccccg accgcgctgc gatgcttaat cttgataaac
     5881 ccggcgcacg cgatccctat gtggagcgcc tgttcgaggg cttcgctttt ttaatgggcc
     5941 gcctgcgcga aaagctggac gacgatctgc cggaactgac tgaagggctg gtcagcctgc
     6001 tgtggccgca ctacctgcgc actattccgt cgctttccgt ggtcgaactg accacggatc
     6061 accaacaaat gaagcaaagt gatacgctga aagagtttca ggtgctatcc cgtcccatcg
     6121 gcgaacgcag aacccgctgc gtctacagcg ccacgcggga tatcacgctg catccgctgg
     6181 cgttgccgga cgtgtcattg caatatgaac cggacggacg ctcggttatc cgtctgcgct
     6241 ttgaatgcgg cccgctggtt ggtgactggt cacagattga tttatcccgc ctgccgctgt
     6301 acctcagtgc cgacagcccg gtggcctgtg cgctgcaccg ggctttaacg cttggtacgc
     6361 agcagttctg gctgcgtctg ccagggcagg gacgccgtgt gctggacgcg cacttttccc
     6421 cgatgggttt tgatgacgat gaccgactgt ggccgaaagg cgaatcggcg ttcagcggct
     6481 accagttgct gctggagtat ttcaccttcc gcgagaagtt tatgttcgtg gcgctgaacg
     6541 ggctggagca ggttgcatgg ccggagggga taaccggctt tgagattgac gtgttgctga
     6601 acgagaactg gcctcacgac ctgccgtttg acagcgataa tatccgtctg cactgcgtgc
     6661 cggtcatcaa cctgtttccg ctggaagccg atccgttaca cctttcgcca ctggaaaacg
     6721 aatttctgct gcgcccgatg cgcattcagg acgggcatac cgagatttat tccgtcgaca
     6781 acattatttc ttcgcgccat accggcagtc aggcgtatgt gccattctcc agctttcgtc
     6841 accggggcgg gatgctgcgc cacgatgcgc cggaacgcta ttaccacacg cgggtaaagc
     6901 gcggcccttc cggcctgcat gacacctggc tgattctggg cggcgatgcg tttgatactg
     6961 accggatgct ggaggatgaa accctgtcct taagcctgac cggtactaac gggcagcttc
     7021 cccgtaaagc tttacagagc acgctgctcg atacgccagt tcatgcctca cagaacgtcc
     7081 tgcgggtgcg caacctgtgc gccccgacgc agccctgcta tcccccggcg cgtgaccgct
     7141 tccactggcg ggtgctcagc caccttggtt ctaacttttt atcgatgatg gataacgccg
     7201 aaattctgcg cggcacgctg gcgctgtacg actggaccga aagcgagatg aaccgccgtc
     7261 gtctggcggc gattgtcgac gttcagcaca gcctgataca gcgttttgaa agaggctttc
     7321 tgctgcgtgg cgtggatatt caggtgacgc tggacagtaa cggctttgcc ggagaaggtg
     7381 atatcacgct gtttggtgaa ctgctgcacc gcttcttcgc actgtatgcc gatatccact
     7441 tattcactca actaacctta attctgcaac ccacaggaaa gtgcctgcaa tggacagagc
     7501 atcacagcca gcgcgtaccg ggctgaccga ccggctggcc ccggatattg cgcggatcaa
     7561 tttttaccgt ttttgccagt tgctggaaca gcgccagcag aacgcgccgc tgggcagtac
     7621 cgacagcccc gccgccgatg cggtgcgctt tcgtcctcat ccggggatgg gctttccggt
     7681 cagcgagtta aagaacgttg aacgtgacgt ggacaatccc gacgcgccac ccacagtgcg
     7741 caccactttt ctcggcctgt acggcgtgga ttcgccactg ccaaccgcct atctggacta
     7801 cattacccag cgtcatgacg gacatgacgc ggtgatggcg tttctcgata tttttaacca
     7861 tcgctttatc acccagtatt accgcatctg gcgcaaatat aactacccgg cgtcgtttga
     7921 agcaggtgca gtggatgaca tttcccgttg cctgctggga ctcatcgggc tgggtattcc
     7981 cggcagcgag aaccatatcg ccacgccggt atcccgtttt ctggccctgc tcagcgtgat
     8041 gcgcctgccg acgcgcacgg cggaaggcgt tacggcactg gtgggactgc ttgccccgct
     8101 gacgaaagcg acggtggtgc ctcatgatcc gcagcctgta attctcccgg cccctgcggg
     8161 tctgtcaaaa aattcaccca tcagcctgaa aacgcgaacg ctgctggggc gtaccggcac
     8221 cgacgtcaac agccagttgc tgctgaaact gtataccgaa gatgccgcag aagcacgtgg
     8281 ctggctgccc ggcgggcaac tgcacagcga tttgctggtg ctgctgcgcg tttatctggg
     8341 ctggcgctgt caggcccgac tgcaactgac gctgcccgtc agcctgttgc ctgctgcccg
     8401 gctgggaaaa cagcgtgtac agattagccg taccggcatc cttcgcgcct cctttgccgc
     8461 gcccgcgacc ggaacagtga cggtcagtct ggggcgttat cagggattaa tacccgcttt
     8521 ttccattcgt aacagggaga gtatgactca tgtttcctat tcgttttaaa cgtccggcgc
     8581 tgctctgtat ggcgatgctg acggttgttc tgagcggctg cgggctgatt cagaaagtgg
     8641 tggatgaatc gaaaagcgtg gcctcagccg ttttctacaa gcaaatcaaa atactgcatc
     8701 tcgatttctt ctcccgaagt gccctgaata cggatgcgga agatacgccg ctttccacga
     8761 tggtgcatgt ctggcaactg aaaacccgcg aagattttga caaggcggat tatgacaccc
     8821 tgtttatgca ggaagagaag acgctggaga agaacgtact ggcaaaacac accgtctggg
     8881 taaaaccgga aggcacggca tccctgaatg tgccgctgga taaagagacg cagtttgtcg
     8941 ccattattgg gcagttttat caccctgatg aaaaaagcga cagctggcgt ctggtgatca
     9001 aaagggacga actggaggcc gacaagccgc gctcgattga actgatgaga agcgacctgc
     9061 gactgctgcc tctcaaggat aaataaccga tgtcgacacc ttccttatat gaaatgctga
     9121 cgttcagttt cagcggcgag ttaccgctgg aacaggtcag agagcgggat cagcttatcc
     9181 tttccgtgat ggacaatatg caacgcatta tcaactgccg ggcaggcacg ctggcgcatt
     9241 tgccggatta cggtctgccg gatctcagtc tgatccatca ggggatggct gcgggtattc
     9301 atggcctgat gcgtcagatt gaagaaacgc tgctgcgtta tgaaccacgc ctgagtcaga
     9361 tacaggtgga gttactcccc cagccccgtc cggggcatct taattacctg atccacgcgc
     9421 agcttcccga taccggctgg atacgctttg atggcgtatt ttctccggaa ggacgaattg
     9481 ttctgcgtca tctcaaacaa caggagcggg cgtactgatg gcaagtaacg cgaattttat
     9541 cagccagttc gtcatgggcg gcgatccctg tacttataag gaatccggtg aactgcaggc
     9601 tgaaatgagt aaactgactc acccggcccg accggatgtg gactggcgtc aggtggaaaa
     9661 actctgcctc gcgctgttcc ggcaaaatgg cgtggaacta cagacgctgg tctgttacgt
     9721 actggcgata accagacggc aggggctggc agggatggca gacggactcg gctcactgga
     9781 tatactgctc cagcgctggg ctgacttctg gccggtacag gtacattcca gaatatcact
     9841 gctcagctgg gtcacagaaa aaatgcagca ggcactgaga acgctggata ttcagtatca
     9901 ggatctgccg cagatctacc gttgtgtaca gcatctttcc gccatcgaaa ccacgctgca
     9961 acagtgtgaa ctgtggcata tgacgaaact ggatgtgctg gccgggcagt ttcgcaatac
    10021 cgcattgcgt ctggaacggc tggcgcctca gggagcggaa accactatca ctccccctga
    10081 attaccccgc cgggaaatga atcaaccgaa aaagtcagaa gaaagtccac agccggtttt
    10141 tgcaacgaga cccgctcagc aaaacgataa ggatgccagt ccacccgtgt catcccctga
    10201 aatctcccga cagcggacat ggccgatatt tatggccgga atggttgtga tggccggtct
    10261 cggcggaaca ggattatggg gctggtcgca gcttaatcag ccggacgcgc taatccagcg
    10321 aatacaactg tctgtcatgc cgttgccgca gtcgctggag agcggcgaac tggcaaagct
    10381 ggatgtaaag gataaggcgc tgctggctca ggacagaaca attgcggcaa gtcagatgca
    10441 actggagcag ttaaacaaat tacctgcccg ctggccgctg gagcagggat atcgccagct
    10501 acgccagctt gatgccctgt ggccggataa tcctcaggtc agggcgctga acgtgcagtg
    10561 gcgtaaacag cgggaactga gcgccctgtc cgcagaggca ctgaatggct acgcacaggc
    10621 gcagagtcag ctacagcgcc tgtcggcaca actggatgca ctggatgaac gtaaggggag
    10681 atatctgacc ggttcggaac taaaaacggc ggtgtacggc atccggcagt cgttaaagga
    10741 gccgccgctg gaagaactgc ttcggcaact ggaagagcaa aaacagaccg gagagatttc
    10801 gccgacgctg ttgacgcaga ttgatacccg gttaaatcag ttgctgaatc gctatgtcat
    10861 tttactggat acgaaggtgg aacaaagtca gtaaacatcg ggggatttat gtaccaataa
    10921 gggatatgtg accttgctat tttttaaaag gagtctttgt gtacaaaaaa gagaggaaca
    10981 ggccattcga tctactcgtt gattcacatc agataatgag tgtatatttc ggtagatagc
    11041 ttgggttcgg gaacagtgaa taccttaagt ttcaaaagat tggattaacc tcttgactgt
    11101 ggcccccgca accaatttat tagaatgctg acaaaccgca atgtgttcta caaattgcgg
    11161 tttttttgta tttaaaatct ttgttcgctg aactcacaac acgtttttta tcttccttcc
    11221 tgcgaagtaa tcaattatcg actgggcaac gtgtatcccg gtttgctggt atgactcaac
    11281 ggtggcggcc gcaatatgcg gcgttgtaat gatatttttg gcatgtagca gtgggctaaa
    11341 ttgcgccaac ggttcagagc taaacacatc cagcgccgca aacacatttc gttcttcaat
    11401 cacctgaagt aaagcgccct catccagcaa ctttcccctt gcggcattga tgatcagagc
    11461 accattgcgc atcatcgaca aatatttatg actaatgaag ttctccagct cagaagaaaa
    11521 aggcaaaaga acaatgatgg catgcgactg cataaagata tcttcaattg atacaaactg
    11581 aactccagct ttgtcagcaa cctgatagtc gggttgtttg tcaaaagcta aaagctcaac
    11641 atcaaaaccg gataaccttt tcgccagaat tttacctata ttaccgtagc caaccaggcc
    11701 aatccgtttc ccttgtaatt cacagccatg cgactcaccc cagtagccat tttgcatcgc
    11761 atgatagctg ccagggatat ttctcatgct cgcgaagata agtccaatga taaattccgc
    11821 aaccgcattt gaattaatcc caaccgcatt attgacaaca ataccggatt gctgtgccgc
    11881 acgaagatca atattgtcta tccccgtccc gaatctcgat attattttca caccagacag
    11941 caatgatagc gtgtccgcct gataacaatc ttttccggcg atgaccgcgc aaatagattc
    12001 tcgtaatgca gtggattctg atttaagcag atgctccaca ttattcttaa taataagatt
    12061 aaaattattc tctcttagca acttgctggc ggaaacacag cgtgctgaaa agctggggac
    12121 ggtgaccaga acatttttca tagcggacac tcacttcaaa gaacgccacc ccaaaagatg
    12181 gcgtgaccgg ttactgtatt tatattttga aattatgaat ttgctttaat gccgagtgca
    12241 cctcctggat gatataagcc gaaatcagca cgagtaaatt ttttcaattc agagagtgca
    12301 agcgccaccg catcaccaac aaccagaaca acagtcgacg agcatgacgg tgctaaacct
    12361 aaattatccg cctcactttt gacggggatt tcaacagata aatcacaact tatcgcaagc
    12421 gatgagtggt gactgcgggt aaaagagatc aaataattac ccattttttt cagactaggc
    12481 agtgtcgcca ggatttccgc cgtctcacca ctgtttgaaa taagaataac aacatcgtct
    12541 ttcgccacca ttccaaggtc gccgtgtaca gcttccgtac cgtgaacaaa aaacgaggga
    12601 gttccggtgc tggcaaatgt tgcggcgagt ttcctggcaa taataccgga cttaccaaca
    12661 ccaataaaaa caacttttcc ctgacaagat tgtaatactt taattaaatt tgcgacctcc
    12721 gaaagagggg aatcaataac ttcttcaact gctttatgtt cgttacgcat aaaatgttta
    12781 atgagatgga taagatccgt gttattcatt tttattctcc ctgattaata tttttaatta
    12841 cactcacaag acgcgtaagc cccatatata atttttctcg actgcaggcc aggttcagac
    12901 gaaggtagcc ctccccctcc gttccataga catgccccgc cataatgccc actttgcctt
    12961 catgaattaa tcgactgtag aattcatcaa aaggataacc aatagcagaa caatctatcc
    13021 aggaaaaata gcaaccatca gctggcctta cgatcaattc tggaatattt tcattcagat
    13081 actgctgggt gaattgaatg ttagaggcaa tatgtgcctt taatgacttt aaccatccct
    13141 cacattcgtt atagccaata atagttgcaa ttatattcag tactgatggg gagtttacaa
    13201 aatcgcgata cctcgatatc gttctgaact tatgattaaa attatcgtca gttgaaatca
    13261 tatacgatcc tgttagtgca ggaacattaa atgttttcga tatcgagtta aatactacta
    13321 ctttttcttt ttttatttta agcgcggatg taaagctacg attaaaaaca aaatcactat
    13381 ggatttcatc tgaacataac cagacattat gccttgtaca gcttgcagcg attttatgca
    13441 gttcttcttc cgaccagact gttcctgtcg ggttatgagg attacataat aacaccatct
    13501 ttgcgctttt aatcttaaga tcaaaatctt gccagttgat agcgtagctc ccctcatttt
    13561 ttataatcaa agaggctgtc agaatatttc gctcgctatg tttaattaca tcaaaaaatg
    13621 cattatagac aggtgtaaaa acgagaatat tatcaccatg cgatgtcaat aactctattg
    13681 cttttgcaat agagtacatc acactggggc tataggttat ccattcaggt ttaatatctg
    13741 tttgatattg ttgataatac cagtttacta tcgcattcag atattcacca tgttgccaac
    13801 gactgtaccc taaaatcggg tgttccaggc gtttttttaa tgctctggtg atagcgtctg
    13861 gtatcggaag atccatatct gaaatagaga atggtagtac atcttttaca ccaaatctgt
    13921 cctcgcaaaa gtcccattgg gtacaatatg ttcctttgcg ggaaacagga gtattaaaaa
    13981 catccatcag tatatcccca tatagttatc catatcccga cgcagggcga tgactttagt
    14041 gccgataatg acttgtaggg tattttgatc aagtttaaca accgcaatcc cgccagctat
    14101 ttttattgca acttcgtcaa tcaatcccat atctgcgatt gttaaacgta aacgagttac
    14161 gcaattatcc agtgacttaa tattctcttt accaccaagt gccttaacca tctgctccgc
    14221 cgcttgcagc tctgatttat caggagttgg gatatccgct atagccgtat cttcgtgagc
    14281 gttcagcgtg acaggactgg actcttcacg gcctacggtc atcaggttaa atttaagaat
    14341 ggtccaacgg aacacaaagt aataaatgaa gaaataggcc acttcgacac acaccgcgat
    14401 cggccaacct gttcttgtgc cacgcaatac gccaaagcta ataaattgaa tcagatcgcc
    14461 agtaaatccc atcttcacgc ccatatatgg cagaaccatg ttagcgaatc cattcataaa
    14521 ggcatggaag acaaacagca gtggactgac aaataagaac agaaactcaa tcggttcgct
    14581 gacgccgcca ataatcaccg taagcacccc ggaaataagc aatgctttga tagcctttct
    14641 gttttgagca tgagcagtgc gatacatcgc cagcgcggca ccagccagac catactgaat
    14701 catcagtttg ccctgctcca ttttccccgc caaatccagc ggaatgaccg cattctcttt
    14761 gacgtacgcc ataaacatgt tcagggtgcc gtaatattct tcgccgccaa tcacagccga
    14821 accgccgatt ggcgtaaagc ggaaaactga cgtcaccaga tggtttaagc caaaaggaat
    14881 cgtcacgcgt tcggcaactg catagaagaa ataaccaaca ggaccggaag ttgaaatcca
    14941 gtgtccaatg agcatgaaaa gattgaaaaa cggcggccag ataaaaggaa tgatcagacc
    15001 aaatccactc atcacaatca gtgtaatgat aggcaccaga cgtgggccgc tataaaaacc
    15061 taacgattca ggaatgcgta aattaacgat ctttttatac atgctggcga ctaataaccc
    15121 agcaacaatt ccccccaaca cgctggtatt gtaggactgg atccccagaa tgatggtttg
    15181 cccatgtgtc gacatttggt cagcaacgac caataagtcg tgctgtttaa gataaaagtt
    15241 cgttcccaga tgcatcgcca taaaaccaat taagccagaa aaagcaccat aggctttatc
    15301 ctctttatct tttaataatc ctaagggaat cgctatcgca aacaatacag gtaaattaac
    15361 aaaggcaaac aaaccaagac taacaatgaa atcaagtatg gttttaatta ttgggatagc
    15421 cagaaatgga attaactttg ccatatcatc actggctaaa ccacttccca gccctagcat
    15481 catgccacat acacttagca gagcaatggg atacataaat gccttcccca ggctctgaaa
    15541 aaaactccag gctttctttt gtttcatgtg ggttatctca tataaatgtt atatataatt
    15601 agtccattaa tactttggta cgaatagaga gatatagttt ttcttctaaa attaattcat
    15661 atttaaaagt ggcatacaga taccgttcaa tttcatgaat tgcgcgctgt aacaggatgt
    15721 ccttttcaag aaactgttca tagatatcac caatatcttt ctgttttatt tcttcattgc
    15781 ataacacgcg acttgaaaaa tatttaaggt gcatcataaa tctggaatag ttaaatgaac
    15841 ttctgtccac ctttctgtta taaacaactt caataagccc cgtgattatt tgcgacaact
    15901 caataattcg tactgattca taatcaactg aattttcata attatttaaa atatgcaatg
    15961 ctataaaacc aatctcatca tcaggaagtt gaacgctgaa ttgctcatta atgatagtta
    16021 atgcatcgcg acttatcgca tactctgctt tgtacaacgc tttgatttca tcaaggaaaa
    16081 cattattaat ttgtatacct gaagcgactc gggatattgc attcgaaatg tgatccgcaa
    16141 gaatcaatag tagcgatgga ttgagatttt tttcgagatt tttctccgca tattgcacta
    16201 ttttttcggc aacaaacaca tactcaatat ctacatgttc aatcaattta tagagtttgt
    16261 ttttttgttc atttctgaca taaaaaatcc ggttagccgg atgctcaggg accgacattc
    16321 catatttttt gttgtaaccg acgccaggcc cagaaatgat aacctcctgt ccattcatcg
    16381 atgcctgtac acaattattg ttcatgactt tttcgatgat cattccatgg ctccaaaaaa
    16441 aaaggcaagg cacgtcagaa gtaataacta cttcaaacat tgccttgcct gatttaacag
    16501 ttacaagatg attaactaat gcgtatacat tacacggaat tgtgcttaat taaagagatg
    16561 gtcggcttat ttttcaagcc gacattcaaa tatccgacat cagtcacaaa accagcgtct
    16621 aatattcagg taaatgttat ctattcgacg aaatataaat tcattgaata aataaaaaca
    16681 ttatcccctt ctcgccagcg tcgccccatc ggcaaaatac gctttaattc ccgcaagaat
    16741 agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt
    16801 actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta gtacggcaaa
    16861 cccagcctgt tcaacttgat ttttatgcag cttgttgatt ttaccgagct tattcagcac
    16921 cgctttacca aacttaaggc tgtcggcaat ggtcagcgac tgcaccatat cgaacatggt
    16981 gtggtcgaca tagcggtcac cgcttttgct tacgccacca atcaagtccg aggcgttctg
    17041 ggtttgtgcc agatattttg ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac
    17101 cgaggaaccg ctcggctggc gactggtaaa ggcgtcggca tggatagaaa caaacaagtc
    17161 agcgcgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt
    17221 gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca gacggcgagc
    17281 tatttgcaat accacgtctt tttcgcgtgt tttgtatttc cccaccgcac cggagtcttc
    17341 gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcccgccctg ctttacccgg
    17401 ttgtggacca ctttgtgctg gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc
    17461 cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga ggtccatcac
    17521 cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt ttacgttttg
    17581 ctttaattca aaaaccatac gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt
    17641 gatgaacggg tcgtcagcgc ggatttgcgc agccatcccc ttgagcactg agttcaggtt
    17701 tacatcttcg atatccacca ccacgcgttc aggattactc aacgcgaact gcttatattt
    17761 cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac
    17821 cgccacgacc tggctgaccg cagccaggct gacctgactt acgctcaata gccacatggc
    17881 acccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg acatgcctct
    17941 cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ccaatttgac cggaaacttt
    18001 agcgaaagac gcataatctg tcatctataa aacggtaaac attcttttta tattcacggt
    18061 attactgata aaaaactcgc tcccgcataa aatttacact tgcaccctgc gaaaaaacag
    18121 aataaaaata caataattta gaataatcat gcaaagaggt gtaccgtggt aaaggaacgt
    18181 aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac ccaccgggga
    18241 aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt ctccagtatc
    18301 gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt ctatggcgca
    18361 cgtccgcaga tcgacgcaaa tctggctgca catcaccacg aaccgctgta tcacaagaat
    18421 atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg aacattgcaa
    18481 ctggatatta ctgctcgcct gtcgatgagt ctcaataaca caccgctgca gggcgcgcat
    18541 atcaacgtcg tcagtggtaa ttttattatt gcccagccgc tgggcgtcga tgacggcgtg
    18601 gattactgtc atagcgggcg tatccggcgg attgatgaag acgcgatcca tcgtcaactg
    18661 gacagcggtg caatagtgct aatggggccg gtcgctgttt cggtcactgg cgagagcttt
    18721 aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc tgaaaaaatg
    18781 attggttttt gctcttccca gggcgtcact aatgacgacg gtgatattgt ctccgaactt
    18841 ttccctaacg aagcgcaaac gcgggtagaa gcccaggaag agaaaggcga ttacaactcc
    18901 ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt
    18961 catttaatca gttatcagga agatggcgcg ctattgcaag agttgttctc acgcgacggt
    19021 atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat
    19081 attggcggta ttctggagtt gattcgcccg ctggagcaac aaggtattct ggtacgccgt
    19141 tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagcg cgataacacg
    19201 actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga aatggcctgt
    19261 gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct ggaacgcatt
    19321 gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac gcgcagtatt
    19381 cactggttcc aggaacgtgg atttacccca gtggatattg atttactacc cgagagcaaa
    19441 aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt gggttaagcg
    19501 atgcaaatcg ccggatgcga catgcacaac attcataggt atgataagac ccacatgcgt
    19561 cgcatcaggc aattacgttt atccccgtga actaaacaac gccgccagac cactgcgccg
    19621 ctcggtgcgc gtggcaattg ccgcacttaa aatgcgctca tcggcataca gcgacagacg
    19681 gcgacgcgcg cgggtcaccg cggtgtaaac cagctctcgc gttactaccg gcgtgcgttg
    19741 actcggtaaa atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat
    19801 cgcccacgtc gtttcgtgct ctggcaggcg actcggttgc acagacttaa tattgccgtc
    19861 cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac caatatcgcc
    19921 attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc actggtcgac cttcgtacca
    19981 acgagagtgc ggatgacgat gaattttgcg cttctgttgc ataaattgct caattcgctc
    20041 attcaatcca gccacgccaa acggtccttc ccgcagggcg cacaaaagct ggtactcatt
    20101 gaacgcctga atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc
    20161 gtaacccgca agaacttcct caagcatcgc aatataatct tcgccactct gtaaaagccg
    20221 tttttcgata tcagtaaaat cctgctgaaa aacggttttc actgccgttt tatcacctcg
    20281 gttaatcgcc gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg
    20341 cagcaggcag aggctgtcgc gcaaagatgc cgcttctgtg ccagttcctg ctggaacgtg
    20401 gcttcccgtc aggcggctca actgcccggc acgctcggcg gtaaagcccg cgttggcata
    20461 agcgcagata tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc
    20521 gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc agtctcgaca tcataggaag
    20581 atcgatcatt gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg
    20641 atgacgtaaa cgctggctac ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc
    20701 ttccggaatg cgtttctttt gttcagcggt cagcggtaac tgtcgcaaag ccttgccgag
    20761 agattcggtt aagcgcgcgg cagctttacc cgttggtgca gccagacgga tacggcagcg
    20821 ttctccgtcg gccatttgaa ttaaggctgc cagcaacttc gctacggtgg tcgttttacc
    20881 ggtgccagga ccgccggaaa tcaccgaaat ccgccgcgtc agcgccactg ccgccgcaac
    20941 tttttgccag ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag
    21001 agcttcatca acctcaatgg catgattcac ttcgttgaaa aaacgtgcca ctgtgcgctc
    21061 gttacaccac atgcgattca aataaagacg atcgccacag aggatcatcg gcgttggttc
    21121 atctccccgg ctgaccgctt gtgaagccag caagcattct tcccaatttt gtagctcacc
    21181 gatttcactg acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga
    21241 aagcggcaaa caaacgtgtc cctctccggc atcatgactt aatagtgccg ccgcgagggt
    21301 gacggcagga tgttcatctc ccgccacggt cagggcaaac tgcacgtcca gcgggcgtag
    21361 ctgtttgtgc tccacagctt ccagtaattg cttttgcaat ttcattacgc ctcctccagg
    21421 gtcataccgg caaacatctc atccatcagg tcaatcaacc cggcgttggg tcgggtcgcg
    21481 tagatccctt gttgcggatg ttctttatca acgccacgca ggaacagata aataacgccg
    21541 ccaaagtggc gctcatagtc gtaatcagca atacgatggc gcagataacg gtgcagcgcc
    21601 agggtataaa gctgatattg cagatcatag cggtgtgcct gcattgccgc tgccatagcc
    21661 tgttgggtgt aagccgaact gtcttcaccc aaccagttgg atttatagtc gagcaggtaa
    21721 taacgccctt cgtggcggaa caccagatcg ataaagcctt ttaacatgcc acgtacctgc
    21781 atgaactcca gcggcgggca gccagcagat agcgggtcaa actggcgaat tagtgcatca
    21841 agctggctgg cgatgagcgg ttcgctaatt ggcaggtaaa actccatctc cacctgttta
    21901 tcgcgatcgg aaagctgact caggctaata cccgtttcat tgagaggtgc caggaggaca
    21961 gccgtgatcc actcagtcaa caccggttcc cactgcggtt caaagccacc gagctccagt
    22021 ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg
    22081 tgcaagaatg tccccggtga cgcaccgcgc gggaactgat gcggtgttaa cgtcggtact
    22141 tcaacgacgc tgaccacgcc tgcggcatca acatccaacc gtggcatcag atcctgagcg
    22201 ataccgtgac cacgctgctg caaaccggag tagctggtga cacgccagtt atcgccgggc
    22261 aatcgttgta gcgtcctcgc attcagttct gcagttaatg catcattaac ctgccagggc
    22321 tggttatcac caatttgtgc cgtttgccag gcaatatcat catcgcacaa cgcttcaatg
    22381 caggtacgaa gccctgccac atcttgcggt tctccttttt gcagcaaacg cccgagcgca
    22441 ctttggtgga cgtcggtatc acctttttta tcgccacgac ggcgcaccag cggtgcaacg
    22501 ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt aaagtaaacg cagatcttcc
    22561 gccagacgtt cggcctccgc gaggtcgacg ctttctggcg cagcattaag atccagaacg
    22621 gcctcaaacg agtggcgatc gtgataaaac gcctgatcct ggacgcggaa attggtaata
    22681 aatggcaacc agaccagcgg atattccagc ccttttgatt tgtgtatcgt gacaatctgc
    22741 accagatgtt tatcactttc gaggcgcatt tgttggctgg aggcattact gtctggctcg
    22801 aggatatgtt gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct
    22861 tcttgtagca gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt
    22921 gccagcaagt tttctgcaat gttacgcgcc gacatcagcg cccgcagcat cggcataacg
    22981 ccacgtttgc gccagatttg ccgataacca tcgaactctt cgactaccgc atcccacgca
    23041 tgttcgtcat tattcagcgt ttcaatatcc agcgcattca gccccatcat tgacgttgcc
    23101 agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa cagccaaagc
    23161 atctcctgtg cttccagagt ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg
    23221 atttccagca gcgttaaggc atcgcgcacc tgagcggcct cctggcggct gcgcaccagc
    23281 acactgatgt ccgaagcacg caccggacgc gcatcgtcgc cgttcatcag caacgcttcg
    23341 ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag cacatacctg cgccatggta
    23401 ctttgatagt cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc
    23461 tgcatttcac ctttaaacac aaagcgtaac gcctgatttt tcccggcaga tttcacagga
    23521 ataaacggta tttcacgaaa catgaacgcg tcatcagtct ggctgaaaag tttgttaacg
    23581 ctgttcacca ttcccggtgc ggaacgccag ttggtatcta aggtgtagtg agcatgaact
    23641 tcgctacgcg ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgt
    23701 ttcgggtcgc caatcagcaa caatgcggtt tccggctgat ggtgccagat acggcgaaaa
    23761 attcggtact gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc
    23821 gtacggatcg ccgctgccaa tacctcgccg ctttcactac gcagcgcgga atcgagccga
    23881 cttaacatgt catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtttcg
    23941 cggatctcag ccaatgcgcg ggtgatcacc agatcgcgga tcgacaatgg ttctgcaagc
    24001 agttgatcga tcgcctcaaa cagtggatgt cgcggggttt cccccccggc tttcgtgcga
    24061 tcttctaaga aacgctgaga gaatttttcc agcgactccg gcaactgata gctattggtc
    24121 tcttcttctg cccaggcgct gatcttctcg atccatttag cctgattgct acggttaaac
    24181 ttgcgtcgat caataccaga agattcgatc agcgcatcca gttcacccac tgcgtcgcgc
    24241 cactgctgtt ttacggcatc aatacgcgcc acaatttgcg cgtggcggga agccagcgtt
    24301 tcatcatcgg ggggcggtgc tttgataact ggcgcttcgc cttgcagata acgattaata
    24361 tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga cgacctgggc aatttcacgc
    24421 ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac
    24481 tcatcttcaa tcagctgctg ctcaaacagc atgccggatt caaaggcatt caggttgagc
    24541 atgcgctggc aaaagccgtg gatagtaaag actgccgctt catccatctg ccgctcggct
    24601 aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct cttccagcag gcgtttgtac
    24661 agtggattgt cggtggtttc acgcagacag gcgatgcgca actcgtggat attgctacgg
    24721 atacgaccac gcaattctgc cgtggcagcc tcggtaaagg tcaccaccag cagttcttca
    24781 acggtcagcg ggcggggaaa ggcggccgaa ccgcctagtc caagtaacag gcgcaaatag
    24841 agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc
    24901 tgtaagggca agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat
    24961 caggggcatt gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg
    25021 cacatattcg gctttcccat tctggctgcc ggaaatctgc gacagaatag ccataccttg
    25081 cggctcgacc accgcctgat ggaagaaatc agcaattttt tgcggcgtca gcagttttat
    25141 ctgggccacg attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact
    25201 taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg taattaccgc
    25261 ctgctggatt tgcgcaaact cttctggctt catcgcccgc aattttgcct ctgcggttgg
    25321 gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag
    25381 gaagcccatg ccccactgac gccccacgct cattggaaac gcaaacacgg catagcccaa
    25441 ctgttcttcg gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga
    25501 actatacgct gagctggtgt attcatcgta gccagtcggt acaaataccg ctgccagtgc
    25561 ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat tgttttttat cgaccacgac
    25621 atctttgtta cgacaccact ctgaaccatc agcgcccaac tgtttttgca catggcgtgc
    25681 cagcgttatt gcctgggctt cagtcatgtt gccgataacc ataaactctg gtctagcccc
    25741 tgattttaag gcgtcgcgat aggccagcac ctctttcaac gtaatggagg gcaaaatttt
    25801 gcgccgttca tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg gcataatcgc
    25861 ctgctcgaac gctttgccct tttctgcgga atccatcatc tggttatacc aggacttcgc
    25921 ctgctcaagc tgatcttccg tagcggtata gctaaagtag ccctccagca gagcctggaa
    25981 cagctgcggc agacgctggg tgtaaccatt agcattaacc ataaggccgt tgttagcatt
    26041 ggtggaaaaa cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc
    26101 tgcgagataa tcattgagtg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg
    26161 attacgcaaa atcaggctga catcagcttt gggctcgctg gcaaaataac ggcttggcgc
    26221 atacaccacg cgcagattcg actcatcaac aatcaactct ggatggtcgt atttcttctc
    26281 tgacttaatc agcgagaaat cgtcaggaat ataggggtta agctccggta aggagagcgc
    26341 aatattggcg gcttttttct gccagtcagc gaaagtttgt gcgctaattt tatcgacctg
    26401 atacggcgca tcgacaaagt aagccgtttt gttgtgcggc tctttcgggc tgatatacca
    26461 gatacgcgca ttctgtggcg tcatcatcgc cagacgttct tttaccgctt tagcgtcgta
    26521 ccgatcggca atattgactg catccagtgt atgctcaaca ggaacgcgaa tcatggtatc
    26581 tgccagccat tcgacgtaat ccatatcacg ggtgattgac ggataacgga agtcgatatc
    26641 cagcacattc gccagttcat cgaagtattg tttatcgatc cctttttcac gtaacagatt
    26701 gaggtagcta aaaatggccg ccacaacctg atcgcgattc gccagacctt tatcggttaa
    26761 agacgcagag atcgctaata cgccgctgtt gccgttgacg ataggatcgg aattggcgct
    26821 aatgccctca actaatccct gcttttgcag ccagtcagaa agtgtaccag ggctgcgatt
    26881 accaatcaga taggtaatca attcatccgt tttactacgg aacttcgctg aattgttatc
    26941 gatgcgaaac tcaacgcgca gaactttacg cggcagcgcc gggacgtaat gaatgataat
    27001 gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt
    27061 cggcacgcga ccaaaggtgt ccgccgccat tttcgccaac tccggcaacg gtttattgct
    27121 gtaaataacc gccttcatca gattggcgga atagtacttc tcgtggaaat ctttcagcgc
    27181 ctgctgtacc ggattacctg gtttgtcgct taaagtttcg aggttaccac cagaaaactt
    27241 tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc
    27301 acgcgtacgc gccatggtta attcggcatt cactgcatta cgctcacgtt cggcgtattt
    27361 cttgtcgagc aagggttctg caatagcatc ggccaggcgg tctaccgcac cgggtaaggc
    27421 atcgttctca acttccagat agaaagccgt gcgatacggt gccgtgctgg cattgtgact
    27481 gccgccgtgc attttgagat attcggccag actgtcagcc tgcggatact ttttcgaccc
    27541 catcagactc atatgttcaa ggtaatgtgc cagcccctgg tacgcctcag ggtcttccag
    27601 cgacccaacg ggcaccacca gcgccgagag cgatttaact gcctgcggat cagaaaccag
    27661 taagaccacc ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact
    27721 tttacggatg gtttcctgaa tcggctgcca tcccgtttct gcctgactta agggtgccca
    27781 gagggcaaca aacaacaata atgctttgaa ccagatgctg cggggcattc acggacctca
    27841 taatcaactt tattttctgg ccagattcaa cacgttaacg caaatcatct gccagaattt
    27901 aatcttgtgc tgaacgagtc agcctatgtc tatataacca tcagtccgtg actggtgcgc
    27961 atcataaagt aagcggatag attgcgcaat ttttatacaa cactcatgac tgattaaagc
    28021 gaaacagcgg taacaggaaa cgttgcgact gttcaacgat agcctccatt gtctctggtg
    28081 ttaattgccg ccagagccgt tgataccaga tatcatcacc ttcgccacgc accatcatgt
    28141 tgccttcgta agcctgaagg aatttcgtac gggctttttg caacgtggaa tcgtcatcca
    28201 gcatggcatc gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta
    28261 acaccagcaa tggcgcggac attccttcac gatacccctc aatcagttgt gagaggtaat
    28321 gcaaagcctg ttcggctgca agcggcggaa aacgccactc gccgtctttg cgtagaaaaa
    28381 ggcgactttc accattacca ccgctggcac agtagacaag gtgttccagc caaagttgca
    28441 ttccctgcgc cacacttaat aaagagggac gccagcgcaa caggccatcc ggctgcacct
    28501 gcggcaacca gcccgttatt tgcacaccgt tgcaggcgag atcaatctcc atactctgcc
    28561 ccggctggcg acaggcaatg actctgtcgg caagctgctg catctcctga cactgcgttt
    28621 cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcgacgga
    28681 acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat
    28741 aacggctaag tccttccaga ataaatggct cggtgtcggg gatttcgctg tcttcggtac
    28801 ggaagttcac ctgcaaacgc atctggaaaa atgcccgcac cggatgtgcc cagaatcgtt
    28861 gtagcgtttc cagcggcacg gtttccggta aggtaaatgg cagcggctga acaaattcag
    28921 aatgtgcttt accagcctgg ctggccgcag gtagccattc acgagcataa ctttgtcgtt
    28981 cgcctggctg gtagttttgt ggatcaaacg gcattcgggt atggaggcaa gtaagatgcg
    29041 cttttaccct tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt agataatggc
    29101 tttgcccgat gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat
    29161 cctgaatgga acgcccgata tagctgatat agagtttttg ctgcgcggaa attaacgctt
    29221 ccaggaacag atagcggtcg tcatcgcgac ggctacggtc gccacgcttc ggtttctggc
    29281 tcatcagatc aaagcccaat ggcgcaagct gacgtggata aacgccgtcg ttcattccca
    29341 gcaggcaaac cactttgaac ggaattgaac gcattggcat cagagtacaa atgttaaccg
    29401 gtccggcgag aaaacgctgg ctgatacgtt cttgatccag gcgctgtgcc agttcatcac
    29461 gcaatagtga cagcggcacc gcgtcgccat actgcgcacc taaaccttcg gcgataatcg
    29521 cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga
    29581 aggcgttgag catatcgcga caaaccggca accactcttc cagcggacgc tcctgcgcca
    29641 gcccgcgacg ccagatattt agctgcatta gcagtgaagc cagatgcccc accagttctg
    29701 caattaagcc gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgcgctct
    29761 ccatcgcgta gcccagcaac atgcgcgtca ggccaaaccg ccaggtgtgt tgaccggtag
    29821 cgggaagttc cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggactcgt
    29881 tgacccactg gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca
    29941 ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa gcggctgtct ggcaacgata
    30001 acaggctgat aaacgcttca agtacaggat gcgactgccg cgcccgacgg tcggaaatgg
    30061 cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac agcctgaata aacggactgt
    30121 agctgtcgat atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc gggtcttcct
    30181 ccagcatcgc cagcaggcga tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa
    30241 cgtggaaggt gatactgcta tccagtggat caagcgggcg tttgttatcg ctacgggaaa
    30301 actcttcgat gttcacacca gcaacggcgc ggttttccag ttccagaatg tcagactgaa
    30361 tattatgcag caggttatct ggcgtcacat cgacaaaagc gtccagctcc tggctgctct
    30421 ccaggtcaga aaggagataa atgtagtcgc gcccaagctt gccccatgaa gccagcagcg
    30481 ggttgccgac atcctgttca ccatcgctgt taaagagctg cccggcattt tcgctgtcgc
    30541 gaaatagcgg taattcgcga tcttcaaaac tgtgtcggcg ctggcgagtc agtagtttcg
    30601 ccagataagc gggatcttta atgtcgcccc agtaataacg gcaggggttg gtaaacagga
    30661 gatggatttc aatatgttta cccagcgcct gtagcgcctg gagataaaca ggcggtaacg
    30721 cggaaatacc gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact
    30781 ccagcgtttc gataaagcgc tgatagagat tggcgcggtg ccagcgcggt tgcccaagtt
    30841 cgtcggtata ttccaccaac gccttccaca acggcgcttg ccaggcctgt gcttctccca
    30901 acccttctac cagatgtcct gtttcccact gtgccagcca gtccggacga tagaccagat
    30961 actggtcaaa caggtccgcc gcttttgaag aaagctggaa cagttttcgc ttgtcactat
    31021 cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg ctccaacagt tgcggcagca
    31081 gtgtcatcag tttccagctc atgctctgtt tgttaaaggc gctctctttg gggatctccg
    31141 gtaacacccg gacgaacata tcccagataa agctcgctgg cagcggaaaa tcaatgtttg
    31201 ccgcaatacc aaacttttgc gacagggtca tttgcagcca ctgtgccata ccggtacttt
    31261 gcaccagaat catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact
    31321 ccatcaacgc ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc
    31381 ctgactactg acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg
    31441 aaactagcgt aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt cggttgacct
    31501 gccagttagc aggtggcgaa atcgcgcgca gttgcgtttg ctgccagcca tgccgccaga
    31561 gctgttggta ctggtttctg ctggcaagac tgttcattaa tgttcgctgg aaacccgata
    31621 acgcagtgac aatcatcacc atcaacacca tcgccaacat tacctccggc agactaaagc
    31681 cttgttgatt cctcagggaa gctgacataa cgccctctct ttcagcggac aaaaatcgct
    31741 ccagccgcgt ggcgaaaaaa caatgtttcc atcgatgact tcgcctgttc gccacaacga
    31801 aacgccttca taaccagcaa tcaataaggc ttcattatct gccagtaaac gcagacaaac
    31861 ccgggcaccg gtggcagcat acagtaagca ctgaactgcg ggcagcgtct gccaggagtg
    31921 catttttccc cactccagcg ccgactgaac gatggcctgg cggcgcaatg actgactttc
    31981 catgctcacg cgagaagcaa aactgcgatc ctgctgactc attccttgca atagcaagct
    32041 acccaaaatc agcagcatca ggaccagagc cagtgacgaa acaccctttt cgcggttcac
    32101 aggttggatc ctgtcacgct atagctggca tccaccacgg tttgcggatc agccttgctg
    32161 gcagcgtgca tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc
    32221 tgaaaagtgt cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca
    32281 caggatgtcg caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg
    32341 tcggactctt ttaccggttc gcgatcccag ataccattac tgttcgcatc ccactgcaca
    32401 atgacacagt caccctgtcc gacaatttcc agcccttcgc ccgtacagct gccatggcaa
    32461 taacccgccc tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc
    32521 agcgccagct tacgggtgtt cgttaaactt tcacgctgta acgcaggcag aaagcgtgca
    32581 gcccccagca acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa
    32641 ccttgctctt ttacaggcat ccttctgttt ctccttgctg acaaagccgg agtcttcccc
    32701 acggcgaaac cactagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc
    32761 atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt
    32821 cgggccagcg cggcacaaag accaatggtg aactgccatg acaggtattc gccccagcaa
    32881 cggaactcac aaggcaccat aacgtcccct ccctgataag actgatactg tggtcgcggt
    32941 tatgccagtt ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt
    33001 gccaaagccg ttgcgactgc tgccagtatt gccagccata gagcccactt gcgcttagca
    33061 tgaccagaat cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgttttttca
    33121 tgccggcagt atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc
    33181 atcttccgga ctatttacgt cgttgcagga cgttgcaaaa tttcgggaag gtgtctcgaa
    33241 gaatttaacg gagggcaaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt
    33301 cgtaattaga tagccaccgg cgctttaatg cccggatgtg gatcgtatcc ttcaatctca
    33361 aagtcttcga aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc
    33421 agcggacgcg gttcgcggct taattgcaga tgagtttgat ccatatggtt gctgtacaga
    33481 tgcgtgtcgc caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc
    33541 atatgcacca gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag
    33601 gagcgctgat aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca
    33661 tggcacggtg ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg
    33721 cgacgggaat ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg gtcaatatga
    33781 cgaccatccg gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgccg
    33841 ttttcatcgg cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta
    33901 gtgtcgccct gcaggaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgttta
    33961 gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa
    34021 agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc
    34081 agttctaaat actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat
    34141 gcccagacca tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg
    34201 ctgatgtact gcacccaggc accagtaaac tgcgcgtcgg gctggcggaa aaactcaaca
    34261 atgatgcgaa acgctccgta accaatcagg aacaagcctg agacagctcc cattgggcgc
    34321 ggtttacgaa tatacaggtt gaggataata aacagcacca caccttccag cagcagctcg
    34381 taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc
    34441 gggttggttt gcagcagcaa aatatcttct gtacgggagc cagggaacag catggcaaac
    34501 gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg
    34561 gcaccaagac caaacggaat gagtggagca ataaaatcag agacctggaa gaaggaacgt
    34621 ttagtacggc gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaag
    34681 gacatgccgc cgtcccagac acggaaaaga tacagcggat cggccataaa ctgcgggaaa
    34741 ttgtagaaca gaacataacc aatacgtcca ccgaggaaga cgccgaggaa gcccgcatag
    34801 agtaagtttt caacttcatt tttggtccag ccactgcccg gacgattcgc ccgtcgtgtt
    34861 gccagccaca ttgcaaaaat gaaacccacc agatacatca gtccgtacca gtgaagcgcc
    34921 acgggtccta ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc
    34981 atctgtcacc acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc
    35041 gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg
    35101 gataccgtta aaagatatgt atatgatccg cgctataacc ccccacgaat cagcccgccc
    35161 atgccgcgac gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct
    35221 tccagactac gctgcgcaag attttctgct tcggcaaaat caatgcgccg cagcaggtat
    35281 tttacgcgcg ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg
    35341 attgccacgc acatcggatc gcccgccatt tcaccgcaca aacggagatc gattccatgt
    35401 atttccgctt cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtca
    35461 taaatgttcg ccacccgggt attgttgcga tcaacggcca ggatgtattg cgtcagatcg
    35521 ttggtgccaa cagagatgaa atcgacccgc tttgccagat gcggcagcat aaacaccatt
    35581 gacggcactt ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc
    35641 tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctt
    35701 gtgaccatcg gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc
    35761 cgcacctgga tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc
    35821 aggcatggat tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc
    35881 agcgtacgca aggtgacggg tttatcatta aacatttgca gcatcccctg atactgcgcc
    35941 acctgttctt cttccgacgg aaaaccactt tgcagcatga atgggatttc agtgcgataa
    36001 agtcctatgc catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccggca
    36061 ttgagcatga ctttaatgcg ttcgccgctt tttaactggg cgggtaaatt gacgtcatct
    36121 tccgccagac ggctaagctc gatctcttca ctaattagcc gctgatattc ttgcagcagt
    36181 accggctccg gatcgaccag caattcaccg cgataaccat cgacgatcag cgtccgacga
    36241 tgcagtaccg aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc
    36301 atgatcgcag catgggagtt ggcagcgcca tcgcgcacga caacaccgac taagcgatcc
    36361 tggggcagct cagcaagcgt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc
    36421 ggccaggcgt tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgaccaagt
    36481 gcacgtaaat cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg
    36541 gcaaattttt caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg
    36601 gcaaacaatt cgcgacgcag ccgggtatcc gaaagcaggt gtgagtaaag atcgaaaata
    36661 gccgccgttt ctttttgtgc accggcagca aagcgtttgc tatagcggcg aaactcgtta
    36721 gccgcctctt ccaacgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat
    36781 gcctgataca cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc
    36841 acaccaggtg ctgccggtaa tgcacggatt cgcgtctggc gatattgccc aaacaaggca
    36901 gtcaactgcg actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat
    36961 tcttcacttt cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac
    37021 tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag
    37081 gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc
    37141 ctgccaacca ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt
    37201 tttttcagtc cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag
    37261 accgaacaga cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc
    37321 tcattcaggc gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata
    37381 attggcgtga cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta
    37441 ttttcctgca gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca
    37501 cgtttaaatg acaccacctg tctgactgga taccagtaac ttacccatcg ccagccgtca
    37561 aactctggtg tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag
    37621 agaaaccatt tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt
    37681 ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg
    37741 cttaatccta cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg
    37801 ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc
    37861 tgcccctggc gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc
    37921 accggactac ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac
    37981 ggtaaaccac tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg
    38041 tgcagagtta taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat
    38101 agagttgtga agaactacgg aattactacg ggaaaacccg gagcattctg aataagacag
    38161 atatagagat tttaaaatat tcattatcag tcaatgtatt acatgtgatt tgagtaataa
    38221 aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc
    38281 aacgccgtat ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg
    38341 agtttcgtcg cacgtcaagg ctgtaaatgg aaacagtagt gaaggttttt cacagttatc
    38401 ccagctttct gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga
    38461 aaacccgttg cagtgttgcg caactcgatt accggcaacc taaaaagcaa tataaatcag
    38521 agaattgaac aacgcatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt
    38581 ttgtttttta accaaggtat catgacatgt cccaacctcg cccactgctc tctcctcccg
    38641 aaactgaaga acaattgtta gcgcaagcac aacaactttc tggttataca ttgggagaac
    38701 tggcggcact tgccgggctg gttacgccgg agaatttaaa acgcgataag ggctggattg
    38761 gcgtgttact ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg
    38821 ctgctctggg cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa
    38881 caacattcgt ttgtgttgcc ccgttaacgg gcaatagcgg ggtgacatgg gaaaccagcc
    38941 acgtgcgcca caagctcaaa cgcgtgctgt ggataccggt tgaaggcgag cgcagcatcc
    39001 cgctggcaaa gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc
    39061 ggcagctacg cgaagactgg gaagaattaa tggatatgat tgttctcggt cagattgagc
    39121 ggatcaccgc tcgtcacggg gaatatttac agatacgacc gaaagcagcg aatgcgaaag
    39181 cgctgaccga agctattggt gcccggggcg aacggattct gacgctgccg cgcggatttt
    39241 atttgaagaa gaatttcacc agtgcgctac tggcccgtca ttttctgatc cagtagccat
    39301 cgctttgacc tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt
    39361 ttagatgtta tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac
    39421 gctgctggag atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa
    39481 gctcccaaca gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggtcat
    39541 gcgtctggcg ctgctggcat ccatcgcctg ggttacgcgc ctgacgaatc cgctttttac
    39601 aatattcagt caggaaattt ccgcccgtga tttgattctg cttctgggtg gcttgttcct
    39661 tatctggaaa gccagcaagg aaatccacga atccatcgaa ggtgaagaag aagggctgaa
    39721 aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt
    39781 cagcctcgac tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc
    39841 tgccgtggtg attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt
    39901 cgagcgccat ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac
    39961 cctgattctg gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt
    40021 cttctctatt gcggttgaaa gcctaaacct gattcgcaac aaaaagaatc cgctctgata
    40081 ctccgtacgc tctcctgcgg gagggcgtac tcactcctgt taccctcaca gtttaagatt
    40141 ttcctgcttt caagatatat aacgtcggtt tataaacaga ctattatcat aggtgggcca
    40201 aatgaggata accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt
    40261 gctgtttccg ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg
    40321 accgatggaa aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa
    40381 ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata
    40441 aggtcagcat ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat
    40501 ttttatattc cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac
    40561 cccacagttc gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga
    40621 acagcgaagc cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta
    40681 tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga aactcaaggg ctaaccgaaa
    40741 cctacgtcgg caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca
    40801 aagtgagcgg accgtcgcgc aataatgaca aaggcatccg cccggatcag gcgctggatc
    40861 ggaagaatat ccgtgaagcg ctgcatgaca gcctcaagcg tctgcagact gattacctcg
    40921 atctctatca ggtgcactgg ccgcagcgcc caaccaactg cttcggcaaa ctcggttata
    40981 gctggacaga ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt
    41041 accaacgcgc gggaaaaatt cgttatatag gcgtgtcgaa cgaaactgca tttggcgtaa
    41101 tgcgctacct gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc
    41161 cctacagcct gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag
    41221 gggtcgaact gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca
    41281 atggtgcaaa acccgctggc gcacgtaata cgctctttag tcgcttcacc cgctatagcg
    41341 gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catagcctgg
    41401 accctgctca gatggcgctc gcatttgtac gccgtcaacc gtttgttgcc agcactctgc
    41461 tgggcgcaac cacgatggag cagctgaaaa ctaacgtcga aagtttgcat ctggagttga
    41521 gcgaagacgt attagctgaa attgaagcgg tgcatcaggt ttatacgtat cctgcaccat
    41581 aagattaacg ccg
//
LOCUS       DAOFSV010000030        41074 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.030, whole genome shotgun sequence.
ACCESSION   DAOFSV010000030 DAOFSV010000000
VERSION     DAOFSV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.030"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(85..627)
                     /gene="yfcD"
                     /locus_tag="QBH27_001680"
     CDS             complement(85..627)
                     /gene="yfcD"
                     /locus_tag="QBH27_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDQ7014002.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(685..1236)
                     /gene="yfcE"
                     /locus_tag="QBH27_001681"
     CDS             complement(685..1236)
                     /gene="yfcE"
                     /locus_tag="QBH27_001681"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDQ7014003.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(1292..1936)
                     /gene="yfcF"
                     /locus_tag="QBH27_001682"
     CDS             complement(1292..1936)
                     /gene="yfcF"
                     /locus_tag="QBH27_001682"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDQ7014004.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            2072..2719
                     /gene="yfcG"
                     /locus_tag="QBH27_001683"
     CDS             2072..2719
                     /gene="yfcG"
                     /locus_tag="QBH27_001683"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDQ7014005.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLNHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            2776..3138
                     /gene="folX"
                     /locus_tag="QBH27_001684"
     CDS             2776..3138
                     /gene="folX"
                     /locus_tag="QBH27_001684"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDQ7014006.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYTDSVSMTLSWQR"
     gene            3159..4052
                     /locus_tag="QBH27_001685"
     CDS             3159..4052
                     /locus_tag="QBH27_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDQ7014007.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHERKERLCQSRWNTTQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPTILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(4211..4984)
                     /gene="hisP"
                     /locus_tag="QBH27_001686"
     CDS             complement(4211..4984)
                     /gene="hisP"
                     /locus_tag="QBH27_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDQ7014008.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSDGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(4992..5708)
                     /locus_tag="QBH27_001687"
     CDS             complement(4992..5708)
                     /locus_tag="QBH27_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7014009.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(5705..6391)
                     /gene="hisQ"
                     /locus_tag="QBH27_001688"
     CDS             complement(5705..6391)
                     /gene="hisQ"
                     /locus_tag="QBH27_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDQ7014010.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(6481..7263)
                     /gene="hisJ"
                     /locus_tag="QBH27_001689"
     CDS             complement(6481..7263)
                     /gene="hisJ"
                     /locus_tag="QBH27_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDQ7014011.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(7484..8266)
                     /gene="argT"
                     /locus_tag="QBH27_001690"
     CDS             complement(7484..8266)
                     /gene="argT"
                     /locus_tag="QBH27_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDQ7014012.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(8532..9101)
                     /gene="ubiX"
                     /locus_tag="QBH27_001691"
     CDS             complement(8532..9101)
                     /gene="ubiX"
                     /locus_tag="QBH27_001691"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDQ7014013.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(9196..10713)
                     /gene="purF"
                     /locus_tag="QBH27_001692"
     CDS             complement(9196..10713)
                     /gene="purF"
                     /locus_tag="QBH27_001692"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDQ7014014.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVVMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(10750..11238)
                     /gene="cvpA"
                     /locus_tag="QBH27_001693"
     CDS             complement(10750..11238)
                     /gene="cvpA"
                     /locus_tag="QBH27_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDQ7014015.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(11679..12341)
                     /gene="dedD"
                     /locus_tag="QBH27_001694"
     CDS             complement(11679..12341)
                     /gene="dedD"
                     /locus_tag="QBH27_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDQ7014016.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(12331..13599)
                     /gene="folC"
                     /locus_tag="QBH27_001695"
     CDS             complement(12331..13599)
                     /gene="folC"
                     /locus_tag="QBH27_001695"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDQ7014017.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVGQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(13669..14583)
                     /gene="accD"
                     /locus_tag="QBH27_001696"
     CDS             complement(13669..14583)
                     /gene="accD"
                     /locus_tag="QBH27_001696"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDQ7014018.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(14739..15398)
                     /locus_tag="QBH27_001697"
     CDS             complement(14739..15398)
                     /locus_tag="QBH27_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ7014019.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(15481..16293)
                     /gene="truA"
                     /locus_tag="QBH27_001698"
     CDS             complement(15481..16293)
                     /gene="truA"
                     /locus_tag="QBH27_001698"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDQ7014020.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(16293..17306)
                     /locus_tag="QBH27_001699"
     CDS             complement(16293..17306)
                     /locus_tag="QBH27_001699"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDQ7014021.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(17372..18508)
                     /gene="pdxB"
                     /locus_tag="QBH27_001700"
     CDS             complement(17372..18508)
                     /gene="pdxB"
                     /locus_tag="QBH27_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDQ7014022.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     LIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            18607..19602
                     /gene="flk"
                     /locus_tag="QBH27_001701"
     CDS             18607..19602
                     /gene="flk"
                     /locus_tag="QBH27_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDQ7014023.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDISLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(19599..20777)
                     /locus_tag="QBH27_001702"
     CDS             complement(19599..20777)
                     /locus_tag="QBH27_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDQ7014024.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(21042..22262)
                     /gene="fabB"
                     /locus_tag="QBH27_001703"
     CDS             complement(21042..22262)
                     /gene="fabB"
                     /locus_tag="QBH27_001703"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDQ7014025.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22421..24427
                     /gene="mnmC"
                     /locus_tag="QBH27_001704"
     CDS             22421..24427
                     /gene="mnmC"
                     /locus_tag="QBH27_001704"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDQ7014026.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAYPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMELTLPLPCSTPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSLVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(24548..24826)
                     /locus_tag="QBH27_001705"
     CDS             complement(24548..24826)
                     /locus_tag="QBH27_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDQ7014027.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(24860..25408)
                     /gene="epmC"
                     /locus_tag="QBH27_001706"
     CDS             complement(24860..25408)
                     /gene="epmC"
                     /locus_tag="QBH27_001706"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDQ7014028.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(25408..26217)
                     /locus_tag="QBH27_001707"
     CDS             complement(25408..26217)
                     /locus_tag="QBH27_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDQ7014029.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(26217..27041)
                     /gene="mepA"
                     /locus_tag="QBH27_001708"
     CDS             complement(26217..27041)
                     /gene="mepA"
                     /locus_tag="QBH27_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDQ7014030.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IIGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(27045..28130)
                     /gene="aroC"
                     /locus_tag="QBH27_001709"
     CDS             complement(27045..28130)
                     /gene="aroC"
                     /locus_tag="QBH27_001709"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDQ7014031.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTEIPRW"
     gene            complement(28165..29097)
                     /gene="prmB"
                     /locus_tag="QBH27_001710"
     CDS             complement(28165..29097)
                     /gene="prmB"
                     /locus_tag="QBH27_001710"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDQ7014032.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNRAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            29263..29814
                     /gene="smrB"
                     /locus_tag="QBH27_001711"
     CDS             29263..29814
                     /gene="smrB"
                     /locus_tag="QBH27_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDQ7014033.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(29874..30698)
                     /locus_tag="QBH27_001712"
     CDS             complement(29874..30698)
                     /locus_tag="QBH27_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDQ7014034.1"
                     /translation="MKKMSGLFFLLLTGLLSATAWADVTFRDVKTYTGFWGNGATTKV
                     VFRIDVLTPEGVYYGEYAQDGYTLWLGNIPNTVWTGPGSAPTMNITHQSNTVDNSNCP
                     GITDGWSCVDLGVDIIVHGETHGCPWLVSTNTVSTAARGYTYIGPSAKQTRCPTEPVG
                     PYDISWNENYVLHNKMLTLQSTGGVITQTLSTYLMRDGRVCDGSRMDDRGAYCRFMSY
                     MITFSTSGCDDSKVTVTPVAHPITDKQLHDMQIRIDTSSQQPIDATCRFQYILNEL"
     gene            complement(30700..31227)
                     /locus_tag="QBH27_001713"
     CDS             complement(30700..31227)
                     /locus_tag="QBH27_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014035.1"
                     /translation="MSLKRIFHCAAALAAVLLTPNVQAADNLHFYGNLLSKSCTLVVQ
                     GEVLAEVHFPTVSRRDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDAASTAQGVGIGMEKTDGTAVLINNTSGATFVLSSGNNDLHFNAWLQAKSGRDVT
                     MGDFTASLTATFEYI"
     gene            complement(31224..31703)
                     /locus_tag="QBH27_001714"
     CDS             complement(31224..31703)
                     /locus_tag="QBH27_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014036.1"
                     /translation="MKRFALCLFGCYCGIAQAADSDITFHGTLVSPPACAISDGKTVE
                     VEFRDVIIDNINGNNFRQNVPYTITCDPDVRDDAWEMTLSWTGSQTSYDNAAIETNVT
                     GLGIELQQNGQPFTLGTPLKINLSTPPTLQAVPVKASDASLSEGTFSAWATLQVDYQ"
     gene            complement(31700..32191)
                     /locus_tag="QBH27_001715"
     CDS             complement(31700..32191)
                     /locus_tag="QBH27_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014037.1"
                     /translation="MKGVLLPVAGMVLLACGSAWADSKTVNLTLRILVDAPPPCTVKG
                     SAVEFGNVIIKNIDGSNYRQPVGYTLNCSNSVSDDLQMQLQGTTTTINGETVLSTGIN
                     GFGIRIQDASSHAPVALAGNNWLPFNVNSRPGFEAVPVKQSGTQLTASEFSATMTMVV
                     EYQ"
     gene            complement(32208..32960)
                     /locus_tag="QBH27_001716"
     CDS             complement(32208..32960)
                     /locus_tag="QBH27_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ7014038.1"
                     /translation="MFYKLSCSMVVSTTLAVLLTTTNLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYIVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMNSAPV
                     LTYINDYGARLPLVFRCEGDTCKVDEAQSLKG"
     gene            complement(32980..35628)
                     /locus_tag="QBH27_001717"
     CDS             complement(32980..35628)
                     /locus_tag="QBH27_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ7014039.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQSLSDEYDINWYVSENDPTKTYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGISGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDEDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDASQSDMARTGNDKE
                     MYTITYNQNFAAAGVSVYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNTDKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFNRIDDATHYQINVG
                     TSEQHGSVDGYLSHDGTLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGINAGEHMMVFWEGSAQCEIVLPKPLPADLFRGLLLPCEQKGTTAPDSLAP
                     EIKPVIQDQTRQVTPSEAPTSISATQ"
     gene            complement(35709..36275)
                     /locus_tag="QBH27_001718"
     CDS             complement(35709..36275)
                     /locus_tag="QBH27_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014040.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIHLQDCVFTTESNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
     gene            complement(36834..37319)
                     /gene="sixA"
                     /locus_tag="QBH27_001719"
     CDS             complement(36834..37319)
                     /gene="sixA"
                     /locus_tag="QBH27_001719"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDQ7014041.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTHGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
     gene            complement(37522..39666)
                     /gene="fadJ"
                     /locus_tag="QBH27_001720"
     CDS             complement(37522..39666)
                     /gene="fadJ"
                     /locus_tag="QBH27_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDQ7014042.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKFSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKMPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSASANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            complement(39666..40976)
                     /gene="fadI"
                     /locus_tag="QBH27_001721"
     CDS             complement(39666..40976)
                     /gene="fadI"
                     /locus_tag="QBH27_001721"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDQ7014043.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
BASE COUNT         9927 a        11242 c        10254 g         9651 t
ORIGIN      
        1 tattatgtcc agcgtcgtgg tgatcggcgc tcagttgatg ggctaccact aaaaatgtta
       61 agagccgcga ttgcggctct tttttcattc tgccgtttca gtctcgacgg cttcattttt
      121 ggcattgcgc ttcatccaca acgccagcgc tttcagcgag tctggagtga actcatcgca
      181 gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca ctgacttcat cttcctgtag
      241 agcgaaggga ccgtgagaga cgcagctgaa caacgcgccc cagacacggc aatttttatc
      301 ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc
      361 cgcttcgcga cgcgcggatt ccagcagctg ctcatcggcc tggactacac cgcctgcggt
      421 cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt
      481 gcccatgcca tcatgcacga cgatataagt tgcacgatga cgcagacact gtgcccgcat
      541 ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc
      601 agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac
      661 ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg
      721 atcattgagt gcgattacac taagtacgtc attatccagc atgccataac tcgccggatt
      781 accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc
      841 tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggtag
      901 attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac
      961 ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt tgatccactt cgctgtcgca
     1021 gttgccgcgc acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc
     1081 tggcgcgtaa ccctccggta aagcattacg cgggccatga ttcaacacgt cgccaaggat
     1141 caccagccac tgggcaccgc tttgggcaaa caactccaga acacgttccg tcgccggtaa
     1201 cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg
     1261 taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa
     1321 taaaacgctg gacagacgct cgctgccact ggaatgtcgc ataatccacc aggcgttccg
     1381 gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa
     1441 tgcaccattc accaaataaa ttcggctgac ccagtgctaa caaatgctct gccatcgcga
     1501 acagtttctc tgcactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa
     1561 agacaacatc cgtcgggcgt tcttcgcgga tgggcatcag atcgctgcgc agccaggcct
     1621 gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg
     1681 gtggcgcaaa tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat
     1741 catcgatttg taataacggc acacggcgtg tctgaccgta accttgccac gtcggctgca
     1801 aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttctcctgca
     1861 acgccaccca ggcggataac acataagggg agaaaaaatg ggcatctgac caaagcgtaa
     1921 tagcgggttt actcattatg tcctcggcaa taaagggggc ttgctatcaa actatagagc
     1981 ctttttgtca ctgtcacgtg acgaatactc acgcaagtcg ccgcgctttc tatacttgtg
     2041 gtgttcagat gattagacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc
     2101 caatggtcac aaaattacgc tgtttctcga agaagcaggg ctggattatc gcttgataaa
     2161 ggtagacctg gggaaaggag gtcagtttcg cccggaattt ttgcgcattt cgcctaacaa
     2221 caaaattccg gcaattgttg atcattctcc agccgatggc ggcgaaccgc taagcctctt
     2281 tgagtctggt gccattttgt tgtatctggc tgagaaaacg ggactctttt tgaatcatga
     2341 aacgcgtgaa cgcgccgtca cattacagtg gttattctgg caggtaggcg gactggggcc
     2401 gatgcttggg caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat
     2461 tgaacgttat caggttgaaa ctcagcgtct gtaccatgta ctgaacaagc ggctggaaaa
     2521 ttcgccctgg ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt
     2581 taatgcctgg actcgccagc gaattgacct cgcaatgtat ccggcagtca agaactggca
     2641 tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg ctaaaagcac aacacggtga
     2701 tgagcgttcg gatagttaac agaaacaggt tctcgtgtat tatttcattc taagtaaaac
     2761 aacggagaac ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgttta
     2821 cgtacgttta tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat
     2881 gtgacgatcc actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg
     2941 aattatcgca ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg
     3001 ctggaaaaat taactcagga tgtgctcgat atcgcacgcg aacatcactg ggtgacgtat
     3061 gctgaagtgg agatcgataa actgcacgcg ctgcgctaca ccgattcggt atccatgacc
     3121 ttaagctggc agcgttaacc accatatcgg gaggctgcat gaacatagtg atcaccggag
     3181 ggacgggatt aattggtcgc catttgattc cacgtttgct ggacctgggc catcaaatca
     3241 cggtagtgac gcgtaacccg cagaaagcca gttccgttct cggcccccga gtgacactat
     3301 ggcaagggct tgccgaacaa agcaacctca acggcgttga tgcggtaatc aacctggccg
     3361 gagaaccgat tgctgataaa cgctggactc acgagcgaaa agagcgtctc tgccaaagcc
     3421 gctggaatac cacgcaaaaa ctggtcgatt tgattaatgc cagcgacacg ccaccgtcgg
     3481 tactcatttc cggctcggca acgggctatt atggcgactt aggtgaagtg gtggttaccg
     3541 aagaggaacc gccgcataac gaatttaccc ataaactctg cgcccgctgg gaagagattg
     3601 cctgtcgggc gcaaagtgac aaaacgcgag tgtgcctgct gcgtaccggc gtagtgctgg
     3661 caccagacgg tggtatcctc ggtaaaatgc tgccgccatt tcgttttggc ctgggcgggc
     3721 cgattggctc cggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggca
     3781 ttctctggct gctggataac gagctgcgcg ggccatttaa tatggtttcg ccctacccgg
     3841 tacgcaatga acaatttgcc catgcgctcg gtcatgcgct gcatcgcccg accattttgc
     3901 gcgtccccgc gaccgccatt cgactgttaa tgggcgaatc ttcagtactg gtattaggtg
     3961 gacaacgcgc gctgccaaaa aagctggaag aagcgggttt tgcgtttcgc tggtacgatt
     4021 tagaagaggc gctggcggat gtcgttcgct gatgtggttt actgcaaaca tccgccagcg
     4081 gaaacgctga tgaataaata tagataattc agaactattt ttagcggact gcaactttta
     4141 catttcagcg gaagcggact cgcaaattcc ccatgaaata ctccggcagc gttaaccacc
     4201 ggagaggaaa ttatttcagc gaacctttaa ggaactgttg cagacgaggg ctttgcgggt
     4261 taccgaataa ctgctccggc gccccttctt cttctatttt cccctgatgg aggaaaatga
     4321 cgtgagtaga aacatgacga gcaaagccca tttcgtgagt cactaccacc atggttttcc
     4381 cctcttctgc cagttgctgc ataatacgca acacttcgcc taccagttca ggatcgagcg
     4441 ctgaagtagg ttcatcaaac agtaaaactt ccggttccat cgctagcgcc cgcgcgatag
     4501 aaacacgctg ttgctgacca ccggacaggt gcaccgggta tttcccctgc gcacgttcgt
     4561 ctatcccgac ttttgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
     4621 ccaacacctg aatcggcgct tccatgacgt tttccagcac cgtcatatgg ctccagagat
     4681 tgaagtgctg aaataccatc gtcaggcgtg tgcgcaataa gcgcagctga tttttatcgg
     4741 cgactttgag ttgaccatct ttatcgcgca ccagattgat cgtctggcca ttgaccacga
     4801 tcgatccatc gctcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
     4861 atcccgacga tccgatgatg cttatgacat ctccggcatt cgcttgcaat gagacccctt
     4921 tcagcacttc gtgttcgccg tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
     4981 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttcagct
     5041 ctacgaaaga ggctgatcag aacataagag atgattaaat agagcaccgc ggcaatgccg
     5101 aaagcggtaa aaggttgata tgtggcggcg ttaatatcgc gggcaatttt cagcagatcc
     5161 ggcaccgtgg cagtaaatgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg
     5221 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
     5281 gtcgagaagc cataggctct ggcggcttca atttccccat gcggcactga acggattgcc
     5341 ccggcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
     5401 ttcaggccac tgcgaaaaaa ggcgttaagg aactcggttc ccttaacaat ctccagcgtg
     5461 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
     5521 gtaaataacc agattggaaa ctggatgtat ttattacttg agacgcgacc aatcgccaga
     5581 aacagcgcca gaactccgcc tatcactacc gacaaaatca gcagccacag ggtgatcgcc
     5641 acgccagtaa agcgataacc gtcagtccac agcagtggtt tccagtattc atgtaagatt
     5701 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
     5761 caccattgga aacggtggtg aaaacaagat aaatcacgcc gcagacgata gcgaaataga
     5821 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
     5881 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
     5941 gggcgtaacg catcatcgcc ggaaacatga tccgccgaaa cacttgccca cgagtaaaac
     6001 cgaacgccgt cgccgcctct atatgtcctt tcggcactgc cataaacgcg ccacgaaacg
     6061 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aatacctgcg accatcggat
     6121 caatatcaat ctgcccgacg cccatcgcct ccgtcaccgt gtttagcgca atctgcaaac
     6181 cgtagaaaat cagcagcatc aatactaaat ccggcacgcc tcgaatcagc gtggtgtacc
     6241 cttcgaaaat aaggcccgaa agccgatttt gtgagagctt accgccagcg ccaattaaac
     6301 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
     6361 gtaaaataac acttgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
     6421 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
     6481 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
     6541 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
     6601 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg ggccaccgaa
     6661 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggctgcga cctcatcctg
     6721 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
     6781 agaaacgatt tcaatgcctt ttggtgccca gtgttcgtta ccgaacgtct cctgggtggt
     6841 gccctgcaat acgcctaccc gtttgccttt cagcgattcg actgtcggct gaatgtcgga
     6901 atttttcgcc accaccagac gagaatcggc tgcgtacagt ttgtcggtga aggctatttc
     6961 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
     7021 taaggacggg attaacgcat ccagtgggtt ttcgacaaac gtacattgcg tattgatgcg
     7081 tttgcacaac tccttggcca gatcgatatc gaagccaacc agttcacctt gtgaattttt
     7141 tgattcaaat ggcgcatagg tcggatcggt accgatgcgg atgttttgcg gaatcgcagc
     7201 aaatgctgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
     7261 cataatgtcc tcaactgaca gtctttttat cgggaatatt tacaggtttg ttacacttat
     7321 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgctagacgt attatttttc
     7381 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
     7441 accatgatga tcattccatc aggtacagct tccctgcgac gtatcagtca ccgtagacat
     7501 taaagtcaaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
     7561 gcgccttatt aaacgcagcc gtcagttccg catcatcttt acgtagccct acaccggtgc
     7621 cgtcaccgaa gtattttttg tctttcactg atgggcctgc aaaggcgaaa tctttaccgg
     7681 cgggttgctt aaggaaacct tcgctggcag caacttcatc ttgtaacgca gcatccagac
     7741 gtcctgcagc cagatcggaa tagactaaat cctggttggc ataggccacc acatccacgc
     7801 ctttgctacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
     7861 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcggcaatca
     7921 aacgagaatc tgcggcgtac agcttgtcgg agaaagcaat ctcctgctga cgtttatcgg
     7981 taatggagag cgacgaaata atggcgtcga tttttttcgc tttcagtgag gggatcagcg
     8041 cgtcaaagtc gctggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac
     8101 cgagatcgat atcaaagcca acaaagtcac ctttggcatc tttcgatgag aacggtgcgt
     8161 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
     8221 cggagagacc gactaacaaa gacagagcga gaatcgactt cttcatacat aaccctcaag
     8281 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
     8341 tcttatgcca tcttgacgac agcagtaaca ttcaacgtta aatatgttaa taagaagttg
     8401 cattattgtc ctaaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
     8461 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
     8521 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttaccgca
     8581 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgtgggcga
     8641 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
     8701 aaacgcagat ggcctaagtg cagtggcgtt tcacgcacgc agagcaccaa cgggcgacgc
     8761 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
     8821 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccaacgtctg gaaagaaccg
     8881 gaagaaatgc tggcagcaat atcgcgcgca tcgtgtgtca catcagctaa cgcctgcact
     8941 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcattacc
     9001 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataaacgcac gccataaatc
     9061 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
     9121 cttgcaggca gactttgcag gatttcggcg ggagttgcaa gtcagggtgc cagaccggca
     9181 ccctgagcga aggcatcatc cttcgttatg catttcgaga ttttccactt cgttctgacg
     9241 ttgcactgct ttggcgtcgt cattacgtaa agtatcgagg aaatcgaggt agccctgatc
     9301 aacgtctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
     9361 gttttcagcg cgaacggctt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
     9421 accgatgatc tggcgaattt catctacttc gcgaccgtga gcgatcagtt ccgtggcgct
     9481 cggcatatcg ataccgtaaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
     9541 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
     9601 gacgatagag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcgtt
     9661 cagtttacgg cgcacggact tacgacgcag ctgctgaccc ggcatgatga aggtgcggcc
     9721 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gcgcaatttc
     9781 cagcgcgata tcgcaggaag tttccgggat cgggatcacc acgtcgatat ccagatcttc
     9841 ccattcgcgg gcaattttct cgcccagctt cgtgcccata ttcacacgcg cgctgtaaac
     9901 ggaaattttg tcgatgaacg agtccgggcg agcaaagtat acatactcaa acaggcacgg
     9961 attgctgacc ggattgtcag cacactgacg ggtaaacaac tgcccttctt cagtgatgta
    10021 aatcgcttcg ccaggggcga catcacgcag gaactcaaag cccagcgtat cgagcgcaac
    10081 gctttcggaa gcgaccatat attctgtgcg attatcgtca atatcacgtt tgcccagtac
    10141 aagcggacga atcccgttag gatcgcggaa agcaaccata ccgtggccga taatcatcac
    10201 cacacaggca tacgcgccgc ggattaagcg gtttgtagca gcgatggcgg cgaaaatgtt
    10261 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    10321 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    10381 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggagagtt
    10441 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    10501 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    10561 cagcccgttc gctttccgca aacggaagca gttattggca tctatggtga tgatgccggc
    10621 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    10681 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    10741 tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca
    10801 tctgatgata aaactgaact gcgggatcag ctgtgatttg ctccagtctt cgcttttcga
    10861 caccccggta aaggagtcga gaaagaagag aatggcagca acaatcaaca caccgcgcaa
    10921 ggcaccgaaa cagacgccca acacccgatc ggtgcctgac aaccccgttt tctccaccaa
    10981 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    11041 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    11101 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    11161 acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    11221 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    11281 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    11341 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgggcaatg
    11401 cgtgtcggat gcggcgtgaa cgccttatcc gacctacggc tctacccctg cacaggcctg
    11461 ataagacgca ctggcgtcac atcaggcaac ggctgtcgga tgcagcgtga acgccttatc
    11521 cgacctacgg ttctacccct gcacaggcct gataagacgc gctggcgtcg catcaggcaa
    11581 cggctgtcgg atgcggcgtg aacgccttat ccgacctacg gttctacccc tgcacaggcc
    11641 tgataagatg cgccagcgtc gcatcaggca aaaccgtatt aattcggtgt atagcccatt
    11701 accacaccac ttaagccaga aagctgcttg agctcaccca gcgatccttt cagcttctct
    11761 ttggaagcat ctggcccaac cagaatacgg gtaattttac cctgcactgg cgtggatggc
    11821 gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt cactttatcg
    11881 gcatttttca gcgcgcccag ttgcacaaca taggctttac ccgttggcgc tgctttttct
    11941 tccacgaccg gcttcggttc tggcttcggc gcaggtggtg cttcaacctt cggtttaggc
    12001 gactccaccg gtttcggctt cggtgggacg accggtgcag gttccggttc aaactcggtg
    12061 ttattggctg caatagtggc cggatcgagc gacggtgctg cggcatcgcc tgcccgcacc
    12121 tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct gggtggcggc tggcatcata
    12181 tcaggctcat cgcgatcgcc cgctttcggc accagcggga tagccgcgaa ctcatcctga
    12241 taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag cgccaccagc
    12301 acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat
    12361 cacttccatg acatgtgcga ccgtgtggaa cgaaccacac accagcacgg tgtcttccgc
    12421 tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt
    12481 acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca
    12541 ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat ctttatcatg
    12601 tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgctttca tacgcccggt
    12661 gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct
    12721 cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc
    12781 actgacctcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg
    12841 gacaagcggc aacggcagat tttccagcgt gccgtgagca tcgctaaacg tccagtcatg
    12901 atcggtgacg gaatagttcc actcaacgcc ccgacgttgt agcagtgcac ctttttcctg
    12961 ccccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg gtttttcgct
    13021 gcgaaagata cctgctttct cgcgaccaat actttcgcga tctggcccca gccagtcggt
    13081 atgatccagc gcaatactgg ttactaccgc gacatcggca tcgacaatat tggttgcgtc
    13141 cagacgaccg cccagcccta cttccagaat cactacgtca agttgtgcct gcttgaacag
    13201 ccacaatgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga
    13261 ttcaatctcc gcaaaagaag cggtgtgggc cgattccggc aattcctgcc cctgcacacg
    13321 tacgcgctcg gtataacgca ccagatgcgg cgaactgtag acgcccactt tgtaccctgc
    13381 cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg tacccgcaac
    13441 ggtaaacaca aatggcgctg gtttcagaac gccaagacgc gccgcgacct ggctcacgcg
    13501 ctcaaggccg agatcgatag ttttactgtg caggttttcc agataagaaa gccacgaagc
    13561 cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg attcgttacg
    13621 gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc aggcctcagg
    13681 ttcctgatcc ggtaccggtg gcaccactac gccttcacgc ggcgcttcag gattcggcgc
    13741 tggcagattc atcaacttcg ccagaatgct cgccagtttc agacgcattt ccggacgacg
    13801 gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga atcctggcgg
    13861 cagtttttcg cgaacagtct gttcgataac acgcggaccg gcaaagccaa ttaacgcttt
    13921 cggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac cgcccatggt
    13981 cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc
    14041 agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga
    14101 ggcggagaag cagatcagtg ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa
    14161 acgcgcacct accacagacc ccatggaacc gcccataaag gcgaactcga atgccgcagc
    14221 gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt tttcgccggt
    14281 ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa acttcagcac
    14341 atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta acaggctatg
    14401 caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga cctcaagatt
    14461 acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag tccacacccc
    14521 ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt caatccagct
    14581 cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg gggacgcata
    14641 atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa cagcccgaaa
    14701 ctttggataa aaaagtggtc gaaccgcaga gttacttttt attttgcggc gcgtgccgct
    14761 gcgcgtttgt ggcgaataaa ttcgaaaaca cctggcaata ttgaaacaaa aataatgcca
    14821 acaatcatca gtttaaggtt gctctgaatg aaggggagcg tgccgaagaa atagcctgcg
    14881 taggtaaaaa gcagtaccca caacagtgcg ccaatcacgt tataagcggc gaaatgacgg
    14941 tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa
    15001 cgggcgagaa taatcgtttt gccgccatgt ttctcataaa actgatgggt tttgtcgaga
    15061 taactgcgac ggaaaatttt cgaattggga ttactgaata acttttcacc gaacaaccgc
    15121 ccgatagtgt aattgaccgc gtcacccaca atcgcggcga tcagcatcag caccaccatc
    15181 atatggacgt tgagatcgtt ggtctccagt gatgccaacg ctccggcaac aaacagcaat
    15241 gaatcacccg gtaaaaacgg cgttaccacc agaccggttt cacagaacaa aattaagaac
    15301 aaaatggcat aaacccagac gccgtactcc gcgaccagct ctgccagatg cacgtcaatg
    15361 tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc cttgtactcg
    15421 tcatctatca ggctgtaact acgttggcta cagcctgaaa tgttccgagc attattctcg
    15481 ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatatc ggtcagggta
    15541 atccaccgcg accagatata gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc
    15601 ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac cgacttccat
    15661 caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt tgatatctac
    15721 caccacataa ggaccgtgac gcgtgacatt aatatgcata acgttgcgcc acggggtccg
    15781 ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca aacattgcgc
    15841 agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag
    15901 taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag ccgtggcgct
    15961 aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga tgtcaccagg
    16021 taaattcgca tttacgccca acgtccacgc agcgtctttg cgctgcgcgg tcgtttcgaa
    16081 atgcacaacc tgcccggtac cgtgtacacc ggcgtcagta cgcccggcgc agaagacggt
    16141 gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca cactgcggac
    16201 ttcattctgc cgttgccagc cgtaatacct actgccgtcg tactcaatgc ccagcgcaat
    16261 tttataaact ggcggttgtt gctggtcgga cattagtaca gatactcctg caccagtttc
    16321 tcggcaattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag
    16381 aactgaactt gctccggcat accgtagtca ttacgtacgc agccaacaga aagatgcggc
    16441 gtaccagaag catcccctac ctgagtcggg aattcgttct cttcagagag cacaatatct
    16501 tcgccctgag caaacgcatc acgcgcttct tctgccgcca gcggacgcag tgcttcaaag
    16561 ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc
    16621 agcccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg
    16681 ctaccttcgc tatccggcag taacggcagc atgttgaacg ccagctgacg cccgaagaaa
    16741 tcttcttcgt caatcggaat gccattgagt aatttagcac tctgccccgc taacgcatcg
    16801 accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacgctgat acgtgataaa
    16861 ccgccctgat caattagcgg tttcagtgcc gccagcagct ggctggtcag actgtctggt
    16921 acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac ttccggcacc
    16981 accagcggta cgtcgggttc gagagcaaac aatccgctgc tgtcgatcac cagacaacct
    17041 gagttggtcg cttcttcaac ccaggcagcg gtagcctctt tgcctgcgac aaaaaatgcc
    17101 agctgcgcct gcgtccagtc gaattcagca gcatcctgca cggtgattgt cttaccacca
    17161 aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc cccaaccggg
    17221 aactgacgtt cagccagcgt ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg
    17281 acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa agctccctgc
    17341 caatattaca gcagagagca tgaagaagag attaacgtgc cggatgatga acggcgttaa
    17401 aacccagttt acacagcaat gatgccgcgc tggcgtcatc acaaatcaca tacagagatg
    17461 accattcacg gcgctcaaga tagtttttgc gcagcttatc gaactcaccc ggtattccgg
    17521 cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc accagccttt
    17581 ttagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg gccaaactct ggcgcgggca
    17641 gcaacgtatc cagcgcaacg tgttgttcat gcccaataag cttgctatag gcttcaaaca
    17701 cttgcgtagt accgcgtgct ttaccttcca gggtatagcc tgcgatatgc ggtgtgccga
    17761 tatccacttt tttcagcaat tccacgttga gttccggttc gccttcccag acatccagca
    17821 ctacgcttaa cttctggcct tcgttcagac aggtcaatag ggcagtatta tcgacgactg
    17881 cgccacggca ggcgttaatc agaatcgctc ctggtttcag gctacggatc agtttttcat
    17941 ccgccagatg cagcgttttg tacggcccgt ctttgaagag tggcgtatgg aaagtcagaa
    18001 tatccgcatg ctgaactaat tcatccagcg agcggaaatc cccctcatcc ccgcggtcgg
    18061 cgcgaggcgg atcgcaaagc aaggttgtaa tccctaacgc ttccagtcgc gcctgtaaac
    18121 ggcgaccaac gttaccaacg cccacgatcc ccactgtacg gtcgtgcagt gaaaatccat
    18181 cgcgttcggc aagcatcagc agtgaggaga aaacatattc caccaccgca atcgcattac
    18241 agccaggtgc agcggaaaaa ccaattcccg cctgctttaa ccatgcttca tcgacatggt
    18301 cggtccccgc ggtggcagtg ccaacaaatt taatgggttt tcctgccagc aaagattcat
    18361 tcactttcgt gaccgaacgc accataagcg catccgcgtc tgccagttga gcgacaggaa
    18421 ttggacgccc gggaaccgcg gtcacctcac ccaaacggct aaataagtcg cgggcataag
    18481 gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac gagagttaac
    18541 cagacaagtg tgccataatc tcgcggccag gcatacttgc gaagatttca ggtataagga
    18601 tacgtaatga tacaacctat ttccggccct cctcctgggc aaccaccagg ccagggagat
    18661 aacctgccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac ttcgctggaa
    18721 agcttaatga cgaaagtgac ctcactgacg caacaacaaa gagcagaact gtgggcgggt
    18781 atcaggcacg atattagtct gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc
    18841 gctgagcaca atctggcgca acgtctgctg gccgcgcaaa aaagccattc tgcccgccag
    18901 cttttagcgc aattagggga gtatttacgt ctggggaata atcgtcaggc ggtcacggat
    18961 tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga gcaattaaag
    19021 accattctca ccctgttgca ggaagggaag atggttattc cgcaacctca gcagcgcgag
    19081 gcgaccgacc gtccgttatt accggcggag cacaatgcgc tcaaacagct ggtgaccaaa
    19141 cttgcggcgg caacgggaga acccagcaag cagatctggc aatcgatgct ggaactttcc
    19201 ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt gacctggcta
    19261 caggcgcgtc agacgctaag ccagcaaaat acgccgacgc tggaatcact ccagatggcg
    19321 ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat ccagcaaaaa
    19381 tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat tcttaatcag
    19441 ctttatcaac ggcgggttaa agggattgag ccgcgtgata tgcaaccgct gcttaatcct
    19501 tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct gtggatactg
    19561 ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt agccccgacg aaatgacagt
    19621 atcgtgacaa taattcccag caccgcagag atcgccccgg caagaaatac cgaagagtaa
    19681 ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga cgccgagggc gatatcctga
    19741 aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc
    19801 tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc cgcgcccgct
    19861 aatgcgaccc acgcaccagg ggcttgccag agcagcaaca agcccaccgt ttctacaagc
    19921 agagagacga tcgccacttt cacgccgcca aaacggtccg gcatccagcc aaacatgacg
    19981 cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat cgcccatcct
    20041 ttactggcaa agtagagcga aacgaaagtc ccgataaccg caaaaccaac gccttgtagt
    20101 gccagaccta accctggttt ccagataagc ccgacaacgc tccacagcga tggacgttct
    20161 cccgccaggg ccggtacttt gcgcactgtg ccgttacagg cccacgccag taagggtaat
    20221 gccattgtgg tgagcgccag tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc
    20281 ggcgcaccaa cagcgagggc accgtaaatc gccattccgt tccatgacat cactttgccg
    20341 gaatgttttg gcccaacgat gcctaatccc caggtcagag cgcccgtcag taactggctt
    20401 tcaccaaagc caagaatcaa acgcccgacg accaacaggg caaatttgaa cggtgcggag
    20461 acgggcaaaa tcgccgccaa cagcagcgcg ccgccagcca gaccacaagc taacatcccc
    20521 tgaagcgccg aacgttttgc gccatattga tcggccagtc gcccggcgta accacgcgtc
    20581 agcaccgtag ccagaaactg aatgccgacg gcaataccga ccatggtgtt gccatagccc
    20641 agttcatgat gaacaaacag cgggataacc ggcaacggca gccctacggt catgtaggtg
    20701 agaaaaaccg caaaagcgat gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt
    20761 tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggaa tttccccgtc
    20821 tcatctctct ggtttcaggg ttacggtgcg ttggcaggat ttaacgcgta cgtcttttca
    20881 gaaggaaatc gacaaagcgg gaagtttgcc tggaacaggc ggcgattgtc aataatgtga
    20941 aaaagggaac catcaggttc ccttttgcgt tagtgtcgga tgccgcattg gcgcgtaatg
    21001 tcggatgcga cgctggcgcg tcttatccga cctactccga attaatcttt cagcttgcgc
    21061 attaccagcg tggcgttggt gccgccgaag ccgaagctgt tagacataac tgtggtcagt
    21121 tcgcgatcgg tcgtttcggt cacgatgttc agacccgcag cctgctcgtc cagctcttca
    21181 atgttgatgc tcggggcgat aaaaccgtgt tccagcatca gcagagagta gatagcttcc
    21241 tgtacgccag cagcgcccag agagtgaccg gtcattgctt tggttgcaga aatcgccggg
    21301 ctcttatcgc cgaacacttc acggatagct gccagttctt tcacgtcgcc aaccggagtc
    21361 gaagtaccat gagagttcag gtagtcgatt ggggtatcaa caccgtgcat cgccatcttc
    21421 atgcagcgca ctgcaccttc gccagacgga gcaaccatgt ctgcgccatc agaggttgcg
    21481 ccgtagccaa cgatttcagc atagatgtga gcaccacgcg ccagcgcgtg ttccagctct
    21541 tcaaccacta ccataccgcc accgccagcg ataacgaaac cgtcacggtg agcgtcgtaa
    21601 gtacgggagg ctttttccgg ggtgtcgttg tatttagtag acagcgcacc catcgcgtcg
    21661 aattcgcaag ccatttccca gcacagctct tcgccgccgc cagcaaacac gatgtcctgt
    21721 ttgcccagtt ggatctgctc tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg
    21781 gagctgatgg agtagttaac gccgtgaatt ttaaacggcg tggcgaggca ggcagaaacg
    21841 ccggatgcca tcgctttggt gaccacatac gggccaaccg ctttcaggcc gcgcgggccg
    21901 cgcattgcgt cagcgccgaa aacctggaaa cgcggggagc cgccgccgga acctgcaatc
    21961 aggccaacgc gcgggttatt ctggtaagct tccggggaga ggccagcatc agcaattgcc
    22021 tgctccatag aaaggaatgc ataaatggat gcgtcgctca taaagcgcac aactttgcgg
    22081 tcaatgaggc cagtggtatc cagttttacg ttgccccaga cgtggctacg catgccggaa
    22141 tccttcagct cctgagagaa agtgatccct gaacgtcctt cacgcagaga tgccaggact
    22201 tcctgctggt tattaccgat gctggaaaca atgcccaggc cagtaatcac tgcacgtttc
    22261 attcaatacc tctgtaagtc gcacatagag taagtttcga atgcacaata gcgtacactt
    22321 gtacgccgaa caagtccgat cagccaatta atagagaaat ttgcgcagcc tgacacacat
    22381 cgctaagatc gagccaccgc ctgtaagacg agtaacttac gtgaaacact actccataca
    22441 acctgccaac ctcgaattta atgctgaggg tacacctgtt tcccgagatt ttgacgatgt
    22501 ctatttttcc aacgataacg gactggaaga gacgcgttat gtttttctcg ggggtaacca
    22561 tttagaggca cgttttcctg aacatccaca tcctctgttt gtggtagcag agagcggctt
    22621 cggcaccgga ttaaacttcc tgacgctatg gcaggcattt gatcagtttc gcgaagcata
    22681 tccgcaagcg caattacaac gcttacattt cattagtttt gagaaatttc ccctcgcccg
    22741 tgcggattta gcgctggcgc atcaacactg gccggagctg gccccgtggg cggaacagct
    22801 tcaggcgcaa tggcctttgc ccttgcccgg ttgccatcgt ttattgctcg atgaaggccg
    22861 cataacgctg gatttatggt ttggcgatat aaacgaactg accagccaac tggacgattc
    22921 gctaaatcaa aaagtagatg cctggtttct ggacggcttt gcgccagcga aaaacccgga
    22981 tatgtggacg caaaatctgt ttaacgccat ggcccggctg gcgcgtccgg gcggcacgct
    23041 ggcgaccttt acgtctgccg gttttgtccg ccgcggtttg caggaagccg gattcactat
    23101 gcaaaaacgt aagggctttg ggcgcaaacg ggaaatgctt tgtggggtga tggaactgac
    23161 attacccctc ccctgctcca cgccgtggtt taaccgtacg ggcagcagca aacgggaagc
    23221 ggcgattatc ggcggtggta ttgccagcgc gttgttgtcg ctggcgctat tgcgtcgcgg
    23281 ctggcaggta acactttatt gcgcggatga ggcccccgca ctgggtgctt ccggcaatcg
    23341 ccagggggcg ctgtatccgc ttttaagtaa acacgatgaa gcgctaaacc gttttttctc
    23401 taatgcgttt acttttgctc gtcggtttta cgatttattg cccgttaaat ttgatcatga
    23461 ctggtgcggc gtcacgcagt taggctggga tgagaaaagc cagcataaaa tcgcacagat
    23521 gttgtcgatg gatttaccca cagaactggc tgtagccgtt gaggcaaatg cggttgaaca
    23581 aattacgggc gttgcgacaa attgcagcgg tattacttat ccgcaaggtg gctggctgtg
    23641 tccagcagaa ctgacccgta atgtgctgga actggcgcaa cagcagggtt tgcagattca
    23701 ttatcaatat cagttacagg atttatcccg taaggatgac ggttggttgt tgaattttgc
    23761 aggagatcag caagcaacac acagtttagt ggtactggcg aacgggcatc aaatcagccg
    23821 tttcagccaa acgtcatctc tgccagtata ttcggtggcc ggtcaagtca gccatattcc
    23881 gacaacgccg gagctggcga aactgaagca agtgctgtgc tatgacggtt atctcacgcc
    23941 gcaaaatccg gcgaatcagc accattgtat tggtgccagt tatcatcgcg gcagcgaaga
    24001 gacggcgtac agtgatgaag atcagcagca gaatcgccag cggttgattg attgtttccc
    24061 gcaggtacag tgggcaaaag aggttgatgt cagtggtaaa gaggcgcgct gcggtgtgcg
    24121 ttgtgctact cgcgatcatc tgccaatggt aggcaacgtg cctgattatg acgctacact
    24181 cgtggaatat gcgtcgctgg cggagaagaa agatgaagcg gtaagcgcgc cggtttatga
    24241 cgatctcttt atgtttgctg ctttaggttc gcgcggtttg tgttctgccc cgctgtgtgc
    24301 cgagattctg gcggcacaga tgagtgaaga accgattccg atggatgcca gcacgctggc
    24361 ggcgttaaac ccgaatcggt tatgggtgcg gaagttgttg aagggtaaag cggttaaggc
    24421 ggggtaatct gctctggcat tgtttgtcgg atgcggcgta aacgccttat ccgacctacg
    24481 cgttacctgt aggcctgata agacgcgata agcgtcgcat caggcatggt gctccagacg
    24541 tcacaactta ctgctgtgag gcctgctgaa acaggtgttc ccacatatcg gtcaccagcg
    24601 cctggtcacg cggcgacaat tcaccggcac caatggcttt ttccagactc tggctaacgg
    24661 ttgtatgtac cgcctgagcg gagtggtcgt caccactttc cagttctgcg atggctaatg
    24721 tcaggtggcc acgcaaatac ccactggcaa acaactcatc atcactggca tggtcaacca
    24781 taccgtcgat taatgccaga atgcgtgatt caaactccgc gatcatcttc tttcctcatt
    24841 gtaaaacgtg gcgctggctt cagttgagcg cttccggcca cgggaactgt tccgccgtaa
    24901 gttccggcgt gtgataataa ttttgtagtg ctttaatgaa gcgtgccgga cgttcgggaa
    24961 tgccgtttgc cagataatcc atcacctgcg catgtacccg ccgttgaaac accacgcggt
    25021 ccggttcgaa atccccttcc agattgtcgc agctaacatt aaacggatat cccgctgcca
    25081 cgcagaacaa ccagtccagc gcctgcggct tcacttcaac atcttcaaac tggctttgtg
    25141 tctgggcatc gcgtccgtcc gggcaatacc agtagccgaa atcaaccagc tcacggcgcg
    25201 ctttcccggc aatacaccag tgcgaaatct cgtggatggc gctggcataa aagccatgag
    25261 cgaagacgat tctgttatac ggtacttccg catcagcagg aagatagatc ggttcgtcgt
    25321 cgcctttaat cagacgggta ttaaattcat cggcaaagca gctattaaaa atctcaatca
    25381 actgctcata gtggtgtgta ctgttcatta gttcatcccc aaccagtgga ggatctcctg
    25441 tccatggcta tcgtaaagta atttggcact catcaccgcc gagacgataa cgatcatcgg
    25501 gcggatcagc ttttgtcctt tgctcaacac cagtcttgaa cccatgcgcg cgccgaggaa
    25561 ctgccccacc agcatcacaa agcccgtcgc ccaaatcact ttgccgccga gaataaacag
    25621 cagcagaccg ccaatgtttg acgttgcgtt aagtaatttg gcgtgagcag tggctttggc
    25681 gaggttgaat ccgcacagcg taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc
    25741 aaaaaatcca tcgtaaaaac cgacgcagcc ccccgccacc agcgcaaatg gcagtccata
    25801 catccgacgc tggcgatctt cttcacccag ctttggcatc agcaagaaat agaggccgat
    25861 acaaatcacc agaataggca aaatctgccg taagacatcg gcctgaacat actgcaccag
    25921 tagcgcgccg ctcattgagc cgacaaaggt catggcgata ttgagttttt gatcgcttaa
    25981 actaaccact ttacggcgaa taaagtagat agtggcggaa atagagccac cgcaggcttg
    26041 cagtttattg gttgccagcg cgttagcggg agacatcccc gccgccatca atgccggaat
    26101 ggtgagtaac ccaccgccac cggcaatcga gtcgataaat cccgccagca tggcgacaaa
    26161 aaagagaact cccagcagca gcggggaaac cataaacagg ctattaaacg tttccattag
    26221 atcacgtgct catccagtag cgcctggcag gaaggcggca acggaggcgg tgtcttcttc
    26281 tcaggctttg ttgttcccgg ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa
    26341 ccatcgcctg gtggcggtaa aggttgatcc tcacactcca gactatcggc aggacaacgt
    26401 aatcgtacat gcatatgcgc gcgatgctgg aaccagggtc gcactttgcg caaccagtcg
    26461 cgatcggtgc ccgcatcaag gcaaagttgt tgcttaatcg ccggattaac aaaaatgcgc
    26521 gtgacgtctt tatcctgggc ggcgagtttg atcaagctga aaatttctgg cttccacagc
    26581 gtcgggacaa cgtgtttacc gtcgcgggaa actaaatcta acgcttgcgg gcgcaagagt
    26641 tgcgcggagg tccagcgtgt tttcggcagt tgcaggaaga tatcgacatc cagtccggtc
    26701 tggtggctgg cgtgaccgcc gttgaaacga ccaccagcag gcatccccat atcgccaatc
    26761 agcaccgtac ccatgcccag attgctcacc tggcgactca gacgctggat aaacatcacc
    26821 aggtctgggt gaccgaaata gcgacgctga tcggtacgca tgacctgata atgttcggac
    26881 tgtatcggca acgtgtcagc accgatgata cagccattag aaaaactgcc tatcgattgt
    26941 gcgctacccg gcacaggttg ggttattttt tgccacggcg tcgctgccag gctggcgcta
    27001 ctggcaagca gagccagcag cgcaatcgcg gttttgttca ttttttacca gcgtggaatt
    27061 tcagtcttca catcggcatt ttgcgcccgc tgccgtaaca ggtgatccat caaaacgatc
    27121 gccagcatcg cttctgcgat cggcaccgcg cggatcccaa cacaaggatc gtgacggcct
    27181 ttggtgatca tctcaacttc ttcgccaaag cggttaatag tgcgccccgg cacggtaatg
    27241 ctggaggtcg gtttcagcgc catgtgggca atgatttgct gcccgctgct gataccacca
    27301 agaatgccgc ccgcatggtt gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg
    27361 ctgccacgca gcgctaccac gtcaaaacca tcaccaattt ccacgccttt caccgcgtta
    27421 atgctcatta gcgcatgggc gatgtcggca tccaggcggt caaagaccgg ctcgccaagt
    27481 ccggcgggta cgccactggc aacgacggtg actttcgcac caatagagtc gccttctttt
    27541 ttcagcgcgc gcatcagttc atctaacgcg tcgattttgt ccgggtccgg gcagaagaat
    27601 ggattttgct cgacctgaga ccagtctttg atttccagcg gaatgtcgcc catctgggtc
    27661 aggcaaccgc gaatctcaat gccaaatttc tcggcgagat attttttggc aatcgcccct
    27721 gctgcaacgc gcatagcggt ttcacgggcg gaagaacgtc caccgccgcg ataatcacgc
    27781 aggccgtatt tttgttcgta agtgtaatcg gcatgacctg gacggaaaac gtccttaatc
    27841 gcactataat cctgagaacg ctggtcggta ttttcgatca acaatccgat gctggtgcca
    27901 gtagtaacac cttcaaaaac accggagaga attttgacct gatccggctc gcggcgctgg
    27961 gtggtatagc gcgatgtccc agggcgacga cgatcaaggt catgttgcag gtccgcttcc
    28021 gtcagcggaa tgcctggcgg aacaccatcg acgatgcagc cgagcgccag cccgtgcgat
    28081 tcgccgaagg tggttacgcg aaagagttgt ccaattgtgt ttccagccat cacggctccg
    28141 ttattgttgt gtttgcgtgt ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc
    28201 aataagctgc tctttagtga gcataaacac accatcgccg ccgttatcaa actccagcca
    28261 ggtgaacgga acatccggat attgttccat aagatgtacc atgctgttgc cgacttcaca
    28321 aatcagcacg ccatcatcag cgaggtaatc tgccgcgttt ccgagaatgc gacgcgtcag
    28381 tttcaggccg tcggtgccag atgccaggcc cagttccggc tcgtggcgat attcgtttgg
    28441 caggtcggac atatcttccg catcgacata cggcgggtta gtgacaatca ggtcgtactg
    28501 cactttcggc aagtcgcgga acaaatcgga ccgaatagga atgacgttgt ggatcagacc
    28561 gtgttcttcg atgttctgtt cagcaaccgc cagcgcgtcg ggagagatgt ccaccgcgtc
    28621 gacttccgct tccgggaagg cataagcaca ggcaatggcg atgcagccgc taccggtaca
    28681 catatctaaa atgtgctgcg gttgcttgct gatgagtccg gcaaatttat tgttgatcag
    28741 ttcaccaatc ggcgagcgcg gcaccagcac gcgttcatcg acgtaaaatt catggccgca
    28801 gaaccaggct ctgttggtca ggtaagccac cggaatgcgt tcgttgacgc ggcggatcac
    28861 gcgctcaaca atacggtgtt tttcgctgga ggtcagacgc gcggtgcgca tgtcttccgg
    28921 aatatccagc ggcaggtaga gtgaaggcaa caccagttgc acggcttcat cccacgggtt
    28981 atcggtgccg tggccgtacc agatattcgc cgcgctgaag cggctcaccg accagcgcaa
    29041 catgtcctga atggtttgca gctcatttac tgcttcatca acgaaaattt tatccacgta
    29101 ttcctccagg gcatgatcgc aataatttcg gcggctagtt tgccatgaag atgacgataa
    29161 atcagcattc acgcgcggtg agtgaggaaa aatacgttta aaacgatcga ttgcgctacg
    29221 agtcgggtaa actgtaggaa aattagaaat agagaaagat aaatgaaaaa gaaaacaaca
    29281 cttagcgagg aggaccaggc gctgtttcgc cagttgatgg cgggtactcg caagattaag
    29341 caggacacga ttgtccaccg accgcagcgt aaaaaaatca gcgaagtacc agtaaagcgg
    29401 ttgattcagg agcaggccga tgccagccac tatttttcgg acgagtttca accattatta
    29461 aatactgaag gtccggtgaa atatgttcgc ccggatgtca gccattttga ggcgaagaag
    29521 ttacgccgtg gcgattattc accggagttg tttcttgatt tgcacggtct gacgcagcta
    29581 caagccaaac aggaactggg ggcgttgatt gccgcctgcc gccgtgaaca tgtgttttgt
    29641 gcctgcgtga tgcatgggca cgggaagcat attttgaagc agcaaacgcc gctgtggctg
    29701 gcgcaacatc cacatgtgat ggcatttcac caggcaccaa aagagtatgg tggtgatgcg
    29761 gctttgttgg tgttgattga agtggaagag tggctgccgc cggaattgcc ctaataaata
    29821 ttgcggtata aacgtcttct ccgaccagga tactgaccgg agaagaatta accctacaac
    29881 tcattcaaaa tgtactggaa acgacaggtg gcatcgattg gctgctgact gctggtgtcg
    29941 atccgtattt gcatatcatg cagttgctta tcggtaattg gatgtgctac cggcgttact
    30001 gtgactttgc tgtcgtcaca ccccgacgtg ctgaatgtga tcatatacga cataaagcga
    30061 caataagcgc cacgatcgtc cattcggctt ccatcacaca cccgcccgtc gcgcatcagg
    30121 taagtcgaga gcgtttgtgt tatgactccc cccgtcgatt gcagcgttag catcttgtta
    30181 tggaggacgt agttctcatt ccaggagata tcataggggc caaccggctc cgttgggcac
    30241 cgggtttgtt ttgctgatgg accaatgtaa gtataacctc tcgcagcagt actcaccgtg
    30301 ttggtactga ccaaccacgg gcacccgtga gtttcaccat gcacgatgat atcgaccccc
    30361 agatcaacac atgaccatcc atccgtaatc cctgggcaat tggagttatc cacagtatta
    30421 ctctgatgag tgatgttcat cgttggtgct gatcccggtc ccgtccaaac cgtgttaggg
    30481 atattcccca accacaacgt atatccatcc tgtgcatatt cgccataata gaccccttcc
    30541 ggcgtcaaga catcgatacg aaagacaact tttgttgtag ccccgttacc ccaaaatccg
    30601 gtataagtct tcacatccct gaatgtgacg tccgcccagg cggttgcact cagtagcccg
    30661 gtcagcagta aaaaaaacag tccgctcatt tttttcatgt taaatatact caaacgtcgc
    30721 agtgagggag gccgtaaagt cacccatcgt cacatcccgc ccgctttttg cctgtagcca
    30781 ggcattaaaa tgcagatcat tgttgccgct gctcagcaca aatgtcgccc cgctggtatt
    30841 gttgattagc acagcagtgc cgtcggtctt ttccatgcca atccccacac cttgtgctgt
    30901 cgaagcggca tccagcgcca gaaaaccggg ctgctcgctg tcttccgttc cggtaagtgt
    30961 cacccgaacc tcatactgag caggcccttt gcaatcttta agctgaaaca ccaccggtac
    31021 gcgcgcagat tgcccggtca ccatcagatc tctacggctg accgtgggga aatgcacttc
    31081 cgccagcacc tcgccctgaa ccaccagcgt gcaggactta ctgagtaaat tgccgtagaa
    31141 atgcaggttg tctgccgcct gaacattcgg cgtcagcagc accgcggcca gcgcagccgc
    31201 gcagtgaaat atccgtttta ggctcattgg taatccacct gtaatgttgc ccaggcagaa
    31261 aaggttcctt cgctgagtga agcatcactg gctttcaccg gtacagcttg cagcgttggc
    31321 ggcgttgaca ggttgatttt cagcggcgta cccaacgtga acggctggcc attttgttgc
    31381 agctcaatcc ccagcccggt gacgtttgtc tcaatagccg cgttatcgta gctggtctgg
    31441 ctgcccgtcc agctcagcgt catctcccag gcatcgtcgc gcacgtcggg atcgcatgta
    31501 atggtatagg gcacattctg gcggaagtta ttgccgttga tgttgtcgat aatgacgtca
    31561 cgaaactcaa cttcaacggt tttcccgtca ctgatggcac aggcgggcgg cgaaaccaat
    31621 gtgccatgaa atgtgatatc gctatcggcc gcctgtgcaa taccgcaata acaaccgaat
    31681 aagcagagcg caaatcgttt cattgatact ccaccaccat tgtcatggtt gcgctaaact
    31741 ctgatgccgt aagctgtgtg ccgctttgtt tcaccggcac ggcttcgaaa cccgggcgcg
    31801 aattaacgtt aaacggcagc cagttattac ccgccaacgc aaccggcgca tggctggacg
    31861 catcctgtat gcgaatgcca aaaccattga tgcccgtact gaggaccgtt tcaccgttaa
    31921 tggtggtggt ggtcccctgt aactgcatct gtaggtcatc gctgacgctg ttcgaacagt
    31981 tcagggtgta accaacaggc tgccgatagt tgctgccgtc gatgttttta ataatgacat
    32041 tgccaaactc caccgcgctg ccttttaccg tacagggcgg cggtgcatcc accagtatac
    32101 gaagcgttaa gttgaccgtt ttactatcgg cccaggcgga gccgcaggcc agtaacacca
    32161 tgccagcgac gggtaataaa actcctttca tgctggatct cctgtcatta gccttttagg
    32221 ctttgagctt catccacctt gcaggtgtct ccttcacaac ggaagaccag cggtaaacgt
    32281 gcgccgtagt cgttgatgta ggtcagcacc ggagcgctgt tcatttttgc ctttaacggt
    32341 tgtgacgttt tcggcggcat caccagtggt ttaaagcccg cagcagttag cccctttttc
    32401 tgcgtactgg catcgctgat gatgacgtgg tacggcgtcg ggttattgac gatatagtcc
    32461 tggccgctgc gcgtgagtgt gaccttaaac tgccacggtt ttctcatgtc aatcttctcc
    32521 agcgcctttg gtcgccagaa cagcttgatg cgggtttgca gcgcaatttg cagggtgttg
    32581 gctttcccgg actttggcgg gatctcacgc acattgtagt agaacaggct ttcacggtca
    32641 gacggcagcg tgtgaatgtc cggcagcgcc tgaatcttca cctgaccgtt catcatggcg
    32701 tcaatacgct gcaccggcgg cagtaccgcc agcggcgaag tgattttgtt gcccttctcg
    32761 tcttccagcc agctttgcgc cagataaggc agcttttcgt tattgttgcg cagggttacg
    32821 cttatcgatt tatcactttc gttaaacacc agacgggtgc gatccggagt gactgatgcg
    32881 tgcgccgcca ggttggtggt ggtcaacagt accgccagcg tcgtgctgac caccattgaa
    32941 caggataatt tataaaacat tgattgttcc ttaatcatat tattgagtgg ctgaaattga
    33001 cgtcggcgct tcagatggcg tgacctgccg agtctggtcc tgaataacgg gcttaatttc
    33061 cggcgctaaa gaatcggggg ctgtcgttcc cttttgttca cacggcaaca acaggccgcg
    33121 gaacagatcg gcaggtagcg gcttcggcaa tacgatctcg cattgtgcgc tgccttccca
    33181 gaacaccatc atatgctcac ccgcattgat ccccgccaga tagacattgc cttcatcatc
    33241 cacaatgcca acctgctgct gctcgtcgtt ttttacttcc gcgccaaacg gcgggtagct
    33301 accgtcacgc aaccgcagta ccgccatcgc tttttgcccg ctgatcactt tgaatttacg
    33361 atagccgatt gcgccttccg tcagagtcgc ctgcaccacc gactgggtgg cttccgcatc
    33421 ttccggcaag ttgtttaaat ctatatacgc ctgattgcga tagtagttgt taatatcggc
    33481 gaccaccgcc ttgccaaaca tattggtgta caccggcgcg ccgttgcttt cgaccggcac
    33541 gttggcaata ccgtcggcgt caatcagcag gcgtgtgccg cccatgtttt gagtgcgatg
    33601 cagcgcccca ccgtgcgcgg tcagcgttgc cccgccctgt aaggcgatcc ccgccgaacg
    33661 gtattctcct tcatggtagt tggcgctgag atctaccttc gccaacgtgc cgtcgtggct
    33721 cagataacca tccacgctac cgtgctgttc gctggtgccg acgttaatct ggtagtgcgt
    33781 tgcgtcatcg atgcgattaa agtagccgac ctgactgctg tccgacccgc taccgtaaga
    33841 gccgttgtag gtcacggtgc tgctgtcgct ccacggaatg ctcatgctga ggtacattcc
    33901 cttatccgta ttatcgtcat attcgtagcg atagccggtc accgagatgc tcatattgcg
    33961 aatgctcccc atattaaaat agtgggaaaa catcaggtta tagtttgtct gttccgggcg
    34021 atcccagtaa gtacgatggg agtagttgag atagaccgag acacccgctg cggcaaagtt
    34081 ctggttatac gtgatcgtat acatctcttt gtcgtttccg gtccgcgcca tgtccgactg
    34141 gctcgcgtcc agatactcgc tcatagtcat aaagttcttt tcggagaaac ggtagcctgc
    34201 aaaggtgacg cggctgttaa gttcgtcaaa gtctttggca tagctcacgc gaaaggagtt
    34261 gccgtccagt tttcctttgc cgtatgcgct gtcatgatcc aggttgacgt gcgagtgagt
    34321 cacatcgaac gccagcgcgc caaactgtgc gaggtcgcgc cccaccccca tcgccgccga
    34381 ctggtagtgc tcatctgcca gcgcgccacc gtaaagcgac cagccatctg ccacccccca
    34441 tgatgcttct ccgccggaaa aaaatccgcc ttcggtcttg tggttccagt cttccggacg
    34501 ccccatcatc actttgtaac gcacctgccc ctgacgcgtg aggaatggca tggatgcggt
    34561 ggtgacgtca tattcctgca cctgaccatt ctgttcttca acacggacgt gtagtgtgcc
    34621 agagacagaa tcgccgatat cctgaatgcg gaatggcccg gcgggaacct gggtttcgta
    34681 aagtacccgg cccatctggc tgatggtcac ttttgcgctg ctgtgcgcca cgccagaaac
    34741 atctggcgca tagccgcgca gattgggcgg cagcatctgg tcatcggtgc tgacactgct
    34801 gccgatatag ttaaagccgt cgaaaatatc ggaattgaga taatcttctc ccagcgacag
    34861 cttcgctttt aaagagggta aagctcgcca ggcgtaataa cggctccagt cccagttttt
    34921 actcgttgta ctgttactgc tgtcatcttc atcgtcgttg ctgcgggtgt gctgataatc
    34981 actttgccag tctgcacgga aacgccatgc cccaaggttt gccccaactg taccgttacc
    35041 gctgatatca tgtgagtcct cgcccccttg ctcctgatgg cgagtttgcg cattgaggct
    35101 gtagtcggca atcagcccag aaataccatc gtcccagcgt gagggagggt cccagtcgct
    35161 gctggtatat tcgaggtaag cctgtggcac tgtcagcagc aaagccgact ggcttaaatc
    35221 attctcgact tccatcccgt ctaattgccc cggtttaagg cattcatcgt tgtgcgtcca
    35281 ctgcaggctt tttgctatcc cttctttcag ccccagcgcc gccactaact caggtgtcag
    35341 gcaggcataa gtttttgttg gatcgttttc agagacatac caattaatgt cgtattcatc
    35401 agaaagggat tgtttattta taaatacacg taaattatat ttacccgcgt cgacataccc
    35461 tttttttgaa aacttaccga gatcaatttt cgtgtcgccc tttaactcaa ggaaacgggg
    35521 gtcaaattga atggaatcat cagcccatgc attgactgat ccaccagaga ttgccagcgc
    35581 aatatagcag gcgattcccc gcagccgaaa atttgagtga ttaggcatac acatatccgt
    35641 ttgtgaaaca gtcgtaaatc cgttgtgcgt tgaataaccc cacagcagaa cgctgtgggg
    35701 ttgtcacatc agagataggt aatctggaat gtggtcaggg tttcgaactg gccgagatcc
    35761 ggcgcatcgg tttcacccac cagccaggct ttaaagttca gcgtttgctt gtctttaccc
    35821 ttgctagttg aagcatccat cacgattggc tgaacaatct tttcgcccac tttataacct
    35881 ttgccatggg tatcgccgat taccaggctc acgtggttca tggcaacacc gctgtcaaca
    35941 ttgaataacg catagttatc tgcgttggct ggccccgcgt agttagtgcc ggtgaacgtg
    36001 gtctccatgt ttgattcagt ggtaaacacg caatcctgta aatggatctg gaaatctttc
    36061 ggtgtggtag tgccgccgcc agtcagttta gccgcaccga tatcgcccat atcgacttcc
    36121 agtttatgat cgtctggaac gatggagcac ggggagtctt caatagtgcc gtaaaaacgt
    36181 gcggtaccgt tgttacctgc ggcaaaagca gaactggaga cgactgcgcc catgaccatt
    36241 gcggcagcaa tggctgtttt ggcaaactta ctcattattt gtccttaaat cattaaaagt
    36301 cataatataa gtacctgaca taaaattaat atatgttatg tccaatactt atagttagcc
    36361 attgaacata tacattccaa tataattata gaatgtaaac aagtattctc tggtgtgtct
    36421 ttatgttggt cttatctata aaaaaactca atttagtaat acttaaagac tttatgtaga
    36481 ttttattaat taatacatcg gcaaaatact ttcaccactt tccaacaaaa aaacatgcga
    36541 attatcaaca aaaaacaaac tatcataatc aacaaaaatg aattgcttat aaagcacact
    36601 aaaaaattat aatgacctta ccattattta ttgctaaaat ttaatattaa ttaaatcaat
    36661 aaatatatat tcaagacaca aaaccattat cttctttatt ttattacggg attcaataat
    36721 gggaaaataa ctatcgagag ttgttaaata ttaacaacat ttagacaaaa aaagaatgat
    36781 tgtatgacgg agaaactgcc cggtgtacgc gcacgtaacc gggcattgat tgcttaaatt
    36841 gcttttgcca tcttcaaatt acaagggctc atctgccagt tgaattggcc ttttccgctt
    36901 tcatcaagcg ttacgctggc aatcgctgac gtggtaaaca taggcggcgt ttcgcccgga
    36961 cataactcgg caaccagata cccaactaac ggtaagtggg agatcaccag taccgaagca
    37021 acaccttcat tggtcaacgc ctgcaaatag gcaccgacca gaccaacatc gccgcagggc
    37081 gttaactccg gcagaacttc tgcactggaa ggcaggttca gacaatcccc tacttcttca
    37141 agtgtttgct cggctcgcag gaacgggctc accagaacgc gttcgatttc cactttttga
    37201 cctttcagcc agttcgccat caggcgagat tcgtcacaac catgagtggt cagaggacga
    37261 accgaatcac tggcggcatc gagggctgcg tcgccgtgac gcatgataaa aacttgcata
    37321 ttgcaccgct tttgttaacc agattcgccc gttttctttc atctgaaacc agagaaagaa
    37381 aacggtggcc ggcattgtgc cttatccatt caccgaatga aacgctgttt tttacctcaa
    37441 tggcgtaagt atagtcaata tgcgtttaca ttttgagcaa cactccgcca ttcagcgcgg
    37501 attcatataa ctttgacctt cttattgcag gtcagttgca gttgttttcc aaaaactttc
    37561 cccacgctcg ctcatctcaa ctaaacgatc gcaaggggta aaacgggaac catactgcgt
    37621 ggccagtcgt tgcattattg caaccacttc gcctgcgccg agagaatcga tatagcggaa
    37681 cggtccaccg agaaatggcg gaaaaccaat gccaaatacc gcaccaatat ccccgtcacg
    37741 cacgctacgg ataacctgct cgtccagaca acgtactgct tcattcagca tcaacatcac
    37801 acaccgttca gcaacctgcg gtgcggagag tcgcccctgc ccttgtacgc caatgagcgg
    37861 gtaaatggcg ggatcgacct gttttttgct tttacgccct ttctgaccat aaagatagaa
    37921 accccgacca ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc
    37981 agacgcgcta aaacgttctc cataagcggc ttccagtaca ggcataattt tagtccctgt
    38041 gtcgattcct acctcatcca aaagttggat tgggcctacc ggaaaaccaa atttcactag
    38101 cgcggcatcg atatgctcaa tacgctcgcc ttcggtcaac atgcggatag cttcattgat
    38161 gtaaggcgct aagatgcgat tgacgtaaaa cccagctttg tcacgcacga caattggcat
    38221 tttgccctgt tttttcgcca gttttaccgt ggtggcgatg gtttgcgccg atgtactcgc
    38281 atgaggaata atctccacca gcggcatttt ttccaccgga ctgaagaaat gcaggccaat
    38341 aacttgctca ggtcgcgtgg cgtgagcggc gatatcgcca atcggtaaag atgacgtgtt
    38401 cgaagcaaag atggtatgag tagcgcaatt ttgctccact tccgccacca tctgttgttt
    38461 taattcgaga ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa agccgcaata
    38521 gtccgtcgtt ccggagatta atgccagctg tttgtcacgt tcgctggctt tgagatgacg
    38581 acggcgaact ttgccctcaa gctgatccca actgaacttc agcgcatgat ttatgccctg
    38641 cgggttgata tctttaattc tgaccggaag ccccgcttta caagcagtga cataagcaat
    38701 accgccgccc atcaagccac cacctagaat ccccacgctg tttaatggcg caggcggcgc
    38761 atcactgccg ggatcttttt tcacgtccgt actggcaaaa aagatattgc gcagcgcctg
    38821 cgattgcggc gtcatcgcca gttcaccaaa cgcccgagct tcggcgtcat agccactgct
    38881 ggtcccctgc gctaatcccg tttcgacaac ctccaggatg cgttctgtcg ccggatagtt
    38941 accttgcgtt ttgtgttctg ttttcttgcc gaccattttg aacagcagcg cacgacctaa
    39001 cggccccgcc agaatacgct cgcgaacagg tagagggcgt gaagatgggc gatcctgctt
    39061 tgccagctca acagcggctt ccagcaatat ggagtgcgga acgacgtcat ccaccaaccc
    39121 cagctttacc gcctgtttcg cccgaagttg ttttccggtg aggatcatct ctaatgctgt
    39181 gctgacacct atcaggcgcg gtaaacgctg ggtacctcct gaaccgggta acaatccaag
    39241 ttgtacttca ggcaaaccga gcaccgtttt aggatcgtca gtacaaacac gaccgtggca
    39301 cgccagcgcc aactccagcc cgccgcccag gcaagcgcca tgaatagccg cgataaccgg
    39361 aatgggcaaa gcatgaatct ccgccattaa ctgttgcccc tgccgcgcca gtacttccgc
    39421 ttcttgcgcc gttttgcagt tgccgatcat gttgatgtct gcgccagcaa tgaagttgtc
    39481 tggtttcgcg gagacaaaca ccacgccacg caactctttg ttttcacgga gttgcttaat
    39541 aatggcgcgt acctgcgagg caaattccgc cttcagggta ttcattttct cacccggtac
    39601 gtcgatggtg atgatagcaa tgttgtccag acgaacatta agagtaaacg ctgatgccat
    39661 ttccattatt ccgcctccag aaccattgct gcgccaagcc cacccgcagc acaggcggtc
    39721 actaaaccaa atccaccgcc acgacggcga agttcatgca atgtctgggt aatcatccgc
    39781 gcgccggtcg ctgcgaaggg atgcccgtaa gcaatcgaac cgccaagcac gttaaatttg
    39841 ctctcgtcca cttcgccagt ggcatgtgca cgccccagta cgtcgcgagc aaaacgttcg
    39901 ctacccagca actgaatatt ggccagcgtc tgggcggcaa aggcttcgtg catatcgatc
    39961 aatgtcagat cgcccatcgt caatccggca cgttccagcg ccagcggtgt tgaccaggct
    40021 ggaccgagca acatgtcctg ccagacatct atcgcagtaa atgcgtagct gcgcagatag
    40081 cccagcggta ccagccctaa ttctttcgcc cgggattcag tcatcaggat caccgctgcc
    40141 gcgccatcgg tcagcggcgt actgttcgcc gccgttaccg tcccgtgttt acgatcaaac
    40201 gccggacgca actttgcgta atcagcaaga gaggaattac cgcgaatatt gttgtcttcg
    40261 acaagcggtt gtttataagg agggataaag gcagtcatca cctcttcttt gagtttacct
    40321 tctgaccacg cctgagcggc acgctgatgc gaacggtgcg ccagtgcatc ttgctgttct
    40381 cgggtgatgc cgtaggtttt cgccatttgc tctgcggtgt cccccatccg caagccggta
    40441 gaatattctg ctaccgcagg cggtacgggc attaagtcgc gcaaacgcag gcgagagaag
    40501 agtttcaggc gctggctcat tgtgcgggct ttgttgacat caaccagtac gcgcgccagt
    40561 tttttactga cgccaattgg caatacggaa gaggaatctg ccccaccggc aatccccgct
    40621 cgaatagttc ccgccatcag gctttcggca acgttcgcaa ccgcctgaaa actggtggcg
    40681 caagcgcggc tgacgctgta agcatcagta tgcacattca ttccagtgcc aagaacaatt
    40741 tcacgggcaa tgttgggcgc ttccggcatt tgcacaacct gaccgaagac caattgttca
    40801 atcacttcag cggggatctc ggtgcgtgcc agcagttcgc ctaccaccat cttccctaaa
    40861 tcaaccgcgg gaatgccatg aaaagccgtc gcctgtcggg caaaaggcgt acgtaaaccg
    40921 ctaacaatgg cgatacgatc gccctggcgg gtaaccagcg gtaaaacctg acccataaca
    40981 ctcccctgta aaaaaataaa taaagtggtc tgacctgatc atagtcttaa ccattttttt
    41041 acatttagcc aagtggagaa aagggaaagt ggga
//
LOCUS       DAOFSV010000031        39413 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.031, whole genome shotgun sequence.
ACCESSION   DAOFSV010000031 DAOFSV010000000
VERSION     DAOFSV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.031"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(20..2545)
                     /gene="mrcB"
                     /locus_tag="QBH27_001722"
     CDS             complement(20..2545)
                     /gene="mrcB"
                     /locus_tag="QBH27_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDQ7014044.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQQPAQQEQKDSDGVAGWIKEMFGSN"
     gene            complement(2639..5068)
                     /gene="hrpB"
                     /locus_tag="QBH27_001723"
     CDS             complement(2639..5068)
                     /gene="hrpB"
                     /locus_tag="QBH27_001723"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDQ7014045.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSCLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFLRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPRLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSSSPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEASN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            5142..5672
                     /gene="thpR"
                     /locus_tag="QBH27_001724"
     CDS             5142..5672
                     /gene="thpR"
                     /locus_tag="QBH27_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDQ7014046.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5687..6391
                     /gene="sfsA"
                     /locus_tag="QBH27_001725"
     CDS             5687..6391
                     /gene="sfsA"
                     /locus_tag="QBH27_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDQ7014047.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            6569..7024
                     /gene="dksA"
                     /locus_tag="QBH27_001726"
     CDS             6569..7024
                     /gene="dksA"
                     /locus_tag="QBH27_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDQ7014048.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            7061..7987
                     /gene="gluQRS"
                     /locus_tag="QBH27_001727"
     CDS             7061..7987
                     /gene="gluQRS"
                     /locus_tag="QBH27_001727"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDQ7014049.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQNIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            8026..9444
                     /gene="pcnB"
                     /locus_tag="QBH27_001728"
     CDS             8026..9444
                     /gene="pcnB"
                     /locus_tag="QBH27_001728"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDQ7014050.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            9441..9920
                     /gene="folK"
                     /locus_tag="QBH27_001729"
     CDS             9441..9920
                     /gene="folK"
                     /locus_tag="QBH27_001729"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDQ7014051.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTILENLLRKNKFHELNKW"
     gene            10268..10852
                     /locus_tag="QBH27_001730"
     CDS             10268..10852
                     /locus_tag="QBH27_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014052.1"
                     /translation="MSKKLGFALSGLMLAMVAGSASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEISEGVLDTTVGAKAKPFSITVDCSKANPAPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVKINNPGDIYSKALDATSKS
                     ATYDFKASYVRADASLAATAGYVKTNTAYTVTYQ"
     gene            10949..11689
                     /locus_tag="QBH27_001731"
     CDS             10949..11689
                     /locus_tag="QBH27_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDQ7014053.1"
                     /translation="MFFNTKYTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVN
                     IRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     VLPKDRESVFWFNVLEVPPKPDAATVANQSLLQLAFRTRIKLFYRPEALKGNPSEAPL
                     ALKWSWSGSEGKAALRVANPTPYYVSFSSGDLEASGKRFPIDVKMIAPFSDEVMKVTG
                     LNGKANSAKVHFYAINDFGGAIEGNASL"
     gene            11724..14324
                     /gene="htrE"
                     /locus_tag="QBH27_001732"
     CDS             11724..14324
                     /gene="htrE"
                     /locus_tag="QBH27_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDQ7014054.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNGAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGVYDVSVYVNDQPVINQSITFVAIEGKKNAQACITLKNLL
                     QFHINSPDINSEKAVLLARDEALGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGGNYDFKNRYIQRDIASLRSQFIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     IEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDADDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDDQGNVIGNVGQGGQAFVRGIEQQGNINIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            14341..14913
                     /locus_tag="QBH27_001733"
     CDS             14341..14913
                     /locus_tag="QBH27_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014055.1"
                     /translation="MIKTTPHKIAILMGVLLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKITNKTTESQADFKLVASGCSSGISWIDTTLTGNAL
                     SSSSKLIIPLSGDLSSTTSNIGMGFKKRTTDDATFLQPNNSADKIRWSTEEMQPDKGL
                     EMTVALRETAAGQGVPGIFRALATFNFIYQ"
     gene            14928..15530
                     /gene="yadL"
                     /locus_tag="QBH27_001734"
     CDS             14928..15530
                     /gene="yadL"
                     /locus_tag="QBH27_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDQ7014056.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGRATQFSCVTPASQEVTISTA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            15557..16153
                     /locus_tag="QBH27_001735"
     CDS             15557..16153
                     /locus_tag="QBH27_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7014057.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVTTGAKNVGVVLFSIQDPANIFNVLSSAGNSRSIYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVMSHILVDVHYE"
     gene            16205..17458
                     /locus_tag="QBH27_001736"
     CDS             16205..17458
                     /locus_tag="QBH27_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDQ7014058.1"
                     /translation="MKTFFKYFVFLVLSCSCFTAISKDTSFLVGNNAGVGDGNFNGPS
                     TAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDGL
                     MDIENAMADSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYIGDTTP
                     QKFQWIITDPDLQHIGCDNAMRYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQDVSLSS
                     NSTYLYAFKAYSPGSRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKMG
                     EYAPGQIKNGATPVPFDISLQNCVRVGDIETKLSSGKLGTENKQLLGNTLTGSGAAKG
                     VAVLIEGLANRKSALMILKPNDSTSVYKDNTGQAQDHDSDAIYPEDQGITYPLHFQAT
                     LKQDGNIAIEPGEFKATSTFQVTYP"
     gene            17571..18365
                     /gene="panB"
                     /locus_tag="QBH27_001737"
     CDS             17571..18365
                     /gene="panB"
                     /locus_tag="QBH27_001737"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDQ7014059.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            18377..19228
                     /gene="panC"
                     /locus_tag="QBH27_001738"
     CDS             18377..19228
                     /gene="panC"
                     /locus_tag="QBH27_001738"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDQ7014060.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(19310..19507)
                     /locus_tag="QBH27_001739"
     CDS             complement(19310..19507)
                     /locus_tag="QBH27_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7014061.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLALRLLSKGM
                     SREDVAEMANLPLAEIDKMIN"
     gene            complement(19576..20496)
                     /locus_tag="QBH27_001740"
     CDS             complement(19576..20496)
                     /locus_tag="QBH27_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDQ7014062.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEVRQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            20770..21150
                     /gene="panD"
                     /locus_tag="QBH27_001741"
     CDS             20770..21150
                     /gene="panD"
                     /locus_tag="QBH27_001741"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDQ7014063.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(21154..22383)
                     /locus_tag="QBH27_001742"
     CDS             complement(21154..22383)
                     /locus_tag="QBH27_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDQ7014064.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGLGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTQVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(22447..22887)
                     /locus_tag="QBH27_001743"
     CDS             complement(22447..22887)
                     /locus_tag="QBH27_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ7014065.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(22992..23762)
                     /locus_tag="QBH27_001744"
     CDS             complement(22992..23762)
                     /locus_tag="QBH27_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7014066.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(23759..24685)
                     /locus_tag="QBH27_001745"
     CDS             complement(23759..24685)
                     /locus_tag="QBH27_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7014067.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            24794..25456
                     /gene="can"
                     /locus_tag="QBH27_001746"
     CDS             24794..25456
                     /gene="can"
                     /locus_tag="QBH27_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDQ7014068.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(25497..26033)
                     /gene="hpt"
                     /locus_tag="QBH27_001747"
     CDS             complement(25497..26033)
                     /gene="hpt"
                     /locus_tag="QBH27_001747"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDQ7014069.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            26239..28629
                     /gene="gcd"
                     /locus_tag="QBH27_001748"
     CDS             26239..28629
                     /gene="gcd"
                     /locus_tag="QBH27_001748"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDQ7014070.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLSVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(28676..30226)
                     /gene="cueO"
                     /locus_tag="QBH27_001749"
     CDS             complement(28676..30226)
                     /gene="cueO"
                     /locus_tag="QBH27_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDQ7014071.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRNLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            30392..30739
                     /locus_tag="QBH27_001750"
     CDS             30392..30739
                     /locus_tag="QBH27_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDQ7014072.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            30845..31711
                     /gene="speE"
                     /locus_tag="QBH27_001751"
     CDS             30845..31711
                     /gene="speE"
                     /locus_tag="QBH27_001751"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDQ7014073.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            31727..32521
                     /gene="speD"
                     /locus_tag="QBH27_001752"
     CDS             31727..32521
                     /gene="speD"
                     /locus_tag="QBH27_001752"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDQ7014074.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(32559..32921)
                     /locus_tag="QBH27_001753"
     CDS             complement(32559..32921)
                     /locus_tag="QBH27_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDQ7014075.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(33096..35693)
                     /gene="acnB"
                     /locus_tag="QBH27_001754"
     CDS             complement(33096..35693)
                     /gene="acnB"
                     /locus_tag="QBH27_001754"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDQ7014076.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            36048..37811
                     /locus_tag="QBH27_001755"
     CDS             36048..37811
                     /locus_tag="QBH27_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDQ7014077.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHDHHHHDDDDYHHHDGGHRDGNDWQ
                     HNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGM
                     SATQLPALSRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRE
                     FSRHQPLTQQQKDAARQRYQSASPEQRQAAREKMQTSPQNQQQRDAARQRIQSASPEQ
                     RQVFKEKVQQRPLNQEQRDNARQRVQSASPEQRPFFREKVQESRPQRLNDSNRTARLN
                     NEQRSAVRERLSERGARRLER"
     gene            complement(37882..39306)
                     /gene="lpdA"
                     /locus_tag="QBH27_001756"
     CDS             complement(37882..39306)
                     /gene="lpdA"
                     /locus_tag="QBH27_001756"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDQ7014078.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
BASE COUNT         9896 a        10393 c         9468 g         9656 t
ORIGIN      
        1 gcggagagcg attacgatat taattactac caaacatctc cttgatccag ccagccacgc
       61 cgtcgctgtc tttttgctcc tgttgagcag gttgctgttg ctgcggctga gaagactgat
      121 caaacggatt gcctgacggt tgctgctgca tctcactctg ctggcacagc gattgcggat
      181 cgctggtcca gactggcaag acacgcatac caccgctgca aacaaagttg ccgtcgtagt
      241 ccacgcccat atctgcaata tcttccggcg gaacaagatt cagcggtgtt ggcgtctggt
      301 tagccagata acgctgataa atcgacattg ccccgctggc accatacagt ttggtcggct
      361 ggttgttatc acggccgacc caggtgatgg tcaccgtgct cccatcaatg cccgcaaacc
      421 aggtatctac gttattgttg gtagtccctg ttttccctgc cagatgcagg ttcgggtatt
      481 ttgccccaag ctgacgtccc gtaccgcgtt gtacaacctg ctgcatggtc catagtgtca
      541 gatacgccgc ctgcgccgga acagcgcgtt ctgcctgcgg gaagctctga tacaacacct
      601 tgccgtcttc tgcgatgact gaacgtagcg cggaaagcgg agcacgatta ccaccgctgg
      661 cgatagtctg gaaggcctgc gccacttcga ttggcgttaa gttcaacgcc cccaacaaca
      721 ttgctggaac tggattcagt tgatctttcg gcacacccag tttgatccag gtatccgtaa
      781 ccgcaggcag ccccaacgcc atgcccagat tcaccgttgg cacgttcatc gaacgggtca
      841 acgcatctac cagcatcacc ctgccacttt cgctataacg acgatcatcg ttctgcggtg
      901 accagacctg gccattcggc tgacgcagcg cgattggcgc atcggcaatc cacgtattca
      961 aacgatagat tttcggctgg cttaaggccg ttagataagt cgctggtttt gcaagggaac
     1021 caatcgaacg acgcgcctgc atcgcacggt tgtagcccgc aaactgcggc tcagagccac
     1081 cgaccatcgc acgaacttca ccgctaaagc ggtcaacaac cacaatcgcg gtttcaagat
     1141 cgctcaactt acgctgtttc ttcagtgccg gaatgccttc cacggcggct ttttctgccg
     1201 cgtcctgggc caccgagtca aaggtagtga agatcttcac gccggagaga tcttttacct
     1261 tatcgcctaa tttcgcctgc agctcctgac gcaccagctg cataaaggct ggctgaggag
     1321 agatcacccc accgcgcggc tgaacaccca gcggacgtgc actcaacatg tcatagagtt
     1381 cttgatcaat aatttgttgc tgttgcagca aacgtagtac caggttacgt cgctccagcg
     1441 ccagtttcgg gttacgccac gggttgtaga tggacgcccc tttcaccata cccaccaaca
     1501 gcgcctgctg atcgaggctc aattcttcta ccgggcgacc aaagtaatac aagctcgcca
     1561 gcgggaagcc gcggatttcg ttgtcgccgc tctgaccgag atacacctcg ttcatataca
     1621 gctcaagaat acggtctttg ctgtaacgcg cgtccatgat cagcgccatg taggcttcgt
     1681 tcgctttacg ccagtaagaa cgctcgctgg agagaaacag gtttttaacc aactgttgcg
     1741 tcagcgtact cgccccctgc accgtgcgtc cggcagtcag gtttgccagc accgcacgtc
     1801 cgattgagta gagactgatc ccatcatgct cgtagaaatg acggtcttct gtcgccagca
     1861 aagtatccac cagcaaatcc gggaaaccac tgcgcggcac aaacagacgc tgctcaccgt
     1921 ttggcgaaga gatcatggtg atcaggcgcg gatcaagacg gaagaaaccg aactgacggt
     1981 tgttctccat attgactatc gtcgccagat gatcgccatc aaaggtcaga cgcgcgcgca
     2041 cctgcccttc tttgctgtcc gggaaatcaa acggacggcg gatcatctca atgctgttgg
     2101 cctgtacggt aaactcacca ggacgggtca ttttcgacac ctggcgatac tgggtcgcct
     2161 ccagcagctt caccatctcg ttcttgctga tggtcatgtc tggctcaagg ttgaccattc
     2221 gaccataaac tgccgcgggc aattgccaga ccttgccatc aatacggctg cgaatttttt
     2281 gatcgagata aacgccgtaa atggcgatca gcacggcaaa aacgatagcc agttttagca
     2341 gcagccatag ccagccgcgt ttgccacgag gcttacgccc tttgcctttg cccttacctt
     2401 tgcgcggcat cggttcttca tcctcatagt catcataatc gtcgtaatca tcgtcatctt
     2461 cgtaacgacg acggcttacc ttttgtttga ccggacgcgt cggtttccct ttgcgtccaa
     2521 ttggctcgcg gtcattcccg gccatgcttt ttctccgcaa tattcaggcg caaaagcccg
     2581 attttctgtt cttctgttgc aagacagaag aagatatctc tcaaatttgc tttggtcttc
     2641 acgaatactt tttcgttcgt cgcgtcggtg cggtgtttgc cgggtcgtcc ggccagacat
     2701 gtttgggata acgccctttc atctcttttt gcacctcacg gtacgctcct ttccagaagg
     2761 cgctcaaatc acgcgtgatt tgcagcggcc tttgggcagg ggaaagcaac tccagcacca
     2821 acggtacgcg cccctgggcg atagtcggat tagaggcctc accaaacatc tcctgcattc
     2881 tcaccgccag cgcgggcggg ttatcttcat ggtaacgaat ggcgatccgg cttcccgtcg
     2941 gcacagtgta atgcgcaggc aattcactat ccagacgttg ctgcattacc caatcaagta
     3001 atccacgcag tgcctgataa atatcgagtg atttcaggcc gcgtagcgaa tgtacgcctg
     3061 tcatatgcgg cagtagccac gtttccagcg tcgccaacaa actttcatca tcaaccgctg
     3121 gccagtcata ttccggcaac cactttgcgg cacataacaa acgcaagcgt agctgttccg
     3181 cttccgccgt ccagttgagc acgcttaaac ctttatcacg aatgccgtta agcatcgcct
     3241 gatgcaactc gtcttccgag ggtttcgcca gcggttgcac ttttaccgtc aactgaccga
     3301 tttgcagccg acgccaggct ttcagcgtac cttgcgcgtc atcccactcc acggtgtcag
     3361 actgctgtac cagctgcggg cagcgttgta ctaactcatc aatatcgacc ggcagcgcca
     3421 gtaaaatccg cgcatccggc gaagaactgc cctgcaataa taacggcgcg atcaaccatt
     3481 cgtggcggct aagcgcatcg tcggcatcga gcatcgcccc catgccgttc gccagttgat
     3541 agcgtccctc ttgcccacgg cggcgagcaa tacgatcggc atacgctccg gcaagtcgcg
     3601 gcgcgataag cgaactgtct gcctcaccgc cgcgtacgtt taaacgtttt aacagttgct
     3661 gactacgttg ctgccaggcg ggttgattgc gcaaaaacgc cacgcccagg tcactattgc
     3721 ccatccgtgg cggctcttcg agaatggcgg caatttttgc cgcggtagca gcttcgtcgt
     3781 cgttcttcgc gctaaccagc attgccgcta aacgcggatc gttacccagc gctgccattt
     3841 tttgcccttg cgcactaagc cgttcaccgt ccagcgcccc cagcatccgt aacaggcgtt
     3901 tcgcggccag tagattcacc gttggcggtt gatccagcca gctcatctgc gccggatcgc
     3961 tgcatcccca ttgcagtaat tccatcagca aacaggaaag atcgctttgt aatatttccg
     4021 gttcactttg cgcggcagcg cgttctgctt gttctttggc gattaaatgc aggcagatac
     4081 ccggctccag acgcccggca cgcccggcac gttgcgtcat cgacgcctga ctaacacgtt
     4141 gagtaaccag ccgcgtaagc cctgtacgcg gatcaaaacg cgccacacgc tcctgggcac
     4201 aatccaccac cagacgaata ccttcgatgg ttaaactggt ttcagcaata ttggtcgcca
     4261 gcaccacttt acgcatccct tgcggtgccg ggaggatcgc ttttcgctga tcgttcagcg
     4321 acaacgcgcc atacagcggg cagagcaaca catcactgcc gatgcgtgaa gtcagttgtt
     4381 cctgcacacg ctgaatttct ccgacgccag gtaaaaataa cagtaatgat ccactttcct
     4441 gacgcagcat ttcggcggtg gcaaccgcga cggcttcgtc aaaacgctga tgagcgggca
     4501 gcggcaaata acggcgttca actggaaacg agcgcccttc tgagatgacg acgggcgctt
     4561 ctggcagcat ttgctgcaag cggtcgttgt ccagcgtagc cgacataatc agcagtttaa
     4621 ggtcatcacg cagaccttgt tgcacatcga gtaacagcgc caacgccaaa tccgcctgca
     4681 agctgcgctc atgaaattca tcaaggatca ccaatccaac accgctcagt tccgggtcac
     4741 gctggatcat ccgcgtcagc acgccttcgg taaccacttc caggcgggta ttcggcccga
     4801 cgcagttttg cgcacgcatc cggtagccaa cggtatcgcc tggcttttcg ttaagcagct
     4861 ccgccagtcg ttgcgcaacg ttacgcgccg ccagacgacg cggctccagc aggataattt
     4921 tcccgttaat gccgggatgc gccagcagtt gcagcggcag ccaggttgat ttcccggccc
     4981 cggtcggcgc acttaataac acctgcgatg caccatcgag ggcagcaagt aactcaggta
     5041 agacggcagc aacgggcaac gacgacacaa aacgctccag agggttaaca ttcttcgcgc
     5101 cacattgtag catcgcggta attcataacc gagtgcctca catgtctgaa ccgcaacggc
     5161 tgttctttgc tatcgactta cctgcagaaa tccgcgaaca gattatccac tggcgcgcca
     5221 cacacttccc acctgaggcg ggacgtccgg tcgccgccga taatttgcat ctgactctgg
     5281 catttttagg cgaagtgagc gcagagaaag agaaggcgct ttctctttta gccggacgga
     5341 ttcgtcaacc tggtttcaca ctcacgcttg atgacgccgg tcaatggctg cgttcgcgtg
     5401 tggtgtggtt agggatgcga cagccgccac gcggcttaat ccagctggcg aatatgctcc
     5461 gttcacaggc tgcccgcagc ggttgttttc aaagcaatcg tccgtttcat ccacatatta
     5521 ccttattgcg cgacgccagc gaggcggtga caatcccgcc gccaggtttt aactggtcgt
     5581 atacggtgac ggagttcacc ctttacgcct cctcgtttgc ccgtggacgc acacgctaca
     5641 cgccgctaaa acgctgggcg ctaacgcaat aacaaggatt gtcgcaatgg aattttctcc
     5701 ccctctacag cgcgcgacgc taattcagcg ttacaaacgt tttttagccg atgtgatcac
     5761 acccgatggt cgcgaattaa cgctacactg cccgaatacg ggtgcgatga ccggttgtgc
     5821 aacgcctggc gataccgtct ggtattcgac ttcagacaac accaaacgga aatacccaca
     5881 cacctgggaa ttaactcaaa gccaaagcgg cgcaattatt tgcgtcaata cgctttgggc
     5941 taacaggttg acgaaagagg ctatccttaa tgaatcaatt tcagagctgt caggctatag
     6001 ctcgctgaaa agcgaagtaa aatacggcgc cgaacgcagc cgtattgact ttatgttgca
     6061 ggcggattcg cgtccagact gctatattga agtgaaatcg gttacgttag cggagaacga
     6121 acagggatat tttcccgatg cggtcactga acgaggtcag aaacaccttc gggagttgat
     6181 gagcgtagcg gctgaaggcc agcgtgcggt tatctttttc gccgtgctgc attcagccat
     6241 tacacggttt tcacccgcgc gccacatcga tgagaaatac gcacaactat tgtcagaagc
     6301 tcaacagagg ggggtagaaa ttctggctta caaagcggaa ctttctgccg aaggcatggc
     6361 tcttaaaaaa tcactaccgg ttacattgta gtagagtaag taactggtta atttacattc
     6421 tggtcgcgtg cgcaaatacg cttttcctca cacagttgtc aagtgttacg tttagataat
     6481 tgctatccgg aaaagcatct gctatttata gcggcctcat ttttcccccg aacatgggga
     6541 tcgatagtgc gtgttaagga gaagcaacat gcaagaaggg caaaaccgta aaacatcgtc
     6601 cctgagtatt ctcgccatcg ctggggtgga accatatcag gagaagccgg gcgaagagta
     6661 tatgaatgaa gcccagctgg cgcacttccg tcgtattctg gaagcatggc gtaatcaact
     6721 cagggatgaa gtcgatcgca ccgttacaca tatgcaggat gaagcagcca acttcccgga
     6781 cccggtagac cgtgcagccc aggaagaaga gttcagcctc gaactgcgta accgcgatcg
     6841 cgagcgtaag ctgatcaaaa agatcgagaa gacgctgaaa aaagtggaag acgaagattt
     6901 cggctactgc gaatcctgcg gtgttgaaat tggtattcgc cgtctggaag cgcgcccgac
     6961 agccgatcta tgcatcgact gcaagacgct ggctgaaatt cgcgaaaaac agatggctgg
     7021 ctaattacag ccgttccatc atgtttacca caggcgggaa atgctcccgc cttatttttt
     7081 gttcaaagag atgacagaca cacactatat tggtcgcttc gccccctctc cttccggcga
     7141 gcttcatttt ggttctctga ttgctgcgct tggcagctat ttgcaggctc gcgcccggca
     7201 aggtcgctgg ctggtacgca ttgaagatat cgacccgcct cgtgaagttc ccggtgccgc
     7261 agagactatc ctgcgccagc tggaacatta cggcctacac tgggacggcg acgttctctg
     7321 gcaatcgcaa cgtcaccacg cctatcgcga agcactcgcc tggttacatg agcaaggact
     7381 aagttattac tgcacctgca cgcgtgcgcg tattcaaaac attggcggta tttacgacgg
     7441 tcattgccgg gatttgcatc atggaccaga caacgccgca gtacgtatcc gccagcagca
     7501 tccagtcacc cagtttactg acctgctgcg aggcattatt cacgccgacg aaaaactggc
     7561 acgggaagat tttattattc atcgccgtga tggtttgttt gcctataacc tggcggtggt
     7621 ggtggatgat cacttccagg gcgtgagcga aattgtccgc ggggcagatt tgatcgaacc
     7681 gactgtgcgg caaatctcgc tgtaccagct ttttggctgg aaagtgccag attacatcca
     7741 tttaccgctg gcgcttaatc cacaaggcgc taaactttcc aagcagaatc atgcgcctgc
     7801 gttgccgaaa ggcgatccgc gcccggtgtt aatcgcggca cttcattttc tgggtcagca
     7861 ggtagaaaca cactggcagg atttcagcgt cgagcaaatc cttcagtcag ccgtcaaaaa
     7921 ctggcggctc accgcagtgc ctgagtcggc aattgtaaat tcaacattct caaatgcgtc
     7981 atgctgagct atgattagcc gctatttttt tgtcctgaat gatgtttgac actaccgagg
     8041 tgtactattt ttacccgagt cgctaatttt tgccgcaagg tgctaagccg cgaggaaagc
     8101 gaggctgaac aggcagtcgc ccgtccacag gtgacggtga tcccgcgtga gcagcatgct
     8161 atttcccgca aagatatcag tgaaaatgcc ctgaaggtaa tgtacaggct caataaagcg
     8221 ggatacgaag cctggctggt tggcggcggc gtgcgcgacc tgttacttgg caaaaagccg
     8281 aaagattttg acgtaaccac taacgccacg cctgaacagg tacgtaaact gttccgtaac
     8341 tgccgtctgg tgggtcgccg tttccgtctg gctcatgtga tgtttggccc ggagattatc
     8401 gaagttgcga ccttccgtgg acatcacgaa ggtaacgtca gcgaccgcac gacctcccaa
     8461 cgcgggcaaa acggtatgtt gctgcgcgac aacattttcg gctccatcga agaagacgcc
     8521 cagcgccgcg atttcactat caacagcctg tattacagcg tggcagattt taccgtccgt
     8581 gattacgttg gcggcatgaa ggatctgaaa gacggcgtta tccgtctgat tggtaacccg
     8641 gaaacgcgct accgtgaaga tccggtacgt atgctccgcg cggttcgttt tgccgccaaa
     8701 ttgggcatgc gcatcagccc ggaaaccgcc gagccgatcc cacgcctggc aaccttactg
     8761 aacgatatcc caccggcacg tctgtttgaa gaatcgctga aactgttgca agcgggctac
     8821 ggttacgaaa cctataagct gctgtgtgaa tatcatctgt tccagccgct gttcccgacc
     8881 attacccgct acttcacgga aaatggcgac agcccgatgg aacgcattat tgagcaggta
     8941 ctgaagaata ccgatacgcg tatccataac gatatgcgcg tgaacccggc gttcctgttt
     9001 gccgccatgt tctggtaccc attgctggag acggcacaga aaattgctca ggagagcggc
     9061 ctgacctatc acgacgcttt cgcactggcg atgaacgacg tgctggacga agcctgccgt
     9121 tcactggcga tcccgaaacg tctgactacg ctaactcgcg atatctggca gttgcagttg
     9181 cgtatgtccc gtcgtcaggg taaacgcgca tggaaactgc tggagcatcc taagttccgt
     9241 gcggcatacg acctgttggc cttacgagcc gaagttgagc gtaacgctga actgcaacgt
     9301 ctggtgaaat ggtggggtga gttccaggtt tccgcgccgc cagaccaaaa agggatgctt
     9361 aacgaactgg atgaagaacc gtcaccgcgt cgtcgtactc gtcgtccacg caaacgcgca
     9421 ccgcgtcgtg agggtactac atgacagtgg cgtatattgc cataggcagc aatctggcct
     9481 ctccgctgga gcaggtcaat gctgccctga aagcattagg cgatattcca gaaagccgca
     9541 ttcttgccgt ttcttcgttt taccgcaccc cacctctggg gccgcaagat caacccgatt
     9601 acttaaatgc cgccgtcgcg ctgaaaacca ctcttgcccc tgaagagcta ctcaatcaca
     9661 cacagcgtat cgaattgcag caaggtcgcg tccgtaaagc tgaacgctgg ggaccgcgca
     9721 cgctggatct cgacatcatg ctgtttggta atgaagtaat aaataccgaa cgtctgaccg
     9781 ttccgcacta cgatatgaaa aatcgtggct ttatgctgtg gccactgttt gaaattgcgc
     9841 cggagctggt ctttcctgat ggaacgatat tagaaaactt attaagaaaa aataaatttc
     9901 atgaattaaa taaatggtaa tgtaacagta acgtgaatat aaataaattc cgaaaaataa
     9961 cctcacccta ttgaaattaa tacaccctct cagttacaat cataattaat attatttttt
    10021 cgccccccca ccccatctca catacaatac ttttcccgct gtgtatatta acttttgctt
    10081 aaaaatgaat taagtggata tttaaattta aatgtgtaca ttattctact tagtgatgaa
    10141 cagatgttaa attcagtaaa tcttcacatt catcattaac aaactaatct cttttcctct
    10201 atcgggaatc cctgcctgaa agggcccaat tgtagttaaa ctcatggaat atgattcaag
    10261 gatttttatg tctaaaaaat taggttttgc gctctctggt cttatgctgg caatggttgc
    10321 aggtagtgct tctgcagata tggacggtgg tcagttaaat atcagtggtc tggttgttga
    10381 taatacctgt gaaacccgcg tagacggcgg taataaagat ggcctgatcc tgctgcaaac
    10441 tgcaaccgtt ggagaaatct ctgagggtgt gctggacact accgttggtg ctaaggctaa
    10501 accattcagc attactgttg actgtagtaa agctaatccg gcaccaggca gcacagcaaa
    10561 aatgactttt ggttcagtct tctttggtaa cagtaaagga accctgaata atgacatgtc
    10621 aattaatact ccatctgacg gtgttaatat tgctctgcac aatattgaag gttctactat
    10681 taagcaggtt aaaatcaaca atcctggcga tatctattcc aaagcactgg atgcaacttc
    10741 caaatccgca acttatgatt ttaaagcgtc ttatgttcgt gcagatgcaa gtctggcagc
    10801 cacagcaggt tatgtaaaaa ctaacactgc atataccgtt acttatcagt aatcacttcc
    10861 attcatattc ataaattgaa tacatacagg gaataatatt gatactccat actatttcct
    10921 gtttttttaa ctctaccagg aatggtttat gtttttcaac actaaatata cagcagcatt
    10981 atgcttcgca acctgtgtag cttttagctc gtcagctata gcagatatcg taatttccgg
    11041 tacacgcgta atctataaaa gcgaccaaaa aagtgtcaac attcgcctgg aaaataaagg
    11101 caataaccca ttacttgttc agagttggct ggatactggt gatgacaatg ctgagcctgg
    11161 cagtattacc gtccccttta ctgcgactcc gcccgtatca cgcattgatg caaaacgcgg
    11221 ccaaaccatc aaactgatgt acaccgccag cagcgtcctg ccaaaagaca gagaaagtgt
    11281 gttctggttt aacgtactag aagttcctcc aaaaccagat gccgcaacag ttgcaaatca
    11341 aagcctgctg caattggcat ttcgcacacg tataaaactg ttctatcgcc cggaagcatt
    11401 gaaggggaat ccctcagaag caccattagc cctgaaatgg tcctggtcag gttccgaggg
    11461 taaagcagca ttacgagtgg ccaatccaac cccttactac gtctctttta gcagtggtga
    11521 tttagaagcc agcggtaaac gctttccgat tgatgtgaaa atgattgcac cattcagtga
    11581 tgaagtcatg aaagtcactg gccttaatgg caaagcaaat tctgcaaaag tgcattttta
    11641 cgccattaat gattttggtg gtgccattga aggtaatgcc agtctgtaat caggcaagga
    11701 tataattccg caggaagcat aacgtgacta taaaaccaac taaaaattat cacaatcatc
    11761 tgacccgtat cgccacgttt tgcgcgctgc tgtattgcaa tggtgctttc tgtgctgaac
    11821 ttgttgaata tgaccatacc ttcctgatgg ggcagaatgc ctcaaatatt gatctcagcc
    11881 ggtatagtga aggtaacccc gctataccgg gtgtgtacga cgttagtgtt tatgtcaacg
    11941 atcaacctgt tattaaccaa agcattacat ttgtcgcaat tgaaggaaaa aagaacgcac
    12001 aagcctgtat cacacttaaa aatttattgc agttccatat taattctccc gatataaaca
    12061 gcgaaaaagc cgttctgctt gcccgggatg aagctctcgg caattgcctc aatttgacgg
    12121 aaattatccc tcaggcttcc gttcgttatg acgttaacga tcaacgtctg gatatagacg
    12181 ttcctcaggc ctgggtaatg aaaaattacc aaaactatgt tgatccatcg ttatgggaaa
    12241 atggcattaa tgcagccatg ttgtcataca acctcaacgg atatcatagt gaaacccctg
    12301 gtcgaaaaaa tgacagcatt tatgctgctt ttaacggtgg gatgaatttg ggtgcatggc
    12361 ggctgcgtgc ctcaggcaac tacaactgga tgaccgattc tggcggtaat tatgatttta
    12421 agaatcggta tatacagcgt gatatcgcct cactacgctc tcaattcatt attggtgagt
    12481 cttatacgac gggcgaaacc tttgattccg tcagtatccg aggcattcgc ttatacagtg
    12541 acagccgcat gttgcctccg acattagcca gctttgcgcc tatcattcat ggcgttgcca
    12601 ataccaacgc caaagtcact attacgcaag gtggctataa gatttatgaa acgacggtgc
    12661 cgccaggcgc tttcgtcatt gatgatctga gtccatcagg gtacggcagt gatcttattg
    12721 ttaccatcga agaatccgat ggctcaaagc ggacattctc gcaacctttt tcatcggtcg
    12781 ttcaaatgtt acgccctggc gttggacgtt gggatattag cggcggccag gtcttaaaag
    12841 atgatattca ggatgagcct aatttatttc aggccagcta ctactacggt ctgaataact
    12901 atctgacggg ttataccggt attcagatta ccgataataa ctataccgct ggtttgttag
    12961 gtcttggtct gaatacttcg gtcggtgcat tttctttcga tgtgactcac tccaatgttc
    13021 gtataccgga tgataaaaca taccaggggc aaagttatcg tgtttcctgg aacaagttat
    13081 tcgaagaaac aagtacttca ctgaatatcg cggcctatcg ctattcgaca cagaattatc
    13141 ttggtcttaa tgatgcacta actctgattg atgaagtgaa acatccagaa caagatcttg
    13201 aaccgaaatc catgcgtaat tactcgcgca tgaaaaatca ggttacagtc agtattaacc
    13261 aaccgttgaa atttgaaaaa aaagattacg gttcttttta tctttctggt agttggtccg
    13321 attactgggc ttccggacaa aatcgtagca attactccat tggctacagt aacagtgcat
    13381 cctggggcag ctacagcatc agtgcacagc gttcatggaa tgaggacggc gacgctgatg
    13441 atagcgttta tctcagtttc accattccta ttgaaaaatt acttggcact gaacaacgta
    13501 cttcaggttt ccagagtatc gatactcaaa tgagcagtga ctttaagggt aataaccaac
    13561 tcaacgtcag tagcagtggc tatagcgata acgctcgcgt cagttatagc gtgaatactg
    13621 gctatacgat gaataaagcc agcaaagatt tgagttatgt tgggggttat gccagctatg
    13681 aatcaccgtg gggaactctg gcaggttcag tttctgcaaa cagcgataac agtcgtcaag
    13741 tttctctcag caccgacggt ggttttgtat tgcatagtgg cggcctgacc ttcagtaatg
    13801 acagttttag cgactccgat acactggcgg tagttcaggc tccaggtgct caaggagcgc
    13861 gaataaatta tggcaacagt actatcgatc gatggggtta tggtgtcacc agcgctcttt
    13921 ctccttatca tgaaaaccgt atcgcgctgg atatcaacga tcttgagaac gatgttgaat
    13981 taaaaagtac cagtgctgta gctgtgcctc gtcagggttc tgtcgttttt gctgattttg
    14041 aaaccgtgca agggcaatca gccattatga acatcacacg tagtgatggc aaaaatattc
    14101 catttgctgc agatatttat gatgaccaag gcaatgtcat tggtaatgtt ggacagggtg
    14161 gacaggcttt tgttcgtggt attgaacagc aaggaaatat caacattaaa tggctcgaag
    14221 aaagtaaacc tgtaagttgt cttgcgcatt atcaacaaag ttcagaagca gaaaaaatag
    14281 cgcaatctat tattctgaat ggaattcggt gtcagattca gtaactacaa gggacgtcaa
    14341 atgataaaaa caacgccaca taaaatagcg atactgatgg gagtattatt atcaccctca
    14401 gtatttgcaa cggatattaa tgttgagttt acagccactg tcaaagcgac aacctgtaac
    14461 atcacactta ctggtaataa tgtcacgaat gatggcaata ataactacac tttgagaatc
    14521 cctaagatgg gcctggataa gatcacgaat aaaacgacag aatctcaggc tgattttaaa
    14581 ctggttgcca gtgggtgcag cagtggcatt agctggattg ataccactct gaccggaaac
    14641 gcattatcaa gctcatccaa gcttattata ccgctgtctg gtgatttatc ttcgacgaca
    14701 agtaatatcg gtatgggttt caaaaaacgg actactgatg atgccacttt ccttcaacct
    14761 aacaacagtg cggacaagat acgctggagc acagaagaaa tgcagcccga taaaggtctt
    14821 gaaatgactg tcgcgctacg tgaaaccgca gcagggcaag gcgtgccggg gatttttcgc
    14881 gccctggcga cgtttaattt catctatcaa taagaaggat aattaagatg acatttaaaa
    14941 atttacgtta tggattatcc agcagtgtcg ttttggctgc ctcattgttc agcgtaccct
    15001 cttatgcggc aactgacagc attgggctga ccgtgattac caccgtagaa atgggtactt
    15061 gtaccgctac attagtaaat gactctgatc aggacatttc tgttgttgat tttggtgatg
    15121 tatatatttc tgaaatcaat gccaagacca aagtaaaaac attcaaactc aaattcaaag
    15181 actgtgcggg tatccccaat aaaaaagcgc aaataaaatt aaccaagcga gccacatgcg
    15241 aggggactgc taatgacggt gcagggttcg caaatggttc cacagccaca gataaagcaa
    15301 gtgccgtcgc cgttgaagtc tggagcaccg aaactccggc aacagggaga gcaacacaat
    15361 ttagttgtgt gacaccagca tctcaagagg taactatttc cactgctgcc aatgcggttg
    15421 tttattaccc gatgagtgct cgcctggtcg ttgaaaaaaa taaaaccgta agtaatgtca
    15481 ctgcaggtaa attttctgca cctgccacat ttacagtaac ctataactaa cagactgaca
    15541 gattcaggaa ctatatatgc atcccactca acgtaagctg atgaagagaa taattctgtt
    15601 tctgtcatta ctgtttccca ttacctgtcc agcgatcgct ggacaggata ttgaccttgt
    15661 tgctaatgta aaaaacagca cttgcaaaag cggaatcagt aaccagggta atattgatct
    15721 tggtgtcgtt ggggtgggat atttttcagg taatgttact cctgaaagct atcaaccagg
    15781 tggaaaagag ttcactgtca ctgtctcaga ctgtgcatta cagggaactg gcgatgtgct
    15841 aaatcagtta catattgatt ttagagccct tagcggtgtc atggctgcag gctccaggca
    15901 aatatttgcc aatgaagtga caaccggggc taaaaatgtc ggtgtcgttc tcttttccat
    15961 tcaggacccg gccaatatat tcaatgttct tagttcagcg ggaaattctc gttcaatcta
    16021 tccagtaatg acttctgccc tgaataattc gtcatggaaa ttttatgcca gaatgcaaaa
    16081 aatcgatccc gcgctggatg tcatatctgg ccaggtgatg agtcatatat tagttgatgt
    16141 gcactacgaa taatatatca aaacaaacga ctgctattat gacagcagcg aatagtgggt
    16201 aatcatgaag acattcttca aatactttgt ttttttagta ctatcctgct cttgctttac
    16261 agcgattagt aaggatacaa gttttcttgt tggtaacaat gccggggtag gcgatggtaa
    16321 tttcaacggc ccgagcactg cggctcaggt cacttttaga tatgcatcaa caaccaataa
    16381 tttagttttt tataaaccta ctcaacttgg cccgacaggt gttaaattac gctgggaaca
    16441 attagataca gccagcggtg gcggttttct ttactgtaat gcctctgaca gggcaaatcc
    16501 agatggacta atggatattg aaaatgccat ggctgattct ggtaaaacct atggtggaca
    16561 taagttattt aaaacctctg tcccaggctt gtattacact ctatttattg caaaactttg
    16621 gtctgcatat tcaacaaaaa caactattag cgactctggt ttatatattg gagataccac
    16681 accacaaaaa ttccagtgga tcatcacaga ccctgatcta caacatatcg gctgcgataa
    16741 cgcgatgcgg tacgatagat tctgggccat cggcggtgtt gtacacgatc ttacgattga
    16801 attttatact gataccgatt ttaaccctac aacaaaccag gacgtatcat tgtccagtaa
    16861 ttcgacatat ctttatgcgt ttaaggccta cagtcccggt tctcgtgttg ttgatcacag
    16921 ccatcacctt tatatcgact ttgatctact caatgttaaa ttaactaacc ccacctgctt
    16981 tactgctgta cttaccggaa aatcagtaag tggttcgacg gtaaaaatgg gagaatatgc
    17041 accagggcag attaaaaatg gtgctactcc ggttcctttt gatatttctc tccagaactg
    17101 tgttcgcgtt ggcgatatag agacaaagct atcttcaggg aagctgggta cagaaaataa
    17161 gcaactcctg ggtaacactc ttactggaag cggcgcagca aaaggcgttg cggtattgat
    17221 tgaaggatta gcaaacagaa aaagtgccct aatgatactg aaaccgaatg attcaacttc
    17281 tgtttataaa gataatacgg gtcaggctca ggatcacgat tcagatgcaa tttacccaga
    17341 agatcaaggt ataacatatc cacttcattt ccaggcgacg ttaaagcaag acggaaatat
    17401 tgctatcgaa ccaggagaat ttaaagccac cagtactttc caggtaacct atccctgata
    17461 acattgtggc accgtctggt aatggcggtg ccacaataga taaatcattg cccccaccaa
    17521 tgtggctgat aaaatgccag acagcacgac attacttatc aggatacgtt atgaaaccaa
    17581 ccactattgc ttcattgcaa aaatgcaaac aagaaaaaaa acgtttcgcg accatcactg
    17641 cttacgatta tagcttcgca aaactgtttg ccgaagaagg acttaacgtc atgctggtcg
    17701 gcgattctct gggcatgacg gttcaggggc atgactccac gctaccggtt accgttgctg
    17761 atatcgccta ccacactgcc gccgtgcgtc gcggcgcgcc aaactgcctt ctgttagcag
    17821 acctgccatt tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtaa
    17881 tgcgtgccgg agccaacatg gtcaaaattg aaggcggtga gtggctggta gaaaccgtac
    17941 aaatgctgac cgaacgtgcc gttccagttt gtggtcattt aggcctaacc ccacagtcag
    18001 taaatatttt cggtggctac aaagtccagg ggcgcggcga tgaagcggcc gatcgactgc
    18061 tcagcgatgc attagcctta gaagctgctg gggcacaact gctggtactg gagtgcgtgc
    18121 cggttgaact ggcgaaacgc attaccgaag cactggcgat cccggttatc ggtattggcg
    18181 caggcaacgt cactgacggg caaatcctcg tgatgcacga cgcgttcggc attaccggcg
    18241 gtcatattcc taaattcgcc aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg
    18301 tgcggcagta tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc
    18361 attgaggagt gacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaatccg
    18421 ccgcctgcgt atggaaggta agcgcgtggc gctggtgccc accatgggta acctgcacga
    18481 cggccatatg aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat
    18541 tttcgttaac ccaatgcagt ttgaccgccc ggaagatctg gcacgttatc cacggacctt
    18601 gcaagaggac tgcgaaaagc tgaacaaacg taaagtggat ttagttttcg cgccttcggt
    18661 aaaagagatc tacccgaacg gtacagaaac ccacacctat gttgatgttc ctggtctttc
    18721 caccatgctg gaaggtgcca gccgtccggg acattttcgc ggcgtttcga ctattgtcag
    18781 caagctgttc aacctggtcc agccggacat cgcttgcttc ggcgaaaagg actttcagca
    18841 actggcgctg atccgcaaaa tggttgccga tatgggcttt gatattgaga ttatcggcgt
    18901 accgattatg cgcgccaaag acggtctggc gctgagttcc cgtaacggtt atctgacggc
    18961 ggaacaacgc aaaattgcgc ccggtctgta caaggtttta agttcgattg ccgacaaatt
    19021 gcaggctggc gaacgggatc tcgatgaaat tattgccatt gccgggcaag aactgaatga
    19081 aaaaggcttc cgcgccgatg atattcagat tcgtgatgcc gacacattgc tggaagtctc
    19141 tgaaaacagc aaacgcgcag tgattctggt agccgcctgg cttggcgatg ctcgcctgat
    19201 cgacaacaaa atggtcgaac tggcgtaatg cttaactggc gctacggctg atggcgccag
    19261 ctattcattg ccacgttatc tgtaagcgat tattcataac gcagacaact tagttaatca
    19321 ttttatcaat ctcagcaaga ggtaaatttg ccatctctgc aacgtcttcc cgagacattc
    19381 ctttactcag aagacgcagg gccagtctta ccttctggag tccctcttta tatcccttct
    19441 gaaatccctt ctcttcaaac cactgcgcca acgtctgcct ggtttctctc cgtgtcttta
    19501 tttccatatg ttcccttaat tatctggtca gttaacaata acaattatct ggctataact
    19561 gataatgcag ataacttagc taatcagctt atcaacctca gcaagaggta aatttgccat
    19621 ctctgcaacg tcttcccgag acatcccttt actcagaaaa cgcagggcga attcctgtct
    19681 tacttcctgt ctaacctctt gtattacctc ctgtcttacc tcctgtctta cctcctgcct
    19741 tcccctctct tcaaaccact gcgccagcgt catcatagac tcccctcccg tttccctgtc
    19801 tcgcaacaca ccataaaaca aatccgcttg ttcagtatga ccgcgttgca gcatatagtt
    19861 ttgcatggca actaactgac ttccgctagt gtacccttcg tcaatcagcg tgaccagttg
    19921 ctccagcaat aacattaagt cgcgctggcg aatatgcttt tgcagtagtt cgagaatcgc
    19981 aatccgccgg tgctgcatga tttcgtcatc cggcgtgatg gtgatatcca ccagcgggaa
    20041 agggttgttg taaacgcgtc gcgccagctc cggcgagtaa aacatatcaa accagcacat
    20101 tgagagcgga taaggtgtgg cctcgccctg ataaaacaga atcggcacca ccagcggcag
    20161 cttatcgtga tccgcctcca gatgccggtg catggcggca atggcatagc gcatcatgcg
    20221 aaaggtcatt ttcttatccg gcttgctttg gtgttcaatc acaacatgca gataacccgt
    20281 actaccctgc atctgtacgg aatagagcac gtcagtgctg tgccctttca ggctttcttc
    20341 aataaaactc cccgactcaa gatgcagcgt gtcgaggtca cacagttcgc gtagctccgc
    20401 tggcaaatgg atatccagaa agtcgcgagc cgtttccgca tgcattaaaa attgtttaaa
    20461 caccgcatca tgcggtgtgg tactcggtgc atccatctcc cctgccttcc gtgttgtctt
    20521 gcgtgaaggc agtatccagt caaggtgtgc cgtcgtcagc cgcctcttta gtgtttaccg
    20581 agcagcgttc agataaattt caattggtta caggcgttac atggcacgct tcggcacggt
    20641 acgcaaactg ctgatgcaaa tgccgtcaga tgagtagaca ctaaacaaaa atcgggcaat
    20701 actgcgtgag aatttcctca aagcaggcat cgcctgcttc gttaacgaca gggtagaaag
    20761 gtagaagtta tgattcgcac gatgctgcag ggcaaactcc accgcgtgaa agtgactcat
    20821 gcggacctgc actatgaagg ttcttgcgcc attgatcagg attttcttga cgcagccggt
    20881 attctcgaaa acgaagccat tgatatctgg aatgttacca acggcaagcg tttctccacc
    20941 tatgccatcg cggcagaacg cggttcgaga attatttctg tcaacggtgc ggcggcccac
    21001 tgcgccagcg tcggcgatat tgtcatcatc gccagcttcg ttaccatgcc agatgaagaa
    21061 gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg acaacgaaat gaaacgtacc
    21121 gcgaaagcga ttccggtaca ggttgcctga ttgttatccc tgcggctggt tactcaccag
    21181 ccgcgacatc gtctccagcg aatctgttct taagatataa agtcgtttta gtaacaacgg
    21241 attatccccc ggttttactt tgcctttcat ggtcgtcaca gccaggtgaa atccggcttc
    21301 ttttgctgcc ttcacggctt tgtcattaaa tccgccaaat ggataagaaa gatacaaaac
    21361 atgtggatta aattgcgcca gagcgcggcg tgaacgtgca aaatcaaaca gaatattgtg
    21421 ctcactacgg ctcagcaata taggccgccg ataaccatct atccgatgca aaaaatgagt
    21481 atgtgactgg aaatcaaata catcgcgaat ttcgttaagt tcagaaacgc tcataaattg
    21541 cagcgatttt gggttccatt tctgcgggtg acgtttgatg cgcgaggtaa caataaacgc
    21601 cgtcgccttc atgccatatt gtttcaacac aggatacgca tagcggctca ccgacttgag
    21661 gccatcatca aaggtaatca ccaccgctcg cgcagggaga ttgatcttat tcttcacgta
    21721 accttccagc tgcgccatgc tcagtgtcgc gtatcccctg tcgcgcagcc aggccatctg
    21781 gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg cgaaaacggg tgttttcttc
    21841 atcgcgcaaa atatggtgat aggttagcac cggcaggcca ttatcgggtt gggcatccag
    21901 cgcgctgata taggccagtc gatcgccaat acggatctga taccaggttt gatttaacct
    21961 gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc gccagtactc caaatggcgc
    22021 actgcccaca ctcggcgcgt tatagacctg cgtatctttc caggtaacta agttctgatt
    22081 actcagcggc ttgttgaggt cacccaaacc gtcttcaact ttttgtcgcc cctgaaccgg
    22141 ctcgagatgc cctttatcga taaacccttt gccaaggcca aaattaaata cgtaataact
    22201 tgcggcagtg ggctccacgg caatgatttg tccggcccga atattcccca ccgtcaccat
    22261 cttgtcacca atttgcgccc agaccgcggc attttcgatg gtttgcatat aacgggcagg
    22321 taacgcggca ctgacacttg cggtaaacag catcagcagg agaataacag cttgtttgta
    22381 catgaaaaaa taaccgaggg tgaaacaacg cttttattgt agcaaaagcg tcatcagaat
    22441 cgcgggttaa tactcataca aattatgttt gaggacgaaa ggcggatttt tttgttgctg
    22501 gtgccaaaga ctactcacct ccggcgcacc cagttcgaca atacgctcgc gaaacgctga
    22561 actggtcatg gtttcacggc aagccatcat ctgttcaatt aacggtaacg tgacaccaga
    22621 aatcacttcg caacgggagt gtttgtggct taataatgaa gccacgcggt acggtggcgc
    22681 tccggctata tcggttaaga aaataacgcc ttcaccagag tccgtttcat gcaaggcatc
    22741 gcacatcatg cggctgagca tattagagct taatccgcgc cagaaattca cggcccggca
    22801 ctgaagaagt gccccatgtt ttttctccag cgcatccagt atctcttgcg cccggtcatc
    22861 gtgacaggta attacccaac ctaacatcgc cttcctcctt agccgaaaat cagtctaccc
    22921 tgaccattgt tgataaaggg tgatagccgt caaaactcct ctcccccaaa tccagaggag
    22981 aggaaatagc cttagctacg caaaccacgt ccacgttgga tcagcgacca acagatcaaa
    23041 taaaacgcca caataaagac caccagtacg ccaaaggtag tgaccagcgg aacatcattg
    23101 ataccgagga agccgtagcg gaaaccactg atcatataaa cgattgggtt caggtgcgac
    23161 agcccttgcc agaacggcgg caacaaagtc agggagtaaa acaccccgcc caaatacgtg
    23221 agtggcgtta acacaaaggt tggcaccagg ctgatgtcat cgaacgtttt agcaaacacg
    23281 ccgttcagca aacccgcaag ggagaatagc accgcagtga gcaccagggt taaggcaacg
    23341 aatacccacg aatgcacctg aaacggcaca aaaaacagtg aaattgccgt caccagaata
    23401 ccaacaaaca gaccacgcgc cacgccacca ccgacatatc cggcaataat gacatgagtc
    23461 ggaaccggcg ctaccagcag ctcttcaata ttacgctgga acttggcacc aaaaaatgat
    23521 gacgcgacgt tagcgtaggc gttggtgatc accgacatca tgatcagccc cggtacgatg
    23581 aactgcatgt agctgaagcc atgcatatcg ccaatacgcg aaccaatcag gttaccgaag
    23641 ataataaagt aaagggtcat ggtgatgact ggcggcacca gcgtctgcac ccagatacgc
    23701 ataaagcgat ggatctcttt cgcccagatg ctttttagcg ccacccagta aagatgcatc
    23761 atgtgcgatc tccttgtttt tcattaacca gtgaaacaaa cagctcttcc agacggttag
    23821 ctttgttacg catacttaat acctgaatgc cctgctcact taactgcgta aatacgctgt
    23881 tgatgccctg ctcacgcagc acttcaactt ccagcgtggc ggtatctacc agtcgatact
    23941 gatagccatc gagcttcggt aacggacttt tcggtgcgag atcgagaata aaggtttctg
    24001 atttcagctt cgccagcagc gccttcatcg aagtattttc caccaactca ccgtgttgaa
    24061 taatgccgat attgcggcac agcatttctg cttcttccag gtagtgtgtg gtgagaatga
    24121 tggtggtgcc tttgtcgttt aaatccttca aaaagcccca cattgagcgg cgaagttcaa
    24181 tatccacgcc tgcggtcggt tcgtcgagaa tcagtagttt aggttcatgc attaacgcac
    24241 gggcaatcat taaacggcgc ttcatcccgc cagataacat acgcgcacgt tcgttgcgtt
    24301 ttccccatag atcgagttgt ttaagatact tttcgctgcg aatgtacgct tctttgcgct
    24361 ccacaccgta gtaccctgcc tgattcacca caatttgctg cacggtttca aacgggttga
    24421 agttaaattc ctgtggcacc agacccaact gacgtttagc gtttacgaca tctttctcga
    24481 gatcgtaacc aaatacgctg acccgcccgg aggttttatt taccagagag ctgataatac
    24541 cgatagtggt cgattttccg gccccgttcg gcccgagaag cgcataaaaa tcacccgctt
    24601 cgacctgcaa atcgattcca cgaagcgcct gaacgccgcc tggataggtt tttttaagct
    24661 gttgaagttc cagtgcaatg gtcataaatt tttacttacc ttacgttctt acactttatc
    24721 tgtggtttaa atcgtcccgg agttgcccta tattagccaa acgtaattat ttggttacag
    24781 gtcgttaacc tccatgaaag acatagatac actcatcagc aacaatgcac tatggtcaaa
    24841 aatgctggtg gaagaggatc ccgggttttt tgagaaactg gcacaagcgc aaaaaccgcg
    24901 ctttctatgg attggatgtt ccgacagtcg cgttcctgca gaacgtttaa ccggtcttga
    24961 gccgggcgaa ctctttgttc accgtaatgt tgctaacctg gtcattcaca ccgacctgaa
    25021 ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa gttgaacaca ttattatctg
    25081 tggccactac ggttgcggcg gcgtacaagc cgcagttgaa aacccggaac tggggcttat
    25141 caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat tgctcggcga
    25201 aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg aacaggtgta
    25261 taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga aagttaccat
    25321 tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg tcacagcgac
    25381 taaccgcgaa acccttgagc aacgttaccg tcacgggatc tccaacctca agctgaaaca
    25441 cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact tcacacttac
    25501 tcgtccagca gaatcacttt gccgatatac ggcagatgac ggtaacgctg tgcgtaatca
    25561 atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc taccgggacg
    25621 ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga cttcggttcg
    25681 cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat gatatcttca
    25741 acaatcagca cgtccttgcc acggatatct tcatccagat ctttgaggat tttcacatca
    25801 cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac ttcatgagat
    25861 acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag cagacccacc
    25921 agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc cagttcggcg
    25981 atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt catatctata
    26041 accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg caacaaagcg
    26101 caagacacgc agtataccag taaaactatt cattcgcgga atctgttaat aaagctcgaa
    26161 ttgtgatgac gatcacacat gttaaaacct ataattaatt gctactaaaa tattaatgaa
    26221 ttgaaatggt gtctctttat ggcaattaac aataccggct cgcggcgatt actcgtcacg
    26281 ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg aggctggctg
    26341 gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct cagcgtcgcc
    26401 tggatgctgt ggcgcagtaa acgcgccgcg ctttggctgt acgcagccct gctgctcggc
    26461 actatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac tccgcgcagc
    26521 gacattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg tcgcctggtc
    26581 attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag cggtggtatc
    26641 ctgacctggg ccggatttaa cgatccgcag gaaatcagcg gcaccttaag cgccgatacc
    26701 acgccagcag aagccatctc ccccgttgcc gatcaggact ggcctgccta tggtcgcaat
    26761 caggaaggtc aacgcttttc acctctgaaa caaattaacg ctgataacgt ccacaagctg
    26821 aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc gggcgaaatc
    26881 accaatgaag tgacgccaat taaagtgggc gacacccttt acctgtgtac cgcgcaccaa
    26941 cgtctgtttg cgctggatgc cgccagcggt aaagagaaat ggcattacga tcctgagctg
    27001 aaaaccaacg agtccttcca gcatgtaacc tgccgtggcg tctcttatca tgaagccaaa
    27061 gcagaaactg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat tcttccggtc
    27121 aatgatggtc gtctgattgc aattaacgct gaaaacggca agctgtgcga aaccttcgcc
    27181 aataaaggcg tgctcaatct gcaaagcaat atgccggaca ccaaaccggg tctgtatgag
    27241 ccgacttcgc cgccgattat caccgataaa accatcgtga tggccggttc agtcaccgat
    27301 aacttctcaa cccgtgaaac atctggcgtg atccgtggtt ttgatgtcaa caccggggag
    27361 ctgctgtggg cttttgatcc gggcgcgaaa gatccgaatg cgatcccgtc tgacgaacat
    27421 acctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc gaagctggat
    27481 ctggtctatc tgccgatggg cgtcaccacg ccagatattt ggggtggtaa ccgcacaccg
    27541 gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg gaaactggcg
    27601 tggagctacc agaccgttca ccacgacctg tgggatatgg atcttccggc acagccgacg
    27661 ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc ggcgaaaacc
    27721 ggcaacattt ttgtgctcga tcgtcgtaat ggtgaactgg tggttccggc cccggaaaaa
    27781 ccggttcccc aaggtgctgc caaaggcgat tacgtgaccc ctactcaacc cttctcggaa
    27841 ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggtgc caccatgttt
    27901 gaccagttgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat ttttacgccg
    27961 ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga atggggcggg
    28021 atttccgttg atccgaatcg tgaagtggcg attgccaacc caatggcact gccgtttgtt
    28081 tcgaaactga tcccacgcgg tccaggcaac ccgatggaac agccgaaaga tgccaaaggc
    28141 acgggtacgg aatccggcat ccagccacag tacggtgtac cgtatggtgt cacgctcaac
    28201 ccgttcctct caccgtttgg tctgccatgt aaacagccag catggggcta tatctcagca
    28261 ttggatctga aaaccaatga agtggtgtgg aaaaaacgta ttggtacgcc gcaggacagt
    28321 atgccattcc cgatgcctgt accggtgccg ttcaatatgg gtatgccgat gcttggcggg
    28381 ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa ctacctgcgc
    28441 gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccagc gggtggtcag
    28501 gctacgccaa tgacctatga agtgaatggc aagcagtatg tggtgatctc cgcaggcggt
    28561 cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgcgct gccggatgat
    28621 gtgaagtaac cataatgccc ggagagatcc gggcatattt ccgaatacgg tctttttata
    28681 ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag tgcgccatat
    28741 aagcatgttc tttcggtgca tcgtgattaa acttcaccag cacttcgctg acattacctt
    28801 ctaccttaac ggtatctttc cagcctgcgc gatgcgtcgc tggcggtttg ccattttctg
    28861 acaagatacg gaactgcgta ccgtggatat ggaacggatg cagcatcatg tcgcccacgc
    28921 cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc ttattcatat
    28981 caaacgcctg accgttgatt ttgttggcat ggtggaaatc gaacttcccg ctgtggttca
    29041 tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc atcccggcca
    29101 tggcctgatc gccatatttc tccatcagca tctgcatccc catcatatcg agcattgggt
    29161 ccatagaaag ttgcagattg cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc
    29221 tacttaatgt gtctggcaaa gcaccagagg cactaatggc aatcggctga atccgcatta
    29281 ccggatgcgg cttgtcaaac ggcgcaatcg ccatccccat ctggctgacc ggcagcgtca
    29341 ccaggtcaaa gggtttgtta tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca
    29401 gcactggcag ctcgttcacc ttcaccggtt caggtagcag accgccgtcg ctggcaatca
    29461 cataaagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta cagccattga
    29521 gcaaacgcag gcgcagccag ccacgcgggg cagcgtgttg cgggtagatt gcaccgttgg
    29581 tcagcaacgt atcgccaaac caacccacag cggcggtcat cacatccagt tgataatcaa
    29641 tctgcccgtc tgcgttaaat tttttatcct gaacaatcac cgggacatca tcaatacccc
    29701 actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc aaaccagcca
    29761 gccccatcgc cacctgacgt ccggtcttgc catgttgatg tggatggaac cagcaggtag
    29821 cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc acctggcgga ataattccct
    29881 gcgggccgcc gtccacttca cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt
    29941 ccgtcagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg
    30001 gccccagcag attgccgtta tagccccagg tagttgcggt tttcccgcca aaggtggact
    30061 gacctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga
    30121 ttggtaacgt tgggcgttcc gccgcaaata ctgcgcggct ccacagcggc aaggctgaag
    30181 ccacccccag cgcgacggaa tatttcaaga aatcacgacg ttgcatagtt atttccttat
    30241 tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta cgggaaggtc
    30301 aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac
    30361 gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa cagtgttatt
    30421 cggcagcctg atggccgttt gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga
    30481 tatggccgat ttaacggcag tttttgtttt tctgaagaac gattgtggtt accagaactt
    30541 acctaacggg caaattcgtc gcgcgctggt ctttttcgcc cagcaaaacc agtgggatct
    30601 cagtaattac gacaccttcg acatgaaagc cctcggtgaa gacagctacc gcgatctcag
    30661 tggcattggc attcccgtcg ctaaaaagtg caaagccctg gcccgcgatt ccttaagcct
    30721 gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc gtgcatccgc
    30781 ggtcaatgtt agctattatg ttgcgccatt tttttacggg tgttaacaaa ggaggtatca
    30841 acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg ggcagtactt
    30901 tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga tcatttttga
    30961 gaacgccgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca ccgagcgcga
    31021 cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa
    31081 acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa
    31141 aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtgtcgt tctgccgtca
    31201 gtatcttccc aaccataacg ccggtagtta cgacgatccg cgctttaagc tggtgatcga
    31261 cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta tctccgactg
    31321 caccgatcct atcggtcccg gcgaaagtct gttcacttcg gcattttatg aaggctgcaa
    31381 acgttgcctg aatcctggcg gtattttcgt tgcgcagaac ggcgtctgct ttttacagca
    31441 ggaagaggcc atcgacagtc atcgcaaact cagccattac ttcagcgatg ttggctttta
    31501 tcaggcggcg atcccgacct attacggcgg catcatgacc tttgcatggg cgacagataa
    31561 cgacgcctta cgccatcttt caaccgaaat tattcaggcg cgttttctcg cttctggcct
    31621 gaaatgccgt tattacaatc cggcagtcca tacggcagct tttgccttac cccagtatct
    31681 gcaagatgca ctggcttcac agccgtccta aggagaagat aagaaattga aaaaactgaa
    31741 actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg atatctgcta
    31801 cgccaaaact accgaagagc gcgacggtta tattgcttat atcgatgaac tctataatgc
    31861 caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca atattcttaa
    31921 catcgcccgc caggattacg aaccacaagg tgccagcgtc actattctgg tgagtgaaga
    31981 accggtcgac ccgaaactta tcgacaaaac agaacacccc ggcccactgc cagaaacggt
    32041 cgttgcccat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa gtcatcctga
    32101 aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg tgatttctcc
    32161 gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatattgtga ctattgatta
    32221 tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag cactttatcg accatgagat
    32281 taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt
    32341 gaacgtctat caggagaata tcttccatac caagatgttg cttaaagagt tcgaccttaa
    32401 gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc aggaaattac
    32461 cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc ccgctgttta
    32521 acgtctcagg cggagcacgg aggctccgcc tgatttactt acttctgctg cacgaaatta
    32581 cggtaagccg ccaccacctg aagaaaatcc tcaacgccac acagtgacaa gctctcttca
    32641 tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg attggcgcga
    32701 accatcacct cttcaccatc catccatagc gtgtattcat gccctgcccg ttgccaggaa
    32761 cgttcgctac ctttcaatgc gtgcgccgct tgttccactt catcaagcaa ggccaggttt
    32821 tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga catgcgcacc
    32881 tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc
    32941 ggcaatgcgc cgaaatcgca ttgcgccatt gatgtaatta tcgcagtctt accggagaaa
    33001 aacagcggaa cggcgatggt ttagaaataa aaaatgcccg ggaatctcat tcaccgggca
    33061 ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaaa ccgcagtctg gaaaatcacc
    33121 ccgtccgctt tttcggtgta ctgagaaagc tggtcgaagt tcagataacg gtaagtatca
    33181 acggcagttt tatctacttg cgccacgtag gtctggtact cttccggcgt cggcagtttg
    33241 ccaatcagcg ccgcaacagc agccagttcc gcagaagcca ggaagacatt cgcgccagta
    33301 cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc gtctgctaca
    33361 cgcgcctggt tacccataca cagggaacag ccagggatct cgatacgagc accactctta
    33421 ccgaagacgc tgtagtagcc ttcttcggtc agctgcgcag cgtccatacg cgttggcggt
    33481 gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt accagcagca
    33541 cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat cttctcgccc
    33601 tgtaccgcag acagcggacg tgcgtcgtcc gggtcattcg gtgcacagag gattggctct
    33661 ttgatatccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc atcggcttcc
    33721 agcagctcag gattcgccag ccatttttcc atgccctgaa tacgacgctc cagggtacga
    33781 cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttaga gttcaggtat
    33841 tcaatgatcg gttctttgtt cagcttgatg gtacaaccgg cagcagaacg ctcggcagac
    33901 gcatcggtca gctcaaatgc ctgctcaact ttcagatccg gcagaccttc aatttccaga
    33961 atacggccag agaagatgtt tttcttacct ttcttctcaa cggtcagcag accttgtttg
    34021 attgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatacccgg ctgcattttg
    34081 cctttgaagc gtaccagaac ggattccggc atatccagcg gcattacgcc tgttgcggct
    34141 gcaaatgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa acgggtatgg
    34201 gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca ggagtgaatg
    34261 acgccgtccc ccggacgcag cgacacaccg ccacggttca taatgaagtc cggcagcgtg
    34321 tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa agactgcatc
    34381 accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg ggtcatcggg
    34441 ccggtggtgt cctgggaacc tacagaagtc attttcggtt cacagtacgc acccggacga
    34501 atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa gccgcgatcg
    34561 ctctcagcga catctttcgc ctgacggaac acatcactat gcggcaggcc aagtgcttca
    34621 cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc agcacgcact
    34681 tcatcaatca gcacgtcggt tttcagttcg aaggttgcca gcagttcgcc ggtttcgtgg
    34741 ttacgcactt cacctttgta cgggtaaacg tcaattacgt cgcccatgtt caggttagag
    34801 acgtcgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa gattggtgca
    34861 attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg aatatcatcg
    34921 cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaccccgt acccacaacg
    34981 tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg tttgatcggg
    35041 ccaacaacac caggctgatc tggctcaata ccttcacggg cgtttttcag catcgccagc
    35101 gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag gtcatcggtg
    35161 ttggtttcgc cggtcacttt gaagacggta acggtcagtt tttcagccag cgccgggcga
    35221 ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata ttcgttacct
    35281 gctttggctt tctcttctac gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt
    35341 gctttggcag cgatcggtgc cagtttggca tcatccagcg cgtcgatcag cggatgaatg
    35401 ttgtaaccac cctgcatagt gcccagcagt tcaatggctt tttccggagt cagcagtggg
    35461 gatttggctt cacctttggc gacagcagcc aggaagcctg ctttgacata ggcggcttca
    35521 tcgacgccag ggggaacacg gttggttaac agatctaaca ggaattcttc ttcgcccgcg
    35581 ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag gggtttgggc
    35641 gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag cacgacggtt
    35701 ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct gtgagacagc
    35761 agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt tgacgatgtg
    35821 ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg atggtctaga
    35881 gattatttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc tttctcaacg
    35941 aatcccaaga ttagtcaaaa tttaaactac ggcctcttta tactcggatt cacagcacct
    36001 gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg aaaatgactt
    36061 tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctctgt gccgcctctg
    36121 ccgggctata tataaaaagc cgcacagtgg aagcgcctgt ggaagcgcaa tcgacacaac
    36181 agactgcgcc tgacatctcc gcagttacgc ttccagcaac ggtttccgtg cctcctgtaa
    36241 cacctgccgt cgtcaaatcc gcattcagca ctgctcaaat agatcaatgg gttgcacctg
    36301 tcgcgctata ccccgactct ctgctttcac aagtgttaat ggcatcaacc tatccggcaa
    36361 acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc gatgctgcta
    36421 ttcaggcggt atctgaccag ccctgggacg ccagcgtcaa atcactggtg gcctttccac
    36481 agttgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc gatgcttttc
    36541 tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg gcgcaacaaa
    36601 ccggctcgct gaagtcatca actgaacaaa agatcatcac gacaacgaaa aaagtcgtac
    36661 cggtaaatca gcccgccaac gcccctgcca cacaatcaaa taccgtttct acgtccagcc
    36721 ccgtcgtcgc agaacctgca ccgaccgtaa taaccattga gccagccaat ccggatgtgg
    36781 tttatattcc gaactacaac ccaaacgtgg tttacggcag ttgggccaat accgcttatc
    36841 caccagttta tctgccgccg ccagccggag aaccgtttgt tgacagcttt gtgcgcggat
    36901 tcggctatag catgggcgtt gctaccacat acgcactatt cagcagcatc gactgggacg
    36961 acgacgatca tgaccatgac catcatcatc atgacgatga tgattatcat caccacgatg
    37021 gcggtcatcg tgacggtaat gactggcaac acaacggcga caacatcaat atcgacgtca
    37081 acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca
    37141 atccaaacta ccgtaatggt gtgacctatc atgatcagga tatggcaaag cggtttcatc
    37201 aaaccgatgt caacggcgga atgagcgcca cgcagttacc tgctctatcg cgcgacagcc
    37261 agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacgc
    37321 gagataccca acgtcaggca gcggcacagc ggtttaatga agcggaacac tatggcaatt
    37381 atgacgactt ccgcgagttc agccgccacc aaccactgac ccagcagcaa aaggacgccg
    37441 ctcgccagcg ttatcagtct gcctcacctg agcagcgcca ggcggcccgt gagaaaatgc
    37501 agactagccc gcagaatcaa cagcaaagag atgcagcacg tcagcgtatt cagtccgcgt
    37561 cgcctgagca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaagagc
    37621 aacgtgataa cgcccgccag cgtgttcaat cggcatcacc tgaacaacgt ccgttttttc
    37681 gggagaaagt tcaggagagc cgcccgcaac gtctaaacga cagtaaccgt actgccaggc
    37741 tgaataacga gcagcggtca gcagtacgcg aacgtctctc tgaacgtggg gcaagaagac
    37801 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    37861 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcggca ggtcggtaat
    37921 gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg ggtgcgcatg
    37981 gatggtcagt gcgatgtctt cagcgtcaca acccatttcg attgccaagc cgatttcacc
    38041 cagcaactcg ccgccgttag tgccgacaat cgcaccaccg atcacacggt gagattcttt
    38101 gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa gcgatagcac gaccagaagc
    38161 agcccacggg aaggtggcgg tttcgtagct gatgcctttc tctttcgctt ctttctcagt
    38221 cagacctacc catgcaactt ctggttcggt ataggcgatg gacgggataa ctttcggatc
    38281 gaagtaatgt tttttaccgg cgataacttc agcggcaacg tgaccttcgt gaacaccttt
    38341 gtgtgccagc attggctgac cgacgatatc gccgatagca aagatgtgcg gtacgttggt
    38401 acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtca acttccacgc cagctttgcc
    38461 tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg cgtcgtaacg
    38521 ctgcggttca gcgggtgctt ttttgccttc catcgtcaca taaatgccgt cttctttcgc
    38581 ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga tacgcttggt
    38641 aaagacttta acgatgtctt tatcagctgc cgggatgacc tggtcgaaca tttcaaccac
    38701 gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt tccagaccga tgataccgcc
    38761 acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag tggagtccca
    38821 gatacgcgga tcttcatgcg gaataaacgg cagttgaatc gggcgagaac ccgctgcaat
    38881 gatcgcgttg tcgaagttga tcacggtttt accgttctca ccttcaactt ccagggtgtt
    38941 agccccggtg aatttaccca gaccgttgac cactttgact ttgcggcctt tcgccatacc
    39001 agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa tcttgtcgat
    39061 atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg cttcttcgat
    39121 aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca ggcaaacacc
    39181 gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag cgcaacggaa
    39241 ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt tgatttcagt
    39301 actcatcatg acctctatat atttatctcc ggcgggtctg acgttttttt tatgacgccc
    39361 attatccacc gggtcgttct atccgtcggt attttacaaa attgttaaca att
//
LOCUS       DAOFSV010000032        39395 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.032, whole genome shotgun sequence.
ACCESSION   DAOFSV010000032 DAOFSV010000000
VERSION     DAOFSV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.032"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(10..366)
                     /locus_tag="QBH27_001757"
     CDS             complement(10..366)
                     /locus_tag="QBH27_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDQ7014079.1"
                     /translation="MTILELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(370..786)
                     /locus_tag="QBH27_001758"
     CDS             complement(370..786)
                     /locus_tag="QBH27_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDQ7014080.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(897..1610)
                     /gene="aphA"
                     /locus_tag="QBH27_001759"
     CDS             complement(897..1610)
                     /gene="aphA"
                     /locus_tag="QBH27_001759"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDQ7014081.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1887..3707
                     /locus_tag="QBH27_001760"
     CDS             1887..3707
                     /locus_tag="QBH27_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDQ7014082.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEKHE"
     gene            3700..5058
                     /locus_tag="QBH27_001761"
     CDS             3700..5058
                     /locus_tag="QBH27_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDQ7014083.1"
                     /translation="MNNKFSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(5047..6120)
                     /locus_tag="QBH27_001762"
     CDS             complement(5047..6120)
                     /locus_tag="QBH27_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HDQ7014084.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     TKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(6132..7631)
                     /locus_tag="QBH27_001763"
     CDS             complement(6132..7631)
                     /locus_tag="QBH27_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDQ7014085.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSSAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            complement(7843..8715)
                     /gene="sucD"
                     /locus_tag="QBH27_001764"
     CDS             complement(7843..8715)
                     /gene="sucD"
                     /locus_tag="QBH27_001764"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDQ7014086.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKDAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHDMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            complement(8728..9897)
                     /gene="sucC"
                     /locus_tag="QBH27_001765"
     CDS             complement(8728..9897)
                     /gene="sucC"
                     /locus_tag="QBH27_001765"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDQ7014087.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEASITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            complement(9919..11337)
                     /gene="lpdA"
                     /locus_tag="QBH27_001766"
     CDS             complement(9919..11337)
                     /gene="lpdA"
                     /locus_tag="QBH27_001766"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDQ7014088.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLVVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            complement(11349..12503)
                     /gene="odhB"
                     /locus_tag="QBH27_001767"
     CDS             complement(11349..12503)
                     /gene="odhB"
                     /locus_tag="QBH27_001767"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDQ7014089.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPAIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVHTLVAIRELLESPEQLLLDL"
     gene            complement(12536..15280)
                     /locus_tag="QBH27_001768"
     CDS             complement(12536..15280)
                     /locus_tag="QBH27_001768"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDQ7014090.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNISGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWASSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDDAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            complement(15462..16655)
                     /gene="tyrB"
                     /locus_tag="QBH27_001769"
     CDS             complement(15462..16655)
                     /gene="tyrB"
                     /locus_tag="QBH27_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDQ7014091.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESSVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNEQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLVEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDQLREEFGVYLIASGRMCVAGLNTQNVHRVAKA
                     FAAVM"
     gene            complement(16737..17741)
                     /locus_tag="QBH27_001770"
     CDS             complement(16737..17741)
                     /locus_tag="QBH27_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ7014092.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATFLVLTPELVARYNLPAIPHNNADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALAGVLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            complement(17738..18295)
                     /locus_tag="QBH27_001771"
     CDS             complement(17738..18295)
                     /locus_tag="QBH27_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDQ7014093.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIVTSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWYVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWYGLYQWRKELQTS"
     gene            complement(18318..19397)
                     /gene="alr"
                     /locus_tag="QBH27_001772"
     CDS             complement(18318..19397)
                     /gene="alr"
                     /locus_tag="QBH27_001772"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDQ7014094.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEANLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGAPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(19450..20865)
                     /gene="dnaB"
                     /locus_tag="QBH27_001773"
     CDS             complement(19450..20865)
                     /gene="dnaB"
                     /locus_tag="QBH27_001773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDQ7014095.1"
                     /translation="MAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            complement(20938..21972)
                     /locus_tag="QBH27_001774"
     CDS             complement(20938..21972)
                     /locus_tag="QBH27_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ7014096.1"
                     /translation="MAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQK
                     VQQAIEQLGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITV
                     MSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDW
                     QQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPW
                     STDAFSLRAEPTQANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGED
                     ISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQ
                     IRASTGKRGR"
     gene            22228..23799
                     /locus_tag="QBH27_001775"
     CDS             22228..23799
                     /locus_tag="QBH27_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDQ7014097.1"
                     /translation="MCSVRTITAPEAADLIVDGAVVTVSSSSGMGCPDAVLAAIGERF
                     QRAGHPQNITSIHPIAAGDMYGVKGIDHLAQPGLLAKVIAGSYPSGPSSMTPPLIWQM
                     INDNAIPAWNLPSGILFDMHREAAAHRPGVLTQTGMDTYVDPLLQGGAMNEKAAQQSL
                     VERVRFANDDWLFFPSIVPQVAIIRATTADERGNLTYEHEGAYLGPLEQATAVRNNGG
                     IIIAQVKRQVAAGSLKPKEVRIPGVLVDYIVIAPEQTQTTQTQYEPAISGEISRPLSA
                     FRYMEHGPARVIAQRVAQELQSGDAVNIGFGISANVPRILLEQGRHGDVTWLLEQGAI
                     GGVPLLEFQFGCASNAEAFLPSPQQFTYFQGGGFDLTLMSFLQIGADGSVNVSHLPAR
                     PHVTAGCGGFIDITSHAKRIIFSGFFNAGAQLQLEEGQLRIIKEGKAKKLVQDVAHVT
                     FSGKRAIRLGQQVQYITERCVLELTPDGLLVTEIAPGIDPERDILAQSDAPLRLADDL
                     KLMNPLYFQQGSHHE"
     gene            23792..24571
                     /locus_tag="QBH27_001776"
     CDS             23792..24571
                     /locus_tag="QBH27_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDQ7014098.1"
                     /translation="MNNGTIDLQRNGFIATLVINRPAKLNALTPEMLEQLLAHCHTLE
                     TDSEIRVVLLTSASEKAFCVGADIHRWTSLSALDMWRNWIRRGHQAFDALAQLPQPVI
                     ALLHGLAYGGGLELAATADIRICSAEAQMALPETGLATVPGWSGTQRLTALLGRSLVK
                     ELVFTGEPLDAQRALSCGLVNRVVAREALHDAGLQLAQRIAQRAPVAVQIAKQVIDAG
                     EGIAVASTLEALAGAFSATTEDAHEGSQAFTEKRQPHFQAR"
     gene            24598..25878
                     /locus_tag="QBH27_001777"
     CDS             24598..25878
                     /locus_tag="QBH27_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDQ7014099.1"
                     /translation="MNMTTSVPQTPSLFRRIMTKLAAFAPGIFLIGYSIGTGSITTMA
                     KAGANYGVSLMWAVALSCIVTWFLIRIYGRYASVTGERALTAFRRHIHPGVAMLFLIG
                     LTVAVSGSIMGVMGILADVMATWSTTWFSGGISALAWAIAMVIMINVMLWTNSNAGFD
                     KILSALVGIMGLAFVINFFIMMPPVADLLAGFIPSVPDPVAGDNSTPFLIISGMVGTT
                     VAPAMFLMRTTLVKSNGWGVDNLPHQNRDAIVSAGMMFIISAAVMAAAAGTLYQTGTG
                     LNNASQMVIILEPIAGSAAVGLFVIGIVAAGLSSQFPNLLLTPWLLADWRDTSNEVSP
                     GYRILMTVMSLLCLVVPLFHARPVLVMIASQAFNAIILPLTVLCMFWLANKPSLMGKY
                     RCSRWENVMFIAISVFTLIMGYMAMSGLIESLMK"
     gene            25889..27925
                     /locus_tag="QBH27_001778"
     CDS             25889..27925
                     /locus_tag="QBH27_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDQ7014100.1"
                     /translation="MIDETLTALTDIKMPHYAPLAQASGSIDLNGTILPEYCCNTLVL
                     GSGAAGWRAAVELKRQNVDVMVASSKAFWGTSACSGSDKQTLHTANTRANGDHFLTLS
                     NTLAAGGAMDHDTAYVEAVGSVNTCEVLKYLGLDLPEDLFGATLRYQTDHDEFGRATS
                     CGPRTSRLMVKVLAQEAMRLNIPLCDHTTAIHILTSGEGENRRVVGVIAIDKACRDNP
                     WRMVVIRCQHLVLATGGPGELYRDSVYPVNCFSALGMALEAGITLVNLTESQFGIGTP
                     RSQFPWNLSGTYMQAMPRIYSQDHSGRQYNFLAAYYPTTRMLASAIFRKGYQWPFHAE
                     RMLEYGSSLLDVAVYEETQKGRDVFLDFLREPEPAADGTSFNLQELDDDVIDYLQQNH
                     ALLAQPLARLTQMNPLAIRLYQMHGHDLTASPLKFTLNNQHLNGGIEVDIWGRTSLTG
                     CYAAGENAGTHGVTRPGGAALNAGQVFARRCALHIAHQKSQDRPLERQQILSTINEAQ
                     QYLNQGLLRDDVRETIQNTMSHHAAILCHTQGINTAAQTLATLCEDIRREGIQCDENS
                     LAQSFQWRQSAQLALAVLRSLQCYVENGGGSRGARAIYDGEAGIAPQTPGGPLLAWRF
                     RPENQDARQYMICVCREANDYQTWTRPCRERGVLTLTNFERQWQNWIAATPFQY"
     gene            28031..29014
                     /locus_tag="QBH27_001779"
     CDS             28031..29014
                     /locus_tag="QBH27_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDQ7014101.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVKNKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSMHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(29179..29421)
                     /gene="pspG"
                     /locus_tag="QBH27_001780"
     CDS             complement(29179..29421)
                     /gene="pspG"
                     /locus_tag="QBH27_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDQ7014102.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQHYDRWRY"
     gene            complement(29555..30550)
                     /gene="dusA"
                     /locus_tag="QBH27_001781"
     CDS             complement(29555..30550)
                     /gene="dusA"
                     /locus_tag="QBH27_001781"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDQ7014103.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            30706..31221
                     /gene="zur"
                     /locus_tag="QBH27_001782"
     CDS             30706..31221
                     /gene="zur"
                     /locus_tag="QBH27_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDQ7014104.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(31263..31472)
                     /locus_tag="QBH27_001783"
     CDS             complement(31263..31472)
                     /locus_tag="QBH27_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDQ7014105.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(31588..32913)
                     /gene="dinF"
                     /locus_tag="QBH27_001784"
     CDS             complement(31588..32913)
                     /gene="dinF"
                     /locus_tag="QBH27_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDQ7014106.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(32986..33594)
                     /gene="lexA"
                     /locus_tag="QBH27_001785"
     CDS             complement(32986..33594)
                     /gene="lexA"
                     /locus_tag="QBH27_001785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDQ7014107.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(33704..34072)
                     /gene="dgkA"
                     /locus_tag="QBH27_001786"
     CDS             complement(33704..34072)
                     /gene="dgkA"
                     /locus_tag="QBH27_001786"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDQ7014108.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            34243..36666
                     /gene="plsB"
                     /locus_tag="QBH27_001787"
     CDS             34243..36666
                     /gene="plsB"
                     /locus_tag="QBH27_001787"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDQ7014109.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTKQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(36821..37693)
                     /gene="ubiA"
                     /locus_tag="QBH27_001788"
     CDS             complement(36821..37693)
                     /gene="ubiA"
                     /locus_tag="QBH27_001788"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDQ7014110.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARTLFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(37706..38203)
                     /gene="ubiC"
                     /locus_tag="QBH27_001789"
     CDS             complement(37706..38203)
                     /gene="ubiC"
                     /locus_tag="QBH27_001789"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDQ7014111.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(38382..39302)
                     /gene="malM"
                     /locus_tag="QBH27_001790"
     CDS             complement(38382..39302)
                     /gene="malM"
                     /locus_tag="QBH27_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDQ7014112.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVSGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSVPDIPDPIARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         9890 a        10405 c         9641 g         9459 t
ORIGIN      
        1 cttcttcttt cagagttgca ccagcaattt acgtttttct tccggcagca aattcaccgc
       61 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg atccgtcgag
      121 atacacggtg ctccagtgtg ctttgttcag atggcggctt ggacgcacat cgctgtgctg
      181 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
      241 tacttctttc accatcgcaa acagcacatc ttccactttg atctgcgtcg ctttccagtc
      301 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccaaaat
      361 ggtcattttt tactcccctt gaagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
      421 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgcaccgg
      481 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
      541 ctcatagtca tgctcataat ttccgttgtc gggaacggcg cggaggaaaa aacgctccat
      601 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
      661 cagcaacaga tgcagtaaac cgatgttaac gcgcggcata tcagccagct gattcagaat
      721 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
      781 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
      841 ataaaggtgt gattaaaaaa acagcattcg ggagagtatt acgctctccc gctctgtcag
      901 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
      961 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc caacatcgcg tgcggcggta
     1021 atatcgttat cggaatcacc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     1081 tttgtatttt gacctggttt atcgcccgca aagatcaccg gattcatgtt ggtggcagga
     1141 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttggtcgg gctacgacca
     1201 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     1261 tcttttggaa tgctgaattc atcccagcca ttgttcattt tttcccagaa cacaggattt
     1321 ttcagataat cttcgctttc tggtgagaag gtttttttgc cgcgccagaa acccggactg
     1381 gaaaaaagta ccgtatcatc gatatcaaac cccaccgcca ttggcggacg tcctgcgagg
     1441 ctattttcaa tttgtgcaac cgaaacccaa tgaattggtg cctgttcagc gagtttggct
     1501 acgttagtac ccggattaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     1561 agagcgaaca ataagcaaac ggcactcaat gcctgtgtga tcttgcgcat atttttccct
     1621 aaatagtcag taagttgaaa cttattattg tgcgtttgtt gaaaaaacca tctgaccata
     1681 acgccaaccg caggggaaag gaaggattat ctgcggattt gtcacataaa aagagcatga
     1741 tcacatttgg aatacctgtg cgataaacca cacattgcca cggttattct gcttacacat
     1801 tcacacaatg gcactctttt ggttctcgca aacattgttc agggaatata ctgatgataa
     1861 atataaatgc attcaatgga tgatgaatga aaatatcgtg gaattatata tttaagaaca
     1921 aatggcgatt tcacattaca agcatttcac tttttcttat catgctcgcg gtttcaatcg
     1981 cttttttgca cttgcgtttt aataccttgt ccggtaccga taaaatgcgg cttgaaatgt
     2041 ataagtccac attatattcc accatcgagc aattttatgt tttaccctat atgctctcaa
     2101 cagaccatat catccgtcag gcggtaatta cgcctgacga tatgacgtcc agcgaactca
     2161 atcaacgaat tgcacatttc aatactcaac tcaaaaccgc agcaatattt attctggata
     2221 cccaaggtaa ggccatcgct tctagtaact ggcaggaccc cggcagctat gtagggcaaa
     2281 attatagcta tcgcccctat tataaacacg ccatgtctgg cttaaatgga cgcttttacg
     2341 gtattggtag cactacgaat acaccgggat tcttcctctc tacaagtata aaagataaag
     2401 gaaaaattgt cggtgttgta gtagtaaaaa taagtcttaa tgaaattgaa aaagcatggg
     2461 ccgaaggtcc tgaaaatatt atcgtgaatg atgaacatgg gattatattt ttaagttcaa
     2521 aatcgccatg gcgaatgcga acactgcaac cgttacctgt tcaggcaaaa caaaaactac
     2581 aatctacccg ccaatatagt ctcgacaatc ttttaccggc ggattattat ccctgttata
     2641 ccgtgagcaa ttttactttc ctgaaagata aaaaagaaca actctgttta ttcccgcaat
     2701 attatacgca acaaatagcc attccagaat ttaactggaa aatgacaatt atggtcccct
     2761 tagataacct gtactggtca tgggctattt cgttagtcat tacactaatt atttacctgc
     2821 tgtttttgtt atttattaaa tactggagaa tgcgatctca tgcacaacaa ttattaacac
     2881 tggcgaatga aacattagaa aaacaggtta aagagcgtac atctgccctg gaattgatca
     2941 atcaaaaatt aatacaggag ataaaagagc gcagtcaggc tgaacaagta ttacaaatta
     3001 cgcgtagtga actggcagag tccagcaaac tggcggcgct tggacagatg gcaaccgaaa
     3061 ttgcccatga acaaaatcaa ccgttagccg ccattcacgc acttactgat aacgcgcgta
     3121 ctatgctaaa aaaagagatg tatccacagg ttgaacagaa tctgaaacac attatttccg
     3181 tgattgagcg gatgacgcag ctcatttccg aacttaaagc atttgcctcg cgccatcgcg
     3241 tacctaaagg ttctgccgat gtcatcaaag tgatgtatag cgccgtggcg ttacttaatc
     3301 acagcatgga gaaaaataac attgagcgac gaataaaagc cccatccatg ccgttatttg
     3361 tcaattgcga tgaactcggt cttgaacaga tattcagtaa tttaattagc aacgccttag
     3421 attctatgga aggtagctct tacaaacgac tggatatcgc cattcgccag gcaaataaca
     3481 aagttattat taccattaaa gacagcggtg gcggttttgc acctgaagtt gtcgatcgca
     3541 tatttgaacc attttttacc actaaacgta gaggaatggg gttgggactg gcaatagtca
     3601 gcgaaattgt ccgaaattcg aacggcgcac tccacgccag taatcatcct gaaggcggcg
     3661 cagtaatgac attaacctgg cctgaatggg gagaaaaaca tgaataataa attcagtgaa
     3721 attactatag tttatattga ggatagtgac gatgtacgtt ttgcctgtga acaaacgctc
     3781 acgctggctg gttatcgcgt tatttcctgt tgcgatgcag aacacagtat tccccttatt
     3841 caaagtcagg ccaacattat tatactgacc gatgtcaggt tacctggaat atcaggacta
     3901 gagctcctca gttacatcaa cgaaatggat agcaaaattc cggttatttt aattaccgga
     3961 cacggtgacg tggaaatggc ggtggatgcc atgcgtaatg gtgcattcga tttcattgag
     4021 aaaccaagta gctcagataa attattgagt attattgcca gagcggtaga aaaacggcga
     4081 ttggttttag aaaatcagca attactggcg aatctgcaac aggaaaatgg cccggtgctg
     4141 attggtcgta gtccgcagat gcagcaactg cgtaaaatga tccttaatgt cgcagatacc
     4201 ggtgccgatg tccttattta tggtgaaaca ggttgtggta aagaagtcgt cgccagaatg
     4261 ctgcatcact ggagtacccg ccgtcagggg cagtttgttg ccttaaactg tgcaggctta
     4321 ccagaaacgt tgtttgaaag cgaaatattt ggtcatgagg cgggcgcatt taccggtgcg
     4381 gttaaaaagc gtattggaaa aattgaacat gcaaatggtg gtacgttatt tctcgatgaa
     4441 atagagggaa tgccatcagg aatgcaggta aaactgctac gtgtattgca ggaaagaacc
     4501 attgaacgat taggtgccaa tcaattaatt ccggtgaatt gccgtgttat tgcagcgacc
     4561 aaggaagatt tactgcgtcg cagcgaagaa catctgtttc gtctcgatct ctattatcgt
     4621 ctgaatgtgg ttagcctgaa tattccacct ctacgccagc gacgagaaga tatccctgaa
     4681 ctgttttact ggttcgccag tcaggcggca caaaaatata atcgtcctct gcctgatatt
     4741 tctcccatgt tactggcctg gttgcagtca caaagttggc ctggcaatgt acgtgaactt
     4801 aaacacaatg cagagcgttt tgttctcggt ttactgaccc accaccagcc agtgccaatg
     4861 actcagcaag aagagagtgg tcttaccgcc tgcattgatg ctttcgagaa aaagttaatc
     4921 gaagatatgc tgcgacaaac ggaaggccag gtcagtttga cagcgcggct attacagctg
     4981 ccgcgcaaga ctttatatga caaactcaac aaacatcaga ttcaaccgca agtttatcga
     5041 ccagaatcat ccagctaatg ctgattcctg aggatgtaac cccaaatcga tacctttgcg
     5101 ggcgtaatgt tcaactaccc aggaccagaa tgataccggg atcgtaatca tcttcgcatt
     5161 gcgttcccgg ttggcagctt ctggcatacc ggggtaaatc accggattgc cgccaggttg
     5221 aggctgcgta gctaacagat aatccgcaaa gaactgccct ttctcccctg ccagatcggt
     5281 gaattgatca ggatcgataa tcaacgcaaa aagatgatta tgcgcagatc catccgtctg
     5341 atgatcttcg gcgatcgtgt caccaccaga taagatcccc gccatcattt caatcatcag
     5401 tgccaggcca aatcctttat gctcaccaaa tggcagtaac gcccctggag gattgctgaa
     5461 taacacccca ggatcattgc ttggattccc ctgtgcatca atcaattgtc ccggcgcggc
     5521 ctgcttcccg gtggacgaca ttacccgtgc tttatttagc gccatgctac tggtggcaaa
     5581 atcaaccaga atcggcggac gcccatcact gaacggccag gcaaagcaga aagggttagt
     5641 ggtcatacgc ggttccgcac cgccccacgg ggcgaccagt gcaggacgcc cggtgacgtt
     5701 actcatggcc agcatcgcca ggccttgatc agcaaccatt tgcccgtaat gccccatacg
     5761 ccctaaatga tgcgcatggc acaacgttaa caggcaaaaa ccgcggtgtt ttgtttgttc
     5821 gatggcagca tttaccgcca cttttccggc gtgttgacca aagccattgt gggcatgaaa
     5881 acgctgtaaa ttaccttcac ttgttaagca ttccgggcgg gcattcgcgg ttacatcgcc
     5941 acgagcgata ttttccagat atttcggtag cagcgtgacg ccgtggctgg caaagccata
     6001 acgttccgct tcaatcatat gttccgccac atcctgagcg atatcggcat cacagccatt
     6061 aagttccagc agcccggcag ctaaccaacg cactttttca acagcaattt gtaatgacat
     6121 cgttggtttc ctcacattag acctaaaatt ttccaccagg tggcggcaaa cagtagcaac
     6181 aacaggtagc cgattaccgt caggtacagt cccacacggg taaactggcg tggcgtgaag
     6241 gtatccgtac ccatacacac catattttgt ggcgcgttaa tcggcaggat gaagccaaaa
     6301 ctgacactga aggcgagcag aatggtcatg ccgaccgggt taacgccgag ttctggcggc
     6361 agactgctta acaggctaat caggatgggc agcaatgcgg cagttagcgc agtggcactg
     6421 gcaaacccca ggtgaatgat tatcaagaaa gcggcgagaa tcgcgaagat ggcaagtgat
     6481 ggtaaaccgt caagaccaaa acctttcacc acatagttcg ccatccatga agctgcctgt
     6541 gtatcaagca acgtggaccc caggctgata ccaataccaa acatcagcaa cgtgccccac
     6601 tgcacacgtt tttcgacctc tttccagctc attacaccga tgcctggcag caacataatc
     6661 gccagtccgg caagggtgac agaggtggta tcaatactgt gtaattttcc gccagtagac
     6721 caaaagagca gtaacaacag cgaaataccg atcaagcgtt tttctttacc ggtggttggc
     6781 cctaactctg cgagtgcttt tttaatggcc tcgctccccc cttcaacagc ttccgtttct
     6841 ggcggcaaca atttacgtgc gaggaaatag aggatcaccg acatggtgag gctccagggc
     6901 gcgcccgcta acagccagtc aagccagctg acagaatgcc cggcgccgaa agttttattg
     6961 ataaaaccga tagaaagcag gttttgcgcc gacgaagttt gaataccaac gttccagata
     7021 ctggtcgcct gggcgataac aatcatcatt gacgccgcta aacgtgaatg tttatccacc
     7081 ttaaatgccg caatgacccc catcataatc ggcaccacgc aagccgtacg ggcagtggca
     7141 ctgggtacga ccaggctcag gacgatggtc accacaatgg caccgataat aatgctgcgg
     7201 ctactggcac ctattttcga catagtaaac agcgcaatac gtttatccag cccggtaatg
     7261 gtcattgccg ccgcgataaa cattgctgct gccaccagcg ccagagcaga attagaaaaa
     7321 cccgacagag tcattttcag cgctttcgcg gtgccaagga tcgtgtccgg gtgattcata
     7381 tccggggcaa aaccaaccat aaagataatc agtgcggaaa taacaattgc actggcggtg
     7441 taatccatcg cttcgctgat ccagacaata atggcaaaca ccagaatggc gatcatattt
     7501 tgtccggcga cgggcaaggt atcgcccaga ggcagaagag taataataat tccggcaatg
     7561 atcgccaacc acaggccggg attagggata attcgacgct tttgtggtgc gccatgtgat
     7621 attgatgaca tattcacctc attgtgaaaa ataaaaaacc tgcctggcta ttgcaggagc
     7681 tatgccatct ttaatttttt aataatgctt tgttatttaa tgattttatt atttattctt
     7741 tttattttgt gcgcggtttt ccgcacagta aataaaaggt gattctctta ccacacagat
     7801 aaaaaaaggg agcgatatgc tcccttgata ttcatttatg gattacttaa taacattcca
     7861 taacgtttcg ccaagcaacg ccgggtttct tgtccatgcc atgcctgctt tatcgaacgc
     7921 acggaattta tcctctgcac tgccgctacc gccagaaata atggctccgg cgtgtcccat
     7981 gcgttttcct tttggtgcag taacaccggc aatataacct acgaccggtt tattcatatc
     8041 atggcgaata aattccgcgg cccgttcttc cgcattacca ccaatttcac cgatcataat
     8101 taccgcgtct gtttgcggat cattttcgaa taaacgcagc gcatcaataa agttagtccc
     8161 cggaaccgga tcgccgccaa taccaataca ggtggattgc ccaagtccca gcgccgtggt
     8221 ttgcgctaca gcttcgtagg ttaatgtacc ggagcgggaa acaatcccaa tacgccctgg
     8281 gcggtggatt tcaccaggca taatcccgat tttgcactca cccggcgtga taactcctgg
     8341 gcagtttggc ccaatcagcc gcacatccgg gtggcagtca agcagccgac gaactttcac
     8401 catatccagt gtcggtacac cttcagtgat caccacaacc aatttgacgc cagcttcaat
     8461 agcctcaaca acagaatcca gcacaaacgg tgcaggcaca taaatcacgc tggcatcggc
     8521 ctcagtttgt cgcatcgcat ctttcattga atcaaaaacc gggcgatcca gatgccgttg
     8581 cccgccctta ccaggcgtca ctccacccac aatctgcgtg ccatacgcaa tagcctgctc
     8641 tgtatgaaat gtgccgtttt tgccggtaat gccctgcacc agtacccgcg tgtgtttgtt
     8701 gatcaaaacg ctcatcttaa cctcctgtta attcagtaac gcaatgatgc gtttcgcggc
     8761 atcatccaga gaatcgacgg cttccaccgt cagaccactt tctgccagta accgctgccc
     8821 ttcggcggcg ttattaccgg agagacgcac cacgacaggc agggtgatgc tggcctcatt
     8881 cagagcatga ataatggctc tggcgatcat gtcgcaacgg acaatcccac caaaaatatt
     8941 aaccagaatg gctttcactt tgctgtcgga aacaatcagc cgaaaggctt cactgacgcg
     9001 ctcctgggtt gcgccaccgc caacatccag gaagtttgcg ggttgttcac cgtatagctt
     9061 gatgatgtcc atggttgcca tagccagccc tgcaccattg accatgcagc cgatatttcc
     9121 gtccagcgag acataattaa gatcaagctt cgctgcctgg ctttcgcgtg gatcttcctg
     9181 ggtttcatca cgtagcacct ggagttccgg gtgacgatac agcgcgttat catccagcga
     9241 tactttcgcg tctgcacaca taaattcgcc cgtttcccgc aacaccagag ggttaatttc
     9301 cagcagggca aaatccagtt cgttaaaggc tttccacgcc tgattaacca ggcgactaaa
     9361 ggcagcaaat agtccgtgtt ccagttgcag aacagcaaac atttcgcgga tatggcaagg
     9421 ctgcacgcct gtcagtggat caatgctgac actgctgatt ttctccggtg tctcatgggc
     9481 gactttttca atttccacac caccttccgg gctgacaata aacgtgaccc gctggctttc
     9541 acgatccacc accatgccaa aatagagttc ctggcgtacc ggataaatgt tttcgcacag
     9601 taaaatactg ctgacatact gtccttctgg cccggtctga taagtcacca gttgcgatcc
     9661 caacatttgc tgtacaaaag cctgtgcttc cggcagctgc ttaagcactt ttacgccgcc
     9721 agctttaccg cgcccaccag catgtacctg cgctttaagt accgcgcctt tactcgggca
     9781 agcaatatgt tgccaggcat ctgccagttg cgaaatctgt tggatagcta tctcttttgg
     9841 acacggcatc cccatgccag ccagtaacga tttggcctga tactcatgta aattcatata
     9901 ttccctcagt ggtgtgtgtt atccgtgtaa tgctttatta ttcaaggcca ttgctgcttc
     9961 gtggatgact tcgcttaatg tcggatgcgc atggatcgca caggcaatat cctcgccaga
    10021 ggcgctaaaa gtcatcgcca gcgcaatttc attaatcagc tccgatgcct gcgggccaac
    10081 aatcgctccg ccaagtacgc ggtcagtgtg tttatcactg tataacgtgc aacgcccacc
    10141 ttcctggcct aatgctaagg cgcgaccgtt accggcaaac agagaatttc ctttgttaaa
    10201 gactctgcca gcggctttca gtgaggcctc gttttcccct acccacgcaa cttccggttg
    10261 ggtgtaaatc accgaaggga tcagcgcaaa attaatcggc tctaccacca gtccggcaat
    10321 ttgatcagcc accactacac cttcagccat cgctttatgc gccagcatcg gccctcgcac
    10381 gacatcacca attgcccaca gcccggcttt cccggtacgg cataagttat cgacggcaat
    10441 accgccacgg ttatcggctt caagaccgag ttgcacgaga tcgacaccag aaagtcgcgg
    10501 tacacgtcca atggcaagaa ttaatttatc gaagcggctt tcttctcgct tctcaccctg
    10561 ccgccagcgc acatgcacac cgtcatcccg ttgttcaata gcctcaatct ctactgccag
    10621 ttgcattttc atcccactgg caatcattgc tttacggact tcgttcgaca ggcgagcttc
    10681 cagtgcaggc aagaacgttg gcgccatctc cagcaacgtg acgtctgaac caacccgatt
    10741 ccacacagac cccagttcaa ggccaatcac gccagcgccg atcacaccga gacgcggcgg
    10801 cacttcgctc aatgccaggg cgccacgatt gtcgaggatc tgttgattat caatggtaac
    10861 gccaggcagc tgacgcggct gagaacctgt tgcaatcact acattacggg cgtgaaggtg
    10921 ttgatcgttt acacgtaatt gccaaatctc atcctgagca cattctaacg ttgcgaggcc
    10981 gcacaggtgc ttcactttat ttttcttaaa cagcaagctg atgcccattg tcaggcggct
    11041 tactatggcg tctttacgtt gaatcatcgc agctgcattg aaagatacgc cgtcaacatt
    11101 aacgccatga atgctcgcct catgctgcac ttgcgcgtac agttcggaag attgcagcaa
    11161 cgattttgac ggaatacagc cgacattgag acacgtaccg cccggcgaag gttcgccctg
    11221 tgcattaaca ccatcatcaa tacatactac ggaaagccca ttctgcgcag cccgcagtgc
    11281 cgctacgtaa ccacctggcc ccccccccat aactgccaca tcaaaaattg tactcatcgg
    11341 cgccctcctt aaagatccag caacagttgt tccggtgatt ccagtagctc acgtattgcc
    11401 accaatgtat gcacggcttc ctgaccatcg ataatgcgat ggtcatagct gagagcaagg
    11461 tacatcatgg gacgaataac aacctgaccg ttttctgcga ccgggcgcgg cgtgatggca
    11521 tgcatcccaa gaatggccga ctgcggcggg ttaatgattg gcgttgacat catcgaacca
    11581 aaagtaccgc cgttggtgat agagaacgtg ccgccctgta acgcctccag cggcaatttg
    11641 ccattgcgtg cctgtgtggc gtattcggca atttgccgtt caatttccac caaagagagt
    11701 gattgcgcat tgcgtaacac aggcactacc aggccgcggt tgctgctcac tgcaatgccg
    11761 atatcgcaat aatcacgcca gataatctcg ttgccatcga cgctggcatt cactacaggg
    11821 aagcgctcca gcgcccgggt aacggcctta acaaagaagg acataaagcc cagcttcacg
    11881 ccgtgcttct cggcaaaacg atctttccag cgggcgcgta agtccatcac gctctgcatg
    11941 tttacttcgt tgaatgtggt aagaatggcg ttattttgtt gggaagccag cagacgctca
    12001 gcaattcgct gacgtaagcg cgacatcggc tcacgacgtt cctgacgagc acccggtgtc
    12061 aagggtttag ctggcgcgat ctccgccacc cgttcaggct gaatggctgg tgcaggagta
    12121 acgcgctgca catcctcttt caggatacgt ccatttctcc cgctccccgc aacgtcagca
    12181 agttcgacac cactacgctg tgcctccagc cgtgcggaag gcatcgccag ggtttcggtg
    12241 actggtgtga cggtttcttc tatgacagct tgcggcttaa gatgcgccaa cagttgcgcg
    12301 gacgtgaccg tgctaccttc gctgacgata atattgctta acacgccatc gtgcggggcc
    12361 ggtatttcca gaatgacttt atcggtttcc agctcggcaa tcacatcatc gcgttttacg
    12421 tgctcgcctt cttgtttaca ccaggttgtc agcgtgcctt ccgtgacaga ttccggtaat
    12481 acagggacag taatttcgat cataaaatgt tctccttatt atttttgtgc gcctttcatg
    12541 gctgaatatc tgcaaacgca gcagcaagaa attcgtcgat ttgttgttta tgtacacgcc
    12601 cataccctgt cgctggtgct gccgctgccg ggcggcctgc atactgccag taaggagtgt
    12661 taattttcag tgccgccagc tcatgacgaa tctgccgcca cgcgccctgg ttttctggct
    12721 cttcctgcaa ccaaatccat tcgcagcagt tcggccaact cgccagaaca tcattcagtt
    12781 ctgcgaccgg gaacgggtag agttgctcca ggcgaacaat cgctacctcg tcttcatgct
    12841 cacgctgctt acgggcttcc agtacgtcgt aatagacctg gccgctacac aaaataacgc
    12901 gcttaacttt ctgggactga tgcaattgcg gatcggtgat taccggttta taagcgtcat
    12961 cagtgaattc acaaagctcg ctcatcgcgc ctttaaaccg aagtaaacgc ttactcatca
    13021 ttatcactaa cggtttgcgc attgggcgta atgcctgacc gcgcaacaaa tggaacattt
    13081 gtgctgactc actgggcatc accacctgca tgttgttttc tgcgcacagt tgcagccagc
    13141 gctcaggacg tgcggaagag tgctccggcc cttgtccgtc atagccatgc ggcagaagaa
    13201 tggttaaacc gctatagcga tcccacttgg tttcgccaga cgagataaat tggtcaatag
    13261 caacctgagc gccgttggca aagtcaccaa actgtgcttc ccaaataacc agttgctgag
    13321 gcgcagacgt tgaatagcca tattcaaagg ctaagagcgc ttcttcatta agcaccgaat
    13381 cataaacatc gaaagatgcc tgaccagcgc ggatatgctg taacggcaga taacgacggg
    13441 cttccgtttg atgatgtacc actgcatggc gatgactgaa cgtgccacgc cctgagtctt
    13501 caccagaaag acgcacacca acacccgcat ccacaagcga tgcataggcc agcatttcag
    13561 ccattcccca gtctactggc tgagtacctg cggccatatc ctggcgtaac gccagttgcc
    13621 gtttaatggt aggatgtgcg accacatcgg gtggcagtgt agaaatgatt tcaccatacg
    13681 ctgccagctt ttgccgaggc aatgccgtag ataccggggc gctccagtga gggttggtca
    13741 acccatacca gttagcgcta aaagagtgaa ttgcaggttt gagaggctgc ggttcacgtt
    13801 tctggcagct atccagccag tcgcgatagc gtgccgtcat ctcatcttgt tgcgcctgag
    13861 ttaacaggcc gcggcgggca agagattcac cataacgggc gagcgtacca gggtgagcat
    13921 ccactgcctg atacatttgt ggctgggtaa gacgtggctc atcgctttcg ttgtgtccgt
    13981 gcttacggaa gcagcaaata tcgatgatga tgtcacgccg gaaggtgtct cgccattcgc
    14041 acgccagttc cattacctgg caaacagcgt caacgtcatc gccattaacg tggataattg
    14101 gggccgctac cattttggca atatcggtgc agtaccgtga agaacgcata tcctgcaagc
    14161 gggaagtggt gaagccaatc tgattgttaa tcaccagatg tagcgtaccg ccagtgccgt
    14221 agccctgagt ctgcgataag ttaaacgtgg tttggttgac gccgaggccg cccaacgcgg
    14281 aatccccatg aataagtacg cccaccactt ttgcctgacc atcctcacca cggcgttcct
    14341 gcctggctcg cacctggccc agcaccaccg gattcacgat ctccagatgt gaggggttat
    14401 acgccagcgc cacatgcagc gaaccggcag gtgtttccag attgctggaa taccccatgt
    14461 ggtatttcac atcgcctgaa ccactgccga tcgtctgctt gccttcaaac tcagcaaaaa
    14521 gctgtgccgg gtctttattc aacagattaa ccagaacatt aagtcttccc cggtgcgcca
    14581 tgccaatgac catttcctcc acaccttgcg cacgcaggcg ttttgtcagc gtatcgagcg
    14641 ccgggattgc actttcgccc ccttccagag aaaaccgttt ctgcccaaca taacgggtat
    14701 gcagataacg ctctaacgtc tctgccgcca tcagtttttc aaaacgagca atacaggttt
    14761 gggcatcagc ttgtggcgca ttgctgctct caatccgtga taacaaccag ttgatttctt
    14821 cacgattttc cagatgagca agttcataag cctggctact ggcccaggct tgctccagca
    14881 ggttaagcag ttgcttaagt ggcatttgcg tggtgtgtgc gccaaaggtg acgctaaact
    14941 cctgcaacaa gtcttcttcg cttaatcccc aaaaaccagg ctggagggag ggaacgtcag
    15001 ccgggggatt caaaccaagt ggatcaagct ttgcgcgcag atgcccctgc gtgcgccagg
    15061 cattgatcag ttgtattacc gcagcctgtt tttttaaggt cgcaccgctg atattgttat
    15121 caccggatac gctttggaca gtcagttcag gtgaaagaaa aaaacgccgc cagtcttccg
    15181 ggagttgttc cggcgtacgc aaccaggtct ggtagtactc ttcaagccag gtcgcgttat
    15241 ctccactaag cagcgtgccg ggcgaggtca tgttctccat gtttttctcc tgatggtcat
    15301 agacagctgc attactgcac aatccgtacc aacagttaaa aacactaatt ttcaaaaact
    15361 tatagaaaag agatgatgta aaagtgggcg aaaaaaggga caaaatagta gggagtggct
    15421 ggagtcagga acaggcggag agatccgcct gttgattaaa atcacatcac cgcagcaaac
    15481 gcctttgcca cgcgatgcac attttgcgtg ttaagcccgg cgacacacat gcgaccgctg
    15541 gcgatgagat agacaccaaa ttcttcacgt agttggtcaa cctgagcggc acttaaaccg
    15601 gtataactga acatgccgcg ctgattaagc agataatcga aattgcgttc tggcatctct
    15661 gtgctcaata ccttcaccag ttcctgacgc attgccagaa tgcgagttcg catctcttct
    15721 acttccacca gccagctggc tttcaatgcc tcgtcattca gcaccgcagc caccacctgc
    15781 gcaccaaaat tcggcgggct ggagtagttg cggcgaacag ttgctttcaa ttgccccagt
    15841 acgcggcctg cggcttcggc atcttcacac agaacagaaa gtccgccgac gcgctcgccg
    15901 taaagggaga aaattttcga gaacgaattg ctcaccagag cgggtaatcc agcgctggca
    15961 atggcgcgaa tggcgtaggc atcctcttcc ataccggcac caaatccttg ataggcaata
    16021 tcaagaaatg ggataagctc acgggctttg agaatttcaa tcaccgcatc ccactgctca
    16081 ttagtgagat cggcacccgt tgggttgtgg caacatggat gcagcaacac aatactgcgg
    16141 gcaggtaatg ttttcagcgt cgccaacagg tcattaaagc gcacgccgtt agtcgcttcg
    16201 tcataccagg ggtaagtact tacttcgaat ccagccccgg cgaatattgc tacgtggttt
    16261 tcccaggtag gatcgctgac ccagacgctt gattccggga agtagcgttt caggaaatct
    16321 gcgcccactt tcaatgcccc tgagccgcca agggtttgaa tggttgctac gcgctgttgt
    16381 tgcagtaccg gatggtccgc accaaacagc agcggcgcaa tggcatggcg atagctgtta
    16441 agcccttcca tcggtaaata aagcgaagca ccatgaggct gcgcattcag gcgcgcttcc
    16501 gcatccgcca cggctttcag ttgtggaata attccgtctt cgttgtagta cagaccgata
    16561 cttaaattca ctttgtcgct gcgagggtct tctttaaaac gctccataag cgtaagaatc
    16621 gggtcgccag cgtaggcgtc aactttttga aacacgcgat ggttctccag gtttacgggc
    16681 aggtggttaa accacaataa accggaagaa ggcgaagatc gagaggatgt tcaaagtcag
    16741 tcgggaagtt ggcgaagaat ttgctccact cgctcttgca gtggccccga aaccgaaaga
    16801 taaggaatgt tcctgcgcga caattcctgt tcataccaca cctgttgccg tgcgcgaaat
    16861 acctctcctt gccgggtgcc atcctgcacg aagggaaatt ctgcgccgca aagcactacc
    16921 agagagtatt cccgctccgc cagttggtga agttcttgcg gagcatgacc atattgatca
    16981 agggcataga ataaggtcgt gagtggcgac gtgtcacata taagatagtg attagcttct
    17041 gcttgttgtt cccggcgtac ctgttcgcag gcgatatgca acaaatcatc cgcagttaag
    17101 ataccgtttt tttcttccca atagtcgcgg ccaaattcag cgacatacac cgtatcaagc
    17161 acgcccgcca gcgcttttga aagcgtgctt ttaccggtag attcgccacc aagcagacag
    17221 atacggcgaa cgaaactgta ataaacatca ttcgccaaca tgtagcgata gcggtgaaca
    17281 tcggaacgaa taagcgttcc cgaaggcgct tcatcgccaa ctgggcgcgc cattcgaacg
    17341 tgttcaacgg gttgggcaaa acgccgcgcc agcacgttag caaagccatc gccatagtct
    17401 tctgcagtaa atacagcgtg tgggcggcaa cgtaatatct gcaagcatag cgtcgccaca
    17461 taatgacggt ggatgtcggc atcagcattg ttatgcggga tagcaggtaa gttataacgc
    17521 gcaacaagct ctggcgtcag taccaaaaac gtcgcttgcg ggaagcgcac ttgcagccac
    17581 gtcaggcgct tttcaggttc acagtcaggc atttccggca cggaataact gatgatgaac
    17641 agttcttcac attgcgccag tgcggtattg atcaactttt catgaccaca atgcaaaggc
    17701 gcgaatttac caaccaccag acccgttgca aaaggcttca tgatgtttgc aactctttgc
    17761 gccattgata caaaccatac caggcgttaa tccagaacag gaaatataag ccagcggtca
    17821 ggtttaaacc gcgcgtcata tacagtggca ccgccagggt attcaccgct aaccagacgt
    17881 accagttttc gatacgtctt cccatcaaca tgaattgtgc cagaacgctg aaggtcaaaa
    17941 tcaacgaatc cagccagggt gcccaagcat tggtaaaggt gtggagtaaa aagccgtaac
    18001 cgcctgccac gacgacagca cagagcaaca aaaggaaaaa gtgactggct tgcgttcggc
    18061 gcaccggcaa gatgtcgcca ccctgacctt tcagccagtg gatccagcca gtgatgctgg
    18121 tcacgatgaa gaataactgg agcgtaacgt cggcatagag ttgcacggac caaaaaaccc
    18181 agccgtacaa tatgctgcca attatgccga tccaccatgt atgcacattg tttctggcag
    18241 ccagccagac ggaaaccgcg tatgccagac acgcggcgat ttctgaataa gacattgtcc
    18301 ctccctggag acgaatatta atccacgtat ttcatcgcga ctcttgaagt caggcgcgta
    18361 ataagttcgt aagcacttac cttcgtcatt tcagcgatac gttctacggg caaaccttcg
    18421 ccccataaaa tgaccggggc cccggcttta tcctgcgcct gtggacctaa gtctacgcag
    18481 atcatatcca tcgccacgcg cccgacaatc ggcacttcgc gaccgttcac cagcactggc
    18541 gtaccggacg gcgcggcgcg cggataacca tcgccatagc ccatcgcgac tacgccaaga
    18601 cgagtatcac gttcgcttac ccaggttcca ccataaccga caggctctcc ggctttatgc
    18661 tcacgcacgg caatcaggct ggaggttagc gacatcactg gctgacagcc aaaatcggca
    18721 ccggtggagc gatcttccag cggagatacg ccgtaaagaa tgatgcccgg gcgcacccag
    18781 tcaaaatgcg actgtggcca gagtaaaatg ccacccgacg cggcaatgga acgttgacca
    18841 ggcttgcctt cgcaaaaggt attaaagata gcgagttgct tctcggttgc gccacatttt
    18901 ggttcatccg cgcgcgcaaa atggctgacg atattcaccg gctgacgaac gtttttgcac
    18961 tgggtcagac gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata
    19021 ccggtatcga gtttcatcca gacggtaacc ggctcgtcca ggttagcctc ttccagcgca
    19081 gcaagctgtt cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggtaga
    19141 tctctggcat caaaaaagcc ttcgagtaaa agtacaggtt tggtgattcc ccccgcacgc
    19201 agtcgcagag cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc
    19261 gcggtctcaa gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg
    19321 gcaggtgcca gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca
    19381 acagttgccg cttgcatttg tgttccttga taagtgtttg ctttaattac ctaattcata
    19441 aaataattat tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg
    19501 accgttaaag gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat
    19561 ttccgcgatg ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat
    19621 gatcaagtcc gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccgggcg
    19681 tttgtcggca cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt
    19741 cagttctttc gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt
    19801 atcggaaagc gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc
    19861 gccgtgttca cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga
    19921 ggaatcatcg atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg
    19981 tgcccaatct tcatcatcga gctgcccggt acggatttta gtctggtcaa cacgcgacag
    20041 cgacgccaga gaacgcatca taatctgttc tgatggcatc tccagcgaga agataagtac
    20101 cggtttatcc tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg tcgttttacc
    20161 catcgacgga cgcgcggcaa cgatgatcaa atccgacggc tgcaagccag cggttttttt
    20221 gttgagatcg tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct gctgaaacaa
    20281 ctgctcaata cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc
    20341 tttgtttgca cgactttcgg caattttgaa gacgcgggat tcagcaaggt ccagcagatc
    20401 ttcactggta cgcccttgtg gatcaaaacc ggcttcggca atctcattcg caacggagat
    20461 catctcacgg acaacggcac gttcgcgcac gatatccgca taggcactga tgttcgccgc
    20521 acttggcgta ttttttgaca gctctgccag ataagcaaaa ccaccgacgc tatcgagttg
    20581 cccctggcgt tccagcgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg
    20641 caaacgcgcc atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat cgtctgccac
    20701 cacacgctcg gctacatcat cccagcgttc gttatccagc attaaaccgc ccaacaccga
    20761 ctgctccgct tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc
    20821 gcggttatca gtctgttgtt tgttgaaagg tctattgcct gccataacga atggtgttac
    20881 ctgctgttga atgggggaca agagagtata gcgagaaaca gtttctcgct acagcattta
    20941 acgcccccgc tttccggtgg aagcgcgaat ttgcagggtc gggcgataga tgtaatggct
    21001 gagcggtgcg tctggttgcg caatacgccg taaaatttgc tgcgccgcct gtttccccag
    21061 agccaatggt tctaccgcaa ttgtggttaa cggcgggtta ctgtaagcac aagccgccac
    21121 gccatcatta ccaatcaacg aaatatcttc cccggcaaat aatcctcttt cgcctagtgc
    21181 cgactccgcg ccaaaagcca ttaaatcgtt ataacaaatc accgctgtgg gtggcgaagg
    21241 catatccaac agctgttgca tcaactggta gccgttggcc tgagtcggtt cagcacgcag
    21301 cgaaaaagca tcagtactcc acggctgtaa accatggtga ttcatcgcct caaggtatcc
    21361 ctgataacgc atatggtgcg taagaccacc aataaacacg atatctctgt gcccttgttg
    21421 aatgagatat tccgtcgctt cccgtacacc tgcgtggctg tccacgccaa cgtaatcgac
    21481 gggcaattgt tgccaatcca gagggcgcat cgtgattaat aagggtgtgg aactgttggc
    21541 ataacgctgt aaatcggctt ctgaggtact gttcactgga cagaggacaa tgcccgcgac
    21601 gtggtgttcc agcaaggtat caagcacctg tttttgccgg tcactacgct gggaggtatt
    21661 ggacatcacg gtgataaaac cgtgctccgc cagcgtcatc tcaagaccca tagtaaattc
    21721 agcgaagaaa ggatttatca aatcgtcaat gactacgccg atagttagcg atttttgcga
    21781 acgcagattg gcagcaaaac ggttatagac atagcctaat tgttcaatgg cttgctggac
    21841 tttttgttgc gtctcttttt tgataagtga actgtcgttg agaaccagcg atacagtcga
    21901 tttcgaaacg ccagccgcac gcgcaacatc ggttaatgtt acccgctttc ttcctgtatc
    21961 cgacttcgcc atgcctgctc caaaccccca taagatcaag gcaattactt tatcataatc
    22021 tccttttact catgcgatcc gttaacgcaa gggatcgcag gagatcgtcg tttcgatcac
    22081 agattcgcac ttcccttgtt gtgtttttta caccctaatc gtacaaatta cgcataaatt
    22141 agatcgttcc aattaattta tgaaaattat catattgttt taaatgtata tttttaatta
    22201 tttttatatc gaaacaagga aattattatg tgctctgtac gcacgatcac tgcaccggaa
    22261 gctgcagatt tgattgttga tggcgcagtt gtcaccgtct cctcctccag tggaatgggg
    22321 tgtccggatg cggtgctggc agcaataggt gaacgctttc aacgtgccgg acatccgcaa
    22381 aacatcacct ccatccaccc tatcgccgct ggtgatatgt atggcgtaaa aggcatcgat
    22441 catctggcgc aaccgggctt actggcaaaa gtgatcgctg gctcctaccc ctccggtcca
    22501 tcatcaatga cgcctccgtt aatctggcaa atgatcaatg acaacgcgat ccccgcctgg
    22561 aatctgcctt ccgggatctt atttgatatg caccgggaag cggcggctca tcgtcctggc
    22621 gtgctgacac aaacaggcat ggatacctat gtggatcccc tgttgcaggg cggtgcaatg
    22681 aatgaaaaag cggcgcaaca atctctggtt gaacgtgttc gatttgctaa cgacgactgg
    22741 ctgtttttcc ccagtatcgt gccgcaggtg gcgatcattc gcgctaccac tgccgatgaa
    22801 cgcggcaacc tgacttatga acatgaaggg gcttatctgg ggccactgga acaggctact
    22861 gccgttcgca ataatggtgg catcataatt gcgcaagtaa aacgacaggt tgccgccgga
    22921 agtctgaaac caaaagaggt gcgtattccg ggtgtactgg tggattacat cgtgattgcg
    22981 ccggaacaaa cacaaacgac acaaacgcaa tatgaacctg ccatttccgg cgaaatcagt
    23041 cgtccgctgt cagcctttcg ttatatggaa catggcccgg cgcgagttat tgcccagcga
    23101 gtcgcgcaag aactacaaag tggagatgcg gtaaatattg gctttggtat ttctgccaac
    23161 gtaccacgca ttttactgga acaaggtcgc cacggtgacg tcacctggct gctggaacag
    23221 ggcgcgattg gcggagttcc actgctggaa tttcagttcg gttgtgcctc taatgcagaa
    23281 gcatttttgc cctctcccca gcagttcacc tatttccagg gtggtggttt tgatctcact
    23341 ctgatgtcgt ttttacaaat cggcgcagat ggctcagtga acgtctcaca tctccccgct
    23401 cgtccacatg tcactgccgg atgcggtgga tttattgata tcacctcaca tgcgaaacgc
    23461 attattttct ctggcttttt caatgccggt gcccaactgc aactggaaga gggtcagctg
    23521 cgtatcatta aagaaggcaa agccaaaaaa ctggtacagg acgtcgccca cgtgacgttc
    23581 tccggcaaac gcgcaataag actcgggcaa caagtacagt acattactga acgctgcgtt
    23641 ctggaattaa ccccagacgg gctactggtc acagaaattg ccccaggtat tgatcctgaa
    23701 cgcgacattc tcgcccaaag cgatgcccca ctccgtctgg cagacgatct caaactaatg
    23761 aatcccctct attttcaaca aggaagtcac catgaataac ggcaccattg acctccagcg
    23821 caacggtttc attgccactt tggtgattaa tcgcccggcg aaactcaacg cattaacccc
    23881 agagatgctc gaacaactgc tggcgcattg ccacacgttg gaaactgatt cggagatccg
    23941 cgtagtactg ctgaccagtg ccagtgaaaa agcgttttgt gtcggcgcgg atattcatcg
    24001 ctggacctct ctttctgcac tcgatatgtg gcgcaactgg atccgccgcg gtcatcaggc
    24061 atttgatgct ctggcacaac tgccacagcc cgtaatcgcc ctgttgcacg gcctggctta
    24121 tggcggtggt ctggaactgg ctgctaccgc cgatatccgt atttgtagcg ccgaagcaca
    24181 aatggcacta ccagaaaccg ggctggcgac agtgcctggc tggtcaggaa cgcagcggct
    24241 gacggcactg cttgggcgct cactggtgaa agaactggta tttaccggtg aaccactgga
    24301 tgcacagcgg gcactgtcct gcgggctggt gaatcgcgtt gtcgccaggg aggcgttaca
    24361 cgacgccgga ctgcaactgg cacaacgtat cgcccagcgt gctcccgttg cagtgcagat
    24421 cgccaagcag gttattgatg ccggagaagg tattgctgtg gcctcgacgc ttgaagcact
    24481 ggctggcgct ttttccgcca cgactgaaga tgctcatgaa ggcagtcagg catttactga
    24541 gaagcgtcaa ccgcactttc aggcacgctg atcgcctctt acaaggaaag aaagataatg
    24601 aatatgacaa cctctgttcc ccaaacgcct tctttatttc ggcgaataat gacaaaactc
    24661 gcggcctttg cacctggcat ctttcttatt ggctacagca tcggaacagg cagcatcacc
    24721 actatggcta aagcaggtgc taactatggt gtctcgctca tgtgggccgt ggcgctttct
    24781 tgcattgtta cctggttttt aatccgtatt tatggtcgtt atgcttccgt cactggtgaa
    24841 cgcgcgttga ctgcatttcg tcgccatatc catcccggcg ttgcgatgtt gtttctgatc
    24901 gggttaaccg ttgccgtgag cggttccatt atgggggtaa tgggtattct ggcggatgta
    24961 atggcaacat ggtccacgac ctggtttagt ggcggtatct cagctctcgc ctgggctatc
    25021 gcgatggtca ttatgattaa cgtgatgctc tggaccaaca gcaacgccgg gttcgacaaa
    25081 atcctctccg cgctggttgg cattatgggg ctggcgtttg tgattaattt tttcattatg
    25141 atgccgccgg tcgcagatct gctggccgga tttattccgt ctgttcccga tcctgtcgct
    25201 ggcgataaca gtacgccatt tctgataatt tctggcatgg tgggtaccac cgtagcacca
    25261 gcaatgtttc ttatgcgcac gaccctggta aaaagtaatg gttggggtgt ggacaatttg
    25321 cctcatcaaa atcgtgatgc cattgtcagt gcaggaatga tgttcatcat cagtgcggcg
    25381 gtgatggctg cggctgcggg tacgttgtac caaactggca caggtttaaa taatgcctcg
    25441 caaatggtca ttattctcga acctattgct ggctcagctg ccgtggggct ctttgttatt
    25501 ggtattgttg cagccgggct ttccagccag ttcccaaatt tgctgttaac gccctggctg
    25561 ctggctgact ggcgtgatac cagcaacgaa gttagcccag gttaccgtat cctgatgacg
    25621 gttatgtctc tgctttgtct ggttgtgccg ctgttccacg cacgcccggt attggtgatg
    25681 attgccagcc aggcctttaa cgccattatt ctgccgctga ctgttttatg tatgttctgg
    25741 ctggcgaaca agccttcgtt aatgggcaaa tatcgttgct cacgctggga aaacgtcatg
    25801 tttattgcca taagcgtatt tacgctaatc atgggctata tggcaatgag cggcttaatc
    25861 gaaagtctga tgaaataggt gggacgcaat gattgacgaa acgctaacgg cgctcactga
    25921 tatcaaaatg ccccattacg caccgctggc tcaagccagc ggtagtatcg accttaacgg
    25981 taccatttta ccagagtact gctgcaacac gctggttctg ggcagtggcg cggcaggatg
    26041 gcgagctgcc gtcgagctga aacggcaaaa tgtcgatgta atggtcgcct ccagcaaagc
    26101 cttctggggc acttccgcct gttctggttc cgataaacaa acgcttcaca ccgcgaatac
    26161 gcgtgcaaat ggtgaccatt ttctgacatt aagtaacacc ctggcggcag gtggtgcgat
    26221 ggatcatgac accgcatatg ttgaggcggt cggatcggtg aacacctgtg aagtactgaa
    26281 atatctgggt ctggatctgc ctgaagatct ctttggcgca acgttgcgtt atcaaaccga
    26341 tcacgatgaa tttggccgtg ccactagctg cggtcctcga acatcgcgcc tgatggtgaa
    26401 ggtgctggcc caggaggcaa tgcgccttaa tattcctctt tgcgatcaca caacggcgat
    26461 tcatatcctg accagtggtg aaggggaaaa tcgacgagtc gtgggggtga tcgccatcga
    26521 taaagcctgt cgggataatc cctggcgaat ggtcgtgatt cgctgccagc atctggtttt
    26581 agccacgggc ggaccaggtg aactttatcg tgacagtgtt tatccggtga actgttttag
    26641 tgcgttgggc atggcactgg aagcaggtat aactctggtt aatcttacgg aaagtcagtt
    26701 tggtatcggt acaccgcgca gccagtttcc gtggaatctt tccggtactt acatgcaggc
    26761 gatgccgcgc atctattcgc aggaccactc cgggcgtcag tataattttc tggcagctta
    26821 ttaccccacg actcgtatgc tggcctccgc aatatttcgt aaaggttacc agtggccgtt
    26881 tcacgcggaa cgaatgctgg aatatggctc cagcctgctg gatgtcgcag tttatgaaga
    26941 gactcaaaaa gggcgcgatg tttttcttga ttttctacgg gaaccagaac ccgcagcgga
    27001 cggaacgtca ttcaacctgc aagaactgga tgatgatgtc atcgactatc tgcaacaaaa
    27061 tcacgccctg ttggcacaac cgctggcgag attaacacag atgaatcctc tggctattcg
    27121 tttgtatcag atgcacggtc atgatctcac agcctcaccg ctcaagttta cgctaaataa
    27181 tcaacatctt aatggcggaa ttgaagttga tatttgggga cgtacatcgc taactggttg
    27241 ttatgctgcc ggagaaaatg ccggaacaca tggtgtcacg cgcccaggtg gagcagcgct
    27301 caatgccggg caagttttcg cccgccgctg tgcattacat attgctcatc agaaaagcca
    27361 ggaccggcct ctcgagaggc aacaaattct cagtacgatt aatgaggcgc aacagtacct
    27421 gaatcagggg ctgctacgtg acgacgtgcg agaaacgata caaaatacca tgagccacca
    27481 tgccgcaatt ttgtgtcata cacaggggat caacactgcc gcgcaaaccc tggcaacact
    27541 ttgtgaagat attcgccggg aaggtattca atgcgatgaa aacagcctgg cacaatcttt
    27601 ccagtggcgg caaagtgcgc aactggcgct ggctgtactg cgttctttac aatgttatgt
    27661 cgaaaacggt ggtgggagtc gtggtgccag ggctatctat gatggcgaag ccggtattgc
    27721 accgcaaaca ccaggtgggc cactcctggc ctggcgtttt cggccagaaa atcaggatgc
    27781 caggcaatat atgatttgtg tttgtcggga agctaatgat taccaaacct ggactcgccc
    27841 gtgccgtgaa cggggcgtat taacgctaac caactttgag cgccagtggc aaaactggat
    27901 tgcggcgacg ccgtttcaat actgatcaga gatattccgg gttgtgaccg cagcccggaa
    27961 aaaatccccg cgcggcctgg cgactactgt taggcttaaa acagatatgt cgctcagtaa
    28021 aggagtttaa atggcaacgc gcatcgaatt tcacaagcac ggtggcccgg aagtacttca
    28081 ggccgtggag ttcactcctg ccgatccggc ggagaatgaa atccaggtca aaaataaagc
    28141 catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc caccatcgct
    28201 acccagcgga ttaggcaccg aagcggcagg catcgtgagt aaagtcggca gtggtgtaaa
    28261 gcatattaag gcaggcgatc gcgtagtcta tgcacaatcg gcattaggcg cttacagctc
    28321 tatgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt cttttgagca
    28381 agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca aaacctatga
    28441 aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcgttg gcttgattgc
    28501 ctgccagtgg gcaaaagccc tgggcgcgaa acttatcggc accgtgggaa ccgcgcaaaa
    28561 agcgcagagt gcgctaaaag cgggcgcatg gcaggttatt aactatcgtg aagagaatct
    28621 ggtcgagcgg ttaaaagaga tcaccggtgg caagaaagtg cgcgtggtgt acgattcagt
    28681 gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct taatggtgag
    28741 ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca atcagaaagg
    28801 ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg aggaattaac
    28861 cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg tcgatgtcgc
    28921 cgagcagcag aaatatccgc tgaaggatgc gcggcgtgcg catgagattc tggaaagccg
    28981 ggcgacgcag ggttccagcc tgttgattcc ataaaagaaa ttgggcttcc acctgggaag
    29041 ccctttcttt tatagttcgg ctgtatgtag ggtacagctc gatgaatttg tttgacacgc
    29101 aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt
    29161 gatcatccgc acaatccctt agtaacgcca gcggtcataa tgctgatatt tcggcacttt
    29221 tggtgcttta atcgccctga tcacccacac caccgcaacc gccaacagca gccacggcag
    29281 caacttaatc atcaatgcca gcataccgcc gaggaacata aaggccgtcg ccacaatcag
    29341 cgcggcgata atacccagca acgaaacacc agtgaccatc agcatgacaa aaaagccaat
    29401 tacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca
    29461 agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag
    29521 ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc accagtttga
    29581 gcgcgtgttc cagcacatta atgtctgcac ccgctttatg ggcattttca cttaaataac
    29641 gtcgccactg ccgcgcgcca ggaataccct ggaacaagcc cagcatatgc cgggtaatat
    29701 ggccgagata cgtcccctgg ctgagttcac gctcaatgta cggatacatg gcgcgcacta
    29761 ccgccaccgg atcggcatcg gtatccgagg aaccaaaaat ctctcggtct accgccgcca
    29821 gaatccccgg attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaaat
    29881 gcgctttagc ttcttccagc gacttgatac caccgttaat cgacatcgtc agatgcggaa
    29941 agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatctca cggttttctt
    30001 tcggacttaa cccagaaagc caggctttac gtgcgtggat gatgaacatc tcacactcac
    30061 ctttgccgga aacggtgttg atgaaatcgc agagaaattc atagctgtcc tgatcatcga
    30121 tgccaatacg cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc gctttcacgc
    30181 agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg ttctgcaccc
    30241 ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccgcgcgct tctgccagct
    30301 tcgcacactg tgccagcgcc gccggatcgc tacccccgag ttgcaacgct accggatgtt
    30361 cttcttcact gtacgccagg taatcacctt taccgtgaat aatcgcccct gtggtcacca
    30421 tttcggtata cagcaacgta ttgcgggaaa gcagacgcag gaaataacgg caatgtctgt
    30481 ccgtccagtc aagcatcggg gcaatcgaga aacggtaagc gggataaaca gtgtgtgatt
    30541 ctgctggcat ggcgactttt taagcatctg gtaaattggg ggcgctacta tagcataacg
    30601 aatatgcgat caggcaatgt gacagccatg acaccctgtt ctcggcgttt aagtgagaac
    30661 tatggtaaag taaggacatt cttaacccca ctgtcgaggt gcccaatgga aaagaccaca
    30721 acgcaggagt tattagcgca ggctgaaaaa atctgcgcac agcgtaatgt gcgcctgacc
    30781 ccacagcgtc tcgaagtgtt gcgcctgatg agtctgcagg atggcgctat cagcgcttat
    30841 gatctgcttg atttgctgcg cgaagctgaa ccgcaagcca agccgccaac ggtttatcgc
    30901 gcgctggagt ttctgcttga acaaggtttt gtgcataagg tggaatccac caacagttat
    30961 gtgctctgtc atctgttcga tcagcccacc catacttcag ccatgtttat ttgcgatcgc
    31021 tgcggcgcgg tgaaagaaga atgtgctgaa ggcgtggaag acatcatgca tacgctggcg
    31081 gcaaaaatgg gttttgccct gcggcataat gttattgaag cacatggatt atgttcagca
    31141 tgtgtagaag tagaagcgtg tcgtcatcct gaacagtgcc atcatgacca ctctattcag
    31201 gtgaaaaaga aacctcgcta aaagggtgta catccttgta catgtcgggc aggagggatt
    31261 aattaccagc ggtaatcatg gcgtttttcc caactatcaa cctctttttc tgcctgatct
    31321 ttctgataac cgtagcgttc ctggatttta cccactaact gatcacgttt tccttcaatg
    31381 atcgtcatat catcatcggt cagtttgccc cattgctctt tcactttacc tttaaactgt
    31441 ttccagttac cgccggcttc atctttattc ataatcaaga cctcatcgtt aggttgtgaa
    31501 tgagagtacg ttcacttttc ttctgaacgt gagattaagt gtagtcagca atttggctta
    31561 ggattattta ttcagaattt ttaaccgtca cgttgcgaca aaccaggtat cgttacgcca
    31621 gtgacgccgc cagatagccg ccagagaaag cccgcgtaac gccagaaaga cggttaacgc
    31681 cagccacaaa ccatgattcc ccagccacgg cagcgtaagg agcgtcagtg caaaacctgc
    31741 ggcggccacc gccatactgt tacgcatttc ggcggcacgc gttgcgccta taaacatgcc
    31801 gtccagcaga taacaccaga cgccgaccaa cggcaaaatc acctgccaga taagatagcg
    31861 gtcagccagc tgctgaatct gggttaacga cgtcagcaac gcaatgatgt gttcccctgc
    31921 cagcaaataa accaccgaaa acagtaacgc tacgatcccc gactggcggc acgctgcccg
    31981 ccagacatcc agcaattgac taccgtcgcg cgcaccgtat gcctgaccgg aatgtgcttc
    32041 aaccgcgtag gcaaaaccat ccagcgcata ggcggtaaag gtgagtagcg tcatcagaac
    32101 cgcgttaaca gcgataatgt cactccccag tcgcgcgcca agtacagtga tcgcgccgaa
    32161 acagagttgc agcaacagcg agcgcagcat gatatcgcgg ttaagcgcca gcaagtgacg
    32221 gaagtttcct cgccaggctg ttttcagcat ttcgccggag attccgcgta gtttgagaat
    32281 tttacgcacc attagcagac caatcagcaa tgttgcatat tccgcaataa ccgtcgccag
    32341 agccgcgccc tgcacgttca tatgcagccc catcaccagc cagacatcca gcacaatgtt
    32401 gaggatattg ccgaccacta acaaaattac tggcgcacgg gcatattgca cgccgagtaa
    32461 ccagccaagt aataccagat tcgccagcga cgccggtgcg cttaaccagc ggatttcaag
    32521 aaagcgtcgc gcctgttcca ggactgcttc actaccacca acaatatgca gcgccagatc
    32581 gataatcggc gtacgcagca gcgcaattaa cgccccggcc cccaacgcca acagcaacgg
    32641 ttgcaccagc gcacgggcta atgcctgagg atttttggca ccataagcct gtgcagtcag
    32701 cccggtggtg ctcatgcgta aaaacagcaa caacataaag agaaagctgg tcgccgttgc
    32761 accaaccgcc acgccgccta aataaaccgg actatcaaga tgaccaatta ccgccgtatc
    32821 gaccagtccc agcaacggaa cggtgatatt ggagaaaatc atgggtaagg cgagatgcca
    32881 gagtgcttta tcagatgaag tgaggaatgc catgcagaca agcctgatga agagagatga
    32941 aaaacaaacc gcgacgccag gcggcatcgc ggtctcagag atatgttaca gccagtcgcc
    33001 gttgcgaata accccaaccg ccagcccctc aatggtgaag ctctgctgac gaaggtcaac
    33061 gacaattggt ttaaactcgc tgttttctgg caacagttcg actttattgc cctgtttttt
    33121 caggcgttta acggtaactt cgtcatcaat acgtgcgaca acgacctgac cgttacgtac
    33181 atcctgagtt ttatgcactg ccagcaaatc gccatccata atgccgatat ctttcatcga
    33241 catcccgctg acacgcagca ggaaatcagc attcggcttg aacaaggaag gatcgacctg
    33301 ataatgacct tcaatatgct gttgcgccag aagcggttca ccggcagcca cacgacctac
    33361 cagcggcaac ccttcttcct cttcctgcaa taaacgaatc ccgcgtgatg cgccggaaac
    33421 aatttcaata acgcctttgc gtgccagcgc cttcagatgt tcttcagccg cgtttgggga
    33481 acggaacccc aaacgctgcg cgatttccgc acgcgtcggc ggcatacctg tctggctgat
    33541 gtgatcacgg atgagatcaa acacctcttg ttgcctggcc gttaacgctt tcattccgcc
    33601 ccctgggtgt atatacagtt atgctgtgag tatatacagc aaaaggcgat tttggaacca
    33661 taaactgcac aataaaccag agatttatcg aaatctggaa gggttatcca aaatgcgacc
    33721 ataacaggat gcaccaggta atcacggcga cgataatggc aatcagcact gcggcagatc
    33781 ccatatcttt agcgcgtccg gaaagctcat ggtattcaga gccaattcgg tcaaccacag
    33841 cttcgatggc gctattgaga atttccacaa tcatcaccag catcacggag ctgataagca
    33901 gcacgcgggt aatcgcgtcc acatccagcc agcaggcgat aaccaccgct aacaataccg
    33961 ctacgccttc ctgacggaat gccgcttcgt tgatccatgc agcgcgtaaa cctttccagg
    34021 aatagccagc agctttaata attcggatga atccagtggt attattggcc attgaaagaa
    34081 cctttttaca ttatgagcgt caatatcagt gtaccgttaa atccgatgat tcgggtttgc
    34141 gtaacgccgc gcgaaacatg agcggatacc acagaatttc ccatgacttt ctgctatcct
    34201 tgccgcgcat ttgcattatt aaccagaggc tttacatcgt ttatgtccgg ctggccacga
    34261 atttactaca aattactgaa tttaccatta agcatcctgg taaaaagcaa gtctattccg
    34321 gcggatcctg ccccggaact ggggctggat acctctcgtc caattatgta cgttttaccg
    34381 tacaactcga aagctgactt gctgacgttg cgcgcccagt gtctggcaca tgacctgcct
    34441 gacccgttag aaccgctgga aatcgacggc acgctactgc cgcgctatgt gttcattcac
    34501 ggcgggccgc gtgtgttcac ctattacacg ccgaaagaag agtctattaa gctgttccac
    34561 gactatctcg atttgcaccg cagcaaccca aatctggatg tgcagatggt gccagtatcg
    34621 gtgatgtttg gtcgcgcgcc ggggcgtgaa aaaggcgaag tgaacccgcc gctgcgtatg
    34681 cttaacggcg tacagaaatt tttcgctgta ctgtggctcg gtcgcgacag ttttgtgcgt
    34741 ttctcgccgt cagtttcact gcgccgtatg gcggatgaac acggcacgga taaaactatc
    34801 gctcagaaac tggcgcgcgt ggcgcgtatg cactttgccc gtcaacgtct ggctgccgtg
    34861 ggtccacgtc tgcctgctcg tcaggatctg tttaataagc tgctcgcctc ccgcgccatt
    34921 gcgaaagcgg tagaagatga agcacgcagc aaaaaaatct cccatgaaaa agcgcagcag
    34981 aacgcgattg cgctgatgga agagattgcg gcgaatttct cttacgagat gatccgcctg
    35041 actgaccgca ttctgggctt cacctggaac cgactttacc agggtatcaa cgtccataac
    35101 gccgagcgcg ttcgccagct ggcccacgac ggccatgagc tagtgtatgt gccttgccac
    35161 cgcagtcaca tggactacct gctgctttct tacgtgctgt atcaccaggg gctggtgccg
    35221 ccgcatatcg ccgccgggat caacctgaac ttctggccgg ccgggccgat tttccgccgt
    35281 ctgggggcgt tctttattcg ccgtacgttt aaaggcaata aactttattc caccgttttc
    35341 cgtgagtatc tcggcgaact gttcagccgt ggttattccg tcgagtattt cgtggaaggc
    35401 ggtcgttccc gtacggggcg tttgctggat ccgaaaaccg gtacgctatc gatgaccatt
    35461 caggcgatgc tgcgtggcgg cactcgtccg attacgctga ttccgatcta tatcggttat
    35521 gagcacgtca tggaagtggg tacttacgcc aaagaactgc gcggtgcgac gaaagagaaa
    35581 gagagcctgc cgcagatgct gcgcggttta agcaagctgc gtaatctcgg tcagggttac
    35641 gtcaacttcg gtgaaccaat gccgttgatg acctacctta accagcacgt accagactgg
    35701 cgtgaatcta tcgatcccat cgaagcggtg cgtccggcct ggttaacgcc gacggtcaat
    35761 aatattgctg ccgatctgat ggtacgcatt aacaacgcag gcgcggcgaa cgccatgaac
    35821 ctgtgctgta ctgcgcttct ggcgtcacgt cagcgctcac tcacccgcga gcagttaacc
    35881 aagcaactca actgctacct ggatctgatg cgcaacgtgc cttactccac ggactctacc
    35941 gttccttcag ccagcgccag cgagcttatc gatcacgcgc tgcaaatgaa caagtttgaa
    36001 gtcgagaaag acactatcgg cgacatcatc attctgccgc gcgagcaagc ggtgctgatg
    36061 acctactatc gcaacaacat tgcgcatatg ttggtgctgc cttcgctgat ggcggcaatt
    36121 gtcacccagc atcgccacat ctcccgcgac gtattgatgg agcacgtcaa tgtgctttac
    36181 ccaatgctga aagcggaact gttcctgcgc tgggatcgcg acgagttacc ggacgttatt
    36241 gatgcgctgg caaatgagat gcaacgtcag gggctgatta ccctgcaaga tgatgagttg
    36301 catatcaacc cggcgcattc tcgcacgcta cagctgctgg ccgcaggcgc gcgcgaaacg
    36361 ctgcaacgtt atgccatcac cttctggctg ttgagtgcca acccgtcgat caaccgcggt
    36421 acgctggaaa aagagagccg caccgtcgcg caacgtctct ccgtgctgca cggtatcaac
    36481 gcgccggaat tcttcgacaa ggcggtgttc agttctctgg tgctgacgct gcgtgatgaa
    36541 gggtatatca gcgatagcgg cgacgccgaa ccggcagaaa cgatgaaggt atatcagttg
    36601 ctggcggagt tgattacatc agacgtgcgt ttgacgattg agagtgcgac gcagggcgaa
    36661 gggtaatcag tgagaattgc cggatgcgac gcaaacgcct tattcggcct accatgatct
    36721 gcaaattcaa taaattgcga tatgttgtgt aggcctgaga agcgcagcgc atcaggcaac
    36781 ccagaagaaa gccggatgat catccggctt ttttacatca tcagaaatgc cagtaactca
    36841 ttgccagccc taaaaacagt accagaccaa catagttatt attcataaat gctttaaagc
    36901 aggcttcacg ctcgcggttg gcaatcagtt tttgttgata aacaaacagc gcgccagcta
    36961 ccagaattga ccagtaatat ccccagccta agccatttaa ctcaccgatg atcgccatca
    37021 gcgctagtac gccaatctgc aaaataccaa taatcaattt atcgtattgg ccgaacagga
    37081 ttgcggtgga tttaatgcca atcttcacat catcatcgcg gtcaaccatc gcatactgcg
    37141 tgtcgtaagc caccgcccag agaatattgg cgaggaacat taaccagcaa ctcaatggca
    37201 ccgactcact cacagcggca aaagccattg gaatcgacca gccaaacgcc gcgcccagca
    37261 ccacttgcgg tagatgggta taccgcttca taaacgggta tacccacgcc agcgccagcg
    37321 cggcaattga caacagaata gtcatcgtat tcagcgtcag caccagtaaa aatgaaatca
    37381 gtaccagcac tacaaacagc gtacgcgctt ctttctctgt caccgcgccg ctgggaagtg
    37441 gtcggttcgc cgtgcgctta acatgaccat caaacttgcg gtcagcataa tcattcacca
    37501 cgcaaccagc agcgcgcatc agccagacgc ccgcgacaaa caccgccagg atccagagct
    37561 ggggaacgcc cggtgtcgcc acccacaacg cccataatgt tggccagagc agcagtaacg
    37621 cgccaattgg cttatccgta cgcattaagc gatgaaacgc cagcagctta ttctgcgtca
    37681 gactccactc catatttttt tcctcttagt acaacggtga cgccggtaaa aacagttctg
    37741 ttagcaacag cggtttaccg cttaatcgca ggcgggaacg tcgcccccac agtccggcat
    37801 cacggcctat ctcaataaag tcccgggtta atgtcgatga tgtgaacaga tagcgtccta
    37861 acggcgtttt acctaatttt tgtaacgcca gctccggccc gcttaacgtt gacacaggaa
    37921 cgacggtacg accggcaagc cacggttcac catcggcaca taacaaaatt tcacgtaacc
    37981 agtaacgaga ctctttcggc agcagtggca gttcttcggg gatttcattc tgctcgacaa
    38041 acccttcgcg gatcatcgtc acgctcaccg tttttccctg ctgttcaaaa cgttttgtca
    38101 tggaatcttc cagcaacagc cagtcgagca gttgcggctc cagggcaggg atctctgtaa
    38161 aatagcgcag cgcacgcagt tgcgttaacg cggggtgtga catgccgaac tctccgttac
    38221 ataacgtaaa ggcattgtat cgcagaaaaa tatttacggg agatgtaaag caaattaagt
    38281 gaactaatgc gcaacaaaaa cgcaacaata gtacaggagg agaaaaaaag gtgcgccagg
    38341 agacgcacca gttgttgcaa aatcagcact gtggagcgta attacccctt gccttttaca
    38401 ctgctgataa aggtggaacg ggcagatgtc gaccccaggc gttcagcttc atcaagcagt
    38461 tttaacgcct tatcaacatc acctttcgcg acagcgtttt tgatcgcggt attaaaataa
    38521 ctttccgtgt cgttgagcat cggctcgctt ttcttcaccg gtgccggagc gggtgcagcc
    38581 acagctggtg ccgccgtgtt acctaccgta accggagctg gagcggaaga accaaatagt
    38641 ggtcctacca acacgctgga gctggagttc gttttcactt tcagtttcag caaaccatca
    38701 gtggtgtgac gggcaatcgg gtcaggaata tccggcaccg agttacctac gcctttggca
    38761 taggcttttg ccgggtcgag cagttgggtt gtctgctgga gatctttttc cgtggtgaag
    38821 accagtacat agagtttctg ttgccccagt gccggtgtca ggcgcataac gccttccaga
    38881 cgatctgcac tcatcacgcc gggttcctgg taggtgaaat aactgctggg gaagaaggct
    38941 gatggggtca tgttctgatc aagaatcagc acgttcggcg caaatacgct ggtttgtttg
    39001 ttcacttcgc tggtcagcgt cagtgtcagt tcaccaatgt ttgccgggac gctgtacgca
    39061 gcaaccggac cactgatgcc ggaaacgttc agttgttggc cgccggtcga cagttgagtc
    39121 gtctgggttt tagattgatc gaccggtgtc caggtgagtt gttgcagcgc ggcagatgga
    39181 atggctggcg cgtcgctggt gttttgcggt acgtagttaa catcggcaag gctgattcca
    39241 ggtgcgcttg ccagcaaccc tgctgataaa cagagagcga tgagactttt attcattttc
    39301 attgttttca cctcaaaatc tggagctcag cggtagccag gcaatagcgc gctaaacccg
    39361 ataatcagag gggctttcgc cccttaaagc tgttt
//
LOCUS       DAOFSV010000033        39111 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.033, whole genome shotgun sequence.
ACCESSION   DAOFSV010000033 DAOFSV010000000
VERSION     DAOFSV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.033"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(74..1468)
                     /gene="bglB"
                     /locus_tag="QBH27_001791"
     CDS             complement(74..1468)
                     /gene="bglB"
                     /locus_tag="QBH27_001791"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDQ7014113.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(1487..3361)
                     /gene="bglF"
                     /locus_tag="QBH27_001792"
     CDS             complement(1487..3361)
                     /gene="bglF"
                     /locus_tag="QBH27_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDQ7014114.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTVQINAGEPLLSIIR
                     "
     gene            complement(3494..4330)
                     /gene="bglG"
                     /locus_tag="QBH27_001793"
                     /pseudo
     CDS             complement(3494..4330)
                     /gene="bglG"
                     /locus_tag="QBH27_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
     gene            complement(4616..5341)
                     /gene="phoU"
                     /locus_tag="QBH27_001794"
     CDS             complement(4616..5341)
                     /gene="phoU"
                     /locus_tag="QBH27_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDQ7014115.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(5356..6129)
                     /gene="pstB"
                     /locus_tag="QBH27_001795"
     CDS             complement(5356..6129)
                     /gene="pstB"
                     /locus_tag="QBH27_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDQ7014116.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(6220..7110)
                     /gene="pstA"
                     /locus_tag="QBH27_001796"
     CDS             complement(6220..7110)
                     /gene="pstA"
                     /locus_tag="QBH27_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDQ7014117.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(7110..8069)
                     /gene="pstC"
                     /locus_tag="QBH27_001797"
     CDS             complement(7110..8069)
                     /gene="pstC"
                     /locus_tag="QBH27_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDQ7014118.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(8155..9195)
                     /gene="pstS"
                     /locus_tag="QBH27_001798"
     CDS             complement(8155..9195)
                     /gene="pstS"
                     /locus_tag="QBH27_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDQ7014119.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(9509..11338)
                     /gene="glmS"
                     /locus_tag="QBH27_001799"
     CDS             complement(9509..11338)
                     /gene="glmS"
                     /locus_tag="QBH27_001799"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDQ7014120.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(11500..12870)
                     /gene="glmU"
                     /locus_tag="QBH27_001800"
     CDS             complement(11500..12870)
                     /gene="glmU"
                     /locus_tag="QBH27_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDQ7014121.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(13224..13643)
                     /gene="atpC"
                     /locus_tag="QBH27_001801"
     CDS             complement(13224..13643)
                     /gene="atpC"
                     /locus_tag="QBH27_001801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDQ7014122.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(13664..15046)
                     /gene="atpD"
                     /locus_tag="QBH27_001802"
     CDS             complement(13664..15046)
                     /gene="atpD"
                     /locus_tag="QBH27_001802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDQ7014123.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(15073..15936)
                     /gene="atpG"
                     /locus_tag="QBH27_001803"
     CDS             complement(15073..15936)
                     /gene="atpG"
                     /locus_tag="QBH27_001803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDQ7014124.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(15987..17528)
                     /gene="atpA"
                     /locus_tag="QBH27_001804"
     CDS             complement(15987..17528)
                     /gene="atpA"
                     /locus_tag="QBH27_001804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDQ7014125.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(17541..18074)
                     /gene="atpH"
                     /locus_tag="QBH27_001805"
     CDS             complement(17541..18074)
                     /gene="atpH"
                     /locus_tag="QBH27_001805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDQ7014126.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(18089..18559)
                     /gene="atpF"
                     /locus_tag="QBH27_001806"
     CDS             complement(18089..18559)
                     /gene="atpF"
                     /locus_tag="QBH27_001806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDQ7014127.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(18621..18860)
                     /gene="atpE"
                     /locus_tag="QBH27_001807"
     CDS             complement(18621..18860)
                     /gene="atpE"
                     /locus_tag="QBH27_001807"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDQ7014128.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(18907..19722)
                     /gene="atpB"
                     /locus_tag="QBH27_001808"
     CDS             complement(18907..19722)
                     /gene="atpB"
                     /locus_tag="QBH27_001808"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDQ7014129.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(19731..20111)
                     /gene="atpI"
                     /locus_tag="QBH27_001809"
     CDS             complement(19731..20111)
                     /gene="atpI"
                     /locus_tag="QBH27_001809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDQ7014130.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(20728..21351)
                     /gene="rsmG"
                     /locus_tag="QBH27_001810"
     CDS             complement(20728..21351)
                     /gene="rsmG"
                     /locus_tag="QBH27_001810"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDQ7014131.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(21415..23304)
                     /gene="mnmG"
                     /locus_tag="QBH27_001811"
     CDS             complement(21415..23304)
                     /gene="mnmG"
                     /locus_tag="QBH27_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDQ7014132.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(23683..24126)
                     /gene="mioC"
                     /locus_tag="QBH27_001812"
     CDS             complement(23683..24126)
                     /gene="mioC"
                     /locus_tag="QBH27_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDQ7014133.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHNIPEDPAEEWLGSWINLLK"
     gene            complement(24216..24674)
                     /gene="asnC"
                     /locus_tag="QBH27_001813"
     CDS             complement(24216..24674)
                     /gene="asnC"
                     /locus_tag="QBH27_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDQ7014134.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            24826..25818
                     /gene="asnA"
                     /locus_tag="QBH27_001814"
     CDS             24826..25818
                     /gene="asnA"
                     /locus_tag="QBH27_001814"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDQ7014135.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSKEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(25823..27274)
                     /gene="viaA"
                     /locus_tag="QBH27_001815"
     CDS             complement(25823..27274)
                     /gene="viaA"
                     /locus_tag="QBH27_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDQ7014136.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(27268..28764)
                     /gene="ravA"
                     /locus_tag="QBH27_001816"
     CDS             complement(27268..28764)
                     /gene="ravA"
                     /locus_tag="QBH27_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDQ7014137.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            28987..30855
                     /gene="kup"
                     /locus_tag="QBH27_001817"
     CDS             28987..30855
                     /gene="kup"
                     /locus_tag="QBH27_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDQ7014138.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            31022..31441
                     /gene="rbsD"
                     /locus_tag="QBH27_001818"
     CDS             31022..31441
                     /gene="rbsD"
                     /locus_tag="QBH27_001818"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDQ7014139.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            31449..32954
                     /gene="rbsA"
                     /locus_tag="QBH27_001819"
     CDS             31449..32954
                     /gene="rbsA"
                     /locus_tag="QBH27_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDQ7014140.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            32959..33924
                     /gene="rbsC"
                     /locus_tag="QBH27_001820"
     CDS             32959..33924
                     /gene="rbsC"
                     /locus_tag="QBH27_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDQ7014141.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            33949..34839
                     /gene="rbsB"
                     /locus_tag="QBH27_001821"
     CDS             33949..34839
                     /gene="rbsB"
                     /locus_tag="QBH27_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDQ7014142.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            34965..35894
                     /gene="rbsK"
                     /locus_tag="QBH27_001822"
     CDS             34965..35894
                     /gene="rbsK"
                     /locus_tag="QBH27_001822"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDQ7014143.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            35898..36890
                     /gene="rbsR"
                     /locus_tag="QBH27_001823"
     CDS             35898..36890
                     /gene="rbsR"
                     /locus_tag="QBH27_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDQ7014144.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(36856..38283)
                     /gene="mdtD"
                     /locus_tag="QBH27_001824"
     CDS             complement(36856..38283)
                     /gene="mdtD"
                     /locus_tag="QBH27_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDQ7014145.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(38306..38998)
                     /locus_tag="QBH27_001825"
     CDS             complement(38306..38998)
                     /locus_tag="QBH27_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDQ7014146.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         9568 a        10095 c         9777 g         9671 t
ORIGIN      
        1 tggtgcaaag aaagaattaa cgcatcgcat ccagactgtt ctgaatgcga cgatatttaa
       61 ggtgctttat tggttatttt tttaatgata gcccccgagt tttgatcact tctgcatacc
      121 atccgaagct tttcttgcgc gttcttgtga ggcttccttc accattatca tcacgatcca
      181 cataaataaa gccgtagcgc ttagacattt gtgaatgaga tgcactgact aaatcaattg
      241 gcccccaact ggtgtacccc ataatatcca caccatcggc aatcgcttca tttacctgta
      301 ccaggtgatc atttaaatag gcaattcgat aatcgtcctg tatcgaacca tccacttcaa
      361 cgctgtcttt tgcgcctaat ccgttctcga caataaataa cggtttttga taacgatccc
      421 aaagcgtatt taacagaatc cgtaatccaa ccggatcaat ttgccacccc cactctgaac
      481 ttttcagatg cggatttggg atcatattca gtatgttgcc ctgcgcattt ttattaatgc
      541 tttcgtcgtg ggaaacacaa ccggtcatgt aataactaaa agagatgaaa tctacggtat
      601 gttttaaatc ttctgcgtca ctttcagtca tctcaatggt gatattgtgg tcgcggaaga
      661 aacgctgcat atagccggga tactgaccac gcgcctgaac atcaccaaag aacatccagc
      721 gccggttctc ttccatggcc tgcaacatat cctgtggctg gcaggtgagg gggtaaacca
      781 gcccaccgag aagcatattg ccaattttcg cttcggggat caggctatga caggctttaa
      841 ctgcccgcgc actggcaacc agttgatgat ggatcgcctg ataaacttcc gcctcgccac
      901 tctcttctgc cagccccacg cccgtgaatg gcgcgtgtaa tgacatgttg atttcattaa
      961 atgtcagcca taacacaact ttatgttggt agcgggtaaa gaccgtgcgt gcgtaatgct
     1021 cgaagtgatc gatgaccgcc cgattagccc aaccgccgta gtttttcacc agcccatatg
     1081 gcatttcgta atgggataac gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa
     1141 acagccgctc gtaaaacgct aaccccgctt cattcggttc ggcttcgtcg ccctgaggga
     1201 aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa gcccatctcg gcaaataacg
     1261 cgatatcttc cgggtaacgg tgataaaaat cgatggcgac atctttgata ttctctttcc
     1321 ccaggatgcg cggttccatt tttcccatta cgccatgagg ctgtaaatct gaggtcgtga
     1381 tccctttgcc atcttcctgc catgcgcctt ccacctgatt ggcagctgtt gcaccacccc
     1441 aaagaaatgt ttctggaaat gctttcataa ttaactcctt ttatcgttag cgaatgatgg
     1501 ataacagcgg ctcaccagcg tttatctgca ccgtgccgtg gggtaatacg tccgtaaaat
     1561 catcgctatt actgattaat accggcgtcg tcagatcaaa tccggcctcg cgaatagcag
     1621 ggatatcaaa agaaatcagc cgatcgcctg tattgacctt gtcacccacg ttgacgtgag
     1681 cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc gacatgaatc aggatctcca
     1741 cgccatcatc tgactcaatg ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac
     1801 ccgccaccgg agaacgcact tcaccaacag agggcagaat ggcaatacct ttacccaaca
     1861 ggccactggc aaacgtggta tcagcgacgt gaatgagcgg cacaatatct cccgtcatcg
     1921 gtgaacagat accgccctgc tcaggtgtaa taatctctgg tgttttctct tgctgggcaa
     1981 cttgcgctgg ctgacgttta gcggtgatga aatgaagcat caccgtaccg acaaatgcgc
     2041 aaccgatggc aatgacacca ccaataacgc tggcccatac ggtgaaatca atccccgttg
     2101 acgggatggt ttgcatgaag gtgaaaatac ctggcaaacc aaaggagtaa actttcgttt
     2161 gcgcgtagcc aatgatggtg gcccccaatg ccccacttat gcaggcgata acgaaggggt
     2221 acttacgcgg caggttgacg ccatatactg ccggttcggt gataccaaac agccccgtca
     2281 acgccgccga tcccgctacc acttttttct gcgcatcgcg ttcgcagagg aagacgccga
     2341 gcgccgcccc gacttgcgcc ataatggcgg gcattaacag cgggatcatg gtgtcgtagc
     2401 ccagcacggt gaagttattg ataattaaag gtatcaaacc ccaatgcaga ccaaacatca
     2461 caaagatttg ccagaagccg cccattaccg cgcctgcaaa tgcaggaacc gcctgataaa
     2521 gccagagata accggcagca atcagttcac ttatccaggt tgatagcggc cccaccagca
     2581 gaaatgtgat gggcgtgatg accatcagac atagcaaggg tgtgaagaaa tttttgattg
     2641 ccgaaggtaa ccacgcatta agtcggcgtt ccagaatgct gcacaaccag gcagaaaaaa
     2701 taatgggaat aaccgatgac gagtaattca acaatgtgac cggaataccc aggaaatcca
     2761 ggcccagcgc atccactttt tgcccgttct cgaaggcagt cagaattaat ggatgcacta
     2821 acgctccgcc aatcaccatg gcagtaaatg gattgccgct gaagcgtttc cctgcggtgt
     2881 agcccaggat tatcgggaag aaccaaaaca aggcatcact ggcgctaaat aaaattaaat
     2941 aagtaccact ttgttcggtc gtccactgaa aagtgagtgc cagagccaac atacctttca
     3001 ggatcccggt tgccgccatc aaaccgatca gaggcgtaaa aatacctgaa ataacataaa
     3061 caaagcggtt taacagatta cctttatcat cattttccgg tgcctgttgc gctttttcgc
     3121 caaggcctgc cacgctgtta accgccagga agacatcggc cacatggtta cctatgacaa
     3181 cctgaaactg gccaccgctt tccaccacca taataatacc gggggtcttt ttcagtacct
     3241 cagcttgcgc tttgctttca tcctttaact taaaacgtaa tcgcgttgca caatgcatca
     3301 gactcacaat gttatctgcg cccccgactc ctgcgactat tgttctggct aactccgtca
     3361 taacttgccc tctaccgctt tgcggcaaaa ctcaaaaaaa aacctgaaaa aaacggcctg
     3421 acgtgaatca agcaattttt ttcaggtttt gcccgcttag tgcggtaaca atcctttatt
     3481 cagtaataat atttcagtgt tctttgcgca cgcgctctat atttatggct aaaaacataa
     3541 tctctgcagg tgagatttta cgttgatact gcaaaccaat aaaaatggcg atccgctccg
     3601 cacattgcca tgcttgcggg taattttgct ttactgctta ttgtaatgat tcatcactat
     3661 cgttaatcga tgcatgttca ataatacgcc aggataaaaa tttcagatgc gtaaccagtc
     3721 gctgataact caagctttct tcctggtaat taaggctgaa ctgaaatttt attaattgca
     3781 gcatttctcg cattaattgc gtgacacctg caacatcctc catatttccg ctcatttggg
     3841 cactgaccag atgcatggca ataaagccca cttcatcttt cggtaactgc acgcccaacc
     3901 gtttatcaat aatggttaac gcttcttccc ctagctggaa ctctttcggg taaagtcgct
     3961 ggatatccca cagcagcggg ttgggcagta gcacgttttg ctgaaagcgt ttaatcgcaa
     4021 actggcaatg gtcagttagc gagatataaa tactgtcctg caactttccc agacgctcct
     4081 gcgccagaga gataatacga tcacaggttg ccatcacctc aagaggcata tgacttaaga
     4141 gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc atactctttt tctattccac
     4201 ttgagttaat tctttcgcct gggcgttttt gaaagccaat tccgcgcccc atgacgactt
     4261 tttcccgctg ttgatcatca ataaccacca caacattatt gttgagaatt ttggtgattt
     4321 gcatgttcat agcaaggacc ttttttataa acaaaaaaac ccgacttcac cagtattctc
     4381 tggttatgtc aggttttgcc tgcgaatgca gtaacaatcc agtcattaat taatggtttt
     4441 tataacgaac atccaggttc gaaaattaat ttagttgcgt gcagaaaaaa tagcaatgct
     4501 ctatttataa aagtatgacc atgctcgcag ttattaattt tgtgtaattt taggatttta
     4561 taaagttata tataacaaat cccaataatt aaattattgg gatttacttg gtgaattatt
     4621 tatcgctatc tttttctgcc agcagtttat cgagctcgtc accaccgacg tgacggaaat
     4681 cctgcccctt cacgtagtag aagataaact cacaaatatt ctggcagcgg tcgccaatac
     4741 gttcgataga acgcgcacag aataacgcgg taagtacgct cggaatggta cgcgaatctt
     4801 ccatcatgta ggtcatcagt tgacgaacaa taccttcgta ctcctgatcg acttttttat
     4861 cttcacgata aatacgtacc gcttcgtcaa tgtccatccg cgcgaacgcg tccagcacgt
     4921 cgtgcagcat ctggatggta tgacggccca gcgactccag acttaccagc aacggctgat
     4981 gctgctggga gaatttctcc agcgcagtac ggcagatttt atccgccacg tcaccaatac
     5041 gctccagctc ggcaatggtt ttactgatta ccatcaccag acgcaggtcg ctcgccgtcg
     5101 gctgacgttt ggcgataatg cgcacgcagg cttcatcgat cgccacttcc atcatgttga
     5161 cgttcttgtc gccttcgatg acgcgcttcg ccagatcgct gtcctggtta tgcatcgcgg
     5221 tgattgcatc agaaagctgc tgctccacca tgccgcccat ggtcatcacc tgcgtgcgga
     5281 tgctttccag ttcggcgttg aactggccgg aaatatgttt attaagattg agactgtcca
     5341 taacgcactc ctgaatcaac cgtaacgacc ggtgatgtag tcttctgttt gttttttcgc
     5401 tggcttggtg aacagatcgt ccgtgttgct gaactcaatc aattcgccca ggtacataaa
     5461 cgccgtgtgg tcggaacaac gcgcagcctg ctgcatgttg tgggtgacga tcaccacggt
     5521 gtaatcctgc ttcagttcgg tgatcagctc ttcaatacgc ccggtagaga tagggtcgag
     5581 tgccgaacac ggttcgtcga gcagcagcac ttccgggcga atggcgatac cacgcgcaat
     5641 gcacagacgc tgttgctgac caccagagag agagtaaccg ctctggtgca atttatcttt
     5701 ggtttcgttc cacaatgcgg ctttggtcaa tgcccactgc acgcgctcgt ccatgtcggc
     5761 acgggagagc ttctcaaaca gacgcacgcc aaaggcgatg ttgtcgtaaa tggacatcgg
     5821 gaacggcgtc ggtttctgga acaccatgcc cactttcgca cgcagcaggg cgatatcctg
     5881 agagttggtc aggatgttgt cgccatcgag cagaatttca ccttccgcac gctgctccgg
     5941 gtacagttca aacattttgt tgaaggtacg cagcagcgtt gatttaccgc agccggacgg
     6001 cccgataaac gccgtaacct ggtttttggc gatatccagg ttgatgtttt tcagggcatg
     6061 gaatttgccg tagtagaagt tcaaattacg aacctgaatt ttactcgggg cagtttcaac
     6121 catactcatt gcaatctttt cctcatcgtg cctgatgcac ttcgcgaatc aggcttaccg
     6181 taggccggat aaggcgttta cgccgcatcc agcaaagtgt taaccgtgtt tattcttcgc
     6241 aaaaacaacg cgcgccagaa tgttcagcag cagtacgcac agggtaatga tcaacacccc
     6301 ggcccaggcc agttgctgcc attccgcaaa cgggctcatc gcaaacttaa agatcgtcac
     6361 cggcaggttg gcgatcggct gcatcatgtc cgtgctccag aactggttgg agagcgcagt
     6421 aaacagcagc ggtgcggttt caccagcaat acgggcaatc gccagcagga taccggtcat
     6481 gatcccggac accgacgctt tcagcgtgat cgaggcgatc atcttccact tcggcgtacc
     6541 cagcgcataa gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt tctcggtggt
     6601 gcggataaca atcggcacct gcaacagcgc cagcgcaatc acgcccgccc agccggagaa
     6661 gtgctccatc tgcgccacca cgatggtgta aacaaaaaga ccaaccacaa tcgacggcgc
     6721 ggagagcaga atgtcgttaa tgaagcgaat cacttctgcc agccaggatt tacgaccata
     6781 ttcagccagg taaatccccg ccataatgcc cagcggcgta ccgaataccg tagcccacaa
     6841 aattaacagg ccgctacccg ccagggcgtt cgccagacca ccgccttccg tattgggtgg
     6901 tggcgtcatt tcggtgaaca gcgccagcga cataccgtcg ataccgcgag tgatggtgga
     6961 cattaaaatc cagatcagcc agaacagacc gaaggccatc gtcgccattg agagcgtcag
     7021 cgcaatacgg tttttgaggc ggcgacgcgc ctgcattttg cggcgggatt cagccagtgc
     7081 cgcagtggtt tgcatttcaa ccatagccat tagcgtgccc cctcattctt agccaggcgc
     7141 ataatcataa acttcgatgc ggcgaggacg atgaaggtaa tcacaaacag gatcaggccc
     7201 agttccatca gtgcagcaac gtgcagaccc gactctgctt cggcaaattc gttcgccagc
     7261 gcagaggtga tactgttgcc cggcatgtac agcgaggcgc tgtcgagctg gtaggtgtta
     7321 ccgatgataa aggtcaccgc catggtttca ccaagcgcgc ggcccagccc caacataatg
     7381 ccgccgataa caccattttt ggtgaacgga agaacgatac gccagataac ttcccaggtg
     7441 gtgcagccaa taccgtaggc cgactctttc atcatcaccg gggtttgttc gaacacatcg
     7501 cgcattaccg ccgcaatgta cggaataatc atgatggcga ggatcacgcc tgccgcgagg
     7561 ataccgatac caaaggccgg gccggagaac agcgcgccga caatcgggat attcgacatg
     7621 atattgccga ccggctcctg gaagtaaacg gcgaacagcg gcgcaaagat aaacaggccc
     7681 cacatgccgt aaacgatact tggaatggct gccagcagct caatggcgat acccagcggg
     7741 cgcttcagcc agccaggcgc aagctcggtc aggaacaggg cgataccgaa actcaccggg
     7801 acggcgatca gcagcgcgat aaacgaggtc accagcgtac cgtagatcgg caccagcgcc
     7861 ccgtagatat cgttcggcgc atcccactct ttggtccaca ggaaagccag accaaatttc
     7921 tgaatgctcg gccaggagga gatgatcaga gagacaataa tgccacccaa catcaatagc
     7981 acaatcagcg ccgccagttt taccagcacg ctgaaaatta tgtcgccctt tttacccggt
     8041 gggttaaaag caggcttggt tgcagccata agttactctt cagttaaacg cgtttacaaa
     8101 gtcattttgt aatgccggat gcggcgtaaa acaccgtacc cggcctgagt tttattagta
     8161 cagcggcttg ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa cctgttccac
     8221 tacgctatcc ggcaggctgg cgtaatccag gtcgttcgcc tgtttagccc cggttttgta
     8281 tgcccagtcg aagaacttca gcacttctgt gccttgttct ggtttcttct gatctttgtg
     8341 gatcagaatg aacgtggtag aggtaatagg ccacgcatct tcgcctttct ggttggtcag
     8401 atcctgagcg aatgttttgc tccagtctgc gccttttgct gcattagcga agttttcttc
     8461 ggtcggacta accggtttgc catcagcgga gatcaatttg gtgtacgcca ggttgttctg
     8521 cttcgcgtaa gcatattcaa cgtaaccaat tgcacccggc agacgctgaa cgaacgcggc
     8581 gataccgtcg ttacctttac cgcccagacc gatcggccat tttacggtag agccagtacc
     8641 aacgttgttt ttccactctt cgttcacttt cgccaggtag ctggtgaaga cgaaggaagt
     8701 cccggagcca tctgcgcggc gtactacagc aatgttctgt gaaggcagtt tcagacccgg
     8761 attcagtttg gcgatggctt catcatccca cttcttgatt ttgcccaggt agatgtcgcc
     8821 gagggtttta ccatccagca ccagttcgcc agacttcaga cctggaatat taaccgccag
     8881 caccacgccg ccaatcacgg tcgggaactg gaacaggcct tcctgagcca gtttttcatc
     8941 agacagcggc gcgtcagagg caccgaaatc aacggtgtta gcgataatct gttttacgcc
     9001 accggaagaa ccgataccct ggtagttaac tttattaccg gtttctttct ggtaagtgtc
     9061 agcccatttg gcatacaccg gcgcagggaa ggttgcacct gcacctgtca ggcttgcttc
     9121 tgcaaacaca gagaaagcac tcatcgataa ggtcgcggcg acaacagttg cgacggtggt
     9181 acgcataact ttcataatgt ctcctgggag gattcataaa gcattgtttg ttggctacga
     9241 gaagcaaaat aggacaaaca ggtgacagtt atatgtaagg tttatgacag ttttatgaca
     9301 gagagagaaa gtctttagtc tgatttaaat aagcgttgat attcagttaa ttacaaatat
     9361 taataacgaa gagatgacag aaaaattttc attctgtgtc agagaaaaag tagccgaaga
     9421 tgacggtttg tcacatggag ttggcaggat atttgattaa aaacataaca ggaagaaaaa
     9481 tgccctgctt acgcagggca tccatttatt actcaaccgt aaccgatttc gccaggttac
     9541 gcggctggtc aacgtcggtg cctttgatca gcgcaacgtg gtaagccagt agttgcagcg
     9601 gaacggtgta gaagatcggt gcaatcacct cttccacatg cggcatctca atgatgtgca
     9661 tgttgtcact gctcacaaaa cccgcatcct gatcggcgaa gacatacaac tgaccgccac
     9721 gcgcgcgaac ttcttcaatg ttggatttca gtttttccag caattcgttg ttcggtgcaa
     9781 cgacgataac cggcatatcg gcatcaatca gcgccagcgg accgtgtttc agttcgcctg
     9841 cagcgtaggc ttcagcgtga atgtaagaga tctctttcag cttcaatgca ccttccagcg
     9901 cgattgggta ctgatcgcca cggcccagga acagcgcgtg atgtttgtca gagaaatctt
     9961 ctgccagcgc ttcaatgcgt ttgtcctgag acagcatctg ctcaatacgg ctcggcagcg
    10021 cctgcagacc atgcacgatg tcatgttcaa tggaggcatc cagacctttc aggcgagaca
    10081 gtttcgccac cagcatcaac agcacagtta actgggtggt gaatgctttg gtggaggcca
    10141 cgccaatttc cgtaccggcg ttggtcatca gcgccagatc ggattcgcgc accagagaag
    10201 aacccggaac gttacagatt gccagcgaac caaggtaacc cagctctttc gaaagacgca
    10261 ggcccgccag ggtatccgcg gtttcgccag actgtgacaa ggtgatcatc aggctgttac
    10321 gacgcacggc agatttgcga tagcggaatt cagaggcgat ttcgacgtcg cacggaatac
    10381 ctgccagcga ttcaaaccag tagcgggaaa ccataccgga gttataagaa gtaccacagg
    10441 cgaggatctg aatatgctca accttcgaca gcagttcatc ggcgttcggt cccagctcgc
    10501 ttaaatcaac ctgaccgtgg ctgatgcgcc cggtaagggt gtttttgatc gcgttcggct
    10561 gttcgtagat ctctttctgc atgtagtggc ggtaaatacc tttatcgccc gcgtcatatt
    10621 gcagattgga ttcgatatcc tgacgtttta cttccgcgcc agttttatcg aagatgttta
    10681 ccgaacggcg agtgatttcc gcaatatcgc cctcttcaag gaagataaaa cgacgagtca
    10741 ctggcaacag cgccagctgg tcagaagcga taaagttttc gcccataccc aggccaatca
    10801 ccagcggact accagaacgt gccgccagca gggtatccgg atgacgggag tccatgatca
    10861 ctgtaccgta cgcaccacgc agttgcggga tagcacgcag aactgcctca cgcagagtac
    10921 cgccttgttt cagctcccag ttcaccaaat gtgcaatcac ttcggtgtcg gtttcagaaa
    10981 cgaaggtata gccacgcgct tttaactctt cacgcagcgg ttcatggttt tcgatgatgc
    11041 cgttatgcac caccacaatg tgttcagaaa catgcggatg cgcgttagct tctgaaggtt
    11101 caccgtgggt cgcccagcga gtgtgagcaa taccggtgcc gccatgcaga ggatgttctt
    11161 ccgctgcctg agccagcatc tgtactttac cgaggcgacg caggcgggtc atatgacctt
    11221 cagcatccac cacggccagg ccggcagagt catatccgcg atattccaga cgacgtaaac
    11281 cttcaagaag gatttctgct acatcacgtt gcgcgatcgc gccaacaatt ccacacatag
    11341 tttttgattc cgatttatat cgttgtcggt caacctgtat gcccgttttt tcgggcgccc
    11401 cgagccttgt agagagtggg gttattttta tagttactgc ttgtgggcgg gagattatgt
    11461 tatctcctca tcccatatga ccggattacc cgaccagaat tactttttct ttatcggacg
    11521 acgccagcct tctttttgag tctgcggcac acggctgata gccagggcat tttcgccgac
    11581 attacgcgtc acagttgtac ccgcagcaat ggtcgcgcct ttgcctactg ttaccggggc
    11641 caccagttga gtgtcggaac cgacaaacac atcgtcgccg ataatggtct taaatttatt
    11701 cgcaccatcg tagttgcagg taatggttcc cgcaccgatg ttaacgttat cgccaatttc
    11761 cgcatcgccc aggtaagtca gatgaccagc tttcgagcct ttacccagac gcgctttttt
    11821 catctcaacg aagttaccga cgtgtgcacc ttccagcaac tcagcaccag gacgcagacg
    11881 ggcaaacggg ccaatagtac aggccgctgc cagattcgca tcttccacga cggtatatgg
    11941 actgatttcg caatcatcgc caatcacgct gtttttaatc acgcaaccgg tgccgatttt
    12001 cacgcgatga ccgagagtca cgttgccctc gatgataacg ttagtatcaa tttcaacatc
    12061 gcgcccgtga gtaagcgtac cgcgcagatc aaaacgcgcc ggatcgcgca gcataacgcc
    12121 tgctaacagc agtttttcag cctgttcgga ctggtaaacg cgctccagtc gggagagttg
    12181 caggcggtta ttcacgcctt ctacttcgct taaacgttgc ggatgaacgg cgacgatttc
    12241 acgtccttcc tgatacgcca gcgcaataat gtcggtgatg tagtattcgc cctgagcatt
    12301 attgttggtc agcttcgcca gccagcgttt catatctgcg ccgttggcaa tcagaatgcc
    12361 ggtgttgatc tcctgaatct gacgctgctc gtcggtggca tctttgtgtt caacaatgcc
    12421 ggtaactttg ccgttttcac gggtgatacg tccataaccg gtcggatcat ccagttttac
    12481 cgtcagcaga ccaatgccac cctgcggttt agcatcacgc agacgctgga gtgtttcgac
    12541 agagatcagc ggcacgtcgc cgtagagcat taaaatgtct tcatcatcgg caaagaaagg
    12601 tgcggcctgc tgcatcgcat gacccgtacc cagctgttct gcctgaagca cccagttcag
    12661 gttgtcgtct ttcagcgcct gcttaagcag atcgccgccg tgaccataca caaggtgaac
    12721 gtgcgctgcg cctaattcat tcgcagcatc aatgacatgc tgaaccatcg ctttcccggc
    12781 aagggtatgc agcactttcg gaagatcgga atacatgcgc gtgcctttgc ctgcggcaag
    12841 gatcactacg ctcatagcat tattcaacat acgcgtcctg actgtaattt gagaacgaat
    12901 ttaaaccgct tcaccttgaa aaaactacat ttttttcatc gtgaaatgga cagaggataa
    12961 attgttcaat aacggattat ccccgctgac gaaagcgcca ttttcgacca tcgtcacgcc
    13021 ttttgtctgc agaaaattaa ggcagtacct tcatttaagc gtcaggtgga tgtttttgct
    13081 cttatttcga tcaatgaata aacgggaaaa caggggtgtt ttatatcatc aggatctatg
    13141 tgaacgcttt tcaggacggg tcaatacgcg taaaaaaaag ccagcctgtt gccagactgg
    13201 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
    13261 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
    13321 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
    13381 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
    13441 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
    13501 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
    13561 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
    13621 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
    13681 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
    13741 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
    13801 ccggagaacc ggtgaatacc tctgccacga agaacggctg ggacaggaag cgctggatct
    13861 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
    13921 cgataatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
    13981 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
    14041 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
    14101 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaggtc atccgcaggt acgtataccg
    14161 cctgtacgga ggtgatagaa ccggttttgg tggaggtgat acgttcctgc agaacgccca
    14221 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
    14281 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
    14341 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
    14401 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
    14461 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
    14521 cagagtaacc ggagtgctcg atcgcgatgt tacgaataag ctccatcatg tttacggttt
    14581 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
    14641 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
    14701 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
    14761 ccggttcacc cagtacgttc atgatacgac ccagagtcgc tttacctacc gggacttcga
    14821 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
    14881 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
    14941 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
    15001 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
    15061 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
    15121 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
    15181 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
    15241 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
    15301 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
    15361 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
    15421 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
    15481 cggaccgatc agttcggaca aggaagggtt atcccccatg ccggtgacct gggcaacaac
    15541 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
    15601 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
    15661 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
    15721 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
    15781 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
    15841 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
    15901 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
    15961 cctgcccaaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
    16021 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
    16081 atcaacggag cgtgatcacg gtcgacgtaa gccaacagag cggcttcgaa gctgccgatt
    16141 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgt
    16201 tgcgcaacag acatcggcgc atactgtttc tgcttcagca gttcggtcac tttctgaccg
    16261 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
    16321 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
    16381 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
    16441 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
    16501 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag cgcagtcaga
    16561 gaaccggttt tccctttcac ttcacctttg gtgaaagctt caacgtattc ggcgttaaca
    16621 cgcgcagcac gctccagcag acgagagtgg aggtagaata cgtcgcccgg gaatgcttca
    16681 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
    16741 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc acccattgcg
    16801 caaccagcgt acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
    16861 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
    16921 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
    16981 atggcatcga tagccagtgc ggttttaccg gtctgacggt caccgatgat caattcacgc
    17041 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
    17101 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
    17161 tgatccagcg gacctttacc atcgattggt gcacccagag tgttaaccac acggcccagc
    17221 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
    17281 gcaaggtcag cgtacggccc cataacaacc gcacctacag agtcgcgctc gaggttcagt
    17341 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
    17401 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
    17461 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
    17521 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
    17581 cgtacgctgc catcaatgac catatcaccc gctcggatga taacacctgc cattacagac
    17641 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
    17701 attttcgcga gctgttgttc actcaacgcg gcagcggaaa tgacgtctac ctcagcggta
    17761 gcctcactca cagcacgcag gtgaataaac tgctccagaa catccgggag cgcgttaaga
    17821 cgaccatttt cggccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
    17881 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
    17941 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
    18001 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
    18061 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
    18121 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
    18181 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttccg
    18241 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcatcca
    18301 gaatctgcga gcggcgtttg ttagcctgct cgatgattac ctgggcttcc gctttcgctt
    18361 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
    18421 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
    18481 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
    18541 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
    18601 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
    18661 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
    18721 ggaatcagat caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata
    18781 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
    18841 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
    18901 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
    18961 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
    19021 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
    19081 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
    19141 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
    19201 gaagccgccg atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag
    19261 tgccatagac agcgttacgt tcacgtcagc agacggaacc acacgcagtg caggcagacc
    19321 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
    19381 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
    19441 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    19501 cttacctggc acgccgctgg ttgccttttt ggctacacta cggaataaaa ccaggaacaa
    19561 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    19621 ggggttatgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    19681 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    19741 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    19801 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    19861 agaactttga aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta
    19921 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    19981 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    20041 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    20101 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    20161 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    20221 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    20281 tacaattgcg acttttctgc aaaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    20341 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    20401 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    20461 gttttaataa atgacattta cataacaaaa accagccatt gacattttta ataatgtttt
    20521 aacagtcaat gatgattctt agcgccgatt tttagcagac tgatattttc acttatgact
    20581 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc
    20641 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    20701 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    20761 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    20821 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    20881 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    20941 aattacgcca tcaaatggcg gctctgacgg aaactcttct accctgctct gtactggctc
    21001 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    21061 gctatccaac aacgtgaaat gagcttcagg acgcacgata gagagtggaa tgccaggcag
    21121 tcccggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    21181 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    21241 cgctttattc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    21301 ggtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    21361 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    21421 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg
    21481 cgtgacgcca gaaatacgcg aagcctggcc aatagaggcc ggtttgtgat cgttaagttt
    21541 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagcgtcg ctggtagcag
    21601 ggtattctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    21661 gtatttaacc tgaatctcaa cctgttccgc agcctgttca tctgtcaacg caggggcaaa
    21721 cggtgtcagc gtggttaatt tttcataagt catttcagga cgacgtagca gatcttcacc
    21781 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    21841 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    21901 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    21961 caaacgcaga tcagcattat cttcgcgcag catcagacga tattctgcgc gcgaggtaaa
    22021 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    22081 cgcctgagaa cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    22141 accagccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    22201 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc
    22261 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    22321 catggagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    22381 ataaatttcg ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc
    22441 ggcaaagcgc atgactttgt cttcaatcga cgggcagtag cgtgggccga caccttcgat
    22501 cacccctgcg tacattgggc tacgatcgag attactgcgg atcacatcat gggttttctc
    22561 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    22621 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agtacgctaa agtcgatggt
    22681 tcgagcatca atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc
    22741 acgcaaacgg cgagaaagcg gaatggacgg cggatcacca gcacggccac cgctgtaatt
    22801 atccagaccg atatgaattt taccgtcgag gaacgtccca acggtgagca cgacagcttt
    22861 ggcacggaac ttcagtccca tttgggtaac ggcaccgacc acgcgatcgt tttcgacaat
    22921 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    22981 taccgcctga cggtagagca cacgatctgc ctgagctcgg gtagcgcgaa ccgccggtcc
    23041 cttgcttgcg tttagtatcc taaactggat acccgcctga tcgatcgctt tcgccatcag
    23101 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    23161 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    23221 catacgcgcc gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    23281 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    23341 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    23401 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    23461 tcgcactgcc ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc
    23521 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    23581 tacacaactc aaaaactgaa caatcgttgt tctttggata actaccgttt gatccaagct
    23641 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    23701 ccacgatccc agccattctt ctgccggatc ctccggaatg ttgtgatcaa gaatgttgat
    23761 cttcagtgtt tcgcctgtct gttttgctcc ggaatttttg agttctgcct cgattttatc
    23821 gatagcccca caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac
    23881 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    23941 aatatctccg gcaccgtggg ttgagctgat aaccagccag atccccgagg cagataaatc
    24001 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    24061 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    24121 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    24181 ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg gcttaatggt acgcatgatc
    24241 gggttctgca ggacgatcag tgtctcggtg gactgaattt catcaattgt ttggatcttg
    24301 ttgataagta catgctggag agcatcgatc gaacggcaca tcacttttat aaagatgctg
    24361 tagtggccgg ttgtgtaata ggcttcagtg acttcatcga ggctttccag ctttgccagc
    24421 gcggaaggat agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    24481 ccaagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    24541 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    24601 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    24661 agataatttt ccataggatt ttcttatgcg gattgatgat tcattctatt ttagccttct
    24721 tttttaatga atcaaaagca cgtgaggctt tttattgaat gattattgca tgtgtgtcgg
    24781 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    24841 attgccaaac aacgtcaaat tagcttcgtg aaatctcact tttctcgtca actggaagaa
    24901 cgtctggggc tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag
    24961 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    25021 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    25081 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    25141 ctttccccgt tgcactcggt ctatgtagac cagtgggact gggaacgcgt aatgggcgac
    25201 ggcgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    25261 gcaaccgaag ctgcggttag caaagagttt ggcctggcac cgttcctgcc ggatcagatc
    25321 cacttcgtac acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt
    25381 gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagttg
    25441 agtgatggtc atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    25501 gagctgggcc atgcgggtct gaacggcgat attctggtgt ggaacccggt actggaagat
    25561 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    25621 gcgctgaccg gtgacgaaga tcgcctggag ctggagtggc atcaggcgct gctgcgcggt
    25681 gaaatgccgc agaccatcgg cggcggtatc gggcagtctc gtttgaccat gctgctgctg
    25741 caactgccac atatcggcca ggttcagtgt ggtgtatggc cggcagctgt tcgcgagagc
    25801 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    25861 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccatgtgcag
    25921 acatcgccac ggcatgaaag cgatgctgat gtacccgctg tagctctttc actttactcg
    25981 tcacgtcgtc aggcaaccgc tgggcgataa aatcagaaat caccaccgca tcggcatcaa
    26041 accattcccg gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    26101 cgccacgaaa ctgctgactt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    26161 gctcataacg gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga
    26221 gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    26281 ttgagccgga agtatccaca cagacaataa acggtccgcg cggctgttcg tcgtaatctt
    26341 tatgcaccac cgggcgttcg atcacttttt cacgccacga ctcaccatgc aggcgatagg
    26401 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttct gttatcccta
    26461 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    26521 cctgctcagg aaccgtcgcc ggttcgcgca ccagggtgcg gaaggtttcc atctgcgcat
    26581 cgttgcgcgg tattgatttg gcttcccggg aacgccccag ctgttctgcc aggcgtttca
    26641 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    26701 taagctgacc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    26761 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcactcaac agttgttcgc
    26821 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    26881 aacgctggag aaaaagcgtg tgtaacgctg aagtgatcgt gctgttagca tcaaccaact
    26941 gtcgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    27001 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    27061 cggtgagttc cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    27121 ggggaacatc atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagactgcca
    27181 gctgcggtga ggccagcagc gcgatgatca tctcttcgat caatccctct tcgctgacgg
    27241 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcgcc
    27301 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    27361 ttgctgttcg ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg
    27421 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    27481 ttgtaaactt acatcgcgta caacaagatg ttgggcgcta tccacttcca gattcagttt
    27541 ctgggcaaag ccgataccgt tcagtttgcc gcgaatctca ccacctttac tcagccactg
    27601 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca attttaacgg
    27661 tttttgcagc agcagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg
    27721 acgacggctg aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg
    27781 ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg cgggtcaaca tcccttgctg
    27841 ttgccaggca tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    27901 cgcgtcatac cacaggcaat ctttcagcaa aatgagatca accggggcaa cagcactgcg
    27961 accgctaaaa aaggcgctgg cctgcaataa gcggatcgct tttttccagc gacgatccga
    28021 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    28081 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    28141 tgtgatctgc aaggaggcag gaaccggatt gtcattttca tcctgttgac tggtcagcat
    28201 ggagcggaaa ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc
    28261 atataacgct tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag
    28321 acgcatcggg attttttcta cgagtgcgcc gttgcggaac tggcgctcgt taatggcggt
    28381 gagcaaggta ttaagaattg ccggacccgc tttccagatc tcatccagaa agacgatttc
    28441 agcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    28501 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa
    28561 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    28621 tggtgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    28681 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    28741 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcatcaaaa gttctgcgta
    28801 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaagga ggggtatttt
    28861 tctttgagcc aggttaatgt ggccgcattt aggagtacga ttttgccgtt aatcgtgcat
    28921 actgtgcgct tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac
    28981 tagtctatga gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga
    29041 gttgtctacg gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc
    29101 cagtttggtt ttggcgttga acgcgatgcc gtgtttggct ttttatcgct gatcttctgg
    29161 ctgctaatct ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc agataacgcc
    29221 ggtgaagggg ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc
    29281 acatcaatgc tggtgattat ggggctaatc ggcggcagct ttttctatgg tgaagtcgtc
    29341 attactcccg ctatttcggt gatgtccgcc attgaaggtc tggaaatcgt cgccccgcag
    29401 ctggatacct ggatagttcc cctctcaatt atcgttctca cattattatt catgattcaa
    29461 aaacatggca cagctatggt cggtaagctg tttgcgccga tcatgctgac ctggtttttg
    29521 attctggcag ggctggggtt acgtagcatt attgctaacc cggaagtgct gcatgcactg
    29581 aatccgatgt gggcggtgca tttcttcctt gaatacaaaa cggtttcttt tattgcatta
    29641 ggggcagtgg tgctgtcgat tacgggggtc gaggcgctgt atgctgatat ggggcacttt
    29701 ggtaagttcc ctattcgtct ggcgtggttc accgtcgtat tgccttcctt aacccttaat
    29761 tacttcggcc agggagcgct gttgttaaag aatccggaag cgattaagaa cccgttcttc
    29821 ctgttggcac cggactgggc gctgatcccg ctgctgatca tcgccgcact ggcgacggta
    29881 attgcctcgc aggcggttat ctctggcgtc ttctcattga cgcgtcaggc ggtacgtctg
    29941 ggatatttgt cgccgatgcg cattattcac acctccgaaa tggagtcagg gcaaatctat
    30001 attccctttg tgaactggat gctctatgtc gcggtcgtga ttgtgattgt cagctttgag
    30061 cactccagca acctggcggc ggcgtacggg attgcggtga ccggaaccat ggtgctgacg
    30121 tctattctct cgactaccgt ggcacgtcag aactggcact ggaataagta ttttgttgcg
    30181 ctgatcctga ttgctttcct ttgtgtcgat attcccttgt tcaccgctaa cctcgataaa
    30241 ctgctctccg gtggctggtt gccattgagc ctcggtacgg tgatgtttat cgtgatgacc
    30301 acctggaaga gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg taactctctg
    30361 gaagcgatga ttgcttcgct ggagaaatca ccgcccgttc gcgtgcctgg gaccgcggtg
    30421 tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga tgcataacct taaacataac
    30481 aaggtattgc atgagcgggt gattctgtta actctgcgca ccgaagacgc gccatatgtc
    30541 cataacgtcc gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg cgtggtggca
    30601 agttatggtt ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg cggtctggaa
    30661 ggattaagct gccggatgat ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc
    30721 ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat
    30781 gcgctgcggg caccggatca atttgaaatc ccgccaaaca gagttatcga actgggtact
    30841 caggtcgaaa tctaacgcca gacgcctcct ttctccataa gggggcgttt ttgttttcat
    30901 ggttaatcac catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt
    30961 ttcccaactc agtcaggatt aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa
    31021 aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg gtgatctccc gactgggaca
    31081 taccgatacg ctggtggtgt gtgatgctgg tttacccatc cccaaaagta caacgcgtat
    31141 cgatatggca ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg tcgtcacaaa
    31201 tgaaatgcag gtcgaggctg tcattatcgc ggaagagatc aaacaacata atccgcaact
    31261 ccacgaaacg ttgctcactc accttgagca gctgcaacaa caccagggaa acaccattga
    31321 aattcgttac accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat
    31381 tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg
    31441 aggccgtcat ggaagcatta cttcagctta aaggcatcga taaagccttt ccgggcgtaa
    31501 aagccctctc gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg
    31561 aaaacggcgc gggtaaatcc accatgatga aagtgcttac tggtatctat actcgcgatg
    31621 ccggtacgct tttatggttg gggaaagaaa cgacatttac cgggccgaaa tcttcccagg
    31681 aagccgggat tgggattatc caccaggaac tgaacctgat cccgcagtta accattgccg
    31741 aaaacatttt cctcggtcgt gagtttgtta atcgctttgg caaaattgac tggaaaacca
    31801 tgtatgccga agcggataaa ttgctggcta aacttaacct gcgctttaaa agcgacaaac
    31861 tggtgggtga tctttccatc ggtgaccagc aaatggttga aatcgccaaa gtgctgagct
    31921 ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc gctgaccgat accgaaaccg
    31981 aatccttgtt ccgcgtcatc cgcgagctga aattgcaagg ccgcggtatt gtctatatct
    32041 cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt taccgttttt cgtgacggac
    32101 aatttattgc tgagcgtgaa gtggcatcac tgaccgaaga ttctctgatt gagatgatgg
    32161 tgggccgcaa gctggaagat caatatccgc acctggacaa agcgccggga gatatccgcc
    32221 tgaaagtcga taatctctgc ggacctggcg ttaacgatgt ctcttttact ttacgcaaag
    32281 gcgaaattct tggcgtctct ggtttgatgg gcgctgggcg taccgaactg atgaaagtgc
    32341 tctacggcgc actgccgcgc acaagcggtt acgtcaccct ggatgggcat gaagtcgtta
    32401 cccgttcgcc gcaggatggc ctggcaaacg gcattgttta tatctccgaa gaccgtaaac
    32461 gtgacggttt agtgctgggc atgtcagtaa aagagaacat gtcgctgacc gcgctgcgct
    32521 acttcagccg cgctggcggc agtttgaagc atgccgatga acagcaggct gtgagtgatt
    32581 tcattcgtct gtttaatgtg aaaacgccat cgatggaaca ggcaattggt ctgctttccg
    32641 gtggcaatca gcaaaaagta gcgattgccc gtggtctgat gacacgccct aaagtgttga
    32701 tcctcgatga gcctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga
    32761 ttaaccagtt caaagccgat ggcttgagca tcattctggt gtcatcggag atgccagaag
    32821 tattaggcat gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta
    32881 ctcgtgagca ggccacccaa gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg
    32941 tgaatcagga gtaaaaaaat gacaacccag actgtctctg gtcgccgtta tttcacgaaa
    33001 gcgtggctga tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg
    33061 acgttaagcc cgaacttttt caccatcaat aacttattca atattcttca gcaaacctcg
    33121 gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg tatcgacttg
    33181 tcggtaggtt ctctgttggc gctgaccggc gcagttgctg catctatcgt cggcattgaa
    33241 gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag gtgccgcaat tggtgcggta
    33301 actggggtga ttgtagcgaa aggtcgcgtc caggcgttta tcgctacgct ggttatgatg
    33361 cttttactgc gcggcgtgac catggtttat accaatggta gcccagtgaa taccggcttt
    33421 actgagaacg ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg
    33481 ccagtctgga tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt
    33541 ctggggcgtt acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc
    33601 aacgtcaata aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc
    33661 gggatcattg aagtggcgcg tctctcctcc gcacaaccca cggcggggac tggctatgag
    33721 ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc
    33781 attgttggga cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg
    33841 ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg
    33901 ctggtagaca acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa
    33961 aaactggcta ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg
    34021 gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg
    34081 aaagatggcg cgcagaaaga ggcggataaa cttggctata acctggtggt gctggactcc
    34141 cagaacaacc cggcgaaaga gctggcgaac gtgcaggact taaccgtccg cggcaccaaa
    34201 attctgctga ttaacccgac cgactccgac gcagtaggta atgctgtgaa gatggctaac
    34261 caggcgaaca tcccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc
    34321 cacatcgcat ctgataacgt actgggcggc aaaatcgccg gtgattacat cgcgaagaaa
    34381 gcgggtgaag gtgccaaagt tatcgagctg caaggcattg ccggtacatc cgcagcccgt
    34441 gaacgtggcg aaggtttcca gcaggccgtt gctgctcaca agtttaatgt tcttgccagc
    34501 cagcctgcag attttgatcg caccaaaggc ttgaacgtaa tgcagaacct gttgaccgct
    34561 catccggatg ttcaggctgt attcgcgcag aatgatgaaa tggcgcttgg cgcgctgcgc
    34621 gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat
    34681 ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag
    34741 attggcgcga aaggcgtcga aaccgcagat aaagtgctga aaggagagaa agttcaggct
    34801 aagtatccgg ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc
    34861 tgatggtgac ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca
    34921 cgcgccaccc taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca
    34981 gcctcgttgt tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta
    35041 ctccaggcga aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga
    35101 atcaggcggt ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgcactggcg
    35161 atgacagcat tggtgagagc gttcgccagc agctcgccac tgataatatc gatatttctc
    35221 cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag
    35281 gtgagaatgt catcggtatt catgccggcg ctaatgctgc cctttccccg gcgctggtgg
    35341 aagcgcaacg tgagcgtatt gccaacgcgt cggcattatt aatgcagctg gaatcaccac
    35401 tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta
    35461 acccggctcc ggctcgcgaa cttcctgacg aacttctggc gctggtggac attattacgc
    35521 caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag
    35581 cgaaggcggc gcaggtactg catgaaaaag gtatccgtac tgtactgatt actttaggaa
    35641 gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc
    35701 aggctgtcga taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc
    35761 tggaagaaaa aacattgcca gaggcgattc gttttgccca tgcagccgct gcgattgccg
    35821 taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag
    35881 acaggcagag gtgacgcttg gctacaatga aagatgttgc ccgcctggcg ggcgtttcta
    35941 cctcaacagt ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attacagcca
    36001 aagttgaagc ggcgattaaa gaactcaatt acgcgccatc agctctggcg cgtagcctca
    36061 aactcaatca aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt
    36121 cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt cttgtccttt
    36181 gcaataccga aggcgatgaa cagcggatga atcgtaatct ggaaacgctg atgcaaaaac
    36241 gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca gccttcgcgt gaaatcatgc
    36301 aacgttatcc gacagtgcct accgtgatga tggactgggc tccgttcgat ggcgacagcg
    36361 atcttattca ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata
    36421 aaggtcatac ccgtattgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc
    36481 ggttggaagg ttatcgggct gcgatgaaac gtgcgggtct caacattcct gatggctatg
    36541 aagtcactgg tgattttgaa tttaacggcg ggtttgacgc tatgcgccaa ctgttatcac
    36601 atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc
    36661 aggcgttata tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg
    36721 atatcgaact ggcaagcttt atgacgccac cattaacaac tatacaccaa ccgaaagatg
    36781 aactggggga gctggcgatt gatgtactca tccatcggat aacccagccg accctacagc
    36841 aacaacgatt acaacttact ccgattctga tggaacgcgg ttcggcttag atttacgctg
    36901 tcttttgatc aaattattac catcggttgt tttcagtagc atgaacattg ctgctgaagc
    36961 aacagtaata atgcccatcg tgataaacgt atagtggaat tgttcgactg tcgttgtgcc
    37021 ttccatccct tcgtaaacgc gaaggacggc cgcacttaca gcaacgccta aactgataga
    37081 cagttgctgc gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc
    37141 ggcaagtgtg atggtattca tcgcggtaaa ttgcgtcgac atagccatcc ctaatataaa
    37201 caacggcaag atcagcatcc atatcgccat tgctggtgat tgcaaagaga actgagcgat
    37261 cattagccca ataatcaccg tgatccccac taacgtatgg cgatagccca gacgacgtaa
    37321 gacttgggta accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca
    37381 gccagcaata aacgcctgat acccaaatcc tacctgtaac atcaatggca taaggaacgg
    37441 tacacagccg gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg
    37501 ggttttaaat aaatctaatg aaattaatgg gtttggcgtg cgtcgcgcat ggagaatata
    37561 gagaagcagt aaccagatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat
    37621 cttttccccg aatagctcta ttccgcttga gaagagaaca aggctgaggc caaacagcaa
    37681 aaagccagtg gtatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc
    37741 gtaaagaagg cccgcaatac ctatggggat attgattaaa aatatccagt gccaggttgc
    37801 ccaggtgacc agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat
    37861 ggcgacaaaa ttcaatacag gaagaagttc attacgagga taagcgcgca gtaaggccag
    37921 ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga ataacccgga agacaaccag
    37981 ctgtggtagc gagttggaaa gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc
    38041 aagggtaaaa atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag
    38101 catcgccacc gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt
    38161 aaggctatga gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat
    38221 gaagaaggcc atcgccgcga tccacggcaa acccgccata ctgcgcttct ttttatcgct
    38281 cattcaaagt cctgttatcg ggttatcact tatctggtga gcgtagcagc gcctgacaag
    38341 ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc gacaatcgcc tggtgcagat
    38401 ccagctttat cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga
    38461 atagcgaggc gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc
    38521 aggccatatc gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt
    38581 cggccattaa cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gctgccagta
    38641 ggcaagcttg cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa
    38701 agttctcttc tgtcatccac caggtaagca attcctgatc aagaaaattc cagttcgatt
    38761 gtggcatgac tcgggtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg
    38821 ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct
    38881 caccaggcaa aatggtgccg ggttcatatt caccttttaa gatccgctgc gccagctttt
    38941 cagccagaac atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta
    39001 ctaatcttcc tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt
    39061 tgcaaaaacg gcaaattgct tgttttatgg cacattaacg gggcttttgc t
//
LOCUS       DAOFSV010000034        38391 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.034, whole genome shotgun sequence.
ACCESSION   DAOFSV010000034 DAOFSV010000000
VERSION     DAOFSV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.034"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(98..913)
                     /gene="djlA"
                     /locus_tag="QBH27_001826"
     CDS             complement(98..913)
                     /gene="djlA"
                     /locus_tag="QBH27_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDQ7014147.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGSGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1168..3522
                     /gene="lptD"
                     /locus_tag="QBH27_001827"
     CDS             1168..3522
                     /gene="lptD"
                     /locus_tag="QBH27_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDQ7014148.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            3575..4861
                     /gene="surA"
                     /locus_tag="QBH27_001828"
     CDS             3575..4861
                     /gene="surA"
                     /locus_tag="QBH27_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDQ7014149.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            4861..5850
                     /gene="pdxA"
                     /locus_tag="QBH27_001829"
     CDS             4861..5850
                     /gene="pdxA"
                     /locus_tag="QBH27_001829"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDQ7014150.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGLLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            5847..6668
                     /gene="rsmA"
                     /locus_tag="QBH27_001830"
     CDS             5847..6668
                     /gene="rsmA"
                     /locus_tag="QBH27_001830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDQ7014151.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVGVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            6671..7048
                     /gene="apaG"
                     /locus_tag="QBH27_001831"
     CDS             6671..7048
                     /gene="apaG"
                     /locus_tag="QBH27_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDQ7014152.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7055..7903
                     /gene="apaH"
                     /locus_tag="QBH27_001832"
     CDS             7055..7903
                     /gene="apaH"
                     /locus_tag="QBH27_001832"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDQ7014153.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(7930..8214)
                     /locus_tag="QBH27_001833"
     CDS             complement(7930..8214)
                     /locus_tag="QBH27_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDQ7014154.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLIDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIK"
     gene            complement(8217..8450)
                     /locus_tag="QBH27_001834"
     CDS             complement(8217..8450)
                     /locus_tag="QBH27_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDQ7014155.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(8536..9015)
                     /gene="folA"
                     /locus_tag="QBH27_001835"
     CDS             complement(8536..9015)
                     /gene="folA"
                     /locus_tag="QBH27_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDQ7014156.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9207..11069)
                     /gene="kefC"
                     /locus_tag="QBH27_001836"
     CDS             complement(9207..11069)
                     /gene="kefC"
                     /locus_tag="QBH27_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDQ7014157.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRRAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11062..11592)
                     /gene="kefF"
                     /locus_tag="QBH27_001837"
     CDS             complement(11062..11592)
                     /gene="kefF"
                     /locus_tag="QBH27_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDQ7014158.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11700..13031)
                     /locus_tag="QBH27_001838"
     CDS             complement(11700..13031)
                     /locus_tag="QBH27_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7014159.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWTLPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13090..13377)
                     /gene="fixX"
                     /locus_tag="QBH27_001839"
     CDS             complement(13090..13377)
                     /gene="fixX"
                     /locus_tag="QBH27_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDQ7014160.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKRDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(13374..14660)
                     /locus_tag="QBH27_001840"
     CDS             complement(13374..14660)
                     /locus_tag="QBH27_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ7014161.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFNGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14711..15652)
                     /locus_tag="QBH27_001841"
     CDS             complement(14711..15652)
                     /locus_tag="QBH27_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDQ7014162.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15667..16437)
                     /locus_tag="QBH27_001842"
     CDS             complement(15667..16437)
                     /locus_tag="QBH27_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDQ7014163.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            16910..18424
                     /gene="caiT"
                     /locus_tag="QBH27_001843"
     CDS             16910..18424
                     /gene="caiT"
                     /locus_tag="QBH27_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDQ7014164.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKY"
     gene            18455..19597
                     /gene="caiA"
                     /locus_tag="QBH27_001844"
     CDS             18455..19597
                     /gene="caiA"
                     /locus_tag="QBH27_001844"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDQ7014165.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            19726..20943
                     /gene="caiB"
                     /locus_tag="QBH27_001845"
     CDS             19726..20943
                     /gene="caiB"
                     /locus_tag="QBH27_001845"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDQ7014166.1"
                     /translation="MDHLTMPKFGPLSGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            21016..22569
                     /gene="caiC"
                     /locus_tag="QBH27_001846"
     CDS             21016..22569
                     /gene="caiC"
                     /locus_tag="QBH27_001846"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDQ7014167.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDIYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSVRAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSVNDRQHCLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            22678..23463
                     /gene="caiD"
                     /locus_tag="QBH27_001847"
     CDS             22678..23463
                     /gene="caiD"
                     /locus_tag="QBH27_001847"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDQ7014168.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            23469..24059
                     /gene="caiE"
                     /locus_tag="QBH27_001848"
     CDS             23469..24059
                     /gene="caiE"
                     /locus_tag="QBH27_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDQ7014169.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVLAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(24178..24573)
                     /gene="caiF"
                     /locus_tag="QBH27_001849"
     CDS             complement(24178..24573)
                     /gene="caiF"
                     /locus_tag="QBH27_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDQ7014170.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(24834..28055)
                     /gene="carB"
                     /locus_tag="QBH27_001850"
     CDS             complement(24834..28055)
                     /gene="carB"
                     /locus_tag="QBH27_001850"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDQ7014171.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(28073..29221)
                     /gene="carA"
                     /locus_tag="QBH27_001851"
     CDS             complement(28073..29221)
                     /gene="carA"
                     /locus_tag="QBH27_001851"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDQ7014172.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
     gene            complement(29677..30498)
                     /gene="dapB"
                     /locus_tag="QBH27_001852"
     CDS             complement(29677..30498)
                     /gene="dapB"
                     /locus_tag="QBH27_001852"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDQ7014173.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(30659..31024)
                     /locus_tag="QBH27_001853"
     CDS             complement(30659..31024)
                     /locus_tag="QBH27_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ7014174.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(31047..31961)
                     /gene="rihC"
                     /locus_tag="QBH27_001854"
     CDS             complement(31047..31961)
                     /gene="rihC"
                     /locus_tag="QBH27_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDQ7014175.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYNFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(32027..32977)
                     /gene="ispH"
                     /locus_tag="QBH27_001855"
                     /gene_synonym="lytB"
     CDS             complement(32027..32977)
                     /gene="ispH"
                     /locus_tag="QBH27_001855"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDQ7014176.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(32979..33428)
                     /gene="fkpB"
                     /locus_tag="QBH27_001856"
     CDS             complement(32979..33428)
                     /gene="fkpB"
                     /locus_tag="QBH27_001856"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ7014177.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(33536..34030)
                     /gene="lspA"
                     /locus_tag="QBH27_001857"
     CDS             complement(33536..34030)
                     /gene="lspA"
                     /locus_tag="QBH27_001857"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDQ7014178.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKAAQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(34030..36846)
                     /gene="ileS"
                     /locus_tag="QBH27_001858"
     CDS             complement(34030..36846)
                     /gene="ileS"
                     /locus_tag="QBH27_001858"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDQ7014179.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(36889..37830)
                     /gene="ribF"
                     /locus_tag="QBH27_001859"
     CDS             complement(36889..37830)
                     /gene="ribF"
                     /locus_tag="QBH27_001859"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDQ7014180.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(37838..38056)
                     /locus_tag="QBH27_001860"
     CDS             complement(37838..38056)
                     /locus_tag="QBH27_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDQ7014181.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAVGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            38159..>38391
                     /gene="rpsT"
                     /locus_tag="QBH27_001861"
     CDS             38159..>38391
                     /gene="rpsT"
                     /locus_tag="QBH27_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDQ7014182.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKA"
BASE COUNT         9337 a        10479 c         9706 g         8869 t
ORIGIN      
        1 ggcaattgtg cgccgattgt ctgatgctgc tcgccggatg cgacgctggc gcgtcttatc
       61 cggcctaccg cgtcttatca ggcctacacg ttgaaactta tttaaaccct ttctgctgct
      121 ttatcagctc ataggcctgc tgaatttcct gcgctttttg cttcgccatc tccatcatct
      181 caggcggcaa acctttcgcc accaacttat cgggatggtg ttcactcatc agcttacggt
      241 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
      301 cttccagcgt cgggccacgc tgcgcttgct gccagttacc actgccagat tgctgctgat
      361 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
      421 gggagatgcc taactcttcg gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
      481 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
      541 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
      601 ttcccacccg gaacgcattt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
      661 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
      721 aatggcccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
      781 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacagaccta
      841 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
      901 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
      961 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     1021 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     1081 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     1141 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     1201 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     1261 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     1321 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     1381 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     1441 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     1501 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     1561 gataccaacg tctgggaagg tgattaccag atggtgggtc gccaggggcg cggtaaagcg
     1621 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     1681 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     1741 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     1801 tatagcccgt atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     1861 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     1921 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     1981 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     2041 ctgccttcag ataaagtcta taaagatgaa cacccgaacg atgacagttc acgtcgttgg
     2101 ttgttctact ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac
     2161 accaaagtca gcgatcctag ctacttcaat gacttcgata acaaatacgg ttccagtacc
     2221 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     2281 gtttcaacca agcagttcca ggtcttcagc gagcagaaca ccagtagcta ctcggcagag
     2341 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     2401 ggtcaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     2461 ctggaaccga ccatcaatct gccgctctct aataactggg gcagcatcaa taccgaagcg
     2521 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     2581 accaggctgg cagagtccgc taaccgtgta atgccgcagt tcaaggtaga cggtagaatg
     2641 gtcttcgagc gtgatatgga aatgctggcg ccgggctata cccagacact ggaaccacgc
     2701 gcgcagtatc tgtatgtgcc atatcgcgat cagagcaaaa tctataacta cgactcttcg
     2761 ctgctgcaat ctgactactc cggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     2821 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     2881 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     2941 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtatg ggcaggcgat
     3001 acttactggc gcatctccga tcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     3061 ctggataacg tagcgaccag taactccagc attgaatacc gtcgtgatga agaccgtctg
     3121 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
     3181 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgccgt cgccagctgg
     3241 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
     3301 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcgat tcgcgtcggt
     3361 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
     3421 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
     3481 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
     3541 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
     3601 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
     3661 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
     3721 aaaactgaac gctgctcagg caaggcaaca acttcctgat gacgcgacgc tgcgccacca
     3781 aatcatggaa cgtttgatca tggatcaaat tatcctgcag atggggcaga aaatgggagt
     3841 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
     3901 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
     3961 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
     4021 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caagtgggta accaaaacga
     4081 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc
     4141 tgatcaggtg aacgaagcgg aaagccaggc gcgcgcgatt gtcgatcagg cgcgtaacgg
     4201 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
     4261 ccagatgggc tggggccgta ttcaggagtt gccggggatc ttcgcccagg cattaagcac
     4321 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
     4381 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
     4441 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga aactggaaca
     4501 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
     4561 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
     4621 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
     4681 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
     4741 cgacgcggca cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
     4801 agcagcaagc tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta
     4861 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
     4921 ttaattgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
     4981 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
     5041 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
     5101 cgtgaatctg tcactgtggg gctgttagcg gttgaaaatg ggcactatgt ggtggaaacg
     5161 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
     5221 gtgcataaag gcgtcattaa cgacgcaggc attccgttta ccggtcatac cgagtttttc
     5281 gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
     5341 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgccctt
     5401 ttgcacgaag tgattgctat tttgcatcac gatttgcgga ccaaatttgg tattgccgaa
     5461 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
     5521 gaagagatag acaccattat tccggtactc gacgagctgc gggaacaggg gatgaaactc
     5581 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
     5641 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
     5701 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
     5761 cttgaactgg cgggacgtgg taaagccgat gtcggcagtt ttattacggc gcttaatctc
     5821 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
     5881 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
     5941 ccattaaccc gcagaagggc caggcgatgg tcgaaatcgg ccccggtctg gcggcattga
     6001 ccgaaccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
     6061 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
     6121 cgatgacctt taactttggt gaactggccg cgaaaatggg ccagccgctg cgtgttttcg
     6181 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
     6241 ccattgccga catgcacttt atgttgcaaa aagaagtggt gaatcgtctg gttgcaggac
     6301 cgaacagcaa ggcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
     6361 cggtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
     6421 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
     6481 gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
     6541 tgtttagcgt cggggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
     6601 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
     6661 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
     6721 ggcccaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
     6781 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
     6841 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
     6901 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
     6961 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
     7021 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
     7081 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
     7141 agataccctc tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct
     7201 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggtaatc acgatctgca
     7261 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
     7321 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
     7381 aatcgacgaa gagaaaaagt tggtgatggc ccacgccggg atcacgccgc agtgggatct
     7441 gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc
     7501 cttcttcctt gatgccatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
     7561 gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa
     7621 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
     7681 gtggtttgcg attcctggcc ccgtcgctga agagtacaac atcgcctttg gtcactgggc
     7741 atcgctggaa ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg
     7801 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
     7861 ccggcataag gatttgagtg agggagaggc ggtagcgtct taaatcccgt cgagcataaa
     7921 atctaatgct catttaatca acgttcggtg cgcgctggca tcgcaaaatt tcgttcccag
     7981 cgcgttaact ggaacagttg cagtctcatg cgtcatgagc acatattctt tgccgtctat
     8041 cagtaaaagg attgggttaa gccgttctgg tgggcgaata cggctaaatc gttcaatagg
     8101 tgttaatgga ataacgatac ggcgattaat ctcaccaata atgtcgctgg taacatcaat
     8161 aacaaaggga aacgcggcgt tcctgccgcg actgcggtat accgtaaatt gcatcgctag
     8221 aagctccggt actcatcaga gaaacatccg gtttcatcag ccaattgatt taacgcagcg
     8281 atagcttctg cgttctcttc acgccaacgt gttgctgcat gttttttaag ttcagcattg
     8341 agcgcaaccg taagggtggc gctcatattt aagcctgcat cacgagcgcg attgactaac
     8401 tcacggtcga cggtgacggt cacactttgt gtggtacgtt ttgcagtcat gatctcgtgc
     8461 tcctgtacaa tttatacgtc tagtatacac ggatatctgc acgtatcagt gatgccggaa
     8521 ttctaatata cgaaattacc gccgctccag aatctcaaag caatagctgt gagagttctg
     8581 cgcatcggcg tcgtggaatt cactgaatac cgattcccag tcatccggct cgtaatccgg
     8641 gaaatgggtg tcgccttcca cttccgcgtc gatatgcgtc agatacagtt tttgcgcttt
     8701 cggcaggaac tgctcataaa cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc
     8761 acacgccgca atggcttcat ccaccgactt cacccacgtt acgcgatcgt ccgtacccgg
     8821 ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgatgg attcccaggt
     8881 atggcggccc ataatcacgg gtttatttaa agtgttgcgt ttaaaccagg cgagatcggc
     8941 aggcaggttc cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc
     9001 aatcagactg atcattgaga tttcccgata aaaaaaattg tcgccactat acgtaaagcg
     9061 taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga
     9121 gtccacgctc tctccctgga ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg
     9181 taaagcgtga tttacgtcac tctttattag gatgagggct tcgtttccgg ttcatccgcc
     9241 atattgccag tatgtttccc ttcttcggtt ccctgccagc catgtcgttg aattaatgac
     9301 agatgttcac ggtcttcggt aatgatctca cttaacatcg cgctggtgcg tttatagacc
     9361 gccgcacggg ctttggtgtc gttctcaacc attgccatct cttccaccat ctgaatatta
     9421 aagcggcgga acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc
     9481 agtgccagac gcccggtttt cagcgcacct tcgaaggttt cacgctccgg cttttcaacg
     9541 cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat aatctgcaaa
     9601 tgtgggaaat gttctttcac catctccgtc agttgcaggt tggtttgcgg atcgtcgatg
     9661 gcgttaatca gcacttccgc tttcgccgct ccggcagatt ccagtaaatc catccgcgtg
     9721 gcatcgccat aaaacacttt cataccgaat ttacgcagcg tttcgatatg gtccgggtcg
     9781 tgatcgagca ccaccatttt caccccgctg gagagcagta aacgtccggt tatctgccca
     9841 aaacgaccga atccggcaat gattacccgc ggctgttctt cgtcgatctc atcggcttca
     9901 cgcgcctctt cagtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt
     9961 gccgccatcg acagtgccac cgccagggtc agcgatttcg cccactctgg ctccagcaca
    10021 ttcgccatct gcgccgcacc aaatactaca aaggcaaact cactgccctg ccctaacagc
    10081 accgcaaacc agcgacgctg tttatttggc acctgcaacg gccgggcaat cagccacagc
    10141 atggcgattt tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa cggattttca
    10201 agcagcgtgc caaagtctat cgacatgcca acgccgatga aaaacagccc cagcagcaaa
    10261 cctttaaatg gttcgatatc gctttccagc gcatgacggt attccgagct tgccagcagt
    10321 acgcccgcca gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca
    10381 aagccaaaca cgaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggca
    10441 acaaaacgca gtgccggacg cgtgacatag cgccccagca ataccaccag caccagcgcg
    10501 cccgccactt ttaacgccga gagaacaaat gcgcccatcg tcgtcgaggc actactcgct
    10561 gccagtagcg ggatcatcgc caccagtggg atcgccgcga tatcctggaa cagcagcacc
    10621 gcaaaggctc tgcggcccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg
    10681 gcaatcgccg ttgatgagag cgccagcgtc atgccgatca attctgcgac ctgccagcgc
    10741 agcccaagta acatacaaaa cagccccagc aggccgccgc aaatcaccat ctgcaatgcg
    10801 ccaccgccga acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg
    10861 ataataaaca gcatcagcac cacgccaatc tctgcaaagt gcagaataga ttcggcatcg
    10921 gtcaccagtc gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagaacc
    10981 gatcccagac cgagacgtac cgcaatggga acaatcagcg ccgccgaacc gagataaatc
    11041 agcgcctgaa taagcgtatg gctatccatg atgtgcctcc tgccattcca gcagacgttg
    11101 cttatagtgc cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat
    11161 ggcaaacggt ggcagccagt tcagcccgca gtagattgcc gtcgcctgta gcggctgcga
    11221 cagcacatca aagcccggat gcgcaccaat ctcaaaatgg ctttccccgc cgccggtcgt
    11281 caccgcccac agcaaatgtt tgccgtgcag cgccgtgccg ccgtgaccgt aagcccagcc
    11341 gtgcgagaaa actttatcga tccaaagttt aagaagcggt ggaatgctgt accactgcat
    11401 cgggtgctgc cagacgatca gatcggcacg agacagcgcc tcctgttcgg cggcaatgtc
    11461 gatattgaag tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct
    11521 tgcctgttca agcatccgtt tattcgcatg ggaatgatgc ggatatggat gcgcataaat
    11581 tataagaatc ataggtagcc cgcgtgatta cgcctttgtt ttcccacaaa gtgtagtcat
    11641 atattcacgc agcttatagt caataatatt gattcagaaa agagagaaat ctgaacaaat
    11701 taccccattc tctgcccgcg gatcgtcata ttgctggtct gcgccagtga catccctcga
    11761 gtctccgggg caaacgctac ggaaatcaac aagccaaata gcgagatacc cgcccccatt
    11821 agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgtcca ggtcgataca
    11881 atagtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg gatatccgtc
    11941 gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca
    12001 tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag ccccaaaacc
    12061 gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    12121 tttagccaca gcatcggcgg aatacagccg agcataaaga acaggctaat caccacgttc
    12181 cccagtgccg cgtttttgcc aacccccaat cccaacaaac caacgatttg cgggccaaag
    12241 gtgtaaatgg cgaacatggg gatcacctgg caggtccaga tagcggcaac aaacagaaca
    12301 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttcc
    12361 tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc ttttactcgc
    12421 cctttgcgta acaaccagcg gggagattca ggcagttcga atcgaccaat caaaatcaac
    12481 aaacagggaa tcgctgcgct acccagcatc cagcgccagc cgccttccac atcataaagc
    12541 caatagccga ccaaatcggc gcaggtcgcg ccgacatacc acatcgcggc aataaagctg
    12601 atggaaaacg cccgctgacg ggtactggag aactcggtga tcattgaggt ggcgatggga
    12661 taatccgcac caatgacaat gccgataagt acccgcatca ccaacagttc gacgggggat
    12721 gagacaaaca tcgttgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt
    12781 ttgcgccgtc cgactttatc ggagatatag ccaaacagcg atgtgccaac gaacagcccg
    12841 gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt ccagtttcag cgccggagtc
    12901 agttgctcca gcgccacgcc aatcattacc agtacataac catccagaaa cggaccaccg
    12961 cttccccaca gcaaaatgcg gcggtgaata gaggagaatt taagatcatc aaagtttctg
    13021 gacggttgca tgggttattc ctgttttttt gcaatgagag tcagtacggc attacgtatc
    13081 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    13141 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    13201 atcgaagcgc acactgccgt catcccgctt cttgtacaga cctgcggggc acgctttcac
    13261 cagcagctcc agggcctgtt tatcggcatc ggccttcaca acaatgtgcg gatgctcttc
    13321 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    13381 acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt cttgccgtgg
    13441 cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa caggtcacgc
    13501 gccacgccca ccgccagttc cgggtagccg ttaaacatgc gtgggttatc aaggaacgcc
    13561 ggtagtttct ggtacatgcg catatcgcgc agcgggccac tctcaagatg ctgacgatat
    13621 tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac agtttttgct
    13681 gctgcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    13741 cacattccgg cagcatcacc ggcaatcaat acgccgtcac cgaccagctc cggcagcatg
    13801 ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc gcccgcgatc
    13861 agcggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac cgattttttt
    13921 gcgtcatgca gatgatggag accacaaacc agccccagcg acagggtatt ttcattggta
    13981 taaaggaagc caccgcccat caggccatcg gtgggtgatc ccgcaaacag gcaagccgcg
    14041 ccctgattac cctgcaactg aaaacggtct tcaataaccg acttcggtaa ctcgatcagt
    14101 tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc cagcttttcg
    14161 gcaaggatgg aattcacccc atcagcaagg atcaccgttt tcgcttcaat cacatcgcca
    14221 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg
    14281 gtaattaact gcgcgcccgc ttcttcggcc tgctccatca gccaggcatc aaatttactg
    14341 cgcaaaacgg agtaagaacg ctgggatgga gaggtttcgt cgccattgca gtagtccata
    14401 gtcatcgctg acttttccgt cataaacgca agtttttcat gggtgatcag gcgttctacg
    14461 ggggcggagt cagcgaaacc aggaataatg tgttccagac tgtgggcata gagacgcccg
    14521 ccagtgacgt tcttggcacc tgcggaattg cctcgctcga taactaacac ttgcgcacct
    14581 tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg
    14641 tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccggaa tgcagccctg
    14701 ccagagtgga tcaacgcgct aaagctgcgg tcagtgccgg aaggatcttc acggcgtcgc
    14761 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatctttg ttgatggcga
    14821 aaatggtttg cgacgcatta gcgccaacca tgtgctggat ctgcccggag atccccaccg
    14881 ccaggtacaa ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    14941 tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct atcgccttac
    15001 aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgaccg acgctgacca
    15061 ccaggcgggc tttgtcgaga tcgacgctgt tgctctgacg cgcctgggtt gccgtgcggg
    15121 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    15181 ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa
    15241 tgcgttcttc gccaatcgcc agaccgccgt aaaccatgtg tttcactgtc gctttaccgt
    15301 cctgcacgct aacagcgctg gcatcgttag acaccgccgc gttaaggcgg taacccagtt
    15361 tcgccgccag taatttgccg cgacgggtgt ttggcaacag caccagaccg tctgcgccgt
    15421 gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccgc tcgtctggtt
    15481 tgccgcttaa tttccagaca tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat
    15541 cattgaggac gaacgtgttg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    15601 gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    15661 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    15721 gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc gcggcaacct gttgttctga
    15781 ccaggctgcc tctgcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt
    15841 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    15901 agcaacaacc gcaggcagcg gaatgcttaa ggtttctgtt tcatcttcca gttcgcgctc
    15961 aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa ctgccggaat
    16021 attaaggatt tcgcctacca gcaaaccaac ctgctgggca taaaggtcgg aagaaccatc
    16081 gccacagagg atcaaatcaa agcctgcttt ctgagcggct gcagcaagta cgctcgccgt
    16141 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccggacc
    16201 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttaa
    16261 ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    16321 gagatcgtat tggcttattt tggcatcggc tttgctgaag tctaacgaac catcagcatt
    16381 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    16441 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcac
    16501 gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa
    16561 atccaacttt caatattgtt aagctcctca ccaatattga aaacatggcg tagcaaaaag
    16621 aaattttcaa tattgtttta tggatcacca atattgaaag tcagatgcta tttttttacc
    16681 cacaacagaa cataacaaac tgattaataa tcaaaattaa caaaaaaatg ccctctattc
    16741 acccgaaaca aaaatgtgac aacaatcaca gaatgcagct tattgaatac ccattatgag
    16801 ttagccatta acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa
    16861 acaaacggga acgcaaaata aatattcgtt ttcacagtgg aattaactca tgaagaatga
    16921 aaagagaaaa acgggaatag aaccgaaggt tttctttccg ccgttaataa tcgtcggcat
    16981 actttgttgg cttacagtca gagatctgga tgcagcgaat gtcgttatta atgctgtatt
    17041 cagttacgtc accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt
    17101 cggctggttc tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccacc
    17161 agaatttagt accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt
    17221 actgttctgg ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga
    17281 accgaactca acaggcgcga aagagttggg gctggcttac agcttgttcc actggggacc
    17341 tctgccgtgg gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg
    17401 caaaatggaa gtgattcgcc ccagctcgac cctggtgccg ctggtaggtg aaaaacacgc
    17461 caaagggttg ttcggcacta tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat
    17521 gggtaccagt ctgggccttg ccacgccgct ggtaaccgag tgtatgcaat ggttgtttgg
    17581 cattccgcat accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc
    17641 catttgcgtc gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta
    17701 cctgagcttc ctgatgctgg gttgggtgtt catcgtcagc ggtgccagct tcatcatgaa
    17761 ctacttcacc gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac
    17821 cgatcccatc gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg
    17881 ggtgatttac gctatccaga tgagtatctt cctcgcccgc atctcccgtg gtcgtaccgt
    17941 gcgtgaactg tgcttcggca tggtgctggg gctgacagcg tcaacctgga tcctgtggac
    18001 tgtactcggt agtaacaccc tgctgttgat agataaaaac atcatcaaca ttccaaatct
    18061 gatcgaacag tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc tgccactcag
    18121 caccgccacc atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa
    18181 cgcctgctct tataccctgg cgatgtccac ttgccgcgaa gtacgtgacg gcgaagaacc
    18241 cccgctgctg gtgcgcattg gttggtcagt tctggttggc attatcggca ttgttctgct
    18301 ggcgctcggc ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt
    18361 cttcgtcaac atcatggtga cgctctcctt tattaaagac gcgaaacaga actggaaata
    18421 ttaattaacc ccaaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca
    18481 ggaactgttt gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt
    18541 tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagccc tggcggatat
    18601 gggtatcgac agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac
    18661 tctcgccgcc gtgtggatgg aactgggtcg tctgggagcg ccaacttacg tgctgtatca
    18721 gttaccgggc gggtttaaca ccttcctgcg tgaaggtacg caagagcaga tcgacaagat
    18781 catggctttc cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc
    18841 aggctccgac gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct
    18901 taatggtagt aagtgtttta ttaccagcag cgcctacacc ccgtacatcg tggtgatggc
    18961 gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa
    19021 accgggcatc aaagtgacca aacttgagaa gctcggtctg cgtatggata gctgctgtga
    19081 aatcaccttt gacgatgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg
    19141 ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta
    19201 cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt
    19261 tggcgaggct attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa
    19321 attaaactcc atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat
    19381 cacctctggc gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt
    19441 ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg ggtaatcacc gcatcagccg
    19501 cttctggcgt gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa tgcagatcct
    19561 gacgctgggc cgcgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac
    19621 gctgacgcgt cttatcaggc ctacaggagc acgaatgtcg gccggataag gcgtttacgc
    19681 cgcatccggc agtcatgcgc tacataaatt tttcaggagc taataatgga tcatctaacc
    19741 atgccgaaat tcgggccgct gtccggattg cgcgttgtct tctccggtat cgaaatcgcc
    19801 ggaccgtttg ccggacaaat gttcgcagaa tggggcgcgg aagttatctg gatcgaaaac
    19861 gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc cgcaactttc ccgccgcaac
    19921 ctgcacgcgc tgtcgttaaa tatcttcaaa gatgaaggtc gtgaagcgtt tctgaaatta
    19981 atggaaacca ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc
    20041 attaccgatg aagtcctgtg gcagcacaac ccaaaactgg ttatcgctca cctgtccggt
    20101 tttggtcagt atggcaccga ggagtacacc aatcttccgg cctataacac tatcgcccag
    20161 gccttcagtg gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg
    20221 tataccgccg attacttttc tggcctgact gccaccacgg cggcgctggc agcactgcat
    20281 aaagtgcgtg aaaccggcaa aggcgaaagc atcgacatcg ccatgtatga agtgatgctg
    20341 cgtatgggcc agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg
    20401 accaaaggga aagatcccta ctacgccggt tgcggtctgt ataaatgcgc cgacggctac
    20461 atcgtgatgg aactggtggg cattactcaa attgcagagt gctttaaaga tattggcctc
    20521 gcacatctgc tcggcacgcc agaaattccg gaaggcaccc agcttatcca ccgtatcgaa
    20581 tgcccttacg gcccactggt agaagagaaa ctcgatgcct ggctggcggc acataccatc
    20641 gcagaagtta aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta
    20701 ccggaactgg aaagcaatcc acagtatgtg gctcgcgaat caatcactca gtggcaaacg
    20761 atggatggtc gcacctgcaa agggccgaat atcatgccga aattcaaaaa taaccccgga
    20821 caaatctggc gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc
    20881 ggctacagcg aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac
    20941 taaacgcgtg gcgtcagtca gcttgcctga ctagtgcccg ctcaataaca aacgaacaaa
    21001 atggatagag gtgcaatgga tatcattggc ggacaacatc tacgtcaaat gtgggacgat
    21061 ctggcggacg tttacggtca taaaacggcg ctgatttgtg aatccagcag cggagtcgtt
    21121 aaccggtata gttatcttga gttaaatcag gagattaacc gcacggcaaa cctgttttat
    21181 acgctgggga ttcgcaaagg cgacaaggtt gctctacatc tcgacaactg cccggaattt
    21241 atcttttgct ggttcgggtt ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc
    21301 ctgttgcgcg aagaaagcac gtggatcctg caaaatagcc aggcgtgcct gctggtgacc
    21361 agtgcgcaat tttatcctat gtatcaacag attcagcagg aagatgcctc tcagttgcgg
    21421 cacatttgcc tgacagatat ggcacttccc gctgatgatg gcgtgagttc gtttactcaa
    21481 ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc cgctatcgac tgacgatacg
    21541 gcggaaattc tcttcacctc cggcaccacc tcccgaccga aaggggtggt gataactcat
    21601 tacaacctgc gcttcgctgg atattactcc gcctggcagt gcgcactgcg tgatgatgac
    21661 atctacctga cggtaatgcc tgcatttcat atcgattgcc agtgtactgc ggcaatggcg
    21721 gcattttctg ccggggccac ctttgtgctg gtcgagaaat acagcgtccg cgccttctgg
    21781 gggcaggtac agaagtaccg tgccaccgtt accgaatgta ttccgatgat gatccgtacc
    21841 ctgatggtac aaccaccttc tgtgaatgac aggcaacact gcctgcggga agtgatgttt
    21901 tatctcaacc tgtcagagca ggaaaaagac gctttttgtg aacgcttcgg cgtccgcttg
    21961 ctgacgtctt acgggatgac ggaaaccatt gttggcatta tcggcgatcg ccctggcgat
    22021 aaacgacgct ggccgtcaat tggtcgggcg gggttttgct acgaagcgga gatccgcgac
    22081 gatcacaatc gcccgctccc ggcaggtgaa atcggtgaaa tctgtattaa aggcgtacca
    22141 gggaaaacca tcttcaaaga gtattttctc aacccgaaag ccactgcgaa agtgctggaa
    22201 gccgatggct ggctacatac cggcgatacc ggataccgcg acgaagaggg ctttttttat
    22261 ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag
    22321 ctcgaaaata tcatcgccac ccatcccaaa attcaggata tcgtggttgt gggtattaaa
    22381 gattcgattc gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg tgaaacattg
    22441 agcgaagagg aatttttccg cttctgcgaa caaaatatgg cgaaatttaa agtgccctct
    22501 tatctggaga tcagaaaaga tctgccacgt aattgctcgg ggaaaataat tagaaagaat
    22561 ctgaaataaa tgaaacggca gggaactacc ctgtcagcta ataatcacgc tctaaaacaa
    22621 tatgcatttt ttgcagggat gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg
    22681 agtgaatcat tacatctgac ccgcaatgga tcaattctgg aaattaccct tgatcgtcca
    22741 aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc
    22801 cgtgacgatc cgcaattacg tgtcgccatt attaccggtg ccggagagaa attcttttcc
    22861 gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac cggatgctga ctttggcccg
    22921 ggaggttttg cgggattaac cgaaattttc aatctcgaca aaccggtcat cgccgctgtg
    22981 aacggctatg cctttggcgg cggctttgaa ctggcgctgg cggcagattt tattgtttgt
    23041 gccgataacg ccagcttcgc cctaccggaa gccaaactgg gcatcgttcc tgacagcggc
    23101 ggcgtgctgc gtctgccgaa gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc
    23161 ggcagacgaa tgggcgcaga agaggcgctg cgttgggggg tagtcaaccg cgtggttagc
    23221 caggcggaac tgatggataa cgcccgcgaa ctggcgcagc agctggttaa cagcgctccg
    23281 ctggcgattg cggcgctgaa agagatctac cgcaccacca gcgaaatgcc ggtagaagaa
    23341 gcgtatcgct atattcgcag cggcgtgttg aaacactatc catcggttct gcattcggaa
    23401 gatgccattg aagggccgct ggcgtttgcc gagaagcgcg atccagtgtg gaaaggacgt
    23461 taacaaccgt gagctattac gcctttgagg ggttaattcc ggttgttcac ccgacggcgt
    23521 ttgtccatcc cagtgcagta ttgattggcg atgtgattgt gggagccggt gtctacatcg
    23581 gcccactcgc ctcactgcgt ggtgactacg ggcggttgat cgtgctagcg ggagccaata
    23641 ttcaggatgg ctgcattatg catggctact gcgacactga caccatcgtt ggggaaaacg
    23701 gtcatatcgg gcacggagcg atcctgcatg gttgtgtgat tggtcgcgat gcactggtcg
    23761 ggatgaacag cgtgattatg gatggcgcgg tcattggcga agagagcatt gttgccgcta
    23821 tgagctttgt caaagcgggc tttcgcggcg agaaacgcca gttgttgatg ggtacgccag
    23881 cccgcgctgt acgcagcgtg agtgacgacg agttacactg gaaacggttg aataccaaag
    23941 agtatcagga tcttgtcggg cgctgtcatg catcgttaca tgaaacgcag ccactgaggc
    24001 aaatggagga aaatcgcccc cgtttgcagg ggacgacgga tgtgacgccg aaacggtgat
    24061 cttgccggat gcggcgtaaa cgccttatcc gacctggctg gaaagcaacc gtaggccgga
    24121 taagatgcgc cagcatcgca tccggcaacc aggctgcgtg gtatcaacga aatgacatta
    24181 acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa gttccgtgag
    24241 cggaacggca ggaatacgcg gcgtctttct tacgccgact aatttatccc ggctgttatt
    24301 gcgcagacgc gcgtaaattt gctcatcgat atcaaccact ttaaccagtc gctgacactg
    24361 gcatccccgc ccttccagct tattagggat catcttaact tcgcagctta tttctgtgac
    24421 ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat cgaaatgaat
    24481 agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg cgattaacaa
    24541 gtagagtggt ttttcaacat atccttcaca catacgaact ccagtttcac aaattaaaag
    24601 actggctatg actcgttttg cagcaattaa acaaggccat attaaaattg tggatatatc
    24661 gatagtatgc aacttcaccg tcgataagtg tgagctaaat cctgcttatg tcatcaaaaa
    24721 tatcgattcc ccaaaagatt tacttaatgg gcaaaaaaaa cggtcctcat cagagaaccg
    24781 tattagttat ttgatcaaat tagcggatga aaaatatccg ccatgacacg ctattatttg
    24841 atctgcgcgt gcatttcctg caccgaaatt actttttcgg tcgcatcggc attcagcgcc
    24901 atcgcggtgg caaagccgcc gttcagggtg gtgtcgtaat gcactttata ttgcagcgca
    24961 ctgcgacgga tcacgcggga gtcttcaatc gcacgacgac ctgaggtggt gttgatgatg
    25021 taggtatatt cgccattctt aatacggtcc tgaatgtgcg gacggccttc atgcaccttg
    25081 ttcaccagac gcggattgat acccgcttcg cccagcacaa tcgccgtgcc gtgagtcgca
    25141 tccagctcga agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttatcg
    25201 ccttcgcgca cggaaagcag cgcacgaccg tgtttcttca tggtggagtt gctgcccagc
    25261 tgcgctttgg caaacgcttc agcgaaggtg cggcccacgc ccatgacttc cccggtagag
    25321 cgcatttctg gccctaacag cgggtcaacg cccgggaatt tattgaacgg cagcaccact
    25381 tctttcaccg agtagtacgg cgggataact tctttggtta cgccctgctc agccagcgat
    25441 ttgccagcca tcacacgcgc cgccactttt gccagcggta cgccggtggc tttggagacg
    25501 aacggaacgg tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc
    25561 accgcaaact gcacgttcat caggccgcgc acctgcagtt cgaaggccag tttctgcacc
    25621 tgctggcgca tcacatcctg aatttcctga cttaaggtgt aggctggcag tgaacatgcg
    25681 gagtcaccgg agtgcacacc cgcctgctca atatgctcca tgatgccgcc aatcagcacc
    25741 atttcgccgt cgcagatggc atccacgtca acttctactg cgtcatcaag gaagtggtcc
    25801 agcaacactg gcgcatcgtt agatacgctg accgctgtct ggaagtagcg acgcaggtca
    25861 gcttcgtcat agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgcaccacc
    25921 agcgggtagc caatctcttt cgctttctca accgccattt cgatggtggt aacggtggcg
    25981 ttcgcaggtt gtttcagttt cagacgctca accgcatgct ggaagcgttc acggtcttcc
    26041 gcacggtcga tggcatccgg gctggtaccg ataaccggta cgccagcagc ttccagcgcg
    26101 cgcgccagtt tcagcggggt ctgaccgccg tactggacga taacgccttt cggcttctca
    26161 atacgcacaa tttccagcac gtcttccagc gttaccggct cgaagtagag acggtcagag
    26221 gtgtcgtagt cggtggagac ggtttccggg ttacagttaa ccataatggt ttcataacca
    26281 tcttcgcgca gcgccagcga ggcgtgtaca cagcagtagt cgaattcgat accctgaccg
    26341 atacggttcg ggccgccgcc gagcaccatg attttttcac gatcggtgga cggattcgct
    26401 tcgcactctt cttcataagt ggagtacatg taagcggtgt cggtggcgaa ctctgccgca
    26461 caggtatcca cgcgcttata aaccgggtgc aagtcatact ggtcgcgcag cttgcggatt
    26521 tctgcttcac gcacgcccgc gagttttgcc agacgcgcat cggcaaagcc tttgcgtttc
    26581 agctggcgca ggaattcagc gttcaggcca gtgatgccca cttctgcgac tttctcttcc
    26641 agacgcacca gctcttcaat ctgtaccagg aaccagcggt caatgttggt caaattaaac
    26701 acgccgtcta cagacaggcc cgcgcggaag gcatcagcga tataccagat acgctctgcg
    26761 cctgcatctt tcagttcgcg gcggatttta gtcagcgctt ccgggtcgtc gagactcact
    26821 ttcggatcga agccagttgc gcccacttcc aggccgcgca gcgctttttg cagggattcc
    26881 tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg
    26941 tcgttagcac cggcgaattt ctcgaagttg aagcgaggaa ttttagtaac cacatagtcg
    27001 atggacggct cgaaggaggc cggagtacgc ccaccggtga tgtcgttcat cagctcgtcg
    27061 agggtgtaac ccaccgccag tttcgccgcc actttggcaa tcgggaaacc agtggctttc
    27121 gacgccagcg cagaggagcg agacacgcgt gggttcattt cgataacaat caggcgaccg
    27181 tttttcgggt tcaccgcaaa ctggacgttg gaaccaccgg tttcaacgcc gatttcacgc
    27241 agtaccgcca tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct
    27301 ggcgcgacag tgatggagtc accggtgtgg atgcccatcg catcaaagtt ttcgatagag
    27361 cagacgatga tacagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag
    27421 ccgatcagcg actcatcaat cagcagctct ttagtcggag aaagatccag accgcgggcg
    27481 caaatttctt caaactcttc gcggttataa gcgataccgc cgccgctacc gcccatggta
    27541 aaggatgggc gaataatgca cgggaagcca acttcagcgg caaccgccag cgcttcttcc
    27601 attgaatgag cgataccgga acgcgcagtt tccagaccga ttttcttcat cgctacgtcg
    27661 aaacggcggc ggtcttcggc tttatcaatc gcatcggcag tggcaccaat catggtgacg
    27721 ccgaactctt ccagcacgcc ctgacgctca agctccagcg cacagttcag cgccgtctgc
    27781 ccccccatgg ttggcagcac cgcgtccggg cgctcttttt caataatctt gcgtaccact
    27841 tcccagtgaa tcggctcgat gtaggttgca tcagccattt ccgggtcggt catgatggtc
    27901 gccgggttgg agttcaccag aatgacgcgg taaccctctt cacgcagggc tttacacgct
    27961 tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga
    28021 atcaggatac tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag
    28081 cggttttacg gtactgctca attaacgcga taaagtggtc aaacaacggc gcggcgtcgt
    28141 gtggaccagg gctggcttcc gggtgtccct ggaagctgaa cgccggttta tcggtacgat
    28201 gaatgccctg taacgtaccg tcgaacaggg atttatgtgt gacacgcagg tttgcaggta
    28261 atgttgcttc atccaccgca aaaccgtggt tctgggcggt gatcatcacc acatttttct
    28321 caacatcttt aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg
    28381 caccgctcgc cagcgccagc agctgatggc cgaggcagat gccgaatacc ggaatatcgg
    28441 tttcgaggaa tttctggatg gcggtaatgg cgtaatcgca cggggccggg tcgccaggac
    28501 cgttggagag gaagatgccg tctggattca ttttcagcac atcttccgca gaagtttgcg
    28561 ccggaacgat ggtcagacga cagcctctat ccaccagcat ccgcaggatg ttgcgcttgg
    28621 caccaaaatc ataagccacg acgtggtacg gcagctcgtc ttcttttttc gcttctggca
    28681 gaccgccggt cagcgtccag ctcccttgtg tccagctata gggttctgcg gtagtcactt
    28741 cttttgccag atccatgcca ttcaggccag ggaacgcgcg ggctttttct aacgccagcg
    28801 ccgcatccgg gttatcgcct gcgataatgc agccattctg tgcgcctttc tcgcgcagta
    28861 aacgcgtcag cttacgggta tcgatatcgg caatcgccac gatgttatgg cgcttcaggt
    28921 aagaagagag gtcttcggta ttacgaaagt tgctggcaat cagcggcagg tcgcgaatca
    28981 ccagaccttg ggcatgtacc tgagaagatt cttcatcggc gtcattagtg ccgacattgc
    29041 caatatgggg ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt
    29101 cttgataacc ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa cctgttgccc
    29161 ctatggcccg accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca
    29221 aaacaccctc cagagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt
    29281 aaatcaatgc aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat
    29341 gggagctaaa agtcaactta atggcgggat tttttatatt attttgtggc tcatgcatgt
    29401 tttacaggca ttaagcgaca aaaagatcat ataacatgtt ctggaaaacg cttgcgcagc
    29461 aagaagattg catgaaatca aaatgagtag aaaaaagtgg accaaatggt caaaatttac
    29521 attaaaacaa caaaaacaga atacaaaaca tgtaactttc agcatttgca ttaaatttaa
    29581 aaacataaca ccaaaaataa aagggccaaa aattaaagcc ctttatattc aatgaattaa
    29641 atgtgttatt tttgcatcat tataaatatt ttgtggttac agattattga gatcaagcac
    29701 atctcgcata tcaaaaagac cactttcctt accactcaac cacaaagccg atcttaccgc
    29761 accgttagca aatgtcatgc ggctggatgc cttatgggtg atctccagac gctcgccaat
    29821 atcggcaaac atcgcggtat gttcgccaac gatgtcacct gcacgcacgg tggcaaaacc
    29881 aatggtgcca ggcacacgtt caccggtatg gccttcacga ctgtagaccg cgcaatcttt
    29941 cagatcttta tcaagagcgt gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg
    30001 cgcatcaact ttatgtctat gatgtgcttc aataatttcg atatcggtgt agtcccccat
    30061 cactttggct gctttctcca gcagtttaag catgacgtta acgccaacgc taaaattggc
    30121 ggcaaagaca atcgcaatat cggcggcggc gtcacgaatc gcctgtttac cggcttcgtc
    30181 aaaccccgta gtgccgatca ccatcccttt gccatgctgg cgacaaaaag cgagatggtt
    30241 cagcgtacct tccggacggg taaaatcgat aaacacatca aaatcatctt ttatcgcatc
    30301 aagactactt tgcacagtaa cgcctgtttt cccggctccg gccagttcgc ccgcatcgct
    30361 gcccagtaaa gaagatcctt cgcgctccag cgccgcgccc agctgcacgc cttctaatgc
    30421 cagcgccgct tgaatcaact ggcggcccat acggcccccg gctcccgcga tggcaacgcg
    30481 gatgtttgca tcatgcatag ctattctctt ttgttaattt gcatagaccg ttttcagagt
    30541 aaccagcgca tcgctacgcc gccagccaaa aacgctgata attagaaatt aatgataaac
    30601 agggagggat atcagtgaaa ggcatgacgc agaaagtgat gcgtcatacc ctctggcatt
    30661 atgcctggtc ttgtgacttt gacggcaggc agcacagtat aagcagaatt acgcccgtaa
    30721 cagggatcaa cacgacaatg gtaacccacc accctgaata tccgatatcg tgcatccttc
    30781 tgacggctaa cgctatcgcc ggtacggcaa aaatcaacgt taaaataaat gacgcgggca
    30841 gcgcaaaaaa aatgaaaaca ccatagtcat tgttaattgt taccgctaac caccaacata
    30901 aaagcggaag cagacagata acgccactac caatcataaa cgaccagaac tcgaagcgat
    30961 tggaaacacc ttcataatta aagatgtttt tccacccttc cagatacgct tctccatacg
    31021 tcatagaaca cttccttgtt ttattattag agcgccaggg ccagcacctc agccacccac
    31081 tgctggaagc ctttcacatc cagatccagc gccacctgta cattggctgg tttgcccagg
    31141 caaccgtcga tatcaacaac cgtcgtgcct gaggtaaatt caccctgagt ttctaccgcg
    31201 acaaaacacg gtttgagtgt gaacaattcc gggcgcacca gccaggcgat agcgcaaaga
    31261 tcgtgcatac gtagtccact ttgcatactg ccgctacggt agtggctaaa cagggcgtgt
    31321 agcatttttc cggtgcgatt caactccggc aaagtagcga gatagtcagg ggttaatatt
    31381 gcctgattgg tgacatccag accgcacatg acgatttcga tgccgctacg gaagacacag
    31441 gctgccgctt ctggatcggc agcgatatta aactcggcgt ttggtgtgca gttgccgcga
    31501 ccagcagaac cgcccatgat caccagacgg cgaatatacg gcttacattc cgggcattgt
    31561 gaaagtaaca gcgcaatatt ggttaacgga ccgatagcca ccagggtaac gggctctggc
    31621 gcacgcatca gggcatcacg aattgccaga aacgccggta tacccagtgg ctgacggttg
    31681 tgttcaacaa agttatagcc agccattccc gattcaccgt gtacagatgc cgcatcgcgc
    31741 ggcgcacgaa ccagtggtac ggcagcgccc tgggcaagcg gaatatccac attccagaaa
    31801 tgcagcagtt gcagggcgtt gcgggtggtt ttctctaccg aaacattacc cgcgactgtg
    31861 gtcatcagtt gcagatcgag ttcgggggta aaaatcgcgg cggcaatggc aacggcatcg
    31921 tcaatgccgg ggtctgtatc gaggaagata ggtaaatgca tgttttctcc ataaaaaatg
    31981 ccggtaacaa gaccggcatt ttcgcataac ttagactact aatgacttaa tcgacttcac
    32041 gaatatcgac acgcagctct ttcggcactt cgaagacgat attttcttcg cggccttcca
    32101 gcggaatggc ttcgccaccg ccaagctgct gcaagcgtgc caccacattc tgcaccagaa
    32161 tatccggagc cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt
    32221 cctggatatc tttcgcatcg tcaatcaaaa atgcgcgttt gcccatacgc tgggccagtt
    32281 ccgccagacg gttggagttg gaggagtttt tcgaaccaac caccagtact acttccgcct
    32341 gctctgccag ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt
    32401 tgcgcggacc gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt
    32461 catccaccga cagcgtggtt tgggtcataa aggagagctt ctcttcattt ttgaccgtca
    32521 gtttccacac atcgtctggc gattcgacca gatacattcc cccttccggg ttgctgtact
    32581 ggcccatcgt cccttccact tccgggtgcc cggcgtgacc gatgagaata gattcttcac
    32641 cacgacggct ggcgcgggcg acttccatat gcactttggt caccagcgga caggtggcat
    32701 caaacaccgt caaatcgcgg cttttcgctt cgttacgtac tgcctgagaa acaccgtgtg
    32761 cggagaaaat caggatcgcg ccgtccggta cttcgctaat ctgctcaata aagatagccc
    32821 cacgctcgcg caggctatcg accacgtagc ggttatgcac cacttcgtga cggacatata
    32881 tcggtgcgcc gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg
    32941 cacaaaaacc acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg
    33001 atttccagca cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca
    33061 acggtaatgg agtcgccgtt aatttcgcgg atcacgccag gcatctcact gccatccatt
    33121 gcggtaaaaa gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg
    33181 gagaagtact gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag
    33241 aaggtggttt tatcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga
    33301 gaagcatcac ccaggcggaa cagcgccggt ttaccgttgt tgcgggtaga ctcagcggtg
    33361 gtgccatcat cgagtttcag cgtgaagtgc accaggacgg cgctattgct ctgtacagat
    33421 tcagacatgc aggtttgctc ttatattttg aggatttttg ccggatgcgg cctacgattt
    33481 cgcagcaatc tgtaggccgg ataagatgcg tcagcatcgc atccggcagg gtttattatt
    33541 gttttttcgc tttagaaggt aaaaaacctt ccagcacaat cagtgccgca ccgacacaaa
    33601 tggcagtatc ggcaaggttg aaggtggcga agtgccagtc gccgacgtag aagtcgatca
    33661 tatcgacaac gaagccgtgc cacaggcggt cgaacaggtt gcccagcgcg ccgccaataa
    33721 tcagcgcgta agcgatattg tttagcttct gcgcggcctt cgagcgatac atcatcacta
    33781 ccaggatcac gctaatacca atcgcaatac cggcaaagaa ccaacgctgc cagccgccgc
    33841 tatcggcaag gaaactaaac gccgcgccat agttacgcgc ataatgcaga ttaagcgacg
    33901 ggaacagcgg gaccgtatcc cccagagcaa agttctggag gatcaggtat ttgctgccca
    33961 gatcgataat cagcacgact accaccagcc acagccagcg tagccctgtt gaacagatcg
    34021 attgactcat caggcaaact tacgtttttc accgtcaccg gcgacgttgc tgacacagcg
    34081 gccgcagatt tctgcgtgtt ccgccacctt gccgacatcc tgggtgtagt gccagcagcg
    34141 agggcacttc tcaccttcgg ctttactcaa cgcgactttc agccctttga ggacttcgct
    34201 ctgctgggca tcagcaggtg cgtcgttata gtctgcaacg gtagcgccgg aggtcaacag
    34261 gacaaatcgt aattcatcgc ccagcgcggt cagtttcgcc gccagttccg gttctgcata
    34321 caaggttact gcagcttcca gcgagccgcc cactttctta tcggcacgcg cttgctcaat
    34381 gactttgttc acttcgccac gcactttcaa cagctcgtcc cagaacgcat cgttcattgc
    34441 ttcactgtct gccagaccaa acaggccttc gtaccactcg ccggtgaaga cgtatttttc
    34501 acgttcgccc ggcaggtagc cccacacttc atcagcggtg aaggagagga ttggtgccat
    34561 ccagcgaacc agcgcttctg cgatgtgata cagcgcagtc tggcagctac gacgcgccac
    34621 gctgtccgct ttggcggtat actgacggtc tttgatgatg tcgaggtaga aggaacccat
    34681 ctcaacggag cagaagcgca tcagacgctg taccacttcg tggaaatcgt atgcttcgta
    34741 cgccttgagg atgtcttcct gtgccgcttt cgcacaacct acggcccagc gatccagtac
    34801 caccatctct tccggtttca ccatatcttt tgccggatca aaaccgttca ggtttgccag
    34861 caggaagcgc gcggtgttac ggatacgacg atagctgtcg gcagcacgtt tcagaatctc
    34921 gtcagaaaca gccatttcgc cggtgtagtc ggttgatgcc acccacagac gcagaatatc
    34981 cgcacccagt ttgttcatca catcctgcgg cgaaacggtg ttaccgatgg atttagacat
    35041 cttgcgaccc tgaccatcca cggtaaagcc gtgagtcagt acctgacgat atggcgcttt
    35101 gcccttcatc gcggtagaga tcatcaggga agacatgaac cagccacggt gttggtcaga
    35161 accttccaga tacatgtccg ctgcgtgacc ggcaaattcc ggacgcacat caacaacgga
    35221 agagtgggta gatccggagt caaaccatac atccagcgta tccggtactt tcacatactg
    35281 gtcagcttcg tcgccgagga tctctttcgc atcgagatcc caccacgcct ggatgccgtc
    35341 aacttcaacg cgttttgcca cttcttccat cagttcgaga gtacgcggat gcagttcttc
    35401 tgtgtctttg tgcacgaaca gtgacatcgg cacgccccag gtacgctgac gagagataca
    35461 ccagtcagga cggttagcaa ccatcgactc gatacgcgcc tggccccagt cagggatcca
    35521 ctgcacgcct ttgatctctt tcagtgactg cgcacgcaga cctttctgat ccatgctgac
    35581 gaaccactgc ggcgtcgcgc ggaagatgat cggcgtttta tgacgccagc agcacggata
    35641 gctgtgttgc attttctcaa cgtgcaacag tgcgcctttt tcctgcaaca acgcaatgac
    35701 aatatcgttc gctttgaaga cgttaacgcc atccagagtc gggtaagtac ccggcagata
    35761 agtgccgtcc gggccaaccg ggttagcggt ttccagacca tatttttgac cgatcacata
    35821 gtcgtccgga ccgtggcctg gcgcggtatg aacggcaccg gtaccggcat ccagcgtaac
    35881 gtggtcgcca agaattgccg gaacatcgaa gtccataaac ggatgggtaa agcgcaacag
    35941 ttccagctca gcacctttca ccgtgccaag aatggtgtaa tcgctaacgc cgatacgctg
    36001 cattacgctt tcaaccagat ctttcgccag aatcacggcc tgaccgtcga tttgcaccag
    36061 cgcataatca aaatcaggtg caatggagat tgcgcggtta gcaggcagcg tccacggcgt
    36121 ggtggtccat ataaccagcg aaattgggcc gttaacattg cttacgccaa atttcgtttt
    36181 cagcgcatcc tgatcgaccg cctggaaagc gacgtcgatg gacggagaag ttttgtcgta
    36241 atactcaact tccgcttctg ccagtgcaga acggcagtca acgcaccagt gcaccggctt
    36301 cgcgcctttg tgcaggtgac cgttgccgat gattttgccc agcgcgcgga tgatgttggc
    36361 ttcagttttg aagtccatgg tcaggtacgg gtgcgaccag tcgcccagca cgcccagacg
    36421 gataaagtct ttgcgctgac cgtcaacctg ggtcgcagcg tattcgcggc acttggcgcg
    36481 gaactcagcg gcggtgaatt tctcccccgg cttaccgtat tcttgctcta ctttcagttc
    36541 gattggcaga ccatgacagt cccagccagg cacatacggc gagtcatatc cagaaagccc
    36601 tttggactta atgataatgt ctttcagaat cttgttaacc gagtgaccaa tatgaatgct
    36661 gccattcgca taaggagggc catcatgcag aatgaaggtt tttttgcctt ttttagctgc
    36721 acgaatgatg ccgtacagat catcatcagt ccaacgcgcc agcattcccg gttcgcgctt
    36781 ggcgagatcg ccacgcatcg ggaaccctgt ttccggcaaa ttcagggttg atttatagtc
    36841 actcatcaga ttctcggttc cgtatttcgg tttgattaca taacaggctt aagccggttt
    36901 tgttagccca aaaaattcgc gggcggttaa ttcatcacgc gcaatctgcg ctttcagttc
    36961 gtccagcgat gcaaatcgct gctcattgcg tattttttta cgcagcacta cttgtatatg
    37021 gcgaccataa aggtccattg caacatctaa caaatgcact tccagttgct ggcgaatacc
    37081 ggcaaccgtt gggcgtgttc cgatgtttgc aacgccgggt aacggctttt cgccaaggcc
    37141 caacacttct accgcataaa cccctttcac cggggaaacc tgacggcgta gcggtacatt
    37201 cgccgtcggg aaacctatag tgcgccctaa ttcatcaccg tggactacac gcccggagat
    37261 ggcaaacggg tgccccagta aactttctgc cagagccaga ttgtcatccg ccagcgcctg
    37321 acgcacggcg gtgctgctga tacgcacgcc accttcgcaa aaagtttgcg tgctggtgat
    37381 atcgaagccg tattccatac ccgctttctg taataacaag aaatcgcctt cacgaccagc
    37441 gccaaagcgg aaatcgtcac ctacggcaag aaattttacc cgcaagtgct tcaccagaag
    37501 atcactgatg aagttttgcg cggttaacgc cgcaaaacgc ctgtcaaaac gcacgcacag
    37561 cacgtaatca acgccacact ctgcaagata acgcagtttt tcccgcagcc gggtcagccg
    37621 tgccggggct ttatcagtag caaacagttc cagtggttga ggttcaaaaa gcatcaccat
    37681 caccggtaag ttgcgcttgc gcccttcttc ctgcaagccc tgtaacagcg cgcgatgacc
    37741 gcgatgcacg ccgtcgaaat taccaatagt cagcacacac ccttcttgcg gggcctggct
    37801 gagattatgt atgccgcgta tcagcttcat gtctggctca aaacagtgaa aatcgtccga
    37861 gtataccttg tacagcggtc aaggttaacc ggcgattgag taccgaaatc agaaagaagt
    37921 ggcttttcat tgctatggcg caaatcatgg gaagaaactg accgcctact gcaatttttc
    37981 tcgcggaaaa gctgtattca caccccgcaa gctggtagaa tcctgcgcca tcactacgta
    38041 acgagtgccg gcacattaac ggcgcttatt tgcacaaatc cattgacaaa agaaggctaa
    38101 aagggcatat tcctcggcct ttgaattgtc catatagaac acatttggga gttggacctt
    38161 ggctaatatc aaatcagcta agaagcgcgc cattcagtct gaaaaggctc gtaagcacaa
    38221 cgcaagccgt cgctctatga tgcgtacttt catcaagaaa gtatacgcag ctatcgaagc
    38281 tggcgacaaa gctgctgcac agaaagcatt taacgaaatg caaccaatcg tggaccgtca
    38341 ggctgctaaa ggtctgatcc acaaaaacaa agctgcacgt cataaggcta a
//
LOCUS       DAOFSV010000035        37831 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.035, whole genome shotgun sequence.
ACCESSION   DAOFSV010000035 DAOFSV010000000
VERSION     DAOFSV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.035"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..561)
                     /locus_tag="QBH27_001862"
     CDS             complement(<1..561)
                     /locus_tag="QBH27_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7014183.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAA"
     gene            697..2421
                     /gene="iucA"
                     /locus_tag="QBH27_001863"
     CDS             697..2421
                     /gene="iucA"
                     /locus_tag="QBH27_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDQ7014184.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            2422..3369
                     /gene="iucB"
                     /locus_tag="QBH27_001864"
     CDS             2422..3369
                     /gene="iucB"
                     /locus_tag="QBH27_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDQ7014185.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            3369..5111
                     /gene="iucC"
                     /locus_tag="QBH27_001865"
     CDS             3369..5111
                     /gene="iucC"
                     /locus_tag="QBH27_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDQ7014186.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            5108..6385
                     /gene="iucD"
                     /locus_tag="QBH27_001866"
     CDS             5108..6385
                     /gene="iucD"
                     /locus_tag="QBH27_001866"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDQ7014187.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            6467..8668
                     /gene="iutA"
                     /locus_tag="QBH27_001867"
     CDS             6467..8668
                     /gene="iutA"
                     /locus_tag="QBH27_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDQ7014188.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9219..9362)
                     /locus_tag="QBH27_001868"
     CDS             complement(9219..9362)
                     /locus_tag="QBH27_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014189.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHRCVIQTVSFVRHILCYS
                     MGL"
     gene            9612..13499
                     /gene="sat"
                     /locus_tag="QBH27_001869"
     CDS             9612..13499
                     /gene="sat"
                     /locus_tag="QBH27_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HDQ7014190.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDHKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            13771..14031
                     /locus_tag="QBH27_001870"
                     /pseudo
     CDS             13771..14031
                     /locus_tag="QBH27_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14096..15247
                     /locus_tag="QBH27_001871"
     CDS             14096..15247
                     /locus_tag="QBH27_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDQ7014191.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            15260..16150
                     /locus_tag="QBH27_001872"
                     /pseudo
     CDS             15260..16150
                     /locus_tag="QBH27_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            16195..16617
                     /locus_tag="QBH27_001873"
                     /pseudo
     CDS             16195..16617
                     /locus_tag="QBH27_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17033..18565
                     /locus_tag="QBH27_001874"
                     /pseudo
     CDS             17033..18565
                     /locus_tag="QBH27_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(18599..19108)
                     /locus_tag="QBH27_001875"
                     /pseudo
     CDS             complement(18599..19108)
                     /locus_tag="QBH27_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            19128..19450
                     /locus_tag="QBH27_001876"
                     /pseudo
     CDS             19128..19450
                     /locus_tag="QBH27_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(20028..20243)
                     /locus_tag="QBH27_001877"
     CDS             complement(20028..20243)
                     /locus_tag="QBH27_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014192.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALHAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(20247..20606)
                     /locus_tag="QBH27_001878"
     CDS             complement(20247..20606)
                     /locus_tag="QBH27_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014193.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHEV"
     gene            complement(20897..21139)
                     /locus_tag="QBH27_001879"
     CDS             complement(20897..21139)
                     /locus_tag="QBH27_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014194.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            21624..21783
                     /locus_tag="QBH27_001880"
                     /pseudo
     CDS             21624..21783
                     /locus_tag="QBH27_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(22001..24091)
                     /gene="iha"
                     /locus_tag="QBH27_001881"
     CDS             complement(22001..24091)
                     /gene="iha"
                     /locus_tag="QBH27_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HDQ7014195.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASAQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTVGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(24591..25819)
                     /locus_tag="QBH27_001882"
     CDS             complement(join(24591..25504,25504..25819))
                     /locus_tag="QBH27_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7014196.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVIPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSFVSRCLRVSRAQLHVILRRADDWKDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQTELDGMPAINAKRVYRTMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGSELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQWASDGLSDNRCLEI"
     gene            complement(25971..26957)
                     /locus_tag="QBH27_001883"
     CDS             complement(25971..26957)
                     /locus_tag="QBH27_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014197.1"
                     /translation="MYHHDFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            27057..27791
                     /gene="nanR"
                     /locus_tag="QBH27_001884"
     CDS             27057..27791
                     /gene="nanR"
                     /locus_tag="QBH27_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDQ7014198.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(27827..28762)
                     /locus_tag="QBH27_001885"
     CDS             complement(27827..28762)
                     /locus_tag="QBH27_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7014199.1"
                     /translation="MGFIMNKDRSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQ
                     DPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPE
                     FLSQGYTLFVLSYRLPSPVEGENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFS
                     AGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLG
                     DNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHL
                     YEKGGHGFSIQWATDLSVGGWIKLFMDWMYSHGFK"
     gene            complement(28812..29921)
                     /locus_tag="QBH27_001886"
     CDS             complement(28812..29921)
                     /locus_tag="QBH27_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDQ7014200.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPADDYFLNNHIISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLP
                     WQGGMLILGGEKKDGKAVSDVIYLKKNDKQIKIVK"
     gene            complement(29934..30644)
                     /locus_tag="QBH27_001887"
     CDS             complement(29934..30644)
                     /locus_tag="QBH27_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HDQ7014201.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            complement(30690..31520)
                     /locus_tag="QBH27_001888"
     CDS             complement(30690..31520)
                     /locus_tag="QBH27_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7014202.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTGDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            complement(31524..32996)
                     /locus_tag="QBH27_001889"
     CDS             complement(31524..32996)
                     /locus_tag="QBH27_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7014203.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
     gene            complement(33045..33920)
                     /locus_tag="QBH27_001890"
     CDS             complement(33045..33920)
                     /locus_tag="QBH27_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDQ7014204.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(33954..34871)
                     /locus_tag="QBH27_001891"
     CDS             complement(33954..34871)
                     /locus_tag="QBH27_001891"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDQ7014205.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLFEEHDRNKKMKII"
     gene            35023..35160
                     /locus_tag="QBH27_001892"
     CDS             35023..35160
                     /locus_tag="QBH27_001892"
                     /inference="COORDINATES: protein motif:HMM:NF024407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDQ7014206.1"
                     /translation="MQCPVTILCVTLCDYLAPELITHSPEESDTVTLRKRRLPLEMMA
                     W"
     gene            35532..35957
                     /locus_tag="QBH27_001893"
     CDS             35532..35957
                     /locus_tag="QBH27_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDQ7014207.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            35954..36304
                     /gene="tnpB"
                     /locus_tag="QBH27_001894"
     CDS             35954..36304
                     /gene="tnpB"
                     /locus_tag="QBH27_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDQ7014208.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            36335..37432
                     /locus_tag="QBH27_001895"
                     /pseudo
     CDS             36335..37432
                     /locus_tag="QBH27_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            37432..>37831
                     /locus_tag="QBH27_001896"
     CDS             37432..>37831
                     /locus_tag="QBH27_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7014209.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGED"
BASE COUNT        10429 a         9229 c         8544 g         9629 t
ORIGIN      
        1 ggctgccatc agcagcccca gtggcgccag aggatgaatg gccagcattg ctgccgcgca
       61 cagcggcccg aatagcctgc cgcaactcag gccggagctg atggttgcca gagcggccat
      121 gcgattccct tctccagctc tctgcaatgc ccagacctga caggctggca ccattgccga
      181 cacggtcagc ccgtaggcga tccgggcgat gattaatatt cccagtccca ccgctgttgt
      241 tatggcgctg gtagccatca gcacgcttcc cagccctagc agggtaaagc ttatgccata
      301 tcctcccaga gcccacaaca ccacaggttt actgccagta cgggaaattt gctttcccca
      361 gcacggggac gatggcagaa acagcattga gccaagcatc agcagggcag cccatacaga
      421 cagactcaga tttgtctgta tgaccagaac agggagcaca accagcagac cgttctgtcc
      481 gataccgaga agcccggcac tgaacgcaag tggccagcag gacagtggtt tttggggcgt
      541 atcttcgatc cctggtgaca ttataatgtt tcaactccat gtattaattg tgtttatttg
      601 taaaattaat ttatctgaca ataacattta ttattgataa tgagaatcat tattgacata
      661 attgttatta ttttgctgtg tgggagctgt ttgactatga ccctgccctc tgaaaaacca
      721 gccacagatg tggctgcgca gtgcttcctg aatgcactga ttcgtgaaac cacagactgg
      781 aaactgacag aatacccgcc agacgaattg ctcatcccgc tggatgagca gaaatcgctc
      841 catttcagag tggcttattt ctccccaacc cagcatcacc gctttgcatt tcctgcacgt
      901 ctggtcacgg catcaggcag ttatcctgtc gactttacca ctctctcccg gcttattatt
      961 gataagctac gccatcaact gtttctgccc gttcccctct gcgaaacttt ccaccagcgc
     1021 gtgctggaaa gccatgtcca tacgcaacag gcaattgatg cccgtcatga ctgggccgct
     1081 ctgcgtgaaa aagcgttgaa ttttggcgag gctgagcagg cgctgctgac aggacacgct
     1141 ttccaccctg cgcctaagtc tcatgaaccg tttaaccggc gggaggctga acgctacctg
     1201 cctgatatgg caccccactt cccactgcgg tggttttcgg tggataaaac gcaaatcgcc
     1261 ggtgaaagtt tgcatctcaa ccttcaacag cggctgacgc gatttgccgc agagaatgcg
     1321 cctcagttac tcaacgaatt aagtgacaac caatggctgt tcccgttgca cccgtggcag
     1381 ggggaatatc ttttgcagca ggggtggtgc caggcacttg ttgctaaagg gctgattaaa
     1441 gacttaggtg aggccggcac gtcgtggctg ccgaccacct cttcccgttc cctctactgt
     1501 gccaccagcc gcgatatgat caagttctcc ctgagcgtac ggctgaccaa ctccatccgt
     1561 actctgtccg tgaaagaagt gaagcgtggg atgcgcctgg cacgcctggc tcaaaccgac
     1621 ggctggcaga tgctacaggt ccgcttcccg actttccggg taatgcagga ggatggctgg
     1681 gccgggctgc gcgatcttaa cggcaacatc atgcaggaaa gtctgtttgc cctgcgtgaa
     1741 aatctgctgg tggatcagcc gaaaagccag actaacgtac tggtctccct gactcaggcc
     1801 gcacctgatg gcggtgattc gctgctggtt tcggcggtaa aacgcctgag cgatcgcctc
     1861 ggtatcactg tgcaacaggc cgcccatgca tgggtcgatg catactgtca gcaagtgcta
     1921 aagccgctgt ttacggctga agcggattac ggcctggtgc tgctggcgca tcagcaaaat
     1981 attcttgtcc agatgcttgg ggatctgccg gtcggattta tttaccgtga ctgtcagggc
     2041 agcgctttta tgcctcacgc gacagactgg ctcgattcta ttggcgaggc gcaggcggaa
     2101 aatattttca cccatgagca gttgctgcgc tatttccctt attacctgct ggttaactcc
     2161 acttttgctg tgaccgctgc gctgggggct gccgggctgg acagcgaatc gaatctgatg
     2221 gctcgtgtac gagcatcgct ggctgaagtg cgtgatcagg tgactcataa aacctgtctc
     2281 aactacgtgc tggaaagtcc gtactggaac gtaaaaggta actttttctg ttatctgaac
     2341 gatcataacg agaacaccat tgttgaccct tcggtgatct atttcgattt cgctaacccg
     2401 ctgcaggctc aggaggtctg aatgtctgag gcaaacatta ttcacagcag atatggactg
     2461 cgctgtgaaa aactcgacaa gcccctgaat cttggctggg gactggacaa tagcgcggtg
     2521 ttgcactgtc ccggggagct gccgacaggg tggctgtgcg atgcgctgga tcagatattt
     2581 atcgccgctc cacaactgtc agcagtggct ctcccctggg ccgaatggcg tgaggagcca
     2641 caggcgctga cgctttttgg gcaggtaaaa agcgacatta tccatcgtac tgccttctgg
     2701 cagttaccgt tatggttgag ttcaccggca aaccgggcct ccggtgaaat ggtttttgat
     2761 gcagagcgtg agatttattt cccgcagcgc ccaccccgtc cacagggtga ggtttaccgc
     2821 cgttacgatc cacgtattcg caggatgctg agtttccgca ttgccgatcc cgtttctgat
     2881 gcagaacgtt tcacccgctg gatgaatgat ccgcgcgttg agtatttctg ggagcaaagt
     2941 ggctcactgg aggtacagac cgcctatctg gagcgccagc tcaccggtaa acatgcattc
     3001 ccgctgattg ggtgcttcga cgatcggcca ttcagctatt tcgaaatcta ctgggcggcg
     3061 gaagaccgca ttggtcgtca ctattcgtgg cagccctttg accgtggcct gcatctgctg
     3121 gttggtgaac agcaatggcg cggggcccac tatgtgcaaa gctggctgcg cgggctgaca
     3181 cattacctgc tgctggatga gccccgtacg cagcgcacag tactggagcc acgcaccgat
     3241 aacctgcgcc tgttccgcca tcttgagcct gcgggatacc ggacaattaa agagttcgac
     3301 ttcccacata agcgctcgcg catggtgatg gcggatcgtc atcacttctt cacggaggtc
     3361 ggtctgtaat gaatcacaag gactgggatt ttgttaaccg tcggctggtg gcaaaaatgt
     3421 tgtctgagat ggagtatgag caggttttcc acgcggaatc tcagggcgat gaccactact
     3481 gcattaacct gccgggagca caatggcgct tcatcgctga acgtggtatc tggggctggc
     3541 tgtggattga tgcccaaacg ttgcgctgca cggacgaacc agtactggcg cagacgctgc
     3601 tgatgcagct aaagccggtg ctgtcaatga gtgatgccac tgttgcagag catatgcagg
     3661 atttgtatgc cacgctactg ggcgacctgc aactgctgaa ggcccgtcgc ggactgagtg
     3721 ccagtgacct gattaatctt gatgccgatc gtctgcaatg cctgctgagc ggtcatccta
     3781 aattcgtttt taataaaggt cgccgtggct ggggcaaaga ggcgctggaa cgatatgcgc
     3841 cagagtatac caacaccttc agactgcact ggctggcggt aaaacgtgaa catatgatct
     3901 ggcgctgcga taacgatctg gatattcagc agttgttgac tgccgcaatg gatccgcagg
     3961 agtttacacg cttcagtcag gtctggcagg aaaacggact ggatcacaac tggctgccgc
     4021 tgccggtaca tccgtggcag tggcagcaaa aaatcgctac cgacttcatc gctgattttg
     4081 ccgaaggcag gatggtgtct ctcggcgagt ttggcgacca gtggctcgcc cagcagtcgc
     4141 tgcgtaccct gaccaacgcc agtcgccggg gcgggctgga tatcaagctg ccgctgacca
     4201 tctacaacac ctcatgctac cgggggatcc ctggcagata catcgctgcc ggaccactgg
     4261 cttcacgctg gctacaacag gtttttgcga ccgacgccac cctggtgcaa agcggcgcag
     4321 tgatacttgg tgaaccggct gcaggctatg tctcccatga aggctatgcc gcgcttgccc
     4381 gggctcccta tcgctaccag gaaatgcttg gtgttatctg gcgggagaat ccgtgccgct
     4441 ggctgaaacc ggatgaaagt ccggttctga tggcaacact gatggagtgc gacgaaaaca
     4501 atcagccgct ggcaggcgca tatatagacc gctccgggct ggacgctgaa acctggctta
     4561 cgcaactgtt ccgggtggtc gtggttcctc tgtatcacct gctttgccgc tacggtgtcg
     4621 cgcttattgc acatggacaa aatataactc tcgccatgaa agagggggtt ccacagcgtg
     4681 ttctgctgaa agacttccag ggcgatatgc gactggtgaa agaagagttc cccgaaatgg
     4741 actctttgcc tcaggaggtt cgtgatgtta catcccgcct gagtgcggac tacttaatcc
     4801 atgatttgca gacgggtcac ttcgtgacag tactgcgttt tatttcgcca ctgatggttc
     4861 gtcttggcgt acctgaaagg cgattttatc aactgctggc agcagtgttg agtgattaca
     4921 tgaaaaaaca tccacaaatg tcagagcgtt ttgcgctttt ctcacttttc aggccacaaa
     4981 tcattcgcgt ggtgctgaac ccggtaaaac tgacctggcc tgatctggat ggcggcagcc
     5041 gcatgctgcc gaattacctt gaggatctgc aaaatccgct gtggctggta actcaggaat
     5101 atgaatcatg aaaaaaagtg tcgattttat tggtgtaggg acagggccat ttaatctcag
     5161 cattgctgcg ttgtcacatc agatcgaaga actggactgt ctcttctttg atgaacatcc
     5221 tcatttttcc tggcatccgg gtatgctggt accggattgt catatgcaga ccgtctttct
     5281 gaaagatctg gtcagtgctg ttgcacctac aaatccctac agttttgtta actatctggt
     5341 gaagcacaaa aagttctatc gcttccttac aagcagacta cgtacagtat cccgtgaaga
     5401 gttttctgac tacctccgct gggctgctga agatatgaat aacctgtatt tcagtcatac
     5461 cgttgaaaac attgatttcg ataaaaaacg tcgattgttt ctggtgcaaa ccagccaggg
     5521 agaatatttt gcccgcaata tctgccttgg tacaggaaaa caaccttatt taccaccctg
     5581 tgtgaagcat atgacacaat cctgtttcca tgccagtgaa atgaatcttc gtcggccgga
     5641 ccttagtgga aaacggataa ccgtggttgg tggaggacag agtggtgcag acctgttcct
     5701 taatgcatta cgcggggaat ggggagaagc ggcggaaata aactgggtct cccggcgtaa
     5761 taattttaac gcactggatg aggctgcttt tgctgatgag tattttacac ctgaatatat
     5821 ttcaggcttc tccggactgg aggaagatat tcgccatcag ttactggatg agcagaaaat
     5881 gacatcggat ggcatcactg ccgattcttt actgaccatt tatcgtgagt tgtaccaccg
     5941 ttttgaagtt ctgagaaaac caagaaatat ccgtctgcta cccagccgct cggtaacaac
     6001 tctggaaagt agtggtcctg gctggaagtt gctgatggag catcatctgg atcagggcag
     6061 ggagagcctg gaaagtgatg tggtgatttt cgccacaggt taccgttctg cgttgccaca
     6121 aatacttccc tcactgatgc ccctgatcac catgcacgat aagaacacct ttaaagtgcg
     6181 tgatgacttc actctggaat ggagtggccc gaaagagaac aacatcttcg tggtcaacgc
     6241 cagtatgcaa acccatggca tcgccgaacc ccagctcagc ctgatggcat ggagatctgc
     6301 acgtattctt aatcgcgtaa tgggacgtga tttattcgat ctcagtatgc cgcccgccct
     6361 gattcagtgg cgcagcggca cctagggaaa acgcagccgg aggctgcttc tttaactcgc
     6421 tacacagcat ctttgggctg attttttccg cccgtatgga ggaataatga tgataagcaa
     6481 aaagtatacg ctttgggctc tcaacccact gcttcttacc atgatggcgc cagcagtcgc
     6541 tcaacaaacc gatgatgaaa cgttcgtggt gtctgccaac cgcagcaatc gcaccgtagc
     6601 ggagatggcg caaaccacct gggttatcga aaacgccgaa ctggaacagc agattcaggg
     6661 cggcaaagag cttaaagacg cactggctca gctgatccct ggccttgacg tcagcagccg
     6721 gagccgcacc aactacggta tgaatgtgcg tggccgcccg ctggtcgtgc tggttgacgg
     6781 cgtgcgtctc aactcttcac gtaccgacag ccgacaactg gactctatag atccttttaa
     6841 tatcgaccat attgaagtga tctccggtgc gacgtccctg tacggcggcg gcagtaccgg
     6901 tggcctgatc aacatcgtga ccaaaaaagg ccagccggaa accatgatgg agtttgaggc
     6961 tggcaccaaa agtggcttta gcagcagtaa agatcacgat gaacgcattg ccggagctgt
     7021 ctccggcgga aatgagcata tctccggacg tctttccgtg gcatatcaga aatttggcgg
     7081 ctggtttgac ggtaacggcg atgccacctt gcttgataac acccagaccg gcctgcagta
     7141 ctccgatcgg ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct
     7201 tcagttgatc acacagtact ataaaagcca gggcgacgac gattacgggc ttaatctcgg
     7261 gaaaggcttc tctgccatca gagggaccag cacgccattc gtcagtaacg ggctgaattc
     7321 cgaccgtatt cccggcactg agcggcattt gatcagcctg cagtactctg acagcgcttt
     7381 tctgggacag gagctggtcg gtcaggttta ctaccgcgat gagtcgttgc gattctaccc
     7441 gttcccgacg gtaaatgcga acaaacaggt gacggctttc tcttcgtcac agcaggacac
     7501 cgaccagtac ggcatgaaac tgactctgaa cagcaaaccg atggacggct ggcaaatcac
     7561 ctgggggctg gatgctgatc atgagcgctt tacctccaac cagatgttct tcgacctggc
     7621 tcaggcaagc gcttccggag ggctgaacaa caagaagatt tacaccaccg ggcgctatcc
     7681 gtcgtatgac atcaccaacc tggcggcctt cctgcaatca gactatgaca tcaataatct
     7741 ctttaccctc aacggtggcg tacgctatca gtacactgaa aacaagattg atgatttcat
     7801 cggctacgcg cagcaacggc agattgccgc cgggaaggct acatccgccg acgccattcc
     7861 tggcggctca gtcgattacg acaacttcct gttcaacgcc ggtctgctga tgcacatcac
     7921 cgaacgccag caggcatggc tcaacttctc ccagggcgtg gagctgccgg acccgggtaa
     7981 atactatggt cgcggcatct atggtgctgc agtgaacggc catcttcctc taacaaagag
     8041 tgtgaacgtc agcgacagca agctggaagg cgtgaaagtc gattcttatg agctgggctg
     8101 gcgctttact ggcaataatc tgcgcaccca aatcgcggcc tactattcga tttctgataa
     8161 gagcgtggtg gcgaataaag atctgaccat cagcgtggtg gacgacaaac gccgtattta
     8221 cggcgtggaa ggtgcggtgg actacctgat tcctgatact gactggagta ccggagtgaa
     8281 cttcaacgtg ctgaaaactg agtcgaaagt gaacggtacc tggcagaaat acgatgtgaa
     8341 gacagcaagc ccatcaaaag cgacagccta cattggctgg gcaccggacc cgtggagtct
     8401 gcgcgtgcag agcaccacct cctttgacgt gagcgacgcg cagggctaca aggtcgatgg
     8461 ctataccacc gtggatctgc tcggcagtta tcagcttccg gtgggtacac tcagcttcag
     8521 cattgaaaac ctcttcgacc gtgactacac cactgtctgg gggcagcgtg caccactgta
     8581 ctacagcccg ggttacggcc cagcgtcact gtacgactac aaaggcaggg gccgaacctt
     8641 tggtctgaac tactctgtgc tgttctgacg gggatgcttt tgcaacaagg gtatgctgta
     8701 aacaacatgg tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa
     8761 gatattccgt ctccggaatg gactgttcac cactgaataa cattatgtct gttttatgga
     8821 tctttgactg aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac
     8881 aagtaagcgt cgtctcagca ccgtctggca gatcctgaaa ttcctgggag gatgatctgc
     8941 gcccaatatc ctgagccgga actttgctgc aatgaaggcc aacgaaaaat gggggagtat
     9001 gttatagagt acttgctaca gggtaaaaag aggtacctgt cgctggttct ccgttcagtt
     9061 taaccgggag attttctcct gcgcctgtca taaagtccgg taatggagat tgttaatacg
     9121 atggtgcacg atgcattgtc agtgcccggc ccggaggatg ccccgtagct acatacggat
     9181 cagggttggc aatatcgaat ggcaggctat aggcaaagtt aaagccccat ggagtagcac
     9241 aaaatatgcc gcacaaagga aacggtctga ataacgcagc gatggagaac ttcttcggca
     9301 cactgcaaaa tcgcatcgtg gtcgatcgct aattcgtcgt gaatcactga acgatgttcc
     9361 atgcgaacag atattcgatc atatcgaaat attaaacatt acccctcccc acattgggca
     9421 aaaaaaaaca aaaaacgatc aattaacgat caattagtat tgattcacat aatctctcat
     9481 cattaatgag aacattgtca catatttaca atatagaact gttactttta gcaagctgtt
     9541 agtaaccaac cgttactaat aattttaatt cttaatactt tgttcattca ataaatgagt
     9601 tgagagaata tatgaataaa atatactccc ttaaatatag tgctgccact ggcggactca
     9661 ttgctgtttc tgaattagcg aaaagagttt ctggtaaaac aaaccgaaaa cttgtagcaa
     9721 caatgttgtc tctggctgtt gccggtacag taaatgcagc aaatattgat atatcaaatg
     9781 tatgggcgag agactatctt gatcttgcac aaaataaagg tattttccag cccggagcaa
     9841 cagacgtaac aatcacttta aaaaacggag ataaattctc tttccataat ctctcaattc
     9901 cggatttttc tggtgcagca gcgagtggcg cagctaccgc aataggaggt tcttatagtg
     9961 ttactgttgc acataacaaa aagaaccctc aggccgcaga aactcaggtt tacgctcagt
    10021 cttcttacaa ggttgttgac agaagaaatt ccaatgattt tgagattcag aggttaaata
    10081 aatttgttgt ggaaacagta ggtgccaccc cggcagagac caaccctaca acatattctg
    10141 atgcattaga acgctacggt atagtcactt ctgacggttc aaaaaaaatc ataggttttc
    10201 gtgctggctc tggaggaaca tcatttatta atggtgaatc caaaatctca acaaattcag
    10261 catatagcca tgatctgtta agtgctagtc tatttgaggt cacccaatgg gactcatacg
    10321 gcatgatgat ttataaaaat gataaaacat ttcgtaatct tgaaatattc ggagacagcg
    10381 gctctggagc atacttatat gataacaaac tagaaaaatg ggtattagtc ggaacaaccc
    10441 atggtattgc cagcgttaat ggtgaccaac tgacatggat aacaaaatac aatgataaac
    10501 tggttagtga gttaaaagat acctatagtc ataaaataaa tctgaatggc aataatgtaa
    10561 ccataaaaaa cacagatata acattacacc aaaacaatgc agataccact ggtactcaag
    10621 aaaaaataac taaagacaaa gatattgtgt tcacaaatgg gggaaatgtc ctgtttaagg
    10681 ataatttgga ttttggtagc ggtggtatta tctttgacga aggccatgaa tataacataa
    10741 acggtcaggg atttacattt aaaggagcag gaattgatat cggaaaagaa agcattgtaa
    10801 actggaatgc attgtattcc agtgatgatg ttttacacaa aataggcccc ggtactctga
    10861 atgttcaaaa aaaacagggg gcaaatataa agataggtga aggaaatgtt attcttaatg
    10921 aagaaggaac atttaacaat atataccttg caagcggaaa tggtaaggta atactaaata
    10981 aagataattc ccttggcaat gatcaatatg cggggatatt ttttactaaa cgtggtggca
    11041 cgctagattt aaatggacac aatcagactt ttactagaat tgccgccact gacgatggaa
    11101 caacaataac taactcagat acaacgaaag aagccgttct ggcaatcaat aacgaagact
    11161 cctacatata tcatgggaac ataaatggca atataaaact aacgcacaat attaattctc
    11221 aggataagaa aactaatgca aaattaattc tggatggtag tgtcaacaca aaaaatgatg
    11281 ttgaagtcag taatgccagt cttaccatgc aaggccatgc aacagagcat gcaatattca
    11341 gaagcacagc gaatcattgc tccctggtat ttctttgtgg aacggactgg gtcaccgttt
    11401 tgaaagaaac agagagttca tataataaaa agttcaattc tgatcacaaa agtaataatc
    11461 agcagacctc atttgatcag ccagactgga aaaccggggt gtttaaattt gatacattac
    11521 acctgaacaa tgctgacttt tcaatatcac gcaatgccaa tgttgaagga aatatatcag
    11581 caaataaatc agctatcaca atcggcgata aaaatgctta cattgataat cttgcaggga
    11641 aaaatattac taataatggt tttgacttca aacaaactat cagtactaat ctatccatag
    11701 gagaaactaa atttacaggt ggcatcactg cacataacag ccaaatagcc ataggtgatc
    11761 aagctgtagt tacacttaat ggtgcaacct ttctgaataa tactcctata agtatagata
    11821 aaggagcaaa agttatagca caaaattcca tgttcacaac aaaaggtatt gatatctccg
    11881 gtgagctgac tatgatggga atccctgaac agaatagtaa aactgtaacg ccgggtctcc
    11941 actacgctgc tgatggattc aggctgagtg gtggaaatgc aaatttcatt gccagaaata
    12001 tggcatctgt caccggaaat atttatgctg atgatgcagc aaccattact ctgggacagc
    12061 ctgaaactga aacaccgact atatcgtctg cttatcaggc atgggcagag actcttttgt
    12121 atggctttga taccgcctat cgaggcgcaa taacagcccc caaagctaca gttagcatga
    12181 ataatgcgat ctggcatcta aatagccagt catcaattaa tcgtctagaa acaaaagaca
    12241 gtatggtgcg ttttactggt gataatggga agtttacaac ccttacagtg gacaacctta
    12301 ctatagatga cagtgcattt gtgctgcgtg caaatctggc ccaagcagat cagcttgttg
    12361 tcaataaatc gttgtctggt aaaaacaacc ttctgttagt cgacttcatt gagaaaaatg
    12421 gaaacagcaa cggactgaat atcgatctgg tcagcgcacc aaaaggaact gcagtagatg
    12481 tctttaaagc tacgactcgg agtattggct tcagtgatgt aacaccggtt atcgagcaaa
    12541 agaacgatac agacaaagca acatggactc tgatcggcta taaatctgtg gccaacgccg
    12601 atgcggctaa aaaggcaaca ttactgatgt caggcggcta taaagccttc cttgctgagg
    12661 tcaacaacct taacaaacgt atgggtgatc tgcgtgacat taacggtgag tccggtgcat
    12721 gggcccgaat catgagcgga accgggtctg ccggcggtgg attcagtgac aactacaccc
    12781 acgttcaggt cggtgcggat aacaaacatg aactcgatgg ccttgacctc ttcaccgggg
    12841 tgaccatgac ctataccgac agccatgcag gcagtgatgc cttcagtggt gaaacgaagt
    12901 ctgtgggtgc cggtctctat gcctctgcca tgtttgagtc cggagcatat atcgacctca
    12961 tcggtaagta cgttcaccat gacaacgagt ataccgcaac tttcgccggc cttggcacca
    13021 gagactacag ctcccactcc tggtatgccg gtgcggaagt cggttaccgt taccatgtaa
    13081 ctgactctgc atggattgag ccgcaggcgg aacttgttta cggtgctgta tccgggaaac
    13141 agttctcctg gaaggaccag ggaatgaacc tcaccatgaa ggataaggac tttaatccgc
    13201 tgattgggcg taccggtgtt gatgtgggta aatccttctc gggtaaggac tggaaagtca
    13261 cagcccgcgc cggccttggc taccagtttg acctgtttgc caacggtgaa accgtactgc
    13321 gtgatgcgtc cggtgagaaa cgtatcaaag gtgaaaaaga cggtcgtatg ctcatgaatg
    13381 ttggtctcaa cgctgaaatt cgcgataatc ttcgcttcgg tcttgagttt gagaaatcgg
    13441 catttggtaa atacaacgtg gataacgcga tcaacgccaa cttccgttac tctttctgat
    13501 aacagcccgg gccgcgtttg cggcccttct tctaccggag agaatatgta ttaccctgtg
    13561 acagactata tcgctcttgc tctcattatt agctttcttt ttctgacatt atttatctgc
    13621 ctgttatgcc ttaaacatga gcgaataaaa aaagaaacta tcaggcaaaa aaatgcacat
    13681 attctggatc atggctggaa tgcaactgag gtagcctgag tttaacggac actccttcct
    13741 gaaatagaat ggcatcagaa ggagctaata atgagcagaa aaacccaacg ttactctaaa
    13801 gagttcaaag ccgaagctgt cagaacggtt cttgaaaatc aactttcgat cagtgaaggc
    13861 gcttcccgat tatctcttcc tgaaggcact ttaggacaat gggttaccgc cgccagaaaa
    13921 gggctcggta ctcctggttc ccgcacggtg gctgaactgg aatctgaaat tctgcaactg
    13981 cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc aactgtagat
    14041 tcaattggtc aacgcaacag ttatgtgaaa acatggggtt gcggaggttt tttgaatgag
    14101 acgaacattt acagcagagg aaaaagcctc tgtttttgaa ctatggaaga acggaacagg
    14161 cttcagtgaa atagcgaata tcctgggttc aaaacccgga acgatcttca ctatgttaag
    14221 ggatactggc ggcataaaac cccatgagcg taagcgggct gtagctcacc tgacactgtc
    14281 tgagcgcgag gagatacgag ctggtttgtc agccaaaatg agcattcgtg cgatagctac
    14341 tgcgctgaat cgcagtcctt cgacgatctc acgtgaagtt cagcgtaatc ggggcagacg
    14401 ctattacaaa gctgttgatg ctaataaccg agccaacaga atggcgaaaa ggccaaaacc
    14461 gtgcttactg gatcaaaatt taccattgcg aaagcttgtt ctggaaaagc tggagatgaa
    14521 atggtctcca gagcaaatat caggatggtt aaggcgaaca aaaccacgtc aaaaaacgct
    14581 gcgaatatca cctgagacaa tttataaaac gctgtacttt cgtagccgtg aagcgctaca
    14641 ccacctgaat atacagcatc tgcgacggtc gcatagcctt cgccatggca ggcgtcatac
    14701 ccgcaaaggc gaaagaggta cgattaacat agtgaacgga acaccaattc acgaacgttc
    14761 ccgaaatatc gataacagac gctctctggg acattgggag ggcgatttag tctcaggtac
    14821 aaaaaactct catatagcca cacttgtaga ccgaaaatca cgttatacga tcatccttag
    14881 actcaggggc aaagattctg tctcagtaaa tcaggctctt accgacaaat tcctgagttt
    14941 accgtcagaa ctcagaaaat cactgacatg ggacagagga atggaactgg ccagacatct
    15001 agaatttact gtcagcaccg gcgttaaagt ttacttctgc gatcctcaga gtccttggca
    15061 gcggggaaca aatgagaaca caaatgggct aattcggcag tactttccta aaaagacatg
    15121 tcttgcccaa tatactcaac atgaactaga tctggttgct gctcagctaa acaacagacc
    15181 gagaaagaca ctgaagttca aaacaccgaa agagataatt gaaaggggtg ttgcattgac
    15241 agattgaatc tacaaccgcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga
    15301 acaatggcga caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag
    15361 cggttattac aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct
    15421 aaaactggag attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct
    15481 ccagacggag ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg
    15541 taaggagcta aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa
    15601 ccacaatctg ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa
    15661 tcaggtctgg gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc
    15721 tggcatcaaa gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac
    15781 aaaagagctg acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg
    15841 gctaatccac cactctgatc gaggttcaca gtactgcgca tacgattacc gggtcataca
    15901 ggagcagttt ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc
    15961 gatggaaagc ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa
    16021 ccgggatgaa gccatctcag taatacggga atacattgag attttctaca atcgtcagcg
    16081 tcgtcactct cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat
    16141 ggctgcttaa aaaaagaaca aatggttgtg tccgctattg ccagtacacc tcaaactgag
    16201 ttctcatggt tccgatacgg acagtataac gaaacgggta tttacatctc gatcgagaag
    16261 accattatca ttacaatcac cgtttctggt gggtgcttta aaaaagaata cagcattgtc
    16321 tcccacatgc tggtcactga caccataaca gaggccaccc tgtacgaaaa cgggctgtat
    16381 acgcgccaca tcagactcag tcgcccagta tctgacagca aatacccctt gccacccgga
    16441 tctcagctca ttaaaaatat gaccttacga ctgcgcctgc aggatcagca ggaagaaaca
    16501 tccgtgacat tatttcaggg aaaaataagt accgacggaa ataactattc catcatcaaa
    16561 ggtaaagtaa gctcagtact tctgttatta aaaatgctgc agattaatca tgcctgatct
    16621 ctgtcccggt attttagccc gaggtatatt ctgaaatatc attcatcatg accggcgttt
    16681 atacaaacgc cagtcattcg ctaacagctt caccgttcct tcccccatcc aggttccgca
    16741 tgagcgtgtt gtaatgttca taagcgtatt atcaggattg tctggaaaat tcccgatact
    16801 ttggactatt actcctgaac aactgtaagc gtacagcctg aaccgtctgg tcagaatctg
    16861 acgaattaga caaagtggtg tccaccaaat aagtagtggg aaccaaagtg tcagatatgc
    16921 agaaaaatgt gactcccggc aggcgaaaag gctgccctaa ttatcctccc gaatttaaac
    16981 agcagctcgt tgggggatgc ggacgatttc ctggactctc aggagataaa acatgtttac
    17041 tgaagaagaa aaaatccgcg ccattgagct ttatttcaaa tatggtaaga agctggctcc
    17101 cgtcgtacgc gaactgggct acccttcgaa aagaaatctg cggcgttgga tccgctcatg
    17161 ggaagcaggt ggtggcgcaa aagaatccat tcgtcataag caccgttact ccgatgagca
    17221 aaaacaggtt gccgttgaac attatcttaa ccacggctgc tgcctggcgt ttaccagcag
    17281 agccctgggt tatccctgca ctgatgtact cgctcgctgg gttaatgaac tttatcccga
    17341 cagacgacgt atatttacca gtaaggccaa cccggttgcg ccctttgaac cagaagtcaa
    17401 acgtcaggct gtcatggcac tctgtacacg acaagtatca gccagtgaga tcgccaggcg
    17461 tattggtgtc agtcgtgcgg tattgtataa atagaaagat gaaattatcg gcaacagtgc
    17521 ttaccagact atgcgtaaac ataacgaacc ttccctggag gcagaacgcg atgcgttgcg
    17581 ggaggaagtc gcccgactga atcaggaaat acgccgccgg cagatggagc tggatattct
    17641 gaaaaaggcg gaggaaatca taaaaaaaga cccgggcatc agtatcagtc acctgaacaa
    17701 cagagaaaaa acaaagatcg ctgatgccct gaggcaaaca tatcccctga cagaattact
    17761 tcatgttctg ggccttaccc gtagcagtta tttttatcac cgggctgcac tgaaagccgg
    17821 tgataaatac gccacgatac gtacgatgct gacagatata tttaacagta attaccagtg
    17881 ttatggctat cgtcgcctgc atgcgatgct caggcatgag ggggggcggc tatcagaaaa
    17941 ggttgtacgc agacttatgg tggaggaaca gcttgtcgtc agccgtaacc gtcgtcgtcg
    18001 ctacagctca tattgcggag aaatcggacc ggctccggat aaccttatcg ccagagattt
    18061 taaggcgtag caacctaatc agaaatggct gacagatatc acggagttcc aactccctgc
    18121 aggtaaagtc tggctgtcac cggtggtgga ctgcttcgat ggaaaagtta ttagctggtc
    18181 tctcagtaca cgccccgatg ctgagctggt caacactatg ctggatagcg ctgtcgaaac
    18241 gttaaatgct ggcgaacgac cggtgataca cagtgacaga ggtgggcatt atcgctggcc
    18301 aggctggctg gaaagagtga atgcagcagg tcttattcgc tcaatgtccc gtaaaggatg
    18361 ttcacctgat aatgccgcat gcgaaggttt tttcggcaga ctgaaaacgg aaatgtatta
    18421 tgggcgtaaa tggtcgggca tcacgccaga aaagctcatg cagcatgtag atgcttacat
    18481 cagatggtat aacgagcggc gtataaaatt atcgctgggt gcagtcagcc ctgaaatgta
    18541 ccgccaacaa tgtgggctgg cataataaag cagtctagga aatcgtccgc atccccaatt
    18601 caggggcggt tttttcttta cggtttttcg ccataaagac tattactatc agactgacaa
    18661 gactacccgc gaaagccggg agttccgcgc caagattaac agccaccaga tactggggaa
    18721 ttaatgtact gacaccacac atcagggtaa ttagtccaac gcctcgtata gtcttcatac
    18781 caccaccgat aatcgcgacg atgacgaaag gaagcagaat gttgaacagt gccagttgaa
    18841 gaataattgt cccgctgagt gtggttacag gtaaattaac ttgttcagca agtacagaga
    18901 caggtatacc tactgcccca aacgctgtag ggactgtatt agcaaccagt gatgcaatcg
    18961 ctgcttttag tgggttaaag cccagagcaa tcagtatacc aatggggatt gctaccgctg
    19021 taccgtatcc ggctgccgcc tcaagaaatc caccaaaaca ccatgagata agaagtacct
    19081 ggatacgttt gtcatcacta acactcgctc aatcatttgg tctgctatct caacagattg
    19141 ctttcaacat tattgtcaga atctggagca gattgaagat cgcatacttc aactcgatca
    19201 ggaaatatct cgcctgttac ctgagtgatc tctgtgcaat ctggtctgcc agttgcaggc
    19261 tcttaaaggt gttggtcaac taatcgcaat aaccctggtt gctgaactgg gtgatttttc
    19321 gcgattttcc aatcctgaac agctcatggc ttttctggga ctggtgccag gtgaatattc
    19381 cagcggaaac agcattcgcc ccagaggaat aacaaaagtt ggaaacagcg aactgagaca
    19441 tctgctttac ggatatcaaa taaattcaga ttatttaaca gtaaaacccc acttatgtga
    19501 tgaatctcat aaaaaagcaa agtgaagtaa taatatccaa tttatccctc attccttaag
    19561 taagcgtaca ctggccgcgt acaccatggc ataatgatat gttgttttac tgatatttcc
    19621 ggctggtaaa atccccgacg gcccggctga agctggtgca ggaaagcggg ttaattgtca
    19681 ctacgcggga taacctgtct ggctggatac aacgtattgc cacagatatg cagcaactgc
    19741 gtcagtccgg aatgtcagtc atctccgggg atgttggggt ctcaggcaca ctgaccggcc
    19801 ccccgtgttg tttccgggta ctgtttttga tttatgcaac aaagtattgc agcgtcgaat
    19861 atgagctatg gccagatttt tgttaatcgt ggttgttgca acatgtaagg cgaatgacca
    19921 cactattctg gcacgctcgt aatcacgctg aaattccgct tcctgtgctg ccgtgaagag
    19981 ttccgaccag gacatacggc gaagtgtatg tctgtcagta atatccgtca tgagctctca
    20041 gcttcctcct tcgtatttcc gtcagaaaca caaacgcccc gcagagaaaa ttcgccaaca
    20101 ttctcctttt taatggcgtg caatgcatcg cgacaggccc gttcggtatg aaatgcctgg
    20161 ctgtgcaccg ccagtgtggg ctgccgggca tcaccggaag agacattaaa gggagatgaa
    20221 agccagaaaa tcagtagcca catgcgtcac acctcgtgcg ggtatctggc cagaatattg
    20281 agcgcctgta atggagcttc atgcatttca cggatacaac gggcatcggt gatgttttcc
    20341 ctgtaatgcg tggtttccag catcatatga tgcatatgca gcatccgggc cagggctgca
    20401 cggagttctt caatatgttg ttcatgggaa tgctgacggg gtggtatccg gcagagaggg
    20461 ataacctcca catcatccgg gtgcgcgcga agccacgcgt cagtgactgt aatgtcgtca
    20521 gtgtaaaacc atgtgccgga tgtcccgttg gccagaaaac gcattttcca ggcttctggg
    20581 gtggcggttg tgctgttatc tttcatggtg ccaccactca ttcaacaggc ttaatgctgt
    20641 ccaggcgcag tctgctcaga ctgtcgtggc tgccacagcg gcgtcctgta cttttgctgt
    20701 agcactcctg ccccctgata caccatgccg ttggcgtttc gtagaggctg acacgtcggg
    20761 ttttatcttt actggctaga agcccggtgt gagtttttgt ctttttcatc ggtggggtac
    20821 aaacgcagaa attttgttgt catcagtttt tgcctgcaga ggcctgaaaa gacagcgttt
    20881 tcagaccctg ttcaggttag tgaatattct tacctggcct ttcggcaata tcaatcccct
    20941 gaagttgcat ggatatccca tgcccattga gcatctggct gtccatggaa tggataccgc
    21001 atgtcaccat caggttgtaa caaagcgtac tcagcatggc gagcgcctct tctgaggatt
    21061 tacaatgtgt gttcataagg gccagaaact gctcaacaac atccgggttg atgctggtac
    21121 tgtgtatttc atccttcatt atgtgtcctt gttttttatt attttgcatt tcagaaaata
    21181 acacgatgca tgtaacccag aaaaccgata tgtgcacgcg tagtcacttt ctggcagatt
    21241 ccggtctctg aaacaaaata cgaggtggca tgaagcggat tgtggcggac agggaaagtg
    21301 atttgattcc ggggaaggcg ctgtgcagca gtcttatccg gcagtttaaa ggggtggacg
    21361 gagaccaatg aggcactgga gcagattcag tgcagaggtg acatgattta tcaaacttaa
    21421 ttgtgacaaa atggcgattg agaaggtgca attgacttat ccggattcgg tcgtggcgag
    21481 gtggtgaccc tgcattctgc taaccgggtt atgagccagg agtcagcgat agacaaaggg
    21541 acttatctga ccgggccccc ggaaaaatgg cgggagagaa tgataccttc ctgctgacga
    21601 gaccggactt tgagatgacg agcgatagtg cggcagtgat gtacagcctg atcggtagct
    21661 gcaaaactta acggaatcga gccggaaacg tggctacgcc acgtgatcag tgtaatcaac
    21721 acctggcctg ccaaccgcat gaaagagttg ttgccctgga acgtcactct ctctgtacac
    21781 taatttttac cccacgtcct tcacggtgcg cttacactct ggacaagtat gtaaatgata
    21841 agttaaaaat aatccgtgca tttcctgtga cgatattagt tgttggtaac gaaatctcat
    21901 tagcggatcg gaaatgcact taacctaata cgttgccact gttccgccag gagttacagt
    21961 ggcaacgtat tctaccgtca gtgatagcgt tttgttatta tcagaactga tagttcagcg
    22021 acatccagta atttcgctca ggtatcacat atcctgttgt tgatgatccc gtctggaagt
    22081 aatcaccggc atacagcgta ctcttaccgg cactgtacag gctcacgtca ctgtaatcct
    22141 tgttgagcag gttattcacc gcagcattca gcgtcagggc atccgtcatc ttccacgaca
    22201 gacctgcatc caccaccgtc caggctttca ggtattctcc tttctcatca tacactttct
    22261 tctgtacagc gctcagtgac gaataattct gggtgaaacg tggtgttttc ccgcgataac
    22321 gggcacccag ccatgatgcc acctcttcgg tgatctgcca gttcagtttc gcattcacca
    22381 tgtgttcagg ggtataactc agcggcgcac ctttgttatc accatcacgt tgttcacttc
    22441 gggtccaggt gtaattcagt gacagcgtga catcctctga ccacagcggc aatgtgccgg
    22501 caaattccac accatgcaac cgggcctttc cgctgtttac atagctattg gtgttatcat
    22561 ttatggaata agagacaatc ttgttggaga agtcagtcat aaaacctgtg acattggcat
    22621 tcagaccggc ggggttatcg taatacaccc cagcctcata actgacgctc tcttccggct
    22681 tcaggtcggg gttaccaagt agatttgttt ttccctgccc ggatacacca ctaatccctt
    22741 tatgtagctg ccccattctg ggtgccttat atcccgtggt cacaccgcct ttcagcgtcc
    22801 aggcatctgc cacatcccag accagatatg cacgcggact gaagtgtccc ccgaattgct
    22861 catgatgttc atagcggctg cccacagtca gcgcaagtgc atccgtgaga tgccactcat
    22921 cctcagcaaa taccgaccag cttttctgcc ggaaagtttc acctgtgctg gcaaggacaa
    22981 ctccgtcttt catggacgag ctctgaaact cgccccccac cgtaaccaga tgagattctc
    23041 ccagaggggt aagcagtaat gaattcagga taaggttcga ttccttaagc tcccgcggct
    23101 gaccggcaag cccccatttg tcgcgcttca gtacactgcg tacgagctca cgacctttat
    23161 tttctgtctc gttccagttc agatacgatt tccatgttcc gaaggtgaaa gtatgatcat
    23221 agccagctga aattttgttt cgctcatagc gcagggtccg gtcatatccc cccgtcagac
    23281 tccccagttg cccatcccgg ttatcataac gctgccgggt ggtatccata tcaaaccaga
    23341 gcacatcctg cgccgacgcc ttccagtcaa gacgtgcacc aagattataa ttctgtgact
    23401 ccgtgggata aggaatacgc gtggctgctg tatcgctcag tgatgtgacc gatgagccct
    23461 gacgctgttg tgtgctaccg cgtacctgca ggctgacaga atcatccaca aggggaccac
    23521 tgctccagaa attaaactgg ctgctgttac cccatttgtt gctttcctgc agattcagcc
    23581 ctgcattgac ggaagagagc catttgtctg cattctttct ggtaatgata ttcaccacac
    23641 cgcccatcgc atcagagcca tacagtgtgg acatcggccc cctgataacc tcaatacgct
    23701 caatggcggc cagagggggc atgaacccgg tattcatggc agaaaaaccg ttgggagtca
    23761 cgtcactgct tccgccctga cgaacaccat caatcagtat cagcgtgtaa ctggctggca
    23821 ttcctcggat gctgatttcc agcccaccgg ttttacccgt accactttca acatccacac
    23881 cctctactga tctcagagcc tccgccagat cgtggtactg gctggactgc aattcctcct
    23941 ggctaatcac agaaacactg gcggctgcgt tagtcagctt tttctcatag ccggatgcag
    24001 agacaatcat cacatcctct gcagcagcta tgctgctggc tgaaaatccg agacagggaa
    24061 tgactacgga agccagagtg gttattcgca tattactaat ctccgatcat gttaaccggc
    24121 aagacaatac ataaatacaa acaataatga gaataattta tatttgcctt ttacaaaaaa
    24181 taaagattat tgacaaaact aaatataaat aacgactgcc tcggcatgcc agttaacagc
    24241 tatccgtatg gcgtgttcgg ttttgcgaat aaaaaatggt tactgtagaa gcggatttcc
    24301 accacatgtc aaccatgaat ggtcagtggt gcgagacgac tgggatgatt tggtcgatga
    24361 cttcttccgg cgaatactga tagaaacatt gaaatattgt aagagaaagc gttcaaaatt
    24421 ctgtggcacg tgaaaaattc tacagcgtca tcaccttatt cagtcgacct tcaaaataaa
    24481 cgtttaacag agacaatcac catttggatt gtccgctgag gaggcgaacg atttcctaga
    24541 cagctttatc atgtcagccc acattgttgg cgctgacaat ggatttgccc ctatatttcc
    24601 agacacctgt tatcacttaa cccatcactg gcccactgcc gcagatattc ccgtggcgag
    24661 cgataaccca gcgcactatg cggatgccat tcgttataat gctcgaacgc ctctgcaagg
    24721 ttctttgctg ccgttaaccc gtctggtttg ggcatgatac ttatgtagtc acgctttatc
    24781 gttttcacga agctctctgc tattccgtta ctctccggac tccgcactgc cgtgttcttc
    24841 ggttcaagtc ccaacatcct ggcgaactga cgtgtttcat tcgcccggta gcatgaacca
    24901 ttatccgtca gccactccac tggagacgcc ggaagctcgc tgccaaagcg gcgttccact
    24961 gctcccagca tgacgtcctg tactgtttca ctgtcgaagc cacccgttgt gaccgcccag
    25021 tgcagtgcct cacggtcaca gcagtccagc gcgaacgtga cccgcagttt ttctccgtta
    25081 tcacagcgga actcaaaccc gtcagagcac catcgctgat tactttcttt cacagccact
    25141 ctgccggtat gtgcccgttt cgatggcggt acagcgggtt ttcgctcaag caacagcgca
    25201 ttctggcgca tggtccggta aacacgtttg gcattgatcg caggcatacc atcaagttct
    25261 gtttgtctgc gaagcagcgc ccatacccga cgataaccat atgtgggtag ctctccgata
    25321 acatggtgta tacggcgaag cacatccgta tcatccgtgt gacggctgcg gcggccgtcc
    25381 ttccagtcat cggctcgtct gagaatgacg tgcaactgcg cacgcgacac ccggagacaa
    25441 cggctgacga agcttactcc ccatccccgg gcaataaggg cgcgtgcgct atccactttt
    25501 ttgctcgccc atattcaacg gcttctttaa ggagttcatt ttccatcgtt tttttgccga
    25561 gcagacgctg gagttcttta atctgcttca tggcggcagc aagttcagag gcaggaatga
    25621 cctgttctcc ggcagccaca gcagtaagac ttccctcctg gtattgctta cgccagagaa
    25681 atagctggct ggctgccaca ccatgttgcc gggcaacaag ggagaccgtc attcccggtt
    25741 caaagctctg ctgaacgata gcgatctttt cctgtgtagt acgccgtctg cgtttctccg
    25801 gccctaagac atcaatcatc tgttctccaa tgactagtct aaaaactagt attaagacta
    25861 tcacttattt aagtgatatt ggttgtctgg agattcaggg ggccagtcta accactccgt
    25921 catgtctctc agagaatcat gtcagagctg tagatatatc ctgaacttgt ctacaaataa
    25981 tttcttattt tttgcgcata acattcagca ataactcccc ttctggccat tgaattaaaa
    26041 tgtgtacttc tctgtgacgt tgtccagctt aattctgtcc tccatgatga ccccagatac
    26101 ccgacagata aatcatcggc atcttcttct ggctcattag ttaattcatg tcggttacta
    26161 tcctgaaagc tgagaaaatg tatttcatca tcagtccgtt gtctgtaatg tccatatata
    26221 aaatcatatt ctttttgata agattccttc cagtaccagg ttgtatcacc acacaaccat
    26281 ggaactcttt ctgacgaatt accaacacac tgagatgagt attcagataa ctcagacctg
    26341 aaagaagtta ccatctgata aaacaagtca ggatgctttt cataatctgg tgacataaga
    26401 tcaaactccc cctgcatcca gcagacagat agcagtttat tctttctgtt atattccaga
    26461 gctaccttaa cccgggctac taagtcctca tagagtgctg ttccggtgcc ccaccgcatt
    26521 gcatcagctg ttgcccctgt atcaggtaca tacatacctt cagctccaac agtaaaggcg
    26581 gcacctcctc tgcaacaagg aaccagtaat atccctgctt gctcaggtat gtatggcagt
    26641 aactttttag ctatatgcaa tccctgtccg acacaaccat attccccttt atgcaaatca
    26701 gcagccggat ggtgatatcc actcatatcc tgaacatcat gcaggcagtg atctgccgga
    26761 ataatctcat tgaatttaca ctcttttcct cctggtgtga ttgtttttcg acgagccagt
    26821 tgttttacac gggactcggg tttgtcgagt gtatctggca ggggaatacc ttcaccgtag
    26881 gccataccat tagactgtcc tgcgagggct atcacatacc agaaacctat tttttcatta
    26941 aaatcatgat gatacatcat tcccccttta cataatagat ttaattaaat attttcatta
    27001 ttaaaagaaa gtgttaaatt aaaaactcat ttgctctaat ccatataaat caatatatgt
    27061 ccgacaacaa aaaaaccgtg ttacccgggc taacaacgac cagaaagccc attgttacaa
    27121 aaaaactttc agaaatcgtt gccgaagagc ttgaaagaat gatccgccag gaggagataa
    27181 gggaaggtga atacctgcct tctgaacgtg aacttatggc attctttggc gttggaagac
    27241 catctgtcag agaggcttta tcatctctca gacataaagg gctaatccgg tataacaagg
    27301 gagaaaaagc cagtgtctgc cgtccatcac cagaaaccat cattaatggg ttatcaggca
    27361 ttgttaaaga ttttttatct acagctgatg gcatcaagga ttttgaaaga ctacgcatct
    27421 tttttgaaac caaccttgta agatatgcag caaaaaatgc aacgcctgag gatattcaca
    27481 ggcttgagaa ggtgctcagt atccatcata actcactgag aacccccgaa gtgtatgctc
    27541 aatatgatat tgaatttcat agtatacttg ctgatatacc tgacaataaa gtattaaaaa
    27601 caatacactc agcattttct gaatggctga taatggccaa acctctggaa aagaataaaa
    27661 tgacatatga aaataacaaa aaaacccatc agcagcatat tgaaatactt acggccataa
    27721 aagagcatga ttgtgataaa gctgaacagc taatgtataa tcacctttat aaacgaattg
    27781 aagcatcata gttactcgac gcttcaccag gtagtcacca taaatattat ttaaatccat
    27841 gagaatacat ccagtccata aacagtttta tccatcctcc aacagaaaga tctgtggccc
    27901 actgaatact aaaaccatgc cctcctttct catagagatg catttcagca gaaacgcctt
    27961 tctctattag gccacggtac atgagcagac tgttttcaat tgacacaaga tcatcatcac
    28021 aacaatggag taaaaacaca ggaggaaaac atgatgtgac attaagatcc gcactatatt
    28081 gtattatatt ttctgaggta ctattgtccc ccagtaattg cttcctggag cctggatgtg
    28141 tcacatcatc tttcattgat attacaggat aaattaaccc gacaaaatcg ggaatagcga
    28201 aaatctcttg ttctttactg tcagaagaac gtttgaaatc acaaatctga ttcacaaacg
    28261 ttgaggcgat atgcccccca gcagaaaatc ctaatacacc aatacgatga cgctgtagat
    28321 gccatttatc agcattctta cgaatataaa gcattgcaga tattgcatca ataaaagttt
    28381 tgtttatatt ttctccctca actggcgatg gtaatctgta actaagaaca aataatgtat
    28441 agccctgaga taaaaactct ggagcaagag ctgtaccttc cttatctaac ataacagcct
    28501 ggtagccccc cccgggaata atcaatattc ctgttccatt tggtttatct ggtaaaaaaa
    28561 taattaactg aggagcataa atatctgtca atacacgatc aagttcaaat tccccgttat
    28621 gttcaggatc ctgataaata cggcctgaaa gagatttact ctcagatatt gtctcacttg
    28681 acaagtcaac aatagttgat ttattaaaca agcttaacat catttcattt aaagatctat
    28741 ctttattcat tataaacccc attaaaataa ctcaggtgac tgaacaacta atacagtcac
    28801 ctgcattaca gttatttaac aatctttatt tgtttgtcat tttttttcag ataaataaca
    28861 tctgaaacag ctttaccatc ttttttctcc ccccccagaa tgagcatccc cccctgccag
    28921 ggcaggctta caccataagc aagtccttcc ggcaaagaac caactttttt ccagtcattt
    28981 tttattagct gatatatctc ttttgaatag gctttatcca gtcctttatg agaccagtat
    29041 tcaccattgc tatacttttc tgccgccccc gggaaatatg caccaccggc cagaaagata
    29101 tttccatcaa taacagaggc atatgctcct gacaccccct cgggtgaagc tactggtgga
    29161 agttcagacc atttaattcg atcatgcgaa agctcccccg tccaggatct aacagaacga
    29221 agccccggtt ttctctcccc tcccagtata taaatttgct gttctgcaaa aatcaccgat
    29281 gaaccagcag ttcccggaaa tggagtcgtt cccaggttct tccactggtt ttttgacggg
    29341 tcataagaaa ttatatgatt atttaaaaaa taatcatcag caggtttact aaaataatcc
    29401 ctgttgactt tctctgtcag cgcggaattc ccttttgata actcgacatc tgaaaagtat
    29461 ccattaaaaa tattctcatt tacaccacca gtagataccg catgaccatt atggataaaa
    29521 gaaacatgac cggcaagaga tacaggggga cgggtcatca atttttccca tttatcttta
    29581 gcaatatcat aggaatagac atctttttgt aaggttatga ctccactttt atcttttcca
    29641 attcctccaa atacatagat cttaccatca ataatagtag ctgttgcctg ctctctggga
    29701 actgtcggcc attgcgctat cttagtccat tttttttcac tctggtttgt atctatcata
    29761 taccagttat ttcccagact tcctagtcct atatatattt taccattctc gatccctcct
    29821 acaccatatt taaatggttc aggaatttcc ggtaaagctc cggctaccga cgttccaata
    29881 aacaagcaga agggaagata tataaagttt ttcactttca ttgtaatcac cttttaaaaa
    29941 ttaatcagaa caccgacacg gtaactattt tcatgtttat tatctttccc cttatacacc
    30001 ccttgtttat ccaagtaatc atattctata tatggggtga acaccggtga cattttatat
    30061 tgcagaacaa atgcattttc agttgctgtt tttttatgat tcccatattt gaaattatta
    30121 accttcgtat ataccgtggt ctgccatgca aacaaccatg aatcattaat tttgtatgtc
    30181 acataaccat caaactgatg ctgatcatta cgcgccctgt tgccatctaa atccgtctgt
    30241 cggaaagctt tccagtcata gcgatagcgt aaggccagat taagatctgg cgttgcctga
    30301 tacgaaattt tcagataagg gttatactga cttcctgctg acatccattg tgtctgaatc
    30361 cctgggatta gagcccattt gtcatctatt ttatatgttt tatttatatc tattttatta
    30421 taattgagtg caaacaattc attttcattg ttgtgaattg tattccaggt gtttgactca
    30481 aaacttgccc accaaccatt attccacccc tgactaacct gaatcaacgt ttcataagcg
    30541 tgactggcgc ttctgtatcc cccacgtaga ttaagagtta atgcctgaga gcacaaaggt
    30601 aaaccaagaa gaattacagt taatagtgga taatattttt tcataaatgc ctcatcatta
    30661 aatatattta tcacttgtaa tttgatttct caatgaaact tatgttccgc aagccattcc
    30721 agaagcaggc tttgccattt ttctatcgga tatccatttt ttccggatac accaaatcca
    30781 tgacctccct tatcaaaaaa atgagcttct gcatgatgtc ccttatcccg aatagagtta
    30841 aaatatctca gagaatgaat aactggaaca tccttatcat ctgttgcatg cacaataaag
    30901 actggtggca tatactctgt aacactattt tcaagactgg ctgaatattc actttcacct
    30961 gcatccagaa gacgtttccg gcatccctga tgagcaatac tcctgtcaag agatataact
    31021 ggatatatca gagccatcca gtcaggacgc acagaagtct cttcctcata atggagatga
    31081 gtaatatatc cagcaagatg accacctgcc gaaaacccca tacatccaac actcttaaat
    31141 gttctctccg acattccggc ccagtaagaa ataaagttca gggcattctt cgcatctgca
    31201 aaggaataat aaccattaac ggagggcatt ctgtaagaaa gaataaatgc cgtatatccc
    31261 tgacctgcta tccatctgcc aacatcaagt ccctctcttt cataaacaat cctcctgtat
    31321 ccacctccag gtataatcag cacactatgt tgattagata caacaggctc aacaactgta
    31381 ataaggggca caatacctgt atatattatg ccatcgccag tattctcaaa tagcgaactg
    31441 tcagttatgt aacgctcata attatcaaga gaatatatat tctccctgta atgaatcatt
    31501 tttttattcc ctgatagcat ccgttattct tctgcaacat tcttttttct tgctccataa
    31561 aaaggttttc catcgacagt atcatattca agcgcagctt ctggatggat ccatctctgt
    31621 acacgtgatg gcatatcaaa accaataagg acaagaacaa caaatgttaa gataaaagca
    31681 agagaacaca ttgctgtacc aagacttgta tgagacgcaa caactgcacc aagaataggc
    31741 gcacatgctc caccaagtga acctacatta taaataaacc ccagtcctgc cgcacgctgc
    31801 tccacattaa aataaccact gattattttc ggcaccagag caccaatccc ctgaccaaaa
    31861 acctgctgag taaagattag aaggccaagt aaccatacat agtcccggtc cacaagaaaa
    31921 acggggataa taacaagctg accgataaga agtgatataa catatgcttt tcgggttcca
    31981 agccagtccc ccataaaacc acctatacaa cttccagctg cagagccaaa acctgccagc
    32041 ataaatatga gagcaacagt ttctggtgaa tactgcatat caaccttaag ccatgtagga
    32101 agaaaagcct gcagtggcca tccgtagagg aaacagaaca taaccacaag catcaacata
    32161 atgcctgttg gccatctttt ccccataccc tgatatataa aactgataaa aacagccgca
    32221 cagcacaatg ccataactgt gattaaagca gcgtttgcag tgacgcctga aaaacaaaca
    32281 tatagacatg caaatgcaat acaagacaga agaatattta tgtatttatt cttttctctg
    32341 tacagaatat ccaccattgt tctgacgggt ttattctctt gttgcctttt ttgccagtca
    32401 tcagactccg gaagattctt gcgaagataa aaagcaaata atattggaag catgccgaca
    32461 aagaaaagag aacgccagcc ccatagtggt actacaatgc tataaacctg tgcagctaaa
    32521 cctcccccaa tagaaaatcc agaaattaaa aaaccacttg ccttgtttct gagatgaaca
    32581 ggccagcttt cgataacata tgttacactt gagccatatt cacctgccat accaagccca
    32641 ataacaatgc gagcaataaa catcactgca tatccaggag ccagaccaca ggcaagggtt
    32701 ccgagagaga atagcactat acttaaaacc attgccatcc tgcgccccat cttgtccgat
    32761 aaagcgccaa tagcaagccc cccaaaccat cgagaaataa aagcagcgga aataaggctt
    32821 gccgcttcta ttgtcgtcaa gccgaattca tgctgaacct ctgtaagaac cagagatatc
    32881 agaacgaaat caaaaccatc aagtaaataa cctgtccatg ccgcaccgaa tatcttccac
    32941 tgtctgggtg aaagatgctt ataccaaacc tgctttttct gaatacagga gtccatatta
    33001 aacctcacaa taatagcgga tttataatac actggagtat tactttaagt aatacaatcc
    33061 cggtcccaaa gagttgcacc aataagacca gagtcacttc tgaaatatgc aggaaacagc
    33121 attacattac aaatttcaga atatatggaa agatgtttcg acactttctg aacataccca
    33181 tcagcaagtc caacacttcc tccgacaaca acaacctgac aatcaagcag taattttaaa
    33241 cttactatta attcagcaat agtactggca gaacgctctg tcaaaaattc tgcttcctta
    33301 tatccctgtc tcgctaattc aaaaatatat ttggtgctat atcctgcaag tttatttttt
    33361 gctgccccca tgatggcccg accagatgaa accgcttcaa cgcatccccg acgaccacat
    33421 ccacattctg tatccgttac tccggacaat atatgcccaa catgtccggc taatccagaa
    33481 acacctgtta ataattttct gttaactaca attccccctc caacccccgt agaaacggtt
    33541 ataaaaacca tatcacaaat ttctttcggc aaaactgtat attctgccca tgcggctgcc
    33601 tgggcatcat ttatcacact tccatttaaa ccagttatat cttccattat atttttcaga
    33661 ggatactctt tcagccctcc aagattgtct ggattcagag ctgtcaatac tccattatta
    33721 ataatccctg ttgatgctac tgcaaatcgc tccgctgttc ccttcatttc agaaaccagt
    33781 agacgtaaag cccctgtcat ttcgtcagga catctttcat gaggtgttga tatctgtttt
    33841 ttcagcaaaa atgaaccgtc atcagatatc aacgccgctg aaattttcgt tcctcctata
    33901 tcaacagcca gtgttatcat tcattttcac ctatataaca atcttgcaat tattcagatt
    33961 attttcattt ttttgtttct gtcatgttct tcgaacagtc gctctgcaag ttttgataat
    34021 acatccagat tcttttcctt taccggagaa aaaggacttc tgcatacagg agtatttata
    34081 atccccatat agtaaagaag agtttttata cctgccagga caccactctg aataagagtg
    34141 tcaataacct ggttacaggc tacttgcatt tccttcgcct taatcacatc attgtttttg
    34201 actgcttcaa atatttcaag ataacgccac cccattatat tatatgtact accgattccc
    34261 ccatctgcac ctgcaattaa ccctgatgca aatatttcat cgtaaccatt atataacaca
    34321 agctctggat gattcctttt gatttgttcc atctggaata aatcaccgga tgtctgtttt
    34381 aatgcacaaa ccctgggtat tgtgacaagc tcatttatct gatctaaaga aaaacgcact
    34441 ccgctaagag ccggtatatt ataaactacc atcggaagtc catctgcaga atcaataatc
    34501 ttcctgtaat ggatacaatg ctcttcaaat gaaaaaggat aataaaatgg agtaacagca
    34561 gatatagcat gatatccgta tttttttgca gcttttgcaa gtacctcgct ttctgccgta
    34621 ctgattccgc ccacatgcgc tataagtgtc agtcttccat ctgattcatc ggctactgtt
    34681 tcaagaattt tttctcgctc tgcaacattc tgaagaaatg cttctccagt agagcctcca
    34741 acatacaatc ccttaatatt ttgttcgata ttaaaacgga tcagttttcg aagactctcc
    34801 atatcgattt gctgggattg gtcataagga gtgggtaatg ctgatatgac ccctttaaat
    34861 tcacattgca tttttaatca ccttcactta actgcataat gggataccta gtacaccatc
    34921 aagtacaaag attatccaac agaaaagaag cacaatgtga agattatcaa aaaaaaacca
    34981 tcagaaagct gtcatgccgc tcagttaagg atcggttgat cgatccagtg tccagttact
    35041 attctctgcg taaccctctg cgactatctt gccccggaac tgattaccca cagccctgaa
    35101 gagtccgaca ctgtcacgct acgcaaacgg cgtcttcctc tggaaatgat ggcctggtga
    35161 attatcagta tggcccatga gcgtaaggaa ccttcaccag attgtgaacc ggctggacac
    35221 atgctgccag gtagtcgccc ctttgtcgcg ccgtgatcca tgcccagcag ggccaggaag
    35281 cggggccgta cgccacgtgt tcacgtaaac agcgcagccc tgacataaca ccacgctgta
    35341 tccgctctgg tgcaatatga acctgctgac catcgatggc gtgttctaac taacacagga
    35401 cacgccagaa cacgacataa agcttcactc gccagataca tggtaagcgt aaactgaccg
    35461 ccgtatgtag ccattaagcc agtattggta acgtaggtgc ccaccttttc atcaactagt
    35521 ggacacctgt tatggaacag aaaatattat ccgcagaacc ccgcagaaca ttttcaaatg
    35581 agtttaaact tcaaatggtt aaactggctt cacaactagg agcctctgtt gcccgtattg
    35641 ccagggaaca cgatatcaat gataacctcc tgttcaaatg gctcaggctc tggcagaacg
    35701 aagggcgtat atcgcggcgt cttccggtaa caacctcttc tgacgctggc gttgaattat
    35761 tacctgttga gataacgcct gatgagcaga aagagcctat ggcggctctt actccgcttt
    35821 tatccactcc ttcacaaagc acagtcagtg ccagttcctg taaagttgag ttccgccacg
    35881 gtaacatgac gctggaaaat ccttcaccag agctgctcac ggtgttgatc cgtgaactga
    35941 ccgggagggg gcgatgatct cgctcccatc agacactcga atctcgctgg ttgccggcgt
    36001 tactgatatg cgtaaatcct tcaacggtct gggtgaacag gtacaacatg tgctggatga
    36061 gaaccccttc tccggtcacc tgttcatctt ccgtggccga cggagtgaca tgattaaaat
    36121 cctctgggct gatgctgatg gtctgtgcct gttcaccaaa cgcctggagg aaggcctgtt
    36181 tatctggcct gcggtgcgtg acggtaaggt atccattacc cgctcgcagc tggcaatgct
    36241 cctcgataag ctggactggc gtcagccaaa aacatcccgt cttaacgcac tgacaatgtt
    36301 gtaaaaatgt catggccgga ttataaaaac ggtcatgagt cggaaatacc tcattcgtat
    36361 cactgagctg gaaaggctgc tctctgagca ggctgaagcc ctccgtcaga gagatctgca
    36421 actgagtctg gttgaagaga cggaggcctt cctgcgctct gcactggcac gtgccgaaga
    36481 aaagatcgaa gaggaggagc gggaaataga atatctgagg gcccagatag aaaaactgcg
    36541 ccggatgctg ttcggtaccc gttctgaaaa actgcagcgt gaggttgaac aggctgaggc
    36601 ccaactgaaa caacgcgaac aggaaagcga tcgttacagt gggcgtgagg atgacccgca
    36661 ggttccccgc cagttgcgac agtcacgtca tcgtcgtccg ttaccagcac atcttccccg
    36721 tgaaatacac cgcctggagc ctgaagaaag ttgttgcccg gagtgtggca gtgagctgga
    36781 ttatctgggt gaagtcagtg ctgagcagct ggaactggtg agtagcgccc tgaaagtgat
    36841 ccgcactgtt cgggtaaaaa aagcctgtac aaaatgtgac tgtatagttg aagcgccagc
    36901 gccgtcccgc ccgatagcgc gcggcatcgc gggctccgga ttacttgccc gcgtgttaac
    36961 gggaaaatac tgcgaacacc tgccactgta tcgtcagagt gaaatctttg cccgacaggg
    37021 tgccgaactg agccgtgcat taatctccaa ctgggttgac gcgtgctgcc agttaatgac
    37081 tccgctaaat gatgccctgt accgttacgt gatgaacacc cgcaaggttc acactgacga
    37141 cacaccggta aaagtgctga caccgggcag aaaaaaggca aaaacaggac gcatctggac
    37201 gtatgtccgg gatgaccgga atgcgggctc atcagagcca ccggcggtct ggttcgccta
    37261 ctcaccagac aggcagggaa aacatccggt acaacacctt cgtcccttcc ggggtatcct
    37321 gcaggcggat gcattcagcg gttacgatcg gctgttcagt gccaaacgtg aaggtgatgc
    37381 acagacagaa gttgcgtgct gggtccatgc ccggcgaaaa atacacgatg tatgaacgac
    37441 atctcttctg acgacatttt cctgctgaaa cagcgcctgg ccgaacagga agcgctgatc
    37501 cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa tagaccatct gcaggcgcag
    37561 ctggataaac tccgccggat gaacttcggc agtcgttccg aaaaagtctc ccgccgtatc
    37621 gcacaaatgg aagccgatct gaaccggctt cagaaagaga gcgatacgct gactggtagg
    37681 gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc gcacccgtaa gccgttccct
    37741 gaatcactac cccgtgacga aaagcgactg ttgcctgcgg cgccgtgctg cccgaactgc
    37801 ggcggttcac tgagctatct gggcgaggat a
//
LOCUS       DAOFSV010000036        36035 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.036, whole genome shotgun sequence.
ACCESSION   DAOFSV010000036 DAOFSV010000000
VERSION     DAOFSV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.036"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..162)
                     /locus_tag="QBH27_001897"
     CDS             complement(<1..162)
                     /locus_tag="QBH27_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HDQ7014210.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNT"
     gene            359..778
                     /locus_tag="QBH27_001898"
     CDS             359..778
                     /locus_tag="QBH27_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDQ7014211.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            778..2046
                     /gene="umuC"
                     /locus_tag="QBH27_001899"
     CDS             778..2046
                     /gene="umuC"
                     /locus_tag="QBH27_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDQ7014212.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(2091..2621)
                     /gene="dsbB"
                     /locus_tag="QBH27_001900"
     CDS             complement(2091..2621)
                     /gene="dsbB"
                     /locus_tag="QBH27_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDQ7014213.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2767..4308)
                     /gene="nhaB"
                     /locus_tag="QBH27_001901"
     CDS             complement(2767..4308)
                     /gene="nhaB"
                     /locus_tag="QBH27_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDQ7014214.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAALLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTVAGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4530..5249
                     /gene="fadR"
                     /locus_tag="QBH27_001902"
     CDS             4530..5249
                     /gene="fadR"
                     /locus_tag="QBH27_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDQ7014215.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5301..6833)
                     /locus_tag="QBH27_001903"
     CDS             complement(5301..6833)
                     /locus_tag="QBH27_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDQ7014216.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7163..8461
                     /gene="dadA"
                     /locus_tag="QBH27_001904"
     CDS             7163..8461
                     /gene="dadA"
                     /locus_tag="QBH27_001904"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDQ7014217.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVDQAAFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEALSVARYSRGFTPSRPGHLHGAHS"
     gene            8471..9541
                     /gene="dadX"
                     /locus_tag="QBH27_001905"
     CDS             8471..9541
                     /gene="dadX"
                     /locus_tag="QBH27_001905"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDQ7014218.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9598..11334)
                     /locus_tag="QBH27_001906"
     CDS             complement(9598..11334)
                     /locus_tag="QBH27_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDQ7014219.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11429..12343)
                     /gene="ldcA"
                     /locus_tag="QBH27_001907"
     CDS             complement(11429..12343)
                     /gene="ldcA"
                     /locus_tag="QBH27_001907"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDQ7014220.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTEEGTQLTISGHPVLKM"
     gene            12443..13054
                     /gene="emtA"
                     /locus_tag="QBH27_001908"
     CDS             12443..13054
                     /gene="emtA"
                     /locus_tag="QBH27_001908"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDQ7014221.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13056..13790)
                     /locus_tag="QBH27_001909"
     CDS             complement(13056..13790)
                     /locus_tag="QBH27_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDQ7014222.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            13991..14245
                     /locus_tag="QBH27_001910"
     CDS             13991..14245
                     /locus_tag="QBH27_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDQ7014223.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(14295..16265)
                     /locus_tag="QBH27_001911"
     CDS             complement(14295..16265)
                     /locus_tag="QBH27_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ7014224.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(16291..17145)
                     /locus_tag="QBH27_001912"
     CDS             complement(16291..17145)
                     /locus_tag="QBH27_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDQ7014225.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CISGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(17142..17954)
                     /locus_tag="QBH27_001913"
     CDS             complement(17142..17954)
                     /locus_tag="QBH27_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDQ7014226.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            complement(17964..18722)
                     /locus_tag="QBH27_001914"
     CDS             complement(17964..18722)
                     /locus_tag="QBH27_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7014227.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(18719..19699)
                     /locus_tag="QBH27_001915"
     CDS             complement(18719..19699)
                     /locus_tag="QBH27_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDQ7014228.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(19699..20721)
                     /locus_tag="QBH27_001916"
     CDS             complement(19699..20721)
                     /locus_tag="QBH27_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDQ7014229.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQHITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            complement(21051..22748)
                     /gene="treA"
                     /locus_tag="QBH27_001917"
     CDS             complement(21051..22748)
                     /gene="treA"
                     /locus_tag="QBH27_001917"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDQ7014230.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQSWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
     gene            complement(23068..24486)
                     /gene="dhaM"
                     /locus_tag="QBH27_001918"
     CDS             complement(23068..24486)
                     /gene="dhaM"
                     /locus_tag="QBH27_001918"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDQ7014231.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGTPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(24497..25129)
                     /gene="dhaL"
                     /locus_tag="QBH27_001919"
     CDS             complement(24497..25129)
                     /gene="dhaL"
                     /locus_tag="QBH27_001919"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDQ7014232.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFGKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(25140..26210)
                     /gene="dhaK"
                     /locus_tag="QBH27_001920"
     CDS             complement(25140..26210)
                     /gene="dhaK"
                     /locus_tag="QBH27_001920"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDQ7014233.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRLWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            26438..28357
                     /gene="dhaR"
                     /locus_tag="QBH27_001921"
     CDS             26438..28357
                     /gene="dhaR"
                     /locus_tag="QBH27_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDQ7014234.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(28394..28570)
                     /locus_tag="QBH27_001922"
     CDS             complement(28394..28570)
                     /locus_tag="QBH27_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014235.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            29649..31406
                     /locus_tag="QBH27_001923"
     CDS             29649..31406
                     /locus_tag="QBH27_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Ec67 family RNA-directed DNA
                     polymerase/endonuclease"
                     /protein_id="HDQ7014236.1"
                     /translation="MFKPTYLARLQACCNKFELADLLQIKVTFLTNVLYRIRPENQYK
                     KFTIKKKSGGEREIFAPDEKLKDIQQRLSELLYICQEEIWAKNNIKQNVSHGFEKNKT
                     IITNAERHRDKNIVFNIDIENFFPSFNFGRVRGYFIANQNFKLHPNVATIIAQIACLD
                     GSLPQGSPCSPVITNLICRILDFRLSKLAVTYGCSYSRYADDITFSTNKKNIPDALVS
                     NEKENEPGKILVEEIHRAGFTLNHNKNRVSRCTSRQQVTGLTVNKKINVSREYIKNTR
                     AMAHSLYFEGSYTLIEKDGKHRKGTLSELEGRFAFIDMLDKYNNVEAKKNARPERYVV
                     KGFGLDFKQRLNSREKAYSKFLYYKNFYGNEQITILTEGKTDPVYLKCAIDSLFLDYP
                     QLVREEKNTKNRVLKVNLFKTNDKKKYFLDLSGGAADYSRFFRRHGLLCKAYEKQPPK
                     NPVIILLDNDTGPSDFINQIIKDYSHLPKKAEDVRKGAFYHLESNLYVLFTPLLPGDN
                     YSSLEDFFEPKVLQMKYNGKSFDKSNNHDSSTTFGKDRFATYIVRENRKTIDFSLFKP
                     ILDSIIEIKKHFINLHPSK"
     gene            31653..32780
                     /locus_tag="QBH27_001924"
     CDS             31653..32780
                     /locus_tag="QBH27_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014237.1"
                     /translation="MVEKMKQNSSSTLGALSAPTTWPGANARLLGLGVGLPIAPLDRL
                     AQFSAAEFERFTLEWASEYLAGQQYVNEVQQRGGAGDKGRDIVVWLDPSDAVPRRWHL
                     YQCKHYDANLGLPKAGIEVAKVIYYTFIKDYTVPTQYHFVTHKGVTSPFQDLLDDPTK
                     LKEKMLSEWATFSKQITSKHSVDLTPELEKYIKEFDFSIFHAKQPIEILAEHSKTSFH
                     LMVFGAPLIERDPPTRPPSSVAPIETVYIEQLFSVISADIKTNVRDLVDFQSSISHVK
                     LFERSRITFYCSEGLKELARDQMANQEFFNSLLVEFDDGLYHYTSDLTGTPLLRLKNT
                     VKAAQTLQLGAHPLAIHVTNKDREGICHQLANTNLINWCNP"
     gene            32777..33292
                     /locus_tag="QBH27_001925"
     CDS             32777..33292
                     /locus_tag="QBH27_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014238.1"
                     /translation="MSEMQVFEGNFEQRPFNTPLEYGFRALFILDAADNVAMDLQRIV
                     SYDYLLVHTSDVPRGPKSLHPAVPHRGAELLVKRSAIQAGLHLMLSRELIKVVFAAEG
                     ILYQATNLTGRFVRLLMSQYSKELAERASWVTKQFYEYTDEELASYISQNVGQWGSEF
                     DRLTAIDLLDL"
     gene            33294..35180
                     /locus_tag="QBH27_001926"
     CDS             33294..35180
                     /locus_tag="QBH27_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014239.1"
                     /translation="MLRIRKILLRGNSVQDAYVDFYKGANILAGESDTGKSYLVSCLD
                     YILGAEKLKKKLKEATDYTHLYVEFENDEGGVLTLKRGLEGGKLEAHDVAIQDIHGEG
                     KIIAPVRKGTSKGPDVTSILFPFAGIKEAKLRKNARGETQRFSIRTLAPIFLVDEVSI
                     IDEYSPVTGRSGYDDTARKRMFSYILTGHDDGGVTVEEKPEIVKARLMAKLEFIQDLI
                     RPLDERFSICSPKFPLTSSADDLSDQLIAQAIDEVERAAAAISDLLEGIKMETALTLK
                     IESQLMGVSEIQSRYSLLEERYHSDLKRLDFISEGSHYFTSLQEVPCSLCGQNLLHPH
                     SENAKKLMNSNEVRRSSLAEAAKIHGYLAGLQKAMSDLDRRKEALNIDRYKSKESLDG
                     MKNQIKYTFEPLLTLSKESLDLAIEQRAERDSELNDRQRWFELLKHQKDIEILLEKSS
                     QPRLEWEGLSTRTLAELCREIETVLKDWKWGAEPDVSFNEKEYDIIVDGQPRQSHGKG
                     VRAILYSAFIIGLLKYCIKKGYPHPGLVVIDSPLTSYKKKAAATVSGFDEPISAGIEE
                     GFWHSLTQLPDTLQIIVIENKEPPVSIMNKVHYEWFAGEHAMDGERRALIPLPSKNSA
                     ILMK"
BASE COUNT         9395 a         8527 c         9108 g         9005 t
ORIGIN      
        1 cgtgttagac agggtggtaa agaaactctg cacagatttt aacttgttct tcaattctgg
       61 aatcagtttc ccttcaacta cgcccgcagc aatagaacac agtttcttga tgccgtcatc
      121 cagtacctta atgagaatgc ctttctgggc gaatgcttcg attctatttt aaaccaggtg
      181 atatttttcc tttctgctgg atggacgctg tcgccagaag gccgcgtgag cacaggataa
      241 gagaacgaaa aatcagcagt ctatgcagcg acaaatattg atagcctgaa tcagtattga
      301 tctgctgaca agaacagact actgtatata aaaacagtat aacttcaggc agattattat
      361 gttgtttatc aagcctgcgg atctccgcga aattgtgact tttccgctat ttagcgatct
      421 tgttcagtgt ggctttcctt caccggccgc agattacgtt gaacagcgca tcgatctgaa
      481 tcaactgttg atccagcatc ccagcgcgac ttatttcgtc aaagcaagtg gtgattctat
      541 gattgatggt gggattagtg acggtgattt actgattgtc gatagcgcta ttaccgccag
      601 ccatggtgat attatcatcg ctgctgttga cggcgagttt acggtgaaaa aattgcaact
      661 acgcccgacg gtacagctta ttcctatgaa cagtgcgtac tcgcccatta ccatcagtag
      721 cgaagatacg ctggatgtct ttggtgtggt gatccacgtc gttaaggcga tgcgctgatg
      781 tttgccctct gtgatgtaaa cgcgttttat gccagctgtg agacggtgtt tcgccctgat
      841 ttatggggta aaccggtggt tgtgctatcg aataatgacg gttgcgttat cgcccgaaac
      901 gctgaggcaa aggcgctggg cgttaaaatg ggcgatccct ggttcaaaca aaaagatctg
      961 tttcgtcgct gtggcgtggt ttgctttagc agcaattatg agctttacgc agacatgagt
     1021 aatcgggtga tgtcgacgct ggaagaacta tcgccccgcg tcgagattta cagtattgat
     1081 gaggcattct gcgatctgac aggtgtgcat aattgtcgcg atctgactga ttttggcaga
     1141 gaaattcgcg caacggtgct acaacgtacc catcttactg ttggtgtggg gatcgcccag
     1201 accaaaacgc tggctaagct tgccaatcat gcggcaaaaa aatggcagcg gcagacgggt
     1261 ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat taatgtctgc tctccccgtg
     1321 gatgaagtct gggggattgg acggcggatc agcaaaaaac tggacgcgat ggggatcaaa
     1381 accgttctag atttggcaga ttcagatatc cggtttatcc gtaaacattt taatgtcgtg
     1441 ctcgaaagaa cggtgcgtga actgcgcggt gaaccctgtt tgcaactgga agagtttgca
     1501 ccgacgaagc aggaaattat ctgttcccgc tcgtttggtg aacgcatcac ggattatccg
     1561 tcgatgcggc aggccatttg tagttacgct gcccgggcgg cggaaaaact tcgcagcgag
     1621 catcaatatt gtcggtttat ctccacgttt attaagacgt caccatttgc gttcaatgaa
     1681 ccttattacg gcaatagcgc atcggtaaaa ctgctgacgc ccactcagga cagcagggat
     1741 atcattaacg ccgctacgcg atctctggat gccatctggc aagcgggcca tcgctaccaa
     1801 aaagcgggcg tgatgctggg ggatttcttc agtcagggag tcgcgcagct caatttattc
     1861 gatgacaacg caccacgccc cgggagtgag caattgatgg cggtaatgga tacgctgaat
     1921 gctaaagagg gcagaggaac actctatttt gccgggcagg ggatccagca acaatggcag
     1981 atgaagcgtg ccatgctttc accatgttat acaacgcgaa gttctgattt actgcgggtc
     2041 aaataaacat agcggcagga aaaagcgctc ccgcaggagc gccgaatgga ttagcgaccg
     2101 aacaggtcac gttttttcgc tttaaacggc tgggaaatca ccaccagcac cgcgacaatc
     2161 aggtaagcga taaaaatacc gagcagccac tgcggcattt ccagacctaa aaattcccac
     2221 tgacgctcgg cgcaatcgcc agaggcgaca aacacttgcg gcacccattt atccagcggc
     2281 agccattccg ggaaacgagc cataaaatca caggtcgcaa acggcgaagg atagagctga
     2341 agcatggtgt gctcgtaagt taactgcaca ccgcggaacg cactatacaa ccagataacc
     2401 atcgctacat aacgcagcgg agttttcggg gcgatcgcgc caatcagcgc agcacccaga
     2461 acgccgaata acgcgcagcg ttcataaata cagagcacgc aaggtttcag taacatcaca
     2521 tgctggaacc acagcgccgt cagttccagt gccagagcag taaacgccat caacagccat
     2581 gcgccccggc cttgtgaaca ttggttcaaa aatcgcaaca taatcatttc cctgcaatat
     2641 gcatagagtg cgcagtttaa accaattcat tcgctgcgcc accaggaggc agcaaaaata
     2701 ggacgcaatt ggacaattat cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag
     2761 ttgttatcaa agcgttgcta tccagcccat ttgcataaac cattcggtta caggagcaag
     2821 cgtaaactcg acgcagagta acccggcaac cgtcagtacg agggtgtaag gcagggccat
     2881 ccacaccatg cggccataag agagacgaat caatggcgcg agtgcagagg tcagcaggaa
     2941 caggaacgca gcctgaccgt tcggcgtagc gacggagggc agattggtac cggtattaat
     3001 ggcgaccgcc agcagctcat attgcttcaa cgtgatagcg ccactttcca ttgccgcttt
     3061 cgcttcgttg atataaatcg tccccacgaa gacgttatcc gaaatggatg acagcagacc
     3121 gttgaaaata tagaacaacg acagctgagc atgttcagat gcctgcaaca caaactgaat
     3181 aatcggcgaa aacagttgtt ggtcgataat caccgcgact actgagaaaa acaccgtcaa
     3241 cagtgcggtg aatggcagag attcggtgaa ggctttaccg atagcatgtt catcggtaac
     3301 accggtcaat gatgtcgcca gaataatgac tgacaaacca atcaaaccaa cttccgccag
     3361 atgcagtgcc agcgcagtca ccagccagac gccgataatc gcctggacaa tcagacggat
     3421 tttatcctga cgggtacgct ggttgcggct ttgatcgtca aactgttgca gtacttcgcg
     3481 gactttctcc ggcagcgttt caccgtagcc aaaccaacgc agcttctcta ccagcaggca
     3541 ggttaacagg ccacaaatca gaaccgggac ggtcaccggc gacatgcgca ggaagaaatc
     3601 gccaaaatgc cagccagccg ctttagcgat gatcaggttc tgtggttcgc ccaccatggt
     3661 catcacgccg cctaatgcgg tgccgacacc ggcatgcatc atcagactac gcagaaagcc
     3721 acggaactgt tccagaacca ctttgtaatg cttgtcgata tgactatcgt cttgcaggtc
     3781 ggtatcttca gtacgggaag aggctacgcg gtgataaata ccataaaaac cgactgcaac
     3841 gctgatcacc accgccacga cggttaaggc atcgaggaac gcggagagga gcgccgccgc
     3901 cacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg cttaacagca aacgggtaaa
     3961 tatgaacaac aacagctgtt tcataaaata gatacccgcc accataaaca tcagtaacag
     4021 caagacttca agatttgccg ccacttcttc acgaacatgt tccgcgctgg tcatgccgat
     4081 gaataccgct tcgatagcca acagaccacc ggggagcagc gggtagcatt tcagggccat
     4141 cgccagagtg aaaataaatt ccgctaccag caaccagcca gcgacgaaag ggctgatgag
     4201 gaaaattaac ggatttacga ttaagaaaat gatgagggcg agtttgtacc agtcggggga
     4261 ctggcccaaa aaattgcgcc atagtgcgcg gccccaggag atctccatga tggtttccct
     4321 taccttagaa ataatcaatg atgtttttat gtttaaacgc aaagcttaac ggtcagacag
     4381 gagtgaggca agtcttgata gtcaagggga aagagatgca gaaaatgaag ccttgatccc
     4441 ttttttcttc tttttgtctt ctatcagcgt agttagccct ctggtatgat gagtccaact
     4501 ttgttttgct gtgttatgga aatctcacta tggtcattaa ggcgcaaagc ccggcgggtt
     4561 tcgcggaaga gtacattatt gaaagtatct ggaataaccg cttccctccc gggactattt
     4621 tgcccgcaga acgtgaactt tcagaattaa ttggcgtaac gcgtactacg ttacgtgaag
     4681 tgttacaacg tctggcacga gatggctggt tgaccattca acacggcaag ccgacgaagg
     4741 tgaataattt ctgggaaacc tctggtttaa atatccttga aacactggcg cgactggacc
     4801 acgaaagtgt gccgcagctt attgataatt tgctgtcggt gcgtaccaat atttcaacta
     4861 tttttattcg caccgcgttt cgtcaacatc ccgataaagc gcaggaagtg ctggcaaccg
     4921 ctaatgaagt ggccgatcac gccgacgcct ttgccgagct ggattacaac attttccgcg
     4981 gcctggcgtt tgcttccggc aacccgattt acggtctgat ccttaacggg atgaaagggc
     5041 tgtatacgcg cattggtcgt cactatttcg ccaatccgga agcgcgcagt ctggcgctgg
     5101 gcttctatca caaactgtcg gcgttgtgca gtgaaggcgc acacgatcag gtatatgaaa
     5161 cagtgcgtcg ctatgggcat gagagtggcg agatttggca ccggatgcag aaaaatctgc
     5221 cgggtgattt agccattcag ggacgataat cccttccgtt taaaaagcaa acccctcaaa
     5281 cgaggggttt ttcgttgttt ttacagattt cccattctcg gcgggcaacg ttccagcaac
     5341 tcgatgctgc cgtcttcgtt ttgctgttcc agcatcacat caaatcccca caggcgatgc
     5401 acatgcttca gcacttcttt gcgcccccga tccaacggtg cgcgattatg tggaatgtaa
     5461 cgcagcgtca gcgaacggtc accgcgcaaa tccacgttcc agatctgaat attcggctcc
     5521 agattactta agttatattg cgacgataac cggttacgga tttcccgata accttcttca
     5581 ttatgaatag cggaaatctc cagataatta tgtcgatcat catccagtac ggtgaagaag
     5641 cggaaatcac gcatcacttt cggtgacagg aactggctga taaaactctc atctttgaaa
     5701 tcacgcatcg cgaaatgcag cgtttccagc cagtcggaac cggcgatatc cgggaaccag
     5761 tatttgtctt cttccgtcgg cgactgacaa atccgtttaa tatcctggaa catggcgaac
     5821 ccgagggcat atgggttgat gccgctgtac cacgggctgt tatagggcgg ctggaagacc
     5881 acattggtgt ggctgtgcaa aaactccagc ataaaacgtt ccgttacttt cccttcatca
     5941 tacagatggt taaggatggt gtagtgccag aaggtcgccc agccttcgtt catcacctga
     6001 gtctgttttt gcggataaaa atactggctc accttacgca caatacgcag gatttcacgc
     6061 tgccatgatt ccagcagtgg cgcatttttc tccataaaat agagcaggtt ttcttgtggt
     6121 tcggacggat agcggcgcgc ttcagcaacc gttttctctt cctcgcgctt cggcagggta
     6181 cgccagagca tattgacctg actttgcaga tactcttcgc gactcttctg ccgggctttc
     6241 tcttcttgca gcgagatttt ttgcgggcgt ttgtaccggt ccacgccgta gttcatcagc
     6301 gcgtggcacg agtccagaag ccgttctact tcatcaacgc cataacgttc ttcgcactcg
     6361 gtaatatatt tacgggcaaa aatcagataa tcgacaatcg aactggcgtc ggtccagcta
     6421 cggaataagt aattgttttt gaagaaagag ttatgcccat agcaggcatg agccatcacc
     6481 aacgcttgca tggtaatggt gttctcttcc atcaggtaag cgatacacgg gttagagtta
     6541 atgacgattt cataggccag tccttgctga ccgtgcttat acagccgttc agtctcgata
     6601 aactttttac cgaatgacca gtgcggatag ttaattggca tgccgacgct ggagtaagca
     6661 tccatcatct gttctgaggt tatcacttca atctggtgcg ggtaggtatc cagccggtag
     6721 agtttcgcca cccggtctat ctctgccaga taaacatcca gcaggtcgaa cgtccagtcg
     6781 ggtccatcgc tcaaacgtgt ggtgtcctta ttcatagaat cgatcgtcgc catacgcgca
     6841 cctcattgtt gtcggcgctc tctgtgtgga gcacctcatt tcaagcatag aacacctgtt
     6901 aaaaaccgcg tcgccggaga atttttttct ttgcgatttc ttattatcag agtgccacta
     6961 atccgcttct gaacggaatt ttatgctgga taaaaagggc gttcagcagg agatactaaa
     7021 gacgccatat tgccgcagag tcagggagat gtgagccagc tcaccataaa aaagccgtat
     7081 gttgaataat attttcaact gagttatcaa gatgtaatta gattattatt cttttactgt
     7141 atctaccgtt atcggagtgg ctatgcgagt tgtcatactg ggaagtggtg tggtaggcgt
     7201 tgccagcgcc tggtacttaa atcaggcagg acatgaggtc accgtcattg atcgggagcc
     7261 gggggccgcc ctggaaacca gtgccgcgaa tgccgggcaa atctcccccg gatatgctgc
     7321 accgtgggcg gcaccaggtg tgcctttaaa agcgattaaa tggatgttcc agcgccatgc
     7381 gccgctggcg gttcgtctcg acggtacgca gttccagttg aaatggatgt ggcaaatgtt
     7441 acgtaactgc gacaccagcc actacatgga aaacaaaggg cggatggtac gtctggcgga
     7501 atacagccgt gattgcctga aagcattgcg cgccgaaacc aatattcagt atgaagggcg
     7561 tcagggtggg acgctgcaac tgttccgtac cgaacaacag tatgaaaacg cgacccgcga
     7621 tatcgccgtg ctggaagatg ctggcgtacc gtatcagctg ctggaatcca gccgcctggc
     7681 ggaagtggag cccgcgttgg cagaagtagc gcacaaactg acgggcggcc tgcaactgcc
     7741 caatgatgaa accggagact gccagctgtt tacccagaat ctggcgcgga tggcggagca
     7801 ggcgggggtt aaattccgct ttaatacgcc cgttgaccaa ctgctttgcg acggcgagca
     7861 aatctacggc gtgaagtgtg gtgatgaagt gattaaggcc gatgcgtatg tgatggcgtt
     7921 tggttcttac tcgacggcga tgctcaaagg cattgttgat attccggtgt atccgctgaa
     7981 aggctactcg ctgaccattc caattgcgca ggaagatggt gcgccggtat ccactattct
     8041 tgatgaaacc tacaaaatcg ccattacccg tttcgataac cgcattcgtg tcggcggaat
     8101 ggcggaaatt gttggtttta acaccgagct gttgcaaccg cgtcgtgaaa cgctggagat
     8161 ggtggttcgc gatctctatc cacgcggcgg tcatgttgat caggcggctt tctggactgg
     8221 cctgcgcccg atgacgccag acggcacgcc ggttgtcggg cgtacacgct ttaaaaatct
     8281 gtggttgaat accggtcacg gcacgctcgg ctggacgatg gcttgcggtt ccggtcagtt
     8341 gttaagcgat ctgctctcgg ggcgcacgcc agcgatcccg tatgaagctc taagcgtagc
     8401 gcgctacagc cgtggattta cgccatcacg cccgggccat ttacatggcg cacacagcta
     8461 aggaaacgag atgacccgtc cgatacaggc cagcctcgat ctgcaggcat taaaacagaa
     8521 tctgtccatt gtccgccagg ccgcgccgca cgcgcgcgtc tggtcggtgg taaaagcgaa
     8581 cgcttacggg cacggtattg agcgtatctg gagcgcgctc ggggctaccg atggctttgc
     8641 attgcttaac ctggaagagg caataacgtt acgtgagcgc ggctggaaag gaccgatcct
     8701 gatgctggaa ggatttttcc atgctcagga tctggagatt tatgaccagc accgcctgac
     8761 cacctgcgta cacagcaact ggcagctcaa agcactgcaa aatgcgcggc taaaagcacc
     8821 gttggatatt tatcttaaag tgaacagtgg gatgaatcgg ttgggcttcc agcccgatcg
     8881 cgtgcttacc gtctggcagc agttgcgggc gatggcgaat gttggcgaaa tgaccctgat
     8941 gtcgcatttt gccgaagcgg aacatcctga tggaatttcc agcgcgatgg cgcgtattga
     9001 gcaggcggcg gaagggctgg agtgtcggcg ttcgttgtct aattcggcgg cgactctgtg
     9061 gcaccaggaa gcgcattttg actgggttcg gcctggcatt attttgtatg gtgcttcgcc
     9121 gtccggtcag tggcgtgata tcgccaatac cggattacgt ccggtgatga cgctaagcag
     9181 tgagattatt ggtgtccaga cgctaaaagc gggcgagcgt gtgggctacg gcggtcgcta
     9241 tactgcgcgc gatgaacagc gaatcggcat tgtcgccgca ggatacgccg acggttatcc
     9301 gcgccacgcg cctaccggta cccctgtttt agtggacggc gttcgcacca tgacggtggg
     9361 gacagtctcg atggatatgc tggcggtcga tttaacgcct tgcccgcaag cgggtattgg
     9421 tacgccagtt gagctgtggg gcaaggagat caaaattgat gatgtcgccg ccgctgccgg
     9481 aacggtaggc tatgagttga tgtgcgcgct ggcgttacgc gtcccggttg tgacggtgta
     9541 acttgttatc gctggatgcg accagcgtcg tatccagcgt tacgaaacac attaaactta
     9601 agattcggct tcttcctccg ctacccgaac accaatcttc aacacttcat tatcttcttt
     9661 ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa ccggtgccgc
     9721 gcctaacaac tgctggacga tttcacccag cgtttgctgc ttatcgcgat attctcgccc
     9781 gtcttccaga ccatatatca gcgccacatc agcatatttg gcgctggctt cgagaatgaa
     9841 gtcaccaaag aagcgttgat ccagcgcgac cggcggcgac tggctgaaca gtttaccgag
     9901 cgccgggaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt
     9961 gctgccggtg ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt
    10021 tggcatatgc aaatcacgca gtgccgcgcc cacgcaccat ttatcggcac tcaattgata
    10081 aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg acaccggacg
    10141 tcccactggc ggaacgacca ctttggcttt tttcgccgcc cacgagagtg atgttccctg
    10201 caatagcagt gaaaccagaa ccacaaagaa ggcgacatta aagaacagtc gcgcattctc
    10261 cagccccgcc atcatcggga acacagccag aataatcggc accgcgccgc gtaatcctac
    10321 ccagctgata aacacgcgct cacgcagatt gaagccacgg aacggtagca atccggcaaa
    10381 taccgaaaga ggacgggcga agaaaatcat ccatgcggac aaaatgagcg ccggaatggc
    10441 aataggcagc agatcgcttg ggttaaccaa tagccccagc accaggaaca tggcgatttg
    10501 cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg
    10561 attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt
    10621 ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata atccggcggg
    10681 cagggcgatg cgattaatca tttgcagcag taaataacca ccgccaagcc cgatgacaat
    10741 cccgaggcca aattgctgca gaatatcgac cacgaacatc cagctgacac tgctttcatg
    10801 ctgctggatc atcgcaatca gagtaatcgt cagaaagacc gccattggat cattactgcc
    10861 ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac ccagcagaga
    10921 aaagaccgct gcagcatcgg tggagccgac gatagcgccg attaataatc cttcaatcaa
    10981 atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga taagtacgcc
    11041 cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg cctgagtgcg
    11101 catcccgccg tcgagcaaaa taatcgccag cgccaggtta cttaccatgt aggcgaaggg
    11161 gtaattatca aacgggatac cgccgacgcc atcaaccccc gccaacatgc cgatcgccag
    11221 aaaaataacc agaataggaa tgccaagacg ggaagaaaat gaactaagta atatactgct
    11281 ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg
    11341 gttactcctg attacgctgt ctctgatata aaccctacca tattagcggc agagacagcg
    11401 ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg
    11461 tcaactgagt gccttcctcg gtgttattca gaatggcatg tgcgcccaac ggcagcgtaa
    11521 ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac
    11581 gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcatta ggtgtgctgc
    11641 cgctaaagct accgagaata atcgccttct gacgtggcaa aattccagca tgatagagct
    11701 gcaacagcat gcgttcgacg cggaaagggt gctcgttaat atcttccagc accagaatac
    11761 cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc gcaagattgc
    11821 ctccccacag cgtgccttcg gtctgacatg tcgggccttc cccttgccat tcaatggtga
    11881 acgttttatt gcgtaacgcc agccagaagt ggtgctcggt aaaggcattt agttcatccg
    11941 cgccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct tgcgccagaa
    12001 gaccgcactg aatggcggta aaatcgctat gtccgcaaat gagtaacggg tcatgttgtt
    12061 ggcgagccac cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc
    12121 cgcgtacagc cagcacgatg gtgttggggg tagtcagtct ggcaagagaa ttgagatctt
    12181 ccagacgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt
    12241 tattgacctg atgccccgcg tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt
    12301 taatgcagta acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac
    12361 aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt
    12421 ccaggctata aggatagaag aagtgaaatt gagatggttt gcctttttga ttgtgttatt
    12481 agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga aagttccggt
    12541 gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga
    12601 tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga acgcggtgag
    12661 taaatcgaat gccattggtt tgatgcagat aaaagcttca acctccggac gtgatgtata
    12721 tcgtcgtatg ggctggagcg gtgagccgac gaccagcgag ctgaaaaatc cggagcgtaa
    12781 tatttcaatg ggagcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga
    12841 agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg caggtgcgct
    12901 gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atctggatgc
    12961 tgatgagttc ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc cgcgctatat
    13021 ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cacgcacatt gtccgctttt
    13081 ttccgggctt ctcgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg
    13141 ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt tttgccatca
    13201 atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac accccattgc
    13261 cccatgttga cttcaatctg agcgaagcgc atgccttcgt gtaattcggc aggctttgct
    13321 gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta
    13381 ctgttatccg ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggtgatgcgg
    13441 aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtaat aaatgccgga
    13501 agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt cgcaccctga
    13561 gtttcggcgg taatggtaat gtgctgcgcc tttagcacgg cgatgttgtc ttcggcctgg
    13621 ctgccgaaat ccagcaccag tttatccgga gttattgcca gtattttgct gatcagctgc
    13681 ccgccattcc agctgatacg caaaggaatt gcggctttgt tcaaatcgcg taacacgccc
    13741 aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac
    13801 tcctgactga caaactgtct ttggtttatc ggtcgcagtt aacaaaactt aatacaaatg
    13861 cgcgaatatt ttttacatgt tgttcttaaa tcagccgaca tatgcccagc atcgatcccg
    13921 cccttgccta tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga
    13981 gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg gcattatcgc
    14041 caagctaatc atgccggggc gagatggtgg tggatttttc ctgacctgta ttcttgggat
    14101 agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccatcag
    14161 cggttttaat ttgcacagct tcctggtggc ggtggtgggc gctatcctgg tgctgggcgt
    14221 attccgcctc ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc
    14281 tatccgcttt cacatcagaa cgtatactcg atacctgccc agaaattgcg accttcttca
    14341 ataaaccctt cactgtaggc atatttggta tcaaacaggt tattgaccga cgcattaacg
    14401 ctgaagccat gacctaaggt gtaatcggct cgaatgtgag tcaccgcaaa accggcggct
    14461 ttttgtgaac cgtcactgtt gctgtagctg gaggaacgcg cctcttccga cagcgttacg
    14521 cttaacggct cccacggttt gagagtcatc catgcggtca ttgtctgcgt tggcagatcg
    14581 gttatcttgc cgatgtcttt gcgtttggca tcagcgtgga tcagggcgta gctcaatcct
    14641 acatccagta tattgctgat tttcccctta ataccggcat ccagaccgct gtaatccacc
    14701 gtgccgctgt tctgattttg aatggtatcg gcatcgatat tgtgcgagag gatggcatca
    14761 ctcacccggt tatagtaaac gctgacctca aatccccagt cgtgcgtgaa ggcaccattc
    14821 caggttaaat ccaccccgcg cgcgcgttcc ggcttgagct gcgggttaac aatcgctatc
    14881 tggttatacg caggtttgga cgtggtatag cgttctttca gcgtcggaaa gcgtgtgcgg
    14941 tcatagtacg aaagcgccag cgtgtcttca ttggcaaagt ggtatttccc catcacctgc
    15001 cagttaaaag ctgactgatt gttgtcgtca taatgggtga tactgccatc tttctcatgt
    15061 tttttcgctt ctacgctatc gcgccagtca tagctgattc cagccacgac atcgacatta
    15121 tcggcagcag cccattgata ttcactggcg agcgaccagg tacgatcttc atagcgatcg
    15181 taagcggcgt gcggcgcacc tttttcccgg tgtacgtcat ctttccagtt aacggcaaac
    15241 gacagcagat cgttttcacg cacatcggct gccagttgta acccggcacc gtcgctgtaa
    15301 tcggaataat ggctgtagct gcctttttta tttttcaaat cagccagcga gttgtacatc
    15361 attagcgtat tttcaaaggt gtcgcgatac agccgacttt tcagggtaaa acgatcgttt
    15421 agttgggtcg ttccctgata ataaaaactt tctttgtcat actctggcca ctgccagtag
    15481 cgtgatttct gaccactatt tccactgtat ggcgggttat ctttttcacc atcctgctta
    15541 atgtaagtca atgtgtattc atcgttttcg cgtggtgtaa aaccgagctt cacaatgccg
    15601 cgtttatcat cagccgatga attaatcatc ttgccgtgtt tgcctgcaat atcattattt
    15661 acaccatgcg gcaggccgag aaaatcctgc tttagctggc taccgctgac ttgcaaatac
    15721 cccagatcgc tgctggcggc aaatgaagca tgcatatcgt aggcattgtc ctggctacgg
    15781 ctccatccct ggcgatatcc cagacttgct tccagaggtt ttgttggctt ctgggtggtg
    15841 atattaatgg ctccgcccat ctgattaggc ccctgaagca gcgacgaata ccctttggaa
    15901 acttcaactg cccccaaatt gttggtcaga atccgcgcca gatcgaggtt gccgtcatag
    15961 ggaacataaa tgggcacacc gtcgaaatag accggcacct gacgactatc aaagccacga
    16021 actttgacct gttcttcgtt gcggctacct gacttttgca gcaccacgcc ggggacgaca
    16081 cttaatgcct gagcgacatt ctgtttatcc agggccttca tggtgggttg atcgagaacg
    16141 gtggtcgtcg ttgatgatac cggactggac catacggtca gcgtgtcact ctcctgagcc
    16201 gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt
    16261 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgcgggtt gcagactgac
    16321 tttaatgtca gctggtgtcg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca
    16381 gtcgaggtaa tttttgagtg ttgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    16441 gggagccagc gacggtgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    16501 gagctgaagt acgtctggtt gcgcgcgtaa cgcggcgatt gcctctttcg gcgtgtcggc
    16561 ttcaacaact attttcttct cgggtgcgtg gcgacgtaac tgattgattg cgcctgacca
    16621 gtcctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca
    16681 tccggcacga tgaatcagtc ctccggcagc cagaattgcc tgcgaggcca gtaagcgggt
    16741 gcccggaatt gcttttcggg tgcaggcgat attgccatca gggtaacgtt cacgaagtaa
    16801 cgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    16861 tgccttccat ccctgatgaa gtgctgcggc attaccttgc gcacggatta gccgctgacc
    16921 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    16981 catcttacac gcgacagaaa tgccgctgat gcagccgccc tgacggagaa aaaactctat
    17041 atagccatgc tggtgtccaa tatttaatgc tcgcgtggtc aggtcaccgc cctggatatc
    17101 ttccagcaat aacgcatcga tttgtgcctg agaaaggaaa atcatcgcag tgtctcctgc
    17161 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    17221 tcttgttgtt gataccagcg atataaccgc tcacgctcat cgtcgttaag cgcgcccaga
    17281 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    17341 ccgcggataa aatcaacgtg agcataaatt cccatctgat acaggacgtt gagtgcaaag
    17401 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    17461 aacgaggtgc tgaccagatg agtcgtatag acgcgaagcc gtgcctgatt attcagtttg
    17521 ctcattgcct ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    17581 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtctgcc
    17641 tttagcgcgg cggcacgacg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    17701 taaatcgtcg tgagtttgtc cgccagcgcc agcgatacgg tacccggtcc gcagcccata
    17761 tcgaataagg tttgcgcgcc ttgcaggtca atttttgagg tcagttgttg caggtagctg
    17821 tccgtaggcg aggcgcaatt ttccgccatt ttttccgccc gttgatccca gtgatccggc
    17881 gttttttctg tgcgatgggc cagcctgagt tgctgaaggt aaaggtctgc aaaatcaata
    17941 tcgtcaatga gcatgatttt tccttagtgc ttaattgcag agaggtgatg gtgaatctgg
    18001 tcggcagaaa cccggtacag ggcagccagt tttttcgttg ttaactgttc ggtcggtaag
    18061 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgtttgca
    18121 tggagcggat gatgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    18181 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcaggctc atccagtaat
    18241 aacaagcgcg gttgctggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    18301 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    18361 gttgctttca ggcgttcttg cttaccggga acggaaaaag cgcctatggt gggggccagg
    18421 cccatcaaca ccatatccag cacactaaaa gcaaaaatgc cgtcgtgcgc ctgcggcacc
    18481 caggcaatag cctgcgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    18541 aagatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    18601 ccgcaaccat tggctcctaa cagacaacaa attgttcctt gcgacagggt gaaggacacg
    18661 tcgcgtagca ccggatggcg ggagtaaccc agagacgcat gacggacggc aagcaaagtc
    18721 actggcgacc tccgcgtaac aacaaaaaca ggaaaaacgg tgcaccaaca aaagcggtca
    18781 gaatgccaag tgggatttct gttgttccaa tgctgcgtgc cagcgtatcg gtaagcagga
    18841 gcagaatcgc gccagtacac attgccatgg gcaacagctg ctgatggtta tgaccggtca
    18901 acaagcgggc aatgtgcgga acgaccaggc cgacccaacc aattatgcct gctattgcta
    18961 ccgtgctggc ggtgatgagt gtggcgcaga caatcaatcc aaagcgcaga cgtgtcacgt
    19021 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    19081 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    19141 aggtcacggt ggacagcccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag
    19201 gatcggccag cgtttttatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc
    19261 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    19321 cgcccgcgac caccatcagc ccgccgcaaa atgcgtacag ctggatgtaa acaatggata
    19381 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    19441 ccggtgacac taagggatta cggaacattc cctgataggt ggttccggcc cctgccagcg
    19501 ccgcgcccag aaacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    19561 gttcctgcac aggaacattt tcttgacgaa gaatgagcgc cagcagttgc tgcatatcga
    19621 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    19681 atccgtttac ggttctcatt agctcgccac caatttttga tagtcggcgt cactgagtga
    19741 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    19801 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    19861 atccagccag ccaaagggca ggccacttaa aaataagatg cgttgttccg caaccgcttt
    19921 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    19981 aataatatcc ggctgccagc gcaggagatt ttccatggaa acctgtgtca gaccgtggcg
    20041 atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga
    20101 accctgtaag cccgtttcca gccctcgagg accgcgcgca gcataaaagc tgagattagc
    20161 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag cggctggcga gattggcttg
    20221 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    20281 ttgttccagt ttcccgttaa gcaataacca gggtatttgt gtctgttcgc taacctgccg
    20341 tgcctgggag atccaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    20401 cgccataagc ccttccagcg agagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    20461 gaactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    20521 aaaaccgacc attttttctg gcgcgacagc cagcaatagt aaatcggcgg gcgcgcctgc
    20581 gctgacgatg cgtgtaatgt gttgggcttc gggaagcgtc agtgaggccc agaatttgac
    20641 tgattgtgca aaggacggca gatgatgcag gaacaatgtc gacgcaagaa tctgtgtaaa
    20701 cctccgtctg gttagtgaca taagtaatat cctgataatt aaaaccgcta tatacttaca
    20761 catatagcga aaaagatcat gtttgatgtc aaaatttgtc taaagagaca gaatgatttg
    20821 cttgtttctt aaataacact ttgttccaca agaaaattgt cagggagaat ggggagtggg
    20881 ggaaaatata aatagaggtg tgatgattaa caagttgata aaataaaatt aaaatcttct
    20941 ctaataaaag aaaataaagg catagaccgt agaaagagtg gtatttcgtg atgaagaaag
    21001 agcaatgcca attttatgca cgtaatggca ttgctcgata tacagtaaag ttaaggcgta
    21061 ggttccgcct ctttttgttt tagcggctgt gttgttgatt cactgagtgg acgcgtcgcc
    21121 ggaacattgt cacacggttg ctctttcggg cagatcaaat ccagcatttt cagcgtcacg
    21181 ccattggtcc agccaaagcc atcctgtaat ggatattcgc caccgccgcc ccctgttccg
    21241 gtggtgctga catcatattt ttccaccagc tttttctccc ggtcataggt gtgctgaaca
    21301 ttggtcagga agtgccagct aatgtccatc gctacctctt tttgcccgta gttttgtaat
    21361 ccttctgtcg cgacccactg caacggcgcc cagccgtttg gcgcatccca ttgttgccca
    21421 cttttcaccg acgtggtgtt caggccgcct ggttgcagca gatgtgtttt cgtcgccgtc
    21481 gccattttgc tggcgcgatc tttcgctgcc gcattgacgt acagcgggaa cagggcggcc
    21541 gcggttaact gattgcgcac tttatgactt ttcaggtcgt aatcggcata ccagctttgt
    21601 tgatcgttcc acaggtattt ttcgatccct ttttgacggg catttgccag cgtttcgtac
    21661 tggtttgcca tcgcgttatc tccgatagct ttgctggcgc gggcgaggat tttttccatt
    21721 ttgaacatca agctgttcag atcgaccggt acgatgctgg tggtgcgtaa ggtgtttaac
    21781 tgctgcggat tgtccatcca gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc
    21841 aggtcgcggt aaatttcagt ggcaggtcga ttcggattgc ttttggcagt ggcaatatct
    21901 tccacccatg actctggtcg tggcgtatcg cgatcgtccc agtagcggtt gagaagggta
    21961 ccatcctgaa gttttacaac gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg
    22021 tccatccagt aagcatattc tttttgcatt tgcggcaggt attgtttcaa cgcggcgtcg
    22081 ccttcatgct gcgccagtaa ctctaccatc agggcaaaga agggcggttg cgagcggctt
    22141 aaatagtaac tgcggttgcc gttgggaata tgaccgtaag tgtctatttc atgagcaaaa
    22201 ttggccacca tatccgcgac tttatcccag tgaccgcttt cggcaagtcc taacatggtg
    22261 aagtaactgt cccagtaata tacctcgcga aaacgtccgc ctggcacgac ataaggcttt
    22321 ggcagcggta atagagaatc ccatttttcg gtgttttcgg tagaacgcgt taataccggc
    22381 caaagtccgt caatatgttc gcgcagtgat tgcccctctg gcggaacata tttctcgccc
    22441 tctttcggca gggtgaaatt gacgttaacg aaatggcgta gatcaaatcc gctctggttt
    22501 tgctgcatcc gataatcagc aaggatcatc agcggatcgc tgttcggcac ggcatcggca
    22561 aaggtttttt ggtccggaaa aagtttggcg ttttgcacat cattaaacag cggccctaat
    22621 aaaatatcag gcggttgtgg tgttaccgat gtttcttctg cctgcaccga tagcgcagcg
    22681 aaacacaaaa agatgcaggc tggaattaac gccatttttt gcgggcgaga aggtgcgggg
    22741 gatttcatca atcattctcc tttggcgaaa ccgaataaaa cggtattcaa gtatcagaaa
    22801 accttagttc aggatcgcac tagctgcatg atcgaaatca cattctgcga actgacagac
    22861 attttagaaa agggaattaa agggataaat aacggcgaaa tagagtgata aaataacatc
    22921 tgtttattcg tcagccagcg acgttgggtt cgcgattagc gaagaaaaat catcataatt
    22981 tggtgaaaaa tataacaggt agttaaaaaa catcagtgca gagtaaattg ccggatgaca
    23041 tcagaatgat gccatccgaa cggtggttta accctgacgg ttgaaacgtt gcgttttaac
    23101 gtccagcgtt agcgtttctt ctggttgtat cgcatacagt ttctcaccct gctggcaaat
    23161 ccagccaatt cccagttcac gggcgattag ggcactgtgg gatagcggag ttccggcgct
    23221 aaggcagata ccttttacaa ccgctggatc cagttgcagt actgtggagg gataaatgtt
    23281 ctccgccagt agaatagttg gcgagttaaa ctgcgggagt tcttctttcg tttgggtcag
    23341 gtggaccagg gtgcgatgca gaagatcgtc cacatcgata tagcgagcct gtagatattc
    23401 atcatccagc tgttggtatt gctggctaag ttctttaaga acttgctgcc aggcatattc
    23461 cgccgtgcaa tgttcatgct gaaggagttc gcttgccgcc gcctgcagtt ccggatcatc
    23521 taacagtgta tggtgaccag aaaagattgc ggcaatatcg tcaagcccgc tggtttctgc
    23581 tttcgctgtt aacgtcatca gatctaataa cgtgaagtca atggcctggc gtaatcgttc
    23641 ttgttcttct tccacggtca gggttgattt tgcctgtacc gtacataaaa caggttgata
    23701 ataaaaggct ttacccgaaa caggcggaac gggacgcaga gtaggtggag caacttcctc
    23761 cgtttcacca aagttatctt cagccaactg acggaaagcg atcagtgcct cttcagcttc
    23821 tggtcctttc gcaatcaggc gcagcgtatc gttatagcga acttgtagta acgcaatctg
    23881 gttaatactc tccggtgtaa cgcatttgcc gtttttctcc agcaacatat cggcattaaa
    23941 tgtcgataag gtataaacca gccgggaggc cggacgtaca tgcaggccgt tacggttttt
    24001 tatgaccacc gccagagaac gggcttcttc atcgtacgga ggacatgtgt cagagatttc
    24061 agtgtcggag gacggtaaac ccagttgttc acgtttggct tccagcgcat gcatggcgtc
    24121 aaagataact ttgtcgatat ccgcccccga ggccgcgctg accgttgctg ccagtgtacc
    24181 ttcgaccaac ggcgcagcac acaaacgtac ttttgcggcg atctcgggag ccagcaattc
    24241 cagcgcagtt tcagcactca ataatgcgct acccatatcc atcatgacca gcacatggtc
    24301 ggcatcggca acagattcga tggcctccat cactttgacg gcatcggtac caatgggatt
    24361 ttgtggatcg tcaattcccg cggcaatggc gattttacaa ttatcactca ttaacatctg
    24421 acgggctaat tcaccgacac cttctcccag tcggctgcta tgtgaaacta tgaccaggtt
    24481 taccatcacc aattccttac tcttttgcgg ctaacgccaa catttgcatc ataaacatca
    24541 ccgaggtcgc gccgggatcc tggtgaccaa tactgcgttc accgagataa ctggcgcggc
    24601 ctttgcgggc ttgcatcgta atcgtacttt gtgcagcgga ttcggcgatg ctactggcag
    24661 cgtcgagcgc cgccggaaca gagagatttt gctcgcagga ctgacgtaac gattccacca
    24721 ccggcaccca cacatcacac atggttttat cgccaggttc ggctttcccg cgactgatta
    24781 cgccgtccgc gccatcgcgg aacatctgat aaagctcttc cagcgtcagg ctttgccgtg
    24841 cctgggttgt ctgtgcggcg cggataaaga aggtaccgaa cagcggacca ctggcacctc
    24901 cgacgctgga aagtagcgtc atgccggtat tcttgagaat gaaaccgata tctttatctg
    24961 cgatagcagg gagtttttcc accactttgc caaagcctcg gttcatattt agcccgtggt
    25021 cagcgtcgcc aatctcgcga tccagtccag taagatactc gctctcggtg ctgaaaatat
    25081 cgccacaacg agtgagccag ttaacaattt gagttctgct cagtgacatt gctttctcct
    25141 tatttacccc agttaagggc cggggtgtgg accggggcgt cccagagtgc cagcgtttcg
    25201 tcatcaactt tcagtaaggt gattgagaaa ccggtcatat ccagtgaggt gcagtacgcg
    25261 ccaattaaat tacgttcgat agtcaatccc gcttgctggc aacgtgtggt caggcggtta
    25321 tagacgccgt acagctcaga aagcggagtt gcgccaagat tgttaaccag cgcaatcacc
    25381 cgatcgccag actggagcgg ttgtttggtt tgtggttctt cctgccagct gccttgttga
    25441 taatcccata aacgcaaagt gcgatggtat gagccatttt ccagcagggt gtcgaacatt
    25501 tcatcgacgg tttgatcaag ggaagagaag gggcggcggt caatacctgg ctcaccatga
    25561 atgccgacgc caaactccat ctcattatcc gccagggtaa aagaaggttt gcccgcggca
    25621 ggaacggtac aggcaccgag agcgatacct attgagtggc cttgattatt caacttacgc
    25681 cccagttccg cacaggcgtc cagtgagtcg ccacgctccg ccgctgcacc tacgagtttt
    25741 tcaattaata cggtgttggc aacgccgcgt cgcccggcgg tataaagact gtcttttact
    25801 gcaacgtcgt catcaatgac cacagtggtc acttttacgc cgctatcgtg cagtaactcg
    25861 gttgctgttt cgaagttaag aatatcgccg gtgtaatttt tgataatcaa cagtacacct
    25921 tcgccgccat caatttgcat ggcgcattca aagattttat cgggcgtcgg tgaggtgaaa
    25981 atttcgcccg gacaggcccc cgaaagcatc ccctgaccga tataaccaca atgcatcggc
    26041 tcgtgtccgc tgccgccacc cgacagcagg gcgacttttc ctgcaacggg ggcatcagct
    26101 cgggtgacat acaccggatc ctgatgcagt gtcagcgatg gatgcgcttt cgctagtcct
    26161 gccagttgtt cgtccagtac gtcttgcaca tcattgatca attttttcat tattttgctc
    26221 cagcaattac ggtagggcat ggatgatgtt caacgacacg gcgaccactg actgccgatg
    26281 aatccattgt gcatcaggca agggaaaaga aaattagccc aaattatgtt tcatagtgaa
    26341 acatatgctt taatgagtgt tccatattga aacttttacg tgtattaata cttaaaattg
    26401 cgagccggaa caccttttgt cataagggat gcgggatatg agtggcgctt ttaacaacga
    26461 tggtcggggc atatctccct taattgcaac ctcctgggag agatgcaata agctgatgaa
    26521 acgggagaca tggaacgtac cacaccaggc ccagggcgtg acatttgctt ctatttatcg
    26581 gcgtaagaaa gcgatgctga cgctcgggca ggctgcgctg gaagatgcct gggaatatat
    26641 ggcaccgcga gagtgtgcgt tgcttatcct cgatgaaacc gcctgcattc tcagccgtaa
    26701 tggcgatccg caaaccttgc agcagctaag tgtactggga ttcaatgacg gcacgtattg
    26761 cgccgaggga attattggta cttgtgcgct atcgttagcg gctatctctg gtcaggccgt
    26821 gaaaacgatg gccgatcaac atttcaaaca ggcactctgg aactgggcct tttgcgcaac
    26881 gccgttgttt gacagcaagg gccgattgac gggaacaata gcgctggcgt gtccggttga
    26941 gcaaactacc gcagctgatt tgccgttgac gttggcaatc gcacgcgagg tcggaaattt
    27001 actgctgacg gacagtttgc tcgctgaaac taaccgtcat ttaaatcaac ttaatgccct
    27061 gttagaaagt atggatgatg gcgtgattag ctgggacgag cagggtaatt tgcaatttat
    27121 caacgcccag gcggcgcgag tcttgcgcct tgacgcgacg gcaagtcagg gaagggcaat
    27181 cactgaactc ttaacgttac ccgccgtatt gcaacaagca ataaaacagg cacatccgct
    27241 caaacacgta gaagcaacct ttgaaagtca gcatcagttt attgatgcgg tgataaccct
    27301 taaaccgata atagaaacgc agggaaccag ctttatttta ttgctccatc ctgtggaaca
    27361 gatgcggcag ttaatgacca gtcaattagg aaaagtcagc cataccttcg ctcatatgcc
    27421 acaggacgat ccgcaaaccc gccgcttgat tcattttggt cgccaggcgg cgcgcagtag
    27481 ctttcctgtc ctgctttgtg gagaagaggg cgtgggcaag gcactgctaa gtcaggcaat
    27541 tcataatgaa agcgagcgtg cagcggggcc ttatatcgcc gtcaattgtg agttatatgg
    27601 tgatgcggcg ctggcggaag aatttattgg tggcgatcgc acggataatg aaaatggccg
    27661 tctgagtcgg ctggaactgg cgcacggcgg cacgctgttt cttgaaaaga ttgaatatct
    27721 ggcggtggag ttacagtctg ctttgcttca ggttatcaag cagggtgtta tcacgcgact
    27781 ggatgcgcgg cgtttaatac ccattgatgt caaagtgatt gccacaacga ccgcggacct
    27841 cgcaatgctg gtggaacaaa atcgttttag tcgccagctg tattacgcgc tgcatgcatt
    27901 tgaaattacc atcccgccac tgcgtatgcg gcgtggcagc attccggcgc tggtgaataa
    27961 caaattacgc agccttgaaa aacgcttctc tacgcggctg aaaattgatg acgatgccct
    28021 cgctcgcctg gtttcctgtg catggcctgg caacgatttt gaactttaca gcgtcatcga
    28081 gaatcttgct ctgagtagtg ataatgggcg cattcgcgtc agtgatttgc cggaacatct
    28141 gtttaccgag caggcgacag atgatgtcag cgccacccgc ctttccacca gtctgtcatt
    28201 tgcggaagtt gaaaaagagg caattattaa cgcagcccaa gtcacaggcg gtcgcattca
    28261 ggaaatgtcg gctttacttg gaatcggccg cactacgctg tggcggaaaa tgaagcaaca
    28321 tggcattgat gcagggcagt ttaagcgccg tggatagcgg gagagtatgt actttcccgc
    28381 tatgaattac taattaatag cgaccatgac tgccgtgatt tggtccacca tttgaaggtg
    28441 gcattggctt accggagggc gcaggaccac gagggccatc gctgtggcgg tgatgatggt
    28501 ggggaacaca agcactcaac aggcttgcaa agacaattgc taacaggctg aaggtaaatg
    28561 tttttttcat gacaaactct tgaacggtta atgcactgtg attgttatca cataaaagct
    28621 gcattgttaa tattgtcagg agtctgatta tgagttttgg taactcagaa ttgaaagaag
    28681 acgcgtagca ggcgtaaaaa agccattacg tgcaactcac ataatggctc gttattaacc
    28741 gccagagtag tattagcgga attagacgtt gaattgtaat ttttgctgta tctgattgaa
    28801 atttatacta catttaatga tatatttttt ttgtactcat gttcgtactc attttggagt
    28861 tagctcgcat tcgtaatgcg aagggcgtag gttctactcc tctcagcaag gatactgccg
    28921 gcagaatcct tcatgaggct ggaatgctca aaaagtccac cagcgtggag ggatggctga
    28981 tacgtatgcc acggctgaga cacctcaagc atgcacgtcc ctgggcttat acgctgttgt
    29041 ttactccggt atctgaaacg gaaaccggag aagaataaaa aaacacgtaa atatgaaaac
    29101 tgttagccca catagcccaa caaaaatatt tgatagttaa ccttctgtta ctaaagaaaa
    29161 caggaaagta aaagtgggct aaagcttatg cgccctcgat gttgggctag ccccaaaaac
    29221 ggtaaattta gcttaagtgc ataattggtt agctcaaaag cattattttt catttaaata
    29281 aattagttaa ttggtcttgt ttagatgatt caactgggct gactactttc tttgtatata
    29341 ctccggataa attttcccag ctaacttgcc taatcatcac tctgatgcca gaaatgaaca
    29401 gaacgcaaac catctataac ttattgagga ttttgaaaaa aattgattgg gggcttgagt
    29461 tatatgatga ctatgctaat ttaatacggc acatgcaggt agatttgttg gttgtggtat
    29521 cgcaatcagt gttaacaagg tcgggagtat tcgccctctg actgccgtca agtcatcttg
    29581 gcgtcaccgt taaatgcgta agagtacctg catgtgcatt aacataatca ataatggaat
    29641 ttactgttat gtttaaacct acctatctgg caaggctgca ggcttgttgt aacaaatttg
    29701 aactggctga tttgcttcag attaaagtta catttctgac taatgttttg tatagaataa
    29761 ggccagaaaa tcaatacaaa aaatttacta taaagaaaaa gtctggagga gagcgggaga
    29821 tctttgctcc tgatgaaaaa ctgaaagata ttcaacaacg actttctgaa cttctatata
    29881 tatgccagga agaaatttgg gcaaaaaata atattaaaca aaatgtatca catggttttg
    29941 agaagaataa aactataatt acaaatgctg agaggcatcg agataaaaat attgtattta
    30001 atattgatat tgagaatttc ttcccatcct ttaattttgg tcgcgtgcga ggatatttta
    30061 ttgcaaacca aaatttcaag ttacatccaa atgttgcaac cattattgcg cagatagcct
    30121 gcctggatgg atcgcttccg caaggaagcc cttgttctcc agtaataact aatcttattt
    30181 gtaggatttt agatttcaga ttatcaaagc tagcagtcac atatggttgt agttacagcc
    30241 gctatgcaga tgacattacg ttttcaacaa acaaaaaaaa catccctgat gcattagttt
    30301 ctaatgagaa agaaaacgaa ccaggtaaga tattggtaga agaaattcat cgtgcaggct
    30361 tcactttaaa ccataataaa aacagagtgt ctaggtgtac atcaagacag caagttacag
    30421 gtttaactgt aaataaaaaa ataaatgtaa gcagagagta tataaagaat acaagagcga
    30481 tggcgcattc tttatacttt gaaggttcgt atacactcat tgagaaagat ggaaaacata
    30541 gaaagggcac ccttagtgaa ttagaagggc gatttgcatt tatcgatatg cttgataaat
    30601 ataataatgt ggaagcaaag aaaaatgcgc gtcctgagag atatgtggtt aaaggatttg
    30661 ggttggattt taagcagaga cttaactcca gagagaaagc atacagcaaa ttcctatact
    30721 ataaaaattt ctatggaaat gagcaaataa caatcttaac agaagggaaa actgacccgg
    30781 tttatcttaa gtgtgcaatt gattctttgt ttttggatta ccctcagtta gttagagagg
    30841 aaaaaaacac aaagaataga gtgttaaaag ttaatttatt taaaaccaat gacaagaaaa
    30901 aatattttct cgatttgtct ggtggagctg cagactattc gaggtttttc agacgacatg
    30961 gtttactttg taaagcgtat gaaaaacagc ctcctaaaaa tccagtgata attttattag
    31021 ataatgacac agggccatct gacttcataa atcaaataat aaaggattat tcgcatctac
    31081 caaaaaaagc ggaggatgtt agaaaagggg cgttttatca cttagagagt aatttatatg
    31141 ttctttttac tccgttatta ccaggggata actattcttc actagaggat ttttttgaac
    31201 caaaagtttt gcaaatgaag tataatggaa aaagcttcga taaaagcaat aatcatgaca
    31261 gttctactac atttggaaaa gatagatttg ctacttatat agtaagggaa aatagaaaaa
    31321 ctatcgattt ttcattattc aaacccatac ttgattcaat tattgaaatc aaaaaacatt
    31381 ttatcaatct acacccatca aagtgatggt tatgaaaaga gataaaaatg ctgatgtcaa
    31441 aagaggctta tgctcggcac agtggagtga gctgccaaac tgtcgatgac tgggtagccg
    31501 gtggggcgga agtagttatg tcccgtagca aggttaagat ttgctcttgt gtgtggggaa
    31561 ccttagtcaa ttactttcct ggcgcactgt gttagatttt gtaaaatttt aaaagactaa
    31621 agatttaata tcacttctcc atggaggttg tggtggtaga gaagatgaaa caaaattcaa
    31681 gttcgacgct tggcgcgctt tctgctccta caacgtggcc gggtgcgaac gctcggctgc
    31741 ttgggttagg tgtagggctt ccaatagccc ccttggatcg tctggcacag tttagcgccg
    31801 ccgaatttga aaggtttaca ctcgaatggg cctctgaata cttagctggt cagcaatatg
    31861 tcaacgaagt acaacaacgt ggcggggcag gagataaagg cagagatatt gttgtatggc
    31921 ttgatccatc tgatgctgtt cctcgaagat ggcacttata tcaatgcaaa cattatgatg
    31981 cgaatttagg ccttcctaag gcaggcatcg aagttgcaaa agttatctat tatactttca
    32041 tcaaggacta cacagtacct acacaatacc attttgttac acataaaggg gtaacatcgc
    32101 cattccaaga cctcctagat gatccaacta aactcaaaga gaaaatgcta tccgagtggg
    32161 caactttttc aaagcaaatt acatcgaaac atagtgttga cctgacgcct gaacttgaaa
    32221 aatatattaa agagtttgat tttagtattt ttcatgcaaa acagcctatt gagatacttg
    32281 ccgaacacag taaaacctct tttcatctta tggtctttgg cgcaccatta attgagcggg
    32341 atccaccaac taggccccca tcatctgtgg ctcccattga aactgtatat attgaacaac
    32401 tcttttcagt tatcagcgct gatatcaaaa ccaacgtgcg agatttagtt gattttcaat
    32461 caagtatcag ccatgtaaaa ctctttgaac ggtcccgaat aactttttat tgctcggaag
    32521 ggctaaagga actcgctagg gaccaaatgg cgaatcaaga attctttaat tctttacttg
    32581 tagagtttga tgatggtctg tatcactaca cctctgacct gacagggact ccactccttc
    32641 ggttaaaaaa taccgtcaag gccgctcaga ccctgcaact tggtgcgcac ccacttgcca
    32701 ttcacgtaac gaataaggat cgtgaaggta tatgccacca actagccaat acaaacttga
    32761 ttaattggtg caatccatga gcgaaatgca agtatttgaa ggaaactttg agcagagacc
    32821 ttttaacact ccgctggagt atggatttcg tgctctgttc atattggatg cagcggacaa
    32881 tgtagcaatg gacctacaaa ggatagtttc gtatgactat cttcttgtgc ataccagtga
    32941 cgtacctagg gggcctaaaa gccttcatcc agcagttcct caccggggag cagagcttct
    33001 tgttaagcga agcgcaattc aggcagggct gcatttaatg ctatcaagag aattgattaa
    33061 ggttgtcttt gctgccgaag gtattttgta tcaggctacc aatttaacag gaaggtttgt
    33121 gcggcttttg atgtcacagt attcaaaaga gctcgcagaa cgtgcctctt gggtcacaaa
    33181 gcaattctat gaatatacgg atgaagaact tgcttcatat atatctcaga atgtggggca
    33241 atggggttct gaattcgacc gattgaccgc aattgatcta ctggatttat aacatgcttc
    33301 gtataagaaa aatactattg cgcggaaaca gcgttcaaga cgcttatgtt gatttctata
    33361 agggtgcgaa tattctagca ggggaatctg atactgggaa aagttacctt gtgagttgtc
    33421 ttgactacat tcttggtgcc gaaaaactca aaaaaaagct taaagaggcc accgattaca
    33481 cccatcttta tgttgaattc gaaaatgatg aagggggggt gttaacgctc aagcgcggtc
    33541 ttgagggggg gaaattagag gcacatgatg tcgctattca agacatccat ggagaaggta
    33601 aaataattgc tcctgtgcgt aaaggaacga gtaaggggcc agatgtgacg tccatacttt
    33661 tcccttttgc ggggatcaaa gaggcgaagc ttcgcaagaa tgctcgtggc gaaacgcaac
    33721 gtttctcgat tcgaacgctc gctcctatat ttttggtaga tgaagtatcc attatcgacg
    33781 aatactcgcc agtaacggga cgttccggtt atgacgacac tgctcgaaaa cgcatgtttt
    33841 catacatcct taccgggcac gatgatggtg gtgttaccgt agaggaaaag cctgaaattg
    33901 taaaagcgag gttgatggcg aagcttgaat tcatccagga tttgattcga ccacttgatg
    33961 aacgtttctc aatttgttcg cccaagtttc cattaacgag ctcagcagat gatttatcag
    34021 atcaattgat tgcacaagca attgatgaag ttgagagggc tgcagccgct atttctgact
    34081 tgctcgaggg aataaaaatg gagacagcgt taacgttaaa aatagaatct cagttaatgg
    34141 gagtatctga aattcaatcg agatatagcc tattagaaga acgttaccat tccgacctga
    34201 agcggttgga ttttatttcc gaaggttctc actatttcac ttcacttcag gaagttcctt
    34261 gttccctttg tggacaaaat ttactacatc cacacagtga gaacgctaaa aaattaatga
    34321 acagtaatga agtccgacgt tcttctttag ctgaagctgc aaaaattcat ggataccttg
    34381 caggcctgca aaaagcgatg agtgatcttg accgaagaaa agaagctctg aatatagaca
    34441 gatataagtc aaaagaaagc ttggatggaa tgaagaacca gatcaaatat acttttgagc
    34501 ctcttcttac actttccaaa gaaagcctag atttagctat tgagcagcga gctgaaagag
    34561 actctgagtt gaatgatcga cagagatggt ttgagttact aaaacatcaa aaggatatag
    34621 aaatactgct ggaaaagagc agtcaaccac ggctcgaatg ggagggatta tctacccgta
    34681 cgctggccga actttgtaga gaaattgaaa ctgttttaaa agactggaaa tggggtgccg
    34741 agcctgacgt ttcatttaat gaaaaagagt atgacatcat tgttgatgga caacctcggc
    34801 agtctcacgg taaaggtgtt cgtgccattc tttattcagc tttcatcatt ggattactaa
    34861 aatattgtat taaaaaagga tacccgcatc ctgggctagt cgtgattgat tcaccgctga
    34921 caagttataa gaagaaagcc gctgctacgg tatcgggctt cgatgaaccc atatcggctg
    34981 gtattgagga aggcttctgg cattcactga cccaattgcc tgatacctta caaatcatag
    35041 ttatagagaa taaagaaccg ccggtatcaa tcatgaataa agttcattac gaatggtttg
    35101 cgggtgaaca tgcaatggat ggagagcgca gagcccttat cccgctgccc tcaaaaaata
    35161 gtgctatttt aatgaagtaa cttaggatgg gtagccatca aatcgcagtt atacgattgg
    35221 tggggtttgt aaaagcggta gtcttgatta ccactgcatt cattctacaa gcacatactt
    35281 ccctaggttg ctgtagttat ccgattacac gccttttctg aaagcagcct cagggagccg
    35341 gataaaaccc gttaaaagtg gcttgcctat actgctgcgc ggcgctgacg ctttagaggc
    35401 cccaggattt aggcaaatca atgtagttag ctcagtagca ggtcatatct gggctataaa
    35461 tggcattacg tgacactgaa agagcttccc agtggcatcg tatttggtga gccccaacct
    35521 acaagacggg cggggatagc cgtgattggc acccacagca aggcgataaa gatgttcaac
    35581 ggagcggctc agcaggtatg gggatggtag gatttagtct tcatatgcct gtggaattaa
    35641 tcaaccctca gagcccgaca cacccggcat tgcttacggt ataagactga ttgagaaggt
    35701 gtttgacgtt tttgtatccc agtctgtcgg taaagtaaac acagtgatca gtaactggta
    35761 gtactggaag tgattgcggt cgtaatgctg attttgcacg ctacgacagg tatattctgt
    35821 ttggtatctg tagaggttgt gatggcgctg accttgcaca tgccaggtac gttagactta
    35881 ataataaagg gagtattagc aggagggatt ttttcggact cagttgtatt gtgtgtaacg
    35941 agccaactaa atgtgataat agctataatg gtaaatatga tgatcttttt catagggttt
    36001 ccttttggaa taaaacatga attatttcac ccaaa
//
LOCUS       DAOFSV010000037        35780 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.037, whole genome shotgun sequence.
ACCESSION   DAOFSV010000037 DAOFSV010000000
VERSION     DAOFSV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.037"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            334..1719
                     /gene="dcuC"
                     /locus_tag="QBH27_001927"
     CDS             334..1719
                     /gene="dcuC"
                     /locus_tag="QBH27_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDQ7014240.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1760..2440)
                     /gene="dpiA"
                     /locus_tag="QBH27_001928"
                     /gene_synonym="citB"
     CDS             complement(1760..2440)
                     /gene="dpiA"
                     /locus_tag="QBH27_001928"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDQ7014241.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2409..4067)
                     /gene="dpiB"
                     /locus_tag="QBH27_001929"
     CDS             complement(2409..4067)
                     /gene="dpiB"
                     /locus_tag="QBH27_001929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDQ7014242.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELYLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4447..5505
                     /gene="citC"
                     /locus_tag="QBH27_001930"
     CDS             4447..5505
                     /gene="citC"
                     /locus_tag="QBH27_001930"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDQ7014243.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5520..5816
                     /gene="citD"
                     /locus_tag="QBH27_001931"
     CDS             5520..5816
                     /gene="citD"
                     /locus_tag="QBH27_001931"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDQ7014244.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5813..6721
                     /gene="citE"
                     /locus_tag="QBH27_001932"
     CDS             5813..6721
                     /gene="citE"
                     /locus_tag="QBH27_001932"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDQ7014245.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6732..8264
                     /gene="citF"
                     /locus_tag="QBH27_001933"
     CDS             6732..8264
                     /gene="citF"
                     /locus_tag="QBH27_001933"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDQ7014246.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCADLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSQAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8268..8819
                     /gene="citX"
                     /locus_tag="QBH27_001934"
     CDS             8268..8819
                     /gene="citX"
                     /locus_tag="QBH27_001934"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDQ7014247.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8794..9672
                     /gene="citG"
                     /locus_tag="QBH27_001935"
     CDS             8794..9672
                     /gene="citG"
                     /locus_tag="QBH27_001935"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDQ7014248.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9723..11186
                     /gene="citT"
                     /locus_tag="QBH27_001936"
     CDS             9723..11186
                     /gene="citT"
                     /locus_tag="QBH27_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDQ7014249.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11300..12106
                     /gene="rna"
                     /locus_tag="QBH27_001937"
     CDS             11300..12106
                     /gene="rna"
                     /locus_tag="QBH27_001937"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDQ7014250.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12336..12746
                     /gene="rnk"
                     /locus_tag="QBH27_001938"
     CDS             12336..12746
                     /gene="rnk"
                     /locus_tag="QBH27_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDQ7014251.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            13102..13530
                     /gene="uspG"
                     /locus_tag="QBH27_001939"
     CDS             13102..13530
                     /gene="uspG"
                     /locus_tag="QBH27_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDQ7014252.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(13651..15216)
                     /gene="ahpF"
                     /locus_tag="QBH27_001940"
     CDS             complement(13651..15216)
                     /gene="ahpF"
                     /locus_tag="QBH27_001940"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDQ7014253.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(15387..15950)
                     /gene="ahpC"
                     /locus_tag="QBH27_001941"
     CDS             complement(15387..15950)
                     /gene="ahpC"
                     /locus_tag="QBH27_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDQ7014254.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            16322..17068
                     /gene="dsbG"
                     /locus_tag="QBH27_001942"
     CDS             16322..17068
                     /gene="dsbG"
                     /locus_tag="QBH27_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDQ7014255.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
     gene            17273..18175
                     /locus_tag="QBH27_001943"
     CDS             17273..18175
                     /locus_tag="QBH27_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7014256.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            18322..19542
                     /locus_tag="QBH27_001944"
     CDS             18322..19542
                     /locus_tag="QBH27_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ7014257.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKSITVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRKEWGILCNND"
     gene            19515..20144
                     /locus_tag="QBH27_001945"
     CDS             19515..20144
                     /locus_tag="QBH27_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDQ7014258.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(20145..21305)
                     /gene="ybdL"
                     /locus_tag="QBH27_001946"
     CDS             complement(20145..21305)
                     /gene="ybdL"
                     /locus_tag="QBH27_001946"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDQ7014259.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTKMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            21414..22502
                     /gene="hcxA"
                     /locus_tag="QBH27_001947"
     CDS             21414..22502
                     /gene="hcxA"
                     /locus_tag="QBH27_001947"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDQ7014260.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(22512..22709)
                     /locus_tag="QBH27_001948"
     CDS             complement(22512..22709)
                     /locus_tag="QBH27_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDQ7014261.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(22822..24927)
                     /gene="cstA"
                     /locus_tag="QBH27_001949"
     CDS             complement(22822..24927)
                     /gene="cstA"
                     /locus_tag="QBH27_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDQ7014262.1"
                     /translation="MNKSGKYLVWTVLAVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGEVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(25108..25521)
                     /gene="entH"
                     /locus_tag="QBH27_001950"
     CDS             complement(25108..25521)
                     /gene="entH"
                     /locus_tag="QBH27_001950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDQ7014263.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(25524..26270)
                     /gene="entA"
                     /locus_tag="QBH27_001951"
     CDS             complement(25524..26270)
                     /gene="entA"
                     /locus_tag="QBH27_001951"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDQ7014264.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFSQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIACPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(26270..27127)
                     /gene="entB"
                     /locus_tag="QBH27_001952"
     CDS             complement(26270..27127)
                     /gene="entB"
                     /locus_tag="QBH27_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDQ7014265.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(27141..28751)
                     /gene="entE"
                     /locus_tag="QBH27_001953"
     CDS             complement(27141..28751)
                     /gene="entE"
                     /locus_tag="QBH27_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDQ7014266.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(28761..29936)
                     /gene="entC"
                     /locus_tag="QBH27_001954"
     CDS             complement(28761..29936)
                     /gene="entC"
                     /locus_tag="QBH27_001954"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDQ7014267.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            30125..31081
                     /gene="fepB"
                     /locus_tag="QBH27_001955"
     CDS             30125..31081
                     /gene="fepB"
                     /locus_tag="QBH27_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDQ7014268.1"
                     /translation="MRLASLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIALFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(31085..32335)
                     /gene="entS"
                     /locus_tag="QBH27_001956"
     CDS             complement(31085..32335)
                     /gene="entS"
                     /locus_tag="QBH27_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDQ7014269.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLSVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            32446..33450
                     /gene="fepD"
                     /locus_tag="QBH27_001957"
     CDS             32446..33450
                     /gene="fepD"
                     /locus_tag="QBH27_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDQ7014270.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            33447..34439
                     /gene="fepG"
                     /locus_tag="QBH27_001958"
     CDS             33447..34439
                     /gene="fepG"
                     /locus_tag="QBH27_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDQ7014271.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            34436..35251
                     /gene="fepC"
                     /locus_tag="QBH27_001959"
     CDS             34436..35251
                     /gene="fepC"
                     /locus_tag="QBH27_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ7014272.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(35248..>35780)
                     /gene="fepE"
                     /locus_tag="QBH27_001960"
     CDS             complement(35248..>35780)
                     /gene="fepE"
                     /locus_tag="QBH27_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDQ7014273.1"
                     /translation="ESIENVRNKLEIKTQFEKEKLAQDRIKTKNQLDANIQRLNYSLD
                     IANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIEKAVTDVAELNGELRNRQY
                     LVEQLTKTNVNDVNFTPFKYQLRPSLPVKKDGQGKAIIVILSALVGGMVACGGVLLRH
                     AMASRKQDAMMADHLV"
BASE COUNT         8613 a         9675 c         8916 g         8576 t
ORIGIN      
        1 aagatataaa aaatatctta acgaaaaata tattatgtat cgtcaaccca tgctcatgga
       61 agattatttt ttgagcacac aaccgcagct aacaccttac aattaacaaa tttgcatcaa
      121 tccgccatca atttacacac attattatgt gatatttgcc aaccgctaaa tatgcgtttt
      181 gttatctacg tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa
      241 tcagcattca ccgccaattt attgggcata ttttttcctt aacttttagg aattttttat
      301 tatttacttt ggggcctgga gacaggaaaa attatgctga cattcattga actccttatt
      361 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc tgccactggc
      421 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      481 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      541 atattgctaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt
      601 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      661 ttgcagtata ttaactcccc ttaccttctg atgattgccg cctattttgt tgcctgtctg
      721 atgtcactgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctgttt
      781 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      841 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcagcgca ggcttccgaa
      901 atgtcgctga ttgacttcgc cttcaaaaca acgctgccta tctcaattgc tgcaattatc
      961 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
     1021 catgaaatgt tagatgtcag tgaaatcacc accactgccc ctgcgtttta tgccattttg
     1081 ccgttcacgc cgatcatcgg agtgctgatt tttgacggca aatggggtcc gcaattacac
     1141 atcatcacta ttctggtgat ttgtatgcta attgcctcca ttctggagtt catccgcagc
     1201 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcggtat ggcagatgca
     1261 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
     1321 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1381 ctgatgctgg tattggtgat cctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1441 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcctc cggcattaac
     1501 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1561 gtttctggcg tagtcgttgc ggttgccggg atggcgaaga tctcgccgtt tgaagtcgta
     1621 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1681 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1741 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgcggtctg ccaactttgc
     1801 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1861 cagtggtgcg actgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1921 gctctttgaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt
     1981 cgtctttggg ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg
     2041 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     2101 gacgttcata ggcaattggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     2161 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     2221 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     2281 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccaggttt cccgccagta
     2341 atatctggct gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2401 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2461 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2521 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2581 cgcgtactga caccctgctc aaatattttg tctcgtagag actctggaac gccgcagccc
     2641 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggtataa ttcaacaatc
     2701 ttgtttcctt catcgctacg caggcttgct tcgaaggcgt tatcaagtaa attgccaaca
     2761 atggctgcaa actcggtgct gtccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2821 acgatgacca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2881 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2941 gactcaccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     3001 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     3061 ttaatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     3121 atggccccca gcaaatcatc gccagaacga atagcttccc gattggcgat aacgctcaga
     3181 ccgttaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     3241 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg ggagctcagc
     3301 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt acccgtgcgg atccaccgca
     3361 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3421 tgctttggct ccatgcccat catctgccga cggatatgcg cggccaggaa ccacgatagc
     3481 aacatcaaaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3541 cgccagctat cgattttact caccagatag ccaatcgaca ccacaccgat gacttttcca
     3601 tcgtcatcaa agattggcgt tttggcacgc atcgccatgc caattgaccc tttcccggtg
     3661 atgaagtagc tctccccttt ctccagcgcg cccggtttgg tgaactgcat aggataacca
     3721 attttctccg gattaggatg gtaaagacga atcgagtgcc ggtcgccaat caccacataa
     3781 tcgaaatcag tatctctttg taatttgtca gcgatggtcg ccagcctctt gtagtcacgc
     3841 gttttcactg cgctgatgat actgtcatta gaagcaataa tttttgcctg attcattgcc
     3901 atgtcgcgca catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3961 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     4021 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     4081 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaataaaaat acatagatat
     4141 taaatatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa acctgcgggt
     4201 tatttcgtaa ttacattagt taaaaaactt aaaaccatta aataaataaa aaaccactaa
     4261 ctctatgtga aataaatcaa aatttcacgc cgaaatactc cttaggatgt atagcgaaag
     4321 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcaa
     4381 tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4441 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4501 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
     4561 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt
     4621 attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct gacattagcc
     4681 actgaactga taaacctcgc ctatgagcgg cacagcacgc atctgtttat ttataccaaa
     4741 accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag cgtacccggc
     4801 gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac gctatgccga atcgctgaaa
     4861 aaatttcgtc atccagggaa caaaattggc tgcattgtga tgaacgccaa tccctttacg
     4921 aatggtcacc gttatctgat tcaacaggct gcggcacaat gcgactggtt gcatctgttt
     4981 ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggctggatct ggtgttaaaa
     5041 ggcaccgccg atattccacg cctgactgtg catcgcggct ccgaatacat catctcccgc
     5101 gcaacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg ctacaccgaa
     5161 attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt
     5221 gtcggtactg aacccttttg tcgcgttacc gcccagtaca accaggatat gcgctactgg
     5281 ctggaaacgc cgactatctc cgcaccgccc atcgaactag ttgaaattga gcggctgcgt
     5341 taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcaaa aaacgatctc
     5401 acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa cctgcttgag
     5461 cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac aggtgaaaaa
     5521 tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg atgatacgca
     5581 tcgccccact cgatacgcag gatatcgacc tgcaaatcaa cagcagcgtt gagaaacagt
     5641 ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac gtgcgcggcg
     5701 tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca cgactggaag
     5761 ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc caatgatttc
     5821 cgcttcgctg caacaacgta aaactcgcac ccgccgcagt atgttgtttg tgcctggtgc
     5881 taatgccgcg atggtcagca actccttcat ctacccggct gatgccctga tgtttgacct
     5941 cgaagactcc gtagcattgc gtgaaaaaga caccgcccgc cgcatggttt accacgcgct
     6001 gcaacatccg ctttatcgcg atattgaaac cattgtgcgt gtaaacgcgc tggattccga
     6061 atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc
     6121 gaaaaccgat accgctcagg atgttctgga tatcgaaaaa gagatcctgc gtatcgaaaa
     6181 agcctgtggt cgtgaacccg gcagtaccgg cctgctggcg gcgattgaat ctccgctggg
     6241 cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg
     6301 cgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa ctgaactgct
     6361 gttcgctcgc tgctccattt tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac
     6421 cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc acatcaaaca
     6481 gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgc tgcacaacct
     6541 ctacgcaccg acccagaaag aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc
     6601 cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg acggtccggt
     6661 tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt tccggcatcc gcgaagaata
     6721 aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg gtagcggcct
     6781 ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcgccaaag caaacctacc
     6841 aggctgaaaa agcgcgcgat cgcaaactgt gcgccgatct ggaagaagcg attcgtcgct
     6901 ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc ggtgacctga
     6961 ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg actctggcct
     7021 ccagctccct gagtgattgc catgcgccgc tggtagaaca cattcgccag ggcgtggtta
     7081 cccgcattta tacctctggc ctgcgcggtc cactggcgga agaaatctcc cgtggtctgc
     7141 tggcggaacc ggtgcaaatc cactctcacg gcggacgtgt gcatctggta cagagcggcg
     7201 aactgaatat cgatgtggct ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca
     7261 acggctactc cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt gatgccgaca
     7321 acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat aatccggcaa
     7381 gcattgagca agatcaggtt gatctgatcg tcaaagttga tcgcgttggc gatgctgcaa
     7441 aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg cgaactgctt attgcccgta
     7501 gcgctgcgga tgtgattgtt aactctggct acttcaaaga aggtttctcc atgcagaccg
     7561 gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg
     7621 atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac gatggttgac ctgcacgaaa
     7681 aaggtctgat ccgcaaactg ctggatgtgc agagctttga cagccaagct gcgcaatcgc
     7741 tggcccgtaa ccccaatcac atcgaaatca gcgccaacca atacgctaac tgggggtcga
     7801 aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag tgcgctggaa attgacaccc
     7861 agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct tccggtggtc
     7921 actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc
     7981 gtattccgac gctggtggat aacgtgctga cctgcatcac cccgggctcc agtgtcgata
     8041 ttctggtcac agaccacggc atcgctgtta acccggcacg tccggagctg gcagaacgtc
     8101 tgcaggaagc ggggatcaaa gtggtttcta ttgagtggct gcgtgaacgt gcgcgtctgc
     8161 tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc
     8221 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
     8281 ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa
     8341 ggcaagcacg gcaacacgcc tggctcaagc gccatcctgt tccactggtc tcctttaccg
     8401 tggttgcgcc agggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
     8461 tgacagcctt gcgtgcctta gccgcaaaac agggctggca aattcaggag caggctgcac
     8521 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgacctca
     8581 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
     8641 tcctgacgcc cgaaggcgac attctctccc gccgcgatta ttcactgccg cctcgccgct
     8701 gcctgttgtg cgagcaaagc gcagctgttt gcgcgcgtgg aaaaacccat caactgaccg
     8761 atttactcaa ccgcatggag gcgctgctaa acgatgtcga tgcctgcaac gtcaactaaa
     8821 accacaaagc ttgcgacgtc attaattgat gagtacgccc tgctgggctg gcgcgccatg
     8881 ctgactgaag tcaacctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     8941 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     9001 ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactcaac
     9061 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     9121 aacacgcata aaggaagcat tttttcttta gggctactgt gtgcggcaat tggccgtttg
     9181 cttcagctca accagccggt aacgccaaca accgtttgtt ctacggcggc aagtttctgc
     9241 cgtggcctga ccgatcgcga actgcgtacc aataattcac gactgacggc aggtcaacgg
     9301 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcagg ttatccactg
     9361 gtaatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
     9421 ctggcactgc tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca
     9481 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac actattaaac
     9541 aatgggggca tccgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
     9601 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgattcttac ctggttttta
     9661 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
     9721 gtatgtcttt agcaaaagat aatatatgga aactattggc gcccctggtg gtgatgggtg
     9781 tcatgtttct tatccctgta cctgacggta tgccgccgca ggcgtggcat tactttgcag
     9841 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaacg gcgatcagtt
     9901 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
     9961 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggtctg gctggttttt
    10021 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
    10081 ccgggttagg ccgtcgtatt gcccttttcc tggtgaaatt catggggaaa cgcacgctga
    10141 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
    10201 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ctgtttaaat
    10261 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
    10321 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc
    10381 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
    10441 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
    10501 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca
    10561 tgggtgcgct gacgcgcaga gagtggacgc tgattggcct tgtattgctc agcttaggtt
    10621 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt aggtctgctg gcagtttcgc
    10681 tgatgctggc gctgcacgtt gtgccgtgga aagacattac tcgctataac agcgcatgga
    10741 acacgctggt caacctggca acgctggttg ttatggcgaa tggcctgacc cgttctggct
    10801 ttatcgactg gttcgccaac acgatgagta cacacctgga aggcttctca ccaaacgcaa
    10861 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
    10921 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaagggatt ccgggcgtac
    10981 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
    11041 cttatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
    11101 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgctgctg ttggttggct
    11161 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
    11221 cgccaggtaa gcccacctgg cgggcttttt tatgatttaa tagataatat tcagtgtatg
    11281 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
    11341 tctgcttccc ttctcttctg ccaacgccgt ggcgttgcag gcaaaacagt atggcgattt
    11401 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
    11461 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
    11521 tttgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
    11581 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc tgccagaagc
    11641 acgcgccagc cggatgtgtt catcgccgga aaccgggtta tcgctggaaa cggcagctaa
    11701 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
    11761 caaacacggt gcctgctttg gttttgatcc agacgcatac ttcggcacga tggtacgcct
    11821 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataact acggtaaaac
    11881 agtgagccgc cgtgactttg atgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
    11941 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat
    12001 caaagctgac gccatcaacg ctccgctttc tgccaactca ttcttgccac aaccacaccc
    12061 aggcaactgt ggcaaaacct ttgtgattga taaagcaggt tattaatcct ctgatttaca
    12121 aaacacgcta attcgtcttc cataaaccat gacgattaag atttatacat aatgcgctac
    12181 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
    12241 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
    12301 tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
    12361 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
    12421 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
    12481 tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
    12541 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt
    12601 ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact
    12661 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
    12721 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
    12781 gaggatgcac tcgcatcctc tttccgctca aatatcaacc agatttatca ttttgctctt
    12841 aagcgcagca ttctaatctg aactggttat tcatctgcgg aacctcatat gagcgaagct
    12901 ctcggtatca ctgcatttta tatattcata gcagcaatta ttgttgctgc agtgctatac
    12961 cttgaacaac gctggtagcg cctggtttta tttattttcc agctcaatag caaattcaga
    13021 tcaaatcagc ctcgcaaatt atagattcat aaccaataat gtgctgttat gtatacatgc
    13081 aaaaacaagg agagaatgct tatgtacaaa acaatcatca tgccagtgga tgttttcgaa
    13141 atggaattga gcgacaaagc gattcgccac gctgaattcc tcgcccagga tgacggagtt
    13201 attcatctac ttcacgtact accggggtca gccagcctga gcctgcaccg ttttgccgct
    13261 gatgtacgtc gttttgaaga gcatctgcaa catgaagcag aagaacgttt gcaaacgatg
    13321 gtcagccact tcaccatcga tccttcccgc attaagcaac atgtccgttt tggtagtgtg
    13381 cgggatgaag ttaacgagtt agcgaaagaa cttgatgcag atgtagtagt gattggttcg
    13441 cgtaacccat caatttcaac tcatctgttg ggttctaacg cctcgagcgt aatccgccac
    13501 gccaatttgc cggtgctggt tgtgcgttaa ttgccagaat aagtctcccg ccctgcccgg
    13561 agccatccct gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc
    13621 taagcaatta caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc
    13681 aaaagcactc agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg
    13741 aaccgtcgta cagtcacccg ctgcgaacac gcctttgacg ttggtttcgc atttcgcatc
    13801 aatgataatc tcgcccatgc gattacgttc gactgcgcct tccagccagt tggtgttcgg
    13861 cagcagacca atctggacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac
    13921 acgatcgcga tattccagac ctacgacttt gctaccgtcg cctttcactt ccgtggtttg
    13981 cgcattcaga ataatgtcga cgtttttcag gctgcgtagt ttgtcctgca gaacctggtc
    14041 ggctttcatt tctggcgcaa attccagcag cgttacgtgc tcaacgatac ccgccaggtc
    14101 aattgccgct tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa
    14161 cagcgggccg tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc
    14221 aggaacgttc atgttgcgcc attttgcacc agtcgccacg ataatgctgc gtgctttcag
    14281 taccgcgcca gaagctgttt caatctgatg caggccgcct tcaaccgctg ccgggatcag
    14341 tttgctcgcg ctctggctgt cgatcacatc aacgtcgtat tcatcaacat gaactttcag
    14401 cgcacctgcc agtttctggc cttcggtctt cggtacagaa atgtagtttt cgatatcaac
    14461 ggtatcgagg atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg
    14521 tgcggagtaa attgctgccg ctgcacccgc agggccggaa ccaacgatta atacgtcata
    14581 agcatcacgc ttgttcagct cttctgccgc acgtttttcc gcaccagtat caattttggc
    14641 aacgatttca gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc
    14701 cggaacgccc atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc
    14761 agtgtgcttg atgcgcgggt tcagtacgct catcaggttc agcgcctgca ccacgtccgg
    14821 gcagttgtgg caagagagcg aataataggt ttcgaattca aaatcaccgt caatatggcg
    14881 aatctgctcc agcagagact gcgcttcttt cgacggatga ccaccggtcc acaacaacgc
    14941 cagtaccagc gaggtgaact cgtggcccag cggggaacct gcaaaacgtg gcccctggtt
    15001 ggaaccaggg ttggtaatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt
    15061 aaaggtgact ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga
    15121 tttagcgctg tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta
    15181 agccttgagt tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat
    15241 catcatgcaa gcggcctctg cggctagctg gatgcagctt gcatcatggt gcggggaaga
    15301 aaatcgggtg ccgcagcacc cgaagaatta ggtgaatttc cgaagtatct cgcgccggag
    15361 ttgatagcgc gaaagacgaa ggaaatttag attttaccaa ccaggtccag agacggagcc
    15421 agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg agaagctacg
    15481 tactgtgctg ctttgatttt acgcagcagg tcagacgcgt cacggccaat gccttcagcg
    15541 gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc
    15601 agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg
    15661 atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg
    15721 aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag ttcttcgtaa
    15781 tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc cgggtagaag
    15841 aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt
    15901 ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat ctatacttcc
    15961 tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta atgcgtttcc
    16021 attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt gaactttgca
    16081 ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt aatctgaact
    16141 accctttggt atcttcagca cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca
    16201 acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta agctcttacc
    16261 tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa aggacaaatt
    16321 aatgttaaaa aagatacttt tactggccct gcttcctgca atcgccttcg cagaggaact
    16381 tcctgcccca gtaaaagcga ttgaaaaaca gggtattaca atcatcaaaa cgttcgatgc
    16441 ccctggagga atgaaaggtt atctcggtaa gtatcaggat atgggcgtca ccatctacct
    16501 gactccagat ggtaagcacg ctatctctgg ttacatgtac aacgaaaaag gtgaaaacct
    16561 gagtaacacg cttatcgaaa aagaaattta cgcacccgcc ggacgtgaaa tgtggcaacg
    16621 gatggaacaa tcccactggc tcctcgacgg taaaaaagac gcgccggtca ttgtctacgt
    16681 cttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggctc gtccgtgggt
    16741 agattctggc aaagtgcaat tgagaacatt gttggttggg gttatcaagc cagaaagccc
    16801 ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc aacaatatga
    16861 agcctctggt ggcaagctta agctaagcgt gcctgcaaac gtaagcacag agcaaatgaa
    16921 agtgttaagc gacaacgaga aactcatgga cgatctgggg gcaaatgtca caccagctat
    16981 ctattacatg agtaaggaaa ataccctgca acaagccgtc ggattacccg atcagaaaac
    17041 gcttaatatc attatgagga ataaataaaa gataattcat ggatggttga aatacatcat
    17101 tgcttttcaa ccatcctaaa aacgcatgaa ttgctcgtaa cccacctgac agctatgcta
    17161 ttttagaaaa tcttaatcca aagttaagca gaaaaacata atacatataa atacaacaac
    17221 attattgatc aagtgaatga tttatggaag agacaataca acggagtatg ttatggctaa
    17281 tctttatgat ctaaaaaaat ttgacctcaa tttactggtt atatttgaat gtatttatca
    17341 acatctcagc attagtaaag cggcagaatc attatatata acaccctctg ctgttagtca
    17401 gtcgttacaa cgtttacgtg cgcaatttaa tgacccgtta ttcattcgct cagggaaagg
    17461 tatcgcgccg actacaacag ggttaaacct gcatcaccat cttgagaaaa atctcagagg
    17521 ccttgaacaa acgatcaata tcgttaataa gtctgaacta aagaaaaatt ttattatcta
    17581 cggtcctcag cttatttctt gtactaataa caacatgcta atccgctgtc ttagacaaga
    17641 tacttcaatc gatatagaat gccatgacat actgctttca gctgaaaatg ccgaagaact
    17701 gttggttcaa cgcaaaactg acctggcaat tacgctgcaa ccagtaatta gtcgctctgt
    17761 catctgcatg ccgttacaca ctattcgcaa ttcccttata tgtagcaaca aacatcctcg
    17821 tattactgat accagtaact atgaacaaat tatcgccgaa gaattcaccc tgttgatatc
    17881 taaatcagca gggattgacg aaattcaaat ggacatagat gaacgtttta taaaccgtaa
    17941 agtttcattt cgcagttctt ctttgttgac tataattaat agcatttctg ttactgactt
    18001 attaggcatt gtgcccaccg aactgtacga tttacatcgt gactttttga agttaaaaga
    18061 aataaaactg gaacaacctc ttcctgcggt taaactttat atttcctata acaaggcttc
    18121 gttgaacaac cttgtcttct ctcgattcat agatcgttta aatgatagct tctgattcat
    18181 taccactgac aaataatata ttacgcatca attaattcat tgtgtgaata taaaataatg
    18241 tgctctatct tgcttcgttt atcctataat tagcggattc atctcacgag tcaccccgta
    18301 acgttcagag agcgttttta gatgtctatt tataaaatcc cgcttccact gaatattctg
    18361 gaagctgcaa aagaacgtat tacctggaca cttaataccc taccccgtat ctgtgtttct
    18421 ttttcgggtg gtaaagattc cggattaatg ctgcatctga cagctgaaat tgcccgacaa
    18481 atgggcaaaa aaatctgcgt tttgtttatc gactgggaag cgcagttctc atgcactatt
    18541 aactatgttc agtcattacg tgagttttac gccgatgtca tcgaagagtt ttactgggtt
    18601 gcgctcccgc ttacgacgca aaattccctt tctcaatacc aacccgaatg gcagtgctgg
    18661 gaacctgatg tcgaatgggt gcgtcagccc ccgcaagatg cgataaccga tcctgacttt
    18721 ttctcctttt accagcctgg catgaccttc gaacaatttg tgcgtgagtt tgctgaatgg
    18781 ttttcacaaa aacgtccggc ggcgatgatg atcggcatcc gtgcagatga gtcctacaac
    18841 cgttttgtcg ccatcgccag tttaaataaa caacgttttg ccgatgataa gccctggacc
    18901 accgccgcac caggcggtca tagctggtac atttacccca tctacgactg gaaagtggct
    18961 gatatctgga cctggtatgc taaccatcaa cagctctgta acccactgta taatataatg
    19021 tatcaggcag gcgtccctct gcgtcatatg cgaatttgcg aaccttttgg cccggagcaa
    19081 cggcaaggat tatggctcta tcacgttatc gaaccggatc gctgggctgc tatgtgcgca
    19141 cgagtcagcg gggtaaaaag tggcggcatt tacgccggac atgataatca tttttatggt
    19201 catcggaaaa tcctcaagcc agaacattta gactggcaag aatatgcatt attgctgctc
    19261 aatagcatgc cggaaaaaac agcggagcat taccgcaata aaatcgccat ttatttgcac
    19321 tggtatcaga aaaaaagcat cacggttcca caaactcagc aaggggacat tggggcgaaa
    19381 gatatcccct cctggcggcg aatatgcaaa gttttactca ataacgatta ctggtgtcgg
    19441 gcattatcat ttagccctac gaaagcgaag aactatcagc gttataacga acggataaaa
    19501 ggaaaacgta aggaatgggg gatactatgc aacaacgatt aacgcaggat ttaacccaat
    19561 ttctcgccag cttgccggaa gatgatcgca tcaacgcgat taatgaaatc cgcatggcga
    19621 tccatcaggt tagccccttt cgtgaagagc cagtggattg cgttctgtgg gttaaaaaca
    19681 gccaacttat gcctaatgat tacaacccaa acaacgtggc accacccgaa aaaaaactgc
    19741 tgaaaaaatc cattgaaata gatggtttta cccaacccat tgtggtcacg catacggata
    19801 aaaatgccct ggaaatcgtg gatggttttc accgccatga aattggtaaa ggctcatcat
    19861 cgttaaaatt acgcttaaaa ggttatttgc cggtgacctg tctggaaggt acgcgtaatc
    19921 aacgcatcgc cgcgactatt cgtcataatc gcgctcgtgg acgtcaccaa atcactgcca
    19981 tgtcagaaat cgtccgagaa ctcagccagt tgggatggga cgataataaa attggcaaag
    20041 agctgggaat ggacagcgac gaagtgttac gcctgaagca aattaacggc ctgcaagagt
    20101 tgtttgccga ccgtcaattt tcccgcgcct ggacggttaa ataactacag ctggcgcagg
    20161 cgttctgctg ctgccagcaa cgtcgattcc ttcttggcaa aacagagacg aatcagttta
    20221 tgtgggaagg gatcggcgca aaacaccgac agcggaatcg ccgctacgcc gtgctcccgc
    20281 gtcagccact ggcaaaactc aacatcatcc atcgtagata cggcgctgta atccaccagc
    20341 aaaaagtatg taccttcgca cggtaaaatc tccagccgac tttcatttaa ggcattcacc
    20401 agaatatcgc gcttctggcg ataaaagtcc ggtaatgcaa gataatgctc aggttctgca
    20461 cgtagcatat cggcaagcgc cagttgtgcc ggggtattca ccgaaaaggt cagatactga
    20521 tgcactttgc ggatctcggc gctgatgggc gcaggcgcaa cgcaataacc cactttccag
    20581 ccggtcatat gataggtctt accaaacgat gacaccgcca ccgctcgctc acgcaattgc
    20641 ggatgcgcca gcacgctggc atggccctgt tgtgaaaaat tgatgtgctc gtagacttca
    20701 tcgctaatga caaaaatctc gtgcccggcg atcgcctgcc acagagcggc gaaatcagtc
    20761 tgctgccaga cagttgcact ggggttatgc ggggtgttga ggatcaccag tcgggtgcgc
    20821 tcgcttaaca atgcggcaaa ttcctgccag tcaacgcgaa aatgcggcgg ttgcagtgca
    20881 atacgtttca ctattccccc cgaaagtgcg atggcggggg cgtaactgtc atagctggga
    20941 tcaaaacaaa tcacttcgtc gccattgcgc accaatgcgg ttatcgccgc gtataacgcc
    21001 tccgtcgccc ctgccgtcac ggtgatattg ctgtcgacat ccggttgata gccatacaga
    21061 cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca cgccggtcat gggtgcgtat
    21121 tggtttgccc cctgggcaac gtggtacgcc agccgctcct gtaaatagcg cggaccatca
    21181 aaatcaggaa agccttgcga aaggttaatc gcctggtgtt gctgcgccag cgcgctcatc
    21241 ttggtgaaaa tagtggtgcc aagttgtgga agtttgcttt gtggaatcag agggttattt
    21301 gtcattttta ttgtacctga cgaagttgcg tgttgaaggc actataacac gatgctagta
    21361 tttggcaatc aagacgttta gatgtctaaa taaaacaata aggacaacac aacatgcctc
    21421 acaatcctat ccgcgtggtc gtcggcccgg ctaactactt ttcacatccc ggaagtttca
    21481 atcacctgca cgattttttc actgatgaac aactttctcg cgcggtgtgg atctacggcg
    21541 aacgcgccat tgctgcggca caaaccaaac tgccgcctgc gtttgaactg ccaggggtaa
    21601 agcatatttt gtttcgtggt cattgcagcg aaagcgacgt gcaacaactg gcggcggagt
    21661 ccggtgacga tcgcagcgtg gtgattggcg tcggcggcgg cgcactactc gacaccgcga
    21721 aagccctcgc ccgccgtctc ggtctgccgt ttgttgccgt tccgacgatc gccgctacct
    21781 gcgccgcctg gacaccgctc tccgtctggt ataacgatgc cggacaagcg ctgcattatg
    21841 agattttcga cgacgccaat tttatggtgc tggtggaacc ggagattatc ctcaatgcgc
    21901 cacaagaata tctactggcg gggatcggtg acacgctggc gaaatggtat gaagcggtgg
    21961 tgctggctcc gcaaccagaa acgttgccgc taaccgtgcg gctgggaatc aataatgcgc
    22021 aggccattcg cgacgtcttg ttaaacagta gcgaacaggc gcttgccgat cagcaaaatc
    22081 agcaattaac gcaatcattt tgcgatgtgg tggatgcgat tattgctggt ggtgggatgg
    22141 tcggtggtct gggcgatcgt tttacgcgtg tggcggcggc ccatgccgtg cataacggtc
    22201 tgaccgtttt gccgcaaacc gagaagtttc tccacggcac caaagtcgcc tacggaattc
    22261 tggtgcaaag cgccttgctg ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc
    22321 agcgttttca tctgccgact acgctggcgg agctggaagt ggatatcaat aatcaggtgg
    22381 agatcgacaa aatgattgcc cacaccctac gtccggtgga atccattcat tacctgccag
    22441 tcacgctgac accagatacc ttgcgtgcag cgttcgaaaa agtggaatcg tttaaagcct
    22501 gacgtacaaa tttagcagca gcgcgcgccg ccttttccac catagcgcgc gtcctgccgc
    22561 tcacggaaaa actcctcgta ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt
    22621 tcgacatagt tgtcgtaatc aggcatacca atcatcagtt tcgccgcctg acctaaatat
    22681 tttccggctt ttgccagtga atcaaacatc taaacctccg gtaatacaaa tgccctcctg
    22741 aattaagagg gcttaattgc tatcaacacg ctcgggctga gagtaagggg aacgttcagc
    22801 acatcccccc tcactctgac tttagtgcgc gcctttcgcc tgcgccacga tctcctcgac
    22861 attttccggc atcggctcat atggcgtttc tttcgccgtt ggcttcggct ctttcaatgc
    22921 cgccagtgcc gtcttaatcg agaacagcgc cagaaccacc acgaccacca taaagaagat
    22981 ggttaacccg gcatcaagac ggttgttgaa caccagttgc gccagttgtg actcagtata
    23041 ctgcgatgga atattgccgc tgtcgatcat tgcctggaac ttgttagcaa tcgccaggaa
    23101 tccgactttc gcatccgggc taaacgcttt ctgccagcct gcggtcaggg tacaaatcag
    23161 cagccaggcc gttggtacca gcgccaccca ggcgtaacgt tgacgtttca ttttgaacag
    23221 caccacagca caaagcatca gcgccatccc tgccagcatc tggttggcaa taccaaacag
    23281 cggccacaag gtgttaatac cgcccagcgg atcgacctcg ccctgatgca ggaagtagcc
    23341 ccacgccagc acgcacagcg ccgttgccag caggttagca ggcagtgaat cggtccgttt
    23401 caggcccgga gacaccacgc ccagcagatc ctgcaacata aagcgcgcag cacgcgtacc
    23461 cgcatccacc gccgtcagaa taaacagtgc ttcaaacaga atggcgaagt gataccagaa
    23521 cgccacatcc atcatgccgc ccagcgcacc gtgcagaatg taggccatcc ccaccgccag
    23581 ggtcggcgca ccgcccgcac gggaaatgat cgactgttca cccacttcgc tggcaatctg
    23641 gtttagcgta tccggggtaa tcgcaaagcc ccagctactc accacctgcg cggcagaagc
    23701 aaccacatcc gccgtccctg ccggagccag caccgccatc gggctgttca tggcgaagta
    23761 cacacccgga tcgatgatac aggcggaaac cagtgccata atcgccacaa aggattccat
    23821 taacatcccg ccgtagccga taaagcacgc ctgcccttcg ttcgccagca tcttcggcgt
    23881 ggtgccggaa gagatcagcg catggaagcc agacaccgcg ccacaggcga tggtgataaa
    23941 caggaacggg aacaggttac cggtccatac cgggccagtg ccatcgacaa atttagttag
    24001 cgcaggcatg gtcagcgtcg ggcgcataat taaaatgccc accgccagac cgacgatagt
    24061 cccgattttc aggaaggttg agaggtagtc acgcggagcc agcagcaacc acaccggcag
    24121 caccgccgcc acaaaaccgt agcccaccag catccaggtc agctgcacgc cggtaaagtc
    24181 aaagtacggt gcccaggtcg ggctttctgc cacccagccg ccagagataa tggcgaaaat
    24241 caggaacacc agaccaatta ccgacacttc gccaatacgc cccggacgca aatagcgcag
    24301 gtagatcccc ataaacagcg ccagcggaat ggtgaacgca acggtatacg ttccccacgg
    24361 gctatgagtc agggctttca ccacaatcat cgccagcacg gcaaggataa tgaccatgat
    24421 cataaagcag gccaccagcg ctatcacccc ggcggttggt cccatctctt ctttgaccag
    24481 ctcacccagc gagcgaccgt cacggcgcgt agaaacaaac agcaccatga aatcctgcac
    24541 cgcaccggcg agaaccaccc cggcgagcag ccagatcatc cctggcaggt aacccatttg
    24601 cgccgccagt accggcccaa ccaacggacc tgctccggca atggccgcaa aatggtgacc
    24661 gaacagcact tttttgtccg tcggcacata gtccagcccg tcgttatggc gcaccgctgg
    24721 cgtcatacgc gtcgggtcaa ccgccagcac atttttggcg atatacaggc cataaaaacg
    24781 gtaggcgatt agatagatac agaccgacgc caccacaatc cacagcgcgt tgatctgttc
    24841 cccacgattt aaagcaatgt atcccagagc aaatgctccc attacagcga gcactgtcca
    24901 gacgaggtat ttccctgatt tgttcatagt tgttatccgt gtgcgtgtcc atagagatgt
    24961 tacattttgt ttctataaca tctttgtaca atttaacaac acagcaacca tgtaaatcgg
    25021 aattgaaacc aatatttaac ttaacaatgt taactcgatc actccgttaa ccgagttttc
    25081 ctgcaatctc atttaattct gtcgggctca tcccaaaact gccgtaccca gccgacaggt
    25141 gcagcagcgc cgcccctgtt catcgaaaac gacaatttcc cagctctgat tttgtcgtcc
    25201 aagatgcagc ggctggcaga caccgcgcac ctttccctca gacaccgggc gatgatgtgt
    25261 ggcgttaagt tccgtgccca ccacacactg tccgtcgcgg gtcatcataa atccggccat
    25321 cgatcccagc gtttccgcca gcgccgccga cgcgccgcca tgcagcaggc caaacggctg
    25381 atgagtacgg gtatcaaccg gcatttcggc ttcgagtaca tcatcgccca gacgggtata
    25441 cacaattccc agatgcgcca ccattgtgtt atcgctggtg gcgttcagct cgtcgagcgt
    25501 taaatgacgt ttccagatca tgcttatgcc cccagcgttg agccgccatc gaccacaata
    25561 tcctgtaggg taatatggct ggcgaggtca gaggcgagga acaaaatcgt gttggcgatc
    25621 tcttgtggac aggcgatttt ccctagcgga atgccgagtt taaactgctc gccaaagccg
    25681 cgaatacgct gctcttcggc gtcatcgctc acccacagcg tgcgttgcat atcggtgtcg
    25741 gtggagcccg gcgaaaccac attacagcgc acgccgctac ccgccagttc cagcccgacg
    25801 ctcaacgcca ggcttttcag cgccgctttc gacgcgccat aagcactcat accaatacgc
    25861 ggcgtgtgtg cggcgtcgga cgcaacagta acaatcgccc cgccccgctg acggcgaaac
    25921 tggttcatgg tctgctgaaa caggttaaac gcaccgccga cgttgaccgc aaaagtctgc
    25981 tgccagtcct ctttactgag ctgatcggtc gcgcccatgc gcaaaattcc cgccgcattg
    26041 accagcacgt ccagtcgctc cgtttcagct aacagtcgct gacatacctg cgccacctgc
    26101 gcagcatcgg caacatccat tacttcggtc gcaaaaggat attgctcctg actaaacgct
    26161 tgatcaaaac ctgtaacttt cgcccccgcc tcaacaaatg ccagcgccgt ggcgtagcct
    26221 atacctttcc ccgcgccagt tacccagaca tttttaccgc tgaaatccat tatttcacct
    26281 cgcgggagag tagcttccac caggcgtcga tggtcgggtt tttcgccagc atgacaaagt
    26341 cgatatcgcc atgcactttg cgccagcgcg ccgccagcgc catcatgcgc accgaatcca
    26401 gaccgtagtc gatcaggttg tcgtcatcga agggttcatc ggactcgtcc agcaacggca
    26461 ggatcacctc acgcagcgcc gctttgctgg cggggatagg tgctggcagc aattcttcgg
    26521 tcatcaccac ccgaccagaa cgtccggcca catatttcag cgacatcaaa tgctcgtcac
    26581 ggctgaaatc ggccagcgca tccgccacca taaacggttt aatatcgcgc ataaatgcgt
    26641 ccgttgcggt ggtcatacag ccaatgtggg catatacccc ggtaataatc agctggttac
    26701 gtccactctc tttgagcatt tgctccagcg gagaacgatg aaatgcgctg tagcgccatt
    26761 tcaccagcac cgtgtcgtcg gcatctggcg tcaggcgatc caccaccttt tgctgttccg
    26821 gcgaacgggt caggccaggc ccccacatat cattcaacag cgcccgatct tcatcgctct
    26881 gctctttcgg ctgggcggtg taataaaccg ggatattgtg ctgtttgcag tagtcgcgta
    26941 gtgccgcaat attcgcgatc acctgctcca tcatcgggca gttctcaccc cagaagctga
    27001 caaaatagtc ctgcatatca tggattaaca acgcggcacg ttgcggttca aaggcccagt
    27061 cgactttatt ctgcggaata tcgtgaggct ccggcagtgc gtaagcctgt aattttggaa
    27121 tagccatcgt gttctctcct tcaggctgat gtgcgtgacg ccagccactg acgtaattgt
    27181 tttttatcga ctttcccgac cgccgtcagc ggaagtgaat ctacacactc cacgcgatcc
    27241 ggtaatttaa attcggcaat accctgttca cgcaggaaac gacgcacctg caccgcgcgc
    27301 agcggctctt ttaccaccag ataagcacag cttttttcgc ccatcagctc atcttccatg
    27361 ctcaccagtg cggcgtagat caccgccgga tggcgcagca gcaggttttc gatctcttcg
    27421 gcagcgatct tctcgccgcc acggttgatc tgatctttct cgcgcccctg cacagtgatg
    27481 taaccctctg gatcaataga gatcagatcg ccggaacagt aaaaaccgtt ggcatcaaag
    27541 gcgctggcat tgtgctgcgg gcttttgtaa tagccacgga aagtgtacgg cccgcgcgtc
    27601 atcaggcgtc caacttcccc ttgcggcagt ggatttcctt cggcatcggc aacccatact
    27661 tcatcgtccg ggcacattgg gtaaccctgg gtatggataa ttttctccgc actatcatca
    27721 agacgggtgt agttcaccag cccttccgcc atgccaaaca cctgctgcaa ctggcagcca
    27781 atctcagcgg gaatacgcgc cgcaagcgtg gcagaaagac gtgcgccgcc gacctgtaac
    27841 agtttcagcg aggcaagctg cgcccggctt tcgccttcag ccagtgcctg caaccacagg
    27901 ctgactgctg gcggcaccag cgcggtgacg ttcacctgat gtttttcaat caatgggaag
    27961 caaagcgtgg cgctggggtc ggcagccaga acgacagtgc cgccagcgag aaataccccc
    28021 aacgatcccg gtgaactcat ggcgtagtta tgagccgccg ggatcgcgca caggtagcgc
    28081 gtctgttgtg tgaactgaca aatctcgacg ctacgacgca cgctgtagta gtagtcgtta
    28141 tgagtgcgcg ggatcagttt cggagtgccg gtggtgccgc cggaaagctg gaaataggcc
    28201 acttcatcag caggagatgg cgtggcagta aaatcctcag ccggatggtt aatcgcatcc
    28261 tgcaagttat gctcaccgct gtcgttgagc agctgcacca cgcgaatgga agaatgctct
    28321 gcaacaaatg tgttgaggaa atcatccccg ctaaatagcg catgttggcg atcggcaatc
    28381 agtaacgccg gttcaatctg gctggcatag gcgttcagtt cactacgctg atggctgaac
    28441 agcgccagca ccggcgcaac gcccagtttc agcagcgcga aaaaggtaat gtaaagttca
    28501 gcgacgttac ccagttgtac cagcgcggtt tcaccaggtt taatgccctg acggcgcaaa
    28561 ctacaggcga ggttatccgc cgcctgattc agctcccggt aactcaactg ccactcgccg
    28621 tcgatcaccg cgatgctgtc actcgcagcg tggcgagtca gaatgtcggt cagtggcaaa
    28681 tcctgccagt agcctttttc ccgatagcga cgggcaaact cttccggcca tcgggtgaat
    28741 ggaatgctca tcctcgctcc ttaatgcaat ccaaaaacgt tcaacatggt agaaagtttg
    28801 acgcctgttt cgcgccactc acccaacggt gaggacgcag gcacaatccc cgctccggca
    28861 aacagacgca cctgattttc ccgcagcttc gcgcagcgga tggtcaccac ccattcaccg
    28921 ttaccttcgc tgtcacacca acccacaatg ccgccaaaca gttcgcggtc gaacggctcc
    28981 agttcagcaa taacctgagt cgcggccaga tgcgggaaac cgctcagcgc aggagttgga
    29041 tgcagcagac aggccagagt cagtgcgttt tcttgcgaat tcgctttacc ttcaaaggga
    29101 gttgcgagat gccacagcgt cggcgtggta atcaattgtg gagaggaggg aacgtgtaac
    29161 tcactactgc gttcgcgcag tacctctttc atcgcctgag tcaccagttc atgttcatgg
    29221 cgatcttttt ctgacgccag cagacgatta cccgcttcgc gatcgagcac ttcatctggc
    29281 tgacgacgcg cggaaccggc taacggaatg gagctaaaac gctcgccgtc tttacgtagc
    29341 agcagttccg ggctggcccc cagcagaacg ccaccatcag ccaacggaac atggaagttg
    29401 taactaaccg ggttttgcgc aatcaaccgt tccagcaata cgccactatc aatggcggcg
    29461 tcagtggtga tatcaatcaa ccgtgacaac accactttgt cgacctgcgg cgtggcggta
    29521 agtgcggcag cacgggcaac catctgttca aacgtggttt gttccggaat ggcctgacgt
    29581 tccaccacgt tcagcgactg gctgcgggtg aaacggcgtg cggaagcttg tttttcctga
    29641 cgggagaacg actgccagga ttcgggaata tacagcgacg aaggctgacg tggatcgaag
    29701 ggaatcgccc cgaccatcac cggatttttg atgccctgcg ctttggcatc ggcaaacagc
    29761 gcggcgagtt tttgctggaa gggactgtcg ggtgaatccc cgttcacagc gggttcatcg
    29821 aagcgggcga aacatcctga cgtcgtaaaa ctgcggtacg gcgacataaa gaaaaagcga
    29881 ttgggcgcaa gtgttgccat ggtctgctgt acttcctcag ccagtgacgt atccatatca
    29941 tcctccacaa aatgataaag gctttaataa tgattatcat ttatattttc ggtcgctaac
    30001 ctaaaagcaa acgcgcacta tgtcaactct tgaggtaacg caatctgcaa ctcggggttg
    30061 catctggttg tggttacaat gaaaatgaga agcattaact tcattaataa caggaagctg
    30121 atttgtgaga ctcgcctcgc tctaccgcaa cgcccttcta ttaacaggac ttttgctttc
    30181 aggaatagcc gcagttcagg ccgccgactg gccgcgtcag attactgaca gccgtggcac
    30241 tcataccctg gaaagccagc cgcagcgtat tgtatccacc agcgtcaccc tgactggctc
    30301 actgctggcg attgatgctc cggtgatcgc cagcggcgcg accacaccaa ataaccgcgt
    30361 agcggatgac cagggctttt tacgccagtg gagcaaggtg gcgaaagaac gcaaactgca
    30421 acggctctat atcggcgaac cgagtgcaga agccgttgcc gcgcaaatgc cggatctgat
    30481 tttaattagc gcaaccggcg gcgattcggc gctggcgctg tacgatcagc tttctaccat
    30541 cgccccaaca ttaatcatca attacgacga caaaagctgg cagtcgctgt taacgcagct
    30601 tggcgaaatt accgggcatg agaaacaagc ggcagagcgg attgcactgt ttgataagca
    30661 actggcagcg gcgaaagagc aaatcaaatt accgccgcag ccggtcactg cccttgtcta
    30721 taccgccgct gcacacagtg ccaatctctg gacgccggaa tcagcacaag ggcagatgct
    30781 ggaacaactc ggctttacgc tggcgaagtt gcccgcaggc ttaaacgcca gccaaagcca
    30841 gggcaaacgc catgacatca ttcagcttgg tggagaaaat ctggctgcag ggttaaatgg
    30901 cgagtcacta ttcctgttcg ccggtgatca gaaagacgcc gatgctattt atgctaatcc
    30961 gctgctcgcg cacctgcctg cagtacaaaa caagcaggtt tatgcgctgg gaaccgagac
    31021 attccgcctg gattactaca gcgccatgca agtgctggaa cgacttaagg cgctgtttta
    31081 agcattaatt gtcggacgct gtcacctgcg gcggcgtctg gcgaaaacgc cgcaactcca
    31141 ccagcaccag caacaacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    31201 aagcaaccgg tgtcatcatc gctcccagac cgcctaacag cgccgcacct atggcatcgc
    31261 ccgtcacgtt ttgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    31321 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagtg
    31381 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    31441 acgcccccaa tgtggagagc aacatcaaca gacctggccg tacgctatgc gccagttttc
    31501 cactggttaa cgcgccaata gccgcaccga gcgggattgc cgcatagaga aagccaattt
    31561 gcgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    31621 ccatcgtcaa taaacccccc agcaacgcaa tccctcccac cagcgggctg gcgagtagaa
    31681 aacgaaatcc tgccagtaat gatttcaacg gatgctcacg cggctgcggc ggtggtggca
    31741 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    31801 agttccaggc tacgcctccg gtcgccagca gtaaaccacc aatcatgggc gaaatcaccg
    31861 accccagacg cacagtcaac atggtgatcg ccccggcctg cattaggttt tcacgtccta
    31921 ccagtgcagg tgtcgccgcc agtagcgccg taacgccaag tgatgcgaaa aaaccatccc
    31981 ataaaccaag taaatagatt gccagcaaag acggctccgg cagcagcgca ttcaggcaca
    32041 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    32101 agcgatccgc cagcacacca ccgatcatca gaccgacaaa catagcaccg ccggtcagcg
    32161 tcaccgaaag ccccacctgc caggtagaat gtgtcatcat ctggatctgc accggcaccg
    32221 cgacgctgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    32281 acgccgggtg cgtttttaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    32341 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    32401 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    32461 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    32521 ttaagcctgc tcattggagc aaaatcactc cccgcttccg tagtgctgga agccctctcc
    32581 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cacgactgcc gcgcaccctt
    32641 gccggtttac tggcaggcgg tgcgcttggc cttgccgggg cgttaatgca aaccctcacc
    32701 cgaaacccac ttgccgaccc cggcttgctt ggcgtgaatg ccggagccag ctttgccatt
    32761 gtgctgggtg cggcgctgtt tggttactct tccgcgcagg aacaactgat gatggccttc
    32821 gccggcgcgc tggtggcgtc attgattgtt gcctttaccg gcagccaggg cggcgggcag
    32881 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaaggactg
    32941 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    33001 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggtgcttacc
    33061 gccggagcaa ccgcgctatt actgagccgc gcgctgaaca gtttaagcct tggcagtgac
    33121 accgcgacgg cgctgggcag tcgcgtagcg cgcacacagt tgattggtct gctggcaatt
    33181 accgtgcttt gtggtagtgc aacggcggta gttggcccga ttgcctttat tggcctgatg
    33241 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    33301 ctactcgcta cccctgccct gctgctgttt gccgatatca ttgggcgggt gattgttccc
    33361 ggcgaactgc gcgtttctgt agtcagtgcc tttattggtg caccagtgct gatcttcctc
    33421 gtacgacgta aaacgcgagg tggcgcatga tttacgtctc tcgccgatta attatcacct
    33481 gtctactgtt gctaatagct tgtgtaatgg caggcgtgtg gggattacgc agcggtgccg
    33541 tcacgctgga aacctcgcag gtattcgccg cgctgatggg cgatgcgccg cgcagtatga
    33601 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    33661 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    33721 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    33781 gtcaggatct gacggctatc gcgctggcag caatggcggg gggtattgtc acctcgctgc
    33841 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggta
    33901 tcggcgttcg cgccatgctg gtggccttta atacctggct gttgctgaaa gcgtctttag
    33961 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    34021 aaacctcacc ttccgcaccc atcattatat taatgttaat tgccgccgcc ttgctggtgc
    34081 ggcgaatgcg cttgctggaa atgggcgatg ataccgcgtg tgcgctgggc gtcagcgtcg
    34141 aacgttcgcg gctgttaatg atgctggtcg ccgtagtgct taccgcagct gccactgccc
    34201 tggcggggcc aatttccttt attgctttag tcgcgccaca cattgcccgc cgcatcagtg
    34261 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg ggcactttta ctgctggcgg
    34321 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    34381 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    34441 cgaatcagta gcccgtttgc gtggcgaaca gttaaccctc ggatatggca aatataccgt
    34501 tgcggaaaat ctgactgtag aaatacctga tggtcacttc acggcaatta tcgggccaaa
    34561 tggctgcggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    34621 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagagg ttgcacgccg
    34681 gattggtctg ttggcgcaaa atgctaccac accgggcgat atcaccgtgc aggagctggt
    34741 ggcgcgtggg cgttacccgc atcaaccgct gtttacccgc tggcgcaaag aagatgaaga
    34801 cgcggtaaca aaagcgatgc aggccacggg aataactcat ctggcggatc aaagcgtgga
    34861 caccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctgg cccaggaaac
    34921 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    34981 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    35041 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    35101 tgttgctcag ggagcgccga aagagattgt cactgctgaa ctgatcgagc gcatttatgg
    35161 tctgcgctgc atgataattg acgatccggt tgccggaacg ccgctcgtgg tgccgctcgg
    35221 acgaacggca ccttcaaccg caaagattta aactaagtgg tctgccatca tggcatcctg
    35281 ttttctggat gccatcgcat gacgcaacaa cacaccacca caagccacca tcccgccgac
    35341 caacgcagaa aggatcacga taatcgcctt accctgaccg tcttttttta cgggtaatga
    35401 cggacgcaac tgatatttaa acggtgtaaa attcacatcg ttgacatttg tttttgttaa
    35461 ttgctcgact agatactgac gattacgtaa ttcaccgttc aattccgcaa cgtcagtgac
    35521 tgctttttca atttccagtt tgcgttcaat accgtctgca ccgagagaaa tagagaaatc
    35581 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    35641 ggcaatgtca agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    35701 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    35761 gacgttttct atcgactctt
//
LOCUS       DAOFSV010000038        33854 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.038, whole genome shotgun sequence.
ACCESSION   DAOFSV010000038 DAOFSV010000000
VERSION     DAOFSV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.038"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..527)
                     /gene="ybtQ"
                     /locus_tag="QBH27_001961"
     CDS             complement(<1..527)
                     /gene="ybtQ"
                     /locus_tag="QBH27_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDQ7014274.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLL"
     gene            complement(514..2226)
                     /gene="ybtP"
                     /locus_tag="QBH27_001962"
     CDS             complement(514..2226)
                     /gene="ybtP"
                     /locus_tag="QBH27_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDQ7014275.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVVRLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            2483..3442
                     /gene="ybtA"
                     /locus_tag="QBH27_001963"
     CDS             2483..3442
                     /gene="ybtA"
                     /locus_tag="QBH27_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDQ7014276.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHSGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFTPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            3633..9740
                     /gene="irp2"
                     /locus_tag="QBH27_001964"
     CDS             3633..9740
                     /gene="irp2"
                     /locus_tag="QBH27_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDQ7014277.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDTYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            9828..19319
                     /gene="irp1"
                     /locus_tag="QBH27_001965"
     CDS             9828..19319
                     /gene="irp1"
                     /locus_tag="QBH27_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HDQ7014278.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLDALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLTPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            19316..20416
                     /gene="ybtU"
                     /locus_tag="QBH27_001966"
     CDS             19316..20416
                     /gene="ybtU"
                     /locus_tag="QBH27_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDQ7014279.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            20413..21216
                     /gene="ybtT"
                     /locus_tag="QBH27_001967"
     CDS             20413..21216
                     /gene="ybtT"
                     /locus_tag="QBH27_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDQ7014280.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRNITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            21220..22797
                     /gene="ybtE"
                     /locus_tag="QBH27_001968"
     CDS             21220..22797
                     /gene="ybtE"
                     /locus_tag="QBH27_001968"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDQ7014281.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENLAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            22928..24949
                     /gene="fyuA"
                     /locus_tag="QBH27_001969"
     CDS             22928..24949
                     /gene="fyuA"
                     /locus_tag="QBH27_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDQ7014282.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            25599..26348
                     /locus_tag="QBH27_001970"
     CDS             25599..26348
                     /locus_tag="QBH27_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HDQ7014283.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSIYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDSDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            26275..28397
                     /locus_tag="QBH27_001971"
                     /pseudo
     CDS             26275..28397
                     /locus_tag="QBH27_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(28567..29619)
                     /locus_tag="QBH27_001972"
     CDS             complement(28567..29619)
                     /locus_tag="QBH27_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDQ7014284.1"
                     /translation="MFLVSLLRRIAFSYYDYKTYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            29934..31250
                     /gene="shiA"
                     /locus_tag="QBH27_001973"
     CDS             29934..31250
                     /gene="shiA"
                     /locus_tag="QBH27_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDQ7014285.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            31352..32806
                     /gene="amn"
                     /locus_tag="QBH27_001974"
     CDS             31352..32806
                     /gene="amn"
                     /locus_tag="QBH27_001974"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDQ7014286.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            33149..>33854
                     /locus_tag="QBH27_001975"
     CDS             33149..>33854
                     /locus_tag="QBH27_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ7014287.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNV"
BASE COUNT         7662 a         9645 c         9067 g         7480 t
ORIGIN      
        1 agcaacatca ccagccccag ccgccagtct atccacaatg tcgccagcga cgccgtcagc
       61 ggcgtgacaa tggtgagcag caaaatattg gctatcgcaa taacataatt gaggttttca
      121 tccacgctgc ccagcagcaa ggcgttcatt tcacccgccc tgccgcgctg gagcttctcc
      181 agcggcacgc ggcgtaactg ctcgccaagt cgcaggcgca actcatgggt agcctgcgcc
      241 agatgaccac ggtattcaaa gcccaggcca taccagcgta gcaccagcgt cacaattgcg
      301 gcgacgctga aggccatagc ccagttaaga agttgcggcg cgtctccccg taacagcgca
      361 tcaatgatcg gataaagaca ggcaaaggcg atcccctgca tgaatgccgc cagcaacagc
      421 gccagcatac tgcgtcgcag catcctggcc tgactgccaa cgctggagat aagctggcgc
      481 cagatgacgc gccaggccag gttatccgca ggattattgt ctttcatttt ccatcacctc
      541 ttcctgtgtt ccacccggca cccaatgctg cgcctgctga tagtgttgcc agagccgttg
      601 atacaggccg ccctgcgcca acagttgcgt atggttcccc atttccctga gctgtccgtc
      661 ggaaaacagc aatatcacat cggcctgagt caccattgag agacgatgcg cgaccatgat
      721 gaccgtccgg ccacgcatgg ccgccgcgag cgccttgata agcgccgctt cgttttccgg
      781 gtcagcaaac gccgtcgcct catcgagcac caggatgggg cgatcctgca aaagcgcccg
      841 ggcgatagta atgcgctgcc gctggccgcc ggagagaaat accccacgtt ccccgactcg
      901 agtgttgtaa ccttctggca gagcgctaat aaaatcatgg gcctgcgcca ctctggccgc
      961 cgctataacc gcctccagcg gcgtatccgg cgcgcccaga cgaatgttat tggctatcgt
     1021 gtcggcaaaa aggaagttgt cctgaaacac aaacgagagt tgcttcatca gggtgtccgt
     1081 ctgcatatca cgcagatcca cgccgccaat acggatatgg cctttgtccg ggtcggcgta
     1141 acgcagcagc aagcgcacca cggtgctttt tccggcgccg cttggcccga ccagcgccac
     1201 aatttgcccg gcaggcacat gaaagctcac ctcctgcaac gcggcgccag tacgcgcttg
     1261 cggataatga aagctcacct gctcaaaggt aatgctcgcc tcttgcggct gctggtcaga
     1321 ctgcggcagc gataactccg gcatcgcgag aacctgataa atacgctgaa tgcttaaacg
     1381 cgtttgcgcg accaggttat tgagcatcat cattggcatt acggcttcgg ccattccgct
     1441 gcccagcagt aaaacggcca cccacgcgat aaaatcgaaa ctgccatagt gcaacaggcc
     1501 gtatcccgac cagatcagga caaacagggt cgggagagga ttcagaatcg agaaggaaaa
     1561 acgcgctgaa aaaccggctt tgcgatacca ggttttgagc acatcgaccc actcttcaag
     1621 ggcgcgttga tagcgcaaaa aactggtgct gccgctatca aacgttctca ccacgggcat
     1681 ggcctgcacg aactcaataa ccgccgcgct aacctgctcg cgcgccttat gataacgctg
     1741 agccatatct tccgaacgct ggcgggcgag cacgagaaca accgatccaa acgccagtac
     1801 ccccagcgtc gcgattgcca gccgccagtc cagccagaac aagataacga ttgtcgccag
     1861 cggcatgatg atcgcgcggg catagagcgg cgtgctgtcg gccacaaaaa tatgtaacga
     1921 cttcacgtca tccagcatca ctttcgccac tgacccgctg cccatttgct gtaacacgcc
     1981 aggcggaagc tgcaatgctt tacgggctaa cctgctgcgc agaatgttct ccagatgaaa
     2041 agccgcgtaa tgagaggtgt tgaacgcctg taagcgcaga acaaaagtta agaccacgca
     2101 gccgatcacg ccgcccagcg gccaggccgg aatagcgtca ggcgtcgcgc gaatgtcacg
     2161 caggctccac gccagaaaaa ggagcgcccc caggctggtt aatgccgcca gccccgccag
     2221 cgccatagct aaaataacct tcctgcgaac cggtgtaatc acctgccaaa gaggaaatcg
     2281 ggacagcgat tcagtattgg atgattgaga tgacatggga gtaactgaat ttcctgatga
     2341 atttttacgg acgccaaaaa cgacaattca gggtggcgtt ctgagaatta atgtgaataa
     2401 taaccattat caataactca tctaccccat tcggttctat ttatacccgc attggtctaa
     2461 gccacaggga gataaccagg tcatgacgga gtcaccgcaa acgcaatctg aaatctctat
     2521 tcaccagttg gtggtcggga aacctgcaaa cgatggcaat attcctgctc agtgtgaact
     2581 gctgcgctgt tcgttgcagg aaggtatgga tattttgctc tggaggggcc attttgcgcg
     2641 gcctgagacg ttgcagctac acgacgatct gggcaggatt aacttctcgt gcatcctgga
     2701 aggcacatcg cgctttgcga ttcagggact ccggcgtcac accgactggg agcttgcgcg
     2761 caatcgccat tacattaccc atacgccaga ctgtcgcggc agcgcctcct attgtggccg
     2821 attcgagagc attaccctct ccttcagccc tgaaaccctc gcgctgtggg tgccggatat
     2881 tagcgcggtc attaagaata agatcgactc ccactgctgt tgccagcagc accgttgtaa
     2941 tgctgaaacc cacttaacgg ctcaggcctt gcgtcacgcg ctgatgcgca tgcacagcgg
     3001 cttcagtcat gaacaaaagc cgtcgacgct atggttgcag gggcaaagtc tggtcatgct
     3061 cagtctggtg ctggatgaac atcgcgaaga cgccagttgc ctctcctgcc acttcacccc
     3121 gatggaacgc cagaaactgc tgcgggcgaa ggatctgctg ctggctgatc tgacgcaagc
     3181 gccgggcgtc gcggagctgg ccagagagtc cggtctgagc gttctcaaaa ttaagcgagg
     3241 cttccgcgtt ctgttcaaca acagcgtcta tggcctgttt caggctgaac gaatgcagga
     3301 agccagaaga cgcctggcca acggcaatac ctccgtcatg acggtggcgg ccgatctggg
     3361 ctatgccaac gccagccatt tctctgccgc cttccagaaa cagtttggcg taactccttc
     3421 gacctttaaa cgcgggatgt aaccggggtc gcgcccccct aaactcctcc ctgacagagg
     3481 cccggatacc ccatcagggc ctctgttctt tccgcctgtt accaacccga aacgggcata
     3541 aataaacccg tttcgggtag catccaattg ttaataatta ttattctcat atgagtaatg
     3601 cttttcggta agacgtgcca tcaggaggaa gaatgatttc tggcgcacca tctcaggatt
     3661 cgctgttacc ggacaaccgc cacgcggctg attaccaaca attacgcgag cggctcatac
     3721 aggaactgaa tttaacgccg cagcagttac atgaagagag caacctgatc caggccggcc
     3781 tggattccat aagattgatg agatggttac actggtttcg taaaaatggc taccgcctta
     3841 cccttcgcga gctgtatgcc gcccccacgc tggcggcatg gaaccagtta atgctcagcc
     3901 ggtcgccgga gaacgcggaa gaagaaacgc cgcccgacga atcatcctgg ccgaacatga
     3961 ccgaaagtac ccccttccca ttgacgccag tacagcacgc ctacctgacg ggccgcatgc
     4021 cggggcagac gcttggcggc gtgggttgcc acctgtatca ggagtttgaa ggccattgtc
     4081 tgacggcgtc gcagctggag caggccatca cgaccttgct gcaacgccac ccaatgctgc
     4141 atatcgcctt tcgccccgac gggcagcagg tctggctacc gcaaccttac tggaacggcg
     4201 tcaccgttca tgatttacgc cataacgacg ctgaaagccg ccaggcctat ctggacgcac
     4261 tgcgccagcg cctgagccac cgtcttttac gcgtggaaat cggcgaaacg tttgattttc
     4321 agctgacgct cttgccggac aatcgccacc gcctccatgt caatattgac ctgctgatta
     4381 tggatgcctc cagctttacg cttttcttcg atgagcttaa cgccctgctg gccggagaat
     4441 cgctgccggc tatcgacacc cgctatgatt tccgctcgta tttgctgcac cagcagaaga
     4501 tcaatcaacc actgagagac gacgcgcgcg cttactggct ggcgaaagca tcgacgcttc
     4561 cccccgcgcc cgtcttgccg ctggcctgcg aacccgccac gctacgtgaa gtccgtaata
     4621 cccgacgccg catgattgtc ccggcaacac gctggcacgc ctttagcaac cgggccggcg
     4681 agtatggcgt gacgccgaca atggcactgg cgacctgttt ttctgccgtg ctggctcgct
     4741 ggggcggcct gacgcgtctg ctgcttaaca tcaccttatt cgaccgccag ccgctgcacc
     4801 cggcggttgg cgcgatgctt gccgacttca ccaatattct tctgctggat accgcctgcg
     4861 atggcgatac cgtcagcaac ctggcgcgta aaaaccagct cacgtttacg gaggactggg
     4921 agcatcgcca ctggtccggc gtcgaattac tccgtgaact caaacgccag cagcgctacc
     4981 cccacggcgc cccggtggta tttaccagca atctggggcg ttccctctac agcagccgcg
     5041 cagaatcgcc gttgggcgag ccggaatggg gcatctcgca aacgccgcag gtctggatag
     5101 atcatctggc gttcgagcat cacggcgagg tctggctaca atgggacagc aacgacgcgc
     5161 tgttccctcc ggcgttagtc gaaacattgt tcgataccta ctgccagttg attaaccaac
     5221 tctgcgatga cgaaagcgcc tggcaaaagc cgttcgcaga tatgatgccc gccagccagc
     5281 gcgcgatacg cgaacgggtc aacgccaccg gcgcccccat tcccgaaggc ttgctgcatg
     5341 aaggcatttt ccgtatcgct ctgcaacagc cgcaggcgct ggcggtaacg gacatgcgtt
     5401 atcagtggaa ttatcatgag ctgacagact atgcccgccg ttgcgcgggc aggttaatcg
     5461 agtgcggggt tcagcccggc gataatgtgg ctatcacgat gtcgaaaggt gcaggacaac
     5521 ttgttgcggt tctggccgtc ctgctggctg gggcggttta cgttccggtt tcgctggatc
     5581 agcctgccgc acggcgcgag aaaatctacg ctgacgccag cgtccggctg gtgctcattt
     5641 gtcagcacga cgccagcgcc gggtcagacg atattcccgt ccttgcctgg cagcaggcca
     5701 ttgaggcgga gccgatcgcc aacccggtag tacgcgcccc cacgcaaccg gcctacatta
     5761 tctacacctc cggctctacc ggtacgccga aaggggtagt catttctcac cggggagcgc
     5821 ttaacacctg ttgcgatatc aatacccgct atcaggttgg cccgcatgac agggtgctgg
     5881 ccctctccgc cctacatttt gatttatcgg tttacgacat ttttggcgta ctgcgcgcgg
     5941 gcggcgcgct ggtgatggtg atggaaaatc aacggcgcga tcctcacgca tggtgtgagc
     6001 tgatccagcg ccatcaggtc acgctctgga acagcgtccc ggcgctgttc gatatgctgc
     6061 tgacctggtg tgaaggtttc gccgacgcca cgccggaaaa cctgcgcgca gtgatgcttt
     6121 ccggcgactg gatcgggctt gacctccccg cccgttatcg ggccttccgg ccacaaggac
     6181 aatttatcgc gatgggcggt gccaccgagg cgtctatctg gtctaacgcc tgcgaaattc
     6241 acgacgtccc cgcccactgg cgctccatcc cttacggttt tccgctaacc aaccaacgct
     6301 accgggtggt ggatgaacag ggccgggact gccctgactg ggtgccgggt gaattatgga
     6361 ttggcggcat tggggtcgcg gaaggctatt tcaacgatcc cctgcgtagc gagcagcaat
     6421 ttttgacgct cccggacgag cgctggtatc gcaccggcga tctcggctgc tactggccag
     6481 atggcacaat cgagttcctc ggtcgtcgcg acaagcaggt caaagtcgga ggatatcgca
     6541 tcgagctggg cgaaatcgaa agcgcgctca gccagctggc gggggtgaaa caagcaaccg
     6601 ttctggcgat cggcgaaaaa gaaaaaacgc tggcggcata cgttgttcct cagggcgagg
     6661 ctttttgcgt taccgatcat cggaacccgg cactgccgca ggcgtggcac acgcttgcgg
     6721 gaacgttgcc ctgttgcgcc atctcgccag agatctccgc agaacaggta gccgatttcc
     6781 ttcagcatcg cctgctaaaa ctgaagccgg gtcacaccgc tggcgccgat cctctccccc
     6841 tgatgaactc actcgctatc cagccgcgct ggcaggccgt ggtggaacgc tggttagcat
     6901 ttctggtgac acaacggcga ctgaagcccg ctgctgaagg ttatcaggtc tgcgctggtg
     6961 aagaacgcga ggatgagcac ccgcacttca gcggacatga tttaacgtta tcgcaaattc
     7021 ttcgcggtgc ccgtaacgaa ctgtcgttac tgaacgacgc gcagtggtcg ccggaaagcc
     7081 tggcctttaa ccatccggcc agcgccccgt atattcagga actggcgaca atttgccaac
     7141 agcttgcaca gcgcttacag cgcccggtgc gcctgcttga ggtgggaacc cgcactggcc
     7201 gcgccgcaga atcgctgtta gcacagctca acgccggaca gattgagtat gtcgggcttg
     7261 agcagagcca ggagatgctg ctgagcgccc ggcagaggct cgccccctgg cctggcgccc
     7321 gtctgtccct ctggaatgca gacacgctgg cggcgcacgc tcactcggcg gacattatct
     7381 ggcttaataa cgccctgcat cgtctgctgc cggaagatcc cgggctcctt gcgacattac
     7441 aacagcttgc cgttcccggc gcgctgctct acgtgatgga gtttcgccag ttaacgccgt
     7501 ccgccctact cagcacgctc ctgttaacca atgggcagcc ggaggccttg ctgcataaca
     7561 gcgccgactg ggcggcatta tttagcgcgg ccggcttcaa ctgtcagcat ggcgatgagg
     7621 tcgcggggtt acaacgcttc ctcgtacaat gtcctgacag gcaggtgcgc cgcgatcccc
     7681 gtcaacttca ggccgccctc gccgggcgtc tgccggggtg gatggtgccg caacggatcg
     7741 tcttcctcga cgccttaccg ctgacggcta acgggaaaat tgactaccag gcgctgaagc
     7801 gtcgtcatac ccctgaagcg gaaaacccgg ccgaagcgga tttaccccag ggcgacattg
     7861 aaaaacaggt tgccgccctc tggcagcaac tcttatcaac tggcaatgtc accagagaaa
     7921 ccgacttctt ccagcaaggc ggcgatagcc tgctggcgac ccgtctgacc gggcaacttc
     7981 atcaggcagg ttatgaagcg caattaagcg acctgtttaa tcatccccgg ctggcggatt
     8041 ttgccgccac gctgcggaaa accgacgtcc cggtcgaaca accattcgtc cactcccctg
     8101 aagatcgcta ccagcccttt gcgcttaccg acgtgcagca ggcttaccta gtggggcgtc
     8161 agccgggctt tgccctgggc ggcgtcggct cacatttctt tgttgaattt gaaattgccg
     8221 atctggatct cacccggttg gagacggtct ggaaccgatt aatcgcccgc cacgatatgc
     8281 tacgcgccgt cgtgcgtgat ggacagcaac aggtgctcga acagacgcct cgctgggtaa
     8341 tacccgcaca catcctccat acgcctgaag aggcgttgca ggtgcgcgaa aaactggccc
     8401 atcaggtact caaccccgaa gtctggccgg tattcgatct ccaggtcggt tacgttgacg
     8461 ggatgcccgc ccgcctgtgg ctgtgtctgg ataacctgtt acttgacggg ctgagcatgc
     8521 agattctgtt ggcggagctg gagcacggct accgctaccc gcaacagctg cttccgccgc
     8581 tgcccgtcac cttcagggat tatctgcaac aaccctcgct acagtcgccc aatccagatt
     8641 ctctggcatg gtggcaggcg cagcttgatg atattcctcc ggcgcctgcg ttgccgctgc
     8701 gctgcttgcc tcaggaggtt gaaacaccgc gcttcgcccg cctgaacggc gcactggaca
     8761 gcacgcgctg gcatcggctg aaaaaacggg cggctgacgc ccatctcacc ccgtcggccg
     8821 tactgttgtc ggtgtggtca acggttctct ctgcatggag tgcacagcct gagttcacgc
     8881 ttaaccttac gcttttcgac aggcgaccgc tgcacccgca aatcaaccag attctgggcg
     8941 atttcacctc gctgatgctg ctgagctggc atcccggcga aagctggctg cacagcgcgc
     9001 agtcactaca gcagcggctg agccagaacc tcaaccaccg cgatgtgtca gccatccgcg
     9061 tgatgcgtca actggcgcaa cggcaaaacg tgccagccgt tccgatgccc gtcgtcttta
     9121 ccagcgcgct gggctttgag caggataact tcctcgcccg gcgtaatctg ctcaaaccgg
     9181 tctggggcat ctcccagacg ccgcaggtct ggctcgatca ccagatttat gaatccgaag
     9241 gcgaactgcg ctttaactgg gattttgtcg ccgcgctgtt tcctgccggg caggtggagc
     9301 gccagtttga acagtattgc gcattgctaa accgaatggc cgaggatgaa agcggctggc
     9361 aactgccgct cgccgcgctg gtgcctcccg ttaaacacgc agggcaatgc gcagagcgct
     9421 caccgcgcgt atgccctgag cactctcagc cacacattgc ggcggacgag agcaccgtca
     9481 gcctgatttg cgacgccttc cgcgaggtgg ttggcgagtc tgtcacgccc gcagaaaact
     9541 tctttgaggc gggcgcaacg tcgctgaatc tggtgcaact gcacgttttg ttacaacgtc
     9601 acgaattttc caccctgacg ttgcttgacc tcttcaccca cccttctcct gctgccctgg
     9661 ccgattatct ggccggcgtc gccacggtgg agaaaacaaa acgacctcgc cctgttcgcc
     9721 gtcgtcagcg gcggatatag cgcgaagcaa actgattttc cccggaacgc catcgcgaac
     9781 gcatggcgtt ccattgactt tgtgaatctt aggaaacggg accgattatg gataacttgc
     9841 gcttctcttc tgcgccgaca gcagattcca ttgatgcatc gatcgctcaa cactacccgg
     9901 actgcgaacc tgtcgcggtt atcggctacg cctgccattt tcctgaatcg ccggatggcg
     9961 aaacgttctg gcaaaatctg ctggaaggtc gtgaatgcag ccgacgcttt acgcgcgaag
    10021 agcttctggc cgtcggtctg gatgccgcca tcattgacga tcctcattat gtcaatatcg
    10081 gtacggtgtt agacaacgcc gactgcttcg acgccaccct gtttggctat tcgcgacagg
    10141 aagcggagtc gatggacccg cagcagcgcc tgtttttgca ggcggtctgg catgcgctgg
    10201 aacatgccgg ttatgccccc ggcgccgtcc cccataagac cggcgttttc gcctcttccc
    10261 ggatgagtac ctaccccggt cgcgaagcat tgaacgtgac agaagtcgcg caggtaaaag
    10321 gtctgcaatc tctgatgggc aatgataaag actatattgc cacccgcgcc gcgtacaaac
    10381 tcaacctgca cggcccggcg ttatcggtac agaccgcctg ctccagctcg ctggttgccg
    10441 tgcatctggc ctgtgaaagc ctgcgcgcag gcgaatccga tatggcggtt gccggcggcg
    10501 tggcgctctc tttcccccag caggcaggct accgctacca gcccggaatg attttctctc
    10561 ctgatggtca ctgtcgtccc tttgacgcct cggctgaggg cacctgggcc ggtaacggtc
    10621 tcggctgcgt ggtgctgcgt cgcctgagag acgcgctgct gtcaggcgat ccgattatct
    10681 cggtgatcct ctccagcgcg gtcaacaacg acggcaacag aaaggtcggc tataccgccc
    10741 cttccgtcgc agggcaacaa gcagtcatcg aagaggcgtt aatgctggcg gccatcgacg
    10801 acaggcaggt aggttacatt gaaacccacg gcaccggcac accgctgggc gacgcgattg
    10861 aaattgaagc gttacgcaac gtctatgcgc ctcgcccgca ggatcagcgc tgtgcgctcg
    10921 gttccgtgaa aagtaacatg ggccatctgg ataccgcggc gggcattgcc ggactgctga
    10981 aaaccgttct ggcagtcagt cgcgggcaaa ttcctccctt actgaatttt cacaccccca
    11041 acccggcgct gaaacttgaa gagagcccct ttaccatacc ggtgtcggca caggcatggc
    11101 aggacgaaat gcgctatgcg ggcgtctcct cctttggtat tggcggcacc aactgccata
    11161 tgatcgtcgc ctcgctgccc gacgcgctca acgcgcgcct ccccaatacg gatagcggca
    11221 gaaaaagtac cgcgctgctg ctcagcgccg ccagcgacag cgcgttgcgg cggctggcga
    11281 cggattatgc cggggcgctg agagagaatg cggatgccag ctctctggcc ttcacagccc
    11341 tgcacgcgcg ccgtctcgat ctccccttcc gcctggcggc gccattaaac cgtgaaaccg
    11401 ccgaggcgct cagcgcctgg gccggtgaga aatcgggggc gctggtttac agcggccacg
    11461 gcgccagcgg caagcaggtg tggctgttta ccggccaggg ctcgcactgg cgcactatgg
    11521 gtcaaacgat gtaccagcac tcaacggcgt ttgccgacac gctggatcgc tgtttttccg
    11581 cctgtagcga aatgctcacg ccgtcactgc gcgaagcgat gtttaacccc gattcggcgc
    11641 agctggacaa tatggcctgg gcgcagccgg cgattgtcgc gtttgaaatc gcgatggcgg
    11701 cgcactggcg tgctgaagga ctgaagccag acttcgccat tgggcattcc gtcggtgaat
    11761 ttgccgctgc cgttgtctgc ggacactata cgattgaaca ggtcatgcca ctggtttgtc
    11821 ggcgcggcgc gctaatgcag cagtgcgcaa gcggcgcgat ggtggcggta tttgcagacg
    11881 aagacacgct gatgccgctg gctcgccagt ttgagctgga tctcgccgcc aacaacggta
    11941 cgcaacatac ggtattttcc gggccggaag cccgtctcgc ggtattttgc gccacgctct
    12001 cgcagcatga cattaactat cgtcgcctga gcgtaaccgg tgcggcgcac tccgctttac
    12061 tggagccgat actcgatcgg ttccaggacg cctgcgcggg actgcacgcg gagccggggc
    12121 aaataccgat tatttccacg ctcaccgccg acgtcattga tgagtcaacg ctcaaccagg
    12181 cggattactg gcgccgacac atgcgccagc cggtgcgttt tatccagagt attcaggtgg
    12241 cgcatcagct cggcgcccgc gtttttctgg agatggggcc cgatgcccag ttggttgctt
    12301 gcgggcagcg cgaataccgc gataacgcat actggatagc cagcgcccgg cgtaacaaag
    12361 aggcgagcga tgtcctcaat caggccctgc tccagcttta cgctgccggc gtcgccctac
    12421 cgtgggccga cctgctggcg ggcgatggac aacgtatcgc tgcgccatgt tatccgtttg
    12481 atactgaacg ttactggaaa gagcgcgtct ccccggcctg cgagcctgcc gacgcagcgc
    12541 tgtctgccgg gctggaggtg gcgagtcgcg ccgcgacagc gctcgatctc cctcgcctgg
    12601 atgcgcttaa acagtgcgcc acgcgactgc acgccatcta cgtcgatcaa ctggtacaac
    12661 gctgtaccgg cgatgccatt gagaacggcg tggacgccat gaccatcatg cgccgtggac
    12721 gtctgctgcc ccgctaccag cagctactcc agcgcctgct gaataactgc gtggtcgacg
    12781 gcgattaccg ctgcaccgac gggcgatacg tccgcgcccg ccccattgaa catcaacagc
    12841 gggaatcact gctgacggaa cttgccggtt attgtgaagg ttttcaggct attcccgaca
    12901 ccatcgcccg tgccggcgat cggttatatg aaatgatgag cggcgcggaa gaaccggtgg
    12961 cgattatctt cccgcaaagc gcctccgacg gcgtggaagt gctgtatcag gaattcagct
    13021 ttggccgcta tttcaaccaa atcgccgccg gggtattacg cggcattgtc cagacgcgtc
    13081 agccccgcca gccgttgcgt attcttgaag ttggcggcgg aaccggcggc accaccgcgt
    13141 ggctgctgcc ggaactcaac ggcgttccgg cactggagta ccatttcacc gatatctcgg
    13201 cgctgttcac ccgtcgcgcc cagcagaaat tcgccgacta tgattttgtg aagtatagcg
    13261 agctggatct cgaaaaagag gcgcagtctc agggtttcca ggcacagtct tacgatctta
    13321 tcgtggcagc gaacgtgatt cacgccaccc gccatattgg ccgcacgctc gataatctgc
    13381 gccccctgct caagccgggc gggcgcctgc tgatgcgcga aatcacccag ccaatgcgtc
    13441 tgtttgactt cgttttcggc ccgctggttc ttccgctaca ggatctcgac gcccgcgaag
    13501 gtgagttatt cctcaccacc gctcagtggc aacaacagtg ccgccacgcc ggattcagca
    13561 aagtggcgtg gctaccgcag gatggcagcc cgaccgccgg gatgagcgaa catatcattc
    13621 tcgccacgct gcccggtcag gcggttagcg ccgtaacatt caccgcgcca tcagaacccg
    13681 tgttggggca ggcgctgacg gataacggtg attatctcgc cgactggtct gattgcgcag
    13741 gtcagcccga acggtttaac gcccgctggc aggaggcatg gcgtctgctt tcacagcgtc
    13801 atggcgacgc tctgcctgtg gaaccgcccc ccgtcgccgc cccggagtgg ctggggaagg
    13861 ttcgcttaag ctggcaaaac gaagcctttt cccgcggtca gatgcgcgtt gaagcccgtc
    13921 atcctgctgg cgagtggctg ccgctatcgc ccgccgcgcc tcttcctgcg ccgcaaacgc
    13981 attatcaatg gcgctggacg cccctcaacg tcgccagcat tgaccatccg cttaccttta
    14041 gcttcagcgc cggtacgctt gcgcgcagcg acgagctggc gcaatacggc atcattcacg
    14101 atccgcacgc ctcttcacga ctgatgattg ttgaggagag cgaggatacg ctggccttag
    14161 cggagaaagt gatagcagcg ctcaccgcca gcgcagccgg attgattgtg gttactcgcc
    14221 gcgcgtggcg agtcgaggaa aatgaagcac tctctgcatc ccatcacgcg ctatgggcct
    14281 tgcttcgcgt cgcggccaac gaacagccgg aacggttgct tgccgccatc gatctcgccg
    14341 aaaacacccc gtgggaaacg ctgcatcaag ggttgagcgc agtctcacta tcacagcgct
    14401 ggctcgccgc acggggtgac accctttggc tcccttcact gacgcccaat acgggatgcg
    14461 ccgctgaatt accggcaaac gtgtttaccg gcgatagccg ctggcatctg gtgaccggag
    14521 cgtttggcgg attaggccgc cttgccgtga actggctcag agaaaaaggg gcgcgacgca
    14581 tcgccctgct ggcaccgcgc gtggatgagt catggctacg cgacgtggag ggcgggcaga
    14641 cgcgcgtctg ccgttgtgat gtgggcgatg ccgggcaact ggccacggtt cttgacgatc
    14701 tggcggccaa cggcggcatt gccggagcga ttcatgccgc tggcgtattg gctgacgcgc
    14761 ccttgcagga gcttgatgac caccagctgg ctgccgtttt cgcggtaaaa gcgcaggcgg
    14821 caagccaact gttgcaaacc ctgcgcaacc acgacggacg ctatcttatt ctctactctt
    14881 ccgctgccgc caccctcggc gcgccgggtc agagcgccca tgcgctggcc tgcggctacc
    14941 tggacgggct ggcccagcag ttttccaccc ttgatgcgcc gaaaacgctc tctgtcgcct
    15001 ggggcgcatg gggagaaagc ggtcgggcgg ccacgccgga aatgctggcg acgctcgcca
    15061 gccgaggtat gggcgcgtta agcgatgccg aaggctgctg gcacctggaa caggcggtga
    15121 tgcgcggcgc cccgtggcga ctggcgatgc gcgtttttac cgacaaaatg cccccgttac
    15181 aacaggctct gtttaacatc agcgccacag aaaaagccgc aacgccggtc attcctcctg
    15241 ctgatgacaa cgcctttaac ggcagcctga gcgatgaaac agcggtgatg gcatggctga
    15301 aaaagcggat tgcggttcag ctaaggctga gcgatccggc gtcactgcat ccaaaccagg
    15361 atctgttgca actcggcatg gactcgctgc tcttccttga actcagtagc gatattcagc
    15421 actacctggg tgtacgcatc aatgcggaac gggcgtggca ggatctgtct cctcatggac
    15481 tcacgcagct tatctgttct aagccagagg cgacgcctgc cgcttcgcag ccggaagtat
    15541 tgcggcacga cgccgacgag cgttatgcgc ccttcccttt gacgcccatt cagcacgcct
    15601 actggctggg gcgaacccac ctcattggct atggcggcgt cgcctgtcac gtcctgtttg
    15661 agtgggataa acgccacgat gagttcgatc tcgccatact ggagaaagca tggaaccagc
    15721 tcatcgcacg ccacgatatg ttgcgtatgg tggttgatgc cgacgggcag cagcgaatcc
    15781 tggcgacaac gccggagtat cacatcccgc gtgacgatct gcgcgcgctt tccccggaag
    15841 aacagcgcat cgcactggaa aaacggcggc atgaactgag ctatcgcgtt ttgcctgccg
    15901 accagtggcc tctttttgag ctggtggtca gcgaaatcga cgattgccat taccgtctgc
    15961 atatgaacct cgaccttttg cagtttgatg tgcagagttt taaagtcatg atggacgacc
    16021 tggcgcaggt ctggcgcggt gaaacgctgg cgccgctcgc tattaccttc cgtgattatg
    16081 tgatggctga acaggcgcgc cgacagacat cggcatggca cgatgcctgg gattactggc
    16141 aggaaaaact gccgcaactg cccttagcgc cagagctgcc ggtggttgag acgcccccgg
    16201 aaacgccaca cttcaccacc ttcaaatcga cgatcggcaa gacagaatgg caggccgtga
    16261 aacagcgctg gcagcagcaa ggcgtcacac cgtctgccgc gctgctcacg ctgtttgccg
    16321 ccacccttga gcgctggagc cgtaccacaa catttacgct gaacctgacg ttcttcaatc
    16381 gccagccgat ccatccgcaa atcaaccagt tgattggtga ttttacctcc gtcacgctgg
    16441 ttgattttaa cttctcagcg ccggtgacgt tgcaagagca gatgcaacag acccaacagc
    16501 gcctctggca aaacatggcg cacagtgaaa tgaacggtgt tgaggtgatc cgtgagctgg
    16561 gccgcctgcg cggatcacaa cgtcaaccgc tgatgccggt agtgtttacc agtatgctgg
    16621 ggatgacgct ggaaggcatg actatcgatc aggcgatgag ccatctgttc ggcgaaccct
    16681 gctatgtatt cacgcaaacg ccgcaggtct ggctggatca tcaggtcatg gagagcgacg
    16741 gcgagttgat gtttagctgg tactgcatgg acaacgtgct ggaacccggc gctgccgagg
    16801 cgatgtttaa tgactattgc gccatcctgc aagccgtcat cgccgcccct gaaagcctga
    16861 agactctcgc cagcggcatc gccaggcaca ttccccgccg acgctggccg ctgaacgcgc
    16921 aggcggacta cgacctgcgg gatattgagc aggcgacgct cgaatacccc ggcatccggc
    16981 aggccagagc ggaaataacc gaacagggcg cgttgacgct ggatatcgtg atggccgacg
    17041 atccttcgcc atcagcggcg atgcctgatg agcacgaact tacccaactg gcgctgccgt
    17101 tgcctgagca ggcgcagctt gatgagctgg aggcgacctg gcgctggctg gaggcgcgtg
    17161 cgctacaggg gatcgcggct acgctaaatc gtcacggcct gtttaccacg ccggagatcg
    17221 cccatcgctt tagcgcagta gtacaggcgc tgtccgcgca agcgtctcac cagcgtctgc
    17281 tgcgccagtg gctacagtgt ctgacggaaa gagagtggtt aatccgcgaa ggtgaaagct
    17341 ggcgctgccg cattccgctc agcgagattc ctgagcctca ggaagcgtgc ccgcaaagcc
    17401 aatggagcca ggcgctggcg cagtatctgg aaacctgcat cgcccggcac gacgccctct
    17461 tctccgggca gtgttctccg ctggaattgc tgttcaacga gcagcatcgc gttaccgacg
    17521 cgctgtatcg cgacaacccc gccagcgcct gtctgaatcg ctataccgcg cagattgccg
    17581 ccttgtgcag cgcagaacgg attctggagg ttggcgccgg aaccgcagcc actaccgcgc
    17641 cggtgctgaa ggccacgcgg aacacgcggc agtcgtacca cttcacggac gtctccgcgc
    17701 agttcctcaa tgacgccaga gcccgtttcc atgatgaatc gcaggtgtct tatgccttgt
    17761 tcgacatcaa ccagccgctg gatttcaccg cccacccgga ggcgggttac gacctgatcg
    17821 ttgccgtcaa tgtgctccac gacgccagcc atgtcgtcca gacgttgcgc agattaaaac
    17881 tgttgctgaa agccggcgga cgtttgctga tcgttgaagc gacggagcga aacagcgtat
    17941 tccagctggc gagcgtgggc tttattgagg gattaagcgg ataccgcgat ttccgccgcc
    18001 gggatgagaa accgatgctc acccgctccg catggcagga ggttctcgtt caggccgggt
    18061 ttgcaaacga gctggcgtgg cccgcgcagg aatcgtcgcc gctgcgccag catctgctgg
    18121 tagcgcgttc gcctggcgta aatcgcccgg ataaaaaagc cgtgagccgc tatttacagc
    18181 agcgctttgg caccggtctg cccattttac agatccggca aagagaagcg ttatttacgc
    18241 cgctgcatgc cccgtctgat gcgccgactg agccagccaa acccacgcca gttgccgggg
    18301 ggaatccggc actggaaaaa caggtggctg aactctggca atcgctgctg tctcgccccg
    18361 tggcaaggca tcacgacttt ttcgaactgg gcggcgacag cctgatggcg acaaggatgg
    18421 tcgcgcagct aaaccggaga gggattgcca gggctaacct tcaggatctg ttcagccatt
    18481 cgacgctgag cgacttctgc gcccatctac aggcggctac gtcaggagag gacaacccga
    18541 tacccctttg ccagggcgac ggtgaggaaa ccctgtttgt cttccacgct tcagacggcg
    18601 atatcagcgc ctggctgccg ctcgctagcg cgttgaacag gcgcgttttc ggcctgcaag
    18661 caaaatcgcc gcagcgcttt gccacgctcg accagatgat cgatgagtat gtcgggtgca
    18721 tccgtcgtca gcagcctcac ggcccttatg tgctggcggg ttggtcgtat ggcgcgtttc
    18781 ttgcggcggg cgccgcacaa cgcctgtacg ccaaaggcga gcaggttcgg atggtgttaa
    18841 tcgatcccgt gtgccgacag gatttctgtt gcgaaaaccg ggcggccctg ctgcgcctgt
    18901 tagccgaagg acaaacgcct ctggcactgc ccgaacattt cgaccagcag acgcccgaca
    18961 gccagcttgc cgactttatc agcctcgcta aaacggccgg tatggtgtcg caaaacctga
    19021 cgctgcaagc ggcagaaacg tggctcgaca acatcgcgca tctgctgcgt ttactgactg
    19081 agcatacgcc gggcgaaagc gttccggtcc cctgtctcat ggtgtatgcc gccgggagac
    19141 ccgcgcactg gacgccagca gaaaccgagt ggcagggctg gataaacaac gccgacgacg
    19201 ctgtgattga agccagccac tggcaaatca tgatggaagc cccccacgtt caggcttgtg
    19261 cgcaacacat tacgcgctgg ctttgcgcaa cctcaacgca accggagaac acgttatgat
    19321 gccgtccgcc tccccaaaac aacgcgtact gattgtgggc gccaaatttg gcgaaatgta
    19381 cctgaatgcc tttatgcagc ccccggaggg gctggaactg gtcggcctgc tggcgcaggg
    19441 aagcgcccgt tcaagagagc tggctcatgc gtttggcatt ccgctgtata cctcgccgga
    19501 acagataacc aggatgccgg atatcgcctg tattgtcgtg cgttcgaccg tcgccggagg
    19561 aaccggtacg cagcttgcca gacactttct gacgcgcggc gtacacgtca ttcaggagca
    19621 tcccctccac ccggatgaca tcagttcctt acaaacgctg gcgcaggaac agggctgctg
    19681 ctattgggta aacacgtttt atccgcatac gcgcgcggga cgcacatggc ttcgcgatgc
    19741 gcagcagctg cgtcgctgtc tggctaagac gccgcaggtg gtccacgcca ccaccagccg
    19801 acagttgctc tactccactc tggatttgtt gttgctggct ctgggcgttg atgccgccgc
    19861 cgtggagtgc gacgtggttg gcagctttag cgattttcac tgtctgagac tcttctggcc
    19921 ggagggagaa gcctgcctgc tgcttcagcg ctatctcgat cctgacgatc cagatatgca
    19981 tagccttatc atgcaccgcc tgctgctggg ctggccggaa ggtcatcttt cgctggaggc
    20041 gagctacggc cccgtcatct ggtcatccag cctgtttgtc gcagaccatc aggagaacgc
    20101 ccacagcctc taccgcagac cggagatcct gcgcgatctg ccgggtctga cgcgcagcgc
    20161 ggcgcccctg agctggcgcg actgctgcga aaccgtcggg ccggagggcg tcagctggtt
    20221 gctacaccag ttgcgcagcc atctggcggg cgaacatcca ccagcggcct gccaaagcgt
    20281 acatcagatc gcgttatcac gtttatggca gcagatttta cgtaagaccg gcaatgcgga
    20341 gatcagacgc ctgacgccgc cgcaccacga ccggttagcc ggtttctaca acgatgatga
    20401 taaggaggcg ctgtgacgca atctgcaatg tgcatcccgc tgtggcccgc ccggaacggc
    20461 aatactgcgc atctggtcat gtgccctttc gctggcggca gcagtagcgc gtttcgccac
    20521 tggcaagctg agcaactggc tgattgcgcg ctttctctgg tgacctggcc ggggcgcgat
    20581 cgccttcgcc atctggaacc gctcagaaac attacacaac tggcggcact gctggcgaac
    20641 gagctggaag catccgtatc gcctgacacg ccgcttttac tcgccgggca cagcatgggg
    20701 gcgcaggtgg cgtttgaaac ctgccgactt ctggagcaac gggggcttgc gccacaagga
    20761 ctgattattt ccgggtgcca tgccccgcat ctgcattctg aacgccagct cagccatcgc
    20821 gatgatgccg actttatcgc tgagctgata gacattggcg gatgttctcc tgaactgcgg
    20881 gaaaaccagg aattaatgtc gctgtttctt cctcttctgc gcgctgattt ttacgccacc
    20941 gagagctatc actacgactc gcccgacgtc tgtccgccgc tgcgcacgcc tgcgctgtta
    21001 ttgtgcggca gccacgatcg cgaagcctcc tggcagcagg tcgatgcctg gcgtcagtgg
    21061 ctgagccacg ttacaggccc ggtggtgatt gacggcgatc atttctatcc cattcaacaa
    21121 gcccggtcct tttttacgca gattgtccgc cattttcccc acgcattttc tgcaatgacc
    21181 gcgtggcaaa aacagcccag tacttcagaa aggtgacgca tgaattcttc ctttgaatct
    21241 ctgattgaac agtatccctt acccattgcc gaacagttgc gccactgggc ggcccgttat
    21301 gcctcgcgaa ttgccgtcgt tgatgcaaag gggtcgttaa cctacagcgc gcttgatgca
    21361 caagttgacg aacttgccgc aggtctgtca tcactgggtt tgcgttcggg ggagcatgta
    21421 attgtgcagc ttcccaacga caacgcgttt gttaccctgc tgttcgcctt gttaagactg
    21481 ggcgttatcc ccgtgctggc gatgccctcg caacgggctc tggatatcga cgcgctgatt
    21541 gagctggcgc aacccgtcgc ttacgttatt cacggggaaa acctcgcaga gctggcccga
    21601 cagatggcgc acaaacacgc ctgcttgcgt catgttctgg tcgctggaga gaccgtgagc
    21661 gacgatttta cgccgctctt ctcccttcac ggtgagcgac aggcatggcc gcagcctgat
    21721 gtttccgcca ccgcgttgtt gttgctctca ggcggcacaa ccggcacgcc caaactcatc
    21781 ccgcgccgac atgccgacta tagctataac ttcagcgctt ctgctgaact gtgcggcatc
    21841 agccaacaga gcgtgtatct cgccgtcctc ccggtggcgc ataactttcc gctggcctgc
    21901 cccggtattc tgggaacgct tgcctgcggc ggaaaagtgg tgctgaccga cagcgccagc
    21961 tgtgatgagg tgatgccttt aatcgcgcag gaaagagtga ctcacgtcgc cctggttccg
    22021 gcgctggcgc aattatgggt gcaggccagg gagtgggaag acagcgacct ttcgtcgctg
    22081 cgcgtcattc aggcaggcgg cgcccggctc gacccgacgc ttgctgagca ggttatcgcc
    22141 acctttgact gtaccctgca acaggttttc ggtatggcgg aaggcctgct ctgttttacc
    22201 cggctggacg atccgcatgc caccattctc cacagccagg ggcgcccgtt gtcccctctg
    22261 gatgaaatcc gcatcgttga tcaagacgag aacgacgtcg cgccgggcga aaccgggcaa
    22321 ttgttaacgc gcggccctta taccatttcg ggctattacc gcgcccctgc ccacaacgcg
    22381 caggccttta ccgcgcaagg gttttaccgc acaggcgaca atgtcaggct ggatgaggtg
    22441 gggaacctgc acgttgaggg acgcataaaa gagcagatca accgcgccgg agaaaaaata
    22501 gccgcggctg aagtggaatc ggcactgctg cgtttagcgg aagtgcaaga ttgcgcggtg
    22561 gtcgccgcgc cggacacgct gcttggcgag cggatttgcg cgtttatcat cgcgcagcag
    22621 gtgccaactg actatcagca gttgcgtcaa caactgaccc gtatggggct cagcgcgtgg
    22681 aaaattcctg accaaatcga gtttctggac cactggccgc tcaccgccgt cggcaagata
    22741 gacaaaaaac gcctgacggc tctcgccgtc gaccgttatc gccattctgc ccaataagcg
    22801 caaaccgacc cgaaacaggt tgaaataaac ccgtttcggg tagcaccact attagaaata
    22861 gttatcattt tcaattcacc attgtcggta tttttggcgt ttcgccgtct tacagggact
    22921 cacaacaatg aaaatgacac ggctttatcc tctggccttg gggggattat tgctccccgc
    22981 cattgctaat gcccagactt cacagcaaga cgaaagcacg ctggtggtta ccgccagtaa
    23041 acaatcttcc cgctcggcat cagccaacaa cgtctcatct actgttgtca gcgcgccgga
    23101 attaagcgac gccggcgtca ccgccagcga caaactcccc agagtcttgc ccgggctcaa
    23161 tattgaaaat agcggcaaca tgcttttttc gacgatctcg ctacgcggcg tctcttcagc
    23221 gcaggacttc tataaccccg ccgtcaccct gtatgtcgat ggcgtccctc agctttccac
    23281 caacaccatc caggcgctta ccgatgtgca aagcgtggag ttgctgcgag gcccacaggg
    23341 aacgttatat ggcaaaagcg ctcagggcgg gatcatcaac atcgtcaccc agcagccgga
    23401 cagcacgccg cgcggctata ttgaaggcgg cgtcagtagc cgcgacagtt atcgaagtaa
    23461 gttcaacctg agcggcccca ttcaggatgg cctgctgtac ggcagcgtca ccctgttacg
    23521 ccaagttgat gacggcgaca tgattaaccc cgcgacggga agcgatgact taggcggcac
    23581 ccgcgccagc atagggaatg tgaaactgcg tctggcgccg gacgatcagc cctgggaaat
    23641 gggctttgcc gcctcacgcg aatgtacccg cgccacccag gacgcctatg tgggatggaa
    23701 tgatattaag ggccgtaagc tgtcgatcag cgatggttca ccagacccgt acatgcggcg
    23761 ctgcactgac agccagaccc tgagtgggaa atacaccacc gatgactggg ttttcaacct
    23821 gatcagcgcc tggcagcagc agcattattc gcgcaccttc ccttccggtt cgttaatcgt
    23881 caatatgcct cagcgctgga atcaggatgt gcaggagctg cgcgctgcaa ccctgggcga
    23941 tgcgcgtacc gttgatatgg tgtttgggct gtaccggcag aacacccgcg agaagttaaa
    24001 ttcagcctac gacatgccga caatgcctta tttaagcagt accggctata ccaccgctga
    24061 aacgctggcc gcatacagtg acctgacctg gcatttaacc gatcgttttg atatcggcgg
    24121 cggcgtgcgc ttctcgcatg ataaatccag tacacaatat cacggcagca tgctcggcaa
    24181 cccgtttggc gaccagggta agagcaatga cgatcaggtg ctcgggcagc tatccgcagg
    24241 ctatatgctg accgatgact ggagagtgta tacccgtgta gcccagggat ataaaccttc
    24301 cgggtacaac atcgtgccta ctgcgggtct tgatgccaaa ccgttcgtcg ccgagaaatc
    24361 catcaactat gaacttggca cccgctacga aaccgctgac gtcacgctgc aagccgcgac
    24421 gttttatacc cacaccaaag acatgcagct ttactctggc ccggtcggga tgcagacatt
    24481 aagcaatgcg ggtaaagccg acgccaccgg cgttgagctt gaagcgaagt ggcggtttgc
    24541 gccaggctgg tcatgggata tcaatggcaa cgtgatccgt tccgaattca ccaatgacag
    24601 tgagttgtat cacggtaacc gggtgccgtt cgtaccacgt tatggcgcgg gaagcagcgt
    24661 gaacggcgtg attgatacgc gctatggcgc actgatgccc cgactggcgg ttaatctggt
    24721 cgggccgcat tatttcgatg gcgacaacca gttgcggcaa ggcacctatg ccaccctgga
    24781 cagcagcctg ggctggcagg cgactgaacg gatgaacatt tccgtctatg tcgataacct
    24841 gttcgaccgt cgttaccgta cctatggcta catgaacggc agcagcgccg tcgcgcaggt
    24901 caatatgggt cgcaccgtcg gtatcaatac gcgaattgat ttcttctgat tattgtaaaa
    24961 gggataccga aaaggtatcc cttttacacc actagttaaa accagtaact cagcagagtc
    25021 gcaaaaatat taatccatag tgattattta aacaatgaaa ttgcgattag gataaataga
    25081 tttaactttc tcgttccttt ctctccttat actaaagaaa taatcatatc aaaataaaaa
    25141 ttcacaacag tgcaacatta aaaaatacaa ccaacaaaca atcctatata caaggcacat
    25201 ctccagaata taaaagcaca gacaaacaac ctaaaaaaac aaccccatac atataaatta
    25261 ttttataggt aaaatagatt atatattctc atagctacca taaactatac tagcctgaac
    25321 tatatttatt ctgctacaat caatgcatac ataacacaaa tatcactcag gtacactact
    25381 caaaccacgc tgggattttt cctcaagtta taatcatcac cccgaaaatc attcggcatt
    25441 tactcattaa atagtcaccc cataggcctg tacatgttta ctcagaaata tacatccttt
    25501 tctctgtcat aaaccctctg attaatcata aataaatact tgtgacacca atctttttcc
    25561 ttaacggaac gaattgttgt gtagaaggag ataatattat gtcaaaaaaa tatcagccat
    25621 tgcttataac tcattatatg tcaacatggg tcactataac ggaagcagtt gagatcacta
    25681 ccaaagccat aaaacaaaaa attactccta gcgatattta tcgtcatgcc ttgagtggca
    25741 acatcctact atcgatctat ttccagtctc ctgtgatact taagaagata caaactttta
    25801 atggaaaaat aaaattcagg cagtttgtgg gagacctact tgataaacta tgcatgcttg
    25861 acagtgacgg ttttatttat ggtcaaaatt taagactctg tacagaagcc agatatattt
    25921 gtcctgtgca acaaattata gatacccccc tgttacgcaa actcaaccag tttcgtacgt
    25981 ttgttcgtaa tgtccgcccg ggtgatgaac tggatgtcca ggcacaagtt agtgaaaaaa
    26041 atttaacccc gccgccgggt aacagcagtg gcaaccttga gcaacagata gccagtactt
    26101 cacagctaat tggatctctg ctcgccgaag atatgaacag cgagcaagcg gcaaacatag
    26161 cacgtggatg ggcctcttct caggcttcag gggtaatgac agactggtta agccgcttcg
    26221 gtaccgcaag aatcacgctg ggcgtggatg aagatttcag cctgaagaac tcccgtgacg
    26281 gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga actatgtgcc gaacgtcacg
    26341 aatgctgaaa tcacgctggc agcctcgaag gatccggtga ttgccgacaa taacgatctc
    26401 acgacactaa cagcaccgtc gctgatacag agggcaatgc gatagccaac actgaggtaa
    26461 catttactct accggaagat gtgaaggcga acttcacgct gagcgatggc gacaaagcgg
    26521 ttactgatgc cgatggcaaa gcgaaagtca cgctgaaagg tacaaaagca ggcgctcata
    26581 ccgtgacagc atcgatggtt ggcggtaaga gtgagcagtt ggtggtgaac tttactgcgg
    26641 atacgctcac tgcgcaggtt aatcttaacg ttaccgagga caatttcatc gccaataaca
    26701 ttggaatgac caggctacaa gcaacagtga ctgatggaaa cggcaaccca gtagaaggta
    26761 taaaagttaa tttccgtgga acctccgtca cgctaagcag caccagcgtt gaaacggatg
    26821 atcaggtttt tgctgaaatt cttgtgacaa gcaccgaggt cggactaaaa acagtttcag
    26881 cctctctggc agataaacct actgaagtca tctcgcgatt actgaatgcc aaggtagatg
    26941 ttaattctgc gacgattacc agtcaggaga tacctgaagg tcaggtcatg gtcgcacaag
    27001 acatagcagt taaagctcac gttaacgacc agtttggcaa cccggtgacg catcaacccg
    27061 cgacattcag tgcagcgcca tcctcgcaaa tgatcatcag ccagaacacg gtctctacta
    27121 atacgcaggg agtagccgag gtcaccatga cgtctgaaag aaacggctcg tatactgtaa
    27181 aagcatccct agcgaatgga gcttcacttg agaaacaact ggaggctatt gatgaaaaac
    27241 tgacactcac gtccagtccg cttatcggtg tcaatgctcc taaaggcgct actctgacgg
    27301 caacgctaac ctctgcaaat ggcactccag tggagggtca ggtcatcaac tttagcgtaa
    27361 cgctagaagg tgcgacgtta agtggcggaa aagtgagaac caactcttca gatcaggctc
    27421 cagtcgttct gaccagcaat aaagtcggta catatacggt gactgcatcg ttccataacg
    27481 gcgtcacaat acagacacag acaaccgtga aggttactgg caacccaagc accgcacatg
    27541 ttgctagctt tatcgctgat ccgtcgacta tcgccgccac caacagtgat ttaagtacct
    27601 taaaggcaac ggttgaggat ggcagtggta acctgatcga aggtctcact gtgtacttcg
    27661 ccttaaaaag cggctccacc acattaacgt cattaacagc cgtgaccgat caaaacggaa
    27721 tcgcgacaac aaacgtgaga tgagcgataa cagggagcgt cactgtaagc gcagtcataa
    27781 gcgcaggtgg aatgcaaaca gtagatatat cgctagtggc aggcccagca gacgcctcgc
    27841 agtccatcct taagaacaac cagtcatcac tgaaagggga ctttaccgat agtgctgagc
    27901 tacatcttgt tctgcacgat atatcaggca atccgatcaa agtttcttaa gggatagaat
    27961 ttgtgcaatc aggtacaaac gtgccctata tgaaaattag cgcaattgat tacagtcaaa
    28021 atatcaacgg tgattacaaa gctactgtta caggaggcgg agagggtatc gcaacgctga
    28081 tccccgtatt gaatggtgtt catcaggcgg gtctgagtac cacgatagaa ttcattagcg
    28141 cggaaaccag accaatgacg ggtacagtgt cagtgaatag tgcaaaccta ccgacagctt
    28201 cattcccttc gcagggattt accggggcgt attatcagtt gaataatgac aactttgcac
    28261 caggaaaaac ggcggctgat tattcgtttt caagctcggc ctcctgggta ggcgttgatg
    28321 ctactggtaa agtgacattc aaaaatgacg gtgatagcaa tactgtaata atcacagcgc
    28381 ccccaagatc cggtggtgct atttaccaaa cagttcctcc tgaatcgcgt tcggtgtgac
    28441 tatgatcatt ttcggtgagt attgctttga tccaaaggct ggttttctgt gatgattgac
    28501 cagcctcaga gtattgatga caccaccgtt cataacagat cggcattact atgcataagt
    28561 actgcctcaa attctcgcca aaacactttt taatgccttt gcgaattcat ccagacttat
    28621 attgtcaatt ttttgcccaa cgacgactga ctccgaaaca tcggccattg cgggccatcg
    28681 cgttttgtaa tcagcataaa ttgcaacaac aggggtatta taggcacttg ccatatgtaa
    28741 tattgaggtg tcaggagtta ttgcaatata agcatctttt atgattgctg ctgaacgttt
    28801 aatcgagggt gataaggata aacggtagac attattataa caatccacta atgccctggc
    28861 tttatcttcc cctttgggtc cataaacgat cactattgga atatctgttt cactttgaat
    28921 ttttgctatt agattttctg caatcgataa tgaaaatgta cgttcctgcg agctaccttc
    28981 taaattgagc gcaatgtaag atcctaacga tcgcatttcc tcgcgtacct catcgagtac
    29041 atcatcgctt aatggcaatt catatattga cctgactgcc ggaaaacccg cctcacgcat
    29101 cataaatgcc catgtcatgg gtacaggagc ccggagattc tgatccatcc gggacgcgtt
    29161 cttacacaaa ggggaatagc acttcatggt taaaccgacg acctggaaat tcgtttttgc
    29221 tttcaactga ctgataaata tcatcgtttt caggttcttt ttacgcatcg cctctataca
    29281 aagatccggc gtaccatatt gcagtgttat gttcttcgct aaatctttta tttcttttaa
    29341 tgttgcgtga tcctgcatag tcattgtgac taatgttaat ttagtctgtt caagtttgag
    29401 cgcattaaag acttctaaat taattgtcga cgtaacaatt aatagatgct taattttatg
    29461 caattcaagc gcccgaataa caggaaagat ggccatagca tcgccaatct gatcgggaat
    29521 atgtatgaca acaaagtctg ttttttcaat attaaaatta taagttttat aatcgtagta
    29581 actaaatgca atacgtctca acaatgatac taaaaacata actaacctcg cctccctact
    29641 ggttataatg caatgcagtc tatcagaatc atcagggtgc aattttgtgc atatgcggac
    29701 ttttatgttt catatctcga acctgtgggc cctctgctta atccttaaac aacaccagca
    29761 actcctgcgc tttcatcttc catcgaattt ttcatgttgc cgctaatcag ccataaaatc
    29821 atttgcagat gcgctctgtc gaggtagtct cataaggttc gtttatagat cgacggcaat
    29881 gtgagttacc ttttccatac taattataaa aagacagtac aaacaggatc attatggact
    29941 ccacgctcat ctccactcgt cccgatgaag ggacgctttc gttaagtcgc gcccgacgag
    30001 ctgcgttagg cagtttcgct ggtgccgtcg tcgactggta tgattttcta ctctatggca
    30061 ttaccgccgc actggtgttt aatcgcgagt ttttcccgca agtaagcccg gcgatgggaa
    30121 cgctcgccgc atttgccacc tttggtgtcg gcttcctttt ccgaccgctc ggcggtgtca
    30181 ttttcggtca ctttggcgac cgactgggac gtaagcgcat gttaatgctg accgtctgga
    30241 tgatgggcat cgcgacagcc ttaattggta ttcttccttc attctcgacc attgggtggt
    30301 gggcacctat tttgctggtg acactgcgtg ccattcaggg atttgccgtc ggcggcgaat
    30361 ggggaggcgc agcgttgctt tccgttgaaa gtgcgccgaa aaataaaaaa gccttttaca
    30421 gtagcggtgt acaagttggc tacggtgtag gtttactgct ttcaaccgga ctggtttcat
    30481 tgatcagtat gatgacgact gacgaacagt ttttaagctg gggctggcgc attcctttcc
    30541 tgtttagcat cgtactggta ctgggagcat tgtgggtgcg caatggcatg gaggagtccg
    30601 cggaatttga acaacagcaa cataatcaag cggccgcgaa aaaacgcatc ccggttatcg
    30661 aagctctgtt acgacatccc ggtgctttcc tgaagattat tgcactacga ctgtgcgagt
    30721 tgctgacgat gtacattgtt actgcctttg cacttaatta ttcaacccag aatatggggt
    30781 taccgcgcga acttttcctt aatattggtt tgctggtagg tggattaagc tgcctgacaa
    30841 ttccctgttt tgcctggctt gccgatcgtt ttggtcggcg cagggtttat attacaggcg
    30901 cgttgatcgg aacgttgagc gcatttcctt tctttatggc gcttgaagca caatctattt
    30961 tctggatagt tttcttctcc ataatgctgg caaacattgc gcatgacatg gtggtgtgtg
    31021 tgcaacaacc gatgtttacc gaaatgtttg gtgccagtta tcgctacagt ggtgctggag
    31081 tcggttatca ggttgccagt gtggttggcg gtggatttac accttttatt gccgctgcac
    31141 tcatcactta ctttgccggg aactggcata gcgtcgccat ttatttgctg gctggatgcc
    31201 tgatttctgc aatgaccgct ttattgatga aagacaatca acgctcgtga tagcctggcg
    31261 aagatcatcc gatcttcgcc ttatactttt gtttcacatt cctgtgacat actatcggat
    31321 gtgcggtaat tgtatggaac aggagacaca catgaataat aagggctccg gtctgacccc
    31381 agctcaggca ctggataaac tcgacgcgct gtatgagcaa tctgtagttg ctttacgcaa
    31441 cgccattggc aaatatatta caagtggcga attacctgat gaaaacgccc gcaaacaagg
    31501 tctttttgtc tatccatcac tgaccgtaac ctgggacggt agcacaacta atccccccaa
    31561 aacgcgcgca tttggtcgtt ttacccacgc aggcagctac accaccacga ttactcgccc
    31621 tactctcttt cgttcgtatc tcaatgaaca acttacgttg ctgtatcagg attatggtgc
    31681 gcatatctcg gtgcaaccct cgcagcatga aatcccttat ccttatgtca tcgatggctc
    31741 tgaattgaca cttgatcgct caatgagcgc tgggttaact cgctacttcc cgacaacaga
    31801 actggcgcaa attggcgatg aaactgcaga cggcatttat catccaacgg aattctcccc
    31861 gctatcgcat tttgatgctc gccgggttga tttttccctc gcacggttgc gccattatac
    31921 cggtacgcca gtagaacatt ttcaaccgtt cgtcttgttt accaactaca cacgttatgt
    31981 ggatgaattc gttcgttggg gatgcagcca gatcctcgat cctgatagtc cctacattgc
    32041 cctttcttgt gctggcggga actggatcac tgccgaaacc gaagcgccag aagaagccat
    32101 ttccgacctt gcatggaaaa aacaccagat gccagcatgg catttaatta ccgctgatgg
    32161 tcagggtatt actctggtga atattggcgt gggaccgtcc aatgctaaaa ccatctgcga
    32221 tcatctggca gtgctacgcc cggatgtctg gttgatgatt ggtcactgtg gcggattacg
    32281 tgaaagccag gccattggag attatgtcct tgcacacgct tatttacgcg atgaccacgt
    32341 tcttgatgcg gttctgccgc ccgatattcc tattccgagc attgctgaag tgcaacgtgc
    32401 gctttatgac gctaccaagt tagtcagcgg caggcctggt gaagaagtca aacagcggtt
    32461 acgtactggc acggtggtaa ccacagatga caggaactgg gagttacgtt actcagcttc
    32521 tgcacttcgt tttaacttaa gccgggccgt agcaattgat atggaaagtg caaccattgc
    32581 cgcgcaagga tatcgtttcc gcgtgccata cgggacacta ctgtgtgttt cagataaacc
    32641 gttacatggt gagattaagc ttcccggcca ggctaaccgt ttttatgaag gcgctatttc
    32701 cgaacacctg caaattggca ttcgggcgat cgatttgctg cgcgcagaag gcgaccgact
    32761 gcattcacgt aaattacgaa cctttaatga gccgccgttc cgataataag ttatgaacct
    32821 tcttatcaga aatagatgtg aaggattaag taagacatct ggcaaatagc ctgcaataaa
    32881 cgcgggctgt ttgcttcctt gggcagatgc gagttttatt atcgtcttaa tgatttccac
    32941 atattaaaag caagtatgct ttcaaaacac aattataaaa aatcccgcca acaacattag
    33001 tttttataaa attaaatata agattatggc tttagaatat ttttatttct aatagacgag
    33061 atttttcctg ttatgatata atatgccgaa ttaacacatg ttaacgattt accagtaatg
    33121 taaataaatt ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa
    33181 aaaaacggct aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta
    33241 tgctgctgcc aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat
    33301 tgaacgtgca aaacaagcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc
    33361 caaaggcggc ggagatgaaa cgttcgtgca gggacgttat gaaggctttg gtcctaatgg
    33421 ctcaatgatt atcgctgaga cattaacttc aaatgttaac cgtacgattg ctaacgttcg
    33481 cacaattttc aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt
    33541 tgacaatacg ggtgtgattg tatttaaagg aacagaccct gaccatattt ttgaaatttt
    33601 gcttgaagct gaagttgatg ttcgtgatgt aactgaagaa gaaggtaata ttgttattta
    33661 tactgaacct actgaccttc ataaaggaat cgcggcgcta aaagcagctg gaatcactga
    33721 gttctcaaca acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga
    33781 tttagaaatc tttgaagggc ttgttgatgc ccttgaagat gacgacgatg tacaaaaagt
    33841 ttatcataac gtcg
//
LOCUS       DAOFSV010000039        32806 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.039, whole genome shotgun sequence.
ACCESSION   DAOFSV010000039 DAOFSV010000000
VERSION     DAOFSV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.039"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(142..1821)
                     /gene="bcsG"
                     /locus_tag="QBH27_001976"
     CDS             complement(142..1821)
                     /gene="bcsG"
                     /locus_tag="QBH27_001976"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDQ7014288.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPHYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPIVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1818..2009)
                     /gene="bcsF"
                     /locus_tag="QBH27_001977"
     CDS             complement(1818..2009)
                     /gene="bcsF"
                     /locus_tag="QBH27_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDQ7014289.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2006..3577)
                     /gene="bcsE"
                     /locus_tag="QBH27_001978"
     CDS             complement(2006..3577)
                     /gene="bcsE"
                     /locus_tag="QBH27_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDQ7014290.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3850..4038
                     /locus_tag="QBH27_001979"
     CDS             3850..4038
                     /locus_tag="QBH27_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDQ7014291.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4050..4802
                     /gene="bcsQ"
                     /locus_tag="QBH27_001980"
     CDS             4050..4802
                     /gene="bcsQ"
                     /locus_tag="QBH27_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDQ7014292.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDASPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4799..7417
                     /gene="bcsA"
                     /locus_tag="QBH27_001981"
     CDS             4799..7417
                     /gene="bcsA"
                     /locus_tag="QBH27_001981"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDQ7014293.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGTRRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYIYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7428..9767
                     /gene="bcsB"
                     /locus_tag="QBH27_001982"
     CDS             7428..9767
                     /gene="bcsB"
                     /locus_tag="QBH27_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDQ7014294.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9774..10880
                     /gene="bcsZ"
                     /locus_tag="QBH27_001983"
     CDS             9774..10880
                     /gene="bcsZ"
                     /locus_tag="QBH27_001983"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDQ7014295.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKENLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10862..14335
                     /gene="bcsC"
                     /locus_tag="QBH27_001984"
     CDS             10862..14335
                     /gene="bcsC"
                     /locus_tag="QBH27_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDQ7014296.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEALLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPMQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14417..16405
                     /gene="hmsP"
                     /locus_tag="QBH27_001985"
     CDS             14417..16405
                     /gene="hmsP"
                     /locus_tag="QBH27_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDQ7014297.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQDLVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16588..17874
                     /gene="dctA"
                     /locus_tag="QBH27_001986"
     CDS             16588..17874
                     /gene="dctA"
                     /locus_tag="QBH27_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDQ7014298.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18094..19590
                     /locus_tag="QBH27_001987"
     CDS             18094..19590
                     /locus_tag="QBH27_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDQ7014299.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19686..20615)
                     /gene="kdgK"
                     /locus_tag="QBH27_001988"
     CDS             complement(19686..20615)
                     /gene="kdgK"
                     /locus_tag="QBH27_001988"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDQ7014300.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSATICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20847..21614
                     /gene="pdeH"
                     /locus_tag="QBH27_001989"
     CDS             20847..21614
                     /gene="pdeH"
                     /locus_tag="QBH27_001989"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDQ7014301.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEELPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21684..23744
                     /locus_tag="QBH27_001990"
     CDS             21684..23744
                     /locus_tag="QBH27_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDQ7014302.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLSFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23923..25245)
                     /locus_tag="QBH27_001991"
     CDS             complement(23923..25245)
                     /locus_tag="QBH27_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDQ7014303.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFTPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25638..26651)
                     /gene="yhjD"
                     /locus_tag="QBH27_001992"
     CDS             complement(25638..26651)
                     /gene="yhjD"
                     /locus_tag="QBH27_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDQ7014304.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGVAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26699..27598)
                     /locus_tag="QBH27_001993"
     CDS             complement(26699..27598)
                     /locus_tag="QBH27_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7014305.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGS
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            28118..28720
                     /locus_tag="QBH27_001994"
     CDS             28118..28720
                     /locus_tag="QBH27_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ7014306.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28771..30420)
                     /locus_tag="QBH27_001995"
     CDS             complement(28771..30420)
                     /locus_tag="QBH27_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDQ7014307.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30825..32222
                     /gene="ccp"
                     /locus_tag="QBH27_001996"
     CDS             30825..32222
                     /gene="ccp"
                     /locus_tag="QBH27_001996"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDQ7014308.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYMPYMQDKQ"
     gene            32433..>32806
                     /locus_tag="QBH27_001997"
     CDS             32433..>32806
                     /locus_tag="QBH27_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ7014309.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT         7887 a         8191 c         8941 g         7787 t
ORIGIN      
        1 tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtaacgtg
       61 tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc ggaccaggcc gcagggggaa
      121 actctgcggc ctttttcgtt cttactgcgg gtaaggcacc cagtcgccgc cgttcaggcg
      181 aacgtacggt ttatcctggt attgaataac tactgcattt gagttctcgg agaccggtgc
      241 tgtttgtggt aacccactgg tgagtttttt ccagtcaaca ttgtcttcgg tgaaaatctt
      301 gccatcaaga acgcgaacca ccagatcgga gatagccagg aagctgctcg gttggtcgat
      361 gacaatcgga gccccctgat gtggtgcctt catgccgaag aatttcaccc caacggggac
      421 gtctgtgata gacgggctag ggatatcacg tagaccagat acctgcattc tgtcgccctt
      481 cagcgcgccg ccgtgttccg gcaccacgac caccatcact ttacgacccg atttctccag
      541 ttcagtaaag aaggcgtcca gttcatcaaa gaatttttgc gcccgcgctt tgtaatccgc
      601 tgttttgctg acgcccggat aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt
      661 ggcactacgg ctgtttttat ctttttcggt aacgtccagc caacggttaa gcacggcggt
      721 atcgtcataa accggcgaac catcaaagcc cagcaaaata accggcagat ttgtttgatc
      781 catcaattcg ctctgcatgc cgccattttc gcgaacttcc ttcaaaaaac caccgaactg
      841 gccgttatgt cccatcatca ggtgctgggt aaagcccagt ttcgacaggt tatcaaacag
      901 atagcagtcg ttatttgccg gttgatacag attagtgtgc gaagtctgcc cgcagctggc
      961 gcgcagtaaa cggatcgccg ccgggccact gtaggaggtg gcggagttaa agttcttgaa
     1021 ctcaatatcg aaatgcgacc acagtggatg cgacatcaat ccggcggctt caatatccga
     1081 ccaggaaagc gaacagatat taatcaccag tagttcaaat ggctgagcat cagcgggcag
     1141 cgaagacggg aagatcgatt tacgtttcgc ctccgcgtta tagaaattat tcagccaggc
     1201 gttaaggttt gctgttgttg gcggtgcagt ttgtgcgggc atatcaccca ctatcggtgc
     1261 gccacccgtc gccgcaacgg ttgccgctgc gttaccaccc gtcgttgtta cagtggtcgt
     1321 tggttgaccg gctggccaca aggagaaact tggtcccgcc agggtaagta cgttcagcca
     1381 tagcagaatg gcaaccacaa aaacggtaat gcgaatccat tgtgacagga ataaccaggc
     1441 cacgaataaa acaaaaatgg ccccaatcat ctgccagtta ataaagcgtg tgacaagatc
     1501 gattaaataa tcggtactga accccgccac ctgcgaaccc tggctcatta tgctttccgg
     1561 accaggcaac caggtgtcat gccagaacaa agcaaagccg atcggcaggg caatccagtg
     1621 gcgcaagcga tgcaggctat agtgcggaat gggcatcagt agaaacgcgg caaacaccaa
     1681 attgaggagc ggatggaagt taagatatcc cgcccacaac aggccgaact taaccagaaa
     1741 atagaagttc cagccggaaa ggccgcgcca gtattgccag agggaagaag gcatggcggt
     1801 attttgcgta aattgagtca ttttttggtt gccctggctt tttccgtgcg gcgtaacgtc
     1861 ccggccggtt taacataacg aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt
     1921 cgcaaagagt gccgcgccag atagcccagc gggaaaaata tcagtgcgca aacgacaata
     1981 atttcaatga tatcgctgat ggtcatcatg atgagcgctc cacagcatca tctaacagtc
     2041 gcattggttc tggtattcgt cgccagtggc gaccatcatg ctcggcattg atgaccggtt
     2101 tagaactttg cgctaaaggc agcggcatgc cccattgttc tggggcaagc aggcgcatct
     2161 gcaccagctc ggcactgatt tgatcatctt caaaccagac cattcggttt gagaaaatgt
     2221 cgccagtagg caatgggaaa atatgattca acgcggtatc cagatcgtta atccgacaga
     2281 atgagagaaa cagcaccagc cgattaccgc caatggtcat gatatcgccg gtacggttag
     2341 ggcgacatag cgtcagggct tgctcaacgc ggatacccgg taccggacgt agggcaacca
     2401 gaacgccttt accgtgggca ggtaatagag ggttattcat catgttgttg acggcattac
     2461 agaacacatc ccacttctgg aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg
     2521 tagtgatatc ttccggcaca tagcgactaa acttctgccc ttgcacgctt tcgatcatcg
     2581 tcagacagcg ggagagtggc gcattccacg ggataaccat atttgcaccg caggccaata
     2641 ataaacgttc atcggtggcg cgcaggctgg cggtattttc ccgtacgagg attttcatcg
     2701 cactaccgcg ctggcgacgc agagtatgaa tgctgcgggc cagtggctcg atttgcgcat
     2761 tttgctgtaa agaaaagacc accgtcgccg cctgagcggt acgggcttca ttgaacagga
     2821 cttcgttatt gttgaacagt tgccagtgtt ccgatagcgg cggcgcacct tccagtacag
     2881 caacattact cagaatgcgt ttttcgtcgc tgcgtggttg gatttccgcc tcttggtgtt
     2941 gaactaatgt ccagcaacca ttttgttgct gaacgctaag ttgctgacgg gcgctgaccc
     3001 ctttttcgtt gcaccagaaa gcaatatcca gcaaatgttg gtcgccctga aaacgcaaac
     3061 tggcaagacc aaaaagtgac cgatattcct caagtaacaa tgaaaattgt ttatcgttat
     3121 tatttccgga attaattacc aaaagcgaac aatggtttag ccggctccat ttattcattt
     3181 tatccaacca tgagcgaagc cgctcggcag gaatgttttg ccatgcatta tttgcgcaaa
     3241 caagaataaa aaggtaatta tggggatcaa tagaacactg caaatcacgg gccaaatagt
     3301 atagaccttt ttcatgattt agcatcgaaa ataatgttat tttttccggc ccttgagaat
     3361 catctaattg aaagattttc gctggatcgc tgtccatgct aatgaccgcg acgtgtgcgg
     3421 tttcagcctg ggatgcaatt gtttgattcg ccagactgat agcatcttca tggcgatcga
     3481 cgttaaacca ccagacgccg cctgctggca tatgtcgcag ctcatcccat aatgatgaga
     3541 taccgataga gaatacaggg tccacaatgt ccctcatgtc aacattttgt ctgattatca
     3601 gtttactagc gaaagctaag aaataaacct accattgaaa ttaaaactta tcagatttag
     3661 catgtgaatc aatttagcag gtcacaagac atgtgatttt agtcttttcg tttttaactt
     3721 tttctgttgc ataacggaaa gtcaaaaagt gagcacattc ccgtctcgcc gtaaacaata
     3781 aaccgacgaa atgctcacag ttagaattgt ctcatgaacg gtacggttat ttcataggga
     3841 tcaagaaaaa tgaataacaa tgaaccagat actctgcctg atcccgcgat aggctatatc
     3901 ttccagaatg atattttggc gttaaagcag gcattttcac tgcctgatat tgattatgcc
     3961 gatatttccc aacgcgaaca gttggccgcg gcattaaaac gctggccgtt gctggcagag
     4021 tttgcgcaac aaaagtaggg gattggtgaa tggccgtact gggattgcag ggggttcggg
     4081 gaggcgtggg gacaacaacc atcaccgccg cattagcctg gtcattacaa atgttgggag
     4141 aaaatgtcct ggtggtcgat gccagcccgg acaacttgtt gcgcctgtca tttaacgttg
     4201 attttaccca ccgtcagggt tgggccagag ctatgctgga tggccaggac tggcgtgacg
     4261 ccgggttgcg ctatacctcg cagctcgatt tgctgccttt tggtcagtta tcgattgaag
     4321 aacaagaaaa tccacagcac tggcaaaccc ggctgagcga tatttgctcc ggcttacagc
     4381 aactaaaagc cagcgggcgt taccagtgga ttttaattga cttaccgcgt gatgcctcgc
     4441 agataaccca ccagctgctg agtttgtgcg atcactcgct ggcaatcgtc aatgtggatg
     4501 ccaactgcca tatccgactg catcagcaag cgctgccgga tggtgcacat attttgatta
     4561 atgacttccg tattggcagc caggttcagg acgatattta ccagctttgg ttgcaaagcc
     4621 agcgccgatt gctgccgatg ctcattcatc gtgatgaagc gatggctgaa tgtctggcag
     4681 ctaagcaacc agtcggtgaa tatcgcagtg atgcgctggc ggctgaagag atactgacgc
     4741 tggcgaactg gtgcctgttg aattattccg ggctgaaaac gccagtcggg agtgcatcat
     4801 gagtatcctg acccggtggt tgcttatccc gccggtcaac gcacggctta ttgggcgtta
     4861 tcgcgattat cgtcgtcacg gcgcgtcggc tttcagcgcg acgctcggct gtttctggat
     4921 gatcctggcc tggattttta ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga
     4981 acataaaaac ctgtatccgc atatcaacgc ctcgcgtccg cgtccgctgg acccggtccg
     5041 ttatcttatt caaacatgct ggttactgat cggtgcatcg cgcaaagaaa cgccgaaacc
     5101 gcgcaggcgg gcattttcag gtctacaaaa tattcgtgga cgttaccatc aatggatgaa
     5161 tgagctgcct gagcgcgtta gccataaaac acagcatctt gatgagaaaa aagagctcgg
     5221 tcatttgagt gccgggacgc ggcggttgat cctcggtatc atcgtcacct tctcgctgat
     5281 tctggcgtta atctgcgtta ctcagccgtt taacccgcta gcgcagttta tcttcctgat
     5341 gctgctgtgg ggggtagcgc tgatcgtacg gcggatgccg gggcgcttct cggcgctgat
     5401 gctgattgtg ctgtcgctga ccgtttcttg ccgttatatc tggtggcgtt acacctcgac
     5461 gttaaactgg gacgatccgg tcagcctggt gtgcgggctt attctgctct tcgctgaaac
     5521 gtacgcgtgg attgtgctgg tgctcggcta tttccaggtc gtatggccgc tgaatcgtca
     5581 gccggtgcca ttgccgaaag atatgtcgct gtggccgtcg gtggatatct ttgtcccgac
     5641 ttacaacgaa gatctcaacg tggtgaaaaa taccatttac gcttcgctgg gtatcgactg
     5701 gccgaaagac aagctgaaca tctggatcct cgatgacggc ggcagggaag agtttcgcca
     5761 gtttgcgcaa aacgtggggg tgaaatatat cgcccgtacc actcatgaac atgcgaaagc
     5821 gggcaacatc aacaatgcgc tgaaatatgc caaaggcgag ttcgtgtcga tattcgactg
     5881 cgaccacgta ccaacgcgat cgttcctgca aatgaccatg ggctggttcc tgaaagagaa
     5941 acagctggcg atgatgcaga cgccgcacca cttcttctcg ccggatccgt ttgaacgcaa
     6001 cctggggcgt tttcgtaaaa cgccgaacga aggcacgctg ttttatggtc tggtgcagga
     6061 cggcaatgat atgtgggatg ccactttctt ctgcggttca tgtgcggtga ttcgccgtaa
     6121 gccgctggat gagattggcg gtattgctgt cgaaacggta accgaagatg cgcacacttc
     6181 tctgcgtctg caccgtcgtg gctacacctc agcgtatatg cgtattccgc aggcagcggg
     6241 gctggcgacc gaaagtctct cggcgcatat cggtcagcgt attcgctggg cgcgcgggat
     6301 ggtgcaaatc ttccgtctcg ataacccgct caccggtaaa gggctgaagt ttgctcagcg
     6361 gctgtgctat gtcaacgcca tgttccactt cttgtcgggc attccacgac tgatcttcct
     6421 gactgcgccg ctggcgttcc tgctgtttca tgcctacatc atctacgcgc cagcgttgat
     6481 gatcgccctg ttcgtgctac cgcatatgat ccatgccagc ctgactaact cgaagatcca
     6541 gggcaaatat cgccactctt tctggagtga aatctacgaa acggtgctgg cgtggtatat
     6601 cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc
     6661 caaaggtgga ctggtggaag aagagtacgt cgactgggtg atctcgcggc cctacatctt
     6721 ccttgttctg ctcaacctgg tgggcgtagc ggttgggatc tggcgctaca tctatggtcc
     6781 gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg gtattctaca acctgattat
     6841 tctcggcggc gcagtcgcag tatcggtaga aagcaaacag gtacgccgat cgcaccgcgt
     6901 ggagatgacg atgcccgcag caattgcccg cgaagatggt cacctcttct cgtgtaccgt
     6961 tcaggatttc tccgacggtg gtttggggat caagatcaac ggtcaggcgc agattctgga
     7021 agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag gaatacgtct tcccggctca
     7081 ggtggcgcgc gtgatgggta atgaagttgg gctgaaatta atgccgctca ctacccagca
     7141 acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat acatgggcgc tctggcagga
     7201 cagctacccg gaagataagc cgctggaaag tctgctggat attctgaagc tcggcttccg
     7261 tggctaccgc catctggcgg aatttgcgcc ttcttcggtg aagggcatat tccgtgtgct
     7321 gacttctctg gtttcctggg ttgtatcgtt tattccgcgc cgcccggagc ggagcgaaac
     7381 ggcacaacca tcggatcagg ctttggctca acaatgatga taacgcgatg aaaagaaaaa
     7441 tattctggat ttgtgcagtg gctctgggga tgagtgcgtt cccctctttc atgacgcagg
     7501 cgacgccagc aacgcaacca ctgatcaatg ctgagccagc tgtagccgcc caggcggagc
     7561 aaaatccgca ggtggggcaa gtgatgccgg gcgtgcaggg cgcagatgcg ccagtcgtgg
     7621 cgcagaacgg tccttcccgt gatgtgaagc tgacctttgc gcaaattgcg ccgccgccgg
     7681 gcagcatggt gctacgtggc attaacccga acggcagcat tgagtttggt atgcgcagcg
     7741 atgaagtggt gacgaaggcg atgctcaacc tcgaatacac gccatcgcca tcattactgc
     7801 ctgtccagtc gcagttaaag gtttatctca atgatgaact gatgggcgtg ctgccagtga
     7861 ccaaagaaca gttgggtaaa aaaacgctgg cacaaatgcc cattaacccg ctgtttatta
     7921 ctgatttcaa ccgtgtgcgg ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc
     7981 cggccagcac cacgctttgg ctggatgttg ggcggagcag tggactggat ctgacctatc
     8041 agaccctgaa tgtgaagaat gacctgtcac acttcccggt gccattcttt gacccgcgtg
     8101 ataaccgcac caacaccttg ccgatggtct ttgcgggtgc gccggatgtt gagctgcaac
     8161 aagcatctgc cattgtcgcc tcgtggtttg gttcgcgttc tggctggcgt gggcagaact
     8221 tcccggtgct ctataaccaa ctgccggatc gcaatgccat tgtctttgcc accaatgaca
     8281 aacgcccgga cttcctgcgc gatcatccgg cggtaaaagc cccggtgatt gagatgatta
     8341 accatccgca gaatccttac gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc
     8401 tgttgcaggc agcgaaaggt atcgctcagg gtaacattct gttccgtggt gaaagtgtgg
     8461 tagtgaatga agtgaaaccg ctgctaccgc gtaaaccgta cgatgcgccg aactgggtgc
     8521 gtaccgatcg tcctgtcacc tttggtgaac tgaaaaccta tgaagaacaa ttacaatcca
     8581 gcggtcttga gccagcagcg attaacgttt cgctaaacct gccgccggat ctctacctga
     8641 tgcgcagtac cggcattgat atggatatta attaccgcta caccatgccg ccggtgaaag
     8701 acagttcgcg gatggatatc agcctgaata accagttcct gcaatccttc aacctgagca
     8761 gcaaacagga ggcgaaccgc ctgctgctgc gtattccggt attacaaggt ttgctggatg
     8821 gcaaaacgga tgtctctatt ccggcgctga aactgggcgc gaccaaccag ttgcggttcg
     8881 actttgagta tatgaacccg atgccgggcg gttcggtgga taactgtatt accttccagc
     8941 cggtgcagaa tcatgtggtg attggtgacg actccaccat cgacttctcg aagtattacc
     9001 acttcatccc gatgccggat ctacgcgcct ttgctaacgc gggcttcccg ttcagccgga
     9061 tggcggattt gtcgcaaacc atcaccgtga tgccgaaaac gccaaacgaa gcacagatgg
     9121 aaaccttgct gaataccgtt ggctttatcg gtgcgcagac gggcttcccg gcgattaacc
     9181 tgacggtgac cgatgatggc agcaccattc agggcaaaga tgccgacatc atgatcgtcg
     9241 gtggtatccc ggacaaactg aaagacgata agcagatcga cctgttggtg caggcgaccg
     9301 aaagctgggt gaaaacgccg atgcgccaga ccccgttccc cggtattgtg ccggacgaga
     9361 gcgatcgcgc ggcagaaacc cagtcaacgc tgacctcttc cggtgcgatg gcggcggtga
     9421 ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat tgcgctgctg gcagatagcc
     9481 cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt
     9541 tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag cctacgtgtt ggcgatgttt
     9601 attacgtagg tcatctgccg tggttcgagc gcttgtggta tgcgctggca aaccatccga
     9661 ttctgctggc ggtgctggcg gctatcagtg tgatattgct ggcgtgggta ctgtggcgtc
     9721 tgctgcgtat tattagtcgt cgtcgtctta acccggataa cgagtaattg aagatgaatg
     9781 tgttgcgtag tggactcgtg acgatgctgc tgctggctgc ctttagtgtt caggcagcct
     9841 gtacctggcc tgcctgggag cagtttaaaa aggattacat cagtcaggaa gggcgcgtca
     9901 tcgaccccag cgacgcgcgc aaaatcacca cctccgaagg gcaaagttac ggcatgttct
     9961 ttgccctggc ggctaacgac cgtgcagctt tcgataatct tctcgactgg acgcagaaca
    10021 atctcgctca gggttcttta aaagaaaatt tgcccgcctg gctgtggggc aagaaagaga
    10081 acagtaagtg ggaagtgctg gacagcaatt cggcctccga tggtgatgtc tggatggcct
    10141 ggtcgttgct ggaggcgggg cgtttgtgga aagagcagcg ttataccgac atcggcagcg
    10201 cattgctaaa acgtatcgcg cgggaggaag tggtgacggt gcctgggctg ggctccatgt
    10261 tgttaccggg caaagtgggt tttgctgagg ataacagctg gcgttttaac cccagctacc
    10321 tgccgccgac gctggcgcag tatttcaccc gctttggcgc gccgtggacc acgctgcgcg
    10381 aaaccaatca acgtttattg ctggaaaccg ccccgaaagg cttttcgcca gactgggtgc
    10441 gctatgagaa agacaaaggc tggcagctaa aagccgaaaa aacgttgatc agcagctacg
    10501 acgctatccg cgtttacatg tgggtaggca tgatgcctga cagcgatccg caaaaagcgc
    10561 ggatgctcaa ccggtttaaa ccgatggcga cattcactga gaaaaacggt tatccgccgg
    10621 aaaaagtgga tgtggctacg gggaaagcgc agggtaaagg accggtcggt ttttctgccg
    10681 ccatgctgcc ctttttacaa aaccgcgatg cgcaggccgt tcagcgccag cgcgtggccg
    10741 ataactttcc cggcagcgat gcctattaca actatgtgct gaccctgttt ggacaaggct
    10801 gggatcaaca ccgtttccgc ttctcgacaa aaggtgagtt attacctgac tggggccagg
    10861 aatgcgcaaa ttcacactaa acatattcac gctttccctc ggtctggccg tcatgccgat
    10921 ggtcgaggca gcaccaaccg ctcagcaaca gttgctggag caagttcggt taggcgaagc
    10981 gacccatcgt gaagacctgg tgcaacagtc gttgtatcgg ctggaactta ttgatccgaa
    11041 taacccggat gtgattgctg cccgttttcg ttctctgtta cgtcagggcg atattgatgg
    11101 cgcacaaaaa cagctcgatc ggctgtcgca gttagcgccg agttcaaatg cgtataaatc
    11161 gtcgcggact acgatgctgc tttccacgcc ggatggtcgt caggcactgc aacaggcaag
    11221 attgcaggca actaccggtc atgcggaaga agctgtggcg agttacaaca aactgttcaa
    11281 cggtgcgccg ccggaaggtg acattgctgt cgagtactgg agtacggtgg cgaaaattcc
    11341 ggctcgccgt ggcgaagcga ttaatcagct aaagcgcatt aatgcggata cgccgggcaa
    11401 cacgggcctg caaaacaatc tggcgctatt gctgtttagt agcgatcgcc gtgacgaagg
    11461 ctttgccgtc ctggaacaaa tggcgaaatc gaacgccggg cgcgaagggg cctctaaaat
    11521 ctggtacggg cagattaaag acatgcccgt cagcgatgcc agcgtgcagg cactgaaaaa
    11581 atatctctcg atctttagcg atggcgatag cgtggctgct gcgcaatcgc aactggcaga
    11641 acagcaaaaa cagctggccg atcctgcttt ccgcgctcgt gcgcaaggtt tagcggcggt
    11701 ggactctggc atggcgggta aagccattcc cgaactacaa caggcggtgc gggcgaaccc
    11761 gaaagacagt gaggctctgg gggcgctggg ccaggcgtat tcccagaaag gtgatcgcgc
    11821 caatgcagtg gcgaatctgg aaaaagccct cgcgctggat ccgcacaaca gcaataacga
    11881 caaatggaac agtctgctga aagtaaaccg ctactggctg gcgatccagc agggcgatgc
    11941 cgcgctgaaa gccaataatc ctgaccgggc agaacggctg ttccagcagg cacgtaatgt
    12001 cgataacacc gacagctatg cagtgcttgg gctgggcgat gtggcgatgg cgcgcaaaga
    12061 ttatcccgcc gccgaacgct attaccagca gaccttgcgt atggacagcg gcaacactaa
    12121 cgccgtgcgc gggctggcga atatttaccg ccagcagtcg ccagaaaaag ccgaagcgta
    12181 tatcgcatcg ctctctgcca gccagcggcg cagcattgat gatatcgaac gcagcctgca
    12241 aaacgaccgt ctggcacagc aggcagaggc actggaaaac caggggaaat gggcgcaggc
    12301 ggcagcactt cagcggcaac gactggcgct ggaccccggc agtgtatgga ttacttaccg
    12361 actttcgcag gatctctggc aagccggaca acgcagccag gccgatacgt taatgcgcaa
    12421 tctggcgcag cagaagccga acgacccgga gcaggtttac gcttacgggc tgtatctctc
    12481 cggtcacgac caggacagag cggcgctggc gcatatcaac agcctgccgc gcgcgcagtg
    12541 gaacagcaat attcaggagc tggttaatcg actgcaaaac gatcaggtgc tggaaaccgc
    12601 taatcgcctg cgagaaaacg gtaaagaggc agaagcggaa gcgctgctgc gccagcaacc
    12661 gccttcctcg cgtattgacc tcacgctggc tgactgggcg cagcagcgac gtgattacac
    12721 cgccgcccgc gcggcttatc agaacgtttt gacgcgcgag cccactaacg ccgatgccat
    12781 tcttggtctg acggaagtgg atattgctgc cggtgacaaa gcggcggcac gtagccagct
    12841 ggcgaaactg cctgccactg ataacgcctc gctgaacaca cagcggcgcg tggcgctggc
    12901 acaggcgcag cttggcgata ccgcagcggc gcagcagaca tttaataagt tgatcccgca
    12961 ggcaaaatct cagccaccgt cgatggaaag cgcgatggta ctgcgtgatg gtgcgaagtt
    13021 tgaagcgcag gcgggcgatc caatgcaggc gctggaaacc tacaaagacg ccatggttgc
    13081 atccggtgtg accacgacgc gtccgcagga taacgacacc tttacccggc tgacccgtaa
    13141 cgacgagaaa gatgactggc tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg
    13201 ccagcaggat cttaacgtca ctctcgagca cgattactgg ggttcgagcg gcaccggtgg
    13261 ttactccgat ctgaaagcgc acaccactat gttgcaggtg gacgcgccct attctgacgg
    13321 gcggatgttc tttcgcagtg atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc
    13381 cgatggcaaa tgggatgaca actggggcac ctgtacatta caggactgta gcggcaaccg
    13441 cagccagtcg gattccggtg ccagcgtggc ggtcggctgg cgaaatgacg tctggagctg
    13501 ggatatcggt accacgccga tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta
    13561 cagcgatgat atcgggccgc tgggttacac cgttaacgcc caccgtcggc ctatctccag
    13621 ttctttgctg gcctttggtg ggcaaaaaga ctctccgagc aataccggga aaaaatgggg
    13681 cggcgtgcgt gccgacggcg tggggctaag tctgagctac gataaaggtg aagcaaacgg
    13741 cgtctgggca tcgcttagcg gtgatcagtt aaccggtaaa aatgtcgaag ataactggcg
    13801 cgtgcgctgg atgacgggct attactataa ggtcatcaac cagaacaatc gccgcgtcac
    13861 aatcggcctg aacaacatga tctggcatta cgacaaagac ctgagtggct actcactcgg
    13921 tcagggcggt tactacagtc cgcaggaata cctgtcgttt gccataccgg tgatgtggcg
    13981 ggagcgcacg gaaaactggt cgtgggagct gggggcgtct ggctcgtggt cgcattcacg
    14041 caccaaaacc atgccgcgtt atccgctgat gaatctgatc ccgaccgact ggcaggaaga
    14101 agctgcgcgg caatccaacg atggcggcag cagtcagggc tttggctaca cggcgcgggc
    14161 attacttgaa cgacgtgtta cttccaactg gtttgtcggc acggcaattg atatccagca
    14221 ggcaaaagat tacgcaccca gccatttcct gctctacgta cgttattccg ccgccggatg
    14281 gcagggtgac atggatttac caccgcagcc gctgatacct tacgccgact ggtaagtttt
    14341 cagatagcgc ctctcttaat gccgctgcga tcgggtatac tcgggcggca atctgggatt
    14401 tccgggggga gacaatttgc gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt
    14461 ggcagccgtt gtcctggtgt tcgtttttat tttttgcacc gttttgctgt tccatctggt
    14521 ccagcagaat cgctataaca cggctacgca actggaaagc attgctcgct ctgtccgcga
    14581 acccttatct tctgccattt tgaaaggcga tattcccgaa gcggaagcta ttcttgccag
    14641 cattaaaccg gcaggcgtgg tcagccgtgc cgatgtagtg ctgcccaacc agttccaggc
    14701 gctgcgtaaa agttttattc cagagcgtcc ggtgccggta atggttactc gcctgtttga
    14761 gctaccggtg caaatctcgc tgggcgttta ctcgctggaa cgtccggcaa acccgcagcc
    14821 aattgcctat ctggtgctac aggcggattc cttccgcatg tataagttcg tgatgagcac
    14881 cctctcaacg ttagtgacca tttacttact tttgtcgtta atattgactg tggcgattag
    14941 ctggtgcatt aaccgcctga ttttgcatcc attacgcaat attgctcgcg aacttaacgc
    15001 catcccaccc caggatcttg ttggtcacca actggcatta ccgcgtctgc atcaggacga
    15061 tgaaatcggt atgttggtgc gcagttacaa cctcaaccag caattgctgc agcgccatta
    15121 tgaagaacag aacgaaaatg cgatgcgctt cccggtgtcg gatttgccga acaaagcctt
    15181 gctgatggag atgctggagc aggtggtggc gcgtaaacaa accaccgcgc tgatgatcat
    15241 cacctgtgaa accctgcgtg atactgcggg tgtgctgaaa gaggcgcaac gagaaattct
    15301 gctgctgacg ctggtggaaa aactcaaatc ggtactgtcg ccacgcatga tcctcgcgca
    15361 gattagcggt tatgactttg ctgtcattgc caacggtgta caggaaccgt ggcacgctat
    15421 cacattaggt cagcaagtgc tcactatcat gagcgagcgc ctgccgattg aacgtattca
    15481 actccgtccg cactgtagca ttggcgtggc gatgttctac ggcgatctca ccgccgaaca
    15541 gctttacagt cgcgctattt ctgcggcatt taccgctcgc cataaaggca aaaatcagat
    15601 tcagttcttt gatccgcagc agatggaagc cgcccagaag cggttgacgg aagagagcga
    15661 tatccttaat gcactggaaa atcatcagtt tgcgatttgg ttacagccac aggtcgagat
    15721 gaccagcggt aaactggtca gtgcggaagt gttactgcgt atccagcaac cggatggcag
    15781 ttgggacctg ccggatggct taatcgatcg cattgagtgc tgtgggctga tggttaccgt
    15841 cggtcactgg gtgctggaag agtcctgtcg attgcttgct gcctggcaag agcgcggcat
    15901 tatgctgccc ttgtcggtaa acctctcagc gctgcaactg atgcatccaa atatggtggc
    15961 ggatatgctg gaactgttaa cccgctatcg cattcagccg ggaacactga ttctggaagt
    16021 gacagaaagc cgacgtattg acgatcctca tgctgcggtg gcaatcctcc gtccgctgcg
    16081 caatgccgga gttcgggtgg cgctggatga tttcggcatg ggctacgcag ggctgcgtca
    16141 gctgcagcat atgaaatcgt tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga
    16201 aggtttgccg gaagatagca gcatgattgc tgcaattatc atgctggcgc agagcctgaa
    16261 cctacaaatg attgccgaag gcgtggagac tgaagcacaa cgcgactggc tggcaaaagc
    16321 gggcgttggt attgcccagg gcttcctttt tgctcgccca ctccctattg aaatcttcga
    16381 agagagttac ctggaagaaa aatagctacc ccaaactgat tacaaaactt taaaaagtgc
    16441 tggtttgtgc gagccagctc aaacttttta acctttttgt ttcaattatg atccaggtac
    16501 atttctgtga tgttgtctgg gtgttatttt aaggccgcag gtaccccata accttacaag
    16561 acctgtggtt ttactaaagg acaccctatg aaaacctctc tgtttaaaag cctttacttt
    16621 caggtcctga cagcgatagc cattggtatt ctccttggcc atttctatcc tgaaataggc
    16681 gagcaaatga aaccgcttgg cgacggcttc gttaagctca ttaagatgat catcgctcct
    16741 gtcatcttct gtaccgtcgt aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt
    16801 cgtaccggcg cagtcgcact gctttacttt gaaattgtca gtaccatcgc gctgattatt
    16861 ggtcttatca tcgttaacgt cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg
    16921 cttgatgcga aagcggtagc ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc
    16981 ttcattatgg atgtcatccc ggcgagcgtc attggcgcat ttgctagcgg taacattctg
    17041 caggtactgc tgtttgccgt actgtttggt tttgcgctcc accgtctggg cagcaaaggc
    17101 caactgattt ttaacgtgat cgaaagtttc tcgcaggtca ttttcggcat catcaatatg
    17161 atcatgcgtc tggcacctat tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac
    17221 ggcgtcggca cactggtgca actgggacag ctgattatct gtttctacat cacctgtatc
    17281 ctgtttgtgg tgctggtatt gggttcaatc gctaaagcga ctggtttcag tatcttcaaa
    17341 tttatccgtt acatccgtga agaactgctg attgtactgg ggacttcatc ttccgagtcg
    17401 gcgctgccgc gtatgctcga caagatggag aaactcggct gccgcaaatc ggtggtgggg
    17461 ctggtcatcc cgacaggcta ctcgtttaat cttgatggca catcgatata cctgacaatg
    17521 gcggcggtgt ttatcgccca ggccactaac agtcagatgg atatcgtcca ccaaatcacg
    17581 ctgttaatcg tgttgctgct ttcttctaaa ggggcggcag gggtaacggg tagtggtttt
    17641 atcgtgctgg cagcgacgct ctctgcggtg ggccatttgc cggtagcggg tctggcgctg
    17701 atcctcggta tcgaccgctt tatgtcagaa gctcgtgcgc tgactaacct ggtcggtaac
    17761 ggcgtagcga ccattgtcgt tgctaagtgg gtgaaagaac tggaccacaa aaaactggac
    17821 gatgtgctga ataatcgtgc gccggatggc aaaacgcacg aattatcctc ttaatctcac
    17881 cacttatgcc cgcactccct tccccgagtg cgggtgtttg cgcataattg acccctgtgc
    17941 ctgaacttac cacctgggtg agttcaggtt tatttcactt cttccgtgac attttcatgt
    18001 tcttgcggtc taacacgaag tgtttttaac gtcatttaga ctgaccgaca acgggcagtt
    18061 ttttattacc aggatagttg atcaggggtt cacatgcagg gcacaaaaat tcgactttta
    18121 gcgggcggtt tgctgatgat ggccactgct ggctatgtgc aggcagatgc gctccagcct
    18181 gatccagcat ggcaacaggg gacgctttcc aacggtttac agtggcaagt gctgactaca
    18241 ccccagcgtc ccagcgatcg tgttgaaatt cgcctgctgg ttaataccgg ttcgctcgcc
    18301 gaaagtacac aacagagcgg ttacagtcac gccatccctc gtattgcgct aacgcaaagc
    18361 ggtggccttg acgcagcgca ggcgcgttca ttgtggcagc aggggatcga ccctaaacgc
    18421 ccgatgccgc cggtaattgt ctcttatgac accacgctgt ttaatctgag tttgcccaat
    18481 aaccgtaacg acttgctgaa agaggcgctc tcttatctgg caaatgccac tggcaaactg
    18541 actatcacgc cagaaaccat caaccatgcg ttgcaaagtc aggacatggt ggcaacctgg
    18601 cctgccgata ctaaagaggg ctggtggcgt tatcgtctga aaggatcaac cttgttaggt
    18661 cacgatcctg ccgatccgct gaaacaaccc gttgaagcgg aaaagattaa agatttctat
    18721 cagaaatggt acaccccgga tgcaatgacg ctgctggtgg tgggaaacgt ggatgcgcgc
    18781 tcggtcgtcg accaaatcaa taaaacgttt ggcgaactga aaggcaaacg tgaaacaccg
    18841 gctccggtgc cgacgctttc tccgctgcgt gcggaagcgg tgagtattat gaccgacgcg
    18901 gtgcgtcagg accggttatc tatcatgtgg gatacgccgt ggcagccgat tcgtgaatca
    18961 gccgcactgc tgcgctactg gcgtgcggac ctggcccgtg aggcgctgtt ctggcatgtt
    19021 cagcaagcgt taagcgccag taacagtaaa gacatcggtc ttggatttga ctgtcgtgtg
    19081 ctgtatctgc gtgcgcagtg tgccatcaac atcgaatcac caaacgacaa gctgaacagc
    19141 aaccttaatc tggtggcgcg tgaactggcg aaggttcgcg ataaaggtct gccggaagaa
    19201 gagttcaatg cgttagtggc gcaaaagaaa ctggagctgc agaagctgtt tgccgcctat
    19261 gcgcgagctg ataccgatat tctgatgggg cagcggatgc gttcgttgca aaatcaggtt
    19321 gtcgatatcg cgccggagca atatcagaaa ctgcgtcagg atttccttaa cagcctgacg
    19381 gtggagatgt taaatcagga tctgcgtcag cagttgtcga atgatatggc gttaatactg
    19441 ctgcagccga aaggcgagcc ggaatttaac atgaaagcgt tgcaggcggc ctgggatcaa
    19501 atcatggccc catcgactgc ggctgccgcc acatctgtcg ccacggatga cgtacatcct
    19561 gaagtgacgg atattccacc tgcacagtaa tgcctgatgg tgatgagtta gcacaaccat
    19621 caggtattta tggatgagct ggataggggg gtaacactct atccagcctt ttgcatatgc
    19681 tgcttttacg ctggcatcgc ttcacgcggg ataatcgcgc cgcgatactg aataacggta
    19741 cttgcggtca ggtgcccgcg tttcgctgcg tcttccgcac tgccgcccgt caaacgtacc
    19801 gccagataac cggcactgaa agagtcgcca gctgcggtgg tatcgatcac tttttctttc
    19861 ggcagtttta ccgccggaac atccactaac ccttcaccag caatggacac caggcaagaa
    19921 tccgccccgc gtttcaccac cacttctttc acgcccgcgt tatgggtgcg cgcaatgacg
    19981 tcttccaccg gctgttgacc ccacagcgcg tcttcatcgt ccagcgtcag gaaggcgata
    20041 tccgtgcatt ccagcatttg ttggtacacc tgctgtgtct cttctttgct ggcccacagg
    20101 cgcggacgat agttattgtc gaaaatcact tttccgccgt tggcgcggca ttcgcgcagc
    20161 agggaaagca gcttttcgcg gctggtcggg cttaagatcg ccaggctaat cccgctcagg
    20221 tagagataat cgaaattcgc cagctcttcg caaatcgtcg cggattgctc actctccagc
    20281 cagaatttgg cggcggcttc gttacgccag tagtagaacg tacgttcgcc ggtgctgtcg
    20341 gtttcaatgt agtaaaggcc cggcagacgg ttttccatcc gttgggtcag ggaagtatca
    20401 acgttctcgc cgcgccaggc gtccagcatc tgctggctaa aactgtccgt tcccagcgcc
    20461 gttacgtaat gaacggttaa tgccgcagga tcgacctgac gggcgatata gacggaagtg
    20521 ttcagggtat cgcccccgaa accgcgctta acgtccgcgc ctttctcgga aagctcaatc
    20581 atgcattcgc caatcacggc aatctttttg gacatagttg tgaacctgat ctgtaaaaaa
    20641 ttagcgttag tttgcgctgt ggtggtttgc tggtcaacca tattaaaaca gtgttccatt
    20701 atttttttga gccagaacgt attcctgaaa gattccgttg tggagtgggg aaatttaccc
    20761 cggccaggcg gtaaagttct gcctttgcgc gccgataatc tttgtcgagt ccgggcagca
    20821 tcacttttaa acacaggaca tctttgatga taaggcaggt tatccagcga ataagcaacc
    20881 ctgaagcaag catcgagagc ttgcaggaac ggcgtttttg gttgcagtgt gagcgtgctt
    20941 acacctggca gccgatctat caaacatgcg ggcggttaat ggccgtggag ctattaacgg
    21001 tggtcacgca tcccttgaac ccttcgcaac gcctgccgcc ggatcgctat tttactgaaa
    21061 tcaccgttag ccatcggatg gaggttgtga aagagcagat tgatttgctg gcgcaaaaag
    21121 ccgacttctt tgtagagcac ggcctgctgg catcggtcaa tattgatggc cctacgctca
    21181 tcgccctgcg tcagcaacca aaaatcctgc gccagattga ggagcttccc tggctgcgtt
    21241 tcgaactggt ggaacacatc cgcctgccga aagattcaac ctttgcctcg atgtgtgaat
    21301 ttggcccgct gtggctggat gattttggta ccgggatggc aaatttctct gcactaagtg
    21361 aagtgcgtta tgactacatc aaaatcgcgc gagaactgtt tgtgatgctg cgtcagtcac
    21421 cggaaggacg cacactcttt tctcagcttt tacatctaat gaatcgctat tgtcgcgggg
    21481 tgattgtcga gggcgtagaa acaccagaag agtggcgtga tgttcagaac tcgcccgcat
    21541 tcgccgcaca aggctggttt ctttcacgcc cggcaccgat agaaacgctg aatacggcgg
    21601 ttctggcgct ataagctgcc tcattttccg cctggctgga ctatctttag gactggcaca
    21661 gaaagaatat gaggaagcga acgatgagca aggcaggcaa aataaccgct gcgatttcag
    21721 gggctttctt gttgttgatt gtcgtggcga tcattttgat tgcaactttt gactggaatc
    21781 gactcaaacc gaccatcaac cagaaagtct ctgcggagtt gaatcgtccg ttcgctatcc
    21841 gtggcgatct gggcgtggtg tgggagcggc aaaaacaaga aactggctgg cgcagctggg
    21901 tgccgtggcc ccatgtacac gcggaagaca tcattcttgg caatccaccg gatattcccg
    21961 aagtcacaat ggtgcatttg ccgcgagtag aagcgacgct ggccccgctg gcgctgctga
    22021 ccaaaacggt ctggttgccg tggatcaagc tcgaaaagcc cgacgcgcgc ctgattcgcc
    22081 tctccgaaaa gaacaataac tggacgttta atctcgccaa cgatgacaac aaagacgcga
    22141 atgcaaagcc gtcggcgtgg tcgtttcggc tggataatat tctgttcgat caagggcgga
    22201 tcgccattga tgacaaagta agcaaagcgg atctggaaat ttttgtcgat ccgttaggca
    22261 agccgctgtc gttcagcgaa gttaccggat cgaaaggtaa agcggataaa gaaaaggtgg
    22321 gcgattacgt ttttggcctg aaggcgcagg gacgttataa cggtgaaccg ctcactggta
    22381 cgggaaaaat aggcggtatg ctggcgctgc gtggcgaagg aacgccgttt ccggtacagg
    22441 ctgatttccg ctctggtaac acccgtgttg cttttgatgg cgttgtgaat gacccaatga
    22501 agatgggggg tgtcgattta cggcttaaat tttctggcga ttcgctgggt gatctctatg
    22561 aactgacggg cgttctgctg cccgatacac cgccgtttga aacggatggt cggctggtag
    22621 cgaaaatcga tactgaaaaa tcgtcggtct ttgattatcg cggttttaat gggcgcattg
    22681 gcgatagcga tatccacggt tctctgatct acaccaccgg caagccacga ccaaaactgg
    22741 aaggtgatgt cgagtcgcgg caattgcggc tggcggacct gggaccgttg attggcgttg
    22801 attccgggaa aggggcagaa aagtcgaaac ggtctgaaca gaagaagggc gaaaaaagcg
    22861 ttcagcctgc gggcaaagtg ctgccttatg accgcttcga aaccgataaa tgggacgtta
    22921 tggatgccga tgttcgcttc aaagggcggc gcattgagca tggcagcagc ctgccgatta
    22981 gcgatctttc tactcatatc atcctcaaaa atgctgacct gcgcctgcaa ccgctgaaat
    23041 ttggcatagc gggcggcagc attgcggcga atattcatct ggaaggcgat aaaaagccga
    23101 tgcaggggcg ggcagatatt caggctcgtc gactgaaact gaaagaactg atgcccgatg
    23161 tagaactgat gcagaagacg ctgggggaaa tgaacggtga cgcggaactg cgcggtagcg
    23221 gtaactcggt ggcggcgctt ttaggcaaca gtaacggcaa cctgaaactg ttgatgaatg
    23281 acgggctggt gagccgcaac ctgatggaga ttgttgggct gaatgtcggc aactacattg
    23341 tcggtgcgat atttggtgat gatgaggtgc gggtgaactg cgcggcggcg aatctggata
    23401 ttgccaacgg cgtggcgcgc ccgcagattt ttgctttcga tactgagaac gcgttgatta
    23461 acgttaccgg tacggcaagt tttgcttcgg aacagctgga tttgactatt gatccggaga
    23521 gtaaagggat tcggattatc acattgcgtt cgccgctgta tgtacgtggg acgtttaaaa
    23581 atccgcaggc tggggtgaaa gccgggccgt tgattgctcg cggagccgtt gcggcggcac
    23641 tggcaacgct ggtaacaccg gcggcggcgt tactggcgct gatctcacct tccgaagggg
    23701 aggctaatca gtgcaggaca attttgtcgc agatgaagaa gtgatgataa ttgccggatg
    23761 tctgacatcc ggcaattaaa cagatttaga ttgttggcaa agtgtgcttt gttcatgccg
    23821 gatgcggcgt gaacgcctcc ggcttacaaa tccttgcgaa ttcaatatat tgtatggaaa
    23881 ttctggcctg ataagcgtag cgcatcaggc ttttcactct tattacaacg actgatgtcg
    23941 cgtctcatgg gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatatag
    24001 ccccaccgca cctaacccgt agttagtctg caaccaggct gcgatatacg gcgcaacgga
    24061 agccccgaga atcgacgata cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt
    24121 cgggaacagc tctggtaaca gcgcgcccat cggaccgaag gtcagcccca tcagacttaa
    24181 ccccagcagc agaaaggcaa aaaccagaat cgggttgcca gaaccgagca aaggggtaaa
    24241 ggcgaatagc gcgaacagga tgatcagcgt ggtgatgatc accatgcttt tacggcgacc
    24301 aaaggcatca gccagtaatc cggcgactgg caccatcacg ccaaaaccaa taactgccat
    24361 catcagcatc cacaacactt cgttacgcgg caggccaagc ccaactggcg cggcggcggt
    24421 actaaaggtc atagagtaga ccgtcatgat gtaaaacagc gtataggttg ccagcataat
    24481 gaacgtaccc agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt
    24541 cacctgtttt ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg
    24601 aacatacagg ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca
    24661 gctcataaac tgctcgtcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa
    24721 gaagaagcca atcggtgcgc ccagctgcgg gaaggagccg tacaatgcac gtttgcgcgg
    24781 tggggcgttt tcagtcgcca gcagcgccgc gccgccccat tcaccaccta agcccagacc
    24841 ctgaccaaat cgagccagcg ccagcagcag cggggcgaaa ataccaatcg tggcatagcc
    24901 tggcagcaga ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt
    24961 cgctttacgc ccaacgcgat cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc
    25021 gacgaaggcg atggcgaagg tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg
    25081 cgggaagaag atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa
    25141 gaactcaatg gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg
    25201 cgtgtattct tgctcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt
    25261 tttggctaac gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat
    25321 cgcggaaatg gtcaaaaatc aggcaaaaag ggaataatgt ttaggccagt gatttttctt
    25381 ctatgaaatg cttcacataa ttttataaat ggtggattaa actggtttta ggttaaataa
    25441 aagttaccaa ctggatttat atgctggggt tatgagttgg aatgaaattg tttgctcctg
    25501 aatagatggc taaagaggct ctgggaaaga gtttcaagag gttatgtgcg cattatagcc
    25561 ggatgcgatg ctggcgcatc ttatccggcc tacggggagc caggccggat aaattcgtac
    25621 gccgcatccg gcctatttta aggctgtgtt ttccccggca ttcgcgggtc gtctttatat
    25681 tcggcggtgg caatccacgc cgcgcaaaac agcgtcaaac gggcgaagaa gtagaaaaat
    25741 gccatcagcc ccagcacgga accaaatgct gcgccagagg gtgatttcat caacgatggc
    25801 agggtgtagg tcatcacgat tttaatcact tcaaaaccaa tagcagccag aaatgttccg
    25861 cggatcaggg cttttttgcg cggacggtgg cgcggcaacc gccagaagat ccagaagaaa
    25921 agcagatagt tggcgaagat ggaaatcgcc acgccaatca accgccatgt cggtttcagc
    25981 cactcaatgc tgttcaggtg cagggcgcta ataatcattt gctgcgcaga accggcaacc
    26041 gaggtgatag aaagcgtcac aatcagcgca atcaacaaac caatcagcga aataaaatca
    26101 cgcagatatt ttacccagaa cttctcctga tcttgcggcg agcgttccca gacatcgcgt
    26161 gactgggcgc gaatcgcttc acgcaggtta cccatccagt tgatgccgga ataaagtgcc
    26221 actgccagac cgacaagccc tacagtcgta cgctgctgaa cggcggtgtt gatggtgttt
    26281 ttcaatgtgg cggctagcgt cggatcgctg atgttttgca gaattttgtc gaagatatcc
    26341 tgtagcaaca tcggatggga ggcgagcaca aagccccctg cggcaaacga caccatcaaa
    26401 atggggatca tcgacaaaaa tgagaaatag gtgatagccg caccaaactg gttacccagc
    26461 cgatcgttaa agcgttctgt cgcgcgaatc aggtgcgcaa taaccggttg gcgttggact
    26521 ttctcggcag tggtggtgac ggtttccagc gcctggctaa ctttgccgcc tttctcgcta
    26581 ttatccaact gcttaatcgg ctcgtgctcc agatcctggg tgggacgttt gatctcgttt
    26641 tcctgcgtca tccgctcttt tgtccttctg tcgtggtcat tcttaaaagt atagtcggtc
    26701 agtccaggta ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg
    26761 aaagctcccg acgctgcgga taaaccagtg aaagggacag cggctcggca cggtagccag
    26821 gcaacacttc aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct
    26881 gaataatccc cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca
    26941 acatgctgcc agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg
    27001 gatgtacacc caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc
    27061 tttgcggata accaaagcgc gtcagatagt gcggactggc acagttgacc atggtcagtt
    27121 taccgagggg acgggcgata acgccatctt ccggtaatgc gccagtgcgt atcacgcaat
    27181 caaaaccatc atgaagaata tctaccggac ggtcatggct actcagttcc agctcaattc
    27241 ccggatgcag atagagaaat tccgacaagc gcggcagtaa caggcttttc gcgattcctg
    27301 acgggatgtc gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt
    27361 ccagttcgct gagattactc aacacatctt ttgctcgttg ataataggtc atgccttctg
    27421 gcgtgagttt gacccgtcgc gtggtgcgct ggagaagctg ggtgccaagt tgatgttcca
    27481 gtgcctgtat ctggcgcgaa acacttccct ttggcaaagc aaagaaatcc gccgcgcggg
    27541 aaaaactttc cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt
    27601 ctttgaccga ttgttgttca caacgaaaca gtagcgtgca tatcgcattg tttattgttt
    27661 tttctaacat ctaatgccgg gtttcacaat gttgtcatga tgaatgtggc gatttctcag
    27721 caggaaaagg gaggaatagc tttatgagaa aggtaaattc ccttaaacag agcgtatttt
    27781 tcagcgattc cctttaaagt gaatagaaga tgtattggct atttgtgctt atttttatct
    27841 cattgccgtt tgttattttt gtttatggtc gcggaatttc atttttaatt gcatgcgtac
    27901 taactaccga tataaggaaa tcctcaaaaa tgttttaaat agtgccctcg acatgtaatt
    27961 tcaggcgttt agctgtaact atttatatcg gtaactattt tctacgtgtc atctctgtag
    28021 aatggccacc gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac
    28081 ttaagtcttt ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag
    28141 gcagtcaata tttattcatg gaatgaaaat cagtttacag catcgtattc caggagtgag
    28201 tattcagggg gccagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc
    28261 cttagtgatg ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac
    28321 tgtggtgcaa tttcctgagg ttaaggtgtt aattacggcg acggattgca acaaacggtg
    28381 gttacaggaa gttattcatt ttaatgtgct ggccattgtg cctcgtgatt caaccgtcga
    28441 gacatttgcg ttggcggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg
    28501 gcgaacgacg ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga
    28561 gattttaacc atgttagcgg cgggcgaatc aaataaagag atcggcaggg cgctgaatat
    28621 cagtaccgga acggtaaaag cgcatctgga atcactgtat cgccgtctgg aagtcaaaaa
    28681 tcgcacccag gcggcaatga tgttaaatat ctcctcctga agaggataat tttacagcca
    28741 gcaggaagtt agcggctggc tgtaaaaata ttatggttcg ccgtacaaac caattaaacg
    28801 gcgtaccaca ccgttagtcc agccaaaccc atcctgcaac ggatactcgc caccgccgcc
    28861 ttcgcgagga acaccatcgg caatatggta tttctcgatc agtttgtgct gttccagata
    28921 gaactgattc accgtcttca gccagttgcg cgcgatttca tcacccagaa ggtcatcgcc
    28981 gtacatctta aatccctgaa ttgccatcca ctgtaacggt gcccagccgt tgggtttatc
    29041 ccactgttca ccggtttcgt actcgcttgc cagaatcccg ccaggtgtca gtaaccggct
    29101 gcgcacggcg tttgccagac gatcggcctg ttcatggttc gccataccga catagagcgg
    29161 cacaatggcg gcagcggaaa acagcgccag ttgctcgcgt cgccagtcgt aatcgcggta
    29221 gataccgttt tcatcatccc agaggtaacg gtttaccgca tcgcgacggg cactggcctt
    29281 ctggcggaac agtgcttctg tctctttctc gcctttcagt gccgagatgt tggcgatggc
    29341 gctctccagt ttaaacagga aggcattcag atcgatgggg atgaactggg tggtacgaat
    29401 gctcgccaga cgaccagtat cacgcagcca acgggaagag taatcccaac cggaggccgc
    29461 ccccgcacgt aaatcgcggt acacctcgtt aggcgggcga ccagaatgtt tcgcggtttc
    29521 aacgtcctca agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccagt aacggttgag
    29581 cagcgagccg tccggcatcc gcacaacatg gcgataggcc tgattaggga tcaacgattc
    29641 tgcaccgtcc atccagaagg catattccat tttcaggtgg tcgagatagc ggcgcgcgcc
    29701 gcgtacacca tcttcttcaa acaactccac catcagcgca aaaaccggtg gttgcgagcg
    29761 gctcaaataa taggtgcggt tgccgttggg gatgtggccg tagttttcga tcatccaggc
    29821 gaagttatcg gccatgcatt tcagcaaatc ttcccgacca ctttccgcca gccccagcat
    29881 ggtgaaatag gaatcccaat agtaggtttc gctaaaacgg ccgcccggga caatatatga
    29941 ctgcggcagc gccagcagag aagaccacgg aatgtgatcc tgtggttcac gggttagcac
    30001 cggccacagc tggtcgatat gctctttcag ggaattttgc gggtccgata catactcgct
    30061 ggagtagacc tccggcagcc agaagtggtt ttcaacaaac ttgcgcaagt caaaatcacg
    30121 gtgacggcgc actttacggt agcggattaa aatatccagc gggtccattt tgggtgcgca
    30181 gtcgggaaag gttttactgt cggggaaaat tttcgccgac tgaacatgct cgaacagttc
    30241 gagatagcga tcggcaggcg tcagcgcatc agaggcgggc agcccttcaa tcatctcggg
    30301 ttccggttct gcctcgatca tctcatccag ttttaattca tacgggtcca gctcatagca
    30361 gagatcgacc tcgatcatca gttcgtctgg attagggttt tgaattttct gattgagcat
    30421 aaggatccga cctccgaaat tcgtcgctaa ttatcgggct tttctttaag ggtagtcatt
    30481 cgctccgcct ggtgggcgac aaagcgtgcg gtggatcacg tttattaacc tgccaaaaaa
    30541 tataacgcac taatatcaat ggaatttata aaaatacgga tttcgtgttg tttttcattt
    30601 tcagaacatt caatcctgct ctcgccgcaa tcatgatatg ccattcctga tctgggtaat
    30661 aggcactggc tatcttattg atagtttata ttcatgtaat tgattgattc ctatcaaaaa
    30721 gcatatctcc ctgtttttta tgatggcttt gtctcgcaga gacgtttgca gcattctgga
    30781 aaattttctg ggtcacaata actcactacc gtgagggaag aataatgaaa atggtctcac
    30841 gtattaccgc gatcggcctg gctggcgtcg cgatttgcta tttagggtta tctggttatg
    30901 tgtggtacca cgataataaa cgcagtaaac aggccgatgt tcaggcatct gctgtcagtg
    30961 aaaataataa ggttttaggc tttctccgcg aaaaaggatg cgattattgc catacgcctt
    31021 cggcagaatt acccgcctat tattatattc ccggcgcgaa acagttgatg gattacgaca
    31081 taaagcttgg atataaatct tttaacctcg aggccgtgcg tgcggctctg ctggctaata
    31141 aacccgtttc gcaaagcgat ctgaataaga ttgaatgggt gatgcagtat gaaactatgc
    31201 cgccaacgcg ttataccgcg ctacactggg cgggtaaggt gagtgatgaa gagcgggcgg
    31261 aaatactggc ctggattgca aaacagcgcg cggaatatta cgccagcaat gatactgctc
    31321 cggagcatcg caatgaaccg gtgcagccca tcccgcaaaa actgcctacc gatgcgcaaa
    31381 aagtggcgtt gggttttgcg ctgtatcacg atccccgttt atcggcggat agcaccattt
    31441 catgcgctca ttgccatgcg ttgaatgcgg ggggcgtcga tggcagaaaa acatcgattg
    31501 gtgttggtgg cgcagttggg ccgattaacg cgccgacggt atttaactca gtatttaacg
    31561 ttgagcagtt ctgggatggt cgtgcggcaa cattgcagga tcaggctggt ggaccgccgt
    31621 tgaacccgat tgaaatggcg tcgaaatcct gggacgaaat tattgctaag ctggaaaaag
    31681 atccgcagct taaagcgcag ttcctcgaag tctatccgca aggtttcagt ggcgaaaata
    31741 ttactgatgc cattgctgaa tttgagaaaa cattaattac gccggattcc ccatttgata
    31801 aatggttgcg tggagatgag aatgctctga cggcgcaaca gaaaaaaggc tatcaattat
    31861 ttaaagataa taaatgtgca acttgtcatg gtggtattat tctcggcgga cgttcctttg
    31921 aaccgttggg gctgaaaaaa gactttaact ttggtgaaat tacggcggcg gatattggtc
    31981 gtatgaatgt tactaaagaa gagcgtgata aattgcgtca gaaagtaccc ggtttacgta
    32041 acgttgcttt aacggcaccg tacttccatc gcggtgacgt gccgacgctg gacggggcag
    32101 tgaaactgat gctgcgctat caggtaggca aagagctgcc gcaggaggat gtggatgata
    32161 tcgtagcttt cctgcacagt ctgaacgggg tttacatgcc gtatatgcag gataaacaat
    32221 aattaatttg atcgcccgaa caggagtgtt tgggcgattt ttattacgat aataaagtct
    32281 gtttttaata ttatcatatt aaatgtttat attataaaag gtcgtttttc tgcttaggat
    32341 tttgttattt aaattaagcc tgtaatgcct tgcttccatt gcggataaat cctacttttt
    32401 tattgccttc aaataaattt aaggagttcg aaatggacca gaagctgtta acggatttcc
    32461 gctcagaact actcgattca cgttttggtg caaaggccat ttctactatc gcggagtcaa
    32521 aacgatttcc gctgcacgaa atgcgcgatg atgttgcatt tcagattatc aatgatgaat
    32581 tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc tgggacgacg
    32641 aaaacgtcca taaattgatg gatttgtcga tcaataaaaa ctggatcgac aaagaagaat
    32701 atccgcaatc tgcagcaatc gacctgcgtt gcgtaaacat ggttgccgat ctgtggcatg
    32761 cgcctgcacc gaaaaatggt caggccgttg gcaccaacac cattgg
//
LOCUS       DAOFSV010000040        31768 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.040, whole genome shotgun sequence.
ACCESSION   DAOFSV010000040 DAOFSV010000000
VERSION     DAOFSV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.040"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(144..1319)
                     /gene="ubiF"
                     /locus_tag="QBH27_001998"
     CDS             complement(144..1319)
                     /gene="ubiF"
                     /locus_tag="QBH27_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDQ7014310.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLTALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            1465..2889
                     /gene="miaB"
                     /locus_tag="QBH27_001999"
     CDS             1465..2889
                     /gene="miaB"
                     /locus_tag="QBH27_001999"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDQ7014311.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3042..4082
                     /locus_tag="QBH27_002000"
     CDS             3042..4082
                     /locus_tag="QBH27_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDQ7014312.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4079..4546
                     /gene="ybeY"
                     /locus_tag="QBH27_002001"
     CDS             4079..4546
                     /gene="ybeY"
                     /locus_tag="QBH27_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDQ7014313.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4636..5514
                     /gene="corC"
                     /locus_tag="QBH27_002002"
     CDS             4636..5514
                     /gene="corC"
                     /locus_tag="QBH27_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDQ7014314.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5539..7077
                     /gene="lnt"
                     /locus_tag="QBH27_002003"
     CDS             5539..7077
                     /gene="lnt"
                     /locus_tag="QBH27_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDQ7014315.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSTAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYVQWFTPQP
                     EKTIQVSMVQGNIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLITGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYSRTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7204..9081)
                     /locus_tag="QBH27_002004"
     CDS             complement(7204..9081)
                     /locus_tag="QBH27_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDQ7014316.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDANTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            9535..10443
                     /gene="gltI"
                     /locus_tag="QBH27_002005"
     CDS             9535..10443
                     /gene="gltI"
                     /locus_tag="QBH27_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDQ7014317.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGNTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10613..11353
                     /gene="gltJ"
                     /locus_tag="QBH27_002006"
     CDS             10613..11353
                     /gene="gltJ"
                     /locus_tag="QBH27_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDQ7014318.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11353..12027
                     /gene="gltK"
                     /locus_tag="QBH27_002007"
     CDS             11353..12027
                     /gene="gltK"
                     /locus_tag="QBH27_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDQ7014319.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12027..12752
                     /gene="gltL"
                     /locus_tag="QBH27_002008"
     CDS             12027..12752
                     /gene="gltL"
                     /locus_tag="QBH27_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDQ7014320.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            12870..13805
                     /gene="rihA"
                     /locus_tag="QBH27_002009"
     CDS             12870..13805
                     /gene="rihA"
                     /locus_tag="QBH27_002009"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDQ7014321.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            13889..15559
                     /gene="hscC"
                     /locus_tag="QBH27_002010"
     CDS             13889..15559
                     /gene="hscC"
                     /locus_tag="QBH27_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDQ7014322.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVNASAGDNFLGGEDFTHMLVDEVLKRA
                     EVAKTTLNDSELAALYACVEAAKCSNQSPMHIRWQYQDEMRKCEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRNEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGTYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15619..17070)
                     /gene="djlC"
                     /locus_tag="QBH27_002011"
     CDS             complement(15619..17070)
                     /gene="djlC"
                     /locus_tag="QBH27_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDQ7014323.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAATEHEILRVFRALLDSENDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDCEGIDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFSLERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLDARLERGEAHPLETIITRMLLTKVKLEPEDEDVDEPTPENHEEIND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(17067..17774)
                     /locus_tag="QBH27_002012"
     CDS             complement(17067..17774)
                     /locus_tag="QBH27_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDQ7014324.1"
                     /translation="MNKEEQYLLFALSTPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            17876..18430
                     /locus_tag="QBH27_002013"
     CDS             17876..18430
                     /locus_tag="QBH27_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ7014325.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLSAH"
     gene            18570..18905
                     /locus_tag="QBH27_002014"
                     /pseudo
     CDS             18570..18905
                     /locus_tag="QBH27_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(18975..19457)
                     /locus_tag="QBH27_002015"
     CDS             complement(18975..19457)
                     /locus_tag="QBH27_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDQ7014326.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            19692..22274
                     /gene="leuS"
                     /locus_tag="QBH27_002016"
     CDS             19692..22274
                     /gene="leuS"
                     /locus_tag="QBH27_002016"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDQ7014327.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            22289..22870
                     /gene="lptE"
                     /locus_tag="QBH27_002017"
     CDS             22289..22870
                     /gene="lptE"
                     /locus_tag="QBH27_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDQ7014328.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            22870..23901
                     /gene="holA"
                     /locus_tag="QBH27_002018"
     CDS             22870..23901
                     /gene="holA"
                     /locus_tag="QBH27_002018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDQ7014329.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            23903..24544
                     /gene="nadD"
                     /locus_tag="QBH27_002019"
     CDS             23903..24544
                     /gene="nadD"
                     /locus_tag="QBH27_002019"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDQ7014330.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            24568..25179
                     /locus_tag="QBH27_002020"
     CDS             24568..25179
                     /locus_tag="QBH27_002020"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDQ7014331.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQQAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            25439..25756
                     /gene="rsfS"
                     /locus_tag="QBH27_002021"
                     /gene_synonym="rsfA"
     CDS             25439..25756
                     /gene="rsfS"
                     /locus_tag="QBH27_002021"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDQ7014332.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            25760..26227
                     /gene="rlmH"
                     /locus_tag="QBH27_002022"
     CDS             25760..26227
                     /gene="rlmH"
                     /locus_tag="QBH27_002022"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDQ7014333.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            26258..28159
                     /gene="mrdA"
                     /locus_tag="QBH27_002023"
     CDS             26258..28159
                     /gene="mrdA"
                     /locus_tag="QBH27_002023"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDQ7014334.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFTVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28162..29274
                     /gene="mrdB"
                     /locus_tag="QBH27_002024"
                     /gene_synonym="rodA"
     CDS             28162..29274
                     /gene="mrdB"
                     /locus_tag="QBH27_002024"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDQ7014335.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            29285..30373
                     /gene="rlpA"
                     /locus_tag="QBH27_002025"
     CDS             29285..30373
                     /gene="rlpA"
                     /locus_tag="QBH27_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDQ7014336.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            30513..31724
                     /gene="dacA"
                     /locus_tag="QBH27_002026"
     CDS             30513..31724
                     /gene="dacA"
                     /locus_tag="QBH27_002026"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDQ7014337.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
BASE COUNT         7849 a         8219 c         8095 g         7605 t
ORIGIN      
        1 ctcgcttcgg cgagcttttt gcttttctgc gtatattcaa tgccgaatac gatgttgact
       61 cgtcttatcc ttacaaagtc ggatgcgacg ctgccgcgtc ttatccgacc tacggttggc
      121 atgcatccag caatgttgtg aggctacaac cctaacgcat atttcagcgc cttacgtttc
      181 aacacgccag cacgctccgc cgccatcaaa ccaagattac gcacaaaacg cagcggtggc
      241 agattattgc tgaatccggc ataaaacaga tccataccac tttgcatcat gaagttatcc
      301 gccatacgcc gcatctggta acgcttgagc acaggataac tggcccacgc ttcgccgtag
      361 ctgcgggcgt tcaccagaac gtcaatcagg gcatcgacat cacgataacc aagattcact
      421 ccctgccccg ccagcggatg gatggtatgc gcggcatcgc ccaccagagc aagccctggt
      481 tgcacatact gcaacgcatg gcgacgtata agcggaaacg caccagcggc aagcggtgtc
      541 acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg cctgtagctg cgccatattc
      601 atattctgca actggcgaat gcgcgccgga gagtcatacc acaccagcga tgcccagtta
      661 tcaaacaacg gcagaaacgc acgcggtccg tccggggtaa attgctgcca ggtgctgtcg
      721 ccgggatcgt tctcacactg tacgctaatc aacatacacg actgcgcata ctgccaggca
      781 tgaacgccaa ttcccgccat ctgtcgcacc tgcgaatttg cgccgtcggc accaatcacc
      841 agcttcgcga ggatcgtttc accgcccttc agttccagct cctgaagatc attatggcga
      901 tgcagtgcag tcagcgaggt tggcacacgt aacgttactt tcggatgcgc ttccagcgcc
      961 tgccacaaag cctgttgcag gacagtgttt tccaccatgt agccaagcaa cggtagctta
     1021 agttcagcgg cgtcaaacac gacatgcgcc gtttcccact cccacgtttc cagtctgcgg
     1081 taaggatggc aacgcatagc ctgtactgca tcccagaccc ctaacccttt aagcaatgat
     1141 accgaagccg cgctgatcgc cgagatccgc acatccggtt ggctgtcagc gacaaacggc
     1201 gcaggttctg cgtgctcgat caccgttacc gtaaacccgt gctgtgccag ccccagcgcc
     1261 agtgcgccgc cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg
     1321 gtcaatcatc ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga
     1381 taacgctcat actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg
     1441 ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt
     1501 cagatgaacg agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat
     1561 caactgaccg acgtggcgga agaagcggat gtgctgctgc tgaacacctg ctcaatccgc
     1621 gagaaggctc aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag
     1681 aatccagacc tgattatcgg cgtcggcggc tgcgtggcat cgcaagaagg cgagcacatt
     1741 cgccagcgcg cccactatgt cgatattatt tttgggccgc aaacgctgca tcgtctaccg
     1801 gagatgatca actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa
     1861 atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc
     1921 atcatggaag gctgcaataa atattgcacc tactgcgtgg tgccttacac ccgtggtgaa
     1981 gaggtaagcc gtccgtccga cgatattctg tttgagatcg cccagcttgc ggctcagggc
     2041 gtgcgtgaag tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga gaactacgac
     2101 ggcaccaccg gaacgtttgc cgatctgctg cgtctggttg cggcgattga cgggatcgat
     2161 cgtattcgct ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtat
     2221 cgcgacacgc cggagctggt gagcttcctg cacttgccgg tacagagcgg ttccgatcgc
     2281 attctgaacc tgatgggccg tactcacacg gcgctggagt acaaagcgat catccgtaaa
     2341 ctgcgtgcgg cgcgtccgga tattcagatc agttctgact tcatcgttgg cttccctggc
     2401 gaaaccaccg acgacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg
     2461 agctacagct ttatcttctc cgctcgtcca ggaacgcctg ccgccgatat ggttgatgat
     2521 gttccggaag aagagaagaa gcagcgtctg tatattctgc aagagcgcat taatcagcaa
     2581 gcgatggcat ggagccgccg tatgctcggc accacccagc gtattctggt agaaggtaca
     2641 tcgcgtaaga gcatcatgga gctttccggt cgtactgaaa ataaccgcgt ggttaacttc
     2701 gaaggcacgc cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctatcca
     2761 aactccctgc gcggaaaagt ggtgcgtact gaagatgaaa tgggtctgcg cgtcgcagaa
     2821 acaccggaat cggtgattgc ccgtacccgc aaagaaaacg accttggcgt gggttattat
     2881 cagccgtaat tctcaggccc gccgttccgg tgggccttgt tttttctctc tttatcccca
     2941 tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt
     3001 gaatgttcag cttccggcgg catacagata agaggaacgg tttgaacata gacactcgcg
     3061 aaatcaccct ggagccagca gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg
     3121 acaacatcaa acaactcgaa cgccgtctcg gcatcgaaat caatcgccgt gataaccact
     3181 ttaaactgac cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt
     3241 atgtcgatac tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc
     3301 ttgcgattaa agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg gagtacggca
     3361 aagcagtcaa tatcaaaacc aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc
     3421 agtacatcgc caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg
     3481 gtaaaaccta cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc
     3541 gtattctgct gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg
     3601 atttaagcca gaaagtagac ccgtatctgc gcccgctgta cgacgcgctg tttgaaatgc
     3661 tgggctttga gaaagtcgag aaactgattg agcgcaacgt tattgaagtc gcaccgctgg
     3721 cctatatgcg tggtcgtacg ctgaacgacg cgtttatcat tcttgatgag agccagaaca
     3781 ccaccatcga acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta
     3841 tcaccggcga cgtcacacag atcgacctgc cgcgtaatac caagtctggt ttgcgtcatg
     3901 ccatcgaagt gctggctgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg
     3961 tggttcgtca cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg gaagaagccg
     4021 aacaaaaacg taaagcggcg ctggcggcag aacgcaagcg cgaagaacag gaacaaaaat
     4081 gagtcaggtg atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga
     4141 gagccagttt cagacatggc tgaatgcggt gatcccgcag tttcaggaag aatcggaagt
     4201 gactattcgc gtggtcgata ccgccgaaag ccacagtctg aatctgacct atcgcggtaa
     4261 ggataagccg accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggaaatgtc
     4321 gctactgggc gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg
     4381 caaaccactg gaggcgcact gggcgcatat ggtggtgcac ggcagtctgc atttgttagg
     4441 ttacgatcac atcgaagatg acgaagcaga agaaatggaa gccatcgaaa cagagattat
     4501 gcttgctctg ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt
     4561 gactggcaga cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag
     4621 aacccatacg acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag
     4681 aagggatttt tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac
     4741 gaactgctgg cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc
     4801 gatatgctcg aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc
     4861 cgctcccaga tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc
     4921 atcgagtccg cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg
     4981 attctgatgg cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcagcatg
     5041 gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg
     5101 aaagagtttc gctctcagcg ttaccacatg gcgatcgtca ttgacgaatt cggtggggtt
     5161 tccggtctcg tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag
     5221 tatgacgaag aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc
     5281 gcactggctt ctattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagag
     5341 gttgacacta tcggtggtct ggtgatgcag gcatttgggc atcttccggc gcgtggcgaa
     5401 accatcgaca tcgacggtta ccagttcaaa gtggcgatgg ccgacagtcg gcgtattatt
     5461 caggttcatg tcaaaatccc ggatgactca ccccagccga agctggatga ataaaaccga
     5521 aactggatag ataactacat ggcttttgcc tcattaattg aacgccagcg cattcgcctg
     5581 ctgctggcgt tattattcgg tgcctgcgga acgctggcct tctctcctta cgacgtctgg
     5641 cctgcggcga ttatttcgct gatgggactt caggcgctga cctttaaccg ccgtccactc
     5701 cagtctaccg ctattggctt ttgctgggga tttggcctct ttggcagcgg tattaactgg
     5761 gtctatgtca gcatcgcgac ctttggcgga atgcctggcc cggttaacat cttcctggtg
     5821 gtactgctgg cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt
     5881 ctgtggccga aaaccacctg gctgcgcgtg gcgattgccg cccctgctct ctggcaagtg
     5941 accgagtttc tgcgcggttg ggtactaaca ggcttcccgt ggttacagtt cggctatagc
     6001 cagattgatg gcccgttaaa agggctggca ccgataatgg gcgtggaagc cattaacttc
     6061 ctgctgatga tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg
     6121 ctggtggtgg ccgtcgtgct gtttgccctt cccttcccgc tgcgttacgt ccagtggttt
     6181 accccgcaac cggagaaaac cattcaggtt tcgatggttc agggcaatat tccgcaatcg
     6241 ctgaaatggg acgaaggcca gcttcttaat acgctgaaga tttactacaa cgcaacggca
     6301 ccgctgatgg gcaaatcatc gctgattatc tggccggagt cggcgataac cgatctggaa
     6361 attaatcagc aaccgttcct caaagctctg gacggtgagt tgcgcgataa aggtagctcg
     6421 ctgatcaccg ggattgtcga cgcgcgtctc aataaacaga accgctacga tacctacaac
     6481 accatcatca cactgggtaa aggcgcaccg tacagctatg aatcagccga tcgctataac
     6541 aaaaaccatc tggtgccgtt tggcgagttt gtcccactgg agtcgattct gcgtccatta
     6601 gcaccgttct ttgatttgcc gatgtcgtca ttcagccgcg gaccatatat tcagccaccg
     6661 ctgtcgttga atggcattca acttactgcg gccatttgct acgaaatcat tctcggcgag
     6721 caagtgcgcg ataacttccg cccggatacc gactatctgc tgactatctc caacgatgcg
     6781 tggtttggta agtctattgg tccatggcag cacttccaga tggcgcgaat gcgtgcgctg
     6841 gagctggcgc gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg
     6901 cagggtgaga ttcaggcgat gatcccgcag ttcacccgcg aggtgttaac cactaacgta
     6961 acgccgacca ccggactcac cccgtactca cgtaccggca actggccact gtgggtgctg
     7021 acggcactgt ttggctttgc cgctgtactg atgagtctgc gtgctcgcaa acgttaatgt
     7081 gcctgatgcg cgacgcttat caggcctacg cgatttttgc aatatgttga attttcgcga
     7141 ttttgtagga taaagcgtac tttttcaaca cgctctgggg catgattatc atgccctttt
     7201 ttcttacggt tcagggatac aagccacatc gccatattgg ggatgccgaa taattgcctg
     7261 cggatcaagt gaactttctc tgtccagttg tattagcccg ttcttcgcca taacaaatat
     7321 ctttctacca tccgctgaaa aacgaagatg attcacgtag ctggcaggat aaacgccaat
     7381 aatctgcccg gcattaatat tgagcaggat gacataacct tgcaggccat atgacaccaa
     7441 cgctaaatat tgaccgtccg ggctaatacc ctcaacggaa taaacgctcc tgctacacca
     7501 ttgcgtcata atgctggctt gttgatcctc agctcttata cgtgaaatcc cctggctatc
     7561 ggcaaaaata atctcaccat tttttggtcg gaatcccatc gcagaaacga caccacgcgc
     7621 atcacctgcg ggaaagtctt ctttctgatg cgtgatggta ttgatccgca cgatgttacc
     7681 tctgcctgct cccggaacca gtaatgtttt ttcatccggg gaaagtgcca taacccaggc
     7741 gcctggttta ccgctcatcc ccgtcccgtc gtatttctcc agcgggatat ccgccacatg
     7801 ctgataagcc accagatcaa tcgctgacac actattactg gtgcgattca tcacccaggc
     7861 ttttgtgcca tcacgcgaga ggataaggct gtcaggatgt gcgccggtgg taatcgactg
     7921 aataatctcc cccgttgcca cgttaatgac cgataatgca ttcgcttcaa atgaagtcgc
     7981 atacagcaac gtttcatccg ggcttagcgc cagcgacctg acgccctgat ctttacagcc
     8041 accacaatgt ttgactgact tttctttcgc tatgggggct gtaaaggtat gcaggatttt
     8101 cttttgtccg aggtcgaaaa caacaatgct gttcgtgttt tcaattgcgg catacaacgt
     8161 attagcatcc cgactgatcg ccatatcatg gatatcggca atctcagcga gtaaatcacc
     8221 actggcctgc tcacgactta ccggagcgga taatgtttcc ctgagggcgt tgcgctcctc
     8281 cactaactgc tgcttttttt gtcgtcgctc ttgttcaatc tcctgcggat aaaacgcttc
     8341 acgcaggctt tgcctgacct gtaaaactga ttcatcaatc tgctgctgca caagccagat
     8401 agtcccggtc agaagaagag tgaccgcaac aataatcccg ccttccgtaa cgagccgatt
     8461 ttgcccaact aaacgcgcac tcagccaacc caacgcgccc cccgcaatca ggccgccaat
     8521 gtggcaagcg ttatctattc ctgtttgccg ggtaccatac aacagtgtca gcgccaccat
     8581 acccaacaga ggaaatactc gccgctggtt tttatggtgt gtgcccgcac ctgaagcaag
     8641 ctgtgttgct atcgcagcgc ccgctattcc cataattgcc ccggatgccc caacgctgat
     8701 gactatctgc tcactttcgc gtaacgcata acaggcgctg atcagcccac caaagatgcc
     8761 ggagaataac cagataatca ggagtcgcca tttccccagt atccgttccg ccactgtccc
     8821 caccaccagg agtgcaaaca tattcatcag caaatgggaa aaactactgt gcagaaaaac
     8881 actgctcaat aagcgccagg attcatccga caacgtgaga gaagcaacgt tagcgccgag
     8941 atggatgagt gcggattgat cgacttcttc gtaactcatc cattgtgcga ggtaacaaaa
     9001 tatcccaaca cagagcgcaa aaaggatcag ggttattgcg ggtaattgaa catttaacgg
     9061 cttaaccgaa gatgcagaca taaggtgatt cttccttgaa aagactagta aaaagcattt
     9121 tcccgtgtgt agttatcatc ccggcaaacg tcccacaccg aaaagttaac actgtccgaa
     9181 atatgacttt ttcaaactga aattacaatc gtaattaatt cacccctaag tattttttgg
     9241 catgtaagtt gctttattta ctgtgtaagc aatgaaatca gccgcaacgc aaccgggttg
     9301 caccaccaca aatcagcggt agcaccaaaa aggtgcggtt taagaatttt gcttcggaat
     9361 ggtgcggcgc acctcgcaaa aacaaacaat aaaacagcaa tatctttaca tttatccaaa
     9421 ctaaatgtta cctaacaagc acaacactgc acaataaagt cgcagatgat aacaacacaa
     9481 acactcacaa cgggtatcca tgcgttttta acgcagaaga taaaggagtt ggatatgcaa
     9541 ttacgtaaac ctgccacagc aatcctggcc ctggcgcttt ccgcaggact ggcacaggca
     9601 gatgacgccg ccccggcagc tggcaatacg ctggacaaaa tcgccaaaaa cggcgtgatt
     9661 gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg
     9721 gtgggctact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
     9781 aaaccagact tacaggtgaa attgattccg atcacctcac aaaaccgtat tccactgctg
     9841 caaaacggca ctttcgattt tgaatgcggt tctaccacca acaatgccga acgccaaaaa
     9901 caggcggctt tctctaacac tattttcgtg gtcggtacgc gcctgctgac caaaaagggc
     9961 ggtgatatca aagattttgc cgacctgaaa ggcaaagccg tagtcgtcac ttccggcacc
    10021 acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc
    10081 agcgccaaag atcacggtga ctctttccgt accctggaaa gcggtcgtgc cgttgccttt
    10141 atgatggatg atgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg
    10201 gaaatcgtcg gcaagccgca gtctcaggaa gcctatggtt gcatgttgcg taaagacgat
    10261 ccgcagttca aaaagctgat ggatgatacc atcgctcagg tgcagacctc cggtgaagcg
    10321 gaaaaatggt ttgataagtg gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat
    10381 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac
    10441 taattacaag aaccaggggc ggaaattcca gccctctcga ttgttacgta gcacggacag
    10501 actatacgcc tggtggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa
    10561 tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat
    10621 agactggaac tggggtattt ttttacaaca agccccgttc ggcaacacca cctatcttgg
    10681 ttggatctgg agtggctttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc
    10741 tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg
    10801 tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac
    10861 ctggtaccta gtgatcccgg agctgctgcc agagaaaatc ggcatgtggt ttaaggcaga
    10921 gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc
    10981 cgcccgtgtt tgcgaacagg tacgcgcagc tattcagtcg ctgccgcgtg ggcaaaaaaa
    11041 tgccgcgctg gcgatgggtc tgacgctacc gcaggcttac cgctacgtac tgctgcctaa
    11101 tgcttatcgc gttatcgtcc cgccgatgac ctcagagatg atgaacctgg tgaaaaactc
    11161 cgccatcgcc tcgactatcg gtctggtgga tatggcggcg caggcgggta aattgctcga
    11221 ttactcggcc cacgcctggg agtcgtttac cgccatcacg ctggcgtatg tattgattaa
    11281 cgcttttatc atgctggtga tgacgctggt tgaacgtaaa gttcgcctgc ccggcaacat
    11341 ggggggcaaa taatgtacga gtttgactgg agttccattg ttccttccct gccatatctg
    11401 ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg cagtggtgat tggtattttg
    11461 tggggcacca tgctggcggt gatgcgcttg tccagctttg cgcccgtagc gtggtttgcc
    11521 aaagcctacg ttaacgtatt ccgctcgatt cctttagtta tggttttgct gtggttttac
    11581 ctgatcgtac cgggttttct gcaaaacgtg ctgggattat cacccaaaaa tgatatccgt
    11641 ttaatttcgg cgatggtggc gttttcgatg tttgaagcgg cctactattc agagattatc
    11701 cgtgctggta ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc gttgggaatg
    11761 actcactggc agtcgatgaa actgattatt ctgccgcagg cgttccgcgc gatggtgccg
    11821 ctgttgctca ctcagggcat cgtactgttt caggatacct cactggtgta tgtgttatct
    11881 ctggccgatt tcttccgtac cgcctcaacc attggtgagc gtgatggtac gcaggttgag
    11941 atgatcctgt ttgccggatt tgtttatttc gttattagcc ttagcgcgtc gttgttggtc
    12001 agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg
    12061 gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gttgtggtgg
    12121 tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac
    12181 cggtgcagca aggtgaaatc accgtcgatg gcatcgtggt taacgacaag aaaaccgatc
    12241 tggcgaaact gcgttcccgc gtcgggatgg tgtttcagca tttcgagctg ttcccccatc
    12301 tgtcgattat cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc aataaagcgc
    12361 cggctcgtga aaaagcattg aaattgctgg agcgcgtcgg gctttccgca catgccaata
    12421 agtttccggc gcagctttcc ggcggccagc agcagcgtgt ggcgatcgct cgcgcattgt
    12481 gcatggatcc tattgccatg ctgttcgacg aaccaacgtc ggcgctggat ccggagatga
    12541 tcaacgaagt gctggacgtg atggtcgaac tggcgaacga aggaatgacg atgatggtgg
    12601 tgacccacga aatgggcttt gcccgtaaag tggcgaatcg ggtgatcttt atggacgagg
    12661 gtaaaattat cgaagactcg ccgaaagacg ctttcttcga taatccgaaa tcggaccgcg
    12721 caaaagactt cctcgcaaaa atcctgcatt aatcactctg gcgcgcatac tcaattgcgc
    12781 gccatctcac gctttctcgt ttcgtccctc gtatctgtta catcccggcg ttacccttgt
    12841 cgcaaagaaa cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc
    12901 caggtcatga cgacgctatc gcaatagttc tcgccctcgc ctcaccagag cttgatgtca
    12961 aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc
    13021 gtatgctgac cttgcttaat cgcaccgata ttccggtagc aagcggtgcg gtaaaaccgt
    13081 taatgcgtaa tttgattatt gcggacaacg tgcacggcga aagcggcctg gacggcccgg
    13141 cattgccgga accggcattt gcaccgcaaa actgcacggc agtggagctg atggcgaaaa
    13201 cgctgcgtga aagtgcggaa cctgtcacca ttgtttctac cggaccgcaa actaacgttg
    13261 ccttgctgct caatagccac ccggaactgc atagcaaaat tgcccgtatc gtgattatgg
    13321 gcggcgcaat ggggcttggt aactggacgc ctgcggctga atttaatatt tacgttgacc
    13381 cggaagcggc agaaattgtc ttccagtcag gaattccggt ggtgatggcc ggtctggatg
    13441 ttactcacaa agcacaaatc cacgttgaag acactgagcg tttccgcgcg attggcaacc
    13501 ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcac aaagacgaaa
    13561 aatggggctt tgtcggcgca ccactgcatg acccatgcac catcgcctgg ctgttgaaac
    13621 cggagttgtt taccactgtt gagcgctggg ttggcgtgga aacacaaggg aaatataccc
    13681 agggtatgac agttgttgat tattattatc tgactggcaa taaaccgaat gccaccgtaa
    13741 tggtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg
    13801 cttaacatac gtcattgatt aagatcacca cgcattacct gggtggtctt aatctggtta
    13861 tctctttttc tttcaagata taaatgcaat ggataatgca gaacttgcta ttggtatcga
    13921 tctcggtact accaatagtt taattgccgt ctggaaagac ggtgccgcgc aattaattcc
    13981 aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa ataatcatat
    14041 tttagtcgga aaaccggctg tatcacggcg gacttcgcat ccggataaaa cggcagcgtt
    14101 atttaaacgt gcaatgggca gtaataccaa ctggcggtta ggcagcgaca catttaacgc
    14161 gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag aatttctgca
    14221 acgtccgatt aaagatgtgg tgatctccgt tcctgcttat ttcagcgatg aacaacgcaa
    14281 gcatacccgt ttagcagcag agttagccgg gctaaatgca gtgcgcctga ttaatgagcc
    14341 aacggcagcc gcgatggcgt atggcctgca tacgcagcaa aatacccgct cgctggtttt
    14401 tgatctcggt ggcggcacgt tcgacgttac ggtgctcgag tacgccacgc cggtgattga
    14461 agttaacgcc tccgctggcg ataacttcct cggtggcgaa gattttaccc atatgctggt
    14521 cgatgaggtt ttaaaacgcg cggaagttgc caaaaccacg cttaacgata gcgaactggc
    14581 agccttgtac gcctgcgtgg aagcggcaaa atgtagcaat caatcgccaa tgcacattcg
    14641 ctggcagtac caggatgaaa tgcggaaatg cgaattttac gagaacgaac tggaagattt
    14701 gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc gcgatgcacg
    14761 tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac aaatgccgct
    14821 ggtgcagcga atcgcagtgc gtctgtttgg caaattaccg tatcaaagtt acgatccgag
    14881 caccattgtc gcgctgggcg cagcaatcca ggccgcctgc cgcttacgca atgaagatat
    14941 tgaagaagtg atcctcactg atatttgccc ttactcgttg ggtgttgaag ttaaccgtca
    15001 gggcgtatcc ggcattttct cgccgattat tgaacgaaac accacagtac cggtgtcgcg
    15061 tgtagaaact tattcaacca tgcacccgga acaggattca attacggtta acgtctatca
    15121 gggagaaaac cacaaagtta aaaataacat tcttgtggaa tccttcgatg tgccgttgaa
    15181 gaaaaccggg acttatcagt cgattgatat tcgctttagt tatgatatca acggcttgct
    15241 tgaagttgac gtgcttctgg aagacggcag cgttaagtcc agagtgatta atcacagtcc
    15301 ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg cattgaaaat
    15361 ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc aaactggaag agttgtgggc
    15421 gcgggcgctg ggtgacgagc gagaagagat tggtcgggtg atcaccgatt ttgatgcggc
    15481 gttgcagtca aacgatatgg cccgcgtgga tgaagttcgg cggcgggcga gcgattattt
    15541 agccattgag atcccataaa cacggctacc ggatgcggcg taaacgctgc atccggcatc
    15601 ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt ttgccgagag cataacctat
    15661 caccagcacc gttaaactga ttttgataat gctggtaatg ctctgtggtt tttcatcctc
    15721 atcatttatt tcttcatggt tttcaggtgt tggctcatcg acatcttcat cctccggctc
    15781 gagcttcact ttcgtcaaca gcattcgggt gataatggtc tctaatggat gtgcctctcc
    15841 gcgttccagc cgcgcgtcca gacgggccag catctcttgt ttcggaaacg cttcaagtac
    15901 tacctgttcc gctacccgat acaaatccag taacggtgaa tcttgctctg tctcgctacc
    15961 gactctcgat ttccaccacg cccatgcttt gcgtttgaag aataacgact caataaacga
    16021 ttgacctaac ggcgataatt ttccgctatg taaaatctcc gcccataaca gccgcgtccg
    16081 ggcgctctca ccaggaacat atttcaacgg cttaccttgc gcatctacca tgctccgcgc
    16141 ttcgataacc atcactaata acgcgaaata atccgggcaa tgacgcttgc tccactgcaa
    16201 caacaaatta tcggcttgtg tagatgggta actttcccgg tacgcgcaga gatcggcaag
    16261 caggctttcc ccctcgccgc agtacaaccg ttgtgcgcac tggtaataac gggcatcttc
    16321 gctttctggt tcttccgctt gccactgttg agcgacagga atgagttccg ccatacctgt
    16381 ttgcaccgag ctgtaccagc gcaacagttt gcgatgcaac ttcgcatcat caggaatgaa
    16441 ccacggacct tccatcgcca aaaacgcagt gacatagtca ggatggtaac ggcagcaacg
    16501 ttccagcgag aagtaatact caaccgtctg gttctgcaac gcaacgggat attccagaag
    16561 gctaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct ccctttcttc
    16621 ctcatctatt ccttcgcagt cattaaacga atgccagttc aaacgctctg ccagcaaaga
    16681 agcacaattt aatgaaagat atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa
    16741 ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct gccaggcgga
    16801 gggctgaaaa cgatcatttt ctgaatccag taatgccctg aatacacgta gtatttcatg
    16861 ctcagtggca gcatcttttt cttcatcatc tgcttcctcg acaggattta ccgccagtcg
    16921 cagcgcctct tcataggcct ggcgtaatgc tttaaaccct tgtggatcgg tttcgggatg
    16981 acataacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg tcgtgctttc
    17041 aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag cgttaccggg
    17101 cattcaggct ggacagttaa tggctgccag ggaagcgtgg catagaaacg gtcgcttccc
    17161 gctgccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag ggttagcgcc
    17221 tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc agtagagacg ccctaacgaa
    17281 taggcagaaa aatagtgcat ccagctgtga tagtaatggt gtgctcgtat atagacgcgc
    17341 gattgcagcc ataaactctc gtcttccgtc aaccacttgt tgagcacacc aacccgtgat
    17401 aaaaaaccca tccgcacata atcccaggct ttgattcccc cttccccaca acacatagcg
    17461 gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccgccagc aattaatgct
    17521 ttccactctt caggggaaga gcgatgccat gtcagatata agccagctaa atcattggca
    17581 tgaccatcat cggtcatctg ataaatagtc tgaattaaat catcacgatt atttattccc
    17641 caggatgatg agagatcgaa ttcttttatc ccggtataca tttttggcga gtcatgcgcg
    17701 ggcttgcaac cctgattaag tatttccatg ggcgtagaaa gcgcaaaaag aagatattgt
    17761 tcttccttat tcatttgtta ataatccctt aagtgcatta actgactact tttttattat
    17821 tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa tcgaagcatt cacacatgaa
    17881 taaaaaatta atgtatatat tcgcaatatt tatagttgca gcaataacct gtattagcca
    17941 acccaagaaa acgacgttgc gtgataaagc catggcgaat tatgcctttg attatttaag
    18001 ctcaccgggc agtcttccat tcaccacggc agccacggag ctttccgcga ttcatggtca
    18061 ctcaacgtcg caatatcgcc ttggagaatt ttatcttcat ggtagcgacg gtaaaccact
    18121 ggattataca caggcgagat actggtatga gcaatcagca gaacaggaaa atccacgcgc
    18181 gcaaagtaaa ctggggtgga tctacctcaa aggtctgggg gtcaaacccg acacccgtaa
    18241 agcaattctc tggtataagg aagcagctga acaagggtat gctcatgctc aatatacttt
    18301 gggtttgatc tacagaaatg gctcaggtat taatgttaac cattatgaat ctcaaaaatg
    18361 gttaaaactg gccgccaaac aacattacaa aaatgcggaa agattacttg ccgggctttc
    18421 cgcacattaa cgtcgactgt cacccgaaca gatggagtat tttccctatc aaaccagcaa
    18481 agataaaaat atagaaaata attttaatga tgcccagaca gcctggccca ccatcgtcaa
    18541 cgggcattgg ctgcggttgc ggctccgata acgctggcga aggggtggac caggactata
    18601 agcaagcgat gtactggtat ctgaaggctg ctgctcagga aagcgtcggc gcttacgtca
    18661 acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag gccgcctttg
    18721 aatagtttac gatagccgct gaatgcaatg acgccacagc ctggtataac ctggccatta
    18781 tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac ttgtatcgta
    18841 aagttcagtc atccggaacc agagatgtca gtcaagaaat tcgtgagatt gaagatttac
    18901 tgtagagata aaaacaaggg ccggggaatt tcattcggcc catattctct ttattgctgt
    18961 gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcatg accacattta
    19021 gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag
    19081 accagatttc ccagccctac cacttctccg ctgtgataaa ccccatgatg attgaggtcc
    19141 tggaaaactt cgcgccattc aagctgcgtt ttatcggtga tgtctgccag ctcctgccac
    19201 aagctttctt taatcacccg cataaagacg ctgtcagact cttctttcag gctctcttca
    19261 tagctcatgg cgaactcttc caggtcacgt ctgacagctc gcgtcagctc atcgacctcg
    19321 gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg
    19381 atatcacgtt cgccattgcg caggcgttcg ctcagtgacg cgaccagttc acggtaatat
    19441 tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt
    19501 tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt atgaataatc
    19561 cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcgggcg tttgggctat
    19621 gctatgcgga tctgaaaaac cacatcaacg ctacatttgt agccgtattg aaaacaggac
    19681 cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc
    19741 actgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact
    19801 gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgtt cgcaactaca
    19861 ccatcggtga cgtgatcgcc cgctaccagc gcatgctggg caaaaacgtt ctgcagccaa
    19921 tcggctggga cgcatttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgcgc
    19981 cagcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct
    20041 ttggttatga ctggagccgc gagctggcga cctgtacgcc ggaatactac cgttgggaac
    20101 agaaattctt caccgagctg tataaaaaag gcctggtata taagaagact tctgcggtca
    20161 actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct
    20221 ggcgctgcga taccaaagtt gagcgtaaag agatcccgca gtggtttatc aaaatcacag
    20281 cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta
    20341 aaacaatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggaaatcacc ttcaacgtta
    20401 acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt atgggttgta
    20461 cctacctggc ggtggctgcg ggtcatccgc tggcacagaa agcggcggaa aataatcctg
    20521 aactggccgc ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga
    20581 cgatggagaa aaaaggcgtc gatactggct ttaaagcggt tcacccatta acgggcgaag
    20641 aaattcccgt ttgggcagca aacttcgtat tgatggaata cggcacgggc gcagttatgg
    20701 cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca
    20761 aaccggttat cctggcagct gacggctctg agccagatct ttctcagcaa gccctgactg
    20821 aaaaaggcgt gctgttcaac tctggcgagt ttaatggtct tgaccatgaa gcggccttca
    20881 acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc
    20941 tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc gccgattccg atggtgacgc
    21001 tggaagacgg taccgtaatg ccgaccccgg acgaccagct gccggtgatc ctgccggaag
    21061 atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta
    21121 ccgttaacgg tatgccagca ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct
    21181 cctggtacta tgcgcgctac acttgcccgg agtacaaaga aggtatgctg gattccaaag
    21241 cggctaacta ttggctgccg gtggatattt acattggtgg tatcgaacac gccattatgc
    21301 acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg
    21361 acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg
    21421 ttggtgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg
    21481 agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca
    21541 tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt
    21601 acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg
    21661 aatggcagga atccggcgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg
    21721 tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa cgtcgatgcg ctgactgaag
    21781 atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc taaagtaacc gatgatatcg
    21841 gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg
    21901 cgaaagcacc gaccgatggc gagcaggatc gcgccctgat gcaggaagcg ctgctggcgg
    21961 tagtccgtat gcttaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactga
    22021 aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg
    22081 aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgcaaaa atcaccgttc
    22141 cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa
    22201 aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa ctcctcaatc
    22261 tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt tgttatctct
    22321 ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc aggttccttc
    22381 cactatgaaa gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcggt
    22441 gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga
    22501 cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg
    22561 taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc
    22621 cggccgtgac atctacccga ttagcgccaa ggtcttccgt tcgttcttcg ataacccgca
    22681 aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc
    22741 tgccgaacag ctgattcgta agctgccaag catccgtgct gcagatattc gttccgacga
    22801 agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccacgct
    22861 gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg
    22921 cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct
    22981 gttcgtcagg tcgctgcggc gcaagggttc gaagaacacc acactttttc cattgatccc
    23041 aacaccgact ggaatgcgat tttttcgtta tgccaggcca tgagcctgtt tgccagtcga
    23101 caaacgctat tgctgttgtt acccgaaaac ggaccgaatg cggcgatcaa tgaacaactt
    23161 ctcacactca ccggacttct gcatgacgat ctactgttga tcgtccgcgg taataaatta
    23221 agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg
    23281 acctgtcaga caccggagca ggctcagctg ccccgctggg ttgctgcgcg cgcaaaacag
    23341 ctcaacttag aactggatga cgctgcaaat caggtgctct gctactgtta tgaaggtaac
    23401 ctgctggcgc tggcccaggc actggagcgt ttatcgctac tctggccgga cggcaaactg
    23461 acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg
    23521 gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca gcaactgcgt
    23581 ctggaaggca gcgaaccggt tattttgttg cgcacattac aacgtgaact gttgttactg
    23641 gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg
    23701 gtatggcaga accgccgggg catgatgagc gaagcgttaa atcgcttaag ccagccgcag
    23761 ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt
    23821 cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa acccctggcg
    23881 gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga
    23941 tccggtgcac tatggtcatc ttaaacccgt ggaaacgctg gcgaatttga ttggtctgac
    24001 gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag
    24061 catgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga
    24121 tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg
    24181 gcaggaacaa ggaccggacg tgccgctggc gtttattatt ggtcaggatt cattgctgac
    24241 ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg
    24301 gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca
    24361 tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga
    24421 aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc
    24481 gtgtgaggat ttattaccgg aaccggtact gacttacatt aaccaacaag gcttgtatcg
    24541 ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca tggtgaaacg
    24601 caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac cgcgcgcggt
    24661 attgagcaag cgcaaaatct gcatacgctg ctacatgatg tttcctttga cctggtttta
    24721 tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttcttagtga ccgccagcac
    24781 cccgttcata tcatccctga actcaatgaa atgttttttg gcgactggga gatgcgacat
    24841 catcgtgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag
    24901 caagctatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaacgattt
    24961 atcgcaaggc ttagtgaata tcagcactat cagaatattt tagtcgtcag ccatcagggc
    25021 gtactgagtc tgttaattgc tcgtttaatt ggtatgcctg ccgagtctat gtggcatttc
    25081 cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc
    25141 gtcctcaata gccgtgccat cggggtcgag aatgcatgac ttttctgttt ttttacgggt
    25201 aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc cagacttttt
    25261 ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg
    25321 gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct
    25381 ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt
    25441 gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga
    25501 catcatcgcc ttagacgttc agggcaaatc cagcatcacc gactgcatga tcatctgtac
    25561 gggtacgtcc agccgccatg ttatgtccat tgctgatcac gttgtgcagg agtctcgcgc
    25621 agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga
    25681 tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga
    25741 aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga
    25801 ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga
    25861 gctgattgaa attccggccg gaaaacgcgg caagaatgcg gacatcaagc gcatactcga
    25921 caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat
    25981 tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga
    26041 tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc
    26101 ggcggcggag cagagctggt cgctgtcggc acttaccctt ccccatccgc tggttcgcgt
    26161 gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg
    26221 tgagtgataa gtgagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg
    26281 cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat
    26341 tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac
    26401 cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg cacccagccg
    26461 cggcattatc tacgaccgta acggtatccc tctggctctc aaccgcacta tctaccagat
    26521 agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt
    26581 ggttgatctg accgatgatg atattgctgc attccgaaaa gagcgcgcac gttcacaccg
    26641 tttcacctct attccggtga aaactaacct gaccgaagta caagtagctc gctttaccgt
    26701 caaccagtac cgttttccgg gtgttgaagt taaaggctat aaacgtcgtt actatcctta
    26761 cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga
    26821 acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata tcggtaagct
    26881 gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga
    26941 agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aggccggaca
    27001 cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg
    27061 tagccgcgca gctgtggtag tcaccgatcc gcgtaccggt ggggtgttgg cgctggtttc
    27121 cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc
    27181 cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc
    27241 cgcgtctaca gttaaaccct atgtggcggt ttcggcattg agcgccggag tgatcacgcg
    27301 caatacgacg ttgtttgacc ccggctggtg gcaacttcca ggttcggaaa aacgttatcg
    27361 tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc
    27421 ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat
    27481 gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa
    27541 catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac
    27601 cattccggtt ggtatcggtc agggttactg gacagcgacc ccaatccaga tgagtaaggc
    27661 actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga tgagcaccgc
    27721 cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg gcgatattca
    27781 ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaacgg
    27841 tacggcgcat aaatattttg ccagcgcacc gtacaaaatt gcggcgaaat ccggtaccgc
    27901 tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt
    27961 acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc
    28021 catgattctg gagaacggtg gcgcgggtcc ggcggttggt acgctgatgc gccagatcct
    28081 cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc cagcggttgc
    28141 cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa
    28201 gtccatctcg atcccacaat gctgctgatc ttactggcat tgctggttta cagcgccctg
    28261 gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat cggccagatt
    28321 gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc
    28381 tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga tgctttcggt
    28441 gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt tcaaccgtcg
    28501 gaaattgcca agatagccgt accgttgatg gttgcacgct ttatcaaccg cgacgtttgc
    28561 ccaccttcat tgaagaatac agctatcgcg ctggtgctga tatttatgcc cacgctgctg
    28621 gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg cctgtttgta
    28681 ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtagcggcg
    28741 ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg
    28801 ctcctggacc cggaatcaga cccactcggc gcaggctatc acattattca gtctaaaatt
    28861 gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca gtcacagctt
    28921 gaatttctcc ccgaacgcca taccgacttt atcttcgcgg tactggcgga agagctggga
    28981 ttagtgggca ttttgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg
    29041 atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggcggctt aatgctgata
    29101 ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg
    29161 gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat ggctgggttc
    29221 gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg
    29281 cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac
    29341 aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc
    29401 catagttgaa attagcgggg cagacccgcg tttcgaacca ctgaacgcga cggcaaatca
    29461 ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca
    29521 ggcgggactg gcggcaatct atgatgccga acccggcagt aacctgacgg cctctggcga
    29581 agctttcgat ccgacacagt tgacggcggc ccatccaacg cttccgatcc ccagctacgc
    29641 cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc
    29701 ttatggcaac gaccgcgtga tttcgctttc tcgcgcggca gctgaccgtc ttaacacgtc
    29761 aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg
    29821 tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgccag cacctcccga
    29881 tttaagcggt ggcgcgggaa caagttcagt gcctgtcccg cagggtgaca ttcttccggt
    29941 cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gcagcggttt
    30001 cctcggcgca ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc
    30061 gccacagccc gttgttacag ctccgtcgac aacgcctgca acctcacctg caatggtgac
    30121 accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgttag
    30181 cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg
    30241 tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg ccagcaaagc
    30301 cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac
    30361 caccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca
    30421 aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt
    30481 ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc gctcgtatca
    30541 tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct gcacatgccg
    30601 atgacctgaa tatcaaaacc atgatcccgg gtgtaccgca gatcgacgcg gagtcctaca
    30661 tcctgattga ctataactcc ggcaaagtac tcgccgaaca gaacgctgat gtccgccgcg
    30721 atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca atgaaagccg
    30781 gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc accggtaacc
    30841 cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc
    30901 agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt
    30961 ttgccgctgg tagccaggac gcttttgttg gcttgatgaa cagctacgtt aacgccctgg
    31021 gcctgaaaaa tacccacttc cagacggtac acggtctgga tgctgatggt cagtacagct
    31081 ccgcgcgaga tatggcgctg atcggccagg cgttgatccg tgacgtaccg aatgaatact
    31141 cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac cgtaacggcc
    31201 tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact gacaaagcag
    31261 gttacaacct tgttgcttct gcgactgaag gccagatgcg cttgatctct gcggtgatgg
    31321 gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa attgctaacc tggggcttcc
    31381 gtttcttcga aaccgttaat cctctgaaag taggtaaaga gttcgcctct gaaccggttt
    31441 ggtttggtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac ctgaccattc
    31501 cgcgtggtcg catgaaagat ctgaaagcta gctatgtgct gaacagcagt gaattgcatg
    31561 cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgac ggcaaaacga
    31621 tcgaacaacg cccgctggtt gtgctgcaag aaatcccgga aggtaacttc ttcggcaaaa
    31681 tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg aacacttgaa
    31741 agtgtaattt ccgtccccat atactaag
//
LOCUS       DAOFSV010000041        30590 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.041, whole genome shotgun sequence.
ACCESSION   DAOFSV010000041 DAOFSV010000000
VERSION     DAOFSV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.041"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            101..715
                     /gene="evgA"
                     /locus_tag="QBH27_002027"
     CDS             101..715
                     /gene="evgA"
                     /locus_tag="QBH27_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDQ7014338.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            720..4313
                     /gene="evgS"
                     /locus_tag="QBH27_002028"
     CDS             720..4313
                     /gene="evgS"
                     /locus_tag="QBH27_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDQ7014339.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRKIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FAAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4369..5514)
                     /gene="yfdE"
                     /locus_tag="QBH27_002029"
     CDS             complement(4369..5514)
                     /gene="yfdE"
                     /locus_tag="QBH27_002029"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDQ7014340.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5588..6532)
                     /gene="yfdV"
                     /locus_tag="QBH27_002030"
     CDS             complement(5588..6532)
                     /gene="yfdV"
                     /locus_tag="QBH27_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDQ7014341.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6602..8296)
                     /gene="oxc"
                     /locus_tag="QBH27_002031"
     CDS             complement(6602..8296)
                     /gene="oxc"
                     /locus_tag="QBH27_002031"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDQ7014342.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8350..9600)
                     /gene="frc"
                     /locus_tag="QBH27_002032"
     CDS             complement(8350..9600)
                     /gene="frc"
                     /locus_tag="QBH27_002032"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDQ7014343.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10112..10744)
                     /locus_tag="QBH27_002033"
     CDS             complement(10112..10744)
                     /locus_tag="QBH27_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDQ7014344.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKAGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11039..11314
                     /locus_tag="QBH27_002034"
     CDS             11039..11314
                     /locus_tag="QBH27_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ7014345.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
     gene            complement(11391..11633)
                     /locus_tag="QBH27_002035"
     CDS             complement(11391..11633)
                     /locus_tag="QBH27_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDQ7014346.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            11986..12906
                     /gene="lpxP"
                     /locus_tag="QBH27_002036"
     CDS             11986..12906
                     /gene="lpxP"
                     /locus_tag="QBH27_002036"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDQ7014347.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13263..13334
                     /gene="ypdK"
                     /locus_tag="QBH27_002037"
     CDS             13263..13334
                     /gene="ypdK"
                     /locus_tag="QBH27_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDQ7014348.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13399..14637)
                     /gene="alaC"
                     /locus_tag="QBH27_002038"
     CDS             complement(13399..14637)
                     /gene="alaC"
                     /locus_tag="QBH27_002038"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDQ7014349.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            15014..16711
                     /gene="ypdA"
                     /locus_tag="QBH27_002039"
     CDS             15014..16711
                     /gene="ypdA"
                     /locus_tag="QBH27_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDQ7014350.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            16726..17460
                     /gene="ypdB"
                     /locus_tag="QBH27_002040"
     CDS             16726..17460
                     /gene="ypdB"
                     /locus_tag="QBH27_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDQ7014351.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSCSKVKEFRQLMHL"
     gene            17473..18330
                     /locus_tag="QBH27_002041"
     CDS             17473..18330
                     /locus_tag="QBH27_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7014352.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVLLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQLLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18333..20828)
                     /gene="ptsP"
                     /locus_tag="QBH27_002042"
     CDS             complement(18333..20828)
                     /gene="ptsP"
                     /locus_tag="QBH27_002042"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ7014353.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKLTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(20853..21890)
                     /gene="ypdE"
                     /locus_tag="QBH27_002043"
     CDS             complement(20853..21890)
                     /gene="ypdE"
                     /locus_tag="QBH27_002043"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ7014354.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(21890..22975)
                     /gene="ypdF"
                     /locus_tag="QBH27_002044"
     CDS             complement(21890..22975)
                     /gene="ypdF"
                     /locus_tag="QBH27_002044"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ7014355.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAVPKTVLLTGEA"
     gene            complement(22990..24237)
                     /locus_tag="QBH27_002045"
     CDS             complement(22990..24237)
                     /locus_tag="QBH27_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDQ7014356.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(24259..24585)
                     /locus_tag="QBH27_002046"
     CDS             complement(24259..24585)
                     /locus_tag="QBH27_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDQ7014357.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(24804..25769)
                     /gene="glk"
                     /locus_tag="QBH27_002047"
     CDS             complement(24804..25769)
                     /gene="glk"
                     /locus_tag="QBH27_002047"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDQ7014358.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            25973..27229
                     /locus_tag="QBH27_002048"
     CDS             25973..27229
                     /locus_tag="QBH27_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDQ7014359.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            27344..27670
                     /locus_tag="QBH27_002049"
     CDS             27344..27670
                     /locus_tag="QBH27_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDQ7014360.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(27811..29049)
                     /locus_tag="QBH27_002050"
     CDS             complement(27811..29049)
                     /locus_tag="QBH27_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDQ7014361.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            29385..30587
                     /gene="nupC"
                     /locus_tag="QBH27_002051"
     CDS             29385..30587
                     /gene="nupC"
                     /locus_tag="QBH27_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDQ7014362.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         8002 a         7710 c         7092 g         7786 t
ORIGIN      
        1 agctcaaatt ttcttatttt tattgagaaa atgagatgat accttatgtc tgtattacta
       61 cagggagaag ggaaatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
      121 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
      181 cttagcagag ttgactgaag gcggaagcgc cgttcagcgg gtggaaacac ttaagcctga
      241 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
      301 gagaaagcgc caatatagcg gaattattat tattgtctcc gctaaaaatg accattttta
      361 cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa
      421 caatatcatt gcagctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
      481 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
      541 agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca ttgctgaaaa
      601 aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt cgcttgatgg aaaaattaga
      661 atgcaaatca ctgatggatc tttacacatt cgcacaacgt aacaaaatcg gctaaccaca
      721 tgaagttttt accctatatt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
      781 cagacggaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc
      841 cactacgtct gagcaacaag gaattacgtt ggctagcgag caaaaaaaat cttgtgattg
      901 cagtacacaa gtcccaaaca gctacgttgt tgcataccga ttcgcagcaa cggattcgtg
      961 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc
     1021 gggaatacgc agatcatcaa aaagcaatgg acgcgctgga agacggtgaa gttgatatag
     1081 tgttatcaca tttagttgct tcgccgcctc ttaatgatga cattgctgca actaatcctc
     1141 tgataattac ctttccggcg ctggtcacta cccttcacga ttcaatgcga ccgcttacct
     1201 catcaaaacc agtaaatatt gctcgagtag caaattatcc cccagacgag gtaattcatc
     1261 aatcatttcc aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg
     1321 tctcagccgg acagaatgat tactttattg gtagtaacat cattaccagc agtatgattt
     1381 cccgttattt tactcactcc ttaaatgtag tgaagtatta taactcaccg cgtcaatata
     1441 actttttatt aaccagaaaa gattcaatcg ttcttaatga agtactcaat cgatttgttg
     1501 atgctttaac aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc
     1561 tggcctttct gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcagc
     1621 atcccgattt aaaggtgctg gaaaatcctt actcgccacc ctattctatg acagatgaaa
     1681 ctggctcagt tcggggcgtg atgggtgata ttcttaatat tattaccttg caaacaggtt
     1741 taaatttttc tccgatcacc gtttcacaca atattcatgc tgggacacag cttaaccccg
     1801 gtggatggga tatattgccc gccgctattt atagtgaaga tcgagaaaat aatgtttcat
     1861 ttgctgaagt cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg
     1921 aacaaacatt aaaaaaagga atgaaagttg ccattccata ttattatgag cttcattcgc
     1981 aattaaaaga gatgtatccg gaggtagagt ggataaaagt cgataacgcc agcgctgcat
     2041 ttcacaaggt caaggaaggt gaacttgatg ctctggtcgc gacacagtta aattcgcgtt
     2101 acatgatcga ccattactat cctaatgaac tttatcattt tcttattccc ggcgttcaga
     2161 atgcatcact ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata
     2221 aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct gaccgaaaaa tggattaaaa
     2281 tgcccaatgt gaccattgac acatgggacc tctatagcga gcaattttat attgttacga
     2341 cattatccgt tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag
     2401 ttcgtcgtcg taaagttatt cagggtgatt tagaaaacca aatatcattc cggaaagcgc
     2461 tctcggattc cttacctaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc
     2521 acaatagtgc atttgaacat tatttcactg ctgattacta taaaaatgca atgttaccat
     2581 tagaaaacag tgaatccccc tttaaagatg ttttttctaa tacgcatgaa gtcacagcag
     2641 aaacgaaaga aaaccgaaaa atatacacac aggtatttga aattgataat ggcatcgaga
     2701 aaagatgcat taatcactgg cataccttat gtaatctgcc agcaagcgaa catgctgttt
     2761 atatttgtgg ttggcaagat attaccgaga cgcgtgattt aattcatgca ctcgaagtag
     2821 agagaaataa agcgatcaat gcaactgtcg caaaaagcca gtttctggca acaatgagtc
     2881 acgaaataag aacaccaata agctccatta tgggcttcct ggaacttctg tcgggttctg
     2941 gtcttagcaa ggaacaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac
     3001 tcctcggctt aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac
     3061 ttcaaccaca atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcgctg
     3121 cgattgctgc aagcaaatcg atcgcattaa gttgcagcag tacgcttcct gaacgctacc
     3181 tggttaagat cgaccctcag gcgtttaagc aggtcttatc taatttactg agtaatgctc
     3241 tcaaatttac taccgagggg gcagtaaaaa ttacgacctc cctgggtcac attgatgaca
     3301 accacgctgt tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac
     3361 aacaacaact gtttaaacgc tatagccaaa caagtgcagg tcgtcagcaa acaggttctg
     3421 gtttaggctt aatgatctgc aaagaattaa ttaaaaacat gcagggcgat ttgtcattag
     3481 aaagtcatcc aggcatagga acgatattta cgatcacaat cccggtagaa attacccaac
     3541 aagtggcggc tgtcgaggca aaagcagaac aacctataac actacctgaa aagttgagca
     3601 tattaatcgc ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat
     3661 taggatatga tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa atcagtatgc
     3721 aacattatga tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga
     3781 ctcgcaaact ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac
     3841 aggctaacga acgtgaaaaa gggttaaatt gcggcatgaa cttatgtttg ttcaaaccgt
     3901 tgactctgga tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac
     3961 ctcagtatcg ccaccttgat atcgaggccc tgaagaataa tacggcgaat gatctacaac
     4021 tgatgcagga gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt
     4081 ttcatgcact agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg
     4141 gtgcggctaa catcctgaat ttgcaaaagt tgattaatat tagccaccag ttagaaataa
     4201 cacctgtttc agatgacagt aagcctgaaa ttcttcagtt gctgaactct gtaaaagaac
     4261 acattgcaga gctggaccag gagattactg ttttctgtca gcaaaatgac taaatagcgg
     4321 ctcccacaat gttcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa
     4381 ctcctggcga atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg
     4441 gtccgcgcag ccgctgattt ttatcggatt acccggcatc attattcccc cggcttcaat
     4501 caacatattt ctcgcctgag tttgtggtaa attaatggcc tcagccacac ttaataacgg
     4561 cgcgacgggc acaccaactt catgtattct ggctaaccaa acttcagcta cctgcgtttt
     4621 taacgtccgc tcaatatatt ctttaagaat agcctggtta tgtacgcgta aaatattgct
     4681 gctaaatcgg ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa
     4741 aagcttgtca ttaccacaac aaatcgtaat cggcttatcc tgagtatcga aaacatcaaa
     4801 aggtgccatg tagggatggc gatttcccag gcgttgtggt gacttcccag tcgcgatata
     4861 tgccatcagt ccatgctcca gaaaactcag agtggcatca aacatcgcta tatcgacatg
     4921 cgcccctctc tggctctttt cgcggccata aagtgcactc actattccac tgaataaata
     4981 aacaccgcca cagagatccg caagagaggt accaacgcgc actggcggag catcagggta
     5041 tcctgtttcc atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc
     5101 ttttagcgga ccggtatgtc cgaaacctga cgatgaagca tatataaggc gcgggttgat
     5161 ttcttgtaac ctttcccatg aaaacccgag tttttccatt gtacctgggc gaaaattctc
     5221 agctaataca tcagcttgtt taagcatatt tataaatata cttttatcgt gatcattctt
     5281 taaatcaaga accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc
     5341 atccacatag ggaccaaatg tgcgggtatc atcaccatga cccggtggct caactttaat
     5401 tacccttgcg cccatattac aaagaagttg agttccgaaa ggcccattaa ggacatgcgt
     5461 catatcgata actaataagc cttcaaacgg ccctttgcat tcattatttg tcatttgctt
     5521 atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca
     5581 tgataaatta tgaaaccagt cgactgacat aaatccacaa aggagccgtg acgacaaaac
     5641 ccagtacgct cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg
     5701 caataatgat tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcaaat
     5761 gttcgctatt caaatgacat gccataccga cgagaagcag cgccagtggc atcagaatca
     5821 gcttcaggaa agtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag
     5881 tcaaccccgc agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg
     5941 ggtcccatgc tgccggaatt tttaccccaa ccagcaccag gatcgttgcc agaacaggtg
     6001 cccatactac tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac
     6061 catccgctcc tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa
     6121 caataataga aataattgct accactaaac ctgttgatac ggaatcacca taaataggat
     6181 cgagaactgc aaaccccagg aatccaatgg taggtgaacc tgcaattaat gcacatactg
     6241 ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaacc gaaccaggag aaaaagaaac
     6301 atccgacaat aaccacaagt gatacaaggg tcaggcgagt gtccgcaaaa atcatttccc
     6361 tgtttgccct ggtaatagaa acaaatagag ccgcaggaag agcgtagttt aataccagtt
     6421 tattaaatgc ccgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca
     6481 acagcatgat tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat
     6541 ttccgataag ggtcccctca ccatcgcata ataagttggc aagaggagac gatgtcagta
     6601 tttaattacc agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg
     6661 ctgctgggtc gatgaccaca ttaataatgg tcggtttgcg cgactgaatt ccggtggtta
     6721 aagcatgacg aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca
     6781 ttaatttgtc ataccttgca tggtgcaaca gatccgttgg cgatggtgca ccagcgccgc
     6841 tgaggtcaac accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg
     6901 gcaggttata tcgacaaatc gtttcaatct ccatcccact gaaaccaaaa gcactatcac
     6961 cttcaatagc gacaaccgga gaaccagagg tcacgctagc accgatggca tagcccatac
     7021 cgatgcccat gacaccccag gtgccacaat ccagacgacg acgtggttta tacatatcaa
     7081 taatatttcg tgcattatcc agggtatttg ccccttcatt aactaaataa atatcctggt
     7141 tctcgcgcaa aacatcgcgc acggcactca atgcattaaa gtaatttaat ggttgggtat
     7201 ctgtacttaa cttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat
     7261 cacgccacac cagtggagtc gtaaatgtgt tttgtttcaa ttctgccagc ataccttgca
     7321 tactggatgc aatatcgcca acgactggca cagcaatggg gcggttgctg tcaatttcct
     7381 gcggttcaat atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg
     7441 ccagtaacca attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa
     7501 acgaacgcgc agctgccgca gagagtggat gcgtatcttc gaggatccct ttcgccatag
     7561 acattggcag gaatggaatc tgggcacttt caataaattc acgaagctgt tcatcagatt
     7621 gtgaatacgc cgcgcctttg ccaagaataa ttaatggtcg ttcagctttt gctaaaagcg
     7681 aaattgcgct agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt
     7741 taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca
     7801 aatcaagata aacaccgcca ggacgacccg atactgagac ccggatagcg cgtgccaatg
     7861 caatgccaag atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg
     7921 cattcatttg gtccagctct tcataatcac cttgctggag atcgacgatc gcgcggtcgc
     7981 tggagccgct aatcatgatc atcggaaaac catttaccgt tgcattggcc aatgcggtca
     8041 aaccgttgag gaatcctggc gcagacactg tcaggcagat ccctggtttt tgggtaagaa
     8101 aaccgcttgc tgcagcggca taacctgccg actgctcatg acgaaaacca atataacgaa
     8161 tgccttccgc ctgggcatgg cgcgccatat ccgtcacagg aatacctaca acaccataaa
     8221 tagtgtcaat attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt
     8281 gaagttgatc tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc
     8341 ccgttaatat cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc
     8401 cagctcctgc aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg
     8461 cgtaaaggca gagaatttca ttggacaacc aactgtcaga tattttccac gcaacggttg
     8521 ttccacttcg acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat
     8581 acttaaaacc ggtgcgcaag gaatatcaaa ctgagtcaaa taggccacag cttcatgttt
     8641 atcaatagtg acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg
     8701 ggcatgggct gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca
     8761 ggtgttttcc cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc
     8821 ccagccttta catttcagga tccagccagg ctgaccacca ccacccgcat taccaccgcg
     8881 gggaactgca tcaccaaatg tgccattcgg atactgcggg tattcttcca gataacccaa
     8941 tttatcgaga cgctgctggt cgcgtaattt cacgcggcaa aggttcaaca cggcatcctg
     9001 cattgacatg gtcactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag
     9061 taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag
     9121 cggcggaccg tcccaaaaac ctgtagtaga tgccgcgcca cccgctgcct gagcaacgtt
     9181 ttcataggct tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa
     9241 aatcagacgt ggattgattt cttgaatatg ctcccaggtg aagcccatat gatcaatggc
     9301 ccctggatga aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc
     9361 tttgccttcc gctgttttgg tatttaattc aatagaacgt ttgttactgt taagcatggt
     9421 gaagtaaagc gcatcgatat caggaatatc tcgcagctgg tggcgcgtta cgtcaccaac
     9481 gccgggacgt tcaattttaa tgacgtcagc gccaaaccag gccagcattt gagtacaaga
     9541 tgggccagat tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat
     9601 agcaatacct attagttaat taaatatgtg ttaatttagc gtttatttaa ggcataacgc
     9661 ctctataaat aatctgcaaa atatcgtcgt agctgcactc gatattttta aattcctgta
     9721 tcaagtgctg aaaatcattt catcgctact tccaattcct ttctgcatgg taagtagtga
     9781 ggacgaaaag aagaatataa taaatattgg ttcgcggtaa tcagagagag ccgcgacatt
     9841 tgtgatgtta ttcttacaac tgtaagaagt gtcagtgatt tacaagtaag gttatgatta
     9901 cactatttaa agcgtaccat aaaatataac ataataaatg atttgtgata atatgggatt
     9961 attgcggttt actttgagga agataaaatg cgagtatata gaaccgcgag cgccaaataa
    10021 ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg agggagttaa
    10081 atcctttacc tcaatatatt gcctgtcagg tttagttcac aaacagtgct tcgctatcaa
    10141 cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat
    10201 caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttgagcggc atcagatatt
    10261 ggttttccat tacgccaaca ccggccaggc gcagctcttc cattgcgccc tttttatccc
    10321 ctttcgccat tttttcgttg gcaattttta tcgcggcttc tttttcgggt gtcgctacat
    10381 aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca ttgacgttgg
    10441 tctttttacc tgccttagcg aactttgccc attcggtact atcatctgac agcaaagcgg
    10501 aagcttcatt ggtcagttct tttgcctttt ctggatcgcc atgaaacaga gccaggcgcg
    10561 ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg
    10621 ttttctgggt ttgcttaact tgctgagttt gctgttgtgc cgctaccggt gcattatcag
    10681 cagcccatac agttgaagat gccagaattg ctgtgaccat cgtagccata attaaacgtt
    10741 tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat
    10801 gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacaa ctgtagttat
    10861 tatcgcaaag ccgcttttag tcttacagtt gtaggaatct gcttcctgtc gcagagaact
    10921 attcttatat accacaaaga tatgtcattt attatatcga tgtaaatact gattaattaa
    10981 atataaatac agccataaca tcgtaggttt tcagtgaacc atttccagga tattttctat
    11041 gaaagtaaac ttaatgcttt ttagcttatt tttatttgtc tctattatgg catgcaatgt
    11101 ttttgcattt tctatttctg gtggtggtag tgaggcgagc tataaagaga cagaaaaaac
    11161 gtcagtgatg acgacaacgc attctacaaa acttcagcca tcacaggcga ttttgtttaa
    11221 gatgagagaa gatgcaccac cattaaacct cacagaagac atcacgccta cttatccgac
    11281 aaaggcgaat tatctcattc atcctttgcg atagcgtctg tcacctggca ggtgaacaat
    11341 catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca ctatcgcctg
    11401 ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa cataaatatc
    11461 cccgtcacca gttcattacc gccagttaac cacatggtga agtaataaat gagtgcagcg
    11521 aggatcacca tgccgaagaa agaagagatc gccgatacca tgttcagtac ctgaccgatg
    11581 tatccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat catagctcaa
    11641 cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga caatccttaa
    11701 cctctggcaa tttttgctaa gcatcaatca aaacagcatg ttaaatgcaa gactgttacg
    11761 tacggaaaaa tatttacttt gcacgattaa taatcagtag ctgaaagcag tcagcgagtg
    11821 ataaagtaca gataccgcat tcatgctcct cgtggttatg tcctgactag tctttacact
    11881 ctttacagga accattgtcg tacatgatgg cctaatcaat tgagtgtacc gctgtcgaca
    11941 tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt tccaaaatgc
    12001 aaattttccc gcgagtttct gcatcctcgc tactggctca cgtggtttgg gcttggtgta
    12061 ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac gcgtatcggc
    12121 acaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt
    12181 tgtttcccgc agcattctgc ggaagagcgc gagaagatga ttgccgaaaa ctttcgttca
    12241 ctcggcatgg cgctggtaga aaccggcatg gcgtggttct ggcccgacag tcgcgtacgt
    12301 aaatggtttg atgtggaagg gctggataac cttaaacgcg cacaaatgca aaatcgcggc
    12361 gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggtcgggt gatgggactt
    12421 tgccaaccaa tgatggccac ctatcgcccg cataataatc agctgatgga atgggtccag
    12481 acccgtggtc gcatgcgctc taacaaagcg atgatcggca gaaataatct gcgcggcatt
    12541 gtcagtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga ttatggtcgt
    12601 aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac caatggcacc
    12661 tatgtcctct ctcgtctctc tggcgcagcc atgttgaccg taacgatggt aagaaaagcg
    12721 gattacagcg gatatcgttt gtacatcacc ccagagatgg aaggctaccc gacagatgaa
    12781 aatcaagccg ctgcctatat gaacaagatt atcgagaaag agatcatgcg cgcaccggag
    12841 cagtacctct ggatacaccg tcgctttaaa acgcgcccgg tgggtgaatc gtcgttatac
    12901 atttaacaaa cggctctcac tctttccctc ctcaaatcag ccataataac ctcatggttt
    12961 ttatggcttt ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa
    13021 attaaactgt cgccgatatg agacacctgt aagttactaa aagtaaataa gaaaatgcct
    13081 cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat
    13141 tctgggatga tgcccacggt aaaatacagg acgtttaagg tcgttttgcc cggtgtcaac
    13201 tcactgtatc aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta
    13261 aagtgaaata tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg
    13321 ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccattagg ctcctgtttg
    13381 catttctggc gaaggcgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag
    13441 taaaccgtcg gcacggaaca tcgctttaat cccacgaatc gcctgacgaa tacggtcgcg
    13501 gttttcaatc agtgcaaagc gcacatgcgt gtcgccgtag tcgccaaagc caatccctgg
    13561 agagacacag accttcgctt cgttaagcaa tttcttggca aattccagcg agcccatggc
    13621 agcatacggt tccgggattt tcgcccagac atacatcgaa gccttaggca tttcgaccat
    13681 ccagcccgct tcatgcagcc ctttcaccag cacatcgcgg cggcgtttgt actgttcggc
    13741 aatatcgcgc acgcactgtt gatcgccctc cagcgccgca atagccgcca cctgcaacgg
    13801 cgtaaaggtg ccgtaatcgt gatagctttt aatacgtgcc aacgcgctga ccagcgtttt
    13861 gttaccgacc ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa
    13921 gaactcgacc gccacgtcgc gtgcgcccgg cacctgcatg attgacggcg ctttccagcc
    13981 atcgtagacg atatcggcat aggccaggtc gtggaccacc agcacatcgt agcgtttagc
    14041 cagcgccaca accttctcaa agaattccag ttccacgcat tgcgcggttg ggtttgacgg
    14101 gaagccgagg atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc
    14161 attgaagaag tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac
    14221 cgcgccgtaa atatgaatgg ggtagcttgg attcggcacc agcactgtgt caccatgatc
    14281 cagcgtcgcc agcatcaggt gcgccagccc ctctttcgaa ccaatagtga cgatggcttc
    14341 tgattccggg tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg
    14401 taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccaccgt
    14461 acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tactgaaatc
    14521 gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtaa tgttaaaaac
    14581 gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat
    14641 ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacga tggtggctgt
    14701 tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatcaatgg
    14761 ctttaaatga ggaactccac ttcaaaaaca ggaaatttat ctacgctaac aagaaaatta
    14821 ccactcattt tattaacatt ataatttcaa taaatttacc aacaagatgt gcattatcaa
    14881 gagatgcatt gctaatacac catttatctt tcaatcccct tttaaaattg tggtttttcc
    14941 tcaaaaaagg cctgctggtc atcagccttc aggttaccta tcatagaggt ttaatcctta
    15001 ttcagagtca cccgtgcacg aaatattcaa catgctgctg gcggtcttcg atcgggcagc
    15061 gttaatgctt atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg aactgttgca
    15121 caagtcggcg cactccccaa aagagttgct cgccgttacc gccattttct cgctgttcgc
    15181 cctgttcagc acctggtccg gcgttcccgt agaaggctcg ctggtgaacg tacgtattat
    15241 cgcggtgatg tcaggcggga ttctgtttgg cccgtgggtg ggcatcatta ccggcgtgat
    15301 tgcgggtatt caccgttatt taattgacat cggcggcgtg acggcgatcc cctgctttat
    15361 caccagcatt ctggcgggtt gtatatcggg ctggatcaac ctgaaaatcc ccaaagcaca
    15421 gcgctggcgc gtcggtattc tcggcggcat gttgtgtgaa acgttgacca tgattctggt
    15481 gattgtctgg gcaccaacta ccgcattggg gatcgatatc gtctctaaaa tcgggattcc
    15541 aatgatcctc ggcagcgtct gtatcggctt tattgtgttg ctggtacaaa gtgttgaggg
    15601 agaaaaagag gccagcgccg cgcggcaggc caagctggcg ctggatatcg ccaacaaaac
    15661 gctgccgctg tttcgccatg tcaatagcga gtcattacgc aaggtctgcg aaattatccg
    15721 cgatgacatt cacgccgatg cggtggcgat gactaatacc gatcatgtgc tggcctacgt
    15781 tggcgtgggt gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca
    15841 ggcgatgaat tacgggaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga
    15901 gattcattcc atgctggtga tcccattgtg ggagaaaggc gttgtgaccg gaacgctgaa
    15961 aatttactac tgccacgcgc atcagataac ctcgtcatta caggaaatgg cggtcggtct
    16021 gtcacaaatc atctccacgc aacttgaggt gtcacgcgcc gagcagctac gtgaaatggc
    16081 aaataaggca gagcttcgcg ccctgcaaag caaaattaat ccccatttcc tgtttaacgc
    16141 tttgaacgct atttcatcgt caatccgtct gaatccggat actgctcgcc agctgatctt
    16201 taatttgtcg cgctatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat
    16261 caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggcga
    16321 caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct
    16381 gatccagccg ttggtggaaa acgccattgt ccacggtatc cagccttgca aaggtaaagg
    16441 cgttgtcacc atcagcgttg ctgagtgcgg taatcgggta cgcattgcgg tgcgagatac
    16501 cgggcacggc atcgatccaa aggtgattga gcgggtcgaa gcgaacgaaa tgccgggcaa
    16561 taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca
    16621 tatccgccgc ctggaaccag gaacggaaat tgcgttttac attcctaacc aacgcacccc
    16681 agtcgcctca caggctacct tattgctttg agccggagtg atattgtgaa agtcatcatt
    16741 gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag
    16801 atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac
    16861 cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggtgt gttgctggca
    16921 caaaacatca gccagttcgc ccataaaccg tttattgtgt ttatcaccgc gtggaaagaa
    16981 catgcggtag aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag
    17041 tcacgcatta ccgggatgct gcaaaaactg gaagcagcct ggcaacaaca gcagaccagc
    17101 agtacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag
    17161 cgaatcatcg tcacgcccat taacgatatc tattacgccg aagcgcatga gaaaatgacc
    17221 tttgtctata cgcggcgtga atcctacgta atgccgatga acattaccga gttttgcagc
    17281 aagctgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa
    17341 atccgcgaga tcgaaccgtg gtttaataac acctatattc tgcggctgaa agatctggat
    17401 tttgaagtgc cggtcagctg cagcaaagtt aaagaattcc gccagttaat gcatctttaa
    17461 tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg
    17521 ttcagcggcc tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct
    17581 acctcattgc cagtgggcag tttatgtatt gattttcccc gtctggatat cgtgctacgc
    17641 ggcgaatacg gcaatctgct ggaagcaaag cagcaacgta tggtggaagg agaaatgctg
    17701 tttattccag cacgcgcggc taatttaccg gtcaacaaca aaccggtgct gctgttaagc
    17761 ctggtgttcg caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg
    17821 ttacatcctg ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg
    17881 cttaccgccc tcacccatct tagccgttcg ccgctggagc agaatatcat tcagccactg
    17941 gtgttaagtt tgctgcatct ttgccgcaac gtggtgaata tgccgccggg caattcgcaa
    18001 ccgcgcggcg attttctcta tcacagcatt tgtaactgga ttcaggataa ttatgcccag
    18061 ctgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgccaaatca tctgtcaaaa
    18121 ctgtttgctc agcatggaac aatgcgtttt atcgagtatg ttcgctgggt acgaatggca
    18181 aaagcgagga tgattttgca aaaatatcat ctgtcgattc atgaagtggc gcagcgttgc
    18241 gggtttccgg atagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg
    18301 ggagagtata gcgcacgttt tcagggctaa cgtcagaagg ttaattccgt ttccagcagc
    18361 gtcagaatac tttgtgcatc ttgcgcggca aacagcgact ggcggaagtt tttattcacc
    18421 agtttacgcg ccagctgcga gaagactttc acatgattca tcccttcgtt agcgcccagc
    18481 gtcagcatga tcaccagttc gacttcgccc atttctgact gccagtcaac cggtttcacc
    18541 aaccgggcaa tgctgatact ggaatgacgg atccactgtg atttggtgtg cgggatcgcc
    18601 acgccaaaac caacgccagt agtaacaatc tcttcccgct gccagacatc ttcttccagc
    18661 tcgaacggat gttcagtacg cccgttaaca ccgaggttac cgcacaggaa ctggatcgcc
    18721 tgctctttat tactaaagga ctgatcaaca aagatattct ctagtgccag cagtgggcga
    18781 acgtcttctt ccggcgcaaa cgcggtaagt aacgcttcaa tttcctgcgc gctgcggcac
    18841 tcgcacgcct gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt
    18901 ttcactgccg gaatacgcgg gctactcatg cttagctcat ccagccccag ccctagcagt
    18961 agcggcagat aacgactttc accgcccagt tcaccgcaaa tacctaccca tttgccgcgc
    19021 tgatgcgcag cggtaacaat ctgctgcaac atgcgcagga acgatggcgt aatcgggtta
    19081 tacagcggcg atacgcgcgg gttattacga tcgactgcat acagatactg ggtcatatcg
    19141 ttagaaccga tactgaagaa atcgacctca tcgcagaagt ggtcgatgat gtagcacacc
    19201 gacggcactt ccaccatgat acccagcgta atcgtctctg catgacgcag gccatcgcgc
    19261 ttaagctcaa cgatcgcttt ttggatctca cccttcaccc ataagatctg atcgagactg
    19321 tggaccattg ggatcatcaa ctgggcgttg ccgaaactgg cagcgcgcaa aatcgcccgc
    19381 aattgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg
    19441 aacgggttct cttcctgggg aatgttgagg taaggaatgc ttttatcgcc accgatgtcc
    19501 atcgtgcgga agataatcgg cttgtcgccc gccgccaata gcacctgctg gtacgcttca
    19561 aactgttcct gctcgtccgg cgcgctgtcg cgatccatat agagcatttc ggtacgaaac
    19621 agcccaacgc cttccgcgcc gttagcaaac gcgcctgggg cttccagagc ggtgccaata
    19681 ttcgccgcaa tatcaatacg tttgttatca cgggaatagg ccagctgcgc ggcagcttgc
    19741 gcctgttgtt tttggcgttt atccgccagc gtctgcgcga cctgataata accgctcacc
    19801 gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt caagcactgc aggttgcccg
    19861 gcataacggg caatcgcatc cagcggcagt ccgctcagta ccgggatcgc cgaagcgcgg
    19921 gccaggatca gggtatgcga ggtgcgcccg gttttctcca gaatcatgcc cgcaagattt
    19981 tttaaatcga ggctcaaaaa ctggcttggg gttaaatctt cagccaccag aatggtcagt
    20041 ttttcaagca ccagattatt gcgcggtttt agttccggcc aggtgatatg cagcaactgt
    20101 tcgctgatat cgcgaatgtc gctgacccgc tcgcgcagat aatcgctggc agaagcagaa
    20161 agtttggcac aaacctgctc catattgctg atgatcgccg cccccagccc ctgatgctgt
    20221 tctgccatca ggtgacggat attgcctgca aattcatcat cctgaatcag cgacaaatgg
    20281 gcgctgagga tagttttgct ttcgccgtca cgctcacgca attgctggtt cagttgctcg
    20341 gcaagcgttg ccagactgtg ctccagtagg gtggaatctt gcgcactcgc agggatcacc
    20401 cgataactgt cgaggctgtc gctctgtaac agggtcagtg tacccacgcc aacgccgctt
    20461 gccagcacgt tgccgtacag taaatccggg ttcaggcggc ttaatgaacg cggcagcgga
    20521 tgcgccgtca gttctgccag cgtcggctgg acgctatcgc tgtcgataaa gcgcacctgg
    20581 atatactctt ccagcactcg ccgcgcctgc tcttcatcgc taccgctaat gttcaggctg
    20641 caactgtcat taaataaagt gccagtgcca atcagcgcca gcgagctttt cgcatcggcc
    20701 tttgcgttct ggcgatggtt aataaaagtg atttcgcttt gccactggct gcactgctct
    20761 ttaagttccc acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc
    20821 gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc
    20881 gtctcacgcg taagacgttg aataagggca gataaaagtt gctccatctg caaaatgtca
    20941 cggcagtcag caatcgatgc ggcgcaatgt ccgtggcggg ttgcaggccc catcaccact
    21001 gtgggtacgc cagtgccggt taagtgcacc gccccaccgt ccgtgccgcc attgctgaac
    21061 atatctgact gcaacggtac gccaatttct gccgccacgg tttcgatcca ggcggtaagt
    21121 tttggcggcg caatcagtga cttgtcgctt aacaccagca tcggcccgtt accaatctgg
    21181 cgatggttag ctgcgccata atcaaagttt ttcgcccagc aggcagtatc aagcacaatg
    21241 gcgacatccg gcgacaccgc gcgggtggca gtttgcccgc cgcgtaatcc cacctcttcg
    21301 ctggaacttg ccaccagcca cacttccgca ggcagttcgg cgtcgtgcag ctcacgcagt
    21361 aacgtcacca gcagatagca accgaggcgg tcatcaaagg ctttccccat cacccgctgg
    21421 tgagggagaa cctgaaaagt ggtatcgaag gtgacgcgat cgcctggacg aattcctgcc
    21481 tgcatcactt cgtcacagga gcgcgcgcca atgtccacgc gcatggcgct gacgtcattc
    21541 ccctgccggt cgctgtcaag caggcctgga attttgcact cttcacgggt agtgatacgc
    21601 accgactgaa gctggcgggc agccatgcgt acattgccaa ccggcaacac atcgatcgcc
    21661 ccttcgcggg agatgctgcg caccataaat cccacttcgt ccatatgcgc acagatcatc
    21721 accttcggac ctgtcgactc attgagtcgg atcagcaccg atcccaggcc gtcaaatcgc
    21781 acttcttttt gcaggcgatc cgcttcttcc cgcaggatct gccgtacttc ctgttctgag
    21841 gaggcgatcg catctgcctc gctcaacgct tttaagagcg ataaatccat tatgcctctc
    21901 ccgtgagcaa cactgttttc ggcacggcgt agagcacttc tgcgccttgc ggggtgacca
    21961 gcacgacatc ttcgatgcgc acgccccctt gccctggcaa ataaatccct ggctccacgg
    22021 tcagtaacat acctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggttcttcat
    22081 ggacttcaat accgatagca tgaccggtgt tatggccgaa aaacttgcca taaccggcct
    22141 cggtaatcac gcggcgcgcg gcatcgtcaa cctgctggca gcgcacgccg gggcgaatta
    22201 cagagattgc cgcgagctgt gcctgcagga caatctgata gacgttaaac agcggatgag
    22261 attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc
    22321 cctgatacag cgcaccgaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc
    22381 tggctttgcc gtgcggcagc gccccacgcc agccgctggc gacaatggtg tcaaaagagg
    22441 ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc tcgcattcgc
    22501 tcattcccgc ctgaataaag cggcgaatat gctctgcgcc acgatcggca atcccgcagg
    22561 caaggcggat tttctccacc tcttctggcg ttttgatttg ccgcagcaca tccggcgtgg
    22621 cgctcaccag tttcgcattg agttcggact gccagcggtg cgcggtttcc caactcacct
    22681 gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttgt ctgacgatgg
    22741 tggcaagcgt gtgcatcgcg tcgagcaaat gcagctggta accttgcgtg cgggcttcta
    22801 catccgcgta atagcgcgaa tccaccagaa tgtgcgcact ttcacggcta atcaccacat
    22861 aacctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca
    22921 ccgcatccag ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat gtcatcagga
    22981 ttgtccttct tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag
    23041 gaacaccacc atcagcgcag ttatgactgc ccccagcgcg atccccgcca tgtagacgcc
    23101 gaggttagtg accagcggcc atgcccagat agcggactcc gggaaccatt gcactgcgcc
    23161 cagccagaca gcggcggttg agccgacaat cgcgccgacc atatacgacg gaatggcggt
    23221 gatagggctt tccagtgcaa acggaatcgc accctcactg atccccataa acgccaggaa
    23281 catggcggtt ttaccctgcg gataaagctg ggcgttgaac aagcgtttgc cggttaaacg
    23341 acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag cgatagaacg
    23401 tgcagtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac ctgccgcttt
    23461 gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg cgtacatcag
    23521 tgcgcccttc tcacctgcag cggtcagcac ggtacggata ccgccgttga tccagccacc
    23581 aaagggggtg atgacgtagt acatcgccag catgacaaaa atggcggaaa gaatcggtag
    23641 caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct ggttcatcca
    23701 tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg tggacggcac
    23761 cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg
    23821 ggtagacatc aagccgccga taaaaccagc cgggaacgcc agtttgccac cgatagagtt
    23881 cgccacaaaa gcagcaaaca tcggaattgc aaaaccaaac aacacgccgc cgaaggactg
    23941 cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc cgctattaag
    24001 agcatccatg atgccgatat cagcaggaat tttaagccag ctataagcaa tcagctgact
    24061 gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat
    24121 cacatgctgc gggagttcac cccagaagct ggatttagag gcctgcggat tcttaactgc
    24181 cgctgccgca ccggatgcgc catgcacaac ggttgcactg cgttttttaa tggccataac
    24241 aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat
    24301 gattcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc
    24361 gaaacgctcg ttatcttccg gggtaactgc cacggagtgg atgatgatgg tcgcttcggc
    24421 gatatcctgc gccgtcaggc gattctggat accgtccgcg ccctgggttt caattttcac
    24481 ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa aagtgtgagc
    24541 caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcgtt
    24601 cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa
    24661 taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg tcggccttgt
    24721 gaggggacac aaagaaaaat ttaattaact gtatgattta aaagattatc gggagagtta
    24781 cctcccgata taacaggaag gatttagaga atgtgaccta aggtctggcg taaatgtgca
    24841 ccggaaccga gaagacccgg attgtcatgg acgatgagat ataccggaat atcatggaca
    24901 tattccttaa agcgcccttt atcttcaaat gcggcgcgga aaccagaggc tttgaagaac
    24961 tcaagaaagc gcggcacgat accacccgca ataaacacgc cgccaaatgt cccgagattg
    25021 agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcgcggcgg
    25081 caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt gagattttct
    25141 ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat tcaccagccc agggccagaa
    25201 agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca atatttcgag gataatgccc
    25261 tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt ctcctggcaa gcttacccaa
    25321 cgcttatcga catggaccag atgcgcaacc ccaagccctg ttccggcacc gtaaaccgca
    25381 ataggcttac cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc
    25441 atcgggatcg ccatcgacac agcagtaaaa tcgttaataa tttccagatg gctaaaaccg
    25501 agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag
    25561 tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct
    25621 tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta
    25681 gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc
    25741 acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat
    25801 tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct
    25861 gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt gcagcgatgc
    25921 ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca
    25981 tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc
    26041 cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca
    26101 acgactgccg ggaactctgg ggatagccca ggattcacct ctctggatca tcggtgtatt
    26161 aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc
    26221 agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc taccaggact
    26281 tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat
    26341 catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac cgcgcgtcaa
    26401 ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggcacacc
    26461 tgttgcggcg gcattgatat tttcgcaaac cttaaatggc agtaatgaag ttccgctatg
    26521 ggatcgtctc tttgcgccgt taatggcggc agcagctggt gcacttacta ccggattatt
    26581 tttccatcct catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat
    26641 tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg
    26701 cttaccacgg ttgcacgcga tgatgcatca aatgaaaaat ccggtgttcg tgctgggtat
    26761 tggcggattt attctcggta ttctgggggt tattggtgga ccggtttcgc tgtttaaagg
    26821 gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt
    26881 gctggcggtg ataaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg
    26941 gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt
    27001 acccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg tgctggtggt
    27061 aacacgtgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca ataccacatt
    27121 gctaccgctg ctctgtatcg tcatgctccc ggcatggctg ttattagcag gtaagccgat
    27181 gatgatggtc aatcgtccga agcaacagcc accccacgat aacgtttagc aaaaaatgct
    27241 ctcctttatg atttaagagt tatggcgatg atacggcgct ttacctcccc gtaatattgc
    27301 gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt
    27361 tctggcggcc gccctgatgg catttacccc gcttgcggca aacgcaggtg aaatcaccct
    27421 actgccatca atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg
    27481 tggtcactgg cgcgaccgtg actactggca ccgcaattat gaatggcgta aaaaccgctg
    27541 gtggcgtcat gataatggct accatcgtgg ctgggataag cgtaaagcgt atgagcgtgg
    27601 ctatcgtgaa ggctggcgcg atcgtgacga tcatcgtgga aaaggccgcg gacatggaca
    27661 ccgccattaa acacattcgc caatggagca caatgcctga tgcgacgcta gcgcgtctta
    27721 tcaggcctac aaaccagctc tttaacgcgt agggcggata aagcatttac gccgcatccg
    27781 ccaatggtgc caaatgcgac gctcattcaa ctacaatccc agtgccgttc ctaccagtaa
    27841 ccagatattc agcgccacta ccagcaccac aatcacccag cctgtctgtt ttacgcgttt
    27901 gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcaaca gtggaaccag
    27961 cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc
    28021 taatcccatc agaatgacaa taaatgacgg cagcatggtg acagtacgac gcacccacag
    28081 cgggatatga aagcgaatga agccctgcat cactacctgc cccgccagcg tccccaccac
    28141 cgttgaggac aacccggcag caaccaggct taatccaaag accgttgccg cagcatggct
    28201 taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga
    28261 aaagtggaac gccgccgcag ctgtagccat catcgccaga ttaacaaaac cggcaatcgt
    28321 catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg
    28381 taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa
    28441 cacgcccgcg gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt
    28501 acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag cagcggcaac
    28561 aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat
    28621 taaaataagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag
    28681 aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat
    28741 aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat cgcgaatctg
    28801 ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggattt gaatcagcat
    28861 cgccatcagg ttagcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc
    28921 ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg
    28981 tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata
    29041 gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta cgttaaatga
    29101 gaatgattat caaattcatt taaatgggtt gtgatgattt ctctgataga ccaggattat
    29161 gactacgaaa agatagatga ctcgaatgtt atggttatgt ttaatattag cacatttaca
    29221 taaccttccg cttccataca caacatagca gaaatgtatg acagatcact atttttgaag
    29281 cttgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat
    29341 agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc
    29401 attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca
    29461 aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt
    29521 tcttcctgaa ctccgacgtt ggtctgggct tcgtgaaagg cttctccgaa atgttcgaaa
    29581 aactgctcgg ttttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag
    29641 gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg
    29701 gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt ttcctgctct
    29761 ccaaagtcaa cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg
    29821 gtcagtctga aaactttatt gcctataaag atatcctcgg caaaatctcc cgcaatcgta
    29881 tgtacaccat ggcagcaacg gcgatgtcca ccgtgtcgat gtccatcgtt ggtgcataca
    29941 tgaccatgct ggagccgaaa tacgtcgttg cggcgctggt actgaacatg ttcagcacct
    30001 ttatcgtgct gtcgctgatc aacccttacc gtgttgatgc cagtgaagaa aatattcaga
    30061 tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag
    30121 gtttcaaggt tgccattatc gttgcggcga tgctgatcgg ctttatcgcc ctgatcgccg
    30181 cgctgaacgc actgtttgcc accgtgactg gctggtttgg ctacagcatc tccttccagg
    30241 gcatcctggg ttacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag
    30301 cactgcaagt gggcagtatc atggcgacca aactggtttc caacgaattc gttgcgatga
    30361 tggatctgca gaaaattgct tccacgctct ctccgcgtgc ggaaggcatc atctctgtgt
    30421 tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcgggtgcg gttaaaggcc
    30481 tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt tacggctcta
    30541 ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac
//
LOCUS       DAOFSV010000042        30587 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.042, whole genome shotgun sequence.
ACCESSION   DAOFSV010000042 DAOFSV010000000
VERSION     DAOFSV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.042"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            546..790
                     /locus_tag="QBH27_002052"
                     /pseudo
     CDS             546..790
                     /locus_tag="QBH27_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(807..1478)
                     /locus_tag="QBH27_002053"
     CDS             complement(807..1478)
                     /locus_tag="QBH27_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDQ7014363.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLTSIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            1625..1900
                     /locus_tag="QBH27_002054"
     CDS             1625..1900
                     /locus_tag="QBH27_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7014364.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2001..3587)
                     /gene="prpR"
                     /locus_tag="QBH27_002055"
     CDS             complement(2001..3587)
                     /gene="prpR"
                     /locus_tag="QBH27_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDQ7014365.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            3826..4716
                     /gene="prpB"
                     /locus_tag="QBH27_002056"
     CDS             3826..4716
                     /gene="prpB"
                     /locus_tag="QBH27_002056"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDQ7014366.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            4876..6045
                     /gene="prpC"
                     /locus_tag="QBH27_002057"
     CDS             4876..6045
                     /gene="prpC"
                     /locus_tag="QBH27_002057"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDQ7014367.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEIFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6079..7530
                     /gene="prpD"
                     /locus_tag="QBH27_002058"
     CDS             6079..7530
                     /gene="prpD"
                     /locus_tag="QBH27_002058"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDQ7014368.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7570..9456
                     /gene="prpE"
                     /locus_tag="QBH27_002059"
     CDS             7570..9456
                     /gene="prpE"
                     /locus_tag="QBH27_002059"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDQ7014369.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            9781..11040
                     /gene="codB"
                     /locus_tag="QBH27_002060"
     CDS             9781..11040
                     /gene="codB"
                     /locus_tag="QBH27_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDQ7014370.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            11030..12313
                     /gene="codA"
                     /locus_tag="QBH27_002061"
     CDS             11030..12313
                     /gene="codA"
                     /locus_tag="QBH27_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDQ7014371.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
     gene            complement(12434..13045)
                     /gene="lacA"
                     /locus_tag="QBH27_002062"
     CDS             complement(12434..13045)
                     /gene="lacA"
                     /locus_tag="QBH27_002062"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDQ7014372.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGCHVVINPGITIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(13111..14364)
                     /gene="lacY"
                     /locus_tag="QBH27_002063"
     CDS             complement(13111..14364)
                     /gene="lacY"
                     /locus_tag="QBH27_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDQ7014373.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSHFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLVAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(14416..17490)
                     /gene="lacZ"
                     /locus_tag="QBH27_002064"
     CDS             complement(14416..17490)
                     /gene="lacZ"
                     /locus_tag="QBH27_002064"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDQ7014374.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVKLHTADGTLIEAEACDVGFL
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFNRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(17613..18695)
                     /gene="lacI"
                     /locus_tag="QBH27_002065"
     CDS             complement(17613..18695)
                     /gene="lacI"
                     /locus_tag="QBH27_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDQ7014375.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(18772..19605)
                     /gene="mhpR"
                     /locus_tag="QBH27_002066"
     CDS             complement(18772..19605)
                     /gene="mhpR"
                     /locus_tag="QBH27_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDQ7014376.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLLEEGYVRRSTSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            19796..21460
                     /locus_tag="QBH27_002067"
     CDS             19796..21460
                     /locus_tag="QBH27_002067"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDQ7014377.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            21462..22406
                     /gene="mhpB"
                     /locus_tag="QBH27_002068"
     CDS             21462..22406
                     /gene="mhpB"
                     /locus_tag="QBH27_002068"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDQ7014378.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            22424..23290
                     /gene="mhpC"
                     /locus_tag="QBH27_002069"
     CDS             22424..23290
                     /gene="mhpC"
                     /locus_tag="QBH27_002069"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDQ7014379.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            23300..24109
                     /gene="mhpD"
                     /locus_tag="QBH27_002070"
     CDS             23300..24109
                     /gene="mhpD"
                     /locus_tag="QBH27_002070"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDQ7014380.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            24106..25056
                     /gene="mhpF"
                     /locus_tag="QBH27_002071"
     CDS             24106..25056
                     /gene="mhpF"
                     /locus_tag="QBH27_002071"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDQ7014381.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            25053..26066
                     /gene="dmpG"
                     /locus_tag="QBH27_002072"
     CDS             25053..26066
                     /gene="dmpG"
                     /locus_tag="QBH27_002072"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDQ7014382.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGGDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            26242..27453
                     /gene="mhpT"
                     /locus_tag="QBH27_002073"
     CDS             26242..27453
                     /gene="mhpT"
                     /locus_tag="QBH27_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDQ7014383.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESTVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNSMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            27555..28094
                     /locus_tag="QBH27_002074"
     CDS             27555..28094
                     /locus_tag="QBH27_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDQ7014384.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(28219..29052)
                     /gene="fghA"
                     /locus_tag="QBH27_002075"
     CDS             complement(28219..29052)
                     /gene="fghA"
                     /locus_tag="QBH27_002075"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDQ7014385.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSIAGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(29145..30254)
                     /gene="frmA"
                     /locus_tag="QBH27_002076"
     CDS             complement(29145..30254)
                     /gene="frmA"
                     /locus_tag="QBH27_002076"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDQ7014386.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(30289..30564)
                     /gene="frmR"
                     /locus_tag="QBH27_002077"
     CDS             complement(30289..30564)
                     /gene="frmR"
                     /locus_tag="QBH27_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDQ7014387.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
BASE COUNT         7078 a         7972 c         8415 g         7122 t
ORIGIN      
        1 atatcaaagt agtggtaaac ctatagcgta aattcagatt gttaaattaa ctctactact
       61 ttacacgtta gttatgagcg atttctgata gtgtctggtt taatcagagc tttattatct
      121 gcgacgttta tttttattta agagagccac ctgcaagttg ttattccacc agagagtgaa
      181 tcgcaaagca ggtcatcatt tcatctgtat atctgtatgc caaatggcat tccccctcct
      241 ttttcgccga tattttgttg tgatcgacga agtttcgctt aaaaaagcac cagcagtggt
      301 ttcgcagcta tatggtgtat aaaaaatgtt ctcatacaga tgttttgtga atgtgtgggt
      361 tgacattcat atgaaaaaaa tcataattcc atcatgtttg tgtgggggct tttctgtatc
      421 ttacgcatcg cactcaagcc tgacagaaaa tatgctgtaa ggctcatatc aaaaaccgcc
      481 attagctcat caggaagagc agacgacaac caggattgtt gtatggtacg gggttcgagg
      541 cctcgatggc ggtccaactt ttcaaaactc ttctgaatca gatcccctta ttatcatccc
      601 tgcaaagtgg aacattgcca ttgtttggct attcaggacg ggtcagacca atgaaaaagc
      661 ccacacaggg gagagtaggc tgaaatggga agctaaagac tcaagtaaac ttatcggaaa
      721 taaggaccac gcattacggg ggctatcatc gccgatggtt gggattcggc aaatcaggtt
      781 gattacgtag agagtaaatt atctgctcac cgctgcgtca ccccttcgta aatcaggcgt
      841 agcgcgaata caccaataat tgcaccaata acccgactgg caacgcgttg catacgccca
      901 taagcacgac gcacagcggg caaagaaaac gcctggctta gaaagactcg ccagataatt
      961 gatgtgagca caatccccgc ccaggccatt aatcgtgccc atgttggtgt ttcggcattt
     1021 aatgttactg agaaaatact gataaaaaat aaaacggttt gcgggttaga gagatcggta
     1081 attaatccac ggcggaaaaa gatataccag ggggcactaa tcggttgttg tagtgtgctc
     1141 atttgcggtg ttgactggcg gcgcatgctg caccacgcaa accataagag ataagcgccg
     1201 ccgacgattc tgataagcga aaaaatctcc tcacactgcg taattagcgt tgcaagacca
     1261 aacaaaccca acccggaata aaatgcatcg cccagcgcca cgcccagccc ggtcaacacc
     1321 cccgcgcgtc gaccggaagc caggctggtt tgtactacca caaagagatt ggctcccgga
     1381 ttaaaaaaag taatcacgaa caatcctacg gtcaggtaaa cggcgtgcaa aggatccata
     1441 gtgatttcat ccataaataa gtgaagtaac ttcatcatat ttctaccaaa aataatcggg
     1501 tgcgagagag atcacaaagt gtcttatttc cggttactgg cgtttatgcc ctgactgaac
     1561 taattattaa tcaacccaat aatgtgggtg ggtgatagtg tgataacaac tctggagccg
     1621 taatatgaaa ataatctcta aaatgttagt cggtgcgtta gcgtttgccg ttaccaatgt
     1681 ctatgccgca gaattaatga ccaaagcgga atttgaaaag gttgcatcgc aatatgaaaa
     1741 aataggtgat atttcaacca gtaatgaaat gtcgaccgca gatgcgaaag aagatttgat
     1801 caaaaaagcc gatgaaaaag gggctgatgt gttggtactg acctctggtc aaactgacaa
     1861 taagatccac ggcacggcaa atatttataa gaagaagtaa ttctgaatcc tatgtaaaca
     1921 tccccgatgc gttagtttat cggtgatcat catctatcga aatttatgcc ggataaagcg
     1981 ttcgcgctgc attcggcagt tcagcttttc agccgccgcc agaacgtcgt ccggctgata
     2041 cctaaataat tcgccgctgc tgttttatcg ccattaaatt tctccagtgc ctgttgtgct
     2101 gtcagcaagc ctggaatggg agtcttcgcc gactcgcgcg ccagttccgg cagtagcagc
     2161 tgcaaaaatt gcggcgttaa atccggcgtc gattccacac ttaaaaacag cgccagtcgt
     2221 tccatcatat tgcgcagttc acgaatattg cccggccagt cgtagtgcaa taatagaatc
     2281 tgacaggtct ctaatccctg acgtaatgcg gcagaaaaag ggacagagag tgccgccaga
     2341 gacattttca aaaagctttc cgccagcggc agaatatccg ccacccgctc gcgtagcggc
     2401 ggcagttgta gacgcaaaat actcagccga taaaacaggt cacggcgaaa ctgcccttgc
     2461 tgcatatctt cttccagatt gcagtgagtg gcgctaatga cccgcacatc caccggaaca
     2521 ggctgatgcc cgccgacgcg ggtgacctct ttttcttcca gcacccgcag cagccgagtc
     2581 tgcaacggca gcggcatttc gcctatctcg tcaagaaaca gcgtaccacc gtgggcgatt
     2641 tcaaacagcc cggcgcgtcc tccgcgtcgc gagccggtaa acgccccttc ctcatagcca
     2701 aacagctccg cttccagcag cgattcggca atcgccccgc agttaacggc aacaaacgga
     2761 tgtgactttt tgccctgtcg cgcatcgtgg cgggaaaaat attcccgatg aatcgcctgg
     2821 gccgccagct ctttgcccgt ccccgtttcc ccctcaatca acaccgccgc actggagcgg
     2881 gcatacagca aaatagtctg ccgcacctgt tccatctgtg gtgattgacc gagcatatcg
     2941 cccagcacgt aacgagtacg cagggcgttg cgggtggcat cgtgagtgtt atggcgtaac
     3001 gacatgcgcg tcatatccag cgcatcgctg aatgcctggc gcacggtggc ggcggaatag
     3061 ataaaaattc cggtcattcc ggcttcttct gccagatcgg taatcagccc tgcgccgacc
     3121 accgcttcgg tgccgttggc tttcaactca ttgatttgcc cgcgggcgtc ttcttcggtg
     3181 atgtagctac gttggtcgag gcgcaaatta aaggtctttt gaaacgccac cagcgctgga
     3241 atggtttcct gataggtgac aacaccgata gaagaggtga gttttccggc ttttgccagt
     3301 gcctgtaaca catcgtagcc gctcggttta atcaaaataa ctggcactga caggcgactt
     3361 ttaagatatg cgccgttaga gccagcagcg atgatggcgt cgcaccgttc gttggccagt
     3421 ttcttgcgga tgtaggtcac cgctttttca aagccaagct ggataggggt gatattcgcc
     3481 aggtgatcaa actcgaggct gatatcgcga aacagctcga acaggcgcgt tacagatacc
     3541 gtccagataa ccggtttgtc gtcattcagc cgtggtggat gtgccatagc gcaccgcaaa
     3601 gttaagaaac cgaatattgg gtttagtctt gtttcataat tgttgcaatg aaacgcggtg
     3661 aaacattgcc tgaaacgtta actgaaacgc atatttgcgg attacttcat gaatttatct
     3721 ttaactcatt gaaattaaat gtttaatttt attaaggcaa ttgtggcaca cccattgctt
     3781 tgtctttatc aacgcaaata acaagttgat aacaaaggat gggctatgtc tctacactct
     3841 ccaggtaaag cgtttcgcgc tgcactgact aaagaaaatc cattgcagat tgttggcact
     3901 atcaacgcta accatgcgct gctggcgcag cgtgccggat atcaggcgat ttatctctcc
     3961 ggcggtggcg tggcggcagg gtcgctgggg ctgcccgatc tcggtatttc tactcttgat
     4021 gatgtgctga ctgacattcg ccgtatcacc gacgtttgtt cgctaccgct gctggtggat
     4081 gcggatatcg gttttggttc ttctgctttt aacgtggcgc gcaccgtgaa atcgatgatt
     4141 aaagccggtg cggcaggatt gcatattgaa gatcaggttg gtgcgaaacg ctgcggtcat
     4201 cgcccgaaca aagcgatcgt ctcgaaagag gaaatggtgg atcggatccg tgcggcggtg
     4261 gatgcgaaaa ccgatcctga ttttgtgatc atggcgcgca ccgatgcgct ggcggtagag
     4321 gggctggatg cggcgatcga gcgtgcgcag gcctatgttg aagcgggtgc cgaaatgctg
     4381 ttcccggagg cgattaccga actcgccatg tatcgccagt ttgccgatgc ggtgcaggta
     4441 ccgatcctcg ccaacattac cgaatttggc gcaacaccgc tgtttaccac cgacgaatta
     4501 cgcagcgccc atgtcgcaat ggcgctctac ccgctttcag cgtttcgcgc catgaaccgc
     4561 gccgctgaac atgtctacaa catcctgcgt caggaaggca cacagaaaag cgtcatcgac
     4621 accatgcaga cccgcaacga gctgtacgaa agcatcaact actaccagta cgaagagaag
     4681 ctcgacgacc tgtttgcccg tggtcaggtg aaataaaaac gcccgttggt tgtattcgac
     4741 aaccgatgcc tgatgcgccg ctgacgcgac ttatcaggcc tacgaggtga actgaactgt
     4801 aggtcggata agacgcatag cgtcgcatcc gacaacaatc tcgaccctac aaatgataac
     4861 aatgacgagg acaatatgag cgacacaacg atcctgcaaa acagtaccca tgtcattaaa
     4921 ccgaaaaaat cggtggcact ttccggcgtt ccggcgggca atacggcgct ctgcaccgtg
     4981 ggtaaaagcg gcaacgacct gcattaccgt ggctacgata ttcttgatct ggcggaacat
     5041 tgtgaatttg aagaagtggc gcatctgctg atccacggca aactgccaac ccgtgacgaa
     5101 ctcgccgcct acaaaacgaa actgaaagcc ctgcgtggtt taccggctaa cgtgcgtacc
     5161 gtgctggaag ccttaccggc ggcgtcacac ccgatggatg ttatgcgcac cggcgtttcc
     5221 gcgctcggct gcacgctgcc agaaaaagag gggcataccg tctctggcgc acgggatatt
     5281 gccgacaaac tgctggcgtc acttagctcg attcttctct actggtatca ctacagccac
     5341 aacggcgaac gcattcagcc ggaaaccgat gacgactcta tcggcggtca cttcctgcat
     5401 ctgctgcacg gcgaaaagcc gtcgcaaagc tgggaaaagg cgatgcatat ttcgctggtg
     5461 ctgtacgccg aacacgagtt taacgcttcc acctttacca gccgggtgat tgcgggcact
     5521 ggctctgata tgtattccgc cattattggc gcgattggcg cactgcgcgg gccgaaacac
     5581 ggcggagcga atgaagtgtc gctggagatc cagcaacgct acgaaacgcc ggacgaagcc
     5641 gaagctgata tccgtaagcg ggtggaaaac aaagaagtgg tcattggttt tgggcatccg
     5701 gtttatacca tcgccgaccc gcgccaccag gtgatcaaac gcgtggcaaa gcagctctcg
     5761 caggaaggcg gctcgctgaa gatgtacaac atcgccgatc gcctggaaac agtgatgtgg
     5821 gagagcaaaa agatgttccc caatctcgac tggttctccg ctgtttccta caacatgatg
     5881 ggtgttccca ccgagatatt cacaccactg tttgttatcg cccgcgtcac tggctgggcg
     5941 gcgcacatta tcgaacaacg tcaggacaac aaaattatcc gtccttccgc caattatgtt
     6001 ggaccggaag accgccagtt tgtcgcgctg gataagcgcc agtaaacctc tacgaataac
     6061 aataaggaaa cgtacccaat gtcagctcaa atcaacaaca ttcgcccgga atttgatcgt
     6121 gaaatcgttg atatcgtcga ttacgtgatg aactacgaaa tcagctccag agtagcctac
     6181 gacaccgcac attactgcct gctcgacacg ctcggctgtg gtctggaagc tctcgaatat
     6241 ccggcctgta aaaaactgct ggggccaatt gtccccggca ccgtcgtacc caatggcgtg
     6301 cgcgtccccg gaactcagtt tcagctcgac cccgtccagg cggcatttaa cattggcgcg
     6361 atgatccgtt ggctcgattt caacgatacc tggctggcgg cggagtgggg gcatccttcc
     6421 gacaacctcg gcggcattct ggcaacggcg gactggcttt cgcgcaacgc ggtcgccagc
     6481 ggcaaagcgc cgttgaccat gaaacaggtg ctgaccgcaa tgatcaaagc ccatgaaatt
     6541 cagggctgca tcgcgctgga aaactccttt aaccgcgtcg gcctcgacca cgttctgtta
     6601 gtgaaagtgg cttccaccgc cgtggtcgcc gaaatgctcg gcctgacccg cgaggaaatt
     6661 ctcaacgccg tttcgctggc atgggtagac ggacagtcgc tgcgcaccta tcgccatgcg
     6721 ccgaacaccg gtacgcgtaa atcctgggcg gcgggcgatg ccacttcccg cgcggtacgt
     6781 ctggcactga tggcgaaaac cggcgaaatg ggttatccgt cagccctgac tgcgccggtg
     6841 tggggcttct acgacgtctc ctttaaaggc gaatcgttcc gcttccagcg cccgtatggt
     6901 tcttacgtga tggaaaatgt gctgttcaaa atctccttcc cggcggagtt ccactcccag
     6961 acggcagttg aagcagcgat gacgctctat gaacagatgc aggcagcagg caaaacggcg
     7021 gcggatatcg aaaaagtgac cattcgcacc cacgaagcct gtattcgcat catcgacaaa
     7081 aaagggccgc tcaataaccc ggctgaccgc gaccactgca ttcagtacat ggtggcgatc
     7141 ccgctgctgt tcgggcgctt aacggcggca gattacgagg acaacgttgc gcaagataaa
     7201 cgcatcgacg ctctgcgcga gaagatcaat tgctttgaag atccggtgtt taccgctgac
     7261 taccacgacc cggaaaaacg cgccatcgcc aatgccataa cccttgagtt caccgacggc
     7321 acgcgatttg aagaagtggt ggtggagtac ccaattggtc atgctcgccg ccgtcaggat
     7381 ggcattccga agctggtcga taaattcaaa atcaatctcg cgcgccagtt cccgactcgc
     7441 cagcagcagc gcattctgga ggtttctctc gacagaactc gcctggaaca gatgccggtc
     7501 aatgagtatc tcgacctgta cgtcatttaa gtaaacggcg gtaaggcgta agttcaacag
     7561 gagagcatta tgtcttttag cgaattttat cagcgttcga ttaacgaacc ggagcagttc
     7621 tgggccgagc aggcccggcg tattgactgg cagacgccct ttacgcaaac gctcgaccac
     7681 agcaacccgc cgtttgcccg ttggttttgt gaaggccgaa ccaacttgtg tcacaacgct
     7741 atcgaccgct ggctggagaa acagccagag gcgctggcat tgattgccgt ctcttcggaa
     7801 acagaggaag agcgtacctt taccttccgc caactgcatg acgaagtgaa tgcggtggcg
     7861 tcaatgctgc gctcgctggg cgtgcagcgt ggcgatcggg tgctggtgta tatgccgatg
     7921 attgccgaag cgcatattac cctgctggcc tgcgcgcgca ttggtgctat tcactcggtg
     7981 gtgtttgggg gttttgcctc gcacagcgtg gcggcgcgaa ttgatgacgc taaaccggtg
     8041 ctgattgtct cggctgatgc cggggcgcgc ggcggtaaaa tcattccgta taaaaaattg
     8101 ctcgacgatg cgataagtca ggcacagcat cagccgcgtc acgttttact ggtggatcgc
     8161 gggctggcga aaatggcgcg cgttagcggg cgggatgtcg atttcgcgtc gttgcgccat
     8221 cagcacatcg gcgcgcgggt gccggtggca tggctggaat ccaacgaaac ctcctgcatt
     8281 ctctacacct ccggcacgac cggcaaacct aaaggtgtgc agcgtgatgt cggcggatat
     8341 gcggtggcgc tggcgacctc gatggacacc atttttggcg gcaaagcggg cggcgtgttc
     8401 ttttgtgctt cggatatcgg ctgggtggta gggcattcgt atatcgttta cgcgccgctg
     8461 ctggcgggga tggcgactat cgtttacgaa ggattgccga cctggccgga ctgcggcgtg
     8521 tggtggaaaa ttgtcgagaa atatcaggtt agccgcatgt tctcagcgcc gaccgccatt
     8581 cgcgtgctga aaaaattccc taccgctgaa attcgcaaac acgatctttc gtcgctggaa
     8641 gtgctctatc tggctggaga accgctggac gagccgaccg ccagttgggt gagcaatacg
     8701 ctggatgtgc cggtcatcga caactactgg cagaccgaat ccggctggcc gattatggcg
     8761 attgctcgcg gtctggatga cagaccgacg cgtctgggaa gccccggcgt gccgatgtat
     8821 ggctataacg tgcagttgct caatgaagtc accggcgaac cgtgtggcgt caatgagaaa
     8881 gggatgctgg tagtggaggg gccattgccg ccaggctgta ttcagaccat ctggggcgac
     8941 gacgaccgct ttgtgaagac gtactggtcg ctgttttccc gtccggtgta cgccactttt
     9001 gactggggca tccgcgatgc tgacggttat cactttattc tcgggcgcac tgacgatgtg
     9061 attaacgttg ccggacatcg gctgggtacg cgtgagattg aagagagtat ctccagtcat
     9121 ccgggcgttg ccgaagtggc ggtggttggg gtgaaagatg cgctgaaagg gcaggtggcg
     9181 gtggcgtttg tcattccgaa agagagcgac agtctggaag accgtgaggt ggcgcactcg
     9241 caagagaagg cgattatggc gctggtggac agccagattg gcaactttgg ccgcccggcg
     9301 cacgtctggt ttgtctcgca attgccaaaa acgcgatccg gaaaaatgct gcgccgcacg
     9361 atccaggcga tttgcgaagg acgcgatcct ggggatctga cgaccattga tgatccggcg
     9421 tcgttggatc agatccgcca ggcgatggaa gagtaggtcg gataaggcgc tcgcgccgca
     9481 tccgacaccg tgcgcagatg cctgatgcga cgctgacgcg tcttatcatg cctacaggcc
     9541 tgaaccgtag gtcggataag gcgctcgcgc cgcatccgac accatgcgca gatgcctgat
     9601 gcgacgctga cgcgtcttat catgcctacc cactgttttt acacagataa gtttcccccg
     9661 cctttttgta ctcattcata taagaaatat atccccatac gaaaacgatt gctttttatc
     9721 ttcagatgaa tagaatgcgg cggatttttt gggtttcaaa cagcaaaaag ggggaatttc
     9781 gtgtcgcaag ataacaactt tagccagggg ccagtcccgc agtcggcgcg gaaaggggta
     9841 ttggcattga cgttcgtcat gctgggatta accttctttt ccgccagtat gtggaccggc
     9901 ggcactctcg gaaccggtct tagctatcat gatttcttcc tcgcagttct catcggtaat
     9961 cttctcctcg gtatttacac ttcatttctt ggttacattg gcgcaaaaac cggcctgacc
    10021 acccatcttc ttgctcgctt ctcgtttggt gttaaaggct catggttgcc ttcactgctg
    10081 ctgggcggca cccaggttgg ctggtttggc gttggcgtgg cgatgtttgc tattccggtg
    10141 ggcaaggcaa ccgggctgga tattaatttg ctgattgccg tctccggttt actgatgacc
    10201 gtcaccgtct tctttggcat ttcggcgctg acagttcttt cggtgattgc ggttccggct
    10261 atcgcctgtc ttggcggtta ttccgtgtgg ctggccgtta acggcatggg cggcctggac
    10321 gcattaaaag cggtcgttcc cgcacaaccg ttagatttca atgtcgcgct ggcgctggtt
    10381 gtggggtcat ttatcagtgc gggcacgctc accgctgact ttgtccggtt tggtcgtaat
    10441 gccaaactgg cggtgctggt ggcgatggtg gcctttttcc tcggcaactc gttgatgttt
    10501 attttcggtg cagcgggcgc ggcggcactg gggatggcgg atatctctga tgtgatgatt
    10561 gctcagggcc tgctgctgcc tgcgattgtg gtgctggggc tgaatatctg gaccaccaac
    10621 gataacgcgc tctatgcgtc gggtttaggt ttcgccaaca ttaccggtat gtcgagcaaa
    10681 accctttcgg tgattaacgg tattatcggt acggtctgcg cattatggct gtataacaat
    10741 tttgtcggct ggttgacctt cctttcggca gctattcctc cggtgggcgg cgtgatcatc
    10801 gctgattatc tgatgaaccg tcgccgctat gagcactttg cgaccacgcg tatgatgagt
    10861 gtcaattggg tggcgattct ggctgtcgcg ctggggattg ccgcaggcca ctggttaccg
    10921 ggaattgttc cggtcaacgc ggtattaggt ggcgcgctga gctatctgat ccttaacccg
    10981 attttgaatc gtaaaacgac agcagcaatg acgcatgtgg aggctaacag tgtcgaataa
    11041 tgctttacaa acaattatta acgcccggtt acccggcaaa gaggggctgt ggcagattca
    11101 ttttcacgac ggaaaaatca gcgccattga tgcgcaatcc ggcgtgatgc ccgtaactga
    11161 aaacagcctg gatgccgaac aaggtttagt tttaccgccg tttgtggagc cgcatattca
    11221 cctggacacc acgcaaaccg ccggacaacc gaactggaat cagtccggca cgctgtttga
    11281 aggcattgaa cgctgggccg agcgcaaagc gttattaact catgacgatg tgaaacaacg
    11341 cgcatggcaa acgctgaaat ggcagattgc caacggcatt cagcatgtgc gtacccatgt
    11401 cgatgtttcg gacgcaacgc tgactgcgct gaaagcaatg ctggaagtga aacaggaagt
    11461 tgcgccgtgg attgatctac aaatcgtcgc cttcccgcag gaagggattt tgtcgtatcc
    11521 caatggtgaa gcgttgctgg aagaggcgtt acgcttaggg gcagacgttg ttggggcgat
    11581 tccgcatttt gaatttaccc gtgaatatgg cgtggagtcg ctgcataaaa ccttcgccct
    11641 ggcgcaaaaa tacgaccgtc tcatcgatgt tcactgtgac gagattgatg atgagcaatc
    11701 acgctttgtc gaaaccgttg ctgccctggc gcaccgtgaa ggcatgggcg cgcgagtcac
    11761 cgccagccat actacggcaa tgcactccta taacggggcg tatacttcgc gcctgtttcg
    11821 tttgctgaaa atgtccggta ttaactttgt cgccaacccg ttggtcaata ttcatctgca
    11881 aggacgtttc gatacgtatc caaaacgtcg cggcatcacg cgcgttaaag agatgctgga
    11941 gtccggcatt aacgtctgct ttggtcacga tgatgtcttc gatccgtggt atccgctggg
    12001 aacggcgaat atgctgcaag tgctgcatat ggggctgcat gtttgccagc tgatgggcta
    12061 tgggcagatt aacgacggcc tgaatttaat cacccaccac agtgccagaa cgttgaattt
    12121 gcaggattac ggcattgccg ccggaaacag cgccaacctg attatcctgc cggctgaaaa
    12181 tgggtttgac gcgctgcgcc gtcaggttcc ggtgcgctat tcggtacgtg gcggcaaggt
    12241 gattgccagc acaaaaccgg cacaaaccac cgtatatctg gagcagccag aagccatcga
    12301 ttacaaacgg taatcggctg ggttaccgcg agcgtagttt atgccggatg cggcgtgaac
    12361 accttatccg gcctacgtag atcactgaaa ttgtaggcct gataagcgga gcgcatcagg
    12421 caattttgct tatttaaact gaagactcaa ctttaaaatc tttgaaataa tattgcttat
    12481 cccggtcgtt tatttcgcga ataacccgac aaggaacgcc tgcagccacg acgtttggtg
    12541 gaatgtcttt tgtgacgaca ctacccgcgc caataacaga attatccccg atggtgatgc
    12601 ctggattaat aaccacatga catccgatcc agacgttatt gccaatcgtt atcggaaaag
    12661 agtacatctc tccgtttttt ctcaattcat ggtgtaccgg gtgtcccgta acggaaagag
    12721 taacgttggg tgcaatcagt acgttatcac cgatcgttac cgtgtagtcg tcgacaatgg
    12781 ttaaattgaa atttgcataa aaattgcggc ctatatggat gttggaaccg taagagaaat
    12841 agacgggggg gtctacccag gcgttttccc ctaccgtggc aaacatttct ttaatcagac
    12901 tttccctttt ttcaaattct gatggatgcg agtgattaaa ctcatacatt aacgtttttc
    12961 cacgaagtct tttttcaggt aagccttcgc acatatcggt aaatagcttg cctgctttta
    13021 ttctttctgt catcgacatg ttcattgcga tcattccgcc ctgatatgtt ggtcggataa
    13081 ggcgctcgcg ccgcatccga cattgattgc ttaagcgact tcattcacct gacgacgcag
    13141 tagagaaagc gggccggggc cgctaagcgt gaacacggaa attaaggtga agcccagcgc
    13201 caccagaccc agcaccagat aagcgccctg gaaaccgatg ctttcataca tattgcccgc
    13261 cagtacagac ataaaaatca tcgccagttg cttaaagaag cagaaacaga ccagataaat
    13321 cgtcgctgaa aaacgcactt caaactggct ggtaatatat ttaaagcagc ccaccagcag
    13381 gaacggtact tcaaacatat gcagcgtttt cagaataacc acttccagcg ctgaggtggc
    13441 gaacgatgag ccaataatac gtacagacat aatagtgcca gccaccagca gggcattttt
    13501 cccaccgatg cgattaatga tcagtggcgc aaagaacata atcgaggcat taagtaattc
    13561 gcccattgtc gttacgtagc caaatacccg cgtaccctgt tcaccggtag caaaaaacga
    13621 agtaaagaaa ttagcaaact gttggtcaaa aacatcgtag gtgcaggaaa cgccaataac
    13681 atacagtgac aaaaaccaca gttttggctg tctgaacagt tccagcgcca gcttaaggct
    13741 aaatgccgaa tggttggcac ctaccgcatt ggcaaccgtg gctgaagagg gcgcatccgt
    13801 tttggcgaaa aagagtaaaa cggcgaggat gagtgcacag ccagaaccca gccagaaaac
    13861 aaactgatta ttgatggtga acatgatgcc gacaatcgag gcacacagcg cccagccaac
    13921 acagccaaac atccgcgcgc gaccaaattc gaaatgactg cgacggctga ctttctcgat
    13981 aaatgcctct actgctggcg caccggcgtt aaaacaaaag ccaagataaa taccaccaac
    14041 aatcgatcct actaaaatgt tgtattgtaa cagtggtccg aagataaaaa taaagaacgg
    14101 cgcaaacatc actaacatgc cggtaataat ccacagcagg tatttgcgca gcccgagttt
    14161 gtcagaaagc agaccaaaca gcggttggaa taatagcgag aacagagaaa tagcggcaaa
    14221 aataataccc gtatcacttt tgctgatatg gttgatgtca tgtagccaaa tcgggaaaaa
    14281 cgggaagtag gctcccatga taaaaaagta aaagaaaaag aataaaccga acatccaaaa
    14341 gtttgtgttt tttaaatagt acataatgga tttccttacg cgaaatacgg gcagacatgg
    14401 cctgcccggt tattattatt tttgacacca gaccaactgg taatggtagc gaccggcgct
    14461 cagctggaat tccgccgata ctgacgggct ccaggagtcg tcgccaccaa tccccatatg
    14521 gaaaccgtcg atattcagcc atgtgccttc ttccgcgtgc agcagatggc gatggctggt
    14581 ttccatcagt tgttgttggc tgtagcggct gatgttgaac tggaagtcgc cgcgccactg
    14641 gtgtgggcca taattcaatt cgcgcgtccc gcagcgcaga ccgttttcgc tcgggaagac
    14701 gtacggggta tacatgtctg acaatggcag atcccagcgg ttaaaacagg ctgcagtaag
    14761 gcggtcggga tagttttctt gcggccccag gccgagccag tttacccgct ctgcgacctg
    14821 cgccagctgg caggtcaggc caatccgcgc cggatgcggt gtatcgctcg ctacttcaac
    14881 acctacactg acgaccatct cgccgtgtcc atcaatccgg taggttttcc ggctgataaa
    14941 taaggttttc ccctgatgct gccacgcgtg ggcggtcgta atcagcaccg cgtcggcaag
    15001 tgtatctgcc gtgcactgca acaacgccgc ttcggcctgg taatggccag ccgccttcca
    15061 gcgttcgacc caggcgttag ggtcaatgcg ggtcgcttca cttacgccaa tgtcgttatc
    15121 cagcggcgca cgggtgaact gatcgcgcag cggggtcagc agttgttttt catcgccaat
    15181 ccacatctgt gaaagaaagc ctgactggcg gttaaattgc caacgcttat tacccagctc
    15241 gatgcaaaaa tccgtttcgc tggtggtcag ttgagggatg gcgtgggacg cggaggggag
    15301 tgtcacgctg aggttttccg ccagacgcca ttgctgccag gcgctgatgt gtccggcttc
    15361 tgaccatgcg gtcgcgtttg gttgcactac gcgtaccgtt agccagagtt gtccggcgct
    15421 ctccggctgc ggcagttcag gcagttcaat caactgctta ccttgcgggc caacatccag
    15481 aggcacttca ccgcttgcca gcggcttgcc atccagcgcc accatccagt gcaggaactc
    15541 gttatcgcta tgacggaaca ggtattcgct ggtcacttcg atggttcgcc cggataaacg
    15601 gaactggaaa tactgctgtt ggtgttttgc ttccgtcagc gccggatgcg gcgtgcggtc
    15661 ggcaaagacc agaccgttca tacagaactg gcgatcgttc ggcgtatcgc caaaatcacc
    15721 gccgtaagcc gaccacgggt tgccgttttc atcatattta atcagcgact gatccaccca
    15781 gtcccagacg aagccgccct gtaaacgggg gtactgacga aacgcctgcc agtatttagc
    15841 gaagccgcca agactgttac ccatcgcgtg ggcatattcg caaaggatca gcgggcgcat
    15901 ttctccaggc agcgaaagcc attttttgat ggaccatttc ggcaccgccg ggaagggctg
    15961 gtcttcatcc acgcgcgcgt acatcgggca aataatatcg gttgcggagg tgtcggctcc
    16021 gccgccttca tactgtaccg ggcgggaagg atcgacagat ttgatccagc gatagagtgc
    16081 gtcgtgatta gcgccgtggc ctgactcatt ccccagcgac cagatgatca cactcgggtg
    16141 attacgatcg cgctgcacca tccgcgttac gcgttcgctc atcgcgggta gccagcgcgg
    16201 atcatcggtc agacgattca ttggcaccat gccgtgggtt tcaatattgg cttcatccac
    16261 cacatacagg ccgtaacggt cgcacagcgt gtaccacagc ggatggttcg gataatgcga
    16321 acagcgcacg gcattaaagt tgttctgctt catcagcagg atatcctgca ccatcgtctg
    16381 ctcatccatg acctgaccat gcagaggatg atgctcgtga cggttaacgc cgcgaatcag
    16441 caacggcttg ccgttcagca gcagcagacc attttcaatc cgcacctcga ggaaaccgac
    16501 atcgcaggct tctgcttcaa tcagcgtgcc gtcggcggtg tgcagtttaa ccaccgcacg
    16561 atagagattc gggatttcgg cgctccacag ttccggattt tcaacgttca ggcgtagcgt
    16621 gacgcgatcg gcataaccgc cacgctcatc gataatttca ccgccgaaag gcgcggtgcc
    16681 gctggcgacc tgcgtttcac cctgccacaa agaaaccgtc acccgcagtt catcgcgcag
    16741 ctcgccgtac atctgaactt ctgcctccag taccgcgcgg ctgaaatcat cattaaagcg
    16801 agtggtaact tggaaatcgc tgatttgcgt ggtcggttta tgcagcaacg agacgtcacg
    16861 gaaaatgccg ctcatccgcc acatatcctg atcttccaga taactgccgt cactccagcg
    16921 cagcaccatc accgcgaggc ggttttctcc ggcgcgtaaa aatgcgctca ggtcaaattc
    16981 agacggcaaa cggctgtcct ggccgtaacc gacccagcgc ccgttgcacc acagatgaaa
    17041 cgccgagtta acgccatcaa aaataattcg cgtctggcct tcctgtagcc agctttcatc
    17101 aacattaaat gtgagcgagt aacaacctgt cggattctcc gcgggaacaa acggcggatt
    17161 gaccgtaatg ggataggtta cgttggtgta gatgggcgcg tcgtaaccgt gcatctgcca
    17221 gtttgagggg acgacgacag tatcggcgtc aggaagatcg cactccagcc agctttccgg
    17281 caccgcttct ggtgccggaa accaggcaaa gcgccattcg ccattcaggc tgcgcaactg
    17341 ttgggaaggg cgatcggtgc gggcctcttc gctatttcgc cagctggcga aagggggatg
    17401 tgccgcaagg cgattaagtt gtgtaacgcc agggttttcc cagtcacgac gttgtaatac
    17461 gacggccaga gaatccgtaa tcatagtcat agctgtatcc tgtgtgaaat tgttatccgc
    17521 tcacaatttc acacaacata cgagccggac acatagagtg taaagactgg ggtgcctaat
    17581 gagtgagctg actcacatta attgtgttgc gctcactgcc cgctttccag tcgggaaact
    17641 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    17701 ggcggaaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
    17761 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
    17821 tcctgtttga tggtggttaa cggcgggata taacacgagc tgtcttcggt atcgtcgtat
    17881 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
    17941 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcatttagc
    18001 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttctgttatc
    18061 ggctgaattt gattgcgtgt gagatattta tgccagcccg ccagacgcag acgcgccgag
    18121 acagaactta atgggcctgc taacagcgcg atttgctggt gacccaatgc gaccaggtgc
    18181 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggagtctgg
    18241 tcagagacat caagaaataa cgccggaaca ttagcgcagg cagcttccac agcaatggca
    18301 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
    18361 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
    18421 gcacccagtt gatcggcgcg agattttatc gccgcaacaa tttgcgacgg cgcgtgcagg
    18481 gccagactgg aagtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
    18541 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
    18601 gctgaaacgt ggcaggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
    18661 tactctgcga catcgtatag cgttactggt ttcacattca ccaccctgaa ttgactctct
    18721 tccgggcgct atcatgccat accacgaaag gttttgcgcc attcgatggt gtcagcgtaa
    18781 atgcacgccg cttcgccttc ccgctaccag aatagcctgc gattcaaccc cttcttcgat
    18841 ctgttttgcc acccgttgca gcgccggaag atgcttttcc gctgcctgtt cgatggtcat
    18901 tgcgctcgcc atatacacca gattcagaca gccaatcacc cgttgttcac tgcgcagcgg
    18961 tacggcgata gaggcgatct tctcctcctg atcccagccg cggtagttct gtccgtaccc
    19021 ctctttgcgc gcgcgcgcca gaatggcttc cagctttaac ggttctcgtg ccagttgata
    19081 gtcatcaccg gggcgggagg ctaacatctc gattaattcc ttgcgttctt gttccgggca
    19141 aaaggccagc caggtcaggc ccgaggcggt tttcagaagc ggtaaccgtc gcccgaccat
    19201 tgcccggtga aaagataagc ggctgaaacg atgggtggtt tcgcgtacca ccattgcatc
    19261 aacatccagc gtggacacat ctgtcggcca taccacttcg cgcaacagat cgcccagcag
    19321 cggggccgcc agtgcagaaa tccactgttc gtcacgaaat ccttcgctta attgccgcac
    19381 tttgatggtc agacgaaaac tgtcatcgga ggtactgcgc cggacatatc cctcttccag
    19441 taacgtctcc agcagtcgcc gcacagtggt gcgatgcagg ccgctgagtt ccgccagcag
    19501 cccgacgctg gcaccgccat caagtttatt taacatattt aataacatta gaccgcgggt
    19561 taagccgcgc acggttttgt attccgtctg ctcattgttc tgcatattaa ttgacattta
    19621 tatagttaaa acaacgttgt gcacctggtg cacattcggg catgttttga ttgtagccga
    19681 aaacaccctt cctatactga gcgcacaata aaaaatcatt tacatgtttt taacaaaata
    19741 agttgcgctg tactgtgcgc gcaacggcat tttgtccgag tcgtgaggta ctgaaatggc
    19801 aatacaacac cctgacatcc agcctgctgt taaccatagc gttcaggtgg cgatcgctgg
    19861 tgccggtccg gtcgggctga tgatggcgaa ctatctcggc cagatgggca ttgacgtgct
    19921 ggtggtggag aaactcgata agttgatcga ctacccgcgt gcgattggta ttgatgacga
    19981 ggcgctgcgc accatgcagt cggttggcct ggtcgataat gttctgccgc acactacgcc
    20041 gtggcacgcg atgcgttttc tcaccccaaa aggtcgctgt tttgctgata ttcagccaat
    20101 gaccgatgaa tttggctggc cgcgccgtaa cgcctttatt cagcctcagg tcgatgcggt
    20161 gatgctggaa ggattgtcgc gttttccgaa tgtgcgctgc ctgttttccc gcgagctgga
    20221 ggccttcagc cagcaagatg acgaagtgac cttgcacctg aaaacggaag aagggcagcg
    20281 ggaaacggtc aaagcccagt ggctggtggc ctgtgatggt ggggcaagtt ttgtccgtcg
    20341 caccctgaat gtgccgtttg aaggtaaaac tgcgccaaat cagtggattg tggtagatat
    20401 cgccaacgat ccgttaagta cgccgcatat ctatttgtgt tgtgatccgg tgcgcccgta
    20461 tgtttctgcc gcgctgcctc atgcggtacg tcgctttgaa tttatggtga tgccgggaga
    20521 aaccgaagaa cagctgcgtg agccgcaaaa tatgcgcaag ctgttaagca aagtgctgcc
    20581 taatccggac aatgttgaat tgattcgcca gcgtgtctac acccacaacg cgcgactggc
    20641 gcaacgtttt cgtattgatc gcgtactgct ggcgggcgat gccgcgcaca tcatgccggt
    20701 gtggcagggg cagggctata acagtggtat gcgcgacgcc tttaacctcg catggaaact
    20761 ggcgttggtt atccagggga aagcccgcga tgcgctgctc gatacctatc aacaagaacg
    20821 tcgcgatcac gccaaagcga tgattgacct gtccgtgacg gcgggcaacg tgctggctcc
    20881 gccgaaacgc tggcagggta cgttacgtga cggcgtttcc tggctgctga attatctgcc
    20941 gccagtaaaa cgctacttcc tcgaaatgcg cttcaagccg atgccgcaat attacggcgg
    21001 tgcgctggtg cgtgagggcg aagcgaagca ctctccggtc ggcaagatgt ttattcagcc
    21061 gaaagtcacg ctggaaaacg gcgacgtgac gctactcgat aacgcgatcg gcgcgaactt
    21121 cgcggtaatt ggctggggat gcaatccact gtgggggatg agcgacgagc aaatccagca
    21181 gtggcgcgcg ttgggcacac gcttcattca ggtggtgccg gaagtgcaaa ttcataccgc
    21241 acaggataac cacgacggcg tactacgcgt gggggatacg caaggtcgcc tgcgtagctg
    21301 gttcgcgcaa cacaatgctt cgctggtggt gatgcgcccg gatcgctttg ttgccgccac
    21361 cgccattccg caaaccctgg gcaagaccct gaataaactg gcgtcggtga tgacgctgac
    21421 ccgccctgat gccgacgttt ctgtcgaaaa ggtagcctga tatgcacgct tatcttcact
    21481 gtctttccca ctcgccgctg gtggggtatg tcgacccggc acaagaggta ctcgatgagg
    21541 tcaatggcgt gattgccagc gcccgcgagc gtattgcggc tttctcccct gaactggtgg
    21601 tgctgtttgc gccagatcac tacaacggct ttttctatga cgtgatgcca ccgttctgtt
    21661 taggcgttgg agcgacggca attggtgatt tcggcagtgc ggcaggagag ctgcccgtgc
    21721 ctgtggagct ggcggaggcc tgtgcgcatg ccgtcatgaa gagcgggatc gatcttgccg
    21781 tttcttactg tatgcaggtg gaccacgggt tcgcccagcc gctggagttc ctgctcggtg
    21841 ggctggataa ggtgccagta ctgccggtgt tcatcaacgg tgtcgccacg ccgctgcccg
    21901 gtttccagcg tacccgcatg ttgggggaag ccattggacg tttcaccagt actctcaata
    21961 aacgcgtgct gttcctgggt tccggtgggc tttcccatca gccgccggtg cccgaactgg
    22021 cgaaagccga tgcccatatg cgcgaccgtc tgttggggag cgggaaagat ttacccgcca
    22081 gtgagcgcga attgcgtcag caacgggtga ttagcgccgc tgagaagttt gttgaggatc
    22141 agagaacgct gcatccgctc aacccgattt gggataacca gttcatgact ttgctggagc
    22201 agggacgcat acaggaactg gatgccgtca gtaacgaaga gctttccgcc attgccggaa
    22261 agtcgacaca tgaaatcaaa acctgggtcg ccgcttttgc cgctatttct gcgtttggca
    22321 actggcgtag cgaagggcgt tattaccgcc caatcccgga gtggattgcc ggatttggct
    22381 cgttaagcgc cagaacagag aactgaatat gcaggagaag atgatgagtt atcagccaca
    22441 aaccgaagcc gccaccagcc gttttctgaa tgtagaagaa gcgggtaaaa cgctgcgcat
    22501 ccattttaat gactgcggac aaggcgacga aaccgttgtc ctgctgcatg gttccggccc
    22561 gggtgctact ggctgggcga acttcagccg caatatcgat ccgctggtag aggcgggcta
    22621 tcgggtgatc ctgctggatt gtccgggttg gggtaagagc gattcgatcg ttaatagtgg
    22681 ttcgcgatcg gatcttaatg cgcgaatcct gaaaagcgtg gtggatcaac tggatatcgc
    22741 caaaatccac ctgctgggca actcgatggg tggccacagt tctgtggcgt tcactcttaa
    22801 ctggccggag cgcgtcggca aactggtgct gatgggcggc ggtacgggcg gcatgagttt
    22861 gtttacgccg atgccaaccg aaggtattaa gcgactgaat cagctttatc gtcagccgac
    22921 tatcgaaaac ctgaagctga tgatggatat cttcgttttt gataccagcg atttgaccga
    22981 cgccctgttt gaagcgcgcc tgaataatat gctgtcgcgc cgcgatcacc tggaaaactt
    23041 cgttaagagc ctggaagcca atccgaaaca gttcccggat tttggcccac gtctggcgga
    23101 aatcaaagcg caaaccctga ttgtctgggg gcgcaacgac cgctttgtgc cgatggatgc
    23161 gggtctgcgt ctgctgtccg gcattgccgg ttctgaactg catatcttcc gcgactgtgg
    23221 tcactgggcg cagtgggaac atgccgacgc tttcaatcaa ctggtgctga atttcctcgc
    23281 acgcccttaa ggaacggtca tgacgaagca tactcttgag caactggcgg cggatttacg
    23341 ccgcgccgca gagcagggcg aagcgattgc gccgctgcgc gatctgattg gtatcgataa
    23401 cgctgaagcg gcctacgcca ttcagcacat caatgtgcaa catgacgttg cgcaggggcg
    23461 tcgcgtggtg gggcgcaaag tggggctgac gcatccgaaa gtgcagcaac aactgggcgt
    23521 tgatcaaccg gatttcggga cattatttgc cgacatgtgt tatggcgata atgaaaccat
    23581 tcctttttcc cgtgttctgc aaccccgcat tgaagcggag attgcactgg tgttgaaccg
    23641 cgatctaccc gcaaccgata tcaccttcga cgaattgtat aacgccattg aatgggtact
    23701 tccggcgctg gaggtggtgg ggagccgcat tcgcgactgg tcgattcagt ttgtcgatac
    23761 cgtggcagat aacgcttcct gcggggtgta tgtcatcggc ggtccggcgc aacgcccggc
    23821 ggggttagac ctgaaaaact gcgccatgaa gatgacgcgt aataacgaag aggtttccag
    23881 cgggcgcggc agcgaatgcc tgggacatcc gcttaatgcc gccgtctggc tggcacgcaa
    23941 aatggccagt ctgggtgaac cgctgcgcgc cggagatatc attcttaccg gggcattagg
    24001 tccgatggtg gcggtgaatg cgggcgatcg ttttgaagcc catatcgaag gcataggttc
    24061 agttgctgcg acattttcaa gcgcagcccc aaaaggaagt ctgtcatgag taagcgtaaa
    24121 gtcgccatta tcggttcagg caacattggc accgatctga tgattaaaat tttgcgtcac
    24181 ggtcagcatc tggagatggc ggtgatggtc ggcattgatc ctcagtccga cggtctggcg
    24241 cgtgccagac gtatgggcgt cgccaccacc catgaagggg tgatcgggct gatgaacatg
    24301 cctgaatttg ctgatatcga cattgtattt gatgcgacca gcgcgggcgc gcatgtgaaa
    24361 aacgatgccg ctttgcgcga agcgaaaccg gatattcgct taattgacct gacgcctgct
    24421 gccatcggcc cttactgcgt gccggtggtt aacctcgagg agaacgtcgc tcaactgaac
    24481 gtcaacatgg tcacctgcgg cggccaggcc accattccaa tggtagcggc agtttcacgc
    24541 gtggcgcgtg ttcattacgc cgaaattatc gcttctattg ccagtaaatc tgccggaccc
    24601 ggcacgcgtg ccaacatcga tgaatttacg gaaaccactt cgcgcgccat tgaagtggtg
    24661 ggtggcgcgg caaaagggaa ggcgattatt gtgcttaacc cagcagagcc accgttgatg
    24721 atgcgtgaca cggtgtatgt attgagcgac gaagcttcac aagatgatat cgaagcctca
    24781 atcaatgaaa tggctgaggc ggtgcaggct tacgtaccgg gttatcgcct gaaacagcgc
    24841 gtgcagtttg aagtcatccc gcaggataaa ccggtcaatt taccgggcgt ggggcagttc
    24901 tccgggctga aaacggcggt ctggctggaa gtcgaaggcg cagcgcatta tctgcctgcc
    24961 tatgcgggca acctcgacat tatgacttcc agtgcgctgg cgacagcgga aaaaatggcc
    25021 cagtcactgg cgcgcaaggc aggagaagcg gcatgaacgg taaaaaactt tatatttcgg
    25081 acgtcacgtt gcgtgacggt atgcacgcca ttcgtcatca gtattcgctg gaaaatgtcc
    25141 gccagattgc caaagcactg gatgatgccc gcgtggattc gattgaagtg gcccacggcg
    25201 acggtttgca gggttccagc tttaactatg gtttcggcgc acatagcgac cttgaatgga
    25261 ttgaagcggc ggcggatgtg gtgaagcacg ccaaaatcgc gacgttgttg ctgccaggaa
    25321 tcggcactat tcacgatctg aaaaatgcct ggcaggctgg cgcgcgggtg gttcgtgtgg
    25381 caacgcactg taccgaggct gatgtttccg cccagcatat tcagtatgcc cgcgagctcg
    25441 gaatggacac cgttggtttt ctgatgatga gccatatgac cacgccggag aatctcgcaa
    25501 agcaggcaaa gctgatggaa ggctacggtg cgacctgtat ttatgtggtg gattctggcg
    25561 gtgcgatgaa catgagcgat atccgtgacc gtttccgcgc cctgaaagca gtgctgaaac
    25621 cagaaacgca aaccggcatg cacgctcacc ataacctgag tcttggcgtg gcgaattcta
    25681 tcgcggcggt ggaagagggc tgcgaccgaa tcgacgccag cctcgcggga atgggcgcgg
    25741 gcgcaggtaa cgcgccgctg gaagtattta ttgccgccgc ggataaactg ggttggcagc
    25801 atgggggcga tctctatgcg ttaatggatg ccgctgacga cctggtgcgt ccgttgcagg
    25861 atcgaccggt acgagtcgat cgcgaaacgc tggcgctggg atacgctggt gtttactcca
    25921 gcttcctgcg tcactgtgaa acggcggcgg cgcgttatgg cttaagcgcg gtggatattc
    25981 tcgttgagct gggcaaacgc cggatggttg gcggccagga ggatatgatc gtcgacgtgg
    26041 cgctggatct gcgcaacaac aaataatatt gactgccgag aacgtgcatt ttgtatgccc
    26101 atcccagcac gactgccgga tgcggcgtga acgccttatc cggcctacat ttcgcccgta
    26161 ggcagtcatt aaatagttct gattacgcgg ctggcaatgt atcagtcgcg attcacctgc
    26221 actcgcaatg aggttcatct tatgtcgact cgtacccctt catcatcttc atcccgcctg
    26281 atgctgacca tcgggctttg ttttttggtc gctctgatgg aagggctgga tcttcaggcg
    26341 gctggcattg cggcgggtgg catcgcccag gctttcgcac tcgataaaat gcaaatgggc
    26401 tggatattta gcgccggaat actcggtttg ctacccggcg cgctggttgg cgggatgctg
    26461 gcagaccgtt atgggcgtaa gcgtattttg attggctcag ttgcgctgtt tggtttgttc
    26521 tcgctggcaa cggcgattgc atgggatttc ccctcactgg tctttgcgcg gctgatgacc
    26581 ggtgtcgggc tgggggcggc gttgccgaat cttatcgccc tgacgtctga agccgcggga
    26641 ccacgttttc gtgggacggc agtgagcctg atgtattgcg gtgttcccat tggcgcggcg
    26701 ctggcggcga cgctgggttt cgcgggggca aacttagcat ggcaaacggt gttttgggta
    26761 ggtggtgtgg tgccgttgat tctggtgccg ctattaatgc gctggctgcc ggagtcgacg
    26821 gttttcgctg gcgaaaaaca ggctgcgcca ccactgcgtg cgttatttgc gccagaaacg
    26881 gcaaccgcga cgctgctgct gtggttgtgt tattttttca ctctgctggt ggtctacatg
    26941 ttgatcaact ggctgccgct gcttttggtg gagcaaggat tccagccatc gcaggcggca
    27001 ggggtgatgt ttgcactgca aatgggggcg gcaagcggga cgttaatgtt gggcgcattg
    27061 atggataaac tgcgtccagt aaccatgtcg ctactgattt atagcggcat gttagcttcg
    27121 ctgctggcgc tgggaacggt gtcgtcattt aacagtatgt tgctggcggg atttgtcgcg
    27181 gggttgtttg cgacaggtgg gcaaagcgtt ttgtatgctc tggcaccgtt gttttacagt
    27241 tcgcagatcc gcgcaacagg tgtgggaaca gccgtggcgg tagggcgtct gggggctatg
    27301 agcggtccgt tactggccgg gaaaatgctg gcattaggca ctggcacggt cggcgtaatg
    27361 gccgcttctg caccgggtat tcttgttgct gggttggcgg tgtttatttt gatgagtcgg
    27421 agatcaagaa tgcagccgtg tgccgatgcc tgacgcgcct tattaagcag gggcgaaagg
    27481 gacttgcccc caatacacct acggcgttta caggtatact cgctaaaaat tattcagcgg
    27541 gtttggaaac aaagatggca aaacttacct tacaagagca gttgctcaaa gcgggactag
    27601 tcaccagcaa aaaagcggcg aaggtcgaga gaacggcgaa aaaatcgcgt gttcaggcgc
    27661 gtgaagcacg ggcggcggta gaagaaaata aaaaggcaca gcttgagcgt gataaacagc
    27721 ttagcgagca acaaaaacaa gcggcgttgg cgaaagaata taaagcccag gtgaagcagc
    27781 tcattgaaat gaaccgaatt accattgcca atggcgatat tggttttaac ttcactgacg
    27841 gcaatctgat taagaagatt tttgtcgata agctcacgca aacgcagttg attaatggtc
    27901 gtctggcgat tgcccgcttg ttggtcgata acaatagcga aggtgaatac gccattattc
    27961 ccgccagcgt tgccgataaa attgctcagc gtgatgccag cagtattgtg ttgcacagcg
    28021 cgctcagcgc cgaagagcag gatgaagacg atccgtatgc cgacttcaaa gtgcctgatg
    28081 atttgatgtg gtaaacattg ataaatcgtg tgcagatgcc tgatgcgacg cttttcgcgt
    28141 cttatcaggc ctacggttta cgagcgaaat gtgggccgga taaggtttac gccgcatccg
    28201 gcatgcgtgc gcggatgctc aacgcatatt cagtttattg gcgtggtagg caatatgctc
    28261 gccaataaaa ctggagacaa aataatagct gtgatcataa ccctcgtgat aacggattaa
    28321 cgtcttgata ttcatctcct ggcagatctt ttcaagattt ggagtccgca gttgttccgc
    28381 gtaaaaatca tcactcaacc cctgatcaac catgatttcc gcaacgcgtt gaccttgtga
    28441 aataagactc accggatcgt aatccaacca ggcatcttta ttttcaccaa gatatgcggc
    28501 aaaggcttgc tgtccccacg gtacttgcga tggggagaca atgggcgaaa acgccgagac
    28561 gctgacatat tcatctgggt tacgtaacgc cagcaccagc gcgcccagcc cgcccataga
    28621 atgacctgcg attgactttc tggccgttgc cggaaaatga tgcatcacta aattcggcag
    28681 ctcgttgcgg atatagtcat acattttgta atgttcattc cacggcgctt gcgtcgcgtt
    28741 caggtaaaac ccggcacctt gcccgagatc gtaacggtca gcatctgcga catgactgcc
    28801 tcgcggactg gtgtccggcg caacaacaat aatgttgtgc tcagccgcgt aacgctgcat
    28861 ccccgattta gtaatgaaat tttgctcgtt gcaggtcagg cctgaaagcc agtacaacac
    28921 cggcaatttt tcattctcgg cttttggggg gagatagacg ccgacattca tttcacattt
    28981 cagtgattgg gaataatgcc gatacacatt ttgccagccg ccaaagctgg catgtttttc
    29041 aatgagttcc atcagtcatt atctcacggt taatcccccg gacggctgcc cggggagagt
    29101 attaagaacc ccatctgaac tggacggggt aaaccagcgg gaaatcagta acgaattacg
    29161 gttcgaatgg atttaccttc atgcatcagg tcgaaggcgt cattaatttc atccaggctc
    29221 atggtatgcg tgacaaacgg ttccagatcg atatcacctt tcatcgcatc ttcaaccata
    29281 cccggtaact gggaacgacc tttcacgccg ccaaacgcgg aacctttcca tacgcgaccg
    29341 gtgaccaact ggaatggacg ggtggagatt tcctgaccgg agcctgctac cccgatgatc
    29401 accgactgac cccagccgcg gtgcgcgctt tccagcgctg cacgcatcac gttgacgtta
    29461 ccgatgcatt caaaggtatg gtcgataccc catttgttga tatccagcag gacatcttta
    29521 atcggtttgt cgtagtcatt cgggttaatg cagtcggtgg caccgaagcg acgcgccagt
    29581 tcgaatttct tcgggttggt atcgatagcg ataatccgtc ccgcttttgc ctgacgcgcg
    29641 ccctgaacca ctgccagacc aatcgcgcca agaccaaaca cggcaacaga atcacctggc
    29701 tggactttag ctgtgttgtg taccgcgcca ataccggtgg tcacgccaca gcccagcagg
    29761 cagacgtgtt catggtttgc ttctggatta attttggcca gagacacttc cgctaccacg
    29821 gtgtattcac tgaatgttga gcatcccatg tagtgataaa gcggctgccc gttgtaagaa
    29881 aaacgggtgg tgccgtctgg catcaagcct ttgccctgcg tttcgcgaac cgcgacacac
    29941 aggttggttt tgccagaacg acaaaactca cactcgccgc actctgcggt gtaaagtggg
    30001 atcacatggt cgccaggttt gacgctggtt acgccttcac cgacttcaac tacaaccccg
    30061 gccccttcgt gaccgagaac cactgggaag acaccttccg ggtcatcgcc ggagagggta
    30121 aatgcgtcgg tatggcaaac accggtatgg gtgactttaa tcagcacttc accttttttc
    30181 ggtggtgcaa cgtcaatttc aacgatttcc agcggtttac cgggagcaaa tgcaacggca
    30241 gcacgtgatt tcatctctcg ctcttcctca atatggtaat agattcagct atttaagata
    30301 ggcacgaacc agttcaatag tgtcgtcaac ggattggctg acttcacggc tgtagcagtc
    30361 atttcggtca aacgtttccc ggatatggct ttcaagcact tctgccatca gtccgttagc
    30421 cgcgccccga acggcagcga tctgttggag tatggcacgg cattcggcat caccctccag
    30481 cgaccgttcc agagcatcaa tctgcccccg aatacgacga actcgagtaa ggaccttttt
    30541 cttctcttcc ggagtactgg gcatttcgca cctcatcatc tgcacgc
//
LOCUS       DAOFSV010000043        30552 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.043, whole genome shotgun sequence.
ACCESSION   DAOFSV010000043 DAOFSV010000000
VERSION     DAOFSV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.043"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..470)
                     /gene="yeiL"
                     /locus_tag="QBH27_002078"
     CDS             complement(<1..470)
                     /gene="yeiL"
                     /locus_tag="QBH27_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDQ7014388.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAA"
     gene            600..1541
                     /gene="rihB"
                     /locus_tag="QBH27_002079"
     CDS             600..1541
                     /gene="rihB"
                     /locus_tag="QBH27_002079"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDQ7014389.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            complement(1809..2897)
                     /locus_tag="QBH27_002080"
     CDS             complement(1809..2897)
                     /locus_tag="QBH27_002080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ7014390.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(2901..3758)
                     /gene="nfo"
                     /locus_tag="QBH27_002081"
     CDS             complement(2901..3758)
                     /gene="nfo"
                     /locus_tag="QBH27_002081"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDQ7014391.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(3832..4881)
                     /locus_tag="QBH27_002082"
     CDS             complement(3832..4881)
                     /locus_tag="QBH27_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDQ7014392.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGTNSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            4980..5861
                     /locus_tag="QBH27_002083"
     CDS             4980..5861
                     /locus_tag="QBH27_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7014393.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            6066..7535
                     /gene="lysP"
                     /locus_tag="QBH27_002084"
     CDS             6066..7535
                     /gene="lysP"
                     /locus_tag="QBH27_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDQ7014394.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            7827..9818
                     /gene="cirA"
                     /locus_tag="QBH27_002085"
     CDS             7827..9818
                     /gene="cirA"
                     /locus_tag="QBH27_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDQ7014395.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(9850..10686)
                     /gene="yeiG"
                     /locus_tag="QBH27_002086"
     CDS             complement(9850..10686)
                     /gene="yeiG"
                     /locus_tag="QBH27_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDQ7014396.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYQMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            10944..11612
                     /gene="folE"
                     /locus_tag="QBH27_002087"
     CDS             10944..11612
                     /gene="folE"
                     /locus_tag="QBH27_002087"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDQ7014397.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11629..12786
                     /locus_tag="QBH27_002088"
     CDS             11629..12786
                     /locus_tag="QBH27_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDQ7014398.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAINNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLSLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            12928..13968
                     /gene="galS"
                     /locus_tag="QBH27_002089"
     CDS             12928..13968
                     /gene="galS"
                     /locus_tag="QBH27_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDQ7014399.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14248..15246
                     /gene="mglB"
                     /locus_tag="QBH27_002090"
     CDS             14248..15246
                     /gene="mglB"
                     /locus_tag="QBH27_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDQ7014400.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15307..16827
                     /gene="mglA"
                     /locus_tag="QBH27_002091"
     CDS             15307..16827
                     /gene="mglA"
                     /locus_tag="QBH27_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDQ7014401.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            16843..17853
                     /gene="mglC"
                     /locus_tag="QBH27_002092"
     CDS             16843..17853
                     /gene="mglC"
                     /locus_tag="QBH27_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDQ7014402.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18096..19331)
                     /gene="preA"
                     /locus_tag="QBH27_002093"
     CDS             complement(18096..19331)
                     /gene="preA"
                     /locus_tag="QBH27_002093"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDQ7014403.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGS
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(19325..20563)
                     /gene="preT"
                     /locus_tag="QBH27_002094"
     CDS             complement(19325..20563)
                     /gene="preT"
                     /locus_tag="QBH27_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDQ7014404.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIQQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            complement(20757..20996)
                     /locus_tag="QBH27_002095"
     CDS             complement(20757..20996)
                     /locus_tag="QBH27_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDQ7014405.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(20999..21718)
                     /gene="sanA"
                     /locus_tag="QBH27_002096"
     CDS             complement(20999..21718)
                     /gene="sanA"
                     /locus_tag="QBH27_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDQ7014406.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(21868..22752)
                     /gene="cdd"
                     /locus_tag="QBH27_002097"
     CDS             complement(21868..22752)
                     /gene="cdd"
                     /locus_tag="QBH27_002097"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDQ7014407.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(22882..23577)
                     /locus_tag="QBH27_002098"
     CDS             complement(22882..23577)
                     /locus_tag="QBH27_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDQ7014408.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(23574..23972)
                     /locus_tag="QBH27_002099"
     CDS             complement(23574..23972)
                     /locus_tag="QBH27_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDQ7014409.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            24211..25161
                     /gene="dusC"
                     /locus_tag="QBH27_002100"
     CDS             24211..25161
                     /gene="dusC"
                     /locus_tag="QBH27_002100"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDQ7014410.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(25549..25632)
                     /gene="yohP"
                     /locus_tag="QBH27_002101"
     CDS             complement(25549..25632)
                     /gene="yohP"
                     /locus_tag="QBH27_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDQ7014411.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            25856..27292
                     /gene="mdtQ"
                     /locus_tag="QBH27_002102"
     CDS             25856..27292
                     /gene="mdtQ"
                     /locus_tag="QBH27_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDQ7014412.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            27345..28106
                     /locus_tag="QBH27_002103"
     CDS             27345..28106
                     /locus_tag="QBH27_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7014413.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(28236..28814)
                     /locus_tag="QBH27_002104"
     CDS             complement(28236..28814)
                     /locus_tag="QBH27_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ7014414.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            28984..29571
                     /locus_tag="QBH27_002105"
     CDS             28984..29571
                     /locus_tag="QBH27_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDQ7014415.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            29745..>30552
                     /gene="pbpG"
                     /locus_tag="QBH27_002106"
     CDS             29745..>30552
                     /gene="pbpG"
                     /locus_tag="QBH27_002106"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDQ7014416.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFAD"
BASE COUNT         7581 a         7580 c         7924 g         7467 t
ORIGIN      
        1 gctgccaggc gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga
       61 taatttttat gacttaaggt gacgcagagt tttcgtaaaa atagcgtgtc gtttaataac
      121 agcgggcggt aatgcttcat agggagcgca aggcaccaac actcttcaat agcctgcact
      181 gcacgcggtt catggtcttt gtcgattaac tcaatctcgc caataaaaca gggcgcggca
      241 aagaaatcga tcagcgacac gcgaccatta gcaagcgtga cgtaaagcct ggcgcggcct
      301 cgcgtcaggt aaaacagcca ggacggctgt tgcccttcct gcacaatgta gtcacgcgct
      361 aaaaaatgaa acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca
      421 gattcactca tcaattgctg cttaagatca ttattgtgga tttctttcac gatgtttatc
      481 cgctatgaga tttctcatat ttagcatacg tacgctgttg ttaagattca atcatcggcc
      541 aggtcgaatt ttctttgctg tagaaacaat aacgcaactg gaaacagagg aaataaacaa
      601 tggaaaagag aaaaattatt ctggattgtg atccgggtca tgatgatgct attgctataa
      661 tgatggcggc gaaacatcct gcgattgatt tattaggtat caccattgta gcgggtaatc
      721 aaaccctcga taaaacatta attaatggcc tgaatgtttg ccagaaactg gagattaatg
      781 ttccggttta tgcggggatg ccgcagccca ttatgcgtca acaaattgtt gccgataata
      841 ttcacggtga aaccggactg gatggcccgg tattcgagcc gctgacccgc caggcagaaa
      901 acactcatgc ggtgaaatat atcatcgata cccttatggc aagcgatggc gatatcactc
      961 tggtgccggt tggtccgttg tcaaacatcg cggtggcaat gcgtatgcaa cccgcgatcc
     1021 tgccgaaaat ccgtgaaatt gttctgatgg gcggcgctta tggtactggc aacttcaccc
     1081 catctgctga gttcaatatc tttgctgacc cggaagccgc acgcgtggtg ttcacctccg
     1141 gcgttccatt agtgatgatg ggcctcgatc ttaccaacca gaccgtttgc accccggacg
     1201 tgattgctcg gatggaaagg gctggcggcc ctgcgggaga gctgttcagc gacatcatga
     1261 acttcactct caaaacgcag ttcgaaaact acggcctggc tggcggcccg gtgcacgacg
     1321 ccacctgcat cggttatttg attaaccctg atggcattaa aacccaggag atgtacgtcg
     1381 aagtggacgt taacagtggc ccttgctatg ggcgtaccgt ctgcgacgag ttgggcgttc
     1441 ttggcaagcc cgccaatacc aaagtcggca tcactattga tacagactgg ttctggggat
     1501 tagtcgaaga gtgcgtacgc ggctacatca aaacccatta agtgttgata acaagccggg
     1561 tcagtatcgc aagatgcagg cccggctatt tatgatgggc agagagccgt cgcccaaaat
     1621 gacatgaaga gataataact atggatgtca tgagaagtgt tctgggaatg gtggtattgc
     1681 tggctattgc gtttttgctg tcagtaacct gatgagtgct actattgcag gattctttat
     1741 tggtttagcg tagtgcgcaa tttatgagcc ggataagatg cggtcaagcg tcgaatccgg
     1801 caacattgtt acaccattgg cactaatgaa agcgcgttat cggcagacag ggtagggttg
     1861 tttaagctgc cgctggcacg cgaaattgcc gcgcaggcta tcgcaaaacg ggcgctgtcg
     1921 cggaaactgt atccttcaag aaagctatat accaggcccg ccataaaacc atcgtcagca
     1981 ccaaaactgt ctactgtcgt atgcgctggt gcggtcagca aaaattgttc tccatcctta
     2041 tcgctgcaaa atacagattc atctttcaga taaacaaaaa tgcgtttaac accttgttga
     2101 tgcagcgaat ttacagctct gatgcgatcg ttgtcgtctt taatcggttg cccccacaaa
     2161 atttccagtt cattttgtgt tggtttcaga gtgtggatcc ggctgtacca gttttttact
     2221 ttgttcgctt taaattcgga aacggtatcg acaaacatcg gaatttcatc agcaatggta
     2281 aagacccact ccagggcttc aggtgtgaga ttacaatcag ccagcaccac accagcatgg
     2341 cgaatcaagt cccgtgaagt attgagcagt tgaggcgtta gctgttgcag aatgtgtgtg
     2401 tcattgattg ccaggatagt ttcttcctgc ttattagcga tagcgagata agttgcagtg
     2461 ctgtgtccat gcagacgaat gcagtttgaa acgttcacgc ccgcgcggcg cgtttcttcc
     2521 agtaatgtct cgccgtaaaa atcgttgcca atagctgaaa ttaaatgcac atcccgacca
     2581 agcagagcca ggttatgcgc gatattacgt ccgacgccgc ctgcggaaca gtgaacagaa
     2641 cctggatgag aggctgcttg cgggtaacgg atatccgcca ttccgcgaat atccatattg
     2701 attgcaccca caaccacaca gtaatcctgc tcggtaagga tatatccttt gcctttaatc
     2761 aaacccttgc gcatcaaatc cataatatga gcggcaacac gtgagcggct gatttgcaga
     2821 atatcggcga tttcattctg ttgaatcagc gggttacgcc gtaaaatttt aagaatctgt
     2881 ttttccctgt cgttcatcga tcaggccacc gctttttcag tttgttgcgc tttcagccag
     2941 gcgatctctt ctgcccagat atccgggttg atggtttcaa ggatcagcgg aatgccgtcg
     3001 aaacggtcgt cctgcatgat ccagcggaac gcatcatgac cgatattgcc ttcgccgagg
     3061 ctatgatgac ggtcaacgcg gctgccaaag gtgcttttcg catcgttgag gtgcatcccg
     3121 cgcagatact taaagcctac aatacgggca aaatcagcga acgttttctc gcattcggca
     3181 ggagtacgca aatcataccc ggcagcgaaa gcatggcagg tatcaatgca gacgccgacg
     3241 cgggatttat cttccacgcc gtcgataatc gccgcgagat gttcgaattt aaaccctaag
     3301 ttactgccct gaccggcggt gttttcaatc accgcggtga caccttgagt tttatccagc
     3361 gcaatgttga tggattcggc aatacgcgca aggcaatcct cttctgaaat ctgcatcaga
     3421 tggctgccag ggtggaagtt gagcaaagaa agccccagct gttcgcaacg ctgcatttca
     3481 tctataaagg catcgcgcga tttttccaga gcttcagcaa ccggatgtcc gaggttaatc
     3541 agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct
     3601 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
     3661 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
     3721 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
     3781 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gatgtagtgg tttatgcgat
     3841 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
     3901 cagtgccatc agcagaggtt tcgccccggc ttttttcagc gcgctgacgt gagtggtcag
     3961 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
     4021 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca acgacgatga acaagatggc
     4081 aaaccacgga atagtgattt tgcttttctc gccgctgttc gtcccagaca gctgtttaac
     4141 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
     4201 aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
     4261 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
     4321 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
     4381 aacagccacg gttactttac tggcttctgc tttcactacc ggctcagtcg ccagtaccgc
     4441 ggcagcacca cagatactgc taccggcacc gatcaaccag ctggtgtgct tatccagacc
     4501 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcaacacgtc
     4561 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
     4621 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
     4681 tttccagatg tgcgggtaaa tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
     4741 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
     4801 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
     4861 ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact
     4921 ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg taagcgacta
     4981 tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca
     5041 cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgaccgacc
     5101 tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg
     5161 aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg
     5221 aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta
     5281 actacattct acctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg
     5341 aacttagcgt tgggaatagc caggacgtga tgcaagcggt gctggatttc cgcgttgata
     5401 ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag
     5461 acgagctggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg gtcaccttag
     5521 aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggagattg
     5581 tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact
     5641 ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacggg
     5701 tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc
     5761 tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc
     5821 gctttctgga ctattgcgat cccgcgaatg taccgcgtta aggtgctgta caagaacatg
     5881 ctggtgctgt gtcgatttcg tgacgcagcg ccctcagcat gcattcgcca gaaaagagat
     5941 tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct
     6001 gccggaagga ttgccaatcg tctgctacaa tcgcgcctca tttttaagat ggatagcatt
     6061 tttgtatggg ttccgaaact aaaactacag aagcgccggg cttacgccgt gaattgaagg
     6121 cgcgtcacct gacgatgatt gccattggcg gttccatcgg tacaggtctt tttgttgcct
     6181 ctggcgcaac gatttctcag gcaggtccgg gcggggcatt gctctcgtat atgctgattg
     6241 gcctgatggt ttacttcctg atgaccagtc tcggtgaact ggctgcatat atgccggttt
     6301 ccggttcgtt tgccacgtac ggtcagaact atgttgaaga aggctttggc ttcgcgctgg
     6361 gctggaacta ctggtacaac tgggcggtga ctattgccgt tgacctggtt gcagcacagc
     6421 tggtcatgag ctggtggttc ccggatacac cgggctggat ctggagtgcg ttgttcctcg
     6481 gcgttatctt cctgttgaac tacatctctg ttcgcggctt tggtgaagcg gaatactggt
     6541 tctcactgat caaagtcacg acagttattg tctttatcat cgttggcgtg ctgatgatta
     6601 tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc ggcgaagcgc
     6661 cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat gattgtcggc ttctctttcc
     6721 agggaaccga gctgatcggt attgctgcag gcgagtccga agatccggcg aaaaacattc
     6781 cacgcgcggt acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc gcgatcctga
     6841 ttatcagcct gattattccg tacaccgatc cgagcctgct gcgtaacgat gttaaagaca
     6901 tcagcgtcag tccgttcacc ctggtgttcc agcacgcggg tctgctctct gcggcggcgg
     6961 tgatgaacgc agttattctg acggcggtgc tgtcagcggg taactccggt atgtatgcgt
     7021 ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt ttcgctaaac
     7081 tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt gccggtttgt
     7141 gcttcctgac ctccatgttt ggcaaccaga cggtatacct gtggctgctg aacacctccg
     7201 ggatgacggg ttttatcgcc tggctgggga ttgccattag ccactatcgt ttccgtcgcg
     7261 ggtacgtatt gcagggacac gacattaacg atctgccgta ccgttcaggt ttcttcccac
     7321 tggggccgat cttcgcattc attctgtgtc tgattatcac tttgggccag aactacgaag
     7381 cgttcctgaa agataccatt gactggggcg gcgtagcggc aacgtatatt ggtatcccgc
     7441 tgttcctgat tatttggttc ggctataagc tgattaaagg aactcacttc gtacgctaca
     7501 gcgaaatgaa gttcccgcag aacgataaga aataagtttc ctcccttcct tgctaagccc
     7561 tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg atataaaatt
     7621 taacatttgg attgataatt gttatcgttt gcattatcgt tacgccgcaa tcaaaaaagg
     7681 ctgacaaatc agaggctgtt ccggctttct gggatgatca cctgcataca aataagtcca
     7741 ccgcgatgct gccgtacgca aggggacgta aagaagatgt gagcgatacc cattttattt
     7801 tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc
     7861 tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa
     7921 cgatggttgt cactgcatct tccgttgaac aaaaccttaa agatgctccc gccagtatca
     7981 gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca
     8041 aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc gtaagtattc
     8101 gtggtctgga cagcagctac accctgattc ttgtcgacgg taaacgcgtt aactcccgca
     8161 atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat tccatcgaac
     8221 gtattgaagt ggtccgtggc ccgatgtcgt cgctgtacgg ttccgatgcg ctcggcggtg
     8281 tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggcaccgtt accgtcgata
     8341 ccaccgttca ggaacatcgc gatcgcggtg atacctataa cggtcagttc tttaccagcg
     8401 gaccattaat tgacggcgtg ctgggaatga aagcttacgg cagcctggca aaacgtgaaa
     8461 aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt attgaaggat
     8521 tctccagccg cgacggcaat gtcgaatttg cctggacacc gaatcaaaat cacgatttta
     8581 ctgccggata cggtttcgac cgtcaggatc gtgattccga ctcgctggac aaaaaccgcc
     8641 tggaacgcca gaactactcc gtcagccata atgggcgttg ggattacggc accagcgaac
     8701 tgaaatacta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc ccgataactt
     8761 ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt aatcagtttc
     8821 tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg
     8881 gaaccagctc caaaacgtct gccagccagt acgcgctgtt tgtggaagat gaatggcgga
     8941 tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa acctacggtg
     9001 aacactggag tccgcgtgcc tacctggttt ataacgccac cgacaccgta acggtgaaag
     9061 ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct gactggacga
     9121 gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa ccagaaacca
     9181 gcgaaagttg ggaactgggg ctttactaca tgggcgaaga aggctggctg gaaggggttg
     9241 aatccagcgt taccgttttc cgtaacgatg tgaaagatcg tatcagcatt agccgtacgt
     9301 ctgacgtcaa cgctgcaccg ggctaccaaa actttgtcgg ttttgagacg ggcgctaacg
     9361 gacggcgcat accggtattt agctactaca acgttaacaa agcacgtatt cagggcgtgg
     9421 aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcgatcaac tacacctaca
     9481 acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg ccgttccata
     9541 ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca ttctatgttt
     9601 ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt
     9661 ataccatctg gaataccggc gctgcctggc aggtgactaa agacgtcaaa ctgcgtgcag
     9721 gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat aacgaagacg
     9781 gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc cggcgaaccg
     9841 ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct ctataaaaga
     9901 ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca tcggccacgc
     9961 tttctggcgc gcggcttctg ccagtactgc aggttgcagt tggtcagcaa gaaactgatc
    10021 attatcaccc tgatcaataa gcgtcgggat tgcatcctgc gcgttactgg catacatcaa
    10081 cgcgcaactg tcccattcca gccatgcatt tttgtcctca cctaaataag tgctaaacgc
    10141 tttgattccc cacgggacgc tgcacggatt cacaattggc gcaaaggcgg aaacgctggt
    10201 gtatttaccc ggatttttca gcgccataat cagcgcgccg tgaccaccca tggaatgacc
    10261 gctaatagcg cagcggtcgc tgacattaaa ttgcgactga atcagtgccg gtaattcatc
    10321 gcgcagataa tcatacatct ggtaatgcgt cgcccacggt ggttgcgtgg cgttaagata
    10381 aaaccccgcg ccctggccta aatcgtagcc atcgtcgttg gcaacctgtt cgccgcgcgg
    10441 gctggtgtct ggcattacca gtacaatccc cagttccgcc gccacgcgct gggcacccgc
    10501 cttggtggtg aagttctcgt cattgcaggt taatccggaa agccagtaca gcactggtgg
    10561 cggggtgtga tcacgaggtg gtgggagaaa gatactgaac gtcatcgggc agtttaaggt
    10621 actggagtcg tgtcgccagc gttgctgcca gccttcaaaa cagcggtgct cttcgagcat
    10681 ttccatgcgt ggctccttgt tgtgttgttt gcaatttctt catcataccg tttattcaat
    10741 gtgctgtgag taactttcac ttccgtattt gcataacgat gttttaacat ctgctgatga
    10801 aaggcagcgg caattacaat aattatcgct gtgaatactg gattatgtgc gccgcctcac
    10861 gcacaataat caggctgtaa atcagcttaa taactttgcc cccacgcagg gcggaggcgt
    10921 cacacctgca ggagaaatca taaatgccat cactcagtaa agaagcggcc ctggttcatg
    10981 aagcgttagt tgcgcgagga ctggaaacac cgctgcgccc gcccgtgcat gaaatggata
    11041 acgaaacgcg caaaagcctt attgctggtc atatgaccga aatcatgcag ctgctgaatc
    11101 tcgacctggc tgatgacagt ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg
    11161 atgaaatttt ctccggtctg gattacgcca atttcccgaa aatcaccctc attgaaaaca
    11221 aaatgaaggt cgatgaaatg gtcaccgtgc gcgatatcac cctgaccagc acctgtgaac
    11281 accattttgt taccatagat ggcaaagcga cggtggctta tatcccgaaa gattcggtga
    11341 tcggtctgtc aaaaattaac cgcatcgtgc agttctttgc ccagcgtccg caggtgcagg
    11401 aacgtctgac gcagcaaatt ctcattgcgc tacaaacgct gctgggcacc aataacgtgg
    11461 ctgtctctat cgacgcggtg cattactgcg tgaaagcgcg tggcatccgc gacgcaacca
    11521 gtgccacaac aacgacctct cttggtggat tgttcaaatc cagtcagaat acgcgccacg
    11581 agtttctgcg cgctgtgcgt catcacaact gattaaaagg caggagccat ggagcgcaac
    11641 gtcacgctcg attttgttcg cggcgtcgcc attctgggga tcctgctatt aaacatcagc
    11701 gcctttgggc taccaaaggc ggcttatctc aatcccgcct ggtacggcgc tattacgccg
    11761 caggatgcat ggacctgggc atttctcgat ctcatcggcc aggtgaaatt cctcacgctt
    11821 tttgcgctgc tgtttggtgc gggcctgcaa atgttgctgc cccgtggcag acgctggatc
    11881 cagtcgcggt taacgctgtt agtcttgctg ggctttattc acggtttatt gttctgggac
    11941 ggcgatattc tgctggctta cggactggtg ggcttaatct gctggcggct ggtgcgcgat
    12001 gcgccatcgg taaaaagcct gtttaataca ggcgtcatgc tttatctggt ggggcttggc
    12061 gttttgctgt tattggggct gatttccgac agccagacca gccgcgcctg gacgccggat
    12121 gcatcggcta ttttgtatga aaaatactgg aagcttcacg gtggtgttga agcgatcaat
    12181 aatcgtgccg atggtgttgg caacagttta ctggcactgg gcgcacagta tggctggcaa
    12241 ctggcaggga tgatgctcat tggtgcggcg ctgatgcgca gcggctggct gaaagggcag
    12301 ttcagcttac gtcactatcg tcgtactggt tttgtgctgg tggcgattgg ggtgatcatt
    12361 aaccttcctg ccatcgccct gcaatggcag ctggactggg cgtatcgctg gtgtgccttc
    12421 ttacttcaaa tgccgcggga actgagtgcg ccgtttcagg cgattggcta tgcgtcgctg
    12481 ttttatggct tctggccgca attaagccgc tttaagctgg tgctggcaat cgcctgtgtc
    12541 ggacggatgg cactgaccaa ctatctattg caaacgctga tttgtactac gcttttttac
    12601 cacctcagtt tgtttatgca gtttgaccgt ctggagctgc tggcgtttgt tattccggta
    12661 tggctggcga atatcttctt ctctgttatc tggctgcgtt acttccgcca ggggccggtg
    12721 gaatggctct ggcgtcagtt aactttgcgt gctgccggac cgacaatatc taaaacatca
    12781 agataacgat ctggatcaca ttcgttaaca aaacggctgt aaccgtttcc attgctgtga
    12841 ctcgattcac gaagtcctgt attcagtgct gacaaaatag ccgccagcaa gcagtcattt
    12901 actgcaatct cataacaggt agtgaatatg atcaccattc gtgatgtagc gcgtcaggct
    12961 ggcgtctctg tggcaacggt ttcccgggtg ctcaataaca gcacgctggt cagtgccgac
    13021 acgcgtgaag cagtaatgaa agctgtgagt gagctggatt atcggccaaa cgccaatgcc
    13081 caggcgctgg caactcaggt tagcgacacc attggcgtgg tggtgatgga cgtttctgat
    13141 gcttttttcg gcgcgctggt aaaagcggtg gatctggtcg cccagcagca tcagaaatac
    13201 gtgctaatcg gcaatagcta tcatgaagcg gaaaaagagc gtcacgccat tgaggtgtta
    13261 attcgccagc gttgtaatgc gttgattgtt cactcaaaag cattgagtga cgatgaactg
    13321 gcacaattta tggataacat tcctggtatg gtgttaatca accgcgttgt gccggggtac
    13381 gcccatcgtt gcgtttgcct ggataatctc agcggtgccc gaatggcgac gcgcatgttg
    13441 ctgaataacg gtcatcaacg tattggttat ctttcttcca gccacggcat tgaagatgac
    13501 gccatgcgta aagcaggctg gatgagtgcg ttgaaagagc aggatattat tccgccggaa
    13561 agctggattg gcactggtac gccggacatg ccgggcggtg aggcggcgat ggttgaactg
    13621 ctggggcgca atctacaact aaccgctgta tttgcttata acgacaatat ggctgctggc
    13681 gcactgacag cattaaaaga taatggcatt gcgattccgc tacatctctc aatcatcggt
    13741 ttcgatgata ttcccatcgc ccgttacacc gacccgcaat taacgaccgt gcgttatccc
    13801 attgcttcaa tggctaaatt agctaccgaa ctggccttgc agggggcagc agacaatatt
    13861 gatcctcgtg ccagccactg ttttatgccg acgttagtgc gtcgccattc tgtcgcaaca
    13921 cgccagaatg cggcggcgat cactaactca acaaatcagg cgatgtaacc gctttcaatc
    13981 tgtgagtgat ttcacagtat cttaacaatg tgatggctat gattgcaccg ttttaacgtt
    14041 gtaacccata tgtaacagtg aataatcact ttcaccgagg taatagcgtc ataacaacaa
    14101 ttaaagccgt tttctggagc gttaccgggc atggaagaac gaattttaaa aagtgagctt
    14161 cggcgttcag taacacttca ttaactctac tgccccgccg agcatttatc tcaagcacta
    14221 ccctgcataa gaaaaaccgg agataccatg aataagaagg tgttaaccct gtctgctgtg
    14281 atggccagca tgttattcgg tgccgctgca cacgctgctg atactcgcat tggtgtaaca
    14341 atctataagt acgacgataa ctttatgtct gtagtgcgca aggctattga gcaagatgcg
    14401 aaagccgcgc cagatgttca gctgctgatg aatgattctc agaatgacca gtccaagcag
    14461 aacgatcaga tcgacgtatt gctggcgaaa ggggtgaagg cactggcaat caacctggtt
    14521 gacccggcag ctgcgggtac ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt
    14581 ttcttcaaca aagaaccgtc tcgtaaggcg ctggatagct acgacaaagc ctactacgtt
    14641 ggcactgact ccaaagagtc cggcattatt cagggcgatt tgattgctaa acactgggcg
    14701 gcgaatcagg gttgggatct gaacaaagac ggtcagattc aattcgtact gctgaaaggt
    14761 gagccggggc atccggatgc agaagcacgt accacttacg tgattaaaga attgaacgac
    14821 aaaggcatta aaactgaaca gttacagtta gataccgcta tgtgggatac cgctcaggcg
    14881 aaagataaga tggacgcctg gctgtctggc ccgaacgcca acaaaatcga ggtggttatc
    14941 gccaacaacg acgcgatggc aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc
    15001 agcattccgg tgtttggcgt cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt
    15061 gcactggcgg gcaccgtact gaacgatgct aacaaccagg cgaaagcgac ctttgatctg
    15121 gcgaaaaacc tggccgatgg caaaggtgcg gctgatggca ccaactggaa aatcgacaac
    15181 aaagtggtcc gcgtacctta tgttggcgta gataaagaca acctggctga attcagcaag
    15241 aaataagact gattgggcgc agtcgtttac tgcgccctta ttgtatgacc aacaaggtaa
    15301 aattatatgg tcagctcaac gactccgtca tcaggtgaat acttgttgga aatgagcggt
    15361 atcaacaagt cttttcctgg tgttaaggca cttgataacg ttaacttaaa agtccggcca
    15421 cattctatcc atgcattaat gggggaaaac ggtgcaggaa aatcgacatt attaaaatgc
    15481 ctgtttggta tttatcaaaa agactccggc accattttat tccagggtaa agagatcgat
    15541 ttccattccg ccaaagaagc actggaaaat ggtatttcga tggtacatca ggagttaaac
    15601 ctggtattac aacgttcggt gatggacaac atgtggctgg ggcgatatcc caccaaaggc
    15661 atgtttgtcg atcaggacaa aatgtaccgc gaaaccaaag cgatttttga tgaactggat
    15721 attgatatcg atccgcgtgc gcgcgtcggt acattatctg tttcgcaaat gcagatgatc
    15781 gaaatcgcca aagcgttttc ctataacgcg aaaattgtga ttatggatga accgacttct
    15841 tcgttaaccg aaaaagaggt caatcatctg ttcactatta ttcgtaaatt aaaagagcgc
    15901 ggctgcggta ttgtttatat ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa
    15961 gttaccgtat tgcgcgacgg tcagtggatc gctaccgaac cgctggcagg actgacgatg
    16021 gacaaaatca tcgccatgat ggttgggcgt tctcttaacc agcgtttccc tgacaaagaa
    16081 aacaagccgg gcgaagtgat tctggaagtg cgtaacctga cgtcgctgcg ccagccgtcg
    16141 attcgcgatg tctcgtttga tctgcataaa ggggagatcc tcggtattgc cggtctggtg
    16201 ggagcgaaac gtaccgatat tgttgagacg ttatttggta ttcgcgagaa atcggctggc
    16261 accattacgt tgcacggcaa aaagatcaat aaccataatg ccaacgaagc cataaaccac
    16321 ggatttgcac tggtaactga agagcgccgc tcaacgggaa tttatgccta tctggatatt
    16381 ggctttaact cgttaatttc caatatccgc aactataaaa ataaagttgg tttactggat
    16441 aactcgcgga tgaaaagcga tacccagtgg gtgattgatt cgatgcgggt aaaaacaccg
    16501 gggcatcgga cgcaaattgg ttcgctctcc ggtggtaatc agcaaaaagt gattattggt
    16561 cgctggctgt taacgcaacc agaaatatta atgctcgatg aaccgacgcg cggtattgat
    16621 gttggggcta agtttgagat ttatcagtta attgccgaac tggcgaagaa aggcaagggg
    16681 attattatta tctcctccga aatgccagag ttgttaggga taacagaccg tattctggtc
    16741 atgagcaatg gtctcgtttc cggaattgtc gatacaaaaa caacaacgca aagcgaaatt
    16801 ctgcgtcttg cgtctttgca cctttaagat taggggcttc ccatgagtgc gttaaataag
    16861 aaaagttttc ttacttacct gaaagagggc ggtatttacg tcgttctttt agttttgctg
    16921 gcgattatta ttttccagga cccaacattt ttaagtctgt tgaacttaag taatattctc
    16981 acccagtcat cggtgcgtat tattatcgcg cttggtgtgg cagggttaat tgtcacccag
    17041 gggactgacc tttccgcagg tcgccaggtg gggctggcgg cagtagtagc agcgacgtta
    17101 ttgcagtcca tggataacgc caacaaagtg ttcccggaaa tggcgacgat gccgattgcg
    17161 ctggttattc tgattgtctg tgccattggt gcggtgatcg gtttgatcaa cggcttgatt
    17221 atcgcctatc tcaacgtgac gccgttcatt accacgctcg gtacgatgat tatcgtctac
    17281 ggcattaact cgctctatta cgactttgtc ggggcgtcgc caatttccgg ttttgacagt
    17341 ggtttctcca cttttgccca aggctttatt gcgctgggga gtttccgtct ctcttacatc
    17401 accttctatg cgttgattgc ggtggtgttt gtctgggtgt tatggaacaa aacccgcttc
    17461 ggcaagaaca tttttgccat tggcggtaac ccggaagcgg cgaaagtatc tggtgtcaac
    17521 gtcggcctga acctgctgat gatctacgcg ttgtctggtg tgttctatgc cttcggcggg
    17581 atgttagaag ccggacgtat cggctctgcc accaacaacc tcggcttcat gtatgagctg
    17641 gatgctatcg cagcgtgcgt ggtaggcggc gtatcgttca gcggcggtgt agggacggtg
    17701 attggcgtgg tgaccggggt gattattttt accgtcatca actatggcct gacgtacatc
    17761 ggcgtaaacc cgtactggca gtacatcatc aaaggggcaa ttattatctt tgccgtggcg
    17821 ctggattcac tgaaatacgc gcgtaagaaa tgatgtgaat gtggtccgca ttcgttcata
    17881 aacgcacata acatcatgat ctgtgtggtt ttgtaggccg gataaggcgt ttacgccgca
    17941 tccggcgaca ggagccaaat gccggatgca acgctggcgc gttttatccg gcctacaatg
    18001 agcacaacat actgatttat attgttttgt atgccgggta aggcattcac gccgcatctg
    18061 gcgacaggaa ctaaatgccg gatgcgtcgc tggttttaca acgttaccgg gtgttctttc
    18121 tcgcctttct taaacttcac ttccccaaga tcaatacaac ctaccgggca gacgtgacca
    18181 caaagcagac aacccacgca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    18241 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    18301 catttatcaa gattgatatg gggatagaca atataactgc ggtctaaatc ttccgccggg
    18361 acaatattgt gattcgccag acctaccatc tcctgcaacg aatcaaaccc ttgatcggcg
    18421 agataatgcg acaacccgct tgccatatct tccactatcc gatacccgta ctgcataatg
    18481 ccggtggtca cctgcaacgt cgctgaaccg agcaataaaa actccgccgc atcttcccag
    18541 gtttcaatgc cgccgatacc gctgatcggg aaatcgcgca gttctggatg ggtgcgcatt
    18601 tgctgaataa agcgtagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    18661 gatttaccgt taacgatcgg cataccaatt ttctgattaa gatcgatatt ggtgatggat
    18721 ttaacggtgt taatcgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    18781 tcgcacatat caccaatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccacgt
    18841 tttactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    18901 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagcttct
    18961 tgcaccagac gcgccagctc ttcccattgc tgctcatttt cgcccatgat tgaagcgatc
    19021 aatactttgt ccgggtaatc ttctttcagc cgacgcaggg cggccagatt ctcttccaac
    19081 ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
    19141 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa aaccgatcgt tttaaacacc
    19201 acgccgcccc aacctgtgtc gtaggctttg gcgcacatct catagcagtt gcctaccggc
    19261 gaagaagaaa ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct
    19321 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgccccggcg gcttctttcc
    19381 cggttttcac tgcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga
    19441 cttgcgggtc gcgggtctgg taatgttgcg tttcgatggt gttacgttgc ggttccagtg
    19501 cggaaaaagc agcaagttcg gcatgttgac ctacagcgag aataattttg tccgccgcta
    19561 tcgttagttc acccggtaaa cgaacatgct taaaggtgac tttattgcct tccacggcta
    19621 ctggtgtaaa tccatcgatg atcgaaacgc ccagctctct ggcgctggta aactcttttt
    19681 cgcttgccgg aaactcatct aactcttcac gcgctacgca ggttaccgcc tgacagccga
    19741 gaactttcag cgtgctggct acgtccatcg cgacatcacc gccgccgata attaatgcgc
    19801 tttgttgaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaggaag tcaacggcaa
    19861 tctcaacgtc gctatgctca aacagcgata acccggaacc gctcgataac cctacggtga
    19921 ccagtaccgc gcggttttcc gctttcagct gctccagggt gagtgtttta ccgatttcgt
    19981 tattgcactt aatggtcacg cccatttttt ctatacgggc gatttctgca tccagcaccg
    20041 attgcggtaa ccggaattgc ggaatgccgt tacgcaacca gccaccgggt tgtgcttctt
    20101 tctcatagat cgtgacgtca taaccctggt ttgtcagcgt cacactggcc tgcaatccgg
    20161 caggacctgc gccaataatg gcgactttgc cgagcgtttt ggtaccgggc tgataaattt
    20221 ccatcccagt ttgttgttca aaatcagtaa caaaacgctg taagcgaccg atatcaatgg
    20281 gagcatcaac accggcacgt gtacaaccgc tttggcataa tttctccgtc gggcacactc
    20341 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
    20401 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
    20461 tacagggagc gtcatgacat aataaacagc gagaggcttc tttaatcgcc agtaaaggcg
    20521 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
    20581 tatgagtaat taaggcgaac gctaaataaa tattgcgtgc tgatattaag gctatgcgcg
    20641 tggggaaatt taaactgtga aaggattcac aaatagctgc ggttcagaat gttatatccg
    20701 cagcttattg agtgaagtaa taattattct caataattaa cagaaaagag aaaatattaa
    20761 cgatagaaaa taagataaac aatcatgccg atagataaaa ttccgaagcc gatatatgcc
    20821 accatataag cgaagaagag tccgggattt ttcgctcgcc agacatacac cggttccggt
    20881 gcatttttaa cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa
    20941 caaaatatcg ggataaggaa aaaaacggca acgacaaaaa actgctgtac atccataact
    21001 actttccttg tttcttttgt aattcaagta actgttcggg tgtgacagcg ggatacccct
    21061 gcgcatcttc cggcacttga tgcattgccg gaatagggac cagcggccct aaaaaacgcg
    21121 gttcacgttt aaaaatataa aggtcagcca gcgcaccgaa acgggcggca aattcgcgaa
    21181 tacgtactga cagcatatct ttcggtgacg gtacggcata acactgagcc tgaatcccca
    21241 tatgcagcgc aataaataat gcccgctcac agtggaaacg ttgggtgata ataatgaaat
    21301 cattagtatc gaaaactttg cgtgtacgta cgatggaatc cagcgtacga aagcctgcgt
    21361 aatcgagaac aatatctgat gggtcgacac cggctgcgat taaatctttg cgcatggtca
    21421 tcggctcatt ataactttgc aatgcgttat cgccgctcag taatagataa tttaccttac
    21481 cgctgttata ggcattaatc gctccttgaa tgcggtagcg ataatactga ttaattacgc
    21541 cagtccgata atattttgct gtgccgagca ccacaccgac ctggcggtag gggagatcct
    21601 gcaattcgtc gtagatataa ggcgcggttt tccagctcat ccagcgatcg aggccgagca
    21661 cagtcaacag cagcaagccg atcaggacta acaggctgag gaacacgcgc tttaacatgc
    21721 aggagactcg atctaaggaa aatttccatc aggctacttt acccgctggt tttgcgcaag
    21781 aaacggcaaa ctgtttgccg ggattttcga caccaggaaa gggctggcaa gccggacaag
    21841 gcgttcacgc cgcatccggc accaggctta agcgagaagc acgcggtcga tattgtgaca
    21901 gccgagagct ttcagcgttg cggaggtggc atcccactga atcaacggcg catcggcttt
    21961 ttctgccaga accgcgcgct ggatatccgg gtaatcataa cccttgagat tcaacagaat
    22021 taacgccccc tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc
    22081 gctgaaaata cgaccgtctt tacattccag cgcgacaccg cttggcgact tactgtaagg
    22141 catgtgcgaa cggtttgccg ccgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc
    22201 atagccgtga tcctgctcgt ccatcagcag cgttttaatc tccagatctt tcggcccaaa
    22261 ggcatccggt agatagtcac gcagcgcgtg tgcctcgcgt cccggaagat gaatacgcag
    22321 atccagaccg ctgttcagtt cattcataaa ctgacggcag tgaccacaag gcgtgtagtt
    22381 aacggtgatg gctgcaagcg ctttttcacc actcagccag gcgtggctga tcgcgctttg
    22441 ttcggcatga acggtttgct gcattgtcgc accaataaat tccatattgg caccgaaata
    22501 ccaggttccg ctcacaccgc gcgcaattgc gccaacatta aaattcgaca atggcgtacg
    22561 cgcacaggcc gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt ccagccccgt
    22621 tgcgctcttc agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc
    22681 cagaataggt tccagtgcag attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa
    22741 acgtggatgc atggcgttgc ctcataatca tgtaatggga taacagttta cgaacccgct
    22801 atggctttat atgtgatctg aatctcatta attatgcaac gcgtgaaact tacatcaaaa
    22861 atgcgcgacg catcgcaaat tttagcccat tactgccaga ataatcggga aaaggaacgg
    22921 tgcgatcagc gaagtaataa tcccgcataa caccagcgcc agcgaactaa atgcaccttc
    22981 ctgataatcc agctcggcgc agcgcgccgt accgagggcg tgcgaggctg tccccattgc
    23041 cagaccgcgc gcagctttgg tacgaatacg catcgcatta agcaatgtat gaccaaacac
    23101 agcgccgagg atgccgacga aaatcacgca aactgcgctg attgccggaa taccgccaat
    23161 actgccgcca actgccattg caataggcgt ggtgactgat ttcggcagga ttgacgcggc
    23221 gatttccggt gaagcgccca tcaacaatgc cacagaagtc cccgtcacca tcgcaaccac
    23281 gctgccgata aaacagatgg tgatgatcga tttccagcgc gcgcggatct ggtgtagctg
    23341 ctcatataaa ggataggcca gtgcgacgac cgccggttgc agcagatcgt ttagcacttc
    23401 gctaccttta aagtagctgt cgtaagagat gccggtcagc attaaaaaag gaatgatcac
    23461 caccattgct accagcaacg ggttaagcag gggaaactta taccgtgccg ccagtttgcg
    23521 ggcggcaaaa aagacaatca acgtcagcgg taatgaccac cagatattcg ccatcattct
    23581 tctgatcctt tctgacctac gactttacgt tcaccatgca ccagttgtga actccagctc
    23641 accaccagaa aaaccaccag cgtactgact gcacaggaaa ccactaccgg accaaactgt
    23701 gcgcggagca aatcaaaata ttgcatgacg cctacaccaa tcggcacaaa caatagcgcc
    23761 atatagcgaa tcagtacgta gcaccccgga ttgacccatt tcgccggaag aatttgcaag
    23821 gccagcagga caaacaggat cagcatcccg atgatgctgc ccggaatggt taccggtagc
    23881 agggaagcaa tgaaaatgcc tgcatacagg caggcataaa tcaggacaaa agcacgtaaa
    23941 tattgccaga taatgttcag tgtcttactc ataatcaaca ccccagatga gatggattca
    24001 tcatacaatt aattcgtaaa acgtgctacc gatcacatta agaaatcagc gcttattttt
    24061 tcgctccccg gtattacgca atgcgcagcg aactcaggca tacatcaagc atagagaggg
    24121 attgcagttt gcagcaatcc ctctttgcag acctttaatc cgttgaccgg aggcgctaac
    24181 atagcgcctc atttttttgc gggtgatgat atgcgtgtgt tactggctcc gatggagggt
    24241 gtacttgact ctctggtgcg ggaattactg accgaagtta acgactacga tctgtgcatc
    24301 accgagtttg tccgcgtggt ggatcaactg ctgccagtaa aagtctttca tcgcatttgc
    24361 cctgagctac aaaatgccag ccggacacca tccggtacgc tggttcgcgt gcagttgtta
    24421 gggcagttcc cacagtggct ggcagagaac gccgcccgtg cggtggagtt aggttcctgg
    24481 ggcgtggatc ttaactgcgg ctgcccgtcg aaaacggtta acggtagcgg tggtggggca
    24541 acgttactga aagatcctga actcatctac cagggtgcaa aagcgatgcg cgaagctgta
    24601 ccggcgcatt tgcctgtcag cgtaaaagtg cgtctgggct gggacagcgg tgagaagaaa
    24661 tttgaaatcg ccgatgcggt tcaacaggct ggtgctacgg agctggtagt acatgggcgg
    24721 acaaaagagc agggttaccg cgcggaacat attgactggc aggcgattgg cgagattcgc
    24781 cagcggctta atattccggt gattgccaac ggtgaaatct gggactggca gagtgcgcaa
    24841 caatgcatgg cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc gctcaatatt
    24901 cccaacctga gccgtgtggt gaaatataac gaaccgcgaa tgccgtggcc ggaggtggtt
    24961 gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt atatcacgtt
    25021 gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc aacggaatta
    25081 tttcagcatg ttcgggtgtt gaataattcc cctgatattg caagggctat tcaggccatt
    25141 gatatcgata agctgcgata atagtggaat cgcagcttat ctgagacaat gaatcgctta
    25201 ttcagcttca tcatcttgtt gcgctccttt catgagctaa gccacatatt gccactggcg
    25261 caaggagcgc gcagggggag gacaatcgcc tcctccccct gcacccccgg gctctggcga
    25321 acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggctaccgg gtcgggcgcg
    25381 aggtaacgtc cttgtaagac gcgccctcag cccacatcca tgtgggctgc cccggccttc
    25441 ggggaacgct tcagcgattt tgacgccacc aacaccggag ctgtttgcaa atcaagtaca
    25501 caaaatatct agttcctgac ctgacaactt atatgccata tctgattttt aaaatatcat
    25561 cttaaatacg ccagtcacca ccagtagccc aatcaggaaa ataatcaata cagcccagag
    25621 tataattttc attggcgttc ctttatttgt gagtgctgaa taagtatagg aaaaaacgaa
    25681 gccctttgcc gctatccata aacgattaag ataaatcgga agatatcatt cacttagtgt
    25741 atatttttct ggatatgtcg ttttattgct actgatgtat tgccacgcga aactaaaaaa
    25801 tatatcgcaa cacacggtaa tctccgcgca ccttttctta cctgattatt cccccatgaa
    25861 tcgtgattcc ttttatcccg ccatcgcctg ttttccgctg ttactgatgc tggccgggtg
    25921 tgcacctatg catgaaaccc gccaggcgtt aagccagcaa acgcccgctg cacaagttga
    25981 caccgcatta cccacggcgc tgaaaaatgg ttggccagac agccaatggt ggctggagta
    26041 tcacgataat caactcactt ccttaattaa caatgcacta cagaacgcac cggatatgca
    26101 ggtggctgaa cagcgcattc aactggctga agcccaggcg aaggcagttg ctactcagga
    26161 tggtccgcag atcgactttt ctgcggatat ggagcggcaa aaaatgtctg cagaagggtt
    26221 aatggggccg tttgctctga acgatcctgc cgcaggtacg accggtccgt ggtacaccaa
    26281 cggtactttt ggcttaacgg cgggctggca tctcgatatc tggggaaaga atcgggcaga
    26341 ggtaactgct cgcctgggta cggttaaagc acgagcggcg gaacgcgagc aaacccgcca
    26401 attgctggct ggcagcgtag cccgcctgta ctgggagtgg caaacccagg cggcgttaaa
    26461 cactgtctta cagcaaatcg aaaaagagca gaacaccatt atcgccaccg atcgccagct
    26521 atatcagaac gggattactt cttcagttga aggtgtggaa accgatatta atgccagcaa
    26581 aacccggcag cagctcaacg atgtcgcggg gaaaatgaaa attattgagg cacggttaag
    26641 cgcacttaca aatcaccaga caaaggcatt gaagcttaat ccggtcgcgt tgccgaaagt
    26701 ggcaagtcag cttcctgatg aactggggta ctccttactg gcccggcggg cagatttgca
    26761 ggcggcgcac tggtacgttg agtcatcgtt aagcactatt gatgcggcaa aagcggcatt
    26821 ttatcctgac atcaacctga tggccttcct gcaacaggat gcgttgcact taagcgatct
    26881 gttccgtcat tccgcgcagc aaatgggcgt tacggcaggc ctgacgctac ccattttcga
    26941 tagtggtcgt cttaacgcca acctcgatat cgcaaaagcc gaaagcaact tgtctatcgc
    27001 cagctacaac aaagcggtgg ttgaagcggt gaatgacgtg gcgcgggcag ccagtcaggt
    27061 tcagacactg gcggagaaaa accagcatca ggcgcaaatt gagcgcgatg ccttgcgtgt
    27121 agtaggtctt gcgcaggcgc gctttaacgc gggcatcatt gcgggttccc gcgtcagcga
    27181 agccagaatc cccgcgctgc gtgagcgggc caatggcctg ttattgcaag ggcagtggct
    27241 ggatgcctcc attcaactca ctggtgcgtt gggcgggggt tacaaacgct gatgatatgc
    27301 gccttctata cttaacgttt attcagcgtt aagtggagaa ctcgatggca caggtcgcga
    27361 ttattaccgc ctccgattcg gggatcggca aagagtgcgc gttattactg gcgcagcagg
    27421 ggtttgatat tggcattacc tggcactcag atgaagaagg ggcaaaagat accgcgcgtg
    27481 aggtagttag ccacggcgta cgtgcggaga tcgtgcagct ggatctcggc aatctaccag
    27541 aaggggcact ggcgctggag aaactcattc aacggctggg gcgtattgat gtgctggtga
    27601 ataatgcggg cgcaatgacc aaagcgccgt ttcttgatat ggctttcgat gagtggcgca
    27661 agatttttac cgttgatgtc gatggtgcat ttttatgctc gcaaattgct gcccgccaga
    27721 tggtgaaaca agggcagggt ggtcgcatca tcaacattac gtcggtgcat gaacatacgc
    27781 cgttgccgga tgccagcgcc tacacagccg ctaaacatgc gctcggcggg ttaaccaaag
    27841 cgatggcgct ggagctggtc aggcataaga ttttggtgaa tgcggttgca cctggggcga
    27901 tcgccacgcc aatgaacggc atggatgatg gcgacgtgaa gcccggcgcg gagccttcga
    27961 ttcccttgcg gcgttttggc gcaacgcatg agattgccag cctggtggtg tggctttgtt
    28021 cggagggcgc aaattacacc actgggcagt cgttgatagt ggatggcggc tttatgctgg
    28081 cgaatccgca gttcaaccca gaatagcgca gtgaggtgcg tggttatttc aatgggtaat
    28141 atttgggtag gcatgataag acgcacaagc gtcgcatcag gcatggtgca ccggatgtcg
    28201 gatgcggcgc gaacgcctta tcctaggcaa tggttttacg cctgattatc cggctttttc
    28261 ttcccgcgtc gtttcagcca ccagcgcacg cctaccacca gaaccacgac cagaatcaac
    28321 cagacccagt gcttcaaatg ctggtcgaga ttatgcaacc acggcgcaat cacctgacca
    28381 ccagcgtaac cgatagtggt aaaaatcaac gcccaggcaa atgcgccgag gatattcagt
    28441 ggcagaaaga ttttcggcgg cagctggctg gcaccaatca gcgtcgggcc aatgacccga
    28501 aagccataca taaaacgcgt accaatgaca aacagatacg gatggcgttg gatcagtttc
    28561 tgcgcccgct caattttatc ctgatgtttc gagaaacggc gtaatagctt gccgccaaac
    28621 cgccgcccgc acagataaag cacctggtcg ccaatcatgc cgccaagcgc cacagaaagt
    28681 accaccagcg ggaactttaa tagcccctga tgtgccgcaa cgcctcccag caaagtcacg
    28741 gtttcacctt ccgccaggct accgatcacc agcgcggcat aaccatattg tgagatgagt
    28801 gtattgagat ccataggaca gtctgtctcc ctttgcttgc ctgtaagcat acaccctgtg
    28861 aatcattcac gctgaaaaag gacgtacatc agacggtgca aattacatga gtgaacaaaa
    28921 aataatcaca agctgactta tacttgaact gtcaggttca tgaactcaca acaagggggc
    28981 ggtatgagcc atgtctgggg actgttttcc catcccgatc gtgaaatgca ggtgattaat
    29041 cgcgaaaacg aaacgatttc tcatcactac acccaccacg ttttgctgat ggcggcgatt
    29101 ccggtgattt gcgccttcat tggcactaca cagattggct ggaattttgg cgatggcact
    29161 atcctgaagt tatcctggtt taccggactg gcgctggctg tcttgtttta cggcgttatg
    29221 ctggctgggg ttgcggtgat ggggcgggtc atctggtgga tggcgcgtaa ttatccgcag
    29281 cgtccgtcac ttgcgcactg catggtcttc gcgggctacg ttgcaactcc gctgttttta
    29341 agcggtctgg tggcgcttta cccgctggtc tggttgtgtg cgctggtcgg cacggtggcg
    29401 ctgttttaca ccggctacct gctgtatctc ggtattccga gcttcctgaa tatcaacaaa
    29461 gaggaaggac ttagcttctc cagttcgaca ctggcgatag gcgtgctggt gctggaagtg
    29521 ctgctcgccc ttaccgttat actatggggc tacggttatc gtttgttcta gaaagttctt
    29581 ttgcgctgca ttgctggcgt aaatgtcagc aatgcagcat ttcctcacga ttctcctttg
    29641 acgatcgcac tttttgctcg ttatgatggc gtcgctagcc tcagtaaatc cttactccgg
    29701 cggtgcgcaa cccgtgcgcg tgaaccacta tctgaatgct catcatgccg aaatttcgag
    29761 tttctttatt tagcctggcc ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga
    29821 aaacggcagc cgctaccacc gcgtcacaac cggaaattgc ctccggtagc gcgatgattg
    29881 ttgatctgaa taccaacaaa gtgatctatt cgaaccaccc ggatctggtg cgtccgattg
    29941 cgtctatcag caaattaatg accgcgatgg ttgtgctgga tgcacgactg ccgctggatg
    30001 aaaaactaaa agtggatatc agccagacgc cggagatgaa aggggtctat tcgcgcgtac
    30061 gactgaacag cgaaatcagc cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa
    30121 accgcgcggc ggcaagcctt gcgcaccatt atcccggtgg ttacaaagcc tttattaagg
    30181 cgatgaatgc gaaagcgaaa tcgctcggaa tgaacaacac gcgctttgtt gaaccaaccg
    30241 gattgtcggt gcataacgtt tcaaccgccc gtgacttaac caaactactc attgccagca
    30301 aacaatatcc gttgatcggg cagttaagta ctacccggga agatatggca accttctcta
    30361 acccgacgta tacgctgccg ttccgcaata ctaaccatct ggtgtatcgt gataactgga
    30421 atattcagtt aaccaaaacc ggttttacca atgcggcggg ccattgtctg gtgatgcgca
    30481 cggttatcaa taataaaccg gtggcgctgg tagtgatgga cgcgtttggc aaatataccc
    30541 attttgccga tg
//
LOCUS       DAOFSV010000044        30389 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.044, whole genome shotgun sequence.
ACCESSION   DAOFSV010000044 DAOFSV010000000
VERSION     DAOFSV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.044"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            39..1472
                     /locus_tag="QBH27_002107"
     CDS             39..1472
                     /locus_tag="QBH27_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ7014417.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            1606..2676
                     /gene="ygjJ"
                     /locus_tag="QBH27_002108"
     CDS             1606..2676
                     /gene="ygjJ"
                     /locus_tag="QBH27_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDQ7014418.1"
                     /translation="MKLITAPCRALLALPFCYAFSALGEEARPAEHDDTKTPEMISTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            2693..5044
                     /gene="ygjK"
                     /locus_tag="QBH27_002109"
     CDS             2693..5044
                     /gene="ygjK"
                     /locus_tag="QBH27_002109"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDQ7014419.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPMQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIIERGKGPESWSPLFNGEATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGSQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            5270..7288
                     /gene="fadH"
                     /locus_tag="QBH27_002110"
     CDS             5270..7288
                     /gene="fadH"
                     /locus_tag="QBH27_002110"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDQ7014420.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(7333..7749)
                     /gene="higA"
                     /locus_tag="QBH27_002111"
     CDS             complement(7333..7749)
                     /gene="higA"
                     /locus_tag="QBH27_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDQ7014421.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(7746..8060)
                     /gene="higB"
                     /locus_tag="QBH27_002112"
     CDS             complement(7746..8060)
                     /gene="higB"
                     /locus_tag="QBH27_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HDQ7014422.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(8345..9481)
                     /gene="rlmG"
                     /locus_tag="QBH27_002113"
     CDS             complement(8345..9481)
                     /gene="rlmG"
                     /locus_tag="QBH27_002113"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDQ7014423.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            9566..10069
                     /locus_tag="QBH27_002114"
     CDS             9566..10069
                     /locus_tag="QBH27_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDQ7014424.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            10146..10838
                     /locus_tag="QBH27_002115"
     CDS             10146..10838
                     /locus_tag="QBH27_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDQ7014425.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            10917..11903
                     /locus_tag="QBH27_002116"
     CDS             10917..11903
                     /locus_tag="QBH27_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ7014426.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNQAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            12186..13151
                     /gene="alx"
                     /locus_tag="QBH27_002117"
     CDS             12186..13151
                     /gene="alx"
                     /locus_tag="QBH27_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDQ7014427.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            13475..14719
                     /gene="sstT"
                     /locus_tag="QBH27_002118"
     CDS             13475..14719
                     /gene="sstT"
                     /locus_tag="QBH27_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDQ7014428.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(14723..15274)
                     /locus_tag="QBH27_002119"
     CDS             complement(14723..15274)
                     /locus_tag="QBH27_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDQ7014429.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(15357..16844)
                     /gene="uxaA"
                     /locus_tag="QBH27_002120"
     CDS             complement(15357..16844)
                     /gene="uxaA"
                     /locus_tag="QBH27_002120"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDQ7014430.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(16859..18271)
                     /gene="uxaC"
                     /locus_tag="QBH27_002121"
     CDS             complement(16859..18271)
                     /gene="uxaC"
                     /locus_tag="QBH27_002121"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDQ7014431.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            18754..20052
                     /gene="exuT"
                     /locus_tag="QBH27_002122"
     CDS             18754..20052
                     /gene="exuT"
                     /locus_tag="QBH27_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDQ7014432.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            20182..20958
                     /gene="exuR"
                     /locus_tag="QBH27_002123"
     CDS             20182..20958
                     /gene="exuR"
                     /locus_tag="QBH27_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDQ7014433.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            21303..21965
                     /locus_tag="QBH27_002124"
     CDS             21303..21965
                     /locus_tag="QBH27_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ7014434.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKFEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            21969..22352
                     /gene="mzrA"
                     /locus_tag="QBH27_002125"
     CDS             21969..22352
                     /gene="mzrA"
                     /locus_tag="QBH27_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDQ7014435.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            22499..22867
                     /locus_tag="QBH27_002126"
     CDS             22499..22867
                     /locus_tag="QBH27_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDQ7014436.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            22905..23210
                     /locus_tag="QBH27_002127"
     CDS             22905..23210
                     /locus_tag="QBH27_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDQ7014437.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            23213..23617
                     /locus_tag="QBH27_002128"
     CDS             23213..23617
                     /locus_tag="QBH27_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDQ7014438.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            23607..23906
                     /locus_tag="QBH27_002129"
     CDS             23607..23906
                     /locus_tag="QBH27_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDQ7014439.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            24093..24485
                     /locus_tag="QBH27_002130"
     CDS             24093..24485
                     /locus_tag="QBH27_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDQ7014440.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            24555..25541
                     /gene="yqjG"
                     /locus_tag="QBH27_002131"
     CDS             24555..25541
                     /gene="yqjG"
                     /locus_tag="QBH27_002131"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDQ7014441.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGASGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTINNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            25824..26192
                     /locus_tag="QBH27_002132"
     CDS             25824..26192
                     /locus_tag="QBH27_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ7014442.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(26226..27122)
                     /gene="yhaJ"
                     /locus_tag="QBH27_002133"
     CDS             complement(26226..27122)
                     /gene="yhaJ"
                     /locus_tag="QBH27_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDQ7014443.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            27227..27928
                     /locus_tag="QBH27_002134"
     CDS             27227..27928
                     /locus_tag="QBH27_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDQ7014444.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            27951..28115
                     /locus_tag="QBH27_002135"
     CDS             27951..28115
                     /locus_tag="QBH27_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014445.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(28176..29486)
                     /locus_tag="QBH27_002136"
     CDS             complement(28176..29486)
                     /locus_tag="QBH27_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDQ7014446.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(29514..>30389)
                     /locus_tag="QBH27_002137"
     CDS             complement(29514..>30389)
                     /locus_tag="QBH27_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein"
                     /protein_id="HDQ7014447.1"
                     /translation="SDSPFYGLVLICILVAISSRGEKLLFKISTGMVLTKLLVVAALG
                     VSMVGMWHLYNVGSLPPLGLLMKNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEV
                     ARHKALRAMNIAFGILFVTVFFYAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDG
                     AAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILRRKIPAEKINENLVQRGIM
                     IFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLII
                     VTGLLLCVSPFLAFS"
BASE COUNT         7176 a         7904 c         7888 g         7421 t
ORIGIN      
        1 gccgccaccg ctaccctact cattttcgga gatgtgttat gtctgatacc aaacgtaata
       61 caatcggcaa attcggcttg ctctcgctga cttttgccgc cgtttacagc tttaacaacg
      121 ttatcaataa taatattgag cttggactgg cctcggcacc gatgtttttc ctcgcaacaa
      181 ttttttattt tattcccttc tgtctgatca tcgcagaatt tgtttcgtta aataaaaact
      241 cggaagccgg tgtctacgcg tgggtaaaaa gttcgctggg cggacgttgg gcatttatta
      301 ctgcctatac ctactggttc gtaaacctgt tctttttcac ctcattattg ccgcgcgtta
      361 ttgcttacgc ctcgtatgca ttcctcggct acgaatatat tatgacgccg gttgccacca
      421 ccattatcag tatggtgctg ttcgcctttt ccacctgggt ttctaccaac ggggcgaaaa
      481 tgctggggcc aattacctcc gtcacttcaa cgctgatgct gctgttaacg ctctcctaca
      541 ttttactggc aggtacggca ctggttggcg gcgtacagcc tgctgacccc atcactgttg
      601 acgcgatgat cccaaacttt aactgggcgt tcctcggcgt taccacctgg atctttatgg
      661 ctgcaggtgg cgcggagtcc gtcgcggtgt acgttaacga cgtcaaaggc ggttcgaaat
      721 cgttcgttaa agtgatcatc ctcgccggga tttttattgg cgtactgtac tccgtctcct
      781 cggtactgat taacgtcttc gtcagcagca aagagttgaa gtttaccggc ggatcggtac
      841 aggtattcca cggcatggcg gcgtattttg gtctaccgga agcactgatg aatcgctttg
      901 tcggtctggt gtcctttacc gcgatgttcg gttccctgct gatgtggacg gcaacgccgg
      961 tgaaaatttt cttctccgaa attccggaag gcatctttgg taagaaaacc gtcgaactga
     1021 acgaaaacgg cgttccggcg cgcgcagcgt ggatccagtt cctgatcgtc atcccgctga
     1081 tgattatccc gatgctcggt tccaataccg tgcaggatct gatgaatact attattaata
     1141 tgactgctgc agcgtccatg cttccgccgt tattcatcat gctggcttac ctgaatttac
     1201 gcgccaaatt agatcacctg ccacgcgatt tccgtatggg ctcccgccgc accggtatta
     1261 tcgttgtttc aatgctgatt gcgatatttg cagtagggtt tgtcgcttcg acattcccga
     1321 ctggcgcgaa tattctgacc atcatttttt ataacgtcgg cggtattgtt atcttcctcg
     1381 gctttgcgtg gtggaaatac agtaaatata taaagggatt aacggctgaa gagcgccata
     1441 ttgaagcgac gccagccagc aatgttgatt aagcagaata ataaaaaatg agcaggcatt
     1501 ttccctcttc ctggatgggg agagggaaaa tagtttctgt ctttatattt ttataattac
     1561 aacgataaaa ggctgtactt tttctttagc tcatggatta acacaatgaa attaatcact
     1621 gcaccatgca gagcattact tgccctgcct ttttgctacg ctttttctgc acttggcgaa
     1681 gaagcacgtc cggcagaaca tgacgacaca aaaacccccg aaatgatctc gacatcttct
     1741 ccttcatttc gtttttacgg cgaattaggg gttggtgggt atatggattt agagggtgag
     1801 aataaacata aatacagcga cggcacctat attgaaggtg gcctggagat gaagtacgac
     1861 tcctggttcg gcctgattta cggcgaaggc tggaccgtgc aggccgacca cgacggtaat
     1921 gcctgggtgc ctgaccatag ctggggtggt ttcgaaggtg ggattaaccg tttctatggc
     1981 ggttatcgta ccaatgatgg caccgaaatc atgctcagtc tgcgtcagga ttcctcgctg
     2041 gatgacctgc aatggtgggg cgatttcacg cccgatctgg gctacgtcat tcccaatacc
     2101 cgcgacatta tgactgcgct gaaggtacag aacttaagcg gcaactttcg ttatagcgtt
     2161 accgccacac ctgccggaca tcatgacgaa agcaaagcct ggctacattt tggcaagtac
     2221 gatcgctacg acgacaaata cacctatccg gcaatgatga acggttacat ccagtatgac
     2281 cttgcagaag gtatcacctg gatgaacggt ctggaaatca ccgacggcac aggccaactc
     2341 tatctcacgg gcctgcttac ccctaacttt gccgctcgcg cctggcacca taccggacgc
     2401 gccgacgggc tggacgtacc gggaagtgaa agtgggatga tggtgagcgc catgtatgaa
     2461 gcgttaaagg gcgtttatct ctccaccgct tacacctacg ccaaacatcg ccctgaccac
     2521 gctgacgatg aaaccacctc tttcatgcag tttggtatct ggtacgaata cggcggcgga
     2581 cgtttcgcaa cggcttttga tagccgcttc tacatgaaaa atgcctctca cgatcccagc
     2641 gaccaactct tcctgatgca atatttttac tggtaataag gacagtaatc tcatgaaaat
     2701 caaaactatt ttaacaccag tagcctgtgc tctgctgatg agcttttccg cccatgccgc
     2761 taacgctgac aattataaaa acgtgattaa ccgtactggc gcgccgcagt acatgaagga
     2821 ttacgattac gacgatcacc agcgttttaa tccgtttttc gatctcggag cctggcatgg
     2881 tcatctgttg ccagacggcc cgaacactat gggcggcttt ccaggcgttg cgttgttgac
     2941 ggaagagtac atcaacttta tggccagcaa tttcgaccgc ctgaccgtct ggcaggacgg
     3001 caagaaagtc gatttcacgc tggaggcata cagtattccc ggcgcgctgg tgcaaaagtt
     3061 gatatcgaaa gatgtacagg tcgaaatgat tctgcgcttc gccacgccgc gcacatcact
     3121 actggaaacc aaaatcacca gcgataaacc gctggatctg gtgtgggatg gcgaactgct
     3181 ggaaaaactg gaagcgaaag aagggaaacc gctttccgat aaaaccattg ctggcgagta
     3241 ccctgactat cagcgcaaaa tcagcgccac ccgcgatggc ctgaaagtta cctttggcaa
     3301 agtgcgcgcc acctgggatc tgctgacctc cggtgaatca gaatatcagg tgcataaatc
     3361 attaccgatg cagactgaaa tcaacggcaa tcgctttacc agtaaggcgc atatcaacgg
     3421 atcgacgacg ctctatacca cctattccca tctgctgacc gctcaggaag ttagcaaaga
     3481 gcaaatgcag atccgcgata ttctggcgcg tccggcgttt tatctcaccg cctcgcagca
     3541 acgttgggaa gaatacctga agaaaggatt aaccaatccg gatgcgacgc cggaacagac
     3601 gcgcgtcgcg gtgaaagcca tcgaaacgct caacggtaac tggcgatcgc cgggcggtgc
     3661 ggtgaaatat aacaccgtca cgccgtcggt gaccgggcgc tggttctccg gcaaccagac
     3721 ctggccgtgg gatacctgga agcaggcgtt tgcgatggcg catttcaatc cggacatcgc
     3781 caaagagaat atccgcgcgg tcttctcctg gcagatccag ccaggcgacc gcgtgcgtcc
     3841 gcaggatgtg ggctttgtcc ccgacctgat agcgtggaac cttagccccg agcgtggcgg
     3901 cgatggcggc aactggaacg aacgtaatac caaacccagc cttgccgcct ggtcagtaat
     3961 ggaagtgtat aacgtcaccc aggataaggc ctggttggca gagatgtacc cgaagctggt
     4021 ggcctatcac gactggtggt tacgtaaccg cgatcacaac ggcaacggcg tgccggaata
     4081 tggcgcgacc cgcgacaaag cccacaacac cgagagcggc gagatgctgt ttacggtgaa
     4141 aaaaggcgac aaagaggaga cgcagtctgg gctgaacaac tacgcccgtg tggtggagaa
     4201 aggccagtac gacagtctgg aaattccggc acaggttgct gcgtcgtggg aatcaggtcg
     4261 tgatgacgcc gccgttttcg ggtttatcga caaagaacag ctggataaat atgtcgctaa
     4321 cggcggcaaa cgtagcgact ggacggtgaa attcgccgaa aaccgcagtc aggacggaac
     4381 gttgctgggc tactcgctat tgcaggagtc ggtggatcag gccagctata tgtacagcga
     4441 taaccattat ctggcggaga tggcgacgat tctcggtaag ccggaagagg ccaaacgcta
     4501 tcgtcagtta gcacaacagc tcgcggacta catcaacact tgtatgttcg acccgactac
     4561 acagttctac tatgacgtgc gtattgaaga taaaccgctg gcgaacggct gcgcgggcaa
     4621 accgattatt gagcgcggta aagggccgga aagctggtcg ccgctgttta acggtgaggc
     4681 aacgcaggcc aatgccgacg cggtggtgaa ggtgatgctg gatccgaaag aattcaacac
     4741 ctttgtcccg ctgggaacgg cggcgttaac caatccggct tttggcgcgg atatctactg
     4801 gcgcgggcgc gtatgggtgg atcagttctg gtttggtctg aaagggatgg agcgttacgg
     4861 ttatcgtgat gatgccctga agctggcgga tacgttcttc cggcacgcca aaggattaac
     4921 cgccgatggc ccaattcagg agaattacaa cccgctcact ggctcgcaac aaggcgcacc
     4981 aaatttctcc tggagtgccg cgcatttgta tatgttgtat aacgattttt tccgtaagca
     5041 gtaatttgca acggctggcg gatgcggcgt gaacgcctta tccgccctac atgtgcaatc
     5101 ccccaggccg gataaaacgc ggcaagcgtc gcatccggcg ttaccaccgg gcgtattctt
     5161 tttgaatccc atcacaaacc ccgcactccc cttttccctt ttctccggcg acggctaaat
     5221 tagaactcat ccgaccacat aacaattatt ttacatactg gacaatttta tgagctaccc
     5281 gtcgctgttc gccccgctgg atttaggttt taccacgtta aaaaaccgcg tgttgatggg
     5341 ctcaatgcac accgggctgg aggagtaccc ggacggcgct gaacggctgg cagcgtttta
     5401 tgccgaacgc gcccgtcacg gcgtggcgtt gattgtcagc ggcggtatcg caccagattt
     5461 aacaggcgtt ggcatggaag gcggtgcaat gctcaatgac gccaaccaga tcccacacca
     5521 tcgcaccatt accgaagccg tacatcagga aggcggcaaa atcgccctgc aaattttgca
     5581 taccgggcgc tacagctacc aaccgcatct ggtcgctccc tccgcattgc aggcccccat
     5641 caaccgtttc gttcctcacg aactcaccca tgaagagatc ctgcaactga tcgacgattt
     5701 cgcccactgc gcgcaattgg cacgggaggc aggatacgac ggtgtagagg tgatggggtc
     5761 cgaagggtat ttgatcaacg aatttctgac gctgcgcacc aatcagcgta gtgaccagtg
     5821 gggcggcgat taccgcaacc ggatgcggtt tgccgtagaa gtagtgcgtg cggtgcgcga
     5881 acgtgtcggc aacgacttca ttattatcta ccgtttgtcg atgctcgacc tggtcgaagg
     5941 cggcggtact tttgccgaaa tggtagagct ggcgcaggcc attgaagcgg cgggcgcgac
     6001 cattatcaac accggcattg gctggcatga agcgcggatt ccgaccatcg ccacgcccgt
     6061 gccgcgcggc gcctttagct gggtcacgcg caaactgaaa gggcacgttt cgctgccact
     6121 ggtgaccacc aaccggatta acgatccgca ggttgccgac gacattctct cgcgcggtga
     6181 tgcggacatg gtatcgatgg cgcgaccgtt tctcgctgat gcggagctgc tatcaaaagc
     6241 gcaatcggga cgagccgatg agatcaacac ttgtattggc tgcaaccagg cctgtctcga
     6301 tcagatcttc gttggcaaag tcacctcgtg cctggtgaat cctcgcgcct gccacgaaac
     6361 caaaatgcca atccttcctg ccgtgcagaa aaaaaatctg gcggtggtcg gtgcgggacc
     6421 tgctgggctg gcgtttgcca ttaacgcggc ggcgcgtggg catcaggtaa cattgtttga
     6481 tgcgcacagc gagatcggcg ggcagtttaa tatcgccaaa cagatccccg gcaaagagga
     6541 gttttacgaa acgctgcgct attaccgccg gatgatcgaa gtgacgggtg taacgctaaa
     6601 actcaatcac accgtgacgg cggatcagtt acaggcgttc gatgaaacga tcctcgccag
     6661 tgggatcgtg ccgcgcgttc cgcctatcga cgggatcgat catccgaagg tactgagtta
     6721 tctcgatgta ctgcgcgaca aagcgccggt gggcaacaaa gttgccatca tcggttgtgg
     6781 cggaattggt tttgatacgg cgatgtattt aagtcagccg ggcgaatcca ccagccagaa
     6841 tatcgccggg ttctgtaatg agtggggaat cgacagtagc ctgcaacagg ccggaggttt
     6901 gagtccgcaa ggcatgcaaa tcccccgtag cccacggcag attgtgatgc tccagcgcaa
     6961 agccagcaaa ccaggacaag ggttaggcaa aaccaccggc tggatccatc gcaccaccct
     7021 gctctcacgc ggcgtgaaaa tgatcccagg cgtaagttac cagaagatcg acgatgacgg
     7081 gctgcatgta gtgatcaacg gcgaaacgca ggtactagca gtggacaatg tggtgatctg
     7141 cgcggggcaa gagccaaacc gcgcattggc gcaaccgctg attgatagtg ggaaaactgt
     7201 gcatttaatt ggcggctgcg atgtagctat ggagctggac gcacggcggg caattgccca
     7261 gggaacacgg ctggcgctgg agatttaaat cgttgtttga agcgccggat gcgacgcatc
     7321 cggcacgtcg cgttaatcaa taaacaaggc gggagaaata ccgaatcgcg ttgccagttt
     7381 tttagcgtgt tccagcgtta atttcctttt cccgctcaaa acgcgtgaca ccatcgattt
     7441 actgcctatt tccggaagat cggaaagggt taaaccatat tgatccataa gggtacgaat
     7501 cacggctata ccgccaggca tggcttgagc catggcatta aattccacaa attcgggcgc
     7561 tgattcttcc cacgcggtta ttttggcaca caccagatcc agtaaggggt tttcaggatc
     7621 gttgagcagc agatgatcta ccagttccag cgcctgggtg tattgttcct cgttctgaat
     7681 acccgccaga aaaggtgcca cagcagttag cttttctcct gcttgcaaga tgtcggcaat
     7741 cgcaatcatt ttttcccctt agtacgatga acggcggtaa agaaatcgta ttctttatgc
     7801 gtcataactt cacgtatgta gcacttttgc gattcaaaaa agaccattgc tacaacacgt
     7861 aattcattgc ccccgacatt gaaaacataa tgcttatcca gatatttgaa gttatccaga
     7921 gaagggaata ctgcttttaa tgactcaggt tttttgaaat atcccttagc aatcgtgttc
     7981 cccagagcca ccaactccgt tttatgttgc gggtattttt ccgcagcatc tttcaatgct
     8041 ttttgagtta tcaggtgcat tcttcatcac gtccgttgac aaattggcaa tatgataaca
     8101 tccgttgaca gattggcaac gaatgaattt atctgtgtga cggaagaata aacgtagaaa
     8161 aacgctaaac aagagaatgt agaggatggt gacaggaatt ttttgtttta tggaagcgcg
     8221 attccagaac tgggcagaca ttgccggatg cgacgcttgc cgcgtcttat ccgacctggg
     8281 ggtaatgtgc tgaacgtatg ccggataagg cgtttacgcc gcatccggca ggtaaccctc
     8341 aatcttaccg acgacgcccc agcttcactg ctttcagcac cacaaattta ttattcgtgg
     8401 cgatggtggt gcagttgccg aaaattttct tcagtttatg gaagtaatcc aagtgacggt
     8461 tggcaacgat atacagctcg ccgttgattt tcaggcagcg gcgggcgtgg tggaacatct
     8521 cccaggcgac gttatcggtc agcgcatgtt gctgatgaaa cggcgggttg cagagcacag
     8581 cattaaaacg gaaaggctcc acgccggaaa gcgcgttgtt gatcataaac tcgcagcgat
     8641 ccagtgcctc gggcatgttg gtttcaacgt tcaaacggct ggaagcaacc gccatcggtg
     8701 attcatcgac aaacaccact ttcgcctgcg ggtttttatc gagcaatgtc aggccaataa
     8761 cgccattacc gcaaccgaga tcgacaatct caccttcgag attctctggc agatgttgca
     8821 taaagaagcg cgcgccaata tccagcccgg tgcgggagaa gacattcgca tggttgtgga
     8881 tagtccagtc agtaccttcc agcttccagc taacggtctg cggcgcatcg gccaacggcg
     8941 gttcgttgaa ggtgcagtta atcaggcgcg ctttcttcca tgccagcgtg gtggtggtcg
     9001 ggccgagcac tttttcgaac aattccagcg tggaagtgtg aatgtcacgg gctttggcac
     9061 cagcaataat gcgcgtatct ggcgtgacca ctttgcgcag cgcgcgcagt tgttgttcca
     9121 gcaatgccag tgttttcggc actttgatca gcaccacacc cggctgttgc gggtagtcgg
     9181 cggtgctgtc gagaaacttc acgctcgact catcaatccc gttgaggcgt aaattctcgc
     9241 gcgtggccag ttcactgatg tatgagtcgc caatgctgta cggcttatgt tccgccagca
     9301 cacagcttaa cgcaccaaaa gcatcattca ggatcaacac cgggccgcgg atttctgtgt
     9361 cgtccaactg ttgcagcaaa tactcatccg ccgcttccca cgcctgtagc gggttaacgt
     9421 catccgtcgc cggaaaacgt tgtagtgtca gtgaacggaa accgttgtct aagtggctca
     9481 tcggccctcc tgaatgataa aatttcgacg ttgcccctga aaagggtgcg tgagtatacc
     9541 ctttttctct ttttcgtggg cggttatgag caatcttact tatcttcagg gttatcccga
     9601 gcagctactt tcccaggtgc gaacgctgat taacgaacag cgtctgggcg atgtgctggc
     9661 aaaacgttat ccgggaacac acgactacgc caccgataaa gccctctggc aatatactca
     9721 ggatcttaaa aatcagtttc tgcgcaatgc cccgccgatc aataaagtga tgtatgacaa
     9781 taagatccac gtgctgaaaa acgcgctcgg gttacatact gccgtttcgc gtgtgcaggg
     9841 cggcaagctg aaagcaaaag cagagatccg cgtcgctacc gtgtttcgca acgcgccgga
     9901 accgtttttg cgcatgatcg tggtgcacga gctggcgcac ctgaaggaga aagagcataa
     9961 caaagcgttt taccagttgt gctgtcatat ggaaccgcag taccaccagc ttgagttcga
    10021 cacccggctg tggctaacgc agttgtcgct tgggcaggac aaaatctgaa aatgatgtca
    10081 ggcgttattt tttcctcctt tagcgcctaa aatcgatctc ctccctttcg ttaaggataa
    10141 cgaccatgct gcgcgcattt gcccgccttc ttcttcgtat ttgtttttct cgccggactc
    10201 ttaaaattgg ctgtttattg ttgcttatag caggagcgac catttttatt gccgatcgcg
    10261 ttatggtcaa cgccagcaag cagctgacct ggagtgatgt caacgccgtt ccggcgcgga
    10321 acgtgggttt attgctgggg gcgaggccgg gtaatcgcta ctttacccga cgtattgata
    10381 gcgccgcaga gttgtaccac gccggaaaag tgaaatggct attggtgagc ggcgataatg
    10441 gacgtaaaaa ttacgatgaa gcgtcaggaa tgcagcaagc cttgatcgct aaaggcgtac
    10501 cggcaaaagc catcttctgc gactatgccg gattctcaac gcttgattcg gtggtgcgtg
    10561 ccaacaaggt gtttagtgaa aaccatatca ccattatctc gcaagaattt cataaccaac
    10621 gcgccatctg gctggcaaaa cagtatggta tcgatgctat cggttttaac gcgccagacc
    10681 tgaatatgaa gcacggttta tatactcaac tgcgggaaaa acttgccaga gtcagcgcgg
    10741 tgattgatgc gaaaatcctc catcgccagc cgaaatatct cgggccgttg gttatgattg
    10801 ggccgtttag tgagcatggc tgtccggcaa aagaataatg cgtatctgcg cacgtcgaag
    10861 ataaaaaagg cgtgctacag tgacgacaga atccctttat ggagtatcca cgcgttatga
    10921 tacgtttcgc tgtgattggt acgaactgga tcactcgcca gttcgtcgag gccgcccatg
    10981 agagcggtaa atacaagtta accgccgtct attcccgcag ccttgaacag gcccagcact
    11041 tcgccaatga tttttctgtc gagcatctgt ttacgtcgct ggaagcgatg gcagaaagcg
    11101 atgccattga cgcggtgtat attgccagcc caaattccct gcacttttcc cagacacaac
    11161 ttttccttag tcacaaaatt catgtgattt gcgagaagcc actggcgtcg aatcaggcgg
    11221 aagtggatgc cgccattgcc tgtgcgcggg aaaatcaggt ggtgctgttt gaggcattta
    11281 aaacggccta cctgccgaac tttcatctgt tacgccaggc gctgccgaaa gtcggcaaac
    11341 tgcgtaaagt ctttttcaac tattgccagt attcctcgcg gtatcagcgt tacctggacg
    11401 gagagaatcc caacaccttc aatccggcat tctccaacgg ttcgattatg gatatcggct
    11461 tttactgcct ggcgtcggcg gtggcattat tcggtgaacc gaaaagcgtg caggcaaccg
    11521 ccagtttgct ggcaagcggt gtggatgccc acggcgtggt ggtgatggat tacggtgatt
    11581 tcagcgttac gttgcagcac tcaaaagtga gcgattctgt cctggcaagc gagattcagg
    11641 gcgaagcggg atcgctggtg attgaaaaac tctctgaatg ccagaaagtc tgctttgtac
    11701 cgcgtggcag ccagatgcag gatctcaccc agccacagca tattaatacc atgctctacg
    11761 aagcagagct gttcgctacc ctggtggatg agcatctggt gaatcatcca gggctggcgg
    11821 tcagtcgtat caccgccaaa ctgctgactg agatccgccg ccagactggg gtgatttttc
    11881 cggcagataa cgtaaaacta taattgccaa agtaaaacag tgtaaaaggt atgtaacaga
    11941 ccattgactg actgaatggt ctgtcatact ttgttacctg caaaggggag taacttcatt
    12001 gtcggtcgat cgtcattacg gtgtgtgaaa aaccacatcc ggtcaccggg caacccgaaa
    12061 ggaatacgca gacgtattcc ttttttgttg taagtgagac cttgccggaa ggcgaggtct
    12121 atgcataaaa agcagcggct gacgtcttcc gacgttggcc gtttttttat gtgtaaggaa
    12181 cttctatgaa tactgtcggc acgccgttgc tatggggcgg attcgctgtt gttgtcacca
    12241 ttatgctggc tatcgacctg ttgttgcagg ggcgtcgtgg ggcacatgcc atgaccatga
    12301 aacaggctgc ggcctggtcg ctggtctggg tgacgctgtc gttactgttt aacgccgctt
    12361 tctggtggta tctggtgcaa accgaaggtc gcgccgttgc cgatccacag gcactggcct
    12421 ttctcacggg ttatctgatt gagaaatcgc tggcggtcga taacgtcttt gtctggctga
    12481 tgttgttcag ctatttctct gttccggcgg cattacaacg ccgcgtgctg gtgtatggcg
    12541 tactcggggc gattgttctg cgtaccatca tgatcttcac tggtagctgg ctgatttcac
    12601 agttcgactg gatcttgtat atcttcggtg ccttcctgct gtttaccggc gtgaagatgg
    12661 cgctcgccca tgaagatgaa tcaggcattg gcgacaagcc gctggtgcgc tggctgcgcg
    12721 gtcatttgcg catgaccgac accatcgaca acgagcattt ctttgtgcgt aagaatggtt
    12781 tgttgtatgc cacgccgctg atgctggtgc tgattctggt ggagttgagc gacgtgattt
    12841 tcgccgtgga tagtattccg gctatcttcg ccgtgaccac cgatccgttt attgtgctga
    12901 cctcaaacct gtttgcgatc ctcggcctgc gtgcgatgta tttcctgctg gcaggtgtgg
    12961 cagagcgttt ctcgatgctc aaatatggtc tggcggtgat tctggtgttt atcggtatca
    13021 agatgctgat tgtcgacttc taccatattc cgatcgccgt ctcgctgggc gtggtgtttg
    13081 gcattctggt gatgacgttt attatcaacg cctgggtgaa ttatcggcat gataagcagc
    13141 gggttggatg atttttaatc tgcctaagcc gtgtaccctg tcatccacat gagcaccgtt
    13201 ttctccctct ccccaaaagg ggcgagggga ctgaccgagt acttttttga cgttgtcatc
    13261 agtctggaag ccgcacgttg ggctttattt tcatgtcaaa caaatgtaac cattaagttg
    13321 cgaaatatga cttctcctta aaatccagca tatttcgctt cccgaagctg taactttcct
    13381 tatactcgac cttgcaaaca ctttgttaca tcctgaaaga tgcgtcgaca gaacgcacca
    13441 gggatgtgcg acaacacaat gaaaggatcg aaaaatgact acgcaacatt caccggggct
    13501 attccggcgt ctggctcatg gcagcctggt aaaacaaatc ctggtcggcc ttatactggg
    13561 gattcttctg gcatggatct caaaacccgc ggcggaagct gttggtctgt taggtacttt
    13621 gttcgtcggc gcactgaaag ccgttgcccc catcctggtg ttgatgctgg taatggcatc
    13681 tattgctaac caccagcacg ggcagaaaac caatatccgc cctattttgt tcctctatct
    13741 gctgggcacc ttctctgctg ctctggccgc agtaatcttc agttttgcct tcccttctac
    13801 cctgcacttg tccagtagcg cgggtgatat ttcgccgccg tcaggcattg tagaagtgat
    13861 gcgcgggctg gttatgagca tggtttccaa ccccattgat gcgctgctga aaggtaacta
    13921 catcgggatc ctggtgtggg caattggcct cggtttcgca ctgcgtcacg gtaacgaaac
    13981 caccaaaaac ctggtcaacg atatgtcgaa tgccgtcacc tttatggtga aactggtgat
    14041 tcgcttcgca ccgatcggta tttttggtct ggtttcttct accctggcaa ccaccggttt
    14101 ctccacgctg tggggctacg cgcaactgct ggtcgtgttg gttggctgta tgttactggt
    14161 ggcgctggtg gttaacccct tgctggtgtg gtggaaaatt cgtcgtaacc cgttcccgct
    14221 ggtgctgctg tgcctgcgcg aaagcggcgt gtatgccttc ttcacccgca gctctgcggc
    14281 gaacattccg gtgaatatgg cgctgtgtga aaagctgaat ctggatcgcg atacctattc
    14341 cgtttctatt ccgctgggag ccaccatcaa tatggcgggc gcagcaatca ccattaccgt
    14401 gttgacgctg gctgcagtta atacgctggg tattccggtt gatctgccca cagcgctgct
    14461 gttgagcgta gtggcttctc tgtgtgcctg tggcgcatcc ggcgtggcgg ggggatctct
    14521 gttgctgatc ccactggcct gtaatatgtt cggtatttcg aacgatatcg ccatgcaggt
    14581 ggttgctgtc ggctttatta tcggcgtatt gcaggactcc tgtgaaaccg cgctgaactc
    14641 gtcaactgac gtgctgttca ctgcggcagc ttgccaggcg gaagacgatc gtctggcaaa
    14701 tagcgccctg cgtaactaat acttagcccc tttcgtctac ggcggaaggg gttttctcca
    14761 ctttaaacgg atcaattccc cttctctgca tacgccagaa acgaatgata ttcaggccat
    14821 tcatcagcag aaaactgcct tcaatcatcg tgccgcctat cgaccccgcc cagaagttgt
    14881 gaatcaccca gcaacacgtt gaaaaccaca ttacgcagcg catggtcagc cctttacagc
    14941 ggaacagcgc ccaggtactg acaatcgtgc cgattaccgg caatagttca acaggatgat
    15001 ggaacttcgc gaggccaatt ccgccagtca gcacaataaa aatcgccatt acccataagc
    15061 tgcgcgtgcg taaggtaatc aatgtacgaa tggcattaag gatggcactg gcaccggcag
    15121 gataggtgcc cagaagaaaa aaatgtacgc caataacggc gctatagacc gaaagttgct
    15181 ttttgaagcg acgttcgtca cgattgaaaa atgttgtgat accaatcaga aaggcgatga
    15241 cacccacgcc ctgggccagc caatacgcgg tcatgataaa tccttagcag gtatggaaaa
    15301 gcaaacggcg cttcacatta tgaaacgccg ttttttatta acaactcatt tcgactttat
    15361 agcgttacgc cgcttttaaa gatcgccagt tcgcggaagt cgttacgctc gttacaggtt
    15421 tgcttaccgt tggcaaactc aacgatggtg tcgataaatt cttccagcaa ctgcggcatc
    15481 gctttaccgt ggatcagctg acccgcgtca aagtcgatcc agtgtttttt cttcgccgcc
    15541 agttcactgt tggtggcgat tttcaccgtc ggcacaaatc caccatacgg cgtgccacga
    15601 ccagtactga acagcaccat atggcagccc gcacccgcca gggcgctggt cgctacggca
    15661 tcgttacccg gcgcacttaa caagttcagc cccggcgttt tcagacgctc gccgtaacgc
    15721 agcacgtcaa ccacgacact ggaacccgct ttctgggtac agccaagcga tttatcttcc
    15781 agcgtggtga taccgcctgc tttgttcccc ggtgatgggt tttcgtagat cggctggtca
    15841 tgggcaataa agtactgctt gaagtcattg accatggtga ccagtttttc aaacgttgct
    15901 tcgtcgcggc aatggtccat cagcaactgc tctgcgccaa acatctccgg cacttcggtc
    15961 agtacggtag taccgccgtt agcaatcacg tagtcagaga aacgccccag catcgggtta
    16021 gcagtaatac cagaaagacc gtcagaacca ccgcactcca gaccaaactt cagttcgctg
    16081 agtttgcccg gctcgcgttt atcgttgcgc atcacgctat acagctgatg caaatgcgcg
    16141 atcccggcct cgatttcatc atcctgctgt tggcagatca tgaaatgaac gcgttcagga
    16201 tcgatatcgc ccaacgtttc acggaatgcg gcaacctggt tattttcgca acccagacca
    16261 atcaccagca ctgcgcccgc gttcgggtgg cgcaccatgt tttgcagcat ggtgcgggta
    16321 ttgatgtgat cgtcgcccag ttgtgagcag ccgtaggtgt ggctgaagag gaacacgccg
    16381 tcggtacctt cagcgttgtt ggtctctttc aggaaacgat tttggatctg ccgcgcgatg
    16441 ccgttgacac agcccacggt tggcaggatc cacagctcat tacgcacccc gacatcaccg
    16501 ttagcgcgac gatagatctg cacttcacga tctgccgctt gcgcaggcag atcctgaaaa
    16561 tcaggttgat agcgatactg atccagatcg ctcagattcg tgcgcgtatt gtgggcgtga
    16621 acgtgttccc ccgccgcaat atccgccaat gcataaccaa tcggcaggcc atatttaatg
    16681 acgttggccc cttttgcgat atccgttaac gcaaatttat gtccacgagc aacatcctgg
    16741 cgcagcgtaa cagtctggtt atcgacactg acttctgtgc cttcagccaa atccgctaaa
    16801 gcaaccgcga cgttatccag cgcatggatc ttgatgtatt gcatatcaac cccagacctt
    16861 agttcagttc aatggcgaag tagtcacgcg cattgttaaa gcaaatattt ttcaccatct
    16921 cgcccagcag gttgatgtcc gccggtgctt cgcctgcttc cacccagcga ccgatcatct
    16981 ggcacagaat gcggcggaag tattcgtgac gggtgtatga caggaagcta cggctgtcag
    17041 tcagcatgcc gacaaagcgg ctcagcagac cgagctgcgc cagttgggtc atctgacgtt
    17101 ccataccgtc tttctggtcg ttaaaccacc agccggaacc gaactgcatt ttgcccggca
    17161 taccttcgcc ctggaagtta ccgatcatgg tgcccagcac ttcgttatcg cgcgggttca
    17221 ggcagtagag aatggttttc ggcagcaggt tttcttcgtt ctgcttgctc agcagcttag
    17281 atagctcttc cgccatcgga cggtcgttga tggagtcaaa gccgacatcc ggccccagca
    17341 gtttgaactg acgcaggttg ttattacgca gtgcgccaat gtggtactgc tgtacccatc
    17401 cgcgacgtgc atattcggca ccaaggaaca ccagcaccgc agttttgaac tgtgctactt
    17461 cgtgctcgct cagggtttcg cctgccagac ggcgcgcgag gatgctgtcc agttccgctt
    17521 cgttcgcttc agcaaacatc accacatcca gcgcgtggtc agacacttta cagccgtgag
    17581 cggcgaagtg atccagacgt ttagtcaggg cagtttgcag gtcagcaaag cggcgaatgt
    17641 cggtatcaga aacttcgccc agcttcgcca tgtagtcgtt aaaggtcgcc tgttcaatgt
    17701 taaaggcttt gtccggacgc cagctcggca gcactttaat ggtgaaagag ccgtctttgg
    17761 cgatctctgc gtgatgctcc agagaatcga tcgggtcatc ggtggtgccg accattttca
    17821 cgttcatctg ctgcatgatg ccgcgcgcgg agaagttatc ctgcgccagc agttcgttac
    17881 attcgttcca gatttcatcg gcagttgacg gagaaagcaa tttgccagtg ataccaaacg
    17941 gacgacgcag ttcgaggtgc gtccagtggt ataacgggtt gccgatagtg tgcggaacag
    18001 tcgccgccca ggcgtcaaat ttttcacggt cagacgcatc accggtgcac agacgctcgg
    18061 ccacaccgtt ggtacgcata gcgcgccatt tgtagtgatc gcccttcaac cagatgtcat
    18121 acaggttttt aaaacgatag tcttccgcaa tctgctgcgg cggcaaatgg caatggtaat
    18181 cgaaaatcgg ctggtctttt gcgtagtcgt gatacagacg gcgggcaaat tcagtatcta
    18241 acaggaaatc ttcagtcata aacggagtca ttttcgtctt cctctcaacg agtgagctag
    18301 cttgcttatg gtgcgatgct gacaaagtta tcacaccaat ttccagagtc cgaagatatt
    18361 ttcgtgagtt agatcaataa acgtagttaa aaaaattact ctcaaagtgg taaatctcgc
    18421 tgcaggccgc gccagtactg gcctcgtcgt cgtcaggtaa tgtccctaca aatattcgca
    18481 catttgtgat ggctctcacc ttttaaagtt gtatgacaag ttatctttct gccgtcgcaa
    18541 atcataagtc gacggaatgc aaattgccga ttcattcatt tgttagatga atcgggttaa
    18601 ccggtacgga agccgaatta gcacgaaact ttcatggcaa cgttcggggc gtgccggttt
    18661 tttttcggtt acccggtcgt aactaacatc ttcagcctct ggcgggatga tggccgcgct
    18721 tcctgcggat ataacaaaac gatgaggttt tacatgcgta aaattaaagg gttacgttgg
    18781 tatatgatcg cactggtgac gctcggcacc gtgcttggtt acctgacgcg taacactgtg
    18841 gcggcagctg cgccaactct gatggaagag ttaaacatct ccacccaaca gtattcctat
    18901 atcatcgcag cgtattctgc tgcttatacg gtcatgcaac cggtagcggg ttatgtactg
    18961 gatgtgctgg gtacgaaaat cggttatgca atgtttgctg tactgtgggc cgtgttctgt
    19021 ggtgcaaccg cgctggcagg tagttggggt ggcctggcgg ttgctcgtgg tgcggtcggt
    19081 gccgcggaag ccgcgatgat tccggcgggt ctgaaagcca gctccgaatg gttcccggca
    19141 aaagagcgtt ccatcgcggt aggttacttt aacgtaggtt cttcgattgg tgcgatgatt
    19201 gcgccgccgc tggtggtatg ggcaatcgtg atgcacagct ggcagatggc atttatcatc
    19261 tccggtgcgt tgagctttat ctgggcgatg gcatggctga ttttctacaa gcatccgcgc
    19321 gaccagaaac atctgaccga tgaagaacgc gactacatta ttaatggtca ggaagcccag
    19381 caccaggttg acacagcgaa gaaaatgtcc gttggtcaga tcctgcgtaa ccgtcagttc
    19441 tggggtatcg cgctgccgcg cttcctggca gaaccggcat ggggtacttt taacgcgtgg
    19501 atcccgctgt tcatgtttaa agtttacggc tttaacctga aagagatcgc gatgtttgcc
    19561 tggatgccga tgctgtttgc tgacctcggt tgtatcctcg gtggttacct gccaccactg
    19621 ttccagcgtt ggtttggtgt gaacctgatc gtttcccgta agatggtcgt aacgctgggt
    19681 gcggtgctga tgattggccc gggtatgatc ggtctgttca ccaacccgta tgtcgcgatt
    19741 atgctgctgt gtatcggtgg ttttgctcac caggcactgt ctggcgcact gattacgctc
    19801 tcgtctgacg tgttcggtcg taacgaagtg gcgacggcga acggtttaac cgggatgtcc
    19861 gcatggctgg caagtacgct gtttgcactg gtagtcggtg cactggctga caccatcggc
    19921 ttcagcccgc tgttcgcagt tctggcagtg ttcgacctgt taggtgcgct ggttatctgg
    19981 accgtgttgc agaacaaacc agcgattgag gtggcgcagg aatcacataa cgatcccgca
    20041 ccgcaacatt aaggcttcgt gtaagtttta gtttcccttc aaagccgcct tctcaggcgg
    20101 ctttttcatc actgcgagta gagctaaact cgcaaaagtg gtataacaaa tatagtctgc
    20161 cgtatcatgc ctggagcgca tatggaaatc actgaaccac gccgtttgta tcaacaactt
    20221 gccgctgatc tgaaagagcg catcgaacag ggcgtctatc tggtgggtga taaactgcct
    20281 gccgaacgct ttattgccga tgaaaaaaac gtcagccgca ccgtggttcg tgaagccatc
    20341 atcatgctgg aagttgaagg ctatgtcgaa gtgcgtaaag gttcaggtat tcatgtggtt
    20401 tccaaccagc cgcgccatca gcaggcggct gacaataata tggaattcgc caattacggt
    20461 ccgtttgagt tgctccaggc tcgccagctc atcgaaagta atattgccga gttcgcggca
    20521 actcaggtca caaaacagga catcatgaaa ttgatggcca ttcaggagca ggcacgcggc
    20581 gaacaatgct tccgtgattc cgagtgggat ttgcagttcc atattcaggt agccctggcg
    20641 acgcagaact ccgccctggc ggctatcgtt gaaaaaatgt ggacccagcg tagtcataac
    20701 ccgtactgga aaaaactgca cgaacacatt gatgcccgta ccgtcgataa ctggtgtgat
    20761 gaccacgatc aaatcctcaa ggcgctgatt cgcaaagatc ctcatgctgc taagctggca
    20821 atgtggcagc acctggaaaa caccaagatc atgttattta acgaaaccag cgacgatttc
    20881 gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tggtgcatct cgatacagcc
    20941 accagcggca gtaaatgaaa ttcctgtccg acaggcgcta cagtgcgcct gtttgtctcg
    21001 tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgcctc cctcccctgc
    21061 attcagcaaa atcagactcc atggacatgt aattttgata acgaacaacg ttgacctttg
    21121 ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc acgatttaac
    21181 agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa gacagcaata
    21241 acattgcggt agtgcatcat ttaaaaccag cgtggcgtta accgattcac caggaataat
    21301 gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg
    21361 ccaatccgtc gatgattggc atgttgtatt ttgtcttgtt tgtcattttg ttccttgaaa
    21421 acggcttgct tccggcggcc tttttaccgg gcgacagttt actggtactg gtcggcgtat
    21481 tgattgcgaa aggcgcgatg ggctatccgc aaacgattct gctgctgacc gttgccgcca
    21541 gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc cgcaccgtac
    21601 aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat ctttttcata
    21661 aacacggttt atcggcgctg ttaattggtc gctttattgc gtttgtcaga acactgctgc
    21721 cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc aactggatga
    21781 gtggtctgct gtgggtgttg atcctgacaa ctctgggtta catgctcggc aaaacaccgg
    21841 tatttttaaa gttcgaggac cagctgatgt catgcctgat gctgctcccg gtggtgctgc
    21901 tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat ggaaatcggg
    21961 ggtaagggat gcaaatacct cgcatgtcac ttcgccagct agcctggtcc ggcgctgttt
    22021 tacttctggt cagcacacta ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc
    22081 tggcgattcg tgccgttcat caagggacaa cgatgccaga cggtttttca atctggcatc
    22141 accttgacgc tcacggcatt cctttcaaaa gtatcacccc caaaaacgac actctgttaa
    22201 ttacctttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc
    22261 cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag tggctgaccc
    22321 ggttgcggga taactctcat cgcttcggat aacttcctgt tatccgaaac agatcactca
    22381 ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac acgaccgctt
    22441 cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag gttcaagaat
    22501 gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta gttatgccac
    22561 taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg ccgaaaaaca
    22621 ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc gggccaactg
    22681 ttcagatagc caactgcgtg ccgatcatca gaagaaaatc gcaaagcaga aagatgaggt
    22741 ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgcag ataagattgc
    22801 caaacgcgaa cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga
    22861 ctactaactc acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca
    22921 ctacggaaca tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta
    22981 gctcatctgg cgagaagtca aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg
    23041 cactgaaaca gagccgttat cgcctgggtg aaaccggtga tgccattgcc aaacaaaccc
    23101 gtgtcgcggc ggcgcgtgcc gatgagtatg tgcgtgaaaa tccgtggacg ggcgtgggca
    23161 ttggcgcagc aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga
    23221 cactcatcac gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc
    23281 catcatggtt gaaatggtgg agacacgtct gcggctggcg gtggtggagc tggaagagga
    23341 aaaagcgaat ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt
    23401 tggtcttatg agcctgatgg tgctaattat ctgggcggtt gatccgcaat atcgcctgaa
    23461 tgcgatgatt gccaccaccg tggtgttgct gctactggca ctgattggcg gcatctggac
    23521 gttacgtaaa tcgcgtaagt ctacgttgct gcgccacacg cgccatgagc tggcaaacga
    23581 tcggcagctg ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg
    23641 cgcaactgct tagccagatc cagcaacaac ggctggatct ttccgccact cgtcgtgaat
    23701 ggctggaggc aacaggcgct tacgatcgtc gctggaatat gctgttgagt ctgcgctcct
    23761 gggcgctggt tggcagtagc gtgatggcta tctggacgat tcgccatcct aatatgctgg
    23821 tccgctgggc cagacgcggt tttggtgtat ggagcgcctg gcgtctggtg aaaacaaccc
    23881 tcaagcaaca acagcttcgc ggttaacttc ctctctggcc ggagaaattc cggccttatt
    23941 cctcaaattt tttgaaaata tttgacagtt ttccttgcta acaatcacgc ttcgccacgt
    24001 ttatgattct ctccatcgac agcaacgacg ctaatagcgc gcccttgcac aaaaaaacaa
    24061 tcagcagcct gagcggcgca gtggagagta tgatgaaaaa attagaagat gttggtgtac
    24121 tggtagcgcg tattttaatg ccgattctgt ttattaccgc aggctgggga aaaattactg
    24181 gctacgcggg tacccaacaa tatatggaag caatgggcgt ccccggcttt atgctgccgc
    24241 tggtgattct gcttgagttt ggtggtggtc tggcaatcct gttcggtttc ctgacacgta
    24301 ccacagccct gtttactgcg ggctttacgc tgctgacggc atttttattt cacagcaatt
    24361 ttgcagaagg catgaactcg ctgatgttta tgaaaaacct gactatttct ggcggattcc
    24421 tgctgctggc aattaccggt ccaggcgcat tcagcatcga ccgcctgctg aataaaaagt
    24481 ggtaagcacg cgctatactt aacgataaaa aacgaggggg aagctcctcg tttttgctat
    24541 tggaggatag aaaaatgggt caactgattg acggcgtctg gcatgacacc tggtacgata
    24601 ccaaatccac cggcggtaaa tttcaacgtt cagcttccgc atttcgtaac tggctcactg
    24661 ccgatggcgc gccaggcccc actggcaaag gcggttttgc cgcagagaaa gatcgttatc
    24721 atctctatgt ttcactcgcc tgcccgtggg cacaccgcac gctgatcatg cgtaagctca
    24781 aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg gaaaacggct
    24841 ggacctttga tgacagtttt ccgggagcaa gcggcgacac gctctatcaa catgaatttc
    24901 tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact gttcccgtgc
    24961 tgtgggacaa aaaaaaccac accatcgtca gcaacgaatc agcagaaatc atacgcatgt
    25021 tcaataccgc gtttgatgcg ctgggtgcga aagctggaga ttactaccca ccagccctgc
    25081 aaccgaaaat tgacgaactt aacggctgga tttacgacac cattaacaat ggcgtgtata
    25141 aagccggttt tgccaccagc cagcaagcct acgacgaggc ggtggcgaaa gtgtttgaat
    25201 cgctggcgcg actggaacag attttaggtc agcaccgtta cctgaccggc aaccagctaa
    25261 ccgaagccga tattcgcctg tggaccacgc tggtgcgttt tgatccagtg tatgtgaccc
    25321 acttcaagtg tgataagcac cgcatcagcg attacctgaa tctgtatggc ttcctgcgcg
    25381 atatctacca gatgccggga attgccgaaa cagtcaattt cgatcacatc cgtaatcatt
    25441 acttccgcag ccataagacc atcaacccta cggggattat ttcaattggt ccgtggcagg
    25501 atctcgatga accgcatgga cgagatgttc gcttcggtta aaaaaaggca ccttacgagg
    25561 tgcctctgat aattagctaa gtacgtgttg ttcatgtcgg atgcggcgta aacgccttat
    25621 caaacctaca aaacatttat attcaatatg ttgcaatgac cgcgcaggcc agataagcgt
    25681 agcgcatctg gcaattttgc gtttgccctt atccttcaca gcgccttatt aattcatcgc
    25741 gataatattt actgccattt cattcacgtc tattcttaat ttgctgcttt aaaaacaagt
    25801 gattgagcaa aattgaggca aaaatggact ggtatctgaa agtactaaaa aattatgtcg
    25861 gtttccgggg tcgtgcgcgg cgcaaagagt actggatgtt tattctggtc aacatcatct
    25921 ttacgttcgt gctggggcta ctggataaaa tgttaggctg gcaacgtgcc ggtggcgaag
    25981 gcatcctgac gacaatctac ggtattctgg tgtttttacc atggtgggca gttcagttcc
    26041 gccgcctgca cgacaccgac cgctcggcgt ggtgggcact gctattctta atcccgttta
    26101 tcggctggct tattatcatc gtctttaact gccaggcggg tacgccaggc gaaaaccgct
    26161 ttggaccaga tccgaaactg gaacaagaat aataattaag caccttgtaa aaggtgctta
    26221 atcgattatt ttccgctaaa aagtttggga atttcccgca gacaccagga tttcgcctcc
    26281 cccatactgt cacgacgcca ggccataata atatcgatct cgctggtcga ttccgggctg
    26341 acgacacgca accgcccttc cgcaatatct ttttcgacca tcggatacgg catcgtcgcc
    26401 acgccaagcc ccgccagtaa tgcctgacgt ttatcttcaa tcgtgctcac cgttaagcgc
    26461 ggctgtttgt ccagcaactg tacggtcaac accgggcgct cacgagcggt atccgccacc
    26521 gcaattccac gatatttcac gcgcgtcact tcagataacg gttccggctc ctgatgaatc
    26581 gggtgatccg gcgcggcaac atagacgttc attaaggtat agagtttgcg cgagttgatc
    26641 tccgacgagg aacgaaaatg catatccggc gcgataacga tatccgcccg cccctgctcc
    26701 agccgttccc acgcccccgc cagcacttcg gtgataattg ccagttgggt attggctttt
    26761 gccgccagtt tgtcgattaa cgggaaaaag gcaggtgtcg gtaccagcgc ctcggtcaca
    26821 atggtgagat gcgtttccca accgcgcgcg agggcttccg catcggtagt cagtttatct
    26881 gcggcttcca gcaaaacgcg ccctcgctcc agcaacatcc tcccgacatt ggtgaatttg
    26941 gtgcgatggc ccgagcggtc aaacagcacc acatccagct cttcttccag tttttgcatg
    27001 gtgtagctaa gtgcggaagg cacgcgtccc agctcatccg ccgccgccgc aaaactgccc
    27061 cggcgatcga tcgcatccat aacccgtagt gcttccagcg ttaatgccct ttctttggcc
    27121 atttcgttct cattcaggaa atttgaacat accgggcaga atatctggct aacaatgtag
    27181 cgtccagccc cttaccataa aaggaagtaa agagaggtca agaattatga ttactacccg
    27241 aactgccagg cagtgtggac aagcagacta cggatggttg caggcccggt atactttttc
    27301 ctttggacac tacttcgacc cgaaattgtt aggctatgcc tctctgcgcg tgcttaacca
    27361 ggaagtgctg gccccaggtg ccgcctttca gccacgaacc tatcccaaag tcgatatttt
    27421 aaatgtgatt ctggatggag aagctgagta tcgcgacagc gaaggcaatc atgttcaggc
    27481 cagcactggt gaggtgttgc tgctctctac ccagccgggt gtcagctata gcgaacacaa
    27541 tctcagcaaa gacaaaccgt taacgcgaat gcagctttgg ctggatgcct gcccgcagcg
    27601 agagaatccg ctgattcaaa agctggcgct taatatgagc aagcagcaat taatcgcctc
    27661 gccagaaggt gggatgggaa gcctgcaatt gcgccagcaa gtgtggctgc accatatcgt
    27721 gctcgacaaa ggcgaaagtg cgaatttcca gttgcatggg ccacgcgcgt atttgcaatc
    27781 gattcacggg aaatttcatg cgcttacgca tcatgaagag aaagcggcgc taacctgcgg
    27841 tgatggggcg tttattcgtg acgaggctaa cattacgctg gttgccgatt ccccactgcg
    27901 cgctttgctg atagatttgc ctgtctagtt gtttttacag ggagatgatg atgagtaaaa
    27961 aatcggccaa aaagcgccag ccagtgaagc ccgtggtggc gaaagaacct gctcgtaccg
    28021 ccaaaaattt tggctatgaa gagatgttga gcgaactgga agccatcgtc gcggatgctg
    28081 aaacgcgttt agccgaggat gaagctaccg cgtaaagaca ggttgccgga tgcggcgcaa
    28141 acgccttatc cggcacaata cccaaaaccg ggcgtttatc tggccttgct cgccataatc
    28201 tcgataatct gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca
    28261 atcgactgct caacatcatg cgccacaatc ccttcattgc cggtcacggc ggtatcatcc
    28321 agcgccatta acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag
    28381 ctgttcgatg caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc
    28441 atcgagatgg tttcataacg cccatccacc agccatgcca tcccggcagc ggcccccatt
    28501 gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatc
    28561 gcgctcaaat gcgaaagcat cagcgcacgc gccagccgtt catcatccgc tccgaagtgt
    28621 tcagctacca ccaccacggg cattgttgcg gtgatcccct ggttacccga gccggagtta
    28681 ctcattgccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc
    28741 acaatggatg aagagagatc tttcgccagc aaaccgcgcg cgcactgttt ttccagcgtc
    28801 gcaccaatat gcagccccca gttaccgctc aaaccttcct gcgataacgc acaatttaac
    28861 ttcgcagaat cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg
    28921 atctcagcca gcgtcgtcct ggaaagcacc gtcagcggcg actcttgctc gccctctgcc
    28981 acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta
    29041 tgcccgccga cgatggtgac acacgcccat ttctcaccgt tccagacttt ggcgcgtgag
    29101 aagaggattt catcgcaagg ttcctggatc ttaactgaga ctttccccgc cgccagcagt
    29161 gctttggcat cggaaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgtta
    29221 gcatttccac ctaacgctcc cagcgccgcc gcaattggca gccccaccat tcctgtaccg
    29281 ggaacggtga cacccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt
    29341 tcaaccggac cttccagttc agccgcagca accgccgccg ccagcgccag tgaaatcggt
    29401 tcagtacacc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat
    29461 aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa
    29521 aatgccagga acggagaaac acaaagcaac aaaccagtga cgataatcag gtacaaagac
    29581 atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac
    29641 cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg ggcgttcagt
    29701 acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacgagattt
    29761 tcgttaatct tctcggcagg tatcttgcga cgcaggatgt tcattacgat cccttgcgtt
    29821 gcttcgcgaa agcctaaata gacgccaaag aatgcagtca ttacagcaaa gatattgaga
    29881 atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc
    29941 gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc
    30001 gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt
    30061 gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc
    30121 accatcgggc ttaacgtctg gataaataga atcgacgtca gggtaaacgg cagcgtaata
    30181 atggcgtttt tcatcagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac
    30241 attcccacca tcgacacgcc cagcgccgcg accaccagca gcttggtcag caccatgccg
    30301 gtcgaaattt tgaataacaa tttctcgccg cgtgaggaga tcgccaccag aatgcaaatc
    30361 agtaccagac cataaaaggg actgtctga
//
LOCUS       DAOFSV010000045        30046 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.045, whole genome shotgun sequence.
ACCESSION   DAOFSV010000045 DAOFSV010000000
VERSION     DAOFSV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.045"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            53..1249
                     /gene="ispC"
                     /locus_tag="QBH27_002138"
     CDS             53..1249
                     /gene="ispC"
                     /locus_tag="QBH27_002138"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDQ7014448.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALKDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            1438..2196
                     /gene="ispU"
                     /locus_tag="QBH27_002139"
                     /gene_synonym="uppS"
     CDS             1438..2196
                     /gene="ispU"
                     /locus_tag="QBH27_002139"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDQ7014449.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            2209..3066
                     /gene="cdsA"
                     /locus_tag="QBH27_002140"
     CDS             2209..3066
                     /gene="cdsA"
                     /locus_tag="QBH27_002140"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDQ7014450.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            3078..4430
                     /gene="rseP"
                     /locus_tag="QBH27_002141"
     CDS             3078..4430
                     /gene="rseP"
                     /locus_tag="QBH27_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDQ7014451.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            4460..6892
                     /gene="bamA"
                     /locus_tag="QBH27_002142"
     CDS             4460..6892
                     /gene="bamA"
                     /locus_tag="QBH27_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDQ7014452.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            7014..7499
                     /gene="skp"
                     /locus_tag="QBH27_002143"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             7014..7499
                     /gene="skp"
                     /locus_tag="QBH27_002143"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDQ7014453.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            7503..8528
                     /gene="lpxD"
                     /locus_tag="QBH27_002144"
     CDS             7503..8528
                     /gene="lpxD"
                     /locus_tag="QBH27_002144"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDQ7014454.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            8633..9088
                     /gene="fabZ"
                     /locus_tag="QBH27_002145"
     CDS             8633..9088
                     /gene="fabZ"
                     /locus_tag="QBH27_002145"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDQ7014455.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            9092..9880
                     /gene="lpxA"
                     /locus_tag="QBH27_002146"
     CDS             9092..9880
                     /gene="lpxA"
                     /locus_tag="QBH27_002146"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDQ7014456.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            9880..11028
                     /gene="lpxB"
                     /locus_tag="QBH27_002147"
     CDS             9880..11028
                     /gene="lpxB"
                     /locus_tag="QBH27_002147"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDQ7014457.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            11025..11621
                     /gene="rnhB"
                     /locus_tag="QBH27_002148"
     CDS             11025..11621
                     /gene="rnhB"
                     /locus_tag="QBH27_002148"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDQ7014458.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            11658..15140
                     /gene="dnaE"
                     /locus_tag="QBH27_002149"
     CDS             11658..15140
                     /gene="dnaE"
                     /locus_tag="QBH27_002149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDQ7014459.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            15153..16112
                     /gene="accA"
                     /locus_tag="QBH27_002150"
     CDS             15153..16112
                     /gene="accA"
                     /locus_tag="QBH27_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDQ7014460.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            16210..18351
                     /gene="ldcC"
                     /locus_tag="QBH27_002151"
     CDS             16210..18351
                     /gene="ldcC"
                     /locus_tag="QBH27_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDQ7014461.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDSWF
                     FDIWQPPQVDEAECWHVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLQI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            18408..18797
                     /locus_tag="QBH27_002152"
     CDS             18408..18797
                     /locus_tag="QBH27_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ7014462.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            18862..20175
                     /gene="tilS"
                     /locus_tag="QBH27_002153"
     CDS             18862..20175
                     /gene="tilS"
                     /locus_tag="QBH27_002153"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDQ7014463.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(20209..20469)
                     /gene="rof"
                     /locus_tag="QBH27_002154"
     CDS             complement(20209..20469)
                     /gene="rof"
                     /locus_tag="QBH27_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDQ7014464.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(20456..20656)
                     /locus_tag="QBH27_002155"
     CDS             complement(20456..20656)
                     /locus_tag="QBH27_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDQ7014465.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            20822..21367
                     /locus_tag="QBH27_002156"
     CDS             20822..21367
                     /locus_tag="QBH27_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDQ7014466.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSNCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            21364..21786
                     /gene="arfB"
                     /locus_tag="QBH27_002157"
     CDS             21364..21786
                     /gene="arfB"
                     /locus_tag="QBH27_002157"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDQ7014467.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            21800..22510
                     /gene="nlpE"
                     /locus_tag="QBH27_002158"
                     /gene_synonym="cutF"
     CDS             21800..22510
                     /gene="nlpE"
                     /locus_tag="QBH27_002158"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDQ7014468.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNGEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(22666..23490)
                     /locus_tag="QBH27_002159"
     CDS             complement(22666..23490)
                     /locus_tag="QBH27_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDQ7014469.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGNNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSNVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(23543..25261)
                     /gene="proS"
                     /locus_tag="QBH27_002160"
     CDS             complement(23543..25261)
                     /gene="proS"
                     /locus_tag="QBH27_002160"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDQ7014470.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(25372..26079)
                     /gene="tsaA"
                     /locus_tag="QBH27_002161"
     CDS             complement(25372..26079)
                     /gene="tsaA"
                     /locus_tag="QBH27_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDQ7014471.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(26076..26480)
                     /gene="rcsF"
                     /locus_tag="QBH27_002162"
     CDS             complement(26076..26480)
                     /gene="rcsF"
                     /locus_tag="QBH27_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDQ7014472.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(26598..27413)
                     /gene="metQ"
                     /locus_tag="QBH27_002163"
     CDS             complement(26598..27413)
                     /gene="metQ"
                     /locus_tag="QBH27_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDQ7014473.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(27453..28106)
                     /gene="metI"
                     /locus_tag="QBH27_002164"
     CDS             complement(27453..28106)
                     /gene="metI"
                     /locus_tag="QBH27_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDQ7014474.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(28099..29130)
                     /gene="metN"
                     /locus_tag="QBH27_002165"
     CDS             complement(28099..29130)
                     /gene="metN"
                     /locus_tag="QBH27_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDQ7014475.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            29318..29890
                     /gene="gmhB"
                     /locus_tag="QBH27_002166"
     CDS             29318..29890
                     /gene="gmhB"
                     /locus_tag="QBH27_002166"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDQ7014476.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         7187 a         7335 c         8279 g         7245 t
ORIGIN      
        1 ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt tcatgaagca
       61 actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca
      121 taatcccgaa catttccgcg tagttgcgct ggtggccggc aaaaatgtca ctcgcatggt
      181 agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa
      241 acttcttaaa acgatgctac agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca
      301 agccgcttgc gatatggcag cgcttaagga tgttgatcag gtgatggcag ccattgttgg
      361 cgctgctgga ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc
      421 caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa
      481 agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt taccgcaacc
      541 tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact
      601 taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc
      661 cgatcaagcc tgccgtcatc cgaactggtc gatgggacgt aaaatttccg tcgattcggc
      721 taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc
      781 cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctacca
      841 ggacggcagt gttctggcgc agctgggtga accggatatg cgtacgccaa ttgctcacac
      901 catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg cttgattttt gcaaactaag
      961 tgcgttgaca tttgccgcac cggattatga tcgttatcca tgtctgaaac tggcgatgga
     1021 ggcgtttgaa caaggccagg cagcgacgac agcattgaat gccgcaaatg aaatcaccgt
     1081 ggctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatccgt
     1141 actggaaaaa atggatatgc gcgaaccaca atgtgtggat gatgtgttat ctgttgatgc
     1201 gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag gataatccgg
     1261 ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct gcgccatctg
     1321 atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg cttttttttg
     1381 tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataagaa acgcgtgatg
     1441 ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgtcg tcatgttgcg
     1501 atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg tgcctttggg
     1561 cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa caacggtatt
     1621 gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc gcaggaagtc
     1681 agtgcgttaa tggaactgtt tgtgtgggcg ctagatagcg aagtaaaaag tctgcaccga
     1741 cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg tttgcaagaa
     1801 cgtattcgta aatctgaagc gctaacagcc gggaataccg gtctgacgct gaatattgcg
     1861 gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc tgaaaaggtg
     1921 cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca gcatgtctgt
     1981 atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca tcgcattagt
     2041 aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt tctctggccc
     2101 gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt
     2161 ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat
     2221 cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg
     2281 ccggtggggt tcgccattgt aacgctggtg gtctgtatgc tggcagcgtg ggaatgggga
     2341 cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta
     2401 ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca tcaaccgctg
     2461 gttgaaatct cactttgggc ttcgctgggt tggtggattg tcgcgctatt gctggtgctg
     2521 ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcat tatttttggc
     2581 gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg gcactacgac
     2641 gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt atggggcgct
     2701 gactctggcg catatatgtt tggcaaattg tttggtaaac ataaactggc accgaaggtt
     2761 tctccgggta aaacctggca aggctttatc ggtggactcg tgaccgcagc ggtaatctca
     2821 tggggttatg gcatgtgggt gaatctcgac gtcgctcccg tcaccttact catttgctct
     2881 attgtcgcag cgttagcctc agtgctcggc gatctgaccg aaagtatgtt taagcgtgaa
     2941 gcgggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt agatcgtatt
     3001 gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt attcaggacg
     3061 ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc atcgttgcac
     3121 tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg cgttgtggtg
     3181 ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga actgataagc
     3241 tcggcaccga atatgttatc gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg
     3301 agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat aaatctgtcg
     3361 gccaacgagc ggcgattatt gccgcaggtc cagttgcaaa cttcattttt gctatctttg
     3421 cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag
     3481 cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta aaagccgtag
     3541 atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat aaaattggcg
     3601 atgaaagcac caccattacg gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc
     3661 tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct tcgttgggga
     3721 ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca aactcggcgg
     3781 caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa
     3841 cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc ttagcgttag
     3901 aaatcgaaag acaggggagt cccttgtctt tgacattaat cccggagagt aaaccgggta
     3961 atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg cctgatgagt
     4021 ataaagttgt acgccagtat gggccgttca acgctatcgt cgaagccacg gacaaaacgt
     4081 ggcagttgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac
     4141 tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca gcggaactcg
     4201 gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg ataattaacc
     4261 tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc gaaaagatca
     4321 agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc tcgattctgc
     4381 tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga gagttagtta
     4441 ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag
     4501 cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct
     4561 tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag gcgacacggt
     4621 taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca actttgagga
     4681 tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac gtccgaccat
     4741 tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga agcaaaacct
     4801 cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg ccgatatcga
     4861 gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg ttaaagctgt
     4921 cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg aaggtgtgtc
     4981 agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg atgaactgat
     5041 ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata
     5101 ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agttactatc tggatcgcgg
     5161 ttatgcccgt ttcaacatcg attctaccca ggtcagtctg acgccagata aaaaaggtat
     5221 ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag
     5281 cggcaatctt gccgggcact ccgctgaaat tgagcaactg actaagatcg agccgggcga
     5341 gctgtataac ggcaccaaag tgaccaaaat ggaagatgac attaaaaagc ttcttggtcg
     5401 ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac
     5461 cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tatgtgcgta agatccgttt
     5521 tgaaggtaac gatacctcga aagatgctgt cctgcgtcgc gaaatgcgtc agatggaagg
     5581 tgcatggctg gggagcgatc tggtcgatca gggtaaggag cgtctgaacc gtctgggctt
     5641 ctttgaaact gtcgataccg atacccaacg tgttccgggg agcccggatc aggttgatgt
     5701 agtttacaag gtgaaagaac gtaacaccgg tagcttcaac ttcgggattg gttacggtac
     5761 tgaaagtggc gtgagcttcc aggctggtgt gcaacaggat aactggttag gtacgggtta
     5821 tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac
     5881 caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct ataatgactt
     5941 ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta cagacgtgac
     6001 gttgggcttc ccgatcaacg aatataactc gctgcgtgca ggtctgggtt atgtacataa
     6061 ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact ctatgggtga
     6121 acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca cgttcaacta
     6181 tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa
     6241 cctgaccggt aaagtgacca ttccgggatc ggataacgaa tactacaaag tgacgttaga
     6301 cacggcgact tatgtgccga ttgatgacga tcacaaatgg gttgttctgg gtcgtacccg
     6361 ttggggttat ggtgatggtt taggcggcaa agagatgccg ttctatgaga acttctatgc
     6421 cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga aagcagttta
     6481 cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg cgactcagga
     6541 cggcgcgaaa gatctgtgta aatcggatga tgctgtaggc ggtaacgcca tggcggttgc
     6601 cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta actcggttcg
     6661 tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt ccagccaata
     6721 ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg gtatcgcatt
     6781 acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt tcaaaaagta
     6841 cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt aagtgttctc
     6901 cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata agatcgccgg
     6961 gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt attgtgaaaa
     7021 agtggttatt agctgcaggt ctcggtttag cactggcaac ttctgctcag gcggctgaca
     7081 aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa accggtgttt
     7141 ctaacacgct ggaaaacgag ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg
     7201 atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat cgcactaagc
     7261 tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag gcttttgagc
     7321 aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt atccagactg
     7381 ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca aacgccgttg
     7441 cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag gttaaataag
     7501 taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa ctacacggtg
     7561 atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta
     7621 cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg tccgcggttg
     7681 tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg aagaatccct
     7741 acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc gcgcagaaca
     7801 ttgcacccag tgcggtgatt gacgcgacgg cgaagctggg taacaacgta tcgattggcg
     7861 ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt
     7921 gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg aacgtaacca
     7981 tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg
     8041 cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca cagattggtc
     8101 gggtaattat tggcgatcgc gtggagatcg gtgcctgcac tactattgat cgtggcgcgc
     8161 tggatgacac tgttattggc aatggtgtga tcattgataa ccagtgccag attgcacata
     8221 acgtcgtgat tggcgacaat acggcagttg ccggtggcgt cattatggcg ggcagcctga
     8281 aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat atggaaatat
     8341 gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact gaaccaggcg
     8401 tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg
     8461 tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac
     8521 aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt cttacgattg
     8581 cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta tcttgactac
     8641 taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc gtttcccgtt
     8701 cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa
     8761 tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga ttttcccggg
     8821 tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta aaagcgtagg
     8881 aaaactggaa ccgggtgagc tgtactactt cgccggtatt gacgaagcgc gctttaagcg
     8941 cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg
     9001 cctgacccgt tttaaagggg ttgctctggt cgacggtaaa gtagtttgcg aagcaacgat
     9061 gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct ttgtgcatcc
     9121 aaccgccatt gtggaagagg gcgcgtcgat tggcgcgaac gctcacattg gtcctttttg
     9181 tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc acgttgtcgt
     9241 gaatggtcat actaaaattg gccgcgataa tgagatttat cagttcgcct ccatcggcga
     9301 agttaaccag gatctgaaat atgctggcga accgacccgc gtggaaatcg gcgatcgtaa
     9361 ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg gattgacgaa
     9421 ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg
     9481 taaccgctgc atcctcgcca acaacgcaac gctggcgggt cacgtatcgg ttgacgactt
     9541 cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg cgcatgtgat
     9601 ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgtcattg cgcagggtaa
     9661 ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat tcagccgtga
     9721 ggcgattacc gctatccgca atgcgtataa gctgatttat cgtagcggta agacgctcga
     9781 tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga aagcctttac
     9841 cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgaaca gcgtccatta
     9901 acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg tttaatccgc
     9961 gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc acgaatgcag
    10021 gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cagtgatggg cattgttgaa
    10081 gtgctcggtc gtctgcgtcg cttactacat attcgtgccg atctgacaaa gcgttttggc
    10141 gaactgaagc cagatgtttt tgttggtatt gatgcgcctg acttcaatat tactcttgaa
    10201 ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccgtc cgtctgggcg
    10261 tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct cgcatttctg
    10321 cctttcgaaa aagcgtttta tgacaaatac aacgtaccgt gccgctttat cggtcatacc
    10381 atggctgatg ccatgccatt agatccagat aaaaatggtg cccgtgatgt gctggggatc
    10441 ccttacgatg cccactgtct ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg
    10501 cttagtgccg atttcctgaa aacggcccag cttttgcgcc agacatatcc ggatctcgaa
    10561 atcgtggtgc cgctggtgaa tgccaaacgc cgtgagcagt ttgaacgcat caaagctgca
    10621 gtcgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc
    10681 agtgatgcgg cgctactggc gtcgggtacg gcagcactgg agtgtatgct ggctaaatgc
    10741 ccgatggtag tgggatatcg catgaagcct ttcaccttct ggctggcgaa gcgactggtg
    10801 aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt caaagagtta
    10861 ttgcaggaag agtgtgagcc gcaaaaattg gctgcggcgc tgttaccgct gttggcgaac
    10921 gggaaaacca gccatgcgat gcacgacacc ttccgcgagc tgcatcagca gatccgctgc
    10981 aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc gaatttgtct
    11041 atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg
    11101 cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg ctgaatgatt
    11161 ccaaaaagct gagcgaaaaa cgccgtctgg cgctctgtga agagatcaaa gagaaagcgt
    11221 tgagctggag tctgggccgc gcggaacctc acgaaatcga cgaactgaat attttacatg
    11281 cgaccatgct ggcgatgcag cgtgccgtcg ctggactgca tattgcgccg gaatatgtgt
    11341 tgattgatgg taaccgctgc ccgaaattac cgatgccttc gatggctgtg gtgaaaggcg
    11401 atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg
    11461 aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacac aaagggtatc
    11521 caaccgcttt tcatctggaa aaactggctg aacacggcgc gaccgaacac catcggcgca
    11581 gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt cgagattaag
    11641 taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg cacagtgact
    11701 actcgatgat cgatggcctg gccaaaaccg caccgctggt aaaaaaggcg gcagcgttgg
    11761 gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg aagttctacg
    11821 gagcgggaca tggcgcaggg attaagccca tcgtcggggc ggattttaac gtccagtgcg
    11881 acctgctggg tgatgagtta actcacctga cggtgctggc ggcgaacaat accggctatc
    11941 agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga
    12001 tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt tccggcgggc
    12061 gcatgggcga cgttggacgc agtcttttgc gtggtaacag cgcgctggta gatgagtgtg
    12121 tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc cgcaccggca
    12181 ggccggacga agaaagctat ctgcacgctg cggtggaact ggcggaatca cgcggtttgc
    12241 ccgttgtggc gaccaacgac gtgcgcttta tcgacagcag cgactttgac gcacacgaaa
    12301 tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg cgtaactatt
    12361 cgccgcagca atatatgcgt agcgaagagg agatgtgcga gctttttgcc gacattcccg
    12421 aagcccttgc caacaccgtt gagatagcca aacgttgtaa cgtaactgtg cgtctgggtg
    12481 aatacttctt gccgcaattc ccgaccgggg acatgagtac cgaagattat ctggtcaagc
    12541 gtgcaaaaga aggcctggaa gagcgtctgg catttttatt ccctgacgag gaagaacgtc
    12601 ttaagcgtcg tccggagtat gacgaacgtc tggatactga acttcaggtt atcaaccaga
    12661 tgggcttccc gggttacttc ctcatcgtta tggaatttat ccagtggtcg aaagataacg
    12721 gtgtaccggt agggccaggc cgtggttctg gtgcagggtc actggtggcc tacgcgttaa
    12781 aaatcaccga tctcgatccg ctggaatttg acctgctgtt cgaacgtttc cttaacccgg
    12841 aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgt gaccaggtta
    12901 tcgaacatgt ggcggacatg tacggtcgcg atgcggtatc gcagattatc accttcggta
    12961 cgatggcggc gaaagcggtg atccgcgacg taggccgtgt attggggcat ccgtacggat
    13021 ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg tatgacgctg gcgaaagcgt
    13081 ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt aaggcgctga
    13141 tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac gccggtgggg
    13201 tggttatcgc gccgaccaaa attactgatt ttgcgccgct ttattgcgat gaagagggca
    13261 aacatccggt cacccagttt gataaaagcg acgttgaata cgccgggctg gtgaagttcg
    13321 acttcctcgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg atcaacaagc
    13381 ggcgggcgaa gaatggcgag cccccgctgg atatcgccgc gatcccgctg gatgacaaga
    13441 aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt gaatcgcgcg
    13501 gcatgaaaga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg atcgcactgg
    13561 tggcactgtt ccgccccggc ccgttgcaat cagggatggt ggataacttt atcgaccgta
    13621 aacatggtcg cgaagagatc tcctatccgg acgtgcagtg gcagcatgaa agcctgaaac
    13681 cggtactgga gccaacctac ggcatcatcc tgtatcagga acaggtcatg cagattgccc
    13741 aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga
    13801 aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg
    13861 gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc gcgggttacg
    13921 gatttaacaa atcgcactcg gcggcctatg ctttggtgtc atatcaaacg ttatggctga
    13981 aggcacatta tccggcagag tttatggcgg cggtaatgac cgccgatatg gacaacaccg
    14041 agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
    14101 atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc gtgtatggta
    14161 ttggcgcgat caaaggggta ggtgaaggcc cgattgaggc catcatcgaa gcccgtaata
    14221 aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa
    14281 accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc
    14341 gcgcggcgct gatgaactcg ctgggcgatg cgttaaaagc ggcagatcaa cacgcgaaag
    14401 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
    14461 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtgttagat ggggaacgtg
    14521 aaacgttagg tctgtacctc acgggacacc ctatcaacca gtatttaaaa gagattgagc
    14581 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
    14641 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
    14701 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
    14761 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
    14821 gctttgatga cttcagcggt gggcttaaaa tgactgctcg cgaagtgatg gatattgacg
    14881 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
    14941 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
    15001 tacatctcta ctatcagagg gcggatgcac gcgcgcgatt gcgttttggc gcgacgtggc
    15061 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
    15121 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
    15181 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
    15241 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
    15301 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
    15361 cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
    15421 ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
    15481 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
    15541 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
    15601 ctgaacgttt taagatgcct atcatcacct ttatcgacac cccgggggca tatccgggcg
    15661 tgggcgcaga agaacgtggt cagtcggaag ccattgcacg caacctgcgt gaaatgtctc
    15721 gcctcggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggt ggtgcgctgg
    15781 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
    15841 cggaaggttg tgcgtccatt ctgtggaaga gcgctgacaa agcgccgctg gcggctgaag
    15901 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
    15961 aaccgctggg cggtgctcac cgtaacccgg aagcgatggc ggcgtctctg aaagcgcagc
    16021 tgctggcgga tctggccgat ctcgacgtgt taagcactga agatttaaaa aatcgtcgtt
    16081 atcagcgcct gatgagctac ggctacgcgt aatcggcaaa tgttctgaaa agggtcactt
    16141 cggtggccct tttttatcgc cacggtttga gcaggctatg attaaggaag gattttccag
    16201 gaggaacaca tgaacatcat tgccattatg ggaccgcatg gcgtctttta taaagatgag
    16261 cccatcaaag aactggagtc ggcgctggtg gcgcaaggct ttcagattat ctggccacaa
    16321 aacagcgttg atttgctgaa gtttatcgaa cataacccac gaatttgcgg cgtgattttt
    16381 gactgggatg agtacagtct cgatttatgt agcgatatca atcaacttaa tgaatatctc
    16441 ccgctttatg ccttcatcaa cacccactcg acgatggatg tcagcgtgca ggatatgcgg
    16501 atggcgctct ggttttttga atatgcgctg gggcaggcgg aagatatcgc cattcgtatg
    16561 cgtcagtaca ccaacgaata tcttgataac attacgccgc cgtttacgaa agccttgttt
    16621 acctacgtca aagagcggaa gtacaccttt tgtacgccgg ggcatatggg cggtaccgca
    16681 tatcaaaaaa gcccggttgg ctgtctgttt tatgattttt tcggcgggaa taccctcaag
    16741 gcggacgtct ctatttcggt caccgagctt ggttcgttgc tcgaccacac cgggccacac
    16801 ctggaagcgg aagagtacat cgcgcggacg tttggcgcgg aacagagtta tatcgttact
    16861 aatggaacat cgacgtcgaa caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg
    16921 ctgttgatcg accgcaattg tcataaatcg ctggcgcatc tgttgatgat gaacgatgta
    16981 gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga ttctgggtgg gatcccgcgc
    17041 ggtgaattta ctcgcgacag catcgaagag aaagtcgctg ccaccacgca ggcacaatgg
    17101 ccggttcatg cggtgatcac caactccacc tatgatggct tgctctacaa caccgactgg
    17161 atcaaacaga cgctggatgt cccgtcgatt cacttcgatt ctgcctgggt gccgtacacc
    17221 cattttcatc cgatctacca gggtaaaagt ggtatgagcg gtgagcgtgt tgcgggaaaa
    17281 gtgatcttcg aaacgcagtc gacccacaaa atgctggcgg cgttgtccca ggcgtcgctg
    17341 atccacatta aaggcgagta tgacgaagag gcgtttaacg aagcctttat gatgcatacc
    17401 accacctcgc ccagttatcc cattgttgct tcggttgaga cggcggcggc gatgctgcgt
    17461 ggtaatccgg gcaaacggct gattaaccgt tcagtagaac gagcgctgca ttttcgcaaa
    17521 gaggtccagc ggctgcggga agagtctgac agctggtttt tcgatatctg gcaaccgccg
    17581 caggtggatg aagccgaatg ctggcacgtt gcgcctggcg aacagtggca cggctttagc
    17641 gatgcggatg ccaatcacat gtttctcgat ccggttaaag tcactatttt gacaccggga
    17701 atggacgagc agggcaatat gagcgaggag gggatcccgg cggcgctggt ggcaaaattc
    17761 ctcgacgaac gtgggatcgt agtagagaaa accggccctt ataacctgct gtttctcttt
    17821 agtattggca tcgataaaac caaagcaatg ggattattgc gtgggttgac ggaatttaag
    17881 cgctcttacg atctcaacct gcagatcaaa aatatgctgc ccgatctcta tgcagaagat
    17941 cccgatttct accgcaatat gcgtattcag gatctggcgc aagggatcca taagctgatt
    18001 cgtaaacacg atcttcccgg tttgatgttg cgggcatttg ataccttgcc ggagatgatc
    18061 atgacgccac atcaggcatg gcagagacag attaaaggcg aagtagaaac cattgcgctg
    18121 gaacaactgg tcggtagagt atcggcaaat atgatcctgc cttatccacc cggcgtaccg
    18181 ctgctgatgc cgggagaaat gcttaccgaa gagagccgca cggtactcga ttttctactg
    18241 atgctttgtt ccgtagggca acattacccc ggttttgaaa cggatattca cggcgcgaaa
    18301 caggacgaag acggcgttta ccgcgtacga gtcctaaaaa tggcgggata acttgccaga
    18361 gcggcttccg ggcgagtaac gtgctgttaa caaataaagg agacgttatg ctgggtttaa
    18421 aacaggttca ccatattgcg attattgcga cggattatgc gatgagcaaa gctttctact
    18481 gcgatattct tggtttcacg ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga
    18541 aaggggattt ggcgcttaat gggcaatatg tgattgagct tttctcattt ccgttcccgc
    18601 cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca tctggctttt agcgttgatg
    18661 atatcgatgc ggcagtggcg caccttgaaa gccataacgt gaagtgtgaa gccatccgtg
    18721 tcgatccata cacgcaaaaa cgcttcacgt tctttaacga tccggacggg ctgccgttgg
    18781 aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg
    18841 ctactgtaaa atcgcaccat catgacactc acgctcaata gacaacttct ctcctcacgc
    18901 cagattctgg tggcctttag cggcggcctt gattctactg tcttactgca tcaactggtg
    18961 cagtggcgga cggaaaatcc gggcgtcact ctgcgcgcta tccatgtgca tcacggttta
    19021 agcgccaatg ctgatgcctg ggtgaaacat tgtgaaaaca tctgccaaca gtggcaggtg
    19081 ccgctggtgg tcgaacgtgt gcagcttgcg caagaggggc taggtattga ggcccaggcg
    19141 cggcaggcac gttatcaggc atttgcccgt accttgttgc ccggtgaagt gctggttaca
    19201 gcgcaacatc tcgacgatca atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc
    19261 cctgccgggc tttcggctat ggcggaagtt tcggagtttg ccggaacacg gcttatccgc
    19321 ccgttgcttg cccgcacgcg gggggaactg gagcagtggg cgcgtcagta tgatttacgc
    19381 tggattgaag acgaaagtaa tcaagacgat tcctacgatc gtaacttctt gcgcctgcgc
    19441 gtagtgccgt tattgcagca gcgttggccg cattttgccg aagcgacggc ccgcagcgcc
    19501 gcactttgcg ccgaacaaga gagcctgctg gatgaactgc tggcggatga tttagcgcac
    19561 tgccaaacgc aacagggaac gttaaagatt gcgccaatgc tggcaatgag cgatgcccgt
    19621 cgcgcggcga ttatccgccg ctggctggca gggcagaatg cacctatgcc ttcccgcgag
    19681 gcgctggtgc ggatctggca ggaagtggcg ctggcgcggg aagatgcttc cccctgttta
    19741 cgtttgggcg cgtttgaaat ccgccgctat cagtcgcaac tgtggtggat taaatccgtt
    19801 gccgggcaaa gcgaaaccat tgtgccgtgg cagacgtggc tacaaccgct ggaattaccg
    19861 gcggggctgg gaagtgtaca gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac
    19921 gaagcggtca gcgtgcgttt caaagcgcca ggactgctgc atattgtcgg gcgcaacggt
    19981 ggacgtaagc tgaagaaaat ctggcaagag ttaggcgttc cgccgtggct acgtgacacc
    20041 acgccactgc tgttttatgg cgaaacgctg attgcggcgg caggggtatt tgtgacgcaa
    20101 gaaggtgtgg ctgtaggtga gaatggcgtc agttttgtct ggcagaaaac gcttagtcaa
    20161 gtgaaagccg gataagacgc atcaaacgtc gcatccggcg aaagtcaatc aggactcgct
    20221 caccaccacc gtaccgattt ccgggtggct aaagctggta attttatcca gacgcagctc
    20281 gcgggttgca ccagcggcct cgacgaccag atactccaca tttttgcggg agactaaatc
    20341 gctggccttc gcctgcaatt tttcgccatc tttcagctca agtgtcagca ttaaatgatg
    20401 ctggcaggcg agctcaagat tatcgtaatc atcacaattg attggttgat acgtatcatt
    20461 cattgacata atcgctcacc agtaagtttg ccgcagcgta tgctgctttt tccctgacag
    20521 cctcagaaag ggcgtcgtcg gcagccattt cattcagcac tttcaaaacg cagcccagcg
    20581 cgtccggaac gtatcctaag tctccgctgg cgatttccgc gtaccgcttg cgtattaact
    20641 cacaatattt ttccacatgc cctcctgtca gcactctgac ttaaccgtgg atgcaagtct
    20701 aagcctacga agataaactc tgtttcgcaa ggtgactata ccacactcat ttctgcaata
    20761 tcagcgccgc aactgcacgt attccgttac aatggcctcc tgattcgaaa ggagttttct
    20821 tatggcgctt aaagcgacaa tttataaagc gacggttaat gtggccgatc tcgaccgcaa
    20881 ccagtttctc gatgcctctc tgacgttggc gcgccatcct tcagaaaccc aggagcgtat
    20941 gatgctgcgc ctgctggcgt ggctaaaata tgccgatgaa cgtctgcaat ttacccgtgg
    21001 tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac gatcatttgg gtattgattt
    21061 gtggattgag ctggggctgc cggatgagcg acggattaag aaagcctgca cccaggccgc
    21121 agaagtggcg ctgtttgcct ataatagtcg ggcggcgcaa atctggtggc agcaaaatca
    21181 gagcaactgt gcgcagtttg ccaatctttc cgtctggtat ctggacgacg agcagctggc
    21241 gaaagtgagc gcctttgccg atcgtaccat gacgctgcag gcaacgattc aggatggtgt
    21301 tatctggtta tcggatgata agaataatct ggaagtgaac ttaaccgtct ggcaacaacc
    21361 ttcatgattg tgatttcccg acatgttgct atccctgatg gcgagtttga gatcaccgcc
    21421 attcgtgcgc agggcgcggg cgggcagcat gttaataaga cctcaacggc tattcatctg
    21481 cgttttgaca ttcgggcgtc cagcctgcca gagtattaca aagagcgtct gctcgccgcc
    21541 agccatcatt tgatcagcag tgacggagtg attgtcatta aggcacagga ataccgcagt
    21601 caggaactga accgcgaagc ggcgctggcc cggctggtgg cagtgattaa agatttaaca
    21661 acagaacaaa aagcccgaag acctacgcgg cccacccgtg catcgaaaga gcgcaggctg
    21721 gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg gcaaagtgcg cagcggtcgg
    21781 gagtaaaaag aaggaatgga tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag
    21841 cctttttact ctgatgggat gtaataatcg ggccgaagtc gatacgcttt ccccggcgca
    21901 ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc gtgctgccgt gtgctgattg
    21961 cgaaggaatc gaaacctctc tgttcctcga aaaagatgga acctgggtga tgaatgaacg
    22021 ttatctgggg gcacgtgaag agccttcctc tttcgcttcc tacggtacat gggcgcgaac
    22081 tgccgacaag ctggtattaa ccgacagcaa aggtgaaaag tcatattatc gagcgaaagg
    22141 ggatgcactg gagatgctcg atcgtgaagg taatcccatt gaatcgcagt tcaactacac
    22201 gctggaaccg gcaaaatcga gcatgccaat gacgccgatg accctgcgtg gcatgtattt
    22261 ctatatggct gatgcggcga cctttactga ttgcgcgact ggaaaacgtt ttatggtggc
    22321 gaataacgca gagctggagc gtggctacct ggctgcgcgc ggcaacggcg aaaaaccggt
    22381 gttactgtca gtagaaggtc actttacgct tgaggctaat ccggataccg gtgcgccgac
    22441 caaagtatta gcgcccgata cggcaggtaa attttacccg aaccaggatt gcagtagttt
    22501 ggggcagtaa cccgtcttga ggcagaaaca aatgaaatat tgcctcatgc gctgcgctta
    22561 tcaggcctaa agcatacaat gcaatatttt gaattagcac gattttgtag gccggataag
    22621 gcgttcacgc cgcatccggc attttgcgca cgaggctggc ttttatcact gagtcagtcc
    22681 aacaaagcga cttgctttaa tataaatccc tggcttcagg tgcccaatat actgtaactg
    22741 cgtttctggt ttaaatgcag agacatcacc actacgcata tgcatcagat cggcagggct
    22801 aatccagcct gattgcgcca gcgtaagcgg atgcccggct ttggcaaatg catccgtgac
    22861 aaattccgaa caaaaccacg actttttgtc tccttcgcca acgttactta actgcgcttt
    22921 cgccaggccg ctgacgcact gttggcgaaa atcctcggag aacggattca gtgagcacat
    22981 ctggcgagtc accataaatg gaataaattc gacaatgccg cgatagttat aaccgctatc
    23041 tttaatttta ttggcgaacg cggtgatttc tgtggcttgt tgcggggtaa ggtccgggac
    23101 tcgtaagacg aaaagcttat cactgtgctt catggctttt ttaagggaaa caatctggac
    23161 gccagcgcct gtcgcttcgg caacgttatt atttcccaaa tagattgcaa cgtgactcac
    23221 agaggaagtg ctgaatacgc ggataccaaa tgaggttacc ccaaggcttg aggagaaaag
    23281 caaatcgccg ggtttgagat ctggttccgt tatttctttt attgattgtt cggtgaaaga
    23341 gctttgatgc tggaatttaa cagcccatgt tttcgcctcg gcatttaccg ctgtggctga
    23401 tgagtctggc tggctgatat caacggtaca ggccgaaagt aaaagaaagc aggggagaag
    23461 caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa aagggccccg
    23521 aaattcagga ccctttctgg catcagcctt taatctgttt caccagataa tcgacgatgt
    23581 caccagtctt aattaactgt ttctcgccgt tacgacgata tttatattcg atatcatcgt
    23641 tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag
    23701 caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcgc
    23761 gcagttcgct gtacagtttc tcagcaagtt cctgtacgcg gaaggatttg tgcatgttca
    23821 ttggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt
    23881 cgtagttctg ctcaatcgcc gctgctacca cacgcgttac gccgataccg tagcaaccca
    23941 tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt
    24001 acttggtacc cagctggaag atgtgaccga cttcgatacc acgtttgatc agcagcgtac
    24061 cctgaccatc cgggcttgga tcgccagcca ccacgttgcg gatatcagca atttccgggg
    24121 tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg ttagcaccag
    24181 cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc ggcatgttta
    24241 ccggacccag tgaacccgga ccggctttaa ccacggcacg aatttcttct tcggtcgcaa
    24301 aagtcagcgg gctggcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtggt
    24361 caccgcgcac cagcagcgca accagcggga agctactgcc ttcaacggct ttaaccagca
    24421 gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggttt
    24481 tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc ggttctttcg
    24541 gcgcgatagc ttctgccagt tcaatgttcg ctgcgtagtc agaagtatcg gagaagacca
    24601 catcgtcttc accgctctgc gccagcacct gaaattcgtg agaggcgcta ccgccgatag
    24661 aaccggtatc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag attttgctgt
    24721 aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta tggaaagagt
    24781 aagcatcttt catcaggaat tcgcgggaac gcatgacgcc aaaacgcgga cgcacttcgt
    24841 cgcggaactt ggtctggatc tgatagaagt tcagcggcag ttgtttgtaa gagctcagct
    24901 cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg aacggacgct
    24961 cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga ccactctctt
    25021 gccacaaatc ggctggctga accaccggca ttaacacctc gatcgcaccg gcgttgttca
    25081 tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg
    25141 tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga
    25201 tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca
    25261 tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa
    25321 gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg
    25381 ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg
    25441 caaccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc
    25501 ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa
    25561 aagctgcttt tcgacctctg cggtaaaaca aaccgccatt tctgccgctg gcgcactttg
    25621 cgcatagctg gcactggcat cgggaagcga ttcagcaaag gggagatacg gtttgatatc
    25681 cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca cgctgtcttt
    25741 atggcaaaca acctctttca gctctaccag cgacatacca atcgggttgg ggcgaaaagt
    25801 ggagcgcgta gcgaaaacac ccattctggc attaccgccg agacgaggcg gacgcacagt
    25861 cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata aatggctgaa
    25921 tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggga gcaatgagat gtagttcgcc
    25981 gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt tatagggcga
    26041 gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtgat gttaagcgca
    26101 gaaccgatac atacagcctg acgatagcaa cctggcgtac cgctggtgac ttcgcagcta
    26161 tgcagtaata ccgcattggc tttcattttc gaggcgttga tttgcatccg cttacgtgcg
    26221 gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc actgacttca
    26281 ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc
    26341 gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc agtgctttga
    26401 acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa
    26461 cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat
    26521 caggcgcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg
    26581 tggaaacagc ctaaatatta ccagccttta acagcgccgc cgttaaacac tttgtttgct
    26641 gcttcgtaaa cttcgtcaga ctgataagcc tgaacgaatt tcttcacgtt ttcggcgtct
    26701 ttgttgtctt cacgcgttac gatcaggttt acgtacgggg actctttatc ttcgacaaag
    26761 ataccgtctt tcgctggagt caggccaatc tggctggcat aggtggtatt gataactgcc
    26821 agagcgattt gcgcgtcgtc cagagagcgc ggcagctgcg gtgcttccag ttcaacaatt
    26881 ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct
    26941 ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag gttagttggg
    27001 tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg
    27061 gagtaaccag caatcggata aacaaatgtg ttgcctactg cgaccagttt gtagccacga
    27121 tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct
    27181 ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc aacgtccagg
    27241 ccatatttgt ctttcgcaac tttctgcgcg acttctgcaa cctgctgttc ggcaccaaca
    27301 atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc
    27361 agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat
    27421 tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg
    27481 cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag taccgtattc
    27541 atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc taaaccaccg
    27601 gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt aatagttgcc
    27661 gcattcacca gacccggcaa cgcttccggt aacagcactt tacggacgat ctgcattggc
    27721 gtggcaccca ttgcgcggga ggcttcaatt aacccggttg ggatctccag aagagcgttc
    27781 tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat cgctgcctgc
    27841 aatccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac cagcaagata
    27901 atgaacggga tggaacggaa aatgttcaca atcgcagaaa tggtacgata caacttcgcg
    27961 ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc
    28021 acaaagccaa aaaaaccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc
    28081 agcagccaca tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg
    28141 cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcataat
    28201 gccgaactta acgccaccgg cgtaatccat ctgcgcgcta ataatgttgt tgttgacgtt
    28261 gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag
    28321 acgcagcatc ggcacgcagt cagtaaatgg ctccgcttgc agacgttcct ggtaatcttc
    28381 cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg
    28441 cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga tgaccgccac
    28501 gcaatcacaa atgcgcttca caacgtccat ttcgtgagtg atcaacagaa tcgtcaaacc
    28561 cagacggcgg ttgatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag
    28621 cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg cacgggcaat
    28681 cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat gcttatcgcc
    28741 aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt
    28801 gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa
    28861 atgctggaaa atcataccaa tctggcgacg agctttggtc aactcggatt ctgacagcgt
    28921 ggtcagttcc tggccatcga ccagcacgct accctcggtt gggcgctcca gcaggtttac
    28981 acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc
    29041 agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa
    29101 cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc
    29161 gtggcatttc gaatgcctgg aacgggcaac gccgtcaatg aaatggatgt taaggcatcc
    29221 agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga
    29281 tactagcgtc acatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat
    29341 ttttcttgac cgtgatggca ccattaatgt cgatcacggt tatgtccatg agatcgacaa
    29401 ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc
    29461 gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttactg aagcacagtt
    29521 tgaaacgctg accgagtgga tggactggtc gctggcggac cgcgatgtcg atctggatgg
    29581 tatctattat tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc aggtctgcga
    29641 ctgccgcaaa ccacatccgg ggatgttttt gtcagcacgc gattatttgc atattgatat
    29701 ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg ctgcggcgag
    29761 cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acgcctgaag cagaaaacgc
    29821 ggcggattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa
    29881 accggcgtaa tgattaaaag atgagcggtt gaaataaaaa tgcatttttc cgcttgtctt
    29941 cctgagacga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
    30001 aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca gggttg
//
LOCUS       DAOFSV010000046        29978 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.046, whole genome shotgun sequence.
ACCESSION   DAOFSV010000046 DAOFSV010000000
VERSION     DAOFSV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.046"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            133..696
                     /locus_tag="QBH27_002167"
     CDS             133..696
                     /locus_tag="QBH27_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDQ7014477.1"
                     /translation="MLKKTLLSMFATALLSGVAFNALADDPNQGSGKITFKGEVIDAP
                     CSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSS
                     ASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNF
                     KARMESLGRTATPGNVQAQANYVLDYK"
     gene            781..3303
                     /locus_tag="QBH27_002168"
     CDS             781..3303
                     /locus_tag="QBH27_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ7014478.1"
                     /translation="MNNKNTFTGDTLSHAIKNALSGVVCSLLFVLPAHAVEFNVDMID
                     VEDRENIDISRFEKKGYIPPGRYLARVQINKNMLPQALILEWVKADNESGSLLCLTKE
                     DLTSFGLNTEFIESLQTIAGRECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQKQNSDNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYADNAR
                     MMVDTDGISGVEINSNRTVTNGLGIAVVPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSILHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3319..4059
                     /locus_tag="QBH27_002169"
     CDS             3319..4059
                     /locus_tag="QBH27_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ7014479.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRGYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRTKEQNVMQIAMQSRLKLFWRPKAIELKEGQMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVTPFASATQYLNNLPSTFQLGFVAD
                     YGGLEMFKVECNSVQSLCQSSPAKKGKI"
     gene            4056..5105
                     /locus_tag="QBH27_002170"
     CDS             4056..5105
                     /locus_tag="QBH27_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDQ7014480.1"
                     /translation="MITLFRLLAILCLFYNVSVYAVNCYQDQRGGNTQIRGTLPSFRI
                     PENAQPGQKIWESDDVNVTVYCDNATGWRADDPAENIYAWIKMPAINSADILNNPYLT
                     FGVTYNGVDYEDSDVGIDTFACLDKYEQYYGGIYHDPVCNGSTLQKNVTFNARFRAYV
                     KFKARPAVDQTWDFGVVNVLQFDGEGGANLAADNKNLRYIIDGLNNILFLDCSVDVRI
                     FPESQIVNFGQISANSIATYRPKAAFSVSTIKDVAADCTEQFDVVTSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNKYKRFTTYIPGQTGAMVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(5159..5812)
                     /gene="ribB"
                     /locus_tag="QBH27_002171"
     CDS             complement(5159..5812)
                     /gene="ribB"
                     /locus_tag="QBH27_002171"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDQ7014481.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5917..6065)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6187..6486
                     /gene="ubiK"
                     /locus_tag="QBH27_002172"
     CDS             6187..6486
                     /gene="ubiK"
                     /locus_tag="QBH27_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDQ7014482.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPAAELQSPPAIPPVDKAE"
     gene            complement(6521..6721)
                     /gene="glgS"
                     /locus_tag="QBH27_002173"
     CDS             complement(6521..6721)
                     /gene="glgS"
                     /locus_tag="QBH27_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDQ7014483.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            6991..7620
                     /locus_tag="QBH27_002174"
     CDS             6991..7620
                     /locus_tag="QBH27_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDQ7014484.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7647..9308
                     /locus_tag="QBH27_002175"
     CDS             7647..9308
                     /locus_tag="QBH27_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDQ7014485.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9548..9607
                     /gene="ibsE"
                     /locus_tag="QBH27_002176"
     CDS             9548..9607
                     /gene="ibsE"
                     /locus_tag="QBH27_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDQ7014486.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(9727..11160)
                     /gene="hldE"
                     /locus_tag="QBH27_002177"
     CDS             complement(9727..11160)
                     /gene="hldE"
                     /locus_tag="QBH27_002177"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDQ7014487.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(11208..14048)
                     /gene="glnE"
                     /locus_tag="QBH27_002178"
     CDS             complement(11208..14048)
                     /gene="glnE"
                     /locus_tag="QBH27_002178"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDQ7014488.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     ALLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14071..15372)
                     /gene="ygiF"
                     /locus_tag="QBH27_002179"
     CDS             complement(14071..15372)
                     /gene="ygiF"
                     /locus_tag="QBH27_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDQ7014489.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15614..16234
                     /locus_tag="QBH27_002180"
     CDS             15614..16234
                     /locus_tag="QBH27_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDQ7014490.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINRLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16298..17536
                     /gene="cca"
                     /locus_tag="QBH27_002181"
     CDS             16298..17536
                     /gene="cca"
                     /locus_tag="QBH27_002181"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDQ7014491.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVSLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDSLFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(17616..18437)
                     /gene="bacA"
                     /locus_tag="QBH27_002182"
     CDS             complement(17616..18437)
                     /gene="bacA"
                     /locus_tag="QBH27_002182"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDQ7014492.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGENKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(18528..18896)
                     /gene="folB"
                     /locus_tag="QBH27_002183"
     CDS             complement(18528..18896)
                     /gene="folB"
                     /locus_tag="QBH27_002183"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDQ7014493.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19001..19618
                     /gene="plsY"
                     /locus_tag="QBH27_002184"
     CDS             19001..19618
                     /gene="plsY"
                     /locus_tag="QBH27_002184"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDQ7014494.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(19631..20563)
                     /gene="ttdR"
                     /locus_tag="QBH27_002185"
                     /gene_synonym="dan"
     CDS             complement(19631..20563)
                     /gene="ttdR"
                     /locus_tag="QBH27_002185"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDQ7014495.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            20770..21681
                     /gene="ttdA"
                     /locus_tag="QBH27_002186"
     CDS             20770..21681
                     /gene="ttdA"
                     /locus_tag="QBH27_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDQ7014496.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            21678..22283
                     /gene="ttdB"
                     /locus_tag="QBH27_002187"
     CDS             21678..22283
                     /gene="ttdB"
                     /locus_tag="QBH27_002187"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDQ7014497.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22331..23794
                     /gene="ttdT"
                     /locus_tag="QBH27_002188"
     CDS             22331..23794
                     /gene="ttdT"
                     /locus_tag="QBH27_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDQ7014498.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHAMLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(23837..24850)
                     /gene="tsaD"
                     /locus_tag="QBH27_002189"
     CDS             complement(23837..24850)
                     /gene="tsaD"
                     /locus_tag="QBH27_002189"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDQ7014499.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25088..25303
                     /gene="rpsU"
                     /locus_tag="QBH27_002190"
     CDS             25088..25303
                     /gene="rpsU"
                     /locus_tag="QBH27_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDQ7014500.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25414..27159
                     /gene="dnaG"
                     /locus_tag="QBH27_002191"
     CDS             25414..27159
                     /gene="dnaG"
                     /locus_tag="QBH27_002191"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDQ7014501.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27354..29195
                     /gene="rpoD"
                     /locus_tag="QBH27_002192"
     CDS             27354..29195
                     /gene="rpoD"
                     /locus_tag="QBH27_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDQ7014502.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(29273..29779)
                     /gene="mug"
                     /locus_tag="QBH27_002193"
     CDS             complement(29273..29779)
                     /gene="mug"
                     /locus_tag="QBH27_002193"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDQ7014503.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            29904..29976
                     /locus_tag="QBH27_002194"
     tRNA            29904..29976
                     /locus_tag="QBH27_002194"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29937..29939,aa:Ile,seq:cat)
BASE COUNT         7566 a         7935 c         7344 g         7133 t
ORIGIN      
        1 ttaaccaatc ttcaatataa acatatctgg ccacctctaa ctatggtaat ctctctatgc
       61 aattaatatt aaattagacc tgtattaatt gtcccattca cagcctatcc taatctataa
      121 aggattatag tcatgttaaa aaaaacattg ttatctatgt tcgcaaccgc attgttatct
      181 ggcgttgctt ttaacgcgct tgctgatgac ccaaatcagg gttctggtaa aataacattt
      241 aaaggtgaag ttatcgatgc gccttgttct attgctcctg gtgatgaaga tcagacaata
      301 aacctcggtg aagttgctga tactgtatta aaaagcggac agaaatctct tcctgtagat
      361 gtcaccattc acttgcagga ttgtatttta tctgacggca ctaacactgt tgataaagtc
      421 aaaatcacct ttagttctgc cagtgttgac gctaccgact ccaacctgct taaaaacact
      481 ctggaaggta acatcggcgg cgcaactgat gtaggcgtac gtctggtgaa atcagacaac
      541 accaacgtga ctcttggcac tccaatcact atcaacttcc cgacgactaa ctcctaccag
      601 gagttgaact ttaaagcccg tatggaatct ctgggacgca ccgcgacccc gggtaacgtg
      661 caggcacagg ccaattacgt actcgactac aagtaagcgg caattaatac gcacaacgtc
      721 agacggtaaa gccgatgata tgtcatcggc aactttgggt cttttttgaa atggattttt
      781 atgaacaata aaaacacgtt taccggggat acattatccc acgcaattaa aaatgccctg
      841 tctggcgtag tgtgttcgct actcttcgtt ttacctgccc atgcagtgga attcaacgtt
      901 gatatgattg atgttgaaga ccgtgagaat atcgacatct ctcgttttga gaaaaaaggc
      961 tatatccccc caggcagata cctcgctcgt gtgcaaataa ataaaaatat gttgccacaa
     1021 gcgttaatac tggaatgggt aaaagccgat aatgaaagtg gttcgttact ctgcttaacc
     1081 aaagaagatc tgactagttt cggtcttaat acggaattta ttgaatcatt gcaaaccata
     1141 gctggcagag aatgtctcga tttaagccaa cgtcaggagt taacgacacg gcttgataaa
     1201 gctacgatga tattatcgct aagtgttccc caggcgtggt taaaatacca ggcaacaaac
     1261 tggacgccac cagagttttg ggataccggt atcgccgggt ttatccttga ttacaatgtg
     1321 tacgccagcc aatatgcccc acatcatgga gacagcaccc aaaacgtcag ctcctatggt
     1381 acgttaggct ttaacctcgg cgcatggcgc ttacgtagcg attaccaata taatcagaat
     1441 tttgctgatg gacgctcggt aaaccgcgac agcgaatttg cgcgaactta tctgtttcgc
     1501 cctatcccct cctggtcgtc aaaattcact atgggccagt acgacctgag ctccaatctt
     1561 tacgatacct tccactttac tggcgcatcg ctggaaagtg atgaaagcat gctgccgcca
     1621 gatttacagg gttatgcgcc acaaattacc ggcatcgcgc agaccaacgc gaaagtaact
     1681 gtggcacaaa atggtcgtgt actttatcaa accactgtcg cgccaggccc ttttactatt
     1741 tctgatttgg ggcaatcgtt tcaggggcag ctggatgtca cagtggaaga agaagatggc
     1801 cgcaccagca ccttccaggt tggatccgca tccattccct atttaacccg taaagggcaa
     1861 gtgcgctata aaacgtcgct gggaaaaccg acatccgtcg ggcataacga tatcaataat
     1921 ccctttttct ggacggcgga agcctcctgg ggctggttga acaatgtgtc gctgtatggt
     1981 ggcggcatgt tcaccgctga tgattatcag gctatcacta ccggtattgg ctttaatctt
     2041 aaccaattcg gttcgctttc ttttgatgtc accggagcag acgcgtcttt gcagaaacaa
     2101 aatagcgaca atctgcgtgg ttacagctat cgcttcaact atgcaaagca tttcgaatcg
     2161 acaggtagtc agattacctt cgcgggttat cgcttctcag ataaagatta cgtgtcgatg
     2221 agtgagtacc taagctcgcg taatggcgat gagtcaattg ataatgaaaa agagagttat
     2281 gtcatttcct tgaaccagta ctttgaaaca ctggaattaa actcttatct caacgttaca
     2341 cgcaatactt attgggacag cgccagcaat accaactact ccgtatctgt aagcaaaaac
     2401 tttgatattg gcgatttcaa aggtatctct gcatcgctgg cagtaagtcg aatccgctgg
     2461 gatgacgacg aagagaatca atattacttc tctttctctc tacctttaca acaaaaccgc
     2521 aacatctcct acagtatgca gcgtacggga agcagtaata cttcgcagat gatttcctgg
     2581 tacgattcat cagatcgcaa caatatctgg aatatttcag cgtcggcaac ggacgacaat
     2641 atacgtgatg gcgaaccaac actgcgcggc agctaccagc actattcacc gtggggacgt
     2701 ctgaacatta acggcagtgt acagccgaat cagtacaatt ctgttaccgc aggctggtac
     2761 ggttcactta ccgctacacg tcatggtgtc gcccttcacg attatagcta tgctgataac
     2821 gcccgcatga tggtcgatac cgacggcatc tccggcgttg aaatcaactc taaccgaact
     2881 gttaccaacg ggctgggcat tgccgttgta ccttcgttat cgaactacac cacctccatg
     2941 ttgcgggtga acaataacga tctgccagaa ggtgtcgatg tcgaaaactc ggttattcgt
     3001 actacgctca cccagggtgc catcggctac gcaaaactga atgccaccac cggataccaa
     3061 atcgtcggcg ttattcgtca ggaaaatggc cgcttccctc cactaggtgt gaatgtcacg
     3121 gataaagcga caggtaaaga tgtgggcctg gtcgcggaag acggcttcgt ttatctcagc
     3181 ggtattcagg aaaacagtat tctgcattta acctggggtg ataatacctg tgaagtcacg
     3241 ccgccaaacc aaagtaacat tagcgaaagc gcaataattt taccttgtaa aacagtcaaa
     3301 taattcagga tgaatataat gaaatcacca ttaatcacaa caggaatgat ttgtttactg
     3361 gggatctgca attttgccca ggcgactgtc tcccccgaca ggacacgtat catttttaat
     3421 gcttcgaata aaagcgcaac ggtccgtctg actaaccaaa gtaaaatcga tccctatctg
     3481 gctcagtcgt ggattgaaga tgccagcggt aaaaaaacgc gtggttacat atcaacactg
     3541 ccgccgatgg aacgtattga gccggatgaa caggtacaaa ttcgcctgat ggcgctggcc
     3601 tcattaaaca acttaccgca ggacagagaa acgctgttct actataacgt gcgggaaatt
     3661 ccgcctcgca ctaaagaaca gaatgtgatg caaatcgcca tgcagagtcg actgaaatta
     3721 ttctggcgtc cgaaagcaat cgaattaaaa gaagggcaga tggtgccttt acaaaaagtc
     3781 acaataacac ggaccgctgc agggctgaca ctcaataatc caacgcctta tcacatcacc
     3841 gtcgggtata tcggtactaa cggcaagaca ctgatgcccg gtgcagacag cattatggtg
     3901 actccgtttg cttctgcaac tcaatacttg aacaacttgc cctcaacttt ccagttaggc
     3961 tttgttgctg actatggtgg actggagatg tttaaagtcg aatgtaattc cgtccaatcc
     4021 ctctgccaaa gctctcctgc taagaaggga aaaatatgat tacgcttttc cgtttactgg
     4081 cgattctttg ccttttttat aacgtttcag tttatgctgt aaattgctat caggatcaaa
     4141 gaggtggtaa tacacaaata cgtggtactt taccttcctt cagaattcca gaaaacgccc
     4201 agccgggaca aaaaatttgg gaaagcgatg acgttaatgt tacggtatat tgtgataacg
     4261 cgacaggatg gagagctgat gatcccgcag aaaatattta tgcctggatt aaaatgccgg
     4321 ctataaatag tgcagacatt ttgaataacc catacctgac attcggtgta acctataatg
     4381 gtgtggacta tgaagatagc gatgtaggaa ttgatacttt cgcctgtctg gataagtatg
     4441 aacagtatta tggtggtatt tatcatgatc cagtttgtaa tggcagcacc ctacaaaaaa
     4501 atgtcacgtt taatgcccgt tttcgcgcat atgtgaaatt taaagcacgt ccagcagtgg
     4561 atcaaacctg ggattttggc gtggtaaatg ttctgcaatt tgatggtgaa ggtggtgcta
     4621 acttagcagc agataataaa aatctacgtt atattattga tggattaaat aatattttat
     4681 ttcttgattg tagtgtcgat gtccgtattt ttccagaaag ccagatcgtt aatttcgggc
     4741 aaatctctgc aaactctatt gctacttatc gacccaaagc tgcatttagc gtatctacta
     4801 taaaagatgt tgctgccgac tgtaccgagc aatttgatgt tgtcaccagt ttttatacga
     4861 cagatacgtt acatgatgat acgcatctgg aaatgggtaa tggtttgtta atgcgtatta
     4921 ctgatcaaaa aacacaagaa gacattaagt ttaataagta caaacgcttc accacatata
     4981 ttccaggtca gactggagcg atggttaccc gtgattacca ggctgaactt tcacaaaaac
     5041 cgggggagac tctcgtttat gggcctttca aaaaagatct catcgtaaaa ataaactata
     5101 actgaacagc aaaatgccgg gaacaccccg gcattttcat atttagtatt tcacgtcatt
     5161 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
     5221 ccatattgtg tttattcgca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
     5281 cattagtcag ctcacacagc acaccagctg gtttaaagcc tgccagcgtc atcagatcga
     5341 tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga
     5401 aaacgtggcc tgggcgattc agatctgaag gttttgcgcc atcggcaatc gctgcgcgaa
     5461 cggtcgtaat acggtcagcg gcagaaacac cggtagtcac accttcagct gcttcaatgg
     5521 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcga
     5581 gttgtttacg gcgatcgtca gtaatgcaca ggcaaacaat accactcccg tggcgaatgg
     5641 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
     5701 cacggtcttc atcatcaagc accatcacac cgcgtccttc acgcagcgca gccagtgcat
     5761 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
     5821 aaacctcact aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag
     5881 ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc
     5941 taaccgtcgg ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc
     6001 tcgcgggctt tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa
     6061 taagcggatt cactataacg ctaatgatta gcgccagcaa cgcatagctt cacataattc
     6121 tggtttatga cttaccctta tcgcactaca atggcactca acacctatca gtacagggaa
     6181 actacaatga ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg
     6241 cctaaaggaa tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa
     6301 gcgcagctga cgcgtctcga tctggtaagc cgcgaagaat tcgacgttca gacgcaagtc
     6361 ctgttacgga ctcgtgaaaa actggcgtta ctggagcagc gcatcagcga actggaagcg
     6421 cgcaataatc cggcagcaga acttcaatct cctccggcta tcccgccagt tgataaagca
     6481 gaataaagca ccacaaaaac gggccagacg gcccgtaata tcagtgttct aattcaagtt
     6541 ctctggtttg catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat
     6601 gttcttcatc catgttacca gtaacggcca gaatatcgac cgggcgacct tctgcgtgca
     6661 ttctggcaaa actacgcgcg agaaaatcga aattatttaa agaattaaga ctatgatcca
     6721 taagcacatc ctccggttat tgcatacctt ttaaggccat atgctttttc tgatttcagt
     6781 attccccaat tagtgtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca
     6841 cactttttag tgaaaaataa aaaaaccata ttaaaagact ttctcttcca gaacttctca
     6901 aacatgtgtt attattaaca aagataaaag taatcaagga aactattcca aagtaaatat
     6961 tgcgtcacta aatggacatt ggagtgacat atgattttat tcgccgacta taacacccct
     7021 tatcttttcg ctatttcctt tgtactcttg atcggccttt tagaaatttt tgcacttatc
     7081 tgcggtcaca tgctctccgg tgctcttgat gcacatcttg accattatga ttccataacc
     7141 acaggtcata ttagccaggc acttcattat ctcaatattg gaagattgcc agctctcgtc
     7201 gtcctctgct tactcgctgg tttttttggt cttattggca tcctgttgca acatgcctgc
     7261 gttacgctct ggcaatcacc actccccaac ctgttcgtgg ttcctgtcag tttactgttt
     7321 acaattattg cagtgcatta taccggtaaa gtcgttgcac cctggattcc acgcgaccat
     7381 agttcagcta ttacagaaga agaatatgtt ggtagcatgg cattaattac cggccatcaa
     7441 gccacttcag gtaacccctg cgaaggaaaa ctcaccgatc aatttggcca aatacattat
     7501 ttgctactgg agcctgaaga aggaaaaact ttcactaaag gagacaaagt gttaattatt
     7561 tgccgacttt ctgcaacgcg atatttggcg gaaaataatc cctggcccca gatattgtga
     7621 tttattaatc aaaaggaaat ttaataatgg atgatatcgt taattctgtg ccctcctgga
     7681 tgtttaccgc gattatcgcc gtatgcattc tgtttattat tggaattatt ttcgccaggc
     7741 tctatcgtcg cgcttcggca gagcaagcat ttgttcgtac tggtttaagt gggcaaaaag
     7801 tggtgatgag cggtggcgca atcgtgatgc cgatctttca tgaaataatt cccatcaata
     7861 tgaataccct gaagctggaa gtcagccgct caaccattga tagcctgatt acgaaagatc
     7921 gtatgcgcgt cgatgtcgtg gttgctttct ttgtgcgggt aaaaccttca gtagaaggga
     7981 ttgccaccgc cgcccagacg ctggggcaac gcaccctgtc gcctgaagac ttacgtatgc
     8041 tggtggaaga taaatttgtc gatgccctcc gtgcaacagc ggcacaaatg accatgcatg
     8101 agttacagga tacccgcgag aacttcgtgc agggagtaca aaataccgta gctgaagatc
     8161 tgtcgaaaaa tggcctggaa ctggaaagcg tttcacttac caactttaac cagacctcga
     8221 aagaacattt caatccgaac aatgcctttg acgccgaagg tttaaccaaa ctgactcagg
     8281 agacggagcg ccgtcgtcgc gaacgtaacg aagttgaaca ggatgtggaa gtcgcagtgc
     8341 gtgagaaaaa ccgtgatgca ctttcgcgca agttggagat tgaacaacaa gaagcgttta
     8401 tgacgcttga gcaggagcag caggttaaaa cccgtaccgc tgagcagaat gcgaaaattg
     8461 cggcttttga agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac
     8521 agattcagga aacggaaatc gaccgcgaac aggccgtccg ctcaagaaag gttgaggccg
     8581 aacgtgaagt tcgcattaaa gagatcgaac agcagcaggt caccgaaatc gctaaccaga
     8641 cgaaatcgat cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta
     8701 accttgcact cgcagaagca gtaagcgccc aacaaaacgt agaaaccact cgccagactg
     8761 ccgaagccga tcgtgctaaa caagtggcgt tgatcgccgc agcgcaggat gcagaaacca
     8821 aagcggttga actgaccgtg cgggcgaaag cagaaaaaga agccgcagaa atgcaggcgg
     8881 cggctatcgt tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac
     8941 aacgtgcgct gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca
     9001 aactggcatt attgcaggcg ctgcctgcgg taattgagaa atccgttgag cctatgaagt
     9061 ctatcgacgg catcaaaatt attcaggtcg atggtctgaa tcgtggcggc gctacgggtg
     9121 atgcaaacac gggtaatgtg gggggcggaa acctggcaga acaagcgtta tcagccgcac
     9181 tctcttaccg cacacaggca ccgctgattg actccttgct caatgaaatt ggcgtttcag
     9241 gcggctcact ggcggcattg acttcatcct taagctcaac aactcccgtc gaagaaaaag
     9301 cagagtaaac cctcttctat gaataatccc tggcccaaaa agccggggat tttttatatc
     9361 tgcgttccgc taaaaggtgc aaatgatcag gctgttgcag cattttgcgt gactgctcgg
     9421 ggaaggcaaa actgcctctg ggaaagcgtt gcgcggggtc cggcgctcat caacaatcgg
     9481 ggagtcagca aggggctgaa acgggaaagc ccctcccgag gaaggggcct tatagaagga
     9541 aagggttatg atgaagttcg tcatcatact ggttgtgttg ttactgttaa gtttcccgac
     9601 ttactaacaa ctcatcagag gggggagaaa tcctccctta cccttgttcc tttactttag
     9661 gttgaaaaaa caacagcgtc aatagacctg cctgctacga agcgagatct gtgaaccgct
     9721 ttccagttag ccttttttat ccagttggat cttcttgatg atgttggtcg tcgagcaacc
     9781 gtcttcaaag ttgagcacca gcacttcgcc accgttggcc cagacttctt tactcccggc
     9841 aatctcttct ggtttatagt cgccgccttt caccagcaga tctggcaaga tcccggcgat
     9901 caagcgctgc ggtgtgtcct cttcaaacga caccacccag tcgaccgctt ccagtgcgcc
     9961 cagcacaatc atacgctgtt cgagcgggtt aacggggcgg gaatcccctt tcagccgttt
    10021 ggtggaggca tcgctgttca cagcgacaat caaacggtca cccagtttac gggcatttgc
    10081 cagataagag acgtgcccgg cgtgcaggat gtcaaagaca ccgttggtca tcaccacttt
    10141 ttcaccgcgt ttacgcgctg ccgctacagc aagcttcagt tcctcttcgg tcatcacacc
    10201 aaagccggta tctgcacgtc cacgtactgc gttttccagc tcgatcggcg aaaccgtgga
    10261 tgtccccagt ttgccgacca ccacaccagc cgccgcattg gcaaagaagc aggcttcttc
    10321 cagcgaatta cccgctgcca gcgttgccgc caggacgcca atcaccgtgt cgcccgcacc
    10381 ggtaacgtca tacacttcct gcgcctgggt tggcatatgc agcggcgctt tacccggttg
    10441 cagcagcgac ataccctgtt cggaacgggt cactaacaga gccgagagtt cgtaatcggc
    10501 aatcagtttc atgccgcgct caacaatctc ttcttcggtc ttacatttac cgacaacagc
    10561 ttcaaattcc gagagattcg gcgttaacag cgtagcgccg cggtagcgct caaaatcggt
    10621 accttttgga tcaatcagca ccggaacacc cgctttacgc gccagttgga tcatctgctg
    10681 tacgcttgcc agcgcacctt tggcgtagtc agaaagcacc agcgcgccaa tcgaactcag
    10741 tgcctgatta atccgctcgt gcagcggctg cggatcaacc ccttcgaaac cttcttcgaa
    10801 atccagacgg atcagctgtt ggttacggga aagtacccgt aatttggtga tagtcggatg
    10861 cgtcggtaca gaaacaaagt cgcatttaac gttgacgtcg gccagagatt tactgagcgc
    10921 gcgcgccgca tcgtcaatgc ccgtcaaccc gaccaggcgt gcattagcac cgagagaagc
    10981 gatattcatc gccacgttag ccgcgccgcc cggacgttct tcgatggtat tcactttaac
    11041 cacgggtacc ggcgcttccg gcgagatacg gctggtggga ccgtaccagt aacgatccag
    11101 catcacatca ccaaccacca tcactcctgc acgttcaaac tctggcagcg ttactttcat
    11161 tcctgtctcc tgagagattc aaaatttgcg cgcgataata ccatacttca ttcttccacc
    11221 agccacttct gccagcttgc ccgcaccagt tcacgctctg cggtgaagca atcctccgac
    11281 acatggcccg gcaattcctg taatgccaga tgatgaagtt catcgcgcag cgtcgtgtaa
    11341 gcacgggtca gcgccatcgc ttcctgctct tccataatgt cgttttgcgc cagtagttcc
    11401 agaatgcgca cgttgtctga ccagcgcgtt aacttcggtt tttcgtgagc gtagcgcaac
    11461 accagatatt gagtaataaa ttcgatatcg gtaattccgc cttcatcggc tttgatatca
    11521 aagcgatcgc gatgtttatt gccgagatgg gcacgcattt tctcgcgcat ttcccgcact
    11581 tctgtttgca gggttttgcc ttcgcgcggc agcgtcataa tctcccggcg tactgcgtca
    11641 aagtgcgcgg tgagctgcgg atcgccgtac actacacgcg cacgcaccag cgcctgatgc
    11701 tcccacgtcc aggcctcgtt tttctgataa tcagcaaatg cttctgcgga tgtcaccagc
    11761 attcccgccg ccccggacgg acgcagtcgt gcatccactt catacaaaat gccggaagag
    11821 gttcgcgtac tgaacagatg cataatgcgt tgcgccagac gcagataaaa ctgtcgcccg
    11881 tcgatttccc gctcgccgtc agtcatcgca tccattgggc aatcatggag gaagatcagg
    11941 tcaagatcgg aactgtagcc taactcccag ccacccagct tgccgtagcc gaccaccgca
    12001 aaaccacgcc cttcgcgttc gttcaggtga tttggcttac cgtagcgggc aaccatttga
    12061 acccacgcct gctgaacgac ggcatcaatc atggcttctg ccagccaggt taagtgatcg
    12121 ctcactttca ttaccggtag cgtaccggcg atatccgctg ccgcaatgcg taacagctgc
    12181 gcctgtttga actgacgcag cgcctcaagc tgttgttctt cgtcatcttc cggcacgcgc
    12241 agcaaatact ggcgcagttc atcacggtag gcatccgtcg ccgttggttg gtagagggta
    12301 tttggatcga gcaattcatc cagcaataat ggataacgcg ctagctggct ggcaatcatc
    12361 ggcgatgcag cacacagaga aatcaaatgc ttaagcgccg cagggaattc actaagcagc
    12421 tcaagataag tggtgcgagt aacaatcccc accagcaagg cggtaatgcg cgacagcgtg
    12481 acagcagcgt cttcacgcgc acagacatca cttagcagat gtggcatcag atggtcgagc
    12541 acctgacgtc ctcgcggccc gatggtgcgc ttatccagct ctttgcggaa atcagcaatc
    12601 agcattagca cctgtttgcg atcatcctcg ctaagatgcg ccagcactgg cgtggtgtca
    12661 tcttcctgca acgcatcctg ccacagctca cgccactgtt ccgacagcga ctcttcctga
    12721 gtttcacttt cgtcgtcgcc aatcaattca ttaaacaccc ggcgcacatt ggccatatgt
    12781 gcggtcagca ccccggtcaa ttgcggccag tcagcaaagt ccatcgccca cgccagccgc
    12841 gcacggttaa gctcatcaga aggaagcgtc tgggtttgtt cgtcgttaat gctttgcagc
    12901 aggttttcca gacgccgcag gaacagatac gccactcgca attgttcagc atcgttttca
    12961 gaaagcagat gcagcgcggc aatggcgctg agcgttggca gtaaagagcg cgattgcagc
    13021 gacggttcgc gcccgccgcg aatgagctgg aacacctgga cgataaattc aatttcgcga
    13081 atgccgcctg cgccaagttt gatgttgtcg gtcaaaccgc gtcgacgcac ttcacgggca
    13141 atcattcctt tcatgttgcg cagcgactga atcacgctga aatcgatgta acgacggaaa
    13201 acaaacgggc gcagcaacgc acgcaactcg ttagcataga cgccgtcgct atcccccata
    13261 atccgcgcct tgaccatcgc gtaacgctcc cagtcgcgcc cctgctcctg gtaataatct
    13321 tccagcgccg caaagctcag caccagcggg ccactttccc caaacggacg cagccgcata
    13381 tccacgcgat agacgaagcc atcctgcgtt ggttgatcca gtactttaat cagccgctgc
    13441 cccatgcggg taaaaaactg cgcgttatcc agttcccggc gtccgccctg ggtacaacca
    13501 tgttccggcc aggcaaaaat cagatcgata tctgaggaga aattcaattc cccgccgccc
    13561 agcttaccca tgcctaaaat cagcagcggt tgcgcttcgc cctgcgcatt gcacggcgtt
    13621 ccccattcgc ggcagcaggc gtcatacagc cagtcacgcg ccgcaacaat cagcgtctcc
    13681 gccagatggc tgagctgctg caatatgctc tcttcagtaa ccagtgccag cgtttgcgcc
    13741 caggcgatgc gcaccataat gcgccgccgg aatagccgca gctcgcgcat taacccagcc
    13801 tcgtcactca cattactgag cgcctcctgc aaccatgcag cgtaatgctg ccattcgtcg
    13861 gcctgcggcg gttggctttc cagttccgtc agccactctg gatgcgcaat cacgctgtcc
    13921 tgcacaaaat cactgaaagt aagtactgac ttcgcctgtg cgctaagtga ttcctcggct
    13981 aaaggctctg gcagccgctc aacaacggtc tgccagtact gctgtaacgg tgaagagagc
    14041 ggcttcataa gcgattttat ccttgcctga ttaacgtttt ccgctgtgca accagaacgg
    14101 ttcctgattg tttgcctcat tacggaaatg ttcaatttca atgcgttgcc cggtcgcaat
    14161 agcgtgacgc agcccctgcc agttctccag ccagtcttgc gcgacgacag gatcgtagta
    14221 acccgccagc aacagtattg agtcaatatc acgcgtcagg cgtggcaatt ggtcacggta
    14281 gcgatcgcct aacggctggc agaaaacgct tttcagttca gcggcatggc gggaaagatg
    14341 gatatcggca aagcgtttga aggaatcgct cattttactc tgcgctttgg catctaaaaa
    14401 cggctgccag gctttgctta ccagccattc ggtcaacgcc agcttcgcca ttgccgtttc
    14461 ggtagagtag accgccgtta cggcagaaac cgcagaagca atggtcgcct cgcattgagt
    14521 cagcagatca cgtaagtgag tgctcgcttt acgcggcaca atgccaccga acagcatcag
    14581 tgtatgacga accaggccaa tggctgccag cacctgctct ttcgccgcat cgttgccgcg
    14641 tacccacagt tcttcatgat actgccattg cgctaacgcc agctcgagcg ccgcttccag
    14701 cccctgttcc acatcagctt ttgccgcaac atgcaaaatg gtggtcggtt tgatttcacg
    14761 cgccggattg ccctgcgcca gatgatagcc acgcgctgct ttgctcaggc tgccctggcg
    14821 taatccggtt tgcgatacca gttggttcgc cagtttcagc accgcacgca tgtcgccgct
    14881 aagcagttcc agttccagct cacagatagg ttcagcaaat tcacccgctt tcacctcacc
    14941 ctgatcgaga gcgatttcaa ttcggctgcc atcgacctct actagccatt tttcgcgata
    15001 aaaatcggtg ctaaacagcg gctgcacgcg ggaagcgaga tcggtaggca attcgccgtt
    15061 cggccagact tccgtcggta actgcgccag gtcgagcgtc ggttcgctca acgccacgtt
    15121 atattccggg cgctggtgta agccgcctgt cactcttcct gcaactttca tggtcatctc
    15181 atagcgaccg ttttcgccac gaatacgcaa gcccatgtcg tgcccacgca gccaattatc
    15241 cggcgtttcg tagtaaatat tcagcaactg cacggggtca tggtgctcgc cgcccagcgt
    15301 attgagatgg tcacgcagcg cctcaacggc actgtgatta acaataaact ttaattcgat
    15361 ttcctgagcc atggccttta cttactggtt atgtcacata agtgacgatg aacggcgaac
    15421 ttaatgcgat cttttttgtc agtagatagt attttgcgcc aaattgccat gcaacgggca
    15481 atttgacggg cgtaaaagtt tgaagcagtg gcaagacctg acacagagga ttccgattga
    15541 tgtcgaatgc tttgcgtagc gacactgatg ccactactat cgttccactt tccatgacaa
    15601 taacgacagc ctgatgccaa aattacgcct gatcggatta actttactcg cacttagcgc
    15661 gactgccgtc tcacacgctg aagaaacgcg ctatgtttcc gacgaactga atacctgggt
    15721 ccgtagcggt ccgggagatc attatcgcct cgtgggcacg gttaacgccg gcgaggaagt
    15781 gaccttatta caaactgacg ccaacaccaa ttatgcccag gtgaaagaca gctctggccg
    15841 tactgcctgg atcccgttga aacaacttag cactgaacca agcctgcgct cccgtgtgcc
    15901 agatctggaa aatcaggtca aaactctgac cgataaactc accaatatcg ataacacctg
    15961 gaatcagcgc accgcagaaa tgcagcaaaa agtggcgcag agtgacagcg tgatcaacag
    16021 gttaaaagaa gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga aaaaggtcga
    16081 tgccgccagc gtacagttgg atgacaaaca gcgcaccatc atcatgcagt ggtttatgta
    16141 tggcggtggc gtgctggggc ttggcttgct gcttggtctg gtactgccgc acctgatccc
    16201 aagccgcaaa cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg cactgtctta
    16261 ttattaggaa ttgtgtattt gacgaaggga tgatggcgtg aagatttatc tggtcggtgg
    16321 tgctgttcgg gatgcattgt tagggctacc ggtcaaagac agagattggg tggtggtcgg
    16381 cagtacgcca caggagatgc tcgacgcggg ctaccagcag gtaggccgcg attttcctgt
    16441 gtttctgcat ccgcaaacgc atgaagagta tgcactggca cgtaccgaac ggaaatctgg
    16501 ttccggttac accggtttta cctgctatgc cgcaccggat gtctcgctgg aagatgatct
    16561 taagcgtcgc gatctgacca ttaatgcgct ggcccaggac gataacggcg agattatcga
    16621 cccgtacaac ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt cccccgcttt
    16681 tggcgaagat ccgttacgcg tgttgcgcgt agcgcgtttt gctgcgcgtt atgcccacct
    16741 cagtttccgt attgccgatg aaactctggc gctgatgcgc gagatgaccc atgcgggtga
    16801 actggaacac ctgacgcctg aacgggtatg gaaagagacg gaaagcgccc tcaccacccg
    16861 caatccccag gtgttcttcc aggtactgcg cgattgcggc gcactgcgcg ttttattccc
    16921 ggaaattgac tccctgtttg gcgttccggc ccctgccagg tggcacccgg aaatcgacac
    16981 gggtattcat accttaatga cgctctcaat ggcggcgatg ctgagtccgc aggtcgatgt
    17041 tcgtttcgca acgttatgcc acgatctcgg taaaggactg acgccgccag aactttggcc
    17101 acgtcatcat ggtcatggcc cggcgggtgt gaagttagtg gaacaactat gccagcgtct
    17161 gcgcgtgcca aatgaaattc gcgatttagc cagactggta gctgagtttc acgatctcat
    17221 ccacaccttt ccaatgctga acccgaaaac cattgttaaa ttgtttgatt ccattgacgc
    17281 ctggcgtaaa ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg acgtgcgcgg
    17341 cagaaccggt tttgaatcag cggactaccc gcaaggccgc tggttgcgcg aagcctggga
    17401 agtggcgcag tcagtgccga caaaagccgt cgttgaagca ggatttaaag gtgtggagat
    17461 tcgtgaggag ctgacccgac ggcggattgc ggcggtagcc agctggaagg aacaacgttg
    17521 cccaaagcct gattaacttt ttaaattcag tatattgcag aaatccctta ggtcggataa
    17581 gacgcacaag cgtcatatcc gacactatct ctcaatcaaa agaacacgac gtacaccgca
    17641 gccgccacaa taaagcgata aatggcgaac gggataaacg aaatgcgctt aatcaattgc
    17701 aggaaggttt taatcgctat cagcgccacc acaaaagcgg tgataaaccc aacggcaaac
    17761 atcgggatat cgccggtagt caggaagccc cagcttttgt agagatcgag cgccgttgcg
    17821 cccatcatca tcggcaccgc cagcaggaac gagaactcgg aagcagcgta acggctcacc
    17881 cccatcagca tcccacctga aatggtcgcc ccggaacggg agaaacccgg ccacagcgcc
    17941 agacactgga aacagccaat cataaacgcc tgacgatagg tcatatcatc aagacccggc
    18001 gcacgcggct ctttcggctt caggcattcg gcagcaatca gcaacagacc gccaacgacc
    18061 agtgcataca tgacatttat cgggttaaac aatgatttaa tcgtgtcgtg gaacaacagc
    18121 cccagtacca ccgccggaat catccccagc aaaatgtgga tcagcgttaa acgacctttg
    18181 ttttctcctt cgtgctgcaa cgggcggcca aagtggatgc caatcaggcc aaacagacgc
    18241 cgccagaaca tcactactac cgccagaatt gatcctaact ggatcacgac ttcaaaggtt
    18301 ttcgccgtgt cgccctcaaa ccccaacaag tgaccgacaa taatcatatg gcccgtgctg
    18361 gataccggca gaaattctgt caatccttcg accacaccca atattgccgc tatcagcagc
    18421 gagtgcatat cgctcatcaa taaaccccta aattataaaa atgtaacgct tgtccgaact
    18481 actgcagtat gaccaggtta taatcgtttg gtttaacagc tgtaaaatta attattttct
    18541 ttcagattat tgccacgctc aatgattacg ccaacattcg ccgcccgcgc cactgcgcct
    18601 ggcttgctga gtttgatacg cacccacggc gagttgaagc gtgctaacag cagttccgcc
    18661 acctcttcag ccacgcgttc caccagcgca aaacgcgccc cctcgacgtg gctgaccacc
    18721 gtttctgcaa tgtcagcgta actgaggcaa tccgccacat catcactttt cgccgcttta
    18781 cggttatccc acgccatttc gatatcgaac actaacttct gttcgatggt ctgttcccag
    18841 tcgtaaacac caatagtggt gattaccgaa agttgctcta taaatacaat atccatcacg
    18901 tcctgcctgc tttttggcta accggatacc acttccggcg aaatgtgcgt attatccaca
    18961 gattcatcgt tgaacacgaa ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg
    19021 aatgatcctc atcgcgtacc tctgcggctc catttccagt gccattctgg tttgccgctt
    19081 gtgtgggtta cccgatccgc gaaccagcgg ctccggcaat ccaggcgcaa ccaatgtgtt
    19141 acgtatcggt ggcaagggag cagccgtagc agtactgatt ttcgacgttc tgaaaggaat
    19201 gttgcccgtc tggggcgcgt atgaattagg tgtcagcccc ttctggctag gcttaattgc
    19261 catcgccgcc tgtcttggac acatctggcc cgttttcttc ggttttaaag gaggaaaagg
    19321 cgttgctacc gcttttggtg ccatcgcacc aattggctgg gatctcactg gagtaatggc
    19381 gggaacctgg ttactgaccg tgctattgag tggttactcg tcgctgggag cgattgtcag
    19441 tgcactgatt gctccgtttt atgtctggtg gtttaagcca caattcacct tcccggtttc
    19501 gatgctctct tgcctgatcc tgctgcgtca tcatgacaac atccagcgtc tgtggcgtcg
    19561 ccaggagaca aaaatctgga cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt
    19621 tctggtggat ctacatgacc tgatagcctt cgtcgggctt gcccagccgt tgctggcacc
    19681 atgccgccag aaattccaca cagacacgta atttcatgct gcgatagagc ggctcccggt
    19741 aaacagccca gatattggcg ctttgtgcat actccggcaa tactcgcacc agtttgccac
    19801 tctccagaaa cggcaacaca tcccactcgg aacgcagcat aatccctttg ccctccagcg
    19861 cccattgcaa cacaatctcg ccgctattgg aggaaagatg tccgcttacc ttcaccgatt
    19921 ttttctcctg accgttcccc aactcccata ttccgtgggt catatcgcgc tctttggtca
    19981 ccaggcagtc atgacgactt aattcctgta aggattgtgg ctgcggatat ttttgcagat
    20041 actcaggtgc tgcgcataat attcttttat tttttgtcaa aagatgcgca atataataat
    20101 caggaattgc gtcattaata cgaatatcca gatcaatatt atcctgcact aaatcaattt
    20161 gtcgatcgaa caattcaaaa tgcacctgta actcaggata attgcgcatc agttcggtaa
    20221 tagctggcgc aatatggctg cgcccaaaac caaaactgca accaatgcgg atcatcccct
    20281 ccgggcgcgt tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt
    20341 ccagcgcatg ttcgtaacaa cgttggccac tttcagtcag cgccacgccg cgagcggagc
    20401 ggttcagcag cgttgttgcc agcgtatttt caaggatttg gatccgcttg gtgacaaatg
    20461 caggcgtttg cccaagcgtc gccgctgcag cactaaaact cccgctatgg acgatttcca
    20521 ccagtacctg caagtctttg gctaaaggcc agctatttag cattgttaaa tatccgcagt
    20581 tttgataaac gcagtgtatt tcgcaaaaca ttaccattcc ctgctctggc gcaatattcg
    20641 ttcttatttt cgcgttgatt cacgaactgt taattacata ttcacggtgg caaaaaatat
    20701 aaaaccacat ttttagtggt agtttgtggc ggtgaatttt tccagacaaa tacataaact
    20761 ggagttgcca tgatgagcga aagtaataag caacaggcag tgaataagtt gacggagatt
    20821 gtcgctaact ttaccgccat gatttctacc cgaatgcctg atgacgtggt ggataaacta
    20881 aaacagctaa aggatgccga aacatcgtcg atgggaaaaa tcatctacca cacgatgttc
    20941 gataacatgc aaaaagcgat cgacctgaat cgtcctgcct gtcaggacac cggcgaaatc
    21001 atgttttttg ttaaggtcgg ttcccgtttc ccactgcttg gcgagctgca aagcatactc
    21061 aaacaagccg tggaagaggc aaccgtcaaa gcgccactgc gtcacaatgc ggtagaaatt
    21121 tttgacgaag taaacaccgg caaaaatacc ggcagcggcg taccgtgggt cacctgggat
    21181 atcgtccccg acggtgacga tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg
    21241 ctacctggcc gatcgaaagt gttaatgccg tcagaaggct acgaaggcgt agtgaaattc
    21301 gtcttcgaaa atatctccac cctcgccgta aacgcctgtc cgccggtact ggtgggcgtt
    21361 ggcatcgcta cctcggtgga aaccgccgca gtgctctcgc gaaaagccat tttgcgcccg
    21421 attggcagcc gccaccccaa tccgaaagcg gcagagctgg agctacgcct ggaagaagga
    21481 ctcaaccgtt tggggattgg tccacaaggg ctgactggca acagttcagt gatgggcgtg
    21541 catatcgaat ctgccgctcg ccatccgtca accatcggcg ttgctgtttc taccggctgc
    21601 tgggcgcatc gtcgcggcac actgctggtt catgccgatc tcacctttga aaatctgtct
    21661 cacacccgga gcgcgttatg aaaaagatcc tgacaacccc gatcaaagct gaagatctgc
    21721 aagatattcg cgtcggcgat gtgatctacc tgaccggtac gctggtgacc tgccgtgacg
    21781 tttgtcaccg tcgcttgatc gaactgaaac gtccgatccc ttacgatctc aacggcaaag
    21841 cgattttcca cgctggcccc atcgtgcgca aaaacggcga caaatgggag atggtatccg
    21901 tcggcccgac aaccagtatg cgtatggaaa gttttgaacg tgaatttatc gagcagaccg
    21961 gcgtgaaact ggtggtcggc aaaggcggaa tggggccgct gaccgaagaa ggctgccaga
    22021 aattcaaggc gctgcatgtg attttcccgg ctggctgtgc ggtactggcg gcaactcaag
    22081 tggaagagat tgaagaagtg cactggacag aactcgggat gccagagtcg ctgtgggtct
    22141 gccgggtcaa agagtttggc ccgctgattg tctctataga tacccacggc aacaacctga
    22201 tagccgaaaa taaaaagctg ttcgccgaac gccgcgatcc catcgtggaa gagatctgcg
    22261 agcacgtcca ctacatcaaa taaccctcac ggagaggctc gcccctctcc tttttcgcag
    22321 gcataacacg atgaaacctt ccactgaatg gtggcgatac cttgcgccgc tggcggtcat
    22381 cgccattatt gctctacttc cgcttcccgc agggctggag agtcatacct ggctctactt
    22441 tgccgttttt actggcgtga tcgttggact gatcctcgaa cccgtgccgg gtgccgtggt
    22501 ggcgatggtg ggtatatcca tcatcgccat tctttcgccg tggctgctgt ttagcccgga
    22561 gcagctcgcc cagccaggtt ttaagttcac cgccaaatcc ctctcctggg cggtttctgg
    22621 tttttctaat tcagttatct ggcttatttt cgccgccttt atgtttggta cgggctatga
    22681 aaaaaccggg cttggacgtc gcatcgcgct gattctggtg aaaaaaatgg ggcatcgcac
    22741 gctgtttctc ggctatgcag tgatgttctc cgagctgata ctcgcgcctg taacaccgtc
    22801 caactcggcg cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc caccgctcta
    22861 tcaatcacaa ccaaacgaca gcagttcgcg cagcattggc tcgtacatca tgtggatggg
    22921 gattgttgcc gactgcgtga ccagcgccat tttcctgacg gcgatggctc caaatttgct
    22981 gttaattgga ctgatgaaaa gcgcatctca cgccatgcta agctggggcg actggttcct
    23041 cgggatgttg ccgctcagca ttttactggt tttgctggtt ccctggctgg cttacgtgct
    23101 gtacccgccg gtactgaagt ctggcgatca ggtgccgcgc tgggcagaga cggaactgca
    23161 ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg taggcgcact
    23221 ggtgttgtgg atttttggcg gtgattatat cgatgccgcg atggtcggtt acagcgtggt
    23281 ggcgctgatg ctgcttctgc gcattatcag ttgggacgac attgtcagta ataaagcggc
    23341 gtggaacgtt ttcttctggc tggcctcgct tatcaccctc gctaccggac tcaacaacac
    23401 cggttttatt agctggtttg gcaaactgtt agcaggcagc ttaagcggtt attcgccaac
    23461 gatagtgatg gtggcgttga ttgtggtctt ttatctgctg cgctactttt ttgccagcgc
    23521 cacagcgtat acctccgctc tcgcaccgat gatgattgcc gccgcgctgg cgatgccgga
    23581 aatcccgctg ccggtgttct gcctgatggt tggtgcggca attggtctgg ggagcattct
    23641 tacaccatac gccaccggac ccagtccgat ttactacggc agtggttatc tgccaacggc
    23701 ggattactgg cgactggggg cgatttttgg gctgatattc ctcgtattgc tggtgattac
    23761 cggcttactg tggatgcccg tggtgttgct ttaagtgagg acggtgccgg aaattgctct
    23821 ccggcaccca atagttttac gcggccggta actctgccag tggccagcgc ggacgcacac
    23881 tcacgccgag atccgccgtc gcgcctgctt taaaccgcac cattccggca taggcgatca
    23941 tcgcaccgtt atccgtacaa aattccggac gcgcgtagaa cacttcgcca cggcgttttt
    24001 tcatcatttc cgccagtttc gcccgcaacg tgcggttagc actcacgccg cctgccatga
    24061 ccagtcgctt aaagcccgtc tgatccagtg ctcgcttaca cttaatcatc aacgtatcga
    24121 ccactgcatc ttcaaaggcg cgggcgatat cggcacgcgt ctgatcgtca gtgccgttgt
    24181 cacgtatggt atttgccgcg aaggttttca gaccagaaaa gctgaaatcc agccccggac
    24241 ggtcggtcat cggacgcggg aaaacaaagc gcccggcagt accctgagcc gccattttag
    24301 acagtaacgg tccgccagga taatccagcc ccagcagctt cgcggtttta tcaaacgctt
    24361 caccagcagc atcatcgata gactcgccga gcagctcgta ctgaccaatg ccagtcacgc
    24421 tgattaactg cgtatgaccg ccggaaacca gcagcgcgac aaacggaaat tccggtgggt
    24481 tatcttccag catcggcgct aacagatgtc cttccatatg atgtaccggg attgccggaa
    24541 cgtcccaggc aaacgccaga gaacgcccca cggtcgcgcc aaccagcagt gcgccgacta
    24601 atcctgggcc tgcggtatag gccacagcat caatgtcttt tgccgttaaa ccagactcct
    24661 ttagcgcctc ctggatcaac ggtacggttt tacgcacgtg atcgcgggag gccagttcag
    24721 gcacgacgcc gccgtagtca gcgtgcaatt tcacctgact atacaattgg ttggctaaca
    24781 aacctttttc atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt tcaataccca
    24841 gtacacgcat gacttgtttt acctcgcttt attaccgcgc agtgtaggac caatgcgggt
    24901 tgatgtaaaa ctttgttcgc ccctggagaa agcctcgtgt atactcctca cccttataaa
    24961 agtccctttc aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca gtaaaattcc
    25021 gcaccatttt gaaataagct ggcgttgatg ccagcggcaa accgaattaa tcaaaggtga
    25081 gaggcacatg ccggtaatta aagtacgtga aaacgagccg ttcgacgtag ctctgcgtcg
    25141 cttcaagcgt tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc gtgagttcta
    25201 tgaaaaaccg actaccgaac gtaagcgcgc taaagcttct gcagtgaaac gtcacgcgaa
    25261 gaaactggct cgcgaaaacg cacgccgcac tcgtctgtac taattccccg agagcgttgc
    25321 tctccgatca gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat gcgcggctta
    25381 ttttcgttta tgaattgcta aaaatcgggg cctatggctg gacgaatccc acgcgtattc
    25441 attaatgatc tgctggcacg cactgacatc gtcgatctga tcgatgcccg tgtgaagctg
    25501 aaaaagcagg gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa aaccccgtca
    25561 ttcaccgtta acggtgagaa acagttttac cactgctttg gatgtggcgc gcacggcaac
    25621 gcgatcgact tcctgatgaa ctacgacaag ctcgagttcg tcgaaacggt cgaagagctg
    25681 gcagcaatgc acaatcttga agtgccattt gaagcaggca gcggccccag ccagatcgag
    25741 cgccatcaga ggcaaacgct ttatcagttg atggacggtc tgaatacgtt ttaccaacaa
    25801 tctttacaac aacctgttgc catgtctgcg cgccagtatc tggaaaaacg cggattaagc
    25861 cacgaggtta tcgctcgctt tgcgattggt tttgcgcccc ccggctggga caacgtcctg
    25921 aagcggtttg gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg catgttggtc
    25981 actaacgatc aggggcgcag ttacgatcgt ttccgcgagc gggtgatgtt ccccattcgt
    26041 gataaacgcg gtcgggtgat tggttttggc ggacgcgtgc tgggcaacga tacccccaaa
    26101 tacctgaact cgccggaaac ggacattttc cataaaggcc gccagcttta cggtctttat
    26161 gaagcgcagc aggataacgc tgaacccaat cgtctgcttg tggtcgaagg ctatatggac
    26221 gtggtggcgc tggcgcaata cggcattaat tacgccgttg cgtcgttagg tacgtcaacc
    26281 accgccgatc acatacaact gttgttccgc gcgaccaaca atgtcatttg ctgttatgac
    26341 ggcgaccgtg caggccgcga tgccgcctgg cgagcgctgg aaacggcgtt gccttacatg
    26401 acagacggtc gtcagctacg ctttatgttt ttgccggatg gcgaagaccc tgacacgctg
    26461 gtacgaaaag aaggtaaaga agcgtttgaa gcgcggatgg agcaggcgat gccactctcc
    26521 gcatttctgt ttaacagtct gatgccgcaa gttgatctga gtacccctga cgggcgcgca
    26581 cgtttgagta cgctggcact gccactgata tcgcaagtgc cgggcgaaac gctgcgaata
    26641 tatcttcgtc aggaattagg caacaaatta ggcatacttg atgacagcca gcttgaacga
    26701 ttaatgccaa aagcggcaga gagcggcgtt tctcgccctg ttccgcagct aaaacgcacg
    26761 accatgcgta tacttatagg gttgctggtg caaaatccag aattagcgac gttggtcccg
    26821 ccgcttgaga atctggatga aaataagctc cctggacttg gcttattcag agaactggtc
    26881 aacacttgtc tctcccagcc aggtctgacc accgggcaac ttttagagca ctatcgtggt
    26941 acaaataatg ctgccaccct tgaaaaactg tcgatgtggg acgatatagc agataagaat
    27001 attgctgagc aaaccttcac cgactcactc aaccatatgt ttgattcgct gcttgaactg
    27061 cgccaggaag agttaatcgc tcgtgagcgc acgcatggtt taagcaacga agaacgcctg
    27121 gagctctgga cattaaacca ggagctggcg aaaaagtgat ttaacggctt aagtgccgaa
    27181 gagcgatcgg gaagcccccg acagccgcac tgagaggcag cggcaaatat ataagtacgc
    27241 cctcgtaatt atcgttggcg gtaaacaacc gttggatttc agcgttaacg gctgaaggac
    27301 atcgggtcaa tcgcccaaca ccaacctcat gaaataagtg tggataccgt cttatggagc
    27361 aaaacccgca gtcacagctg aaacttcttg tcacccgtgg taaggagcaa ggctatctga
    27421 cctatgccga ggtcaatgac catctgccgg aagatatcgt cgattcagat cagatcgaag
    27481 acatcatcca aatgatcaac gacatgggca ttcaggtgat ggaagaagca ccggatgccg
    27541 atgatctgat gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc gccgcgcagg
    27601 tgctttccag cgtggaatct gaaatcgggc gcacgactga cccggtacgc atgtacatgc
    27661 gtgaaatggg caccgttgaa ctgttgaccc gcgaaggcga aattgacatc gctaagcgta
    27721 ttgaagacgg gatcaaccag gttcaatgct ccgttgctga atatccggaa gcgatcacct
    27781 atctgctgga acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc gatctgatca
    27841 ccggctttgt tgacccgaac gcagaagaag atctggcacc taccgccact cacgtcggtt
    27901 ctgagctttc ccaggaagat ctggacgatg acgaagatga agacgaagaa gatggtgacg
    27961 acgataccgc cgatgatgac aacagcatcg acccggaact ggctcgcgaa aaatttgcgg
    28021 aactgcgcgc tcagtacatt gtaacgcgtg acaccatcaa agcgaaaggc cgcagtcacg
    28081 ctgccgctca ggaagagatc ctgaaactgt ctgaagtatt caaacagttc cgcctggtgc
    28141 cgaagcagtt tgactacctg gtcaacagca tgcgcgtcat gatggaccgc gttcgtacgc
    28201 aagaacgtct gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag aaaaacttca
    28261 ttaccctgtt taccggcaac gaaaccagcg atacctggtt caacgcggca attgcgatga
    28321 acaagccgtg gtcggaaaaa ctgcatgatg tctctgaaga agtgcatcgc gccctgcaga
    28381 aactgcagca gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa gatatcaacc
    28441 gtcgtatgtc catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg gttgaagcga
    28501 acttacgtct ggttatttct atcgccaaga aatacaccaa ccgtggcttg cagttccttg
    28561 acctgattca ggaaggtaac atcggcctga tgaaagcggt tgataaattc gaataccgcc
    28621 gtggttacaa gttctccacc tacgctacct ggtggatccg tcaggcgatc acccgctcta
    28681 tcgcggatca ggcgcgcacc atccgtattc cggtgcatat gattgagact atcaacaagc
    28741 tcaaccgtat ttctcgccag atgctgcaag agatgggccg tgaaccaacg ccggaagaac
    28801 tggctgaacg catgctgatg ccggaagaca agatccgcaa agtgctgaag atcgccaaag
    28861 agccaatctc catggaaacg ccgatcggtg atgatgaaga ttcgcatctg ggggatttca
    28921 tcgaggatac caccctcgag ctgccgctgg attctgcgac caccgaaagc ctgcgtgcgg
    28981 caacgcacga cgtgctggct ggcctgaccg cgcgtgaagc gaaagttctg cgtatgcgtt
    29041 tcggtatcga tatgaacacc gaccacacgc tggaagaagt gggtaaacag ttcgacgtta
    29101 cccgcgaacg tatccgtcag atcgaagcga aggcgctgcg caaactgcgt cacccgagcc
    29161 gttctgaagt gctgcgtagc ttcctggacg attaatcggt aggccggatc aggtgttacg
    29221 ccgcacccgg cactagtccc tccgcacaaa cgccaccttt tcggtggcgt ttttatcgcc
    29281 cacgcaccac cagcgcctgg tccagctcgc gatacgcttc aaccagtttc tccagtgaaa
    29341 cgcgacttaa accgctggga tttggcagca cccaaatctg cgtcgaacca atggtgagcg
    29401 tttgtttccc ccactgtgca ccgcgctggc tgaatccctg ttcatatgct tgtttgccca
    29461 gaatcgccaa cgcctgcggc tgataatctt caattttttc aatcagctta cgcccgcctg
    29521 cgtgtagctc ctgctttgaa acttcattgg cttgcaccgt tggacggtct accagtttgg
    29581 tgacgccaca acgataatcc agcagatgct gtgcctcctg cggcttcaac tgacggtcgg
    29641 taaacccggc ctgatatatc accttccaga agcgatttgc cggatgagca aagggaaaac
    29701 cagtcccggc ggatgaaaga ccagggttga taccgcaaaa cacgacccgt aaccctggag
    29761 ccaaaatatc ctcaaccata tttactcctt tctaacatca ccaggaaaag tataacggat
    29821 tgaaaataca ttgtttataa aaacagcagg cgcgcggtaa tggctggatt gcgacacgga
    29881 gttactttat aatccgctac catggcccct tagctcagtg gttagagcag gcgactcata
    29941 atcgcttggt cgctggttca agtccagcag gggccacc
//
LOCUS       DAOFSV010000047        29528 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.047, whole genome shotgun sequence.
ACCESSION   DAOFSV010000047 DAOFSV010000000
VERSION     DAOFSV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.047"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(1..218)
                     /locus_tag="QBH27_002195"
                     /pseudo
     CDS             complement(1..218)
                     /locus_tag="QBH27_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            225..826
                     /locus_tag="QBH27_002196"
                     /pseudo
     CDS             225..826
                     /locus_tag="QBH27_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(842..918)
                     /locus_tag="QBH27_002197"
     tRNA            complement(842..918)
                     /locus_tag="QBH27_002197"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(882..884),aa:Arg,seq:tct)
     gene            1189..2055
                     /gene="folD"
                     /locus_tag="QBH27_002198"
     CDS             1189..2055
                     /gene="folD"
                     /locus_tag="QBH27_002198"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDQ7014504.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            2057..2269
                     /gene="ybcJ"
                     /locus_tag="QBH27_002199"
     CDS             2057..2269
                     /gene="ybcJ"
                     /locus_tag="QBH27_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDQ7014505.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            2377..2898
                     /locus_tag="QBH27_002200"
     CDS             2377..2898
                     /locus_tag="QBH27_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ7014506.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKWVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2934..4319)
                     /gene="cysS"
                     /locus_tag="QBH27_002201"
     CDS             complement(2934..4319)
                     /gene="cysS"
                     /locus_tag="QBH27_002201"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDQ7014507.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            4493..4987
                     /gene="ppiB"
                     /locus_tag="QBH27_002202"
     CDS             4493..4987
                     /gene="ppiB"
                     /locus_tag="QBH27_002202"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDQ7014508.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4990..5712
                     /gene="lpxH"
                     /locus_tag="QBH27_002203"
     CDS             4990..5712
                     /gene="lpxH"
                     /locus_tag="QBH27_002203"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDQ7014509.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5823..6152
                     /locus_tag="QBH27_002204"
     CDS             5823..6152
                     /locus_tag="QBH27_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014510.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERYILIVGICFLPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            6248..6757
                     /gene="purE"
                     /locus_tag="QBH27_002205"
     CDS             6248..6757
                     /gene="purE"
                     /locus_tag="QBH27_002205"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDQ7014511.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            6754..7821
                     /gene="purK"
                     /locus_tag="QBH27_002206"
     CDS             6754..7821
                     /gene="purK"
                     /locus_tag="QBH27_002206"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDQ7014512.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSHLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(8355..9248)
                     /locus_tag="QBH27_002207"
     CDS             complement(8355..9248)
                     /locus_tag="QBH27_002207"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDQ7014513.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9245..10060)
                     /locus_tag="QBH27_002208"
     CDS             complement(9245..10060)
                     /locus_tag="QBH27_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDQ7014514.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(10071..11330)
                     /locus_tag="QBH27_002209"
     CDS             complement(10071..11330)
                     /locus_tag="QBH27_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDQ7014515.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIACLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(11340..13007)
                     /locus_tag="QBH27_002210"
     CDS             complement(11340..13007)
                     /locus_tag="QBH27_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDQ7014516.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEKALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            13324..14373
                     /gene="allD"
                     /locus_tag="QBH27_002211"
     CDS             13324..14373
                     /gene="allD"
                     /locus_tag="QBH27_002211"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDQ7014517.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            14395..15630
                     /gene="allC"
                     /locus_tag="QBH27_002212"
     CDS             14395..15630
                     /gene="allC"
                     /locus_tag="QBH27_002212"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDQ7014518.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            15641..16426
                     /gene="allE"
                     /locus_tag="QBH27_002213"
     CDS             15641..16426
                     /gene="allE"
                     /locus_tag="QBH27_002213"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDQ7014519.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFVN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVYGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(16555..17700)
                     /gene="glxK"
                     /locus_tag="QBH27_002214"
     CDS             complement(16555..17700)
                     /gene="glxK"
                     /locus_tag="QBH27_002214"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDQ7014520.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVVPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(17722..19023)
                     /locus_tag="QBH27_002215"
     CDS             complement(17722..19023)
                     /locus_tag="QBH27_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDQ7014521.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IAAPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYSRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(19079..20440)
                     /gene="allB"
                     /locus_tag="QBH27_002216"
     CDS             complement(19079..20440)
                     /gene="allB"
                     /locus_tag="QBH27_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDQ7014522.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(20500..21954)
                     /locus_tag="QBH27_002217"
     CDS             complement(20500..21954)
                     /locus_tag="QBH27_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDQ7014523.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(22124..23002)
                     /gene="glxR"
                     /locus_tag="QBH27_002218"
     CDS             complement(22124..23002)
                     /gene="glxR"
                     /locus_tag="QBH27_002218"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDQ7014524.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(23102..23878)
                     /gene="hyi"
                     /locus_tag="QBH27_002219"
     CDS             complement(23102..23878)
                     /gene="hyi"
                     /locus_tag="QBH27_002219"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDQ7014525.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(23891..25672)
                     /gene="gcl"
                     /locus_tag="QBH27_002220"
     CDS             complement(23891..25672)
                     /gene="gcl"
                     /locus_tag="QBH27_002220"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDQ7014526.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(25762..26577)
                     /gene="allR"
                     /locus_tag="QBH27_002221"
     CDS             complement(25762..26577)
                     /gene="allR"
                     /locus_tag="QBH27_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDQ7014527.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDNQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(26655..27137)
                     /gene="allA"
                     /locus_tag="QBH27_002222"
     CDS             complement(26655..27137)
                     /gene="allA"
                     /locus_tag="QBH27_002222"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDQ7014528.1"
                     /translation="MKLQVLPLSQKAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            27367..28293
                     /gene="allS"
                     /locus_tag="QBH27_002223"
     CDS             27367..28293
                     /gene="allS"
                     /locus_tag="QBH27_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDQ7014529.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            28362..29468
                     /gene="mnmH"
                     /locus_tag="QBH27_002224"
     CDS             28362..29468
                     /gene="mnmH"
                     /locus_tag="QBH27_002224"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDQ7014530.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTYACGY"
BASE COUNT         7310 a         7975 c         7365 g         6878 t
ORIGIN      
        1 cctgccgggg ataaccaggt gacattttcc ggtaacggac cgagttcaga aataaataac
       61 gcgtcccctg atgccacgtc ataaaccgtt ttacccagat ggtcttcaac gagatgccac
      121 gatgcctcat cactgttgaa aacaaccacg aagccagccg gaatatctgg cggtgcaata
      181 tcggtactgt ttgcaggcaa acctgtatga ggtggaatgg cacaagagac ttacaaaaga
      241 atcaggcaaa acgcctgatt gatgagagcc ccgaactact gaaatctgtc gtcaaatttg
      301 cgctggtaac tgttctgaga aagtcgaaca tcataaatct ggagtggcaa cagattgaca
      361 tgcagcgaca agttgccagg gtggatccag aagatagcaa atcaaatcgc gccattggtg
      421 tggcgctgaa cgataccgcc tctaaagtgt tgcgtgatca aacaggcaag catcacaaat
      481 gtgggtgtac ataccagggc ggctaagcga gcagatggaa catcaatgcc tgcgctcagg
      541 aagatgcgca ttgacagcaa gacagcatgg ctatcagctt gtcgtcgtgc aggagttgaa
      601 aatttccgtt tccatgacct ctggcatacc cgggcaagca ggttgattcc gtcaggcgtc
      661 ccattatcag tgcttcagga aatgggcgga tgggagtcca gagaaatggt tcgtagatat
      721 gctcaccttg cgcctaatca tttgactgag cacgcgagga aaatagacga catttttggt
      781 gataatgtcc cactctggaa ttatagaaga aataaagaag gcgtaactga ttgaatttta
      841 atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg ttgctctatc
      901 caactgagct aagggcgcat tgataccacg gtgtgctgta atcgcgtgaa ttatacggtc
      961 aacccttgct gagtcaatgg cttttgaacc agttgctgaa caagtgtacg accgcatcag
     1021 atgtagctca ctatggcggc aaaccaacga cggttatcat gataggtgtg aatcagccag
     1081 gtacagaagg ttttactttt cagaaaacga aaactgacag cgcgccacgc ttctgacaaa
     1141 ataggcgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat ggcagcaaag
     1201 attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca aaaagttcag
     1261 gcgcgtattg cagccggact gcgggcgcca ggactggccg ttgtgctggt gggtagcaac
     1321 cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt cgggttcgtc
     1381 tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga gcttatcgat
     1441 gcgctgaatg ccgacaacac cattgatggc attctggttc aactgccgtt accggcgggt
     1501 attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga cggtttccat
     1561 ccttacaacg tcggtcgtct gtgccagcgc gcgccacgtc tgcgtccctg caccccgcgc
     1621 ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggccttaa cgccgtggtg
     1681 attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgctgct ggcaggttgc
     1741 accaccaccg tgactcaccg tttcactaaa aatctgcgtc accacgtaga aaatgccgat
     1801 ttgttgatcg ttgccgtcgg caagccgggc tttattcctg gtgactggat caaagaaggc
     1861 gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt gggcgacgtc
     1921 gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttccggg cggcgttggc
     1981 ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga atatcatgat
     2041 ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg ttgagctgtg
     2101 cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa tcgcgatagc
     2161 cgaaggcctg gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca aaatcgtcgc
     2221 cggtcagaca gtgagttttg caggtcacag cgtacaggtt gttgcctgat ccctttcccg
     2281 gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc cgttcatcca
     2341 tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat tacgcacgcc
     2401 gccgttcccc tatgcattgg tttagggttg ggttcgaaag ccatccctcc gcgtctgtta
     2461 tttgccggaa tcatcctggc gatgctgccc gatgccgatg tattgtcgtt taaatttggc
     2521 gttgcttacg gaaatgtttt tggtcatcgc gggtttaccc attcgctggt gtttgcgttt
     2581 gttgtcccgc tgttatgtgt gctgattgga cgtcgatggt tcagggctgg gctgattcgc
     2641 tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct tgctggattc ggtaaccact
     2701 ggcggtaaat gggttggctg gctgtggccg tggtcagatg aacgcttttt cgctccctgg
     2761 caggtgatta aagtcgcgcc gtttgcattg tctcgttaca ccacgccgta cgggcatcag
     2821 gtgattattt ctgaattgat gtgggtgtgg ttgccgggga tgttactgat gggaatgttg
     2881 tggtggcgca gacgataacc ggatgcgaaa actcgcatcc ggcaatagcg caattactta
     2941 cgacgccagg tggtcccttg cgggccatct tccagcacga tccccatctc gttaagacgg
     3001 tcacgcgccg catctgccgc cgcccagtct ttcgctttac gggcatccag acgctgttga
     3061 attaacgctt caatctcagc cacttcgctg tcgtctgcct gcgcaccgct ttgcaggaac
     3121 gcttccggtt cttgctccag caggcccaat acggcagaaa gtttacgcag gtgagacgcc
     3181 attgcattcg ccgctgccat atcttctgct ttcagacggt tcacttcacg cgccatatca
     3241 aacagtacgg aataggcttc cggagtgttg aaatcgtcgt tcatcgcctc gataaagcgc
     3301 gcttcaaacg cttcgccacc ggcaggcgca acggttttgt ctgtgccgcg cagcgcagtg
     3361 tagagacgtt ccagcgccgc acgcgcctgt ttcaggttct cttcgctgta gttcaactga
     3421 ctgcgatagt gacccgacat caggaagtaa cgcacagttt ccgcatcgta gtatttcagc
     3481 acatcacgca cggtgaagaa gttacccagc gatttagaca tcttctcgcg gtcaaccatc
     3541 accataccgg agtgcatcca gtaattcaca tactggccat cgtgcgcaca ggtggactgg
     3601 gcaatttcgt tctcgtggtg cgggaacatc aggtcagaac cgccgccgtg gatatcaaag
     3661 tggttaccca gctgcttgca gttcattgcc gaacattcaa tgtgccagcc aggacggccc
     3721 gcgccccacg gagacggcca gctaggttcg ccctctttcg acattttcca cagaacgaag
     3781 tccatcgggt tgcgtttgtc gtcgaccacg tcaacgcgcg cgcctgcctg cagctgatcg
     3841 agatcctgac gcgacagcac gccatatgtc ggatcggtcg ggacgtcgaa catcacgtcg
     3901 ccgttgtcag ccacataagc gtgaccttta gcgatcagtt gttcagtgag ttcaataatt
     3961 tcggcgatat ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt caaagcatca
     4021 aaatctttgt gcatttcggc gatcatgcga tccaccagcg ccacaaagct ttcgccattt
     4081 tcattggcgc gtttgatgat tttgtcgtcg atatcagtaa tgttgcgcac atacttcagc
     4141 ttatagccga ggaaacgcag atagcgcgca accacgtcaa aggcaacaaa ggtacgcccg
     4201 tgaccgatat gacagagatc gtaaacggtg attccacaca cgtacatgcc gacttccccg
     4261 gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag tattgaagat ttttagcatc
     4321 gaagattccg tttagacatg tgtgggtaat tgagttgcgt ataataccca tatttcccgc
     4381 gcgaatcagc atacattgcg agatgatccg atcgtgcggt tatgctataa caccacccta
     4441 tatatgaccc gaaccgggtt gaagcaccaa tcaaacggaa caggatgcaa aaatggttac
     4501 tttccacacc aatcacggcg atattgtcat caaaactttt gacgataaag cacctgaaac
     4561 agttaaaaac ttcctggact actgccgcga aggtttttac aacaacacca ttttccaccg
     4621 tgttatcaac ggctttatga ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc
     4681 caccaaagac ccgatcaaaa acgaagcgaa caacggtctg aaaaataccc gtggtacgct
     4741 ggcaatggcc cgtactcagg caccgcactc tgcaactgcc cagttcttca tcaacgtggt
     4801 tgataacgac ttcctgaact tctctggcga aagcctgcaa ggttggggct actgcgtatt
     4861 tgctgaagtg gttgaaggca tggacgtggt agacaaaatc aaaggtgtgg caaccggtcg
     4921 tagcggtatg caccaggacg tgccaaaaga agacgttatc attgaaagcg tgaccgttag
     4981 cgagtaatcg tggcgacact ctttattgca gatcttcatc tctgcgtgga agaaccggcg
     5041 atcaccgccg gttttctgcg ttttttagca ggggaggccc gcaaggccga tgcgctgtat
     5101 attcttggcg atctgtttga agcctggatc ggcgacgacg atcccaaccc acttcatcac
     5161 cagatggccg cagcgatcaa agcggtatcc gattccggcg ttccctgtta tttcattcat
     5221 ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg aaagcggcat gacgttattg
     5281 ccggaagaaa aggtgctcga actttatggt cgtcgggtgt tgattatgca tggcgacacg
     5341 ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca aagtccacaa accctggctg
     5401 caaacgctat tcctcgccct gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcgc
     5461 gcgaacagta aagaagccaa cagcagtaaa tcgctggcga tcatggacgt taaccaaaac
     5521 gcggtggtca gtgcgatgga aaaacatcaa gtgcaatggc tgatccacgg gcatacccat
     5581 cgcccggcgg tgcatgaact tatcgccaat cagcaacctg cttttcgcgt ggtactgggt
     5641 gcctggcata cagaaggttc gatggtgaaa gtcacggcgg atgacgttga gctaatccat
     5701 tttcctttct gaccacgaat ggacagattc tgaaaaagca ccgaactaac gcaacctgta
     5761 tatctttttt aaaatcagca aggtaataat ctctgagtga aataacaaca gggaatatta
     5821 ttatgcaacg ctgcaacgga tttttgattt ctggcatcgc ccttacggca ggagcgccac
     5881 tgtgtattag ctggttcggt cttgtcggct tctattttgg cgcattgtca ctggtagctg
     5941 cggcggtcat ttcctggata gtctatttct ggctcattta tttagtactc acccgccccc
     6001 ataaccaacg ctttgtagaa agatatatat tgattgtcgg tatatgcttt ttaccgtgga
     6061 gcattgtgct gatatgggca ttctttgcca gttcagccag agcgcataat caacacgcag
     6121 aacaaattaa aaactcaaca actgacggtt aattccgcct acgcaaccgt tttcctagca
     6181 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     6241 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     6301 cgactgggct accatgcagt tcgccgccga aatcttcgaa atcctgaatg tcccgcacca
     6361 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     6421 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgcctgg
     6481 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     6541 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     6601 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcgtta ctggcagcac aaattcttgc
     6661 gactcatgat aaagaactgc accagcgtct gaatgactgg cgcaaagcac aaactgatga
     6721 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     6781 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     6841 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     6901 atcgaacgct ggccggaaac tgcattaacc cgcgagctgg cgcgccagcc ggcctttgtg
     6961 aaccgcgatg tgttcccgat tattgccgac cgtctgactc agaagcagct tttcgataag
     7021 ctccacctgc cgaccgcacc gtggcagtta cttgccgaac gcagcgagtg gcctgcggtg
     7081 tttgatcgtt taggtgagct ggcgattgtt aagcgccgca ctggtggcta tgacggacgc
     7141 ggtcaatggc gtttacgcgc cgctgaaacc gaacagttac cggctgaatg ctacggcgaa
     7201 tgtattgtcg agcagggcat aaacttctca ggtgaagtgt cgctggttgg cgcgcgcggt
     7261 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggcattttg
     7321 cgcaccagcg tcgctttccc gcaggccaac gcacagctgc aggcgcaagc tgaagagatg
     7381 ttgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     7441 acgccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag tgggcactgg
     7501 acgcaaaacg gtgccagcat cagccagttt gagctacatc tgcgggcaat taccgatctg
     7561 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     7621 ctgaattatg actggctgaa actgccgctt gtgcatctgc actggtacga caaagaagtc
     7681 cgtccgggcc gtaaagtggg gcatttaaat ttgaccgaca gcgacacatc gcatctgacc
     7741 gcgacgctgg aagccttaat cccgctgctg ccaccggagt atgccagcgg cgtgatgtgg
     7801 gcgcagagta agttcagtta acctggtgct catttgattg ccggatgcgg cgtgaacgcc
     7861 ttatccggcc taccgttcca acaccgcctg tagacctgat aagacgcacc agcatcgcat
     7921 caggcatggt gtgccgattg ccggatgcgg cgtgaacgcc ttatccggcc taccgttccg
     7981 acaccgcctg tagacctgat aagacgcgcc agcatcgcat caggcatggt gtgccgattg
     8041 ccggatgcgg cgtgaacgcc ttatccggcc taccgttcca acaccgcctg tagacctgat
     8101 aagatgcgcc agcatcgcat caggcatggt gtgccgattg ccggatgcgg cgtgaacgcc
     8161 ttatccggcc taccgttccg acaccgactg taggcctgat aagacgcgcc agcatcgcat
     8221 caggcatggt gtgccgattg ccggatgcgg cgtgaacgcc ttatccggcc taccgttccg
     8281 acaccgcctg tagacctgat aagacgcgcc agcatcgcat caggctagat gtgccaattg
     8341 cctggtgccc atcactacag cgaaatacaa gtccccgctt cgcccgccag cgtctcttca
     8401 atccgcgata acgccccaat ccacgcgggt ttaccacggc ttctgacata gccactcacc
     8461 gccgttacct tcggccccat cgaaccatcg gctttggcaa atggcgctaa ctcatccggt
     8521 gtggcatggc gaatggcacg ttgctgcggc gttccccagt tttcatatac cgcatcagca
     8581 tcggtgagga tcaccagtcc atcggcatta atctgctcgg cgagcaacgc agcggcgaga
     8641 tctttatcaa tcaccgcttc actccctgcc ccgtcttccg tcacaggcac accaccgccg
     8701 ccactgcaaa tcaccacatg cccctctttg agcaacaact cgatggcttc gctgtcgaga
     8761 attttacgcg gttgcggaga cgccaccacc cgacgtaaat atttaccatc acgtttcatc
     8821 tgccagccgt aagccgcttc cagtgcctct tgttcttctg gctgataaac cggaccgata
     8881 aacttctctg gctgtaaaaa cgccggatca tcaggcgaaa cctcaatgcg cgtcagcacc
     8941 gtcgtgacag gcggcatctg cggctgtgcg ctcaagctct gcgccagcat atagccaatc
     9001 atcccctggc tttccgccac cagtacatcc agcggatacg gttctacctc tttccacgcc
     9061 agattctgta acgccagcag ccccacctgc ggtccgttgc cgtgaacaat tgccagccga
     9121 taagaacggg ccaggcgcgc cagcgcgggt acagcactgg caatattgcg atattgattt
     9181 tttgccgtca gcgcctcacc gcgctggagt aaggcgttgc ccccaagagc cacaaccagt
     9241 gttttcatgg tttcccttgt aataattgtt ggccaagcca gaaccccagc agcgtatctg
     9301 cccctgacgt atgccccagc gccagcagcg aatcgatcgc cgctgcggta cgtttcgggc
     9361 aactcagcgc atgaacaaag tgcaggagtg gcgaggcgaa atatccttgc gcggcataac
     9421 gtaaataact gacgctcacc gcagtggtaa cgagttgaag attgtcggaa caggcaaaaa
     9481 acgggcgacc ggagcgcgca tctaaagcgc cataatacca ggccgccagc agcattccgc
     9541 tcagcgtgtc atcatgactt ggcgttaatc cggggccttt acccagccag tgccgccagt
     9601 cggtcttaac gccattgagc gcggcctgaa aacagtgacg aaactggcgt aattcagcag
     9661 gcagcggatc gcttgccgcc aatgccagtg gcccgaaaag cccggtttcc tccgcgcgtt
     9721 gcatccatgc aacggcaagc ggttgaggat gcgcaggcgg cgtaatacgt agcaaacaat
     9781 aacgctgtgg ctgtttaacc gtgaaacgcc cgagacgaat accttgtgcc acaacctgtg
     9841 gtcgttcatt gccgcataat ccaccgcata acgcatcgaa ttgcgcgcgg cgaagcaccc
     9901 atccgccggg gccgaaacca ctcccctgac gatgcagcgt taacagttcg ccgctttccg
     9961 tcatcaggtt aatcgcccgc cgccacactc ccgctaaccg ccaggactgg cgataattcg
    10021 gtgcgctacg gctggcaagc agaggatgga tgatcgtcat tgtgcgttcc tcaaccaatc
    10081 cccatgcttt cagccagtgc ttccagcgcc tgttcaaagc acgccagcgg tgcccgcacg
    10141 gtgcctgcgc caatctgccc gatccccgcc tctttatggg cgataccggt attgatgagt
    10201 ggcgtaatgc cggtttctac cacgcgacga atgtccagcc ccaggcacgc cccctggaaa
    10261 tcccagccgg ggatctgcaa ctgcatattg cgttcaaggt aaatttccgc catctcttca
    10321 gataccgctc tggccgcttc catgccaccc gcaccgacaa agcgcgttac gccaggcgct
    10381 gcgatcattg ccgcacctcc gataccaaag gtttcggtaa tcgcgctatc gcccatatcc
    10441 gggttcgcct gctcctgcga gaagccggtg aaaaacagac cttgcggagt gtttacaggc
    10501 gcagtaaacc agcgttcacc cagcccgctt acccgaatcc cgaacatatt gccgttgcgg
    10561 gtcatagccg taacgatgct gcctgcgcgg atcatcgcgc ctgcatccat cgccgccttg
    10621 cagtaggcca tcgcgaggtt gaggaagaac tgatcggtca cgctgagaaa atccatcact
    10681 tccgcaatgt gctgtttatc atgatcgagg caagcaattt gtggggccag cgtacgcatt
    10741 aacagtgctg aggaagcaat attgcgttga tggaactcat cgcccatggt aatgccctgc
    10801 gccatcattg ccgtgagatc gataccgcgc tccatgcgcc ccagcgccgc gcttaatact
    10861 ggcattaaca catcgcgcat ccagcgatgg cgagtcagga catcttcgcc gtaagcgcca
    10921 aaacgcatca ctttgccgat accttcgttg aggttgcagt acgcccggtt gccgtcggtc
    10981 acgttctcga ccaccagcat cggcatactg gcagaagtaa tgccgcccat tggcccgacg
    11041 gcattcacat ggtgacaagg aatgaagttc acttccccct gctccagtat tgccagcgcc
    11101 tgcgcttcat ctttcgccca gccttcgaac agacatgcgc ccacgcacgc ccctttcatg
    11161 ggtccggtca tctcctgcca gcgcattggc ggcccggcgt gaagcagtgt tttgcccttg
    11221 tttagttcgc tgataagtga agaagccggt tgcacatcca gccagtgtgg acgagcacga
    11281 cgaatttgtt cgataaccgc agcattggct tgcgccactg atgtaaacat gggaacccct
    11341 tattgcaaac gttctaataa acgagccagt tttttattgc cgccggcgac tggcgaccat
    11401 tggtaatgca caaccggttt gctggcgctt tgtagctcca gcgcaaagct gcgtaatccg
    11461 atgttaatca cggcgacgtt ttccagtaat gacggtgtgt gttgctgtgt ggcaggcgag
    11521 agcggatgaa ttaacgccgc agccagcaag gtggcttccg gtagcgaact cactaccgcg
    11581 atccccgcat cttccagcgt ggcgatttgc tgcgagcggc attgcgggtc acgttcagtg
    11641 cctgtcaccg tggcaatggc atacagtggt tgattgtctg aacgcgcggc acaggctttt
    11701 tgccaggcgc tcaccagcga ggcggcagga tcggcggtcg cgccgaagcc aatcacgaca
    11761 tcaagcagca acactcgcac ttgcggttta gcgccgagat cggcaattaa caggttgcgt
    11821 aaggtcgggt cgatcatcgg atggggacgc ccgacggtgt aaaaatcatc gcccaggtcg
    11881 aggatctgat gtccgtcggc gtccagcatc atgccatgtt gatgggtatc gtcggcttcc
    11941 acgccaaggt gtccggcaag taatcccgcc gcttcagcag ccagcgtacc gccggtatac
    12001 aaaccgcaaa taaatccgct gctgacaggc gctatcgcgt tacgtcgcgc agtgacgcgt
    12061 gaaagcagac aagccagacg agcggcctca tccagcgagg aggcaaacca gacattctcg
    12121 tcgcgggcca ccgccggggt ataacctaaa aacagcgcca ccgtcggttt gccagttgct
    12181 ttcatggcat taacaatttt cagacgcaca gtttcggcag gtggttttga aacaaatgcc
    12241 agcacttcgc ttttctcgtc tgcactgagc atttccagcg ccgttagcgc actgatgccg
    12301 cccacttcac ggctgagatc gcgcccgcca agaccaatcg cgtgagtaat tccctcccct
    12361 gccagcgcaa tctgtgagca cagctcctga atcccggtac cggaagcgcc tatgacgcca
    12421 atgttgcctt ccggcatcac attagcaaaa gccagcggcg tggcagcaat catcgacgta
    12481 ccgcagtctg gccccatcac cagcaagcct ttttcccgcg cgcgggtttt aagttggatt
    12541 tcatcttcca gcgtgacgtt atcggagaac atcatcacgt tgaggttgcg atccagcgcc
    12601 tggttcgcca actccgccgc atactcacca gccactgaaa tcagcgccag attggcatcg
    12661 ggtaatttct gactggcact gtcccagcga cgcacctgcg tcaacgcctg gctgctgcct
    12721 gaaccctgcg ccagttgttt tagcgccttt tcaagctgct gcataaccgc ctgcgcgatc
    12781 cccgcatccg ccgcttcgct acgaattgcc acgcaaatat cgttcggcgt ggcgttatta
    12841 aaatcgtcat gccagaaacc tgtagtatct aataacgctt tattggcggg cgtacccatc
    12901 attacggaaa catcatcaac attttctgat tcgctgagtt ttcgtgaaat aatcattaaa
    12961 ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt ggatcataac aactccttaa
    13021 aaattgcacc catggcaatt gcacgagcag tgttaatgtg gcgattacgg tatcgatgac
    13081 aacgagtaaa taaggtgatg aatatcacac ggggattatt aacaaaagaa ttcaaataac
    13141 cgccagacta ttacgcgtta ttttttaata agcatggttg ttttaaaaac caggttaaaa
    13201 gcacgaagat tatcaaataa attctaacta tatttttttg cctgtctgga tcacataatc
    13261 cagatatttt ccctgtatgt taattgcagt catgcttcac accgtcgtta aaaaggaaga
    13321 cagatgaaaa tcagtcggga aacactccac cagctaatcg agaataaact ctgccaggct
    13381 gggttaaaac gtgagcacgc tgcaaccgtg gctgaagtat tggtttacgc cgatgccaga
    13441 gggatccact ctcatggcgc ggtgcgcgtt gaatactacg ccgaacgcat ttcaaaaggc
    13501 ggcaccaacc gcgaaccgga gtttcgtctt gaggaaaccg gaccgtgctc ggcaatttta
    13561 catgccgaca atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca tgccatcaaa
    13621 accgcccagc aaaaaggcgt tgcggtggtc ggtatcagcc ggatgggtca cagcggcgca
    13681 atctcttatt ttgtgcagca ggctgcccgc gccggattga ttggcatttc gctgtgccag
    13741 tccgatccaa tggtggtgcc gtttggcggc gcggaaattt actacggcac taacccactg
    13801 gcctttgccg cgccgggaga aggcgacgag atccttacct ttgatatggc gactaccgta
    13861 caggcatggg gaaaagtact cgacgcccgc tcgcgcaata tgtctatccc ggatacctgg
    13921 gcagtcgata aaaacggtgc accaacaacc gatccgttcg cggtacatgc tctgctcccc
    13981 gccgctgggc caaaagggta tggcctgatg atgatgattg acgtcctctc aggcgtctta
    14041 ctcggcttac cgttcgggcg acaggttagt tcgatgtatg acgatttaca cgccgggcgt
    14101 aatttggggc aattacatat agttattaac ccgaactttt tctcctccag cgaattattc
    14161 cgtcaacatc ttagccagac catgcgcgaa ttaaatacca ttacccccgc gcccggtttt
    14221 aatcaggttt attatcccgg acaggatcag gatattaaac aacgcaaagc cgccgtcgaa
    14281 ggcatcgaaa ttgttgatga tatttaccag tatttaattt ccgacgcgct ttataacacg
    14341 tcatacgaaa cgaaaaatcc ctttgcgcaa taattattga gacaggactt tcttatgatt
    14401 acacatttcc gtcaagctat agaagaaacg ctgccctggc tttcctcttt tggcgctgac
    14461 ccagcgggtg ggatgacccg tttactttat tcgccggaat ggctggaaac ccagcagcag
    14521 tttaaaaaaa gaatggcagc aagcgggctg gaaacacgtt tcgatgaagt ggggaattta
    14581 tacggtcgcc tgaatggcac cgaatatcca caggaagtgg ttctgagcgg ttcgcatatc
    14641 gataccgtgg ttaacggcgg taacctcgac gggcaattcg gtgcgctggc ggcgtggctg
    14701 gcaattgact ggctgaaaac gcaatacggc gcgccgttac gtacggtcga agtggtggcg
    14761 atggcagaag aagaaggcag ccgcttcccg tatgttttct ggggcagtaa aaatattttt
    14821 gggctggcga atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa tagttttgtc
    14881 gatgcgatga aggcttgcgg atttactctg ccgaacgccc cactaactcc gcgtcaggat
    14941 attaaagcct ttgtcgaact gcatatcgaa cagggctgtg tgctggaaag taatgggcag
    15001 tcaattggcg tggtgaatgc aattgtcggg caacgtcgct atacggtgac gctgaacggt
    15061 gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc gtgatacggt ttacgctttc
    15121 agtcgcattt gccatcagtc tgtcgaaaaa gcgaaaaaga tgggcgaccc gctggttctg
    15181 acttttggca aagtagagcc gcgcccgaat acggtgaatg tagtgccggg taaaaccacg
    15241 ttcaccattg attgtcgtca taccgatgct accgtgctgc gcgatttcac ccaacagtta
    15301 gaaaacgaca tgcgggcgat ttgcgatgaa atggatattg gtattgatat cgatttatgg
    15361 atggacgaag aacccgtgcc gatgaataag gagctggtcg ccaccctgac agaattgtgt
    15421 gaaagcgaaa aactgaatta ccgggtgatg cacagtggtg ccgggcacga cgcgcaaatt
    15481 ttcgcgcctc gcgtgccgac ctgcatgatt ttcattccca gcatcaacgg gatcagccat
    15541 aacccggcgg aacgcaccaa tattaccgac cttgccgaag gggtcaaaac gttggcactc
    15601 atgctttatc aacttgcctg gcagaaataa ggagttataa atgggatatt taaataacgt
    15661 caccggttac cgcgatgatt tactggctaa ccgtgcgatt gttaaacacg gtaatttcgc
    15721 actgttaaca ccagacggtc tggtaaaaaa tattattccg ggctttgtaa attgtgacgc
    15781 gacaatcctc tccacgccaa agctgggtgc ctcttttgtt gattatctgg tcacactgca
    15841 tcaaaacggc ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt
    15901 gatctctgga aatatcactg ccaaagccga agggaaaaca tttgccttaa gcgaaggtgg
    15961 ctatctttat tgcccgccag gctccttaat gacgtttgtt aacgcccagg ccgaagacag
    16021 ccaaatcttt ttatataagc gtcgctatat tccggtagaa ggtcatgcgc cgtggctggt
    16081 ttatggcaat gccagcgaac tggaacgcat tcattatgaa ggcatggacg atgttattct
    16141 gctggatttt ctgcccaaag agttaggttt tgatatgaac atgcatatcc tctcttttgc
    16201 accaggtgcc agccacggtt atatcgaaac ccacgttcag gaacacggtg cctatattct
    16261 ttccggtcag ggggtttata acctcgacaa taactggatc ccggtgaaaa aaggcgatta
    16321 catctttatg ggcgcttatt ctttacaggc tggttatggc gtagggcgtg gtgaagcgtt
    16381 cagctatatt tactcgaaag attgcaaccg cgacgtagag atttaagtca taccactcgc
    16441 ctgattattt ttagctatgt tggattttgc cacggtttat tccgtggctt tttttgtagt
    16501 caattcaggt aaaaaaagat gcgatataaa tcgcatccgc tttaaaggta atagtcagtt
    16561 tttaattccc tgacctattt taatggcgca ggcaatattt cgcgcactgt tgaagagatt
    16621 ggtttcaccg ctggcgagca cttcggctaa aggagccaga cgtggcaaaa tgctgaatac
    16681 cgcatcaatg ccgtactggt gcaccacttc cacgccatca cccaatacgc cagcaatccc
    16741 aatcaccggg acattaaact gcttcgccac cgatgccaca cccagcggcg ctttaccgcc
    16801 tgccgtttgc gagtcgatgc gcccttcccc ggtgatcacc agtgctgcac cctgcactgc
    16861 ctgcgcaaga ttgaccgcat tcaacacaat ctcaataccc ggtttaatat ccgcattgag
    16921 gaataccgcc gccgcaatgc ccataccgcc cgccgcgcca ccacccgcca tctggcagac
    16981 gttgatttca gttagctgtt gtaaaacacg ggcgtaattt tgcagcccct gttccagctc
    17041 ttcgaccatc tccggcgttg cccctttttg cgggccaaac accgccgccg cgccgcgtgc
    17101 ccctaccagc gggttatcaa cgtcacatgc tacttcaata tgacagttcg ccagacgcgg
    17161 atcgcattca ttcatctcaa tacttgccac gcgcgctaaa ttgccgccgt tagccgccag
    17221 cggctgaccg tcggcatcaa ggaaacgcac accgagcgcc tgcgccatgc ccataccgcc
    17281 gtcgaccgtc gcactgccgc caatgccgag aataatatgg cgaatgccat tatccagcgc
    17341 atggagaatt aactcccccg taccaaaact ggaggccagc aacggattac gcttttcagg
    17401 cacgaccagc atcaggccac ttgctgctgc catctcaatc accgccgttt tcccgtcgcc
    17461 ggtaatgcca taaaaagcat tcactttttc gcccatcggc ccgcagactt caagcttcac
    17521 gatgttaccg tccgtcgcgg cgaccatcgc ctccaccgtc ccttcgccgc catccgctat
    17581 cggcaaacag atatagtgcg catcgggaaa tatggtcgaa aacccggctt taattgcctg
    17641 acaacatttt tctgcactta agctctcttt aaaagagtct ggcgcaatga caatcttcat
    17701 tatcaatcct ttttcaacaa attattcgat gcgttcccac ggaataagat tatccataag
    17761 aacagccagt aaaattccga ccagtaagcc gttactgagc agaggacgaa gcgttaatgg
    17821 aaggtcttgc agatacacag gcggtaatgc cattaaaaat atgccgacaa ataacggcaa
    17881 tgcgagtcga taaatattgc gagcggtaaa cgttatttgc tggctaaaca ccagcgcgga
    17941 aaagagtaaa ggcagataag aaaccagcat gaccgcacta ctcactggta aagggatact
    18001 gcaaaacagt cgcgtgagtg ccggaaccag tgccaccagc aggcaaataa cgctgccata
    18061 aataaacgaa cgccgcgagt aatcgcctgt ctgggttaat aaaccaatgg atgaaacaaa
    18121 cggtgaaaat ggaattactg ccagcggcac ggttatcagt gtcataaatc cggtcgccac
    18181 aaagctgcga cgataacgcg tattccccgc gccctgctgc ggataaaaaa catccgtgcc
    18241 ccgaatcgca ccgtaggtat tgctgatatt taccagacct gtaatcaccg ccgtcagaat
    18301 aatccccggc gacaaagcac cgccactgcc gagcgggaac cactgccagt gcaactcacc
    18361 ggagagcgaa tgcgaagaag gaaagcaaaa gtaccacaac aaccagccag ttatcgtgcc
    18421 gaccagcagg ccgtaacggg caaaacgttg tggcaggaaa ataatcatcg ccagtaccag
    18481 gcacatcacc gccaccgaga gcgcgaacgg cggtaattga attttaaaat tcggggcggc
    18541 tatgccaaac ggcagcccga gcataccttt gaaaaagata gtggtcagtt gcgcacccag
    18601 catcaacata aacaagacca tcaccgacgg cgtaaataac cgtgccaggc gatggcctaa
    18661 tccgctaaaa ccaatcaaca tcgtcagcac gccggagagc gcaatcccca ccgccagact
    18721 ggtagcgata tcgttgatcg gtgtcccgcg cgatgcttcg ccaagggtga tagtcaggat
    18781 ggttccccac cacaggccgc ccggcccttc cataatagcg cgacgatgac cgcagaacgc
    18841 ctgagcgaag caggccagtg cggtagcaag aaaagcatat tgcgtgagcg taagcaggct
    18901 actttgcggc aactgaaaag cagaaagtag cgtaggagga accacaaccg tgttgcaaaa
    18961 aataaagaaa aaccactgga atcctgataa caggctttcg cggcccactg caaaattgaa
    19021 cataaacaca ccttaaccgg agaatgcccg ccacaaggac gggcattgca gggccagatt
    19081 actgctgatg tttaaggata aattgacctt tcggcgcaac agggaagccc tgttcgatgt
    19141 cgtaaatcac atcaccacgt aagatggttt tcgtgatacg cgcgccaata gtacggccaa
    19201 catacgggct gactttgtgg cgatattcca gatcgtcatt ggtaagaaca tagctgctat
    19261 tcggctgaat gaagacgaag tcggcatctt ttcctggggc gatacggcct ttttgctgca
    19321 gaccgaaaat atctgctgcg ttagtcgcca ttaatttgcc gaacattggc agagacattc
    19381 cgcgtttctg taccgcttca tcgaacatca cgtccatgca gctttgcaga ccagcgatac
    19441 cgccccacgc tttcatgatg ttaccggctt tcatttccgg cgggcatgga gagtggtcgg
    19501 aaaccaggca gtctatttca ccgttaaaca gtttttccca catgcctttc tggttttcca
    19561 gatcgcggat cggcggtgaa cacttcgcca gagtaccgat ttcttcgaac tgatcggtat
    19621 ccagcacaaa gtaatgcggg caggattcac aagtaacatc ctgaccttcc tgacgtgcac
    19681 gagtcacttc ctcaacacct tccgggctgc tgacgtggca aacgtgcaga cggcaaccag
    19741 cgactttcgc caggtacagt acgcggcgaa tcgcttccac ttcggtaaat accggacgcg
    19801 aagccacata gtcatgggca gttacgcgac cttcacgctt cgcttcttcg cccagtgcgt
    19861 cacagatcag cgcgttttcg cagtgcacca gcactggctg gcccagttcg cccagcttct
    19921 gcgcgccttt gaagaattgc cagtcattga cgtcacggaa gtcgttgtcg ataccgcgat
    19981 cgccacaggt cgcaacgaag catttgaaac cgacaacacc cacttcatcc aactcatgca
    20041 gacggtcaat gttgtaagac accaggccac cgagttgtgc cgcatcgata gtcagctttc
    20101 ctttagcggc atcgaacttc agttcaattg aggcgcggtc aaccgttgca ggcagctggt
    20161 tgagcggcat ttcgatcatg gtggtgatac caccttttgc tgctgcgcga gtaccggttt
    20221 cataaccttc ccagtggctg cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc
    20281 ccggcgaaac caccagacca gacgcatcca taacgtcttt tgcatcgccc agatcctggc
    20341 cgatagcagc aatttttccg tctttaacgg cgatatctac aacgcgagct tcgttttcta
    20401 aaataacggt gccgttttta atgattaaat caaaagacat aacaaactcc ttgtaaaata
    20461 cagattggtt tttaactcaa tgttcaatat cgtgattaat taacctatgg ttttttgttc
    20521 tcctgttttt tctgctgttg tacgtttctt taataaggcg taggccgcaa aggcgacgat
    20581 gacgccgaca aaccatgaaa cacgtgataa cggttccata aagggaataa acttaccgcc
    20641 aagagataaa ataacggcca ccagagttac tgaaaacgcg gtgaggttaa aaccgttatc
    20701 gtaatattta taatcgccag gtgcggtgta cagttcatca agattaattt gcccacgcat
    20761 caccacaaaa taatgcgcca tcatcacacc aattaccgga ccaagcattc cgccgataat
    20821 atcgaggaac agataaatac tgtcctgatt ttccattaat ttccacggac agatcagcag
    20881 gctgataata ctggcaatca gtacgccgtt tttataggtc agtttcgtcg gtgcaatggc
    20941 ggcaatctga taaccagccg gaataatatt accggtggcg ttagtagaga tagtcgtcat
    21001 cagaataacc agtaccgcaa agaacgaggc gaacaggctg tcccaacgct gaacaatatc
    21061 cagcacgttc caggtgtcag cgccgtaatg aatactggct ccggcaataa tacatacccc
    21121 ggcgaccgca aacagaatat aggccacaac taaacccagc gtttgcccca gcgcctgctc
    21181 acgaaacgag tgggcgtttt gcgtaaagtc ggatgcgctc accgccggtg ccgcccagac
    21241 cgcaaccacc gcgtttatca ccaccagaaa caggaagcca ctgttttctg ctttctgaat
    21301 accgcccgga atgtagtcaa agattggacc gatcccgacc agcgaaatcg cccaaatcgc
    21361 cataccgccg aaaacgatat agatgcacgg gttaagaatg gcagtgaatt tatttaaaac
    21421 ttttccgccg ccaaaaccaa taccaacgtt aaccagccag aagagtaaga aagtaattaa
    21481 tcccggtaga gaaaggccta acagcgtgaa atcgccaccg agagtcaaaa atcccggcca
    21541 gattttgcca atcagaatca agcaggccag tgaccccgcg taacattgca ggccaaacca
    21601 cataatggca gcaataccgc ctcttaataa tccgggaaac agtgcaccac gtacgccgta
    21661 agaagcacgc aggatcatgg caaacggtac gccgtattta ctgcccgcag caccgtttaa
    21721 taccattacc gcggcaatga aaaaggcact gaggataatt gccagcataa tactaaaggt
    21781 agacaagccg agaataaaaa agccgccaac catcacataa ttgggaacgt tatgaaccga
    21841 acccatccat aaggtaaaat agttaaatgt tttccaggtc cgctgacttt gcgttttcgg
    21901 cagtagatct tcgctatagc cgcgttgctg gaatagtttt ctctgatgtt ccataaatcc
    21961 ctcttaattt cacgtatcga ataacgttgt tttaatctag ctatgaggaa caaataaaat
    22021 aaaaatatta tttttaatac tgaagcaaca tctttggctg ctttcttttt ttaatattgc
    22081 tggccgattc attttctgat atcggccatt tttattgcgg gtatcaggcc agtttatggt
    22141 tagccattaa ttccagcgcc tgcactaatg cagagtgatc caactggctg ccaccgtttg
    22201 ccgcacaggt attaaacaat tcctggcagg tcgcggtgtt tggcaggttc agcgccagtg
    22261 ctttcgcact ttgcagtgcc aggttgagat ctttctggtg cagcgcaatt ttgaaacctg
    22321 gattaaaggt gcgtttaatc atgcgctcac cgtgaacttc cagaatacgt gaggaagcaa
    22381 agccgcccat cagcgcctgg cgcacacgta ccgggtccgc accggctttt gaagcaaaca
    22441 acaaggcttc agaaaccgct tcaatattga gcgccacgat aatctgattt gccactttgc
    22501 aggtttgacc gtcgccgtta ccgcccacga gagtgatatt tttaccgagc agttcaaaca
    22561 gcggttttac acgttcaaat actgcttcat caccgccaac cataatcgat aacgtccctt
    22621 cacgcgcgcc gatttcaccg ccggagactg gcgcatcgag ataatcgccg cccagttcat
    22681 tcacctgacg agcgaaacgt ttagtttcaa tcggggaaat ggagctcata tcaacaatgg
    22741 ttttgccctt cagcgaggct ttggtacaac cattttcacc aaacagaacg tcttcaacct
    22801 gtggcgtatc cggcaccata ataaaaatga tgtccgatgc atctgttacc tggcgcgcag
    22861 tttcaacact aacggcgccc agtgacagta attcatcagc aaccggacca atggttgtga
    22921 catgcagttg atgaccggca cgcgccagat taatggccat cggtgtaccc ataatgccta
    22981 agccaataaa tcccagtttc ataattaacc tcttttaaat ttcgcttttc ctgaattcag
    23041 acaacacgat cccctgccaa cttagcggcc taaaaaagca ttgtctgaat agcgttacgt
    23101 tttaacggta cgggtccatc cagcgtaaac cggcttccgt ggtggtttgg ggtttatatt
    23161 cacagccaac ccaaccgttg tagtcagaat tttcaattac cgtaaagaga tattcgtaat
    23221 taatttctcc ggttcccggt tcgccgcgat gcggattatc agcaatttgc aggtgaccaa
    23281 ttttatcggc ccactgagtc atggtattgg ttaattcacc ttccatccgc tgcatgtgat
    23341 aaatgtcata ctgaattttc aaattgcagc aaccaacatc gtcaatcagt ttcagtgcct
    23401 gccgggttcc ggtgagatga aaaccaggaa tatcaaaatg gttaataggt tcaatcagta
    23461 ataaaatatc ttctttcatc agcatattcg cagcataacg caggttttct acaagcgttg
    23521 tgtgaatctg ttcactgctg aaaccagtcg gtgttttacc gaccagacag ttaatttttt
    23581 tattacccag cgcacgagca taacgaatcg ctgctgctac gccatcccga aactcttctt
    23641 cacggccagg aatacaggca ataccccgct cgcccgccgc ccagtcacct gccggtaaat
    23701 tgtgcagcgt atgttcaagt ttattactcg ccagcacctg ttttaattct tcaatgtcgt
    23761 agtcataagg gaacataaat tcaacgccgc gaaaaccaca ctgcgcagct ttctcaaaac
    23821 gggcgagaaa atcatattca ccaaataaca tcgataaatt agcagagaaa cgtaacatta
    23881 tttatctccc ttattcatag tgcatgaaac aggtttcagt cggtgcatcc gctgcgttat
    23941 cggcgatatc ttcaaattcc atgacgttat ccagttcact gcccatcgaa atattagtca
    24001 cacgctcgag aataacttcc acgactaccg gtacccgata ttgcgccatt aagattttcg
    24061 cctgttcaaa ggctggtgca atatcttccg gtttgaaaac gcgaattgct ttacaaccta
    24121 aaccttccgc tacttttacg tggtcgacgc cataaccgtt cacttcgctg gagttaatat
    24181 tttcgaacgc gagctgtacg cagtaatcca tatcaaaggc acgttgcgac tggcgaatca
    24241 gacccagata ggcgttattg accagtacat ggatgtacgg aatgttgaac tgtgcgccaa
    24301 cggctaattc ttcaatcagg aactggaagt cgaagtcacc ggaaatcgcc accacattgc
    24361 gctctggatc agcggcacaa acccccagcg ccgccggaat cgtccagccc agcgggcctg
    24421 cctgcccaca gttgatccag tggcggtctt taaagacatg cagcatttgt gccgcagcga
    24481 tttgtgacag gccaatggtg gtgacataac aaacatcgcg gccaaaggct ttgttcatct
    24541 cttcatacac gcgctgcggt ttcaccggca cgttgtcgaa gtgggttttg cgcagcaaag
    24601 tgcgtttgcg ctgctggcag tcggcgaccc actctttacg acacggcaga cgaccggctt
    24661 tttgcatctc ctgcgccact tcaaccagca gcgtcagcgc cgctttagca tcagagacaa
    24721 tgccaagatc cggacacagc acgcggccaa tttgcgtcgg ctcaatatcg atatgaacga
    24781 ttttgcgccc ttcggtgtat ttctctaccg aaccggtatg acggttagca aaacggttac
    24841 cgataccaaa caccatgtcg gacgccagca acgttgcatt accgtaacga tgcgcggttt
    24901 gcaggcccac catcccggcc atcagttcat gatcgtccgg gatacagccc cagcccatca
    24961 gcgttgggat caccggaatg ctggtcagtt cagcaaactg ttgtaacagt acagctgcgt
    25021 cagcattaat taccccgcct ccggcaacaa tcaccggacg ttcggcctgg attaacattt
    25081 ctacagcttt ttcgatctgc atacggctgg cagcaggttt gtagaccggc agcggttcgt
    25141 acatatcagg atcaaactcg atttccgcaa cctgtacgtc gaacggtaaa tccaccagta
    25201 ccggacccgg tcgaccagaa cgcatcaggt gaaacgcctg ttgcagcaca cgaggcacca
    25261 gcgccgcttc acgaactgta accgccattt tgctgaccgg tttagcaatt gcttcaatat
    25321 caacggcctg gaaatcttct ttatgcagac gggcgcgcgg agcctggccg gtaatacaca
    25381 gaataggaat ggaatcagca gatgcggaat agagcgcggt gatcatgtcc gtgcccgcag
    25441 gaccggaagt ccccagacat acgccgatat tccctgccgt tgcgcgggta taaccttccg
    25501 ccatgtgcga agcaccttcc acatgacgcg ccagaatgtg acgaataccg ccgtgcttac
    25561 gcatcgctga gtagaacgga ttgattgcag ctcccggaac gccgaaggcg gtggtgatac
    25621 cttctttctc cagcacatac attgccgcgt caacggctct catttttgcc attttattcc
    25681 tacctctatt aattggaaaa attttccaac tttcaaattt tgtcaaaaaa acccgctccg
    25741 cagagcggga tacgacagac actatagatg tgctttcagt cccaacgccg tgctgatatc
    25801 gcgggcggtg tctctgacca gctcaccctg actgacaaaa cgatcttctg tcagtcttga
    25861 tgaaggcccg gagatggaga tagcggcaac aacactacct acatcatcgt aaattgctga
    25921 agctatgcaa ttcaggccga caacatgctc ttctttatct acggtatagc ccagttcacg
    25981 cgcttgttcc aggtccttca gcaaggtggg catatccaca agtgtcgttg gcgtaaactg
    26041 ctgcaaaccg gtttgcagaa tgatgctcat caactcttct tccgccagcg gataaagcag
    26101 cgccttgccc gctccggaag catgcagcgg cagacgactg cccagcggcg cacacatcct
    26161 gaccatcgat ttacactcta actgaccaat taataccgct tcattgccgt tacggatcgc
    26221 gacattgacc gtttcgccgg aaagtaacat caggcggcgc ataaacggcc cggcgacgga
    26281 gaggacatcg cggttatgga tgtacgctgc accgacgtta aagacaccta atcctatatg
    26341 ccaccagcct aactgattgt cctgatagac aaaatccgct gcctgtaaaa ccttcagcaa
    26401 gcgaaaggtc gtggagagcg gcaaatccag attgagagag atatcgctaa ccgacgaact
    26461 tcccccactt ttttccagat attgcagaat cgcaattccc cgctctaacg cctgtgcgcc
    26521 cttctgtgct acaggctccg cctgtcctgg cctgccgcgc cgtctaactt ccgtcatacc
    26581 tacgttaact ccgacctaca aatgagcata aacaatgaac aattcgttta tcttatgcaa
    26641 gtcggttgca ggcgtcacgc aaaacagagt tcctgttcag gaatactttc aacatcacag
    26701 ttgtcactgc cgccgcgatc gatggtcaga aaatcggtga cgcgctgcca ggcgaaaagt
    26761 gggtgatgcc agacgttacg atggtaattc actccctgtt cgccattggt aataaacgcc
    26821 cgcagggttg acaggtctgg tttgtcgtca cctaacgcca ccaccaccac aaacacttca
    26881 cctttcatcg ggataaacgc ctgtgtaccc agcggatgac gttcgagttc gtgaatggtc
    26941 agcggcagat tcgccggttg cgcgcggtta atgctgataa gcgtacggtc ttgctcaaga
    27001 atctcaacca gcgccaaatc gtggtaacgc tccaccagtc cattgttaat atggaaaaaa
    27061 tcccgtttct gcgtttcgat tacgtcgcca taagcactaa aggctttctg acttaacggt
    27121 aatacctgaa gtttcatctt tttgtccttg taacataatt ccgttcaacc accgccgacc
    27181 ggtcagcttg gtttcaataa caggcaatct aaaaccttaa gagaacaaca tcaacacaaa
    27241 agttggaata tttttccaaa actagattta gtccacaaat tgaacagggt tatcatgtaa
    27301 aaatactgat aaatcagagg ataagaaaat cacgcaactt gcggatttaa aaggggatac
    27361 ctgaagatgt tcgatccaga aaccttgcgg actttcattg cggttgctga aacaggtagt
    27421 ttttcaaaag cggcagaacg attatgtaaa accacggcga cgatcagtta tcgcattaaa
    27481 cttctggaag agaataccgg agtggcgctg tttttccgca cgactcgcag cgtgacgttg
    27541 acggcggctg gcgagcatct actttgtcag gccagagact ggctgagctg gctggaaagt
    27601 atgcccagcg agttacaaca ggtgaatgat ggcgtggaac gccaggtgaa tatcgtcatc
    27661 aacaacctgc tctataaccc ccaggccgtt gccaggctgc tggcgtggct aaatgaacgt
    27721 tacccgttta cccaatttca catctcccga caaatctata tgggcgtctg ggactcgcta
    27781 ttgtacgaag gtttttcact ggctatcggc gtcacgggaa ccgaggcgct ggcaaatacc
    27841 tttagtcttg atcccttagg atcggtgcaa tggcgctttg tcatggcggc ggatcatccg
    27901 ctggcgaacg ttgaagagcc gctaacagaa gcacagttgc ggcgctttcc ggcggtcaat
    27961 attgaagaca gcgcccgcac cttaaccaaa cgcgtcgcct ggcgattgcc ggggcaaaaa
    28021 gagattattg ttcccgatat ggaaacgaaa atcgccgccc atctggcggg cgttggcatt
    28081 ggttttttgc caaaatcact ttgccagtca atgctcgata atcaacaact ggtcagccgg
    28141 gtaatcccaa cgatgcgccc tccttcgcca ttgagtctgg catggcgcaa atttggcagc
    28201 ggcaaagcgg tagaagatat tgtgaccttg tttacccagc gcaggccgga aatcagcgga
    28261 tttttagaaa ttttcggcaa cccacgcagt taaaaatcaa gtttccttgc gcgttggcgc
    28321 gtaaaatagc gcgcaagctg atttgaggtt cactttttac tatgcaagag agacacacgg
    28381 aacaggacta tcgtgccctg ctgattgctg atacgcccat tattgatgtt cgcgccccca
    28441 tcgagtttga gcaaggcgca atgcccgccg ctatcaacct gccgttaatg aataacgatg
    28501 aacgcgccgc cgttggcatc tgctataaac agcaagggtc agacgcggcg ctggcactgg
    28561 ggcataaact ggtggcgggt gaaattcgtc agcagcgcat ggacgcctgg cgggcagcgt
    28621 gcctgcaaaa tccgcacggt attctctgct gcgcccgtgg cggtcagcgc tcacatattg
    28681 tgcaaagctg gttgcatgac gcggggattg attatccgct ggtggaaggc ggttataagg
    28741 cactgcgcca gaccgcgatt caggcgacta ttgaactggc acaaaaaccg atagtgctga
    28801 ttggcggttg taccggcagc ggtaaaacgc tgttagtgca gcaacagccg aacggtgttg
    28861 atctggaagg gttggcgcgt catcgcggtt cggcgtttgg tcgcacctta caaccacaac
    28921 ttagccaggc gagttttgaa aacatgctgg ctgccgaaat gctaaaaacc gacgcccatc
    28981 aggatttgcg cctgtgggtg ctggaagatg aaagccggat gatcggttcg aatcacctgc
    29041 cggaatgtct gcgcgaacga atgactcagg cgacgattgc ggtggtagaa gatccgtttg
    29101 agatccgtct tgaacgcctg aacgaagaat atttcttgcg tatgcatcat gattttaccc
    29161 acgcttatgg cgacgaacag ggctggcagg agtattgcga atacctgcat cacgggcttt
    29221 cggcgattaa acgtcggctg ggactacaac gctataacga actggctgca aggctggatg
    29281 cggcgctgac aacgcaactc accaccggca gcaccgacgg tcatctggcc tggctggtgc
    29341 cgttacttga agaatattac gacccgatgt atcgctatca gcttgagaaa aaagcggaaa
    29401 aagttgtctt tcgcggtgag tgggcggaag tggcggaatg ggttaagact tatgcctgtg
    29461 gttattaacc cacaaaaatg cttttccgtt cctctttaat aaattgtgca actggttaac
    29521 taccagtt
//
LOCUS       DAOFSV010000048        28966 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.048, whole genome shotgun sequence.
ACCESSION   DAOFSV010000048 DAOFSV010000000
VERSION     DAOFSV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.048"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..453)
                     /locus_tag="QBH27_002225"
     CDS             complement(<1..453)
                     /locus_tag="QBH27_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014531.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGL"
     gene            complement(731..2095)
                     /gene="tdcG"
                     /locus_tag="QBH27_002226"
     CDS             complement(731..2095)
                     /gene="tdcG"
                     /locus_tag="QBH27_002226"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDQ7014532.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(2167..2556)
                     /locus_tag="QBH27_002227"
     CDS             complement(2167..2556)
                     /locus_tag="QBH27_002227"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDQ7014533.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2570..4864)
                     /gene="tdcE"
                     /locus_tag="QBH27_002228"
     CDS             complement(2570..4864)
                     /gene="tdcE"
                     /locus_tag="QBH27_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDQ7014534.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDTYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4898..6106)
                     /gene="tdcD"
                     /locus_tag="QBH27_002229"
     CDS             complement(4898..6106)
                     /gene="tdcD"
                     /locus_tag="QBH27_002229"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDQ7014535.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(6132..7463)
                     /gene="tdcC"
                     /locus_tag="QBH27_002230"
     CDS             complement(6132..7463)
                     /gene="tdcC"
                     /locus_tag="QBH27_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDQ7014536.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7485..8474)
                     /gene="tdcB"
                     /locus_tag="QBH27_002231"
     CDS             complement(7485..8474)
                     /gene="tdcB"
                     /locus_tag="QBH27_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDQ7014537.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8573..9511)
                     /gene="tdcA"
                     /locus_tag="QBH27_002232"
     CDS             complement(8573..9511)
                     /gene="tdcA"
                     /locus_tag="QBH27_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDQ7014538.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTATLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            9826..10044
                     /gene="tdcR"
                     /locus_tag="QBH27_002233"
                     /pseudo
     CDS             9826..10044
                     /gene="tdcR"
                     /locus_tag="QBH27_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
     gene            complement(10610..10986)
                     /gene="rnpB"
                     /locus_tag="QBH27_002234"
     ncRNA           complement(10610..10986)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="QBH27_002234"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(11019..12164)
                     /gene="garK"
                     /locus_tag="QBH27_002235"
     CDS             complement(11019..12164)
                     /gene="garK"
                     /locus_tag="QBH27_002235"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDQ7014539.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(12261..13151)
                     /gene="garR"
                     /locus_tag="QBH27_002236"
     CDS             complement(12261..13151)
                     /gene="garR"
                     /locus_tag="QBH27_002236"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDQ7014540.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(13181..13951)
                     /gene="garL"
                     /locus_tag="QBH27_002237"
     CDS             complement(13181..13951)
                     /gene="garL"
                     /locus_tag="QBH27_002237"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDQ7014541.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(13967..15301)
                     /gene="garP"
                     /locus_tag="QBH27_002238"
     CDS             complement(13967..15301)
                     /gene="garP"
                     /locus_tag="QBH27_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDQ7014542.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            15676..17247
                     /gene="garD"
                     /locus_tag="QBH27_002239"
     CDS             15676..17247
                     /gene="garD"
                     /locus_tag="QBH27_002239"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDQ7014543.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            17396..17731
                     /gene="prlF"
                     /locus_tag="QBH27_002240"
     CDS             17396..17731
                     /gene="prlF"
                     /locus_tag="QBH27_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDQ7014544.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            17731..18195
                     /gene="yhaV"
                     /locus_tag="QBH27_002241"
     CDS             17731..18195
                     /gene="yhaV"
                     /locus_tag="QBH27_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDQ7014545.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(18250..19059)
                     /gene="agaR"
                     /locus_tag="QBH27_002242"
     CDS             complement(18250..19059)
                     /gene="agaR"
                     /locus_tag="QBH27_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDQ7014546.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            19308..20588
                     /gene="kbaZ"
                     /locus_tag="QBH27_002243"
     CDS             19308..20588
                     /gene="kbaZ"
                     /locus_tag="QBH27_002243"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDQ7014547.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPAGFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            20611..21084
                     /gene="agaV"
                     /locus_tag="QBH27_002244"
     CDS             20611..21084
                     /gene="agaV"
                     /locus_tag="QBH27_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDQ7014548.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            21095..21874
                     /locus_tag="QBH27_002245"
     CDS             21095..21874
                     /locus_tag="QBH27_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDQ7014549.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            21864..22742
                     /locus_tag="QBH27_002246"
     CDS             21864..22742
                     /locus_tag="QBH27_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDQ7014550.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            22760..23194
                     /locus_tag="QBH27_002247"
     CDS             22760..23194
                     /locus_tag="QBH27_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ7014551.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            23191..24324
                     /gene="nagA"
                     /locus_tag="QBH27_002248"
     CDS             23191..24324
                     /gene="nagA"
                     /locus_tag="QBH27_002248"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDQ7014552.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQQT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMHGGIVRTAFGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            24674..25828
                     /locus_tag="QBH27_002249"
     CDS             24674..25828
                     /locus_tag="QBH27_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDQ7014553.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAITAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVTIHPWQA"
     gene            25841..26701
                     /gene="kbaY"
                     /locus_tag="QBH27_002250"
     CDS             25841..26701
                     /gene="kbaY"
                     /locus_tag="QBH27_002250"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDQ7014554.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSRDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            26868..27344
                     /gene="agaB"
                     /locus_tag="QBH27_002251"
     CDS             26868..27344
                     /gene="agaB"
                     /locus_tag="QBH27_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDQ7014555.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRYIKQRGVNVFIQDVPGDQKEQIPD"
     gene            27383..28186
                     /gene="agaC"
                     /locus_tag="QBH27_002252"
     CDS             27383..28186
                     /gene="agaC"
                     /locus_tag="QBH27_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDQ7014556.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            28176..>28966
                     /gene="agaD"
                     /locus_tag="QBH27_002253"
     CDS             28176..>28966
                     /gene="agaD"
                     /locus_tag="QBH27_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDQ7014557.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKNDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
BASE COUNT         7294 a         7467 c         7082 g         7123 t
ORIGIN      
        1 caacccttcc gtcacgccga aggtatgcag gtaggaagca ctatcgttgg tgatggcggt
       61 ggaatagacg aacatccaaa tcaccagcat tacgaaatag agtgcaccta acaggatgcc
      121 ccagttttta cctaaataac cgctgatgac gctcgggtaa tctttacatt ctggtgattc
      181 tgccagcgta ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga
      241 tgagagcaaa aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc
      301 cgcgccaatc gccatcccga tactcataat cacccagcca gtgtcagtac tgtcgaattt
      361 gatcgcttct cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc
      421 tgcaatgacg cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc
      481 ttattatttt tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt
      541 tattagaata aagaaatgat acgcgtgaga tcagagaaaa tcttcacaat caagactttt
      601 aaatagcaat tgtgggtaat aattttctat taaattgagt taatagaaat aattattctc
      661 tatttgcggc agatcacaat aaaaaagggt gcacattttg tgcacccaag gatgatagct
      721 gacagcaatg tcagccgcag accactttaa tagccagtcc tccgcgtgat gtttcgcggt
      781 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
      841 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
      901 taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
      961 gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccac
     1021 ccagtagttc agttaaccct gccgctgcca ttgaacaggc cacgccaatc tccccctgac
     1081 agccgacttc cgcgccagag atggaggcgt tcattttata cagcgcgcca attgcccccg
     1141 ctgccagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat
     1201 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
     1261 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat
     1321 tcatcggatc gttagagatg ttatcgctgg aaaccagttg acgacgcagc gctacggcac
     1381 ggcgcggcac attgagcgga ccaggcagca cgccttcagt attcattcca cgttcaatac
     1441 cgtcatgcat cacttgccag atacgggcaa accctgcgtc aatttccgct ttactgcgca
     1501 gcgccagctc gttgtgcatc atcaggccag atatagacag gccgttgtaa tcacacattt
     1561 tcagcagttc acctgctgag tggaaatcgt aaggtacggg tgtttcgaca tcgtgcgaca
     1621 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttgctta
     1681 atagctcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
     1741 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcgccagaag
     1801 ccaccggcaa ccgcccgctg cgcgtcacta actctataaa tgcagggatc tcatcaatga
     1861 caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc gtggcatggc
     1921 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
     1981 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacag
     2041 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
     2101 caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac
     2161 gaggctttac gcactacgta ctgcgatggc ttcaatttcc agcttcacat ctttcggcaa
     2221 acgcgcgacc tgcacacagc tccgggtcgg gtaggtcgcc tgatgttcat cgaagaactg
     2281 cttatagact tcattgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
     2341 gatgatatcg cccacactca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
     2401 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc aaaccggaat
     2461 ttgcccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
     2521 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
     2581 gggtaaaggt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca
     2641 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
     2701 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
     2761 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt
     2821 ctttgcccag cgccgcaggg acgatggaga aggtgtacga gatcccatct ttggcgtagg
     2881 tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcga tcacgaccgt
     2941 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg gcgaccgtcc ggcgtgttcc
     3001 cggttttctg accgtacacc acgttagagg tgatagtcag aatcgattgg gtcggcacag
     3061 cattgcgata ggttggcaaa gctttaattt tcttcataaa gcgttcaacc agatcgcagg
     3121 caatgctgtc tacgcgctcg tcgttgttgc catactgcgg atattcgccg tcaatctcga
     3181 aatccaccgc caggccgttt tcatcacgga ttggtttcac acgagcatat ttgatggcag
     3241 acagggagtc cgttgccacc gacaggcccg cgatgccgca cgccatggtg cgatagacat
     3301 cacgatcatg tagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
     3361 tgatattcag cgcgctgatg tactgtaccg ccagccagtc catgaagtgg tcgaggctat
     3421 ccatcacttt gtcgtagtcc agcacgtcat ccatcaacgg agccgttttc gggccaacct
     3481 gaatcttcag cttctcgtcc attccgccgt tgattgcgta cagtagcgtt ttcgccaggt
     3541 tagcgcgcgc accgaagaac tgcatctgct taccaatcac cattgggctg acgcaacagg
     3601 caatcgcgta atcgtcgcta ttgaagtcag tacgcatcag atcgtcgttt tcatactgca
     3661 aagaggatgt gacgatagac acctgcgcgg catatttttt gaaggcaatc ggcaaggctt
     3721 ccgaccacag aatagtcagg ttaggttccg gtgcaggccc catagtgtgc agggtgtgta
     3781 aatagcggaa ggagtttttg gtcaccaggg ttcgaccgtc caaccccatc ccgccgatca
     3841 cttccgtcgc ccagatcggg tcgccggaga acagcgaatc aaattccggc gtacgcaaga
     3901 agcgcaccat acggatcttc atgatgaagt ggtcgatcag ttcctgtgcc tgctgctcat
     3961 tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcgcc
     4021 ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc caggtaagcg aagtagagcc
     4081 actgcacggc ttcttgtgca ttctgcgctg gacgagagat atcaaagcca tatttcgccg
     4141 ccatttcctg aatctgtaac agcgcacggc gatgttctgc cagttcctca cgcagacgaa
     4201 tggtggcttc gagatcttcg cctttttcca gacgggactg gagatcggca aactgcagtt
     4261 cgcgttcacg taccagataa ctgatgccgt acagtgccac acggcgatag tcaccgatga
     4321 tacgcccacg accataacca tctggtaaac cggtcagtac accagattta cggcagcgca
     4381 gcatatccgg tgagtaagta tcaaatacgc cctgattatg ggttttacgc agatcggtaa
     4441 agatgtattc aaattcgcta tccatttcac gaccatacgc gtggaatgaa cttctaatca
     4501 tattaatgcc gccgaacgga tgcagcgcac gctttaatgg cgcatccgtt tgcagaccca
     4561 ctattttttc cagcggctgg ttaatatatc ccgcgtcatg agcggtaatt gtggtggcaa
     4621 tattggtatc gaaatccaat ggcgcgtgag tcgcattttc gatacggatg ccttccatga
     4681 ctttttccca caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt
     4741 cataaggtgt atagttatgt tgaataaaat cgcggacatt aatttcgttt ttccagtccg
     4801 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
     4861 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt
     4921 tcactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaatta
     4981 cggcgcaaat gacacgcgca ttttcactgg aaacaattcg ctctccaaaa gagttagagc
     5041 gattattcat ttctgtatca atttctacgc ctaatacagc taaatgttcc atgaccagac
     5101 gacgaattaa acttgaattc tctcctattc cgccggtgaa tataattcca tccaggcgat
     5161 gtaatgaagc agcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg
     5221 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
     5281 aaaggccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtccccca
     5341 ggctctggtt ggtttggctg gcgacccagg acatcgcccc gaagtcgaca tcaccactgc
     5401 gggtacccat catcaagcct tccagcggcg tcattcccat tgaagtgtca acactctgac
     5461 cgttgcgaac cgcgcagatt gatgcgccat tgccaagatg cgccacaacc aggccggagt
     5521 catcttccgc cagattcagc agcgaatgtg cgcgctggga aacatagcgg tgcgacgtgc
     5581 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
     5641 aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta
     5701 cgcccgggaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
     5761 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
     5821 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaagccaca ctgtcattta
     5881 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
     5941 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
     6001 cggcaatacc tgacattaat acttcacagt cgctggcatt gagtacggaa aacttaatcg
     6061 aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc
     6121 tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg
     6181 gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg
     6241 gcatacatcg gcaacaggca cagcagtgat gcgataatcg gtgcgcccat ggcttcaatc
     6301 aagtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat aatgaaaatc
     6361 atgctgatag tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac
     6421 ttcagaatca aaccattcag cccttccagc gtccccagat agtgaccgaa gaaagatttg
     6481 aaaatagcca cgagtgcgat gatggaagcg gcatattcca gtgtaatcgc aaacgttgtt
     6541 ttggtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc
     6601 gctttcgctt cagccatatt cgccggagac agcgtaaaca ggcagctaaa ggcaaagaac
     6661 atcaccactg caaccatcag catactggcg cgagagatga tttgtgaaca tttacgctcg
     6721 gtgaagtcgc gaccaaaatc tttctcgtac tcttcacgtt tagaaaccac gaaggaagag
     6781 acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacggtgatc
     6841 aggataccgt catgaccagt taacgacagc gaaccgaggt caacctggtc gataactgca
     6901 gagttccagt aagggatcag cgaaagagaa atcagcacca ggctggcgat aaacggccat
     6961 accagatagc tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc
     7021 agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt
     7081 tcccagaacg tcataaaggt gttggtaatg gtaacgccat aaatccacaa cagtgggcaa
     7141 atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc
     7201 accgtttccg taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagtgcc
     7261 cggtggcaat aaaacgcgat ggggtatgct aataccaaca tcagaagaat cgggatcagt
     7321 ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac
     7381 aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat
     7441 acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa
     7501 ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt
     7561 tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg
     7621 cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt
     7681 tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa
     7741 ttacccgggc gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtagtt
     7801 atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttcagactg tacgccaata
     7861 acacggatgg tcgggttgat agatttaatt gccaccgcaa taccagcaat taaaccgccg
     7921 ccgccaattg gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca
     7981 atcgttccct ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacggcct
     8041 tccatttcga caatttcgct cactttagca atagtgtcgt tgaagttatc accatgcaaa
     8101 acgacttctg cggagtagtc gcacgttgcc gcgactttgg atttcggcgc accttttggc
     8161 atcaccactt taccgtcaat acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg
     8221 ttgcccgcag agcaggccac tactccttta cgtttttccg catcagtcag tgaacttaat
     8281 ttattaaatg cgccacgaat tttaaatgaa ccagtacgct gcatgttttc gaatttgagg
     8341 aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatacc tgttttataa
     8401 attcgcccag ccagtcgttg ttttgcttca atgatgtcat caatagcaac cgggagatcg
     8461 tatgtaatat gcattataaa acctcttagc ctgaattaaa tatgtaggta accgtaaccg
     8521 acacctgcaa aacagacagg tggattattt atgtttaaat taaaacaaat aactaatcaa
     8581 cttcaattaa ttgccttcgt ctacacccat tataagatga atactctttg gctaattcca
     8641 ccaaaaccga tgctgctttt ttaatacgat aatttttcga ccataccgcg gcatattgtg
     8701 ccacaggtaa tgtttcttca accggaatag taataaattg attagaacca aaaggtgatg
     8761 tcatatcaca aggaattaca gttaagaaat cagcattgag aacaagatta taaattgtca
     8821 cgactgagtc ggttttaacg atgttttcaa tactgatgcc atttctttgt aacgtagtaa
     8881 gtagttcgct gtagtacccc atattggttt gtggcaacac ccactgttcg ttcttcaacg
     8941 actccagcgt ggcggtgccg gtgcatgttc gggacttact ggctaccagc acaaactcgg
     9001 actcgaacag cggctcaaca tgtaaatcct gaagcttcat ttctgcgctt aacgtaccaa
     9061 tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat
     9121 acatagaaac ctgcgctttc gggaacacct ctttgaactt gttgatcatc cctgacataa
     9181 aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc caccaccgcc tcagaagaca
     9241 taccgcttat ctcattaacc atatttttca tttcacgggt aatggattcg gaacgggaga
     9301 gtaacaattg accggcaggt gttaatgtta caccggtatt tttccgcacc actaactcca
     9361 ccccaaaata atcttcaata tcgttaatga ttttactgac ggccggttga gttaacccta
     9421 actcttttgc agccgagccg atagaaccac ttctaataac ttcctgaaag actaccaggt
     9481 gctgcgtttt cggaagaaga atagtgctca taacgaccta cgttaattac ctcattgacg
     9541 gtatgaagtg tatcaaaatg aaatgaacag gatatgtgcg accactcaca aattaacttt
     9601 caatactttc catagcatcg ttaccccaat taaatacctt taagtatcaa caagttaaag
     9661 tatgaaaatc agcataaacc ctgatttttg tcaaaaaaaa tgacggggat aaccatattt
     9721 tatggcgata acatcattct ttatgtggtt aatacaaaaa aggggctgag actatatttc
     9781 aagcaattac cggataatta ccaggcaaaa tttgagttaa atttaatgag taagttctca
     9841 aattttatta taaacaaacc attttcagcg ataaataatg cggcacgtca cattttttaa
     9901 cgctatttgt tggagaacaa acatttattt tatcaatatt ttaaaatttc gaatacaggt
     9961 attgatcatc tcgaacaatt gattaacgtc aacgttttct cttctgacag gatgtcattt
    10021 tgtgaatgca atcgttttcc ataaattctt ctcccatcat aggcaacgaa gagcattttg
    10081 tgttgaggat cacaaaacga ataattgctg gtcgccgcga taaggtcaga cgaagacaac
    10141 aagggggatt ctcactgtgc tttacatcag cgtaggccat agaatgttgc ttcgttgact
    10201 gtaatgccgg gtaaatgagc gttttttgat agtgcgaaag aaccgtgcaa ggagaataag
    10261 catctattat tgtttgtcat gatgacaagg caggcatcaa tgaaaggctt ctcaattgca
    10321 catattcttc acccttcaat cccgtcaatg cacgcagtgg ttaacaatca caatagaaat
    10381 attgattatt ggacggtaaa aatttaggtt ataacgccca aattattcaa cgatcggaca
    10441 atacctgcga acttacaatt aatgagagaa atatacgtat aattacgacc ttttaaatgc
    10501 aatatagact taataacttc ttacaaagtc tgttattaat accgtagata ttcatcttac
    10561 cctgggcaca caaagcaaaa acccgccgaa gcgggttttt acgtaaatca ggtgaaactg
    10621 accgataagc cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg
    10681 ctcaagcagc ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg
    10741 ctccgggtgg agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc
    10801 accctttcac ccttacctga tcccgcttac gcgggccatc ggcggtttgc tctctgttgc
    10861 actggtcgtg ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact
    10921 ttcctcccct ccgcccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc
    10981 agcttcggcg cgcagtatag agggtttgcg cgcccttgtc accccgcgtt gcgcattcca
    11041 atcgccagtg tcgcggcgat attacgtgaa gcacggcaga tattgtcata agctccgcgg
    11101 agtgcttcgt ctaacgtacc aatgctggtc agtacgctga agaccgcatc aatgccatgc
    11161 tgatgtacaa cgccaacatc attggtcagg ctacccgcaa tgccaatcac cggtttatgg
    11221 tacttcttcg ccacgtttgc gacaccaatc ggtaccttgc cgtgaatact ctggctatca
    11281 atacgccctt caccagtgat caccagcgta caatcgtgaa tatgttcctc cagattcagc
    11341 gccgtagtga cgatttcaat accacttttc agttctgcac caagaaacgc catcagcgcc
    11401 gcgcccatac ctcccgcagc tcctgcgccg gggacatctt tcacatcaac atgcagcgct
    11461 tttttaataa catcggcata gtgagagagg ttattgtcca gctcgacaat catcgcctca
    11521 ctggctccct tttgtgggcc aaagatgcgc gaagcaccac tgtcgccgac taacgggttg
    11581 gtgacatcac aagcgacgcg gatgacacaa tcttttaagc gcggatcgag accggaaata
    11641 tcaatatcat tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggca
    11701 tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc cgccgtcgtt ggtggcgctg
    11761 ccgccaatgc cgataataat gtttgtcgca ccgctctcca gcgcctgcag gattaactca
    11821 ccagtaccgc gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac cagttccagc
    11881 ccactggccg ccgccatttc gataaacgcg gttttgccat cgccggagat cccccaactg
    11941 gcattcactt tctcgcccag cggccctgta acccaggcgt gacgttcagc cccctgggtg
    12001 gctgcaatca tcgcttccac cgttccttca ccaccgtcgg caaccggaac agaaacatac
    12061 tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca
    12121 gataaacttt ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc
    12181 acatgacgcc ggagggcgtt tctcttattc ggcctggatt ccaggcccgg attgcaatac
    12241 gccatccggg cacgacgtca ttagcgagta acttcgactt tcgccagttt ttcgtagtag
    12301 cacgccaggg cgctatgatc cgccgttcct aaaccatctg cacgcagtgc ctgcatcatc
    12361 tccataaccg cagcagtgag cggcagttgc gcgccgacgc cgtgagaagt atccagcgca
    12421 ttcgccagat ccttaatatg cagatcaata cggaagcccg gtttgaagtt gcggtccatc
    12481 accatcggcg ctttggcatc cagcacggta ctgcccgcca gcccaccgcg aattgcctga
    12541 tacaccagat ccgggttaac gcccgctttg gttgccagcg ttaacgcttc tgacatcgcg
    12601 gcaatattca gcgccacaat gacctgattt gccagtttgg tgacgttacc tgcaccgatt
    12661 tccccggtat gcaccacgga acccgccatc gctttcatca aatcatagta tttgtcgaaa
    12721 atagccttgt cgccgcccac catcactgac agcgtaccgt cgatggcttt cggttcaccg
    12781 ccgctcaccg gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca
    12841 cggcttgcca gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct
    12901 tcaataatgc cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg
    12961 gttatgatga cgtcgcactg ttcagcgatc gctttagccg tagcggctgt ttctgcaccg
    13021 gcagcaatca cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct
    13081 gctttcagaa ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact
    13141 ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag
    13201 ccagtttctg agtggcagag cggaagacgc cgagatcgct gccgacggcc acaaacgtcg
    13261 cgccccattc cagataacga cgcgcatcag cctcgaccgg tgcgaggatg ccgctgggtt
    13321 tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca tccgggtgcg
    13381 aggcattgcc gagatggcct aatgccgcag ccagatcgct ggggccgacg aagatgccgt
    13441 ctacaccttc ggtagcggca atagcatcga cgttatctac gccctgctga ctttctatct
    13501 ggaccagaat agtgatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca
    13561 tattggcgcg gtgagaaacg gagacgccgc gaatgccttc cggtgggtaa cgagttgatg
    13621 ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac
    13681 cgatatccag aagacgctta ataattaccg gctcgttggt cggcactcgc actactggcg
    13741 cgctggcgct gcctttcagg gccattaact gcggaataaa cgtggagata tcgtttggcg
    13801 catgttcacc atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa
    13861 tcgggttaga gagagctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt
    13921 tgaatttatt cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt
    13981 ccatacgttt aatgtcgccg actacgaaga ggtagcagac catcgccatc agcgctgaac
    14041 atcccacgaa aaccagtgct gcattgaagg agtgcagttc acttaccaga tagccaatca
    14101 ccagtggagt gacaatggag gcaacattgc caaagacgtt aaagacgccg ccgcagaggc
    14161 caacaatctc tttcggcgcg gtgtcagaaa tcactggcca gcccagcgca ccaaatcctt
    14221 tgccaaagaa agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac
    14281 ataagatgat ggtggaagcc agcaacattc ccagcacaat tggtagctta cgtgccaggg
    14341 tcagggacaa accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg
    14401 caaagccaca cagtgccgga atcgaggcga ccagacccac tttcagaatc gacatgcctt
    14461 tttcctgcac cagataaatt gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa
    14521 aatattgtcc gaaaaatacg cccagcatca tgcggttaga gagcaattgc ttgatgtaat
    14581 gcagtttggg tccgcttgct gccgcactac ccggcttttt gtggtccata tcgaccaccg
    14641 cgccattttc cgagataaac ttcagctctt ctgcagacat acgtgggtga tctgtcgggt
    14701 tatgaatcaa cttgatccac agcgccgtca acacaaaacc aatcaccccc ataacggtaa
    14761 agacatgctc ccagccccag gcgaaagtca gccagccaag cagcggcgaa aagagcgcca
    14821 acgagaaata ttgcgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga
    14881 accaggcggc gacaatacgg gcgttcgccg ggaatgatgg cgcttccgag aaccccagca
    14941 taaatcgcat aaagaacatg gagatcccag cccaggccag cggaaacata tcgacaaagc
    15001 cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta agtgtaaact ttcttcgagc
    15061 caaacttatc aagcagccag ccgccgggaa tttgcatcag caagtaggcc cagccaaaag
    15121 cagagaagat gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac
    15181 cagcaataga cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca
    15241 gtaatattaa atagcgggta tgcacgcctt tcttttttac gtcaacggtg tccagaatca
    15301 ttttatttac ctcgggtact tatgctgatt tttattatta tggggaaggt gttatttatg
    15361 agttttattt atgccgttac gacaatgaac tcgggaatta gtataagcag cgcgagaata
    15421 ataatcattg tgcaaatgct aatttaatta gtactattta aatattattt tgagcatatg
    15481 cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttgccggga ggtactgcaa
    15541 tccctgaaat gattgacatt gatcacattt ctgcatttaa actcctgaca ttcttatttc
    15601 acccaatgaa gttatttatt tttaaatgag accaggtcct cattttaata gtccctggct
    15661 ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat
    15721 ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca
    15781 ggaacgcgtt ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc
    15841 gcattgctgg acattccggc taatggtgaa attattcgtt atggcgaggt gattggttac
    15901 gccgtgcgcg caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa
    15961 gcgccgccgt tacatacgct gccactggca accaaagtcc cggaaccctt accgccgctg
    16021 gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg
    16081 ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactacgt agtaaaaatc
    16141 attgaacgcg atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat
    16201 ttgtacggtt gtggcgtggc gattaacgca cccgcggcag ttgtacctat tcgtaccatt
    16261 cacaatattt cgctgaaccc taactttggc ggcgaagtaa tggtgattgg tctgggttgt
    16321 gaaaagttgc agcctgaacg cctactgact ggaacggatg atgtgcaagc tattccagta
    16381 gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc
    16441 gaggatattt tgcaggtagc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa
    16501 acctgtccgg cttcagaact ggttgtcggc atgcagtgcg gtggcagcga tgcgttttct
    16561 ggcgtaacgg caaacccggc ggttgggtat gcgtctgatc tattggtgcg ctgcggcgca
    16621 acggtgatgt tttcagaagt cacagaagtg cgtgacgcga tccatctgct gacaccacgc
    16681 gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat
    16741 ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt
    16801 ctggcaaacg tggtagagaa ggcactcggc tccattgcta aatcgggtaa aagtgcaatt
    16861 gttgaagtgc tgtcgcccgg ccaacgcccg actaaacgcg gattaattta cgccgcgacg
    16921 ccagccagcg attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg
    16981 tttacgaccg gtcgtggtac gccgtacggc cttatggcgg tacccgtcat taaaatggcg
    17041 acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc gggcactatc
    17101 gctaccggcg aagaaaccat tgaagaagtg ggctggaagt tgttccactt tattctcgac
    17161 gtcgccagcg ggaagaagaa aactttctcg gatcaatggg gactgcataa ccagctggcg
    17221 gtgtttaacc cggcaccggt gacctgattt cttttggaac gtccctcgca aaacatggcc
    17281 ttagtgccat gtttttattg tttaaagtcc acacatccat taataatgca tttgcattac
    17341 cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacagt aaataatgcc
    17401 cgctaatgct cgctctaacg ctgtactgac cactgaatca aaggtcacta tacgcggaca
    17461 aacaactatc cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc tggacagcat
    17521 tcattacgaa attctgcctg gtgggcaagt atttatgtgc cgactgggag atgaacagga
    17581 ggatcatact atgaatgcat ttttgcgttt tctggatgca gatatccaga acaatccgca
    17641 aaaaactcgt ccattcgaca ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa
    17701 cattgacgat gagattggcg acgacgaata atggattttc cacaaagggt taatggttgg
    17761 gcgctatatg ctcatccctg ttttcaggaa acctacgatg ctttagttgc cgaagtcgag
    17821 gcattaaagg gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa gttattggcg
    17881 gtggtccata aagtgattga ggagcatatc acggtcaatc catcatcacc ggcattccgt
    17941 catggcaagt cgttaggctc tgggaaaaat aaagactggt cacgggtaaa atttggtgct
    18001 ggtcgctatc gtctcttctt tcgttacagt gaaaaagaga aagtcatcat tctgggatgg
    18061 atgaacgatg aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta taccgtattc
    18121 agtaaaatgt taaaaagagg acatcctcct gccgactggg aaagcctcac ccaagagaca
    18181 gaagaaaacc attgatggtg ttcacaatgc ccgccgaacg cattggtgtt gaccagcggg
    18241 catcctttct cattccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag
    18301 actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
    18361 cttatgtaaa ctggaacggt tgaacttact ggaatcggtg accacgatga tccgttccgc
    18421 aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgcgtgc tgacgccacg
    18481 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
    18541 ttgctcagcc tgatcaccgt aaaaagattg cgactgacgg cgcaaatgcc ccccggtcat
    18601 cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat
    18661 cgcaattacg tcggtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
    18721 gtcgaggatc acccgatgac caggctgaat caactcaacg gcagctttcg caacgctgcg
    18781 tttcatcgcg gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgttgt
    18841 gctatcgcag atcaacgcgc caccataggc acgcacggcg atcccctgct tttccagaaa
    18901 cgccagatcg ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac
    18961 ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc
    19021 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
    19081 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt
    19141 acccattggc gcaagctact ctcgtttcag tggctttcat tatgtttctt ttgtgaatca
    19201 gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag tatcaactga
    19261 aacaaaacga aagattaatg tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
    19321 cagaaatggt gagacagcac aaagcgggca aaacaaatgg gatttatgcc gtttgttccg
    19381 cacatccgct ggtgctggaa gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
    19441 tgattgaagc tacctccaat caggtggacc agtttggcgg ttataccgga atgacgcccg
    19501 ccggttttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc
    19561 tgattctggg tggcgaccat ctggggccaa accgctggca aaatctgcct gccgctcagg
    19621 caatggccaa tgccgatgat ttgattaaaa gctacgtggc ggcaggattc aaaaaaattc
    19681 accttgattg cagcatgtcc tgtcaggacg atccgattcc cttaaccgat ggcatcgtgg
    19741 ccgaacgcgc cgctcgtttg gcgaaagtgg cggaagaaac ctgccgtgaa cattttggcg
    19801 aagccgatct ggagtatgtc attggtaccg aagtgccggt gcctggcggc gcgcatgaaa
    19861 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
    19921 gtcacgcttt tgaaaagcag ggtctgaatg ccatctggcc acgcatcatt gccctggtgg
    19981 ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga
    20041 ccgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg
    20101 attatcaaac gccgcaatcg ctgcgccagc tggtgattga ccactttgcc attctgaaag
    20161 ttggcccggc gctgaccttt gctctgcgtg aagctctgtt ctctctggca gcgattgaag
    20221 aagaactagt gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc
    20281 tcgaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg
    20341 cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg
    20401 acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgctg ccgctgatca
    20461 gtcagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac
    20521 gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag
    20581 gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag tcggattgat
    20641 gaacgcttga ttcacggtca ggtcggcgtt cagtgggtag gatttgcggg ggcaaatctg
    20701 gtgctggtag ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg
    20761 gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagtcat cgacaacatc
    20821 catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacaccggc cgatttcctg
    20881 acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc
    20941 aatggcaagc aacaaatcgc caaaacggtt tctgtggatg cgggtgatat cgcagcattt
    21001 aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct
    21061 gtggacctct ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt
    21121 tgcgttgggc attatcgcct ttatcgctgg cctggatatg tttaacggct taacccatat
    21181 gcaccgcccg gtggttctcg gcccgttggt cgggctggta cttggcgatc tgcataccgg
    21241 tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggccccgc tggcgggcgc
    21301 acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg
    21361 cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgttgcag tacagatggg
    21421 gattaccttc ctgttctcgg tgatgtccgg cgtgatgtcc cgctgcgacc ggatggcgga
    21481 gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcactgctgg cactcggcac
    21541 tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat
    21601 cattgatgtc ctgccgcaac gattaattga tggcctcggc gtcgcgggcg gcatcatgcc
    21661 agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt
    21721 catcctgggc tttgttgccg cagcctggct caagttaccg gtgctggcta ttgctgccgc
    21781 tgcgctggcg atggcgctga tcgacctgct gcgtaaatct cctgaaccga ctcaacctgc
    21841 ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagcg
    21901 aaaatgaaga aacactgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg
    21961 gcgcagagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgctccatg ctgttacaag
    22021 cgtctttcaa ctacgaacgt atgcaggctt ccggctggtt gtacggtctg ctgcctgctc
    22081 tgaaaaagat ccacactaat aaacgggacc tggcgcgcgc catgaagggg catatgggtt
    22141 tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt gcgatggagc
    22201 gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg
    22261 gcgggattgg cgatgcgatg ttctggctaa cgctactgcc gatttgtggc gggataggcg
    22321 caagcctcgc gctacaaggc tctattcttg gcgctgtcgt ctttattgtg ctgttcaacg
    22381 tggtgcacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg
    22441 ctattccact cattaaagca aataccaaaa aagtcggcca tgcggcgtct atcgttggga
    22501 tgacggtgat cggcgcgctg gtggcaacct atgttcgttt aagcaccacg ctggaaatca
    22561 cggcgggcga cgcagtggtt aagttacagg ctgatgttat cgacaaactg atgcctgcct
    22621 tcttaccgct ggtctacacc ctgaccatgt tctggctggt acgccgcggc tggagtccgc
    22681 tgcgcctgat tgcagtgaca gtggttctcg gcatcgtcgg taaattctgc catttccttt
    22741 aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg gatttgccag
    22801 cggcatggaa aaagcaatga agcaaatcct cggcgagcaa tcgcagttta tcgccatcga
    22861 ttttccagaa acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca
    22921 actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg
    22981 cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg gcacaaattt
    23041 gcaactatta ctggaaatgg tgctggaacg cgaagggtta agcggtgaag agttccgtgt
    23101 gcaggcgctg gagtgtggac accgtgggct aaccagtctg gtggacgaac ttggtcgctg
    23161 ccatgaggaa tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc cagaaggctg
    23221 ctgactgaag agggatggct cgatgaccat cagttgcgta ttgctgacgg tgtaatcgca
    23281 gcaatcgaac cgattccagt gggcgtgact gaacgcgatg cggaactgct ctgccccgct
    23341 tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat
    23401 gcgctcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc
    23461 accgtaaccg cgccgcttaa taccattcat gcggcgctga aacgtattgc tctacgctgc
    23521 cagcgcggtg ggcctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg
    23581 ccgcagaata aaggcgcaca tccgccggag ctgtttcgcg agctggaaat tgccgagctg
    23641 gatcaattga ttgccgtttc tcagcagacc ttacgcgtgg tagcgctggc accagaaaaa
    23701 gaaggggcat tgcaggccat ccgccatctt aaacagcaaa acgtacgggt gatgctgggg
    23761 catagcgcgg cgacctggca acaaacccgt gccgcgtttg atgctggtgc cgacggcctg
    23821 gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg
    23881 ggattaacgg acaagcgcgc ctggctggaa ctgatagccg atggtcatca tgtacatccg
    23941 gcggcaatgt cgctctgttg ttgctgtgcg aaagagagga tcgtactgat caccgacgcg
    24001 atgcaggcag ccgggatgcc ggatggtcac tatacgttat gtggtgaaga agtgcagatg
    24061 cacggtggca ttgtccgtac cgcgttcggt gggctggcgg gcagtacgct gtctgtcgat
    24121 gcggcagtgc gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc catccatatg
    24181 gcgtcgctgc atccggcgcg aatgctgggt gttgatggtg ttctgggatc gcttaaaccg
    24241 ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca aatctggatt
    24301 cagggtcaat tagcttcgtt ttgatggttt gctcctttat taggccttca cttcccccgt
    24361 aaggcctttc tttttctttc tttttgatct gtgcagcggt gtcggatgcg acgctaatgc
    24421 gccttatccg acctacggtt ggtgaccgta aggccggata aagtatttac gccgcatcca
    24481 gcaatccctt ttacttccct tgtcttttct ttcaacgatc acagatttcg ttttatttcc
    24541 tttttctcca ttgaactttc agtttctttt ctatagattt taaccaacga aagaaatcac
    24601 caagtgaaat gaaacgaaag gcaagcgaaa gcgacaacgc cgacgtcaag ttcatcagac
    24661 taaggattga gttatgccag aaaattacac ccctgctgct gccgcaaccg gtacatggac
    24721 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acattgacgc
    24781 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcaaat
    24841 catcctgacc ggagccggaa catcggcatt tatcggtgac atcatcgcgc cgtggctcgc
    24901 cagccatacc ggtaaaaact tcagcgccgt accaaccacc gatctggtca ccaatccaat
    24961 ggactacctg aacccagctc atccgctact gttgatctcc ttcggtcgct ccggcaacag
    25021 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtaccagaat gctatcacct
    25081 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgacaacgc
    25141 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
    25201 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
    25261 cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga
    25321 aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
    25381 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg acagcgggta aactggcggc
    25441 cttctatgat tccccgaccg gattccgtca tggcccgaaa tcgctggtcg atgacgaaac
    25501 gctggtagtg gtgtttgtct ccagtcaccc ttacacccgt cagtacgatc ttgacctgct
    25561 ggcagaactc cgccgtgaca accaggcaat gcgcgtgatc gccatcaccg ctgaaagcaa
    25621 cgacgttatc gccgctggcc cgcatatcat cctgccgccg tcccgtcact ttatcgacgt
    25681 tgaacaagca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
    25741 catgggcaat acgccggata ccccatcagc cagtggcacc gtaaaccgcg tggtgcaagg
    25801 cgtaactatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
    25861 atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgccttta acatccataa
    25921 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
    25981 cgccgggaca ccaggtacct ttaagcatat cgcgctggaa gagatctacg ccctgtgtag
    26041 cgcctattcc acgacctata acatgccgct ggcactgcat ctcgaccacc acgaatcgct
    26101 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagc gcgatgatcg acggcagcca
    26161 cttcccgttt gccgagaacg tgaagctggt gaaatcggtt gtcgacttct gccactcccg
    26221 ggattgcagc gtggaagcag aactgggccg cctcggcggt gttgaagatg acatgagcgt
    26281 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac
    26341 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
    26401 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
    26461 gcatggtgcc agcgatgtcc cggatgaatt tgtccgtcgc actattgaac tcggtgtaac
    26521 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
    26581 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
    26641 gaaagaagtg gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
    26701 atcattaatt ttatacgttt tttcagaatg cactgcccga ttaatatcgg gtagtacctt
    26761 aatggcaaag gagataacca aacattaata aatacatcag tacaatatcg caacaataat
    26821 atatttaaaa atattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa
    26881 atattctctt aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga
    26941 catccaccat cggtgcgaat ctgttggtag tcgtggatga tgttgtcgct aacgatgata
    27001 ttcaacagaa attaatgggt attaccgcag aaacctacgg ctttggcatt cgtttcttta
    27061 ctatcgaaaa aaccattaac gtcatcggca aagctgcacc acatcagaag atcttcctga
    27121 tttgccgtac gccacaaacg gtacgtaaat tggtagaagg tggtattgac ctgaaagatg
    27181 tcaacgtcgg caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg
    27241 tcgatgacca ggatctcacg gacttacgtt atattaaaca acgtggcgtg aatgttttca
    27301 ttcaggacgt tcctggcgat caaaaagaac aaatccctga ctaaatctga aatcgcctta
    27361 atattggttt gaggtaataa aaatgcatga aataacccta ctccagggat tatccctggc
    27421 ggcgttagtt ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc
    27481 gataatcgtt tgtaccctta caggtgctat tctcggtgat attcagactg gcttaattac
    27541 cggaggcctg acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc
    27601 caaccctatt atggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc
    27661 caaaacggca attggtcttg gcctgccgtt tagtttgtta atgcagtacg tcattctgtt
    27721 cttctattcc gctttctcat tatttatgac caaagccgat aaatgcgcga aagaggcgga
    27781 tacggcagcg ttttcccgac ttaactggac aacgatgctc atcgtcgctt cagcgtatgc
    27841 ggtgattgct ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc
    27901 gatgcccgcc tggctgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg
    27961 ttttggcttg ctgctgcgcg tgatgttcaa agcgcaatat atcccttacc tgatcgccgg
    28021 tttcctgttt gtttgctaca tccaggtcag caacctgttg ccggttgccg tgctgggcgc
    28081 aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc
    28141 cgttgccagt aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag
    28201 atatcacccg tctgggcttt cgttcgtcac tgctacaagc aagctttaac tacgaaagga
    28261 tgcaggcggg cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataagaacg
    28321 acaaaccggg cctaagcgcg gcgatgaaag ataacctcga atttattaat acccacccta
    28381 atctggtcgg atttctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg
    28441 acaccattaa aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga
    28501 ttttctggtt tactttgttg ccgattatgg cggggatttg ctcatcgttt gccagtcagg
    28561 ggaacctgtt gggaccgatt ttgtttttcg ccgtttacct gcttatcttt ttcctgcgcg
    28621 tcggctggac ccacgtcggt tattcagtcg gcgtgaaggc tattgataaa gtgcgagaga
    28681 actcgcagat gattgcccgt tcggcaacca tcctcgggat cacagtaatc ggcgggctga
    28741 tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg
    28801 tcgcgctgca gcaggatttc ttcgataaag tcttcccgaa tattttaccg atggcctaca
    28861 ccctgctgat gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg
    28921 tgacgtttgt tctttctatt gtttgttctg cattcggcat attgta
//
LOCUS       DAOFSV010000049        28522 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.049, whole genome shotgun sequence.
ACCESSION   DAOFSV010000049 DAOFSV010000000
VERSION     DAOFSV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.049"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            42..371
                     /locus_tag="QBH27_002254"
     CDS             42..371
                     /locus_tag="QBH27_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDQ7014558.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            371..1153
                     /gene="truC"
                     /locus_tag="QBH27_002255"
     CDS             371..1153
                     /gene="truC"
                     /locus_tag="QBH27_002255"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDQ7014559.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            1171..1620
                     /locus_tag="QBH27_002256"
     CDS             1171..1620
                     /locus_tag="QBH27_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDQ7014560.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            2054..3406
                     /gene="gudP"
                     /locus_tag="QBH27_002257"
     CDS             2054..3406
                     /gene="gudP"
                     /locus_tag="QBH27_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDQ7014561.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            3408..4748
                     /locus_tag="QBH27_002258"
     CDS             3408..4748
                     /locus_tag="QBH27_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDQ7014562.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVGAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            4769..6109
                     /gene="gudD"
                     /locus_tag="QBH27_002259"
     CDS             4769..6109
                     /gene="gudD"
                     /locus_tag="QBH27_002259"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDQ7014563.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(6340..9096)
                     /gene="barA"
                     /locus_tag="QBH27_002260"
     CDS             complement(6340..9096)
                     /gene="barA"
                     /locus_tag="QBH27_002260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDQ7014564.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFYHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            9153..10454
                     /gene="rlmD"
                     /locus_tag="QBH27_002261"
     CDS             9153..10454
                     /gene="rlmD"
                     /locus_tag="QBH27_002261"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDQ7014565.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            10502..12736
                     /locus_tag="QBH27_002262"
     CDS             10502..12736
                     /locus_tag="QBH27_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDQ7014566.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            12832..13623
                     /gene="mazG"
                     /locus_tag="QBH27_002263"
     CDS             12832..13623
                     /gene="mazG"
                     /locus_tag="QBH27_002263"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDQ7014567.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQIAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            13851..15488
                     /gene="pyrG"
                     /locus_tag="QBH27_002264"
     CDS             13851..15488
                     /gene="pyrG"
                     /locus_tag="QBH27_002264"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDQ7014568.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            15576..16874
                     /gene="eno"
                     /locus_tag="QBH27_002265"
     CDS             15576..16874
                     /gene="eno"
                     /locus_tag="QBH27_002265"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDQ7014569.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(16934..17806)
                     /locus_tag="QBH27_002266"
     CDS             complement(16934..17806)
                     /locus_tag="QBH27_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDQ7014570.1"
                     /translation="MRYFILIFTLVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     TRKLESIFNNTQVQIAVLIIPTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQQLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(17820..17961)
                     /locus_tag="QBH27_002267"
                     /pseudo
     CDS             complement(17820..17961)
                     /locus_tag="QBH27_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18100..18771
                     /gene="queE"
                     /locus_tag="QBH27_002268"
     CDS             18100..18771
                     /gene="queE"
                     /locus_tag="QBH27_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDQ7014571.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            complement(18856..19863)
                     /locus_tag="QBH27_002269"
     CDS             complement(18856..19863)
                     /locus_tag="QBH27_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ7014572.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(19889..21298)
                     /locus_tag="QBH27_002270"
     CDS             complement(19889..21298)
                     /locus_tag="QBH27_002270"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDQ7014573.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            complement(21298..22668)
                     /locus_tag="QBH27_002271"
     CDS             complement(21298..22668)
                     /locus_tag="QBH27_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDQ7014574.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            complement(22729..24246)
                     /locus_tag="QBH27_002272"
     CDS             complement(22729..24246)
                     /locus_tag="QBH27_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDQ7014575.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNETFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            complement(24414..25334)
                     /locus_tag="QBH27_002273"
     CDS             complement(24414..25334)
                     /locus_tag="QBH27_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDQ7014576.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISDESQVQQGAYSLVQRYPLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            complement(25499..26977)
                     /locus_tag="QBH27_002274"
     CDS             complement(25499..26977)
                     /locus_tag="QBH27_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ7014577.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     ATVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(27004..28281)
                     /locus_tag="QBH27_002275"
     CDS             complement(27004..28281)
                     /locus_tag="QBH27_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7014578.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
BASE COUNT         7259 a         6879 c         7247 g         7137 t
ORIGIN      
        1 ctgctatcct tcgcggcagt ttttcttatt gaggttgctt tatgaccact catgaccgcg
       61 ttcgtctcca gttgcaggcg cttgaagcgt tactgcgtga acatcagcac tggcgaaacg
      121 atgaacccca gccgcatcaa tttaatagta cccaaccgtt ctttatggac accatggaac
      181 cactggagtg gttgcagtgg gtgctgatcc cgcgaatgca cgatttgtta gataacaacc
      241 agccgttacc gggcgctttt gctgttgcgc cctattatga aatggcgctg gcgactgatc
      301 atccgcaacg cgcgcttatt ctggctgagc tggagaaact ggatgctctt tttgcggatg
      361 atgcgagcta atgctggaaa tactctatca ggatgaatgg ctggttgcgg taaataaacc
      421 ctccggctgg ctggttcacc gcagctggct ggatcgcgac gagaaagtag tggtcatgca
      481 aaccgtgcgt gaccagatag gccagcatgt ttttactgct catcgtctgg accgaccaac
      541 ttctggtgtg ttgttgatgg gattatccag cgaagccgga cggctgctgg cacaacagtt
      601 tgaacagcac caaatccaga aacgttacca tgcgattgtg cgtggctggt tgatggaaga
      661 agcggtgctg gattatccac tggtggaaga actggacaaa atcgctgata aatttgcccg
      721 cgaagataaa ggcccgcagc cagcagtgac gcattatcgc ggtctggcga ccgtagaaat
      781 gccggtagcg actggacgtt acccgaccac gcgctacggc ctggtggaac tggagccgaa
      841 aactggacgc aagcaccagc ttcgtcgcca tcttgcccat ttgcggcacc cgattattgg
      901 cgacagcaaa catggtgatt tacgccagaa tcgcagcggt gctgagcatt ttggccttca
      961 gcggttaatg ctgcatgcca gtcagttgtc attgacgcat ccttttactg gcgagccgct
     1021 ggctattcac gcgggcctgg acgacacctg gatgcaggcg ttatcacaat ttggctggcg
     1081 gggtctgctt cctgaaaatg aaagggttga gttcagtgcg ccatcgggtc aggatggaga
     1141 gataagttcc taatccaggg agaaaacggt atggcggaaa ttggtatttt tgtcggcact
     1201 atgtacggaa attcgctgtt agtggccgaa gaagcggaag cgattctgac cgcgcagggc
     1261 cacaaagcaa cggtatttga agatcctgaa ttaagcgact ggctgcccta tcaggataag
     1321 tatgttctgg tggttacgtc cacgaccggg cagggcgacc ttcctgatag cattgtgccg
     1381 ctctttcagg gaattaaaga taccctgggc ttccagccga atctgcgtta tggcgtgatt
     1441 gcgctcggcg acagtagtta tgtgaatttc tgcaatggcg gcaaacagtt cgatgccttg
     1501 ttgcaggaac agagcgctca gcgggttggc gaaatgctgt tgattgacgc cagtgagaac
     1561 ccggaaccgg aaacggaatc aaatccgtgg gttgaacatt ggggcacgat gttgtcctga
     1621 tatgttcagc gagcggtaaa tgccgtttta gcggtgctga atcgaatctt ttcaggcaaa
     1681 tgccagtaaa aactgcttca tagcgcggat ttttactggc gtttgcctgg agtcaagcga
     1741 tccatttcat actcttcttt atttcttcgt tttaaccctt cctttcttgt tcttgttttc
     1801 atttccgtga agtggattcc accgtccagg gctaatgcca aaatcgggcc tcattgaacg
     1861 cattaatgtt gtgttgttgc acggtgagcc gctatggcgc gctttttata ctgctattgc
     1921 cagatataaa cacgcgccgt attcggcgaa cgacctataa aaacggcaaa aacaccctac
     1981 gtcacctctg atttcctggc gatgtcgcag tccagagtga gcgtggctaa cgcgaatttt
     2041 caggagtgca acaatgagtt ctttaagtca ggctgcgagc agtgtggaaa aacgcacgaa
     2101 tgctcgttac tggatagtgg tgatgttgtt tatcgtcaca tccttcaact acggcgaccg
     2161 cgctacgctt tctattgccg gttcggaaat ggccaaagat atcggccttg atcccgtggg
     2221 aatgggctat gtgttctctg ccttctcatg ggcttatgtt atcgggcaga tccctggtgg
     2281 ctggttgctg gatcgttttg gttcaaaacg cgtctacttc tggtcgatct ttatctggtc
     2341 gatgtttacc ttgctgcaag gcttcgtcga tatctttagt ggattcggca ttatcgtggc
     2401 cctgtttaca ctgcgcttcc tggtcgggct tgctgaagcg ccatctttcc ctggcaacag
     2461 ccgcattgtt gcggcctggt ttccggcgca ggaaagggga acggcggtgt cgatttttaa
     2521 ctccgcccaa tacttcgcaa cggttatctt cgcgccgatt atgggctggc tgactcatga
     2581 agtgggctgg tcacacgtct tcttctttat gggcggtctg ggcattgtca tcagctttat
     2641 ctggttgaaa gtcatccacg agccaaatca acatccgggc gtaaatcaga aagagctgga
     2701 gtacatcgcc gcgggtggcg cgctgatcaa tatggatcag caaaacacca aagttaaagt
     2761 gccgttcagc gtgaagtggg ggcagatcaa acagctgctc gggtcacgga tgatgatcgg
     2821 cgtttatatc ggtcagtact gtatcaacgc cctgacttac tttttcatta cctggttccc
     2881 ggtttatctg gtgcaggcgc gcgggatgtc gattctgaaa gcgggctttg tggcttccgt
     2941 tccggcggtt tgcggtttta tcggcggtgt gttggggggg attatttccg actggctgat
     3001 gcgtcgcacg ggttcactga acattgcgcg taaaacaccg atcgtgatgg gcatgttgtt
     3061 gtcgatggtg atggtgttct gcaactacgt caacgttgag tggatgatca tcggctttat
     3121 ggcgctggcc ttcttcggta aggggatcgg tgcgctgggt tgggctgtaa tggctgatac
     3181 tgcgccaaaa gagatcagcg gtctttccgg tggcctgttc aacatgtttg gtaacatttc
     3241 tggcatcgtc acgccaatcg caattggtta tatcgttggc acgactggct cctttaatgg
     3301 ggcgctgatt tatgttggtg ttcatgcctt aatcgcggta ctcagctacc tggtgctggt
     3361 gggcgatatc aagcgtattg agctgaaacc tgtcgcgggg caataagatg acgacacaat
     3421 ccagtcctgt tattactgat atgaaagtca ttccggtcgc cggacatgac agcatgttgc
     3481 ttaatattgg tggtgcacat aacgcatatt tcacccgcaa tattgtggta ctcaccgata
     3541 atgccgggca taccggcatt ggtgaagcgc cgggcggaga ggtgatttat caaacactgg
     3601 tcggggctat tccgatggtg ctgggccagg aagttgcgcg cctgaataaa gtggttcagc
     3661 aggtgcataa aggtaatcag gcagccgatt ttgatacctt cggcaaaggt gcctggactt
     3721 ttgaattgcg cgttaatgcc gtagcggcgc tggaagccgc cttgcttgac ctgttaggtc
     3781 aggcgctgaa tgttccggtc tgcgaactgt tagggccagg caagcaacgt gatgctatta
     3841 ccgttctcgg ttatctgttt tatatcggtg atcggaccaa aaccgatctt ccttatctgg
     3901 aaaatacgcc gggcaaccat gagtggtatc agttgcggca tcagaaagcg atgaacagcg
     3961 aagccgttgt gcgtctggcg gaagcctcac aggatcgcta tggctttaaa gatttcaaac
     4021 ttaagggtgg cgtgttacct ggcgagcaag aaatcgacac tgttcgtgca ttgaagaaac
     4081 gcttcccgga tgcgcggatt accgttgatc ccaatggtgc ctggctgctt gatgaagcca
     4141 tttctctgtg caaagggctg aatgatgttc ttacctatgc ggaagacccg tgcggcgctg
     4201 agcagggttt ctccggtcgt gaagtgatgg cggagtttcg acgggcgacc ggcttgcccg
     4261 tcgcgaccaa catgattgcc accaactggc gcgaaatggg tcatgcggtg atgctcaatg
     4321 cggtagatat tccacttgcc gatccgcact tctggacgct ttccggtgca gtccgcgtgg
     4381 cgcaactttg cgacgactgg gggctgacct ggggctgcca ttctaataac cactttgata
     4441 tctctctggc gatgtttacc catgtgggcg cggcggcacc gggtaatcct accgctatcg
     4501 atacccactg gatttggcag gagggcgatt gtcgtctgac caaaaatccg ctggagatta
     4561 aaaacggaaa aattgccgtt cctgatgcgc ccggtctggg cgtggaactg gactgggaac
     4621 aggtacaaaa ggcacatgag gcctataaac gtctgcctgg cggtgcgcgt aacgacgcag
     4681 gcccgatgca gtacctgatc cccggctgga cctttgaccg taaacgcccc gttttcggcc
     4741 gtcattgatt ctgaaaaagg acataaatat gagttctcaa tttacgacgc ctgttgttac
     4801 tgaaatgcag gtcatcccgg tggcgggtca tgacagtatg ctgatgaatc tgagtggtgc
     4861 acatgcaccg ttcttcacgc gtaatattgt gattatcaaa gataattccg gtcacactgg
     4921 cgtaggggaa attcccggcg gcgagaaaat ccgtaaaacg ctggaagatg cgattccgct
     4981 ggtggtgggt aaaacgctgg gtgaatacaa aaacgttctg acgctggtgc gtaatacgtt
     5041 tgccgatcgt gatgccggtg ggcgcggttt gcagacattt gatttgcgta ccactattca
     5101 tgtagttacc gggatagaag cggcaatgct ggatctgttg gggcagcatc tgggggtaaa
     5161 cgtggcatcg ctgctgggcg atggccaaca gcgtagcgaa gtcgaaatgc tcggttatct
     5221 gttcttcgtc ggtaatcgca aagccacacc gctgccgtat caaagccagc cggatgatca
     5281 atgtgactgg tatcgcctgc gtcacgaaga agcgatgacg ccggatgcgg tggtgcgcct
     5341 ggcggaagcg gcatatgaaa aatatggctt caacgatttc aaactgaagg gcggtgtact
     5401 ggccggggaa gaagaagcag aatctattgt ggcactggcg caacgtttcc cgcaggcgcg
     5461 tattacgctc gatcctaacg gtgcctggtc gctgaacgaa gcgattaaaa tcggtacata
     5521 cctgaaaggt tcgctggctt atgcagaaga tccgtgtggt gcggagcaag gtttctccgg
     5581 gcgtgaagtg atggcagagt tccgtcgcgc aactggcctg ccgaccgcaa ccaatatgat
     5641 cgccaccgac tggcggcaga tgggccatac gctctcgttg caatccgttg atatcccgct
     5701 tgcggatccg catttctgga cgatgcaagg ttcggtacgt gtggcgcaaa tgtgtcatga
     5761 atttggcctg acctggggtt cacactccaa caaccacttc gatatttccc tggcgatgtt
     5821 tacccatgtt gccgctgccg caccgggtaa aatcaccgcg attgatacgc actggatctg
     5881 gcaggaaggt aatcagcgcc tgaccaaaga accgttagag atcaaaggcg gtctggttca
     5941 ggtgccagaa aaaccggggc tgggtgtaga aatcgatatg gatcaagtga tgaaagcgca
     6001 cgagctgtat cagaaacacg ggcttggcgc gcgtgacgat gcgatgggaa tgcagtacct
     6061 gatccccggc tggacgttcg ataacaagcg tccgtgcatg gtgcgttaag acagaaaacc
     6121 aaaaccggct ctatctggag ccggtttgag actgatgcca aacgcaaaat tgcctgttgc
     6181 gctacgtatc agggctacat cattttttaa ttcattgaat ttaattggtt ttgcaggccg
     6241 gataaggcgt taacgccgca tccggcataa acacaggcac ttgtcaccaa tctgaaacca
     6301 gcgtcataaa aatccggttg ctactcgaca agacgtccat tacccgagaa ttttgctggc
     6361 ttcgcgcgct acattatcca tctcgtccaa cagttccagc agctccggct ccaaatcttc
     6421 ttctttagta ccgctacgta gctgttgttc gatcagttgg cagagattct tcatacgcgg
     6481 tacaccgcta tagccgcaac tgccatgcag tttatgaatc aaatcaacca ggccttccgg
     6541 gttttctcca accagctgtt cctcaacttt gttgcgaact tcaggcagga aatcgagtaa
     6601 catttgcagc atatcgcgcg ctaaatcggt ttttcctgct gcctggcgta gtgccagttg
     6661 ccagtcgagg gtcgcattcg ggttgaccac aatttcgttg acttcgggcg tcacgacgcg
     6721 agaggaaata ccgctgccag gcttgtagcg caacagcaaa ttatgcaatc gctcttcttc
     6781 gatcggtttg gcgagataat cactcatccc cgcgccaagc agtttctctt tttgcccggc
     6841 catcgcatgc gccgttaccg cgataaccgg cgtttgctgc tgatgcggga gctggtggat
     6901 gagctcacat gcccgaatgc cgtccatgtc cggcatttga atatccatta agattaaatc
     6961 gaacggcatc tgtttcgccc gttcaactgc ctgatgcccg ctatcgcaaa gttccacatg
     7021 ttgcaccata tcttccagca atgcgccgat aagtttcagg ttagcggggt tgtcatcaac
     7081 cgccatgact gtcattgcca gcttactttc atcggttaca ggcaaaagcg tgttttgttt
     7141 gtgatgataa aattccgtca gggcaggcaa caggcgcgta ggtgttaatg gtttcagcag
     7201 acacgcgccg ataccatctt gcttaagttt ttcagcattg acttgtgcat ggcaaggaag
     7261 tgccagcatc aggaaatcgg tcatcgatac cgctttcgct aatcgctcat gttgcattgt
     7321 tagcggctcg cggaaggtca ccgcgatgcc taacaacatc atgtcgtaat gcgcgggagg
     7381 cagcgcggag aacgttgggc tataaaccac ttccagcggc gtttcactta aaatatccag
     7441 cgtgcattgt gctgctgcgg agtttggttc gacataggcc aggcgcttac cagcgaggca
     7501 ctgggtggat ggcccttcga taataatgtt cgggttcaga tcgagattaa tgtggaacca
     7561 gaaagttgaa ccacgattcg gcaggctatg gaacgaaata tcaccgccca tttcattaac
     7621 cagtttttgt gtaatcacca gccccagccc ggtgccacca tgacggcggg aaatactggc
     7681 atcagcctgt ctgaaggcct ggaataagcg cgattgatcg cgttcaggaa taccaatgcc
     7741 ggtatcccga atctgcactt caatttgcac tttggtatta ctcagcgcac gtttttccac
     7801 cagaatatcg atgttgccat tctcggtgaa tttaatcgcg ttccccacca ggttagtgat
     7861 gatttgctgt aaacgtagcg ggtcgccaat cacgttatcg gggacgtcgc tcttaatatt
     7921 gagcgtcaat tccagccctt tatcgtggga agagtgtgcc agaagggtaa cgacttcatc
     7981 cagcgtgctg cgcagtggga acggaatact ttccagaatc agcttaccgg cttccagttt
     8041 cgagaagtcg agaacatcat taataattgc cagcaaatta tttgccgaac gttcaatcgt
     8101 attcaggtga tcgcgctgcg ttggtgttaa ttctgttttc agcgtcaggc gggtaaagcc
     8161 aataacacca ttcagtggtg tacgcagctc gtgtgacata tttgccagaa actcggattt
     8221 aatacgcgcc gcttcctggg cgcgcttttt cgccagatcc aactcaacgt tctgaatctc
     8281 catttgctcc agcgtctcac gcagatcgga cgtcgcctgg tcgatattgt gttgcatctc
     8341 ttcgtgataa gccgccagcg acattgccat cgagttgata ccgtttttca gcatatccag
     8401 ctcgccgagc ataaatcctt ccacccggct gtcgagttgc ccgcgacgga tgcggtcgac
     8461 ggtattcacc atgttgcgaa tcggaccggt tacatcgcgc attaagcgcc agccaaaaat
     8521 aagcgcaata ccgatacaaa acagcatcat cacgctggaa ataaagatct ctttatattg
     8581 ctgcaagcga accgatttaa gatccagctc aagcgcaata tatcccaaca tattttgact
     8641 atttttggca tcgctactgg gcgattcgtc gggggagtaa ctctcagaaa taatcggcgt
     8701 gcggaggatc ataatatcgc cgtcacgagt gacagtgagc tggcgaggaa acggcacgtt
     8761 gctgccgagc tgcattgatg agggatcaag atgaaaattg gaggtgacaa agagtcggtt
     8821 attttcatca taaaccgaaa tcgcgcgaac aatatcagaa tggcgacgat gcagtacgct
     8881 tattaactga ccgatagatt cgcgattttg cagactcatg ccatattcag tagaaactgc
     8941 aagcggctca ataatgctgg caccggcatc ttccagttga cgctgcaagt cgttatagcg
     9001 atgcacgacg aaaaagatac tcagcaataa accaataagg acggtcggtg ccaggatcag
     9061 aatcatcatg cgtgcgcgca ggctgtagtt ggtcatggag ttccgttatg ggacaattaa
     9121 ggtcacactg ttaaattgag aaagtctcgc caatggcgca attctactct gcaaaacgac
     9181 gcacgacgac gcgtcagatc ataaccgttt cagtcaacga cctcgactct tttggtcagg
     9241 gtgtggcgcg acataacggc aaaacattat ttatccccgg attattgccg caggaaaacg
     9301 cggaagttac tgttactgaa gataaaaaac agtatgcccg cgctaaagtc gtacgccggt
     9361 taagcgatag cccggaacgc gaaacgccac gctgtcctca ttttggcgta tgcggcggct
     9421 gtcagcaaca acacgccagc gtggatttac agcagcgaag caaaagtgcg gcactcgccc
     9481 gattaatgaa acacgaagtc tctgaagtga tcgccgatgt tccttggggc tatcgccgtc
     9541 gcgcgcgttt aagtctgaac tacttaccga aaacacagca acttcagatg gggtttcgca
     9601 aagcgggctc cagtgacatt gtcgacgtta aacaatgccc cattttagtg ccccaactag
     9661 aagcattgct gcccaaagtc agggcatgcc tgggcagctt acaagctatg cgccatcttg
     9721 gtcatgttga actggtacag gcaaccagcg gcacgctaat gattttgcgc cataccgcac
     9781 cgctaagttc ggcagatcgc gaaaaactgg aatgcttttc gcattctgaa ggcctggatc
     9841 tgtatctcgc ccccgatagt gagatactcg aaaccgtctc tggtgagatg ccctggtatg
     9901 actcaaatgg gttgcgctta acttttagcc cgcgcgattt tattcaggtc aatgcgggtg
     9961 tgaaccaaaa aatggtagcg cgtgcgttgg aatggctgga tgtggaacct gaagatcgcg
    10021 tactggatct gttctgcggt atgggcaact ttacactgcc attggcgaca caagctgcca
    10081 gtgtggtcgg tgtagaaggt gttccggcgc tggtggaaaa aggccagcag aatgcgcgtc
    10141 ttaatggctt acagaatgtg acgttttatc acgaaaatct tgaagaagat gtcacaaagc
    10201 agccgtgggc gaaaaacggc ttcgataaag tgttgttgga cccggcgcga gcaggtgccg
    10261 caggtgttat gcagcaaatt ataaaactgg aacctattcg tatagtttat gtatcctgta
    10321 accctgcaac gctggctcgg gatagcgaag cgttattaaa agcaggatat accattgcgc
    10381 gactggcgat gctggatatg ttcccacaca cgggacatct ggaatcgatg gtacttttct
    10441 cgcgcgttaa atagttacga tttgccgatt tcggcaggtc tggtccctaa aggagaggac
    10501 gatggttgcg gtaagaagtg cacatatcaa taaggctggt gaatttgatc cggaaaaatg
    10561 gatcgcaagt ctgggtatta ccagccagaa gtcgtgtgag tgcttagccg aaacctgggc
    10621 gtattgtctg caacagacgc aggggcatcc ggatgccagt ctgttattgt ggcgtggtgt
    10681 tgagatggtg gagatcctct cgacattaag tatggacatt gacacgctgc gggcggcgct
    10741 gctattccct ctggctgatg ccaacgtagt cagcgaagat gtgctgcgcg agagcgtcgg
    10801 taagtcggtc gttaacctta ttcacggcgt gcgtgatatg gcggcgatcc gccagctgaa
    10861 agcgacgcac actgattctg tttcctccga acaggtcgat aacgttcgcc ggatgttatt
    10921 ggcgatggtc gatgattttc gctgcgtagt catcaaactg gcggagcgta ttgctcatct
    10981 gcgcgaagta aaagatgcgc cggaagatga acgtgtactg gcggcaaaag agtgcaccaa
    11041 tatctacgcg ccgttggcga accgtcttgg gattgggcaa ctgaaatggg agctggaaga
    11101 ttactgcttc cgttatctcc acccgaccga atacaaacgc atcgcaaaac tgttgcatga
    11161 acgccgtctc gaccgcgaac actatatcga agagtttgtc ggccatctgc gcgctgagat
    11221 gaaagctgaa ggcgttaaag ccgaagtgta tggccgtccg aaacacatct acagcatctg
    11281 gcgcaaaatg cagaaaaaga acctcgcctt cgatgagctg tttgatgtgc gtgcggtacg
    11341 tattgtcgcc gagcgtttac aggattgtta tgccgcactg gggatagtgc acactcacta
    11401 tcgccacctg ccggatgagt ttgacgatta cgtcgctaac ccgaaaccaa acggttatca
    11461 gtctattcat accgtggttc tggggccggg tggcaaaacc gttgagatcc aaatccgcac
    11521 caaacagatg catgaagatg cagagttggg tgttgctgcg cactggaaat ataaagaggg
    11581 cgcggctgct ggcggcgcac gttcgggaca tgaagaccgg attgcctggc tgcgtaaact
    11641 gattgcgtgg caggaagaga tggctgattc cggcgaaatg ctcgacgaag tacgtagtca
    11701 ggtctttgac gaccgagtgt acgtctttac gccgaaaggt gatgtcgttg atttgcctgc
    11761 gggatcaacg ccgctggact tcgcttacca catccacagt gatgtcggac accgctgcat
    11821 cggggcaaaa attggcgggc gcattgtgcc gttcacctac cagctgcaga tgggcgacca
    11881 gattgaaatt atcacccaga aacagccgaa ccccagccgt gactggttaa acccaaacct
    11941 cggttacgtc acaaccagcc gtgggcgttc gaaaattcac gcctggttcc gtaaacagga
    12001 ccgtgacaaa aacattctgg ctgggcggca aatccttgac gacgagctgg aacatctggg
    12061 gatcagcctg aaagaagcag aaaaacatct gctgccgcgt tacaacttca atgatgtcga
    12121 cgagttgctg gcggcgattg gtggcgggga tatccgtctc aatcagatgg tgaacttcct
    12181 gcaatcgcaa tttaataagc cgagtgccga agagcaggac gccgccgcgc tgaaacagct
    12241 tcagcaaaaa agctacacgc cgcaaaaccg cagtaaagat aacggtcgtg tggtggtaga
    12301 aggggtcggt aacctgatgc accacatcgc gcgctgctgc cagccgattc ctggagatga
    12361 gattgtcggc ttcattaccc aggggcgcgg tatttcagta caccgcgccg attgcgaaca
    12421 attggcggaa ctgcgctccc atgcgccaga acgcattgtt gacgcggtat ggggcgagag
    12481 ctactccgcc ggatattcgc tggtggtccg cgtggtggct aatgatcgta gtgggttgtt
    12541 acgtgatatc accaccattc tcgccaacga gaaggtgaac gtacttggcg ttgccagccg
    12601 tagcgacacc aaacagcaac tggcgaccat cgacatgacc attgagattt acaacctgca
    12661 agtgctgggt cgcgtgctgg gtaaactcaa ccaggtaccg gatgttatcg acgcgcgtcg
    12721 gttgcacggg agttaatctt acatagctgg atatggcgtc agccgtatcc agcgatatct
    12781 tttactcgaa ggtattacac ctgacggacc cgcaattttc aggataattc aatgaatcaa
    12841 atcgaccgtt tgctcaccat catgcagcgc ctgcgcgacc cggaaaacgg ctgcccgtgg
    12901 gataaagagc agacatttgc caccattgcg ccttacaccc ttgaagaaac ctacgaggtg
    12961 ctggacgcca tcgcccgtga agattttgac gatctgcgcg gtgaactggg cgatctgctg
    13021 ttccaggtgg tgttttacgc acaaatagct caggaagaag ggcgctttga ctttaatgat
    13081 atttgcgctg ctattagcga taaattagag cgtcgccatc cgcatgtttt tgctgacagt
    13141 tctgccgaaa atagtagtga agtgcttgcc cgttgggagc aaatcaaaac cgaagagcgc
    13201 gcgcagaaag cgcagcattc ggcgctggac gatattcctc gtagtttacc ggctttaatg
    13261 cgtgcgcaaa aaatccagaa acgttgcgcc aacgttggtt tcgactggac gacgcttggt
    13321 ccggtagtcg ataaagtcta cgaagagatc gacgaggtga tgtacgaggc gcggcaggct
    13381 gttgtcgacc aggctaaact ggaggaggaa atgggtgacc tgctgtttgc cacggtcaat
    13441 ctggctcgtc atttagggac aaaagcggaa atcgcattgc aaaaagcgaa cgaaaaattc
    13501 gagcgtcgtt ttcgcgaagt ggagcgtatt gttgccgcgc gtggactgga aatgacaggt
    13561 gttgacctcg aaacaatgga agaagtctgg caacaggtaa aacggcagga aattgatctc
    13621 taagggaatt acgtggtcaa gcgcgatttg tgtcattttt taaatgacaa gcgcttgatt
    13681 tgcgtcaaaa acatttaccc caaaggggct attttctcac tcctgatttc aatagtgcgc
    13741 tggcgaagag gagggataat gaaagtttgt ggcacaggtc atgttcgggt atactgcttt
    13801 cccgtcctgg ttattccatc gtcttttcaa cctaacttct caggttcagc atgacaacga
    13861 actatatttt tgtgaccggc ggggtcgtat cctctctggg taaaggcatt gccgcagcct
    13921 ccctcgcagc cattcttgaa gcccgtggcc tcaatgtgac catcatgaaa ctggatccgt
    13981 acatcaacgt cgatcccggt actatgagcc caatccaaca cggggaagtg ttcgttactg
    14041 aagacggcgc tgaaaccgac ctggacctgg ggcactacga gcgtttcatt cgtaccaaaa
    14101 tgagccgccg caacaacttc accacgggtc gtatctactc tgacgttctg cgtaaagaac
    14161 gccgcggtga ctatctcggc gcaaccgtac aggttattcc gcacatcact aacgctatca
    14221 aagagcgcgt gctggaaggc ggcgaaggtc atgacgtagt actggtcgaa atcggcggta
    14281 ctgtcggtga tatcgaatcc ctgccgttcc tcgaagcgat tcgccagatg gctgttgaaa
    14341 ttggccgtga gcacaccctg tttatgcacc tgacgctggt gccgtacatg gcagcgtcag
    14401 gtgaagtcaa aaccaaaccg actcagcact ctgtaaaaga gctgctttca atcggtatcc
    14461 agcctgatat cctgatttgt cgttcagatc gcgccgttcc agcgaacgaa cgtgcgaaga
    14521 ttgcattgtt ctgtaatgtt ccggaaaaag cggttatttc tctgaaagac gtcgattcca
    14581 tctataaaat tccgggcctg ttgaaatctc aggggctgga cgattatatt tgtaaacgat
    14641 tcagcttaaa ctgcccggaa gcgaatctgt ccgaatggga acaggttatc ttcgaagaag
    14701 cgaatccggt aagtgaagtc accattggta tggtcggcaa atacattgag ctgccggatg
    14761 catacaaatc cgtgatcgaa gcactgaaac acggtgggct gaagaatcgt gtcagcgtca
    14821 acatcaaact gatcgattca caagatgttg aaacgcgcgg cgttgaaatc ctcaaaggtc
    14881 tggacgcaat cctcgtacct ggcggtttcg gctatcgtgg cgtagaaggc atgattacga
    14941 ccgcgcgttt tgcgcgtgag aacaatattc cttatctggg catttgcctg ggtatgcagg
    15001 tggcgttaat tgattacgct cgccatgttg ccaacatgga gaacgccaac tctacggaat
    15061 ttgtgccaga ctgtaagtac ccggttgtgg cgctgattac cgagtggcgc gatgaaaacg
    15121 gcaacgttga agttcgtagc gagaagagcg atctcggcgg taccatgcgt ctcggcgcac
    15181 agcagtgcca gttggttgac gatagcctgg ttcgccagct gtacaatgcg ccgacaattg
    15241 ttgagcgtca tcgtcaccgt tacgaagtca acaacatgct gttgaaacag attgaagatg
    15301 caggtctgcg cgttgcgggc cgttccgggg atgatcagtt ggtcgagatc atcgaagttc
    15361 cgaatcatcc gtggttcgtg gcttgccagt tccatccgga gtttacttct actccacgtg
    15421 atggtcaccc gctgtttgct ggctttgtaa aagccgccag cgagttccag aaacgtcagg
    15481 cgaagtaagt aaaaaagtta gaacggcaac gcgtaccctg ggtacgcgtt gtttgtctgg
    15541 agtttcagtt taactagtga cttgaggaaa acctaatgtc caaaatcgta aaaatcatcg
    15601 gtcgtgaaat catcgactcc cgtggtaacc cgactgttga agccgaagta catctggagg
    15661 gtggtttcgt cggtatggca gctgctccgt caggtgcttc tactggttcc cgtgaagctc
    15721 tggaactgcg cgatggcgac aaatcccgtt tcctgggcaa aggcgtaacc aaagctgttg
    15781 ctgcggttaa cggcccgatc gctcaggcgc tgattggcaa agatgctaaa gatcaggctg
    15841 gcattgacaa gatcatgatc gacctggacg gcactgaaaa caaatccaaa ttcggcgcaa
    15901 acgcaatcct ggctgtatct ctggctaacg ccaaagctgc tgcagctgct aaaggtatgc
    15961 cgctgtacga gcacatcgct gaactgaacg gcactccggg caaatactct atgccggttc
    16021 cgatgatgaa catcatcaac ggtggtgagc acgctgacaa caacgttgat atccaggaat
    16081 tcatgattca gccggttggc gcgaaaactg tgaaagaagc catccgcatg ggttctgaag
    16141 ttttccatca cctggcaaaa gttctgaaag cgaaaggcat gaacactgct gttggtgacg
    16201 aaggtggcta tgcgccgaac ctgggttcca acgctgaagc tctggctgtt atcgctgaag
    16261 ctgttaaagc tgctggttat gaactgggca aagacatcac tctggcgatg gactgcgcag
    16321 cttctgaatt ctacaaagac ggtaaatacg ttctggctgg cgaaggcaac aaagcattca
    16381 cctctgaaga attcactcac ttcctggaag aactgaccaa acagtatccg atcgtttcca
    16441 tcgaagacgg tctggacgaa tctgactggg acggtttcgc ataccaaacc aaagttctgg
    16501 gcgacaaaat ccagctggtt ggtgacgacc tgttcgtaac caacaccaag atcctgaaag
    16561 aaggtatcga aaaaggtatc gctaactcca tcctgatcaa attcaaccag atcggttctc
    16621 tgaccgaaac tctggctgca atcaagatgg cgaaagacgc tggctacact gcagttatct
    16681 ctcaccgttc tggcgaaact gaagacgcta ccatcgctga cctggctgtt ggtactgctg
    16741 caggccagat caaaacgggt tctatgagcc gttctgaccg tgttgctaaa tacaaccagc
    16801 tgattcgtat cgaagaagct ctgggcgaaa aagcaccgta caacggtcgt aaagagatca
    16861 aaggccaggc ataagtctga ctttatctga tttaaaaatg ccagccttcg ggctggcgtt
    16921 ttttatggtt gagttaccaa cgaccggacg cgccgccacc gccagaagaa ccgcccccgc
    16981 cgctaaatcc gccgccactt gagccgccac cggaaccgct gttgtctgaa tgataacttc
    17041 taccagatgc ttttttctga agtacacata aacaggtaaa gaccataatg gtggtgaaag
    17101 taaaaaagaa gaaaaataca gcaatggaaa aagttttatc ggagaacagg aataaatcta
    17161 aaagatagat tgctgaaata cagacactgc ttttacatgc gcgacaaaaa ttactgcctt
    17221 gatgaaaaaa ccagaaaggg aagaacatca ctgcaaatac ccaaaaaata gcaaagtagt
    17281 aatgatcgct ggaagatgct gattcacttt ctgaaggatt aaccgaatat tggtgttgtg
    17341 aagtgagtcg gttattcaaa gcgattatag ctaactctaa tcctttagct aattgttgtt
    17401 gtttaaatgc gggtatcatg ttgctacgga tgatatctcc agccagagca tcggttacct
    17461 tttcttccag tccataacct acctggatgc ggacagtgcg atccgaccag gcaacaatga
    17521 tcagtatccc atcattgcgt ttggcatctc ctaaacgcca attgtcaaaa acccttgcag
    17581 catattgttc aattgtctcg tctttggttg tgggaataat taatacagca atttgtactt
    17641 gggtattatt gaaaatagat tctagcttgc gcgtcagttc tttcttttct tgtgagttta
    17701 agctactcgt aagatccgta acttgttgct gtaattgtgg gacaatagtt ggttgggctg
    17761 caacaaagga acacacgagt gtgaatatta gtatgaaata gcgcattata aatgtcctat
    17821 taaagctgga taaataaatt gacggcggta tcggtaattt gaggttcgtt ataattaaaa
    17881 atgaatacag aaaagataaa tataattttg gtgaatgttt ttgacatgat taaatccctt
    17941 tactttattt ctttctgaca taatacgtca ctccctttta ttattaacga tatgtttcgc
    18001 atttttcttt aaacctgccg gtaacagaat gttaataaga catgattgtt gtggcaggat
    18061 ctgcaataat taacaattat ccacttcaca gagaatgcta tgcagtaccc gattaacgag
    18121 atgttccaga ccctgcaagg tgagggttac tttaccggcg tccccgccat ttttattcgt
    18181 ttacagggat gtccggttgg ctgtgcctgg tgcgacacca aacacacctg ggaaaagctt
    18241 gaggatcggg aagtctccct tttcagcatt ctggcgaaga ccaaagagag tgataagtgg
    18301 ggggcggcga gcagtgaaga tttgctggct gtcattggcc gtcagggata caccgcgcgt
    18361 cacgtggtga ttacgggtgg tgagccttgc attcatgatt tgctgccgct gactgacctg
    18421 ctcgaaaaga acggttttag ctgccagata gaaaccagtg gtactcatga agtacgctgt
    18481 acaccgaata cctgggtcac cgtgtcgcca aagctgaata tgcgcggtgg ctatgaagtg
    18541 ttgtcacagg cactggagcg agccaacgaa atcaaacacc cagtgggacg cgtacgtgat
    18601 atcgaagcac tggatgaatt gcttgcgacg ctgaccgatg ataaaccgcg agtcattgca
    18661 ctgcagccaa ttagccagaa ggaagatgct acgcgtttat gtatcgatac ctgcattgcg
    18721 cgcaactggc gtttgtcgat gcaaacgcat aaatatttaa atattgcctg atttgattat
    18781 gcatcattga tatttaacgt tcaggatgaa aataatcgcc ccgttttaca ggggcgatgc
    18841 aagatagtga cgggattatc ggtagcgttt ttgtagactg gcaggaagat aacgctgtaa
    18901 tggggatggc gtttcacctt caatcagttg cgaaataagt tcaaagcagt gaaaagcaag
    18961 ttggcgatta tcctgttgta ttgtgtcgat tcttaccgaa agtgaatcat acaagtaatg
    19021 atcatcaaaa ctcgccaggt ggattttact gtcgagtaaa ttatattggc tcatatagcg
    19081 cagaacgcct tccagtaatc cacaagcggc agtgaataag gctttagggg gacgtcctaa
    19141 acgagcgcat agcgcggcaa acatttcata gcctgagctc ggatgataat taccgtgaat
    19201 aatccattca ggttgtagcg ttactccggc ttgctgcaag ccttgtgtaa atccttccag
    19261 tcgatcacga gtcggtgaca aacggggttg tccccccaga aaatagaatt catccgggtg
    19321 actccgtgca atatctgcca ccagagcggc ggtgggagaa acagagtcag taatgaccaa
    19381 tggcaatgtg ctgccgttca tgtggcgatc gaacaacaca acaggtaatt gctcgctaag
    19441 tttttgatag tcattatcat gcagcatact ggatgcgacg attaacccat caacctggcg
    19501 ggcaatcata ttgttcacca ccattgtttc ctgtgaagga ttttcatcag tacaggatat
    19561 gaggagttgg acgcccgctt cccgacacaa tgtttccagt tcatgggaaa atactgcaaa
    19621 tccgtagtta gtgatttcag gaacgacgag accaatggta tgactgcggt tgtcccgtaa
    19681 tgagcgggca tgaatgctgg gctgataatg ctgctgttgg gcaatactca gcacacgctc
    19741 acgcgtttcc tgcgcaacac gtaactcttt gccacgacca ttgagaacca gactggcggt
    19801 tgctttcgac actcccgcca gttcagcaat gtctttaata gtaacgcgtt tggtttttct
    19861 cacgacgagg atccgtaatt caaaaccctc attctaccat gcaggcgcgt agcgaccagt
    19921 agcgtaacgt gacgtcagac tcaccctgta atatcagcga agctggatga tggggaaaat
    19981 aacgattact cattacacct tcaccattat tgatgaatat ctctacgctg gaactgtcac
    20041 agagaatctt cagtgacgtt acgcttcctc gccagtaacg agttagtttc tcttgccctg
    20101 caaggcttct cctctgtagt tgaataccat cgtcattaag atgcagtgcc agcgcattgc
    20161 caaaattcag atacagctca cccgacggaa tgatgtccat ttccaggcgt tcaatttcga
    20221 catgcggcgc atgatccgct tttccttgcc agaagtgttc agcttcacgc agttgcgcga
    20281 gttccctgat tggcatctga aacaattttc ccttctggaa tgagagttcg cgaaaacagg
    20341 tcatttgatg ctgccaatga tgctttctgg ttggttgcaa catctcttcg ccatcaggaa
    20401 cccccatcca gccgaccagc aatcgtcgcc cgtccggggc ttccatcgtc tgtggtgcat
    20461 agaattcaaa tcccgcatcc agctctgaga aagcgccatg cgtaaacttg cctgtctcgt
    20521 aatcaaactg tccatgtagc caggcgcagg gataagtatt cagatagcgc tcatgttcgc
    20581 gcgccattcc ctgggggcag cagaggagaa tgtattcacc atcaagggcg aaaagatcag
    20641 gacattccca catgtatcct gcgttatcta tttcattcag accattacca gcaatttctc
    20701 catgaaaaga ccaggtatag agatcgacag aggaatacag cagtactttc ccccgtttct
    20761 ctttatcttg cgcgccaagc accatgtacc attggctgtt gtgcttccag acttttggat
    20821 cacgaacatg tccggtgtaa ccatcaggta acggtatgac agggcctagt ttatcaaacc
    20881 cgccctggtt attttcggtg gccagacatt gccatgctgt tctgctgcca tcatcaaact
    20941 taacattacc ggtatagcaa agtgtgagaa cgccctgatt attaaccgcg cttcctgagt
    21001 aacaaccatt acggtcgtac tctttatcgg gcattagcgc taatggttca tgctgccagt
    21061 gcaataaatc cgtcgaactc cagtgccccc aacatttgta cttatgatca caagccagtg
    21121 gattccattg ataaaacaga tgatagcggc ctccagacca acaaaagcca tttggatcgt
    21181 tcattagtcc ggtgactgga gcgagatgcc aacaaggata gtgggcatct gccagtgctt
    21241 tggactgacc tttcatcact gcctgtaaaa tcgcgggcat ttgtgaaggc atcgacatta
    21301 ctcctcgtcc gttttgtatt tgagtaataa agagaggcta aaagcgacag cgaaagcaat
    21361 caccattcca ataatataat taaccaacga tgttggctga acgattgcca tgccgggtat
    21421 tgcggtcaga ccaacggcag tcatataaac gtgaacagag acaacccagg caccgccggc
    21481 agcgccacca attaaggccg caataaaagg tttaacaaaa cgaaggttga taccaaaaat
    21541 agcggcttca gtaatcccta acattgcgga aaaggccgag ggtagggtga ttgctttaat
    21601 ttttgtatct ttggttttaa accataccgc cagacatgca ccgccttgcg caacgtttgc
    21661 cattgcccaa ataggcagca ggaaattaac accgattgca gggttaccta aaagtcctgc
    21721 ttcaattgcg tggaagctat gatgaatacc cgtaataaca atcgctgaat atagcccgcc
    21781 gaataacaac cctgctaacc agcctgcgtg tgcaatcagc gtgctgagaa cgaaagagat
    21841 accgtcacct aacgctcgtc ctgccggacc aataatcaaa agagcgataa agccagaaat
    21901 gacgacagtc agaaatggcg tcagaatgag atccagagca tcagggataa acctgcgtaa
    21961 ctgtttttcc actatgctca taaaccatac tgccagaagc acggggaaaa ccgttccctg
    22021 atagccaatc atggcgattt caaaaccgaa gaagttcatt gtctgaaatc ccgcagcaac
    22081 gccccaggca tttgtgagtg ccgggtgagt cagaatccct cctaatgtcg cgccaagata
    22141 aggatttcca ccaaattcac gagcagcagt aaagccaata aggatgggga gaataataaa
    22201 tgcggctgaa ctgcacatat ccagcaggat ataaatcgca ttatcggcat tcacccagcc
    22261 atatgttttc accattccca gaagccccat taataaacca gaagcaacaa tggctggaat
    22321 gatggggaca aaaatattag aaagtagccg tgctattcgc tgaaaaggat tgagtttttg
    22381 agcggcgagt cgagcggtat ctgctttact ggattcgcta atacctgcaa cctgaataaa
    22441 ggcagcatag actttattaa ccacgccggt gccaaagatg atttgcattt gccctgaatt
    22501 acgaaaacaa cctttcactc catcaatctg gccaatggca tgttgatcgg caagtgtgtc
    22561 gtcaaccagt accagccgta ggcgagtggc gcaatgtgct gcactggcga tgttctcctt
    22621 accacctaat aaaggaagca acgattgggc gattttatcg aaatccatat aaccctctaa
    22681 ttcattgtta tttcagtaaa agcctcacat ccgtgaggct gtctggtatt aaaaccaggt
    22741 ttccatttgg acaccaaaat tccactctcc gccagtattg aaaccactgc tgccgaaagc
    22801 atcattgctg gcaaaatcat caagtttgct gctccagtcc atccaggtgg caaacaggcg
    22861 aagctccgga cggctaaaga aattattgat atcgttagct tttagagttg gagcaaaggt
    22921 gagtttatag aagctgccgt ttacggcgtt gtggttttga tacccctttg gcttcagatc
    22981 catatattga tagctaccct caaaggccag agcaaaattc tgtgttacct ctttgatcag
    23041 acgtgtattg aaggtcaccc attcgtagct atcgcctttg acgtaacgat ctttactgct
    23101 ttgtgcgaga attgctggcg caacatacca accgcttccc agaggtgttg tgccgtaaga
    23161 tgcgaagcgc caggtattcg cctcagacag cagagcgcca tcggagccaa tccctttgac
    23221 ttccgcaccc aggccatggc catatagcag tgctgttttt gccgtccctt cacgcaggcc
    23281 atagaaagtg tcattgtgca gaccaactaa cgcatgtacg ccagtatttg cagcatcagt
    23341 ttgaatgaga tcaccattgg catcttttcg atcatcatta tcttttgccc gtaatccgct
    23401 aaccatcaac tggaaggggc ctgcataatg attcatggtg aggatgtagt tctgaacgtt
    23461 attgtcgata tcatcaagat cgccgaaatt acgtccgtag agagaaaagt tactgcggaa
    23521 tgtctcgttc cattttacgt catagatacc gccgcccgtt cccgctaaaa atacgacatc
    23581 ggagtctaac cagtgaatat caaaattgtc gcgatcaaag cgtttaccag cccagacagt
    23641 actgtctttg aatgctccgg taaaacttgg taatgcgccc agttcggcaa aagcctgtcg
    23701 gatattcagg ttactggagc cgccagtcca gtcgttgtaa tctctttgtc cgtcagccaa
    23761 cattgcttta aagcgtgtgg tcgcaccgtt atccagtgtt tgtttatgtt ctacatttaa
    23821 ctcgacatag gtatcggctt catttcccag acggccaaca gctccaccag tttcacctgc
    23881 gggagtcaga tacggcccac ttttgctact ggaagcggca tcattcatca gtaacccgga
    23941 acgggcatag ccatgaaact caaaaccact gttttctgcg gatttctgtt cgagctgaac
    24001 tgttctctgt gctacttctt gagttgttag ctgattttgt tgttgtgttt cagccagttt
    24061 ctgaacctgt aattcagctg ttttggccct tgcttctgcc gcctgggcgc gggattccgc
    24121 attttttaaa cgttgttcca acgccgccag tcgagattcg atactgctaa tatccgtttg
    24181 cgcatgtacc gttgtagcac cggtcagcaa agcaattaac actgccaaag ttgttttttt
    24241 atacataatt tgtcgcgtcc catcagagaa ttataaaaat tgctaaaatg ctaaaccggt
    24301 ttaggtgttg ataataaagc tgacttttta gccagagcaa ttattttcgc taaaccgatt
    24361 tagtaaatgt gacaggcacg acaaatcggg ttacagatac aacagcagga tcactattgt
    24421 gaagggagat cgtgttgtcg gggtaaggcg gtcatcgcgc ctttagccgt ggtcgccaga
    24481 gcaccacaaa tctgagcttg tgcaatgatt cgttcaagct gtctggtatt tgttggtatt
    24541 ccagaatcag ccagaccggc aagtaatcca gcgacaaaag catctcctgc cccggtcgta
    24601 tcgacgacag aaacaggttt ggcgggatag tggataaact gcccctgaaa atacacaagt
    24661 acgccatttt ttccttgtgt aataagcaat aaagtcaacg gataacgttg tactaatgaa
    24721 tatgcgcctt gctgaacctg gctttcgtca ctgatgaata acaactcttc ttcggacaat
    24781 ttagcgatat tcgccagcga aagcgcacga tcaaggtatt tccttaacag cgcttcactc
    24841 tgccagagat cgctgcggat attgggatca aaactgatgt ttccgccagc ctgacgtatt
    24901 ttctccatag ccagaaatgc ggtacttcgg gaaggttctg cactgagcgc aatggaacaa
    24961 agatggagcc attcacccgg cccaaatgca ggaaggtcat caggttgtaa aaaaagatcg
    25021 gcacttgggc gtaccataaa ggtaaagttt ctttctcctt gctcatcaag tcctacagcc
    25081 acaattgagg tgcgatgttg aggatcaaga gacatataac gtgtatcaac attttctgta
    25141 ctcagtgttt gatacataaa gcgaccgaaa ggatcatcgc caactttgcc aataaaggca
    25201 cttttccccc ccagccttgc gataccgact gcaacattag caggcgcccc accaggacac
    25261 tgtagtagtc tcccctggct ttcgggtaat aaatcaacaa ccgcatcacc gagtacccat
    25321 actcttgtgc tcataaaggc tccaatatac taattgctaa actaattaaa tcggtttagc
    25381 aattaaagca taaacgctgc tcaggagaga acaatattca gcagtggtta cccggtattt
    25441 tgtagtggaa acgtgaatat gccgggatta atcatctacc cggcactgtt tatctcactt
    25501 acgcattcga ccagctctgg atcgaatccg cattccccaa ttccccatgc atcacttcat
    25561 acgatttttt caggatcaca tcagtatgtt tggttaggtc tttgaaaatg cctttattca
    25621 tcgcgtcgta gcgtttggca ttgctctcaa tcggtatgaa gatatctttc acgcgaacca
    25681 ttttatcgac agccgttgtg taatccgggt atagccccag acctaccgcc gtattgatcg
    25741 cagcccccag acttgcacaa ccgttaatgg cgtttcggcg tgccggaagg ttgaacacgt
    25801 cggcaaaaat ctgcataaac agatcgctgt tcgaaccgcc gccagtaatg atcacatgct
    25861 ttgcaaagtg attcatttca tgacacatat tgtcgtaatt attcttcagc gtcagcgcca
    25921 cgctttccag tatcgaacga tatatccatg cgtaatccat gctggaatca aaaccgatca
    25981 taatcccgcg tttgtacggt tcccacggat tggtcagcca gtccagcacc gtcatcagac
    26041 cattacagcc aggcggcacg caagacgctt ttttgttgag caaatcttcc ggtgaaagat
    26101 cctgtgcttt ggcatcctga attaacgact cgcctaacat gtcgcgcagc cagctcaccg
    26161 tccacatgcc tttgcgaata ccgtaacctt catacagcaa tgtttgcgga atagaagaca
    26221 taatcggcca gtacgccacc ggatctttcg gcagtgcttt gccgttcatc atcaacgcga
    26281 tataggtgcc aagagaaatt accgccgttt catcatccag taacccggct cccagagctt
    26341 ctaccggttt atcactggtg gtacaaacaa ccggcagccc agccgggaaa tgtgtcgcca
    26401 gcgcagcttg tggtgtgata tgcccaagaa tggtgcccgg catttgcacg tcaaaaagca
    26461 tctggcgggg gatattaaac ttttccatta ccgtagcatc ttcgctccat gcccagctct
    26521 tataatccac cggccactga ccaaaatagt tggcgatatt gtctttaaac tcgccggtta
    26581 agcgatgtgt cagataaccc gaaaaagagg tgacataggc cacgtcagga ttagtgtgtt
    26641 cgtaagggcg tgtaacgcgt gcatcctgcc agctaatcaa cggcgcggct ggcgtaccat
    26701 cggctttcaa tagcgcacgg cagcaacgga ttgagcccag gccaatgccg acaatatctt
    26761 ctttattccc ggcaaactgg ctcatcaaat cgtgactggc aaaacatagt gatgcccata
    26821 aatcatcgtc aggatgttct gcggtatcgg catctggcgt gtgcatcggc tgcaataagc
    26881 ctttgccttc gcaaaccaca ttaccttcca gatcgtacat caccactttt gtgctctgac
    26941 ttccgccatc aatccctatg atgtattttt tcgacatgat ttttctcctg taattttcct
    27001 ggcttacttg ctgctagcca ttgcgggggc gctatcagca gtacgaattt tttggaacag
    27061 caaaaaggta aagatgatca ccatgccaag cgccgccatg cccattagcc acatgttgcg
    27121 ataggcttct gcagcgggca gggtgtcttg ccagtagccg ataatcgggt aaacgaagac
    27181 atccggcagg aatccaatca ccgaacaaat acctaccgtg gtgcccataa tgtaagacgg
    27241 tgttctcgct tcgccagggc aggcccagta cagcccgcgt gaggcgtaac aggtgaatcc
    27301 cagtaataaa atcaagccaa tccccatcgc gaccgattgc gggttagagt tcgtgacgag
    27361 cagggctgtc aacgccagca aaccgatgat ggaaaggatt tggaccacgc gggtagggga
    27421 tttcacttta ctgtaggtgg tgataatgcc gccaagtggg ccgcacagcg cgcggaagat
    27481 tttgttgatc acaatcccca tgtagctcgc cgccaccagc gacatgccgt acatttcggt
    27541 cagatagttg gtggagtaac tcagaatggc gtagatggtg aagacaccaa aaatcaccat
    27601 gctgcaatac caggtagtgc tgatgcgcaa aacggccagg atgtcgctaa gctggaatga
    27661 ctgtttttct tcgttattgg cactgcgtag gttgttgtta tcgctaacaa aaaaccaaca
    27721 caaaatgccc agcagaatgt aaaccacact atagataata atgaccgttt tcaggctggc
    27781 gctgtcatcc ggtgcaaagc gagaaaagac ccacatggta aacaccgcca gcgacattac
    27841 accgacgccg cgcagccctt ccatccagcc cataattttc ccttgctcgc tatgatcgcc
    27901 cagcaacgat gcggctttaa tcgacaccga ccacagcatt aaaatcgtcg ttatcgcaaa
    27961 ggcgacctga atacagagca ttacccacag cggtggatat gttgccatta acagacccag
    28021 taatccggta atgatcatcg cggaagtaat cattttgcga tgtgaaaatt tatcggcaat
    28081 aacgccgcta ggggcataga aaataatggc cgcaatacca aaggtactca ttattaaacc
    28141 tatctcggta ttgctgaatc ccataaattt tgccatggga atttgataaa tataacgtaa
    28201 ataagccagg tcgaaactaa cgccgccgct aaaactaatt atcgcgaggg ttatccaacg
    28261 gcgatatgag ttgtgttgca tatcgtattc ccatttgtaa tagacaaaaa aaagagaaaa
    28321 gtaagccgcc ctttcaggtg gattactttt ctcaggtctc tagtaattgc aataagaaat
    28381 aacatggaaa gcagaggtgt tgtgtgagag taatcacgct tctgcttttt taaattattt
    28441 gacaatacga acatcacact gtgattacga tcacattagc agttatgtcc ttatgctaaa
    28501 aattatctcc agttaccaga ga
//
LOCUS       DAOFSV010000050        27350 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.050, whole genome shotgun sequence.
ACCESSION   DAOFSV010000050 DAOFSV010000000
VERSION     DAOFSV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.050"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(51..827)
                     /gene="sseB"
                     /locus_tag="QBH27_002276"
     CDS             complement(51..827)
                     /gene="sseB"
                     /locus_tag="QBH27_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDQ7014579.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(969..2252)
                     /gene="pepB"
                     /locus_tag="QBH27_002277"
     CDS             complement(969..2252)
                     /gene="pepB"
                     /locus_tag="QBH27_002277"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDQ7014580.1"
                     /translation="MTEAMKITLSTQPADVRWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPELDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLICNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2311..2511)
                     /gene="iscX"
                     /locus_tag="QBH27_002278"
     CDS             complement(2311..2511)
                     /gene="iscX"
                     /locus_tag="QBH27_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDQ7014581.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2523..2858)
                     /gene="fdx"
                     /locus_tag="QBH27_002279"
     CDS             complement(2523..2858)
                     /gene="fdx"
                     /locus_tag="QBH27_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDQ7014582.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(2860..4710)
                     /gene="hscA"
                     /locus_tag="QBH27_002280"
     CDS             complement(2860..4710)
                     /gene="hscA"
                     /locus_tag="QBH27_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDQ7014583.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(4727..5242)
                     /gene="hscB"
                     /locus_tag="QBH27_002281"
     CDS             complement(4727..5242)
                     /gene="hscB"
                     /locus_tag="QBH27_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDQ7014584.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5338..5661)
                     /gene="iscA"
                     /locus_tag="QBH27_002282"
     CDS             complement(5338..5661)
                     /gene="iscA"
                     /locus_tag="QBH27_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDQ7014585.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(5678..6064)
                     /gene="iscU"
                     /locus_tag="QBH27_002283"
     CDS             complement(5678..6064)
                     /gene="iscU"
                     /locus_tag="QBH27_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDQ7014586.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6092..7306)
                     /gene="iscS"
                     /locus_tag="QBH27_002284"
     CDS             complement(6092..7306)
                     /gene="iscS"
                     /locus_tag="QBH27_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDQ7014587.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7418..7906)
                     /gene="iscR"
                     /locus_tag="QBH27_002285"
     CDS             complement(7418..7906)
                     /gene="iscR"
                     /locus_tag="QBH27_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDQ7014588.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8177..8917)
                     /gene="trmJ"
                     /locus_tag="QBH27_002286"
     CDS             complement(8177..8917)
                     /gene="trmJ"
                     /locus_tag="QBH27_002286"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDQ7014589.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9036..9839
                     /gene="suhB"
                     /locus_tag="QBH27_002287"
     CDS             9036..9839
                     /gene="suhB"
                     /locus_tag="QBH27_002287"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDQ7014590.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9984..10838
                     /locus_tag="QBH27_002288"
     CDS             9984..10838
                     /locus_tag="QBH27_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7014591.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSLAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11029..12309
                     /gene="csiE"
                     /locus_tag="QBH27_002289"
     CDS             11029..12309
                     /gene="csiE"
                     /locus_tag="QBH27_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDQ7014592.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12301..13440)
                     /locus_tag="QBH27_002290"
     CDS             complement(12301..13440)
                     /locus_tag="QBH27_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDQ7014593.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            13810..14232
                     /locus_tag="QBH27_002291"
     CDS             13810..14232
                     /locus_tag="QBH27_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDQ7014594.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(14280..15152)
                     /locus_tag="QBH27_002292"
     CDS             complement(14280..15152)
                     /locus_tag="QBH27_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDQ7014595.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(15164..16258)
                     /locus_tag="QBH27_002293"
     CDS             complement(15164..16258)
                     /locus_tag="QBH27_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ7014596.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(16291..17289)
                     /locus_tag="QBH27_002294"
     CDS             complement(16291..17289)
                     /locus_tag="QBH27_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7014597.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(17292..18821)
                     /locus_tag="QBH27_002295"
     CDS             complement(17292..18821)
                     /locus_tag="QBH27_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ7014598.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLKKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSTDEFYRG
                     T"
     gene            complement(18844..19827)
                     /locus_tag="QBH27_002296"
     CDS             complement(18844..19827)
                     /locus_tag="QBH27_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDQ7014599.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(19924..23205)
                     /locus_tag="QBH27_002297"
     CDS             complement(19924..23205)
                     /locus_tag="QBH27_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDQ7014600.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETESMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLEDNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            23323..24516
                     /locus_tag="QBH27_002298"
     CDS             23323..24516
                     /locus_tag="QBH27_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDQ7014601.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLNPEKLTTAQLIAAWQSGEPWFTSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(24580..25833)
                     /gene="glyA"
                     /locus_tag="QBH27_002299"
     CDS             complement(24580..25833)
                     /gene="glyA"
                     /locus_tag="QBH27_002299"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDQ7014602.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            26162..>27350
                     /gene="hmpA"
                     /locus_tag="QBH27_002300"
     CDS             26162..>27350
                     /gene="hmpA"
                     /locus_tag="QBH27_002300"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDQ7014603.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAVAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
BASE COUNT         6490 a         7658 c         6776 g         6426 t
ORIGIN      
        1 ccttcagagc agcaagtaag cccgaatgcc gccctttggg gcggcatatt ttagattatc
       61 cggttctgtt taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata
      121 tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatg
      181 ggttcatcac caggtaatgt atcggtcgct acacttcccg ccacctgaat aatttcttcg
      241 atatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta
      301 attgaacaaa taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtaagtgaa
      361 tcaatcattt gcgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc
      421 agtacttcct ggctactcag cggattccct tcttcaccaa tcaacaaact gatttcacgc
      481 ggcataaact ctttaccggt tggcagtttg gcattaagga agagcgtttc gccaagggtc
      541 atctcaaaca atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt
      601 tgaagtgctt ctaacgaggt aaaaaaagga atgacgctgg tgccgtcttc tttttcccag
      661 tgctgtaaat caagcgcgct atcttcaact actgcctcgc cctgcgccgc cgtaccaggc
      721 acccagacgg tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt
      781 gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt
      841 cattcaacgt tcggggagca agtgcctgat gcgccacgct tatcaggcct acaagggagt
      901 cgcaatctat tggattttcg caatttgtag gccggataag gcgtttacgc cgcatccggc
      961 atattagttt acgccgttaa cagattagca atcgtgcgca caccaagtcc cgtagcaccc
     1021 gcagaccact gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag
     1081 ccttgctgat agttctcaac aaagtgcgac aggaagcccg cagccgtact tgcacctgcc
     1141 ggatacgccg cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg
     1201 tggaattccg ccagcggcag acgccagaac ggttcgttct cttgtgaggc actcgccagc
     1261 aaacgaccgg caagcgcatc gtcaaaactg aacaacgcgt gataatcatt acccagcgca
     1321 gttttcgccg ccccggtgag ggtcgccgca tcaatgatca gttccggttt ctgcgcactg
     1381 gcatcaatca ggccatcggc cagcaccaga cgcccttcgg catcagtgtt catcacttca
     1441 acttttttac cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctgatc
     1501 aggttatccg cacaacagag gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca
     1561 aatgccagcg ccccggtaac cgttgccgca ccgcccatat ccgacttcat cgagtccata
     1621 aacgcggtct gtttgatgct gtagccgccg gaatcaaaag tgatgccttt accaaccagg
     1681 cacgcgtaca ctggcgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc
     1741 ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga
     1801 tcttcgcctt tggtgatccg gtaagtcaca cgatcccctg cgacgttgca gatcagatca
     1861 acagcacgct gtgccagttg cgatggtccc agctcttctg ccggtgcgtt gatggtgtca
     1921 cgcacccagt caatgatcat caggcggtta tccagttcct gacgctgggc atcgtccagc
     1981 tctggccact ccactttacg tgtgcctttc ggggctttgt aaccttgcca gaatgcccag
     2041 cagcgatccg catcccagcc ttcaccgctt aactgaacat gcttaatgcc cagaccatca
     2101 accttgcgcg ccgcacgctg gatcaacccc agatcgtctg ccccgttcaa atgcagggta
     2161 atgccatcat tattaatgct gtaagttgct ttttctcccc agcgcacgtc ggcaggttgg
     2221 gtagaaaggg taattttcat cgcttctgtc attttagtta tccttattcg tttaacagca
     2281 aaacgggcta cccccaggca gcccgttcat tcactcggct tcgtccagcc agaccaacaa
     2341 aatcgcttcg aggattttct cgttcgatgc ctgcgggtcg tcgtcgaaat cttccagagc
     2401 gcaaatccac tgatgcatat cggtgaatcg aaccgttttc ggatcaatat cgggatacgc
     2461 atcgtacagt gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc
     2521 tgttaatgct cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg
     2581 tcggtaacgc gcgcctggca gcttaaacgg ctttccggct ccagccccca ggctttgtcc
     2641 agcatgtcgt cttcctgctc tgagctttcc ggcagcgagt caaaaccttc gcgaacgatg
     2701 cagtggcagg tggtgcaagc acaggatttt tcacaggcgt gttcaatctc gataccgtta
     2761 cgcagcgcaa catcgagaat ggtttcaccg ctattcgctt ccagaacagc gccatcaggg
     2821 cagagatcct gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg
     2881 gcctttcagc gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg
     2941 tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc
     3001 actcaggtga gcggcagcat cgtcgataac ctgacgttct gcggcgctta acagcgcggc
     3061 atcagcagcc agcgcaccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc
     3121 tgccagcatt cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga
     3181 ggcgatttcg ctatcggtca ggccataaga cggtttgacc tgaatagacg cctcaacgcc
     3241 ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac
     3301 gcgaatatgc gcaccgccag ccggtagcgc cggaatacct cgcagcgcaa aacgcgccag
     3361 cgagcggcag tcctgcacca gttcgcgctc accctgcatc acatggatag acatcgccgt
     3421 ctgaccatct ttaaaggtgg tgaaatcctg ggcgcgggcc accggaatcg tggtattacg
     3481 cgggatcact ttctccacca ggccgcccat cgtttcgagg cccagcgaca gcgggatcac
     3541 atccagcaac agcatttcgc tgtccggctt gttacccacc agaatatccg cctgaatcgc
     3601 cgcgccaata gcgacgactt tatccgggtc gatagaagtc agcggtggac gaccgaaaaa
     3661 ttcgcctact cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc
     3721 cagcacttca tcagcttcta cgcccgcatc tttcagcgcg cgacgacaag ccagtaaggt
     3781 tcgttttacc agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca
     3841 gcccgcaacg ttaacggtca cagagtccgc atcgctcagc gcgattttgg ccgcaatggc
     3901 agcatcgagc agttcacgct gaacgcggtt atcgctacga tcaggaatgc ccgcctgctc
     3961 gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc
     4021 ggtcgccagc acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc
     4081 gccgccgagg tcataaacag cgatcacgcc ttcctggccg gaatccagcc cgtaggcaat
     4141 agccgcagcg gtcggttcat taagtaagcg cagaacatgc agccccgcca gacgcgccgc
     4201 gtctttggtg ccctgacgct gggcatcatc aaagtacgcc ggaacggtga taacgacacc
     4261 atccagctcg cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc
     4321 agaaacgcgc accgggttca gcagccccgc cgccgtttca atcatcggca ggccattctc
     4381 gctggcctgg aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg
     4441 ccccatcagg cgtttaacag aactaatggt gttggcggtg tcgagcgctg cgttagtacg
     4501 cgcgtcataa cccaccgaat gcccttgctg ttgatagtga acaacagacg gcagcaggtg
     4561 acggccttca tggtcagcta acgtttcggc ctgaccgctg cgcactgttg ccaccagcga
     4621 gttggttgtg cccaggtcaa taccggccgc caggcgacgc tgatgcggcg cagcactcaa
     4681 accaggttca ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca
     4741 gtttttcttc gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca
     4801 cagtatccgc cgccgcgtcc cacgtctcgt tgtctaactg ttcaaccatc aactgatggc
     4861 gggtatcaaa catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg
     4921 cctgttcgat ctcgtccagt tcttcgcgca gttccagctg ttccatcagg aacgcggtgt
     4981 cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg caaagaaagc aaatattccg
     5041 cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt aatggtcgcg gattgctgta
     5101 cggcggcgag ttgttccgcc tggcttccgc tggcgaattt atcaggatga tactgacgtt
     5161 gtagatcctg aaaacgcagg ctcagcgcct gggtatcgag ttgataacgg gcaggcaagc
     5221 caaagagggt gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga
     5281 atcaggtaaa acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca
     5341 aacgtggaag ctttcgccgc aaccacactc atctttgacg ttcgggttgg tgaatttaaa
     5401 cccttcgtta aggccttctt tgacgaagtc cagctgcgta ccgtccagaa attgcaggct
     5461 tttgccatcg accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg
     5521 ttcgtcaaca aattccagta cataagccat acctgaacac ccggaggttc tcacgcccag
     5581 acgcaggcca aaccctttac cgcggttagc cagaaaggta tttactcgcg ctgctgcact
     5641 gtcgctcagt gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt
     5701 tatagtccgc aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca
     5761 ccggcggcag ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga
     5821 gagacttccc tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc
     5881 cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact
     5941 tcatcacgtc gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt
     6001 tgtcaaagga acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag
     6061 ccattataaa ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca
     6121 gatccacgcc ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg
     6181 atttacgaac taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag
     6241 agaaacggat agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg
     6301 acggttcgag gcttgctgac gtacaggcgg aacctgaaga aaccgcgagg tctttcagcg
     6361 ccataatcag cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac
     6421 cgtgttccag gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac
     6481 gattacgcag gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat
     6541 aggcttcgcc cataccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct
     6601 cgtgaccgcc gccatgcatt tgtgcttcga tacgtacgcg cggtttacga cgcacataca
     6661 gcgcaccaat acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt
     6721 tcaactggct cagatcgatc ggcagtttgc ccacgctctg ggttgcatca acgtgataga
     6781 taatgccacg agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt
     6841 cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgct gcttcgagtt
     6901 ctttcaggtc gataataccg ttacgctgcg gtgccaggta agtgacttca aaaccttcgc
     6961 gctccagctg acggcaggta tccagtaccg ctttatgttc ggttttactg gtgatgatgt
     7021 gcttgccttt tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg
     7081 ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat
     7141 tacgggcgat atctaccgct tcttcagcct gccagccgaa acggtgagaa cgggaggccg
     7201 ggttaccaaa ggttccgtcc atcgtcataa actgcatcat tttctcggca acacgcgggt
     7261 ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc
     7321 cgtacatcac tcaatgcaag gaaccaggct accggctgga tgtacgaccg tgtttacgga
     7381 gtgtttagca ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga
     7441 tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca
     7501 gcacttcctg gttattaacc agttcgccta aagtaatgtt attgagaaaa ccggtaagac
     7561 ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc
     7621 ctttaccctg acaacgggtg gcatctacag attcgtcaac ggcgctaatc acctcgccaa
     7681 cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg
     7741 aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc
     7801 cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt
     7861 caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct
     7921 caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc
     7981 aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt
     8041 taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa
     8101 caccttatcc gacctgggat tccgtgcact tacaggcctg ataagacgcg ttaagcatcg
     8161 catcaggcaa ccactgttat tcggccttgt tacctttatt ctgctgctca atagaagcca
     8221 gaatcccgcg caggatattc aattcctggc tttccgggcg cgcacgggta aacagacggc
     8281 gcaatttatt catcacctgt cccggatggt tttcacggat aaaaccggtt gccagcagcg
     8341 tttgctccag atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt
     8401 catgctcgac ctgctcgcca ttttcctgag ttgccagcca ggccatgcgc acttcatagg
     8461 caataacctg aaccgccatc gccaggttca gcgaactgta ttccgggtta gccgcaattg
     8521 cgacatgata atggcatttc tgcaactctt cattggtcaa gccgacgcgc tcgcgaccaa
     8581 acaccagcgc caccggggta tttgccgctt cagcgacgct tttcagaccg cattcgcgcg
     8641 ggtcgagcat cggccacggc agcgtgcggg agcgagcgct ggtgcccacc accagactac
     8701 aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg
     8761 cccctgctgc cagggcaatc gcctgggagt caggtttcac tagtggatta accagccaca
     8821 gattggttaa tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg
     8881 acgtctccac cagcacaatt cgaatatttt gcagcattgt ctttcttcat ctaaagatta
     8941 ttcacgcatc ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg
     9001 ttttcccgtt ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg
     9061 tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg
     9121 tagaagcgag ccagaaaggc agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag
     9181 cggtgattat cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa
     9241 gcggtgaact tgaaggtact gatcaggatg ttcaatgggt tatcgatcca ctggatggca
     9301 ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag
     9361 gccgcaccga agttgctgtg gtatacgatc ctatgcgtaa cgaactgttc accgccactc
     9421 gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg cagcaccgct cgcgatctcg
     9481 acggtactat tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc actacctaca
     9541 tcaacatcgt cggcaaactg tttaacgaat gtgcagactt ccgtcgtacc ggttctgcgg
     9601 cgctggatct ggcttacgtc gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc
     9661 gcccgtggga tttcgcggca ggcgagctgc tggttcgtga agcaggcggc atcgtcagcg
     9721 acttcaccgg tggtcataac tatatgctga ccggtaacat cgttgctggt aacccgcgcg
     9781 ttgttaaagc catgctggcg aacatgcgtg acgagttaag cgacgctctg aagcgttaat
     9841 gactcaggcg ggtgatatca ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact
     9901 tcctcaccgc tttactttct ttcgtcactc tcccaccatc tttcgccgat taatggatga
     9961 taaagaagtc gcaaggaaag gttatggcgc tgccagtgaa caaacgcgtt ctcaaaattc
    10021 tgtttattct ctttgttgtt gccttctgcg tttatttagt gccgcgcgtc gccatcaact
    10081 tcttctatta tcctgacgat aaaatatacg gtcccgaccc ctggtcggcg gaatccgtcg
    10141 aatttacggc taaggacggt actcgtctgc aaggctggtt tattccttct tcgacgggcc
    10201 ctgctgacaa cgccatcgca accatcattc atgctcacgg caatgccgga aatatgtccg
    10261 cccactggcc gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt
    10321 atcgcgggtt tggtaaatca aaaggcacgc cgtccctggc cggattgctg gacgatacgc
    10381 aaagtgccat caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt
    10441 tcgggcagag tattggcggg gcgaatattc tggatgttat tggtcagggt gatcgtgaag
    10501 gcatacgtgc ggtgatcctc gactccacct ttgcctctta tgcgaccatt gccaaccaga
    10561 tgatccccgg cagtggctac ttacttgatg agagttacag cggcgaaaat tacatcgcca
    10621 gcgtcagccc gatcccgctt ttactcattc acggtaaagc tgatcacgtt atcccatggc
    10681 agcacagcga aaagttgtat agcctggcaa aagagccaaa acggttgatc ctaatcccgg
    10741 atggcgaaca cattgatgct ttttccgatc gtcacggcga tgtttatcgc gaacagatgg
    10801 tggattttat ccttagcgca ttgaatccgc agaactaacc catgatcgct agcacgataa
    10861 tcattcacaa aaccacctta agacatgcta atccactggt cagaacagtt taagatgaga
    10921 aaaattctgt gatgcttgcc aacatttctg atgattagca ttcccttcgc catttccctg
    10981 agcaaacttt agctattctt atcaattatg cttatgggag atctacagat gatgcctacg
    11041 cttgctccac catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacgctc
    11101 tttcagccgg ggttgaccgc caccacggca actttcagcg agcttaatgg tgtggatgat
    11161 gatatcgcca gtcttgatat cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc
    11221 acactgacga ctggttatga cggcagctac cgggttgaag gtacagtgct taaccaacgt
    11281 ttgtgtttat ttcactggct acgacgtggt ttccgtctgt gtccgtcatt tattaccagc
    11341 cgattcaccc ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttac
    11401 gacgatacca atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt
    11461 gaaacgcgcg atattcattt cctgtgtctg tatttgcaat attgtttgtt gcaacatcac
    11521 gctggaatta cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa
    11581 tttcaggtag cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtaccg
    11641 cctgatgagc cactatttat ggcactactt ttttccatgt tgcgggttcc cgatccactg
    11701 cgggatgcgc atcagcggga cagacaactg cgtcagtcta tcaaacgtct ggtaaaccat
    11761 tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg ggttatgcga tcagctttat
    11821 acccacctcg cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taacaccctg
    11881 ccggaagagt tcgcccgcct gtatccacgc ctggtgcgca ccacccgcgc ggcgctggcc
    11941 ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg aaagcggtct ggtcgcggtg
    12001 atttttggtg cctggctaat gcaggaaaac gacctgcatg agaaacagat tattctactg
    12061 accgggaatg atagcgagcg agaagcgcag attgagcagc agctacgcga actaacgtta
    12121 ctgccgctca acattaaaca tatgtcggta aaggcatttt tgcagacagg cgctccgcgc
    12181 ggcgcggcac tgattattgc gccttatacc atgccgttac cgctcttttc accaccgctg
    12241 atctatacgg acctgacgtt gacaacacat caacaggagc agatccgcaa aatgctcgaa
    12301 tcagcatgag ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat
    12361 cacccagaac acgccgtggc cgagatattg atacaggaaa ccggcgaaaa cggtcatgat
    12421 ggcgatactg ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct
    12481 accctggcga gcagcaatat agcgcatggc agccaggtgg cagaccgtga aggtaccgca
    12541 atgcagaatt tgcaccacta tcaaccacgg caacgccgtc gttgctccca taatgcccca
    12601 gcgcactacg ccgcaaatcg ccgagatcaa cagcatatcg cgtgcactac aacggcggaa
    12661 aagtttatta ctcagcgcaa aaataatgac ttccgccacc acgcccagcg accacaaata
    12721 ccccaccgcc gaggccgagt agccagctgc ctgccagtaa atggcgctaa aaccgtaata
    12781 ggccgcatgt gccccctgca ataaacaaac gcaggccaga aagcgccagt tctggcgaac
    12841 caacgccaac cacgcagacc agccggtgct ctcctgctgg cggcttgccc cttgtggctg
    12901 aatcgtcgga cggatgagaa agccgagcag catggatgcc acgcccaacg tcaacagcgc
    12961 gaggatcacc cgataatcaa acatactgac cagtttgccc gtcagcgccg agccaatgac
    13021 aaacgccacc gagccccaca gtctcacttt gccgtaatca agcgggaact gcttttgcca
    13081 cgtattcgcc agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat
    13141 catcaccagc attagccacg ctacgtgcgc ccccgcccag aaggcgacag caaagagaag
    13201 tgtcagcagt gccagcacgc gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg
    13261 cgcgatgagc aaactcccga ggaaacgggc aaccagaccc gcccccaata acaggccgat
    13321 ggtttctggc gttaaaccaa tccctttaag ccagacgctc cagaaaggta gaaaaatgcc
    13381 gtaactaaaa aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat
    13441 gagtccctcc cgtcaggagg cgttagtctg gggttaatcg aatgttggtg caagttgaaa
    13501 aataagaata ttcgagcctg cttcctgacg gttatcctgc atatcaggaa atgacaaaaa
    13561 agtgcggccc acaacacaac atgtaaaaaa gaacgatgaa acaggtgaaa atcgcaattt
    13621 attgaactga caaactcatg tgagacggat aaggcgtgca tgtcgcattc ggcaggaaac
    13681 aataagcaca ttgtcagcca tctgcgttat gccgttatac cagaaacgaa tatctcaacg
    13741 cctggggcgt ggcgaggtac cagagtgtgt attacgttta aatcacatca tcttgcaaag
    13801 ggattggtta tgaactcact acgttatttt gattttggca ctgcccgccc cgttttatta
    13861 ttaattgccc gtatcgccgt ggtcttaatt ttcattattt ttggttttcc caaaatgatg
    13921 ggctttgacg gtacggtcca atatatggcc tcgctgggcg cgccaatgcc gatgctggca
    13981 gcgattattg cggtagttat ggaagtgccc gccgcgatat taatcgtgct tggctttttc
    14041 acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacggcggt gattggtcac
    14101 cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt ctggaagaat
    14161 gtcagtatcg ctggcgcatt cctgctgttg gcaattaccg ggccgggggc gatttctctc
    14221 gatcgccgtt aggaaagatg ccggatgtgg cgtgaacgcc ttatccggca ttttataaat
    14281 tacagcaacg caacccgcaa cgacatctct gaaattgttg attcccctgg tgcaagcgca
    14341 atgaggtcac caccttccgg gcgatgatgg tcatccggcg catgactcat cggttccaga
    14401 cagaagaaat caaacgcata tcctttatca aacgcagggt ctgaaacaaa gatgaaataa
    14461 cacggtgcag gtggcgtcgt ttccatgatg atggcatacc cctcctgcgg ctgctcgatg
    14521 cgggccttac cattccatcc ggcaaaacca ttgtttaccc actggcgcgg caacggcgcg
    14581 agctggttaa aatccagctc ctgcggtagc tgctcgcaaa actcacccgc cagccactgc
    14641 tcccgctcca gccagtaacc gctcgcctgt gcctgaatcc gcgtttgcgg cgacaacgga
    14701 aaataaggat gccagccggt accaaatggc agcgtctctg ccccttgatt ggtgacagcg
    14761 agcgtcaccg tcagcgtatc cgccgttaaa tgaaatgcct ggcttacccg atagtgataa
    14821 acaccgctga gatgttcata caccagacac aagctatcat cgctgcgcga gacacattgc
    14881 cactcgccca accagccatc gccgtgtaga tagtgcgcat cccactcaac attcggttgc
    14941 agttgatact cacgcccctg ccagacaaaa cggttgccgc tcacccggtt tgcaaatggt
    15001 accagcggaa aacaagaggc atcggttgcc acaccgcttt ttttaccagg acgtagcagc
    15061 ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct gatcagaaac gtccagcttt
    15121 agcggcccgt gagataaggt atagatggtc atccgcagac tccttaatcc gggaaattaa
    15181 tcacaacttt cccacatttg ccgcttgcca tcagtgcata agcatctcct gcctgttcca
    15241 gcgagaaacg atgggtaatg gcgttacgcg gccacagttt ccagtccgtt aaatcgtggg
    15301 cgcatttttc catatggaac aaactggtca cccaggagcc aataatccgc cgttgatggt
    15361 gcatcaggtc agcgctaacc tcgaactcga cttttccggt ttcaccaatg taaaccacgc
    15421 gtccccagtc agctgtggat tgcagtgcca gtaagcgacc tgcggcatta ccggaacaat
    15481 caagcgcaac atccgcgccg ccgtgagtga gttcggcgat tatctgcggc agaccttcgg
    15541 tggtcgctaa atagccgtga tccatcactc ctaactgttt cgccatcgcc agacgttccg
    15601 gcagcatatc aacgccgatg atcctttttg caccacgacc tttcgccagc atcatcgcca
    15661 tcatgccgac cggccccaga ccgaccacca gcacgttatc gctaccggaa acttcgccgc
    15721 gcaaaatccc ttcatacgct gtaccaacgc cgcaactgat aaacgcacca tcttcatagc
    15781 tcagcgcgtc cggcagcagg atcagatctt tttcttccgc cagcaggtat tcagcatggc
    15841 cgccgtcacg ctgccagccg taagccgctt ttccttcgcc agtacaagaa ataggaaaac
    15901 cgcgacggca gttcgggcaa aaaccacagc cagaaatgtg atacaccagc acgcggtcgc
    15961 cctctttaaa atggcggcag ccttgcccca tcgccacaat ctgcccgcac ggttcatgac
    16021 cgttgataaa tccctggtat aacggtttat cgggtgccgc cgccgtagcg cggtgctggt
    16081 ggtagatata gtggacatcg cttccgcaaa tcccggagga tttcattttg atcagtacct
    16141 ggttaatccc cggcgtcggc accgcaactt cccgcagatc gacggtcgaa tttcctggta
    16201 aataagctgc cagcatcgtt ttcataaatc cctcattaac aatacgatta attttcatcc
    16261 ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc gctttactac tacgctgggt
    16321 cagcaagata tttgccagca ccgccaccac aatgatgacg ccgcgcacca cctgctggaa
    16381 aaaggagtta atcccgagca gcaccagacc gttaccgatt agcgtaatca ccagtacgcc
    16441 aagcagcgta ccgaacaacg agccgcgacc gccggaaagc gccgtaccgc cgaccacgac
    16501 cgcggcgatg acgtcaaact ccagaccgtt tgcggcacct gcgttaccgg aaccgaggcg
    16561 cgccgccaac aaaatgccgg tcaccgccgc taataatccc gaaagggtaa aaataagaat
    16621 gcgtacccga cgaacgttaa taccgcacaa ctgcgctgca gtggcattac cgccaacagc
    16681 aaaaaccgag cgcccgaagg cggttttgcg gctaatgaac acaaacagcg caaataacac
    16741 catcatgatc agcgcggata ccggcacacc gagaaattgt ccgcccagcc agtccagcac
    16801 ttcgttttcg ttaattggca ctggcaaggc gttcgtcata aacagcccca ttccgcgcag
    16861 ggcgctccat aaacccagcg tggcaacaaa acttggcacg ttaaacacgc cgcgcagcac
    16921 cccggcgagc gttcccatca gcgcacctaa cagcaacacc agcagacaag caatcgccag
    16981 cggaacttca aattgcagca aaaacgccag gcacactgaa acaaaagcca ccatcggccc
    17041 aacgctgaca tcaatttcac cggagataat aatcagcgtc atcgcccagg cggcgatccc
    17101 gatggtggcg gcatcgcgca gcacgttcat ttggttattc aatgagataa agccaggcgc
    17161 attcagggag aagaccagat aaagaatggc aatcaccacc agcaaaccga tctcattaat
    17221 atggcgactg acaaattgtt tgagcgagac gctcttgccc tgcggcaatg gtaatgacga
    17281 agcagacatc ttcaggttcc tcggtaaaat tcatcagtgg acagaatggc ggacatcagc
    17341 tcatccacat tgaccggcga gtgaaactcc tgcgagaacg tgccgtgctg taataacagg
    17401 atgcggtcac acaccagcgg caactcctcc acttcactgg agataaacac cacgcttttt
    17461 ccttcggcag ccagctcgcg gacaatacgg taaatctgct gtttggcttc gatatcgacg
    17521 ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg catagaccca acgaccgatc
    17581 accacttttt gctgattgcc gccagaaagc gtgccgatgg gcgtttcgct actggcggcc
    17641 ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc ggatggtgga ccattgcagc
    17701 acaccgttgg cgctgatttt ttgccgattg gtcagcactg tattttcgtc aacgcccaac
    17761 cagggaatga tccccgcttc tttgcggttt tctggcgtat agccaatgcc gcgtttcagc
    17821 atgtcgccgt aatcggggcg catgattttc tcgccgttga taacaatttc gccctgttca
    17881 tacgtctcca gaccaacaat cgccttcagc aattcactgc gccctgcccc cagcagacca
    17941 gcaatgccga gcacttcgcc acgacgtagc gtaaaactga tatcctccag cttgggctta
    18001 tggcgtaacg cacggacttc cagcacggct tgatccataa tttcctgagg ggcaaccggc
    18061 gcaatatcaa cgtgatcgcg cccgagcatt agcgacacaa tatgatgcgt agaggtgttt
    18121 tcgagcatca catcgcccgc cacctgacca tcgcgcataa cggtggcaca ggaggcaatg
    18181 cggcgaattt cttccatccg gtggctgaca taaatcaccg ccacgcccag tgccgacatc
    18241 tttttcaccg cgctgatcac cagttcaact tccgcactgg caagcgagct ggtgggttca
    18301 tcaagaatga ccacgcgcgg ctcgcccttc atcacccgcg caatttccac cagttgcttt
    18361 tgcgccgggc ttagcgttga aacaagttgt tcagggctaa cgtcaacgcc cagcgcctgt
    18421 aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa tcatgccgtt gcggcggggc
    18481 cactgaccga ggcagaggtt ttccgccact gtcagccctt ccaccagact taactcctga
    18541 taaaccgcgc gtacccccag ttcagcggca cggcgagtca gcgtagcttc gtcaccttcc
    18601 agtcgcgtct cgccaatcca gatatcaccg ctatccggac gttcgctacc ggtaagcatt
    18661 cgaatgaggg tcgattttcc cgcaccgttt ttgcctaaca gcgcacgaac ttcgcctttt
    18721 ttgagcgtga agttaacgtt atccagcgca acgactccgg gataacgctt atttcctgcc
    18781 accacttttg ctaccgggac tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag
    18841 cgattagggc agaccatcaa cgtgcgttgc cagccactgt ttaccgtctt ccgttttggt
    18901 atagagatcg attggcacct gaatcacttt ttcaccgtcg gcttgtttat tgataacctt
    18961 caatgtttgc gtaaaaacag cattgcccat tttcttacca gaaatatcca ccaccgcttt
    19021 cagcacctgg ttgttttcca gctcctgagc aatttcagtg gtcatatcag aaccgaaaac
    19081 agcaattttt ccggcctgat tttgattacg taccgctttt accgcgccga gtgtcgctcc
    19141 gcccgattcc cccataatgg cgttgagatc cggcgtggag ataatcagtt tttcaccgac
    19201 agaaatcgct ttatctaaaa cagtcccttc ctgattagcg acaatttgcg cgccgggaac
    19261 gcgggttttt aatacttctt caaacccttt acgacgttgc acacaaactt caaaggcttc
    19321 gcaattgatg acggcaattt tcggctggtc aattttgttg gcaataaaat aatcggcggc
    19381 agcgttaccc agttttttac caaattccag cgggtcacca accagatatg ccgagacata
    19441 tttatcgaca cccttttgat taatacaggt gttgtagcaa atcaccggaa tacccgcttc
    19501 actggcgcga cgaacggtac ggctactgcc attttcagac actgccgata aaataatggc
    19561 atcgacatta cgcgccacga gggtatcaac aaaggtactt tctttcgaaa tatcgccctg
    19621 agcgttagtt tcaattaact gtacctgtac tgaagaatct tttgccgcat cctgaacgcc
    19681 ctgacgcact ccggcgtaat atccctgggt atcaaggtat attgcgccaa tagtcatttc
    19741 tttatcagaa gccctggcaa atagtgcgct acccaatagt gtagccatta ataataaatt
    19801 acgggttctt gtcatttttt taggcatagt gcctcctgta gggtttttat ttacaacggc
    19861 ttattttaat tatttgagcg tgagcggcag cacgtgccgc tcaaatatta cagttaatag
    19921 ggtttagtga acgtagctaa aaataaaagg catcacggtg gtgaataatg ccgctttcgg
    19981 ccaggctgga ttaatcgcca tcaattcagc gcgagcagat tcatagtgcg caacctcccc
    20041 taatcccgcg gccgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa
    20101 atcgccatac agcgacaaca agtcaggctg cgagacggca aagaaatccg cctcgatact
    20161 ggttttcgcc atctcttgtg cccactgttt catttcacta aacagttgct gtgcagtttg
    20221 ttgttcgccc agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc
    20281 gttgtaataa ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg
    20341 cgtcgcttca gtttcatcgc cctgcgcgtt ggcgcatact gcctgccaga accagatgtc
    20401 gttgtcagtt tgccccggta aacggccttc gcttaaattc tccggataat gcagcgcggc
    20461 atgaagcagt tcgctggcct gctgcggctc tctgtcatca agatgctgcc aggcgcgtaa
    20521 taactggttg aggataaact ggctggtgac cttcccttcc ccaccttccc acgggtggaa
    20581 tttgcgcgtg gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat gccacaaatt
    20641 gagcagttct gcggtcatgt cgtcgcgttt cagcgcaatt tccagattat cttccagacg
    20701 cgccagtcgt ttctccggtg tgacaccgct taacttatcc agcaaatccc gttcgaaaag
    20761 cagacgtgca tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc
    20821 ataatcgtgc tgcttattcc acgcatggat cgccaacccg cgccagccgt cggcaaactc
    20881 cggcgacatc tctacgcaac gctgccataa ggcaatggct ttgccgtagc tacgcttgtt
    20941 gtagtagaag caggccagta aatgccgggc aaaccagcac tcttcgatac tctccagcgc
    21001 cgccacttct tccagcgtat tcgggaaacg gacaaactgc gggaaggcat caatagcttt
    21061 tgcgaccagt tctccgcgtt cagcttttgg tagcaaactg gcttgcaggt aaagcggtag
    21121 cgtgcgctga cagtccagtg cgttcagcat atctgccgcc agggcgggca tgccccagtt
    21181 aatcagttgc ccggcggtca tcagggcgtt aacgtcgcgt ccctgacaaa gacctcgcca
    21241 ctgcgcgagg gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt
    21301 cagcggataa tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact
    21361 taacaccagt agcaggttat gcaggcaaag cacttcctga ttggttgggc aggcgcgaag
    21421 actttgttgg caaaaatcca gaccagcgtc gaagttacca ttacgcgccg ccagtcgtgc
    21481 cagaccataa tagccgcccg ctttactgtt gccgctccag accgcacgcc agaaatcctc
    21541 ttcggcttgt tgatattgcc cctgacgttc gtaagcactg gcgcgaatca aactcgcctg
    21601 tccgcactgc ggatttttgt tcagcgcatg tgcgcgtttc agtgcctgac tggcatatac
    21661 caccgcttgc gggaaatctg cacggttata ttccagcatt gccagcgcca gattacagcg
    21721 ataatccagc ggatccagcg ccacgccgcg caggtagtaa tcgaacggtg aacggctggc
    21781 gtgatgatat tgctccagat gttgaccgat aaaccaggct tcatctgtac tggtaatgtc
    21841 ttgtgctgcc agtggcgctt tggcgacgtc cggcaacggc aacgcttgcg gctgatgttc
    21901 atgataactc agtacaatat tgccgtcggc atcggagagc tcaatggtca gcctttcagg
    21961 attgataccg tgcaacacgc cctggatggc ggtcgctggt gtcagggcca cggcatcatc
    22021 gagcaacgcg ttgcatttgc cgatttcgcg gatcgccagg cggtatccgt tcaacggaga
    22081 gatggcatac agcccccact caatcccccg ctcactacgc tggagtttta tcaccgcatc
    22141 gcgggaggca ttttgcacca tgcccagaga atgataaggc aggaaatact gctcgaaacg
    22201 cttctcctcg taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa aaataccggt
    22261 catcagttcg atatacgggc cgttattgtc agtcaggctc ttatcccacg cctggccaaa
    22321 ttcactgtgt ccccagctcc actgtttttt acctggcgca atatggtggt tggcaacgtg
    22381 tagcaaacca ccatcttcat cgtgacacca cgcgccaaca aaatcgtact gtgatttttc
    22441 agccatatat gaggttggaa cgggcacatt tttatagcga gaaatgtcca ctccggcgga
    22501 gtagtccact ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttgcc
    22561 gtgatcaaac accgccgtta catccggcgg gaagacgctc tgatgccctt cccccccttt
    22621 cactgccggg ttggcccacc acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg
    22681 actggcgatt tccagcgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa
    22741 accgtgcatc gactccgttt cgccgaccca caccgtctgt gcgccgtcgt catgggcttc
    22801 gagggtgaaa tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc agttaaactc
    22861 aatcccgccg gagatccacg gccccagcag tcccaccagc gccggtttaa tgacttcatt
    22921 gtgataaaca aaatcgcgct gtttcacttt atcccacgcg cgatgcaccc gaccgcccag
    22981 ttccggcagg atcatcactt tgatgtagtc gttttccagc cacaccgcct gccaggattt
    23041 cagggttttc tgctcgctca gcgtatcggt cacgccgtag ggataaaccg cgccggacga
    23101 tccctgataa acgcgatttt ccaggaacat gggatgtata tcctgcggcc cggtttcata
    23161 ggtcgggatc tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt
    23221 aacaagacat actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt
    23281 cgcggaataa tcacgcaatt aactaaacaa ggtttagtga agatgagagc ctgcattaac
    23341 aatcaacaga ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag
    23401 cgcgccaata aatcaacgct ggcccggctg gcgcaaattt cgattccggc ggtcagtaat
    23461 attttgcagg aactggaaag cgaaaaacgg gtggtgaata tcgacgatga aagtcaaacg
    23521 cgcggacata gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgtctg
    23581 aacgtgacgc ccaccagcat tgagtgtcag gtcgctaatg cctgtttaag tccgaaaggc
    23641 gaatttgagt atttacagat tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa
    23701 aaatgctggc atcgccaccg taaattgtgg ccggatcgca ccatcaatct ggctctggca
    23761 atccacggtc aggtggatcc ggtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg
    23821 gcaacaccga ttgaggtgaa gtacctgctg gaagagaagc tgggtattcg ggtaatggtc
    23881 gataatgact gcgtgatgct ggcgctggcg gagaaatggc aaaataattc gcaagtgcgt
    23941 gatttctgcg taatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa
    24001 atttatcgcg gcagtttgta tggcagtgga cagattggcc acaccatcgt taatccggat
    24061 ggcgtcgtct gcgactgcgg gcgttatggc tgcctggaaa ccgtcgcctc gttaagcgca
    24121 ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg ttaatactca gcttaatcct
    24181 gaaaaattga ctaccgcgca gttaatcgct gcctggcaga gtggagaacc gtggttcacc
    24241 agttgggttg accacagtgc caacgccatt ggtttgagtc tgtataactt cctcaacatc
    24301 ctcaatatta atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt
    24361 aataccatta ttcgccagac gggatttaac ccgttcgacc gcgacgaagg accgagcgtg
    24421 aaagcgacac agattggctt tgggcaatta agccgcgcac aacaggtact gggaattggc
    24481 tatttgtatg ttgaggcgca gttacggcag atttgatggc gcgataacgt agaaaggctt
    24541 cccgaaggaa gccttttttc aacagattaa ccgtttcgct tatgcgtaaa ccgggtaacg
    24601 tgcgcagatg tcgagaactt tacctttgat gcgctcgata acggcttcat cattgatgct
    24661 gtccagcacg tcacacatcc agccagccag ttctttcgct tcggcttctt taaagccacg
    24721 acgggtaatc gccggagtac ctacacgaat accggaagtc acaaatgggc tcttcggatc
    24781 gttcggtacg ctgtttttgt tgacggtgat gttagcacgg cccagagcgg cgtctgcttc
    24841 tttaccggtc aggtttttat caaccagatc aaccaggaac aggtggttat cagtaccgcc
    24901 ggaaaccact ttgtagccgc gttcgaggaa cacttctacc atcgctttgg cgtttttcgc
    24961 gacctgctgc tggtaagttt tgaactcagg ttccatcgct tctttcagag caaccgcttt
    25021 accggcgatt acgtgcatca acggaccgcc ctgaccacca gggaaaacgg cagagttcag
    25081 ttttttgtac agctcttcgc taccaccttt cgccaggatc aggccgccgc gcggacccgc
    25141 cagggttttg tgagtggtgg tagttactac gtgagcatga ggaaccgggt tcgggtagac
    25201 gccagcagca accaggcccg caacgtgcgc catatcaacg aacaggtaag caccgatgct
    25261 gtcagcgatt tcacgcattt tcgcccagtc aaccacgccg gaatatgcag agaagccacc
    25321 gataatcatt ttcggtttgt gttctttagc ttgtttttcc agatcggcgt agtcgatatg
    25381 accagtagca tcgataccgt aaggaacgat gttgtacagt ttaccggaga agttaaccgg
    25441 agaaccgtga gtcaggtgac cgccatgcgc cagattcata cccagaacgg tatcgcctgg
    25501 ttccagcagc gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt
    25561 agcgtagtca gcgccgaaca gttctttcgc acgatcgatc gccagttgtt caacgatatc
    25621 aacatactcg caaccgccgt agtagcgttt gcccggataa ccttcagcat atttgttggt
    25681 cagctgagaa ccctgcgcct gcattacgcg cgggctggtg tagttttcgg aggcgatcag
    25741 ttcgatgtgc tcttcctgac gtactttttc ctgctccata gcctgccaca gttcggcatc
    25801 ataatcggca atgttcattt cacgctttaa catccgcatc tcctgactca gctaacaata
    25861 aaatttttgg cctttatagg cggtcctgtt ggacaacggc gaacagtata accgaatcat
    25921 tgtgcgataa caggtcttga caaaggaatt tacgcaaacg attaccttca ggctacgcaa
    25981 ggctttggag aataaagagc ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca
    26041 aatttttcac ttcatcacat tctttctgaa aaacaccaaa gaaccattta cattgcaggg
    26101 ctatttttta taagatgcat ttgagataca tcaattaaga tgcaaaaaaa aggaagacca
    26161 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
    26221 gggaccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
    26281 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
    26341 tgttgccgcc tacgccagca atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
    26401 aatcgcgcag aagcacacca gtttccagat caaaccggaa cagtacaaca tcgtcggtga
    26461 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
    26521 gggtaaagcc tatggcgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
    26581 cgaaaacgcc agcaaagccg gtggctggga aggtactcgc gatttccgca ttgtggctaa
    26641 aacaccacgc agcgcactta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
    26701 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
    26761 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtattgc
    26821 ggtgaaacgc gaagagggtg ggcaggtatc caactggctg cacaatcacg ccaatgttgg
    26881 cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg cagatgacac
    26941 accagtgacg ttaatctctg ccggtgtcgg tcaaacgcca atgctggcaa tgctcgacac
    27001 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
    27061 tgttcacgcc tttgccgatg aagttaagga actgggtcaa tcactgccac gctttaccgc
    27121 acacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
    27181 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
    27241 tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa cagttagtgg atttgggcgt
    27301 gaaacaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt
//
LOCUS       DAOFSV010000051        26999 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.051, whole genome shotgun sequence.
ACCESSION   DAOFSV010000051 DAOFSV010000000
VERSION     DAOFSV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.051"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(54..3203)
                     /locus_tag="QBH27_002301"
     CDS             complement(54..3203)
                     /locus_tag="QBH27_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDQ7014604.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3226..4419)
                     /gene="acrA"
                     /locus_tag="QBH27_002302"
     CDS             complement(3226..4419)
                     /gene="acrA"
                     /locus_tag="QBH27_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDQ7014605.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4561..5208
                     /gene="acrR"
                     /locus_tag="QBH27_002303"
     CDS             4561..5208
                     /gene="acrR"
                     /locus_tag="QBH27_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDQ7014606.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5336..8698
                     /gene="mscK"
                     /locus_tag="QBH27_002304"
     CDS             5336..8698
                     /gene="mscK"
                     /locus_tag="QBH27_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDQ7014607.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSALSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8737..8901)
                     /gene="rsmS"
                     /locus_tag="QBH27_002305"
     CDS             complement(8737..8901)
                     /gene="rsmS"
                     /locus_tag="QBH27_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDQ7014608.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDETSQSE"
     gene            complement(8915..9442)
                     /gene="priC"
                     /locus_tag="QBH27_002306"
     CDS             complement(8915..9442)
                     /gene="priC"
                     /locus_tag="QBH27_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDQ7014609.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDESGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9512..9889
                     /locus_tag="QBH27_002307"
     CDS             9512..9889
                     /locus_tag="QBH27_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDQ7014610.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10042..10593
                     /gene="apt"
                     /locus_tag="QBH27_002308"
     CDS             10042..10593
                     /gene="apt"
                     /locus_tag="QBH27_002308"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDQ7014611.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10722..12653
                     /gene="dnaX"
                     /locus_tag="QBH27_002309"
     CDS             10722..12653
                     /gene="dnaX"
                     /locus_tag="QBH27_002309"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDQ7014612.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQGVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPDPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTFKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    11986..12050
                     /gene="dnaX"
                     /locus_tag="QBH27_002309"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12706..13035
                     /locus_tag="QBH27_002310"
     CDS             12706..13035
                     /locus_tag="QBH27_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDQ7014613.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13035..13640
                     /gene="recR"
                     /locus_tag="QBH27_002311"
     CDS             13035..13640
                     /gene="recR"
                     /locus_tag="QBH27_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDQ7014614.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13750..15624
                     /gene="htpG"
                     /locus_tag="QBH27_002312"
     CDS             13750..15624
                     /gene="htpG"
                     /locus_tag="QBH27_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDQ7014615.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15805..16449
                     /gene="adk"
                     /locus_tag="QBH27_002313"
     CDS             15805..16449
                     /gene="adk"
                     /locus_tag="QBH27_002313"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDQ7014616.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16581..17543
                     /gene="hemH"
                     /locus_tag="QBH27_002314"
     CDS             16581..17543
                     /gene="hemH"
                     /locus_tag="QBH27_002314"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDQ7014617.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17540..18499)
                     /gene="aes"
                     /locus_tag="QBH27_002315"
     CDS             complement(17540..18499)
                     /gene="aes"
                     /locus_tag="QBH27_002315"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDQ7014618.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGAIAEQRQYY
                     TLERRFWNADAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVARRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSHLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18651..19955
                     /gene="gsk"
                     /locus_tag="QBH27_002316"
     CDS             18651..19955
                     /gene="gsk"
                     /locus_tag="QBH27_002316"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDQ7014619.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20085..21761)
                     /locus_tag="QBH27_002317"
     CDS             complement(20085..21761)
                     /locus_tag="QBH27_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDQ7014620.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(22000..23220)
                     /gene="fsr"
                     /locus_tag="QBH27_002318"
     CDS             complement(22000..23220)
                     /gene="fsr"
                     /locus_tag="QBH27_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDQ7014621.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23438..25090
                     /gene="ushA"
                     /locus_tag="QBH27_002319"
     CDS             23438..25090
                     /gene="ushA"
                     /locus_tag="QBH27_002319"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDQ7014622.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25128..25631)
                     /locus_tag="QBH27_002320"
     CDS             complement(25128..25631)
                     /locus_tag="QBH27_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014623.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(25628..26428)
                     /locus_tag="QBH27_002321"
     CDS             complement(25628..26428)
                     /locus_tag="QBH27_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014624.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(26452..26931)
                     /gene="ybaK"
                     /locus_tag="QBH27_002322"
     CDS             complement(26452..26931)
                     /gene="ybaK"
                     /locus_tag="QBH27_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDQ7014625.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
BASE COUNT         6720 a         7034 c         7017 g         6228 t
ORIGIN      
        1 taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg attacacgtt gtatcaatga
       61 tgatcgacag tatggttgtg ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc
      121 accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac catcccgccc
      181 attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact gataaccagc
      241 ggcataacgc cgaggataaa cgccaacgag gtcatcagga ttgggcgtaa acgcatccgc
      301 accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa gtctttggcg
      361 aattcgacga taagtatcgc gttcttcgcc gacaaaccaa tggttgtgag caggcctacc
      421 tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc accgataacc
      481 cccagcggaa cgaccagcat aacggagaac ggaatcgacc agctctcgta cagcgcagcc
      541 agacacagga acacgacaat caacgaaatc gcgtacagtg aaggtgcctg gttgccggag
      601 agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg cagtttgctc
      661 gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc cgcctggcct
      721 aagatttcca tagatggcag gccgttgtaa cgttccagac gcggcgaacc gtactcccaa
      781 cgagaagagg agaacgccga gaacggcacc atctgaccat cagcagcacg aacataccag
      841 tcgccgatat catccggcag catacggtat ttcgcctctg acatgacgta aactttcttc
      901 acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc gcccagagtg
      961 gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc gatatcaatc
     1021 ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcaacat atcagggtgc
     1081 ttcgctgctt ctgcaagcaa ctggttacgc gcctgagtca gtttttcgtg accaaggcca
     1141 gcctggtcaa tcagctcaaa gtcaaagccg gttgcggtac ccagttccac gattgcgggc
     1201 aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcacgtgt tgcacgcatg
     1261 gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg
     1321 aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac ggcgaacacc
     1381 gactcaacgt tgttcttttc tttggtcaga tagtaattcg ttacctcatt gagcactttc
     1441 tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa tacgccctgg
     1501 tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat gccgaccacg
     1561 atgatcagat acagcaccag gtaacgcccc gtactgcgta gaataccgcc tacgctgtcg
     1621 gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa gcctttttta
     1681 ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag agccggagtc
     1741 aggatcaacg ccaccagtac cgacagcgcc attgctgaaa caatggtaat agagaactga
     1801 cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac cgccgacagt
     1861 accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg ggtggcttct
     1921 tttggtggca aaccttcttc cgccatcaca cgctcaacgt tttctaccac aacgatggcg
     1981 tcatccacca acaggccgat ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag
     2041 aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc
     2101 ggaatcaacg tcgcgcggaa gttctgcaag aacagataca ttaccaggaa cacgaggatg
     2161 atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa cggtgtggtg
     2221 tcatacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt cgccagttca
     2281 gcacggattg ccgcagcggt atccagcgcg ttcgcaccgg tcgccagctt gatccccaga
     2341 ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc acccagctca
     2401 attttcgcca catcacgcag cagcacgcgg gaaccatcct gattcacttt cagcaggatt
     2461 ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc gttaagctgt
     2521 tggcctttca ccggcggcgt accaccgagc tgaccagctg caacctgggc gttctgtgct
     2581 ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagttc attcgggttc
     2641 atccagatac gcatcgcgta ctgtgaaccg aacaactgaa cgtcacccac gcccgacgta
     2701 cggctgatgg catctttcat attcgccgcc acgtagtcgg agatatcctc ctgcgtcatg
     2761 gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga tttctcaacg
     2821 ctcacccctt gctgctgtac ttcttgcggc agcaacggca tcgccagctg cagtttgttc
     2881 tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag ggtgatctgc
     2941 acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat accgttcata
     3001 ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc gccagggtag
     3061 gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac cggcagtttg
     3121 aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc aaaaattggg
     3181 cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag acttggactg
     3241 ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt cttgtgcttt
     3301 tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat cgcctgcttt
     3361 cagaccttct gtcaccagcc acttatcgcc gatagcctgg cttgcaacga tcggacgggt
     3421 ttccactttg tcatccgcgc caaccaccag tacggtggca tcgccacgtg gcgtacgggt
     3481 tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca gacgtgcacg
     3541 cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc gtagggtgat
     3601 agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa
     3661 cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt gtttcagcgt
     3721 gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg tcacatcaac
     3781 gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa
     3841 tgcgccttcc gtcacgtttg acttaccaat gcgaccacta atcggagagg tgactttggt
     3901 gtaagccaga ttgattcgcg cagtttcaac ggcagctttc gccgcagtta ccgcagcatt
     3961 cgcctgttgc gcatcagcca gagcctgatc gtactcttgt ttactgatgt actgagtacc
     4021 gagcaatttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt
     4081 cgccagatca cctttcgcac tgtcgtatgc cgcctgatag gtcgcaggat caatctgata
     4141 gagagagaca cctgcttcga tgtcgctacc ttctttgaaa ttacgcttca ggataatccc
     4201 gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt
     4261 tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca tctgctggcc
     4321 accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg agagcatcag
     4381 aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc gagtgtccga
     4441 tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat attatcgtcg
     4501 tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat tcacgaacat
     4561 atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct
     4621 ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggtgagat tgcaaaagca
     4681 gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga tttgttcagt
     4741 gagatctggg aactgtcaga atccaatatt ggtgaactcg agcttgagta tcaggcaaaa
     4801 ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct tgagtccacg
     4861 gtgacagaag aacggcgtcg attattgatg gagattatat tccacaaatg cgaatttgtc
     4921 ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag ttatgaccgt
     4981 atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg
     5041 cgtcgcgcag caattattat gcgcggctat atttccggcc tgatggaaaa ctggctcttt
     5101 gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat cttactggag
     5161 atgtatctcc tgtgccccac gcttcgtaac cctgccacta acgaataacc ctgaatctga
     5221 ctccaggaat tttcctggac attttcgtcg ttgctattct ggttcactgc gtcgtgatat
     5281 tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat tcatcatgac
     5341 tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta ttgcttttgt
     5401 ttttgtcttg ttatgccaga acacggcgtt tgcacgggcg tcatcgaatg gtgatttacc
     5461 gacaaaagcg gacctgcagg cacaacttga ctcactgaat aaacaaaaag atctttctgc
     5521 tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg ataaaatcga
     5581 tcgcgtaaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat
     5641 gcgccaggcg accgcggcgt taacagcact tagtgatgtc gataacgacg aagaaacgcg
     5701 caaaattctg agcgcgcttt cgttgcgcca gctggaaact cgcgttgccc aggcgctgga
     5761 cgatttgcaa aacgcacaaa atgatctggc gtcttataac agccaactgg tttcgttaca
     5821 gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat
     5881 tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt
     5941 gttaatgcag gtccagcagg ctttgctgaa tgccgagatt gaccagcagc gtaaaagcct
     6001 ggaagggaac actgtcttgc aggatacctt gcaaaagcag cgtgattacg tgacggcgaa
     6061 cagcgcccgt ctggagcacc agttacaact gttgcaagaa gcggtaaaca gcaagcgcct
     6121 gactttaacc gaaaaaacgg cgcaggaagc tgtctccccg gatgaagccg cgcgtattca
     6181 ggctaatccg ctggtgaagc aggaactgga aattaaccag cagttaagtc agcgtctgat
     6241 taccgcgact caaaacggta atcagttgat gcagcaaaac attaaagtca aaaactggct
     6301 ggagcgtgcg ctgcaatcgg aacgtaatat taaggagcag attgccgttc tgaagggcag
     6361 tctgctgttg tcacgtatcc tgtaccagca acaacaaaca ctcccctcgg cagatgaact
     6421 ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag tcaaccagca
     6481 gcgtgacgcg ctgttccaga gcgatgcgtt cgtcagcaaa ctggaagaag gtcacaccaa
     6541 cgaagtcaac agcgaagttc acgatgcatt attgcaagta gttgatatgc gtcgcgaatt
     6601 gctggatcag cttaacaaac agttgggtaa tcagctgatg atggccatta acctgcaaat
     6661 taaccagcag cagttaatga gcgtatcgaa aaatctgaaa tccatcctga cgcagcaaat
     6721 tttttgggtg aacagtaacc gcccgatgga ctgggactgg attaaagcgt tcccgcaaac
     6781 gctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag cctggcccgc
     6841 cgtattcatc gccttcctcg ctggtttgcc gctgctgttg attgccgggc tgatccactg
     6901 gcgtctgggc tggctgaaag cgtatcaaca aaaactggct tccgctgtgg gttccctgcg
     6961 taacgacagc cagctcaaca caccaaaagc gatccttatc gacctgatcc gcgcactgcc
     7021 ggtgtgcctg attattctcg ctgtcggcct gattctgctg actatgcagc tcaatatcag
     7081 cgagttattg tggtcgttca gcaaaaaact ggcgattttc tggctggtgt ttggcctgtg
     7141 ctggaaagtg ctggagaaaa atggtgttgc cgtgcgtcac tttggtatgc cggaacagca
     7201 gaccagccac tggcgtcggc aaattgtccg catcagcctc gcgttgctgc cgatccattt
     7261 ctggtctgtg gtggcagaac tctccccgct gcatctgatg gatgatgtgc tggggcaggc
     7321 gatgattttc ttcaacctgc tgctgattgc cttcctggtg tggccgatgt gccgcgaaag
     7381 ctggcgtgat aaagagtcgc acaccatgcg actggtcacc attaccgtgc tgtcgattat
     7441 cccgattgcg ttgatggtgc tgaccgccac gggttacttc tacaccacgc tgcgcctctc
     7501 cgggcgttgg attgaaaccg tttatttggt gatcatctgg aacctgctgt accagacggt
     7561 attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgccgtgcac tggcgcgtcg
     7621 gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc
     7681 gctggagcag gttaaccagc agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt
     7741 cggtgtcatg ttctgggcaa tttggtctga tttgatcacc gtgttcagct atctcgacag
     7801 cattacgctc tggcattaca acgctactga agctggtgct gcggtggtga aaaacgtcac
     7861 catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa
     7921 cctgcctggt ttgctggaag tgctggtgct ctcgcgactg aatatgcgcc agggcgcgtc
     7981 gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga tgacggtgtt
     8041 cggatcgctg ggcgtctcgt gggataaact ccagtggctg gcggcagcat tatccgtagg
     8101 tcttggtttt ggtttacaag agatcttcgg taacttcgtt tccggtttga tcattctgtt
     8161 cgaacgtccg gtgcgcattg gcgacacggt aaccattggt agcttctcgg gtacggtcag
     8221 caagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag tgattatccc
     8281 gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca cgactacgcg
     8341 tctggtgatc cgtctcggcg tagcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt
     8401 gctgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg aagtcttctt
     8461 tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg
     8521 tgaccgtagc cgtactgtcg atgagctgaa ccgtactatt gatcagttgt gccgtgaaaa
     8581 cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga agggcgatga
     8641 agtgacggaa gtgaaacgcg actacaaagg cgacgatccg acgccagcgg tagggtaagg
     8701 acaataaagg gcgacagtga tgccgccctt tattttttac tcgctttgtg aagtctcatc
     8761 gctctgtaac ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca gcacggtgcg
     8821 ggctgggatc tgattttctt ccagcagcac aatcaaatcg acggccagtt tgacatcgtc
     8881 aggggcattt tccagtgaca tactctctcc attgctagcg ggttaaacgc gctaacctgt
     8941 tttcgatttt ttccagcgca tggcggcaac gtgccaggcg cgcttcatag gcttccactt
     9001 cacgatgcag cgtctgctgt tccacgagat cggtcgctcg cgccagacgg gctctacgtt
     9061 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
     9121 agcgggcaat tttcggtggt gcgctatccc actcgcgtag cgaccaggct gtggcttcac
     9181 gcgctatagc ctccagttgc gccgccagat gttctgccag ccaggcgact tgcggcagct
     9241 gttgctgctc aaccgcatga cgaagcgcag ccaggttatc gcccgactca tcgagacaag
     9301 cctgtagtgt tgtcgcacgg gtctgaaaaa ggtgcctgtc aaaacgggca cttagcgtgg
     9361 caaattgcgc taccggggca caacgctgac gcagcgtggc gagctgtcct tccagttttt
     9421 ccagtagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
     9481 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
     9541 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
     9601 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
     9661 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
     9721 gcgcggcgca aaaccacggg cgattttgct tattttgatt acgtttacga tttcgctgtg
     9781 gttcgtccag atgccgtggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
     9841 ttatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
     9901 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
     9961 cggtcaaagg ttaaacaact gttacttttg atacgttcaa aacgcgccgt gagtaccacc
    10021 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    10081 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    10141 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    10201 aatgcgggca ttaccaaagt ggtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    10261 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    10321 gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcaactgga aatccacgtt
    10381 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    10441 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtagc tgacgctgcg
    10501 ttcattatca acctgttcga tctcggcggt gaacagcgtc tcgaaaaaca gggcattacc
    10561 agctacagcc ttgtcccgtt cccgggccat taattatcgt cagtcttgcg ctgctcacgc
    10621 tacgggcagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    10681 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggtcttag
    10741 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    10801 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    10861 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    10921 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    10981 ttgtcgatct gattgaaatc gacgccgcct cgcgtaccaa agttgaagat acccgcgacc
    11041 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctcatcgacg
    11101 aagtgcatat gctgtcgcgt cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    11161 cagagcacgt taagtttttg ctggcgacga ccgatccgca gaaattgccg gtgacgattt
    11221 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    11281 ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    11341 cacgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    11401 gcggtgacgg ccaggtttca acccaggggg tcagtgcgat gctgggtacg cttgacgacg
    11461 atcaggcgtt atcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc gtaatggcgc
    11521 taattaatga agctgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    11581 gtctgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    11641 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    11701 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgta
    11761 tgggcgttga gatgacgctg ctgcgcgcac tggcgttcca tccgcgtatg cctctgcctg
    11821 atccagaagt gccacgccag tcctttgcgc ccgtcgcgcc aacggcagta atgacgccaa
    11881 cacaggtgcc gccgcaaccg caatcagcgc cgcagcaggc accgactgta ccgctcccgg
    11941 aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca
    12001 aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc
    12061 tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg cccggtgcca tcggcgctgg
    12121 aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc gaccactcca gtgatgcagc
    12181 aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc
    12241 cggaactggc ggcgaagctg gcggcagaag ccattgagcg cgacccgtgg gcggctcagg
    12301 tgagtcaact ttcgctacca aaactggtcg aacaggtggc gttaaatgcc tggaaagagg
    12361 agagcgacaa cgcagtatgt ctgcatttgc gctcctctca gcggcatttg aacaaccgcg
    12421 gcgcacagca aaaactggct gaagcgttga gtacgttcaa aggttcaacg gttgaactga
    12481 ctatcgttga agatgataat cccgcggtgc gtacgccgct ggaatggcgt caggcgatat
    12541 acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc ggataataat attcagaccc
    12601 tgcgtcgatt cttcgatgcg gagctggatg aagaaagtat ccgccccatt tgatcgtaag
    12661 cacagcttac gttcgtcatc cttaacgtga ttgagagaga aacctatgtt tggtaaaggc
    12721 ggtctgggta acctgatgaa gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag
    12781 gaagagatcg cgcagctgga agtcaccggc gaatctggcg caggtctggt aaaagtgacc
    12841 atcaacggtg cacacaactg ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac
    12901 aaagagatgc tggaagacct ggtggctgca gcattcaacg acgcagcacg tcgtattgaa
    12961 gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa tgcagctgcc gcctggcttt
    13021 aagatgccgt tctgatgcaa accagcccgc tgttaacaca gcttatggaa gcactgcgct
    13081 gtctgccggg cgttggcccg aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg
    13141 atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc
    13201 actgcgccga ttgtcgcacc ttcaccgaac aggaagtctg taacatctgt tcgaatccac
    13261 gtcgtcagga aaacggtcaa atctgcgtgg tggagagtcc ggcggacatt tacgccatcg
    13321 agcagacggg gcagttttca ggtcgttatt ttgtgttgat gggacacctg tcaccgctgg
    13381 acggcatcgg tcctgatgat atcgggcttg atcgtctgga acagcgtctg gcagaggaaa
    13441 aaatcactga agtgatcctc gccaccaacc ccacggttga aggtgaagct accgctaact
    13501 acattgccga gctttgtgcg caatatgacg tggaagccag ccgaatcgct catggcgtac
    13561 cggttggcgg cgaactggaa atggtcgatg gcaccacgct gtcacactcc cttgccgggc
    13621 gtcataagat tcgtttttaa gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat
    13681 tctcccttgt ccccatctct ccctcatcct gtttttaacc ttaaaatggc attattgagg
    13741 tagacctaca tgaaaggaca agaaactcgt ggttttcagt cagaagtgaa acagcttctg
    13801 cacctgatga tccattctct ctattccaat aaagaaatct tcctgcgtga gcttatctct
    13861 aacgcctccg atgcggcgga caagctgcgt ttccgcgcgc tttctaaccc ggacctgtac
    13921 gaaggtgatg gtgaactacg cgttcgtgtc tctttcgata aagacaagcg cacgctgacc
    13981 atctccgata acggcgtggg gatgacccgc gacgaagtga ttgaccatct ggggactatc
    14041 gctaaatccg gcaccaaatc attcctcgaa tccctgggtt ctgaccaggc gaaagacagc
    14101 cagctgatcg gtcagtttgg tgttggtttc tactctgcgt ttatcgtggc tgacaaagtg
    14161 accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct
    14221 ggcgaaggtg aatacaccgt tgccgacatc actaaagaag atcgtggtac tgaaatcacc
    14281 ctgcatctgc gtgaaggcga agacgagttc ctcgatgact ggcgtgtgcg ttccatcatc
    14341 agcaaatatt ccgaccatat cgcgctgccg gtagagatcg aaaaacgcga agagaaagac
    14401 ggcgaaacca ttatctcctg ggagaaaatc aacaaagcgc aggcgctgtg gactcgtaac
    14461 aagtcggaaa tcaccgatga agagtacaaa gagttctaca aacacatcgc ccacgacttt
    14521 aacgatccgc tgacctggag ccacaaccgc gttgaaggta agcaggagta caccagcctg
    14581 ctgtacattc cgtctcaggc accgtgggat atgtggaacc gcgatcataa acacggtctg
    14641 aaactgtatg ttcagcgtgt gttcatcatg gacgacgcag aacagttcat gccgaactat
    14701 ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa
    14761 atcctccagg acagcacggt aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg
    14821 caaatgctgg aaaaactggc gaaagacgac gcggaaaaat accagacctt ctggcaacag
    14881 tttggcctgg tactgaaaga aggtccggcg gaagatttcg ctaaccagga agcgatcgcc
    14941 aaactgctgc gttttgcttc tacccatacc gattcttctg cgcagaccgt gtctctggaa
    15001 gactacgttt cccgcatgaa agaagggcag gagaaaatct actatatcac cgcagacagc
    15061 tacgcggcag cgaagagcag cccgcacctg gaactgctgc gtaagaaagg catcgaagtt
    15121 ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact atctgactga gttcgacggt
    15181 aaaccgttcc agtctgtgtc taaagttgac gagtcgctgg aaaaactggc tgacgaagtt
    15241 gatgagagcg cgaaagaagc ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc
    15301 ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc gtctgaccga tacgccagcg
    15361 atcgtttcga ccgacgcgga cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg
    15421 ggccagaaag tgccagaagt gaaatacatc ttcgaactga acccggatca cgtactggtg
    15481 aaacgtgcgg cggatactga agatgaagcg aagttcagcg agtgggtaga actgctgctg
    15541 gatcaggcgc tgctggcaga acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt
    15601 atgaaccagc tgctggtttc ctgatgtcat gccggatgac cttcgtgtca tccggcattt
    15661 ttcttttctt catctgcact ttccgcaaat tatcttgcca ttaaccgttt cagcctcagg
    15721 tgcctttctt gaggcaatcg cctgttggtg gtatcgttta tcgctttttc aaaaaattcg
    15781 acacatttta aggggatttt cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg
    15841 aaagggactc aggctcagtt catcatggag aaatatggta ttccgcaaat ctccactggc
    15901 gatatgctgc gtgctgcggt caaatctggc tccgagctgg gtaaacaagc aaaagacatt
    15961 atggatgctg gcaaactggt caccgacgaa ctggtgatcg cgctggttaa agagcgcatt
    16021 gctcaggaag actgccgcaa cggtttcctg ttggacggct tcccgcgtac cattccgcag
    16081 gcagacgcaa tgaaagaagc gggcatcaat gttgattacg ttctggaatt cgacgtaccg
    16141 gacgaactga ttgttgaccg tatcgtaggc cgccgcgttc acgcgccgtc tggtcgtgtt
    16201 tatcacgtta aattcaatcc gccgaaagta gaaggcaaag acgacgttac cggtgaagag
    16261 ctgactaccc gtaaagacga tcaggaagag accgtacgta aacgtctggt tgaataccat
    16321 cagatgactg caccgctgat cggctactac tccaaagaag cggaagcggg taacaccaaa
    16381 tacgcgaaag ttgacggcac caagccggtg gctgaagttc gcgctgatct ggaaaaaatc
    16441 ctcggctaat caagccgcag cagatctgct ttcgggcagg tctgctcatc ctcctcaggc
    16501 aatacctctt acagcaatta gttcttcttc ttcacttttc cgctacaatt atcaacaagt
    16561 tgaatcgata agaggcggta atgcgtcaga ctaaaaccgg tatcctgctg gcaaacctgg
    16621 gtacgcccga tgcccccaca cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg
    16681 acagacgcgt ggttgatacc tcacggttgt tatggtggcc gttgctgcgc ggcgtgattt
    16741 tgccgctgcg ttcgccgcgt gtggcgaagc tgtatgcctc tgtctggatg gaaggtggct
    16801 cgccgctgat ggtttacagc cgccagcaac agcaggcgct ggcgcaacgt ttaccggaga
    16861 cgcccgtggc gctgggaatg agctacggct cgccatcact ggaaagcgcc gtagatgaac
    16921 ttctggcaga gcatgtagat catattgtgg tgctgccgct ttatccgcaa tactcctgtt
    16981 caacggtcgg tgcggtatgg gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc
    17041 cggggatatc gtttattcgt gattacgccg ataaccacga ttatattaat gcactggcga
    17101 acagcgtacg tgcttctttt gccaaacatg gcgaaccgga tctgctgttg ctctcttatc
    17161 atggcattcc ccagcgttat gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa
    17221 caactcgcga actcgcttcc gcactggaga tggcaccgga aaaagtgatg atgacctttc
    17281 agtcgcgctt tggccgggaa ccctggctga tgccttatac cgacgaaacg ctgaaaatgc
    17341 tcggagaaaa aggcgtgggt catattcagg tgatgtgccc gggctttgct gcggattgtc
    17401 tggagacgct ggaagagatt gccgagcaaa accgtgaagt cttcctcggc gctggcggga
    17461 aaaaatacga atatattccg gcgcttaatg ctacaccgga gcatatcgaa atgatggcta
    17521 atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag
    17581 cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa ggcgtgcagc gtgcctgggt
    17641 agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt ctggtaaagc agatggctgt
    17701 catccagcag cggatcgaac tccgccccgg caataaaaca gggcggaact tcgcgagtga
    17761 gatcattatt aaacaggcag taatatggcg actcgcggtc cgcgtcgttg cttaaatacg
    17821 cctcttcata catctgcaaa tcctgttgcg ttaagccatc ccagacacca cccaacagac
    17881 gacgagccac ggaatcccgt aatccgtaaa gcccatacca cagcaaaacg cccgcaactt
    17941 taccgcaatc gatctgttta tcgcgcaacc acaacgcact ggcgagcgcc agcatggcac
    18001 ctgcggaatc accggcaaaa ccaatgcggg acatattgat ttgataatcc tccgcctgct
    18061 ggtggaaata acaacaggca gccacaattt cctctatcgc ttgcggaaaa cgcgcttcag
    18121 gtgaaagggt gtaatcaatg ccaatcaccg tacattggct gtagcttgcc agcaggcgca
    18181 tgatgcgatc gtgggtatcg agattgccga gaataaaacc gcctccatgc aaataaaata
    18241 gcgtcgccgg gctatctggc tgcggataaa agatacgtgt tttcacctgc ccatattttg
    18301 ttggaaccct gtaagctctg gttgccattt ctggagcgtc cgcattccag aatcggcgct
    18361 caagcgtgta atactgtcgt tgctcagcaa tcgctcccgt tgcaggccag ggcggtaaat
    18421 ccggctgaag agtattcaca acggtcttca tttcagcaga gataaggtcc agaacgggta
    18481 atttgttttc cggcttcatg caaaaactcc tttcaaatta cgtcattgta aggaaccact
    18541 gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg
    18601 tcagattgtg ttaacattcg ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc
    18661 ccggtaaacg taaatccaaa cattacttcc ccgtaaacgc acgcgatccg ctgcttcagc
    18721 aattccagcc agaaaacgaa accagcgctg cctgggtagt gggtatcgat caaacgctgg
    18781 tcgatattga agcgaaagtg gatgatgaat tcattgagcg ttatggatta agcgccgggc
    18841 attcactggt gattgaggat gacgttgccg aagcgcttta tcaggaacta aaacagaaaa
    18901 acctgattac ccatcagttt gcgggtggca ccattggtaa caccatgcac aactactcgg
    18961 tgctcgcgga cgaccgttcg gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca
    19021 gctatgccta tcgttacctg tgtaacacct ccagccgtac cgatcttaac tatctacaag
    19081 gcgtggatgg cccgattggt cgttgcttta cgctgattgg cgagtccggg gaacgtacct
    19141 ttgctatcag cccgggccac atgaaccagc tgcgggctga aagtattccg gaagatgtga
    19201 ttgccggagc ctcggcactg gttctcactt cttatctggt gcgttgcaag ccgggtgaac
    19261 ccatgccgga agcaaccatg aaagccattg agtacgcgaa gaaatataac gtaccggtgg
    19321 tgctgacgct gggcaccaag tttgtcattg ccgagaatcc gcagtggtgg cagcaattcc
    19381 tcaaagacca cgtctctatc cttgcgatga acgaagatga agccgaagcg ttgaccggag
    19441 aaagcgatcc gttgttggca tctgacaagg cgctggactg ggtagatctg gtgctgtgca
    19501 ccgccgggcc aatcggcttg tatatggcgg gctttaccga agacgaagcg aaacgtaaaa
    19561 cccagcatcc gctgctgccg ggggctatag cggaatttaa tcagtatgag tttagccgcg
    19621 ccatgcgcca caaggattgc cagaatccgc tgcgtgtcta ttcgcacatt gcgccgtaca
    19681 tgggcgggcc ggaaaaaatc atgaacacta atggagcagg ggatggcgca ctggcagcgt
    19741 tgctgcatga cattaccgcc aacagctacc accgtagcaa cgtaccaaac tccagcaaac
    19801 ataaattcac ctggttgacg tattcgtcat tagcgcaggt gtgtaaatat gctaaccgtg
    19861 tgagctatca ggtactgaac cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg
    19921 aagacagcct ggaagagtct tactgggatc gttaagttat cgtcggttcg taggccagat
    19981 aaggcgttca cgccgcatct ggcattttgc tctcaatgcc tgatgcgacg ctggcgcgtc
    20041 ttatcaggcc tacatatccg cgcctattat ccggcaacca ccgtttacct cgtcaccacc
    20101 tcacccgccg gtggcgtttc cagcagttcc agcatagtac gggcaatttc acgctcaccc
    20161 atcactacct gattcgcacc acgttcggtg atatacgcca cttcatcgtc ataatgggcg
    20221 cgggcaataa tctcaatatc cggatttttc gcgcgggcag atgccacaat ctcgcccgct
    20281 tcatagccgt tgggaatcgt caggatcagc cattttgcac attccagatg cgccagttgc
    20341 ataatttctt cgttagccgc attgcccaat actgcgcgga ccccgcgctc gcgcagctca
    20401 tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc
    20461 tcccccagca ggctgcctac acgaccgtaa cccaccagta gcgcatggtt gcaaatatcc
    20521 actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg
    20581 gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat aatcgacagg
    20641 atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa tgccattcct
    20701 agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc gatggttaac
    20761 gccgtgcgct gggagtgacc aaacaaacgc acaaggaaca gggccgcgac cgatttacca
    20821 aacagaataa ttgccagcgt cgccagtact gccagcggtt gctgaatcag aattaacgga
    20881 tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagt
    20941 gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac
    21001 gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat ccctaacgcc
    21061 agcgccagca ccgacagggt aaacagctcg cgggaaccgg ttgctgcgct gcgtgccata
    21121 atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc gatcaccttg
    21181 ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc
    21241 attcctgcta ccgcgggtag caataccagt gtcaggacca tcaccaggtc ttccacaatc
    21301 aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc ttcaagtgcg
    21361 cgcagtaaca ccacggtact ggcggtggaa agacataagc cgaacacgat accggtcatt
    21421 aacgaccagc ccagcacggc ggagagcgcc atacccagca gcgtcgccac ggctatctgg
    21481 gcgatcgcgc cgggaatggc gatggacttt accgccatca aatccttgag cgaaaaatgc
    21541 aaaccgacgc cgaacatcag caaaataacg cccagttcag ccagttccgg cgcaagctta
    21601 gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc
    21661 accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg
    21721 ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt
    21781 ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt atgacaattt
    21841 cattgatgat gtttatgaat aattgttgaa ttttgcagaa aaatggaatt agctgcaaaa
    21901 aaaagcacgg atcggaaaac agaaggcggt ttaacagagg aaaggcttat ggcgactgta
    21961 aaacgatgca gccaaagttt gcctttggct gcaatgaaat cagtctttat gccggttatc
    22021 aggcaggaat atggtcaaca tccccaatag tggcaggaaa gcacagattt tatagactaa
    22081 ctctatgctg gtgtggtcgg cgataagccc cagaactgcc gcccccagac ctcccatgcc
    22141 gaaagcaaaa ccgaaaaata gtccagaaac cataccgata cgtcccggaa gcagctcctg
    22201 ggcatagacc agaatggcag agaatgccga agcgaggata aatccaataa tcaccgttaa
    22261 aacccccgtc cagtgcaggc tggcgtaggg taaaatcagc gtaaacggcg caacgccgag
    22321 gatagagccc caaatcacat atttccgtcc aattttatcc cctacaggcc cgccaatcac
    22381 cgtacctgcc gcaacggcaa acaggaaggc aaacaaatga agctgagcat tctggataga
    22441 taatccgaat ttttgcatca gataaaaggt gtaatagctg ctgatgctcg ccatatagaa
    22501 atatttcgag aaaatgagga ttaacagaat gctgaccgcc agaacaactt tattgcgcgg
    22561 cagtggattg ataatcgtcg ctttgggttt tcctttattc attcggtgct gtgccgagta
    22621 ccaacggctg atttgcgcca acaccacgat cgccagcagt gccgcaagca caaaccaggc
    22681 aacgttgcct ttgccataag gcgcgataat caccgccgcc agcaaggggc ccagggaact
    22741 gccaaagttg ccgccgacct gaaatataga ttgcgccagg ccatgacgcc cgccggaagc
    22801 catacgggcc acgcgagaag attccggatg aaagaccgat gaaccggtac cgaccagcgc
    22861 cgctgccagc agaactgcgc cgaaactgcc cgccagcgcc agcagcacca gaccacttaa
    22921 ggtaaagcac atgccaatcg gcaacgacca cggcatcgga tatttatcgg tccagtagcc
    22981 gaccaccggt tgcagtagcg aagaggcgag ctggaaggtg agggttatca tgccaatctg
    23041 cataaatgtc agagaaaatt ctgactgaag cagtggataa atcgccagaa tcagcgattg
    23101 gatcatgtcg ttcagcagat gtgagaggct gatagcacct aaaataccaa acgatgttcg
    23161 ggccttagtc gttgacgcag ccgcgcccgc cacaggctgg gtttgttcac tcattgccat
    23221 aggaaagtca ctttttcagg gttgcgatgt aaagaatgat cttatttgtg attattaccc
    23281 gactaacata cctgtatgcg ccgcctgaag gaagtctcaa cacggaatac agaatttcta
    23341 atctggatgc agatttatct tcaccggacg cagacttgtt tatgatgtcg cgtcatacta
    23401 tttttcaaca cgttgaaatc aggtcaggga gagaagtatg aaattattgc agcggggcgt
    23461 ggcgttagca ctgttaacca catttacact ggcgagtgaa actgctctgg cgtacgagca
    23521 ggataaaacc tacaaaatta cagttctgca caccaatgat catcatgggc atttttggcg
    23581 caatgaatat ggtgaatatg gtctggcggc gcaaaaaacg ctggtggatg gtatccgcaa
    23641 agaggttgcg gctgaaggcg gtagcgtgct gctactttcc ggtggcgaca ttaacactgg
    23701 cgtgcccgag tccgacttac aggatgccga acctgatttt cgcggtatga atctggtggg
    23761 ctatgacgcg atggcgatcg gtaatcatga atttgataac ccgctcaccg tattacgcca
    23821 gcaggaaaag tgggctaagt tcccgttgct ttccgccaat atctaccaga aaagtaccgg
    23881 cgagcgcctg tttaaaccat gggcgctgtt taagcgtcag gatctgaaaa ttgccgttat
    23941 tggcctgacg acggatgaca cagccaaaat tggtaatccg gaatatttca ctgatatcga
    24001 atttcgtaag cccgctgatg aagcgaagct ggtgattcag gagctgcaac agacagaaaa
    24061 gccagacatt attatcgcgg cgacccatat ggggcattac gacaatggtg agcacggctc
    24121 taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct gccggatcgc tggcgatgat
    24181 cgtcggtggt cactcgcaag atccggtctg tatggcggca gaaaacaaaa aacaggtcga
    24241 ttacgtgccg ggtacgccat gcaaaccgga tcaacaaaac ggcatctgga ttgtgcaggc
    24301 gcatgagtgg ggcaaatacg tgggacgggc tgattttgag tttcgtaatg gcgaaatgaa
    24361 aatggttaac taccagctga ttccggtgaa cctgaagaag aaagtgacct gggaagacgg
    24421 gaaaagcgag cgcgtgctgt acacccctga aatcgctgaa aaccagcaaa tgatctcgct
    24481 gttatcaccg ttccagaaca aaggcaaagc gcagctggaa gtgaaaatag gcgaaaccaa
    24541 tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta cagaccaata tggggcggtt
    24601 gattctggca gcacaaatgg atcgcactgg tgccgacttt gcggtgatga gcggaggcgg
    24661 aattcgtgat tctatcgaag caggtgatat cagctataaa aacgtgctga aagtgcagcc
    24721 attcggcaat gtggtggtgt atgccgacat gaccggtaaa gaggtgattg attacctgac
    24781 agccgtcgca caaatgaagc cagattcagg tgcctacccg caatttgcca acgttagctt
    24841 tgtggcgaaa gacggcaaac tgaacgacct gaaaatcaaa ggcgaaccgg tcgatccggc
    24901 gaaaacttac cgtatggcga cattaaactt caatgccacc ggcggtgatg gctatccgcg
    24961 ccttgataac aaaccgggct atgtgaatac cggctttatt gatgccgaag tgctgaaagc
    25021 gtatatccag aaaagctcgc cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag
    25081 ctggcagtaa tgaaccccgt cgcggcgtca tgatatgacg ccgccattta tgaatattga
    25141 aatcttgcat cgtaatcgcg atgtgtcaaa ccaaatgcca tcagcatata attctgcgga
    25201 tcaccaggct gttcttcatc tgcttcaaac caaagtgtca cgcctgagcg attgacgtgg
    25261 attaagagtg tatggggcga ttgacggcgg atttgcagat cagcgccttc gttaacaagc
    25321 atgttaaata cacgcgtaac gttcaactca gcgacgttat tcaatattcc tttatcaaat
    25381 cggatcgctt tgcccaaatg gatttggctc agataatcgc accaaatcat atatagcttg
    25441 ttgttttcga aatgaaactc aacattacca aatagccaga tataaaaacc atttcccata
    25501 tcatttttag agtccggctc aacaaagttt tgttccaacc atgccattgt ttgccccagt
    25561 tcgacaaaat cgaattttcc gttaaagaca aaattgtgga agctaacatc aacaagatgg
    25621 tcaaatttca tctttcgctc ctttgtgaat tttttacatg cttcaaaagc cagcgtaatt
    25681 tacatcggtg caggcgactc tccagtgttg agcgaaaatt tgcgatgtcg acattggacc
    25741 gatctaccgc agcaaccccc gtgtattgtg ggatcggtga agggattttt tgtaactttc
    25801 ccagcgccag cagttgttga atgatcctgc taccgtcgtg gtaatcatcg taaatagcca
    25861 ctggcagagg ctgccaggcg ggggtcgatt tgataaatcc ggaaaaaaaa tacgggtcca
    25921 gatcgccgaa atcaaagccg taggcgaggg gaacttcaat attttgctct gtttttttat
    25981 tctcaaatgt acaatgaaaa ccgtgaaaac aatactgcca ctttcccatt ttgccttttc
    26041 gcttacggtt gcatttgaag ggggcaaagg taactagcgg gttattttcc gccatctcaa
    26101 cgttcagttg ttcagccaat gcaaatataa gctctgtcgc cagcttacga tattctttcg
    26161 cgcagtgttc gaaaaaatcc tgatggcggt taatctcttc aagcgatatt aatctggcga
    26221 tcctgataac cgggtcggca gaatgcatgc caacaacaaa ttcatcgagc tgtgctttca
    26281 gcaccagccc gaattcctca cgatacctct ctgcttcgct ggcaaaatgc agatgtaata
    26341 ccttctctct cgatgtttgc cgaagtaagg cgatatccgg tatctcatct gcacaagcga
    26401 tacttaatgt ctctgtaatc ctttccacat ctcatcttcc gtgtgcatca cttaatcgcg
    26461 acgggcgata tcagcaaatt tggcatcgag tatctttgcc aggtcgcttg ccgccagttc
    26521 gatatccagt ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc
    26581 gtcgataatc gttggcagac gttttttctg ccccagtggg ctaatccccc ctaccagata
    26641 ccccgtcgaa cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc
    26701 ttttgctact tttttaagat ccagttgacc tgcgaccggc gtaacagcca cggcaaggtg
    26761 tttcatatca ccgttcactg cgaccagcag cgttttgtag acctgatccg gattcaaacc
    26821 taattttttg acgacttcat cgccaaaatt ggtttcagca ggatcgtgct cgtaggtgtg
    26881 gatttgaaac gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt
    26941 cctgccttaa acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagag
//
LOCUS       DAOFSV010000052        25882 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.052, whole genome shotgun sequence.
ACCESSION   DAOFSV010000052 DAOFSV010000000
VERSION     DAOFSV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.052"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            233..4261
                     /gene="rpoB"
                     /locus_tag="QBH27_002323"
     CDS             233..4261
                     /gene="rpoB"
                     /locus_tag="QBH27_002323"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDQ7014626.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            4338..8561
                     /gene="rpoC"
                     /locus_tag="QBH27_002324"
     CDS             4338..8561
                     /gene="rpoC"
                     /locus_tag="QBH27_002324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDQ7014627.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(8803..9936)
                     /gene="thiH"
                     /locus_tag="QBH27_002325"
     CDS             complement(8803..9936)
                     /gene="thiH"
                     /locus_tag="QBH27_002325"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDQ7014628.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(9933..10703)
                     /gene="thiG"
                     /locus_tag="QBH27_002326"
     CDS             complement(9933..10703)
                     /gene="thiG"
                     /locus_tag="QBH27_002326"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDQ7014629.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(10705..10905)
                     /gene="thiS"
                     /locus_tag="QBH27_002327"
     CDS             complement(10705..10905)
                     /gene="thiS"
                     /locus_tag="QBH27_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDQ7014630.1"
                     /translation="MQILFNDQPMQCVAELTVHELLEQLEQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(10889..11644)
                     /gene="thiF"
                     /locus_tag="QBH27_002328"
     CDS             complement(10889..11644)
                     /gene="thiF"
                     /locus_tag="QBH27_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDQ7014631.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIRLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(11637..12272)
                     /gene="thiE"
                     /locus_tag="QBH27_002329"
     CDS             complement(11637..12272)
                     /gene="thiE"
                     /locus_tag="QBH27_002329"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDQ7014632.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(12272..14167)
                     /gene="thiC"
                     /locus_tag="QBH27_002330"
     CDS             complement(12272..14167)
                     /gene="thiC"
                     /locus_tag="QBH27_002330"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDQ7014633.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(14248..14348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14400..14876)
                     /gene="rsd"
                     /locus_tag="QBH27_002331"
     CDS             complement(14400..14876)
                     /gene="rsd"
                     /locus_tag="QBH27_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDQ7014634.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            14971..15744
                     /gene="nudC"
                     /locus_tag="QBH27_002332"
     CDS             14971..15744
                     /gene="nudC"
                     /locus_tag="QBH27_002332"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDQ7014635.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            15784..16848
                     /gene="hemE"
                     /locus_tag="QBH27_002333"
     CDS             15784..16848
                     /gene="hemE"
                     /locus_tag="QBH27_002333"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDQ7014636.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            16858..17529
                     /gene="nfi"
                     /locus_tag="QBH27_002334"
     CDS             16858..17529
                     /gene="nfi"
                     /locus_tag="QBH27_002334"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDQ7014637.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALEWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            17572..18162
                     /locus_tag="QBH27_002335"
     CDS             17572..18162
                     /locus_tag="QBH27_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDQ7014638.1"
                     /translation="MLQNPIHLRLERLASWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            18349..18621
                     /gene="hupA"
                     /locus_tag="QBH27_002336"
     CDS             18349..18621
                     /gene="hupA"
                     /locus_tag="QBH27_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDQ7014639.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            18634..19329
                     /locus_tag="QBH27_002337"
     CDS             18634..19329
                     /locus_tag="QBH27_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDQ7014640.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGQKQQLSNDQIDLYRYRAAQIREISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(19331..19750)
                     /gene="zraP"
                     /locus_tag="QBH27_002338"
     CDS             complement(19331..19750)
                     /gene="zraP"
                     /locus_tag="QBH27_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDQ7014641.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            19988..21364
                     /gene="zraS"
                     /locus_tag="QBH27_002339"
     CDS             19988..21364
                     /gene="zraS"
                     /locus_tag="QBH27_002339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDQ7014642.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGLHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            21361..22686
                     /gene="zraR"
                     /locus_tag="QBH27_002340"
     CDS             21361..22686
                     /gene="zraR"
                     /locus_tag="QBH27_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDQ7014643.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(22683..23972)
                     /gene="purD"
                     /locus_tag="QBH27_002341"
     CDS             complement(22683..23972)
                     /gene="purD"
                     /locus_tag="QBH27_002341"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDQ7014644.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(23984..25573)
                     /gene="purH"
                     /locus_tag="QBH27_002342"
     CDS             complement(23984..25573)
                     /gene="purH"
                     /locus_tag="QBH27_002342"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDQ7014645.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKCLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         5989 a         6827 c         7158 g         5908 t
ORIGIN      
        1 tagcgtttca cactgtttga ctactgctgt gcctttcaat gcttgtttct atcgacgact
       61 taatatactg cgacaggacg cccgttctgt gtaaatcgca atgaaatggt ttaagcgtga
      121 tagcaacagg cattgcggaa agtgttccat tttccggtca acaaaatagt gttgcacaaa
      181 ctgtccgctc aatggacaga tgggtcgact tgtcagcgag ctgaggaacc ctatggttta
      241 ctcctatacc gagaaaaaac gtattcgtaa ggattttggt aaacgtccac aagttctgga
      301 tgtaccttat ctcctttcta tccagcttga ctcgtttcag aaatttatcg agcaagatcc
      361 tgaagggcag tatggtctgg aagctgcttt ccgttccgta ttcccgattc agagctacag
      421 cggtaattcc gagctgcaat acgtcagcta ccgccttggc gaaccggtgt ttgacgtcca
      481 ggaatgtcaa atccgtggcg tgacctattc cgcaccgctg cgcgttaaac tgcgtctggt
      541 gatctatgag cgcgaagcgc cggaaggcac cgtaaaagac attaaagaac aagaagtcta
      601 catgggcgaa attccgctca tgacggacaa cggtaccttt gttatcaacg gtactgagcg
      661 tgttatcgtt tcccagctgc accgtagtcc gggcgtcttc tttgactccg acaaaggtaa
      721 aacccactct tcgggtaaag tgctgtataa cgcgcgcatc atcccttacc gtggttcctg
      781 gctggacttc gaattcgatc cgaaggacaa cctgttcgta cgtatcgacc gtcgccgtaa
      841 actgcctgcg accatcattc tgcgtgccct gaactacacc acagagcaga tcctcgacct
      901 gttctttgaa aaagttatct ttgaaatccg tgataacaag ctgcagatgg aactggtgcc
      961 ggaacgcctg cgtggtgaaa ccgcatcttt cgacatcgaa gctaacggta aagtgtacgt
     1021 agaaaaaggc cgccgtatta ctgcgcgcca cattcgccag ctggaaaaag acgacgtcaa
     1081 actgatcgaa gtcccggttg agtacatcgc aggtaaagtg gttgctaaag actatattga
     1141 tgagtctacc ggcgagctga tctgcgcagc gaacatggag ctgagcctgg atctgctggc
     1201 taagctgagc cagtctggtc acaagcgtat cgaaacgctg ttcaccaacg atctggatca
     1261 cggcccatat atctctgaaa ccttacgtgt cgacccaact aacgaccgtc tgagcgcact
     1321 ggtagaaatc taccgtatga tgcgccctgg cgagccgccg actcgtgaag cagctgaaag
     1381 cctgtttgag aacctgttct tctccgaaga ccgctatgac ctgtctgcgg ttggtcgtat
     1441 gaagttcaac cgttctctgc tgcgcgaaga aatcgaaggt tccggtatcc tgagcaaaga
     1501 cgacatcatt gatgttatga aaaagctcat cgatatccgt aacggtaaag gcgaagtcga
     1561 tgatatcgac cacctcggca accgtcgtat ccgttctgtt ggcgaaatgg cggaaaacca
     1621 gttccgcgtt ggcctggtac gtgtagagcg tgcggtgaaa gagcgtctgt ctctgggcga
     1681 tctggatacc ctgatgcctc aggatatgat caacgccaag ccgatttccg cagcagtgaa
     1741 agagttcttc ggttccagcc agctgtctca gtttatggac cagaacaacc cgctgtctga
     1801 gattacgcac aaacgtcgta tctcggcact cggcccaggc ggtctgaccc gtgaacgtgc
     1861 aggcttcgaa gttcgagacg tacacccgac tcactacggt cgcgtatgtc caatcgaaac
     1921 ccctgaaggt ccgaacatcg gtctgatcaa ctccctgtcc gtgtacgcac agactaacga
     1981 atacggcttc cttgagactc cgtatcgtaa agtgaccgac ggtgttgtaa ctgacgaaat
     2041 tcactatctg tctgctatcg aagaaggcaa ctacgttatc gcccaggcga actccaacct
     2101 ggatgaagaa ggccacttcg tagaagacct ggtaacttgc cgtagcaaag gcgaatccag
     2161 cttgttcagc cgcgaccagg ttgactacat ggacgtatcc acccagcagg tggtatccgt
     2221 cggtgcgtcc ctgatcccgt tcctggaaca cgatgacgcc aaccgtgcat tgatgggtgc
     2281 gaacatgcaa cgtcaggccg ttccgactct gcgtgctgat aagccgctgg ttggtacggg
     2341 tatggaacgt gctgttgccg ttgactctgg tgtaactgcg gttgctaaac gtggtggtgt
     2401 cgttcagtac gtggatgctt cccgtatcgt tatcaaagtt aacgaagacg agatgtatcc
     2461 gggtgaagca ggtatcgaca tctacaacct gaccaagtac acccgttcta accagaacac
     2521 ctgtatcaac cagatgccgt gtgtgtctct gggtgaaccg gtagaacgtg gcgacgtgct
     2581 ggcagacggt ccgtccaccg acctcggtga actggcgctt ggtcagaaca tgcgcgtagc
     2641 gttcatgccg tggaatggtt acaacttcga agactccatc ctcgtatccg agcgtgttgt
     2701 tcaggaagac cgtttcacca ccatccatat tcaggaactg gcgtgtgtgt cccgtgatac
     2761 caagctgggg ccggaagaga tcaccgctga catcccgaac gtgggtgaag ctgcgctctc
     2821 caaactggat gaatccggta tcgtttacat tggtgcggaa gtgaccggtg gcgacattct
     2881 ggttggtaag gtaacgccga aaggtgaaac tcagctgacc ccagaagaaa aactgctgcg
     2941 tgcgatcttc ggtgagaaag cgtctgacgt taaagactct tctctgcgcg taccaaacgg
     3001 tgtatccggt acggttatcg acgttcaggt ctttactcgc gatggcgtag aaaaagataa
     3061 acgtgcgctg gaaatcgaag aaatgcagct caaacaggcg aagaaagacc tgtctgaaga
     3121 actgcagatc ctcgaagcag gtctgttcag ccgtatccgt gctgtgctgg tagccggtgg
     3181 cgttgaagct gagaagctcg acaaactgcc gcgcgatcgc tggctggagc tgggcctgac
     3241 cgacgaagag aaacaaaatc agctggaaca gctggctgag cagtatgacg aactgaaaca
     3301 cgagttcgag aagaaactcg aagcgaaacg ccgcaaaatc acccagggcg acgatctggc
     3361 accgggcgtg ctgaagattg ttaaggtata tctggcggtt aaacgccgta tccagcctgg
     3421 tgacaagatg gcaggccgtc atggtaacaa gggtgtaatt tctaagatca acccgatcga
     3481 agatatgcct tacgatgaaa acggtactcc ggtagacatc gtactgaacc cgctgggcgt
     3541 accgtctcgt atgaacattg gtcagatcct cgaaacccac ctgggtatgg ctgcgaaagg
     3601 tatcggcgac aagatcaacg ccatgctgaa acagcagcaa gaagtcgcga aactgcgcga
     3661 attcatccag cgtgcgtacg atctgggcgc tgatgttcgt cagaaagttg acctgagtac
     3721 cttcagcgat gaagaagtta tgcgtctggc tgaaaacctg cgcaaaggta tgccaatcgc
     3781 aacgccggtc ttcgacggtg cgaaagaagc agaaattaaa gagctgctga aacttggcga
     3841 cctgccgact tctggtcaga tccgcctgta cgacggtcgc actggtgaac agttcgaacg
     3901 tccggtaacc gttggttaca tgtacatgct gaaactgaac cacctggtcg acgacaagat
     3961 gcacgcgcgt tccaccggtt cttacagcct ggttactcag cagccgctgg gtggtaaggc
     4021 acagttcggt ggtcagcgtt tcggggagat ggaagtgtgg gcgctggaag catacggcgc
     4081 agcatacacc ctgcaggaaa tgctcaccgt taagtctgat gacgtgaacg gtcgtactaa
     4141 gatgtataaa aacatcgtgg acggcaacca tcagatggag ccgggcatgc cagaatcctt
     4201 caacgtattg ttgaaagaga ttcgttcgct gggtatcaac atcgaactgg aagacgagta
     4261 attctcgctc aaacaggtca ctgctgtcgg ggtaaaaccc ggcagcggat tgtgctaact
     4321 ccgacgggag caaatccgtg aaagatttat taaagtttct gaaagcgcag actaaaaccg
     4381 aagagtttga tgcgatcaaa attgctctgg cttcgccaga catgatccgt tcatggtctt
     4441 tcggtgaagt taaaaagccg gaaaccatca actaccgtac gttcaaacca gaacgtgacg
     4501 gccttttctg cgcccgtatc tttgggccgg taaaagatta cgagtgcctg tgcggtaagt
     4561 acaagcgcct gaaacaccgt ggcgtcatct gtgagaagtg cggcgttgaa gtgacccaga
     4621 ctaaagtacg ccgtgagcgt atgggccaca tcgaactggc ttccccgact gcgcacatct
     4681 ggttcctgaa atcgctgccg tcccgtatcg gcctgctgct cgatatgccg ctgcgcgata
     4741 tcgaacgcgt actgtacttc gaatcctatg tggttatcga aggtggtatg accaacctgg
     4801 agcgtcagca gatcctgact gaagagcagt atctggacgc gctggaagag ttcggtgacg
     4861 aattcgacgc gaagatgggg gcggaagcaa tccaggccct gctgaagagc atggatctgg
     4921 agcaagagtg cgaacagctg cgtgaagagc tgaacgaaac caactccgaa accaagcgta
     4981 aaaagctgac caagcgtatc aaactgctgg aagcgttcgt tcagtctggt aacaaaccag
     5041 agtggatgat cctgaccgtt ctgccggtac tgccgccaga tctgcgtccg ctggttccgc
     5101 tggatggtgg tcgtttcgcg acttctgacc tgaacgatct gtatcgtcgc gtcattaacc
     5161 gtaacaaccg tctgaaacgt ctgctggacc tggctgcgcc ggatatcatc gtacgtaacg
     5221 aaaaacgtat gctgcaggaa gcggtagacg ccctgctgga taacggtcgt cgcggtcgtg
     5281 caatcaccgg ttctaacaag cgtcctctga aatctttggc cgacatgatc aaaggtaaac
     5341 agggtcgttt ccgtcagaac ctgctcggta agcgtgttga ctactccggt cgttctgtaa
     5401 tcaccgtagg tccatacctg cgtctgcatc agtgcggtct gccgaagaaa atggcgctgg
     5461 agctgttcaa accgttcatc tacggcaagc tggaactgcg tggtcttgct accaccatca
     5521 aagctgcgaa gaaaatggtt gagcgcgaag aagctgtcgt ttgggatatc ctggacgaag
     5581 ttatccgcga acacccggta ctgctgaacc gtgcaccgac tctgcaccgt ctgggtatcc
     5641 aggcatttga accggtactg atcgaaggta aagctatcca gctgcacccg ctggtttgtg
     5701 cggcatataa cgccgacttc gatggtgacc agatggccgt tcacgtaccg ctgacgctgg
     5761 aagcccagct ggaagcgcgt gcgctgatga tgtctaccaa caacatcctg tccccggcga
     5821 acggcgaacc aatcatcgtt ccgtctcagg acgttgtact gggtctgtac tacatgaccc
     5881 gcgactgtgt taacgccaaa ggcgaaggca tggtgctgac tggcccgaaa gaagcagaac
     5941 gtctgtatcg ctctggtctg gcttctctgc atgcgcgcgt taaagtgcgt atcaccgagt
     6001 atgaaaaaga tgctaacggt gaattagtgg cgaaaaccag cctgaaagac acgactgttg
     6061 gccgtgccat tctgtggatg attgtaccga aaggtctgcc ttacaccatc gtcaaccagg
     6121 cgctgggtaa aaaagcaatc tccaaaatgc tgaacacctg ctaccgcatt ctcggtctga
     6181 aaccgaccgt tatttttgcg gaccagatca tgtataccgg ctttgcatat gcagcgcgtt
     6241 ctggtgcatc tgttggtatc gatgacatgg tcatcccgga gaagaaacac gaaatcatct
     6301 ccgaggcaga agcagaagtt gctgaaattc aggagcagtt ccagtctggt ctggtaactg
     6361 cgggcgaacg ctacaacaaa gttatcgata tctgggctgc ggcgaacgat cgtgtatcca
     6421 aagcgatgat ggataacctg caaactgaaa ccgttattaa ccgtgacggt caggaagaga
     6481 agcaggtttc cttcaacagc atctacatga tggccgactc cggtgcgcgt ggttctgcgg
     6541 cacagattcg tcagcttgct ggtatgcgtg gtctgatggc gaagccggat ggctccatca
     6601 tcgaaacgcc aatcaccgcg aacttccgtg aaggtctgaa cgtactccag tacttcatct
     6661 ccacccacgg tgctcgtaaa ggtctggcgg ataccgcact gaaaacggcg aactccggtt
     6721 acctgactcg tcgtctggtt gacgtggcgc aggacctggt ggttaccgaa gacgattgtg
     6781 gtacccatga aggtatcatg atgactccgg ttatcgaggg tggtgacgtt aaagagccgc
     6841 tgcgcgatcg cgtattgggt cgtgtaactg ctgaagacgt tctgaagccg ggtactgctg
     6901 atatcctcgt tccgcgcaac acgctgctgc acgaacagtg gtgtgacctg ctggaagaga
     6961 actctgtcga cgcggttaaa gtacgttctg ttgtatcttg tgacaccgac tttggtgtat
     7021 gtgcgcactg ctacggtcgt gacctggcgc gtggccacat catcaataaa ggtgaagcaa
     7081 tcggtgttat cgcggcacag tccatcggtg aaccgggtac acagctgacc atgcgtacgt
     7141 tccacatcgg tggtgcggca tctcgtgcgg ctgctgaatc cagcatccag gtgaaaaaca
     7201 aaggtagcat caagctcagc aacgtgaagt cggttgtgaa ctccagcggt aaactggtta
     7261 tcacttcccg taacaccgaa ctgaaactga tcgacgaatt cggtcgtacc aaagaaagct
     7321 acaaagtacc ttacggtgcg gtactggcga aaggcgatgg cgaacaggtt gctggcggcg
     7381 aaaccgttgc aaactgggac ccgcacacca tgccggttat caccgaagta agcggttttg
     7441 tacgctttac tgacatgatc gacggccaga ccattactcg tcagaccgac gaattgaccg
     7501 gtctgtcttc gctggtggtt ctggattccg cagaacgtac cgcaggtggt aaagatctgc
     7561 gtccggcact gaaaatcgtt gatgctcagg gtaacgacgt tctgatcccg ggtaccgata
     7621 tgcctgcgca gtacttcctg ccgggtaaag cgattgttca gctggaagat ggcgtacaga
     7681 tcagctctgg tgacaccctg gcgcgtattc cgcaggaatc cggcggtacc aaggacatca
     7741 ccggtggtct gccgcgcgtt gcggacctgt tcgaagcacg tcgtccgaaa gagccggcaa
     7801 tcctggctga aatcagcggt atcgtttcct tcggtaaaga aaccaaaggt aaacgtcgtc
     7861 tggttatcac cccggtagac ggtagcgatc cgtacgaaga gatgattccg aaatggcgtc
     7921 agctcaacgt gttcgaaggt gaacgtgtag aacgtggtga cgtaatttcc gacggtccgg
     7981 aagcgccgca cgacattctg cgtctgcgtg gtgttcatgc tgtgactcgt tacatcgtta
     8041 acgaagtaca ggacgtatac cgtctgcagg gcgttaagat taacgataaa cacatcgaag
     8101 ttatcgttcg tcagatgctg cgtaaagcta ccatcgttaa cgcgggcagc tccgacttcc
     8161 tggaaggtga acaggttgaa tactctcgcg tcaagatcgc aaaccgcgaa ctggaagcga
     8221 acggtaaagt gggtgcaact tactcccgcg atctgctggg tattaccaaa gcgtctctgg
     8281 caaccgagtc cttcatctcc gcggcatcgt tccaggagac cactcgcgtg ctgaccgaag
     8341 cagccgttgc gggcaaacgc gacgaactgc gcggcctgaa agagaacgtt atcgtgggtc
     8401 gtctgatccc ggcaggtacc ggttacgcgt accaccagga tcgtatgcgt cgccgtgctg
     8461 cgggtgaagc tccggctgca ccgcaggtga ctgcagaaga cgcatctgcc agcctggcag
     8521 aactgctgaa cgcaggtctg ggcggttctg ataacgagta atcgttaatc cgcaaataac
     8581 gtaaaaaccc gcttcggcgg gtttttttat gggtgttatt cagagggcaa agtcttcacg
     8641 ggaaatatta agaaaggcag ggcgaaacga ggaagaagct tttcgccctg gacaacattc
     8701 ctgctgacgg cactaccata aaaaaacaca acgctttcaa caaccttgcg tttttcatct
     8761 tccgtagcgg ctcggaataa atttacgttc ttacattctg tctcatggcc tttgcgatgg
     8821 gcgtcccaga tagctgtccc agtctttcca taccggctgc aaaccctgag cggttaacgc
     8881 ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag aactgttcca gctcggggtg
     8941 gttgtcggca tagccacctg gctgcgtttt cgaaaaagcg ctgacgttat taattgccag
     9001 cggaatcacg cgatcgcgaa accacggtga ttcccgcgtg gagagtgaca gttcaatctc
     9061 cggtgcaagc agccggaaag cgcagatggc ttgcactaac tggcgttcat ccataatcga
     9121 cgcaggctca atgccgccag tacatggacg caggcgtggg aaggagacag agtagcggct
     9181 ttgccagtaa tgctgttgca gccatagcaa atgttcggca accatatagc agtcaactcg
     9241 ccagctgtcg gaaaggccga ttagcgcgcc gaggcctatc ttatcaatcc ccgcacgccc
     9301 tagccgatcc ggcgtttcca gccgccagaa gaagtcctgt tttttacctt tcagatgatg
     9361 atgggcataa gtcgcctcat gatatgtctc ctgataaacc attactccat cgaggccaag
     9421 ctgttttaac tcggcatatt ccgcctccgc cagcggttgc acttccatct gtagtgaaga
     9481 gaactgttca cgcagggcag ggagatgacg acgaaagtaa tccatcccca ctttcgcctg
     9541 atgttcacca gtgactaata gcagatgttc aaagcccatc tcccgtatag cggcgctttc
     9601 cctggcaata tccgcttcat ccagcgtttt gcgcttgatg cggttgctca tagaaaatcc
     9661 gcaataagta cagtcgttag cgcaaagatt ggaaagataa agcgggacgt agaagctgac
     9721 gacgttgcca aatcgctgac gagtcagacg ttgcgctcgc tgggccagtg gttccagata
     9781 gccactggcg gcgggtgata gcaatgccat catgtcgtcg cgggtgagtt gcgaggcatt
     9841 tagcgcccgc tctacgtcag cagccgtttt gccgttgata cgcaggtgga tgtcatccca
     9901 gtccagttgt cgccagcgat cgctgaaggt tttcatgccg atgcctccag aaatccagtc
     9961 agcgggctgg tggcatgagc aaaatgactg cggctgcccg gtccggactg acgtgccagc
    10021 aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt tgacgggatc gtccgcgacg
    10081 gcaatcgccg tattcactaa caccgcgtcg gcccccattt ccagcgcctg cgcggcatgg
    10141 ctgggaacgc cgatgccagc atcgacaacc accggaacgg tggcctgctg gataataatc
    10201 tccagcatgg cgcgggtttc cagtccctga ttcgagccaa tcggcgcgcc gagcggcatc
    10261 accgctgcac agccgacttc ttccagacgt ttacacaata ccggatcggc cccgcagtaa
    10321 ggcagcacga caaatccctg ttgtaccagc gtttcggcgg ctttcagggt ttcgatggga
    10381 tcgggcaaca gccagcgggc gtcagggtga atctccaatt ttagccagtt tgtgcctaac
    10441 gcttcacgtg ccagatgggc ggcgaaaatg gcttcttccg cggttttcgc cccggaagta
    10501 ttcggcagca gggccacacc cgccgcgata agcggttcga ggatagcgtc gttgtgctgg
    10561 cgcaagtcga cacgtttcat cgccagcgtc accagttggc tgccggaagc gcgaatagac
    10621 tccaccatca gttgtgaaga ggcgaatttt ccggtgccag taaacagatg tgaatcaaac
    10681 gttttgtccg caatatgtaa catctcaacc ccctgcaata acttgaaaaa gcaggatctg
    10741 gtcgccatcc tgcacgatat gttgcgccca ttgttcccgc gggacgattt gctgattaat
    10801 cgccagtgcc gcacctgctt gttgttgttc gagttgctcc agcagttcgt gaacagttag
    10861 ttcggcgaca cactgcatcg gttgatcgtt aaacaggatc tgcattgcat cctccacata
    10921 ccgggcaacc gctggcgcgg cgcaacgtca gactgcgcca ctggctcgat ttaccgtcga
    10981 acagtcggag ttctcccgca ggcgtctcta taccacttaa taacttaatc gcttccagtg
    11041 cctgtaaagt gcccataacc ccgaccaccg ggccaaccac gcccgccgtg cggcagttgc
    11101 gttctggctc ctggttatct ggccacaggc agcggtaaca tccctgctcc cagggcggcg
    11161 tcagtaccat caactgaccg ccaaatccga ccgcgctggc ggtgataagc ggcgtgttga
    11221 gtgccacgca ggtggcatta atctcctggc gagtcgccat attgtcagta cagtcgagca
    11281 ccacatcggc ttgtgcaacc gcatctttta acgtctcacc cgttaaccgt tgttgtaatg
    11341 ccatcagtcg aatatcggga ttcaactgtg tcagtcgctg ttggctgacc tgtgatttcg
    11401 ggcgatcgat atcttcagtg gtaaagagga tttgtcgttg cagattgctt aaatgcacat
    11461 cgtcgtcatc tgccagtacc agcgtcccga cgccagcccc cgccaggtat agcgcagcag
    11521 gtgtacccag cccgccaaga ccaataatca gcacctggct gtcgagcagt ttttgctgcc
    11581 cgtccagagc gatatcgtcg agcaggattt ggcggctata acgcataaag tcacggtcat
    11641 tcatcgccaa ctcctgcaat ttccagcagc tgtgccgttg ccaaacgcca gtctgcggct
    11701 tgagtaatgg cgctgacgac ggcgatactg ccgacacccg ttgctatcac cgcaggcgcg
    11761 cgcgccagac tgataccgcc aatcgccacg gtgggataat ccgccaggcg ctcaacatgc
    11821 cgtgccagct gttccagtcc ctgcggtgca gaaggcatct gtttggtttg tgtcggaaac
    11881 acatgtccca gcgcgatata agaaggacgt gccgccagcg cgacgtcgat ctccatatcg
    11941 tcatgagtcg aaacgcccag ccgcaggccc gctgcacgga tggcattgag atcggtggct
    12001 tgcaaatctt cctgccccag atggacgcca tacgcctgat gcttaatcgc cagccgccag
    12061 taatcgttga taaacaatcg cgcgttatag cggcggccca gcgcaattgc cgccaccaca
    12121 tcggcttcta cctcttcatc gcgctgatct ttgatgcgta gctggagagt acgtacgcct
    12181 gcatccaaca gacgttcgat ccactgtacg ctgtcaacca ccgggtacag tcctaaacga
    12241 aagggtacag gaggaaactc aggctgatac atcacgcttc ctccttacgc aggtagattt
    12301 ctccgcctct ggcgcggaag ttctccgaca tatccgccat tcccacttca atggtttgcg
    12361 cggcggcgta atcacgcact tcctggctga ttttcatcga gcagaatttc ggtccacaca
    12421 tggagcaaaa atgggcgact ttaccggact cttgcggcag ggtttcatcg tgataagcgc
    12481 gggcagtaaa cgggtcgagg gccagattaa actggtcttc ccagcgaaat tcgaagcggg
    12541 ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg ccctttcgcc aggtcggcgg
    12601 cgtgagcggc aatcttatag gtgataagcc cctgtttaac atcttcttta tttggcaggc
    12661 ccagatgctc tttcggcgtt acgtagcaga gcatcgcgca gccaaaccag ccaatcatcg
    12721 ccgcgccaat ccctgacgtg aagtggtcat agcccggcgc gatatcggta gttagcggcc
    12781 ccagagtgta aaacggcgct tcgtggcagt gctctaactc ctcggtcata ttgcggcgaa
    12841 tcatctgcat cggcacgtgg cctgggcctt caatcatcac ctgcacgtca tattcccagg
    12901 cgattttggt cagttcgccc agcgtatgca gctcggcaaa ctgtgcttca tcgttggcgt
    12961 cctgaataga accggggcgc aggccgtcgc ccagcgacag cgaaacgtca taagcagcac
    13021 agatttcaca aatttcgcgg aagtgttgat agaggaaatt ttcctgatga tgggagaggc
    13081 accatttcgc cataatcgaa ccgccgcgag agacgatacc ggtcaggcgt ttcgcggtca
    13141 tcggcacgta gcgcagcagc acccccgcat ggatagtgaa gtaatccaca ccttgctcgg
    13201 cttgttccag cagggtgtcg cggaagactt cccaggtaag atcttcagcg atcccgttca
    13261 ccttctccag tgcctggtag atcggcactg taccgatcgg caccgggctg ttacgcaaaa
    13321 tccactcgcg ggtttcgtga atatagcgac cagtggaaag atccatcacg gtatccgctc
    13381 cccagcgcgt ggaccatacc agtttttcca cttcttcttc gatggaagaa gtgaccgccg
    13441 agttgccgat attggcgtta acttttacca ggaagttacg gccaataatc atcggctccg
    13501 attccggatg attaatgttg gccgggataa tcgcacgtcc ggcagcaact tcatcacgga
    13561 caaattccgc agtgatattt tccggcagac gtgcgccaaa gctcattccc ggatgctggt
    13621 ggcgtaaaac ttcgctacgt atgcgctcgc gacccatatt ctcgcgaatg gcgatgaatt
    13681 ccatttctgg cgtgatgata cccttgcggg cgtagtgcag ttgggtgacg cggcgtcctg
    13741 ctttggcgcg ttttggcgtt aatacgccgc taaaacgcag ctcgtcaagg ccatcatctg
    13801 ccagccgcgc tttagtgtaa tcggaactgc gtacggtaag ttcttcggta tcgccgcgcg
    13861 catcgatcca cggctggcgt agttttgcca gcccttgctg cacgttaatg gcaatctgcg
    13921 gatcgccata cgggccggag gtgtcgtaga ccggaatcgc ttcgttttct tcgtactgcg
    13981 gctgttcttt gctgccgcca attagcgtcg ggctaagctg gatctcacgc atcggcacgc
    14041 gcacgccggg gtgtgtgcca gtgatgtaaa tgcgttttga gttgggaaag gcggagcctt
    14101 ccagggtgtc gataaaatgt tgggcctggg cgcgctgttc gcggcgggtc agttttgttg
    14161 cagacatagc tcattccaaa aagttaagga cgtggcttgt cagacgacgg atgaagcaag
    14221 agacgatcgc cgcaaaggcg atgcgatagc agattaactc ttgttccctt cgcaggtatt
    14281 agcctgatca ggttccgcgg atcccgaata aacggtctca gccagttaag gcactccgac
    14341 aagaaattag ccccacaaac ggggcgttga atgtaaatta cgcgttaaca gcgcagaact
    14401 caagcaggat atttgacgcg ggcggcgtca agcaccagta gaatcagctt atcttccagc
    14461 acaaagcgcg cttccagtgc ctcgccgatg tcagataaaa cctgttgaaa ctcaaggtaa
    14521 ttatcatgat cgatggcggt ttccaggctg gaatcgtagt catccataat ctgttgagta
    14581 ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt gccttccagc
    14641 ttatgaagaa tgcgctcata aatactgaaa tgtccggcag acaagtaatc gaccaggctc
    14701 tgacaaaaat catcaagggc tttttcgttt agcctcatgt acgattcttt gccaggctta
    14761 atgccaacca gattgtagta agccacgagc agatgcttac gtacatgtag ccagcgatca
    14821 accagtttgt tacttcctct gacgcgctcc gtcaggttat cgagctggtt aagcatgatt
    14881 gactccgcaa gtttgtattc aaaaactgct ctgtgagaaa tgtaaaaacc atgttaaaca
    14941 tgccagtgat gcaaaggtag tgcaagagct atggatcgta taattgaaaa attagatcac
    15001 ggctggtggg tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga attgccatat
    15061 ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg cgaatggcag
    15121 ggggaacctg tttggttagt acaacagcag cggcgtcacg atatggggtc ggtacgtcag
    15181 gtcattgatc tcgatgttgg gctgtttcaa ctggccggac gtggcgtgca actggcggag
    15241 ttttaccgat cgcataaata ctgtggttac tgcgggcatg aaatgtatcc aagcaaaacc
    15301 gaatgggcga tgctgtgcag ccattgccgt gagcgttact acccgcaaat cgccccctgc
    15361 attattgttg ccatccgtcg cgatgattcg atcctcctcg cccagcatac ccgccatcgt
    15421 aacggtgtcc atacagtact tgccggattc gtcgaagtgg gtgaaaccct cgagcaggca
    15481 gtcgcgcggg aagtgatgga agagagcgga attaaagtta aaaacttgcg ttacgtgacc
    15541 tcccaaccgt ggccgtttcc tcagtcgtta atgaccgcgt ttatggcgga atatgacagc
    15601 ggcgagatcg tgatcgaccc gaaagaattg cttgaggcga actggtatcg ctatgacgat
    15661 ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga tacggtggcg
    15721 atgtgtcggg cagagtatga gtgatgatac actgaccgcc tgacgcacta aggaacagcc
    15781 aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca gcccgttgat
    15841 gtcactccag tatggatgat gcgccaggcg ggtcgctatc taccggaata taaagccacg
    15901 cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaatg ccgagctggc gtgcgaagtg
    15961 actttgcaac cgctgcgtcg ctatccgctg gatgcggcga tcctcttttc cgatatcctc
    16021 accgtgccag acgcgatggg tttagggctc tattttgaag ccggagaagg tccgcgtttt
    16081 acctcgccag tcacctgcaa agccgatgtc gataaactgc caattccgga cccggaagat
    16141 gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc gcgaactgaa aggcgaagtg
    16201 ccgctgattg gtttttccgg cagcccgtgg acgctggcga cctacatggt ggaaggcggc
    16261 agcagcaaag cgttcaccgt gatcaaaaaa atgatgtatg ccgatccaca ggcgctgcac
    16321 gcgctactcg ataaactggc gaaaagcgtc actttgtatc tgaatgcgca gattaaagcc
    16381 ggtgctcagg cagtgatgat tttcgacacc tggggcggcg tgcttaccgg gcgcgattat
    16441 caacagttct cgctctatta catgcataaa attgttgatg gtttactgcg tgaaaacgac
    16501 ggtcgccgcg taccggtcac gctgtttacc aaaggtggcg gacagtggct ggaagcgatg
    16561 gcagaaaccg gttgcgatgc gctgggcctc gactggacaa cggacatcgc cgatgcgcgc
    16621 cgtcgcgtgg gcaataaagt cgcgctgcag ggtaatatgg acccgtcgat gctgtatgcg
    16681 ccgcctgccc gcattgaaga agaagtggcg tctatacttg ccggtttcgg tcacggcgaa
    16741 ggccatgtct ttaaccttgg tcacggcatt catcaggatg tgccgccaga acatgctggc
    16801 gtgtttgtgg aggcagtgca tcgcttgtct gaacagtatc accgctaagg agcgattatg
    16861 gatctcgcgt cattacgcgc tcaacaaatt gaactggctt cttctgtgat ccgcgaggat
    16921 cgactcgata aagatccgcc ggatctgatc gccggagccg atgtcggatt tgagcagggc
    16981 ggagaagtga cgcgagcggc gatggtgctg ctgaaatacc cctcgcttga gctggtggag
    17041 tataaagtgg cccgcatcgc caccaccatg ccttacattc caggttttct ctcgttccgc
    17101 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    17161 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tagccagcca ttttggctta
    17221 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    17281 ctctccagcg aaccgggcgc gctggcccct ctgatggata aaggcgagca gctggcctgg
    17341 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    17401 gtggacagcg cgctggagtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    17461 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    17521 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    17581 aacccaattc atctgcgtct ggagcgcctg gcaagctggc agcatgtcac ttttatggcc
    17641 tgcttatgcg aacgcatgta ccccaattac gccgtattct gtcagcaaac cggcttcggc
    17701 gatgggcaga tttaccgccg cattctcgat ctcatttggg aaacgctgac cgtcaaagat
    17761 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    17821 gatttcgatc tgtacggcgt ttatccggca atcgatgctt gcgtggcgtt aagtgaactg
    17881 gttcattcgc gtttaagtgg cgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    17941 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatgag cgatgaagag
    18001 ctcaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    18061 ttagctgagt gtgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    18121 gaggcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    18181 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    18241 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    18301 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    18361 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    18421 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    18481 gttggtttcg gtaccttcaa agtgaaccac cgcgctgagc gtactggccg caacccgcag
    18541 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    18601 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    18661 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    18721 gcctgtagtc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    18781 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatcttct tgccgtgttt
    18841 agcccgtggc gcagtggcga tacgacgacg cgagagtatc gctggcaggg cgataacctc
    18901 acgcttatca atatcaacgt ttacagcaaa ccgccggtaa acatccgggc gcgttttgac
    18961 gatcgcggtg acttgagctt tatgcaacgt gaatccgacg ggcaaaagca gcagctttcc
    19021 aatgatcaga tcgatttata ccgttatcgt gccgcgcaga tccgcgagat tagcgatgcc
    19081 ttacgccagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga gcagactgtg
    19141 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    19201 gagcgccgcc agagccgttc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    19261 ggttcgcaat tactgttggt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    19321 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtaac
    19381 ccattccggt accgcgcgga atacccgctt cagccatcgc aatatctcgt ttcacccgta
    19441 actcatctaa cgactgacgc aaattctcca tctctttggc gaccgcgtta attttgctgc
    19501 tatccggtgg gttcgcggct aacagggcat tgtattcata acgctttgtc accagttgct
    19561 gttgcagtgc gctgctttga gcgtaaaagt cattatggat tttctgccac gctgtttgtt
    19621 gttcgctggt caaaggcgcg gcattttgct gccacatacc gtgtccgccg tgagcaaatg
    19681 ccgatgtcga tcccatcgcc attgctgaaa gcgccatcat taccagggca attttcgtgt
    19741 tccgtttcat ggttaatcct ccagtggttg tcttacttcg ggtattgcat ctttcgtgcc
    19801 aacggcgaaa cgctgatatg acgggtaatc tggcatgata aacgagtaaa aatgactcgc
    19861 ctgttgtggg tagcgagtca tttttactca ttgaaactta agcctttgtg ttacagcgca
    19921 gggtaagcgc tgataaaaga tggcatgatt tctgctgtca gaaagggatg ggcaggcaaa
    19981 gaagaagatg cgttttatgc aacgttctaa agactcctta gctaaatggt taagcgcgat
    20041 cctccccgtg gtcattgttg ggctggtggg gctgtttgcg gtgacagtga ttcgcgatta
    20101 tgggcgcgag actgccgccg ccagacaaac gctgctggaa aaaggcagtg tacttatccg
    20161 tgctctcgaa tccggctcgc gcgtcggaat ggggatgcgc atgcatcatg cgcagcagca
    20221 ggccttactg gaagaaatgg ccgggcagcc tggagtacgt tggtttgcgg tcacggatga
    20281 acaaggaaca atcgtgatgc atagcaactc cggcatggtg ggaaaacagc tttattcccc
    20341 gcaggaaatg cagcagttac atccgggaga tgaagaagcg tggcggcgga tcgatagcgc
    20401 agacggcgag cctgttctgg aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat
    20461 gcaccggatg cgccatatgc agcagtatgc cgcgacacca caagcaattt ttatcgcttt
    20521 cgacgccagt aacattgtga gtgccgaaga tcgtgagcag agaaacaccc tgattatcct
    20581 cttcgccctg gcgacggtct tgctggcaag cgtgttgtca ttcttctggt atcgccgata
    20641 tctgcgctcg ggccagctgt tgcaggatga aatgaagcgc aaagagaagc tggtggcact
    20701 ggggcatctt gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg
    20761 gctggcgaaa tactttgccg aacgcgcgcc agcaggggga gaagcgcatc aattggcgca
    20821 ggtgatggcg aaagaagccg accgtttaaa ccgcgtggta agcgagctgc tggaactggt
    20881 taagccaacg catctggctt tgcaggcggt ggagctcaac acgctgatta gccactcatt
    20941 acagctggta agccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga
    21001 cacattaccg gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta
    21061 tctcaacgct attcaggcga ttggtctgca tggcgtgatt agcgtgacgg ccagcgaaag
    21121 cggcgcgggc gtgaaaatca gcgttaccga cagcggtaag ggaattgcgg cggatcagct
    21181 tgaagccatc ttcactccgt acttcaccac caaagccgaa ggcaccggac tggggctggc
    21241 ggtcgtgcat aatattgttg aacaacacgg tggtacaatt caggttgcaa gccaggaggg
    21301 gaaaggcgca acgttcaccc tctggcttcc ggtcaatatt acgcgtaagg acccacaagg
    21361 atgacgcacg ataatatcga tattctggtg gtggatgatg acattagcca ctgcactatt
    21421 ttgcaggctt tactgcgcgg ctggggctat aacgtcgcgc tggcgaacag cgggcgacag
    21481 gcgctggagc aggtgcggga acaggttttc gatcttgtgc tttgcgatgt gcgaatggca
    21541 gagatggacg gcatcgccac gctaaaagag atcaaagcgt taaatccggc gattccggta
    21601 ctgattatga ccgcgtactc cagcgtcgag acggcggtag aggcactgaa aaccggggcg
    21661 ctggattacc tcatcaagcc gctggatttc gataacctgc aggcaacgct ggaaaaggcg
    21721 ctcgcgcata cgcacagtat cgacgctgaa acgcctgcgg tgtctgccag ccagttcggt
    21781 atggtcggta aaagcccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca
    21841 tcggaagcca cggtgctgat ccacggcgat tccggtaccg gtaaagagct ggtcgccagg
    21901 gcgattcacg ccagtagcgc acgtagcgaa aaaccgctgg taacgctcaa ctgtgcggca
    21961 ctcaacgaat ccttgctgga atctgaattg ttcggtcatg aaaaaggggc gtttaccgga
    22021 gccgacaaac ggcgagaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat
    22081 gagattggcg atatctcgcc gatgatgcag gttcgtctgc tgcgtgcgat tcaggagcgc
    22141 gaagtgcaac gtgtcggcag caaccagact atctcggttg atgtccggct gattgcggcg
    22201 acccatcgcg atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat
    22261 cggctgaacg tggtggcgat tgaagtacca tcgctgcggc aacggcggga agatattcct
    22321 ctgctggctg gccattttct tcagcgcttt gccgaacgta atcgcaaggc ggtaaaaggc
    22381 tttacgcccc aggcgatgga tctgctgatt cattacgact ggccgggaaa tattcgtgaa
    22441 ctggaaaacg cggtggaacg ggcggtggtg ctgctgaccg gggaatatat ttccgaacgc
    22501 gagctgccgc tggcgattgc cagtacgccg atcccgctgg tacaaagtca ggatattcag
    22561 ccgctggtgg aagtcgaaaa agaggtgatt ctggcggcgc tggagaaaac gggcggcaac
    22621 aaaaccgaag ccgcccgcca gttagggatc acgcgcaaaa cgctattggc aaaactgtcg
    22681 cgttagttct gctcgcgttc gatagcgcgc cagccgatat ctttccggca gaagcagtcg
    22741 tcccaatgga tatcggtcat taaggcataa gcgcgtttct gcgcttctgc cacggtatga
    22801 cccagcgcgg taacgcacag tacgcgcccg ccgctggtca ctacctgctc gtcatccgcc
    22861 agtttcgtgc ccgcgtggaa cactttgccg tctgccactt cttccagcgg caggccgtgg
    22921 atcacatcgc cggtgcggta atcgcccgga tatccacccg cagccatcac cacgccgaga
    22981 gaagcgcgtt catcccactc ggacgttttc tcgtccagct tgccttcaca ggccgccagg
    23041 cagagctcaa ccagatcgga cttcatgcgc agcataatcg gctgggtttc cggatcgcca
    23101 aagcggcagt taaattcaat aacctttgga ttaccctgtt tgtcgatcat cagacccgca
    23161 tagagaaaac cggtgtaggt gttgccttcc gccgccatgc ctttcacggt tggccagatg
    23221 atacgttcca tggtgcgctg atgaacttca tcggtcacta ccggcgcagg agagtaagcc
    23281 cccattccgc cggtgtttgg cccggtatct ttatcgccta cgcgtttgtg atcctggctg
    23341 gtggccatcg gtagcacatg ctcgccgtcc accatcacga taaagctcgc ttcttcaccg
    23401 tcgaggaact cttcgataac aatacgatga cccgcgtcgc caaacgcgtt gcccgccagc
    23461 atatcgtgaa cagctgcttc agcttcttcc agcgtcatcg ccacgataac gcctttcccg
    23521 gctgccagac cgtcagcttt aatgacgatt ggcgcgcctt tctcacgcag atatgccagc
    23581 gcaggttcta cctcggtgaa gttctggtat tccgctgtgg gaatgttatg gcgggccaga
    23641 aagtctttgg tgaatgcttt agaaccttcc agttgtgcag cacctgcggt tgggccgaag
    23701 attttcagcc cggcggcgcg gaaggtatcg actacgcctt tcaccagcgg cgcttccggg
    23761 ccgacgatgg ttagatctac cttttcgttt tgtgcgaaat ccagcagcgc cgggatatcg
    23821 gttacgccga tagcgacgtt ctgtagcgcg ggttccagcg cagtgcctgc attgcccgga
    23881 gcaacaaaaa cagtttcaac cagcggcgac tgagccgctt tccaggccag cgcgtgctcg
    23941 cgcccgccgt taccgatcac taatactttc atctgtcgct ccattaatgg cggaagtggc
    24001 gcatgtcggt gaagagcatc gcaataccgt gctcgtcggc ggcggcaatt acttcgtcat
    24061 cacggataga accgccaggc tggattacgc aggtcacgcc cgcagcggct gcggcatcaa
    24121 taccgtcgcg gaacgggaag aacgcgtcag aagccattga cgaacctttc acttccaaac
    24181 cttcatcggc agctttaata ccggcgattt tcgcggagta cacgcggctc atctggcctg
    24241 cgccaatacc gatggtcata ttgtttttgg catagacgat agcgttggat ttcacgaact
    24301 tcgccacttt ccagcagaac agcgcatcac gaagttcctg ttcagtcggc tgacgtttgg
    24361 tgacgacgcg cagttcttcc gcaccgacca tccccagatc gcgatcctga accagtagac
    24421 cgccgttcac gcgtttgaaa tcaagacccg gaatacgttc gccccactga ccgcaggtca
    24481 gaacgcgtac gttctgcttg gcggcggtga ttttcagggc ttcttcgctg gcggacggcg
    24541 caataatcac ttcgacgaac tgacgagaaa tgatggcctg cgcggtttca gcatccagtt
    24601 cgcggttaaa ggcaatgatg ccgccgaatg cggaggttgg gtcggttttg tacgcgcgat
    24661 cgtaagcatc aagaatggaa ttgccgatag ccacgccgca agggttggcg tgcttcacaa
    24721 tcacacatgc cggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga
    24781 tgttgttata agagagagct ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt
    24841 tcacattctc ttctatatag aaggcagcct gctggtggct gttctcgccg taacgcatat
    24901 cctgcttctt aatgaagttc aggttcagcg tgcgtgggaa gcgaccggcg gcttctttgc
    24961 tttcaccgtg gtaagccgga accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg
    25021 cggcagtgtg ttcgaaggct ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc
    25081 cttcgttggc atccatctct ttaataatgg cgtcgtagtc gctgctcttc accacgattg
    25141 cgacatcttt atggttcttg gcggcggagc gcaccatcgt tgggccgccg atatcgatgt
    25201 tctctaccgc atcttccagc gagcagcctt cacgagcgac ggtctgggcg aacggataca
    25261 ggttaacaac caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct
    25321 ggccgcgacg gcccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat
    25381 ccatcatctc cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga catttttctg
    25441 ctaacagacg ggcagtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct
    25501 gggcgaattc gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac
    25561 gacgttgttg catggtaaat cccctggatt tgactattac agagagcgtt agctgaattt
    25621 ttcgcgaaaa actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta
    25681 acgaaaacgt ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc acaattttga
    25741 cagtggctac cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgtg
    25801 taaaaaggta taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttctgcaat
    25861 ttttctattg cggcctgcgg ag
//
LOCUS       DAOFSV010000053        24974 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.053, whole genome shotgun sequence.
ACCESSION   DAOFSV010000053 DAOFSV010000000
VERSION     DAOFSV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.053"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            450..1568
                     /gene="hyaA"
                     /locus_tag="QBH27_002343"
     CDS             450..1568
                     /gene="hyaA"
                     /locus_tag="QBH27_002343"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDQ7014646.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1565..3358
                     /gene="hyaB"
                     /locus_tag="QBH27_002344"
     CDS             1565..3358
                     /gene="hyaB"
                     /locus_tag="QBH27_002344"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDQ7014647.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQLIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3377..4084
                     /gene="hyaC"
                     /locus_tag="QBH27_002345"
     CDS             3377..4084
                     /gene="hyaC"
                     /locus_tag="QBH27_002345"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDQ7014648.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4081..4668
                     /locus_tag="QBH27_002346"
     CDS             4081..4668
                     /locus_tag="QBH27_002346"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDQ7014649.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGIRLRQYRMTLEEQG"
     gene            4665..5063
                     /gene="hyaE"
                     /locus_tag="QBH27_002347"
     CDS             4665..5063
                     /gene="hyaE"
                     /locus_tag="QBH27_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDQ7014650.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTCGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5060..5917
                     /locus_tag="QBH27_002348"
     CDS             5060..5917
                     /locus_tag="QBH27_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDQ7014651.1"
                     /translation="MSETFFHLLGPGTQLNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            6051..7595
                     /gene="appC"
                     /locus_tag="QBH27_002349"
     CDS             6051..7595
                     /gene="appC"
                     /locus_tag="QBH27_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDQ7014652.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            7607..8743
                     /gene="appB"
                     /locus_tag="QBH27_002350"
     CDS             7607..8743
                     /gene="appB"
                     /locus_tag="QBH27_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDQ7014653.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            8756..8848
                     /gene="cbdX"
                     /locus_tag="QBH27_002351"
     CDS             8756..8848
                     /gene="cbdX"
                     /locus_tag="QBH27_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDQ7014654.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            8928..10238
                     /gene="appA"
                     /locus_tag="QBH27_002352"
     CDS             8928..10238
                     /gene="appA"
                     /locus_tag="QBH27_002352"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDQ7014655.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKEGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEVRIPACALHQDK"
     gene            complement(10226..10438)
                     /gene="cspH"
                     /locus_tag="QBH27_002353"
     CDS             complement(10226..10438)
                     /gene="cspH"
                     /locus_tag="QBH27_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDQ7014656.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            10724..10936
                     /gene="cspG"
                     /locus_tag="QBH27_002354"
     CDS             10724..10936
                     /gene="cspG"
                     /locus_tag="QBH27_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDQ7014657.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            10947..11135
                     /locus_tag="QBH27_002355"
     CDS             10947..11135
                     /locus_tag="QBH27_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDQ7014658.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            11110..11340
                     /gene="ymcE"
                     /locus_tag="QBH27_002356"
     CDS             11110..11340
                     /gene="ymcE"
                     /locus_tag="QBH27_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDQ7014659.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            11330..11503
                     /locus_tag="QBH27_002357"
     CDS             11330..11503
                     /locus_tag="QBH27_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDQ7014660.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(11552..12625)
                     /locus_tag="QBH27_002358"
     CDS             complement(11552..12625)
                     /locus_tag="QBH27_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDQ7014661.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLAFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(12708..15440)
                     /gene="torS"
                     /locus_tag="QBH27_002359"
     CDS             complement(12708..15440)
                     /gene="torS"
                     /locus_tag="QBH27_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDQ7014662.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRIDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRVPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            15523..16551
                     /gene="torT"
                     /locus_tag="QBH27_002360"
     CDS             15523..16551
                     /gene="torT"
                     /locus_tag="QBH27_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDQ7014663.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(16524..17216)
                     /gene="torR"
                     /locus_tag="QBH27_002361"
     CDS             complement(16524..17216)
                     /gene="torR"
                     /locus_tag="QBH27_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDQ7014664.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            17346..18518
                     /gene="torC"
                     /locus_tag="QBH27_002362"
     CDS             17346..18518
                     /gene="torC"
                     /locus_tag="QBH27_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDQ7014665.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            18518..21064
                     /gene="torA"
                     /locus_tag="QBH27_002363"
     CDS             18518..21064
                     /gene="torA"
                     /locus_tag="QBH27_002363"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDQ7014666.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVSTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            21061..21660
                     /gene="torD"
                     /locus_tag="QBH27_002364"
     CDS             21061..21660
                     /gene="torD"
                     /locus_tag="QBH27_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDQ7014667.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(21915..22220)
                     /gene="cbpM"
                     /locus_tag="QBH27_002365"
     CDS             complement(21915..22220)
                     /gene="cbpM"
                     /locus_tag="QBH27_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDQ7014668.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(22220..23140)
                     /gene="cbpA"
                     /locus_tag="QBH27_002366"
     CDS             complement(22220..23140)
                     /gene="cbpA"
                     /locus_tag="QBH27_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDQ7014669.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            23675..24916
                     /gene="agp"
                     /locus_tag="QBH27_002367"
     CDS             23675..24916
                     /gene="agp"
                     /locus_tag="QBH27_002367"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDQ7014670.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTERTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
BASE COUNT         5876 a         6370 c         6761 g         5967 t
ORIGIN      
        1 tcaaccgctc ggccacactt ccggaatgac gcgcactata aacatcccga tgcggcgtgt
       61 aaacccctaa tttgtttgtt tgcctgaaaa atagccaaaa gtgcattgat agcgtgaaac
      121 aacagcagat tgatcatttc atcaccatga attccttctc tatttactcg tttagcaacc
      181 ggctaaacat ccccaccacc cggcaaaaag aaaaataggt ccatttttat cgctaaaaga
      241 taaatccaca cagtttgtat tgttttgtgc aaaagtttca ctacgcttta ttaacaatac
      301 tttctggcga cgtgcgccag tgcagaagga tgagctttcg ttttcagcat ctcacgtgaa
      361 gcgatggttt gccttgctac agggacgtcg cttgccgacc atgtgcgccc ggtgttctgc
      421 cggtgtcgca aggagaagag acgtgcgata tgaataacga ggaaacattt taccaggcca
      481 tgcggcgtca gggcgttacc cggcgcagct ttctcaaata ttgtagtctg gctgccacgt
      541 cgctgggatt aggcgcggga atggcaccaa agattgcctg ggcgctggag aataaaccgc
      601 gcattccggt ggtatggatc cacggcctgg aatgcacctg ctgtaccgaa tcttttatcc
      661 gctccgctca cccactggcg aaggacgtca tcctttccct gatttccctc gattacgacg
      721 atactttgat ggctgccgcc ggaacccagg cggaagaagt ctttgaagac atcatcgcgc
      781 aatacaatgg caaatatatc ctcgcagtag aaggtaatcc gccgctgggc gagcagggga
      841 tgttctgtat cagcagcgga cgaccgttta ttgagaaact caagcgtgcc gccgctggag
      901 ccagtgcgat tatcgcctgg ggaacctgcg cgtcctgggg ctgcgttcag gccgcgcgac
      961 ctaatccgac gcaggcaacg cctatcgaca aagtcatcac cgacaaaccc attatcaaag
     1021 tacctggctg cccgccgatc ccggatgtga tgagcgccat cattacttac atggtgacgt
     1081 ttgatcgtct gccggatgtc gacagaatgg gccgtccact gatgttttat ggtcagcgta
     1141 tccacgataa atgctatcgc cgcgctcact tcgacgccgg agagttcgtc cagagttggg
     1201 atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat gggctgcaaa gggcctacca
     1261 cctataacgc ctgttcctcc acacgctgga acgatggcgt ttccttcccg atccagtcag
     1321 gtcacggctg cctgggttgt gcggaaaatg gtttctggga tcgcggttcg ttctacagcc
     1381 gcgtggtcga tattccacaa atgggtaccc attccaccgc cgacaccgtc ggtttaaccg
     1441 cgcttggcgt ggtggcagcg gctgttggtg tgcacgcagt cgccagcgcc gttgaccagc
     1501 gcagacgtca taaccagcaa cctacagaaa ccgaacatca gccaggcaat gaggataaac
     1561 aggcatgagc actcagtacg aaactcaggg atacaccatc aacaatgccg gacgccgcct
     1621 ggtggtcgac ccgattacgc gcattgaagg ccacatgcgc tgcgaagtga atattaacga
     1681 tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg tttcgcgggc tggagatcat
     1741 tctgcaaggg cgtgacccgc gcgatgcgtg ggcgttcgtt gaacgtatct gcggcgtctg
     1801 tactggcgta cacgccctgg cttcggttta cgccatcgaa gatgccatcg gtattaaagt
     1861 gccggacaac gccaatatca tccgcaacat tatgctggca acgctctggt gccacgatca
     1921 tctggtgcac ttctatcagc ttgccgggat ggattggatc gatgtgttag atgcgctgaa
     1981 agccgacccg cggaaaacct ccgaactggc gcaaagcctc tcctcatggc cgaaatcatc
     2041 ccctggctat ttcttcgacg tacaaaaccg cctgaagaaa ttcgttgaag gcggccagtt
     2101 ggggatcttc cgcaatggct actgggggca cccgcagtac aaactgccgc cagaagccaa
     2161 cctgatgggc tttgcccact atctcgaagc tctcgatttc cagcgtgaaa ttgtcaaaat
     2221 ccacgcggtc tttggcggta aaaacccgca tccaaactgg attgtcggtg ggatgccttg
     2281 cgccattaac attgacgaaa gcggcgcggt cggggcagtc aatatggaac gcctgaacct
     2341 ggtgcagttg attatcaccc gcacggcgga tttcattaac aacgtgatga tccccgacgc
     2401 cttagccatc ggtcagttca acaaaccgtg gagcgaaatc ggcacggggc tttctgataa
     2461 atgcgtcctc agttacggcg cgttccctga tatcgccaac gactttggcg agaaaagtct
     2521 gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac aatgtactgc cagtggattt
     2581 agttgatccg cagcaggtgc aggagtttgt tgaccatgcc tggtatcgtt atcccaacga
     2641 tcaggttggg cgtcatccgt tcgatggcat taccgacccg tggtacaacc ccggcgatgt
     2701 caaaggcagc gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggatcaa
     2761 agcgccacgc tggcgcggta acgcgatgga agtggggccg ctggcacgca cgttaatcgc
     2821 ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat cgtatgatgt cggcgttgaa
     2881 actgccgctt tccggcatcc agtcaacgtt gggccgcatt ttgtgtcgcg cgcacgaagc
     2941 gcagtgggcc gcaggtaagt tgcagtattt cttcgataaa ctgatgacca acctgaaaaa
     3001 cggcaatctt gccaccgctt ccacggaaaa atgggaacca gcaacctggc cgacagagtg
     3061 ccgtggtgtc ggttttaccg aagcgccgcg cggggcgtta ggccactggg ctgccattcg
     3121 cgatggcaag attgatctct atcagtgcgt ggtgccgacc acctggaacg ccagcccgcg
     3181 cgatcccaaa gggcagattg gcgcttatga agcggcgctg atgaacacca aaatggcgat
     3241 ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc tttgacccgt gcctcgcctg
     3301 ttcaacacac gtgctgggcg acgacggtag cgagcttatc tccgtgcagg tgcgttaaca
     3361 gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt gtcagtcact atgtctttga
     3421 agcacccgtg cggatatggc actggctgac agtgttatgc atggcggtgt tgatggtcac
     3481 cggttacttt atcggcaagc cgctaccttc cgtcagcggc gaggcgacat atctgttcta
     3541 tatgggctac atcaggttaa ttcacttcag cgccgggatg atttttaccg tggttttgct
     3601 gatgcggatc tactgggctt ttgttggcaa tcgttactcc cgcgagctgt ttatcgtgcc
     3661 ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa atccgctggt atctgtttct
     3721 ggcaaaacgt ccgagtgccg atataggcca taaccccatc gcccaggcgg cgatgttcgg
     3781 ctatttcctg atgtcagttt ttatgatcat caccggcttt gcgctgtaca gcgaacacag
     3841 ccagtacgct atttttgcgc cgttccgtta tgtggtggaa tttttctact ggacgggcgg
     3901 caactcaatg gacattcaca gctggcatcg gctggggatg tggctgattg gcgcatttgt
     3961 gatcggccat gtctacatgg cgctgcgtga agacatcatg tccgacgaca cggtgatctc
     4021 cactatggtc aacggctacc gtagccacaa atttggcaaa ataagtaaca aggagcgttc
     4081 atgagcgagc aacgcgtggt ggtaatgggg ctgggcaacc tgctgtgggc cgatgaaggc
     4141 ttcggcgtgc gggtggcgga acggctgtat gcccattacc actggcccga ggatgtggag
     4201 attgtcgatg gcggcaccca aggactgaac ttgctggggt atgtcgaaag cgccagtcat
     4261 ctgttgattc tcgatgccat tgattacggg ctggaacccg gaacgctacg aacgtatgcc
     4321 ggagaacgca ttccggctta tctcagcgcg aagaaaatga gcctgcacca gaacagtttc
     4381 tccgaagtgc tggcgctggc agatatccgc ggacatctgc cagcacatat tgccctcgtc
     4441 ggtctgcaac ccgcaatgct cgacgactac ggcggcagcc tgagcgaact ggcgcgggag
     4501 caactgcccg ccgcggaaca ggcggcgctg gcgcagctgg ctgcgtgggg aattgtgccg
     4561 caaccggcta atgaatcgcg ctgtctcaat tatgactgtc tgtcgatgga aaattacgaa
     4621 ggcattcgtt tgcgccagta ccggatgaca ctggaggagc agggatgagc aacgacacgc
     4681 catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg gacgccgatc agtgaatccc
     4741 gtcttgacga ctggcttacg caagcgccag acggcgtggt gttattaagc agcgatccga
     4801 aacgcacgcc agaggtcagc gataatccgg taatgattgg cgaattattg cgcgagtttc
     4861 ccgattatac atggcaggta gcgattgctg accttgagca gagcgaagcc atcggcgatc
     4921 gctttggcgt ctttcgcttt cccgccacat tagtatttac ctgcggaaac tatcgcggcg
     4981 tgctgaatgg tattcacccg tgggcggaac tgataaacct gatgcgcgga cttgtcgaac
     5041 cgcagcagga gcgtgcctca tgagcgaaac ttttttccat ctgctggggc caggaacgca
     5101 actgaacgat gacagtttca gcatgaatcc actgccgatc acctgtcagg tgaatgatga
     5161 accgagtatg gcggccctgg agcaatgtgc tcacagcccg caggtgattg cgctgttaaa
     5221 cgagttacaa catcaactaa gcgaacgcca gccgccgttg ggcgaggtac tggcagtcga
     5281 tctgttaaat ctcaacgccg acgatcgtca ctttatcaat acgcttctcg gggaagggga
     5341 agtgtcagtg cgcattcagc aggctgatgg tagtgaaagt gaaattcagg aggcgatctt
     5401 ctgcggatta tggcgggtac gcagacgtca cggcgaacag ttgctggaag acaaactgga
     5461 ggctggctgc gcaccgctgg cgttgtggca ggcggcaacg caaaacgtct tgccgacaga
     5521 ttcgctgtta ccgccgccca ttgatggcct gatgaatggc ctaccgttgg cccatgaatt
     5581 actggcacat gtacgtaacc ccgacgcaca gccgcacagc attaatctga cgcaattacc
     5641 catcagcgaa gctgaccggc tttttctctc acgtctctgt gggccgggaa atattcagat
     5701 tcgtaccatt ggctatggcg agagctatat caacgccacg gggttacgcc atgtctggca
     5761 tttacgctgt acggacacct taaaaggccc gttactggaa agttatgaaa tctgcccaat
     5821 accggaagtg gtgctggcag cgccagaaga tttggtcgac tctgcgcagc ggcttagcga
     5881 ggtatgccag tggctggcgg aaggtgcacc gacgtaaaaa gacggtaagt atcgctttca
     5941 ggcttatgaa tatcgcaatc ggcgaatacc tctggtcgta gagtttcagg ataaagaggg
     6001 agatctacca ttaacgggtt atttttctct cttcgcctac aggagtgcgc atgtgggatg
     6061 tcattgattt atcgcgctgg cagtttgctc tgaccgcgct gtatcacttt ttatttgtac
     6121 cccttaccct ggggctgatt tttttgctgg ctatcatgga aaccatttac gtggtcaccg
     6181 gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa gctcttcggt atcaactttg
     6241 ctcttggcgt ggctaccggc ctgacgatgg agtttcagtt tggtactaac tggtcattct
     6301 attccaacta tgtgggcgat atttttggcg caccactggc gatggaagca ttaatggcct
     6361 tcttcctcga atccaccttt gtcgggctgt tcttcttcgg ctggcaacgg ctgaataaat
     6421 accagcacct gctggtgacg tggctggtgg cgttcggttc aaatctctct gcgttgtgga
     6481 tattgaatgc caacggttgg atgcaatacc cgactggtgc gcattttgat atcgacaccc
     6541 tgcgtatgga gatgaccagt ttcagcgagc tggtctttaa cccggtcagc caggtgaaat
     6601 ttgtgcacac cgtaatggcg ggatacgtga ccggggctat gtttattatg gcgatcagcg
     6661 cctggtattt actgcgcgga cgggagcgcg atgtcgcatt acgctcgttt gccatcggtt
     6721 ccgtcttcgg tactctggcg attatcggta cgctgcaact cggagacagt tctgcgtatg
     6781 aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga aggggagtgg caaacagaac
     6841 ctgcacctgc accgttccat gtggttgcct ggccggaaca ggatcaagag cgtaacgcct
     6901 ttgccatcaa aattcccgcg ctgctaggga tcctcgccac tcactcatta gataaacccg
     6961 tgccgggtct gaagaatttg atggctgaaa cctaccctcg cttgcaacgt ggacgtatgg
     7021 cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga gccgcatgtg ttgcaggcat
     7081 tccgggagct ggaaggcgac ctgggttacg gcatgttgct ctcccgctat gcgccggata
     7141 tgaatcatgt cacagccgca cagtaccagg cggcgatgcg tggcgcgata cctcaggttg
     7201 cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg ttccctgctg ctactggtga
     7261 tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga ccagcatcgc tgggtgctga
     7321 aaatggcgct ctggagtttg ccgctgccgt ggattgcgat tgaagccgga tggtttatga
     7381 ccgagtttgg tcgtcagccg tgggcgatac aggacatctt accgacatac tccgcgcact
     7441 ccgccttaac cacaggacaa ctggctttct cactgatcat gatcgtaggg ctttacaccc
     7501 tgttcttaat cgccgaagtc tacctgatgc agaaatatgc ccgtctgggg ccgagcgcga
     7561 tgcagagtga acaaccgacg cagcaacagg ggtaaaggag aaaatcatgt ttgattatga
     7621 aacattgcgt ttcatctggt ggctgctgat tggcgtgatc ctggtggtct ttatgatctc
     7681 cgacggattt gacatgggga tcggctgtct gctgccgctg gtggcgcgta atgatgatga
     7741 acgtcggata gtgataaaca gcgttggcgc gcactgggaa ggcaatcagg tctggttgat
     7801 cctcgcaggt ggggcattat ttgccgcctg gcccagagtg tatgcagcgg cgttttccgg
     7861 cttttatgtg gcgatgattc tggtgctgtg ctcgctgttc ttccgcccgc tggcctttga
     7921 ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg tgggatgccg gactggtcat
     7981 cggcagtctg gtgccgccgg tagtcttcgg tattgcattc ggcaacttgc tgctcggcgt
     8041 gccgtttgct ttcacaccgc aattacgcgt ggaatatctc ggcagcttct ggcaactgct
     8101 gacgccattc cctttattgt gtggattgct cagtcttggg atggtgattt tgcaaggtgg
     8161 cgtctggtta caactgaaaa ctgttggtgt gattcatctg cgttcacagc tggcgaccaa
     8221 acgcgccgca ctactggtga tgctgtgctt tttgctggcg ggttactggc tgtgggttgg
     8281 tattgatggc tttgtgctgc tcgcccagga tgctaacggt ccttccaatc cattaatgaa
     8341 actggtggca gtgctacctg gtgcctggat gaataatttc gtcgaatcgc ccgttttgtg
     8401 gatcttcccg ctgctggggt tcttctgccc attgctgacg gtgatggcga tttatcgtgg
     8461 tcgcccgggt tggggatttt tgatggcgtc attgatgcaa tttggcgtga ttttcacggc
     8521 aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg agtccgatct ccagcctgac
     8581 gttgtgggac agcacttcca gtcagctgac gctgagcatt atgttggtaa tcgtgctgat
     8641 atttttgccc attgtgttgc tctacactct ctggagctac tacaaaatgt gggggcgcat
     8701 gacgacagaa actctccgcc gtaacgaaaa cgagctgtac taaggagcag gaacaatgtg
     8761 gtatttactt tggttcgtcg gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt
     8821 atggctggac ccgcgtctga aaagttaacg aaggtaagcc tgatgaggcg cattagcgtc
     8881 gcatcaggca atcaataatg tcggatatga aaagcggaaa catatcgatg aaagcgatct
     8941 tgatcccatt cttatctctt ctgattccgt taacaccgaa atctgcattc gctcagagtg
     9001 agccggagct gaagctggaa agtgtggtga ttgtcagtcg tcacggtgtg cgtgcaccaa
     9061 ccaaggccac gcaactgatg caggatgtca cccctgacgc atggccaacc tggccggtaa
     9121 aactgggttg gctgacaccg cgcggtggtg agctaatcgc ttatctcgga cattaccaac
     9181 gccagcgtct ggtggccgac ggattgctgg caaaagaggg ctgcccgcag ccaggtcagg
     9241 tcgcgattat tgctgatgtc gacgaacgta cccgtaaaac aggcgaagcc ttcgccgccg
     9301 ggctggcacc tgactgtgca ataaccgtac atacccaggc agatacgtcc agtcccgatc
     9361 cgttatttaa tcctctaaaa actggcgttt gccaactgga taacgcgaac gtgactgacg
     9421 cgatcctcag tagggcagga gggtcaattg ctgactttac cgggcatcgg caaacggcgt
     9481 ttcgcgaact ggaacgggtg cttaatttcc cgcaatcaaa cttgtgcctt aaccgtgaga
     9541 aacaggacga aagctgttca ttaacgcagg cattaccatc ggaactcaag gtgagcgccg
     9601 acaatgtctc attaaccggt gcggtaagcc tcgcatcaat gctgacggag atatttctcc
     9661 tgcaacaagc acagggaatg ccggagccgg ggtggggaag gatcaccgat tcacaccagt
     9721 ggaacacctt gctaagtcta cataacgcgc agttttattt gctgcaacgc acaccagagg
     9781 ttgcccgcag tcgcgccacc ccgttattgg atttgatcat gacagcgttg gcgccccatc
     9841 caccgcaaaa acaggtgtat ggtgtgacat tacccacttc agtgctgttt atcgccggac
     9901 acgatactaa tctggcaaat ctcggcggcg cactggagct caactggacg cttccaggtc
     9961 agccggataa cacgccgcca gggggtgaac tggtgtttga acgctggcgt cggctcagcg
    10021 ataacagcca gtggattcag gtttcgctgg tcttccagac tttacagcag atgcgtgata
    10081 aaacgccgct gtcattaaat acgccgcccg gagaggtgaa actgaccctg gcaggatgtg
    10141 aagagcgaaa tgcgcagggc atgtgttcgt tggccggttt tacgcaaatc gtgaatgaag
    10201 tacgcatacc agcgtgcgct ctccatcaag acaaataaac atttgccgct gttggtccac
    10261 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    10321 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    10381 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    10441 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    10501 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgcgttaa
    10561 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    10621 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag tttctgtatt
    10681 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaaatgac
    10741 tggtttagta aaatggttta acgcagataa aggttttggc tttatcactc ctgatgatgg
    10801 cagcaaagac gttttcgtcc atttcactgc catccagagc aatgaattcc gcacgctgaa
    10861 cgaaaatcag aaagttgaat tttctattga gcaggggcaa cgtggtcccg cggcagcgaa
    10921 cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc tgtccatgtt
    10981 gccatggttc acagtaccgc acatcggcat tcgatgtgac ggagcgaaac cctttgggtg
    11041 caaagtgcat tttttgtaaa tcgacgatga tcacctttga taacgttgcg ctgcaaatac
    11101 gcactgacca tgcgccgctg gatttcacaa aataatatca ggctccctcg tggagccttt
    11161 tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa cctgttgatt
    11221 attgaatctt tcggggagat ggcttataac atttcttacc tgaccagggt accgggaacc
    11281 aacaccttac tggcgtgttg ctgtctttta agaccagaag aggttaacag tgaatattga
    11341 agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa aaatcgccga
    11401 gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcaccgcac gagaaaaaat
    11461 gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg aacatcaccc
    11521 cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc aatcattggc gattgcggaa
    11581 ttaagcgttg gtacaattcc tccggcaccg ggctttgcca tactcccgca tacattgcgt
    11641 aaccaatcgc cgcaaacata atccccataa ccagtagcgt cattaaccag ccagacaacg
    11701 caaaggcttt tttatttgcc gcaggttttt gcagtgaaaa ggccaatgtt gaggctaccg
    11761 gacatgactc tacgcaagtc atacagccgg tacattccac tgttcgtacc tgaattaatt
    11821 tatcgaccgg gatccgtgat gggcaacttt ttgcgcattt gccacaatcg atacaacttt
    11881 cggcattgcg acgaatctta aacggcgata atagcgaaac cacgcccatc agcgcgccat
    11941 atgggcaaag ataacgacac caggcatggc gaataaacag gctggcaatc agcaaaacga
    12001 tcatgctgat taatgtcgcg gtccccatat gacgaaagaa atcgagcatt ttaacgtcca
    12061 tcaccacgct gtagggcgac aacataaaat agtgaatcgc ctgagcgggc atcaataacg
    12121 cgatatagag aaaaaaactc aacagcaaat acttcacgcc gcgcagagga atatccagcc
    12181 agcggggaag gacaaattgc cgaccaaaca gtttgttacc gagatcgccg attaattccg
    12241 aaagcgtacc aaccgggcat aaccatgaac aaaaggcctt tttgagtaat agactgatga
    12301 cgataaaagc gaccaataac agcatcgcgg cggcgtggac ggacggtaac tgacctgttg
    12361 caaggctata tttcagattc atcagaccgg caatcggtag ccagccttcg ataccacccg
    12421 gtctggtgac aaatgtcgta ctacctgccg tttcgtaata gcgcacccaa taccagaacg
    12481 tgatggcaat ataaatatta attgccaaca gtaataattg cgtcgcttta cgccaggtcg
    12541 tggcattgcg ccagtcattc cacggtaatt tgccgcccgt cgtgcctggc cgccgttgcc
    12601 agcgggttct ttttttctct gccatgattt tgccagtccg ttaagttgca taccaaatgc
    12661 cactattcta gttgttctta actggctgat attgattcaa atcgcgttca ggtctttctt
    12721 attaaccatg cttccagagc ggcaacactg cgtgtaattt cttcgtgtgg aaggggggca
    12781 gataatggct gctgctccag ttgtgcgcat tgctggctgg cgctacgcat tcccagactc
    12841 gagcaactgc tttttagctg atgtgcggca cgctttattt tctcgttatc ctgactggcg
    12901 cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtttaaataa tatcagccat
    12961 tcgtggatct tctccgcccc cattaaatga gcatcttcat tgagttgcga tacatccagt
    13021 ggttgatcgt tattgacttg cagttggaga tagtgcgcca gtaattgacc gagcacttca
    13081 cgcggcaccg gtttagggat aatcccgcga aatagcgaac tggtacgctg tcgcagtgtt
    13141 tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg ctgtgccagt
    13201 tgtcgggcaa gcgtaatgcc gtcgatatcc ggtagatcaa aatccaccag tgcggcagca
    13261 aacggttcac tattttgcag tgtctctaaa gcctgcgcgg catttcctac ggcaacgacc
    13321 tgcgccccac tgctgttcaa catctcaacg gtaatccgct gggttagcgg gttatcttca
    13381 attaacagca aacgtaaacc gtccagacgc accgcctgat tgactgtttt tggcacgggt
    13441 gcagtggcaa cacgtaacgg cacgcgtaaa caaaaacagc ttccaacctc cggcgtgctg
    13501 gtggcgctca attcgccgcc catcgcctgg gccagacggc tactgatagt cagccccagc
    13561 ccggtgccgc cgcgtttgcc gctaacctga acaaatggct ggaagatttc agccagtttc
    13621 gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgttc gccatcaatg
    13681 cgactacgca gaataatgtg cccttcgtcg gtgaaacgca gggcgttgct caacaggttg
    13741 gtaatgacct gacgaatacg ccgcggatcg cccattaacg cagtcggtac atcgtcggca
    13801 atttctgttg ccaggcgaat cgggcgacct ttcacccttc cgctcattaa ttgcagggta
    13861 ctttccagca gcgggcgcgg ttcaaagggt tcatcgctga ccgaaacatt cttgccgcct
    13921 gcttcgatag ccgaataatc gagaatatcg ttgaggatgg tcagcaacga ttcaccagag
    13981 tcggtaatcg cccgcaaatc atcgcgctgg gcgttaagtg cggggttatc tgccagcagc
    14041 tgtgcggtgc cgagaatacc gtacagcggt gtgcggatct catggctcat cgccgccaga
    14101 aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg gtgttctatc
    14161 accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg ttcgcggtgg
    14221 cgattcagtg catgaacact gctgcgaaac gcatccatca gccgcccgat ggtatccagt
    14281 tcccgtacgc cagcggtttc cgggaaaggg gagtcgatat caccatccag caagcgttgc
    14341 agcgcctgcg tttgttctgc aagagggcgc gtgactgagc gataaaccac gcgccagagg
    14401 atcagaatca gtgcgcaaag agaaactaat cccagcaata acaggctgta ttgcccgcgt
    14461 gcactggctt tttccagatg agccagcccg tgctgattac gcagctcaat ggtgtcgacc
    14521 agctgactga cttcgctact aaactgcgcg aattgggcga tgttattttg ggcgagagtt
    14581 tgcaggtgat tgctgatttc actgtcctgc tgatacagcg ccagcaaatc gctatattgg
    14641 ctaacggtag ttaacgttgt tgctacctga gtacgaacac ccggatcttc aatgcgtatt
    14701 tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt tggcgcattt
    14761 ttctggatct gttccagccc cagattcatc accatttgct gcacccgcag ggcgctaagg
    14821 cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg atcgagtgca
    14881 ctttctgcgg cttgacgctg atgttgttcg atcaaatcgt aaatcccggc ctgggtcgct
    14941 ccggcggagg ttgccgcatt attcgcctga ccttgcgcca ggcgtgcgat ttcatcggcg
    15001 gcggcgacta tctgctgact gagttgctgt tgttgctggc gaagttgcaa acgctgcccc
    15061 accagttccc cttgctgacg taacgaacgg gagatctcct gctcctgttg ttcaatagcg
    15121 gtggtgtcaa aaccttgttc ccgtaacgct tgcagcaacg cattaatctt cagactttgt
    15181 gcggtgagca ttcgcccctg cgcctgccac atcttttcgt tatcggcact ggtcaggttc
    15241 tgcgcggcga aaagttccca ggcgctggct tcgctcaact ggcgcgccat attcatggta
    15301 ggaatcaatg cctgagtgtt gtctttttcc acctggctga taaagcgcag gttgtaccat
    15361 cccaccaggg tactggtcag ggttaacagc gccatcaggg caaagcccat ccagagtctt
    15421 cgggtcaggg ttaaattcac ggtcggtgca cttaaggtga aaaaggttga gtcgcaaagc
    15481 ggaatgcatc tagcataaag ccttattatt gatgaggcta tcatgcgcgt actgctattt
    15541 ttacttcttt cccttttcat gttatcggca ttttcggctg ataacctgtt gcgctggcat
    15601 gatgcgcagc atttcacggt gcaagcctct acgccgctta aagccaaacg cgcatggaaa
    15661 ctgtgcgcgc tttatcccag cctgaaagat tcatactggt tatcgttgaa ctatggtatg
    15721 caggaggctg ctcgccgcta cggtgtggat ttaaaagtgc tggaggcagg cggctacagc
    15781 cagttggcta cccagcaagc acaaatcgac cagtgtaaac agtggggcgc agaggccatt
    15841 ttgctcggta gtagcacgac gtcatttccc gatctgcaaa agcaggtagc aaatctgccg
    15901 gtgatcgaac tggtaaatgc tattgatgct ccccacgtga aaagccgcgt tggtgtgccc
    15961 tggtttcaga tgggctatca accagggcga tatctggtgc aatgggcgca cggtaaacca
    16021 ctgaacgtgc tgttgatgcc cggccccgat aacgccgggg gcagtaagga gatggtagag
    16081 ggttttcgcg cagccattgc cggaagcccg gtgcgtattg ttgatattgc gctcggtgat
    16141 aacgatattg aaatccagcg taacctgttg caggagatgc tggaacgcca tccagaaatc
    16201 gacgtcgttg ccggaacggc cattgcggca gaggcggcaa tgggggaagg gcgtaacctg
    16261 aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc aggtgtatcg cgggttgaag
    16321 cggggaagag tgattatggc tgccagcgat caaatggtct ggcaagggga actggcggtt
    16381 gagcaggcca tcaggcaatt acaggggcaa tcggtgtctg ataatgtcag cccaccgatt
    16441 ttagtcatga cgccgaaaaa tgccgaccgc gaacatatcc gccgctcgct gtcaccaggg
    16501 ggatttcgtc cggtttattt ttatcagcac acatcagcgg ctaagaaata accttcgcca
    16561 tgttgtgtca ccagtaaatc cgcactgagt ttatggcgta aacgacgaat taacacatcg
    16621 acggtgcgca ggtcagggtt ttccacccga cgcgcagaga gcatacgcag cagacgttca
    16681 cggctgagaa tttcgcccgg attagtcaca aatgccacca gcatttcata ctctgcgcgg
    16741 gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc gcgacacatt caggcaataa
    16801 ccggcaaagc gatagcagtt gtcctgagtg tgcggttgcg cctgtcgcgc gaggtcgatt
    16861 cgccagagca aatttttcac tcgtaccacc agttcgcgca gctccagcgg tttggtgacg
    16921 taatcgtctg cgcccatttc cagcccaaca atacggtcaa tccgatcgct gcgtccggta
    16981 accagaataa tccctaccgt tgagcgttct cgcagggcgc gggttaacat caggccattt
    17041 tcatcgggca aattgatatc cagcagaatc aaatccaccg gctgattctg cataatttcc
    17101 cgcagcccgg caccgctcgc cgtaacggaa acggtatacc cctcctgagt gaagtaagat
    17161 tgtaatcgcg cctgggtaac cggctcatct tcaacaataa caatgtgatg tggcatcaga
    17221 gggttttact cattctgttc atatctgttc atattctgcc gtaagccgtt catcctgacc
    17281 agtgccgctg ttcatatttg ctcattaaga tcgcttcact aaaccataat tttacagggg
    17341 ctattatgcg gaaactctgg aacgcgctac gccgacccag tgctcgttgg tcggtactgg
    17401 cgctggtcgc tactgggatt gtgattggca ttgcgctgat tgtattgcca cacgttggga
    17461 tcaaagtcac cagcacaacc gaattttgtg tcagttgcca cagtatgcaa ccggtgtatg
    17521 aagaatataa acagtcggtg catttccaga acgcctccgg cgtgcgagct gaatgccatg
    17581 actgtcatat cccgccggat attccaggca tggtgaagcg caaactggaa gcgagcaacg
    17641 acatttacca gacctttatt gcccactcca ttgatacacc tgagaaattt gaagccaaac
    17701 gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga aaacaactcg gcaacctgcc
    17761 gctcctgcca taactacgat gcgatggatc atgcgaagca gcatcctgaa gcggcgcgtc
    17821 agatgaaggt ggcagcgaaa gataatcaat cctgcatcga ctgtcataaa ggtattgccc
    17881 accagttacc ggatatgagt agtggcttcc gtaagcagtt cgatgagctg cgcgccagtg
    17941 ctaatgatag tggtgacacg ctgtactcca ttgatattaa gccgatttat gcggcgaaag
    18001 gcgataaaga agcgtctggt tctctgctgc ctgcttcgga agtgaaagtc cttaaacgtg
    18061 acggcgactg gctgcaaatt gaaatcactg gctggacgga aagtgcagga cgtcagcgtg
    18121 tactcaccca attcccaggt aaacgcatct ttgttgcctc gattcgtggt gatgtgcagc
    18181 agcaggtgaa aacactggag aaaaccaccg ttgccgacac caataccgag tggagcaagt
    18241 tgcaggccac tgcgtggatg aagaaaggcg acatggtgaa cgatatcaaa ccgatctggg
    18301 cttatgcgga ttcgctgtac aacggcacct gtaaccagtg ccacggcgca ccggaaatat
    18361 cccactttga cgctaacggt tggatcggca cgctcaacgg catgattggc tttaccagcc
    18421 tcgataaacg tgaagaacgc actttgttga aatatctgca aatgaatgcg tctgacaccg
    18481 caggtaaagc tcacggcgat aagaaggaag aaaaataatg aacaataacg atctctttca
    18541 ggcatcacgt cggcgttttc tggcacaact cggcggctta accgtcgccg ggatgctagg
    18601 gccgtcattg ttaacgccgc gccgtgcgac tgcggcgcaa gcggcgactg aggctgtcat
    18661 ctcgaaagag ggcattctta ccgggtcgca ctggggggct atccgcgcga cggtgaagga
    18721 tggtcgcttt gtggcagcaa aaccgttcga actggataaa tatccgtcga aaatgatcgc
    18781 cggattgccg gatcatgtac acaacgcggc gcgtattcgt tacccgatgg tacgcgtgga
    18841 ctggctgcgt aagcgccatc tgagcgacac ctcccagcgc ggtgataacc gttttgtgcg
    18901 cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa ctggaacgcg tacagaaaac
    18961 tcacgggccg agtgccttgc tgaccgccag tggttggcaa tcgacgggga tgttccataa
    19021 cgcttcgggg atgctggcga aagctattgc cttgcatggt aatagcgtta gtacgggcgg
    19081 agattactct accggtgctg cgcaggtgat cctgccgcgc gtggttggct caatggaagt
    19141 gtatgaacag caaacctcct ggccgctggt attgcagaac agcaaaacca ttgtgctgtg
    19201 gggctccgat ttgctgaaaa accagcaagc gaactggtgg tgcccggatc acgatgttta
    19261 tgaatattac gagcagctaa aagcgaaagt cgccgccggt gaaattgagg tcatcagcat
    19321 cgatccggtt gtcacatcca cccatgagta tctggggcgc gagcatgtga agcacattgc
    19381 ggttaacccg caaactgacg tgccgctgca actggcgctg gcgcatacgc tgtacagtga
    19441 aaacctgtac gacaaaaact tcctcgctaa ctactgtgta ggttttgagc agttcctgcc
    19501 gtatctgctg ggtgagaaag acggtcagcc gaaagatgcc gcatgggctg aaaaactgac
    19561 cggcattgat gccgaaacca ttcgtgggct ggcacggcag atggcggcga acagaacgca
    19621 gattattgct ggctggtgcg tacagcgtat gcagcacggt gaacagtggg cgtggatgat
    19681 tgtcgttctg gcggcgatgc tggggcaaat tggcctgcca ggtggtggtt ttggttttgg
    19741 ctggcactat aacggcgcag gcacgccggg gcgtaaaggc gttattttga gtggtttctc
    19801 cggctctacg tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac
    19861 cattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa
    19921 cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccgtt
    19981 ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat
    20041 cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac
    20101 cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaatc gtggcatcat
    20161 cgccatgaaa caggtcgtgc cgccgcagtt cgaggcgcgc aacgacttcg atattttccg
    20221 cgagctgtgc cgtcgcttta atcgcgaaga agcctttacc gaagggctgg acgaaatggg
    20281 ctggctgaaa cgcatctggc aggaaggtgt acaacagggc aaaggacgcg gcgttcatct
    20341 gccagcgttt gatgacttct ggaataacaa agagtatgtc gagtttgacc atccgcagat
    20401 gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag
    20461 tggcctgatt gagatctact cgaaaaccat cgctgatatg aactacgacg attgtcaggg
    20521 gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcctggct cgcagaagta
    20581 tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagt tatgtgagtc
    20641 ggaaactctg cgtcagcaat ataccgtagc gggtaaagag ccagtattta ttagcccgca
    20701 ggatgccagc gcgcgcggta ttcgtcacgg tgatgtggta cgcgtcttta acgttcgtgg
    20761 tcaggtgttg gctggagcag tggtttctga ccgttatgca cccggcgtgg cgcgaattca
    20821 cgaaggggca tggtacgatc cggacaaagg cggcgagcca ggggcgctgt gcaaatacgg
    20881 taaccccaac gtcttgacca tcgacatcgg tacttcgcag ctggcgcagg cgaccagtgc
    20941 gcacactacg ctggtggaaa ttgagaagta caacggaaca gtggagcagg tgacggcgtt
    21001 taacggcccc gtggagatgg tggcgcagtg cgagtatgtt cccgcgtcgc aggtgaaatc
    21061 atgaccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc
    21121 tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg
    21181 ttttcgttgc tgaaaagcga accgccgctc actgcggcgg tgaatgggct ggaaaacagt
    21241 attgccactc tgacagtacg tgacgatgcc cgtctggaac tggccgctga cttttgcggc
    21301 ctgtttctga tgaccgacaa acaagcggcg ctgccgtatg catcggccta caaacaggac
    21361 gagcaagaga ttaaacgctt gttagttgag gcggggatgg aaaccagcgg caatttcaac
    21421 gaaccggcag atcatctggc gatctatctc gatttgttga gtcatctgca tttttcgctg
    21481 ggagagggga ccgttcctgc gcgaagaatc gacggtttgc ggcaaaaaac actgacggct
    21541 ctgcgggaat ggttaccaga gtttgctgcg cgttgtcgac agtatgacag ctttggtttt
    21601 tacgcggcac taagccagtt attgctggtg ttagtggagt gtgactacca aaaaagataa
    21661 tgccgtttgt aggcctgata agacgcggca ggcgtcgcat caggcattag tgcgcagttg
    21721 ccggatgcgg cgagaacgcc ttatccggcc tacgggaaca gtgctttttg tgggcctgat
    21781 aagatgcgtt aagcgtcgca tcaggcatta tggcgcagtt gccggatgcg gcatgaacgc
    21841 cttatccggc ctacgggaac agtgctcttt gtaggcctga taagacgcgt taagcgtcgc
    21901 atcaggcaac aaactcacgg gtgagccaca aaccgggaaa gccgctggcg cagcaggcgg
    21961 ttttcctgct tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc
    22021 cagtccagag ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg
    22081 tcgtcaaata cccaggttgt ctcctggatc tcactcggtt caaccacccc caaaccgaca
    22141 atttcattca actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa
    22201 gtcaccgtaa cattagccat tatgctttcc cccaatcttt acgtggatca aaagacgact
    22261 gggcgtctgc cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca
    22321 cgattttcag taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt
    22381 taacgcgcaa tcgttgcccg gcctggctgc caggcgggat agtcagcaaa atgctttctt
    22441 tcagtgttgg aacggtgact ttagtaccca gcgccgcttc ccacgggcta accggcacca
    22501 ctatttccag atcctggccg acaatatcaa acagcggatg tggcgcaatg tgaatcacca
    22561 gccacaggtc gccatttgga ccgccgtttt cgcccggcgt cccctggcct ttcaggcgga
    22621 tgcgttgacc attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct
    22681 gttcgatcat gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct
    22741 cagtaagcgt ttcttcgagg aaaaccgcca cttcgatttc aatatcgtgg ccgcgtgtgg
    22801 cggggcgttg acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa
    22861 aatcttcggc gttaaaactc tgaccgtcgc catgatggaa ctgacggtta aattgcggat
    22921 cgttgcgatg ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt
    22981 cccaggcttc ggcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag
    23041 gatggtattt gcgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg
    23101 gtttcacgcc catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc
    23161 aacacaaatt gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa
    23221 tttcatatgc caacacagaa tatgttattg aaatcatcgc ggaggggagg ttgcctcaag
    23281 gtgggaggaa catattttat ttacctcact cctgaacttt tggctcatgc ttacaagtta
    23341 aacaataagg aaaggaatgc acaactcgac tatttaaatc gttatgcaat ttaatgataa
    23401 agacatgtgc taaaaaccat tgctattagt ctcacattct ttttttattg gtaatggtaa
    23461 cgccgctgat tttctttata gctactgatg taatcattag cggcgtaaac gaggccgggg
    23521 ctaatcaggc atagtttgcg tcaaaacttg cctgttttta aaggggaata tagaaaaaca
    23581 ggcgttcaac gagccatttt gcgaacctgt tcccggaaaa atgacatatt tctgtcacac
    23641 tctttaatta ttgataacaa aagaggtgtg aggaatgaac aaaacgctaa tcgccgcaac
    23701 tgtggcaggg atagttttac tcgcttcaaa tgctcaggca caaaccgtac cggaaggcta
    23761 tcagctacag caagtcctta tgatgagccg tcataactta cgtgcgccgc tggcgaacaa
    23821 tggcagtgtg ctggagcagt cgacgccgaa taaatggcct gaatgggacg tccccggtgg
    23881 gcagctcacc actaaagggg gcgtgctcga agtgtatatg ggccattata tgcgtgaatg
    23941 gctggcagaa caggggatgg tgaaatcagg ggaatgcccg ccaccggaca ccgtttatgc
    24001 ctatgccaat agtctgcaac gcaccgttgc gaccgcacag ttcttcatta ccggcgcatt
    24061 cccggggtgt gatattcctg ttcatcacca ggaaaaaatg ggcactatgg accccacctt
    24121 taacccagtg atcaccgatg attccgccgc attcagtgaa caggcggtgg cggcaatgga
    24181 gaaagagctc agcaaactcc agcttaccga cagctacaag ctactggaac aaatcgttaa
    24241 ctataaagat tctcctgcgt gtaaagagaa gcaacaatgt tcgcttgtag atggcaaaaa
    24301 tacctttagc gcgaagtatc aacaagaacc gggcgtttcc gggccgctga aagtcggcaa
    24361 ctcgctggta gatgcgttta ctttgcaata ctacgaaggt tttccgatgg atcaggtggc
    24421 ctggggagaa atcaaatctg accagcagtg gaaggtgttg tcgaagctga aaaacggtta
    24481 ccaggacagc ctgtttacct caccggaagt ggcgcgcaat gttgcgaaac cgctggtcag
    24541 ttatatcgac aaagccctgg tcactgaacg caccagtgca ccgaaaatca cggtattagt
    24601 tgggcacgac tccaacattg cctctctgtt aacggcgctg gatttcaaac cgtatcagtt
    24661 gcatgaccag aacgaacgca cgccgattgg cggcaaaatc gttttccagc gttggcatga
    24721 cagcaaagcc aatcgcgatt tgatgaaaat tgaatatgtg taccagagtg cggaacagtt
    24781 acgtaatgcc gatgcgttaa ccctgcaggc acctgcgcag cgcgtgacgc tggaattaag
    24841 cggttgcccg atagatgcca acggtttttg cccaatggat aagtttgatc gcgtattgaa
    24901 tgaagcggtg aaataacaga aaactccccc gcgtgaagcg ggggagtcgc tggttaaacg
    24961 tttttacgtt cgat
//
LOCUS       DAOFSV010000054        24953 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.054, whole genome shotgun sequence.
ACCESSION   DAOFSV010000054 DAOFSV010000000
VERSION     DAOFSV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.054"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            105..1148
                     /gene="pyrC"
                     /locus_tag="QBH27_002368"
     CDS             105..1148
                     /gene="pyrC"
                     /locus_tag="QBH27_002368"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDQ7014671.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVK"
     gene            1225..1470
                     /gene="dinI"
                     /locus_tag="QBH27_002369"
     CDS             1225..1470
                     /gene="dinI"
                     /locus_tag="QBH27_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDQ7014672.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            1759..2013
                     /gene="bssS"
                     /locus_tag="QBH27_002370"
     CDS             1759..2013
                     /gene="bssS"
                     /locus_tag="QBH27_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDQ7014673.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            2128..3246
                     /gene="solA"
                     /locus_tag="QBH27_002371"
     CDS             2128..3246
                     /gene="solA"
                     /locus_tag="QBH27_002371"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDQ7014674.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLHAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSADQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            3294..3407
                     /locus_tag="QBH27_002372"
     CDS             3294..3407
                     /locus_tag="QBH27_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDQ7014675.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            3668..4234
                     /locus_tag="QBH27_002373"
     CDS             3668..4234
                     /locus_tag="QBH27_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDQ7014676.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            4238..4813
                     /locus_tag="QBH27_002374"
     CDS             4238..4813
                     /locus_tag="QBH27_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDQ7014677.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(4855..5907)
                     /locus_tag="QBH27_002375"
     CDS             complement(4855..5907)
                     /locus_tag="QBH27_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDQ7014678.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            6132..7052
                     /gene="lpxL"
                     /locus_tag="QBH27_002376"
     CDS             6132..7052
                     /gene="lpxL"
                     /locus_tag="QBH27_002376"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDQ7014679.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            7224..8450
                     /gene="mdtG"
                     /locus_tag="QBH27_002377"
     CDS             7224..8450
                     /gene="mdtG"
                     /locus_tag="QBH27_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDQ7014680.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            8533..8907
                     /locus_tag="QBH27_002378"
     CDS             8533..8907
                     /locus_tag="QBH27_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HDQ7014681.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(8908..9135)
                     /locus_tag="QBH27_002379"
     CDS             complement(8908..9135)
                     /locus_tag="QBH27_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDQ7014682.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(9307..11850)
                     /gene="mdoH"
                     /locus_tag="QBH27_002380"
     CDS             complement(9307..11850)
                     /gene="mdoH"
                     /locus_tag="QBH27_002380"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDQ7014683.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(11843..13378)
                     /gene="mdoG"
                     /locus_tag="QBH27_002381"
     CDS             complement(11843..13378)
                     /gene="mdoG"
                     /locus_tag="QBH27_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDQ7014684.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            13772..14929
                     /gene="mdoC"
                     /locus_tag="QBH27_002382"
     CDS             13772..14929
                     /gene="mdoC"
                     /locus_tag="QBH27_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDQ7014685.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNLRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(14937..16418)
                     /gene="clsC"
                     /locus_tag="QBH27_002383"
     CDS             complement(14937..16418)
                     /gene="clsC"
                     /locus_tag="QBH27_002383"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDQ7014686.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADHIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTCLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(16360..16893)
                     /gene="ymdB"
                     /locus_tag="QBH27_002384"
     CDS             complement(16360..16893)
                     /gene="ymdB"
                     /locus_tag="QBH27_002384"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDQ7014687.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(16988..17299)
                     /locus_tag="QBH27_002385"
     CDS             complement(16988..17299)
                     /locus_tag="QBH27_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7014688.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNVQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(17420..17752)
                     /gene="csgC"
                     /locus_tag="QBH27_002386"
     CDS             complement(17420..17752)
                     /gene="csgC"
                     /locus_tag="QBH27_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDQ7014689.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(17812..18270)
                     /gene="csgA"
                     /locus_tag="QBH27_002387"
     CDS             complement(17812..18270)
                     /gene="csgA"
                     /locus_tag="QBH27_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDQ7014690.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(18311..18766)
                     /gene="csgB"
                     /locus_tag="QBH27_002388"
     CDS             complement(18311..18766)
                     /gene="csgB"
                     /locus_tag="QBH27_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDQ7014691.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            19519..20169
                     /gene="csgD"
                     /locus_tag="QBH27_002389"
     CDS             19519..20169
                     /gene="csgD"
                     /locus_tag="QBH27_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDQ7014692.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            20174..20563
                     /gene="csgE"
                     /locus_tag="QBH27_002390"
     CDS             20174..20563
                     /gene="csgE"
                     /locus_tag="QBH27_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDQ7014693.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            20588..21004
                     /gene="csgF"
                     /locus_tag="QBH27_002391"
     CDS             20588..21004
                     /gene="csgF"
                     /locus_tag="QBH27_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDQ7014694.1"
                     /translation="MRVKHAVVLLMLISPLSWAGSMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            21031..21864
                     /gene="csgG"
                     /locus_tag="QBH27_002392"
     CDS             21031..21864
                     /gene="csgG"
                     /locus_tag="QBH27_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDQ7014695.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKTERQNDILVKYRHMSVPPE
                     S"
     gene            complement(21929..22420)
                     /locus_tag="QBH27_002393"
     CDS             complement(21929..22420)
                     /locus_tag="QBH27_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDQ7014696.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(22522..23076)
                     /gene="ycdY"
                     /locus_tag="QBH27_002394"
     CDS             complement(22522..23076)
                     /gene="ycdY"
                     /locus_tag="QBH27_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDQ7014697.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(23100..23837)
                     /gene="ycdX"
                     /locus_tag="QBH27_002395"
     CDS             complement(23100..23837)
                     /gene="ycdX"
                     /locus_tag="QBH27_002395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDQ7014698.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKYQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(23892..24830)
                     /gene="ghrA"
                     /locus_tag="QBH27_002396"
     CDS             complement(23892..24830)
                     /gene="ghrA"
                     /locus_tag="QBH27_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDQ7014699.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRLQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         6412 a         6269 c         5819 g         6453 t
ORIGIN      
        1 cacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
       61 ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc
      121 aggttttaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
      181 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
      241 tggctccgcc cgtgaccacc gtagaggctg ccgtggcgta tcgccagcgt attcttgacg
      301 ccgtacctgc cgggcacgat ttcaccccgc tgatgacctg ttatttaaca gattcgctgg
      361 atcctaatga gctggagcgc ggatttaacg aaggcgtgtt caccgctgca aaactttatc
      421 cggcaaatgc aaccactaac tccagccacg gcgttacgtc agttgacgca atcatgccgg
      481 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtaacacatg
      541 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaaccgctgc
      601 gccagcgcct gaccgcgctg aaagtcgttt ttgagcatat caccaccaaa gacgctgccg
      661 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
      721 ttaaccgcaa ccatatgctg gttggtggtg tgcgtccgca cctgtattgt ctacccatcc
      781 tcaaacgcaa tatccaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgtg
      841 tattcctcgg aacggattct gcgccacatg cacgtcatcg caaagagagc agctgcggct
      901 gcgcgggctg tttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
      961 tgaatgcttt gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct
     1021 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
     1081 tcgcactgac tgatgacacg ctggtgccat tcctcgccgg agaaacggta cgctggtccg
     1141 ttaaataata aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac
     1201 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
     1261 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
     1321 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcaaataa tttatcggtt
     1381 attggcgcga caaaagaaga taaacagcgc attagcgaaa ttctccagga gacgtgggaa
     1441 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
     1501 ccccggcttt tttattttac ttgcgaattg tgagtacaaa atttctacac ttactcaaaa
     1561 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
     1621 ttaaaagcag aaatttatct gttccccctt taaaaattcg gtgaataccc ttacttattg
     1681 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
     1741 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
     1801 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
     1861 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
     1921 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
     1981 gatttccagg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
     2041 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
     2101 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
     2161 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
     2221 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
     2281 catgcttatg gtgaaggcga aaagtatgtc ccgctggtcc tccacgcgca aatgctgtgg
     2341 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     2401 ctcggtccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga ccagtggcaa
     2461 ctcaacgttg aaaagctcga cgcgcaaggg attatggccc gctggccaga aatacgcgtc
     2521 ccggacaact acatcggttt atttgaaact gattccggtt ttttgcgcag cgaactggcg
     2581 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcagctgtt caactgcccg
     2641 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggcaga cggtgagtat
     2701 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctact cccggagctg
     2761 cctgtccagc ccgtacgcaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     2821 aagaataaat tcccggcatt taccggtgag ctgcccaatg gcgatcaata ttatggtttt
     2881 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     2941 gcggatgaac gtgttccgtt tgcggaagtg gccagcgatg gttcggaagc cttcccgttc
     3001 ttgcgcaatg tgttgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     3061 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     3121 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     3181 gattttgcgc aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     3241 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     3301 gactgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     3361 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     3421 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattct
     3481 tcctgcctct acagcactca ctagaccttc cagatgctta acccactaaa cttatgaaga
     3541 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     3601 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgttttgga
     3661 tgtacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttaca
     3721 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
     3781 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     3841 gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc
     3901 gctgccgagt tattcgccca tgactcgtct tgcagcaaaa gctggacatc tcgcattgta
     3961 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     4021 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctc tccgacgctg gcgcacaggc
     4081 tgactttgcc ggtgccctgc atttttggct cgcgtggagc gttgtagtac tgtccgttat
     4141 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     4201 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     4261 aaccttcgct tccttgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga
     4321 caaagaaggt cagcacgcct ttgttaactt ccgaatccag catcttggct atagctggtt
     4381 atatggcacc ttcaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccagctgc
     4441 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaatcacg ccgaacgcga
     4501 taaacatctt cgcagtgcgg atttcctcaa taccgcaaaa tatccacagg caacattcac
     4561 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcaatc tgacgctgaa
     4621 tggtgtaacc aagcccgtca cactggaagc gaaattaatt ggtcagggtg acgacccgtg
     4681 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
     4741 caagacagat ttaggtccag cgtctcagga agtggatctg attatttcag tggaaggcgt
     4801 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     4861 tgttggatca ggaatgccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     4921 attgccattt tcacgtcctg cccgacggcg tcgctgttcc tctggcggta acgcgctttc
     4981 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcacatactg gacactgaat
     5041 aaacagcaga tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcaccgca
     5101 ctggtggcag tgcgcgataa tctcatcaga tatacgttcg cccatccgct cgtcaaaaac
     5161 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     5221 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
     5281 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     5341 ctgcatcatc tcaactgctt ttggcagctg ctcacggaag gtatctgccg gtatttccag
     5401 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaacaatgc
     5461 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
     5521 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatacg
     5581 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
     5641 agccggatcg aaggcataga gctgtgcgcg aaatgtttca acattgctcg caggtacgct
     5701 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
     5761 aaacagttga tataaagcgt cacgggtcac cttaggatcg gcaatgtgga aatacttata
     5821 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
     5881 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
     5941 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
     6001 tagcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagtcaca
     6061 aattttggtt gcggacgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
     6121 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
     6181 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccggtta
     6241 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
     6301 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
     6361 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggcatggcgt
     6421 ggttctggcc ggaccgccga atcgcccgct ggacggaagt gatcggcatg gaacacattc
     6481 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
     6541 agctgggtgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
     6601 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
     6661 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
     6721 acgcaccgga tcatgattac ggcccgcgct caagcgtttt cgtcccgttg tttgccgttg
     6781 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
     6841 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcagttgatt atactgccgc
     6901 cagagtgttc tccgccgctg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
     6961 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
     7021 cacgcccgga aggcgttcct tcacgctatt aaatcttcca tgctggatgc ttcagaatgg
     7081 catccggcat taccacagaa aatccccctg atttagcgat aaaagctctc tggattgcgc
     7141 cccctggaag tcgggcgcat aattagtgtg cttatctttt cttcttatgt tcaccgcgcc
     7201 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
     7261 aacgaaacct gatcgtcgcc tggctaggct gttttcttac cggtgccgcc ttcagcctgg
     7321 taatgccctt cttacccctc tacgttgagc agcttggcgt taccggccac tccgccctga
     7381 atatgtggtc cggtattgtc ttcagcatta catttttatt ttcggccatc gcctcaccgt
     7441 tttggggtgg actcgccgat cgtaaaggcc gaaaactcat gctattacgc tctgctctcg
     7501 gcatgggcat cgtgatggtg ttgatgggac tggcacaaaa tatctggcag tttttgatcc
     7561 tgcgggcgct tcttggttta cttggcggat ttgtccccaa cgctaatgct cttatcgcca
     7621 cacaagtacc gcgtaataaa agcggctggg cgctgggtac gctctccaca ggcggcgtta
     7681 gcggcgcgtt gctcggccca atggctggcg gtttgctcgc cgatagctac ggcttacgtc
     7741 cggtattctt tattaccgcc agtgtgctca tactctgctt tttcgtcacc ctgttttgca
     7801 tcagagaaaa attccagccg gtcagcaaaa aagagatgct gcacatgcgg gaagtggtga
     7861 catcacttaa aaacccgaaa ctggtactca gcctgtttgt caccacgtta atcatccagg
     7921 tggcgacggg ctcaattgcc cccattctga cgctgtatgt ccgcgaactg gcgggtaacg
     7981 tcagtaacgt cgcctttatc agtggcatga tcgcctcggt gccaggcgtg gcggctctgc
     8041 taagtgcacc acgactcggc aaacttggcg atcgaatcgg acccgaaaag atcctgatta
     8101 cggcgctgat cttttctgta ctgctgttga tcccaatgtc ttacgttcag acgccattgc
     8161 aacttgggat tttacgtttt ttgctcggtg ccgccgatgg tgcactactc cccgccgtac
     8221 agacactatt agtttataac tcgagcaacc agatcgccgg gcgtatcttc agctataacc
     8281 aatcgtttcg tgatattggc aacgttaccg gaccattgat gggagcagcg atttcagcga
     8341 actacggttt tagggcggta tttctcgtca ccgctggcgt agtgttattc aacgcagtct
     8401 attcatggaa cagtctacgt cgtcgtcgaa tcccccagat atcgaactga tttttcgcct
     8461 ttcatacttg caaaagcgga gaatcagcta tccttttccc tgaaacctca tcaactcaaa
     8521 gggagaatcg tgatgaccat gtacgcaacg cttgaagaag ccattgacgc tgcacgcgaa
     8581 gaatttcttg cagacaaccc cggcatcgac gccgaaaacg cgaatgtgca acagttcaat
     8641 gcccaaaaat acgttttgca ggacggcgac atcatgtggc aagttgagtt ttttgccgac
     8701 gaaggggaag aaggtgaatg tttacccatg cttagcggtg aagctgcgca aagtgtcttt
     8761 gacggcgact atgatgagat agagatccgc caggagtggc aagatgagaa tacattacat
     8821 gaatgggacg agggggaatt tcagcttgag ccaccgctgg ataccgagga aggacgcaca
     8881 gcagctgatg agtgggatga acgttaatca ctcatacggg ccatgatgaa tgtcgatcgg
     8941 cagcagtaag gtatcgaaaa ccagggagaa tggcagatca aggatcgtga cgtaacgcca
     9001 ggcggagtca cgcacgtccc attgcacacc gggataatat tggttgccat gcccctgccc
     9061 cggtatagtg cgactaatga tgctgccaca gccactcagc aaggtcacca ttatgctcac
     9121 cacaattaaa cgcatttgat acctcttccg taaaaaatac cggcataacg ccggtatttt
     9181 tattggctcg ttgtttttgc cggatgcggc gtgaacgcct tgccagaccc acaaaatctt
     9241 gcaaaatcaa taaattgcag gaacgatgta ggcctgataa gcgtagcgca tcaggcaact
     9301 acgtttttat tgcgaagccg catccggttt acgcaatgcc agtggattga gctttatccc
     9361 ttcgtaataa ctcacccatg aagaatatct ctccggggaa ttccagacgc ggaaatgcag
     9421 acgggccatc gtcaccggat cgcttagcag caccaggcga cgatcgcgat tcagcttctc
     9481 tggcgtctcg ttcagcgcct gttcaacgtg gcggtcacgg gcgatttcca gcaccttgct
     9541 ggcgcggtga cgcgcggtcg ccattgcggt tgccagagcg ttaaatgacg ggttaaacac
     9601 cgcgtgcata aaaccatcat caagggagcg ttgacgattc atctcaagga accgatcggt
     9661 atcaaccagc acctgcggcg gtgaatactc ttccgggatc aggaacagtt tccagcgttt
     9721 ggttcgcaga ccaacggtgg cacggctgga aatcaccgaa acaaacggtg acaggatcaa
     9781 cgagaagaca atcggtgcca gccagaacag aaaacgcaga tccagccacg ccatcccaac
     9841 agcccacact aaccctaaca gcagctgtga gccgtggcgt ttgaacgctt ccccccagga
     9901 agtggagtca tcatcacgct gcggcgaatt ccacactact tcccagccaa ggaacgcgct
     9961 gacgacgaag accgtatgga acagcatgcg taccggagcc agcagcacgg aaaacagcac
    10021 ttccagcagc aacgataatg taacgcgcca gaagccgccg tattctttcg ttcctttgca
    10081 ccagataagc aaaatgctca atagcttcgg caggaacaac agcaccatgg tcgaagcaaa
    10141 aagtgcaatc gccagctcag gacgccactg cggccatacc gggaacaact gccgtggttg
    10201 caggaagtat tgcggttcgg tcaacgcatg tactacctgc aatgcagtag agagcgcgag
    10261 gaacataaac cacagcggag cggagagata agacatcacg cccgtcagga acaccgcacg
    10321 gtgaaccggg tgcataccct tcaccaggaa cagacggaag ttcatcaggt taccgtggca
    10381 ccagcggcgg tcacgtttta gctcatcaag caagttaggc ggcaattctt cataagaacc
    10441 cgggagatcg taagcaatcc agacccccca acctgcacgg cgcatcaacg ccgcttccac
    10501 gaagtcatgt gacaggattg aaccggcaaa agaaccttcg cccggcagcg gagccagtgc
    10561 acagtgctcg ataaacggtt tcacgcggat aatcgcgtta tgcccccagt agtgcgactc
    10621 gccaagttgc cagaagtgca aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa
    10681 ctgctgacag cgcgcataca gcgtatccat gccggacgct ttcggcgacg actgaatgat
    10741 cccggcgttc gggttggctt ccatcaggcg caccaggccg cacaaacaat caccggtcat
    10801 taccgagtca gcatccagca ccaccatgta gctgtactgg ctgccccagc gacggcagaa
    10861 gtcatcgata ttaccgcttt tacgcttcac gcgacggcgg cggcggcgat agaaaatctg
    10921 accttcgcca ccgacttcag cgataagctc catccaggct ttttgctctg cgacgcagat
    10981 atccgggtta taactgtcac tcagaatgta gacatcaaag tgtttggcat tcccggtggc
    11041 ttttactgat tcccacgttg cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca
    11101 gataggcatg atcaacgccg tgcgatgctc cgggtttaat ggttcatcgc caactgttga
    11161 cgcagatata ctgtatttat cgcgaccaat aagcagttgc aggaagccca tcaacgccgt
    11221 ccagaacccg gcggacaccc aacagaacag taccgcaaag aggatcagga taccggtttg
    11281 cagcatataa ggcagaagct gcataaagga aacccacaca tcctgaccaa ccatatccat
    11341 aggattaatc agcgcccacc cctgataagg aagaatggtc ttcatatacc aggtcgcgac
    11401 aacagtttgc gcgagcgtca ggatcaacag aatgtaacgg cggatggtac cgacggtacg
    11461 ccacttttgc tcactctcct gctcttcttt ggtcaaacga gccagatagc gcggcgtcac
    11521 atcgcgtcca cgcaggcgat cccagaaacg gcctaccggg ttggtacgcc acgggtcggg
    11581 aaacatcgag gagcgttttg cttctggcat cgcctttagc tgatcgcgcc cttcgtcgtc
    11641 tttaattaac tgtccatcag caagtgaatc tggccaggct tgttccagac gcgcctttac
    11701 cgagccttgc ggggagtcat cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg
    11761 atgaacggcg cggatatcag tcttcggcaa tgccgctttc tcgcttgcgg cgatgggcat
    11821 tgcgtcaatg tactcagttg tcttattcat tggcaggtaa ctggtagctc caggtttcac
    11881 tcaacgtctg atcggcattc accagcgcag cacgcatttc agtggttttc ttggcatctt
    11941 tcactttcac acgcatcacc agacgccaac ctttggtaac cgggttatag cgcaccgtgc
    12001 tttcaactat ctcaccatta tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg
    12061 gcagtttttt catctcagcg ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct
    12121 ggcgaatcag gttcgactgc ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg
    12181 cgttatctgg cgcatgcagt ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt
    12241 tcatctcttt acccggctcc ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt
    12301 tggtttcatc gttagttgga atttccacca gctcaacgct gcctttgccc cactcccctt
    12361 tcggagtcac ccatgcgctt ggacgaagat cgtaacgatc atcgagatct tcaaagcggg
    12421 agaaatcacg accgcgctgc aacagaccga agccttgcgg gttttccatc gagaagctgc
    12481 tgacagctaa atgtttcggg ttattcaacg gacgccagat ccattcgcca ttaccagcat
    12541 ggatagacag accgttagag tcgtgcaact ccggacgata gttatttgca ggcgacggtt
    12601 ggttcggccc aaacaggaac atactggtta acggtgcaac ccccagtttg ccgactttat
    12661 cgcgcagata gattttcgac tgcacatcca caaccgtgtc acgtcctggc ataactacga
    12721 atttgtaagc acctgtcgcg cgcggcgagt caagcaatgc ataaatggtt aaacgtttat
    12781 cagtcggttt tggacgctcg atccagaact ctttgaagcg tggaaattct tcacccgatg
    12841 gcaaggcggt atcaattgcc aggccgcgtg cagaaaggcc ataaacctga cctgcaccaa
    12901 tcacgcggaa atagctggcc ccgagcatgc tgacgatttc atcgttttta tctttgctgt
    12961 tgatcgggta aagcactttg aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat
    13021 gctgaacatc gccgaaagtg aaataatccg ggctgtattt gattcgtttg actgcggtgg
    13081 cagtcacttc atttattttg accggggtat cgaagtacat accctgatgg tagaactcga
    13141 gtttgaatgg ggtcttcaga ttgttccagt acgctttgtc atgattaaac tggatctgct
    13201 gatagtccgc gtatttcata tcgcggaaaa cggagggcaa gttgcttttg ggcgcctcat
    13261 agcctttccc ggctaaggat tgagcttgct ttgcgacatc atcaatactg aaagcccagc
    13321 ttgaagatgt atacagggtt aacattactg cagcactcaa ccaacgcatt ttcatcattt
    13381 gtagtttatg tttcataatt agtaagcact tccccctttg tgtgcttata tcgatccgat
    13441 ctattttaat ggataacctg ccattccgac aacagaatcc cggtatcgtt cagcacactg
    13501 gctcatggat taagcacctg attgcaaccg ttttcacttt ccgtgcttct cctaaagaca
    13561 gaagcatcga cttcgtgtag ggtttgccag caaatttatt tatcaaccgt cttacagacg
    13621 cttccattct ggttagtggt agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct
    13681 ggcgcggtca ggtcggtgtc actatggcaa caagcctaaa ggcataaccc gacagggata
    13741 agacgaaaaa tctgagatca gttaaccata tatgaatcca gtacccgcgc aacgtgaata
    13801 tttcctcgac tccatccgcg cctggctgat gttgttaggg atcccttttc atatttcttt
    13861 aatctattcg agccatacat ggcatgtgaa tagcgccgag ccgtcattgt ggctgaccct
    13921 ttttaatgac ttcatccact cgttccgcat gcaggtgttt ttcgttatat ccgggtactt
    13981 ttcctacatg ctttttttac gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg
    14041 tgtaggtatc ccgatgttaa cagccatccc cttactgaca ttgccgcaat ttattatgct
    14101 gcaatacgtc aaaggcaaag cggaaagttg gcctgggctg tcattgtatg acaaatataa
    14161 tacgttggcc tgggaattaa tatcacacct gtggttttta ctggtgttag tggtcatgac
    14221 gacgctgtgc gtatggatat ttaaacgcat cagaaataat ttagaaaatt ctgataaaac
    14281 gaataaaaaa ttctcgatgg taaaattatc ggtgattttt ttgtgcctcg gcatcggtta
    14341 tgcggtaata agaagaacga tttttattgt gtatccaccc attctgagta acggcatgtt
    14401 caattttatt gtcatgcaaa cgttatttta tttgccgttc tttatcctcg gcgcactggc
    14461 tttcattttc cctcatctta aagccttgtt taccacgcct tctcgtggct gtacccttgc
    14521 agcagcattg gcgtttgtcg cttatttact caacctgcgc tatggcagtg gcgatgcctg
    14581 gatgtatgaa accgagtcgg tgatcaccat ggtcctcggt ctgtggatgg tgaatgtggt
    14641 cttctccttt ggccaccgtt tgcttaactt ccagtcagcg cgggtgactt attttgttaa
    14701 cgcatcgctg tttatctatc tggttcacca cccgttaacg ctgtttttcg gcgcatacat
    14761 tacaccacac atcacctcca actggcttgg ttttctctgt ggcctgatat tcgtagtagg
    14821 gattgcgata attctgtatg aaattcattt acgcatccca ttattgaagt ttttgttctc
    14881 tggtaaaccg gttgttaagc gtgagaacga taaagcacct gcccgttaag ccgcgtttac
    14941 aataaccatt ccacgggcaa tatcgacgcc agtttgacca taacccgctt ccagaaactg
    15001 gtggcgggtt cttttttgag aataatctct ttctttgcat gacgatcaac ccagttgatc
    15061 cgtccccacc tgtccagacg gagctgccag gccgcatcat actggctctg aataaagcgt
    15121 ttatcaatta actgtgccag cgtttcgctc tctatcacga agcccatttc agtattgagc
    15181 aatgttgaac gcggatcgaa attgaaagag ccaataaaca ccgttttacc atcgatgcta
    15241 aaggttttag catgcaggct ggcaccggaa ttaccggtta taccgcgatc gtgtaatgta
    15301 ctactttgtt cacgcgtcgg cttgagttca tataattcca ccccatagcg aagcaatttt
    15361 ttgcgccagc gcgcgtatcc ggcatggacg acagcaacat cgttagcggc aagagaattg
    15421 gttaggatcg caatctttac cccttttctc accatccgta agagttgcgc cacacctgca
    15481 cgtgtcggta caaaatagga agagataata tcgatgcgtt cactgggtga gcccatgata
    15541 tcgaacaggc gctgcggtaa cagtgaatgc cgttttgcct tgccctcccc tttcgccgga
    15601 tcatcactta ataaacatgt tttcgcccag ataagcggca acgttccatc aaccagatga
    15661 tgtataaacg gactggattc cattttgcgt aagtaacgat gcgtcatgac atcgttatgc
    15721 caggaggcgg gtaactcgat gtgatccgcc atttcacctt ccgggacatc cagcacctgc
    15781 tgtaaggggg aaaccgattt gcaataccag tagcgggcga aatcatcggc aacgtcctct
    15841 accacgggtc ctattgccat gacatctaaa tccgaaaaaa gtggctcttc ccctgcccca
    15901 aaataggcat caccaatatt tcgtcctccc accagcgtca ccacgccatc gacagtgaaa
    15961 cttttattgt gcatacggcg attgagacgg gaaaagtcgg tgatataacc aagcggacgt
    16021 agcaaacgaa acgagaaagg attaaaaagc cggacttcaa tgcgtggatg gctgtcaagc
    16081 aagcgtaaaa tgtcatcaag tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga
    16141 acgccacgct ttgctgcggc taacagggcg gaaaacagta accgacccga catatcgtcc
    16201 tgccagatgt aatactgaac atccagcgta tgctctgcca tttcagccag acgataccgg
    16261 gcggcaaacg catccagact tttctccaga ggaaaaagtc cacactgacc gggatgttgc
    16321 gaacacagtg gcagcaccgc gctcgccagc cgaggcaaat cattcatctc cttgttgggt
    16381 aaggagtctt tcgtagaggt gggcgttttc ttcatcataa cagacaaagt atacctgttc
    16441 gggtaaagcg tgacgggtaa taaattctga aacggttttt actgcgattt cagccgctgc
    16501 cgcacgggga taaccataaa ccccagtact gattgcagga aaagccactg acgtatagct
    16561 gtttgccgcc accagtcgta ggctattgag ataggcatcc tgcaaaagct ggtcttcgtt
    16621 ttgttcacca ccacgccaga ctggcccgac ggtgtgcact acggctttag cgggaagatc
    16681 gcctgcaagc gtaataacgg catgccccgt agggcaatcg ccctgctgct gcctgacttt
    16741 taaacaggca tccagcaggg ccggacccgc tgcgcgatga atggccccat cgacgccgcc
    16801 gcctcccatt aatgacggat tagccgcatt cacaatcaca tcaacggcca gtttggtaat
    16861 atcaccctgc acaacatgaa tacgcgtttt cataacgcct ccttacacct tcttgtttcc
    16921 taagtgtaca gcaggctttt tagttaaatc gcatttttct gcttagcaac gtgagttgtt
    16981 cagaaactta gttatattcg atgttcatta cagccagctt tttctgttta tcaagatact
    17041 gcacttttac tgaggctatt tgcacgtttt taacattgcc gctcattaag gctttgctgg
    17101 aataggtttt acgcggaaca gaaccattct gtgggcagtt catttctacg ggtgatgact
    17161 gggtgctgac gtcacatgcc ggttccacaa tttggccata aaaatggatc acgccccctt
    17221 gcgcggtgct tcctgcaatg gcaataaaag gaaaaaacaa actgccgagc ataagagagt
    17281 ttaaaaaagg tctgaacatt tattctattt cctcaatgat tagtcatcct tgagggttgt
    17341 gttatccata ctttcaggtt ggctgattcg cagcagacca ttctctccag gttcatctta
    17401 tgctcgatat ttcaacaaat taagactttt ctgaagaggg cggccattgt tgtgataaat
    17461 gaagtgactg tccatcagaa acagtaacaa ctattttcac ccgatcgtcc ggggaaatat
    17521 ttaaactcaa cttcgtcaaa gcaatgggtt gattagcagg caatgagagg gtcttttctt
    17581 gcttcgtctg actttgccct gaactgcctt cgcgcaggga caatatttgt actctgcaca
    17641 gacaagattg agtaagagtg acttcaggaa taatggtgta catatcccct tgctgggtcg
    17701 tattaaaggt tatctgactg gaaagtgccg caaggagtaa taacgcattc atattcttct
    17761 cccgaaaaaa aaacagggct tacgccctgt ttctttcata cagatgatgt attagtactg
    17821 atgagcggtc gcgttgttac caaagccaac ctgagtgacg ttaacgctgg agttagatgc
    17881 agtctggtca acagcagcac cgttaccgcc accgaactgt ttaacagtca tagtagaatc
    17941 tttaccattc cactgatcaa gagtagcgct gttgccgaaa ccacgctgag tcagatcgat
    18001 tgagctgtca tcagaacctt ggccaacatc tgcaccatta ccgccgccgt gctgggtaat
    18061 ggtcagatca gagttgcggg cgtcagcttg cagagcaaga gcagagttac cgccaccgta
    18121 ctggtaaata ttcagctctg aattcgggcc gctgttatta ccgccaccac cgtggttgcc
    18181 gccaccgccg ccgtactgag gaacaacacc tgccagagcg ctaccggaga atacgattgc
    18241 tgcaattgct gctactttta aaagtttcat gtaaaacccc catcggattg atttaaaagt
    18301 cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc tgcactacaa
    18361 ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt attaccagaa cctttctgga
    18421 taatcatcgc agtattacca taagcacctt gcgaaatact ggcatcgttg gcactgcccg
    18481 cctgatcaat atatgcaagg ttataatctc ctgtctggtc aatctttgcc cggttgctac
    18541 taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac tgagcactat
    18601 tattagtccc agcttgacca attatggctg cctgattaaa tgaagactta ctcaattcat
    18661 ttaccgcaaa gttatattct gaattagcta aatcataacc tgctgcggct gcaatcccag
    18721 gcgcacccag tattgttaac atcataaata acaatttgtt tttcatgttg tcaccctgga
    18781 cctggtcgta catttaagaa attaaatcat ttcaacttgg ttgttaacgc aacccgtatt
    18841 ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa atatttttaa taactcaccc
    18901 gcgcgttgta tttttctttt tttgttcata ccaaagtatt aaaacccacg taaatacgct
    18961 gttatctacg caaaaaatat tttgttttct tttaaatctc cgttttccgc tatcaaaaat
    19021 caccagacag tcattcttct tgcccgtcgc tgattgctgc gatatgtctt gcgcacaagc
    19081 cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac ggtggtatgg agaaaaaaca
    19141 agaacgtttt acatgacgaa aggactacag cgaaatattt tttatatgca ttattagtaa
    19201 gttagcacca tttgtatgat tttttaaaat tgtgcaataa aaaccaaatg tacaactttt
    19261 ctatcatttc taaacttaat aaaaccttaa gattaacatt ttaatataac gagttacatt
    19321 tagttacatg tttaacactt gatttaagat ttgtaatggc tagattgaaa tcagatgtaa
    19381 tccattagtt ttatatttta cccatttagg gctgatttat tactacacac agcagtgcaa
    19441 catctgtcag tacttctggt gcttctattt tagaggcagc tgtcaggtgt gcgaacaata
    19501 aaaaagcggg gtttcatcat gtttaatgaa gtccatagta ttcatggtca tacattattg
    19561 ttgatcacta aaccttcttt gcaagcgaca gctctcttgc agcaccttaa acaatcgctg
    19621 gcgattacag gaaaattaca taatattcaa cgttctctgg acgatatatc ttcaggctct
    19681 attattcttc tggatatgat ggaagcggat aaaaagctta tccattattg gcaggatact
    19741 ttgagcagga aaaacaacaa tatcaaaata ttgttgctaa atacgcctga agattacccg
    19801 taccgcgaca ttgaaaactg gcctcatatc aacggcgttt tttatgccat ggaggatcaa
    19861 gaacgtgttg tcaatgggtt gcaaggcgtc ctgcgcggcg aatgctactt tacgcaaaag
    19921 cttgccagtt acctgattac gcattcaggt aactatcgtt ataacagcac ggaatcagcc
    19981 ctccttactc atcgggaaaa agagatcctg aataagctgc gtatcggcgc gtctaataac
    20041 gagatcgctc gttcgttgtt catcagcgaa aatacggtta aaacgcatct ttataatctt
    20101 ttcaagaaga tagccgtaaa aaaccggaca caagcggttt cctgggcaaa cgataacctc
    20161 aggcgataaa gccatgaaac gttatttacg ctggattgtg gcggcagaat ttctgttcgc
    20221 cgcagggaat cttcatgccg ttgaggtaga agtcccggga ttgttaactg accatactgt
    20281 ttcatctatt ggccatgatt tttaccgagc ctttagtgat aaatgggaaa gtgactatac
    20341 gggtaactta acgattaatg aaaggcccag tgcacgatgg ggaagttgga tcactataac
    20401 ggtcaatcag gacgttattt tccagacttt tttatttccg ttgaaaagag acttcgagaa
    20461 aactgtcgtc tttgcactga ttcaaactga agaagcacta aatcgtcgcc agataaacca
    20521 ggcgttatta agtacgggcg atttggcgca tgatgaattt taaataataa attgttcgga
    20581 ggctgcaatg cgtgtcaaac atgcagtagt tctactcatg cttatttcgc cattaagttg
    20641 ggctggatcc atgactttcc agttccgtaa tccaaacttt ggtggtaacc caaataatgg
    20701 cgcttttttg ttaaatagcg ctcaggcaca aaactcttat aaagatccga gctataacga
    20761 tgactttggt attgaaacgc catcagcgtt agataacttt actcaggcca tccagtcaca
    20821 aattttgggt gggctactgt cgaatattaa taccggtaaa ccgggccgca tggtgaccaa
    20881 cgattatatt gttgatattg ctaaccgcga tggtcaattg cagttgaacg tgacagatcg
    20941 taaaaccgga caaacctcga ccatccaggt ttcgggttta caaaataact caaccgattt
    21001 ttaagcccca gcttcataag gaaaataatc atgcagcgct tatttctttt ggttgccgtc
    21061 atgttactga gcggatgctt aaccgccccg cctaaagaag ccgccagacc gacattaatg
    21121 cctcgtgctc agagctacaa agatttgacc catctgccag cgccgacggg taagatcttt
    21181 gtttcggtat acaacattca ggacgaaacc ggacaattta aaccctaccc ggcaagtaac
    21241 ttctccactg ctgttccgca aagcgccacg gcaatgctgg tcacggcact gaaagattct
    21301 cgctggttta taccgctgga gcgccagggc ttacaaaatc tgcttaacga gcgcaagatt
    21361 attcgtgcgg cacaagaaaa cggcacagtt gccattaata accgaatccc gctgcaatct
    21421 ttaacggcgg caaatatcat ggttgaaggt tcgattatcg gttatgaaag caacgttaaa
    21481 tcgggcggtg ttggggcaag atattttggg atcggtgcag acacgcaata ccagcttgat
    21541 cagattgccg tgaacctgcg tgtcgtcaat gtgagtaccg gtgagatcct ctcctcggtg
    21601 aacaccagta agacgatact ttcctatgaa gttcaggcgg gggtcttccg ctttattgac
    21661 taccagcgct tgctggaagg ggaagtaggt tacacctcga acgaacccgt tatgctgtgc
    21721 ctgatgtcgg ctatcgaaac aggcgtcatt ttcctgatta atgatggtat cgaccgcggc
    21781 ctttgggatt tgcaaaataa aacagaacgg cagaatgaca ttctggtgaa ataccgccat
    21841 atgtcggttc caccggaatc ctgatagata aaaacgccgg aattaataat ccggcatttt
    21901 actgtttaag cgaaagcgtg agtgcccctc acgctttatt tttgatttgc tgctcacggt
    21961 gagcggtctt cgtactacgc gccgccagcc acaggccgct gtttttcatt gcgtaaccaa
    22021 ataccagtcc aagcgccagc gaaggtaaca ccagtttcca gtccccctgc ccggcaaacg
    22081 tcgcgcatgc gcctataaac gtccccggaa caaatgaaag cagcagctgt ttggcctgaa
    22141 tacacatcag aaacgcgaca ataccggtta ttacataacc gagaatttcc agatgtggtg
    22201 ccagtgcact accgtaaata atgaccatcg cccacaccac gccactaagc agggttgcag
    22261 cggagatcgc cagccctttc agcccacctt gcgggcaggc aaagtaggcc gtacagccca
    22321 ggaagcccgc ccagcttagt aagcccaggg aaacagccac ccatccccag ataccggaga
    22381 gaatgcctgt tgtgattgca atagagagaa gtatgttcat gccgcgcatg atagcagaaa
    22441 acgcggccat gaatgagatc gagcacacat tttatgcaac aacaaattat cagttgcagt
    22501 tatgagtgat gcagatcaca tttactcttc agaatcttct tccagctcgt cccacattgc
    22561 actaatagca tcgcgggtta gcggtgccat ggtgcgccag aaaggcgtag ttgcatgggc
    22621 ctccactttg ccaaggaacg caccacacca gggtaacaga tactcactga acagtgtttc
    22681 cagtgcttcg ctctcatctt ccgttgactg atcttccagc caggaagccg caagcagcaa
    22741 tgtgccgatg tgatcggctg gcgtatccgc tagtggcatc cctcgttcgg aaagaaaagc
    22801 gcgcacttcc gcttccgttg cgccctcaac ccatgcgcta cgatatggcg gcacagcaca
    22861 ttcatcgccg ataaacaacg cattgtaatc ggcagagact tgcgtcatat cacagctttt
    22921 ctgtaaacgc gtcagtaact catcctgctc cagtggccag ttcgcagcca gtttcccctc
    22981 acgaatcagg gtaaacaacg gcaccagtaa aggatcttgc ggttggcggt aatagagcga
    23041 acccagcaca cgacagagga tagaaaactc gttcattaat atatttccat tactagtaat
    23101 taaaggtctg caaattccgc aatcggtgcc ataccgcgag attcaaggaa gttcagtaag
    23161 cggcgcggag aaacattcaa aatgcgttct ggcggaaaat ctaccgcgtc gaggatttta
    23221 agacactctt caaattcccc catggtaaat gcagtgtgag aatccgagcc taatgccacc
    23281 caacctccag cgtcacgtac cgctgcagct accgcacgac agttgtcttc actgccctta
    23341 cgtgagtgta aaaatgagga attattgatt tccagcgcca cctgatattt cgcggctgct
    23401 tcagcaacgg ctttaacatc tatctcatat ttcggatttc cgggatggct tattatatgc
    23461 acattgccgc tggcgatagt ggcgatcatc gcttgtgtat tggttgcttt gtcatgtggc
    23521 gcaaaaaccg gctcatgaaa accggcaata atcagatcca gcgaatcaaa cattttgccg
    23581 ctgcaatcaa tttcaccatc aacattttta atgttagctt cgatgccgcg caggatccct
    23641 accccatcaa ccactcgcgg ccagatacgc atgttaatga agtgccagtg atgcggcgca
    23701 tcttccatat ccgggccatg atcggtgatc gcaaaaagtt taatgccctt ttgtttggcc
    23761 tgggcaatgt aatcactcaa tgtgctatat gcatgcgtgc tggcaacggt atgcatatga
    23821 aggtcgacgg gatacataac tctctcctgt attcaattcc cgccaaggat agcaggaaac
    23881 ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc cgcagaccct ctcccctttt
    23941 tcgagctggg caatggtgcg cgaaatgtaa tccacagctt cagcgggacg ggtaatcgcg
    24001 gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga gcggactttc cggcggtaag
    24061 ggttcacgat taaaaacatc cagcattgcg cctttaactt tgccgctatc cagcgccgcg
    24121 agcaggtcat cttccacgac atgaacacca cgcgccaggt tgaggagata cgcgccatcc
    24181 ggtaattttt cgagtaattg ttgattaata atgccgacgg tttcaggggt attcggtaac
    24241 aaattaatca atacccggca ttggctcaga aatgcagaaa gttcttcccg tccggcaaag
    24301 ctttgcacgc caggccacga tttacgggtt cgactccagc aacgcagcgg gaagcgccag
    24361 gtttgcagac tctgtgcaac tttactgccc agtacgcctg cgcccaaaat gccgatggta
    24421 aaatcttccc gatgatattc aggcagcggt tgccaatgcg aactattttg ctggaggcgg
    24481 taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga cagcatattc ctgcatttgc
    24541 tcgcccatac cggtatcttc caggcgaaaa agtggaacag aaggcttcag catttcaggg
    24601 tgagcctgta gcttgctcaa aatagaatca acacctgccc caagtgcaaa caccgcttta
    24661 agatcgcgcc ctgccagcat ttcaacagga ggatgccaga ctaaagcata atcggctgat
    24721 tcattgtctc cgcttttcca tgctctgact cttgcctgag gaatagcttt gtgcagtgcc
    24781 tcaatccacc attgggtatc gaacgttggg tgataaaaga tgatatccat actgactccc
    24841 gaaaagcgtt gcgcgaaatt tattcgcact tatcgttatg atctacaaag gccaccagca
    24901 taacaaatcc gtggtcggtg acaaaaaaag caaatttcgc ttattaaaac tac
//
LOCUS       DAOFSV010000055        24828 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.055, whole genome shotgun sequence.
ACCESSION   DAOFSV010000055 DAOFSV010000000
VERSION     DAOFSV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.055"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..529)
                     /locus_tag="QBH27_002397"
     CDS             complement(<1..529)
                     /locus_tag="QBH27_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ7014700.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVK"
     gene            complement(662..1309)
                     /gene="grxB"
                     /locus_tag="QBH27_002398"
     CDS             complement(662..1309)
                     /gene="grxB"
                     /locus_tag="QBH27_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDQ7014701.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(1373..2581)
                     /gene="mdtH"
                     /locus_tag="QBH27_002399"
     CDS             complement(1373..2581)
                     /gene="mdtH"
                     /locus_tag="QBH27_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDQ7014702.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            2817..3401
                     /gene="rimJ"
                     /locus_tag="QBH27_002400"
     CDS             2817..3401
                     /gene="rimJ"
                     /locus_tag="QBH27_002400"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDQ7014703.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            3412..4059
                     /locus_tag="QBH27_002401"
     CDS             3412..4059
                     /locus_tag="QBH27_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDQ7014704.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            4061..4984
                     /locus_tag="QBH27_002402"
     CDS             4061..4984
                     /locus_tag="QBH27_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ7014705.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFGVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVDCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            5094..6629
                     /gene="murJ"
                     /locus_tag="QBH27_002403"
     CDS             5094..6629
                     /gene="murJ"
                     /locus_tag="QBH27_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDQ7014706.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(6669..7085)
                     /gene="flgN"
                     /locus_tag="QBH27_002404"
     CDS             complement(6669..7085)
                     /gene="flgN"
                     /locus_tag="QBH27_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDQ7014707.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPYRGSKKISI"
     gene            complement(7090..7383)
                     /gene="flgM"
                     /locus_tag="QBH27_002405"
     CDS             complement(7090..7383)
                     /gene="flgM"
                     /locus_tag="QBH27_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDQ7014708.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(7459..8118)
                     /gene="flgA"
                     /locus_tag="QBH27_002406"
     CDS             complement(7459..8118)
                     /gene="flgA"
                     /locus_tag="QBH27_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDQ7014709.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            8273..8689
                     /gene="flgB"
                     /locus_tag="QBH27_002407"
     CDS             8273..8689
                     /gene="flgB"
                     /locus_tag="QBH27_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDQ7014710.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            8693..9097
                     /gene="flgC"
                     /locus_tag="QBH27_002408"
     CDS             8693..9097
                     /gene="flgC"
                     /locus_tag="QBH27_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDQ7014711.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            9109..9804
                     /gene="flgD"
                     /locus_tag="QBH27_002409"
     CDS             9109..9804
                     /gene="flgD"
                     /locus_tag="QBH27_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDQ7014712.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            9829..11034
                     /gene="flgE"
                     /locus_tag="QBH27_002410"
     CDS             9829..11034
                     /gene="flgE"
                     /locus_tag="QBH27_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDQ7014713.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDSLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWDVYTLDSSDP
                     TGTANPATTLVFNANGVLTSDPTKDITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            11054..11809
                     /gene="flgF"
                     /locus_tag="QBH27_002411"
     CDS             11054..11809
                     /gene="flgF"
                     /locus_tag="QBH27_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDQ7014714.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            11981..12763
                     /gene="flgG"
                     /locus_tag="QBH27_002412"
     CDS             11981..12763
                     /gene="flgG"
                     /locus_tag="QBH27_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDQ7014715.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            12817..13515
                     /gene="flgH"
                     /locus_tag="QBH27_002413"
     CDS             12817..13515
                     /gene="flgH"
                     /locus_tag="QBH27_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDQ7014716.1"
                     /translation="MQKNAAHTYAISSLLALSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            13527..14624
                     /gene="flgI"
                     /locus_tag="QBH27_002414"
     CDS             13527..14624
                     /gene="flgI"
                     /locus_tag="QBH27_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDQ7014717.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            14624..15565
                     /gene="flgJ"
                     /locus_tag="QBH27_002415"
     CDS             14624..15565
                     /gene="flgJ"
                     /locus_tag="QBH27_002415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDQ7014718.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPIKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            15631..17274
                     /gene="flgK"
                     /locus_tag="QBH27_002416"
     CDS             15631..17274
                     /gene="flgK"
                     /locus_tag="QBH27_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDQ7014719.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDTSAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            17286..18239
                     /gene="flgL"
                     /locus_tag="QBH27_002417"
     CDS             17286..18239
                     /gene="flgL"
                     /locus_tag="QBH27_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDQ7014720.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(18434..21619)
                     /gene="rne"
                     /locus_tag="QBH27_002418"
     CDS             complement(18434..21619)
                     /gene="rne"
                     /locus_tag="QBH27_002418"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDQ7014721.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            22192..23151
                     /gene="rluC"
                     /locus_tag="QBH27_002419"
     CDS             22192..23151
                     /gene="rluC"
                     /locus_tag="QBH27_002419"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDQ7014722.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            complement(23263..23847)
                     /gene="yceF"
                     /locus_tag="QBH27_002420"
     CDS             complement(23263..23847)
                     /gene="yceF"
                     /locus_tag="QBH27_002420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDQ7014723.1"
                     /translation="MPELILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            24046..24567
                     /gene="yceD"
                     /locus_tag="QBH27_002421"
     CDS             24046..24567
                     /gene="yceD"
                     /locus_tag="QBH27_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDQ7014724.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            24619..24792
                     /gene="rpmF"
                     /locus_tag="QBH27_002422"
     CDS             24619..24792
                     /gene="rpmF"
                     /locus_tag="QBH27_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDQ7014725.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
BASE COUNT         5930 a         6323 c         6678 g         5897 t
ORIGIN      
        1 gcttcacttc aatgcctttc gccagtttct tcgccattgc ttcgccctgg ctgccatctt
       61 cgcgcaggac gtaagcaggt tgctggttaa agtaattgcg taatgcctgg tttaaatagg
      121 gaagcaacgt ttgcatcacc gtttgcattt tttccggttg taccgtcgca tcgaccactt
      181 ccatctcttt caggaagatc gcgccttttt ctttatcaaa cacgggcagc gctttcagct
      241 tcagtttcat ggtcgctttc tggctaccga acagggagtt catgtccaga ttggcgtctc
      301 cggttagggt aaccttattc ggctcttcgc gaccaatttg gctggtcagg tttgtcagaa
      361 cgatatgggc gtcagccacg ccgggtaaac cgatatcttt tgagaaatta ttatgtttcg
      421 caagcgactg gttaatttct tgttcggtga tggtgtattg ggtgagttga ttacagccaa
      481 cgagcaggcc actgacgatc aatgcagcgg caaataaaaa cttgttcatg gtagtcctcg
      541 acatgaaatc tgcgtcaata tcctgacaca acgcagcatg tgtcaccagc gataacgcgc
      601 cagcagaaaa aactgaaaac ggcggcaacc cgcgaataca ggctgccgcg acgggtcagg
      661 attaaatcgc cattgatgat aacaaattga tttgtgtctg tttcgccata ttatcgcggt
      721 aatcagcaac gcggcttggc cagttgattc cggctactag cgtcagatta cgcaggagcg
      781 gaaatagctg aatatcatct tccgaaagtt cgccattcac ggcgttcggt ttgacgatca
      841 gtttgtccag cgcacgtaaa tcatcgctga tattcttaat cagaccgtca gagtgggcca
      901 gcaggtcggc aaaattaccc gcgctggcct ctttcttgtc gacgaaatat ttgcgcgcgg
      961 caggagtaga aaactcatca aatgccgatt tggcaaaacg cggcaacagc agtttgttgg
     1021 cgtagccatt gaccttgcgc agccactctt caatggcagg ggaacgtttg ccggtcagta
     1081 acggtttgcc gtcgagttta tcgacatagt gaacgatgtc catgctttct ggcatatagc
     1141 ggctgtcatc tttttgcaga atgggaacct gtttttgacc gaccatccgg gtgggtgttt
     1201 ctgcgtcgtc gttgagcaga acatgtaatt cgacggggat atttttcagg ccgaaaatca
     1261 tgcgggcttt gaggcagtaa gggcagtgat cgtaaatgta tagcttcacg tgactcctcc
     1321 atttggctgt cagttctctt tcagtatgga ggagtcaggg gggagtatca aatcaggcgt
     1381 cgcgttcaag caaacgacgc gcggcgcgtt tctggctaaa ctgccaaccc agcgcaagga
     1441 aagtgaagat gccaataatg cccagcatca tccacggaag ctctggctgg tgcgccgatt
     1501 tgcccaggtc aaacagccag ccgccaccga tataaccaat agcgccgcca atcgccagac
     1561 ccagacggct aaatcccata tagctgccgc gagctcttgc gtctgccagc gaagcactta
     1621 aggtttcacg cgcaggctcg gcaatgatcg acccgatata aaacagacaa atcagggtga
     1681 aaagttgttg caggccgctg accatgccca ctggcatcat gcttaatgac attatcaaca
     1741 gcccagccat taaccggtgt tccagacgaa aatgcttttc actccagcgg gcgatagggt
     1801 agagcaacgt taacgacaga cacgcttcaa tggcatacat ccatttaacg gcagagggcg
     1861 cgccagccac gtcgttgacc ataattggca gcatcagcat cacttgtaca gccagcatgt
     1921 agtaacccgc cagcgtcagg acataggtga caaaacgctt gtcgcgcatc acgcgggtca
     1981 tgccttcgcg aacgggcgtg cgtacggtgg agagtttcca tgctggtaac aaccacgcat
     2041 tgaacgccgc acacagcaca aacaaaactg cccctgtggc gcagaccagg cgaaagtcgt
     2101 attgcaacag ccagctcccc aacaatgcgc caatgaccgc accggcactg tcctgcatca
     2161 tcaacagcga gaaaaaacga ccacgctgtt gtggacggat tagtttcacc accagcgccg
     2221 aacgcggagg atcaaacaac gtgccgccga gtcccgaaag caggcatgaa aaccacagta
     2281 gccacggttc gtgggcgata cccattgtgg cgaatccggc ggcacgcatc agcataccgg
     2341 tgacaatcat cggtttggca ccaaagcggt cggcaattgc gccgccgaaa atacccagac
     2401 cttgctgaat aaactggcgt agaccgaggg caataccgac catgacggcg gcccagccca
     2461 tttgatcaac gaagcggata gagatcagcg ggaagacaac aaagaacccc agcacgacca
     2521 gcatattatc gatgagcagg aaatatttac ccaggttcct cgcctgcgac acgcgggaca
     2581 tttcccctcc cgggaaataa aagatgagca ctttctattc tgcattgccg ctaagacttt
     2641 tcccatcact tcggggacaa tattttttta tcaaaacgtc gctttgatag agagttttta
     2701 tcaaatatgt gaataatgca gaaaatggca ttctggactt ttcacagggt ctggttgcgc
     2761 aggtatagta atacttacag cgtattaaag acgttacggg aaggagtagg tatagaatgt
     2821 ttggctatcg cagtaacgtg ccaaaagtgc gcttaaccac agaccgactg gtcgtgcgtc
     2881 tggtgcatga tcgtgatgcc tggcgtctgg cggattatta cgcagagaat cgccatttcc
     2941 tcaagccctg ggagccagtg cgcgacgaaa gccactgtta tccatcaggc tggcaggcca
     3001 ggctagggat gattaacgaa tttcataaac aaggttcagc tttctacttt ggcttattcg
     3061 acccggacga aaaagagatt attggcgttg ccaatttttc caatgttgtt cgcggctctt
     3121 ttcatgcctg ctatctcggt tattcgattg ggcaaaaatg gcagggcaaa ggactcatgt
     3181 ttgaagctct gagcgcagcc attcgttata tgcagcgcac ccaacatatt catcgcatta
     3241 tggctaatta tatgccgcac aataaacgca gcggtgattt actggcgcga ctgggttttg
     3301 aaaaagaagg ctatgcgaaa gactatctgt tgattgatgg acaatggcgc gatcacgtac
     3361 tgacggcatt aactacccca gactggacgc ccggccgcta aggagaatct catgaaatat
     3421 caactgaccg ccctggaagc ccgcgtgatt ggttgcttgc tggaaaaaca ggtgaccacg
     3481 ccggagcaat acccgctctc cgtaaatggt gtagtcacgg cctgtaatca gaaaacgaac
     3541 cgtgaaccgg ttatgaatct gagtgaatct gaagtgcagg aacagctgga taatctggtc
     3601 aaacgtcatt atctacgcac ggtgagcggt tttggtaatc gggtcaccaa atatgagcaa
     3661 cgtttttgta attcagaatt tggcgatctg aaactgagcg ccgcagaagt ggcgttaatc
     3721 accacgctgt tattgcgtgg tgcccagacg ccgggtgaac tgcgcagccg cgccgcgcga
     3781 atgtatgaat tcagcgatat ggcggaagtg gagtcgacgc tggaacaact ggcgaatcgc
     3841 gaagatggtc cttttgtggt gcgtctggcc cgcgaaccgg gtaaacgcga aagccgctac
     3901 atgcatcttt tcagtggtga ggttgagaat ccgccggcgg taacggatat gtcgaacgcg
     3961 gttgacggtg atttacaggc ccgcgtcgaa gccctggaaa tcgaagtggc agaactgaaa
     4021 cagcgtcttg attcgttgct ggcccatctg ggagattaaa gtgaaaaaat tacgtatcgg
     4081 cgtagtgggg ttaggcggca tcgcgcaaaa agcgtggtta ccggtgctgg cggcagcgtc
     4141 tgactggacg ttacaaggtg cctggtcgcc tacgcgcgcc aaagccctgc caatttgtga
     4201 cagctggcgc attccttatg ctgattcgtt atccagcctt gccgccagtt gcgatgcggt
     4261 ttttgtgcat tccagcaccg ccagccactt tggcgtggtc agtacgttac tcaatgcggg
     4321 ggtacatgtc tgtgtcgata aaccgctggc agaaaatctg cgcgatgctg aacggctggt
     4381 ggaactggcg gcgcggaaaa aactgacgtt gatggtcggt tttaaccgtc gtttcgcacc
     4441 actctacggt gagttaaaaa cgcaactcgc caccgcgtcc tcgctaagaa tggataaaca
     4501 tcgtaccaat agcgtcgggc cacacgatct ttatttcaca ttgctggatg attatttgca
     4561 tgtggttgat accgcgctgt ggttgtctgg cggcaatgca actctcgaaa gcggtacgtt
     4621 gctgaccaat gagtccggcg aaatgctgtt tgccgagcac catttttcgg ctggtccttt
     4681 gcagatcacc acctgtatgc atcgccgggc cggaagtcag cgtgaaaccg tgcaggccgt
     4741 gactgatggt gcgctgatcg acattacgga tatgcgcgaa tggcgtgagg agcgcgggca
     4801 gggcgtagtg cataaaccga ttcctggttg gcagagtacg cttgagcaac gtggatttgt
     4861 cgactgtgcg cggcacttca ttgaatgcgt gcaaaatcag acagttccgc aaacagccgg
     4921 cgaacaggcc gtgctggcgc aacgtatcgt tgacaagatc tggcgcgatg cgatgagtga
     4981 ataaaccctg taacatctgg cggtagcaat tcaccataat ccaggtagac tattcgcctc
     5041 tttcagcgcc tgccttgcag gcgttttgcc cgtgggtctg gattagaaca ccgatgaatt
     5101 tattaaaatc gctggccgcc gtcagctcga tgaccatgtt ttcgcgtgtg cttggcttcg
     5161 cacgagacgc aattgtcgcc agaatctttg gcgcagggat ggcaaccgac gcctttttcg
     5221 tcgcttttaa acttcctaac ttgttacgcc gtatctttgc cgaaggggca ttttcccagg
     5281 catttgtacc gattctggca gaatataaaa gcaagcaggg tgaagacgcc acgcgggtct
     5341 ttgtctctta tgtttctggc ctgctgacac ttgcgctggc ggttgtgacg gtcgctggca
     5401 tgctcgctgc accgtgggtg atcatggtga ccgcgccagg cttcgctgac actgccgaca
     5461 aatttgccct gaccagccag ctactaaaga taacctttcc ctatatcttg ctgatctccc
     5521 tggcgtcgct ggtgggagcg attctgaata cctggaaccg cttctcaatt ccggcgtttg
     5581 ccccgacatt gctcaatatc agcatgattg gttttgcgct gtttgccgcg ccgtacttta
     5641 atccaccggt gctggcgctg gcctgggctg ttacggtcgg cggcatcctg cagctggtgt
     5701 atcagctacc tcatctgaag aagatcggca tgctggtttt gccacgcatt aacttccacg
     5761 atgccggagc aatgcgtgtg gtgaaacaga tgggaccggc aatccttggc gtctctgtga
     5821 gccagatctc cttaatcatc aacaccattt ttgcctcgtt tcttgcttcc ggttcggtgt
     5881 cgtggatgta ttacgccgac cgcttaatgg agtttccgtc cggtgtgctg ggcgtggcgc
     5941 ttggcaccat tttgctaccg tcgctgtcga aaagttttgc cagtggcaat catgatgaat
     6001 acaaccgttt gatggactgg gggctgcgtc tttgtttcct gttggcgctg ccaagtgcgg
     6061 ttgcgttggg cattctttcc ggtccgttaa ccgtttcgct gttccagtac ggtaaattta
     6121 ccgcctttga tgcgctgatg acccagcggg cgttaattgc ctactcggtg ggtttgattg
     6181 gcctgattgt agtgaaagtg ttggctcctg gcttttattc ccgccaggac attaaaacgc
     6241 cagtgaaaat tgccatcgtt acgctgattt taacgcaatt gatgaacctg gcgtttattg
     6301 gtccgttgaa acatgccggg ctgtcacttt ctattggtct ggcggcgtgt ctgaatgctt
     6361 cgctgcttta ctggcagttg cgtaagcaga aaatctttac tccgcaaccc ggctggatgg
     6421 cgtttctgtt gcgtctggtg gtggcggtac tggtgatgtc tggcgtgctt ttaggtatgt
     6481 tacatatcat gccggagtgg tcattgggta ccatgccctg gcgtttactg cgtttaatgg
     6541 cggtcgtgct ggcggggatt gccgcgtact tcgctgcact ggcagtactg ggcttcaaag
     6601 ttaaagaatt tgcccgccgg acggtgtaac gatacattcc ggcctgcagt gcaggccgga
     6661 gataatcttc agatcgaaat ctttttactg ccgcgatagg gggttgaggt ctgaccgttc
     6721 gccccataca gcgtcggttc ctgatgcggt ttcaacattt ccagcgcctg ttgattgcgc
     6781 tcaatctgtc cttccagtaa ccagccgtta tgttgattca tttggcgtag ttgctgcgtt
     6841 ttcacagcaa tttcctgcca gcgttgacta atatcaacgc tatttgctgt attgggttct
     6901 ttcctgcgta actgttcgag gtaatccagc gtcgccagca gtgagctttt ttgttctgta
     6961 atccattgca actggctgcc gttgatctgc cccatagaga gatgttgctg ctcttgatcc
     7021 attaccgttt tgagatcgtt aagcacagcg gacatctggt cgaggatctc tgcaagacgc
     7081 gtcatacgat caattactct gcaagtcttg ctgcgcttcg ttgatcagcg catcggcaat
     7141 tttgccagtg tccattttta gttcaccgtt acgaatcgcc agctttaacg cttcgacacg
     7201 ttcaagattg atatcactgc tgccgggttg catcagtttt gcttgcgcgt cgcttaacgt
     7261 cacactggtg ctggtggagg ctgttgtttt tgccgctcgg gtgtttgtta ccggcgcgtc
     7321 agtggtttcg cgcggttgaa cggtgcttac tggcttcaga ggcgaagtgc gatcaatact
     7381 catggtttat tcctcattga gggcgctttt atcatgtgtt gcttatttat cggcaaggga
     7441 cgggtaatct ttaacagctt acaggtttat aagaatattc ccatctgcat caacaacgcc
     7501 gctgaccact tgtcccgata tcatgcgcac ccgcgcattc tgtgcgacgg ctgcattgtt
     7561 cagcgcctga ccttctgcgt tggcgctaaa cccatcaccg ctggcgataa cgttgacgcg
     7621 ttgtcccgct tttacccgcc atgcctggcg aaagtgggtt aactggatag gttgatcggg
     7681 tgatagatcg cgcaggctaa cggcatcaac aagttgattg atatccagca ccgtacgcgg
     7741 tggcaaggta tccagccgtc cgcgtttcag tttgacattg ccagcttcca gctttcctcc
     7801 acgcacaatg ggcatcgcgg caaccacata atttcctgtg gcctgtacat taacctgtaa
     7861 atatcgtttg tcgttaccgc agcgtgccaa cacattcaca ttgccccaca ggcgggaatt
     7921 attgctcatc gaaagcaatg gctgctcgca cggcggtagc agattgggcg ctgtacgaat
     7981 agaaacacga acctcatcac ttacccccgc gagttgggcg ctaaaaaagt tgtgcaattg
     8041 cgatgtgaga ttgctcgccg tacttaacgg actgaagagt atcgcgatga tcgccacgct
     8101 acgttttatt gtcagcattt tcgcccccag ccatttctac aacgtgaatt gtacctgtcc
     8161 gcaatgacca tcaacggcat aaatagcgac ccattttgcg tttattccgc cgataacgcg
     8221 cgcgtaaagg catttaagct gatggcagaa ttttgatacc tgcggaggag atatgctcga
     8281 taagctcgac gccgccttac gttttcaaca agaggcgctc aatctgcgcg cccagcgtca
     8341 ggaagtgctg gcagcaaaca tcgccaatgc cgatacccct ggttatcagg cgcgcgatat
     8401 cgattttgcc agtgaactta aaaaagtcat gcaacgtgga cgggatgcaa ccagtgtggt
     8461 tgctctgacg atgacctcaa cgcaacacat tccggcgcag gcgctgacgc ctccttccgc
     8521 agaactgcaa taccgtattc cggaccagcc ttcgcttgac ggtaatactg tcgatatgga
     8581 tcgcgaacgc acccagtttg ccgataacag cctgcaatac cagatgagcc ttagcgcgtt
     8641 gagcgggcaa attaaaggca tgatgaacgt tttacagagc ggaaattaac ggatggcact
     8701 gctgaatatt tttgatatcg ccgggtcggc gttaactgcc cagtcccagc gtctgaacgt
     8761 ggcggccagt aatctggcga atgctgatag cgtgaccggt cccgatggac agccatatcg
     8821 ggcaaaacag gtggtattcc aggttaacgc tgcaccaggt gctgcgacag gcggcgtaaa
     8881 ggttgccgat gttatagaaa gtcaggcccc ggacaaactg gtttatgaac cgggtaatcc
     8941 gctggcggat gcaaagggct acgtgaaaat gccaaacgtt gatgtcgtcg gggagatggt
     9001 taacaccatg tcggcgtcac gcagttatca ggccaatgtt gaagtgctca acacggtgaa
     9061 aagcatgatg ctgaaaaccc ttacgctcgg tcaataaagg agaaagctat gtctattgcg
     9121 gtaaccacca ccgatccgac gaataccggc gtcagtacca ccagcagtag ttcgctcacg
     9181 ggcagcaacg ccgcagattt acaaagcagt tttctgactt tgttggtggc gcagctgaaa
     9241 aaccaggacc cgaccaatcc aatggaaaac aacgagctga cgtcgcaatt ggcacaaatc
     9301 agcacggtca gtgggattga aaaactcaat accacgctcg gatctatttc cggacagatt
     9361 gataacagtc agtcgttaca ggccagtaac cttatcggtc acggcgtgat gatccccggt
     9421 accacagttc ttgcgggaac cggcagtgaa gaaggggctg tgaccacgac cacgccgttt
     9481 ggtgttgagt tgcaacaggc agcagacaaa gtgacggcca ccatcaccga taaaaacggc
     9541 gcggtcgtcc gtaccattga tatcggtgaa ctgactgccg gagtccacag tttcacctgg
     9601 gacggtacgt tgactgacgg cagcactgcg ccgaacggtt cttacaatgt agcgattagc
     9661 gccagtaacg gtggtacaca actggttgcc caaccgctgc agtttgctct ggtgcagggg
     9721 gtgatccgcg gcaacaacgg taatacgctg gatcttggca cttacggcac cactaccctc
     9781 gacgaagtac ggcagataat ttaagccttc acatttcagg agtcagtcat ggccttttct
     9841 caagcggtta gcggattaaa cgctgccgcc accaacctcg atgttattgg caacaatatc
     9901 gccaactccg ccacctacgg ctttaaatca ggcacggcct cttttgccga tatgtttgcc
     9961 ggttcgaaag tgggactggg ggtaaaagtt gctggtatca ctcaggactt taccgatggc
    10021 acgaccacca acaccgggcg tggtctggac gttgctatca gccagaacgg ttttttccgt
    10081 ctggtagaca gcaatggttc ggtattctac agccgtaacg gacaatttaa gctggatgaa
    10141 aaccgtaacc tggtgaatat gcaaggttta cagctgacgg gttacccggc aaccggtacg
    10201 ccgccgacta ttcagcaagg ggcgaatccg accaatattt cgattccgaa taccctgatg
    10261 gcagcgaaaa ctaccaccac ggcgtcgatg cagatcaacc tgaattccag tgattcgctt
    10321 ccctctgtta acgcatttga tgccagcaat gcggatagct ataacaaaaa aggttcggtg
    10381 actgttttcg atagtcaggg taatgctcat gacatgagcg tctactttgt gaagaccggg
    10441 gataataact gggatgttta caccctggat agcagtgatc caacaggcac agctaaccct
    10501 gcaacgacgc tggtgtttaa tgccaatggc gttctgacct cagatccaac aaaggatatt
    10561 accaccggcg caattaacgg cgcagatccc gccacgttta gcctgagctt cctcaactcc
    10621 atgcagcaaa ataccggcgc taacaacatt gtggcaacca cccagaacgg ctacaaaccg
    10681 ggcgatctgg tgagttatca aatcaatgat gacggtacgg ttgtcggcaa ctattccaac
    10741 gaacaaaccc aactgctggg gcagattgta ctggcgaact ttgccaacaa cgaaggtctg
    10801 gcatccgaag gcgacaacgt ctggtccgct acgcaatctt ctggcgtggc gctgttgggg
    10861 acagccggga cgggcaactt tggcaccctg accaacggtg cgctggaagc gtccaacgtc
    10921 gatctcagta aagaactggt caatatgatc gttgcccagc gtaactatca gtctaacgcc
    10981 cagaccatca aaacccagga ccagatcctc aatacgctgg ttaacttacg ctaatcgctg
    11041 acgggatagc tcaatggatc acgcaattta taccgcgatg ggagcagcca gccagacgct
    11101 gaatcaacag gcggtaaccg ccagtaatct ggccaatgcc tctacgcccg gttttcgcgc
    11161 gcagttaaac gccttgcgcg cagtgccggt ggaagggctt tcactgccca cgcgcacgct
    11221 ggtaacggcg tcaacaccgg gtgcagacat gacgcccggc aagatggatt acacctcgcg
    11281 cccgctggac gttgcgttgc agcaggatgg ttggctggtc gtgcagaccg ctgacggcag
    11341 tgaagggtat acgcgtaatg gcagcattca ggttgatccc accgggcaac tgacaattca
    11401 gggacatccg gtggtaggcg aggcggggcc aattgctgtg ccggaagggg cggaaatcac
    11461 tattgctgcc gatggcacaa tctcggcgct gaatccgggc gatccggcaa atacggttgc
    11521 gccagtgggg cgtctgaagc tggtgaaagc cacgggcagc gaagtccagc gcggtgacga
    11581 tggcattttt cgtttaagcg cagaaagcca ggctacgcgt gggccggtac tgcaggcaga
    11641 tccaaccttg cgtgtgatgt cgggggttct ggaaggcagt aacgtcaatg ccgttgcggc
    11701 aatgagcgac atgattgcca gcgcacggcg ttttgaaatg cagatgaagg tgatcagcag
    11761 cgtcgatgat aacgcaggcc gtgccaacca actgctgtcg atgagttaat tgaaaggata
    11821 catgacaagt ataagttgcc ctatgcgcaa gtttatcggg tctatggggg caatcgcaat
    11881 ttatcgattt tgcgagctct tgtaggccgg ataaggcgtt tacgccgcat ccggcaagaa
    11941 gacatatgca ctttgtcact aatccactac aggacatttt atgatcagtt cattatggat
    12001 cgccaaaacg ggccttgacg cccagcaaac caatatggac gtcattgcca acaacctggc
    12061 aaacgttagt accaacggtt ttaagcgtca gcgcgcggtg tttgaagatc tactttatca
    12121 aaccattcgc cagccggggg cacagtcttc cgaacaaacc accttaccat ccggattaca
    12181 aatcggtacg ggggtacgcc ctgtcgccac ggaacgttta cacagccagg gcaacctgtc
    12241 gcaaaccaat aacagtaaag acgtcgcgat taaagggcag ggctttttcc aggtgatgtt
    12301 gccagatggc tcatcagcct atacccgtga cggctctttc caggtggatc agaacgggca
    12361 gctggtgacg gccggtggtt ttcaggtgca gccagcgatc accattccgg cgaatgcgtt
    12421 aagtatcacc atcggtcgtg atggcgtggt cagcgtaacc caacaaggcc aggcagctcc
    12481 ggttcaggtt gggcagctca atctcaccac ctttatgaat gacaccggac tggagagcat
    12541 tggcgaaaac ctctacaccg aaacgcaatc ctccggtgca ccgaacgaaa gcacgccggg
    12601 cctgaacggc gcgggactgc tgtatcaagg gtatgttgaa acgtctaacg tcaacgtggc
    12661 ggaagaactg gtcaatatga ttcaggtgca acgcgcttac gaaatcaaca gtaaagcggt
    12721 gtccaccacc gatcagatgc tgcaaaaact gacgcaactc taaggcttaa ccggtgggca
    12781 ggttcaccgg tttactgatt tttgaagatg atagccatgc aaaaaaacgc tgcgcatact
    12841 tatgccattt ccagcttgtt ggcgctttca ctaaccggct gcgcctggat accctccacg
    12901 ccgctggtac agggggcgac cagtgcacaa ccggttcccg gtccgacgcc cgtcgccaac
    12961 gggtctattt tccagtctgc tcagccgatt aactatggct atcaaccgct gtttgaagat
    13021 cgtcgaccac gcaatattgg cgatacgctg actatcgtgt tgcaggagaa cgtcagcgcc
    13081 agcaaaagct cctctgcgaa tgccagccgt gacggtaaaa ctaattttgg ctttgatact
    13141 gtgccgcgct atttgcaggg gttgtttggt aacgctcgtg ccgatgtcga agcctccggt
    13201 ggtaacacgt tcaacggaaa aggcggggcc aatgccagca atacctttag cggtacgttg
    13261 acggtgacgg ttgaccaggt actggtcaac ggcaacctgc atgtggtggg tgaaaaacag
    13321 atcgccatta atcagggtac cgaatttatt cgcttctctg gggtggttaa tccacgcact
    13381 atcagcggca gcaataccgt accgtctact caggtggcag atgcgcgcat tgaatacgta
    13441 ggcaatggct acattaacga agcgcaaaat atgggctggt tgcagcgttt cttccttaac
    13501 ctgtcgccaa tgtaagtgag gctgtggtga ttaaatttct ctctgcatta attcttttac
    13561 tggttaccac ggcggctcag gctgagcgta ttcgcgatct caccagtgtt cagggggtaa
    13621 ggcaaaactc actgattggc tatggcctgg tggtggggct tgatggtaca ggtgaccaga
    13681 caactcagac gccgtttacc acacaaacgc ttaataacat gctctcacag ctgggaatta
    13741 ccgttccgac gggcactaat atgcagctaa aaaacgtcgc tgcggttatg gtgacagcgt
    13801 cacttccacc gtttggacgt caggggcaaa ccattgacgt ggtggtttct tccatgggaa
    13861 atgccaaaag tctgcgtggc ggtacgttat tgatgacacc gcttaagggc gttgacagtc
    13921 aggtgtatgc gctggcgcag ggcaatattc tggttggcgg cgcaggggcc tccgctggcg
    13981 gtagcagtgt tcaggtgaat caactgaacg gtggacggat caccaatggt gcggttattg
    14041 aacgtgaatt gcccagccag tttggcgtcg ggaataccct taatttgcaa cttaacgacg
    14101 aagatttcag tatggcgcag caaatcgctg acaccatcaa ccgcgtgcgt ggatatggca
    14161 gcgccaccgc gttagatgcg cggactattc aggtgcgcgt accaagtggc aacagttccc
    14221 aggtccgttt ccttgccgat atccagaata tgcaggttaa tgtcaccccg caggacgcta
    14281 aagtagtgat taactcgcgc actggttcgg tggtgatgaa tcgcgaagtg accctcgaca
    14341 gctgcgcggt agcgcagggg aatctctcag taacagttaa tcgtcaggcc aatgtcagcc
    14401 agccagatac accgtttggt ggtgggcaga ctgtggttac tccacaaacg cagatcgatt
    14461 tacgccagag cggcggttcg ctgcaaagcg tacgttccag cgccagcctc aataacgtgg
    14521 tgcgtgcgct caatgcgctg ggcgctacgc cgatggatct gatgtccata ctgcaatcaa
    14581 tgcaaagtgc gggatgtctg cgggcaaaac tggaaatcat ctgatgatca gcgacagcaa
    14641 actactggca agtgcggcct gggatgcgca atcactcaac gaactaaagg cgaaagcggg
    14701 cgaagatcca gcggcaaata tccgtccggt ggcccgccag gtggaaggga tgttcgtgca
    14761 gatgatgttg aaaagcatgc gcgacgcttt accaaaagat ggcctgttca gcagcgagca
    14821 cactcgcctg tataccagta tgtatgacca gcagattgcc caacagatga cggcgggcaa
    14881 aggtctgggg ctggcagaga tgatggttaa acagatgacg ccagaacaac cattgccaga
    14941 ggagtccacg ccagcagcac cgataaaatt cccgctcgaa accgtggtgc gttatcaaaa
    15001 tcagacgctt tcgcagctgg tgcaaaaggc cgtaccacgt aactacgatg attcgctgcc
    15061 gggtgacagt aaagcattcc tcgcgcaact ctcgctgccc gcccaactgg caagccagca
    15121 aagtggtgtg ccacatcatt tgatcctcgc tcaggcggcg ctggaatctg gctggggaca
    15181 acggcaaatc cgccgtgaaa acggcgagcc gagctataac ctgtttggtg tcaaagcctc
    15241 tggcaactgg aaagggccag tcactgaaat caccacgact gaatatgaaa atggcgaagc
    15301 gaagaaagta aaagcgaagt ttcgggtcta cagctcgtat ctggaagcat tgtcggatta
    15361 cgttgggctg ttaacgcgta acccgcgcta cgccgccgtg acgaccgccg cgagtgcgga
    15421 gcagggggcg caggccctac aggacgcggg ctatgccacc gatcctcact atgcccgcaa
    15481 actcaccaac atgattcagc agatgaaatc gataagcgac aaggtgagca aaacctacag
    15541 catgaacatt gataatctgt tctgaataac tcaagtccgg cgggtcgctg ccgataatac
    15601 tctgtaattg aaggcttata aggaacctcc atgtccagct tgatcaataa cgccatgagc
    15661 ggactgaacg cggcccaggc ggcgttaaat acggcaagta ataatatctc cagctataac
    15721 gttgccggat atacccgcca aaccactatt atggcgcagg ccaatagcac gttgggcgct
    15781 ggcggctggg ttggcaatgg cgtctacgtt tctggtgtgc agcgtgagta tgatgcgttt
    15841 attaccaacc agttacgtgc ggcgcagacg caaagtagcg gcctgactgc ccgctatgag
    15901 cagatgtcga aaatcgacaa tatgctctcc accagtacct cttcgctggc aacacagatg
    15961 caggatttct tcaccagcct gcaaacgctg gtgagtaacg cggaagatcc ggcagcgcgc
    16021 caggcgctga ttgggaaatc agaagggttg gtgaatcagt ttaaaaccac cgatcaatat
    16081 ctgcgcgacc aggacaaaca ggtcaatatc gcgataggtg ccagtgttga tcagatcaac
    16141 aattacgcta aacaaatcgc cagcctgaac gatcagatct cccgcctgac aggcgtgggg
    16201 gcaggggcgt cacctaacaa tctgctggat caacgcgatc aactggtgag cgaattaaac
    16261 cagattgttg gcgtagaagt cagtgttcag gatggtggca cttataacat cacgatggcc
    16321 aatggctact cactggttca gggaagtacg gcgcggcaac ttgcggcagt tccttccagc
    16381 gccgaccctt ctcgtacgac tgtcgcttat gttgatggga cggcaggcaa tattgagatc
    16441 ccggagaaat tactgaatac cgggtcgctg ggcggcattc tgacattccg ttctcaggat
    16501 ctggaccaga cgcgtaatac gcttggacaa ctggcgctgg catttgccga ggctttcaac
    16561 acccaacaca aagccggatt tgatgctaac ggcgatgccg gtgaagattt ctttgctatc
    16621 ggtaagcccg cggttctgca aaacacgaaa aacaaaggtg acgttgcgat cggtgcaacg
    16681 gtaactgata cctccgcggt actggcgaca gattacaaaa tctcgttcga taataatcag
    16741 tggcaggtca cccgccttgc cagcaatacc acttttacgg tgacgccgga tgccaacggt
    16801 aaagtggcat ttgatggtct ggagttgacg tttacaggaa cgcctgccgt taacgacagc
    16861 ttcacgctga aaccggtaag tgacgccatc gtcaacatgg atgtattaat caccgacgaa
    16921 gcgaaaatcg ccatggcgag cgcagaagat gcgggtgata gcgacaaccg caacggtcag
    16981 gccctgctcg atctgcaaag caacagtaaa acggtgggcg gtgcgaaatc ctttaacgac
    17041 gcttatgcct cgttagtgag tgatatcggt aataaaaccg cgacgttgaa aaccagtagc
    17101 accactcaag gtaatgtggt gacgcagctt tccaatcagc agcagtcgat ttccggtgtc
    17161 aatctcgatg aggagtacgg aaatctgcaa cgttttcagc agtattacct cgcgaatgcg
    17221 caggttctgc agacggcaaa cgcgattttt gatgcgctga ttaacattcg ctaaggggag
    17281 ataaaatgcg tttcagtacc cagatgatgt accagcaaaa catgcgcggc atcaccaatt
    17341 ctcaggcaga atggatgaag tacggcgaac agatgtcgac gggtaagcga gtcgttaacc
    17401 cttcagatga tcccattgct gcatcacaag ctgttgttct ctcccaggca caggcgcaaa
    17461 acagccagta cacgctggcg cgtactttcg ccactcaaaa agtgtcactg gaagagagtg
    17521 tacttagcca ggtcacaacc gctatacaga atgctcagga aaaaattgtc tacgccagca
    17581 atggcacctt gagtgacgat gaccgggcct cgctggctac ggatattcag gggcttcgtg
    17641 accagttgct gaatctggcg aacaccactg acggtaacgg gcgctacatt tttgccggtt
    17701 ataaaacaga atccgcaccg tttagcgagg cggatggcaa atacgaaggc ggagcagaaa
    17761 gtattaaaca acaggtcgat gcttcgcgtt cgatggtgat aggacatacg ggcgacaaaa
    17821 ttttcgacag tattactagc aacgcggtag cggaaccaga cggtagcgct tctgaaacca
    17881 atctttttgc catgctggat agcgccatcg cagccctgaa aacgccggtc gcggatagcg
    17941 aagcggataa agaaaccgcc gctgcggcac tggataaaac caaccgcgga ctgaaaaact
    18001 cgctgaacaa tgtgctgact gttcgcgcgg aattaggcac gcaactgaac gaactggagt
    18061 cgctggattc attaggtagc gatcgcgctt tagggcaaac gcagcagatg agcgatctgg
    18121 ttgatgtgga ctggaatgca actatttcat cttacatcat gcagcaaacg gcattgcagg
    18181 catcgtataa agcatttacc gatatgcagg gattgtcgct cttccagctc aacaaataag
    18241 ctcgctttaa aacatatcat gaaactgggt atgttttgtc tgcccgctct gggatcgccg
    18301 gagcgggcat ttttttgcct attttgtatt gttggttagc aaggatgcca tttgatgaat
    18361 tttaatatgt tgactcaaag atgaaataaa aaagccctgg cagttaccag ggcttgatta
    18421 cttgagctaa ctattactca acaggttgcg gacgcgcagg agcggcagat gcatgatgcg
    18481 ttgccgtatg accacctgcg gcacctttac cttcgaaggc aaaagtaggg cgctgccagt
    18541 cactgtgacg cggtgcttcc ggaacatatt ccggtgccgg agcgcgcgtc atcggcgcgg
    18601 tagcgtggtt atgctcaacg gtgacctcag gttcaaccgc agctaccgtt tcaacttctg
    18661 ctgtgacttc agcgacgact ggcgcggcag gttgagcaac aacttcaggt tcagcaacca
    18721 caacctcagc agtttcggca acttcttcaa tatctgccgt ctcttcctgc ggttcaacca
    18781 ccggttctgc gtgttctgca acttcctggg ctacggcaac atcagactcg gtaatcacct
    18841 gcggctgttc agttaccgcg gcagcaatca cttcaggatg cgtcgtttca accacttccg
    18901 gttgcggttc ggcaacctga acgggggctt ctacgacttc agccacttct tcaacaactg
    18961 gcgcgctaac aacaggttcg gcagcggcgg cgacagggac ctcagtcacc atcggctgca
    19021 cttgtacttc ttcctgttcg cgctgctctt caacctgtac atcttgcgga cgtacaattg
    19081 gatagcggat ccagactttg ccagaggcca gttccggaga cgcgcacgct acggtcaacg
    19141 gcattggcga ctgggttgga taacgctcgt cacgatagcg acgacgacgc tgaccactta
    19201 cgcgcaggtg acgaggcgag cggcgagaac gacgcggcat gccaccgttg tcacggttat
    19261 cagcattgtt ctcttcttgc tgttctggtg cagtttgcgc tacgactggc agcggaactt
    19321 tcaccaattc tgtgcgtggg gctgccgctt cctgaacaat tggttcggct gcgacagttt
    19381 cttcaaccac cggtgcgact accgcttctt cggctacgct ttgctcgtaa cgcactttct
    19441 gattgagctg acgctgttta cgacgcggct gaaccggacg tacacgttct tcctgttcgg
    19501 tttcctgaac agattgctct tcaacattca gcgccttcac ttcttgttgc gcctgacgtt
    19561 tatcatcgtt acggcggcgg ctacgttcac gacgcggcgt ttgctgctcg tcggtggtac
    19621 gcgctttttc cgttacctct gcctgctgac ggctctcacg cgtctcggca gtctgctgct
    19681 gtgcctggcg acgattacga cggttttctt cgcgattatc gctgccttca gtacgttcac
    19741 tacgagggtc gcggcgctca ttacggtcac ggcggttgtt ctgacgaggc ttgcgacgat
    19801 cctgttgacg ttccggtttc gcttctgctt tcggtgttgg ttgctcggac ggtttggttt
    19861 cttcaccacc gctgaacagc gctttcagtg cgccgaagaa gcggctcaac agcccaggtt
    19921 gggcaggagc ggctggtgtt gccggtgcag atttaggtgc tggggctacg acaggcgcgg
    19981 caggttcagc tggcgttggt gctggcggca catccggcat ggcaaaggtt gccagcgcag
    20041 gttgttccgg acgcttacgt tcagcgaact cttcttcaga cggcagcgcc atcgcttctt
    20101 catgcagctt cggcagcatg tagcttaagg ttggggtttc ttcccctttg cgcacgcgca
    20161 gcacatggta gtgcggggtt tccatctgat cgtttggcac aatcacacag cgtacgccgt
    20221 cctgacgagt ttcaatcgca tttaccgcag aacgtttttc attcagcagg taagaagcaa
    20281 ttggcacagg aacaatggcg tgaacttcct gggtgttctc tttcagcgct tcttcttcga
    20341 tcagacgcag aatagagagc gacagcgatt cgttgtcacg aacggtaccg gtaccggagc
    20401 agcgcgggca gacgtgatgg ctggattcac ccagtgatgg gctcaggcgc tgacgggaca
    20461 tttccagcag gccaaagcga gaaatatggc tgatttgaat acgcgcccgg tcctgacgca
    20521 ccgcttcacg cagacggttt tctaccgcac gctggtggcg tactggcgtc atgtcgatga
    20581 agtcgataac aatcaggccg ccgaggtcac gcaggcgcaa ctgacgagca atctcatcgg
    20641 cagcttcgag gttggtgtta aacgcggttt cttcgatatc gccgccgcgg gtcgcgcgtg
    20701 cggagttgat gtcgatggcc gttaacgctt cggtgctgtc gataacaatg gaaccaccgg
    20761 atggcagacg aacttcacgc tggaaggcgg actcgatctg cgactcgatc tggtagtggc
    20821 tgaacagcgg gatctcgccg gtgtacagtt tgattttgct gctgaaatcc gggcggccta
    20881 atgcagcgat atgctgacgt gccagttcga gcactttcgg gttatcgata aggatttcgc
    20941 cgatgtcctg gcgtaagtaa tcgcggaatg cgcgaacgat tacgttgctc tcctgatgaa
    21001 tcaggaacgg agccgggcgg ctttcagcgg cttttttgat ggcttcccag tgtttcagac
    21061 ggaagcttaa atcccattgc agcgcctcag cagatttgcc gacgccagcg gtgcgcacga
    21121 taagccccat gccttccggc agttcaaggc ttgccagtgc ttcttttaat tcggtacggt
    21181 cgtcgccttc gatacgacga gaaataccac ccgcgcgcgg gttgttcggc atcagaacca
    21241 gatagctacc cgccagactg ataaaggtgg ttaatgccgc gcctttgttg ccgcgctctt
    21301 ctttatcgat ctgaacaatg acttcctgac cttcacgcaa cacatcttta atgttgggac
    21361 gaccatgagc actgtagtta gcagggaaat attcgcgggc aatttctttt agtgggagga
    21421 aaccgtgacg ttcagcgccg taatcaacaa aagcagcttc cagactcggt tcaatgcggg
    21481 tgattttacc tttgtagatg tttgcctttt tctgctcgtg ccctggactt tcgatatcca
    21541 ggtcatacag acgctgccca tctacaaggg caacgcgcaa ctcttcctgc tgagttgcgt
    21601 tgattaacat tcttttcatc gtaacttact cattattctt acattgacga ctaagctgcg
    21661 ggcaaagtaa cgcctttccg ggtgtgaacc gatggcctcg tgtctagtcg cgtcgccaac
    21721 ctcacggtta tcgtcagctc aaagaggcgc agagtgtcgg ttgcccgttt ttcatgcgga
    21781 aaaacagcgc aattatcaaa gaaacagact gggtattact ctccagaaat atttccatct
    21841 accggtaagg actgcaaccc gcagcccgct aactgcctga aagatcaata cgtcttacgc
    21901 cattgctgcg tggatgatcg gtcgggcaaa atgggttatt ccgtaaaatt tcttgtttta
    21961 acaaggatgg acacggaaac ggcctcatta ttccactgca agccttgtta tagcaagatg
    22021 acttttacca tttatcaccc gcttactcac agttttttca cttcttgctg gtgattggtt
    22081 taataaccat caataaagtg atcatgtagt gaagtaataa ggtgcaaaac taaatataag
    22141 catagaaaaa tgagtggcgc gaatcctgtt ggctaattag aatcgcccac catgaaaaca
    22201 gagactccat ccgtaaaaat tgttgctatc accgctgacg aagcggggca acgtatcgat
    22261 aactttttgc gtacccaatt gaaaggtgta ccaaaaagta tgatttaccg tattttgcgt
    22321 aaaggcgaag tgcgggtgaa caaaaaacgt attaagcctg aatataaact cgaagcgggt
    22381 gatgaggtgc gtatcccacc ggttcgcgtt gctgaacggg aagaagaggc ggtttcgcca
    22441 catctgcaaa aggtggcggc gctggcggac gtcatcttat atgaagatga ccatatcctg
    22501 gtgctgaata aaccttccgg tacggcggta catggcggta gtggtttaag cttcggcgtt
    22561 atcgaaggtt tgcgggcgtt gcgcccggaa gcgcggttcc ttgaactggt tcatcgtctt
    22621 gaccgggaca cctcaggtgt gttgctggta gcgaaaaaac gctcggcgtt gcgttctctg
    22681 catgagcaat tacgtgaaaa agggatgcaa aaagattacc tggcgctggt gcgcggtcag
    22741 tggcagtcgc atgtgaagag cgttcaagcg ccgttactga aaaatattct gcaaagcggc
    22801 gaacgtatcg tgcgtgtgag tcaggaaggc aaaccgtcgg aaacacgctt taaagtggaa
    22861 gagcgctatg catttgccac cctggtgcgt tgtagcccgg taacagggcg tactcatcag
    22921 atccgtgtgc atacacaata tgcgggtcat ccgattgcct ttgacgatcg ctacggtgac
    22981 cgtgaatttg acagacagct cactgaagca ggcacgggat taaatcgtct gttcctgcac
    23041 gccgcagcgt tgaagtttac ccatccgggg accggtgagg tgatgcgtat cgaagcgccg
    23101 atggatgatg gtttgaagcg ttgtttgcaa aaactgcgta acgcgcgcta atacgagcat
    23161 ataagcatga taatgggcgg ttggcctgat aaggcgttta tgccgcatca ggccgccagc
    23221 accgattgcc ggatgcgacg taaccgcatc cggcgcaaaa aactatccca tcagcgggtt
    23281 ttttccttcc cgacgtaaca tctgacacag cgcgattagc ggtaaaccaa ccagcgtgtt
    23341 aggatcacgc ccctctaaac gctcaaacag cgtaatgcca aatccttcac tcttaaagct
    23401 acccgcgcag tgcaggggat gctctttacg cacgtaatta tcaatctccg cctcgctcag
    23461 gtgacggaaa tggacgtcaa aaggctccac ttctgtttgt agatgcccat tcgccgaatt
    23521 aaacaacgcc agtccagtat agaaggtgac gatattgccg ctggctttgc gtaattgcag
    23581 acgggcattt tcttccgtta acggtttacc ggtgatttcg ccatcaagaa cacatacctg
    23641 gtctgaacca ataattaaat gatccggata acgtgacgcc agagattgcg ctttttcttg
    23701 tgccagtcga agcaccaact gtcgcggtga ttcgtcgctg cgtggagttt cgtcgacctc
    23761 gggtgctgca cattcaaaag agatttgcag tttttccaga agagcgcgac gccagggcga
    23821 ggtggaggct aaaataagtt caggcatatt tttttccatc agatatagcg tattgatgat
    23881 agccatttta aactatgcgc ttcgttttgc aggttgatgt tcgttatcag cactgaacga
    23941 aaataaagca gtaacccgca atgtgtgcga attattggca aaaggcaacc acaggctgcc
    24001 tttttctttg actctatgac gttacaaagt taatatgcgc gccctatgca aaaggtaaaa
    24061 ttacccctga ctctcgatcc ggttcgtacg gctcaaaaac gccttgatta ccagggtatc
    24121 tatactcctg atcaggttga gcgcgtcgcc gaatccgtag tcagtgtgga cagtgatgtg
    24181 gaatgctcca tgtcgttcgc tatcgataac caacgtctcg cagtgttaaa cggcgatgcg
    24241 aaggtgacgg taacgctcga gtgtcagcgt tgcgggaagc cgtttactca tcaggtctac
    24301 acaacgtatt gttttagtcc tgtgcgttca gacgaacagg ctgaagcact gccggaagcg
    24361 tatgaaccga ttgaggttaa cgaattcggt gaaatcgatc tgcttgcaat ggttgaagat
    24421 gaaatcatcc tcgccttgcc ggtagttccg gtgcatgatt ctgaacactg tgaagtgtcc
    24481 gaagcggaca tggtctttgg tgaactgcct gaagaagcgc aaaagccaaa cccatttgcc
    24541 gtattagcca gcttaaagcg taagtaattg gtgctccccg ttggatcggg gataaaccgt
    24601 aattgaggag taaggtccat ggccgtacaa cagaataaac caacccgttc caaacgtggc
    24661 atgcgtcgtt cccatgacgc gctgaccgca gtcaccagcc tgtctgtaga caaaacttct
    24721 ggtgaaaaac acctgcgtca ccacatcact gccgacggtt actaccgcgg ccgcaaggtc
    24781 atcgctaagt aattacgcgt ctgcgtgatg aagcttagtg aggatttt
//
LOCUS       DAOFSV010000056        24790 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.056, whole genome shotgun sequence.
ACCESSION   DAOFSV010000056 DAOFSV010000000
VERSION     DAOFSV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.056"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1351)
                     /gene="creD"
                     /locus_tag="QBH27_002423"
     CDS             complement(<1..1351)
                     /gene="creD"
                     /locus_tag="QBH27_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDQ7014726.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDLRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLVLLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDELRIWK
                     "
     gene            complement(1409..2833)
                     /gene="creC"
                     /locus_tag="QBH27_002424"
     CDS             complement(1409..2833)
                     /gene="creC"
                     /locus_tag="QBH27_002424"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDQ7014727.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(2833..3522)
                     /gene="creB"
                     /locus_tag="QBH27_002425"
     CDS             complement(2833..3522)
                     /gene="creB"
                     /locus_tag="QBH27_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDQ7014728.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFGVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(3535..4008)
                     /gene="creA"
                     /locus_tag="QBH27_002426"
     CDS             complement(3535..4008)
                     /gene="creA"
                     /locus_tag="QBH27_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDQ7014729.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            4219..5088
                     /gene="robA"
                     /locus_tag="QBH27_002427"
     CDS             4219..5088
                     /gene="robA"
                     /locus_tag="QBH27_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDQ7014730.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(5085..5732)
                     /gene="gpmB"
                     /locus_tag="QBH27_002428"
     CDS             complement(5085..5732)
                     /gene="gpmB"
                     /locus_tag="QBH27_002428"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDQ7014731.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            5784..6311
                     /gene="yjjX"
                     /locus_tag="QBH27_002429"
     CDS             5784..6311
                     /gene="yjjX"
                     /locus_tag="QBH27_002429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDQ7014732.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(6390..6716)
                     /gene="trpR"
                     /locus_tag="QBH27_002430"
     CDS             complement(6390..6716)
                     /gene="trpR"
                     /locus_tag="QBH27_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDQ7014733.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(6806..8743)
                     /gene="sltY"
                     /locus_tag="QBH27_002431"
     CDS             complement(6806..8743)
                     /gene="sltY"
                     /locus_tag="QBH27_002431"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDQ7014734.1"
                     /translation="MEKAKHVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            8950..10617
                     /gene="ettA"
                     /locus_tag="QBH27_002432"
     CDS             8950..10617
                     /gene="ettA"
                     /locus_tag="QBH27_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDQ7014735.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(10738..11970)
                     /gene="nadR"
                     /locus_tag="QBH27_002433"
     CDS             complement(10738..11970)
                     /gene="nadR"
                     /locus_tag="QBH27_002433"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDQ7014736.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(11991..13373)
                     /gene="radA"
                     /locus_tag="QBH27_002434"
     CDS             complement(11991..13373)
                     /gene="radA"
                     /locus_tag="QBH27_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDQ7014737.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKTPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(13422..14390)
                     /gene="serB"
                     /locus_tag="QBH27_002435"
     CDS             complement(13422..14390)
                     /gene="serB"
                     /locus_tag="QBH27_002435"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDQ7014738.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMSGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            14496..15140
                     /locus_tag="QBH27_002436"
     CDS             14496..15140
                     /locus_tag="QBH27_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDQ7014739.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            15168..16184
                     /gene="lplA"
                     /locus_tag="QBH27_002437"
     CDS             15168..16184
                     /gene="lplA"
                     /locus_tag="QBH27_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDQ7014740.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFQEQEKELRELSAWMA
                     GAVR"
     gene            complement(16185..17516)
                     /gene="yjjJ"
                     /locus_tag="QBH27_002438"
     CDS             complement(16185..17516)
                     /gene="yjjJ"
                     /locus_tag="QBH27_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HDQ7014741.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVARENRVIRFGK
                     ARATRYVLLRPYRGIERIPVWRVDDAGKAHKFADIRLCWPQGSCLVTGADGDEQWFDG
                     LPWYLTDLRPQGFLGRAWGRKLAAQLNLTDDIRLWQEEDVLYALTVFNGEYTGGWLVG
                     EGNYQRWITAQRPAEIPLDQKLTRYEQLASDALAGEIVGSSAGGEQPKFTCYAQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILRDGGIHAIESTVLVTSNRQVFLEA
                     ERFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRHLCEQQLVTHQSVAQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNIS
                     KSAWLTAIPLAQQFWQTVARDPRISEAFRHIAQEMPEKIRQIEEKVTRMGG"
     gene            complement(17683..18402)
                     /gene="deoD"
                     /locus_tag="QBH27_002439"
     CDS             complement(17683..18402)
                     /gene="deoD"
                     /locus_tag="QBH27_002439"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDQ7014742.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18459..19682)
                     /gene="deoB"
                     /locus_tag="QBH27_002440"
     CDS             complement(18459..19682)
                     /gene="deoB"
                     /locus_tag="QBH27_002440"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDQ7014743.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19734..21056)
                     /gene="deoA"
                     /locus_tag="QBH27_002441"
     CDS             complement(19734..21056)
                     /gene="deoA"
                     /locus_tag="QBH27_002441"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDQ7014744.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEHRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21134..21913)
                     /gene="deoC"
                     /locus_tag="QBH27_002442"
     CDS             complement(21134..21913)
                     /gene="deoC"
                     /locus_tag="QBH27_002442"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDQ7014745.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22171..23721
                     /locus_tag="QBH27_002443"
     CDS             22171..23721
                     /locus_tag="QBH27_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDQ7014746.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNVCECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLIDPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            23693..24556
                     /locus_tag="QBH27_002444"
     CDS             23693..24556
                     /locus_tag="QBH27_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDQ7014747.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSSGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         5790 a         6927 c         6348 g         5725 t
ORIGIN      
        1 atttccagat acgtaactca tcgtccatgg taacttcttt actggctttc attttcggca
       61 gtgaaaacgc ataccagtcg atattacggg tcacaaacat catgccggcc agcgccacca
      121 ccagcacact ggttcccaac aacagcgcgc tatcggcaga gttgagcagt ccccacatca
      181 caccatccag caacaacagc acgagggtaa acaacatgct attacgccag cctttcaata
      241 ccgcctgcaa ataaataccg ttcattaacg ccccaatcag actggcgatt atccatgcca
      301 cagtaaaacc ggtatgttca gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa
      361 gccccaccag caaatattgc attgggtgta aacgttgcgc ggtgagcgtt tcaaaaacaa
      421 agaatgccat aaaagtcagc gcaatcagca gaatggcgta cttagtcgcc cggtccgtta
      481 attggtattg atcggctggc gtcgtcactg cgacgctaaa cgcggggaag ttttcccagc
      541 cggtatcatt gcctgaagca aaacgctcac cgagattatt agcaaaccag ctactttgcc
      601 attgcgcttg aaaacctgac tcgctaactt cccgtttggc tggtagaaaa tcacctaaaa
      661 aactgggatg cggccagttg ctggttaagg tcatttcgct attacgcccg ccaggcacca
      721 cagaaagatc gccggtaccg cttaaattca gcgccacatt cagcttcagg ttctgcttgc
      781 gcaagtcccc ttcgggtaaa gggatatgca cgccctgccc gccttgctct aacccggtgc
      841 cgggttcaat ggtcagcgcc gtaccgttaa cttcaggcgc tttcaccaca ccaataccac
      901 gcgcatcccc gacgctaatc acaataaatg gcttgcctaa ggtgatattt ggcgcgtcga
      961 gttcgctaag acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg tgccagacct
     1021 gaccggtata gatgcctatc ttgcgttctt ccacattctg attgccatca accatcaatg
     1081 actcaggtaa ccaaaaatga ataaaacttc gtttccgctc cacggtttta tcgtcttcct
     1141 gcaccgtata aagctcggtc acaggaatag cgatgagcgg cccaacgagt ttttgcggcc
     1201 cgctggtact ttggcgaatc acatcttcca catcgctacg gtaatcggca cgttcgacaa
     1261 tcacctgccg aatcagcatt atcggaatca acaacagcaa tactgcacca aacaaggtgg
     1321 tcattttcca gaacaggggg gatttcaaca tagtcttctc ctttgcaatg acagcaggat
     1381 acgaaggcta tgtgggaaga atttgaagct atgtgaagtg acggtgaagt cgaagcgagg
     1441 ccagcacgcc accttcctgc acgttgcgca gcgtgacttc gccgttaaac aaacgggcga
     1501 cctcactgac aaacgccaac cccagaccgc tgcttttttg cccatttgca cgcggcaaag
     1561 agtaaaagcg ttcaaaaata cgtgacagcg cgtagtcagg aatcccacta ccggtatcca
     1621 gcaccttaag ggtgacgtat tcctgatcca cttcggcgct tagcgttatg cgaccgctct
     1681 cgggagtaaa atcgatggcg ttatccagta aattccccag cgcctgctcc agtaacgccg
     1741 gttcagcagc aacgttaatc tcagtaggca taacatgcaa agtgatgttt ttttctgcca
     1801 actgcacggt gcgcgcttcg ctgacgcggc gaaataatgc cgccacatca acagcagtca
     1861 gaacgacttc ctgacgattc tccagtcttg cctggcgtag taacgtttcc accagtgcct
     1921 gcattcgcgc attttgcgtc agaatgttgt cggtaaaacg agccaccact tccggcggcg
     1981 gaccttcgcg taaaatttcc gccgcgccac gaatcgccgc cagtgggctt tttagctcat
     2041 gagttaacgc ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac
     2101 tttccagcgc ctgcgcgagt ttacgcagct cgctactacc gagatcgggg agaggaacgg
     2161 gcttattgtc agtgacggaa tcagcatagc gagtgagccg ggcaatagag cggttgatcc
     2221 accaaaccat gcctgcgcca atcaccagtg caatccccaa caaaatggcg ctggcccata
     2281 aaattcgccg ctcgctacgc ttaatgaccg gggccatcgc cgcgttcggt ttgcctacgc
     2341 tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac atcacagaac
     2401 tttcgggatc ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta
     2461 gccagacgtc attccagcgc gaataatcct gtccaacggc tttatttgcc gaatcaaaca
     2521 atactttgcc ctgcgcatcg gtcatataga catgatattc gttgcgcact ttgttaatgc
     2581 caccgatatt ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc
     2641 catgcgttgg gtccccagag agcaaatcgg gacgcgccag ctccgccagc aacgttgcgg
     2701 tgtcgattaa cgtcccctcg gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa
     2761 tagccagtac gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc aacaaccgca
     2821 tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag tattaatcgg
     2881 tgaaagatcg gggttgatgg cgcgcagctt ggcacgcagc gttttaatgt gggtatcgac
     2941 ggtgcgatcg taggtgtcct gcgcatcttc ccacacgcta tccattagtt gctggcggga
     3001 ccagacgcgg cccggcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag
     3061 cgttaatggc gtgtcaaacc agctgatctg cgccgcaggt tcattcaatt caaaatgacc
     3121 aatacggata acgggagacg gcgtcgagaa cttcttcacc cgacgcagta aggtgcgcac
     3181 tctggcgcac acttcgcgag gtgaaaacgg tttagccacg tagtcgtcag cgccaatttc
     3241 cagcccaagc aggcgatcga cctcttcact tcgggccgtc aggaacagta caggtaacgc
     3301 cggatggagc gccagtaact ggcggcacaa ttcaaagccg ctgatatcag gcaaaccaac
     3361 atcgagaatc atgacatcgg gaacctgctg gcgagcttta tccagcactg gcaagcctcg
     3421 ctcaaagacc tcgacgccaa aaccttcctg ttgcaacatg tagaccagcg tatcggctat
     3481 cccttgctcg tcttctacta accagaccgt ttcccgttgc ataaatcgcc tctgttattg
     3541 ccgccacggc atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt
     3601 gtcggagtaa gccagataag cgagcgcgtt gcgtttagcg tcataaaagc gcacgacctg
     3661 taacgactta aagaccaggg acgtgcgttt tttgaatact acctcgccct gagctttgcc
     3721 gtttttaata cgatccgaca gttcaatcgg cccgacttgt tgacaagaaa tggccgcatc
     3781 ggaggtatct tctgccaggc ccaatccccc tttaatacca ccggttttcg cccggctcac
     3841 ataacaggtg acatttttca catcgggatc gtcaaaggct tcaacaacaa ttttgtgatc
     3901 cgggccgatc attttaaata cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg
     3961 ccccagcatt atcaggctta aagaaaggat taaatgcttg tatttcatat tgttaccatt
     4021 tttatctatt acacgagcaa ttagttcgtc acggtaaata acatattgat taagaacaaa
     4081 atctcaatac ttttatttcc gtcaagccct aaaacatact cgactaagaa aaaaacactg
     4141 aatgctaaaa cagcaaaaaa tgctattatc caattacctg atgtcaggtg ctcgttgttg
     4201 aaaggatgag gatattttat ggatcaggcc ggcattattc gcgacctttt aatctggctg
     4261 gaaggtcatc tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc aggttattcc
     4321 aagtggcact tacagagaat gtttaaagat gtcactggcc atgctattgg cgcgtatatt
     4381 cgtgctcgtc gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg tccgattctg
     4441 gacatcgcgc tgcaataccg cttcgactct caacagacat ttacccgcgc attcaagaag
     4501 cagtttgccc agactcctgc actttaccgc cgttctcctg aatggagcgc ctttggtatt
     4561 cgcccgccgc tgcgtctggg tgaattcact atgccagagc acaaatttgt caccctggaa
     4621 gatacgccgc tgattggtgt tacccagagc tactcctgtt cgctggagca aatctctgat
     4681 ttccgccatg aaatgcgcta ccagttctgg cacgattttc tcggcaacgc gccgaccatt
     4741 ccgccggtgc tctacggcct gaatgaaacg cgtccgagtc aggataaaga cgatgaacaa
     4801 gaggtattct ataccaccgc gttagcccag gatcaggcag atggctatgt actgacgggg
     4861 catccggtga tgctgcaggg cggcgaatat gtgatgttta cctatgaagg tctgggaacc
     4921 ggcgtgcagg agtttatcct gacggtatat ggaacatgca tgccaatgct caacctgacg
     4981 cgccgtaaag gtcaggatat tgagcgatac tacccggcag aagatgccaa agcgggagat
     5041 cgcccaatta atctgcgctg tgaactgctg attccgatcc gtcgttaacg ctgcagctca
     5101 tctaatgcag gggcgtccag atgcgagatg tcccctgcgg tttcaaccac ccagccagac
     5161 gccagccaca ggctttcctg ataatccacg cgcgaaatag aacagttacg cagacgtaag
     5221 cggcgctctg cccatgctgg taatccgaga atcgtactca ccaggcatcc cagtgcaata
     5281 ccatggctca ccagtagcgg tcggcttccc tgcggtaagt cccggcaaga ttccagtgcc
     5341 gcattaacgc gatcgctgag ctcctgcatt gactcgcctt caggaatacg cccgtcaacg
     5401 gtgccattga ccagttgccg acgccagttc tcttcttctt cggtgagtga atcgatattt
     5461 cttgtttcca gtacacccat atttaattca cgcaggcgag aatcaaatat gatgtcacag
     5521 ccgcaggcct gggcgatgat ttccgccgtg cgccgggtgc gtcctaaatc gctactgatg
     5581 atatgcgtaa tgccaagctc tttggcacgg gttgccacct gcatcgcctg ttgctcacct
     5641 ttggcggtca gcgggctgtc agactggccc tgaatacgtc gctcggcgtt ccactgcgtt
     5701 tcaccgtggc ggactaggta tacctgtaac atgctttttt tccgtatact gctgtaacgt
     5761 taattttctg agtaatgctg attatgcacc aagttgtctg tgcgaccacc aatcccgcta
     5821 aaattcaggc cattctgcag gcatttcacg agatcttcgg cgaaggatcc tgccatattg
     5881 catccgttgc cgtcgagagc ggtgtaccgg aacagccctt tggcagtgag gaaacgcgcg
     5941 ctggcgcacg aaatagggta gccaatgccc gccgtttact tcctgaggct gatttttggg
     6001 tggcgattga agctggcatc gatggtgaca gcactttcag ttgggtggtg attgaaaacg
     6061 ccagccagcg cggcgaggcg cgttcggcta ctttgccgtt gcctgcggtg attctggaaa
     6121 aagtgcgtga aggtgaggcg cttggtcccg tgatgtcgcg ttataccggt attgatgaga
     6181 ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaactcact cgcaccagcg
     6241 tctatcacca ggcggtgatc ctggcgctga gtccgtttca taatgccgtg tatcagccat
     6301 tgcaggcctg attaggcgtt cacgccgcat ccggcattcg gtgcacgatg cctgatgcga
     6361 cgctgacgca tcttatcagg cctacaaaat caatcgcttt tcagcaacac gtcttccagc
     6421 cactggcgca gctcgacggg cgcggctttc agactattag atccacgcgt aatcgtcgcg
     6481 atgcccgcgc cgagttcatt ttttaactca cgctggctca tttcgccgcg caacagctct
     6541 tcgacaatac gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag catcaggttt
     6601 aacaacggta aatggagatc gttttggtag gcattcttaa gcaggtcgac aaaacgtaac
     6661 cactcctggt gacgctgttc tgccatcgct gctgaatagg gtgattgttg ggccatagta
     6721 tgtcgccatt gttagcgggg gaagcaaaat gcctcccccg gttgtactcg ctaaagagta
     6781 cgatagcata tcataaacgt gtggatcagt aacgacgtcc ccattccgtg gcgctcatca
     6841 acgtcggttt atcccccatg aaatagcggt agtaagcgtc ataagccagc acgtttttca
     6901 cataaccgcg cgtctcggag aatggaatac tctcgacaaa tgccactgcg tcgatacgcc
     6961 ctgcgctgtt gccaagccag gtccgcaccc gccctggtcc ggcgttataa gctgctgagg
     7021 agaaaatacg gttattgcca aactgctgat aaacatattg caggtaactg gtgccaatgt
     7081 tgatgttcgt ttccggatcc agcaattgcc cagggctgct ataaccggga atagagaaca
     7141 tcttcaccgt atgggtcgcg gtaccaggca taatctgcat caggccgctg gcccctaccg
     7201 gtgatttcac tttcggattc caggcactct cctgacgagc aatcgccatc gcatagcttt
     7261 gcgggatctc cttaccactg gtgtagcgtt tgaaaagatc gttgtaagcc agcgggaatc
     7321 gctcttccag atgatcccac agcttcccgg cgatcgttgc ctgaacgcta agatcccacc
     7381 attggttatt gaaagcatac cgagccagtt gagcctgctc tgtttttgac ttgctcttca
     7441 ccagattggc ccactcgcta cgcgcggtgt tatcgagatt ccagtacatc aactcgcgca
     7501 cgcgcgccat ctccggcccc tgagtcaggg cgctgtcaac attctgcggc gctttatcaa
     7561 tcttcagctc atactcttcg ccgatgcgtt gtgcagcaac catcgggtag aaaccacgct
     7621 gttgcatgag ttgatgcaaa atctcttttg cttcagcttc acgtccgcgt tccagcagta
     7681 aatccgcctg ccagtaacgc cattcatctt tctctttcgc ttccataggc agacgcgcca
     7741 gccaggtatt caggccgcga cgatcgccgg tgccaagcgc cattcgcaca cggcgttcaa
     7801 taagcgaagt agattgcgag cgcataatcg cgtcatcgcg ccatttcgcc tgctcgtcgg
     7861 tgacatcgtt gcccatcaaa cgccaggcga caatatcgcg cagctcctga atctgatctt
     7921 cattaagctg ctgtgcctga gcaagcgacg ggatcatcag ccgtgcattt tcagcatcct
     7981 gccgcgccac actggcaaac gccaccgccg ccatttgacg ggtaaaatcg gtcgcgccgg
     8041 ttgtacgcgc gaaggtcagt accgtattag ggttgttcgc cagtgaaatg attgccgaag
     8101 cgatagtctg gtaatcggca ggcatctgcc ctgccagcac tgttaccagg ccggtgttac
     8161 ccgctttcat cgccagacgg atacgctcta aatacgccag cggatcttgt ttacctgacg
     8221 cacgccagac gctaaataac ttgtcacagg cgttaggctg gctcttgccg gttagccaca
     8281 gctctttcgc cccttgccag gcttcttcac tctgcccggt gttccatttc gcatagtagt
     8341 aattacattg cgcttcggta gttccgggct tttccgggct aaaggctaac aagccacgcc
     8401 agtcttcacg ccgcgccagt tcattgacga aacgagattg cagcgtgcga gcgggaggaa
     8461 gcgtggggtt agcgcgaaca aagttagtga ccgtcaccgc cggttgattc atcaggtcat
     8521 cagtgatctg gcggtattcc aggtagggat aaagtgggta atctttcagt ccaggcatca
     8581 tttgttcgac cacatccatt tgtcgattat cccaggcctg cttaatttgc gcgtaacggc
     8641 tacgctgctc atccagtgag tcggctcgcg ccacgctgct gaccgtcagc agacagacac
     8701 cggcagccaa cagccgccag gtaacgtgtt tggctttttc cacaagcgca tcctctaagt
     8761 gtaaatacat caatgcagcg tcatgccgcg taatagattt atgctaacca gtcattgccg
     8821 tttacgccac gttacggaca cttttttact tttacggggt gatcgactgg ctgggattag
     8881 acagggaaac aggctacact tcgcctttga aaactcatca tcacgataaa catagaggcg
     8941 aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg
     9001 aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa aattggtgtc
     9061 ctgggtctga acggcgcggg taagtccacc ctgctgcgca ttatggcggg cattgataaa
     9121 gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttatct gccgcaggaa
     9181 ccgcagctga acccggaaca caccgttcgt gagtccattg aagaagcggt ttctgaagtg
     9241 gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt atgccgatcc ggatgccgat
     9301 tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc tcacgacggt
     9361 cacaacctga acgttcagct ggagcgtgcg gcggatgcgc tgcgtctgcc ggactgggat
     9421 gcgaaaatcg ctaacctttc cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg
     9481 ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga tgcggaatcc
     9541 gtagcgtggc tggagcgctt cctgcacgac ttcgaaggca ccgtggtggc gattacccac
     9601 gaccgttact tcctcgataa cgttgcgggc tggatcctcg aacttgaccg cggtgaaggt
     9661 attccgtggg aaggtaacta ctcctcctgg ctggagcaga aagatcagcg tctggcgcag
     9721 gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt
     9781 cagggaacta aaggccgtca gtcaaaaggt aaagcacgtc tggcgcgctt tgaagaactc
     9841 aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc acctggaccg
     9901 cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctatgg tgatcgtctg
     9961 ctgattgact ccctgagctt ctcgatcccg aaaggagcga tcgtcgggat catcggtccg
    10021 aacggtgcgg gtaaatcgac gctgttccgt atgatctctg gtcaggaaca gccggacagc
    10081 ggcaccatca ctttgggtga aacagtgaaa ctggcgtcgg ttgatcagtt ccgtgactca
    10141 atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat catgaagatc
    10201 ggcaacaccg agatgccgag ccgcgcctac gttggccgct ttaactttaa aggggttgat
    10261 cagggtaaac gcgttggcga actctccggc ggtgagcgcg gtcgtctgca tctggcgaag
    10321 ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga cctggatatc
    10381 gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgcgc gatggttatc
    10441 tcgcacgacc gttggttcct cgaccgtatc gccacgcaca ttctggatta ccaggatgaa
    10501 ggtaaagttg agttcttcga aggtaacttt accgagtacg aagagtacaa gaaacgcacg
    10561 ctgggcgcag acgcgctgga gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc
    10621 gtaaaatgcc ggatgcggcg taaacgcctt atccggccta caaaccaagc aatttcaata
    10681 cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca ttggttctta
    10741 cctctgctcc cccatcatct cccgcaccag ctccacgcag cgcagaaaac ggctgtcgta
    10801 atcgtcctct tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagcaa
    10861 gttctggaac tctttgcgat ccaccgaact gccgaggctg cgtaaaccat ccgccaccca
    10921 cggcgtgttg ttctccagca ggatcaccag atcgaaacgg tattcatcaa tcaacgcctg
    10981 cacaaacgga tgctcacgcc cttcgtactt tttgcagaac gcctgggtag tgacaaaatc
    11041 ggtgtcgata aacgccactt tattggcata tttcactgca aaatcaatat attgcgcatg
    11101 tcccagcgcg attttatcgt agtcggaata ctgcaacgcg atctcatcgc caccgaggtg
    11161 cgaaaagaca taatcacgac catattccca cgcactggta gtgttgaaga tattggcaag
    11221 tttgtttacc agggtggatt taccgctcga ttcaccaccg aggatcgcca cggtacgcac
    11281 aaagaatggc ttcacttccg taggaatata ttcccagtag cggaacgggt tttcgcggat
    11341 ctgcgcaccg ctgatactca taaaggtacg cttcggatcg accagcaccg tctcgatccc
    11401 cagatgttcc atatactgtg gcgcatcggc ttcttccgag gtgtagatca ggtccggctg
    11461 aatccctttt tcagccataa actttttgat gccgttgctc catacatccc agccgtgtgg
    11521 atatggctcc atgccctctt cattgaaagc atgaatacgg atatttttct gatatttaaa
    11581 ggtttgcaat agccagcgca gacgatccgg caccgtgggc tgctgcgaca tggcgctgtc
    11641 ttcgaacaat gcgcggtcgc gggtatcgtc aaaacccata atgatatgca gctcgtcaac
    11701 ctggctacag gcgcgctgga taaggtagat atgtccggta tgcagcgggt aaaacttacc
    11761 gaatacgaca ccgatcgttt tcttctgccg gggaaattca agtcccaaat aacggtgcaa
    11821 tgcctccagc ttttgtgcgc tggggctttt aattttggca ttcagtaact ggcttaaata
    11881 ccctttggtc ataccgctgg catcagctac ctgctgtagc gtgcagccct gttgcttgat
    11941 ggcagttttc aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg
    12001 tcgaacacgc taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc
    12061 gtttttttcg gcacgttagc cgccggaaca atcgcccggc gaaaaccgtg tttcgccgct
    12121 tctgagattc gttcctggcc gctgggcacc gggcggatct cccctgccag cccgacttca
    12181 ccaaacacca ccagatcctg cggtagcggt ctgtcacgca ggctggaaac catcgccagc
    12241 agtaacgcta aatcggcact ggtttccgtc accttcacgc caccgaccac gttaacaaac
    12301 acatcctgat cggccatttg tagaccaccg tgacggtgaa gcaccgccag caggattgcc
    12361 agacggtttt gctccagccc gactgccacg cggcgtggat tcgccatcat cgagtgatcg
    12421 acgagcgcct gaatctccac cagcagcgga cgcgttcctt cccacactac catcactgag
    12481 ctaccggagg tcacttcatc gccgcgactt aagaaaattg ccgaagggtt gctgacttca
    12541 cgcagcccct gctcggtcat tgcaaagaca cccagctcat tcaccgcgcc gaagcggttt
    12601 ttatggctgc gtaaggtgcg aaaacgggag tcggcatcgc catccaaaag caccgaacag
    12661 tcgatacagt gttccagcac tttagggcca gccagcgagc catctttggt tacgtgccca
    12721 accatgacaa tcgccacacc gcgcgttttg gcgaagcgcg tcagataagc cgccgtttca
    12781 cgcacctgtg ccacgctgcc aggcgatgat tgtacatccg ccatatgcat cacctggatg
    12841 gagtcaatta ccatcagctt cggttgctct tcttcggcaa tcaggcagat ctgctcgatg
    12901 ctggtttccg acagcatatt aagattgtca gtcggcaggc caaggcgatg agcgcgcatt
    12961 gccacctgtt gcagcgactc ttcgccggtg acatacagcg ttttcatctg ctgcgccagt
    13021 ttgcacagcg tttgcagcaa cagcgtggat ttccccgcac cagggttacc gccaatcaga
    13081 atggcgcttc ccggcaccac gccgccgcct aatacgcggt cgaactcttt aaatccggtg
    13141 gaaaaacgcg gcagctcttc aaggctgatg tcggaaagtt tctggacttt tgccactccg
    13201 gcgctaccgg catagccact gagacgctcg ttacgcgcca ccgttggcga cgctgcaaga
    13261 cgcacctcgg tgatggtgtt ccaggcatga caggcactgc actgcccctg ccagcgcgga
    13321 taatcggccc cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacgatgtac
    13381 ctcgttaatg ctgtgccgcc cgcaggatga cgggcggaca attacttctg attcaggctg
    13441 ccagagagga tgcagaatac ccccatcagg tcagcgtgac ggatggtgac ttccgtcttt
    13501 tcattcactt ttggcttggc atggtaggca atccccagcc ctgccgcttt gatcatcggc
    13561 aggtcattgg ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc
    13621 gcgaggcggg tcagggtttt cgctttatat tgcgcgtcaa cgatgtcgcc gatcacattg
    13681 ccggtaaatt taccgtccat gatctccagt tcattggcta ccacggctgt caggcgcagc
    13741 ttgtcgcgca ggtattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag
    13801 cccagcgttt ccagcttgag taccagctgc gttaagcctg acatcagcgg cagattttca
    13861 cgcacctgtt gcagaatatt ggcgtcagcg cctttcagcg tcgccacgcg gctgcgcagg
    13921 ctggcggtaa aatcgagttc gccgcgcatc gcccgttcgg ttacctccgc caccctctcg
    13981 cccgttccgg ccagtttggc aatttcatca atacattcaa tctggatggc ggtggaatcc
    14041 atgtccatca ccagcaaacc cggcgtgcgc aggtgtggga ttttccccag cggtgcgaca
    14101 tccagttgcg cttcatgggc caggcgcgta gcccgcgcag tgagtgaacc tgccagacga
    14161 atcacctgat aatcttccac gcaccaggcg gcaacaatca ccatagccgc gcccagtttg
    14221 ctctggtatt gggtcagacg ttgtttatcc agcccacgac catacagcag ccagccgcta
    14281 cgacctgcgt ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagacca
    14341 ggccataaag agacatcttc aggcaagtcg caccaggtaa tgttaggcat taaggctcct
    14401 gtaaaatcgt tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg
    14461 gcaacattaa gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc
    14521 ggctgcatcg ggcagtgatt gtcctgttct gtcttgcctt gttagtggcg ctgatgcagg
    14581 gggcgtcatg gtttagtcaa aaccaccagc gacagcgtaa tccgcaactg gaagaactgg
    14641 cccgcaccct ggcgcggcag gtgacgctaa acgttgcacc gctgatgcgt accgactcac
    14701 cggatgaaaa acgcattcag gcgatcctcg atcagttaac ggatgaaagc cgcattctcg
    14761 acgcaggcgt atatgatgaa caaggcgatc ttatcgcccg ttctggcgaa agcgtcgacg
    14821 tgcgcgaccg gctggcgctc gacggcaaaa aagcgggcgg ctattttaat cagcagattg
    14881 tcgaacccat tgcgggtaaa aacggaccgc tcggctatct gcgcctgacg ctcgacaccc
    14941 atacgctcgc caccgaagcc caacaggtgg ataacaccac taacatttta cgcctgatgt
    15001 tgctgctctc gctggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgca
    15061 cccgctggca gcaatcgccc ttcctgttaa ccgccagcaa accggtgccg gaagaggaag
    15121 aaagcgagaa aaaagagtga cccattacta caagaaagga aatcgttatg tccacattac
    15181 gcctgctcat ctctgactct tacgacccgt ggtttaacct ggcggtagag gagtgtattt
    15241 ttcgccagat gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag
    15301 tgattggtcg tgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata
    15361 acgtccgcct ggcgcggcgc agtagcggtg gcggcgcggt gttccacgat ctcggcaata
    15421 cctgttttac ctttatggct ggcaagccgg agtacgataa aactatctcc acgtcgattg
    15481 tgctcaatgc gctgaacgcg cttggcgtca gcgccgaagc ttccgggcgt aacgatctgg
    15541 tggtgaaaac cgccgaaggc gaccgcaaag tctcaggctc ggcctatcgc gaaaccaaag
    15601 atcgtggctt ccaccacggc actttgctgc tcaatgccga ccttagccgc ctggcaaact
    15661 atctcaatcc ggataaaaag aaactggcgg cgaaaggcat tacctcagtg cgttcccgcg
    15721 tgaccaatct gaccgaattg ctaccgggga tcacccatga gcaggtttgc gaggccataa
    15781 ccgaggcctt tttcgcccat tatggcgagc gcgtggaagc ggaaatcatc tccccggaca
    15841 aaacgccaga cttgccaaac ttcgccgaaa cctttgcccg ccagagtagc tgggaatgga
    15901 acttcggtca ggctccggca ttctcgcatc tgctggatga acgctttacc tggggcggcg
    15961 tggaactgca tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg tttaccgaca
    16021 gtctcaaccc cgcgccgctg gaagccctcg ccgggcgact gcaaggctgt ctgtaccgcg
    16081 cggatatgct gcaacaggag tgcgaagcgc tgctggttga cttccaggaa caggaaaaag
    16141 agctacggga gttgtcggca tggatggcag gggcggtgcg gtagttaccc acccatgcgg
    16201 gtaactttct cttcgatttg ccggattttt tccggcattt cttgcgcaat gtggcgaaac
    16261 gcctcgctga tacgcggatc tctggcgacc gtttgccaga actgctgcgc cagcgggatc
    16321 gccgttaacc atgcgctttt actgatgtta agatcaaact tcacctcaat ggcagcatca
    16381 cgcagcattc cggcgctgtt tggcgcatag gccatcggca gcatgtcgta gacgggtgtc
    16441 agcgcaaatg gtggttcaga taaataaaat gataaattgc ctgcgtgcat atcgctgttg
    16501 gcgataagtc gcccaaacgc ccagatcact tctgtttgcg ccacgctctg gtgagtgaca
    16561 agttgttgct cacacaaatg ccgcattgcc tccggccacg atcccggaga agagataaac
    16621 tcactttgca ccgcctccag cgacacaatg ggcaagcgac catcgttacc tttgcagtca
    16681 aagcgttccg cttccaggaa tacctgcctg ttacttgtta caagcaccgt tgactcgatg
    16741 gcgtggatcc cgccgtcacg caggatttgc gcggcaatag attcagcaat tagcaggtcc
    16801 ccccaacgtt ggctgactgc ggtttgctgt ggcacggtga atttcaccaa cacatgttta
    16861 ttgcctgacg gcgtttgtgc atagcaggta aattttggct gctcgccgcc cgcagaagaa
    16921 cccacaattt ctccagccag agcatcgctt gccaactgtt cgtaacgggt gagtttttga
    16981 tccaaaggaa tttccgcagg gcgttgtgca gtaatccatc gctgataatt cccctccccg
    17041 accaaccaac cgccagtata ttcaccgtta aatacggtca gggcgtagag cacatcttct
    17101 tcctgccaga gacgtatatc atcagtcaga ttcagttgcg cggctaactt tctgccccac
    17161 gcgcgcccta aaaagccctg cggtcggaga tcggtcaaat accagggcaa accatcaaac
    17221 cactgttcgt cgccatctgc gcctgttacc agacaacttc cctgtggcca gcacaaccgg
    17281 atgtcggcga atttatgcgc ctttccggca tcgtccaccc gccagacggg aatacgctca
    17341 attccgcgat aaggacgcag cagtacatat cgcgttgccc gtgctttacc aaagcgaatc
    17401 acccgatttt ctctggcaac aaggcgtgag aacgtcgcct gactgattgc cagacgctgg
    17461 cgcaattccg gggcagaacg cggcccttgc agtaaaagat cggtcagttc gctcattaat
    17521 gaatagattc gtgaatagat acttgaatag atttattaac atatttcact gaaaagtata
    17581 gcgaaaatat tcaagctgga gagataaatg aataaatact aaaaagccgg agcagtctcc
    17641 cactccggct tcacacgaca atcgcctttc agcaaaacac aattactctt tatcgcccag
    17701 cagaacggat tccagcgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc
    17761 agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc
    17821 gccaaattct gcagcgacgc cgtagatacc cgccgcttcc atttccacgc cgaggatgcc
    17881 gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa
    17941 caggttacca acgcgagcat caacgcccag tgcttttgcc gcgtctaccg cgttacgcac
    18001 catgtcgaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt
    18061 ggaatcggtg caggcaccca taccgataac gacgtcgcgc agttttacgt gcggcagaac
    18121 agcgccacag gaacccacgc ggataatttt cttcacgccg aaatcagtga tcagttcttt
    18181 ggtgtagatg gagcaggacg ggatacccat accgtggccc attacggaaa ttttgcggcc
    18241 tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc
    18301 aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc
    18361 agcaaaatcg cccatttctg cattaatgtg tggagtagcc atcgttttat cctttttgtg
    18421 acatattaaa ggcggagtca ctgctccgcc caaatccatc agaacatggc tttgccatat
    18481 tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca
    18541 cgatgaccca gcgaacctgg tttcactttc gggccgtaaa ccagtaccgg aatgtgttca
    18601 cgcgtgtggt cagtaccggt ccaggtcggg tcacaaccgt ggtcagcagt gagaatcaga
    18661 atatcgtcat cgcgcagcaa agacatcagc tccggcagac ggcggtcgaa cagttccaga
    18721 cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa gtcaacgaag
    18781 ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac
    18841 agcgcgtcca gcccggtcgc tttcactttt ttggtgatac cgcagttggc atagatgtct
    18901 gcaattttac cgaccgaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg
    18961 gtcggtgctg gcggctcaac agccaggtcg tgacggttac cagtacgctg gaagttaccg
    19021 gctttgtcgc cgataaacgg acgagcgata acacgaccga tgttgtagcc gccgttggtc
    19081 agctcttcac gggcgatttc gcacagctcg tagagtttat ccaggccaaa ggtttcttca
    19141 tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc
    19201 atatgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg
    19261 taacccggca gattagcgcg ttcgaccagt ttatccagca actcttgcgg gaagctgttt
    19321 tcgtgatcgg agaagtatcc ccactcaaac agaaccggca caccagcaat ttcccagtga
    19381 ccagacgggg tgtctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact
    19441 tcagcgttgc catccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc
    19501 agcccaagac gggtcagatt tggcagattg agcgggcctt tacgaccgtg gtcagcttcg
    19561 cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt
    19621 tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc
    19681 atatgttctc cgtactcagt gcttcaaatt taaatgatcg gatcagtata ccgttattcg
    19741 ctgatgcggc gatagacagt tggtgtgctt tccggtgctt tatcggcaag tttaattgcc
    19801 gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgtg gataactgcc
    19861 agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa gccgacgctg
    19921 taatcgatgg tgtcagatgc ctgacggcgt ccgccgccca ttgcaaccac tgccatcccc
    19981 agtgcgcggg tatccatttc actgacaaac ccttcggtat cagcatagac tgctttcgtc
    20041 agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt
    20101 gccgcgacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat
    20161 ttcgcgcgcg cttcggcgtc atttttcgcc agtttgccgg agatcagcat ctccacgcac
    20221 agtgccatcg tgacatcaaa cagacgcggg ttacgatgtt cacccgtcag gaactgcacc
    20281 gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt catatcggtg
    20341 agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg
    20401 gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc
    20461 agcgcgtcca gaccttccgc cagtttcttc gccagaatag aggcggtgat cagcgggatt
    20521 gaatccacgg ttgcggtaat atcgcgggtc gcgtagaaac gtttatccgc cggagccagt
    20581 gagctggtct ggccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg
    20641 tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta
    20701 tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cacaggcagc gaccattggc
    20761 cccaacatca gcgaagtcac atcgccgaca ccgccggtcg agtgtttatc aacaatcggg
    20821 ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc
    20881 agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca
    20941 atctgccctt cggagatagt gttgtcgcga atgccgttga taaagaaacg aatttcttca
    21001 tcactcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta
    21061 acctcccgtg gaaaagagac gaggtaatcg gggaaccgga tgagcatcgc tgcatcatcc
    21121 ggcaaatcaa gccttagtag ctgctggcgc tcttaccgtc gccgtggccc agagctttca
    21181 gcaggcttgc cagcaagctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag
    21241 caccgaacag ttcatctgca atggcgagat atttctgcgc atcttccgca gtacgcacgc
    21301 cgcctgccgg tttgaaacca acggtttttt ctacgcccat atcgcggatc acttccatca
    21361 tgatgcgcgc gctttccggc gtcgcattca cagccacttt accggtagag gttttgatga
    21421 agtcagcacc cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca
    21481 gttcgccggt ttcaataatc actttcagca gtacgttcgc cgcagcgcaa gcctctttac
    21541 aggctttcac caggtcaaaa ccaacctgct cgttacctgc catcagcgcg cggtacggga
    21601 acaccacgtc aacttcatcg gcaccgtagg cgattgccgc acgggtttct gccagcgcga
    21661 tttcgatgtc gtcgttaccg tgtgggaagt tagttaccgt agcgatacgg atttccgggg
    21721 tgccctgctc tttcagagtt ttgcgagcaa tcgggataaa gcgaggatag atacagatag
    21781 cggcggtatt gccgaccgga gttttggcct gatgacacag tgcgatcact ttctcatcgg
    21841 tgtcgtcgtc attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt
    21901 tcagatcagt catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt
    21961 tagtattcta acatctactc cgcaacacac ttcgatgtac atcacaatta aggaaatcta
    22021 cttacaagtt tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg
    22081 cgtcagtggt ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc
    22141 gcatcacctt cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa
    22201 caacgttgcc agcaaattgt caccagcccg gtacttagcc cggagcagaa gcgccatttt
    22261 ctggcactgg aagcagaaaa caatctgcct tacccacagc tgcctgccga agcccgccgc
    22321 gcgctggatg agggggtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc
    22381 tatgtcttac ccgattacgc tcgttttctg gcgcacggtt ccgaatggct ggagctggaa
    22441 ggcgcgaaag accttgatga cgcactctct ctgctgacca ttctttacca ccatgtaccg
    22501 tcggtcacat cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt
    22561 agaattctaa cacaagacga gatcgatatt cgaataaaac gtttctggcg ttacctcgac
    22621 agaaccctgc cagacgcctt tatgcatgcc aatatcggtc cgtctgattc gcccattacc
    22681 cgtgcaatat tacgtgcaga cgcagagctg aagcaggttt caccgaacct gacctttatc
    22741 tacgatcctg atatcacccc tgatgacctg ctgctggaag tggcgaaaaa cgtctgtgaa
    22801 tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc
    22861 tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta
    22921 cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc
    22981 actctaccgc actactgcca acagcagatc gccatcatcg atgcgcggtg tgaattcctc
    23041 tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggtt gattgatcct
    23101 gaacgttttg tgccgatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt
    23161 gaaaaagaag ggattgccgc acgctacggt aaagaggccg ccgcaaatga agtgggttat
    23221 cgcattagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa
    23281 aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc
    23341 gcgcgtttgc catatggtga tgagccagat ccgattaccc atctgcaaac tgtcgccccg
    23401 catcatgctt attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa
    23461 cgtaatccgc aggcgctggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa
    23521 tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg
    23581 tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggagaa
    23641 gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag
    23701 cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg
    23761 tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac
    23821 gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca
    23881 gattgttgac ggcaaagtgg tgtggaacgc tgtggtttgc gagcagtgtg atacctgcct
    23941 gaagaggtgt ccgcaacatg ccacgccaat ggcgcaatcc atgagcgtgg acgaagtgct
    24001 tagccatgtc cgcaaagcgg tgctgtttat tgaagggata acggtaagca gcggcgaagc
    24061 cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact
    24121 gcgccacctc acctgcctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa
    24181 attactcccg gtgtgcgacg gcgcaatgct cgatctcaaa gcgtggggga gcgaatgtca
    24241 tcagcaatta accggacgcg ataatcagca gattaagcgc agcatctgtt tgctggcaga
    24301 gcgcggtaag ctggcggaac tgcgtttgct ggtgatccct ggccaggtgg attatttgca
    24361 acacatcgaa gaactggcgg cgtttatcaa gggactcggc gatgttccgg tacgcctgaa
    24421 cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga
    24481 agacgttgag ccgttggctg atgcgttaaa ggtgcgcggg gtgagccggt tgatatttcc
    24541 ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcatg
    24601 ataagacgcg acaagcgtcg catcaggcat tatgcgccga ctgccggatg cggcgtgaac
    24661 gccttatccg gcctacggtt cggcacagac ttgtaggcat gataagacgc gtcagcgtcg
    24721 catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt
    24781 cggcacagac
//
LOCUS       DAOFSV010000057        24579 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.057, whole genome shotgun sequence.
ACCESSION   DAOFSV010000057 DAOFSV010000000
VERSION     DAOFSV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.057"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(38..1000)
                     /gene="ldtC"
                     /locus_tag="QBH27_002445"
     CDS             complement(38..1000)
                     /gene="ldtC"
                     /locus_tag="QBH27_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDQ7014748.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1143..4589)
                     /gene="mfd"
                     /locus_tag="QBH27_002446"
     CDS             complement(1143..4589)
                     /gene="mfd"
                     /locus_tag="QBH27_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDQ7014749.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPSMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGIDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFMAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(4716..5789)
                     /locus_tag="QBH27_002447"
     CDS             complement(4716..5789)
                     /locus_tag="QBH27_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDQ7014750.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NPLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            6050..7249
                     /gene="lolC"
                     /locus_tag="QBH27_002448"
     CDS             6050..7249
                     /gene="lolC"
                     /locus_tag="QBH27_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDQ7014751.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7242..7943
                     /gene="lolD"
                     /locus_tag="QBH27_002449"
     CDS             7242..7943
                     /gene="lolD"
                     /locus_tag="QBH27_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDQ7014752.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            7943..9187
                     /gene="lolE"
                     /locus_tag="QBH27_002450"
     CDS             7943..9187
                     /gene="lolE"
                     /locus_tag="QBH27_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDQ7014753.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQDDAWSNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9216..10127
                     /gene="nagK"
                     /locus_tag="QBH27_002451"
     CDS             9216..10127
                     /gene="nagK"
                     /locus_tag="QBH27_002451"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDQ7014754.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10143..10964
                     /gene="cobB"
                     /locus_tag="QBH27_002452"
     CDS             10143..10964
                     /gene="cobB"
                     /locus_tag="QBH27_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDQ7014755.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11102..11890)
                     /locus_tag="QBH27_002453"
     CDS             complement(11102..11890)
                     /locus_tag="QBH27_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDQ7014756.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(11887..12348)
                     /locus_tag="QBH27_002454"
     CDS             complement(11887..12348)
                     /locus_tag="QBH27_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDQ7014757.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEGSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12406..13452)
                     /gene="potD"
                     /locus_tag="QBH27_002455"
     CDS             complement(12406..13452)
                     /gene="potD"
                     /locus_tag="QBH27_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDQ7014758.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13449..14243)
                     /gene="potC"
                     /locus_tag="QBH27_002456"
     CDS             complement(13449..14243)
                     /gene="potC"
                     /locus_tag="QBH27_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDQ7014759.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14240..15097)
                     /gene="potB"
                     /locus_tag="QBH27_002457"
     CDS             complement(14240..15097)
                     /gene="potB"
                     /locus_tag="QBH27_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDQ7014760.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFIFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15081..16217)
                     /gene="potA"
                     /locus_tag="QBH27_002458"
     CDS             complement(15081..16217)
                     /gene="potA"
                     /locus_tag="QBH27_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDQ7014761.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16467..17693
                     /gene="pepT"
                     /locus_tag="QBH27_002459"
     CDS             16467..17693
                     /gene="pepT"
                     /locus_tag="QBH27_002459"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDQ7014762.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(17742..18863)
                     /gene="roxA"
                     /locus_tag="QBH27_002460"
                     /gene_synonym="ycfD"
     CDS             complement(17742..18863)
                     /gene="roxA"
                     /locus_tag="QBH27_002460"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDQ7014763.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18939..20399)
                     /gene="phoQ"
                     /locus_tag="QBH27_002461"
     CDS             complement(18939..20399)
                     /gene="phoQ"
                     /locus_tag="QBH27_002461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDQ7014764.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20399..21070)
                     /gene="phoP"
                     /locus_tag="QBH27_002462"
     CDS             complement(20399..21070)
                     /gene="phoP"
                     /locus_tag="QBH27_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDQ7014765.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21239..22609)
                     /gene="purB"
                     /locus_tag="QBH27_002463"
     CDS             complement(21239..22609)
                     /gene="purB"
                     /locus_tag="QBH27_002463"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDQ7014766.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNHDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(22613..23254)
                     /gene="hflD"
                     /locus_tag="QBH27_002464"
     CDS             complement(22613..23254)
                     /gene="hflD"
                     /locus_tag="QBH27_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDQ7014767.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23290..24396)
                     /gene="mnmA"
                     /locus_tag="QBH27_002465"
     CDS             complement(23290..24396)
                     /gene="mnmA"
                     /locus_tag="QBH27_002465"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDQ7014768.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(24450..>24579)
                     /gene="nudJ"
                     /locus_tag="QBH27_002466"
     CDS             complement(24450..>24579)
                     /gene="nudJ"
                     /locus_tag="QBH27_002466"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDQ7014769.1"
                     /translation="ILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
BASE COUNT         5946 a         6254 c         6412 g         5967 t
ORIGIN      
        1 cgacatgccg tgtcgcgggg atttttttat ccgagcttta cagagtttgt gggctcactt
       61 gatgacggcg gacatccacc ggcatcccgg aacggacatc catcacatgt tgcatcactt
      121 cagcgtcagt ttgtgctgca tctttaaatg attgcattgc gctattcagc gtaattggca
      181 gcagctgcgg atcgtcatca atcttctctg acagcggctg atgtacttca accagacgcg
      241 caccgtttgg ttcggcagag actttaatcg gggtgttgat gatattcact ttggtgcctg
      301 gggtgacctg gctaaagagt gttttgatat cgtcatcccg cagacgaata cagccagaac
      361 ttacccgcat gccaatgccg aaatcagcgt tcgtaccgtg aagcaaataa acgccgccat
      421 aggccgccag acgaatcgca tgatggccca ttgggttatc cggtccagcc ggaactaccg
      481 caggcaactc aattccctgt gccttatacc gcgcgcgaat gtttgccgtt ggcgtccagg
      541 ttgggtttgc acgcttgtca gaaacggtgg tcaccattgt cggtgtcagc gtgtcaccac
      601 ctaattgacc aatgccgatg ggatatacgg ttaccgaatt ttttcccggc ggataataat
      661 agagacgcag ctccgcaatg ttgatcacaa tgccttcgcg cggcgcgtct ggaagtaggg
      721 tttgcaacgg gatcgttaac acgctacccg cgcgcggtac gtaaggatca acgccggggt
      781 tagcctgtaa cagagcgaga aagccgacgt tatatttttt ggcgatggct tccagagaac
      841 caccgtcatt ttccacaaca tgaaatttgt tttcaccaac cagacgactg cctgctgacg
      901 gcagcggcca ggtgttggct tttgccggta gcgccagcgc cacggcagcg gcgaacgtaa
      961 aaaatgttag ccagcgagaa aaacgcgttt tgatcatcac caaagtccat aataaatata
     1021 aggttattgt aataaaaagg taagtctgaa ttatggaggg tgacagtgtc ggataatgca
     1081 gggaagtgca aagaatttgt aaatattgca gatgggggcg cagaaacgcc cccgatttac
     1141 cattaagcga tcgcgttctc ttccagttca cgcataaact ggcgtaccca ttcgatacgc
     1201 gttttccgct cgctcaaatc ctgaataaac ttcagacgcg tcggaccatc aaggcggtaa
     1261 tgctgcggct gtttttgcag caaaccaatc aaccaggccg gattaacgtg attcttctcg
     1321 gcaaattcga tcacgccgcc tttctcatta ccttccagct tcctgatccc cagtttctgc
     1381 gcttgctggc gcagacgggc aatatccagc agggtacgcg ccggatccgg cagcaggccg
     1441 aagcgatcga taagctcgac tttgatctct tccagttcgt tttccgtttt ggcgctggca
     1501 atacgtttgt agaacgacag acgcgtgttc acgtcaggga tgaaatcatc tggcaatagc
     1561 gacggcatcc gcagctcgac ttctgtttgc tggctggtga gatcttccag cgacggctcg
     1621 cgtccggctt tcagcgcatc gacggcgttt tccagcaact ccatatacag cgagaaaccg
     1681 atggtttcca ttgagccgct ttgttcttcg ccaagcagtt cacccgcgcc gcggatctcc
     1741 agatcgtgcg ttgccagcgc aaaacctgca ccgagatctt ccagcgaggc aatcgcttca
     1801 agacgttttt gcgcatcggt agtcatcgct tttggatgcg gcgtcagcag ccatgcatat
     1861 gcctggtggt gcgaacgccc gacgcgaccg cgcaactggt gcaactgtgc cagaccgaag
     1921 tgatccgcgc gttcaatgat aatggtgttg gcggtaggaa tgtcgatccc ggtttcgata
     1981 atggtggtac aaaccagcac gttgaaacgt tgatgatgga aatcattcat cacccgttcc
     2041 agttcgcgct cacgcatttg cccgtgaccg atagcaatcc gcgcttcagg taccagttct
     2101 gccagccgtt cggcggcttt ctggatgttt tccacatcat tgtagagata ataaacctgt
     2161 cctccgcgca aaatttcacg caggatcgcc tcccggacca ccagactgtc atactcacgg
     2221 acaaaggttt taactgccag acgacgggcg ggcggcgtgg cgataatcga cagatcgcgc
     2281 attccgctca ttgccatatt cagcgtacgt gggatcggcg ttgcggtaag cgtcagaata
     2341 tccacgttcg cgcgcatcgc tttaatgcgc tctttatgac gcaccccgaa gcggtgttct
     2401 tcatcgacaa tcagcaggcc taaatcttta aacttgacgt cactttgcag cagtttgtgc
     2461 gtaccgatca gaatatcaat tttcccttcc gccacttccg caaggatttg cgtctgctct
     2521 ttggcgctgc ggaaacggga gatcatttcg atacgtaccg gccagttggc gaaacggtcg
     2581 cggaagttgt cgtaatgctg ctgcgcgaga agggtggtag gcaccagcac cgccacctgt
     2641 ttgtggttat ctaccgccat gaaagcggcg cgcatcgcca cttctgtttt accaaagcca
     2701 acatcgccgc acaccagacg atccattgcc agcggctgac acatgtcact aagcaccgca
     2761 ttaatggcct gcgcctgatc cggcgtggtt tcaaacggga agctgtcgca gaacaactga
     2821 tactgctcgc gatcgtgttt aaacgcgaag ccctctttgg cggcgcgttg cgcgtagata
     2881 tccagcaatt ccgccgccac atcacgcact ttttccgccg ctttctgccg cgcgcgtgac
     2941 caggcatcgc caccaagttt atgcagtggt gcgttttctt ctgcgccacc cgcgtaacgg
     3001 ctaatcagat gcagtgacga caccggaaca tacagtttgg cgtcgttggc ataggtgagc
     3061 atcaaatact cgccagtaat gcctccagct tcgagcgtgg tcattccggc ataacgaccg
     3121 acgccgtgct ccagatggac caccggctga ccaatatgca gctccgcaag gttacggatc
     3181 agtgtatcgg ggttgatggc gcggcgagaa tcctgacgac gacgggcaac gcgttcaccg
     3241 agcagatcgc tttcgcaaat cagcgccaga ttacgtaccg tatcgacaaa accatgctct
     3301 gcagcgccaa tcatcagata acgcccacgg tcgctggctt catcaagacg cataatgcgt
     3361 tgcggagcaa ttttaattcg cgcgagcagt tcacccaacg cttcacggcg gccttcactt
     3421 tctaccgaga acaccaccgg accgtcgaaa gactcgagga acttacgcag cgcatccagc
     3481 ggcgcttttt gttgtgcctg aatggccagg tctggcagtt tctggaaacc taaattggca
     3541 ttcgctgctt ttgtcggtaa atgttcagtt tttagctgca cacggggcca gtttttcagc
     3601 tctgagaaga gctcatccac ccgcagccag agcgattgtg gtggcaacag tgggcgcatt
     3661 gggtcgatgc cgcgattctc aaaacgcgcg agcgtgtcag cctggaaacg ttcggcactg
     3721 ttttccagat cgccagtatt caccaacagc gtattggcag ggaaataact gaacagtggc
     3781 ggcagcggtt cgctgaagaa tagcggctgc cagtactcga tcccggcagg taatgtgcct
     3841 ttactcactt gctgataaat atgttccggg tcgcgcttca cttcgaaggt atcacgccac
     3901 tggctgcgga acagttcaat tgccgcttta tcggtcggaa attcgtgcgc gggcaacaga
     3961 ttgatcgctt ctacttcctc cagcgtgcgc tggctatcga cgtcaaacac ccgcaggctg
     4021 tcgatttcat catcaaagaa atcaagacga taaggcagct cactccccat cgggaagaga
     4081 tccaacaacg cgccgcgcgt ggcgtattcg ccgtgctcca tcacctggtc aacatggcga
     4141 taaccggcgc tgtccagttg ggttcgtaat gcatctcgtg acaggcgctg accttttttc
     4201 atcaccagcg catgaccgtg gagaaagctg tgcgggcaaa cgcgctgcat aagcgtattc
     4261 accggaacaa tcagtacgcc acgctgcatc gatggtaact ggtaaagggt ggaaagacgc
     4321 gaggagataa tgtcctgatg aggcgaaaaa ctgtcgtaag ggagggtttc ccagtccgcc
     4381 agattcatca ccatctgatc ggtgaactgg ctgatttcat catgcaaacg cagagcattt
     4441 tgcatatctg gtgcaatgag taccaccgga ccggcgtgac gttcggcaat ttctgctacc
     4501 agcgtcgcgc aggcagcgcc ggttaactcg cccagcagac gctgctcacc cgctttgacg
     4561 ggcagcgtat aacgatattg ttcaggcata acggttgtca gattctcgtt aggatatgcc
     4621 tcaacatatg gggcatatct ctgataagca atgctgttca ttatccgtaa tcgacatggt
     4681 ttagcaaatt gaatcgcccc ggctggggcg atggcttagc gggagtaact gacggtagcg
     4741 ggcagagaag gcggggagaa caacagatcg ctgaatgcgc gctgtgaaag ttttcttacc
     4801 agcaagcccg caacagtaca aatcaacaaa ctgacaaagg ggtaaatcag caggagcgtc
     4861 agttcgactt gcggcgacca gcgtaccgcg ttcatttgcg gaatcaggct taagctgaat
     4921 atttctacca gaatgcgatg ggtggtatag atagcaatgg tgttggagcc aatcacattc
     4981 agcaggctgg aggaacgcat accgaaacgc tgctcgtact gataaaacag cttcatgatt
     5041 accacaatcg ataccagcga caacagcagc gagatattaa acaaccaggc accgaccgcc
     5101 agaacggtca gcaaagaagc catcagcaga tggcggcgca acggtaccgc tttaacacag
     5161 gtcattaatg ttgcgccaaa ccatgcgcca aggctgtaat aaggcaaatt gcggatcaca
     5221 ctgttcattc cccaccacgg tgtgggtacg aaattaacag ccacactcat cagtacaaac
     5281 agaacaaata acggcagggc cagggggtta aaaattttac atatcacgaa atagacaatt
     5341 aacgcataca gataccacaa gctggtgctg gcggtgatca tcccgtgcag gaactcaccg
     5401 gtggaatcgg cataagcggc attggaggca ttgcttaaat cgcgctcagg tgccagccac
     5461 tggttcagcg cacttaacgc cagccactgc accacgcccc aaagcgccag cacccagaag
     5521 atgttccaga tgcgtttatc gagacaattt ccccacggta cgctgtcgat atagcggcga
     5581 atcaaatagc cagagataaa gaaaaaaacc ggcatacgaa agggtgcaag gtaaagattg
     5641 aaatagatcc agcatttgct caggacttcc gataacggat gctgaaaagt ggtcagatgc
     5701 ggataaaagg taatgaccga gtgataaatc accaccagac aaatacataa ccctttgatc
     5761 tggttaatcc atagctcttt ttgtttcatc gggacaacac gctcttttta tttaacatgc
     5821 ggttgatcct gcggggcgag tcgcgtggtg taatgcgtat ctgtctgtat ttggttattt
     5881 ttcagataaa gatcaggcca catggccgat gataaaatga ggcactttag ccgattgatt
     5941 tgctggtact tttcagatta gccctgacga tcacttacag ttcagacgtt tacccatcct
     6001 gctttcgctt atatactcgt gtctttgcta cagcaaccag acggatttca tgtaccaacc
     6061 tgtcgctcta tttattggcc tgcgttacat gcgtgggcgt gcagcggatc gcttcggtcg
     6121 tttcgtctcc tggctttcta ccatcggcat taccctcggg gtgatggcgc tggtcacagt
     6181 attgtcagtg atgaacggct ttgagcgcga gctgcaaaac aacatccttg gcctgatgcc
     6241 acaggcaatt ctctcttctg agcatggctc tcttaacccg cagcaactcc cggaaacggc
     6301 agtcaaactg gacggcgtta atcgcgtcgc acctattact accggtgatg tggtactgca
     6361 aagcgcgcgc agcgtggcgg tcggggtgat gctgggtatc gacccggcgc aaaaagatcc
     6421 actaacgccg tatctggtca atgtgaaaca aactgacctc gagccgggga aatataatgt
     6481 catcctcggt gaacagcttg cctcacagtt gggcgtcaat cgcggtgatc aaatccgcgt
     6541 gatggtgcca tctgctagcc agttcacgcc gatggggcgt attccaagtc agcgcctgtt
     6601 caatgtgatt ggcactttcg ccgccaacag tgaagtcgat ggctatgaaa tgctggtgaa
     6661 tattgaggat gcctcgcgtc tgatgcgtta tccggcaggc aatattaccg gctggcgttt
     6721 gtggctggat gagccgctga aagtcgactc attaagtcag caaaaactgc ctgaaggcag
     6781 caaatggcag gactggcgtg accgtaaagg cgagctgttc caggccgtac gcatggaaaa
     6841 aaatatgatg ggcttgctgc tgagcctgat tgtcgccgtt gcggcgttta acattattac
     6901 ctcgctggga ctgatggtga tggagaagca gggcgaagtg gccattcttc agactcaggg
     6961 gctgactccg cggcaaatca tgatggtctt tatggtgcaa ggggccagcg ccgggattat
     7021 cggtgcgatc ctcggagcgg cgcttggcgc actgcttgcc agccagttga ataatctgat
     7081 gccgataatc ggcgtcctgc ttgatggcgc ggcgctgccg gtcgctatcg agcctttaca
     7141 ggtcattgtt attgcgctgg tggcgatggc tatcgcgctg ctgtctacgc tttacccttc
     7201 ctggcgcgct gccgccactc aacccgctga ggctttacgt tatgaataag atcctgttgc
     7261 aatgcgacaa cctgtgcaaa cgctatcagg aaggcagtgt gcaaaccgat gtactgcaca
     7321 atgtcagttt cagcgtgggc gaaggtgaaa tgatggcgat cgtcggcagc tctggttccg
     7381 gtaaaagcac cttgctgcac ctgttaggcg ggctggatac gccaacctcc ggcgatgtga
     7441 tctttaacgg tcaaccgatg agcaaactgt cttcggcggc gaaagcagaa ctgcgcaacc
     7501 agaagctggg atttatttat cagtttcacc acctgctgcc ggattttacc gccctggaaa
     7561 acgtggctat gccgctgttg attggcaaga aaaagcccgc tgaaatcaac agccgtgcgc
     7621 tggagatgtt aaaagcggtg gggctggagc atcgtgcgaa tcaccgccca tctgaacttt
     7681 ctggcggcga acgccagcgt gtggcgattg cccgtgcgct ggtcaataac ccgcggctgg
     7741 tactggcgga tgaacctact ggtaacctcg atgcacgtaa cgccgacagc atcttccagt
     7801 tgcttgggga attgaatcgc ttgcagggca ccgccttcct ggtggttact cacgacctgc
     7861 aactggcgaa acgtatgagc cgccaactag aaatgcgtga tggacgtctg acggcggaac
     7921 tgagcctgat gggggcggag taatggcgat gcctttatcg ttattgattg gcctgcgttt
     7981 tagccgcgga cggcgacgcg gcggcatggt gtcgttgatc tccgtcattt ctaccattgg
     8041 cattgccctc ggcgtggcgg tattgatcgt cggcttaagc gcgatgaacg gctttgaacg
     8101 cgaactgaat aaccgcattc tggcggtagt accgcacggt gaaattgagg cggtagatca
     8161 gccgtggacc aactggcagg aagcactgga taacgtgcag aaagtgccgg gcattgccgc
     8221 cgctgcgccg tatatcaatt tcaccgggct ggtggaaagt ggcgcgaatc tgcgcgcaat
     8281 ccaggtcaag ggcgttaacc cgcaacagga acagcgtttg agcgcattac cctcgtttgt
     8341 ccaggacgat gcgtggagca attttaaagc aggcgaacag caaattatca tcggcaaagg
     8401 cgtggcggat gcgctgaaag tgaagcaggg ggattgggtg tcgattatga tcccaaattc
     8461 gaatcccgag cataaactga tgcagccaaa acgtgtgcgt ttgcacgttg ccggtatttt
     8521 gcagttgagt ggtcaactcg atcacagttt tgccatgatc ccgctggccg atgctcagca
     8581 atatcttgat atgggttcca gcgtgtcagg tattgccctt aaaatgacgg atgttttcaa
     8641 cgccaataag ctggtacgcg atgcgggtga agtgaccaac agctatgttt atattaaaag
     8701 ctggattggt acttacggct atatgtatcg cgatattcag atgatccgcg ccattatgta
     8761 tctggcgatg gtactggtga ttggtgtggc ctgtttcaac atcgtctcca ccttagtgat
     8821 ggcggtgaaa gacaagagtg gcgatatcgc agtattaaga acgttgggtg cgaaagatgg
     8881 tttaatccgc gccatctttg tctggtatgg attgctggca gggctgttcg gcagcctgtg
     8941 tggtgtgatt atcggcgtag ttgtttcact gcaacttacc ccgattattg agtggattga
     9001 aaagctgatc ggccatcagt tcctctccag cgatatctat tttattgact tcctgccatc
     9061 ggaattgcac tggctggacg tcttctacgt actggtcaca gcattgttgc tgagtctttt
     9121 ggcaagttgg tatccggcgc ggcgcgccag taatattgac cctgcgcgag tccttagcgg
     9181 ccagtaaagg cagtacatta aaacaaggag cggcaatgta ttacgggttt gatattggtg
     9241 gaacaaaaat tgcgctaggc gtgtttgata gcggtcggca gttgcagtgg gaaaagcggg
     9301 tgccgacacc gcgtgacagc tatgacgcat ttttagatgc agtgtgcgag ctggtagccg
     9361 aagctgacca acgttttggc tgtaaaggtt ctgtcggcat tggtattccg ggtatgccgg
     9421 aaacagaaga tggtacgctg tatgccgcca atgtccctgc tgccagcggt aaaccgctgc
     9481 gtgccgacct gagcgcacgt cttgatcgcg atgtacgcct tgataacgat gccaactgtt
     9541 ttgccctttc tgaagcatgg gatgatgaat ttactcaata tccactggtg atggggttga
     9601 ttctcggcac cggcgttggc ggcgggctga ttttcaacgg taaaccaatt accggtaaaa
     9661 gctatattac cggcgagttt ggccatatgc gtctgccggt tgatgcgtta accatgatgg
     9721 ggctggattt cccgttacgc cgctgcggct gtggtcagca tggctgcatt gaaaattatc
     9781 tgtctggtcg cggttttgcg tggctgtatc aacactatta tcatcaaccg ttgcaggctc
     9841 ctgagatcat tgcgctttat gatcaaggcg atgagcaggc aagggcgcac gttgagcgtt
     9901 atctggattt attagcggtt tgtctgggaa atatcctgac cattgttgac cctgacctgg
     9961 tcgtcattgg tggtggctta tcgaatttcc cggcaatcac aacgcaactg gcggaaaggt
    10021 tgcctcgtca tctcttacct gtagctcgtg ttccgcgcat tgaacgcgcg cgccacggtg
    10081 atgcgggagg aatgcgtggt gcggccttcc tacatctaac cgattaatca acagaggttg
    10141 ctatgctgtc gcgtcggggt catcggttaa gtcgttttcg taaaaataaa cgccgcctgc
    10201 gcgagcgttt gcgtcagcgt atttttttca gagataaagt ggtgccggaa gcaatggaaa
    10261 aaccaagagt actcgtactg acaggggcag ggatttctgc ggaatcaggt attcgtacct
    10321 ttcgcgccgc agatggcctg tgggaagaac atcgggttga agatgtggca acgccggaag
    10381 gttttgatcg cgatcctgaa ctggtgcaaa cgttttataa cgcccgtcgt cgacagttgc
    10441 agcagccaga aattcagcct aacgctgcac atcttgcgct ggcgaaactg caagatgctc
    10501 tcggcgatcg ctttttgctg gtgacgcaaa atatcgataa cctgcatgaa cgcgcaggca
    10561 ataccaatgt gattcatatg catggggaat tgctgaaagt acgttgttcg caaagtggcc
    10621 aggtcctcga ctggaccgga gacgttacgc cagaagataa atgccattgt tgccagttcc
    10681 cggcaccctt gcgcccgcac gtagtgtggt ttggcgaaat gccactcggc atggatgaaa
    10741 tttatatggc gttgtcgatg gccgatattt tcattgccat tggtacttcg gggcatgttt
    10801 atccggcggc tgggtttgtt cacgaagcga aactgcatgg cgcgcacacc gtggaactga
    10861 atcttgaacc gagtcaggtt ggtaatgaat ttgccgagaa atattacggt ccggcaagcc
    10921 aggtggtgcc agagtttgtt gaaaagttgc tgaagggatt ataaagcggg aaaaattgcc
    10981 tgatgcgcta cgcttgtcag gcctgtgaag gggcgcaatt aattgaattt gcgggttttg
    11041 tgggccggat aaggcgttcg cgccgcatcc ggcataatac acgcgaactt tactgcctct
    11101 tttaccgttt actcgtccat agtaacgcgg caataaacgt acagacacca atcagccctg
    11161 cccagagtgg cgatattctg gtaatcgcaa agctgttatc gatatacaaa cataaagcct
    11221 gataacccat attggcaaca gccatcgcca ctgcaaattt taacgcataa taaaggcgcg
    11281 ttgtgtagtt aaaaaatgca gcaaagacga tgaatgcaac tgcccaggca aaaaggccta
    11341 atgtcgtggt actgccgggt ggatcatttt catgctgttg tccgggatgc ggataatatg
    11401 tcagtggtgg cgtcggttgt tttaatatct gtgcttgtaa aacagccaat gcattattga
    11461 taccctgata ccagaggtcc gcatgtaaat aagccagcgt gtgattttct accagaaggc
    11521 cgagatgttt atcgtccaga ccgctttcaa cctggccgtt ggttatcata attaaccgtg
    11581 gctcgcgggt agtaaagagt aacagaacac ttccttttac attactcatt gaagatgccg
    11641 catgcgccag catcgctttg ccattttgcc tgagactggc ttcattatta atgtcgggta
    11701 aaaccacaat aaacggtgca actccggttt gttggcgaaa aagctcggct ttatgcgcga
    11761 tctcttcagc atctggtaca gtaccggtaa catccgtaaa aagtgtcttc attattggaa
    11821 ttaagggctt actggttgcc gtcagaggca ggagtatcag taaactgagc aatattatga
    11881 tttttttcat gggcgggact tccgtttcct gcgtgtcttt ttagaggagg gcatactgat
    11941 taataaaccg acggagataa aaataacgcc cataatgccg ataaaccccc atttaaaaaa
    12001 attaacccac gggttattta tctcagcctt ttcaggatgt cctggagaat aataaacatt
    12061 aactttatcg ccttcgctgc cttcataaaa atcgctgcca atactcgaat tgaaaattag
    12121 cgtgtaatct ggcgtcggac gaaaggcaac gacgggatac cacgaaccat ttttacccgt
    12181 gcgatgagaa tgactggagt gccagacggt atcaacaatg acgccttcag tatgaatcgc
    12241 gtctttgcta taggtgaact ggttataaaa gataacaaca gaaatgagga tcagtaccag
    12301 accgataccg aagaatatac gaaagaaaac tctactgtcc tggctcatta gccacatcct
    12361 tgctaaataa aaaacgggcg gtaatatcac cgcccgcttg ctgaattaac gtcctgcttt
    12421 cagcttctga taatactctt cataaatgct gctggctgcg ccaacgtcat tctgccattc
    12481 accattttta atggtttcag catccgggta gagcgtttta tcgttcgcca cttccggact
    12541 taacagctta cgcgccgcaa ggtttggcgt tggataaccg atagtttcag caacctgttt
    12601 tgccacatcc gggcgcagca ggaagttgat caatttcagc gcgccttctt tgtttttggc
    12661 atttgccggg atcgccaggc tgtccatcca gaaaataccg ccttctttcg gccacaccac
    12721 atcaattggc gtacccgcct ggcgtgcaac gaaagcagaa ccgttccaga tcatgccgag
    12781 gttaacttcg ccttccatat acgggttcgc cgggttatcg gagttaaacg ccgcgacgtt
    12841 tggcatcagc tttttcagct cgttgtatgc agcttcaatc tctttcggat cggtggtgtt
    12901 accggagtag cccagcttac gcagcgccat ctggaacact tcacgggcat cgtcggtcag
    12961 cagcaggctg cctttgtact caggcttcca cagatcggcc cagctggtga cagatttcgg
    13021 atccaccgca tcaccgttaa cgccaatcgc cgtcgcaccc cagatatacg gaatggaata
    13081 gtcgttattc gggtcaaacg gcttgttgag catgtctgga tcgaggttgc tgaaattgct
    13141 taacttcgac ttgtcgatct tctggatcat cccttcttta cgcattttat cgacgtaata
    13201 ggttgaagga accaccaggt cataggcacc gtctttgtac gttttcagct tcgcgtacat
    13261 ggtttcgttc gactcgtaag tcgaatagat aaccttaata ccggtttctt tggtgaactg
    13321 ttcaagcagt cctggcggca cgtactcggt ccagttgtag aaatacagcg tgttgttgtc
    13381 atcggcgtga gcggcgctca tgcccagtgc cagagcaccc gccgcgagca ggtggcgtga
    13441 ccattttttc atttaacgtc ccctgtgtta cctttcgttt tatcacgagc aataagctgg
    13501 ctggcaatta ccatcaccag cgacagcacc agtaatatgg ttgccagcgc gttaacttcc
    13561 ggcgatacgc cgactttgac catcgaataa atttttaacg gcagaatttc ataactcggc
    13621 ccggtgacaa acgaagaaac caccacgtcg tccatcgaca gggtaaagct taacacccag
    13681 cccgccgcta ccgctggcat tgccagtggc agaatgattt ttcgcagaat ggtaaattcg
    13741 ctggcaccga gatctttcgc cgcttccagc attcgcacgt caaaaccttt caggcgcgaa
    13801 tacactgtca ctaccacaaa tggcaggcag aaggtgatat gcgagaacag cagcgaccag
    13861 aagccaagct gaatacccag cagcataaac agcaccagca gcgaaatcgc cattacgata
    13921 tctggtgaca tcatcaccac aaacagcatt ccgctaacga acggcttacc acgaaagcga
    13981 taacggtaca gcgcaactgc cgtcagtgaa ccgataagcg tagcaaacgt cgccgaaaac
    14041 accgccattg taagtgaatg ctgcgctgcc tgtaacaggc tgtcgttgtt catcagcagg
    14101 ctataccatt tggtggtaaa accctgccag ttgatgccaa agcgcgagct gttaaaggag
    14161 ttcacaatca aaataatgat tgggatatac aggtacgcgt agatagcggt cataaaaccg
    14221 ccgcgaagca gtcgaccgat cattcgagtt ccaccttctt attcagcaaa cgagaagcgc
    14281 gccagtaaac cagcaacatc aggcccatta cgatagtcag cgtaatgctg gtggctgcac
    14341 caaacggcca gtcacgaata ttgaggaact gtaccttgat gacgttaccg atcagcaggt
    14401 ttttcgcacc gcccatcagg tcggacacat agaacaggcc catcgctggc agcatcacca
    14461 gcagacatcc ggcaataatt cccggcatcg tcagtgggat aatgatgcga ataaaggtct
    14521 gcaacttgct ggcaccgaga tcgcgcgccg cctcaagcaa cggcttatcc agtttttcga
    14581 tactggagta cagcggcatc accataaacg gcagcagaat gtaaaccaga ccgataatca
    14641 ccgcactagg cgtgaacatg atgcgaattg gtgtatcgat aacgcccagc cagagcaaaa
    14701 actcgttgag atagcctttg gtgctgagga aaattttcag cccgtaaata cgaatcaatg
    14761 agttggtcca gaacggaaca atcagcagaa acagcagcag cggacgcacc ttgtgtggca
    14821 gcttcgccag aaaccaggca aaaggatagc ccagcaccag gcaggcgagg gtggcgatca
    14881 gcgccatatt cagcgagtgc aatagcactt caaaatagag cggatcgagc agacgcgtgt
    14941 agttatccag cgtaaagacc attttgacga aactggcgtc gtcgcgggtc aaaaagctgg
    15001 tgccaatgat catcaggttg ggcagaaaga taaacaacac aagccaaccg acaatagtga
    15061 caatcactac attctggaac ttacttgtgt tcttcatcag ccagtacgac ctcccagctt
    15121 tctacccaat taatggccat tttttggtcg agagagtggt caaagtcagg atcgtcttca
    15181 ttgaagaatt cgctgaccat caccatcttg ccattttcca gctcaacaac cgactccagc
    15241 gtcatgcctt tgtagttacg ctcgcgaacg taaccaatca gcccttcggc gtggttgtcg
    15301 tcgttaatct cttcaacacg taagtcttcc gggcgcagca gaacatgcag tttttgcccc
    15361 ggttcaacgg cgaagttaac gtagatatta cattcgcggc cttcaacgtt ggcgcgtacg
    15421 cgctgctcgt cgagacgttc gatgacagtg gcgttaaaca tattgatttc gccaataaac
    15481 ccggcaacaa acaggttttt cggctcttcg tagatttcac gcggcgtgcc gtcttgctca
    15541 atgcgaccat cgcgcatcac cacgatcctg tccgacatgg taagtgcttc ttcctggtcg
    15601 tgagttacga acacgaatgt aatgccaagc ttacgctgta acgctttcag ctcgttctgc
    15661 atttgcttac gcagtttgta atccagcgct gagagcgact catccagcaa caacagacga
    15721 ggcttgttaa ccaccgcgcg agcaatggcg acgcgttgct gttgaccacc agagagctga
    15781 tgcggtttgc gttgagcgaa ggtttccagc tgcaccatcc gcagggcttc catcacgcgg
    15841 ggcgtaattt cagctgcggg ggttttttgc atgcgcaacc caaaggccac attttcgaac
    15901 acggtcatgt gggggaaaag tgcgtagctt tggaaaacag tgttcacata gcggttttcc
    15961 gccggaacgt gggtgatgtc ctcgttatcc agcatgatgc gtccggaatc aacagtttcc
    16021 agacctgcaa tcaggcgaag aacggttgtt ttaccgcagc cagaagggcc aagcagcgtg
    16081 aggaactcgc cattgttgat agtcagatcc agctggggaa tgacctcttt accatcaaag
    16141 catttgcgaa ttcccgccaa ttgcaccagc ggtgaaagcg aactcggttg tttattcaat
    16201 tttttactct gtcccatgta aacgcaacgg atggcttacc gatgcggggt ttgtggttaa
    16261 ccaccttggt gactcttaat gagggcggta attctacggc aaaccgcttg aatcgccaat
    16321 ctttgttgtg aattactggc ttagctttat attcattaag gtaatgctga taaatattcc
    16381 cgcttgcagg gctaaaagtg acctgacgca atatttgtct tttcttgctt cttaataatg
    16441 ttgtcacaaa aagtgagggt gactacatgg ataaactact tgagcgattt ttgaactacg
    16501 tgtctcttga tacccaatca aaggcagggg tgagacaggt tcccagcacg gaaggccaat
    16561 ggaagttatt gcatctgctg aaagagcagc tcgaagagat ggggcttatc aatgtgacct
    16621 taagtgagaa gggcactttg atggcgacgt taccggctaa cgtccctggc gatatcccgg
    16681 cgattggctt tatttctcat gtggatacct caccggattg cagcggcaaa aatgtgaatc
    16741 cgcaaattgt tgaaaactat cgcggtggcg atattgcgct gggtatcggc gatgaagttt
    16801 tatcaccggt tatgttcccg gtgctgcatc agctactggg tcagacgctg attaccaccg
    16861 atggtaaaac cttgttaggt gccgatgaca aagcaggtat tgcagaaatc atgaccgcgc
    16921 tggcggtatt gcaacaaaaa aacattccgc atggtgatat tcgcgtcgcc tttaccccgg
    16981 atgaagaagt gggcaaaggg gcgaaacatt ttgatgttga cgccttcgat gcccgctggg
    17041 cttacaccgt tgatggtggt ggcgtaggtg aactggagtt tgaaaacttc aacgccgcgt
    17101 cggtcaatat caaaattgtc ggtaacaatg ttcacccggg cacggcgaaa ggagtgatgg
    17161 taaatgcgct gtcgctggcg gcacgtattc atgcggaagt tccggcggat gaaagcccgg
    17221 aaatgacaga aggctatgaa ggtttctatc acctggcgag catgaaaggc accgttgaac
    17281 gggccgatat gcactacatc atccgtgatt tcgaccgtaa acagtttgaa gcgcgtaaac
    17341 gtaaaatgat ggagatcgcc aaaaaagtgg gcaaagggtt acatcctgat tgctacattg
    17401 aactggtgat tgaagacagt tactacaata tgcgcgagaa agtggttgag catccgcata
    17461 ttctcgatat cgcccagcag gcgatgcgtg actgcgatat tgaaccggaa ctgaaaccga
    17521 tccgcggcgg taccgacggc gcgcagttgt cgtttatggg attaccgtgc ccgaacctgt
    17581 tcactggcgg ttacaactat catggtaagc atgagtttgt gactctggaa ggtatggaaa
    17641 aagcggtgca ggtgatcgtc cgtattgccg agttgacggc gcaacggaag taagcgaaaa
    17701 gggatgcggc atgtgatgcc gcatccggct taaatccaaa cttacccttc gaagaaccag
    17761 tatccgctat tgaccagcgc cgcgagcatc gcgaggaatg acggatcttc cagcgcatcg
    17821 ccaaaattct ccgcggtcag cgcaatgttg ctggcgagcg catccagtgc cggacggtgc
    17881 ggggaatcga tcttctcacc attggcatac acgtcgtcgc caatgcgcaa tacgcgcaga
    17941 ccccccaggc gcaccagcac atcaccttgt ttcagcgcat cgtagatttc atccggctga
    18001 taaggcggct ctggcggcgc gatatccagt tcatgacgtg actgggatat aaactcgcca
    18061 aaccattgct taaaatgttc tggctggttg atcaattcga gcatcatctc acgcagttta
    18121 tccatctctt gcggcagaac gtccgcagga tgagcgcgag gtggaacatc aggatcgctg
    18181 tagtagttgc cgcccagttc acgttgcagc acataatcgg caaatccgct aatcagttcc
    18241 cgcgtatttg gcgcgcgaaa acccacggaa tagttcatcg cattttccag cgcgtagcct
    18301 tcatgcggga atcctggcgg aatataaaga atatcgccag gctccagctc ttcatcgatg
    18361 atggcttcga acggatcgac ctgtaacaga tccgggtgag ggcagtgctg tttcatttgc
    18421 agcttttcgc ccactcgcca gcgacgacga ccggtaccct gaatgataaa cacgtcgtac
    18481 tgatcgagat gcgggccgac gccgccgccc ggtacagaaa aagagatcat cagatcatca
    18541 atacgccagt ccggtagttc acggaacggt cgcattagcg cggcggtcgg ctcatgccag
    18601 tggttcactg cttgcactaa caatgaccag ttagtttcac cgagatgatc gtagctttcg
    18661 aatggaccgt ggctgacctg ccatttgcca tcctgatgac tgaccagtcg actgtcgact
    18721 tcgctttcca tcgccaaacc cgccaactcg tctggagaga tcgggtcaat aaaattatta
    18781 aagccgcgtt ttaacaccac cggacgtttc tgccagtgac gttcaagaaa atcgggccag
    18841 ttaagagtga gttggtattc catgttaagc atccacaggc tggtatctgc aaccgattat
    18901 aacggatgct taacgtaatg cgtgaagtat ggacatattt attcatcttt cggtgcagaa
    18961 tgctggcgac caaaaatcac ctccatccgc gcaccgccca gcatgctctc tccggcgacg
    19021 attttaccct catattgctc ggtgatttcg cgggctaccg ccagccctac accttgccca
    19081 gggcgtaaag tatcaacccg ttgaccacgg tcgaaaatga cctctcgctt gcttaatgga
    19141 ataccggggc catcatcctc gaccacaata tagagatgct cgtctgtttg ccttgcagat
    19201 atttcgacaa actcgaggca atatttacag gcattatcca gcacgttgcc catcacctcg
    19261 acaaaatcgt tctgctcacc gacaaagctg atctctggcg aaatatcgag agagatattg
    19321 acccctttgc gttgatacac tttgttcagc gccgaggtga gattgtccag cagtggggcg
    19381 accggatgca gctcgcggct aagcaatgtc ccgccgcgca tactggcacg atgcaggtag
    19441 tagccaattt gctgtgaaat gcggctgatt tgctccagca ttaccggctc agcatcactg
    19501 acgctcatct tctcactacg cagagaacgc agcgtacttt gcagcaccgc cagtggcgtt
    19561 ttcagactat gggtcaggtc ggtgagcgtc gtacgatatt tgtcgtaacg ttcgcgttca
    19621 ctttttaaca gtcggttcag gtttcgtacc agactagtca gttcccgcgt tgtggctgga
    19681 ttgagcaatt cgcggttatg ttcttccagt tcgcggactt cttttgccag ggcttcgatg
    19741 gggcgtaaac tccaccaggc ggcgacccac agcagcggga tcactaacag cagattggct
    19801 gagagcacat agataaacca gctccagacc atataggaac tttttagctc caccggaatg
    19861 gtatccacca ccacaatggt taactttggc atccgcgatg ttgccgggta gacgtttacc
    19921 gccaccgagt gggtcatctc cgcgtcgtca tcatcttccc gcacttcctg caactgttgc
    19981 tgtatcgaat gatctccact cagcaagagg ctggtatcgt taacatccgc ttcaatttca
    20041 tgaaaaccgt tcgattttag ccagtcgggc tggatcatct tcatcagcca gggcacgtca
    20101 cgttgcgccc ataaaagctg cccgttctca tcataaatta gtgtcatcgt ggggctttgc
    20161 ttgtcgatat tttcgggtaa ctcgacatgc aacttattgt tttcccactt cgcaagggta
    20221 tagaacagat tgctctcgcc acgtaacagc cgaaacgtgg ttttatcgaa actgacgcta
    20281 taaccgatca gcgcaaccat tccgtaggca agcgaaagca ccaatactac tgctgccgtt
    20341 gctaacagaa aacgtacccg cagcgagagc gggaaaaaaa gacgcagtaa ttttttcatc
    20401 agcgcaattc gaacagatag ccctggccgc gaacggtggt aatcacttct tgcggatatt
    20461 gtgcctgaat ttttttgcgc agacgtccca tcagtacatc aatggtatgg ctttcccgca
    20521 gctccgcatc cgggtagagt tgtagcatta acgaatcttt gctgaccact ttgccattat
    20581 tgcgtatcaa cgtttccata atggtgtatt cgaacgcggt cagtttgatc acttcgtcat
    20641 taatagataa ttcacggcgg gagagatcaa cctgaaacgg tggtagcgaa atgacctgtg
    20701 aagccagacc gctattacgc cgcattaatg cctgcattcg cgccatcacc tcttcaatat
    20761 gaaacggttt agtcacgtaa tcatcagcac cggcacttaa tacttcgact ttgtcctgcc
    20821 agctctcacg ggcggttaat accagaatcg gcagtgaaac atcattgcta cgccagcggc
    20881 gaatcagtga cagaccatcc tcgtcaggca atccgagatc gacaatcgca atatccggta
    20941 aatgttcatt gagataataa tcggcttctt tggcatcttc tgcgtcatcg acctgatgac
    21001 cagcatcctg tatctgaact ttaagatggt gacgtaacaa cgcattgtct tcaacaacca
    21061 gtacgcgcat ttttatttct ccctgtctta attattaaaa tagtgtaacg cgattatgtg
    21121 gttatggggg taaacattaa ataaaccagc gggaagggga ggtaaagtga aaaaataaaa
    21181 agcggataat cttaataagc aggccggaca gcatcgccat ccggcactga tttgaggctt
    21241 atttcagctc atcaaccatg gtgatggcgc gaccaatata gttagccggc gtcatcgctt
    21301 tcaggcgggc tttctcttct tctggcagcg cgagaccgtc gataaattgc ttcatgcctt
    21361 cggcgtcaac gcgcttaccg cgagtcagct ctttcagctt ctcgtacggt ttttcgatgc
    21421 catagcgacg cataactgtc tggattggct cagccagcac ttcccagttg tgatccagct
    21481 cctccagcag atggtcatgg ttcacttcca gtttgctcac gcctttcagg gtggattgat
    21541 acgcaatcag cgcataaccg atacccacgc cgaggttacg cagcacggtg gagtcggtca
    21601 ggtcacgctg ccagcgggaa actggcagtt tgcttgccag gtgctgcaat accgcgttgg
    21661 aaaggcccag gttcccttcg gagttttcga agtcgatcgg gttaacttta tgcggcatgg
    21721 tggaagaacc aatctcacca gcaatggttt tctgtttgaa gtggttaagg gcgatataac
    21781 cccagacgtc acggtcaaag tcgatcagaa tggtgttgaa gcgcgcaacg caatcaaaca
    21841 gttcggcaat gtagtcgtgc ggttcgatct gggtggtgta cgggttccac tgaataccca
    21901 gcgaggtgac gaactcttcg ctgaactgat gccagtcaac ttccgggtaa gcggcgatgt
    21961 gggcgttgta gttaccgaca gcgccgttga ttttgccgag gatctccacc tggttaagct
    22021 ggcggtactg gcgctccata cggtaggcga cgttagccat ctctttacca atggttgacg
    22081 gcgtggctgg ctgaccgtgg gtacgggaca gcagcgggat atcgcgatac tgaacggcga
    22141 gatctttaat gccatcaatc agttgacgcc agtacggcag gatcacttca tcacgcgcgg
    22201 ttttcagcat taatgcgtgg gagaggttat tgatatcttc cgaagtacag gcaaaatgga
    22261 tgaattcaga aaccgcgtgc agttccggga tatccgccac tttttctttc aggaaatact
    22321 caaccgcttt aacgtcgtgg ttagtggtac gctcgatggt tttgatgcgt gctgcatctt
    22381 cttcgctgaa actggcgaca attgcatcaa ggtaaccgat tgcgtcggca gcaaaagcag
    22441 gaacttcctt gatcgctgcg tgcgcggcca gtttttgcag ccaacgtact tcaacttgta
    22501 cacggaattt cagcaaaccg tattcgctga aaatcccgcg cagcgcgctg actttatcgc
    22561 cgtagcgtcc atcgacaggg gaaacggcgg tcagtgagga taattccata gatcacaact
    22621 ccggggttaa atgagcaaga atttgttttg cctgagtggt caggcgatta cgagaaaaca
    22681 tcagttgcag acgaccgccg ccgacctggt gccagagcac ggcggcgcga atgcctgcca
    22741 gcagggttgc gcgaactttc gcctgcacct gtgggctttg cagtacagca ggggaacccg
    22801 tgacctgaat gcgcgggcca agcgggctga taacatcaac atagatggca gccatcgcgc
    22861 tcatcagcgt ttcggactgt aaatcgaagt gttcgagctg gcgttgcagg ccgttgatcc
    22921 ggttgcccag agtgtcgagc gcacctttcg ctgaggagag tttgcgctca agcaccatca
    22981 gactgagtgt gtagcgggtt aattcggcgt ttaagccttg gcgactgctg gcattgagta
    23041 cgccgagcaa ggtttccagc ccgacgcgca ggttggcttc gctgccgcca aaaaccgcca
    23101 gcgttgagct ggggttcata tcgataatac tgttgagtga gacgtgtagc gcatcgccat
    23161 cacaatgccc ctggtgagcg agttgttgca ccaggcgtgc cgactgacaa ataccggcca
    23221 gggcgagggt gatgtcatag taattctttg ccacgttcac tgcttccttg ttttaagtaa
    23281 agataataat cagaccggca gcggcagacg ctgctcaata ataccgccac cgaggcacac
    23341 ttcaccgtta tagaaaacgg cagactggcc cggcgtcacg gcggcaaccg gctcatcgaa
    23401 aatcacttca atgcgatcat cgtccagcgc cttgacggtg cacgggatgt cggtctggcg
    23461 atagcgggtt tttactgtgc aacgcatagt accggtgaac ggttcgcgat cgacccagtg
    23521 cagctgctgg gcaatcagac cgacagacat cagacgcggg tgttcatggc cttgcgcaac
    23581 aatcagaatg ttgttttcga cgtctttatc gaccacatac cacggttctt cggtgccgtc
    23641 tttggtgcca ccgataccca gacctttacg ctgaccgagg gtgtgataca tcagcccctg
    23701 gtgctcacca atttcatcgc catcgacggt aatgattttg cccggttgcg ccgggagata
    23761 acggcccagg aactcgcgga atttacgttc gccaatgaag caaatgccgg tagagtcttt
    23821 tttcttcgcg gtgaccagac caagatcttc agcaatctta cgcacctgcg gtttttccag
    23881 ttcgccgacc gggaacaggc tttgcgcaat ctgctcatgg ctgagcgtat aaaggaagta
    23941 gctctggtct ttattgctgt ccagaccacg cagcaggcgg ctcttgccat cgacatctgc
    24001 ccgacgtacg taatgaccgg tagcgatata atcggcacct aaatcttcgg cggcaaattc
    24061 gaggaaagct ttaaatttga tctctttgtt gcacagaata tccggattcg gcgtgcgacc
    24121 ggctttatat tcggcaagga acagttcgaa gacgttgtcc cagtactcgg cagcaaagtt
    24181 aacggtgtgc agttcaatgc cgagcttgtc gcagacagcc tgggcatcag ccagatccgc
    24241 tgccgctgtg caatattcct caccgtcgtc ttcttcccag ttcttcataa acaggccttc
    24301 gacctgatat ccctgttgtt gtaacagcca ggcagaaacg gaggaatcga caccgccgga
    24361 catgccgacg attacttttt ttgcggtttc agacattgga tcactcacga cattgaactt
    24421 caaggcggcg tattctatca cgcagccttt tagatgacac cctttgtaaa aggccagtta
    24481 aaatcgccaa tcatctccag cggatagcgt tgcccgcttt gataacaacg aatactttcc
    24541 gccaccagcg gcgaacgaag atttgacgcc tgtaaaatt
//
LOCUS       DAOFSV010000058        24126 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.058, whole genome shotgun sequence.
ACCESSION   DAOFSV010000058 DAOFSV010000000
VERSION     DAOFSV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.058"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..764)
                     /gene="vgrG"
                     /locus_tag="QBH27_002467"
     CDS             complement(<1..764)
                     /gene="vgrG"
                     /locus_tag="QBH27_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ7014770.1"
                     /translation="MNVQFFDHAHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIEFT
                     STDKAIEPESVLMQDGAFSLSAPPVQGMPVQVPLRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMT"
     gene            complement(776..3412)
                     /gene="tssH"
                     /locus_tag="QBH27_002468"
     CDS             complement(776..3412)
                     /gene="tssH"
                     /locus_tag="QBH27_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDQ7014771.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSAQPGAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGRVIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLGWDEEDGIVLEFDEK"
     gene            complement(3603..4094)
                     /locus_tag="QBH27_002469"
     CDS             complement(3603..4094)
                     /locus_tag="QBH27_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDQ7014772.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            complement(4152..5804)
                     /locus_tag="QBH27_002470"
     CDS             complement(4152..5804)
                     /locus_tag="QBH27_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDQ7014773.1"
                     /translation="MRNALKQTVLLWTLLLLLTTWLGFFAVSAVVKYAVVVILVAVTA
                     AVMAYWHKKKGQVVADDVWPSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     PVSDEEQLVAQVERLLTLRPAWASQFAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLCYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMDVTASLPVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            complement(5817..6482)
                     /gene="tssL"
                     /locus_tag="QBH27_002471"
     CDS             complement(5817..6482)
                     /gene="tssL"
                     /locus_tag="QBH27_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HDQ7014774.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLVLAGVWWGLHYWLNVLVDELLPQ
                     SLR"
     gene            complement(6479..7816)
                     /gene="tssK"
                     /locus_tag="QBH27_002472"
     CDS             complement(6479..7816)
                     /gene="tssK"
                     /locus_tag="QBH27_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDQ7014775.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            complement(7834..9372)
                     /gene="tssC"
                     /locus_tag="QBH27_002473"
     CDS             complement(7834..9372)
                     /gene="tssC"
                     /locus_tag="QBH27_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDQ7014776.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDS
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDVSKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            complement(9431..9931)
                     /gene="tssB"
                     /locus_tag="QBH27_002474"
     CDS             complement(9431..9931)
                     /gene="tssB"
                     /locus_tag="QBH27_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDQ7014777.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            complement(10567..10643)
                     /locus_tag="QBH27_002475"
     tRNA            complement(10567..10643)
                     /locus_tag="QBH27_002475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10607..10609),aa:Met,seq:cat)
     gene            complement(10677..10753)
                     /locus_tag="QBH27_002476"
     tRNA            complement(10677..10753)
                     /locus_tag="QBH27_002476"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10717..10719),aa:Met,seq:cat)
     gene            complement(10787..10863)
                     /locus_tag="QBH27_002477"
     tRNA            complement(10787..10863)
                     /locus_tag="QBH27_002477"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10827..10829),aa:Met,seq:cat)
     gene            11072..12169
                     /gene="mltA"
                     /locus_tag="QBH27_002478"
     CDS             11072..12169
                     /gene="mltA"
                     /locus_tag="QBH27_002478"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDQ7014778.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            12246..13052
                     /gene="tcdA"
                     /locus_tag="QBH27_002479"
     CDS             12246..13052
                     /gene="tcdA"
                     /locus_tag="QBH27_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDQ7014779.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(13103..13546)
                     /gene="csdE"
                     /locus_tag="QBH27_002480"
     CDS             complement(13103..13546)
                     /gene="csdE"
                     /locus_tag="QBH27_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDQ7014780.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(13546..14751)
                     /gene="csdA"
                     /locus_tag="QBH27_002481"
     CDS             complement(13546..14751)
                     /gene="csdA"
                     /locus_tag="QBH27_002481"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDQ7014781.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEASRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            14943..15170
                     /locus_tag="QBH27_002482"
     CDS             14943..15170
                     /locus_tag="QBH27_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDQ7014782.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15521..16438
                     /gene="gcvA"
                     /locus_tag="QBH27_002483"
     CDS             15521..16438
                     /gene="gcvA"
                     /locus_tag="QBH27_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDQ7014783.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGENPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            16457..16852
                     /locus_tag="QBH27_002484"
     CDS             16457..16852
                     /locus_tag="QBH27_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDQ7014784.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            16845..17945
                     /gene="rlmM"
                     /locus_tag="QBH27_002485"
     CDS             16845..17945
                     /gene="rlmM"
                     /locus_tag="QBH27_002485"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDQ7014785.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(17989..18720)
                     /gene="fucR"
                     /locus_tag="QBH27_002486"
     CDS             complement(17989..18720)
                     /gene="fucR"
                     /locus_tag="QBH27_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDQ7014786.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(18778..19200)
                     /gene="fucU"
                     /locus_tag="QBH27_002487"
     CDS             complement(18778..19200)
                     /gene="fucU"
                     /locus_tag="QBH27_002487"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDQ7014787.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(19202..20620)
                     /gene="fucK"
                     /locus_tag="QBH27_002488"
     CDS             complement(19202..20620)
                     /gene="fucK"
                     /locus_tag="QBH27_002488"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDQ7014788.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAVVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATSIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAELYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(20729..22504)
                     /gene="fucI"
                     /locus_tag="QBH27_002489"
     CDS             complement(20729..22504)
                     /gene="fucI"
                     /locus_tag="QBH27_002489"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDQ7014789.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(22537..23853)
                     /gene="fucP"
                     /locus_tag="QBH27_002490"
     CDS             complement(22537..23853)
                     /gene="fucP"
                     /locus_tag="QBH27_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDQ7014790.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         5517 a         6334 c         6485 g         5790 t
ORIGIN      
        1 tctgtcatca tctctgctgt ggcggtgcgg tagttgtagt cccgagtggt gacgcttttt
       61 tccaccacgc tgtacgcggt attcagcccc cataccgctt ccgtttcacc atcaaacagc
      121 cctgaaggat ggcgaagcgg cagtgtcagc ccgcgctcgt agccgctctg gtcgtcataa
      181 aactcgacaa cttcgatttt cagccgggcg tcggtggcaa agcggaacca gatacccact
      241 tccgacagca ggcgtgagac aaacgtcagg tcgtcctcac cgtactgcat cacctgttcg
      301 cgggacgggt attcgctttt gaggttaaac acgaagtcct gaccgcgcat ctggtgccgc
      361 tcgcgcagga ttttttcgac gatttgcggc accgtctggt tctggtagat ggcgttctgg
      421 cggctgcggg taagcagcgc catacggggt tcaaggcgca cttcgtaacg ggcttcgtcc
      481 tgtgacgagg agaggtgttt aaagccggta atgacgccgt gcagggtgcg cagggggacc
      541 tgcaccggca tcccctgtac cggcggggcg ctcaggctga atgcgccgtc ctgcatcagt
      601 accgactccg gctcgatggc tttatcggta ctggtaaact caatgtcgta gcggaagggt
      661 gagctgagct gttcgtggcc tgtaaaagtc agcacgtcca ccgggctttt caggccgcga
      721 attttgagct tgtggtgtgc gtggtcgaag aactgtacgt tcataagagc ctccattatt
      781 tctcatcaaa ttccagtaca atcccgtcct cctcatccca gccaagccgc agtcgggcag
      841 gcttatgatg cacggcctgc tgtgccagca actgctggct taagaccggc aggatttgct
      901 ggttcaggag gctgtcgata ttgcgcgccc cggtatccgg cagcaggcag gcggcggtaa
      961 gctggtcata caggctgtcg ttgatttccg tttccagtcc gtagtgctgg tgaagacggc
     1021 ggaccacctg cgccagtttc atttccacaa tggtgcgcat cgcctcctgt cccagcgggc
     1081 ggtaaatcac cgtctgaaaa cgcgccatca gtgcaggctg aaaatggtct cgcagaaggg
     1141 gatacagcag ttcatgcagg tctgcgtccg tggcgtccgg tttttcctcc agcagttgca
     1201 tgatgtggtc gctgcccaga ttggcggtca tcagaatgac ggtgttgcgg aagtcgatta
     1261 cccgcccttc gccgtcgcgc ataaagcccc ggtcgaacac ctgatagaac aggttcatca
     1321 cgtcgcgatg cgccttttcc acttcgtcga gcagcaccac gctgtaaggg cgtttgcgaa
     1381 cggcttcggt aagtacgcca ccctgaccgt aaccaacgta gcctggaggc gagcctttca
     1441 actgggaaac ggtatgcggt tcctggtatt ccgaaagatt aatggtgata agggattttt
     1501 caccaccaaa cagggtgtcg gccagtgtga gcgcagtttc ggttttgccg gtgccgctgg
     1561 ggccggtcag caggaatacc ccctgtgggc cgttctccgg cgtgaggccg gttttagccg
     1621 cccgcagccg ctgtgcgatg gcgcagagcg ccgggctttg cccgacaacg cggttttcaa
     1681 gactctgttc catactgagc aggtcgctct gctcgtccct gagcagggaa gagagcggga
     1741 tgccggtcca gtccgccacc accgtggcga cggcgcgggc gtccacatcc agcgccagca
     1801 gcggtgcaaa gccctgatgc gtccgcagtt gctgttgcag ttcagagctg tctgttcctt
     1861 cctgacgcaa cgtcataatc tgctgcgcca gtgccttttc ctgctgatac tgcgcttcca
     1921 gatgcccggc ttctgcctgt agctcatcac gcaaatcggt cagttccgcc agccgctccg
     1981 ggctggtttc gggataaaac acgctgtcct gctcaatcgc ctcccgctcg atatccagcg
     2041 ccgccagtcg cgcatgaagc tgcgtcaacg gctccggcag agtgtcaagg ctcatgcgga
     2101 ctcttgcccc ggcggtatcc agcaaatcaa ccgctttgtc cggcaactgg cgtccggtca
     2161 gatagcggcg cgacaggcgc acggcggtct gaatggcgct gtccagcata tgtacgccgt
     2221 ggtgctgtgc atagcgggcc ttgagtcccc gcagcatcag gcaggcggtg tcatcatccg
     2281 gctcgtcgac tttcaccatc tggaagcggc gttccagcgc ggcgtcgcgc tcaaaatact
     2341 gtttgtattc gctccacgtg gtcgccgcga tggtgcgcag ttcgcccctt gccagcgccg
     2401 gtttcagcag gttcgccgca tccgcgccgc ctgcctgatt acccgcaccg ataatggtat
     2461 gggcttcatc aataaacagc agtaccggct ccggtgattt ctgcactgca tcgatgacat
     2521 ttttcagccg ctgttcaaat tcacctttaa cgcccgcgcc agcctgtaac agaccgaggt
     2581 ccagtgtgcg gatatgcacc gttttcaggc tgtccggcac gttgccttcg gtgatacgca
     2641 gcgccagccc ttccaccagc gcggttttgc ccacccccgg ttctcccacc agaatcgggt
     2701 tgtttttgcg gcgacgggag aggatatcga ccatctggcg aatttccgtg tcgcgcccga
     2761 ataccgggtc gattcgcccg ctgcgggctt tttccgtcac gtcttcggta aagcggttaa
     2821 gcaccgccag cagggcgtca tcctgtttcc ccgtcagcgt ttttgccggt gcgtccgtcg
     2881 ttgccgcccc gggttgcgcg gatgtcagcg gttcggtgac ctgtggctga cgtgccggac
     2941 attcatccga ctgcgcatcc agcaggggac gcagccgctc aagctgcaca cggctcaggc
     3001 tcagcaatac ccacaggtca ttacaggcgg gcagcatcgg tttttctgtc agcgccatca
     3061 gcagatgctg gctgcggatt tgtggatcgt cgccttccag tgacgctatc agccacgcct
     3121 gtcggataag cgctgccagc ggttcagaaa gttgaggacg ttcgcggacc gggcggggta
     3181 aggtgtccag atgtgccagc agagactgcc agagcgtgtc gatatcccat tcatagcggc
     3241 gggcaatcac cgtgatatcg ccttctccct gctccagcag tttcagcagc cagtgttcaa
     3301 tggttatttc cgcatgggcg cgggtctgac acagtgaggc ggcagcgtcc agcgcccgtg
     3361 cacagtatgg gttaaggcga cgcagcagcg cggcggggtg atttccggtc atagaaggtt
     3421 tccttatcgt gttctgtatt cctgcgatcc gcgttcaggc acgctaccgc ccatagccgt
     3481 tacctcactc aggcccatca ggtttcacca gattgtgggt atgctttgct cagtaccggt
     3541 gcctgcggca ctggacaaag cacacaacaa aaaggcagac agtttcctgt ctgccgcagt
     3601 tatcaggcgg aaggacgctc attccacgag tcggaatgaa tgatgttgcc gtctttgtaa
     3661 gtccaggtga ttttctcgta gcggaactca atacgttcca ggtggttatg cttctctctg
     3721 gaaggatcct tgatgtcgtg cataagcgga gcgactctga ccagcttgac gttatcaagc
     3781 gtgatgttga agtactccac ttcctgaccg gcatcgttga tgcggtaaaa cttaaattct
     3841 gccgttttca gagtctggcc ggtggtcaca gctttgtaga gatacgggct ggaggcatcg
     3901 atttctttgg taaacgtaaa gggcttgtga gtacgggtac cggtcagttt gccggtgttg
     3961 ttgtcggtcg ggatgtacac atcgtgatcc agcgccacca cttcgatgct accttcgcgc
     4021 ccctgaacgt ccacggaacc tttgatatcc gcgccgccgt catctttcag ccagagataa
     4081 gcaggaatag ccatgagaat tactctcctt gttagtgtga ttgaagcacc gccttcttcc
     4141 ggcggtatgt tttatttcac gtcctgacag gcgtcagaac gcggaacaag actgatttcg
     4201 acacgccggt tgactgcccg gccctgtggc gtgtcgttgg tcgccgcagg ctggctctcg
     4261 cccagtccct gtacggcaaa acaggtggcc gggatgtcgc tggtctgcag catccagttg
     4321 cgcaccgctt cggcacgccg cagcgataac tgctgattgc ttttttcatc gccggtggca
     4381 tcggtatatc cggccacgag gatcagccag cccggttttg cccggatgtt caccagcgca
     4441 tccaccagca cttttgtcga gccgtctttc aggcgggcct gtccgacgtc aaacagcgac
     4501 atactgtcaa gacgcacggt ctgaaccgga agcgaagccg tcacgtccat tttttgctca
     4561 ggcggacgcc agtcgcgaat ggcgcgtaat accggctggc ggatgcgttc accggggtat
     4621 aaccccagac cgagacgtag tggttctcct tcccggtaat acccatcgag catgaccgca
     4681 tcgtctttca gcaccgacag gtgacgggct ttggtgataa attcttccgc cgggacggca
     4741 taaaaccggt gcagatcgtc accgacctgc cgtaataact ggcgatttgc cgtggctgac
     4801 aggcacagcg cggcgatacc cgccacggtg gtgacgccca gcatggtcac gcaggcccgt
     4861 cgcagcgggg taaagcccga ctgacgcggc aaccgccgta acagggcatc cgggaatggc
     4921 agcgcatcgt ctgtgccggt gtccgcgata tccggcgtca ggccggttct ggcggtgatc
     4981 cacaactgcc acaggttgtt atccaccgca ggcaacgagg ggaccagccc aacggtcatc
     5041 gccagtggtg gacatttcgc ctccgggccg ttcagttccg ccagcacatt cagatccagc
     5101 cactgcatca ggctttcggc tttcagcagg taacagagtc gctgactgcg ttcctgtgcg
     5161 ccggattgtg caatccactg cgcgggcatg gtggtctctg cggatgttgc tacctgaact
     5221 tcgccgccgg tacagataaa ccacggtgaa ttcgctctct ccggcaacgg cgagccggac
     5281 agcccgctcc agagaagcca gggtacgttt acgcccgccc ggcgacgcac cgtcgccata
     5341 ctgtgagcga accggcgcag ttgtccggtc agaaccgccg catcccggta catgccgggc
     5401 atgacggtat acgccacggc aaactgcgat gcccacgccg ggcgaagggt cagtaatcgc
     5461 tccacctgcg ccacaagctg ttcttcatca gaaacaggca gatacagccc ttctgaaacc
     5521 tgccggaccg ggctgtcagt gaacaggtgc gccgacatat cgccacagac cagcactacc
     5581 ggctgtcggt aggtttccgg cggtagcgat gacggccata cgtcgtccgc tacgacctgt
     5641 ccctttttct tatgccagta cgccatcacc gccgctgtga cggcgacaag aatcactacc
     5701 acggcatact tcacgaccgc agatacagca aagaaaccca gccaggtagt cagcagcaac
     5761 aacagagtcc ataacaaaac cgtctgtttc agcgcattcc tcactcacga ctccctttaa
     5821 cgcagacttt gcggcagcag ttcatccacc agcacgttca gccagtaatg tagcccccac
     5881 cagacaccgg ccagtaccag tgtgagcgtc accagccaga aaaacggcga atgcagcaaa
     5941 gggtgctgac ggggggataa cggcacgttc agtaccgccg tttccggcag cacaccaaat
     6001 ggcgcgacct gcccgttgag cgtcgaaata agctggtcgc gctccggggc tgccggatcc
     6061 tgataacgcc cgcgaaatcc cagcagcagg acacggtgaa agcaggtcag cacggcctgc
     6121 gcgggggcgg gctcctgtag cacctgcttc atccgctcgt acagcagctc accggcctgc
     6181 agagtgttga agaaatgcga ctggagtgga tccttcatcc agatggcctg accgtcgtcc
     6241 atgccgccac ggctcaggac cgtttcgtcc agcagggcgc actgcgcgta agtaatgtgc
     6301 tccacgctct cccggctgaa cccggcatct ttcaggcgtt cgcggaccag ttccacctgc
     6361 cgctggcagt ggcgatacag ctccataccg tgacgcacgg tggttccctg acgcagttcc
     6421 accaccgtca gaaacgtgtc gcgcagcagc gcgtcgatat tgatatccgt ctcttttttc
     6481 atgtgcgcag caccgcaaac agttcaggtt tcaggtcgcc cattgttccc ggcgcataaa
     6541 tcatgcagca cccggactcc agcatcgatt tagccgcgtc actgtgcata tccagcgcaa
     6601 aatactggtt ctccagccgc agtggcagcg ctgccggaac tgaggtaagc ggcaccagtg
     6661 gaacaccatt cagcgccaca ttgataagca gcgtgacgtc atcaggcgca ccgattttgc
     6721 agaccagcgg tagctggtgc agtacctgat gcgcaggcag agaggaacga acggacaggt
     6781 aaaagtcggc ctcttcccgc aggcgcggat cgtgcaggtc tgctttccag cggttgcccg
     6841 gcagggacgc cagatccagc gcgatcaccc gtgacggcag gctggcttcc agcagttcgc
     6901 tgagcaggga gaaaagtggc ggaaagacga tggacggcga ttcgtggaca tagggcggaa
     6961 cggcgctgac gttatgttca agggaaaacg tcagcagcgc accggcaagc ctcgccagct
     7021 cccgccagac cagttcggga tgaaccgccg ggtagcgcag gaaatcactc agtaccggct
     7081 cggcggagtt cagcgcgtta agcagccaga acagggagac atcggcgaca gcgaagtccg
     7141 ccatacgctg gttgctttcc cggcgcatcg ccatcagacg ctggcacttc gcccgtagct
     7201 gcaacagcag cgtgtccaga cgctgtacca gcccgtcatg ggcgttaaac gccagtaacg
     7261 gcggaatata gctctcatcc tgtgtccagt tgccctgaac atcccgcacc agacgcgcca
     7321 gtgggcaggt gatgtaatcc tgattgttat catgagcaaa gcgcagggac agcgcatgac
     7381 gctcaacggc catatcttca ctgtcactgc cgtagatatc ctgcaccttt tgccattcct
     7441 gacgatagcg cagcggtctt tcagtctgct caccctgacc gagattaccg ccgttaccat
     7501 gcgcaagcgg cagcgccagc aggacaatcg cgctgtcgtt cttcagcgcg cgcagatcgc
     7561 aggcgagcgg cagaacgtca ctcacgtctg aatcgatcag cgtgccatca gcaaaacgca
     7621 gacgtacagc ctgcgttttc agctttccgg acgtcagggc atcgcggtca aagagcacat
     7681 tcgcgatccc ccacggatga cgaatacaaa gttgcgcaat gctgtcattg ctgaacgctt
     7741 cccagcgggc ctgctgctga aactgctgag aggacaggaa tgtcccctca gcccacagtg
     7801 gtcgatgaat tttcatatca caccacggcc cggtcaggct ttcgctttcg gcatctggga
     7861 aaccagtgac aggttgatat ccatcccttc aatctggaaa tgcggcacgg caaagagtct
     7921 gacgcggaag aagcccggat tgtcctcaat atcttccacg ataaccttgc cgtcgcgcag
     7981 cgggtgagac gcctgcagtt catcacccgg atcggtcatc tccgtcacca gtgtgcggat
     8041 ccaggtgtta agctccagtt ccagtacgcg gcggtctttg gtggtaccga tattctcgcg
     8101 ctggatgatt ttcaggtaat gcgcaatgcg ggacagcagg aaaatatagg gcaaacgggc
     8161 gttaatacga ctgttggcgg tggcgtcagc ggtatcatac aacgccggtt tctgggcaga
     8221 gttcgctgag aagaagcagg cgtaatcgcg attcttataa taagagagcg gaataaagcc
     8281 aaggttggca aattcaaatt cgcgggtttc cgggatcatc acttcggacg gaattttgac
     8341 ctgattgccg gtgccgagat cgtaaagatg gatcggcaga tcggccactg cgccacccgc
     8401 ctgcggacca cggatctgca cgcaccagcc attattcaca aagctcttca ccatattggc
     8461 ggcaaaggcg aacgaggcgt ttgtccagag gtatttttcg tgatccgggc ctttgacttc
     8521 ttccacatag ttaaagctgc gtaccggcac tgtgtccggc ccatagggca gacggcccag
     8581 cacgcgcggc atcactaatc ccacgtagcg actgtcgtcg gtatcgcgga acgacttcca
     8641 tttgatatat tccgcgcggt caaaatagtt gccgatatct ttaattgcgg cgacttcttc
     8701 catcgagttt ttaaggaaga acttcggtcc gacggaaccg ataaagggca tatgtgatgc
     8761 agcagagact ctggaaatat tacgcagcag ggcaatatcc tgcgggctgt tatcgaattc
     8821 atagctggat attaatgagg ctacaggttc gccacccggc gtatcgtact cctgaatgta
     8881 tgtatggcga tataaaccac tttgaatgat ttccggagaa tcatcaaagt cctgccgcag
     8941 atgttcttta ctgacatcca gcagttcaat tctgacattt ttgcgaaaat cagtgcgctg
     9001 tacgacgaac catgtgccgc gccagagcga ctcgactttc tggaattccg gatggtgcat
     9061 gactgcatca agctgtttac tgattttctg atccagatcg tcgatatgaa aatccagcag
     9121 gcttttgtcg agtttaacca cgtcctcgcc agacttcgcc agacaggaaa gcagaacgtg
     9181 gatagccgcc gtcacgcgct catcgggtga ggcatcggcc agcgtttcgc tgttttgcca
     9241 tgaatcgatt tcctgaatgg aagagaccgg ggttaaatta attttatcaa acagggactg
     9301 ataaacgccg ccggactcag tggtggtgag tgttgcagtt tcagaggtgg catgttcttg
     9361 ttgtacagac atcagcattt ccttttcagt gatcctttct tctccggttt aacctgaata
     9421 cgcgttcggg ttaaacgttc tcctgcggcg caattttcgc cagttcatca cgcagttctg
     9481 aactgagcgt cgggtctttc aggatatttt ccagttcacg gcgaaacgtt gcgttatcca
     9541 gcagattgga tttcaggtca cgtagtaaat tgcgcatagc gagtaataca cgaagttgtg
     9601 gaatattttt tgctacctgt tcgggctcaa aatccttcag gcttttaaat tcaagcgcga
     9661 tattttgttc attaccgttt ccgtttagcg tatcttctac tgttaaatta accgcaggcg
     9721 aaaactcagc catgacgctg ttgaagttgt ttttattgat atcaatcttg tcccgttcgg
     9781 acagcggacg ctgctctttt ccgttactgt aatcgcctac ggcaagaagc ttgaggggga
     9841 gttcaacttt ctttttagca ttgcctgtat gcaggtcaag cttgatattt acacgagcgg
     9901 cgggaacttc attctggaaa ctgtcggcca tatccttttc ctcatcatcc actttttctg
     9961 atggttattg acataattat attcagggga ttatcggatg atttaaataa aaagcaatga
    10021 tgaggaaaat atacaaatga ataatgtatt tttcagggga atagatacag ttgtatcatt
    10081 ttagatattg tttggcctct tgattatttt tcgggagagg gcggcattat ctggtgagag
    10141 aatttggcat gaaacttaaa ataataacag gataagcttc acaaatttgc acttttaaga
    10201 aattgggtag tatgagagcc tattaagata ttcatgaaac ttattgtccg ggcttccaca
    10261 aaaaattatt aatttttcta ctaattaaaa ggttgtgtgt tttttgtaca agaattatat
    10321 aagcgtagcg ccctgttgtg atattgaaat gttaacataa ccaaaataaa ttgatttata
    10381 gaatatataa atgaaattca ttaatggggt atatgtttaa tatataaata agatggatag
    10441 ctttgattga gggatattaa cggtttactc attgtctcag gcttcactga aacgggtaag
    10501 gaggcgtaaa aacgaaaaaa ccgcaagcta atagtcgctt tgcggtttgt cagcattcta
    10561 ataaattggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag
    10621 ctaccaggct gctccacccc gcgtcaccgt actgctttat ttcatcaaat tttgattggt
    10681 tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct
    10741 gctccacccc gcgtcaccgt actgctttac ttcatcaaat tttaattggt tgcgggggcc
    10801 ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc
    10861 gcgtccgtgg atgcgcacta tactctgctc gcgttttcat gcaacctttt ttttaccccg
    10921 atcacggatt tggcatgatt ttgaacaaaa acaaatcact agagcctatt ttttgtgcaa
    10981 aatttgcgtc agggagaggc tgctcatttc attaaacgcg gtggtttgtt atcttcgttg
    11041 cgccttattt tttaacctga agaagagaac aatgaaagga cgttgggtaa agtaccttct
    11101 tatgggcacg gttgtggcaa tgcttgccgc ctgctcttcc aaaccaaccg atcgcggaca
    11161 gcaatataaa gacgggaaat ttacccagcc tttctctctg gtgaaccagc cagatgccgt
    11221 tggcgcgccg attaatgccg gtgattttgc cgagcaaatt aaccatatcc gtaattcgtc
    11281 accgcgtctg tatggcaacc agagtaatgt ttataacgcg gtgcaagagt ggctgcgcgc
    11341 aggcggtgat acccgcaata tgcgccagtt cggcattgat gcctggcaga tggaaggtgt
    11401 cgacaactat ggtaacgtgc agtttacagg ctattacacg ccggtaattc aggcgcgcca
    11461 tacccgtcag ggcgagttcc agtatccgat ttaccgtatg ccgccaaaac gtggtcgtct
    11521 gccgtctcgt gcggagatct acgcaggagc gctgagtgat aaatatattc tcgcttacag
    11581 taactccctg atggataact tcattatgga tgtgcagggt agcgggtata tcgactttgg
    11641 tgatggcagt ccgcttaact ttttcagcta tgcagggaaa aacggtcatg cctatcgcag
    11701 cattggtaag gtgttgatcg accgtggcga agtgaaaaaa gaagatatgt cgatgcaggc
    11761 gattcgtcac tggggcgaaa cacacagtga agctgaggtt cgcgagctgc tggaacagaa
    11821 cccgtctttc gtcttcttta aaccgcaatc ttttgcaccg gtgaaagggg caagtgcggt
    11881 gccgctggtt ggccgtgctt ctgttgcctc cgatcgttca attattccgc caggtactac
    11941 cttgctggca gaagtgccgc tgctggataa taacggcaaa tttaacggtc agtacgaatt
    12001 gcgtctgatg gtggcgctgg atgtcggtgg cgcaatcaaa ggccaacact tcgatatcta
    12061 tcaagggatc gggccggaag ccggacaccg cgcaggttgg tacaaccact atggacgtgt
    12121 ctgggtgctg aaaaccgccc cgggcgcagg taacgtcttt agcggctgat gtgatattct
    12181 gtatgccgtt tttacggata tgacagggtg aggggtaacc tcaccctttt taattctgag
    12241 gtgctatgtc tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc acagcgcgtc
    12301 tgtatggtga aaaagcattg caactgtttg ctgacgcgca tatttgtgtg gttggtatcg
    12361 gtggtgtcgg ttcctgggcg gcggaagcgc tagcgcgcac ggggattggt gcaatcacgc
    12421 ttatcgatat ggatgatgtg tgcgtcacca ataccaatcg gcaaattcac gctctgcgcg
    12481 ataacgtcgg gctggcaaaa gcggaagtca tggcggagcg tattcgccag attaacccgg
    12541 agtgccgtgt aacggtagtg gatgacttcg tgacgccgga taacgtagcg caatatatga
    12601 atgccggtta ttcgtacgtg attgatgcta ttgatagtgt acggcccaaa gcggcgctga
    12661 ttgcttattg tcggcgcaat aaaatcccgc tggtcacgac cggtggcgct ggtgggcaga
    12721 ttgacccgac gcagattcag gttaccgatc tggcgaaaac gattcaggac ccgttggcgg
    12781 cgaagttgcg cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt aaaggtaagc
    12841 tcggcgtgga ttgcgtgttc tctaccgaag cgctggtgta cccgcagtca gacggcaccg
    12901 tctgtgcgat gaaagccacg gcggaagggc caaagcggat ggattgtgcc tcaggatttg
    12961 gcgcggcgac gatggtgacc gccacttttg gctttgttgc ggtttctcat gcgctgaaga
    13021 agatgatggc gaaagcggcg cgtcaggggt aaacaatcag gtcggatgcg acgcgaacgt
    13081 cttatccgac ctggttctgt gcttaaacct gcttcgccgc agcgataatc gcctcgctta
    13141 acgcattcaa cccctggctg cgtgaggcgc taagttgcgc acgtaatccc agctcatcaa
    13201 acaatgccag cggcgactgt gcctgcaatt cggcagcggt tttcccctca acggcagtca
    13261 acaacaccgc caacaggccg cgcacaatgc gcccttcgct gtcgccaaag aaatgcatct
    13321 tgccgttttc agccacggta tatcccagcc agacgcggtt ttcgcatccg gcaatctctt
    13381 tagcctgcgc ttttaactcc tctggcaaag ccggaagctg tttccccagc atgatcaact
    13441 gccgatattt atcttcccat tgcgtcaacg gtgcgaaggt attgcgtaac gtttctgcgg
    13501 ttacggttgt gccgaacgga tgtccggcga attgcgggtt tgtcattaat ccaccaataa
    13561 ttccagcgcg cggtcaacgg cattcaccag cgcatccaca tcactctttg tattatatgg
    13621 cgcaaatgag gcgcgcagtg tgccggttac gcctaattct gccagtagcg gctgagcgca
    13681 atgctgcccg gcgcgcaggg caataccgta ctccgccagc agtgtcacca tatcgctgtg
    13741 atgaactcca gcaaaatcaa aggccagcag gctggaatcc tggcagcgga atgaacgaaa
    13801 gcctggacgt ttcgccagcg cttcttctgc aagcgttgct aagctacggc tccagctttc
    13861 ggcttggttg atatcgtaat ctgtcagcca ttccagcgcc gcgcttaatc ctatgacgcc
    13921 agcgacattt ggcgttcctg cttccagttt ccacggcgca gattgagtcg tgaaaccgtc
    13981 aaaactcact tcgtgaacca ttttgccgcc gcccagccag ggcgacatcg cttccagcag
    14041 ttctggtttg ccatacagca cgccgatgcc tgtcggacca tacagtttgt gacctgaaaa
    14101 agcatagaaa tcaatatcca gttgctgaac atccgcgggg aaatgcaccg ccccctgagc
    14161 accatcaacc atcaccacca tcccggctga atgagcaaaa gtaatcgctc tcgccagatc
    14221 cgggcaaccg ccagtgacgt tcgacatctg gcccaacgcc agaatccgac tacggggagt
    14281 aatcagttct ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg gcaatttcac
    14341 cactttggcc cccgtttgtt gggcgaccat cagccagggg acgaggttgg cgtggtgttc
    14401 tgccacactg acaataattt catcgcccgg ttgcagacgc ggacgggcat agcattgcgc
    14461 caccatgttg atggattcag tggtgccgcg cgtccagacg atatttttgt catccgatgc
    14521 attcagtaat tgtgccactt tttcccgcgc agcttcgtaa cgcgcggtca ggcgttgggc
    14581 ttcggcaaac tggctgcgat ggacgtttcc ggcgctcagg ctataaaact gtcgggaggc
    14641 ttcaaccacg gcttcaggtt taagcgcggt cgcggcgctg tcgagataga cgcccgcatc
    14701 ctgtagcgcg ggaaactggg cgcgaaactg cgcgggatta aaaacgttca tggtactcct
    14761 cggcttgatg caccgatcgt gacgcaaaat tcactttgat acaaggagag tgctaaccat
    14821 cagaattatc tggatatcct ggcgaagccg cgtcagtaac atatgctgaa tagtgttagg
    14881 cgttaatttt agcctgttaa tcaatactat taagatttat taatcgcttt aaggagaaga
    14941 tgatgaaaaa gactgccgca attatttctg cctgtatgct gacttttgcc ctgagcgcct
    15001 gttccggttc gaactatgtg atgcacacca atgacggacg taccatcgtc tctgacggca
    15061 aaccacagac tgataacgat accggtatga tttcgtataa agacgctaat ggcaacaaac
    15121 agcagatcaa ccgtaccgac gtgaaagaga tggtcgaact ggatcagtaa ttcgcgatcg
    15181 caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg gacagacagg
    15241 gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat cgcagaccaa
    15301 ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag aacacgcatt
    15361 ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt tgctggtaga
    15421 agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta cacagtgtaa
    15481 tctaattgtt aaattcattt aacatcaaag tttaaaagcc atgtctaaac gattaccacc
    15541 gctaaatgcc ttacgagttt ttgatgccgc agcacgccat ttaagtttca ctcgcgcagc
    15601 agaagagctt tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc ttgaggattt
    15661 tctggggcta aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg aggaagggca
    15721 aagctatttc ctcgatatca aagagatatt ttcgcaatta accgaagcga cgcgtaaact
    15781 ccaggcccgt agcgctaagg gggcgttgac ggtcagttta ctccccagtt tcgccattca
    15841 ttggttggtt ccgcgacttt ccagctttaa ttcagcttat ccgggaattg atgttcgaat
    15901 ccaggcggtt gatcgtcagg aagataagct ggcggatgat gttgatgtgg cgatatttta
    15961 tggtcggggc aactggccgg ggctacgggt ggaaaaactg tacgccgaat atttattgcc
    16021 ggtgtgctcg ccgctactgc tgactggcga aaaccccttg aagaccccgg aagatctggc
    16081 taaacatacg ttattacatg atgcgtcacg ccgtgactgg cagacatata cccgacagtt
    16141 ggggttaaat catatcaacg ttcagcaagg gccaattttt agccatagcg ccatggtgct
    16201 gcaagcggct atccacgggc agggagtggc gctggcaaat aacgtgatgg cgcaatctga
    16261 aattgaagcc ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta aaaacgcttt
    16321 ttatctggtt tgtcatgaca gccaggcaga actgggtaaa atagccgcct ttcgccaatg
    16381 gatcctggcg aaagccgctg ctgaacaaga aaaattccgc tttcgttatg aacaataatt
    16441 tacgtagggt acgaccatga ccagccgttt tatgctgatt ttcgccgcca ttagcggctt
    16501 catttttgtg gctctgggcg cttttggcgc gcatgtgtta agtaaaacca tgggggccgt
    16561 tgagatgggc tggatccaga ccggcctcga ataccaggcg tttcatacgc tggcgatctt
    16621 aggtctggcg gtggcaatgc agcgtcgcat cagtatctgg ttttactgga gtagcgtttt
    16681 cctcgcgtta ggcacggtgc tgttcagcgg cagcctttat tgcctggcgc tgtcccatct
    16741 gcgtttgtgg gcgtttgtta caccggttgg cggcgtgagc ttccttgcgg gttgggcgtt
    16801 aatgttagtt ggtgctatcc gtttaaagcg caagggcgta agtcatgaat aaggttgtat
    16861 tgctgtgccg tccgggcttt gaaaaagagt gcgccgcaga aattaccgat aaagccggcc
    16921 agcgggaaat tttcggtttt gcccgcgtga aagagaatgc gggttatgtg atttatgaat
    16981 gttatcaacc tgatgatggc gataagttaa tccgtgagct gccgttcagt tcattaattt
    17041 ttgcccgcca gtggtttgta gtgggggaac tcctgcagca tttgccgcca gaagatcgta
    17101 ttacccccat tgttggcatg ttgcagggtg tagtagagaa gggcggtgaa ctgcgtgttg
    17161 aagttgccga taccaacgaa agcaaagagt tgctgaaatt ctgccgtaaa ttcaccgtgc
    17221 cgctacgcgc tgccttgcgt gatgcggggg tgctggcgaa ctatgaaacg ccgaagcgtc
    17281 cggttgtgca tgtattcttt attgcaccag gctgttgcta taccggttac tcatacagca
    17341 acaataattc gccgttctat atgggcattc cgcgcctgaa atttccggca gatgcgccga
    17401 gtcgttccac gctcaaactg gaagaggcat ttcatgtgtt tattcccgcg gatgagtggg
    17461 atgaacgcct ggcgaacggg atgtgggcgg tggatttggg cgcttgccca ggcggctgga
    17521 cctaccaact ggtgaagcgc aacatgtggg tttattccgt cgacaacggc ccgatggcgc
    17581 aaagtctgat ggataccggg caggtgacgt ggctgcggga agacggtttc aaattccgtc
    17641 cgacacgcag caatatctcc tggatggtat gcgatatggt tgaaaaaccg gcgaaagttg
    17701 cggcattaat ggcgcagtgg ctggttaatg gctggtgccg tgaaactatc ttcaatctca
    17761 aactgccgat gaaaaaacgc tacgaagaag tgtcacacaa tctggcgtat attcaggcac
    17821 agcttgatga acatggcata aatgctcaga ttcaggcgcg gcagttgtat cacgatcgcg
    17881 aagaagtgac ggtgcacgtc cgccgtatct gggctgcggt gggtggtcgt cgcgacgagc
    17941 gataacaggc aaggccgggg cgtcgtctcc ggcctgctac cctttccgtc aggctgttac
    18001 caaagaagtt gcaacctggc gctcatcaga aataatgtgc gtgacctcat ccagatgccc
    18061 gatgcgggct tcccctgaac gattaaattt acttttatca atcaataaca acgattgcgc
    18121 ggcacgtttt aataacattg atttgtaatc agcgttgatg gcattggagt cccacagtgc
    18181 gccgctgcta tcgatccctt cacaagaaaa aataaacaga tcgatttcca gcgatttaag
    18241 ttgggaaatc agcgagggat tgacgtaaca gccatatttg cgctcaagtg tgccgccgga
    18301 actgatcagt tgaatgcgtt cgcgtttacc gagttcatgg caaatcggat ggctattggt
    18361 gaagacctgg atgttgatgt caggcaactg gcgtgccaga taccagcagg ttgaactggc
    18421 gtctaaggct atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg cgatatctgc
    18481 tttatgcgca taatggcttt tcagccggat gtgaaaggga tcgccactgt cttgattttg
    18541 acggtggata tatttagcgc gcccatgatt gcgcagaatt ttaccctgcg tctgtaattc
    18601 attgagatcg cgacgaatgg tttctttact gacctttagc tgttcagaga gagcttccgt
    18661 ggtcaggctg gtatggttca gcagcaggtc gactatcgct tgctggcgtg ccgctttcat
    18721 atttttcacc ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg tatgagatta
    18781 cggtgttacc ccttttttta aaagaatatt cccgtacttc gctcgttcgc ctgtgataac
    18841 gatcgcaaag gctttttgcg cccgttcata aaacgcaaaa cgattgatgc ggatgatgtc
    18901 aggacacggg gcttgtagtg aaagcgcatt acggtaacgt cgttctactt caggatcgag
    18961 agtgtcacct tctaccgccg ccatcatcac cagcggtggt gcataactgt ccagttcaaa
    19021 taacgggata atcgcctgga gcaagtcgct caccaacagg ccatcagcgc ggatcacctg
    19081 cggtcccatc gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat ctccatgccc
    19141 catctctgcc agcactttca atagttcggg agaaattaac ggcgaaattg ttttcagcat
    19201 ttcacacctc ctctataaat tcaggttcag tttgcgggta gaaataacgg tactgataat
    19261 gaatctgcgc gcgggcttct tccgggctgt taaattcccc tacgccatac caaccaaaca
    19321 gcgcagctcc tgcgacggtc gtttcggcat cgtcgagaac ttttaccgga atatcaagca
    19381 tattggcttt aatctgattc cacaatgtgt tgcgacttcc tccgccgact aacaataatt
    19441 cagaggcttt aaagtgcccg attttttcca gcatctgtag attgcgctgt aattgcgtgg
    19501 ttaacccttc cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc gttacgcctt
    19561 gccagccagc gttctggcac gacaataaat cacactgcat tttcactcca tctgcgccag
    19621 gcgcgatcag gcgagcttct tcaatcaaca tttgccaggg agtttcagcc gtccagaaca
    19681 gttttctcac ccattccagc acgccggatg ccagccattg catacctggg ttatacaact
    19741 ctgcctggct atccagttcg caggtagaac cggcgtactg acttaacagc gaagtatcaa
    19801 cctgggcgct gcggaccatt aaaatttccc atgtaccgga agagagcacg ggttcatttt
    19861 gttcggcacc agcgccaaaa agggcgaact gggtatcgtg acctgcggaa atcaccggta
    19921 tgccaacggg taagccgagc attgctgcgg cgctgttctg tagcgtacca atctgttcac
    19981 ccgcttccac cagacgaggg aagagtcggc gtggaatact ggtggcttgt aaaatttgcg
    20041 gactgaaatc gcgttgctgg atatccagca tctggctggt tccggccatc gtaatatcag
    20101 tagtgaattc gccggttaaa cggtggttaa tcagcgacga aataaagagc caggcgtgcg
    20161 cgcgttccag cagttgtgga tgattttctt tcaaccacac caacttatat aacgtattga
    20221 aactaaaggc tccgactcca gaaatagcct gcaactgctg tgcggagatc aaccgttcaa
    20281 tattgtccat taccacggct gttcgcggac atttccagct aatgatcgga tagagcagat
    20341 tgccttgctt atctaccaga gcgccatcca caccaaaggt ggtgacggcg ataccacgga
    20401 tgtggcaatc agtcagttca ctattgattt gccgacagca atcagcaaag cgttgcaaaa
    20461 tggcgtctaa agaccactgg tgccaggtgt tgttttccat cgcgatatcg ctggcattag
    20521 gcgttgaggc gcgggcaaca atttttccct gccgattaac cgcgatggcc ctgacattgg
    20581 tcgcgccaca gtcgagtacc aggataactt cttgtttcat aatcgctcct gcaatatagc
    20641 cggataacat tgcttatccg gctaaccact cttgcttgtt caggcccggt agccggagcg
    20701 accgggcatc acatcaggga gtcatgtatt aacgcttgta caacgggccg tagttctggc
    20761 aagcgcggta atcctggcct tcaatatcca tgccgtgcgc ggcccaggca gaaggacgat
    20821 acactttggt ctcttcaacg ttgtgcatac ataccgggat acgcagcatg gaggcaagag
    20881 tgataaagtc tgcgccaacg tggccgatgg tcagaactcc gtggttagcg ccccagttcg
    20941 ccattaccga gtacacatcc gtaaacggcc ctttaccggt gaggcgcggt gcaaaccagg
    21001 tggttggcca ggttgagttg gtgcgtttgt tgaggatgtc atgcacatcc ttcggcaatt
    21061 ccacgctcca gccttccgcg atttgcagta ccggtcccag gcctttgatg atgttgacac
    21121 gagtcatggt gaacgggacg ccgccttcgg taaggaagcg ggaagagtaa ccgccgccac
    21181 ggaagtattc gtggatcgcc gggcaccatt cggtagcggc gaggcaagcg tcagcctctt
    21241 gctgagagat ttcccagtgc ggcttcatcg tcggtttacc ttcgctgtcg cgttgtttac
    21301 aggagccgtc cagcgcagca gaaccggagt tgatcaaatg gatgatgccg tgttctgcca
    21361 gcccgtccag tttatgcccc gttacacgct caattgcttc tggtgaccag taggtacgga
    21421 catcggcaaa tacctgagcg gtgccggtga gctggtggcc cattaacatt gccacgccgt
    21481 taagactgtc gttttcggtc gccacgacaa acggtttgcg cacgccattc cagtcaaatg
    21541 aactgttgag gatcgcttca gcggtatcgc cattgggata ttgatcggtc cagtgacgtt
    21601 gcccctggaa gcctgcagcg atggcgttgt agccaagtga ttcttccacg cgaccaatat
    21661 cggccagttt gctattgcct tgcatcatgt cgcggataca catcgccatc agtaaacttt
    21721 cgcgcagaac tgcgcggctt tgctcggcat tgcgttgata ctgtttgtta ttttcatctt
    21781 cgccatagcg gaagttttta tcagcccagg ccagcgccat ttccagttcg gcttcgtcgt
    21841 aaatcttctg atcgatacgg cgacgcagtt cggtcatatc caccgcctgg actttcattc
    21901 ccagccagga ttcaaagaag ttgtgatcaa cgatggaacc ggcgataccc atcgaaacgc
    21961 cgcccagcga cagataactt ttacctttca tgctggcgac tgctaaaccg gcgcgggcaa
    22021 agcgcagcag tttttcttca acatcggcag gaatcgatgt gtcatcggca tcctgaacgt
    22081 catgaccgta aatggagaat gccgggatgc ctttctggct gtgagctgcc agagccgctg
    22141 ccaggtaaac agcgccggga cgttcagtgc cgttaaagcc ccaaatggct ttcgggcggg
    22201 ttggatccat gtcgatggtt tcactgccat agcaccagca aggcgttacc gtaatggtga
    22261 ggcctacatt ctgactgctg aatttttctt cgcaagcagc ggcttcagcc atacccgcga
    22321 tacaggtatc ggaaatgaca cactcgacgg cagctccgca ggcatggcgc agtttctcgg
    22381 tcagcagtgc agccgtggct ttcgccatat tcattgtttg ttcttcaagc gactcacgaa
    22441 cacccatgcg acgaccgtca ataaccgggc ggataccaat tttcggtaag ctgatttttt
    22501 tcattactga ttcctcactt tattcggaaa atatgttcag ttagttgccg tttgagaacg
    22561 gaaacgggca aagataaaga tgactgcgaa gcagagtgcc gggatcagtt cggcagtggg
    22621 gatgttgccc gctgcgtcac tgacaaaccc catgaccgga gtgacaatac cgccgccaat
    22681 aatggtcata acgatgaagg acgaaccgta tttggtgtcc tggccgagat tcttaatgcc
    22741 cagcgagaag attgttgggt actgaatcga cataaaggca ctgcataaag tcagggctat
    22801 taagcccaca tgaccgccag cgaaggctga gatcaggcac agtgccatag cgattaatgc
    22861 gtaggctgcc aggactttgt gtggcgcgaa gcgactgatg agccaggtac cggtgaaacg
    22921 accaataaag aagcacacca tggttccggt taaatagtta gcggcaaagc ctgctgtcat
    22981 acctggaatt tcttctacag cgtagcgaat taaatagctc cagcaggccg tttgtgcgcc
    23041 aacgtagcag aattgcgcta atactgccca gcgccagtgg cgaatacgcg ccaggcgaga
    23101 aagcgatgcg gagaacgatc cttgtttggc gtcactgtga ttatcactct gcaatgccgg
    23161 gaatttcgtc agcatgatca gcagggcgac cagtaacacg atagccacga tgatcatata
    23221 aggtgtctgt accgataata ccaagctgtg tttatacgca ctcaattgct ctggagacat
    23281 tttatcgaga acgtcttgcg attgatgtgg cacgttagac aaaataagac tttgcccaaa
    23341 gacaaccgcg ataattgcgc caaatgagtt aaatgtttgc gcaagattta agcggaagtg
    23401 accactgctt tccggcccta ataccgtaac aaaagggttt gcggcagttt ccagacaacc
    23461 taatccggct gcaataataa ataggccaac taaaaacaag gtgtagttca ttatttctgc
    23521 ggcgggccag aataatgcag cgccgaaggc atataaaaac aatccggtaa taatccctgc
    23581 tttataactg agttttttca tcaatatccc ggcagggatt gggataatga aataaccaaa
    23641 gtaaaaggcc gattggatca ggccagcctg gaaatttgtc agcgtaaaag cctgctggaa
    23701 ttgaggcaat aaaatgtcgt taaggttatt ggctaccgcc caaagaaaaa acagtgagca
    23761 cagcagcgcg aatggaataa tgtaacttct gctttgccct gcatctttat ctaccgcacg
    23821 gtaactctgc gtttgtattg atgtgtttcc catagcatcc tcttaggttc agaagcttaa
    23881 gcacgtaatc ggttatgttt tcaaaagtgc aaaaccagcg agttcgggct tcatatcagt
    23941 gaatatttgc ctgtctgaaa tgaactcatg gattcatttt caaataaaaa acgctctgta
    24001 atatgacggc ggtcacactt attagccgga gggtgacttt atgtgactac catcacttta
    24061 ataatcgaaa tttcatgccc gtttcagaaa ataaatcata aaaaacacaa accagatgac
    24121 cgtatt
//
LOCUS       DAOFSV010000059        23961 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.059, whole genome shotgun sequence.
ACCESSION   DAOFSV010000059 DAOFSV010000000
VERSION     DAOFSV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.059"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            81..605
                     /gene="tnpA"
                     /locus_tag="QBH27_002491"
     CDS             81..605
                     /gene="tnpA"
                     /locus_tag="QBH27_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDQ7014791.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(762..1559)
                     /locus_tag="QBH27_002492"
     CDS             complement(762..1559)
                     /locus_tag="QBH27_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7014792.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLCYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1569..2120)
                     /gene="ybcL"
                     /locus_tag="QBH27_002493"
     CDS             complement(1569..2120)
                     /gene="ybcL"
                     /locus_tag="QBH27_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDQ7014793.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(2289..2621)
                     /gene="emrE"
                     /locus_tag="QBH27_002494"
     CDS             complement(2289..2621)
                     /gene="emrE"
                     /locus_tag="QBH27_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDQ7014794.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2965..3279)
                     /gene="fliE"
                     /locus_tag="QBH27_002495"
     CDS             complement(2965..3279)
                     /gene="fliE"
                     /locus_tag="QBH27_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDQ7014795.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3494..5152
                     /gene="fliF"
                     /locus_tag="QBH27_002496"
     CDS             3494..5152
                     /gene="fliF"
                     /locus_tag="QBH27_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDQ7014796.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEMEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            5145..6140
                     /gene="fliG"
                     /locus_tag="QBH27_002497"
     CDS             5145..6140
                     /gene="fliG"
                     /locus_tag="QBH27_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDQ7014797.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            6133..6819
                     /gene="fliH"
                     /locus_tag="QBH27_002498"
     CDS             6133..6819
                     /gene="fliH"
                     /locus_tag="QBH27_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDQ7014798.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6819..8192
                     /gene="fliI"
                     /locus_tag="QBH27_002499"
     CDS             6819..8192
                     /gene="fliI"
                     /locus_tag="QBH27_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDQ7014799.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSKTHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            8211..8654
                     /gene="fliJ"
                     /locus_tag="QBH27_002500"
     CDS             8211..8654
                     /gene="fliJ"
                     /locus_tag="QBH27_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDQ7014800.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8651..9778
                     /gene="fliK"
                     /locus_tag="QBH27_002501"
     CDS             8651..9778
                     /gene="fliK"
                     /locus_tag="QBH27_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDQ7014801.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLTGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9883..10347
                     /gene="fliL"
                     /locus_tag="QBH27_002502"
     CDS             9883..10347
                     /gene="fliL"
                     /locus_tag="QBH27_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDQ7014802.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10352..11356
                     /gene="fliM"
                     /locus_tag="QBH27_002503"
     CDS             10352..11356
                     /gene="fliM"
                     /locus_tag="QBH27_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDQ7014803.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11353..11766
                     /gene="fliN"
                     /locus_tag="QBH27_002504"
     CDS             11353..11766
                     /gene="fliN"
                     /locus_tag="QBH27_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDQ7014804.1"
                     /translation="MSDMNNPADDNNGAMDDMWAEALSEQKSTSGKSAADAMFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11769..12134
                     /gene="fliO"
                     /locus_tag="QBH27_002505"
     CDS             11769..12134
                     /gene="fliO"
                     /locus_tag="QBH27_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDQ7014805.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            12134..12871
                     /gene="fliP"
                     /locus_tag="QBH27_002506"
     CDS             12134..12871
                     /gene="fliP"
                     /locus_tag="QBH27_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDQ7014806.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12881..13150
                     /gene="fliQ"
                     /locus_tag="QBH27_002507"
     CDS             12881..13150
                     /gene="fliQ"
                     /locus_tag="QBH27_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDQ7014807.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            13159..13944
                     /gene="fliR"
                     /locus_tag="QBH27_002508"
     CDS             13159..13944
                     /gene="fliR"
                     /locus_tag="QBH27_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDQ7014808.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            14234..14857
                     /gene="rcsA"
                     /locus_tag="QBH27_002509"
     CDS             14234..14857
                     /gene="rcsA"
                     /locus_tag="QBH27_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDQ7014809.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKDTTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14901..15089)
                     /gene="dsrB"
                     /locus_tag="QBH27_002510"
     CDS             complement(14901..15089)
                     /gene="dsrB"
                     /locus_tag="QBH27_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDQ7014810.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            15252..15479
                     /gene="yodD"
                     /locus_tag="QBH27_002511"
     CDS             15252..15479
                     /gene="yodD"
                     /locus_tag="QBH27_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDQ7014811.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15775..16590
                     /locus_tag="QBH27_002512"
     CDS             15775..16590
                     /locus_tag="QBH27_002512"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDQ7014812.1"
                     /translation="MFSIQQPLLVFSDLDSTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWITATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16587..18281)
                     /gene="yedQ"
                     /locus_tag="QBH27_002513"
     CDS             complement(16587..18281)
                     /gene="yedQ"
                     /locus_tag="QBH27_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDQ7014813.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLKNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(18452..18634)
                     /locus_tag="QBH27_002514"
     CDS             complement(18452..18634)
                     /locus_tag="QBH27_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDQ7014814.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18713..19630)
                     /locus_tag="QBH27_002515"
     CDS             complement(18713..19630)
                     /locus_tag="QBH27_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDQ7014815.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19803..20723
                     /gene="yedA"
                     /locus_tag="QBH27_002516"
     CDS             19803..20723
                     /gene="yedA"
                     /locus_tag="QBH27_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDQ7014816.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20712..21182)
                     /gene="vsr"
                     /locus_tag="QBH27_002517"
     CDS             complement(20712..21182)
                     /gene="vsr"
                     /locus_tag="QBH27_002517"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDQ7014817.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(21163..22581)
                     /gene="dcm"
                     /locus_tag="QBH27_002518"
     CDS             complement(21163..22581)
                     /gene="dcm"
                     /locus_tag="QBH27_002518"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDQ7014818.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22648..23343)
                     /locus_tag="QBH27_002519"
     CDS             complement(22648..23343)
                     /locus_tag="QBH27_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDQ7014819.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVTLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(23383..23748)
                     /locus_tag="QBH27_002520"
     CDS             complement(23383..23748)
                     /locus_tag="QBH27_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDQ7014820.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         5942 a         6221 c         5987 g         5811 t
ORIGIN      
        1 cacgctcctg ttgaccacct ggtcttaacc ggaatttgaa tgcctgtagt cgtttcatgg
       61 gccaaatcat actttggcct atgaaaaaag aaaccgatat ccgacgcgga aggcattgtg
      121 ttttcctgaa gcatgttcac ctggtcttcg ttactaaata ccgacgtcag atttttgacc
      181 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
      241 aacgggttga aatggatggc ggaccagatc acgtccattt gttaattaac tatcctccca
      301 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgagtagg ttactgcgac
      361 gagatcgacc agatattgca gtcaggtatt acgacaaagg cgttttgtgg agtcctggct
      421 attttgtcag tagctgcatc ttaacgtatc aactagattt aataacaacg atatccgtac
      481 ctaaccggat atctaatgtc ataagcatcc ctttaattat cccctcaacc tcttgtaact
      541 ttcccactct tgagctccac atagtccaaa cgccggatgc aaaaaaatca cacgtgcatt
      601 tctgaaattg tgatggagga agatagtatc aattatatgt gcttagaaac gtccccgatt
      661 agcgcagaag tcaccggatt gttcagactc caataggttg agtatgccgt atttattttc
      721 caaaataaaa atcgatgctg ttgctccttc ataaaatact tttactctct ggacgctttt
      781 cttagctctc ttggtgttat cttaaaacga ttatggaacc tttcagtaaa acgagaagga
      841 cacttgtaac cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca
      901 gacaacgcat tatttaacct cacgtcgtcc aatatacttt gaaaacttac tccctcgctt
      961 gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac cttgtttgct
     1021 gtccattttg ttccaggttc ggatagcatt aggttataac aacgacttat taatgattgt
     1081 ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag tagaacgccc
     1141 attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt ttttagttgc
     1201 tcccaacaat atattaactc ggggttctga ggtaagcaga aacctgtcgt gttacgaatt
     1261 tgatcagtta tcgcataaaa tttttgaaac ttttcaatta gatcaatggg ataacaaagc
     1321 atttctgcaa gataaagacc tgcttctgga taattcgcaa tataaaattc atatccacag
     1381 ggaaataata ttatctgatt attatcaaca gttagactat gcgtctccca attgataact
     1441 ttttttccct gacggatacg acataaagct ggcataagag gcttaaccct gtgaatctca
     1501 tgatgtttat gcatccgtat ttcttcaatc tttaggttag tcttatcttt tgccagcata
     1561 ctcttaccct actttatctc ataaactggt gttatctcag cggttgcaat tttattggca
     1621 ttaagcatat aaccaactaa cgctccgctg gaattagaat ccacaggaat tttatcagtt
     1681 tttagagccc atactttaaa ctggtaatga tggggtttat ctcctttggg aggacatgct
     1741 ccaccaaatc ctgcataacc aaaatcattt cggccttgaa cagcaccagt cggaagtttt
     1801 gtaccatcac gtcttcctgc atcagcgggc aaatatgtta cagttgctgg aatattagca
     1861 acagtccagt gccaccatcc actgcctgta ggagcatctg gatcatacac agttacggca
     1921 aagcttttgg tgccttcagg agcaccagac caggttaatg agggcgatgt attaccacct
     1981 tcacacccaa atccagaaaa tacatgagac gttgtaagtt gctctcctgt ttttatttca
     2041 ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg ttatgaatgc caatacactt
     2101 gaaacgataa gttttttcat aaaaacctca ttgttgtgac ctatcgttat tttatttgat
     2161 gtgccttgat ctcattatgc ataaaggcgc aatgatcgcg caaatgcaat cataattata
     2221 aaggtgcccc gaacattagc cataatatgc acaaatcaga ttcctgttat ctcaaggata
     2281 attatttttt aatgcggtgc acttcgtgac aacaagttaa tgactaacac accggcacaa
     2341 atcaacatca tgcctatgat agctggcagg tccagtcgtt gaccaaaaaa tccccaggaa
     2401 agcaaactaa tcagaacgat accgactcct gaccagatag cataagcaat ccctgtaggt
     2461 atataagcca gcgtctgagc taataaccag aatgatgcac aatagcaaat aattgtgcca
     2521 acagagggcc ataaccgtgt aaacccctct gaaaacttca ttaaggttgt accaatgacc
     2581 tctgcaagta ttgcgccacc aagataaata taagggttca tagcatattc tttcctgttc
     2641 atactggaga gaattgtact acagtttgaa cacaactcac tgttttcatc ctggtgtatg
     2701 tgtatccagg gggctctggc gtggcaagag ataaaggcag gaacaataat gatcagaagg
     2761 aaccccaggc taacgaaaga gttcactctt cctgggagca aagcgaagac aaaacatgtt
     2821 atcgcgttta cggatcgtgg atggcagaaa acaaccagga tcaggtatcc attttaaacc
     2881 agaaattgag tgagtttgcc catccatgcc ccacgctgtg ggatcggata taattaaaca
     2941 agcatagtca acaggttaca caacctacac ctgcatggac atcacttcct gatacgccgc
     3001 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
     3061 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     3121 gcgggcggct gtttgtgtat cgcttatgcg atcgagcgcg gcgtgcagct gcccggcaaa
     3181 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     3241 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     3301 tgatagcaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     3361 gggtttattg gcggatagaa aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga
     3421 gtttgctgac ccgggagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc
     3481 cacgaggtgc gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg
     3541 cctgcgtgcg aatccgaaaa ttccattgat tgttgccggt tccgcagcag tggcggtcat
     3601 ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc
     3661 cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt
     3721 cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa gttcacgaac tgcgcctgcg
     3781 cctggcgcaa caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga
     3841 aaagtttggt atcagccagt tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga
     3901 gctttctcgt accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat
     3961 gccgaaaccg tctttattcg tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa
     4021 tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc gctattgtac atctggtttc
     4081 cagcgcggtt gctggtctgc cgccaggaaa cgtcacgctg gtggatcagg gcgggcatct
     4141 gttaacccag tccaatacca gcgggcgcga tcttaatgac gctcagttga aatatgccag
     4201 cgatgtcgaa ggccgtattc agcggcgtat tgaagcgatc ctgtcgccta ttgttggtaa
     4261 cggtaatatt cacgcccagg tcacggcgca actggacttc gccagtaaag aacaaacgga
     4321 agaacagtat cgccctaacg gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa
     4381 tgagagcgag caaagcggtt ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca
     4441 accggcacct gcgaataacg caccaatcag cacgcctccg gcaaatcaaa ataaccgcca
     4501 gcagcaggcg agcaccacca gcaatagtgg gccgcgtagc acacagcgga atgaaaccag
     4561 taactacgaa gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg
     4621 tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca gacggtaaac cattgcctct
     4681 cagcaacgaa cagatgaagc aaattgaagc tctgacccgc gaggcgatgg gcttttctga
     4741 aaaacgcggt gattcgctca atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg
     4801 cggagcactg ccgttctggc aacagcaagt gtttatcgat cagttgctgg ctgccggtcg
     4861 ctggttgctg gtactgctgg tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct
     4921 aacacgtcgc gccgaggcgg tgaagactgt gcagcaacaa gcgctggccc gcgaggaaat
     4981 ggaagatgcg gtggaagtcc gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa
     5041 ccaacgactg ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc
     5101 gcgcgtggtg gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag
     5161 gcaccgataa aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt
     5221 tcaagcacct ctcccagcgc gaagtgcaaa cccttagcgc tgcaatggcg aacgtcacgc
     5281 agatctccaa caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt
     5341 ttgccgcact gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg
     5401 gtgaagaacg tgccgccagc ctgctggaag atattctcga aacccgcgat accgccagcg
     5461 gtattgaaac gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc
     5521 atccgcaaat tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc
     5581 tggcgttgtt cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg
     5641 gcgtgcagcc agccgcgctg gcggaactga ccgaagtact gaatggcttg ctcgacggtc
     5701 agaatctcaa gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga
     5761 tgaaaacgca gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg
     5821 cgcagaaaat catcgacgag atgttcctgt tcgagaatct ggtggatgtc gatgatcgca
     5881 gcattcagcg tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg
     5941 ccgagcagcc actgcgcgag aaattcttgc gcaatatgtc gcagcgtgcc gccgatattc
     6001 tgcgcgacga tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga
     6061 aagcgattct gctgattgtg cgccgtcttg ccgaaactgg cgagatggtg attggcagcg
     6121 gcgaggatac ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg
     6181 ccaccaccgg cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc
     6241 gaacccagcc ttgagcagca actggcgcaa ctgcaaatgc aggcccacga gcagggttat
     6301 caggcaggga ttgccgaagg tcgccagcaa ggtcatgaac agggctatca ggaaggactg
     6361 gcccaggggc tggaacaagg tctggcagag gcgaaggctc aacaagcgcc aattcatgcc
     6421 cggatgcagc aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgata
     6481 gcttcgcgcc tgatgcagat ggcgctggag gcagcacgtc aggtcatagg tcagacgcca
     6541 acggtggata actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta
     6601 ttcagcggta aaccacagct gcgcgtacac ccggatgatc tgcaacgtgt tgatgatatg
     6661 ctcggcgcaa ctttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccatcct
     6721 ggcggttgca aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg
     6781 caagaactct gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct
     6841 ggctaaccac gctagataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct
     6901 acgggcgatt aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc
     6961 tcggcgcaac ctgtgtcatt gagcgccaga acggcagcaa aacgcacgaa gtagaaagcg
     7021 aagtcgtcgg ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtggaaggtg
     7081 tcctgcccgg cgcgcgtgta tatgccaaaa acatttcggc agaagggctg caaagcggca
     7141 agcagttgcc gctcggtccg gcgttattag gtcgtgttct ggacggcagc ggtaaaccgc
     7201 tcgatggcct cccctccccc gatacgacgg aaaccggtgc gctgattacc ccgccattta
     7261 acccattgca acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg
     7321 ccctgcttac cgtcgggcgc gggcagcgta tggggctgtt tgccgggtcc ggcgttggta
     7381 aaagtgtgct gttggggatg atggcacgtt acacccgcgc cgatgtcatt gtcgtgggtt
     7441 tgattggtga acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag
     7501 gacgtgcacg ctcagtggtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc
     7561 aaggtgccgc ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt
     7621 tgctgattat ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga
     7681 ttggcgaacc acctgctact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac
     7741 tggtcgagcg tgccggaaat ggcattagcg gcggcggctc gattaccgcg ttttataccg
     7801 tgctcaccga gggcgatgac cagcaggacc ccattgccga ctccgcgcgg gccatcctcg
     7861 acggtcacat tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata
     7921 ttgaagcgtc gatcagccgc gcaatgacgg cgttgatcag tgagcaacat tacgcacgag
     7981 tgcgcacctt caaacagctg ttgtcgagtt ttcagcgtaa ccgagatctg gttagcgtcg
     8041 gcgcgtatgc caaaggcagc gatccgatgc tcgacaaagc catcgccctg tggccgcagc
     8101 tggagggcta tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg
     8161 ggctggagcg tattttcccg acagtgtcat aacccaggag ataacggcag atggcagaac
     8221 atggcgcgct ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc
     8281 tgctgggtga aatgcgtcgc ggatgtcagc aggcggaaga acagctcaaa atgctgatcg
     8341 attatcagaa tgaatatcgc aataacctca atagcgatat gagtgccggg atgaccagca
     8401 accgctggat caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc
     8461 gccagcaact taatcagtgg acgcagaaag ttgacattgc cctgaacagc tggcgagaaa
     8521 agaaacaacg tttgcaggcc tggcagacac tgcaggaacg gcaatccacg gcggcactgc
     8581 ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga
     8641 ggaaacctga atgattcgct tagcgccctt aattaccgcc gacgttgaca ccaccacatt
     8701 gcctggcggc aaagccagcg atgctgcaca agattttctc gcgttgttga gcgaagcatt
     8761 agcaggcgag acaactaccg acaaagcggc cccccagttg ctggtggcaa cagataagcc
     8821 cacgacaaaa ggcgagccgc tgatcagcga tattgtttcc gacgcgcaac aagcggattt
     8881 actgatcccc gtggatgaaa cactgcctgt catcaacgac gaacaatcca catcaacacc
     8941 gttaaccacc gctcagacga tgacgttggc tgcggtggct gacaaaaata cgacaaaaga
     9001 cgaaaaagcg gatgatctta atgaagacgt caccgcaagc ctgagcgccc tttttgcgat
     9061 gttgccgggt tttgacaata cgcccaaagt gactgatgca ccgtcaaccg tgttaccggc
     9121 agagaaacca acgcttttca caaaactgac ttctgcgcaa ctcacaacag cacagcctga
     9181 tgacgccccc ggcacaccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa
     9241 agcggaagtc atcagcacac cttcgccggt gactgctgcc gccagcccgc taatcactcc
     9301 acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc
     9361 tcacgaatgg caacaatcat taagccagca tatttcgctg ttcacccgcc aggggcaaca
     9421 aagtgcagag ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt ccctcaaagt
     9481 ggatgataac caggcgcaga tccagatgat ttcaccgcat cagcacgtac gcgcagccct
     9541 ggaagcagcg ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca
     9601 aagcaacatt agtggcgaaa gctttagtgg tcagcagcag gccgcttccc agcaacagca
     9661 aagccaacgc acagcaaacc atgaacctct gacgggggaa gacgacgata cgcttccggt
     9721 tcccgtctct ttacaagggc gtgtaacagg caacagcggc gttgatattt tcgcctaacg
     9781 tcagaggtag caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat
     9841 aattagccga taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata
     9901 agcaagaaaa gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc
     9961 tgtgccagcg caggttacag ctactggcat tcgcatcagg ttgccgctga cgacaaagcg
    10021 cagcaacgcg tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatttg
    10081 ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc gactgaaaga tgaagcaacc
    10141 cgctcgcggc tgagtgagta tttgccggaa gtccgtagtc gcttgctgtt actgttttca
    10201 cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt
    10261 aaaaccacac tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg
    10321 ctgtataccg cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga
    10381 aattgatgcg ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtgttag
    10441 cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg
    10501 tttgcaggcg ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt
    10561 caacctgctg cgccgtagcc cggacattac cgtcggggcc atccgcattc agccgtacca
    10621 tgaatttgcc cgcaacttgc cggtgccgac caacctgaac cttatccacc ttaaaccgct
    10681 gcgcggcaca gggctggtgg tgttctcgcc gagcctggtg tttatcgccg tggataacct
    10741 gtttggcggc gatggacgct tcccgaccaa agtggaaggt cgcgagttta ctcataccga
    10801 acagcgcgtc atcaaccgca tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa
    10861 agcgattaat ccgctggaag ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa
    10921 tatcaccacc tcgccgaacg acattgtggt taacacgccg ttccatgtgg agattggcaa
    10981 cctgaccggc gaatttaata tctgcctgcc attcagcatg atcgagccgc tacgggaatt
    11041 gttggttaac ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct
    11101 ggtgcgccag gtgcagcatt cacagctgga actggtggcc aactttgccg atatctcgct
    11161 acgcctgtcg cagattttaa aactgaaacc cggcgacgtc ctgccgatag aaaaacccga
    11221 tcgcatcatc gcccatgttg acggcgtccc ggttctgacc agtcagtacg gcactctcaa
    11281 cggtcagtat gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga
    11341 ggaacagccc aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac
    11401 gatatgtggg ctgaagcgtt gagcgagcaa aaatcaacca gcggaaaaag cgctgccgac
    11461 gcaatgttcc agcaatttgg cggcggagat gtcagcggaa cgttgcagga tatcgacctg
    11521 attatggata ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa
    11581 gagctgttgc gtctgacgca aggatcagtc gtggcgctgg acggtctggc gggcgaaccg
    11641 ctggatattc tgatcaacgg ttatttaatc gcccagggcg aagtggtggt tgttgccgat
    11701 aaatatggcg tgcggatcac cgatatcatt accccgtccg agcggatgcg ccgcctgagc
    11761 cgttagtgat gaataaccat gctactgtgc aatcttccgc gccggtttct gctgcgccac
    11821 tgctgcaggt gagcggcgca ctcatcgcca ttattgccct gatcctcgct gccgcctggc
    11881 tggtaaaacg gctgggattt gcccctaaac gcactggcgt taacggtctg aaaattagcg
    11941 ccagtgcttc gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa gatgcacggc
    12001 tggtgctcgg cgttaccgca gggcaaatca atctgctaca taaacttccc ccttcagcac
    12061 caacggagga gataccgcag accgattttc agtcggtcat gaaaaatttg cttaagcgtg
    12121 gcgggagatc ctgatgcgtc gtttgttttc tgtcgcaccg gtccttctct ggctgattac
    12181 gcccctcgcc ttcgcgcaac tgccgggtat caccagccag ccgctacctg gcggtggaca
    12241 aagctggtcg ctcccggtgc agacgctggt gttcatcacc tcgttgacgt tcattccagc
    12301 gattttactg atgatgacca gtttcacccg catcattatt gtttttggtt tattgcgtaa
    12361 cgcgctggga acgccctccg cgccacctaa ccaggtattg ctgggactgg cactgttttt
    12421 gacctttttt attatgtcgc cagtgatcga caaaatttat gtcgatgcgt accagccatt
    12481 cagcgaagag aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga
    12541 gtttatgctg cgtcagaccc gcgaggcgga tttagggttg tttgccagac tggcgaatac
    12601 cggcccgttg caggggcctg aagctgtgcc gatgcgcatt ttgctcccgg cctacgtgac
    12661 cagcgagttg aaaaccgcat ttcagatagg cttcacgatt ttcatccctt ttttgattat
    12721 cgacctggtg attgccagcg tgttgatggc attggggatg atgatggttc ccccagccac
    12781 cattgctctg ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt
    12841 cggttcgctg gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat
    12901 gatgatgggg actgaagcga tgaaagtcgc gctggcactg gctgccccac tgttgctggt
    12961 ggcgctggtc acgggtctta tcatcagtat tttgcaggcc gccacgcaga tcaacgaaat
    13021 gacgctgtcg tttattccaa aaatcatcgc cgtatttatc gccattatta ttgccggacc
    13081 gtggatgctc aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat
    13141 catcgggtag gccgtacaat gatgcaggta acaagcgatc aatggctttc ctggttaagc
    13201 ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc
    13261 gaacgcagcg tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgccatt
    13321 gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc
    13381 gtgcagcaga tcctgatcgg cattgcgctt ggttttacca tgcaatttgc ctttgccgct
    13441 gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacatttgtc
    13501 gatcctggca gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    13561 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    13621 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc actcaccaaa
    13681 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    13741 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    13801 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    13861 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    13921 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    13981 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    14041 gctcactcac atatcgcaac atttacttta ctttaagaca attccaggca aattatacaa
    14101 cactttacgg gatagtaagt ccgcctgaaa aattgcgaga gtggcgcatt aggtgaccca
    14161 tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga
    14221 gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt
    14281 aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt
    14341 tgatgacctt gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga
    14401 ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag cacatcatta atcaacatcc
    14461 caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt
    14521 ggtcagaaaa aatttattga tcagctctaa atcgattaaa ccggaatctc tcgacgatat
    14581 ccttggcgat attctgaaaa aagatacaac gataacctcg tttttaaata tgccgacttt
    14641 atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat
    14701 tcaaatctcc gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat
    14761 taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga
    14821 taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc
    14881 accagtcagg ctgaataaga ttactctgct ttctcaacaa agataccgtc ctgatgccct
    14941 gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg
    15001 ccttcactaa actcctcaac tgccagtacc acgccaggac gacgcggacc gccatccgtt
    15061 ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta
    15121 gaacaatttc gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc
    15181 tatacttaag agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc
    15241 gaggaaagat gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg
    15301 tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca
    15361 gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat
    15421 tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa
    15481 tgcacaataa aaaaatcccg accctgaggg ggtcgggatg aaacttgctt aagcaagaag
    15541 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ctgcaatttt
    15601 tttcgctgac aagaaaatat tcgcgctatg aatgattaac gctacttatg aatagaaatg
    15661 tgacgcgcgt caccccttgg tggctctggt gtcaacggga tcgctcccga aaaaatcatc
    15721 tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcccatgttt
    15781 tcaattcaac aaccactact ggtttttagc gatcttgata gcaccctgct ggacagtcat
    15841 agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc
    15901 gtcattctct gtagcagtaa aacttcagcg gaaatgctgt acttgcaaaa aatgttgggg
    15961 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
    16021 caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag
    16081 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
    16141 gatgcaacca ttgccgaatg gacgggatta agccgtagcc aggcggcgct gacgcagctg
    16201 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
    16261 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
    16321 gatgcctctg ccggaaaaga tcaggctgcc aactggatta ccgcgaccta tcaacaattg
    16381 tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
    16441 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt ccatctgcat
    16501 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
    16561 gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattacgc
    16621 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
    16681 taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta
    16741 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
    16801 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
    16861 ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
    16921 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
    16981 tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt
    17041 tcgaacaatg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
    17101 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagatg
    17161 agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
    17221 tcgccgcgca cgccttcgct tagcgtatgg acgcgcacca gcacaccgtc gaaatggtcc
    17281 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
    17341 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
    17401 ggatttgaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
    17461 tttttatcga tggcgttttt taaaaattgc tgcatagtac gcacgggaat actcatcccc
    17521 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
    17581 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
    17641 cgttgtgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
    17701 ttgcgcgtaa acaaggtcgg cagcgtcgaa acgtaaaatc cagcacgcga gacatacacc
    17761 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
    17821 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggagatt cccctcgctg
    17881 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
    17941 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
    18001 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
    18061 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctggctg
    18121 gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
    18181 aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt
    18241 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
    18301 cttttttatg attctggcat aacccggcga tagcgtaccg caaaacagcg ataattttat
    18361 aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga
    18421 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    18481 cccccggaac aagctcggtt tcatgaaaag cttcccgctt gaccccgtac ccgtcatacc
    18541 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
    18601 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    18661 ttgttcacct tagttgaaga tggcgaattt tgcataatag cctgtgcgag tattaatgtg
    18721 cctgaccgcg cattttcgct acggctttca ctcccagcac cacgatgccg ccgatgataa
    18781 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    18841 cggcgaaatg ttcaatggcg tgatgcaacg gcgcaatacc atgtaccaca atcccgccgc
    18901 cgacgaggaa catcgccagc gtgccgacaa tcgataacgc tttcatcaac cagggcgcaa
    18961 tgatcaataa ccctttacct aatgcctgca tcagcgcgct ggatttttcc gccagccaat
    19021 accccaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
    19081 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcttct gccacaatcc
    19141 ccagcgtgat ggcgacgatt tccgcagaca atataaaatc ggtacgaatc gcccctttta
    19201 ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctgac
    19261 tctgcgccgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    19321 cttcaaagca gagaaacgcg ccgccaatca tcaacagtgg cgtaatcgcc cacgggataa
    19381 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gaacctttcg
    19441 ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
    19501 tgagcgataa gtcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    19561 cggagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
    19621 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    19681 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    19741 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttcag ccgtgaggga
    19801 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    19861 ggggctcaac ctattttgtc attcggattg gcgtggaaag ctggcctccg ttaatgatgg
    19921 cgggcgttcg attcctggca gccggtattt tattgctggc atttttgcta ctgcgcggac
    19981 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    20041 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    20101 ccgtagtggt tgcaaccgtg ccccttttta ccctgtgctt cagccgcctg tttggcatta
    20161 aaacgcgcaa actggaatgg gtgggtatcg ccattgggct tgccggaatc atcatgctca
    20221 atagcggtgg aaacttaagc ggcaatccat ggggcgcgat tctgatttta atcggctcga
    20281 ttagctgggc gtttggctcg gtttatggct cgcgcattac cttacctgta gggatgatgg
    20341 cgggtgcgat tgagatgctg gcggctggcg tggtgttaat gattgcgtcg atgattgcgg
    20401 gtgaaaaact gacggcgctc ccttcccttt caggcttcct tgcggtcggc tatctggcgc
    20461 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgcaac gtcagtccgg
    20521 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    20581 tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gtcttcgcgg
    20641 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    20701 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    20761 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtaag cttctcgcgc
    20821 ccacgtaacg cacactccca gacaatcagt acgcgccagc cgagttcctg caagcgactg
    20881 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcggttcga
    20941 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
    21001 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
    21061 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    21121 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    21181 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    21241 tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat aggcctgagt
    21301 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    21361 gcattcacga ggtgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
    21421 tttctcaccc gtggccatat cccagccgcg gtcgattaaa atttccgcgc catccttgta
    21481 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
    21541 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    21601 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
    21661 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
    21721 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
    21781 gtcgatgatt ttcggatcgt ccggcccgtt atctgctgcg tcggcaacgt catagcccag
    21841 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
    21901 tttgacattt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    21961 aaagaacagc gtaccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    22021 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    22081 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    22141 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
    22201 gttggcttta taagtgcgta cagcatgttt gttccattcg ctggtaaaca cgcactgtcc
    22261 gccaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg
    22321 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
    22381 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    22441 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata
    22501 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    22561 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    22621 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
    22681 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca taaactcgac
    22741 taaaaattgc gcgttgtgct gtgccagctg cttgcctctt gcggtttgca tggtttgcgg
    22801 taatttcagt aactttgtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg
    22861 atgctgtgcg aacggatctt cgccatcaaa cagcgccaag cccaatgccc ctgaaacggc
    22921 aaatacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat
    22981 tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg
    23041 acaaacggcc tcaattttct ccgccggaaa ttgcgcgaac tcttcacgga gcaggcgacg
    23101 cgtctcttct gccgccagga ttgaagaacg ctgccgttgc ggatgatttt tcgccagggt
    23161 cacgatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc
    23221 cgccagtttc tgtgccgtag cccagacacg gcgaaaatga cacaagtcat gcgccgcgtc
    23281 ctgatgttga tgatggtttt tcaaccagtt atcgaactgt gcctgccagt gttgtaactc
    23341 catacgcgct ccgttgccaa agagtcgcaa ccttagcagt ttttattcat agcgtctgca
    23401 acgtgcggga cgaggtcggg tgtaccatgc aatgccgcct aacaaggcac caaccagggc
    23461 cataaacaga aatccgacca atgctcctaa ccacttcccc gcggtagatc ctaaatcact
    23521 ttcaaatccc gacacggggg catcggggat ccggctcatc acccagatgt atcgcgccat
    23581 tgcccagacg aaatagccac accatgtata gaaagcccac aaagccaatt tgccgccagg
    23641 gctgcgagag agtctctctt ccatctttgt cgaaccgtat tccattttga gataacccgg
    23701 aaaattcaga cttagaacta ttatgtgata aaagtcacat ttttccacat tggatgagcc
    23761 agtcaagact caatcatttt ttgaacaaat agataaattt tgcgcaacgg cggtgaatac
    23821 aggaaaaatc gatttgaacg atgttattta tctttgattt cggctttatt aattcattca
    23881 atcaatatat tggtactgat tacaattata cctttttatt tttctgtgtc atcatgcctc
    23941 cgttattagc cttttatcgt c
//
LOCUS       DAOFSV010000060        23474 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.060, whole genome shotgun sequence.
ACCESSION   DAOFSV010000060 DAOFSV010000000
VERSION     DAOFSV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.060"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            214..2886
                     /gene="rcsD"
                     /locus_tag="QBH27_002521"
     CDS             214..2886
                     /gene="rcsD"
                     /locus_tag="QBH27_002521"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDQ7014821.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2903..3553
                     /gene="rcsB"
                     /locus_tag="QBH27_002522"
     CDS             2903..3553
                     /gene="rcsB"
                     /locus_tag="QBH27_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDQ7014822.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3753..6602)
                     /gene="rcsC"
                     /locus_tag="QBH27_002523"
     CDS             complement(3753..6602)
                     /gene="rcsC"
                     /locus_tag="QBH27_002523"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDQ7014823.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            6769..8595
                     /gene="atoS"
                     /locus_tag="QBH27_002524"
     CDS             6769..8595
                     /gene="atoS"
                     /locus_tag="QBH27_002524"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDQ7014824.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8592..9977
                     /gene="atoC"
                     /locus_tag="QBH27_002525"
     CDS             8592..9977
                     /gene="atoC"
                     /locus_tag="QBH27_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDQ7014825.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRVIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            10173..10835
                     /gene="atoD"
                     /locus_tag="QBH27_002526"
     CDS             10173..10835
                     /gene="atoD"
                     /locus_tag="QBH27_002526"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDQ7014826.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10835..11485
                     /locus_tag="QBH27_002527"
     CDS             10835..11485
                     /locus_tag="QBH27_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HDQ7014827.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            11482..12804
                     /locus_tag="QBH27_002528"
     CDS             11482..12804
                     /locus_tag="QBH27_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDQ7014828.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12835..14019
                     /gene="atoB"
                     /locus_tag="QBH27_002529"
     CDS             12835..14019
                     /gene="atoB"
                     /locus_tag="QBH27_002529"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDQ7014829.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(14093..14869)
                     /locus_tag="QBH27_002530"
     CDS             complement(14093..14869)
                     /locus_tag="QBH27_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDQ7014830.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYFGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14874..16523)
                     /locus_tag="QBH27_002531"
     CDS             complement(14874..16523)
                     /locus_tag="QBH27_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDQ7014831.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSVLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     SALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKLGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRHATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(16524..21038)
                     /locus_tag="QBH27_002532"
     CDS             complement(16524..21038)
                     /locus_tag="QBH27_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDQ7014832.1"
                     /translation="MGTGLANAEDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKDST
                     SHSGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNRWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELPPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAP
                     GSEWIGMQVK"
     gene            complement(21062..21685)
                     /locus_tag="QBH27_002533"
     CDS             complement(21062..21685)
                     /locus_tag="QBH27_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDQ7014833.1"
                     /translation="MRHGLLALICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(21682..23370)
                     /locus_tag="QBH27_002534"
     CDS             complement(21682..23370)
                     /locus_tag="QBH27_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDQ7014834.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAVQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
BASE COUNT         5730 a         6218 c         5823 g         5703 t
ORIGIN      
        1 gcaatcttcg tttatttgca attaatttcg ttgggctttt tgtaggttat ttgtacagca
       61 aaatggcgct tgtacatcta tttcccccaa tgccggatga taaatatcac tggagaatag
      121 agaatcatca atcaggtaag agtctggaat ttcacactgt accctttata ctgccctatc
      181 acttcgcgaa gttttaacag gtcataaaca cgaatgcgtc agaaagagac aacggccacg
      241 acccgctttt cactcctacc ggggagcatt acccgcttct ttttactgtt gatcattgtg
      301 ttactggtga cgatgggtgt aatggtacaa agcgccgtta acgcctggct gaaagataaa
      361 agttaccaga ttgtcgacat tacccacgca atccaaaagc gcgtcgatac ctggcgttac
      421 gtgacctggc agatctacga caacattgcc gcgacgacct ccccctcctc cggcgaaggt
      481 ttacaagaga cgcgcctgaa acaggatgtc tactatctgg agaaaccacg ccgcaaaacg
      541 gaagcgttaa tctttggctc tcacgacaac tcaacgcttg agatgactca acggatgtcc
      601 acctatctgg acacattgtg gggcgcagaa aatgtaccgt ggtcgatgta ttacctgaat
      661 ggtcaggata acagtctggt gctgatctca accctgcccc tcaaagatct cacctccgga
      721 tttaaagaat cgaccgtcag cgacattgtt gattcacgtc gtgcagagat gttgcaacag
      781 gccaacgccc tcgatgaacg cgaaagtttt tctaacatgc gccgcctggc ctggcagaac
      841 ggtcattact ttaccttgcg taccacgttc aaccagccgg gacatctggc aacggtcgtg
      901 gcttttgatc tgccgattaa tgatttgatc ccaccgggta tgccgctgga cagtttccgc
      961 cttgagccag acgcgacggc aacgggaaac aatgaaaatg agaaagaagg gacggatagc
     1021 gtcagtatcc actttaacag tacgaagatt gaaatctcct cggcactcaa ctctaccgat
     1081 atgcgcctgg tctggcaggt tccttacggc accttactgc tggatacgtt gcaaaacatt
     1141 ctgctgccac tgctgctgaa catcggtttg ctggcgctgg cgttatttgg ctataccaca
     1201 ttccgccatt tctccagccg cagtacagaa agcgtcccca gcacggcggt caataacgaa
     1261 ttgcgcattt tacgggcaat taatgaagag atagtctcac tgctgccgct cggcctgctg
     1321 gttcacgatc aggaatcaaa ccgcacagtc ataagtaaca aaattgccga tcatttgctg
     1381 ccgcatttga atctgcaaaa catcaccacc atggcggaac agcatcaggg gattattcag
     1441 gcgacgatca ataacgagct gtatgagatc cgcatgttcc gcagccaggt tgcgccgcgc
     1501 acacaaattt tcattattcg cgatcaggat cgcgaagtgc tggtaaacaa gaaactcaag
     1561 caggcgcagc gtctgtatga gaaaaaccag caggggcgga tgacctttat gaaaaacatt
     1621 ggcgatgcgc tgaaagaacc cgcacagtcc ctggcggaga gcgcggctaa actcaacgcc
     1681 ccggaaggca aacaactggc gaatcaggct gatgtgctgg tgcgactggt cgatgaaata
     1741 cagttagcga acatgcttgc ggacgatagc tggaaaagtg agacggagct gttctccgtg
     1801 caggatttaa ttgatgaagt agtgccttca gtgttgcctg ccatcaagcg taaaggtctg
     1861 caactgctga ttaacaatca tctgaaagca cacgatatgc gccgcggcga tcgcgatgcc
     1921 ttacgacgta ttttgctgct actgatgcaa tatgccgtga cctcaacgca attaggaaaa
     1981 atcacccttg aggttgatca ggatgagtcc tccgaagacc gcctgacgtt ccgcattctg
     2041 gacactggag aaggcgtaag cattcatgaa atggataatt tgcacttccc gtttatcaac
     2101 cagacccaaa acgatcgcta tggcaaggcg gacccgctgg cattctggct gagcgatcaa
     2161 ctggcgcgta aactgggcgg tcatttaaac atcaaaacgc gggatgggct tggtacacgc
     2221 tactctgtgc atatcaaaat gctcgcagct gacccggaag ttgaagagga agaagagcga
     2281 ttactggatg atgtctgcgt aatggtggat gttacttcgg cagaaattcg gaatattgtc
     2341 actcgccagt tagaaaattg gggtgcaacc tgtatcacac ccgatgaaag attaattagt
     2401 caagattatg atatcttttt aacggataat ccgtctaatc ttactgcctc tggcttgctt
     2461 ttaagcgatg atgagtctgg cgtacgggaa attgggcctg gtcaattgtg cgtcaatttc
     2521 aatatgagca acgctatgca ggaagcggtc ttacaattaa ttgaagtgca actggcgcag
     2581 gaagaggtga cagaatcgcc tctgggcgga gatgaaaatg cgcaactcca tgccagcggc
     2641 tattatgcgc tctttgtaga cacagtaccg gatgatgtta agaggctata tactgaagca
     2701 gcaaccagcg actttgctgc gttagcccaa acggctcatc gtcttaaagg cgtatttgcc
     2761 atgctaaatc tggtacccgg caagcagtta tgtgaaacgc tggaacatct gattcgtgag
     2821 aaggatgttc caggaataga aaaatacatc agcgacattg acagttatgt caagagcttg
     2881 ctgtagcaag gtagcctatt acatgaacaa tatgaacgta attattgccg atgaccatcc
     2941 gatagtcttg ttcggtattc gcaaatcact tgagcaaatt gagtgggtga atgttgtcgg
     3001 cgaatttgaa gactctacag cactgatcaa caatctgccg aaactggatg cgcatgtgtt
     3061 gattaccgat ctctccatgc ctggcgataa gtacggtgat ggcattacct taatcaagta
     3121 catcaagcgc catttcccaa gcctgtcgat cattgttctg accatgaaca acaacccggc
     3181 gattcttagt gcggtattgg atctggatat cgaagggatc gtgctgaaac aaggtgcacc
     3241 gaccgatttg ccgaaggctc tcgccgcgct gcagaaaggg aagaaattta ccccggaaag
     3301 cgtttctcgc ctgttggaaa aaatcagtgc tggtggttac ggtgacaagc gtctctcgcc
     3361 aaaagagagt gaagttctgc gcctgtttgc ggaaggcttc ctggtgaccg agatcgctaa
     3421 aaagctgaac cgcagtatta aaaccatcag tagccagaag aaatctgcga tgatgaagct
     3481 gggtgtcgag aacgatattg ctctgctgaa ttatctctct tctgtgacgt tgagtccggc
     3541 agataaagac taatcacctg taggccagat aagacgcgtc agtgtcttat ctggcatttg
     3601 cacggattgc cggatgcggc gtaaacgcct tatccgtcct acgattccca ttatttcaac
     3661 agattacatt aaagtaggcc agataagacg cgtcagcgtc gcatctggca tttgcactga
     3721 atgccagatg cggcataaac accttatccg tcctacgatt cccgcgattt cctgaccctc
     3781 tcggcatata ccgtcagcgt ctgttttatc acatccagcg ttaccggctt cgacaggcag
     3841 ctgtccatac cggactccag acaccgctgc ttctcttcag ccaacgcatt agcagttact
     3901 ccgattaccg gcaacgtcag tcccaactga cgaatgcgtt gcgtcaagcg gtaaccatcc
     3961 atatttggca tattgacgtc gctaagcacg atatcaatat gattcttgtt aagtacatta
     4021 agcgcatcga cgccatcatt cgcggtttta cattgatagc ccaacgatcc caactgatct
     4081 gccagcagac gccggttaat cggatgatca tccacgacca gaatcatcat atcgtcatta
     4141 tcgctgaccg ctttgtccgt cgacggcaga gcgttagcag gatcgtcgct ctccatctcg
     4201 atcaaataaa tacgcgccaa caatgccggt agctcatgcg gagcagccac actatgtacc
     4261 cactcccctg gcgctttctc cagcggaata ccaatatggc gacgacagaa ggtcactacc
     4321 gctctgccct gccatttttt gttcactacc tcgtcagtaa ttaacacatc ttcgggagtc
     4381 ggttcctgcc cttcgtatgt tgtaacgacg atgccgctgc gctgcaaact ggtttccagg
     4441 aactgacaga gcgacgcatt gcggaccgcc agccagcagc gtttaccact caacccttcc
     4501 acaccttttt tctgcgggta ctgagcgccg tataacggaa tacgcacggt aaactggctg
     4561 cccattcccg gttctgaatc taccgagatg tcgccgtcca tcatgctgat cagtttttca
     4621 caaatcgcca gacccagacc agtcccctgg aagttacgct gtacgcccgt tccgacctgg
     4681 aagaagggat caaacaagcg caccacttct ttcgccggta tccccacgcc ggtatcgcga
     4741 acacggatag agagataatc gccatcagcg cgaacatgca aaactataca gccggtatcg
     4801 gtgaatttta tggcgttact caacaggttg gagatgacct gctgtaaacg catcgggtcg
     4861 ccatttaagg ccactggcac atccggttca ataaagcagt acaagcctaa ctgcttgcgt
     4921 accaccagcg gtaaatagtt ggcggtgatg tggttcatca cttcacgcgg tgaaaactca
     4981 cgcggttcga tcttcaactg ttctgattca attttcgaga aatcgagaat atcgctgata
     5041 attttcaaca acaggctgga agagttgttc attgccgtca ccagacgatc gacgcctttc
     5101 ggtaactctt tggtttgcaa cagatccagg ttaccgataa tgccatacag cggcgttcgc
     5161 agctcatgac tgacggtggc caggaacatc gattttgact ggctcgcctg ttccgctgct
     5221 tgtgccatct cctgcaacga ctcttccatc ttcacgcgcg aagaaacatc caccagcaca
     5281 caaatggcca cgttttcatt acgatagcgc gaatggacga agctgatttg cagattggta
     5341 ttgttgctgg tcaggacatc aacaaaattg acctgctgcc cacagataat ttgtgtcagc
     5401 cgttggcggt cctcatgcgt aagcatattg agataagtat gcgccagttc gttacttaaa
     5461 atattgacgc catcagcggt acgcaaaatg cagataccca ctggcgcgga ggcgacaatc
     5521 ttgcgattaa actgctcatg ttcttccagt cgcagggcgt cgctttccgc cggaatgaaa
     5581 atacgccgct cgtacatccg cgcgagagta aacaatgcag ctccggcaag cacattcagc
     5641 aaaattgcgt taaggatcaa catgcgaatg cgttccagca ccttatcaac tggcactgaa
     5701 taaacgatgc ttagcgatga gggtggcaga tttttcttca gcaccagttc ccggaaccct
     5761 tccgtatagc caaaccagga gcgttcctgc atccagcgag gatcgccctt aattttgctt
     5821 tctggtccgg taagcgaaat cagggtatga ccattttcat caagaatggt aacccccatc
     5881 ggcaacgtac caggtaagaa aaagttctcc atccggatgg tttgctcgac acccaaaagc
     5941 gcctgcaacc ggttcgccag ataaactggc gtcaaggcgt aaaaataccc gacgccaggg
     6001 cgcggacctt cgctgatcca gtagaggtta ctgccactat catcttgtgg tgcatttcga
     6061 tatttattaa tgcgttcatg caaagctttt agcgcggtat cgcgttccac tggcatatca
     6121 cgcagaccga aattggccat gcagaggtta tcgctgccga ttaaaaatac ccggtttaga
     6181 tcgtaagccg cagaaaaatt atcgcgccag tagcgcataa accacgccaa tgactccaga
     6241 gaacctcgcc aggtgttact cattgcggaa caatcagagt cagcaaacag cggttcaaac
     6301 gcaggcacat ccgtctgcgt ttctcgtcca cgcggggaaa gcacaccgtt ttctgccgat
     6361 aagcgatttt cagctatgta cttcagctct ttcatcacat cagaggtgcg ttgaataaag
     6421 cgctgagcct gatcggaact cagattaaat tcctgacgaa tttccgattc tcgctgatgt
     6481 aacgcattaa cgatgtaaaa aacggatgaa aaagcaatca acagccagag cactaacgcc
     6541 aatgctctga acatgtagcg cgaggctttc agggttgtac gaaaagaagc aaggtatttc
     6601 aaaggggcga agctccgcct caggtgaccg atggagtgtg gttaaggtag cggtaaaagc
     6661 gtgttaccgc aatgttctct cttctctgga atatgataca ccgccgagaa atcatcacct
     6721 taacctctga taatcgtcat ataccggaca agactagtgg atttcagcat gcattatatg
     6781 aagtggattt atccacgccg cttacgcaat caaatgatcc tgatggcaat cctgatggtc
     6841 attgtcccaa cgcttactat tggttatatc gtagaaacgg aaggacgttc agcagtctta
     6901 tctgaaaaag agaaaaaact ttctgccgtg gtcaacctgc ttaatcaggc gctaggcaat
     6961 cgctacgatc tctacatcga cttaccgcgt gaggagcgta tccgcgcatt aaatgcagaa
     7021 cttgccccca ttaccgaaaa tatcactcac gccttccctg gcatcggcgc tggttattac
     7081 aacaaaacgc tggatgcgat aatcacctac gcgccttcag cgctatatca gaataatgtg
     7141 ggcgttacca ttgccgcaga tcaccctggt cgcgaagtca tgcgtacaaa tacccctttg
     7201 gtttattcag gcaggcaggt gcgcggcgat attttgaatt caatgattcc cattgagcgt
     7261 aatggtgaaa tcctcggcta tatctgggcc aatgaattaa ccgaagatat tcgccgccag
     7321 gcctggaaaa tggatgtgag gattatcatt gtgctcaccg ccggtttgct gataagcctg
     7381 ctgttgattg tccttttctc ccgtcgcctg agcgccaata tagatatcat caccgatggc
     7441 ctctcgactc tggctcaaaa tattcccact cgattaccac aattgcccgg tgaaatgggg
     7501 caaatcagtc agagtgttaa caacctcgcc caggcactgc gtgaaacgcg aacacttaac
     7561 gatctgatta ttgaaaacgc tgccgatggc gtcattgcca ttgaccgcca gggtgatgta
     7621 accaccatga acccagcagc agaagtcatc actggctatc aacgtcatga actggtaggg
     7681 cagccttact ccatgttgtt cgacaatact cagttctaca gtcccgtact ggatacgctg
     7741 gaacatggca ccgaacatgt tgcactggag atcagttttc caggccgtga ccgcaccatt
     7801 gaactcagtg tcacgaccag tcgtattcat aacacgcacg gtgaaatgat aggtgctttg
     7861 gtgattttct ctgatttaac tgcccgcaaa gaaacccagc gccgcatggc gcaagcagaa
     7921 cgcctcgcca cactgggtga gctgatggct ggcgtggcgc atgaagtacg taatccgcta
     7981 acggctattc gtggttatgt acagatcttg cgccaacaaa cccgtgaccc aatacatcag
     8041 gaatatctgt ccgtagtact caaagaaatc gattcaatta acaaagttat tcagcaattg
     8101 ctcgaatttt cacgcccacg ccacagtcaa tggcaacaag tcagcctcaa tgcattggtt
     8161 gaagaaactc tggtactggt acaaaccgcc ggcgtacaag cgcgggtcga cttcataagc
     8221 gaactggata atgaattaag cccgattaac gccgatcgtg aactgctcaa acaggtacta
     8281 ctgaatatcc tgatcaatgc cgtccaggct atcagtgcac gagggaaaat tcgcattcga
     8341 acctggcagt atagcgactc gcaacaggcc atttcgatag aggacaacgg cagtggcatt
     8401 gatctctcgc tgcaaaaaaa gatcttcgat ccctttttca ctaccaaagc ctcaggaacc
     8461 gggcttggtc tggcgttaag tcaacgcatc attaatgccc atcagggtga tattcgcgtc
     8521 gccagtttgc cgggctacgg cgcaaccttc acgcttattt taccgatcaa cccgcaggga
     8581 aatcagactg tatgactgct attaatcgca tccttattgt ggatgatgaa gataatgttc
     8641 gccgtatgct gagcaccgct tttgcactac aaggattcga aacacattgt gcgaacaacg
     8701 ggcgcacagc attacacctg tttgccgata ttcaccctga cgtggtgttg atggatatcc
     8761 gcatgccaga gatggacggc atcaaggcac taaaggagat gcgcagccat gagacccgga
     8821 cacccgttat tctgatgacg gcctatgctg aagtggaaac cgccgtcgaa gcactacgct
     8881 gcggagcctt cgactatgtt atcaaaccgt ttgatctcga tgagttgaat ttaatcgttc
     8941 agcgcgcttt acaactccag tcaatgaaaa aagagatccg tcatctgcac caggcactga
     9001 gcaccagctg gcaatggggg cacattctca ccaacagccc ggcgatgatg gacatctgca
     9061 aagacaccgc caaaattgcc ctttctcagg ccagcgtctt gattagcggt gaaagcggca
     9121 ccgggaaaga gttgattgcc agagcgattc actacaattc gcggcgggca aaggggccgt
     9181 tcattaaagt caactgcgcg gcactgccgg aatcgttgct cgaaagtgaa ctgtttggtc
     9241 atgaaaaagg tgcatttact ggtgcacaaa ccttgcgtca gggattattt gaacgtgcca
     9301 acgaaggtac tctgctcctc gacgaaattg gcgaaatgcc gctggtactg caagccaaat
     9361 tactacgcat tctgcaggaa cgggaatttg aacggattgg cggccatcag accataaaag
     9421 ttgatatccg cgtcattgct gccaccaacc gcgacttgca ggcaatggtg aaagaaggca
     9481 ccttccgtga agatctcttt tatcgcctta acgttattca tttaatactg ccgcctctgc
     9541 gcgatcgccg ggaagatatt tccctgttag ctaatcactt tttgcaaaaa ttcagtagtg
     9601 agaatcagcg cgatattatc gacatcgatc cgatggcaat gtcactgctt accgcctggt
     9661 catggccggg aaatattcga gagctttcca acgttattga acgcgccgtc gtgatgaatt
     9721 caggcccgat cattttttct gaggatcttc cgccgcagat tcgtcagcca gtctgtaatg
     9781 ctggtgaagc aaaaacagcc cctgtcggtg agcgtaattt aaaagaggaa attaaacgcg
     9841 tcgaaaaacg catcattatg gaagtgctgg aacaacaaga aggaaaccga acccgcactg
     9901 ctttaatgct gggcatcagt cgccgtgcat tgatgtataa actccaggaa tacggtatcg
     9961 atccggcgga tgtataatgc caaaacttgc tatgcagaaa tttgcacagt gcgcaatttt
    10021 ctgcatagcc gctcattctc cttatgaacc cccatctaat ttatcctttt ctattcaatt
    10081 agttaaataa caacattcaa taatctcatt ctggcactcc ccttgctatt ccctgactgt
    10141 acccacaacg gtgtatgcaa gagggataaa aaatgaaaac aaaattgatg acattacaag
    10201 acgccaccgg cttctttcgt gacggcatga ccatcatggt gggcggattt atggggattg
    10261 gcactccatc ccgcctggtt gaagcattac tggaatctgg tgttcgcgac ctgacattga
    10321 tagccaatga taccgcgttt gttgataccg gcatcggtcc gctcatcgtc aatggtcgag
    10381 tccgcaaagt gattgcttca catatcggca ccaacccgga aacaggtcgg cgcatgatat
    10441 ctggtgagat ggacgtcgtt ctggtgccgc aaggtacgct aatcgagcaa attcgctgtg
    10501 gcggagctgg acttggtggt tttctcaccc caactggtgt cggcaccgtc gtagaggaag
    10561 gcaaacagac actgacactc gacggtaaaa cctggctgct cgaacgcccg ctgcgcgccg
    10621 acctggcgct aattcgcgct catcgttgcg acacacttgg caacctgacc tatcacctta
    10681 gcgcccgcaa ctttaacccc ctgatagccc ttgcggctga tatcacgctg gtagagccgg
    10741 atgaactggt cgaaaccggc gagctgcaac ctgaccatat tgtcacccct ggtgccgtta
    10801 tcgaccacat catcgtttca caggagagca aataatggat gcgaaacaac gtattgcgcg
    10861 ccgtgtggcg caagagcttc gtgatggtga catcgttaac ttagggatcg gtttacccac
    10921 aatggtcgcc aattatttac cggagggtat tcatatcact ctgcaatcgg aaaacggctt
    10981 cctcggttta ggcccggtca caacagcgca tccagatctg gtgaatgctg gcggacaacc
    11041 gtgcggcatt ttacccggtg cagccatgtt tgatagcgcc atgtcatttg cgctaatccg
    11101 tggcggtcat attgatgcct gcgtgctcgg cggtttgcaa gtagacgaag aagcaaacct
    11161 cgcgaactgg gtagtgcctg ggaaaatggt gcccggtatg ggtggcgcga tggatctggt
    11221 gaccgggtca cgcaaagtga tcatcgccat ggaacattgc gccaaagatg gttcagcaaa
    11281 aattttgcgc cgctgcacca tgccactcac tgcgcaacat gcggtgcata tgctggttac
    11341 tgaactggct gtctttcgtt ttattgacgg caaaatgtgg ctcaccgaaa ttgccgacgg
    11401 gtgtgattta gccaccgtgc gtgccaaaac agaagctcgg tttgaagtag ctgccgatct
    11461 gaatacgcaa cggggtgatt tatgattggt cgcatatcgc gttttatgac gcgttttgtc
    11521 agccggtggc ttcccgatcc actgatcttt gccatgttgc tgacattgct aacattcgtg
    11581 atcgcgcttt ggttaacacc acaaacgccg atcagcatgg tgaaaatgtg gggtgacggt
    11641 ttctggaact tgctggcgtt tggtatgcag atggcgctta tcatcgttac cggtcatgcc
    11701 cttgccagct ccgctccggt gaaaagtttg ctgcgtactg ccgcctccgc cgcaaagacg
    11761 cccgtacagg gcgtcatgct ggttactttc ttcggttcag tcgcttgtgt catcaactgg
    11821 ggatttggtt tggttgtcgg tgcaatgttc gcccgtgaag tcgcccggcg agtacccggt
    11881 tctgattatc cgttgctcat tgcctgcgcc tacattggtt ttctcacctg gggtggcggc
    11941 ttctctggct caatgcctct gttggctgca acaccgggca acccggttga gcatatcgct
    12001 gggctgatcc cggtgggcga tactctgttc agtggtttta atattttcat cactgtggcg
    12061 ttgattgtgg tgatgccatt tatcacccgc atgatgatgc caaaaccgtc tgacgtggtg
    12121 agtatcgatc cgaaactact catggaagag gctgattttc aaaagcaact accgaaagat
    12181 gctccaccgt ccgagcgact ggaagaaagc cgcattctga cgttgatcat cggcgcactc
    12241 ggtatcgctt acctggcgat gtacttcagc gaacatggct tcaacatcac catcaatacc
    12301 gtcaacctga tgtttatgat tgcgggtctg ctgctacata aaacgccaat ggcttatatg
    12361 cgtgctatca gcgcggcagc acgcagtacc gccggtattc tggtgcaatt ccccttctac
    12421 gcggggatcc aactgatgat ggagcactcc ggtctgggcg gactcattac cgaattcttc
    12481 atcaatgttg cgaacaaaga caccttcccg gtcatgacct tttttagttc tgcactgatt
    12541 aacttcgccg ttccgtctgg cggcgggcac tgggttattc aggggccgtt cgtgatgccc
    12601 gcagcccagg cgctgggcgc tgatctcggt aaatcggtaa tggcgatcgc ctacggcgag
    12661 caatggatga acatggcaca accgttctgg gcgctgccag cactggcaat cgccggactc
    12721 ggtgtccgcg acatcatggg ctattgcatc actgccctgc tcttctccgg cgtcattttc
    12781 gtcattggtt taacgctgtt ctgacggcaa ccctacagac agaaggaata taaaatgaaa
    12841 aattgtgtca tcgtcagtgc ggtacgtact gctatcggta gttttaacgg ttcactcgct
    12901 tccaccagcg ccatcgacct gggggcgaca gtaattaaag ccgccattga acgtgcaaaa
    12961 atcgattcac tacacgttga tgaagtgatt atgggtaacg tgttgcaagc cggactgggg
    13021 caaaatccgg cacgtcaggc gctgctaaaa agcggactag ctgaaacggt gtgcggattc
    13081 acggtcaaca aagtgtgcgg ttcaggtctg aaaagcgtgg cgcttgctgc acaggcgatt
    13141 caggcaggtc aggcacagag cattgtggcg gggggtatgg aaaatatgag tttagcgccc
    13201 tacttactcg atgcaaaagc acgctctggt tatcgtcttg gagacggaca ggtttatgac
    13261 gtaatcctgc gcgatggcct gatgtgcgcc acccatggtt atcatatggg gattaccgcc
    13321 gaaaacgtgg ctaaagagta cggaattacc cgtgaaatgc aggatgaact ggcgctacat
    13381 tcacagcgta aagcggcagc cgcaattgag tccggtgctt ttacagccga aatcgtcccg
    13441 gtaaatgttg tcacccggaa gaaaaccttc gtcttcagtc aagacgaatt cccgaaagcg
    13501 gattctacgg ctgaagcgtt aggcgcattg cgcccggcct tcgataaagc aggaacagtc
    13561 accgccggga acgcgtcagg tattaacgac ggtgctgccg ctctggtgat tatggaagaa
    13621 tctgcggcgc tggcagcagg cctgaatccc ctggcacgca ttaaaagtta tgccagcggt
    13681 ggcgtgcccc ccgcattgat gggtatgggg ccagtacctg ctacgcaaaa agcgttacaa
    13741 ctggcggggc tgcaactggc ggatattgat ctcattgagg ctaatgaagc atttgctgca
    13801 cagttccttg ccgttgggaa aaccctgggc tttgatcctg agaaagtgaa tgtcaacggc
    13861 ggggccatcg cgctcggaca tcctatcggt gccagtggtg ctcgtattct ggtcacacta
    13921 ttacatgcaa tgcaggcacg cgataaaacg ctggggctgg caacactatg cattggtggc
    13981 ggccagggaa ttgcgatggt gattgagcgg ttgaattaac caataaaaac gcccgatagc
    14041 gaaagttatc gggcgttttc ttgaacatcg acggcgaagg ttaccccatt aatcaccagt
    14101 caaaactttt caccagcgtc agctcgccag cattacgcat cggtacaata aaagtttcct
    14161 gtttctcatt gaccgaccct tcatcggtga tcagcgtcag ttgggccgta gttaattccg
    14221 tttcactgcg cccgccgaag tagttgatat acacctgata acggccgtga actggcgctg
    14281 gcatggcgaa aatctcgggt ccgtaccctg tcgtgacatc catatccagt gcgccactgt
    14341 ttttcagcac tgtgttaccg taccaggcat gttcaccgtc aggcgtaacg acatgaagat
    14401 cgaggtcggt attgtccgta tcccaggaga gaaccagccg taaacgcgcg cgaatcgccc
    14461 cagcacccgg cgttgagtaa aactgcattt tttgtcggct ttgcccatct gggctgatga
    14521 cctgcacgct gttgctgcct tcagtgaaaa tataagggcg tgcaaaagaa ccatctgatt
    14581 caattctctg cggcatactg gcaccgttga ccaccagccg gccctgctga actttacccg
    14641 ccgcagcata attctttatt ttgccgcgga tctgcgctga gatattttga tcatctgcca
    14701 tgttgacgga cgacgccggg taattgatcg tttgtgaaaa actggcatct tcgccctctg
    14761 ccggatgcca gccagagagc ggtgcatcaa tctcgacttc ctgcacacct tcactgtgag
    14821 cgacaggcga aagcgccacc agtaacaacg gaagaaaaat ctttcgcata gcattagtcc
    14881 attaataatt gtcgggtcag cgtttcgata tagttctcat caagcccggt gggataacca
    14941 tcaaaggcca ggtgcagata ctcatgggtt aaatccagcc gatcctgaag cgtaaagaaa
    15001 ttacgaataa acagccgttt ctgctgccga tcggtatagg ggaaacctga gactaaacga
    15061 cagaccgcaa acacgtctgg ttcgttgtat cccgtctcac cttgtaatat acgccgccac
    15121 tgcggcattt ttttcgccag ccaggctttc gctttgggta ataactggca ggttgagcgt
    15181 ggcgctcccc agcggctaag gctgttgtcg ggataagcaa acgcgagtat ctgatcataa
    15241 cgctctccct gccccgcctg ggcagtagcg tgccgccagg aaagcgtccc ttcggtagcg
    15301 cgactgccat gataatgaac cggatcgccc gcatagatga gatcctgcgt ccaggcggtc
    15361 atcgttcgcg cccccgtggt agctggcgaa gcagaaacac gctgcgtagc gctgctgtcc
    15421 gggatggtca ggcaatcgcc ttcccgatcg gcgttttgct gtaaaaacgt gcgaatcgcc
    15481 acggtcatgg ctttcgctgc ctcaggaggc gtacttttcg cctcgcgatc cagaactctg
    15541 gcaacgtact cttcacgatc gagatgcgac tgtaatttca gtttgccctt taccgtcagt
    15601 aacgttgtct caccgtggct gacaaatgtc atgtggttac cattggcaaa cgtcacacga
    15661 tagcgcccat tgagcacgcc tagtttgact gccgtcgtac tcttttcctc agtaactttc
    15721 ttcagcggat aacgagcaaa cagatccacc agcacacatt gcccactggc aacttgcgtc
    15781 ggcactggca aaacccgatt aagcgccgtc gcataacggg tcagtaccgt tttgctggtg
    15841 ccactgccag taacccacag cggtgtacca tcggttaacc agccagcaaa accaccctga
    15901 cggatttcct gtttatcatc ggcaaaccag ctccaggttt tcacccgtac ccgactaccg
    15961 agcatggatg cgacaccgat tttcgcctcg tccagcacca catccagtaa gacttcctga
    16021 gctttgtttt gcgcaggtaa cgtcgctaat gaatcaagca gcgatttaac cgttaccgag
    16081 gtttctggct tcagtgtagt aagagacgct aaccacgcag gtgcctgacg ctgttgccag
    16141 tattgccccc acatatctgc gccaatatgc aaccgctgcg gcgcaaaata gagtccacag
    16201 gaacgtacca gcgcggaatc gcgcgttatg ctctccccag cctgacaaca ataaacctct
    16261 tccggggagt tgccgcgaca ctggtatggc tgctccggtt gatgggtatc ctccagccag
    16321 gcgtaaacat acagtttcca caaactccct aatggcgtgg tgagcgtgtc aggtaatacc
    16381 gagactttgg tcacgcctga tgacgacagc tgatacagtt gatcgtgctg cgcaccacgc
    16441 agcgccagct gtaacggcgt ctcctccgca agcgttggca gagttaccaa cgccaatagc
    16501 cagacgattc tgtaccagtt cacttatttc acctgcatcc cgatccattc gctccctggc
    16561 gcaacacttt gctgtgcagg tgcataggaa cgcacataac gggcaggcgg cagaacgaat
    16621 tgcccttttt gcgagaaacg cagcaaatgg cggaaggtga ccgttcccgt cagttctttt
    16681 accggcacca tataggccag ttcacccatt tcattgcgcg ctttttccag cagttgtcct
    16741 tgctgttttc cggcgttcgg tttattgacc gaaatgcccc aggttgtacg ctcaacgtca
    16801 gcccccggcg gcagcggtac ttccacctga ccatagcgca gaacggcatc ctgctcgctg
    16861 gtaagcgtga tttcatcgag atacagcgca tcgctgtcaa tctcattgct ggtgacaggc
    16921 tgtaaggtaa agctcatctc ttcttcaccg gggataagcc gatacaactg gcgttcaacg
    16981 gtcaccggaa tattactttg ttgagccgtt tttgccggtt cacgccagcg gacctgcacg
    17041 ttttgcggcg gtaattcgtc accaaaagag agaatgtccg gcacgccctg accaacccaa
    17101 cgccagtatt cgcctcctcc ggttaactta tgtttggccc atgcgcccgc aggcgcaggt
    17161 aacacaaccg gaggcatcgt cgccatatat ttcgccagcc agttcatggc caacgcgcgc
    17221 tcaatggtgg attgctcagc ggttaaacca cttaaaatag cggccgcctg ggtagcgtcg
    17281 cctccactat tgagcagcac aaccgtgtgc gccagcggtt ggttgctgtt tgctgcttta
    17341 tcccacgcat attgctgaac ctgtggcatc acagtggact tcaactgcgc ttttttcagc
    17401 aacgtcatgg ttaagacgtt tgccaccgca tctgccagtg gagattcagg tgtatcgagg
    17461 atcatgctgt cattgatatc gctggtgtct tcctcagaga aatcttcagt tttagttccg
    17521 cgccgggcga tagcttcatc caaccctttc aacaacgttt tgcacggcag attcatctcc
    17581 tgtgcccacg ccagcaccag cgcccgatgc aataacggca tgttgtccgc ctgctcggca
    17641 taactgtcga gcatatgttg ccagtattcc ggttgctgcg ttacgccgag cgcctggctg
    17701 gcctgccagt cggcgtacca tgcccatgcc gtgaggaagg cgtcgccatt gccatcttca
    17761 ccccaccagg taaagcgcgc tccgggcccc gccagttgca tcagccgcag acggttatcc
    17821 tgaatcatct gacgaatgtc gttagcggcg gcactttgat gatcggcaag cgaacgccag
    17881 gcgagactga gcgggatcag acggctaccg gtgttgatca cgccacccca cggttcatcg
    17941 actaacgcat caagattgtt acggaaaatc tcctgcggcg tttcgctact ttgtagccgg
    18001 atattgctcg cctgctcggg caacatcagc gcattatcgc caccgccgag catgacattt
    18061 ttctgttgtt caacgggcca cctgttatcc acaaaagaca gttttgtgct aatgctgtcc
    18121 tgtacttgcc cattttgttg cagttctgca cttaacaagc cagattgctg aatattctgc
    18181 gtcagcgaaa tataattcgc ccctttgtgc agcgtcagcg tctggcgcat ctcagcacct
    18241 gcaaatttag tcaccagcgc caccggttcg ttatcctgct gactgaagat aaacagtcct
    18301 gccgccggtt tgtcacccat gcgatatacc gttggcatac tccacttcat gtagagattt
    18361 ttttccgaac gcaaataagc acgcccctgc ccgaccagcc cgtcgccgtt catcccacgc
    18421 gcggtgatgc gccagcgggt taacgaatca ggcatcagga acgtgaaata cgctttgcct
    18481 tgtttatcgg ttgtgagtga cggcatccag gccgcggtat ccacctcttc acgccgtgga
    18541 cgttccagca tttttactcg ccgctcgctg cggttagtcg cgccaggcgc aaccggctcg
    18601 ctggagagcg cctgatcgta gctgataaac gacagactgg agctggtacg cacattgtta
    18661 cgccccagcg gatagaaaaa tttgccgata ttcggcgcga tttccggttg cagcgcgtag
    18721 atcatttcat cgaccacgcc taccgttaac tgcgcagaaa caggtttacc tttcagcgac
    18781 gaggttaatt cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc
    18841 cggatatcca gctggggaac ggcaacttta atcccggcgt tctgaaaact gtactgaccg
    18901 ttacgggtat acagcaccga aaaagtgatg ttaggcgcaa aggaattgct caccggtacg
    18961 cgggcttcat actgggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgat
    19021 agcagcgact gctgttccac gcgatcgcgt tccagcgtca ataatgcttc gtcaattggc
    19081 tccgggaagg taatcagcat cttcgcggtt tcgccaggct gatacagtgt tttatctgcc
    19141 acgatatcta ccgtaccact atgcgacgtg ctgtccttac cgctgactgc atggcttaac
    19201 ccggcgagaa ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg
    19261 aaattgacgg taaaggattt gccgcctgac tgtagctctc cgctgtgact cgtgcggtct
    19321 tcgagacgca accattcata cgtaacagga acctgttttg aagattccag cgcggcataa
    19381 cggaacacaa ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc
    19441 gccaggccac gttcaatgag gatctctttg gtggtggtaa ctcgatacgc cgcgccgtca
    19501 ctggcggaga cggttaacaa ataacggctc ggtttatcgg cggcggggag attcaacgcc
    19561 acatgaccat tatcgtcgga aaccgtttca ctgccttcca tcgacaccgg gaaacgtccg
    19621 gcataacgca aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg
    19681 cgggcatctt ttaccggctc gccatccggg taaagcagtt gcagtttgcc gctgaccgct
    19741 tcgccagttt tgaactcttt tttgtcgaga gctaaaccaa tctcaaaatg gggtttgatg
    19801 tagtttgcca cgcgaaaact gctgctatag acctgtttgc ggtaagcaag acgtaactca
    19861 taacctccgg cgacggcatt ttctggcagg cggaaactcc cctgcccgcc attacgcgca
    19921 tccagcgtga cattgacggt ttgcaacaga ctgccgttgg cgtccagcac cgaaagcttc
    19981 gccggtgcgc tgacgatggg ggatgaatgc aacggatcgt ggaactcgcg acccatcact
    20041 ttaacatcga cacgatcgcc tgcgcgatat agcgggcgat cggtaaagat atacaagcgg
    20101 gtgttgtaga tttcgctttc gtagaaaaag ttctcggaga caaaaacgcc gccttcagca
    20161 tccttaccca gaatgtatga acgttctggc gatatatgtt gtaactgcaa ggtaccgctg
    20221 ccatcggtca caccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg
    20281 ggcttcgctt caccctgttt tttccccgcg gtccacacca gaagctcgtt acctgacact
    20341 ttgctaagcg ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacccacc
    20401 atcgcttcga cgagatacag tcccggctct tgtttgccga gaggaatata gatgttgccc
    20461 ggctgcggcg agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg
    20521 accggttttg cctgccatag tggatagcgg aactgttcca caagtggata ttttttcagc
    20581 ggtgaaaact ggctgttctg tacataacgg gaaggtttaa caatggcatt accgagctgt
    20641 aattcgggta acgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc
    20701 acgcggcgag atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagtccg
    20761 tcgcccagat attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat
    20821 gccatcgggt caggaatacg atacaggcga acgtccacgc cgccgtactc ttccatctga
    20881 tagcgccgat aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta
    20941 aaactgctgt cagcgagcaa aaagaatgtt cccccggcgg gcggcacata gttactggaa
    21001 ggaagcgaat cctcagcatt agcaagccct gttcccacaa gagacaggga caagcacgct
    21061 gctatcgcgc cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg
    21121 gtatccatcg ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt
    21181 tgtcagtttt cgtggcgctt ccggtgtggt agatgatgta acgccccatc cagaccatta
    21241 agtgctgggc atcgccctga tcgaaaaaaa tcatatcgcc aggcagtgcc tggtttatgt
    21301 cctggccaat aaactggctg ttgtactgaa tcaaattaat cgcggtcaca taggggccgg
    21361 ttttcccatt cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca
    21421 tctctggcgg caaatactgg ttagataaac cgttactttt tagccattta ctgtcgtgaa
    21481 ctttcagcgc ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc
    21541 gtgggcttgg cccctggcgg agctgttcct gtgcaatgcg cacaaaccac gcgcgaaaca
    21601 gcccggattg ttcgacattt agcatttcgc tatgggcaac aacacaatac agccagcaaa
    21661 tcagcgccag cagcccgtgt ctcatagcgg ttgccaggtt atgggtagcc actgccaggc
    21721 gacaccgggt tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc
    21781 cagcttcggc attaataaag tttgtacggc gttataaaaa accggttcga gattcttcgg
    21841 cagactggtc agcgtttcgt tacgcagcag tttcgccatt ccttgtggat tgatatagag
    21901 cggaacgatg ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt
    21961 ttgcagggcg ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat
    22021 cgccagcgac acgcggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc
    22081 tttcggatac tgaccgtatc gggaactcac ttcgcgacgc cagatttgcg cttcgccctg
    22141 ctgagtctgg cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt
    22201 ttgcgtaaac agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccaac
    22261 aaacagtggg gtttgcaatt ttgagtcttc ataccagcac agccccgcgc caccgtctaa
    22321 cgccccattc agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc
    22381 gtgtgaatac ggcaccgcca cacagaagct ggctccggca ggcatactgt tccataccgg
    22441 cgtaaaatcg aaactggcat ctacgctggc ggattcatcg tttaacgcca gaaaactgtg
    22501 ccagccgtcg ttacccattt cgaagcgtac gccagcaaaa gaaggcatta atcgctggta
    22561 gccaaacccc agcaacctgg cgctgactac gatgcgctgg cgtactggcg ttttttcggc
    22621 agtacgcgct tccaggccaa agcgggtttc ccagcgtttt ttaccactca acaaatcact
    22681 tgcgatcgcc gtggcttcgg tatcctgttg gtcatcttta aacagcatat ccgaactgga
    22741 aaaagccaat attttgtcct gataagtcgc aaacatcagg acgttattgc cgttatagcg
    22801 caactgataa acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttact
    22861 taactggctg tcgctggtag cggcaaacag caatggctcc agcagcttgc ttaaaccact
    22921 tcgctggatc agcaccatat aatgagaaag atggccttgt ttatcgtgcc acagtgccgc
    22981 ctgcgcgggc tggtctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc
    23041 atagacaatg cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata
    23101 gaaaacgaaa tcttcgctca gaacatcgtg gagaaacgga atggtaagga gatctttggg
    23161 aagctggctc agagagtcgc tgtcgagaaa gaggtccggc tcattgagat cgatttgcag
    23221 attgttgtgc accaccagtg gcgacaacgt tttttctggc ccactgccag agtattgtaa
    23281 tgcccatacg ccagcggata gcagcgctat tgccccaaaa ccaacaaggc cataaaaccg
    23341 ccagcctttc gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt
    23401 ttttacgggc acagccaaac tttaccgcgc cctaatacga caaaagccca gactttacag
    23461 cctggacttt tcaa
//
LOCUS       DAOFSV010000061        23281 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.061, whole genome shotgun sequence.
ACCESSION   DAOFSV010000061 DAOFSV010000000
VERSION     DAOFSV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.061"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1114)
                     /gene="rnd"
                     /locus_tag="QBH27_002535"
     CDS             complement(<1..1114)
                     /gene="rnd"
                     /locus_tag="QBH27_002535"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDQ7014835.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1196..2881)
                     /gene="fadD"
                     /locus_tag="QBH27_002536"
     CDS             complement(1196..2881)
                     /gene="fadD"
                     /locus_tag="QBH27_002536"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDQ7014836.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3086..3667)
                     /gene="yeaY"
                     /locus_tag="QBH27_002537"
     CDS             complement(3086..3667)
                     /gene="yeaY"
                     /locus_tag="QBH27_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDQ7014837.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(3707..4402)
                     /gene="tsaB"
                     /locus_tag="QBH27_002538"
     CDS             complement(3707..4402)
                     /gene="tsaB"
                     /locus_tag="QBH27_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDQ7014838.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLHDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4460..6370)
                     /locus_tag="QBH27_002539"
     CDS             complement(4460..6370)
                     /locus_tag="QBH27_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDQ7014839.1"
                     /translation="MTDDFAQDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6502..6846
                     /locus_tag="QBH27_002540"
     CDS             6502..6846
                     /locus_tag="QBH27_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDQ7014840.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            7208..7567
                     /locus_tag="QBH27_002541"
     CDS             7208..7567
                     /locus_tag="QBH27_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDQ7014841.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(7687..7866)
                     /locus_tag="QBH27_002542"
     CDS             complement(7687..7866)
                     /locus_tag="QBH27_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDQ7014842.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            7940..9301
                     /gene="pabB"
                     /locus_tag="QBH27_002543"
     CDS             7940..9301
                     /gene="pabB"
                     /locus_tag="QBH27_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDQ7014843.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSHLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLLEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            9305..9883
                     /locus_tag="QBH27_002544"
     CDS             9305..9883
                     /locus_tag="QBH27_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDQ7014844.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEGEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            10067..11431
                     /gene="sdaA"
                     /locus_tag="QBH27_002545"
     CDS             10067..11431
                     /gene="sdaA"
                     /locus_tag="QBH27_002545"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDQ7014845.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            11562..13160
                     /locus_tag="QBH27_002546"
     CDS             11562..13160
                     /locus_tag="QBH27_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ7014846.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPVMPLR"
     gene            complement(13164..14720)
                     /gene="yoaE"
                     /locus_tag="QBH27_002547"
     CDS             complement(13164..14720)
                     /gene="yoaE"
                     /locus_tag="QBH27_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDQ7014847.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            15184..16155
                     /gene="manX"
                     /locus_tag="QBH27_002548"
     CDS             15184..16155
                     /gene="manX"
                     /locus_tag="QBH27_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDQ7014848.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            16218..17018
                     /gene="manY"
                     /locus_tag="QBH27_002549"
     CDS             16218..17018
                     /gene="manY"
                     /locus_tag="QBH27_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDQ7014849.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            17031..17882
                     /gene="manZ"
                     /locus_tag="QBH27_002550"
     CDS             17031..17882
                     /gene="manZ"
                     /locus_tag="QBH27_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDQ7014850.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            17937..18395
                     /locus_tag="QBH27_002551"
     CDS             17937..18395
                     /locus_tag="QBH27_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDQ7014851.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            18824..19390
                     /gene="mntP"
                     /locus_tag="QBH27_002552"
     CDS             18824..19390
                     /gene="mntP"
                     /locus_tag="QBH27_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDQ7014852.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(19387..20196)
                     /gene="rlmA"
                     /locus_tag="QBH27_002553"
     CDS             complement(19387..20196)
                     /gene="rlmA"
                     /locus_tag="QBH27_002553"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDQ7014853.1"
                     /translation="MSFSCPLCHQPLLREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLREWLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(20362..20571)
                     /gene="cspE"
                     /locus_tag="QBH27_002554"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(20362..20571)
                     /gene="cspE"
                     /locus_tag="QBH27_002554"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDQ7014854.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(20584..20727)
                     /locus_tag="QBH27_002555"
     CDS             complement(20584..20727)
                     /locus_tag="QBH27_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDQ7014855.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(21397..21684)
                     /locus_tag="QBH27_002556"
     CDS             complement(21397..21684)
                     /locus_tag="QBH27_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDQ7014856.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(21759..21902)
                     /gene="mgrB"
                     /locus_tag="QBH27_002557"
     CDS             complement(21759..21902)
                     /gene="mgrB"
                     /locus_tag="QBH27_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDQ7014857.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            22061..22300
                     /locus_tag="QBH27_002558"
     CDS             22061..22300
                     /locus_tag="QBH27_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014858.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(22444..23235)
                     /gene="kdgR"
                     /locus_tag="QBH27_002559"
     CDS             complement(22444..23235)
                     /gene="kdgR"
                     /locus_tag="QBH27_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDQ7014859.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
BASE COUNT         5813 a         5959 c         5638 g         5871 t
ORIGIN      
        1 actgtggata ttcctgcaat aaattgtgta atgcttccgc catcagctca ccacgccagc
       61 cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt
      121 ggttgatttg ccgacgcgat gccagcaatt cggcgctgat cttgtgcgtt tcgctgacgt
      181 cagtaatcag cgacttaatc gctttaaacg ctttacgata acccggcatg tccatcaggt
      241 taagcatcgg ctgcggtaag gcctcttccg gcaatgcctg cgctttttcc accagcgcca
      301 gcaacgtttt accgtggaag cggatttcgc taccggataa gcccaggctg tccagttcgc
      361 ccagacttcc cggcatataa cgcgctaccg accacaaatg ctcttcacgc acgacaaagt
      421 tcaccgccag atcgcgctct cgcgccttgc gcaatcgcca gtcggctaac agttgcagac
      481 aggccagttg gcgtgtgcgt aactgccagg cattggtgat atcacgccag gcgtcttccg
      541 gcgcaacgac ttcctgacga cgcatttgca tcaggcggca ttcatccagc gccgcaggta
      601 gccagccgga ggcctccgtt tctaccataa gctttgcggt gatcggtaac agataccaga
      661 catccgccgc tgcgtattca cactgacgtt cggtcagcgg tctggccagc cagtcggtgc
      721 gcgattcact cttgtccagc gtaacgccgg aatactcttc caccatggaa gcgaaacccc
      781 atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta
      841 attcgccaaa gacattgagg aacacttcca gatcttcact gcctgcatgg agaaattttg
      901 tgatggacgg atcgcgcagg atcgctttca gcggtgacca gtcggtgatc ccgagtggat
      961 cgattagcgc cagatgctca ccatcaaaaa gttgaattaa acccagctgc gggtaataag
     1021 tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg aaaggcacgg acggcttcac
     1081 acaaagaagc cagcgcatcg tccgtggtaa tcatttggta attcaaattg tattctcttt
     1141 agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga cgactgactt aacgctcagg
     1201 ctttattgtc cactttgccg cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga
     1261 cgttagattt cggtaactca tcacgaaact ccaccagctt cggtactttg tagcccgtga
     1321 gctgacggcg gcaaaaggtc accagtgact cttcggtaag cgatggatct tttttcacta
     1381 cgaagatttt caccgcttca ccactggagc cggaaggtac gccaacagcc gcgacttcct
     1441 gtacgccagg atgctgcatg acgacatctt caatctcgtt gggatagacg ttaaaaccgg
     1501 aaaccagaat catatctttt ttacgatcga caatgcgcag gaatccttct tcatccatca
     1561 ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat ttcatcggta gcatccggac
     1621 gctgccagta acccagcatc acctgcggtc ctttgacaca aagctcacct ggttgaccag
     1681 gtggtacttc attatcatca tcatccacca gtttggcttc cgtcgacggc accggcaaac
     1741 cgatgctacc actatgataa tcaatatcat acgggttaac gctgaccagc ggcgcgcact
     1801 cggtcaggcc ataaccttcc agcagatact gtccagtcag tttaacccaa cgttctgcaa
     1861 ccacttgctg aacaggcatc ccaccgcctg cggaaagatg cagactggag aaatccagct
     1921 gctggaactc tttattgttc agcaacgcat tgaacaaggt gttaacgcca gtgatagcgg
     1981 taaacggata tttcgctaac tcttttacca gccctggaat atcgcgcggg ttagtgataa
     2041 gcaggttctg ccctcccagt tcgataaaca gcaggcagtt aatggtcagt gcaaaaatgt
     2101 gatacagcgg cagcgccgtc accaccagct ctttgcccgg atgcaacagc ggaccatagg
     2161 tcgcgttaac ctgttccagg ttcgccagca tattgcggtg agtcagcatc gcgcctttcg
     2221 ccacaccagt ggtgccgccg gtgtattgca ggaaagctaa atcttccggc accaattctg
     2281 gtttgacgta ctgcatccgg tagccgttgt gcagggcgct acgaaatgaa atggcatctg
     2341 gcagatggta tttcggcacc agacgcttga tgtatttaac aacgaaattg actaccgtgc
     2401 cttttgccgt agatagctga tcgcccatac gggtcagaat aacgtgctga acggcggttt
     2461 tatcaaccac tttttccaga gtgtgggcaa agttagacac gataacaatc gccgatgcgc
     2521 cgctatcgtt aagctgatgc tcaagctcac gcggggtata caacgggtta acgtttacga
     2581 cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg atattgcaat aagttaggca
     2641 tcatcaacgc aacgcgatcg cctttcttca gccccaaccc ttgttgcaaa taagcggcaa
     2701 acgcgcgact gcgttcttcc agtttgcgga aggtcatcac ctcccccata ttcacaaacg
     2761 cgggttgatc ggcatagcgt gcgaccgact gctcaaacat atctaccaga gattgataac
     2821 ggtcagggtt gatctccgtc ggaacgtccg cgggataacg gttaagccaa accttcttca
     2881 attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac cccaagtgat aaacatgcta
     2941 ttaacataat attaactcat cttaccagct tgataattac ccaaggaaaa ggttgcgaag
     3001 cgcgtcacta tttattttta tctttaccgt aagaatgcag aaacagcgga ccggccgctg
     3061 tttctttttc tttaaaaaca agcaattatt cagttacaac tgtttgtact ctcgcggggc
     3121 cgggattata ccagccccat ccgccgtagc catagggcca gccacgaccg ccataaaacc
     3181 atggttcaat cggctgaggc ggcataatca cctgctgggt taaatgccaa cgtttgtaac
     3241 ccgttacttg catcaccata aatttatagg gcgtattacc gattttgccg tcaaccgcac
     3301 cggtgattgg cccgactacc gtcaccagtt gtccgcggaa atccaccggg tccagaaaac
     3361 cgttcacatc ggcataaatg cgaccgcgag aaggttctcc cagcgtcggt ctggctccgc
     3421 tgtccagtgg tacggtggca atttccaggc gggttttccc ttgctggttt tgtacagcaa
     3481 ccactttgcc gccaaagcga gcctcctgac caacgtacag ctgcggcgca ctcatcaccc
     3541 gaactaaatc ttgttgcggc gtgggactac tgcctttaat ggcgtccggc acagtaacac
     3601 aaccgctcag cattagcgca aacgtacctg ccagtatgcc tttgataaca ttcttttgaa
     3661 ccgccatgat gcgactcctt tttttctcag ggcatactct taagattcat tctttgcccg
     3721 gaagtttctt ccatgcgaca ttgttacgta aataaactgg ttcggcatgt tccaccgcca
     3781 ccgttttacc ctcggcaaac atctggcacg caatcggcag catatcttct gcagcgggca
     3841 gtaacacttc gccatcgtgc aaaaccagcc cgctctcttt accgagatcc ggccaggctt
     3901 gccagcccgt ccctaccgtc acccattcgc cggaaagctg ttgcattcgt tcatgaacca
     3961 gttcgggttt gagtacggct tcggtttctt caccgtgcca gataccgttt tcatcacgct
     4021 gatattcggc ccagtaaact tcgcccattc gcgcgtcaat agctgccagc acgcgggttg
     4081 cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg
     4141 gcagctctgc gccaagtgcc agcccttgcg caataccaat accaatgcgc acgccggtaa
     4201 agctaccggg gccgcgcccg taagccagag cgtttatgtc agtcagggaa gttccgctgg
     4261 tggtcaggat atcctgcacc atcggtaaga ttcgttgagt atgttcacga gggcaaagct
     4321 caaaatgagc gttgacagta ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg
     4381 tatcgatagc cagaattcgc atgggtcttc gtgcttagat caataaaaag gcgcgcatca
     4441 taccatactc cgtaacaaat tacctggagg atggtatcgc aaggaaacga accgcacggg
     4501 caatgtcacg ggtgcgcggc gctggcggca gactggcgag aaacgtcgcg ccgtaagggc
     4561 gcatcaccag ccgattgtcg caaatcacca gcacgccgcg atcgtcggcg tcgcgaatca
     4621 atcgccctac cccctgcttg agagtaatga ccgcatccgg cagttgtact tcatcgaacg
     4681 gatcaccacc gcgcaaacgg caatcttcca tgcgtgcttt taatagtgga tcatccggcg
     4741 aggtaaacgg caatttgtcg ataatcacca atgacaatgt atcgccacgc acgtccaccc
     4801 cttcccagaa actgctggtt gccacaagaa gcgcgttacc ggcgctgaca aattgctgca
     4861 acagttgccc tttgctggtt tccccctgca acaaaacggg aagcgtcatg gtagcgcgga
     4921 actgctcggc cagatcgcgc atcatggcgt gcgaggtaca aagcataaaa caacgaccgt
     4981 tgttagcttc gatgatcggt cgcagcattg ccgccagttg ccgtgcggaa cccggttgat
     5041 tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg gcggctgtaa tcaaaggggc
     5101 tgggtaacag caatgactct gcctgttcaa tgccaagccg cgaggtgaaa tgatgcagat
     5161 cgtcgttcac cgacagcgtt gctgaggtaa agatccagct accggttttt tgcgccatta
     5221 actctttgaa tttatccgcc acgctgagcg gcgtgagagc cagagtaaaa tggcgcgaag
     5281 tgcattcgta ccagtagctg tagcccggct gattgatctc ttttagccgt ttcagccgtg
     5341 tgcgatataa cgtggcgcgc tcaaatgccg catccagcaa ggcggaacgc cccagcgaca
     5401 gtttcgccac gtcataacaa agttccagag tgtcatcgag cagtaaaaat gcccgctgaa
     5461 tttgcggatt agctaacagc tcacgcaggt taccgcgata accaggctca ccgagttgca
     5521 ggcgaaaatc ctgcgcactc tgggcaagac gatccgcgca cttttgtaac tgctgggtgt
     5581 cttttaattc ggtgcggtag gcgatggtga tgtcttttgc caggtcgagc agttgtcgac
     5641 tggagagtga ctgaccaaaa tactggctgg caatgtcggg cagctggtgg gcttcgtcga
     5701 agatcatgac gtccgcctcc gggatcagtt cgccaaaacc gctctctttc accaccatat
     5761 ccgccagaaa gagatgatgg tttaccacca ccacatcggc gtccatcgct tttttgcgtg
     5821 ctttgaccac aaagcaatct ttatacatcg ggcagtcgct gccgaggcag ttatcgttgg
     5881 tgctggtgac cagcggccac gcctgtgaat cttccgccac gctgacgcag gtgctgatat
     5941 caccatcgat tgtttgatta gaccaggagc gcagcaggat cacatcgctt aagatttgta
     6001 ccggcagatc gccccccgcc aaagcctgct gttcgagacg ttcgaggcag aggtagtttg
     6061 agcgcccttt cagcagcgcc acattgcccg tgtatttcaa tgcctttgag accgttggca
     6121 aatcgcggct gtagagctga tcctgcaacg cttttgagcc ggtcgagatt atgactttct
     6181 ttttcgcccg cagcgcaggg gccaggtaag cgtaggtttt gcccgtaccg gttccggctt
     6241 ccaccaccag cggctggcct ttttctatcg cctgggtgac ggctaccgcc atctgtcgct
     6301 gtggttctcg cggcttaaag cctggtatcg ctttcgccag ctgaccgtct tgtgcaaaat
     6361 cgtccgtcac actaccccct gttgatttga acagggatta tgtcaggatg agggcgcttt
     6421 cgccagttga agtggtgacg gcgacctcac attgtggcag tctttgcagc acgaaaatgg
     6481 aagtataatg aggacaaaat gatgactatc gttcgtatcg atgccgaagc ccgctggtct
     6541 gatgtggtaa tccacaacaa cacgctctac tacactggtg taccggaaaa cctcgacgcc
     6601 gatgcctttg agcaaaccgc caacacgctg gcgcagattg acgccgtgct ggaaaaacag
     6661 ggcagcaata aatcgagcat tctggatgcc accattttcc tggccgataa aaacgacttc
     6721 gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg gtcatgcgcc ggtgcgctgc
     6781 acggtacaag caggtttgat gaacccgaag tataaagttg aaattaagat tgtggctgcg
     6841 gtgtaagctt tatcgaagca aaataagtca gacgataatt tatcgacaat actggtcggt
     6901 tttgcaaaaa tcgaccagag attaagatat tacagttgat gctcctgcta attcattatt
     6961 tatttttata atttcaattt tatctataaa actaactata taaataaaca taatcatgcc
     7021 gttatgttcg cccttcgatt agcatctctg gttttctttc ttacctgaac aatctggtta
     7081 tagcgtgagg actgaccatt aattttctcc atcttactgg ataacacacc atgagctgct
     7141 acctttaaaa cacctgaatg tcaataggtt aactcgtgcc atatcgttat aatccctttg
     7201 atattagata caaattaagg tcatatatag ccttatcgat aacatggtta attttaagga
     7261 taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca tggatgtatc
     7321 agcgccaaca ccaagttcga tgaatgaaag tacggcgaag ggaatcttta aatatttaaa
     7381 agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc aggaaggctg
     7441 gaaccctggt ttcacggaaa aaatggttgg atgggcaaaa aaaatggagt ctggtgaacg
     7501 ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac taaaagcact
     7561 ggtttgagtt aaattcacat cagcataaat gggtcaggac gcttttaatc acatataaaa
     7621 agcgtccttt ttctcacaat caaataatca tcggcttcta taacgaaagc gatacggatt
     7681 agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg aatggttggc
     7741 acgcagctct tctgccacca gcgcgattgc ctggccgctg ctcatccctt gtgccatcag
     7801 ttcctggatc cgctcgacgg ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc
     7861 aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac ccggcacata
     7921 gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt taccctggcg
     7981 tcaggacgcc gctgaatttt atttctccca cttaagccac ctgccgtggg cgatgctttt
     8041 acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg ccgagccaat
     8101 ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaagcgaaa aacgcacaac
     8161 gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgtgcag acattcgccc
     8221 agcgcataac gaagatttgc catttcaggg cggcgcgctg gggttgtttg gctacgatct
     8281 gggccgccgt tttgagtcac tgccagaaat tgcggaacaa gatatcgttc tgccggatat
     8341 ggcagtgggt atctacgact gggcgctggt tgtcgaccac cagcgtcaaa cagtttcttt
     8401 gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc aattctcccc
     8461 gcaggaagat ttcacactca cttccgactg gcaatccaat atgacccgcg aacagtacgg
     8521 cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc aggtgaatct
     8581 cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc ttcagcttaa
     8641 tcaggccaac cgcgcgccgt ttagcgcttt tttacgtctt gaacaggctg ctattttaag
     8701 cctttcacca gagcggttta ttctttgtga taatggtgaa atccagaccc gcccgattaa
     8761 aggcacgcta ccacgcctgc ccgatcttct ggaagatagc aaacaagcag aaaaactggc
     8821 gaactcaacg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc gtaatgatat
     8881 cggtcgtgtt gccgtagcag gttcggtaaa agtaccagag ctgttcgtgg tggaaccctt
     8941 ccctgccgtg catcatctgg tcagcaccat aacggcgcaa ctaccagaac agttacacgc
     9001 cagcgatctg ctgcgcgcgg cttttcctgg tggctcaata accggggctc cgaaagtacg
     9061 ggctatggaa attatcgacg aactggaacc gcagcgacgc aatgcctggt gcggcagcat
     9121 tggctatttg agcttttgcg gcaacatgga caccagcatt actatccgca cgctgactac
     9181 cattaacgga caaatttact gctctgcggg cggtggaatt gttgccgata gccaggaaga
     9241 agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac tggagaagta
     9301 agacgtggaa taccgtagcc tgacgcttga tgatttttta tcgcgctttc aacttttgcg
     9361 cccgcaaatt aaccgggaaa ccttaaatca tcgtcaggct gccgtgttaa tccccatcgt
     9421 ccgccgaccg caaccggggt tgttgctgac tcagcgttcg attcatctgc gtaaacacgc
     9481 tggacaagtg gcattccctg gaggcgcagt cgatgacacg gacgcatcag ttatcgccgc
     9541 tgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg aggttatcgg
     9601 cgtgctgccg cccgtcgata gcgtcactgg ctaccaggta accccagtgg tcggcattat
     9661 cccgcccgat ctgccgtatc gcgccagtga aggtgaagtc tccgccgtct ttgaaatgcc
     9721 gctcgcccag gcattacatc tgggtcgtta tcatccttta gatatctacc gccgtggcga
     9781 ttcacatcgg gtatggctgt cctggtacga acagtatttt gtatggggaa tgaccgcagg
     9841 cataattcgt gagctggcgc tgcaaattgg tgtgaaaccc tgactatact tatctttaca
     9901 tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg tttttgatta
     9961 gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact atgcgctgtt
    10021 attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga ttagtctatt
    10081 cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc ctatgaaggc
    10141 gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg ttactcgcgt
    10201 tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca ccgatatcgc
    10261 cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca gtattcccgg
    10321 ttttattcgc gacgtagaag agcgcgaacg tctgctgctg gcacagggac ggcatgaagt
    10381 ggatttcccg cgcgacaacg ggatgcgttt tcataacggt aacctgccgc tgcatgaaaa
    10441 cggtatgcaa atccacgcct ataacggcga tgaagtcgtc tacagcaaaa cttattattc
    10501 catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg ccaacgaagt
    10561 gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta atgaaaccgg
    10621 cttttcgctg tctggtctcg ctatgcaaaa cgaactggcg ctgcacagca agaaagagat
    10681 cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc gcgggatgaa
    10741 taccgaaggc gtactgccag gcccgttgcg cgtgccacgt cgtgcgtcgg ccctgcgccg
    10801 gatgctggtt tccagcgata aactgtctaa cgatccgatg aatgtcattg actgggtaaa
    10861 catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggccgtgtgg taactgcgcc
    10921 aactaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc actttattga
    10981 atcagttagc ccagacatct atacccgtta ctttatggca gcgggcgcga ttggtgcact
    11041 gtataaaatg aacgcgtcta tttccggtgc ggaagtcggt tgccagggcg aagtaggtgt
    11101 tgcctgttca atggctgctg cgggccttgc cgagctgttg ggcggtagcc cggaacaggt
    11161 ttgtgtggca gcggaaattg gcatggaaca caacctcggt ctgacctgcg accccgttgc
    11221 agggcaagtt caggtgccgt gcattgaacg taatgccatt gcctctgtga aggcgattaa
    11281 cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc gtctcgctgg ataaggtcat
    11341 cgaaacgatg tacgaaaccg gtaaggacat gaacgctaaa taccgcgaaa cctcacgcgg
    11401 tggtctggca atcaaagtcc agtgtgacta atacttctta ttcgcccatc tgcaacggat
    11461 gggcgaattt attcccgctt tctcgtctgc tgtaattttc cccactacac ttccactgtt
    11521 gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa gcacaacgga
    11581 tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca ctcgttacgc
    11641 tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa caacgggtag
    11701 tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc ctacaggcag
    11761 gtagcgaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat ttgccattac
    11821 gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa gacggcacac
    11881 tttattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt ctggctgaac
    11941 ttcctgcacc gcaaccactt ttacgactga cgaccgaccg tgccctgata aaaggcagtc
    12001 cggttttgat tcaatggacg cctgcagcgg gcagtagcaa tgctggggtc atggagatga
    12061 ttaacatcga cttactgacg gcaatgctcc ttgaaccaca actgccgcaa atcagtagcg
    12121 ccagcctgac ggtggacagt cggcatttgc tctatggtaa tgggctggta gattcccttc
    12181 cgcaacctga aaacaatgaa aactatcagg tttcttcgca acgctttcct tttaccatta
    12241 acgttaatgg cccgggggct acggcgctgg catggcacta tcttccaaca caattaccgc
    12301 tggcggtgct gctaagttta ctggtgggct acatcgcctg gctggcgacc gcttaccgaa
    12361 tgagcttttc ccgcgagatc aatctgggcc tggcgcaaca tgagttcgaa ttgttctgtc
    12421 agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg ctgcgctgga
    12481 acaatccgcg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg gaagaacatc
    12541 atttaattgt gccactgacc cgctatgtga tggcagaaac cattcgtcag cgccatgttt
    12601 tcccaatgag tagtcagttt catgttggca ttaacgtcgc acccagccat tttcgccgtg
    12661 gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt cagcaactga
    12721 tcctcgaaat caccgaacgc gatgccttac tggatgttga ttaccggatt gcccgcgagc
    12781 tgcatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac agttcatttt
    12841 cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt accgcagcta
    12901 taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcactg gggcaaagac
    12961 tgaatattga actggtggcg gagggcgtag aaacgcaaga acaggcgaag tatttgcgca
    13021 gtcatggcgt gcatattttg caagggtatt tgtacgcaca gccgatgccg ctacgtgatt
    13081 ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga catatcacgc
    13141 ccgttatgcc gttacgttaa cgcttactca tcttcatcgt gcgccggttg ctctttaaca
    13201 atgcgaacca gatcaacacg ataatcattg gcttcaatga tggtgatatg cagtggacct
    13261 acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac gaggcccgcg
    13321 accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg ctgcaaggca
    13381 tgcaaatctg taccgccttt taccagccag ccgtcaccgt cggtaatgat ttccggcgtt
    13441 tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg cgtgaccaga
    13501 ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc acgacgcagc
    13561 acgcccagca ggttgatcgg atcgagggtt tccgggacga taatcgccgg agacgccgaa
    13621 gctatcgccg ccacatcaac tccctcttcc agcgccacca gcagttcttt agcacgtacg
    13681 atgccgataa tttcatccag ttcaccgcga cataccggga acaaactgtg cggtgaggag
    13741 agcagttgct cgcggatttc atcgaccccg agattagcat caacccagct tatttcaccg
    13801 cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tcagtacgcc gttaatcatg
    13861 taacgttctt cttcggcaaa cgcaccttcc gggatcggca tcggcatcgg gttatcggca
    13921 tcgtgctgaa cattggcctg acgtttcccg cccatcaaac gcaggatggc atcggcagta
    13981 cgcgctcgca gcggcaaagt cgactggtga cgaataaagt tgcgacgcgc aatctggtta
    14041 aacacttcga tgatgatcga gaagccaatc gcggcataca ggtaaccttt cggaatgtgg
    14101 aaaccgaaac cttctgccac cagactcaga ccaatcatta acaggaagct cagacagagc
    14161 accaccaccg tggggtgctg gttaacaaat cgcgtcagcg gtttcgatgc cagcagcata
    14221 accgccatcg caatcactac cgccgccatc atcaccggca aatggttaac catccctact
    14281 gcagtaatta ccgcatccaa cgaaaagacg gcgtcaagga tgacaatctg tgtgacgacc
    14341 acccagaaac tggcgtagcc tttaccgtgg ccggaatcat gatcgcggtt ttccagccgt
    14401 tcatgcagtt cggttgttgc tttgaacagc aagaatatgc ccccgaacaa cataatcagg
    14461 tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg gtttggtcag cgtgaccatc
    14521 catgaaatca gcgacagcag ccccagacgc ataatcagcg ccagtgataa tcccagcaaa
    14581 cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa gaatggcgat gaagaccagg
    14641 ttatcgatac ccagcacaat ttcgagaaca acaagcgtga gtagccccgc ccaaatcgag
    14701 gggtccatta agaattccat gacaagctcc tgcttaagga atagctattc gacgccagaa
    14761 ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg gcataaagtg ccagaaagac
    14821 aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg atccatactg cgggctactg
    14881 ccctatactc catggttgtt aaacaggagt taaacatatc agagacgcct ctgatttggc
    14941 aaagatttac cttcctttgc aaacgaatgt gacaaggata ttttaccttt cgaaatttct
    15001 gctaatcgaa agttaaatta cggatcttca tcacataaaa taattttttc gatatctaaa
    15061 ataaatcgcg aaacgcaggg ggtttttggt tgtagccctt atctgaatcg attcgattgt
    15121 ggacgacgat tcaaaaatac atctggcacg ttgaggtgtt aacgataata aaggaggtag
    15181 caagtgacca ttgctattgt tataggcaca catggttggg ctgcagagca gttgcttaaa
    15241 acggcagaaa tgctgttagg cgagcaggaa aacgtcggct ggatcgattt cgttccaggt
    15301 gaaaatgccg aaacgctgat tgaaaagtac aacgctcagt tggcaaaact cgacaccact
    15361 aaaggcgtgc tgtttctcgt tgatacatgg ggaggcagcc cattcaatgc tgccagccgc
    15421 attgtcgtcg acaaagagca ttatgaagtc attgcaggcg ttaacattcc aatgctcgtg
    15481 gaaacgttaa tggcccgaga tgatgaccct agctttgatg aactggtggc actggcagta
    15541 gaaacaggcc gtgaaggcgt gaaagcactg aaagccaaac cgattgaaaa agccgcgcca
    15601 gcacccgctg ccgcagcacc caaagtggct ccaactccgg caaaaccaat gggaccgaac
    15661 gactacatgg ttattggcct tgcgcgtatc gacgaccgtc tgattcacgg tcaggtcgcc
    15721 acccgctgga ccaaagaaac caatgtctcc cgtattattg ttgttagtga tgaagtggct
    15781 gcggataccg ttcgtaagac actgctcacc caggttgcac ctccgggcgt aacagcacac
    15841 gtagttgatg ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc tggcgaacgc
    15901 gtaatgctgt tatttaccaa cccaacagat gtagagcgtc tcgttgaagg cggcgtgaaa
    15961 atcacctctg ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca ggtgaataac
    16021 gcggtttcgg ttgatgaaaa agatatcgag gcgttcaaga aactgaatgc gcgcggtatt
    16081 gagctggaag tccgtaaggt ttccaccgat ccgaaactga aaatgatgga tctgatcagc
    16141 aaaatcgata agtaacgtat tgtgttgatt atcactcagt tttcacactt aagtcttacg
    16201 taaacaggag aagtacaatg gagattacca ctcttcaaat tgtgctggta tttatcgtag
    16261 cctgtatcgc aggtatggga tcaatcctcg atgaatttca gtttcaccgt ccgctaatcg
    16321 cgtgtaccct ggtgggtatc gttcttggcg atatgaaaac cggtattatt atcggtggta
    16381 cgctggaaat gatcgcgctg ggctggatga acatcggtgc tgccgttgcg cctgacgccg
    16441 ctctggcttc catcatttct actattctgg ttatcgcagg tcatcagagc attggtgcag
    16501 gtatcgcact ggcaatccct ctggccgctg cgggccaggt actgaccatc atcgttcgta
    16561 ctatcaccgt tgctttccag cacgctgcgg ataaggctgc tgataacggc aacctgacag
    16621 cgatttcctg gatccacgtt tcttctctgt tcctgcaagc aatgcgtgtg gctattccgg
    16681 ccgtcatcgt tgcgctgtct gttggtacca gcgaagtcca gaacatgctg aatgcgattc
    16741 cggaagtggt gaccaatggt ctgaatatcg ccggtggcat gatcgtggtg gttggttatg
    16801 cgatggttat caacatgatg cgtgctggct acctgatgcc gttcttctac ctcggcttcg
    16861 taaccgcagc attcaccaac tttaacctgg ttgctctggg tgtgattggt actgttatgg
    16921 cagtgctcta catccaactt agcccgaaat acaaccgcgt agccggtgcg cctgctcagg
    16981 ctgctggtaa caacgatctc gataacgaac tggactaaca ggtgagcgaa atggttgata
    17041 caactcaaac taccaccgag aaaaaactca ctcaaagtga tattcgtggc gtcttcctgc
    17101 gttctaacct ctttcagggt tcatggaact tcgaacgtat gcaggcactg ggtttctgct
    17161 tctctatggt accggcaatt cgtcgcctct accctgagaa caacgaagct cgtaaacaag
    17221 ctattcgccg tcacctggag ttctttaaca cccagccatt cgtggctgcg ccgattctcg
    17281 gcgtaaccct ggcactggaa gaacagcgtg ctaatggcgc agagatcgat gacggtgcta
    17341 tcaacggtat caaagtcggt ttgatggggc cgctggctgg tgtaggcgac ccgatcttct
    17401 ggggaacggt acgtccggta tttgcagcgc tgggggctgg tatcgcgatg agcggcagcc
    17461 tgttaggtcc gctgctgttc ttcatcctgt ttaacctggt gcgtctggca acccgttact
    17521 acggcgtagc gtatggttac tccaaaggta tcgatatcgt taaagatatg ggtggtggct
    17581 tcctgcaaaa actgacggaa ggggcgtcta tcctcggcct gtttgtcatg ggggcattgg
    17641 ttaataagtg gacacatgtc aacatcccgc tggtggtctc tcgcattact gaccagacgg
    17701 gcaaagaaca cgttaccact gtccagacta ttctggacca gttaatgcca ggcctggtac
    17761 cactgctgct gacctttgct tgtatgtggc tgttgcgcaa aaaagttaac ccgctgtgga
    17821 tcatcgttgg cttcttcgtc atcggtatcg ctggttacgc ttgcggcctg ctgggactgt
    17881 aagactgttg tacactaccg gggccttttg gccccgtttt tttatctgga ggattaatga
    17941 caatcacgga cctggtactg attcttttca tcgccgcact tctggccttc gcgatctacg
    18001 atcagttcat catgccccgc cgtaacggcc ccaccctgct ggcaattcct ttgctccggc
    18061 gtggtcgcat cgatagcgtt atcttcgtcg gattgattgt cattcttatc tataacaacg
    18121 tcacgaatca tggcgcgctt ataacaacat ggttattaag tgcactggct ctgatgggtt
    18181 tttatatatt ctggattcgc gttccgaaga tcatctttaa acaaaaaggt tttttcttcg
    18241 ccaatgtctg gattgaatat agccgaatca aagcgatgaa cttgtcggaa gatggcgtgt
    18301 tggtgatgca attagaacag cgtcggctgt taatccgcgt tcgaaatatc gacgatttgg
    18361 aaaaaattta taagcttctc gtttcaactc aataagttat gaatttagcc aaagctatgt
    18421 ttagtgtatt tttaataatc atacatagct taggctatat tagctcttcc cttatttgtt
    18481 atttatttta acgtttcatt gatatataaa tctaaatgaa aatcgttatc aataaaacaa
    18541 taaaataata tattccaaca gttgtcttat attctcaaaa tatgttaagg ttgcgccctc
    18601 atttggggag tagctgattt ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa
    18661 aacgtggcac gtacggactg aacactttca gtcaggcgag accatatgca catcaatcgc
    18721 tatgcctgca tgaacgcgaa aaccatcgcg gcttgttgtt catgtatgca tgtttgctgg
    18781 gggcgatgat gtgttttatg gataccccgg tcaggacgtt gtcatgaata tcactgctac
    18841 tgttcttctt gcgtttggta tgtcgatgga tgcctttgct gcatcaatcg gtaaaggtgc
    18901 caccctccat aaaccgaaat tttctgaagc attgcgaacc ggccttattt ttggtgccgt
    18961 cgaaaccctg acgccgctga tcggctgggg aatgggcatg ttagccagtc ggtttgtcct
    19021 tgaatggaac cactggattg cgtttgtgct gctgatattt ctcggcgggc gaatgattat
    19081 tgagggtttt cgtggcgcag atgatgaaga tgaagaaccg cgccgtcgac acggtttctg
    19141 gctactggta accaccgcga ttgccaccag cctggatgcc atggctgtgg gtgttggtct
    19201 tgctttcctg caggttaaca ttatcgcgac cgcattggcc attggttgtg caaccttgat
    19261 tatgtcgaca ttagggatga tggttggtcg ctttatcggc tcaattattg ggaaaaaagc
    19321 ggaaattctc ggtgggctgg tactgatcgg tattggcgtc cagatcctct ggacgcactt
    19381 ccacggttaa taagaacgct gccagaggtg aatattaaag tccgtctggc agtcgaacac
    19441 ttcttttgct gccagtgctt gccagacttc aggcttcgca cgccaggcaa acggcgtcat
    19501 ctgcaataat gcgacggctt catcaccgcg aagacgcatc agataacaca actcatcact
    19561 ctgctgtaat gtaaaacctt tcagttgttc tgcatgaggt gcatgaagat gtacttcatt
    19621 gtaaatcagc cccttcagct ccattaagtg tctcggtccc ggcgtggcag taatgaccca
    19681 accgccgggc ttcactactc gcactaattc ttctgcttta cacggcgcgt aaatacgtat
    19741 tatggcgtcc atactggtat cggaaaacgg caaacggtgg ctggaagcga cacaaaaagt
    19801 gacctgcgga tagcgtttcg ccgccgcttt tatcgctacc ttcgagacat ccaggccaaa
    19861 cgtggtgatt tcgggcaacg catcagcaaa tgcgtgtgtg taataccctt caccacagcc
    19921 aatatccagc accgccgtgg ccttttcatc aagccattcc cgcagttggg cgacaattgc
    19981 atcacgcagc ggctgataat gtccggcatc taagaatgcg cggcgtgctt gcatcatttc
    20041 cgcgctgtcg cccggatcac gagaccgttt atgctgaacg ggcagcagat tgacataccc
    20101 ttctttcgcc atatcaaact gatgtcgctg gggacagata tagctgtttt tttcacgcaa
    20161 aagaggctga tggcaaagtg gacaggaaaa cgacatgaca actccggcag gatactaaag
    20221 gccgcaagtg taacgcgaat catgcggcgg gcaaatatcc gaacgactgg agcgaagata
    20281 aaacaaagta aaaagcctcg cattcgacga ggctttatag cgagattgaa gcgtattcac
    20341 acttcagatc agtggattcg atcagatagc tgttacgtta acagctgccg gacctttctg
    20401 accgtcctga atttcgaact caacgttctg accttcagcc agagttttga aaccattacc
    20461 ctggatagcg gagaagtgta cgaacacatc tttgctgcca tcagccggag taatgaagcc
    20521 aaaaccttta gactcgttga accacttaac ctgaccttta atctttgcca tttgaaaaat
    20581 tccttagatt gttttcttcg cccgcaggca taacatagat aaaactgaca cattactgca
    20641 tgaggcacca atataaggct cggcagagaa gcggtattca acgtcaacgt gtttactcag
    20701 gacttcttta ctgaaaatgc cacacataaa cagaactgta cctcgtttaa cccgaaatct
    20761 gttatcacat acaacgttaa ttatggcaag ccatttttaa acatgtctcg atcagacgca
    20821 caaatctcga tacgtcttcc actttttttg cacacttatg caacggaata cgcgccgata
    20881 tatcattgtg cttaaccttg ccagttcagg cagatactta aactgacgta ttttctaaca
    20941 tagttcaata agttcagttg ttccaggcca gtgggagaag ttattacata gtgcgtgcaa
    21001 tatcacattt tctactatgc aatgaataaa aagttatatc actttttctc ataaaacagt
    21061 cagttaacgg gtatcaatta ccccaaagag aagtcaatct ccaaaggtat tgtaaattta
    21121 aaataagaaa aattgatgaa ttagcaaaaa aatcaagaga gaaacgtttc gctaataaat
    21181 aataccacac ttaatcacaa atccgatgta aacatcctaa taaattaatg gggtaaatat
    21241 ctttttctga tatcccgtta cgcaaataaa taatatgata attatttgaa gtcagaccag
    21301 agttttaatt tgaagcagtt gcaccatgac agtgcgtata atcagcatga attgagtaac
    21361 ccttcaacga cggggttact caagattgag gaaggattat cgttcggcga ccagacgaat
    21421 aatatcttcg tcttcaaccg atttttgact ctcaacggtc tttttatctg ctttctcagg
    21481 ttcgtttgct tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa
    21541 cgcctcaagc agttcaatct gttcgttagt ccgtgaactg gcacgattga taaaaaacca
    21601 caagatgagc ccgataagaa gaaccaccac cgatacaacc aaagacgcaa tgttcatcac
    21661 gcctgaattt acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact
    21721 gctggagagg aagaaaatcc agtgctgaaa aaatgatatc accacgggat aaactggtta
    21781 atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc
    21841 gcccaaagca gcaagcaagc caacaccacg acaaccagag cgacccatcg aaactttttc
    21901 actccaatct ccgtttcacc taccttatgt cattaaacta gcatgcgtta gttaaacagc
    21961 gcctaactat gtcgatattc gtgctttttc agaatgagtc gcttgcttca gcgcatattg
    22021 ccgctacgat taagcgaaca taaaaagaga agaggttgta atgcgattca tcattcgcac
    22081 agttatgctg atagcactgg tatggatagg attattactc agcggatatg gcgttttgat
    22141 tggaagcaaa gagaatgccg cgggattagg tttgcaatgt acctatctga ccgccagagg
    22201 caccagcacg gtacaatacc tgcacactaa aagtggtttt ctggggataa cagattgccc
    22261 gttgttacgc aaaagcaata ttgtggtcga taatggttaa gggtaataaa taaccagatt
    22321 ctcactcatg agtaaaatat tcttatcgca agtgcgttta atataacgcc accttcaact
    22381 ttatttttct catgctaaat aaagaacctg tctgtttgat attcagacag gttcttactc
    22441 tcatcagaac ggatagtcgt gataacccat ttgggcagaa attttgcgcg ccgcggtatg
    22501 caacattgcg acatattcct gtaaacgctc ttcagagaaa cgcaacgtcg ggaaggagat
    22561 gctcagaccg gcaatgacca cgccaaagcg atcaaatacc ggaaccgcaa tgcaacgcag
    22621 tccttcttcc tgctcttcgt tatcttcgcc atacccctgc tcgcgcacct ggtccagaac
    22681 gggtaataac gcttctgtac tgatgatggt ccgttcggta ctgcgtttat actccacgcc
    22741 ctcaagaatt tgcatcactt catcgcgatc gcgccatgcc agcagtacct taccaatcgc
    22801 ggtgctgtac agcggattac gacgcccaat ccgtgaatac atgcgcaaat tgtacattga
    22861 gtcaattttg tggatgtaaa caatactgtc ttcgtccagt gcgccgaggt ggatagtttc
    22921 tttggtcagg cgggagatct cacgcatctg gatatctgcg ctacgaatta aatcgacgtt
    22981 ttgcaacgcg cgggcgccca gttcaaacaa tttcagggtc agcgaatatt tctccgactc
    23041 cccttcctgc gccacataac ctaaggtttt catggtctgt aaaaagcgat aaacggtgct
    23101 ttttgacatc atgacgcgct gcgacaactc ggttatccct atttcgcgct cttcacccag
    23161 cgcctgcaaa atgccaaaaa cttttagcac ggaagataca gaatcaggct gtttatccag
    23221 atctgcgtta gccatttatc acctcattgc gagtgtttta taaaaatcag aactgttttt
    23281 t
//
LOCUS       DAOFSV010000062        23275 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.062, whole genome shotgun sequence.
ACCESSION   DAOFSV010000062 DAOFSV010000000
VERSION     DAOFSV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.062"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..615)
                     /gene="hipA"
                     /locus_tag="QBH27_002560"
     CDS             complement(<1..615)
                     /gene="hipA"
                     /locus_tag="QBH27_002560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDQ7014860.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYY"
     gene            complement(615..881)
                     /gene="hipB"
                     /locus_tag="QBH27_002561"
     CDS             complement(615..881)
                     /gene="hipB"
                     /locus_tag="QBH27_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDQ7014861.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            1189..1947
                     /gene="tam"
                     /locus_tag="QBH27_002562"
     CDS             1189..1947
                     /gene="tam"
                     /locus_tag="QBH27_002562"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDQ7014862.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     ITALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAIQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1951..2865)
                     /locus_tag="QBH27_002563"
     CDS             complement(1951..2865)
                     /locus_tag="QBH27_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDQ7014863.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIVFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(3062..4513)
                     /gene="uxaB"
                     /locus_tag="QBH27_002564"
     CDS             complement(3062..4513)
                     /gene="uxaB"
                     /locus_tag="QBH27_002564"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDQ7014864.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGVRNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(4740..6158)
                     /locus_tag="QBH27_002565"
     CDS             complement(4740..6158)
                     /locus_tag="QBH27_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ7014865.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDTLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(6297..6656)
                     /locus_tag="QBH27_002566"
     CDS             complement(6297..6656)
                     /locus_tag="QBH27_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDQ7014866.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(6656..7582)
                     /gene="glsB"
                     /locus_tag="QBH27_002567"
     CDS             complement(6656..7582)
                     /gene="glsB"
                     /locus_tag="QBH27_002567"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDQ7014867.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(7646..9034)
                     /gene="sad"
                     /locus_tag="QBH27_002568"
     CDS             complement(7646..9034)
                     /gene="sad"
                     /locus_tag="QBH27_002568"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDQ7014868.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLVAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEDNALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            9135..10016
                     /locus_tag="QBH27_002569"
     CDS             9135..10016
                     /locus_tag="QBH27_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7014869.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVESWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            10094..10177
                     /gene="yneK"
                     /locus_tag="QBH27_002570"
                     /pseudo
     CDS             10094..10177
                     /gene="yneK"
                     /locus_tag="QBH27_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            10355..11545
                     /gene="ydeA"
                     /locus_tag="QBH27_002571"
     CDS             10355..11545
                     /gene="ydeA"
                     /locus_tag="QBH27_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDQ7014870.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALLTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(11570..12235)
                     /gene="marC"
                     /locus_tag="QBH27_002572"
     CDS             complement(11570..12235)
                     /gene="marC"
                     /locus_tag="QBH27_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDQ7014871.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EVKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            12447..12881
                     /gene="marR"
                     /locus_tag="QBH27_002573"
     CDS             12447..12881
                     /gene="marR"
                     /locus_tag="QBH27_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDQ7014872.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12902..13285
                     /gene="marA"
                     /locus_tag="QBH27_002574"
     CDS             12902..13285
                     /gene="marA"
                     /locus_tag="QBH27_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDQ7014873.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            13317..13535
                     /gene="marB"
                     /locus_tag="QBH27_002575"
     CDS             13317..13535
                     /gene="marB"
                     /locus_tag="QBH27_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDQ7014874.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(13566..14465)
                     /gene="eamA"
                     /locus_tag="QBH27_002576"
     CDS             complement(13566..14465)
                     /gene="eamA"
                     /locus_tag="QBH27_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDQ7014875.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLTGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFAASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            14660..15847
                     /gene="ydeE"
                     /locus_tag="QBH27_002577"
     CDS             14660..15847
                     /gene="ydeE"
                     /locus_tag="QBH27_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDQ7014876.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTFCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            15974..16069
                     /gene="mgtS"
                     /locus_tag="QBH27_002578"
     CDS             15974..16069
                     /gene="mgtS"
                     /locus_tag="QBH27_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDQ7014877.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(16288..17178)
                     /gene="dgcZ"
                     /locus_tag="QBH27_002579"
     CDS             complement(16288..17178)
                     /gene="dgcZ"
                     /locus_tag="QBH27_002579"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDQ7014878.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(17432..17536)
                     /locus_tag="QBH27_002580"
                     /pseudo
     CDS             complement(17432..17536)
                     /locus_tag="QBH27_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
     gene            complement(17685..19730)
                     /gene="dcp"
                     /locus_tag="QBH27_002581"
     CDS             complement(17685..19730)
                     /gene="dcp"
                     /locus_tag="QBH27_002581"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDQ7014879.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWRRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGTAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            19867..20613
                     /gene="ydfG"
                     /locus_tag="QBH27_002582"
     CDS             19867..20613
                     /gene="ydfG"
                     /locus_tag="QBH27_002582"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDQ7014880.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            20702..21388
                     /gene="ydfH"
                     /locus_tag="QBH27_002583"
     CDS             20702..21388
                     /gene="ydfH"
                     /locus_tag="QBH27_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDQ7014881.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            21565..21768
                     /gene="ydfZ"
                     /locus_tag="QBH27_002584"
     CDS             21565..21768
                     /gene="ydfZ"
                     /locus_tag="QBH27_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDQ7014882.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(21804..23264)
                     /locus_tag="QBH27_002585"
     CDS             complement(21804..23264)
                     /locus_tag="QBH27_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ7014883.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGVE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPDNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         6015 a         5929 c         5441 g         5890 t
ORIGIN      
        1 gtaatactca ttatcaacgc tttggctaag atcgagcgtc gcattgggct gtctgatttc
       61 gccaatcggc aatttgataa tgtgcgtcgt tggcgttatt cccttcggaa tgcaccagtc
      121 attgcctatt ctgagcagcg cagtcttctc ctgtgcgcca gcaaccgaga tgcgaaagtc
      181 attttcttct ctaatcatgc ctaacgggat atctgcttta taagccgtta atacttcttc
      241 aagtctggct tcagtaagct tttcccatgc cattatcgga cacgttacgg tttcatcttc
      301 ggggattaac gtcacggcac caacgctgtc tcgccctatt tctgacaata aatcaaacgg
      361 ttgtctggat ttagcatgat aacgtttaac gatccggtca cgtacaatcg ggctatcggg
      421 taacaggtta tcgaagaagt taaatacggc atcagaggtg atattccccc tctgcaatgg
      481 cagcgaaagt gacaacggtc tggcataacg gctggctaac cactcagctg catacttaaa
      541 ggtgtgcgcg ccgttggcta acttcgttaa ctcgcctacc cgctggttgt tcatccaagt
      601 gactagttta ggcattacca ctccagatct tgctgttctg ttgcttctgg cgaggcattt
      661 ttcgcgtcgc atagcgtcat tgagagttca agcgactgta aaatcttaaa aaatgtcgtg
      721 agcgaggtat tgtcaggatt gttttcgaaa ttggaaattg tcgcctgctt aatgccgatt
      781 tttttcgcca actcgctctg cgtccagccg ttttgctggc gaaccagttt cattgcattc
      841 gccaattgcg ttgggctata gatcttctga aagctcatca tgtcatacgt ccacagcaaa
      901 tttatccgct taaggggata ttatcagttt tatcctttag tgaggataaa gtcaatctgg
      961 aaacacatct tatccgctct acgggataaa cgctgcgata tccgcgatcg cggataaaaa
     1021 ggaggattta aaaaagcaca tcctaatgcg gtggcttctt tgactaccgg aatctgtaat
     1081 cttaatgacc gggccgcgta aaaaacgtct gttcaatggg gtgatgccgt gaggcgaatt
     1141 tatcagtttt atctacaatt ggggtaacgc gctgacggga gtaaaaaaat gtctgactgg
     1201 aacccctctt tatatctgca ctttgccgct gaacgatcgc gtccggcggt ggagctgctt
     1261 gccagggtgc cgctggagaa tatcgaatac attgccgatc ttggctgtgg cccaggtaac
     1321 atcaccgccc ttctacacca acgttggcct gctgccagga taacgggcat cgactcatct
     1381 ccggcgatga ttgctgaagc gcgcagtgct ttgccagact gcctgtttgt ggaagcagat
     1441 atccgcaact ggcaaccgga acaggcactc gatctgattt ttgctaatgc ctcactgcaa
     1501 tggctgcccg accactacga attgtttcct catttggttt cgttacttaa ttcgcatggt
     1561 gtgttggcaa tacagatgcc agataactgg ctagagccaa cccatgtgct catgcgcgaa
     1621 gtcgcctggg aacaaaatta tccggatcgc gggcgtgagc cgctggctgg cgttcatgct
     1681 tactacgata ttctgagtga agccggatgt gaggtcgata tctggcgaac cacttactat
     1741 caccagatgc cgtcgcacca ggcgattatc gattgggtga ccgccactgg attacgtccg
     1801 tggttacagg atctgaccga gagcgaacag cagcattttc ttacgcgcta ccatcagatg
     1861 ctggaagagc aatatcccct ccaggagaac ggacagatat tgctggcatt tccgcgtctg
     1921 tttattgttg cccgccgtac ggagtaatta tcacgtcagc tggtaatggc gatcgggaag
     1981 aattttcgct ggaatttctg cttcatcatt catctgtaac aggtcaattt caatagcgtt
     2041 gcagatggca tccagcggta aatcattgtt ttcagtaccg aagggatctt ccagttcttc
     2101 cgccagacaa tccagcgaaa taaaagtgta ggaaatcagc acagagataa aaggcgtcat
     2161 gtaatgcagg tccacgacca gcgcaaacgg caacatgata caaaacagat acacggtgcg
     2221 atgcaaaatc agggtgtagg caaagggaat tggcgtatag gcaatgcgct cgcatcctgc
     2281 caggaccgct gaaatatcat taagccgatc gttgaggcta ataaacagga tatctgaaag
     2341 ttgtccattg cgtcgctgaa ccgccaacca ctcccccatt attaacaaga tgcggttagc
     2401 tggagagtta gaagccagta cacgctgaag atcttcagtc ttgagataat gagccagcac
     2461 ttctgcctgt ggctgtttgc gtaatgtcat gcgtaaacag tgggcgaaga cgatttgcag
     2521 ccgggcaaac tcccttacac ttgccgaatc cggtaatgtc gtttttacct cgcgcagtaa
     2581 cgaccgcgag gcaatcatca actgccccca aagttttcgc gcttcaacgt aacgggcgta
     2641 accggcatta ttacgaaaac caagaaaaat ggcgatggcg acaccgagaa tgctgaacgg
     2701 tgcgagggtg aatttaatgc ccaaatgcgt gtaccagggc agcatgaaaa taacagcgat
     2761 cgaaaagaga aaattgagta gtaagcgcga ggatatcttg gataatactg aaccgtgcca
     2821 gacaaaaata cggcgcagcc agtgttgttg tggacgaaca atcatggtta tcttcaggcg
     2881 tggaaaagtc gccctattaa acgtgattat gatcacattc tcaagatact acttacaaat
     2941 tacctacaag attacaacat taacaggtgc tgcatgtggc acctgttaat gataaaggca
     3001 tatagaatgt tgtaactaat atagcggaat aagaaacccg gtcgaaaccg ggttcagagt
     3061 attagcacaa cgggcgtaca gcttcgcgca tccctttttc gagaatcgca tccaggtcat
     3121 tagcaacctg ctcgaccaga ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt
     3181 ctgccaacac aatcgctacc agttcctggg tcccgataac acggtcacga tactggctcc
     3241 agagttgctg ataacgttcc agccagtgtg catcgtcctg taccggatat gtttctccat
     3301 tacgcacacc gcgatagaac gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa
     3361 gtgtgccgtt tgccttctgc cctgccagca gctgcggcag gatgcgtgtg cggaacttgg
     3421 tcataccgtt gagcgcgata gacagcagtt gatgcttgat gtacgggtta cggaaacgtc
     3481 cggtgactgc actggcgaaa gactccagct catcgcgtgg caaatccagt accggaataa
     3541 tttcttcgta gatggctttt tcaacgaatg cgcaaatttc agcatcgttc atcgcttcac
     3601 ctacggtatc cagccctgcc tggaacgcca ccggcaccag cgcggtgtgc gcaccgttga
     3661 ggattgccac tttgcgttct ttatacggtt taatatcgtc aacgatcagc acgttaagcg
     3721 gatatttgtc cagacgaagt tcagtcgcta aggatttcgg cccctgaatc acaaacaggt
     3781 aaaagtgttc agcggtgtcg aggaaaccat cgtggtagcc cagttcttct tccagtttag
     3841 ccacttcatc acgcggataa ccggtaacga tacggtccac cagcgtagaa cagaagctgt
     3901 tagcctgatc cagccattga ataaatgctt caggcaatgc ccactcttgc gcatagcgca
     3961 gcaccagttc acgcaacgcg tcgccattat agtcaatcaa ctcacacggg atgatgatcc
     4021 aacctttatc cagcgcaccg ttgaaatggc taaaacgttc gaacagcaga cgggtcagtt
     4081 ttgccggata acttaccgct ggcgcgtcat cgaatttatc gcccgcatgg tagctgatac
     4141 ccgcttcggt ggtgttagag aaaacaaagc gcatttccgg gttgtgagcc agtttcagga
     4201 attcatcgta ttcactatag acgctgattt cacgattaac cgagcgaatc agacgcgcgt
     4261 cgctgaccgc ttcacccttc tcattcaggc cacggataat ggtggtatac agaccatcct
     4321 gcgtgctcag tgacggcggg aatgaagttt caatcggacg aacaacgacc acgccagaat
     4381 tcagatcggt gtgctcattc aggagatcga tttgccagtc aacaaaggcg cgcaggaagt
     4441 taccttcacc aaactgaatg atacgttctg gatactgtgc accgggaaaa tcgcgacgat
     4501 ttagtgtttt cacaatgggt tcccttctga ttagtcatac aacctgtttg aattggtacg
     4561 acaggttagc aaactttaat acgtcgaacc cctgttttga tcaactcctg atgattaatg
     4621 agcagtttta tgagaaaagt gtggcgcgga tcatggttta atcgaggaaa caggcctttt
     4681 cttggatcat aaagtggtag aacacattgc atttaattcg ctctgagtaa ataaagatgt
     4741 cagacaactt cctcaccgta acgcatagtg ctggtacggt tgcgcccatc tttcttcgag
     4801 cgatacagac acgtatcggc ttcgaccatc aatttattaa agtcatcggt cagcgttcgg
     4861 tacgatgcgc aaccactacc gacgccaata cttaccgtga gataaagcgt tttttgttgc
     4921 caggtgaacg gttgcagttc aacgccttta cggatttttt ccgccattag caggccatcg
     4981 acaggattca cagacggcac tgcaacagca aattcttcgc cgcccattcg cgccaccagc
     5041 cccttatcac cgacaatctt ctgaatatgc tgggcaaaca cgcttaacac tttatcgcca
     5101 cattcatgtc catagttatc gttaatgctt ttgaaatagt cgatatcaag cagcatgacc
     5161 gtcagatgtt gtgtctgttt cagcgatgga cttttcagcg cctcataaag accggaccgt
     5221 gagtaaacct gggtcagaaa atcaaaatcg gctcgcagcg caacttgctt cattagcgaa
     5281 ttgatcgctg ccacgctaaa ggaaaccata attgggcata tcgccatcgt ggcaataccg
     5341 agacgtgcgg agaacatttg cggaatggag aacggcgaac cgaccgagat atcaatcacc
     5401 gaatttgcca ccagtacgac ttccacagcg ccggtgacaa aggtcaacag acatgttact
     5461 tgcggcgtat agcgcactgc acaccagatt aatgcaggga gcggaaacgc cagactcccc
     5521 gctccgccaa tgactaccga ggcaataacc gacacaataa gcgcgatagc aggcatcatc
     5581 tgctctgctt taaagcgcgg cagcactcca ggaattgcca acgtcagcat acaaggcacg
     5641 atcaacacgc ccgttgagaa ttgctcacta aaccagtcgg caagcaaagg ccagaaatcc
     5701 agagtatcaa tactgaccga accaatcgcc ccgacaatag cgcataataa tgcggcaagc
     5761 agacagtaat taaataaccg taaggcactt actggctcat acttattttt tcccagacgc
     5821 ttatcccgcg cgaccagtaa ggcgacggta acaataaaca tcatattgga gaaattgata
     5881 gccagtgaaa ccagccccca ttcggtggtt atggcatcat aaaccagcat cgccacataa
     5941 ctgatcgcat agtaatgcag tcgattaagc cagacatagc gggcaaacac ccccgccatt
     6001 acgccattca gcggccagaa aagagaaagt tcgtgaacca gccgtaactc tgcaccaata
     6061 aaatagaaca gcgtggttag cacaaaaata gcgatgctat tacgtagcag atgaccttcc
     6121 tggaacaaac ggaaagttga aatgggctgt acatgcatta attatctgtc catacaattg
     6181 cgggtggcaa ttatctcaac gcaataagta aattcttaga aaggatagca tgttcgctga
     6241 caacggcggc aactaaagat acctaatgaa tgccatttta tcgatttccg taattatcat
     6301 ggctgattca tctgtataac cagccagtga tagattaccg tcacaatata acgttgttgc
     6361 tcctcactta acgcttcccc ctgaggaatg ttatgccact tagccagaca accgcgacaa
     6421 caggtcgcgg tggcgtgttg agcgataaaa accggatgcc cgcgcatggg ggtttgctta
     6481 ccgtcgttag ccggtaatgc cggagcaaga cgtttagcga caaaatctgc cgcatgttgt
     6541 tcaatgactg gcgcgccttt ctccagacaa tactgacgct ctttcacacc caaacgaaag
     6601 cgagagcgaa attttgaacg ggataaacgc gcgaagagtg gatcgagaga ctgcattaat
     6661 aaactgaacg ccctaattgt ttcgtcaatt gttcaaggac ggcaatgccc gcaagcgagt
     6721 taccggcatc atccagttcc ggactccaga cagcgatggc catttcatgc ggaacaatcg
     6781 ccacaatacc gccaccaacg ccagattttg ccggtagtcc cacccgccag gcaaactccc
     6841 ccgcgttctg gtacataccg ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg
     6901 gcgtcaccac aggttcatca atatgaatag ctttcccctg attcgccaga aagacaaacg
     6961 tccgggccag ctctacacag ctcattttca gagcgcagta atgaaagtag ttttgcagaa
     7021 cggttgtcac gtcatgatgg aaattgccaa acgacttcat cagccaggcg atagccgcat
     7081 ttcgcgcgga atgttcaaat tcggaacgcg ctaccaccgt atcgtaggaa atatcagaca
     7141 caccacttaa gccgcgcacg acttccagca tacgttgccg aggtgcgctt aatcgccctt
     7201 gcaacatatc gcagaccacc agcgcaccgg cattaataaa cggattacgc ggaataccct
     7261 gctccatttc cagttgcact aaagaattga atggtgatcc agacggatct ttgccgacgc
     7321 gttgccagat ttcctcttcg gagtaatgac gcatggcgac aacgagactc agcactttgg
     7381 aaatagactg aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa
     7441 cggtacagat agcaatcccc aatcgggaac cgtctaccgt agccagcgcc ggaatataat
     7501 ccgcgacttt accctgacca atgagcggcc gcacttgccg caagatgttt tctaaaattg
     7561 cattatccat ggcgactgcc actttctact cctggaccgc aggtctgaaa agacctgcga
     7621 gtatatcaga gctgaatata tagcgtcaga cccggtcttt ccacaccgtc tggatattac
     7681 agaattcgtg taagccgaaa tgggaaagct cacgaccaaa gccactcttc ttcacgccac
     7741 caaaggcgac tcgcgcatcg ctggcacaat aaccattgat aaacacccca ccgcattcca
     7801 gacgtgccgc catctgtctg gcctgtgttt cgtcggtggt gaaaatggtc gctgaaaggc
     7861 cgaactcact atcattagcc agttccagcg catgttctgc atctttcgca atggtgattg
     7921 ccgcaacggg gccaaacatt tcttcccgaa acgcggtcat ttctggggta acattcgcca
     7981 gaaccgttgg cggatagtag ttacctgccc cagccatctt ttccccgccc agtaacaaac
     8041 gcgcaccctg cgccagtgtt ttttccacct gatgatgcag ctcatcacgt aaatcaaaac
     8101 gagccattgg tccgagagcg ttgtcttcat cacggggatc gcccattttc aaggctgccg
     8161 cagctgccac aaaacgttcg gtaaatgccg aagcaattcc ctcttcgatg ataaagcgtt
     8221 ttgccgctgc acaaacctgt ccggtattct gataacgtcc ggctaccgcc gctttcaccg
     8281 ccagttccag atcggcatcg ttaagcacaa taaacggatc cgaaccaccc agttccagta
     8341 cgcatttttt cagtgccgct ccagcctgtg cgccaatagc cgctccagca cgaacacttc
     8401 cggtcactgt gacagcagca atgcgcgaat ctttaatcat ctgactgaca ccgtcgttgt
     8461 cggcattcag ccagccatat acgccttgtg ggatacccgc atctttaaac acctgggcaa
     8521 cgagctgtgc acagcccatc acattcggcg catgtttaag taagtagccg ttaccagcaa
     8581 gaatgatggg aacagcgcca cgcatcacct gccataacgg aaaattccac ggcataatcg
     8641 ccagaatcgt ccccaacggt cgatactcaa taaccgcctg ctgattttcc accagcgtag
     8701 gttctgcctt cagcattgcc ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt
     8761 tcgccacttc agcgcgcgcc tggttgattg gtttgcccat ttcacgggtg atcatttgcg
     8821 ccatttcttc gctacgggcg cgcagagcct taccgatatc acgcagtttt tcagcacgat
     8881 aatctatatt tgtctcgcgc cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt
     8941 cgatatcgtc aacaccagcc cacggcagca cagaaagttg ttcaccggtg gcaggattta
     9001 tcgaaattgc atgagttgcc ggagtaatgg tcatcggggt atctccttta tgagtcatgg
     9061 tatgaagata cgcagattta ctcttgcttt aaaatgaata atattaagcc acttattcac
     9121 gaatcgagaa tgccatggat ctgacccaac tggagatgtt caacgcggtt gccgaagctg
     9181 gcagcattac ccaggctgca gcaaaagtgc atcgcgtgcc gtcgaatctc actacccgtc
     9241 tacgtcagct ggaaacagaa ctgggggttg atctgtttat tcgcgagaat cagcgtttac
     9301 gtctctcgcc tgccgggcat aactttttac gctatagcca acaaattctc gcattagtgg
     9361 atgaagcgcg gagcgttgtc gctggcgatg agccgcaagg tttattttct ctgggatcgc
     9421 tggaaagcac cgctgcagtg cgcattccgg ccacgctggc ggaatttaac cgtcgttatc
     9481 ccaaaattca gttttcactt tccaccggcc cttccggcac tatgctggaa ggtgtgctgg
     9541 agggaaaact gaatgcggcg tttattgatg gacccattaa ccatactgcc atcgacggga
     9601 taccggtata ccgcgaggaa ctgatgatcg tcacgccaca aggacatgcg ccagtaaccc
     9661 gtgccagtca ggtgaatggc agtaacattt atgccttccg cgccaattgt tcgtatcgtc
     9721 gccacttcga aagctggttt catgctgacg gtgccgctcc gggaactatt cacgagatgg
     9781 agtcttatca cgggatgttg gcctgtgtgg tcgcaggagc aggcattgcg cttattccac
     9841 gctctatgct ggaaagtatg ccggggcatc accaggttga atcgtggccg ttagctgagc
     9901 aatggcgttg gttaacaacc tggctggtct ggcgtcgtgg tgcgaaaacg cgtccgcttg
     9961 aggcatttat tcaactgctg gatgcgcctg actcggcaaa acagggttac caatgagcta
    10021 tttttgatag ttcatgcggc taatatgctc tatatagtga tgttccaatg acttatgact
    10081 atatggggca aatatggtta cgccagtaag catcagcaat tacatatctc ttcctgatga
    10141 ttttcccgtt cgtaatattg cacctcaagt aaaagaagaa caggccccct tcctgatcgt
    10201 cattaaaatg tgatgttaac aactctctga tgcgtgacag aacaaccgtc agagagatgg
    10261 aaatgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt
    10321 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
    10381 agtagcgtgg ctacgggtcg ttacgctggc agtcgccgcc tttatcttca ataccaccga
    10441 atttgtccct gttggcctgc tctctgacat tgcgcatagt tttcacatgc aaaccgctca
    10501 ggtcggcatc atgttgacaa tttatgcatg ggtagtggcg ctaatgtcat tgccttttat
    10561 gttaatgacc agccaggttg aacgccgcaa attactgatc tgcctgtttg tggtgtttat
    10621 tgccagccac gtactgtcgt ttttatcgtg gagttttacc gttctggtga tcagtcgcat
    10681 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
    10741 ggctccggcc gggaagcgag cacaggcatt gagtttaatt gccaccggta cagcactggc
    10801 aatggtatta ggtttacctc tcgggcgcat tgtggggcag tattttggct ggcgaatgac
    10861 cttcttcgcg attggtatcg gggcgcttct tacccttttg tgcctgatta agttacttcc
    10921 cttgctgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
    10981 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
    11041 cagctatatc gagccttttg tacaaaacat tgcgggattc agcgccaact ttgccacggc
    11101 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
    11161 taatcagtat gcgtctgcgt tggtgagtac agcgattgcg ctattgctgg tgtgcctggc
    11221 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
    11281 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
    11341 tgccaccgac gtcgcgatgg cgctattctc cgggatattt aatattggaa tcggcgcggg
    11401 tgcgttggta ggtaatcagg tgagtctgca ctggtcaatg tcgatgattg gttatgtggg
    11461 cgcggtgcct gcttttgctg cgttaatatg gtcaatcatc atttttcgcc gctggccagt
    11521 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatgatacg
    11581 ttttaatgat ttccaggatg ccgttaataa taaactgcac gcccatacat accagcagga
    11641 atcccatcag acgggagatc gcttcaatac cacccttgcc caccagccgc ataattgcgc
    11701 cagagctgcg tagacttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
    11761 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggag gccgagctaa
    11821 taatcatcgc aatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaagcaa
    11881 tattggcact gggttcatct tccagctctt ccgacttgct tttcacttcc ggtgaatcaa
    11941 tcgctttctg ttgcggaaag agcatccgaa aaccgataaa cgcgacgatc aaaccgcctg
    12001 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccggcgtaat
    12061 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
    12121 cggcactgtt catgttgcct gccaggccaa gaaataacgc gacagttgtt aatgggttag
    12181 ctaacggcag taacaccacc agccccaggc ctattgcttt aaacaaatct aacattggtg
    12241 gttgttatcc tgtgtatctg gtttatcagc gaaaagtata aggggtaaac aaggataaag
    12301 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
    12361 gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt atcccctgca
    12421 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
    12481 ttccattggg tcgtttaatc catatggtta atcagaagaa agatcgcctg cttaacgagt
    12541 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
    12601 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggggcactga
    12661 cccgtatgct ggatcgcctg gtctgtaaag gctgggtgga aaggttgccg aacccgaatg
    12721 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgt gaacaatgcc
    12781 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
    12841 tggcaacact tgagcatttg cttaagaaag tcctgccgta aacgaaaaaa gaggtatgac
    12901 gatgtccaga cgcaatactg acgctattac cattcatagc attttggact ggatcgagga
    12961 caacctggaa tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg
    13021 gcacctgcaa cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgtag
    13081 ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct
    13141 ggcagaacga tatggcttcg agtcccaaca aactctgacc cgaaccttca aaaattactt
    13201 tgatgttccg ccgcataaat accggatgac caatatgcag ggtgaatcgc gctttttaca
    13261 tccattaaat cattacaaca actagttgat aacgtgacaa cgtcactgag gcaatcatga
    13321 aaccactttt atccgcaata gcaactgcgc ttattctctt ttctgcgcag ggcgttgcgg
    13381 aacaaaccac gcagccggtt gttacttcct gtggcaatgt cgtggttgtt cccccatcgc
    13441 aggaacaacc accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc
    13501 tcggcgtgcc ctattataat caacacgcta tgtagtttgt tctggccccg acatctcggg
    13561 gcttattaac ttcccacttt taccgcttta cgccaccgca agccaaatac attgatatac
    13621 agcccggtca taatgagcac cgcaccgaaa aattgcagac ccgttaagcg ttcatccagc
    13681 aatagtgccg cacttgccag tccaactaca ggcaccagta acgataacgg tgcaacccgc
    13741 caggtttcat agcgtccaag cagtgttccc cagatcccat aaccaacaat tgtcgctaca
    13801 aatgccagat acatcagaga caagatggtg gtcatatcga tagtaaccag actgtgaatc
    13861 atggttgcgg aaccatcgag aatcagcgag gcagcaaaga agggaatgat tgggattaat
    13921 gcgctccaga ttaccagcga catcaccgcc ggacgcgttg cgtgcgacat gatcttttta
    13981 ttgaagatgt tgccacacgc ccaactaaat gctgccgcca gggtcaacat aaagccgagc
    14041 atcgccacat gctgaccgtt cagactatct tcgattaaca ccagtacgcc aaaaatcgct
    14101 aaggcgatcc ccgtcaattg tttgccatgc agtcgctcac cgaaagtaaa cgcgccaagc
    14161 acgatagtaa aaaacgcctg tgcctgtaac accagcgaag ccagtccagc aggcatacca
    14221 aagttaatgg cgcagaacag aaaagcaaac tgcgcaaaac tgatggttaa tccatacccc
    14281 agcagcaaat tcagtggtac tttcggtcgt gcgacaaaaa agatggccgg gaaagcaacc
    14341 agcataaagc gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg
    14401 accacaaaat ttagccccca tacaaccact accagtagcg ccaacacccc atcttttcgc
    14461 gacattctac cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt
    14521 ttctttaaca gttgattcgt tagtcaccgt ttacgacggc attaatgcgc aaataagtcg
    14581 ctatacttcg gatttttgcc atgctattcc tttacatctc taaaacaaaa cataacgaaa
    14641 cgcactgccg gacagacaaa tgaacttatc cctacgacgc tctaccagcg cccttcttgc
    14701 ctcgtcgttg ttattaacca tcggacgcgg cgctacgctg ccgtttatga ccatttactt
    14761 gagtcgccag tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac
    14821 tattggcgtc gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg
    14881 ctatatgtta ctggcaatta ccgccttcgc tagcggtttt attgccattc ctttagtgaa
    14941 taacgtgacg ctggtggtgc tcttttttgc ccttattaac tgcgcctact ccgtttttgc
    15001 taccgtgctg aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa cgaagatttt
    15061 ctcaatcaac tacaccatgc tcaacattgg ctggaccatc ggtccgccac tcggcacgct
    15121 gttggtgatg cagagcatca atctgccctt ctggctggca gctatctgtt ccgcgtttcc
    15181 catgcttttc attcaaattt gggtaaagcg cagcgagaaa atcatcgcca cggaaacagg
    15241 cagtgtctgg tcgccgaaag ttttattaca agataaagca ctgttgtggt ttacctgctc
    15301 tggctttctg gcttcttttg ttagcggcgc atttgcttca tgcatttcac aatatgtgat
    15361 ggtgattgct gatggtgatt ttgccgaaaa ggtggtcgcg gttgttcttc cggtgaatgc
    15421 tgccatggta gttacgttgc aatattccgt aggccgccgc cttaacccgg ctaacatccg
    15481 cgcgctgatg acagcaggca ccttctgttt cgtcatcggg ctggtcggtt ttattttttc
    15541 cggcaacagc ctgctaatgt ggggtatgtc agctgcggta tttactgtcg gtgaaatcat
    15601 ttatgcgccg ggcgagtata tgttgattga ccatattgcg ccgccaggaa tgaaagccag
    15661 ctatttttcc gcccagtctt taggctggct tggtgctgcg attaacccat tagtgagtgg
    15721 catagtactt accagtctgc cgcctttctc gctgtttatc atcttagcgt tggtgatcgt
    15781 tgttgcgtgg gtgctgatgt taaaaggaat tcgtgcaaga ccgtgggggc agcccgcgct
    15841 ttgttgattt aagtcgaaca caataagaat ttaattcagc cttcgtttag gttacctctg
    15901 ctaatatctt tctcattgag atgaaaatta aggtaagcga ggaaacacac cacaccataa
    15961 acggaggcaa ataatgctgg gtaatatgaa tgtttttatg gccgtactgg gaataatttt
    16021 attttctggg tttctggccg cgtatttcag ccacaaatgg gatgactaat gaacggagat
    16081 aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga tttttatgac
    16141 ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga tttgtgtttt
    16201 gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac agtagcatca
    16261 gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt gttcgtcaat
    16321 aaacatgcag cgattccttc cggtttgctt accctcatac attgccctgt ccgctcttcc
    16381 aatgaccaca tccagaggct cttcaggaaa tgcacgactc acaccagcag tcacggtaat
    16441 gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc aaattctgac
    16501 acctgcacga catgcttctt catcattagt cgctttgaca ataatgataa attcttcgcc
    16561 cccgtagcga taaaccgttt cgtaatcgcg cgtccaactg gctaagtaag ttgcgagggt
    16621 gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt taaatcggtc
    16681 tatatccaac aacattaaat aaagattcag aggttcagca ttgcgtaact gatgatcgaa
    16741 ggattcatca agaacccgac gacccggcaa tcccgtcaga acatccatat tgctacggac
    16801 cgtaagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca acccttcctg
    16861 aaaagcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca ttaattcccg
    16921 accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag gtaattcatc
    16981 gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc cataagaatg
    17041 gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa acatacttac
    17101 cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg catcaatttc
    17161 cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg ctattctaac
    17221 gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg agcatctctg
    17281 agataacatt gtgatttaaa acaaaatcag cagataaaaa agtgtttaat tctataaatt
    17341 aactctgcat tatcgcaatt aaaaggatga caaatagcat aacccaatac cctaatggcc
    17401 cagtagttca ggccatcagg ctaatttatt tttatttctg caaatgagtg acccgaacga
    17461 cggccggcgc gcttttctta tccagactgc cgctaatgtt gatcatctgg tccggctgaa
    17521 tttgaaaatt attataacgg aagagtatac aaaaaaagta attttgctgg cgactgcgaa
    17581 acggcattcg cattagcggt gaagtttgcc ctcgcccagc ggctgcaatt actgctataa
    17641 cgaggctggt ctggcgataa atccaggcca gccattggtg gtgtttatac gttcaagcca
    17701 cgatgttgca acatcggcat aatctgaggt gccttaccgc gccattgtcg gtacaggcgt
    17761 tccagatctt cgctgttacc tctggaaaga atcgcctcgc gaaaacgccg cccattttca
    17821 cgcgttaatc cgccctgctc aacaaaccac tggtaaccat catcggccaa catttgcgtc
    17881 cacagataag cgtaataacc tgcggcatat ccgccaccaa aaatatgggc gaaataactg
    17941 ctgcgatagc gtggcggtat agcaggaaga tccatatttt ccgccaccag cgcccgcaat
    18001 tcgaaatcat cgacatcctg cattgcttcg ttttcttcca ggcaatgcca gcgcatatcg
    18061 agaagtgcgg cgctaagcag ttcgctcatc tcataccctt tattgaacag gctggcatta
    18121 cgcattttct gttgaagttc gtcaggcatt gcggtcccgc tctgataatg ccgggcgtag
    18181 cgagcgaata cctgcggatg tgttgcccag tgttcgttga tttgcgacgg aaattcgaca
    18241 aaatcacgcg gcgtgttggt gccggaaagc gtggcataac gctggcgggc aaaaaggccg
    18301 tgcagcgtat gaccaaattc atggaataag gttattacat catcccagag taacaacgca
    18361 ggctcaccgg cagcgggttt ctgataattg cagacgttat aaattaccgg gtgcgtttca
    18421 ttcagcgttg attgctcaac aaaattgccc atccatgcgc cgccgctttt tgaatcacgg
    18481 gcgaagaaat caccgtaaaa taacgccagc cccacgccat tatgatcaaa aatttcccac
    18541 acacgaacgt caggatggta gacaggaata tcaaaacgtt cgacaaactt aataccgaag
    18601 agctgattcg cagtccagaa tacgccttca ttcaacaccg tgtttaattc aaaatatggc
    18661 ttgagctgcg cttcatcgag atcgaatttc tcacgtctta cctgctcggc ataaaatgcc
    18721 cagtcccacg gctgcgcgct aaacccgccc tgctgcttat cgataaccgc ctgtatggag
    18781 gctaattcat cgctcgcacg ttgacgcgcc gctggaacaa tttcccgcat aaagttgagc
    18841 gctgcttctg gcgtttttgc catctgatcg gcgattttcc atgcggcata atgaggaaaa
    18901 ccaagcagtt tcgcctgctg cgcgcggatc tccaccagac gttgaatgat agcgcgggta
    18961 tcattggcat catttttttc cgctcgcctc cagcccgcag taaacagttt ttcacgcgtc
    19021 gcgcgatcgc gcagttcagc aagcgctggt tgctgggtgg tattcagcag cggaatcagc
    19081 cagttgttat ccagaccttt ctcgcgagcc gcctctgccg ccagcgcaat ctcttgttca
    19141 ctcattcctg ccagctgcgc gatatcgttc acaaccagac cgccggattt atttgctgcc
    19201 agtaaccgct ggttaaactg gctggtcagg gtcgcagctt ctgtattcag tacttttaat
    19261 tttgctttat cagcttgctc aagtttggct ccggcaagga caaaacgttg atgaatcacc
    19321 tccaccaggc ggatggattc actatcaagc cccagggatt cacggcgttg ccagacagca
    19381 tctacccgcg cgaataattc cccgttcaga tagatatcat tcgcaagttc cgccagttca
    19441 gcggaaaact gttcatcaag acgctgtaat tcatcattgg tatgcgccgc agtcatcgca
    19501 aaaaagacgc tggtaacgcg ggtaagtaat tctccgcttt gttccagtgc cagaatagta
    19561 ttgttgaaat caggtgtttg cgggttaagc gcgatggcag caatttctgc ccgcttttgc
    19621 tgcattccct catcgaatgc cgggcgatag tgatgattgg caatttgatc aaaatgggga
    19681 gccagatacg gcagtgtgct ttgcacaagg aaaggattca ttgttgtcat tttcttctcc
    19741 tgaacgcgag gtgttccata gcgtaggctt actgataacg gagtgcaatc ttgcaaacca
    19801 gtattacccg ctcttaagca tcacgtgcta tgttattgac acacaaaatc gttgaggaac
    19861 agtgagatga tcgttttagt aactggagca acggcaggtt ttggtgaatg cattactcgt
    19921 cgttttattc aacaagggca taaagttatc gccactggtc gtcgccagga gcggttgcag
    19981 gagttaaaag acgaactggg agataatctg tatatcgccc aactggacgt tcgcaaccgc
    20041 gccgctattg aagagatgct ggcatcgctc cctgccgagt ggagcaatat tgatatcctg
    20101 gtaaacaatg ccggcctggc gttgggcatg gagcctgctc ataaagccag cattgaagac
    20161 tgggaaacga tgattgatac caacaacaaa ggcctggtat atatgacgcg cgccgtctta
    20221 ccgggtatgg ttgaacgtaa tcatggtcat attattaaca ttggctcaac ggcaggtagc
    20281 tggccgtatg ccggtggtaa cgtttacggt gcgacgaaag cgtttgttcg tcaatttagc
    20341 ctgaatctgc gtacggacct gcatggtacg gcggtgcgcg tcaccgacat cgaaccgggt
    20401 ctggtgggtg gcaccgagtt ttccaatgtc cgctttaaag gcgatgacgg taaagcggaa
    20461 aaaacctatc aaaataccgt tgcattgacg ccagaagatg tcagcgaagc cgtctggtgg
    20521 gtgtcaacgc tgcctgctca cgtcaatatc aatacactgg aaatgatgcc ggttacccaa
    20581 agctatgccg gactgaatgt ccaccgtcag taatttttat acccggcgta actgccgggt
    20641 tattgcttgt cacaaaaaag tggtagactc atgcagttaa ctcactcaca agcaagaacg
    20701 aatgaccgtc gaaacgcaac ttaatcccac acagcctgtc aatcagcaga tttatcgtat
    20761 tcttcgccgc gacattgtcc attgcctgat tgcaccaggc acaccgttgt cggaaaaaga
    20821 agtttctgtt cgtttcaatg tgtcacgcca gccggttcgt gaagccttta ttaaactggc
    20881 ggaaaacggt ctgattcaaa ttcgtccgca gcgtggcagc tacgtcaaca aaatttccat
    20941 ggcccaggtg cgcaacggca gttttatccg tcaggccatt gagtgcgcgg tggcgcgtcg
    21001 ggcggcgagc atgattaccg aaagccagtg ctaccaactg gaacaaaatc ttcaccagca
    21061 acgcattgcc attgagcgca agcaactgga tgattttttt gaacttgatg acaacttcca
    21121 tcaactcctg acgcagattg ccgactgtca actggcgtgg gataccattg agaacctgaa
    21181 agcgaccgtt gatcgcgtgc gctatatgag cttcgaccac gtttctccac cagaaatgct
    21241 gttacgccag catcttgata ttttctctgc cctgcaaaaa cgtgatggcg atgcggtaga
    21301 acgtgcaatg acgcaacatt tgcaggaaat cagcgaatcc gtgcgccaga tccgccagga
    21361 aaacagcgac tggtttagcg aagagtaatt catttcctct catcccatcc ggggtgagag
    21421 tctttccccc cgacttatgg ctcatgcatg catcaaaaaa gatgtgagct tgatcaaaaa
    21481 caaaaaatat ttcactcgac aggagtattt atattgcgcc cgttacgtgg gcttcgactg
    21541 taaatcagaa aggagaaaac acctatgacg acctacgatc gtaaccgtaa cgcaatcacc
    21601 actggcagcc gtgttatggt tagcggcacc ggtcacactg gcaagatcct gtcgattgat
    21661 actgaaggtc tgaccgctga gcaaatccgc cgcggaaaaa ccgtagttgt tgaaggttgt
    21721 gaagagaaac tggcaccact ggacctgatt cgtctcggca tgaactaagc gtgtgaatgc
    21781 cgccgatggc ggcattgctt tttttacttc acggaatatt tcgccactgt cgctttcgcg
    21841 ccatgcgcta ataaagacaa gtacgcttcc gtcactttag tcgtaaacaa gctattatct
    21901 ggcaaatcat caccaaatat cgccttaatc gccaacaatg actgcacgcg cgctgtccct
    21961 tcagcactac tttgtactgc cttctgaata acaggtaaca gtgggtcact gatttctatc
    22021 ggatttccct gttcatcaac accaccgaca taacgcatcc aacccgcgac gcccagcgcc
    22081 agcagatcga acttgctgtc atgcgccaga tgccagcgaa cagaatccaa catccgctgt
    22141 ggcaatttct ggctgccatc catcgcaatc tgccaggttc gatgacgtaa cgccgggttg
    22201 ctatagcgtt caattaatcg gttagcgtaa tcttgcaaat caacgccctg cactttcagc
    22261 gtcggcgctt gttcctgcaa cattaaggta tacgccgcat gacgataatg ttcatcctcc
    22321 atgcagtcat taatgtgctg atatccggca agatagccca gatacgccag gaatgaatga
    22381 ctgccattga gcatgcgcaa cttcatctct tcataaggca gcacatcact aaccagttcg
    22441 actcccgctt tttcccattc cggacgtccg gcaacaaagt tatcttctat tacccactgg
    22501 cggaaaggtt cacaggcaac gcctgccgga tcgcgcacac cggtaagttg ttcgattttc
    22561 gccagcgtat cctctgtcac tgcgggcaca atacggtcca ccattgttga tgggaaagtc
    22621 acgttatctt cgatccattg tgccagtttt acatcaatgg cttgcgcata ggaagtgaca
    22681 acgtcacgca taacatgacc gttttctggc atgttgtcac atgacatgac ggtaaatgcg
    22741 ggaagtcctg ccgctttacg gcgagccagc gcctcaacaa tcacccctgt tgcagttttc
    22801 ggctggtggg gattttgcac gtcggcaacg accatcgggt gatcgagcat taactgtccg
    22861 gtcgccggag agtggaaata ccctttttcg gtgattgtca gagagacaat cgcgatttgc
    22921 ggttcacaca tcgcagccaa cacggtttct aagccgtcta tttgtacgtg taaggctttt
    22981 ttaacgacgc caacgacgcg agccgtccac gcatcagccg acatttccgc aacggtataa
    23041 agattatctt gctgttgtaa atcggcaatt tgctgttcgc cgccgattaa gttgacctca
    23101 taatatcccc agtcactgaa atgttccgta gcaagaatat cggcatacac gccctgatgc
    23161 gcacggtgaa atgcaccaaa gcctaaatga acaattcttg gggccaggtt attacgatca
    23221 taaacaggga gcgtcgcttt tgctgataac aaatgatttc ccataacaat tcctt
//
LOCUS       DAOFSV010000063        23046 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.063, whole genome shotgun sequence.
ACCESSION   DAOFSV010000063 DAOFSV010000000
VERSION     DAOFSV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.063"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(176..874)
                     /locus_tag="QBH27_002586"
     CDS             complement(176..874)
                     /locus_tag="QBH27_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogenic protein"
                     /protein_id="HDQ7014884.1"
                     /translation="MGTALISSFISGAIAIISIAISTYNQNRINKLNESLDVRRKHQY
                     YIEPLIRSASDLQSRIYNILELGFIEEFYHNGNKRQQDYVINNTVFLFSQFFAWTEAA
                     RIDIQYLSLEKNKKMREFIRLQNNINSLIQTDVFGQYFMFFIGEQRAIAEKMLISTDT
                     GFDCIGYGSFTKENCFINEPFFLDLNNEVINMTRDIGIYKERLIRIQHALIDLINFLD
                     PGMIRFDGKKYGKI"
     gene            2063..2986
                     /locus_tag="QBH27_002587"
     CDS             2063..2986
                     /locus_tag="QBH27_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HDQ7014885.1"
                     /translation="MRLKYSVLLLAIALTGCDNKKDEIGCSSEITQSALMDLLKKSAY
                     EGLSEQVDKYPDVTNQTKRSTLDKIKLAISEISTTSSDTGSTMKTCEGTVTMTLSANE
                     YVQLSDAYRKNFNRNLDKQMESLFLENNANAFSKRITYTVQATDDQKNVFVKTSTDNP
                     ISVGAALLTSLSIITPIVEQQKIQQAKDAQQSQIEAEQQVQLRAQEQAKYEAEKQIER
                     QAQLQPQKADNLDQSRMAFANADSDLSTVWSKLTPAKKKDLLPSQRQWIKTKDAMCGK
                     VSMQGTDAEVKKMVDCQTQMTLSRIAFLRNQ"
     gene            complement(3161..3949)
                     /locus_tag="QBH27_002588"
     CDS             complement(3161..3949)
                     /locus_tag="QBH27_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDQ7014886.1"
                     /translation="MTVQISAESLSPLHHNQLPVITTELLASLYGTEVNGIQQNFKRN
                     IARFVVGKHFFKLEGNELRDFKNRLTDSQSVAKHTRSLILWTERGAARHAKMLETEQA
                     WEVFEKLEDCYFSQRPERRSTTTDDRTPLRDAVNILVGKKGMMYPDAYTMIHQRFAVS
                     HIEQLSQTQMLEAIEYIHCLLLDENHPTIPDFSFITTIKNGKVTGMRHVAKGEHLMTF
                     DAFKEIAERAGYLVIHSDNLRSMTLDKLMVMGKKSAISVANSAF"
     gene            complement(4631..4855)
                     /locus_tag="QBH27_002589"
     CDS             complement(4631..4855)
                     /locus_tag="QBH27_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014887.1"
                     /translation="MKELFLAFVPRFINDQIALTDNGEQYEIACSMVDVNPGERYDAM
                     CDLKIFTWLGWAIPCGEPTNIRPFESREAV"
     gene            complement(4852..5163)
                     /locus_tag="QBH27_002590"
     CDS             complement(4852..5163)
                     /locus_tag="QBH27_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ7014888.1"
                     /translation="MKYKRWVRAEVVIIKQCAGSMTVERIGQLIGRTGAAVRTKAREL
                     KICMYLRGNYHQSVKYLQEDIELARELHQSGINRQDIAEKLEMPIGAVNQFVYFERRI
                     S"
     gene            complement(5160..5396)
                     /locus_tag="QBH27_002591"
     CDS             complement(5160..5396)
                     /locus_tag="QBH27_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014889.1"
                     /translation="MAGQSDYLPPGLPHNRAKWPQEYQLKEHYDMRAAALIRQLFEKR
                     IPRGSVIEQIGMTPDTYREFFRERLNYWRGVMEQ"
     gene            complement(5398..5808)
                     /locus_tag="QBH27_002592"
     CDS             complement(5398..5808)
                     /locus_tag="QBH27_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014890.1"
                     /translation="MTTTPGKLEYPSDKGHIDDGKNYLDVILWNMNVGPRARTRAVFV
                     PRPKAGNFSTPAKPARQASVAAAPAVKRKGKTHTGIAIRRNGERQVKLHETATTWCAS
                     PHETYDKITGQRIGAPGRCRLLLSSITPIAKKGA"
     gene            complement(5847..7262)
                     /locus_tag="QBH27_002593"
     CDS             complement(5847..7262)
                     /locus_tag="QBH27_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ7014891.1"
                     /translation="MINNAIALYSVDAEQAVLGCLMLNTDHDRTNAVFALLKPETFYI
                     NAHQVIYREIKGLFQADKPTDLITLTELMESKGLYERVGGFAYLAEMSKAAIPASMVN
                     YAKIVREKAILRYAVEKLNSCLEIMIQPADMTATDRITAVQQVIGQVAEHSRTGHKGG
                     LRPASEVVDDWIDDLERRFNDPAHAAGLTLGIESLDRLMAPKQALRGSLVVIGARPKM
                     GKTAAFNKIAVHFALNHRLPTLVFSLEMTDRSLIERMVAQEAKVNSEIFYLGPNDESE
                     LSLAIAKAGEIAESNMMIDSTAGVTLAHIVAECRKVKRMRGSVGLVAIDYLTLMKTES
                     AERRDIAYGDITTGLKNLAKELDCVVVLLTQLNRKLEERADKRPNPSDSKDTGQIEQD
                     CDVWIGLYRDAVYNENADPNLMEMLLRLNREGPSGTAYALMKNGSVIDISDEEVYRLT
                     NDRSEKNKRYSRSDNSRTNEF"
     gene            complement(7252..8007)
                     /locus_tag="QBH27_002594"
     CDS             complement(7252..8007)
                     /locus_tag="QBH27_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ7014892.1"
                     /translation="MNILPLLDRPIAFQRSFIRLEMGVTAALFLSQLTYWTNRTTDDG
                     WVYKTQDEWEEETGLSRYEQEGARKKLRGLGVLLERKQGLPARLYYKIDNDVLCQLLT
                     SAYKDAEKPHTGEGKTTRPVRGKPTNILTENTTEIITDGESADADTQQPADQRINYQV
                     ILDTYHEILPEMPPVKILTDGRKKHLRTFWKKFNFNESRWRAYLEFIGGNCRWMLEDR
                     PNGRGGFWRRKNLDYLITERCYVSVKEERANDK"
     gene            complement(8004..8228)
                     /locus_tag="QBH27_002595"
     CDS             complement(8004..8228)
                     /locus_tag="QBH27_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014893.1"
                     /translation="MLRHIDRITWRNGWHLNGRPAHVAEIRPIFDGRVAAARSVWEKY
                     EEEKAKLREQNLSGAAYEAGCRVLSEALGI"
     gene            complement(8268..8744)
                     /locus_tag="QBH27_002596"
     CDS             complement(8268..8744)
                     /locus_tag="QBH27_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014894.1"
                     /translation="MENQEELQKEILTWAARAGQELVTIEICRAWFSQGRNDELRLHE
                     FEDADGNVDWRAINNNRQKIFRWLRGETTAARRKTQVLASVMKAVLPAERRARLESPG
                     DPVLLATLAAKEGVEAINAVHLHFAPELTIQEIDEAIAALVATRGAVIRTAQDHHA"
     gene            complement(8803..9033)
                     /locus_tag="QBH27_002597"
     CDS             complement(8803..9033)
                     /locus_tag="QBH27_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7014895.1"
                     /translation="MDKQLQDKILSVMSQSELGRRLGKKPQTVSLWFKGRVPGEEVLR
                     TSEALEWRVTPHDLRPDLYPNPDDSLPKTEAA"
     gene            9132..9545
                     /locus_tag="QBH27_002598"
     CDS             9132..9545
                     /locus_tag="QBH27_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7014896.1"
                     /translation="MNTKTKNMAFASRLQRILKDLGWSQSELARHIGVTAQSVQAWCN
                     GVTPRKDKLDKLAKVTGYPVHFFFMNEGEYLDESTLHPGNYNQELTPQEQALLQLFRG
                     LPESEKNKLINELKEKREHFDLLLKELLEAKNQTK"
     gene            10556..10876
                     /locus_tag="QBH27_002599"
     CDS             10556..10876
                     /locus_tag="QBH27_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014897.1"
                     /translation="MEARTIPVTLFIHYATSTFSHEKLLVATVDMSKNFPDRYILLES
                     REIEITVNQPEPIDIIGLQVEQLREQKQKTVADAQQRIAAIDDKIQQLLCIEYTPDTD
                     ELPY"
     gene            10907..13123
                     /locus_tag="QBH27_002600"
     CDS             10907..13123
                     /locus_tag="QBH27_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014898.1"
                     /translation="MTIFNGLLEAKKGALKNGAIPALAIAIAAPNKKVAENIIIGKLW
                     EAYPDHGDNYFKPKIWEDAPGQPRPGVGEFDETFATEHSFDGEKWVVNTPVDSDCSSA
                     DISQVSDLMKLPARERFAAVLLFSHDANEVDSELLVQTREYLEMLDNSDTDSEDEVDA
                     FNRIVLDAMVACKPIEYMHIAGLNNLVHAILASCDTQEQNPTSWTISKFIKKWVENPG
                     KRDEMLPEVKPETASARPYKQTHATLDREIACALLPVAPEKITPSILKAADEIISQDR
                     EDFKRWSMALRTTDQILAYDRASVFGVIQSAPAKDTYHFPQSLRSHIDNWLQANGQRD
                     ANAVEEKPKEAAPKDDVKVTNHGGGRFSIDGMMSETPSNQGEKSEAANAGERSLQQLR
                     EQFVTPRHVYDVPENNAVSQREPAAITLAEETPPQEQLSEQVKDLVQNVDALVERIHA
                     EEHKRQNAISAIETELKDSDDTDNLALWKNVFKTDERFTSAFSQNGGGTSINGTYIAM
                     KATREFGPFGIGWGVEVLEERFDKGAPIVRKKQVGEKIEWDLIQDGVGGYLCEMHHTM
                     KVRVWYILNGVRGESEAYGCTPYIYDTKYGPTSDGEAPKKSWTDAVKKALSPLGFSAD
                     IFMGLYDNPEYRQRNKAEFDIKNASENAEDATRLRKELDEKLINVANTLAAAVTANEV
                     NKVFGLIAREVDVHRKAAETKGDKEYSSYLGSRLRRITDIKTERLAALTAAQEQTA"
     gene            13120..13689
                     /locus_tag="QBH27_002601"
     CDS             13120..13689
                     /locus_tag="QBH27_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDQ7014899.1"
                     /translation="MSTAIAIAKEYASLIDLLETADELTPEMIADTLEGMEGELGDKL
                     DAMMVICRNLQGNANTCAEEMSRLATRKTSFEGKEKAIRKHMLTCLQAAGLDKLKTAK
                     NTFTDAQGAIRVIIDSKDKIPDEYEGVSLVDVETVITPNKRAIKEVIESAEAVAAEIL
                     ARGETPPAELLNPVPGAHLERGERSLRIR"
     gene            13689..13871
                     /locus_tag="QBH27_002602"
     CDS             13689..13871
                     /locus_tag="QBH27_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014900.1"
                     /translation="MLKLTLKRGDAVHLVLADGTNGIIEARSRCELGLHLPENIKVTR
                     EKSAFTPQELITPNQK"
     gene            14081..14344
                     /locus_tag="QBH27_002603"
     CDS             14081..14344
                     /locus_tag="QBH27_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDQ7014901.1"
                     /translation="MKSDHDIITREEMVELTGSPLKSKQCEALRRAGIFFMERADGHP
                     KTTWGHFMNPIKFRNLQEVTTRKDDEPDFGAIFNGRKEKEPSR"
     gene            14313..15329
                     /locus_tag="QBH27_002604"
     CDS             14313..15329
                     /locus_tag="QBH27_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ7014902.1"
                     /translation="MAGKRKNPADNWMPPRVYQGKAAYEFRNKDNKAIRLCALDAPRS
                     AVWLAYEKAVGDEKERNTFQALTEQFMTSPDFMDLAVETRKDYTKYSGKVLPVFGKID
                     PDKIKPEHIRRYMDQRGLSSRTQANREKSFMSRVFRWGYERGYVQRNPCQGVKQFKEK
                     ARERYITDEEYQAVYEVSPDVVRVAMEIAYLCVARQSDVLSLQKDQLFDSGIYIRQGK
                     TGVKQIKAWSPRLQKAIALARSLPLKTGISSLFVIHQTTGGKYTRDGFNSRWRDVKAA
                     AQEKYPHLQIDFTFHDLKAKGISDLEGSLEEKQAISGHKNPRQTAAYDRKVKVVPVVG
                     GQRK"
     gene            complement(15460..15546)
                     /locus_tag="QBH27_002605"
     tRNA            complement(15460..15546)
                     /locus_tag="QBH27_002605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15510..15512),aa:Leu,seq:taa)
     gene            complement(15559..15632)
                     /locus_tag="QBH27_002606"
     tRNA            complement(15559..15632)
                     /locus_tag="QBH27_002606"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15598..15600),aa:Cys,seq:gca)
     gene            complement(15686..15761)
                     /locus_tag="QBH27_002607"
     tRNA            complement(15686..15761)
                     /locus_tag="QBH27_002607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15726..15728),aa:Gly,seq:gcc)
     gene            complement(15913..16461)
                     /gene="pgsA"
                     /locus_tag="QBH27_002608"
     CDS             complement(15913..16461)
                     /gene="pgsA"
                     /locus_tag="QBH27_002608"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDQ7014903.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(16518..18350)
                     /gene="uvrC"
                     /locus_tag="QBH27_002609"
     CDS             complement(16518..18350)
                     /gene="uvrC"
                     /locus_tag="QBH27_002609"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDQ7014904.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(18347..19003)
                     /gene="uvrY"
                     /locus_tag="QBH27_002610"
     CDS             complement(18347..19003)
                     /gene="uvrY"
                     /locus_tag="QBH27_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDQ7014905.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            19299..19475
                     /locus_tag="QBH27_002611"
     CDS             19299..19475
                     /locus_tag="QBH27_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014906.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            19462..19686
                     /locus_tag="QBH27_002612"
     CDS             19462..19686
                     /locus_tag="QBH27_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDQ7014907.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(19754..20476)
                     /gene="sdiA"
                     /locus_tag="QBH27_002613"
     CDS             complement(19754..20476)
                     /gene="sdiA"
                     /locus_tag="QBH27_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDQ7014908.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(20706..21458)
                     /gene="yecC"
                     /locus_tag="QBH27_002614"
     CDS             complement(20706..21458)
                     /gene="yecC"
                     /locus_tag="QBH27_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDQ7014909.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(21455..22123)
                     /gene="tcyL"
                     /locus_tag="QBH27_002615"
     CDS             complement(21455..22123)
                     /gene="tcyL"
                     /locus_tag="QBH27_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDQ7014910.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(22138..>23046)
                     /gene="dcyD"
                     /locus_tag="QBH27_002616"
     CDS             complement(22138..>23046)
                     /gene="dcyD"
                     /locus_tag="QBH27_002616"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDQ7014911.1"
                     /translation="FSDYLGREIFIKRDDVTPMAMGGNKLRKLEFLAADALREGADTL
                     ITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEM
                     CDALTDPNAQLEELATRVEAQGFRPYVIPVGGSNALGALGYVESALEIAQQCEVAVNI
                     SSVVVASGSAGTHAGLAVGLEQLMPESELIGVTVSRSVADQLPKVVNLQQAIAKELEL
                     TASAEILLWDDYFAPGYGVPNDEGMEAVKLLARLEGILLDPVYTGKAMAGLIDGISQK
                     RFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         6227 a         5808 c         5181 g         5830 t
ORIGIN      
        1 ttcacttgta aatcttgaag ctatacatat ttactttcaa tgataaataa aagaactatc
       61 caaaaatcac tgagagcata ttttaactaa ccctccgcta ctattaacat aaatttcacc
      121 caaccataat taaattgata catatagcaa cttaccttga tacgaatcat ctgggttata
      181 tttttccata tttttttccg tcaaatcgaa tcatgccagg atcaagaaaa ttaattaaat
      241 cgattaacgc gtgctgtatt ctgattagcc tttctttata aattccgata tctcttgtca
      301 tatttatcac ttcattgttt aaatctaaaa aaaatggctc attgataaag caattttcct
      361 ttgtgaaact gccgtaacct atgcaatcaa atccagtatc tgttgatatc agcatctttt
      421 ctgcaatagc tctctgctct cctatgaaaa acataaaata ctgaccaaag acgtctgttt
      481 ggattaatga gttaatattg ttctgtaatc taataaactc cctcattttt ttattttttt
      541 ctaggctaag gtattgaatg tctatgcggg cagcttctgt ccatgcaaaa aactgggaaa
      601 aaagaaaaac agtattgttg ataacataat cttgttggcg cttattccca ttatgataaa
      661 actcttcaat gaaccccaat tctaatatat tataaatacg gctttgtaag tctgaagctg
      721 agcgaattaa cggttcaata tagtactgat gttttctcct tacatccagt gattcattga
      781 gcttgtttat tctattctga ttataagtag atatagcaat agagattata gctattgcac
      841 cactaataaa gcttgaaatt aatgccgtac ccatctaaaa gtcctttgat tcttgcttgg
      901 ttaacaaaat gacataattt tatttaatgc cataacaact taacaatata tcataatatg
      961 attattatgg tgtagcttgt cgcatctacg ctccattaca aacagatgta agatataacc
     1021 tatatgtttt agccagcaaa ttcgaaagca ttcaaagtag tctcatcgaa ctctcgaaac
     1081 cctatgtctt cgggtataaa atatttaaat ccatttgcat aatatcgact catgtttatt
     1141 atattctccc cattcaccat taagatttga cattattttc aacaatcggt tattgcaaga
     1201 ttgaaatata tttttacttg acacaatatt ccatgtagcc cagcgattcg acttaccccc
     1261 aactatacgg acaatctaaa ttaaatcgat gattttttga tacattctcc tctctccatt
     1321 actttatgtt tttgttcccc aaaaatacct tgctctctaa aaaataattg ttctttaagt
     1381 tcaaaatcga gatctacatg agcgttagat acaaagctaa tatgttgaaa tgtctttccc
     1441 ccccccataa cggcattgtt atctaaacta tgttacacaa atataatatt accctttcaa
     1501 cgtaatccaa gaataagatt aatgccatta tatatatcat aaatatgata ttttgtatat
     1561 ttttatcatt tagccttgat tgtactaggt gtactctcaa cgaagtaggg aaagtctgtt
     1621 cctcgctcac agctgccctt cagtaagcga aaaaatgcct actcggtgcc atatacagcc
     1681 attgttatta acagcaggga ataattcaaa actgaaatta aatatctacc ttttacagta
     1741 gaaatagttc ctccaggaca ctatccattg ccgggcacaa tcactcgggg tataacgata
     1801 taaaatatgt tcatgatagt gcaggttcgt atattaagtt cagcagcgaa aacatgcctt
     1861 acaggtttcc ggttttggga gcgtcgttac aggatcctgc gcataccgtt cccatctgtt
     1921 gaattaaagg tttcacagcc tttgccgata tcacctttta ccttacctgt gatgcgttca
     1981 gtgctcagcg ttgtttactg tagctctcgg gactgattac agaccgcgtc ttaaaaacca
     2041 atccttactc taaagggaat tcatgagatt aaaatactca gtactcttac tcgccattgc
     2101 ccttacaggc tgtgataaca aaaaagatga gatcggttgt tcttcggaaa taacccagtc
     2161 agctctcatg gatttattaa aaaaatctgc ttatgaagga ctctctgaac aggtcgataa
     2221 ataccctgat gtcactaatc agaccaaacg aagcacactg gacaagatca aactagccat
     2281 ctctgagatc tccacaacat caagtgacac gggcagcaca atgaaaactt gtgaagggac
     2341 cgtgacgatg actctatctg cgaacgagta cgtccagctt tcagatgctt acagaaagaa
     2401 ttttaatcgt aatctcgata agcaaatgga aagcctgttt ttggagaaca acgcaaacgc
     2461 cttttcaaaa cgtatcacct acaccgtgca ggcgaccgac gatcagaaaa acgtttttgt
     2521 aaaaacctct actgataatc cgatatcagt gggcgccgct ttactgacat cgctttccat
     2581 tatcaccccg atcgttgagc agcagaaaat ccagcaggct aaggatgccc aacagagtca
     2641 aattgaagcg gaacaacagg tgcaactcag ggcgcaagaa caggctaagt atgaggcgga
     2701 gaagcaaata gagagacagg cacaacttca gccgcaaaaa gctgacaacc ttgatcagtc
     2761 ccgaatggca tttgcgaatg ccgactctga tttgagtacc gtctggagca agttaacccc
     2821 ggcgaagaaa aaagatttgc tgccttctca gcgtcagtgg atcaaaacta aggatgctat
     2881 gtgcggtaaa gtttcaatgc agggaacaga tgctgaagtc aagaaaatgg tcgactgcca
     2941 gacgcaaatg accctttcaa gaatcgcttt cctcagaaac caataactga agtctccttc
     3001 aagctggcgc caggtgttaa cctggtgcca gcttagtcat caagtgcata tgtaactgtt
     3061 ttatatgggc ggtactttga cagaggtatt cggtctcgtg ctggtcgtgg tcaaatacct
     3121 ttttaagcgc aaatagagta tttccgcccc aaacaacctg tcaaaatgct gaattagcaa
     3181 cggatatagc cgactttttc cccattacca tcaatttatc cagtgtcata cttcgcagat
     3241 tgtcgctgtg gatcaccaga taccctgccc gctcagcgat ctctttgaaa gcgtcaaagg
     3301 tcatcagatg ttcgcctttg gcaacatggc gcatcccggt cacttttcca tttttgatag
     3361 tggtgataaa agaaaaatct ggaatggttg ggtgattttc atcgagtagc aggcagtgga
     3421 tgtattcaat cgcctctaac atttgcgtct gtgacagttg ttctatgtga gagacggcga
     3481 agcgttgatg aatcatggtg taggcatcgg gatacatcat gcctttcttg ccgaccagta
     3541 tattaacggc atcacgcaag ggagtacggt catcggtagt tgtactccgc ctctctggac
     3601 gctggctaaa ataacaatct tccagctttt cgaaaacttc ccaggcctgt tctgtttcca
     3661 gcattttggc atggcgagct gcgccgcgtt ctgtccagag aatgagggat cgggtatgtt
     3721 tagcaactga ctgactatct gtcagtctgt ttttgaagtc gcgtaattca ttaccttcca
     3781 gcttaaaaaa atgctttcct acgacaaatc tggcgatgtt gcgcttgaag ttttgctgga
     3841 taccatttac ttccgtgcca tacaaactgg caagcaattc agtagtgatg acgggaagtt
     3901 ggttatgatg tagcggagaa agggattccg cagatatttg gactgtcatc gcagtggcct
     3961 cacttagtgt ttttttcatc accaccagcg acgccaatca tctggtggtg agctgtgcag
     4021 ggttggcgta accggctaag tgaacccggc gcttccacag aagcccccac acagcccacc
     4081 ataatgcgaa tgtggccgtg cctaacacat aaaaaaaccg ctaacgcggc tatgcgtcac
     4141 ttagatatcc gggacgccaa tcccggcaac cgattttgcg gctgcgcgga aactatagcc
     4201 ccggactttt tctaagtcaa ttcaatcaac ttcaaacctc cggtgttcac attccataca
     4261 ctcaacggga acatcatcgc gatgcaagtc cacatatccg acccgcttat acaactcaat
     4321 aatgtccggt aacgtgacca gatgtttttc cggttccaac acctcagccc gggttacttt
     4381 gtaatctgaa atatagagca ctatccgccc gttccgtggt aactccagtt tatcgccaaa
     4441 catgtccttt ccctccggga aaccaatgat attgacgata ttacacccac aacgattcgt
     4501 tttacgtcac tccgcaatca ccatacatcc gtaccaatcc gtacattccc accacaaaaa
     4561 cgatatttga caggcactgg taatagattt ttaacgcgtg ctattgcctt gcgacgtatt
     4621 ttcaattcac tcatacagcc tccctgctct caaacggacg aatattggtt ggttctccac
     4681 acggaatagc ccaacccagc caggtaaata ttttcaggtc acacatcgcg tcataccgtt
     4741 cgccaggatt cacatccacc atgctgcagg caatttcata ttgttcacca ttatcagtca
     4801 gtgcgatctg gtcattaata aaccggggca caaatgcaag aaaaagttct ttcatgaaat
     4861 tctccgctca aaataaacaa actgattcac cgctccgatc ggcatttcga gtttttctgc
     4921 gatatcctgg cgattaatac ccgactgatg taattccctt gccagctcga tatcttcctg
     4981 gaggtatttc actgactggt gataattacc ccgcagatac atacagattt tcagctcgcg
     5041 cgcttttgtg cgtaccgctg caccggttct gccaataagc tgccctatac gttcaactgt
     5101 catgctgccc gcacattgtt tgatgattac gacttccgca cgcacccacc gtttgtattt
     5161 cattgttcca tcacccctct ccagtagttc agacgctctc tgaaaaattc ccggtacgta
     5221 tctggcgtca ttccgatttg ctcaatcacg cttcccctcg gaatacgttt ctcaaagagt
     5281 tggcggatca gcgccgccgc tcgcatgtcg tagtgctctt taagctggta ttcctggggc
     5341 catttggcgc ggttgtgtgg taagccgggc ggcaggtaat ctgattgccc ggccatatca
     5401 cgcccctttc tttgctatcg gagtgatgga actcagcagc aggcggcagc gaccaggcgc
     5461 gccaatgcgc tgcccggtta tcttgtcgta agtttcatgc ggtgaggcac accaggttgt
     5521 cgctgtttcg tggagtttca cttgccgctc accatttctg cgaatcgcga tcccggtgtg
     5581 cgttttgccc ttacgtttca ctgctggcgc tgcggctacg gatgcctgac gggcaggctt
     5641 agcaggggtt gaaaaattgc ctgctttagg tcttggcaca aaaaccgcgc gagtgcgcgc
     5701 acgtggcccg acgttcatat tccacaaaat gacgtcgaga tagtttttac cgtcgtctat
     5761 gtgtcctttg tccgacgggt actctaattt gcctggagtg gtggtcattg gtctttcctc
     5821 ggttatttca cgctggtcag gcgtggttaa aattcatttg tgcgagaatt atcgctacgg
     5881 gaataacgtt tatttttttc tgagcggtcg ttggtaaggc gataaacctc ctcatcgctg
     5941 atatcaataa ctgagccatt tttcatcagg gcataggctg ttcccgatgg tccttcgcgg
     6001 ttcagacgta gcaacatttc catgagattt gggtcggcat tttcgttata aacagcatca
     6061 cgatagaggc caatccagac atcgcaatct tgttcgatct gcccggtatc tttactgtcg
     6121 ctcgggttag gccgcttatc ggcacgttct tcaagctttc ggttgagttg cgtcagcagg
     6181 accacaacac aatccagctc ttttgccagg ttcttcaaac cggttgtgat atcgccatac
     6241 gcaatatccc tacgctctgc cgattcggtc ttcatcagcg tcaaatagtc aattgccacc
     6301 aggccaaccg agccacgcat ccgtttaact tttcgacatt cagcgacaat atgcgcaagg
     6361 gttacacctg ccgtgctgtc aatcatcatg ttggattcgg caatctcccc cgcctttgct
     6421 attgcgagac tcaattcaga ttcatcgtta gggccaagat aaaaaatctc cgaattgacc
     6481 tttgcttcct gcgcaaccat gcgttcaatg agacttctgt cagtcatttc caggctaaat
     6541 accagcgtcg gcaaacgatg attcagagca aaatgcaccg caattttatt aaacgcggct
     6601 gtcttgccca tttttggccg ggcaccaata acgaccaatg aacctcttaa cgcctgcttt
     6661 ggtgccatta agcgatcaag gctttcgatt ccgagcgtta gtccggctgc atgtgctgga
     6721 tcattgaaac ggcgctcaag gtcatctatc cagtcatcga cgacttcact tgccggacgc
     6781 aaaccgccct tatgcccggt acgggaatgt tcggccacct gcccaatcac ttgctgaacg
     6841 gcggtgatcc tgtcagtagc agtcatatct gcaggctgga tcataatctc cagacaagaa
     6901 ttcaactttt ccacggcata acgaagaatg gctttttcac gtacaatttt cgcgtagttg
     6961 accattgagg ccggaatcgc cgccttactc atttcagcca ggtatgcgaa tccccccacg
     7021 cgctcataca gccccttcga ttccatcagt tcggtcagtg ttatcaggtc agttggttta
     7081 tcagcttgaa ataatccttt gatctcccgg tagataacct gatgcgcatt gatataaaac
     7141 gtttccggtt tcagtaacgc aaataccgca ttggttctgt cgtggtcggt attcagcatc
     7201 agacagccca gaacagcctg ttctgcgtca acgctgtaca gtgcaatcgc gttatttatc
     7261 atttgcgcgt tcttccttga ccgaaacata gcagcgttcg gtaatcagat aatcaagatt
     7321 tttacgccgc cagaatcctc ccctgccatt tggcctgtcc tccagcatcc agcgacaatt
     7381 gccaccgata aattccagat aggcccgcca acgggactca ttgaaattga attttttcca
     7441 gaaagtgcga aggtgttttt ttctgccgtc agtgaggatt ttcactggtg gcatctctgg
     7501 caaaatctcg tgataggtat cgagtatcac ctgataattt attctctggt cggcaggttg
     7561 ttgggtgtcg gcgtcagccg attcaccatc agtaataatc tctgtagtat tctctgtaag
     7621 aatgtttgtt ggttttccac gtacaggcct ggtggttttc ccctcacctg tatgtggttt
     7681 ttccgcatcc ttgtatgcgg acgtaagtag ctggcacaac acatcattat cgattttgta
     7741 gtacagcctg gcaggcaagc cctgtttacg ttccagcaag acgccgagac cacgtagttt
     7801 tttgcgcgcg ccctcctgct cgtatcgtga aagccctgtt tcctcttccc actcatcctg
     7861 ggttttatat acccagccgt cgtcagttgt tctgtttgtc cagtacgtca actgcgacag
     7921 aaacaatgcg gcagtcacgc ccatttcaag acggataaaa cttctctgga aggctatggg
     7981 tctatcaagt aacgggagga tgttcatatg cccaaagcct ctgagagaac gcgacagcca
     8041 gcctcatagg cagcgccaga gagattttgc tcacgcagtt tcgctttttc ttcttcgtac
     8101 ttttcccata cagaacgtgc agctgcgacg cggccatcga atatggggcg gatctctgca
     8161 acatgtgccg ggcgtccatt caggtgccag ccgttacgcc aggtaatgcg gtcaatatgt
     8221 cttaacatcg gtctttcctc ggtataagtt aaacgctggt caggcgctca tgcatggtgg
     8281 tcttgcgcag tgcgtatcac tgctcctcgc gtcgctacca gcgccgctat tgcttcgtca
     8341 atttcctgaa tagttaactc tggcgcgaag tggagatgta cagcgttaat cgcttccacc
     8401 ccttcttttg ccgccagcgt tgccagcaaa acgggatcgc ccggcgactc cagacgtgcc
     8461 cgccgttctg cgggtagcac ggctttcatc acactggcca gcacctgagt ttttcggcgc
     8521 gccgccgttg tctcgccacg taaccagcga aagattttct gccgattgtt gttgatggct
     8581 ctccagtcca cgttgccgtc cgcgtcctca aattcatgta gtctcaactc atcattgcgt
     8641 ccctgactga accaggcccg gcagatttca atcgtgacga gttcctgccc tgcccttgcc
     8701 gcccaggtca aaatctcttt ttgtaattcc tcttggtttt ccatagcgtc tcctgtcgct
     8761 aaaaattgat tacgcttaat cagatttggg gctcaccaac agttaagctg cttccgtttt
     8821 aggcaagctg tcatctgggt ttgggtaaag atccggtcta agatcatgag gggtcacgcg
     8881 ccattcaaga gcttcagatg tgcgcaaaac ctcttcaccg ggaacccgtc ctttgaacca
     8941 gaggcttacc gtttgtggtt ttttccctaa tcgccgccct aactcggatt gactcattac
     9001 ggaaagaatt ttgtcttgca gttgtttatc catatggact ccttgtgtcc gcgtcatcat
     9061 tacaaacaat aactgtaatt acaaattata tttgcaatgc tccctacaat taaaccttgt
     9121 atccttacgg tatgaacaca aaaacgaaaa acatggcttt tgctagccga ctacaacgga
     9181 ttctaaaaga cttaggctgg tctcaatctg agttagctcg ccatattggg gttacggctc
     9241 aatcagttca ggcatggtgt aatggggtaa ctccaagaaa agataaatta gacaagctag
     9301 caaaagtgac cggataccct gttcattttt tcttcatgaa tgaaggggaa tatttggatg
     9361 aatccacact acacccgggt aattacaatc aagaactaac cccgcaagaa caggctctac
     9421 tgcaactatt cagaggactg cctgagagcg aaaaaaataa attaatcaat gagctaaaag
     9481 aaaaaagaga gcacttcgat ctactgctaa aagaactact tgaagccaaa aaccagacaa
     9541 aatagttcag ttcaaatttc caggccagcc gctgctggca tgcaactttc ccctaacaca
     9601 aaaacacctt tacacagtga atattttttt gcccctcact acaaattatt ttttaaaaac
     9661 acattgacca ttacaaatac aaattgtaaa gtcactttca tcaacaacgc ttacccagcg
     9721 gcagttgttc agaaacacgt tctgacagcc ggaaagacgg caccaaattt tgcgcgtcgg
     9781 cgccaacacg gtgacagagg gaaagacttc accggcatat ggcacatgtg tcgaagcggt
     9841 ctggatggaa gcggagcctt aacgcgttgt ctccatagca ggtagccgga atgtgcaagc
     9901 cacagccagg tatgagcgat tgattcacca tcaaggcgat acggtgtgac caccagggaa
     9961 gagtcctggc tacaacacga gagcgcactt catcgactca actttgagct ttgtcgttaa
    10021 attttgaaat ggcggagtgc gctcccggtt gtggtgaaca ggtgtttaac gggaactccc
    10081 tgcccgttac ccggttcgat tccgggcgcc catcatcaat ttgctgtgtt tagtctttgc
    10141 ccagtccgca cgatgggcca ttttttcaca cagccaggtt ttatcgctgt gcctgagtcc
    10201 ccaacaggag aggccaaacc cgcagcgtga caccagggaa agaccggagg aagtaccacg
    10261 cctgaccagc gttgaccatg agcctgacca gctcaaaaca ggaaagacca gcccgggcct
    10321 gaccagccct gtacggtcgt aatggaaaca taacgacgcc ggaaacgtaa ccggcaccct
    10381 ttagatagca aaagacccgc acaaggcggg ccagttaccc cgaacggcga ccaaaccatt
    10441 cggatttatc acaagtgacc aaacttgtga tgaggaaaga ccaacgacac tgacgctatg
    10501 gaagctgatc aatattcgct gatcggctct gagtatacat taccaaggag tcgctatgga
    10561 agcgcgcacc atcccagtaa cactctttat tcattatgca acttcaactt tcagccacga
    10621 aaagctgctt gttgcgactg ttgatatgtc aaaaaatttt ccagacaggt acatccttct
    10681 ggaaagccgc gaaattgaaa ttaccgttaa ccagcccgag ccaatcgaca tcatcggttt
    10741 acaggtcgag caactgcgtg aacaaaaaca gaaaacagtc gccgacgccc aacagcgtat
    10801 tgctgctatc gatgacaaaa tccagcagtt actttgcatc gaatacacgc cagataccga
    10861 tgagctcccc tactaaaaac cactgacctg taatgaggaa agaccaatga ccatttttaa
    10921 cggcttgtta gaagcgaaaa aaggcgcgct caaaaacggc gcgatcccgg cgctggccat
    10981 cgccatcgcc gcccctaaca aaaaagttgc cgagaacatc atcatcggca aattgtggga
    11041 agcctaccct gaccacggcg acaactattt caaacctaaa atctgggaag atgccccggg
    11101 ccaaccgcgc ccaggcgtcg gtgagtttga tgagacgttt gccacagaac acagttttga
    11161 tggcgaaaaa tgggtagtta acactcctgt tgattcagat tgcagttcag cagatatctc
    11221 acaggttagc gacctgatga aactgccggc tcgtgaacga ttcgccgccg ttctgctgtt
    11281 cagccacgac gccaacgaag tcgacagtga attgcttgtg cagacgcgtg aatacctgga
    11341 gatgctcgat aacagtgata ctgacagtga ggatgaggtt gacgcgttta accgcatcgt
    11401 tcttgatgcc atggtggcgt gtaagcccat cgagtacatg catattgctg gattgaataa
    11461 tctggtacat gcaattttgg caagttgcga tacccaggaa caaaacccga ccagttggac
    11521 tatctccaaa tttataaaaa aatgggtaga gaatcccggt aaacgagatg aaatgctgcc
    11581 ggaggtaaaa ccagaaacag catccgcacg tccttacaaa cagactcacg ccactctgga
    11641 tcgtgaaatt gcctgcgccc tgttacctgt tgccccggaa aaaatcaccc ccagcatcct
    11701 gaaagcggca gacgaaataa tcagtcagga cagggaagat tttaaacgct ggtcaatggc
    11761 cttgcgcaca acggatcaga tccttgccta tgaccgcgca tcagtattcg gtgttattca
    11821 gagcgccccc gcaaaagaca cgtaccactt cccacaatcc ctgcgcagtc acatcgacaa
    11881 ctggctgcaa gctaacgggc agcgtgatgc aaatgctgtt gaagagaagc caaaggaagc
    11941 agcacccaag gatgatgtaa aagtcaccaa ccacggcggc ggacgattct caattgatgg
    12001 gatgatgtca gaaacaccct caaatcaggg cgaaaaaagc gaagctgcaa atgctggaga
    12061 acgtagtttg cagcagttgc gtgagcagtt tgtcacgcct cgccatgtgt atgacgtacc
    12121 tgaaaataac gccgtatcac agagggaacc tgcagctatt accctggcag aagagactcc
    12181 tccacaagaa caacttagcg agcaggtaaa ggatctggtg cagaacgttg acgcgctggt
    12241 tgagcggatc catgccgaag agcacaagcg gcagaatgca atctcagcta tcgaaacgga
    12301 gttaaaggac tcagatgaca cggataacct ggcattgtgg aagaacgtat tcaaaaccga
    12361 tgagcgcttc accagtgctt tctctcagaa tggcggcggc acatccatca acggcactta
    12421 tatagcgatg aaagcgactc gcgaatttgg gccatttggt attggctggg gggtagaggt
    12481 gctggaggag cgctttgata aaggcgcacc aatagttcgc aaaaaacagg ttggtgaaaa
    12541 aatagaatgg gatctgattc aagacggtgt tggcgggtac ctatgtgaaa tgcaccacac
    12601 catgaaagtg agagtctggt acatattgaa tggggtacgc ggtgagtccg aggcttacgg
    12661 atgcacacct tatatctacg acactaaata cggaccaaca agcgacggcg aagcaccgaa
    12721 gaaatcctgg acggatgctg ttaagaaagc gctatcccct ctcggattca gcgccgatat
    12781 tttcatgggc ctgtacgaca atccggaata ccgccagagg aataaagctg agtttgatat
    12841 caagaatgcc agcgagaatg cggaagatgc tacccgtctg cgtaaggaac tggatgagaa
    12901 gcttatcaac gttgccaata cgctggccgc cgcggtaacc gccaacgaag taaacaaggt
    12961 atttggcctc atcgcccgcg aagtggatgt acacaggaaa gcagcggaaa ccaaaggcga
    13021 taaagaatat tcatcgtatc tgggatcacg tctgcgccgt atcaccgaca ttaaaactga
    13081 acgcctcgcc gcccttaccg ctgcacagga gcaaacagca tgagcactgc tatcgctatt
    13141 gcaaaagaat atgccagtct gattgacctg ttagaaaccg ccgatgaact gacgccagag
    13201 atgatcgccg acacgcttga aggcatggaa ggagaactgg gagacaaact ggacgccatg
    13261 atggtcatct gccgtaattt acagggcaat gccaatacct gcgctgaaga aatgtctcgc
    13321 ctggcaaccc gtaagacgtc ctttgaaggc aaggaaaaag caattcgaaa gcatatgctc
    13381 acctgcctgc aagctgctgg cctggataaa ctgaaaaccg caaaaaacac atttactgat
    13441 gcacaggggg ctatccgggt aattatcgat agcaaagata agatcccgga tgagtatgag
    13501 ggcgtgtcac ttgttgatgt agaaacggtt attacaccaa acaagcgagc cattaaagaa
    13561 gtgattgaat ctgcggaggc ggttgccgcc gaaatactgg cgcgtggaga aacaccacca
    13621 gccgagttat taaacccggt accaggcgca catctggaac gcggcgaacg gtcactgagg
    13681 atacgctaat gcttaaactc acattaaagc gtggtgacgc ggttcacctg gtacttgccg
    13741 atggtaccaa tggcattatt gaagcacgga gccgctgcga acttgggcta cacctgccag
    13801 aaaacattaa ggtcactcgg gagaaatcgg cattcacccc acaagaactg attacgccta
    13861 atcagaaata aaaactcacc atcgctagca ttgcgattca ccagacaccg gaggatcaca
    13921 atgctgcgat ggcaaccggg agcaactctg ctctccgcat ttgatatcaa aattggtcga
    13981 ctgtcggcca gcgtcaggaa acagactttg accgagtctg atattgcccg tgcctgccag
    14041 aaggcagatg acgcaataag ctacataatg aggaaagacc atgaaaagcg atcacgacat
    14101 aatcaccaga gaagaaatgg tcgagctgac gggaagccca cttaaatcaa agcaatgtga
    14161 ggctcttcgc cgggctggaa ttttcttcat ggaaagggct gacggacacc caaaaacaac
    14221 ctggggccat ttcatgaacc caataaaatt tcgcaattta caggaggtga cgacgcgaaa
    14281 agatgatgaa cctgattttg gagcaatatt taatggccgg aaagagaaag aacccagcag
    14341 ataactggat gccgcctcgt gtttaccagg gcaaagcggc ctacgaattc aggaataaag
    14401 ataacaaagc gatacgcctg tgcgcattgg atgcaccacg atcagccgta tggctggcat
    14461 atgaaaaagc ggtcggtgac gaaaaagaaa gaaatacttt tcaggcgctc acggaacaat
    14521 tcatgacctc ccctgatttt atggatttgg cagtcgaaac ccggaaagac tacacaaaat
    14581 attccggaaa agttctgcct gtcttcggga agatcgaccc ggataaaatc aaacctgaac
    14641 atattcggcg ctatatggat caacgcggtt tatcaagccg aactcaggca aacagggaaa
    14701 aaagctttat gtcccgggta ttccgttggg gttatgaacg aggttatgtt cagaggaatc
    14761 cttgtcaggg agttaaacag tttaaagaga aagctcgcga acgttatatt acggatgaag
    14821 aatatcaggc cgtttatgaa gtgtctcctg atgttgttcg cgtagcaatg gagattgcct
    14881 acttatgcgt ggccagacag agtgatgtac tttcattgca gaaagaccag ctgttcgatt
    14941 ccgggatcta cattcgtcag ggaaaaaccg gcgttaagca aatcaaagcc tggtcgcctc
    15001 gtctgcagaa agcgatagct ctggctcgtt ctctgccatt aaaaacggga atcagtagcc
    15061 tgtttgtgat tcatcaaact accggtggca agtatacccg tgatggtttt aactctcgtt
    15121 ggcgtgatgt caaagcggca gcacaggaaa aatatcctca tctgcaaata gacttcacat
    15181 ttcatgatct gaaagcaaaa ggtatctctg atctggaagg cagcctggaa gagaagcaag
    15241 caatttccgg gcataagaac ccgcgacaga cagcagcata tgaccggaaa gttaaagtag
    15301 tgcccgtagt tggtggccag agaaaatgaa tgatgatgcg tccagagaaa aatcatcttc
    15361 ggacgcatct tcggaaatga gaaagaagat acaaaaaaac cacccgaagg tggtttcacg
    15421 acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg
    15481 aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat
    15541 ccgggctcgg gaaaaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc
    15601 aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat
    15661 ccgctgacgc cgatgccttt taaactggag cgggaaacga gactcgaact cgcgaccccg
    15721 accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt
    15781 aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta
    15841 cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt
    15901 gcgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat
    15961 tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt actcaaccca
    16021 aatattcgga cgccacagca gccatgccaa cgccaccatc tgggcagtgg ttttcacttt
    16081 tccaatccag gagacagcca cgctactgcg tttacccaac tccgccatcc attcgcgtag
    16141 cgcagaaata ataatttcac gggcgatcat cgttgctgcc ggtaaggtca cccaccagct
    16201 gtggtaatgc tcggttacca gcaccatggc gatagccacg agaactttat ctgcgacagg
    16261 gtcaaggaaa gcaccaaacc gggtactctg attccagcgg cgtgccagaa aaccatcgaa
    16321 ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac
    16381 aggcaaataa aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa
    16441 cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct
    16501 atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc
    16561 gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg
    16621 cccatatatt tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg
    16681 gaactggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt
    16741 gattcatcgc gaatatgctg gataacatgc agcgcgggag aatctggcgg caaactaaat
    16801 ccctcacctt ccggctcaaa gaacagcgtt tccagcccag ccttacgatc tgctcctttg
    16861 gcaacgccaa gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaaaaca
    16921 tttttcgcct gcgcaagctg gcctttgccg ccgtcaataa ggatcacatc cgggatctta
    16981 ctgtcgtcga tagctttacc ataacgccga cgcagcacct gattcatcgc cgcgtaatca
    17041 tcgcccggcg tgatgccagt aatgttatag cgccgatact ccgcacgcag cgggccgttg
    17101 gcatcaaaca ccacacagga agcgacggtt tgttcaccca tggtatggct gatgtcaaag
    17161 cactccatcc gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagccgctgg
    17221 tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtacgcgcg
    17281 agtttcagat aacgcgctct atcgccacga ggtttggttt gaacattaat cttgcgtccc
    17341 gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg
    17401 atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt
    17461 tccaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg
    17521 agcactttgc cctgacgaat gaacaatacg tggacacaag ccatacccgc atcgaacgcc
    17581 actccaataa cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacacgt
    17641 cgcaccgcct gaatttggtc gcgaatccgc gccgcttctt caaactccag attctggctg
    17701 gcagtttcca tacgactaat gagttgcgta agcacctgat catctttgcc agacaaaaac
    17761 aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac
    17821 ggccccagac agcgccctat ctgatattgc agacacggac gcgagcggtt gcgataaaca
    17881 ctgttttcgc actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg
    17941 gcatagccat tcgggaacgg gccgaaatat tcacctttgg catgcttcgc tccacgatgc
    18001 atcgccagac gcgggtgggt atcgccactc aggaagataa aaggatatga tttatcatcg
    18061 cgtagcaaaa cgttgtaacg cggctgataa agtttgatgt agttgtgttc cagtaacagc
    18121 gcttcggttt ctgtatgagt aaccgttaca tcaatttgct ggatctgggc gaccagcgct
    18181 tcggttttgc gcgaagcgag gttgctacgg aaatagctgg aaagccgttt tttcaggtct
    18241 ttcgctttgc cgacatagat aaccgtacca ccagcatcat acatgcgata aacgcctggc
    18301 tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata
    18361 atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat
    18421 gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat
    18481 tgagctgttc tgagatctca ttgaccttct ggcccttggt gatcatcagc ataatctgca
    18541 attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta
    18601 acgccatttg ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa
    18661 tcgcactcac gacttcctgc ggagccgcgc ctttgctgag gtagcccgca gctccggcct
    18721 gcatgacttt cgctggtaaa gggttttctg tatggacggt aagcatgatg attttgacat
    18781 cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca
    18841 tgtccattag caccacgtca acggcatttg cccggcacca tttaacggcg tcttcaccgc
    18901 acgatgcctc accgacgact tttataccct ttatatcttc cagaatgcgt cgtatccctg
    18961 cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa
    19021 tagggataac gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg
    19081 aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac
    19141 agttatggtc acgcccgtca ccaacggctt ccgggatcgt ttcacagtaa gcccggcgat
    19201 gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta
    19261 acattaatta acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc
    19321 attatattaa cgtattttat agtactgttg atatcacgct caaaaaacaa ccactgctat
    19381 ttttagcaaa gcttatggta tactccgccg ccttaacatt tttcaccgca aattttcatt
    19441 gcaacatgac gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa
    19501 gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg
    19561 ggacaagctg acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa
    19621 gacgaaaccc aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt
    19681 cagtaatata aaaccggctg agagcgtgcc tttcagccgg tttttgcatc tggcacgcag
    19741 aacagaaaag agatcaaatt aagccagtag cggccgcgta acaggcaacc tgcgtcttat
    19801 tcggtgcatt gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga
    19861 ttgacaaaat catcgctatc tctgctgatg tcttaccttc cgccgtccat ttcagaattt
    19921 ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca
    19981 tcagagccat cagactttcg cgcaccagta gctgcatttt taattgcagt tcatcactaa
    20041 gaatgggtat ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccaac gcccgatttg
    20101 gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgcgcgca gcttcccata
    20161 acggctgtgc ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt
    20221 cagggttcag caccggatca attgcgagaa aattttttgc ctgataataa ctaacccacg
    20281 actcagggta attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa
    20341 cacataacga atagtaatcg tactccagct gctgagcctg aagctctatt tcatggtaga
    20401 cctcttctgc ggcctccatc ctctgaaaac gcaacagcat cgtgcgaagc cagctgaaaa
    20461 aatccgtatc ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat
    20521 aattcaaact atcacataaa taagatttat atataattta tattattcag gcaatgaatt
    20581 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca
    20641 gttttatcac gtttgaaatg gcgtcccgcc ccgactggtg aatcgtcggg gctgattttt
    20701 tcttattatt gcagcagaaa cttctccagg aactggcggg tgcgaggctg ctgaggatca
    20761 gcaaataacg cttttgctac cccctgctcg actatccgcc cctggtccat aaaaatcgcc
    20821 cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt
    20881 tcctgcgcca gctgacgaat ggtgttcagg acttcgccca ccagctctgg atccagcgcc
    20941 gacgttggct cgtcaaacaa aatcacttca ggacgcattg ccagagcacg cgcaatcgca
    21001 acgcgctgct gttgaccgcc agacaaacga cgtggataac tggtttcttt accggccagc
    21061 ccaactttcg ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
    21121 acgatcaccg gcccttcaat aatgttttcc agcaccgtac gatgcggaaa cagattaaag
    21181 ttctggaaga caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt
    21241 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tggttcccgc ttccggttgt
    21301 tccagcagat ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt
    21361 gccactactt cgcctggctt cacctcaagg tcgataccat gcagcaccgt ctgaccgtgg
    21421 aattttttca ccaggttctt aacttcaatg gcactcattt gggttctctc tcctggcgat
    21481 taagttgatt ctcaaaatgg ttctgcaacg tcgataacac agtcgccatg atccagtaga
    21541 tgagcgaagc cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg
    21601 cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc
    21661 tgataaagct gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc
    21721 gacgcatggt ctgccatggc gtcataccaa tactggctgc agcttcccac tgacctttat
    21781 caatagaaga aatggcggcg cgcagcgttt cagcagcata ggcggcagtg ttcagcgaca
    21841 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgtggc agtccgtaat
    21901 agatcataaa cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg
    21961 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca
    22021 gtaagccaaa aaacatgcct ccgatactga gttgcagcgt atacccagcc cctttcagca
    22081 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
    22141 aacgtgggga tgataggcga acagcgcagg cgcaccgccg gtatgaataa acaaaatcgg
    22201 cccttcatct ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc
    22261 ggtatacaca ggatcaagca gaatgccttc cagcctcgcc agcaatttca ctgcttccat
    22321 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc
    22381 cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tggaggttaa ccactttcgg
    22441 caattgatcg gcaactgaac gcgacacagt cacgccaatc agttcgcttt caggcatcag
    22501 ctgttccagc ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgaccaccac
    22561 cgacgaaata ttaaccgcca cttcacactg ttgcgcgatt tccagcgcac tctccacata
    22621 acccagcgcg cccaaagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
    22681 tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca
    22741 catttcaatc tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt
    22801 ttccgcggtt gtaccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc
    22861 agcgactgcg gcagtctggc gcacatggtt agactggatc gccccggcag taatcagcgt
    22921 atcggcacct tcacgcagag catctgccgc gagaaattcc agcttacgta atttattgcc
    22981 gcccattgcc atgggagtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc
    23041 agaaaa
//
LOCUS       DAOFSV010000064        21951 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.064, whole genome shotgun sequence.
ACCESSION   DAOFSV010000064 DAOFSV010000000
VERSION     DAOFSV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.064"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(72..1250)
                     /gene="purT"
                     /locus_tag="QBH27_002617"
     CDS             complement(72..1250)
                     /gene="purT"
                     /locus_tag="QBH27_002617"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDQ7014912.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            1384..1674
                     /gene="yebG"
                     /locus_tag="QBH27_002618"
     CDS             1384..1674
                     /gene="yebG"
                     /locus_tag="QBH27_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDQ7014913.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            1741..2097
                     /locus_tag="QBH27_002619"
     CDS             1741..2097
                     /locus_tag="QBH27_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDQ7014914.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            2424..3083
                     /locus_tag="QBH27_002620"
     CDS             2424..3083
                     /locus_tag="QBH27_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDQ7014915.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDLQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            3292..5352
                     /gene="ptrB"
                     /locus_tag="QBH27_002621"
     CDS             3292..5352
                     /gene="ptrB"
                     /locus_tag="QBH27_002621"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDQ7014916.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVDPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEIADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(5349..6011)
                     /gene="exoX"
                     /locus_tag="QBH27_002622"
     CDS             complement(5349..6011)
                     /gene="exoX"
                     /locus_tag="QBH27_002622"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDQ7014917.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(6035..6691)
                     /locus_tag="QBH27_002623"
     CDS             complement(6035..6691)
                     /locus_tag="QBH27_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDQ7014918.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(6793..7023)
                     /gene="holE"
                     /locus_tag="QBH27_002624"
     CDS             complement(6793..7023)
                     /gene="holE"
                     /locus_tag="QBH27_002624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDQ7014919.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            7162..7536
                     /gene="yobA"
                     /locus_tag="QBH27_002625"
     CDS             7162..7536
                     /gene="yobA"
                     /locus_tag="QBH27_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDQ7014920.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            7540..8412
                     /gene="copD"
                     /locus_tag="QBH27_002626"
     CDS             7540..8412
                     /gene="copD"
                     /locus_tag="QBH27_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDQ7014921.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTHWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            8425..8766
                     /locus_tag="QBH27_002627"
     CDS             8425..8766
                     /locus_tag="QBH27_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDQ7014922.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            9161..9817
                     /gene="pphA"
                     /locus_tag="QBH27_002628"
     CDS             9161..9817
                     /gene="pphA"
                     /locus_tag="QBH27_002628"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDQ7014923.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(9818..10009)
                     /locus_tag="QBH27_002629"
     CDS             complement(9818..10009)
                     /locus_tag="QBH27_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDQ7014924.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(10114..10350)
                     /locus_tag="QBH27_002630"
     CDS             complement(10114..10350)
                     /locus_tag="QBH27_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDQ7014925.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(10467..11906)
                     /gene="rsmF"
                     /locus_tag="QBH27_002631"
     CDS             complement(10467..11906)
                     /gene="rsmF"
                     /locus_tag="QBH27_002631"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDQ7014926.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(11986..14619)
                     /gene="yebT"
                     /locus_tag="QBH27_002632"
     CDS             complement(11986..14619)
                     /gene="yebT"
                     /locus_tag="QBH27_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDQ7014927.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(14588..15871)
                     /gene="yebS"
                     /locus_tag="QBH27_002633"
     CDS             complement(14588..15871)
                     /gene="yebS"
                     /locus_tag="QBH27_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDQ7014928.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLLLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            16001..16498
                     /gene="msrC"
                     /locus_tag="QBH27_002634"
     CDS             16001..16498
                     /gene="msrC"
                     /locus_tag="QBH27_002634"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDQ7014929.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            16595..17293
                     /gene="proQ"
                     /locus_tag="QBH27_002635"
     CDS             16595..17293
                     /gene="proQ"
                     /locus_tag="QBH27_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDQ7014930.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKSGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            17313..19361
                     /gene="prc"
                     /locus_tag="QBH27_002636"
     CDS             17313..19361
                     /gene="prc"
                     /locus_tag="QBH27_002636"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDQ7014931.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            19553..20434
                     /gene="htpX"
                     /locus_tag="QBH27_002637"
     CDS             19553..20434
                     /gene="htpX"
                     /locus_tag="QBH27_002637"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDQ7014932.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(20480..21853)
                     /locus_tag="QBH27_002638"
     CDS             complement(20480..21853)
                     /locus_tag="QBH27_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7014933.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT         5668 a         5466 c         5629 g         5188 t
ORIGIN      
        1 aggcgctaag ctgtaaaagt caaaatgccc gatcgaggat cgggcatttt tgtagcgttt
       61 ttttgccggg tttaaccctg tacttttacc tgtccggcgg cgtgcttcgc gcgttcaatg
      121 gcgtcaacaa cactctctgc agtagccagt gctaccccca gacgacggct gccatcaatt
      181 tccggcttac caaataaacg aatctgcaaa tctgcgccta cggcattctg cacattatca
      241 aacgtgacat tctgactggt cagttgcggc aggataacgg cagaagcagc aggaccatac
      301 tggcggatcc cgccaaccgg aagtccgagg aaggcacgta catgcagggc aaactccgag
      361 agatcctgag aaattagcgt caccatcccg gtgtcatgcg ggcgagggga gacttcactg
      421 aaaatcacct catcaccaca gacaaacagc tcgacaccaa acagcccata accgccaagt
      481 gccagcacca ctttacgggc aatctcctgc gcacgttcaa gggcaagcgg gctcatttgc
      541 tgtggttgcc aggattcacg atagtcgcca tcttcctggc gatgacctac cggtgcacag
      601 aaatggacgc catccaccgc actgacggtt agcagggtaa tttcgaagtc aaacttaacg
      661 acgccttcaa caattacgcg gcccgctccg gcgcgaccac cttgctgagc gtactcccat
      721 gcatgagcaa gttgctctgc agaacgaata aacgtctgcc ctttaccgga agaactcatc
      781 accggtttaa caatacaggg gtagccaatg gcggcaaccg cttcgcggaa aaggttttcg
      841 ctatcggcaa aacgataagt ggaagtgggc agctgcagct cttctgccgc caggcgacgg
      901 ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc aggggacaac attcagtcct
      961 tcctcttcaa gttggatcag catatcggtg gcaatagctt cgatctccgg cacgatataa
     1021 tgtggttttt ccagttcaac cacacggcgt aatgcatcac catcaagcat attaatgaca
     1081 tgggagcgat gcgcgacatg catggctggt gcgtcggcat agcgatcgac ggcaatcacc
     1141 tctacgccga gacgctgaca ctcgattgcc acttctttac ccagttcacc ggagcctaat
     1201 aacatcacgc gagttgctgc cggacgcagc gcagtgccta ataacgtcat aacgaatatc
     1261 ccctgattaa ttccgcgcgc agtataaacg aaaacgtttg cgtgtgtctt tattgctgat
     1321 gttgatttcc caactgaaaa gaaatatact gtataaaacc acagttatta tgagaggctg
     1381 caaatggcgg ttgaagttaa atacgtagtc attcgtgagg gcgaagagaa aatgtcgttt
     1441 accagcaaaa aggaagccga tgcttatgac aagatgcttg atacggccga tctgcttgac
     1501 acctggctga caaattctcc agtgcaaatg gaagacgagc aacgtgaagc cctttcgcta
     1561 tggctggcag aacaaaaaga tgtgctgagc accattctga aaaccggcaa attaccgtcc
     1621 ccacaggtcg tcggtgctga atccgaagag gaagatgcct cacacgcggc ctgaagcaac
     1681 ttgcgctcct gacaatttgt accatgcttt ctcctgaatc attgtgctat ggagaaaaac
     1741 atgaaaaaaa gaggggcgtt tttagggctg ttgttggttt ctgcctgcgc atcagttttc
     1801 gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg tctggatgct
     1861 gccggaattg ccgcgagcgt aaaacgtgat taccaacaaa atcgcgtggc gcgctgggct
     1921 gatgatcaaa aaattgtcgg tcaggccgat cccgtggcat gggtcagttt gcaggacatt
     1981 caggggaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag tgccgatatt
     2041 cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg aatatcagcg gcgttaatgg
     2101 cttctgataa ttcgattgac cgttggcgga tgcggcgcgg gtgccttgtc cgccctacaa
     2161 cgcaacacaa tcgtaggcct gagaagacgc agtagcgtcg aaccgggcaa tcagcatcaa
     2221 acagttccac tcaaaatgtc aaactacttt cacctaaaat acccttatca ccttatatac
     2281 agtgaacacc ttacctggcg cgaatttctt tgaataacgg cctaccgccc cgcttatttg
     2341 cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct acaggcacta
     2401 aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa tccctgcttg
     2461 ggcaaagccg ttcttctacc tcctcgtctg cggatcaggg attgggcaaa cttttagtgc
     2521 ctggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca cgtaaacttc
     2581 ttacgaaata tggcacaaac gcgttactgg ttggcggcgg agcggtagcg ggtacggtgc
     2641 tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg cagtttggcg
     2701 cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg gtctttgccg
     2761 ctaaaagtga tggtcatatt gatgccaaag aacgtgcggc aatcgacctg caattgcgtg
     2821 aagccggtgt ggaagagaag gggcgtgtac tcattgagca ggcaatcgaa caaccgctgg
     2881 atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa atctatttcc
     2941 tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg aatgcactgg
     3001 gtgatgcgct gaaaatcccc caggacgttc gtgaaggcat tgaacgcgat ctcgaacagc
     3061 aaaaacgtac tttggcggag taattccgct ctctggatag cctgaagttg actatttcgg
     3121 gttatccaga accgcacagc catttgaaca ggctgatttt ctctgtctgc ataacagtcg
     3181 atatccgcgc cattacctgt catcatctaa gcaatgacta ccctgtttcg cttgcatccc
     3241 cggtgagttt tgccaccctt ataagatgtt tcaaccagaa agaacaataa catgctacca
     3301 aaagccgccc gcattcccca cgccatgacg cttcatggcg atacgcgcat cgataattac
     3361 tactggctgc gggacgatac gcgttctcag ccggaagtcc tggactatct gcaacaagaa
     3421 aatagttacg gtcatcgggt gatggcctcg caacaagcct tgcaggatcg catcttaaag
     3481 gaaatcatcg accgcattcc gcaaagagaa gtttctgcgc cctacataaa aaatggctac
     3541 cgctatcggc atatttatga accaggctgt gaatatgcta tctaccagcg tcaatcggcg
     3601 ttcagcgaag agtgggatga gtgggaaaca ttgctcgatg ccaacaagcg cgcggctcat
     3661 agtgagtttt attcgatggg cggaatggcg attacgcccg ataacaccat tatggcactg
     3721 gcagaagatt ttctttcccg acgccagtac ggcattcgtt ttcgtaatct ggaaacaggt
     3781 aactggtatc cggaactgct ggataacgtt gaccccagct ttgtctgggc aaatgattcc
     3841 tggactttct actatgttcg caagcatccg gtgacgctgc ttccttatca ggtctggcgt
     3901 cacgccatcg gtacgccagc atcgcaggat aaactgatct acgaagaaaa agacgatacc
     3961 tattacgtca gcctgcataa aacgacctcg aagcactatg tagtcattca tctggccagc
     4021 gccaccacca gtgaagttcg cctgctggac gcggaaatag ccgatgccga gccgtttgtt
     4081 tttctgccgc gccgcaaaga tcacgaatac agccttgatc actaccagca tcggttttat
     4141 ctgcgttcca accgccacgg taaaaacttt ggcttatacc gtacccgtat gcgtgatgag
     4201 caacagtggg aagagttaat tccgccacgt gataacatta tgctggaagg gtttacgctg
     4261 tttaccgact ggctggtggt tgaagagcgt cagcgcgggt taaccagttt gcggcaaatt
     4321 aaccgcaaga cccgggaagt cattggcatt gcctttgatg atccggccta tgtgacctgg
     4381 attgcctaca atccagaacc tgaaaccgcg cgattgcgtt atggttattc gtccatgacc
     4441 acaccagata ctttgtttga actggatatg gataccggtg agcgtcgtgt attaaaacaa
     4501 acggaagttc ctggttttga tgcggcgaat taccgcagtg aacacctgtg gatagtcgcc
     4561 cgtgatggcg tcgaagttcc ggtttcgctg gtctatcatc gcaaacattt tcgcaaagga
     4621 cacaacccgc tgctggtgta tggctacggt tcttacggcg caagtattga tgccgatttc
     4681 agttttagcc gcttgagttt gttggatcgt ggatttgtct acgccattgt ccatgttcgt
     4741 ggcggcggtg agctggggca acaatggtac gaggacggta aatttctgaa gaagaaaaat
     4801 acgtttaatg attatcttga tgcctgcgat gcattgttaa aactgggcta tggctctcct
     4861 tcgctctgtt atgcgatggg cgggagtgcg gggggtatgt tgatgggcgt tgcgattaat
     4921 caacgcccgg aattgttcca cggcgttatc gcccaggtgc cgtttgttga tgttgtaaca
     4981 acaatgctgg atgaatcaat tcctcttacc accggcgaat ttgaagagtg ggggaatccg
     5041 caggatccgc aatattacga gtatatgaaa agctacagcc cgtatgacaa cgtcaccgca
     5101 caggcttatc cgcatttact ggtaacgacc ggtttacacg attctcaggt gcaatattgg
     5161 gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa ccgataacca tcttttattg
     5221 ctctgtaccg acatggactc aggccatggc ggtaaatctg gtcgctttaa atcgtacgaa
     5281 ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc agggaacatt acccgctacg
     5341 cctgcggatt aagtattttc cagataatgt ttcagtgtta aacgcagctc cgggctcatg
     5401 ctgtccaggt tattaaataa ccagcgcaga tagcccggat cgcgttcggc aacgtcggaa
     5461 accgctttgc cacggtattt gccaaaggtg aaagtcgtca tcaacgatgg acgtccggtg
     5521 atatcggcca tctgttctgc cgtccagccg gaggtgttca taatatcgat aagcaacgca
     5581 gcggtgatat aacagtcata caacgcgcgg tgatgatgca ggcccggcgg cgtctgtaca
     5641 ttgagcttcc gtgttttata taacgccata ttgctgtact tgatccctgg ccacaaacga
     5701 cgggccagtt tcatagtgca aatccactca ccgggcattt caggcagtac gcggcggtca
     5761 aagctggcgt tatgcgcgac ataccattca ctaccgtaat agtgcgggat cacatcttca
     5821 atccacggtt tatcggcgac catggcttcg gtgatgcgat gaatcgccat cgcctgtgga
     5881 ctaataggac gatcgggacg caccaggtgg ctcatgggat tgacgatttt tccgtcaatg
     5941 acatcaacag aggcaatctc aacgatccct ccctgcaaac cgcaggtttc tgtatcgata
     6001 atgcgcaaca tggcatgctc caggccgaaa aagcctagcg taatggaatg atatcgcctt
     6061 gccaaccctg tgatgaacgc tgaccgacta acaataatga accacgatcg gcgcgaacga
     6121 tgagacgacc atgttcatcc catagtgcac tgttaccacg ggcatttgcc atcagcacag
     6181 caatcgagta ctgatgtgaa aaaaattgta agcgacgggc cgacgccagc aggtcgggtt
     6241 ctcccagaca ttgccctgtc gtaaacagtg aacaggtcgg gtccatatcc ataccttgcg
     6301 gttgttcatc gaccaccgtg atggttctgg aacgacggcc cagacatgca ccataactct
     6361 gatggtagat ccctggcgtt tttcgccacg gtgcgaatac cgcaattcca cgaataaagc
     6421 gatcgttata ttcaacggga aggccggcaa tgatggtcat tcgccaggtc gttgcggcat
     6481 aacaaagcgg gtctaatagc gagagatcgg gtggggcagg aagggcacgt cgcgaataat
     6541 cacaccccag taaagaaagc gatggaaaaa ctaacagctg acattgttgc ctggcggcgg
     6601 ccctcacaaa ctccagatga tgagctacct gttcggtgag tgaggtttta cgaggctcat
     6661 attgcgctgc tgcaactttc cagaacgaca taatgacttc cttttcaaaa tgactgcaaa
     6721 ttatgggggc ggtttagaaa atactcataa tttagcaggc attatgtaag aaagtgtaac
     6781 tatgtataag ttttatttaa gtttgggctc gtaaggtaaa cgtgacagat tgaccgaagc
     6841 caaacggtgg gcgataagcc gctcgcgaaa ccagctgcgc aaatgttcag gctgttcacg
     6901 ttcaaccgct tcagcgatca ctggcatatt gtagcgttct ttaaatgcca cccctgccgc
     6961 cgccaaatcg acattcactt tatccatttc tgtttgatcc agtttagcca gattcttcag
     7021 cattcttaat ctcctcatca ttcgcgggca aaagttacta agagtgagcc ttgatggcaa
     7081 gccggaattg atgatcctcg actcagacat tagacaaggt tatgctctgt aaaaaagtta
     7141 ttacaaaaag gaatagataa tatggcttca actgcacgct cccttcgcta cgcgctggca
     7201 atcctgacca cttcgttggt gacaccttcc gtctgggcac atgcgcattt aacgcatcag
     7261 tatcctgcgg caaatgcaca agtgacagca gctccgcagg caatcacttt aaacttctcg
     7321 gaaggcgttg aaacaggatt cagtggcgca aaaatcacgg ggccaaaaaa cgaaaatatt
     7381 aaaacattac cggcgaagcg taatgagcag gaccaaaagc aactgattgt tccactggcc
     7441 gattcgctga aacccggaac ctacaccgtt gactggcatg ttgtttcggt ggatgggcat
     7501 aaaacgaaag gacattacac ctttagcgtg aaataaatca tgctggcgtt tacctggatc
     7561 gcgctgcgat ttatccattt cacctcgctg atgctggttt ttggcttcgc aatgtacggt
     7621 gcctggctgg cccccttaac gattcgtcgc ttgctgacga agcgttttct gcgtttacaa
     7681 cagcacgctg ccgtctggag tttgatcagt gccactgcaa tgctcgccgt tcagggcggg
     7741 ttgatgggaa cgggatggtc agatgtattt tcgccaaaca tttggctggc tgttttgcaa
     7801 acgcagtttg gtggcgtctg gttatggcaa attgttctcg ccctcgtaac attgatcgtc
     7861 gcccttatgc aaccgcagaa tatgccacgt ttgctattta tgctcactac cgcgcaattt
     7921 attctgctgg cgggagtcgg tcatgcgacg ctgaatgaag gggtaacggc gaaaatccat
     7981 cagactaatc atgcaattca cctgatctgt gcggcggcgt ggtttggggg attactgccg
     8041 gtgctctggt gtatgcaact catcaaaggc cgttggcgac accaggctat tcaggcgctg
     8101 atgcgttttt cctggtgcgg gcattttgcg gtgatcggcg tactggcaag cggcgtgctt
     8161 aatgcattgt tgattaccgg atttcccccc acgcttacga ctcactgggg ccaactctta
     8221 ttgctcaaag ccatcctggt gatgattatg gtggtcatcg cgctggctaa tcgttatgtt
     8281 cttgtaccgc gcatgaggca ggatgaagat cgtgcggctc cctggtttgt ctggatgact
     8341 aaactcgaat gggcgatagg tgcggtagtg ctggtgatca tcagcctgct tgcgactctc
     8401 gaaccttttt gatggattac gaaaatgatg aaaaaaagta ttctggcgtt tctgttactc
     8461 accagttctg cagctgcgct ggcggcaccg caggtgatta ccgtcagccg ttttgaagta
     8521 ggtaaagaca aatgggcgtt taatcgcgaa gaggtgatgc tgacttgccg accgggtaat
     8581 gctttgtatg tcattaaccc aagtactctc gtgcagtatc ctttaaacga tatcgcacaa
     8641 aaggaagttg ccagtgggaa gactaaagcc caacccattt cggtgattca gattgatgat
     8701 cctaacaatc ccggcgaaaa aatgagtctg gcaccgttta tagagcgagc tgaaaaactc
     8761 tgttaattac ctgaaatagc cttttgattt ccaataaaaa accgcctcag ttctttcacc
     8821 agaacgggcg gtttttaata tttaagctga tgaccaccac gctttttatt gaccattttg
     8881 cacgcaaact ggaaaacctg gcgtcgtcat ctattcttaa agagcaaggc aactaagcct
     8941 gcattaatgc caacttttag cgcacggctc tctcccaaga gccatttccc tggaccgaat
     9001 acaggaatcg tgttcggtct ctttttatct gttaaaagcc agaagcattc ccttcgctga
     9061 ctttatagtc aaccataaca cacactctac tgtttgagtc cagcgttttt taacattctt
     9121 gttaagatta tgtgatcttt agcgcgggag gaaaatattg atgaaacagc ctgcgcccgt
     9181 ttatcagaga attgcgggtc atcaatggcg acatatctgg ctttcaggcg atatacacgg
     9241 ttgtcttgag caattgcgcc gcaaattatg gcattgtcgt tttgatccgt ggcgagattt
     9301 acttatctca gtgggagacg ttatcgatcg tgggccgcaa agtttacgtt gcctgcagtt
     9361 actggaacaa cattgggttc gtgcggtaag aggcaatcat gaacagatgg cgatggatgc
     9421 gctggcatcc cggcagatgt ctttatggtt gatgaatggc ggcgactggt ttattgcgct
     9481 ggcagataat caacagaaac aagcgaaaac ggcgctggaa aaatgtcagc atttgccctt
     9541 tattcttgaa gtacacagcc gcaccggcaa acatgttatt gctcatgccg attatccaga
     9601 tgatgtttat gaatggcaaa aggacgtcga tttgcatcag gtcttgtgga gccgctcgcg
     9661 attaggtgaa cgccaaaaag ggcagggaat tgcaggtgct gatcatttct ggtttggtca
     9721 tacacctttg cgccatcgcg tggatattgg caacctgcat tatattgata ccggtgctgt
     9781 ctttggggga gaactgactc ttgtgcaatt gcaataatta aaaatcaccg tactcctgtg
     9841 caggtcgcca gaaaccatct ataaaatcct caatcggaaa acaaccgcca tggcggatcc
     9901 gttgatcgct catagaataa agacactgct gttccgtgtt gtagacatcc acaacaatat
     9961 cttcacaacc gccatccagg tagcaaacaa aaagtaccag cgcgaacatt tcatccccga
    10021 agtgtggtgc cgtaccgtta agtttaggag agattttaca acgggggaat aacccggaca
    10081 aataacccgc catgccgacg ggttcttttt ggatcaggca agacgcataa tccaggcatc
    10141 agattgttga tcgtaacggg tacggtaaag gacagagtgt tcgccattta gcagggcagg
    10201 gatactggtt tcagcatccc aggcatccag ttgcatacat aaatcggggt ctgatttaca
    10261 aggaatggtt aacgttcgat cacccggcga ttcgccgtgt aattcgccgt cgtcgatttc
    10321 aaaagcgcct atgcgcacac tggttttcat cttcgtgctc tccatttagc tgccggttgt
    10381 ggtacgacca gtccgatcgc accatttttc agcctagttg aggcagtcga atgtgccagt
    10441 caaaaagtgc gcttttttgt gcgctgtcag gcgttaccgg taaaaagttt cccgtcgcgc
    10501 accagttcac gcgggtagct gtttttcagt cgtgaaccaa tccgtttggc taaaccaatc
    10561 ggctgatgct ggaaagtaac caatacatca tccgccactg gcgcggcatg cgggtataca
    10621 tcgcgcccgc gataccactc ctccgcttcc tgcggtgtca gttcaaaagc gttcacattg
    10681 tcgggggagg caagggcaat aacggcctca tgctgccagc gataaccttt gttgtgcgtt
    10741 tcggcaagtt taatccccaa gcgagaaaat cggactttac cgatcagggc ttcaatgccc
    10801 accgggaaca accacagttc tttgtcacgc tgccagagtc gcaggttttc atcccagttt
    10861 aagccaacac ttgcagccgc ctgacgaatt tgaccggctt cgcgatcttt cactgggcta
    10921 aacgggaaat tgcccacttt gtattttggg gcgggtaaga cggggatcgc ctgagtttta
    10981 cgcagacgag caacgaagaa gccttcgcag tcgtaaattt gtgggaaaac atgcaaaaag
    11041 ccttcctcgg tcagcgcctt gtttgcacca gggaagagat cgccaagcgg taaaaactct
    11101 actgcgtcgg ggtaagtctc tttcagccac aggcaaacgg cttcgttttc ttcccggttt
    11161 aaggtacagg tcgagtaaac cagcgtaccg ccaggacgta atgcatgaaa ggcgctgtcg
    11221 atcagctccc gttgggtcgc tgcgatttcc tgattgcttt ctggtgacca gttttttagc
    11281 gcatcgggat ctttacgcac cacgccttcg ccggagcagg gagcgtccag caaaatggca
    11341 tcgaacattt ctggcactgc cgcaccaaat acgcggccat caaaatgcgt gagcgcaaca
    11401 ttactgatgc cacagcggct gatattggca tgtaacactt ttacccgact ggcggaaaac
    11461 tcattggcaa ggattgcccc ttcgttattc atccgcgcgg caatttgcgt cgttttggaa
    11521 cctggtgcag cagcgacatc catcacccgc tgtggtgcat taccgtcagc aaacaaggcg
    11581 gcaacaggca acattgaact ggcttcctga atataaaaca ggccgcttaa atgctcggcg
    11641 gtactaccca acggcaatgc atcttcattg tcgcgttcaa tccagaaacc ttcttcacac
    11701 cacggaattg gcgtaagcgt ccagccataa ggagcggtta attgcaggaa atcagcaacg
    11761 gaggttttca gcgtattaac gcgaatgctg cggcgcaacg ggcgctgaca ggcggcaaga
    11821 aaatcatcaa aagagagcgt cgaaggcatc gcttcgcgca tttgtgtcag aaaggcgtcc
    11881 gggaaataaa cggtgtgttg ggccacgagc atataccact ggcaaaaaaa caggcgtgca
    11941 gtttagcata aacgctccgg cgcaggcacg ccggagcagt gggcattatt tgggaagcgc
    12001 agttccccat tcacgccact ctttcggttc actttcctgc aacaggaagt gcttgccttc
    12061 ctgggctttc ggtgccagtg gcgtacccgg aggcgtggcg aaggcgatgc cgccacggat
    12121 aaactggtta aaggtgccgg ttttcactac gccgcccgtc agaccaaagt ccagactgta
    12181 acccgatgcc aaccagaaga cggaattgtt acgcaccagg tgttgatagc gtttactgat
    12241 gcgcatcgca atcatcacgc gatctgacaa tgtccccagc gtcattccgg taaccgtacc
    12301 tacttccaga ccacggaaca gcacaggtgt accgatacct aacgaaccgg cttccggcgc
    12361 ttcaacaata atgcttaagc catccaggta acgcgaatca gtaatggtgg cctcttgtaa
    12421 ttcaaagtcg cggcgaggat tgccccggcc tggttcgacg ttgatatacg gctggaggat
    12481 agtatcaaga tgctcaacgc cagctgccga aatttgcggt gtgaccactg agaagcgcgt
    12541 accaccgcga gcaaaggtct ggacatattc cggatagagc accgcctttg cctgtacttc
    12601 attgcgcgcg gtaatcagat ccagcgtctg gatttgcccg atatcaatac cgagatagcg
    12661 aattggcatc ccgaccgcca gttttccggc atcgaaagcg tgaagcgtaa tttgcccgcc
    12721 aaccgcacgg gccgctgttt cggaagcata cagaatacgc ttgtcgcctt tacgctgact
    12781 ggcgctggca ccgctgaggt tatcgaagct aatagccccc tttaatgctc tggagagcgg
    12841 ggatgcctgt acggtcaggc cactaccatt cagctgaact ttcgccccac cttctgccca
    12901 gaacacgctg ttgctggtca gaaggttgcg atactccggt ttaatatgca gatcgatatc
    12961 aaacgcgtta gctcgcggac gcacagtaat aacttcacca acttcaaatt tacggtacag
    13021 cactaccgat cctgcctgca catccggcag cgtctctgca ctcaaactca cggttgtggt
    13081 gggtaaatcg ctaaggctgt tctccagcgc tttttccaga ttggcataca gtggatagct
    13141 ggctttcatc tcgcctttat cgcccggcag aatacgtatt ccgccattga tccattctga
    13201 ggcgctggca ccgagaaact caacgccatc cagccccacc ttcacgtcga cacggctgtt
    13261 gacgacaaat ttgctatcgc cttttaccag ttcgcgatgc tgaggctcga tggcgacggt
    13321 aaaggtgacg cctttgctgg tgagtttacg atcgataacc tggcctacct gcacgccgtg
    13381 aagaatgagc ggctgaccag catcaatacc gtaactttct ggtgctgtca gggtcagtgt
    13441 cagaacatcc ggctcttgca gcagggcttt ttcgcctggc acaaccacga actccttacg
    13501 tggctcgcca tcgccgggca ccagttcgaa ggttttgccg gtcagcaggg cactgagatt
    13561 agcatcgcta agggataatt ttggattacg taattcgatg cgggtatttt cacgaagcag
    13621 ggtgacgacg ctgggatcaa cggtcatttc cccagtgact ttaccaccag gatttaaatc
    13681 cagtttagtc agctgtccga cttccagccc ctgatacatt aacggcgtcg agtcggcggt
    13741 taatccggca ccgcctggca gttccagttt tattattacg ccacgttggc tatgggccag
    13801 gtcttcatac agaccaaagg tatcttccgc ttcggcaggt ttcgactctt caggtgaatc
    13861 gaaggcaatc gcaccgttaa ccagtgccgc cagactttcc agtttcacct tcgcgccact
    13921 gatactgacg ttggcatcaa cgccggaaac gttccagaaa cggctacctt ttttcaccag
    13981 atcggtaaaa cgccgctcga tcaggacatc aatcaccacg ccttgcttgt tgggattgat
    14041 ggcatagtcg tagactttac ccaccgggat cttgcggaaa tagaccaatg aaccactgct
    14101 cagtgaaccg agatcggggg cttgcaggtg gatcatcaga tcgccattgt ccagccgata
    14161 tttcggttgg gtgtcgagtg cgacaaagtg atcctgctct ttacctttac ccggcatcat
    14221 gccgatataa ttaccaccga cgagggcgtc cagcccggag acacctgcca acgaggcctt
    14281 tggcgtcacc aaccagaact gtgtctcttc gcgcagcgca tctttcatat cggacttgat
    14341 gctgaccttg acttcaatct tacgaagatc gtcgctgagg ctgatatcct gcactgttcc
    14401 aacttcaacg ccctgataac gaacaggcgt acggcctgga acaataccat ccgccgacat
    14461 aaagtcgatg gtgacagtat tgccccggtc ctgataactg tcccaaatca gccaaccagc
    14521 aatcattagc gcgatgaacg gcagcagcca gaaaggtgag atacggcgtt tatttttaat
    14581 ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc
    14641 tgtccagcca ttccacagca agaatagtca aaattaccgc tgcgccgaaa taaaacgcag
    14701 ccggtcccat agtaaaagcg aggatctgat cgcgattaat cagcgacatg gttaaagata
    14761 tgacaaacag gtcgagcatc gaccagcgac caatccaggt caccatccgc agtaacagaa
    14821 tgcgtgtgcg taaaccttgc tggcatttaa aatgaatact caacagtaaa gtgaacatga
    14881 cgatcacttt agtaaacggt accagaatac tggcgataaa cacgattccc gcaactgcaa
    14941 tgttgctact tgccagcgac ataattccgg aaagaatcgt atcttcctgc cgaccaccat
    15001 tcagataaat gatagagata ggcaacaggt tggcaggtaa caacaaaacg attgacgcta
    15061 acagcgccgc ccagcatttt tgcagactat gacggcgacg caggagtagc gggatatggc
    15121 aacgcgggca gcgaccacgc tgatcaggat agccggtaaa atggcaccca agacagacac
    15181 ggagtttctc gtcccttcgc gtagcggggc gctgcggata aaatcgctcc cacagttctt
    15241 cgacattaag atgtgacaac gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca
    15301 agccgacccc cgcctggata tgggcataat cctgtacctt tatagaagca acgccaatgc
    15361 cgaccaggta gatatccaac atcacccact ctttcagccg ctcaagcatc agcagtaccg
    15421 ggcgtaaatt cattcccaat cggttaccaa accataaata agctatggag gtcaccagaa
    15481 tgaggggggc accgataacg cagaaaaaga ccatcgcccc cgttatcgca tcgccctgtt
    15541 tggtcatttg ccagatgcct tgcataacgt tggcgtcaat acgaatgcct aacagccaga
    15601 tatgcaacag cggttcgccc caggcaaacg gcataagcaa cagcatggtg aaggccattg
    15661 ccgccaggcg cgttagcgac cagtcgcgcc cgtcacgaat ttttgcctga cagcgcggac
    15721 aataggcact ttgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc
    15781 aacgttggta atcaccacgc ggcagttcct cgccgatagc cctcactgtt atcttctttg
    15841 tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat
    15901 taatcttaac ccatgatggg aatgatcttg agcattaacg catttaatgc ttattttaat
    15961 aggctggcag ttccaggtaa gacaaccaag tgattatata atgaacaaag cagaatttta
    16021 cgcggattta aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac
    16081 gcttgcgaac accagtgcgt tgttatatga gcgtctcact gacgtaaact gggcaggttt
    16141 ttatttgctt gaggacgata cactggtgct cggaccattt cagggcaaaa ttgcctgtgt
    16201 ccggataccc gttgggcgcg gcgtatgcgg cactgcggtt gcccgcaatc aagtgcagcg
    16261 tatcgaggat gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga
    16321 aattgttctg ccactggtgg tgaaaaatca gattattggt gttctcgaca tcgatagtac
    16381 cgtcttcggt cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct
    16441 tgaaaaagtg cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc
    16501 aacggataac gtagcattta ctgatagcgt cattataatg acgcctgttc atgcctgcgc
    16561 ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag
    16621 cagtaaagaa gtaatcgcgt ttctggccga acgttttccc cactgtttca gtgcggaagg
    16681 tgaagcgcgt ccgctgaaaa tcggtatttt tcaggatttg gtcgatcgtg ttgccgggga
    16741 aatgaacctg agcaaaacgc aattgcgatc cgctttacgt ctctacactt cgagctggcg
    16801 ttatctttac ggtgttaaat ccggcgcaac gcgtgttgat cttgacggca acccatgcgg
    16861 tgagctggac gagcaacatg tcgagcatgc tcgcaagcag cttgaagaag cgaaagcgcg
    16921 tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg
    16981 tgagaaagaa gacgcaccgc gccgcgaacg caagccacgc ccgaccacgc cacgccgcaa
    17041 agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt
    17101 aaaagcacct cgcgaagaac agcacacccc ggtttctgac atttcagctc tgactgtcgg
    17161 acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac
    17221 caaagacggc gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca
    17281 cctggtgttc tgaaacggag gccgggccag gcatgaacat gttttttagg cttaccgcgt
    17341 tagctggcct gcttgcaata gcaggccaga ccttcgctgt agaagatatc acgcgtgctg
    17401 atcaaattcc ggtattaaag gaagagacgc agcatgcgac ggtgagtgag cgcgtaacgt
    17461 cgcgcttcac ccgttctcat tatcgccagt tcgacctcga tcaggcattt tcggccaaaa
    17521 tctttgaccg ctacctgaat ctgctcgatt acagccataa tgtgctgctg gcaagcgatg
    17581 ttgaacagtt cgcgaaaaag aaaactgagt taggcgatga actgcgttca ggtaaactcg
    17641 acgttttcta cgatctctac aatctggcgc aaaagcgccg ttttgaacgt taccagtacg
    17701 ctttgtcggt actggaaaag ccgatggatt tcaccggcaa cgacacttat aaccttgacc
    17761 gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa cgcgctgtgg gacagtaaag
    17821 tcaaattcga cgagttaagc ctgaagctga caggaaaaac ggataaggaa attcgtgaaa
    17881 ccctgactcg ccgctacaaa tttgccattc gtcgtctggc gcaaaccaac agcgaagatg
    17941 ttttctcgct ggcaatgacg gcgtttgcac gtgaaatcga cccgcatacc aactatcttt
    18001 ccccgcgtaa taccgaacag ttcaacactg aaatgagttt gtcgctggaa ggtattggcg
    18061 cagtgctgca aatggatgat gactacaccg ttatcaattc gatggtggca ggaggtccgg
    18121 cagcgaagag caaagctatc agcgttggtg acaaaattgt cggtgttggt caaacaggca
    18181 agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt ggtggcctta attaaagggc
    18241 ctaagggcag taaagttcgt ctggaaattt tacctgctgg taaagggacc aagacgcgta
    18301 ctgtaacgtt gacccgtgaa cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga
    18361 agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc aggcttctat gtgggtttga
    18421 cagacgatgt caaagtgcaa ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatta
    18481 tcgacctgcg tagcaatggt ggtggggcgt tgactgaagc cgtatcgctc tccggtctgt
    18541 tcattccatc cggcccgatt gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca
    18601 gcgacaccga cggacaggtc ttctataaag gcccgctggt ggtgctggtt gaccgcttca
    18661 gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga ttacggtcgt gccctggttg
    18721 tgggtgaacc gacgtttggt aaaggcaccg ttcagcagta ccgttcattg aaccgtattt
    18781 acgatcagat gttacgtcct gaatggccag cgctgggttc tgtgcagtac acgatccaga
    18841 agttctatcg cgttaacggc ggcagtacgc aacgtaaagg cgtaacgcca gacatcatca
    18901 tgccgacggg taatgaagaa acggaaacgg gtgagaaatt cgaagataac gcgctgccgt
    18961 gggatagcat tgatgccgcg acttatgtga aatcaggaga tttaacggcc tttgaaccgg
    19021 agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc tgagttccag aacatcatga
    19081 aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa tatcgtttct ctgaattacg
    19141 ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac
    19201 gctttaaacg cgaaggtaaa ccggagttga agaaactgga tgatctaccg aaagattacc
    19261 aggagccaga tccttatctg gatgagacgg tgaatatcgc actcgatctg gcgaagcttg
    19321 aaaaagccag acccgcggaa caacccgctc ccgtcaagta atatctatca ggcacaagaa
    19381 attgtgcctg attttttaac agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa
    19441 gttgtgtctt tctggtgact tacgcactat ccagacttga aaatagtcgc gtaacccata
    19501 cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg
    19561 aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc gttttcgggc tggtactgag
    19621 cctgacaggg atacagtcga gcagcgttca ggggctgatg atcatggcct tgctgttcgg
    19681 ttttggtggt tccttcgttt cgcttctgat gtccaaatgg atggcattac gatctgttgg
    19741 cggggaagtg atcgagcaac cgcgtaacga aagggaacgt tggctggtca atactgtagc
    19801 aacccaggct cgtcaggcgg ggatcgctat gccgcaagtg gctatctacc atgcgccgga
    19861 catcaatgct tttgcaactg gtgcgcgccg tgatgcctct ctggttgctg tcagcactgg
    19921 tttgctgcag aacatgagcc cggatgaagc cgaggcggta attgctcacg aaatcagcca
    19981 catcgccaat ggtgatatgg tcaccatgac gctgattcag ggcgtggtga acaccttcgt
    20041 tatctttatt tcccgtattc tggcgcagct tgccgcgggt tttatgggcg gaaaccgtga
    20101 tgaaggtgaa gagagcaacg gcaacccgct gatctacttt gcggttgcaa cggttctgga
    20161 actggtgttt ggtattctgg cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt
    20221 ccatgctgat gcgggttcag caaaactggt tggtcgcgag aaaatgattg ccgcgctgca
    20281 acgcctgaaa accagctatg aaccgcaaga agcaaccagc atgatggctt tctgcattaa
    20341 cggtaagtcg aaatcgctca gtgagttgtt catgacccac ccgccgctgg ataaacgtat
    20401 tgaagctctg cgtacgggtg aatacctgaa gtaatatgac gtaaatacta aaaagcgcgt
    20461 cctgatcgac gcgctttttt tatgccctgg atcgtggctg agtgatacgt aaaccactga
    20521 cacaggcagc aatcactgcc agaatagccg cagctatcag cgagacgtgc gtaccattat
    20581 caccaaactg atttagcatc agcgctacca gagccgcgcc gctactctga cccagaagac
    20641 gagccgtccc taacatgcca ctggctccac cgctacgttc gcgaggagcg gaggtaataa
    20701 tggtgtggtt attgggtgac tggaacaagc caaatccagc accacataag atcatcggcc
    20761 agataatatt gatatccgca ggtgacgctg gcagcagaac cagggaaaaa agccccgcag
    20821 ccatgatgaa taaccctaaa gcacccagca atcctgcatg tacgcgttca atcaaatagc
    20881 ctgccagtgg agccataacc atcgttgcta acggccacgg tgtcagaagt aaacctgttt
    20941 cgacttcact acgcccgagc acggtttgca ggtaaaaggg aagggaaacc attgccagca
    21001 tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca
    21061 gtaaatccac cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac
    21121 caacgacaac cattaccacc agttccgcac caatcaatgt cagcgattgc ccctgagcga
    21181 aaccactcaa tgcagtaata agcaggccga aggttaacgc gttcatcacg gcgctgggca
    21241 ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa
    21301 gcagggcgat aatacccaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg
    21361 agaggattgc tgcagcaatt gtcggcccgg cagcagaaga gacggcaaca ataaacgagt
    21421 ttatgcccat ccctctaccc agaaaacgtt gtggatagat caggcggata agtgcggtat
    21481 taacgctcat caacgctgca ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt
    21541 gcagcgaatc agaaagggcg cagaacagtg aagacaacag aaaaacgacc agaccgcatt
    21601 tataaatacg tcgatagcca aacatatcgc ccagaaacga aaacgagagc agggagatga
    21661 caatggcgat ttgataggcg ttcactaccc agatggaact ggccggcgtg gcatgaaggt
    21721 ccgtagcgat tgttggcaag gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa
    21781 taccaatcac aatggttaat atcgcaccgt atcgcagggg caatggcagg ccgtcggcct
    21841 gaacttttgg cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag
    21901 cactgatatt cacggtggat gtcgcagaat tgtaactaac cggcaaaaga t
//
LOCUS       DAOFSV010000065        21811 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.065, whole genome shotgun sequence.
ACCESSION   DAOFSV010000065 DAOFSV010000000
VERSION     DAOFSV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.065"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..149)
                     /gene="hokE"
                     /locus_tag="QBH27_002639"
     CDS             complement(<1..149)
                     /gene="hokE"
                     /locus_tag="QBH27_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDQ7014934.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPK"
     gene            complement(499..651)
                     /gene="hokE"
                     /locus_tag="QBH27_002640"
     CDS             complement(499..651)
                     /gene="hokE"
                     /locus_tag="QBH27_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDQ7014935.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            1094..2212
                     /locus_tag="QBH27_002641"
     CDS             1094..2212
                     /locus_tag="QBH27_002641"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDQ7014936.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FISLSAASPYMQGTDTRFASSRPNIFSAFPDNGPMQWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            2278..2526
                     /locus_tag="QBH27_002642"
     CDS             2278..2526
                     /locus_tag="QBH27_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDQ7014937.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2591..2974
                     /locus_tag="QBH27_002643"
     CDS             2591..2974
                     /locus_tag="QBH27_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDQ7014938.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            3053..3706
                     /gene="nfsB"
                     /locus_tag="QBH27_002644"
     CDS             3053..3706
                     /gene="nfsB"
                     /locus_tag="QBH27_002644"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDQ7014939.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3814..5061
                     /gene="ybdG"
                     /locus_tag="QBH27_002645"
     CDS             3814..5061
                     /gene="ybdG"
                     /locus_tag="QBH27_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDQ7014940.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5219..6601)
                     /gene="pheP"
                     /locus_tag="QBH27_002646"
     CDS             complement(5219..6601)
                     /gene="pheP"
                     /locus_tag="QBH27_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDQ7014941.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(6703..9846)
                     /gene="cusA"
                     /locus_tag="QBH27_002647"
     CDS             complement(6703..9846)
                     /gene="cusA"
                     /locus_tag="QBH27_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDQ7014942.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVSDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9858..11081)
                     /gene="cusB"
                     /locus_tag="QBH27_002648"
     CDS             complement(9858..11081)
                     /gene="cusB"
                     /locus_tag="QBH27_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDQ7014943.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEIDNADEALKPGMNAGLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11097..11429)
                     /gene="cusF"
                     /locus_tag="QBH27_002649"
     CDS             complement(11097..11429)
                     /gene="cusF"
                     /locus_tag="QBH27_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDQ7014944.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11453..12835)
                     /gene="cusC"
                     /locus_tag="QBH27_002650"
     CDS             complement(11453..12835)
                     /gene="cusC"
                     /locus_tag="QBH27_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDQ7014945.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDITEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12992..13675
                     /gene="cusR"
                     /locus_tag="QBH27_002651"
     CDS             12992..13675
                     /gene="cusR"
                     /locus_tag="QBH27_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDQ7014946.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13665..15107
                     /gene="cusS"
                     /locus_tag="QBH27_002652"
     CDS             13665..15107
                     /gene="cusS"
                     /locus_tag="QBH27_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDQ7014947.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            15257..17494
                     /gene="nrfB"
                     /locus_tag="QBH27_002653"
     CDS             15257..17494
                     /gene="nrfB"
                     /locus_tag="QBH27_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDQ7014948.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            17481..20453
                     /gene="nfrA"
                     /locus_tag="QBH27_002654"
     CDS             17481..20453
                     /gene="nfrA"
                     /locus_tag="QBH27_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDQ7014949.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPASLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALTAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WSFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            20454..21344
                     /locus_tag="QBH27_002655"
     CDS             20454..21344
                     /locus_tag="QBH27_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDQ7014950.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISIFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         5291 a         5865 c         5431 g         5224 t
ORIGIN      
        1 ttcttcggtt cgtaagcgag aacagcctta aactcaatat tacgttcctt tacggtaaac
       61 tcacacagcg agtctccgac cagaagcgta aatcccagca ccgtcaaaca cagcactatg
      121 actgccacaa gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact
      181 accatccaaa ttgtctggtt tggagtggca gccccagggt tgatagaaat aactcctggg
      241 gcttcatctt tccagacctc aaagttaacc tgaaaccgga aagcttcagg cacccgccgt
      301 tatcttaccc ttcctcaaca aaatagctgt ttatttatac agtaagtcct gtttcggact
      361 tctgttggca aaactgatca ttgcccttaa aacgacatcg cccgcagcca tcactgaccg
      421 cgggcgaaag tgtaaagcag gtgccttacc accctgacct gacaaccgga tatgcgggga
      481 ttactccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat
      541 attacgttcc ttcaccgtaa actcacacag cgagtctccg accagaagcg taaatcccag
      601 caccgtcaaa cacagcacta tgactgccgc aagggcatat ttcgtcagca tgtttatatt
      661 gcctccatgc gaagaaggga ctaccatcca aattgttcag gtttgggtag gtagccccag
      721 ggttgataga aatatcgtcc tggggcttca tctttccaga cctcagagta aacctgaaac
      781 cggaaagctt caggcacccg cagttatctt acccttcctc aacaaaatag ctgtttattt
      841 atacagtaaa tcctgtttcg gacttctcac tccccattta atcaactgaa gctgaacccg
      901 tttttttctg catgcctcaa tcgtcaaaat tcttcgccgc cagccagctc tgccataact
      961 tgtcacattg caaaatgtag ccaatttgct tcagtcacca tcaccaaaaa aagaaacatt
     1021 ccagaacttc cgcatctcgc ccagccgctc ccacttctat actgaataga aaacgccaac
     1081 ataagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta ccctcggtat
     1141 tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg actcatcaat
     1201 gctgattgac acggttaaaa atcagatcac ggccggagag gtaaagcacg atatcaccga
     1261 aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg ccgggcaatt
     1321 ttcagcgatg cagaaagtcg tattgcaggc agcggcagac catcatctgg aaatttgcgg
     1381 cggtggcacg catccgtttc agaaatggca gcgtcaggag gtatgcgata acgaacgcta
     1441 tcaacgaacg ctggaaaact ttggctatct catccagcag gcgaccgttt ttggtcagca
     1501 tgtccatgtt ggctgcgcca gtggcgatga cgccatttat ttgctgcacg gcttgtcacg
     1561 gtttgtgccg cactttatct ccctttccgc cgcgtcgcca tatatgcaag gaacggatac
     1621 gcgttttgcc tcttcacgac cgaatatttt ttccgccttt cccgataatg gcccgatgca
     1681 gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgcctgagtt acaccacgat
     1741 gatcgacagc attaaagatc tgcactggga tattcgcccc agcccccatt ttggtacggt
     1801 ggaagttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata tggcgggatt
     1861 aattcaggct accgcccact ggttactgac ggaacgcccg tttaaacatc aggagaaaga
     1921 ttacctgctg tataaattca accgtttcca ggcctgtcgc tatgggctgg aaggtgtcat
     1981 taccgatccg catactggcg atcgtcggtc actaacggaa gccaccttgc gtttactgga
     2041 aaaaatcgcc ccttctgcac ataaaattgg cgcatcgagc gcaattgaag cactgcaccg
     2101 tcaggtcgtc agtggtctga atgaagcgca gctaatgcgt gatttcgtcg ccgatggtgg
     2161 ctcgctaatt gggctggtga aaaagcattg tgagatctgg gccggagaat aagtcgggaa
     2221 ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa caatgttatg
     2281 aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc tttcctctct
     2341 tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact ggtgaccacg
     2401 ttccatctga tggatcttag ccagctttac acgttattgt tttgtctgtg gtttttagtg
     2461 ctgggcgcta ttgagtattt tgttctacgc tttatctggc gacgctggtt ctcgctggcg
     2521 gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc cttcgcctaa
     2581 aggaacattt atggataagc aatcactgca cgaaacggcg aaacgcctgg ctcttgagtt
     2641 accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta aaattggcgg
     2701 caagattttt atgctgtcgt cggagctgcg cggcgtcccc tttattaatc tgaagtccga
     2761 tccacaaaaa tccctgctaa atcagcaaat atacccaagc atcaagccag ggtatcacat
     2821 gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag cgttacttcg
     2881 cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac gcgatcaaaa
     2941 acgactgcgt cctgactcaa ccgtaaatca atagcgcatt tttctcgctt accatttcat
     3001 ctcgatcctt gtaatctgct ggcacgcaaa attactttca catggagtct ttatggatat
     3061 catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca aaaaacttac
     3121 cccggaacag gccgagcaga tcaaaactct actgcaatac agcccatcca gcaccaactc
     3181 ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg ttgccaaatc
     3241 cgctgccgat aattacgtgt tcaacgagcg taaaatgctt gatgcctcgc acgtcgtggt
     3301 gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg accaggaaga
     3361 tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag gtcgcaagtt
     3421 cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg caaaacaggt
     3481 ttatctcaac gtcggtaact ttctgctggg cgtggcggcg ctgggtctgg acgcggtgcc
     3541 catcgaaggt tttgacgccg ccatcctcga tgaagaattt ggtctgaaag agaaaggcta
     3601 caccagtctg gtggttgttc cggtgggtca tcacagcgtt gaagatttta acgctacgct
     3661 gccgaaatct cgtctgccgc aaaacattac cttaaccgaa gtgtaattct cccttgccgg
     3721 gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg tttcagccgt
     3781 catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc ccaggttgaa
     3841 gatttagcgg gtattgagat tgatcacacc acctcgatgg tgatgatttt cggtattatt
     3901 tttctgaccg ccgtcgtggt gcatattttt ttgcactggg tggtactgcg gaccttcgaa
     3961 aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa taaactcttc
     4021 caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt attctggcta
     4081 caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg gatcatgatg
     4141 tatgcgctgc tttcagtctt ctcgttgctg gatgtgattt tgaatctggc gcagaaattc
     4201 ccggcggcat ctcagttacc gctgaaaggg atttttcagg ggattaaact gatcggcgcg
     4261 attctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc gattctgatc
     4321 agtggtcttg gtgcaatggc tgccgtgcta atgttggtat ttaaagatcc gattcttggt
     4381 ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggtga ctggctggag
     4441 atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac cgtcaaagtg
     4501 cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc tgactccttt
     4561 aaaaactgga gcgggatgtc agcttctggc gggcgacgta ttaaacgcag tatcagtatt
     4621 gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgcctgaa taaagcgcat
     4681 ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa tcgccagcaa
     4741 ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac ctttcgtgcc
     4801 tatctgaacg aatatctgcg gaatcatccg cggattcgca aagatatgac cttaatggta
     4861 cgccaactgg ctcctggtga taacggttta ccgcttgaga tctatgcgtt taccaacacc
     4921 gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat atttgcgatt
     4981 gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat tcgctctctg
     5041 gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg agagcggctt
     5101 tcagattgtt gacatcattt tgtcggatgc ggcgtgaacg ccttatccga cctacagaat
     5161 cgttacaaat tgtaggcccg ataagcttgc gcatcgggca aatcagcctc acatcagctt
     5221 aattcacttt cctacgcaat gttttaaacg ccacaaacag gaacacaatc cacaccggca
     5281 gcagaatcgc tgacaagcgc atatcatcca tcgtgcacat cagcagcaaa atcatgccga
     5341 ggaaggcgat gcaaagatag ttgccgaacg gatagagcag cgccttaaat tgtgtttcac
     5401 gcccctgacg tcgcatcgcc gcgcgaaaac gcagatgcgc caggcagatc ataatccagt
     5461 tcaacagcag cgttgctacc accagcgcca tcagcagacc aaacgctttt tgcggcagca
     5521 gatagttgat taacaccacc agcgaagtga tcgctccgga aagcatcagc gagttaatcg
     5581 gcacgccgcg acggctgacg cgagtcaaaa actttggcgc attaccctgc acagaaaggc
     5641 caaacagcat gcggctgtta gagtaaaccc cgctgttata caccgacagc gatgccacca
     5701 gaatgacgaa gttcagcgca gaagctacca cgttgctgtc gagattatgg aaaatcatca
     5761 caaacgggct actattggat ttcacttcca cccacgggta gagcgccagt aaaaccacca
     5821 gtgaaccgat ataaaacagc aggatgcgat acaccacctg attcactgct ttagggatgc
     5881 ttttttccgg atcgcgcgct tcagccgcag taatcccaat cagctccaga ccgccgaagg
     5941 agaacataat taccgccagc gacaaaatca gtccattcca gccggtggca aagaaaccgc
     6001 cgtagcgcca gaggttatcg atactggctt tctcaccgcc gtgcccagaa aacagcagcc
     6061 acaggccaaa gccgatcata ccgatgatcg ccagcacttt aatcagcgca aaccagaact
     6121 cggtttcgcc atataagcgc acgttcacca ggttaacggc gttgatgata ataaagaagg
     6181 cggcagccca aatccacgtt ggaacatccg ggaaccagta ctgcatatag atgcccgcag
     6241 cggtcagctc tgccattccc accagcacga acattaccca gtagttccag ccagagagga
     6301 agcccgcaaa cggtccccag tatttatagg caaagtgggc aaatgaaccg gataccggct
     6361 cttcgaccac catctcgccg agctggcgca taatcaggaa agcgatgatc ccggcgacgc
     6421 cgtagcccag caatacagcc ggacccgcca tctgaatcgc cgggccaatg ccaagaaaca
     6481 gaccagtacc aattgcgcca cccagcgcaa tcagttgaat atgacggtta tgtaatccgc
     6541 gatgaagcgt cggctcttga ttcgacgcag tatcttccga tacggttgac gcgtttttca
     6601 cgcctttccc ctgtgtgtct tttttgttga ggggcacctt ttaacattta gtgcccatcg
     6661 tcgcaagaca caatccacac ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg
     6721 tcggtgcagc cacatcagct tatacgccgc cgggataata aacagcgaca gcaaaggcgc
     6781 ggtgatcatg ccgccaatca tcggcgcggc aatccggctc atcacctctg accctgctcc
     6841 tgttccccac agaatcggca gcagaccagc gataatcacc gccaccgtca tcgcttttgg
     6901 gcgcacgcgc agtaccgcgc cgtgatataa cgcctcatcc agcttctgct cgctgaatgt
     6961 ttgcggatta ttcaacgacg gctcggcctc tatggcgtga cgtaaataca tcagcatcac
     7021 cacgccaaat tcggcggcga ccccggcaag ggcaataaaa ccggtgcccg tcgccacgga
     7081 aagatgaaag cccatccacc acagcaacca gatgccgccc accagcgcaa acggtacgct
     7141 gctgataatc agcaacgctt cgcccacccg acggaacgcc agatacaaca gcacgaagat
     7201 gatcatcagc gtcatcggca ccatcagttt cagcttatgg ttggcgcgct ccagtagctc
     7261 gaactgcccg gagaatgcca cgctggtacc cggttttaac tgcacttttt cagctatcgc
     7321 tttttgcaga tcgtgaacca ccgacaccat gtcacgatcg cgggcatcga tataaatcca
     7381 gctcgtcggg cgcgcattct cggttttcag catcgacggt ccggtagaga ctttaacgtc
     7441 ggccacgtcc gccagagtga tttgctgctt catcggcgtg aggatcggca actggcgcag
     7501 tgcctgcggg ctatcgcgcc agctttgcgg ataacgcaga ttaattggat aacgagcaat
     7561 cccttccacc gtttcgccaa ccatcgcccc gcccaccgca gaagtcacaa acaactgcac
     7621 atccgccacc gtcataccgt aacgcgcggc tttttcacgg ttaatctcaa cgttgatata
     7681 acgcccacct tccagacgct cagcaagcgc agaagccacg cctggcaccg ttcgcgccac
     7741 ttcttcaatt tgctccgcca tcgtgtcgat atccgccagc acagtgccgg aaactttaat
     7801 gccgataggg cttttaatgc cagtcgagag catatcgata cggttacgaa ttggcggcac
     7861 ccacagattc gccagccccg gcagacgcac ggtgttatcc agttcctcaa tgattttgtc
     7921 catcgtcatg ccaggccgcc actgatcctg cggcttaagc tggatagtcg tttctaccat
     7981 ctccagtgga gctgaatcgg tggcggtttc cgctttcccg gttttgccaa atacccgcgc
     8041 cacttccggt acgctcataa tcagcttgtc gtttttttgc agcatgctcg ccgcctctgc
     8101 tgcggaaatc cccggcagcg tcgatggcat atacaacaag tcgccttcat tgatctgcgg
     8161 taaaaattcc ccgccaactt tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc
     8221 caccaacagc gtggttttcg gccagtgcag tactttcagc aacagcggat gataaacacg
     8281 aatcaaaaag cgattgagcg ggttactgct ttccggcgga attttgccac ggatccagta
     8341 gcccatcagg atcgggatca ctacgatcgc cagtagcgcc gcgcccgcca tcgcataggt
     8401 tttggtgaac gccagcgggc caaacagacg accttcctgc ccttccaggg tgaagatcgg
     8461 gataaacgac agcgtgataa tcagcagact gataaacagc gccggcccca cttcaacaga
     8521 tgcatcggtg atcacctgcc agcgcgtttt attatccagc gtggcgtcag gatgctggtg
     8581 ctgccactct tccagccgtt tatgcgcatt ctcgatcatg acgatagcgg catcgaccat
     8641 cgccccgacg gcaatcgcaa tgccacccaa cgacataata ttggcgttca gtccctgaaa
     8701 gtgcatgaca ataaaagcaa tacacaaccc cagcggcaac gaaataatcg ccaccagcgc
     8761 cgagcgcaca tgccagagaa acagcgcaca gactaccgcc accacaataa actcttccag
     8821 caacttgccg ctgaggttgt caatggcgcg gtcaatgagc tggctgcgat cgtatgttgt
     8881 aactatctcc acgccttccg gcagactgct tttcagcgtt tccagtttgt ccttcacagc
     8941 ggcgatcact tcacgggcgt ttttgccgga acgcaggatc accaccccgc ccgccacttc
     9001 gccttcgccg ttcagttcgg caatgccccg gcgcatctcc gggccaacct ggaccttcgc
     9061 aacatcacgc agataaacgg gcacgccatt ttcactggct tttaaaacga tgtgattaaa
     9121 gtcgtcgagc gtttgcagat agccgctggc gcgcaccata tattccgctt ccgccagttc
     9181 tatcgacgaa ccgcccgctt cctggtttga agcatccagc gcgcttttta cgtcggcgag
     9241 actgatgcca tactgcgcca ggcgctgagg atcgctaacc acctgatact ctttcaccac
     9301 accgcccacc gacgccactt ccgcaacgtc agggatggtt tttagctcat atttgagaaa
     9361 ccagtcctgc aatgagcgta aatcggccag atcgtgctta ccgctgcgat ccaccagtgc
     9421 atattcatag atccagccaa cacccgtggc atctggcccc agctcggcgc tgactcccgc
     9481 aggcagctta ccctgtacct ggttgaggta ctccagcacc cgcgagcgcg cccagtacgg
     9541 atcggtgcca tcttcgaaaa tgacatacac ataagagtcg ccaaactgcg agaaaccgcg
     9601 cacagtcttc gcgccaggca ccgacaacat ggtggtggtt agcggataag tcacctgatt
     9661 ttcaacgatt tgcggtgcct gaccgggata gctggtttta ataatcacct gcacatcgga
     9721 aagatccggc agtgcatcca ctggcgtgtt aatgatggtc caggtgcccc agatactcag
     9781 aaacaacgcg cccatcagca ccagaaaacg gttcgccacc gagcgacgaa taatccattc
     9841 aatcattggc tattccctca atgcgcatgg gtagcacttt cagagcgcat ccgctccagt
     9901 gcgccagaaa tattggcttc agaatcaatc aggaacaggc cgctggaaac caccttttca
     9961 ccttccgcca gaccagagcg taacgcggtg acgccttgcg atgcctggaa aacggcaacg
    10021 cgtttcggta caaagcgccc gtcagcatca acggtaatca cccgctgttc gttgccggta
    10081 tcaatcagcg cctgtgacgg aatgagcagc atcggttcgc tggcggtatt gagttgcaac
    10141 ccggcgttca tgcccggttt tagcgcctcg tcggcgttgt cgatttccag acgcaactgc
    10201 agcgtgcggg tcgcggcatc cacgccgggt agcagggtcc atttgcgaat ggtgagtgtt
    10261 ttatcaggtc gcgccggaac ggtgagcgta aactgcgagg catctttcac cagccaggcg
    10321 atggactccg ggatcgcagc agtgacccac accgggtcca taccctgaat tttcgcgacc
    10381 acgttatctt tggcgatatt cattcccgcg cgcagatcaa acgcggtgat cacgccatca
    10441 atgggcgctt tgagcgtaaa gcgagtctgg attttttgcg tggcgatcag gcggcgaata
    10501 tcagcctccg gcattcccgc cagtcgcagt cgctcaagaa tgccttcagt ctgggtcgcc
    10561 gtaccgccgg tttcgcgcag cagtaaatac tcactctgcg cttccaccca gtcgggaatg
    10621 gtcaggtcga gaagcggcgc gcccttttgc actttatcgc ccacggtaag cggatacacc
    10681 ttgtcgataa acccggcggc gcgggcctgc acaatggcat actgatactc gttgtaactg
    10741 acattcgccg ggaaactctg ggaaaaagtc agcggcccgc gcgtgacggt cgccgttttc
    10801 acccccagat tctgcgtctg agtcgggtca atgcgcacac cagacgcaga actctcttca
    10861 tcggcatatt tcggcaccag atccatatcc ataaacggcg atttccctgg tttatcgaac
    10921 cgcgtattgg gatacatcgg gtcgtaccag aataagactt tacgttctgc ggtcgacgtt
    10981 ttttctgcgg gcggttcctc ctttgcaaac caggtaaaac ctgccgcaga aataataccg
    11041 cccgcgatca tgctgccgat aataagcgcg atttttttca tctcattaaa cccggattac
    11101 tggctgactt taatatcctg taataaagaa agattgccct gctggacaaa attaaacgct
    11161 actttatcgc cggttttaat ttcgctcatt ttcgtctgcg gggtgatggt aaagcgcata
    11221 gtcatctccg gccagttcac ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt
    11281 tccagatcaa caccttttac cacgccagtg gcgctaataa cctgtggttg tgcttcgctc
    11341 atggtttcat gatgatgttc gttagcctgg gcattaaagc caataacggt aaacagactg
    11401 aacattgcga cttgcagtgc ttttttcatt tattctctcc tggagttaaa aattattgct
    11461 gccaaccgcc ccctaaggcg gtatataaag aaatttcgtt aacctgacgg gcataattca
    11521 ggtcaagtaa agtttgtcgg gttgcaaata aagaacgttc agcatccagc acttccagat
    11581 aactgactgc gccgtgctga tataatgccc gcgcccgttg cagggtaatt tgcagcgacg
    11641 caagataacg ctgctgggcg ctgatttgat cgttcaggct ttgacgtaat gccagcgcat
    11701 ctgccacttc tttaaaggcg ttctggattt tctgttcata attcaccacc gactgctgct
    11761 ggcgaatttc ggtgatatcc agattggcct ggttgcgtcc ggcattaaaa atggggatct
    11821 caattttagg aataaaattc cacatcccgc tgctggcgtt aaataatgac gacagatcgc
    11881 tgctggcggt tgatattccg ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag
    11941 caccaatatt ggcattagcc gccattaacg cgtgttcagc ttccataata tcagggcgct
    12001 gcaataagat ttgcgacgac aagcccgccg gtaatttaac gctttgcagg ctgtcgctgt
    12061 ttactgtctg cgcttgcggc agcttgccgt agcttcccaa taacagttgc aatgcattat
    12121 tcgcctgcgc cagttccccc tgacgtttag cgatgtcgct gcgggtactt tctatcaccc
    12181 cgcgagcctg ttccagcgcc agaacattgc tgctaccggt caacagttgt ttttcgacaa
    12241 acgcatatga ctgctgataa ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg
    12301 ccagttgctg attgaaatag ctttgcgcga cattagaaac caacagaata tgcaccgcgc
    12361 gctgagcttc ctcagtggct aaataatttt ggcgctcggc ttcgctcatg ttctttaagc
    12421 gaccgaaaaa atcgagatca aagctggcgt taaggccagt cgataactcc cgcgtcgtgg
    12481 ctgaatcgcc tttaagattg ccgctccagc tgccgctgcc ctcgccattg agctgtgggt
    12541 agcggtcggc atcggtcaga cgatattgcg cccgcgcttc ctgcactttc agcgccgcca
    12601 tgcgcaaatc ccggttattc accaacgcct cgctaatcag cgtcttcacc tgattatcaa
    12661 caaaaaaggt gcgccagccc gcgttctggt agttatctgc tgcgttaacc aggccgttct
    12721 ggctgagtga gaactgctgc ggcacgggca ttgccggacg ctgataatcc ggtgccagtg
    12781 aacaaccggt tagcgcaagg gccacacaaa atggcagaag tttacaagga gacataggct
    12841 cataatttct ggtgatttta tgctgccaac tttactcgcc aggctctgat tttccggtga
    12901 caggaaaatg acaaaattgt cattttgcca ataagcgatt gccatctgat cccgctactc
    12961 tagaattgcc cgggcaacat gcggaggaaa tatgaaactg ttgattgtcg aagatgaaaa
    13021 gaaaaccgga gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc
    13081 cgacaacggg ctgaatggct accatctggc gatgaccggt gattatgatc tgataatcct
    13141 cgatattatg ctgccggacg tgaacggctg ggatatcgtg cgcatgttgc gttccgccaa
    13201 taaggggatg ccgattctgt tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg
    13261 gctggagttg ggggcggatg actatctggt gaagccgttc gcttttgctg aactgctggc
    13321 gcgggtgcgc actctactgc ggcgcggagc ggcggtaatt atcgaaagtc agtttcaggt
    13381 tgccgatctg atggtcgatc tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac
    13441 tctgaccagt aaagaattta ctctgctgga gtttttcctc cgccatcagg gcgaagtgct
    13501 gccccgctcg cttatcgcct cgcaggtatg ggacatgaat tttgacagcg ataccaacgc
    13561 cattgatgtg gcggtgaagc ggctgcgcgg caaaatcgac aacgactttg agccaaagtt
    13621 gattcagacc gtgcgcggcg tgggttacat gctagaggtg ccggatggtc agtaagccat
    13681 ttcagcgccc gttttcgctg gcaacccgcc tgaccttttt tatcagcctc gccaccatcg
    13741 cggcgttttt cgcctttgca tggatcatga tccactcggt aaaagtgcat tttgccgagc
    13801 aggatattaa tgatttaaag gagattagcg ccacccttga acgggtactt aatcaccctg
    13861 acgaaacgca agcccgacgc ttaatgacgc tggaagatat cgtcagtggt tattccaatg
    13921 tgttgatctc cctggcaaat agccacggta aaacggtgta tcactccccc ggtgcgccgg
    13981 atatccgcga gtttacgcgt gacgccatac ccgataaaga cgcgcaggct ggcgaggtgt
    14041 atctcctttc cggcccgacg atgatgatgc cagggcacgg tcacgggcat atggaacaca
    14101 gcaactggcg gatgattaac ttgccggttg gcccgctggt tgacggcaaa ccgatttata
    14161 cgctctacat cgcgctttcg atcgattttc atcttcatta catcaatgat ttgatgaata
    14221 aacttattat gaccgcatcg gtaatcagca tcctgatcgt ctttatcgtg ctgttggcgg
    14281 tacataaagg tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcacctcga
    14341 aagatctcga cgttcgcctt gacccgcaga ccgtgcccat tgagctggaa cagctggtac
    14401 tgtcgttcaa ccatatgatc gagcgtattg aggatgtctt tacccgacag tccaatttct
    14461 cagcggatat cgcccacgaa attcgcacac ctattactaa tctcattacg caaacggaaa
    14521 tcgccctcag ccagtcgcgc agccagaagg agctggaaga tgtgctctac tctaatctcg
    14581 aagaactgac gcgaatggcg aaaatggtca gcgatatgct gtttctcgct caggccgata
    14641 acaaccagct aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt
    14701 tcgatttttt cgaggcgtta gcagaagatc gcggcgtgga gctgcggttt gttggcgacg
    14761 agtgtcaggt tgcgggcgat ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta
    14821 atgccctgcg ttatacgcca accggagaga caattgttgt gcgctgccag acggtcgatc
    14881 acctggtgca agttaccgtc gaaaaccccg gtacgcccat tgcgcccgaa cacttaccgc
    14941 gattgtttga ccgtttctac cgcgttgatc cttcccgcca gcgaaaaggc gaaggtagcg
    15001 gcattggcct ggcgatagtg aaatcgattg ttgtcgcgca taaaggcaca gttgcggtaa
    15061 cgtcagatgc tcgggggaca aggtttgtta tcatattacc cgcttaatcc ttcagcaaac
    15121 ggcaactttt ataaccagtg taaaaataac atgccgcaat agtaatacct attaatgata
    15181 aaaagctgtc acaattcata aaaaacctta atatacgcca ccctaaacat aaccagcgtt
    15241 aatgtaaggt ttttgtgtgg actggcttct tgatgttttt gctacctggc tctacggctt
    15301 aaaagtaatc gcgataacgt tagcggtcat tatgttcatc agcgggctgg acgatttttt
    15361 tattgatgtc gtctactggg tacgccgcat taaacgcaag ttgagtgttt atcgccgcta
    15421 cccgcgaatg agttaccgcg aactgtataa accagatgaa aaaccgttag cgattatggt
    15481 tccggcgtgg aatgaaacgg gcgtcatcgg caatatggcc gagctggcgg cgaccacgct
    15541 cgactacgaa aactatcata tctttgttgg cacctacccc aacgaccccg atactcagcg
    15601 tgatgttgac gaagtgtgcg ctcgcttccc gaacgtgcat aaggtagtct gcgcgcgtcc
    15661 tggccccacc agcaaagccg actgtctgaa caacgtgctg gacgccatca cccaatttga
    15721 acgtagcgcc aatttcgctt ttgctggttt tattctgcat gacgccgaag atgtgatttc
    15781 accaatggaa ttgcgtctgt tcaactatct ggtcgagcgt aaagatctga ttcagatccc
    15841 agtgtatccg ttcgaacgcg aatggacgca cttcaccagc atgacctaca ttgatgagtt
    15901 ttcagaactg catggcaaag atgttccggt gcgtgaagcc ctcgccggac aggtgcccag
    15961 cgcaggagtc ggcacctgtt tcagccgccg cgccgtgacc gcactgttag ctgacggtga
    16021 cggtattgct ttcgacgtgc agagtctgac tgaagattac gacattggct tccgcctgaa
    16081 agagaaaggt atgacggaaa tttttgtccg ttttccggtg gtggacgaag ccaaagaacg
    16141 cgagcagcgt aaatttttac agcatgcgcg gacgtcaaac atgatctgtg tgcgcgaata
    16201 tttccccgat accttttcga ctgcggttcg gcaaaaatct cgctggatca tcggcattgt
    16261 tttccagggc tttaaaaccc acaaatggac ctccagcctg acgctgaact actttctctg
    16321 gcgcgaccgc aaaggggcaa tcagtaactt tgtcagcttc ctcgcgatgc tggtgatgat
    16381 ccagcttttg ctgttgctgg cgtatgaaag tttgtggccc gatgcctggc atttcctttc
    16441 tattttcagc ggcagcgcat ggttaatgac tctgctgtgg ctaaactttg gcttgatgat
    16501 taaccgcatc gtgcagcggg tgattttcgt tactggctac tacggcctga cgcaggggct
    16561 gctatctgtc ctgcgtcttt tctggggcaa cctgattaac ttcatggcca actggcgcgc
    16621 gttaaaacag gtacttcaac acggcgatcc acgtcgtgtc gcgtgggata aaacaacgca
    16681 tgacttcccc agcgtgactg gcgatacccg ctcgttgcgt ccgttaggtc aaatcctgct
    16741 ggaaaatcag gtcatcactg aagaacaact cgatacagca ctgcgtaatc gcgtcgaagg
    16801 tctacgcctg ggcggttcaa tgctgatgca ggggctgatt agcgccgagc aactggcaca
    16861 ggcgctggca gagcaaaacg gcgtggcgtg ggaatccatc gatgcctggc agatcccttc
    16921 ctcgctgatt gccgaaatgc cggcctccgt ggcgttgcat tatgcggtac tgccgctgcg
    16981 tctggacaat gatgagttaa ttgtcggcag tgaagatggt attgacccgg tttcgctggc
    17041 ggccctgacg cgtaaagtcg gacgcaaagt gcgttacgtc attgttctgc gaggacaaat
    17101 tgtcaccgga ttacgccact ggtatgcacg ccgacgcggt cacgatccgc gggcaatgtt
    17161 gtacaatgca gttcagcatc agtggctcac ggaacagcag gccggtgaaa tctggcggca
    17221 atatgtgccg catcagttcc tgttcgcaga aatactgacc acgctcggtc atattaatcg
    17281 ttcagcaatt aacgtgttgt tattgcgcca tgaacgcagt tctctgccgc tcggcaagtt
    17341 tttggtcacc gaaggcgtta ttagccagga aacgttggat cgcgtcctga caattcaacg
    17401 cgaattacaa gtttcgatgc aatcactatt actcaaagca ggtttaaaca ctgaacaggt
    17461 tgcacaactg gagtccgaaa atgaaggaga ataaccttaa tcgcgtcatc ggatggtctg
    17521 gtttactgct gacatcttta ttgagtacca gcgcactcgc agacaatatc ggcaccagcg
    17581 cagaagagct ggggctgagc gattatcgcc attttgttat ttatccccgt ctcgataagg
    17641 cgctgaaggc acagaaaaat aacgacgaag caaccgccat ccgcgaattt gaatatatac
    17701 accagcaggt gccggataat attccgctga ctttatacct tgcggaagcc tatcgccatt
    17761 ttggtcatga tgaccgggcg cggctgttgc ttgaggatca actgaaacgt caccctggag
    17821 atgaccggct tgagcgcagt ctggcggcta ttccagttga agtgaaaagc gttacgactg
    17881 ttgaagaact gcttgcccag caaaaagcgt gcgatgctgc gccgaccctg cgttgtcgca
    17941 gtgaagtcgg gcagaatgcc ctgcggctgg cacaattacc tgtcgccaga gcgcaactta
    18001 acgatgcgac gtttgctgca tcgccggaag gaaaaacgtt gcgaaccgat ctgctgcaac
    18061 gggcaatcta cctgaaacaa tggtcccagg cagatacgct atataatgaa gcacgccagc
    18121 agaacacatt aagcgcggca gaacgccgtc agtggtttga cgtgcttctt gccgcgcagc
    18181 tggatgatcg gatcctggcg ctgcaatcac aggggatctt caccgatcct cagtcatata
    18241 ttacttatgc gaccgcgctg gcttatcgtg gcgaaaaagc acgcctccag cattatctca
    18301 ttgaaaataa gccgctgttt accacggacg cacaagagaa aagttggctc tatctgttat
    18361 ctaaatacag cgccaacccc gttcaggcgt tggcgaatta tacggtgcag tttgccgata
    18421 accgccagta tgttgttggc gcgacgctac cagtgctgtt aaaagaaggt cagtacgacg
    18481 cagcgcaaaa actgctcgcc accctccccg ccaatgaaat gcttgaggag cgttatgccg
    18541 ttagcgtagc aacccgtaac aaggctgaag ctctgcgtct ggcacgattg ctgtatcagc
    18601 aagaaccggc aagtcttacc cgtctggatc aactgacctg gcaactgatg cagaacgggc
    18661 agtcacgcga agctgccgat ttattgctgc aacgctatcc ttttcagggc gatgcgcgtg
    18721 tcagccagac gttaatggcg cgactggcgt ctctgctgga aagtcatcct tacctggcaa
    18781 cgccggcgaa ggtggcaatt ttatcgaaac ctttaccgct ggcggagcaa cgtcagtggc
    18841 aaagtcagtt gccgggtatt gctgataatt gcccggcaat agttcgcttg ctgggcgata
    18901 tgtcgccttc ttacgatgct gccgcctgga accgtctggc aaagtgttat cgggacacgc
    18961 tacccggtgt ggcgttgtat gcatggcttc aggccgaaca acgacaaccg gacgcctggc
    19021 aacatcgtgc ggtagcctat caggcgtatc aggttgagga ctatgccacc gcactgacag
    19081 cctggcagaa aatcagtctt cacgacatga gcaatgagga tctgcttgct gccgccaata
    19141 ccgcccaggc ggcaggaaat ggcgcggctc gcgatcgctg gttacaacag gcagaacaac
    19201 gtggactggg aaacaatgcc ctctactggt ggctgcatgc gcaacgttac attccaggtc
    19261 agccggaact cgcactgaac gatctcacgc gctcaatcaa tattgcgcct tctgccaacg
    19321 cttacgttgc gcgggcaaca atttatcgcc aacgtcataa tatcccggcg gcggtgagtg
    19381 atttgcgcgc cgcgctggaa ctggaaccga ataatagcaa cacccaggca gcgcttggtt
    19441 acgccttgtg ggatagcggt gatatcgcac agtcgcggga aatgctcgaa caggcgcata
    19501 aagggctacc ggacgatccg gcacttatcc gacaactggc ctacgtgaac caacgtctgg
    19561 atgacatgcc tgcgacgcag cactacgccc gactggtgat tgatgacatt gataatcagg
    19621 cgctgataac cccactgacc ccagagcaaa atcagcaacg cttcaatttc cgccgtctgc
    19681 atgaggaggt cggtcgccgc tggtcgttca gtttcgattc ttctatcggc ttgcgttccg
    19741 gcgcaatgag taccgctaac aataatgtcg gcggcgcagc gccagggaaa agctatcgta
    19801 gctacgggca actggaagcc gagtaccgcc tcggacgcaa tatgctgctg gaaggcgacc
    19861 tgctctcagt ttatagccgc gtctttgccg ataccggtga aaacggggtg atgatgccgg
    19921 tgaaaaatcc gatgtccggc accggtctgc gctggaagcc actgcgcgat cagatctttt
    19981 tcctcgccgt cgaacagcag ttgccgctga acggacaaaa tggcgcatcc gataccatgc
    20041 tgcgcgccag cgcctcattc tttaatggcg gcaaatacag cgacgaatgg cacccgaacg
    20101 gttcaggctg gtttgcccaa aacctgtacc tcgatgcggc gcaatatatc cgccaggata
    20161 ttcaggcgtg gacggcagat tatcgcgtca gctggcatca gaaggtggcg aacgggcaga
    20221 ctattgagcc ttatgctcac gttcaggaca acggctatcg tgataaaggc actcagggcg
    20281 cgcagcttgg cggtgtcggg gtccgctgga atatctggac cggcgagacg cactacgacg
    20341 cctggccgca caaagtcagt ctcggcgtcg aatatcaaca tacctttaag gcgattaatc
    20401 aacgtaacgg agagcgcaac aacgcgtttc tcaccattgg agtgcactgg taaatgcgta
    20461 agttcatttt cgtattgctg acactgcttt tggtcagccc tttttccttt gcgatgaaag
    20521 gtattatctg gcaaccacaa aaccgtgata gtcaggttac cgatacccag tggcaggggc
    20581 tgatgagtca gttacgtttg caaggcttcg atacactggt tttgcaatgg acccgttacg
    20641 gcgatgcatt cacccagcca gaacagcgcg cgttattgtt taagcgtgct acagctgcgc
    20701 aacaggctgg cctgaagctg attgtcgggc tgaacgccga tccggaattt tttatgcacc
    20761 agaaacagtc gtccgcagcg ctggaaagct atcttaatcg cctgctggct gccgatctcc
    20821 agcaagccag attatggagc gccgtgcctg gcgtaacgcc ggatggctgg tacatcagcg
    20881 cggaaattga cgacctgaac tggcgcagcg aagccgcccg tcagcctttg ctaacatggt
    20941 taaacaacgc gcagcggttg attagcgatg tttcagcaaa accggtttat atcagtattt
    21001 ttttcgccgg aaacatgtcg ccagatggct atcgccaact gctggaacaa gttaaagcaa
    21061 ccggtgttaa tgtctgggta caggatggca gcggcgtgga taaactgacc gctgaacagc
    21121 gtgaacgtta tctacaggcc agcgccgatt gccaaagttc cgccccagca agcggcattg
    21181 tttatgaact gtttgtcgct ggtaaaggta aaacgtttac agcgaaaccg aaaccggatg
    21241 cagagatcgc ctcgctgtta gcgaaacgtt cctcttgcgg taaagacacg ctctatttct
    21301 ctctgcgcta tttgcccgtc gcgcagagta tccttgagta ttaatgatta aagggagcaa
    21361 cttagcttgc tcctttcctt ttcactttcc taatcaaaat cctggcaaga tgaattattt
    21421 agcagaatat ctgttttcat tttattcgtc tgacgtgttc caattattta gcaaaaccgc
    21481 gacaacaaag tgatggtgta atttgtaaaa gggaacaaat aaatggctcg ccctgcagga
    21541 ttcgaacctg tagcccacgg cttagaaact ccaggaacga cattttaagt caacaaatta
    21601 ccgcgccatc tctgcgttca cacgtcccac tacctcaaaa tatgtaaaac cttgcaagcc
    21661 attgcgaggc cttatgtgtc tcagttttgt tcctcttttt tatactaaaa aacacagcaa
    21721 ttgaggataa acctcatgct attatcgcta atatgcatct aaaggcatgg cacttgaata
    21781 gataaaaact ccacaaagaa aaaaaataat t
//
LOCUS       DAOFSV010000066        21725 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.066, whole genome shotgun sequence.
ACCESSION   DAOFSV010000066 DAOFSV010000000
VERSION     DAOFSV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.066"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..238)
                     /gene="bhsA"
                     /locus_tag="QBH27_002656"
     CDS             complement(<1..238)
                     /gene="bhsA"
                     /locus_tag="QBH27_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDQ7014951.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHG"
     gene            479..1111
                     /gene="comR"
                     /locus_tag="QBH27_002657"
     CDS             479..1111
                     /gene="comR"
                     /locus_tag="QBH27_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDQ7014952.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDHYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(1173..1712)
                     /locus_tag="QBH27_002658"
     CDS             complement(1173..1712)
                     /locus_tag="QBH27_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDQ7014953.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1939..3243)
                     /gene="ndh"
                     /locus_tag="QBH27_002659"
     CDS             complement(1939..3243)
                     /gene="ndh"
                     /locus_tag="QBH27_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDQ7014954.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3642..4184)
                     /locus_tag="QBH27_002660"
     CDS             complement(3642..4184)
                     /locus_tag="QBH27_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7014955.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(4207..5232)
                     /gene="nagZ"
                     /locus_tag="QBH27_002661"
     CDS             complement(4207..5232)
                     /gene="nagZ"
                     /locus_tag="QBH27_002661"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDQ7014956.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(5243..6067)
                     /gene="thiK"
                     /locus_tag="QBH27_002662"
     CDS             complement(5243..6067)
                     /gene="thiK"
                     /locus_tag="QBH27_002662"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDQ7014957.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(6048..6689)
                     /gene="lpoB"
                     /locus_tag="QBH27_002663"
     CDS             complement(6048..6689)
                     /gene="lpoB"
                     /locus_tag="QBH27_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDQ7014958.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPTEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6703..7080)
                     /locus_tag="QBH27_002664"
     CDS             complement(6703..7080)
                     /locus_tag="QBH27_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDQ7014959.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(7083..7442)
                     /gene="hinT"
                     /locus_tag="QBH27_002665"
     CDS             complement(7083..7442)
                     /gene="hinT"
                     /locus_tag="QBH27_002665"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDQ7014960.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7776..9965
                     /gene="fhuE"
                     /locus_tag="QBH27_002666"
     CDS             7776..9965
                     /gene="fhuE"
                     /locus_tag="QBH27_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDQ7014961.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDRSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTKWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNLSITGTYQF"
     gene            complement(10025..11458)
                     /gene="ptsG"
                     /locus_tag="QBH27_002667"
     CDS             complement(10025..11458)
                     /gene="ptsG"
                     /locus_tag="QBH27_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDQ7014962.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLT
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11753..12550)
                     /locus_tag="QBH27_002668"
     CDS             complement(11753..12550)
                     /locus_tag="QBH27_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ7014963.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12561..13565)
                     /gene="holB"
                     /locus_tag="QBH27_002669"
     CDS             complement(12561..13565)
                     /gene="holB"
                     /locus_tag="QBH27_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDQ7014964.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYGVQSGDWYSLLAALNNEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13562..14203)
                     /locus_tag="QBH27_002670"
     CDS             complement(13562..14203)
                     /locus_tag="QBH27_002670"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDQ7014965.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(14193..15215)
                     /gene="yceG"
                     /locus_tag="QBH27_002671"
     CDS             complement(14193..15215)
                     /gene="yceG"
                     /locus_tag="QBH27_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDQ7014966.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(15218..16027)
                     /gene="pabC"
                     /locus_tag="QBH27_002672"
     CDS             complement(15218..16027)
                     /gene="pabC"
                     /locus_tag="QBH27_002672"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDQ7014967.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(16147..17388)
                     /gene="fabF"
                     /locus_tag="QBH27_002673"
     CDS             complement(16147..17388)
                     /gene="fabF"
                     /locus_tag="QBH27_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDQ7014968.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17476..17712)
                     /gene="acpP"
                     /locus_tag="QBH27_002674"
     CDS             complement(17476..17712)
                     /gene="acpP"
                     /locus_tag="QBH27_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDQ7014969.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17923..18657)
                     /gene="fabG"
                     /locus_tag="QBH27_002675"
     CDS             complement(17923..18657)
                     /gene="fabG"
                     /locus_tag="QBH27_002675"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDQ7014970.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18670..19599)
                     /gene="fabD"
                     /locus_tag="QBH27_002676"
     CDS             complement(18670..19599)
                     /gene="fabD"
                     /locus_tag="QBH27_002676"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDQ7014971.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19615..20568)
                     /gene="fabH"
                     /locus_tag="QBH27_002677"
     CDS             complement(19615..20568)
                     /gene="fabH"
                     /locus_tag="QBH27_002677"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDQ7014972.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20636..21706)
                     /gene="plsX"
                     /locus_tag="QBH27_002678"
     CDS             complement(20636..21706)
                     /gene="plsX"
                     /locus_tag="QBH27_002678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDQ7014973.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
BASE COUNT         5376 a         5889 c         5232 g         5228 t
ORIGIN      
        1 ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt gcgcccatct
       61 catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc gcgttagcac
      121 tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca gccgcaaagc
      181 tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg tttttcataa
      241 tagtggcctt atgcagatga atgacgtcgc aagcaattac ttaacgatcg ataactaaat
      301 gatagatgtg atctggatca catacaagat attttttata acaatcatta attaatttaa
      361 aaacctttaa atttcagtta aatagaaagc gattattttt atggtttctg gactggtgaa
      421 cggaggtgat tatttttata ataacctttc attcacagtc accaggtaca acggcatcat
      481 ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag
      541 ggatgccgcg cttgataagg ccatgaaatt gttctggcag cacggttatg aagcgacttc
      601 tcttgcggac ctcgttgaag cgaccggagc taaagcgccc acgctatacg cggaatttac
      661 caacaaagag gggttattcc gcgccgtact cgaccactat atcgatcgtt ttgccgccaa
      721 gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt
      781 tgctgccatc gccaactgct ttaccagcaa agacactccg gctggctgct tcatgatcaa
      841 caactgcacc accctctccc cggattcagg agatatcgcc aatacgctga aatcacgcca
      901 tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat
      961 cccgacccac tgtgacgtga cgcatctggc agaattcctt aattgtatta ttcaggggat
     1021 gtcgatcagc gcacgcgaag gtgcatcact ggaaaaactg atgcagattg cccgaacgac
     1081 tttgcgttta tggccggaac tgctgaaata ggtcgcaaaa aaagcccctc agcctgagcg
     1141 aatgaggggc taaattgcag agtacagcct tgttatactt tgttattcaa aatcagctgc
     1201 ccgttgctat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc
     1261 tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc
     1321 gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc
     1381 tggttacctg catatccacc ccccagcgcc cccacaacag tggcgacatc tttaccgcga
     1441 ccacctccaa actgatgccc aatcacgccg ccagcaacag cgccgagcac cgacccggta
     1501 atgcgatttt catcctgcac cggtcgacga tgggtcactg taacgttgcg acactcctga
     1561 cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg
     1621 ccacgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca
     1681 atcccgatac ccgccaacat tgacttattc acgggaacac ctccttcttt gtagttatgc
     1741 caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg
     1801 gctggaaata ctcagggatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc
     1861 gtcttatcag gcctaccgct cgtaccattt gtccagggcg gataaggcgt ttatgcccca
     1921 tccgccagta caagtcgatt aatgcaactt caaacgtgga cggataacgc ggttaatact
     1981 ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct ggtgcattcg
     2041 gtacagcgag atgtatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt
     2101 caggttaccc atcaggctac cgactgtgga aaagttcgac agcgatacca gcgaaccgtg
     2161 atctttatac tgataatttt tcagcggctt accgttcatc tgcgccagaa tgttgttcat
     2221 tgcgcaggtt gccatctgat gtgcggcctg agcgcgcggc ggaacaaagc ccccttccgg
     2281 acgcgggcat gacgcgcagt cgccaatagc gtatatgtct ggatcgcgag tggtttgcag
     2341 cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga tatctttcag
     2401 gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcgcc
     2461 atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg
     2521 aacgccaagt ttcgttaact cactatgggc cgcagcagag atacgtggcg gcaatgcagg
     2581 caaaatacgt tcgcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcagaccttt
     2641 ataaccgtaa ctgtgcagtt gcttgaccgc gttgtgcaat tcagcggaga gttctacacc
     2701 cgtcgcgccg ccgccgacaa tcgcaatgtt tactttgcca ttcgcgccca ggttggcgga
     2761 gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc
     2821 gaggaaaatg cagttctctt tgacacctgg tgtattgaaa tcgttagagg tgctacccag
     2881 cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc
     2941 gtcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa ttacggaacc
     3001 cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc
     3061 ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacaagt ggctgtggtt
     3121 acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt
     3181 tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt
     3241 caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttatta acatagctct
     3301 taattaacaa caggttatag caatgaaaat gttcttcgct attgagaata acatgaatgg
     3361 tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact
     3421 ttaccgggtg agatcaaaaa acagagtttg ttgttcatta tttgagctga tttgtgagaa
     3481 tgatatagag tgaggtgtgt atggagggcg ttcggtaggc ctgataagag gcgcatcagg
     3541 cattgatgct cgactgtcgg atgcggcgga acgccttatc caacccacag aatcaggagc
     3601 gcatcaggta ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac
     3661 gctttaatgc gctgtaaatg cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc
     3721 cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt aagcgcttca
     3781 tcattacgcg acaaaatcac caggcaacga tcgcgattct tctcacggaa gttggtcaca
     3841 cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttcccttc catgttctca
     3901 taagggaaca aattggggtt gaagatcacc tggcggatat cacagagaaa accaatccgc
     3961 tcagcccaat aaccgcccag accaacacca caaatgagcg ggcgctcgtc gacgttcagt
     4021 tgcaacattt tgtccacttc tttgagcaaa tgctgcatat catgttttgg atgccgcgta
     4081 ctgtagctta ttagccgtac atccgggtca ataaactgca attgtaagac tttctcgtgg
     4141 ttacccggac tgttagagtc aaaaccgtgc aaatagataa tcatcgcttc ctcacataag
     4201 ccagggttaa tgacctgcct tctcttcctg ccagcgttca tgtaactgat tcagacgggc
     4261 gctgatcgct ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg
     4321 atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc
     4381 ccctttacga ttattgcaga ccaggatcat atcgcaacct gcatccagtg atgcctgtcc
     4441 acgctcggca taactgccca taatcgctgc gccttccatc gataaatcgt cagagaaaat
     4501 cacgccgtca aaacccaatt cctgacgcaa aacggttttc agccagtagg gagaaccgct
     4561 cgccggacgc ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt
     4621 attttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgcgg
     4681 acgtgggtcg cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt gacctgggaa
     4741 gtgtttcccg gtagttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat
     4801 tgccagggct ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg
     4861 cccgacgtcc agcactggcg caaagctgat atcaatatcc atagcaatca tttcgctggc
     4921 catcaaccaa cctgcctctt gcgccagttt tccgccttct tccattccca acagcgcagc
     4981 gaatgattgt gccgctggca agcgggtaaa accttcacga aaacgctgca cgcgtccacc
     5041 ttcctgatct accgccacca ccagatgatt tcgcgatgct gcgcggatct ggcgcaccag
     5101 ttcacgtaac tgggcaggat catgatagtt tcgcgtaaag agaatcagcc ctcccaccag
     5161 cggatgcgcc agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat
     5221 tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg
     5281 ccagcctgat aaattgttga tcgccggttt gtcgccagcg gtactcaaac caccctgctt
     5341 tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa
     5401 tcttcgcgcg agtggcatag tcattgacca attgccgatg ctggtcagta ttttccaccc
     5461 acaccgccgc cagttccagc gcgatatcac catctccggc atactcccag tcgatgagtt
     5521 ttaaccctga cgcgctatgc actaaatttc cggcgtggac atccatatgc aatggactta
     5581 agcgtaaaag ccgtggttcc cgcgctttgc gcagacgttt taacattcgc agccaaccca
     5641 ctgtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaacggc aacagcgtta
     5701 ttcgccagcc aaaacgcggt tgttgatgta gataatacag caagcctgcc agttcgttgg
     5761 tatccggcaa atacgttttt acctcgccgg gcagatagtc aaccaccatc cagtcacgga
     5821 gatataaatg cggcttcggt gcaatgcatg cgggtagttg tgataaagcc cgatactggc
     5881 gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg acgcacaaca aaacgctggc
     5941 cctgatgttc aatgagaaaa ctcccgccac taagcccact attaaacgtc gtgacgggat
     6001 gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gaggggatta ttgctgcgaa
     6061 acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt
     6121 agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccaaca
     6181 ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct
     6241 aattgttgct tagccatcga cagctgctgg gcggaaacca gggtaaattt accgttatta
     6301 gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcaacgaacc gttagtacgg
     6361 ttgttaacgc tatcaaccag caggacgcta cccgcagtca cgccatcagc cccaagcatc
     6421 ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc
     6481 ggtgcagttt gatcttcgtg ctcaattggg cctggctgct gcggtatcgt cggcaccgat
     6541 ggcactgtgg ggacaggctg ttgtggctcg gttggttgtt ccggcgctgg tttcacttct
     6601 tctaccggtg caggttcacg ttgccccaca cacccggcaa gaaacatcgc cagcgcggta
     6661 atcaaggcgt agcgactcat ttttgtcatc aagattcacc ccttacaaat atagataaag
     6721 tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgtgaccg ccgaatgtgc
     6781 gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata
     6841 ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga
     6901 tgctgaaggt tgaatatcag atgtcgaaag gacgggcttt tctgcactga tgcccgcagc
     6961 caataaagat gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga
     7021 acgacagccc accagcacta acaacgccag agacaccagc ccaaagcatc cttttctcat
     7081 cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata
     7141 tggatgtggt aaacctcttg tccgccatgg cggttggtgt tcatgatcag acgatagcca
     7201 tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc
     7261 gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc
     7321 agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg
     7381 tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc
     7441 acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc
     7501 gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag
     7561 caaaaaaccg ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg
     7621 ctgaaaagac attttctgtt tggtttacac accaaatatg aatgcgtata tttctcattt
     7681 gcatttacat acaaaattat tcgcacacta aatataaccg ctcatcaccc tgctccggca
     7741 tgagcctgac gccaacataa accaagagat ttcaaatgct ttcgacacaa tttaacaggg
     7801 ataatcaaca tcaagccatc atcaaaccgt cactccttgc cggttgcata gcactggcac
     7861 tattaccttc cgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt
     7921 cagccacagc cccagatgat ggcgaaaatg attacaacgt aacgtctacc tctgcgggta
     7981 caaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc
     8041 ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca
     8101 gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata
     8161 actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac
     8221 tttctgatat ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggcctcatga
     8281 ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg
     8341 aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg
     8401 atttacaaag cccactcacc gaagacggta aaatccgcgc acgaattgtc ggcggctacc
     8461 agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc tccggcattg
     8521 tcgatgctga tttaggcgat cttacgatgc tttcagccgg ttacgaatat cagcgtattg
     8581 atgttaatag cccaacctgg ggcggtttac cgcgctggaa tactgatggc agcagcaaca
     8641 gttacgatcg cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca
     8701 aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaagcgacg ctgaatgcca
     8761 cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag
     8821 cggatggtat gctggttggg ccatacagta attatggccc tggttttgat tatgtcggag
     8881 gcaccggttg gaacagcggc aaacgtaaag ttgatgcgct ggatctgttc gctgacggta
     8941 gttatgaatt gtttggtcgt cagcacaatt taatgtttgg tggcagttac agcaaacaaa
     9001 acaatcgtta cttcagttca tgggccaaca ttttcccgga tgaaattggc agtttctaca
     9061 actttaatgg caatttccca caaaccgaca ggtcaccaca gagtctggcg caggacgata
     9121 ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc
     9181 tgatcctcgg cgcacgttat accaagtggc gggttgatac gctgacttac agcatggaga
     9241 aaaaccacac cacgccttat gctggactgg tgtttgacat caatgataac tggtcgacct
     9301 acgccagcta tacctctatt tttcagccgc aaaatgatcg tgacagttca ggcaaatatc
     9361 tggcaccaat caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc
     9421 gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg
     9481 gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca
     9541 gtaaaggtgt ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacgtttg
     9601 gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct aatctgccac
     9661 gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag ttgacggtcg
     9721 gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct
     9781 tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga
     9841 aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac gataccaacg
     9901 tggaaggttc tattgtctac ggcgcaccgc gtaatttaag cattaccggc acgtatcaat
     9961 tctgaataac gcctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta
    10021 cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac
    10081 cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca
    10141 ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg
    10201 cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt
    10261 cgctggtacc tgtcgctttt gcgtcttcag tcgcgtcttc acgacccggc gttttcagat
    10321 ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatggcataa ccgataccga
    10381 cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac
    10441 cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg aatgccaggc
    10501 ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag gagaactcga
    10561 tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca
    10621 ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac
    10681 cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat
    10741 aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca
    10801 tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa cgttcgatga
    10861 aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct
    10921 ggattgcaga gccaataggc ggccaaatga aggacagcac aacgccagta aagatggcag
    10981 tcaggcctga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca
    11041 gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag ataatccccc
    11101 cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt aaatgcagta
    11161 ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca
    11221 gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg
    11281 gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca
    11341 gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggc agtacggata
    11401 ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa
    11461 ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc aaggggggag
    11521 tgcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt
    11581 cagtgccgat ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac
    11641 attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc cgattttcgg
    11701 cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga
    11761 tggattgaag gcgggaagca tcgatgtgaa acagacgggc gaagttatcg gtggttacct
    11821 gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa
    11881 ccatcgcagg ttgattctct tttcctcgat gcggtactgg cgcaaggtaa ggtgagtcag
    11941 tttccaccag taaccgatcc agggggacat aacgcgcagc atcgcgcagt tgctccgcat
    12001 tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg
    12061 ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt
    12121 cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt gtggacgata accggcttgt
    12181 tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt
    12241 ccggcgtgta ataataatcc agcccggttt cacccagcgc tacaacgccc tcttctgccg
    12301 ccagacgacg taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac
    12361 aagaaaatac gacgttgtcg cgttcgccta ccagatcccg catgtgtaaa taacccggta
    12421 atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt
    12481 catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga
    12541 ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg
    12601 taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat acctgtgaca
    12661 gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga
    12721 gaaagatggt ttgccagttc gacgaccagc cccggcacat caacattggt cacgtgcgca
    12781 gcgccataat ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgcgcc
    12841 ggagcttgtt cattattaag ggccgctagc agcgaatacc agtcgcccga ttgcacgcca
    12901 tatgccagcg cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc
    12961 agtgccgccc caggcgaacc ggcgcttaag cgcaatgcgg caagtagtgc atcctgtgac
    13021 attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa
    13081 tgtaaccgac aacgactacg taatgttgcc agtaaacgtt ctggctcgcg ggtagcaagg
    13141 aaaaaccacg tctctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg
    13201 tcggttagta aggcagcatc ggttacccag acaactttcg caccacctaa gcgtgcgtgc
    13261 tcattcagct tttcggtgac ctcacgtacc gcatcaatgc ccagcgcatt tttccctttt
    13321 tcgggagcca gggtgtagta atcgggatgt gtgccagcct gcatcaactg acatccacga
    13381 cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg
    13441 tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct
    13501 cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat
    13561 ctcatgcgtc caactccttc acccaattgg tcacggtagt gcggattgca tccatcacgg
    13621 cctccagcgg ctgggtggca tcaatggtat gaatgctttt atcttgtgct gccagttcca
    13681 gatagcgggc gcgggtgcga ttaaagaaat cgaatgactc ttgctcaata cgatccagct
    13741 caccacgggc gcgagcacgt tttaagccga cctctggggt gacatcaaga taaagcgtta
    13801 agtcagggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa
    13861 taccacgtcc tccaccctga taggcttgag tggagagatc gtggcgatcg ccaatcaccc
    13921 aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat
    13981 aaaacatcaa aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca
    14041 gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca
    14101 tgtcgcggat acccagttgc tcaagcgtct caaccaccac gttacgcgcg gtagttttgc
    14161 ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc cttaagcact
    14221 ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca
    14281 cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc
    14341 agcgaatccg cccccggcgt cgctatcgca cctggcggca gaccggtaat ggtataggtg
    14401 ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc
    14461 atcccgtaaa tcacggtcgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata
    14521 aatactgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat
    14581 gccatcgtca ccaactggtt tttatcttta taaggcagac cgtccgcacg cccttcccag
    14641 gcgctatcga ccgctttcac cattttcttg tgcgctcgtt tgagtaacgc gacatcggtg
    14701 gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt
    14761 tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cgctcagcgt gtgcttgata
    14821 tacggagcct cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc
    14881 agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc
    14941 tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc
    15001 agccattgga aaacccgtgg gcgattgatg atcttatcgg cataaagctg ttcaccgagc
    15061 gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg
    15121 ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc
    15181 agcaataaca agattatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg
    15241 gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgtacgg
    15301 gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcttcca
    15361 gagaggcttg cacttcgaca agctgataag aggattgtgc cagcaaacgg atacagaatt
    15421 gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acgttgccct
    15481 tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca
    15541 gcgcctcatc agcgtttgtc tgctcaagat gagagcggat caatacttgc tcaagccgat
    15601 taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca
    15661 acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta acggagagaa
    15721 tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga
    15781 tcacgacttt cagtacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa
    15841 gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga
    15901 tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat
    15961 caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttatga ccgttaatta
    16021 agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca
    16081 agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttataag ggtggaaaat
    16141 gacaatttag atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggaatt
    16201 acacagagtg tattccattc cgctaacctg acgcgcttcg tgcggtacga aatccaaatc
    16261 gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc
    16321 aaggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga
    16381 tttcgtggag cttaccaaca cgcggcttgc agcttcaccg aagatggttt tcaccgcctg
    16441 cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg
    16501 actcgcttca atgcctgcat cacgcagagc atttgccatc gccagagctg cgcctgcgcc
    16561 attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc
    16621 agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc
    16681 ggcaccatcg cccagtacga aaccatcacg ctctttatcc cacgggcggc tcgccgcttg
    16741 cgggttatca ttgcgggtag ataatgcgcg tgccgcgcca aaaccaccaa cgcccagcgg
    16801 cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat
    16861 acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct
    16921 tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga
    16981 cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc
    17041 ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt
    17101 ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga caattccata
    17161 ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa
    17221 atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag
    17281 gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac
    17341 aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca
    17401 gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    17461 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    17521 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    17581 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    17641 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    17701 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    17761 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    17821 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    17881 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    17941 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    18001 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    18061 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    18121 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    18181 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    18241 cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc
    18301 ttttgacaga cggaaaacag atgaaaggtt ggtttcgata atatcgttcc actcttcatc
    18361 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    18421 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    18481 attcaacatc agacctttgc cgttggcacc taaataatca ctgatcgcct gagcgccatt
    18541 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    18601 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat
    18661 tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    18721 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc actttgcccg ggccgacttc
    18781 atagagatgt tctacgcctt gcgctgccat gtattcaaca gacttcgtcc actgaaccgg
    18841 gttatacaac tgacgtacca gtgcgtcacg aatggcatca ccattggttt cgcatttcac
    18901 atcaacgtta ttcacaacag gaactgttgg tgcgttaaag gtgattttcg ctaattctac
    18961 tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtag
    19021 cggcagagcg cgttttgcgc ccgcagcttt acaagcagca cccgcacgct caaccgcttc
    19081 tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    19141 accttctgca gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc
    19201 cgccatagca cccgttcctt ccggtacggc ttcttgcatg aacttgccgc gcatctcaac
    19261 cagacgcacc gcatcagcga aatcaatcac accagcgcaa accagcgcgg agtattcccc
    19321 caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata
    19381 cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    19441 agctggcccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc
    19501 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    19561 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    19621 acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg
    19681 ccccggctta atgcgcccgt cgcgtacagc ttcatccaga gcgcacggga cagaggccgc
    19741 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca tgccgagttt
    19801 tttcgctgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    19861 agaacggtca agattattcg ccgtcagcgt ctcatcaacg atgtgcgcca gttctgttac
    19921 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    19981 acggtcagca ttaggcagcg tcagcaactc accatagcta ccgtcggcat gcagatgggt
    20041 ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    20101 aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac
    20161 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    20221 ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccagcat
    20281 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    20341 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    20401 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    20461 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    20521 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    20581 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    20641 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    20701 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    20761 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    20821 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    20881 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    20941 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aatgtgacat
    21001 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    21061 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    21121 tactgtcgag accctttact tcttcttcac caatattgag caacgccacg cgaggattag
    21181 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    21241 tactgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat
    21301 gcggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    21361 ttgccagccc cattagcgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    21421 ctttcaccag ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg
    21481 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    21541 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    21601 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    21661 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    21721 ccggt
//
LOCUS       DAOFSV010000067        21723 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.067, whole genome shotgun sequence.
ACCESSION   DAOFSV010000067 DAOFSV010000000
VERSION     DAOFSV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.067"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(7..1425)
                     /gene="phrB"
                     /locus_tag="QBH27_002679"
     CDS             complement(7..1425)
                     /gene="phrB"
                     /locus_tag="QBH27_002679"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDQ7014974.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGAIDPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(1422..1931)
                     /locus_tag="QBH27_002680"
     CDS             complement(1422..1931)
                     /locus_tag="QBH27_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDQ7014975.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(2032..2238)
                     /locus_tag="QBH27_002681"
     CDS             complement(2032..2238)
                     /locus_tag="QBH27_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDQ7014976.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            2551..2640
                     /gene="kdpF"
                     /locus_tag="QBH27_002682"
     CDS             2551..2640
                     /gene="kdpF"
                     /locus_tag="QBH27_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDQ7014977.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            2640..4313
                     /gene="kdpA"
                     /locus_tag="QBH27_002683"
     CDS             2640..4313
                     /gene="kdpA"
                     /locus_tag="QBH27_002683"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDQ7014978.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            4336..6384
                     /gene="kdpB"
                     /locus_tag="QBH27_002684"
     CDS             4336..6384
                     /gene="kdpB"
                     /locus_tag="QBH27_002684"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDQ7014979.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            6393..6965
                     /gene="kdpC"
                     /locus_tag="QBH27_002685"
     CDS             6393..6965
                     /gene="kdpC"
                     /locus_tag="QBH27_002685"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDQ7014980.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPVLDKQIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNVVELNLALDKLDE"
     gene            6958..9642
                     /gene="kdpD"
                     /locus_tag="QBH27_002686"
     CDS             6958..9642
                     /gene="kdpD"
                     /locus_tag="QBH27_002686"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDQ7014981.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHIEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            9639..10316
                     /gene="kdpE"
                     /locus_tag="QBH27_002687"
     CDS             9639..10316
                     /gene="kdpE"
                     /locus_tag="QBH27_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDQ7014982.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            10591..10695
                     /locus_tag="QBH27_002688"
     CDS             10591..10695
                     /locus_tag="QBH27_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDQ7014983.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            11005..13203
                     /gene="speF"
                     /locus_tag="QBH27_002689"
     CDS             11005..13203
                     /gene="speF"
                     /locus_tag="QBH27_002689"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDQ7014984.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            13200..14519
                     /gene="potE"
                     /locus_tag="QBH27_002690"
     CDS             13200..14519
                     /gene="potE"
                     /locus_tag="QBH27_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDQ7014985.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(14576..16216)
                     /gene="pgm"
                     /locus_tag="QBH27_002691"
     CDS             complement(14576..16216)
                     /gene="pgm"
                     /locus_tag="QBH27_002691"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDQ7014986.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(16242..16787)
                     /gene="seqA"
                     /locus_tag="QBH27_002692"
     CDS             complement(16242..16787)
                     /gene="seqA"
                     /locus_tag="QBH27_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDQ7014987.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFTAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            16972..17736
                     /gene="ybfF"
                     /locus_tag="QBH27_002693"
     CDS             16972..17736
                     /gene="ybfF"
                     /locus_tag="QBH27_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ7014988.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            17876..18169
                     /gene="ybfE"
                     /locus_tag="QBH27_002694"
     CDS             17876..18169
                     /gene="ybfE"
                     /locus_tag="QBH27_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDQ7014989.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            18309..18839
                     /gene="fldA"
                     /locus_tag="QBH27_002695"
     CDS             18309..18839
                     /gene="fldA"
                     /locus_tag="QBH27_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDQ7014990.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            19049..19135
                     /gene="uof"
                     /locus_tag="QBH27_002696"
     CDS             19049..19135
                     /gene="uof"
                     /locus_tag="QBH27_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDQ7014991.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            19128..19574
                     /gene="fur"
                     /locus_tag="QBH27_002697"
     CDS             19128..19574
                     /gene="fur"
                     /locus_tag="QBH27_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDQ7014992.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(19658..19984)
                     /locus_tag="QBH27_002698"
     CDS             complement(19658..19984)
                     /locus_tag="QBH27_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7014993.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(20034..21440)
                     /gene="chiP"
                     /locus_tag="QBH27_002699"
     CDS             complement(20034..21440)
                     /gene="chiP"
                     /locus_tag="QBH27_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDQ7014994.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSANDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         5048 a         5548 c         5705 g         5422 t
ORIGIN      
        1 gttttatcag gctcctttcc tcgcttcttc gtacgctgcc agcgttcgca gccttgcctc
       61 tttgtgatcg actatcggtt gcggataatc caacgtcaca cctgttttct ctgcccactt
      121 ccacggctca tgcaccgctt tccccggtac atcgcgcagc tccggtagcc actggcggat
      181 aaattcgcct tcgcggtcaa atttctcgcc ctgagttgtc gggttgaaaa tacgaaaata
      241 cggcgctgca tcagtacccg tagaagcggc ccactgccag ccaccgttat tagctgccaa
      301 atcaccatca atcagctgcg acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa
      361 atctttcacc agaaaactgg ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct
      421 gttaagctga cgcatggcag catcgacaat cgggtatccc gttttgcctt cctgccaggc
      481 ctttaaatgg gcgggattac tttgccactg tacacgatcc gtccaggcaa taaacggacg
      541 atgtttacac agcgaaggat aatacgtcat caaatggcgg tagaactcgc gccagatcag
      601 ctcactaagc cagacactac cggctccacc gtccagcgcc tgcggctgtt ccgccaacaa
      661 gcgatgcaaa cactggcgag gcgataaccc gcccgttgcc aggctggcgg acaaacgact
      721 ggtgccttct actgccggaa aatctcgttg ttgctcatac tctccggcac cgttctggca
      781 aaactggcgt aattgcgcaa tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa
      841 agactgacga ggataattca gcgtaatgga tggcgcggga tctatcgctc cactactacg
      901 tacttttggc gcagcgacgc actccggcat cccttcccgc agccgtttca gccaggcatt
      961 cttaaaaggc gtaaagactt tgtacatctc atgattgcca gtcatcaccg cccccggcgg
     1021 caggatcacg ctgtcatcaa atccttcaca caccacgtta cgcagcgctc tttcaacttc
     1081 cacatcccgc gcccgctcat ttacctcata ctgatagtta taaaaaagat gagtaacgcg
     1141 gttttccgcg cacacctgtt taactatttc gacgctggcg gcaaagtcat ccacttcacg
     1201 gaacaataaa ggaatacctt tttccgccag cgcgttttgc agcgcattaa gttgtgtatt
     1261 gataagttca gcctgacgcg gcgacacatt atgcgccgcc cactggcgtg gggtagcgat
     1321 atacaacgcc agcacgcgtg cagacgaatt gcggcaggcg gcagccagtg cgagattatc
     1381 gtgcagacgt aaatcctggc gaaaccagac cagatgggta gtcataaaac tcctgcatgg
     1441 cgcaagttaa tcacacgcgg ccaaagttcg aaataacgct gccctgaaag ccaggcgtca
     1501 ggataaagcg ccatatagtg tttgatacgc atcagcggcg caagaagcga ttgctcgcca
     1561 cggcgataac tgtcgataag cgccgccagc tgctggcgtt ctgtggaatc aatatgtggg
     1621 cgaaaatatc cctgaacgtg catcaaaacg ttagtatgat cacgccggtt ggcagggtga
     1681 gaaagcaaaa caatcacgcg ttggcggtac tggttgtaat agtcatccag actttgccac
     1741 cggtgaatat cagccacaaa cgggccaagt ttgcggtact ccggctgaga atgcgccaga
     1801 aagacgagtt tatagcggct gtgataatcg agaagcgccc cgcgcgttag tcctcgatct
     1861 ttgagattgt gtaactcatg caaagcaaaa atgcggataa gggtgctgtc atcaaaaagt
     1921 tgttgattca ttactgctcc tgaacggttt acaggagtaa gtgtagacgc cgaaaaaaga
     1981 aaaccgccag cgtttccacc ggcggttttc tttaaacgtc atgtttggca atcagtaaaa
     2041 atcaccggtt gccttttcgg cctgatccat ccacaccggt ttatcgctgg ttttcgacca
     2101 gacgcggtgc aggtagctat aaaagcgggc gcggtctttc cagaaaagca tgaaaggcag
     2161 cgccagaacg cccgctgcaa ccgcacaagt acggcgtaaa aagataagcc atgcaggata
     2221 ttctttgtag agttccatac ttttctcccc cttttgtatc tacccggtga atagcaccgg
     2281 aaaaatgaat tcgtttatct gatgaaaata gtaccgctgt ttgtgtaatt ttactactca
     2341 tccgaccact tatttttgct tattgatggt ttatttatat tcaccgtgta attaagttac
     2401 ataaaagtta aattaatact aaacattagt taaatcattg cttttgccat ttttatactt
     2461 tttttacacc acgcccgcag atttttgcga aatctttgca gccagaactc tacccttccg
     2521 gtatcacttt taggccactg gaggtgcact gtgagtgcag gcgtgataac cggcgtgttg
     2581 ctggtgtttt tattactggg ttatctggtt tatgccctga tcaatgcgga ggcattctga
     2641 tggctgcgca agggttctta ctgattgcca cgtttttact ggtattaatg gtgctggcgc
     2701 gtcccttagg tagcgggctg gcgcggctga ttaatgatat tcctcttcct ggtacgacgg
     2761 gcgttgaacg cgtactcttt agcgcgcttg gcgtctctga ccgcgagatg aactggaagc
     2821 agtatctctc agccattctt ggcctgaata tactggggct ggcggtgctg ttttttatgt
     2881 tgctcggtca gcactatctg ccgcttaatc cacagcagtt gccagggctg tcgtgggatc
     2941 tggcgctcaa taccgccgtc agctttgtca ccaataccaa ctggcaatct tatagcggtg
     3001 aaaccacgtt gagctatttc agccagatgg cgggcttaac ggtacaaaac tttctttctg
     3061 ccgccagcgg gattgcggtg atttttgccc tcattcgtgc atttacccgc cagagcatga
     3121 gcacactcgg gaatgcctgg gtcgatctgc tacgcatcac gttatgggtg ctagtccctg
     3181 tggcgttgtt gatcgcactg ttttttattc aacaaggtgc gctgcaaaac tttctgccct
     3241 atcaggcggt gaataccgtt gaaggagcac aacagctgtt acccatgggg ccagtagctt
     3301 ctcaggaagc gatcaaaatg ctcggaacta acggcggtgg cttttttaat gccaactcgt
     3361 cgcatccgtt tgaaaaccca accgcactga ccaacttcgt gcaaatgctg gcgattttct
     3421 tgatcccaac agcgctgtgc tttgcctttg gtgaagtggc gggcgatcgt cgccaggggc
     3481 gcatgttgct gtgggcgatg tcggtgattt ttgtcatctg cgtaggcgta gtgatgtggg
     3541 cagaagttca gggtaatcct catctgctgg cgctcggcgc ggacagcagc atcaatatgg
     3601 aaggtaaaga gagccgtttc ggcgtgctgg tcagtagcct gtttgcggtc gtgactacgg
     3661 cggcttcctg cggcgcggtg attgcgatgc atgattcgtt taccgctctt ggtggcatgg
     3721 tgccgatgtg gttgatgcaa attggtgaag tggtattcgg cggcgtcggt tccggccttt
     3781 acggcatgat gctgtttgtc ctgctggcgg tgtttattgc cgggctgatg attggtcgta
     3841 caccggaata tctgggtaaa aaaatcgacg tacgcgagat gaaactgact gcactggcga
     3901 ttctggtcac cccgacgctg gtgctgatgg gcgcggcgct ggcaatgatg accgacgccg
     3961 gacgtagcgc catgctcaac cctggcccgc atggttttag cgaagtgctg tacgccgtgt
     4021 catccgccgc taacaacaac ggcagcgcct ttgccggatt aagcgccaac tctccattct
     4081 ggaactgttt actggcgttg tgcatgtttg tcgggcgctt cggggtgatt atcccggtga
     4141 tggcaattgc cggttcgctg gtgagtaaaa agagccaacc cgccagctcc ggcacattgc
     4201 caacgcacgg cccgctgttt gttggcctgt taatcggcac cgtgttgctg gttggcgcac
     4261 tgacctttat ccctgccctg gcgcttggtc cggtggcgga atatctctcc tgatgatatt
     4321 gagtgagcac tgaatatgag tcgtaaacaa ctggcgctat tcgaaccaac acttgtcgtt
     4381 caggcgctga aagaagcggt gaaaaaatta aacccgcagg cgcaatggcg caatccggtg
     4441 atgtttatcg tctggatcgg cagtctgctg accacctgta ttagcatcgc gatggcaagc
     4501 ggtgtgatgc ctggtaatgc gctgtttagc gcggcaatta gcggctggct gtgggtcacc
     4561 gtactgttcg ctaatttcgc cgaggcgttg gcagaaggcc gcagtaaagc gcaggccaac
     4621 agtctgaaag gggtgaaaaa aaccgctttt gctcgcaagc tgcgcgaacc gaaatatggc
     4681 gctgtggcag acaaagttcc tgccgatcaa ctgcgtaaag gcgatatcgt actggtagaa
     4741 gctggcgata ttatcccctg cgatggtgaa gtcattgaag gcggcgcatc ggtcgatgaa
     4801 agcgccatca ccggggaatc ggctccggtg atccgtgaat ccggcggtga ttttgcctcc
     4861 gtcactggcg gcacgcgtat tctttctgac tggctggtga ttgagtgcag cgttaacccc
     4921 ggcgagacat ttctggatcg gatgatcgcg atggtggaag gtgcgcagcg acgtaaaacg
     4981 ccgaacgaga ttgccctgac cattctcctg attgccctga ctatcgtctt tttactggca
     5041 accgccacgc tgtggccgtt ttccgcgtgg ggcggtaatg cagtcagcgt aacggtactg
     5101 gtggcactgc tggtctgtct gatcccaacc actattggcg gcctgttgtc agcgattggc
     5161 gtcgccggga tgagtcggat gctgggcgcg aatgttattg ccaccagcgg gcgtgcagtt
     5221 gaagcggcgg gcgacgtgga cgtcctgctg ctggataaaa ccggcaccat cacactcggt
     5281 aaccgtcagg cgtcggagtt tatccctgcg cagggcgtgg atgaaaaaac gctggctgac
     5341 gccgcacaac tggcttcgct ggctgatgaa acgccggaag gccgcagtat tgtgatcctc
     5401 gccaagcagc gttttaacct gcgcgagcgc gatgtgcagt cgctccatgc cacctttgta
     5461 ccgtttactg cgcaaagccg gatgagcggg atcaacatcg acaaccgcat gatccgtaaa
     5521 ggttctgtcg atgccattcg ccgccatgtt gaggctaacg gcggtcactt ccctgccgat
     5581 gttgatcaaa aagtcgatca agttgcgcgt cagggagcca cgccgctggt ggtggtggaa
     5641 ggttctcgtg tgctgggcgt tattgcgctg aaagatatcg tcaaaggcgg tattaaagag
     5701 cgcttcgccc agctgcgtaa aatgggcatt aaaacggtga tgattaccgg cgataaccgt
     5761 ctgactgccg ccgcgattgc cgcggaagcg ggcgtcgatg attttctcgc cgaagcaaca
     5821 ccggaggcca agctggcgtt gattcgtcag tatcaggcag aaggccgttt agtggcgatg
     5881 accggcgatg gcaccaacga tgctccggcg ctggcgcagg cggatgtcgc ggtggcgatg
     5941 aactccggca cccaggcggc gaaagaagca ggcaatatgg tggatctcga ctccaacccg
     6001 accaagttga tcgaggtggt gcacattggc aaacagatgc tgatgacccg tggctcgttg
     6061 accaccttca gcattgccaa cgatgtggcg aaatacttcg ccattattcc agcggcgttc
     6121 gcggcaacgt atccgcagtt aaatgcgctg aacatcatgc gcctgcattc gcccgactcc
     6181 gccattctca gtgcggtcat tttcaacgcc ttgattatcg tctttttgat tcccctggcg
     6241 ttaaaaggcg tgagttataa accgcttacc gcttctgcca tgttgcgccg taacttatgg
     6301 atttacggtc tgggtgggct gctggtgccg tttattggta tcaaagtcat tgatttactg
     6361 ctaaccgttt gcggtctggt gtgaggttta ccatgagtgg attacgtccg gcattatcga
     6421 catttctctt tctgttattg attactggcg gcgtttaccc gctgctgacc accgcactgg
     6481 ggcaatggtg gtttccctgg caggccaacg gttcattgat tcgtgaaggt gatacggtgc
     6541 gcggttcggc attaatcggg caaaatttta ccggcaacgg ctattttcat ggtcgcccgt
     6601 cggcaacagc agaaatgccc tataatccac aggcttctgg cgggagcaat ctggcagtca
     6661 gtaacccggt gctggataaa cagatagccg cacgcgttgc cgcattacgg gccgctaacc
     6721 cggacgccag cacgagcgtt ccggttgaac tggtgacagc gtcagcaagc gggctggaca
     6781 ataatattac cccgcaagcg gcggcctggc agatcccacg cgtggcgaaa gcgcgtaatc
     6841 tcagcgttga acagctcacg caactgatcg caaaatacag ccaacaaccg ctggtgaact
     6901 atatcggcca gccggtcgtc aacgttgtag aactcaatct ggcgctggat aagcttgatg
     6961 aataacgaac ccttacgtcc cgaccccgat cgtctgctgg aacaaactgc cgcgccgcat
     7021 cgggggaagc tgaaagtttt cttcggtgcc tgcgcaggcg tcggaaagac ctgggcgatg
     7081 ctggcagaag cccagcgact gcgggcgcaa gggctggata ttgtggttgg cgtggtagaa
     7141 acccacgggc ggaaagatac cgccgcaatg ctggaaggtc tggctgttct gccgctgaaa
     7201 cgccaggcgt accgtgggcg gcatatcagc gagtttgatc tcgatgccgc cctcgcccgc
     7261 cgtccggcac tgatcttaat ggacgaactg gcgcacagta atgcgccagg ttctcgtcat
     7321 cctaaacgct ggcaggacat cgaagagctg ctggaagcag gcattgatgt tttcactacc
     7381 gtcaacgttc agcatctgga aagcctgaac gatgtggtca gtggcgtaac cggaattcag
     7441 gttcgggaaa ccgtgcccga tccttttttc gatgccgccg acgacgtagt actggtggac
     7501 ttgcccccgg acgatctgcg ccaacgactg aaagaaggca aagtctatat tgccgggcag
     7561 gcagaacgcg ccattgagca ttttttccgc aaaggcaatc tgatcgccct gcgcgagctg
     7621 gcgctacgcc gtactgccga tcgcgtagat gagcaaatgc gcgcgtggcg ggggcatcct
     7681 ggcgaagaga aagtctggca cacgcgcgac gcgatccttt tatgcatcgg gcataacacc
     7741 ggcagtgaga aactggtccg cgcggcggcg cggctggcgt cgcgcctggg cagtgtctgg
     7801 cacgcggtgt atgttgaaac ccctgccctg catcgcttac cggagaaaaa acgtcgggca
     7861 attctcagcg ccttacgtct ggcgcaggag ttgggcgcgg agacggcaac actttctgat
     7921 ccagcggaag aaaaagcagt agtgcgttat gcccgtgaac ataatctcgg caagattatt
     7981 ctcggtcgcc cggcctcgcg ccgctggtgg cgtcgggaaa cgtttgctga ccggctggcg
     8041 cgcatcgccc ccgatctcga tcaggtactg gtcgcgcttg atgaaccacc cgcccgcacg
     8101 attaataacg cgccggatag ccgctctttt aaagacaagt ggcgggtaca gattcaggga
     8161 tgcgtggttg ccgccgcgtt atgcgccgtt atcaccttaa ttgccatgca gtggctgatg
     8221 gcgtttgatg ccgccaacct ggtgatgctg tatctgcttg gcgtggttgt ggtggcacta
     8281 ttttacggac gctggccttc ggtggttgcg accgtcatta atgtggtgag tttcgatctc
     8341 ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg tgcaatatct gctgaccttc
     8401 gcggtgatgt taaccgtcgg gctggtgatc gggaacctta ctgctggcgt gcgttatcag
     8461 gcgcgggtag cccgttaccg cgaacaacgc acacggcact tatatgaaat gtccaaagct
     8521 ctggctgtgg gccgtagttc gcaggatatc gccgccacca gcgaacagtt tattgcctcc
     8581 acgtttcatg cccgtagcca ggtgttgctg cctgatgaca acggtaaatt gcagccttta
     8641 acacatccgc aaggaatgac gccgtgggac gatgccatcg cgcagtggag ttttgataaa
     8701 ggcctgcctg cgggcgcggg caccgacacg ttacccggtg taccgtacca gattttgccg
     8761 ctaaaaagcg gcgagaaaac ctacggactg gtggtggtgg aaccggggaa tctgcgccag
     8821 ttgatgatcc cggaacagca gcgcctgctg gagacgttta cgctgttagt cgccaatgcc
     8881 cttgagcgac tgacactgac cgccagcgaa gaacaggcgc ggatggcaag cgaacgtgaa
     8941 cagatccgca acgccctgct ggcggcgctt tcgcatgatt tacgcacgcc gcttacggtg
     9001 ctgttcggtc aggcagaaat cttaacgctc gatctggcaa gcgaaggatc gccccacgcc
     9061 cgccaggcca gcgagattcg tcagcatgtg ctgaacacca cccgactggt gaataatcta
     9121 ctggatatgg cgcgaattca gtccggcggc tttaatttga agaaagagtg gttaacgctg
     9181 gaagaagtgg tcggcagcgc gctgcaaatg ctggaaccgg gtttatcgtc gcccatcaat
     9241 ctttctctgc cagagccgct gaccttaatc cacgttgacg ggccactctt tgaacgggtg
     9301 ctgattaacc tgctggagaa cgcggtgaaa tatgcggggg cgcaggccga aattggtatc
     9361 aatgcccaca tcgagggcga aaatctacaa ctggatgtct gggataacgg ccccggtctt
     9421 ccaccaggcc aggagcagac gatatttgat aagtttgctc gcgggaataa agagtcggcg
     9481 gtaccgggtg tagggcttgg gttggcaatt tgtcgggcga tagtggatgt acacggcggc
     9541 accattaccg cattcaaccg accggaaggt ggtgcctgtt ttcgtgttac acttccccag
     9601 caaacagccc ctgaacttga agaatttcat gaggatatgt gacaaacgtt ctgattgttg
     9661 aagatgaaca ggctattcgt cgctttctgc gcacggcgct ggagggcgat gggatgcgcg
     9721 tctttgaggc cgaaacgctg caacgcggct tgctggaagc ggctacccgt aagccagatt
     9781 tgattattct cgatctcggc ctgcccgatg gtgatgggat tgagtttatc cgcgacctgc
     9841 gccagtggag cgcggtgccg gtgattgtgc tttccgcacg tagcgaagag agcgacaaaa
     9901 tcgccgcgct ggatgccgga gcggatgatt atctgagtaa gccgtttggc attggcgaat
     9961 tgcaggcccg tctgcgcgtc gctttacgtc gccactccgc caccgccgcg cccgatccgc
    10021 tggtgaaatt ttccgatgtt accgtcgatt tagccgcccg cgttatccat cggggcgagg
    10081 aagaggtgca tctcaccccg attgagttcc gcctgctggc agtgttgctc aacaacgccg
    10141 gaaaagtgct cacccagcgc cagttactca atcaggtgtg gggaccaaac gcggtcgaac
    10201 acagccacta tttgcgtatt tatatggggc atctgcgaca aaaactggag caagatcccg
    10261 cccgcccccg ccattttatt actgaaaccg gtattgggta tcggtttatg ccatgaatta
    10321 ttattcaata aatacgctta aataattatt aatttattat taaacctgcc ccgaaaatta
    10381 ttatttggca ggtttaattt cttaatgaaa atacaaccca caaaattgca acatttaaac
    10441 aacaaaacag catctaatat ggcgctattc atcattaatg atttattatt tgggatatga
    10501 tcgttttttg ttgatcttct tcacagatta tagccatttc atggatagaa taaccctacc
    10561 ttcaactgac acagaaaaag gtaaaggtaa atggaaaata ataaccgctt aatgcctcat
    10621 ataaggcgga caacccatat catgaagttt gcccatcgta atagttttga ctttcatttc
    10681 tttaatgccc gctagtcttc tgactaaagg gcaccccagc gtacaggtct ccctgacttt
    10741 aagcattaca ggttaatacc tgtatccctc ggtgctcata tactgctaac ccttttaaac
    10801 tctaaataat tcgagtcgca gcacatgcaa cttgaagtat gacgagtata gccagttacc
    10861 gggctggtct gggttattgc atctgcaaaa agcaaactac tgatttattt atcagcggtg
    10921 gggttttgct tttttccggc gtgatcaatt tctcctttga gaaattgagg acctgctatt
    10981 acctgaaata aagagatgaa caaaatgtca gaattaaaaa ttgccgttag ccgttcttgc
    11041 ccggattgtt tttccactca tcgtgaatgc gtgaatattg acaaaagtaa ttatattgac
    11101 gttgccgcca ttattttatc agtcaatgat gttgaacatg gaaaactcga tgaaatagac
    11161 gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg tgtaccagca
    11221 gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa agagttttat
    11281 ggacgtcagt tagaaaccgc tgccagccat tatgaaacgc aactgcgccc gcccttcttc
    11341 cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc gggtcatcag
    11401 ggaggtgagt ttttccgtcg ccatccggca ggcaatcagt tcgtggaata ttttggcgaa
    11461 atgctcttcc gctccgactt gtgtaatgcc gacgttgcaa tgggcgatct actgattcat
    11521 gaaggtgcgc catgcattgc acagcaacat gcggctaaag tgtttaatgc cgataaaacg
    11581 tactttgttt taaacggcac atcctcctcc aacaaagtgg ttttaaacgc cctgctgaca
    11641 ccgggtgatc tggtgctgtt tgaccgtaat aaccataagt ctaaccacca cggagcgttg
    11701 ctacaggctg gtgcaacacc ggtttatctg gaaacagcac gtaacccgta tggttttatc
    11761 ggtggcattg atgcgcactg cttcgaagaa agttatctgc gtgaactgat tacagaagtt
    11821 gcaccgcaac gggcaaaaga ggcgcgtccg ttccgcctcg ctgtgattca gttaggcacc
    11881 tacgacggta ctatttataa cgcccgccag gtggtggata aaatcggcca tctgtgtgac
    11941 tacatcctgt ttgactccgc atgggtcggc tatgaacagt ttattccgat gatggcggac
    12001 tgttcgccgc tgctactgga gcttaatgag aacgatccgg gtattctggt tacgcaatct
    12061 gtgcataaac aacaggctgg tttttctcag acttcacaaa ttcataaaaa agacagccat
    12121 atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt tatgatgcac
    12181 gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc caaaatgcat
    12241 gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg ttaacggtat taatgcccgc
    12301 aaattgatcc tcgataactg ccagcacatt cgcccgttcg ttcctgaact ggtggatggt
    12361 aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt ctttaaattt
    12421 gtacccggtg agcactggca ttcttttgaa ggctacgcag agaaccaata ctttgtcgat
    12481 ccgtgcaaac tgttgctgac aactccaggt attgatgcac gtaacggcga atatgaagcg
    12541 ttcggtgtac ccgcaacgat tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa
    12601 aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat ggccaaactg
    12661 cagcaacttg ttgccctgct ggtacgcttc gaaaaactac tggaagctga tgcgcctctg
    12721 gcggaagtac tgccttccat ctacaaacag catgaagagc gctacgccgg ttataccctg
    12781 cgtcagttgt gccaggaaat gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa
    12841 aaagagatgt tccgtaagga acacttccct cgcgtcagca tgaatccgca agaagccaac
    12901 tacgcctact tacgcggtga agtggagctg gttcgtctgc cggatgcaga aggccgtatc
    12961 gctgccgaag gtgcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc tggtgaaatc
    13021 tggggtggtg ctgttctgcg ttacttcagc gctctggaag aaggcatcaa cctgctgcca
    13081 ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg taagcaagtt
    13141 tggtgctatg tcatcaagcc tcgtgatgcg caaagcgccc tgttgaaagg ggaaaaatta
    13201 tgagtcaggc gaaatcgaac aaaatgggtg tcgttcagtt aaccatactg acgatggtca
    13261 acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtc gggacaatct
    13321 caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg gcattcgcaa
    13381 aatgcggtat gttcagccgt aaatcaggag gtatgggcgg ttatgccgag tatgctttcg
    13441 gtaaatccgg taactttatg gcgaactata cctacggcgt ctcgctgctg attgctaacg
    13501 tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg agtttgtcgc
    13561 cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg gctaactttg
    13621 gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc attattccgg
    13681 tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac gttgattcct
    13741 ggaatccgca tcatgcaccc ttcttcagtg cggtaggttc ttccatcgct atgacgctgt
    13801 gggcttttct tggtctggag tctgcgtgtg cgaatactga tgtagtggaa aacccggaac
    13861 gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct
    13921 ccaccaacgt gattgccggg attgtgccaa atatggagct ggcaaattca acggcaccat
    13981 ttggtctggc cttcgcgcag atgttcacgc cggaagtggg taaagtcatt atggcgctga
    14041 tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc caggtgttta
    14101 aatcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc aaagtggatg
    14161 caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg ctgatgacga
    14221 ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg gtgactaata
    14281 tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc gctaatgtgc
    14341 ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg tacagctttt
    14401 atgcgctgta ctcatccggg gaagaagcca tgctatacgg ttccatcgtg accttcctcg
    14461 gttggacact gtatggtctg gtctcaccac gctttgaact gaaaaataaa cacggttaat
    14521 taagcaaagc aaaaaagggc gatcttccga ccgccctttt tcttatttga tatgtttacg
    14581 cgtttttcag aacttcgcta acaatttcaa ccgcttcttt ctcaatctgc ttgcgatgtt
    14641 cttcaccaag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct gacggacgcg
    14701 cggcgaacca gccgttgtca gtcatcactt tcagaccgcc gatagaagcg ccattacccg
    14761 gtgccgccgt cagacgcgcg gtgatcgggt cacctgccag ggtgctggcg ctcaccattt
    14821 ccggagacag cttagacaac gccgcttttt gtgcggaagt cgctgctgcc tgcaaacggt
    14881 tatagctcgg cgcaccaaag cgttttgcca gttcgttata gtgttcctgc gggttcttac
    14941 cggtgacagc ggtgatttcc gccgccagca gacacataat gatgccgtct ttatcggtgg
    15001 accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc
    15061 cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa acctaccggg acttctacca
    15121 gcttacggcc caagtcattg accacacggt cgatcatcgc agatgaaacc agcgttttac
    15181 cgacagcaac atctttgccc cactgcggac gatgctggaa caggtaattg atcgccaccg
    15241 ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat
    15301 ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg
    15361 cacactcgga ggagcagtcc atacggatcg cgccgtcttt atcaaggtgc ataaagcgga
    15421 aggtttgatc gacctgatcg ttaacaatag tcaggttgag gttgtaatac tcgccaatac
    15481 gcttccagta ttcgataccg gaaccgccca gcggatcaac gccaagcgtc aggcccgctt
    15541 tctgaatcgc cgccatatca acgatatcgg ccagcccttc cacaaacggc tgcaccagat
    15601 cctgctcttt cacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt
    15661 tcaggccatc agccagcagt gcgtttgctc tgtcttccac cactttagtg acgttggtat
    15721 cagccgggcc accatttggc ggattgtact tgataccacc atcttccggc gggttatggg
    15781 acggtgtaat cacgataccg tctgccagcg ggccaccttt tttattgtga accagaatgg
    15841 cattggaaat ggcaggtgtc ggagtgaagc cattgttttc ctgcacaatg acatcaacgc
    15901 cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat
    15961 ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct
    16021 gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac
    16081 cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat
    16141 attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac
    16201 gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc
    16261 ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat
    16321 ttacggccgg tgttggtgtt ggtgatcacc caatacggcg taccaggcac atgtttcggc
    16381 ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta
    16441 cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga atatagtgta
    16501 gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc gtccgaaagc
    16561 agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg
    16621 atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg
    16681 gtaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg
    16741 ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc
    16801 agtgtcttag ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc
    16861 gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac
    16921 aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg
    16981 aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccat
    17041 ggtctgtttg gcagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac
    17101 aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat
    17161 tacccggcga tggcgcagga tcttgttgat accctggatg cacagcagat cgacaaagca
    17221 acatttatcg gtcactccat gggcggtaaa gcggtaatgg ctcttactgc actagcccct
    17281 gatcgcattg acaagctggt ggcgattgat attgcgccgg tcgattatca cgttcgccgt
    17341 catgatgaga ttttcgcggc catcaacgca gtcagtgaat cagacgcaca aacacgccag
    17401 caagcagcgg caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgttg
    17461 aaatcttttg ttgacgggga gtggcgcttt aacgtgccag tattgtggga tcagtatccg
    17521 catattgtgg gttgggagaa aatcccggca tgggatcatc ctaccctgtt tatccctggc
    17581 ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca atttccacag
    17641 gctcgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg
    17701 gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgt
    17761 ccgcgtcgct ttccgcgcgt ggacgttggc gtgactcatc gcgctgatgt attatggcgc
    17821 gctatccatc cgggcagagg cctggctgat tgtttccccc gaagtcacca agatcatggc
    17881 caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg
    17941 accgaaaact aatccgcttt cgcgcgatga acagctgcgt attaataaac gcaaccaact
    18001 aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg cggaagctgt
    18061 cgaggcgctg aacgagctgg cggaatcgcg taatatgagc cgtagcgaac tgatcgaaga
    18121 gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccacttt
    18181 catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct
    18241 atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta
    18301 tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata
    18361 tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa
    18421 aaagcagcaa agaagatctg gaagcatatg acattctgct gctgggcatc ccaacctggt
    18481 attacggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt
    18541 ttaatggtaa actggttgcg ctgtttggtt gtggcgacca ggaagattac gcagaatact
    18601 tctgcgatgc attgggcacc atccgcgaca tcattgaacc gcgcggcgca actatcgttg
    18661 gtcactggcc aaccgcgggc taccacttcg aagcgtcaaa aggtctggct gatgatgacc
    18721 actttgtcgg tctggctatc gacgaagatc gtcagccgga actgaccgct gaacgtgtag
    18781 aaaaatgggt taaacaaatt tctgaagagc tgcatctcga cgaaattctc aatgcctgat
    18841 gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt
    18901 gctacaaatt tgtaactttt gctgttgtac ctgtacaatg tcccggtgtt caagtggcct
    18961 tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgta
    19021 gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag caaattctgt
    19081 cacttcttct aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca
    19141 ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag
    19201 ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg
    19261 atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgacg
    19321 ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc
    19381 aacatcacca cgatcacctg atctgcctcg actgcggtaa ggttatcgaa tttagtgatg
    19441 attccatcga agcgcgtcag cgtgaaatcg ccgcaaaaca tggcattcgc ctgactaacc
    19501 acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat gagcacgcgc
    19561 acgaaggcaa ataagccagc ctgaacgaga aaagccaacc tgcaggttgg cttttttata
    19621 caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta ctgcgaattt
    19681 cctgccagac cttatcacaa tcggctttca ccgcctgatc atttccggtt tgcgttgcct
    19741 gaatgcactg ctgataatcc agtacgcgga cactttcctg ctcggcaaac tgctgatgtt
    19801 gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt tccggcgaac
    19861 cttgcgcggt gttgatacag gcgctgtaag cctctttcag acgactgtct tcctgtggcg
    19921 cggttgattg cgcacaagcc accagccccg atgccatcat ggcgatgaga atcagttttt
    19981 tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcatcgg gcatcagaag
    20041 atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa gatgttgcca
    20101 taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg
    20161 aacatcgtgc ctttggcgcg gccgtcctga atggtgtaga ccgcatccag gctgtaggca
    20221 gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg ccaggtagcg
    20281 ggtttagcat cccatgcgta aacgtaggag gcgccgatgg caaaacctgg aagattccag
    20341 tttttcaggt catacatcgc gccgaagaag accgcttttt cgccgttggc gttgaagtca
    20401 gaacggttat cccaccagat atccaggcga ccgttcgagg aagcgtaggt tggtgtcata
    20461 cgttgcagga agtatccctg ctgaccgtcg gctttaaccc aggtgccttc cagacgcaaa
    20521 tccactacgt cagccgcccg gtaaccaaag gtcaacgcct gcagccaggc ggtgccgtca
    20581 taaagatcgt tggcgctgcg atcgtcaact ttatcgcgcg tgccatagaa ctggtagctg
    20641 gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa atattgatcg
    20701 atataccctt cggcctgacc aaacgccgct tccagtacga agttattttt gaagtcgtac
    20761 ttcgccccaa gggagtgtag ataatcaact ttggtggttt tatcgttctg ataaaactca
    20821 tccatttcca gatgccacgg tgctttgtat tcgttggtcc acatgtagga gaaactcaac
    20881 gcaccagcat cgccgtaatc aaaattcgcc ccggcctccg ccccctgata agtacccggc
    20941 ataaagctcc agtgtggcgc taacagcgtc tgaccggttg gctgaatata acctgccctc
    21001 gcccaaaccg gaccgtattt aaatttggcc gctgctttat acaggcttat accgctttta
    21061 tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat ttcgttcggg
    21121 tgagagctgt cgccgttttc cgccatttca atcgccgtaa acgcggcaat atcaaggccg
    21181 aacatatcag cagcataacc agactgaaaa tcgaggttgg cgttccaggt ggagtgagaa
    21241 aggttggttt tgtatttatc gccgtcggta acatctttac ggtcgcgttc acgctgccag
    21301 taatagatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg agcctccgga
    21361 attgccataa agcccgacat agctgtaaca ccggcgatag ccagcgccag cgtactacgt
    21421 ttgccactaa acgcacgcat gggttaatcc tctttgacgt ataaattgct gcaccaaagg
    21481 tgaacagcga aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca
    21541 ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga
    21601 acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcagttgaa
    21661 aggcttgcag aagaataaaa acgtgaaatt ccagcaagat gaaagcggtt acattttatt
    21721 taa
//
LOCUS       DAOFSV010000068        21491 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.068, whole genome shotgun sequence.
ACCESSION   DAOFSV010000068 DAOFSV010000000
VERSION     DAOFSV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.068"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            16..729
                     /locus_tag="QBH27_002700"
     CDS             16..729
                     /locus_tag="QBH27_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDQ7014995.1"
                     /translation="MQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLT
                     YHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETFLQQHIVQPLGLREDQLIS
                     FHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLNEPGESLQPACHISQLDAR
                     TQQHEMLKTADRPVTRGITLGLKDILNAREVLLLITGEGKQDATERFLTAKVSTAIPA
                     SFLWLHNHFTCVIDEMCRR"
     gene            complement(779..1642)
                     /gene="rsmI"
                     /locus_tag="QBH27_002701"
     CDS             complement(779..1642)
                     /gene="rsmI"
                     /locus_tag="QBH27_002701"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDQ7014996.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            1707..3743
                     /gene="lpoA"
                     /locus_tag="QBH27_002702"
     CDS             1707..3743
                     /gene="lpoA"
                     /locus_tag="QBH27_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDQ7014997.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELVNQLPQELNDNQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVNAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTASPDCVINRKLSWLQYQQGQVVPAS"
     gene            3701..4096
                     /locus_tag="QBH27_002703"
     CDS             3701..4096
                     /locus_tag="QBH27_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDQ7014998.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4116..4706
                     /gene="diaA"
                     /locus_tag="QBH27_002704"
     CDS             4116..4706
                     /gene="diaA"
                     /locus_tag="QBH27_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDQ7014999.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            4716..5291
                     /gene="yraP"
                     /locus_tag="QBH27_002705"
     CDS             4716..5291
                     /gene="yraP"
                     /locus_tag="QBH27_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDQ7015000.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5404..6444)
                     /locus_tag="QBH27_002706"
     CDS             complement(5404..6444)
                     /locus_tag="QBH27_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDQ7015001.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(6517..7152)
                     /locus_tag="QBH27_002707"
     CDS             complement(6517..7152)
                     /locus_tag="QBH27_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDQ7015002.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNVIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7280..7798
                     /gene="yhbO"
                     /locus_tag="QBH27_002708"
     CDS             7280..7798
                     /gene="yhbO"
                     /locus_tag="QBH27_002708"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDQ7015003.1"
                     /translation="MSKKIAALITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVVIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7778..8221)
                     /locus_tag="QBH27_002709"
     CDS             complement(7778..8221)
                     /locus_tag="QBH27_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDQ7015004.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIRFTDNTLGFGKKMIWLRNSGTEQA"
     gene            8272..8574
                     /gene="yhbQ"
                     /locus_tag="QBH27_002710"
     CDS             8272..8574
                     /gene="yhbQ"
                     /locus_tag="QBH27_002710"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDQ7015005.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVTEGAGFAELLSSLQTPEIKSD"
     gene            complement(8561..9064)
                     /locus_tag="QBH27_002711"
     CDS             complement(8561..9064)
                     /locus_tag="QBH27_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ7015006.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLHCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9058..9582)
                     /locus_tag="QBH27_002712"
     CDS             complement(9058..9582)
                     /locus_tag="QBH27_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDQ7015007.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9791..10786
                     /locus_tag="QBH27_002713"
     CDS             9791..10786
                     /locus_tag="QBH27_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDQ7015008.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQHAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10795..11673
                     /locus_tag="QBH27_002714"
     CDS             10795..11673
                     /locus_tag="QBH27_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDQ7015009.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11754..12761
                     /locus_tag="QBH27_002715"
     CDS             11754..12761
                     /locus_tag="QBH27_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDQ7015010.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12879..14123)
                     /gene="mtr"
                     /locus_tag="QBH27_002716"
     CDS             complement(12879..14123)
                     /gene="mtr"
                     /locus_tag="QBH27_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDQ7015011.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIVLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(14277..16166)
                     /gene="deaD"
                     /locus_tag="QBH27_002717"
     CDS             complement(14277..16166)
                     /gene="deaD"
                     /locus_tag="QBH27_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDQ7015012.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16159..16239)
                     /gene="yrbN"
                     /locus_tag="QBH27_002718"
     CDS             complement(16159..16239)
                     /gene="yrbN"
                     /locus_tag="QBH27_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDQ7015013.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16346..17230)
                     /gene="nlpI"
                     /locus_tag="QBH27_002719"
     CDS             complement(16346..17230)
                     /gene="nlpI"
                     /locus_tag="QBH27_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDQ7015014.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17339..19474)
                     /gene="pnp"
                     /locus_tag="QBH27_002720"
     CDS             complement(17339..19474)
                     /gene="pnp"
                     /locus_tag="QBH27_002720"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDQ7015015.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKNAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19721..19990)
                     /gene="rpsO"
                     /locus_tag="QBH27_002721"
     CDS             complement(19721..19990)
                     /gene="rpsO"
                     /locus_tag="QBH27_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDQ7015016.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20139..21083)
                     /gene="truB"
                     /locus_tag="QBH27_002722"
     CDS             complement(20139..21083)
                     /gene="truB"
                     /locus_tag="QBH27_002722"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDQ7015017.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21083..21484)
                     /gene="rbfA"
                     /locus_tag="QBH27_002723"
     CDS             complement(21083..21484)
                     /gene="rbfA"
                     /locus_tag="QBH27_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDQ7015018.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
BASE COUNT         5227 a         5933 c         5496 g         4835 t
ORIGIN      
        1 aaggaatttc atcctgtgca aacccttcaa caagttgaaa actatacggc gttaagtgaa
       61 cgtgccagcg aatatttatt ggccgtgatc cgtagcaaac cggatgccgt gatttgcctg
      121 gcgaccggag ccacgccatt actgacgtat cattatttgg tagaaaaaat ccaccagcag
      181 caggttgatg tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
      241 acgatgcctg gtacctgcga aactttcctt caacagcata tcgtgcagcc gctggggcta
      301 cgtgaagacc agctcatcag ctttcactcc gaagagataa atgagacaga gtgcgaacgg
      361 gtaacgaacc tgattgcgcg caaaggcggt ctggacttat gcgttctcgg attggggaaa
      421 aacggtcatc ttgggctgaa cgaaccggga gaaagcctgc aaccggcctg ccatatcagt
      481 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccgctgaccg tcccgtgact
      541 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaagtttt gttactgata
      601 actggcgaag gaaaacagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
      661 atcccggctt catttttatg gctgcataac catttcacat gcgtgattga tgaaatgtgt
      721 cgtcgttaac tcgcctggta caggcgttgc agcgctattc ctgctgcgac gcctgtaatc
      781 actctccctg ctgctccagc gcatacttat acagcgcatt tttcttcacg ccgtgaattt
      841 ctgcggccag cgccgccgct tttttcagcg gcagttctgc ctgtaacagc gccagcgtgc
      901 gcagggcatc agcaggcagg tcatcttcct gcgctttatg gccttcgaca atcagcacca
      961 tttcgccttt gcgacggttt tcatcttctt ttacccacgc cagcagctcg ccaacgggcg
     1021 cgccgtgaat ggtttcccag gttttggtta gctcacgcgc cagaaccacg taacgggatt
     1081 cgcctaatac cgcaacaata tcttccaggc tatctaacag acggtgggta gattcataaa
     1141 aaatcagcgt gcgcggctcc gcttcaatag cttttagcgc atcacggcgg ccttttgatt
     1201 tggcaggtaa aaagccttca taacaaaaac gatcggatgg taaacccgcg gcgcttaacg
     1261 cagtgatagc agcacacggc cctggtagcg gcaccacgcg aatccccgct tcacgacagg
     1321 tacgcaccag atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg
     1381 caatgttttg cccctcttgc agcttcgcca gcagcgtttc ggctttttgt tgttcgttat
     1441 gatcgtgcag cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt
     1501 gacgcgtatc ctcggcggca atcagatcaa cggcctgtaa tacctctaat gcacgctggg
     1561 tgatatccgc cagattgccg attggcgtcg gtacaatgta aagctgcccc tgagaattat
     1621 ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc
     1681 gtaaaaaaaa tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg
     1741 cgcgttgtct gcctgttgtt ttggcagccc tgattttcgc cggttgtggc acccatactc
     1801 ccgatcagtc cactgcttat atgcagggca cggcgcaggc tgattctgct ttttacctgc
     1861 aacagatgca gcaaagttct gatgatacca ggatcaactg gcaattactc gccattcgtg
     1921 cactggtgaa agaaggtaaa accgggcagg cggttgagtt ggttaaccaa ctaccgcaag
     1981 aactgaatga taatcagcgt cgcgagaaaa cactgctggc ggcagagatt aaactggcgc
     2041 agaaagattt tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc gatttagaac
     2101 aaaaccagca agcccgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca
     2161 ttgatttact gcgcgcgtta attgctcagg aaccgctgct cggtgcgaaa gaaaagcagc
     2221 agaatattga cgccacatgg caggcgctct cctccatgac tcaggagcag gcgaatacgc
     2281 tggtgatcaa cgccgacgaa aatattctgc aaggctggct ggatttgcag cgcgtctggt
     2341 ttgataaccg taacgatcct gacatgatga aagctgggat cgccgactgg cagaaacgtt
     2401 atccgaacaa cccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta
     2461 aaccagcatc gaccaacaaa atcgccctgc tgttgccgct gaatggccag gcagcggtat
     2521 ttggtcgcac tattcagcaa ggctttgaag cggcgaaaaa tatcggcact cagccagtgg
     2581 cggcccaggt agctgccgca cctgccgcag acgtagcaga acaacctcag ccgcaaaccg
     2641 tggatggcgt tgccagcccg gcacaagctt cggttagcga tctgaccggt gaacagcctg
     2701 cggcccagcc ggtacctgta agcgccccgg cgacaagcac cgcagcggta aacgcacccg
     2761 caaatccatc cgcagagctg aaaatctacg atacctcatc acaaccactt agccagatct
     2821 taagccaggt tcagcaggat ggtgcgagta ttgtggtcgg tccgttgctg aaaaataacg
     2881 ttgaagagtt gctgaagagc aacaccccgc tgaacgtgct ggcactgaac cagccggaga
     2941 atatcgaaaa ccgcgtcaat atttgttact tcgcgctttc gccagaagac gaagcgcgcg
     3001 atgcagcgcg tcatattcgt gaccagggta aacaagcgcc gctggtgctg atcccgcgca
     3061 gttcattggg cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa ctgggtggcg
     3121 gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt gttaacggcg
     3181 gttctggtat tgctttaacg ggtagcccga ttactcccag agcgacaacc gactccggca
     3241 tgacgaccaa caatccaacg ctgcaaacca cgccaaccga tgaccagttc accaataatg
     3301 gcggtcgtgt cgatgcggtg tacattgtgg caacgccggg tgaaatcgct tttatcaaac
     3361 cgatgatcgc catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc agttcccgca
     3421 gtgcgcaagg gaccgctggc ccggatttcc gcctggagat ggaaggcttg cagtacagcg
     3481 aaatcccgat gctggcaggc ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga
     3541 acaacgatta ttcgctggct cgcatgtatg cgatgggtgt cgatgcctgg tcgttggcaa
     3601 atcatttctc acaaatgcgc caggttcagg gttttgaaat caacggtaat accggaagcc
     3661 tgacggcaag cccggattgc gtgattaaca ggaagttatc atggctacag taccaacaag
     3721 gtcaggtagt ccccgccagt taaccaccaa acagaccggc gatgcgtggg aagcacaagc
     3781 gcgtcgctgg ctggaaggca aaggactacg gtttatcgcc gctaacgtga acgagcgtgg
     3841 cggcgagatc gatctgataa tgcgtgaagg ccggaccacc gtttttatcg aggtgcgtta
     3901 ccgccgctct gcgctttatg gcggcgcggc agccagtgtg acccgcagca aacaacacaa
     3961 attattacag actgcccgtt tgtggctcgc gcgtcataat gggagttttg atactgtgga
     4021 ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta aggatgcctt
     4081 taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt aaagcttgct
     4141 tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat gccatctccc
     4201 gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc tgttgtggta
     4261 atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac cgtttcgaaa
     4321 cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc ttaacggcaa
     4381 ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg
     4441 cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt gtaaaagcgg
     4501 tagaagctgc cgttacgcgt gatatgacca ttgtggcatt aaccggctat gacggcggtg
     4561 aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg catcgtagtg
     4621 ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg atcgataaca
     4681 cgcttttccc tcaccaggat gtttaaggag aatacatgaa ggcattatcg ccaatcgcag
     4741 tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg ggtactgctg
     4801 ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggta gacgatggta
     4861 ccttggaagt gcgcgtgaac agcgcattgt cgaaagacga acaaattaag aaagaagcgc
     4921 gcattaatgt aacggcctat cagggcaaag tgctgctggt tgggcagtca ccaaacgctg
     4981 aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac gaagtgtata
     5041 acgagattcg tcagggccag ccgattggtc tgggcgaagc gtctaacgat acgtggatca
     5101 ccaccaaagt acgttcgcag ctgttaacca gcgacctggt gaaatcgtcc aacgtgaaag
     5161 tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt gaagcgaaag
     5221 cggcggcaga tattgccagc cgggtaagcg gcgtgaagcg ggtaactacg gcgtttacgt
     5281 ttattaaata gcagcccttg taatgcctga tgcgacgctt gcagcgtctt atcaggccta
     5341 caacgatgca aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc
     5401 aaatcaaact aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc
     5461 cgtcagccat aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact
     5521 caccgccggg agcgtcatca acaatgccag cgccggagca gttcccatac ctgccagcat
     5581 catcgtttgt acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgccac
     5641 cgccatcgcc accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa
     5701 ccagacgcga gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca
     5761 gaaaagcgtc catagcgccc tgccccagcg gctaaaaaat cctccctgtg cttccggtat
     5821 atcaatttcg accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt
     5881 cgcaatcagc aacaccatca ccagccccgc caccagacga atcgccgcaa aaccccagcc
     5941 gaggacgaac cccataaaca ccagcgtcgc cgggtttaac aacggattcc ccatccagaa
     6001 tgccagcgca ccgcccatcg acacctgttg gcgacgcatt cccgccgcga ccggagccgc
     6061 acagcaggta cacatcatac ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa
     6121 gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg atcagtacct gaatcaacga
     6181 accgaggatc acccccagca ccgccgcttt ccataccgcg aggaaataga tcatcgcgta
     6241 atccaacgcc gcctgccacg gattagcatc cgcctgcgca aggatagatt taccgatact
     6301 gtgggtttcg gcagcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag
     6361 accggcaatg acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc
     6421 ctgagatgaa gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc
     6481 gtgaatatta cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc
     6541 tgaagagctt aaaatcgtca caccttcatg ctcaggccgc atcgcctctg ccagcatgac
     6601 ccgcgccacg tctctcgcat caatggattt ccagttaccg ggtaacaaac ggaacagcgg
     6661 cgcaaaaagc gtttcgttca tccgctgttt gctacgatca cccagtaaca tcgacgggcg
     6721 agcaatggtc aatttcggcc agttctgggc gattaatgct tcttccatct cccctttgac
     6781 gcggttatag aaaaacggcg agtgggcatt agcacccata gcactgacca ccagcatatg
     6841 ctgcgcaccg agccgccgcc cggttaatgc ggtatccacc accaacgtgt aatcggcatg
     6901 aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat
     6961 gtcgatggga tcggtgacct gcgccagcgc gtcagtcagt tgcggatcat ggggattaaa
     7021 caccccgggc atatcgccca acggacgtcg cgtcggcgca gcaatgacgt taactttcgg
     7081 ttcattaatc aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag
     7141 tacctgactc atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc agctcatgca
     7201 ccaggcttaa tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat
     7261 gcaacaacgc ggaggaagca tgagtaagaa aatcgccgct ttaatcactg atgaatttga
     7321 ggattcagaa ttcacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac
     7381 cattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtggtcat
     7441 cgataaatcc atcgatgaag tgacgcccgc agagttcgat gccctgctgc taccgggcgg
     7501 tcattcacca gattatctgc gtggcgacaa ccgttttgtc accttcactc gcgattttgt
     7561 gaacagcggc aaacctgttt tcgccatctg tcacggtccc cagttgctga tcagcgccga
     7621 tgtgatccgt ggtcgtaagc tgaccgccgt taaaccgatc gtgattgatg ttaaaaacgc
     7681 gggcgcggaa ttttacgatc aggaagtggt ggtcgataaa gatcagctgg ttaccagccg
     7741 gacaccggac gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt
     7801 tacgtagcca aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ctgatttcat
     7861 cgagccggat ttcccatacc ggggccgaca gcattctggc aaccggaaag cgacgattgt
     7921 acgccttgcg tgcgaggtcg ctttcctcgc cttccagtct gcggatctca cctttaaact
     7981 gcacgccgcg aattaacgct accgttttcg gctgaccgtt taccgttccg gcaattgccg
     8041 cctgcggccc gctcatctgc gcatgacgcg ttttttcttc cgtcaaaatg tagaaggcga
     8101 ctttctgcgc atcaaaaaga taaaaggcat tggcgcacca cagttcccct tcctgctgca
     8161 cacaccaggt gacaacatgt tgttttgcca gccaacggct gatggcggtg agtgtttcca
     8221 ttgctgttct cttttatact gtgggcctga ccttaacatg cgacgcagac gatgacaccc
     8281 tggtttcttt acttgatccg taccgccgac aataagcttt ataccgggat caccacggat
     8341 gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgagggaaa
     8401 ggagaactaa cgctggcgtt ttccgcgcca gtgggcgacc gttcgctggc actacgagcg
     8461 gaatatcgcg ttaaacaact gactaaacgg caaaaagagc gtctggtaac ggaaggcgca
     8521 gggtttgcag aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct
     8581 cgtgatactc gaccagacca gttacgccgt tcagcgcgtc atccgctaaa cgatgtacct
     8641 ggaaggcgct ttcagtgccc ggccagcggc aatgcagatc gtgatgcgcc gccagttcaa
     8701 agccgaaacg gctgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact
     8761 cattaagcga atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt
     8821 tttcatcaac cgccagcggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa
     8881 ccggactaaa agccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca
     8941 gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga
     9001 atgaacgacg cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta
     9061 gcatggttca cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac
     9121 aaaatccgcc agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat
     9181 ggcgtccatc aggtttttca catacagccc cagctccgta tcgccttcaa tcaccagccg
     9241 acgctggaag aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct
     9301 ggcgtcggca ctaaaactca catcagcttg cgcgttctgg ctaacgacca gtttgccatt
     9361 caccaccgag gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcgacc
     9421 ttcaagaaac tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg
     9481 ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga
     9541 tggccccaaa tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt
     9601 caacaatcat cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc
     9661 catatgaaat tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc
     9721 agcaaggtta actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc
     9781 aggaaaaatt atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat
     9841 cgaaaacggc gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt
     9901 cgccggcctt aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca
     9961 tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat ccggacggtt acgcccgttg
    10021 gcagcacgcc gtggatatgg cggcgcaact gggtgccgac gcgctgatcc tcgccgacct
    10081 cgccatgctg gagtacgccg ccgaacgtta tccgcatatt gagcgccacg tatcggtgca
    10141 ggcttcggcg accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg
    10201 cgtggtactg ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggtcacacc
    10261 tgtaccactg gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta
    10321 tctgtcgtcg tatctgacgg gtgaatcgcc caacaccgtg ggggcatgtt ctccggcccg
    10381 tttcgtgcgc tggcagcaaa cgccgcaagg gctggaatcc cgcctgaacg aagtgctgat
    10441 cgaccgttat caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct
    10501 ggtggacggc gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact
    10561 gctgccggag ttaatggcgg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag
    10621 cccggcgtat gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc
    10681 cgatccgcaa aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga
    10741 aggcacgcaa actacccttg gcgcgtatca ccgtaaatgg cagtgagaaa agcaatgaaa
    10801 tattccttag ggccggtgct atggtactgg ccaaaagaga cgctggaaga cttttatcag
    10861 caggccgccg ccagcagcgc agacgtgatt tatcttggtg aagcggtatg cagcaagcgc
    10921 cgggcaacca aagttggcga ctggctggag atggcaaaat cactcgccgg aagtggtaag
    10981 cagattgtgc tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa
    11041 cgctatgttg agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg
    11101 tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg
    11161 acgctgaaaa tattgcttaa acagggcatg atgcgctggt gtatgccggt ggagctttcc
    11221 cgcgactggc tggtgaatct gcttaatcag tgcgatgagt tgggcattcg caaccagttt
    11281 gaagtggaag tcctgagcta cggtcatcta ccgctggcct actccgcacg ctgctttacc
    11341 gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac
    11401 gggcgcaacg tactgtcgca ggaaaaccaa caagtgtttg tactcaacgg cattcagacc
    11461 atgagcggct acgtttacaa cctcggtaac gagctggcat ccatgcaggg gctggtcgat
    11521 gtggtccgcc tgtcaccgca gggtactgac actttcgcaa tgctcgatgc cttccgcgct
    11581 aatgaaaatg gcgctgcgcc actaccgttg acggcaaaca gcgactgtaa cggctactgg
    11641 cggcggctgg ccgggctgga gctgcaagcg taagtatata gctcactttg ttaacaactt
    11701 taactactct ttaatgcagt attaaagact aatcggtaac aaagtgagct gttatgactg
    11761 ataaaaccat tgcgttttcg ctactcgatc tggcccccat ccccgaaggt tcttcagcgc
    11821 gggaagcgtt ctcccactct ctcgacctcg cccgtctggc tgaaaagcgc ggctatcatc
    11881 gctactggct ggcagaacac cacaatatga ccggcattgc cagtgctgca acgtcggtgt
    11941 taattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt
    12001 tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgcttaat acgctctatc
    12061 cggggcgaat cgatttgggg ctgggtcgcg cgccgggtag cgaccaacgg acaatgatgg
    12121 cgctacgtcg tcatatgagc ggcgatattg ataatttccc tcgcgatgtc gcggaactgg
    12181 tggactggtt tgatgcccgc gatcccaatc cgcatgtgcg ccctgtaccg ggctatggcg
    12241 agaaaatccc ggtgtggttg ctaggctcca gcctttacag tgcacaactg gcggcgcagc
    12301 ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgctgttc caggcgctgc
    12361 atctttatcg cagcaacttc aaaccgtcgg cacggctgga gaaaccgtat gcgatggtgt
    12421 gcatcaatat tattgccgcc gacagtaacc gcgacgctga gtttctgttt acctcaatgc
    12481 agcaagcctt tgtgaagctg cgtcgcggcg aaaccgggca actgccgcca ccgattcaaa
    12541 atatggatca gttctggtca ccgtccgaac agtatggcgt gcagcaggcg ctaagtatgt
    12601 cgctggtggg cgataaagcg aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg
    12661 acgccgatga gattatggtt aacgggcaga ttttcgacca ccaggcacgc ctgcattcgt
    12721 ttgagctggc gatggatgtg aaggaagagt tgttggggta gtgtgtttta acgtaggaag
    12781 ccttatccga gatggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat
    12841 tgtgtaggca gcagaaatgt cggataaggc acctctgatt actgatacac cggcagcaca
    12901 ttaaagctcg ataagatatg caccagtgcg ttgccgacgc caaacaccag aatcagcaca
    12961 atcatcggct tgccgcccca tacacggaat ttcgggctac caaagcgttt acgcgatgcg
    13021 cgggccagca gcgccggaac aattgccgcc cagatggtag ccgctaagcc agcataacca
    13081 atggcgtaca ggaatccgtt aggaaacagc aggccaccca caaccggcgg ggcaaaggtc
    13141 agcaacgctg ttttcaaacg gcccacagcc gagtcgtcga aaccaaacag atctgccaga
    13201 tagtcaaaca ggcccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac
    13261 acaaccagca gaaggtccag gctacggctg ttcagtacgc cgcttaacgc ctgtaccagc
    13321 acatcaatat taccgccctt ctcggcgatg ccgataaact ccggacgcgg gatgttaccc
    13381 atcgtcgcca gcaaccagat ggtatacagc gccagcgcca tcagcgtacc gtacaccagg
    13441 catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg tacgttaccg
    13501 tgataaccaa acgatgccag acagaacggc agcgtcatca acagatacgg tgcataagac
    13561 gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg
    13621 aaggtgagga agaaggtaat gactttcgcc cccagcacaa tcgctgtcat gcgactgacg
    13681 gctttggtgc tcaaccacac cacaaacgcc accaacagtg caaaaccaaa acccgctgcc
    13741 cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca
    13801 gaaatatagg cataggtcag gatgtagagc acaaaggcaa tggaaatgcc gttgaccacg
    13861 ttccagcctt tgccgagcag gtctttggtg atggtgtcaa agctcgaacc gattcggtaa
    13921 ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca ggtgaaaatc
    13981 agcgccgcca ttgaccagaa gaaccacgcc ccggacatga ccactggcag agaaaacatc
    14041 cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt
    14101 tgggtggtgg ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt
    14161 tgttatgcat tgcactgtac cagtacatga gtacaaaaaa cagaaaaaaa gccccgatgg
    14221 gtaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac
    14281 gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca
    14341 cgacgttcgc cgctaaaacg acgcccatca ccacggccac cttcacggcg ttcaccgctg
    14401 aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc acgacgctca
    14461 ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcattggctt gttgagaatg
    14521 cgagtacgcg taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg
    14581 gagtgagaag caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta
    14641 gcaatcgcgc caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatacgg
    14701 tacaactgca tatcgccaac atcacgacgt tcgcgacgcg gacgatcttc acggtcgcca
    14761 cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt
    14821 ttcggacgca tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc
    14881 agtagtgcgg ctgccagagt ttcgagatcc agctcttcac cttcggcagt cggctgaatt
    14941 ttgctcagca gtgcacggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta
    15001 gcggcaaatt tttccagacg acgtttgccc agcagttctg cgttcggcag ttctacttcc
    15061 ggaatggtca gcttcatggt gcgttcgatg ttgcgcagca gacgacgctc acggttctca
    15121 acgaacagca gtgcacgacc agcacgacct gcacgaccgg tacgaccgat acggtgaacg
    15181 taagactcag aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc
    15241 aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc tttcaggcgt
    15301 tccagtgtct gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag
    15361 ccgttacgct caagagcttc tgccacttcc agagtcgcgt ttttggtacg aacgaagata
    15421 atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata
    15481 ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaatg
    15541 cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg
    15601 gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg
    15661 tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt
    15721 ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg tgtaccgaca
    15781 acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgttg gccgccgtac
    15841 agggcaacca cgtttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc
    15901 tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga
    15961 tcaagattct gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc
    16021 atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat
    16081 ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct
    16141 gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag
    16201 tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca
    16261 aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa
    16321 gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt
    16381 cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt
    16441 tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt
    16501 agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatccg
    16561 ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact
    16621 cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt
    16681 ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag
    16741 gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac
    16801 cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct
    16861 caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata
    16921 tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat
    16981 cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa
    17041 gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca
    17101 gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc
    17161 aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa
    17221 aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct
    17281 aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt
    17341 actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg
    17401 cttctttaat gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta
    17461 cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttatca gcgatttgag
    17521 agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga
    17581 ttcgggtcac tttaccattg tagacgcggc ctacttcgat ttctgcagtg atctcttcga
    17641 tacgacgaat agcgtttttc gccttctcgc cgtcggtcgc tgcgatcttc acagtaccgt
    17701 catcttcgat ttcgatggta gtgccagttt cttcggtcag agcacggatt acagaaccgc
    17761 ctttaccgat aacgtctttg atcttgtctg gattgatctt gatggtatgg atacgcggag
    17821 cgaactcaga gatatcaccg cgcggcgcat tgatcgcctg ttccattacg cccaggatat
    17881 gcagacgcgc acctttagcc tggttcagtg caacctgcat gatctctttg gtgatacctt
    17941 caattttgat atccatctgc aatgcagaga taccgtcacg agaacccgca actttgaagt
    18001 ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc
    18061 cttctttcac cagacccatt gcgatacccg caacggcagc tttgatcggc acaccggcgt
    18121 ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcgg
    18181 tgatttcaga cacaacacgt acggtgtacg ggaatttgtc catatccggc attactgcca
    18241 gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca
    18301 tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac
    18361 gttcgcccat cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa
    18421 ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacac
    18481 cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc
    18541 cggccagtac gcggctacga acaacatttt tctcgatagc gtgcagaatt tcacccagtt
    18601 cgttttcgtc cagggtttcg tcttcagcaa gcagtgtcgc gatggtttca gatttgatga
    18661 catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag
    18721 cttcagccag cgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccaat
    18781 cccaacgcgg tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct
    18841 gttgttcatg accgaacacc actgcgccca gcatttggtc ttcgctcagc agttcagctt
    18901 cagattcaac catcagcacg gcagcttctg taccggcaac aaccaggtcc agtttgctct
    18961 ctttcagttc gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacacggg
    19021 cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa
    19081 tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg gcgataacct
    19141 gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg
    19201 cgatcagggt ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac
    19261 gaccagcagc gtaggtacgc tcctgatagt taacggtcag cgggaagaag tcctgacctg
    19321 gtttggcttt tttctgacca acaacggtaa caaataccgc ggtgtcatcc atgctaacca
    19381 taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc
    19441 cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac
    19501 agtaccttac ggtactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca
    19561 accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg
    19621 aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa
    19681 aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc
    19741 gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac
    19801 gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg
    19861 caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcggtag
    19921 aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac
    19981 ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag
    20041 cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat
    20101 tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc
    20161 aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaacttgccg
    20221 ttctcaccct ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg
    20281 ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccactgg gtagtccgaa
    20341 gctggactgt ccattggcat cagtaatggg tcaagtaact ccgcagccgg aatatcctgc
    20401 tgttcggctt gctcaaccag ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga
    20461 tatttactta ccgccagacg acgcaagtaa ataacatgcg cgccacaacc gagtttttcg
    20521 ccgagatcgt caatgatagt gcggatataa gtgccttttg agcagtgaat ttccagctcc
    20581 agctcattgc cttcatggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca
    20641 cgtggaactt caatgccctg acgcgcgtat tcgtacagtt ttttgccctg atatttcagt
    20701 gctgaataca tcgaaggaat ctgttcgata tcgccacgga aggtatccag cgccgccgcc
    20761 agttgctctg cactaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa
    20821 gtatcggtac gctgtccaag acgcgcaatg acccgataac gtttgtcgga gtccagcaga
    20881 tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgccggt cgccagcggg
    20941 tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc
    21001 gcatcgttgc tggacatccc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga
    21061 ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt
    21121 cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag
    21181 agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca
    21241 ggctgcggat gaaaccagaa gcttcctgca acgctttgat gcccgcttta accgcgtctt
    21301 catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt
    21361 cgacaccgga aacggtggtc atcatgccca gacgaggatc tttaatttca cgctgcagga
    21421 tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg
    21481 ccataataaa t
//
LOCUS       DAOFSV010000069        21484 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.069, whole genome shotgun sequence.
ACCESSION   DAOFSV010000069 DAOFSV010000000
VERSION     DAOFSV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.069"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1364)
                     /gene="mnmE"
                     /locus_tag="QBH27_002724"
     CDS             complement(<1..1364)
                     /gene="mnmE"
                     /locus_tag="QBH27_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDQ7015019.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1470..3116)
                     /gene="yidC"
                     /locus_tag="QBH27_002725"
     CDS             complement(1470..3116)
                     /gene="yidC"
                     /locus_tag="QBH27_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDQ7015020.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3119..3376)
                     /gene="yidD"
                     /locus_tag="QBH27_002726"
     CDS             complement(3119..3376)
                     /gene="yidD"
                     /locus_tag="QBH27_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDQ7015021.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3340..3699)
                     /gene="rnpA"
                     /locus_tag="QBH27_002727"
     CDS             complement(3340..3699)
                     /gene="rnpA"
                     /locus_tag="QBH27_002727"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDQ7015022.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3716..3856)
                     /gene="rpmH"
                     /locus_tag="QBH27_002728"
     CDS             complement(3716..3856)
                     /gene="rpmH"
                     /locus_tag="QBH27_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDQ7015023.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4086..4166)
                     /locus_tag="QBH27_002729"
     CDS             complement(4086..4166)
                     /locus_tag="QBH27_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015024.1"
                     /translation="MTIDKKWLNRSNKDPGRSLRFTRQPV"
     gene            4463..5866
                     /gene="dnaA"
                     /locus_tag="QBH27_002730"
     CDS             4463..5866
                     /gene="dnaA"
                     /locus_tag="QBH27_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDQ7015025.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5871..6971
                     /gene="dnaN"
                     /locus_tag="QBH27_002731"
     CDS             5871..6971
                     /gene="dnaN"
                     /locus_tag="QBH27_002731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDQ7015026.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            6971..8044
                     /gene="recF"
                     /locus_tag="QBH27_002732"
     CDS             6971..8044
                     /gene="recF"
                     /locus_tag="QBH27_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDQ7015027.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8073..10487
                     /gene="gyrB"
                     /locus_tag="QBH27_002733"
     CDS             8073..10487
                     /gene="gyrB"
                     /locus_tag="QBH27_002733"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDQ7015028.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10631..11029
                     /locus_tag="QBH27_002734"
     CDS             10631..11029
                     /locus_tag="QBH27_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDQ7015029.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11143..11955
                     /gene="yidA"
                     /locus_tag="QBH27_002735"
     CDS             11143..11955
                     /gene="yidA"
                     /locus_tag="QBH27_002735"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDQ7015030.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(11999..12655)
                     /locus_tag="QBH27_002736"
     CDS             complement(11999..12655)
                     /locus_tag="QBH27_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015031.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            12934..13623
                     /gene="dgoR"
                     /locus_tag="QBH27_002737"
     CDS             12934..13623
                     /gene="dgoR"
                     /locus_tag="QBH27_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDQ7015032.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13620..14498
                     /gene="dgoK"
                     /locus_tag="QBH27_002738"
     CDS             13620..14498
                     /gene="dgoK"
                     /locus_tag="QBH27_002738"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDQ7015033.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSIPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAVDAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRNIAHAVAN"
     gene            14482..15099
                     /gene="dgoA"
                     /locus_tag="QBH27_002739"
     CDS             14482..15099
                     /gene="dgoA"
                     /locus_tag="QBH27_002739"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDQ7015034.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPNIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15096..16244
                     /gene="dgoD"
                     /locus_tag="QBH27_002740"
     CDS             15096..16244
                     /gene="dgoD"
                     /locus_tag="QBH27_002740"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDQ7015035.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16319..17656
                     /locus_tag="QBH27_002741"
     CDS             16319..17656
                     /locus_tag="QBH27_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015036.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17617..18717)
                     /gene="cbrA"
                     /locus_tag="QBH27_002742"
     CDS             complement(17617..18717)
                     /gene="cbrA"
                     /locus_tag="QBH27_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDQ7015037.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            18818..20032
                     /locus_tag="QBH27_002743"
     CDS             18818..20032
                     /locus_tag="QBH27_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDQ7015038.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRASPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            complement(20036..20368)
                     /locus_tag="QBH27_002744"
     CDS             complement(20036..20368)
                     /locus_tag="QBH27_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDQ7015039.1"
                     /translation="MIKNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     misc_feature    20608..20680
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20674..21087
                     /gene="ibpA"
                     /locus_tag="QBH27_002745"
     CDS             20674..21087
                     /gene="ibpA"
                     /locus_tag="QBH27_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDQ7015040.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21199..>21484
                     /locus_tag="QBH27_002746"
     CDS             21199..>21484
                     /locus_tag="QBH27_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HDQ7015041.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSL"
BASE COUNT         5072 a         5522 c         5839 g         5051 t
ORIGIN      
        1 tacttaccga tacagaagct ggagaaaatc cgccccagta ggtcgtctga agtaaactcc
       61 ccggtgatct cgcttaagtt ctgctgcgcc aggcgcaact cttccgccag cagttcaccc
      121 gcccaggcac ctaacagttg cgctttacct tgctgcaaat gctctgccgc ctgttccagc
      181 gcctgtaggt gacgacgacg cgccaggaag ccgccttcca tattagtgtc aaagcccatg
      241 ctctgtttga gatggttacg cagcacatcc acgccttcgc cagtccgcgc cgaaagacga
      301 attaacgcgt gaccgttcac ttcgctcatc cccaacgttt cgccggtgat atcggcttta
      361 ttgcgcacca cggtgatcgg cagtttcgct ggcagacggg cgataaattc cggccagatc
      421 tctgccggat caacggcgtc tgtcgtggtg ccatcgacca taaacagcac gcggtctgcc
      481 tgttcaattt cctgccatgc gcgctcgata ccaatacgtt caacttcgtc actggcttca
      541 cgtagcccgg cggtatcgat gatatgcaac ggcattccgt caatgtggat atgctcacgc
      601 agcacgtcac gcgtggttcc ggcgatatcg gttacgattg ctgcttcacg gcccgccagc
      661 gcgttcagca ggctcgattt accggcgtta ggacgtccgg caatcaccac tttcatccct
      721 tcgcgcaaca aactaccctg acgagcttcg gcgcgtaccg cgtcgagatc ggcaattacg
      781 tcattgagct gggcttcaat tttgccgtcg gagaggaaat cgatctcttc atccgggaaa
      841 tcaattgccg cttcgacgta aatgcgcaag tgggtgaggg cttccaccag atggttgacc
      901 cgtgcggaga acgcgccttg cagcgagttt agcgccgaac gggccgcctg ttccgagctg
      961 gcgtcgataa gatccgcaat cgcctcggcc tgggctaagt cgagtttatc gttcagaaat
     1021 gcgcgttcgg aaaactcacc agggcgggca atccgcaggc cgggaatggt cagaatgcgt
     1081 tttaacagca ggtcgaggat caccggaccg ccatgacctt gcagttccag cacatcttcg
     1141 ccggtgaacg agttcgggcc agggaaccat agcgcaatcc cctgatcgag cacgctgcca
     1201 tcggcgtctt taaacggaag ataatcggcg tagcgtggct taggcagttt acccagcacg
     1261 gtttcggcaa cttcacgggc tttgaggccg gagatgcgca ggatgccaac gccgccacgt
     1321 cccggaggcg tggcctgggc tacgatagtg tcattatcgc tcatgatgtt cctgttgctt
     1381 tgtgtggcgg atgtgtggtg cttatccgcc ctacgaaaag aaaaaaggcg gtcaactgac
     1441 cgcccttatt ttagcgaaaa ctcaccgaat caggattttt tcttctcgcg gctatgcaag
     1501 ccacgttttt ccagaccacg gtaaatcagc tgctgctgaa taatggttac caggttgctg
     1561 acgatatagt acagcaccag acctgacggg aaccacagga agaacacagt gaagatgacc
     1621 ggcataaagg tcatgatctt ctgctgcatc gggtcggtca cagtggtcgg cgacatcttc
     1681 tgaatgaaga acatcgttac gcccatcagg atcggcagga tgtagtacgg gtcctgtgca
     1741 gacaggtcgt ggatccacag tgcaaacggt gcctgacgta gttcaacgga acccatcagc
     1801 atgtagtaca acgccaggaa gattggcatc tggatcagca gcgggaagca gccgcccagc
     1861 gggttaacct tctcagcttt gtacagcgcc atcatttcct ggcttatgcg ctgtttgtca
     1921 tcgcccagac gctcacgcat tgcctgaatc ttcggctgca gcatacgcat cttcgccatg
     1981 gaggtgtact gcgctttggt cagcgggtac atgatgccac gaacgataaa ggtgatgatg
     2041 ataatggaga agccccagtt acccacaaag ctatggatcc atttcagcag tttgaacagt
     2101 ggctgagaga tgaaccacaa ccaaccgtaa tcaacggtca gatccaggtg cggagcaaca
     2161 gctgccattt tgtcctgaat ttccgggcca acccacaggg tgctgttcat cgcgccagtc
     2221 tgaccaggct gaaccagtac cggctgagat ttatagccga tagcggcgat gccgttaccc
     2281 agattagcgg tgtagaagtt gttggtaccg tcgttatgcg ggatccacgc cgtcgcgaaa
     2341 tactgttgca gcattgccac ccaaccacct ttcgaagaga tgttcaggtt ttcgttatcg
     2401 gcaatggtat cgaatttgta tttctcgtac ttctcgtcag gcgtggagta cgccgcacca
     2461 cggaaggtgt gcagtgcgaa gttgctgctt ccggtatcga gatgcggtgg cagagtgatg
     2521 gattgcttca actgaccaaa ggtggagatt tccagcggtt tctcgccagc gttctgcacg
     2581 ttgtagttga cgttgacagc gtaatcacca cgtttcagga caaacgtttt ggtaaacgtg
     2641 ttgcctgccg cgtcggtata cgtcatcggc acctgcagtt cgttttgacc ttcagccagc
     2701 acataagcgt ctttttcaac gttatatagc ggacgcgggc cgttagccgg gttatccggg
     2761 ccatcacgac cggtcagacc gctctgtgcc tgataaataa actgcggtga agtttccagc
     2821 agctggaacg gctgggtaga gttcagctct ttcgggtaag ccggcagcag ggcctgctca
     2881 acatcaccac cacgggtgtt gatggtcaga tcaagcacgt cggtcttaac cgagatcagt
     2941 ttcccctggc cactggccgg tacgccctgg tcggcggcgc tacccgctgc ggtggtcgtt
     3001 gtctgcgtgg tctgttgggc ctgaggttgc gggtttttat cctgctccca ggcttgccag
     3061 atcatgaaag acacgaacag caaagcgatg actaaaagat tgcgttgcga atccatcgtt
     3121 agtgttctct ggtatcaaat ggtccgggcg ggacgggatc gtcaccacca gggtgtaaag
     3181 ggtggcattt taatacgcgt ttcaccgtca accaactgcc ttttatcact ccaaacctgc
     3241 gcaatgcctc aattccgtag cttgaacagg ttggagtgaa acgacaatgc ggcccgagta
     3301 gcggactaat caggcgttga tagacccgaa tgagggctat caggacccgc gagccaggcg
     3361 acagtggcgg cgccataatt tttccaacgc ttccgagaga gcacggttat cgaggtcggc
     3421 aacccctttt ttcgccacca ccacgaaatc catagccggg agttcatgtt ggcgcagacg
     3481 gaagctttca cgcgtcagac gtttaatccg attgcgttca tgggcgcgtc gaacgttttt
     3541 cttggcgact gtaagaccga tacggggatg ccccagcgaa ttcaggcggc cgagaatagt
     3601 aatttgcggc gtgccagccc gttgtggctg ctggaagacg aatgtgaatt gactgggagt
     3661 taacaagcgt aactccctgg gaaatgcgag cttaaccact caggggttag ctttattact
     3721 tagaaacggt cagacgagcg cggcctttag cacgacgacg tgccagaacc tgacgaccat
     3781 ttttagtagc catacgagca cggaagccgt gagaacggtt gcgcttcagt acagacggtt
     3841 gaaaagtgcg tttcatggcg atttctacct aaacttgaat aaattcaatg gctttattgg
     3901 atatccgccg aaaaatgaaa cgatggacac cgaagccatg ggtgattaaa gaggccggat
     3961 tgtaataatt gtacactccg gagtcaattc tctttcctta tttaccgcgc ttttccgcac
     4021 cttttcgcgg ggaaaacgta cggcctcaca ccagtgaaaa ccagcatggc gcgccgggtg
     4081 gaggattata cgggctgacg ggtaaagcgc aaggatcgtc ctggatcttt attagatcga
     4141 ttaagccatt ttttgtctat ggtcattaaa ttttccaata tgcggcgtaa atcgtgcccg
     4201 gcctgcggca ggatcgttta cacttagcga gttctggatc atcctgtgga taaatcggga
     4261 aaatctgtga gaaacagaag atctcttgcg cagtttaggc tatgatccgc ggtcccgatc
     4321 gttttgcagg atcttgatcg ggcatataac cgcagacagc ggttcgtgcg tcaccctcat
     4381 gcagggtctt ttcgacgtac gtcaacaatc atgaatgttt cagccttagt cattatcgac
     4441 ttttgttcga gtggagtccg ccgtgtcact ttcgctttgg cagcagtgtc ttgcccgatt
     4501 gcaggatgag ttaccagcca cagaattcag tatgtggata cgcccattgc aggcggaact
     4561 gagcgataac acgctggccc tgtacgcgcc aaaccgtttt gtcctcgatt gggtacggga
     4621 caagtacctt aataatatca atggactgct aaccagtttc tgcggagcgg atgccccaca
     4681 gctgcgtttt gaagtcggca ccaaaccggt gacgcaaacg ccacaagcgg cagtgacgag
     4741 caacgtcgcg gcccctgcac aggtggcgca aacgcagccg caacgtgctg cgccttctac
     4801 gcgctcgggt tgggataacg tcccggctcc ggcagaaccg acctatcgtt ctaacgtaaa
     4861 cgtcaaacac acgtttgata acttcgttga aggtaaatct aaccaactgg cgcgcgcggc
     4921 ggctcgccag gtggcagata accctggcgg tgcttataac ccgttgttcc tttatggcgg
     4981 cacgggtttg ggtaaaactc acctgctgca tgcggtgggt aacggcatta tggcgcgcaa
     5041 gccgaatgcc aaagtggttt atatgcactc cgagcgcttt gttcaggaca tggttaaagc
     5101 cctgcaaaac aacgcgatcg aagagtttaa acgctactac cgttccgtag atgcactgct
     5161 gatcgacgat attcagtttt ttgctaataa agaacgatct caggaagagt ttttccacac
     5221 cttcaacgcc ctgctggaag gtaatcaaca gatcattctc acctccgatc gctatccgaa
     5281 agagatcaac ggcgttgagg atcgtttgaa atcccgcttc ggctggggac tgactgtggc
     5341 gatcgaaccg ccagagctgg aaacccgcgt ggcgatcctg atgaaaaagg ccgacgaaaa
     5401 cgacattcgt ttgccgggcg aagtggcgtt ctttatcgcc aagcgtctac gatctaacgt
     5461 acgtgagttg gaaggcgcgc tgaaccgcgt cattgccaac gccaacttta ccggacgggc
     5521 gatcaccatc gacttcgtgc gtgaggcgct gcgcgacttg ctggcattgc aggaaaaact
     5581 ggtcaccatc gacaatattc agaagacggt ggcggagtac tacaagatca aagtcgcgga
     5641 tctcctttcc aagcgtcgat cccgctcggt ggcccgtccg cgccagatgg cgatggcgct
     5701 ggcgaaagag ctgactaacc acagtctgcc ggagattggc gatgcctttg gtggtcgtga
     5761 ccacacgacg gtgcttcatg cctgccggaa gatcgagcag ttgcgtgaag agagccacga
     5821 tatcaaagaa gatttttcta atttaatcag aacattgtca tcgtaaacct atgaaattta
     5881 ccgtagaacg tgagcattta ttaaaaccgc tacaacaggt gagcggtccg ttaggtggtc
     5941 gtcctacgct accgattctc ggtaatctgc tgttacaggt tgctgacggt acgttgtcgc
     6001 tgaccggtac tgatctcgag atggaaatgg tggcacgcgt tgcgctggtt cagccgcacg
     6061 aaccaggggc gacgaccgtt ccggcgcgca aattctttga tatctgccgt ggtctgcctg
     6121 aaggcgcgga aattgccgtg cagctggaag gtgagcggat gctggtgcgc tccgggcgta
     6181 gccgtttttc gctgtctacc ctgccagcgg cagatttccc gaacctcgat gactggcaga
     6241 gcgaagtcga atttaccctg ccgcaggcga cgatgaagcg tctgattgaa gcgacccagt
     6301 tttctatggc acatcaggac gttcgctatt acttaaacgg tatgctgttt gaaaccgaag
     6361 gtgaagaact gcgcaccgtg gcgaccgacg gccaccgtct ggcagtctgt tcaatgccaa
     6421 ttggtcaatc tttgccaagc cattcggtga tcgtgccgcg taaaggcgta attgaactga
     6481 tgcgtatgct cgacggcggc gacaatccgc tgcgcgtgca gattggcagc aacaacattc
     6541 gcgcccacgt tggcgacttt atcttcacct cgaaactggt ggatggtcgc ttcccggatt
     6601 accgtcgcgt tctgccgaag aacccggaca agcatctgga agctggctgt gatctgctca
     6661 agcaggcgtt tgcccgtgcg gcaattctct ctaacgagaa attccgcggc gtgcgtctgt
     6721 atgtcagcga aaaccagctg aaaatcaccg ccaacaaccc agaacaggaa gaagcggaag
     6781 agatcctcga cgttacctat agcggtgcgg agatggaaat cggcttcaac gtcagctatg
     6841 tgctggatgt tctgaacgca ctgaaatgcg aaaacgtccg catgatgctg accgattcgg
     6901 tttccagcgt gcagattgaa gatgcggcca gccagagcgc ggcttatgtt gtcatgccaa
     6961 tgagactgta atgtccctca cccgcttgtt gatccgcgat ttccgcaaca ttgaaaccgc
     7021 ggatctcgcc ttatctcccg gctttaactt tctggtaggt gccaacggca gtggcaaaac
     7081 cagcgtgctg gaagccatct atacgctcgg ccacggtcgg gcgtttcgca gtttgcagat
     7141 tggtcgcgtc attcgccacg agcaggaggc gtttgttctc cacggacgat tacagggcga
     7201 agagcgtgag acggcgattg gtttaaccaa agacaaacaa ggcgacagca aagtccgtat
     7261 tgacggtaca gacgggcata aagtcgcgga gctggcgcac ctgatgccaa tgcagctgat
     7321 aacgccagaa gggtttactt tactcaatgg cggccccaaa tacagaagag cattcctcga
     7381 ctggggatgc tttcacaacg aacccggatt tttcaccgcc tggagcaatc tcaagcgatt
     7441 gctcaagcag cgcaatgcgg cgctgcgcca ggtgactcgt tacgaacagc tacgcccgtg
     7501 ggataaagaa ctgatcccgc tggcggagca aatcagcacc tggcgcgcgg agtatagcgc
     7561 cggtatcgca gccgatatgg ctgatacctg taagcaattt ctccctgagt tttctctgac
     7621 tttctctttc cagcgcggct gggagaaaga gacagagtac gctgaggtgc tggaacgtaa
     7681 ttttgaacgc gatcgccagt taacctacac cgcgcacggc ccgcataaag cggacttacg
     7741 cattcgcgcc gacggtgcgc cggtggaaga taccttatcg cgtggacagc ttaagctgtt
     7801 gatgtgcgcc ttacgtctgg cgcaaggaga gttcctcacc cgtgaaagcg ggcggcggtg
     7861 tctctacctg atagatgatt ttgcctctga gcttgatgat gagcgtcgcg ggctgcttgc
     7921 cagccgctta aaagcgacgc aatcacaggt ctttgtcagc gcgatcagtg ctgaacacgt
     7981 aatagacatg tcggacgaaa attcgaagat gtttaccgtg gaaaagggta aaataacgga
     8041 ttaacccaag aataaatgag cgagaaacgt tgatgtcgaa ttcttatgac tcctccagta
     8101 tcaaagtcct gaaagggctg gatgcggtgc gtaagcgccc gggtatgtat atcggcgaca
     8161 cggatgacgg caccggtctg caccacatgg tattcgaggt ggtagataac gctatcgacg
     8221 aagcgctcgc gggtcactgt aaagaaatta tcgtcaccat tcacgccgat aactctgtct
     8281 ctgtacagga tgacgggcgc ggcattccga ccggtattca cccggaagag ggcgtatcgg
     8341 cggcggaagt gatcatgacc gttctgcacg caggcggtaa atttgacgat aactcctata
     8401 aagtgtccgg cggtctgcac ggcgttggtg tttcggtagt aaacgccctg tcgcaaaaac
     8461 tggagctggt tatccagcgc gagggtaaaa ttcaccgtca gatctacgaa cacggtgtac
     8521 cgcaggcccc gctggcggtt accggcgaga ctgaaaaaac cggcaccatg gtgcgtttct
     8581 ggccaagcct tgaaaccttc accaatgtga ccgagttcga atatgaaatt ctggcgaaac
     8641 gtctgcgtga gttgtcgttc ctcaactccg gcgtttccat tcgtctgcgc gataagcgcg
     8701 acggcaaaga agaccacttc cactatgaag gcggcatcaa ggcgttcgtt gaatatctga
     8761 acaagaacaa aacgccgatc cacccgaata tcttctactt ttccaccgaa aaagacggta
     8821 ttggcgtcga agtggcgttg cagtggaacg atggcttcca ggaaaacatc tactgcttta
     8881 ccaacaacat tccgcagcgt gacggcggta ctcacctggc aggtttccgt gcggcgatga
     8941 cccgtactct gaacgcctac atggacaaag aaggctacag caaaaaagcc aaagtcagcg
     9001 ccaccggtga cgatgcgcgt gaaggcctga ttgcggtcgt ttccgtgaaa gtgccggacc
     9061 cgaaattctc ctcacagacc aaagacaaac tggtttcttc tgaggtgaaa tcggcggttg
     9121 aacagcagat gaacgaactg ctggcggaat acctgctgga aaacccaacc gacgcgaaaa
     9181 tcgtggttgg caaaattatc gatgctgccc gtgcccgtga agcggcgcgt cgcgcgcgtg
     9241 aaatgactcg ccgtaaaggt gcgctcgact tagcgggcct gccgggcaaa ctggcagact
     9301 gccaggaacg cgacccagcg ctttccgaac tatacctagt ggaaggggac tccgcgggcg
     9361 gctctgcgaa gcaggggcgt aaccgcaaga accaggcgat tctgccgctg aagggtaaaa
     9421 tcctcaacgt cgagaaagcg cgcttcgata agatgctctc ttctcaggaa gtggcgacgc
     9481 ttatcaccgc gcttggttgt ggtatcggtc gtgacgagta caacccggac aaactgcgtt
     9541 atcacagcat catcatcatg accgatgcgg acgtcgacgg ctcgcacatt cgtacgctgc
     9601 tgttgacctt cttctatcgt cagatgccgg aaatcgttga acgtggtcac gtctacatcg
     9661 ctcagccgcc gctgtacaaa gtgaagaaag gtaagcagga acagtacatt aaagacgacg
     9721 aagcgatgga tcagtaccag atctctatcg cgctggatgg cgcaacgctg cacaccaacg
     9781 ccagtgcacc ggcgctggct ggcgaagcgt tagagaaact ggtgtctgag tacaacgcga
     9841 cgcagaaaat gatcaaccgc atggagcgtc gttatccgaa agcaatgctg aaagagctta
     9901 tctatcagcc gacgctgacg gaaaccgacc tttctgacga gcaaaccgtt acccgctggg
     9961 tgaacgcgct ggtcagcgaa ctgaacgata aagaacagca cggcagccag tggaagtttg
    10021 atgtccatac caatgccgaa caaaacctgt tcgagccgat tgttcgcgtg cgtacccacg
    10081 gtgtggatac tgactatccg ctggatcacg aatttatcac tggcggcgaa tatcgtcgta
    10141 tctgcacgct gggtgagaaa ctgcgtggct tgctggaaga agatgcattt atcgaacgtg
    10201 gcgaacgtcg tcagccggta gccagcttcg agcaggcgct ggactggctg gtgaaagagt
    10261 cccgtcgcgg cctctccatc cagcgttata aaggtctggg cgagatgaac ccggaacagc
    10321 tgtgggaaac caccatggac ccggaaagcc gtcgtatgct gcgcgttacc gttaaagatg
    10381 cgattgctgc cgaccagttg ttcactacgc ttatgggcga cgccgttgaa ccgcgccgtg
    10441 cgtttatcga agagaacgct ctgaaagcgg cgaatatcga tatttaatgg ctttttgttg
    10501 ctgataaaat gacttagtcg tcagtccggg cggagatcat ctccgccctt tttattcctg
    10561 caatccgcaa ttatcatccc gcagatctat actttttggg tcaacacgta acgctttaca
    10621 ggtgattatc atggggcttt ttgatgaagt tgtcggtgcc tttctgaaag gcgatgcggg
    10681 gaaatatcag gcgattttga gctgggttga ggagcagggc ggcattcagg tgctgctgga
    10741 aaaactgcaa agtggcggct taggggccat tctctcaacc tggctgagta atcaacagag
    10801 caatcaatca gttagcggcg agcaagtgga atcggcgctc ggcacaaatg cggtgtccga
    10861 tctcgggcaa aaacttggcg tggataccag tacagcttcc agtttactgg cagaacaatt
    10921 gccgaagatt attgatgcac tctcaccgca aggtgaagtg tcaccacaag ccaataacga
    10981 tctgctttcc gcaggtatgg aactgctgaa agggaaactc ttccgctaag caaatgggga
    11041 gcacgcaatg cgcgttgcat ccccttttcc ctcgggtgtt gtactgattt ttgagcggaa
    11101 tcgcgttagc atgggtcagg aaccaatcta cctggggaac tcatggctat caaactcatt
    11161 gctatcgata tggatggcac ccttctgctg cccgatcaca ccatttcacc cgccgttaaa
    11221 aatgcgattg ccgcagctcg cgcccgtggc gtgaatgtcg tgctaacgac gggtcgccct
    11281 tatgcaggtg ttcacaatta cctgaaagag ctgcatatgg aacagccggg cgactactgc
    11341 attacttata acggcgcgct ggtacagaag gccgctgatg gtagcaccgt ggcgcaaacc
    11401 gctctcagct atgacgacta ccgtttcctg gaaaaactct ctcgcgaagt cggttctcat
    11461 ttccacgccc tggaccgcac cacgctgtac accgccaacc gagatatcag ctactacacg
    11521 gtgcatgaat ctttcgttgc caccattccg ctggtgttct gcgaagcgga gaaaatggac
    11581 cccaataccc agttcctgaa agtgatgatg attgatgaac ccgccatcct cgaccaggcc
    11641 attgcgcgta ttccgcagga agtgaaagaa aaatacaccg tgctgaaaag tgcgccgtac
    11701 ttcctcgaaa tcctcgataa acgcgttaac aaaggtacgg gtgtgaaatc actggccgac
    11761 gtgttaggta ttaaaccgga agaaatcatg gcgattggcg atcaggaaaa cgacatcgca
    11821 atgattgaat atgcaggcgt cggtgtggcg atggataacg ctattccttc ggtgaaagaa
    11881 gtggcgaact ttgtcaccaa atctaacctt gaagatggcg tggcatttgc tattgagaag
    11941 tatgtgctga attaatcagt gaacgggcaa acggcatgaa tgcgtttgcc cgcagcgatt
    12001 aacaatttct ggtcgtgcat aaaatcagga actggagtgg tgacgttact atatccagcg
    12061 taagagtgac tggcagcagc gcattaacga ggccgcgcgg gacgccatct gttgctttgt
    12121 actcataaaa tcccaccgta aagggttgat ggaaaaccat cacgtttgag taatccgctt
    12181 ttttattttt ctggtggatt gagcctttca aaccgtccag catccatgta caacgccggg
    12241 tatcacaaat aaaaccatat tgtgttgcca gtgccttatc ttcttcgttg ttccagtcgt
    12301 aatagagcgt aatagcacca gagaaattct tattactgct gcttaaaata aatttcgcgt
    12361 tatcagaaac ggtgattctt tcaccatgga ttgccggatc gttcagcatg ttaaccacct
    12421 catcgccgcc ggtggtcaat agatagtcca acgattcacc aacaaaaaca taacctttcg
    12481 taccgttact gtcttcagcc agcgataaac ccttaatcgt gtctgaccgc cattgctcgg
    12541 catgtggacg gcttgcagta ttggaaacca atccccaggt gatacatccg gtcaacgcaa
    12601 atgccaaaga acacaaaccg gcagccttaa aaaatccttt aaaattcaat ttcatgatta
    12661 atcctttaaa caagagtgct aagccgttga aagcatactc ttctttccct cctgtttagc
    12721 gcctgatctc ccttttcaga tttttgcccg acgtagaaca ttttcttatg gattgttctt
    12781 tttgtgatct aaattgtagt acaacaatat aaatttgtac tacattatgc gcacagcaaa
    12841 cgcgaacgtc atcacgctgg tactacaaaa ttgccgcgtt atgcatcgat cggggtaaag
    12901 tagagaggaa catacagagc acaaggactc tccatgactc tcaataaaac cgatcgcatc
    12961 gttattacgc tgggtaaaca gatcgttcac ggcaaatacg tgccaggctc gccgcttccg
    13021 gctgaggcag aactctgtga ggagtttgca acctcgcgca acatcatccg tgaggtattt
    13081 cgttcgctga tggcgaagcg gctgattgaa atgaaacgtt atcgcggggc gtttgtggct
    13141 ccgcgtaacc agtggaatta cctcgacact gacgtactgc aatgggtgct ggaaaacgac
    13201 tatgacccac ggcttatcag tgcaatgagc gaagtgcgaa acctggtaga gccggctatt
    13261 gcccgttggg cagcagagcg cgcgacctcc agcgatctgg cgcagattga atcggcgctg
    13321 aacgagatga ttgctaacaa tcaggaccgc gaagcgttta acgaagcgga tattcgctac
    13381 cacgaggcgg tgctgcagtc ggtgcataac ccggtgttac aacaactgag cattgcgatc
    13441 agttcgctgc agcgggcggt ttttgaacga acctggatgg gcgatgaggc caacatgccg
    13501 caaacgctcc aggaacataa ggcgctgttc gatgcgataa ggcatcagga cggcgatgcg
    13561 gcagagcagg cggcgcttac catgatcgcc agctcgacac gaaggttaaa ggaaatcaca
    13621 tgacagctcg ctacatcgca attgactggg gatcgaccaa tctgcgcgcc tggctttatc
    13681 agggcgacca ctgcctggac agcaggcaat cagaagcagg cgtcacgcgc ctgaatggaa
    13741 aatctccggc tgcggtgtta gcagaagtga cgaccgactg gcgtgaagag aatacgccag
    13801 tggtaatggc aggaatggtc ggcagcaatg tcggctggaa agttgcgccg tatttatcca
    13861 ttcctgcccg tttttcgtct attggcgaac aattaacgtc tgttggcgac aatatctgga
    13921 ttattcccgg attatgcgtc tctcatgacg ataaccacaa tgtgatgcgt ggcgaagaaa
    13981 cacaattgat cggcgcgcgc actctcgctc cttcctctct ttatgtcatg cccggtacgc
    14041 attgcaaatg ggtgcaggcc gatagccagc aaattaacga ttttcgcacc gtgatgaccg
    14101 gtgaattaca tcatttactg ttaaatcact cattgattgg cgcaggtttg ccgccgcagg
    14161 aaaacgctgt cgatgctttc gcggctggtc ttgagcgtgg ccttaatgcg cccgccgtat
    14221 tgccacagct ttttgaagtt cgcgcctcgc atgtgctggg aacacttccc cgcgaacagg
    14281 tcagcgaatt tctctctggt ttgttgattg gcgcagaggt cgccagtatg cgcgactatg
    14341 tggcccatca acaagtcatc acccttgtcg ccggaacatc gctgaccgcg cgctaccagc
    14401 aagcctttca ggcgatgggt tgtgacgtga cggcggtggc gggcgacacg gcatttcagg
    14461 ctggtataag gaacatcgct catgcagtgg caaactaaac tcccgctgat cgccattttg
    14521 cgcggcatta cgcccgacga ggcgctggcg catgttggcg cggtgattga cgccgggttc
    14581 gacgcggttg aaatcccgct gaattctcca caatgggagc agagcattcc cgccatcgtt
    14641 gatgcgtatg gcgacaaggc gttgattggc gcaggtacgg tactgaaacc tgaacaggtc
    14701 gatgcgctcg ccagaatggg ctgtcagctc atcgttacgc ccaatatcca tagtgaagtg
    14761 atccgccgtg cggtgggcta cggcatgacc gtctgccccg gctgcgcgac ggcgaccgaa
    14821 gcctttaccg cgctcgacgc gggtgcgcag gcactgaaaa tatttccgtc atcggctttt
    14881 ggtccgcaat acatcaaagc attaaaagcg gtattaccac cgaacatcgc ggtctttgcc
    14941 gttggcggtg tgacgccaga aaacctggcg cagtggatag atgcaggctg tgccggagcg
    15001 ggcttaggta gcgatctcta tcgcgccggg cagtccgtag aacggactgc gcagcaggca
    15061 gcagcatttg ttaaggcgta tcgagaggca gtgcaatgaa aatcaccaaa attaccacgt
    15121 atcgtttacc tccccgctgg atgttcctga aaattgaaac cgatgaaggc gtggtcggtt
    15181 ggggcgagcc cgtgattgaa ggtcgcgctc gcacggtgga agcggcagtc cacgagctga
    15241 gtgactattt gattggtcag gacccgtcac gcatcaacga cttatggcaa gtgatgtatc
    15301 gcgccggatt ttatcgtggc ggtccgattc tgatgagtgc catcgccggg atcgaccagg
    15361 ctttatggga tatcaaaggc aaagtgctga atgcgccggt ctggcaactg atgggcggcc
    15421 tggttcgcga caaaattaaa gcctacagtt gggtcggcgg cgatcgtccg gcggatgtta
    15481 tcgacggcat taaaacgcta cgcgaaatcg gcttcgatac cttcaaactg aacggttgtg
    15541 aagaactggg gctaattgat aactcccgcg cggtagatgc ggcagtcaac accgtggcac
    15601 aaattcgtga agcttttggc aatcagattg agtttggtct ggacttccat ggtcgcgtca
    15661 gcgcgccgat ggcgaaagtg ctgattaaag agctggaacc gtatcgtccg ctgtttattg
    15721 aagagccggt gctggcggaa caggccgaat actacccgaa actggcggca caaacgcata
    15781 ttccactggc ggcgggtgag cgcatgttct cacgcttcga ttttaaacgt gtgctggagg
    15841 caggcggtat ttcgattctg caaccggatc tctcccacgc aggcggtatt accgaatgct
    15901 acaaaattgc cggaatggca gaagcctatg acgtgaccct tgcgccgcac tgtccgctcg
    15961 gaccgattgc actggcggct tgcctgcata tcgactttgt ttcctataac gccgtccttc
    16021 aggaacaaag tatgggcatt cattacaaca aaggcgcgga gttactcgac tttgtgaaaa
    16081 acaaagagga cttcagcatg gtcggcggtt tctttaaacc attaacgaaa ccgggcttag
    16141 gcgtggaaat cgacgaagct aaagttattg agttcagtaa aaatgccccg gactggcgta
    16201 atccgctctg gcgtcatgaa gataacagcg tagcagagtg gtaattcctg ccacgtaagc
    16261 ccctcatcgg gcactaaaac agcaatacaa aaatataacc ctctgtaaat tacagtgcat
    16321 ggtgagcggc ttcgctatgc ccaaaatcag gagaaagctc gctatggata ttcccgttaa
    16381 tgcagcaaag ccggggcgtc ggcgttatct gacgctggtg atgatcttta ttacggtggt
    16441 catttgttat gttgaccgtg ctaacctggc cgtggcttct gcccatattc aggaagagtt
    16501 tggtattact aaagcgcaaa tgggctatgt attttcggcc ttcgcctggc tttatacgct
    16561 atgtcagatc cccggcggtt ggtttttaga tcgcgtaggt tcacgcgtga cttattttat
    16621 tgcgatattt ggctggtcag tggcgacgtt attccagggc tttgccacgg gcttaatgtc
    16681 attaattggt ctgcgcgcga taaccggtat tttcgaagcg cccgcgttcc cgaccaataa
    16741 ccggatggta accagttggt tcccggaaca tgaacgcgct tctgccgttg gtttttatac
    16801 gtctggtcag tttgtcggtc tggcgtttct gactccgctg ctgatctgga ttcaggagat
    16861 gttgagctgg cactgggtgt tcattgtcac cggtggtatc ggcattatct ggtcgctgat
    16921 ttggtttaag gtttatcagc cgccgcgtct gaccaaaggc atcagcaaag ctgaactgga
    16981 ttacattcgt gatggcggcg gtctggtgga tggcgatgcg ccggtgaaaa aagaggcgcg
    17041 tcagccgtta acagccaaag actggaaact ggtgttccat cgtaaactga tcggtgtcta
    17101 tcttgggcaa tttgcggtgg cttctacact gtggtttttc ttaacctggt tcccgaacta
    17161 tttaacccag gaaaaaggaa tcacggcgct gaaagcaggt tttatgacca cggtgccatt
    17221 cctcgcggcg tttattggcg tcctgctctc tggctgggtc gcggatctgc tggtacgtaa
    17281 gggcttttca ctgggctttg cgcgtaaaac gccgattatc tgcggcttgc tgatctccac
    17341 ctgcattatg ggtgcgaact acaccaacga tccgatgatg attatgtgcc tgatggcgct
    17401 ggcattcttc ggcaacggtt ttgcttcgat aacctggtcg ctggtctctt ctctggcacc
    17461 gatgcgcctg attggtttaa ccggcggcgt gtttaacttt gccggtggtc tgggcggcat
    17521 caccgttccg ctggtggtgg ggtacctggc gcagggttac ggtttcgcgc ctgcactggt
    17581 ttatatctcc gccgtcgcgt tgattggcgc gctctcttac atcctgctgg tgggcgatgt
    17641 gaagcgcgtt ggataatctt tcaactgtgg aatatgtgcc actccactgc gcataatcca
    17701 ctttcttaaa gccggtgcgg tcaggcatcg gctttttact atcttgccga agagttttaa
    17761 acgcagtttg cgagtggcgc gccagtaagc ggtattgagc ttctctggct gcttcagtaa
    17821 taccgaacgc agaatctctg cgctatccag cgcatagcta atcccctcca gcgagctggc
    17881 gctgataaat cccgccgctt cgccaatcag aaaggcgttg tccttgccgc agacaaaatc
    17941 ctgccagcgt gagggaaaca gcaccgtgca tttttcgctt ttcaccgcct taccaaactg
    18001 gaactgaaag gcgctcattt tctcttttag cgtcgtgaaa cgcgtctgac cgtctttcat
    18061 gggataggca ccgccaaaga taaaataacc gtctttgctg atactccatg agtaacagtc
    18121 agttatcgca ttatcaaaga tgcaggagta gaacggtacc ggatgtttct ctgcgaacca
    18181 ctgctggatg gcgacatatt tacgaatctg atgatccggg tagagatgtc gccgcaccat
    18241 cgagttggca ccatcggcac cgaccagata gcgggcggta atatgctgct cccagccgtc
    18301 tgcgcgaaaa atgacatgcc atttatcatc ctcacgccag atttttcgac acaggctgtc
    18361 gtggtaaacc tcaacgctgg cggggatcag cgatttcatc cacaagtcga aagcatggcg
    18421 attaatattg atatagcttc gctggtagtt acgcgtgagc gatgcggcga cgtcgacggt
    18481 tttgacgctg aaaatctgcg ggttggcgat gacatcgaca ggaagcgtca gtccatcgcg
    18541 aataaaagat cgctgcgcgt ccggtgccag aagaccgcca caaggtttgc tgaaaccttc
    18601 agtaccacac tggtgctttt tatccagcgc gatcacctgc attttgcctg ctaactttcg
    18661 cgccaacgcc gaccctgccg ggccgaggcc aataatcgcc acgtcgaaat gttccatctt
    18721 ttcactcaca ttcatcacga tggcaaaaga ctattgtctg aaagtgaagt ccttgtgaag
    18781 tatgatggcg ggaaccgttt tgtatcagga ccgcgccatg aaacagataa cctttgctcc
    18841 ccgtaatcac ctgctcacca ataccaatac ctggacgccc gacagccagt ggctggtgtt
    18901 tgacgtgcgg ccttctgggg cgtcgtttac cggcgagacc attgagcgtg tgaatatcca
    18961 taccggcgag gtcgaggtta tctatcgcgc gtcacagggc gcatacgtcg gcgtggtgac
    19021 cgttcatccg aagtcagaga agtatgtctt catccacggc ccggaaaatc ctgatgaaac
    19081 atggtattac gactttcatc atcggcgcgg agtgattgtt gaaagcggca aggtgagcaa
    19141 tctcgatgca atggatatta ccgcgccata tacgccagga gcgctgcgcg gcggcagtca
    19201 tgtacatgtc tttagcccaa acggtgaaag ggtgagcttt acctacaacg accatgtaat
    19261 gcatgaactc gatccggcgc tggatttgcg aaatgtcggt gttgctgcac cgtttggccc
    19321 ggtcaacgta caaaagcagc atccgcgtga atacagcggt agccactggt gcgtgctggt
    19381 aagtaaaacc acgcccacgc cacagcctgg cagtgatgaa atcaatcgtg cttatgaaga
    19441 aggatgggta ggaaatcacg cgctggcgtt tattggcgac acactttcgc caaagggcga
    19501 gaaagtgccg gagctgttta ttgtcgagtt gccgcaagat gaagctggct ggaaagtggc
    19561 aggtgatgcg ccgttaagtg gaacggaaac aaccctgccc gcgccaccgc gtggtgtcgt
    19621 gcagcgacgt ttaaccttta cgcaccatcg ggcttctccg ggattagtca acgtcccgcg
    19681 ccactgggtg cgctgtaatc cgcagggtac gcaaatcgcg tttttaatgc gtgatgataa
    19741 cggcattgtg caactgtggc ttatctcgcc acagggcggt gaaccacgcc agttaaccca
    19801 taataaaacg gatattcagt ctgcatttaa ctggcatcca tcaggcgaat ggctgggatt
    19861 tgtgctggat aatcggattg cttgtgccca tgcgcaaagc ggcgaggttg agtatttaac
    19921 cgaaaaccac gccaatccac cttctgcgga cgccgtggtt ttctcgccgg atggtcaatg
    19981 gttggcgtgg atggaggacg gtcagttgtg gatcaccgaa actgaccgct agctctcagt
    20041 ttgcaggcat tttggcgggg ggaatgacgt tattggtttc ttgcgcgtcg gcttcgcttt
    20101 tttcaacgcg cgagcgcact gagctgtctt tgcggaacac atcccacggt agcaatattg
    20161 tgtccagtac tgcggtaaat ggcatgtcga gtatcgccag cgattttgtg ccccagttgg
    20221 tttcatcgtc gccaatcatg gttgcgctgg cgcgggtgcc aggatacgtt ccttctttcc
    20281 ccccggtatg ggacatcacg ctggagcatc ctccgagtaa tgtcattccg ctgcatatca
    20341 tgaacgctaa caacacattt tttatcataa taaattcatc tgttgatcgt gggtgttggc
    20401 ctgatgagtt atagcgatcc cttgctgaaa ataacatcat cattacgtcg cactgtggcg
    20461 gctatcgcac tttaacgttt cgtgctgccc cctcagtcta tgcaatagac cataaactgc
    20521 aaaaaaaagt ccgctgataa ggcttgaaaa gttcatttcc agacccattt ttacatcgta
    20581 gccgatgagg acgcgcctga tgggtgttct ggctacctga cctgtccatt gtggaaggtc
    20641 ttagattctc gctgatttca ggagctattg attatgcgta actttgattt atccccgctt
    20701 taccgttctg ctattggatt tgaccgtttg tttaaccact tagaaaacaa ccagagccag
    20761 agtaatggcg gctaccctcc gtataacgtt gaactggtag acgaaaacca ttaccgcatt
    20821 gctatcgctg tggctggttt tgctgagagc gaactggaaa ttaccgccca ggataatctg
    20881 ctggtggtga aaggtgctca cgccgacgaa caaaaagagc gcacctatct gtaccagggc
    20941 atcgctgaac gcaactttga acgtaaattc cagttagctg agaacattca tgttcgtggt
    21001 gctaacctgg tgaatggtct gctgtatatc gatctcgaac gcgtgattcc ggaagcgaaa
    21061 aaaccgcgcc gtatcgaaat caactaattc cctaaggccg cctggcgcgg cctgacatct
    21121 ccatgctcgc cgtcagggag catatgcgaa tcttcggatt tgcaggtact tactcgcttc
    21181 ttagaaggag aaatgactat gcgtaacttc gatttatccc cactgatgcg tcaatggatc
    21241 ggttttgaca aactggccaa cgcactgcaa aacgccggtg aaagccagag cttcccgccg
    21301 tacaacattg agaaaagcga cgataaccac taccgcatta ccctcgcgct ggcaggtttt
    21361 cgtcaggaag atttagagat tcaactggaa ggtacgcgcc tgagcgtaaa aggcacaccg
    21421 gagcagccaa aagaagagaa aaaatggctg catcaagggc ttatgaatca gccttttagc
    21481 ctga
//
LOCUS       DAOFSV010000070        21462 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.070, whole genome shotgun sequence.
ACCESSION   DAOFSV010000070 DAOFSV010000000
VERSION     DAOFSV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.070"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            219..1517
                     /gene="kgtP"
                     /locus_tag="QBH27_002747"
     CDS             219..1517
                     /gene="kgtP"
                     /locus_tag="QBH27_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDQ7015042.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALRLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAFANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1514..1837)
                     /locus_tag="QBH27_002748"
     CDS             complement(1514..1837)
                     /locus_tag="QBH27_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDQ7015043.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1883..3238)
                     /gene="pssA"
                     /locus_tag="QBH27_002749"
     CDS             complement(1883..3238)
                     /gene="pssA"
                     /locus_tag="QBH27_002749"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDQ7015044.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVNVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3352..6012)
                     /gene="pat"
                     /locus_tag="QBH27_002750"
     CDS             complement(3352..6012)
                     /gene="pat"
                     /locus_tag="QBH27_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDQ7015045.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIANLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(6044..6742)
                     /gene="tapT"
                     /locus_tag="QBH27_002751"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6044..6742)
                     /gene="tapT"
                     /locus_tag="QBH27_002751"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDQ7015046.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSHFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6811..7230)
                     /gene="trxC"
                     /locus_tag="QBH27_002752"
     CDS             complement(6811..7230)
                     /gene="trxC"
                     /locus_tag="QBH27_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDQ7015047.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7437..8474
                     /locus_tag="QBH27_002753"
     CDS             7437..8474
                     /locus_tag="QBH27_002753"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDQ7015048.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8522..9211)
                     /gene="ung"
                     /locus_tag="QBH27_002754"
     CDS             complement(8522..9211)
                     /gene="ung"
                     /locus_tag="QBH27_002754"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDQ7015049.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9516..9899
                     /gene="grcA"
                     /locus_tag="QBH27_002755"
     CDS             9516..9899
                     /gene="grcA"
                     /locus_tag="QBH27_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDQ7015050.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9955..10542)
                     /gene="eamB"
                     /locus_tag="QBH27_002756"
     CDS             complement(9955..10542)
                     /gene="eamB"
                     /locus_tag="QBH27_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDQ7015051.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10645..11526
                     /locus_tag="QBH27_002757"
     CDS             10645..11526
                     /locus_tag="QBH27_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7015052.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11559..12893)
                     /gene="srmB"
                     /locus_tag="QBH27_002758"
     CDS             complement(11559..12893)
                     /gene="srmB"
                     /locus_tag="QBH27_002758"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDQ7015053.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13025..13762
                     /gene="trmN"
                     /locus_tag="QBH27_002759"
     CDS             13025..13762
                     /gene="trmN"
                     /locus_tag="QBH27_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDQ7015054.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13747..15369)
                     /gene="nadB"
                     /locus_tag="QBH27_002760"
     CDS             complement(13747..15369)
                     /gene="nadB"
                     /locus_tag="QBH27_002760"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDQ7015055.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            15625..15780
                     /gene="rseD"
                     /locus_tag="QBH27_002761"
     CDS             15625..15780
                     /gene="rseD"
                     /locus_tag="QBH27_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDQ7015056.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15777..16352
                     /gene="rpoE"
                     /locus_tag="QBH27_002762"
     CDS             15777..16352
                     /gene="rpoE"
                     /locus_tag="QBH27_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDQ7015057.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16385..17035
                     /gene="rseA"
                     /locus_tag="QBH27_002763"
     CDS             16385..17035
                     /gene="rseA"
                     /locus_tag="QBH27_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDQ7015058.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17035..17991
                     /gene="rseB"
                     /locus_tag="QBH27_002764"
     CDS             17035..17991
                     /gene="rseB"
                     /locus_tag="QBH27_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDQ7015059.1"
                     /translation="MKQLWFAMSLVTGSLLFSANALATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            17988..18467
                     /gene="rseC"
                     /locus_tag="QBH27_002765"
     CDS             17988..18467
                     /gene="rseC"
                     /locus_tag="QBH27_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDQ7015060.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18665..20464
                     /gene="lepA"
                     /locus_tag="QBH27_002766"
     CDS             18665..20464
                     /gene="lepA"
                     /locus_tag="QBH27_002766"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDQ7015061.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQIDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20480..21454
                     /gene="lepB"
                     /locus_tag="QBH27_002767"
     CDS             20480..21454
                     /gene="lepB"
                     /locus_tag="QBH27_002767"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDQ7015062.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
BASE COUNT         5246 a         5362 c         5631 g         5223 t
ORIGIN      
        1 ttcaacagcg aatacgtctt ccccaaattg cccacttcca tacttcctcc ttaccagaaa
       61 tctatcctta agctccttaa taaccatttt cctgctaact aaattcatgg ttaaggttgt
      121 ataatgatgc gcaacaaatg tataacattt tctttaccaa aaaaaataaa caaaagcgac
      181 cgacaaaagc atcggattac ggcaggagac ataatggcat ggctgaaagt actgtaacgg
      241 cagacagcaa actgacaagt agtgatactc gtcgccgcat ttgggcaatt gttggggcct
      301 cttcaggtaa tctggtcgag tggttcgatt tctatgtcta ctcgttctgt tcactctact
      361 tcgcccacat cttcttccct tccgggaaca cgacgactca actactgcaa acagcaggtg
      421 tttttgctgc aggatttctg atgcgcccaa taggcggttg gctgtttggc cgcatagccg
      481 ataaacatgg tcgcaaaaaa tcgatgctgt tatcggtgtg tatgatgtgt ttcggctcac
      541 tggttatcgc ctgtttgcca ggctacgaaa caatcggtac ctgggctccg gcattattgc
      601 ttctcgcgcg tttattccag ggattatccg ttggcggaga atatggcacc agcgccacct
      661 atatgagtga agttgccgtt gaagggcgca aaggttttta cgcatcattt cagtatgtga
      721 cgttgatcgg cgggcaactg ctagctctac tggttgtcgt ggttttacaa cacaccatgg
      781 aagacgctgt actcagagag tggggatggc gtattccttt cgcgttagga gctgtgttag
      841 ctgttgtggc gttgcggtta cgtcgtcagt tagatgaaac ttcacaacaa gaaacacgcg
      901 ctttaaaaga agctggatct ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg
      961 ttctcggttt taccgctgcg ggctcccttt gtttctatac cttcaccact tatatgcaga
     1021 agtatctggt aaatactgcg ggaatgcatg ccaacgtggc gagtggcatt atgactgccg
     1081 cattgtttgt attcatgctt attcaaccaa ttattggcgc gctgtcggat aagattggtc
     1141 gccgtacctc aatgttatgt ttcggttctc tggcaaccat ttttaccgtt cctattctct
     1201 cagcattgca gaacgtttcc tcgccttatg ccgcttttgg tctggtgatg tgcgccctgc
     1261 tgatagtgag tttttatact tcaatcagtg gaatactgaa ggctgagatg ttcccggcac
     1321 aggttcgcgc attaggcgtt ggtctgtcat atgcgttcgc caatgctata tttggtgggt
     1381 cggcggagta cgtagcgttg tcgctgaaat caataggaat ggaaacagcc ttcttctggt
     1441 atgtgacctt gatggccgtg gtggcgtttc tggtttcact aacattgcac cgtaaaggaa
     1501 aagggatgcg gctttagtga cgggtcagtt gccagacggt atagccggtg cttgcaccgg
     1561 cgatatccca ggcaaaatcc ttccagctcc agccgctccc ctcagggcgg ctatcccaaa
     1621 gctcttttga cgcccccaga ctgacagaga acatcaatcc aaacatggca ctgcgatccc
     1681 ggctcatccc ctgatgctgt gaatattcat ttccggcggc ggaaagcatc gccgaggcga
     1741 taaagtgttg agctttatcc tgcccgctcc agttatcgtt agccatatgg ctgcatccag
     1801 aaagtaacaa tagcgaacag acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac
     1861 cccgtacagg acggggttgt gattacagga tgcggctaat taatcggtcg atgcggatac
     1921 ggcgcaaacg gcggatgagt ttacgaacct tcaccggata atcggcaata ctttgcagat
     1981 cgcggtagtg cttaacgatg gtggtatgct cgcggatcag atccagttct ttctctcgct
     2041 gtggcgcaag ctccagttgc ggatcgtgga tcaaaatggc gttttccaga tccagacgcc
     2101 aggcgcgcgg gttcaggtta ttaccggtga tcaaaatcca cttatcatca acccacatcc
     2161 ctttcaggtg ataggtgttg tcgtcatctt tccataaccg aactactaac tggtcagtgt
     2221 tgacgtaata ctgtaaacga ctcaggaaac gacgcagatt gatctcatag agataaggca
     2281 atgcgccaat tatcttgaaa ggttcatctt ccggaatgta gaagtcattc gcggttttat
     2341 caccaacaat aatttcgacc tttttccctt cgcgcagcaa ctggataata ttgcgcacaa
     2401 ggattgctgg taggttgaag taaggtgtgc agatggttag tttctgctcg gcacaaggca
     2461 taagatggaa aatagtcttg ttcaacagac tcgatttccc cagccccact agcggcgtta
     2521 cggaaagctg atcgttgtcg gcatcgccct ggaaatgata agcggcatca cgcagctcct
     2581 ggcggaacag gcgaatgtcg ttcttgattt ccgggctttt tggccgatta acatcatcca
     2641 gacgattaac gccgcggcca ttcataatat tctgtgtaac ccattcaaac ataatgtctg
     2701 acatcttacg gttacggatc agatgataac ggtcgtagcg atatttatcg tgctgatgca
     2761 ggtaaacatc gttcaggctg gcaccgctat aaagtacgct atcgtcgatg ataaagcctt
     2821 taaagtgcag aacaccaagg gcttcacggg tattgattgg aacgccataa accggaacat
     2881 ttacgcccgg attttcctgc gccatgcggc agtaccagtc agcgttagtg ttagatgccg
     2941 cagcgccaat gcgtccacgt tgtgcacgat gccagtcgac cagcacccgc acatccagtt
     3001 ccggacgctg ccttttagcc tcatacaacg cgttcagaat gcctttgcca ccgtcatcct
     3061 gttcgagata cagggcaaca atgcaaatgc gctgcttcgc gctggctatt ttttccagca
     3121 acgtctcccg gaagtcggcg ggagcgtaaa agaaatcgac atcatcaact gattgagaaa
     3181 tcttgggtag ttgggcaagg tgttgttgat gtttattacg cttaaatttt gacaacatca
     3241 cagtgcattt cttctctgtt cattgaaggg tcctctgtgc aatgcagacg acataagcgg
     3301 gcaataataa caccagtccc gattaagtgg tcaacatttc cagtacctta ctcatgattc
     3361 ctcgcgctgg gcaagactta gcgtaagccc aacgatcccc tcttcgagct ggatatcaac
     3421 gttaaacccg agcttgcggg ccagcgccac catgccacga ttgtttggca tcgtaatacc
     3481 attcagacgt tgtagtccgt gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg
     3541 acctaagcct aaccctttaa gatccgagcg aaccagtacg gcaaattcgg catcgatgtt
     3601 atcagggtcg gagatcgcac gtgtgacgcc gaggatctct tccgtttgat caattcgtcg
     3661 taccgctaca aatgccattt cccgatcgta gtcgatctgc gtcatgttgg ctaaatcttc
     3721 atgggtaaat tcgttgatct cgctaaagta gcggtaataa agatcttctt tggtgactcg
     3781 cgaaatgaat tgctgaagct gtggctcatc ttctggcaaa atcgggcgga acaagcagcg
     3841 ttcaccgttt ttcaattcta cccattcttc cagctgatgc ggataagggc gcacagccag
     3901 tcggctctcg ttatcacctt caaacggcgc gatatccagc gtgacatcca gcgcggtaaa
     3961 ttcactgcca gaagccagca aaggatgaat atccagacgc tgaatttccg ggcaatcgac
     4021 aatcaagttg gaaacctgca ccagaagctg gctcaagcct gcaacatcca atgggcgtag
     4081 cgcactgcgc gcacgaatct ttttactttt gatcccctga ataaccagat agcgggccag
     4141 gttcatgttc agcggcggca gtgcgacgac ggcttgatct tcaggacgcc actccacacc
     4201 gccttcaccc agcatgatca acggcccgaa aaccggatcg tgctcaacca caacccgcaa
     4261 ctcctgagcg ccagcacggt tagccatact ttgcaccaac aggccgtgga cccgcgcctg
     4321 cggccaggcc atttttacgc gatcgaaaat agcgtttgcc gcttgctgga cttcattggc
     4381 tgtacgtagg tagagcatga cgccctgaac ttccgattta tgtggaatat ccggcgagcg
     4441 caatttcagt gccaccggat agccaatctg ttcggcaata tgtaccgctt cggtgctatc
     4501 actggcaatc caggtaggga gagtattcat accatacgct tgcaggatgg gctgaacttc
     4561 atgggtatcg agcgacgtag ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc
     4621 tgcggtattg gaagtcagat tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt
     4681 acgccggtac tcgaccatat gcataaaagc agtgatggtt ccttccggag tacggtaggt
     4741 cggcagcccg gcttcgctga ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca
     4801 gttcgtcagc aaagagacat atttgctgcg gggatgatgc tttaccgctt caattaatac
     4861 ttgcgcgctt tctgttgcgg gagcagcggc gctgggcgaa tgaataacca tcagcgcatc
     4921 aaaatcctgg ctgtggagca gaatgtccag cgttttgaca taatgctcgc tgctggcgtc
     4981 atcgcgcaga tcgagcgggt tagagattgc cacatgccct ggcagtgcat cgcgcagttt
     5041 ctggcaggtt tcttcgctta gcgttgccag cttgccattg cgtgaccata aggcatccag
     5101 cgccagcgcg gcaggcgcag caccgttgct gataatcatc aaccggtcgc cacgcagcgg
     5161 gcgcatatgg ctaagggttt ccaccgccga aaacagttcg tgggtgtcct gtacccgcaa
     5221 caaaccggca cgctgaatag ctgcatccca cgccggatcc attcctgccg tggtgttcaa
     5281 cagacgttga gccgccgggc tacgtccgct tttaatcacc aaaatcggtt tattgcgcga
     5341 ggcactacgg gctgccgaaa caaagcgtcg cgcgtcgctt aactgttcga gatagagcag
     5401 gatggcgctg gttttactgt cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc
     5461 caggctgtcg ccgagcgcaa taaagtagga aaagcccatc tcacgctgtt gcgcccagtc
     5521 gaggatggtg ttggagacag cagccgattg cgaaataaac gccagcttgc cgcgtttaat
     5581 cggcacaggc gaaaagctgg cattcagacc ttgccaggga gccagtaagc ccagactgtt
     5641 tggtcctagc aggcgcatgt tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga
     5701 tgccggggcg gaaagaataa tgcaggtttt acagcctttc tcgccgagct cttccagaag
     5761 agcaagatta cggctggcat tggtacataa aaccgcaagg tcgggtgtaa agggcaagtt
     5821 ggcaatatcc ggccaggcca acacacccaa caccgctttc caggctggcg tcaccgggag
     5881 taccggtccg ttaaagcctc ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt
     5941 gggtttcatc gatgcgccaa ttaccgcaat cgattttggt cgcagtagtg cttccagtcc
     6001 tcgctgactc ataccggtct cccgcagaag tgaccgaatg attttaaacg ctttctaact
     6061 gttctgctgt gatgcttccc ggatgttgcg tttttcctgc cagatagcgt gttttaaagc
     6121 gggtaaaatg ctcgcctaac cctgtcgccg ccctggtatc gccggccata tctaacagtg
     6181 cgatggctac ctcggcagta caatattggc cttcagcctg ggcttcacgc aggcgatagg
     6241 cagaaagccg agaaagatcg acggaaatga cgggaagatt atccagatac ggacttttac
     6301 gaaacatctt gcgagcttct ggccaggtac catcgagcat gataaatagc ggaggcttat
     6361 cggcaggtgg tgtgaagatc acttcccgtt gctcatcagc atacgaagcg ggaaagacca
     6421 ccattggctg atagtctggg ttttgcacca gatccagcaa atcttgcgag ggttcggtac
     6481 gcgaccattg aaacgcaacg gtatcaggca aaatatcagc aatcagacgc ccggtattac
     6541 tgggcttcat tggctcggtg tcgaacatca gcaaacagaa gtgacttttt gcttgtgctg
     6601 gggtaattgt cgaacagaga cataatttct ctggcaaaag acagcgttgg cagcgacgaa
     6661 cgcgattacc gcgggcaaga aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact
     6721 ggagaacagc gttttcggtc ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga
     6781 aaacggggca caagatgcgc cccggtaaga ttaaagagat tcgttcagcc agctgtcgaa
     6841 cggcgctttc ggtactgcgc cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat
     6901 gatcgtcggt atactacgaa ttccaaagcg actgctcaat tcacgttcag cttcggtatt
     6961 cactttcaca aagcgcactt taccgctacg ctcttgcgcg acatcttcaa aaattggtgc
     7021 gaaattacgg caggggccgc accacggtgc ccagaagtcg atcaccacag gtagatcatc
     7081 tttcagcaat ttgtcgagcg tttcaccggt cgcattaatc acctctccgt caaacaagtc
     7141 gtgaccgcag cgtccgcatt ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat
     7201 ggcctgacaa tgggtacaaa cggtattcat aactaacctc gggatgagta aggagactta
     7261 tcggcaacga cgccggtatg tttctaatat gttacatatt atcggggaca ttgttccaga
     7321 caacaatctg ttttattcaa tgggtatgat agacacaggc tttggaacga aatcatggcg
     7381 aaccgcgcgc acttcgggta atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga
     7441 acgatgaaat gaaaggtaaa agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg
     7501 attctgataa acgtacccac aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat
     7561 ctcgtgctga cggtggccgt cgccccgccc gcgatgacaa acagagtcag caccgtgacc
     7621 gcaagtggga agattcgccg tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg
     7681 aaaaggccga tcacggtggc atcagtggta aaagttttat tgatccggaa gttttgcgtc
     7741 gtcagcgtgc ggaagaaacc cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga
     7801 gccgtccgga agcgattgtt cgcgcctggt ttatccagag cgtaacgcca cgttttaaag
     7861 aagccttgcg ctggatggcg gcaaaccgca aagcgtacca tgtggtggat gaagcggaac
     7921 tgacaaaagc gtcaggcacg gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta
     7981 acggtacaac cgtgcagcag tgggtaagcc aggcaggcgc gcaggattgt gtactggccc
     8041 tggaaaacga atctaacccg cataacctgg gtggcatgat gcgtagctgt gcgcacttcg
     8101 gcgtgaaagg tgttgtggtg caggatgcag cactgctgga gtctggggcg gctatccgta
     8161 ccgcagaagg cggcgcagag cacgttcagc cgattactgg cgacaacatt gttaacgtgc
     8221 tggatgattt ccgtcaggcg ggttacaccg tagtgacaac ttccagcgag cagggtaaac
     8281 cgctgttcaa aaccagtctg ccagcgaaaa tggtactggt gctgggtcag gaatatgaag
     8341 ggttaccgga tgccgcacgc gatccgaacg atctgcgcgt gaagattgat ggtactggca
     8401 acgttgccgg gctgaatatc tctgtcgcaa ccggcgttct tcttggcgaa tggtggcgtc
     8461 agaataaagc ctgataaatc agccgggtgg caactctgcc atccggcatt ttcccgcaaa
     8521 tttactcact ctctgccggt aatactggca tccagtcaat cggcgtctcg ccacgttgtt
     8581 ccagccactg atttgccagc acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa
     8641 gcggcgacgg atgcggtgct ttcagtacat gatggcgttg cttatctata atcgcccctt
     8701 tcttttgcgc atgtgatccc cacaacaaaa acaccacgcc ttcgcgatgc tgatttatga
     8761 ggctgatgac tttatcagta aacgtctccc agccgaggct ggcgtgggaa tgcgcctgac
     8821 cagcgcgaac cgtcaagacg gtattgagca gcagaacacc ctgacgcgcc cagctttcaa
     8881 gataaccatg attagggcgg gtgaaaccgg gaatagtatt ttccagctct ttatacatat
     8941 tcagcagtga cggtggtgtc gcaatgccgg ggcgaacgga aaatgccaga ccatgcgcct
     9001 gtcctggtcc gtgataagga tcctgaccga gaatcaccac tttaacgtca cccaactccg
     9061 taaagcggaa cgcgttaaag acatcttttt gtggtggata gatagtgacg ccggactgcc
     9121 gctcgctggc gacggtctga agggtattaa gaaaataggg ttgctgcttc tcttcagcca
     9181 gcacatcatg ccaggttaat tcgttagcca tctcgctctc ctgcgaatct ttaatccgcc
     9241 tagcttaact gcttctttct acagaacaaa atcatgctca ttttatagac aaaaagacat
     9301 gcaaaaaaat gtaaattggt aaaatttcac atctgcataa gttgatgtaa aacaataaaa
     9361 tccatttgtg accacacttg atatgtggtt tttattgatt taaatcaaag aatcaagggt
     9421 gtttgaggag tatatataca ctcaagcaac aatggtttta ccaattggcc gcgacaggct
     9481 gaacaaatca aataattttg ccggggaggc atcacatgat tacaggtatc cagattacta
     9541 aagccgctaa cgacgatctg ctgaactctt tctggttgct ggacagcgaa aaaggcgaag
     9601 cgcgttgcat cgttgcgaaa gcaggttttg cagaagacga agtggttgca gtaagcaaac
     9661 tgggtgacat tgaataccgt gaagttccag tagaagtgaa accagaagtt cgcgttgaag
     9721 gtggtcaaca cctgaacgtt aacgttctgc gtcgtgaaac tctggaagat gcagttaagc
     9781 atccggaaaa atatccgcag ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact
     9841 ctctgactcc ggaacagcag cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag
     9901 gcttaagtag caagttacta agcggaagag ggcgacctct tccgcttttt ttcgttaata
     9961 gaaaatgcgt accgcgcaat agaccagcaa cagggcaagc acgatattta actggcgacc
    10021 atactggcga aacaatcgct gaaacagatg ccccgccagc gcccagcaca cattgccaaa
    10081 cgtcccaatc atcgccagca aaacgctgac gccaactacc cagcttagcg cctgtgtttg
    10141 cggcagaaca aacgtcgaca gtgccgtaac accgtacaaa atgattttga cgttcacaaa
    10201 ctgcaaagca aagctggccc aaaagctgat tggttttgcc tgaagtccgt cttcctttgt
    10261 tgggctggtg gcgattttcc acgccagcca gacaatatat gccgcccccg cccaactcaa
    10321 aaggtgtacc gctgccgggt caatcactgc cagtgaaaat gaaatgcccg cacacagtaa
    10381 catcacaatc aaaaatccca gactcatccc tgccagcaca cgggtacttt gacgaaatcc
    10441 atgcgacgta gcagagctaa gggcgagaat attgttcggt cctggcgtca tggcggtaat
    10501 cagggtgtaa gtccaaaaag cacttaaaag ggtcggtgtc acttgttatc tcctctgttt
    10561 tggtagacag tacccgacac ggaacattta aaaaaatgaa tgtttttaca ttctaaattc
    10621 gatattttcg ataggtttgg ggttatggat ctgcgccgtt ttattacgct taaaaccgtg
    10681 gtggaagagg gttccttttt gcgagcttcg caaaaattgt gctgtacaca atcgacggtg
    10741 acttttcata ttcagcagct tgagcaggag ttctcggtac aattgtttga gaaaattggt
    10801 cgacgaatgt gccttacccg cgaaggaaaa aagctgctgc cgcacatcta tgaacttacc
    10861 cgagtgatgg atacgcttcg tgaggctgct aaaaaagagt cggatccgga cggggaacta
    10921 cgcgttattt caggcgaaac gttgctctct tatcgaatgc cgcaggtgtt gcagcgtttt
    10981 cggcaacgcg ctccaaaagt gcgtttatcg ttgcagtcgc tgaactgtta cgtgatccgt
    11041 gatgcgctac tgaatgatga ggccgatgtc ggggtttttt atcgcgtagg gaatgatgat
    11101 gctctgaacc gacgagagtt gggtgaacaa ccgctggtgc tggtggcttc accgcaaatt
    11161 gcagatgtcg attttactga gccgggaaga cataacgcct gtagctttat tatcaacgaa
    11221 ccacaatgtg tcttccggca gatatttgag agcacgctgc gccagcggcg gatcacggtg
    11281 gaaaacacca ttgagcttat aagtatcgaa agcatcaagc gttgtgtcgc ggcaaatatc
    11341 ggagtcagtt acctgccgcg ttttgcggta gtaaaagagt tggagtgcgg agaattaatt
    11401 gaattgcctt ttggcgaaca gtcgcagacc attacggcaa tgtgcgctca ccatgccgga
    11461 aaagcggtta gcccggcgat gcacactttt attcagtgtg ttgaagagtg ttttttgccg
    11521 ggataaaaac aatgccgggc ttaaacccgg cattgagatt actcttctgt cgtttgcggt
    11581 ggcacgcccg ttccgcttgg tttacggcgc ttaccaatat ttttagtgtc gcgatggcgt
    11641 tttttcaccc gcggcttctc tttctcttta gcttttttct tctcggcacg tttagccaac
    11701 actttcttcg atggcttgcc ggtctgcttc tcgcttggcg cacgcgtttt cggacgtaac
    11761 tcatcaatta cgcgagcttt aattggctct tcaatataac ggccaacttt gcccagcagc
    11821 agatggtcat gggcttccac cagcgaaatc gcggtgcctt tacgaccggc gcgcgcggta
    11881 cgtccgatac ggtgcaaata agtatcgcca ctgcgcggca tatcgaagtt aaagacgtgg
    11941 ctgacgtcag gaatgtcgat accgcgcgcg gcaacatcgg tagcaaccag tacgtttacg
    12001 cggccttcgg tcaagcgttt aatcgcttcg ttacgcttgc cttgtaccat ctcaccttcg
    12061 agatagcagt tgttgatgcc tgcttcgcgc agccagtttg ccagctcatg cacacgctca
    12121 cgcttacgca caaacacaat tgagcgggtc gcttccggct gttttaacag atgcaccagc
    12181 aacgcggttt tatgctcaag atcatcggcg cggtaatacc actgatgaat ttttttgcgc
    12241 tcacgggtgg acggattggc agaaacttcc accggatctt ccaacagacg ctcggcaaag
    12301 tcctgaatcg catcgccttc cagcgttgcc gaaaagagca gggtctgttt acgccagcgc
    12361 gtttcgccag caatatgttc gatatcctgg gcgaagccca tatccagcat acggtctgct
    12421 tcgtcgagga tcagcgtttc aaccgcgcgg caatcgaagt tctcttcttt tatgtattgc
    12481 agcagacgtc cggtcgtggc gaccacaatg tcctggtttt cgctgaacac ttctgcgtgg
    12541 ttcatatagg ccacgccgcc ggtgatggtg gcaatatcca gatgcgtatg tttcgccagt
    12601 tcgcgggcat gatcggccac ctgcatcgcc agctcgcgag ttggggtgag gatcaaaata
    12661 cgcggcggac cggatttctt acgtgggaaa tcgagcaggt gctgcaacgc tggcagcaga
    12721 tacgccgccg ttttaccggt gcctgtcggc gcagaaccga gtacatcacg gccatcgagc
    12781 gcaggcggaa tggcggcagc ctgaatggcg gtcgggcgag tgaaaccttt atcctggagg
    12841 gcttccagca ggctttcgtc gagttcaagt tcggaaaaag tcgttacagt catgttctac
    12901 ctctgtgtgg ggcgctgatt atagacgtta cggctgcaat cttcatctgt ttgtatggat
    12961 atcgcttctc gggtattgtt ctcacccggt gctatcgccg tttttcctgc aaggttttat
    13021 tttcatgtca cagtctacat ccgtgtttcg tcgtaatgga tttactttta aacagttttt
    13081 tgttgctcac gatcgctgtg cgatgaaagt gggaacggat ggcattttgc tgggcgcatg
    13141 ggcaccggta gctggggtaa aacgttgcct tgatatcggc gcgggtagcg ggttgctggc
    13201 attaatgctg gcgcagcgaa cagatgacag cgtgatgatt gatgcagttg aactggaaag
    13261 tgaagctgcc gcgcaagcgc aggaaaatat caaccagtcc ccgtgggcag agcggattaa
    13321 tgtccatacg gcggatattc tgcagtggat cacacagcag acagtacgct tcgatttaat
    13381 catcagcaac ccaccttact atcagcaggg agtagagtgc gcgacacctc aacgggaaca
    13441 ggctcgctat accactacgc ttgatcaccc atcgttgctg acttgtgcgg cggagtgcat
    13501 taccgaagag ggatttttct gcgtggtgct gccagagcag attggtaatg gttttactga
    13561 actggcatta agtatgggct ggcatttacg tttgcggacg gatgtggcgg aaaacgaagc
    13621 gcgactgccg catcgggtgc tgctggcatt ctccccgcag gcgggagagt gctttagcga
    13681 tcgcttagtc attcgtgggc cagaccagaa ctattccgaa gcgtatacgg cgctgaccca
    13741 ggctttttat ctgtttatgt aatgattgcc ggcagaaagg atcgatggac cggaatgcgt
    13801 gagcagttcc ggataatcca gcgtgaaatg caggccacgg ctctccttgc gcatcatcgc
    13861 acagcgaaca atcaactcgg caacctgtac cagattacgc agctccagca aattatttga
    13921 gacgcggaaa tgggcgtaat attcgtctat ttcttgttgg agcatggtta tccgccgcag
    13981 ggcgcgttcc aggcgcttcg ttgtgcgaac aatgccaacg taatcccaca taaacagacg
    14041 tagctcgtgc cagttatgct gaattactac ccgttcgtca gggttctcaa cgcggctttc
    14101 atcccacggc ggtaacgtac tgacgccgtg ggcataaggt atacgtctgc tgatatcttc
    14161 tgccgccgac cagccataga ccagacactc cagcaatgaa ttcgaggcca tgcggttagc
    14221 gccgtgtaag ccggtataac tcacttcgcc aatggcatac aaaccctcga cgtccgtacg
    14281 cccatgatca tcaaccatta caccaccgca ggtataatgt gcagcaggca caatcggcac
    14341 gggttctttc gtgagatcta tccccagccc gagcaatttt tcataaatca tcgggaaatg
    14401 ctggcgaata aaatcggcgg gcttatggct gatgtcgagg aacatacaat ctgcgccgag
    14461 gcgtttcatt tcatggtcaa tggcgcgggc gacaatatcg cgcggggcca gttcgccgcg
    14521 ctcatcaaaa tcgggcataa aacgcgtgcc atccgggcgc ttgagataag cgccttcgcc
    14581 gcgcagtgct tccgttaaca ggaaattgcg tgcctgtggg tgatacagcg cggtagggtg
    14641 gaactgatta aattcgagat tggcaacccg gcagcctgcg cgccacgcca tagcaatgcc
    14701 atcgccagaa gaaatatccg gattggtggt gtactgataa acttttgaag caccgccggt
    14761 tgccagtacc actgcttttg cgtggcaggt ttcaaccgtt tctttatttc ggttccatac
    14821 ccacgcgcca acaacccgtc gcgtgcccgg caggccaatt ttgtcagaaa caatcagatc
    14881 aaccgcgttg ctgcgctcca gcacgcgaat attcggatgg ttctgcgcct tgcccaccag
    14941 cgtggtttct acttctctac cggtggcgtc ggcggcatga agaatacgac ggtgactatg
    15001 tccaccttca cgagtcagat ggtagctttc ttcgccattc ggctgaatgt gggtatcaaa
    15061 caacaccccc tggtcgatta gccattgcac acaggatcgt gcattactgg cgacaaattc
    15121 aactgcatgg cgatcgcaaa taccagcccc ggcaatcaat gtgtcttcca catgcgagtc
    15181 aatgctgtca gtttcatcaa acacggcggc aataccgccc tgggcataaa atgttgaacc
    15241 ttccgttacc gggcctttac ttagaacgat gacctgatgc tggtcagcca ggcgtagcgc
    15301 cagtgaaagt ccagctgcgc cgctaccgat aatcaacacg tcacatgaat gttcagggag
    15361 agtattcatt ttctttgttt aatttactaa acactgtttg gtcagcataa catcatgttg
    15421 tgcaaataaa cacctgctat tttaatattt gttacagtga ctaaacacgc tggctcaggg
    15481 cggcgagaaa aagagaagtt actggctggt ggaggattag gtggtgaaat aaaaaggccg
    15541 ttgggttact cttcaggcag ttaaatgggc atttctacac agataatgcg atgttcagat
    15601 tctgtagact tataatgata gataatgatc cgtctacagc atgacaaaca aaaacagatg
    15661 cgttacggaa ctttacaaaa acgagacact ctaacccttt gcttgctcaa attgcagcta
    15721 atggagtggc gtttcgatag cgcgtggaaa tttggtttgg ggagacttta cctcggatga
    15781 gcgagcagtt aacggaccag gtcctggttg aacgggtcca gaagggagat cagaaagcct
    15841 ttaacttact ggtagtgcgc tatcagcata aagtggcgag tctggtttcc cgctatgtgc
    15901 cgtcgggtga tgttcccgat gtggtacaag aagcttttat taaagcctat cgtgcgctgg
    15961 attcgttccg gggagatagc gctttttata catggctgta tcggattgct gtaaatacag
    16021 cgaaaaatta cctggttgct caggggcgtc gtccaccttc cagtgatgtg gatgccattg
    16081 aagctgaaaa cttcgaaagt ggcggcgcgt tgaaagaaat ttcgaaccct gagaacttaa
    16141 tgttgtcaga agaactgaga cagatagttt tccgaactat tgagtccctc ccggaagatt
    16201 tacgcatggc aataaccttg cgggagctgg atggcctgag ctatgaagag atagccgcta
    16261 tcatggattg tccggtgggt acggtgcgtt cacgtatctt ccgagcgagg gaagctattg
    16321 ataacaaagt tcaaccgctt atcaggcgtt gacgatagcg ggatactgga taagggtatt
    16381 aggcatgcag aaagaacaac tttccgcttt aatggatggc gaaacgctgg atagtgagct
    16441 gcttaacgaa ctggctcata acccagaaat gcagaaaacc tgggaaagct atcacttaat
    16501 ccgtgactca atgcggggtg atactcccga ggtgctccat ttcgatatct cttcacgcgt
    16561 gatggccgcc attgaagaag agccagtacg tcaaccggcg acattgatcc cggaagccca
    16621 gcctgcgccg catcaatggc agaaaatgcc attctggcag aaagtacgtc cgtgggcggc
    16681 acagcttacc caaatgggcg tagcagcatg cgtatcgctt gcagttatcg ttggcgtcca
    16741 gcactataat gggcaatctg aaacgtccca gcagcccgaa acgccagtat ttaatacact
    16801 gccgatgatg ggtaaagcca gcccggttag tctgggagta ccttctgaag cgaccgcaaa
    16861 taatggtcaa cagcagcagg tacaggagca gcgtcgtcgc attaatgcaa tgttgcagga
    16921 ttacgaactg caacgccgac tccactctga acagcttcag tttgagcagg cacaaactca
    16981 gcaagccgct gtacaggtgc caggaattca aactttagga acgcaatcgc agtaatgaag
    17041 caactttggt ttgccatgtc attagtgaca ggtagcctgt tattctctgc taacgccttg
    17101 gccactcccg cgtccggggc gttattacag cagatgaacc tggccagtca gtcactgaat
    17161 tacgagctgt catttatcag catcaataaa cagggtgttg agtctctgcg ttatcgacat
    17221 gcacgcctcg ataaccgtcc tcttgcacaa ttgttgcaaa tggatggccc gcgccgggaa
    17281 gtggtacagc gcggcaatga aatcagctat tttgaaccgg gacttgaacc gttcacgctt
    17341 aatggcgatt acattgttga ttctctgcca tcgctcatct ataccgattt caaacgcctt
    17401 tctccttact acgactttat ctctgtcggg cgcacgcgta ttgctgatcg tctttgcgaa
    17461 gtcattcgtg tggttgcccg agatggtaca cgctacagct acatcgtgtg gatggacacc
    17521 gaatcgaaat taccgatgcg ggttgatctc cttgatcgcg atggtgaaac gctggaacaa
    17581 tttcgcgtga ttgcttttaa cgtcaatcag gatatcggca gcagtatgca gacgctggcg
    17641 aaggcaaatt tgccgccgtt gctttctgtt cctgttggtg aaaaagctaa attcagctgg
    17701 acgccaacct ggctgccaca gggttttagc gaagtttcca gtagtcgacg tccgctaccg
    17761 acgatggaca acatgcctat cgaatcacgt ctctattccg acggattatt cagcttctcg
    17821 gtaaacgtta accgcgctac gccatcgagc accgatcaga tgttgcgcac cggacgcaga
    17881 accgtcagta caagcgtacg tgataacgcc gaaatcacca ttgttggtga actgccgccg
    17941 caaacggcga aacgcattgc cgagaatatt aagttcggga cagcgcaatg atcaaagagt
    18001 gggctaccgt cgtctcctgg gaaaacgggc aggcgctggt cagttgtgat gttaaagcct
    18061 catgcagcag ctgcgcttca cgcgccggtt gcggtagccg tgtgttgaat aaacttggcc
    18121 cgcaaaccac gcataccatt gtcgtaccct gtgatgagcc gctagtgccg gggcaaaaag
    18181 tggaattggg gatcgccgaa ggcagccttc ttagctccgc attactggtt tatatgtcgc
    18241 cgctggtggg attattcctc atcgcttcgc tatttcagct actctttgct tctgacgtcg
    18301 cagcattatg tggtgcgatc ctcggcggta ttggtgggtt cctgattgcg cgcggctatt
    18361 cgcgtaagtt tgctgcccgg gcagaatggc aaccgatcat cttaagcgtg gcattgccgc
    18421 ctggccttgt gcgatttgaa acatcttcgg aagacgcgag ccagtgattt cactggcttt
    18481 gttgcatttt gcataacaaa tgcgaggacg tttccatata ccacaatcca tacggtatga
    18541 acatttcctc gcctcaatgt tgtagtgtag aatgcggcgt ttctattaat acagacgtta
    18601 agctcagaac agcgacttct aaaagcctgg ttaaccgggc attaaggcac aataatcata
    18661 ctttatgaag aatatacgta acttttcgat catagctcac attgaccacg gtaaatcgac
    18721 gctgtctgac cgtattatcc agatctgcgg tggcctgtct gaccgtgaaa tggaggcgca
    18781 ggttctcgat tccatggatc ttgagcgtga gcgtggcatt accatcaaag cgcaaagcgt
    18841 gacgctggac tacaaagcgt ctgacggcga aacctatcag cttaacttta tcgacacccc
    18901 gggccacgta gacttctcct atgaagtttc tcgttcgctg gctgcctgtg aaggtgcatt
    18961 gctggtggtc gacgccgggc agggcgtaga agcgcaaacc ctggcaaact gctacaccgc
    19021 catggaaatg gatctcgaag tggtgccggt actgaacaag attgacctgc cggcagccga
    19081 tcctgaacgc gtggcggaag aaattgaaga tatcgtcggc atcgacgcca ccgacgcggt
    19141 gcgctgttca gcgaaaaccg gcgttggcgt gcaagacgtt ctcgaacgct tggtgcgcga
    19201 cattccgccg ccggaaggcg atccggaagg cccattgcag gcgctgatta tcgactcctg
    19261 gttcgacaac tacctgggcg ttgtttcact tatccgtatt aaaaacggca ccctgcgtaa
    19321 gggcgacaaa gtgaaagtca tgagcaccgg gcagacctat aacgccgatc gtctgggcat
    19381 cttcacgccg aagcagattg accgcactga actgaaatgt ggcgaagtag gctggctggt
    19441 atgtgcgatt aaagatatcc acggcgctcc agtaggcgat accttaacac tggcgcgtaa
    19501 tccggcagaa aaggcgctgc ctggctttaa gaaagtcaaa cctcaggtat atgctggtct
    19561 gttcccggta agttccgacg actatgaagc cttccgtgac gcgctgggta aactcagcct
    19621 gaacgatgcc tcactgttct atgagccgga aagctccagc gcgttgggct ttggtttccg
    19681 ctgcggcttc cttggcctgc tgcacatgga gatcatccag gaacgtctgg aacgtgaata
    19741 cgatctggat ctgatcacca ctgcgccgac tgtagtgtat gaagttgaaa ccacgtcaag
    19801 agaagttatc tacgtcgaca gcccatccaa gctgccagcg gtaaataaca tttacgaact
    19861 gcgcgagccg attgcagagt gtcacatgct gctgccgcag gcatatctcg gcaacgttat
    19921 tacgctgtgc gtagaaaaac gcggcgtgca gaccaatatg gtttaccacg gtaatcaggt
    19981 ggcgctgacg tacgagatcc cgatggcgga agtggtgctc gacttcttcg atcgcctgaa
    20041 atctacctcg cgtggttatg cgtctctgga ttacaacttc aaacgcttcc aggcgtccga
    20101 catggtacgt gtagacgtat taatcaacgg tgaacgtgtt gatgcattgg cgctgatcac
    20161 ccaccgtggt aattcgcaaa accgcggtcg cgagctggtg gagaagatga aagatctgat
    20221 cccacgccag cagtttgata tcgccattca ggcagcgatt ggtacgcaca tcattgcgcg
    20281 ctccaccgtg aaacagctgc gtaaaaacgt actggctaaa tgttatggcg gcgatatcag
    20341 ccgtaagaaa aagctgctgc agaagcagaa agaaggtaag aaacgcatga agcagatcgg
    20401 taacgtcgag ctgccgcagg aagcgttcct cgccattctg cacgtcggca aagacaacaa
    20461 ataaccctta ggagttggca tggcgaatat gtttgccctg attctggtga ttgccacact
    20521 ggtgacgggc attttatggt gcgtggataa attctttttc gcacctaaac ggcgggaacg
    20581 tcaggcagcg gcgcaggcgg ctgccggtga ctcactggat aaagcaacgc tgaaaaaggt
    20641 tgcgccgaag cctggctggc tggaaaccgg tgcttctgtt tttccggtac tggctatcgt
    20701 attgattgtg cgttcgttta tttatgaacc gttccagatc ccgtcaggtt cgatgatgcc
    20761 gactctgtta atcggtgatt ttattctggt agagaagttt gcttatggca ttaaagatcc
    20821 tatctaccag aaaacgctga tcgaaaccgg tcatccgaaa cgcggcgata tcgtggtctt
    20881 taaatacccg gaagatccaa agcttgatta catcaagcgc gcggtgggtt taccgggcga
    20941 taaagtcact tatgatccgg tctcaaaaga gctgacgatt caaccgggat gcagttccgg
    21001 ccaggcgtgt gaaaacgcgc taccggtcac ttactccaac gtggaaccga gcgatttcgt
    21061 tcagaccttc tcacgccgta atggtgggga agcgaccagc ggattctttg aagtgccgaa
    21121 aaacgaaacc aaagaaaatg gaattcgtct ttccgagcgt aaagagacac tgggtgatgt
    21181 gacgcaccgt attctgacag tgccgattgc gcaggaccag gtggggatgt attaccagca
    21241 gccagggcaa caactggcaa cctggattgt tccgccggga caatacttca tgatgggcga
    21301 caaccgcgac aacagcgcgg acagtcgtta ctggggcttt gtgcctgaag cgaatctggt
    21361 cggtcgggca acggctatct ggatgagctt cgataagcaa gaaggcgaat ggccgacagg
    21421 tgtgcgctta agtcgcattg gcggtatcca ttaatagcca tc
//
LOCUS       DAOFSV010000071        21199 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.071, whole genome shotgun sequence.
ACCESSION   DAOFSV010000071 DAOFSV010000000
VERSION     DAOFSV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.071"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            139..1491
                     /gene="celF"
                     /locus_tag="QBH27_002768"
                     /gene_synonym="chbF"
     CDS             139..1491
                     /gene="celF"
                     /locus_tag="QBH27_002768"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDQ7015063.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1504..2262
                     /gene="chbG"
                     /locus_tag="QBH27_002769"
     CDS             1504..2262
                     /gene="chbG"
                     /locus_tag="QBH27_002769"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDQ7015064.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(2309..4570)
                     /gene="katE"
                     /locus_tag="QBH27_002770"
     CDS             complement(2309..4570)
                     /gene="katE"
                     /locus_tag="QBH27_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDQ7015065.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAVPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEHRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPNVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADRADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            4753..5016
                     /gene="cedA"
                     /locus_tag="QBH27_002771"
     CDS             4753..5016
                     /gene="cedA"
                     /locus_tag="QBH27_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDQ7015066.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(5063..6454)
                     /gene="tcyP"
                     /locus_tag="QBH27_002772"
     CDS             complement(5063..6454)
                     /gene="tcyP"
                     /locus_tag="QBH27_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDQ7015067.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6587..7177)
                     /locus_tag="QBH27_002773"
     CDS             complement(6587..7177)
                     /locus_tag="QBH27_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ7015068.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(7341..8009)
                     /gene="hxpB"
                     /locus_tag="QBH27_002774"
     CDS             complement(7341..8009)
                     /gene="hxpB"
                     /locus_tag="QBH27_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDQ7015069.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            8156..8692
                     /locus_tag="QBH27_002775"
     CDS             8156..8692
                     /locus_tag="QBH27_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDQ7015070.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(8733..9593)
                     /locus_tag="QBH27_002776"
     CDS             complement(8733..9593)
                     /locus_tag="QBH27_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDQ7015071.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(9699..9989)
                     /gene="ghoS"
                     /locus_tag="QBH27_002777"
     CDS             complement(9699..9989)
                     /gene="ghoS"
                     /locus_tag="QBH27_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDQ7015072.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            complement(10090..11019)
                     /gene="pfkB"
                     /locus_tag="QBH27_002778"
     CDS             complement(10090..11019)
                     /gene="pfkB"
                     /locus_tag="QBH27_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDQ7015073.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNHKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            11305..12063
                     /locus_tag="QBH27_002779"
     CDS             11305..12063
                     /locus_tag="QBH27_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDQ7015074.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(12116..12223)
                     /locus_tag="QBH27_002780"
     CDS             complement(12116..12223)
                     /locus_tag="QBH27_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015075.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(12394..13418)
                     /locus_tag="QBH27_002781"
                     /pseudo
     CDS             complement(12394..13418)
                     /locus_tag="QBH27_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            13942..15870
                     /gene="thrS"
                     /locus_tag="QBH27_002782"
     CDS             13942..15870
                     /gene="thrS"
                     /locus_tag="QBH27_002782"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDQ7015076.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            15874..16416
                     /gene="infC"
                     /locus_tag="QBH27_002783"
     CDS             15874..16416
                     /gene="infC"
                     /locus_tag="QBH27_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDQ7015077.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            16513..16710
                     /gene="rpmI"
                     /locus_tag="QBH27_002784"
     CDS             16513..16710
                     /gene="rpmI"
                     /locus_tag="QBH27_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDQ7015078.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            16763..17119
                     /gene="rplT"
                     /locus_tag="QBH27_002785"
     CDS             16763..17119
                     /gene="rplT"
                     /locus_tag="QBH27_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDQ7015079.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    17236..17360
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            17242..17286
                     /gene="pheM"
                     /locus_tag="QBH27_002786"
     CDS             17242..17286
                     /gene="pheM"
                     /locus_tag="QBH27_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDQ7015080.1"
                     /translation="MNAAIFRFFFYFST"
     gene            17570..18553
                     /gene="pheS"
                     /locus_tag="QBH27_002787"
     CDS             17570..18553
                     /gene="pheS"
                     /locus_tag="QBH27_002787"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDQ7015081.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            18568..20955
                     /gene="pheT"
                     /locus_tag="QBH27_002788"
     CDS             18568..20955
                     /gene="pheT"
                     /locus_tag="QBH27_002788"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDQ7015082.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGIHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            20960..>21199
                     /gene="ihfA"
                     /locus_tag="QBH27_002789"
     CDS             20960..>21199
                     /gene="ihfA"
                     /locus_tag="QBH27_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDQ7015083.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVT"
BASE COUNT         5452 a         4966 c         5441 g         5340 t
ORIGIN      
        1 agctatcgta agaaattgac tgaatttaat cagtaagtcg ttatacctga caattcacat
       61 atctgtcctg ttgctggatt atttatgtcc gggggcagat atgccagata tcagtattct
      121 gtactgaagg gagaaattat gagccagaaa ttaaaagtcg tcactattgg tggcgggagc
      181 agctataccc cggagttact ggaaggattt attaagcgtt atcacgaatt gccggtcagc
      241 gaattatggc tggtggatgt cgaaggtggt aaagcgaaac tggatattat ttttgatctc
      301 tgccaacgga tgattgataa cgctggcgtc ccgatgaagc tttataaaac gctggatcgc
      361 cgcgaagcat tgaaagatgc tgatttcgtt actacccaac tgcgcgttgg ccaattaccg
      421 gcgcgggaac tggatgaacg tattccatta agtcatggtt atcttggtca ggaaactaac
      481 ggcgcgggcg gtctgtttaa aggtctgcgt accattccgg tgatttttga catcgtaaaa
      541 gatgtcgaag aactatgtcc gaatgcatgg gtgattaact tcactaaccc ggcgggaatg
      601 gtcactgaag ccgtttatcg tcataccgga tttaaacgct ttatcggcgt gtgtaatatt
      661 ccgatcggca tgaagatgtt tattcgcgat gttctgatgc tgaaagacag cgatgattta
      721 tctatcgatc tgttcggcct caaccatatg gtgttcatta aggatgtgct ggtaaatggc
      781 aaatcacgct ttgccgaatt gcttgatggt gtggcatccg ggcagttaaa agcatctggc
      841 gttaaaaata ttttcgatct gccatttagc gaaggcttaa ttcgttctct gaatctgttg
      901 ccgtgttctt atttgcttta ttacttcaag caaaaagaga tgctggctat tgaaatgggc
      961 gaatactaca aaggcggcgc acgagcgcag gtcgtacaga aagtcgagaa acaacttttt
     1021 gagctatata aaaacccgga gttgaaagtt aagccgaaag aactggaaca gcgcggtggg
     1081 gcttattatt ctgatgctgc gtgcgaagtg atcaacgcta tctataacga caagcaagca
     1141 gaacattacg ttaatatccc gcatcatggg catattgata atattccggc agactgggcg
     1201 gtagaaatga cctgtacgct ggggcgcgat ggcgcgacgc cacatccgcg cattacgcat
     1261 ttcgatgata aagtgatggg gctgattcac accatcaaag gcttcgagat tgctgccagc
     1321 aacgccgcac ttagtggaga atttaacgat gtcttactgg cgctaaacct tagtccgttg
     1381 gtgcattccg atcgcgatgc tgagctgctg gcacgcgaga tgattctggc gcacgagaaa
     1441 tggctgccaa attttgccga ctgcatcgca gagcttaaaa aagcacatta accgaggctg
     1501 attatggaac gcttactgat tgttaatgct gatgattttg gcttaagcaa aggtcagaac
     1561 tacggcatta tcgaggcctg tcgcaatggg attgtcacgt cgacgacggc gctggtgaat
     1621 gggcaggcta ttgaccatgc ggtgcagttg agccgtgatg aaccgagtct ggccataggg
     1681 atgcactttg tccttactat gggcaagcct ctgacagcta tgccggggtt aacccgcgat
     1741 ggtgtgctgg gaaaatggat ctggcagttg gcagaagaag atgctttacc gctggaagaa
     1801 attactcagg agctcgccag tcagtatttg cgtttcattg agctatttgg acgcaaacct
     1861 acgcatcttg atagccatca tcatgtgcat atgttcccgc agattttccc gattgtggca
     1921 aagtttgcgg ctgaagaggg gattgcgttg cgcatcgacc gtcagtcgct ttctaaggat
     1981 ggtgatttac cggctaatct gcgcagttcg cagggattca gtagcgcgtt ttatggtgaa
     2041 gagattagcg agacactgtt cctgcaagtg cttgatgact ccagtcatcg cggcgagcgc
     2101 tcgctggaag tgatgtgcca cccggcgttt atcgataata cgattcgcca gagtgcttat
     2161 tgcttccctc ggttaacgga actggatgtg ctgacttcag cgtcgttgaa atatgcgatt
     2221 gctgagcgtg gttatcggtt gggtagttat catgatgtgt aagtaaggtg gtaaggtcac
     2281 tccccagggg gcgtgacctt atccatattt acgccgggat tttgtcaatc ttaggaatgc
     2341 gtgaccacac gcggtgtgct gtcatcagcg ttaacagttc atccataaaa ctaccatcgg
     2401 cgcggtcagc ttccgcaatc ccttcttcac cctggtcagc gaccttgatt gttgctttaa
     2461 acttgcgcgc gtctcctgcc agcgcaatcg gtttaaggtg tttgtaggct tccatcagat
     2521 agtagttggc atcgccgttg ttagcgatat ccgcaatatt gccgcaaggg acaatgactg
     2581 catcgaccgt cagcgaaggc gcaccggcaa aggtagcagc tataggcaac accgtaccgt
     2641 catccgcagt cacttccccc attcgcgagt agagcagttt ggcatgaacg cctttggcct
     2701 tcagcgcctt gagaatggcc agaaggtctg ccgatctcac ttcatcatta agcaaaattg
     2761 ctaccacgcg acctttcaca tcaccgtcag gaatggcgta cagacttaag gatggatcct
     2821 ttttcagacc gttgacgttc ggaggcgggg tgatattcaa ctggtcgtca gtcagttcga
     2881 taccgagatt tttcgccacc gcctgggcca gagtgagatc aatatgcgcc agctggtcaa
     2941 caacgcgctc acgaatatac ggccgaacta ctttgcttaa ctcaaaactg aaaccatcga
     3001 caatatggcg atgctcgaat ggcgtctgac ttagccagaa cagacgcgga tgggaataat
     3061 attcgccaaa cgatggactg cgctcgcgaa ctttattgcc ttccacgcgt tcctggtatg
     3121 attcaaaacc gccacgtttt ggccccggcg gtgtttcgcg cggccagtta tcgttgatcg
     3181 agttcggttc gtaattcgcc gggttagtgt cgatccccat acgatgcatg ccgtcacgct
     3241 ggaaattatg gtaagggcag gtcgggcggt taatcggaat ttcatggaaa ttcggcccac
     3301 caagacgact gatttgtgta tcggtataag agaacaaacg cccctgcaat aacgggtcgt
     3361 tggtgaaatc cagaccgggg acaatatgcc ctggatggaa agccacctgt tcgttttcag
     3421 caaagaagtt atccggatta cgattgagca ccattttgcc gacacgctga acgggcacca
     3481 attcttccgg gataagtttg gttggatcga gaagatcgaa gtcgaacttg aattcgtctt
     3541 cttcaggaat caactggaag cccagttcgt attcaggaaa atcgcctgct tcaatcgctt
     3601 cccacaactc gcggcggtgg aagtccgggt cacgtccggt tagtttttgt gcttcatccc
     3661 aaacgagtga ggctttacct gccagtggtt tccagtggaa acgtacgaac gttgccttcc
     3721 cttcggcatt aatcaggcgg aaggtatgaa taccgaagcc ttccatagtg cggtaactgc
     3781 gcgggatacc gcgatccgac attgcccaca tcacgttgtg cagagtttca ggttgcagag
     3841 aaacataatc ccagaaagta tcgtgggcac tttgcccttg tggaactgcc cagtgcggtt
     3901 ctggttttac cgcatgaaca aaatcgggga atttatgcgc atcctggata aagaagattg
     3961 gcgtgttatt gccaacgagg tcaaaaatac cctcttcagt atagaacttg gtggcaaagc
     4021 cacggatatc acgcacggta tcggcagagc cagcgccgcc ctgaacggta gagaacctaa
     4081 caaatactgg ggtgattttg ttcgtatctg agaggaaatc cgctttggtg atatcgctta
     4141 agcttttata tggctggaaa taaccgtgag ctgctgatcc gcgtgcatga acaatacgtt
     4201 ccgggatgcg ctcatggtca aagtgagtga ttttctcgcg cagaataaaa tcttccagca
     4261 gagttggacc acggctaccg gcacgcagtg agttttgatc gtcggcgatg cgcacgccct
     4321 gattagtggt cagcgcataa ttttcactgc ctttgcgtac gtcttccaga gaattaagtt
     4381 tttcgttacg cgtatcaggg gctttcaggc tccctggggc ggtaggttgt gcaccaggcg
     4441 gtgttggttc agccgctgga cgatgagatc cgtcttcagg tgccagtgag tccatccccg
     4501 gtttcgcttc gctggaatcg tgtagtggtg actggtgctg atgtgggttc ttttcgttat
     4561 gttgcgacat tgaactcgtc tccttaaatt attactgaaa gggccgctgt tttggtcaaa
     4621 aaaccctcta actatagacc accagccaga tcccgcttcg gaaactattc tggaaacggt
     4681 gttatgaaat acacgcaaag cgggacgtac aggggctaaa tcggctaaac tacagttttt
     4741 ctgattttta atatgcgttt agtgaagcca gtaatgaaga aaccgctccg tcagcaaaac
     4801 cgccagatta ttagctatgt cccacgcacg gaacccgcgc cgccagaaca tgcgataaag
     4861 atggattcgt ttcgagatgt ctggatgctg cgtggcaaat atgttgcgtt tgtactgatg
     4921 ggggagtcat ttctgcgctc accggcgttt actgtgcctg aatccgccca gcgttgggca
     4981 aatcagatcc gccaggaaaa tgaggtcgaa gaataaaaaa acacctgtcg ggatgacagg
     5041 cgtttaacgt aacaaatcag gattaacggt gtgccagttc ggcgtcgtct tcgctatcca
     5101 gaatggcttt atcggtttgc ttcagccact ggctggtcag cgtgccagcg gtcatcgagc
     5161 cactaacgtt tagcgccgta cggcccatgt cgataagcgg ttcaacggag attaacaatg
     5221 ccaccagtgt taccggcagg cccatcgcag gcagcacaat cagtgcggcg aaagttgcac
     5281 caccaccgac accggcaacg cctgcggaac taacggtgac aataccgacc agcgtcgcaa
     5341 tccacatcgg gtccagcggg ttaatgccca ccgtaggcgc aaccatcacc gccagcattg
     5401 ccggatacaa accggcacaa ccgttctgac caatggtcgc accgaaagag gcggcgaaac
     5461 tggcgatgga ttcaggaaca cccagacgac gcgtttgtgc ttccacattc agtgggatag
     5521 acgcagcact ggaacggctg gtaaaggcaa acgtcagtac tggccatacc ttacggaagt
     5581 acttcagcgg gctcacgcca ttaatgccca gcagaaggcc atgcactgca aacataatca
     5641 gcagaccgag gtaggacgcg acaacgaaac ttcccagttt gatgatgtct tgcaggttag
     5701 aacctgcaac cactttggtc atcagcgcca gaacaccgta aggggtcaac tgcatgacca
     5761 ggcgaaccag tttcatcacc cagctttgca gggtatcgat agcggttaag acgcgttcac
     5821 ctttcggcgc atcatccttc agcagtttca gcgcagctac gccgaggaat gcggcaaaaa
     5881 ttaccacgct gataattgac gtcggattgg ctccggtcag atcggcaaac gggtttttcg
     5941 ggataaagga caagaccagc tgcggaacgc tcaggtcaga gactttacca acatagttac
     6001 tttcgatggc gttcagacgt gcagtttctg caccaccctg aaccagacct tcagccgtca
     6061 aaccaaacag gttggtgacc agcacaccaa ccagcgctgc aatcagcgtg gtaaacaaaa
     6121 gcgtaccgat ggtcagaaaa ctgattttgc ctaactgaga tgcgttatgc agacgggcaa
     6181 ccgcgctcag aatagaggcg aagactagcg gcataacgat catttgcagc agttgaacat
     6241 agccgttacc aacaatgtta aaccactgta cagaatcttt aagtacctgg ctgtcagaac
     6301 cataaatggt atgcagggca aggccaaaaa ccacacccat cacaagaccc accagcactt
     6361 ttttcgccag actccactgt ttgtggcggg tttgagccag cgcaaacagc agtacaacga
     6421 acaccacgat gttcgcaatt aatggaaagt tcatccccgt tctcctgatc ttattatttc
     6481 tatcactgcg tagcagtcat agtgttggcg caagattaac agaactgtga gctggctctt
     6541 atattcaaat ggaattgctt attccagaaa ggcgattttt gcctttttag tattcaacct
     6601 gatgcttaat aagccgatga aactggatct gtaaggtatt gatcatttgc gatgaccaac
     6661 gaacagcgct gttttcgggt aatgaatggg gcatccatac cgaccagaca aataatgcca
     6721 tgcagataaa acgttccagt tggttgccct tttggggaac caggataggc aagcggaaac
     6781 gccagcggca tggccagagc aggggaacac cggcgggtgt cagcatatcg gcaagtatgt
     6841 ggctcaaata acccagcacc ataccctgca acgcatcagc cggaatgaac cagctttcag
     6901 gaaccttaag gtaaaaggtt gccagcagcg caaataccgc cagcaggctg tgggtaaaac
     6961 cacgatgccc aaaagcgcgg gcgatcggtt ttgatatcca ctttaatcgc tgcccaagaa
     7021 acgactttgg gtgatcgatg tccggtaaca aacacgtcag gattgcggaa gggacaatat
     7081 gccaccagtc cccctgtgcc agcacgggcg tcagctcggc atttttggca aataccgcac
     7141 aagcaataga aaagagaagg tgaccttccg ccgtcatgat aaaactcact aaactgtcga
     7201 tacgtacagt atagggtttt tatacagtgc acggaagtgg tgacggtgta actctttctc
     7261 ttaaattggc gattaatccg tcatttgaat gcaaattcag ataaagaacc aggggcagtg
     7321 tgatcactgc cccctggtca ttaaccgaga aggtcttttg cggtgagttc tgtcagcgat
     7381 gagagtttga cgtttgctaa tacaaaacgt ggatcatttt gcgcttctgg cgcaggaacg
     7441 acgatggaac gcatgcgggc tgctttagag gcgatcatgc catttaccga atcttccagt
     7501 gccacgcagg tcagagggtc aacccccagt tttgctgcgc agtcaagata tacttgcgga
     7561 tgcggcttgc tgtaaggcag tttttcagcc gaggcgaggg catcgaaact gtcgcgtaaa
     7621 tcaaacatgg tcaatacttt ttccagcata tgcagcggtg acgcggaggc cagccccacc
     7681 aataaacctt gttctttgca taacgcaacg gcttcacgca cacctggcaa taatggacgt
     7741 gtctcttcaa ccagtgaaat ggcacgggca ataacttgtt ctactacttc ctgacggctt
     7801 ggcccattcc atggttgccg ggcgtaccaa agatcgacca ccatatcgat gcgtaaacct
     7861 aaggtgtccg gcagctcgtt acgacgggag atatccaccc ccaggcttgc catcacatcc
     7921 agttcggctc gatcccataa aggttctgag tcgataagta atccatccat atcaaaaatt
     7981 gcagcaagaa tctgacgcgg ggttgacatt acaacctctc ctttagagca ttatatgtaa
     8041 ggtattaagc cgtgcttatg attaagcgcc tatcatatcg cctttaaaga atgggcgaaa
     8101 acagatatct acaggtaaac taagtgaaac gacgcgtctt aatcaaggag aactcatgac
     8161 gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt ttgggttggg tgatttttat
     8221 acctgcattg atctctacat tgatttcttt gctgaaattt atgaataccc ggcaggaaaa
     8281 ccaggaaggc attaatgcgg tcatgctcga ctttactcat gtcatgatcg atatgatgca
     8341 ggcgaatacg ccttttttga atctgttctg gtacaactct ccgacgccca attttaatgg
     8401 tggcgtgaac gttatgttct gggtgatttt catcctgatt tttgtcggac tggcgttgca
     8461 ggattccgga gcccggatga gtcgccaggc gcgcttcttg cgggaaggtg ttgaagatca
     8521 actcattctg gaaaaagcca aaggggaaga ggggctaacg cgtgaacaaa ttgagtcacg
     8581 tattgttgtt ccgcaccata ctattttcct gcagtttttc tcgctgtata tcctgccggt
     8641 tatttgtatt gctgcaggtt atgtgttctt ttctctgctt gggtttattt aatcaaaagg
     8701 ccgcacggag cggccattat cctcaaccca tatcatgctg ctaataatct atccagtgac
     8761 tgctgagcaa taaccaaatg ctgaccgcca aataaccttg cacgatttaa cagcgtgtag
     8821 agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta gcggggatac tgactgatag
     8881 ccgtcataga tttgtggcgg ctgttcagta tgcagcggta acatcgccag gtcgcactct
     8941 cggtcacccc agtaacaggc cgggtcgaaa atgtacgggc catccggacc cagcgcacag
     9001 ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg gttgatgcga ggcaagacgc
     9061 tgctgaatat gctcgacgat ggcatcgata ttgccgaaag cgatcccttt ttctgccgcc
     9121 agttccagct gccagccaat ccgttgttca gcaaaaaaag ttgaccagcg acgttgccag
     9181 gtgttgggct gtggagttgt ggagagcgcg ttatcgaaat cgaggccaaa ttgtggttgg
     9241 tcgctccatt gatgtaaacg cgcaatttgc tgaccaagaa taaatgcact atgcgcatcc
     9301 agcgggcgag gtgggagata atccatcacc agaaaactgt agtcacggtc agcgccaact
     9361 gcccaaacct taggcacggt gacggtttta ctacgcgaca gtaactccag ttggtcggct
     9421 tcggcggtaa aaccgggaag cagttccctt tcatcacatt tgacgaaaaa gtcacgtcct
     9481 gcatagcgca aatgccatgc ggcatggact tctccgccag gcagttcatt acgcagttct
     9541 atttcgcctt cacctaactg ctcgctcaaa agacgactga ttgcctgcca catgatgtct
     9601 ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa tgcggtgtaa aaacacgagc
     9661 taacgcacac cacagactga ttattgccca aaaaccattt attttctgtt gctattccat
     9721 tcctcccagg tcgtgatggt gatttcagga tctttgccgg ttgcactgtg ggtaagcccc
     9781 gaaaccagtt ctctaatttc ttcttcactt tgggtactga taagtccaaa agtgttagtc
     9841 cccagctcat ggatattgcc atcatcatct gtcatggtga gcaaaaaacc gtcgcgagtc
     9901 aggtaattat tcaactcgtt aatttcagtc aatgtatcct catgcaacat gacggttatt
     9961 acataacgga caagatcgcc actggccata gttcacccct ttgttatcac atgacttttg
    10021 agcatagtcg gagaaacgcg ttgccgacag gttggtggtg attcccccaa tgctggggga
    10081 atgtttttgt tagcgggaaa ggtaagcgta aattttttgc gtatcgtcat gggagcacag
    10141 gcgtgttccc tggttgagtg tggctgcact ccccgcagct acgccaaaac ggaccatctc
    10201 ttcaagagaa gcattttctg ccagtttaag tgtcatcgcg ccgaccatgc tgtcaccagc
    10261 gccaacggta ctctggcttt tcaccggtgg tggcaccacc tgaatgcagt tttcactatc
    10321 aacgcccagc gccccttgtg gacccaggga aacgacaacc cgtttggcct tgccgctatt
    10381 aacgatttcc tgcgcggctt tgcggacatc gtctggctgg gtgagttcgc gatttaccag
    10441 cgcactgagt tctttgtggt taggcttaac caactcgatg ttaccaatcg ccagtgctgc
    10501 acttaacgct tcgccagaac tgtcgacgat gcagcggatc ccttgttttt gcgcagcgga
    10561 aatcagttgg gttaattttt ccagcttcac acctggcggc aggcttccgc ttatgaccag
    10621 gatggccccg gattcaattt ccagaacttg ctcttcgagc tggcgaaact catcttcatt
    10681 taatgccgcg cctggcataa caaaacgata ctgctcaccg cttgcttcca catgtacgtg
    10741 taaattctgc cgggtccagt ctttggcttc tacagtagcg acggggacat tttcatccgc
    10801 caacagtgaa accagatgtt cgccggtcgc gccacccgcc gggaagatcg ctgtggcagt
    10861 gcctcccaga tgggcaatgg cgcgggcgac gttgatgccg ccgcctccgg gttcgaatac
    10921 cggtgcggta cagcgcagtt ttccttcggg ataaatttgc ggggtaattg ttgcgctatc
    10981 gagtgagggc gcaagtgtca acgtatagat acgtaccatc atttcctcct ataggctgat
    11041 ttcagtctgg caccgaatca gcggaaagtg aagaaattaa caatatgatt tattggagct
    11101 ttacttaaaa atttgcagat aaatatatat aaataaaaat ctgttttcgc cacatgctta
    11161 ttcaaaaaat gaaataagaa tacattgcta agttattcgc gcctttttat aattcgttac
    11221 ctttccaggg acgctaatct ttatccttaa gaaagtttcc gggaccgcga tggtctcttt
    11281 ttttgttgta cggactcttg ataaatgaag cttttgaaga cagttcctgc catcgttatg
    11341 ttggcggggg gcatgtttgc gtcactgaat gcagctgccg atgattccgt ttttactgtc
    11401 atggacgatc ctgccagcgc taaaaaacct ttcgaaggta atctgaacgc aggatatctt
    11461 gcacaatcag gcaatacgaa aagctcctca cttacggctg acaccactat gacctggtat
    11521 ggacaaacca ctgcctggtc gctgtggggg aacgccagta atacttcttc taatgatgag
    11581 cgttcttcag aaaaatatgc ggcgggcggg cgtagccgtt ttaatttaac tgattatgac
    11641 tatttatttg gtcaggcaag ctggctgaca gaccgttata acggctatcg agagcgcgat
    11701 gtgttgaccg cgggttatgg tcgccagttt ttgaatggcc cggtacacag tttccgcttt
    11761 gaattcggcc ctggtgtgcg ttacgacaaa tataccgaca atgccagtga aacccagccg
    11821 ctgggctatg cctccggggc ttatgcatgg cagttgactg acaacgctaa atttacgcag
    11881 ggtgtttcgg tctttggcgc ggaagacaca acgctgaact ccgaaagcgc attaaatgtt
    11941 gctattaatg aacattttgg actgaaggtg gcttacaacg ttacatggaa ctctgaacct
    12001 ccagagtctg caccggaaca taccgatcgt cggacaacct tatcgttggg ttacagcatg
    12061 taaaattatc tgccggaccg tgttatgcgg tctggctgtt ttcgtttgtc actgctcagc
    12121 cccaaatcat tgctgcccaa cgtagcaaca acatagcgcc acagattgcc agtagcacca
    12181 ccaccatctt ccagtgtttt ttatcaaagc gtttagtcgg cataccagtg tcctgcatat
    12241 ctcttgtgcg tgataacgga gtgtaacaaa agggacaggc atcaagggat tttttgttat
    12301 tacagtcgtc gggtggcgtt gtacacctgg tcactgtttc tgcattttat gaaaaaaatt
    12361 atgtatcgat atacaaatat cataaatagc gggttataaa tacgtcttta gttcatcagc
    12421 actaaaactc aggctttggc tgattacatt tatagcagca taatatgttt ctacagtttt
    12481 atgatgatta tgatgaatgg ctatatgaac agctgacatg ttgtcatgat ttttagcggc
    12541 caacagtgta aataactgac gttggctaaa agaatatttt tttcaaaagt acccaacgtt
    12601 gatatataag ataacacgac atctttactt actgacaaag tatgattgtc tatatataaa
    12661 atcgcggaca tatgactgac attgtttgcc atcattcgac agaagaaaat atctaaagca
    12721 aagccaaaat cctgactcgc gataacgcga ctccttgcaa gaggatggcg aagtaatgct
    12781 ctgaattccg cgtcccggac taagggaaca tgctgctgta tggattcaat ggaggagtaa
    12841 tgtttaatca ggttgacttt gccttttcca tttctcaatg aatcaagggc gtattgtaat
    12901 gacggatggc gacaaaaaat agtgtcagaa ggagattgcg aaaaaataca ctatcattga
    12961 gatttactgt tggctgtatg gttttttctc tatgaatttt tttggacagt ttcatgcaat
    13021 ggttacgttt ttcggcagtt aactgttccc gttcaatata atccagtatg cttcgcaaat
    13081 ggttagcttt aataggcgtg gtttccagaa ataaggaaat gatttatttt ttatagtgta
    13141 atgagtgtat tttctgcatg gatgggtgaa gtggcaaagt taatgataaa aagggcagtg
    13201 gaataacaat tgtgtgatat ttttcgacag cttacggtaa attcctgagt aacacagtat
    13261 gacagcatcg aacttaaatc agaggtatca agtagggtga tgagctggcg cgcaaattga
    13321 ctaataggct ctgatgaagg cgttagtatg tcgctatcga tgtgcattct aagaggagac
    13381 tcataatgag ttctgataat gatatcgttt tcactcataa tctattcgcc ttatatatgt
    13441 ctggagattt tgaggatgat gattaattat tatcgccaga acgaaatgcg cacgaaaaat
    13501 tgctataaga attgtgtcac ttttatcatt gcgatatgac cacttcgtca ctgaatctca
    13561 tgatcgttta aataccaccg ccaaataacg caacgaactt tgctggattc agtttatgct
    13621 gctgtaaatc cgctcgtagt aaacctttca gacactctgt gatgatatca gtggttcttt
    13681 aagcgttttg ctggtgtact cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga
    13741 aaggtacaat ctcccttttt ttagtgttaa catcgttcaa ccgggtggaa ggtgagaggt
    13801 taatgtcttt catttgtgta cctgattgcg aaccaattta gcatttgttg gctagatggt
    13861 ttcgcaatga actgttaata aacaaatttt tctttgtatg tgatctttcg tgtgggtcac
    13921 cactgcaaat aaggatatat aatgcctgtt ataactcttc ctgatggcag ccaacgccat
    13981 tacgaccacg ctgtaagccc catggatgtt gcgctggaca ttggtccagg tctggcgaaa
    14041 gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg cttgcgatct gattgaaaac
    14101 gacgcacaac tgtcgatcat taccgccaaa gacgaagaag gtctggagat cattcgtcac
    14161 tcctgtgcgc acctgttagg gcacgcgatt aaacaacttt ggccgcatac caaaatggca
    14221 atcggcccgg ttattgacaa cggtttttat tacgacgttg atcttgaccg cacgttaacc
    14281 caggaagatg tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa aaactacgac
    14341 gtcattaaga agaaagtcag ctggcatgaa gcgcgtgaaa ctttcgccaa ccgtggggag
    14401 agctacaaag tctccattct tgacgaaaac atcgcccatg atgacaagcc aggtctgtac
    14461 ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc
    14521 catcatttca aactaatgaa aacggcaggg gcttactggc gtggcgacag caacaacaaa
    14581 atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg
    14641 caacgcctgg aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg
    14701 taccatatgc aggaagaagc gccgggtatg gtattctggc acaacgacgg ctggaccatc
    14761 ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag agtaccagta tcaggaagtt
    14821 aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac
    14881 aaagatgcaa tgttcaccac gtcttctgag aaccgtgaat actgcattaa gccgatgaac
    14941 tgcccggggc acgtacaaat tttcaaccag gggctgaagt cttatcgcga tctgccgctg
    15001 cgtatggccg agtttggtag ctgccaccgt aacgagccgt caggttcgct gcatggcctg
    15061 atgcgcgtgc gtggttttac ccaggatgac gcgcatatct tctgtactga agaacaaatt
    15121 cgcgatgaag ttaacggatg tatccgttta gtctatgata tgtacagcac ttttggcttc
    15181 gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac gtattggcag cgacgaaatg
    15241 tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag aaaacaacat cccgtttgaa
    15301 tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg aatttaccct gtatgactgc
    15361 ctcgatcgtg catggcagtg cggtacagta cagctggact tctccttgcc gtctcgtctg
    15421 agcgcctcct atgtgggcga agacaacgag cgtaaggttc cggtaatgat tcaccgcgca
    15481 attctggggt cgatggaacg tttcatcggt atcctgaccg aagaattcgc tggtttcttc
    15541 ccgacctggc ttgcgccggt tcaggttgtt atcatgaata ttaccgattc acagtctgat
    15601 tacgttaacg aattgacgca aaaactatca aatgcgggca ttcgtgttaa agcagacttg
    15661 agaaatgaga agattggctt taaaatccgc gagcacactt tgcgtcgcgt cccatatatg
    15721 ctggtctgtg gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt
    15781 aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc
    15841 agccgcagtc ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg agttcaaacg
    15901 gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg
    15961 gaaggcgagc agcttggtat tgtgagtctg agagaagctc tggagaaagc agaagaagcc
    16021 ggagtagact tagtcgagat cagccctaac gccgagccgc cggtttgtcg tataatggat
    16081 tacggcaaat tcctctatga aaagagtaag tcttctaagg aacagaagaa aaagcaaaaa
    16141 gttatccagg ttaaggaaat taaattccgt cctggtacag atgaaggcga ctatcaggta
    16201 aaactccgca gcctgattcg ctttctcgaa gagggtgata aagccaaaat cacgctgcgt
    16261 ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa
    16321 gacgatttgc aagaactggc agtggtcgaa tccttcccaa cgaagatcga aggccgccag
    16381 atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg
    16441 gagcctttgg gcttcacagg cattgttcgc ctttgtttcg tttattaaca atgcgaagtg
    16501 gaagttatta aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa
    16561 accggtaaag gtggttttaa gcacaagcac gctaacctgc gtcacattct gaccaaaaaa
    16621 gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg cgatctgggc
    16681 ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg tttttaactt tttaattaga
    16741 atatagatac aggagagcac atatggctcg cgtaaaacgt ggtgttattg cacgcgcacg
    16801 tcacaagaaa attttgaaac aagctaaagg ctactacggt gcgcgttctc gcgtataccg
    16861 cgttgccttc caggctgtta tcaaagctgg tcagtatgct taccgcgacc gtcgtcaacg
    16921 taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac gcagcagcac gtcagaatgg
    16981 tatttcttac agcaaattca tcaatggcct gaaaaaagcc tctgttgaaa tcgaccgtaa
    17041 gatcctggct gatatcgcag tattcgacaa agtagcgttc accgctctgg ttgaaaaagc
    17101 gaaagcagct ctggcataag ccagttgaaa gagggagcta gtctccctct tttcgtttca
    17161 acgccatcaa aacattgact tttatcgccg tagccttttc aataaaggtc ttttgaagag
    17221 taccaaaagg taacgcaagc aatgaatgct gctattttcc gcttcttttt ttactttagc
    17281 acctgactcc aggaggctag cgcgtgagaa gagaaacgga aaacagcgcc tgaaagcctc
    17341 ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa aaagcctgtc tcatcaggcg
    17401 taattgtcgc agccagtctg gacacggcca gcgcggagaa accggaccgt acttaagtac
    17461 gtgaggattt cgagcactgc ccgggaccaa aatggcaagt aaaatagcct gatgggatgg
    17521 gctctaagtc caacgaacca gtgtcaccac tgacacaatg aggaaaaaca tgtcacatct
    17581 cgcagaactg gttgccagtg cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt
    17641 agataatgtg cgcgtcgaat atttgggtaa aaaagggcac ttaacccttc agatgacgac
    17701 cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt gcggttatca atgaagcgaa
    17761 agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa ctggaaagcg cagcactgaa
    17821 tgcgcgtctg gcggcggaaa cgattgatgt ctccctgcct ggtcgtcgca ttgaaaacgg
    17881 cgggctgcat ccggttaccc gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg
    17941 ctttaccgtg gcaaccgggc cggaaatcga agacgattat cataacttcg atgctctgaa
    18001 cattcctggt caccaccctg cgcgcgctga ccacgacact ttctggtttg acgctacccg
    18061 cctgctgcgt acccagacct ctggcgtaca gatccgcacc atgaaagccc agcagccacc
    18121 gattcgtatc atcgcgcctg gccgcgttta tcgtaacgac tacgaccaga ctcacacgcc
    18181 gatgttccat cagatggaag gtctgattgt tgataccaac atcagcttta ctaacctgaa
    18241 aggcacgctg cacgacttcc tgcgtaactt ctttgaggaa gatttgcaga tccgcttccg
    18301 tccttcctac ttcccgttta ccgaaccttc tgcagaagtg gacgtcatgg gtaaaaacgg
    18361 aaaatggctg gaagtactgg gctgcgggat ggtgcatccg aacgtgctgc gtaacgttgg
    18421 catcgacccg gaagtttact ctggtttcgc cttcgggatg gggatggagc gtctgactat
    18481 gttgcgctac ggcgtcaccg acctgcgttc attctttgaa aacgatctgc gtttcctcaa
    18541 acagtttaaa taaggcagga atagattatg aaattcagtg aactgtggtt acgcgaatgg
    18601 gtgaacccgg cgattgatag cgacgcgctg gcaaatcaaa tcacgatggc gggcctggaa
    18661 gttgatggtg tagaaccggt tgccggtagc ttccacggcg tggttgtggg tgaagtggtt
    18721 gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcggc
    18781 gatcgcctgc tggacatcgt ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg
    18841 gtagcgacca ttggtgctgt tctgccgggt gatttcaaaa ttaaagccgc gaaactgcgt
    18901 ggcgaaccgt ctgaagggat gctgtgctcc ttctctgagc tgggaatttc tgacgatcat
    18961 aacggcatta tcgagctgcc tgcggatgcg ccgattggca ccgacatccg cgaatacctg
    19021 aaactcgatg acaacaccat cgaaatcagc gtgacaccaa accgtgccga ctgcttaggt
    19081 atcattggtg ttgcgcgcga cgttgccgtg ttgaaccagc tgccgctggt tgaaccggaa
    19141 atcgttccgg ttggtgcgac catcgacgac acgctgccga ttgcagtaga agcgccggat
    19201 gcctgcccgc gctatcttgg ccgtgttgta aaaggcatta acgttaaagc gccaactccg
    19261 ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac
    19321 gtcaccaact atgtgctgct cgaactgggc cagccgatgc acgctttcga taaagatcgc
    19381 attgaaggcg gcattgtggt gcggatggca aaagagggtg aaacgctggt attgctcgac
    19441 ggtactgaag cgaagctgaa tgctgacacc ctggtcatcg ccgaccacaa caaggcgctg
    19501 gcgatgggcg gcatatttgg tggcgaacac tctggcgtga atgacgaaac gcaaaacgtt
    19561 ctgctggaat gcgctttctt cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc
    19621 ctgcatactg atgcgtctca ccgttatgag cgtggcgttg atccggcact gcagcacaaa
    19681 gcgatggaac gtgcgacccg tctgctgatc gacatctgcg gtggtgaagc gggtccggta
    19741 attgatatca ctaacgaagc aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc
    19801 aaactggatc gcctgatcgg ccatcatatt gctgatgagc aggtaacaga cattctgcgt
    19861 cgtctcggct gcgaagtgac cgaaggcaaa gacgagtggc aggcagttgc gccgagctgg
    19921 cgtttcgaca tggagattga agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac
    19981 aacaacatcc cggatgagcc ggtacaggca agcctgatta tggggattca ccgtgaagct
    20041 gacctgtcgc tcaagcgcgt aaaaacgctg ctcaacgaca aaggctatca ggaagtgatc
    20101 acctacagct tcgttgatcc gaaagtgcag cagatgatcc atccaggcgt tgaagcctta
    20161 ctgctgccaa gcccgatctc tgttgaaatg tcagcaatgc gtctttctct gtggaccggc
    20221 ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga atcgtgtacg cattttcgaa
    20281 agcggtctgc gtttcgtgcc agatactcag gcaccgttgg gtattcgtca ggatctgatg
    20341 ttagccggtg tgatttgcgg taaccgttac gaagagcact ggaacctggc aaaagagacc
    20401 gttgatttct atgatttgaa aggcgatctt gaatccgttc tcgacctgac cggtaaactg
    20461 aatgaggttg agttccgtgc agaagcgaat ccggcactgc atccggggca atccgcagcg
    20521 atttatctga aaggtgaacg tattggtttt gttggggttg ttcatcctga actggaacgt
    20581 aaactggatc ttaacggtcg cactctggtg ttcgaactgg agtggaacaa gctcgcagac
    20641 cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg
    20701 gtggtggtcg cagaaaacgt tcccgcagcg gatattttat ccgaatgtaa gaaagttggc
    20761 gtaaatcagg tagttggcgt aaacttattt gacgtgtacc gcggtaaggg tgttgcggag
    20821 gggtataaga gcctcgccat aagcctgatc ctgcaagata ccagccgtac actcgaagaa
    20881 gaggagattg ccgctaccgt cgccaaatgt gtagaggcat taaaagagcg attccaggca
    20941 tcattgaggg attgaaccta tggcgcttac aaaagctgaa atgtcagaat atctgtttga
    21001 taagcttggg cttagcaagc gggatgccaa agaactggtt gaactgtttt tcgaagagat
    21061 ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct ggttttggta acttcgatct
    21121 gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg ggcgaggata ttcccattac
    21181 agcacggcgc gtggtgacc
//
LOCUS       DAOFSV010000072        21171 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.072, whole genome shotgun sequence.
ACCESSION   DAOFSV010000072 DAOFSV010000000
VERSION     DAOFSV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.072"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            120..1232
                     /gene="potF"
                     /locus_tag="QBH27_002790"
     CDS             120..1232
                     /gene="potF"
                     /locus_tag="QBH27_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDQ7015084.1"
                     /translation="MIALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            1327..2460
                     /gene="potG"
                     /locus_tag="QBH27_002791"
     CDS             1327..2460
                     /gene="potG"
                     /locus_tag="QBH27_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDQ7015085.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            2470..3414
                     /gene="potH"
                     /locus_tag="QBH27_002792"
     CDS             2470..3414
                     /gene="potH"
                     /locus_tag="QBH27_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDQ7015086.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIVMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            3411..4256
                     /gene="potI"
                     /locus_tag="QBH27_002793"
     CDS             3411..4256
                     /gene="potI"
                     /locus_tag="QBH27_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDQ7015087.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            4316..4804
                     /locus_tag="QBH27_002794"
     CDS             4316..4804
                     /locus_tag="QBH27_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDQ7015088.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            4845..5972
                     /gene="rlmC"
                     /locus_tag="QBH27_002795"
     CDS             4845..5972
                     /gene="rlmC"
                     /locus_tag="QBH27_002795"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDQ7015089.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(6001..6732)
                     /gene="artJ"
                     /locus_tag="QBH27_002796"
     CDS             complement(6001..6732)
                     /gene="artJ"
                     /locus_tag="QBH27_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDQ7015090.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(6958..7626)
                     /gene="artM"
                     /locus_tag="QBH27_002797"
     CDS             complement(6958..7626)
                     /gene="artM"
                     /locus_tag="QBH27_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDQ7015091.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(7626..8342)
                     /gene="artQ"
                     /locus_tag="QBH27_002798"
     CDS             complement(7626..8342)
                     /gene="artQ"
                     /locus_tag="QBH27_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDQ7015092.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(8349..9080)
                     /gene="artJ"
                     /locus_tag="QBH27_002799"
     CDS             complement(8349..9080)
                     /gene="artJ"
                     /locus_tag="QBH27_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7015093.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(9098..9826)
                     /gene="artP"
                     /locus_tag="QBH27_002800"
     CDS             complement(9098..9826)
                     /gene="artP"
                     /locus_tag="QBH27_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDQ7015094.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(10044..10559)
                     /locus_tag="QBH27_002801"
     CDS             complement(10044..10559)
                     /locus_tag="QBH27_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ7015095.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            10685..11008
                     /locus_tag="QBH27_002802"
     CDS             10685..11008
                     /locus_tag="QBH27_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDQ7015096.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            11005..11835
                     /gene="amiD"
                     /locus_tag="QBH27_002803"
     CDS             11005..11835
                     /gene="amiD"
                     /locus_tag="QBH27_002803"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDQ7015097.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(11832..12845)
                     /locus_tag="QBH27_002804"
     CDS             complement(11832..12845)
                     /locus_tag="QBH27_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ7015098.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(12944..14374)
                     /locus_tag="QBH27_002805"
     CDS             complement(12944..14374)
                     /locus_tag="QBH27_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDQ7015099.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPVLLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQVIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(14385..15386)
                     /gene="ltaE"
                     /locus_tag="QBH27_002806"
     CDS             complement(14385..15386)
                     /gene="ltaE"
                     /locus_tag="QBH27_002806"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDQ7015100.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     EFSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(15423..17141)
                     /gene="poxB"
                     /locus_tag="QBH27_002807"
     CDS             complement(15423..17141)
                     /gene="poxB"
                     /locus_tag="QBH27_002807"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDQ7015101.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(17274..18242)
                     /gene="hcr"
                     /locus_tag="QBH27_002808"
     CDS             complement(17274..18242)
                     /gene="hcr"
                     /locus_tag="QBH27_002808"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDQ7015102.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVFA"
     gene            complement(18254..19906)
                     /gene="hcp"
                     /locus_tag="QBH27_002809"
                     /gene_synonym="priS"
     CDS             complement(18254..19906)
                     /gene="hcp"
                     /locus_tag="QBH27_002809"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDQ7015103.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIIGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(20050..20949)
                     /gene="lysO"
                     /locus_tag="QBH27_002810"
     CDS             complement(20050..20949)
                     /gene="lysO"
                     /locus_tag="QBH27_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDQ7015104.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSTTVILLCNIAALMWLERGLPWSNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         5013 a         5444 c         5650 g         5064 t
ORIGIN      
        1 ggccgggaga gagttctctt ttcttacacc gcgccgataa aaatatgcac gtttattgca
       61 tatctttcag tgtgacaact tttgttcgtt tgttaacgaa ttttcagaag gaaagagata
      121 tgatcgcctt aaataaaaaa tggctatcgg gtctggttgc gggtgctctg atggccgttt
      181 ctgtcagcac gctcgcggct gaacaaaaaa cactccacat ttataactgg tctgattata
      241 tcgccccgga cacggtggcc aactttgaaa aagaaaccgg tattaaagtc gtctatgatg
      301 ttttcgattc taacgaagta ctggaaggca aattaatggc ggggagcacc ggctttgatc
      361 tggtggtgcc gtcagcaagc tttcttgagc gccagctgac agcgggagtt ttccagccgc
      421 tggataaaag caaattgccg gagtggaaga atctcgatcc ggagctgctg aagttggtcg
      481 ccaaacacga tcccgacaat aaatttgcta tgccctatat gtgggcgacg accgggattg
      541 gctataacgt cgataaagtt aaagcggtgc tgggcgaaaa cgcgcccgtc gatagctggg
      601 acttgatcct caaacctgaa aatctggaaa aactgaaaag ctgcggtgtc tctttcctgg
      661 atgcgccaga agaagtgttt gctaccgtgc tgaattatct cggcaaagat cctaacagca
      721 ctaaagcgga tgattacacc ggaccggcta ccgatctgct gttaaagctg cgcccgaaca
      781 ttcgttattt ccattcatcc caatacatca atgatctggc gaatggtgac atctgtgtcg
      841 ctattggctg ggcgggcgat gtctggcagg cgtcaaaccg cgcgaaggaa gcgaagaatg
      901 gcgtgaatgt ctcgttctcg attccaaaag aaggggcgat ggcgttcttt gatgtattcg
      961 ccatgcctgc ggatgccaaa aacaaagacg aagcctatca gttcctgaat tacctgctgc
     1021 gtcctgatgt ggtggcacat atctctgacc atgtgttcta tgctaacgcc aataaagcag
     1081 ccacgccgct ggtgagtgcg gaagtccgtg ataacccggg tatttatccg cctgcggatg
     1141 ttcgtgcgaa gctgttcact ctgaaagtgc aggacccgaa aatcgaccgt gtgcgcaccc
     1201 gcgcctggac caaagtgaag agcggaaaat aatccgcagc cgtagatgcc ggaggggcgc
     1261 accacacccg ccggcaattc gcaccatcat ggtgcgcttg cacacattca atgccggaga
     1321 gcagccgtga atgacgctat ccctcgcccg caggcgaaaa cccgtaaggc gctgacgccg
     1381 ctattagaaa tccgtaacct gaccaaatcc tacgatggtc aacatgcggt ggatgatgtc
     1441 agcctgacca tctacaaagg tgaaatcttc gcgctgctgg gcgcatccgg ctgtggcaag
     1501 tccacgctgc tgcgtatgct ggcaggtttt gaacaacctt cggccgggca gattatgctc
     1561 gatggcgtcg atttgtcaca ggtgccgcct tacctgcgcc ccatcaatat gatgtttcag
     1621 tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg cctgaaacag
     1681 gacaaactac cgaaagcgga aattgccagc cgggtcaatg agatgcttgg gctggtgcat
     1741 atgcaggagt tcgccaaacg caaaccgcat cagctttctg gtggtcagcg tcagcgtgtg
     1801 gccctggcca gaagccttgc caagcgcccg aaactattac tgctcgatga gccgatgggc
     1861 gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat tctggagcgc
     1921 gtcggtgtga cctgcgtaat ggttactcac gatcaggaag aggcgatgac tatggcgggg
     1981 cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgagccgga agagatctac
     2041 gagcatccga ccactcgcta cagtgctgaa tttatcggtt cggttaatgt ctttgaaggt
     2101 gtactcaaag agcgtcagga agatggtctg gtgctttatt cgccggggct ggtgcatcca
     2161 ctgaaagtcg atgcggatgc ttcagtggtc gataacgtgc cggtgcatgt ggcgcttcgc
     2221 ccggaaaaaa tcatgctttg cgaggaaccg cccgccaatg gttgtaactt cgcggtgggg
     2281 gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg tctgaaaagt
     2341 gggcagatga ttagcgccca gctacaaaac gcccatcgtc atcgtaaagg gttaccgacc
     2401 tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct gacggtttaa
     2461 ggagcaaaga tgagtacact tgaacctgct gcccagccaa aaccctttaa gctgtggttg
     2521 tcgcagctgc aaatgaaaca tgggcgcaaa ctggttattg cgttgccgta tatctggttg
     2581 atcttgctgt ttctgctgcc atttctgatc gtctttaaaa taagcctggc ggagatggcg
     2641 cgcgctattc caccttatac cgaattgatg gagtgggctg acgggcaact ttctatcact
     2701 cttaatctcg gtaattttct gcaactgacc gacgatccgc tctattttga tgcttatctc
     2761 cagtcgttac aggtggcggc gatttcgact atttgctgtt tactgatcgg ctatccgctg
     2821 gcgtgggcgg tggcgcacag taagccttcg acccgtaata tcttattact actggtgatc
     2881 ctgccgtcgt ggacctcgtt tctgattcgt gtctacgcct ggatgggaat attaaaaaac
     2941 aacggtgtgc tgaataattt tctgctgtgg ctggggatta tcgatcaacc gctgaccatt
     3001 ctgcatacca actttgcggt ttatattggc attgtttacg cctacgtgcc gtttatggtg
     3061 ctgccgattt acaccgcgtt gattcgtatt gattattcgc tggtagaagc ggcactggat
     3121 ctcggtgcac gaccgctgaa aacgttcttt actgtgatcg tgccgctgac taaaggtggg
     3181 attattgccg gatcgatgct ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa
     3241 ctgctcggtg gcccggacag catcatgatc gggcgcgtgc tgtggcagga gttctttaac
     3301 aaccgcgact ggccggtggc ctcagcggta gcgatcgtca tgttgctgct gctaattatg
     3361 ccgataatgt ggttccacaa acatcagcaa aaaagcgagg gagaacacgg atgaataatt
     3421 taccggtggt tcgttcgccc tggcggattg tgattttgct gttgggcttc acctttctct
     3481 acgcgccaat gctgatgctt gttatctatt cgtttaacag ctcgaagcta gtgacggtgt
     3541 gggccggctg gtcaacgcgc tggtatggcg aattattgcg cgatgacgcg atgatgagtg
     3601 cggttgcttt aagtctgaca attgcggcct gtgcggcgac ggcggcggcg atcctcggga
     3661 ctattgcggc ggtcgtgctg gtgcgctttg gcaggtttcg cggatcaaat ggttttgcct
     3721 tcatgatcac cgcaccgctg gtaatgccag atgtcatcac gggtttgtcg ctgttgttgt
     3781 tattcgtcgc gcttgctcat gctattggct ggcctgcgga ccgcggtatg ctcaccatct
     3841 ggctggcgca tgtcactttc tgtacggctt atgtggcagt cgttatttcg tcgcgtctgc
     3901 gggaactgga tcgctcgata gaagaagccg cgatggatct cggtgcgacg ccgctgaaag
     3961 tgttttttgt cattacgcta ccgatgatca tgcccgcgat catttctggc tggttactgg
     4021 cttttacttt gtcgcttgat gatctggtga tcgccagctt tgtttccggg ccgggagcca
     4081 ccacattacc gatgctggtc ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg
     4141 ccctggcaac attgatcctc ggcgcggtcg gaattgtcgg atttatcgcc tggtatctga
     4201 tggcgcgcgc agaaaaacag cggatacgcg acatccagcg tgcaagacgt ggctgaagac
     4261 actaaaattt gccaacctgg ctacataatg ccgcgcatgt cgcggcattg ttttcatgga
     4321 agacgaaacg ttgggatttt ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc
     4381 tgcgctggtg caggtggcgg cgctcgccat tattatgatc cgtggcctcg acgtgctgat
     4441 gattttcaat acgctgggcg tgcgcggtat tggcgagttc attcatcgca gcgtacaaac
     4501 ctggagttta acgctggtct ttttgagcag tctggtgctg gttttcattg agatctggtg
     4561 tgcgttttca ctggtgaaag ggcgtcgctg ggcgcgctgg ctgtatctgt tgacacaaat
     4621 cacagccgca agttacttgt gggcggcttc gctggggtat ggttatccgg agctgttcag
     4681 cattcccggt gaatcaaaac gtgaaatctt ccatagcctg atgctgcaga agctgccgga
     4741 tatgctcatc ctgatgctgc tgttcgttcc ctcgaccagt cggcggttct tccagttgca
     4801 ataatgtgta taatcgtcgc ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt
     4861 acgacgcggg tcgctgtcgt tcctgtcagt ggataacgca gccgattcca gagcaactct
     4921 ccgctaaaac tgccgatctt aaaaatctgc tcgccgattt tccggttgag gaatggtgtg
     4981 cgccggtgtc aggcccggaa caagggtttc gtaataaagc caaaatggtg gtgagtggta
     5041 gcgttgaaaa accactgctc ggtatgctgc atcgagatgg tacaccggaa gacctttgtg
     5101 actgcccgct ttatccagcc tcatttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg
     5161 cccgtgcggg gttaacgccc tacaacgtgg cgcgtaaacg tggtgaactg aaatacattc
     5221 tgctgactga aagccagagc gatggtggca tgatgctgcg ttttgtactg cgttctgata
     5281 ccaaactggc gcaattgcgt aaggcgctgc cgtggttaca ggaacaatta ccgcagctga
     5341 aagttattac cgtcaatatt cagccggtac atatggcgat tatggaaggg gagacggaga
     5401 tctacctgac cgaacaacag gcactggcag agcgttttaa tgacgtaccg ctgtggatcc
     5461 gtccgcaaag tttcttccag actaatccgg cggtcgccag ccagctgtac gccaccgcgc
     5521 gcgactgggt acgacagctg ccggttaaac atatgtggga tctgttctgc ggtgtgggtg
     5581 gctttggttt acactgcgcg acgcctgaca tgcagttaac cgggatcgaa attgcaccag
     5641 aggccattgc ctgtgcgaag cagtcagccg ctgaactggg cttaacgcgt ttgcaatttc
     5701 aggcgctgga ctctacgcag tttgccaccg ctcaggggga agtgccggag ctggtgctgg
     5761 ttaacccgcc gcgccgcggc atcggtaaac cgctgtgtga ttatctctca acgatggcac
     5821 cgcgttttat tatctactcc agctgtaacg cccaaactat ggcgaaagat atccgcgaac
     5881 tgcctggtta tcgcatcgaa cgggtacagc tttttgatat gttcccgcac accgcgcact
     5941 atgaagtgct gacgctactg gtgaagcaat aaaaaagccg caggtgcggc tttctgaatc
     6001 ttactgtggg aaccactggt cattgatttt ctgatatgta ccgtcagctt taattgctgc
     6061 cagcgcgtta ttcagttttt ccagcagagc tttgttatcc ggacgtacag cgatacccag
     6121 gccggtgccg aagtattgcg gatcggtcac tttttcagta gcaacgccca gttgcggatt
     6181 ggttttcagc cattcgttta ccactgcggt gtcaccaaat acgccatcaa tacggccatt
     6241 tttcagatcg ataaaggcat tctgataact gtcataggag acagttttca cttccgggtg
     6301 ctgatcctga atatatttct ggtgcgtggt gccgttttcc atcccaatac gtttgccttt
     6361 cagatcggca aacgttttgt aggtatcttt tttggcaatc acgacggctg agttttcgta
     6421 gtagggcgtg gtaaacgata cctgtttgct acgctccggc gtgatatcca ttccggagat
     6481 tacggcgtca tattttctga atttcagtga cgggatcagg ctgtcgaaag cgtgattagt
     6541 aaaagtacat tctgcctgca tttgtttgca caaggctttt gccaggtcga tatcaaagcc
     6601 gacaatctca ttattagcac ctatagattc aaagggtgga taggtggctg aaacgccaaa
     6661 attgattttc tctgcggcag aagcaccgaa agtaaaagaa gcaagtaaag cggcaagaac
     6721 taactttttc atgatggaac tcccgtctgt caatcttatg atttttggcc gtgtctgcgg
     6781 catgggataa caatgccatt aagtgaattt atatgcaata aatatgagta aataatttat
     6841 atgaaataaa aaaagacgga cagcttagtg ggttgtccgt cttcataata agaatttatg
     6901 cactatgtag gccggataag gcgtccacgc cgcatccggc atcagcatgc agctacatta
     6961 attccgccgt tcgaatgcca gtgctttacg ctcgatcaga cgcatcatca gcgtcagcag
     7021 gccgttgacg accaggtaaa taatccccgc cgcgccaaat accattacat cgtaggtgcg
     7081 tccgtacaac aactggctgt atcccatcac ttccatgagc gtaatggtgt aagccagaga
     7141 ggtacttttg aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaacgc
     7201 gtacggcagc aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg
     7261 ccattgacct tccgggatcg cgcgaatcgc accgtaaaac agctgcgtgg tgtacgccgc
     7321 actgttcagc gacaacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag
     7381 tgccggatac tcctgcaacg tcgggaactg gcccggcccg taataaatca ggaagatctg
     7441 caccagcagc ggcgtaccgg taaacagcgt gatatagccc cgtaccagcc acaccagcac
     7501 cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag
     7561 cgaggcaacg gttagcgtca ggctggtgtg cagccctttc atcagttcgg gtaaatactc
     7621 aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg
     7681 tactgactga gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaac
     7741 ggttcctggg tacgggtagc gatgcttttg gtttgtagca ttaaatcatt cacactaatc
     7801 aaactgacca gcgcggtatc tttcagcagc accagccact ggttaccgag acccggcagc
     7861 gcatgacgcc acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc
     7921 agcgcctgac cggattccca ctgccccacc ggcaccgctt ttaatgcacc acgcagcgtt
     7981 tgcgaggcat aggcggcata cagcagtgac agagcgatga caccacaaag gaacgggcta
     8041 acgtcgaagt tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg
     8101 aagccatccg aaagcgtcag cagcagctgc gaggagccaa aatagataaa cagcaccacc
     8161 agaatttctg gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga
     8221 cgccatttcg cagactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat
     8281 gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc
     8341 atcaggaatt acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc
     8401 actttttcca gcgcagtgtt gaatttctgc tgcagttcag tgttgccctg gcgtactgcg
     8461 atgccgaggc cagtaccgaa gtaatcttta tcggtcactt tatcgcccac tgccgccagt
     8521 ttcgggttat ctttcagcca ttcagttacc actgcggtgt caccaaatac gctgtcgata
     8581 cgaccgtttt gcagatccag ttttgcgttc tggtaactgt catatggcac ggtagtgatt
     8641 tccggatgct tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccaactttt
     8701 ttgcctttca gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag
     8761 ttgtcatagt acggggtggt aaacagcacc tgcttttcac gctccggagt gatatccatg
     8821 cccgccatca ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc
     8881 tggttagaga aagtgcaggt tgcatcaatc tctttacaca atgcctgcgc caggtcgaca
     8941 tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa acggaggata ggaggcttcg
     9001 gtggcaaaac gaatggtttc ggctgctgtg gcggaaagac taaaacctgc aattaacgcg
     9061 gcaatcagaa cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa
     9121 atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccgt
     9181 tttccatata caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga
     9241 tcacctgggt aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa
     9301 tttcggggtc cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca
     9361 acgcacgggc aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat
     9421 cgctataagg tttaagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat
     9481 ctttactcag ccccagtaca cggcagggcg cttcaatcag gttttgctgc acggtcagat
     9541 gcggccacag gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg
     9601 ctttgtcaga gggggttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg
     9661 agcgcggcat ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg
     9721 ggccaagtaa caccagcgtt tcgccctgcg ggcaatccag cgtgatatcg aacagcgcct
     9781 gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac
     9841 tggcagtctg atagctattg aggtcgaaga tagtaccttt gacagaataa ttatgcaata
     9901 tttctgcttt aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg
     9961 ggggcggtgg tcggcgagca taaatgtcgg cattcctcac gaaatgccgg acaatttacg
    10021 gggtttattg gttgatcaag acgttagcga ttctcgatgg attgacggag cgtacccgcc
    10081 gtggcatgaa cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc
    10141 tgaatcatta acacttcatc ctgccagccc tggtcaccct gcttgagatc cacgcgcaat
    10201 ggaatgttac gggcatcacg attagggata gtcgatgcac tggctacgtg ggcgctgtct
    10261 ggcaaggtgg ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg
    10321 cggctggcat cgctaagtag ggttgccagt ttgtcactca aataggggcg cagagcggta
    10381 atgtcattgc tgcgatgttg aatgcggtag tcatagaact gttgagccac actatcaggc
    10441 cccccctgga cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg
    10501 gtacaggcac tgagtagcag tgcgcaaggg ataagcatgg tcaatttgct atagcgcatg
    10561 atgatttcct tataagcgaa caccttgaaa gcgttctacg ataataatga tatcctttca
    10621 ataatagcgt atcagtccga taatgctttg aagatcgaag gcttagcaaa caaggagatc
    10681 gatcatgcaa ttttctacaa ccccaactct ggaaggccag accatcgttg aatattgcgg
    10741 tgtggtgact ggcgaagcga ttttaggcgc gaatattttc cgtgatttct ttgccggtat
    10801 ccgcgatatc gttggcggac gctccggtgc ctacgaaaaa gaactgcgta aagcgcggga
    10861 gattgccttt gaggagctcg gctcccaggc gcgagcgctg ggggctgatg ccgtcgtcgg
    10921 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    10981 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    11041 ttactggcag ggtgcacagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    11101 acccgacgcc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    11161 gcagatgatt ttgatagctc gctggcgaca ctgaccgata agcaggtcag ctcgcattat
    11221 ctggtccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    11281 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgtcttaac
    11341 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcggc gggagtgaaa
    11401 tattttgccc cgtttgaacc ggcacagatt caggcgctta ttccgctggc gaaagatatt
    11461 attgcccgtt atcacatcaa gccggaaaac gtagtagcac atgcggatat tgcgccacag
    11521 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcacagca ggggattggt
    11581 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    11641 gtagagaccg cgtcattgct ggagcttttg gcgcgttatg gttatgacgt aaagcctgat
    11701 atgactccgc gcgagcagcg gcgtgtgatt atggcgttcc agatgcattt ccgcccgacg
    11761 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcgtt gctggagaaa
    11821 tacgggcagg attagcgcgg cagttttccg tggtcgcgca gccaggcggc agttttctcg
    11881 ataccttcat ccagggtgag gactggctga taacctaact cttcctgcgc acgcgtaata
    11941 tccagcgtaa agtcaaaatt caacttggag acgccgtagt gggtcagcgg cggctctttt
    12001 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaagga
    12061 accgaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    12121 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcac
    12181 gcttcctggc ttgccagcca catggcgtgc acggcatttt catagtaggt catatcgacc
    12241 agcgcactgc cgccatgcgg taacagaata ctgccgtagt gatgcatcat atgcgccaga
    12301 cgagggataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    12361 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    12421 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatc gcgatggtgg
    12481 tgataatcga agtacaggga gggagaagag atatgaataa agttacgcac accccaggcg
    12541 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    12601 gtcccccagg gcgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    12661 actttagctt gtgatgagac caactcggtc agatccgccg gaacaaactc tgcgcccatt
    12721 ttttccagca atttacccat tgcctcgttg cgaccggtcg ctcgcacgct gatgcctttc
    12781 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    12841 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccct
    12901 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    12961 ctgccagtct ggcgattcgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    13021 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagca cggacatcga
    13081 ttgtacgata gtcgccttta tcttccaggg taaaacagag tcgtcccagc cccggcgctt
    13141 tcatgccaaa taacaacgta agttgttttt ccggttcaac gacaatgact ttccagctat
    13201 ccacagcatc gccggtctgt aaatattcgc gctccgggcg gccttttgcc agcttatgac
    13261 cgatcgcaag gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    13321 ctttaccgcc gatttgattg accacccgcc atagcgcctg aaggctggca gacgttttaa
    13381 cggtaaaccc cgcctgtttg gcaaaataac cgtattctgg tcgccagcga gcaaaagcct
    13441 gcgcgtcata tccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    13501 gtaccgcgtc atcgaaagcg atcagcggtt gtggaatgag tgcacgtaac gcggtgtcat
    13561 ccgccagcag atcgtgtttc agcccctgaa tcaacgctct ggcggtggtg ggcggtactg
    13621 aagtaatcac attaagaaac cacaccgaaa tccagcgggt ggggaggggg atggggagca
    13681 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    13741 cttctggccc agcggcttcg aagatgcgat gttcgcaagc cggatgatct aacagcgcca
    13801 caagatagtg cagcaggttt tccagcgcga tgggcgtagt gcgtgaacgt acccagcgtg
    13861 gtggcgttaa tactggcagg ttgtaaacca tatcgcgcat gacttcgaat gccgctgaac
    13921 ccgcgccaac gataattccg gcacgtagtt cggtcaccgg gacacccgct tcacgtaaaa
    13981 tatccgccgt ggcctgacga gcacgcagat gatcagattg ttcatgaggc ggtgcctgca
    14041 atgaactgag aaagatgact tgctttaccg gtacttcgcg cagcgcatcg cggacgttga
    14101 gagccacctg gcgctcctga gcgataaaat caccaccttc gcccatgctg tgcaccagaa
    14161 aatagacagt atcaatattc tgcaacagga ccggaaggtt atccggccag ttgagatcga
    14221 ctttatggca actgacatta gccagttgca gttttgcaag tctgtcgacg tgacgtgccg
    14281 ccgccaggat ctgatgccct tgctggctga gtgtgtgcac cagatgttga ccaatgtagc
    14341 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaac
    14401 gcacgccagt gggcggcaac ttccgccagt tgttcacgcg agacgtcaag atgggtcacc
    14461 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    14521 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tggtgtcctg acgcatcaca
    14581 tccgcgcctg cttcacgcaa ctgctccgcc atccaggcgg cgttatcgtg gtcttcctgc
    14641 aaacgtgcga cgttattttt cagcgcatac atcccggcgg cagccagaat gccggactga
    14701 cgcatcccgc cacctgtcat tttccgccag cgaatggcac gtttaatgta atcacgatta
    14761 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgag
    14821 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaaaatg
    14881 cgcgcaccgt caacgtgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    14941 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    15001 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgta
    15061 ccgtcggcag ctgcgtcgat gggttgcggc tgaatactgc ccagtactgc tgcgccaccg
    15121 gcttcaaaca gatagttatg cgcggcctga ccgacgatat actcttcgcc gcgttcgcag
    15181 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    15241 ttaccggaaa actctgctgc atagtcctgc agagcattaa cggtagggtc gtctccataa
    15301 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    15361 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    15421 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcaccgcg cccgctgatg
    15481 attgcgcgca gcatatacag gctgaaacct ttggcctgtt cgagtttgat ctgcggtgga
    15541 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatggagaag
    15601 gcgcgttgca gggcttcatc gatttcagac gctttttcta cgcggatacc cgtaatgccg
    15661 caggcttcgg caattcgggc aaagttggtg tcgtgtagtt cggtgccgtc agtcaaatag
    15721 ccaccagctt tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    15781 ttcactggca gcttcatttg cactaccgag aggaaatcgc ccatcaacat gctaaaaccg
    15841 ccatcgccgc acatggcgac cacctggcgt tctggctcgg ttgcctgcgc gcccagcgcc
    15901 tgcggcatgg cgttagccat cgaaccatgg ttaaacgaac ctaacaggcg acgcttgccg
    15961 ttcattttta gataacgtgc cgcccacacc gttggcgtac caacgtcaca ggtgaaaata
    16021 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    16081 tcgctcggtt tagctaaatc gtccagccct ttgcgggcgt cgcggtaatc ttccagcgcc
    16141 ttatccagaa acttgcgttc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt
    16201 gatttgatat cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctggg
    16261 ttgatatcaa tctgaataat tttggcatcg gtcgggtaga acacacggta gggaaactga
    16321 gtaccgagca gcactaacgt atcggcgttc atcatggtat ggaaacctga cgaaaagccg
    16381 attaatcccg tcattccaac atcatatgga ttatcgtatt cgacatgttc tttaccgcgc
    16441 agggcatgga ctataggcgc tttaattttc ccggcaaact caactaactc tttatgcgcc
    16501 cccgcacagc cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc
    16561 agtttgcgta actcttcttc ttccggtgtt actaccggct gtggcgcatg ataccagtgg
    16621 gtagttgccc cttctggcgc aggttttaac gccacatcgc ccggtaacac aacaacggaa
    16681 acgccacggt taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctct
    16741 gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt
    16801 tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc
    16861 ggaacgtgat tgcggtggca atcgaacagg ccgttgatta agtgcaggtt gccgggaccg
    16921 cacgatccgg cacagaccgc cagttctccg ctaagttgtg cttcagcgcc agcggcaaat
    16981 gccgccactt cttcatgccg ggtggacatc cactcgatgg tgcccatgcg attaagactg
    17041 tcactaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat
    17101 tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat
    17161 gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg agaaaaagga
    17221 ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcatcagg cgattatgcg
    17281 aaaaccaaat ccccctgcgg atggcatgag caggccagta cgtaaccttc agcgatttcg
    17341 gcgtcggtca gcgtcattgt gctgctcacc gtatattcac cggaaatcac tttcgtctta
    17401 cagcagccgc aaacacccgc acggcaggcg gcgacaaccg gaacgttatt gctttccagc
    17461 gcctccagta gcgtggtgcc aaccggagcg taaaattctc gtgccggttg cagtttggtg
    17521 aatttcagac cgctggtcgc cgcttccgct actggggtga agaatttctc tttaaagaaa
    17581 cgcgtcacgc cgagcgtttt cacttcctgc tctacccaat ccatatatgg agcagggcca
    17641 caggtcatca cggtacgtga ggctaaatca gggacacctg ccagcagttc gcgagtgaga
    17701 cgaccagcga taaagccttc ggtaacgtta ttttccgcca ccagcgttac cggatagtta
    17761 cgccactcat cggcgaaaat cacatcctgc ggcgtacgca cgttgtagat cacctgcaca
    17821 tcggcctgtg gacggttctt cgccagccag cgacgcatcg acataatcgg cgtgacgccg
    17881 cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc
    17941 atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccaccgggag
    18001 ccgacaccgt catcaatccg ccgcacagtc agggtgatgt attcactcac gcctggcgtg
    18061 gaggaaatgg tgtaagcacg cagcgtttct gctgagtttc gcacgctgac cagcgcatat
    18121 tgcccggcgc gatacgggta gtaatcgtgg caaatcaggg aaatcgtcca cacatccggc
    18181 gtttcttgcg taatgtgatg aacctgcatc cgccacgggc attgattcgt tggcatcgtc
    18241 atcgacaaac tccttacgcg ctcaacagtt gcttcatgtc ttcttcaaca gtggtgatag
    18301 aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg
    18361 cagtcgggcc ggtgacgata tttttcacac ccagagaaag cagcgtcagc agaatgacaa
    18421 tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttg actccgcagc
    18481 ccagtttctc tgccagcgtt accgccagaa taattgctga gtaagcatcg ttacactgac
    18541 cagcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagttta ttaaagcgat
    18601 atttaccgca agcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg
    18661 tgaagtagtg acgctcgccg cgtgcgccgt cacagccgcc aagcaggaag atatgacgca
    18721 gtttttcacg gctcaccaga tcaatcagcg tatcggctgc gccaagcagt gtctggcgac
    18781 caaagcccac ggtgatcagg tgcggaattt cgctgtacgg gaagcccgcc atctgttgcg
    18841 cctgggcaat gaccgcagag aaatcttcgc catccagatg gcgcacaccc ggccagccaa
    18901 cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt
    18961 tcgaggtcat cacgatgggg cctgggaaac gagcgaactc cacttgctga ttctgccagc
    19021 cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtaa ccatgcgcag
    19081 gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt
    19141 tgtagagatc tttgagatcg tgaccggaaa tcaggatgca tttacctgcc gtcgctttga
    19201 cattgacctg ggttggggtc gggtgaccgt atttaccggt ttcgcctgcg tccagaatgc
    19261 tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat
    19321 cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt
    19381 cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca
    19441 ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg
    19501 taaattctgc tgcctgacgt tgcagctcgc cgagatcgtc gctcaccagt tgcaggtcag
    19561 ccatcgggtt atcgacgcgg gcgttggcat ctacagccag gcattgtgct ttcagcgcct
    19621 cgcgcagggc aatcgcttca cgagcgtagc cgataatacg cggagaatcg aagttaacgt
    19681 tggtcagggt tgagaaaaag gcacgtggcg caaagctgtc tacatcgtgg ttgatgatgc
    19741 cgtattcacg tgctttcact gcccaggccg aaagcccttg cagcgtcgcg atgagtaaat
    19801 cctgaaggtc agaggtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
    19861 ttcctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
    19921 aagttatatt taatatacat gtttaaggtt aagacgctta acgcggggat aaaagagatt
    19981 tttaatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcagag atctaccgcc
    20041 agagaggtat tatgcagaga aaaaggcgat gagaatcggc actaacagac taagaataaa
    20101 accgtgaaca atcgccgccg ggaccatatc cagcccgcct gtacgttgaa gaacgggcag
    20161 ggtgaaatcc atcgacgtcg caccgcataa gcccagtgca gtagagcggc tacggcgaat
    20221 cagcccaggg attaacataa tagcaatcag ctcacgggcc agatcattaa aaaacgccgc
    20281 gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc
    20341 gaaaccggag gccattgcca gcgcggtatt gatgggaaga tcaagaataa aggcgttaat
    20401 taaaccacca attaatgaac tggcaaccac caccacggcg acaatcattc cccggcgatt
    20461 aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccga cgaggaaaag
    20521 cagcagaatt aaggtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact
    20581 cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat
    20641 cgcaatacgc gacgggagtt tttcttgctg atggtggttg ctccatggca ggccgcgctc
    20701 cagccacatc agggcggcaa tattacacag taaaataacg gtagtactga cggcagaata
    20761 atgcagaatt gccaacaagt tactggcgag gttatcgaga aacgccagac tgatacccat
    20821 aaaaaagaga ataaggtaaa ccatccagct taagagctga ttaataactc ttaatgcagc
    20881 tttttggcga agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc
    20941 agaaaacatg aaaacccagt ccttgcaaag aagattcgaa atgcgcgatg acacactact
    21001 gaaagcggaa ggacgagtaa agttgcaatt aaaaggaaat gttatgcata aggatcggta
    21061 gggtattcat tttcatttaa agatattctt gcgctttaat tacaaaccgc accggtgttg
    21121 gtggcgtcaa aatcgccgac ccggtagccg taagggataa gtcgagggca c
//
LOCUS       DAOFSV010000073        20535 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.073, whole genome shotgun sequence.
ACCESSION   DAOFSV010000073 DAOFSV010000000
VERSION     DAOFSV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.073"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(179..1522)
                     /gene="gdhA"
                     /locus_tag="QBH27_002811"
     CDS             complement(179..1522)
                     /gene="gdhA"
                     /locus_tag="QBH27_002811"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDQ7015105.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            1758..2030
                     /locus_tag="QBH27_002812"
     CDS             1758..2030
                     /locus_tag="QBH27_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDQ7015106.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(1996..2403)
                     /gene="nudG"
                     /locus_tag="QBH27_002813"
     CDS             complement(1996..2403)
                     /gene="nudG"
                     /locus_tag="QBH27_002813"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDQ7015107.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKIEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            2490..3110
                     /locus_tag="QBH27_002814"
     CDS             2490..3110
                     /locus_tag="QBH27_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDQ7015108.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3119..4426)
                     /gene="ynjE"
                     /locus_tag="QBH27_002815"
     CDS             complement(3119..4426)
                     /gene="ynjE"
                     /locus_tag="QBH27_002815"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDQ7015109.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4493..5146)
                     /locus_tag="QBH27_002816"
     CDS             complement(4493..5146)
                     /locus_tag="QBH27_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ7015110.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(5146..6681)
                     /locus_tag="QBH27_002817"
     CDS             complement(5146..6681)
                     /locus_tag="QBH27_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7015111.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPSNTLASFLPLTGVLCVVLLAILADQSTINSQALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVVKWVGYVRQ
                     GLR"
     gene            complement(6654..7820)
                     /locus_tag="QBH27_002818"
     CDS             complement(6654..7820)
                     /locus_tag="QBH27_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ7015112.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTLQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(7830..8378)
                     /locus_tag="QBH27_002819"
     CDS             complement(7830..8378)
                     /locus_tag="QBH27_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDQ7015113.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8378..9085)
                     /locus_tag="QBH27_002820"
     CDS             complement(8378..9085)
                     /locus_tag="QBH27_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDQ7015114.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(9099..9776)
                     /gene="ydjY"
                     /locus_tag="QBH27_002821"
     CDS             complement(9099..9776)
                     /gene="ydjY"
                     /locus_tag="QBH27_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDQ7015115.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(9781..10491)
                     /locus_tag="QBH27_002822"
     CDS             complement(9781..10491)
                     /locus_tag="QBH27_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDQ7015116.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(10658..11464)
                     /gene="xthA"
                     /locus_tag="QBH27_002823"
     CDS             complement(10658..11464)
                     /gene="xthA"
                     /locus_tag="QBH27_002823"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDQ7015117.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     ITVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11910..13130
                     /gene="astC"
                     /locus_tag="QBH27_002824"
     CDS             11910..13130
                     /gene="astC"
                     /locus_tag="QBH27_002824"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDQ7015118.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWEQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLER
                     FAAACEHFVSRGSS"
     gene            13127..14161
                     /gene="astA"
                     /locus_tag="QBH27_002825"
     CDS             13127..14161
                     /gene="astA"
                     /locus_tag="QBH27_002825"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDQ7015119.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSESGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            14158..15636
                     /gene="astD"
                     /locus_tag="QBH27_002826"
     CDS             14158..15636
                     /gene="astD"
                     /locus_tag="QBH27_002826"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDQ7015120.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFSDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLLPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            15633..16976
                     /gene="astB"
                     /locus_tag="QBH27_002827"
     CDS             15633..16976
                     /gene="astB"
                     /locus_tag="QBH27_002827"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDQ7015121.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            16969..17937
                     /gene="astE"
                     /locus_tag="QBH27_002828"
     CDS             16969..17937
                     /gene="astE"
                     /locus_tag="QBH27_002828"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDQ7015122.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            18267..18752
                     /gene="spy"
                     /locus_tag="QBH27_002829"
     CDS             18267..18752
                     /gene="spy"
                     /locus_tag="QBH27_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDQ7015123.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            18955..19530
                     /gene="ves"
                     /locus_tag="QBH27_002830"
     CDS             18955..19530
                     /gene="ves"
                     /locus_tag="QBH27_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDQ7015124.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(19490..20377)
                     /gene="cho"
                     /locus_tag="QBH27_002831"
     CDS             complement(19490..20377)
                     /gene="cho"
                     /locus_tag="QBH27_002831"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDQ7015125.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRASNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQALERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
BASE COUNT         4896 a         5298 c         5512 g         4829 t
ORIGIN      
        1 gctcccggcg gtggagaagg gaagaaaaag gattcgccac gcaagagagc gccgaaaaaa
       61 agcccgccat cagaagaggc gggcaatgga gtgataatat aaaggttctt tcaaccatct
      121 gatactgaag atttttatgg ccggataagg cgtttgcgct gtatccggca caattgtctt
      181 aaatcacgcc ctggcttaac atcgcatccg caaccttcac aaaaccggca atgttcgcgc
      241 cctgcacgta gttggtttgc tcaccttcac caccatgctc aacacaggca tggtggatat
      301 ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc tttccagccc aggcgcgcag
      361 cgttttgcgc catttccagg cccgatgttg ctacaccgcc agcattagcc gctttacccg
      421 gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc ttcgatggtg gtcggcatat
      481 ttgccccttc ggcgacggct ttaacgccat tggcgataag ctgatgcgcg gcgtcaacat
      541 ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac cggtacagac cacggttgtt
      601 ggccttcgag atagaccaga ccaaattctt tggcgtaatc tgccactcga ccatcgcggc
      661 tggctttgat ttcgataagg cgcgccagtt tctctttcgt gaatccgctt tcatcaacta
      721 cagtgccgct ggagtctgac gcagtgatca ctcgagcacc aaattccatc gctttttcga
      781 tagcgtactg ggcgacgttg ccggagccag aaacggaaac gcgcatccct tcaaaaccca
      841 taccgtggcg ttttagcatt gcttctgtga aataaaccag accgtagccg gtagcttccg
      901 ggcgaataag actgccgcca aatgaaaggc ccttaccggt gaagacgcag gcggtattgt
      961 tggagagctt tttcatcatc cccgccataa agccgacttc acgaccacca acgccgatat
     1021 cacctgccgg aacgtcagta tccgcgccca ggtggcgata cagttcagtc atcagcgcct
     1081 ggcagaaacg cataacttcg ccttcgcttt tccctttcgg atcaaagtcg ctgccgcctt
     1141 taccaccacc catcggcaga gtagtcaggg catttttaaa ggtttgctcg aagccgagga
     1201 atttgagaat ggaaaggttt actgaagggt ggaagcgcat accgcctttg tacggaccga
     1261 tggcggagct gaattgcaca cgccatgcac ggttgacctg cacctggttg cgatcatcaa
     1321 cccatactac gcgaaattgg atcacgcgct ccggttcaac cagacgctcc agtaatgaca
     1381 tctgacgata tttgggattt tgttcaagaa aaggccagag tgtggtcatt acttcacgaa
     1441 cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg gacatggttg aggaatgact
     1501 ccagagaata tgtctgatcc atagatataa aacccttata tattaatacg attatgctta
     1561 tgttgtttat gtcatgtgct tttgcagttt tcgactatac catcaagaat gtgacggtaa
     1621 gcaagaaaaa aatgacccag gaaagcaaat taataacgag agtaatctca taaccaaatg
     1681 cgataactca gacgctaaag ttttgccgcc cggctaccgt tatctgtaga agagacaata
     1741 acgttaagga gttccctgtg agtcgagcat tgatcgccgt agtattagca tttccattaa
     1801 ttacactggc taacccgcat tatcatccgg acgtggaggt aaacgtaccg ccagaagtgt
     1861 tcagttcagg cgggcagtca gcgcagccct gtactcagtg ctgcgtttat caggatcaaa
     1921 attattctga aggggcagta attaaagccg aaggaatact gctgcaatgc caacgggatg
     1981 acaaaaccct aagcactaat ccgctggtct ggcggcgcgt gaagccataa acgcctctaa
     2041 taatggaatg tcagcagggg ccagcggata tcgcagcgcc tcttcaggtg agcaccagac
     2101 cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag tcgggtacgt gccaggcatg
     2161 aagatggata atccgccccg aaacttctcg ctgatggctg gcaacatagt caccaacagt
     2221 tgcttcgatg cccagttctt cgtttaactc acgcaccagc gcctgccgct ggctttcatc
     2281 cggctcgatt ttaccaccgg caaactccca taatcccgcc tgatcgctct gggcagggcg
     2341 ttgcgcgagt aaaattttgc catcacgttc gatgatggcg gcaacaactt caatcatttt
     2401 cataaattac acacgggcag ataagcgaaa aatgccataa tatccgcact gttgctcaat
     2461 cagaaaggct ttatatttaa ggagtgcctg tgctagaccg ccatcttcat ccccggatta
     2521 aaccgttgtt gcatcagtgc gtgcgggttc ttgataaacc gggcattacg ccagatggtt
     2581 taacattagt gggatttgcc atcggcgtgc tggcactgcc ttttctggcg ctgggctggt
     2641 atctggcggc actagtcgcc attttgttga acaggctgct tgatggtctg gacggtgcgc
     2701 tggcgcggcg cagagggctt accgacgcgg gcggctttct cgatatttct ctcgattttc
     2761 ttttttacgc gctggtgcct tttgggttta tcctcgccgc accggagcaa aacgccctgg
     2821 caggcggctg gctgctgttt gcgtttatcg gcacgggcag cagttttctc gcttttgctg
     2881 cgctggcggc gaaacatcag attgataatc ctggttatgc gcataaatcg ttttattatc
     2941 tgggcggctt aaccgaaggc agcgagacga ttttgctgtt tgttctgagt tgcctctttc
     3001 cggcctgttt tccgtggttt gcgtgggtat ttggtgcgtt gtgctggatg acgaccttta
     3061 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
     3121 atttactgct gtccgggccg cgttcaccgg ttgctaccgg attttttgga tcgctgctcc
     3181 attcgtacca gccaccgtcg taaacggaga cgttattcca gcccattgcg cgtgcgtaca
     3241 taaaggtttc tgacgcgcgc cagccagtgc cgcagtagaa tgaaacttgc tgatctggtt
     3301 tgatattcca tgctttccac atagcggtta tatcatcggc gctgcgcatg gtgccatccg
     3361 ggttatggaa atcttccata tgcgtcgagt cgctacctgc gtgtccccaa cgtgctccgg
     3421 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
     3481 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgc gcttgttcca
     3541 tatcaagcat cagttgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca
     3601 ctttcggtgg cgtaccgcgc tcaacgggca gacccgcgtc ggaccaggtt tgccagcctc
     3661 cgtccagcag gcgcacatct ttcacgccgg cataaagcat aatttgcgcc acacgcgctg
     3721 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg
     3781 ccagcatcgc tttcagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
     3841 cgttggtgtc gatgtagtca gcgccgggaa tatggctgat aaggtaaaat tttggagcgc
     3901 cccaggccgc ttcaatgact ttccagtcac cggcaggttt cgccgtaacc tctttacctt
     3961 gttgcaggtc atgcagccac tgcggatata ccagctgttc aaagtgcggc agtttttgca
     4021 gacgggaagg ttcgcttagc gcgtcactca ggatggagat atgcgtaaaa cctgcttttt
     4081 gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc gtacagtgcc accggggcat
     4141 cggctttcag gttatgttgc ttgatccact cgttgagctg ttcggtgctc attttgtcga
     4201 gccagctagc agagaggttt aaggcggcag gttcatgacc ggaagggcca tttaaggttt
     4261 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgtt
     4321 gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
     4381 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
     4441 ttgtagtttt gagagcgcca ttctgaactg acgcatcggg aaaaactttg cgttatcgca
     4501 gtttgttgta attttctgac cactgcgcca tatccagaac ggggctatca ggaggaacat
     4561 cctgaagatc gtgcgtcacc tgcacaacag ggatcgccat ggcgcgaatt tcgctgaaca
     4621 cccaatggcg aaaattatcg cgcagggcca catcaagacg gctgaatggc tcatccagta
     4681 gcaacgcttt tggctgagcg agaagggcgc gaagcagagc aacgcgcgct cgctgaccgc
     4741 cagacaaagt ggcaggatcc tgatggaacg ctccgtcaag gcctgaacgc tcaagtgcat
     4801 cattcacggc attacgtcgg gcattcccct taagtgtcgc cggtagcgcc agcagtaaat
     4861 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg gaaaagaatg ccaatctgac
     4921 gctgtgcggt gggtagcatg tcaatccgtt gctcattaag ccatagctcc cctgtacaag
     4981 aaaactgtcc ggccagcgca ccaatcatcc acgaaaacag agtggatttc ccgcagccgg
     5041 atggtcccat taacgtgaca atgtcacctt tatctactgt aaagttaacg tttgtcagca
     5101 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggagtccttg
     5161 tctgacataa cctacccatt ttacgactaa cgcggtcagg gcaaaaataa taagcggtaa
     5221 cagcagttgc cataaagcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
     5281 caccgcttca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
     5341 ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcgcagggc gcaacatgag
     5401 tgggcatttt acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
     5461 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
     5521 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatccaggt tcagccatag
     5581 cgccagcgtg tactggcccg ccaccagcgg cagagcgggt aataaaatgg gcaaccatag
     5641 ccacaactgg cgacgctgtg gcccccattc cagccacagc agtaacaaga gcaaagcgat
     5701 gaatgccgcc accagcccca ttgtcaggct gttgatgagc gcttgactat tgatcgtcga
     5761 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
     5821 cgccagcgta ttgcttggca ataaaggcgt ggcaggctta cgaacgccat ctacgcgggg
     5881 aatagtacgc cgccagccac gccatagcag atagcccagc aaaacgtagg cggcgagtaa
     5941 cagcatcagc agcaggctgg cgagtgcgcc tttcgtttgt tgatcggcgt cgccctgagt
     6001 taaccactgc cagctaatta ccgccagcgt cgggggatta cctggcccaa gaataatcgc
     6061 cacatccacg accgacagcg accaggcgac aatcgccagc atcgccattg ctaacgcaga
     6121 cgcgacggag ggcaacagca gccagttcag gcattgccag cggctgtagc ccagcgaatc
     6181 cagcacaatg agttgctgca acagccgttt ttcgctcaat actgccgcta agatccacag
     6241 cagaaatgcg ctttctttca ccgccagggt gaggcccagc ccaataccca atttgtccat
     6301 tggcggagtg aaatacggga aatagtcata aagcagccct ccgtcagcaa agagcagaag
     6361 ggcgctggtg gcaaaagcca catggggaat ggcgagcagc cacggcagac gggcgcacat
     6421 acgctgccat ttcggccctg gccacaacgc cacaatcacc agcagggcaa tcaacaacgc
     6481 cccgaccgcc gcaatggttg tcgacaccag cgtcgccagt aatgcctgcg gtaactgtgg
     6541 atcggcaaat aacgcctgcc agtgcgtcaa cgacaaagca ggcgatatca gcgtgagagc
     6601 tgccgggatc agcggtgcat aaatcaccgc tatcatcgcc cacagcaaaa agattaatgc
     6661 gtaccgtaac ggtgtagcca ttcttgttcc agcgcattta cccaacctgc gtgcggttca
     6721 gccagtaccg gcggcagatc ctgcggcatt ctcgattgca atgtttcgcg ctgcccgtca
     6781 ggcagttttt gcgggtcgag aacagaagga tcgccccaga cagcaggatc tgctttacgc
     6841 agttgcgcat cgggtgagag caggaaattg gcaactacct tcgccgcagc actggcattc
     6901 gcgttggcag gaatggtgac gaaatggacg ttgccaatca tcccctcgcg aaaaccaaaa
     6961 ctgtaactgc ttgcaggcaa atcgccgctg gcgatttttt gctgcgcatg cgcggggtta
     7021 aaggtcagcg acaggcgcaa tgtgttagct ttcagcagag catccatccg cgcgggcgaa
     7081 ggcgggaaat cctttccttc gcgccacaaa tacggatgca gtgcatcaag atattgccac
     7141 aagggcgcag tgacgcgggc gaaagtggca tcgtccggcg cttcttttaa tgctgtggga
     7201 tctggcgtta gcataatcag caactgttca agaaacgccg tgccggtaaa gtccggtggg
     7261 cgcggatagg taaccgtgcc gggattggct ttagcaaact ccagtaaggc ttgcggcgtt
     7321 tgtggtggct gtagcgtaac atcgcggcgg gcgataaacg tcagttgtgc gccgccccag
     7381 ggcgattcag ccccttctgt aggcactgaa aaatcttccc gcaccggaag ctgtgtgtcg
     7441 acatagcgcc agttgggcag agtctccgcc cagccggttt gtaataaatt ggcctctttt
     7501 aaggtgcgga agttttcgcc gttcacccag agcagatcca ccgaaccgcc cgttttacgt
     7561 ccggcagcaa cttcggtctg aatgcgtttc accgcgtctg cggcatccgc gagacgaaca
     7621 atcttcaggt ttatagcgta atgggttttc atctcgccgc taacccagtc gagatagcgg
     7681 ttaattgcgg tatcgccgcc ccaggcgtta aaccagacgg tttgtccttt ggcctcattt
     7741 tttatttcct gccagtcact ggcatgtgtt gccaaagaaa acagcgataa caatcccagc
     7801 aaccacccac aatggcgcat cttctctcct taagaacgtt tttcaggaat acggcacagc
     7861 ccctgagcgg gaatgtccat ggcagaatta aaacgggctg acagattttg caggcctatc
     7921 aatgccgtca gttcggtgag cgcctgagcg tcaaaatgcg tagccagtcg ggcgcgcaga
     7981 gcatcatcga ccgttggcgg cgttacgctt gcggcttcgg cgtactccag cgccagccgt
     8041 tcttcatcgc taaagagcgg gctttgccgc cagtcagcca ctgccagcag tttatcggtg
     8101 ctgccggtac gctcggcgac tttcatactg gtgatatcca cgcaaaactc acacaggcac
     8161 atttgcgcaa tgcgcgcgct gacaagcgat cgtactaccg gatcgagcgg tgagcgtttg
     8221 cgctccagcc agccgacaaa catcgacacc agataaaaga taaacgggat ccgcccccac
     8281 cagcgaatcg ggcttagcac ctcgccgtaa tggcgacgat gcagccacgc ctgcggacgt
     8341 aaaatgaaag gaattttgct aagcggcggt aaacccatca ggcattcctc ttctggcgct
     8401 caagccatat tttcttcgcc ataaaaatca ccacggtcag ggcaaacaga ataaacagtc
     8461 cggtgacaaa ccagaaagta ccgcctgtta acatgctgcc cgcccaggaa taaacaatag
     8521 tcgccggtaa ttgaccaagc ccggtggcga taaaaaacga gcgaaaacgt attgaagtca
     8581 aaccggcagc atagctgatt ggatcgaaag ggacaaaagg caataaccga cataccagga
     8641 tggtgtgttt gccgtagcga gtgaaaaagc cgtccatact gtcaagtacg gttttgccgg
     8701 ttaatttttc caccacttcg cggcccatca ctctggcgat aaaaaagcac agcgccgcgc
     8761 cggccatcga actggtccac gacagtaagc cgccccagaa cgcgccaaac agcgacgcat
     8821 tggcaaaggt gatcaaaaac gcaggtagcg gtgcagcaat ggcctgtaaa atcatcaaca
     8881 agaacgagac aactgccgct agtgcgccgt aagactgaat aaagcgttct ataccctgtt
     8941 ggtccacggc ggcaaacgcc gcaacgctgc gattgataaa ctcatggacg ccgggaagca
     9001 gcgcccatgc cagcagcaca gcgaacaaca ggatgatgag ggttatacgg tagtaccaga
     9061 ttttacgcga ttgcatcttc atcatccttt atcctgcttt attcggtgat tttaaaggta
     9121 accgttgcca gcgtgttatc cgccgggaga actgaggcat tgcctttgaa tttcacttcg
     9181 ccgcgttttt ccaccgcacc ataagtgtat gttgcattgc tgacgatgcc gaccgggcag
     9241 ctatccagac acaccaggca acccgttttc ttctcttctg ctgccgttaa attaccgcca
     9301 aagcgcatgt ccagtttctt gccattactg tcaacaatca cttcatcgaa ggaatacgct
     9361 tttgccgccc cttgccagtt gaccgaaata tccagtttgc tgcctgtgac atgagtcgtt
     9421 tctttattat ccattgtcat gttttcgccc ggcgtaccac ctgcctcttt cagggcttca
     9481 taaaatgctt tcggggtcgc ataacccata aacagcgatt tatgtccgtt ggagccatct
     9541 ttaaacacaa taccgtgacg agtgtcgtcg gtgaggaaac gaccattaat ttgcaccagc
     9601 atggttaccg tatggttatt ggcatcgaca cgcagcggtt ggctgttcga aagtccgtcg
     9661 tattccactt ttgcagccgc attctctttt tgatcgcagc cgctaagccc agcaacagcc
     9721 agtaagcaga gtgcagccag tccttttttc catgacactg aataatgttg caacatcgtt
     9781 ctatccttca ttttttgggt gagaaagtgg gctgcggcga gaagcagaca gatccacatg
     9841 tttgtgtcgg gcgtagagtt ttgcgagctg gacgagaata aacagtgcca ggcccgccag
     9901 acagagttgt aaaataaagc gcagcgtaat gccttcattg gcgagatcgc ttgccatcac
     9961 ggtataaata acaataccgg gtagggtcgt gagtgccgaa ataagggtat aaggccagaa
    10021 ggcgattgtg gttaatccgt aagcgtaatt ttgaatattg taaggaaaca acgggattaa
    10081 gcgggtcaga ataagaaaat caataccgtt acgcgcaatg cctttttcaa tggcctgaaa
    10141 ggtatggcta tggccaacgt atttcagcag taaatcacgt cccagccagc gcgccagaag
    10201 gaatgagcac gaggaggcca gcgtggcggc aattaatgat agtagtgtcc ctaagagcgg
    10261 gccaaaaact attcctcccg cgatcaccag tatgcttcct ggtaacagaa agagggtggc
    10321 aatgatgaat aacagaatat agaggctata gccgaacaat ccgctctggc gaatgagtgt
    10381 ctgtaagtgg ggtaaatcgg tgagcagatc gaataaaccg aaagcgtgga tagcgtaaat
    10441 gaccagcgca aaaataagac aggcaaaaag aaatttacgc tcagcgttca tcgtggagtt
    10501 tcctatatca aacttgataa tagagaagtg gtcagacaac taaaaacgca gtgacggtat
    10561 aacaaaggac ggcgggcaac aaatcattac tgacagtttg ttgatttttc tcaaggttaa
    10621 ttctcctgac ccagtttgag ccaggagatc agctaaatta gcggcggaag gtcgcccaga
    10681 cgggggcgtg atcggacggt ttttccatgc tgcgaatttc atagtcgatg ccggtttcta
    10741 cgcaacattc tgccagcggt tggctggcga gcagcaggtc gatgcgcaga ccgcgattat
    10801 cgtcaaaacc ttttgagcgg taatcaaacc atgagaaacg atctgctgtt tgcggattcg
    10861 catggcggaa ggtatcgacc aacccccagc tcatcagcct ctccatccat tcgcgttcct
    10921 ccggaaggaa agaacatttt ccggtacgca gccagcgctt acggttctct tcgccaatgc
    10981 caatatccag gtcgccagga ctgatattca tatcgcccat aatcagcacc ggattttcac
    11041 gcttgagttc ggtttccagg tagttttgca gattctgata aaactgcgcc tttgccggga
    11101 attttatcgg atggtcgcgg ctttcaccct gcgggaagta accgttgatc acggtgatat
    11161 tgcccagcgg cgaggggatt tccgccataa taatccgccg ttgcgcctct tcgtcgtcac
    11221 cgggaaagcc gcgacgcacg gaaatcggcg tctctttggt cagcagcgcc acgccataat
    11281 ggcctttctg cccgtgataa aacacgttgt agccgagctt cgccacctct tcgagcggaa
    11341 acatatcgtc atgaactttt gtctcctgca ggccaatcac atccggttgg tgcttttcga
    11401 cgatggcttc aagctggtga ggtctggcgc gcaggccgtt gatattaaaa gagacaaatt
    11461 tcatagtcgc tgccatttat tcagataaag agtgcgtgga tggtagcaga atttcgcttt
    11521 gcttaccgcc tgttgttaag tgatttgatc gatggttgtt aatgatgtca acgatggcgc
    11581 aaaaaatgcc cgcttttggt gcgcgttgcg tcagaatggc gcagtaattt ccagtaaatt
    11641 tcgtcaatga tcacaattcc agaattatat tttacttctg cataacattg cgttttttat
    11701 tctttttatt cacccacatc gcaaacgtat tcactttata tgcactttaa atgcatatga
    11761 tgtggttata actgcttgat ttccagttag cctccgccgt ttatgcactt ttatcactgg
    11821 ctggcacgaa ccctgcaatc tacatttaca gcgcaaacat tacttattat taacatataa
    11881 ataactaatt atttactgta gaggtcgcta tgtctcagcc aattacgcgt gaaaactttg
    11941 atgaatggat gatacctgtt tacgctccgg caccgtttat accggtacgt ggcgaaggtt
    12001 cgcgcttgtg ggagcagcag gggaaagagt atatcgactt cgcgggtggc attgcggtga
    12061 acgcgctggg ccatgcgcat ccggaactgc gtgaggcgct gaacgaacag gcgagtaagt
    12121 tctggcatac cggcaacggt tacaccaacg agccggtact gcgactggcg aaaaaattga
    12181 tcgacgccac gtttgccgat cgcgtcttct tttgtaactc cggtgcggaa gccaacgaag
    12241 cggcgctaaa actggcgcgt aaattcgctc acgaccgcta cggcagccat aagagcggca
    12301 tcgtggcgtt caaaaatgcg tttcatggtc gcacgctgtt tactgtcagt gcgggtgggc
    12361 agccagccta ttcacaggat tttgcgccac tgccgccgga tattcgtcat gctgcatata
    12421 acgatattaa ctctgccagt gcgctgattg acgacgccac ctgtgcggtg attgtcgaac
    12481 ccatccaggg ggaaggcggt gtggtgccag ccagcaacgc atttttacaa ggtctgcgtg
    12541 aattgtgtga ccgccacaat gcgttgctta tctttgatga agtacaaacc ggtgtcgggc
    12601 gcaccgggga actgtatgcc tatatgcact acggcgtgac gcctgatctg ttaactaccg
    12661 ccaaagcgct gggcggcggt ttcccggttg gtgcgttgtt ggcaaccgaa gagtgcgcca
    12721 gcgtgatgac cgttggcact cacggcacca cctatggcgg caatccgctg gcctcggcgg
    12781 tggcaggcaa agtgctggat ctcatcaaca cgccagagat gcttaacggg gttaaacagc
    12841 gtcacgactg gtttgttgag cgtctaaatt ccattaatca ccactacagt ttgttcagtg
    12901 aagttcgcgg cttaggtctt ttgattggct gtgtactgaa tgccgattac gccgggcaag
    12961 cgaaacagct ctctcaggaa gcggcgaaag caggcgtgat ggtgctgatt gcgggtggca
    13021 acgtggtgcg ttttgcgcca gcgctcaatg tcagcgaaga agaggtgacg accggactgg
    13081 agcgctttgc agctgcttgc gaacactttg ttagccgagg ttcatcatga tggtcattcg
    13141 tcccgttgag cgctcagatg tctcggcgct gatgcagctt gccagcaaaa cgggcggcgg
    13201 cctgacgtcg cttcccgcca atgaagccac gctttcggtg cgtattgaaa gggcaatcaa
    13261 aacctggcaa ggcgaactgc ccaaaagtga gcagggttat gtgtttgtac tggaagatag
    13321 cgagtcaggc actgtggcgg ggatttgcgc cattgaggtg gcggttgggc tgaacgatcc
    13381 ctggtacaac tatcgcgtcg gcacgctggt tcacgcttcg aaagagctga atgtctataa
    13441 cgcattgccg acgctgtttc tcagtaacga tcacaccggc agcagcgagc tgtgcacgct
    13501 gtttctcgac ccggagtggc gcaaagaggg caacggctat ttgctgtcga aatcgcgctt
    13561 tatgtttatg gcggcttttc gcgacaagtt taatgacaaa gtggtcgccg aaatgcgcgg
    13621 ggtgattgac gaacacggct attcaccgtt ctggcaaagc ctcggtaaac gcttcttttc
    13681 gatggatttt agccgcgctg attttctctg cggcaccggg caaaaggcat ttattgcaga
    13741 actgatgccg aaacatccga tctatacgca ctttttatcc caggaagcac aggacgtcat
    13801 cggtcaggta catccgcaaa ccgcacctgc ccgtgcggtg ctggagaaag aaggttttcg
    13861 ctaccgtaac tatatcgaca tctttgacgg cgggccgaca cttgagtgtg atatcgaccg
    13921 cgtgcgcgcc atccgtaaaa gtcggctggt ggaagtggcc gaagggcagc ctgcgcaggg
    13981 cgattttccg gcctgcctgg tcgccaatga aaattatcac catttccgcg tggtgctggt
    14041 gcgtaccgat ccagcgaccg agcgtttgat tttaaccgcc gcacaactgg atgccctcaa
    14101 atgccacgca ggggatcgcg ttcgtctggt gcgcctgtgc gcagaggaga aaacagcatg
    14161 actttatgga ttaacggtga ctgggtaacg ggccagggcg cattgcgtgt gaagcgtaat
    14221 ccggtatcgg gcgaggtgtt atggcagggc aatgatgccg atgctgctca ggtcgagcag
    14281 gcttgtcggg ctgcccgagc ggcgtttccg cgctgggcga ggctctcatt tagcgatcgt
    14341 caggtaagag ttgaacgctt tgccggattg ctggaaagca ataaagctga attaaccgcg
    14401 attattgcca gagaaaccgg caagccgcgc tgggaagcgg caaccgaagt gacggcgatg
    14461 atcaataaaa tcgcgatatc aattaaggcg tatcacgttc gtaccggtga gcagcgtagc
    14521 gaaatgccgg acggcgcggc gagcttgcgc catcgtccgc acggcgtact ggcagtgttt
    14581 ggaccgtata atttccctgg tcatctgccg aacggacata ttgttccggc attgctggca
    14641 ggtaacacca ttatttttaa acccagcgaa ctgacaccgt ggagtggcga agcggtaatg
    14701 cgtttatggc agcaggctgg cttgctgccg ggcgtgctga acctggtgca gggcgggcgt
    14761 gaaacgggtc aggcgctgag tgcgctggag gatctcgacg gtttgctgtt taccggcagc
    14821 gccaataccg gctaccagct gcatcgccag ctttccggtc agccggagaa aattctcgcc
    14881 cttgagatgg gcggtaataa cccgctaatt atcgatgagg tggcggatat cgatgccgct
    14941 gtccatctga ccattcagtc ggcgtttgtc actgccgggc aacgctgcac ctgcgcccgc
    15001 cgtttattgc tgaaaagtgg aacgcagggc gatgcatttc ttgctcgcct ggttgccgtc
    15061 agccagcggt taacgccggg cacttgggat gacgaaccgc agccgtttat tggcgggctg
    15121 atttctgagc aggccgcaca gcaggtggtt actgcctggc aggaactgga ggcgatgggc
    15181 ggacggcccc tgcttgcgcc gcgcttatta caagcaggga catcgttgct gacgccgggg
    15241 atcattgaaa tgacaggcgt tactggcgtg ccggatgaag aagtgtttgg gccgttattg
    15301 cgcgtctggc gttatgacaa tttcgatgaa gcgattcgaa tggcgaataa cactcgcttc
    15361 ggcctctctt gcggtctggt ttcccccgag cgggaaaaat tcgatcaact gttgctggag
    15421 gcacgggcgg ggattgttaa ctggaacaaa ccgcttaccg gtgctgccag taccgcgcca
    15481 ttcggcggca ttggcgcttc cggcaatcat cgccccagcg cctggtatgc cgcagattat
    15541 tgtgcctggc cgatggcaag tctggagtcg gactcgttaa cgttgcccgc aacgcttaac
    15601 cccgggctgg atttttccga tgaggtggtg cgatgaacgc ctgggaagtc aatttcgacg
    15661 ggctggtagg gctgacgcac cattacgcgg gcctgtcgtt tggtaatgaa gcctctaccc
    15721 gtcaccgttt tcaggtgtct aacccgcgac tggcggcaaa acagggctta ctgaaaatga
    15781 aaaaacttgc cgatgcggga ttcccccagg ccgtgatccc gccgcacgag cgtccgttta
    15841 ttccggtgct gcgtcagttg ggattcagtg gtagcgatga gcaggtactg gaaaaagttg
    15901 cacgccaggc accgcactgg ctttccagcg tcagttccgc ttcgccaatg tgggtagcca
    15961 atgcggcaac gatcgcgcca tccgccgata cgctggatgg caaagtgcat ctcactgttg
    16021 ctaacctgaa caataaattt caccgttcgc tggaagcccc cgtcaccgaa tcgctgttaa
    16081 aagcgatttt taacgacgaa aagaaattta gcgtccattc ggcgttgccg caggtagcct
    16141 tgctcggtga tgagggggcg gcaaaccaca atcgtctcgg cggtcattac ggtgaaccgg
    16201 gtatgcaact ttttgtctac gggcgagaag agggcaatga tacccggcct tcccgttatc
    16261 cggcgcggca gacccgtgaa gccagcgagg cggtggcaag gttgaatcag gtgaatcccc
    16321 aacaggtgat tttcgcccag caaaacccgg acgttatcga ccagggcgtt tttcataatg
    16381 acgtgattgc cgtgagtaac cgccaggtgc tgttttgcca tcaacaggcg ttcgctcgcc
    16441 agtcacagtt actggcaaat ctgcgtgcac gggtcagtgg ttttatggca atagaggtgc
    16501 ccacggcgca ggtttccgtg tcagatgcgg tgtctactta tctgtttaac agccaactgc
    16561 tgagccgcga tgatggttcc atgatgttgg tgctgcctca ggaatgtcgg gaacatgctg
    16621 gagtatggtg ttatctcaat gaattgcttg ctgccgataa cccaataagc tcactaaaag
    16681 tttttgattt acgtgaaagc atggcgaatg gcggtggccc ggcgtgcctg cggctgcgcg
    16741 tggtgttgac agaagaagaa cgccgggcgg tgaatccggc ggtgatgatg aacgatacgc
    16801 tgtttaatgc gctcaatgac tgggtggatc gttactaccg cgatcgtctt actgcggcgg
    16861 atcttgccga tccacaattg ctgcgcgaag gacgggaagc gctggataca ctgactcaat
    16921 tattggatct tggttcggtt tatccattcc agcgcgaggg agggggaaat ggataatttt
    16981 cttgctctga ccttaacggg taaaaaaccg gttatcaccg agcgagaaat caacggcgtt
    17041 cgctggcgct ggctgggcga tggcgtgctg gaactgacgc cattaacgcc accgcaaggc
    17101 gcactggtga tttcagcggg aatacacggt aatgagacgg cacctgtgga aatgctggat
    17161 gcgttgcttg gcgcgatatc tcacggcgag atcccgttac gttggcggtt gctggtgatc
    17221 ctcgggaatc ctcctgcgct gaagcaaggg aaacgttatt gccatagcga tatgaaccga
    17281 atgtttggcg gtcgttggca gctatttgct gaaagcgggg aaacctgtcg ggcgcgcgaa
    17341 ctggaacagt gcctggaaga tttttatgac cagggcaaag aatctgtgcg ctggcacctt
    17401 gatctccata ccgcaattcg tggctccttg catccgcggt tcggtgtatt accgcaacgc
    17461 gacattccct gggacgagaa atttctgacg tggctgggtg cggcgggtct ggaggcactg
    17521 gtgttccatc aggagcctgg tggtacgttt acccatttca gcgccagaca ttttggcgcg
    17581 ctggcctgta cgctggaact cggcaaagcg ttgccctttg gacaaaacga tcttcgccag
    17641 tttgcagtaa ctgccagcgc aattgctgcg ctgctatctg gtgagagtgt cggtatcgtg
    17701 agaacaccgc cgctccgtta tcgggtggtt tcgcaaatta ctcgccactc gccgtccttc
    17761 gaaatgcata tggcaaatga cacgctgaat tttatgccgt ttgagaaagg aacattgctg
    17821 gcgcaggacg gggaggaacg ttttaccgta acccatgatg tagagtatgt gttattccct
    17881 aatccgttgg tagcgttggg attacgtgcg ggattaatgc tcgaaaaaat aagctaatac
    17941 ttacccgcag aaatcattct gcgggtatta agttgctttc ttataaatta atacagatta
    18001 atcctggtta tttgctttat ttatcaccag tcatccggta tagttcttca taatctctgc
    18061 aaaatcatcg tgttgtaata ttctctcatc actctccatc aaattttctt tttttctcca
    18121 taattggcgc aaaagtgttt tttacacttt cattgtttta ccgttgctct gattaattga
    18181 cgctaaagtc agtaaagtta atctcgtcaa cacggcacgc tacttaagaa agccgtaata
    18241 aataactgaa aggaaggata tagaatatgc gtaaattaac tgcactgttt gttgcctcta
    18301 ccctggctct tggcgcggct aacctggccc atgccgcaga caccactact gcagcaccgg
    18361 ctgacgcgaa gccgatgatg caccataaag gcaagttcgg tccgcatgag gacatgatgt
    18421 tcaaagacct gaacctgacc gacgcgcaga aacagcagat ccgcgaaatc atgaaaggcc
    18481 agcgtgacca gatgaaacgt ccgccactgg aagaacgccg cgcaatgcat gacatcattg
    18541 ccagcgatac cttcgataaa gcaaaagctg aagcgcagat cgcaaaaatg gaagaacagc
    18601 gcaaagctaa catgctggcg cacatggaaa cccagaacaa gatttacaac atcctgacgc
    18661 cggaacagaa aaagcaattt aatgctaatt ttgagaagcg tctgacagaa cgtccagcgg
    18721 caaaaggtaa aatgcctgca actgctgaat aatctttcag ccaaaaaacg taagaccgcc
    18781 ggtcttgtcc actaccttgc agtaatgcgg tggacaggat cggcggtttt cttttctctt
    18841 ctcaaacaga tgcaaaaaaa gcctgatcgc cactaacgta aatcacccga gttgccgata
    18901 accatccacg gttacctgaa cgataacaat gaaaagaacg ggccgggagc gggtatggaa
    18961 tactttgata tgcgtaaaat gtcggtgaat ctgtggcgaa acgctgccgg tgaaacgcgc
    19021 gaaatttgca ctttcccacc ggcaaaacgt gatttttact ggcgtgccag cattacatcc
    19081 atcgcggcga atggggagtt ttctttgttt cccggtatgg aaaggatagt gacgttgctg
    19141 gaaggcggcg agatgttcct tgaaagcgca gaccgcttta accatacctt aaaaccgtta
    19201 caaccttttt cctttgcagc ggatctggtg gtaaaagcga aactgacggc agggcagatg
    19261 tcgatggatt tcaacatcat gacacgcctg gatgtctgta aggcgaaagt cagaattgcc
    19321 gaacgtacgt ttaccacctt tggttcacgc ggcggggtgg tgtttgtaat aaacggggcg
    19381 tggcaattgg gcgacaaact gctcactacc gaccagggcg cgtgctggtt cgatggcagg
    19441 cacaccctgc ggcttttgca accgcaaggt aaactgttgt tcagtgaaat caactggctt
    19501 gctggtcatt caccggatca agttcagtaa tttcatagtt accggaaagc agcggcttac
    19561 aaagaatttt ataaccgtcg tgatcaaacc cggcgggtgc ccgaattaac gttgtcgctt
    19621 ctttcagcga attaaccgcc cccagccaca gccagttttg aataatatga tattgagtca
    19681 tttccgggtg ctgttctttc aatgccaccg cgccttgcca aggccaacac accacccgca
    19741 aacgctccag agcctggcgc aagcgtagcg catgttcctc atggctctct ttaccgcaac
    19801 acgccccggc gcaacgtttt aatgcagaac gaaaacatgc acgaccgcgg cttaacggtt
    19861 ccagtcccaa caggccataa caaagttttt gttcatcggc gatggtctgc aatgcttgca
    19921 aagctgcgcg cctattggca aacaggccaa acaggttgga ggctcgtgaa aaatccacct
    19981 ctttggcata caccacatca acacgctttt catttaattg cagggcgcag agctggcgat
    20041 tgcggcgcaa gcgtttatta aacagcggct gctgctcttt gattaatcgc gcttcaagga
    20101 gtagagcgcc gatttcaccg gcggtacata tccagctgat gcgtcgggat tgtcgcagca
    20161 tggcggcttc atccggggtg cgtaaatgag aaagcacccg actgcggata ttaacgcttt
    20221 tgccgatata gagcggcatg gtgtcacttt cgccatgaaa cagatacacc cctggtcggg
    20281 tgggtaagtc attaaggaat gaacgtaaat gttcgggata ttcataaatt gccgcagctt
    20341 caaattcgag ccgcggagaa gttaaacgcc gtaccactgt tgactccgaa tgctggttat
    20401 ctatccagtg taacaggcgg ggagcgatta aaaaagaggc gggttgatga caaaggttac
    20461 accgtctgac gtgctacgcc tgtcaggcct attcgactcc tgcaatgtat tgaatttgca
    20521 tagttttgta ggtcg
//
LOCUS       DAOFSV010000074        20438 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.074, whole genome shotgun sequence.
ACCESSION   DAOFSV010000074 DAOFSV010000000
VERSION     DAOFSV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.074"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            74..226
                     /gene="hokA"
                     /locus_tag="QBH27_002832"
     CDS             74..226
                     /gene="hokA"
                     /locus_tag="QBH27_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDQ7015126.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQENYELAAFL
                     ACKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="QBH27_002833"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="QBH27_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDQ7015127.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="QBH27_002834"
     CDS             complement(907..1197)
                     /locus_tag="QBH27_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDQ7015128.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1631..2341
                     /locus_tag="QBH27_002835"
     CDS             1631..2341
                     /locus_tag="QBH27_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDQ7015129.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="QBH27_002836"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="QBH27_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDQ7015130.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKVEKNCVNPHVVD"
     gene            complement(3469..4128)
                     /locus_tag="QBH27_002837"
     CDS             complement(3469..4128)
                     /locus_tag="QBH27_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDQ7015131.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4281..6614
                     /gene="bisC"
                     /locus_tag="QBH27_002838"
     CDS             4281..6614
                     /gene="bisC"
                     /locus_tag="QBH27_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDQ7015132.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTVTAFEPPASS"
     gene            complement(6583..7023)
                     /locus_tag="QBH27_002839"
     CDS             complement(6583..7023)
                     /locus_tag="QBH27_002839"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ7015133.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7020..7583)
                     /gene="tag"
                     /locus_tag="QBH27_002840"
     CDS             complement(7020..7583)
                     /gene="tag"
                     /locus_tag="QBH27_002840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDQ7015134.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7741..8439
                     /locus_tag="QBH27_002841"
     CDS             7741..8439
                     /locus_tag="QBH27_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ7015135.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8668..9876
                     /locus_tag="QBH27_002842"
     CDS             8668..9876
                     /locus_tag="QBH27_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015136.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10200..11891
                     /gene="eptB"
                     /locus_tag="QBH27_002843"
     CDS             10200..11891
                     /gene="eptB"
                     /locus_tag="QBH27_002843"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDQ7015137.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            11983..12059
                     /locus_tag="QBH27_002844"
     tRNA            11983..12059
                     /locus_tag="QBH27_002844"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12017..12019,aa:Pro,seq:cgg)
     gene            12803..14410
                     /gene="dppA"
                     /locus_tag="QBH27_002845"
     CDS             12803..14410
                     /gene="dppA"
                     /locus_tag="QBH27_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDQ7015138.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14717..15736
                     /gene="dppB"
                     /locus_tag="QBH27_002846"
     CDS             14717..15736
                     /gene="dppB"
                     /locus_tag="QBH27_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDQ7015139.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15746..16648
                     /gene="dppC"
                     /locus_tag="QBH27_002847"
     CDS             15746..16648
                     /gene="dppC"
                     /locus_tag="QBH27_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDQ7015140.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16659..17642
                     /gene="dppD"
                     /locus_tag="QBH27_002848"
     CDS             16659..17642
                     /gene="dppD"
                     /locus_tag="QBH27_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015141.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17639..18643
                     /gene="dppF"
                     /locus_tag="QBH27_002849"
     CDS             17639..18643
                     /gene="dppF"
                     /locus_tag="QBH27_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDQ7015142.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18673..19944)
                     /locus_tag="QBH27_002850"
     CDS             complement(18673..19944)
                     /locus_tag="QBH27_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDQ7015143.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
BASE COUNT         4900 a         5026 c         5367 g         5145 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acaggaggct gggatgccgc agaaatatag attactttct ttaatagtga tttgtttcac
      121 gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaacagga
      181 gaattatgag ctggccgcgt ttttagcctg caaattgaaa gagtaagaat cttcggcggg
      241 aagttattcc cgcctttctt acggcgttac gcattctcat tacacccaaa tttattcttc
      301 acaaaaataa taatagattt tattacgtga ccgattattt atttcctgaa aaacaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacacg
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaat tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cacgcattgg tgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtt atcaggaccg taaacagaaa taaagtggat
      901 aaaagcctat tccatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcgtaactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaaacag acacagcggg gtgtagtcag ggcatactct gactatgatt cgattcagcc
     1321 agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac
     1381 cactcccggt ttgtaaagat ttcgaaaatc taacgttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcaa tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt atgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gttgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaacggttc
     1981 gctgggtgtg ctggcgcaac agctgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgttaaaa cagagtgatg acctgaaacc ggtctttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttactcctcg cagtcgcggc ttagtccacg
     2401 acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcattaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtaat
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcccct ttctgcaatg cagcaatcag tgcattttca
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tccgttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcgacgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatactc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg tgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatcaac attctcattt gaacccagca agccttcagc ttcggcaaaa
     3241 attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ctacctggtg aacggtgaaa
     3301 tgttcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctgacat gaaagggatt acagtgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgact
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagtac
     3721 cattgccacg ccggtcaggg tgttagcgcc cgccggtttc agggttgcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataaccaac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatgagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gccaacgaga gagcccagac ctgcgccgat
     4021 agcagattta cctgcttcgc gttcgccggt gtaagggtta gttgtacagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
     4141 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgagcatga
     4201 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     4261 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     4321 cccactgggg gcctatgctg gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg
     4381 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     4441 atacgcgggt gcgatttcca atggtgcgca aaggctttct tgcgtcaccg gaaaatccgc
     4501 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggagctta
     4561 ttcaccatca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     4621 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     4681 tggcgttggc aggcggttat actgggcatc tgggggatta ttcgaccggc gcggcacagg
     4741 cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc agttggccgc
     4801 tggtgctgga acatagcgat gtcgtggtgc tgtggagtgc taacccactc aatacgctga
     4861 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca ctgcgtgaca
     4921 gcgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
     4981 ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgttgatg ctggggatcg
     5041 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag
     5101 gttatgctgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg aaaaacgccg
     5161 aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
     5221 tccaccaaaa tacaaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
     5281 agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc ggcacacccg
     5341 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cgccgcgctg
     5401 cggtgctctc ttccatgcag ggcagcttgc cgggtggcac cgatgcggta gataaaatcc
     5461 ctgtcgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cataacggta
     5521 tggaccgaca tttcccggat attcgtttta tctggtgggc gggtggtgcc aactttactc
     5581 atcatcagga taccaatcgc ctgatccgtg cctggcaaaa accggagctg gtggtgatct
     5641 ccgaatgctt ctggacggct gcggcgaaac acgcggatat cgttctgcct gcgaccacct
     5701 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
     5761 tgaagcaagt ggtgccgcca cgcgatgaag cgcgtaatga ttttgatgtt tttgccgagt
     5821 tgagtgaacg ttgggagaag ggcggttatg cacgttttac ggaaggaaaa agtgagctgc
     5881 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
     5941 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
     6001 acagtgagcg gtttattcgc ttcgccgatt ttcgccgcga tccgcaggcg catccattaa
     6061 aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac gcttatccgg
     6121 attgcccggg gcatccaatg tggctggcgc cggacgaatg gcagggtaat gccgaaccgg
     6181 aacagttgca ggtactttct gctcatccgg cgcatcgtct gcacagccag ctgaattaca
     6241 gttctctgcg cgaattgtac gcggtggcaa atcgtgagcc tgtcaccatt catcctgacg
     6301 atgcccagac gcgcggcata caagatggcg atactgttcg gctgtggaac tcacgcgggc
     6361 aaattcttgc cggcgcggtc attagcgagg gaattaaacc tggcgtgatt tgtattcacg
     6421 aaggcgcatg gccggatttg gatttaaccg ctgacggcat ttgtaaaaac ggcgcagtga
     6481 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
     6541 cgctggcatg gctggaaaaa tacaacggtc cggaactgac agttacagcg tttgaaccac
     6601 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     6661 gtgaaaaccc tgcgcatggt agaaatctat cgccggttga tttttttgat aaacctccag
     6721 catcagatgg ggatggcgct gctgcacata ctgcatcagc gccttaccaa taccgcgcct
     6781 gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa tgctgacaaa
     6841 accgagaagc ttaccgtctt cttcccagac ccagttttgc gcgttggcaa gatacgcatc
     6901 ccgcaccagc ggaatgcagt cacgccagta actcgatttt ataaagggat gcccccaggt
     6961 tgtactttcc agccacagtt cgaggagcgc ggggagttct gaacgttgcg cttcccgaat
     7021 catggtttat ttcccggatg gcaacagcag ccaaccacat gatcattcac cagcccacat
     7081 gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg tttttttagt
     7141 gccttagata gggcgtcgga ggcgggtgta gatgtgggga tttcgctcaa cgttgtggct
     7201 tgtgtcacct gtggttgatg attgacaaac gaccagacaa agtcggcaaa cggttcgccg
     7261 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttcccacga
     7321 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgcggca
     7381 actttcaccg gatcgaactg atgaaagcat gcgcgatagt tttcgcgttt tttgaggacg
     7441 gtgatccacg ataacccagc ctgctgccct tccaggcaga tcatttcgaa cagtttttta
     7501 ctgtcagttt caggcacgcc ccattcatta tcatggtagg caatataaag cgggtcctga
     7561 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     7621 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     7681 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     7741 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgcttgcgag cgtggtaatc
     7801 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
     7861 cccggacatt ccacagagcg ttacacctgg gatagcgatc atcaacctga ttacaacgat
     7921 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     7981 gaagaaaccc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     8041 gagcaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
     8101 tataacgagt ttggcgacag tactaccacg ctggccgatc cgttatggca tgccagcgtg
     8161 agtaccttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
     8221 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     8281 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     8341 aatcaaaata ttgctgccta tgctgcgcta tctcaggcag aaaataccac taacaatagc
     8401 gactatctgt atacgatggg ggttagtgcc agattttaac gttatagata caattgaaac
     8461 ccataaataa caactaatgt ggcgatagtg gggactaact tactaaataa taaatttggc
     8521 gaataattgt cgagtcattc attcctgaac taatgcattt cattccgttc tgatggcatt
     8581 tcatgccgtt tttccccagg cataaagcgc acttcgttat ggttgtcagc agagattttt
     8641 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tactcgctgg
     8701 ctgacactca tcggtactat cattacccag tttgctctgg ggtcggttta tacctggagt
     8761 ctgtttaacg gtgcgctttc tgccaagctg ggggctcctg taagccaggt tgctttctct
     8821 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa
     8881 cgttttggtg ttaaacgcgt cactatggct tccggcattt tgctgggatt aggtttcttc
     8941 ctgacagcac attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gttggttggt
     9001 ctggcggatg gtgcgggtta cctgttgacg ctctctaact gcgtgaagtg gtttccggag
     9061 cgtaagggac ttatctctgc ttttgctatc ggttcttatg gtctgggcag cctgggtttt
     9121 aaatttatcg acacgcacct gctcgaaacg gtgggtctgg aaaaaacctt tgttatttgg
     9181 ggcgcgattg tactggtgat gattgtcttt ggcgcaacgt taatgaaaga tgcgccgaag
     9241 caggaagtga aaaccagcaa tggtgtggtg gagaaggact acaccctggc agagtcgatg
     9301 cgtaaaccgc agtactggat gttagcggtt atgttcctga ctgcgtgcat gagtggtctg
     9361 tatgtgattg gtgtagcgaa agatatcgct caaagtctgg cgcatcttga tgcaatttcc
     9421 gcagccaatg ctgtgacggt tatttccatc gccaaccttt ctggtcgtct ggtgctgggc
     9481 attctgtctg acaaaatcgc ccgtatccgt gttatcacca ttggtcaggt gattgcgctg
     9541 gtgggtatgg cggccctgct gtttgcacca ttgaatgcag tgacgttctt tgcagcgatt
     9601 gcctgtgtgg catttaactt tggtggcacg attacggtgt tcccgtcact ggtcagtgag
     9661 ttcttcggcc tcaataacct ggcgaaaaac tacggcgtca tttacctcgg tttcggtatc
     9721 ggcagcattt gtggttcgat tatcgcttca ctgtttggcg gattctatgt gactttctac
     9781 gtgattttcg ccctgctgat tctgtcactg gcgctttcta cgaccattcg ccagccagag
     9841 cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgtgag ctactgggct
     9901 tcagcttcag tgtgcatcaa acgcatcaac gaaatgtata aacaataacg cgtaaaattt
     9961 gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct tttcaaactt
    10021 gcatttttgt aaatttgtgc ttcgtgcaca ctctttcccc acactttttc cctttgctgt
    10081 ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac ttttcccctg
    10141 cctggcctga aaagccacta agcagggtgt tatcacctgt ttgtccaggg tttgtttgca
    10201 tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca atctatattg
    10261 gcctttttat gaatggcgcg gttttttacc gccgctttgg cagctatgcg cacgatttta
    10321 ccgtctggaa aggcatttct gctgttgttg aactggccgc caccgtactg gtgaccttct
    10381 ttttactgcg tctgctttcg ctgtttggcc gccgcagctg gcgtattctg gcatcgctgg
    10441 tggtgctctt ttctgcaggt gccagctatt acatgacctt ccttaatgta gtcattggtt
    10501 atggcatcat cgcatccgtc atgaccaccg atatcgacct gtcaaaagaa gttgttggtc
    10561 tgaactttat tctctggtta atcgccgtaa gcgcgttgcc tcttatcctt atctggaata
    10621 accgctgtcg ttacaccttg ctccgacaac tgcgaacccc agggcaacgt attcgcagcc
    10681 tggcggtcgt cgtactggca ggtattatgg tttgggcacc gattcgtttg ctggatatcc
    10741 agcagaagaa agtggagagg gcgacaggtg ttgatttgcc gagttatggc ggcgtcgtcg
    10801 cgaactctta tctgccatca aactggcttt cggcattagg tctttatgcc tgggcgcggg
    10861 tcgatgaatc ttccgataac aattctctcc ttaaccctgc gaagaagttt acgtaccagg
    10921 ctccacaaaa cgttgatgat acatatgtcg ttttcattat tggtgaaacc acgcgctggg
    10981 atcatatggg cattttcggc tatgaacgca ataccacacc gaaactggct caggagaaaa
    11041 atctggcggc gttccgtggt tactcctgtg atactgcaac caaactctca ctgcgttgca
    11101 tgtttgtacg tcagggtgga gcagaagata atccgcagcg cacattaaaa gaacagaaca
    11161 ttttcgcggt attgaagcag ttaggattca gttctgatct ctacgctatg caaagcgaaa
    11221 tgtggttcta cagcaatacg atggcggaca acattgctta tcgtgagcag attggtgcgg
    11281 agccacgtaa ccgtggcaaa ccggtagatg atatgttgct ggtagacgaa atgcagcaat
    11341 cgctggggcg caacccggat ggtaagcatc tgatcattct gcataccaaa ggctcacact
    11401 ttaactacac ccagcgttac ccgcgcagct ttgcgcagtg gaagccggaa tgtattggtg
    11461 ttgatagcgg ctgtaccaaa gcgcagatga tcaactcata cgacaactcg gtgacctatg
    11521 tggatcactt tatttccagc gtgattgatc aggttcgcga taagaaagcg attgtgttct
    11581 atgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc acgccgcgtg
    11641 aactggcacc gccggaacag ttccgtgtgc cgatgatggt ctggatgtcg gataaatatc
    11701 tggaaaatcc ggtcaatgcg caggcatttg cgcagctgaa aaaagaagcc gacatgaaag
    11761 tgccacgccg tcatgtagag ctgtacgaca ccatcatggg ttgtcttggc tatacttcac
    11821 cggatggtgg aattaacgaa aacaacaact ggtgccacat cccgcaggca agagaggcgg
    11881 cggctaacta agaggcctgc tgactttctc gccgatcaaa aggcattttg ctattaaggg
    11941 attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt ggcgcagcct
    12001 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat caccgaccaa
    12061 attcgaaaag cctgctcaac gagcaggctt ttttgcatct gtaactcatg aggaagagaa
    12121 cccccggggg cggggaggtt cgactcgacg taggcctgat aagacgcacc agcgtcgcat
    12181 caggcaaatg taccggccca ttgtcggatg cggcgcgacc gccttatccg atctacacac
    12241 cacacatttc cctgtcaaat ttgcgcccca tatctcacta tccattaacg gatttgtgac
    12301 agaaaccatt gtcttttttt atatttgctt aaatcttttt ttgcgttact tatggctggc
    12361 gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag atatccacct
    12421 ttacctcaga tttttttcat taaatgcata aaacttaatc accaaatgtt gctaaatatt
    12481 aagctgattg ttctggttgt gttggcgtag cacaaatttc tctgctgcaa atagctgttt
    12541 gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt
    12601 attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt tatggaggat
    12661 ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg ttgacccgac
    12721 cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt aaaacaacaa
    12781 acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga tgctgaagct
    12841 tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta
    12901 ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg gcaccaccta
    12961 tgacgcctct tccgtaccgc tttataaccg tctggttgaa tttaaaatcg gcaccactga
    13021 agtcatcccg ggcctcgctg aaaagtggga agtcagtgaa gacggtaaaa cctatacctt
    13081 ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga cgcgcgaact
    13141 gaatgccgat gatgtagtgt tctcgttcga tcgtcagaaa aacgcgcaaa acccgtacca
    13201 taaagtctct ggcggcagct acgaatactt cgaaggcatg ggcttgccag agctgatcag
    13261 cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgcc cggaagcgcc
    13321 gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat atgctgacgc
    13381 gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggta ccggcccgtt
    13441 ccagttacag cagtaccaga aagattcccg tattcgctat aaagcgtttg atggctactg
    13501 gggcaccaaa ccgaagatcg atacgctggt cttctctatt actcctgacg cttccgtgcg
    13561 ttacgcgaaa ttgcagaaga acgaatgcca ggtgatgccg tacccgaacc cggcagatat
    13621 cgcccgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc tgaacgtcgg
    13681 ttatctctcg tataacgtgc agaaaaaacc actggatgac gtgaaagttc gccaggctct
    13741 gacctacgcg gtgaacaaag acgccatcat caaagcggtt tatcagggcg cgggcgtatc
    13801 agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc aggactacac
    13861 ttacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa aaggtttctc
    13921 catcgacctg tgggcaatgc cggtacaacg tccgtataac ccgaatgctc gccgcatggc
    13981 ggagatgatt caggcagact gggcgaaagt gggcgtgcag gccaaaatcg tcacctacga
    14041 atggggtgag tacctcaagc gtgcgaaaga tggcgagcat cagacggtaa tgatgggctg
    14101 gactggcgat aacggggatc cggataactt cttcgccacc ctgttcagct gcgccgcctc
    14161 tgaacaaggc tccaactact caaaatggtg ctacaaaccg tttgaagatc tgattcaacc
    14221 ggcgcgtgct accgacgacc acaataaacg tgttgaactg tacaaacaag cacaggtcgt
    14281 gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg aaccggtacg
    14341 caaagaagtt aaaggctatg tggttgatcc attaggcaaa catcacttcg aaaatgtctc
    14401 tatcgaataa ttaaagccat acaagactga tggcaaaggc aaaactgcct gatgcgctcc
    14461 gcttatcagg cctacgaaaa ctctgcaatg tattgagttt gcacgatttt gtaggtcgga
    14521 taaggcgttt gcgccgcatc cggcataaac aaagcgctct ttgtcaacaa tctgcatacc
    14581 cggtggcgct gtgcctctgc gtgcagcgcc atccggcagc aatacttcat tccctgtccc
    14641 acaagggcgg gaattgattt gtgagcaata cagacacgca gttccaggct gcgggtcact
    14701 atagagaatc cgggttatgt tgcagtttat tctccgacgt ttgggactcg tcatccccac
    14761 gtttatcggt attacccttc tcacatttgc ctttgtccac atgatcccgg gcgatccggt
    14821 gatgatcatg gcgggcgaac gtgggatctc cccagagcgt cacgcgcaat tgctggctga
    14881 actcggctta gataaaccga tgtggcagca gtatctccat tacatttggg gcgtaatgca
    14941 cggtgattta ggcatttcca tgaaaagccg aattccggta tgggaagagt tcgtgccgcg
    15001 ctttcaggcc acgctggaac ttggcgtctg cgcgatgatt tttgcgaccg cagtcgggat
    15061 tccggttggt gtgctggctg cggttaaacg tggttccatt ttcgatcaca cagcggttgg
    15121 cctggcgctg accggctact cgatgccaat tttctggtgg ggcatgatgt tgatcatgct
    15181 ggtttcggtg cactggaacc tgacgcctgt ctccggtcgc gtgagcgaca tggtgttcct
    15241 cgatgattcc aatccgttaa ccggttttat gctgatcgac accgccatct ggggtgaaga
    15301 cggtaacttt atcgatgccg tcgcccatat gatcctgccc gccattgtgc tgggtactat
    15361 tccgctggcg gtcattgtgc gtatgacgcg ctcctcgatg ctggaagtgc tgggcgaaga
    15421 ttatatccgc accgcgcgtg ccaaaggtct tacccggatg cgggtgatta tcgtccatgc
    15481 gctgcgtaac gcgatgctgc cggtggtgac cgttatcggc ctgcaggtgg gaacattgct
    15541 ggcgggggcg attctgactg aaaccatctt ctcgtggccg ggtctggggc gctggttgat
    15601 tgacgcactg caacgccgcg attatccggt agtgcagggc ggcgtattgc tggtggcgac
    15661 gatgattatc ctcgtcaacc tgctggtcga tctgctgtac ggcgtggtga acccgcgtat
    15721 tcgtcataag aagtaagggg gcatcatgtc acaggttact gaaaataaag tgattagcgc
    15781 accggtgccg atgaccccgt tacaggagtt ctggcactat tttaaacgca acaaaggcgc
    15841 ggttgtcggg ctggtttacg tcgtcatcgt gctgtttatc gcgatctttg ccaactggat
    15901 tgcaccctat aacccagcgg aacagttccg cgatgcactg cttgccccgc ctgcctggca
    15961 ggaaggcggc agcatggcgc acttgctggg cactgatgac gtggggcgtg atgtgctgtc
    16021 gcggctgatg tacggcgcgc gcttgtcgct gctggttggc tgtctggtag tggtgttatc
    16081 gctgattatg ggcgttattc tcggcctgat cgctggttac tttggcggtc tggtcgataa
    16141 catcatcatg cgcgtggtcg atatcatgct ggcgctgcca agtctactgc tggcactggt
    16201 gctggtggcg attttcggcc cgtcgattgg taacgccgcg ctggcgctaa ccttcgttgc
    16261 gctgccgcat tatgtgcgct taacccgcgc cgccgtgctg gtggaagtta accgcgatta
    16321 cgtcaccgcg tctcgcgtgg cgggtgccgg ggcaatgcgc cagatgttta ttaacatctt
    16381 cccgaactgc cttgcgccgc tgattgttca ggcgtcgctc ggtttctcta acgccattct
    16441 cgatatggct gctcttggtt tcctcggcat gggcgcgcag ccgccaacgc cggagtgggg
    16501 caccatgctc tctgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc
    16561 gggtctggcg atcctgctga cggtgctggc atttaacctg atgggtgacg gtctgcgtga
    16621 cgcgctcgat cccaaactga agcagtaaga ggttcgagat ggcgttatta aatgtagata
    16681 aattatcggt gcatttcggc gacgaaagcg caccgttccg cgctgtagac cgcatcagct
    16741 acagcgtaaa acagggtgaa gtggtcggga ttgtgggtga gtccggctct ggtaaatcgg
    16801 tcagttcgct ggcgattatg gggctgattg attatccggg ccgcgtaatg gcggaaaagc
    16861 tggagtttaa cggtcaggat ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg
    16921 gtgccgaagt ggcaatgatc ttccaggacc cgatgaccag ccttaacccg tgctacaccg
    16981 tgggtttcca gattatggaa gcgattaagg tgcatcaagg cggcaacaaa agtacccgcc
    17041 gtcagcgagc gattgacctg ctgaatctgg tcggtattcc cgatccggca tcgcgtctgg
    17101 atgtttaccc gcatcagctt tccggcggca tgagccagcg cgtgatgatc gccatggcga
    17161 ttgcctgtcg gccaaaactg ctgattgccg atgaaccaac cactgcgctg gacgtgacca
    17221 ttcaggcgca aattatcgaa ctgctgctgg agctacagca gaaagagaac atggcgctgg
    17281 tgttaattac ccatgacctg gcgctggtgg cggaagcggc acataaaatt atcgtgatgt
    17341 atgcaggcca ggtagtagaa acgggcgatg cgcacgctat cttccatgcg ccgcgtcacc
    17401 cgtatactca ggcattgctg cgtgcgctgc cggaatttgc tcaggacaaa gaacgtctgg
    17461 cgtcgttgcc aggtgtcgtt cccggcaagt acgaccgccc gaacggctgc ctgcttaacc
    17521 cgcgctgccc ctacgccact gacaaatgcc gtgctgaaga accggcgctg aatatgctcg
    17581 ctgacgggcg tcagtccaaa tgccattacc cacttgatga tgccgggagg ccgacactat
    17641 gagtacgcat gaggccaccc cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca
    17701 ttatccggtg aagaaaggca tgttcgcgcc ggaacgtctg gtgaaggcgc tggatggcgt
    17761 ttcgtttaac cttgaacgtg gcaaaacgct ggcagtagta ggtgaatctg gctgcggtaa
    17821 atcgacccta ggtcggttgc tgacgatgat tgaaacgccc accggcggcg aactgtatta
    17881 ccaggggcag gatctgctca agcacgatcc gcaggcgcag aagctacgtc ggcagaaaat
    17941 ccagattgtc ttccagaacc cttacggttc gctgaatccg cgtaaaaaag ttgggcagat
    18001 tctcgaagag ccgctgctga tcaacaccag cttaagcaaa gaacagcgtc gggaaaaagc
    18061 cctgtcgatg atggcgaaag tcggcctgaa aaccgagcat tatgaccgct atccgcatat
    18121 gttctccggc ggtcagcgtc agcgtatcgc cattgcccga ggtctgatgc tcgacccgga
    18181 tgtggtgatt gccgatgagc cggtttccgc gctggacgtc tcagtgcgtg cgcaggtgct
    18241 gaatctgatg atggatttgc agcaggaact ggggctatct tatgtcttta tctcgcacga
    18301 tctgtcggtg gtggagcaca ttgctgatga agtgatggtg atgtacctgg gccgctgcgt
    18361 ggagaagggg acgaaggacc aaatcttcaa taacccgcgc cacccgtaca ctcaggcgct
    18421 gctctccgcg acaccacgcc tgaacccgga cgatcgtcgc gaacgcatca aacttaccgg
    18481 tgaactgcca agcccgctga atccaccgcc aggctgcgcc ttcaacgccc gctgtcgtcg
    18541 gcgcttcggc ccctgcatcc agttgcagcc gcagctaaaa gactacggcg gtcaactggt
    18601 agcttgtttt gccgttgatc aggatgaaaa tccgcagcgt taactcacaa aaccggggcc
    18661 atgcgctccg gtttactcaa tcaacttcaa cagtggcgac aagcataaca aaatgccgta
    18721 cagcagaatc atccaggctt taaacccgcg aagtttttcc agttgtgaga ctttatagat
    18781 caggaaaaac ggaatgaggc acgacacaat gccatataac gggcttccca actgaaagaa
    18841 caccagtacc gatacacgaa acgaaaccca aatcgtcaac gtaatgacga taaaagtgca
    18901 gatcgccaga gtcagcacgc gtgagttaat tttcctggta tcaataattc ggcttaacag
    18961 attgagaata atgcctttaa tggcctcgtg gaaaccgaga taaatgccaa agaatgcagt
    19021 cagtacggca aagatattca gcaccgtaga ggtgatatga atgatatgcc cagggatcac
    19081 ctgcgcggcc agtgccagtg ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg
    19141 gctaattgag aaggtaaacg aaaaggcaaa aaacaggatc accgcgatga gcgtgacata
    19201 actaattcgg tgggtacgca gcgcgagccg ggttgccagt actttatccg cttcccgttt
    19261 acgataggca atattcattg ggttaagtac ctgaataaat accgcggaaa agaagcaaaa
    19321 tggaatagta agcaggacat cgcggaaaaa gtcggaggct tgcgggaagg cggttatatt
    19381 ggcgaaattc cagtgcggga tcatcgcaaa accgaacacg acaataatcc ctactttgac
    19441 caccaccatt ggtccggaaa tcttaaacag taatcgttca ccaccagacg caatcgccac
    19501 cagtacggcg aaaatggcga ctttataaaa tagagattga gaaagatcgg catcggttaa
    19561 accgaaggtt ttcaggtacg aggcgctgtc gaaaaccacg gagagagagt agataaaaat
    19621 cccgtggata atcatcaaaa agtagataac cccgaggaaa attccccagt tcttccccag
    19681 gtaatgactg ataatatcgg tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa
    19741 ataaatgtcc tgtactaccc aggtggcagg ataagcaatg atcgctgcgg taataaatac
    19801 ccaaattccc ttcaagccaa tttgtactgg catcagcacg gttccggcac caatcgccat
    19861 gcctatgcat aataaaaccc agccgaagtc gtagcgtgta aacggcaatt tctgatcgtg
    19921 tttcggtagt gtgttgtgct gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa
    19981 tgaataaacc cagtttctct tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa
    20041 aatgaggcaa cagattcact ggggaggtcg cgaggaggcg caattctaca aaattgcgca
    20101 tttcactact agtctggagt cagcccggat atgagctgca actattgcaa ataaagaaat
    20161 taagctggaa ggcatcacgc aatgaggaag attgccgggt gctgacggca aaaagcaatc
    20221 gtaaaatcag cggcaagccg gaagggttcc ggtgaggcgc aatgttgcgg gggctttatc
    20281 cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac
    20341 aacatcacgg gggccattct tgcatctaga ccattcaaga atagccgcga aacgttgtca
    20401 ttacaacaca ggcggctata tgacgctcgc agagctgg
//
LOCUS       DAOFSV010000075        20429 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.075, whole genome shotgun sequence.
ACCESSION   DAOFSV010000075 DAOFSV010000000
VERSION     DAOFSV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.075"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(8..793)
                     /locus_tag="QBH27_002851"
     CDS             complement(8..793)
                     /locus_tag="QBH27_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7015144.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGDGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(826..1623)
                     /locus_tag="QBH27_002852"
     CDS             complement(826..1623)
                     /locus_tag="QBH27_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7015145.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAERVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLVHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            complement(1677..2744)
                     /locus_tag="QBH27_002853"
     CDS             complement(1677..2744)
                     /locus_tag="QBH27_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7015146.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGNIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     ETLGNTGFATLGSGTFLGIYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            complement(2762..4306)
                     /locus_tag="QBH27_002854"
     CDS             complement(2762..4306)
                     /locus_tag="QBH27_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015147.1"
                     /translation="MLPQEIAPPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNK
                     VNVLIGENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNSTHQAEKHGISIIFQELN
                     LFPNMNVMDNIFMANEFFQKGKINEKYQYELAKSLLERLELNVDPYTQLGELGIGHQQ
                     LVEIARALSKDTRVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELME
                     IGDFITIFRDGRFISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTA
                     LHPSGGYKLNDVTFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETID
                     KCHFQARLKKGLALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESA
                     SVKEMILQLAIKVSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAK
                     TDVYQLVGKMAQQGLAVMFSSSELDEVMALADRILVMADGRITADLPRGEVTREKLIA
                     ASTPQD"
     gene            complement(4308..4928)
                     /locus_tag="QBH27_002855"
     CDS             complement(4308..4928)
                     /locus_tag="QBH27_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HDQ7015148.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGSITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            complement(4975..5910)
                     /locus_tag="QBH27_002856"
     CDS             complement(4975..5910)
                     /locus_tag="QBH27_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7015149.1"
                     /translation="MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANAFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            complement(6014..7534)
                     /locus_tag="QBH27_002857"
     CDS             complement(6014..7534)
                     /locus_tag="QBH27_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDQ7015150.1"
                     /translation="MFAGNKEFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAELALEL
                     NTLPASVDSTMGVYFVPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAVLESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALIPGTVADLKALLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            complement(7885..8835)
                     /locus_tag="QBH27_002858"
     CDS             complement(7885..8835)
                     /locus_tag="QBH27_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDQ7015151.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDASDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
     gene            9198..10028
                     /locus_tag="QBH27_002859"
     CDS             9198..10028
                     /locus_tag="QBH27_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ7015152.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEELKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALEELKDA
                     "
     gene            10021..10956
                     /locus_tag="QBH27_002860"
     CDS             10021..10956
                     /locus_tag="QBH27_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDQ7015153.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGTAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGITLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(10958..11617)
                     /gene="entD"
                     /locus_tag="QBH27_002861"
     CDS             complement(10958..11617)
                     /gene="entD"
                     /locus_tag="QBH27_002861"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDQ7015154.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPTGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDAGFLRYQIRNCQHQQMNISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(11792..14032)
                     /gene="fepA"
                     /locus_tag="QBH27_002862"
     CDS             complement(11792..14032)
                     /gene="fepA"
                     /locus_tag="QBH27_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDQ7015155.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            complement(14037..14237)
                     /locus_tag="QBH27_002863"
                     /pseudo
     CDS             complement(14037..14237)
                     /locus_tag="QBH27_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14275..15477
                     /gene="fes"
                     /locus_tag="QBH27_002864"
     CDS             14275..15477
                     /gene="fes"
                     /locus_tag="QBH27_002864"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDQ7015156.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     AIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTIGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            15480..15698
                     /gene="ybdZ"
                     /locus_tag="QBH27_002865"
     CDS             15480..15698
                     /gene="ybdZ"
                     /locus_tag="QBH27_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDQ7015157.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            15695..19576
                     /gene="entF"
                     /locus_tag="QBH27_002866"
     CDS             15695..19576
                     /gene="entF"
                     /locus_tag="QBH27_002866"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDQ7015158.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLFEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
     gene            19823..>20429
                     /gene="fepE"
                     /locus_tag="QBH27_002867"
     CDS             19823..>20429
                     /gene="fepE"
                     /locus_tag="QBH27_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDQ7015159.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKE"
BASE COUNT         4847 a         5276 c         5178 g         5128 t
ORIGIN      
        1 accattttta ccagcagaca aagccaccat cgaccaccag atccacaccc gtgcagaacg
       61 acgccgcatc gctggcaagg aacagcgctg gacccgccat ctcttccact ttcgccatcc
      121 gttgaatcgg cgtctggctt tcaaactccc gagtctgatg taccatttca ggtcgtgtat
      181 tcattggtgt cgcggtatat cctgggctga ttgagttcac gcggatcccc ttactcaccc
      241 attccatcgc aagactttta gataaatgaa tcacgccggc tttggaactg ttgtagtggg
      301 cctgttccag accacgattg acaataatcc cggacattga cgcaatattt ataatcgaac
      361 cgtctccgtt ctctaccatt aactcagctt ccgccttaca ggaattccac acaccagtca
      421 ggttgatatc gataacgcgt tgccattgct cgctttccat ctccagcgcc gggtttgcgt
      481 tagcgattcc agcggcgttt acggcaatat ccagacgacc aaaatgtgtt tttgccagag
      541 cgactgctgc gcgaagatct ctcaattccc gaacatcgcc cgtgtaatac aaggcttgtc
      601 cgccaagtgc ttcgatatga ttaaccgtct ctttgagtcc gccatcttcc cgtagatcaa
      661 aacacaccac tcgtgcgcct gcgctggcta aagccagagc gatcatttgt ccgataccgc
      721 tacctgcgcc agtgacaaag gcgacgcgat cgtggagatc aaacaattgt tgagcaaaat
      781 cttttgcgat cattttgata tctctcttag tagcccgcag gggcattacg acataatcag
      841 gccgccgtcg atgttgatgg tttgaccggt gagataacgc gcatcgtcag aggcgaggaa
      901 ggcaaccagt ccggcaacat cttcaggttt accggcacgc ttcatcggaa taccttccac
      961 ccattccgcc ataagctcgc ctttgccata acgcttgtca tcggtactga gaatttctcc
     1021 ccatacgcgg tcgttgtagt cccacatttc gctttcgata atacccgggc agaaggcgtt
     1081 aacagtgata ttccactgcg ctaactcgtg caccaggctt tgcgtaatgc cgatcacacc
     1141 cattttgctg gctgcatagt gtggggtata gataaacccc tgacgtccct ggccagaaga
     1201 ggtgttgatc aggcagccgt gattctgttt caccatatat ttggctgctt cccggcagca
     1261 gagccagact gccgtagtgt tcaccgccag cactttttcg aaatcagcag ccggcattcg
     1321 atcaaaataa tcgatggtga taatgcctgc attctgaatg gaaacatcga tgctgccaaa
     1381 acgtgcagcc gcttgttcat agagtgcctg tacttgcgct tcatctgtca catcgacttc
     1441 taatgacaaa atgtcagcct gataacattg gcgcagttgc tctgcagttt catgtacgcg
     1501 ttcagcgtta gaaaccatca ccagatttgc gccatcgcgg gcgaagcgtt cagcgatccc
     1561 ggccccaatt ccccggcatg ccccggtaat aactacagtc ttaccctgaa aattacgttg
     1621 catatttttt cccctgtata agccgggcaa tttaatgccc ggtggaatga tgttaattaa
     1681 ctggaaagcg gttttacggc aggtgcggcg gccatctttt cttcatggcg acgcatcaaa
     1741 atgactttgt tttgcaattt ttgctggaac tgatcgacca ccacggcggt gacgataacg
     1801 aggcctttaa tgaccatttg ccagaagtca ctgacaccca tcatcaccat gccatcggcg
     1861 aggaagacaa tgacaaaggc accgataatc gagccagaga cacgtccccg accacccgcc
     1921 agtgcagttc caccaagaac cgtggcgcca atagcatcca tttcgaacat attgccggtc
     1981 attgggtgag cagtttgcag ctgcgaggca acaatcaaac cgacaaatgc ggcgcataac
     2041 ccggagaacg catagacgaa cactttaact tttacgatgg ggacgcctgc cagtcgcgcg
     2101 gcagattcat tgccaccaat ggcatagata taacgaccga gcggcgtttt agtcgtgatc
     2161 cagtagccta aaatcaaaaa gccaatcatc agccagattg gtagataaat gcctaaaaac
     2221 gtgccagaac caagggtggc gaaacccgtg ttgcccagcg tttccatgcc gttgagattg
     2281 ggataagtgc tgccgtcatt aaacagcagg gcagagccac gagcgacgta catcataccg
     2341 agggtgcaga taaagggcgc gacaccaaag cgggtgatta ccgcaccatt caccagtccc
     2401 actaaaatgc caaataccgc cacgcagagg atcacttccg gcacgttgaa gaaaataata
     2461 ttgccgctcc acaatggcag gccgtttgtt agaagagccc cggcaaccat tccgcagatc
     2521 cccgctaccg cgccgacgga aagatcgatg ccgccggtca ggatcaccag cgtcattccg
     2581 attgccagca gcccggtaat cgcaacgtgt tgagtcatga tcaacaggtt agaggctgtc
     2641 aggaagttag gcaccatcac gctaaagaag gcgatcacta atagcagagc gataaacgtt
     2701 ctggccttca acaggtacat atagatcata tatttctgat tcataagaaa ttccagcctg
     2761 attaatcttg aggtgttgaa gccgcaatca gtttctctcg ggtgacttcg ccccggggga
     2821 gatcagcggt aatacggcca tcagccatca ccagaatgcg atctgccagt gccatgactt
     2881 catcgagttc tgaagaagag aacatgactg ccagcccctg ttgtgccatt tttcccacca
     2941 gctgatagac atctgttttt gcgccaacat caatgccgcg tgtgggttca tcaagaaaca
     3001 ccacctgtgg ctgggtcatc aacgctttgc caagtaccac tttttgctgg ttaccgccgc
     3061 tgagtgaggt aatagggagt tcggcatcgc tgactttgat cgccagttgc aggatcatct
     3121 ctttcacact ggcgctctct ttttgcggat tcagccattt ccaggcacgg cgaaaacctt
     3181 gcaggctgaa atcggatagc gtcatattgg actgaatcga catcatctga acgacgcctt
     3241 cgccctggcg atcttctggc accaacgcca gccccttttt gagccgcgcc tggaaatggc
     3301 atttgtcgat ggtttcacca ttaagctgga tcttcccact ctgacagggc atcaggccca
     3361 ccaatccctt aaatagttca gtacgtcctg cgcccagcaa gccgtaaatg ccgataactt
     3421 cgcctttatg caggctgaat gtcacatcat tgagtttgta accaccgctg ggatgcagcg
     3481 ccgttaaccc ttgtaccgcc agtacagtgt caccttgtgt agctggttga taatcgaaat
     3541 gttttttctt atcaccgacc atttgctcga tgatccatgg aatactggca tcgctcacgg
     3601 cgcgttcact aataaagcga ccatcacgga aaatggtgat gaaatcgcca atctccatca
     3661 actcttccag acgatgggag atataaatga tggtgacacc acggcgcttc agttgtgcga
     3721 tcacattgaa aaggactttt acttccgact ggctgagggc cgatgtcggt tcatccataa
     3781 tcagtacccg ggtatcttta gaaagggcac gggcaatctc caccagttgc tgatggccaa
     3841 tacctagctc tcccagttgg gtatagggat caacattcag ttccagtctt tctaatagag
     3901 atttggctaa ttcatattga tatttttcgt taatcttccc tttctgaaaa aattcattcg
     3961 ccatgaaaat attatccatg acattcatat tcgggaataa attcagctcc tgaaaaatga
     4021 tactaattcc atgtttttcg gcctgatggg tagaatttag ggtgacttct ttaccatcaa
     4081 gaatgatctt gcctgaggaa ggtgtttcaa cccccgccag cattttcatc attgtggatt
     4141 tacccgcacc gttttcacca ataagcacat ttactttatt gcgatagact cggtaattca
     4201 cgttatctaa cgccaccaca cccggataaa tacgcgataa gtcatgcgtt tctatgatta
     4261 caggtgattc atcagtctgg gggggggcaa tttcttgcgg caacatatta ctcgcctccc
     4321 gccctggcta ttttcgcagg cgttatatcc gggatagttt gcggaatatc ccatgcagaa
     4381 aatacgccat aaacctgaac cgtttccccg gttttaagct ttgccgactg aatcatattg
     4441 atggcttgct cattgatagc acgaccatat tcaccaaata aaacctgatc gttaaaatgc
     4501 tcatagcttg cacctttata tgcatctcgc agaagcgtgc cgcgaagagt tggccccgtc
     4561 tgcacgacaa tatttgtgcc gctgatatct gtgatggtca tttttccgct acgggatttc
     4621 gcatcaatat tggtgatgct accttcgacc ttaacgtaga aaatacacgg gttttcttcc
     4681 tgagaacgat agcccagcgt tttacaggca gcatcgacat ctttttctgc ctgtagcgcc
     4741 gccattaact ccgcaaccgg acgcgcattt tctaccacct gcggcacgat gtttttctgc
     4801 caggtctgat cgatatttgc catatgagga ttaggcgggg atttgagatc ggcaagttcc
     4861 tgctgcgaga cgatgcgaca tccacccagg ctcagaattg ccagcgccag ccagactttt
     4921 ttatccataa tattctcctc tgcgccctca ttgtgagggc gcaacgacct gggatcagga
     4981 tttgatattg aaatcctgga ctttatctgc gttatctttg gttatcagaa tgccgcggaa
     5041 catcacccgc tgtttggctg gtttaactcc tttttgcagg taattatcca gatcggtaac
     5101 accctgcgcg gcaatggctt gtgcttgtaa catcacggta gcctgcaatg attcggcttt
     5161 cacggcatcg cgctcatcgt tactgccatc aatgccgaca acaataacgt cattgcgatt
     5221 ggcggctttc agtgctgcga tagctcccag tgcaaccgga ccattaccac aaatcacccc
     5281 tttaacatcg ggatgggctt gcagaatact gtccattatt cgtttgccat caattaacgt
     5341 gcctttggca tcttgcttcg ccacgctgac catttcaggg aactggtcaa taacctggtg
     5401 gaaagatttc gagcgagtta cgcaattatt atcagcaaga ttacaggtga gttcggcata
     5461 ttttcctttc tctcccattt tctcgacaaa tgcgttggca acttccgagc cagcctggaa
     5521 gttattatgg gtaatttgca ccagtgcaac atcatccaca ggtatttcgc gattaattaa
     5581 tataacggga atacctgctt tttttgcttt ttcaatggct gcaacactgg cagtggagtc
     5641 ggcattatct aaaataatgc cctgaacttt tttaccaatt gcagtatcga tcagttcatt
     5701 ctgttttttg acatcttcac catgagaaag aatggtcgtt ttatatccca gttgctgggc
     5761 tttttcactg gcacctttcg cttccgaggc gtaataagga ttatccagtg aattgaccat
     5821 aatcataatg gttccttttt ctaccgcatg agcagaaaaa gaaagagcag tcagggtcgc
     5881 agcggttaac agtgttaaac gtaatttcat gacaatgttc tctctgtgtt gttattgtcg
     5941 ggtacggtga aacaacgtaa agcctgaatt atttatgggc cggtattctc cttacgttgg
     6001 ttagtcttgt tcgtcaaatt gacgatgccg gtttccataa tgttctgttt accgcatgac
     6061 gccactcctg ccatctcttt tgcagtcgct gatgacgcgc catgtccggt ttaaattcac
     6121 tgtgttcagt taataacgct tttaaatctg caacggtacc ggggataagc gctttgcggg
     6181 cgagcaggcc cgcaccaatg gctgaaagct ctggcacatc actgcacatc accgggcaac
     6241 ccagcagatc cgcctgatat tgcatcaacc agtcattttt ggtcgggccg ccatccacca
     6301 ttaacgccga taagtgaaaa tcatcatgct gttgcattgc ctccactaca tctgcaattt
     6361 gataagtgat tgattccagt acggcacgaa tcaaatgcgc gcgctttacg ccacggctta
     6421 agccacacac cacgccgcga gcgctgtcat cccaccatgg cgcgcccagg ccagtgagag
     6481 caggaacaaa ataaacaccc atagtcgaat ccactgaggc tggcagggta ttcagttcga
     6541 gggccagctc cgcgtctgtc agttcactta aaccggtgct gtcagccatc catgccacgg
     6601 catcgccggt gtgggggata tttccttcca gaccgtaaat aatattttcg ccatcatgcc
     6661 aggcaatggt tgtggcaagt gtggcgatat cacactgagc agagttgacg ggggccatga
     6721 cggaagaacc ggtcccatag gtcgctttca cacatcccgc ttctcccaac gcatgaccaa
     6781 aaagagcggc gtgagaatcg cccaccatcg ccatgacggg aataccatcc ggaattgttt
     6841 taagccctcg ggtataaccg aacaggccac tggaaggttt aatttctggc aatgcagcgc
     6901 gtggaatgtg aaacaacgcc agcatttcct catcccactc gcctgttttc aggttaagta
     6961 attgagttcg cgcggcgtta gagtagtcgc agcaaaatga atcgccagca gtcaagttcc
     7021 atagcaacca ggcatcaatg gtgccgagac aaatttcacc ctggtcggca agttgcacac
     7081 cctcaggtgt agattccagt agccaacgca tcttggacgc ggaaaatagc ggggcgatag
     7141 gaagacccgt agtcgtttta atttgctgct ctttgccctc gcgacgcagt gtttcacaaa
     7201 actcggcact gcgcgtgcat tgccaggtaa tcgccgcatt gagtggtttt ccactctgac
     7261 gataccagcc gatagccgtt tcacgctgat tactgatagc cagagccgct acattttctg
     7321 cccccacgtg gttgatggcc tgggcaataa catcaagcga ggcagagatt aacgcttccc
     7381 cggattgctc aacccagcct tcacgtggcg tctgaatagc cagtggttga gaaaacttcg
     7441 ccaccacgtt accgaaacca tcaagtgcga tggcttttgc attggtggta ccctcatcca
     7501 acgcaataac gaactcttta tttcctgcaa acataggtag tctcctgagt cacattgcat
     7561 cctggcgtgg aaggagatga ggcgttcctc atggtcacgt tattacttca tcaggccctg
     7621 ctgtgacatt ttgtgttaag gcatgaatat atattcgata ctgattattt atttatacct
     7681 tagccagatt attcttcctg agctagagaa aaaatcatca aatgtgctgg ctatcacact
     7741 tagaagcgtg gtgacagggt gagaaagcct cgagaaacag gattgttgaa tcctttcagt
     7801 ttattgatat taaaggcttt ttaagcaatc acctttgcga gaagattttt tcgcaaaggc
     7861 gggcagaaga aagggaggag aggattaatc cgcaatcagc attctggcgg tggggaaatc
     7921 agtgatcaaa acatcaatat aaccaccctg catggcgcct ttgatggcgg caactttgtc
     7981 tttgccgccc gccagtgcca ccacattcgg gcattttttg attttatcca gtgccatacc
     8041 aatcactgga tcttcatcgt cctgcaacac gggctgacca tgtttatcgt agtagtgcag
     8101 gcagatatcg cccacagcac cgcgatccgc tagtacctgt aacatatcct cgtgataata
     8161 gttgcccgag gtttttagca actgcgaggg ttcaaggata ccgatgccga caatggcgat
     8221 atcgacgtcg tcaaatctgg cgataacgtc agccacatcc tggcttgcca ccaggcgttt
     8281 tttctcttct accgaacctt caatgctttg agaaggaagc aaccaggctt cgcaattcag
     8341 atgctgggcc agggtctgtg tcaaaatagt tgcctgcaca ttgccgtttg gccccacgcc
     8401 gcccagcagc tgaataacgc cgctggcttt taagttttgg gcgtgaactt catcgaccat
     8461 cgcgcgaatc gtagaactcc acgaagagac gccaatgaaa tcttttggcc ttaaacgcgt
     8521 ttccagatag tgcgcggcgg cagagccgat ggcgcgttta atggtgtgat cactggcatc
     8581 atcttcggta tcgacaacaa tcgcttgttt aatgccgtaa cgctcttcaa tgcctttttc
     8641 cagattgaga aagatatttg acggctggac aacgctgatt ttaaccacgc cttctttctg
     8701 gcaacgggtt agcgctctgg atataaatga ctgagaaagc gagagaagtt gggcgatgtc
     8761 agactgtttt cgcccctcaa gatagtaaag agtggcgatc ttcaccagta gccgctgttc
     8821 atcctgctta gccatatatt tccccgtaaa tcattaacgt accgccctta tcttagaggc
     8881 ttttatcata cgcgaaaaaa gaagacagtt aagtgtgatg gagttctaac tttaaccaat
     8941 ccttaaaatc agcgatggac aaaaataaaa aacaggcggc ataattgaat aaagaatcat
     9001 tattgaatat atattcaaga tgatatcagg tcaactgatg attacccgga catgtaactg
     9061 acggtgtaag gaggcagaat gttcctgagt atgatgcagc agcgtaagtc ttgccgcgca
     9121 acgccctggg atttctggtt tttttagtcc ccggtggaat aaatattcca tgttgaaaat
     9181 aaattcagag gtattaaatg aatccctata gctatgatat ccagaccctt gagcgtaaag
     9241 cgcgggcggt acgccgccat atcgtgcgct taaatgccaa tagcccggca ggtggacaca
     9301 ccggggccga tctctctcag gttgaattac tgaccgcact ctattttcgt attctcaatg
     9361 tcgcaccgga tcgccttcac gacgatgacc gggatatata cattcagtca aaaggccatg
     9421 ctgttggatg ttactactgc gtgctggcgg aagcaggatt cttcccactg gaatggcttt
     9481 ccacttatca gcagccaaat tcacacttac ccggccatcc ggttcggcaa aaaacgccag
     9541 gtattgaact gaataccgga gcactggggc atggtttccc tgtggctgta ggacttgcgc
     9601 tggcggcgaa aaaaagtcaa agccgtcgtc gtatcttctt aattactggc gatggtgagc
     9661 tggctgaggg cagtaactgg gaagctgcac tggcggcggc gcattacggg cttgataacc
     9721 tggtcattat taatgacaaa aataatctgc aattagccgg gccaacgcgc gaaatcatga
     9781 acaccgatcc tctggcagag aaatggctgg cattcggtat gcaggttact gaatgtcagg
     9841 gcaatgatat ggcttcggtt gtcgcgacgc ttgaagagct taaacaggaa ggcaaaccga
     9901 atgtggtgat tgcccacacc actaaagggg caggcatttc ctttatacaa gggcgtgcgg
     9961 aatggcatca ccgggtgccg aaaggcgatg aaattgaaca ggcactggag gaactcaaag
    10021 atgcgtaaca gtgaacatct ggcaaatgtg atggttcagg cgtttatcga tgccgtaaat
    10081 gaggggatcg atttggtgcc tgtggtggct gattcaacgt ctacggcaaa aattgctccc
    10141 tttattaagc aattccctga ccgactggtg aatgtgggta ttgccgaaca gagcatggtt
    10201 ggtacagctg ccgggctggc gctgggcggc aaggtggctg ttacctgcaa tgccgcgccg
    10261 tttcttatcg ctcgcgctaa cgagcaaatc aaggttgatg tctgctacaa ctttaccaac
    10321 gtaaaattgt tcggtctgaa tgccggggcg agttatggac cgctggcgag cacgcatcat
    10381 gcgattgatg acattgcggt tatgcgtggt ttcggcaata ttcaaatctt tgccccgtct
    10441 tcaccgcggg agtgccgtca ggtgattgat tatgctcttc gctatcaggg gcctgtttat
    10501 atccgtatgg acggtaaagc cctgccggaa atccacgatg aggactaccg atttgtacca
    10561 ggtgccgtgg tgactttgcg cgagggcagc gaactggcgc tggtagccac aggctctacc
    10621 gttcatgaag tggtcgatgc cgcagaactt ttagcgcagt ctggtatcgc ggctacagtt
    10681 gtcagcgtac cgtcgattcg cccatgcgac accaaagcat tgctggcagc gttaaaagga
    10741 tgcaaagcag tgatgacggt tgaagagcat aacgtcaatg gcggccttgg tagcctggtc
    10801 gcggaagtgt tggcagaggc cggaacgggg atcacattaa aacgcgcagg cattatggat
    10861 ggagaatatg ccgcagcggc ggatcgtggg tggttgcgtc agcatcatgg atttgatgct
    10921 gccggaattg cagctcaggc acgggaaatg ctttgaatta cccgccagat aacccgcatg
    10981 ctgcagtgtg cgggtgttta tctgccaggc acaaggtaat aacattatta ccccttacta
    11041 accactgcac gatatggtcg ccattcggcc cgcttatatt catctgttga tgctgacaat
    11101 ttctgatttg gtagcgcaga aatcctgcat cagtttgtct ttcacatgcc ttaaatgcgc
    11161 tctctttggc ggaaaatgcc agtgtcagcg ccagagaaaa ggttaaaccg cattctgcga
    11221 gtcgctcgtg ttctgctggt gtaataatgt tgtctgtcaa ttctcttgcg gtttgtacag
    11281 agaaaatctc ttcgatatca atgccaatcg gttgacgaga taccacggct aatgccgtag
    11341 tcccacagtg actaatgctg ccgtataccc ccgtaggcca cacaggttgg cgtagctcgc
    11401 cgattgcagg cacacattta tagccatatt cccgcagcgc ataaattgca gcgatccgcc
    11461 cggctaaatg ctctgtttta cgtttacgtc cagcgtgttg aagttgtgcg tagtgcggca
    11521 gccagagtaa atcctgctcg cgaaaactcg ccggatcgaa ctcaacaaaa tgcagcgtat
    11581 gtccggcaaa ggggagggag gtatgcgtag ttttcatgtc aaccatatta gagaatgatg
    11641 acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacgtggtcc tgcaatatat
    11701 tgaattggca agatttttgt aggccggata aggcgttcac gccgcatccg gcatgaacta
    11761 cgcgcacttt gtcaacaatc tgacgttagc ttcagaagtg agtattaatg ctcatatacc
    11821 acgtacgtcc cggctcgtta taggtatacg ccccggcacc tgcgatatag ttggctcctg
    11881 ccagatcgcc cgtggtctgg gcattacccg cacgccacaa acgtttgtcg aacaggttgt
    11941 ccacgccgcc ggtcagactg acattcttgg tcacatccca ggtcgcgctc aggccaacaa
    12001 tgctgtacgg actgatttct ttggtttccg gtccaaccgc tggctgacct ttatagttgt
    12061 acttcttcgg ctgctgtttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc
    12121 gtgcctgcca gctcagcgtt gagttcaacg tatactccgg gatgatcgac aaacggtcgc
    12181 ccgtggtttt gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt
    12241 cgctaaccgg tacgtttaac gatccttcca gaccttcaac caccgctttc ggcacgttat
    12301 cccactgata gagatcggtg ccgactgcgt ttttccctac agctacatag cccgcttcaa
    12361 tcttattgcg ataatcgtta cggaaccagg tcacgcccgc cagccaaccg tcgcgtttga
    12421 actccagacc aatctccttg ttgatgctgg tttctgcttt caggtcatcg ttaccttgca
    12481 gatagcagcc gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagtttg
    12541 ggttagtctg gtacaggctc ggcgctttat aggcgcgggc gatgcccatt ttcagcgtga
    12601 agtcatcgcc taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac
    12661 tgtgatgatc gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatattgt
    12721 tttcggcaaa cagcgagaaa atttctgctt ttgaatatgg gctacggtcg gtggcactca
    12781 caccatcaat agcaccgccg gtattcgttc cggtgagtgc ctgggtgttg gaactcaagt
    12841 ccttcatccg ttgctgattc cactctgtac ccagcgtcag cgtttggttt acgaggaaat
    12901 caatcggcag gttaacttcg ctgtgcagca tcacgtcatc aagatcgata tcgacaaaat
    12961 cctgtgccgc tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac
    13021 gcgagttacg ggtgtgttcg tactgcaccc agttgctggt ggtcacgccg ttatcccagc
    13081 cgccgttcca ggtcagcgag tagttctggc gatacaggcg gttggtttca tcgccatatt
    13141 tcgagcgagt gtaagcgtca gagttggtgt tctgcgtatc acccgcatac aggttaccct
    13201 ggcggctgta gcccgcttcc agttcgagtg attgcagagg cgcgaagtcc cagcgcacca
    13261 cgccgttaat atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg
    13321 ttccggcacg cgcggactga tggccctggt tgatatccca cgcgtcagcc tgggttttgt
    13381 cgaggttgcc atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctgaagt
    13441 tggtgcgttt ggtggcacct tcctctttat gttctggcgc attgaaataa gcatcccagg
    13501 agccgtgcca ctcaccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc
    13561 cgttgccata acgcgcagct gccggaccac gtagaacttc aatacgttca atcatttcag
    13621 gcggcaccca ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcacag
    13681 agttacggct gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatgc
    13741 cgcgaatatc aatctggcgg ttattaccac gctgaccact ggtggaatta ccggtcaggt
    13801 taacgccagg catggtacga atgatctccg atacatcgcg ggcaaccggg tttttgcgga
    13861 tttcatctgc ggtaatggtc gaaacgccgg gcgcctgcaa gttctgctcg gcggcggtaa
    13921 caacaatagt atcgtcatgt gaaacaggag tatcggtcgg ctcttgtgcc tgtgctaccc
    13981 cataaatccc cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat
    14041 tcctgcattt ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc
    14101 ccgctaccgc gaaaaccttt gatcctgaaa gacacgcagt gcagctgatt aattaatgtc
    14161 cgcgcttccc acggcgcgcc attacgctat tgcaaatgca aatagttatc aataatatta
    14221 tcaatatatt tctgcaatca ataaaaaatt gcacagtaaa catggggtta tggtgtgacg
    14281 gcgttaaaag taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcaacgt
    14341 ctgaatgacg aaatgtttga agtcactttt tggtggcgtg atccacaagg ttctgaaggc
    14401 cactcggcga taaagcgcgt atgggtctat atcactggca tgaccgatca ccatcagaac
    14461 agccagcccc ggtcgatgca gcgaattgca ggaaccgatg tttggcagtg gacgacacaa
    14521 ctcaatgcca actggcgcgg cagctactgc tttattccta ccgaacgcga tgacattttt
    14581 tctgcaccat cccccgatcg cctcgaatta cgcgaaggct ggcgaaaact attaccccag
    14641 gcgatagccg atccgctgaa tccacaaagc tggaaaggcg ggcgagggca cgctgtttct
    14701 gcactcgaaa tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa
    14761 acgcctgcca aagaaattat ctggaaaagt gaacggttga aaaattcacg ccgtgtatgg
    14821 atttttacca tcggcgatgc aacagcagaa gaacgcccgc tggcagtttt gcttgatggc
    14881 gaattttggg cgcagagtat gcccgtctgg cctgcgctga cttcgctgac ccatcgtcgg
    14941 caacttccgc ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac
    15001 gaactgccgt gtaatgcgga tttctggctg gcagtacagc aagagttatt accccaggtg
    15061 aaagctattg ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg acagagtttt
    15121 ggtgggcttt ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta
    15181 agccagtcag gttcgtactg gtggccgcat cggggcgggc accaagaggg catgttactt
    15241 gaacagctaa aaactggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt
    15301 gttcgcgagc cgatgatcat gcaggccaat caggcgctgt atgcgcaatt acacccccta
    15361 aaagaatcca ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt
    15421 ggcttgatgc aggggctaat cgacctctgg caaccacttt tccatgacag gagttgaata
    15481 tggcattcag taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc
    15541 aggggcaatt cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacgttgtgt
    15601 gtcagccgca gtcacaggcg tcctgccagc agtggctgga agcccactgg cgtactctga
    15661 caccggcgaa tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc
    15721 cgcacagccc ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt
    15781 ggcgcattac gttgagttaa ccggagaggt tgatgcgcca ctactggccc gcgcggtggt
    15841 tgccggacta gcgcaagcag atacgttgcg gatgcgtttt acggaagata acggcgaagt
    15901 ctggcagtgg gtcgatgatg cgcagacgtt cgaactgcca gaaattatcg acctgcgaac
    15961 caacattgat ccgcacggta ctgcgcgggc attaatgcag gcggatttgc aacaagatct
    16021 gcgcgtcgat agtggtaaac cactggtctt tcatcagctg atacaggtgg cggataaccg
    16081 ctggtactgg tatcagcgtt atcaccattt gctggtcgat ggcttcagtt tcccggccat
    16141 tacacgccag atcgccaata tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc
    16201 gccgtttacg cctttcgctg atgtagtgga agagtatcag cagtaccgcg aaagtgaagc
    16261 ctggcagcgt gatgcggcat tctgggcgga acagcgtcgt caactgccac cgcccgcgtc
    16321 actttctccg gcacctttac cggggcgcag cgcttcggca gatattttgc gcctgaaact
    16381 ggaatttacc gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac
    16441 cgatttagca cttgcgttgg cagccttgtg gctggggcga ttgtgcaacc gcatggacta
    16501 cgctgccgga tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc
    16561 cgtgctcaac gttttaccgt tgggtattca cattgcggcg caagaaacgc tgccgcaact
    16621 ggcaacccga ctggcagcac aactgaaaaa aatgcgccgc catcaacgct acgacgccga
    16681 acaaattgtc cgtgacagtg ggcgagcggc aggagaggaa ccgctgttcg gtccggtact
    16741 caatatcaag gtatttgatt accaactgga tattcctggt gttcaggcgc aaacccatcc
    16801 cctggcaacc ggtccggtta atgaccttga actggccctg ttcccggatg aacacggaga
    16861 tttgagtatt gaaatcctcg ccaataaaca gcgttacgat gagccaacgt taattcaaca
    16921 cgctgaacgc ctgaaaatgc tgattgccca gttcgccgcg gatccggcgc tgttgtgcgg
    16981 cgatgtcgat attatgctgc caggtgagta tgcgcagctg gcgcagatca acgccactca
    17041 gattgagatt ccagaaacca cgctgagcgc gctggtggca gaacaagcgg caaaaacacc
    17101 ggatgctccg gcgctggcag atgcgcgtta ccagttcagc tatcgggaaa tgcgcgagca
    17161 ggtggtggcg ctggcgaatc tgctgcgtga gcgcggcgtt aaaccgggcg acagcgtggc
    17221 ggtggctcta ccgcgctcgg tctttttgac tctcgcgcta catgcaattg ttgaggctgg
    17281 tgcggcctgg ttaccgctgg ataccggcta tccggacgat cgcctgaaaa tgatgctgga
    17341 agatgcgcgt ccgtcgctgt taatcaccac tgacgatcaa ctgccgcgct ttgccgatgt
    17401 tccagattta acccgccttt gctataacgc cccgcttaca ccgcagggca gtgcgccgct
    17461 gcagctttca caaccgcacc acacggctta tatcatcttt acctctggct ccaccggcag
    17521 gccgaaaggg gtaatggtcg ggcagacggc tatcgttaac cgcctgcttt ggatgcaaaa
    17581 ccactacccg cttacaggcg aagatgtcgt tgcccaaaaa acgccgtgca gttttgatgt
    17641 ctcggtgtgg gagtttttct ggccgtttat tgccggggct aaactggtga tggctgaacc
    17701 ggaagcgcac cgcgacccgc tcgctatgca gcaattcttt gccgaatatg gtgtaacgac
    17761 cacgcacttt gtgccgtcga tgctggcagc atttgttgca tcgctgacgc cgcaaaccgc
    17821 tcgtcagaat tgctcgacgt tgaaacaggt tttctgtagt ggtgaagccc taccggctga
    17881 tttatgccgc gaatggcaac agttaaccgg cgcgccgttg cataatctat atggcccgac
    17941 ggaagcggcg gtagatgtca gttggtatcc ggcttttggc gaggaactgg cagaggtgcg
    18001 cggcagcagt gtgccgattg gttatccggt gtggaatacg ggcctgcgca ttctcgatgc
    18061 gatgatgcat ccggtgccgc cgggtgtggc gggcgatctc tatctcaccg gtattcaact
    18121 ggcgcagggg tatcttggac gacccgatct gaccgccagc cgctttattg ccgatccttt
    18181 tgccccaggt gaacggatgt accgtaccgg agacgttgcc cgctggctgg ataacggcgc
    18241 agtggagtac ctcgggcgca gtgacgatca gctaaaaatt cgcgggcagc gtatcgaact
    18301 gggcgaaatt gatcgcgtga tgcaggcgct gccggatgtc gaacaagccg ttacccacgc
    18361 ctgtgtgatt aaccaggcgg cagccactgg tggtgatgcg cgtcagttgg tgggctatct
    18421 ggtgtcgcaa tcaggtctgc cgttggatac cagcgcatta caggcgcagc ttcgagaaac
    18481 attgccacca catatggtac cggtggttct gctgcaactt ccacagttac cacttagcgc
    18541 caacggcaag ctggatcgca aagccttacc gttgcctgaa ctgaaggcac aaacgccggg
    18601 gcgtgtgccg aaagcgggca gtgaaacgat tatcgccgcg gcattctcgt cgttgctggg
    18661 ttgtgacgtg caggatgccg atgctgattt ctttgcgctt ggcggtcatt cgctactggc
    18721 aatgaaactg gcagcgcagt taagtcgaca gtttgcccgt caggtgacgc cggggcaggt
    18781 gatggtcgcg tcaactgtcg ccaaactggc aacgattatt gatggtgaag aagacagcac
    18841 ccgacgcatg ggattcgaaa ccattctgcc gttgcgtgaa ggtaatggcc cgacgctgtt
    18901 ttgtttccat ccggcatccg gttttgcctg gcagttcagc gtgctctcgc gttatctcga
    18961 tccacaatgg tcgattatcg gcattcagtc gccgcgccct catggcccca tgcagacggc
    19021 ggcaaacctg gatgaagtct gcgaagcgca tctggcaacg ttatttgaac aacaaccgca
    19081 cggcccttat tacctgctgg gttattcact tggcggtacg ctggcgcagg gcattgcggc
    19141 gcgactgcgt gcccgtggcg aacaggtggc atttcttggc ttgctggata cctggccgcc
    19201 agaaacgcaa aactggcagg aaaaagaagc caatggtctg gacccggaag tgctggcgga
    19261 gattaaccgc gagcgcgaag ccttcctggc ggcacagcag ggaagtactt caacggagtt
    19321 gtttaccacc attgaaggca actacgctga tgctgtgcgc ctgctgacga cagctcatag
    19381 cgtagcgttt gatggtaaag cgacgctatt tgttgccgaa cgcacgcttc aggaaggtat
    19441 gagtcctgaa caggcctggg caccgtggat tgctgagctg gatatctatc gtcaggattg
    19501 tgcgcatgtg gatattattt ccccggagta ttttaaagaa ataggaccac taataaatac
    19561 acaaattaat aactaaccgt acgttagtaa agaacttgcc ataacctcat ttaaaaccat
    19621 aatccgacta aatgattatg gtttttttat tcttaaaatt tatttatttt tctgagagca
    19681 gcgtcattgc tggggttgct aagtttgtta ttcattttga gaataataat agtgaaagag
    19741 tgctttcgga taaatctcag ttcctgttag aatgctatgt acatcgttgt gctttacgga
    19801 ctaaaacctc aggtttcact acatgtcatc actgaatatt aaacagggaa gtgaagctca
    19861 ttttcccgag tatcctctgg cgtcgcccag taataatgaa attgatttac ttaatctaat
    19921 cgaggtttta tggcgggcaa aaaaaacggt catggcggtc gtttttgcgt ttgcctgcgc
    19981 aggcttgctg atctctttca tcctgccgca aaaatggact agcgcggcgg ttgtcacgcc
    20041 tccagaacct gttcagtggc aagagctgga gaaaacattc actaagcttc gtgtgctgga
    20101 tctggatatc aaaattgatc gtacagaagc atttaacctg tttatcaaga aatttcagtc
    20161 tgttagcttg cttgaggagt acctgcgttc atcaccttat gtgatggatc aattaaaaga
    20221 agcgaaaatc gacccactgg atctgcatcg cgccattgtt gcattgagcg aaaaaatgaa
    20281 agcggttgat gacaatgcca gtaagaaaaa agatgagtct gcattgtata cttcctggac
    20341 gctaagtttc accgcaccca ccagtgaaga ggcacagaaa gtgttggccg ggtatatcga
    20401 ttatatctct gcgttggtgg tgaaagagt
//
LOCUS       DAOFSV010000076        20319 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.076, whole genome shotgun sequence.
ACCESSION   DAOFSV010000076 DAOFSV010000000
VERSION     DAOFSV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.076"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(129..455)
                     /locus_tag="QBH27_002868"
     CDS             complement(129..455)
                     /locus_tag="QBH27_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDQ7015160.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(463..648)
                     /locus_tag="QBH27_002869"
     CDS             complement(463..648)
                     /locus_tag="QBH27_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDQ7015161.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(645..3284)
                     /locus_tag="QBH27_002870"
     CDS             complement(645..3284)
                     /locus_tag="QBH27_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDQ7015162.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLAPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            3492..4481
                     /gene="ldhA"
                     /locus_tag="QBH27_002871"
     CDS             3492..4481
                     /gene="ldhA"
                     /locus_tag="QBH27_002871"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDQ7015163.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4592..5014
                     /gene="hslJ"
                     /locus_tag="QBH27_002872"
     CDS             4592..5014
                     /gene="hslJ"
                     /locus_tag="QBH27_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDQ7015164.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5011..5277)
                     /locus_tag="QBH27_002873"
     CDS             complement(5011..5277)
                     /locus_tag="QBH27_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDQ7015165.1"
                     /translation="MRAAFWVGCAALLLSACSREPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5551..9075
                     /gene="nifJ"
                     /locus_tag="QBH27_002874"
     CDS             5551..9075
                     /gene="nifJ"
                     /locus_tag="QBH27_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDQ7015166.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9441..10574
                     /gene="ompN"
                     /locus_tag="QBH27_002875"
     CDS             9441..10574
                     /gene="ompN"
                     /locus_tag="QBH27_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDQ7015167.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10715..11149
                     /gene="uspF"
                     /locus_tag="QBH27_002876"
     CDS             10715..11149
                     /gene="uspF"
                     /locus_tag="QBH27_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDQ7015168.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11238..11838
                     /locus_tag="QBH27_002877"
                     /pseudo
     CDS             11238..11838
                     /locus_tag="QBH27_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(13026..13151)
                     /locus_tag="QBH27_002878"
                     /pseudo
     CDS             complement(13026..13151)
                     /locus_tag="QBH27_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            13193..13423
                     /locus_tag="QBH27_002879"
                     /pseudo
     CDS             13193..13423
                     /locus_tag="QBH27_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            13475..14410
                     /gene="ttcA"
                     /locus_tag="QBH27_002880"
     CDS             13475..14410
                     /gene="ttcA"
                     /locus_tag="QBH27_002880"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDQ7015169.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14538..15911)
                     /gene="dbpA"
                     /locus_tag="QBH27_002881"
     CDS             complement(14538..15911)
                     /gene="dbpA"
                     /locus_tag="QBH27_002881"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDQ7015170.1"
                     /translation="MTAFSTLNVLPPAQLTNLNGLGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(16389..17372)
                     /gene="zntB"
                     /locus_tag="QBH27_002882"
     CDS             complement(16389..17372)
                     /gene="zntB"
                     /locus_tag="QBH27_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDQ7015171.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            17627..18859
                     /gene="dgcM"
                     /locus_tag="QBH27_002883"
     CDS             17627..18859
                     /gene="dgcM"
                     /locus_tag="QBH27_002883"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDQ7015172.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSTNYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(18880..19443)
                     /gene="smrA"
                     /locus_tag="QBH27_002884"
     CDS             complement(18880..19443)
                     /gene="smrA"
                     /locus_tag="QBH27_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDQ7015173.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSRPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(19638..>20319)
                     /locus_tag="QBH27_002885"
     CDS             complement(19638..>20319)
                     /locus_tag="QBH27_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015174.1"
                     /translation="IFSTVYLIPVFLGRVRGLNAEEIGFAVCTTGIFQLFSVPFYFWL
                     SKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLT
                     LGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVN
                     DFISRSALFFNRGGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLL
                     VPAMNKSS"
BASE COUNT         5384 a         5089 c         4666 g         5180 t
ORIGIN      
        1 gatatttgac tatgcatttt tcagggcgaa aggcaatttg acaggcgttt ttgaataatc
       61 atcttgtttc cggaaaatag attaatcgcc ttctcctgta gcgttatccc gatattgcgg
      121 ctttactatt aaaattttcg cacccattta ccgtttatcc cttgtacata ttccccgggt
      181 ttggcccgtt ctacgagctt ttgcccggcg agtttcgcga tatcatctac cgacacatta
      241 ttttgctttg ccagttgttg atagctcgct ttacgttcgg cattaatgtc ggttactaat
      301 ttctcagttt cagcatctgt tttcagcgca accagataac cataaaatgt ttcgcctacc
      361 cgcccctgag ttctggcttc atccagagtc aatgccatta cattgctgct taccaagcca
      421 acaagaaatg cacaaagaag taatgttctt ttcatcatca cctcaaaaaa gatcgctacg
      481 ggtctcaagc agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt
      541 aacgttcata ttgatagtga tcggttcctt aggtgcagcg acttcaatac gaggcgtaca
      601 gccaaccagc ataaaagatg acgtcaacgc agccagtaaa attttcattg tttatcctca
      661 cactcttttc cttgcggaca gtcatttccc ggcagacgcg cgttctgctc cagccatgcc
      721 tggagattat cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg
      781 taattaagat ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatattggcc
      841 tgcatggtta ataaaccgag attatctaaa ttaatttttg tcgatgaacg gctaatttcc
      901 atatagcgca accagttaat tgctgaaccc gcggtcatat tgtctttcac caccgcatct
      961 gcggtgtctt tatccagccg caaggtcatt ggtccgctat ttgccagcca gccgtctttc
     1021 actatccatt tttcattgtt taaccataac ggcagtgcac cactaaatgc cccggacatg
     1081 gcgaattgtt tcggattaac ggcgctaacc agttcgctgg atgagagatt attcagtcgc
     1141 aacagtgccg ggtcatgttg cggcatacgt aattgtttca tcagcacgtt accgcctaac
     1201 acatcgacac taacatcagt caacagcaat ggttcttctt cagtccacgg ataacgccct
     1261 tgcaaatcag ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac
     1321 gtaacggggc cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca
     1381 aaatcgacac cgttaacctg attatctggc atccaggcac taccgccttt caatacgccg
     1441 tgcccccccg cgcggaatcc ttgttcgggc gctgctgaaa atgcgacctg agcatacagt
     1501 tcaccatcgc gtaagttcat cttccagtcg ggtggcgcca acggctggaa tacagtcagt
     1561 gattgtttag gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc
     1621 cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg
     1681 cgcccatcga cactaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc
     1741 gtttgcccgg cgtccagtga cagagcgccg ctgaaggagg gatgctgtgc gtcacgtccc
     1801 cagacgacgg gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt
     1861 aactggtcga aaccggtgga gagatcggtc agcgtaatcg tgctgtcatg ccactcacct
     1921 tttcctgcga catcccaggt ggcattcatc ggtgtaaaac tcccttttcc ccagtagcgc
     1981 cactgccagc ggccagcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg
     2041 aaatcgccca gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca
     2101 cgttgggtga cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatca
     2161 atgactcttc cctttgaacg aagtaacgcg ccaggctcaa aggtcagcgt tgggtcagac
     2221 agacttccac ttagctgcgc aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct
     2281 tcaccggtca gctgcagagg cagctggctg ttatccatgc ttaattttcc tgggccaaaa
     2341 tttaacaccg cgttgccttt acccgcttgc ccctgggtca gcacactcag tcgcccgctg
     2401 accagagcgt tctcgagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc
     2461 aaaggaaaac ctgcatacgg ccagctccag cgaccatcgc ttacggtcaa ttgttgacga
     2521 gtaatttgcc acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacaatcaat
     2581 tgcccgctat tttcctgcca gtccagctcg gcatccacca gtgaaggctc ctgcggtaag
     2641 tttaacgtcg cagtagcatg accacttaca ggaagtccat ccggcacgag cggcatagta
     2701 aattccccca ccagttttac cggcggctga ttttcaaacg cgacgacatc cagttcgctg
     2761 actgtaagtt gttgcccttt aagctggcct tgaaatttaa ctttttcgcc ctgataacgc
     2821 agttgctgga tatcggaggt taatgcgaga gagagttttc cctgccattc ctgccaggga
     2881 gaaaaaatca gtttatcgat attgatccag gtgttaggca acatggcctg ccactgcgcg
     2941 agggttttgg gagcggctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa
     3001 tcaagttcta ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg
     3061 gtgatatgcg caagctgaca atctcccacc agataacgga gatcggggat gattaaacct
     3121 ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg
     3181 cccgccagcg tgggaaccca cagcccgagc gtcatcagca gcgtcaacgg cacaagaata
     3241 atcagtaata acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct
     3301 gatttagcga aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg
     3361 aaggttgcac ctacactaag catagttgtt gatgaatttt tcaatatcgc cataactttc
     3421 aattatattt gaaattttgt aaaatatttt tagtagctta aatgtgattc aacatcactg
     3481 gagaaagtct tatgaaactc gccgtttata gcacaaaaca gtacgacaag aagtacctgc
     3541 aacaggtgaa cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa
     3601 aaaccgctaa aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca
     3661 gccgcccggt gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg
     3721 ccggtttcaa taacgtcgac cttgacgcgg caaaagaact ggggctcaaa gtagtccgtg
     3781 ttccagccta tgatccagag gccgtagctg aacacgccat cggtatgatg atgacgctga
     3841 accgccgtat tcaccgcgcg tatcagcgta cccgtgacgc taacttctct ctggaaggtc
     3901 tgaccggctt tactatgtat ggcaaaacgg caggcgtgat cggtaccggt aaaatcggtg
     3961 tggcgatgct gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc
     4021 caagtgcggc ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg
     4081 aatcagacgt tatctctctg cactgcccgc tgacaccgga aaactaccat ctgttgaacg
     4141 aagccgcctt cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat
     4201 tgattgattc tcaggcggca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta
     4261 tggacgtgta tgagaacgaa cgcgatctgt tctttgaaga taaatccaac gacgtgatcc
     4321 aggatgacgt attccgtcgc ttgtctgcct gccacaacgt gctgtttacc gggcaccagg
     4381 cattcctgac agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca
     4441 atctggaaaa aggcgaaacc tgcccgaacg aactggttta atcttgccgc tcccctgcat
     4501 tccaggggag cttattcaga taatccccaa agacctttca tcctctattc ttaaaatagt
     4561 cctgaatcag aaacagtaat tgagaaccac aatgaagaaa gtagccgcgc ttgttgcgct
     4621 aagcctgctg atggcgggat gtgtaagtag tgacaaaatt gctgtaacgc cagaacagct
     4681 acagcatcat cgctttgtgc tggaaagcgt aaacggtaag cccgtgacca gcgataaaaa
     4741 tccgccagaa atcagctttg gtgaaaagat gatgatttcc ggcagcatgt gtaaccgctt
     4801 tagcggtgaa ggcaaactgt ctaatggtga actgaccgcc aaagggctgg caatgacccg
     4861 tatgatgtgc gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa
     4921 agaaggtgca caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac
     4981 attaacttat aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag
     5041 tgactgttcg ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc
     5101 cagttgacgg gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact
     5161 ggacatcgac gcttttaaac ctggcgctac gtgcgccgca gtcgcctgct gaacaggttc
     5221 acgactacac gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctt
     5281 ttcttcctct gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc
     5341 ggcgtcgaga gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt
     5401 gccctctgtt tgatcaaaac attcattacg ctgatgtggg ggacacaaaa gcgaaaatgc
     5461 agaagaaagc catttgctaa aattgaacga ttactactgg gcgcgcagca atttcgtgcg
     5521 cccctcattt gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt
     5581 gcttcggtgg cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc
     5641 acgatggcag aacaggctga tgcatgggcc ggaaacggct tgaaaaacgt ttggggagac
     5701 acaccacgcg tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggtgct
     5761 ttgcaaactg gtgccctctc aacatcgttt acgtcatcgc agggtttgct gctgatgatc
     5821 ccaacgctgt acaaactggc aggcgaactc acaccgtttg tcctgcatgt tgcggcacgt
     5881 accgttgcca cacatgcact ctctattttt ggcgatcatt ccgacgttat ggcagtgcgc
     5941 caaacgggtt gcgcgatgtt gtgtgcagca aacgtccagg aagcgcaaga ctttgctctc
     6001 atttcgcaca ttgcgacgct gaaaagtcgc gtgccattta ttcatttctt tgatggtttc
     6061 cgcacatctc acgaaatcaa taaaattgtc ccgctggccg atgacacgat tcttgaactg
     6121 atgccgcagg cagaaattga tgctcatcgc gcccgggcac tcaacccgga acatccggtg
     6181 atccgcggta cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaaccca
     6241 tggtacaacg cggtctatga ccatgttgaa caggcgatga atgatttcgc tgccgcgaca
     6301 ggtcgtcagt atcagccgtt tgagtattac gggcatccgc aagcggaacg ggtgattatc
     6361 ctgatgggct ctgccattgg caccagtgaa gaagtggttg atgaattgct gacccgtggc
     6421 gaaaaagtcg gcgtgctgaa agttcgcctg taccgcccct tctccgccaa acacttgctg
     6481 caagctctgc cgggatccgt gcgcaacgtg gcggtactgg acagaaccaa agaacccggt
     6541 gcccaggcag aaccgctcta tctggatgtg atgactgcac tggcagaagc ctttaataat
     6601 ggcgagcgcg aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa
     6661 tttggcccgg actgtgtact ggcggtattt gccgagctca acgcggctaa accgaaagcg
     6721 cgctttacgg ttggtattta cgatgatgta accaatctgt cactgccgtt gccggaaaac
     6781 accctaccaa actcggcgaa actggaagcc ttgttttatg gccttggtag tgatggcagc
     6841 gtttccgcga ccaaaaacaa tatcaagatt atcggaaatt ccacgccgtg gtacgcacag
     6901 ggctattttg tttacgactc caaaaaggcg ggcggcctga cggtttctca ccttcgcgtg
     6961 agcgaacagc cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac
     7021 cagttgcagt ttatcgataa atatcagatg gctgagcgtt taaaacctgg cggcattttc
     7081 ctgctcaaca cgccgtacag cgcagctgaa gtgtggtcgc gcttgccgca ggaagttcag
     7141 gccgtgctaa accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc
     7201 gaatgtggcc tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg
     7261 cagattctgc ctggcgatag tgccctcgca gaattgcagg gtgcgattgc caaaagttac
     7321 agtagcaaag gccaggatct ggtggaacgc aactggcagg ctctggcgct ggcacgtgaa
     7381 tccgtagaag aagttccact gcagccagtt aatccgcata gcgccaatcg accgccggtg
     7441 gtttccgatg ccgctcctga tttcgtgaaa accgtaaccg ctgcgatgct cgccgggctt
     7501 ggcgacgccc tccccgtttc ggcgctgccg ccagacggca cctggccgat gggcaccacg
     7561 cgctgggaaa aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc
     7621 caatgtaacc actgtgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtaccg
     7681 cctgaagcga tggaaaacgc ccctgccagc ctgcattcgc tggatgtgaa atcgcgtgat
     7741 atgcgcgggc agaaatatgt cttgcaggtg gctccggaag attgcaccgg ttgtaacctg
     7801 tgcgtcgaag tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg
     7861 atgtcgcgcc tggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg
     7921 ccagaaatcg atcgtagcaa actggaacgt attgatattc gtacatcgca gctaatcacg
     7981 ccgctgtttg aatattccgg tgcttgctcc ggttgtggcg agacgccgta tattaaattg
     8041 ctgactcagc tctatggcga ccggatgttg atcgctaatg ccactggctg ttcttcaata
     8101 tacggcggta acctgccttc aacaccatat accaccgatg ccaacggtcg tggaccggca
     8161 tgggcgaact cgctgtttga agataacgcc gaatttggcc ttggtttccg cctgacggtc
     8221 gatcaacacc gtgtccgcgt gctgcgtctg ctcgatcaat ttgccgataa aatcccgacg
     8281 gaattactga ctgcgttgaa atcagacgcc acgccagagg ttcgtcgtga acaggtagca
     8341 gctttacgcc agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat
     8401 gcactggtgg aaaaatcaat ctggctgatt ggtggcgatg gctgggctta cgatatcggc
     8461 tttggcggtc tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat
     8521 acgcaatgct attccaacac cggaggtcag gcatcgaaag cgacaccgct gggtgcagta
     8581 actaagtttg gcgagcatgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg
     8641 atgtacggtc atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg
     8701 aaagcgattc aggaagcgga agcgtatccg gggccatcgc tgatcatcgc ttatagcccg
     8761 tgtgaagagc atggttacga tctggcactc agccacgatc agatgcgcca actcactgct
     8821 accggcttct ggccgttata tcgcttcgat cctcgtcgtg ccgatgaagg caaactgccg
     8881 ctggcgctgg attcacgccc gccgtcagaa gcacttgaag aaacgttact tcacgagcag
     8941 cgtttccgtc gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct
     9001 gcagctgatt tgcaaaaacg ctatgacttc ctggcacaaa tggccggaaa agcggaaaaa
     9061 agcaacaccg attaaatgct ctggataagg attatccaat tcaaaaaaaa agcccggacg
     9121 actgttcggg cttgtcttct tatgtgttgg aaaatcagta gcaatgcaat ggcccaacag
     9181 aaaataaaat ctgtgacaat aaaggcatat aacccgcgca gaataacgta tagaaaatta
     9241 aaagtataat ttttattttg tatgtgttat ctccaataca ttcaccagac ttattatcat
     9301 ttcagaagaa ttatttaatt aaggttttac ttaaggcgta acaaatgata tttatcggct
     9361 aactgaactt ctcctttagg atgttttcac tccccgaaat ggggatgaaa ggcaaataaa
     9421 ataactaaag gatttattca atgaaaagca aagtactggc actcttaatt cctgccctgc
     9481 tcgctgcagg tgctgcacat gcagccgaag tttataataa agacggcaac aaattggatc
     9541 tgtatggcaa agttgatggc ctgcattatt tttctgataa ttcagcgaaa gatggcgacc
     9601 agagctatgc tcgtctgggt tttaaaggcg aaacccaaat taacgatcaa ctcactggct
     9661 acggtcaatg ggaatacaat attcaggcaa acaacaccga atcttcaaca aaccagtcat
     9721 ggacccgtct ggcatttgcc ggactgaaat ttgcagatta cggttctttc gattacggac
     9781 gtaattatgg cgtaatgtac gacatcgaag gctggactga tatgctacct gaatttggcg
     9841 gtgactctta taccaatgca gacaacttta tgactggtcg tgccaatggc gtcgcgactt
     9901 atcgtaatac tgatttcttc ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag
     9961 gtaacaacga aggtgccagt aatggtcagg aaggcaccaa caacggacgt gatgttcgcc
    10021 atgaaaacgg tgacggctgg ggtctttcca caacatatga tttaggcatg ggctttagcg
    10081 ctggtgcggc atacacctct tctgaccgca ccaatgacca ggttaaccat actgcggcgg
    10141 gtggtgataa agcagacgcg tggactgctg ggctaaaata cgatgctaac aatatttacc
    10201 tggcaaccat gtattcagaa acgcgtaata tgaccccgtt tggcgacagc gattatgctg
    10261 ttgcaaacaa aacccagaac tttgaagtca ctgcacagta ccagtttgat tttggtctgc
    10321 gtccggcagt ctctttcctg atgtctaaag gccgtgacct gcacgctgcc ggtggtgcag
    10381 acaatccggc aggtgttgat gataaagatc tggttaaata cgccgatgtt ggcgcgactt
    10441 actatttcaa taaaaacatg tccacctatg ttgactataa aatcaacctg ttggatgaag
    10501 atgacagttt ctacgctgcc aacggcatct ctaccgatga tattgtcgct ttaggtctgg
    10561 tttatcagtt ctaaatcccc ctgcccgctg ttttggcggg ctttttctgc ttattcttcc
    10621 tcctttgatc taaattaaaa atgtgaactc cgtcattaca caaaaagtgt catctggcgt
    10681 tacactttat gcggatacta aaacaggagg ttttatgaac agaacgattc ttgtccctat
    10741 cgatatttcc gattcagaat taactcaacg cgtgattagc cacgttgagg cagaggcaaa
    10801 gattgatgat gcagaggttc atttcctgac ggtaatacct tcgctgccct actatgcctc
    10861 tctgggttta gcgtattccg cagaattacc ggcaatggat gacctgaaag cggaagccaa
    10921 atcgcaactg gaagagatca ttaaaaaatt taaactgcca accgacagag tgcatgtcca
    10981 tgttgaggaa ggctcgccca aagaccgcat tctggaattg gcgaagaaga tccccgctca
    11041 tatgatcatc attgcttccc atcgaccgga tatcaccact tatctgctcg gttctaacgc
    11101 cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac actaacgccc
    11161 gcacattgct gcgggctttt tgattcattt cgcaaatgtg ctgatatttt cccctctaat
    11221 cattacaatt taaatttatg aattatgcat tgataatcgc tattataata tcgttcccct
    11281 gtgcattatt atcagttttt ctggtattaa aaggctgggc gctaatagga gatgcaatga
    11341 gccacgcaat gtttcctggc atagttctag tctggattat aggtatgccg ctgggaattg
    11401 gtgcatttat tgtaggactg tcttgtgcaa ttgcaactgg ctatctgaaa gataatagca
    11461 ttataaagaa agataccatt ataggtatcg ttttttcagg aatttttgtc ttgactatca
    11521 acaggcacaa gtcagcggat tacagatatc attattacat tacggtttac tctgcatggt
    11581 atcgcctacc atttttgcca cattaaaagc agtcggaatt attctgtcta tatactatta
    11641 atagctccag ggtctattgc tgtcctcatc atgcatcgct ttcataaggc attacttatc
    11701 gccgtaacga tatcaataac aatatcaggc gtatggttat tatattttct caacagagcc
    11761 ccagcgccga caatcgtcct cttatttgct gtagcatttc ttttcgcgct gataataatc
    11821 aattttcgga aacaataatc tcaacagtag aataaagagg caaatcaatc attacatata
    11881 taagatggtg ctaatattgt accgccttta aattgaactt tatcctttca cccttacaca
    11941 atttaaggaa tgcagatgac tattttatca gcccccagcc tttttcctgg taatcgaaaa
    12001 cttgaaaatc attaactcta agaagttctg ccaaatcgtt gaaattactt taaattagac
    12061 tcgaacaaat caatccctga tggggctttt acttatccat ttaatcgggt agatatcgac
    12121 ctccatctgc ccgcctcaga ttcgtacctg gtggaggtct tttttcgtta cctctttccc
    12181 cctttagttc agtataaaat catcaaacta atgctgttca cactatttac ctccagcatt
    12241 ctatataaat aatagaaata aaaaccccaa tacatagtgc attggcatga ccaaacatca
    12301 atgccattat actcgcctct gttaaatact cgctagttaa tatgataggc acgcatgata
    12361 taacaatcca tatgctaata cataaataca tcagtatttt tctcatagtc agtccctgtg
    12421 attaacaatc aaggcatagt taagcatatt tcacattcaa acgtaaacat tattaataac
    12481 attatatcaa tcgaactgac atcacgattc aagccctctt caaactgaag gttagagcag
    12541 cgtagaacat ttcaaaacaa tacactaaaa aaatacctga acaaaaaact taattctttt
    12601 ttattgtgaa ctttagtgat tttgtcatgc aactctttat tacatttagg cctgtattat
    12661 ttcatggcaa cgcagataca ggaccatttc ttgtgctatg gataagtaat aagctagtta
    12721 catcttatta agagaaccat tttcacatat caagatatca tcaccaccca caatgaatta
    12781 agtatttttc ttattcttta gattagatac gtaatgaatc cctaactcca ttcgggtgtc
    12841 atggctcttt catttgaaat atattcatat ccaatgtaat atttccatgc gcaaatataa
    12901 tcaccaacca tacccatcat ttattacaga gaattatttt attcaccgaa aggtttcaca
    12961 aaatatcccc tgatagaaaa ctaaaaaaca ctgcataata tcgctattct ggcacttgcg
    13021 aaaaattata ttctgcacct gttgtagcgt tctatttctc tgcctggcaa cgaatgcaca
    13081 atgtccgcca cagtttctgt tctaatttct tcctgtgtta ccgcatcctg tacgggtagc
    13141 gcaacaccag catgctaagc aaggctttcc catcagattt cactacgact atttgggagc
    13201 gagatggaat gcagcagtaa aacgtgctgg tattcgccgc cgtaatccgt accatacgcg
    13261 acatactttt gcctgctggc tgttgacggc aggagcgaac ccggcattta tagccagcca
    13321 gatggggcat gaaactgcgc agatggtgta tgaaatttac ggtatgtgga ttgatgacat
    13381 gaacgacgaa caggtagcca tgttgaatgc gcggttatcg tagttgcaaa gtttgccctc
    13441 aatatgcccc atttagtacc agagaactga aataatgcaa gaaaatcaac aaattacaaa
    13501 gaaagaacaa tacaacctga acaaattaca aaaacgtctg cgtcgtaacg tgggcgaagc
    13561 cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc tctccggggg
    13621 taaagacagc tataccatgc tggagattct gcgcaatttg cagcaaagcg cgccaatcaa
    13681 tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg aacacgttct
    13741 gcccgagtat cttgaaacgc tgggcgttga gtacaagatt gttgaagaga acacttacgg
    13801 tatcgtgaaa gagaagattc cggagggcaa aaccacttgc tcactgtgtt ctcgtcttcg
    13861 tcgcggtatc ctttatcgca ccgcaacgga actgggggcg acgaagatcg cgctgggtca
    13921 ccatcgtgac gatatcctgc aaacgctgtt cttaaatatg ttctacggtg gcaagatgaa
    13981 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgtgattc gtccgctggc
    14041 ctactgccgc gagaaagata ttcagcgctt tgccgatgca aaagcgttcc cgattattcc
    14101 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtaattgctg acatgttgcg
    14161 tgactgggat aaacgttatc cggggcgtat cgagacgatg ttcagcgcaa tgcagaatgt
    14221 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatta cccacggttc
    14281 tgaagtggtt aacggggggg atctggcgtt tgatcgcgaa gagatcccac tacaaccagc
    14341 gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
    14401 agtgaaataa ccaggatagc gcccgatacg caagcgtatc gggctactct tatggaagcc
    14461 gataagacgc ggccagcgtc gcatccggca atgccgaaga agatgtttac tcttgcaccc
    14521 tgcaattcaa catttcatta ttttaataac cgcacccggc acgtttttcc tttaatcttc
    14581 ccgccctgta actgtttcca tgctttatga gcaacagcct gacgcaccgc gacataaaca
    14641 tgcgccggat gcacggcaat tttgccaata tctgcgccat caagcccgat gtctcctgtc
    14701 agcgcaccta atacatcacc cgggcgcatt ttggcttttt tcccgccatc gatacacaac
    14761 gttgccattt ctgcttccag cggcacaatg gaactattcg ctggcggcgt ttgccagtta
    14821 agttttatct gcaacatgtc agaaatgata ttggcccgct gtgcttcttc cggagcacag
    14881 aaactgatcg ccagaccgct atttcctgca cgagctgtac gaccgatgcg atgtacatga
    14941 acttcagggt cccacgccag ctcaaagttc accaccagct caagcgattt tatatccaga
    15001 ccacgcgcag caacatcagt cgcgaccagt acacgggcgc taccgttagc aaaacgtacc
    15061 agggtctgat cgcgatcgcg ttgctccaga tcgccgtgta acgacaatgc actttgccct
    15121 acttcattca gcgcgtcgca gacagactgg caatcttttt tggtattgca aaacaccacg
    15181 caagaggatg gctgatgcaa gcttaataac cgttgcaaca gaggaatttt gcctttgctg
    15241 gatgtctcat aaaattgttg ttcaataggt ggcagagcat ctgttgagtc aatttcaatc
    15301 gctaaaggat cgcgctgcac tcttccgctg attgcggcaa tggcttccgg ccaggttgcc
    15361 gaaaacagaa gtgtctgccg agatgctggc gcaaaacgga tgacatcatc aatggcgtca
    15421 ctaaatccca tatccagcat gcggtcggcc tcatccatca ccagcgtatt caacgcatcc
    15481 agtgatacag tgcctttttg caggtgatcc agcaaacggc ccggcgttgc cacgataata
    15541 tgcggcgcat gttgtaacga atcacgctgc ataccgaacg gttgaccacc gcacaacgtc
    15601 aaaattttgg tatttggcag aaaacgcgcc agccgacgca attcacctgc cacctgatcc
    15661 gccagttcac gcgtaggaca cagcactaaa gcctgggttt gaaatagcga cgcatcaatt
    15721 tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg
    15781 cgaacatctt ttccggcaag gatcgccgga agcgcggcgg cctgcaccgg cgtcatggtt
    15841 aaataaccca acccattaag gtttgtgagt tgggcgggag gcaaaacatt cagggtagaa
    15901 aaagcggtca caatctattc tcgtggtcat cgacgcaaag tcagcaggcg cgtatcctcg
    15961 cagatctacg ctcacgatgc gacaatttaa tcggctcttc atcgggtggt gggtcaggca
    16021 tgggttgcgg gcgagggatc ggatcgggca cgggaacagg atcgccagga atcggttcag
    16081 ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc ctccggtcat tgggtggact
    16141 cttaaagggt agacgctgat aaataacagg caaaaaaaag ccgactcatc aaagtcggcg
    16201 tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg aagtaagaca atatggagcg
    16261 caacgcccat cgcttgacgt tgcattcacc tgcaagagag atattgccct gaatgggtag
    16321 agagtttatt gacttcgctc aaactttacg gcgtttttgt atacagacag ccgggaaaat
    16381 tgcttttgtt acaaccattt actacgatgc aaccataaag caacaccacc aataagaaca
    16441 actaacagaa tacaaaaaat tgaaaatccg aattgccatc caccgccagg gatcccacca
    16501 aggttgacgc caaataaccc tgtcagaaac gtactgggta aaaagaccat tgccatcaac
    16561 gacattgtat aagtacgacg agctaaattt tcctgcatca cctgagcgat ttcatccgcc
    16621 atcacgccag tccgtgctat acaggcgtcg atttcgtcaa gaccgcgccc aaggcgatcg
    16681 gcaatatcct gcatccgacg gcgttggtca tcgctcatcc acggcaaacg ttcactggca
    16741 agacgagcat aaacatcacg ttgcggtgcc atatagcgac gcatcacaat taattgtttg
    16801 cgcagcagag ccaggaatcc acgcggtgga atttgctgat cgagaagatt atcttcgagg
    16861 tcgataattt tatcgtgcag ctgctcgata aattcgctgg aatgatcggt caacgcatcg
    16921 cacacatcaa ccagccaccc cccgcaatcg gtcggaccag tgccctcttc cagatcgctc
    16981 accacatcgt ccagcgccag cactttgcgt tgtcgggtcg aaacaattaa ccgcccgtcc
    17041 atatatacac gcatggcgac cagttgatcg gggcgttcat cggtgctgcc gtttatacag
    17101 cgcaatgtaa tcagcgtgcc ttcaccgaga cggctgactc ggggccgtgt gctctcgccc
    17161 gccagcgcat cacgtacgtt attgggaagc agcggtgttg tcgccagcca ttgggcgcta
    17221 tcatggtgta cataattaag gtggagccag cagggatgcg cttcatcaat cacatctgta
    17281 ttttccagcg gtttaacgcc gcctctacca tccagcatcc atgcaaatac tgcatccggg
    17341 acattaacgt ccgatccctt aatcgcttcc acagtgcctc catcatcaac gcattatttt
    17401 gtagtctagc cttctggccc tgttacgcaa catctcatca ccccattacc ctgaaatgat
    17461 taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg cttttccgta tacaaaccgt
    17521 gtgaagtgtt aaatagcgtc tatcattatc agaattatct gatcatatga cgtggctttt
    17581 ttgcgatcgg atagcaacaa caattgataa aaataatggg atctcaatga ttacgcacaa
    17641 cttcaatacc ctggacttac tcaccagtcc tgtctggatc gtttcgccct ttgaggaaca
    17701 gttaatttat gccaacagcg aggcgcgact gttgatgcaa gacctcacgt ttagtcagct
    17761 acgaaccggt ccctattccg tctcctcaca aaaagaactg ccgaaatacc tctccgatct
    17821 gcaaaatcaa cacgatatta tcgaaatcct caccgttcag cgcaatgaag aggaaacagc
    17881 attaagctgt cggcttgttt tgcgagagct gacagaaaca gaaccggtga ttattttcga
    17941 aggtatcgaa gcaccggcaa cgctgggttt aaaagccagt cgctcgacaa attatcagcg
    18001 caaaaaacaa ggtttttatg cgcgcttttt tctgactaac tctgcgccaa tgttgttgat
    18061 tgacccctca cgagatggcc aaatcgttga tgctaacctc gccgctctca atttctatgg
    18121 ttataaccat gaaacgatgt gccagaaaca tacctgggaa ataaatatgc tcgggcgtcg
    18181 cgtcatgcct atcatgcatg aaatctcgca tttacccggt ggtcataaac ctttgaattt
    18241 tgttcataaa ctggcggatg gttcgactcg tcatgtgcag acctatgccg ggccgattga
    18301 aatttatggc gacaagctca tgttatgtat tgtgcatgat attactgagc aaaaacggct
    18361 ggaggagcag ctggaacatg ctgcccacca tgatgcgatg acaggattac tgaatcggcg
    18421 acagttttat cacattaccg agccaggcca aatgcagcac ctcgccatcg ctcaggatta
    18481 cagcttgttg ctcatcgaca ccgatcgttt taaacacatt aacgatctct atgggcattc
    18541 taaaggtgat gaggtgttat gcgcccttgc ccgcactctc gaaagttgcg ctcgcaaagg
    18601 cgatttggtg tttcgttggg gaggcgaaga gtttgtctta ttgctaccaa gaaccccact
    18661 ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc gtggcaaaag tgagtatttc
    18721 gggcttacca cgctttactg tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat
    18781 cgatgaactg tttaaacgcg ttgatgatgc tttgtatcga gcgaaaaatg atggacgcaa
    18841 ccgcgtgctg gcggcataag ccacggatgc gtctcgtgat caacgactgc gcttagcatg
    18901 gcgctcccag ttttcttgct tcgcctgcgc tgttttacgt agtgcgacgt aacacgcccc
    18961 gctgccgcca tgatgcggta gcgcagtgca atatgcctga acatcatcaa attcggtcag
    19021 ccagcgcgcc acatagctgc ggacaatatt ggcatgcgat ttatcatccc gtcctttgcc
    19081 atgaataatc agcacgttac gcaaaccatc cgccagggct tgttgaatga aactgaacat
    19141 cattttgcgg cactcttcca ccggctggcg caaaagattc aggctcgcct gttgcggata
    19201 tttaccactg cgcagcttat ccagcacccc atgttgcaac ccttcccgcc gaaactccag
    19261 cggccgactt agcgggatga tgtcgagaaa tccagtggtg aggaaattat caagttgcag
    19321 cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga tgccagtgga catctgtagc
    19381 acgtttcagc ggctggacat cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt
    19441 catgtaacct cctatgcacc ttcacacgtc gtgattcaga cgggttaatt gttcatactt
    19501 actgcatcgt tcccttcaca gattgaaaca atactctact tcatacctgt gagtatctgc
    19561 gcaggcggag cattttatac tagcctgtta gcagtatcga acagtttagc ccgagtttaa
    19621 ttcagcacag gctccgatca ggatgattta ttcatagcag gaaccaacaa aaatgcgata
    19681 aataacagac cactgattag cagaaaggta tccgaaaatg ccatagtttg cgcctccctc
    19741 agcatcaatt gactcaataa tttggttgaa gccagtattt ccgatgtctg gtcacttcct
    19801 ccgcgattaa aaaacaaagc acttctggaa atgaagtcat tcaccgtatg gggaacagaa
    19861 accatttttt cacccattct cgaaaaatga aaattagttc gattattgag aatagagcca
    19921 cacagggcaa taccaatcgc gcccccaaga ttacgtgtta aattaaatac accagaggct
    19981 aacttcagcc gttcttttgg aattcccccc agcgttaatg ttacgatggg ggccatcgca
    20041 aactgctgcg aaatccctct aattgcctgt ggaaacagta actcctgcca tccccattcg
    20101 tgggttatag gggtaaaaag atacattgaa aacacaaatc cacccagccc cgccatcaac
    20161 agccatcgca gatttatttt tttacttaac cagaagtaaa agggaacaga aaataactgg
    20221 aaaatacctg ttgtgcaaac ggcaaaacct atctcttcgg catttaatcc gcgaacccgc
    20281 cccaaaaaca caggtattaa atatacagta gagaatatt
//
LOCUS       DAOFSV010000077        20299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.077, whole genome shotgun sequence.
ACCESSION   DAOFSV010000077 DAOFSV010000000
VERSION     DAOFSV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.077"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            194..907
                     /gene="ydiV"
                     /locus_tag="QBH27_002886"
     CDS             194..907
                     /gene="ydiV"
                     /locus_tag="QBH27_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDQ7015175.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            970..2406
                     /locus_tag="QBH27_002887"
     CDS             970..2406
                     /locus_tag="QBH27_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDQ7015176.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWPHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTELKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDNERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2410..2601)
                     /gene="hemP"
                     /locus_tag="QBH27_002888"
     CDS             complement(2410..2601)
                     /gene="hemP"
                     /locus_tag="QBH27_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDQ7015177.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2733..3779)
                     /gene="aroH"
                     /locus_tag="QBH27_002889"
     CDS             complement(2733..3779)
                     /gene="aroH"
                     /locus_tag="QBH27_002889"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDQ7015178.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3936..4769)
                     /gene="ppsR"
                     /locus_tag="QBH27_002890"
     CDS             complement(3936..4769)
                     /gene="ppsR"
                     /locus_tag="QBH27_002890"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDQ7015179.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            5102..7480
                     /gene="ppsA"
                     /locus_tag="QBH27_002891"
     CDS             5102..7480
                     /gene="ppsA"
                     /locus_tag="QBH27_002891"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDQ7015180.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGVDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7537..9183)
                     /gene="fadK"
                     /locus_tag="QBH27_002892"
     CDS             complement(7537..9183)
                     /gene="fadK"
                     /locus_tag="QBH27_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDQ7015181.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(9240..9533)
                     /locus_tag="QBH27_002893"
     CDS             complement(9240..9533)
                     /locus_tag="QBH27_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDQ7015182.1"
                     /translation="MSQNATVNVDIKLGVDKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(9530..10819)
                     /locus_tag="QBH27_002894"
     CDS             complement(9530..10819)
                     /locus_tag="QBH27_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ7015183.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10874..11812)
                     /locus_tag="QBH27_002895"
     CDS             complement(10874..11812)
                     /locus_tag="QBH27_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDQ7015184.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGSAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(11832..12596)
                     /locus_tag="QBH27_002896"
     CDS             complement(11832..12596)
                     /locus_tag="QBH27_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDQ7015185.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            12912..13823
                     /locus_tag="QBH27_002897"
     CDS             12912..13823
                     /locus_tag="QBH27_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ7015186.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(13866..15017)
                     /locus_tag="QBH27_002898"
     CDS             complement(13866..15017)
                     /locus_tag="QBH27_002898"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDQ7015187.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(15031..16626)
                     /locus_tag="QBH27_002899"
     CDS             complement(15031..16626)
                     /locus_tag="QBH27_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDQ7015188.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(16773..17531)
                     /gene="aroD"
                     /locus_tag="QBH27_002900"
     CDS             complement(16773..17531)
                     /gene="aroD"
                     /locus_tag="QBH27_002900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDQ7015189.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(17562..18299)
                     /gene="ydiB"
                     /locus_tag="QBH27_002901"
     CDS             complement(17562..18299)
                     /gene="ydiB"
                     /locus_tag="QBH27_002901"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDQ7015190.1"
                     /translation="MDNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKL
                     VGAINTIVNDDGYLRGYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAI
                     EGLKEIKLFNRRDEFFDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNG
                     TKVGMEPLENESLVNDISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLW
                     QGAEQFTLWTGKDFPLEYVKQVMGFGA"
     gene            complement(18303..19004)
                     /locus_tag="QBH27_002902"
                     /pseudo
     CDS             complement(18303..19004)
                     /locus_tag="QBH27_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19231..>20299)
                     /locus_tag="QBH27_002903"
     CDS             complement(19231..>20299)
                     /locus_tag="QBH27_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015191.1"
                     /translation="SLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTN
                     NIIIAYVFGFLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIIS
                     LLVWAELWFGWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIID
                     LASYSLYGYISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPL
                     IRNTVRPTTLLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMA
                     ERFPYAKGKATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFI
                     GLRSRKETRHHTLKENIAPGG"
BASE COUNT         5095 a         5004 c         5022 g         5178 t
ORIGIN      
        1 agtagtattt tgcagacaga aattccgtaa atggattatt cgtttataat caggataatc
       61 taaaaaaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt actccttctg
      121 taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac atccttcgag
      181 agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt gctatttcct
      241 gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc atttctccag
      301 tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta ccgaggagca
      361 acattggcaa ttattttctg aacagttgga attactaaaa tcatgccaac acttttttat
      421 tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat tattattaga
      481 cagggataat ttcgcaggcg aactattaaa atatccgttt attgaactgt tgattaatga
      541 gaattacccg catttcaatg aaggaaaaga taatcgcgat cttttatcct tatcgcaaat
      601 gtatcccctt gtgttaggta atttgggagc aggtaatagc acaatgaaag cggtgtttga
      661 tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa tcacgcatcg
      721 ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt gcaattgcat
      781 catcgcgggg ggcattgata cgccagaaat attggcacag attataccct ttgatttcca
      841 tgctctccag ggatgcctgt ggcctgcggt tcctataaat cagataacga cgttggttca
      901 gcgataaccc tcctgtttgc tggtgtttag gacgagagta accgtctaca ctatcaaaca
      961 ggaggatcta tgaccctgtc ttttattacc cgctggcgcg atgaattgcc agaaacctat
     1021 acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa taccgaactg
     1081 gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt ctggggcggc
     1141 gaaaacctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca tcagtttggc
     1201 gtctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca actgcttgct
     1261 gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg
     1321 ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag tgaggcgatg
     1381 cattatctgg gcattccgac gacccgcgct ttaagtatcg tcaccagcga ttcgccagtg
     1441 tatcgggaaa cggtggagcc aggcgcgatg ctgatgcgtg tggcaccaag tcatctgcgc
     1501 tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg tcagttagct
     1561 gactttgcca ttcgtcatta ctggccacat cttgcagatg atgaggacaa ataccgtctc
     1621 tggtttaccg atgttgtcgc acgtaccgcc tcgttaattg cccaatggca gactgtcggc
     1681 tttgctcatg gggtgatgaa taccgacaac atgtcgctgc tggggctgac gcttgattac
     1741 gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca ctcggatcat
     1801 caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt acagcgtctg
     1861 gcgcagacat tgtcaccatt tgttgccgta gatggcctga atgaggccct ggacagctat
     1921 cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg cttcatgacg
     1981 gagctaaaag aggataacgc gctactgaat gaattattca gtctgatggc gcgagagcgc
     2041 agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagcacag cgcggcgtca
     2101 ccgctacgtg atgagtttat tgatcgtgca gcatttgatg actggtttgc ccgttatcgg
     2161 gggcgtttgc aacaagacga gattaccgat aatgagcgtc agcaactgat gcaaagcgtt
     2221 aacccggctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc ggcagaaaag
     2281 ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga
     2341 gatgatgact atgtcagtcg tccacctgac tggggtaaac ggctggaagt cagctgctcg
     2401 agttaaaggc tacttggtca acagcagctt gccagcctga gttttacgga gcagatattc
     2461 ttgtccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct ggctggtaat
     2521 tcgccggatg ggctgcgggg aagcggtctt gtgattggca tccgtttcag gcgtgagttt
     2581 tctgctatcc gtataacgca taaaaattaa taataattta tataacaatg attcttatta
     2641 tcaatattat ggagggccag agcaagcagt aatttgttga gaagacaaca agttgcgcag
     2701 aaatgcccat cctgtcagga atgggcacgc attcagaagc gggtatctac cgcagaggcg
     2761 agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt aatggattga
     2821 ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc
     2881 gccataattc cggcaatcgc cgtagagcca ttgcggattt gctgacaaat atcctcacaa
     2941 acttctaact ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc caccaccaga
     3001 tgttcaggca aatcaaattc gtgcagcgta tcgcaggctg cggcgatatc atcggcatga
     3061 taattcggtt ttttgccgcc acgcataata atgtggccat acgggttgcc gctggtctga
     3121 tagatggtca tctgaccgtt tttgtctggc gagaggaaca tatggctagc gcgggctgcg
     3181 cggatagcat ccacggcaat ccgcgtgttg ccatcggtac catttttaaa acccaccgga
     3241 caggagagtg ccgaagccat ttcgcggtgg atctgacttt cggtagtacg tgcgccaatc
     3301 gcgccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag gaactcagtc
     3361 gcggtaggga cgcccagctc atttacctgt aaaagtaatt tgcgcgccag ctccagaccg
     3421 tgatttaccc gatagctacc gtttaaatct ggatcggaga tgagtccttt ccagccgaca
     3481 accgttcgtg gtttttcaaa ataggtgcgc attacgattt ccagccgtga ctggtactgg
     3541 ttgcgcagcg actgcagacg ggtggcgtac tccatcgcag cggtgagatc gtggatcgag
     3601 caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt ttcaattcta
     3661 cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt
     3721 tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt
     3781 acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat gcgcatcacg
     3841 ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc
     3901 atgataacaa aaaaacaagc taatgcacta gttctctagt acattcggcg gctaaggccc
     3961 atgatatcga ggatcttggt ggcaatctct tctaccgaat aattggtact gttaatccac
     4021 gggatctgat ttttacggta caacgcttcc acttccgcga cttccatcct gcactgacga
     4081 agcgaggcat agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg
     4141 tcgatagtca ggccgaacaa tttatgctga agtggtttga gcgacgcggg tagcaccaga
     4201 ttatccatat catcggcaat aaaggggtag tttgccgcgc ggataccaaa ttgcattgcc
     4261 agatacagac tggtgggggt tttaccacag cgagaaacac cgagcaggat cacctgagcc
     4321 tggtccagat tgcgcaacga aataccgtca tcgtgggcaa gggtgtaatc aatcgccgca
     4381 atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg atgagcaatc
     4441 ggcgttggat ccagtttcat ctcttgttgt agtggggcaa ccagcgcctg aacgatatcc
     4501 tggcaaaagc cttcactttg caagatgata gcgcgaatct ccggcaacac gatggagtag
     4561 aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc cttcactgcc
     4621 cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt gacgggaaat
     4681 tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc agaaatataa
     4741 aaaacgtggc gatcaacagc attatccatt ttgttcttcc cgtgatgcag acataattct
     4801 atgaaagcat aaattaaaaa cgcacagaag cgtagaacgt tatgtctggt ttataaaatg
     4861 aaacttaaat tttatttttt atgaaaacag catttcattt ttatggtttc gtttataccg
     4921 atggtttatg tggaaattgt cgaagagagc agatttgcgc aacgctggga tcagtcttaa
     4981 aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatctgg ttaaatatgc
     5041 aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc
     5101 gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga
     5161 tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta acctttccgg
     5221 aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct
     5281 ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga
     5341 tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt
     5401 ccagcctgag ctggaaaacg ccatccgcga agcctatgca cagctttccg ccgatgacga
     5461 aaacgcttcg tttgcggtgc gttcctccgc tactgcagaa gatatgccgg acgcttcttt
     5521 tgccggtcag caggaaactt tcctcaacgt tcagggtgtt gacgccgttc tcgtggcagt
     5581 gaagcatgta ttcgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg
     5641 ttacgaccat cgtggcgtag cgctctccgc cggtgttcag cggatggtgc gctccgacct
     5701 cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt
     5761 tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggtgcggtta acccggatga
     5821 gttttacgtc cataaaccga cacttgcggc gaatcgcccg gctatcgtgc gccgcaccat
     5881 ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa
     5941 aatcgaagac gtaccgcagg aacagcgtga catcttctcg ctgaccaacg aagaagtgca
     6001 ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccga tggatattga
     6061 gtgggcgaaa gatggtcaca ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt
     6121 gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc
     6181 cgaaggccgt gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtta tccatgacat
     6241 cagtgaaatg aaccgcatcg aacctggcga cgtgctggtt actgacatga ccgacccgga
     6301 ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aatcgtggcg gtcgtacctg
     6361 tcacgcggcg atcatcgctc gtgaactcgg cattccggcg gtagtgggct gtggtgatgc
     6421 aacagaacgg atgaaagacg gcgagaacgt cactgtttct tgtgccgaag gtgataccgg
     6481 ttacgtctat gcggagttgc tggaatttag cgttaaaagc tccagcgtag aaacgatgcc
     6541 ggacctgccg ttgaaggtga tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc
     6601 ctgcctgccg aacgaaggcg tgggccttgc gcgtctggaa tttatcatca accgtatgat
     6661 tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga
     6721 aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac
     6781 tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc
     6841 tgattttaaa tcgaacgaat atgccaatct ggtcggtggt gagcgttacg agccagatga
     6901 agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca gcttccgcga
     6961 ctgcttcgcg ctggagtgtg aagcagtgaa acgtgtgcgc aacgacatgg ggctgactaa
     7021 cgttgagatc atgatcccgt tcgtgcgtac cgttgatcag gcgaaagcgg tggttgagga
     7081 actggcgcgt caggggctga aacgtggtga gaacgggctg aaaatcatca tgatgtgtga
     7141 aattccgtcc aacgccttgc tggcagagca gttcctggaa tatttcgacg gcttctcaat
     7201 tggctcaaac gacatgacgc agctggcgct cggtctggat cgtgactccg gcgtggtgtc
     7261 tgaactgttc gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc
     7321 cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccatccg accacgaaga
     7381 ctttgctgca tggttgatgg aagaggggat cgatagcctg tctctgaacc cggacaccgt
     7441 ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatacc cggcggcgtt
     7501 ttgtcgccgg gtttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc
     7561 ctgagttaat cgccgcataa tatctttcct taacaaaaac ttttgtattt tacctgagac
     7621 agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg
     7681 tttacggcta aaaaaagcca ccacctcttc cagcgataat gaatgatgcg gcgctttcag
     7741 cacgacataa gcgcatgaac gttcacctaa acgttcatcg ggcatcgcaa ccacacaggc
     7801 atcgtgaatt ttaggatgct gcaataaaat atcttctact tcacggctgc taatattttc
     7861 gccgccgcgg acaataatat ctttcttgcg cccggttatt tttatatagc cagcctcatc
     7921 catgcggcag agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa
     7981 ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgaggctt cttcaccttc
     8041 gcaacctggc ggtaaagtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc
     8101 agcgtaaccg tcggtgtgca taaagcgcgg caaaggatcg tcgagattca ccaccgcatg
     8161 cggcgaactt tctgtggaac cataaacact taataattta atgccgcgct gctggcattc
     8221 tcgcgccact tttttgggga ttgtggtacc tccgcaaaga aagaagcgca gcgctgaaag
     8281 gtctgcgggt tgtttctcta gtaaattcaa aagatcatag acaaacggcg ttgcgccgag
     8341 catacaggtg caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc
     8401 taacaacacg ctgcgagcac caatcaaaaa aggtgctgtt acgccatgca gaaagcccgt
     8461 tgcgtgacca agtggcgcag gcatcataaa gacatcctgc caggtcagat ttagccgcgc
     8521 gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacac cctttggcag
     8581 accctcggtt ccggaggtaa atagcacagc ggctaattca tcgccgtggg tcgttatcgc
     8641 cgtggtcaga ggagtattgt cggcgagaat ctgacttaat gagagggaag aggtggcggg
     8701 agccagtttg tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag
     8761 gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg
     8821 acacttattg agcacccaca ccagttctgc ttcccgccag gaaggtaaca gcggcacgga
     8881 aaccgcaccg gttttcaggc aggcaagata gataacggta aattcacacc agcctggcag
     8941 ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca
     9001 gctcgcggcg tgatcgagcg cgctataggt gtacgttgca ccatgattat cgaccacggc
     9061 aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc
     9121 ccataaccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta atgtcacttt
     9181 cattttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg gatgcatgat
     9241 tagccgtagc gaaattcaat accgaaggtg cctgcgggat attgccactg ttcgagaata
     9301 gtgttgccgc acagcacccg acaggtgccg cactccagac aaccggcgga atcgaaatga
     9361 atgtttcctg cgtcatcctg cttataaagt ccggcagggc aagctttcat taatttacgg
     9421 aattcattga tatcgggatt tgccgccaaa atgatatgcg ggtggccctc atcaacatgg
     9481 aatttatcga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg
     9541 ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc gcgtgtccca
     9601 tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacata tcgttcatga
     9661 tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga
     9721 tcttgcggaa atgctgcata tcgcgcataa cacaactttg ttccagctca cgtttgtatt
     9781 gcgccagact gctggcggag aaatccgcgc gttctttggc ggcgatcacc gttgtggcgg
     9841 cagcctgagc tgatgcaatg gctaaatcca tgccgcgaac tgtaaaaccc aaattcaggc
     9901 agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccagctgc ggcaccattg
     9961 ccagaccgcc ctccggcacc atatgcgcgg aatattcaag cagtttgccg ccgctaatca
    10021 gcgggcgaat agcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg
    10081 catgggcgat atcacccaat ccacaaacca gccccaacga tacggaatcc ttgttggtat
    10141 agaggaatcc gccgcccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac
    10201 cttcctcgcc cgtaacatta aagcgatcgt tgatctgttc tggtgtcagg cctataacct
    10261 ctttaacacc aacagcgtaa tgatgcggat tggaagcgga aaccattccc agcgagcggc
    10321 caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga atatcatccc
    10381 cggcctggac gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg cgtacgcccg
    10441 ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac
    10501 gcaatacggt ataagacgcg tgttgcggaa catctggttg ctcgcggtga aaatcgaggg
    10561 taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg
    10621 gcgctgatgc tgcaaagcct ggaataattg cttcaagtgt gtgggcataa agacgcccgc
    10681 cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg
    10741 ctcgcgccat gaccagtgcg gcaacgctac cggcaacgcc cgcaccgacc acaatggcat
    10801 caaatttgtc atccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt
    10861 ggttgaaagg gaattagcga ttcaactggc taatcagggc agggacgact ttgtagatat
    10921 cgcccaccag accgtagtcg gcatagttga agattggcgc atttttatct ttattaatgg
    10981 cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg gatctgcccg gagatcccca
    11041 gcgtcaggta gagatcggat ttcagcaaca cgccggagac accgatataa cgttcacgct
    11101 ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca ttcagcactg
    11161 ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa
    11221 ccacacgttt tgctttgctc aggtccacgc tgcttgccgc tttcgcacgg cgttcgcgac
    11281 agagaatttc atgacgcggg gcaacataag gtaccgtttc tgtcgggcac tgatgagaga
    11341 tatcacgggt gcacggttcc tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt
    11401 tgatcttttc ctgagcgaac gctaacccgc catacatccg gtgttcggcg caaatgtgac
    11461 catcgacaat atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt tgcacactta
    11521 accgtgcggc cagagcttta ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt
    11581 tatctttcag cagggcagca atgctttcgg cgtaattttc agtacgttgc agcgcgtcgt
    11641 tttgctcaag aacataaatg cattttggcc cataaggcat aactgcctgc gcctgctcgg
    11701 tattttgtac aatggcatac acctgttggc cccattgctg agcgctgcca aacagttcag
    11761 cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc atcgtttctt
    11821 ctccgtaggc ttcagttcag ggctttcttc agatgttccg ccagctcggc aatggcctcc
    11881 ggcgaatcgt tatcgagaat gatgtgctta cgttctgttt gcggcggtac gcgaatgccg
    11941 gtcagttcag caagtggtgc gctctggctc cagccaatat cacttgcctg ccactgattt
    12001 accggttttt tacccgcgcc gagaatggct ttcatcgaag gaatacgtgg cacgttaata
    12061 tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac atcatcttca
    12121 agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa tagcactcac tgcattaatc
    12181 accggaagtt gcagaatttc tccgaccagc aagccaacct gctgggcata aaggtcgccg
    12241 gaaccttcac caaagatcag taaatcgaag ccgatctttt caactgctgt cgccagcgcc
    12301 tttgcggtat cgagaggcag tgcatgctca agttgtgcat cctgcaccat atacaggctg
    12361 tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa agagccgccg
    12421 acggtcagcg cggctatctc atcgtcatct gttgcaagct ggcttgcagc ttcaatggca
    12481 ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt gtattctgga
    12541 gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat tttcatgatt
    12601 tctcctgtga gtcgcatacg gaaccatctc cgatggaaag taaaaatggc ctgaaaatag
    12661 tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc aacaatcttg
    12721 caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga
    12781 ccgtactatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta
    12841 taaccgcaat acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata
    12901 gggaggttgt catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg
    12961 gtaaaacgcc acggctttca cgtttcgcat ttagcgatga tccaaaatgg gagtctggac
    13021 atcacgttca tgacaatgaa accgagctga tttacgtcaa gaaaggggta gcaaggttta
    13081 ccatcgattc ttcgttatat gtcgcgcatg ccgatgacat tgtggtgata gaacgtggca
    13141 ggttgcatgc ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt
    13201 acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat tcttgcccgg
    13261 taattgccgc aggacaggga aaagaagtca ttaaaacctt atttaatgag ctaagtgtga
    13321 ttttgccgca aagtaaaaat agccaaacat cttcgttatg ggacgcattt gcctatacgt
    13381 tagcaattct ttactacgaa aactttaaaa atgcttatcg ttcggagcag ggatatatta
    13441 aaaaagatgt tctgataaaa gatattcttt tctatctgaa taataactat cgcgaaaaaa
    13501 tcactttaga gcagttatcg aaaaaatttc gtgccagcgt tagttatatc tgccatgaat
    13561 ttaccaaaga gtatcgtatt tcccctatta actatgttat tcaacggcgt atgacggaag
    13621 cgaaatggtc actcactaat actgaattat cacaggcaga gatctcctgg cgtgtgggtt
    13681 atgaaaatgt cgatcacttt gccaaactgt ttttgcgcca tgtcggctgt tcgcccagcg
    13741 attaccgcag gcaatttaaa aactgttttg cggaacaaga aatcctatct gaatttcctc
    13801 aaccagtaag tcttgccgga taaatctggc ggcgcagttt gttaagaagc tgcgccgcct
    13861 gcagattatt tgttctgata atctttcagg atctgccgac ctgctacgta aatcataatt
    13921 tcgtctgtac cgccgccgat acgttcacaa cggacatcac gccagaagcg ggagacgcgc
    13981 gcctcatcgg tatagcccag accgcccatg atttgaatcg catcatcaat gacttccatt
    14041 gcggtacgtg cgcagtacag ttttgccagc gccgcgctgg tgcgcagtga ctgatgctga
    14101 tcggcttgcc atgccacttt caacaccatg ttgcgcatgt tgtcgatctt aatcgccatc
    14161 agcgccagtt tttcctggat catctggtta tgaccaatgg gcttaccaaa agcgatacgc
    14221 tggttggcat agcgggcggc atcttcgaat gcgcattcgg caaagccggt gctgcgcgcg
    14281 gcgttgatca ggcgctccat ctcaaagttg tacatcacat tgaggaaacc cattccttct
    14341 tcgcccacca tgtcgctctc ttcaacttcc acgttgtcga gatagacttc gcaggtgctg
    14401 agcatatgcc agccgatttt atgcagtgga ttgattttaa tgccgggctt actggagtcg
    14461 acccaccaca gagtaaaggc ctttttcgga tctttcggtt gcggatcgcg cgccaacacc
    14521 agcatatatg gatactcttt cgcaccggta ataaaggttt tctgtccgtt gatgtaaacc
    14581 ttgccgtttt tacgcgtgta agtggtagtg gcactgttgt tgtctgagcc tgcgcctggc
    14641 tccgtcaatg ccagggcata ggcgggatca cccgtttcca gggtgctctc tgccgtttta
    14701 cgtagctgct ctgcagaacc gaaacggcgc atactgtgaa tacattgacc gttggtaatc
    14761 aaaaatgccg gagcaccgca ttttgacact tccatcagcg ccagcatttg ggtgacgtaa
    14821 tccgcaggga taccaccaaa ttcttccggc acgccaagca tggaaatacc gttatccgcc
    14881 agtgcccgca taaactcacg cgggtatgtc ccgttttgat cgcaggtgcg gaaatactct
    14941 tccggaaagt tagtcgtaat cagttcgcga atactggcca gcagcagttc ttgttcttca
    15001 gttaaagaga aatccatcat tatactcctg ttaatgagcc gcttcaggca ggacaaaacc
    15061 catcgccgca tcgataaata atctttcgtc catcagtttg agttctggcg aaatcactgg
    15121 ggtgaaatcc attttgtcga gaatatcttt ttgtaaatcg acgccaggag cgatttcgat
    15181 taaatgcagg ccgtcttctt ttagcgtgaa tacggcgcgc tcagtgatat aacgaacatc
    15241 cagccctcgc tcgagagcga tttttccgct gaaagttatt tccggtagtt cccgaataaa
    15301 tttcttcacc cgtccttcct ggacgatatt taatttgccg tcggtaattt ctgtttttaa
    15361 actgcccgca gttaatgtgc cgcagaaaac gattttcttc gaagtggcac tgatatcaat
    15421 aaatccaccg gtgcccatga ttttaccatt gaatttatgc acgccgacgt taccgtgctg
    15481 gtcgacttca gcaaaactca aataacaaac atccagacca ccaccgtgat aaaaatcaaa
    15541 ctgggacgtc atatccagaa tggcgcgggt attcacgttc gcgccaaagg cgatcccctg
    15601 tgaagtaata ccgccaatcg gacctgtttc taccgtcaga ataaagtcat cagcacaacc
    15661 ttcttcccgg gcgaccaggc caatgccgtc agcaataccg acgccgacat tccccactgc
    15721 gcctttgcgc atttcgaata atgcacgccg tgcaactaat ttacgttggt ttaggggcag
    15781 gctaagtttg gtgctgtcat caagggtgaa gtcaccagaa ataaagcggt taaccggcgc
    15841 accgccatac agttgggttt gatccggatc gaccaccaca atatccacca gataacccgg
    15901 aatacggaca gatttaggat gcagcgtggc tttcttaacc attttctgca cctgcatcat
    15961 cacaataccg ccgttattgt gcaccgcctg ggcaataacc aatgcgtcga gatacatcac
    16021 ctcatcttca aaagtggcgt agccttcgct gtcgcaggtg gtagcgcgaa tgaaggcgat
    16081 atctggcgca atcgctttgt aatagagata ttctttgtta tcaaactcga ccagtttaat
    16141 caggtcttct ttagtgactt cattcagttt gccgccttgc tggcgtggat cgacaaatgt
    16201 tccgatgcca atatcactaa taataccagg ctggtgggct gcggcggcgc gtaaggtttg
    16261 tgtaagtaca ccttgtggat agttataagc aataatttta ttttgttctg cgagatcaga
    16321 aatacgcggt gattgtcccc agtgaccgca taatgcccat ttcaccaggc cttcttgcgc
    16381 cagcggacta ataccacggt cggcgcgatc gccaagccct gttggactaa taatcgataa
    16441 attacgtggt gtttgagtct gtttatattt atcagcaaga gcagtaatta acgtggtggc
    16501 ttccagaata ccgccgccag cgcctaacac acaaagtgtt gcttcgtcgg gaatataatt
    16561 caccgcttcc tgtgccgata ggaccggcac tcgtccatta atacgaggtg gttttacagg
    16621 tttcattgtt aattcctgat tcaggtgtga ttttccctac gccagatgga gaattagcgc
    16681 acagagactc acgaaatatt tttattaaat acggttaaaa atactttttt tagttcggcg
    16741 ggagagggta ttcccgccga aaatattatt gcttatgcct gatgtaaaat agttaatacc
    16801 gtacgcaaat catttaccga gatttgccct ggcgcagagg ctttttttac cgcaccaaaa
    16861 gttgccgccg acccaaatac ttcaccagcc agacgagaaa ttacgccagt ttttgccatc
    16921 gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt cgcggcaagc
    16981 aacgtcagca catcgctggt actttgcggc atcagcgcaa tcttaggaat atcggcgtcg
    17041 aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt tttatggaag
    17101 tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac ggtttcttta
    17161 acctgatcat cgccggtaaa taactccaga tcgatcatat caaccaggcc gctgtcgatg
    17221 gctgcacgat tgagtgcaat ataagcctcg gtggaaatcg cctgctcgcc gccttctttg
    17281 gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag aatttttgct
    17341 gccgccatga cagactccac attggagagg tcggcatagt ggtccacacg ccattccaga
    17401 atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcacgctggc gatatctttc
    17461 gccatcagcg agacgatgat tttaggtgcg cccgtaccaa tgacgagatc ttttacagtt
    17521 acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc gaaccccatg
    17581 acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc
    17641 ccttgccaca acaacatgcc gtatccatca atcgttttgc aacccgcttg ttgcgcctgc
    17701 tgcaataact tcgtcatatg agggttatac acgcattcag tgaccagaag tcccgggtgt
    17761 aacagactga tatcattaac caatgattca ttctcaaggg gttccatacc cactttggtg
    17821 ccattggtta aaatgtcggc ggaagccagg gcttctgcaa aggcttgctg atcggcgaga
    17881 tcggtgactg tcacgacgca atcggtgttt tcgttaaccc gctgcgcgaa ggcgagagct
    17941 ttatcgaaga actcatcccg acggttaaag agtttaattt cttttaaacc ttcaattgcc
    18001 ccctgcgcgc caattgccgt tgaggcacca ccggccccta atagcaccat cgttttgcct
    18061 ttgatatcaa aaccgctctc tttaatggcg cgaatatgac ccgtgccgtc ggtgttatag
    18121 ccacgcagat agccatcatc atttacgatg gtgttgatgg cacccaccag tttggccgct
    18181 ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcattga tacaccagtt
    18241 ccgcgcattt tgagggcttt taatccttca attgctccgg gaaaactatc gttatccact
    18301 cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac tgatgaaaca
    18361 ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc tacgctggcg
    18421 tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag tacaaacaga
    18481 atacccggaa taatcagccc atagccgtac cagatgttat tgagcaacat atagctcacc
    18541 agcattggat agaacatttg cccgaatgac accatcgctt taaccagtat gaccgccgaa
    18601 ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc cagcgctgag
    18661 ttagcgatac ctacgcacac tgccagacag taggcgagag ttaaattcgg gcaagcagga
    18721 ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc ccgacgacca
    18781 aacttatcgg agatcactcc gaagaataaa atactgatca atcgccccaa tccgataccg
    18841 gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccaa agatgtcatg
    18901 ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta aatacaaaga
    18961 acagccagga taaatggcgt gctgaaagcc ttattttgag acatgttcac agctccttgc
    19021 tgaaagataa tttcgttacc gtacgtattg ttctccgacg gtttacataa ttactatact
    19081 gaagatgatt ttccctgggt acatggcata aagtttatgt ttccaggaat attgcatgtc
    19141 atagtttgtt tttgatcgct attttcctta gttagcgcag aaagagttat ttatccgtaa
    19201 gatcttgcat aacctgaaaa gaatattgca ttacccaccc ggagcgatat tttcctttag
    19261 cgtgtgatgc cgcgtttctt tgcgactgcg taagccaata aacagtgcca gtataaagcc
    19321 gatagcagcg atggcggtat cgaaccacat aatatcggca atacttcttt gcgaaagatg
    19381 agccgtaatc aacggaatgg taaatgttgc aatgctgccc gcactgtaat agatccccgt
    19441 agctttacct ttagcgtagg gaaaacgttc cgccattaac gtcaggccaa tttgtacaac
    19501 accgcctgcg gaggtaaaac cgatgacgaa agcaaatatt atcaccacat aaaatgtggg
    19561 atgcaggcag acggtgagca gggcgataaa tgagataaag gtgtacagca tcagtaatgt
    19621 tgttgggcga acggtattac gaatgagtgg agcagtaata aatacacaca gcagcgaacc
    19681 cacggtgtag atactgagta atttgatcga catggtgtat gacatgccag cgacaaattg
    19741 tccgtattgt gccagccact ggctaaccag ataaaacgtt gccattgaga tatagccata
    19801 tagggaataa ctggctaaat cgataattga acagcgatgt tccgtagagc tggtggtttt
    19861 ctttatgaca ggtaagtgac gacccggatg gggtgggaac gtacaacggt ataaaaacag
    19921 agcgttaata aacataatgc ctgcggcaat cataaaggac caaccgaacc acagttcagc
    19981 ccacactaac aggctgataa ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt
    20041 aattaaaata ttggctgtac ccggtgagcg tggaaaagct tccatcaaac tgggataggt
    20101 gcctgcatcg agaaaactgt ttgccattcc cgccagaaag ccaaaaacat aagcgataat
    20161 gatgttattg gtgtgcagga tgccaaaaaa gaaggccata tagcagcaca tcccgagcat
    20221 gataaaaggg cggcgaccaa agcgatcgga taataatcct gcaaaaagca agacacttaa
    20281 tcgaccaatg cccagtgat
//
LOCUS       DAOFSV010000078        19998 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.078, whole genome shotgun sequence.
ACCESSION   DAOFSV010000078 DAOFSV010000000
VERSION     DAOFSV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.078"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(26..598)
                     /gene="hdeD"
                     /locus_tag="QBH27_002904"
     CDS             complement(26..598)
                     /gene="hdeD"
                     /locus_tag="QBH27_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDQ7015192.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            853..1185
                     /gene="hdeA"
                     /locus_tag="QBH27_002905"
     CDS             853..1185
                     /gene="hdeA"
                     /locus_tag="QBH27_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDQ7015193.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            1301..1627
                     /gene="hdeB"
                     /locus_tag="QBH27_002906"
     CDS             1301..1627
                     /gene="hdeB"
                     /locus_tag="QBH27_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDQ7015194.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1691..2338
                     /locus_tag="QBH27_002907"
     CDS             1691..2338
                     /locus_tag="QBH27_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDQ7015195.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2390..3160)
                     /locus_tag="QBH27_002908"
     CDS             complement(2390..3160)
                     /locus_tag="QBH27_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015196.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3157..4113)
                     /gene="chuU"
                     /locus_tag="QBH27_002909"
     CDS             complement(3157..4113)
                     /gene="chuU"
                     /locus_tag="QBH27_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDQ7015197.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4198..4821)
                     /gene="chuY"
                     /locus_tag="QBH27_002910"
     CDS             complement(4198..4821)
                     /gene="chuY"
                     /locus_tag="QBH27_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDQ7015198.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4821..5315)
                     /gene="hutX"
                     /locus_tag="QBH27_002911"
     CDS             complement(4821..5315)
                     /gene="hutX"
                     /locus_tag="QBH27_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDQ7015199.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(5328..6665)
                     /locus_tag="QBH27_002912"
     CDS             complement(5328..6665)
                     /locus_tag="QBH27_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDQ7015200.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLVQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDVCAHYTDALIREIELEAD
                     SVLHQSGPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(6685..7599)
                     /gene="chuT"
                     /locus_tag="QBH27_002913"
     CDS             complement(6685..7599)
                     /gene="chuT"
                     /locus_tag="QBH27_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HDQ7015201.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8283..10265
                     /gene="chuA"
                     /locus_tag="QBH27_002914"
                     /gene_synonym="shuA"
     CDS             8283..10265
                     /gene="chuA"
                     /locus_tag="QBH27_002914"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDQ7015202.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10314..11342
                     /gene="chuS"
                     /locus_tag="QBH27_002915"
     CDS             10314..11342
                     /gene="chuS"
                     /locus_tag="QBH27_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDQ7015203.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(11399..11929)
                     /locus_tag="QBH27_002916"
     CDS             complement(11399..11929)
                     /locus_tag="QBH27_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7015204.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(12082..12648)
                     /gene="slp"
                     /locus_tag="QBH27_002917"
     CDS             complement(12082..12648)
                     /gene="slp"
                     /locus_tag="QBH27_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDQ7015205.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12838..13217)
                     /locus_tag="QBH27_002918"
                     /pseudo
     CDS             complement(12838..13217)
                     /locus_tag="QBH27_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13834..14259)
                     /gene="arsC"
                     /locus_tag="QBH27_002919"
     CDS             complement(13834..14259)
                     /gene="arsC"
                     /locus_tag="QBH27_002919"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDQ7015206.1"
                     /translation="MSNITIYHNPACGTSRNALEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            14594..14677
                     /locus_tag="QBH27_002920"
     CDS             14594..14677
                     /locus_tag="QBH27_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDQ7015207.1"
                     /translation="MIDKAIIVLGALIAQLELIRFLLQLLN"
     gene            complement(14731..16083)
                     /gene="gorA"
                     /locus_tag="QBH27_002921"
     CDS             complement(14731..16083)
                     /gene="gorA"
                     /locus_tag="QBH27_002921"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDQ7015208.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCIGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(16155..16997)
                     /gene="rlmJ"
                     /locus_tag="QBH27_002922"
     CDS             complement(16155..16997)
                     /gene="rlmJ"
                     /locus_tag="QBH27_002922"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDQ7015209.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            17200..19242
                     /gene="prlC"
                     /locus_tag="QBH27_002923"
     CDS             17200..19242
                     /gene="prlC"
                     /locus_tag="QBH27_002923"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDQ7015210.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            19250..>19998
                     /gene="rsmJ"
                     /locus_tag="QBH27_002924"
     CDS             19250..>19998
                     /gene="rsmJ"
                     /locus_tag="QBH27_002924"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDQ7015211.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPAQLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
BASE COUNT         5118 a         4759 c         5044 g         5077 t
ORIGIN      
        1 atgatccagg tggaaatagg gataattatt gctttttaac gaacaaactg gcgaagctga
       61 acagactggc ggcgctaaat atcagttcaa ttcccaccag tgtggaaacc agcgttaccg
      121 acaccatcgg cgtagcacca aggaatatcc aggcaatgac gatatccagc acaccaataa
      181 cgagctgtag ccaactgcct ttcattgaac gctgacgata ccaactcatc aggcgaataa
      241 cccctgccac acagaacaaa ccggcaataa atgccgcaat ggcaaaaatg cccagctctg
      301 gtgcgcggat gaagaaatag ccgatcaata aataggcgac tgcgacgagg aaaccggata
      361 ataccggcca gaaattatga ctgcggttgc tgaatagccc gacaataagc gcaatgcccg
      421 agcagattaa taatgcaccc actactgtgc ttaaaatgtc gccagagacg aacggaaaac
      481 tgatacacag caacccgacg ataaacagca gcacggcaat aaactggatt gctctgcgat
      541 gttttttaag catctccaga tcaaacttca aaattgttgc cttatctata tataacatag
      601 aaccacccta taaaattaag aagaaaatcc cctgctatca atctatgcca aaaacgcgtc
      661 taagaatgca gtcgatttaa taaaaatttc ctaattacag tatctgatgc atctgtaact
      721 cattgtattg aaataaaaat atctgatttt gatattttcc atcaacatga catatacaga
      781 aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac ggctctttca ctttatagtt
      841 gaggatatta cgatgaaaaa agtattaggc gttattcttg gtggtctgct tcttctgcca
      901 gtagtgagca atgcagcgga tgcacaaaaa gcagctgata acaaaaaacc ggtcaactcc
      961 tggacctgtg aagatttcct ggctgtggac gaatccttcc agccaactgc agttggtttt
     1021 gctgaagcac tgaacaacaa agataaacca gaagatgcgg ttttagatgt tcagggtatt
     1081 gcaaccgtaa ccccagctat cgttcaggct tgtactcagg ataaaaaagc cagctttaaa
     1141 gataaagtta aaggcgaatg ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt
     1201 acatcgtatt tccttgcata ttgaacaggc cggaatatct tctttaaaag cagctattcc
     1261 tcctgtttgt atataatctc tatattgaat gggttacaaa atgaacattt catctctccg
     1321 taaagcgttt atttttatgg gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc
     1381 gctggcagcc aatgaatccg ctaaagatat gacctgccag gaatttattg atctgaatcc
     1441 aaaagcaatg acaccagttg cgtggtggat gctgcatgaa gaaacagtat ataaaggtgg
     1501 cgataccgtt actttaaatg aaaccgatct cactcaaatt cctaaagtga tcgaatactg
     1561 caagaaaaac ccgcagaaaa atttgtatac cttcaaaaat caagcatcta atgacttgcc
     1621 gaattaatga ggtacaagta aaaaggagta gcaagttgag ccatcttgct gctccttttt
     1681 gcatttttat atgacagcag aatttattat acgacttata cttgcggtaa ttgcctgtgg
     1741 cgctattggc atggaaagac aaatgcgcgg caaaggagca gggttacgca cacatgtatt
     1801 aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct
     1861 gtctttaggt cacgtcggac tagaccccag ccgtattgct gctcaggttg tgacgggcgt
     1921 cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa aatattgtag gtctgacgac
     1981 ggcagcggat atctgggtga ccgccgccat tggtatggtt attggcagcg gtatgtacga
     2041 actgggtatt tatggttcag tgatgacctt gctggtgctg gaagtcttcc atcaattaac
     2101 cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg gtaatacggt
     2161 atcaatgctc gactggttta agcagcaaaa aataaaaacg gatttagttt cgttgcagga
     2221 aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacga cgtcgataga
     2281 agatctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa ttagctaatc
     2341 tgtatagtta tttattcgca ggagtaccag tgcggtactc ctgttggttt taatgatcga
     2401 ggacgatcag tggtgaatgg ttggcaggat ctttaagtac ggtaatatcc gcgccgtaga
     2461 gcattgtcag cgcctgctgt gtcaatacgt cctgcggctt accatttgcg ataacctttc
     2521 ctttttgcat taatacaaca cggtctgcat aacgcgcggc aagatttaga tcgtgcaata
     2581 cgcagcaaac attgaactgc cgttcgtgaa ctaactggcg taataaacga aacaagtgct
     2641 gctgatggtg aatatccagc gccgaagttg gttcatcaag aaataaccat ttcggtgagg
     2701 gagtaggttc ccagagttgc accagcaggc gcgctaattg cacgcgctgt tgttcaccac
     2761 cggataattg ccgatagtcg cggtttgcta acgcctgaca gtcacataac gccattattt
     2821 gtgctgtttc attatcctga ttaccagtac gatgcggatg ccgccccatc tggataactt
     2881 cttgtacgct gaatggaaac gccatgtggc tattttgacg catcacggcc cggtgtttcg
     2941 ccagttcagt aatcgaccat tcatttaatg gcttattaaa caaccgacat tcgccgctat
     3001 caggttgaag atagccggtt agctggcgaa gcagcgtaga ttttcccgca ccattagggc
     3061 cgaggatggc aacaatttcc ccaccgggaa aagtcagcga gacattatca gtcagacgcc
     3121 gcccctgaag gctataaacc agattctggg cgctgatcat gtgatcctcc ggttgcgcag
     3181 aatcaaccac ataaaataag ggccaccaag caggctggtt aataatccca cgggcatttc
     3241 tgctggctgt accagcgtgc gggcaagcgt gtctgccatc agcaataaac aggctccggc
     3301 gagggcggaa caagggatta gccagcggtg atttgccccg gtagtcatgc gaatcagatg
     3361 cgggatcacc aggccaataa agccgataat accgcttacc gatacggcag caccaacgag
     3421 cagtgagctc actaacagta attgctggcg tttacgctta acgttcacgc cgaggtagtg
     3481 ggcttcttca tcaccgagct gcagtaaatt cagcgttccg gcgagacaag ttgttgcgat
     3541 aatggccggt aggatgaatg aactggcaac caataacgtc ggccattgcg cctgtccaag
     3601 attgcccatc atccacaatg tgagttgtcg tagctgctgc tcatcgccga tatagctcag
     3661 gatgccgatc gctgcgccgc acagggcatt gatggcgata ccggcgagta ataattgcat
     3721 catgctgcca tcgcgatggc gctgcgtgat gagaaaaatg atggtgcaga ccactaaact
     3781 tccggcaatg gcaaacacca tttgctcata gagcaccagc accactggga aggagaaggg
     3841 cagcacaata gcaacgccaa ccattaatgc agaaccgcta ctgacgccaa gtaatccagg
     3901 gtcagccatt gggttgcgaa aaagcccttg cataattacc cctgcggttg ccaacgctgc
     3961 acctaccagc accgccagca aaacccgtgg tagacgaata ttgagccaga tatcgcggta
     4021 ttcctcatca aaaagggcat caaaactgat ctttaacgct ccctgactgg caccaaacag
     4081 caccagagct agcaataata gtgatagccc cataaagacc ctggcggaag agagtgaatc
     4141 cttgagcacg caaaactccg ttacgacatt acggcgcgga catgccgcgc cgggggatca
     4201 cgccggtttc aggtccggtt caatcagact gtagacctgc tggttaagtg ctggcgcatt
     4261 tgccagttca tggatatgtg cggcgacatc agcacggtta acaaaaccat gacactcctg
     4321 attttgtatc cgttgtgctt tgcccgttgc cgcaccatca agcagaccgc cagggcggag
     4381 aatggcataa tcaagctggc tggtttgcaa ccagctttcc gccagcgttt tttcccgtac
     4441 cgcctgacca aacgcagcct ttgcccgctc tgataaaaac ggccagctat cgccgcatcc
     4501 caaagaggtc accagaatca tgcgactgat gccagccttc tcggcctcat caatcactgt
     4561 tctgtgcgcc agataatcct gagctccgcc cattgttgag atgataagcg catccgggcc
     4621 agctgcgcgg caagcagccg cgactacgct ggcatcacag gcgtcgcctg taaaaacctg
     4681 tacgccttgt tgcgccagtt tcgtggcggc atcggcatga cgaatgacag caacgaccgg
     4741 acgttgttcc gccagcgcca gttcaagcgt tctggcaccc acaccttttc cgcccgcacc
     4801 aaagagtagc cacggtgtca tcaggcgtgt tcctttaacg atgctgccag agcatggaag
     4861 gcgctgactt gttcgctcag gagctgacga tgatcatccc taccgaggaa gattttcagc
     4921 attgcgctgc cttccttatt gaaaaagaga atagaggcgg tgtccatgcc cataaattta
     4981 cgttcgatca aggcaatatg cgtgcagttt tctgctttga tatgcccgga catgccgtgc
     5041 tttccgcgta ggttgaaata gccgtggcga tggaaacccg aaggcagttc gccgctaaat
     5101 tcgagtatta catcggtagt atgtactaac gtggtgacgt tgccccattc gcagacggta
     5161 tcccagacag tatcgaattt atcacctgac acaacagtcg gagagggcag atttttcact
     5221 acctccagca gcgtggtgtt gtattgttcg gcgatcgcct ccagcgtacc gtctggctcc
     5281 gttttcaaaa attcctgtaa cgagacatgg ctcataaatc actcctgtta tggtttttca
     5341 agcgcaattg ctggcgcatt aagtacctga atcagttcat taagagactg caaaatgttg
     5401 cttgcccaga aacgaccttc attggtcaac cgcagacacg ttgacgcatc gcggcttaag
     5461 cctttttggt gccactgggc cagtaacggc gcgagttttt cggcatgtgg cgtaagttcg
     5521 tccagcggta cacgcgccgt ttcaatgccc gactgcaagg tatgacgcca ctgcgcgtta
     5581 cgttcggcgt tacgcatgat catcatcagc ggttttttgc ctgctgcgac ggattcatgc
     5641 caggtctgaa gattgcgttc gttcatccag gagtaaccat taatcgaacc gccggctccg
     5701 gaaccaaagg ccagacaatc ggcaccttgt ttaatcagca ggttatagag attgcgttcg
     5761 cgtgtggtac ggccccagtg gctgttactg atgcagcgcc aaccggcatc gtccataaaa
     5821 tcacaccctt gcaggtaaag atcgcgacgt tctgccggag agggcacggt agtacgccca
     5881 ttttccacgg ctttgcccag cggtgtattg ggcaggacat tgagcgcata gagatcgacg
     5941 ccgtcgagac cgatatcgcg ggcaatagcc agatcttctc cccaggtttg cgcatcctga
     6001 cccggcagac caaacagcag gtcacagacc accgcggcac ggtcgcgttt gaccaggctt
     6061 tccataaacg caatagcggt tgggccatcc gaggtgcggg ccattttctt gcggattttg
     6121 ctgttaaacg actgaatacc aattgagaag cggtttgctc cagcatcaag gcaagcatcg
     6181 attcgctcag cgtcaaaatt cagtacccgg ccttcgatag tgatttcaca atcaggggcc
     6241 agtggcaact tttctcgtaa tgtggtgatg attctggcca aatcgtgtgc tgaaagcgcg
     6301 gaaggcgtac cgccaccgaa atagaccgcg tggatggggc ccgactgatg caatacgctg
     6361 tctgcttcca gttctatttc acgaatcagg gcgtcggtat aatgagcaca cacatcttca
     6421 ttaaaacggt tctgataaaa accacaaaat gtgcagtgtg tcgcacaaaa aggaatgtgt
     6481 aggtaaacca gtcgcttacg tggtgatacc gtttgattga tcatctcttg ccaggtctgt
     6541 accagttgct ctttggcaac cggaatggcc ccacgaaacg gcatcatggc gcgcctgtct
     6601 ttaaagggct gatcaccgtc taaagcaaaa tgtggcgtga gatccagggt gttatttgtg
     6661 ttcattcgtc tgatttaaat ccatttattg cggccaaagc tgctggtgta aagactcaac
     6721 gacatcagca atacgcggac ccattcccag aatcaggttt tgatcaacgg tgataatgcg
     6781 ctggtttttc catgcggcag tgtgggtaat tccggcaata gaacgtagcc gattaatatc
     6841 gccatcgacc atttgtgagg tgacgacaat cacttcaggg ttagccgcaa tcagcgattc
     6901 cgcgctgtag cttttgtact gctggtgggt agcgacgttt tctgctccgg caagcgaaag
     6961 aatggcgtcc gcgacgctgc ctttaccggc aacctgtggc gcacttccgc ctgcggagag
     7021 aataaacatg gctttaaccg gggctttttt ggccgccacg ttttgctgta ctcgctccag
     7081 gcgttggttg atctgtgtca ccagcgcctc gccttgttca ggaacctgta acgtttttgc
     7141 cagttggcga atgttggcgt acatctgctc aagggtggct ggtacacgcg gcagagtgac
     7201 gacgttgact ttttgcgccc gcagttggtc gagcacaatt tgcggtcctg catcctgcca
     7261 ggtaattacg ctatccgggc gaagtgacaa aatgccttcg ctgctgagtt gtttccagta
     7321 accaatatgc ggcagtttgg cggtttctgg tggataggat gtcgtttcat cgacaccaac
     7381 cacgcgtttg ccagcgccca tcgcgtagat tagctccgtc agcgatcctc ctgcgaccac
     7441 gatacgttcg gcagccgtta cgctaaaagt ccagctaaga gccagtaatg ccgtaaacaa
     7501 tttttttcgt tttaccatgg tcttcgcggc agagaccacg gctgaaatac ataacgttag
     7561 tggggaaaaa agaaacggcc tggtgatgat ccttggcata atgtgagaac tcttatcaaa
     7621 ttgagaatga tcatgattat catttgagtt atatatgaaa tacaaccgtc gattcaatac
     7681 gcacggcgtt acaagcgtat ttatttttga tttttatcaa gatgcttcgt cagctaatgt
     7741 tcttgttacc gattaaattt cagcgcggga aaaagctgag aacccggaga tatcgcggct
     7801 tgcagagcaa agagaagaga aaattatcac tgttaacgac aacgttttgt gatgaggttg
     7861 tggggggata gccataaaca caggatggtt ttctggatgc tggatagatt tcataacccc
     7921 ctgtttattg tgttaatggc ggttaaaaaa agcttgatca taaaaaattg atcgataata
     7981 attctcatta tcacgtttga gtttaacaaa atatgacatc atggataaca aaatcatatc
     8041 ttttgtaagg gaaaacgcac agcactcttc acttcagacg tggcgtacta ctttgcctga
     8101 caaagagggc gtgcttttgc tgaattatca gaaatattcg gcaattttac gggatatata
     8161 cgctaatagc ttcccgtggt gatatctaat catttgaatt gtaaataaat ttatttttcg
     8221 ccagccaccc cggtattaac ccaggcagcc agcatttttt attttcctca tggagaatcg
     8281 ctatgtcacg tccgcaattt acttcgttgc gtttgagttt gttggctttg gctgtttctg
     8341 ccaccttgcc aacgtttgct tttgctactg aaaccatgac cgttacggca acggggaatg
     8401 cacgtagttc cttcgaagcg cctatgatgg tcagcgttat cgacacttcc gctcctgaaa
     8461 atcaaactgc tacttcagcc actgatttgc tgcgtcatgt tcctggaatt actcttgatg
     8521 gtaccggacg aaccaacggt caggatgtaa atatgcgtgg ctatgatcat cgcggcgtgc
     8581 tggttcttgt cgatggtgtt cgccagggaa cggataccgg acacctgaat ggcacttttc
     8641 tcgatccggc gctgatcaag cgtgttgaga ttgttcgcgg accttcagca ttactgtatg
     8701 gcagtggcgc gctgggtgga gtgatctcct acgatacggt cgatgcaaaa gatttattgc
     8761 aggaaggaca aagcagtggt tttcgtgtct ttggtactgg cggcacgggg gaccatagcc
     8821 tgggattagg cgcgagcgcg tttgggcgaa ctgaaaatct ggatggtatt gtggcctggt
     8881 ccagtcgcga tcggggtgat ttacgccaga gcaatggtga aaccgcgccg aatgacgaat
     8941 ccattaataa catgctggcg aaagggacct ggcaaattga ttcagcccag tctctgagcg
     9001 gtttagtgcg ttactacaac aacgacgcgc gtgaaccaaa aaatccgcag actgttgaag
     9061 cttctgatag cagcaacccg atggtcgatc gttcaacaat tcaacgcgat gcgcagcttt
     9121 cttataaact cgccccgcag ggtaacgact ggttaaatgc agatgcaaaa atatactggt
     9181 cggaagtccg tattaatgcg caaaacacgg ggagttcagg cgagtatcgt gaacagataa
     9241 caaaaggagc aaggctggag aaccgttcca ctctatttgc cgacagtttc gcttctcact
     9301 tactgacata tggcggtgag tattatcgtc aggaacaaca tccgggtggc gcgacgacgg
     9361 gcttcccgca agcaaaaatc gattttagct ctggctggct acaagatgag atcaccttac
     9421 gcgatctgcc gattaccctg cttggcggaa cccgctatga cagttatcgc ggtagcagcg
     9481 acggctacaa agatgttgat gccgacaaat ggtcatctcg tgcggggatg actatcaacc
     9541 cgaccaactg gctgatgtta tttggctcat atgctcaggc attccgcgcc ccgacgatgg
     9601 gcgaaatgta taatgattct aaacacttct cgattggtcg cttctatacc aactattggg
     9661 tgccaaaccc gaacttacgt ccggaaacta acgaaactca ggagtacggt tttgggctac
     9721 gttttgatga cctgatgttg tccaatgatg ctctggaatt taaagccagc tactttgata
     9781 ccaaagcgaa agattatatc tccacgaccg tcgatttcgc ggcggcgaca actatgtcgt
     9841 ataacgtccc gaacgccaaa atctggggct gggatgtgat gacgaaatat accactgatc
     9901 tgtttagcct tgatgtggcc tataaccgta cccgcggcaa agacaccgat accggggaat
     9961 atatctccag cattaacccg gataccgtta ccagtaccct gaatattccg atcgctcaca
    10021 gtggcttctc tgttggttgg gtcggtacgt ttgccgatcg ctcaacacat atcagcagca
    10081 gctacagcaa acaacctggc tatggtgtga atgatttcta cgtcagttat caaggacaac
    10141 aggcgctcaa aggcatgacc actactctgg tattgggcaa cgccttcgat aaagagtact
    10201 ggtcgccgca aggcatccca caggatggtc gtaacggaaa aattttcgtg agttatcaat
    10261 ggtaatcatc tgccccgata tttcggggca tttatctgga aggaagagag acaatgaacc
    10321 actacacacg ctggcttgag ttaaaagaac aaaatcccgg aaagtacgcg cgtgacatcg
    10381 caggtttaat gaatatcagc gaagcagaac tggcatttgc gcgcgtcacg cacgacgcgt
    10441 ggcggatgcg cggcgatatt cgtgaaattc tggcggcgct cgaaagtgtt ggcgaaacca
    10501 aatgtatttg ccgtaatgaa tatgcagtcc atgagcaagt tggtgcgttc acaaaccagc
    10561 atttgaacgg acatgccgga ttgatcctca atccgcgcgc gctggattta cgtctgtttc
    10621 tcaatcaatg ggccagtgtt ttccacatca aagaaaacac ggcccgtggc gaacgccaga
    10681 gtattcagtt ctttgatcat cagggcgatg cattactaaa agtttatgcc accgacaata
    10741 ccgatatggc ggcatggagt gagcttctgg cacggtttat caccgatgag aatacgccgc
    10801 ttgagttaaa agccgttgat gcgccagttg ttcaaacgcg agccgatgcc agtgtggtcg
    10861 agcaagagtg gcgggcgatg accgacgttc atcagttttt tacgttgctc aagcgtcaca
    10921 atctgacgcg ccaacaggcg ttcaatctgg tggcagacga tttggcctgc aaagtatcca
    10981 acagtgcgtt ggcgcaaatt cttgaatctg cacagcagga tggcaatgaa atcatggtgt
    11041 ttgttggcaa ccgtggctgc gtacagattt tcaccggtgt ggtagaaaaa gtggtgccaa
    11101 tgaaaggttg gctgaatatt ttcaacccga cgtttactct tcatctatta gaagagagca
    11161 ttgctgaagc ctgggttacc cgtaaaccga ccagcgatgg tcacgtaacc agtctggaat
    11221 tgtttgccca tgatggtacg cagatagcgc aactttatgg tcaacgtaca gaaggtgaac
    11281 aggagcaagc gcaatggcgt aagcaaattg cttcgttgat accggaaggc gttactgcat
    11341 aacgtataat taacgggagg tctggctgtt gctgggcctc tttatattga tcaatcgttc
    11401 acaccagata attaatatgc tgatggcgta ccagctcatt gatccgctta agattgagga
    11461 tcatcatgat attgtgttta tggcagtaga acgtttttag cgatattttc agtttctcca
    11521 gaatatcttc cgttgtcatt tgattcgcaa tacagctcag aacctgatgc tgcttgcgcg
    11581 agagggacac atccgcttca ggcacagagc agtaactgcc attgatgagt gcgacaaagt
    11641 cgatgatggc gatagttcgc ggaacaaagg tcaccggtac tttccccagg cagcgtttaa
    11701 tattaaaggg ggagaaaata attacatgga caggttttaa tcgttcaatt tgagtgagaa
    11761 aattagaatg aaatacatta ttcattaatg tatcaataat gacgaaggca ttctgatgca
    11821 acattacgtc gatctcttct tcgttctgta tatgaacgac attaccttta ctcagaatgt
    11881 ttttcatcgc ggtgaaaaac atcgtatccc tggtaattat aagaaacata accgactcct
    11941 ggtacggaca ttaactttat aatcagtttg ccaaaggtaa ttaacaggac agggcttata
    12001 ataatagtta gcttactgtg tattaatttg atgtaatgca tgatgcagat gctgagaaag
    12061 catctgcatc tttcggtggt gttatttgac cagctcaggt gttacctgac tcaccgcatt
    12121 ggtgtagtaa ggagcccccc agcccggttc cggccagaat gcaccatagc cgtaatccca
    12181 caggttatag gtggtattaa ccacttctct caaatgccac acctggatgc cctgcatatt
    12241 cacttcgagg aagttatacg ggactttatt gataaagcca ggttgttcac cctgaatggt
    12301 accgaggatg gtaacaaagt gattacgata gttcactgga tcaaggaagc cgctttgtct
    12361 ggcgagcagt cggccctgat agttggcttc aatatcaggc ttcgcatagc tatccaacgg
    12421 taatacagcg atttctaaca acgtatccgt tttgccattg ataacgttga taaccttccc
    12481 accaaagcgc gcttgttgac caacatataa ccccggctgg ttatgaacag caacaaaact
    12541 tttttgaata tcaggttggt tattgccttt gatattttgc ggaattgaac tacatgcggc
    12601 aagcaaaaat gaaaggctga ggatgagtgc accttttgtc atgttcatgt tactatcctt
    12661 atcaacaaat attaaaacca taatagatat ttctttatag ttttcatctg attctgaggc
    12721 gatatgcctg atagtaacgt aaacctgtct gatgtttata tctcttctgt ttagttgagc
    12781 tcagttaatt tttgggcgtg gcatgagtat ccattgttcc ataatttata ttaggaggtt
    12841 tttaatcttt tcgtgtctaa tgttatttat ctgattaagt attgattgtg tcttttgcgg
    12901 aagtggtgtc gagcttatcg tttcaatgtt attaataaga cttacaagtt gagcctcaac
    12961 tttgagtagt ctgcatttgt atatgtggtg ttcgtatggc acgcattaat ttgttggcgt
    13021 tgttgttgcg ctaaatcaaa ttgtttagag gtgtatcctt ctccagagaa tggggaatta
    13081 atgggttgtt gttcttcccg gttatcacaa ggccacttcg gtgtgtctgg cgttttaaca
    13141 ttatcggtat ttgttgtgca gttataaata tcaccgttat atgtatttat tacacctggg
    13201 gtaaagtcaa ttgacataac attccttatt catcatttgt tttatgctaa gattttaaaa
    13261 ttggataatt agaacaggaa tggattttct tctacataca gtaaatataa tgcgtcatat
    13321 cttcctgata taattacctt tgacttctct tttatttcca tctggtaata tttgagtgca
    13381 aaaaaaggca ataataatat cgcaaaagaa aacgttaaca actatcgaaa atgctcgttt
    13441 taaaaaggta tgatgttctc tgatagtgtg tgaagtgata tttttgtgat gttttagata
    13501 tgtaataata aaatgggtta acgtatgttg aaattattta ttgtgcaaac cactgataga
    13561 tcataatcca gtacatcttt acagtgctgc actgtatcgc tgataactaa gcagctcact
    13621 gatacaccat gctggacgcg ataattggaa tctcacgcag ggagtactgt gtaaatagca
    13681 aaatcgcctg atgcgctacg actgtcaggc ctacaaataa catacaattc atcgaattat
    13741 cataacgttg taggtcggat aagacgcgtc agcgtcgcat ccgacataaa taaagcgcat
    13801 ttttctaaca acctgtgggg gaatatagcc gctttatttc aggcgattac ccgcctcatc
    13861 aaccactttc tcgccatctt ccttggtgaa cgcgcctttt tgcgcatctg gcagaatttc
    13921 cagcacgact tctgaagggc ggcacaggcg agttccctgt ggcgtcacca caatcgggcg
    13981 attaatcaga atcgggtgct gaagcataaa gtcgattaac tgatcgtgag taaatttatc
    14041 ttctgcaagg cccagttcct catacggctc gacgttttta cgcagcaacg cgcgtacggt
    14101 aatccccata tcggcaatga gttttaccag ctcatcgcgc gtcggtggtg tttccagata
    14161 atagataatg gtcggttctg taccgctgtt gcggatcatc tccagcgcat tacgcgacgt
    14221 accgcaggcc gggttgtgat aaatggtaat gttgctcata tcagtatctc attacaatgt
    14281 gaaagagagg tgtagcgcca gcgccaccag cgttacaaac caaagcggac aagtcaaaaa
    14341 cacatatggt ttaacgaata tgtaagtgta gagaaaggtg taggtgtgaa ttaagcgttg
    14401 ggcattcatt tgaaaggagg tctgaatcag gacgtaaaaa aaccgacttc gcgtcggttt
    14461 ttttactgtt cagccctgag ttggtggctc tggctggagt gagatagccc acatctaaca
    14521 atgagacatg ctccgtgaca ggcaatggat gagtaagcgg atgcattctc actccatcgc
    14581 atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa ttgcgcagct
    14641 ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta gctaagggct
    14701 aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact cttctgctgc
    14761 tgtcgggtga atggcaaccg tgttgtcgaa gtcttttttg gttgcaccca tcttcagcgc
    14821 caccgcgaag ccctgcaaca tttcgtccat accaaaacca atgccgtgaa taccgacaat
    14881 cttctcttcc ggtccaatgc ataccagttt catgcgacac ggctggcggt gagtggtgac
    14941 ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt cgccgtactg
    15001 ctcgcgcgcc tgcggctccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac
    15061 ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca ggcgttcaga
    15121 gagacggcga cctgctgcaa ctgccaccgg agtcagttcc actgcacccg tgttatcgcc
    15181 caccgcgtaa ataccttcaa cgttggtgtt ttgatattta tcgacgacga tatagccttt
    15241 ttcgttggtt ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc
    15301 aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct ccagcgtcag
    15361 gctgccgtcg gcatttttca ccaccgcttt cgggatggcg ttggtatgca actgtgggcc
    15421 ttcggcgttc atcacttcga ccagcgtttc agagatcatc gggtcgaagc tgcgcagcgg
    15481 cgcatgttta cgcacaaaca gatgcgtctt cgcaccgagg ccgttaatca cgcccgccag
    15541 ttcaacggcg atgtaacccg cgccaacaac cgctacgcgc tctggcaaag ccggaagggc
    15601 gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg
    15661 accaccggtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttga cttcaatggt
    15721 tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac
    15781 gttctcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaatgtttc
    15841 ccagttgaat ttattgatag tggtatcaaa gccgtaatcc gggccgtaca tatggatcgc
    15901 ttcacggatt tgtgccgcgt gccacatcac ttttttcggc acacagccaa catttacgca
    15961 ggtgccgccc agctctttgg cttcaatcag tgcgcatttc tggccgtaca tagccgcgcg
    16021 gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt
    16081 catgacatat tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc
    16141 agcaatggct gcaattactc tggcacgatc cagcttacgg tggcgtgccc ggtgcctgct
    16201 ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgctgttc cagcttccac
    16261 ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagc
    16321 accgccagtt caatttgcag aattttgcga atgccggtcg cttccaggtc gtggatcatg
    16381 cgcttaatct gctggcgcag caccactgga taccacaatg cgtaagtgcc agtggcgaaa
    16441 cgtttgtaac cttctgctat cccgctgacc accgcctgat agtcggtttt catttcatac
    16501 ggcggatcga taaggattaa accacggcgg gaaaccggcg gtagtttggc cttaagctgc
    16561 tggaaaccgt cggctttttc aacgcgcgca cgattgtctt tctggaattc cgaacgtaac
    16621 aacggataat cgctcgggtg cagttcagtc agttgcaggc tgtcctgttc acgcagtagc
    16681 tggcgcgcaa tcaacggcga accggggtaa taacgcaact gcccgctacg gttgaagtgt
    16741 tttaccacat tgatgtacgc ctccagctcc gcaggcaaat cgtcctgctg ccagatacgg
    16801 gcgatacctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc
    16861 ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt cagcgactcg
    16921 atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg
    16981 tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg
    17041 gcgcacaaaa gtctatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac
    17101 gtttctcatt gaaattcact acacttaacc ccatgctaca aacattatgt aaagcgcctg
    17161 ttgagcgctt ccttaacctc tttaaccagg actgcgctaa tgacgaatcc gttactgact
    17221 ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact
    17281 aaggcgctga acgactgccg cgaaaatgtg gagcgcgtag tagcgcaagg ggcaccgtac
    17341 acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgctggg gcgtatcttc
    17401 tccccggtca gccatctgaa ctcggtgaaa aatagcccgg aactgcgtga agcgtacgaa
    17461 caaaccctgc cgctgctgtc agaatacagc acctgggtag gacaacatga agggctgtat
    17521 aaagcatatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggcgcagaaa
    17581 aaagcggttg ataacgcact gcgcgacttc gaactctctg gcatcggtct gccgaaagag
    17641 aaacagcagc gttacggcga aattgccact cgtctttctg aactgggcaa tcagtacagc
    17701 aacaacgtcc tcgatgcgac gatgggctgg accaaactcg ttaccgacga agcggagctg
    17761 gcggggatgc cagaaagcgc gctggctgcg gcaaaagccc aggccgaagc gaaagagctg
    17821 gaaggttatt tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac
    17881 aaccaggccc tgcgtgaaga gatgtatcgt gcttatagca ctcgtgcttc cgatcaaggt
    17941 ccgaacgccg gtaagtggga taacagcaag gtgatggaag agatcctcgc gctgcgtcac
    18001 gaactggcgc aactgctggg ctttgaaaac tacgccttca aatcacttgc cactaaaatg
    18061 gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgcccg
    18121 caaggcgaaa aagagctggc acaactgcgc gccttcgcca aagccgaatt tggcgtcgat
    18181 gagttgcaac cgtgggatat cgcttactac agcgaaaaac agaaacagca cctctacagc
    18241 atcagcgatg aacaactgcg tccgtacttc ccggaaaaca aagcggttaa cggcttgttt
    18301 gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg
    18361 catccggatg tacgtttctt cgaactatat gacgagaaca acgaactgcg cggtagcttc
    18421 tacctcgacc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta
    18481 ggccagatgc gtaaagccga cggttcgctg caaaaaccgg tcgcgtatct gacctgcaac
    18541 ttcaaccgcc cggtaaatgg taaaccggcg ctgtttaccc atgacgaagt gatcaccctg
    18601 ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcattgaaac cgctggtgtt
    18661 tccgggatca gtggggtgcc gtgggatgcg gtcgaactgc cgagccagtt tatggaaaac
    18721 tggtgctggg agccggaggc gctggcgttt atctccggtc actatgaaac cggcgagccg
    18781 ctgccgaaag agttgctgga taaaatgttg gcagcgaaaa attaccaggc ggcgctgttt
    18841 attctgcgcc agctggagtt cggcctgttc gatttccgcc tccatgccga gttccgtccg
    18901 gatcaggggg cgaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtggta
    18961 ccgtctccgt catggggccg tttcccgcat gctttcagcc acatttttgc cggtggttat
    19021 gcggcaggtt actacagcta cctgtgggcc gatgtactgg cggcagatgc cttctcgcgt
    19081 tttgaggaag agggcatttt caaccgtgaa actgggcagt cgttcctcga caacattctg
    19141 agccgtggcg gttcagaaga gccgatggag ctgttcaaac gcttccgtgg tcgtgaacca
    19201 cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag tgaaaatctg
    19261 cttaattgat gaaacaggcg ccggagacgg cgccttatct gttctggcgg cccgctgggg
    19321 gctggaacac gatgaagaca acctgatggc gctggtgtta acgccggagc atctggaact
    19381 gcgcaagcgt gatgagccga aacttggcgg catctttgtt gattttgttg gcggagcgat
    19441 ggcgcaccga cgcaaattcg gcggtggtcg cggagaggcg gtggcgaaag cggtgggcat
    19501 taaaggcgat tatttgccgg atgtggtgga cgccaccgca gggctggggc gcgatgcctt
    19561 tgtgctggct tcggtcggct gccgcgtgcg gatgctggag cgtaatccag tggttgccgc
    19621 gttgctcgac gatggtctgg cgcgtggtta tgcggatgcg gaaatcggcg gctggttaca
    19681 ggaacgtttg cagttgattc acgcctccag tctgacggcg ctgagcgata tcaccccgcg
    19741 tccgcaggta gtttatcttg acccaatgtt cccgcataag cagaaaagcg cgctggtgaa
    19801 aaaagagatg cgcgtgtttc agtcgctggt ggggccggat cttgatgccg atggattgct
    19861 ggagcctgcg cagttgctgg caaccaaacg cgtggtggta aagcgtccgg actatgcgcc
    19921 gccgctggcg aatgtcgcca cgccaaacgc ggtagtcact aaagggcatc gctttgatat
    19981 ttatgccgga acgccggt
//
LOCUS       DAOFSV010000079        19841 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.079, whole genome shotgun sequence.
ACCESSION   DAOFSV010000079 DAOFSV010000000
VERSION     DAOFSV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.079"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(2..2182)
                     /gene="katG"
                     /locus_tag="QBH27_002925"
     CDS             complement(2..2182)
                     /gene="katG"
                     /locus_tag="QBH27_002925"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDQ7015212.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(2511..3401)
                     /gene="metF"
                     /locus_tag="QBH27_002926"
     CDS             complement(2511..3401)
                     /gene="metF"
                     /locus_tag="QBH27_002926"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDQ7015213.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(3783..5336)
                     /locus_tag="QBH27_002927"
     CDS             complement(3783..5336)
                     /locus_tag="QBH27_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDQ7015214.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELMGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSNRPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            5469..6599
                     /locus_tag="QBH27_002928"
     CDS             5469..6599
                     /locus_tag="QBH27_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDQ7015215.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTETICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITTPEIIETVALLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHDNNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGAI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGKTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCASRLISD"
     gene            complement(6563..8995)
                     /gene="metL"
                     /locus_tag="QBH27_002929"
     CDS             complement(6563..8995)
                     /gene="metL"
                     /locus_tag="QBH27_002929"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDQ7015216.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(8998..10158)
                     /gene="metB"
                     /locus_tag="QBH27_002930"
     CDS             complement(8998..10158)
                     /gene="metB"
                     /locus_tag="QBH27_002930"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDQ7015217.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            10435..10752
                     /gene="metJ"
                     /locus_tag="QBH27_002931"
     CDS             10435..10752
                     /gene="metJ"
                     /locus_tag="QBH27_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDQ7015218.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            10812..11420
                     /locus_tag="QBH27_002932"
     CDS             10812..11420
                     /locus_tag="QBH27_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDQ7015219.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEDSKYIVRRVKGELNAEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11481..11693)
                     /gene="rpmE"
                     /locus_tag="QBH27_002933"
     CDS             complement(11481..11693)
                     /gene="rpmE"
                     /locus_tag="QBH27_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDQ7015220.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            11896..14094
                     /gene="priA"
                     /locus_tag="QBH27_002934"
     CDS             11896..14094
                     /gene="priA"
                     /locus_tag="QBH27_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDQ7015221.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            14250..15275
                     /gene="cytR"
                     /locus_tag="QBH27_002935"
     CDS             14250..15275
                     /gene="cytR"
                     /locus_tag="QBH27_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDQ7015222.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            15367..16326
                     /gene="ftsN"
                     /locus_tag="QBH27_002936"
     CDS             15367..16326
                     /gene="ftsN"
                     /locus_tag="QBH27_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDQ7015223.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            16419..16949
                     /gene="hslV"
                     /locus_tag="QBH27_002937"
     CDS             16419..16949
                     /gene="hslV"
                     /locus_tag="QBH27_002937"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDQ7015224.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            16959..18290
                     /gene="hslU"
                     /locus_tag="QBH27_002938"
     CDS             16959..18290
                     /gene="hslU"
                     /locus_tag="QBH27_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDQ7015225.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            18357..19283
                     /gene="menA"
                     /locus_tag="QBH27_002939"
     CDS             18357..19283
                     /gene="menA"
                     /locus_tag="QBH27_002939"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDQ7015226.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            19376..>19841
                     /gene="rraA"
                     /locus_tag="QBH27_002940"
     CDS             19376..>19841
                     /gene="rraA"
                     /locus_tag="QBH27_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDQ7015227.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSE"
BASE COUNT         4778 a         5308 c         5163 g         4592 t
ORIGIN      
        1 attacagcag gtcgaaacgg tcgaggttca tcactttcac ccacgctgcc acgaagtctt
       61 taacaaactt ctcgtgggca tcgctgctgg cgtagacttc cgccaccgca cgcaggacag
      121 agttagaacc aaagaccaga tccgcacggc tggctgtgta tttcacttcg ccagtttcgc
      181 ggtcacggcc ttcgaacagt tctttcgatt cgtcggtcgc tttccactcg taacgcatat
      241 ccagcaagtt cacgaagaag tcattgctca atacaccaac gcggtcagtg aagacgccgt
      301 ttttgctgcc atcgaagttg gcacccagta cgcgcatccc gcccaccagc gcagtcattt
      361 ccggcgcggt cagcgtcagt tgctgtgctt tatcaatcaa cagtgactcg gtggtggaaa
      421 cgtccagacg agcgcgatag ttacggaaac cgtcagcaat tggttccagc agttcgaaca
      481 tctcaatgtc agtctgatcc tgacgcgcat caacgcgacc cggcgcaaac ggtacatgaa
      541 tgctcaaacc tgcggcgctt gcggctttct caacgccaac cacacctgcc agcacgatga
      601 tatccgccag cgaagctttg ccagactctt tctgaatttt ctccagaact ggcagagcac
      661 gaacggctgc ggcgttcaca tcccagtcac gctgcggcat taatgccaga cgcgcaccgt
      721 tggcaccacc acgtttgtcg ccaccacgga aggtagaagc agacgcccag gccaccgata
      781 ccagctcact aacagacaga ccagaatccg caatcgcgaa tttcagatcg ataatgtcct
      841 gctcggtcgg gttgtagatc ggctgcggca gcggatcttg ccagatcaga tcttctttcg
      901 gtacttccgg cccgatgtaa cgagatttcg gccccatatc cctgtgcgtc agtttgaacc
      961 aggcacgagc aaaggcttcg ttgaacgcct gcggatcgtt gaggaaacga cgagagatct
     1021 tctcgaactc aggatcaaaa cgcagcgtca ggtcggtcac cagcatcgtc ggtttacgtt
     1081 tcttcgacgg atcaaacgga tccgggataa tttccggtgc gtctaccgct tcaaactgga
     1141 ttgcgccagc cgggctgcgg gtctgtaccc actcatactt gaacaggttc tcgaagaaat
     1201 agttgctcca ctgggtcggc gtctgggtcc agaccacttc cagaccagag gtaatggcat
     1261 ctgcgccaac gccgctgccg taagtgctcg cccagcctaa accttgttct tcaatcggtg
     1321 cagcttctgg atcaggacct acatttgatg tcggaccggc accgtgggtt ttacccagcg
     1381 tatgaccacc cgcaatcagc gccacggttt cttcgtcatt catgcccatg ttgccgaagg
     1441 tcgcgcggat agctgctgct gccgaaagtg gttcgccgct gtgatccggg ccttccgggt
     1501 taacgtaaat caggcccatc tcggttgcac ccagcggtgc tttcgccagc gcttccggat
     1561 gacggtgagt cagccaggct ttttcatcac cccagttaac atccagatcc ggttcccaga
     1621 cgtcttcacg accggcacca aaaccgaagg tacggaagcc ggagttttcc agcgccacgt
     1681 tacccgcgag gataaacagg tcggcccagg agattttctg accatatttc tgtttgattg
     1741 gccacaacag gcgacgcgct ttatcgaggc ttacgttatc cggccaggag ttcagcggtg
     1801 caaagcgttg ctgaccacga cccgcgccgc cgcgaccgtc gattgaacgg taagtacccg
     1861 cgccgtgcca ggccatacga ataaacagac cagcgtagct gccccagtcg gcaggccacc
     1921 acggctgaga ttctgtcagc agggctttca gatctttttt caggccgtag taatctaatt
     1981 tgctgaattc tttgcggtag tcaaagtcct cacccagcgg gttagaacga ttagaatgtt
     2041 ggtttaacag gtcaacacgg agttgatttg gccaccagtc gcgcgtggtt gtgcccccac
     2101 ctgcgctctg gtcgtgaccg ccctgatgga acgggcattt gccggtggct gtcgtgttat
     2161 ggatatcgtc tgacgtgctc atcaatgtgc tcccctctac agtgttaccg ttacgataca
     2221 cagcgttaga gagaagttat aattgaatta atccacggat gcgatagttg agatcttaca
     2281 tattgttggt taaagagatg tagatcaaat tgatcgtaat taagactttt tgattatgtc
     2341 cgatttagga caaatgcttt ataaaaaagg gttttagatt tgtatccttc aagtaatgag
     2401 ggagaaaaca aggctgataa ccttattttc ccgccctcat ttcgaggcag cattttgtgc
     2461 tctgtttaaa atttgtgaac actgtgtgat tttcacaaaa ggcacaatac ttataaacca
     2521 ggtcgaactc ccagtgtatg gcaaatcgca taactcattt cagcgcggtt aagcgtatag
     2581 aagtggaaat ctttcactcc ttcacggctt aaaatcttca ccatatccat ggcgatattc
     2641 gcgcccacca gcttgcgggt ttcagcatcg tcatccaggc cgtcgaacat ttgtgccatc
     2701 cacgccggaa tacgcacgtt ggtcatgtcg gcaaatttct tcgcctgttt aaagttcgaa
     2761 accggcaaaa ttcccggaat gatttccaca tcaatgcctg ccgagacgca gcgatcgcga
     2821 aaacgcaggt agctttcgac atcgaagaag aactgagtaa tggcgcggtt ggctccggca
     2881 tccacttttc gtctcaggtt gagtaaatcg gcctgggcac ttttcgcttc tggatgtact
     2941 tccggatacg ccgccacgga gatatcgaaa tctgccactt cttttaacag cgtcaccagg
     3001 tcagaagcat acatttctgg cttaccactt cccggcggca ggtcgccacg cagcgccacg
     3061 atatggcgaa taccgttatt ccagtagtcg cgtgcaatga tccgcagctc ggcaggcgtc
     3121 gcatcaatgc aggtaagatg cggtgccgct tccaggccgg tgcgatcttt aatgccttta
     3181 ataatgctgt gcgtacggtc gcgctcgccg gagtttgcgc cataggtcac cgatacaaac
     3241 ttcggtttca ggctgctaag gcgatcgatg gagttccaca gggtctgctc catttcactg
     3301 gtacgcggcg ggaaaaattc gaacgaaacg ttaatctgcc cctggacttc tgccagactc
     3361 tgattcaggg catcccgctg gctggcgtga aaaaagctca taccttacct catcaatcgc
     3421 ttatcgttat atgttgtgtt gtgcacatct atccgtttag acgtccagat gtaaagatga
     3481 aggaaaagcc gaagggcgtc aaccgaaaaa taaccaatga tggtgaggaa aaatcaggga
     3541 agatgaaaaa aactcatctt aattagaggc gataaaagat ttctctatag cgcaatccac
     3601 ctcgcacgtt ttgactcata ttctgacaac acagcgacac ctgggctaat tcgaaaaaca
     3661 acgtcctgcc accggatggt gatgttaagg catctgattc ggcctgcgcc ctcgcgtagg
     3721 ccgaatacag gatctgcgca atcaacgggt aacaaacagt tgagtcaatt cagtgcgcac
     3781 ttttatttga tatccttcac gcgcataccg ttgagctgtt catccgtaat ggtgttgccc
     3841 gctttgaagt aatcaaccac cgcgtgataa acgtaataac cgcccgttgt gttacgcgct
     3901 ttcccttcgg taaaggcggt aaaaccatct ccaccatcag caaggaaact ctgagtagca
     3961 atgtgataaa ccgtcgcatc ttcaatgggt ttgccattca aagtcagtgt gattacccgc
     4021 tgaccaaccg gcctgttgct gtcgtacttc atttccaggc ctttcgatac ctgtaaaacg
     4081 ccattgctta aacttgcgcc gtgttccatc agactgcgta attgtttacc catgagttcc
     4141 atcgtcacca gttcgttggg gaacggaaac gtactgatga cgccgcccat cgtgatcgcc
     4201 cctgcgggga tctcattgcg gataccacca gagtttgtta acgccagttg ggtgttttta
     4261 cccgccgcta ccaataaagc gtcagccgcc aggttcccga gggaagccga ttcaccatag
     4321 gcacgtttta attcaaccgg cgattgcgcc accgtttgtt gcaccacttc atcaagcttt
     4381 ttattccagc cgtcgatcac ctgtttcgtt tgcggatcgg gcttccactc gtcagcgtaa
     4441 atggttttaa gctcgaagtt tttcaccgta aaattatgcg gcttctcttt gtagtcgaga
     4501 accagtttgc ccacatcaat cccgccgctg tcagtggaga ggatgagcgt attgccgact
     4561 ttaatcggtt ccggcgtacc cacatgtgca tgtccggtga tcaaaatatc cagccctttc
     4621 acctgacttg ccgtctgaat atctttatcc aacgcgcgac gcacatctgt gcctcccata
     4681 ctggactggc gggctggaac accttcgtgg atcagggcga cggtcaaatc aaccttgcct
     4741 ttgagttcat cgatataacg ttgtagccat ttaatttcat cgcgcgcttc aatacccacg
     4801 cgcgtggcag cagataccgt atcattaaag gcaaatactc cgtgcaaacc aataacgcct
     4861 attttcacac cgtctttttc gatgatggta tagggcttat cccagaatga tttactgctg
     4921 ttctgataaa aaatattgcc ctgcacaata gggaattttg cctggctcaa ctgtaataat
     4981 gtgttgtccc agccgtggtc aaattcatgg ttgcctattg tgacggcatc aaacggcata
     5041 gtattcataa tatcgataat agccttacct ttggtcaggc tgctgatata tggcccggta
     5101 aaatagtcgc ccgcatcaaa aaaccatgtc gctttatttt ttgctttttc ttgcttaacc
     5161 agcgtagtga tatttgccca gccgccaata tcccgtttac cgtcagcgat ccacggcact
     5221 ttataaggct cgacatgtgc atggaggtcg ttggtataaa tgatggtgac atctttagcg
     5281 caagcccaga atggcagtgt taatgcgata cccgcagcaa gtattttaat tttcataaac
     5341 agtccctttc agtgagataa aggattgcta cattacgttc tgaaagactt atttatgtga
     5401 gagtaatcac atccgcaggg gatagttgcc ggacaaaatc aacgaccttg tgaaatcgtt
     5461 ggtgatatat gtcgactctg tttacgatta atgcctgtaa gtcgtttggt tgccgtaatt
     5521 tagggcagcc aacatcgaca gattatagct ggcccgacta tcgactgggt tatccggcac
     5581 tgcactgtcg ggcatgtggc agttacccgc cgttgtttga cgagcaacaa tttcgtgact
     5641 ggttgacagt tcacttatct acttttgcaa cagagaaagg ccatttttgc ccggtatgtt
     5701 atggcacaga gacgatttgt tatggtcaca atccacaggg ttcgcaacgc attcaatgcc
     5761 gtaactgcaa aaaagtatgg acacccaaac aatatcagaa agaaataacg actcccgaaa
     5821 taatcgaaac ggttgctttg ctcgtgccat ttcagggagt cagcagcggg cagaaactgt
     5881 atgttttaat cagttttgat gctcttcgcg gtaatattct tcatctttct actaattaca
     5941 cgcagcatca ggctggagaa agtctgcatt atcgatacag aggaaatgca gaaccagagt
     6001 tacacgacaa taatattgta caacgcgttg atatgcgcga agcacaattt ctacgccgca
     6061 gtcagtttga tgaaattcag tatggcagcg cagcgctcaa gcgcaatgcc aaaggcgcta
     6121 ttctgcgtcc ggttatcact gcacacgggc atttcagagt actgaatatt ttattcccta
     6181 cagtaaaaac acatgtaatt tcacacgagt gttttttaag aggtgccatt attacggcct
     6241 gggctgatct gtttcgccaa cagcaaggtg aaatttggtt tattgaagag gaaatagctg
     6301 acgataccga taatatgccc tggcgttttc agggaaaaac ttatcacggc tggtggaaaa
     6361 atcagtggca actctgggtt cagggaaaaa atcgtaaaat ggtatgtgca ttaacgggcg
     6421 gaaagagtag caaagcagaa atgctctctt tagcaaccag cagacatttt atcgactggc
     6481 tacataaaca agcagtattt actcacagcg ccccgctttc cgcagggcgc gttacgcaaa
     6541 tactgctttc tctggctcag gattacaaca actgcgccag tcggttaata tccgactgaa
     6601 tcgccccggc ggtgacgtcg cgccctgcac ctggcccgcg gatcaccagc gggttgtcgc
     6661 gataccagcg gctttcgatg gcaaagacgt tatcgcatgg cagtagtgat gccagcggat
     6721 ggtcttcacg caccgcttcc acgcccacac gcgctttgcc attggcatcg aaacgcgcca
     6781 cgtagcgcag caccagcccc atttcgcggg ccgcttccag tcgttgcacc atctgctcgt
     6841 taagttcatc gccattttca aagaaatggt cgatgctgcc gccttcgcaa tgggcaggca
     6901 ccagtgattc aacacgtacc tggtccggtt caatgttgta acccgcttca cgcgccagaa
     6961 tcaccagctt gcgcattacg tctttgccgg aaaggtcatc acgcgggtca ggttcggtta
     7021 agccttgctg ccacgcctga tccaccagtt cggtaaacgg cacgctaccg tcgaattgca
     7081 ggaacagcca ggagagcgtg ccggagaaga tcccactgat cgacaaaatg gtatcgccgc
     7141 tgtcgatcag atcgcgcacg gtatggttga tcggcaaacc cgcaccgacg gtggcgttgt
     7201 acagccagtg acgcccggtt ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt
     7261 cgcttgctcc tgccagtttg ttggcactga tgacgtggaa accgtggctg gcgaaatcaa
     7321 gatactggtc agcgagctgc tggctggcgg taacgtccag caccactaaa tcatcatacg
     7381 gatgggcgcg catccacagg aacagcgact cttcatcctg ctcgaccgct tcatcgttga
     7441 agaaggctaa cgcgcggctg gcgtccagac cgtcatagct caacaggcta cggcggctgt
     7501 ccaccacgcc tgccagcaca aattcaaagc cggtacgtgc cgaaagcgta ctctgctcac
     7561 gggcgaacag ttccagccag cgggaaccga tattgccctt gccgaacaat accaggccga
     7621 tgcgtttttc cgcgcggaag acggactgat gcagcccctg aatcaggctt tcggtcgggc
     7681 cggtgcgcag tactgccacc aggctgatgc cgtcatcgga ctgccaggta aattcgaccg
     7741 gctggccttt cagttgctgc cagaagcggt ggcaatgcag cgggttacgg gtgacgcccg
     7801 ccccaaccat cgccaccagc gccagcccct gacgcaggcg cagttcgcca ggtaatcccg
     7861 cttcgtcgag gattttcagc gcactgtcgg ccacttctga ggtgtagcaa aattgcagca
     7921 actggcgatc gttatgcacg ccaaccgcca gtggacgtac ctgagcacgt ttcaggatct
     7981 ggtcgatctc tttatgcgcc agtttgaaat cctggctggc gggcacctga aactcaatca
     8041 agcagacatc atcatggctg gtgacaatac gcgcaccagt accggaggcc agcacgcgtt
     8101 caatgcgcgt ggaaccttga tccggcgtgt agctacagcg cagttgcagg tcgatttcgc
     8161 tgccagaaac gggctgtaaa gtacgggcat gaagaacggg agccgccaga cgcgccagtt
     8221 cgctggcttc atccagacgc agcaacggca gcaggcaggc atctttcact ttacgcgggt
     8281 cggcgctata taccccggcg acgtcgctcc agatggttac gcgagaaaca cccgccagcg
     8341 caccgatttg tgtcgcggaa tagtcggaac cgttacgccc cagcagcacc gtttcaccgg
     8401 cgttgttacg gctgataaac ccggtcacca ccagacgttt gcccggatgt tgtaccagta
     8461 gctgttgcag caacgggtaa gaaagccctt catcaacctg cggttgcgca gcgcgttcag
     8521 cgcgtaaaaa ctcgcgggca tcaagccagg ccgatggcag cccttgttga ttaagcaccg
     8581 cagacatcag gcgcgccgac cacacttcac cgtggcccac cacttccgca tacaccgcat
     8641 cgttaatacc gctgtcgagc aacgccgcca ggcgctcaag atcgctgaca aaagcgctaa
     8701 tgaggttatc ggcttcttcg gcgggtagca ggccgctaat cagatcgcac tgataacgac
     8761 gtaatgtttg ttgaacctga tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt
     8821 taatcaactg gttagtggtg ctaccggcgg cggaaaccac catcatatcg tcaggctgag
     8881 agtactccgc cataatgccc gcgacacgca aataacactt cacatcagcc agactactgc
     8941 caccaaattt atgcagttga cgacctttcg cccctgcctg cgcaatcaca ctcattttta
     9001 ccccttgttt gcagcccgga agccattttc caggtcggca attaaatctt cgccatcttc
     9061 aataccggtg gagatacgca gcagcgtctc ggagatcccg gcggcagcac gcgcttctgg
     9121 tgccatgcct gcatgtgtca tggttgcggc gtgagagatt aaactttcca ctccccctaa
     9181 tgattccgcc agcgtaaaca acgacagccc gcccaggaaa cgacgcagcg tctgctcatc
     9241 gccatccagt tcaaaactca acattgcgcc aaagcctttt tgctggcgcg cggcaatttc
     9301 atgcccctga ttttccggca gcgacgggtg atacagtttc ttcaccaacg gctgggtttg
     9361 cagatatttc acaatcgcct gcgcgttgcg ctgcgccagc tccatacgcg gcaccagcgt
     9421 tcgcaacccg cgcagcagca gatagctgtc aaacgcgccg cccgtcacgc caatattgtt
     9481 tgcccaccag gcaagttcag tgacaacgtc cgggtcttta gcaatcacta cgcctgccac
     9541 cacgtcagag tgaccgttca gatatttcgt gcatgaatgc aacaccagat cggcacctaa
     9601 tgccagtgga ttttgtaatg ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc
     9661 gacttccctt gccagatggc aaattttcgc aatatccacg acgcgtaaca atggattact
     9721 tgggctttct accagtacca gtttgggttt ttctgccagc gctgcccgta atgcctgttc
     9781 atcgctttga tcaacaaaca acacgcgata gcaaccgcgt ttcgccaggc tgtcgaacag
     9841 gcgatagcta ccgccgtagc agtcgtgcgg cgcaaccagc agatcgccag gtttcaaaaa
     9901 gacggtcgtt accaggtgaa tcgcggacat gccggtatta gtaagtacgg caccggcacc
     9961 gccttccagt tctgccagcg cacgctgaac cacatcgcgc gttgggttgc cgcgacgcga
    10021 gtaatcatgc gcacgtggtt cattaaatcc ggtaaagtta taggtactgg aaagatggat
    10081 gggagggaca acgcaaccat actgttcgtc gtcatttaac ccgctacgca ctgcgatggt
    10141 ggcctgttta cgcgtcatgt gatgaagttc cctgggcttt gtcggtgaaa tgtcaggcac
    10201 cagagtaaac attgtgttaa tggacgtcaa tacatctgga catctaaact tctttgcgta
    10261 tagattgagc aaatcccaaa tagccgttaa aattatatgc attatcacgc cgacaggtgc
    10321 attacacgat gtcacggtaa cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc
    10381 acattaactg tgtggtctgg tctcaattta ttgacgaaga ggattaagta tctcatggct
    10441 gaatggagcg gcgaatatat cagcccatac gctgagcacg gcaagaagag tgaacaagtc
    10501 aaaaagatta cggtttccat tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg
    10561 cgtcgtcagg tgaacaacct gcgtcacgct accaacagcg agctgctgtg cgaagcgttt
    10621 ctgcatgcct ttaccgggca acctttgccg gatgatgccg atctgcgtaa agagcgcagc
    10681 gacgaaattc cggaggcggc aaaagagatc atgcgtgaga tggggattaa cccggagacg
    10741 tgggaatact aaagcggaaa agagcggcgc ggagttaacc agccgctttt ttcatacatg
    10801 gacgtttaac gatgaaaatc aggctactga tccccggcct gctggtttct gtgcctgcat
    10861 ttgcctggca accacaaacc ggcgacatca tctttcagat ctctcgctca tcgcaaagta
    10921 aagcgatcca actggcgacc cattccgatt acagccacac cggtatgctg gtgatacgca
    10981 acaaaaagcc ctacgtcttt gaagccgtcg gcccggtaaa atataccccg ctgaaacagt
    11041 ggatcgccca cggtgaagat agcaagtaca tcgtgcgtcg cgttaaaggc gagctaaatg
    11101 ccgaacaaca gcaaaaactg gcgcaaacgg caaaacgtta tctcggtaaa ccgtatgact
    11161 ccagcttctc gtggagcgac gatcgccagt actgttcgga agtggtgtgg aaggtctacc
    11221 agaatgcgct gggaatgcgt gtgggcgagc agcagaagct gaaagagttc gatcttagta
    11281 acccgctagt tcaggcaaag ctcaaagaac gctacggcaa aaatattccg cttgaggaaa
    11341 cggtagtttc accacaagcc gttttcgacg cgccacaact caccacggtc gccaaagaat
    11401 ggccgctgtt ttcctggtaa tacgaccaaa attcaggcac aaaaaaagcg ccgtgcggcg
    11461 cttttttcgg aaatccggtc ttatttgctg cccgggatgt tgaaacgctt gttgaagcgg
    11521 tcaacacggc caccggtagc aacatcacgc tgtttgccag tgaagaacgg gtggcacttg
    11581 ctgcacacgt cgaggttcag gtcatgacca acggtggagc ggattttcat tacgttaccg
    11641 caagagcagc tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt catgggaaaa
    11701 cctcggttta aggccgcgtc gctcttccag ccctaacgcc agacaccacg cgatgttaaa
    11761 agtatagctt caatacgatc atttcgtacg aaggcgcgaa atcatacaga aattaaccag
    11821 cgtatgcaaa ctgatccgca ctcttctacg gcaatgtgta tactaaccca ccgaatttca
    11881 agtcaggatg atgctatgcc cgttgcccac gttgccttgc ccgttccgct tcctcgtacc
    11941 tttgactatc tgctgccaga aggcatgacg gttaaagccg ggagtcgcgt gcgcgtgccg
    12001 tttggcaaac aacaggagcg catcggggtt gtggtgtcag tgagcgatgt cagtgagctg
    12061 ccgcttaacg agctgaaagc ggtagttgaa gtgctggacg ttgagccggt atttactcac
    12121 tccgtctggc gattactgct ctgggcggca gattactatc atcatccgat tggcgatgtg
    12181 ctgtttcatg ccttgccgat tttactgcgc caggggcggc ctgcggcaaa cgcgcctatg
    12241 tggtactggt ttgccactga acaaggtcag gcggtggatc tgaacagcct gaaacgttcg
    12301 ccaaagcaac aacaggcgct ggcagcgtta cggcaaggca aaatctggcg cgatcaggtt
    12361 gccacgctcg aatttaatga tgccgcgttg caggcactgc gcaaaaaagg tctgtgtgat
    12421 ttagcaagtg aaacaccaga gtttagcgac tggcgaacga actatgccgt ttctggtgag
    12481 cggttgcggt tgaataccga acaggccacc gccgttggcg caattcatag cgcggcagat
    12541 actttttctg cctggctgct ggcgggcgtt accggttccg gtaaaacgga ggtttatctc
    12601 agtgtactgg aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt gccggaaatc
    12661 ggcctgacac cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcccc ggtggaagtt
    12721 ctgcattccg gcctgaacga cagcgagcgt ctttcggcgt ggttgaaagc gaaaaatggc
    12781 gaggcggcga ttgtcatcgg cacccgctcc gcgctgttta cgccgtttaa aaatctcggc
    12841 gtgattgtta ttgatgaaga gcacgacagt tcctacaaac agcaggaagg ctggcgctat
    12901 catgcccgcg acctggcggt gtatcgcgcg catagcgagc aaatcccaat tattcttggc
    12961 tccgcaacgc ccgcactgga aacgttatgc aacgtgcagc agaaaaaata ccgcctgctg
    13021 cgcctgaccc gtcgggcggg caatgcgcgc ccggcaattc aacatgtgct ggatttaaaa
    13081 ggtcagaagg tgcaggcagg tctggctccg gcgttgatca cccgtatgcg ccagcattta
    13141 caggccgaca accaggttat cctctttctt aaccgccggg gttttgcgcc tgcgctactg
    13201 tgccacgact gtggctggat tgccgaatgc ccgcgttgcg atcactacta cacgctgcat
    13261 caggcacaac agcatttgcg ctgtcaccac tgtgacagcc aacgcccggt gccgcgccag
    13321 tgcccttcct gcggttccac acacctggta cccgtggggc tgggcaccga acagcttgaa
    13381 cagacgctcg cgccgctgtt ccccggcgtg cccatttctc gtatcgaccg cgataccacc
    13441 agccgcaaag gggcgctgga acagcaactg gcggaagtgc atcgcggcgg cgcgaggatc
    13501 ttgattggca cgcaaatgct ggcgaaaggt caccactttc cggatgtgac gctggtagcg
    13561 ttactggatg tggacggcgc gctgttttct gccgattttc gctcggcaga gcgtttcgct
    13621 cagctttaca cccaggtcgc tggtcgtgcc gggcgtgcgg gtaaacaggg cgaagtggtg
    13681 ctgcaaacgc accatccgga acatcctctg ttgcaaacgt tgctctataa aggctacgac
    13741 gactttgccg aacaggcgct ggctgagcga cggatgatgc agctgccgcc atggaccagc
    13801 catgtgattg tgcgtgcgga agatcataac aatcagcacg cgccgctgtt cctgcaacag
    13861 ttgcgtaatc tgatcctcgc cagcccgctt gcagacgaca aactgtgggt tctcggtccg
    13921 gttccggcac tggcccctaa gcgtggtggc cgctggcgct ggcaaatttt gttgcagcac
    13981 ccttcccgcg tgcgattaca gcacatcatc aatggtacgc tggcgcttat taatacaata
    14041 ccggattccc gtaaagtgaa atgggtgctg gatgtcgatc ccatcgaggg ctaaaccgct
    14101 cacgatgcga ggcggatcga aaaattcaat attcatcaca cttttcatga aaattctgta
    14161 accgttttca cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca tggatgtagt
    14221 acgcctgacg tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa actgccgcga
    14281 ccatgaaaga cgttgccctc aaggcaaaag tctctacagc gactgtctcc cgagcattaa
    14341 tgaatcccga taaagtctcc caggccaccc gtaatcgggt tgaaaaagcg gcccgggaag
    14401 tgggttattt accgcagcct atggggcgca acgtcaagcg taatgaatcc cgcaccattc
    14461 tggtgattgt cccggatatc tgcgaccctt tctttagcga aattattcgc ggtatcgaag
    14521 ttacggcggc aaatcacgga tatctggtgc tgattggcga ctgtgcgcat caaaatcagc
    14581 aggaaaaaac ctttatcgat ttgatcatca ccaagcaaat tgatggcatg ttgttgctgg
    14641 gttcaaggct gccgtttgat gccagcattg aggaacagcg taatctgccg ccgatggtga
    14701 tggcgaacga atttgcaccg gaactggagc tgcctaccgt tcatatcgac aatctgaccg
    14761 ccgcatttga tgcagtaaat tatttatatg agcaagggca taaacggatt ggctgtatag
    14821 ccggtcccga agagatgccg ctgtgtcact accgcctgca aggctatgtt caggcgctgc
    14881 gtcgctgcgg cattatggtt gatccgcaat acatcgcccg tggcgacttc accttcgaag
    14941 ccggaagcag agcaatgcaa cagctgcttg atcttccaca accgcctact gctgtcttct
    15001 gccatagcga tgtgatggcg ctcggcgcac tttctcaggc aaaacgtcag gggctgaaag
    15061 tcccggaaga cctttccata atcggttttg ataacatcga cctgacgcaa ttttgtgatc
    15121 cgccgctgac aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct atgctgttat
    15181 tgcttgatca aatgcagggg caacacgttg gcagtggctc tcgtttaatg gactgcgaac
    15241 ttatcatccg gggatcaaca cgcgcgttac cttaaagtaa acctttaaga ctgccgtgac
    15301 tggtcaaagg cccgccgctt aagtaacatg gcgggctgac gaacgaataa atacagcgaa
    15361 acgatagtgg cacaacgaga ttatgtacgc cgcagccaac cggcaccttc gcggcgaaaa
    15421 aagagcacct cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc tatggtcgct
    15481 attgctgccg ccgttcttgt gacctttatc ggtggtctgt acttcattac gcatcacaag
    15541 aaagaagagt ccgagacgct gcaaagccag aaagtgaccg gaaacggact accaccaaaa
    15601 ccagaagaac gctggcgcta cattaaagag ctggaaagtc gccagccggg tgtgcgtgcg
    15661 cccacagaac cttctgccgg tggtgaagtg aaaacgccgg agcaactgac accagaacaa
    15721 cgccagcttc ttgaacaaat gcaggccgat atgcgccagc agccaacgca gctggttgaa
    15781 gttccgtgga atgagcagac gccggaacag cgtcagcaaa cgctacagcg ccagcgtcag
    15841 gcgcagcaat tagcggagca gcaacgcctg gctcagcagt cgcgtacgac tgaacaaagc
    15901 tggcagcagc agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc gcgccagtct
    15961 aaaccggctt ctactcagca gccgtaccag gatctgctgc aaacccctgc gcacacgacc
    16021 gcgcagtcga agccgcagca agctgcgccg gttgctcgtg ccgctgacgc gccaaaaccg
    16081 acggcggaga aaaaagacga acgccgctgg atggtgcagt gcggttcgtt cagaggcgcg
    16141 gaacaggcag agacggtacg tgctcagctg gcgttcgaag gctttgactc aaaaattacc
    16201 accaacaatg gctggaatcg tgtggtgatt ggcccggtga aaggcaaaga gaacgcagac
    16261 agcaccctca atcgcttgaa gatggcgggt catacaaact gcattcggct cgccgccggg
    16321 ggttgaaacc ctcaaaatcc cccccatcta taattgcatt atgccccgta cttttgtacg
    16381 gggtttgtac tctgtattcg taaccaaggg gtcagctcgt gacaactata gtaagcgtac
    16441 gccgtaacgg ccatgtggtc atcgctggtg atggtcaggc cacgttgggc aataccgtaa
    16501 tgaaaggcaa cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc gcgggctttg
    16561 cgggcggtac tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa ctggaaatgc
    16621 atcagggcca tctggtcaaa gccgccgttg agctggcgaa agactggcgt accgatcgca
    16681 tgctgcgcaa acttgaagca ctgctggcag tcgcggatga aactgcatcg cttatcatca
    16741 ccggtaacgg tgacgtggtg cagccagaaa acgatcttat tgctatcggc tccggcggcc
    16801 cttacgccca ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc gcccgtgaaa
    16861 ttgctgaaaa ggcgttggat attgcaggcg acatttgcat ctataccaac catttccaca
    16921 ccatcgaaga attaagctac aaagcgtaag gatctcccat gtctgaaatg accccacgcg
    16981 aaatcgtcag cgaactggat aagcacatca tcggccagga caacgccaag cgttccgtgg
    17041 cgattgctct gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg cgccatgaag
    17101 tgaccccgaa aaatatcctg atgatcggcc cgaccggtgt cggtaaaact gaaatcgccc
    17161 gtcgtctggc taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc aaattcaccg
    17221 aagtgggcta tgtcggtaag gaagtggatt ctattattcg cgatctgacc gatgccgccg
    17281 tgaaaatggt acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa gaactggcag
    17341 aagaacgtat tctcgacgta ctgatcccac cggctaaaaa taactgggga cagaccgaac
    17401 agcagcagga gccgtccgct gctcgtcagg cattccgcaa aaaactgcgt gaaggccagc
    17461 ttgatgacaa agaaattgag atcgatcttg ccgcagcacc gatgggcgtt gaaattatgg
    17521 ctcctccggg catggaagag atgaccagcc agctgcagtc catgttccag aacctgggcg
    17581 gtcagaagca aaaggcgcgt aagctgaaaa tcaaagacgc catgaagctg ctgattgaag
    17641 aagaagcggc gaaactggtg aacccggaag agctgaagca agatgctatc gacgctgttg
    17701 agcagcacgg gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc ggcgagtctt
    17761 ccggtccgga tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg gtagaaggtt
    17821 gcactgtttc caccaaacac gggatggtca aaactgacca cattctgttt atcgcttctg
    17881 gcgcgttcca gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt cgtctgccaa
    17941 tccgcgttga gctgcaggca ctgaccacca gcgactttga gcgtattttg accgagccga
    18001 atgcctctat caccgtgcag tacaaagcac tgatggcaac tgagggcgta aatatcgagt
    18061 ttaccgactc cggtattaag cgcatcgcgg aagcggcatg gcaggtgaac gaatctaccg
    18121 aaaacatcgg tgctcgtcgt ttacataccg ttctggagcg tttaatggaa gagatttcct
    18181 acgacgccag cgatttaagc ggtcaaacta tcactattga cgcagattat gtgagcaaac
    18241 atctggatgc gttggtggca gatgaagatc tgagccgttt tatcctataa tcgcgttcaa
    18301 tcattttcat cattgtttga tggggctgaa aggccccatt tttattggcg cgtattatga
    18361 ctgaacaaca aattagccga actcaggcgt ggctggaaag tttacgacct aaaaccctcc
    18421 ccctcgcctt tgctgcaatt atcgtcggga cggcgctggc atggtggcaa ggtcacttcg
    18481 atccactggt cgccctgctg gcattgatta ccgccgggct attacagatc ctttctaacc
    18541 tcgccaatga ttacggcgat gcggtaaaag gcagcgataa acctgaccgc attgggccgc
    18601 tacgcggcat gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg ctcattatta
    18661 ccgtcgtgct catctgtctc tccgggctgg cactggttgc agtggcgtgc catacgctgg
    18721 ccgattttgt cggtttcctg attcttggcg ggttgtcgat cattgccgct atcacctaca
    18781 ccgtgggcaa tcgtccttat ggttatatcg gtttgggtga tatttccgta ctggttttct
    18841 ttggctggtt gagcgtcatg gggagctggt atttacaggc tcatacattg attccggcac
    18901 tgatccttcc ggcaaccgca tgcggcctgc tggcaacggc agtactgaac attaataacc
    18961 tgcgtgatat caatagcgac cgcgaaaacg gcaaaaacac gctggtggtg cgcttaggtg
    19021 cagtaaacgc gcgtcgttat catgcctgcc tgctgatggg ctcactggtg tgtctagcgc
    19081 tgtttaatct cttttcgctg catagcctgt ggggctggct gttcctgctg gcggcaccgt
    19141 tgctggtgaa gcaagctcgt tatgtgatgc gcgaaatgga cccggtggcg atgcgaccaa
    19201 tgctggaacg cactgtcaaa ggagcgttac tgactaacct gctgtttgtt ttagggatat
    19261 tcctaagcca gtgggcagca taactgacaa atatcaatta acaattgatg attttgccaa
    19321 cagcccacat agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta agcctatgaa
    19381 atacgatact tccgagcttt gtgacatcta tcaagaagat gttaacgtcg tggaaccgct
    19441 gttctccaac tttggcggac gggcgtcgtt tggcggacaa ataatcacgg taaaatgttt
    19501 cgaggacaac gggttgctgt acgatctgct cgaacagaat ggccgtggtc gtgttcttgt
    19561 cgtcgatggc ggaggttctg ttcgccgcgc actggtcgat gctgaactgg cgcgtctggc
    19621 agtacaaaat gaatgggaag gcctggtcat ttacggcgcg gtgcgtcagg tagatgacct
    19681 ggaagagttg gatatcggca tccaggcgat ggcggcaatt ccggttggtg ccgctggcga
    19741 aggcattggc gaaagcgatg tccgcgtcaa ttttggcggt gtcaccttct tctccggcga
    19801 ccatctttat gccgacaata ccgggattat tctttcagaa g
//
LOCUS       DAOFSV010000080        19453 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.080, whole genome shotgun sequence.
ACCESSION   DAOFSV010000080 DAOFSV010000000
VERSION     DAOFSV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.080"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(27..1022)
                     /gene="gapA"
                     /locus_tag="QBH27_002941"
     CDS             complement(27..1022)
                     /gene="gapA"
                     /locus_tag="QBH27_002941"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDQ7015228.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1364..1777
                     /gene="msrB"
                     /locus_tag="QBH27_002942"
     CDS             1364..1777
                     /gene="msrB"
                     /locus_tag="QBH27_002942"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDQ7015229.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1819..2091
                     /locus_tag="QBH27_002943"
     CDS             1819..2091
                     /locus_tag="QBH27_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDQ7015230.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            2461..3537
                     /locus_tag="QBH27_002944"
     CDS             2461..3537
                     /locus_tag="QBH27_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ7015231.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVTVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            3564..4943
                     /locus_tag="QBH27_002945"
     CDS             3564..4943
                     /locus_tag="QBH27_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015232.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            4960..6003
                     /locus_tag="QBH27_002946"
     CDS             4960..6003
                     /locus_tag="QBH27_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDQ7015233.1"
                     /translation="MKNSKAMLQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            6024..6860
                     /locus_tag="QBH27_002947"
     CDS             6024..6860
                     /locus_tag="QBH27_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDQ7015234.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAIKERALDKIKLFGSDGK
                     AE"
     gene            6865..7812
                     /locus_tag="QBH27_002948"
     CDS             6865..7812
                     /locus_tag="QBH27_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDQ7015235.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            7822..8802
                     /gene="ydjG"
                     /locus_tag="QBH27_002949"
     CDS             7822..8802
                     /gene="ydjG"
                     /locus_tag="QBH27_002949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDQ7015236.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            8939..9697
                     /locus_tag="QBH27_002950"
     CDS             8939..9697
                     /locus_tag="QBH27_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7015237.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFVDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            9815..11173
                     /locus_tag="QBH27_002951"
     CDS             9815..11173
                     /locus_tag="QBH27_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015238.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWMPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(11266..11907)
                     /gene="pncA"
                     /locus_tag="QBH27_002952"
     CDS             complement(11266..11907)
                     /gene="pncA"
                     /locus_tag="QBH27_002952"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDQ7015239.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(11918..12934)
                     /gene="ansA"
                     /locus_tag="QBH27_002953"
     CDS             complement(11918..12934)
                     /gene="ansA"
                     /locus_tag="QBH27_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDQ7015240.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQKLDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(13066..13776)
                     /locus_tag="QBH27_002954"
     CDS             complement(13066..13776)
                     /locus_tag="QBH27_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDQ7015241.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNIPGELFPKLEDAQKKIVAELNAQGINEKEITLGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATEGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            complement(13892..15748)
                     /gene="sppA"
                     /locus_tag="QBH27_002955"
     CDS             complement(13892..15748)
                     /gene="sppA"
                     /locus_tag="QBH27_002955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDQ7015242.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEVQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            15909..16460
                     /locus_tag="QBH27_002956"
     CDS             15909..16460
                     /locus_tag="QBH27_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDQ7015243.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            16577..17620
                     /gene="selD"
                     /locus_tag="QBH27_002957"
     CDS             16577..17620
                     /gene="selD"
                     /locus_tag="QBH27_002957"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDQ7015244.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGVSFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            17625..>19453
                     /gene="topB"
                     /locus_tag="QBH27_002958"
     CDS             17625..>19453
                     /gene="topB"
                     /locus_tag="QBH27_002958"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDQ7015245.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETYKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTS"
BASE COUNT         4919 a         4475 c         5050 g         5009 t
ORIGIN      
        1 tttttagatc acagtgtcat ctcaacttat ttggagatgt gagcgatcag gtccagaact
       61 ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa gttgtcgttc
      121 agagcgatac ccgctttagc atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta
      181 gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc agcagcagct
      241 ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca
      301 actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt gccattcagt
      361 tctggcagta ctttacctac agctttagca gcaccggtag aagacgggat gatgttctgg
      421 gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt tttctgagta
      481 gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt atcgttgata
      541 actttagcca gcggagccag gcagttggtg gtgcaggatg cgttggaaac gatgtcctgg
      601 ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc tttagacgga
      661 ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc
      721 aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca tttcaggtta
      781 gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag atgaccgtct
      841 ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag catgtatgcc
      901 atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga acgtttctga
      961 gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac tttgatagtc
     1021 atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct
     1081 tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca
     1141 ttacgcgact gactcgcctc actcttcctt tgggcttgag accacatggg gtcggcgccc
     1201 cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta ggtgcagcca
     1261 tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa
     1321 tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta ataaaccttc
     1381 ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttc tacgtgacgc agaatcatgg
     1441 gacagaaccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg tatatcactg
     1501 tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg gctgtggctg
     1561 gcccagtttc tacgaaccgg tgagtgaaga atctattcgt tatatcaaag acttgtcaca
     1621 tggaatgcag cgcatagaaa ttcgttgcgg taactgcgat gcccatctgg gccatgtctt
     1681 tcctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt ctttacgctt
     1741 cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag caacttattc
     1801 cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac gcctgaggta
     1861 taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt tacgttgacc
     1921 gaggaacaaa aagagaactg cctgcaactg gtgatgctgt ggcaagcccg ccataatacc
     1981 gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag caaacagcag
     2041 ttaaaagaag attttggtat ctcagcaaag cctattgcca tgtttaagta agtggtttat
     2101 cccctttttg ccgcggttat aaaggggata gccaacaatg accccacgac tcatctcaat
     2161 tatctcgccc cgacctggct aagtcccagt ccgatctctt gcagtcgcgt tcacacctga
     2221 atgacttctc ctgaacttaa gtctcaggcc tatttggccg ggaatccctc tcgaatttgc
     2281 tctttttgcg tcccgccttc catcaacctg taacaacccc agccattttc tgcgtaattg
     2341 tgatctaagt tgtaaaacgc agatcattat ctgtgtttta cagatttaat gttgacctca
     2401 aaaatcatgt gggtaagtat gatgaccatc taagcaaaac aaaacacaga ggatgacgca
     2461 atgaaagcac tggctcggtt tggcaaggct tttggtggct acaagatgat tgatgtccca
     2521 caacccattt gtggcccgga agatgtcgtg attgaaatta aagccgcggc aatctgcggc
     2581 gcagacatga agcactacaa tgtcgatagc ggttcggatg agtttaactc tatccgcggc
     2641 catgagttcg caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg gaaagtgggg
     2701 caacgcgtcg tgtcggataa cagcggtcac gtttgcggcg tttgcccggc ctgtgaacaa
     2761 ggtgattttc tgtgttgtac agaaaaagta aaccttggtc tggataacaa tacctggggc
     2821 ggtggttttt ccaaatattg tcttgttcct ggtgaaattc tcaaaattca tcgtcatgcg
     2881 ttgtgggaaa tccctgatgg tgttgattat gaggacgcag ccgtacttga ccctatctgt
     2941 aatgcctata aatccatcgc gcagcaatcg aaattccttc caggtcagga tgtggtcgtc
     3001 atcggcactg gcccactcgg gctgttctcc gtacaaatgg cgcggattat gggggcggta
     3061 aatatcgtcg tcgttggtct gcaagaagat gtaacggtcc gcttcccggt tgcaaaagaa
     3121 ctgggtgcga cggcagtagt aaatggttct accgaagatg tggtggcacg ctgccagcaa
     3181 atttgtggca aagacaatct ggggctggtg attgaatgct ccggtgccaa tatcgcattg
     3241 aaacaagcca tcgaaatgct ccgcccgaat ggggaagtag tacgcgttgg aatgggcttc
     3301 aaacctcttg atttctcgat taatgacatt accgcctgga acaaaagcat cattgggcat
     3361 atggcctatg actccacttc atggcgtaac gctatcaggc tattagccag cggcgctatc
     3421 aaagtcaaac cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga agggtttgat
     3481 gcgatggtcg ataaaactgc aatcaaagtg atcatgactt acgactttga tgaataacta
     3541 ctctcctgaa ggaacgttat cgcatggaac agataacaaa accgcattgt ggtgcacggc
     3601 tggatcgctt accggattgc cgctggcatt cgtcgatgtt tgccatcgtc gcgtttggcc
     3661 tgcttgtctg ctggagtaat gccgttggtg gcttgatcct cgcgcagctg aaagcgttgg
     3721 gctggacaga taattccacc actgccacat tctcagcaat cacgaccgcc ggaatgtttc
     3781 tcggtgcgct ggtgggcggc atcatcggtg acaaaaccgg tcgcagaaat gcgtttatcc
     3841 tctatgaggc cattcatatt gcctcgatgg ttgtcggtgc tttctcaccg aatatggatt
     3901 tccttattgc ctgccgtttt gtgatgggcg ttgggctggg agctttactg gttacactgt
     3961 ttgctggttt caccgaatat atgcccggaa gaaatcgcgg aacgtggtcc agtcgggttt
     4021 cctttattgg taactggtca tatccgctct gttcattgat tgcgatggga ctcacgccgc
     4081 tgattagtgc agagtggaac tggcgggtac aactgcttat ccctgcaata ttgtcactta
     4141 tcgctacggc gctggcctgg cgctactttc ctgaatcccc gcgctggctg gaatcgcgcg
     4201 gacgttatca ggaagccgag aaagtcatgc ggagtataga agaaggcgtc atacgccaga
     4261 cgggtaaacc tttgccgcct gtggttattg ctgatgacgg taaagcacca caagcggtgc
     4321 cgtattcagc cttactgaca ggagtattac tgaaacgcgt gatattaggc tcttgtgtgc
     4381 tgattgccat gaacgttgtg cagtacacac taattaactg gttgccaacg atattcatga
     4441 cccagggaat taatttaaaa gactcgattg ttttaaatac catgagtatg tttggcgcgc
     4501 catttggtat ttttattgcc atgctggtga tggataaaat tccgcgtaaa acaatgggtg
     4561 tggggctatt aatcctgatt gcggtgctcg gatatatcta ttcactgcaa accagtatgt
     4621 tgctcataac gctgattggt ttcttcctga ttactttcgt ctatatgtac gtttgctatg
     4681 cctcggcagt gtatgtccct gagatctggc cgacagaggc caaactccgt ggctccggtc
     4741 tggcgaatgc ggtagggcga atcagtggta ttgccgcacc ttatgccgtt gcagtgctgc
     4801 tcagtagtta tggcgtaacg ggagtcttta ttcttctggg ggctgtttca attattgtcg
     4861 caattgctat cgccaccatt ggaattgaaa ccaaaggtgt ctccgttgaa agtttaagta
     4921 ttgatgcagt agccaataaa taatactggt ggattcaaaa tgaaaaattc aaaagcaatg
     4981 ttgcaggtgc cgggcacaat gaaaattatt tcagcagaaa tacctgtacc taaagaagat
     5041 gaagttttga ttaaagtgga atatgtcggt atttgtggtt cagatgtaca tggttttgaa
     5101 tcaggcccgt ttattcctcc taaagaccca aatcaagaaa ttggcctggg tcatgaatgc
     5161 gccgggacgg ttgtggctgt gggaagccgc gtgcgcaaat ttaaaccggg ggatcgggta
     5221 aatatcgaac ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg caaatataac
     5281 atctgcccgg acgttgattt tatggcgaca caacccaact accgcggcgc attaacgcac
     5341 tatctgtgtc atccggagag ctttacttac aaactgccag acaatatgga cacgatggaa
     5401 ggggcgctgg tggagcctgc cgcagtcggg atgcatgccg cgatgctggc agatgttaaa
     5461 ccgggtaaga agataattat tctgggagcg ggttgtattg gtttgatgac gttgcaagcg
     5521 tgcaaatgcc tgggagcaac ggaaattgcc gtcgttgatg tgctggaaaa acgtctgaca
     5581 atggcggaac agctcggcgc gacagtggtt attaacggcg caaaagaaga cactattgca
     5641 cgctgtcagc aatttaccga agacatgggc gcagatattg tttttgaaac agcgggttct
     5701 gcggtcaccg ttaaacaggc accttatctg gtcatgcgcg gcggtaaaat tatgattgtt
     5761 ggtactgtac ccggcgattc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc
     5821 cagacggtat tccgctatgc caatcgttat ccggtcacga ttgaagctat ttcttcaggg
     5881 cgattcgatg tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca
     5941 tttgaagagt cagttaacaa caaacgcgac attattaaag gcgttattaa aattagcgat
     6001 taattaatac gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca
     6061 caaataaaca ttatgcaatt gcccatttta acgtatggaa tgcagaaatg ttgatgggcg
     6121 ttatcgacgc agccgaagaa gcgaaatcac cggttattat ttcttttggc acaggttttg
     6181 tcggcaacac ctcatttgaa gatttctctc acatgatggt atcaatggca caaaaagcaa
     6241 cggtgccggt aataactcat tgggatcatg gtcggagtat ggagattatt cataacgcct
     6301 ggactcatgg tatgaattca ttaatgcgtg atgcttctgc atttgatttc gaagaaaata
     6361 ttcgtttaac caaagaggct gttgatttct tccatccgct gggtattccg gtagaggcgg
     6421 aattagggca tgtcggtaat gaaaccgttt atgaggaagc gctggcaggc tatcactata
     6481 ccgatcctga ccaggcggct gaatttgttg aacgtacggg ctgtgactca ttggctgtcg
     6541 ccatcggcaa ccagcatggg gtttatacgt cagagccaca attgaacttt gaggtcgtca
     6601 aacgcgtacg cgatgctgtt tctgttccgc tggttttgca tggcgcatca gggatcagtg
     6661 atgccgacat taaaactgca atttcgttgg gtattgcgaa aatcaacatt catacggagc
     6721 tctgtcaggc cgcaatggta gcagtgaaag aaaaccagga tcagcctttc ctgcatttag
     6781 aacgtgaagt gcgtaaagct ataaaagaac gggcattgga taaaattaaa ctgttcggtt
     6841 ctgatggcaa agcggaataa taatatggat aatctcgacg ttatttgtat aggtgccgct
     6901 attgttgata ttccattgca accggtcagt aaaaatatct ttgatgtgga ttcttatcct
     6961 cttgaaagaa tcgcaatgac caccgggggt gatgcaataa atgaagcaac aattatttct
     7021 cgtctgggcc atcgcacagc attaatgagt cgtattggta aagatgccgc agggcaattt
     7081 attctcgacc attgccgcaa agagaatatt gatattcaaa gcctgaagca ggacgtcagc
     7141 atagatacct ctattaacgt tggactggtg acggaggatg gcgagcggac ctttgtcaca
     7201 aaccgtaacg gcagtctgtg gaaattgaat attgacgatg ttgattttgc gcggttttct
     7261 caggcgaaat tattatcact ggccagtatt ttcaacagtc ctctattgga tggtcgagcg
     7321 ctaacagaaa tttttacgca agctaaaacc cggcagatga ttatctgtgc cgatatgatc
     7381 aaaccgcggt tgaatgaaac gctgaatgat atttgcgaag cattgagtta tgtcgattat
     7441 ctgtttccta attttgccga ggcaaaatta ctcaccggga aagagacact ggatgaaatt
     7501 gctgactgct ttcttgcgtg cggcgtaaaa acggtggtga ttaaaacggg taaagacggc
     7561 tgctttatca agcgtggtga catgacgatg aaggtgccgg cggtcgcagg aataaccgct
     7621 atcgacacca ttggtgcggg cgataacttt gcttcaggtt ttattgcagc actgttagaa
     7681 ggcaaaaatc tgcgagaatg cgcacgcttt gccaatgcaa cggcggctat ctcggttcta
     7741 agcatcggcg ccaccaccgg tgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa
     7801 tacgaaggat aatgaaggca aatgaaaaag atacctttag gcacagcgga tataacgctt
     7861 tcgcgaatgg ggttgggtac atgggctatt ggcggcggtc ctgcatggaa tggcgatctc
     7921 gatcgacaaa tatgcattga tacgattctt gaagcccatc gttgcggcat taacctgatt
     7981 gatactgcgc caggatataa ctttggcaat agtgaagtta tcgttggtca ggcgttaaaa
     8041 aaactgcccc gtgaacaggt tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa
     8101 ggaagtttat tcaacaaagt tggcgatcgg cagttgtata aaaacctttc tccggaatct
     8161 atccgcgaag aggtagaagc cagcttgcaa cgtctgggta ttgattacat cgatatctac
     8221 atgacgcact ggcagtcggt gccgccattt tttacgccga tagctgaaac tgttgcagtg
     8281 cttaatgcgt taaaggccga agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct
     8341 gaccatatcc gcgagtatct gcaacatggt gaactggata ttattcaggc gaaatacagt
     8401 attctcgacc gggcgatgga aaacgaactg ctgccgctat gtcgtgataa tggcattgtg
     8461 gttcaggttt attccccgct agagcaggga ttgttgaccg gcaccatcac gcgtgattac
     8521 gttccgggcg gcgctcgggc aaataaagtc tggttccagc gtgaaaacat gctgaaagtg
     8581 attgatatgc ttgaacagtg gcagccactc tgtgctcgtt atcagtgcac aattcccact
     8641 ctggcactgg cgtggatatt aaaacagagt gatttaatct ccattcttag tggggctact
     8701 gcgccggaac aggtacgtga aaatgtcgcg gcactgaata tcaacttatc ggatgcagac
     8761 gcaacattga tgagggaaat ggcagaggcc ctggagcgtt aaatactcaa cacgattcac
     8821 ctctgtttct gtgaaaactg tgatgaaaat ctgtgattca aacaggttat tttgaaagta
     8881 aacatcggtt atgcgataat tgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt
     8941 ggcggcaaaa gacaggattc aggcaattaa gcaaatggtt gccaacgata aaaaggtgac
     9001 agtctcaaat ttgagtggga tttttcaggt aaccgaagaa accattcgcc gcgatcttga
     9061 gaagttggaa gatgaaggct ttttgaccag aacctatggt ggtgctgttt taaatacagc
     9121 gatgttgacg gagaatatcc atttttataa gcgcgcttca tcgttttatg aagagaagca
     9181 gcttattgca cgcaaggcac taccctttgt cgacaataaa accaccatgg cagccgattc
     9241 cagtagtacc gtaatggaat tgctcaaatt gttacaggac cgtagtgacc tgacgttgct
     9301 aaccaactcg gcggaagcga ttcatgttct ggctcagtca gaaattaaag tcgtttcaac
     9361 gggtggggaa ctaaacaaaa atacactttc actgcaagga agaattacta aagagatcat
     9421 cagccgctac catgtcgata tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg
     9481 cgcgctggac tctaatgaag cggaagctga aatcaaaaag acaatgatcc gtcaggcgac
     9541 agaagttgcg ttattggttg atcactctaa gtttgatcgc aaagcttttg tccagttagc
     9601 tgactttagt catattaatt acataataac tgataaatca ccgggtgcag agtggatagc
     9661 attttgcaaa gacaataata tccagctggt gtggtaattt aagcacacat tgtcaatatt
     9721 atttgttcct gcgcctggaa ataaaatgat gataaagatg cggcaagtga atttgccgca
     9781 attgctacct tttttagcaa atccgaggca cccaatggaa caatatgatc aaattggcgc
     9841 aagactggac cgcttgcctt tggcccggtt tcattatcgt atatttggta taataagctt
     9901 tagtctgtta ttaacggggt ttttgagtta ctccggtaat gtcgtcttag caaagctggt
     9961 aagcaatgga tggtcaaata atttcctcaa tgccgccttt acctcggcat taatgtttgg
    10021 ttatttcatc ggctctctta ccggtgggtt tattggtgac tactttgggc ggcgcagggc
    10081 gtttcgcata aatcttctca tcgtcggtat tgcggcaaca ggggccgctt ttgtccctga
    10141 tatgtactgg ctcatcttct ttcgcttcct gatgggaaca ggaatggggg cgctgattat
    10201 ggttggctat gcctcattta cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc
    10261 gcggctctca tttgttggaa actggtcgcc catgctgtct gcggcgatag gcgtggtggt
    10321 tatcgctttt tttagttggc ggataatgtt tctgctgggc ggtattggca tactgttagc
    10381 ctggtttctc tcaggtaaat actttattga gtcgccacga tggttggcag ggaaagggcg
    10441 aatcgcaggt gcagaaagcc aacttcgtga agtagagcag caaattgaaa gagagaagag
    10501 tattcgttta cccccgctta ctttgaacca gagcaacagc aaggttaagg taatcaaggg
    10561 taccttctgg ctcctgttta aaggggaaat gttacgacgt acattagtcg cgattactgt
    10621 tttaattgca atgaacattt cgctttatac catcaccgta tggatgccga ccatatttgt
    10681 taactccggc attgatgtcg ataaatcaat attaatgacc gctgttatta tgattggcgc
    10741 tccggtaggg atatttattg cggcattaat tattgatcat ttccctcgtc gattatttgg
    10801 ttccgcctta cttattatta ttgccgtgtt aggctatatc tattcaattc agacaacaga
    10861 gtgggcgatt ttaatctatg gcctggtgat gatcttcttt ttatacatgt atgtttgctt
    10921 cgcgtcggcg gtttatatcc cggaactttg gccaacgcat ttacgcctgc gtggttcggg
    10981 tttcgttaat gccgtaggac ggatcgtcgc agtcttcacg ccctatggcg ttgcggcatt
    11041 attaacacat tatgggtcga tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg
    11101 tgcgctggtt ctctccattt ttggcatcga aacgcggaag gtgtcgctgg aagagatttc
    11161 tgaggtgaat taaatctttc gttgatggcg tcaattacca gatgtatcac tgatgtgttt
    11221 tatctggcct acaatgccag tacagaattg taggccagcg taaaattact cctgcgtttc
    11281 ttcccagtct gccagcgtat atagcgttgc cccagctgct gacatttcca taaacgcgtg
    11341 cgcactgtcc tgtggctgga tatttacgcc acgacaacca tcggtaatca cgttcacctt
    11401 atagcctaac tgtaaagcgt ccagcacggt aaacttaacg caatagtcag tagccagacc
    11461 cataacgatc aattcattga ttacatgagc gcgcaaccag tcatcgagcg cagttttctg
    11521 ccgacgaccg ttatcaaaaa aggcactgta actgtcaact aaaggatttt cgcctttatg
    11581 gaacaccgct gcgatcgctt tttgcttcag taacggatgt aattgtgcgc cttcactgtt
    11641 ctgcacacag tgatcgggcc agaaggtttg tggcaaaccg tcgagttggc ctggcgtata
    11701 aggctctaca ccgtgctgac tggcaaaact gccgtgattc gccggatgcc agtcctgact
    11761 ggcgataact gcttcaccgc gcgactggca ccagtcaatc aggcggttag cgacatccac
    11821 cgtactgtca ccttccggca cggcgagcgc gccaccagca cagaaatcat tttgtaaatc
    11881 gaccagtaac agggcgcgag ggggcattgc agtctcctta atcatccggc gtcagttcgc
    11941 cacgcaggtt ttggctcatg gccttgcgaa tggtttcagt atcaagtttc tggctaagca
    12001 ggtaatgcag tttggttagc gtggcttcta cagtcatatc tgcaccgcca ataacgccgg
    12061 catgggcgag ggcgttaccg gtggcataac cacccatgtt cactttaccg gacatacatt
    12121 gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg
    12181 ctttgttttg tggcgcatta cccacgccat atgagcgcag aatcaatgct ttcacaggtt
    12241 ggcgcagaaa attgcgcacg acgtcagcag aaatccccgg ataaatcgtc actacgccaa
    12301 ttggttgcgg cgtgattgga tgaacgatca attccccttc gccgtgcggg gcgggtggcg
    12361 tattcaaacg acgaatatgg atacctgctt ccagtaacgg aggaaggttt ggagaggcaa
    12421 acgcatcaaa accatcggca tgggctttgg tggttcggtt gccacgatac agtcgattat
    12481 taaaaaagag agttacttcg ttgataggat aattcgccgc aacgtacaac gcattcagca
    12541 gattaatttg tccgtcagag cgtaactcag ccagcgggat ttgtgaccct gtcacaatga
    12601 ccggtttact gagattctcg agcatgaacg acagtgcaga ggcggtatac gccatcgtgt
    12661 ctgtgccgtg cagaatgaca aaaccatcat agtcgtcata gtgcgcttta atatcttcag
    12721 caatatgctg ccagtcttct ggcgtcatat cagaagaatc catcaatggc gtatattcat
    12781 gaatggtgaa atctggcatc tccgggcgat ggaattccgg catcagcgcc agttggcgct
    12841 gtagatgacc tgacaccgga atataaccct gctcggaacg ctgcatcccg atggtcccgc
    12901 ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt gatatgaatg atatcgaaag
    12961 agcaaaagta tatacgtgag gggcagcgag cgcacctgga tactctgtgg atatgagaaa
    13021 agtccggcag aacggaggga ttagtgtccc gccggagaaa agtggttaat cctgcaacgc
    13081 ataggttacg gtggtgacta cgcgtaccgt tttattaatc gccgtaggat catcttcatc
    13141 actgccactg gcgaagatct ggaacacgcc ttgtgaggca tcgctaattg accccacctg
    13201 gctacctgag tccgttgcaa attgtaatgc tgcgttgcgt gcatctttgg tggcggcggc
    13261 aatcatctca gcgcgcagtg cgccaatacc attaaagcga taggcgactt tgctctcagc
    13321 gatagcgccc tccgtagcga cttgcagttc gtttaatttc gcgacgactt tttcgacagc
    13381 agcaacatta tgggttttta tcgtcactga cccgtctgcg ttatagcgtg gcattgtggg
    13441 atcatctttc agataaaagg aatctgttcg cttgcttgtc cattggccca gggttatctc
    13501 tttctcgtta atcccctgag cattgagttc ggcaacaatt tttttctgcg cgtcttccag
    13561 tttcgggaac agttcgccgg gaatattacc cttaaagtta atcgcaattg ttaattctgc
    13621 cgtatctgcg cgaacttctt tttcagaaag cccacgcaca tttacataac ggttattggt
    13681 ttttaaatgt ttaacaccat cgccaacaaa ataaccacag gcgctaatcc ccagtccgag
    13741 gacgatagcg gcaattaaag agggggcctt attcactgat gactccatat cagatgctaa
    13801 atgagcaagg gaatgtagtg gagagaggga gtgaaagaca gtccgattca ggagtgtggc
    13861 cgacagcata tcggccacat ttttgcagga cttaacgcac gttggcgcag gtcaggcaaa
    13921 acgcataacg gttttgtggg tcgttaaacg cggccagctt gtcgctttca cttttaacgg
    13981 tagaggctac cgaggccagc ggtgcaggta acatggcctg gaacgcatct ggcaacattg
    14041 cccggacaga accagacatg ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt
    14101 agtattccag atgccactgt ttcacttttg ccagctctgc tgctttggcg accgcatcat
    14161 cgaaatcccc gagactatcg accagcccgt tagcttttgc atcctgaccg gtccagacgt
    14221 ggccctgggc gattttatca atctgctccg gcgttgaatg acgcgcatca gcaaccagcg
    14281 tgataaagcg tttatagcca ttctcaatgc ttaactgcat catctgctgc acttccggcg
    14341 gcagtgccct ggtgatagaa acatccgcca gcggtgaagt tgagacacca tcagtatgaa
    14401 caccaatcga atccagacta ttttctacgg tggtgatcac gccgaagata ccgatagaac
    14461 cggtcagggt gctggggtta gccacaatgt aattagctgg tgtggaaatc cagtaaccac
    14521 cagatgccgc catgccaccc atcgatacaa ccacaggctt acccgctgcc cgggctgctg
    14581 ccagttcagc gcgaatcact tcagacgcgg taacgctgcc gccgggacta ttaacacgca
    14641 gaacaatcgc tttcactttc gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat
    14701 caccgccaac attcccctga gtttcctcgc catccataat tgcgccatta gcgaagacga
    14761 caccgatact gtcaccggta tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg
    14821 cgcgataatt tttatcagtt ttactccagc cgaactcttt ggtcagagtt ttttcgattt
    14881 cggcactcga tgccagtgca tcgaccagct tgttttccag cgcatatttc gcggtatcgc
    14941 caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag
    15001 ggatctggcg gttagcggca acagtattca gatagttttg ccacagctca ccaatccagc
    15061 ggctgtcagc ttcgcgggct gccggcgaca tatcatcacg aataaacggt tcaacggcag
    15121 atttatacgt acccacgcgg aacacatggg tggaaacttt cagcttatcc agtaacgatt
    15181 tgtagtacag accgttggtg gcaaagccgt gcagatcaac cacgccttgc ggagacagcc
    15241 aaattttatt ggcgaaactg gcgagataat attgcccctg gctgtagttc tcgccaacgg
    15301 cataaaccgg tttcccgctg tcacgaaact ctttcagagc tttgccgatg tactgcatag
    15361 acggctggtc gccgcctgcg aagtttttca gatccatcac aataccggtg atattgcggt
    15421 cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag tgagttttcc tgaagacgat
    15481 cggaactggc accaagcagc tggcggctta atttactaaa ccgctgagaa ctgtcgggtt
    15541 tatcgacgat cacaccagaa atgtccagca gcagtgcgcc acgactggcc gtttctttcg
    15601 aatcaccacc actgacctgc atccagatcc ccacaccaac cagcacgaga aaaataaaga
    15661 acaggttaag taccatttca cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc
    15721 cggcaataaa tcgccaaagg gttcgcatgt attctcccaa cttaaggtca cacctgtttc
    15781 aggcgcaata gatgtggtta tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg
    15841 actgggctgt aacaaaatcc actctcgtgt taattttgtg aataaatatc atgacaggag
    15901 ttaatcaaat ggatgcactc gaactattga tcaatcgccg tagcgcctcc cgcctggctg
    15961 aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg
    16021 accataagtc catgcaaccg tggcatttct ttgtgattga aggggaaggg cgcgagcgtt
    16081 tcagcgccgt actggagcag ggggcgattg ctgccggtag tgataacaaa gcaatcgaca
    16141 aagcgcgtaa tgcgccgttc cgcgcaccgc tcatcataac ggtggtggcg aaatgtgaag
    16201 aaaatcataa agtcccgcgc tgggaacagg aaatgtctgc gggatgcgcg gtcatggcga
    16261 tgcaaatggc agcagttgcc caggggtttg gcggcatctg gcgcagtggt gcattaactg
    16321 aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca ggataaaatt gtcggttttc
    16381 tctacctcgg tacgccgcag ttgaaagcat ctacgtcgat aaacgtcccg gacccgacgc
    16441 cgtttgtgac ttatttctga taattgtcgc gaaactgtct ggattgtgag caaaggcgca
    16501 ggaattcaga cactctcact tatcacttca tggaatgagg gttaccatac ccggattgca
    16561 ttgacaggag atgtccatga gcgagaactc gattcgtttg acccaataca gccacggcgc
    16621 tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc
    16681 gaagtttgtt gatccgaatt tgcttgtggg taatgaaacc cgcgacgatg cggcggtgta
    16741 cgatctgggc aatggcacca gcgttatcag taccaccgac ttctttatgc cgatcgttga
    16801 taatcctttc gattttggcc gcattgcggc gactaacgcc atcagcgata tctttgctat
    16861 gggcggcaaa ccgattatgg cgattgcgat cctcggctgg ccgattaaca aactttcccc
    16921 ggaaattgcc cgcgaagtga ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc
    16981 gctggctggc ggtcactcca tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg
    17041 gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca caagccggat gcaaactgtt
    17101 cctgacgaaa ccgctgggga ttggcgttct taccacggct gagaaaaaat cactgctgaa
    17161 accagaacat cagggactgg cgacggaagt gatgtgccgg atgaacatcg caggcgtgtc
    17221 ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt acgggttttg gtttactggg
    17281 gcatttaagc gaaatgtgtc agggcgcagg tgtgcaggcg cgcgtcgact atgacgcgat
    17341 cccgaaattg ccgggggttg aagagtacat taagttgggc gcagtgcctg gcggcaccga
    17401 acgtaacttt gccagctacg gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgatct
    17461 gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa
    17521 tgaggtcaaa gctacagccg ccgagtttgg cattgaactg acggcgattg gcgaactggt
    17581 gccagcgcgc ggcggtcgtg ccatggttga gatccgttaa ttcaatgcgg ttgtttattg
    17641 ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct gcctaaaccg caccggaaag
    17701 gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac ctggtgtatc ggtcacctgc
    17761 ttgagcaggc gcagccagac gcctacgaca gccgctatgc gcgctggaat cttgcggatt
    17821 tgccaattgt cccggaaaag tggcaattac agccccgacc ctccgtgact aaacaactta
    17881 atgtcatcaa acgcttcctg catgaagcca gcgaaatcgt tcacgccgga gacccggatc
    17941 gcgaagggca actgctggtg gatgaagtgc tggactacct gcaactggca ccggaaaagc
    18001 gccagcaggt acagcgttgt ttgataaacg acttgaaccc gcaggcggtt gagcgggcga
    18061 tcgatcgtct tcgctccaat agcgagtttg taccgctgtg cgtttctgca ctggcgcggg
    18121 cgcgggctga ctggctgtac ggcattaata tgacccgtgc gtataccatt ctcggccgca
    18181 atgccggtta tcagggtgtg ctttccgtgg gacgcgtgca gacgcccgta ctcggactgg
    18241 tggtgcgccg cgacgaagag attgaaaact tcgtggcgaa agacttcttt gaagtcaaag
    18301 cacatatcgt gacacctgcc gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt
    18361 gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg tccactggcg gagcatgtgg
    18421 ttaaccgtat tagtggtcaa ccagctattg tcaccagcta taacgataaa cgggaatcag
    18481 aatccgcgcc gctgcctttt tcgctttcgg cgttgcagat tgaagcggct aaacgttttg
    18541 gtctgagcgc gcagaacgtg cttgatatct gccagaagct gtacgaaacg tacaagctaa
    18601 tcacttatcc gcgttccgat tgccgctatt tgccagaaga acattttgcc ggacgccacg
    18661 cggtgatgaa tgctatcagc gttcatgcac cggatctgtt gccgcagcca gtggtagatc
    18721 cagatatacg caaccgctgt tgggatgaca aaaaggtcga tgcgcaccac gccatcattc
    18781 cgaccgcacg gagttctgcg atcaacctga cggagaacga agcgaaggtc tataacctga
    18841 ttgcccgtca gtacttgatg cagttctgcc cggatgcggt gttccgcaag tgtgttatcg
    18901 aactggacat tgccaaaggc aaatttgtcg ctaaagcccg ttttcttgct gaagcaggct
    18961 ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa cgacggcacg ccgctgcctg
    19021 tggtggcgaa aggcgatgaa ttgttgtgcg aaaaaggcga agtggtagag cggcaaaccc
    19081 agccgccgcg ccattttacc gatgcaacac tgctttcggc gatgaccggg atcgcgcgct
    19141 ttgtgcagga caaagatctg aaaaagatcc ttcgtgcgac cgatggtctg gggacagaag
    19201 caacgcgtgc cgggattatt gaactgttgt tcaagcgggg tttcctgacc aaaaaagggc
    19261 gctatatcca ctccaccgac gccggaaaag cgctattcca ttcgctgcca gaaatggcga
    19321 cgcgaccgga catgaccgcg cactgggaat cggtgctgac gcaaatcagc gaaaagcagt
    19381 gtcgctatca ggactttatg cagccgctgg tggggacatt gtaccagttg attgatcagg
    19441 caaagcgcac gtc
//
LOCUS       DAOFSV010000081        19375 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.081, whole genome shotgun sequence.
ACCESSION   DAOFSV010000081 DAOFSV010000000
VERSION     DAOFSV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.081"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            92..673
                     /gene="sodB"
                     /locus_tag="QBH27_002959"
     CDS             92..673
                     /gene="sodB"
                     /locus_tag="QBH27_002959"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDQ7015246.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKPLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(819..1988)
                     /locus_tag="QBH27_002960"
     CDS             complement(819..1988)
                     /locus_tag="QBH27_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015247.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFLSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAIVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(2154..2243)
                     /gene="ynhF"
                     /locus_tag="QBH27_002961"
     CDS             complement(2154..2243)
                     /gene="ynhF"
                     /locus_tag="QBH27_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDQ7015248.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            2542..3567
                     /gene="purR"
                     /locus_tag="QBH27_002962"
     CDS             2542..3567
                     /gene="purR"
                     /locus_tag="QBH27_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDQ7015249.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(3564..4496)
                     /locus_tag="QBH27_002963"
     CDS             complement(3564..4496)
                     /locus_tag="QBH27_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7015250.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALKLENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            4609..5820
                     /locus_tag="QBH27_002964"
     CDS             4609..5820
                     /locus_tag="QBH27_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDQ7015251.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            6111..7259
                     /gene="cfa"
                     /locus_tag="QBH27_002965"
     CDS             6111..7259
                     /gene="cfa"
                     /locus_tag="QBH27_002965"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDQ7015252.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(7299..7940)
                     /gene="ribE"
                     /locus_tag="QBH27_002966"
     CDS             complement(7299..7940)
                     /gene="ribE"
                     /locus_tag="QBH27_002966"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDQ7015253.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            8155..9528
                     /gene="mdtK"
                     /locus_tag="QBH27_002967"
     CDS             8155..9528
                     /gene="mdtK"
                     /locus_tag="QBH27_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDQ7015254.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(9569..10825)
                     /locus_tag="QBH27_002968"
     CDS             complement(9569..10825)
                     /locus_tag="QBH27_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDQ7015255.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            11133..11209
                     /locus_tag="QBH27_002969"
     tRNA            11133..11209
                     /locus_tag="QBH27_002969"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11167..11169,aa:Val,seq:gac)
     gene            11214..11290
                     /locus_tag="QBH27_002970"
     tRNA            11214..11290
                     /locus_tag="QBH27_002970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11248..11250,aa:Val,seq:gac)
     gene            11399..11704
                     /locus_tag="QBH27_002971"
     CDS             11399..11704
                     /locus_tag="QBH27_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDQ7015256.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            11830..13434
                     /locus_tag="QBH27_002972"
     CDS             11830..13434
                     /locus_tag="QBH27_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDQ7015257.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFDQADEAGIGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLNLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNANEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPNVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGITA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(13446..14258)
                     /locus_tag="QBH27_002973"
     CDS             complement(13446..14258)
                     /locus_tag="QBH27_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015258.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFVRSDDHARLGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSGRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(14262..15047)
                     /locus_tag="QBH27_002974"
     CDS             complement(14262..15047)
                     /locus_tag="QBH27_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDQ7015259.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHSERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(15044..15712)
                     /locus_tag="QBH27_002975"
     CDS             complement(15044..15712)
                     /locus_tag="QBH27_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDQ7015260.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGVCPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(15776..16414)
                     /locus_tag="QBH27_002976"
     CDS             complement(15776..16414)
                     /locus_tag="QBH27_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDQ7015261.1"
                     /translation="MNHQDELPLAKVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(16427..18529)
                     /locus_tag="QBH27_002977"
     CDS             complement(16427..18529)
                     /locus_tag="QBH27_002977"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDQ7015262.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(18550..19176)
                     /locus_tag="QBH27_002978"
     CDS             complement(18550..19176)
                     /locus_tag="QBH27_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDQ7015263.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKESQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         4806 a         5052 c         4533 g         4984 t
ORIGIN      
        1 atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag gctattgtac
       61 gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg cactaccata
      121 tgctaaagat gctctggcac cgcacatttc tgcggaaacc atcgagtatc actacggcaa
      181 acatcatcag acttatgtca ctaacctgaa caacctgatc aaaggtaccg cgtttgaagg
      241 taaaccactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca acgcagctca
      301 ggtctggaac catactttct actggaactg cctggcaccg aacgcgggtg gcgaaccgac
      361 tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt tcaaagctca
      421 gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg tgaaaaacag
      481 cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccactga ccaccgatgc
      541 gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact atcgcaatgc
      601 acgccctggc tatctggagc acttctgggc gctggtgaac tgggaattcg tagcgaaaaa
      661 tctcgctgca taataactga tggcaaatgc aacattacct gatgcgcttc gcttaccatg
      721 ccaacgcgga tgaggcgtta acagcgcatt cggcaagata aatcgcacgt tgccagcaac
      781 tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttggc aacgcaaact
      841 gcttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc
      901 gcccccatca ccggcacaaa actgtatccc agcccagcgg aaattaccgc accaccagca
      961 gctgctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagacct
     1021 ggcgcttcac tggcgacacg catcacgcgc atctgtaacg gcggtacgat cgcaaaggtt
     1081 gcagctcccc acaccaccat gctaatagct gcgccgaact cattgcgggc caggaacggg
     1141 attgccagca taatcaccat cagcaacaac aaaaagcctt tcaacgtgcc gttaactgaa
     1201 cgatctgcca gtttgccgcc gagatagtta ccgatagaga atccgacacc aatcagcacc
     1261 agcattgccg tgacgaacac cggcgttacg tgggtaatgc tttgcagtac cggagagata
     1321 taggtgtaga gggtaaacat tgcaccagcg cccagtaccg tcgtcagcaa tgcagacagc
     1381 acctgcggac gcattaatac cgccagctct tttttcactt caggtcgcgc ccctgcgcca
     1441 cctttaggta atgagaggaa cagacttacc attgaaatca cgcccagccc cgctgttgcc
     1501 agaaatgaca tccgccagcc gatggtttcg cccaaccagg tcgccgccgg cacgccaccg
     1561 atatttgcca gggttaaccc cataaacata gtggcaactg cgctggcctg tttatgtttt
     1621 ggcactacgc ttgcggccac gactgaaccc aaaccaaaaa atgccccgtg attcaggctg
     1681 gtcaaaatgc gtgaaagcat cagggtcata taatccggcg cgatggcgga aagtacgttg
     1741 ccaagcgtga aaatcgccat caggaaaatc aacgcactgc ggcgggcacg atgagaaagt
     1801 agaagcgtca tcagcggcgc gccaaccatt acgccaactg cataagcact gattaacatt
     1861 ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa gcccattggc
     1921 gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa cggatagtta
     1981 attttcatgc cttatctcca cctcttcgcg tcattacgcg atattcatta aagtggcgaa
     2041 agcatgacag taatcacaaa aaaatgaaaa taacaaaaag agaaaacact tttgccattt
     2101 tgctaacaaa caggaaggag atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc
     2161 agcgcggcag tcaaacccac ggccacgatc aaaccgagga cgataatcgt tgttaccagt
     2221 gaaaatttaa ggtcggtgct catcaagttt tctccttttt tattaccaca caaaaagtga
     2281 tattacgcat ttttacacac tgtgatgaaa aaatctcccg tcatttataa tgataagtgt
     2341 ttttactact tccccttttc gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt
     2401 actggccatt gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc gcaacatttt
     2461 tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc gaatttgcag gagctgaagt
     2521 tagggtctgg agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt
     2581 tccactacaa ctgtgtcaca cgtgatcaac aaaacacgtt tcgtcgctga agaaacgcgc
     2641 aatgccgtgt gggcagcaat caaagaatta cactactccc ctagcgcggt ggcgcgtagc
     2701 ctgaaggtta accacaccaa gtctatcggt ttgctggcga ccagcagcga agcggcctat
     2761 tttgccgaga tcattgaagc ggtcgaaaaa aattgcttcc agaaaggtta caccctgatt
     2821 ctgggcaatg cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa
     2881 aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg
     2941 ctggaagagt atcgccatat cccaatggtg gtgatggact ggggtgaagc aaaagctgac
     3001 ttcaccgatg cggtcattga taacgcgttc gaaggcggct acatggccgg gcgttatctg
     3061 attgaacgcg gtcatcgcga aatcggcgtt atccctggcc cgctggaacg taacaccggc
     3121 gcaggccgtc ttgctggttt tatgaaggcg atggaagaag cgatgatcaa agtgccggaa
     3181 agctggattg tgcagggtga ctttgaacct gaatccggtt atcgcgccat gcagcaaatc
     3241 ctgtcgcagt cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc
     3301 gcactttgtg ctgctgatga aatgggtctg cgcgtgccgc aggatgtttc gctgatcggt
     3361 tatgataacg tgcgcaacgc ccgctatttt acgccggcgc tgaccacgat ccatcagcca
     3421 aaagattcgc tgggcgaaac agcgttcaac atgctgttgg atcgtatcgt caacaaacgt
     3481 gaagaaccgc agtccattga agtgcatccg cggttgattg aacgccgctc cgtggctgac
     3541 ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg gagtctcttc cggctcccgc
     3601 agccactcct tattcagcgt ctcactatcg ccgagatact caagcaacca ggttaacgca
     3661 ggcgacatat cattttgctg ccatgtcaga caacatgccg aatccgggaa ggggttttcc
     3721 agttttagtg ctacccactt cccctcatta agccacggtt tggcgaaatg tgttggcacc
     3781 atccctatgc ataatcctgc cgagatacag gttgccgatg attcccagtc aggcacgaca
     3841 agtctttttt ggttatccag caaccaggta atacgtttag gtagcgttcg cgaggtgtct
     3901 tcgcgcacca atgacggcca gttgcgcaac gtatcatcgc tgaacgggcc atccatcaac
     3961 gccagcgggt ggtggctggc aacaacgcaa ctccagctta acatccccat atcccggaag
     4021 gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa tcgccagttc cacgcgcccg
     4081 tcggaaagtg catcccagac accgttgaac acttcctgaa agacaaggag ttcgacatca
     4141 tcaaaatggc gataaaaatc aacgatcatc tgccgtgttc tctctggcct gacaatatta
     4201 tccactgcga tagctaactg accgcgccag ccgttcgcta tctgctgaca ttgctggcgg
     4261 gtgatctgca tttttttgac aacagagcgc ccttctttga gaaaccacgc cccggctgcg
     4321 gtcagttcca catcacggtg acgtcgttca aacagcggca ccgccagcca ctcttccagc
     4381 tgacgcacgg tatagctgac cgcagaagga acgcgatgca actcctgggc tgcggcgcta
     4441 aaactaccat tacgcgctac cgcatcaaca acttcgagag aatattctga ccacatagtc
     4501 tgcctgcaaa atttttgaaa ccaatcatca aatattaccg tttcacaaca ctaatttcac
     4561 tccctacact ttgcggcggt gtttaattga gagatttaga gaatatacat gcaacctggg
     4621 aaaagatttt tagtctggct ggcgggtttg agcgtactcg gttttctggc aaccgatatg
     4681 tatctgcctg ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt
     4741 gccagcctta gtctgttcct tgccggtttt gccgcagccc agcttctgtg ggggccactc
     4801 tccgaccgtt atggtcgcaa accggtgtta ttcatcggcc tgacaatttt tgcgttaggt
     4861 agtctgggga tgctgtgggt ggaaaacgcc gccacgctgc tgatattgcg ttttgtacag
     4921 gctgtgggtg tctgcgccgc ggcggttatc tggcaagcat tagtgacaga ttattatcct
     4981 tcacagaaag ttaaccgtat ttttgcgacc atcatgccgc tggtgggtct atctccggca
     5041 ctggctcctc tgttaggaag ctggctgctg gtccattttt cctggcaggc gattttcgcc
     5101 accctgtttg ccattaccgt ggtgctgatt ctgcctattt tctggctcaa acccacgacg
     5161 aaggcccgta acaatagtca ggatggtctg acctttaccg acctgctacg ttctaaaacc
     5221 tatcgcggca acgtgctgat atacgcagcc tgttcagcca gtttttttgc atggctgacc
     5281 ggttcaccgt tcatccttag tgaaatgggt tacagcccgg cagttattgg tttaagttat
     5341 gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct gtcgcgccgc gctgcagaaa
     5401 tggcaaggca agcagttatt accgtggttg ctggtactgt ttgctgtcag cgtcattgcg
     5461 acctgggctg cgggcttcat tagccatgtg tcgttggtcg aaatcctgat cccattctgt
     5521 gtgatggcga ttgccaacgg cgcgatctac ccgattgttg ttgcccaggc gctgcgtccc
     5581 ttcccacatg caaccggtcg cgccgcagcg ttgcagaaca ctctgcaact gggtctgtgc
     5641 ttcctcgcaa gtctggtagt ttcctggctt atcagtatca gtacgccatt gctcaccacc
     5701 accagcgtga tgttatcaac agtagtgctg gtcgctctgg gttacatgat gcaacgttgt
     5761 gaagaagttg gctgccagaa tcatggcaat gccgaagtcg ctcatagtga atcacactga
     5821 tctatatcga tatacttata cttaggctgc taacaaaatt ttgttgtatg attgaaatta
     5881 gcggcctata ctaatttcga gttgttaaaa ctacgataaa tattatgtta ttacggggac
     5941 aggatcgttc ccgactcact atggatggtc atttcggcaa gggttcctcc tttccctctg
     6001 ttctacgtcg gattatagac tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa
     6061 gcgtctacgc tgttttaagg ttctgatcac cgaccagtga tggagaaact atgagttcat
     6121 cgtgtataga agaagtcagt gtaccagatg acaactggta ccgtatcgcc aacgaattac
     6181 ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt gtcaaaaacc
     6241 ccgatttttt taaacgcgtt ctgcaggaag gatcgttggg gttaggcgaa agttatatgg
     6301 atggctggtg ggaatgtgac cgactggata tgttttttag caaagtctta cgcgcaggtc
     6361 tcgagaacca acttccccat catttcaaag acacgctgcg tattgccggc gctcgtctct
     6421 tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa agagcattac gatttgggta
     6481 atgacttgtt cagccgcatg cttgacccct tcatgcaata ttcctgtgct tactggaaag
     6541 atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc
     6601 agttaaaacc agggatgcgc gtactggata ttggctgtgg ctggggcgga ctggcgcact
     6661 acatggcgtc taattatgac gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa
     6721 aaatggctca ggaacgctgt gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg
     6781 acctgaacga ccagtttgat cgtattgttt ctgtggggat gttcgaacac gtcggaccga
     6841 aaaattacga tacctatttt gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc
     6901 tgctccatac catcggttcg aaaaaaaccg atctgaatgt tgatccctgg attaataaat
     6961 atatttttcc gaacggttgc ctgccctctg tacgccagat agctcagtcc agcgaacccc
     7021 actttgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt
     7081 ggtatgaacg attcctcgcc gcatggccag aaattgcgga taactacagt gaacgcttta
     7141 aacggatgtt tacctattat ctgaatgcct gtgcaggtgc tttccgcgcc cgtgatattc
     7201 agctctggca ggtcgtgttc tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa
     7261 ggctattcta tcgcccccgc tccgggggcg atttcagatc aggcttctgt acctggttga
     7321 ttcatggcat tttctcgtgc cgccagcaca cgttctaccg tatctaccac tgcctgagtt
     7381 tgtggatcga tttcaatgtt gacgcgtgcg ccgagttttt tcttcccaag agtcgtgcgt
     7441 tccagtgttt ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc
     7501 aggctaatac cgtcgatgcc aataaatcct ttgtacagga tatatttcat caactgacta
     7561 tcctggactt taaaccagat ctgacgatta ttttccgagg ttaatatttt cgccacttca
     7621 gcggtggtca taatatgacc tgacattaag tgtccgccaa tttcatcgct gaatttcgcc
     7681 gcacgctcaa cgtttaccca atcccccact tttaaatcac caagattagt aatgcgtaac
     7741 gtttctttca tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac ggtcaggcag
     7801 caaccgttat gcgccacgga cgcaccggtt tccagtccgt ccagcatgtg gtcgggtaac
     7861 tccaccacat gcgtgcgaaa atttggtttc tcatcaatcg acaccagttt tgcggtgccc
     7921 tgtacaatcc ccgtaaacat acttacaact cctgaaatca gttaagacat tctgttcagc
     7981 acaatagcag gtggaaaacg cccttaccag tgaaggggta agaatggcta ttttttcact
     8041 ggagaattaa taaatcctcg ctacaataga ctgaatttcc cctgcttctt ctttttgctg
     8101 cccattcagg cggcttttta gtctctcata taactacaaa taaaaggtgt tcacgtgcag
     8161 aagtatatca gtgaagcgcg tctgttatta gcattagcaa tcccggtgat tctcgcgcaa
     8221 atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta tagtgccacc
     8281 gacatggcgg cggttgctat cggaacttct atctggcttc cggcgatcct ctttggtcac
     8341 ggactgctgc tggcattaac gccggttatc gcgcaattaa atggttccgg tcgacgtgag
     8401 cgcattgcgc atcaggtgcg acaaggtttc tggctggcag gttttgtttc cgttctcatt
     8461 atgctggtac tgtggaatgc gggttacatt atccgctcca tggaaaacat cgatccggct
     8521 ctggcggaca aagccgtggg ttatctgcgt gcgttgctat ggggcgcgcc gggctatctt
     8581 ttcttccagg ttgcccgtaa ccagtgtgaa ggtctggcaa aaaccaagcc gggtatggtg
     8641 atgggcttta tcggcctgct ggtgaacatt ccggtgaact atatctttat ttatggtcat
     8701 ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc ggtgtattgg
     8761 gtcatgttcc ttgccatggt ttcttacatt aaacgcgccc gctcgatgcg cgatattcgt
     8821 aacgaaaaag gcaccgcgaa gcccgatcct gcggttatga aacgactgat tcaactcggt
     8881 ttgccaattg cgctggcact gttctttgaa gtaacgctgt ttgccgtcgt ggctctgtta
     8941 gtgtctccgc tcggtattgt tgatgtcgca ggacatcaga ttgccctgaa ctttagttca
     9001 ctgatgttcg tgcttcccat gtcgctggcg gcagcggtaa ccatccgcgt aggttatcgt
     9061 ctgggtcaag gctcaacgct ggatgcgcaa accgctgcgc ggaccgggct tatggtgggt
     9121 gtctgtatgg caaccctgac ggccatattc acggtttcac tgcgagagca aatcgccctg
     9181 ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc atttgatgct gctggcggcg
     9241 gtatatcaga tttctgactc tatccaggtg attggcagtg ggattttgcg tggttataaa
     9301 gatacacgtt ccattttcta tatcaccttt acggcttact gggtgctggg cttgccaagc
     9361 ggctatattc tggcactgac cgatctggtc gttgaaccta tggggccagc aggcttctgg
     9421 ataggcttta ttattggcct gacttcggca gccattatga tgatgctgcg tatgcggttc
     9481 ctgcaacgtc tgccgtcagc catcattctg caacgagcag ctcgctagta aagacaaggc
     9541 gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt
     9601 cttcgtctgg ctgtagtttc agtgcgttac acagagtttg ccgactggca tctgttatct
     9661 caacgtgtat atcaccagta acttcccctt tcacattgat gaagtcaggt gaaatttctt
     9721 tcgcactggt ttgcaggcaa atggctccct ctccgcgcag gcgtttcact ttcagttgca
     9781 catgcgttgc cgcatcagtg atcagccaca gttttccgcc atttagattg atgttaccaa
     9841 taccaaaagt gacactgtca ccttttaccg tactgccgtc gagaatcaat acaccgccct
     9901 gctgtacagt aatcgttgat tgttgcagat ctgcgccata gccaataatc attgaagcgc
     9961 cgtccagtaa tgcaacacta ccggtcagtt caaccggtgc gcgatcttcc tctacgacaa
    10021 aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt cccaccttgt attaccgcat
    10081 tttccagcgt accggcatgc acttcagccc gaccacccac attaacggac aggttttgcg
    10141 tcttaccgga actgtagagc tgaagaccat ccgtccccag acgatagatg gctccattct
    10201 caacaatagt gtcagagtct tcaccaccga gattattcat ggtgccacca gcaagcaacg
    10261 tatcactggc tttccccgcc agcataaaaa cgccgccctg attaatctca caattgcagg
    10321 cttccccacc tggatacacc aacaattcac caccttcatc taccacgacc gctgaggttg
    10381 tcccattgac caacaggcca ccctcttgat cgagaataat tttttccgcg cgatggcctt
    10441 ccagtacacg caggttaccg ccattttcca gcaacaaacc gcaggcataa cctttatcaa
    10501 cgctgaattc accttcggga tggcggccat taaccgtagc gagcgtagag agactaattg
    10561 cgcctccctc ggactgaaca atatctgtgg caatggcacc gtcgctgacc gatagtgtgc
    10621 cattgttacc gtactgtgtg catttggcta tcccaccggt tgccactttc aaccagccag
    10681 cccccgtgac cacggtattt tcggctcttc cgccagcttc aacaatcaac tcgccttcag
    10741 taagttgcgt cgcgccaata acctcgccgg tcttaacaat ttgcacgttc ccgtcgctca
    10801 tcaagttttt cgcatcagat cccatgattt attcctttgc tgcatctgtg tgcctttatt
    10861 gctacctaag tgtaaaggct acgaaggatt tatccacgac agatttgaga tggtggcaaa
    10921 caactctgtt taaactctga tacacggatt attgggttgt atcagatgta aatgcgatcc
    10981 tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca atttgcttaa aagctctgca
    11041 aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg ccaccttccc actctgccgc
    11101 taatattcgt ccccgttgtc acctacaacg ttgcgttcat agctcagttg gttagagcac
    11161 caccttgaca tggtgggggt cgttggttcg agtccaattg aacgcaccat cctgcgtccg
    11221 tagctcagtt ggttagagca ccaccttgac atggtggggg tcggtggttc gagtccactc
    11281 ggacgcacca gattttctta atctggtctt ctcctttttt ccctctgttt cttctctgta
    11341 tccaatacgt taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat
    11401 ggcaactttg ttacaacttc atttttcttt caatggccca tttggcgaca caatggctga
    11461 gcagcttaaa ccgcttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtttg
    11521 gacagaaagt gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag
    11581 cgcccttgcc tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt
    11641 tgtcgccaaa gttttcgatg tcaatgtgcc actgagtcaa atcaatcagg caaaacttgc
    11701 ctgacagatt ttaatcaagg gcggttagtg cccttttcat ccctgtctga aatttctcaa
    11761 attctaaaaa tctcaaccaa acttacctga taacactaaa ttcgaaagaa tgcgtacagg
    11821 taagtaacaa tgaaaacaat tgctattgtg ggtgccgggc ctacggggat ctacacctta
    11881 ttctcgcttc tacagcaaca aacgccactt tctatttcta tcttcgatca ggctgacgaa
    11941 gccggtatcg ggatgccata cagtgatgag gaaaactcaa aactgatgct ggcaaacatt
    12001 gccagtattg aaataccgcc gatttattgc acgtatctcg aatggctaca aaagcaagaa
    12061 gccagccatc tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta
    12121 ccgcgaattc tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca
    12181 cgacagcaaa aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt
    12241 acaaatgctg gcgtcatgct cgctacaaat ctgaatttac ccagcgagac gtttgattta
    12301 gcggtgatcg caacggggca cgtctggcct gatgaagaag aagcaaccag aacgtatttt
    12361 cccagcccgt ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg
    12421 ggaacatcct tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc
    12481 attgaagatg ataaacaaca cgtcactttt caccgcgata acgcaaatga aaagctaaat
    12541 attacgttaa tgtcgcgcac aggtatttta cccgaagccg atttctattg ccctattccc
    12601 tacgagccct tacacatcat caccgatcag gcattaaatg ctgagattca aaaaggcgaa
    12661 gtgggccttt tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca
    12721 gactggagcc aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg
    12781 tttgccgagc gcaaacaacg cgacccattt gactgggcag aagaaaatct ccaggaagtc
    12841 gaacgcaata aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat
    12901 gaagccgtac aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa
    12961 ggccttgccc gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcga
    13021 ctactggcct tacgtgaagc gggaatcatt catattctcg ccctcggtga agactacgaa
    13081 atggaaatta atgagtcgcg caccgtcctg aaaacggaag acaatagcta ctcgtttgac
    13141 gtttttattg atgcccgcgg gcagcgtccg cttaaagtga aagatattcc tttccctgga
    13201 ctacgcgaac aattacagaa aacaggggat gaaatcccta atgttggcga agattatacg
    13261 ttacagcaac ccgaagatat tcgtgggcgc gtagcgttcg gcgcgttgcc ctggttgatg
    13321 cacgaccagc ctttcgttca gggaattacg gcatgtgcag aaattggaga ggcgatggct
    13381 cgggcggtcg taaaacctgc atcccgtgcg cgtcggcgtc tttcgtttga ttaaaggtaa
    13441 agctatcagc actcaacaaa ctgatgttgt tcattaagac gatagcggac tccggctcga
    13501 atattgtttt caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc
    13561 agtgcggcgc atccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc
    13621 gttgaatttt caccactggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg
    13681 tttgcgccaa agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca
    13741 cccaatgtac aaacgcgtac tcgcattccg gtattcgcaa tccgaccgct atctccagcg
    13801 ctgcttatcc gcgaagaatt accggttaca gcaacccgtg cgcgtgcacc ggagctgcca
    13861 atgtgactgt taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc
    13921 gcggcatacc cagcggttgc gattctggcg ttatcctcac aacagccgag gcgagcatga
    13981 tcgtcgctgc ggacaaactg actgcaaaat agtcgttctc cgggatctgt ggccagtttt
    14041 tgcatagagg aaacttcctg atgcgcgaag ttttcatctg ccagccattt tttccacgca
    14101 tactcaacca gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata
    14161 tcggcataac taccgccacg tgggaaatgg cgcagaaacc agcgatacat caccgctccg
    14221 atacgccatt ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca
    14281 tcgaccatgc ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatga
    14341 ccgaagataa aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat
    14401 cttacgccag gaaacacatc tcccacggct tgcggataga gacacagcag ccccgtcaat
    14461 agtaacaatg gcaacaatcc atacatgaca ccaacatagg cgacctgctg taagggatta
    14521 aatttagact gggttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa
    14581 taaaatcgcg tttgttttgc cgctcgatcc agccaaccct gacgacgaat gcgatagtgg
    14641 tgaccattac ccccaacggc attgatcaga acaaagccaa gccagcatgc cagtaacaaa
    14701 aatccgcaaa cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccagcgca
    14761 aagtgattta tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc
    14821 cataatctga ccgctttgct gtaaagataa atcctctcac tatgatcggt ggctgatttc
    14881 ttcacgccac gagcacgaag caaggcatgt agccccagca ccaaccacat cccaaccagc
    14941 aatagtccgg caatgatcaa ccacaccggc caaaattggg gggtgaactg tggcacatag
    15001 ttcgccaact ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt
    15061 taatcaaatg ttgaccgaac cggcgataca gatgcggttt cccggcgcca ggtagctgat
    15121 attgatagta tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgtgaccaa
    15181 agatcaatgc gtgttccggg caggcactga cgcaaatggg cggaaagcct tttgccagtc
    15241 gggtctcagc gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa
    15301 cctggtacgg gcaaacgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc
    15361 gcacgatccc ctgctcatca cgccacgacg cccctgtcgg gcaaacgtcg atgcaaggag
    15421 catcttcgca gtgctgacat gactgacgaa aaaagtgata ctgcgttcca ttgtcgttat
    15481 cgatgactgg aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt
    15541 tacggcatgc gcgggcgcag atattacagc cattgcaccg tgactcgtca tgaatcatgg
    15601 cataccgaac ttccttttct tgcctcgtat tcgctaacag agaagacgca gaaccagtaa
    15661 aaaagattac tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat
    15721 cccctaatgc agtggctgca actgtccacc ctatccatga catccccctg aaatttcagg
    15781 ggttctgaaa ctgctctacc tcgaaccatt cgcataagcc atacgccttg ccataggtca
    15841 taaatcgctc gctaaacaag tacaacactg ccgtcgatga ctccacctgc cgtatatcgg
    15901 catattcagg tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt
    15961 aatatggcgc ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa
    16021 aacgaacggc gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg
    16081 ccgcgtagtt ttcactcatg gcctgcgttg agtaatagta atcatcctgg cttcccgtca
    16141 tacaagcgat atccgcgtag tctccgttct gcttaatgtt ttccagcaaa actgacagtt
    16201 cctcttctgc aaccgaatag ggtggcgaga gaaacacggc gcgcgccacc agctgcccag
    16261 ccgcagaatg ctggcgaatg aactctgcca ggatttcagc cggttctggt tcggttaccg
    16321 tatcaacggg gtgacgcatc ccctgcaacc attgccgctt cgcctcatca atttcgctga
    16381 ctttcgctaa cggcaattca tcctgatggt tcatctctcc cattccttac gcaggcagta
    16441 gattgtgtgc tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc
    16501 gggtcggaca accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag
    16561 catgcatatc gtcacggtcc attttgtctg taccttcagt aaagaccggg atctgcggat
    16621 ccttgtcgaa tacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca
    16681 tcagttttac cgtgtaggcg cgatgcaacg taaagatacg ctctgcagct aaatctaatt
    16741 tttcctgagt catatcttcg ccagtgatcg ctttgaagaa tttggcttca agcgccagat
    16801 caccccggta attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca
    16861 gggtgacggc gttatgcaaa cagacccgca acagcgacca cttagcatat ttaatttttg
    16921 cgtcattgat cggtgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagct
    16981 ctttcgccac ttcacgctgc agtttcaatg gcaagccgga accaataaaa ttgatatggg
    17041 tatgcgtcat gcagtcacgg ttgaacatac agttaacaat ggaaccgact tgcgctgacg
    17101 cttcattggc atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc
    17161 cccagtactc ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt
    17221 gactcaactc acccacgcga tgcgccagac ggtagtaaaa atctttaata aagttaacgt
    17281 cacccgcttc cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct
    17341 tgaacacacc tttgctgtaa caataagtaa agtcgcgatg caactgcccg tagttacacc
    17401 ataggccgta gtcatcgaac aaattcagac caaccaggtt accaatcaca cgaccatcgt
    17461 ccttatcttc aaaatctttc ggcccgttcg ggaagatggt ggtatggaca aagtttgcta
    17521 cacaagtgtt accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt
    17581 gggtcataca acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg
    17641 ccgccggacc taagtcaaaa acggatttat aggtacgaaa gccgaccgta ttctgattgc
    17701 ctggcggaat ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt
    17761 tacgtgctgt ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg
    17821 gcacgacatg gttgttattc gcaccgataa gttcagtcat catgtaatcg ttgagacgct
    17881 tcatctcctg acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca
    17941 ggtttttcga acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca
    18001 acatgccaga gagaggaaca aggttttctc cagcctgacc aatagccgcc acacaggttt
    18061 ccggactggt caagcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga
    18121 aatcggcttt ttcaaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg
    18181 ctttcccttc gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc
    18241 cccccatatg ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat
    18301 ttacgcgaga actacagggg gcaccagatc cagttaatgg gccggtagca aaaactaatt
    18361 tattcgcttc atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc
    18421 cgaagcccat gccaccgaca aaacttttaa atttactgga atcttcgagg gtaatatttc
    18481 ctgtcgtgag attgactctt aatatattac ctgtccaacc gttagccatg attttttcct
    18541 ttgcaagatt cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca
    18601 ggcgtttgcg cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac
    18661 ggtggccatc atccacgggc atgccgtagt acaggcgcta cagccaatac aacgtttatg
    18721 atcgacggta atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt
    18781 catgcattgc gactctttgc attgacggca ggtatccgcg gtatagttga gatcgccata
    18841 caggccaccg ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat
    18901 acgggagaag aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg
    18961 gtggcaaccc gtgcatcgcg cacgttgggt caccagcacc cctttgggtg tatttatcaa
    19021 ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaaagcagg
    19081 ggcaattgtt aaaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa
    19141 gccaatattt aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac
    19201 acagtgctga caattcaaca gcctgatatt aagcactcaa ctaatacgct gctgacgctg
    19261 tcctcaacac cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgctaac
    19321 aaactaatcc tttatggtta ggagaaaaaa taatgtgatt attctgggct cttaa
//
LOCUS       DAOFSV010000082        19011 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.082, whole genome shotgun sequence.
ACCESSION   DAOFSV010000082 DAOFSV010000000
VERSION     DAOFSV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.082"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..970)
                     /gene="cmoB"
                     /locus_tag="QBH27_002979"
     CDS             complement(<1..970)
                     /gene="cmoB"
                     /locus_tag="QBH27_002979"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDQ7015264.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPTQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(967..1710)
                     /gene="cmoA"
                     /locus_tag="QBH27_002980"
     CDS             complement(967..1710)
                     /gene="cmoA"
                     /locus_tag="QBH27_002980"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDQ7015265.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(1751..2146)
                     /locus_tag="QBH27_002981"
     CDS             complement(1751..2146)
                     /locus_tag="QBH27_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDQ7015266.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(2199..2969)
                     /locus_tag="QBH27_002982"
     CDS             complement(2199..2969)
                     /locus_tag="QBH27_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDQ7015267.1"
                     /translation="MKISKLILNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSEAIR
                     YAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(2951..4264)
                     /locus_tag="QBH27_002983"
     CDS             complement(2951..4264)
                     /locus_tag="QBH27_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDQ7015268.1"
                     /translation="MDKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRTGSFDYAAQFPDSPNLQAFGVSKKEITVKELEEKWLDLKRMEISAN
                     AFNRYESVARTMVPKIGGSRLVSSVTKEELLYIRKDLLTGYQNSTKNKAAAKGRSVVT
                     VNYYMTTIAGMFQFAADHGYLEANPFQGIKPLKRARAEPDPLTRDEFIRLIDACRHQQ
                     TKNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVV
                     HLIQPAISVLKNQAEMTRLGKQYNIKVQLREYGRSVNHECTFVFNPQVVRKSEQVGFV
                     YKVDSVGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVF
                     NVYGAWMTDSNAEQIAMLNQKLTDYVPMMPHSHQSDTRGLLKSVS"
     gene            complement(4320..4556)
                     /locus_tag="QBH27_002984"
     CDS             complement(4320..4556)
                     /locus_tag="QBH27_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDQ7015269.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRARKECWMVGREYI
                     HVSPDGNPKPSSECMYNRKAIDAWVASMKNKQPR"
     gene            complement(4565..4711)
                     /locus_tag="QBH27_002985"
     CDS             complement(4565..4711)
                     /locus_tag="QBH27_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015270.1"
                     /translation="MNKAFERWVHQRYCNRYDLTRDVDGFYCREVVKRMFEVWCHCRG
                     LSVV"
     gene            complement(4715..4957)
                     /locus_tag="QBH27_002986"
     CDS             complement(4715..4957)
                     /locus_tag="QBH27_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ7015271.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            complement(5042..5986)
                     /locus_tag="QBH27_002987"
     CDS             complement(5042..5986)
                     /locus_tag="QBH27_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDQ7015272.1"
                     /translation="MITITKGRLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     ADPVKRVNSDQMHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVFNGEYGDAYQGA
                     REDLSIWKRRALEADEHVRRLEQINDHMVKEAQGESRMGEPVIREPAPVVPEEATPEN
                     VEMLSGYVSTYKLTDSERDIAAEIWNACRAAMLQSGNFRENKNSSTNNFREIAETSTN
                     YPAIPSEVLSAILKVARIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEP
                     IAPNDVREQTAIPQVPVTPDGWISCSERMPDDRQEVNQ"
     gene            complement(6320..6498)
                     /locus_tag="QBH27_002988"
                     /pseudo
     CDS             complement(6320..6498)
                     /locus_tag="QBH27_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6500..7099)
                     /locus_tag="QBH27_002989"
     CDS             complement(6500..7099)
                     /locus_tag="QBH27_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDQ7015273.1"
                     /translation="MSNINYQVLREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAEANPATVLALLDEQERNQQYIKRRDQEN
                     EDIALTVGKLRVELEETKSKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYG
                     HPINADERQVMIPKENGCWLYLIDLEHALRVADIRIKGE"
     gene            complement(7096..7335)
                     /locus_tag="QBH27_002990"
     CDS             complement(7096..7335)
                     /locus_tag="QBH27_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015274.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRDMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRKSFFSCIAAQIRKGGNQ"
     gene            complement(7328..7531)
                     /locus_tag="QBH27_002991"
     CDS             complement(7328..7531)
                     /locus_tag="QBH27_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015275.1"
                     /translation="MSVIKTHTGIVITRDGPQVKKLHQTKRMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPIEVKHV"
     gene            complement(7528..7890)
                     /locus_tag="QBH27_002992"
     CDS             complement(7528..7890)
                     /locus_tag="QBH27_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015276.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWLWTFQVGDLE
                     NVNITHWAVMPKAPKNKK"
     gene            complement(7881..8417)
                     /gene="yfdR"
                     /locus_tag="QBH27_002993"
     CDS             complement(7881..8417)
                     /gene="yfdR"
                     /locus_tag="QBH27_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDQ7015277.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFADHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDSSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(8508..9425)
                     /gene="rdgC"
                     /locus_tag="QBH27_002994"
     CDS             complement(8508..9425)
                     /gene="rdgC"
                     /locus_tag="QBH27_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDQ7015278.1"
                     /translation="MKLPKFRNAIVYRATLPSIQAIEGHLLELPYSEIGETEFSRSSF
                     VENPVTGELVTPISGGYAMVIRHDQKIIPRHVVMKEANSRIQTIENMSGNKLKRAERL
                     AIIDNVRVDLCKQAFVKSTLILALYSTDEKLLVINTTNKIIAGMVCAMLIKVVGSVKT
                     ETINISDIKNGLTTRLNNYINGAANAFEGFTVGNYIQLSRYADQKEIIRYSAEHESIQ
                     SELADSLSSSFTADKMELSGCGVTFILTENFHFSRINTQSQTFNDEDDKAFQWRHQTG
                     ADLFQFSKVVNLMCDLLSYKEDKSQNPTT"
     gene            9980..10474
                     /locus_tag="QBH27_002995"
     CDS             9980..10474
                     /locus_tag="QBH27_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015279.1"
                     /translation="MTRDDYKFYVHYSYVLEKMNYTLLTRIDKLITLMLIVLGFSVFA
                     PYSNLFIFGLFVAVLSVLQLVYQFGQEAGMSKEQARHYKRLLIESDTLPEDQLQARYL
                     KIQDTDSNPWKSLERAAFIRACIFAGVPTKYDLTTKEKFIAWCAGDLPLENELTDIND
                     DQQA"
     gene            complement(10831..11457)
                     /locus_tag="QBH27_002996"
     CDS             complement(10831..11457)
                     /locus_tag="QBH27_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDQ7015280.1"
                     /translation="METVGQRIKALRRVTRTSQKELGKFCGVSDVAVGYWEKDINVPG
                     GEALSKLAKFFNTSIDYILYGAEFEGKLVTNMRRVPVISWVQAGQFTECRTAEVFSEV
                     DKWVDTSLKIGDNSFALEVKGDSMTNPNGLPTIPEGATVIVDPDAEPRHGKIVIARLD
                     GTNEATVKKLVIDGPQKFLVPLNPRYPNIPINGNCLIIGVVKGVQYEL"
     gene            11555..11755
                     /locus_tag="QBH27_002997"
     CDS             11555..11755
                     /locus_tag="QBH27_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDQ7015281.1"
                     /translation="MTTDDIESYFGSIEKVAAFFGITTEAVYQWRNRPGQLIPKGRAA
                     EAAYRTCGRLPFKPELYEKSNG"
     gene            11793..12377
                     /locus_tag="QBH27_002998"
     CDS             11793..12377
                     /locus_tag="QBH27_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ7015282.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWAMVLQDASGTRHVTDAIARRSNGVFVPLVVIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDQAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPEKSNARECAAPGVVASIASGCGETNA"
     gene            12553..12744
                     /locus_tag="QBH27_002999"
     CDS             12553..12744
                     /locus_tag="QBH27_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ7015283.1"
                     /translation="MRQVNRWFKDHHGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     HFRQKFREIRDDHEQTSND"
     gene            12722..13579
                     /locus_tag="QBH27_003000"
     CDS             12722..13579
                     /locus_tag="QBH27_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDQ7015284.1"
                     /translation="MSKPVMIDSDNSGRYIVLAAAKSVAGIGVLKLITEHNRAHAVFS
                     CHEHCYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSI
                     LVRQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQMEQALIGKVDT
                     SCVAHNAHVVYLYMAEIHRVWLEQLYPMLVSVQSPLASSLYDYINDGVFVAGLVDSEL
                     NEKHKEVRK"
     gene            13576..14517
                     /locus_tag="QBH27_003001"
     CDS             13576..14517
                     /locus_tag="QBH27_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7015285.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKACFHPSESGGDPSVKSKHDPSDKKPSRPDALQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTHTEKREDPYFKASYDNVDYSQIPEGFRG"
     gene            14514..15008
                     /locus_tag="QBH27_003002"
     CDS             14514..15008
                     /locus_tag="QBH27_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDQ7015286.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGKTRRHFPHQAEISQEEKLQPVLETIPVSNFYVGTNDPWVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLQKSSKRAASGEEWYLSGN
                     YVGA"
     gene            15008..15661
                     /locus_tag="QBH27_003003"
     CDS             15008..15661
                     /locus_tag="QBH27_003003"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDQ7015287.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGAAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            15658..16047
                     /locus_tag="QBH27_003004"
     CDS             15658..16047
                     /locus_tag="QBH27_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ7015288.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            16067..16876
                     /locus_tag="QBH27_003005"
     CDS             16067..16876
                     /locus_tag="QBH27_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDQ7015289.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPDKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            16884..17873
                     /locus_tag="QBH27_003006"
     CDS             16884..17873
                     /locus_tag="QBH27_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ7015290.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPTVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            17891..18271
                     /locus_tag="QBH27_003007"
     CDS             17891..18271
                     /locus_tag="QBH27_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HDQ7015291.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            18308..18700
                     /locus_tag="QBH27_003008"
     CDS             18308..18700
                     /locus_tag="QBH27_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDQ7015292.1"
                     /translation="MLRVVTSPHSLNPPRAGFLWLNVKCAGGCECRLRRYFGVTAQVP
                     YTDVWTHKPVQFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGSGSTVKAALALG
                     RRAIGVELETERFEQTVREVQDLVSQNG"
     gene            18741..18816
                     /locus_tag="QBH27_003009"
     tRNA            18741..18816
                     /locus_tag="QBH27_003009"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18774..18776,aa:Ile,seq:cat)
     gene            18824..18900
                     /locus_tag="QBH27_003010"
     tRNA            18824..18900
                     /locus_tag="QBH27_003010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18858..18860,aa:Arg,seq:tcg)
     gene            18914..18990
                     /locus_tag="QBH27_003011"
     tRNA            18914..18990
                     /locus_tag="QBH27_003011"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18948..18950,aa:Arg,seq:tct)
BASE COUNT         4737 a         4594 c         4665 g         5015 t
ORIGIN      
        1 acggcttgcg cgcaatcagc accgcgcgtt taggcgcagg ataaccttcc accgttttac
       61 ccggatcatg cgggtcgaga aaatcggcca gagactcggt gaccatccat tcggtgcgtc
      121 gctgctcttc tgtggtggta acgctcacat ccgcaatgcg aatatcaaca aaaccacact
      181 tcttcagcca gtttttcagc gccagcgcgg aaggaatgaa atagacatta cgcatttgtg
      241 cgtaacgatc gcccggcacc agcaccgtgt tctcatcgcc atcaataacc agcgtttcca
      301 gcaccagttc gccttcattc accagttggt cttttaactg ccagagatgc tccagcggtg
      361 aacgacgatg ataaagcacg cccatcgaaa agacggtatc aaaggctttc agtgccggaa
      421 gttgttcaat acctaacggt aacaaatgtg cgcgctgatc gttacccagt agtttacgca
      481 ccgcttcaaa ctggcacaga aacagttgtg tagggtcgat ccccaccgcg aggtgcgcgc
      541 cagcgccaat catgcgccac atgtgataac cgctgccgca gccgacatca agaatggtgc
      601 gcccggttaa atcagaaaga tggggcagaa cgcgatccca tttccagtcg gaacgccatt
      661 cggtatcgat gttgacgcca tacagtgaga acggcccttt gcgccacggc atcaggttgc
      721 gcatcagcgt ttcaatgcgc ttaatttgcc cggcgctcag tggctcttcg ctttcggcgg
      781 ttacgctatg caataaatcc agacgatacg gtttaatttc aggcagaaat tccaccgcgt
      841 tggaccactg cttaaacagc ccgtgctgct gctcgcgctg ccagttagca atctgcgtgg
      901 gcagcgtttc gagccagtgt gaaagatgat ttttggcaat cagagaataa aagttaccaa
      961 agtcgatcat gcagcgtcct ctgcttttaa tgccaccagt gaaccaaagt taaagcactg
     1021 gaaccacagc tcgctatgct caaaaccggc tttatgcagg cgtgctttat gggtttccac
     1081 ggaatcggtc agcatcacgt tttccagcat gctgcgtttc tggctgatct ccagttcgct
     1141 gtaaccgttg gcacgtttaa agtcgtggtg catgttgaac agcagttcac caactttggc
     1201 atcttcgaaa ctgaattttt ccgaaagcac cagcgcgccg cccgggttca gtccttgata
     1261 aattttatcc agtaacgcct ggcgctcgga aggttccagg aattgcaggg taaaattcag
     1321 caccaccatc gatgcgtttt caatggcgat atcgcgaata tcaccttcaa taacgtctac
     1381 tggcgtagga gctttatagg cgtcaatatg acgacggcag cgttcaatca tcgccgggga
     1441 gttatcgatg gcaataattt tgcaattatc atgatgaatg ttgcgacgca ccgagagtgt
     1501 cgccgcgccc agagaacaac ccagatcgta aacctgcgta ccaggttgaa cgaagcgctc
     1561 ggctaacata ccaatcatgg aaataatatt ggaatagccg ggaacggaac gctggatcat
     1621 atccgggaag acttcagcta cccgttcatc aaaggtccag tcgcccagtc tggcgatagg
     1681 ggcagaaaat agcgtgtcgc ggtgagacat aacgtaaaaa tccgggaaaa agaaagtggc
     1741 gtattgtgcg ctaacgcagg gagaaaacca actcccaggg catataccaa agattcgcca
     1801 gcaccatcag caacagcgca caccaggtgg cgctcatgcc agaacgtcgc cagcggaaca
     1861 gacggtgatg aaaaccgtaa taatgcatca gacgaccagc aagcaaaacg atgccgcaaa
     1921 tatgcaccat ccaggtttct gcgccattca tttccataaa cagcatcaac acaatcgcga
     1981 tgggaatata ttccaccgcg ttaccatgaa tgcgaatagc gctttgcagt tcgctaaaac
     2041 cgccgtcgcc ataggcaacg cggtactgca ttcgcaggcg aacgacatca aaagagaact
     2101 tcattaataa caacgcactt aaaacggcat acagcgcgct taccatacaa actcccttta
     2161 aaatggccga tggcacctgt ctatgatagg tggcaaattc agaaaagaga agattgctgt
     2221 ggaatagccg gaactgctcc gatctctgga agcgttttac gtaagtcttc ccacagggta
     2281 tgtaccagtt ccggggcctg ggcaatatct ggcgtatgta aaaaaagata aggcgtagtg
     2341 gtctgatgcc actgcgctaa tttttgtaac cagacctgaa ataattcccg gttttgcgtc
     2401 atatcatcac taccgataaa acggatcagt ggatttttcg ccgtcagtac agcatgtacc
     2461 ggaactttag gtttttttcg ttgagcgtag cgaatagctt cactgtgtgg acgtgctgca
     2521 tgaaccgggc ggctgtctaa aatcacccga ttaacgccgc gctgatgtaa accgcgatta
     2581 agcgtttgtt cctcttcccc tttggcgaaa aactgtggat ggcggacttc cacaccataa
     2641 ttaaattcgc cgggaagaga atcgagaaaa tgccaaagcg caggcagctc ccgtgggccg
     2701 aatgtggcag gcagttgcag ccagtattgt ccaatgcgcg gagccaacgg tgacatgcgg
     2761 gtcaaaaatt cagtcactaa atcatcgcaa tgccgtaatg ctgcctgatg cgaaatagtc
     2821 gccggaaact taaaacagaa gcggaagtca tctgtggtct gctcacgcca gcgcaggaca
     2881 acctcgggtt tcggcagggc ataaagcgtg gtgttaccct ccacacagtt aacatgacgg
     2941 gtattaagga ttaacttact gattttaata agcctctggt gtcactttgg tgactatggg
     3001 gcatcattgg gacataatct gtcagcttct gattcagcat tgcgatctgt tctgcattgc
     3061 tgtcagtcat ccatgctccg tatacattga ataccatctg ggcacttgca tggcccatct
     3121 ggctggcaat gaagcttggg tttgctccgg cagataatga ccagcacgca taagtgtgtc
     3181 gtgactggta tgcctttcga tgcctgatcc ctgcacgctt aatggctgtt tcccatgagt
     3241 cacctacaga atcgactttg tagacaaaac ctacctgttc gctttttcta accacttgag
     3301 ggttaaacac gaaagtacat tcatggttca ctgaacgtcc atattcacgt agttgcacct
     3361 tgatgttgta ctgcttaccc agtcttgtca tttcagcctg atttttcagg acactgatag
     3421 cgggctggat aaggtgcaca accctgtttg tgcttgcttc agttttcggt agagtgaact
     3481 caccaagttt cgtataattg cgcctgatgg taattgttcc tgccttcaga tcgatatctt
     3541 cccaggccag ggagaccagt tcaccgtgac gcattcctgt gtacacagcc aatgaccaca
     3601 ggtttttcgt ctgctgatgt cggcaagcat ctatcaggcg aataaattcg tcacgagtta
     3661 gcggatctgg ctctgccctg gctcttttaa gaggcttaat tccctggaag ggatttgctt
     3721 ctaagtaacc gtgatctgca gcaaactgaa acattccagc gattgtcgtc atgtaataat
     3781 ttacagtaac gacgctccgt ccttttgctg ctgctttgtt tttcgttgaa ttctgatacc
     3841 cggtcagcaa atctttcctg atatacagca attcctcttt ggttaccgat gacaccagtc
     3901 tactgcctcc aattttcgga accatcgttc ttgcaacgga ttcatagcga ttgaatgcat
     3961 ttgcagagat ttccattcgt ttcagatcca gccacttttc ttcaagttcc ttcaccgtaa
     4021 tttctttttt acttacccca aaagcctgaa ggttggggga gtcagggaac tgtgcagcat
     4081 aatcaaagct tcctgtgcgg atggcaaaac atactgatgt ccgcagttcc ccggcgatct
     4141 tcctgttctt ggcagtgtca gggacaccaa gattttccct gacacgttta cctttaaaat
     4201 taaaccagat gcgtaatgtg ccgccgtggt tttcgacgcc tgttggatat ttgactttat
     4261 ccatcgatac ctccagacgc ccaagagcga tacgagctta catatttcat gatattaaat
     4321 cacctgggtt gtttgttttt cattgaggcg acccaggcat ctattgcttt tctgttatac
     4381 atacattcac tggaaggctt tggattaccg tctggtgata cgtgaatata ctctcttcca
     4441 accatccagc attctttccg ggcccgaaga attgtgcctg gtttgagccc ggtaattgcg
     4501 ataagaacgc tttcacaaac ccattcattg ggagccagtt gaatcacatt gcccatgtat
     4561 tacctcacac aacactcagc ccacggcagt ggcaccacac ttcaaacatt cgcttcacaa
     4621 cttcacgaca gtagaagccg tcaacatctc gcgtcaggtc atagcgattg cagtaacgct
     4681 ggtggaccca tcgttcaaat gctttattca ttctttactt cctttttatg gctcgtaatt
     4741 ttttcaggtg cttttcctgc tcagtgtccg cgagaatttt gcggtactcc tggtggtcaa
     4801 tatgttcgaa caggcagttt aactcaccaa tgcgtacccg cccggatcgt ccgtccatcc
     4861 gtcgaaagaa cactgagtgc tcagtgatgc gagtaatcac cacggggtat ccagctctgt
     4921 ccgtgtatat ctgaccgcgt tgaatcaaag cgaacatgtg gttatcccca tcgacaaatc
     4981 gagaacacaa caaacgctgc tgcgaatacc acccccagag ttacgattgc atcaggccag
     5041 ctcattgatt cacctcctgc ctgtcgtccg gcattcgctc actacagctt atccaaccat
     5101 ccggagttac cggaacttgt ggaatggctg tctgctctcg aacgtcatta ggcgctatag
     5161 gttctgctgc caactgactg gcatatttgt taatggtaac gataagctct tgctcagcct
     5221 catccagaca atcaccgata cctcgcctgt caccgtcaaa atcatcgaaa tcggcacgaa
     5281 tcctggcaac cttcaggatt gcggacaaca cctcactagg aattgccgga tagttggttg
     5341 acgtttccgc gatttcccga aaattattgg ttgacgaatt cttgttttcc cgaaagtttc
     5401 cggactgaag catggcggcg cggcaggcgt tccatatttc ggcagcaata tcgcgctcgc
     5461 tatcggttaa tttgtacgtt gaaacatagc cagagagcat ttctacgttt tccggagttg
     5521 cttcttcagg cactaccggt gctggctcac gtattacagg ctcgcccatg cgtgattctc
     5581 cctgcgcctc tttcaccatg tggtcattga tttgctccag tcgccgaacg tgctcatcag
     5641 cttcaagcgc tcgccgtttc cagatggaca ggtcttcacg agcgccctga tatgcgtcac
     5701 cgtattcgcc gttaaatact ggcgctggcg gggcgatgcg tccaagcaac ttatttacct
     5761 ctttcgccat cgcgtcatat ttatctaaat agcgattagc ttctaagcag actcggtgca
     5821 tctgatctga gttaactcgt ttaactggat ctgcttccaa tgatgccagt gcaatccgtg
     5881 ccagttcttc cgcttcttct gctggcagta caacgttgct acccggtccg tatgtttcgc
     5941 gccactgctt gattgtcagc agtcgccctt tggtaatagt gatcatgccg cgtttccttc
     6001 tttcttatta acaatcacac cgtcatatat ttcattaagg tgccctctca actccatgcg
     6061 ccttaatgca gataacatgt aatcgcattc aacctgctta ttcccggtaa atggcttatc
     6121 gtcaggatta ccccaacagc aattacccct gggccatcca tgtactttcc gtactcttcc
     6181 gttaacaacg tgaagtaatc cccagccggg aggtaaatcc tcaactgaaa taatttccgg
     6241 ctcactaata aagaatcgcc agtcgcccat gccaagtgag ggatttttac ggaaacgctt
     6301 ttttctatct gccaacaagt cagcacgaga acacttcgcc tctatcaggc atgatgctga
     6361 atttctgaat cccatagcat ctggctgttc tccggtactg gttacagcaa caaagcggtc
     6421 atgaaagcaa accttgaacc cgttgcgctt aaggaacttg tacgcaatct gacagagttc
     6481 gtggtgtgtt aacgccatat cactctcctt tgatgcgaat gtcagcgacg cgtaatgcgt
     6541 gttctaggtc aatcaggtaa agccaacagc cattttcttt aggtatcatg acttgtcgct
     6601 catctgcatt tatcgggtgt ccatatcgaa ggtcgtagcg agtcggtaat tgaacttccc
     6661 gcgcttccag ttcagcaata cgcttgcacc catcagagat aactccctcg taatactcgc
     6721 gctgctcgtt gagttttgat tttgtctcct caagctcaac gcgcagcttc cctaccgtta
     6781 gcgcaatatc ctcgttctcc tggtcgcggc gtttgatgta ttgctggttt ctttcctgtt
     6841 catccagcag tgccagcacg gtagccgggt tagcctctgc tatgaattca gcgtttgcat
     6901 aagcctgagc atctgtttca accaggcagt taacgtgaca ttctgcaatt acgccaccgg
     6961 gttctccttt ccatttttga caaacaaaaa ctcctgttaa attgccgtgt tggttaacag
     7021 atgtatgccc tacgatgtag cttcctttag ttgctttctc tgccttttca cgcagtacct
     7081 gatagttaat gttgctcact ggttgcctcc tttacggatc tgcgctgcga tgcacgaaaa
     7141 aaaagacttt cgcgtatgac tgttaagagc tggcgcgaac gccgcgttaa gaacggcggc
     7201 atcacagccg tcatcgatat agagcgcaat ttttttctcc aggcgtgctt tggcttcctg
     7261 caactgcata tcccggcacg cacgcgggat atactcagca atttgagcga tagatttttc
     7321 gttctgttta aacatgcttc acctcgatag gcttgatggt atcgatcagc agtcggcggc
     7381 gagtattttc tgcaaagtgg cggcgtccgg tttctttgtg gtaaaactcg ttttttccga
     7441 cgacccacat ccgctttgtc tggtgcagtt tttttacctg cggaccgtct cgggtgataa
     7501 caattcctgt atgagttttt atcacgctca ttttttattc ttcggtgctt tcggcattac
     7561 tgcccagtga gtgatattga cgttttcaag gtccccgacc tgaaatgtcc acagccattc
     7621 tccggtttct ttttgtcccc aggtgtacca gagagaacgc cagccaatta gccagccttc
     7681 tccgttagca tcaaataaca gaacactttc atttgctggc ggcagttcag ctgacactgg
     7741 tattattttg ttttccagtg ccgcacattt agcttcaagc gcatcgaatt tacgtaccag
     7801 gtactcagca tttgtttcat tcactttcag atctcgtggt acacatttcc cgcgaagaaa
     7861 cccttccatt tcgaaaacat tcatgcgcat ttgcgtaact ccgataactc gttaaaacgt
     7921 tccataaaca tcccgtaggc atggcctggc gacagtggaa tcactttgaa catctctgtt
     7981 gccgggatac cttccagtac aggccagaaa gagctatcat caagcccgag atcgcggcgt
     8041 tcggttgcca gcatgatgag atcggcatat ttcacgggcg tgctcataac cgggggtaac
     8101 ccgtatttct cacggattac ggcgtctatt ttttcttcca tccgtttata gtcaggaaga
     8161 aggcgtttca gtggagcggg aatatcctgg caatacgctt ctgttgcatc atgcattaac
     8221 gcttcaaaag caaattcctg cggcaccagc tggctgcaaa gcaccgcatg ttgggcgaca
     8281 ctgtagaagt gagaaagatg atcggcaaag cggcagatat ttgaaaggga aaccgcgata
     8341 tcgttaatca cgatgtcgtc tttatttatc ctgtcataat aaaaatgctt cccggaaaaa
     8401 gttttaataa atgacatttt gttctccacg ttatatgcgc tgcaccgctc tgaattttga
     8461 ttgcagcaat ccagcccatt tgacatggga tgattgctgc aattttttta agttgttgga
     8521 ttttggcttt tatcttcttt gtaagaaaga aggtcacaca tcaaattaac taccttgctg
     8581 aattggaaaa ggtcagcgcc agtctgatga cgccactgga atgctttatc atcttcatca
     8641 ttaaatgtct gagattgagt attgatgcgc gaaaaatgga aattttcggt aagaatgaag
     8701 gtcacaccgc aaccggataa ctccatttta tcagcagtga aactactact aaggctatcg
     8761 gccagttcgc tttgaattga ctcatgctca gctgagtagc gaataatttc cttttgatct
     8821 gcgtagcgtg atagctgaat ataatttccg acagtgaatc cttcaaatgc attggctgca
     8881 ccattgatgt agttattcag gcgtgtagtc agtccattct tgatatcact gatgttgatt
     8941 gtttctgttt tcactgaacc gacaacctta atcagcattg cgcataccat accggctatt
     9001 attttattgg ttgtgttgat taccaataat ttctcatcag tgctgtacaa tgcaagaatc
     9061 agcgtagact taacaaatgc ctgtttgcat agatctactc gtacgttatc aataatggcc
     9121 agtcgttcgg cacgctttaa tttattcccg gacatatttt cgattgtttg gattcgagaa
     9181 ttagcttctt tcatgacgac atgtcgggga attattttct gatcatgacg gattaccatt
     9241 gcgtacccac cagatatcgg agttaccagt tcaccagtga caggattctc tacaaaagag
     9301 gaccgtgaaa attctgtttc cccgatttca gaataaggga gttcgagaag atgaccttca
     9361 atagcctgta tactaggtaa tgttgctcgg tacacaattg cgttacgaaa ttttggtaat
     9421 ttcattctat tttcctctgc acaatgtatt agtttctcca caaaacagag aagaacacct
     9481 gcggtggcag ccgcccggat ggattgggtt atgagcccgt cgtccggtga tgctcttctc
     9541 tgttttgtaa aaagagcggt accagccgga agcaagtgta caaactggta ccgccaaagc
     9601 agtggctgtt gtggtgaccg gtgctgatct ccggcttgcg gttatttcag actctcacgg
     9661 gcgtttaatt gccccgccga acagctcttt tccgcaatag ctgcaatgtc tttcgcgcat
     9721 cagcctgcgc attcaccaca acgctaagga ttctctctgg ttgaaaatac ttagctgtta
     9781 tgtgcctgct tttagccaca tcaggcgagg tggacctggt tattccccaa caacaaggat
     9841 ttggttatta tgtaaccctc cccaacaacg ataaggattt atatggatct atgtgtagat
     9901 aagtatgagg agaggcatgt gccgttaaga caaaagcctc cagttcctcc aaaaccaccc
     9961 aaggaaaact gagactaata tgaccagaga cgattataaa ttttatgttc actactctta
    10021 tgtattggag aagatgaatt acacgttgtt aactcgcatt gataaattaa taacgttgat
    10081 gttaatcgtt ctcggttttt cagttttcgc cccgtacagt aatttattta ttttcggttt
    10141 gtttgttgcc gtactttcgg ttcttcagtt ggtctatcag tttggccaag aggctggaat
    10201 gtctaaagag caagcgaggc attacaaaag actgctgatt gagtctgata cccttcctga
    10261 agatcagttg caagctaggt atttaaaaat ccaagatact gatagtaatc catggaaatc
    10321 acttgaacga gcggctttca ttcgggcctg tatttttgct ggtgttccaa ctaagtacga
    10381 tttaacaacc aaagaaaaat ttatagcttg gtgtgcggga gatctacctc tagagaatga
    10441 actaacggat attaatgatg accaacaagc ataatccagt tcacggacat gtacctccga
    10501 agccgaaacc accgaaaccg aagaaatgag acataatagg attggataat gagtcgacca
    10561 atagagcctt ttcatattcc acctcgtccc aggccaccag atccaggccc aaagtgaggg
    10621 ttactgattt ttaaagagcg aagcgtccgg tagggcgctt ttttggtact aacgaatcat
    10681 cctggacttc atatgcccca ggcggctact tcgtgggcgt cctgcctgtt cgttatcttt
    10741 gatataaaat ctaacttaac ttagttttaa tggcaagaga aaacaccaaa cttttcttag
    10801 ttcggtgttt tggttagaga aaaggggggg ctagagttcg tattgaactc ctttgactac
    10861 accaatgatg aggcaattac cattgatcgg gatgttgggg taccggggat ttaatggcac
    10921 taaaaacttt tgagggccat cgataactaa tttttttact gtggcttcgt ttgttccatc
    10981 aagtctagcg atgactattt ttccatgccg gggttctgca tcaggatcta caatcactgt
    11041 tgcgccttct ggtattgttg ggaggccgtt agggttagtc atggagtcgc ctttaacctc
    11101 caatgcaaat gagttatcac caatctttaa tgatgtatct acccacttgt ctacttcact
    11161 aaacacttct gctgtcctgc actcagtaaa ctgcccagcc tgaacccacg agattacagg
    11221 aactctgcgc atgtttgtga cgagtttgcc ttcaaactcc gcaccataaa gaatgtaatc
    11281 tattgacgta ttgaagaact tcgctaattt tgaaagtgct tccccaccag ggacattgat
    11341 gtctttctcc cagtacccca cagcaacgtc acttactcca caaaatttac ccaattcttt
    11401 ctgggacgtt ctggtaactc ttctcagagc ttttatacgc tgaccaaccg tttccatagg
    11461 agcaccattt ctttaattac taagtaatct tagtttttat tgacctaaga tagattgata
    11521 attaacatct aataaaactt agtttggagg gcatatgacg actgacgata tcgaaagcta
    11581 cttcggcagc attgagaaag ttgctgcttt tttcggcata acaactgaag ccgtttatca
    11641 gtggcgaaac cgtcctggcc agttaattcc aaaaggacgt gcagcagagg ctgcatatag
    11701 aacttgcgga cggttgccat ttaaacctga gctttatgaa aaatctaatg gataaatcga
    11761 tttacagaaa ccacagatat gagggcttaa acgtgggtaa agaacctgaa tggaaagttg
    11821 ataagcaacc agcatggctg gtggcagcaa tacgaagaac gattgctgat ttacctcatg
    11881 gctatgagga agcagcagaa attcttggtt tgtataaatc tgatgatatc accccagcaa
    11941 aagatcaatt gcataacaga ctgcgtagcg gtggggatca aatttttcca cttgagtggg
    12001 ccatggtttt acaggatgcc agtggtacca ggcatgtaac ggatgcaata gcccgtcgta
    12061 gtaatggggt gtttgtgccg ctggtggtca ttgatgacat tgacaatggt gacattaatc
    12121 agcggctgat ggagtcaata gaatggattg gcaagcattc ccagtactta cgcaaggcaa
    12181 ctgctgatgg agttattgac caggctgagc gtgagcaaat cgaagagaac agctaccaag
    12241 taatggcgaa gtggcaggag catttaacac tgttatttcg tgttttttgt gcgccggaaa
    12301 agagtaacgc ccgcgagtgt gcagctccgg gcgtcgtggc gtcgattgct tctggttgtg
    12361 gagaaactaa cgcatgaaca gtttaacaac acactaccgt cgctcgcaac tgattgcgct
    12421 tcctgtaccg ggtggaaaag cgaaggtgga gtattgctat gcagtgaatg taccaggtga
    12481 cagggaaatt gtaacccaca gctttgcaga gtgggctgtg ggtgatttca accggcagaa
    12541 ggagacagtc ctttgcgaca agttaaccgc tggttcaaag atcaccacgg agtgcccgtc
    12601 agagtcattc gttgggagcc ggaaacacaa cgggttatct acctccgtga aggctatgaa
    12661 catgaatgct tcagcccact tgagcatttc aggcaaaagt tcagggagat aagggacgat
    12721 catgagcaaa ccagtaatga ttgattctga taattctggg cgttatattg ttctggcagc
    12781 ggcaaaatcc gttgccggga ttggcgtcct gaaattgatt actgagcata accgcgctca
    12841 tgcggttttt tcgtgtcatg agcattgcta cgcccaaatt atggtggggc gtacagggcc
    12901 gacttcggtc gggccgggtt cggtagtctc cggtaacgcc aaccctgtac gtctcaccac
    12961 ctctgtgatt ggcgtcccat gtggtgagtt ttctaaaaaa ctgactaccg gggctgtcac
    13021 tatgactact ctcccaaccc tctctcaacc tgaaattgcc atcgttgatg gtcaggctgt
    13081 tacatcttct ttggctgttg ctgacttctt ctctaaacgt catgacgatg ttctgaaaaa
    13141 gattcgcatt ttggattgtt ctccagagtt ttgtgcccgc aattttgcgg agacatcaat
    13201 tttggtacgc cagcccaacg gcggtactcg caaactacct tgctatcaaa taacccgcga
    13261 tggctttgcg tttcttgcta tgggtttcac gggtaaacgt gctgcccagt tcaaagaggc
    13321 atacatcaat gcctttaacc agatggaaca ggcgctgatc ggaaaggttg atacttcatg
    13381 tgtcgcgcac aatgcccacg ttgtttacct ctacatggct gaaattcacc gcgtttggtt
    13441 agaacaactt tatccgatgc tggtttcagt tcagtctccg ctggcttcat cgctctacga
    13501 ctacatcaac gatggtgtat tcgttgccgg gctggtggac tcggaactga acgaaaagca
    13561 taaggaggtg cgtaagtgag caccaaatta accggctatg tatgggatgg ttgcgcagcg
    13621 tcaggcatga aattatccag tgtggcaatt atggcccgtc tggctgattt cagtaatgac
    13681 gagggtgtgt gctggccatc aattgaaacc attgcccgcc agattggcgc ggggatgagt
    13741 accgtcagaa cggctatcgc acggctggaa gcagaaggct ggttaacgcg taaggcgcgt
    13801 cgccagggta accgcaatgc gtcgaatgtt tatcagctta acgttgcgaa gcttcaggct
    13861 gcggcatttt ctcaactgtc agattctgac ccgtcaaaat ctgacgcatc aaaatctgac
    13921 ccgtcaaaat ttgatgcgtc gaaatctggc aaaaaagcgt gttttcaccc gtcagaatct
    13981 ggcggggatc cgtcagtaaa atcaaaacat gatccgtcag ataaaaaacc ttctcgtccg
    14041 gacgctttgc aaccggacac gcagacggct gaacaggatt ttttaactcg ccatcctgat
    14101 gcggttgtat tcagccctaa aaagcgccag tggggaacgc aggatgattt gacctgcgca
    14161 cagtggctct ggaaaaaaat catcgccctg tacgagcagg ccgccgaatg tgacggcgag
    14221 gtggttcgtc ccaaagaacc gaactggaca gcatgggcaa acgaaattcg cctgatgtgt
    14281 gtgcaggatg gtcgtactca caaacaaatc tgcgagatgt acagccgcgt cagccgtgat
    14341 ccgttctggt gccgtaacgt gctcagcccg tcgaagttgc gggaaaaatg ggatgagctt
    14401 tccctgcgct tatcgccgtc cgtcagcacg cacacagaaa aacgtgaaga cccgtacttc
    14461 aaagccagtt acgacaacgt ggactacagc cagatcccgg aaggattcag ggggtgatca
    14521 tgagtctttt gaatgaagtt cagaaattca ttgaagccca tccgggctgt acttccggag
    14581 acattgcgga tgcttttgca ggttactcac ggcagcgcgt actgcagtca gcaagcaagt
    14641 tacgtcagag tgggcgtgtg gctcaccgtt gtgaagggaa aacacgcaga catttcccac
    14701 accaggctga gatatcgcag gaggagaaac tgcaacctgt tcttgaaacc atacctgtga
    14761 gcaatttcta tgtcggcact aacgacccct gggtgatttt gtgcctgacc cgtcaggctg
    14821 aagaactgga gtccaggggc ttataccgtc gtgctgcaac ggtgtggatg gcggcattcc
    14881 gtgaaagcca ctcccagcca gaacgaaaca attttctggc gcgtcgtgag cagtgcttac
    14941 agaaaagcag caagcgcgca gcatcgggtg aagagtggta tctgtcaggg aattacgtgg
    15001 gggcttaatg agtaataaat attgtcaggc actggtggaa ctgcggaaca aaccagccca
    15061 tgaactgaag gaagtgggcg atcagtggcg cacgccggac aacattttct ggggaattaa
    15121 cactctgttt ggcccgtttg ttctggatct gtttactgac ggtgataacg ccaaatgtgc
    15181 tgcgtattac acggcggaag acaacgcgct ggcgcatgac tggtcagaac gtcttgcgga
    15241 gcttaaaggt gctgcctttg gtaatccccc atacagccgc gccagtcagc atgaggggca
    15301 atacatcacc ggcatgcgtt acatcatgaa acatgccagt gccatgcgtg ataagggcgg
    15361 gcgctatgtt ttcctgatca aagctgccac cagcgaagtg tggtggccgg aagatgcgga
    15421 ccatattgct tttattcgcg ggcgtattgg ttttgaactg cctgcctggt ttatcccgaa
    15481 agacgaaaag caggtgccaa caggtgcttt cttcgctggt gctattgctg ttttcgacaa
    15541 gacctggaag ggagcggcaa tcagctacat cgggcgcgat gaacttgagg catgtggtga
    15601 ggcatttctg gcgcaggttc gccagcaggc ggaaaaactg gtcagggaga tggtggcatg
    15661 aagctaatac tgccttttcc gcccagcgtg aacacctact ggcgacaccc caacaaaggg
    15721 gcatttgctg gtaagagcct gataagcgcg gcggggcgaa aattccagag tgcggcgtgc
    15781 acagcaatag ttgagcagtt acgtcgtctg ccgaaaccaa cgtcggcacc tgcttcagtg
    15841 gagatcgtgt tgtttcctcc ggataaccgg atccgcgatc tggacaacta taacaaggcg
    15901 ctgtttgacg ccctgaccca cgcgggtgtg tgggaagacg acagccaggt gaaaagaatg
    15961 ctggtggagt ggggaccagt tatcccggaa gggaaggtcg agatcactat cagtaagtac
    16021 gagaaaacgg cgggtgcagc cgcctgatta agaggagaaa cgaagtatga ataatctgat
    16081 ggtcattgat ggtattgaag ttcgtcgtga tgcttatggg cgttacagcc tgaacgatct
    16141 gcatcgcgca gcagtagcat ctggtgcaaa tgccagaacc aaggagccag gaaagtttct
    16201 ttccagccaa caaactgttg agcttgttca tgaattgacc aacacccaga atttgggggt
    16261 tgacccggtg agtgtgattc atgggggaaa tgaacgggga acgtatgtct gcaaggaact
    16321 ggtgtatgcc tatgcaatgt ggatcagtcc atcattccat ctgaaggtga tccgtacttt
    16381 tgacatggta accagcgcac cggataaatt atccggacag gctgctgaca agatgcaggc
    16441 tggtgtgatt ctgctggact ttatgcgccg ggaattaaac ctgtctaact cttcagtgct
    16501 tggtgcctgt cagaaactcc aggaggctgt tggcttaccg aatctggcac cgcgctatgc
    16561 cattgatgct cctgccgatg cacccgatgg ctcaagtcgc cccacgctgt cgctaagtgc
    16621 actgctgaaa cagtatggta tccgcctgac ggctaatcag gcatatcacc agatggtgaa
    16681 gctggggatc gtcgagcagc gcgaacgata cagccgtacc gcgattaaca acatcaaaaa
    16741 attctggtcg ctgacggcga aaggctgcat gttcggcaag aacatcacca gtcctgcaaa
    16801 tccgcgcgag acgcagccgc acttcttcga atcccgattc cctgagctgt taaagctgct
    16861 cgataccgtt cattgaggtg accgtgagag cactactgac ccctgaaatt gccccgcgta
    16921 tggggatcgt attgttcaga ccaggttcag agctgatgcc cctgtttatg caggggcgtg
    16981 tcctgctgga gcctgagccg gaacgttatt catctttcgc cagtggtgcc gttccggcgg
    17041 catcacaacc gctggcggat gatcctgccg ttcgggccgt gttccgcaat gaggcagtga
    17101 tccgtcgtgc tggtggcgtg gaatgtcttg aaagctggtt acttcgtgaa aaaggctgcc
    17161 agtggcctca ttccgactgg cacagcgaga acatgaccac aatgcgacac gctccgggtg
    17221 caatccgtct gtgctggcac tgcgataatc agctgcgcga tcagttcacg gaacggctgg
    17281 aatcaatggc aacggataac tgtgcccgct gggtgttgtc tgttgtgcgt cgggatctcg
    17341 gttttgatga cagtcacgtt gtgacaatgc cggaactgtg ctggtggctg attcgtaatg
    17401 atctggcgga tgccttaccg gaaagtgcag cccgtaaggc actgagatta ccgaagcctg
    17461 ttgtgccgac tgttacccgg gaaagtgacc ttgtgccttc ggttcctgcc accagcatca
    17521 tccgggataa ggcgaaaaag gtgctggcgc tgaaagtgga tccggagtcg ccggagtctt
    17581 ttatgttacg cccaaaacgt cgccgctggg ttaatgaaaa gtacacgcgc tgggttaaga
    17641 cacagccgtg tgcatgttgt ggaaagcccg ctgatgatcc ccaccacctg ataggtcacg
    17701 gtcagggtgg aatgggaaca aaagcgcatg acctttttgt gttgcctttg tgcagaaagc
    17761 atcacgacga gctgcatgcg gataccgtgg catttgaaga gaagtatggt tcccaactgg
    17821 agctgatatt tcgttttatc gatcgcgcgc tggcgattgg tgtgctgtcc tgattttgtg
    17881 gagaaagttg atgcgtgata ttcagatggt tcttgaacgc tggggggcat gggcggcaag
    17941 tggtaacacc ggggtggact attctccgat cgctgccgga ttcaaaggac ttttaccatc
    18001 tgccactaaa ccacgtccgg cctgctgcga tgatgacgga cttatcattg aaaactgtct
    18061 tgctcgtctg aagcagaaaa aacctgagga gtattcgctt ctcattgctc attatctgtt
    18121 gcgaatatca aaaagacaga tagccaggac gagaaagaaa agcgaaaaag caatacgaat
    18181 tgagatgcag atagccgaag ggtttattga cggatgtttg tctgtgctgg gggtaagact
    18241 ggagatggac gactggctgc taaaaaagta aaaaatgatt agtgcggtcc gcaaaaagta
    18301 tgtcagtatg ttaagagtgg ttacttcgcc acacagctta aacccgccgc gagcgggttt
    18361 tttatggctg aacgttaaat gcgctggtgg ttgtgaatgc cgactgcggc ggtattttgg
    18421 cgtgacggcg caggtgccgt ataccgatgt ctggacgcat aaaccggtac agttctatcc
    18481 cgggaaacat ccgtgcgaaa aaccggcaga aatgctgcag cagataatca gcgctagcag
    18541 tcgtccgggt gacctgattg cagatttttt catggggtcg ggttcgacag tgaaagcggc
    18601 actggcgctc gggcgtcgtg caattggcgt tgagctggag actgaacgtt ttgagcagac
    18661 ggttcgggaa gtacaggatt tagtcagcca gaacggatga tattgaagaa ttaattacgc
    18721 gtcgttatta tgcggctccc ggccctttag ctcagtggtg agagcgagcg actcataatc
    18781 gccaggtcgc tggttcaaat ccagcaaggg ccaccatcac ataccgccat tagctcatca
    18841 ggatagagca ccagccttcg aagctggttg cgcggggttc gagtcctcga tggcggtcca
    18901 ttatctgtac cctgcgttgt tagctcaacc ggacagagca attgccttct aagcaatcgg
    18961 tcactggttc gaatccagta caacgcgcca gacttatttt tcccggctcg c
//
LOCUS       DAOFSV010000083        18857 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.083, whole genome shotgun sequence.
ACCESSION   DAOFSV010000083 DAOFSV010000000
VERSION     DAOFSV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.083"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            78..611
                     /locus_tag="QBH27_003012"
     CDS             78..611
                     /locus_tag="QBH27_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ7015293.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VFRRDQESALACWGIDR"
     gene            1166..1252
                     /locus_tag="QBH27_003013"
     CDS             1166..1252
                     /locus_tag="QBH27_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015294.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            1474..1659
                     /locus_tag="QBH27_003014"
     CDS             1474..1659
                     /locus_tag="QBH27_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HDQ7015295.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            1656..1748
                     /locus_tag="QBH27_003015"
     CDS             1656..1748
                     /locus_tag="QBH27_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDQ7015296.1"
                     /translation="MMTMQKQLEGAQEYIRTQCTKLAFYYPEDT"
     gene            1745..1960
                     /locus_tag="QBH27_003016"
     CDS             1745..1960
                     /locus_tag="QBH27_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015297.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVLQPVC"
     gene            complement(2159..2359)
                     /locus_tag="QBH27_003017"
     CDS             complement(2159..2359)
                     /locus_tag="QBH27_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDQ7015298.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2401..2766
                     /locus_tag="QBH27_003018"
     CDS             2401..2766
                     /locus_tag="QBH27_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ7015299.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCAECHRQKSAREAASGMPD
                     PTLPEVSGGSGREDDIIGL"
     gene            3057..3620
                     /locus_tag="QBH27_003019"
     CDS             3057..3620
                     /locus_tag="QBH27_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDQ7015300.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNLEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            3617..5278
                     /locus_tag="QBH27_003020"
     CDS             3617..5278
                     /locus_tag="QBH27_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ7015301.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGTNLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            5342..7279
                     /locus_tag="QBH27_003021"
     CDS             5342..7279
                     /locus_tag="QBH27_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDQ7015302.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            7324..7545
                     /locus_tag="QBH27_003022"
     CDS             7324..7545
                     /locus_tag="QBH27_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015303.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            7491..10076
                     /locus_tag="QBH27_003023"
     CDS             7491..10076
                     /locus_tag="QBH27_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ7015304.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMV
                     KEAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR
                     GTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKT
                     FSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRD
                     AGGGK"
     gene            10073..10399
                     /locus_tag="QBH27_003024"
     CDS             10073..10399
                     /locus_tag="QBH27_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDQ7015305.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            10409..10759
                     /locus_tag="QBH27_003025"
     CDS             10409..10759
                     /locus_tag="QBH27_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDQ7015306.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            10756..11202
                     /locus_tag="QBH27_003026"
     CDS             10756..11202
                     /locus_tag="QBH27_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDQ7015307.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            11199..11543
                     /locus_tag="QBH27_003027"
     CDS             11199..11543
                     /locus_tag="QBH27_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDQ7015308.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            11610..12326
                     /locus_tag="QBH27_003028"
     CDS             11610..12326
                     /locus_tag="QBH27_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HDQ7015309.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            12341..12715
                     /locus_tag="QBH27_003029"
     CDS             12341..12715
                     /locus_tag="QBH27_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HDQ7015310.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFAPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            12811..13020
                     /locus_tag="QBH27_003030"
     CDS             12811..13020
                     /locus_tag="QBH27_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDQ7015311.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            13068..16310
                     /locus_tag="QBH27_003031"
     CDS             13068..16310
                     /locus_tag="QBH27_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ7015312.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVARVQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            16303..16644
                     /locus_tag="QBH27_003032"
     CDS             16303..16644
                     /locus_tag="QBH27_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015313.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNLTVRVVCREWSVTDNARHS
                     DFSCTIEQVVN"
     gene            16644..17342
                     /locus_tag="QBH27_003033"
     CDS             16644..17342
                     /locus_tag="QBH27_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDQ7015314.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIDGSGFEMSGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            17353..18096
                     /locus_tag="QBH27_003034"
     CDS             17353..18096
                     /locus_tag="QBH27_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015315.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTSEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            18042..18674
                     /locus_tag="QBH27_003035"
     CDS             18042..18674
                     /locus_tag="QBH27_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDQ7015316.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSLQVPGFRRQMNEGWY
                     QIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVVIGRQG"
BASE COUNT         4218 a         4563 c         6082 g         3994 t
ORIGIN      
        1 ttagcagcct gaggcaacag gctggaactg ataaacatat ccagggctca gaaaccgata
       61 aatcctgata aatatccatg aacgcaaaaa tcagatacgg cctgtcggct gccgttctgg
      121 cgctgattgc cgctggtgcg cctgcgcctg acattctcga ccagtttctg gatgaaaagg
      181 aaggtaacca caccacggca taccgtgatg gcgcgggtat ctggaccatc tgccgcggtg
      241 ccatcctggt ggatggcaaa cctgtcgttc cgggcatgaa gttgtcgaag gaaaaatgcg
      301 accgggttaa cgccattgag cgtgataagg cgctggcatg ggtggagaaa aacatcaaag
      361 tgccattgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac aacattggtc
      421 ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat cgcaggggag
      481 catgcgaagc gattcgctgg tggattaagg acggtggcag agactgccgt attcgctcaa
      541 ataactgtta cggtcaggta ttccgtcgtg accaggagag cgcgctggcg tgctggggaa
      601 tcgacagata agcagaatat tttgctgaaa aatgaggttg gccaacgcgg gcggataaca
      661 cgaagtcctg tgaactggca aaacctaagt gaataaaagt aaaaaccccg tttgttggca
      721 gcaagcgggg ttttgttttt atggcagtaa gctatgggag gctgccttga ttgattttag
      781 caaactgatt agggagttgc gactcatgat tagtcaatta ccaaactgga aatttttgct
      841 ggtctggagc atcccttttt tatgggtagt atcccagtta attgtggcaa ttaaggggta
      901 gctatgtcag acaaactcat aacgccggca aaggtcctgt gtgtgattgt cggtatttca
      961 ttttcactaa tgctggttgc tctttttctg tccctcgcct gggtgatgtt gtcttcgtcg
     1021 gggctgctgg ggtgacagtg actgatgaca tcagcagagc gctggctttt gctattaagt
     1081 gggtggctgt tggtattgct gtgtctccga tgctgtatgg gctggcaaaa ctggtcattg
     1141 cgctgaaatc gtgaacttta aaaagatgag tgctgaactt attcgggcaa tggcatttgc
     1201 cattcgtatt gtggccattg ctgttctggt ctgggcaatc cgttggtggt gatatgaacc
     1261 gtgttctgtg tgtggtgatt attgtcatgc tggtagcctg tggtgtgctt agtctggggc
     1321 tgaatcatta ccgcgataac gccatcacct acaaagcgca gcgcgataaa aaagtcagtg
     1381 agctgaaact ggcgaatgcc actattactg acatgcagca gcgccagcga gatgtcgctg
     1441 cgcttgatgc cagatacacg aaggaattag ccgatgcgag agctgaaaat gaaactctgc
     1501 gcgctgatgt tgccgctggt cgtaagcgcc tgcgggtcaa cgccacctgc cccggtaccg
     1561 tgcgtgaagc caccggcacc tccagcgtgg ataatgcaac cggcccccga ctggcagaca
     1621 ccgctgaacg ggattatttc atcctcagag aacggttgat gacaatgcag aagcagctgg
     1681 aaggggcgca ggaatatatc cgcactcagt gcactaagct ggctttttat tatccggagg
     1741 atacatgaag aaattacggg taaccgtaga accttttcag ggaacaattc cgttccgtat
     1801 tttgcagcgt ggtcgtgttc ttgttgaagg ttcgttcagt ggtaaatgta cgcaattaca
     1861 ctcccggacc tttcaggtga atgccacgaa tgaagagcta accgtggagt gtacgatgaa
     1921 tgccgctaaa tgccgcatgg tattacagcc agtgtgttga gcgaccttat tatccatgcg
     1981 cggtattgtc gccgtattcc tgcattaaca gagaccgcag cccgacaggg agactcctct
     2041 gcgcgagtgt gcggggataa tcaaaaacga tacacaccgg ggtttaccgc gttaacggag
     2101 cgcggcgttg tcccctcata gtcgcctgtc cggtgcgatg gtggaagaaa ccgaacgttc
     2161 atttctcgtt atttgtcatg ctggccgggc gcagatgcgt tgcatctgtt gccagccttc
     2221 tcctgcaggc ttcaataacc cacgctgaaa agttaccgga ccctttatgc tcaagggcga
     2281 tgttgatctg ttcaattatg tgattgggga aacggatatt gcgggttgtg gttctgcggg
     2341 tccggttttt cgatgacata tttatttcct ttactgattg ccatatgacg gggattttac
     2401 atggctgagc ttcgtacact ccagagcaga atcaaaacac tgaatacccg acgggtgaat
     2461 attctgaagg gggagcagcg tcgtgtcagt ggcagtgcac gtgtttccct caagcgtcat
     2521 atctggctga gggacgccgg gcagtgctgt atctgtggtc gtgtggttga cctctgtgac
     2581 agtgaactcg atcaccgaat tgcacttcag ttcggtggtg gtaatgagga gacgaatctt
     2641 tggacgctct gtgccgaatg ccatcgacaa aagtctgctc gtgaagcggc gagtggtatg
     2701 ccggacccga cgctgccgga ggtgtccgga ggtagtggca gagaggacga catcatcgga
     2761 ctgtaaccca acccgggggg ggtatcatcc ggcgtaaaaa acgatcgctt tggacaccgc
     2821 gccccctctc acgcagagaa aaaattcccg tttcagggca gttaacatgt taactggctg
     2881 cccgggcatt tttgcggttt ttatctttat tattcagttt gttgtgcgga aaaaatgtta
     2941 acaggctttt tcagcaaatg ttaaccaggc agcagttaac atttgcggca tgagacgccg
     3001 ggaaaaatgg gctgaaccat acccggctga gtgcgttctg gacccgggag gaggctgtgc
     3061 tgacaacgca aaaacgaaaa tttgcgctgg cgctcatgtc cgggaaaaac aaaacagcgt
     3121 cagccattgc cgccggttat tcggcgaaga ccgccagggt taaaggctcg cagctggcaa
     3181 aagatcctga ggtgcttgcg tttatagccc gtaaacaatg cgagacggtg gaggtggatg
     3241 aggttcctgt ttaccggcag aaaaaatcag agcaggagga taaaccccgt cgccgtgagg
     3301 cggctgcaat accacagccg gacgaaaaca atctggagat gccaccgtcc gcggtgatgt
     3361 ctcctggtat tgaatatatg gaggatggtc ttcccgatcc ggtgaaagcc atggggcgga
     3421 tcctggtgga aaacctctgc attgatccga aactggcact ggatgcggcc tggcgtctgg
     3481 cgcagttcac gcaccataaa aaaggggata ccgggaaaaa atcggcaaaa ggtgatgccg
     3541 cgaaaaaagc ggctaaccgt tttgcggtgc caccgccccc ccgactggtg gtgaataacg
     3601 ataatgaggg caacggatga tacctgtatg gagcacagcc tgcccggact gggcagagcg
     3661 cctgaaaaag gggctgtcga ttattccgga tccgatttat ccggacgagg ccgcacatgc
     3721 cctggcgatt tttaaacaac tgcggattgt ggatgcacct ggtagcccta cgttcgggga
     3781 gtcctgtgca ccgtgggtgt ttgacctggt ggcggccctg tttggctcct acgatgcgca
     3841 gaccggtgta cgccatatca aggaagtttt tatccttatc cccaagaaaa actcgaagtc
     3901 cacgctggcc gcggggatca tgatgactgc actgttactg aactggcggc aggcggcggg
     3961 ttacacgatt ctggccccga ctgtggaggt ggcggccaac gccttcaacc ctgcccggga
     4021 tatggtacga cgtgacgatg atctggatga cctctgccag gtacagaccc atatccggac
     4081 catcacccac cgagtgacgg acaccaccct gaaggtggtg gcagccgatc cgaatacggt
     4141 gtccggtatc aagtccgtgg gtacgctgat tgatgagttg tggctgtttg gcaagcagtg
     4201 caaggcggag gacatgttac gtgaagccat aggcggcctt gcctcccgcc cggaagggtt
     4261 tgtggtgtat acgaccaccc agtcgaatga accgcccgcc ggggtgttca gacagaaact
     4321 gcagtacgcc cgggatgtgc gcgacggcaa aattcatgat ccgcactttc tgccggtgat
     4381 atttgaacac cctcctgaaa tggtggaaag cgggactaac ctgctgatgg aaaacctcgc
     4441 catggtcaat ccgaatctcg gctattcagt ggatgaggcc tttctgtacc gggagtaccg
     4501 taaagcccgg gaagccggtg aagagacatt ccgggggttc atgtcaaaac acgccaatgt
     4561 ggaaattggt cttgccctgc gctctgaccg ctgggcgggg gctgatttct gggaagagca
     4621 gggccgttgt atcagcctgg acgatatcct gcgtcgtgct gatgtggtga cggtggggat
     4681 tgacggcgga gggctggatg atctgctggg gatgtatgtg attgggcgtg accgggagac
     4741 ccgcgaatgg ctgggctggg gccatgcctg ggcgcatgaa accgcggtgg tccgacggaa
     4801 gagcgaggcg tcccggtttc aggatcttgt tgcctgtgga gatatgacca ttgtccggcg
     4861 tgtcggggat gacacggcgg aagtggcgga atatgtgcgt cgcattcatg aggctgagtt
     4921 actggaccat atcggtattg acccgtcagg ggtggggcag attctggatt cactggcgga
     4981 agccgggatc cccgacggaa ttgtggtggg gataagccag ggctggaaac tgggcggggc
     5041 cattaaaacc accgagcgca aactggctga aggggtgctg gtgcatggtg accagcccct
     5101 gatggcctgg tgtgtcggca atgcccgggt ggagcctaaa ggtaacgcca ttcttatcac
     5161 caaacaggcc agtggacggg gaaaaattga cccgctgatg gcgctgttca atgcggtctc
     5221 cctgatgtcc cttaacccgg aaccgaaaaa gaaagaatat gcggtttttt tcatataacc
     5281 ctgttcacac tgtaaccatc acgaaccgct ccggcggttt ttttattttc aggaggctga
     5341 tgtgactctt aaacgggcct gttccctgct gacggtgaaa tccttcagtg aggatgaacg
     5401 ggtgatcacc gggattgcgt caacgccttc tccggatcgg gatggtgaca tcctggagcc
     5461 ggagggcgcg gagtttggca gtgcgatccc gtttctctgg cagcatgacc attcccgccc
     5521 ggtggggcag tgtacggtgc gccgggtcag cgaagggctg gaaatcacgg caacactggt
     5581 gaagcccgta ccggatatgc cgtcgcaact ggctgcccgg ctggatgagg tctgggcggc
     5641 cattaagacc gggctggtca gggggctgtc cgtgggcttc cgtccccatg aatacacctt
     5701 tctggacgga ggcggactgc attttctgcg ctgggaactg atggaggtgt ctgccgtcac
     5761 cgtgcccgcg aatgcggaat gcaccatccg gaccattaaa tcttacgacc gcccgttttc
     5821 tgccgcgtcc ggcaaccgga aaccggtggt gaaaatcgca tcttctgccg gcgctgcggc
     5881 acagtcaaca accgtttttc ataaggaaaa gaccataatg aatattggcg aacagattaa
     5941 aagttttgaa aacaagcgtg cagcgctggc agcctccctt gaggaggtca tgaccaaagc
     6001 cgcagaggaa gggcgcacgc tggatgtgga ggaggaagag cattacgaca acaccgcagc
     6061 ggaaatccgt caggtggatg cgcacctgaa gcgcctgcgt gaactggaag ccggtaaggc
     6121 cgccacggcg cagccggtga aacaggccgg taacgggaat gtggccgcgg tggcttctgc
     6181 gccggtgatc cgtgtggagc agaaactgga taaggggatt ggcttcgccc gctttgccaa
     6241 atcgctggct gcggctaaag gcgtccgatc tgaagccctg gaagtggccc gtcgtcagta
     6301 tccggatgac agtcgtctgc atcatgtcct gaaatcggca gtgggcgcgg ggaccaccac
     6361 ggatccgcag tgggcaggca gcctgtctga atatcaggaa tacgcacagg actttattga
     6421 ttacctgcgt ccgcagacca ttatcgggcg atttggtcag ggcgggatcc ctgcacttcg
     6481 tcaggtgcca ttcaatatcc gtgtgcacgc ccaggtgtcc ggcggtgctg ccggctgggt
     6541 gggtgagggt aaggcaaaac ccctgacgaa gtttgatttt gaatccatca ccttcagtca
     6601 tgcgaaggtg tcggccattg cggtactgac ggaagaattg atccgttttt ccagtccggc
     6661 tgctgatgca ctggtccgta atgcgctggc ggaagcggtg gtggcgcgtc tggatacaga
     6721 ctttgtggac ccgaaaaaag ccgcagtggc agatgtctcc ccggcgtcca tcacccatga
     6781 tgtgaagggc acggcatcaa ccggtaaccc ggatgcggat gcagaggctg cgtttggaca
     6841 gtttgtggca gcaaacctgc agcccaccgg tgcggtctgg ctgatgtcca gcaccaatgc
     6901 cctggcactg tccatgcgta aaaatgcgct gggtcagaag gaatacccgg acatgaccct
     6961 gctgggtggc tccttccagg ggctgccggt gattgtctcc cagtacgtgg gtgaccagct
     7021 ggtgctggtg aatgccccgg atatttatct ggcggatgac ggcggcgtgg cagtggatat
     7081 gtcccgcgag gcatcactgg aaatgcagtc tgagccgacc ggcgacagta ccacgccgtc
     7141 cccggtggag ctggtttcca tgttccagac aggcagcgtg gccatccgtg cggagcgctg
     7201 gatcaactgg cgtcgtcgcc gtactgcggc ggtggcggtg atcaccggag tgaactacgg
     7261 cagtgcgtcc ggcggctgag tctgataagg aggacgggag gcgtgcgcct cccgtaacag
     7321 gttatggcaa agatccgata tctgcagggc acgcatgatg cccgggccgg ggatatccgt
     7381 gatgtggcac agccgtgtgc ggaggtgctg gttcgcctgg gaaaggcgga gtacatcacg
     7441 gtgcgacgtc cggcaggtca gaaaaagaaa cgtgatgcgg agcatggcga atgtggaacc
     7501 ttttacggcg aacccgaaaa aaccagaaat caggacgtga cgtaagagag gcgggctgga
     7561 ccagcctgtt tcaggcggtg gctgagccct tttccggcgc ctggcagcag ggcgtgaaag
     7621 ccgatcctga agccgtcctc tcctttcatg cggtgtttgc atgtatttcg ctgatatccc
     7681 aggatatcgc caaaatgcgg ctgcgtctta tgcagacgga tgcgcatggg atacgcaggg
     7741 aaacgcgccg gggggatatt gcccgcctct gtcgtcgtcc caacgcccag cagaaccgca
     7801 tccagttttt tgaactgtgg ctgaacgcca aactgcgtca tggcaatacg gtggtgctga
     7861 aaatccgtaa tgcccggggg cagatcaaag aactgcgtat tctggactgg agccgggttg
     7921 aacctctggt ggcggatgac ggcgaggtgt tctaccgcat cacgccggac cggaactgcg
     7981 ggatcacgga ggcggtgacg gtgcctgccc gggaagtgat ccacgaccgg tttaactgtt
     8041 tttttcatcc gcttatagga ttgccgccgg tgtatgccgc cgggctggcg gccacgcagg
     8101 ggcatcatat tcaggaaaat tcgacgtctt ttttcagaaa tggcggcagg ccgtccgggg
     8161 tgattgagat ccccggcagt attacggaag aaaatgcgaa aaaactgaag agcaactggg
     8221 acagcgggta tacaggcgaa aatgcgggga aaacggccat tctgagcaac ggggcaaaat
     8281 acaaccccac gacgttttca cctgtggatg cgcagacggt ggaacaactg aagatgaccg
     8341 ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa gattggcgtg ggacaaccgc
     8401 cttccagtga caacgtggag gcgctggagc agcagtatta ttcccagtgc ctgcagacgc
     8461 tgattgagtc cattgaactg ttactggatg aggcgctgga aacgggggaa aacgagagta
     8521 cagaatttga tgtcaccacg ctgctgagaa tggacagtga gcggcgcatg aaaacgctgg
     8581 gggatgcggt gaaaaatacg cttctcacgc ccaatgaggc ccgtaaacgg gagaacctgc
     8641 cgcccctggc cggcggtgat gcactgtatc ttcagcagca gaactacagt ctggaggcgc
     8701 tgtcccgtcg tgatgcccgt gaggatccgt tcgcgtctgc cgggaaaaca gtttcatcac
     8761 agctgcctga cggcgcatct gacggtaata aggcaatcag tgaaacagag catgatgcgg
     8821 tgaaagcgat gttcaggggg gatactgaga aaatgacgga acgggaactg tccattattc
     8881 gtgcactggg agaagaattc tccacagtgc tggcggattt acagcgcaca tttgagggga
     8941 agatggcctc gcaggcacaa gcgtttgaag agaaactgac ttccctgtcg gcggtattac
     9001 agaagcatgt gacggtggat gaggtgcgtc cggttctgca ggcgatggtg gatgacgctg
     9061 tgggggccat tccggtaccg cgtgatggtc gtgattatga tccggatgta ctgcagcagg
     9121 cggtgaatga tgcggtcgca aatattccgc agccggcgga cggtaaaagt ctcaccccgg
     9181 atgatgtgcg tccgatgctt gaacagatgg tgaaggaggc tgtaagccat atccctgttc
     9241 cgcgtgatgg tcgtgactac gatccggaag tactgcagaa ggcggtgaat gatgcggtcg
     9301 caaatattcc gcagccggcg gacggtaaaa gtctcacccc ggatgatgtg cgtccgatgc
     9361 ttgaacagat ggtgaaggag gctgtaagcc atatccctgt tccgcgcgac ggtcgtgact
     9421 atgatcccga tgttctgcag aaggcggtga atgatgcggt cgcaaatatt ccgcagccgg
     9481 cagacggtaa aagtctcacc ccggatgatg tgcgtccgat gcttgaacag atggtgaagg
     9541 aggcggtaag ccatattcat gttccgcgtg atggtcgtga ctacgatccg gatgttctgc
     9601 agaaggcggt tctggatgcg gtgagtgccc tgccggctcc gcaggacggg cgtgatgcca
     9661 cggctctgga aatactcccc gccattgacg atcaaaaatc ctttccccgg ggcacgtatg
     9721 ccacacacca gggcggactc tggcgggcgt atgaaaaaac gcacgggatg cggggatggg
     9781 aatgcctggt tgacggggtg gcggatattg acgtcagcat gacgggtgag cggttgttct
     9841 ctgtggtggt ccggcagagc agtggccagc gtacggaaaa aacattttcc ctgccggtga
     9901 tgctctaccg cggtgtgttc agagccggtg aaacctacca ccccggcgat acggtgacgt
     9961 gggggggctc gctgtggcac tgcaacagta tgaccgaaga taaacccgga gaagctcatt
    10021 catcagcctg gaccctggct gcaaaacgtg ggcgggatgc aggaggcgga aaatgacggc
    10081 attactgaca ctggaagaga tcaaggcaca tctgcgtgtc gaccatgacg cggatgatga
    10141 catgctgatg gacaaggttc gtcaggctac cgccgtgctg ctggcctaca ttcagggcag
    10201 ccgggataaa gtgatccgtg aggacggtga actgatcccg ggcgaggcat taacccggat
    10261 gaagggggct gccatgcgac tgaccgggat gctgtaccgg aatccggatc ttgcggagcg
    10321 ggaagaactg cttcaggggg agctgccgtt ttctgtttcc gtgctgattt acgatttgcg
    10381 ttgtccgacg gtgttatgag gagggggaat ggcaatatct gcaggtcgtc tgacacagat
    10441 gataagtgtt ctgaacccgg tgttaacccg taacgctgcc ggagaaatga cggaagaatg
    10501 ggtgtcatgc gggaaaattc atgcggatat ccgaggcagg agcagccggg agcggatgca
    10561 gtccggtgcg gaaatggcgc aggcggaaat ccgcatctgg gtgcgcggtc agtccggtcg
    10621 ggaaatcacg gcagcgtcac gacttcatgt gctgagtggt ccatggcgtg accggatcct
    10681 gaacgttgtc gggctgcccg tgccggatgc gaccggcggg cgtctggaaa ttctctgtcg
    10741 gctgggaggg gaaaaatgat cgaaaccctg ctggattttt cggggctgga ggacatcagc
    10801 cgcgatttgc agcttctgag tggtgcggaa aataaccggg tgctgcgtga ggcaacccgt
    10861 gcgggtgcga atgtgctgaa agaagaagtg gtgtcacggg caccggtacg caggggaaaa
    10921 ctgcgccgca atgtggtggt cctttcccgg cgctcccgcg atggcgggat ggaatccggt
    10981 gtccatatcc gtggtgttaa tccggacacc ggtaacagcg ataacaccat gaaggcggat
    11041 aacccgcgca atgctttcta ctggcggttt gtggaaatgg ggaccgtgaa tatgccaccg
    11101 cacccgtttg tgcgcccggc gtttgatgtg cgcagtgaac aggcagctca ggtggcgatt
    11161 gcgcggatga accgggccat tgatgaggta ctgagacgat gacggaggcg gatttgtatc
    11221 ctcatctggc gcatcttgcc ggcgggcagg tgtacccgta tgtggtcccc ctgctggatg
    11281 gcaggccgtc ggtggcgctt ccgtgggtgg ttttcagcct gatttcatcg gtgtctgcgg
    11341 acgtgatggg cgggcaggcg gagtcctcag tgtcggtgca gatagacgtt tatgccggga
    11401 ctgtgacgca ggcgcgtcag atacgtcagg acgcccgtga agccataatg ctgctggccc
    11461 cgggatccgt cagtgaaatg caggactata ttccggaaaa ccgctgttac cgtgcaaccc
    11521 tggagtttca ggtcacggtg tgactttttc tttttttcta caaaacccat accccgccgc
    11581 gtgcgggttt tttattatca ggaggcagaa tgtctgcttt gtatgaacgc tcacagctga
    11641 cgcaggtgat gatttcatct gccccggcga ctgctgaaac tatggataag gcggaatatc
    11701 tgcgcctgga ctgcaccatc aaggaagtcc agttcaccgc tggtcagaaa caggatattg
    11761 atgtgaccac gctctgctcc accgagcagg agaacatcaa cggtctgggg gcgtcgtctg
    11821 agatttccat gtcgggtaat ttttatctga atcaggccca gaacgccctg cgtgatgcct
    11881 atgacaatga cgcgttgtat gcgtttaagg tgctgtttcc gtccggtaag ggctttaagt
    11941 tcctggcgga agtgcgtcag cacacctggt catccggtac caacggcgtg gtggcagcaa
    12001 cgttttcact gcgtatgaaa ggcaaaccgg tgtcctttgt ggtaccgctg gcgtttgtga
    12061 aaaatctgga taaaacactt accgtgaata caggtgcgct gctgacaatg tcagtcagtg
    12121 ccaacggggg aacgccgccg tataaatacg cctggaagaa ggatggtcag ccggttgacg
    12181 ggcagacgac agacaccttc agtaagccag gtgcgcagtc cgctgatgcg gggaaatata
    12241 cctgcgtggt gaccgattcg gcagagaaag cacagagtgt gacgtctgtt gaatgcaccg
    12301 tgacagtgag cgcagccgcc ggataagggg atgggtcatc atgaaaaagg atctgaaaac
    12361 gctggcgctg gccagactgt cagggtttcg tcataaaacg gtgaaggtgc cggaatgggg
    12421 taatgtcagc gtggtgctgc gggagccttc ggcagaggcc tggtatctgt ggcaggaagt
    12481 gctcaatggt gatggagagg atgacgatac cctgtcggtg gtggcgaaaa cccgccgtaa
    12541 cctagaagcg gatgtgacgc tgttctgcga tgtcctgtgt gatacggatc tgcagcgggt
    12601 gttcgctccg gacgaccgtg agcaggtgct ggccgtctat ggtccggtac atgcccggtt
    12661 gctgcgtcag gcactggaac tgatcgctga tgcagagtcg gccagaaaaa agtagcccgc
    12721 ccggaaattc gctttctgat gcgacttgcg ctccgtctgg ggcgcacctt atccgaactg
    12781 cggcacagcc tgagtgtgag cgaggcgatg atgtggatgg agttcgacag ggtatccccg
    12841 ctgggtgatg agcgcgggga tatccgtaat gcacagatcg tgaaagcggt ttttggggca
    12901 caggggatga atgttgcact gaaggacgcc atgctctgct ggggcgagga tgaggataag
    12961 ccggaggtgg atccgtttgc ggcgctggaa gacgcgctga gcttcgcagc acagtcatga
    13021 atgatgagaa ccgctgaggc ggttttttta cgcccggaga aaggtgaatg gcgacgttac
    13081 gtgaactgat tatcaaaatt tcggcaaatt cgcagtcatt ccagtcggag atccagcggg
    13141 cttcccgcat gggcagtgaa tattaccgga ccctgcagaa tggcgggcgt caggctgccg
    13201 cagtcgcccg ggtgcagcga cgcgccctgg ctgagctgaa cagccagttg acggaaatcc
    13261 gcgcttcagc tgccggaacg gcgggggcat ttgcaggtgc ctttgccacc ggacacctga
    13321 tttctctggc cgatgaatgg agttccgtga atgcccgact gaaacaggcg tcgcagtcat
    13381 ccgatgaatt ttcgtcatca cagaaagtgc tgatggatat cagccagcgg acgggcacgg
    13441 cattttcaga taatgcggcc ctgtttgccc gctcggcagc ctcaatgcgt gaatatggtt
    13501 acagtgctga tgatgtgctg aaggtgacgg aggccatttc cacggggctg aaaatctccg
    13561 gtgccagtac ggcagaggcg ggctcggtga tcacccagtt cagccaggcg ctggcacagg
    13621 gtgtgttacg cggtgaggaa tttaattcgg tcaatgaaag cggagaccgg atcgtacgtg
    13681 cactggctgc gggtatgggc gtggcccgta aagaccttaa ggcgatggcg gacgacggca
    13741 aactgacggc ggataaagtc gttcctgcgt taatcagcca gctgggggta ttgcgtgatg
    13801 aatatgccgc catgccggaa acggtctctg acgggatcac aaaggtggaa aacgccttta
    13861 tggcctgggt gggtggcgcg aatgaagcca gcggagcgac gaaaacgctc tccggcgtgc
    13921 tgaacggtgt tgccggtaat attgataatg tggcaacagc cgcgggggcg ctggttgccg
    13981 tcggggttgc ccggtacttt ggcaatatgg cctccggagc ggtgtctgcc acggcaggac
    14041 ttgtgacggc agcacgtaat gaagtggcac tggcggaagc acagctcagg gggacgcaga
    14101 ttgccacggc gcgggcaagg gcagccgtgt accgtgcaca gcaggctgtg gcggcagccc
    14161 gcgggacgga gatgcagatt gctgcagaag cccgtctggc ggccacacag gaacgcctga
    14221 acagaaatat tgctgccaga accgcagccc agaatgagct gaacagtaca acggcggtgg
    14281 gctcacgtct gatgactggt gcgttgggac tggttggtgg cgtacccgga ctggtgatgc
    14341 tgggggcagc agcatggtat acgctgtacc agaatcagga gcaggccagg gagtcagcgc
    14401 gccagtatgc actgacgata gatgaaatcg cgcataaaac gccgtcaatg tctttgcctg
    14461 aagcctcaga taatgaagga cgaacacggg cggcgctggc agagcagaac cggctgattg
    14521 atgaacaggc cagccgggtg aaatccctgc aggaaaaaat cgctggatat cagtatgttc
    14581 tggctaaccc tggctggaca accggtgacg gattcatgat aaaccatctg acatcggtga
    14641 agaccgtgac ggaagggctt tctcaggcaa cagagcagct tgccgttgag cagtcccgtc
    14701 tggcacagat gcaggaaaaa gcgcagtcca ttcaggatgt gcttgccggg ctggaagacc
    14761 gtcgtgtggc gttaattcgt cagcaggcgg cagagcagaa taaggtgtac cagtccatgc
    14821 tggttatgaa cggtcagcat acggaattca accgtctgct ggggctgggg aatgaactgc
    14881 ttcagcagcg tcagggactg gtgaatgtac cgttacggct gccgcaggcc accctggatg
    14941 ataaacagca gagcgccctg acaaaaacag aacgtgagct ggccctgtcc cgactgaagg
    15001 gggaggaaaa agagcgtgcc cggctggggt atgcggcgga tgaccttggt tttgtggggg
    15061 attcgtatca ggaggcgaga cagcgttata tcagtaatgc tctggaagcc tggcgtaata
    15121 acgaggcgaa caaacctaaa tcccggggtg gaaaatcaga gacggaaaaa gcggaagaca
    15181 gtttttcccg gctactgaag cagcagaaag agcaactggc actggcgggt cagaatacag
    15241 agctggcgaa gctgaagtac cagactgcgc agggcgaact gaaaaccctg acggagatgc
    15301 agaagcagga actgctgcgt aacgcggccc tgattgacca gcaaaaaatc cgggaacagt
    15361 tgcgatcccg ggaagagacc ctgaagaatg ataatgtggc tgcgcgtgca tcaaatgaag
    15421 ccgaactgct gggatacggg cagggagaac gagccaggga acgcatgcgg gagttgcagc
    15481 agatccgtga cagcttccgc cagaaggatg cggaccttca gtctcagtat cagaccgggg
    15541 atatcagtga ggatttttac agacaggcac gggcacagaa cgcgcagtat ctgagcgaac
    15601 gccttaagga ccaggcagcc ttttatgccg aatcggatgc gcagcgtgcg gactggcaga
    15661 aaggcttgca ggaggggctc agtaactggg tggacaatgc atccgattac gcctcacagg
    15721 cagcacagct tgcgacggag ggtatctcag ggatggtgaa taacatcacg gagatgctga
    15781 acggaaacaa agtggaatgg cgcaactggg cctcatccgt actgcaggaa atctcaaaag
    15841 ttcttatgaa tgctgcgatt gtcaacggaa ttaagacggc ggcaaacggt atgtccggtg
    15901 cgggaggatt tattggcagc attggtgact ggctgggcgg tgcggtggcc aatgcaaaag
    15961 gcggcgtgta tacctcggca aacctgagtg cgtacagcaa cagcattgtg gacacgccca
    16021 cgtactttgc gtttgcaaaa ggggccgggc tgatggggga agccggacct gaagccatta
    16081 tgcccctgac ccgggcggcg gatggctcgc tgggcgtacg cgcggtgggc agtatgaacg
    16141 gcagtgccgg tctggtgtat tccccggtct accacatcgc cattcagaat gacggggcta
    16201 acggacagat aggggcggag gcggcaggca gtcttgtgca gctgattgac cagcgggtgc
    16261 aggcggtgat gctgtccatg cgacgtgacg gaggaatgct gagtggctga gataaaaacg
    16321 ctgcatctgg tcccgcgtga agggatgcag gtgagtgaga aaccgtcggt ggtgagggtt
    16381 cggtttggtg acggttatga acagcgccga ccgacgggac ttaatgccag actgaagacg
    16441 tttcaggcgg tgttccgggt gacggatgaa ccaacccggc gctggctgga tgaattttta
    16501 tcgtggcatg gtggttaccg tgcctttttg tggcgaccgc cgaaacataa cctgacggtg
    16561 agggtggtgt gccgggagtg gagcgtcaca gataacgcca ggcacagtga tttcagttgt
    16621 acgattgagc aggtggtgaa ctgatgcagg atattcgcga agaaagtctg aacgagtcgg
    16681 ttaagtcaga gcagtcaccg cgggtggtac tctgggaaat cgacctgacg gtacagggcg
    16741 gtgagcggta ttttttctgt aatgagctga atgaaaaagg ggagccggtc acctggcagg
    16801 ggcggcaata tcaggcatac ccgattgacg gcagcggttt tgagatgagc gggaagggca
    16861 gcagtgccag accgtcgctg acggtgtcca atctgttcgg tctggtcacc gggatggcgg
    16921 aagacctgca gagtctggtg ggggccacgg tggtccgccg ccgggtgtat gcccgttttc
    16981 tggatgcggt gaatttcgtt gcgggcaatc cggaggcgga cccggagcag gagctgagtg
    17041 accgctgggt ggtggagcag atgtcgcagc tgacagccat gacggcctcg tttgtgctgg
    17101 ctacaccgac cgagacggat ggggcgctgt ttcccggtcg catcatgctg gcgaacacct
    17161 gtatgtggga ttaccgggga gatgaatgcg ggtataacgg tcctgcggtg gcggatgagt
    17221 tcgacaaccc caccacggat atccgtaagg acagatgcag caagtgcatg cgcgggtgtg
    17281 aactgcgcag gaatgtcggc aattttggcg gtttcctttc cattaataaa ctttcgcagt
    17341 aaatcccggt ttatgacaca gactgaatca gcgattctgg cgcatgcccg gcggtgtgcg
    17401 cctgcggagt cgtgcggctt cgtgataagc acgtcggagg gggagtggta tatcccttgt
    17461 gtgaatattt ctgcagagcc ggaggcgtat tttcgtatcg caccggaaga ctggctgcgg
    17521 gcagagatgc agggggagat tgtggcactg gtccacagtc atcccggtgg gctgccctgg
    17581 ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg gctggtctgc
    17641 cggggtgaca ttcacaaatt ccgctgtgtg ccacatctga caggacggcg ctttgagcac
    17701 ggggtgacgg actgttacac gctgttccgg gatgcttatc atctggcggg gactgaaatg
    17761 ccggattttc atcgcgagga tgactggtgg cgcaacggtc agaaccttta cctggacaat
    17821 atggcggtca ccggctttta ccgggtgccc ctgtcctctg cacaggcggg cgatattctg
    17881 ctgtgctgct ttggtgcttc ggtaccgaac catgccgcca tttactgcgg caacggtgag
    17941 ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga aaaatggcaa
    18001 cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt cacggggatt
    18061 tacaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa gccatccggg
    18121 cgctgtcgtt acaggtgccg ggctttcgcc gtcagatgaa cgaaggctgg taccagatac
    18181 gtattgccgg ttatgacacg gcaccggagg cggtgtacgc ccgtcttcac gaacagctgg
    18241 gtgagggaac ggtcatccat attgtgccgc gactggccgg ggccggaaag ggtggactgc
    18301 agattgtgct gggggcggca gccatcgtgg gctctttctt cactgccggg gcatcaatgg
    18361 cgttatgggg ttcagccctg gcagccggtg gtttttctgc caccacgatg ctgttttcac
    18421 ttggagccag catgattctg ggcggtgtgg cccagatgct ggccccgaag gcaaaaacac
    18481 cggattaccg cgcaacggat aacggcagac agaacacgta cttttcctcg ctggataaca
    18541 tgattgccca ggggaacccg atgccggtgc cttacgggga aatgctggtt ggctcccgcc
    18601 gtatatccca ggacatcagc acccgtgatg aaggcggggg cggaacggtc gtggttatcg
    18661 ggcgacaggg ataaaacata aaaaaatccc gcagtgatcg cggagctgcg gggacagaca
    18721 aatgaagatc aatgttaagg agttgttttt gttactcggg caaaaaaaca ctaacgcagc
    18781 gaaattataa gcgccacagt cagtgtgtga aaatgtgaag atattcagaa tttttatgcc
    18841 attaccggtt ttaacca
//
LOCUS       DAOFSV010000084        18318 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.084, whole genome shotgun sequence.
ACCESSION   DAOFSV010000084 DAOFSV010000000
VERSION     DAOFSV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.084"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(369..1214)
                     /gene="sseA"
                     /locus_tag="QBH27_003036"
     CDS             complement(369..1214)
                     /gene="sseA"
                     /locus_tag="QBH27_003036"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDQ7015317.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWLRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            1421..6382
                     /gene="yfhM"
                     /locus_tag="QBH27_003037"
     CDS             1421..6382
                     /gene="yfhM"
                     /locus_tag="QBH27_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDQ7015318.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAALQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGALANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALTGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGGASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            6383..8695
                     /gene="pbpC"
                     /locus_tag="QBH27_003038"
     CDS             6383..8695
                     /gene="pbpC"
                     /locus_tag="QBH27_003038"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDQ7015319.1"
                     /translation="MPRLLTKRGCWIMLTAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFAYGLALDEGVIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            8844..9275
                     /gene="ndk"
                     /locus_tag="QBH27_003039"
     CDS             8844..9275
                     /gene="ndk"
                     /locus_tag="QBH27_003039"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDQ7015320.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            9425..10579
                     /gene="trmG/rlmN"
                     /locus_tag="QBH27_003040"
     CDS             9425..10579
                     /gene="trmG/rlmN"
                     /locus_tag="QBH27_003040"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDQ7015321.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            10864..11871
                     /gene="rodZ"
                     /locus_tag="QBH27_003041"
     CDS             10864..11871
                     /gene="rodZ"
                     /locus_tag="QBH27_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDQ7015322.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTSDCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            11898..13016
                     /gene="ispG"
                     /locus_tag="QBH27_003042"
                     /gene_synonym="gcpE"
     CDS             11898..13016
                     /gene="ispG"
                     /locus_tag="QBH27_003042"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDQ7015323.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            13127..14401
                     /gene="hisS"
                     /locus_tag="QBH27_003043"
     CDS             13127..14401
                     /gene="hisS"
                     /locus_tag="QBH27_003043"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDQ7015324.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            14419..15039
                     /gene="yfgM"
                     /locus_tag="QBH27_003044"
     CDS             14419..15039
                     /gene="yfgM"
                     /locus_tag="QBH27_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDQ7015325.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            15050..16228
                     /gene="bamB"
                     /locus_tag="QBH27_003045"
     CDS             15050..16228
                     /gene="bamB"
                     /locus_tag="QBH27_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDQ7015326.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            16346..17818
                     /gene="der"
                     /locus_tag="QBH27_003046"
     CDS             16346..17818
                     /gene="der"
                     /locus_tag="QBH27_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDQ7015327.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            17881..18096
                     /locus_tag="QBH27_003047"
     CDS             17881..18096
                     /locus_tag="QBH27_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDQ7015328.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVIA"
BASE COUNT         4417 a         4496 c         5109 g         4296 t
ORIGIN      
        1 aataagtacg atacaaaagc accgtatctt cattgtgcgg tccatgcgaa gaccgcatca
       61 cgacagcacc actggcgata ttgccgcgat agcaagcgct gagacgcgct tgccctaacg
      121 gtcgcggaac atcctttcct taagccagcg cccgcttgcg agacggcgga aagggccgca
      181 gtggataata cactaacaga acaataataa ctctctttta cttgacaaaa agagagttac
      241 tggcgagtat tgtgtccgct gagacgcgct tgcattacta cggtaataca acctgctgga
      301 aaggccgcga accagaccag caataaaaaa ccgccaaatt cggcggtttt ttattgcccg
      361 taaaaagctt attttacggg ctcaaccggt aaatctgccc gtgcgcccca ttcactccat
      421 gcgccgtcgt acagtttcac gtttggcaca tccaacgtcg caagtgccaa caaaaccacg
      481 gctgctgtta caccagaacc gcagctgacg ataattggtt tgtcatagct gacaccgcga
      541 ccaaaaaata tcgcatccag ttcatcggtc gtttttagtt cgccttcacg taccagttcc
      601 gtccacggaa cgttcaatgc accgggaata tgtccgcgac gtaaacctgg gcgaggttca
      661 tcaacttctg cgttaaaacg tgtagccggg cgggcatcaa taatttgcgc cgtattttca
      721 tggcttgcca ataatacatc ggttactttc accacggctt caggattaaa cgcggcgtta
      781 aactctcctt ccggcagctc tactgcgcct tcttctaata gcagatcatc acgcagccag
      841 cctgcaagtc caccacccag aatcgacact ttctctacac caaaggtgcg cagcatccac
      901 catgctcgtg gggctgagaa aagattacct tcgtcataga caatcaggtg cttatcctgg
      961 ttaacgccta attcacgcat cgccacggcg aacgtttccg ggcgcggcag catgtgcgga
     1021 agcggggaag tgtgatcaga aagcgcttcg atatcaaaaa acactgcgcc gggaatatgt
     1081 ccattcagat actcctgagc aacgttacga tcttcctgtc caggcgacgc catgcgggca
     1141 tcgataatct gaatttccgg gtcatcaata tgttcggcga gccagtcggc tcctacaaac
     1201 catgtcgtgg acataggcat ctccattttt gccggggtta ggctaaattg tcggatgttt
     1261 agtgaaaagc gacaagcaaa agaggctgat ttggctgtct gagccacaat tctcactaaa
     1321 attcggattg tatgggcgtg atactgaatt acataacgta aattctcacg cataataatt
     1381 attcgacgtt gctaacgggc cattgtggca gggatgaaaa atgaaaaagt tacgcgtagc
     1441 cgcctgcatg ctaatgctgg cgctggcagg gtgcgacaac aacgataacg cgccaacagc
     1501 ggtgaaaaaa gatgcgcctt ctgaagttac taaagcggcc tcttcagaaa acgtgagttc
     1561 agcaaaactt tccgcgccgg agcgacaaaa actggcccaa cagagtgccg gtaaggcgct
     1621 gacattgctg gatctctctg aagttcaact cgatggcgca gccacgctgg tgctgacgtt
     1681 ctccatccct ctcgacccgg atcaggattt ctcacgcgtt attcatgtcg tcgataaaaa
     1741 aagcggcaaa gtggatggtg cctgggagct gtcagataat cttaaagagc tgcgtttacg
     1801 ccacctcgaa ccgaaacgtg atttgatcgt cactattggc aaggaggtca aagcgctcaa
     1861 taacgcaacc tttagtaaag atgacgaaaa aactataact acccgcgata tccaacccag
     1921 cgtcggtttt gccagtcgtg gttcgttgct tcctggtaag gtcgttgaag ggctgccggt
     1981 aatggcgctt aacgttaaca atgtcgatgt taacttcttc cgtgtgaaac cagaatcact
     2041 gcccgctttc attagccagt gggaataccg caattcgctg gcgaactggc agtcagataa
     2101 actgctgcag atggcggatc tggtctacac cggacggttt gatctcaatc ctgcgcgtaa
     2161 cacccgtgaa aaattattgc tgccgctggg cgatatcaaa ccgcttcagc aggcgggcgt
     2221 gtatctggct gtgatgaatc aggctggacg ttacgattac agtaatcccg cgacgctgtt
     2281 tacgttaagt gatattggcg tttcagctca ccgttatcac aatcgtctgg atatctttac
     2341 ccaaagtctg gaaaacggcg cggccctgca aggaattgaa gtctctttat taaatgagaa
     2401 agggcagact ctgactcagg caaccagtga cgctcagggg catgtgcaac tggaaaatga
     2461 taaaaacgcg gcattactgc tggcgcgtaa aaacggtcag acaacgctgc tcgatttaaa
     2521 actcccggcg ctggacttag cggaatttaa cattgctggc gcgccaggct atagcaaaca
     2581 gtttttcatg tttggcccgc gcgatcttta tcgcccgggt gaaacggtca tcctcaatgg
     2641 tttgctgcgt gatgcagacg gtaaagcatt gcccgatcaa cccatcaagt tagacgtgat
     2701 taaacccgac gggcaggtac tcaggagcgt cgttagtcag ccggagaatg gcctctacca
     2761 ctttacctgg ccactcgata gcaatgcggc aaccggtatg tggcatattc gcgctaacac
     2821 gggcgataac cagtatcgga tgtgggattt ccacgtcgaa gattttatgc cagagcgtat
     2881 ggcgctgaat ctgaccggtg agaaaacccc gctaacgccg aaagatgaag tgaaattctc
     2941 cgtggtgggg tactacctgt atggtgcact tgcgaatggt aatactttgc aagggcaact
     3001 tttcctgcgc ccactgcgtg aagctgtgtc agccttacct ggttttgaat tcggcgatat
     3061 agctgccgaa aatctttccc gcacgctgga tgaagttcag ttgacgctgg atgataaagg
     3121 gcgcggcgaa gtttctacag aaagccagtg gaaggaaaca cattctccat tacaggttat
     3181 tttccagggt agtttgctgg aatcgggcgg tcgcccggtg acgcgccgtg ctgagcaggc
     3241 tatctggcct gccgatgcat tgccggggat ccgtccgcag ttcgcctcga aatcggttta
     3301 cgattatcgc accgacagca cggtgaaaca gcccattgtt gatgaaggca gtaacgccgc
     3361 ttttgacatc gtttatagcg atgcgcaagg cgtgaaaaaa gccgtgtcgg gcttgcaggt
     3421 gcgcctgatt cgcgaacgcc gcgattacta ctggaactgg tcggaatatg aaggctggca
     3481 gtcacagttt gatcaaaaag atctgatcga aaatgaacaa actctggatc tgaaagcgga
     3541 cgaaaccggt aaggtcagtt tcccggtaga gtggggcgct tatcgtctgg aagttaaagc
     3601 gccgaatgaa gcggtcagta gtgtgcgttt ctgggctggt tatagctggc aggataacag
     3661 cgacgggggt ggtgcagtgc gacccgaccg tgtcacgctg aaactggata aagccagtta
     3721 tcgccctggc gataccatta agttgcatat tgccgcgcca acggcgggta aaggttatgc
     3781 gatggtcgag tccagtgaag ggccgttgtg gtggcaagag attgatgttc cggctcaagg
     3841 gctggatctg acgattccgg tcgataaaac ctggaatcgt catgatctgt atttaagtac
     3901 gctggtggta cgtcctggcg ataaatctcg ctccgcgacg ccaaaacgcg cggttggggt
     3961 gttgcatctg ccgcttggtg atgaaaaccg tcgcctcgat ctggcgctgg aaacccccac
     4021 caaaatgcgc cccaatcagc cattaaccgt gaaaattaaa gccagcaata aaaatggtga
     4081 gatgccaaaa caggtgaatg tgctggtgtc tgccgttgat agcggtgtgc tgaatattac
     4141 tgattacgtc acgccagatc cgtggcaggc gttctttggt cagaaacgct atggcgcaga
     4201 catttacgat atttacggtc aggttattga aggtcagggg cgtctggcag ctctgcgttt
     4261 tggtggtgat ggtgatgagc tgaaacgtgg tggtaaaccg ccggtcaatc acgtcaatat
     4321 tgtcgcgcag caggcgctgc cggtaacgct caatgaacag ggcgaaggct cggttacact
     4381 gccaattggc gattttaacg gtgaattgcg cgtcatggcg caagcctgga cggcagatga
     4441 cttcggaagc aacgaaagca aagtgattgt tgccgcaccg gtgattgctg aacttaacat
     4501 gccgcgcttt atggcgagtg gcgatacctc gcgcctgacg ctggatatca ctaatcttac
     4561 cgataaaccg caaaaactta atgttgctct gaccgccagt ggcttgcttg aactggtgag
     4621 taattccccc gcacctgttg aattagcgcc aggggtgcgt actacgctgt ttatcccggt
     4681 gcgtgctctg acgggttatg gcgatggtga cattcaggcc accattagcg ggttagcgtt
     4741 accgggggaa accgttgccg atcagcataa gcagtggaaa atcggcgtcc gtccggcatt
     4801 cccggcacaa acggtcaatt acggtacggc gttacagcct ggtgaaacct gggcgctccc
     4861 ggctgatgga ttgcaaaact tctcgcctgt tacgctggaa gggcagttat tgttgagcgg
     4921 caaaccaccg ctgaacatcg cacgttatat caaagagtta aaagcgtatc cgtacggctg
     4981 tctcgagcaa accgccagcg gcctgttccc gtcactttat accaatgcag cccaactgca
     5041 ggcgttgggc atcaaaggcg acagtgatga gaaacgccgt gcatcggtcg atatcggcat
     5101 ttcccgtttg ctgcaaatgc aacgtgataa cggcggcttt gcgctgtggg ataaaaacgg
     5161 tgacgaagag tactggctga cggcttacgt gatggatttc ctggtccgcg caggcgaaca
     5221 gggttacagc gtaccgacag acgccattaa ccggggtaat gagcgtctgc tgcgctattt
     5281 acaagatccg ggcatgatat cgatcccgta cgctgataat ctcaaagcca gtaaattcgc
     5341 cgtacagtct tacgctgcgc tggtgttggc ccgtcagcaa aaagcaccac tgggtgcgct
     5401 gcgtgaaatc tgggagcatc gtgcagatgc cgcttctggt ttaccgctgc tgcaacttgg
     5461 cgttgcgctg aaaatcatgg gtgatgcaac gcgtggtgaa gaagcgattg tgctggcgct
     5521 gaaaacgccg cgtaatagtg atgagcggat atggctgggt gattacggta gtccactgcg
     5581 cgacagcgcg ttgatgctct cattgctgga agagaacaaa ctgctacccg atgagcagta
     5641 ctccctgttg aacacacttt cgcagcaggc gttcggtgaa cgctggctat cgacgcagga
     5701 aagtaacgcg ttgttcctgg ctgcccgtac tcttcaggat ttaccgggca aatggcaggc
     5761 gcaaaccact ttctctgctg agccgctgac aggcgagaaa gcgcaaacca gcaatctgaa
     5821 tagcgatcaa cttgccacct tgcaggtgac caacagtggc gatcagccgt tatggctgcg
     5881 tgtggatgcc agcggttatc cgcaatccgc acctttaccg gcgagcaatg tgctgcaaat
     5941 tgagcgtcat attctgggta ctgacggtaa gagcaaatcg ctggactcgt tacgtagcgg
     6001 cgatctggtg ctggtgtggt tgcaggttaa agccagtaac agtgtaccgg atgcgttagt
     6061 cgtggatctg ttgcctgcgg ggctggaact ggaaaaccag aatctggcga acggcggcgc
     6121 cagcctggag caaagtggtg gtgaagtgca gaacttgctg aaccagatgc agcaggcgag
     6181 cattaagcac atagagttcc gtgacgatcg ctttgtggcg gcggttgccg ttgatgaata
     6241 tcaaccggta acgctggtgt atctggcgcg ggcggtgacg ccgggaacgt atcaggtgcc
     6301 gcagcctatg gtggaatcaa tgtatgttcc tcaatggcgg gcgactggtg cggctgacga
     6361 tctgttgata gtcagaccgt aaatgcctcg cctgttaacc aaacgcggct gctggataat
     6421 gctgacagcc gcgcctttta tcatcattct ggcagcatgg gcagcagata aactctggcc
     6481 tctgccattg caagaagtga atcctgcacg cgtggtggtg gcgcaggatg gtacgccact
     6541 ctggcgtttc gccgatgctg acggcatctg gcgttatccg gtaacaatcg aagatgtttc
     6601 tccacgttac cttgaagcac tgatcaatta tgaagatcgc tggttctgga agcatccggg
     6661 ggtgaatccg ttctcggtgg cgcgtgcagc ctggcaagat ctcacgtcgg gtcgggtcat
     6721 ttccggcggc agcacgctca ccatgcaggt ggcgcgcctg cttgatcctc accccaaaac
     6781 gtttggcggc aaaattcgtc agctctggcg cgcgttgcaa ctggaatggc atctgtctaa
     6841 acgtgaaatt ctgaccttgt atcttaaccg tgccccgttt ggcggtacgt tgcaggggat
     6901 tggtgcggca agttgggctt atctcgggaa atcgcctgcg aatttaagct attccgaggc
     6961 ggcaatgctg gcggtgttgc cgcaagcgcc cagccgtctt cgcccggatc gttggccgga
     7021 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gccgtgcaag gtgtgtggtc
     7081 ccgtgagcgg gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
     7141 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
     7201 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
     7261 acgattgcca ccgcgcagct cactggcgat gatcgtggtt gatcataccg atatgcgtgt
     7321 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
     7381 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgcttatg gtctggcgct
     7441 ggatgaaggc gtgatccacc cggcatcgct gttgcaagac gtcccccgac gcaccggtga
     7501 ttaccgacct ggcaactttg acagcggttt tcatggcccg atcagcatga gtgaggcgct
     7561 ggtgcgatcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     7621 tgcggcaaag ttacgcaatg ttggattgcc gttgtatctg cccaacggtg ctgcgccgaa
     7681 tctttcgctc attctcggcg gcgctggcgc aaaactggaa gatatggcag cagcgtatac
     7741 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     7801 tgaacggcct ttaatgtcgt cgggggctgc gtggatcatt cgccggatta tggctgatga
     7861 agcgcaaccc ttgccggatg gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     7921 caccagctat ggctatcgtg atgcctgggc gattggggtt aacgctcgct atgtcattgg
     7981 gatctggact ggcagaccgg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     8041 cgtaccgctg ttgaatcagg ttaataatat cttactgtcg cgcagtgtga atttgccgga
     8101 agacccacgt cctgactctg taagtcgtgg cgttatctgc tggccgggcg gacagtcttt
     8161 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg acgggagcca
     8221 gccgccaact ctattactgc cggagcagga aggcattaat ggcattcgtt tccccatctg
     8281 gctggatgaa aacggcaaac gtgttgccgc tgattgccct caggcgcgac aagaaatgat
     8341 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     8401 cttgcctccg gcctcgacaa tttgcccgcc atacggtcac gatgcacaac tcccgctgca
     8461 gctaacaggt gtgcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     8521 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga acggcgaacc
     8581 gttaactgaa cgtgggcgca acgtgaccct gcatttgacg gataaaggcg attatcaatt
     8641 gctggtgatg gatgacgtgg ggcaaatagc gacagtgaaa tttgtcatgc aatagtctgt
     8701 tttgtccctg attgttgctt aaactgggtt tattttaaaa aaatgttacc ttcatcaata
     8761 gtcaacggcc ctgttgctca ttataatccg cgccatctcg tacgctggta cagacaacaa
     8821 cagaacaatt tacagaggta aaaatggcta ttgaacgtac tttttccatc atcaaaccga
     8881 acgcagtagc aaaaaacgtg attggtagta tctttgcgcg ctttgaagct gcagggttca
     8941 aaattgtcgg caccaaaatg ctgcacctga ccgttgaaca ggctcgtggc ttttatgctg
     9001 aacacgatgg aaaaccgttc tttgatggtc tggttgaatt catgacttct ggcccgatcg
     9061 ttgtttccgt gctggaaggt gaaaatgctg ttcagcgtca ccgcgatctg ctgggcgcga
     9121 ccaatccggc aaacgcactg gccggtactc tgcgtgctga ttacgctgac agcctgaccg
     9181 aaaatggtac ccacggttct gattccgtcg aatctgccgc tcgcgaaatc gcttatttct
     9241 tcggcgaagg cgaagtgtgc ccgcgcaccc gttaataatt tcgtaaatgc cgcgtgcaaa
     9301 cgtggcatcc gtgcgccaga atttgtacaa tgcagcgccc ccggacgagc agccgctcac
     9361 cggggcgttt cttttttcaa ccctccaggg gccataacgt gtaataacga ggccggaata
     9421 gcatatgtct gaacaattag tcacacctga aaacgtcacc acgaaagatg gaaaaatcaa
     9481 cctgctggat ctcaaccgtc agcagatgcg ggagtttttt aaagatttag gtgaaaaacc
     9541 cttccgcgcc gatcaggtga tgaagtggat gtatcactat tgctgcgaca actttgatga
     9601 gatgaccgac atcaataaag tgttgcgcgg caaactgaaa gaggtggcgg aaatccgtgc
     9661 accggaagtg gttgaagaac agcgttcatc tgacggcacc attaaatggg cgatcgccgt
     9721 tggcgatcag cgcgttgaaa cggtgtatat cccggaagac gaccgtgcca cgctctgcgt
     9781 ctcttcgcag gtggggtgtg cgctggagtg taaattctgt tccaccgccc agcagggctt
     9841 taatcgcaac ctgcgggtat cggaaattat cggccaggtg tggcgtgcgg cgaaaattgt
     9901 cggcgcagca aaagtcaccg gtcagcgccc aatcactaac gtagtgatga tgggtatggg
     9961 cgagccgctg ctcaacctga acaacgtcgt tccggcgatg gaaatcatgc ttgatgattt
    10021 cggttttggc ctgtcaaaac gtcgcgtcac gctttccact tccggtgtgg ttccggcgct
    10081 ggataaactc ggcgatatga tcgacgttgc gctggcaatt tccctgcacg cgccgaacga
    10141 cgaaattcgt gacgaaattg tgccgatcaa caaaaagtac aatatcgaaa cgttccttgc
    10201 ggcggtgcgc cgttatctgg agaaatccaa cgccaatcag ggccgagtca ctattgaata
    10261 cgtgatgctt gaccacgtta acgacggcac tgaacacgcg caccaactgg cagaactgct
    10321 gaaagatacg ccgtgtaaga tcaacctgat cccgtggaac ccgttcccgg gcgcgccgta
    10381 tggacgcagt tcgaacagcc gtatcgaccg tttctcaaaa gtgctgatga gctacggttt
    10441 caccactatt gtgcgtaaaa ctcgtggtga tgacattgat gctgcctgtg gtcagttggc
    10501 gggcgatgtt atcgaccgta cgaaacgtac cctgcgtaaa cggatgcagg gtgaagccat
    10561 cgacattaaa gcggtctgat agccattacg ccacggttac tttatgtacc gtggcgtaat
    10621 cttttatgaa tgaagtatta agttgcaatt gtctggtcaa tattaacggt gcgtttttgc
    10681 tgactttaag gcagtatgta acgatgcaac agtaagttag ccttagcgaa tgtgggcttt
    10741 gtcacgagca cacagacggt cttatactgt atgataacgg ttaacttaac ggatgtttcg
    10801 cggtgtgggt gagcatgatg gttcaccggc atctcaattc tcatttaaac gtacctgcag
    10861 cgaatgaata ctgaagccac gcacgaccaa aatgaagcac ttactaccgg cgctcgcctg
    10921 cgtaatgctc gcgaacaact aggacttagt caacaggccg ttgccgagcg actttgcctg
    10981 aaggtttcca cggtacgcga cattgaagaa gataaggcac ccgccgatct tgcttcaaca
    11041 ttcctgcgcg gatatatccg ctcttatgcg cgtctggtac atattccaga agaagaactg
    11101 ctgccagggc tggaaaagca ggctccacta cgggctgcaa aagttgcgcc gatgcagagt
    11161 ttttccctcg gtaaacgccg caaaaaacgt gacggctggc tgatgacctt cacctggctg
    11221 gtgttgtttg tggttattgg tctgagcggt gcctggtggt ggcaagacca caaagctcag
    11281 caggaagaga tcaccactat ggccgatcaa tcttctgcgg aactgaataa taatcaaagc
    11341 cagagtgtac cgttagatac gtcgacaacc acagatcagg ctatggcgac cacgccgaca
    11401 tcacctgtgg ataccaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca
    11461 gctgttgatc cgcaacagaa tgcggttgtt ccaccttcgc aggcaaatgt tgataccgct
    11521 gcgactccgg caccagcggc aacaacaaca ccagatggcg ctgcgccgtt gccaaccgat
    11581 caggctggcg tgaccacgcc ggcggttgac ccgaatgcgc tggtgatgaa ctttacttcc
    11641 gattgctggc tggaagtcac cgatgctacc ggtaaaaaac tgtttagcgg tatgcagcgt
    11701 aaagacggta atttgaactt aaccggccag gcaccgtaca aactgaaaat tggtgcgcca
    11761 gccgcagtac agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact
    11821 aaccaggttg cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt
    11881 aacgcgggag atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta
    11941 tttacgttgg gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca
    12001 atacgcgtac gacagacgtc gaagcaacgg tcaatcaaat caaagcgctg gaacgcgttg
    12061 gcgctgatat cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca
    12121 tcaaacagca ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc
    12181 tgaaagtagc ggaatacggc gtcgattgtc tgcgtattaa ccctggcaat atcggtaatg
    12241 aagagcgtat tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg
    12301 gcgttaacgc cggatcgttg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc
    12361 aggcgttgct ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc
    12421 agttcaaagt cagtgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc
    12481 tggcaaaaca aatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcgcgca
    12541 gcggggcagt aaaatccgct attggtttag gtctgctgct gtctgaaggc atcggcgaca
    12601 cgctgcgcgt atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt
    12661 tgaaatcgct gcgtatccgt tcgcgcggga tcaacttcat cgcctgcccg acctgttcgc
    12721 gtcaggaatt tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatataa
    12781 tcactccgat ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg
    12841 tttctacact cggcgtcact ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc
    12901 gcaaagaccg tctggacaac aacgatatga tcgatcagct ggaagcgcgc attcgtgcga
    12961 aagccagtca gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgaa aaataataac
    13021 gtgatgggaa gcgcctcgct tcccgtgtat gattgaaccc gcatggctcc cgaaacattg
    13081 agggaagcgt tgagggttca tttttatatt cagaaagaga ataaacgtgg caaaaaacat
    13141 tcaagccatt cgcggcatga acgattacct gcctggcgaa acggccatct ggcagcgcat
    13201 tgaaggcaca ctgaaaaacg tgctcggcag ctacggttac agtgaaatcc gcttgccgat
    13261 tgtagagcag accccgctat tcaaacgtgc gattggtgaa gtcaccgacg tggttgaaaa
    13321 agagatgtac acctttgagg atcgcaatgg cgacagcctg actctgcgcc ctgaagggac
    13381 ggcgggctgt gtacgcgccg gcatcgagca tggtcttctg tacaatcagg aacagcgtct
    13441 gtggtatatc gggcctatgt tccgtcacga gcgtccgcag aaagggcgtt atcgtcagtt
    13501 ccatcagttg ggctgcgaag ttttcggtct gcaaggtccg gatatcgacg ccgaactgat
    13561 tatgctcact gcccgctggt ggcgcgcgct gggtatttcc gagcacgtaa ctcttgagct
    13621 gaactctatc ggttcgctgg aagctcgtgc gaattaccgc gatgcgctgg tggcattcct
    13681 tgagcagcat aaagaaaagc tggacgaaga ctgcaaacgc cgcatgtaca ctaacccgtt
    13741 gcgcgtactg gattcaaaaa atccggaagt gcaggtgctt ctcaacgacg ctccggcatt
    13801 aggcgattat ctggacgagg aatctcgtga gcactttgcc ggtctgtgca aactgcttga
    13861 gagcgcgggg atcgcttaca ctgtaaacca gcgtctggtg cgcggtctgg attactataa
    13921 ccgtaccgtt ttcgagtggg tgactaacag cctcggttcc cagggcaccg tgtgtgcagg
    13981 tggtcgttat gacggtctgg tggaacaact gggtggtcgt gcaacaccgg cagtcggttt
    14041 tgcgatgggc ctcgaacgtc ttgtattgtt agttcaggcc gttaatccgg aatttaaagc
    14101 cgatcctgtt gtcgatatat acctggtggc ttcaggtgct gacacacaat ctgcggctat
    14161 ggcattagca gagcgtctgc gtgatgaatt accgggtgtg aaactgatga ccaaccacgg
    14221 cggcggcaac tttaagaaac agtttgcccg tgctgataaa tggggtgccc gcgttgctgt
    14281 ggtgctgggt gagtctgaag tggctaacgg cacagcagta gtgaaggatt tgcgctctgg
    14341 tgagcaaact gcagttgcgc aggatagcgt agccgcgcat ttgcgcacgt tactgggtta
    14401 aggaaggaga aggacagcgt ggaaatttac gagaacgaaa acgaccaggt tgaagcggtt
    14461 aaacgctttt ttgctgaaaa tggcaaagca ctggctgttg gggtgatttt gggcgttggc
    14521 gcactgattg gctggcgcta ctggaatagc catcaggttg attctgcacg ctccgcttct
    14581 cttgcctatc aaaatgcggt taccgcagtg agcgaaggca aaccggatag catcccagcg
    14641 gcggaaaaat ttgctgctga aaataaaaat acttatggtg cgctggcttc tttggaactt
    14701 gcgcagcaat ttgttgacaa aaatgaacta gagaaagctg ccgcccagtt acaacagggg
    14761 ctggcagaca cgagcgatga aaatctcaaa gccgtgataa atctgcgtct tgctcgcgtt
    14821 caggtacagc tcaagcaggc tgatgccgcg ctaaaaaccc ttgatgctat caaaggtgaa
    14881 gggtgggctg ccattgttgc cgacctgcgt ggtgaagcat tgctgagcaa aggtgataag
    14941 caaggtgcgc gtagtgcatg ggaagcaggc gtgaaaagcg atgttacccc ggcactgagc
    15001 gaaatgatgc agatgaaaat taataatttg tccatctgag agggacccga tgcaattgcg
    15061 taaattactg ctgccaggac tgctttccgt taccctttta agcggctgtt cgctgtttaa
    15121 cagcgaagaa gatgtggtaa agatgtcccc attgccaacc gttgaaaacc agtttacgcc
    15181 gactactgtg tggagcactt ccgttggtag cggcattggc aacttctatt ccaatcttca
    15241 tccggcactg gcggacaacg ttgtctatgc agcggaccgc gctggtttag taaaagcgct
    15301 gaatgcggat gacggcaaag aaatctggtc tgtcaacctg gccgagaaag atggctggtt
    15361 ctctaaagat cctgcattac tttctggcgg cgtgaccgtg tctggtgggc atgtctacat
    15421 tggcaccgaa aaggcgcagg tttacgcgct gaataccagc gatggtactg tggcatggca
    15481 aactaaagtc gcgggtgaag cactttcgcg cccggtggtc agcgacggtc tggtgttaat
    15541 ccacaccagt aacggtcagt tacaagcgct gaacgaagct gacggcgctg tcaaatggac
    15601 agttaacctc gatatgcctt cgctctcttt gcgtggcgag tctgcgccgg caacggcttt
    15661 tggtgcggcc gtcgtgggtg gcgataatgg tcgcgtcagc gcagtgctga tggaacaggg
    15721 ccagatgatt tggcagcagc gtatttccca ggcgaccggt tctaccgaaa ttgaccgtct
    15781 gagcgatgtt gacacgactc ccgtcgttgt taacggcgtt gttttcgcgc tggcctataa
    15841 tggtaacctg acggcgcttg atctgcgcag tggtcagatt atgtggaaac gcgaactggg
    15901 ttcggtgaat gatttcatcg tcgacggcaa tcgcatctat ctggtcgatc aaaatgaccg
    15961 ggtgatggcg ttgaccattg atggcggcgt tacgctgtgg acacaaagcg atctgctgca
    16021 tcgcctgctg acttctccgg tgctgtataa tggcaacctg gtggtaggcg acagtgaagg
    16081 ttatctgcac tggattaacg tcgaagatgg tcgtttcgtt gcccagcaaa aagttgatag
    16141 ttccggtttc cagactgaac cggttgctgc tgacggcaaa ctgctgatcc aggcaaaaga
    16201 cggaaccgtg tactctatta cacgttaatc gtctctgtcg ttcactttga aaacggctcc
    16261 tggacagggg ccgttttcct gtttttaaca acgacacgaa tatagcgtgc gttgtctgat
    16321 gatttataaa aatgaggctt taaacatggt acctgtggtc gcgcttgtcg ggcgccctaa
    16381 cgtaggaaaa tccacgttat ttaaccgtct aactcgcacc cgagatgcgc tggttgcgga
    16441 tttcccgggt ctgactcgtg accgtaagta cggtcgtgcg gaaattgaag gccgtgagtt
    16501 tatctgcatt gataccggcg ggattgatgg cacagaagac ggtgtagaaa cccgcatggc
    16561 ggaacagtcg ctgctggcga ttgaagaagc ggacgtcgta ctgtttatgg tggatgcgcg
    16621 cgcgggcctg atgccggcgg atgaagcgat tgctaaacat ctgcgctccc gtgaaaaacc
    16681 gaccttcctg gtggcaaaca aaactgacgg tctggatccc gatcaggcag tggttgattt
    16741 ctacgcgctt ggtttaggtg aaatctaccc gatcgccgcg tctcacggtc gtggcgtatt
    16801 aagtctgctg gagcacgtgc tgctgccgtg gatggaagat ctcgcaccgc aagaagaagt
    16861 cgacgaagac gccgaatact gggcgcaatt tgaagcggaa gagaacggcg aagaagaaga
    16921 ggaagacgac ttcgacccgc aaagtctgcc gatcaaactg gcgattgtgg gtcgcccgaa
    16981 cgtaggtaag tctacactca ctaaccgtat tcttggtgaa gagcgcgttg ttgtttacga
    17041 catgcctggc acgacgcgtg acagtatcta cattccaatg gaacgcgacg gacgtgagta
    17101 tgtgctcatt gacaccgctg gcgtacgtaa acgcggcaaa atcaccgatg cggtagagaa
    17161 attctcggta atcaaaacgt tgcaggccat tgaagatgcc aacgtggtga tgttggtgat
    17221 tgatgcgcgc gaaggtattt ccgatcagga tctctccttg ctgggcttta ttctcaatag
    17281 tgggcgctca cttgtgattg tagtgaacaa gtgggacggc ctgagccagg aagtgaaaga
    17341 gcaggtgaaa gagacgctgg acttccgtct gggctttatc gattttgctc gtgtgcactt
    17401 tatctctgcc ttgcacggca gtggtgttgg aaacttgttt gaatcagtac gtgaagcgta
    17461 tgacagctcc acccgtcgtg tggggacctc tatgctgacg cgcatcatga cgatggctgt
    17521 tgaagatcac caaccgccgc tggtacgcgg tcgtcgtgtg aagctgaaat acgcccacgc
    17581 cgggggttat aacccgccga ttgtggtgat tcacggcaat caggtgaaag acctgcctga
    17641 ttcctacaag cgttacctga tgaactactt ccgcaaatcc ctggacgtaa tgggatcgcc
    17701 gattcgtatt cagttcaaag aaggggaaaa cccgtatgcg aacaagcgta ataccctgac
    17761 gccgactcag atgcgcaagc gtaagcgtct gatgaagcac atcaagaaaa gtaagtaatc
    17821 acttatctgg tttccctttc cacacggaga gggaaaccga ataacacgcg aggagtattt
    17881 atggcactca actgtccaac ctgcaaaacc cctctcgaac gcaatggcga gactgctcac
    17941 tgtgcaactt gcgacaaaga ctttaccgtc caggcacttt gccctgagtg tcatcagccg
    18001 ttacaagtgt taaaagcttg tggtgctgtg gattatttct gccaacatgg gcacggttta
    18061 atatctaaaa aacgcgttga atttgttatt gcttaatatc acttgaatgt attgttttgt
    18121 catttgtatt taatgtgcaa atgataaaac aatctgttat ggtggaataa ctaatgaaag
    18181 cgagcgaaat cactaattta aataattaag aatatattta ttctttataa catgtctttc
    18241 tgaaaaatca ggaatataat attaaaaatt atttttgact ttatcgtcac agggaaagca
    18301 catgctgcga tggaaacg
//
LOCUS       DAOFSV010000085        18274 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.085, whole genome shotgun sequence.
ACCESSION   DAOFSV010000085 DAOFSV010000000
VERSION     DAOFSV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.085"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            402..968
                     /locus_tag="QBH27_003048"
     CDS             402..968
                     /locus_tag="QBH27_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015329.1"
                     /translation="MASQAEVAAHLLLSDRRLRDLAKLPGAPVPQGRGDWELDAWRHF
                     YIRYLRSNRRDTTSNDEPEAGDNSPEKNREQWLKNEERQERILMARVKRRILAKRYAP
                     IELISVAVSRVAVELRTRVESWPPRLKKVWPEMPQEASSVLREELAIALNELSDIRID
                     FSDYDVSDIERDLDRVESLARDDTDDGC"
     gene            955..2817
                     /locus_tag="QBH27_003049"
     CDS             955..2817
                     /locus_tag="QBH27_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDQ7015330.1"
                     /translation="MTGVEWADKYFYLPEGSSHIAGHWTTQPVQVVMLNMMTNDAIKI
                     VSVRKSARLGYTKILVAALLYFAEHKKRSAVVYQPIDDESDGFVADEVDPAIAEMPVI
                     QKIFPDWDKSNERNNLQRKEMSGAILDFRGASAPGNFRRLTKQVVEGDEVDGWPLEVA
                     KKGKGEGSPIELALVRIKGAAYPKAIFGSTPTVTGKSHIEMLEDAADLTFRFYLTCPH
                     CGEEQVLVFGFDGIEYGLKWDNSLQTNEAKSSSAYYQCCHCPEHFYYRDLEKMEFGGR
                     WIAEDCTWTRDGIHFFDHDGGVVRAPKHAAIVINALYSLNLDGWGEIVSEWLKAKGDP
                     LKEKTFHNTTLGELWSDVASEQLEHDILVNRREKYASQVPDGVVYITGGIDSQTSGRY
                     ECYVWGWGAEEECWLIDKTIVLGRYDEEDTLQRVDGVIRKQYRRSDGTTIGVSRWAWD
                     TGGIDAQVVYNRSLKLGPLWVIPIKGASSYGQPVVNMPRTRNANKVYLSLIGTDTAKD
                     LLAMRLQLEPDSKSATPGAIHFPNDDEIFSTTEAKQLVSEVLIPKLINGRVVYRWDNQ
                     GRRNEALDCWVYGLAALRISKIRFQLNLETLAEQRKKSQNKLSLEEMARMLGGS"
     gene            2817..3050
                     /locus_tag="QBH27_003050"
     CDS             2817..3050
                     /locus_tag="QBH27_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015331.1"
                     /translation="MTSREVLTERLLEAEIALHKLLTGKSTVSLSHGDSAGNNRSYQY
                     SQASIEQLRTYIIELKSQLGLSTGRRRPVGVRL"
     gene            3047..4621
                     /locus_tag="QBH27_003051"
     CDS             3047..4621
                     /locus_tag="QBH27_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ7015332.1"
                     /translation="MTAPQQLLGPDGKTPLRRYAGYNGGGPGFGGQLIDWNAPQQSAD
                     AALLPNFYRGNARADDLVRNNGVASNAVQLHQDHIVGNLFKLSYRPNWRYLGISREDA
                     RALARDVEVAWTEYAEDPHCTIDIERKRTFTMMIREGVATHAFNGETCVQPVWESSAG
                     SVFRTRFKMVSPKRIRNPGYAADTQFRRAGVDIDKNGAAVGYWIAEDTYPLGGVGKCR
                     RIPAQLSSGRHAFIHIFEPLEDGQTRGDNIFYSVMERLKMLDTLQQTQLQSAIVKAMY
                     AATIESELDSQQAFEYIAGAGTDIDSNPLNSFIQSYVTYYNGANIKLGGVKVPHLHPG
                     DKLSLQTAQNADAGFSSLEKSLLRYVAAGVGASYEELSRDYSQVSYSSARASANVSWR
                     FFMGRRRFIAARQASLMFCCWFEEALARGIITLPRSAVRSFYEARNSWTNALWIGAGR
                     MAIDGLKEVQESAMRITTGLSTYQNELALQGQDYEEVMEQQEYEIQRRREMGLSEPSW
                     SVSHPSNTNDNGWGGN"
     gene            4621..5928
                     /locus_tag="QBH27_003052"
     CDS             4621..5928
                     /locus_tag="QBH27_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDQ7015333.1"
                     /translation="MPWNNFPHLAARAFNQPLLLEPTYARVFFSALSDRFGTGRLIDT
                     ASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMS
                     GYNGIAKRLQQAISDPDVRGVLLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
                     ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDG
                     NPYSQLPGDVRDEFQLSINSTREQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGL
                     ADQLVNYADAIAVMADALKPKMERFMSGTETTAETTTTEQTATTTTVAQVEFNTEQIR
                     ADAASSELARVMAIINCPEAVGREAQAKALAGVPGMTVGQAQVVLAAAPKTAQARTET
                     ALDTLMSTESPATIQDAGSTTATGTTANVSMLVAAGRSILGDE"
     gene            5928..6257
                     /locus_tag="QBH27_003053"
     CDS             5928..6257
                     /locus_tag="QBH27_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDQ7015334.1"
                     /translation="MTTETYSPDDFILGPDLVVTTVGHFQGGINVPRLTPIMLDATVG
                     TFKVWDGSVGKAIGLTATAVNTGSSSADCSYYKSGSFRYTAINWGTVTDVAKRKSAFA
                     GTPISVG"
     gene            6316..7350
                     /locus_tag="QBH27_003054"
     CDS             6316..7350
                     /locus_tag="QBH27_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDQ7015335.1"
                     /translation="MSDSFTTSELITATQQVFKFNPLFLKLFFRETYTFTSEEVFLDK
                     IPGKVNMAVYCAPMITGKVDRTRGYSTNHFKPGYTKPKHTINPNMSIKRAAGEQIGQP
                     ETPVERRAKIIMQNLLDEELSISQLEEFQAVQAVLYGKYTVSGSNIETYEIDMSRSAT
                     NNVTQSGSTAWSTQDAETYDPSDDIESYADLASGAVNVIIMDGKAWKQLKRFKKFWTA
                     LDTRRGSNSQLEVALKNLGDVVSFKGYYGDTALFVYKGQYLDPVTDTETRYMPDNTMI
                     LGNTKNRGLRTYGAIQDEDALKEGISEATRYPKVWTTTGDPAVTQTMTQSAPAMVLTD
                     ADAFVVVKIA"
     gene            7385..7804
                     /locus_tag="QBH27_003055"
     CDS             7385..7804
                     /locus_tag="QBH27_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDQ7015336.1"
                     /translation="MSTKTELLARIDDLSAQLGRELPRSGTIAELESIVAGAESELDI
                     LNEQSGDAGGIEITVNTASGTDNELAQSIASAIVSQSQEQELAPATRRVKLRNTLDVY
                     HYVNGRRVREIVAAGREIVVDSPEVADLIAADHVYAL"
     gene            7801..8181
                     /locus_tag="QBH27_003056"
     CDS             7801..8181
                     /locus_tag="QBH27_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015337.1"
                     /translation="MSYYDDLRAGDEEMIREWGRPVKLRGKTDPIIAIFNEPYARVDV
                     PHAGFITGTVTSLTALSDDVAGVVARDVVQVPKQRSIEPDGSVTWSGWTDYVVKEPQP
                     DGTGLTNIFLEPHTSSENSEYSKY"
     gene            8213..8893
                     /locus_tag="QBH27_003057"
     CDS             8213..8893
                     /locus_tag="QBH27_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015338.1"
                     /translation="MADLRSNAQMFDIDVSALEQLRVEISATQHQMLMVYNRALNRTA
                     KHMHRISAGMILTALAAKNHKAVDKRIKPFIKRRNFTKEGAGDLSSAKLWYGLNDFRV
                     SELKGRLQNPRRQKQPRDLETGLFLKTKKGARGATFTPKSAGLAMMSWPDSFVAKRYG
                     AKSVWIRLDRGGIEEARVPVHDALEDAIDDYIFENIGPVFMGFFEKDLRGRVKGNVHV
                     DPKTGKRL"
     gene            8890..9426
                     /locus_tag="QBH27_003058"
     CDS             8890..9426
                     /locus_tag="QBH27_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015339.1"
                     /translation="MSGLDAFDEYLDRVKGAVLQIPFIKTFGIYPEIPAGFETPALFL
                     EISNWSQSDESVPGSIQSVELSCNLYLLREFAADQYGLKSQNAALYMTSWINGRMFGP
                     GTKPAKFSDAEPCDWIKNGQSVGSHSVQCVSFTQVVGVGPDIFDYPSQGTLKNIYVGI
                     APDIGAEHEGDYYGPIGR"
     gene            9407..10309
                     /locus_tag="QBH27_003059"
     CDS             9407..10309
                     /locus_tag="QBH27_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDQ7015340.1"
                     /translation="MAQSDDEYAAAENARRLRDAVKRGTIAAVKMNPPRCRVSFGGEH
                     QSGWLQWFTHATSERVDWSAPSVGDPVTVVSEGGDTRNGVVMLGLHIDDKAPPSNDPH
                     DHVTAYCDGATMTYNTKNHTLTWQGVPDGVVKILGESEIEIFGRADVTINSENVVNIH
                     GGKLINADADIINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPGGI
                     TLAGPTHITETLVVDKLATFRNDISVTGDNGGTGNITTRGSVLAGQEVQDRQGTITEV
                     RTTYNGHTHICPDGETQQPNQPMA"
     gene            10312..10653
                     /locus_tag="QBH27_003060"
     CDS             10312..10653
                     /locus_tag="QBH27_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDQ7015341.1"
                     /translation="MLGMDRNTGKLLSGTDHIRQSIVDILTTPLGTRVMLPEYGSKLF
                     DLVDNPTDPSLAMRIIMESAGAIARWEPRVRIDRINVLAVDIGKITILIIATDIETQQ
                     RLEFNNMELIF"
     gene            10650..11570
                     /locus_tag="QBH27_003061"
     CDS             10650..11570
                     /locus_tag="QBH27_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDQ7015342.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPAFVKTPLFSDVKSNLLSELQIL
                     YPQFNALLESDPAVKLLEIVAYREIIITARVNQGMLAVLLAFAKGSDLDQIGANFDCL
                     RLLITPANPDVIPPTEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDMYVLSRTGDGTPPQSLLNTVNAALSPDDTRPLTDFVTVKPASN
                     LNYRVEAVIVAGLGPDQNVLLNGAQSDLAIYVDTQHKIGATAALSGIYDAIHRDGTER
                     VILISPTEDVIAGVGQAPYCTEIKLSVQMG"
     gene            11573..12199
                     /locus_tag="QBH27_003062"
     CDS             11573..12199
                     /locus_tag="QBH27_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDQ7015343.1"
                     /translation="MTSQSVLPPNAITPERALEVVLSHVGDLPGDIRIIKNPDLCPAN
                     LLPWLAWEYAVTYWNPDWSEQQKREIIKAAAWQNKHRGTRGAVERALLTVGFESKMRE
                     WFEATPRDDPYTFAIKIYLLKSMGLDLELLNTFIAQIFDAKNCRSLLKEINFETGVDG
                     EFFIAGTPYAKIGVNVPADGDGGVKINGGLYISGSPIVSLNVEIGPNG"
     gene            12192..13376
                     /locus_tag="QBH27_003063"
     CDS             12192..13376
                     /locus_tag="QBH27_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015344.1"
                     /translation="MAENKIYAVLTDRGAQLEAAALASGVPVVLNKFVIGDANGNDDV
                     TPDPARTALIHETYRGDIKSAENSGNQVIFTLYVPPDTGGYTIREVGILTDKGELYSV
                     ARSPDILKPTDSNGALISITYKYTLAVSSTSTVNVVVYDNYVTPEEADKKYLQISKNL
                     SEIPANGETAQQAARKNIGIDGDVAYRDKGNAFTHPNTFKDDVIFEDVLSSKKQININ
                     KPGTDLTLFSFAGLSDVSGDYLDFSTRGENNQKVFYRLRGVHYDGLVLLCGGQTYKYW
                     NEANLKPVKTINGESPDENGNIDISLSPDPSPEIGVVGAVGTYALVAINHAPQILPGS
                     IWAGGDLYYASINNTGSYNTDIATKPFYDTPLIGSWRAMAYSPGGVGDIGTACFLAIR
                     IA"
     gene            13376..13765
                     /locus_tag="QBH27_003064"
     CDS             13376..13765
                     /locus_tag="QBH27_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015345.1"
                     /translation="MISVDSFRKITNPVFNDEHGLSINIICDIDGIGNDIPFTAMKND
                     CEEHGRVLYERALSGDYGSISKFVKKEQTQKLQTTLRTVGDLSIEAFSIQCAVDAGTA
                     SDAQIENLAELKEAIATRIGENREGIK"
     gene            13762..15264
                     /locus_tag="QBH27_003065"
     CDS             13762..15264
                     /locus_tag="QBH27_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDQ7015346.1"
                     /translation="MSVTNFLHGIRTLEYDDGTKEINTVDISVIGIVGTAPDSSYPTC
                     ASLLWGSELADNLVEFSTVMPGADGNNWIVEIVDLGINGNVATPGYSTLPDGSRKLTM
                     ITDGAMTPSKMYDQNQQYKEGLQIGEYINVTFGGDHAGTGTVFALPPTNLSGGKDESF
                     KYNVPTLIAGSQKKAGLLGGSGTLPPAVSEILNQEDAIIVVVRVEEDSDETKMRQNVV
                     NGINALLTSAQINQVTPRILIAPDYSANDYIAEQLEVVTNKLRGVGYIDSPRGATPAD
                     VVNRRQRYGGRMEILRPRVYSTSDVSGLSRPYSAIAAGLRARIDNEKGFWWSKSNQNI
                     YGITGLEQVDDFIIGETNCTANLLNASQVSTIIRYDGFRHWGNYLCSLSPQWSFECVR
                     RTADVIEDSIARAMMTDFIDRPIDLHLGTDVVESINAYLHKLEEQGAINGGRAWLDEE
                     LNTKESLAAGNLYINVDFGPKSPAQTITLMYRINNDYTVEALASLFKETV"
     gene            15282..15794
                     /locus_tag="QBH27_003066"
     CDS             15282..15794
                     /locus_tag="QBH27_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDQ7015347.1"
                     /translation="MADSNTYRAFALFVQGERVLNCTEYTPVDMKIIEDDFKTGAMDT
                     AITLDGGMEKMSASFKVSGSDSGVMGYLGLIPGAKTRFEIRSAYTDNYGVNFERIDTY
                     EGLITAITDDAQGTDSKSAVGQSVTIAPSYYKRVQNGKIIYEIHPAKMKRVINGVDVL
                     AGVARILHVY"
     gene            15807..16088
                     /locus_tag="QBH27_003067"
     CDS             15807..16088
                     /locus_tag="QBH27_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDQ7015348.1"
                     /translation="MESLLDSMTITLSRPFIIKGESRDTITIREPKLRDRIMFSNDKS
                     GLEERTATMLARLANLEREDLYALPACDYDQMEAAFNELVKPPKDRHQI"
     gene            16197..17837
                     /locus_tag="QBH27_003068"
     CDS             16197..17837
                     /locus_tag="QBH27_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015349.1"
                     /translation="MAISQNFKTQVVFGGRIDPSFRRGTTELNDAIRQTSSTVGKLTK
                     SQDKLKDKIAAMKLAGKDVSDLSAQYQKLDRRIKATTQDQEALNTQLAKKQRLEKWTG
                     RAKGAAKWGGRAAAGAVRATGRGIKWGTLGAAGLIGGAAAGALAMNAETSEKLGLAKS
                     YGVGVEKYAAWENIGKAAGLNGENIGDLSEELTNKIGEIGNEKSLNPMLFQIGLTKKR
                     MAGWDREKQFNEVMRRISEMKDEKQAASLADQLMGGEANKIMTYMKATGKSWEQTMSD
                     AQKSNLLTKEGAEGAARAHVSVTNLWGSITSGLADTLGKIGGELAPTFDSVRETFTSW
                     FKDNQGGFVSTITEWVKPENMKKMWDGIVNFGEGCVKFGKIIWAVVKKLEWLIPDEKS
                     DEEQKTYNDEYNRAYQEFMDSGGKYSPNAGLTADNIAKAKAEEAVENMRHPERQEQAK
                     SQAESMLAFVNPLSGMVNKNTAPAALDSSTLNIDTLKQAVSTPAPEQNNKIEINIVGA
                     TDPQSIQQSAASGVLDGLRQAASSYNRGAMFDKPAAAG"
     gene            17873..18262
                     /locus_tag="QBH27_003069"
     CDS             17873..18262
                     /locus_tag="QBH27_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015350.1"
                     /translation="MLGLGDFIFAISTVAYNKLQRSDAWRWAQQTRFGKNDALQITGR
                     PNPTITIDGKINALFLDGCGVGLLTDLRALGNTGEPQQLVLGTGEVKGYWVLRELTET
                     QNSFLMGGTPKSQDFSLTLEYYGASLD"
BASE COUNT         4976 a         4194 c         4924 g         4180 t
ORIGIN      
        1 gctcattaag gcttcaattt tttctctgtt cattttttgc gttctcatgg ttgttgtttt
       61 tctgtaatga tatcaataag ttcaagatat atacatatat ggaaaacttg ttcggtagtc
      121 tatcgataca gcacaaaagg atggctcgct gccaaagtga aagcttacgc tgataatcag
      181 atacacaaag ctgatgtatg caaacaagta cgccgcgagg ctacagttga gtttgatgaa
      241 cctgatgcga gcccctaatt cctgtcctga aagggagata gccccctggt cgcgggtcct
      301 ttccgaaatc caaaacactg agggtcggta gacgcgcaaa aactcgctcg tttttagtat
      361 tttttcattt tgggtatttc cggttccggt gaggattttt aatggcaagt caggctgagg
      421 ttgcagcaca tttactgcta tctgatcgtc gtctgcgcga tctcgcaaaa cttccgggag
      481 caccagtccc acaaggacgt ggtgattggg agctagacgc atggcgccat ttttatattc
      541 gttatctccg gagcaataga cgcgacacaa ctagtaatga cgaaccggaa gcgggggaca
      601 attctcccga aaaaaatcgc gagcagtggc tgaaaaatga ggaacgacag gagcgaatcc
      661 tgatggcacg agtaaaacgc cgcattctcg ctaaacgcta cgcgccaatt gaattaatta
      721 gcgtcgctgt atctcgcgtc gccgttgaat tacgtacccg cgtcgagtcg tggccaccac
      781 gcctgaaaaa ggtctggccg gagatgccgc aagaggcgag cagtgtatta cgagaggagc
      841 tggcgatagc cttaaatgag ttgtcagaca tacgaatcga cttcagcgat tacgatgtca
      901 gcgatatcga acgcgatctc gacagggttg aatcccttgc gcgtgacgat accgatgacg
      961 ggtgttgagt gggctgataa atatttttat ctccctgagg gctccagcca catcgctggg
     1021 cactggacga ctcagccggt ccaggtagtg atgctcaata tgatgacgaa cgacgcgata
     1081 aaaatcgtgt ctgttcgcaa atcagctcgt ctcggttata caaaaatact cgtcgcggcg
     1141 ctgctctatt tcgctgagca caaaaaacgt agtgccgtgg tctatcagcc tatcgatgac
     1201 gaatcggatg gatttgtcgc cgacgaggtt gaccccgcta tcgccgaaat gccagtgatt
     1261 cagaaaattt tccccgactg ggataaaagc aacgagcgta acaatctcca gcgtaaagaa
     1321 atgagcggcg cgattcttga ttttcgcggc gcgagtgcac caggaaattt ccggcgacta
     1381 acgaaacagg ttgtcgaggg tgacgaagtt gacggctggc cgctggaagt tgccaaaaaa
     1441 ggcaaaggcg agggctcgcc catcgaactg gcgctcgttc gaattaaagg ggcagcgtac
     1501 ccgaaggcga tttttggttc gacgccgacc gttaccggca aaagtcatat cgaaatgctc
     1561 gaagacgctg ccgatctgac gtttcgtttt tacctgacat gtccgcattg tggcgaggag
     1621 caggtcctgg tatttggttt cgacggtatc gaatacggcc tcaaatggga taacagcctg
     1681 cagaccaatg aggcgaaatc gtcgtccgcg tattaccagt gctgccactg cccggaacat
     1741 ttttactatc gcgatctcga aaaaatggag ttcggggggc gctggatagc cgaggactgc
     1801 acctggaccc gagacggcat tcattttttt gatcatgacg gtggcgtcgt tcgcgcgccg
     1861 aaacacgctg cgatcgtgat aaacgccctg tattcactaa acctcgatgg atggggcgag
     1921 attgtcagcg agtggctgaa agcgaaaggc gacccgctca aggaaaagac gtttcataac
     1981 acgacgctcg gcgaactctg gagcgacgtg gccagcgagc agctggagca cgatattctg
     2041 gttaatcgcc gggaaaaata cgccagccag gttcctgacg gtgttgttta tataaccggc
     2101 ggcatcgact ctcagacgtc cgggcgctac gagtgttatg tgtggggctg gggagccgag
     2161 gaggagtgct ggctgattga taaaacaatc gtcctcggtc gctacgacga ggaggacacg
     2221 ctgcagcgcg tcgacggagt gattcgcaaa caataccggc gcagcgacgg gaccacaatc
     2281 ggcgtcagtc gctgggcgtg ggataccggt ggtatagatg cgcaggtcgt ttataaccgc
     2341 tcgctgaaac tcggtccgct gtgggtcatt ccaatcaagg gcgcgagctc atacggccag
     2401 ccggtcgtaa atatgcctcg tacccgtaac gcgaataaag tgtatttgtc gttgattggg
     2461 acagatacag cgaaagacct gctcgcaatg cgcctgcagc tggaacccga ctccaaatcg
     2521 gcgacaccag gtgcgattca ttttcccaac gacgacgaaa tattcagcac gacagaggca
     2581 aaacagctcg tctccgaggt tctgatcccg aaactcatta acggtcgcgt cgtttatcgt
     2641 tgggacaacc agggccggcg aaatgaggcg ctcgactgct gggtgtacgg actggcagcg
     2701 ctgcgtatca gtaaaattcg tttccagctc aatctcgaga cgctcgctga gcaacggaaa
     2761 aaatcacaaa acaaactgtc tctcgaggag atggccagaa tgctcggagg gagctaatga
     2821 cgtcgcgcga ggttttaaca gaacgactgc tggaagcgga aatcgctctg cacaaattat
     2881 taacgggtaa atcgaccgtg tcgctgtctc acggcgattc agcaggaaat aaccggagct
     2941 atcaatactc gcaggcgagt attgagcagc tccgaacgta cattatcgag ctgaaatcac
     3001 agctcggtct gagtacggga cgccgtcggc ccgtgggagt tcgattatga ctgctccgca
     3061 gcagctgctc gggcctgacg gtaaaacgcc actacgccgt tacgcggggt ataacggcgg
     3121 cggtcccggt ttcggtggcc agctgattga ctggaacgca ccacagcaga gcgccgacgc
     3181 agcactgttg ccgaattttt atcgcggtaa cgcgcgagca gacgatctcg ttcgcaataa
     3241 cggcgtcgcg tcgaacgccg tgcagctgca ccaggatcat atcgtcggca atctgtttaa
     3301 gttgagttat cgccctaact ggcgttacct cggcatatct cgcgaggacg ccagggcgct
     3361 ggcgcgtgac gtcgaggtag cgtggactga atacgccgag gaccctcact gcacgattga
     3421 tatcgagcgg aaacggacgt tcacaatgat gatccgcgag ggcgtggcta ctcacgcttt
     3481 caacggtgaa acctgcgtac aaccagtctg ggagagtagt gccggcagcg tttttcggac
     3541 gcgattcaaa atggtctcac cgaaacggat tcgaaatccg ggttacgcag ctgacactca
     3601 atttcgccgc gccggtgttg atatcgataa aaacggggcc gcggtcgggt actggatagc
     3661 cgaggatact taccctctcg gcggagtcgg taaatgccgg cgtatcccag cacagctcag
     3721 cagcggcaga cacgcattca tccacatatt cgagccgctc gaggacggac agacccgcgg
     3781 ggacaatatt ttttacagcg tcatggagcg cctgaaaatg ctcgacacac tgcagcaaac
     3841 acaactgcag agcgccattg tgaaggcgat gtatgccgcg acaatcgaat cagagctcga
     3901 cagccagcag gcgtttgaat atatcgccgg cgcgggtact gatatcgact cgaacccgct
     3961 gaattcgttt attcagagtt acgtgacgta ctacaacggc gcaaatatca agctcggtgg
     4021 tgtgaaagtt ccccacctgc atcccggcga caaactcagt ttacagaccg ctcagaatgc
     4081 tgatgctggt tttagttcac tggaaaaatc actcctgcgt tacgttgcag ctggcgttgg
     4141 cgcgtcatac gaagaactta gccgggacta cagccaggtc agttactcca gcgcccgggc
     4201 cagtgctaac gtcagctggc gatttttcat ggggcgtcga cgttttatcg ccgcgcgaca
     4261 ggcgtcgctg atgttctgct gctggtttga agaggcgctg gcacgcggaa ttatcaccct
     4321 gccacgctca gctgtccggt cgttttatga ggcgcggaac tcctggacga acgcgctctg
     4381 gatcggcgct ggtcgcatgg cgattgatgg gctgaaagag gttcaggaga gcgccatgcg
     4441 catcacaact ggcctcagca cgtatcagaa cgaactggcg ctgcagggac aggattacga
     4501 ggaggttatg gagcagcagg aatacgaaat tcagcgccgg cgtgaaatgg ggctgagcga
     4561 accgtcatgg tctgtatctc acccctcaaa taccaacgat aacggctggg gaggtaactg
     4621 atgccgtgga acaattttcc gcacctcgcc gccagggcgt tcaatcaacc gcttttgctg
     4681 gagcccacct acgcgcgggt atttttttcg gcgctgagcg accggttcgg taccgggcga
     4741 ctgattgata cagcgtctgg agaggtaatg aacagcgacg aaatgaacgc gctcgcgatg
     4801 gggtgggaca gcagcgagcg aacacgccag aaatcgtatc gcgtggagcg tggtatagcc
     4861 gttctgccgg ttaccgggac gctggttcat aaattgggtt atatcaaccc agttagcggg
     4921 atgagcggtt acaacggaat cgcaaaacgc ctgcagcagg cgatttctga tcccgatgta
     4981 agaggggtcc tgcttgatat tgactcccct ggcggtgaag tcgccggcgc gtttgatact
     5041 gctgatttaa tcgcccgggc gcgagagcaa aaacctgtgt gggcgctggc cagcgatacg
     5101 gcctgcagcg ctgcctattt gctggcgtca gcgtgttcgc gccggctgat aacgcagaca
     5161 ggcacagttg gttcaatcgg tgtcctgatg gctcaccgct gcgtcgaaaa agcgctggag
     5221 attgctggcg tcgacgtgac gctgatttac gccggcgcgc acaaagttga cgggaacccg
     5281 tattcccagc tgccaggcga cgttcgcgac gaattccagc tgagtattaa cagcacgcgc
     5341 gagcagttcg cgcaaaaagt ctcggattat accgggctga aaaaatccag ggtgctggcc
     5401 acggaagctg cagtatttat cggcgcggac gcgattaaat ctggtctcgc tgatcaactc
     5461 gttaattacg cggacgctat cgcagtgatg gccgacgcac tgaaaccaaa aatggagcgc
     5521 tttatgtctg gtacagaaac cacggcggag accacgacca cagaacaaac cgcgaccacg
     5581 actactgtcg cgcaggttga gtttaacacg gagcagattc gcgcggacgc cgcatcaagc
     5641 gaactggcgc gtgtgatggc catcatcaat tgtcccgaag ctgttgggcg cgaggcgcag
     5701 gcaaaagcgc tcgctggcgt ccccgggatg acggtcgggc aggcgcaggt agtcctcgct
     5761 gcagcaccta aaacggcgca ggcgcggaca gaaacggcgc tcgatacact aatgagcact
     5821 gaatcaccag cgactattca ggatgctggc agtactacgg caacaggaac aaccgcaaac
     5881 gtctcgatgc tggttgctgc agggcgttca attttagggg atgaataatg actacagaaa
     5941 cgtatagccc ggacgatttt attcttgggc ctgaccttgt tgtcacgacc gtcggtcatt
     6001 ttcagggcgg gatcaacgta ccccggctga caccgattat gctcgacgct actgtcggga
     6061 cgtttaaggt ttgggacggc tcagtcggta aagctattgg cctcactgcc accgcagtta
     6121 atactggctc cagttctgca gactgctcgt actataaatc aggctcgttc cgttacacgg
     6181 cgattaactg gggtacggtt accgacgtag ctaaacgcaa atcagcattt gcgggtacgc
     6241 cgatcagcgt cggctgatag caaaaaactc aaaacaagcc gcctccgggc ggttttttta
     6301 taacaggata tataaatgag cgattcgttt actacgtcag aactgattac cgcgacacag
     6361 caggtattta agttcaatcc gttgttttta aagctgtttt tccgtgagac ctacacgttt
     6421 acgagcgaag aggtttttct ggataaaatc ccgggaaaag tgaacatggc cgtgtactgc
     6481 gcgccgatga tcaccggcaa agttgaccgc actcgcggct attcaacgaa ccatttcaaa
     6541 ccgggttaca cgaaacccaa gcacacgatc aatccgaata tgagcatcaa gcgcgcagcc
     6601 ggtgagcaga ttggccagcc ggaaacgccg gtcgaacgtc gtgcaaaaat catcatgcag
     6661 aacctgcttg atgaggaact gagcatcagc cagctcgaag agttccaggc ggtacaggct
     6721 gttctgtacg gtaaatacac tgtttccggc agcaatatcg agacctatga gatcgacatg
     6781 agccgcagcg cgacgaataa cgtcactcag tccggttcga cagcctggtc gactcaggac
     6841 gcggaaacgt atgacccgag cgacgatatt gaatcctacg cggacctcgc ctccggtgcg
     6901 gttaacgtga tcatcatgga tggtaaagcc tggaagcagt tgaagcgttt taaaaaattc
     6961 tggacggcac tggatacgcg ccgtggctca aacagccagc tcgaggtcgc gctgaaaaac
     7021 ctgggcgacg ttgtcagctt taaaggctac tacggcgaca ctgcgctgtt tgtctacaaa
     7081 gggcaatacc tcgacccggt aacagacact gaaacgcgtt atatgccgga taacacgatg
     7141 atccttggca acacaaaaaa ccgcgggctc cgcacttatg gcgcaattca ggacgaagac
     7201 gcgctgaaag agggtatttc cgaagcgacg cgctatccaa aagtctggac cactaccggt
     7261 gatccggcag tgacgcaaac aatgacgcaa tccgcgccag caatggtcct cacggacgcc
     7321 gacgcgttcg ttgtcgtaaa aatcgcgtaa gagtcgaaag gctctttttt gttaaggaat
     7381 caacatgagc acaaaaacag aattgctggc gcgtattgac gatctgagcg ctcagctcgg
     7441 tcgcgaatta ccgcgcagcg ggactattgc ggaactggaa tcaatcgttg ccggcgctga
     7501 gtcagagctc gatattctga acgagcaatc cggtgacgcg ggaggcatcg agattacagt
     7561 gaatactgcg tccgggaccg ataatgaact cgctcaatca atcgcgagcg ctatcgtttc
     7621 acagtcacaa gagcaggagc tcgcaccggc tacgcgccgc gtcaagttgc gtaacacgct
     7681 ggacgtttat cactacgtga acgggcgtcg cgttcgcgag attgttgccg caggccgtga
     7741 aattgttgtt gattcaccgg aggtcgcaga ccttatcgca gctgatcacg tttacgctct
     7801 atgagctact acgatgacct ccgggccggc gacgaggaga tgatccgtga gtggggacgg
     7861 cccgttaaat tacgcggtaa aaccgacccc attatcgcta ttttcaacga gccttacgcg
     7921 cgcgttgacg ttcctcacgc tggttttatt accggtacag taacgagcct gacggcgctc
     7981 tcggacgacg tcgctggcgt tgttgcgcgt gacgtcgtcc aggttccaaa gcagcgcagc
     8041 atcgaacctg acggctctgt tacctggtct ggctggaccg attacgtggt taaagagcct
     8101 caacctgatg gcacagggct cacgaatatt tttttagagc ctcacacgtc cagcgaaaac
     8161 agcgagtatt caaaatacta agtgggacct cggtcccact taagggggaa ccgtggccga
     8221 tttacgtagt aacgcgcaga tgttcgatat cgatgtttcg gcgctggagc agctcagagt
     8281 cgaaatcagc gcgacgcaac atcaaatgtt aatggtatac aacagggcgc tgaaccgaac
     8341 tgcaaaacat atgcaccgaa tttcagcagg aatgattttg actgcactgg cagctaaaaa
     8401 tcataaggcc gtagataaac gaatcaagcc gttcattaag cgccgtaatt ttacaaaaga
     8461 aggggcagga gacctgagca gcgccaagct ctggtacggt ctgaatgatt tccgggtatc
     8521 tgagctaaag gggcgattac aaaatccacg gaggcaaaaa cagcctcgcg atcttgagac
     8581 cggcctgttt ttgaaaacca aaaaaggcgc tagaggggca acttttacac ccaaaagtgc
     8641 agggctggcg atgatgagtt ggcccgattc attcgtagcg aaacgctacg gtgcgaaaag
     8701 tgtctggatt cgactggatc gcggaggaat cgaagaggct cgcgtacctg tgcacgacgc
     8761 gctggaggat gctatcgatg attatatttt tgaaaacatt ggccctgttt ttatgggctt
     8821 ttttgagaaa gatttgcgcg gtcgcgtgaa aggtaatgtc cacgtagacc caaaaacagg
     8881 taaacgatta tgagcggact ggatgcattt gacgaatatc ttgatcgcgt taaaggcgcg
     8941 gttttacaaa taccgttcat caaaacattc ggaatttatc cggaaattcc ggctggattt
     9001 gaaacgccag ctctgttcct ggaaatcagc aactggtcac agagtgacga atcggttcca
     9061 ggctcaattc agtcagtcga gctgtcgtgt aatttgtatt tgctgcgtga atttgcagct
     9121 gatcagtacg gactgaaatc gcagaatgca gcgctctata tgacgagctg gatcaatggg
     9181 cgaatgttcg gtcccggaac taaaccggca aaattcagcg acgcggaacc gtgcgactgg
     9241 attaaaaacg ggcagtcagt cggttcgcat tcagttcagt gcgtgtcgtt cacgcaggtt
     9301 gtaggcgttg gtccagatat tttcgattat ccctcacagg ggaccctgaa aaatatatac
     9361 gtcggaatag caccagatat cggcgcagaa catgagggcg actattatgg cccaatcgga
     9421 cgatgaatat gctgcagcgg agaacgcgcg caggctgcgt gacgcagtta aacgcggcac
     9481 gatagctgca gttaaaatga atcctccacg ctgccgagtc tcatttggtg gcgaacacca
     9541 gtcaggctgg ctgcagtggt ttactcacgc cacctcagaa cgcgtcgact ggagcgcacc
     9601 atcagtgggc gatcccgtta ctgttgtttc tgagggtggg gatacgcgga acggtgtagt
     9661 tatgctcggg ctgcacattg acgacaaagc tccgccaagc aatgaccccc atgatcatgt
     9721 cactgcatac tgtgacgggg ctacgatgac gtataacaca aaaaatcaca ctctgacatg
     9781 gcagggcgta ccggacggcg tggtaaaaat actcggtgag tctgaaatag aaatatttgg
     9841 acgcgcagat gttactatta atagcgaaaa tgtagttaat atccatggtg ggaaattaat
     9901 taacgcagac gctgatatta ttaatgtcac agcgacagat acaattaacg cacatgctga
     9961 tttagtgaac gttatagcaa cgagttctgt tagtgttact gctgcgaaca gaatatcgct
    10021 gacagctcaa acaatcagcg cgtgggctcc gggtggtata acactagctg gtccaacgca
    10081 tattaccgag acattagtag tagataaatt agcgacattc cgtaacgata tttctgtcac
    10141 tggagataac ggtggaacag gtaatatcac aactcgcggt agtgtgttag cagggcaaga
    10201 ggtgcaggac cgacaaggca caataactga agtccgcaca acgtataacg gacacactca
    10261 tatatgtccg gacggggaaa ctcaacaacc gaaccaacca atggcgtaaa gatgcttgga
    10321 atggaccgta acaccggtaa acttttatcg gggactgatc acattcgtca gtctatcgtt
    10381 gatattttaa cgaccccgct ggggacgcgt gtaatgctgc cggaatatgg cagcaaatta
    10441 tttgatcttg ttgataatcc cacagatccg tcgcttgcta tgcgaataat catggaaagc
    10501 gctggcgcaa tagcacgatg ggaaccacgc gtcagaattg acagaataaa tgtgttagca
    10561 gtggatatcg ggaaaataac aatattaatt atcgcaacag atatcgaaac acaacagcga
    10621 ttagagttta ataatatgga gctgatattt tgataacatc aacagttcag aattcgatag
    10681 tgaaaactat tgatatgagt ctgctgccgc cgccagcatt cgttaaaacc ccgttatttt
    10741 cagatgttaa atctaatcta ctgtcagagc tgcagatatt atatccacaa tttaacgcac
    10801 tcctggagtc agacccggcc gttaaactgc tggaaatagt tgcatacaga gaaatcatta
    10861 ttacagctcg ggtaaatcag gggatgctcg ctgtattgct cgcgtttgca aaggggagtg
    10921 acctcgacca gattggcgct aattttgact gcctgcggct gttgataacg ccagctaatc
    10981 ccgatgtaat tcccccgact gaggctgttt acgagagtga cgacgagtat cgacaccgaa
    11041 tacagctatc atggtatgcg cggaatactg ccggcagtac gaacgcctat aactatttcg
    11101 cgctgtcgag tgatcccgac gttctgtcag ctcaggcgta cggcccgccg gtgacccagc
    11161 ctggatacgt cgatatgtat gtcttgtcgc gaaccggcga cggcactccg cctcaatcac
    11221 tactgaatac agtgaatgcg gccctgtctc ctgacgatac tcgaccgctg actgatttcg
    11281 tgactgtaaa acctgcgtcg aacctgaatt accgggttga ggccgttatc gtagccgggc
    11341 tcggacccga ccagaacgtt ttgcttaacg gtgctcagag cgatttggcg atatatgtgg
    11401 atacgcaaca taaaatcgga gcgacggcag ctctgtcagg aatttacgac gcgatacatc
    11461 gtgacggcac tgaacgtgtg attctgatat cgccaacaga ggatgtgatc gctggcgtcg
    11521 ggcaggctcc gtactgcaca gaaatcaaac tcagcgtgca aatggggtag ccatgacaag
    11581 tcaaagcgta ctgcctccga acgcaataac ccctgagagg gcgcttgagg ttgttttatc
    11641 tcacgtcggc gatctgcctg gcgatattcg aattattaaa aatcccgatt tgtgccctgc
    11701 aaatctgttg ccgtggctgg cgtgggagta cgcggtcacc tactggaacc ccgactggag
    11761 tgagcagcaa aaacgcgaaa ttattaaagc agctgcgtgg cagaataagc accgcggaac
    11821 gcgtggagct gttgaacgag cgttattaac tgttggcttt gagagcaaaa tgagagagtg
    11881 gtttgaagcc acgcccaggg acgacccgta tacattcgca attaaaatat atctgctaaa
    11941 aagcatgggg ttagatttag aactattgaa tacatttatt gcacaaatat ttgatgcaaa
    12001 aaattgtcgt tccttattaa aagaaataaa tttcgaaacg ggtgtcgatg gtgagttttt
    12061 tatagccggt acaccttacg cgaaaatagg cgtaaacgtc ccagcagacg gcgacggcgg
    12121 tgtaaaaatt aacggcgggc tatatatatc gggctcacca attgttagtt taaatgtgga
    12181 gatagggcca aatggctgaa aataaaattt acgcagtact gacagaccgt ggcgcacaac
    12241 ttgaagccgc tgcgctggca tcgggtgtac ccgtggtgct gaataaattc gttattggcg
    12301 acgcaaacgg aaacgacgac gtaacaccgg acccggcacg aactgcatta attcacgaga
    12361 cgtatcgtgg ggatattaaa tccgcagaaa atagcggtaa ccaggttatc tttacacttt
    12421 acgtaccgcc agacactggg ggctatacta tccgtgaggt ggggatatta acagataaag
    12481 gcgagctgta ctctgtggcg cgctcgccgg acattttaaa acctacggac agcaacggtg
    12541 cgctgatttc aatcacgtat aaatacacac tcgcggtgtc cagcacatct acggttaatg
    12601 ttgtagtata tgacaactac gtaactcccg aggaagctga caaaaaatat ttgcagataa
    12661 gtaaaaactt atctgaaatt cccgcaaatg gtgaaactgc tcagcaggct gcgcggaaaa
    12721 atattggtat agatggggac gttgcatata gagataaggg taatgcattt actcatccaa
    12781 atacttttaa agatgatgtg atttttgaag acgttttatc ctctaaaaaa caaataaata
    12841 taaacaaacc gggcactgat ttgacgttgt tttcatttgc tggtttatca gatgtgtcgg
    12901 gcgattattt agatttttcg acccgtggag aaaacaatca gaaggttttt tatagattac
    12961 gcggagttca ctatgatggc ctggtgttac tgtgcggcgg gcagacgtat aaatactgga
    13021 atgaggctaa tcttaaaccg gttaaaacga ttaacgggga gtcgccggat gaaaatggca
    13081 acatagatat aagtctttca ccggacccat caccggaaat aggtgttgtt ggcgcggttg
    13141 gtacttatgc gcttgtcgct attaatcatg caccacaaat actcccgggc tccatatggg
    13201 ctggagggga tttatattac gctagtataa ataataccgg ttcatataac acggatattg
    13261 ccacaaaacc attttatgac acaccactta ttggctcatg gcgagccatg gcgtattcac
    13321 ccggtggagt cggtgatatt gggactgcct gttttttagc tattaggatt gcgtaatgat
    13381 aagcgttgat tcatttagaa aaataacaaa ccctgttttt aatgacgagc atgggttatc
    13441 tataaatatt atttgcgata ttgacgggat tggaaacgac attccattca ccgcaatgaa
    13501 aaatgattgc gaggaacacg gtagggtttt atatgaaaga gcgctgagcg gggattatgg
    13561 gagcatttct aaatttgtaa aaaaagagca gacgcaaaaa ctccagacta cattacggac
    13621 tgttggcgat ctgtcaattg aggcattctc aattcagtgt gcagtagatg ccggaaccgc
    13681 atctgacgcg caaatcgaaa atttggccga gcttaaagaa gcaatagcga cgcgaattgg
    13741 tgaaaataga gaaggaataa aatgagcgta actaattttt tacacggtat tcgcacgctc
    13801 gaatacgacg acggcacaaa agagatcaac acagtagata tttccgtgat tgggattgtt
    13861 ggtactgctc ccgattccag ctacccaact tgtgcatcac tattatgggg atcagagctc
    13921 gcagacaatc ttgttgagtt ttcgactgtc atgccagggg ccgacggaaa caactggatt
    13981 gttgaaattg ttgatttggg aattaacggg aatgtagcaa ctccaggata cagcacactg
    14041 cccgacggtt cgagaaaatt aacaatgatc acggacggtg ccatgacccc gtcaaaaatg
    14101 tacgatcaga accagcagta taaagaagga ctccagattg gagaatatat taacgtcaca
    14161 ttcggtggtg atcacgcagg aacggggact gtattcgcgc tccccccaac aaatttatcc
    14221 ggtggtaaag acgaatcgtt caaatataat gttccgacgt taattgcagg cagtcaaaaa
    14281 aaagctggat tgctcggtgg gtctggaacc cttcctcccg ctgtatctga aatactaaat
    14341 caggaggatg ccattattgt tgttgtccgc gtcgaagagg acagcgacga aacaaaaatg
    14401 cggcagaacg tcgttaacgg gattaacgca ttgttgacgt ctgcgcaaat aaatcaagta
    14461 acgccgagaa ttttaatcgc tccagattat agtgctaacg attatattgc agaacaactt
    14521 gaagtggtga caaataaatt acgaggcgtt ggatatatcg attcaccgcg aggcgctacg
    14581 cctgctgacg ttgtaaatcg tcgtcagagg tacggcggca gaatggaaat tctgcgccct
    14641 cgtgtttatt ctacgagtga tgttagtggt ttatctcgtc cgtattcagc tatcgctgct
    14701 ggtttacgag ccagaattga taacgaaaaa gggttttggt ggagcaaatc gaatcaaaat
    14761 atctacggaa ttacaggact cgaacaagtc gatgatttta taatcggtga aacgaactgc
    14821 acagcaaatc tgctgaacgc cagccaggtc agcacaatta ttcgctatga tggattcaga
    14881 cactggggta actatctgtg tagcctcagt ccccaatggt cctttgaatg cgttcgtcgg
    14941 acagctgacg taattgagga ttcaatagcc cgggcaatga tgaccgattt tattgatcgc
    15001 ccgatagacc tgcacctcgg aacagacgtt gtagagtcga taaacgcgta tctgcataaa
    15061 ttagaggagc agggcgcgat taacggagga cgggcgtggt tagatgaaga gttaaataca
    15121 aaagaaagtc tggccgctgg taatctttat attaatgttg actttggacc gaaatccccg
    15181 gcacaaacaa ttacgttaat gtaccgcatt aataatgatt acacagttga agctctggcc
    15241 tctcttttta aagaaacagt ctaataatct ggagaattga tatggcagat agtaacacct
    15301 atcgtgcatt tgcgttgttc gtccagggag aacgagtttt aaattgcact gaatacactc
    15361 ccgtcgatat gaaaataatc gaagatgatt ttaagacggg cgctatggat acagctatca
    15421 ctctggacgg gggaatggag aaaatgtcag ccagttttaa agtctctggc tccgattctg
    15481 gtgtgatggg gtatttggga ttaataccag gtgctaaaac aaggttcgaa attcgaagtg
    15541 catatactga caattacggt gtcaattttg aacgtattga tacatatgag gggcttatta
    15601 cggcgattac tgatgacgcg caggggactg attcaaaatc agcagttggg caatcagtaa
    15661 cgattgcgcc gagctattat aaacgagttc aaaatggaaa aattatttac gagattcatc
    15721 ctgcaaaaat gaaacgagta attaatggtg ttgacgttct tgcgggcgtt gctcgtattc
    15781 tccatgtata ttaaaaggca aataaaatgg aatcattatt agacagtatg actattacgc
    15841 tctctcgccc gtttattatt aagggggaga gccgcgatac aattactatt cgcgaaccaa
    15901 aattacgcga ccgcattatg ttcagtaacg ataaaagtgg tcttgaagag cggacagcga
    15961 caatgctggc gcgcctggca aatctggagc gtgaggattt atatgcactg cctgcgtgcg
    16021 attacgatca aatggaggcc gcatttaacg aactggtaaa gcctccgaaa gaccgacatc
    16081 agatatagtt attctgattc cgtttattgc gaaaaaactt gccatcccgc ccgatacgca
    16141 aatggacctg ccgtaccggg tttttaattt ctatgtaaac gaggtcatga aaatcgatgg
    16201 ctatttctca aaactttaaa acgcaggttg tattcggtgg gcgaatagac ccgtcgtttc
    16261 gtcgcggtac aacagaactc aatgatgcga ttcggcagac gtcgtctact gtcgggaaat
    16321 taacgaaaag tcaggataaa ttaaaagaca aaatcgcagc aatgaaactg gcaggtaaag
    16381 acgtttctga tttatctgct cagtatcaaa agctggaccg tcgaataaaa gcgacaacgc
    16441 aggaccagga ggcgctgaat actcaacttg ctaaaaaaca gcggctggag aagtggacgg
    16501 gtcgcgcaaa aggagctgca aaatggggcg gacgagccgc cgcaggtgcg gtgagggcta
    16561 cggggcgggg tatcaaatgg gggacacttg gcgcggcagg gctcattggc ggtgctgcag
    16621 ctggtgcgct ggcaatgaat gcagagacgt cagaaaaact cggtctggcg aagtcctacg
    16681 gcgttggcgt cgaaaaatac gctgcgtggg aaaatattgg taaagccgcg ggcctgaacg
    16741 gcgaaaatat cggggatttg tctgaagaac ttacaaataa aatcggcgaa atcggaaacg
    16801 aaaaaagcct gaatcccatg ttatttcaaa ttggcctgac gaaaaaacga atggctggct
    16861 gggatcggga aaaacagttt aatgaggtta tgcgtcgcat ctctgaaatg aaagatgaga
    16921 agcaagcggc gagcctcgct gaccagctca tggggggcga ggctaataag atcatgacgt
    16981 atatgaaggc gacgggtaaa agctgggaac agacaatgtc tgacgcgcaa aagtcgaatt
    17041 tgttaacaaa agagggtgcg gaaggggcgg cgcgtgcgca tgtatcagtg acaaatctgt
    17101 ggggctctat tacgtcggga cttgcagata cactcggtaa aatcgggggg gaactcgcgc
    17161 cgacgtttga ctcagtacgt gaaacattta catcctggtt taaggataat cagggtggtt
    17221 ttgtcagcac aattactgag tgggtaaaac cagaaaatat gaaaaaaatg tgggatggca
    17281 tcgtcaattt tggagaaggt tgcgtcaaat tcgggaagat tatatgggct gtcgtaaaga
    17341 aactggagtg gcttattccg gatgaaaaat cagatgaaga acagaaaact tataatgacg
    17401 aatataatcg ggcatatcaa gagtttatgg attcgggagg taaatattca ccgaacgctg
    17461 gattgactgc tgataatatt gcgaaggcaa aagccgaaga ggctgtcgaa aatatgcggc
    17521 atccagaacg gcaagaacag gcaaaatctc aggcagaatc aatgctcgcg tttgttaatc
    17581 cattatccgg gatggtaaat aaaaataccg cgcctgcagc actggattcg tcgacgttaa
    17641 atattgatac gttgaagcag gcagtctcga cacctgcgcc ggagcaaaac aataaaatcg
    17701 aaatcaatat tgtcggtgcg acagatccgc agtcaataca acaatctgca gcgtcggggg
    17761 tgctcgatgg actgagacag gctgcgagtt cgtacaatcg tggcgcgatg tttgataaac
    17821 cagctgcggc ggggtgacaa tgagtgatgc aataaacggt gctgaggata tcatgctcgg
    17881 cctgggtgat ttcatttttg ctatatcaac agtagcgtac aacaaactgc aacgtagtga
    17941 cgcctggcgc tgggctcagc aaacacgatt cgggaaaaac gacgcgttgc agataaccgg
    18001 acgaccgaac cccacaatta ctatcgatgg aaagataaac gctttatttc ttgacgggtg
    18061 cggcgtcggc ctgttgacag atttgcgagc gctcgggaac acgggagaac cccagcagct
    18121 agtgctgggg accggggagg tgaaagggta ctgggtgctg cgagaactaa ctgaaacgca
    18181 aaacagcttt ttgatgggcg gaacacctaa atcgcaggac ttttcactca cgctcgaata
    18241 ttatggagct tcgctagatt aatacaaaat caag
//
LOCUS       DAOFSV010000086        18017 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.086, whole genome shotgun sequence.
ACCESSION   DAOFSV010000086 DAOFSV010000000
VERSION     DAOFSV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.086"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..710)
                     /locus_tag="QBH27_003070"
     CDS             complement(<1..710)
                     /locus_tag="QBH27_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDQ7015351.1"
                     /translation="MKSDLTIKNRYCTIPQAKFRKWDEMDVLLWKLGKNDSRRRSGVY
                     YLNAYKDAYVQYNRDKIIKYAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIG
                     LLDMPENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFQIAATYL
                     RDLVLFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFICLINNLPARDTDDYKFIS
                     YGMRLLEIREHIKKLINE"
     gene            complement(713..1192)
                     /locus_tag="QBH27_003071"
     CDS             complement(713..1192)
                     /locus_tag="QBH27_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDQ7015352.1"
                     /translation="MSNMIYLSIKGKIQGLISEGCGSYASIGNKYQINHVDEIFVLQF
                     DHSLSREYNVVHHPIKFYKPIDKSSPLLNAALSENEELSAVFNFYRINSGGCIERFYT
                     IELQGAHLSNILTCYPHSITHAGNQPEEVIVLNYKNITWKHHIASTESYSCWEERIF"
     gene            1443..2024
                     /gene="mdaB"
                     /locus_tag="QBH27_003072"
     CDS             1443..2024
                     /gene="mdaB"
                     /locus_tag="QBH27_003072"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDQ7015353.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            2055..2369
                     /locus_tag="QBH27_003073"
     CDS             2055..2369
                     /locus_tag="QBH27_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDQ7015354.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(2414..3301)
                     /locus_tag="QBH27_003074"
     CDS             complement(2414..3301)
                     /locus_tag="QBH27_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDQ7015355.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(3298..4245)
                     /locus_tag="QBH27_003075"
     CDS             complement(3298..4245)
                     /locus_tag="QBH27_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDQ7015356.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(4256..5293)
                     /locus_tag="QBH27_003076"
     CDS             complement(4256..5293)
                     /locus_tag="QBH27_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDQ7015357.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(5302..6285)
                     /locus_tag="QBH27_003077"
     CDS             complement(5302..6285)
                     /locus_tag="QBH27_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDQ7015358.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(6282..7091)
                     /locus_tag="QBH27_003078"
     CDS             complement(6282..7091)
                     /locus_tag="QBH27_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015359.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            7465..9606
                     /locus_tag="QBH27_003079"
     CDS             7465..9606
                     /locus_tag="QBH27_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDQ7015360.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(9670..11562)
                     /gene="parE"
                     /locus_tag="QBH27_003080"
     CDS             complement(9670..11562)
                     /gene="parE"
                     /locus_tag="QBH27_003080"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDQ7015361.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRLDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11591..12172)
                     /gene="yqiA"
                     /locus_tag="QBH27_003081"
     CDS             complement(11591..12172)
                     /gene="yqiA"
                     /locus_tag="QBH27_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDQ7015362.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(12172..12999)
                     /gene="cpdA"
                     /locus_tag="QBH27_003082"
     CDS             complement(12172..12999)
                     /gene="cpdA"
                     /locus_tag="QBH27_003082"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDQ7015363.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(13024..13446)
                     /locus_tag="QBH27_003083"
     CDS             complement(13024..13446)
                     /locus_tag="QBH27_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDQ7015364.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(13447..14076)
                     /gene="nudF"
                     /locus_tag="QBH27_003084"
     CDS             complement(13447..14076)
                     /gene="nudF"
                     /locus_tag="QBH27_003084"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDQ7015365.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            14281..15762
                     /gene="tolC"
                     /locus_tag="QBH27_003085"
     CDS             14281..15762
                     /gene="tolC"
                     /locus_tag="QBH27_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDQ7015366.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            15910..16581
                     /locus_tag="QBH27_003086"
     CDS             15910..16581
                     /locus_tag="QBH27_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDQ7015367.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            16587..17747
                     /locus_tag="QBH27_003087"
     CDS             16587..17747
                     /locus_tag="QBH27_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDQ7015368.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         4876 a         4689 c         4170 g         4282 t
ORIGIN      
        1 tactcattaa tcaatttttt gatatgttct ctaatctcta acaacctcat accataagag
       61 atgaatttat aatcatctgt atcacgcgca ggcaaattat taattaaaca aataaaaaaa
      121 cccaaatctc gttcaacacc acgattatat ctaataccag ccataatata ctgctcattc
      181 gtcatataca gtgttgccga agagggataa tcaaaaagaa caagatctct taaataagta
      241 gcagcaattt gaaaggtaaa atcatcctcc attaggcatg tcgctaactc taactgattt
      301 ctggtagtta attctgaagg gtctagacct agagtaatcg ctgcggttcg tatttgtata
      361 cccattgagc caaatgatgt tttattttca ggcatatcta acaatccgat cattcttttt
      421 gtttcatata tctgaaactt ataattttca ggcatacctc ctgactcaat ccaggccacg
      481 ccgcccagta actctggacg aattccagcg gcgtatgcat atttaattat tttgtctcta
      541 ttatattgta catatgcatc tttataagca tttaaataat atactcctga acggcgacgc
      601 gaatcatttt ttcctaactt ccagagtaaa acgtccattt catcccattt acggaacttc
      661 gcttgtggaa ttgtacaata tctatttttt atagtgagat cgcttttcat atttaaaaaa
      721 tcctttcttc ccaacaacta tatgattccg tactcgcgat atggtgcttc catgttatat
      781 ttttataatt aagaacaatg acctcttccg gctgattacc cgcatgtgta attgagtgtg
      841 gataacatgt cagaatattc gataaatgtg ctccttgcaa ttcaattgtg taaaaccttt
      901 caatacaacc gccagaatta attctataaa aattaaatac agctgataac tcttcatttt
      961 cagaaagagc ggcatttaat aaaggggagg atttatctat tggcttgtaa aattttatgg
     1021 ggtgatgcac tacattatac tctcgactta aagaatggtc gaactgtaaa acaaaaattt
     1081 catccacatg atttatttga tatttattac caatagatgc atacgaacca caaccctcag
     1141 agatcaaacc ttgtattttc cctttaattg ataagtatat catatttgac atatatattc
     1201 tccataatca ttttttccga aaaactcctg tatcatttgc ataatacatc tctcctgtgg
     1261 gagcttatgg gaagttgatt taagtattgt cattttcaga aaatatttat cccgcatcgg
     1321 tgaatcagag aaagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa
     1381 tttcaccgta ccgctctgtg acgtactata gtcggcaaac gtctcacctt gaggttaaaa
     1441 aaatgagcaa catcctgatt atcaacggcg cgaaaaaatt cgcccactcc aatggtcaac
     1501 tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg cgacctcggg catgatgtcc
     1561 gcattgttcg cgccgacagc gactacgatg tcaaagcgga agtacaaaac tttctctggg
     1621 ctgatgtggt gatctggcag atgcctggct ggtggatggg cgcgccgtgg acagtgaaaa
     1681 aatatattga tgatgtattc accgaaggtc acgggactct gtatgccagc gatggtcgta
     1741 cccgcaaaga tccatcaaaa aaatacggtt ccggcggcct ggtacagggc aaaaaatata
     1801 tgctttctct gacctggaac gcaccaatgg aagccttcac cgaaaaagat cagttcttcc
     1861 acggcgttgg tgttgacggt gtgtacctgc cgttccataa agcaaaccaa ttccttggca
     1921 tggaaccgct gccgacattt atcgctaatg acgtgataaa aatgcctgat gttccccgct
     1981 atactgaaga atatcgcaag catcttgtgg aaatttttgg ttaactagag ctctggcttt
     2041 agaaggagtt aaccatgatt acagtaatcg cagaaattcg tactcgtcct ggtcaacatc
     2101 accgtcaggc agtgttggat cagtttgcta aaatcgttcc aaccgtactg aaagaagaag
     2161 gttgccacgg ctatgcgcca atggtggatt gcgctgctgg cgtgagtttc cagtctatgg
     2221 caccggattc tatcgtgatg attgagcagt gggaaagcat cgcgcatctt gaagcgcatc
     2281 tgcaaacccc gcacatgaaa gcatatagcg aagccgtaaa aggtgacgtg ctggagatga
     2341 atattcgtat cctgcagccg gggctttgac atttctaaaa agaggccggt gctgctaccg
     2401 gtctcagccc agattagcca ttctttcttc ccgttttcag tcctcgatgg aaatcgttaa
     2461 ttcgcttaat ggttttaacc gcacgctgcg gcactgcgga agctaccgac cagacaatca
     2521 tttccagaca caacaatacc gtaccatgta gcggcgtttt ccctttttca tccccgcgcg
     2581 gaacatgaat cacgatactg gcgtctttgc taaaacgtga atccagcgcg tttgtcagca
     2641 aaataacagg gatccccagc cgcctcgcct cacgaagcgt tgtcagccct tcccggtgcg
     2701 cagatttttg cgccatcatg atcagaacat cgccacgttg aagcgcgatc agttgctcgg
     2761 caaggccaat acccgtacgg tttagtgccg tggcaggcaa acctatgcgg ttgaatagtc
     2821 gtgcggtata gtccgccaga atacccgaag cgccaatgcc aaaaattgcg acttgccgtg
     2881 cctgcaccaa caacgctaca gcttgcgcaa gcgcgtggcg gttttcaggc tctgacagga
     2941 cattacaggt atacaaatgc ccttcaagaa caaagtcgat tgcggtgtta acatcgctcg
     3001 tcaagttact caccgtcgta gacatctttt cgctggaact aagggctgga ccaagccact
     3061 gttccagagt gcgtttcaga tcacgcagcc cggcaaaccc cagggcctgt atagccctga
     3121 taactgtggc atcggaagtg ttcaacgtgg ccgcaatttc catcgccgtt tgctcgagta
     3181 ccacttcacg atgctcatta atgtagctgg cgacaactct taacccaggc gttaactgac
     3241 tcgcgcgagt gcgaaaacgc tcaccaaaaa tatcaacccg cactttatct ttctggatca
     3301 tttagcctcc ggtagaggtc gccctgcaat ttgtgattgt atctgcgcag ccatcagccc
     3361 cagagatgca aaggagttag aaattgcctc ttcacgagaa atcagcacat aacgcccgga
     3421 acgacaggca gtcagaaact gacaccatcc cggcatgact ttttccattg ctgccagctc
     3481 atcctgaggt ttaccaccgg tgtcaccgcg ccaggttgca aaaacaaaat cggcatccag
     3541 ttcaggtaaa cgctccgcgc tcacatccat tcgcccacct tcaggaatgc tctcaatcag
     3601 cggcggaaaa cgaaaaccag catcgcgtaa tacccgtccc agcgaatgat agctatgcat
     3661 cacattaatt tttccctggt ttgcctgaat caccgatacc gttatttttt ggctgtccag
     3721 cgtcgccttc agcgcattga tttgtgcctg atagcggcgt tcaagtatcg ccagctgcga
     3781 ctgcgttcct gtaagttctg ccagcttgcg atagatttct ggcgcgcccc ctttgagatg
     3841 atcaatgctc accgtgggcg caattttttc aagttgttca atgggcgtat tacgggtcgg
     3901 ttctgtgatg atcaagtcag gctttgccgc cacaatcgct tcaatatcaa tatcagcggt
     3961 accaataaag gcgatcgatg aattatcaaa gtcgacacct gtcaataacg cgccagatcg
     4021 aatgaaatgg ctaccgtcag gccgagttcg cccgtggctg gcgacaggag gtacaccgag
     4081 ctcaatcaga ggaatagtga tatcaagatc gtgtaaagag acaattctct ttggatgagc
     4141 aggaacagtg actttgcgcc ctaaatcatc agtaaatacc tgaacaggtt ctgttgcacg
     4201 ggaaaaaaat gacaacagta tcagcagaga aaaaaataaa cgcatataac tcccgttaaa
     4261 aactgttcct gcgctgccag agcagtagta aaaaaaatgg tccaccaatc agtgagataa
     4321 tgattccggc aggcagttgc aaaggcaaaa aagccaaacg accaatgcta tccgccagaa
     4381 taactaacaa cgcccccgat accgcgctcc ccagcagtaa tgtcgtctgc ccgccacgta
     4441 gaataaaacg tgatatatgg ggtgctatca atccgacaaa accgatgtta ccaacacacg
     4501 aaacgcagac ggctgtcagg attatgggtg cggcgacacg taacaacgcc agacgagaac
     4561 tattcacccc taacccggtt gcaacctgat gaccaagcag agcgatatcc gcagtcctgg
     4621 cggtgaacag caataaaaga gcggctggca acatccaaca ggctgaaata ccgactaaca
     4681 tccagttagc tgcatgcagg cttcccgata accacatcaa tgcagtttgt acatctcgaa
     4741 catctgcagt ggtcataaat acaccgatac cggcggcgaa aaaccaggaa acaccaatac
     4801 caatcagaac aaatcgcggg cgggaaatat cgcgcgcaca aaaaatcacg attaaagcca
     4861 ccaacaggcc accagcaaga ccagccactg gacgccagaa cattcccagc attggaaact
     4921 ggaaaatcag ccacagcaca gccacgctac atccttcttt gacgccgatt aaaccaggat
     4981 cggcaaggcc gttacgtgca atggattgca ttgcggcccc tgccatacct aacatggccc
     5041 cgcatagcaa tgccatgatg actctcggca agcgaatatc ctgcacaata taacgtgcgt
     5101 ctgtgcttac gttttctgga gcaaacaacg ctcgtcctac ttccgatgcc ggaacagaga
     5161 aagaaccctg catcagaccg aatatcagta ttccaaccgc aagcaggaac agcccaccaa
     5221 gcaacagcag atttttaggg cgcaccagtg tcgaaaactt accaacacgt agtgggcgta
     5281 gcccgacgtt cattatccga ctcatttgaa catcctcgtc gccataatga caaaaacagg
     5341 cgcaccaact aacgccgtca tgacaccggt cgctaactca tggggcgtga aaagcgtccg
     5401 ggcaataata tctgccagta ataacaaaag cgcgccgaca caagctgaaa gagggagcag
     5461 aacgcgcaga tccgctgata ccagacgccg gacaatctgc ggtactagca ggccgacaaa
     5521 accaatgggg ccagcaatcg atacggctgc gccacagagc aacgcgatcg caagcagggt
     5581 gaatgttcgc gtgcggagta cagagacgcc cagcccctgc gccatgcgat cgcccagtgc
     5641 caacatgtta agggagggtg caaggtaaag ggctaacaca aatcccccca gagaaaacca
     5701 ggcggacgta cccagcgtcg cccaatcctg ccccgctaaa tcacctgcta gccatgtccg
     5761 catcgccagc agtgtttgtt cgtcaagaat caggattgca gccgttattg aggacacgaa
     5821 cgccgatagc gccacgccgc ataaagtaac tttcattggc gttaaccctg aacgccctgc
     5881 ggaggaaagc agcaatatca acaggaacaa taacgcgccc cccgtagatg ccagcagtgg
     5941 gcgtccaacc ggaaaggcca gtccaagcgc actggcggca acgacagcaa gagcggctcc
     6001 ggcatttaag cctaaaatat gtggttcccc caatgggtta cgaatgacag cctgcaacaa
     6061 agcaccagca acaccaagag aagcaccggt aagcaatgct gccgctaaac gcggcaaacg
     6121 gagtcttaca attacgttgt gatcgaagtt ttgcggatca aaatgaagaa atgcctgcaa
     6181 caccgtttgt ggtgcaataa atcgggcacc aatccctgga tataccacgc atcccaccag
     6241 cagtaaggcg aggaaaatta cagtaaccag ccctgtgcgc atcatacttt ttctacacca
     6301 cgaaacggca tgaagaacgg ctttcccgtc agaggattta ttgatgcgtg aacatcaacg
     6361 tcaaagacag cttttaccag ttcaggcgtg caatgctccc cctcattcaa cacacgcacc
     6421 actttcccct ggcgtaaaaa tatcaacgta tcgccataat tgacggcaaa attcagatca
     6481 tgcaacacca cgacgactgt acgcccgtga tgacgcgtta aatcatgtaa taactcaaga
     6541 atttcaacct gataacgtaa atcaagccag gttgtgggtt catccagcag gatataaggc
     6601 gtcttttgcg ccaacaccat cgcaatccag caacgctggc gctgcccacc agagagtttt
     6661 tcaacaggta aatgtgcaaa ctcctgcgta ccggttaatt tcagggcctc ttccaccgcc
     6721 tgctcatcgg cgtcgctcca ttgacgtatg aaattttgcc agggaaaacg cccacgcgac
     6781 accagttcat aaacggttaa gccctccggc aataatggcg actgcggcaa gatcccaagt
     6841 ctacgggata acgctttcgt tggttgctcg tgaatcgctt tgccatcaag caatatcgaa
     6901 ccgcccatag gttgcaagat acgtgcaatg gtgctgagca acgtcgactt tccacagccg
     6961 tttgccccaa ccagaacggt cattttttct gtgggaacgg agaaagaaac accatcaaca
     7021 atgattttct tgtgataccc ggcagaaaca ttgtcgagga tcagcccctg cccttttgcg
     7081 ttatgtgcca cgtgtacgcc aacttaatca aagacaaaca aaaataaaac tcattctcct
     7141 ttgagagttt gacgccacaa aatgtagtag tcaagcagtt aaaagccaca caaaaacaag
     7201 gtaaataaca ggtaaaatat ttttatacat aaaaataaaa acattaaata tcaataaatt
     7261 aaaagcaatc aaattgtata aacctttcat aaaaacacat gtagcaattt tttgttattt
     7321 ctcatttact actacattac tgcgtgattg aatgattctc aattacatat ccgatggcga
     7381 aataacaaac ggatagtgct tagtgagcaa taagaaacaa ttttcactcg cgacaatccg
     7441 cgagtttttg ggataacaga ctaaatggct atgttcacac cttcattctc aggactcaaa
     7501 ggtcgggcgc tcttttcact gctttttgcg gcaccgatga ttcatgcaac agactctgta
     7561 acgaccaaag atggcgaaac aatcactgtt acagcagatg caaataccgc aactgaggca
     7621 accgatggtt atcaacctct gagcacctct acggcgacat taaccgatat gccgatgctg
     7681 gatatcccgc aggtggtcaa tacggttagc gatcaggttc tggaaaacca gaatgcgaca
     7741 acgctggatg aggcgcttta taacgtcagt aacgtggtac agaccaatac attaggcgga
     7801 actcaggacg cctttgtacg ccgtgggttt ggcgcaaacc gggatggctc catcatgacc
     7861 aacggtctgc gaaccgtact tcctcgcagt ttcaacgccg ccacagaacg tgtggaagtg
     7921 ctaaaaggcc cggcctccac gctgtatggc attctcgatc ctggcggact gattaacgtc
     7981 gtgaccaagc gcccggaaaa aacattccat ggttcggttt cagccacctc ctccagtttt
     8041 ggtggcggca ctgggcaact tgatatcaca ggtcccattg aaggcactca gctggcgtat
     8101 cgccttaccg gggaagtgca ggatgaagat tactggcgaa acttcggtaa agagcgcagt
     8161 acatttattg ccccgtcact cacatggttt ggtgataatg caacagtaac catgctctat
     8221 tcccatcggg actataaaac tccgttcgat cgtggaacga ttttcgacct tacgacgaaa
     8281 cagcccgtaa acgttgatcg aaaaatacgt tttgacgaac cgtttaatat tacagatggt
     8341 cagtccgatc tggcgcaact caacgcagaa tatcatctca atagccagtg gacagcacgc
     8401 tttgattaca gctacagcca ggataaatac agcgataatc aggcgcgtgt taccgcatat
     8461 gatgcaacga caggaacact gacacggcgt gttgatgcaa ctcagggctc tacccagcgt
     8521 atgcatgcta ctcgtgcgga tctgcaaggg aatgttgata ttgccggatt ctataatgag
     8581 attctgggtg gggtgtcata tgaatattat gatcttctgc gtacagatat gattcgctgt
     8641 aaaaaagcta aagatttcaa tatttacaac cctgtttatg gtaataccag caaatgtaca
     8701 acggtttcgg cgtcggacag tgatcagacg atcaaacagg agagctactc agcgtatgca
     8761 caggacgcac tctatctgac cgataactgg attgccgtcg ccgggatccg ctatcagtat
     8821 tacacgcagt atgcgggtaa aggccgtcct tttaatgtca atactgacag ccgcgatgaa
     8881 caatggactc ccaaactggg gttggtctac aaactgacgc catcggtatc cttatttgcc
     8941 aattattcgc aaacatttat gccgcagtcg tcaattgcca gctacattgg cgatcttcca
     9001 ccggaatcat ctaatgctta cgaagtcggg gcaaaattcg agctattcga tggtatcacc
     9061 gcagatattg cgctgtttga tatccataaa cgtaatgtgt tgtataccga aagtattggt
     9121 gatgaaacca tcgccaaaac ggcaggccgt gttcgttcaa gaggggtaga agtcgacctt
     9181 gcgggagcat taactgaaaa cattaatatc attgccagct acggctatac cgatgctaag
     9241 gttctggagg atcctgatta tgcagggaaa ccattgccga atgttcctcg tcataccggt
     9301 tcgctattcc tgacctatga tattcataac atgccaggca ataatacgct gacatttggc
     9361 ggtggtggac atggtgtaag ccgtcgttcg gcaaccaatg gggctgacta ttatctgcca
     9421 ggctattttg ttgccgatgc cttcgccgca tacaaaatga aattgcagta tccggtcaca
     9481 ctgcaattaa acgtcaaaaa cctgtttgat aaaacgtatt acacctcttc catcgccaca
     9541 aataatctgg ggaatcagat tggcgatccg cgtgaagtgc aattcacggt gaaaatggaa
     9601 ttttgatcaa aaaagcccgg tatacacttg tataccgggc tatgttttat tcttctggcg
     9661 ggaggattat cagacctcaa tctctgccat gtcgcctttt tcttgcaacc agttgcggcg
     9721 atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc
     9781 ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcgcg
     9841 caattgcatc gggttcattt ccccgagacc tttaaaacgc tggacgtttg gcttgccttt
     9901 cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac
     9961 ctctttcccg aggtcaagac ggtagagcgg tggcagtgca acgtaaacgt gaccgtgttt
    10021 caccaatgcg cggaagtgtt ttacaaacaa agcgcagagc agcgtggcaa tgtgcagacc
    10081 atcggagtca gcatccgcca ggatacagat cttgccgtaa cgaagctggc tcaaatcgtc
    10141 gctgtcagga tcgataccga tagctaccga aatatcgtgc acttcctgcg aagccagcac
    10201 ttcgtcggaa gagacttccc aggtgttaag gatcttacct ttcagtggca tgatcgcctg
    10261 atattcgcga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacaaggaa
    10321 tagttcggta cggttaagat cctgcgcggt acagtcagcc aatttaccag gcagcgccgg
    10381 gccgctggtt agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct
    10441 ggaaatcgcc atctctgcca acagctccgc cgcctgaacg ttttggttca gccacaggat
    10501 gaaggcatct ttcaccacgc ccgaaacgaa tgccgcacac tgacgcgaag agagacgttc
    10561 tttggtctgc ccggcaaact gcggatcctg catttttact gacagcacat aggcgcagcg
    10621 atcccagata tcttccgccg acagctttac accacgcggc agaatgttgc ggtattcaca
    10681 gaactcacgc atcgcgtcca gcaggccctg acgcaggcca ttaacatggg taccgccctg
    10741 catcgttggg atcaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca
    10801 cagtagcgcc cagtccaccg cttcagtatc gccagcgaaa ttaccgataa acggtttttc
    10861 tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata
    10921 gcaccagcgc tgttcggtgt tgttgatctc atctttaaaa gtgatctcaa cgcccgggca
    10981 cagtaccgct ttggctttca gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc
    11041 aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt taccgcaagt
    11101 gccgacaacc tgtaaatctt gcaccttttc gccattttca aaggcgatgt tatagatctg
    11161 accatcgcgg cgtacgttaa cttctacgcg cttcgacagg gcgttaacta ccgaaatccc
    11221 cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac cgcccgcgtg
    11281 cagacggcaa agaatcagtt caaccgccgg taccccctct tccgggtgaa tatctaccgg
    11341 catcccgcgc ccatcgtcaa tcacttctaa cgactggtcg gcatgtaaga ttacatccac
    11401 gcgttttgcg tggcccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa
    11461 atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc
    11521 ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc
    11581 gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt
    11641 cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt
    11701 acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca
    11761 aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact
    11821 ctagcacata ttgctgcccg gtgtaggggt tctcgttctg accgagatag tccgtcagca
    11881 gttcaaacgg gcgcaccgcc gggttgacca ccactgcggg cagcataaaa cattgcgaca
    11941 accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat
    12001 gttccaggac aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcagctgcg
    12061 gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt
    12121 ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc
    12181 ttctgaagcg gtatcaggtt ggaagcgtgc gtccgccagg cgatgcacct cggtggtcag
    12241 cgtgccatca gcatgtaact caagagtacg ccagccgggc gcgatggtat ccagcgtaaa
    12301 gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc
    12361 gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg
    12421 aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc aggctgtgtt gatcaagcca
    12481 actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc
    12541 atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac
    12601 gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt
    12661 cgccggggag atacccgcat cctgtaacgc actgtacatc gcgggctgga aatcgtggtt
    12721 gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata
    12781 ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg
    12841 ctggtgtggc cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa
    12901 cagggcttcg tgcttttgtg caaacaggtg agtgtcggta atttgtaaaa tcctgactct
    12961 ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg
    13021 acgctaataa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc
    13081 taaaaactga ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc
    13141 ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc
    13201 atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg
    13261 ttctattgtc accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc
    13321 gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg
    13381 caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg
    13441 cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc
    13501 gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg
    13561 gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccgtgaa taccgcttgc
    13621 ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct
    13681 tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc
    13741 ttcgcgacgg gcaacatctt ccacactttc accctcttca atcatcccgg caaccatctc
    13801 cagtagccaa ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct caatcagcac
    13861 aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat
    13921 ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag
    13981 atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt
    14041 gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctgg cactaatagt
    14101 gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcatg tgttaaattg
    14161 aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag
    14221 aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa
    14281 atgaagaaat tgctccccat tcttatcggc ctgagcctct ctgggttcag ttcgttgagc
    14341 caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt
    14401 aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta
    14461 ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac
    14521 ggcatcaact ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg
    14581 tcgaaatggc gtgcgttaac gctgcaggaa aaagcagctg ggattcagga cgtcacgtat
    14641 cagaccgatc aacaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat
    14701 gcgattgacg ttctttccta tacacaggcg caaaaagaag cgatctaccg tcaattagat
    14761 caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc
    14821 gcacagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg
    14881 gtagagcagc tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc
    14941 gaaaacttta aaaccgacaa accacagccg gttaacacgc tgctgaaaga agccgaaaaa
    15001 cgcaacctgt ccctgttaca ggcgcgtttg agccaggacc tggcgcgcga gcaaattcgc
    15061 caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac
    15121 acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga cgatagcaat
    15181 atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt
    15241 aactcgcagg tgaaacaggc ccagtacaac tttgttggtg ccagcgagca actggaaagc
    15301 gcgcatcgta gcgtcgtaca aaccgtacgt tcctccttca acaacattaa tgcttctatc
    15361 agtagcatta acgcctacaa acaagccgta gtttccgctc aaagctcatt agacgcgatg
    15421 gaggcgggtt actcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg
    15481 ctgtacaacg ctaagcaaga gctggcaaat gcgcgttata actacctgat taaccagttg
    15541 aatattaagt cagctctggg tacgttgaac gagcaggatc tgctggcact gaacaatgcg
    15601 ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc ggaacagaat
    15661 gctattgctg atggttatgc gcctgatagc ccggcaccag tcgttcagca aacatccgca
    15721 cgcactacca ccagtaacgg tcataaccct ttccgtaact gacgacgacg acggggcttc
    15781 ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct
    15841 tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg
    15901 aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc
    15961 gcacgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct ggcgggctgt
    16021 gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac
    16081 ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt
    16141 actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc
    16201 cagcaggcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc
    16261 agcgggagtt tctggatgcc gctgatggcc ggttacatga tgggacgtct gatgggcggc
    16321 ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccggccag cccggcttac
    16381 ggtaaatata ccgacgcgac cggtaaaaac tatggcgcag cccagccagg ccgcaccatg
    16441 accgtaccga agacggcaat ggcaccaaaa ccggcgacca ctactactgt tacccgtggc
    16501 ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct
    16561 tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact
    16621 ggcgcgaaaa agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact
    16681 ggtgtgaaga tgcttactac aagttgaccc tctctcaggt tgaaaagctg gaagaagtca
    16741 ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc
    16801 tgatgaccaa attccgcata ccaaaacaca cctggagttt tgtgcgccag tcatggctga
    16861 cgcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact ggcgaaccta
    16921 aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg ttctttcagt
    16981 ggatctggct ggaagatcag cttaacgccg gtaacttgcc ggagggcagc gaccagttta
    17041 acagtctgca agaaaagctg atcgatcgct tcgttgagct gcgtgaacag tatggcttcc
    17101 agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt
    17161 tgcaggactg cgcaacggaa gctgaaatcg ccaccgagtt cctctacatt gacgatatcg
    17221 ggttaggtga aaaaggtcag ttcacggatt tacaggatca ggtgatttcc aacctgttca
    17281 agctgtatcc gtgggaattt atgttgcgtg agatgttctc caccaagctg gaggatgcag
    17341 gcgtacgctg gctggaaccg gcgtggaaga gcattatctc caacaaagcg cttctaccgc
    17401 tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt gcggaagatg
    17461 atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg
    17521 tgtcgatcat tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag
    17581 ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg
    17641 gtagctggct ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcattga
    17701 tcacccagga tatgtctcgg ttttatccac atatttttgt tgagtaatac agcttaatat
    17761 tgccccgata agcggggcaa tctcttataa tccaccattc ataattccca tcaatatata
    17821 aacacataca tatccaacct ttatttaaca ttaatactca atttaatgtt aaatcgtcat
    17881 atgcaagcga tattgtaatt aaagtaagtg aatacatcac aacataacta tttgatatta
    17941 tttaaatact ttacttttat ctcaccagat ttaaatttaa taattaaaaa acaatgttgc
    18001 agggatcaca ctacaca
//
LOCUS       DAOFSV010000087        17680 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.087, whole genome shotgun sequence.
ACCESSION   DAOFSV010000087 DAOFSV010000000
VERSION     DAOFSV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.087"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            99..383
                     /gene="traQ"
                     /locus_tag="QBH27_003088"
     CDS             99..383
                     /gene="traQ"
                     /locus_tag="QBH27_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDQ7015369.1"
                     /translation="MISKCRFSLPRLDITGMWVFALGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            370..915
                     /gene="trbB"
                     /locus_tag="QBH27_003089"
     CDS             370..915
                     /gene="trbB"
                     /locus_tag="QBH27_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDQ7015370.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            845..1192
                     /gene="trbJ"
                     /locus_tag="QBH27_003090"
     CDS             845..1192
                     /gene="trbJ"
                     /locus_tag="QBH27_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDQ7015371.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            1140..1565
                     /gene="trbF"
                     /locus_tag="QBH27_003091"
     CDS             1140..1565
                     /gene="trbF"
                     /locus_tag="QBH27_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDQ7015372.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            1552..2925
                     /locus_tag="QBH27_003092"
     CDS             1552..2925
                     /locus_tag="QBH27_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDQ7015373.1"
                     /translation="MLRIKPLLVLCAALLTFTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMSYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            2922..5741
                     /gene="traG"
                     /locus_tag="QBH27_003093"
     CDS             2922..5741
                     /gene="traG"
                     /locus_tag="QBH27_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDQ7015374.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAMESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASNDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSV
                     QERMPGADSPQELMKRAKEYQDKYK"
     gene            5760..6257
                     /locus_tag="QBH27_003094"
     CDS             5760..6257
                     /locus_tag="QBH27_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HDQ7015375.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLVGWGKAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKNQYDGSEKLDFGGIEQPPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            6289..7020
                     /locus_tag="QBH27_003095"
     CDS             6289..7020
                     /locus_tag="QBH27_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDQ7015376.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPDSERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            7223..8002
                     /locus_tag="QBH27_003096"
     CDS             7223..8002
                     /locus_tag="QBH27_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015377.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKKLTEILGKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVLCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSHIILFCPVPDFPGVSS"
     gene            7999..10215
                     /gene="traD"
                     /locus_tag="QBH27_003097"
     CDS             7999..10215
                     /gene="traD"
                     /locus_tag="QBH27_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDQ7015378.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQ
                     LPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            10215..15485
                     /gene="traI"
                     /locus_tag="QBH27_003098"
     CDS             10215..15485
                     /gene="traI"
                     /locus_tag="QBH27_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDQ7015379.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFPPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTEAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDEPRTAEKLARHPSFTVVSDQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGTSETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGRGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRTASSERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            15505..16251
                     /gene="traX"
                     /locus_tag="QBH27_003099"
     CDS             15505..16251
                     /gene="traX"
                     /locus_tag="QBH27_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDQ7015380.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            16306..16866
                     /locus_tag="QBH27_003100"
     CDS             16306..16866
                     /locus_tag="QBH27_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDQ7015381.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            16997..17209
                     /locus_tag="QBH27_003101"
     CDS             16997..17209
                     /locus_tag="QBH27_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015382.1"
                     /translation="MNGFRNSSRNGQVWCWQRAGSRAVILEVSGRRMEAAEAWRRAAG
                     VAPRTDWQQFARKRAAHCHRRCRGRG"
BASE COUNT         4322 a         4226 c         5196 g         3936 t
ORIGIN      
        1 aaaccataca aaataatgag gttgatctga ttaatatcac acaaacgcga atattacaga
       61 catgaatatt ctcatgtaaa taataaccgg agtttaacat gataagtaaa tgcagattct
      121 ctttaccccg gcttgatatt acgggaatgt gggtatttgc tctgggtgtc tggtttcata
      181 tcgtcgcccg tcttgtttac agcaaaccct ggatggcttt ttttctggcg gagttgattg
      241 cggccatcct tgtgctgttc ggcgcatacc aggtgctgga tgcatggatt gcacgtgtca
      301 gcctggaaga gcgtgaggca ctggaagcca ggcaacaggc catgatggaa gggcagcagg
      361 agggcggaca tgtctctcac taaattactg ccggtcattt tgttgatgat ggccacaggt
      421 gtgcaggcct ccacccggga tgaaattgaa cgactctgga atccgcaggg tatggccgtg
      481 cagcctgcac aaccggcagc agacacatca gccagaacag aaaagccggc tccccgctgg
      541 ttccgcctca gcaacggcag gcaggttaac ctggccgact ggaaggtggt gctgtttatg
      601 caggggcatt gcccttactg tcaccagttt gacccggtac tgaaacagct ggcacagcag
      661 tacggctttt ctgtttttcc ctacaccctg gatggtcagg gtgataccgc ctttccggaa
      721 gcattaccgg tgccaccgga cgtgatgcag accttcttcc cgaatatccc ggtggccaca
      781 ccgaccacct tcctggtcaa cgtcaacaca cttgaggcat taccgctttt gcagggggca
      841 acggatgccg ccagttttat ggcgcggatg gataccgttc tgcagatata cggagaggaa
      901 aaaggtgcga aataagcagg tggtgttact cattgccgga atatccggca tcgcgacggg
      961 aataatcgtc agcctgaata tcccctttat ccgtcagggg cttttttatc ccgccagccc
     1021 tgtggaaatt gtcgtttcgc tgtgtcttac cttttctgtt tctgttgtgt tttttgtggg
     1081 ggcaattgtg ggatggattt cggtgtctga aatatattac agccgcatga cctgtctgca
     1141 tgaatcatca gaaatatcag agggaactta tgatgaaaga aaaaataaat gatacagaac
     1201 cgggaatcaa acaaattgaa agggagattg aacgtggttg tgataatgca aaaaaatact
     1261 tctggctttt tgtggttttc tttgctgccg gattgattgt aaggaacgtt atgcatgatt
     1321 ttttcagtgc cggtattgat tcgtggaaag ctgacccgga attaaataat tttcgttata
     1381 tgtggaacac cctgatgtat gtcattccga ttatgttata tgcgcttgca gctggttttc
     1441 ttgctgcggc ttctctgtcg ccgttgtgcg aaataatatt tgggggtgtc cggatattcc
     1501 tgctgaaacg ccgtatgcgc cgggagaaca cattaaggga gggcagcaat aatgcttcgc
     1561 attaaacctc ttctggttct ttgtgccgct ttactgacgt tcacacccgc agcgtcagcg
     1621 gatgtgaaca gcgatatgaa tcagttcttt aacaagctgg gcttcgcctc caataccaca
     1681 cagccgggcg tctggcaggg gcaggccgcc gggtatgcct atggtggctc cctgtatgcc
     1741 cgcacccagg tcaaaaatat ccagctgatt tccatgacgc tgccggatat caatgccgga
     1801 tgtggcggca tcgacgccta tctcggctcg ttcagtttta ttaatggcga tcagttgcag
     1861 cgttttgtta agcagattat gagcaatgct gccggttact tttttgacct tgccctgcag
     1921 acaacggtgc cggaaataaa aaccgcaaaa gacttcctgc agaaaatggc aagtgacatt
     1981 aacagtatga acctcagttc ctgtcaggtg gcacaggggc ttgtgggggg gctgttccct
     2041 cagacgcagg aatctcagca gaaagtttgt caggacattg gcagtgaaag caatatcttc
     2101 gccgactggg ccgcatctcg tcaggggtgc acggtcggag ggcagtcctc atctgttcgt
     2161 gacaaagcca gcgacaagga caaggagcgg gtgctgaaaa accagaatat catatgggag
     2221 gcgctgaaaa aaaacagaat gtttgacggc aacaaagagt tgaaagaatt tatcatgacg
     2281 ctcaccggta cgctgatttt tggggaagac agcgagatta cccccctgcc ggcgcgtacc
     2341 actgaccgcg atatcctccg ggcgattatg gaggggggaa cggcaaaaat ttaccactgc
     2401 aacgattcgg acaagtgtct gaaggtggtg gcggatgcaa atgtgaccat cgcccgggat
     2461 aaagccctga agtcacagat tacaaaactg ctcaccagta ttcagaataa agcagtgagt
     2521 gacactccgc ttgacgcccg ggaacagggg tttatttcca gcaccaccat tcccgttttt
     2581 aaatatctga ttgacccgca gatgctcggt gtgtccaaca gcgtcgttta ccagctgacg
     2641 gactatatcg gctacgacat catgctccag tacattcagg aactgcttca gcaggcccgg
     2701 gccatgattg ccaccgggaa ctatccgcag gctgtgatgg acaatgttct ggagaatctg
     2761 aatcaggcac agcaacagat tgccattttc cagagccagg tacaggtcca gcaggatgcg
     2821 ctgctggttg tcgaacgtca gatgagctac atgcgccagc agctttccgc ccgtatgctc
     2881 agccgttacc agaacaacta tcacttcgga gggaattcac tgtgaatgaa gtttatgtga
     2941 ttgccggtgg tgagtggttg cggaataacc tgaacgccat tgcggcattt atgagtacgc
     3001 ggacctggga ttccatcgaa aaaattgcgc tcacattgtc tgttctcgcg gtggccgtga
     3061 tgtgggtaca gcggcacaac gtgatggatt tgctgggctg ggtggccgtg tttgtgctta
     3121 tcagcctgct ggttaatgtc cgtacatcgg tgcagattat tgataacagt gacctggtca
     3181 aagtccatcg ggtggataat gtgccggtcg gtctggcgat gccactttca ctgacgacac
     3241 gtatcgggca tgcaatggtg gtcagctacg agatgatatt cacccaaccg gacagcgtca
     3301 cctacagcaa gacaggcatg ctgttcgggg cgaatctgat tgtgaaaagc accgatttcc
     3361 tgtcccggaa tccggaaatc atcaatctgt tccaggacta cgtccagaac tgcgtgctgg
     3421 gtgatattta cctgaatcat aaatacacgc tggaagatct gatggcctcc gctgacccct
     3481 acacgctgat tttttcccga cccagcccgc tacggggcgt ttatgacaac aataataatt
     3541 tcataacctg taaggatgcg tcggtcacgc tgaaagacag gctgaatctc gatacaaaga
     3601 cgggaggcaa gacctggcat tattatgtgc agcagatatt tggcggcaga ccggacccgg
     3661 acctgttatt cagacaactg gtcagtgaca gttacagtta tttctacggc tccagtcagt
     3721 ctgccagcca gattatgcgc cagaacgtca caataaatgc cctgaaagag ggtatcacca
     3781 gtaatgccgc ccgtaatggt gacaccgcca gcctggtgaa tctggccacc acgtcatcga
     3841 tggagaagca acgtctggcg catgtctcta tcggtcatgt gacaatgcga aacctgccaa
     3901 tggtacagac tatcctgacg ggaattgcca ttggtatttt cccgcttctg gtactggcag
     3961 ctgtcttcaa caaactgaca ctgtccgtcc tgaaaggcta tgtgtttgcc ctgatgtggc
     4021 tgcagacctg gccgttgttg tatgccattc tgaacagtgc catgacattc tatgcgaaga
     4081 tgaatggtgc gccggtcgtg ctgtctgaac tctctcagat acagctgaaa tactctgacc
     4141 tggcctctac tgccgggtat ctttcagcca tgatcccccc gttgtcatgg atgatggtaa
     4201 aaggccttgg cgcaggtttt tccagcgtgt acagccactt cgcctcttcc tctatcagtc
     4261 cgacggccag tgcagcaggc agtgtggttg acggtaatta ctcctacggc aacatgcaga
     4321 cggaaaacgt gaacggcttc agctggagca ccaacagcac cacgtcgttt ggtcagatga
     4381 tgtaccagac cggcagtggc gcaactgcca cacagacccg tgacggtaat atggtgatgg
     4441 atgcaagcgg agcgatgtcc cggttaccgg ttggtatcaa tgcaacgcgt cagattgcag
     4501 cggcacaaca ggaaatggcc cgggaggcgt cgaacagagc agaaagtgcc ctgcatggtt
     4561 tcagcagcag tattgccagt gcctggaaca cgctcagtca gtttggttct aaccggggga
     4621 gcagtgattc tgtcaccggt ggcgctgaca gcaccatgag tgcacaggat tccatgatgg
     4681 ccagccgtat gcgcagtgca atggaaagct atgcgaaggc gcataatatc agtaatgagc
     4741 aggcgactca ggaactggca tccagaagta cgagagcttc tgccgggatg tatggcgatg
     4801 ctcatgctga atggggggtt aaacctaaaa ttcttggtgt tggtggagga ttaggcgtta
     4861 gaggcggtgg tcgggctggc attgactgga gtgatgatga tgcgcatcag gccagcagtg
     4921 gttcacgggc cagcaatgat gctcgtcatg atatcgatgc cagagctaca caagacttta
     4981 aagaggccag cgattacttt accagtcgca aggtcagtga gtccggcagc catactgaca
     5041 ataatgctga ttcccgtgtg gatcagttgt ctgcagccct gaattcagcg aagcagagtt
     5101 acgaccagta cacgacgaat atgactcgca gccatgaata tgctgaaatg gcatcccgta
     5161 ctgaaagcat gagtgggcag atgagtgaag acttgtcgca acagtttgca cagtatgtaa
     5221 tgaaacacgc gccacaggat gcagaagcta ttctgacaaa taccagttca ccggagattg
     5281 cagaacgccg tcgggcgatg gcctggtctt ttgtgcagga acagatacag cctggtgttg
     5341 ataatgcctg gcgggaatcc cgtggcgata ttggtaaagg aatggagagc gtaccttcgg
     5401 gtggtggcag tcaggatatt attgctgacc atcagggaca tcaggccatt attgagcaaa
     5461 gaacgcagga cagtaatatc cgtaatgatg taaaacatca ggttgataat atggtcacag
     5521 aatataaagg gaatataggt gatactcaga acagcatacg tggcgaggaa aatattgtaa
     5581 gaggacagta ttctgaacta cagaatcacc ataaaacaga ggcattatct cagaataata
     5641 aatataatga agaaaagtcg gttcaggaaa gaatgcccgg ggctgacagt ccacaggaac
     5701 tgatgaaaag ggcaaaggag tatcaggata agtataagta ataatgctgt gtttgggaga
     5761 tgataatgag atgtctagcc catattgctc tggtaacggt gatacagttt atcgcctgct
     5821 atctggttgg ttgggggaag gccgaaacag tttttatgct gttcttcata gtgctatggc
     5881 agggactgtt tatatggttg ttctctcaaa tacgaaaaaa acggaatgtg tcggatgagt
     5941 ttaaattcag taaaggcgtt tggtatataa cgatgccagt atcttcctta ctctctccgt
     6001 tattaagcct gatggttttt atcattggta cactatacga attaaggcga gttagtggtt
     6061 gcataagcat aaaaaaatgg ggtcaaaatc aactcaaaaa ccaatatgat gggagtgaaa
     6121 agttagactt cgggggcatt gaacagcccc caacgaccta ctataaccct tcaaccggat
     6181 atcctatgca tggaggcttt gactcagcgg gaaatacttt tgggactcgt tggcaggatt
     6241 attatgacag gcaataagaa aactgtagtt tgtaaaaaag gtaatttgat gaaaacaaaa
     6301 aaattaatga tggttgcact ggtcagttcc actctggccc tttcagggtg tggtgcgatg
     6361 agcacagcaa tcaagaagcg taaccttgag gtgaagactc agatgagtga gaccatctgg
     6421 cttaaacccg acagcgaacg cacggtattt ctgcagatca aaaacacgtc tgataaagac
     6481 atgagtgggc tgcagggcaa aattgctgat gctgtgaaag caaaaggata tcaggtggtg
     6541 acttctccgg ataaagccta ctactggatt caggcgaatg tgctgaaggc tgataagatg
     6601 gatctgcggg agtctcaggg atggctgaac cgtggttatg aaggcgcagc tgttggtgca
     6661 gcgttaggtg ccggtattac cggctataac tcaaattctg ccggtgccac actcggtgtg
     6721 ggccttgctg ctggtctggt gggtatggct gcagatgcga tggtggaaga tgtgaactat
     6781 accatgatca cggatgtgca gattgcagag cgtactaagg caacggtgac aacggataat
     6841 gttgccgccc tgcgtcaggg cacatccggt gcgaaaattc agaccagtac tgaaacgggt
     6901 aaccagcata aataccagac ccgtgtggtc tcaaatgcga acaaggttaa cctgaaattt
     6961 gaagaggcga agcctgttct cgaagaccag ctggccaaat caatcgcaaa tattctctga
     7021 ttatcttccg gaggctggtc tgaccggcct cctgactgac gcctcgcttt gctcgttgtc
     7081 caaccccgga aagaaataaa ataaatttta tttctccccg gcgtttccga aataaatatg
     7141 aaaacactga gcggttgtgc aaattctttg tgcgaccgtg aagtgttgcc ggcgaagccg
     7201 ggatcttccg gagaaagtca ttatgacatc agacaatatc gctgaatatg ttggtgagaa
     7261 taatcaattt actgagaagc ctgtaactga ataccccccg gtgatacagg ataatacagt
     7321 gaccactaaa gactatctgt acagcaataa tattgatact gaccttttag tggttatcgg
     7381 agggcttatt gttatattgt ttctcatgat gcttatcatg tactttatta attctctcag
     7441 gcataagaaa ttaactgaaa tactgggaaa aatatctttt cctgaaaaat ccgggagcaa
     7501 tttgcttaaa ccggaggcgg tcagctgtaa tttgttgtcc ctgtgtgtgc tggcaccggt
     7561 actctgtaca cccagaaagc tggcagaagt ctccagggag atcatcgtgg cgagtgcgca
     7621 ggaaattgca tggacgccgg ggagcagtcg tattgatggt catcagggcg aaaatattac
     7681 ctggtctctg tcatcgcagt ctcctgccga taagtatgat ctcatcaata aaacgtcccg
     7741 tgtttctcct gacagtggcc gtttttatga accggacaca gttctggatc accgtgaacg
     7801 ggtacgcctt tttattcaga tccagtttcc gcacgggagt gactggtctg aagtattgcg
     7861 cctgactgac attgttacag agaaggtctc ttccggcagg gctgcaggcg ggtatcacca
     7921 ccggcataca gggtacacct ggtctgtttc ccacatcatc cttttctgcc ctgttccaga
     7981 ttttcccgga gtttcatcat gagttttaac gcaaaggata tgacccaggg cggtcagatt
     8041 gcgtccatgc gtatccgcat gttcagccag atcgccaata tcatgcttta ctgcctgttt
     8101 atttttttct ggatactcgt tggtctgatt ttatgggtaa aaataagctg gcagacgttt
     8161 gtgaacggct gtatttactg gtggtgtacc acgctggaag gcatgcggga tttaatcaag
     8221 tcccagccag tatatgagat tcagtattac ggcaaaacct tccggatgaa cgctgcccag
     8281 gtactgcatg ataaatatat gatctggtgc ggtgagcagc tatggtccgc attcgttctg
     8341 gcgtctgtcg tggccctggt tatttgcctg atcaccttct ttgttgtctc ctggattctg
     8401 gggcgtcagg gtaaacaaca gagcgaaaat gaagtcacag gtggtcgtca gctgacagac
     8461 aatccgaaag acgttgcccg gatgctgaaa aaagacggca aggactccga tatccggatt
     8521 ggcgacctgc cgattatccg ggattccgaa atccagaact tctgtctgca cggcacggtc
     8581 ggggccggta agtcggaggt tatccgtcgt ctggccaact atgcccgtca gcgcggtgat
     8641 atggtggtga tttatgaccg ttcaggggaa tttgttaaaa gttactatga tccctccatc
     8701 gataaaatcc tgaatccgct ggatgcccgc tgtgccgcat gggatttgtg gaaggagtgc
     8761 ctgacacagc cggattttga taataccgca aatactctga tccctatggg tacaaaagag
     8821 gacccgttct ggcagggttc aggacgtacc atttttgcgg aagcggcgta cctgatgcgt
     8881 aatgacccca accgcagcta cagcaaactg gtggacacac tgctttccat caaaattgaa
     8941 aaactgcgta ccttcctgcg taattcaccg gcggccaacc tggtggaaga gaaaattgag
     9001 aaaacggcga tttccatccg tgctgtgctg accaactacg tgaaagccat ccgttacctg
     9061 caggggattg agcataacgg tgagcccttc accatccgtg actggatgcg tggtgtccgg
     9121 gaagatcaga aaaacggctg gctgtttatt tcgtcgaacg ccgacaccca tgcctccctg
     9181 aagcctgtga tctccatgtg gctgtccatt gccattcgtg gtctgctggc aatgggagaa
     9241 aaccgtaacc gtcgtgtgtg gtttttctgt gatgagttac ccacgttaca caaactgccg
     9301 gacctggtgg agatcctgcc ggaagcccgt aagttcggtg gctgttatgt gtttggtatc
     9361 cagtcctatg cccagctgga agatatctac ggtgagaaag cggcggccac gctgtttgac
     9421 gtcatgaaca cccgtgcttt tttccgttcc ccgagccata agattgcaga gttcgctgcc
     9481 ggtgagattg gtgagaaaga gcacctgaaa gccagcgagc agtattccta cggtgctgac
     9541 ccggtacgtg acggggtatc gaccggtaag gatatggagc gtcagacact ggtcagttat
     9601 tccgacattc agtctctgcc ggatctgacc tgctatgtca ctctgcccgg accgtatccg
     9661 gcagtaaaac tctccctgaa atatcaggca cgaccgaagg tcgctccgga gtttattccg
     9721 cgtgacatca acccggaaat ggagaaccgt ctgagtgccg tacttgctgc aagggaagca
     9781 gaaggtcgcc agatggccag cctcttcgaa ccggaggttg cctccggaga aggcgtgact
     9841 caggctgaac agccacaaca gccacaacag ccacaacagc cacaacagcc acaacagcca
     9901 caacagccac aacagccaca acagccacaa cagccagtgt cttctgtcat caatgataag
     9961 aagtcagatg caggtgtgag tgttccggca ggggggatcg agcaggagct gaaaatgaaa
    10021 ccggaagaag agatggaaca gcaactgcca cccgggatca gtgaatccgg tgaagtggtg
    10081 gatatggccg cttatgaggc atggcaacag gaaaatcatc cggacatcca gcagcagatg
    10141 cagcgtcgtg aagaggtgaa cattaatgtg caccgggagc gcggggagga tgttgagccg
    10201 ggagatgatt tctgatgttg agtttcagcg ttgttaaatc ggcaggcagt gccggaaact
    10261 attacaccga caaggataat tactatgtgc tgggcagcat gggagaacgc tgggccggtc
    10321 agggggctga acagcttggg ctgcagggca gcgtcgataa ggatgttttt acccgtcttc
    10381 tggagggcag gctgccggac ggggcggatt taagccgcat gcaggatggc agtaataagc
    10441 atcgtcccgg ctacgacctg accttctccg cccccaaaag tgtctccatg atggccatgc
    10501 tcggggggga taaacgtctt attgatgcac ataaccaggc tgtggatttt gctgtccgtc
    10561 aggtggaggc gctggcttcc acacgggtga tgacggacgg acagtcagaa acggtgctga
    10621 ccggtaatct ggtgatggca ctgtttaacc atgacaccag tcgcgatcag gacccacagt
    10681 tacacacgca tgtggtggtg gctaatgtca cgcagcataa cggcgaatgg aagacactga
    10741 gcagtgacaa agtggggaaa accggatttt ccgagaacgt gctcgcgaac cggattgcgt
    10801 tcgggaaaat atatcagagt gagttacgtc agcgcgttga ggcgctgggt tatgaaaccg
    10861 aagtggtcgg taagcacggt atgtgggaga tgccgggcgt accggtggaa gccttctccg
    10921 gacgctcaca ggctatccgg gaggccgtcg gtgaagacgc ctcgctgaaa tcccgggatg
    10981 tggcggcact ggatacgcgt aaatccaaac agcacgtcga tcctgaagtc agaatggctg
    11041 aatggatgca gacgctgaag gaaaccgggt tcgacatccg ggcatatcgt gacgcggcgg
    11101 atcagcgtgc ggagatccgc acgcaggcgc ccgggcctgc ttcacaggac gggccggatg
    11161 tgcagcaggc ggtgacacag gccattgccg gattaagtga acgaaaagtg cagttcacgt
    11221 atacggacgt gctggccagg acggtcggca tactgccgcc ggaaaatggt gtgattgaac
    11281 gggcacgcgc cggtatcgat gaggccatca gccgtgagca gcttatcccc ctcgatcgtg
    11341 agaaggggct gttcacgtcc gggattcatg tgctcgatga gctgtcagtc cgggcactca
    11401 gtcgtgacat catgaaacag aaccgggtga ccgtacatcc ggagaaaagt gtcccccgga
    11461 cggccggtta cagcgatgcc gtcagtgttc tggcacagga tcgcccgtcg ctggccattg
    11521 tgtccgggca gggcggtgca gccgggcagc gtgagcgggt ggctgaactg gtcatgatgg
    11581 cccgggagca ggggcgggag gtgcagatta tcgctgctga ccgtcgctcg cagatgaacc
    11641 tgaagcagga tgaacggctg tccggtgaac tgataaccgg acgtcgtcag ttgctggaag
    11701 gcatggcctt cccgccgggc agtaccgtta tcgtcgacca gggcgaaaaa ctctccctga
    11761 aagagacgtt aaccctgctg gatggtgccg cacgtcataa cgtacaggtc ctgataaccg
    11821 acagcgggca gcgaaccggt acaggcagtg cactgatggc catgaaggat gccggggtga
    11881 acacatatcg ctggcagggg ggagaacagc gaccggccac catcatcagt gaaccggacc
    11941 gtaatgtccg ctatgcccgg ctggccggag attttgcggc cagcgtgaaa gccggagaag
    12001 agagcgtggc acaggtcagc ggggtacggg aacaggccat actgacagag gccattcgca
    12061 gtgagctgaa aacgcagggc gtgctcggac accctgaggt gaccatgact gcactgtcac
    12121 cggtctggct ggacagccgg agccgttatc tgcgggatat gtaccgaccg gggatggtga
    12181 tggagcagtg gaacccggag acacgcagtc atgaccgcta tgtgatagac cgggtgacag
    12241 cgcagagtca cagcctgacc ctgcgggatg cgcagggcga aacgcaggtg gtgcgtattt
    12301 cctccctgga cagcagctgg tcgctgttcc ggccggaaaa aatgccggtg gcagacggcg
    12361 agcgactgag ggtgacaggg aaaattcccg gactccgcgt ctccggcggt gaccgcctgc
    12421 aggtggcatc cgtcagtgaa gatgcgatga cggttgttgt gccggggcgg gctgaaccgg
    12481 ccaccctgcc tgtggctgat tcaccattca cggcactgaa gctggagaac ggctgggtgg
    12541 aaacgcccgg gcattccgtc agcgacagtg cgacggtttt tgcctccgtc acacagatgg
    12601 cgatggacaa cgcgaccctg aacggtctgg cccgcagtgg tcgtgatgtc cggctgtatt
    12661 cctcgctgga tgaaccccgt actgcggaaa aacttgcccg ccatccctcc tttacggtgg
    12721 tttctgatca gataaaggcg cgtgccggtg agacattgct ggaaaccgct atcagtctgc
    12781 agaaagccgg gcttcacacg ccggcacagc aggccattca tctggccctt cctgtgctgg
    12841 aaagtaaaaa cctggccttc agcatggtgg acctgctgac agaggcgaag tcgtttgctg
    12901 cagaaggaac cggttttact gaactgggag gggaaatcaa tgcgcagata aaacgcggtg
    12961 acttactgta tgtggatgtg gcaaaaggct atggcacagg tctgctggtt tcccgtgcgt
    13021 cgtatgaggc agaaaaaagt attcttcgcc atattctcga aggtaaggag gcggtcacgc
    13081 cgttgatgga gagagtaccc ggcgaactca tggagacgtt aacgtcggga cagcgtgccg
    13141 ccacccgtat gatactggaa acgtccgacc gtttcacggt ggtacagggt tatgccggtg
    13201 tgggtaagac cacacagttc cgggcggtga tgtcagccgt gaacatgctg ccggcgagtg
    13261 agcgtccccg agttgtgggg ctggggccca cgcaccgtgc ggtcggtgag atgcgcagcg
    13321 ccggcgtgga tgcacagacg ctggcgtcct ttctgcatga cacgcagctg cagcagcgca
    13381 gcggagaaac gccggatttc agcaacacgc tgttcctgct cgatgagagc tcaatggtgg
    13441 gcaataccga tatggcacgg gcatacgccc tgattgcggc cggtggcggt cgtgccgtgg
    13501 ccagtggtga cacggaccag ctgcaggcca tcgcgcccgg tcagcctttc cgtctgcagc
    13561 agacgcgcag tgctgccgat gtggtcatca tgaaggagat tgtgcgtcag acgccggaac
    13621 tgcgggaggc ggtatacagc ctgattaacc gggatgtgga aagggcactg tccgggcttg
    13681 agagtgtgaa accgtctcag gttccccgtc aggagggcgc atgggcaccg gagcactccg
    13741 tgacggagtt cagtcacagc caggaagcga aactggcaga agcgcagcag aaggcgatgc
    13801 tgaaaggcga ggctttcccg gatatcccca tgacactgta tgaggccatt gtccgcgact
    13861 ataccggcag gacaccggaa gcacgggagc agacgctgat tgtcacgcac ctgaatgagg
    13921 accggcgcgt actgaacagc atgattcatg atgcacggga aaaggccggt gagctgggca
    13981 aagagcaggt catggtgcct gtcctgaaca cagcgaatat acgtgacgga gaactgcgtc
    14041 gtctctccac ctgggagaat aacccggatg cccttgccct ggtggatagt gtgtatcacc
    14101 ggattgccgg tatcagcaag gatgacgggc tgataaccct ggaggatgcg gaaggtaaca
    14161 cgcggctgat ttcgccccgg gaggcggtgg ctgaaggtgt cacactgtac acaccagaca
    14221 aaatccgggt gggaaccggt gaccggatgc gcttcacgaa gagtgaccgg gagcgcggtt
    14281 atgtggccaa cagcgtctgg acggtgacag cggtttccgg tgacagtgtc acgctgtcgg
    14341 acggacagca gacccgggtg attcgccccg gtcaggagcg ggcagagcaa catattgacc
    14401 tggcctatgc catcaccgcc cacggtgcgc aggggacaag tgaaaccttt gccattgcgc
    14461 ttgaaggcac ggaaggtaac cggaaactga tggcaggctt tgagtcagcc tacgtggccc
    14521 tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa ccgtcagggc tggacggatg
    14581 ccattaacaa tgccgtacag aaaggaacag cccacgatgt gtttgagccg aaaccggacc
    14641 gggaggtcat gaatgcgcag cggctgttca gtacggcacg ggaactgcgg gacgtggcgg
    14701 caggccgtgc cgttctccgt caggcggggc tggccggggg agacagccct gcacggttta
    14761 ttgcaccggg acgtaaatat ccgcagccgt atgtggcact gccggcgttt gaccgtaacg
    14821 gcaggtccgc cggtatctgg ctgaacccac tgaccacgga tgacggaaac gggctgcggg
    14881 gattcagcgg tgaaggacgg gtgaaaggcc gcggggatgc gcagttcgtg gccctgcagg
    14941 gcagccgtaa cggagagagc ctgctggctg ataatatgca ggatggtgtc cggattgccc
    15001 gtgataatcc cgacagcggt gtggtggtaa gaattgccgg tgaaggtcgt ccgtggaatc
    15061 ccggtgccat caccggtggt cgcgtgtggg gggatatccc ggacagcagc gtccagccgg
    15121 gggccggaaa tggcgaaccg gtcacggcag aggtgctggc acagcggcag gctgaagagg
    15181 ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg gcagagaaca
    15241 aacctgacct gccggacggc aaaacagagc tggctgtcag ggatattgcc gggcaggaac
    15301 gtgaccggac tgccagttct gaacgggaaa ccgcactgcc ggagagtgtg ctgcgtgaat
    15361 cacaacggga acgggaagcg gtccgggagg ttgcccggga aaatctgctg caggagcgtc
    15421 tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc ctgggcggag
    15481 actgatacag gaagacaaac actgatgaca acggataaca cgaacacgac acgtaacgat
    15541 tcactggctg cacggaccga tacctggttg cagtcatttc tggtctggtc acccggacag
    15601 cgggatatca tcaaaacggt ggcactggtg ctgatggtac tggaccacat caatctgata
    15661 ttccagctga agcaggaatg gatgtttctg gccgggcgag gagcctttcc gctgtttgcc
    15721 ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc cattaaccgg
    15781 ctgtggggat ggggaattat tgcccagttc gcatattatc tggccggctt tccctggtat
    15841 gaggggaata tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg gtgtgaaacg
    15901 cgcagtgggt ggcgtacagc agcggccatt cttctgatgg ccctgtgggg gcctttgtcc
    15961 ggcaccagtt acggcattgc cgggctgctg atgctggcag tcagccaccg gctgtaccgg
    16021 gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc tgctggctgt tattccggcg
    16081 cttaaccttg ccaccagtga tgcagcggcg gtggccggtc ttgtgatgac ggtgctgacc
    16141 gttggtctgg tgtcgtgtgc aggaaaatca ttaccccgtt tctggcccgg ggattttttc
    16201 ccggtattct acgcctgtca tctggctgtg ctgggcgttc tggcgctgtg acgggtgtgg
    16261 tatctttggc cgcaagagga tgattcgtca gaggcagaac acagcatgac agagcagaag
    16321 cgaccggtac tgacactgaa gcggaaaacg gaaggggaga cgcctgtccg cagccggaaa
    16381 accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa gctggccgag
    16441 aaagcagccc gggaagcaga gctggcggca aaaaaagcgc aggccagaca ggcgctgtcc
    16501 atttatctga acctgcccac actggatgag gctgtgaaca ccctgaagcc ctggtggccg
    16561 ggattatttg acggtgacac gccccggctt ctggcctgcg gtatccggga cgtgttactg
    16621 gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa aactgcgcag ggcgctgaag
    16681 gccatcaccc gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg ccggtatgat
    16741 acggaagggt atgtgacgga gcatatttct caggaggagg aagcgtatgc ggcagcgcgt
    16801 ctggataaaa tccgccgcca gaaccggata aaggcagaac ttcaggccgt gcttgatgag
    16861 aaataaaaaa agcctccctc agggagggga ggcagggaaa taaggcttaa aaggaaataa
    16921 cttccgtacc cgaaagaaag ccattccact gagctattta tcacaataat actgacaact
    16981 aaagggggac agaaatatga atggattcag aaacagttca cggaacggtc aggtctggtg
    17041 ctggcagcgt gccggaagcc gggccgttat tctggaggtc agtggacgca ggatggaagc
    17101 ggcagaagca tggcgacggg ctgccggtgt tgcgccccgg acagactggc agcagtttgc
    17161 ccgaaaaagg gctgcacact gccaccggcg ttgccgggga agggggtaac gaaaaaagca
    17221 gccttcatta ctccaggagt cagaaggctg aaagaagttt atgagatttg ctttttttaa
    17281 tatccggtcg ggaagtatga ccggacagtt ctgagtagca cagatttttt tccgtattaa
    17341 gtcgagattt tatattttgt gattaatttc acaaaataag gtgttgttca atgtgtgctg
    17401 taatattcag gatgtctgaa atacaggtgc catttatttg atatggagag taacatgagg
    17461 aaatatattc cacgggtatt atttatcttt tcatggcggt attaacttcc ctagtttcat
    17521 cttcatacct tattgggtgt attaaaaaca gtaggctgcc aaagtcttaa acaagtttca
    17581 attctccatg acagttcaac atcagccaga ggaaattccc attctggacc agcgggagca
    17641 tacgaacaat aattttcggc ttcgcgctat agcgggctca
//
LOCUS       DAOFSV010000088        17519 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.088, whole genome shotgun sequence.
ACCESSION   DAOFSV010000088 DAOFSV010000000
VERSION     DAOFSV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.088"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            53..1522
                     /gene="gspA"
                     /locus_tag="QBH27_003102"
     CDS             53..1522
                     /gene="gspA"
                     /locus_tag="QBH27_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDQ7015383.1"
                     /translation="MSTRREVILSWLREKHQTWRLCYLLGEAGSGKTRLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTCVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLPSFTAPLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1524..1943
                     /gene="gspB"
                     /locus_tag="QBH27_003103"
     CDS             1524..1943
                     /gene="gspB"
                     /locus_tag="QBH27_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDQ7015384.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEDNTVEESDEKAGLRERVKNALNELER"
     gene            2181..2492
                     /gene="rpsJ"
                     /locus_tag="QBH27_003104"
     CDS             2181..2492
                     /gene="rpsJ"
                     /locus_tag="QBH27_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDQ7015385.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2525..3154
                     /gene="rplC"
                     /locus_tag="QBH27_003105"
     CDS             2525..3154
                     /gene="rplC"
                     /locus_tag="QBH27_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDQ7015386.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3165..3770
                     /gene="rplD"
                     /locus_tag="QBH27_003106"
     CDS             3165..3770
                     /gene="rplD"
                     /locus_tag="QBH27_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDQ7015387.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3767..4069
                     /gene="rplW"
                     /locus_tag="QBH27_003107"
     CDS             3767..4069
                     /gene="rplW"
                     /locus_tag="QBH27_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDQ7015388.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4087..4908
                     /gene="rplB"
                     /locus_tag="QBH27_003108"
     CDS             4087..4908
                     /gene="rplB"
                     /locus_tag="QBH27_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDQ7015389.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4925..5203
                     /gene="rpsS"
                     /locus_tag="QBH27_003109"
     CDS             4925..5203
                     /gene="rpsS"
                     /locus_tag="QBH27_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDQ7015390.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5218..5550
                     /gene="rplV"
                     /locus_tag="QBH27_003110"
     CDS             5218..5550
                     /gene="rplV"
                     /locus_tag="QBH27_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDQ7015391.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5568..6269
                     /gene="rpsC"
                     /locus_tag="QBH27_003111"
     CDS             5568..6269
                     /gene="rpsC"
                     /locus_tag="QBH27_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDQ7015392.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6282..6692
                     /gene="rplP"
                     /locus_tag="QBH27_003112"
     CDS             6282..6692
                     /gene="rplP"
                     /locus_tag="QBH27_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDQ7015393.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6692..6883
                     /gene="rpmC"
                     /locus_tag="QBH27_003113"
     CDS             6692..6883
                     /gene="rpmC"
                     /locus_tag="QBH27_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDQ7015394.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6883..7137
                     /gene="rpsQ"
                     /locus_tag="QBH27_003114"
     CDS             6883..7137
                     /gene="rpsQ"
                     /locus_tag="QBH27_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDQ7015395.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7302..7673
                     /gene="rplN"
                     /locus_tag="QBH27_003115"
     CDS             7302..7673
                     /gene="rplN"
                     /locus_tag="QBH27_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDQ7015396.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7684..7998
                     /gene="rplX"
                     /locus_tag="QBH27_003116"
     CDS             7684..7998
                     /gene="rplX"
                     /locus_tag="QBH27_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDQ7015397.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8013..8552
                     /gene="rplE"
                     /locus_tag="QBH27_003117"
     CDS             8013..8552
                     /gene="rplE"
                     /locus_tag="QBH27_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDQ7015398.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8567..8872
                     /gene="rpsN"
                     /locus_tag="QBH27_003118"
     CDS             8567..8872
                     /gene="rpsN"
                     /locus_tag="QBH27_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDQ7015399.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8906..9298
                     /gene="rpsH"
                     /locus_tag="QBH27_003119"
     CDS             8906..9298
                     /gene="rpsH"
                     /locus_tag="QBH27_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDQ7015400.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9311..9844
                     /gene="rplF"
                     /locus_tag="QBH27_003120"
     CDS             9311..9844
                     /gene="rplF"
                     /locus_tag="QBH27_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDQ7015401.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9854..10207
                     /gene="rplR"
                     /locus_tag="QBH27_003121"
     CDS             9854..10207
                     /gene="rplR"
                     /locus_tag="QBH27_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDQ7015402.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10222..10725
                     /gene="rpsE"
                     /locus_tag="QBH27_003122"
     CDS             10222..10725
                     /gene="rpsE"
                     /locus_tag="QBH27_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDQ7015403.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10729..10908
                     /gene="rpmD"
                     /locus_tag="QBH27_003123"
     CDS             10729..10908
                     /gene="rpmD"
                     /locus_tag="QBH27_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDQ7015404.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10912..11346
                     /gene="rplO"
                     /locus_tag="QBH27_003124"
     CDS             10912..11346
                     /gene="rplO"
                     /locus_tag="QBH27_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDQ7015405.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11354..12685
                     /gene="secY"
                     /locus_tag="QBH27_003125"
     CDS             11354..12685
                     /gene="secY"
                     /locus_tag="QBH27_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDQ7015406.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12717..12833
                     /gene="rpmJ"
                     /locus_tag="QBH27_003126"
     CDS             12717..12833
                     /gene="rpmJ"
                     /locus_tag="QBH27_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDQ7015407.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12980..13336
                     /gene="rpsM"
                     /locus_tag="QBH27_003127"
     CDS             12980..13336
                     /gene="rpsM"
                     /locus_tag="QBH27_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDQ7015408.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13353..13742
                     /gene="rpsK"
                     /locus_tag="QBH27_003128"
     CDS             13353..13742
                     /gene="rpsK"
                     /locus_tag="QBH27_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDQ7015409.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADVVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13776..14396
                     /gene="rpsD"
                     /locus_tag="QBH27_003129"
     CDS             13776..14396
                     /gene="rpsD"
                     /locus_tag="QBH27_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDQ7015410.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14422..15411
                     /gene="rpoA"
                     /locus_tag="QBH27_003130"
     CDS             14422..15411
                     /gene="rpoA"
                     /locus_tag="QBH27_003130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDQ7015411.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15452..15835
                     /gene="rplQ"
                     /locus_tag="QBH27_003131"
     CDS             15452..15835
                     /gene="rplQ"
                     /locus_tag="QBH27_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDQ7015412.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15942..16310
                     /locus_tag="QBH27_003132"
     CDS             15942..16310
                     /locus_tag="QBH27_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDQ7015413.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16321..16746
                     /gene="zntR"
                     /locus_tag="QBH27_003133"
     CDS             16321..16746
                     /gene="zntR"
                     /locus_tag="QBH27_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDQ7015414.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16802..17020
                     /gene="arfA"
                     /locus_tag="QBH27_003134"
     CDS             16802..17020
                     /gene="arfA"
                     /locus_tag="QBH27_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDQ7015415.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(17017..17430)
                     /gene="mscL"
                     /locus_tag="QBH27_003135"
     CDS             complement(17017..17430)
                     /gene="mscL"
                     /locus_tag="QBH27_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDQ7015416.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
BASE COUNT         4511 a         4060 c         4683 g         4265 t
ORIGIN      
        1 catacattat ttttatgatt aaggataatc aataattaaa acaggaagtt ctatgtctac
       61 gagaagagaa gtcattcttt cctggttgcg tgagaagcat cagacctggc gtctgtgcta
      121 tttgttgggt gaggctggaa gtggaaaaac ccggctggcg cagcaactac aaaaagataa
      181 acatcgccgt gtgattactt taagcctcgt tgtttcctgg caaggtaagg ccgcatggat
      241 cgttaccgac gataatgcgg ctgaacaggg ctgccgtgac agcgcctgga cgcgagatga
      301 gatggcgggt caattactgc atgcacttca tcgaactgat agtcgctgcc ctcttattat
      361 tattgaaaat gctcacctga atcaccgccg catacttgat gatctccaac gcgcgatatc
      421 tcttattcct gacggtcaat ttttgctgat cggcagaccc gatcgaaaag tcgaacgtga
      481 ttttaaaaaa cagggcattg aacttgtctc gacaggtcgt ctgacggagc acgagcttaa
      541 ggcaagcatc cttgaaggtc agaatattga ccagcccgat ctccttttaa ccgccagagt
      601 tctgaaacgg atagctttat tatgtcgggg agatcgcaga aagctggcgc ttgccggcga
      661 aacaattagc ttattacagc aagccgagca gacctgcgtt ttcactgcaa aacaatggcg
      721 aatgatttac cgcgtattag gtgataaccg cccccggaaa atgcagcttg ccgtggtgat
      781 gagtggaacc attatcgcac ttacctgtgg ttggctactg ctgccctctt tcacagctcc
      841 gttacccgtt cctgcgtggc tgatacccgt aacgccagta gttaaacaag atatgactaa
      901 ggatattgcc catgtggtga tgcgcgatag tgaggcgcta agcgtgttat acggcgtatg
      961 ggggtacgag gtcccagccg attcggcctg gtgcgatcag gcagtaagag cggggctggt
     1021 atgtaaaagt gggaatgctt ctttacaaac attggtagat cagaatttgc cctggatcgc
     1081 atcgcttaaa gtcggggaca aaaaattgcc tgttgtggtc gttcgcgtcg gtgatgctac
     1141 cgttgatgta ctcgtagggc agcaaacctg gacgctgaca cataaatggt ttgagttggt
     1201 atggacgggt gattatcttc tgttgtggaa aatgtcgccg gaaggagaga gtaccattac
     1261 gcgtgatagc agcgaggaag agattctctg gctggaaacg atgttaaacc gcgcattgca
     1321 tatttcgaca gaatcttcgg cagagtggcg tcccttgttg gtagaaaaaa taaagcaatt
     1381 tcaaaaaagc catcacttga aaactgacgg tgttgttggc ttttcaacgt tagtgcatct
     1441 ctggcaggtg gcaggggaga gtgcctactt atatcgggat gaagcaaata tttccccgga
     1501 aacaacagtg aaggggaaat gacatgtttg aattctatat cgccgcgcgt gagcagaaag
     1561 aaacaggtca ccctggtatc ttctcccgac aaaaacacag caccataata tatgtcattt
     1621 gtttattgct gatttgcctg tggtttgcag gaatggtgtt ggttggtggg tatgcccggc
     1681 agctatgggc actttggata gtaaaagcag aagtcactgt agatgctgaa acgcccgctt
     1741 ttaaacaatc aacacaacac tattttttca aaaaacagcc actccccgtc gtcgaatccg
     1801 tcgaggagga agatgaccca ggagtcgcgg ttgagaatgc cccctcttct tctgaagacg
     1861 aagacaatac tgtagaagaa agcgacgaga aagccggttt aagagagcgt gtcaaaaatg
     1921 cgctgaacga acttgagaga taacccaaag gctgtttact ttactagcaa tacgcttgcg
     1981 ttcggtggtt aagtatgtat aatgcgcggg cttgtcgtag ttgacagcag gttcaatctg
     2041 aacctcagtt agccgaattt ggctacctaa caatgctccc aatcggggag ctacgtaaga
     2101 acggttacac tctcccatca atcgtaatgg gtctgaggag taatcatttt cgtttataaa
     2161 ataattggag ctctggtctc atgcagaacc aaagaatccg tatccgcctg aaagcgtttg
     2221 atcatcgtct gatcgatcaa gcaaccgcgg aaatcgtcga gactgctaag cgcactggtg
     2281 cgcaggtccg tggtccgatc ccgctgccga cacgcaaaga gcgcttcact gttctgatct
     2341 ccccgcacgt caacaaagac gcgcgcgatc agtacgaaat ccgtactcac ttgcgtctgg
     2401 ttgacatcgt tgagccaacc gagaaaaccg ttgatgctct gatgcgtctg gatctggctg
     2461 ccggtgtaga cgtgcagatc agcctgggtt aatcaggtca ttgagcgatt gagaggttga
     2521 aacaatgatt ggtttagtcg gtaaaaaagt gggtatgacc cgtatcttca cagaagacgg
     2581 cgtttctatc ccagtaaccg taatcgaagt tgaagcaaac cgcgttactc aggttaaaga
     2641 cctggctaac gatggctacc gtgctattca ggtgaccacc ggtgctaaaa aagctaaccg
     2701 tgtgaccaag cctgaagctg gccacttcgc taaagctggc gtagaagctg gccgtggtct
     2761 gtgggaattc cgcctggctg aaggcgaaga gttcactgta ggtcagagca ttagcgttga
     2821 actgtttgct gacgttaaaa aagttgacgt aactggcacc tctaaaggta aaggtttcgc
     2881 aggtaccgtt aagcgctgga acttccgtac ccaggacgct actcacggta actccttgtc
     2941 tcaccgcgtt ccgggttcta tcggtcagaa ccagactccg ggcaaagtgt tcaaaggcaa
     3001 gaaaatggca ggtcagatgg gtaacgaacg tgtaaccgtt cagagccttg acgtagtacg
     3061 cgttgacgct gagcgcaacc tgctgctggt taaaggcgct gtcccgggtg caaccggtag
     3121 cgacctgatc gttaaaccag ctgtgaaggc gtaaggagat agcaatggaa ttagtattga
     3181 aagacgcgca gagcgcgctg actgtttccg aaactacctt cggtcgtgat ttcaacgaag
     3241 cgctggttca ccaggttgtt gttgcttatg cagctggtgc tcgtcagggt actcgtgctc
     3301 agaagactcg tgctgaagta actggttccg gtaaaaaacc gtggcgccag aaaggcaccg
     3361 gccgtgcgcg ttctggttct atcaagagcc cgatctggcg ttctggtggc gtgacctttg
     3421 ctgctcgtcc gcaggaccac agtcaaaaag ttaacaagaa gatgtaccgc ggcgcgctga
     3481 aaagcatcct gtccgaactg gtacgtcagg atcgtctgat cgttgtcgag aagttctctg
     3541 tagaagcgcc gaaaactaag ctgctggcac agaaactgaa agacatggct ctggaagatg
     3601 tgctgatcat caccggtgag ctggacgaga acctgttcct ggctgcgcgc aacctgcaca
     3661 aggttgacgt acgcgatgca actggtatcg acccggttag cctgatcgcc ttcgacaaag
     3721 tcgtaatgac tgctgatgct gttaagcaag ttgaggagat gctggcatga ttcgtgaaga
     3781 acgtctgctg aaggtgctgc gtgcaccgca cgtttctgaa aaagcgtcta ctgcgatgga
     3841 aaaatccaac accatcgtac tcaaagttgc taaagacgcg accaaagcag aaatcaaagc
     3901 tgctgtgcag aaactgtttg aagtcgaagt cgaagtcgtt aacaccctgg tagttaaagg
     3961 gaaagttaaa cgtcacggac agcgtatcgg tcgtcgtagc gactggaaaa aagcttacgt
     4021 caccctgaaa gaaggccaga atctggactt cgttggcggc gctgagtaag tcggaggagt
     4081 aatacaatgg cagttgttaa atgtaaaccg acatctccgg gtcgtcgcca cgtagttaaa
     4141 gtggttaacc ctgagctgca caagggcaaa ccttttgctc cgttgctgga aaaaaacagc
     4201 aaatccggtg gtcgtaacaa caatggccgt atcaccactc gtcatatcgg tggtggccac
     4261 aagcaggctt accgtattgt tgacttcaaa cgcaacaaag acggtatccc ggcagttgtt
     4321 gaacgtcttg agtacgatcc gaaccgttcc gcgaacatcg cgctggttct gtacaaagac
     4381 ggtgaacgcc gttacatcct ggcccctaaa ggcctgaaag ctggcgacca gattcagtct
     4441 ggcgttgatg ctgcaatcaa accaggtaac accctgccga tgcgcaacat cccggttggt
     4501 tctactgttc ataacgtaga aatgaaacca ggtaaaggcg gtcagctggc acgttccgct
     4561 ggtacttacg ttcagatcgt tgctcgtgat ggtgcttatg tcaccctgcg tctgcgttct
     4621 ggtgaaatgc gtaaagtaga agcagactgc cgtgcaactc tgggcgaagt tggcaatgct
     4681 gagcatatgc tgcgcgttct gggtaaagca ggtgctgcac gctggcgtgg tgttcgtccg
     4741 accgttcgcg gtaccgcgat gaacccggta gaccacccac atggtggtgg tgaaggtcgt
     4801 aactttggta agcacccggt aactccgtgg ggcgttcaga ccaaaggtaa gaagacccgc
     4861 agcaacaagc gtactgataa attcatcgta cgtcgccgta gcaaataatt ttagaggata
     4921 agccatgcca cgttctctca agaaaggtcc ttttattgac ctgcacttgc tgaagaaggt
     4981 agagaaagcg gtggaaagcg gagacaagaa gcccctgcgc acttggtccc gtcgttcaac
     5041 gatctttcct aacatgatcg gtttgaccat cgctgtccat aatggtcgtc agcacgttcc
     5101 ggtatttgta accgacgaaa tggtcggtca caaactgggt gaattcgcac cgactcgtac
     5161 ttatcgcggc cacgctgctg ataaaaaagc gaagaagaaa taaggtagga ggaagagatg
     5221 gaaactatcg ctaaacatcg ccatgctcgt tcttctgctc agaaggttcg ccttgttgct
     5281 gacctgattc gcggtaagaa agtgtcgcag gctctggata ttttgaccta caccaacaag
     5341 aaagcggctg tactggtcaa gaaagttctg gaatctgcca ttgctaacgc tgaacacaac
     5401 gatggcgctg acattgacga tctgaaagtt acgaaaattt tcgtagacga aggcccgagc
     5461 atgaagcgca ttatgccgcg tgcaaaaggt cgtgcagatc gcatcctgaa gcgcaccagc
     5521 cacatcactg tggttgtgtc cgatcgctga gactctggag actagcaatg ggtcagaaag
     5581 tacatcctaa tggtattcgc ctgggtattg taaaaccatg gaactctacc tggtttgcga
     5641 acaccaaaga attcgctgac aacctggaca gcgattttaa agtacgtcag tacctgacta
     5701 aggaactggc taaagcgtcc gtatctcgta tcgttatcga gcgtccggct aagagcatcc
     5761 gtgtaaccat tcacactgct cgcccgggta tcgttatcgg taaaaaaggt gaagacgtag
     5821 aaaaactgcg taaggtcgta gcggacatcg ctggcgttcc tgcacagatc aacatcgccg
     5881 aagttcgtaa gcctgaactg gacgcaaaac tggttgctga cagcatcact tctcagctgg
     5941 aacgtcgcgt tatgttccgt cgtgctatga agcgtgctgt acagaacgca atgcgtctgg
     6001 gcgctaaagg tattaaagtt gaagttagcg gccgtctggg cggcgcggaa atcgcacgta
     6061 ccgaatggta ccgcgaaggt cgcgtaccgc tgcacactct gcgtgctgac atcgactaca
     6121 acacctctga agcgcacacc acttacggtg taatcggcgt taaagtgtgg atcttcaaag
     6181 gcgagatcct gggtggtatg gctgctgttg aacaaccgga aaaaccggct gctcagccta
     6241 aaaagcagca gcgtaaaggc cgtaaataag gagcgtcgct gatgttacaa ccaaagcgta
     6301 caaaattccg taaaatgcac aaaggccgta accgcggtct ggcgcagggt acggatgtta
     6361 gcttcggcag cttcggtctg aaagctgttg gccgtggtcg tctgactgca cgtcagatcg
     6421 aagcagcacg tcgtgctatg acccgtgcag ttaagcgtca aggtaagatc tggatccgtg
     6481 tgttcccgga caaaccgatc actgaaaagc cgctggcagt gcgtatgggt aaaggtaaag
     6541 gtaacgtgga gtattgggtt gccttgattc agccgggtaa agtcctgtat gaaatggacg
     6601 gtgttccgga agagctggcc cgtgaagcat tcaagctggc agcagcgaaa ctgccgatta
     6661 aaaccacctt tgtaactaag acggtgatgt aatgaaagca aaagagctgc gtgagaagag
     6721 cgttgaagag ctgaacaccg agctgctgaa cctgctgcgt gagcagttca acctgcgtat
     6781 gcaggctgca agtggccagc tgcaacagtc tcacctgttg aagcaagtgc gtcgcgatgt
     6841 cgcacgcgtt aagactttac tgaacgagaa ggcgggtgcg taatgaccga taaaatccgt
     6901 actctgcaag gtcgcgttgt tagcgacaaa atggagaaat ccattgttgt tgctatcgaa
     6961 cgttttgtga aacacccgat ctacggtaaa ttcatcaagc gtacgaccaa actgcacgta
     7021 catgacgaga acaacgaatg cggtatcggt gacgtggttg aaatccgcga atgccgtccg
     7081 ctgtccaaga ctaaatcctg gacgctggtt cgcgttgtag agaaagcggt tctgtaatac
     7141 agtacactct ctcgatacga ataaacggct cagaaatgag ccgtttattt tttctaccca
     7201 tatccttgaa gcggtgttat aatgccgcgc cctcgatatg gggattttta acgacctgat
     7261 tttcgggtct cagtagtagt tgacattagc ggagcactaa aatgatccaa gaacagacta
     7321 tgctgaacgt cgccgacaac tccggtgcac gtcgcgtaat gtgtatcaag gttctgggtg
     7381 gctcgcaccg tcgctacgca ggcgtaggcg atatcatcaa gatcaccatc aaagaagcaa
     7441 ttccgcgtgg taaggtcaaa aaaggtgatg tgctgaaggc ggtagtggtg cgcaccaaga
     7501 agggtgttcg tcgcccggac ggttctgtca ttcgcttcga tggtaatgct tgtgttcttc
     7561 tgaacaacaa cagcgagcag cctatcggta cgcgtatttt tgggccggta actcgtgagc
     7621 ttcgtagtga gaagttcatg aaaattatct ctctggcacc agaagtactc taaggagcga
     7681 atcatggcag cgaaaatccg tcgtgatgac gaagttatcg tgttaaccgg taaagataaa
     7741 ggtaaacgcg gtaaagttaa gaatgtcctg tcttccggca aggtcattgt tgaaggtatc
     7801 aatctggtta agaaacatca gaagccggtt ccggccctga accaaccggg tggcatcgtt
     7861 gaaaaagaag ccgctattca ggtttccaac gtagcaatct tcaatgcgac aaccggcaag
     7921 gctgaccgtg taggctttag attcgaagac ggtaaaaaag tccgtttctt caagtctaac
     7981 agcgaaacta tcaagtaatt tggagtagta cgatggcgaa actgcatgat tactacaaag
     8041 acgaagtagt taaaaaactc atgactgagt ttaactacaa ttctgtcatg caagtccctc
     8101 gggtcgagaa gatcaccctg aacatgggtg ttggtgaagc gatcgctgac aaaaaactgc
     8161 tggataacgc agcagcagac ctggcagcaa tctccggtca aaaaccgctg atcaccaaag
     8221 cacgcaaatc tgttgcaggc ttcaaaatcc gtcagggcta tccgatcggc tgtaaagtaa
     8281 ctctgcgtgg cgaacgcatg tgggagttct ttgagcgcct gatcactatt gctgtacctc
     8341 gtatccgtga cttccgtggc ctgtccgcta agtctttcga cggtcgtggt aactacagca
     8401 tgggtgtccg tgagcagatc atcttcccag aaatcgacta cgataaagtc gaccgcgttc
     8461 gtggtttgga tattaccatt accactactg cgaaatctga cgaagaaggc cgcgctctgc
     8521 tggctgcctt tgacttcccg ttccgcaagt aaggtagggt tactaaatgg ctaagcaatc
     8581 aatgaaagca cgcgaagtaa aacgcgtagc tttagctgat aaatacttcg cgaaacgcgc
     8641 tgaactgaaa gcgatcatct ctgatgtgaa cgcttccgac gaagatcgtt ggaacgctgt
     8701 tctcaagctg cagactctgc cgcgtgattc cagcccgtct cgtcagcgta accgctgccg
     8761 tcaaacaggt cgtccgcatg gtttcctgcg gaagttcggg ttgagccgta ttaaggtccg
     8821 tgaagccgct atgcgcggtg aaatcccggg tctgaaaaag gctagctggt aattgtcacc
     8881 aattgaatca cgggagtaaa gacagatgag catgcaagat ccgatcgcgg atatgctgac
     8941 ccgtatccgt aacggtcagg ccgcgaacaa agctgcggtc accatgcctt cctccaagct
     9001 gaaagtggca atcgccaacg tgctgaagga agaaggtttt attgaagatt ttaaagttga
     9061 aggcgacacc aagcctgaac tggaacttac tctgaagtat ttccagggca aagctgttgt
     9121 agaaagcatt cagcgtgtca gccgcccagg tctgcgcatc tataaacgta aagatgagct
     9181 gccgaaagtt atggcgggtc tgggtatcgc agttgtttct acctctaaag gtgttatgac
     9241 tgatcgtgca gcgcgccagg ctggtcttgg tggcgaaatt atctgctacg tagcctaatc
     9301 ggaggaaaaa atgtctcgtg ttgctaaagc accggtcgtt gttcctgccg gcgttgacgt
     9361 aaaaatcaac ggtcaggtta ttacgatcaa aggtaaaaac ggcgagctga ctcgtactct
     9421 caacgatgct gttgaagtta aacatgcaga taataccctg accttcggtc cgcgtgatgg
     9481 ttacgcagac ggttgggcac aggctggtac cgcgcgtgcc ctgctgaact caatggttat
     9541 cggtgttacc gaaggcttca ctaagaagct gcagctggtt ggtgtaggtt accgtgcagc
     9601 ggttaaaggc aatgtgatta acctgtctct gggtttctct catcctgttg accatcagct
     9661 gcctgcgggt atcactgctg aatgtccgac tcagactgaa atcgtgctga aaggcgctga
     9721 taagcaggtg atcggccagg ttgcagcgga tctgcgcgcc taccgtcgtc ctgagcctta
     9781 caaaggcaag ggtgttcgtt acgccgacga agtcgtgcgt accaaagagg ctaagaagaa
     9841 gtaaggtaac actatggata agaaatctgc tcgtatccgt cgtgcgaccc gcgcacgccg
     9901 caagctccag gagctgggcg caactcgcct ggtggtacat cgtaccccgc gtcacattta
     9961 cgcacaggta attgcaccga acggttctga agttctggta gctgcttcta ctgtagaaaa
    10021 agctatcgct gaacaactga agtacaccgg taacaaagac gcggctgcag ctgtgggtaa
    10081 agctgtcgct gaacgcgctc tggaaaaagg catcaaagat gtatcctttg accgttccgg
    10141 gttccaatat catggtcgtg tccaggcact ggcagatgct gcccgtgaag ctggccttca
    10201 gttctaaggt agaggtgtaa gatggctcac atcgaaaaac aagctggcga actgcaggaa
    10261 aagctgatcg cggtaaaccg cgtatctaaa accgttaaag gtggtcgtat tttctccttc
    10321 acagctctga ctgtagttgg cgatggtaac ggtcgcgttg gttttggtta cggtaaagcg
    10381 cgtgaagttc cagcagcgat ccagaaagcg atggaaaaag cccgtcgcaa tatgattaac
    10441 gtcgcgctga ataacggcac tctgcaacac cctgttaaag gtgttcacac gggttctcgc
    10501 gtattcatgc agccggcttc cgagggtacc ggtatcatcg ccggtggtgc aatgcgcgcc
    10561 gttctggaag tcgctggggt tcataacgtt ctggctaaag cgtatggttc caccaacccg
    10621 attaacgtgg ttcgtgcaac tatcgatggc ctggaaaata tgaattctcc agaaatggtc
    10681 gctgccaagc gtggtaaatc cgttgaagaa attctgggga aataaaccat ggcaaagact
    10741 attaaaatta ctcaaacccg cagtgcaatc ggtcgtctgc cgaaacacaa ggcaacgctg
    10801 cttggcctgg gtctgcgtcg tattggtcac accgtagagc gcgaggatac tcctgctatt
    10861 cgcggtatga tcaacgcggt ttccttcatg gttaaagttg aggagtaaga gatgcgttta
    10921 aatactctgt ctccggccga aggctccaaa aaggcgggta aacgcctggg tcgtggtatc
    10981 ggttctggcc tcggtaaaac cggtggtcgt ggtcacaaag gtcagaaatc tcgttctggc
    11041 ggtggcgtac gtcgcggttt cgagggtggt cagatgcctc tgtaccgtcg tctgccgaaa
    11101 ttcggcttca cttctcgtaa agcagcgatt acagccgaag ttcgtctgtc tgacctggct
    11161 aaagtagaag gcggtgttgt agacctgaac acgctgaaag cggctaacat tatcggtatc
    11221 cagatcgagt tcgcgaaagt gatcctggct ggcgaagtaa cgactccggt aactgttcgt
    11281 ggcctgcgtg ttactaaagg cgctcgtgct gctatcgaag ctgctggcgg taaaatcgag
    11341 gaataagtag cagatggcta aacaaccggg attagatttt caaagtgcca aaggtggctt
    11401 aggcgagctg aaacgcagac tgctgtttgt tatcggtgcg ctgattgtgt tccgtattgg
    11461 ctcttttatt ccgatccctg gtattgatgc cgctgtactt gccaaactgc ttgagcaaca
    11521 gcgaggcacc atcattgaga tgtttaacat gttctctggt ggtgctctca gccgtgcttc
    11581 tatctttgct ctggggatca tgccgtatat ttcggcgtcg atcattatcc agctactgac
    11641 ggtggttcat ccaacgttgg cagaaattaa gaaagaaggg gagtctggtc gtcgtaagat
    11701 cagccagtac acccgctacg gtactctggt gctggcaata ttccagtcga tcggtattgc
    11761 tactggtctg ccgaatatgc ctggtatgca aggcctggtg attaacccgg gctttgcatt
    11821 ctacttcacc gctgttgtaa gtctggtcac aggaaccatg ttcctgatgt ggttgggcga
    11881 acagatcact gaacgaggta tcggcaacgg tatttcaatc attatcttcg ccggtattgt
    11941 cgcgggactc ccgccagcca ttgcccatac tatcgagcaa gcgcgtcaag gcgacctgca
    12001 cttcctcgtg ttgctgttgg ttgcagtatt agtatttgca gtgacgttct ttgttgtatt
    12061 tgttgagcgt ggtcaacgcc gcattgtggt aaactacgcg aaacgtcagc aaggtcgtcg
    12121 tgtctatgct gcacagagca cacatttacc gctgaaagtg aatatggcgg gggtaatccc
    12181 ggcaatcttc gcttccagta ttattctgtt cccggcgacc atcgcgtcat ggttcggggg
    12241 cggtactggt tggaactggc tgacaacaat ttcgctgtat ttgcagcctg ggcaaccgct
    12301 ttatgtgtta ctctatgcgt ctgcaatcat cttcttctgt ttcttctaca cggcgttggt
    12361 tttcaacccg cgtgaaacag cagataacct gaagaagtcc ggtgcatttg taccaggaat
    12421 tcgtccggga gagcaaacgg cgaagtatat cgataaagta atgacccgcc tgaccttggt
    12481 tggtgcgctg tacattacct ttatctgcct gatcccggag ttcatgcgtg atgcaatgaa
    12541 agtaccgttc tacttcggtg ggacctcact gcttatcgtt gttgtcgtga ttatggactt
    12601 tatggctcaa gtgcaaactc tgatgatgtc cagtcagtat gagtctgcat tgaagaaggc
    12661 gaacctgaaa ggctacggcc gataattggt cgcccgagaa gttacggaga gtaaaaatga
    12721 aagttcgtgc ttccgtcaag aaattatgcc gtaactgcaa aatcgttaag cgtgatggtg
    12781 tcatccgtgt gatttgcagt gccgagccga agcataaaca gcgccaaggc tgattttttc
    12841 gcatattttt cttgcaaagt tgggttgagc tggctagatt agccagccaa tcttttgtat
    12901 gtctgtgcgt ttccatttga gtatcctgaa aacgggcttt tcagcatgga acgtacatat
    12961 taaatagtag gagtgcatag tggcccgtat agcaggcatt aacattcctg atcataagca
    13021 tgccgtaatc gcattaactt cgatttatgg cgtcggcaag acccgttcta aagccatcct
    13081 ggctgcagcg ggtatcgctg aagatgttaa gatcagtgag ctgtctgaag gacaaatcga
    13141 cacgctgcgt gacgaagttg ccaaatttgt cgttgaaggt gatctgcgcc gtgaaatcag
    13201 catgagcatc aagcgcctga tggatcttgg ttgctatcgc ggtttgcgtc atcgtcgtgg
    13261 tctcccggtt cgcggtcagc gtaccaagac caacgcacgt acccgtaagg gtccgcgcaa
    13321 accgatcaag aaataatcgg ggtgattgaa taatggcaaa ggcaccaatt cgtgcacgta
    13381 aacgtgtaag aaaacaagtc tctgacggcg tggctcatat ccatgcttct ttcaacaaca
    13441 ccatcgtgac tatcactgat cgtcagggta acgcgttggg ttgggcaaca gccggtggtt
    13501 ccggtttccg tggttctcgc aaatccactc cgtttgcagc acaggttgca gcagagcgtt
    13561 gcgctgacgt cgtgaaagaa tacggcatca agaatctgga agttatggtt aaaggtccgg
    13621 gtccaggccg cgaatctact attcgtgctc tgaacgccgc aggtttccgc atcactaaca
    13681 ttactgatgt gactccgatc cctcataacg gttgtcgtcc gccgaaaaaa cgtcgcgtat
    13741 aacgcctcgt tttccaggtt tgttggagaa agaaaatggc aagatatttg ggtcctaagc
    13801 tcaagctgag ccgtcgtgag ggcaccgact tattccttaa gtctggcgtt cgcgcgatcg
    13861 ataccaagtg taaaattgaa caagctcctg gccagcacgg tgcgcgtaaa ccgcgtctgt
    13921 ctgactatgg tgtgcagttg cgtgaaaagc aaaaagttcg ccgtatctat ggtgtgctgg
    13981 agcgtcagtt ccgtaactac tacaaagaag cagcacgtct gaaaggcaac accggtgaaa
    14041 acctgttggc tctgctggaa ggtcgtctgg acaacgttgt ataccgtatg ggcttcggtg
    14101 ccactcgtgc agaagcacgt cagctggtta gccataaagc aattatggta aacggtcgtg
    14161 ttgttaacat cgcttcttat caggttagtc cgaatgacgt tgtaagcatt cgtgagaaag
    14221 cgaagaagca gtctcgcgtg aaagccgctc tggagctggc tgagcagcgt gaaaagccaa
    14281 cctggctgga agttgatgct ggcaagatgg aaggtacgtt taagcgtaag ccggagcgtt
    14341 ctgatctgtc tgcggacatt aacgaacacc tgatcgtcga gctttactcc aagtaaagct
    14401 tagtaccaaa gagaggacac aatgcagggt tctgtgacag agtttctaaa accgcgcctg
    14461 gttgatatcg agcaagtgag ttcgacgcac gccaaggtga cccttgagcc tttagagcgt
    14521 ggctttggcc atactctggg taacgcactg cgccgtattc tgctctcatc gatgccgggt
    14581 tgcgcggtga ccgaggttga gattgatggt gtactacatg agtacagcac caaagaaggc
    14641 gttcaggaag atatcctgga aatcctgctc aacctgaaag ggctggcggt gagagttcag
    14701 ggcaaagatg aagttattct taccttgaat aaatctggca ttggccctgt gactgcagcc
    14761 gatatcaccc acgacggtga tgtcgaaatc gtcaagccgc agcacgtgat ctgccacctg
    14821 accgatgaga acgcgtctat tagcatgcgt atcaaagttc agcgcggtcg tggttatgtg
    14881 ccggcttcta cccgaattca ttcggaagaa gatgagcgcc caatcggccg tctgctggtc
    14941 gacgcatgct acagccctgt ggagcgtatt gcctacaatg ttgaagcagc gcgtgtagaa
    15001 cagcgtaccg acctggacaa gctggtcatc gaaatggaaa ccaacggcac aatcgatcct
    15061 gaagaggcga ttcgtcgtgc ggcaaccatt ctggctgaac aactggaagc tttcgttgac
    15121 ttacgtgatg tacgtcagcc tgaagtgaaa gaagagaaac cagagttcga tccgatcctg
    15181 ctgcgccctg ttgacgatct ggaattgact gtccgctctg ctaactgcct taaagcagaa
    15241 gctatccact atatcggtga tctggtacag cgtaccgagg ttgagctcct taaaacgcct
    15301 aaccttggta aaaaatctct tactgagatt aaagacgtgc tggcttcccg tggactgtct
    15361 ctgggcatgc gcctggaaaa ctggccaccg gcaagcatcg ctgacgagta accggatcac
    15421 aggttaaggt tttactgaga aggataaggt catgcgccat cgtaagagtg gtcgtcaact
    15481 gaaccgcaac agcagccatc gccaggctat gttccgcaat atggcaggtt cactggttcg
    15541 tcatgaaatc atcaagacga ctctgcctaa agcgaaagag ctgcgccgcg tagttgagcc
    15601 gctgattact cttgccaaga ctgatagcgt tgctaatcgt cgtctggcat tcgcccgtac
    15661 tcgtgataac gagatcgtgg caaaactgtt taacgaactg ggcccgcgtt tcgcgagccg
    15721 tgccggtggt tacactcgta ttctgaagtg tggcttccgt gcaggcgaca atgcgccgat
    15781 ggcttacatc gagctggttg atcgttcaga gaaagcagaa gctgctgcag agtaatctga
    15841 agcaacgtaa aaaaacccgc cccggcgggt ttttttatac ccgtagcatc cccacttatc
    15901 tacaatagct gtactctttt tgttcatccc ctggagtatt tatgtggtta cttgaccagt
    15961 gggcagagcg tcatatagca gaagcgcaag cgaaaggtga gtttgataac ctggtaggta
    16021 gcggcgaacc attgatactg gatgatgatt ctcatgtgcc accggaatta cgtgcggggt
    16081 atcgcttgct gaagaatgct ggttgcttac cgccagaact tgagcatagg agggaagcaa
    16141 tacagcttct ggatattctc aaaggtatcc gtcatgatga tccgcaatat caagaggtta
    16201 gtcgtcgatt gtcattactg gaattgaagc tgcgacaagc tggattgagt accgattttt
    16261 tacgcggcga ttatgctgac aagttgttga acaaaatcaa cgataactag tggagtatgt
    16321 atgtatcgca ttggtgagct ggcaaaaatg gcggaagtaa cacccgacac gattcgttat
    16381 tacgaaaagc agcagatgat ggagcatgaa gtacgcaccg aaggtgggtt tcgcctgtat
    16441 accgaaagcg atctccagcg attgaaattt atccgccatg ccagacaact aggtttcagt
    16501 ctggagtcga tccgcgagtt gctgtcgatc cgtatcgatc ctgaacacca tacctgtcag
    16561 gagtcaaaag gcattgtgca ggaaagattg caggaagtcg aagctcgtat agccgagttg
    16621 cagagtatgc agcgttcctt gcaacgcctt aatgatacct gttgtgggac cgcccatagc
    16681 agtgtttatt gctcgattct tgaagctctt gaacaagggg cgagtggcgt taagagtggc
    16741 tgttgatttt ttgcactggc aggattacac tcgcgccgtt aaataaccaa ttggagtttt
    16801 tatgagccga tatcagcata ctaaagggca gataaaggat aatgcgatag aagcattact
    16861 acatgatccc ttattccgac agcgagtaga gaaaaataag aaagggaaag gcagttacat
    16921 gcgaaaagga aaacatggca atcgggggaa ctgggaggcc agtggcaaga aagtaaatca
    16981 cttttttacc actggccttc tgctttcaga cgcttgttaa gagcggttat tctgctcttt
    17041 cagcaaatca cgaatttctg tcagtaatac ttcttcttta gttggtgcag gtgttgctgc
    17101 tgctggttct tcttttttcc gattcagttt gttgattagc ttaatcgcca taaagatggc
    17161 aaaggccact atcagaaaat caaagacgtt ttgaatgaag acaccgtaat gcatcacaac
    17221 agcagggata tccccttggg cctcgcgtag cgtgacagca aactgtttaa agtcgatccc
    17281 gccaattaat aagcccagag gtggcatgat gatatcggca accagtgaag atacaatctt
    17341 cccgaatgcc gctccgataa tgacacccac tgccaaatcc accacgttcc cgcgcatcgc
    17401 aaattcgcga aattctttaa taatgctcat gttattctcc ctatgaagcc gacaaccata
    17461 agtctaacaa atgttaagcc attttcctgc caggaatgga tttaattaat ccttaataa
//
LOCUS       DAOFSV010000089        17276 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.089, whole genome shotgun sequence.
ACCESSION   DAOFSV010000089 DAOFSV010000000
VERSION     DAOFSV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.089"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            54..959
                     /gene="yijE"
                     /locus_tag="QBH27_003136"
     CDS             54..959
                     /gene="yijE"
                     /locus_tag="QBH27_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDQ7015417.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(986..1603)
                     /locus_tag="QBH27_003137"
     CDS             complement(986..1603)
                     /locus_tag="QBH27_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDQ7015418.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(1879..2982)
                     /gene="gldA"
                     /locus_tag="QBH27_003138"
     CDS             complement(1879..2982)
                     /gene="gldA"
                     /locus_tag="QBH27_003138"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDQ7015419.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(2993..3655)
                     /gene="fsa"
                     /locus_tag="QBH27_003139"
     CDS             complement(2993..3655)
                     /gene="fsa"
                     /locus_tag="QBH27_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDQ7015420.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(3667..6168)
                     /gene="ptsP"
                     /locus_tag="QBH27_003140"
     CDS             complement(3667..6168)
                     /gene="ptsP"
                     /locus_tag="QBH27_003140"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ7015421.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            6477..7556
                     /locus_tag="QBH27_003141"
     CDS             6477..7556
                     /locus_tag="QBH27_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDQ7015422.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            7571..7891
                     /locus_tag="QBH27_003142"
     CDS             7571..7891
                     /locus_tag="QBH27_003142"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDQ7015423.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            7942..10239
                     /locus_tag="QBH27_003143"
     CDS             7942..10239
                     /locus_tag="QBH27_003143"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDQ7015424.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVLLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGAVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            10205..11083
                     /locus_tag="QBH27_003144"
     CDS             10205..11083
                     /locus_tag="QBH27_003144"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDQ7015425.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            11085..11426
                     /locus_tag="QBH27_003145"
     CDS             11085..11426
                     /locus_tag="QBH27_003145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDQ7015426.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11413..12264)
                     /locus_tag="QBH27_003146"
     CDS             complement(11413..12264)
                     /locus_tag="QBH27_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7015427.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(12490..14223)
                     /locus_tag="QBH27_003147"
     CDS             complement(12490..14223)
                     /locus_tag="QBH27_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDQ7015428.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(14406..17057)
                     /gene="ppc"
                     /locus_tag="QBH27_003148"
     CDS             complement(14406..17057)
                     /gene="ppc"
                     /locus_tag="QBH27_003148"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDQ7015429.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
BASE COUNT         3862 a         4441 c         4763 g         4210 t
ORIGIN      
        1 agtttcttta ccggcgtata gtgtccacag gaaaactaca cactggatct ctcatgtctg
       61 ccgcaggaaa gagcaaccca ctggcaatca gtggcctggt tgtgctcaca cttatctgga
      121 gttatagctg gattttcatg aagcaagtca ccagctacat cggtgccttc gacttcaccg
      181 ccttacgctg cattttcggc gctctcgttt tattcatcgt tcttttatta cgtggtcgcg
      241 gaatgcgccc gacgccgttt aaatacacct tagccattgc cctgttacaa acctgcggga
      301 tggttggcct ggcacagtgg gcgctggtca gcggcggagc ggggaaggtg gcaatcctga
      361 gctataccat gccgttctgg gtggtgattt tcgccgcgtt gtttctcggt gaacgcctgc
      421 gacgtgggca atatttcgcg atactgattg ccgctttcgg cttatttttg gtgttgcagc
      481 cgtggcaact cgatttctct tcgatgaaaa gtgccatgct ggcaatcctc tccggtgtga
      541 gttggggggc gagcgcgatt gttgctaaac gtctgtatgc ccgccatccg cgcgtggatt
      601 tattgtcgct aacatcctgg cagatgctgt atgcggcgct ggtgatgagc gtggtcgctt
      661 tactggtgcc gcaacgtgaa attgactggc aacccaccgt gttctgggcg ctggcctaca
      721 gtgcgattct ggcgacagcg ctggcgtgga gcttatggtt gtttgtattg aaaaacttgc
      781 ctgccagtat tgccagctta agcacactgg ccgttcccgt ttgcggcgta ctcttttcct
      841 ggtggctgct cggtgagaat ccgggggcag ttgaaggtag cggtattgtg ctgattgtac
      901 tggcactggc gctggtgagc cgtaagaaaa aagaagccgt cagtgtaaaa aggatctgaa
      961 ttttttcttc atgtggggag atctcttatt tagcaaaata ccgatagtgc cccaccatcc
     1021 gccagctaaa cagtacatct tcttcctgcg cgcctgcgcc aatgttatgt atcaccagcg
     1081 gcgtaccgtc gcgggcgaag ccatctgaca ccaccccaat atgtgccagc ccgttatcta
     1141 atcgccagga gacaatatcg cccgcttgat agtcactggg gttcttgctg gtggggcgtg
     1201 ttttatcgtg gcgggtaaac caggtttcca gattaggcac ccgacggtga tcgatgttgc
     1261 tgtccgggcg ctttaactgc catttttgcg ggtacgcagc aaaattcttc gccatatctt
     1321 cgtgcaccag tttctgcaaa tcgaccttct ggctgcgcaa tgcacggatc accacatcgg
     1381 aacatacgcc gcgttcttgc gggacatcac cgccaggata ggtaagctgc acatacgccg
     1441 gatcgtaaaa tagcgtgctg ccgatttgct gtctggcgcc gtctgcgatg gcaagattgg
     1501 tgttggcctg gatttgtacc acggttggtg gaacggcggg agattttaag gagtaactgg
     1561 taaataccgt cagaagactg aataacgcca gtgaagcttt catctgtttc gtccgtgaaa
     1621 ataagagtca cagattcgac cttccgggct aacgattgtt ctgtggagaa aatgtccaga
     1681 agcggataaa aaaacacccg aacaacgtgg aagtttcaga ttactggcac agcgtgccgg
     1741 atgcggcgtg aacgccttat ccggtctaca aaagtacgta aatttactat attgcagaga
     1801 ttgcgtaggc ctgataagcg tagcgcatca ggcaattttg cgttcaaact cccggacaag
     1861 ccgggagttt ggagtagatt attcccactc ttgcaggaaa cgctgaccat actggtcggc
     1921 taccagcagt gcggcgtaaa cctgatctgg cgtcgcgccg ccaggcatgt tgtggatggt
     1981 ttcaccttct gcacatgccg cttctgccac aattcgcatt tttgccggga catcttcttt
     2041 aatatccagt tgagcgagag ttattggcaa acctaccgca tggctaagcg cagctacggt
     2101 ttcgatttcc tccaccggcg cgttttccag aaccagctgc gtcagcgtac cgaatgccac
     2161 tttttcacca tgataatagt gatgcgcgtc cgggatagcg gtcaggccgt tatgtaccgc
     2221 gtgtgccgcc gccagcccgc cgctttcaaa accaacgcca ctcaaatagg tgttcgcttc
     2281 aatcacgcgc tccagcgccg gagtcactac atgctgttcg gcggcaagca tcgctttttc
     2341 gccttcttcc agcagggtgt tgtagcacag ttcagccagt gccagcgctg cctgagtgca
     2401 cttgccaccc gccatggtgg tcgcgccgct acgggaacag gcacgcgctt caaaccaggt
     2461 tgccagcgca tcgccaatac ccgccgctaa cagacgcgcg ggcgcgccag cgacgatttt
     2521 ggtgtcgaca atgaccatat tcgggttatt tggcaacagc agatagcggt caaactcacc
     2581 ctcatcggtg tagataacag acaatgcgct gcacggtgcg tcggtagaag cgatagtcgg
     2641 tgcgatcgct accggaacac ccatgaaatg tgccagtgct ttggcagtat cgagggtttt
     2701 tccgccacca ataccgagaa ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg
     2761 gtcgatctca ttttgcgaac attcaccgcc aaacggcgca atttctacta ccagtccagc
     2821 atctttaaag cttttctcga cagtggattg agcaaaacct aaaacaaatt tgtcacccac
     2881 cactaaccag cgttccgcca gtggcttcag gtactcgccc agacgattaa ttacatctgc
     2941 gccctggatg tatttacctg gtgattgaat aatgcggtcc ataattactc ctttagagat
     3001 gagtagtgcc aaatgcggca ttccagtcgt gctcgaactt ctctatagct gactctaccg
     3061 caggggtgtt aagcatttgt tgcgctacat ctaagggcag tgtgatggat tcacatcctg
     3121 ccagcaaaca atccagcgcc tgacgtggtg ttttaaagct ggcagccagc accatgcttt
     3181 ctggcgcatg catttccagt aacgcttgca actcctgtac agtacgaatg ccgtcaccgc
     3241 cctgggcatc tacgcggtta acgtatggag caacgtattt ggctccagcc agcgccgcca
     3301 gtaatccttg cgcggcgctg tacactgcgg ttcccagcgt agtaatgcct tctttcttca
     3361 gcattttaat tgctgccaga ccttcagagg ttaccggaat tttcaccaca atgcccggaa
     3421 tagcgtcgcg caggcgtttc gcttctttca ccataccctg cgcgtcgcgg ctcatggtct
     3481 gagcaaacag aatgccctca tcaccgatcg ctttttgcag gcgcggcagc acttcccaga
     3541 tggactcctt gctggcagcg ataatgctcg ggttagttgt caccccggca atcgggaata
     3601 tgcgtgccag acgttcgact tctgcgacgt tagcggtgtc cagatacagt tccatgatgt
     3661 ttccttttac agttccagtt catgttgcag caggctggcg atagcgtcgg cagaggcggc
     3721 gttaaccagc gcattacgga attcttcgtg catgatgcga cgagcgaggc gcgagaaaat
     3781 gcgcatatgt tgatcgcccg cggcgtgttt gttcagggtt aacataatga tgaattgcgc
     3841 ttcatcatcg ccccagcgca ctggcgcttg cagacgcgct acgctgatgg tggattgctc
     3901 aatgtgttcc gatttactgt ggggaatggc gaaactaaag cccagtccgg tagagaaaac
     3961 ggcctcgcgc gcccacaagt cggcttccag tttacgcgga tagcggcagc ggcccgccag
     4021 cagcaggtta tcggtcatgc ctttgagcac ttcttctttg ctgcgccagt cgctttccag
     4081 tgtgatgcac tcggcggtga ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag
     4141 caggtgttcc acttccagcg aagtacggca ggccattgcc tggttgagca actggcggca
     4201 ctcacggcta tcaagttgcg ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat
     4261 gctgagttca tccagcccta aaccgaccag caacggcagc acggaacctt tcgctcccag
     4321 ttcaccgcac agaccaatcc atttgccctg gcgatgcacc gcctgcacgg cgtaatcgag
     4381 cgcccgcaag aaggccggat tcaggctgtt gtagtgacga gtcaccttag cgttatcgcg
     4441 atccactgcc agtagatatt gcgtcaggtc attactacca atactaaaga aatcaatctc
     4501 ttcgcagcat tgatcgatga tgaacatcac cgacggcact tccagcatga tgccgagctg
     4561 gatcttctca tcaaacggaa tgtgttcgtt acgcagttgc tgtttggctt ccgccagttt
     4621 ttctttcacc cataagatct cttccattga ggagatcatc gggatcatga ttttcaggct
     4681 gccgtgagca gaggcgcgca ggatcgatcg tagttgtgtg gtaaacaacg acgcgtattc
     4741 ttcataaata cgcaccgcgc gataaccgag gaacgggtta gcttctgcgg gaatgttcag
     4801 ataatcaacg ggtttgtcac caccaatgtc catagtgcgc acaataatgc tgcgtccgtt
     4861 ggcagactcc agcgcctgac aaaagatgtt gtacagctcg ctttcacccg gtgcactggt
     4921 gcgatccata tagagcattt cagtgcggaa caaaccaacg ccttccgcac cattgccgaa
     4981 tgcggcctgc gcttccacgg agtgagcgat gttagcggca atttcaatgc ggataccgtc
     5041 agcggtacgc gcttgctggg tcagccagac acgctgttgc tcacgcagag cgtcctgtac
     5101 gcgggcttct tgctgataat aacgggccac ggattcccct ggctcaacca caatcgcccc
     5161 tgcgttgccg tcgatataaa tcgtctgatg ctgccacgga gtaagggcat caatatccac
     5221 acctaccagc gtcggaatgt tgaacgaacg ggcaaggatc accgtatgtg atgtggtgcc
     5281 gccacttttc agtaacagac ctttgagatg atttttatcc agttcgagga actggctggg
     5341 ggtcagttca tcagccatac aaatggcggg ctgcgtcagt ttgcccggtg ccgggaagcg
     5401 ttgctcaccg tagatttgct ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg
     5461 ttcttgcagg taactgctgc tggaacgggc gaactcttcg caaaagtgat tcgcactggc
     5521 aacaatcgct tcggcgcagc ttaatccggc gctgacacct gccagtaaat gttcgcgcag
     5581 ggaagtatcg ccagccaggg atcggtgcgc ttccagaatc gcgctggtag caccgtcgct
     5641 atccagcaga cgaaactcta tgtttttcag taccagcgtc agaccgtttt ccagtgcgga
     5701 ttgttcggcg tcaacgcctt tggctgcggg aagattaccc agcgcattga gatctaaaga
     5761 agagatcggc gtcagaatgc cgcctgcgct accgctgcac acggtgcgag cgcggataat
     5821 ttgcggattc agattggtca gtgaaaccgg cagtggttcc agttcgtcag attttacttc
     5881 cgccagcggt gcgtcgcagt gggggaattc atcgcgcagc cattggctta aacgctggtg
     5941 cgcttcctgt tcgtcggccc cggaaatcag taactggcag ttatcgccca ccagcgtatc
     6001 ggtgccaatc agcgcaaggg cgcttttggc gttgcccttg cggtcagtgc gcaggttatg
     6061 ccactcaatt tgtgatgaaa aagtattaca cagcgtttca acgtggcttg ccggacgcgc
     6121 atggacgccg ttaggtagct cacaaataaa ttccacaatc agggccattg cctttctccc
     6181 ggtacggttt tctgtttaca ggataagagc gcacggcaac ggcctgccat gtgacaaatc
     6241 tgccaaaagc tggacaaatg taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc
     6301 gttttgcggc gtacttccgg attatcgcaa gttatgaggc ggatcgcatt tttgtactga
     6361 tattacaaaa gtccagtaaa tggccttttt atccactgtt tgctccgcct gcgattgcct
     6421 attgtttctt ccatcaaaaa tatggacatg ggctttcgca agcccgggag cagcttatga
     6481 atgagttggt gcagatcctg aaaaataccc gtcagcattt aatgacgggc gtttcacaca
     6541 tgattccctt cgtggtatcg ggcggtattt tgctggcggt ttccgtcatg ttgtatggca
     6601 aaggcgcagt gccggatgcc gtagccgatc cgaatctgaa aaaactgttt gatatcggcg
     6661 ttgcaggttt gacgctgatg gtgcctttcc tcgccgctta catcggttat tccattgcag
     6721 aacgttctgc gctggctcca tgcgctatcg gtgcctgggt tggtaacagc tttggcgcgg
     6781 gcttctttgg tgcgctgatc gccgggatta tcggcggcat cgtggtgcat tacctgaaga
     6841 aaattccagt gcataaagtt ctgcgctcgg taatgccaat cttcatcatt ccaatcgtcg
     6901 gcacactgat taccgctggc gtcatgatgt gggggctggg cgagccggtg ggggcgttga
     6961 ccaacagcct gacccagtgg cttcagggga tgcagcaggg cagcattgtt atgctggcgg
     7021 tgatcatggg tctgatgctg gcgttcgata tgggcggccc ggttaacaaa gtggcctatg
     7081 ccttcatgct gatttgcgtt gctcagggtg tttataccgt ggtggctatt gccgctgttg
     7141 ggatttgtgt tccaccgctg gggatggggc tggcgacgct gattggtcgt aaaaatttct
     7201 ccgcagaaga gcgcgaaacc ggcaaggcgg cactggtgat gggctgtgtt ggcgttactg
     7261 aaggggcgat tcctttcgcc gctgccgatc cgctgcgtgt cattccttcc atcatggtcg
     7321 gttcagtttg tggtgcggta actgcggcgc tggtcggtgc gcagtgctat gcaggctggg
     7381 gtggtctgat tgtgctgccg gtggttgaag gtaagttggg ttacatcgcg gcagtggctg
     7441 tcggggcggt tgtgacggca gtctgcgtga acgtgctgaa aagtctggcg cgtaaaaatg
     7501 ggtcttcaac tgaagagaag gaagacgacc tggatctgga ttttgaaatt aattaattga
     7561 ggaacgagcc atgacgaaaa ttatcgcagt aaccgcatgt ccttcaggtg ttgcccatac
     7621 ttatatggcg gcagaggcgc tggaaagcgc cgcgaaagcg aaaggctggg aagtaaaagt
     7681 agaaacccag gggtcgattg gtctggaaaa tgaactgact gcggaagacg tggcaagcgc
     7741 cgatatggtg attttgacca aagatatcgg catcaaattt gaagagcgtt ttgctggcaa
     7801 gactatcgtg cgtgtcaaca tcagcgacgc ggtaaagcgt gctgacgcca ttatgagcaa
     7861 aattgaagct cacctggcac aaaccgctta agttcttatc ccgcccgcaa gggcgggttc
     7921 gctttcccac aggagttcct catgacgaat cgtatctctc gcctcaaaac tgcactgttt
     7981 gccaataccc gtgaaatctc gctggagcgg gcgttgcttt ataccgccag ccatcggcaa
     8041 accgaagggg agccggtgat aatgcgtcgg gcgaaagcaa cagcgtatat ccttgaacac
     8101 gttgaaattt cgattcgtga tgaagaactg attgccggta accgcaccgt aaaaccacgc
     8161 gccgggatta tgtcgccgga aatggaccct tactggctgc tgaaagaact ggatcaattc
     8221 ccgacgcgtc cgcaggaccg ctttgctatc agcgaagaag ataaacgtat ctaccgcgaa
     8281 gagttgttcc cgtactggga aaaacgttcg atgaaagatt tcatcaacgg gcagatgacg
     8341 gatgaagtaa aagccgcgac cagcacgcag attttcagca tcaaccagac ggataaaggc
     8401 caggggcaca ttattattga ttacccgcgc ctgctgaatc acgggctggg ggagctggtg
     8461 gcacagatgc agcaacattg tcagcaacag ccggagaatc acttttatca ggcagcgctg
     8521 ttactgctgg aagcctcgca gaaacatatt ttgcgttacg ccgaactggc ggaaacgatg
     8581 gcggcaagct gcactgatgg cccgcgtcgc gaagaattgc tgactattgc ggagatctcc
     8641 cgccataacg ccgaacataa gccgcagacg ttctggcagg cgtgccagtt attctggtac
     8701 atgaacatca ttctgcaata cgaatccaac gccagttcgc tatcgttggg gcgcttcgac
     8761 cagtatatgt tgccgttcta tcagacatca ttaacccagg gcgaagatcc ggcattcctg
     8821 aaagaactgc tcgaatcttt atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc
     8881 agtagcgcgc gttatttcgc cggtttccct accggctata ccgcactgct cggcgggtta
     8941 accgagaacg gacgtagcgc ggtgaacgtg ctttcgttcc tttgccttga cgcttatcaa
     9001 agcgtgcaat taccgcaacc gaacctcggc gtgcgcacta acgccttgat cgacacgccg
     9061 ttcctgatga aaaccgcaga aaccattcgc ctcggcaccg gtattccgca aatctttaac
     9121 gatgaagtgg tagtgccagc gttcctcaac cgtggcgttt tgctggaaga tgcgcgcgac
     9181 tattccgtag tgggctgtgt ggaattatct attcccggca gaacctacgg cttgcatgac
     9241 atcgcgatgt ttaacctgct gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaac
     9301 gctgcgctga cttatgaagg tttactggaa cagatccgcg ccaagatcag ccactacatc
     9361 accctgatgg ttgagggcag caatatttgt gatatcggcc atcgcgactg ggcacctgta
     9421 ccgctactct cgtcttttat cagcgattgt ctggaaaaag gtcgcgatat taccgatggc
     9481 ggcgcgcgtt ataacttctc cggcgtacag gggatcggta tcgccaacct gagcgattct
     9541 ctccatgcgt tgaaagggat gatttttgat caacagcgtt taagttttga cgaattgctg
     9601 tcggtattaa aagccaactt cgccacgcca gaaggcgaaa aagtccgcgc tcgcttaatt
     9661 aaccgcttcg agaaatacgg taacgatatc gacgaggtgg ataacattag cgccgaactg
     9721 ttacgccact actgcaaaga agtggaaaaa taccagaacc cgcgcggcgg ctacttcacg
     9781 ccgggatcgt ataccgtttc tgctcatgtc ccgttgggag cggtggttgg cgcgacgcca
     9841 gacggtcgct ttgccggaga gcaactggca gacggtggct tgtcacccat gctgggtcag
     9901 gacgcacaag gaccaacggc ggtactgaaa tcagtcagta agctcgataa cacactgctt
     9961 tctaacggta cattgctgaa cgtgaaattc actccggcga ccctcgaagg tgaagcggga
    10021 ttacgcaaac tggccgactt cttacgggcg tttacccagc ttaagttgca gcatattcag
    10081 tttaacgtgg tgaacgccga cacgttacgg gaagcgcaac agcgcccaca agattatgcc
    10141 gggctggtgg tgcgcgttgc cggatacagc gccttctttg tcgaactgtc gaaggagatc
    10201 caggatgaca tcatccgccg gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga
    10261 tgatgtggcg cgcattttca acattcagcg ttattcactg aatgacggcg agggcattcg
    10321 tacggtggtc ttttttaaag gctgtccgca tctttgccca tggtgtgcta atccggagtc
    10381 gatctccggc aaaatccaga cggtgcgcag agaggcgaaa tgtctgcact gcgcgaaatg
    10441 tttgcgtgat gcggatgaat gcccctccgg ggcatttgaa cggattggcc gcgatatcag
    10501 ccttgacgcg ctggaacggg aagtgatgaa agatgacatt ttctttcgca cgtccggcgg
    10561 cggcgtcacg ctttctggcg gcgaagtgtt aatgcaggcg gagtttgcga cccgtttttt
    10621 acagcgactg cggctatggg gtgtgtcatg cgccatcgaa actgccggag acgcgccagc
    10681 cagcaagctg ttaccgctgg cgaaattgtg cgatgaagtg ttgttcgatt taaaaattat
    10741 ggatgcggct caggcgcggg atgtggtgaa gatgaacctg caacgcgtgc tggagaatct
    10801 gcgtttgctg gtgagtgagg gtgtcaacgt gatcccgcgt ttaccgctga tccctggttt
    10861 cacgctcagc cgggagaata tgcagcaggc gctggacgtg ctgatcccgc tgaatatcaa
    10921 gcagatccat ctgttaccgt ttcatcagta cggcgagccg aaataccgcc tgctggggaa
    10981 aacatggtcg atgaaagagg ttgctgcgcc gtcgtcagcc gatgtggcaa cgatgcgtga
    11041 aatggcagaa cgggccggat ttcaggttac cgtgggaggt taaaatggca tatctggtgg
    11101 cagtaaccgc ctgcgtaagc ggcgtggcgc atacttatat ggcggcggaa cggctggaaa
    11161 agttgtgcca gttagagaag tggggcgtca gcattgaaac tcagggcgcg ttgggaacgg
    11221 agaatcgttt agcggacgag gatatccgcc gggcggatgt tgcgctgtta attacggata
    11281 tcgagcttgc cggtgctgag cgatttgaac attgtcgcta cgtgcaatgc agcatctacg
    11341 ctttcctgcg tgagccgcag agggtgatga gcgcagtgcg caaagtgctt tctgcgccgc
    11401 agcaaaccca tcttattctg gagtagtcgg tttttctgtc agttggctgt ggtactgccg
    11461 acgatattcc gacggcgagc gttcggtatt tttacgaaac agacggcaga agtagttgct
    11521 gtcgacaaag ccgcaggcgt gcgccacttc ttttaccttc aggtcatagc ctttcaataa
    11581 tgtcttagcg tgctccagtc gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt
    11641 tttttgaaac aggtgcgaga ggtaatttgg cgaaatatag aacgcctgtg cgacagattc
    11701 gcgggtaagt gcggaggcat agcgttcgtc gatataatca cgaatagctt cgaatagagc
    11761 ctggctgcgt gaggcggtct gtatttggct gccgagcaaa tcgcggcagt ggctaagcag
    11821 gctggcgacg ataagtctgg ccgtttgctg ctcctgcgac tgcatttgca tttcattgag
    11881 cgtttgtagc agaaaagaac caatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag
    11941 attttgatat tgtttgccat cccattgcac gacgctgaag ccgagttgct gtttgccaaa
    12001 tagcacgcta aaggtcgtgg cgggggcttg ccattgtgga aaattccagc caccagcagg
    12061 gacgtacagc acatcgccgg gaactaacgc tgctccagcg ccggtatcaa taaactcacc
    12121 ttccagcaca atttccagac gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc
    12181 gttgctgctg gcaaagtaaa tctggcgcag ggacagcggg ccgttgatca ggcgggagag
    12241 cagatagctg acgtcgtgat acatgttatt cccttaggta tgtagacggg aaaacagtaa
    12301 ctggcattat gaaaaaaggc cacctgaaaa gtgtggcctg atgggagtga gatgtaaaca
    12361 ttgcggcagt ttcagtcgac ttgtattgcc ggatgcggcg taaacgcctt atccggccta
    12421 ccgccgcgat ctgtaggcca gataagacgc gtcaagcgtc gcatccggca aataacatct
    12481 ggctgataat tactgattac ccacctgatc gccatacggc agagtgtcgt aatcgatcag
    12541 tgcgtttttc ttatacgggt taccaatcca gcgggtagtt tctttgaact gcggattcac
    12601 cactgaacgg gttggatcgt aaccgtcgta agataagccc gccaaatctg accaggtgtg
    12661 gatcagttcc gccaggctgt atttacgatc aacatcctgt gagaaatcac gcggatgagt
    12721 cgcttgccat ttttctgacg tccacagcag gaacggaatg gtgtacatat gacgcgtcgg
    12781 attgtcttca ttacgcccct gagttttgtg cggcggcgtg tcgtaaacct cttcaccgtg
    12841 gtcagagaaa taaaccagga aaccgttcgg atcggtcgct ttaaagtctt taatcaggct
    12901 ggcaaccacg tgatcgttgt acaggttagc attgtcataa tcattatatg actccagctc
    12961 ttctgcgttt aatcccggtg gaacatgctc tgtattaccg tcaaatttgc cctgatcttc
    13021 cggataacgg tacttgtact tgatatgcgt acccagcaga tgaacgatga tcagtttctt
    13081 cggcgcaggg tccttcagca cttcctggaa cggcttcagc acgttggtgt cgtattcacg
    13141 cgcactctgc gtacgttgct ggttcatgta gtactgcttg tcggtctggc gcgaaaatac
    13201 cgtcagcatc gtattgcggg cggtcattgt ctgctggttg gtgatccaga aggttttata
    13261 acccgcctgt ttcatcatgt tcatcagcga cggctgcgtc agatacagat ccgggttctt
    13321 ttcattggca aaggtcagcg cctgttgcag gatttcaatg gtgtacggac gagacgtaac
    13381 cacgttatta aacacggtca gattcggatc ggttttatgc agcgcatcca gctccggcgt
    13441 ggtttcacgc ggataaccgt acaggctcat gcgtccacgc tgggtcgatt cgccaattac
    13501 cagcactaag gtgcgcggtt cgttacccga ttcatctttg aaattagcca gcggcggcaa
    13561 tgcattattt tcattcagta gctttgttag cgagtttagt tgctgacgat actgataata
    13621 gccggtcagg aattgccacg gtgcggcagg ctccatacgc gatgccaggt tatccaacgt
    13681 tttctcaaac ggcttgtttt tgataaacgt attcatggcg atcggatgca gaatcaagcc
    13741 ataaagtagg gcaaaagaga caacataacg ccacggcttt ggaatataga ccgggcgcag
    13801 gcgtgtccac agcagaactg ccaccgccgt ataggccagc gcgataagca caattttcag
    13861 gctgaaatac tggcttaaat actcgctggc ttcgttggtg ttggtttcga acatcacaaa
    13921 cagaacgctc tgcgagaact cctgaccgta gatgacgtaa tagcacagcg ccgccagaga
    13981 ggccgcccat agcaccacgc cgatcactgc ggcaataatt ttaatccgct tcggaaagag
    14041 gaataccggg atcaaccaca gcgaactgaa taacagcgag tcgcgaatgc cgttagtgcc
    14101 actataacca ctgatgtaaa taatggcctg tagcagagta gagaaaaacc aaaagtagag
    14161 cagtgcccaa cccagggctt tccagctaaa aaggggttta gcctggactt ctgtggaatg
    14221 catagtaaga acctgtcttg aaaaaaatat cgccgaatgt aacgacaatt ccttaaggat
    14281 atctgaaggg atatccagat tttgaataaa atgcagacag aaatatattg aaaacgaggg
    14341 tgttagaaca gcagtatttc agaaaaccct cgcgcaaaag cacgagggtt tgcaggagag
    14401 gaagattagc cggtattacg catacctgcc gcaatcccgg caatagtgac cattaacgcc
    14461 tgctcgacgc gaggatccgg ttcatggcct tctttttctg cctggcggga gcggtgcagc
    14521 aactcggcct gcaatacgtt cagcgggtcg gtgtaaatat tccgtagctg aatagactct
    14581 gcaatccacg gcagatcggc catcaggtga gaatcgttgg caatcgccag caccactttg
    14641 atgtcttctt cttgcagatt gcgtaactct ttacctaacg gccacagtgc tttgtcgacc
    14701 aggcgttggt catagtattc tgccagccac aggtctgctt tggcgaagac catctccagc
    14761 atgccgagac gcgtcgagaa gaatggccaa tcgcggcaca tggcttccag ctcgctctgt
    14821 ttgccatctt cgaccacttt ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc
    14881 aggcggtttt gcgtccaggc aaaaatccac ggaatggcgc gcagcgactc gacgccgccg
    14941 gtcgggcgac gtttcgccgg acgtgaaccc aacggcagtt tacccaattc ttgttccggc
    15001 gtagcggagc ggaagtaagg cacaaaatct ttgttttcac gtacgtagcc acggtagaga
    15061 tcgcaggaga tgactgacag ttcatccata atgcgacgcc agctctcttt cggctcaggc
    15121 ggtggcagca ggttggcttc cagaatcgcc ccggtgtaca gcgacaggct gctgacggtg
    15181 atttctggca gaccatattt aaagcggatc atctcgccct gttcggtcac gcgcaggcca
    15241 cctttcaggc ttcctggcgg ttgtgacagc agcgccgcat gggcaggtgc gccgccgcga
    15301 ccaatggaac cgccgcgacc gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt
    15361 ttgattaatg catcctgtgc ctgatattgc gcccaggaag ctgccatcac gcccgcatct
    15421 tttgctgagt cggaatagcc aatcatcacc atctgtttgc cctgaatcag gccgcgatac
    15481 cagtcgatat tcagcagctg ggtcatgaca tcgttggcgt tgttcaggtc atcgagggtt
    15541 tcaaacagcg gagcaaccgg catcgcaaac ccgatacccg cttctttcag cagcaggtgg
    15601 acagccagca cgtcggacgg cgttttcgcc atcgagatca cgtaggcggc aatggagcct
    15661 tgcggcgctt cggcaatcac ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt
    15721 tgccagttgc gcggtagaag cggacgtttg gagttcagtt cgcggatcag gaacgcctgt
    15781 ttgtcggctt ctgaccagct ctcgtagtcg ccgataccga ggtagcgggt cagctcgccc
    15841 agcgcttcgg tatggcgggt gctctcctga cggatatcaa tacgcaccag cggtacgccg
    15901 aaacatttca cgcggcgcag ggtgtcgagc agatcgccat tggcgataat acccatgcca
    15961 cacgcctgaa gtgactggta gcaagcgtag agcggttccc acagttcttc gttttgtgtc
    16021 agcagacctt ctggttttgg cagttcttcg cctttcaggc gcgcttccag ccatgcctgt
    16081 gtcgccatca ggcgagaacg caggtttttc atcagatagc gatacggttc tgcggcaccc
    16141 tcttcgccaa ccagtgccag cagttcaggg gtggcttcaa ccatcgacag ttcagaaacc
    16201 agaacctgaa tatctttcag gaacaaatcg gtggctttcc agcggctgag tagcaggacg
    16261 tggcgggtga tatcggcagt gacgttcgga ttgccgtcgc ggtcaccgcc catccacgag
    16321 gtaaaacgta ccggaacaaa ttcgacgggc agtttgtagc cgaggttctc ttccagttgt
    16381 tcgttcagtt cgcgcaggta attcggtacg ccttgccaca ggctgttttc tactacggca
    16441 aagccccatt tggcttcatc taccgggctt gggcgcagtt tacggatttc atcggtatgc
    16501 catgactggg cgatcaactg gcgcaggcgg cgcatcagct ggttgtgttc gtagtcggcg
    16561 atatctttgt tatcgagctg ttttaaacag gcgttcactt ccaccatttt gtggatcagt
    16621 gtacgacggg taatttcggt tgggtgagcc gtgaggacca gttccagcga cagcgattcc
    16681 actgcttttt tgatggtgtc ttcgctcagt tccggctggt ttttcagttt acgcagggtg
    16741 cgggcgatca cttccgggtt gctggcagct tcgcctttcg gcgaaatgct gtggtattgc
    16801 tcagcggtgt tggccaggtt cagaaactga ctaaacgcac gcgcaacggg cagcagctcg
    16861 tcgttcgaca aattttgtaa ggtggtgagc aactcctggc ggttagcatc attgccagcg
    16921 cgtgaagatt tggacaactt acggatagtt tctacgcgtt caagaatgtg ttctcccaac
    16981 gcatccttga tggtttctcc cagcactttg ccgagcatac tgacattact acgcaatgcg
    17041 gaatattgtt cgttcatatt accccagaca ccccatctta tcgtttgata gccctgtatc
    17101 cttcacgtcg cattggcgcg aatatgctcg ggctttgctt ttcgtcgtct tttataaagc
    17161 cacgtaaaag cggtgacgtc aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag
    17221 gaatttacgc cattaaattc acgacgcttt aaataagcgt aacttatggg aatgtt
//
LOCUS       DAOFSV010000090        17184 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.090, whole genome shotgun sequence.
ACCESSION   DAOFSV010000090 DAOFSV010000000
VERSION     DAOFSV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.090"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(5..1285)
                     /gene="hemL"
                     /locus_tag="QBH27_003149"
     CDS             complement(5..1285)
                     /gene="hemL"
                     /locus_tag="QBH27_003149"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDQ7015430.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMSCDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            1510..2931
                     /gene="clcA"
                     /locus_tag="QBH27_003150"
     CDS             1510..2931
                     /gene="clcA"
                     /locus_tag="QBH27_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDQ7015431.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            2955..3020
                     /locus_tag="QBH27_003151"
     CDS             2955..3020
                     /locus_tag="QBH27_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015432.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            3013..3357
                     /gene="erpA"
                     /locus_tag="QBH27_003152"
     CDS             3013..3357
                     /gene="erpA"
                     /locus_tag="QBH27_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDQ7015433.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3404..4027)
                     /locus_tag="QBH27_003153"
     CDS             complement(3404..4027)
                     /locus_tag="QBH27_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDQ7015434.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(4065..4865)
                     /gene="btuF"
                     /locus_tag="QBH27_003154"
     CDS             complement(4065..4865)
                     /gene="btuF"
                     /locus_tag="QBH27_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDQ7015435.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(4858..5556)
                     /gene="mtnN"
                     /locus_tag="QBH27_003155"
     CDS             complement(4858..5556)
                     /gene="mtnN"
                     /locus_tag="QBH27_003155"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDQ7015436.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            5640..7157
                     /gene="dgt"
                     /locus_tag="QBH27_003156"
     CDS             5640..7157
                     /gene="dgt"
                     /locus_tag="QBH27_003156"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDQ7015437.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLETYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSFFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            7287..8711
                     /gene="degP"
                     /locus_tag="QBH27_003157"
     CDS             7287..8711
                     /gene="degP"
                     /locus_tag="QBH27_003157"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDQ7015438.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGPGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            8866..10023
                     /gene="cdaR"
                     /locus_tag="QBH27_003158"
     CDS             8866..10023
                     /gene="cdaR"
                     /locus_tag="QBH27_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDQ7015439.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(10077..10463)
                     /locus_tag="QBH27_003159"
     CDS             complement(10077..10463)
                     /locus_tag="QBH27_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDQ7015440.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(10773..11597)
                     /gene="dapD"
                     /locus_tag="QBH27_003160"
     CDS             complement(10773..11597)
                     /gene="dapD"
                     /locus_tag="QBH27_003160"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDQ7015441.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(11628..14300)
                     /gene="glnD"
                     /locus_tag="QBH27_003161"
     CDS             complement(11628..14300)
                     /gene="glnD"
                     /locus_tag="QBH27_003161"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDQ7015442.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(14362..15156)
                     /gene="map"
                     /locus_tag="QBH27_003162"
     CDS             complement(14362..15156)
                     /gene="map"
                     /locus_tag="QBH27_003162"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDQ7015443.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            15524..16249
                     /gene="rpsB"
                     /locus_tag="QBH27_003163"
     CDS             15524..16249
                     /gene="rpsB"
                     /locus_tag="QBH27_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDQ7015444.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            16384..>17184
                     /gene="tsf"
                     /locus_tag="QBH27_003164"
     CDS             16384..>17184
                     /gene="tsf"
                     /locus_tag="QBH27_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDQ7015445.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEK"
BASE COUNT         4161 a         4286 c         4542 g         4195 t
ORIGIN      
        1 atcatcacaa cttcgcaaac acccgacgtg cagcatcgat ggtgttattg atatcttcca
       61 tgctgtgcgc cacagacata aagcccgctt caaacgctga cggtgccaga taaacacctt
      121 cgtccagcat catatggaag aaacgtttaa agcgttccac gtcacaggac atcacatcct
      181 ggtagcatgt cacggactcg gcgtcggtaa agaaaatacc gaacatgccg ccaacgtggt
      241 ttaccaccag cggaattccg gcttcttctg ccgcttcccg cagaccttct gccagacgtg
      301 ttgtcagctc atccagcgtc tcgtgaacgc ccggctgtgc gacttcattc agacaggcga
      361 aaccagcagc catcgcaatc gggttaccgg aaagcgtacc cgcctgatag accggaccgg
      421 tcggggccag cgcatccatt acatcacgac gaccaccgaa tgcgcctacc ggcattccac
      481 cgccgatgat tttgccgagg caggtgagat ccggttccac accgtaataa tcctgtgcgc
      541 cagctagcgc tacgcggaaa ccggtcatca cttcatcgat gatcagcaac gcgccaaatt
      601 cgtcgcacag cgcgcgcaga cctggcagga actctggcag cggtggcacg cagttcatat
      661 tgcctgccac cggctcgacg ataatacagg caatctcttg cgggtattgc tcaaacgcgg
      721 cgcgtacaga ggccagatcg ttataagtac aggttaaggt atgtttggcg aaatctgccg
      781 gaacacccgg cgaatttggc tggcctaacg tgagtgcgcc agaaccggct ttcaccagca
      841 agcagtcagc gtgaccgtgg taacaacctt caaatttaat aattttgtcg cgaccggtaa
      901 aaccacgggc caggcggatg gcgctcatcg tcgcctcggt gccggagttc accatgcgca
      961 ccatatccat ggtcgggacc agttcggtca ccagttgcgc cattttcact tccatttcgg
     1021 ttggtgcacc aaagcttaaa ccacgctcgg cggcttcaat cacggcattg cggatcgccg
     1081 gatggttatg gcccagcacc atcggccccc aggaaccgac ataatcgata taggctttgc
     1141 catcaacatc gtacagataa gcgccgtccg ctttttcgat aaacagtgga gtgccgccca
     1201 cgccagtaaa ggcgcgaaca ggggagttca cgccgccagg gatcagctcg cgcgctgcgc
     1261 tgtaaagatt ttcagactta ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct
     1321 gctattctat gttattcata acaagttaaa tactcgtcaa acatcaggct gcttgtactg
     1381 gtgcaatcct gatttcttag agtataaaag ttttgtgcat ttgaaacatt acgctttgca
     1441 aaggattttc atggaacgtg cgagtaaaat gccgtcatct tatttgtatg accaataagt
     1501 gatcattgga tgaaaactga tactccctct ttagaaacac cgcaggccgc gcgcctgcga
     1561 cgcagacaac tgattcgcca acttcttgag cgcgataaaa ccccgttagc cattttgttt
     1621 atggcggcag tcgtcggcac gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc
     1681 gcctggttgc agaaccaacg tatgggggca ctggtacata ctgctgataa ttatccgctt
     1741 ctgttaaccg tcgcttttct ctgttcggcg gtgctggcga tgttcggcta ctttttggtg
     1801 cgcaaatacg cgccggaagc aggtggttcg gggatcccgg aaattgaagg ggcgctggaa
     1861 gatcaacgtc ctgttcgctg gtggcgtgtg ttgccggtga aattctttgg tgggctgggg
     1921 acactcggcg gaggcatggt gttggggcgc gaagggccaa ccgtgcagat aggcggcaat
     1981 atcgggcgga tggtactcga tgttttccgc ctgaaaggtg acgaagctcg tcatacgcta
     2041 ctggcaaccg gtgctgctgc ggggctggct gcggccttta acgcgccgct ggcgggtatt
     2101 ttgtttatta tcgaagagat gcgtccgcag tttcgctata cgttaatttc gattaaagcg
     2161 gtatttattg gcgtcattat gtcgaccatt atgtaccgga tttttaatca tgaagttgcg
     2221 ttgattgacg tcggtaaact ttctgatgcg ccgcttaata cgctgtggct ttatctgatc
     2281 ctcggtatta tttttggcat tttcggccct atttttaata aatgggtgct ggggatgcag
     2341 gatttgctgc accgtgtgca cggtggcaat attaccaaat gggtactgat gggcggtgcg
     2401 attggcggtt tgtgtggatt gctggggttt gtggcaccag caacttcggg cggcggtttt
     2461 aacctgattc ctatcgctac cgcggggaat ttcagcatgg gaatgctggt gtttatcttc
     2521 gtcgcgcggg tcattaccac cttactctgc ttctcttccg gcgcgccggg cggtattttt
     2581 gccccgatgc tggcgctggg tactgtgctg ggaaccgctt tcggaatggt tgccgttgag
     2641 ttgtttccgc aatatcacct tgaggcgggg acgtttgcta ttgccggaat gggggcgtta
     2701 ctggcggcat ctattcgcgc gccgttaacg gggatcattc tggttctgga aatgaccgat
     2761 aactaccagc tcattttgcc aatgattatt accggtcttg gcgcaacact attagcacaa
     2821 tttaccggtg ggaaaccgct atactcggcg attcttgcgc gcacgctggc aaaacaggaa
     2881 gctgagcaac tggcgcgaag caaggccgca tcagccagcg agaatacttg aacgaaatac
     2941 cagggtatta gataatggcg attattattg ggttagaatt tgcccaattg ccgatgtcgt
     3001 ttggagcaaa atatgagtga tgacgtagca ctgccgctgg agtttaccga cgcagcagcc
     3061 aacaaagtta aaagcctgat cgctgacgaa gataacccga atctgaaatt acgcgtgtat
     3121 atcaccggtg gcggttgcag cggcttccag tatggtttca cctttgatga tcaggtgaac
     3181 gaaggcgata tgaccatcga aaaacagggc gttggcctgg tggttgatcc gatgagcctg
     3241 caatatctgg tcggcggttc cgttgactat accgaaggtc tggaaggttc tcgtttcatc
     3301 gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt cttcctttag tatctaatct
     3361 gctgttcatg tgccggataa actatccggc acatctcctc tcttcaacga ccattctcat
     3421 ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc cgccagccga atcaatagcg
     3481 tcacgaccat gcccatcata ctggctgttt ccagtggtac ggaaaatgtg taataagccg
     3541 tagcgtggac aataccgccg ataatacagg cagttgcgta gatttctgta cgtaaaatca
     3601 tggggatttc gcgggccagt acatcacgaa tgattccgcc gccaacgcca gtaatgacgc
     3661 ccatacaaac cgcgattaac ggaccggctt ccgcattaaa ggctttattc acgccaatgc
     3721 cgacaaacac cgccagacca acggcgtcca gcaccggcaa catccatttt ggtaagcgtc
     3781 ttggctggcg caccagcacg atggtcagca tgctggtgac cattgcaacg accagatcgg
     3841 tgggatcttt cacccaaaat accgggccgt gatccagcgc catgtcgcga attgttccgc
     3901 cgcctactgc ggtaaccacg cccaatacca gaacaccaaa agggtccata cgcaattttc
     3961 cggctaacaa aacgccagag atagcaaata ccgctgtgcc gactatatcc agccaataga
     4021 cgagcattgt tcaatcccca ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa
     4081 agcgcattac agagctgttg tgcagcgagg ataatacgtg ggcttgcacg ttcaaaccag
     4141 tcactcgtga gaggaataac gggaattttg agctgttcac cccagtattg tttgatttta
     4201 ggaatttggt ccggtccgcc tgtaatgaca atcgcctgtg gcgagcgtgc taacacctgt
     4261 tcgcggctaa cttgcggcca gggaacccgg ctgtctttaa agatgttttc tccgccacaa
     4321 acttcgagta cctggttctg aatcgactct tttccactgg taaatggcgg attaatgccg
     4381 aattgcagaa aaacacgttt tttaggttta tcagcatatt gcgctttcaa ttgcgcgtac
     4441 tgatccagca gggattgcgc ggcttgttcg gccttatccg gttgcggact ccagggggcc
     4501 agttgacgta acgcattggc aatttgttca atgcttgtcg catcgaccca catcactttt
     4561 attcccagcg aagccagctg gtcaacctgc cgctcggcat tacctccacg ccaggcaatc
     4621 accagatcgg gtttcagcgc gacaatgcgt tccagattca tcccctgcca ggtggaaacc
     4681 tgctcaatct tttgcgcttg tggaggatag tcggaatagc tgctgacccc aaccggcgtg
     4741 atcccggcgg caaaggcaag ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg
     4801 gcattgagcc acagcggcgc gagaaaagac agggcgacca gcgccctgaa cagtgactta
     4861 gccatgtgcc agtttctgca ccagtgactc aaccatcagg ctggactgtt tagcggcaac
     4921 agccaggaac tcatcgaagc taagatgaga ctgttgatcg gccacgtcgg agatggcgcg
     4981 tactacgaca aacgggacgt tgaaattgtg gcagacatgg gcgattgccg tcgcttccat
     5041 ctctacagca atggcctgtg ggaagttgtg gcggattttc gccagaccaa cagaaccgtt
     5101 gatgaaagcg tcgccgctaa caatcaggcc acgtacagcg ttaagattca gttcggcaat
     5161 gcaggcctca gcggcagcga tcagtttatc gtcagctttg aagcctgccg ggcagcctgg
     5221 taactgaccg tattcataac caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc
     5281 cgagacaacg atatcgccca ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt
     5341 aataatcaca tctggcttgc agtgttccaa cagcaaagtg gcacccagcg ccgcagcgac
     5401 tttaccgatg cccgatttca gaagcgcaac ctcggttcca ttcagttggc cggtatagat
     5461 ttcgcaaccg cccagactga tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac
     5521 ttcttcttcc attgcaccaa tgatgccgat tttcatagat ttactcgcga taagcccgat
     5581 ttgaaggcat agtttaccat gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca
     5641 tggcacagat tgatttccga aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg
     5701 gcgttaaaac cgaacatgag attctgcgga tcttcgagag cgatcgcggg cgtatcatca
     5761 actctccggc aattcgtcgt ctgcaacaaa agacccaggt ttttccactg gagcgcaatg
     5821 ccgccgtgcg cacgcgtctt acccactcga tggaagtcca gcaggtgggg cgttatatcg
     5881 ctaaagagat tttaagccgc ctgaaagagc ttaaattact ggagacgtat ggtctcgatg
     5941 aactgaccgg gccctttgaa agcattgttg agatgtcatg cctgatgcac gatatcggca
     6001 atccgccgtt tggtcatttt ggcgaagcgg cgataaatga ctggtttcgc cagcgtttgt
     6061 acccggaaga tgccgaaagc cagcctctga ctgacgatcg ctgcagcgtg gcggcactac
     6121 gtttacggga cggggaagaa ccgcttaacg cgctgcggcg caagattcgt caggacttat
     6181 gtcattttga aggtaacgca cagggtattc gtttggtgca tacattgatg cggatgaatc
     6241 tcacctgggc gcaggttggc ggtattttaa aatatacccg tccggcgtgg tggcgtggtg
     6301 aaacgcctga gacacatcac tatttaatga aaaagccggg ctattatctt tccgaagaag
     6361 cctatattgc ccggttgcgt aaagaactta atttggcgct ttacagtcgt tttccgttaa
     6421 cgtggattat ggaagctgcc gacgacatct cctattgtgt ggcagacctt gaagatgcgg
     6481 tagagaaaag gatatttacc gttgagcagc tttatcatca tttgcacgag gcgtggggcc
     6541 agcatgagaa aggttcgttc ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct
     6601 caaatagttt aagccgcagt acggaagatc agttttttat gtatttacgg gtaaacaccc
     6661 taaataaact ggtaccctac gcggcacaac gatttattga taatctgcct gcgattttcg
     6721 ccggaacgtt taatcatgca ttattggaag atgccagcga atgcagcgat cttcttaagc
     6781 tatataaaaa tgtcgctgta aaacatgtgt ttagccatcc agatgtcgag cagcttgaat
     6841 tgcagggcta tcgggtcatt agcggattat tagagattta tcgtccttta ttaaacctgc
     6901 cgttatcaga ctttactgaa ctggtagaaa aagaacgggt gaaacgtttc cctattgaaa
     6961 cgcgcttatt ccacaaactc tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta
     7021 aattaccgtc agattctcct gagtttccgc tatgggaata ttattaccgt tgccgcctgc
     7081 tgcaggatta tatcagcggt atgaccgacc tctatgcgtg ggatgaatac cgacgtctga
     7141 tggccgtaga acaataacca ggcttttgta aagacgaaca ataaattttt accttttgca
     7201 gaaactttag ttcggaactt cgcgctataa aacgaatctg aagaacacag caattttgcg
     7261 ttatctgtta atcgagactg aaatacatga aaaaaaccac attagcactg agtgcactgg
     7321 ctctgagttt aggtttggcg ttatctccgc tctctgcaac ggcggctgag acttcttcag
     7381 caacgacagc ccagcagatg ccaagccttg caccgatgct cgaaaaggtg atgccttcag
     7441 tggtcagcat taacgtagaa ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt
     7501 tccagcagtt cttcggtgat gattctccgt tctgccagga gggttctccg ttccagagct
     7561 ctccgttctg ccaggggggc ccgggcggta atggcggcgg ccagcaacag aaattcatgg
     7621 cgctgggttc cggcgtcatc attgatgccg ataaaggcta tgtcgtcacc aacaaccacg
     7681 ttgttgataa cgcgacggtg attaaagtcc agcttagcga tggtcgtaag ttcgacgcga
     7741 agatggttgg caaagatccg cgctctgata tcgcgttgat ccagatccag aacccgaaaa
     7801 acctgaccgc aattaagatg gcggattctg atgcgctgcg cgtgggtgat tacaccgtag
     7861 caattggtaa cccgttcggc ctgggcgaga cagtaacttc cgggattgtc tctgcgctgg
     7921 gacgtagcgg cctgaacgct gaaaactacg aaaacttcat ccagaccgat gcagcgatca
     7981 accggggtaa ctcaggtggt gcgttggtta acctgaacgg cgaactgatt ggtatcaaca
     8041 ccgcgatcct cgcaccggac ggcggaaaca tcggtatcgg ttttgctatc ccgagcaaca
     8101 tggtgaaaaa cctgacctcg cagatggtgg aatacggcca ggtgaaacgt ggtgagttgg
     8161 gtattatggg cactgagctg aactccgatc tggcgaaagc gatgaaagtt gacgctcagc
     8221 gcggtgcttt cgtaagccag gttctgccga attcttccgc cgcgaaagcg ggcattaaag
     8281 cgggtgatgt gatcacctca ctgaacggta agccaatcag cagctttgcc gcactgcgtg
     8341 ctcaggtggg cactatgccg gtaggtagca aactgaccct gggcttactg cgcgacggga
     8401 agcaggttaa cgtgaacctg gaacttcagc agagcagcca gaatcaggtt gattccagca
     8461 ccatcttcaa cggcattgaa ggcgctgaga tgagtaacaa aggcaaagat cagggcgtgg
     8521 tggtgaacaa cgtgaaaacg gggactccgg ctgcgcagat cggcctgaag aaaggtgatg
     8581 tgattattgg cgcgaaccag caggcagtga aaaacatcgc tgaactgcgt aaagttctcg
     8641 acagcaaacc gtctgtgctg gcactgaaca ttcagcgcgg cgacagcact atctacctgt
     8701 taatgcagta atctccctca accccttcct gaaaacggga aggggttctc cttacaatct
     8761 gtgaacttca ccacaactcc atacatcttc atcatccttt gggcatttgc acaatgccgt
     8821 acgttacgta cttccttatg ctaagccgtg cataatggag gacttatggc tggttggcat
     8881 cttgatacca aaatggcgca ggatattgtg gcacgtacca tgcgcatcat cgataccaat
     8941 atcaacgtaa tggatgcccg cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt
     9001 gaattgcacg aaggagcatt gctggtactt tcacaaggac gagtcgtcga tatcgatgac
     9061 gcggtggcac gtcatctgca cggtgtgcgg caggggatta atctaccgtt acggctggaa
     9121 ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg taaatatggc
     9181 gaactggtct gcatgacggc tgaaatgatg ctggaacagt cgcggttgat gcacctgttg
     9241 gcgcaggata gccgtttgcg ggaagaactg gtgatgaacc tgattcaggc tgaggagaat
     9301 actcccgcac ttactgaatg ggcgcaacgg ctggggatcg atctcaatca accgcgagtg
     9361 gtggctattg ttgaggtcga cagcggtcag cttggtgtgg acagcgcaat ggcggagtta
     9421 cagcaactgc aaaatgcgct gactacgccc gagcgtaata atctagtggc gattgtctcg
     9481 ctaaccgaaa tggtggtgtt gaaaccggcg ttgaactctt ttgggcgctg ggacgcagaa
     9541 gatcatcgta agcgagttga acaactgatt acccgcatga aagagtatgg ccagctgcgt
     9601 tttcgcgttt cgctgggcaa ctattttacc ggtcctggca gtattgcccg atcctatcgt
     9661 acggcgaaaa cgacgatggt ggtgggtaaa cagcggatgc cagaaagtcg ctgctatttt
     9721 tatcaggatc tgatgttacc tgtgttactc gacagtttgc gtggcgactg gcaggccaac
     9781 gaactggcgc gaccgctggc gcggctgaaa gcgatggaca ataacggctt gctgcggcga
     9841 actctggcgg cgtggtttcg ccacaatgtg caaccgctgg caacgtcaaa ggcgttgttt
     9901 attcatcgta atactctgga atatcggctt aatcgtatat cggaactgac cgggcttgat
     9961 ttgggcaatt ttgatgacag gttgctgctg tatgtggcgt tacaactgga tgaagagcgg
    10021 taggtcgatt acaaaattac agccgggtga gactttaccc ggcccacaga atgacattat
    10081 ttacgcgtca gtttttccag atcggcttca atctcgctga tcttattggt tactactgac
    10141 tccaggtgac gtaagtcatc gaggatctta cgcttaagat caacttcgct gcggtcacgc
    10201 tggcagattt gatcaagctc atcaatgata taccgtagat tcgggctgat ttcctggact
    10261 tctttataac cctgccccac accatcagcg acaaccgttt tacgctgacg cggatattta
    10321 aacttcacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat tttcagaata
    10381 tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt ggtaataccc
    10441 agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa ttgtgcttat
    10501 tttagcattt ggcctggccc gcccggctcg actgtgatcg cagaaagcct ttttcttcca
    10561 gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtgatg atattccatg
    10621 ggatggatta gcacatttat cttcattttg cgaattacat gacagtccga aacaagatga
    10681 aaacgtattt aaaaaagggg gaaaatgagt aggacgaatt attctgagcc agaaagcaat
    10741 cgggcagcgt cgtgctgccc gtgtacatac ttttagtcga tggtacgcag cagttcgtta
    10801 ataccaactt taccgcgagt tttcgcgtca actttcttaa cgataactgc acagtagaga
    10861 ctgtatttgc catctttcga cggcagatta cctgaaacaa ccacagaccc tgccggaacg
    10921 cgaccgtagt ggatttcgcc ggtttcacgg tcataaatac gggtgctctg accaatgtat
    10981 acgcccatag agatgacgga accttcttcg acaatcaccc cttcaaccac ttcagagcgt
    11041 gcgccgatga agcagttatc ttcaataatg gtcgggttag cctgcagcgg ctccagcacg
    11101 ccgccgatac ccacgccacc ggaaaggtgg acgtttttac caatctgcgc acaggaaccg
    11161 acggtcgccc aggtatcaac catggtgcct tcatcaacat atgcgccgat gttgacgtaa
    11221 gacggcatca gcacggtgtt acgggcaatg aacgcgccct gacgcactgc cgctggtggc
    11281 acgacgcgga agccttcttt ctggaaacgt gcttcatcgt agttagcgaa tttcatcggc
    11341 actttgtcga agtagcggct ttctgcccct tcgatcacct gattatcatt aatacggaaa
    11401 gagagcagca ccgctttttt caaccactga tgcgtcaccc actgaccgtc aattttttcc
    11461 gctacacgca gtgcgccgga atccagcagg gcgatcacct gatttaccgc ttcgcgggta
    11521 acggtgtctg cattggctgg cgtgatctcg gcacggcgtt caaaagcggt ttcaataatg
    11581 ttctgtaact gctgcattgt taaactcttt tcatatcagt aaacacatca ccctttatcg
    11641 tttggattga gggcctctgt caaccgctga tgcacttcct gctgcaactc gttattaagc
    11701 gcacgccggt cagcggtggc aattatgaat aaatcttcta ctcgctcgcc aatggttgta
    11761 attcgggcgc catgaagcga aattcccaga tcggcaaaaa ttttcccgac tcgcgccagc
    11821 agtccaggtt ggtcgagggc gatcagttcg aggaacgatt tgcggtcggt atgggtcggc
    11881 aaaaacgtca cttcggtttc aacagtaaaa tggcgcaatt tagcgggttg gcgacggggc
    11941 tgcggtggct gccagctact ttgtgtcagc acctgctcca gaccaaaccg aataacctca
    12001 tgacgatctg cggatagcgg gctaccatcg ggttccagca ctataaaggt atccatcgcc
    12061 ataccgtcgc gagtggtgaa aatttgtgcg tcgtgaacac ttaaattgcg gcggtctaat
    12121 tcggcacaga cggcggcaaa cagatagggg cggtccgggc tccagataaa aatctcggtg
    12181 cctccacgcg tagcctgcgg gctaagcagt accagcggtt tgcttaaatc atgctgtaat
    12241 aaatggcgtg cgtgccaggc cagttgattt gggctatggc ggacaaaata gttagcgcga
    12301 cagcgtgacc aaatttggtg cagcgcctct tcgtcgatgt tatccatgcg cagtagtgcc
    12361 agcgcctgga gttggtgatg gcgaacccgt tcgcgcatat ccggcgtgtt ttgcattccg
    12421 cgtcgtagct gcttttcggt ggcaaaatag agttcgcgca acagactttg cttccagcta
    12481 ttccacagtg tttcgttggt ggcgcaaatg tcagccactg tcaggcatac cagatagcgc
    12541 agacgatttt ccgtttggac ttcttcggca aactgcttga tgacttccgg gtcctgaata
    12601 tcgcggcgct gagctgtcac cgacatcagc aagtgctggc gaaccagcca ggcgaccagc
    12661 tgtgtttcgc gtgagttcag cccgtggagt tcggcaaaat gcactacatc ctgagcaccg
    12721 agaatggagt ggtcgccgcc gcgtcctttg gcgatatcgt gaaacagcgc ggcgatgaaa
    12781 atcagctcag ttgacggcag acgcggccag acgtccacac acaacggatg gcgctggcgc
    12841 gtttcttcac tggcaaaact ctccagtttc aacatcacgc ggatagtatg ttcatccacc
    12901 gtgtaggcgt ggaacagatc aaactgcatc tgcccgacga tatgcgacca ttgcggcata
    12961 tacgcgccga gtacgctatg gcgatgcatt ggcaatagcc cgcgccgcac cgctccgggg
    13021 tgacgcagaa tgctcaaaaa cagttttcgc gcttccggaa tattacacag aggttgttgc
    13081 agatggcgac gggcgtggcg taactggcgt agcgtggtgg agtaaatgcc ggtgatcgca
    13141 ctgttgcgca ccatggtgta gaacatacgc aagatggctt ccggctggcg cataaatagt
    13201 gtttcatcac gcaggtcgat cagcgttccg cgtagctgaa actcatcgtc gattggacgt
    13261 ggtttttcgt cggcgggaag ggcgaggatg gcttcatcga acagttgcag cagcatctgg
    13321 ttgagttcac tgacgcggcg tgtaacgcgg aagtaatcct tcatcatccg ctcgaccggt
    13381 tcgttacctt caccactgta attcaaacgc tgggcgacgc taagctggcg atcgaataac
    13441 aggcgattat cgtaacgact gacgaccaga tgcagggcaa agcgaatgcg ccacaatata
    13501 tgcaggcatt cgtttaattc cgctcgctcc gcagaggtta agaagccaaa ccctaccatt
    13561 tcatccagcg atgttgcgcc aaaatgacgg cgggccaccc attgcagagt gtggatatcg
    13621 cgcaagccgc cggggctact tttgatgtct ggttcaaggt tgtagctggt gccgtggtaa
    13681 cgctgatggc gctggttctg ttcttcaact ttcgccgcgt agaacttgtc ggaaggccag
    13741 aatccttcgc taaaaatatg tttttgcagt tcgaggaaca gcgcaacatc gccaattaat
    13801 aagcgggatt cgattaaatt ggtggcgacg gttaaatccg ataacccttc cagcatgcac
    13861 tcttcaagcg tgcgcacgct atgacccact tccagcttta catcccagag cagcgttaac
    13921 agctcgccca ctttttgcgc ctgatcgtcc gggagctttt tacggcttaa aatcagtaaa
    13981 tcgatgtcag aaagtggatg cagctcgcca cgaccgtagc caccgacggc gaccaatgcc
    14041 aggtcggcaa tctggctgaa tcccgcttca atccataatc gttgaagaag ctggtcgata
    14101 aactcggtac gcgcctcaat caactgttct gcggagatac cattgtcaaa ggcatcaccc
    14161 agccaacgct ggaaagtatc gatatgggct tttatcccac cgacggttaa ttcatcacgg
    14221 ggccaggcgc atggattttg cggttgaccg tgcagggtgg ggagagcggt gtttgcgtac
    14281 tgttctggaa gggtattcat tgtgcgccac ccataagatt aaattattgc attaaaaaag
    14341 ccggcattcg ccggcttcat tttattcgtc gtgcgagatt atcgccggga tggtgtcatc
    14401 cttgcgtagc gtcagaattt cgcagccgtt atcagtcacc acaatagtat gctcatattg
    14461 tgcagacaag ctgcgatctt tggtttttac cgtccagcca tctttcatgg tgcggatctc
    14521 ttttttaccc gcgttgacca ttggctcgat ggtgaatgtc atccccggtt tcagcacgac
    14581 gttggtttca cgggagtcat agtgcagcac ctgcggttct tcgtggaagc cgcgaccaat
    14641 accgtgtccg caatattcac gaacgacgga gaagccttct gcttcgacaa atttctgaat
    14701 tgccgcaccg atttcgcgca gattaatgcc tggttttacc atgcgtagcg ccaggtacag
    14761 gctttcttgc gtgatgcggc acagacgttc gcccatgatg gtcggcttac cgacgataaa
    14821 cattttcgag gtatcgccgt ggaaaccatc tttgattacg gtgacatcaa tgttaacgat
    14881 atcgccatct ttcagcagct tggcgtcgtc cgggataccg tggcacacca cttcattaat
    14941 agagatgcaa acggatttcg gatagccgtg atagccgagg caggcagaaa ccgcgtgttg
    15001 ttcattaaca atgtaatcat tacagatgcg atccagctcg ccggtgctga cgcccggttt
    15061 aacatacggt tcgatcatct ccagcacttc ggcagccagt cggccagcga cgcgcatttt
    15121 ttcgatatct tctggggtct tgattgagat agccattaat tctgtccatc agcgtcggtg
    15181 ataccgacaa tatatatgta agtgccgtca atggtatcac acccgggcaa attgagaatc
    15241 attctgaatt tcgccaaacg tgccactgaa ggttttctat aatagaaaat tcaacgtctg
    15301 atgctgtaca cagcgccaac aattattggt gtccacgacg tatttgtggt ataaagcgcg
    15361 ccggacttcc gatccatttc gtatacacag actggacgga agcgacaatc tcactttgtg
    15421 taacaacaca cacgtatcgg cacatattcc ggggtgccct ttggggtcgg taatatggga
    15481 tacgtggagg cataacccca acttttatat agaggtttta atcatggcaa ctgtttccat
    15541 gcgcgacatg ctcaaggctg gtgttcactt cggtcaccag acccgttact ggaacccgaa
    15601 aatgaagccg ttcatcttcg gtgcgcgtaa caaagttcac atcatcaacc ttgagaaaac
    15661 tgtaccgatg ttcaacgaag ctctggctga actgaacaag attgcttctc gcaaaggtaa
    15721 aatccttttc gttggtacta aacgcgctgc aagcgaagcg gtgaaagacg ctgctctgag
    15781 ctgcgaccag ttcttcgtga accatcgctg gctgggcggt atgctgacta actggaaaac
    15841 cgttcgtcag tccatcaaac gtctgaaaga cctggaaact cagtctcagg acggtacttt
    15901 cgacaagctg accaagaaag aagcgctgat gcgcactcgt gagctggaga aactggaaaa
    15961 cagcctgggc ggtatcaaag acatgggcgg tctgccggac gctctgtttg taatcgatgc
    16021 tgaccacgaa cacattgcta tcaaagaagc aaacaacctg ggtattccag tatttgctat
    16081 cgttgatacc aactctgatc cggacggtgt tgacttcgtt atcccgggta acgacgacgc
    16141 aatccgtgct gtgaccctgt acctgggcgc tgttgctgca accgtacgtg aaggccgttc
    16201 tcaggatctg gcttcccagg cggaagaaag cttcgtagaa gctgagtaat aaggcacgct
    16261 cgtacgagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc
    16321 ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt
    16381 agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc
    16441 atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa
    16501 aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac
    16561 ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag
    16621 actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca
    16681 gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt
    16741 gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa
    16801 ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gtgttctggt tgctgctaaa
    16861 ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa
    16921 ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg
    16981 gatatcgcga tgcagtctgg taagccgaaa gaaattgcag agaaaatggt tgaaggccgc
    17041 atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc
    17101 aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt tatccgcttc
    17161 gaagtgggtg aaggcatcga gaaa
//
LOCUS       DAOFSV010000091        17167 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.091, whole genome shotgun sequence.
ACCESSION   DAOFSV010000091 DAOFSV010000000
VERSION     DAOFSV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.091"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(25..363)
                     /gene="glnB"
                     /locus_tag="QBH27_003165"
     CDS             complement(25..363)
                     /gene="glnB"
                     /locus_tag="QBH27_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDQ7015446.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(424..1758)
                     /gene="glrR"
                     /locus_tag="QBH27_003166"
     CDS             complement(424..1758)
                     /gene="glrR"
                     /locus_tag="QBH27_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDQ7015447.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRNELDANDFKE"
     gene            complement(1748..2461)
                     /gene="qseG"
                     /locus_tag="QBH27_003167"
     CDS             complement(1748..2461)
                     /gene="qseG"
                     /locus_tag="QBH27_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDQ7015448.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(2626..4053)
                     /gene="qseE"
                     /locus_tag="QBH27_003168"
     CDS             complement(2626..4053)
                     /gene="qseE"
                     /locus_tag="QBH27_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDQ7015449.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETL
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(4629..8516)
                     /gene="purL"
                     /locus_tag="QBH27_003169"
                     /gene_synonym="purI"
     CDS             complement(4629..8516)
                     /gene="purL"
                     /locus_tag="QBH27_003169"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDQ7015450.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGMAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKDEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8774..10330
                     /gene="mltF"
                     /locus_tag="QBH27_003170"
     CDS             8774..10330
                     /gene="mltF"
                     /locus_tag="QBH27_003170"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDQ7015451.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(10327..10863)
                     /gene="tadA"
                     /locus_tag="QBH27_003171"
     CDS             complement(10327..10863)
                     /gene="tadA"
                     /locus_tag="QBH27_003171"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDQ7015452.1"
                     /translation="MRRAFITGVFFLSEVEFSYEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(10888..11523)
                     /gene="yfhb"
                     /locus_tag="QBH27_003172"
     CDS             complement(10888..11523)
                     /gene="yfhb"
                     /locus_tag="QBH27_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDQ7015453.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            11732..12580
                     /locus_tag="QBH27_003173"
     CDS             11732..12580
                     /locus_tag="QBH27_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDQ7015454.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            12636..12896
                     /gene="yfhL"
                     /locus_tag="QBH27_003174"
     CDS             12636..12896
                     /gene="yfhL"
                     /locus_tag="QBH27_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDQ7015455.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(13090..13170)
                     /gene="shoB"
                     /locus_tag="QBH27_003175"
     CDS             complement(13090..13170)
                     /gene="shoB"
                     /locus_tag="QBH27_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDQ7015456.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(13591..13971)
                     /locus_tag="QBH27_003176"
     CDS             complement(13591..13971)
                     /locus_tag="QBH27_003176"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDQ7015457.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(13971..14702)
                     /gene="pdxJ"
                     /locus_tag="QBH27_003177"
     CDS             complement(13971..14702)
                     /gene="pdxJ"
                     /locus_tag="QBH27_003177"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDQ7015458.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(14714..15442)
                     /gene="recO"
                     /locus_tag="QBH27_003178"
     CDS             complement(14714..15442)
                     /gene="recO"
                     /locus_tag="QBH27_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDQ7015459.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(15454..16359)
                     /gene="era"
                     /locus_tag="QBH27_003179"
     CDS             complement(15454..16359)
                     /gene="era"
                     /locus_tag="QBH27_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDQ7015460.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(16356..17036)
                     /gene="rnc"
                     /locus_tag="QBH27_003180"
     CDS             complement(16356..17036)
                     /gene="rnc"
                     /locus_tag="QBH27_003180"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDQ7015461.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
BASE COUNT         4104 a         4672 c         4299 g         4092 t
ORIGIN      
        1 acatccggca acccttgacg cgttttaaat tgccgcgtcg tcctcttcac cggtacggat
       61 gcgaatgacc cgtgccacgt cgaagacgaa gattttacca tcaccgattt tgccggtttg
      121 cgccgtgcga ataatggtat cgacacaggt atcgacaatg tcgtccggta cgacaatctc
      181 aattttcact ttcggcagaa aatccaccat atactccgca ccacggtaca gctcggtatg
      241 gcctttctgg cggccaaaac ctttcacctc agtgaccgtc atgccggtaa taccgacttc
      301 agccagtgct tcgcggacat cgtccagctt gaagggttta ataatcgcat caatcttttt
      361 catgctattc cttgaaaagg tcgcctgtct tttgatctgc taaacgtaac acataacgcc
      421 aattcattcc ttgaaatcgt ttgcatccag ctcgtttcgg gaaagcagtt tataaaattc
      481 tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg ttgcctttgg tgatttgcag
      541 cagcttacgc aaatagttga gttcaaactg attacgtgcc tcaacaaagg ttggcagcgc
      601 cgtattttca ccctccagcg cctgctccac cagcgcatca ctaattaccg gagatgaggt
      661 cagcgccaca cactgttcaa tcacgttgac caactggcgc acgttgcccg gccagcttgc
      721 agtcatcagg cgtttcattg catcggtgga gaacgcgcgg acaaacggtt tatgtcgctc
      781 tgccgcctgg cgcaacaggt gatttgccag tagcggaatg tcttctgtgc gctccgccag
      841 tgccggaatt ttcaggctga caacattgag gcggtaataa agatcttcac ggaattcccc
      901 acgcgccatc gcttttggca ggtcgcggtg agtggcggaa ataatccgca cgtcaatatc
      961 aatatcgcgg ttactgccca gcgggcgcac tttacgctcc tgcaacacgc gcagcagttt
     1021 gacctgcaac ggtgcaggca tatcgccaat ctcatcaaga aatagcgtac cgccttccgc
     1081 cgcctggaat aaaccttcgc gattgctgac agcgccagta aacgcgccac gtgcatgacc
     1141 aaacagctcc gactccagca attgctcggg taatgcgcca cagttaatag caataaatgg
     1201 cttgctgttg cgcgggctgg cgttgtggat agcctgggcg aaaatctctt tcccggtgcc
     1261 gctctggccg ttaatcaaaa cgctaacgtc tgattgtgcc accagccgcg cctgttccag
     1321 caagcgcagc atcagcgggc tgcgggtgac aatcgcctcg cgccaacgtt cgtcggtggc
     1381 tggcgcggat tgttccagcg catcgtcaat tgcctgatat agcgcatctt tatcgacagg
     1441 cttggtgaga aaactgaaca cgccctgctg tgttgcagca acggcatcag gaatagaacc
     1501 atgcgcggta agaataatta ctggcattcc cggctgcact ttctggattt cagcaaacag
     1561 ctgcataccg tccatttcat ccatccgcag gtcgctgatg actaaatcta ctttttcgcg
     1621 attcagtacc cgcaatcctt cagcaccact ttccgccgtg accacactgt agccttcgct
     1681 ggtcagacgc aggccaagca gtttcagcaa tcccggatcg tcatcgacca gtaataaatg
     1741 cgcaggttta tggctcatca ggagtgacct catcggtgga tggcgcgggc ttttcactct
     1801 cgtgcggcgt atccggcgag aaatttccag ccggtttgcg ggtcgagagc tggcgttcaa
     1861 tatcggtcag gttttccagc ttgcgagtag tgagttccag ttgctgttgt agaacgtgat
     1921 gttgctggcg caaagtatcc agctcgctgt cgctggactg ctggagtttg ctgtagcgtt
     1981 ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc gcgccaaagt tgatacagtg
     2041 ggcgaacctg tgccgggatc tcggtactta gcgcatcaat gcgcgcgacc agctggcggc
     2101 gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag gtattttgcc
     2161 agttgccgtc gtcatattgg cgggcttgct gacgcgactg cgcaggcatt aaacgatcag
     2221 cacaatccat cgcccgcagc cagtaaagcg gattggtttc ggttgattta ccttgcagcg
     2281 cccagatgtc gctacattca gtagaaagat agtcagccag ttgataaacc ggaatttttt
     2341 cttctgctgg cgtatcaatg gctggcctat tgtgactctg cacgcaaccc agcaatgcca
     2401 gacatggcag ccctgccagc cacagccgtc ggggcaataa tcgttgaaaa atgtgtcgca
     2461 tattcaccag acttaaagcc tatcccagtg ggcgtaattg ttgcaggcag tttggacatg
     2521 gacagcgcgg agaaaccgga gcgtacatgt cgtacgtgaa gatttcgagc actgcccggg
     2581 gccgaactga caaataaaat agcctgatgg gatgggctgt tagcattatt tcgtgttttt
     2641 cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga ccagatacag
     2701 ttccccttgc atacggcgaa tacaatccct ggcaatgctt aatcccagac cgctgccctt
     2761 caccgcccct tttcgctggt ggcttccctg aaaaaagggt tcgaagatca tggcgcgttc
     2821 ctcttgcgga atgggcgtgc ctgtattgat gacatcaata taaacccgcg caccatgtaa
     2881 actgctgcga aggcaaatgt taccggattc agccccgtag tgcaccgcat tggagtaaag
     2941 attatccagt acgctcatca gcagcattgg ctccgccagg caagctgttg ctttgagatc
     3001 aacgtcggta tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa ccaatgtctc
     3061 caccagcggt gctaactcaa cattctccag ttccaccgca ctgtccgcct gtttacggtt
     3121 gtaatcaagc agttgttcga tcagtttttg caaattgcgg ctgctgctat caagaatgct
     3181 caccacctct ttttgctctg gcgtaagcgg cccgacaacc tgatcagcca gtaattcagt
     3241 gccctcgcgc atactcgcca gtggcgtttt taattcatga gataaatgtc ttaaaaattg
     3301 atggcgttgg gattccagcc atgacaggcg ctcacttaac caaagaatac gttgcccaac
     3361 cgagcgtaac tcgctcggtc cactgaacga gacgctattg cccagagaac gcccttcccc
     3421 cagccggttg atcatgcgct cgatattttt caccggaccg ataatcatcc tcgtgaaaag
     3481 cagtaccatc accagactca ccagaaatag caccagcgat tgccaaccaa aatattgccc
     3541 acgttcggcg atttcacgct gaagttgctg cccacgagag aacaccactg tgcgcgtggc
     3601 ctgcaccatt tcggtattgg cactggcaaa ggcttccaga cgcgcggcgg cagcagcatc
     3661 gggaccgctg ttgttacact gaagttgagc cagattgttc aaatcctgac gtaatgcctg
     3721 gtagagttta tcgtccggca gcacgcctgc gtgggcatcg agcatttcgc tgtaacgctt
     3781 gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc agcacgcagt actgacggta
     3841 gctgcgctcc atctccagtg ccgcgttggt catcgcttca ctgcgccggg catcaataag
     3901 cgtagtgcgg ttaaccagcg ccgcctgatc gctaagtgca ttcaggcttt gccaggcttg
     3961 ccatgccagc accaacaggg gcagcagaat cagcaaaaat gccagcatta ccagttgtcg
     4021 taatgagcgg ggaaaaacgg gccagcgttt caaggcgtta ctctcgtcag acgcgaatag
     4081 cctgatgcta accgagggga agttcagata caacaaagcc gggaattacc cggctttgtt
     4141 atggaataag gcggtgccta actcgacgtt tcgcccgatg gttgatatag ctaagctgat
     4201 atcagaagtt ggacggcagg caccttgttg tgcgtcattc gaattttatg tagcacgtcc
     4261 cgaaggggct gacataagtc ggtgaatgag ccactgctta ctattatgca gtaactgtgc
     4321 caataaagaa aatagtttgg taacatactg attatacgct gctttgaggg gtttatggct
     4381 tctccgctat ttgaatgtaa atcagccact gtgtcgctaa aaagagacaa cttaagataa
     4441 acttattaga taatattaaa atcaatgagt taagtgtcac caaaaagcga cacggcaaac
     4501 cacccattgt cgtgatttaa agacacaaaa aagctcccga agttgggagc tcataatagc
     4561 ggttggtgct taatgccgga tgcggcgtga atgcctcatc cgggctgcaa taccaatggg
     4621 ttgacgactt accccaactg cttacgtgca ttgcggaaaa tgcgcatcca tgggccatcc
     4681 tcgccccagt tttccggatg ccaggagttg ctgacagtac ggaaaactcg ttccgggtgc
     4741 ggcatcataa tggtgactcg accactttca gtcgtgaccg ccgtaatacc gttcggtgaa
     4801 ccgttcgggt tagccgggta ggtttcagtg actttgccga agttatcgac atagcgcagt
     4861 gccaccagcc ctttgctttc cagtgccgcc agatgcgccg catcacgcac ttccacgcgc
     4921 ccttcaccat gagacacagc aatcggcatc tgcgagccca ccatcccctg caacagcagc
     4981 gacgggcttt gggttacttc taccagactg aaacgcgctt caaagcgatc ggaggtgtta
     5041 cgcacaaaac gtggccacaa ctcgctaccc gggatcagtt cacgcagatt agacatcatc
     5101 tggcaaccgt tacatacccc cagcgccagc gtttgcggac gatggaagaa ggttgcaaac
     5161 tcatcgcgta cacggtcatt gaacaggata gacttcgccc aaccttcacc agcgcccagc
     5221 acatcaccgt aggagaaacc accacacgcg accagggcgt ggaaaccctc caggcccgtg
     5281 cgtccagcca gcagatcgct catatgcacg tcgatagcat cgaagcctgc acggtggaaa
     5341 gctgccgcca tttcaacatg cgagttcacg ccctgctcac gcagcacggc aactttcggg
     5401 cgcacgccag tggcaataaa cggtgctgcc acatcttcgt tgatatcaaa cgacagtttc
     5461 acattcaggc ccggatcggc gtcgtttgat ttcgcctggt gttcctgatc ggcacactcc
     5521 gggttgtcac gcaggcgctg catctgccag gtagtttctg cccaccagac acgcagcgtg
     5581 gtgcggcttt cgctaaatac agtctgcccg ttggcagtaa tcacaaaacg gtcaccggaa
     5641 accgcctgcc caacataatg gacgcaatca gcaagcccat gctgtgccag taccgcttca
     5701 acggcttcac gatcagcagc tcgaacctga atcaccgcac ccagttcttc gttaaacaac
     5761 gccgccaggc gatcgtcacc cagagaggcg atatccgcgt caatgccaca atgaccagca
     5821 aaggccattt ccgccagcgt taccagcagg ccgccatcag agcggtcgtg atacgccagc
     5881 agcttacgct gtgcaaccag cgcctgaatc gcgtcataga agcctttcag ttgggcaaca
     5941 tcgcgtacat ctgccggttt gtcgccaagc tgacgataaa cctgcgccag tgccgttgcg
     6001 cccagcgcgt tattgccttt gcccaaatca atcagcaaca gtgcgttatc ttcggtagaa
     6061 agctgcggcg taatggtgtg acgtacatct tccacgcggg caaatgcgga aatcaccagc
     6121 gacaacggcg acgtcatttc gcgctcttcg ttaccttcct gccagcgggt tttcatcgac
     6181 atagagtctt tacccaccgg tatcgtcagg cccagcgccg gacaaagttc ttcgcccaca
     6241 gctttaacgg cttcatacag gcccgcatct tcaccagggt ggcctgccgc cgccatccag
     6301 ttggcggaaa gtttaatgcg tttgatatcg ccaatttgtg ttgcggcgat gttggttagc
     6361 gcttcaccga ctgccagacg ggcagaagcg gcgaaatcca gcagcgcaac tggcgcacgt
     6421 tcgccaattg ccatcgcttc gccatagtag ctgtcgaggc tggcggtagt gaccgcgcag
     6481 ttagcgaccg gcacctgcca cggccccacc atctgatcgc gcgccaccat gccggttacg
     6541 ctgcggtcac cgatggtcac caggaaagtt ttctccgcca cggtcggcag atgcagcacg
     6601 cgtttcaccg cgtcagcgat ggtgatccct tcacgggcca gcgcgtcgcc tttcgctttc
     6661 agcgtttgca catcgcgggt catcttcggc gttttgccaa gcagtacgtc cagcggtaga
     6721 tcaatcggct gattatcaaa atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct
     6781 tcaccaatca ccgcgtacgg tgcgcgttca cgcttacaca gttcgtcaaa cagcggtaac
     6841 tgatcggcag caactgccag cacgtagcgt tcctgggatt cgttacacca gatttccagc
     6901 gggctcatgc ctggctcgtc gctcagaata tcgcgcagtt cgaatttacc gccgcgcccg
     6961 ccgtcgctca ccagttccgg catggcgtta gaaagaccgc ctgcgccaac gtcgtggata
     7021 aacaggattg ggttggcatc accaagctgc cagcaacggt cgatcacttc ctggcagcga
     7081 cgctccatct ccgggttgtc gcgctgtacg gaagcaaagt cgaggtcggc atcagactga
     7141 ccagatgcca tggaagacgc cgcgccgccg ccaagaccga tgttcattgc cgggccgccg
     7201 agaacgacca gcttcgcacc aacgttgatc tcgtcttttt gtacgtgatc ggcgcgaatg
     7261 ttgccgatcc cacccgccag catgatcggt ttgtgataac cgcgcagctc ttcgccattg
     7321 tggctgttca ctttttcttc ataagtacgg aagtagccgt tcagtgccgg gcgaccaaat
     7381 tcgttgttaa acgccgcgcc gcccagcggg ccttcggtca tgatgtccag cgcggtgaca
     7441 atgcgctcag gcttaccgaa atcttcttcc cacggctgtt caaagcccgg aatacgcaag
     7501 ttggatacgg agaaaccaac cagaccggct ttcggctttg cgccgcgccc ggtggcacct
     7561 tcatcgcgga tttcaccgcc agaaccggtc gccgcacccg gccacggaga aatcgccgtc
     7621 gggtggttgt gggtttcaac tttcatcaga atatgcgccg gttcctgatg gaaatcgtag
     7681 cggcccgttt cgtggtcagc aaagtagcga cccacttcag aaccttccat tacagcggcg
     7741 ttatctttat aagcagagag aacgtggtct ggcgtggttt cgaaggtgtt tttgatcatc
     7801 ttgaacagcg atttcggctg ctgttcaccg tcgataatcc agtcggcgtt aaaaattttg
     7861 tgacggcagt gctcggagtt cgcctgcgcg aacatataca gctcgatgtc gttcgggtta
     7921 cgacccagct ttgtgaacgc atcctgcaga tagtcaattt catcttccgc cagtgccagg
     7981 ccaagacgca ggttagcgtc gattagcgcc tgacggccct gccccagcaa atctacgctg
     8041 gtgaccggag tcggttgatg gtgtgcaaac aactgctctg catcatctaa agcaaaaaag
     8101 accgtttcca tcatgcggtc gtgcagttca gcggtcacct gctgccattg ttcattggtc
     8161 agcgtaccgg cttctatata gtaagccatg ccgcgctcaa ggcggtttac ctgttgtaga
     8221 ccgcaattat gggcgatatc ggtcgctttc gaagaccagg gagagatggt gccaggacgc
     8281 ggggtcacca gcaggagttt gccttgcggg gcgtggctgg cgagtgccgg gccatatttc
     8341 agcaggcgtt caagttgtgc gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa
     8401 tggacatact cggcgtaaat agtgtgaacc gggagcctgg cagcctgaaa acgtgccagc
     8461 agtttgttga ttcggaatgc cgacaatgca ggcgaaccac gcagaatttc catcataagt
     8521 ctctcgtctt ctaagctttc ggtgtaccca agggggggaa acgggcgtca ttataaagaa
     8581 tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg
     8641 cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa aatgccgctc
     8701 atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc gcacgacaca
     8761 gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact
     8821 gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat
     8881 cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgactta
     8941 taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac agtttgccga
     9001 ttacctcggt gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga
     9061 cctcgataat ggtaacgccg acctgctggc ggcaggactg gtctataaca gtgagcgggt
     9121 aaaaaattat cagcctggtc ctacctatta ttccgtgtca caacaactgg tttataaagt
     9181 gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca ccgttgcacc
     9241 gggtcacgtg gtggttaacg atctccagac cctgaaagac acaaaattcc cggaattaag
     9301 ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa
     9361 actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga
     9421 actcgccgta gcgctcgata tcaccgatga acaaccggtg acctggttta gcccgttaga
     9481 tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagacgg
     9541 tacactggca cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga
     9601 tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga
     9661 gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca
     9721 ctgggatgcg caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa
     9781 caccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggcgg
     9841 cgtgcgttat ttacaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgaacg
     9901 gatctggttt gccctcgccg cgtacaatat gggctatgcg catatgctgg atgcccgcgc
     9961 cctgacggca aaaaccaaag gtaatcctga cagttgggct gacgtaaaac agcgtctgcc
    10021 tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc gtgggcatga
    10081 agcctacgct tatgtcgaaa atattcgtaa ataccagatt agcctggtgg gttatctgca
    10141 ggagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt
    10201 atcgcctacg gagctgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc
    10261 atcaaactat ttgacccact ctccctctct gctgttttcc agaaaaggga gtgaagagaa
    10321 acaaaattaa tctgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat
    10381 gcgaaagaag tcactgagca acgccgcaca ctcatccgcc agtattcctt ccgtaatttc
    10441 cacccggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg cagcgccagt
    10501 tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca ttgctccggc
    10561 acacattaca cagggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg
    10621 catcaccaga ccgccctgcc gtagggccat gatttctgca tgtgcggtgg gatcatgtcg
    10681 accaatcgga cggttccagc cttcgccaat aacacgattg ttatgcacta ataccgcgcc
    10741 gaccggtact tcccgctcat cccaggcacg cttcgccagc gtcattgcat gacgcatcca
    10801 gtattcgtag ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg
    10861 cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt
    10921 tacccgccag cgatgctggc aaaagtaaag cagcgggttg tcctggttac tgtcgctata
    10981 gccactgtac agccgcagcg gcgtgccgat tttgcgctcc agttgcgcga ccttctcatg
    11041 cccgaggcaa cgcatcgtca atacccaacc accatagcca cgctgaatct gactggcgat
    11101 aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg
    11161 agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc
    11221 ctgaaccagc ggaaaggcgg taacattgtc acgaaaccag cgtacgaaat cggcctgcaa
    11281 cgcctgcaaa cgcgcttcat tgtgaccaaa agtgcatccc cacagaagca gactcatcgg
    11341 ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct atgatcggca acaacggcag
    11401 aacaagtaac gcattcagcg gttggcgacg cagtaaataa cgcaaaaaac tgccgaacat
    11461 atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc
    11521 caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac
    11581 agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa
    11641 ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg
    11701 ttgtcgccat tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg
    11761 ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga
    11821 tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag
    11881 cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcactaa agttggcgtt
    11941 gagtgaagcg ctggcaagcc agccggaatc tccctccgtg cccattcata accaaatccg
    12001 cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat
    12061 gtacgcaacg ttaaacgtta atagtgaaga gaaactgcat gaatgcgtgg caatgttgcg
    12121 ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt
    12181 tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata tgcatgcgct
    12241 actcgctacc gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg
    12301 tgtacggcgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct
    12361 ggcgattacc ggctttactc ccaatgccct gcaacagcgt gcttctcatt gcctgtatac
    12421 cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg cgcagggaat
    12481 gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg
    12541 tattcgtcat agcgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat
    12601 gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc actaaaaaat
    12661 gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttcg atgggtgaac
    12721 atatctacga gattaacagc gataagtgta ccgaatgcgt agggcactac gagacaccaa
    12781 cctgccagaa ggtgtgtccg atccccaata ctattgtgaa agatccgcaa catgtcgaga
    12841 cggaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa atttaatatt
    12901 ctgctctgga agtagagtgt taattatttt atagagaaat cttaacgcta ttatatttat
    12961 ttaaatgatg acattagcat aatcattcac taagttaatt tatatagtat ctgcccagac
    13021 acttatttat agttattaaa ggcgcgtccg attggttcac cggacgcacc ttaagtacgt
    13081 ttccttgtgt tataagaaca aaaggagcag ctgtaaaaca gcaatgatga ttttgatgac
    13141 ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc ttaaacaagg aatgcagccg
    13201 ttcagattag cccttacatc tccccaaaac tgaacgtgcg agttattgga atcgagtgct
    13261 gcactccaca ccagagcttt gacgacacca ctcgtttcaa tgggggaatt cagtggcatg
    13321 gtgtaaagca cagcaaaatt gccaataacg aaaccgattt taatgtactt atcaaaaagc
    13381 ggtcacgtta atcttttcat caatgtcgat ttcttcgtaa aggctcgcgt tttttatgtc
    13441 agagattgca agttgcttgt acaggtcaac gaaattgatc cataatcatt ttcgttggaa
    13501 tcgtgtgctg cacaaaatta aagttaaaaa gtaaaacccc cgttccttac cagttcgggg
    13561 gttttacttt ataaagagaa cggtattatt ttaactttca ataattaccg tggcacaagc
    13621 atagtggcgc tcatcggcaa gcgttacatg catatttaca acgccgagct tttccgccag
    13681 ttttaatgcc tcaccccata gccgtagccg tggtttgccg agctcatcat tgaatacttc
    13741 aaactgatta aacgccagac cattgcggat ccctgtgcca aatgcttttg ccgccgcttc
    13801 tttcacagca aaacgcttcg ccagaaaacg cactggctgg tggtgcgttt tccagatttc
    13861 ccattcgtta tcgcttaata cgcggcgtgc caggcgctca ccggatcggg cgatcaccgc
    13921 ttctatgcga gcgatctcta cgatatccgt acctaaacct aatattgcca ttagccacgc
    13981 gcttccagca tcagacgctt catttctgcc accgcatctt tcagtccggt catcactgca
    14041 cgaccaataa tggcatgacc gatattcagt tcatgcatct cagggatggc ggcaatggct
    14101 ttcacgttgt gataggtcag accgtgtccg gcgttaactt tcagaccgag actagtggca
    14161 aaggtcgcgg ctttggcgat acgcgccaac tcttgcgcct gttcggcgtc agttttggca
    14221 tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc cgcagctttg
    14281 atctgctctt catcggcgtc gataaacaga gaaacctgga tcccggcatc tgccagacgt
    14341 ttgcaggcat cgcgcatttt ttcacgctgc cctgcgacgt ccaggccgcc ttcggttgtt
    14401 acttcctgac gcttttccgg taccagacag caaaaatgtg gcttcgtctc aacggcgatc
    14461 gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag cgtctggcgc
    14521 aggatgcaca catcgcggtc ggtaatgtgg cggcggtctt cacgtaaatg cacggtaatg
    14581 ccgtccgctc ccgcctgctc agcaataaac gccgcctgca ccggatccgg gtaagcagta
    14641 ccgcgcgcgt tacgcagtgt tgcaatatgg tcaatgttga cgcctaacag taattcagcc
    14701 atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata aactgccgga
    14761 acagttccct gctctttaat ggtttaccgc caagatacgg cttaagcgcc atgcgggtaa
    14821 agcgtttcgc ggcgcgcagt gtgtctgcgt caggaaattc ccgcgcgttt aacgctttta
    14881 actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac cctttttctt
    14941 cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca caatgggtaa
    15001 aattgacgcc atagcccaga tgcccgagca gtgccagttc aaagcggcgc aacgcgggtt
    15061 ctggcgtacc agtgaccccg gcaagagact gaatgcagtg caagtaatcg aaaaagagtt
    15121 cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg tacagaccgc
    15181 tgtaaagcgt gataccgctt aatggcagcg ccagcgagac agcttcagca ctgcgcagcg
    15241 ttttgacttc gccacgcccg ccaaaacgta gcaagagagg ggtgaaaggc tgtaatgcac
    15301 ctttcagagt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg cgacccgatt
    15361 cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta tgcaggacaa
    15421 atgcgcgctg ccagccttcc atcggagtta ctcttaaaga tcgtcaacat aaccgagact
    15481 gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca gctcaaggtg
    15541 aacaggcgct tcgaacattt cctgcatgtc tttacgcgct tcaatcccga tggttttgat
    15601 cttggcccct ttgttgccaa tgaccatctt cttctgccct tcacgctcaa cgagaatcaa
    15661 accgttgatg tcataaccgc cgcgttcgtt agagacgaaa cgttcgatct ccacggtcac
    15721 ggagtacggc agttcagcgc cgagaaaacg catcagtttt tcgcggataa tttcagacgc
    15781 cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatggg tcgcttcagg
    15841 cagatgctta cgcacgattg ctgcaatagt gtcgacattc agcccggttt cggcagagat
    15901 cggcacgata tcgaggaagt tcatctggct tgccaggaac tgtaagtgtg gcagcagatc
    15961 ggctttttcc tgcacattat ccactttgtt caccgcgagg attaccggcg ctttgccatc
    16021 gcgcagtttg ttgagcacca tttcatcgtc cggcgtccag cgagtgcctt ctacgacaaa
    16081 aatcaccagc tcaacatcgc caatggagct gctcgccgct ttgttcatca gacggttaat
    16141 ggcgcgtttt tcttccatat gcaggcctgg ggtatcaacg tagatcgcct gatacgcgcc
    16201 ttcagtatgg atccccacaa tgcggtgacg agttgtttgc gccttgcggg aggtaatgga
    16261 gatcttctgc cccagcagtt tgttcaacaa tgtggatttg ccaacgttcg gacgtccgac
    16321 gatagcaata aatccgcagt aacttttatc gatgctcatt ccagctccag ttttttcaac
    16381 gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc aaccaccggt
    16441 tcactcaggc cgctgacctg gcagtggata gtaaattcct gatcgtgcgc ttcgccacgt
    16501 acctggacta ccagataagt tggcagcggc agatggcgac cctgcaaata ttcctgcaga
    16561 cgcgttttcg gatctttttg tttatcgcct gggctaattt cgtccagacg agtttgatac
    16621 cagttgagga ttaatttctc gacggtttga atatcactgt cgaggaatac gccaccaatt
    16681 aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct tttaagttca
    16741 cctggcccta aacgtaagca ctcacctaac tcaaattcgc gcgccagttc agccagcgta
    16801 ttaccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac acgagggaaa
    16861 cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcacctaa aaattccaga
    16921 cgctcgttat gtttactgct ggcactacga tgagttaatg cctgctgcaa cagttcctga
    16981 tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg gttcatgcga
    17041 taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata tacatggctc
    17101 ggctaactgc ttcactgcag tttcagttta ccggtatatg gggaccaacg ctgtttcgtg
    17161 tgccgtg
//
LOCUS       DAOFSV010000092        17086 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.092, whole genome shotgun sequence.
ACCESSION   DAOFSV010000092 DAOFSV010000000
VERSION     DAOFSV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.092"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(7..2250)
                     /gene="pdeF"
                     /locus_tag="QBH27_003181"
     CDS             complement(7..2250)
                     /gene="pdeF"
                     /locus_tag="QBH27_003181"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDQ7015462.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLHIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNVPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2432..2584)
                     /locus_tag="QBH27_003182"
     CDS             complement(2432..2584)
                     /locus_tag="QBH27_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015463.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKEASVNTYFNVMEVNLIIYND
                     DVIMMS"
     gene            2602..2793
                     /locus_tag="QBH27_003183"
     CDS             2602..2793
                     /locus_tag="QBH27_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDQ7015464.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            3095..3613
                     /locus_tag="QBH27_003184"
     CDS             3095..3613
                     /locus_tag="QBH27_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDQ7015465.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            3629..4168
                     /locus_tag="QBH27_003185"
     CDS             3629..4168
                     /locus_tag="QBH27_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDQ7015466.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(4261..5838)
                     /gene="guaA"
                     /locus_tag="QBH27_003186"
     CDS             complement(4261..5838)
                     /gene="guaA"
                     /locus_tag="QBH27_003186"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDQ7015467.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(5907..7373)
                     /gene="guaB"
                     /locus_tag="QBH27_003187"
     CDS             complement(5907..7373)
                     /gene="guaB"
                     /locus_tag="QBH27_003187"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDQ7015468.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            7535..8902
                     /gene="xseA"
                     /locus_tag="QBH27_003188"
     CDS             7535..8902
                     /gene="xseA"
                     /locus_tag="QBH27_003188"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDQ7015469.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFHPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGATLHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVHQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGDTLTTRLGDGVVVSEVSAVTKTRK
                     PRKKT"
     gene            complement(8956..17037)
                     /locus_tag="QBH27_003189"
     CDS             complement(8956..17037)
                     /locus_tag="QBH27_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HDQ7015470.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAEVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDA
                     QQTIPLLYLLTSTGYPYEGTVVLADQKVTLNIAPAQGDLISVSASGYNETLGAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLDSTELTTTVTEYR
                     GTSDANGTATITVTQPNGPGVKTPLVVGISGIAQTSEAAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQGDYYWSSLAGLTGQHHAADVSNRGETQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLAITDTKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDTAWEAHPVQIAGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITARMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QGYLSAVEQATHSSVDLGNGSVDSYSGFKPNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINSLNNIPVPFTAFTITKGMGYNRAGQVSGFDDPSSGAITMDNT
                     QYGTSQSSKVYAGITDARGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVTGAQMWGHMDETITVDSSTFTRPKLASEVASPDGTLTENNEIWARVSQANTS
                     STSKGGCDTNMLPRRSQLSALYNANSGNAVQTAHGWPTQRQPYWSNSPADKTPHFFTI
                     ALNDGAQTIGGDTPVYVSCLKTANKPASSITLEVVDKAQWNTGNSAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLNRGDGYTRSGEKHVAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTDNDGSKILNITRPDTHGTKTALTAMLYSDATKKSSIDTIFTVVTSPDSRL
                     AKMWGHMPETVTAADGTVFKRPLLLKELAYQTGRTSTSEDNENWALFNINYASFSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTTLELLTCQTAPIAAVSQILLEAADPVTLDTTYNVVKAKKSEESVVRV
                     ITKDAQGNLVGNTAFILTRANSVSRANSSATMSVGALTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTFTLKQDNSTGLRTDLTAKLDTSSSVKAMLPVVFTVVTSPDSPKANFWG
                     HMAETVTASDGSVYKRPLLFGELTNNYARYSTFENGESWARFTSTQSADTSMNGCGTD
                     YMPTLDGLKSLYDANRGNAMNTVQGWPVNMSYLTNTPSNTQTGSRYYNVVQLNSGAVS
                     QIVSTVLALQTCRTTPLMTASQITLEASDPGQFVSIDSTLSAVKAKKGDEVSIRISTK
                     DAQGNLVGNTPFALKHANSINRQNVTSSQKVSVTTAAGATVDTSATTILYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLSVIFTVTTSPDTPLATNWGHMAETFTS
                     SEGVTFKRPFLKAELSGGTAKTANNEVWSLLTATEKADVSKAGCDEAYQPLTTDMQGL
                     YVDYPNGQLATVLGLPTAAGNWWDYDMMAVAGGSWSNQAFVPGNGQLIQASSSYTAIV
                     MCLVEPHTEAVTIELTSTAQDAAKSLSNDGRPSAVAKKGELIPLTVTVRDSAGNPMPY
                     ADFTLSRETTLDRAKATVNTSADDLTVTALVPVSRDSVLTASGAKTLATTGSDGTATF
                     EVGQYSTTGLATPLTVTLARDTTKVATLDVIYTVITSPDSPSAKFWGHMPDTFTSSAG
                     VTFKRPLLKTETSSGLSISSNGEVWSYMSNLQNLTSTDCPLENQPRSKELLDLYSDHP
                     NGAIMTDLGLPMNAGNWWAYDMAILGTTTWSYQTVSLRTGAIAKNSATNALMLCLAQP
                     HPAGVSVTLTSSALDDARTLSNGGRPSASVKKDNPLPIVVMVKDRDGNPLAGEAITLR
                     RDNAMSRSGTIVNTAANVMVLTELAPVSATFTLASHGTQWLGFTGSDGTAIFNVEQPD
                     TVGLATPFTAILARDTETTTVLDAIFTVVTSPDSAMAAYWGHMPDTVTAENGVVFERP
                     KLWKELPSTSGVSKVNSNNEVWPVFNATQRADSNLSPCEAARRPLSDDLESLYARYPG
                     NTLTTQIGWSTQYTWWALDNWLGGSNPQVVGLNNGSAWGTVNTAYQGCLVNPRATVSS
                     VTLTSTALDSSTQAAKVKKGDAVPVVVTVKDSVGKPVPNISFILKRGDATPRNSGATL
                     YGDVDTMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVGQDNTPGYKTPLTVT
                     LTDNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKMLHRPLLKSELPSGVTA
                     AATPNVNNETWALAHVIDSTKWDVARQCGSMNNVPSSAELQTLHSGFSTLGWPSSISF
                     PYLSTDKAGSFYCGVEEGSGSLNCGIQPAKTPGFATCFQ"
BASE COUNT         4074 a         4605 c         4248 g         4159 t
ORIGIN      
        1 cattattcag gcactttcgc gaatgggttc gatttcattc agcgtatcaa ttaacggttg
       61 cggcttacca ataagataac cctgcatata atcgatcccc aaagagagca ccgcctcgcg
      121 gatctcttcg ttttcaacgt actctgccac tacccgcatt ttcttcattc gcgccaggtg
      181 gcaaatcgac gccactatct gataatccag actatttgac acaatattgc ggataaaact
      241 gccgtcaatt ttaagcagat cggcattcac atttttaagc cgcgcatagc tggcgtagcc
      301 ggtgccgaaa tcatcaatcg caatctggca gcctaattcc tgaagatgct gcaaggtgat
      361 ttgcgcctgc ttaacattgg tcagagcatt actttcggtg acttcaaaaa taagttgcca
      421 cgcttcaatc tgatatttag ccagcagctg actgacttca acaggaaaac gagcctgaca
      481 taccgaggtt ggagacagat taatagcaaa acggtgagcg ggcatcttcg ctctgttttc
      541 agccataaat tgcagcgtat gctcaatgac ccacatgtcg atactcgacg ataaaccaaa
      601 ttcgtgcgcg accggtaaga agctatcggg gctgatcagc tcatcattct cacccttcat
      661 gcgcagaaga atttcatggt aaacatcacc acgcataccg gtaatcggct gggccatcag
      721 gaaaaaatgg ttgtgttcca gcgcctgctg tagtcgattc atcatcgcga ctttatcttt
      781 caattcgcgt tgcaaataca ttgctccgcg acgctgcata ttttccggga cgttggtcac
      841 gatggaaagt tcggccaccg tatttagctc tcccagcagc aggtagatat gattcactgg
      901 cgagcgcaca tagcagtaac tgacgccaat ctgcggttgc atcggcattc catcccaaaa
      961 gaaacgaaat tgcttgagat ggctatccag tgcggtaatg cgctcctggt gcgattctgt
     1021 attcagccgc agcgcgagat cgttacccga aagctgataa acatcttcac ccggttccag
     1081 caatggtgac agccagtgag aaagtttttg cttgtattga atccgcagca tgatgccata
     1141 gttcttaacc agcatttcca tgccagggat gtgtaaatag catagcgcag accagggggc
     1201 atcacgcaac gcgcgattca gggcgcgaac atttggcaga tgaaccaccg ggtccacata
     1261 cgcaagccgc tgaatgcgtc tgactacagc tcgctgacgg gttgccagta cagccatata
     1321 attgacaata aaagagaata ccagataact ggaggaggtt atggtcagct gcgtggtata
     1381 gccaggataa atgggaatgt aattttgata gctatggatg ctgatcatca acacgaccgc
     1441 ccagagcaat gaaatcaact tataaccata gcgcatcgct ccccacatca tcaggggcag
     1501 caataatgac aaggtgtaat tggtgctaaa aatcgtgctt ttttcattta acggcatgca
     1561 taacagcaac aataaggcac ctaatgccag tagccagatt gcgaactctt ttttggtaac
     1621 tttggcatca acctgctgtt ttaattgcga ataatagcta cgtaaataaa acggatttcg
     1681 caccacccga atgataaagt agcacagcgg gacaccaatc agattaccca ccagcaaggc
     1741 ctgataattg attaaggtcc cgaggttaaa gggcatgacg ccgaccagat tttctctgct
     1801 cgccagtaac cctacaaacg cagcaaactg gaaaagtatc agaaacagcg ttgcaggaaa
     1861 cacaatctgc cagaatagtc gctgggaaat taaacggcta tcaccatgtg aaacgttgtt
     1921 acgccggggg gtaaaaaccc tgtaaccgcc ccaacagaga atgataatga taataaaatt
     1981 agccgttaat gagagcgttt cgtaaaaacc cagctctgca tatttgcgga caaaaatccc
     2041 taaggcgatc cctggcaatg ccgcccagct gaaaaacatc atcatgctga tcatcagtgc
     2101 cagtggcaga taaaagagaa aaacttcacc ggaagaaata tgcgcgaaag tattaatgtg
     2161 ggcgaaaatg ggcaagatta aagaaggcag gaacagcgga agcccccacc atttatcacg
     2221 tatttttata taagttgcat tcagtttcat agatgctcag cagaatcccc cacatcctga
     2281 aggaggtgta ttcagacagg catcccacct gacttcgaat gatgattatt catcactata
     2341 gagagcaatg attctaagtg tcatatgaaa gttccaattg atatatatca aacaaaataa
     2401 ccctgattaa tgaattatta cgtttatcat gttaactcat cattattaca tcatcattgt
     2461 aaataattaa attaacttcc ataacattaa aatatgtatt cactgacgct tctttacata
     2521 acgaagaatt gaccattttg tcctgttgtg ccttaatgta agtaccgtcc acggcgtggg
     2581 acatacttca aggaaccttt tgtgagtcag gcaaccagta tgcgaaaacg acaccgattt
     2641 aacagtcgca tgacccgtat cgtactgctc atcagcttta tcttcttctt tggccgtttt
     2701 atctactcgt ccgtcggtgc ctggcagcac catcagagca aaaaagaagc tcagcaatcc
     2761 acactctccg tcgaatcacc ggtacaacgt tagcggttac cttctccact ctcacagaac
     2821 ataacggcac ctcgctgtcg gatgcttttg ctctttgaga ttatcaaagc ggcagatatt
     2881 ctttcatctt aaattttacg tctttgttct gactgcagtt cggctgcgaa ataaatacat
     2941 cattaataca tatgttgtga tgatgtattt ttataaatta ttccccacat gaattttaat
     3001 aaatttaatc tattccttta tacgcaatac atttactttc ctcttttgat gatcttaaat
     3061 gtcttatttt tcgtaatgtg tataacaagg aatagtgatg aaatttaaaa aatgtcttct
     3121 gcctgtggca atgttagcgt cattcactct ggcaggatgc cagtcaaatg ctgatgatca
     3181 tgccgccgat gtttatcaaa ccgatcaact gaataccaaa caagaaacta aaaccgttaa
     3241 tattatttcc attcttcccg caaaagttgc cgtagacaac gcccaaaata aacggaacgc
     3301 acaagccttc ggcgcgctta ttggcgctgt cgctggcggt gttatcggcc acaacgtcgg
     3361 gtctggcagc aattccggaa cgacggcagg tgcagttggc ggcggagctg taggcgcggc
     3421 agcgggttct atggtgaatg ataaaacctt agtggaaggt gtttctttaa catataagga
     3481 aggcaccaaa gtgtatacct ccacccaagt gggtaaagag tgccagttta cgacaggttt
     3541 agccgttgtt attaccacga cgtataacga aacgcgtatt cagccaaata ccaaatgtcc
     3601 tgaaaagagc taataatcag gaggagtcat gaagaaagtt tttctttgcg ccatcttagc
     3661 ctccttaagc tatccggcta tcgcctcatc attgcaggat caactctcgg ctgtcgcaga
     3721 agcggaacag caaggtaaaa atgaagagca aaggcagcat gacgaatggg tcgcggagcg
     3781 caacagggaa atccagcaag agaagcaacg tcgcgcaaac gcccaggccg cggctaataa
     3841 aagagcggca acggcagcag cgaataagaa agctcgtcag gataaactgg acgccgaagc
     3901 cactgcggac aaaaaacgcg atcaaagtta tgaagatgag ctacgtagct tagagattca
     3961 gaaacaaaaa ctggcgctgg cgaaagaaga agcccgcgtt aagcgcgaaa acgaatttat
     4021 cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg gtgcaatctg aagctgacgc
     4081 aaacagaaat atgactgaag gcggtcgcga tctgatgaaa agcgtgggta aagcagaaga
     4141 gaataaatca gatagctggt ttaactaaac gatgttagta acttcaaacc tataattctt
     4201 taggataaaa aaccctctgt agtaacagag ggttttgttc attcatagtg cagggtcaaa
     4261 tcattcccac tcaatagttg ctggtggctt gccgctgatg tcatacacca cgcgggaaat
     4321 accattcact tcattgataa tgcggttgga aactcgaccg aggaaatcgt acggcagatg
     4381 tgcccagtgt gcggtcataa agtcgatggt ttcgacagca cgcagagaga caacccagtc
     4441 atacttacga ccatcgccca ttacgccaac ggaacgtact ggcaggaaga cggtgaacgc
     4501 ctggctgact ttgtcgtaca ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc
     4561 acggcgcagc aggtcacaat actctttctt cacttccccc agaacacgaa cgccaaggcc
     4621 tggtcccggg aacgggtgac ggtacaacat gtcatacggc aggcccagct ccagaccaat
     4681 cttacgcact tcgtctttga acagctcttt cagcggttca accaggccca tcttcatctc
     4741 tttcggcagc ccccccacgt tgtggtgaga tttgatgacg tgtgctttac cggttgcaga
     4801 agcagcagat tcgataacgt cagggtagat ggtgccctgc gccagccact tcacgtcttc
     4861 cagtttcagc gcttcttcat cgaatacttc aacgaaaacg cgcccgatga ttttacgttt
     4921 tgcttccgga tcgttttcgc cagccagcgc tgacaagaag cgatcttctg ccggtacgtg
     4981 aacaatgttc agaccaaagt gatcgccaaa catatccaga acctgctctg cttcgttgag
     5041 acgcagcagg ccgttgtcga cgaatacgca agtcaggttt ttaccgatag cgcggtgcaa
     5101 cagcattgcg gttacggagg aatccacgcc accagagagg ccgaggatga ctttatcgtc
     5161 gcctacctgc tcgcggatgc gggctacagc gtcgtcgata attttcgctg gcgtccacag
     5221 ggcttcacac tggcagatat cacgcacaaa acgctccagc atgcgcatac cctggcgggt
     5281 gtgagtcact tccgggtgga actgtacgcc atagaagcgt ttttcttcgt tggccatgat
     5341 ggcaaacggg cagctttcgg tgctggctac ggtgacgaag tcggacggga tagcggtaac
     5401 tttatcgccg tggctcatcc agacgtccag cagcggttta ccatctgcgg tcagagcatc
     5461 ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca acctgcgcgt agccaaattc
     5521 acgttcatta gaagcctcaa cgtgaccgcc caactgcatc gccatggtct gcataccata
     5581 gcaaacgccg aataccggta cgcctgcttc aaagacatac tgcggcgcac gcgggctgtt
     5641 ttcttcagta gtactttccg ggccgccgga aagaataatg ccgcttggat tgaagtcacg
     5701 aatttgtgct tctgtcacat cccacgccca cagttcgcag taaaccccca gttcacgcac
     5761 gcggcgcgca accagttgag tatactggga accgaagtcc agaatgagga tgcgatgctt
     5821 atgaatgttt tccgtcattg acgcttattc cgaggcaagt gaaacagata atataaatcg
     5881 cccgacatga agtcgggcga agagaatcag gagcccagac ggtagttcgg ggactcttta
     5941 gtaatggtca cgtcgtgaac gtggctttcc tgaatacccg caccgctgat acgtacaaac
     6001 tccgctttag tacgcagttc gtcgatagta ccacagccgg tcaggcccat acaggagcgc
     6061 aggccgccca tctgctggtg aatgatctct ttcaggcgac ctttataggc tacgcgacct
     6121 tcgatacctt ccggcaccag tttgtcggca gcgttgtcgc tctggaagta acggtcagaa
     6181 gaacctttgg acatcgcgcc cagggaaccc ataccacggt aagatttgta agaacggccc
     6241 tggtagagtt cgatttcacc cggagattct tcagtacctg ccagcatgga acctaccatc
     6301 accgcgcttg cgccagcggc gatagcttta gcgatatcgc ctgagaaacg gataccgccg
     6361 tcagcgataa ccggaatgcc ggtaccttcc agggcttcta ctgcatcagc aacggcggtg
     6421 atctgcggaa caccgacacc agtcacgata cgcgtcgtac agatagaacc agggccgata
     6481 cccactttaa cggcactgca accggcttct gccagcgcgc gggcacctgc agcagttgcc
     6541 acgttgccgc cgatgatttg caggtcagga tatttagcgc gagtttcacg gatacgctgc
     6601 agaacacctt cagagtgacc gtgagaggag tcgatcagca gaacgtcaac gcctgcggca
     6661 accagcgcat caacacgctc ttcgttacca gcgcccgcgc caaccgctgc acctacacgc
     6721 agacggcctt gttcgtcttt acacgcgttc ggtttacgtt ccgctttctg gaagtcctta
     6781 acagtgatca tgccgatcag gtggaattca tcatcaacca ccagcgcttt ttcaacgcgt
     6841 ttttcgtgca tttttgccag caccacttca cgggcttcgc cttcacgcac ggttaccaga
     6901 cgctctttcg gcgtcatgta aacgctaacc ggctggctca agtcggtcac aaaacgcacg
     6961 tcacgaccgg tgataatgcc gaccagttcg ttttcttcgg taacaaccgg gtagccagca
     7021 aagccgttac gctcggtcag ttctttcact tcacgcagcg tcgtagttgg cagtacggtc
     7081 tgcggatcgg ttaccacgcc agattcgtgt tttttcacac ggcgaacttc ttctgcctgg
     7141 cgttcaatgg acatgttttt gtggataaag ccgataccgc cttcctgagc cagagcaata
     7201 gccaggcgcg cttccgttac ggtatccatt gctgcggaaa gcatagggat attcagacga
     7261 atagttttcg tcagctgagt gctgaggtca gcagtatttg gcagaacggt agaatgagca
     7321 ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag cgatacgtag catgggcaat
     7381 atctcgacca gagtggttaa taaatattgc cgcggcatta tacagagcgt aaccgattgc
     7441 atctacccct ttttgcaaaa aatgcttgct atccccggag ggcgggttac tatcgactga
     7501 ataacctgct gatttagaat ttgatctcgc tcacatgtta ccttctcaat cccctgcaat
     7561 ttttaccgtt agccgcctga atcaaacggt tcgtctgctg cttgagcatg agatggggca
     7621 ggtgtggatc agcggcgaaa tctctaattt tacacaacca gcctccggtc actggtactt
     7681 tacgcttaaa gacgataccg cgcaggtgcg ctgtgcgatg ttccgcaaca gcaaccgccg
     7741 ggtcaccttc cacccacagc acgggcaaca ggttttagtt cgcgccaata ttacactcta
     7801 cgagccgcgc ggcgattatc aaattatcgt cgaaagtatg cagccggccg gtgagggatt
     7861 gctgcaacag aagtacgaac agcttaaagc gaagttgcag gctgaaggtt tgttcgatct
     7921 gcaatacaaa aaatcactcc cctcccctgc gcattgcgtt ggtgtgatca cctcaaaaac
     7981 cggtgctaca ctacatgata ttttgcatgt gttaaaacgt cgcgatcctt ctctgccggt
     8041 gatcatctac cccaccgccg tacaggggga tgatgcgccg gggcaaatcg ttcgtgccat
     8101 tgaactggcg aataagcgca acgagtgtga cgtgttgatc gttggccgtg gcggcggttc
     8161 gctggaagat ttatggagct ttaacgacga acgcgtggcg cgggcgattt ttgccagtcg
     8221 cattccgata gtcagcgccg tcgggcatga aaccgatgtg acgattgccg actttgttgc
     8281 tgatctgcgt gcgccaacgc cgtcagccgc cgctgaagtg gtgagccgaa atcagcaaga
     8341 attgctacgt caggtgcaat ctgcccgtca acggctggaa atggcgatgg actattatct
     8401 cgccaatcgc acgcggcgct ttacgcagat ccatcaccgc ttgcagcaac agcatccgca
     8461 gctccggctg gcacgccagc aaactatgct cgaacgtctg caaaagcgga tgagctttgc
     8521 gctggagaat cagcttaaac gcgcgggtca gcaacagcag cgattaacac ggcaactcgt
     8581 ccaccaaaat ccgcagtcgc gtattcatcg cgcgcaaacg cgcattcagc aactggaata
     8641 tcgtttagca gaaaccctgc gcgcacagct tagcgccacg cgcgaacggt ttggtaacgc
     8701 ggtaacacac ctcgaagcgg ttagcccgct gtcaacgctg gcgcgcggtt atagcgtcac
     8761 cagcgccgcc gatggcgcag tgttgaagca ggtgaagcag gtgaaagtgg gcgatacgtt
     8821 gaccacgcga ctgggagatg gcgtggtcgt cagtgaagtg agtgcggtga cgaaaacccg
     8881 caagccgcgt aaaaaaacct gatagcccgt tgcgtatcag gctttcggaa cctcatattg
     8941 atagggtggg caaggttatt ggaaacaggt tgcaaacccc ggcgttttcg caggctgaat
     9001 gccacagttg agactgccag agccttcctc aacaccacaa tagaacgatc cagccttatc
     9061 agtagataaa tacgggaagc ttatcgatga cggccatccc aatgtgctaa agccggagtg
     9121 taatgtttgt aattcggcac tgctcggcac gttattcata ctaccgcact gccgtgcgac
     9181 atcccattta gtggaatcta taacatgtgc cagcgcccag gtttcattat tcacattggg
     9241 cgttgctgct gcggtaaccc ctgacggcaa ctccgatttt aatagcgggc gatgcagcat
     9301 cttgccactt accgtggcgg tatccgccat atgaccccag aaatacgccg tggcgacatt
     9361 aggactggtc ggcacggtga aaatcgtgtc cagcgtcgcc gttattgtgg cgttatccgt
     9421 cagcgtcacc gtcaacggcg ttttatatcc tggcgtatta tcctgaccaa cggtaaaact
     9481 tgctgtaccg tctgctccag taactccatc gattgtattc ccgctatcag ccaacgtgac
     9541 tgccgcaccg gatgagggtt gtacagtcaa atcgtccatt gtatccacat cgccatacaa
     9601 cgtcgccccg ctattacgcg gcgtagcgtc accacgtttg aggataaacg aaatattcgg
     9661 cactggtttc ccgacactgt ctttcacggt aacgacaacg ggaacggcgt cgcctttttt
     9721 caccttcgcc gcctgcgtac tgctatccag tgccgtcgat gtcaacgtga cgctggagac
     9781 agtcgcacgc gggttaacca ggcacccttg atacgcggta ttcaccgtcc cccacgctga
     9841 accattattc agaccgacta cctgcggatt actgcctccc agccagttat ccagcgccca
     9901 ccatgtatac tgcgtagacc aaccgatttg tgtcgtcagc gtgttccccg gatagcgagc
     9961 atagagcgat tctaaatcat cactcaatgg ccgacgtgcc gcttcacaag ggctgagatt
    10021 gctgtcagcc ctctgcgttg cgttaaacac gggccagact tcattgttac tattgacctt
    10081 gctaacccct gatgtcgacg gcagctcttt ccacaattta ggtctttcaa agaccacacc
    10141 gttttctgcc gttaccgtat ccggcatatg accccagtag gccgccattg cggaatcagg
    10201 gctggtgaca acggtgaaga tcgcatcaag gacagtggtg gtttccgtat cacgtgccag
    10261 gatggcggta aacggtgttg ccaggccgac agtatcaggc tgttcaacat tgaatatcgc
    10321 cgtaccatcg ctgccggtaa aaccaagcca ttgcgtgccg tgtgaggcga gagtgaatgt
    10381 tgccgacacg ggtgccagtt ccgtcaatac catcacattt gctgccgtgt ttacaatggt
    10441 tcctgagcgg ctcatcgcat tatcacgcct gagcgttatc gcttcgcccg ccagcgggtt
    10501 gccatcacga tctttgacca taaccacaat tggcaatggg ttatcttttt tcactgacgc
    10561 gctaggtcgg ccgccattgc tgagggttct ggcgtcatcc agtgcggatg acgtcaatgt
    10621 tacgctaacg cctgccggat gcggctgcgc cagacagagc atcagtgcgt tggttgcaga
    10681 attcttcgcg atagctcctg ttctcaggct gacagtctga taagaccacg ttgttgtacc
    10741 cagtatggcc atatcataag cccaccagtt acccgcattc atcggtaacc caaggtcagt
    10801 cattattgcg ccgttcggat gatcactata caaatccaac agttccttac tgcgcggttg
    10861 attctcgagt gggcaatctg tagatgttaa attttgcaag ttgctcatat acgaccaaac
    10921 ttcgccgtta gaactaatcg ataagcctga gctggtttcg gttttcagca ggggtcgttt
    10981 gaaggtgacg cccgccgaac tggtaaaggt gtccggcata tgcccccaga atttagcact
    11041 cggtgaatct gggctggtaa tcaccgtgta gataacatcc agcgtggcga ctttcgttgt
    11101 atcacgcgcc agcgtcacgg ttaacggcgt cgccaacccg gtagtagagt actgcccaac
    11161 ctcaaaggtg gctgtgccat cactgcccgt cgttgcgaga gtttttgctc cacttgctgt
    11221 caacacgcta tccctactca ccggaaccaa tgccgttacg gtaagatcgt ctgcactcgt
    11281 gttaacggtt gctttcgcgc gatccagcgt cgtttcacgc gaaagggtaa agtcggcata
    11341 tggcatcggg ttcccggcgc tatcacgcac ggtcaccgtc aacggaatca gctcaccttt
    11401 cttcgctaca gcgctcggtc gtccatcatt gctgagcgat tttgctgcat cctgcgccgt
    11461 tgaggtaagc tctattgtga ctgcttccgt atgaggctct accaaacaca tcactatcgc
    11521 cgtatagctg gacgatgcct gaatcaactg accatttccc ggaacaaacg cctggttact
    11581 ccagctaccg cccgctaccg ccatcatgtc ataatcccac cagttccctg cagcagtcgg
    11641 caagccgagc accgttgcca gttgaccatt gggataatcg acatacagcc cctgcatatc
    11701 agtcgtcagc ggttgatacg cctcatcaca cccagccttg ctgacgtccg ctttctcagt
    11761 cgcggtcagg agcgaccaga cttcattatt ggccgttttc gccgtaccgc cagagagttc
    11821 ggctttgagg aaaggccgtt tgaaggtgac gccttcgctg ctggtaaagg tttccgccat
    11881 atggccccaa ttggtcgcca gcggcgtatc cgggctggtg gtgacggtaa agatcaccga
    11941 caaggtgtcc gacgacacgc cagtatcgtt taatagagca taaaagtcgg ttcttaaccc
    12001 agtggaagca tcctgtcgga gcgtcatcgt agtggttcca tctggccctg ttacaccata
    12061 caggatggtc gttgcactgg tatccaccgt cgcacccgcc gcagtcgtga ctgacacttt
    12121 ctgcgatgac gtcacattct gacgattgat cgagttcgcg tgtttcaggg caaacggcgt
    12181 attgccgact aaattcccct gcgcatcttt agtcgaaatg cggatcgaca cctcatcccc
    12241 cttcttcgct ttgacggcgc taagtgtgct gtcaatgctg acaaactgcc cggggtctga
    12301 ggcttccagc gtaatctggc tggcagtcat caacggcgtt gtacggcagg tttgtaacgc
    12361 cagcacggtg gatacgattt gcgaaactgc gccagaattt agttgcacca cattgtagta
    12421 acggctccct gtttgtgtat tactcggggt attggtgaga taactcatat ttaccggcca
    12481 tccctgtacc gtattcatcg cattacctct attggcgtca tacaaagact tcagaccatc
    12541 cagcgttggc atataatctg taccacaccc attcatgctg gtgtcagccg actgagtcga
    12601 ggtgaatctt gcccaacttt caccgttctc aaacgtgcta tatcgtgcat agttgttggt
    12661 aagctcgcca aacagcaacg gtcgcttata aaccgaacca tcggaggccg tcaccgtttc
    12721 ggccatatgt ccccaaaaat tcgcttttgg cgaatccggg ctggtcacga cggtaaagac
    12781 caccggtagc atcgccttga cgctgctgga cgtatccagt tttgccgtga gatcggttct
    12841 taacccggta ctgttatcct gttttagggt aaatgtcgtt gacccgtcgg caccggtcac
    12901 gccgtaaatg gtttcactcg ttgactggaa attatttcgc gtatttcccc acgcatccgt
    12961 caccgtcagc gcgcccacac tcatcgtcgc cgatgaatta gcccgactta cactgttggc
    13021 tcgcgtcagg ataaatgccg tattgcccac aaggttgcct tgcgcatctt tggtgatgac
    13081 gcgcaccacg gattcttcgc tttttttcgc ttttaccacg ttataggtgg tatccagcgt
    13141 caccggatct gccgcttcca gcaggatttg gctcaccgcg gctatcggcg cggtctggca
    13201 ggtcagcagc tccagcgtcg ttgatgacac tgccgcactc gtgccattgc tgagatctac
    13261 cgccttgtag ttacgctgct ccatgctgcc gttgtcggaa gtgttggata aatagcgcgt
    13321 tgccaccggc cagccctgca cggttttcat ggtgttgccc gcattattag caaacagtga
    13381 cgtcagaccg gcctgagtcg gaatatagtt ggttccacag ccgctgtacg tcgtggaaaa
    13441 acttgcataa ttaatgttaa acaacgccca gttttcattg tcttcagacg ttgatgttcg
    13501 cccagtctga taggccagtt ccttcaacaa cagcgggcgc ttaaataccg taccgtccgc
    13561 agcagtcacc gtttccggca tatgtcccca catttttgcc aggcggctat ccgggctggt
    13621 caccaccgta aagattgtgt cgatgctgga ttttttcgtc gcatcagagt aaagcattgc
    13681 cgtcagcgcc gttttggtgc cgtgagtatc cgggcgggta atattgagaa ttttgctgcc
    13741 gtcattgtcg gtcatggcgg tatacaccgt tgcagtgtcg ttcaacgtgg tttcatctgc
    13801 cagcccgcca ttgactacca ccggcgcaac cagcgcatcg ccgctgcccg ccacatgttt
    13861 ttcgccgcta cgggtatagc catcaccgcg attgagggta aatggcatat ccgctaacgc
    13921 gttgccctgt gcgtctttta ccgtcacttt aacctgtagc gtctcgcctt ttttcagcgt
    13981 tgccgcgcta ttcccggtat tccattgcgc tttatcaacc acctccagcg taatgctgct
    14041 ggcaggtttg ttcgccgtct tcaggcaact gacgtaaacc ggcgtatcgc cgccaatagt
    14101 ttgtgcgcca tcgttcagcg ctatcgtgaa aaagtgtgga gttttatctg ccggtgagtt
    14161 gctccagtaa ggctgacgct gtgttggcca accgtgcgcc gtttgcaccg cgttaccact
    14221 gttggcgtta tacagcgcac ttagctggga acgacgtggc aacatattgg tgtcgcagcc
    14281 acctttactg gtactggacg tattggcctg gctcacgcgc gcccagattt cgttattttc
    14341 ggtcagggtt ccgtccgggc tggcgacttc cgacgccagt ttaggccggg taaaggttga
    14401 tgaatcaacg gtgatcgttt catccatatg gccccacatc tgcgcgccgg taacgtccgg
    14461 gctggttggg gtagtaaaaa taacgctgta gttaacggta ttcggcaggt aggaatttac
    14521 cggcgttacg ctcagcaccg tttttagccc gacgccctgt ggttgcttaa tctcgaccgt
    14581 cgccacgccc cgggcatcgg ttattcccgc ataaaccttc gatgactgtg atgtgccgta
    14641 ttgcgtgtta tccatcgtga ttgcaccact gctcgggtca tcaaacccgc tcacctgccc
    14701 cgctcggtta taccccatac ctttggtaat ggtgaatgca gtaaagggta cagggatatt
    14761 attgagcgag ttaatcgtac gcacggtcat cacaatggtg tcacccactt tggcttgcgc
    14821 ttgggtgcca acagacacat catgatcggt gctgacttca acattcgcta ccatctcatt
    14881 acccgaacag gagaggtaat tcggcttaaa gccggaatag ctgtcgacgc tcccgttccc
    14941 cagatccacc gatgaatgcg tggcttgctc caccgcgctt aaatagccct gttgtgcagt
    15001 gggccagcca tattcagtac caatcgcgtt accgggatgg gctgaaaaca ggctttccag
    15061 tgagctctgg cgcggtatat gaccgacgcc gcattccgcc tgcatactgg tgttttgatc
    15121 gtagagcgcc cagtcttcgt tattttcccg cactgttccc aattcatgct cggtttcatc
    15181 cgccagcaac gggcgtttat acaaactgcc ggactcaata atgccgcgca tatggcccca
    15241 catccgcgct ttgtcgctgt ccgggctggt gattacggta aaaatcacat ctttggcatc
    15301 tgtggcgtta aaatcgctgc gcattcttgc cgtgatatgg gttttcaccc ccgcgccgcc
    15361 gggctggctc agggtgagcg aagcctggcc gttggcgtcg gtcatgcctt cataggtatg
    15421 tgcgtcgacc tggcgaaaat tactgccgcc agcaatctgt accggatggg cttcccaggc
    15481 agtgtcggtt tgattcttac gattgacgcc atcatccaga tgtagcgaga agtagtaata
    15541 cgccagcggt tgatcgttgt ttttcgtgtc ggtgatggcc agacgcatcg tcgcatcctc
    15601 gcccaccttc gctttcatcg cctgcgccgt gtcatcatag ttttctggcg tgaggacgat
    15661 tttcggctcg acgtcaggcg cgggcttatc cacgcagctc acgaggaacg tggtgctgtc
    15721 cggcttctgc gtttcgccac ggttagagac atctgccgca tggtgctgcc cggtaagccc
    15781 cgccagcgat gaccagtaat aatcgccctg catcggccag ccgagcacag tctgtaccgt
    15841 ggagggaatc accgtcgcca gtgcgccgaa gtggcgcatg cccggcagca cggtgcaatg
    15901 gctatctgcg ccgctccagg taaaggtgga ccaggtttca ttgtgatcga cgaccgtggc
    15961 attttcgtta ctgacttccg ccgccagttt tggtctactg aacgtgtaac catgggcttc
    16021 caccgtctcc gccatatggc cccacatcgt ggcctgcgcc acgtccgggc tggtgagcac
    16081 ggtaaagatc accgccgcct cactggtttg tgcaatgccg gaaatgccga ctaccagcgg
    16141 cgttttaaca ccggggccgt taggctgggt caccgtgatg gttgccgtac cgttagcatc
    16201 cgatgtgcca cgatactcgg ttaccgtcgt cgttaactcg gtgctatcca gcaccaccgg
    16261 cgcggtatta tttaccgcgc cctgacggtt ttgggcatct ttacgcttga taataaatgg
    16321 aatattaccc gcgacgttac cctgacaatc tttggtggtg acggtcagcg tgatagtgcc
    16381 gccaacggtg gtttttgccg cgccgagcgt ttcgttataa ccactggcgc tcaccgaaat
    16441 taaatcgccc tgcgcgggcg cgatattcag cgtcactttc tggtcagcca gcaccaccgt
    16501 cccctcatag ggataaccgg tcgaggtcag cagataaagc agtggaatgg tctgctgcgc
    16561 atcaatctgt ggccaggcca ccagcgaacg cccggccaga ccttgcgcgc aaaagctgcg
    16621 gttggcgatg ggctgtggat tgagcggcgt atccggcgtg gcggcatccg cccacaccag
    16681 actgacggtc ggcggcgtct ggttttccca gcgcagcgca ggtggtgtag aaaactggtc
    16741 gccttcactg tcctgaggat tagcaactat caggttagca gcgtcatcgt caacgctaaa
    16801 ttcattcggt ttagcagaaa acgccacatc atgagatgca gcaggatcaa gctgaacgct
    16861 tccctgataa acgggtacgc tggcgctatc ggcaatagga actgtaccgt taaactcgcc
    16921 tgtagaacca ggatatgtct gccattccac ctctgcccgt gcaaagtcag acagcataaa
    16981 ggctgccaac atgcccagaa gcacaatgat ttttcctggt ttcagacata ccgacatgta
    17041 gaaaactcca gcatgataaa caaaacgtca acccgttttg cttaac
//
LOCUS       DAOFSV010000093        16850 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.093, whole genome shotgun sequence.
ACCESSION   DAOFSV010000093 DAOFSV010000000
VERSION     DAOFSV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.093"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            663..1607
                     /gene="leuO"
                     /locus_tag="QBH27_003190"
     CDS             663..1607
                     /gene="leuO"
                     /locus_tag="QBH27_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDQ7015471.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1768..1806)
                     /locus_tag="QBH27_003191"
     CDS             complement(1768..1806)
                     /locus_tag="QBH27_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015472.1"
                     /translation="MKRNDKILHYRG"
     gene            1925..3649
                     /gene="ilvI"
                     /locus_tag="QBH27_003192"
     CDS             1925..3649
                     /gene="ilvI"
                     /locus_tag="QBH27_003192"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDQ7015473.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGARFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3652..4143
                     /gene="ilvN"
                     /locus_tag="QBH27_003193"
     CDS             3652..4143
                     /gene="ilvN"
                     /locus_tag="QBH27_003193"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDQ7015474.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            4155..4241
                     /locus_tag="QBH27_003194"
     CDS             4155..4241
                     /locus_tag="QBH27_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDQ7015475.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            4323..5327
                     /gene="cra"
                     /locus_tag="QBH27_003195"
     CDS             4323..5327
                     /gene="cra"
                     /locus_tag="QBH27_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDQ7015476.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            5929..6387
                     /gene="mraZ"
                     /locus_tag="QBH27_003196"
     CDS             5929..6387
                     /gene="mraZ"
                     /locus_tag="QBH27_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDQ7015477.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            6389..7330
                     /gene="rsmH"
                     /locus_tag="QBH27_003197"
     CDS             6389..7330
                     /gene="rsmH"
                     /locus_tag="QBH27_003197"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDQ7015478.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            7327..7692
                     /gene="ftsL"
                     /locus_tag="QBH27_003198"
     CDS             7327..7692
                     /gene="ftsL"
                     /locus_tag="QBH27_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDQ7015479.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            7708..9474
                     /gene="ftsI"
                     /locus_tag="QBH27_003199"
     CDS             7708..9474
                     /gene="ftsI"
                     /locus_tag="QBH27_003199"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDQ7015480.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            9461..10948
                     /gene="murE"
                     /locus_tag="QBH27_003200"
     CDS             9461..10948
                     /gene="murE"
                     /locus_tag="QBH27_003200"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDQ7015481.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATICFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVVVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            10945..12303
                     /gene="murF"
                     /locus_tag="QBH27_003201"
     CDS             10945..12303
                     /gene="murF"
                     /locus_tag="QBH27_003201"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDQ7015482.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLVNLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            12297..13379
                     /gene="mraY"
                     /locus_tag="QBH27_003202"
     CDS             12297..13379
                     /gene="mraY"
                     /locus_tag="QBH27_003202"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDQ7015483.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            13382..14698
                     /gene="murD"
                     /locus_tag="QBH27_003203"
     CDS             13382..14698
                     /gene="murD"
                     /locus_tag="QBH27_003203"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDQ7015484.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            14698..15942
                     /gene="ftsW"
                     /locus_tag="QBH27_003204"
     CDS             14698..15942
                     /gene="ftsW"
                     /locus_tag="QBH27_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDQ7015485.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            15939..>16850
                     /gene="murG"
                     /locus_tag="QBH27_003205"
     CDS             15939..>16850
                     /gene="murG"
                     /locus_tag="QBH27_003205"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDQ7015486.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEVGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKII"
BASE COUNT         3897 a         3987 c         4742 g         4224 t
ORIGIN      
        1 gtcattaaat cagctccaga tgaatgcgat atgcttttag agttactgga tacaaaaacg
       61 gatgtcaacc ctgaagcaat aaaaacgtca cgccattgtg agttcggcat ccgtaaaatt
      121 agctaattgt gctgcggtgg ttaaaacaag cgatattaat ttctgcttaa ccgccgatgc
      181 ttttcatcgg ttgacatatt tcagaataat tttttgcatc taatcaacga gggaaagggg
      241 gataaaatgc acgcgttaca aaacctatcc tgatgatttg tattgaatta tatgttttgc
      301 gaattttttg atattcattt ggtgaatatt attgatcaat taatgttaag aattaatgca
      361 ttaaacatat aaattaatta ttaaaaagca catttaatcc attttgtgga tgattgagta
      421 ttcgtggtag ttatgattag attgttttcg caacaaaaaa attatggatt attatgctgt
      481 agtaaacgac tctttgctcg gcagtggatt ctgtttttat cagaacccgt atctttatgt
      541 tttcctaatt ttactcattt tgctttttct tattttatat gcatgataaa tcatattctc
      601 gaggattatt tctctgcatt ccaataaggg aaagggagtt aagtgtgaca gtggagttaa
      661 gtatgccaga ggtacaaaca gatcatccag agacggcgga gttaagcaag ccacagctac
      721 gcatggtcga tctcaactta ttaaccgttt tcgatgccgt gatgcaggag caaaacatca
      781 cccgtgccgc tcatgctctg ggaatgtcgc aacctgcggt cagtaacgct gttgcacgcc
      841 tgaaggtgat gtttaatgac gagctttttg ttcgttatgg tcgtggtatt caaccgactg
      901 ctcgcgcatt tcaacttttt ggctcagttc gtcaggcatt gcaattagta caaaatgaat
      961 taccaggttc aggctttgag cctgccagta gtgaacgtgt atttcatctt tgtgtctgca
     1021 gcccgttaga cagcattctg acctcgcaga tttataatca cattgagcag attgcgccaa
     1081 atatacatgt tatgttcaag tcttcattaa atcagaacac tgaacatcag ctgcgttatc
     1141 aggaaacgga gtttgtgatt agttatgaag acttccatcg tcctgaattt accagcgtgc
     1201 cattatttaa agatgaaatg gtgttggtag ccagcaaaaa tcatccaaca attaaaggcc
     1261 cgttactgaa acatgatgtt tataacgaac aacatgcggc ggtttcgctc gatcgtttcg
     1321 cgtcatttag tcaaacctgg tatgacacgg tagataagca agccagtatc gcgtatcagg
     1381 gcatggcaat gatgagcgta cttagcgtgg tgtcgcaaac gcatctggtt gctattgcgc
     1441 cgcgttggtt ggctgaagag ttcgctgaat ccttagagtt acaggtatta ccgctgccgt
     1501 taaaacaaaa cagcagaacc tgttatctct cctggcatga agctgccggg cgcgataaag
     1561 gccatcagtg gatggaagag caattagtct caatttgcaa acgctaactg aatgcagaat
     1621 aggtcagaca tcaatgtctg gtttattctg cattttttat tgaatgtaga attttattct
     1681 gaatatttgt gctctctatt ttaggattaa ttaaaaaaat agagaaattg ctgtaagttg
     1741 taggattcag ccgatttatt atcaatttca tcctctgtaa tggaggattt tatcgtttct
     1801 tttcaccttt cctcctgttt attcttatta ccccgtgttt atgtctctgt ctgccaattg
     1861 cttaagcaag atcggacggt taatgtgttt tacacatttt ttccgtcaaa cagtgaggca
     1921 ggccatggag atgttgtctg gagccgagat ggtcgtccga tcgcttatcg atcagggcgt
     1981 taaacaagta ttcggttatc ctggaggcgc ggtccttgat atttacgatg cattgcatac
     2041 cgtgggtggt atcgatcatg ttttagttcg ccatgagcag gcggcggtgc atatggccga
     2101 tggtctggcg cgcgcgaccg gggaagtcgg cgtcgtgctg gtaacgtcgg gtccaggggc
     2161 gaccaatgcg attactggca ttgccaccgc ttatatggat tccattccat tagttgtcct
     2221 ttccgggcag gtagcgactt cgttgatagg ttatgatgcc tttcaggagt gcgacatggt
     2281 ggggatttct cgcccggtgg ttaaacacag ttttctggtt aagcaaacgg aagacattcc
     2341 gcaggtgctg aaaaaggctt tctggctagc ggcaagtggt cgtcctggac ccgtggtcgt
     2401 tgatttaccg aaagatattc taaatccggc gaacaaatta ccctatgtct ggccggagtc
     2461 ggtcagtatg cgttcttaca atcccacgac ctccgggcat aaagggcaaa ttaagcgcgc
     2521 tctgcaaacg ctggtggcgg caaaaaaacc ggttgtctac gtaggcggtg gggcaatcac
     2581 ggcgggctgc catctgcagt tgaaagaagc ggtggaggcg ttgaatctgc ccgttgtttc
     2641 ctctttgatg gggctggggg cgtttccggc aacgcatcgt caggcactgg gcatgttggg
     2701 aatgcacggt acctacgaag ccaatatgac gatgcataac gcggatgtga ttttcgccgt
     2761 cggggcacgt tttgatgacc gaacgacgaa caatctggca aaatactgcc caaatgccac
     2821 tgttctgcat atcgatattg atccaacctc aatttctaaa accgtgactg ctgatatccc
     2881 gattgtgggg gatgctcgcc aggtcctaga acaaatgctt gaactcctgt cgcaagaatc
     2941 cgcccatcaa ccgctggatg agatccgcga ctggtggcag cagattgaac agtggcgcgc
     3001 tcgtcagtgc ctgaaatatg acactcacag tgaaaagatt aaaccgcagg cggtgatcga
     3061 gactctttgg cggttgacta atggggacgc ttacgtgacg tccgatgtcg ggcagcacca
     3121 gatgtttgct gcgctttatt atccattcga caaaccgcgt cgctggatca actctggcgg
     3181 cctcggcacg atgggttttg gtttacctgc ggcactgggc gtcaaaatgg cgttgccaga
     3241 agaaaccgtg gtttgcgtca ctggtgacgg cagtattcag atgaacatcc aggaactgtc
     3301 taccgccttg caatacgagt tgcccgtact ggtggtgaat ctcaataatc gctatctggg
     3361 gatggtgaag cagtggcagg acatgatcta ttccggccgt cattcacaat cttatatgca
     3421 gtccttacca gacttcgtcc gtctggcgga agcctatggt cacgtcggga tccagatctc
     3481 tcatccgcaa gaactggaaa gcaaacttag cgaggcgctg gaacaggtgc gcaataatcg
     3541 cctggtgttt gttgatgtta ccgtcgatgg cagtgagcac gtctacccga tgcagattcg
     3601 cgggggcgga atggatgaaa tgtggttaag caaaacggag agaacctgat tatgcgccgg
     3661 atattatcag tcttactcga aaatgaatca ggcgcgttat cccgcgtgat tggccttttt
     3721 tcccagcgtg gctacaacat tgaaagcctg accgttgcgc caaccgacga tccgacatta
     3781 tcgcgtatga ccatccagac cgtgggcgat gaaaaagtgc ttgagcaaat cgaaaagcaa
     3841 ttacacaagc tggtcgatgt cttgcgcgtg agcgagttgg ggcagggcgc gcacgttgag
     3901 cgggaaatca tgctggtgaa aattcaggcc agcggttacg ggcgtgacga agtgaaacgt
     3961 aatacggaaa tattccgtgg gcaaattatc gatgtcacac cctcgcttta taccgttcaa
     4021 ttagcaggca ccagcgataa gcttgatgca tttttagcat cgattcgcga tgtggcgaaa
     4081 attgtggaag ttgctcgctc tggtgtggtc ggactttcgc gcggcgataa aataatgcgt
     4141 tgagaatgat ctcaatgcgc aatttgcagc ccaacatgtc acgttgggct ttttttgcga
     4201 aatcagtggg aacctggaat aaaagcagtt gccgcagtta attttctgcg cttagatgtt
     4261 aatgaattta acccatacca gtacaatggc tatggttttt acattttacg caaggggcaa
     4321 ttgtgaaact ggatgaaatc gctcggctgg cgggagtgtc gcggaccact gcaagctatg
     4381 ttattaacgg caaagcgaag caataccgtg tgagcgacaa aaccgttgaa aaagtcatgg
     4441 ctgtggtgcg tgagcacaat taccacccga acgccgtggc agctgggctt cgtgctggac
     4501 gcacacgttc tattggtctt gtgatccccg atctggagaa caccagctat acccgcatcg
     4561 ctaactatct tgaacgccag gcgcggcaac ggggttatca actgctgatt gcctgctcgg
     4621 aagatcagcc cgacaacgaa atgcgctgta ttgagcacct tttacagcgt caggttgatg
     4681 ccattattgt ttcgacgtcg ttgccacctg agcatccttt ttatcaacgc tgggctaacg
     4741 acccgttccc gattgtcgcg ctggaccgcg ccctcgatcg tgaacacttc accagcgtgg
     4801 ttggtgccga tcaggatgat gccgaaatgc tggcggaaga gttacgtaag tttcccgccg
     4861 agacggtgct ttatcttggc gcgctgccgg agctttctgt cagcttcctg cgtgaacaag
     4921 gcttccgtac tgcctggaaa gatgatccgc gcgaagtgca tttcctttat gccaacagct
     4981 atgagcggga ggcagctgcc cagttattcg aaaaatggct ggaaacgcat ccgatgccgc
     5041 aggcgctgtt cacaacgtcg tttgcgttgt tgcaaggggt gatggatgtc acgctacgtc
     5101 gcgacggcaa actgccttct gacctggcaa ttgccacctt tggcgataac gaactgctcg
     5161 acttcttaca gtgcccggtg ctggcagtgg ctcaacgtca ccgcgatgtc gcagaacgtg
     5221 tgctggagat tgtcctggcg agcctggacg aaccgcgtaa gccaaaacct ggtttaacgc
     5281 gcattaaacg taatctctat cgccgcggcg tgctcagccg tagctaaacc gcgaacaaaa
     5341 gtacgcgcca ggtgaatttc tctctggcgc gttgagtacg ggaatggaca tcaatatcct
     5401 taaagtaaat aagactattc ctggctatta ttgataaatg cttttaaacc cgcccgttaa
     5461 ttaactcacc tgctgaaatg cacaataatt aaatgatatt gacagcgcgt ttttgcatta
     5521 ttttgttaca tgcagagatg aattgccgat ttaacaaaca cttttctttg tttttgcgca
     5581 aacctgctgg catcaagcgc cacacagacg caacagggac tgttaaccag agaagatatg
     5641 tcctaaaatg ccgctcgcgt cgcaaactga cactttatat ttgctgtgga aaatagtgag
     5701 tcattttaaa acggtgatga cgatgaggga ttttttctta cagctattca taacgttaat
     5761 ttgcttcgca cgttggacgt aaaataaaca acgctgatat tagccgtaaa catcgggttt
     5821 tttacctcgg tatgccttgt gactggcttg acaagctttt cctccgctcc gtaaactcct
     5881 tttagtggga aattgtgggg caaagtggga ataaggggtg aggctggcat gttccgggga
     5941 gcaacgttag tcaatctcga cagcaaaggg cgcttatcag tgcctacccg ttatcgggaa
     6001 cagctgcttg agaacgctgc cggtcaaatg gtttgcacca ttgacattca tcacccgtgc
     6061 ctgctgcttt accccctgcc tgaatgggaa attatcgagc aaaaattatc gcgtctgtcg
     6121 agcatgaacc cggttgagcg ccgtgtgcag cgcctactgt taggtcatgc cagcgaatgt
     6181 cagatggatg gcgcaggtcg attgttaatc gcgccagtac tgcggcaaca tgccgggctg
     6241 acaaaagaag tgatgctggt tggacagttc aacaagtttg agctgtggga tgaaacaacc
     6301 tggcatcaac aggtcaagga agatatcgac gcagagcagt tggctaccgg agacttatcg
     6361 gagcgactgc aggacttgtc tctataaaat gatggaaaac tataaacata ctacggtgct
     6421 gctggatgaa gccgttaatg gcctcaatat ccgtcctgat ggcatctaca ttgatgggac
     6481 ttttggtcgc ggtggtcact cacgtctgat cctctcgcag cttggcgaag aggggcgttt
     6541 gctggcgatc gatcgcgacc cgcaggctat cgccgttgcg aagactattg atgatccgcg
     6601 cttctccatc atccacggac ctttctctgc gctgggcgaa tatgttgccg agcgcgatct
     6661 tatcggcaag atcgacggca ttctcctcga tcttggcgtc tcttcaccgc aacttgatga
     6721 tgctgaacgc ggcttttcct ttatgcgcga tggtccgctg gacatgcgta tggacccaac
     6781 ccgtgggcag tcagccgctg aatggctaca aaccgcagaa gaagccgata tcgcctgggt
     6841 attgaaaacc tatggtgaag agcgttttgc caaacgcatt gctcgcgcca ttgtcgagcg
     6901 taaccgcgaa cagccgatga cccgcaccaa agaactggcg gaagtcgtgg ctgccgcaac
     6961 gccggtgaaa gataagttta aacatcccgc gacccgtacc ttccaggcgg tgcgcatttg
     7021 ggtaaacagt gaactggagg agatagagca ggcgctaaaa agctcgctca atgtgctggc
     7081 cccggggggg cggctttcga tcatcagctt ccactcactt gaagaccgca ttgtgaaacg
     7141 ttttatgcgt gaaaacagcc gcggtccgca agttccggca gggttaccga tgacagaaga
     7201 gcagctcaaa aaactggggg ggcgtcagtt gcgagcacta ggtaagttga tgccgggcga
     7261 agaagaggtg gctgagaacc ctcgtgcccg tagttcagtt ctgcgtattg cagagaggac
     7321 gaacgcatga tcagcagagt gacagaagct ctaagcaaag ttaaaggatc gatgggaagc
     7381 cacgagcgcc atgccttgcc tggcgttatc ggtgacgatc ttttgcgatt tgggaagctg
     7441 ccactctgcc tgttcatttg cattattttg acggcggtga ccgtggtaac cacggcgcac
     7501 catacccgtt tactgaccgc tcagcgcgaa caactggtgc tggagcgaga tgctttagac
     7561 attgaatggc gcaacctgat ccttgaagag aatgcgctcg gcgaccatag ccgggtggaa
     7621 aggatagcca cggaaaagct gcaaatgcag catgttgatc cgtcacaaga aaatatcgta
     7681 gtgcaaaaat aaggataaac gcgacgcatg aaagcagcgg cgaaaacgca gaaaccaaaa
     7741 cgtcaggaag aacatgccaa ctttatcagt tggcgttttg cgttgttatg cggctgtatt
     7801 ctcctggcgc tggcttttct gctcggacgc gtagcgtggt tacaagttat ctccccggat
     7861 atgctggtga aagagggcga catgcgttct cttcgcgttc agcaagtttc cacctcccgc
     7921 ggcatgatta ctgaccgttc tggtcgcccg ttagcggtga gtgtgccagt aaaagcgatt
     7981 tgggctgacc cgaaagaagt gcatgacgct ggcggtatca gcgtcggtga ccgctggaag
     8041 gcgctggcta acgcgctcaa tattccgctg gatcagctct cagcccgcat taacgccaac
     8101 ccgaaagggc gctttattta tctggcgcgt caggtaaacc ctgacatggc ggactacatc
     8161 aaaaaactga aactgccggg aattcatctg cgtgaagaat ctcgccgtta ttatccatcc
     8221 ggcgaagtga ctgctcacct catcggcttt actaacgtcg atagccaagg gattgagggg
     8281 gtcgagaaga gtttcgataa atggcttacc gggcagccgg gtgagcgcat tgtgcgtaaa
     8341 gaccgctatg gtcgcgtaat tgaagatatt tcttctactg acagccaggc agcgcacaac
     8401 ctgacgctga gtattgatga acgcctgcag gcgctggttt atcgcgaact gaacaacgca
     8461 gtggccttta acaaggctga atctggtagc gccgtgctgg tggatgtcaa caccggtgaa
     8521 gtgctggcga tggctaacag cccgtcatac aacccaaata atctgagcgg cacgccgaaa
     8581 gaagcgatgc gtaaccgtac catcaccgac gtgtttgaac cgggctcaac ggttaaaccg
     8641 atggtggtga tgaccgcgtt gcaacgtggc gtggtgcggg aaaactcggt actcaatacc
     8701 gttccttatc gaattaacgg ccacgaaatc aaagacgtgg cacgctacag cgaattaacc
     8761 ctgaccgggg tattacagaa gtcgagtaac gtcggtgttt ccaaactggc gttagcgatg
     8821 ccgtcctcag cgttagtaga tacttactca cgttttgggc tgggaaaagc gaccaatttg
     8881 gggttggtcg gagaacgcag tggcttatat cctcaaaaac aacggtggtc tgacatagag
     8941 agggccacct tctctttcgg ctacgggcta atggtaacac cgttacagtt agcgcgagtc
     9001 tacgcaacta tcggcagcta cggcatttat cgcccactgt cgattaccaa agttgacccc
     9061 ccggttcccg gtgaacgtgt cttcccggaa tccattgttc gcaccgtggt gcatatgatg
     9121 gaaagcgtgg cgctaccggg cggcggcggc gtgaaggcgg cgattaaagg ctatcgtatc
     9181 gccattaaaa ccggtaccgc gaaaaaggtc gggccggatg gtcgctacat caataaatat
     9241 attgcttata ccgcaggcgt tgcgcctgcg agtcagccgc gcttcgcgct ggttgttgtt
     9301 atcaacgatc cgcaggcggg taaatactac ggcggcgccg tttccgcgcc ggtctttggt
     9361 gccatcatgg gcggcgtatt gcgtaccatg aacatcgagc cggatgcgct gacaacaggc
     9421 gataaaaatg aatttgtgat taatcaaggc gagggtacag gtggcagatc gtaatttgcg
     9481 cgaccttctt gctccgtggg tgccagacgc accttcgcga gcactgcgag agatgacact
     9541 cgacagccgt gtggctgcgg cgggcgatct ctttgtagct gtagtaggtc atcaggcgga
     9601 cgggcgtcga tatatcccgc aggcgatagc gcaaggtgtg gctgccatta ttgcagaggc
     9661 gaaagatgag gcgaccgacg gtgaaatccg tgaaatgcac ggcgtaccgg tcatctatct
     9721 cagccagctc aacgagcgtt tatctgcact ggcgggccgc ttttaccatg aaccctctga
     9781 caatttacgt ctcgtgggcg taacgggcac caacggcaaa accacgacta cccagctgtt
     9841 ggcgcagtgg agtcaactgc ttggcgaaac cagcgcggta atgggcaccg ttggtaacgg
     9901 cctgctgggg aaagtgatcc cgacagaaaa tacaaccggt tcggcagtcg atgttcagca
     9961 tgagctggcg gggctggtgg atcagggcgc gactttttgc gcaatggaag tttcatccca
    10021 cgggctggtt caacaccgtg tggcggcatt gaaatttgcg gcgtcggtct ttaccaactt
    10081 aagccgcgat caccttgatt atcatggtga tatggaacac tacgaagccg cgaaatggct
    10141 gctttattct gcacatcatt gcggtcaggc gattattaac gccgacgatg aagtgggccg
    10201 ccgctggctg gcaaaactgc cggacgcggt tgcggtatca atggaagatc atatcaatcc
    10261 gaactgtcac ggacgctggt tgaaagcgac cgacgtgaac tatcacgaca gcggtgcgac
    10321 catttgcttt agctcgagtt ggggcgatgg ggaaattgaa agtcgcctga tgggcgcatt
    10381 caacgtcagc aacctgctgc tggcgctggc gacactgctg gcactcggct atccgctggc
    10441 tgatttgctg aaaaccgccg cgcgtctgca accggtttgc ggacgtatgg aagtgttcac
    10501 tgcaccaggc aaaccgacgg tggtggtgga ttacgcgcat acgccggatg cactggaaaa
    10561 agccttacag gcggcgcgcc tgcactgtgc gggcaagctg tggtgcgtct ttggctgcgg
    10621 tggcgatcgt gataaaggca aacgtccact gatgggcgca attgctgaag agtttgctga
    10681 cgtggtggtg gtgacggacg ataacccgcg taccgaagag cctcgtgcca tcatcaacga
    10741 tattctggcg ggaatgttag atgccggata tgccaaagtg atggaaggcc gtgctgaagc
    10801 ggtgacttgc gccgttatgc aggctaaaga gaatgatgtg gtactggtcg cgggcaaagg
    10861 ccatgaggac taccagattg ttggcaatca gcgtctggac tactccgatc gcgtcacggt
    10921 ggcgcgtctg ctgggggtga ttgcatgatt agcgtaaccc ttagccaact taccgatatt
    10981 ctcaacggtg aactgcaagg tgcagatatt acccttgatg ctgtaaccac tgacacgcga
    11041 aaactgacgc cgggctgcct gtttgttgcc ctgaaaggcg aacgtttcga tgctcatgat
    11101 tttgccgacc aggcgaaagc tggcggcgca ggcgcactac tggttagccg tccgctggat
    11161 attgatctgc cgcagttaat cgtcaaggat acgcgtctgg cgtttggtga actggctgca
    11221 tgggttcgcc agcaagttcc ggcgcgcgtg gttgctctga caggttcctc cggcaaaacc
    11281 tccgttaaag agatgacggc ggcaatttta agccagtgcg gcaacacact ttatacggca
    11341 ggcaatctca ataacgacat cggtgtaccg atgacgctgt tgcgcttaac gccggaatac
    11401 gattacgcag ttattgaact tggcgcgaac catcagggtg aaattgcctg gactgtgagt
    11461 ctgactcgcc cggaagcggc gctggtcaac aacctggcag cggcgcatct ggaaggtttt
    11521 ggctcgcttg cgggtgtcgc gaaagcgaaa ggtgaaatct ttagcggcct gccggaaaac
    11581 ggtatcgcca ttatgaacgc cgacaacaac gactggttga actggcagag cgtaattggc
    11641 tcacgcaaag tgtggcgttt ctcacctaat gccgccaaca gcgatttcac cgccaccaat
    11701 atccatgtaa cctcgcacgg tacggaattt accctgcaaa cccctacagg tagcgtcgat
    11761 gttctgctgc cgttgccggg gcgtcacaat attgcgaatg cgctggcagc cgctgcgctc
    11821 tccatgtctg tgggcgcaac gcttgatgct atcaaagcgg ggctggtaaa tctgaaagct
    11881 gttccaggcc gtctgttccc cataaaactg gcagaaaacc agttgctgct cgacgactcc
    11941 tacaacgcca atgtcggttc aatgaccgcc gctgttcagg ttctggctga aatgccgggc
    12001 taccgcgtgc tggtggtggg cgatatggcg gaactgggcg ctgaaagcga agcctgccat
    12061 gtacaggtgg gcgaagcggc aaaagctgct ggtattgact gcgtgttaag tgtgggtaaa
    12121 caaagccatg ctatcagcac cgccagcggc gttggcgaac atttttccga taaaactgcg
    12181 cttatcgcgc gtcttaaatc actgattgct gagcaacagg taattacgat tttagttaag
    12241 ggttcacgta gtgctgccat ggaagaggta gtacgcgctt tacaggagaa tgggacatgt
    12301 tagtttggct ggccgaacat ttggtcaaat attattccgg ctttaacgtc ttttcctatc
    12361 tgacgtttcg cgccatcgtc agcctgctga ccgcgctgtt catctcattg tggatgggcc
    12421 cgcgtatgat tgctcatttg caaaaacttt cctttggtca ggtggtgcgt aacgacggtc
    12481 ctgaatcaca cttcagcaag cgcggtacac cgaccatggg cgggataatg atcctgacgg
    12541 cgattgtgat ctccgtgctg ctgtgggctt acccgtccaa tccgtacgtc tggtgcgtac
    12601 tggtggtgct ggtaggttac ggtgttattg gctttgttga tgattatcgc aaagtggtgc
    12661 gtaaagacac caagggcctg attgcccgtt ggaagtattt ctggatgtcg gtcattgcac
    12721 tgggtgttgc cttcgcgctg taccttgccg gcaaagacac gcccgcaacg cagttggtgg
    12781 ttccgttctt taaagatgtg atgccgcaac tggggctgtt ctacattctg ctggcttact
    12841 ttgtcattgt gggtactggc aacgcggtaa acctgaccga tggtctcgac ggtctggcaa
    12901 ttatgccgac cgtatttgtc gccggtggtt ttgcgctggt ggcgtgggcg accggcaaca
    12961 tgaactttgc cagctattta cacataccgt atctacgaca cgccggggaa ctggtcattg
    13021 tctgtaccgc gatagtcggg gcagggctgg gcttcctgtg gtttaacacc tatccggcgc
    13081 aggtctttat gggcgatgta ggttcgctgg cgttaggtgg tgcgttaggt attatcgccg
    13141 tactgctgcg tcaggaattc ctgctggtga ttatgggggg cgtgttcgtg gtagaaacgc
    13201 tttccgtcat cctgcaggtc ggctccttta aactgcgcgg acaacgtatt ttccgcatgg
    13261 caccgattca tcaccactat gaactgaaag gctggccgga accgcgcgtc attgtgcgtt
    13321 tctggattat ttcgctgatg ctggtcctga ttggtctggc aacgctgaag gtacgttaat
    13381 catggctgat taccagggta aaaatgtcgt cattatcggc ctgggcctca ccgggctttc
    13441 ctgcgtggac tttttcctcg ctcgcggtgt gacgccgcgc gttatggata cgcgtatgac
    13501 accgcctggc ctggataaat tacccgaagc cgtagaacgc cacacgggcg gtctgaatga
    13561 tgaatggctg atggcggcag atctgattgt cgccagtccc ggtattgcac tggcgcatcc
    13621 atccttaagc gctgccgctg atgccggaat cgaaatcgtt ggcgatatcg agctgttctg
    13681 tcgtgaagca caagcaccga ttgtggcgat caccggttct aacggcaaaa gcacggtcac
    13741 cacgctagtg ggtgaaatgg cgaaagcggc gggggttaac gttggtgtgg gtggtaatat
    13801 tggcctgcct gcgttgatgc tgttggatga tgagtgcgaa ctgtacgtgc tggaactgtc
    13861 gagtttccag ctggaaacca cttccagctt acaagcggtg gcggcgacca ttctgaacgt
    13921 gactgaagat catatggatc gctatccgtt tggtttacaa cagtatcgtg cagcaaaact
    13981 gcgcatttac gaaaacgcaa aagtttgcgt ggttaatgct gatgatgcct taacaatgcc
    14041 gattcgcggt gcggatgaac gctgcgtcag ctttggcgtc aacatgggcg actatcacct
    14101 gaatcatcag cagggcgaaa cctggctgcg ggtgaaaggc gagaaagtgc tgaacgtgaa
    14161 agaaatgaaa ctttccgggc agcataacta caccaatgcg ctggcggcgc tggcgctggc
    14221 agatgccgca gggttaccgc gtgccagcag cctgaaagcg ttaaccacat tcactggtct
    14281 gccgcatcgc tttgaagttg tgctggagca taacggtgtg cgctgggtta acgattcaaa
    14341 agcgaccaac gtcggcagta cggaagcggc gctgaatggc ctgcatgtag acggcacgct
    14401 gcatttgttg ctgggtggcg atggtaaatc ggcggacttt agcccactgg cgcgttacct
    14461 gaatggcgat aatgtacgtc tgtattgttt cggtcgggac ggcgcgcagc tggcggcgct
    14521 acgcccggaa gtggcagaac aaaccgaaac catggaacag gcgatgcgct tgcttgctcc
    14581 gcgtgttcag ccgggcgata tggttctgct ctccccggcc tgtgccagcc ttgatcaatt
    14641 caagaacttt gaacaacgag gcaatgagtt tgcccgtctg gcgaaggagt taggttgatg
    14701 cgtttatctc tccctcgcct gaaaatgccg cgcctgccag gattcagtat tctggtctgg
    14761 atctccacgg cgctaaaggg ctgggtgatg ggctcgcggg aaaaagatac cgacagcctg
    14821 atcatgtacg atcgcacctt actgtggctg accttcggcc tcgcggcgat tggctttatc
    14881 atggtgacct cggcgtcaat gcccataggg caacgcttaa ccaacgatcc gttcttcttc
    14941 gcgaagcgtg atggcgtcta tctgatcctg gcattcattc tggcgatcat tacgctgcgt
    15001 ctgccgatgg agttctggca acgctacagt gccacgatgc tgcttggatc tatcatcctg
    15061 ctgatgatcg ttctggtagt gggtagctcg gttaaagggg catcgcgttg gatcgatctc
    15121 ggtttgctgc gtatccagcc tgcggagctg acaaaactgt cgctgttttg ctatatcgcc
    15181 aactatctgg tgcgtaaagg cgacgaagta cgtaataacc tgcgcggctt tctgaaaccg
    15241 atgggcgtaa ttctggtgtt ggctgtgtta ctgctggcac agccagacct tggtacggtg
    15301 gtggtgttgt ttgtgactac gctggcgatg ttgttcctgg cgggagcgaa attgtggcag
    15361 ttcattgcca ttatcggtat gggcatttca gcggttgtgt tgctgatcct cgccgaaccg
    15421 taccgtatcc gccgtgttac cgcattctgg aatccgtggg aagatccctt tggcagcggc
    15481 tatcagttaa cgcaatcgtt gatggcgttt ggtcgcggtg aactgtgggg acaaggttta
    15541 ggtaactcgg tacaaaaact ggagtatctg ccggaagcgc acactgactt tattttcgcc
    15601 attatcggcg aagaactggg atatgtcggt gtggtgctgg cacttttaat ggtattcttc
    15661 gtcgcttttc gcgcgatgtc gattggccgc aaagcattag aaattgacca ccgtttttcc
    15721 ggttttctcg cctgttctat tggtatctgg tttagttttc aggcgctggt taacgtaggc
    15781 gcggcggcgg ggatgttacc gaccaaaggt ctgacattgc cgctgatcag ttacggtggt
    15841 tcgagcttac tgattatgtc gacagccatc atgatgctgt tgcgtattga ttatgaaacg
    15901 cgtctggaga aagcgcaggc gtttgtacga ggttcacgat gagtggtcaa ggaaagcgat
    15961 taatggtgat ggcaggcgga accggtggac atgtattccc ggggctggcg gttgcgcacc
    16021 atctaatggc tcagggttgg caagttcgct ggctggggac tgccgaccgt atggaagcgg
    16081 acttagtgcc aaaacatggc atcgaaattg atttcattcg tatctctggt ctgcgtggaa
    16141 aaggtataaa agcactgata gctgcgccgc tgcgtatctt caacgcctgg cgtcaggcgc
    16201 gggcgattat gaaagcgtac aaacctgacg tggtgctcgg tatgggcggc tatgtatcag
    16261 gtccaggtgg tctggccgcg tggtcgttag gcattccggt tgtacttcat gaacaaaacg
    16321 gtattgcggg cttaaccaat aaatggctgg cgaagattgc taccaaagtg atgcaggcgt
    16381 ttccaggtgc tttccctaat gcggaagtgg tgggtaaccc ggtgcgtacc gatgtgctgg
    16441 cgctgccgtt gccgcagcaa cgtttggctg gacgtgaagg tccggttcgt gtgttggtag
    16501 tgggtggttc ccagggcgca cgcattctta atcagacaat gccgcaggtt gctgcgaaac
    16561 tgggtgattc agtcactatc tggcatcaga gcggcaaagg ttcgcaacaa tccgttgaac
    16621 aggcgtatgc cgaagtgggg caaccgcagc ataaagtgac ggaatttatt gatgatatgg
    16681 cggcggcgta tgcgtgggcg gatgtcgtcg tttgccgctc cggtgcgtta acggtgagtg
    16741 aaatcgccgc ggcaggacta ccggcgttgt ttgtgccgtt tcaacataaa gaccgtcagc
    16801 aatactggaa tgcgctaccg ctggaaaaag cgggcgcagc caaaattatc
//
LOCUS       DAOFSV010000094        16786 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.094, whole genome shotgun sequence.
ACCESSION   DAOFSV010000094 DAOFSV010000000
VERSION     DAOFSV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.094"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            54..596
                     /locus_tag="QBH27_003206"
     CDS             54..596
                     /locus_tag="QBH27_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015487.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            696..1898
                     /locus_tag="QBH27_003207"
     CDS             696..1898
                     /locus_tag="QBH27_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDQ7015488.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2118..2900
                     /locus_tag="QBH27_003208"
     CDS             2118..2900
                     /locus_tag="QBH27_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDQ7015489.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2915..4120
                     /gene="rhmD"
                     /locus_tag="QBH27_003209"
     CDS             2915..4120
                     /gene="rhmD"
                     /locus_tag="QBH27_003209"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDQ7015490.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4177..5466
                     /locus_tag="QBH27_003210"
     CDS             4177..5466
                     /locus_tag="QBH27_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7015491.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPGQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5484..6287
                     /gene="yfaU"
                     /locus_tag="QBH27_003211"
     CDS             5484..6287
                     /gene="yfaU"
                     /locus_tag="QBH27_003211"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDQ7015492.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6328..6561)
                     /locus_tag="QBH27_003212"
                     /pseudo
     CDS             complement(6328..6561)
                     /locus_tag="QBH27_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6562..7557)
                     /locus_tag="QBH27_003213"
     CDS             complement(6562..7557)
                     /locus_tag="QBH27_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDQ7015493.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRDFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7750..8940)
                     /gene="glpC"
                     /locus_tag="QBH27_003214"
     CDS             complement(7750..8940)
                     /gene="glpC"
                     /locus_tag="QBH27_003214"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDQ7015494.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMDTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8937..10196)
                     /gene="glpB"
                     /locus_tag="QBH27_003215"
     CDS             complement(8937..10196)
                     /gene="glpB"
                     /locus_tag="QBH27_003215"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDQ7015495.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10186..11814)
                     /gene="glpA"
                     /locus_tag="QBH27_003216"
     CDS             complement(10186..11814)
                     /gene="glpA"
                     /locus_tag="QBH27_003216"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDQ7015496.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGAAVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12087..13445
                     /gene="glpT"
                     /locus_tag="QBH27_003217"
     CDS             12087..13445
                     /gene="glpT"
                     /locus_tag="QBH27_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDQ7015497.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13450..14526
                     /gene="glpQ"
                     /locus_tag="QBH27_003218"
     CDS             13450..14526
                     /gene="glpQ"
                     /locus_tag="QBH27_003218"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDQ7015498.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14729..15379
                     /gene="inaA"
                     /locus_tag="QBH27_003219"
     CDS             14729..15379
                     /gene="inaA"
                     /locus_tag="QBH27_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDQ7015499.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15433..15687)
                     /gene="yfaE"
                     /locus_tag="QBH27_003220"
     CDS             complement(15433..15687)
                     /gene="yfaE"
                     /locus_tag="QBH27_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDQ7015500.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15687..>16786)
                     /gene="nrdB"
                     /locus_tag="QBH27_003221"
     CDS             complement(15687..>16786)
                     /gene="nrdB"
                     /locus_tag="QBH27_003221"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDQ7015501.1"
                     /translation="DQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQLSFFWRPEEVD
                     VSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISIPELETWVETWA
                     FSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDELIEMTSYWHLL
                     GEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNA
                     KIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDLFVQAAQQEKDW
                     ADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIPWINTWLVSDNV
                     QVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
BASE COUNT         3864 a         4161 c         4570 g         4191 t
ORIGIN      
        1 tacaatcccg cagctttggg aattttgatt aattgcttgt gggaaagagg ataatgaaaa
       61 aaattgcgct agcaggtctg gccggaatgc tgttggtttc tgcatcggtc aatgcaatga
      121 gcatcagcgg ccaggcgggt aaagaataca ccaatattgg tgtcggtttt gggactgaat
      181 cgacgggcct ggcgttaagc ggtaactgga cacataacga cgacgacggt gatgccgcgg
      241 gcgtggggct ggggttgaat ctgcctctcg ggccattaat ggcgaccgtt ggcggaaaag
      301 gcgtttacac caacccgaac gatggtgatg aaggttatgc agcggcagtt ggcggtggtt
      361 tgcagtggca aattggtgac agcttccgct tgtttggtga gtattactac tctccggatt
      421 cgctctccag cggaattaaa agttatgaag aagcgaatgc tggcgcgcgt tacaccatta
      481 tgcgtccagt cagcattgag gctggctacc gttacctgaa cctggcaggt aaaaatggca
      541 atcgtgataa cgccattgct gatggtccgt atgttggggt aaatgccagc ttctgacgcc
      601 ttccggcgcg gcgttctgtc gcgccattca gtttcttact acgccctgtc tccctttgcg
      661 ttatagtgat tcgataacaa aagcaggaga taaccatgtt aaaagtggaa atgttatcca
      721 ccggggatga agtgttacac gggcaaatcg ttgacactaa cgctgcctgg ctggccgatt
      781 ttttctttca tcaggggctg ccattatctc gccgcaatac ggtgggggat aaccttgatg
      841 acttagtcac cattcttcgc gaacgtagtc agcacgccga tgtgttgatc gttaacggcg
      901 ggctgggacc gaccagtgat gatttaagcg cactcgccgc tgcgacagca aaaggtgaag
      961 gcctggtgct gcatgaagcc tggctcaaag agatggaacg ctatttccat gaacggggac
     1021 gagtaatggc accgagcaac cgtaaacaag cggagctgcc tgccagtgct gaatttatca
     1081 ataacccggt aggcactgcc tgtggttttg ccatacagct taatcgttgc ctgatgttct
     1141 ttacccccgg cgtaccgtca gaatttaagg tgatggtcga gcacgaaatc ctgccgcgcc
     1201 tgcgcgagcg tttttcttta ccacagccgc cggtttgcct gcgtttgact acctttggtc
     1261 gttcggaaag cgatctggca caaagcctgg acactctaca actgccgccg ggcgtaacaa
     1321 tgggctatcg ctcctcaatg ccgattatcg aactgaaact caccggaccg gcaagcgagg
     1381 aacaggcgat ggaaaaactg tggctggacg ttaaacgtgt tgccggacag agcgtgattt
     1441 tcgaaggcac cgaaggactg cccgcgcaga tcagtcgcga attgcagagc cgccagttca
     1501 gcctgacgct gagcgagcaa ttcaccggtg gtttattggc tttgcaactt tctcgcgcag
     1561 gtgctccatt gctggcgtgt gaagtggttc cttcacagga ggaaaccctg gcgcaaactg
     1621 cgcactggat tacagaacgg cgggcgaacc attttgctgg gttggcactg gctgtttctg
     1681 gtctggaaga tgagcatctc aactttgcac tagccacgcc agacggcact ttcgctttgc
     1741 gtgtacattt cagtgccaca cgctacagtc tggctattcg ccaggaagtg tgcgcaatga
     1801 tggcgcttaa tatgctacgc cgttggttaa acggccagga catcgccagc gagcatggct
     1861 ggattgaggt tgttgagtcc atgaccttat ctgtctgatt ccgccactct gtgccggttt
     1921 actgcaccgg cacattcccc ctccggcaaa atttgtccaa cctgttgtac tcaataagaa
     1981 aaaatccgtg cgctgattca caaatttgtg cgtaatttct ccagtccgct ggacgagaaa
     2041 cgggatttga tgcgcttcac aattttactc cccctgaaca acgagactga ttccagtatc
     2101 taagactgga gtccagtatg ttggaatcaa gcaaagttcc cgccctgaca cgggctattg
     2161 atattctcaa tttgattgcc cggattggcc cctgtagtgc tgcgacgatc attgacacat
     2221 tggggatccc taaaagcaca gcctatttgc tgcttaatga actcaggcgt cagcgttttc
     2281 ttagccttga tcaccaggaa aatttttgtt tgtggaccag gctggtggag ctttccggtc
     2341 atgcgttaag caagatggat ctccgggaac tggctcgacc gcgtttgacg caactgatgg
     2401 ataccaccgg gctgttatgt cacctgggga ttattgataa cggcagcgct tattacattt
     2461 tgaaagtgga gtcatcagcc actatcagcg tgcgttccca tgaaggaaaa agcctttcgc
     2521 tttatcgctc cggaataggt aaatgcctgc ttgcctggca acctgcagct gtacagcaaa
     2581 gtattatcga agggctggtc tgggaacggg cgacacccac aacgattact catccacaac
     2641 ggctctacga agaactggcg cgtattcgcc gccagggctg gagctatgac aacggcgaag
     2701 attacgccga tgtccgttgt gtcgctgcgc cggtgtttaa cgccaataac gagcttaccg
     2761 ctgcgatttc ggtggtcggt acccgcttac aaatcaatga agaataccgt gattatctgg
     2821 cgggcaaggc cattgcctgt gccagggata tttcacgttt gttgggatgg aaaagtccct
     2881 ttgacttaca ggcctcataa caatggagaa catcatgacc ctaccaaaaa tcaaacaggt
     2941 tcgcgcctgg tttactggcg gtgcgacagc agaaaaaggc gctggcggcg gtgattatca
     3001 cgaccagggg gcgaatcact ggattgatga tcatattgcc accccgatga gtaaataccg
     3061 cgaatacgag cagtcccgcc agtcatttgg cattaacgtt cttggcacgt tgattgttga
     3121 agttgaagca gaaaacggcc agaccggatt cgctgtttcg acagccggtg aaatgggctg
     3181 ttttattgtc gaaaaacatc ttaaccgttt cattgagggt aaatgcgtca gtgatatcaa
     3241 actgatccac gatcaaatgc tcaatgccac cctgtattac tccggctctg gtggcctggt
     3301 gatgaatacg atttcctgtg tcgatctggc tctgtgggat ctgttcggca aagtggtcgg
     3361 gcttccggtt tataaacttt taggcggcgc tgttcgtgat gagattcagt tctacgccac
     3421 aggtgcgcgt ccggatctgg caaaagagat gggctttatc ggtggcaaaa tgccgacgca
     3481 ctgggggcca catgatggcg atgcggggat ccgcaaagat gccgctatgg tcgcggatat
     3541 gcgtgaaaaa tgcggtgagg atttctggtt aatgctcgac tgctggatga gtcaggacgt
     3601 gaattatgcc accaaactgg cccacgcttg cgcgccctat aacctgaaat ggatcgaaga
     3661 gtgcctgccg ccacagcagt atgaaggtta tcgcgaactg aaacacaacg caccagcggg
     3721 gatgatggtc accagcggtg agcaccacgg cacactgcaa tctttccgta cgctttcaga
     3781 aaccggtatc gacattatgc agccggatgt tggctggtgc ggtggcttaa ccacgctggt
     3841 ggaaattgcc gcaatcgcca aatccagggg gcaactggtg gtgccacacg gttcgtctgt
     3901 ttactcccac catgcggtga tcaccttcac caatacgcca ttcagcgaat tcctgatgac
     3961 cagcccggat tgttcaacga tgcgtccgca atttgatccg attctgctta atgagccagt
     4021 accggtgaat ggtcgtattc ataaatcagt gcttgataaa cccggtttcg gcgtcgaact
     4081 caatcgtgac tgcaatttga aacgccccta cagccactaa tcactccggg cgttgctgtg
     4141 acaacggtaa cgcccacata ttctaaggag ctttttatga gcaccgcttt gcttgacgcc
     4201 gtggtgaaga aaaaccgcgc gcgtttaatt ccgtttatgt tggcgctgta tgtgctggcg
     4261 tttctcgacc gttcgaatat tggttttgcc aaacagacct accagattga taccgggctg
     4321 agtaatgaag cttatgcgct gggagcaggc attttctttg tggtatacgc gtttctgggg
     4381 gttccggcga atcttttgat gcgcaaactg ggggccagaa cctggattgg tacgacaaca
     4441 ctgctgtggg gatttctttc ggctgccatg gcatgggccg atactgaagc gaaatttctg
     4501 atcgttcgca ctctgcttgg tgctgcggag gccgggtttt tccctggtat gatttatctc
     4561 acttcgcaat ggtttccgca gcgtaatcgc gccagcatta tggggctgtt ctatatgggc
     4621 gcaccgctgg cgttaacact gggatcaccg ctttccggcg cgctgctgga gatgcatgga
     4681 tttatggggc atcccggctg gttctggatg tttgtgattg aaggattgct ggcagtcggc
     4741 gctggggtat tcacattctt ttggcttgat gacacaccgg ggcaggcacg ttttctgagt
     4801 aaacaagaaa aaacgttgct tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact
     4861 tctcgattga gcgatgcgct gcgtaatggg cgagtctggc aactggcgat tatctacctg
     4921 accattcaag tggcggttta cggattaatt ttcttcctgc cgacccaggt tgctgcattg
     4981 ctggggacaa aagtgggctt tacggcgtcg gtggtcaccg ccattccgtg ggttgcggcc
     5041 ttgtttggga cctggcttat tccgcgctac tccgataaaa ccggcgaacg gcgtaatgtc
     5101 gcagcgctga cattactggc ggcgggcatt ggtattggtc tctccgggct gctttctcca
     5161 gtactggcga tcgtagcact gtgtgttgca gccatcgggt ttattgccgt gcaaccggtg
     5221 ttctggacga tgccgacaca acttctttcc ggtacggcgc tggctgcggg gattggtttt
     5281 gtaaacctgt ttggtgcagt gggcgggttt attgccccga tcctgcgcgt gaaagcagaa
     5341 acgttatttt ccagtgatgc ggcgggatta ctgacgctgg cagcggtggc ggtcatcggt
     5401 tcgctgatta ttttcactct gcgtgtaaat cgcactgttg cgcagaccga cgtggcacat
     5461 cattaaatag tttaaggaac acgatgaacg cattattaac caatcccttt aaagaacgtt
     5521 tacgcaaggg cgaagtgcaa attggtctgt ggttaagctc aacgactgcc tatatggcag
     5581 aaattgccgc cacttccggt tatgactggt tgctgattga cggggagcat gcgccaaaca
     5641 ccattcagga tctttatcac cagctacagg cggtagcgcc ctatgccagc caacccgtga
     5701 tccgtccggt ggaaggcagt aagccgctga ttaaacaagt cctggatatt ggcgcgcaaa
     5761 ctctactgat cccgatggtc gatactgccg atcaggcacg tcaggtggtg tctgccacgc
     5821 gctatcctcc ctacggtgag cgtggtgttg gggccagtgt ggcacgggct gcgcgatggg
     5881 gacgcattga gaattacatg gcgcaagtca acgattcgct ttgtctgttg gtgcaggtgg
     5941 aaagtaaaac ggcactggat aacctggacg aaatcctcga tgtcgaaggg attgatggcg
     6001 tgtttattgg acctgcggat ctttctgcgt cgttgggcta cccggataac gccgggcacc
     6061 cggaagtgca gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg
     6121 ctggttttct ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact
     6181 ttgtcgctgt tggcgttgac acgatgctct acagcgatgc cctggatcaa cgactggcga
     6241 tgtttaaatc aggcaaaaat gggccacgcg taaaaggtag ttattgatat caaaggccca
     6301 tggggatcgg ctgtgggcct gtgttaatta gtggttattc gctgccagat cggcttcgct
     6361 tagctgagtg gccgcgagca cctgatcacg atcaatgccc tgttccagca tgcgcaaagc
     6421 aattttaatg gcttgttcat gcatgccttc ctgcaaacct tctaatttac cttcttgcca
     6481 gccttcaatc ttaccctcct gccagccaat ttgatgtcct tcctgccgta gccgttcagc
     6541 aatagtcatc aatctctcct tttaatgaca gttcggaata tcagcatcag tgagttgggt
     6601 tgccgccagc acaatctcac gttcaaagcc ctgctccagc atgcgcaagg caattttaat
     6661 ggcttgctca tgcatgcctt cctgcaaacc ttctaattta cctttctgca aaccctccaa
     6721 tttaccttgc tgcaaacctt ctaatttacc ttcttgccag ccttcaattt taccttcttg
     6781 ccagccaatt tgatgtcctt cctgccgtag ccgttcagca atagtcatta atctctcctt
     6841 ttgtagtggt gaacgttcgg caatctcctg aataaagcgg gtgaaacggg aggtatcgcc
     6901 gcattgcagc agataattaa acagtgtttg tagctggctg tcattagtga agtctctaac
     6961 caaaagcgtg gtgatcctgt cgaccatacc gattaaatcg cggtcgcgaa tatgcttttg
     7021 aatcagttcc agcagagcta tacgccgatg ttgcatgatc tcatcgtcag gtacgatggt
     7081 gatatccacc aacggaaaag cttcggtgta caactgccgg gcaagttgcg gatcgtcaaa
     7141 ctcatccagc cagttgagtg agtacgggta gggcgaggct tcgccatgat aaaacagcaa
     7201 tggcaccacc agcggaactc tgtcatagcc tttatccagg tgacgctgca tggcggcagt
     7261 ggcatagcgc attagccgaa aagccatatt cttttctgca gagctttgat gttcaatcac
     7321 gcagtagata tagccgtcac cctcgctggt ttccacggac cacaaaacat ccgagtagta
     7381 agcgcgtaaa cttttttcaa taaaactggt gggttccagg cgtaaggttt gcaggttgca
     7441 aagcttgcgc agcggctctg gtagatgtat ttccagaaaa tcccgtgcgg tttcgggtgt
     7501 gaacataaag gttttaaaca ccgcatcatg cggggaggag gttgttgatt cggtcatggt
     7561 tgtccgtcac ctgatattta tgatgacgcg acattacggt tatcccaggc ctttgatatc
     7621 tgccagtttc agcctttgcg agacgttttc agaggaattt tcactgcgtt gcaacgcata
     7681 cattgggcac ggaatcgtgt acgcaaaaat gcccggtaac aacatgctta ccgggcatca
     7741 gaaaggagtt taagccaacg cctgtgccag cagcgtaatc ggatgttcgc agcgaagact
     7801 tgtggacatc tcaatctgcc atttgcaggt ttcgcagtcg gtgaccacca gatctgcgcc
     7861 gctctcttct atctggcgga acagtggtgc gccgatggct tgtgaagtgg ggtagttctc
     7921 ttttttgaaa ccgtaagttc ccgcaatacc gcagcactgg gaatccagca ccgttaactc
     7981 aagccccggg attttacgca acagctccag ggtgtagagc gtccagccca ttttttccat
     8041 atggcacgga gtgtgataaa ccactttcag cggcagcggt ttcagcggta acgttttgcc
     8101 ttcgtccagc ttgcgccaca gccagcgggt cgccagttcg atatgatcgc gcaagccttt
     8161 gttgtcgaca ttcagcactt ctgggtattc gtcgcgcagg gcaaatgtac acgttgagga
     8221 ggtggcaatc accggaatgc cttttactcc cacagcttcg cggatcgact ctacattcgt
     8281 aattgcctgt ttgcgtgctt tatcggtaaa accgttggcg atcagcggta cgccgcagca
     8341 tttttcttta ctgagcagtt gtacgccggt atccatcgcg ttgagcactt taattaaatc
     8401 tttgcctagt tgcggatggt tgtagttaac gaagcagccg tgaaagaaag cgacctggtc
     8461 tttatactgc gcttgctgag ccgccacgct gcgataccag cgacggaacg tgccgaagga
     8521 gtatttcggt agcgtgcggc gatgatcgat ttttaacgcc gcatcaagca gctgacgcac
     8581 cggtttcagc gaggtagcgg tgttgacgat gggtgcgaac ggcgtggata cgctacccat
     8641 caggtcggta tgactcaaca caaaattacg cagcgacggg cgcgtggtgt catatttcgc
     8701 ccgcgcgcgc tggataatat cgccaatctt cacatcggac gggcaggcga cttcacaacg
     8761 tttgcagttg atgcaatatt tcagcgcctc gtcatacagt gcgccatctt tcaaacgcag
     8821 acgctcgcca tccggcccgg cttgttttgg ccccggataa cccggattca cccggctcac
     8881 cgggcaggcg gtggtgcaga cggtgcactt aatgcagttt tcgaagctgg tgtcgttcat
     8941 tgttggcctc ctgcgcgttg ggcaatctgt tgagcggcat gtaaagcact gacggcacaa
     9001 acaccgccgc cgcatccctg ggcgatggga tcaaatccgc ccagcaccga accgatggca
     9061 aacaggttct caatggtttg ccctgcctgc gacgggcgta gtgcctcatc agtggttaca
     9121 ccgaactgct gccacggctg cggcgcaaaa aaatcgccct tataccattc accccgcgta
     9181 gcggtttgta gcacatcaag gccgagaatc ggctcgcgaa tgccgtcacg ttccgctacc
     9241 agtccgccgc taaagaaact gccgctcgca agcaccgcaa atcgtgggcg cagcgggata
     9301 tcggcgtgat tgcgcgtcca gatttcattc actacaccat ttttacaggt cacttttttc
     9361 acttcatcgc ccggcatcca cacgccgccc tggcgcacaa actggcgctg taactggttt
     9421 tgcagacgaa tgcccagcac ggaaggcggc agcgttggca aaagcatcaa tgaacaaggt
     9481 agtttttcat tcaaccaatg ccacagtttg tcatcggcca ggccgaagca ggcgggcatc
     9541 aggatcattt cgcaggtatt ggcgaccgga ataagcgcat caagtaacaa cggccagttt
     9601 tcttcattat caaggaagcg ggcaatattc accgcccgga actcggtggc gttattgcgc
     9661 agcacatcca gttccggcag ttcaatttca gcggtttcaa ctttaagatc caattcgcgc
     9721 aatgaagccg ccgcaagatg cgcctgaaaa tccatcaggc cgctaattcc cactacacat
     9781 attttcttcg cgggcagcgg ccagacaggg acttctggcg aacttagcca ggttgagcgc
     9841 agagtgccga gcggcgtaat ccgctggtga gccagttcta cgctgccctg caattgcgca
     9901 ccgctttcag cgattaatgc ctgcgcctgg caagcgagat cgagcacgcg ttgaggcccg
     9961 agaagagtgt aaggatgggc aggtgcctgc tgacgcaaag attccagtcc actgtgaatg
    10021 tctgtcaccg gttgaccatc cggcagatgg ctcagcaaat ccagcgatcc agatgagaaa
    10081 tggagcgcgc tttgaccacg agtgacaatg gcacagcgca ggccgtgttt ttgcagttgc
    10141 aggccacaga gtaatccggc gaggccgccg cccataatga cagtatcaaa gcgcatcttt
    10201 ctgctccttc tccagaccac acaatccctg ataaacccag cgggtaaatt cgctttcgcg
    10261 cagtgcatct ccccaggcga tgggttgcac gcctttccag cgttcgttta ggaaggtgga
    10321 aagttgctca atagattgcg cggacgtcgt gacgttaaaa cgttgcagca gtccggcagc
    10381 gcggcaggcg cagagctcgc cctggcaggt gcccatcccc acacgggtac gacggcgtaa
    10441 atccagcagg ctattaacgt ttaaattttc taccgcgtac tgcacttcac ccgcagttac
    10501 cgcttcgcac tcacatacca ggctacggtg cagacggcct tcgctcagcc aggcaggcgt
    10561 gcgatcgcca tgacgataaa ccgcagaacc gcgcagcggg gcaggtaggg agatgacttt
    10621 acgcaaggta acttcagcgg gttcctgtga accaggaagt gccagatcgg cggtcgtaca
    10681 ggggcgcgtg ttgcccagtt tgcggcatac ggcatcggta gcccattccg ccatcagccg
    10741 ataggtcatc agtttgccac cggtgatggt gataaacccg tccagaccat cgcgttcagc
    10801 atggtcgagc agcacgatgc cacggctgac gttacgtccg ctcggatcgt catcgctggc
    10861 aaccagcggg cgtacgccag agtaggcccg caaaatgcgc gttttcgcca tcacgggggc
    10921 cagtttttcc ccttcacgca gcagaatatc aacttcttct gccgttactc gattatcgtc
    10981 aatctcgttg taatcaatac gtaaagaggt ggtgccaatc agcgaaatgg tatcgccagg
    11041 caccagaata tcggcgtcgg aaggtttgcg gcagcggttg atcacatgct gattaatgcg
    11101 gtgatccatg atcagcagcg atcctttcgc cgggaacatg cgaatgcgca gatcggcata
    11161 ttcggcaatg tgttgccccc agatcccggc ggcattaact acgacaggtg catgaagtgc
    11221 ctgaatttcg ccggtgagat ggttacgtac acgaacgccg cacaccgccg cgccttcacg
    11281 aatcagcccc gtgacttcat gagcggtaag gataacggca ccgtgttctt tggcatccag
    11341 catgtttgct gctgtcagac gaaatggatc aacggtgcca tccggaactt tcaccgcgcc
    11401 aatcagtgcc gggttaacgg caggttcgat aatgcgcgct tgctgcggat caatggcttc
    11461 tgcgctgatc cctgcttctt cgcaggcgcg gataaaagtg gcctggaagg agaggtcatc
    11521 ttccggcagg gtgataaaca ggccgttggt tggttctacg cagtgacgtg caatgcgttt
    11581 caggatctgg ttttcactaa tacattcgcg ggccgattcc gcatcggtta ccgcatagcg
    11641 cgcaccgctg tgcagcaggc cgtggttacg cccggtggca ccggttgcga tgtcgtggcg
    11701 ctcaaccaaa atcacgcgca gcccgcgcag ggcacagtcg cgggcaatcc cggctcccgt
    11761 tgcgccaccg ccaatgataa tcacgtcact tgattgcgag tcgcgagttt tcattgttat
    11821 ccctcagaag ttcgtttttt accatttagc catagtaaaa acatgaattg tttgatttcg
    11881 cgcatattcg ctcataattc gaaagtgaaa cgtgatttca tgcgtcattc tgaacatttc
    11941 aaccatcgta tttaataatg tgtgcggtaa ttcacattta atttatgaat gttttcttaa
    12001 catcgcggca actcaagaaa cggcaggttc tcccactgaa tcaggctgtt aatcataaat
    12061 aagaccacgg gccacggagg ctatcaatgt tgagtatttt taaaccagcg ccacacaaag
    12121 cgcgcttacc tgccgcggag atcgatccga cttatcgtcg attgcgctgg caaattttcc
    12181 tggggatatt ctttggctat gctgcttact atttggttcg taagaacttt gcgctcgcta
    12241 tgccttatct ggttgagcag ggattctcac gcggtgattt aggttttgcc ctttcgggga
    12301 tctcgattgc ttatggattt tcgaaattca tcatgggttc ggtatcggat cgctcgaatc
    12361 cgcgcgtttt cctgcccgca ggtttgattc tggcggcggc agtgatgttg tttatgggct
    12421 ttgtgccatg ggcgacgtcg agcattgcgg tgatgtttgt actgttgttc ctctgcggtt
    12481 ggttccaggg gatggggtgg ccgccgtgtg gtcgtactat ggtgcactgg tggtcgcaga
    12541 aagaacgtgg cggtattgtg tcagtgtgga actgtgcgca caacgtcggt ggtggtattc
    12601 cgccgctgct gttcctgctg gggatggcct ggttcaatga ctggcacgcg gcgctctata
    12661 tgcccgcttt ctgcgccatt ctggtggcac tgttcgcctt tgcgatgatg cgcgataccc
    12721 cgcaatcctg tggcctgccg ccgattgaag agtacaaaaa tgattatccg gacgactata
    12781 acgaaaaagc ggaacaggag ctgaccgcga agcagatctt catgcagtac gtgctgccga
    12841 acaaactgct gtggtatatc gccatcgcca acgtgttcgt ttatctgctg cgttacggca
    12901 tcctcgactg gtcaccgact tatctgaaag aggttaagca tttcgcgctg gataaatcct
    12961 cctgggccta ctttctttat gaatatgcag gtattccggg caccctgctg tgcggctgga
    13021 tgtcggataa agtctttcgt ggtaaccgtg gggcaaccgg cgtcttcttt atgacactgg
    13081 tgaccatcgc gactatcgtt tactggatga acccggcagg taacccaacc gtcgatatga
    13141 tttgtatgat tgttatcggc ttcctgatct atggccctgt gatgctgatc ggtctacatg
    13201 cgctggaact ggcaccgaaa aaagcggcag gtacggcagc gggttttacc gggctgtttg
    13261 gttacctggg cggttcggtg gcggcgagcg cgattgttgg ctacaccgtg gacttcttcg
    13321 gctgggatgg cggctttatg gtaatgattg gcggcagcat tctggcggtt atcttgttga
    13381 ttgttgtgat gattggcgaa aaacgtcgcc atgaacaatt actgcaaaaa cgcaacggag
    13441 gctaatggca tgaaattgac gctgaaaaac cttagcatgg cgatcatgat gagcactata
    13501 gtcatgggaa gcagtgcaat ggcggcggac agcaacgaaa aaatagtcat cgcccatcgc
    13561 ggtgccagtg gatatttgcc ggagcatacg ctgccagcaa aagcgatggc gtatgcgcag
    13621 ggagcggatt atctggaaca ggatttggtg atgaccaaag acgaccatct ggttgttctg
    13681 catgaccatt atctcgatcg tgttactgat gttgccgatc gtttcccgga tcgggcgcgc
    13741 aaagacggtc gttactacgc gatagatttc acgctggatg aaattaagtc gctgaaattt
    13801 accgaaggtt tcgatattga aaacggtaaa aaagtgcaga cttatccggg gcgtttccca
    13861 atgggtaaat ctgacttccg ggtgcacacc tttgaagaag agattgaatt tgttcagggg
    13921 ttaaaccact ctaccgggaa aaatatcggt atctatccag aaattaaagc gccgtggttc
    13981 catcatcagg aagggaagga tattgcggca aaaacgctgg aagtgctgaa gaaatatggt
    14041 tacaccggta aggacgacaa agtttatttg caatgttttg atgctgatga gctgaagcgt
    14101 attaagaatg agctggaacc taaaatgggt atggatctca atctggtaca gctgattgcc
    14161 tataccgact ggaatgaaac gcagcagaaa cagccggacg gaagctgggt taattacaat
    14221 tacgactgga tgtttaagcc gggtgccatg aaacaggtgg cggaatatgc tgatggtatt
    14281 ggtccggatt accatatgtt gattgaggag acatcgcagc cgggtaatat caaactcact
    14341 ggcatggtgc aagatgctca gcagaataaa ctggtagtgc atccttatac cgtgcggtca
    14401 gataaactgc ctgaatacac tactgatgtg aatcagttat atgatgttct gtataacaaa
    14461 gcgggtgtaa atgggctatt tactgacttc cctgataagg cagtaaaatt ccttaataaa
    14521 gagtaaatta actaccggat ggcacttatg tgctatccga taccactctt cattaaagtc
    14581 tgtgggttat ccttaattgc aattatttag agcgagatga cttgttcccg gcataagata
    14641 ctcgtcaata caacacgtat tattaagatt ttcctcagct aaacatctat agtcattgtc
    14701 gttaataaaa aacgacaggc gatagggtat ggcagtttca gcaaaacacg acgaatttaa
    14761 tcactggtgg gcaacagagg gcgactgggt tgaagagcct aactatcgcc gtaatggtat
    14821 gagcggggtg caatgcgtcg agcgcaacgg caaaaagctg tatgtaaagc gcatgacgca
    14881 tcatctgttt cattccgtac gttatccgtt cggccgacca acgattgtcc gtgaggttgc
    14941 ggtaattaaa gaactggaac gggccggtgt catcgtgccg aagattgttt ttggtgaagc
    15001 ggtgaaaatt gagggtgaat ggcgcgcgtt gctggtgact gaagatatgg cggggttcat
    15061 cagcattgct gactggtatg cccggcatgc agtatcgcct tattctgacg aagtacggca
    15121 agccatgttg aaagcagtgg cgctggcgtt taagaaaatg catagcgtta atcgtcagca
    15181 tggctgttgt tatgttcgcc atatctatgt gaaaacagaa ggtaaggcag aagctggatt
    15241 tcttgacctg gagaaaagtc gtcgccgctt gcgtcgtgat aaagcgatta atcatgactt
    15301 ccgccagctg gaaaaatatc tggagccgat ccccaaagcg gactgggagc aggtgaaagc
    15361 atattactac gctatgtaat cgttgtaggc cagataagac gtataagcgt cgtatctggc
    15421 gttaaaacca attcacatct cgatttcaat atcgcctttt gcccggcaac aacagggcaa
    15481 aatttccccc ggctgaataa aagctaacgg ttcggcaatc cagtccacct ggcctgcgac
    15541 cagtcgggtg cgacaggagc cgcagtaacc ttcgcggcac tggtactcaa ccgccacatt
    15601 gtgggattcc agcgccgcca gaagggaagg gtgttcatcc tggcacagca gttgtgtgcc
    15661 agtaatgcgc agggtaacgc gggccatcag agctggaagt tactcaaatc gtcggtgtcc
    15721 acttccgcgt caatctgccc gaccagataa gaactgactt ccacttcctg cggagcaacc
    15781 tgcacgttat cagacaccag ccaggtgttg atccacggga tcgggttgga gcgcgtctgg
    15841 aacggcagat ccaaaccgac tgcctgcata cggatattgg tgatgtattc aacgtactgg
    15901 cagagaatgt ctttattcag accaatcatc gaaccatcac ggaacagata atccgcccag
    15961 tctttctctt gctgtgccgc ctggacaaac aggtcatagc actcctgctt acattcttcg
    16021 gcaatttccg ccatctccgg atcatccgcg ccgctgcgca gcagattcag catatgctgg
    16081 gtgccggtca ggtgcagggc ttcgtcgcgg gcaatcaggc gaataatttt ggcgttacct
    16141 tccatcagct cacgttcagc gaatgcgaaa gagcaggcaa agctgacgta gaagcggatc
    16201 gcttccagcg cgttaacgct catcaggcag agatacagtt ttttcttcag ctcgcgcagg
    16261 ctaacggtta cggttttacc gttaacggtg tgggtgcctt cgcccagcag atgccagtag
    16321 ctggtcattt cgatcagttc atcgtaatag ctggagatcc cctccgcacg tttctggatc
    16381 tgctcgttgg taacgatatc gtcaaagaca acagacggat cgttaacgat attacggatg
    16441 atatgggtat aggagcggga atgaatcgtt tctgagaacg cccaggtttc tacccaggtt
    16501 tccagttccg ggatcgagat cagcggcagc agtgccacgt tcggactgcg accctgaatg
    16561 gaatccagca gcgtctgata tttcaggttg ctgataaaga tgtgtttttc gtgttcaggc
    16621 agtgcctggt aatcgatacg gtcgcgggag acgtcaactt cttccggacg ccagaagaaa
    16681 gagagctgct tttcgatcag cttttcgaag atgtcatatt tttgctgatc gtagcgagcc
    16741 acgttgaccg gctgaccaaa gaacattggt tctttgagct gatcat
//
LOCUS       DAOFSV010000095        16618 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.095, whole genome shotgun sequence.
ACCESSION   DAOFSV010000095 DAOFSV010000000
VERSION     DAOFSV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.095"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..4452)
                     /gene="mukB"
                     /locus_tag="QBH27_003222"
     CDS             complement(<1..4452)
                     /gene="mukB"
                     /locus_tag="QBH27_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDQ7015502.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVWKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQ"
     gene            complement(4452..5156)
                     /gene="mukE"
                     /locus_tag="QBH27_003223"
     CDS             complement(4452..5156)
                     /gene="mukE"
                     /locus_tag="QBH27_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDQ7015503.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5137..6459)
                     /gene="mukF"
                     /locus_tag="QBH27_003224"
     CDS             complement(5137..6459)
                     /gene="mukF"
                     /locus_tag="QBH27_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDQ7015504.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6456..7241)
                     /gene="cmoM"
                     /locus_tag="QBH27_003225"
     CDS             complement(6456..7241)
                     /gene="cmoM"
                     /locus_tag="QBH27_003225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDQ7015505.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEIGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7377..8156
                     /gene="elyC"
                     /locus_tag="QBH27_003226"
     CDS             7377..8156
                     /gene="elyC"
                     /locus_tag="QBH27_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDQ7015506.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(8133..9026)
                     /locus_tag="QBH27_003227"
     CDS             complement(8133..9026)
                     /locus_tag="QBH27_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDQ7015507.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLSMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFNRRNFDLASKSLLPWLA"
     gene            complement(9180..9926)
                     /gene="kdsB"
                     /locus_tag="QBH27_003228"
     CDS             complement(9180..9926)
                     /gene="kdsB"
                     /locus_tag="QBH27_003228"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDQ7015508.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9923..10105)
                     /gene="ycaR"
                     /locus_tag="QBH27_003229"
     CDS             complement(9923..10105)
                     /gene="ycaR"
                     /locus_tag="QBH27_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDQ7015509.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADEGKS"
     gene            complement(10157..11389)
                     /locus_tag="QBH27_003230"
     CDS             complement(10157..11389)
                     /locus_tag="QBH27_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDQ7015510.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDRGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDSKMHRQKGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            complement(11426..12412)
                     /gene="lpxK"
                     /locus_tag="QBH27_003231"
     CDS             complement(11426..12412)
                     /gene="lpxK"
                     /locus_tag="QBH27_003231"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDQ7015511.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKLVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQFLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12409..14157)
                     /gene="msbA"
                     /locus_tag="QBH27_003232"
     CDS             complement(12409..14157)
                     /gene="msbA"
                     /locus_tag="QBH27_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDQ7015512.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14194..16458)
                     /locus_tag="QBH27_003233"
     CDS             complement(14194..16458)
                     /locus_tag="QBH27_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDQ7015513.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWLHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKYRYHQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
BASE COUNT         4022 a         4633 c         4069 g         3894 t
ORIGIN      
        1 ctgagaaggc gcttcgtcac ttcctggaag cgtttcaggg agttgaggcg caaatcctcg
       61 caggccgacg acgtgaacgt gttcggtatt ctggaagact ttacgtacca gtttataggt
      121 ggtgcctttc tccgggctga tattttccgg cgctgcgata atgagctgca tttgcaaacg
      181 ttcacacaat tcaaacagcg tggcgatgga acgggcgtcc agtcgcgctg cttcatcgag
      241 gaacagcagg cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca
      301 gctttgtacc accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa
      361 tgcaccagac tctgcacgca accagccatc ggaaccacgg ttaacctcaa cttccatttc
      421 cagatagttg cggtaatcca gcagttcttc accgatggtc tgcggcgtgc gctgccccat
      481 atcaatctgc gggttaagac gttgatacag tttcgccagc gcttctgaga aggtcaggcg
      541 gttgctgtta aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat
      601 ggcgtgcgtt tcacgtacgt tcacgttcag acgtacgctg ttcacctggc caaacgatac
      661 gttctgcaac ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg
      721 aatgatgttc gccacgctgc gggaactgat cgccagtttc tgttcacgag aggttaattc
      781 ttcggtcaga cggctaagct cgatctccat ctgttcgata gcttccaccg gatcatcggt
      841 acgaataata tcctgacgaa tacgttcacg cagatgctga taaaccgcca cgaagaactg
      901 aattttacgt tccggacgtt ttggatcttc cgacatccgc agcacgtcgc gcagatgttc
      961 gttatccgcc accgccagac gcagcgcacc taacgcctta tccgacatgg agcgcaaatc
     1021 atcagcggag agataagcca gctcacgacg gtgtaagcga cgctcaacgc cattatcttt
     1081 caccatgcgc atcaccgcac accagcccgc tttggcggtc actacctgct cgcgcatctc
     1141 aaagtaatcc cgctccagct tgcgcagttt acgggtcagg ttgtccatct ccgcttcgca
     1201 gaaggtaagc gctttttcca gttgattgcg tcgtgaacgg ttattgctca gttgcgcgtg
     1261 caactcgtca cggcgaatac gtgcccgctc ttctgccccg ctatcagcac gcacgccgat
     1321 atcctgcaat tcacgttgca gatcgttaag tagctctttt ttggtgtcat acgaactttt
     1381 cagcgaagcc agcacctggt tgtactgatt caactgcgct gcgtgtccgc gcaacgcttc
     1441 acgagcgcgg gtacgctccg cttccgcctg ttccagacgt tcacgcagtt tttcgttgag
     1501 atcgctgtta ccgctaagca tttctgccga gtcagagtag ctaaagtgcg cacgacgctg
     1561 caccacttcc gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gctgagagta
     1621 cgcgtaatct tcttttaatt gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat
     1681 cggctccagt ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc
     1741 ttcatccaga cgttcgcgga tttcatcgac gcgatccgcc aggctgtcat cagccaacag
     1801 gttgagacgc ggcagaatgc ggttcagcgc cgtaacgccc tctttcgcct gctcaaactg
     1861 aatacgctgc tgctggttat cattttcatg attactcaac gcccgttcca gttcgacgcg
     1921 acggctgttc agttgacgga tttctgcttc cgggtcagac tcaaacgcca ccgccagatg
     1981 actgccgata aagcggctga acgcctgatg cagacgctgg gttttctgta catcaaagga
     2041 aagcgtggcg aagcgttcgg aaagcacttc ccgctcggca tggaggcttt caatacggct
     2101 ttcacgcgca gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg
     2161 atcggcgatt ttcaccacca ccgctttttc cagctcatca acgctgaaca cgctgtcatc
     2221 gaatgactgc ggatcccctt cgatcaaata gagatcttcc gggcaatcgg tcaagccttc
     2281 caggtgttcg gttacctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata
     2341 cagtgctgag aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag
     2401 cacaccacca aaacgctccg ccagcgcgtt cagacgctga tcttcagaac cacctggctg
     2461 gcttaaacgt tcgatctctt catcgacggc gtttttgcgc gcgcctactt catcgcgttc
     2521 aacaattgcc tcgcgctcac gctccagcaa ctgttgcaga aattcggtga catcctggct
     2581 ggaggtgaac tcttcgccgc actgttcgct caactggttg agactgtttt gcgctgccag
     2641 ccaaaccggc gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgctcctg
     2701 gcgcagtgcc atccgctctt ctcgggcgtt agagacgcta tcggaaagag aggcaatgcg
     2761 tgcttccagc tcctgatgca gagcttccag ttcatcaata tcaaaattct tgccctgacg
     2821 tttgcagaaa tctgccagca gacgctcagc ttcttgctgc tcacgcagac gctgttccag
     2881 ttcgcttaag cgcatccgca acggctgaac ctgctctgcc aggtgacgct gatcgacccc
     2941 ttcgcgcaat agttcgcgag cgacatccca cgcctcgtta cgcgccagcg ggccgttgat
     3001 tgccaccacc agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat
     3061 tttctgctca agagagagca ttttttcggt cgcttccagc tctttcgcct ggaaggtttc
     3121 cagccattcg gcagcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcacg
     3181 attaagcgca gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc
     3241 ctgctggtag tcagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc
     3301 acgagcctcg ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc
     3361 cagacggatc tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg
     3421 cagtgcggtt tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc
     3481 gccttcggca ccgttgtgct cagccagctc tcgcgccatg tcgacgtgtt tgtactgctc
     3541 agccgccagt tgctgacgcg aagtatgtag ctcgcgacga aactccaggg ccttatcgag
     3601 atgaacacgg cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtggcttc
     3661 gctgatcaga tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt
     3721 catgcggttt tcacgcagcg ccgcttccat gtcctggaac gctttccaca ccccactgtt
     3781 ttccggcaac aggtagtcgc gcagagaacg agtaatggcg ctggagatcc cgccatacag
     3841 cgaggcttcg atcagacgat agaatttact acggtcagac gctgagcgca gacgacgcgc
     3901 gatgatgccc agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa
     3961 ctgcacgcct tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc
     4021 ctggcgttcg ttcagagttt cggtcaccag ttgtgtcggc tgcaccgaca tcggcagtcc
     4081 ctgaatggca aacggcttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg
     4141 cacaccgacc accacgcgct ggtgacgcga gttaatggtg tcgagcatcg aataacagac
     4201 acccgctttc agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc
     4261 cgtagtgtta cggaaatgca gcagggtcag atcggggata agcgccgtaa cgaacgccgc
     4321 catggtggtg gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag
     4381 gtcaaaagtt cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc
     4441 gcgttcaatc attattcttc ctctccgcta tctggctgac tctcttcggt ttcatcgttg
     4501 agttgcagat gattttcaat cggcattgct tcaccatcac gaatcaggcg acgctgtgct
     4561 tcacggggat cgtcgccagc acgcacatcg gcgccgaagc ggaacaccga ttcggtaatg
     4621 cggaacttgc tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg
     4681 agcgaagaac gtactttttc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg
     4741 ttgttcacca gtttcagcag tttcgcttca tcggccaggg tgagcagttc gtcgtacagt
     4801 tcctgttgag taaaaatccc ctcattcgcc agccgttccg ggctgagata gagataacag
     4861 aggattttcc cgaccatcat atccagttcc gacaagacgg aacgagggat cagcgtggtg
     4921 gaacgtgggc gtagatagaa gaacccttcc ggtgcacgaa taagctcaac gttataacgc
     4981 gcgtaaaact cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg
     5041 tcgaggccaa tatggcggcc tgaacgtaag gcgctatcca gcgccggaaa taacggattc
     5101 gccagcgcct gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc
     5161 ctgtaccttg gctccgtaat cattaatcgg ctgccatttc gctggcagtc cggtgaaatc
     5221 tgcttgcgct acgccaagac gtaccgcctg atcaataaca atgcgcgcaa cgtcaaagtg
     5281 acgtgcacgc ggatactgtg acagatattc gcgtaccacc agaccaagat ccaacggcac
     5341 ttgtctggtt ttgtacacgg caagttgttc ttcgatgatc gccgccagct gttcgcggat
     5401 ctcgttaaac tcttcgtact ccagatcctc aggaagttcc ccagtcactt cttcatcgcg
     5461 cagtaccatc tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc
     5521 ccacggctca tcaaaatagg tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt
     5581 tttatccata tcgatcgcag tacgaataaa tttgtgtacg tggcggtcgt agccaatcca
     5641 caagtcgatg gattgctggc cccaactgat aatacggtcg agtttgctct gaagatcgaa
     5701 caccagacga tcgacaaaat gcaggtcgtc atgggtcatc gtcgcatcct gaatgcgtaa
     5761 caggttggcc tgcaatttat cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt
     5821 tccggaagtt tctgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt
     5881 caacaactgg gcgatatcat ccttcacctg ctgctgctgt tcatccatca gacgttgcgt
     5941 caggtcgata ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat aaacattgcg
     6001 gtgccagtga aattcatcac cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc
     6061 cgccacaatc gacaattgca tagaaaggcg cagcgtagaa aactcgcgct gacgaatgta
     6121 atagtcagtg atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg
     6181 ctcgctggta aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttgttggc
     6241 gcgcacgccg atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc
     6301 caccagctcg ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc
     6361 cagcagaaaa gagagtcggt ctaccggcag cgagatggag aagtcatttt ttctggccca
     6421 ggcaaccagt tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt
     6481 ttgcgcgcgg tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa
     6541 cgcgtttcta attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcacgcaga
     6601 taatcatgaa acacgcgaac gcctgtctta cccagaattt gccaaccagc ttcttccagc
     6661 cacagataaa cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt
     6721 ttcggcattc ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa
     6781 ccatgcgcat tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg
     6841 gtctgtaata cgctgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga
     6901 tcaacgggcg tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg
     6961 ttgtcgctca caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa
     7021 agatcgcata aaatgacctg atgcccgcgc tcggccattt tgattgcggt ctgcccttct
     7081 ccaccgccag catccagcac acgcagtttt tgcgggccta tttctgccag cacgcgatcg
     7141 agatcctgcc acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg
     7201 gaaaactttt ccgcaatatc atcaaaattg cgatcctgca ttgctttctc caccggttgg
     7261 ctaagacgtt ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc
     7321 tggtgtaata tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc
     7381 tttttacact gaaaaaagtg attggcaata tgttgctgcc acttccgcta atgttgttga
     7441 ttattggcgc tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt
     7501 ttattagcat tgggtggctg gcgctcttgt tattgagttt gcaaccggta gccgaccgcc
     7561 tgctgcgtcc catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata
     7621 tcgtggtgct cggaggtggt tatacctgga acccgcagtg ggcgccgagc tctaatttaa
     7681 tcaataacag tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat
     7741 caaaactgat cttcacggga ggcgtagcta aaaccaatac ggtgagcaca gccgaagtcg
     7801 gtgccagagt tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc
     7861 caaaagatac cgaagaagaa gctgcagcag tgaaacaggc gattggtgac gcacctttcc
     7921 tgctggtgac ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt
     7981 taaatccgct accagccccg gcgaatcagt tggccatcga ctcgccgctt aatccgtggg
     8041 aacgggcaat cccttctccg gtttggttga tgcatagcga ccgcgttggc tatgaaacgc
     8101 ttggccggat ctggcagtgg ctgaaaggtt cgtcaggcga gccacggcaa gagtgatttt
     8161 gatgccagat cgaagttacg ccgattaaat cgtccggtgt tgaccagttg cgcaacttca
     8221 tcccataaca catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc
     8281 agataactcc agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat
     8341 tctcgcggtg cccaaagcat tagtcccggt ccgaccatcg ccagtaactg atcgctgcgc
     8401 gaatctttca gcatgctgcg taaacagaag ttaccgtgaa tcagtacaca attgtcgtta
     8461 aagccttcaa ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc
     8521 atcgatagac cggtgttatt gaactgattg agcgtggtcc atagcacttc aacatgttgc
     8581 cggtaccatg agggccagaa attttcctga gtattgtcga ccgcgccgac gcaaccacga
     8641 ctgtcctgac ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ttgttcccag
     8701 cgttctggtg ttcgggctgg tgcctccacc gaaacaccac gcattcgctc cagtagcagg
     8761 acatcggggc cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca
     8821 gtcccacttc ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta
     8881 ctaaagcttc ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac
     8941 gccgtatccg ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg
     9001 cttaattcgg cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata
     9061 atgagcgcca atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga
     9121 ggaaaatcgt aacggtacga cactcctccc aaaattggct ggagtgtcgt gaagtgaaat
     9181 tagcgcattt cagcgcgaac gcgctcgaga tcttcagggg tatccacacc tgtgccagga
     9241 acttcctggg cgacagcaac gtggattttt tcgccatacc acagaacacg aagctgctct
     9301 aacatttcga tatgttctaa cggacttggc tgccaggtaa cataacgacg gataaagcct
     9361 gctcggtagc cataaatacc aagatgacgc aggaagttat cgccaacggt ttcaaggtct
     9421 tttgcaaaac gatcacgatc ccacggaatg gtggcgcgag agaagtacag tgcgtaccct
     9481 tcggcatcga gaaccacttt caccgcattc gggttaaacg cttcttccgc attgtggatt
     9541 ggcaccgcca gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga
     9601 atgattgtcg cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg
     9661 ctgaatgcgc atttttcgac aacttccgcc aaacgttctg tacctgactg atgatcggcg
     9721 cgcgtcatac atacttcgcc gccagcggct tcaacagcgc gggcaacatc ctcatgatcg
     9781 gttgccacga tgatgcgctc ggcacctgat tcgcgcgcgc gttcaagaac atgaacaatc
     9841 atgggtttgc cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtaacgc
     9901 gcgggaataa tgaccacaaa actcatgatt taccctcatc agcagtcagc acgcgggctt
     9961 ccgtttccag caacactgga atgccatcgc gcagcgggaa agccaggttg tccagtttgc
    10021 aaataagctc ttgtttttcc tggttatacc aaagttttcc gttgcaaacc gggcaggcaa
    10081 tgatttcaag cagacgatga tccatagttc ctccagatgg atcgggttat gaatgcataa
    10141 atcttatcat aatcatttat gcgacggggt ctatttccca gccggcgcgg caatcagtaa
    10201 agaggccttg cgagcagcgt cctaatgtca cccgcgttgc ctgctgccag ttagcgaaat
    10261 cagtaatcgc ctgacgtaac cctttttgca gcgttgtcgt tggtttaatg ccttcctgta
    10321 accacagaga gataacttca aggatgcctt tctggcgatg cattttggaa tccattcgcc
    10381 caactaattg cccacgatgt aataacggca gaacaaaata gccatactgg cgtttcggcg
    10441 ctggggtata gcactccagc cggtagctaa aatcaaaaag ctgctccgcg cgtttgcgat
    10501 cccagacaac aggatcaaaa ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg
    10561 cgagcgcacg ctcgagtagc ggcagcaaat cagcatgcag ccaaagattg cccaattttt
    10621 caacatgcac agcaatgatt tgctgctgtt cagccctcgc ttcacgccac gccgccagtg
    10681 ccggacgttt tagccggtag taatctgcca gccactgttc gcgaaatatt cccaggctac
    10741 gcgcactgtt atccagcatg atgatttctg cttctgcttg cgaaacgaga tcgcgctcat
    10801 catcccagtc aggcatgaca cggtgggtta aatcataaac gcgctggaag ttgcgccgtt
    10861 caatcaccat cacctttccg gcagtaaata aaccttccag atgtcgttta tgcggcttcc
    10921 actcccacca gccgcttgca cctttacgag gatgctcaaa atcggctgaa cgtaccggcc
    10981 ccctatcatg aatatgctga attaacagtg caatttccgc cgcatgttcc tgcatccagg
    11041 cgtctttata tttccagccc attttttcag gtgccagcat gcggtggcga ataagacgaa
    11101 aatcgctacg cggcataaag caggcttcat gcgcccagta ttccattaat tcgccacgcg
    11161 ccagagactc atccagccac tgggcaggat aatttcccag acgactgaaa agcaccagat
    11221 atgggctacg ggcaacaata ttgatggtat cgatttgcag caaggacatg cgggagatcg
    11281 ttgccggaat atcctccaac gacgctcgac ggtggggttt gtttaacagg ccttgtgcag
    11341 cgagatgaag attacgcgca tcagcaaggg agaggtgcgg cagagacatt catgactcca
    11401 tcaatcgaac gctgccgcgg cgtaactagt tgccagaagc cagcgaagtt agttgcgcaa
    11461 gcagtttcgc tggttcatca cctgaaagct gtgcgtctac aggcaaatac caccaatttt
    11521 cttctgcaaa ggcccggcat ttcaccgcat ctttttcagt cattaccagc gtttgtccgg
    11581 cgcttaccaa cgcgctgaca tccgcatgat tcaaaaactg atgatcggcc agcggtacac
    11641 atttttcggg ttgtacgcca cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa
    11701 tccccgcaat cgccaccaca tgttcaagct gagcaacgtc acaacgtgta ccggtacgta
    11761 aattcaccgc ctgacccggc agcagatgca tggggatttc accgctgcgg gggacaccgc
    11821 cgttgacgat taccgcatca accaacttta agcgccctgc tcgctcacgc attggccccg
    11881 ccggcaacca ccagccattg ccaaagcgac gcacaccatc aataacgaca atttccacat
    11941 cacgcgccag acgataatgc tgtaaaccat cgtcagttac gatgatctgc acatcagggt
    12001 gttgcgccag aatggctttt accgcgtcag aacgcacggg agaaaccgca acaggcgcgc
    12061 cagtgcgttg ataaatcaac acaggctcat cacccgcctg tgctgtggtg gtatctgccg
    12121 acaataacag cggataagat tcagccttac caccatatcc ccgcgatacg acccccacgc
    12181 gaataccgcg ctgttgcagc tgttccacca gccagacaac gaccggggtt tttccgttgc
    12241 cgcctgcagt gagatttcca accacgacaa ccggtacggg ggcacgccag gcgcgcttca
    12301 gttttagttt atagcaaaga cggatcgcgc cactcaccag gccatacaac caggagagtg
    12361 gcagcaatag ccgccataaa ggggactcac cagaccagat tttttcgatc attggccaaa
    12421 ctgcattttg tgaagttgcg cgtaaacgcc acggtgctca agcaaatcgt tatgcgtacc
    12481 gcgttccaca atgacaccat cctcgacaac cacgatttcg tctgcctttt caatggtaga
    12541 caagcggtgg gcaatcacca gagaggtacg gtttttctgc aactcatcca gtgctgcctg
    12601 aatcgcacgt tcggattcgg tatccaacgc cgaggtagct tcgtccagaa tcagtatcgg
    12661 gctatcacgc aacaaggctc gagcgatagc aatacgctga cgctgaccgc cagagagcaa
    12721 cacgccgttt tcaccaatga ctgtatcgag accgttatcc atcttattga tgaagtccat
    12781 ggcgtaggcc atacgcgcag cttcttcaat ttgctcacgg ctgtactgtt cagtccttgc
    12841 gtaagcaatg ttgttggcaa ccgtatcgtt aaacagatgg acattctgcg acaccagagc
    12901 aacctggtta cgtaacgacg ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat
    12961 ttcgccttca tcaatatcgt aaaaacgtgt gattaggctg gcgatggttg atttacctga
    13021 accagagcgg ccaaccagag caaccgtctt ccctgccgga attttcaggt tgatgttacg
    13081 caatgcaggc acatcacgtc ccggataagt aaaggtgaca ttgcgaaact ccacgtcgcc
    13141 agtcgcacgc tcgatcacgc gcttaccttc gtctttctcc tgctcactgt ccagaatggt
    13201 aaacagcgtc tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagcga
    13261 tttcagcgga cgcataagtg caatcattga agagaaaaca acggtaatcg taccggcagt
    13321 caggctatcc atgacacttg ggaagcttgc cgcatacaga acaaacgcca gcgccaaaga
    13381 ggcgatcagc tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg
    13441 aagacgcatt cggttgctga ctttatcgaa gcgtttcgtt tccacttcct gaccaccgaa
    13501 aatcaatact tctttatggc ctttcagcat ttgttcggcg ctggtggtca cctgccccat
    13561 ggtgttctgc atgtttttac tgatgttgcg aaaacgcttc gatacaacgc gaatcgcaat
    13621 cgaaacaatc ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat
    13681 catgatgaac aggccgatga tcgacgcacc ttcacgcaca acagtaatca gtgcgccgga
    13741 agaagaagaa gcgacctgtt cggaatcgta ggtgatacgt gacaacagcg tacccgttga
    13801 ctgtttgtca aagaatgaaa ccggcattcc catcatgtga ccaaacaggc ggcgacgcat
    13861 agtcattacc acctttcctg atacccagga gatacagtag ctggagatat agctggtgat
    13921 accacgtaaa atcatcagcc cgatcaccac cagcggcatc cacatcagca cggagcgatc
    13981 tgttttacca aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct
    14041 ggctgcgttg aggattaacg ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat
    14101 ggttggccac agtcggcgga acgtctgcca cgtagagaga tctttgtcgt tatgcattca
    14161 aaaaaccagc atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa
    14221 ccactgatga taccaacgag gtaaaatttg ctctctgagg ctgctaatcc accagccttg
    14281 cgctgaaaaa ttgaccgtta tttgcccctg atgtggagta tcaagccact tataaccctg
    14341 ttgatgatag cgatatttaa ctttgcttga gggcagtcgc catgcgttat agcgcgatgc
    14401 cgatgcgagt gccacttttc cattcactcg ctgaattaat ggcaacgatg atgaggtatt
    14461 actgccatgg tgaggtacct gaagtaatgt tgcctgcatt tgctgccagt aacggcttag
    14521 catcttttgt tcagcggggg cttcaatatc accggttaga agaatgctat tcgtcccgtc
    14581 atcaatctta accacacagg aatggttatt tcctttatcg ttgctacctt gtaaaggcca
    14641 gtgcgcgctg aaacgcaatc cttgccattg ccacgcttcg ccacgcacac agggctgatg
    14701 atgttcccag tttaacggac ttcggatcca taacatcggc catgtgtgca atattgagtc
    14761 cagccctccc cggtgatcca gatgttcatg actcaaaata acgccttccg gttcaagatt
    14821 atgccagtgg agccagggga taatcagttg ttgcccacta tccccttcgg gccaggccag
    14881 tcccgtgtca tagagaatcg ctttgccgtt tcgcgcaatg accattgcca gcccctgccc
    14941 gacatcaagc atgtacactt gccactcgtc aggtcgaggt ttttgccaca gcggcaagca
    15001 catcagcaag cctacagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca
    15061 taccaccagt aagaaccatg gggaaaatgt tagccattgc caacgttcag caatgttgat
    15121 ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag
    15181 aaaccataac ccttgttcca gaataaacgg cccgcttaaa tgcacaacca tcgcggcgag
    15241 aatcaacgga accgtgataa atgtcaccag gggaattgca aaaagatttg caataaacga
    15301 ggtcagacta atgccatgaa atatgacaat ttgcacgggc ataagcagga gggtgattcc
    15361 cagttgcaga tggatgaggg aaacaaccgc gcgcaatacc ggcggcagct gccactccgg
    15421 acagggaaac cactgatacc aaaaaatcag tgccgcgacc gcagcggcag aaagccataa
    15481 actttgcgag agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca
    15541 tacatcccag ccactccatt gtcgtccact taacttaagc attccccaaa tggcaagcgc
    15601 caccacggta cgcaatgcag gaggttgcat tcccgtaagc caggcataaa aagcagcaca
    15661 gcagattcca ccaattaatg gcatttgcca gtggagccag cacccaggca gaaaaacttg
    15721 cccaccgcga atgagtcctg cagccagcaa cgccgcaaaa gcgatgtgca atcctgatat
    15781 cgccattaaa tgcgccgttc cagtatcacg catgatattt ttgatttctt tggggacgga
    15841 taaccgttcc cccataccta atccaagaat aaccgcgttc cacatatagg gttgcagcgt
    15901 tgtttgtagt gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc
    15961 ctgcagaaaa cggccggtga gcggttgatg ctgggcaatg gcataacgct ggctatcaaa
    16021 gccgccatca ttaagttgac catgaactgc acgaactttg agtttcattg accatacttg
    16081 tccggcacaa accgcttgcg gaagatattc gccatacagt acgaggcctg gcgcagggaa
    16141 tatacgtcga ccttgtagat gagtaatttg accgtaatga gtggtcatgc cgtcagtagc
    16201 aatgatctca acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat
    16261 ttgctttgct gctaatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac
    16321 ggttttaaca gggataaatg ccagtacaca ggcgaagaga gtcagaaacg caagggttac
    16381 tgtccctggc aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc
    16441 gactgtcgtt attttcatgc accctccctg tttatagaaa gtgtgcaact ttgttgaacg
    16501 gttgtctggt gggagttttg cgttccccga ggcacgttca gaagaattta acgatagggt
    16561 gaaaactgct caaaactctg cgaggcagct tccagttcca gattagagaa atgaaaaa
//
LOCUS       DAOFSV010000096        16529 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.096, whole genome shotgun sequence.
ACCESSION   DAOFSV010000096 DAOFSV010000000
VERSION     DAOFSV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.096"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..5500
                     /locus_tag="QBH27_003234"
     CDS             <1..5500
                     /locus_tag="QBH27_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HDQ7015514.1"
                     /translation="GDDATANNNGKTIVDGKDSTGTEIAGNNAVVNQDGTLDVSGGGH
                     GIDITGDSATVDNAISNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIAGNNAVVNQ
                     DGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNG
                     GTGTQINGDDATANNSGKTIVDGKDSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGD
                     SAKVDNKGGMTVTDPDSIGIQIDGDKAIVNNDGDNAISNGGTGTQINGDEATVNNNGN
                     TTVDGKDATGTEIAGNNAVVNQDGTLDVSGGAHGIDITGDGAIANTTGAITVADTDSV
                     GIQVDGNAVTVSNDGDSTITNGGTGTQVNGNDAQVNNTGNTTVDGKNSTGTEITGNNG
                     TVNLDGGLIVTGGAYGVEIAGDSAALNNEGDISVSDEGSIGVLINGEKATVSNTGDVN
                     VSNNATGFDITTNEGNISLAGSMQVGDFSTGMDLNGNNNSVTLAAKDLNVTGQKATGV
                     NVSGDANTINITGNVLVDKDQTADNAADYFYDPSVGINVNGSDNNVTLDGKLTVVADS
                     ELTTRGSAGAIIFDGSQENITGLSIAGDGNTFRLNGGIQLVGEANKLTDGSTIASERK
                     GSGKVPLVSVDGKSSVHLNGDSTISGDVLLGNTQIIDLRNGAMLDVGADATINMQVDT
                     YERYAMSADHLIDASSGSQLVNNGDINLRSIGFAAITGEDSFGSNTGNLTLSQYLDGL
                     FANGGYGYLATKGASVVNTGTITTKVAEQESVINLGASLGFNDLVFYNAANSMTGLSA
                     KTHGYVLNENGGKIEMFGRGNVGMLAIDESTAENAGQITLDALWVDVNDTTTLRDNIG
                     NDARGYSVGMGVGTDAGSGPRKNATAVNQQSGVITVYNAGIGMAAYGAGNTVINQGTI
                     NLEKNGNYDSSLGVNSLIGMAAYNSGTAINDQTGVININADNGQAFYSTGNGVILNYG
                     TICVNTNCLTGNDYNDTDSYTSILYTGGDVITAQSETQSLTKKATISDKKDGNVVNSG
                     ALSGADIAISSGKLVNTSTGTINNAIIINGGALSNEGSVAKVTQNAGTFNNTGSVNSR
                     MTQTGGTFNNQQGGTVKNGSRLANSAIANNMGIWSLGDTSSGSNASMLEINNNAVFKN
                     SGDFILDNSKNAVHLNQSGTFYNTGKMLLSNSSHSGVLNFWGGNGRFINGGIADVTAK
                     SLAVSASDAGSSNAFFWNQSNGVVNFDKDSGVAVKFIHSNYVAQNDGTMNISGNNAIA
                     MEGDKNARLVNNGTINLGTSGTTDTGMIGMQLDSGTTADAVIENNGAINIYANNSFAF
                     SMLGSVGHLVNNGTVTIADGVTGSGLIKQGDSVNIEGVNGNNGNNSEVHYADYTLPDV
                     PGSSVSVAASTPSSDGSQNKLNGYVVGTSSDGSAGKLKVNNASLKGVSVNTGFTAGTS
                     STSVTFDNVVQGSNLTDAETITSTSVVWNAQGTTDASGNVDVTMTKNAYTDVATDSSV
                     NTVAQVLDAGYTNNELYSSLNVGTTAELNSALKQVSGSQATTVFNEARVLSNRFSMLS
                     DAAPEVANGLAFNVVAKGDPRAELGNDTQYDMMALRKSLTLTEHQNLSLEYGIARLEG
                     NGSDTAGDNGVTGGYSQFFGLKHQMAFDNGMNWNNALRYDVHQLDSSRSVAYGDINKT
                     ADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLEGGYQERNAGDFNLSMNS
                     GSETAVDSIVGLKLDYAGKDGWSANATLEGGPNLSYVKSQRTASVSGAGSQRFNIDDG
                     QNGGGFNSLATMGVKYSSQESALQVDAFHWKEDGISDKGVMLNFKKTF"
     gene            5720..6586
                     /gene="pdxI"
                     /locus_tag="QBH27_003235"
     CDS             5720..6586
                     /gene="pdxI"
                     /locus_tag="QBH27_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDQ7015515.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHLG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(6626..7231)
                     /gene="azoR"
                     /locus_tag="QBH27_003236"
     CDS             complement(6626..7231)
                     /gene="azoR"
                     /locus_tag="QBH27_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDQ7015516.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELITELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            7432..11334
                     /gene="hrpA"
                     /locus_tag="QBH27_003237"
     CDS             7432..11334
                     /gene="hrpA"
                     /locus_tag="QBH27_003237"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDQ7015517.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQQSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQAADEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            11607..12407
                     /locus_tag="QBH27_003238"
     CDS             11607..12407
                     /locus_tag="QBH27_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDQ7015518.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNAESVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            12617..14056
                     /gene="aldA"
                     /locus_tag="QBH27_003239"
     CDS             12617..14056
                     /gene="aldA"
                     /locus_tag="QBH27_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDQ7015519.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGENVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14098..15099)
                     /gene="gap"
                     /locus_tag="QBH27_003240"
     CDS             complement(14098..15099)
                     /gene="gap"
                     /locus_tag="QBH27_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDQ7015520.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            15288..15818
                     /gene="cybB"
                     /locus_tag="QBH27_003241"
     CDS             15288..15818
                     /gene="cybB"
                     /locus_tag="QBH27_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDQ7015521.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            16063..16236
                     /locus_tag="QBH27_003242"
     CDS             16063..16236
                     /locus_tag="QBH27_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015522.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(16308..16457)
                     /gene="hokB"
                     /locus_tag="QBH27_003243"
     CDS             complement(16308..16457)
                     /gene="hokB"
                     /locus_tag="QBH27_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDQ7015523.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         4256 a         3913 c         4474 g         3886 t
ORIGIN      
        1 cggtgacgat gcgaccgcga acaacaacgg caaaaccatt gtcgacggca aagattccac
       61 cggcacggaa atcgcgggta ataacgctgt agtgaatcag gacggtacgc tggacgtcag
      121 cggcggcggt cacggtattg atatcaccgg cgacagcgcc acggtggaca acgccatcag
      181 taacggcggc accggcacgc aggttaacgg tgatgaagcc accgtaaaca ataacggtaa
      241 aaccaccgtt gatggtcagg gctcgaccgg cacggaaatc gcgggtaaca acgcggtggt
      301 gaatcaggac ggtacgctgg acgtcagcgg cggcggtcac ggtattgata tcaccggcga
      361 cagcgccacg gtggacaaca agggcggtat gacggttacc gatccggatt ccatcggtat
      421 tctgatcgac ggtgataaag ccatcgtcaa caacgacggc gacaacgcca tcagcaacgg
      481 cggtaccggc acacaaatca acggtgacga cgcgaccgcg aacaacagcg gcaaaaccat
      541 tgtcgacggc aaagattcca ccggtaccga aatcgcgggc aacaatgcgg tggtgaatca
      601 ggacggcacg ctggatgtca gcggcggcgg tcacggtatt gatatcaccg gcgacagcgc
      661 caaagtggat aacaaaggcg gtatgacggt taccgatccg gattccatcg gtatccagat
      721 cgacggtgat aaagccatcg tcaacaacga tggcgacaat gctatcagca acggcggcac
      781 cggcacgcaa attaacggcg atgaagccac cgtgaataat aacggtaaca ccactgttga
      841 tggcaaagac gccaccggca cggaaatcgc gggtaacaac gcggtggtga atcaggacgg
      901 cacgctggat gtcagcggcg gtgctcacgg gattgatatc actggagacg gcgccattgc
      961 aaacacgaca ggagcgatca ctgtagcgga tacggactct gtcggtattc aggttgatgg
     1021 taatgcagtg accgtcagca atgacggaga cagcaccatt actaacggcg gtacgggcac
     1081 gcaggttaac ggcaacgatg ctcaggtcaa caatactggc aacaccacgg tagacggcaa
     1141 aaactccacc ggaacagaaa ttacgggcaa taacggcact gtaaatctgg atggtggtct
     1201 gattgtgacg ggtggtgcct atggcgtgga gatcgctggc gacagcgccg cgctaaacaa
     1261 tgaaggagat atttcggttt ccgatgaggg ctcaattggc gtactcatca acggtgaaaa
     1321 agcaaccgta tccaacacgg gtgatgttaa cgtcagcaat aacgcgacag gttttgacat
     1381 taccactaat gaagggaata tttcgctggc aggcagtatg caggtgggcg acttctctac
     1441 cgggatggat ctaaacggta ataacaacag cgtcacgctg gcggcaaaag atctgaacgt
     1501 caccggtcag aaggcgacag gcgtgaatgt atccggcgat gccaacacta ttaatatcac
     1561 cggtaatgtt ctggtagata aagatcaaac ggctgataac gcggcagatt atttctacga
     1621 cccgtctgtg gggattaatg tcaacggtag tgataacaat gtcactctgg acggtaagtt
     1681 aacggttgtt gctgacagtg aacttactac acgcggttct gctggcgcca ttatatttga
     1741 tggcagccag gagaatatca ccggactcag cattgcaggt gatggtaata ctttccgtct
     1801 gaatggcggt attcaactgg ttggtgaggc gaacaagcta actgacggtt ccactattgc
     1861 atccgagcgg aaaggcagtg gaaaggtacc tcttgtcagt gttgatggaa aatcgtccgt
     1921 tcatctgaac ggggattcca ctattagcgg tgatgtgctg ctcggcaata cacaaatcat
     1981 tgatttgcgg aatggtgcca tgctggacgt cggtgcggat gccacgatca acatgcaggt
     2041 tgatacttac gagcgttatg ctatgtcagc cgatcacctc attgatgcat cgtctggctc
     2101 tcagttggtc aataacggtg atattaatct ccgaagcatc ggttttgccg ctattactgg
     2161 tgaggattca ttcggaagca acactggcaa tctcacgctt tctcagtacc tcgatgggct
     2221 gtttgcaaac ggagggtatg gctatttagc caccaaaggg gccagtgtcg tgaacacagg
     2281 cactatcact acgaaagtgg cggaacaaga gagcgtaatc aaccttggag cgtcactggg
     2341 ttttaatgat ttagtctttt ataacgcagc gaacagtatg acgggcctta gcgccaaaac
     2401 tcatggttat gtacttaatg agaacggcgg caaaatcgag atgttcggac gtggcaatgt
     2461 cggtatgttg gccatcgatg aaagtacggc agaaaacgcg gggcagatta cactggatgc
     2521 actgtgggtt gatgtaaacg atacaacaac tttgcgtgac aacattggta acgacgccag
     2581 aggatattct gttggtatgg gcgttgggac agatgctggc agtggaccga ggaagaacgc
     2641 cacagcagtc aaccaacaga gtggtgttat caccgtttat aacgccggaa ttggtatggc
     2701 cgcctacggc gctggtaata cggttattaa ccagggcacc attaatctgg agaaaaacgg
     2761 taattacgat agtagcctgg gcgtgaattc gcttatcggt atggctgcat ataacagtgg
     2821 taccgccatt aacgatcaaa ccggtgttat caatattaat gctgacaatg ggcaggcatt
     2881 ttatagcact ggtaacggtg tcattcttaa ctatggcacc atttgtgtta acactaattg
     2941 cctgacgggc aatgactata atgatactga ctcctatacc tcgatattat acacgggtgg
     3001 cgatgtcatc actgcacaga gtgaaacaca aagtctgacc aaaaaagcca cgattagcga
     3061 caaaaaagac ggtaatgttg ttaacagtgg tgcgctttcc ggtgctgata ttgcgattag
     3121 tagcggtaag ttagttaata catcaacggg tacgattaat aacgcaataa ttatcaacgg
     3181 cggtgcgtta agtaacgaag gctctgtggc gaaggtgacg caaaacgccg gtactttcaa
     3241 taacacaggg tcggttaaca gtcgtatgac tcagacagga ggaacgttca ataaccaaca
     3301 gggcgggacg gtgaagaacg gctccagact ggccaacagc gctattgcta ataacatggg
     3361 gatctggtcc cttggcgata cgagtagtgg tagtaatgcc agcatgctgg aaattaataa
     3421 taatgccgtg ttcaaaaaca gtggtgactt cattctggat aatagcaaaa acgctgttca
     3481 tctgaatcag tcaggtacgt tctacaatac cgggaaaatg ttgctatcca actcttccca
     3541 tagcggcgta ctaaatttct ggggtgggaa tgggcgcttt attaacggcg gtattgcgga
     3601 tgtcacggcg aaatctttgg ccgtaagcgc cagtgatgcc gggagcagca atgccttctt
     3661 ctggaaccag agcaatgggg tcgtcaattt tgataaagac agcggcgttg cagtgaaatt
     3721 cattcacagc aattacgttg cgcagaatga cggcaccatg aatattagcg gtaataacgc
     3781 tattgcgatg gaaggggata aaaacgcacg actggttaac aacggcacga tcaacctcgg
     3841 aacgtcgggc acgaccgaca cgggcatgat cgggatgcaa ctggacagcg gcacgacagc
     3901 tgatgcggtc attgagaaca atggcgccat caacatttac gccaacaact cctttgcatt
     3961 tagcatgttg ggttccgtag gccacctcgt gaacaacggc acagtgacta ttgccgatgg
     4021 agtgacaggt tccgggctaa tcaagcaagg tgacagcgtt aacattgaag gtgtgaacgg
     4081 caataacggt aacaatagcg aagtgcatta tgctgattac actttgcctg atgtacctgg
     4141 ctcgtcggtt tctgttgcgg ccagcacccc gtccagtgac ggaagccaga acaaactgaa
     4201 tggttatgtt gtaggaacca gttctgacgg cagtgccggc aagctgaaag tgaacaatgc
     4261 aagcctgaaa ggggtttctg ttaacaccgg atttacggct ggaaccagct ccacctccgt
     4321 gaccttcgac aacgtcgttc agggcagtaa tctgaccgac gcagagacga ttacatcgac
     4381 ctctgtggta tggaacgcgc aaggcactac tgacgccagc ggcaacgttg atgtcacgat
     4441 gactaaaaat gcctacaccg atgtagcaac agattcatct gttaacaccg tcgcgcaggt
     4501 actggatgcg ggctatacca acaacgagtt gtacagcagc ctgaacgtag gcaccacggc
     4561 ggaactgaac agtgcgctga aacaggtcag cggcagccag gcaacaacgg tattcaatga
     4621 agcacgagtg ttaagtaacc gcttcagcat gttgtccgat gcagcaccgg aagtcgcaaa
     4681 cggtctggcc ttcaacgtgg tagcgaaagg cgacccgcgt gctgaactgg gtaacgacac
     4741 acagtacgac atgatggcgc tgcgtaaatc tctgacgctg actgaacatc agaacctgag
     4801 tctggaatac gggattgcgc gtctggaggg taatggttca gataccgcag gcgataacgg
     4861 agtgacaggc ggttacagcc agttcttcgg tctgaaacac cagatggcct ttgacaacgg
     4921 tatgaactgg aataacgccc tgcgttatga tgtgcaccag ctcgacagca gccgctcggt
     4981 agcgtacggc gatatcaata aaacagccga tgctaatgtg aaacaacagt acctggagtt
     5041 ccgtagtgaa ggggcgaaaa cctttgagct gcgtgaaggg ctgaacgtca cgccatatgc
     5101 cggggttaaa ctgcgtcaca cactggaggg tggctatcag gaacgcaatg cgggtgactt
     5161 taacctgagc atgaacagcg gtagcgaaac ggcagtggac agcattgttg ggctgaaact
     5221 ggactatgcc ggtaaggacg gctggagtgc gaacgccacg ctggaaggcg gcccgaacct
     5281 gagctatgtg aaaagccagc gaactgcgag cgtttcgggc gcgggaagcc agcgtttcaa
     5341 cattgatgat ggtcagaatg gtggtggttt caacagcctg gcgacaatgg gtgtgaaata
     5401 cagcagccag gaaagtgcgt tgcaggtaga tgcattccac tggaaagaag acggcatcag
     5461 tgataaaggt gtgatgctaa acttcaagaa aacgttctaa tttttagcat gtaatgcata
     5521 aaccgcaacg ctgatacagg ttgcggtttt tttattgccg gatgcggtgc ttgacgtgtc
     5581 ttaacctgcc tggggtttcg tgtctttcag ggcaacagta caactcatcc cgcaatcttg
     5641 tttacaccct cgcattattt atctcttttc gtttctatac tgatttttct taatccgttt
     5701 tattacaggg cagggtgcga tgagcagcaa cacatttact ctcggtacaa aatccgttaa
     5761 ccgtcttggt tatggcgcga tgcaactggc tggtcctggg gtttttggcc ccccaagaga
     5821 tcgccacgtc gctataaccg tgctgcgcga ggcgctggca ttgggcgtca atcatattga
     5881 taccagcgac ttttatggtc cgcacgtcac caatcagatt atccgcgaag cgctttatcc
     5941 ttactctgac gacctgacaa ttgtcactaa aattggtgcg cggcgtggag aggacgcatc
     6001 ctggttaccc gcattttctc cggcagagct gcaaaaagcg gtacacgata atctacgtaa
     6061 tctcggcctg gacgtactgg atgtggttaa cctgcgcgtt atgatggggg atggtcatgg
     6121 cccagcggaa ggatcgattg aggccagcct gacagtgctg gcggagatgc aacaacaagg
     6181 tctggtaaaa catcttggcc tgagtaacgt cacaccgacg caggttgcag aggcgcgaaa
     6241 gattgccgaa atcgtctgcg tgcagaacga atacaacatt gcccaccgtg ctgatgacgc
     6301 aatgattgat gctttggctc gtgatggcat tgcctacgtg ccgttcttcc cgctcggggg
     6361 ctttacgccg ctgcaatcct ccacgctttc cgatgttgct acaagcctta atgcgacacc
     6421 aatgcaggtg gcgctggcgt ggctgttaca gcgttcaccg aatattttgc tgattcctgg
     6481 cacctcttcg gtggggcatc tgcgggagaa tatggcgtcc gatgaattag tgctaacgga
     6541 tgacgtgctg gcaacgctgg acagtattgc gcaaaaggcg ggttaaataa aaaacctccc
     6601 actttcgtgg gaggcatcat aaatcttatg cagcaacaat actgtcgatg gctgcttttg
     6661 cgtcagactg tgctttcgct gccatttccg ggccgtacgc gatcccttcg gcgaagacaa
     6721 atttcacatc ggtaatgccg ataaagccca ggaatgtgga cagatatggc gttaccagat
     6781 ccgttgggcc atctttatgg atcccaccgc ggctggtgat aacgatggct tttttaccgg
     6841 tgaccaggcc ttccggaccc ttctcggtat agcggaaagt aacgcctgca cgtgcaacca
     6901 ggtcaaaata gtttttcaac tgggtcgaga tattgaagtt atacatcggt gccgcaataa
     6961 cgataacgtc gtgggctttc agctcggtaa tcagctcatc ggaaagcgcc agagcttcct
     7021 gctgacgcgg agtcagcggc gcatcgctcg gacgcagtgc accaaccagt tcaccatcca
     7081 ggaccggaat cggatttgca gccaggtcac gcacggtgat ttcatcagcg gagtgctttt
     7141 cgcgccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga
     7201 tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatat ttgaatgggc
     7261 aatgccccgt tgcttgttga cactttattc acaatcctgc cacagagata gcgcaataaa
     7321 tcgaagccta tgttcgaatt tattgaacaa cgcatagaaa gctgcgatgt ggtactctat
     7381 atctatcatt taaaagaaaa ttaatcaggc agcctactgc ccactaacgt tatgacagaa
     7441 caacaaaaat tgacctttac tgccttgcag cagcggctgg attcgttgat gctgcgtgac
     7501 agattgcgtt tttctcgccg tctgcacggt gtgaagaagg ttaaaaatcc tgatgcacaa
     7561 caggccattt tccaggagat ggcgaaagag attgaccatg cggcagggaa agtcctgctg
     7621 cgtgaagcgg cacgaccgga gattacttac ccggacaatt tgccggttag tcagaaaaag
     7681 caggacattc tcgaagcgat tcgtgaccac caggtggtga tcgtcgccgg ggaaacgggt
     7741 tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg gattaaaggg
     7801 ctgatcggcc atacccagcc gcgtcggctg gcggcgcgaa cggtggcgaa ccgtattgcg
     7861 gaagagctaa aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac
     7921 gtcagtgata acacgatggt caagctgatg accgacggta tcctgctggc ggagatccag
     7981 caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc
     8041 ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta
     8101 aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat
     8161 gcgccgatta tcgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt
     8221 gttgaagaag ccgatgatac cgagcgcgat cagttgcagg cgatttttga cgccgtagac
     8281 gaactgagcc aggaaagccc tggcgacatt ctgatcttta tgagcggcga gcgagaaatt
     8341 cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt
     8401 tatgcacggc tttccaacag cgaacagaac cgcgtgttcc agtcgcacag cgggcgacgc
     8461 attgtactgg cgaccaacgt cgcggaaacc tcgctgaccg taccgggtat taaatacgtt
     8521 atcgacccag gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg
     8581 ccgattgagc caatttcgca ggcttcagct aatcagcgta aaggccgctg tggtcgtgtg
     8641 tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctttcgcg cccggagttt
     8701 accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcactg
     8761 ggcttgggcg atatcgccgc attcccgttt gtcgaagcac cggataaacg caatatccag
     8821 gatggcgtgc gtctgctcga agaactgggc gcgatcacca ctgatgaaca gcaaagtgct
     8881 tataaactga cgccgctcgg tcgtcagctc tcgcagttgc ctgtcgaccc acgtctggcg
     8941 cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc
     9001 gcgctctcca ttcaggatcc gcgcgagcgt ccgatggata aacagcaggc agctgacgaa
     9061 aaacatcgtc gcttccacga caaagagtct gacttcctgg catttgtgaa tctgtggaat
     9121 tatcttggcg agcagcaaaa ggcgctttct tcaaacgcct tccgtcgcct gtgtcgtacc
     9181 gattatctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag
     9241 gtggtgaaag aacttggcat tccggttaac agcgaaccgg cggattatcg cgaaattcac
     9301 attgcgttgc tgaccggttt gctttcgcat atcggcatga aagatgccga taaacaagaa
     9361 tataccggcg cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag
     9421 ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt
     9481 gctgcgcgga tcgacccgga atgggtggag ccagttgctc agcatttgat taaacgcacc
     9541 tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact
     9601 gtttatggtt tgccgattgt tgccgcgcgc aaggtcaact acagccagat cgatccggcg
     9661 ttatgtcgtg aactctttat tcgccacgcg ctggtggaag gtgactggca gacgcgtcac
     9721 gcattcttcc gcgaaaacct gaaactacgg gcggaagtag aagagctgga acacaaaact
     9781 cgtcgccgcg atattctggt ggatgacgaa acgttgtttg agttctacga ccagcgcatc
     9841 agtcacgatg tgatctctgc acgccatttc gacagttggt ggaaaaaagt cagccgcgaa
     9901 acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc
     9961 agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagttat
    10021 cagtttgagc cgggggcaga tgctgacggc gtgaccgtac atattccgct gccgctgctt
    10081 aaccaggttg aggagagcgg gtttgagtgg cagatccccg gcctgcgccg tgagctgatc
    10141 atcgccctga ttaaatcgtt gccgaagccg gtacgccgta attttgtacc tgcgccaaac
    10201 tatgccgaag cgtttttagg ccgcgtcaca ccgctggaat tgccgttgct cgacagcctt
    10261 gaacgagagc ttcggcggat gactggcgtc accgttgacc gcgaagactg gcactgggat
    10321 caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta
    10381 aaagaagggc gctcattaca ggatctgaaa gacgccctga aaggcaaagt gcaggaaacg
    10441 ctgtctgccg tggcggatga cggtatcgag cagagcggtt tacatatctg gagttttggt
    10501 caactgccgg aaagctacga gcagaagcgc ggcaactaca aagtgaaggc ttggccggcg
    10561 ctggtggatg agcgtgacag cgtagcgatc aaactgtttg ataacccgct ggaacagaag
    10621 caggcaatgt ggaacggtct tcgccgttta ctgctgctaa atattccatc gccgatcaag
    10681 tacttgcacg aaaagctgcc aaacaaagcc aagctggggc tgtactttaa cccgtatggc
    10741 aaagtgctgg agctgatcga tgactgtatc tcctgcggtg tggatcaact gatcgacgcc
    10801 aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtccgtgct
    10861 gaactgaacg acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg
    10921 ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcgct ggggctttct
    10981 gacattaaag cgcagatggg cgggctggtg tatcgcggtt ttgtcactgg taacggcttc
    11041 aaacgactgg gcgacacgct gcgatatttg caggcaattg aaaaacgact ggaaaaactg
    11101 gcggtcgatc cacatcgcga ccgtgctcag atgctgaaag tcgaaaacgt ccagcaggcg
    11161 tggcagcaat ggatcaacaa actaccgcct gcacgtcgtg aggatgaaga cgtgaaagag
    11221 gtccgctgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact tggcacgcct
    11281 tatccgattt cggataagcg tattttgcag gcgatggagc agattagcgg ttaacactgc
    11341 tatttgcctg ataaatctaa aacccggtaa gcatttagcg ccgggttttt tattaattct
    11401 aaaaacgata agggaaaaaa ttcaggagtt cagaaaaata caattctctg ctgcaagatg
    11461 aataatgttt atctacagca tttccttaaa agatatgtca ggcttgcgga gtggcagttc
    11521 aggaaatacg acttcctcct ttcagagtgc tccgcttctc actattatct cacgcagtat
    11581 tcttaaggga acgataagga ggaaccatga atattacccc gtttccgacg ctttcgccgg
    11641 caaccataga tgccataaat gttatcggac agtggctggc gcaggatgat ttctccggtg
    11701 aggtgccgta tcaggccgat tgcgtgatcc ttgcaggcaa tgcggttatg ccgactatcg
    11761 atgcggcatg taagattgcc cgcgatcagc aaattccttt actgattagt ggtggtatcg
    11821 gtcactcgac aacttttttg tatagcgcca tcgcacagca tccgcactac aacactatcc
    11881 gcaccactgg cagagcagaa gctaccattc tggcggatat cgctcatcag ttctggcata
    11941 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
    12001 ttagcatcgc gttattgaat caggctgtag aacgagttca tacggctatc gttgttcagg
    12061 accccaccat gcagcggcgc acgatggcga cgttccgccg tataaccggg gataatcccg
    12121 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaga
    12181 gtgtaatctt tgttaatccg ttgcaaggat tatggccggt tgagcgttat ctatcgctac
    12241 tcactggcga gctgccgcgt ttacgcgatg atagcgatgg ctacggcccc cgcgggcgag
    12301 attttatcgt tcacgttgat tttccggcag aagtcattca ggcatggcaa acgctgaaac
    12361 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
    12421 gtgaaccact tgtttgcaaa cgggcatgat ttctgacttt tatttctgcc ttttatttct
    12481 gcctttaatt ccttttacac ttgtttttat gaagcccttc acagaattgt cctttcacga
    12541 tgccgtctct ctgatgattg atgttaatta acaataaatt taccaaaaac aaacatgcaa
    12601 atcacaggag tcgcccatgt cagtacccgt tcaacatcct atgtatatcg atggacagtt
    12661 tgttacctgg cgtggagacg catggattga tgtggttaac cctgctacag aggctgtcat
    12721 ttcccgcatt cccgatggtc aggccgagga tgcccgtaag gcaatcgatg cagcagaacg
    12781 tgcacaacca gaatgggaag cgttgcctgc tattgaacgc gccagttggt tgcgcaaaat
    12841 ctccgccggg atccgggaac gtgccagtga aatcagtgcg ctgattgtcg aagaaggggg
    12901 caagatccag cagttggctg aagtcgaagt ggcttttact gcggactata tcgattatat
    12961 ggcggagtgg gcacggcgtt acgagggcga gattattcaa agcgatcgtc caggagaaaa
    13021 tatccttctg tttaaacgtg cgcttggcgt gactaccggc attctgccgt ggaactttcc
    13081 gttcttcctc attgcccgca aaatggctcc cgctcttttg accggtaata ccatcgtcat
    13141 taaacccagt gaatttacgc caaacaatgc gattgcattc gccaaaatcg ttgatgaaat
    13201 aggccttccg cgcggcgtgt ttaaccttgt actcgggcgt ggtgaaaacg ttgggcaaga
    13261 actggcgggt aacccaaagg ttgcaatggt cagtatgaca ggcagcgtct ctgcaggtga
    13321 aaagattatg gcgactgcgg cgaaaaacat caccaaagtg tgcctggaat tggggggtaa
    13381 agcaccggct atcgtaatgg acgatgccga tattgaactg gcagtcaaag ccatcgttga
    13441 ttcacgcgtc attaatagtg ggcaagtgtg taactgtgca gaacgtgttt atgtacagaa
    13501 aagcatttat gatcagttcg tcaatcggct gggtgaagcg atgcaggcgg ttcaatttgg
    13561 taaccctgct gaacgcaacg acattgcgat gggaccgttg attaacgccg cggcgctgga
    13621 aagggttgag caaaaagtgg cgcgcgcagt agaagaaggg gcgagagtgg cgttgggtgg
    13681 caaagcggta gaggggaaag gatattatta tccgccgaca ttgctgctgg atgttcgcca
    13741 ggaaatgtcg attatgcatg aggaaacctt tggtccggtt ctgccggtgg tcgcatttga
    13801 cacgctggaa gaggctatct caatggctaa tgacagtgat tacggcctga cctcatcaat
    13861 ctatacccaa aatctgaacg tcgcgatgaa agccattaaa gggctgaagt ttggtgaaac
    13921 ttacatcaac cgtgaaaact tcgaagctat gcaaggcttc cacgctggat ggcgtaaatc
    13981 cggtattggc ggcgcagatg gtaaacatgg cttgcatgaa tatctgcaga cccaggtggt
    14041 ttatttacag tcgtagtgag tgaaagaggc ggaggttttt tcctccgcct gtgcgcgtca
    14101 gagtttagcg aatttttcga gggtgcgaat aagctgcgtg acgaagccat attcgttatc
    14161 gtaccaggcg accgttttca ccagttgtaa atcgcccacg gcggtaattt ccgtttgcgt
    14221 ggcatcaaac accgaaccga aatggctgcc aatgatatcg gaagagacta tttcttcatc
    14281 ggtataacca aatgactcgt tattgtttgt cgccttttta agcgcgttgt tcacctcttc
    14341 ggcagtcact ttttttccga gaatcgacac cagttcagtg accgaacctg ttttcaccgg
    14401 cacgcgttgc gcatgacctt tcagtttgcc gctcagttcc gggatcacca ggccaatggc
    14461 ttttgccgcc cccgtagtgt ggggaatgat attttctgcc gctgcgcgtg aagcacgtaa
    14521 atctttacca cgcgggccat ccaccagtga ctgggtgccg gtataggcat gaatggtcgt
    14581 catcgtgccg acttctatcc cgaaactgtc atgcaaggct ttggccatcg gcgcaaggca
    14641 gttagtggtg catgacgcca cggaaacaat ggtgtcgttg ccatccagag tgtcgtcatt
    14701 gacgttataa acgatggttt tcatttcacc ggcaggggcg gaaatcaaca ccttcttcgc
    14761 gccagcatca agatgcgcct gcgatttctc ggcggaggta taaaagcctg tacattcgac
    14821 aatgatttct gcgcctttcg ctttccacgg aatattttta gcctcttttt cggcgtaaac
    14881 cgcgatactt ttcccatcaa cgataagtga atcttccgta taatcaacgc tccaggggaa
    14941 cggtccgtag tttgaatcat gtttcagcag gtaggcgaga atttttgggg aagtgagatc
    15001 attaatagcg acaacgtcta tgttgctttt gacttcaagt aatcgacgca acaccagtcg
    15061 accgatacga ccaaaaccat taataccaac tttactcatg gtcttctcct gtcaggaacg
    15121 ttcggatgaa aattgatcct ttccaggctt agaccaggat ggcgggatgg gcaatctaca
    15181 ttcttgcagt gaaacgtaac gtactgaaaa cgggtgaaca atatttaatg aaattttaag
    15241 aaaagcccgt tatgttaacg gcaaattatg ttaaagcagg aaatgttatg ggaaataaat
    15301 actcaaggtt acaaatcagc attcactggc tggtcttttt gctggttatc gtagcgtatt
    15361 gcgcaatgga gtttcgtggt ttcttcccac gtagcgatcg gcccctcatc aacatgattc
    15421 atgtttcctg tggcatctca attctcgtac tgatggtggt tcgtcttttg ttaaggctga
    15481 aatacccaac cccgccgatt atacctaagc caaaaccgat gatgacggga ctggcgcatt
    15541 tgggccattt ggtgatttat ctcctgttta ttgcgctgcc ggtgattggt ttggtgatga
    15601 tgtataaccg gggcaacccg tggtttgcgt ttggtttgac gatgccttac gcttcagagg
    15661 ccaatttcga gcgggttgat agcttaaagt cgtggcatga aacgctggcg aatctgggat
    15721 attttgtcat cgggttgcac gctgcggctg cactggcaca ccactatttc tggaaggaca
    15781 acacacttct acgcatgatg ccacgtaaac gttcctgaag gatatttaaa gaaaacgcct
    15841 gtactaaaac cgacccgtgg tacaggcgaa gaatacgggc ctacatcgga agcgcctatt
    15901 atatttattt gtatgacaaa taaaaccata atccttgtct atacgtccat ctggcttatc
    15961 tttaatcaat tcacccgatc tttgatctca tcaacggtat caaaataaac cagcgtataa
    16021 cgttcaaatc gaacataaga ttcgataaat catggaggtt atatgaaaaa actggcactt
    16081 atcctggtta tgggaacgct tgtttccttt tatgccgatg ccgggcgcaa accctgttct
    16141 ggttcgaaag gggggatctc acactgtacg gcaggcggca aatttgtctg taatgatggt
    16201 tctattagtg catcgaaaaa aacatgcact aactgaagtg taaaaggggg gccatgagaa
    16261 aatgaatccg ctagtcagat gcgcggggaa aacccccgcg cttgccctta cctggacgtg
    16321 caggccatga gcgcagcaac ctccttatca ccgtcccgga accgcagttc gtagagcgtt
    16381 tgtcgggtca ggagtgtgaa tgtcagaatc gtaatgcaga taatgagcag acacaccacc
    16441 agagggttgt gcttcatagc ctttctcctt gccggatggc gggtaagagg ctaagatctg
    16501 aattgctagg ttcattcgtt ggcctcggt
//
LOCUS       DAOFSV010000097        16469 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.097, whole genome shotgun sequence.
ACCESSION   DAOFSV010000097 DAOFSV010000000
VERSION     DAOFSV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.097"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            37..1608
                     /gene="leuA"
                     /locus_tag="QBH27_003244"
     CDS             37..1608
                     /gene="leuA"
                     /locus_tag="QBH27_003244"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDQ7015524.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIDKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            1608..2699
                     /gene="leuB"
                     /locus_tag="QBH27_003245"
     CDS             1608..2699
                     /gene="leuB"
                     /locus_tag="QBH27_003245"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDQ7015525.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            2702..4102
                     /gene="leuC"
                     /locus_tag="QBH27_003246"
     CDS             2702..4102
                     /gene="leuC"
                     /locus_tag="QBH27_003246"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDQ7015526.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4113..4718
                     /gene="leuD"
                     /locus_tag="QBH27_003247"
     CDS             4113..4718
                     /gene="leuD"
                     /locus_tag="QBH27_003247"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDQ7015527.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(4825..4956)
                     /gene="sgrT"
                     /locus_tag="QBH27_003248"
     CDS             complement(4825..4956)
                     /gene="sgrT"
                     /locus_tag="QBH27_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDQ7015528.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5045..6700
                     /gene="sgrR"
                     /locus_tag="QBH27_003249"
     CDS             5045..6700
                     /gene="sgrR"
                     /locus_tag="QBH27_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDQ7015529.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLTEIADEPAGGLMLKGPQGEEQEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      6734..6833
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6864..7847
                     /gene="thiB"
                     /locus_tag="QBH27_003250"
     CDS             6864..7847
                     /gene="thiB"
                     /locus_tag="QBH27_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDQ7015530.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            7823..9433
                     /gene="thiP"
                     /locus_tag="QBH27_003251"
     CDS             7823..9433
                     /gene="thiP"
                     /locus_tag="QBH27_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDQ7015531.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQVLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            9417..10115
                     /gene="thiQ"
                     /locus_tag="QBH27_003252"
     CDS             9417..10115
                     /gene="thiQ"
                     /locus_tag="QBH27_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDQ7015532.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTLDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(10229..10993)
                     /locus_tag="QBH27_003253"
     CDS             complement(10229..10993)
                     /locus_tag="QBH27_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ7015533.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(11177..12664)
                     /locus_tag="QBH27_003254"
     CDS             complement(11177..12664)
                     /locus_tag="QBH27_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HDQ7015534.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALNFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGVVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            complement(12674..13111)
                     /locus_tag="QBH27_003255"
     CDS             complement(12674..13111)
                     /locus_tag="QBH27_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HDQ7015535.1"
                     /translation="MRIIPITPLFLLIFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTFDEVKALLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWFIFARVLLVAFPEGILF"
     gene            complement(13160..14113)
                     /locus_tag="QBH27_003256"
     CDS             complement(13160..14113)
                     /locus_tag="QBH27_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HDQ7015536.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYQNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLIAKIKG"
     gene            complement(14168..15091)
                     /locus_tag="QBH27_003257"
     CDS             complement(14168..15091)
                     /locus_tag="QBH27_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDQ7015537.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFANFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEVVLKQIADAADFQRQQLKY"
     gene            complement(15117..16349)
                     /locus_tag="QBH27_003258"
     CDS             complement(15117..16349)
                     /locus_tag="QBH27_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDQ7015538.1"
                     /translation="MYRDTRREYIVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQ
                     KRGVVSRHHGGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGK
                     VCVLGSFNVDIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKI
                     GRDQFNHFARNHIESIGIGSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSD
                     NDIIQLSHFIAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPF
                     VDIVTPNETEASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCA
                     HIPAFSAVAVDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPD
                     ISLVDARIQQQNVAIQTF"
BASE COUNT         4201 a         4360 c         4104 g         3804 t
ORIGIN      
        1 aagcgaggcg ctcaaaaaga gacaaggacc caaaccatga gccagcaagt cattattttc
       61 gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt gaaagaaaaa
      121 ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt cggtttcccc
      181 gtctcttcgc cgggtgattt tgaatcagtg cagaccatcg ctcgccaggt taaaaacagc
      241 cgtgtatgcg cgttagctcg ctgcgtggag aaagatatcg acgtggcagc cgaatccctg
      301 aaagtcgccg aagccttccg tattcatacc tttattgcca cttcgccaat gcacatcgcc
      361 accaagctgc gcagcacgct ggacgaagtg atcgaacgcg ctatctatat ggtgaaacgc
      421 gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccggacg caccccaatt
      481 gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac catcaacatt
      541 ccggacaccg tgggctacac catgccattt gagttcgccg gaatcatcag cggcctgtat
      601 gaacgcgtac ccaacatcga caaagccatt atttccgtac atacccacga cgatttgggc
      661 ctggcggtcg gcaactcact ggcggcggta catgccggag cacggcaggt agaaggcgca
      721 atgaacggga tcggcgagcg tgccggtaac tgttcccttg aagaagtcat catggcgatc
      781 aaagttcgta aggatattct caacgtccac accgccatca atcaccagga gatatggcgc
      841 accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa agccattgtt
      901 ggcagcggcg cattcgcaca ctcctccggt atccaccagg atggtgtact gaaaaaccgc
      961 gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca gctgaatctg
     1021 acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg atataaagaa
     1081 agtgaatata atttagacaa cctgtacgac gctttcctga agctggcgga caaaaaaggc
     1141 caggtgtttg attacgatct ggaggcgctg gccttcatcg ataagcagca agaagagccg
     1201 gagcatttcc gtctggatta cttcagcgtg cagtcaggtt ctaacgatat tgccactgcc
     1261 gccgtcaaac tggcctgtgg cgaagaagtt aaagcagaag ccgctaacgg caacggcccg
     1321 gttgatgccg tctaccaggc gataaaccgc atcactgact ataacgtcga actggtgaaa
     1381 tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga tattgtcgcc
     1441 aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt cgagtcctcc
     1501 gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt cgaaaaagag
     1561 ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa ccgtgtgatg tcgaagaatt
     1621 accatattgc cgtattgccg ggggacggta ttggtccgga agtgatgacc caggcgctga
     1681 aagtgctgga tgccgtgcgc aaccgatttg cgatgcgtat caccaccagc cattacgatg
     1741 taggcggcgc agccattgat aatcacgggc agccgctgcc gcctgcgacg gttgaaggtt
     1801 gtgagcaagc cgatgccgtg ctgtttggct cggtaggtgg tccgaagtgg gaacatttgc
     1861 cgccagacca gcaaccagaa cgcggcgcac tgctgccttt gcgtaagcac ttcaaattat
     1921 tcagcaatct gcgtccggca aaactgtatc aggggctgga agcattctgt ccgctgcgtg
     1981 ctgacattgc cgcaaacggc tttgacatcc tgtgtgtgcg cgaactgacc ggcggcatct
     2041 atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg tttgataccg
     2101 aggtgtacca ccgttttgag atcgagcgta ttgcccgcat cgcatttgaa tctgcccgca
     2161 agcgtcgcca caaagtcacc tcaatcgaca aagccaacgt gctgcaatcc tctattttat
     2221 ggcgggagat cgttaacgag atcgccacgg aatacccgga cgtcgaactg gcgcatatgt
     2281 acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac gtcttgctgt
     2341 gctccaacct gtttggcgac attctgtctg atgagtgcgc aatgatcact ggctcaatgg
     2401 ggatgttgcc ttccgccagc ctgaacgagc aaggttttgg tctgtatgaa ccggcgggcg
     2461 gctcggcacc cgatatcgca ggtaaaaata tcgccaaccc gattgcacaa attctgtcgc
     2521 tggcgctgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc attgaacgcg
     2581 ccattaaccg cgcattagaa gaaggcattc gcaccgggga tttagcccgt ggcgctgccg
     2641 ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa ggggtgtaat
     2701 catggctaag acgttatacg aaaaattatt cgacgctcac gtagtgtacg aagccgaaaa
     2761 cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct caccgcaggc
     2821 gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa ccttcgccac
     2881 catggatcac aacgtctcta cccagacgaa agacattaat gcctgcggtg aaatggcgcg
     2941 catccagatg caggagctga tcaaaaactg caaagaattt ggcgtcgagc tgtatgacct
     3001 gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggtg tgaccttgcc
     3061 ggggatgacc attgtctgcg gcgactcaca taccgccacc cacggcgcgt ttggcgcact
     3121 ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacgcaaa ccctgaaaca
     3181 gggccgggcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg gcattaccgc
     3241 aaaagatatc gtgctggcaa ttattggtaa aaccggcagc gcaggcggta ccgggcatgt
     3301 ggtggaattt tgcggcgaag caatccgtga tttaagcatg gaaggtcgta tgaccctgtg
     3361 caatatggca atcgaaatgg gcgcaaaagc cggtctggtt gcgccggacg aaaccacctt
     3421 taactatgtt aaaggccgtc tgcatgcgcc gaaaggtaaa gatttcgacg acgccgttgc
     3481 ctactggaaa accctgcaaa ccgacgaagg agcaactttc gataccgttg tcactctgca
     3541 agcagaagag atttcgccgc aggtcacctg gggcaccaat ccaggccagg tgatttctgt
     3601 gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgcg cgtcggcaga
     3661 aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgacagaag tggctatcga
     3721 caaagtgttt attggttcct gtactaactc gcgtattgaa gatttacgcg cggcagcgga
     3781 aatcgccaaa ggccggaaag tcgcgccagg cgtacaagcg ctggtggttc ccggctccgg
     3841 tccggttaaa gcccaggcgg aagcggaagg tctggacaaa atttttatcg aagctggttt
     3901 tgaatggcgc ttgccaggtt gctcaatgtg tctggcgatg aataacgacc gtctgaatcc
     3961 gggcgaacgt tgcgcctcca ccagcaaccg taactttgaa ggccgccagg ggcgcggcgg
     4021 gcgcacgcat ctggtcagcc cggcaatggc tgccgccgcg gctgtgaccg gacatttcgc
     4081 cgacattcgt aacattaaat aaggagcaca ccatggcaga gaaatttatc aaacacacag
     4141 gcctggtggt tccgttggat gccgccaatg tcgataccga tgcaatcatc ccgaaacagt
     4201 ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac tggcgttttc
     4261 tggatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg cagtatcagg
     4321 gcgcttccat tttgctggca cgagaaaact tcggctgcgg ttcctcgcgt gagcacgcgc
     4381 cgtgggcatt gaccgattac ggttttaagg tggtgattgc gccgagtttt gctgacatct
     4441 tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat gcagaagtgg
     4501 atgagctgtt tgcgctggtg aaagccaatc cggggattca ttttgatgta gacctggaag
     4561 cgcaagaggt gaaagcggga gagaaaactt atcgctttac catcgatgcc ttccgccgcc
     4621 actgcatgat gaacggtctg gacagcatcg gcctgactct gcagcacgac gatgctattg
     4681 cgtcttatga agagaagcaa ccggcgttta tgcgttagtt tgtacaccgt ccggcactca
     4741 atcgccaaaa gaaaaaagcc agcagataat cactgctggc gtggcgatta acaccaatac
     4801 tcagtcacac atgatgtccg caaatcagta gtcagaatga ctcccctccc attgcatcag
     4861 ttcttcaagc atggttaatc gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc
     4921 tgtcgcggta aaatagtgct gataaaactg acgcatgggg cacctccttg cttcatcgtt
     4981 aggaattatt gacttaatat agggaaaata aaattgctgt cttttgcgca ggagttcccc
     5041 ttttatgcca tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg
     5101 taaatctcag gagaccacgc tcaacgaact ggcagcgtta ctgagttgct cgcgtcgtca
     5161 tatgcgcacc ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt
     5221 cgggcgcggt aaacgctcgc gtctgacatt cctctatacc gggctggcgc ttcagcaaca
     5281 gcgagcggaa gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt tggttggcga
     5341 caaagcgact gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc gccaggggcg
     5401 gcacatcctg cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt
     5461 gcgccgttct gaaacccata tcgcccggca aatcttcagt tcgctaacgc gcataaatga
     5521 ggaaaatggg gaactggaag cagacatcgc acaccactgg cagcaaatat caccgcttca
     5581 ctggcgtttc tttttgcgtc caggagtcca ttttcaccat ggtcgtgaac tggaaatgga
     5641 cgatgtgatc gcctctttaa aacgaatcaa tacgcttccg ctctattcgc atattgctga
     5701 cattgtgtcg ccgacgccct ggacgctgga tatccatctc acgcaaccgg accgctggtt
     5761 gccgttactg ctggggcaag ttccggcaat gatcctgccg cgcgaatggg aaaccctcag
     5821 taactttgcc agccatccca tcggcaccgg tccgtatgcg gtgatccgca acagcaccaa
     5881 tcaactgaaa attcaggcat tcgatgactt cttcggttac cgggcattaa tcgacgaagt
     5941 taacgtctgg gttctaacgg aaattgccga cgagccagcc ggagggctga tgctgaaagg
     6001 gccgcagggc gaggaacaag agattgaaag ccgcctggag gaaggttgct attatttact
     6061 gttcgacaac cgcacccacc gcggggcgaa tcagcaagtc agggactggg taagctatgt
     6121 gctttctccg acgaatctgg tctatttcgc cgaggaacaa taccagcaac tctggttccc
     6181 ggcttatgga ctgctccccc gctggcacca tgcccgcccg acacattgcg aaaaacccgc
     6241 cgggctggaa catctcaccc tgacctttta tcaggatcat attgaacatc gagtgattgc
     6301 cgggatcatg cagcagattc tggcgagtca ccaggtcacg ctggaaatca aagagatcag
     6361 ctacgatcag tggcatgaag gagagatcga gagcgatatc tggcttaaca gcgccaactt
     6421 tacgctgccg ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc tactccaaca
     6481 ctgtattccg atcgactggc aagccgacgc cgcccgctgg cgcaatggcg aaatgaacct
     6541 ggcgaactgg tgccagcaac tggtcgccag caaagcaatg gtgccactta tccaccactg
     6601 gctgatcatt caggggcaac gcagtatgcg cggcctgcgc atgaacaccc tcggctggtt
     6661 cgattttaaa tcagcgtggt ttgcaccacc ggatccatga ttgctggttg ataacaaaat
     6721 cactacacta acgccgttct caacggggtg ccacgcagcc gcgtgcgctg agaaaatacc
     6781 cgtcgaacct gatccggata acgccggcga agggatttga ggctccttct caagtccttt
     6841 gccactcttt tgtgaggtgc aaagtgttaa aaaaatgtct tcccctgctg ttgctgtgca
     6901 cagcgcctgt tttcgctaaa cctgttctga ctgtttatac ctacgattcc tttgccgctg
     6961 actggggacc tggtccggta gtcaaaaaag cctttgaagc agactgtaat tgcgaactga
     7021 aactggtggc gctggaagat ggcgtttcgc tgctcaaccg tctacggatg gaaggcaaaa
     7081 acagcaaagc cgatgtggtg ctggggctgg ataacaacct gctggacgcc gccagcaaaa
     7141 ccgggttgtt tgccaaaagc ggcgtcgcga cagaggctgt aaacgttccc ggcggctgga
     7201 aaaatgacac ctttgtgccg tttgactatg gctatttcgc cttcgtctat gacaagaaca
     7261 aactgaaaaa tccgccgcaa agcctgaaag aactggttga gagcgatcaa aactggcggg
     7321 tgatttatga agatccgcgt accagtacac caggtctggg tctgttgcta tggatgcaaa
     7381 aagtctatgg cgataacgcc ccgcaagcct ggcagaaact ggcgaagaaa actgtcacgg
     7441 tcactaaagg ctggagcgaa gcctacggcc tgtttttaaa aggcgaaagc gatctggtac
     7501 tgagttacac cacctctccg gcttatcaca ttctcgaaga gaagaaagat aactacgccg
     7561 ccgcgaactt cagcgaaggt cactatctgc aggtggaagt tgccgcccgc accgctgcca
     7621 gcaagcagcc agagctggcg caaaaattcc tccagtttat ggtttctccg gcttttcaga
     7681 atgcgatccc aaccggcaac tggatgtatc cggtagcaaa cgtcactctg cctgcaggat
     7741 ttgaacaatt gaccaaacca gccaccacgc tagagttcac gcccgccgaa gtggcggcac
     7801 aacgtcaggc atggattagc gaatggcaac gcgccgtcag ccgttaattc ccggctggtt
     7861 aattccagga ataagcgccg ccacactggt ggtagcggtt gcgctggcgg cgtttctcgc
     7921 cctgtggtgg aacgcgccgc agggtgactg ggtggcagtc tggcaggaca gctatctgtg
     7981 gcatgtggtg cgcttctcct tctggcaggc gtttctctcg gcgctgctct ctgtcgtacc
     8041 cgcaatattc ctcgcccgcg cgctctatcg caggcgcttt cctgggcgac aggtgctgtt
     8101 gcgtctgtgc gcaatgacct tgatcctccc ggtgctggtc gccgttttcg gcattcttag
     8161 cgtctatggt cgccagggct ggctggcctc gctctggcaa tcacttggtc tggagtggac
     8221 cttttcgccc tacggcctgc aaggcatttt gctggcccat gtgtttttta atctgccgat
     8281 ggctagccgt ttattactcc aggcactgga aaacattccc ggcgaacaac gtcagcttgc
     8341 cgcacaactt gggatgcgcg gctggcactt cttccgcttc gtcgaatggc cgtggttacg
     8401 gcgacaaatc ccgccggttg ctgcgcttat ctttatgctc tgtttcgcca gcttcgccac
     8461 cgtgctatcg ctgggcggcg gtccgcaggc gaccactatc gagctggcaa tctatcaggc
     8521 gctgagttac gactacgatc ctgcccgcgc ggcaatgctg gcgctgatcc agatggtgtg
     8581 ctgcctcggg ctggtgctgt tgagtcagcg attgagtaag gccattgcgc ccggtaccac
     8641 actgctgcaa ggctggcgcg acccggacga tcgtctgcat agtcgcattt gcgacacggc
     8701 gttaattgtg ctggcgctgc tgctgttgct gccaccgtta ctggcggtga tcgtcgatgg
     8761 ggtaaatcgc cagttgccgg aagtgctggc acaaccggtg ctgtggcagg cgctgtggac
     8821 ctcgttgcgt attgcgctgg cggcaggcgt attgtgcgta gtgctgacca tgatgctgct
     8881 atggagcagt cgcgaacttc gggcacggca gaaaatgctg gcgggtcagg cgctggagat
     8941 gagcggcatg ttgatcctcg ccatgccggg gattgtactg gctacgggct tctttttact
     9001 gctcaacaac actatcggcc tgccacaatc tgctgacggc attgtgattt tcaccaatgc
     9061 gttaatggca atcccttatg cgctgaaagt actggaaaac ccgatgcgcg atatcaccgc
     9121 ccgctacagc atgttgtgtc agtcgctggg cattgaaggc tggtcacgct taaaagtggt
     9181 ggagctgcgc gctctgaaac gtccactggc gcaggcgctg gcctttgctt gtgtgctgtc
     9241 gattggtgat tttggcgtgg tggcattgtt cggtaacgat gatttccgta ccctgccgtt
     9301 ttatctctat cagcaaattg gctcctatcg cagccaggac ggcgcggtca ccgcgttaat
     9361 tctgctgctg ctctgttttc tgctgtttac cgtgattgaa aaaataccgg ggcgaaatgt
     9421 taaaactgac tgatatcacc tggctttacc accatttgcc gatgcgtttt agcttaacgg
     9481 tggaacgcgg cgagcaggtg gcgatcctcg ggccaagcgg cgcgggtaaa agcaccctgc
     9541 taaatttgat cgccggtttt ctgacgccag ccagcggttt gctgactctc gacggcgtag
     9601 atcacaccac aacgccaccg tcacgtcgtc cggtgtcaat gctgtttcag gagaacaacc
     9661 tgttcagcca cctgacggtc gcgcagaaca tcgggctggg gctaaatccg gggttgaaac
     9721 taaatgcggc gcagcagaag aaaatgcacg cgattgccca ccagatgggg attgataatt
     9781 taatggcgcg gttacctggc gagctttccg gcggtcagcg acagcgcgtg gcgttagcgc
     9841 gttgtctggt acgcgaacag cccattttat tgctcgatga accgttttcc gcactcgatc
     9901 cggcgctacg tcaggagatg ctgacgctgg tgagttcgag ctgccaacag caaaaaatga
     9961 cgctcttgat ggtgtcgcac agtgtggaag atgcggcgcg gatcgccacg cgctcggtgg
    10021 tggtcgccga cgggcgtatc gcctggcagg gtaagaccga tgagttgttg agcggtaagg
    10081 cgagtgcttc ggcgctgttg ggaattaaag gttaatatgc actgtcggat gcggcgtgaa
    10141 cgccttatcc gacctacagg tagtgcgttt tgtaggcctg ataagacgca gcaagcgtcg
    10201 catcaggcaa cccgcacaag gctatcctct aaaccccaac cactttacgc aaaatatcga
    10261 tatacaccgg catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag
    10321 aaatcaacgg cgtcagccac aacaaacgac cgcgggacaa ataatgactc aaacggtcag
    10381 tcgctttacc gctgcgccat aaccgccagc acagccagcc accaacccac aacaataccg
    10441 ccgtcaccag cagcaaccat ttaaactcac cgctctgcat cccggcagga atatcgatcg
    10501 ccgcacccgc cagaatcccc ggcaggaagt aaaacggtgg ccataacagg cagccgataa
    10561 tatttggcgt aataaatttc gccaccggca gatccagcat ccctgccacc attggcacca
    10621 gcggacgcgt cgggccgaca aaacgaccga ccagaatggt gaacatgctg tgctgatgca
    10681 gcgcatgttc ggttttatcg agtaatgctt tgttcttctt cagaaatgac cagcgatgca
    10741 acggcttttt aaaacgccag cccagccaga aggagatcca gtcgcccagc aggcaaccga
    10801 cgatccctgc cagccaggcg tgccagaaac ttaactcacc gctgccaatc aacgctccca
    10861 gccccgccat cagcaccgta ccgggcagaa tcaaaccgac cagcgccagc gactccagaa
    10921 aggccaccaa caccaccgcc atcaatgaat acacggtgga ttgggtaata aagtgttcca
    10981 gcaatgcttg cataatgtgc ccggttgatg aattcgtcgt ggattgtgca aagtggtggt
    11041 attcaggtca atctattctt tgtatgaata tattcctgca acgcgaatca tttagagtat
    11101 aaatttagca gtcacgcagg attttactta ggggaaggca gaccatccac aacttggatg
    11161 gtctgaaaac aacggattaa cggctaaatg aacggtagat attgcgtcct tgcaacgcga
    11221 caaccaaaaa gaaacagaca gccagtgcca tagataccgg gcgctcaaca aacgccagcc
    11281 attcgccatt agacttaatc attgaagata ataagttttt ttccaacatt ggcccaagaa
    11341 tcatccccag aataatgggc gaaaccgggt attccttacg ttgtaaccag tatccaagaa
    11401 cccccatgac caacatgaca acgacagaag cacccgagtt atcgatagca aatgcaccga
    11461 caatactgaa aatcagaata atcgggtaca gaatcgcttt atcaatccag atgatgcgtt
    11521 tgatgaaact gaccgcaatt gtggctaacg gtagtaatgc aatattggcg atcagaaaca
    11581 agataaatac ggcataaagt ttatctgcct ggaacagaaa taatgtcggc cctggattca
    11641 tatccttcat atagagcacg ccgatgataa ttgcagcggc agaatcgccc ggaataccga
    11701 aaactaatga agggatccaa ctccccgcca gactggcgtt gttactgctc gaggaatcaa
    11761 caattgcctc ctcagatcca tgcccatata actcctgctg gcgagagaat ttttttgaaa
    11821 tggcgtaact tatccacgca gcaatatccg ctccagcgcc gggtaatgct ccgataagcg
    11881 taccgataat actacttcgg ccaagattta attttcgtga ccaaatcacc tgccccattc
    11941 ccttgaataa attaatcccc gtttgcgttt gtggcacaac accacttacc tggctaattt
    12001 tgtcacgata atattcaatt gcgccggata tcgcgaacag accaatcatc gccgggataa
    12061 agttcacccc ttgtaacagg ctgatttgac caaacgtaaa acgtggaact ccggatattt
    12121 gatcataacc gatacacgcc agtaataatc ccaaacataa ggtcaccatg cctttcagtg
    12181 gttgattacc cacaaccaga gtcgcgcaag ttaagcctaa aagagaaagc catgtgtatt
    12241 cataagaact aaaattaagg gcaaatttcg ccagtacagg cgcagccaac atcaaaataa
    12301 gtgatccaat cacgccacca atgaccgaac tcaccagccc aagtcccaat acgcgattca
    12361 ccttcccttg cttgaccagt tcatttgaat cagcaacata agccgccgaa gcaggtgtcc
    12421 ctggtatacg caaaagtgcg ccggggatat ctccggcata aatggatgac gcaccaacgg
    12481 caatcattaa tgccagggcg ggaataggat caagaaagaa ggtaaaaggc accattagtg
    12541 caaccgccat cgttgcggtc aatccaggga tggcccccac aaataaccca aaggccgtag
    12601 cagcaataat ggccagaatg gcattcaaat taatatacgt tagtgcctga atcaaaatat
    12661 ccatattgca tccttaaaac aagattcctt caggaaaggc gaccaacaac actctggcaa
    12721 aaatgaacca gataataaga gatgccgata tcgaaacgat aacggaatgc aaaagtctga
    12781 cacctcgtat tttcattaaa ataaaaagca acaaaaatga gcaaatcaca aagccgagaa
    12841 tatctaacag taatacaaac agtagtacca ctgtaataac cagtaaaagt gctttcactt
    12901 catcaaaagt taaagaaact ttgttacggt gccggtgttt aatatctatc gctacatcaa
    12961 tagccgctaa aaacaacaac aatccactga tgatgcaagg cataaatcca gcgccatact
    13021 gcgatatgcc tccatattgc gttatgctcc agccaaaaat aaacatggca aaaatcagca
    13081 gaaataacgg cgttatcggt attatgcgca taatgctatc cctgtaattt ggcggcgata
    13141 tattcaccgc cctgtctgat taacctttga ttttggctat taattttccg tatctggcat
    13201 cttcatctgc cataaatgtc gtcatctctt tgccatacag cgggtaaact tcgaaaccct
    13261 gtttggtagc gaattcttgt aatttcccct gcgcaaacac tgcctgcata gcggcagtta
    13321 atttttgtga gacctcttca ggagtacccg ccggaaccac taaggcattc catgtcgcca
    13381 aatgatattt atagccagtt gcttgttgga atactggaag atctttataa agccctgctg
    13441 atttttctgc catggtcgct aaattacgca ccattttggc atcgaccata cttttcgctt
    13501 cgcctggtga tgacgttgta aaatctatac caccggaaac cagttccatc agtgcagaac
    13561 tggaaccttc agaaggcacc caggtcacgg cagaaggcgg aagccccata ctgtcgagca
    13621 tacctatcaa gttcagatgc cagccggaat ttaacccgct acccgatgct tttaacttac
    13681 ctggattcgc cttgatgtag tcggtcatct ggacgatatt ttcgaactgc gaatttgctg
    13741 cgacttgcac gcctcccaga ttcactgcca gccgtgtaat tggcgtgtag ttctgatacg
    13801 ttaacgccgt catcccctga tggtgcatca tggtgatttc taccgtcgca attcccaggg
    13861 tatagccatc cgctttagct ttcgcaatgg catcatggcc aaccacccca gcacctcccg
    13921 tgcgattcac cacgttgaca ttcactttca actctttttg cagcccttct gccaccaggc
    13981 gtgcaaccgt atcggtattt cctcccgcgc cccaggggac aatgatttgg attggttttt
    14041 ctggccatgc agccagcgcg gaacctgaaa ccagcagtga agcaacggca caactcatca
    14101 gggttcgttt catatttgta gactcctgtc ttaattattt tttaataaca gcaagtgctg
    14161 tcggatatta atatttgagt tgttgacgct ggaaatctgc ggcatcagca atttgtttta
    14221 gcaccacctc cagcccccaa tattttttca gcacatcgtc agccgttacc cggcaatgaa
    14281 cacagtcaaa tgttcctcgc tgctgatgaa atattttggc atttttcgga tagccaatat
    14341 tttcactgac ggcactcagc gataaaaacc aggcgagttg ttttgcctct tcagggtgtt
    14401 gttttccttc aagccaaagc cagtgataca agtcaggatg aaagttggca aagacgccac
    14461 aaaaaccttt cgaacctgca agcatcgcct cccaggcaat ggcagcatta gcattaacaa
    14521 tcgtcattgg cgtgtgctga gttaattgca cccgtcgcgt cactgtaggt agatcacaac
    14581 tgacgtcttt taagatttga aaacgcccgc tttgtgcgca gtaaagcagt tcctcatcgg
    14641 ttaataagcg gcgatacggg gccgggcatt catacagacc cagcgccata ctttcgggta
    14701 atgacatcaa tagcgcatca agcgtacgga ggaataccgt ggtaccttcg ttatgtggat
    14761 caagatgatt cgtcactaac accagcgcat caatgcccgt tgccgccata gcattgagct
    14821 cctgaatttg ctgttccaga tcatcagaaa tatgcccgga cgcaataacc ggtactcgtt
    14881 gttggctata ttcaaccaca aagctcgcca gctccacgcg ctcttctaaa gttaaatact
    14941 gcatttcact ggactgacac acggcaaata acgcgtcggc accgttttcg atataccagt
    15001 caattagccg tttaagaccc aggtaatcaa ttttattatc cgccgtaaaa ggcgttagca
    15061 ttaccggaat aatcccttca attgttttca tatttcatcc ttcgttcagt gaaacatcag
    15121 aaagtctgaa tggcaacatt ctgctgctga attctggcat ccaccagaga gatatcaggc
    15181 atattggctg ccccttcttt ttctactgcc agcgaagcga aagcgctggc ataccaggct
    15241 gctttaacaa gtgattctcc cttcgccagt gcggcggcga gcgcgccatt aaacgcatca
    15301 cccgcacctg ttgtatctac agcgactgcc gaaaatgccg gaatatgcgc acattgttgc
    15361 ccgtcataaa ttaatgcacc tcgttttccc atcgtaataa ttactgaaca ttgcccggca
    15421 ttataaatat gcttcgcagc cttcacagca tcagaaatat tgtcaattac catacctgac
    15481 atcgccgatg cttcggtttc atttggcgtc acaatatcaa caaaaggtaa taatgtggcg
    15541 acatcactgg accacggtgc aggatttaaa atcgttgtca cctgcatttt ttgagcacat
    15601 tttaatgcca gttgagtagc ggcaagatta ttttccatct gtacaatgaa tacatcggac
    15661 tcggctataa agtgagaaag ttgaataata tcgttatcac ttaattcatg atttgcccct
    15721 ggagacatag caatcatgtt ttcaccttca tcatttacat aaatgaccgc acaaccggtg
    15781 gtagatagtt cagactcata aaaagtaaat gaaccaatac caatggactc aatatgattg
    15841 cgggcaaaat gattaaactg atcgcgacca atcttacagg cgaagtgtat ttgcgcacct
    15901 gcccgatgag cggctagtgc ctgattcgcg cctttacccc ccggccccat ggtagtttcc
    15961 ctggccacta acgtttcgcc ttcattcggg aagcgataca ctttcgccac aatatcgacg
    16021 ttaaatgatc ctaacacaca aactttacct accattcggc gtcctttatc atcaagcgag
    16081 gttaaatagc caataccctg ctgttgttgc ttgataagca aactcatatc gacatactct
    16141 tgttgcgaaa gcgcattttg cattaaatgt tttttcaatg agacgcctcc atgatgacga
    16201 ctcactacac cacgcttttg caaccacgct aaatctgtcc gaatcgtctc tttggtaaca
    16261 gaaaaatagt tagctaaatc gctaacactg cactcatcct tgttgcgaag taaccggaca
    16321 atatattcac gacgggtatc ccgatacata agtcttcctt tttgatttta ttatcactaa
    16381 aaaagaaatt gttatcggga tttttatcaa gaaaaaaaca ggcaagcaaa aacaaacaaa
    16441 aatattattc aggcgaaacg attcaccta
//
LOCUS       DAOFSV010000098        16416 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.098, whole genome shotgun sequence.
ACCESSION   DAOFSV010000098 DAOFSV010000000
VERSION     DAOFSV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.098"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(74..994)
                     /gene="speB"
                     /locus_tag="QBH27_003259"
     CDS             complement(74..994)
                     /gene="speB"
                     /locus_tag="QBH27_003259"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDQ7015539.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1130..1861)
                     /locus_tag="QBH27_003260"
     CDS             complement(1130..1861)
                     /locus_tag="QBH27_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015540.1"
                     /translation="MKKWKLRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARIGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVHNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(2007..3983)
                     /gene="speA"
                     /locus_tag="QBH27_003261"
     CDS             complement(2007..3983)
                     /gene="speA"
                     /locus_tag="QBH27_003261"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDQ7015541.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(3992..4123)
                     /gene="yqgB"
                     /locus_tag="QBH27_003262"
     CDS             complement(3992..4123)
                     /gene="yqgB"
                     /locus_tag="QBH27_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDQ7015542.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            4259..4474
                     /locus_tag="QBH27_003263"
     CDS             4259..4474
                     /locus_tag="QBH27_003263"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015543.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            4778..5932
                     /gene="metK"
                     /locus_tag="QBH27_003264"
     CDS             4778..5932
                     /gene="metK"
                     /locus_tag="QBH27_003264"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDQ7015544.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            6368..7762
                     /gene="galP"
                     /locus_tag="QBH27_003265"
     CDS             6368..7762
                     /gene="galP"
                     /locus_tag="QBH27_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDQ7015545.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            7839..8336
                     /locus_tag="QBH27_003266"
     CDS             7839..8336
                     /locus_tag="QBH27_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDQ7015546.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLIAK"
     gene            8431..9138
                     /gene="endA"
                     /locus_tag="QBH27_003267"
     CDS             8431..9138
                     /gene="endA"
                     /locus_tag="QBH27_003267"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDQ7015547.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            9218..9949
                     /gene="rsmE"
                     /locus_tag="QBH27_003268"
     CDS             9218..9949
                     /gene="rsmE"
                     /locus_tag="QBH27_003268"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDQ7015548.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            9962..10912
                     /gene="gshB"
                     /locus_tag="QBH27_003269"
     CDS             9962..10912
                     /gene="gshB"
                     /locus_tag="QBH27_003269"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDQ7015549.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQVGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            11021..11584
                     /locus_tag="QBH27_003270"
     CDS             11021..11584
                     /locus_tag="QBH27_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDQ7015550.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            11584..12000
                     /gene="ruvX"
                     /locus_tag="QBH27_003271"
     CDS             11584..12000
                     /gene="ruvX"
                     /locus_tag="QBH27_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDQ7015551.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(12115..13095)
                     /locus_tag="QBH27_003272"
     CDS             complement(12115..13095)
                     /locus_tag="QBH27_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDQ7015552.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            13113..13817
                     /gene="yggS"
                     /locus_tag="QBH27_003273"
     CDS             13113..13817
                     /gene="yggS"
                     /locus_tag="QBH27_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDQ7015553.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            13835..14401
                     /gene="yggT"
                     /locus_tag="QBH27_003274"
     CDS             13835..14401
                     /gene="yggT"
                     /locus_tag="QBH27_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDQ7015554.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            14398..14688
                     /locus_tag="QBH27_003275"
     CDS             14398..14688
                     /locus_tag="QBH27_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDQ7015555.1"
                     /translation="MSAVTVNDGGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            14696..15289
                     /locus_tag="QBH27_003276"
     CDS             14696..15289
                     /locus_tag="QBH27_003276"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDQ7015556.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            15282..>16416
                     /gene="hemW"
                     /locus_tag="QBH27_003277"
     CDS             15282..>16416
                     /gene="hemW"
                     /locus_tag="QBH27_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDQ7015557.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3978 a         4361 c         4204 g         3873 t
ORIGIN      
        1 tgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa tgacacatca cccgtgcgcc
       61 tcttatctgg cgcttactcg ccctttttcg ccgcctgaat atacagcatc tccagcgcca
      121 gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta
      181 cgtccatccc aacgatgttg agatctttca ggccgcgtac cagtttaatg gcgcgatcgg
      241 aggtcaggcc gccaattacc ggcgtaccgg tgcctggtgc aaaagcagga tccaggcagt
      301 cgatatcaaa ggtcaggtaa accggcatat cacccacaat ctgtttcacc tgggcgataa
      361 tgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt
      421 tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt
      481 tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttcgca taggtatcgg
      541 tgtgggcgtc aaagtgtacc aacgccattt tgccgaaatg cttcgcatga gcgcgcagca
      601 gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttaccg gcagccagca
      661 gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg aaggcatata
      721 ccagatcacc gcagtccacg acgttcaggc gctcacgcat gtcgaaattc cacgggaagc
      781 ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtgacgac
      841 cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc cagtcagcat
      901 cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggcg ttggaaacca
      961 gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctttta caaagtcgat
     1021 ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt gcgaaacaca
     1081 agtttgtgct aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac
     1141 acggtggtgg cgcatagcca ttgttatctg ccggaaacgc ttgtccagag cacggcggca
     1201 gcgcgttttg cgccgggcct tgtaattcaa aggcggcaaa gccgattaca ccggtattat
     1261 gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg aactgaaaag
     1321 ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taacgaatct ccggcattca
     1381 caatataacc gttgttattc agcgaacctt gcttgccgtt cagcacatcc aggccatcaa
     1441 ccgtagcaac gatctcgtag ttggtgttac gactgtaatt acggatatat aattgataat
     1501 tcattccgat tctggcatgg acaatatagc gtggttcatt attggatgaa tcataaaccc
     1561 gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt tcgttaatac
     1621 gaatactata aacgcggtcg tagccggaag gaacattagt cgaataacta ataacgatca
     1681 tctctgccgg atcgcgccag gctcgttcag cagatacgcc ctgaaccgtt gagtgcacat
     1741 ccccacccca ggcggtgccg acattacccg atgcactgct attatactgc gcattactgg
     1801 aacaaccggc cagcagcacg gctaatgcta tcaacgcgct acgtaatttc cattttttca
     1861 taaccgttcc ctctgcattt aattaatgca gcgattgtag ggagattatt taataagaaa
     1921 agcagctaaa ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
     1981 tcgccccgcc agcggaataa tcttaattat tcatcttcaa gataggtata accgtacaaa
     2041 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
     2101 acctgatcgc ggaactgagt taacagcgtt ttcggatcga gctgcacata ttgcagcatg
     2161 tccgccacgg tatcgccttc gtcagacagt tctacctcta cgctaccatc agggaagacg
     2221 aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tgttgccaag gatctcctga
     2281 tatgcgccga ccataaagaa accgagcatc ggcggattct ccggatcgta ctccggcata
     2341 ggcattgtcg tggcaatacc gtcaccatca atatagtggt cgatagcacc gtctgagtca
     2401 caggtaatat ccaacagcac agcgcggcgt tccggcactt gatccagccc ttccagcggc
     2461 agaaccggga acaactggtc gatcccccac gcatccggca tcgactggaa cagcgagaag
     2521 ttgacgtaca ttttgtccgc catacgttcc tgcagctcgt cgataatcgg acgatgagca
     2581 cggttttgcg gatccagctg cttttgcact tcatggcaca tgctcaaata aagctgctca
     2641 gcccatgcgc gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatatcgtgc
     2701 agatccatct ggctgtcgtg taaccattca cgcagagaac ggcgagttcc cggttcgtgc
     2761 atttcctgcc aggtttccca catgctttgc agggcgcgcg gtgcgtcttc tgcaggcgcg
     2821 gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tcgacaccag cacggtgtga
     2881 tgcgcagtca ccgcacgacc cgattcggtg attaccgtcg gatgtggcag accattttct
     2941 tcacacgcat caccaatcgc ccagatgatg ttgttggcgt attcattgag gccgtagttc
     3001 accgaacagt cggactgcga acgggtgcct tcatagtcca cgcccagacc gccgccgacg
     3061 tcgaagcact gaatattgac gcccagcttg tgcagctcaa catagaaacg cgcggattca
     3121 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagc
     3181 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     3241 gtcgccgcca ggccgaactt cgatttttcc ccgccggagg actgccattt accggagccc
     3301 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     3361 tccagcacaa tggcaatttc tgacatcttc tcaatgacca gatagacctt gtggcccatc
     3421 ttctcgccaa ttaatgccag acggatatat tcgcggtcct tataaccgtt gcagacgatg
     3481 acgctacggg tcatgccagc atgtgccagc accgccatca actcagcttt ggaaccggct
     3541 tccaggccca gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg gtgctggtta
     3601 actttgatcg gataaacgag gaagtaatcg ccgttatagc cgtaggattc ccgcgcacgt
     3661 ttgaacgcgg cgttaataga acgcaaacgg tgctgcagga tctgcgggaa acagaacagt
     3721 gcaggcagac gctggccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     3781 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     3841 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     3901 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     3961 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     4021 taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     4081 gtgaatgctg acgctccaac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     4141 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     4201 cgcccttgtt cagtcctcag tagctgtaac cagctcttga atcctgagaa gcgccgagat
     4261 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac
     4321 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     4381 gccgcctgtt aagacgaacc cacaagccaa aactctgttt tcaacccggc tggaagtggc
     4441 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     4501 aatggcagaa aattgcaaaa gataaatacg cagaatgcca gcattgtcag gaaaaatttc
     4561 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatt gggtgaagaa
     4621 ctgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     4681 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     4741 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     4801 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     4861 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     4921 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     4981 cacccgtaac accgttcgcg aaattggcta tgtacattcc gacatgggct ttgacgctaa
     5041 ctcctgtgcg gttctgagcg ccatcggcaa acagtctcct gacatcaacc agggcgttga
     5101 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     5161 caatgaaacc gacgtgctga tgccagcgcc tatcacctat gcacaccgtc tggtacagcg
     5221 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     5281 ggtgactttc cagtatgacg acggcaaaat cgttggtatc gatgctgtcg tgctttccac
     5341 tcagcactct gaagaaatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     5401 caagccgatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     5461 cggtcgtttt gttatcggtg gcccgatggg tgactgcggt ctgactggtc gtaaaatcat
     5521 cgttgatacc tacggcggca tggcacgtca cggtggtggt gcattctctg gtaaagatcc
     5581 atcaaaagtg gaccgttccg cagcgtacgc agcacgttat gtcgcgaaaa acatcgttgc
     5641 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     5701 gacctctatc atggtggaaa ctttcggtac cgagaaagtg ccttctgaac aactgactct
     5761 gctggtacgt gagttctttg acctgcgtcc atacggtctg attcagatgc tggatctgct
     5821 gcacccaatc tacaaagaaa ccgcagcata cggtcacttt ggtcgcgaac atttcccgtg
     5881 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatttttctt
     5941 cacctgcttt taaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     6001 aggcgttcat gccgcatccg gcaaaaagct gcccgcacaa taacatcatt cttcctgaac
     6061 acgttttacc gcaaattatc atcgctactg aaaccgatta caccaaccac gccagccaaa
     6121 tatttgtaat attttcatat tattattcag ctttcacagt tgttacattt cttttcagta
     6181 aagtcttaat tgcagataac agcgtttaat ctatgatgat ataactcaat tattttcatg
     6241 tacttaaatc ataactaaga taaatgttag tgtaagcgat tacactgatg tgatttgctt
     6301 cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa ccatattgga
     6361 gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt
     6421 ctgcttcctt gctgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg
     6481 cgcactgccg tttattgcag atgaattcca gattacttcg cacacgcaag aatgggtcgt
     6541 aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc tctcctttaa
     6601 actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg gttcactgtt
     6661 ctctgcggct gcgccaaacg ttgaagtact gattctttcc cgcgttctgc tggggctggc
     6721 ggtgggtgtg gcttcttata ccgcaccact gtacctgtca gaaattgcgc cggaaaaaat
     6781 tcgcggcagt atgatctcga tgtatcagtt gatgatcact attgggatcc tcggtgctta
     6841 tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat
     6901 catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc cacgttggtt
     6961 tgctgccaaa cgccgttttg ttgatgccga acgcgtgctg ctgcgcctgc gtgacaccag
     7021 cgcggaagcg aaacgtgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg
     7081 ctgggcgctg tttaaagaga acagcaactt ccgccgcgcg gtgttccttg gcgtactgtt
     7141 gcaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt
     7201 cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacggtga ttgtcggcct
     7261 gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc
     7321 aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat
     7381 gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt
     7441 tattgttggc tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca
     7501 gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa
     7561 catgatcgtt ggcgcaacct tcctgaccat gctcaacacg ctgggtaacg ccaacacctt
     7621 ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattgtggc tggtaccgga
     7681 aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg
     7741 cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccacct
     7801 cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta
     7861 tcgccatcca acaggccgtt atgcgtcgcc tgcgggaaaa actcgcccag gccaacctga
     7921 agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg
     7981 gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca
     8041 gtgaagcctt tattgaagaa gtggtgccgc acgaactggc acatttgctg gtatggaaac
     8101 atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg
     8161 gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc
     8221 cctaccgctg caagtgccag gagcatcagc ttaccgtacg ccgccataat cgcgtagttc
     8281 gtggcgaggc cgtctatcgc tgtgttcact gcggtgaaca gctgattgca aagtaaccac
     8341 ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg
     8401 ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg
     8461 gtactgagcg cagcattttc cggcccggcg ttggccgaag gtatcaatag tttttctcag
     8521 gcgaaagccg cggcggtaaa agtccacgct gacgcgcccg gtacgtttta ttgcggatgt
     8581 aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg
     8641 cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa
     8701 ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc taaagatccg
     8761 gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg cgaggtgaat
     8821 ggcgatcgcg gcaactttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa
     8881 tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt
     8941 gccattgcgc gcacctactt ctatatgcgt gaccaataca acctgacact ctctcgccag
     9001 caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg ggagtgcgag
     9061 cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc
     9121 caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc taccccacgc
     9181 gaacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca
     9241 tcctgaacca ctgaccagcc attctcatat cgcgctttgc gaagatgctg ccaaccatat
     9301 cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg gcagcaacca
     9361 ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtgttgga
     9421 aggacagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg
     9481 tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa gcctcattac
     9541 gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct
     9601 ccagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc
     9661 ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca gagcaggatg aaggactgaa
     9721 actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgccgttac cggttgaacg
     9781 cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc
     9841 ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc
     9901 gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat
     9961 aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc
    10021 cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat
    10081 gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa
    10141 gcagaactac gaagagtggt tttcgttcgt cggtgaacag gatctgccgc tggccgatct
    10201 cgacgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta
    10261 tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg
    10321 cgactgtaac gagaaactgt ttactgcctg gttctctgac ttaacgccag aaacgctggt
    10381 tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa
    10441 gccactggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct
    10501 cggcgtgatt gccgaaaccc tgactgagca tggcactcgc tactgcatgg cgcaaaatta
    10561 cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtagatggcg agccggtacc
    10621 gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg
    10681 tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc aggtcgggcc
    10741 gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg accgtctgac
    10801 tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc
    10861 gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt
    10921 agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg
    10981 cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt
    11041 tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg
    11101 cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa
    11161 aattgaaggg attctggaaa agctgaagat cacgccggag ccgcgtgatg aatcaatccg
    11221 tctggataaa ccggttatgc tcggcggccc tctggctgaa gatcgcgggt ttattttgca
    11281 tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacgg tgatgaccac
    11341 ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc
    11401 tctgggctat gcctcctggg agaaaggtca actggaacaa gaaattctcg ataacgcgtg
    11461 gctaacggcc ccggcggatc tgaatattct gttcaaaaca ccgattgccg accgctggcg
    11521 cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg cagggcacgc
    11581 ctgatgagtg gaaccttact cgccttcgac ttcggtacca aaagcattgg tgtagcggtc
    11641 ggccaacgca ttaccggcac cgctcgccca ttgcctgcaa ttaaagcaca ggacggtacg
    11701 ccggactgga acctcatcga acgtttactg aaagagtggc agccggacga aatcatcgtc
    11761 ggtttgccgc tgaatatgga cggcaccgag caaccattga ctgccagagc gcgtaaattt
    11821 gccaaccgta ttcatggccg tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc
    11881 accgtggagg cacgttccgg tctgtttgaa cagggcggct atcgggcgct caacaaaggc
    11941 aaagttgact ctgcctctgc ggttatcatt ctcgaaagtt atttcgagca gggatattaa
    12001 ggcgatttac gtcatcgctc cttattgccg gatgcggcgt gaatgactac gtttgtaggc
    12061 ctgataagcg tagcgcatca ggcatattta ctaaaataat caatcacttt agcctcacaa
    12121 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    12181 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    12241 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    12301 cagcttttgt gacaacactg cccgtaaact ccccgccagt tgattacgta cgggatcttt
    12361 ttcctgcgcc ggaaatgaat ccaccagccg ctcaactgcc tgcgccgcac cacgcgtatg
    12421 taatgttgcc agcaccaaat gcccggtttc tgccgccgtc agtgccagac ggattgtctc
    12481 gctgtcacgc agctctccga gcaatatcac atcgggatct tcccgcaatg cggcacgcaa
    12541 tcccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    12601 gctggcatag agatattcaa caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    12661 attgagatag ccaaccatcg ccgccagcgt ggtagatttg ccgctccccg tcgcccccgt
    12721 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    12781 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    12841 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacag caaaatccaa
    12901 ctgaccattc tccagcaata ttgtccgctg atcgtcatcc agccactccc gcagtagctc
    12961 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattcgc ccgcgaatac gccatcgtgc
    13021 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    13081 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    13141 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    13201 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    13261 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    13321 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    13381 gccgcctggt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    13441 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    13501 atattagtga cgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    13561 ctgcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcacctg
    13621 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    13681 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    13741 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    13801 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    13861 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    13921 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtaacgcagc caattatcgg
    13981 gccactgcgc cgcgttattc cggcaatggg gcccattgac agcgcctcac tgctggttgc
    14041 gtacattctc agtttcatca aagccatcgt gctgtttaaa gtggtgacct tcctgccaat
    14101 catctggatt gccggtttac tgattctgct gaaaaccatc ggcctgctga ttttctgggt
    14161 cctgctggtg atggcgatta tgagctgggt aagccagggg cgtagcccga ttgaatacgt
    14221 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcctgctac cggcaatggg
    14281 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    14341 cgcagaagtc ttgcaggcga ccggaaatat gctgctgccg gggctgtgga tggcgttatg
    14401 agtgccgtaa cagtgaatga tggcggtctg gttttacggc tctatattca gccgaaagcc
    14461 agccgtgatt ctattgtcgg tttacatggc gacgaggtaa aagtcgccat taccgcgccg
    14521 ccggttgacg gccaggccaa cagccatctg gtgaagtttc tcggtaagca attccgggtt
    14581 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    14641 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    14701 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    14761 cgacttcggt cttgatatcg tggcccaaac agacctcggt gtagattccg ctgaagaaac
    14821 tggcctgacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    14881 tttaccggca attgccgacg actccggtct ggcggtagat gtacttggcg gtgcaccggg
    14941 gatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    15001 gctggaaaca atgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    15061 ggtatatctg cgtcacgcgg aagatcccac tccgctggtg tgccacggta gctggccggg
    15121 cgtgattact cgtgaaccag cgggcactgg tggctttggt tatgatccaa tcttctttgt
    15181 tccttccgaa gggaaaaccg ctgccgaact gacccgcgaa gaaaagagcg ccatttccca
    15241 ccgtggtcag gcattgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    15301 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    15361 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg cttaacgatc
    15421 tggacaacga tgtggcttac gctcagggcc gtgaagtgca gacaatcttt attggcggtg
    15481 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctggacggc gtgcgtgcgc
    15541 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    15601 cggatcgctt tgtcgattat cagcgtgctg gtgtgaaccg catctctatt ggtgtgcaga
    15661 gttttagcga agaaaagctg aaacgactcg ggcgtattca tggcccgcaa gaagcgaaac
    15721 gagcggcaaa gctggcgagc ggtttagggt tacgtagttt taaccttgat ttgatgcatg
    15781 ggctgccgga tcaatcactg gaagaggcgc taggcgatct gcgccaggct attgaactga
    15841 atccgccaca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    15901 gccctcctgt actgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    15961 tattaaccgc agcgggctat cagcagtatg aaacatccgc ttacgccaaa cccggttatc
    16021 agtgccagca caatctcaac tactggcgat ttggcgacta catcggtatt ggctgcggcg
    16081 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccaccaaa acgcgtcatc
    16141 cgcgtggttt tatgcaggga agatatctgg aaagccagcg tgatgtcgaa gctgcagata
    16201 aaccgtttga gttctttatg aatcgcttcc gtctgctgga agccgcgccg cgcgtggagt
    16261 ttatccaata tactggcctt tctgaagaag ttattcgctc tcagttagac gaggccatcg
    16321 ctcaaggtta tctcacagaa tgtgcggatt actggcagat aaccgagcat ggtaaattat
    16381 tcttaaactc acttcttgaa ttattcctcg ctgagt
//
LOCUS       DAOFSV010000099        16219 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.099, whole genome shotgun sequence.
ACCESSION   DAOFSV010000099 DAOFSV010000000
VERSION     DAOFSV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.099"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     misc_feature    <1..88
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            110..1270
                     /gene="pheA"
                     /locus_tag="QBH27_003278"
     CDS             110..1270
                     /gene="pheA"
                     /locus_tag="QBH27_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDQ7015558.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELVVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1313..2434)
                     /gene="tyrA"
                     /locus_tag="QBH27_003279"
     CDS             complement(1313..2434)
                     /gene="tyrA"
                     /locus_tag="QBH27_003279"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDQ7015559.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLILSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2445..3515)
                     /gene="aroF"
                     /locus_tag="QBH27_003280"
     CDS             complement(2445..3515)
                     /gene="aroF"
                     /locus_tag="QBH27_003280"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDQ7015560.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            3725..4090
                     /locus_tag="QBH27_003281"
     CDS             3725..4090
                     /locus_tag="QBH27_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDQ7015561.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            4237..4755
                     /locus_tag="QBH27_003282"
     CDS             4237..4755
                     /locus_tag="QBH27_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDQ7015562.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            4745..5971
                     /gene="dgcN"
                     /locus_tag="QBH27_003283"
     CDS             4745..5971
                     /gene="dgcN"
                     /locus_tag="QBH27_003283"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDQ7015563.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNSETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            5987..6469
                     /locus_tag="QBH27_003284"
     CDS             5987..6469
                     /locus_tag="QBH27_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDQ7015564.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLITQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(6546..6893)
                     /gene="rplS"
                     /locus_tag="QBH27_003285"
     CDS             complement(6546..6893)
                     /gene="rplS"
                     /locus_tag="QBH27_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDQ7015565.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(6935..7702)
                     /gene="trmD"
                     /locus_tag="QBH27_003286"
     CDS             complement(6935..7702)
                     /gene="trmD"
                     /locus_tag="QBH27_003286"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDQ7015566.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTYD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(7733..8281)
                     /gene="rimM"
                     /locus_tag="QBH27_003287"
     CDS             complement(7733..8281)
                     /gene="rimM"
                     /locus_tag="QBH27_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDQ7015567.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8300..8548)
                     /gene="rpsP"
                     /locus_tag="QBH27_003288"
     CDS             complement(8300..8548)
                     /gene="rpsP"
                     /locus_tag="QBH27_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDQ7015568.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(8685..10046)
                     /gene="ffh"
                     /locus_tag="QBH27_003289"
     CDS             complement(8685..10046)
                     /gene="ffh"
                     /locus_tag="QBH27_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDQ7015569.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10213..11004
                     /locus_tag="QBH27_003290"
     CDS             10213..11004
                     /locus_tag="QBH27_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDQ7015570.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            11025..12311
                     /locus_tag="QBH27_003291"
     CDS             11025..12311
                     /locus_tag="QBH27_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDQ7015571.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12365..12958)
                     /gene="grpE"
                     /locus_tag="QBH27_003292"
     CDS             complement(12365..12958)
                     /gene="grpE"
                     /locus_tag="QBH27_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDQ7015572.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13081..13959
                     /gene="nadK"
                     /locus_tag="QBH27_003293"
     CDS             13081..13959
                     /gene="nadK"
                     /locus_tag="QBH27_003293"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDQ7015573.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14045..15706
                     /gene="recN"
                     /locus_tag="QBH27_003294"
     CDS             14045..15706
                     /gene="recN"
                     /locus_tag="QBH27_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDQ7015574.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGYGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            15855..16196
                     /gene="bamE"
                     /locus_tag="QBH27_003295"
     CDS             15855..16196
                     /gene="bamE"
                     /locus_tag="QBH27_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDQ7015575.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
BASE COUNT         4099 a         4264 c         3813 g         4043 t
ORIGIN      
        1 ccttcccctg aatgggaggc gtttcgtcgt gtgaaacaga atgcgaagac gaacaataag
       61 gcctcccaaa tcggggggcc ttttttattg ataacaaaaa ggcaacacta tgacatcgga
      121 aaacccgtta ctggcgctgc gagaaaaaat cagcgcgctg gatgaaaaat tattagcatt
      181 actggcagag cgacgcgaac tggtcgtcga ggtgggaaaa gccaaactgc tttcgcatcg
      241 cccggtacgt gatattgatc gtgaacgcga tttgctggaa agattaatta cgctcggtaa
      301 agcgcaccat ctggacgccc attacattac tcgcctgttc cagctcatca ttgaagattc
      361 cgtattaact cagcaggctt tactccagca acatctcaat aaaattaatc cgcactcagc
      421 acgcatcgct tttctcggcc ccaaaggttc ttattcccat cttgcggcgc gccagtatgc
      481 tgcccgtcac tttgagcaat tcattgaaag tggctgcgcc aaatttgccg atatttttaa
      541 tcaggtggaa accggccagg ccgactatgc cgtcgtaccg attgaaaata ccagctccgg
      601 tgccataaac gacgtttacg atctgctgca acataccagt ttgtcgattg ttggcgagat
      661 gacgttaact atcgatcatt gtttgttggt ctccggcact actgatttat ccaccatcaa
      721 tacggtctac agccatccgc agccgttcca gcaatgcagc aaattcctta atcgttatcc
      781 gcactggaag attgaatata ccgaaagtac gtctgcggca atggaaaagg ttgcacaggc
      841 aaaatcaccg catgttgctg cgttgggaag cgaagctggc ggcactttgt acggtttgca
      901 ggtactggag cgtattgaag cgaatcagcg acaaaacttc acccgatttg tggtgttggc
      961 acgtaaagcc attaacgttt ctgaccaggt tccggcgaaa acgacgttgt taatggcgac
     1021 cggacaacaa gctggtgcac tggttgaagc gttgctggta ctgcgcaacc acagtctaat
     1081 tatgacccgt ctggaatcac gtccgattca cggtaatccg tgggaagaga tgttttatct
     1141 ggatattcag gccaatcttg aatcagcgga aatgcaaaaa gcattgaaag agttagggga
     1201 aatcacccgt tcgatgaagg tattgggctg ttacccaagt gagaacgtag tgcctgttga
     1261 tccaacctga tgaaaaagtg ccggatgatg tgaatcatcc ggcactggat tattactggc
     1321 gattgtcatt cgcctgacgc aataacacgc ggctttcact ctgaaaacgc tgtgcgtaat
     1381 cgccgaacca gtgctccacc ttgcggaaac tgtcaataaa cgcctgctta tcgccctgct
     1441 ccagcaactc aatcgcctcg ccgaaacgct tatagtaacg tttgattaac gccagattac
     1501 gctctgacga cataataatg tcggcataaa gctgcggatc ctgagcaaac agtcgcccga
     1561 ccatcgccag ctcaaggcgg taaatcggcg aagagagcgc cagtagttgc tcaagctgaa
     1621 cattttcttc tgccaggtgc agcccataag caaaagtagc aaagtggcgc agagcctgaa
     1681 taaacgccat attctgatcg tgctcgacag cgctaatacg atgcaaccga gcgccccaga
     1741 cctgaatttg ctccagaaac cattggtatg cttccggctt acgtccatca caccagacca
     1801 caacttgctt tgccaggcta ccgctgtccg ggccgaacat cgggtgtaac cccagcaccg
     1861 ggccatcgtg cgccgccagc atggcctgta atggtccatt tttcactgat gccagatcaa
     1921 ccagaataca atctttcggt aaaggcggta atttgccgat aacttgctca gtaacgtgga
     1981 tgggcacact aacaatcacc attccggcat cggcaacaat atcagccgct cgatcccagt
     2041 catgttgctc cagaatccgc acctgataac ccgagagtat cagcatcttc tcgaacagac
     2101 gtcccatctg accgccacca ccgacgataa ccaccgggcg cagtgacgga caaagcgttt
     2161 taaatccttt gtcgttttca ctggagtaag attcacgcat cacccgacgc aaaacatcct
     2221 caatcagatc tggcggtaca cccagagctt ccgcctccgc acgacgcgag gccaacatag
     2281 atgcctcgcg ctccggaaca taaataggca gtccaaatcg gcttttcacc tcgcccactt
     2341 cagcaaccaa ttccagacgc ttcgctaata aattcagcag cgctttatcg acttcatcaa
     2401 tttgatcgcg taatgcggtc aactcagcaa ccataataaa cctcttaagc cacgcgagcc
     2461 gtcagctgcc cgttcagatc ctgatgaatt tcacgcagca aggcatcggt catttcccag
     2521 ctaatgcagg catcggttac ggatacaccg tatttcattt cactgcgcgg ttgctcggaa
     2581 gactgattgc cctcgtggat attactttcg atcatcagac caataattga gcgattgcca
     2641 tctttgattt gagcaaccac ggattctgcc accgcaggct gacggcgata atctttattg
     2701 gaattaccgt ggctgcaatc taccatcaga gacgggcgca gtcccgcctg ttccatctct
     2761 ttttcacatt gcgcaacatc cgcagggcta tagtttggcg ctttaccacc gcgcaggatc
     2821 acatgcccgt ccggattccc ctgagtttgt agcaacgcaa cctgccctgc ctggttaatg
     2881 ccaacaaaac ggtgcggctg ggcggcggcg cgcatagcgt taattgccgt tgccagactg
     2941 ccatcggtgc cgtttttaaa accaaccggc atggaaagcc cggaggccat ttcacggtga
     3001 gtttgcgatt ccgttgtacg agcaccaatt gctgaccagc taaacagatc gcccaggtat
     3061 tgcgggctat tcggatctaa cgcttccgtc gccagtggca gtcccatatt caccagctca
     3121 agcagcaatt tacgcgcgat ctgcagcccg gcttctacat caaaagagcc atccatatgg
     3181 ggatcgttaa ttaacccttt ccagccgaca gtggtacggg gtttttcaaa atagacgcgc
     3241 attaccagat agaggctatc gctgacctct gcggcaaggg ctttaaatcg acgagcatat
     3301 tccagagcag tttccggatc atgaatggaa caaggaccac atactaccag aagacgagga
     3361 tcgcgcccgg cgataatatc tgaaatggtt ttacgcgagt cagcaatctg ggcttcttgt
     3421 tgcaggctta atggaaaagc ggccttcagt tgttccggag tcattaaaac ctgttcgtcg
     3481 gtaatatgta cgttattcag cgcgtctttt tgcatgatgg cgatcctgtt tatgctcgtt
     3541 tgcgatagtt gatcctcagc gaggatgacg taacgataac acataaagta aagttttcaa
     3601 tccatatttc gtacacattt ctttacgtaa gcaatttatt cgcgcttttt acccttattt
     3661 ctgtatatat aaatttacac catctttaaa aacaatccgc tatgctttga aaaaggagaa
     3721 agaaatgatg aaaaaattta tcgccccctt gttggcttta ctggttagcg gatgtcagat
     3781 tgatccttat actcacgcgc caaccttgac cagcaccgac tggtatgatg ttggtatgga
     3841 agatgcgata tcgggcagcg ccattaaaga tgacgatgca tttagcgatt cacaggcgga
     3901 tcgcggtcta taccttaaag gatatgccga agggcaaaag aaaacttgcc agaccgattt
     3961 tacttatgcc cgggggcttt ctggtaaaag ctttcctgcc agctgcaata atgttgaaaa
     4021 tgccagccaa ctgcatgaag tatggcgaaa aggggctgat gaaaatgcca gcacaatacg
     4081 attaaattaa ttacccccaa aataaattaa gcatgagatt ttactcatta tgttttattt
     4141 ctcacattga tgacgaccgc atgaaaggat gatattttaa tgagcattct gaaaaaatat
     4201 cgatgcattt cgagcgaaga tggtgaggat ccctgaatgc gtttttctca ccgactgttc
     4261 ttactgctga ttctcctgct gacgggagca ccaatactcg ctcaggaacc ttctgacgtt
     4321 gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg gcctgggcta
     4381 tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc cttacaagaa
     4441 aatgctgcta catctttacc ttatctgcct gtcatcattc atactcaaca agaggcgata
     4501 atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca gatggaatta
     4561 accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac cgagtgcatt
     4621 attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc cgtaaacctg
     4681 gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct cgcacggaag
     4741 aaaaatgatg gataacgata attctcttca taagcgcccc acgtttaaaa gagcattacg
     4801 caacatcagc atgaccagca tatttatcac tatgacgctg atctggctgc tgctttccgt
     4861 gacgtcagtg ctaaccctga aacaatacgc gcaaaaaaac ctggcactga cagcagcaac
     4921 aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag caactgaaac
     4981 actggcagcg ctgggccagc aaggccaatt ttcaactgca gaagtgcgtg ataagcagca
     5041 aaatattctg gcatcctggc attacaccca taaggaacca ggcgatactt tcagcaattt
     5101 cataagccac tggctcttcc ctgcccccat cattcagccg attcgtcaca atagtgaaac
     5161 cattggcgaa gtacgcttaa ctgctcgcga cagttcaatc agccatttta tctggttttc
     5221 gctcgctgtc ctgaccggtt gtattctgct ggcatcaggc atcgcaatta ccctcacccg
     5281 ccatttgcac aatggcctgg tggaagcgct gaaaaatatc accgatgtcg tacatgatgt
     5341 gcgttctaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg
     5401 cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca
     5461 ggccaaaaat gcgcagcttc tacgtaccgc gctacatgac ccattaaccg ggctggctaa
     5521 ccgcgcagcg tttcgtagcg gcatcaacac gttgatgaac aattccgatg cccgaaaaac
     5581 gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata cctggggtca
     5641 tgcgacgggc gatagagtct tgattgaaat agcaaaacgg ttagctgaat ttggcgggct
     5701 gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct atgatgtaca
     5761 gtcggaatct gaagtgcagc agatatgctc agcactgaca caaattttta atctcccgtt
     5821 tgatcttcat aatggccatc aaactaccat gacattaagc attggttacg cgatgaccat
     5881 tgagcacgcc tctgcggaaa atttacaaga gcttgccgat cacaatatgt atcaggccaa
     5941 acaccagcgt gccgaaaaac tggtgagata acaaggatat atcgatatga taaagcacct
     6001 gctagccccc ctgattttca cctcgctaat acttactggc tgccagtccc ctcagggaaa
     6061 gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat ccgccggcga
     6121 ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca aattgctccc
     6181 ggaaagccag caacaaatcc aaaccatggc agccaaattg gcctcgacag ggctaacaca
     6241 tgcccgtatg gatggacaca ccgacaacta tggtgaagac agttacaacg aagtcttatc
     6301 attaaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa ttccgcgcag
     6361 caatctcatc acacagggtt taggaaaaaa atatcccata gccagtaaca agaccgccca
     6421 gggccgcgcc gaaaaccgcc gcgtcgcagt ggtgattaca accccttaaa acacgagcaa
     6481 acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt cgcttaagcg
     6541 aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc acgcaggtag
     6601 tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc aactaccgga
     6661 gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg aacagtgaat
     6721 gcagagtgca gaccgcggtt acgaattgcg ataaccacgc cctcgaatgc ctgcagacgt
     6781 tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg acggaaggaa
     6841 ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct cataatttaa
     6901 tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca tgtttatgtt
     6961 gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct tcagtcagag
     7021 ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac tgtttcaaac
     7081 gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact tccatccctt
     7141 ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca aaggagtctt
     7201 ccgttgccga ggcctcatgt cccagtactc ccggaataaa ccgggaaacg gagtcaatca
     7261 gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac cattcttcat
     7321 caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac accagaatca
     7381 atttttgatt cgttgccagc tcgctgacgc ccgcttgatc aagcttgcgt ccctgtggtg
     7441 acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga atggcgtccc
     7501 gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga tcgtccacgg
     7561 tacggtgccg atcatacgtg aagtcgcgag gactccagct ctggatgctc agcaggccat
     7621 ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt tcaggaaaca
     7681 ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg gtttaaaaac
     7741 caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg ataacctgcc
     7801 catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg tttgccttaa
     7861 tgacgagaac gtcattagat ccggtttcca tcatatctac gactttaccg agatcgtagc
     7921 cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtca ccctcttcaa
     7981 gctgaggcaa ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat
     8041 cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc ttccagcttt
     8101 ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga tagtcaaaaa
     8161 tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata ccgtaagacg
     8221 aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt tgtttgctca
     8281 tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc gcagcaacgc
     8341 gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga tccaggcgag
     8401 tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg atgaagcgac
     8461 cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc tttttagcgc
     8521 cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa acaaccggac
     8581 cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac tgatttctgg
     8641 agaaaatgca atcagcagtt aataactctg tcgcataagg tcatttagcg accaggaaat
     8701 cccggcggca tcatcccttt catgcttctc atcatcttcg ccatcccgcc cttcttcatt
     8761 ttcttcatca tgcgctgcat gtcgtcgaac tgtttcagaa gacggttaac gtcctgcacc
     8821 tgcataccgc aaccggcagc gatacggcgc ttacgcgaac ctttgatgat ttctggctta
     8881 gcgcgctctt tcatcgtcat cgagttgatg atggcttcca tacgcaccag cactttatcg
     8941 tccatctgcg acttgacgtt atccgggatc tgccccatgc ccggcagctt gcccatcagg
     9001 ctagccatgc cgcccatatt tttcatctgg cgcagctgct caagaaagtc gttgagatcg
     9061 aagccgtcgc cttttttcag cttgctggct aatttctcag cttgcgcgcg gtcaactttg
     9121 ctttcgatat cttcgatcag tgacagtacg tcgcccatgc cgagaatacg ggaggcaata
     9181 cggtccggat ggaatggctc cagcgcctcg gttttctcgc caacaccgag gaacttgatc
     9241 ggtttgccag tgatgtgacg aatagagagc gccgcaccgc cgcgggcatc gccgtccact
     9301 ttggtcaaca ctacaccggt aagcggtaac gcttcattga atgcttttgc cgtattggcc
     9361 gcatcctgac cggtcatggc atcgaccaca aatagggttt caaccggatt aatcgacgca
     9421 tggacttgtt tgatctcgtc catcatcgct tcgtcaacgt gcagacgacc ggcagtatcc
     9481 accagcagca cgtcgtagaa tttcagtttg gcttctttca gcgccgcgtt aacgatatct
     9541 accggcttct gaccaacatc agaagggaag aaatcaacgc ccacctgctc tgccagcgtc
     9601 tcaagctgtt tgattgccgc cgggcgatag acgtcggcag aaaccaccag tactttcttc
     9661 ttgtgcttct cgcgcaggaa cttaccgagt ttaccaacgc tggttgtttt accggcacct
     9721 tgcaggcccg ccatcagcac gaccgcaggc ggttgcgcag ccaggttcag ggtctggttc
     9781 tcttcgccca tcgccgcaac cagttcgtta cggacaattt tgacgaactc ctgccccggc
     9841 gtcaggctct tattaacttc atgaccaacc gctttctctt ttacgcgatt gataaactca
     9901 cgcactaccg gcagagcgac gtcagcctcc agcagcgcca tgcgcacttc gcgcagcgta
     9961 tcttttacgt tgtcttcagt gaggcgtcca cggccactga tattgcgcag cgtgcgcgac
    10021 aaacgatcgg ttaaattatc aaacattgtc tctcgcctgg ggtggaaacg gttggccgca
    10081 atcgcgacac atcatcagta ttttgccgca gtataacatg aaggcgtctt tgttgttatg
    10141 caacggttgg agcagcgttc acctgacgct atactgcttc tctttcttat tgctcaaact
    10201 gtcgacatta ctatgcccgt ttttgctctg ctcgcgcttg tcgcctactc cgtcagtctt
    10261 gcgctgattg ttcccggtct gctgcaaaaa aacggcggct ggcggcgcat ggctattatt
    10321 tctgcggtca tcgcgctggt ctgccacgca atcgctctgg aagcccgcat cctgcccgac
    10381 ggtgatagcg gacaaaacct cagcctgctg aacgttggtt cattggtcag tttgatgatc
    10441 tgtacggtaa tgaccattgt ggcttctcgc aatcgtggct ggttgttgct acccattgtc
    10501 tatgcctttg cgcttatcaa cctagcgctg gcgaccttta tgcccaatga atacatcacc
    10561 catctggaag caacgccggg gatgctggtg cacattggct tatcgctttt ttcctatgcc
    10621 acgctaatta tcgctgccct gtacgcgctg caactggcgt ggattgatta ccaactgaag
    10681 aacaagaagc tggcgtttaa ccaggaaatg ccgccattga tgagtatcga gcgtaaaatg
    10741 ttccatatca cgcagattgg cgtggtgctg ctaacgctca ctctttgcac tggcctgttc
    10801 tacatgcaca acttatttag catggaaaat atcgacaagg ccgtgctctc tatcgtggcg
    10861 tggtttgtct atattgtgct gctgtgggga cattatcatg aaggatggcg tggacgccgc
    10921 gtcgtctggt ttaacgttgc gggtgcagtc attctgacac tggcctactt cggcagccga
    10981 atttttcagc agttaatcag ctaaacccag aaaaggagtt tcccctggaa cacatttcta
    11041 ctactacgtt gatcattatt ctgatcatca tggtggtcat ttcagcctat ttttccgggt
    11101 ccgaaaccgg aatgatgacc ctcaaccgct atcgtctgcg gcatatggcg aaacagggta
    11161 atcgctcggc caaacgcgtc gaaaaattgc tgcgtaagcc agaccgcctg ataagcctgg
    11221 tgttaatcgg caataacctg gtcaatattc ttgcctccgc gctcggcact attgttggaa
    11281 tgcgtttgta cggcgatgcg ggcgtggcaa ttgcgactgg tgtgctgact tttgtcgtgc
    11341 tggtttttgc tgaggtattg ccaaaaacca ttgccgcgct gtacccggaa aaagtcgctt
    11401 atccgagtag ttttctgctg gctccgctgc aaattttgat gatgccgctg gtctggttgc
    11461 tgaatgctat tacccgcatg ttaatgcgca tgatgggcat caaaaccgat atcgtggtta
    11521 gtggctcttt aagcaaagaa gaattacgca ccattgtgca cgaatcgcgg tcacaaattt
    11581 cccgtcgcaa tcaggatatg ctgctgtcgg tactcgatct ggaaaagatg accgtcgatg
    11641 acatcatggt gccgcgcagt gaaattatcg gtattgatat caacgatgac tggaaatcga
    11701 ttctgcgcca actctcccac tcacctcacg ggcgcatcgt gctctaccgt gattcgctgg
    11761 acgacgccat cagtatgctg cgtgtgcgtg aagcctggcg actcatgtcg gagaaaaaag
    11821 agttcaccaa agaaaccatg ctacgcgccg cggacgagat ctattttgtc ccggagggta
    11881 cgccgctcag cacacagttg gtgaagtttc agcgcaacaa aaagaaagtc ggcctggtcg
    11941 tcaacgagta tggagacatt caggggctgg tgacggtcga agatattctc gaagagattg
    12001 tcggcgattt caccacgtcg atgtcgccaa cacttgccga agaggtcacg ccgcaaaacg
    12061 acgggtcggt gattatcgat ggcaccgcca acgtgcgaga aattaacaaa gcctttaact
    12121 ggcatctacc ggaagatgat gctcgtactg ttaacggcgt cattctggaa gcgctggagg
    12181 agattccggt agcaggcacc cgcgtgcgta taggcgagta cgatatcgat attctcgacg
    12241 tacaggacaa tatgattaag caggtaaaag tttttcctgt aaaaccgctg cgcgagagtg
    12301 tggcggagta aaaaaacggc ccggcattcg catgctggac cgtgatttat tacgaaaaca
    12361 gaaattaagc ttttgccttc gctacagtca ccatcgccgc acgaattgta cgaccgttca
    12421 gcgtataacc cttctgcata atgcccagta cgttacctgg cgcaacgtca tcagattcca
    12481 ccattgcgat ggcctgatgc acattcgggt ccagtggtac gttagtttcg gcgatcactt
    12541 caacgccaaa cttacgcaca acatccagca tcgacttcag cgtcagctca ataccttcaa
    12601 ccatcgcgga catatccggg ttagctttat cagccacttc cagcgcacga tccaggctat
    12661 caatcaccgg cagcaattcg ttgatgaatt tctccagcgc gaatttgtga gctttttcaa
    12721 tatccagttc agtacgacga cgcaggtttt ccatttcggc ttttacgcgc aaaatgccgt
    12781 cacgttcacg ggtctgggct tcagccagct gagcttcgag attcgcaatt ttttcatcgc
    12841 gcggatccac ctgctcagca gaagcttctg gctcaactgc ctcaatctct tcgtgctgat
    12901 ccatgataat ttcttccggg gcttgcccct caggcgtttt ctgttcttta ctactcatga
    12961 atttctccgc gtttttttcg cattcatctc gctaacttcg cttattatgg ggatcagttt
    13021 cagggtttca agggaagcac tcacattgtc atcaatcttc gcaacaagga cctcggaaaa
    13081 atgaataatc atttcaagtg tattggcatt gtgggacacc cacggcaccc cactgcactg
    13141 acaacacatg aaatgctcta ccgctggctg tgcacaaaag gttacgaggt catcgttgag
    13201 caacaaatcg ctcacgaact gcaactgaag aatgtgaaaa ctggcacgct cgcggagatt
    13261 gggcaacagg ctgatctcgc ggttgtcgtt ggcggcgacg gtaatatgct gggcgcggcg
    13321 cgcacgctcg cccgctacga tattaaagtt attggaatca accgtggcaa cctgggtttc
    13381 ctgactgacc ttgatcccga taacgcccag caacaattag ccgatgtgct ggaaggccac
    13441 tacatcagcg agaaacgttt tttgctggaa gcgcaagtct gtcagcaaga ttgccagaaa
    13501 cgcatcagca cggcgattaa cgaagtggta cttcatcctg gcaaagtagc gcatatgatt
    13561 gagttcgaag tgtatatcga cgagatcttt gcgttttcgc agcgttccga tggactgatt
    13621 atttcgacgc caacaggctc caccgcatat tccctctctg cgggcggtcc tattctgaca
    13681 ccctctctgg atgcgattac cctggtgccc atgttcccgc atacgttgtc agcacgacca
    13741 ctggtcataa acagcagcag cacgatccgt ctgcgttttt cgcatcgccg taacgacctg
    13801 gaaatcagtt gcgacagcca gatagcactt ccgattcagg aaggtgaaga tgtcctgatt
    13861 cgtcgctgtg attaccatct gaatctgatt catccgaaag attacagtta tttcaataca
    13921 ttaagcacca agctcggctg gtcaaaaaaa ttattctaat tttacgccag cctctttact
    13981 gtatataaaa ccagtttata ctgtacacaa taacagtaat ggtttttcat acaggaaaac
    14041 aactatgttg gcacaactga ccatcagcaa ctttgctatc gttcgtgagc ttgagattga
    14101 ttttcatagc ggcatgaccg taataactgg cgagaccggc gcgggtaaat ctattgcaat
    14161 agatgccctc ggcctttgtc tcggtggtcg cgctgaagcc gacatggtgc gtaccggcgc
    14221 tgctcgtgcc gacctgtgcg cccgtttttc tctgaaagat acgccagcgg ccctgcgttg
    14281 gctggaagaa aaccagcttg aagacgggca tgaatgtttg cttcgtcgcg taatcagcag
    14341 cgatggtcgc tcccgtggtt tcatcaacgg tacagctgtt cctctgtcac aactgcgcga
    14401 actgggtcag ttgctgattc agatccatgg tcagcacgct catcagttac tcaccaaacc
    14461 cgagcaccaa aaattcctgc ttgatggcta tgccaatgaa acctctcagc tccaggaaat
    14521 gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac ctcgcgcatc atcagcagtt
    14581 aagtcaggaa cgcgccgccc gtgcggagct gctgcaatac caattaaaag aacttaacga
    14641 atttaatccg cagcctggag agtttgagca aatcgacgaa gagtacaaac gtctggcgaa
    14701 cagcggtcaa ttgctgacca ccagccagaa tgcattggca ttaatggctg acggtgaaga
    14761 cgcaaatctg caaagtcaac tttacacggc taaacaactg gtgagcgaat tgattggcat
    14821 ggacagcaaa ctgtccggcg tacttgatat gctggaagag gctaccatcc agattgctga
    14881 agccagtgat gaactacgcc actactgcga tcgtctggat ctcgatccca accgactgtt
    14941 tgaacttgaa cagcgcatct caaaacagat ttcgctggca cgtaaacatc acgtcagccc
    15001 tgaggcattg ccacagtatt accagtcgct actggaagaa cagcagcaac tggacgatca
    15061 ggccgactca caagaaacgc tcgcgctggc ggtaacgaaa catcatcagc aggcactgga
    15121 aacggcgcgc gcgttacacc agcaacgcca gcattatgca aatgagttgg cacagctgat
    15181 caccgatagt atgcatgcac tctctatgcc acatgggcag tttacgatcg atgttaaatt
    15241 tgacgagcat cacctgggcg ctgacggcgc tgatcgtatt gagtttcggg taaccaccaa
    15301 cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc ggtggtgagt tgtcccgcat
    15361 cgcattggca atccaggtca ttaccgcgcg taaaatggaa accccagcac tgatttttga
    15421 tgaagtggat gtagggatta gcggcccaac agcggcagtt gtcggcaaac tgctacgtca
    15481 acttggcgaa tcaactcagg tgatgtgtgt tacccacctg ccacaagtcg cgggatatgg
    15541 tcatcaacac tattttgtca gcaaagaaac cgatggtgcg atgacagaaa cgcatatgca
    15601 atccctggat aaaaaagcgc ggttacaaga gttggcgcgc ctgcttggtg gcagtgaagt
    15661 cacacgtaac acactggcga atgcgaaaga actgcttgcg gcgtaaactt ttttcctgct
    15721 tcacggtcag agtaaacagc aaaacgccgt aagaccggaa agcaaaaggt tttaaagtga
    15781 tgaaaggtct attatcatcg gcatattaca gatgagccac gtactgctcg ggcccgaaaa
    15841 ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc agcagcagta ctattgatgt
    15901 tgaccgcagg ctgttccact ctggagcgag tggtttaccg tcctgacatc aaccagggga
    15961 actatctgac cgctaacgac gtatccaaaa tacgtgttgg catgacgcaa caacaagttg
    16021 cgtacgcatt gggtacaccg ctgatgtccg atccatttgg tacaaatacc tggttctatg
    16081 tcttccgcca gcaacctggt catgaaggtg taacgcagca aacgctgacg ctgaccttta
    16141 acagtagcgg tgtgttgacc aacattgata acaaacctgc gctgagtggt aactaataat
    16201 aaagttgctc tcaaagacg
//
LOCUS       DAOFSV010000100        16097 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.100, whole genome shotgun sequence.
ACCESSION   DAOFSV010000100 DAOFSV010000000
VERSION     DAOFSV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.100"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            9..1142
                     /locus_tag="QBH27_003296"
     CDS             9..1142
                     /locus_tag="QBH27_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDQ7015576.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            1231..1764
                     /gene="blc"
                     /locus_tag="QBH27_003297"
     CDS             1231..1764
                     /gene="blc"
                     /locus_tag="QBH27_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDQ7015577.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1761..2078)
                     /gene="sugE"
                     /locus_tag="QBH27_003298"
     CDS             complement(1761..2078)
                     /gene="sugE"
                     /locus_tag="QBH27_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDQ7015578.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2254..2400)
                     /gene="ecnB"
                     /locus_tag="QBH27_003299"
     CDS             complement(2254..2400)
                     /gene="ecnB"
                     /locus_tag="QBH27_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDQ7015579.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2511..2636)
                     /gene="ecnA"
                     /locus_tag="QBH27_003300"
     CDS             complement(2511..2636)
                     /gene="ecnA"
                     /locus_tag="QBH27_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDQ7015580.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2688..3254)
                     /gene="efp"
                     /locus_tag="QBH27_003301"
     CDS             complement(2688..3254)
                     /gene="efp"
                     /locus_tag="QBH27_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDQ7015581.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3296..4324
                     /gene="epmB"
                     /locus_tag="QBH27_003302"
     CDS             3296..4324
                     /gene="epmB"
                     /locus_tag="QBH27_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDQ7015582.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4559..5428
                     /locus_tag="QBH27_003303"
     CDS             4559..5428
                     /locus_tag="QBH27_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDQ7015583.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5478..5831)
                     /locus_tag="QBH27_003304"
     CDS             complement(5478..5831)
                     /locus_tag="QBH27_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDQ7015584.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(5969..7615)
                     /gene="groL"
                     /locus_tag="QBH27_003305"
     CDS             complement(5969..7615)
                     /gene="groL"
                     /locus_tag="QBH27_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDQ7015585.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(7659..7952)
                     /locus_tag="QBH27_003306"
     CDS             complement(7659..7952)
                     /locus_tag="QBH27_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDQ7015586.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8228..9484
                     /gene="yjeH"
                     /locus_tag="QBH27_003307"
     CDS             8228..9484
                     /gene="yjeH"
                     /locus_tag="QBH27_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDQ7015587.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(9500..9976)
                     /gene="fxsA"
                     /locus_tag="QBH27_003308"
     CDS             complement(9500..9976)
                     /gene="fxsA"
                     /locus_tag="QBH27_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDQ7015588.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            10313..11749
                     /gene="aspA"
                     /locus_tag="QBH27_003309"
     CDS             10313..11749
                     /gene="aspA"
                     /locus_tag="QBH27_003309"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDQ7015589.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            11867..13168
                     /gene="dcuA"
                     /locus_tag="QBH27_003310"
     CDS             11867..13168
                     /gene="dcuA"
                     /locus_tag="QBH27_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDQ7015590.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            13284..13622
                     /gene="cutA"
                     /locus_tag="QBH27_003311"
     CDS             13284..13622
                     /gene="cutA"
                     /locus_tag="QBH27_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDQ7015591.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            13598..15295
                     /gene="dsbD"
                     /locus_tag="QBH27_003312"
     CDS             13598..15295
                     /gene="dsbD"
                     /locus_tag="QBH27_003312"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDQ7015592.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            15332..15907
                     /locus_tag="QBH27_003313"
     CDS             15332..15907
                     /locus_tag="QBH27_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ7015593.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            16014..16089
                     /locus_tag="QBH27_003314"
     tRNA            16014..16089
                     /locus_tag="QBH27_003314"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16047..16049,aa:Phe,seq:gaa)
BASE COUNT         3954 a         4138 c         3951 g         4054 t
ORIGIN      
        1 cagaccctat gttcaaaacg acgctctgca ccttattaat taccgcctct tgctccacat
       61 ttgccgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
      121 aacaaaggat ccccggtatg gcggtagcgg taatttatca gggtaaacct tattacttta
      181 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
      241 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
      301 aaatcaagtt aagcgatccc acaacaaaat actggcctga acttaccgct aaacagtgga
      361 atgggatcac actattacat ctcgcaacct acaccgctgg cggcctgcca ttgcaggtgc
      421 cggatgaggt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
      481 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
      541 ctgtgaagcc gtctggtttg agttttgagc aggcgatgaa aactcgtgtc ttccagccac
      601 tcaaactcaa tcatacgtgg attaatgtac cgtccgcaga agaaaagaac tacgcctggg
      661 gatatcgcga aggtaaggca gtacatgttt cgccaggggc gttagatgct gaagcttatg
      721 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta aaaccccttg
      781 atatcaatga gaaaatactt caacaaggga tacaactggc acaatctcgc tactggcaaa
      841 ccggcgatat gtatcagggt ctgggctggg aaatgctgga ctggccggta aatcctgaca
      901 tcatcattaa cggcagtgac aataaaattg cactggcagc acgccccgta aaaccgatta
      961 cgcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     1021 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     1081 aaaactatcc caatccagcg agagttaccg ccgcctggca gattctcaac actctacagt
     1141 aaaatttcat cgggtccgaa ttttcggacc ttttctccgc ttttccttgc tgtcatctac
     1201 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     1261 acagctgcat ttctggtcgt tgcctgcagt tctcctacac cgccgcgtgg cgtgaccgta
     1321 gtgaataatt tcgacgctaa acgctatctc ggcacctggt atgagattgc ccgttttgat
     1381 caccgctttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     1441 ggcctgaatg tcattaataa aggctataac cctgacagag aaatgtggca gcagagtgaa
     1501 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     1561 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     1621 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     1681 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     1741 tgggtacagc agcccggtag ttaatgagtg ctgagtttca gaccaataat ccccaatacg
     1801 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcaa cacaatgccg
     1861 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt
     1921 aacgatttca tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt cacggtaata
     1981 acacttggcg tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact
     2041 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaaattgggg
     2101 ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     2161 attttgccag gtggcagggg aatttcaact attacctatg taactatcgc agaaaaaaca
     2221 aacggcacga cacagctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     2281 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     2341 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     2401 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     2461 catcgccaaa aatgacggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     2521 gcgcgggaga tggagttgcc gaggtgtttg atatcttcgc cgaaacctcg agcggtgtta
     2581 cagcccgtaa gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     2641 aacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     2701 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacaaacagc
     2761 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     2821 tcacctttca ggcccggatc ggtatccacg atttccagtt caacgaagtt cggcggagta
     2881 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcaaccat
     2941 ttggcgttgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     3001 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     3061 gcaccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     3121 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     3181 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     3241 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     3301 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     3361 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     3421 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     3481 catggagaaa ggcaatccga acgatccgct tttgcgtcag gtaattacct cgcaagatga
     3541 gtttgttgtc gcgcccggat tctccaccga cccgctggaa gaacagcaca gcgtagtgcc
     3601 aggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     3661 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     3721 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     3781 ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc tcacacaact
     3841 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     3901 ggcacgcatc acagatgcgc tggttgaacg cttttctcat tctacgctgc aaatcttgct
     3961 ggtgaatcac attaaccatg ccaatgaggt agatgaaaca ttccgtcagg cgatggctaa
     4021 gttgcgccgg gtaggcgtta ctttgctgaa ccagagcgtt ctgttacgtg gtgtgaacga
     4081 taacgcgcaa acgctggcaa acctgagtaa tgcgttgttc gatgcgggtg tgatgccgta
     4141 ttacctgcat gtgctcgata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     4201 agcacggcag attatgcgtg agttgctgac actggtgtcg ggatatctgg tgcctaaact
     4261 ggcgcgagaa attggcggcg aacccagcaa aacaccgctg gatctccagc tacgccagca
     4321 gtaactgatt aaacagggaa gtttaatctt tgcccgttgc ataaccttta attattcata
     4381 tacattgctg ttattactat ttatataacc gacaccaata attaaccttt cttttctgtt
     4441 tatatagagt gagcacggct atttatgtta ttttaacaac tattactacc aagcccattt
     4501 atctggctta taaactaaaa ttaattattt aattgagaaa tggttaggga gaacctacat
     4561 ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc
     4621 actggcgctg aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgccat
     4681 ggaattgcgc gaccttctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga
     4741 tgctgctgca cgccttcagt atgaacaagc tcgcgataaa gtcattaaga aaatggcaga
     4801 aaatatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa
     4861 cacgctggaa cttaccgatg atcagggcga aaatcttacc ttcgtattaa cactacatga
     4921 cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat
     4981 cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt
     5041 agatttcctg ccgttatatg atgtcgactg ccaggatgat ggtaatctgg aatatgacac
     5101 ctattcccaa ccggaatgga aacataactt gttcaaccat tatctggcgg tgctttaccg
     5161 ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc
     5221 aacgccgggt aaagaagtgg aggcgatcac tcgccgaatg cttgatttca gcccccgact
     5281 gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg caaataacgc
     5341 ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac
     5401 cagcaaaact gcaccacagg cgaaataatc ttatctgttt tactacgagg ctggaagtat
     5461 ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat ctggctatcc
     5521 gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta
     5581 ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgcct
     5641 tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgttgcagt accaatcagc
     5701 tggcactctg cacctggttg ctcgtccaca atgcgtacac tctgaccggc agcactcaat
     5761 tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat
     5821 tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc
     5881 tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt
     5941 ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc
     6001 ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg gtaaccatgc
     6061 actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta
     6121 ctttggttgg atccaggata cccatatcga tcatgttgcc gtattcttca gttgctgcgt
     6181 tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc aacaacagac ggttcttcgc
     6241 cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac
     6301 ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca
     6361 gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg
     6421 cgtcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca ccaactttga
     6481 taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca cggtcgtagt
     6541 cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt
     6601 cttcacccac gccatcgatg atggtggtgg tgtctttgtt gatcacaaca cgtttagcct
     6661 gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca gagattacgg
     6721 taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg
     6781 gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca
     6841 gtgcttcgcc ttcaacatct tcagcgatga tcagcagcgg tttacctgct ttggcaacag
     6901 cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga
     6961 acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaagt
     7021 agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt
     7081 cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac
     7141 ctacggtttc gtcggagtta gcagagatgg taccaacctg agcaatcgct ttagagtcag
     7201 agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaacagct ttgtcgatac
     7261 cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagaccc tcagtgatga
     7321 tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtca ttcgctttag
     7381 aggcaacttc tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc agttcgattt
     7441 cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa
     7501 ctacgttacg gcctttagga ccgagggtaa ctttcactgc atctgccagt acgtttacgc
     7561 cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct
     7621 ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga
     7681 atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg
     7741 tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca
     7801 ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct
     7861 gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg
     7921 atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta
     7981 tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg
     8041 atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg
     8101 gtgaaaaccc tgattcacct cacatttcat cgctatttct tcatcaccgt cataagcaaa
     8161 tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggaca
     8221 tcaacccatg agtggactca aacaagaact ggggctggcc cagggcatcg gcctgctatc
     8281 gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg ttagctgcgc tagtagcagg
     8341 caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt
     8401 gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc gtcgcgcact tcgtcggtat
     8461 ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg tcattcccgt
     8521 gggtttgcct gccgcgctac aaattgctgc cggattcggt caggcaatgt ttggctggca
     8581 tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt atatcggtac
     8641 tcgcggtgcc agttccagtg ctaatctaca aacagttatt gccggactta tcgtcgcact
     8701 gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct tccctgcgcc
     8761 aggaaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt
     8821 cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc
     8881 tcgtgctttg atgattggcc tgctgctggc aggattagtc tattggggct gtacggtagt
     8941 cgtcttacac ttcgacgcct atggtgaaca aatggcggcg gcagcatcgc ttcccaaaat
     9001 tgtagtgcag ttgttcggtg taggagcgtt atggattgcc tgcgtaattg gctatctggc
     9061 ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc
     9121 gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc
     9181 cctcaatgcg gtgctcggct gctgcgtggt aagcacgttg gtgattcatg ctttagagat
     9241 caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg
     9301 catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg
     9361 gctattatgc gttctgttac tggcaatggt cggctggaaa agtctctacg cgctgatcat
     9421 gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac
     9481 ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg cggtcgcggt
     9541 catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa
     9601 aaccgccgcc tggcatgcga gaaaagcgca aatgcggcat caacttcact gtcagatgct
     9661 tttgcaccgg cggcaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata
     9721 aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc gctgggtttt
     9781 cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca
     9841 gtgacatacc gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata
     9901 catgagcaac ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa
     9961 aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gttaaacccg gctacctgaa
    10021 tgggttgcga atcgcgttta gcttatattg tggtcattag caaaatttca agatgtttgc
    10081 gcaactattt ttggtagtaa tcccaaagcg gtgatccatt tcacaaatta ataattaagg
    10141 ggtaaaaacc gacacttaaa gtgatccagg ttacggtaga aatcctcaag cagcatatga
    10201 tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat
    10261 atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa
    10321 caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta
    10381 tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga
    10441 tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa
    10501 agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt
    10561 cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg
    10621 tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg
    10681 tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc
    10741 tactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaaact
    10801 ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga
    10861 catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga
    10921 attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac gtaccgctga
    10981 actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa
    11041 agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc
    11101 ggctgaagac ctgatcgaag cgacctccga ctgcggcgct tatgttatgg ttcacggcgc
    11161 gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc
    11221 tggtccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcag gctcttccat
    11281 catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt
    11341 catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt
    11401 tatggagcca gtcattggcc aggccatgtt cgaatccgtt cacattctga ccaacgcttg
    11461 ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg
    11521 ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa
    11581 cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct
    11641 ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat
    11701 gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt
    11761 agtacaaata aagaaggcac gtcagatggc gtgccttttt tcttgtgagc agtaacttaa
    11821 aaataacaat ttaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga
    11881 actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg
    11941 ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat
    12001 cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt
    12061 tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa
    12121 atacatcacg atcctcgcac cgatcgtgac ctatttcctg actatctttg ctggtactgg
    12181 caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa
    12241 accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc
    12301 aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct
    12361 ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtccttcct
    12421 ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga
    12481 agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac
    12541 gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag
    12601 cccaagcatg ggtctggttg aaaaaccact gatgaacacc accaacgcaa tcctgatcat
    12661 catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacattct
    12721 caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg
    12781 gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt
    12841 gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta
    12901 ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttctcc
    12961 gctgaccgct gttgcttctt ttgctgcggt gtctggtctg ttcattctgc cgacctaccc
    13021 gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg gtaaattcgt
    13081 cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg
    13141 cttcgtgctg ggtagcttca tgctgtaatg acccatcgcg gggcgttcac gccccgcttt
    13201 ctttcccgcc gactaacatc ctttccccgt ccgttgtata gtgacctctc tcttgcagtt
    13261 ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg
    13321 tggtgctatg tacggcaccg gatgaagcga cagcccagga tttagccgcc aaagtgctgg
    13381 cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc tattactggg
    13441 aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc gtatctcacc
    13501 agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg gaacttctgg
    13561 ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca tctttacgct
    13621 gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc cgggacgttc
    13681 acaatttgtc cccgcggatc aagcctttgc ttttgatttt cagcaaaacc aacatgacct
    13741 taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga tccgcattac
    13801 gccggaacac gcgaaaattg ctgacgtgca gctgccgcaa ggcgtctggc atgaagatga
    13861 gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa ccatcaacca
    13921 ggcaagtgcg ggagcaacgt taactgtcac ctaccagggc tgtgctgatg ccggtttctg
    13981 ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca acgaagcgtc
    14041 acagcctgtg tctgttccgc agcaagagca gcccaccgcg caattgccct tttccgcgct
    14101 ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc
    14161 actgatttct ggcatcgtgc tgggcggtaa acagcggctt tccactgcca gagcattgtt
    14221 gctgaccttt atttatgtgc aggggatggc gctgacttac acggcgctgg gtctggtggt
    14281 tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc tcattggcct
    14341 cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc tgcaactccc
    14401 ctcttcgctg caaacacgtc tcacgctgat gagcaatcgc caacagggcg gctcacctgg
    14461 cggtgtgttt attatggggg cgattgccgg actgatctgt tcaccatgca ccactgcacc
    14521 gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg gcggcggcac
    14581 gctttatctt tatgcgctgg gcatgggcct gccgctgatg ctaattaccg tctttggtaa
    14641 ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt
    14701 gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatatat ggggattacg
    14761 cttgtggtcg gcgcttggtg tcgcattctt tggctgggcc tttatcacca gcctacaggc
    14821 caaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat tggttagcgt
    14881 acgcccactt caggattggg catttggtgc aacacatacc gcgcaaactc agacgcatct
    14941 caactttaca caaatcaaaa cggtagatga gttaaatcag gcgctcgttg aagccaaagg
    15001 caaaccagtg atgttagatc tctatgccga ctggtgcgtc gcctgtaaag agtttgagaa
    15061 atacaccttc agcgactcgc aggtgcaaaa agcgttagca gatacggtct tacttcaggc
    15121 caacgtcact gccaacgacg cacaagatgt ggcgctgtta aagcatctta atgtccttgg
    15181 cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag cacgcgtcac
    15241 gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac
    15301 acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc
    15361 cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg ttgctgaacg
    15421 tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg caatcctcta
    15481 cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc tgatgctgga
    15541 cgaaacgcaa accgccgaac aaaagttgct ggcacgttat caggcgttat cggagtgcgt
    15601 taaaaacaac cgctatccgg gctgtctgtt tatcgctgcc tgtacgtttt atcccgatcc
    15661 tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg atttcaccca
    15721 cgaactgtta accacgctgg aagttgacga tccggcgatg gtagcaaagc agatggaact
    15781 ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac
    15841 cgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgtcagg gtggtgcact
    15901 gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt
    15961 ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc gttgcccgga
    16021 tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg attccgagtc
    16081 cgggcaccaa attcata
//
LOCUS       DAOFSV010000101        15701 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.101, whole genome shotgun sequence.
ACCESSION   DAOFSV010000101 DAOFSV010000000
VERSION     DAOFSV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.101"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            246..581
                     /locus_tag="QBH27_003315"
     CDS             246..581
                     /locus_tag="QBH27_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDQ7015594.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            741..1184
                     /gene="yjdN"
                     /locus_tag="QBH27_003316"
     CDS             741..1184
                     /gene="yjdN"
                     /locus_tag="QBH27_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDQ7015595.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1317..2105
                     /gene="phnC"
                     /locus_tag="QBH27_003317"
     CDS             1317..2105
                     /gene="phnC"
                     /locus_tag="QBH27_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015596.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2130..3146
                     /gene="phnD"
                     /locus_tag="QBH27_003318"
     CDS             2130..3146
                     /gene="phnD"
                     /locus_tag="QBH27_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7015597.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3251..4030
                     /gene="phnE"
                     /locus_tag="QBH27_003319"
     CDS             3251..4030
                     /gene="phnE"
                     /locus_tag="QBH27_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDQ7015598.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4051..4776
                     /gene="phnF"
                     /locus_tag="QBH27_003320"
     CDS             4051..4776
                     /gene="phnF"
                     /locus_tag="QBH27_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDQ7015599.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4777..5229
                     /gene="phnG"
                     /locus_tag="QBH27_003321"
     CDS             4777..5229
                     /gene="phnG"
                     /locus_tag="QBH27_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDQ7015600.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5226..5810
                     /gene="phnH"
                     /locus_tag="QBH27_003322"
     CDS             5226..5810
                     /gene="phnH"
                     /locus_tag="QBH27_003322"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDQ7015601.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5810..6874
                     /gene="phnI"
                     /locus_tag="QBH27_003323"
     CDS             5810..6874
                     /gene="phnI"
                     /locus_tag="QBH27_003323"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDQ7015602.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6867..7712
                     /gene="phnJ"
                     /locus_tag="QBH27_003324"
     CDS             6867..7712
                     /gene="phnJ"
                     /locus_tag="QBH27_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDQ7015603.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7709..8467
                     /gene="phnK"
                     /locus_tag="QBH27_003325"
     CDS             7709..8467
                     /gene="phnK"
                     /locus_tag="QBH27_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDQ7015604.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8578..9258
                     /gene="phnL"
                     /locus_tag="QBH27_003326"
     CDS             8578..9258
                     /gene="phnL"
                     /locus_tag="QBH27_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDQ7015605.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9255..10391
                     /gene="phnM"
                     /locus_tag="QBH27_003327"
     CDS             9255..10391
                     /gene="phnM"
                     /locus_tag="QBH27_003327"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDQ7015606.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGKIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVEREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10391..10948
                     /gene="phnN"
                     /locus_tag="QBH27_003328"
     CDS             10391..10948
                     /gene="phnN"
                     /locus_tag="QBH27_003328"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDQ7015607.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10935..11369
                     /gene="phnO"
                     /locus_tag="QBH27_003329"
     CDS             10935..11369
                     /gene="phnO"
                     /locus_tag="QBH27_003329"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDQ7015608.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11371..12129
                     /gene="phnP"
                     /locus_tag="QBH27_003330"
     CDS             11371..12129
                     /gene="phnP"
                     /locus_tag="QBH27_003330"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDQ7015609.1"
                     /translation="MSLILTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12157..12513)
                     /locus_tag="QBH27_003331"
     CDS             complement(12157..12513)
                     /locus_tag="QBH27_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015610.1"
                     /translation="MKRFPLFLLFTLFTLFTLFTLFTLFTVPAQADIIDDTIGNIQQA
                     INDAYNPDRGRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDERQRQLDQER
                     QQLEDEERRMEDEYGR"
     gene            complement(12582..13031)
                     /gene="rpiB"
                     /locus_tag="QBH27_003332"
     CDS             complement(12582..13031)
                     /gene="rpiB"
                     /locus_tag="QBH27_003332"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDQ7015611.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13390..14280
                     /gene="alsR"
                     /locus_tag="QBH27_003333"
                     /gene_synonym="rpiR"
     CDS             13390..14280
                     /gene="alsR"
                     /locus_tag="QBH27_003333"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDQ7015612.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14339..15274
                     /gene="alsB"
                     /locus_tag="QBH27_003334"
     CDS             14339..15274
                     /gene="alsB"
                     /locus_tag="QBH27_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDQ7015613.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATETFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15402..>15701
                     /locus_tag="QBH27_003335"
     CDS             15402..>15701
                     /locus_tag="QBH27_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ7015614.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLEN"
BASE COUNT         3710 a         4461 c         4231 g         3299 t
ORIGIN      
        1 gtcagaaaga taaaaacatc atgtgaatta aaaaaagaac gagtagagca ttaaccttat
       61 cttaaataat aaatggaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa
      121 acgatcgtca tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg
      181 cggccttttt cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa
      241 tccacatgtc attaccacac tgcccaaaat gcaactccga atacacctac gaagataacg
      301 gcatgtacat ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa
      361 gcgacgagct gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta
      421 ccatcattaa agatctgaaa gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag
      481 taaaaaacat ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg
      541 gtccgatgaa actgaagtct gagtttgtga aaaagaactg attgtattgt gatcggtagg
      601 ccggataagg cgctcgcgcc gcatccggca acggtggcga ctgcctgatg cgacgctgac
      661 cgcgtcttat caggcttccc tctcatctgt ataaatttcg aactacactt aactggcttc
      721 tcttaactga ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtt
      781 ccgacgcgat tgcctattat caacgtacgt tgggcgcgga actgctctat aaaatcagct
      841 tcggcgaaat gccaaaatca acgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat
      901 ttcccgatac cgccatcgcc cacgccaatg tgcgcattgc aggaagcgac atcatgatga
      961 gcgatgctat accgtcagga aaagccagct actccggctt tacgctggtg ctcgattctc
     1021 aacaggtgga agaaggaaaa cgctggtttg ataaccttgc cgccaacgga aaaatcgaaa
     1081 tggcctggca ggaaacgttc tgggcgcatg gctttggcaa agtcaccgat aaatttggcg
     1141 taccgtggat gattaatgtc atcaaacaac agccaacgca ataacccgcc gggaggcccg
     1201 ccctcccgca ctgtcatcga attcccgtta actcttcatc tgttagtcac ttttaattaa
     1261 ccaaatcgtc acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc
     1321 aaacgattat ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcag gcgctgcacg
     1381 cggttgatct gaacattcat catggtgaaa tggtggctct gcttgggccg tcgggttccg
     1441 gaaaatccac ccttttacgt cacttaagcg gtttgattac cggcgataaa tctgtcggta
     1501 gccatatcga gctgctgggc cgcacagtcc agcgcgaagg ccgcctggcc cgcgatatcc
     1561 gcaaaagccg cgccaacacc ggctacatct ttcaacaatt caacctggtg aatcgcctga
     1621 gcgtactgga gaacgtgctg attggcgcgc tcggcagcac gccattctgg cgaacctgtt
     1681 ttagctactt cacccgcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca
     1741 tggtgcactt tgcccatcaa cgcgtttcca cactctccgg cggccagcaa cagcgtgtgg
     1801 cgattgcccg cgcgctgatg cagcaggcga aggtcattct cgctgacgaa cccatcgcct
     1861 cgctggaccc ggaatcggca cgcatcgtga tggacaccct gcgcgacatc aaccagaacg
     1921 acggcattac cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac
     1981 gcatcgtcgc cctgcgccag gggcacgtct tctacgacgg ctgcagccaa cagtttgata
     2041 acgaacgttt tgaccatctc taccgcagca ttaaccgcgt cgaagagaac gcgaaagctg
     2101 cctgacatcc ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct
     2161 tcaccagcat gttcagcctc agcaccctgt taaatccggc atacgccgaa gagcaggaaa
     2221 aggcgctgaa tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga
     2281 cgccgttctt acaggatatg gagaagaagc tgggcgtaaa ggtcaacgcc ttctttgccc
     2341 cggactacgc gggcattatc caggggatgc gcttcaataa agtggatatc gcctggtacg
     2401 gcaacctgtc ggcgatggaa gcggtggatc gcgccaacgg tcaggtcttc gcccagacgg
     2461 tcgcggcgga tggatcgccg ggttactgga gcgtgttgat cgtcaacaaa gatagcccga
     2521 tcaacaacct gaacgatctg ctggcgaagc ggaaagatct caccttcggc aatggcgatc
     2581 ctaactctac ctccggcttc ctcgtccccg gttactacgt cttcgctaaa aacaatatct
     2641 ccgccagcga ctttaagcgc accgtcaacg ccgggcatga aaccaacgcc ctcgccgtcg
     2701 ctaacaagca agtggatgtc gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct
     2761 ccgcgccgga gaagctgaaa gaactgaagg tgatctggaa atcaccgctg atccctggcg
     2821 atccgatcgt ctggcgcaaa aatctttccg aaaccactaa agacaagatc tacgacttct
     2881 ttatgaatta cggcaaaacg ccggaagaga aagcggtgct ggagcgcctg ggctgggcgc
     2941 cgttccgcgc ctccagcgac ttgcaactgg tgccgattcg ccagctcgca ctgtttaaag
     3001 agatgcaaag cgtgaaggac aacaaagggc tgaacgaaca ggacaagctg gcaaaaacca
     3061 ccgccattca ggcgcaactg gatgatttgg accgcctgaa caacgcgtta agcgcaatga
     3121 gttcggtgag taaagcggtg cagtaaatcg taggtcggat aagacgcccg gcgtcgcatc
     3181 cgacaatgtg cagacgttga tgccggatgc ggtgcaagca ccttatccgg cctacagacc
     3241 ggagccaaac atgcaaacca tcaccatcgc cccacccaag cgcagctggt tctcgcttct
     3301 gagctgggcc gttgttatcg ccgtgctggt cgtctcgtgg cagggcgcgg aaatggcccc
     3361 gcttacgctg attaaagatg gcggcaacat ggcgacgttc gccgccgact tcttcccgcc
     3421 cgatttcagc cagtggcagg attacctcac cgaaatggcc gtcacgctgc aaatcgccgt
     3481 ctggggcacc gcgctggcgg tggttctctc catccccttc ggcctgatga gcgctgaaaa
     3541 cctggtaccg tggtgggtct accaacctgt acgccgcctg atggacgcct gccgcgccat
     3601 taacgaaatg gtcttcgcca tgctgttcgt ggtcgccgtc ggcctcggcc cgttcgctgg
     3661 cgtgctggcg ctgtttatcc acaccaccgg cgtgctctcc aagctgcttt ccgaagcggt
     3721 ggaagcgatc gaacccggcc cggtggaggg cattcgcgcc accggtgcca acaagctgga
     3781 agagatcctt tacggcgtac taccgcaggt catgccgctg ctgatttcct actctctcta
     3841 tcgcttcgaa tccaacgtcc gctcggcgac cgtcgtcggt atggttggcg cgggcgggat
     3901 cggcgtcacg ctgtgggaag cgattcgcgg tttccagttc cagcaaacct gcgccctgat
     3961 ggtgctcatc atcgtcacgg tcagcctgct ggatttcctc tctcaacggt tgcgtaagca
     4021 ctttatctga taagcgaggc attgatatct atgcacttgt ctacacatcc gaccagctac
     4081 ccaacacgct atcaggagat agccgcaaaa cttgagcagg agcttcgtca acactaccgc
     4141 tgcggcgact atcttcccgc cgagcagcaa ctggcggcgc gctttgaggt gaaccgccac
     4201 accctgcgcc gcgccatcga ccaactggtg gaaaaaggct gggtacagcg ccgtcagggc
     4261 gtcggcgtac tggtgctgat gcgcccgttc gattacccgc tcaacgccca ggcgcgtttt
     4321 agccagaacc tgctggacca gggcagccat cccaccagcg aaaaattgct atcggtgctg
     4381 cgccccgcct ccggtcacgt cgctgacgcg ctggggataa ccgaggggga gaacgtcatc
     4441 cacctgcgca ccctgcgtcg ggtcaacggc gtcgcgctct gtttaatcga ccactacttt
     4501 gcggacctca ccctctggcc gacgctgcaa cgtttcgaca gcggttcgct gcacgatttt
     4561 ctgcgcgagc aaacggggat tacgctacga cgcagtcaga cgcggatcag cgcccgtcgc
     4621 gcccaggcca aagagtgcca gcgtcttgaa atcccgaatc tgtcgccgct gctgtgcgtg
     4681 cgcaccctta accaccgtga cggtgaaagc agcccggcgg agtactccgt cagcctgacg
     4741 cgcgccgaca tgattgaatt cactatggag cactgaatgc acgcagatac cgcgacccgc
     4801 cagcactgga tgtccgtgct ggcgcacagc caaccggctg aactggcagc ccgcctgaac
     4861 gcgctgaaca tcaacgccga ttatgaggtg atccgcgccg ctgaaactgg cctggtgcag
     4921 attcaggcgc gaatgggcgg caccggcgaa cgcttttttg ctggcgacgc cacgctcacc
     4981 cgcgccgccg tgcgtctgac tgacggcacg ctcggctata gctgggtgct ggggcgcgac
     5041 aaacagcacg ccgaacgttg tgcgctgatt gatgcgctga tgcagcaaaa ccgccacttt
     5101 caaaacttat cagaaaccct tatcgccccg ctggacgctg accgtatggc acgcattgcc
     5161 gcgcgccagg ccgaagtgaa cgccagccgg gtcgacttct ttacgatggt tcgcggagac
     5221 aacgcatgac cctggaaacc gcttttatgc ttcccgtgca ggatgcccag cacagttttc
     5281 gtcgcctgtt aaaggccatg agcgagccgg gcgtgattgt cgccctgcat cagctcaagc
     5341 gcggctggca gccgctgaat atcgccacca ccagcgtgct gctgacgctg gccgataacg
     5401 acacgccggt gtggcttgcc gcgccattga gcaacgatat cgtcaatcag agcctgcgtt
     5461 ttcataccaa cgcgtcgctg gttaatcagc cggaacaggc gaccttcgcg gtaacagatg
     5521 aggcgatctc cagcgagcag ctcaacgccc tttccaccgg cactgccgtt gcaccagaag
     5581 cgggcgcaac gctgatttta caggtcacca gcctgagcgg cgggcgtatg ttgcgcctca
     5641 ccggcgcagg tattgccgaa gaacggatga tcgccccgca actgccaaag tgcattcttc
     5701 atgaacttac cgagcgcccg cacccgttcc cgctcggcat cgacctgatc ctgacctgcg
     5761 gcgagcgcct gctggctatt ccgcgaacca ctcatgtgga ggtgtgctga tgtacgttgc
     5821 cgtgaaaggg ggcgaaaagg cgatcgacgc cgcccacgcc ctgcaagaga gccgacgccg
     5881 gggcgatact gatttgcctg aactgagcgt cgcccagatt gaacagcagc ttaacctcgc
     5941 ggtagatcgc gtgatgaccg aaggcggtat tgccgaccgc gagctggccg cgctggcgct
     6001 gaaacaggcc agcggcgata acgtcgaagc gatttttctg ttgcgcgcct accgcaccac
     6061 gctggcgaag ctggcggtca gtgagccgct cgacaccacc gggatgcgtc tcgaacggcg
     6121 tatttcagca atttataaag acattcccgg cggccaactg cttggcccca cctacgacta
     6181 cacccatcgc ctgctcgatt tcaccctgct ggcaaacggc gaaacgccaa cgctgaccac
     6241 cgctgacagc gaacagcaac cgtcgccaca cgtcttcagc ctgctggcgc gtcaggggct
     6301 ggcgaagttt gaagaagata gcggcgcaca accggacgac atcacccgca cgccgccggt
     6361 ttatccctgt tcgcgttctt cccgtctaca acaattaatg cgcggtgacg aaggctattt
     6421 gttggcgctg gcctactcca cccaacgcgg ttacgggcgc aatcacccgt tcgcaggcga
     6481 aattcgcagt ggctatatcg atatatttat tgtgccggaa gagctgggat ttgcggtgaa
     6541 cgtcggcgaa ttgttgatga ccgagtgtga aatggtcaac ggttttatcg acccgccgga
     6601 tgaaccaccg cattttacac gcggctatgg gctggtgttc ggcatgagcg agcgcaaagc
     6661 aatggcgatg gcgctggttg accgtgcgtt gcaggcaccg gaatacggcg agcacgcaac
     6721 aggtccggca caggatgaag agttcgtgct ggcccatgcc gacaacgtcg aagccgcagg
     6781 ctttgtttcg catctcaaac tcccgcacta cgtcgatttc caggccgaac tggagctact
     6841 caaacgtctg caacaggagc agaaccatgg ctaatctgag cggctacaac tttgcttacc
     6901 tcgacgagca gaccaaacgc atgatccgcc gcgctatctt aaaagcggtg gcaatccccg
     6961 gttatcaggt gccgtttggc ggacgcgaga tgccgatgcc ctacggctgg ggaaccggcg
     7021 gcattcagct caccgccagc gtgattggcg aaagcgacgt gttgaaggtg attgaccagg
     7081 gcgcagacga caccaccaac gccgtgtcga ttcgtaactt ctttaagcgc gtgaccgggg
     7141 tgaacaccac cgagcgcacg gacgatgcga cacttatcca gacgcgtcac cgcatacccg
     7201 aaacgccgct gaccgaagat cagatagtta tcttccaggt gccgatcccc gaaccgctgc
     7261 gctttattga gccgcgtgag acggaaacgc gcaccatgca cgcgctggag gagtacggcg
     7321 tgatgcaggt gaagctgtat gaagatatcg cccgcttcgg tcatatcgcc accacctacg
     7381 cctatccggt gaaggtgaac gggcgctacg tgatggaccc gtcgccgatc ccgaaattcg
     7441 ataacccgaa aatggacatg atgcccgccc tgcaactgtt tggcgcgggg cgcgagaagc
     7501 gcatctatgc cgtgccaccg tttacccgcg tggaaagcct cgatttcgat gatcatccgt
     7561 tcaccgtcca gcagtgggat gaaccatgcg ccatctgcgg atcgacccac agctatcttg
     7621 atgaagtggt gctggatgac gccggaaacc gcatgtttgt ctgctccgat accgattatt
     7681 gccgccaaca gagcgaggcc aaaagccaat gaatcaaccg ttactttcgg tcaataacct
     7741 gacccacctt tatgccccgg gcaaaggctt cagcgatgtc tcttttgatt tatggccggg
     7801 agaagttctg ggcattgttg gggagtccgg ttccgggaag accacgctac tgaaatcaat
     7861 ttccgcccgc ctgacaccac agcaagggga aattcgctac gagaaccgtt cgctgtacgc
     7921 gatgagcgag gccgaccgcc gtcgcctgct gcgcaccgaa tggggtgtgg tgcatcagca
     7981 tccgcttgac ggcctgcgcc gccaggtgtc ggcaggcggc aatatcggcg aacggttgat
     8041 ggcaaccgga gcgcgtcatt acggcgatat tcgcgccacc gcgcagaaat ggctggaaga
     8101 ggtggagatt cccgccaacc ggatcgacga cctgccgacc accttttccg gcggcatgca
     8161 gcaacgtttg cagattgccc gcaacctggt gacgcatccg aagctggtgt ttatggatga
     8221 acccactggc gggctggatg tgtcggtaca ggcgcgcctg ctggacctgc tccgcggtct
     8281 ggtgatggag cttaacctgg cggtagtgat tgtcacccac gatttaggcg tcgcccgtct
     8341 gctggcggac cgtttgctgg taatgaagca agggaaagtg gtggagagtg ggttaaccga
     8401 ccgcgtgctc gacgacccgc accatccgta tacccagttg ctggtgtcat cggttttgca
     8461 gaattgagct ggtgccggat gcggcgtaaa cgccttatcc ggcctacaaa tgcgctcccc
     8521 gtaggtcgga taagacgcat cagcgtcgca tccgacaccc gaaccacgag gcgaaaaatg
     8581 attaacgtac aaaacgtcag taaaaccttc atcctgcacc agcaaaacgg cgtgcgcctg
     8641 cctgtattac aaggcgcctc actcaccgtc aacgccggcg aatgcgtggt actccacggc
     8701 cattccggca gtggaaaatc aactctgcta cgctcgctgt atgccaacta tctacccgac
     8761 gaagggcaaa tccagatcaa acacggtgat gaatgggtcg acctggtcac cgcgccagcg
     8821 cgcaaagtgg tggagatccg caaaaccact gtcggctggg taagccagtt tctgcgcgtc
     8881 atcccacgta tttcggcact ggaagtggtg atgcagccgc tgctcgatac cggcgtaccg
     8941 cgtgaagcct gcgccgctaa agccgcgcgt ctcctcaccc gcctgaacgt gcctgaacgc
     9001 ctgtggcacc ttgcgccatc gaccttctcc ggtggcgaac agcagcgtgt caacatcgcc
     9061 cgtggcttta tcgtcgacta ccccattctg ctgctcgacg aacctaccgc ctcgctggac
     9121 gctaaaaaca gcgccgccgt agtggaactg atccgcgaag ccaaagcccg cggcgcggcc
     9181 atcgtcggta tcttccatga cgaaaccgta cgcgagcaag tcgccgaccg cctgcaccca
     9241 atgggagtct cttcatgatt atcaataacg tcaaactggt gctggaaaac gaggtggtac
     9301 acggttctct ggagatgcag gacggcaaaa tccgcgcctt tgccgaaagc cagagtcacc
     9361 tgtcagaagc aatggacggc gaaggcggct ggctgctacc ggggctgatt gagctgcata
     9421 ccgataatct ggataaattc ttcaccccgc gcccgaaagt tgactggcct gcccactcgg
     9481 cgatgagcag ccacgacgcg ctgatggtgg cgagcggcat caccaccgtg ctggacgccg
     9541 tggcgattgg tgatgtacgc gacggcggcg atcggctgga gaatctggag aagatgatca
     9601 acgccatcga agagacgcag aaacgcggcg tcaaccgtgc cgagcaccgt ctgcacctac
     9661 gctgcgaact gccgcatcac accacgctgc cgctgtttga aaaactggtc gagcgagaac
     9721 ccgtgacgct ggtgtcgctg atggatcact caccgggcca gcgccagttc gccaaccgcg
     9781 aaaaatatcg tgaatattat cagggcaaat actcgctcac cgatgcgcag atgcaacagt
     9841 acgaagagga gcaactggcg ctcgccgcac gctggtcaca gccgaatcgc gaatccatcg
     9901 ccgcaatgtg ccgcgcgcga cacattgcgc tcgccagcca cgatgacgcc acgcacgccc
     9961 acgtagccca atcccatcag cttggcagcg tgatcgccga atttcctacc acattcgaag
    10021 cggcagaagc ctcgcgcaag cacggcatga acgtactgat gggcgcgccg aatatcgtgc
    10081 gcggcggttc gcactccggc aacgtggcgg ccagtgaact ggcgcagctt ggcctgctgg
    10141 atatcctctc ttccgactac taccccgcca gcctgctgga tgcggcgttt cgcgtggccg
    10201 atgaccagag caaccgcttt acgctaccgc aggcggtgag gctggtgacc aaaaacccgg
    10261 cccaggcact gaatctccag gatcgcgggg tgattggcga gggcaaacgc gccgacctgg
    10321 tgctggcgca tcgcaagggc aatcacattc atattgacca cgtctggcgt cagggtaaaa
    10381 gggtgttctg atgacgggaa aactgatttg gttaatgggt ccgtccggct ccgggaaaga
    10441 cagcctgctg gcggaactcc gccagcggga acaaacccag ctactggtag cgcatcgcta
    10501 catcacgcgt gatgccagcg ccggaagtga aaatcatatc gccttaagcg agcgggagtt
    10561 ttttacccgc gcggggcaaa atctgttggc cttgagctgg catgctaatg gtttgtatta
    10621 cggtgtcggc attgagatcg acctctggct gcacgccgga ttcgacgtcg tggttaacgg
    10681 ctcacgcgcc cacctgccgc aggcgcgggc acgttatcaa tcggcactgc tgcccatctg
    10741 tttacaggtt tcgccggaga tcctgcgcca gcgcctggaa aaccgtggcc gcgaaaacgc
    10801 cagtgaaatt aacgcccggc tggcgcgcgc cgcccgctat actcctcagg attgtcttac
    10861 gctcaataat gacggcagcc tgcgccagtc ggtcgacaag ctgctgacgc tgattcatca
    10921 gaaggagaaa caccatgcct gcttgtgagc ttcgcccggc tacgcagtac gacaccgacg
    10981 cagtttacgc gctgatttgt gagctaaaac aggcggagtt tgaccaccac gcgtttcgcg
    11041 tggggtttaa cgccaatctg cgcgatccga acatgcgcta ccatctggcg ctgctggatg
    11101 gcgaagttgt cggcatgatc ggcctgcatt tgcagtttca tctgcatcat gtcaactgga
    11161 tcggcgaaat tcaggagttg gtggtgatgc cgcaggcgcg cggcctgaac gtcggtagca
    11221 agttgctggc gtgggcagaa gaagaagccc gccaggccgg ggccgaaatg accgagcttt
    11281 cgaccaacgt gaagcgccac gacgcgcacc gtttctatct gcgcgaaggc tacgagcaga
    11341 gccatttccg cttcaccaaa gcgctgtaac atgagcctga tcctcacgct caccggcact
    11401 ggcggcgcac agggcgttcc cgcatggggc tgtgagtgcg cggcctgcgc cagagcgcgg
    11461 cgctcaccgc agtatcgccg ccagccatgc agcggcgtcg tgaagtttaa cgacgcgatc
    11521 accctgatcg acgccgggct gcacgatctc gccgatcgct ggtcgcccgg atcgttccag
    11581 cagtttttgc tgacgcacta ccatatggat cacgtgcagg ggctgtttcc gctgcgctgg
    11641 ggcgtgggcg acacaatccc ggtctacggt ccaccggatg agcagggctg cgacgatctg
    11701 tttaaacatc cgggcctgct tgatttcagc cacacggtgg agccgtttgt ggtgtttgat
    11761 ttgcaggggt tgcaggtcac gccgctgccg cttaaccatt cgaaactcac tttcggctat
    11821 ttactggaaa cggcgcacag ccgggtggcg tggctatctg acaccgcagg cctgccggaa
    11881 aaaacgctga aatttttacg caataatcag ccgcaggtaa tggtgattga ttgcagccac
    11941 ccgccgcgcg aggatgcacc gcgtaatcac tgtgatttaa ataccgtgct tgcgctgaat
    12001 caggttatcc gctcgccacg ggtgattctg acccatatca gccaccagtt tgatgcgtgg
    12061 ctgatggaaa atgcactgcc gtcggggttt gaggcggggt ttgatgggat ggagattgag
    12121 gtggagtgag ggaaaatacc cgcgccggcc cctacctcaa cgcccatact catcttccat
    12181 tctccgctct tcatcttcca gttgctgacg ctcctgatca agctggcgct ggcgttcgtc
    12241 cagctgcctg cggcgatctt caaactggcg gcggcggtca tcatattgtc tgcgccgatc
    12301 gtcgctcact tcacgctgcc agccgtcgtc gcgcgaatct tcatagtctc gcccacggtc
    12361 agggttataa gcgtcgttaa tcgcctgctg aatattgcca atggtgtcat cgataatatc
    12421 ggcctgggcc ggaacggtga acagcgtgaa cagcgtgaac agcgtgaaca gcgtgaacag
    12481 cgtgaataaa agaaatagcg gaaagcgttt cattagccaa cctcaaaaag aaactctatc
    12541 cacattagtc attactcatc catgcaagta gtggatgaat ctcaatttct ccgctgctct
    12601 attgccgtaa tcgcctccac gcgttgttga tgacgaccgc cttcgtactg tgcgcccagc
    12661 cacgcatcga caatcatttt tgccagttcg aggccaacta ctcgtgaacc aaaagccagc
    12721 acgttggtgt cgttatgctg ccgcgaaagt tgcgcggaat aaggttcgct acaaacgacc
    12781 gcgcgaattc cggcaaactt gttcgccgct atcgaaatac cgacgccagt gccacaaatc
    12841 aaaatcccgc catcagcctc tccgccagca acggccagtg cgacttgact ggcgtaatgc
    12901 ggataatcag tacgctctga cgaccaggtt cctttatcaa tcacttcaac gccacgctca
    12961 actaaatgtg ccactatttc atgttttaaa atgaaaccga catgatcaca gccaaatgca
    13021 atctttttca ttcatcttcc ccgctcgcgc aaaacttcac aataaaaaaa catttgtgat
    13081 ttaaatgaat taaaaacacc caaaatcgat gaaatcagcc ttcccgatct gttttacaga
    13141 gttacatctg gcagcgcaat agcgttaaag acactcacta tccggcaaca tctatcatct
    13201 aataaaccag aaaaacaaat aacatcatgt ttttaaagtg attaaatgaa ataaaatttt
    13261 aagccactct ccattattca caataaaaca aactttataa attttatttt tttgtgaagt
    13321 tgccagcatc ttttctgttc ttgctgtggt gatatagtgg cgtcttcaat tcaaggacaa
    13381 gagaacgtga tgagccagtc agagtttgat tcagcgcttc cgaacggtat cgggttagcg
    13441 ccttacctgc gaatgaagca ggaaggaatg acagaaaacg aaagccgcat cgtggagtgg
    13501 ttactcaaac ccggtaacct gagttgtgca cccgcaatta aagatgtcgc agaagctctg
    13561 gcggtatctg aagcgatgat cgttaaggta tcaaagctgc tggggtttag cggctttcgt
    13621 aacttacgca gtgcgctgga agattatttt tctcagtcag aacaagtatt gccgtccgag
    13681 ttggcttttg atgaagcgcc gcaggatgtg gtgaataagg tatttaacat cactttacgc
    13741 accattatgg aaggtcagtc gatcgtcaac gttgatgaga tccaccgtgc cgcccgcttt
    13801 ttctatcagg ccagacagcg ggatttgtac ggtgccggag gatcaaatgc aatctgtgct
    13861 gatgtacagc acaagttttt acgcattggc gtacgctgcc aggcctatcc ggacgctcac
    13921 atcatgatga tgtctgcttc gttgttacag gaaggagatg tggtgctggt ggtgacccat
    13981 tccgggcgaa ccagtgatgt aaaagcagct gtagaactgg caaaaaagaa cggggcaaag
    14041 attatttgta taacgcatag ctaccattca ccgatagcga agctggccga ttatattatt
    14101 tgctcaccgg ccccggaaac accgttatta ggtcgtaatg cctcggcaag aatattacaa
    14161 ctaactttgc tggacgcgtt ttttgtctct gtcgcccagc tcaacattga acaagctaat
    14221 attaatatgc aaaaaaccgg cgcaattgtt gatttcttct caccaggcgc gctgaaataa
    14281 gaaaaaggta attccgccat cgcggagtta cctatcccct taccgtgaga acatgattat
    14341 gaataaatat ctgaaatatt tcagcggcac actcgtgggc ttaatgttgt caaccagcgc
    14401 ttttgctgcc gccgaatatg ccgtcgtatt gaaaacactc tccaacccat tttgggtaga
    14461 tatgaaaaaa ggcattgaag atgaagcaaa aacgctgggc gtcagcgttg atatttttgc
    14521 ctctccttca gaaggcgatt ttcaatctca attgcagtta tttgaagatc tcagtaataa
    14581 aaattacaaa ggtatcgcct tcgcaccatt atcctcagtg aacctggtca tgcctgtcgc
    14641 ccgcgcatgg aaaaaaggca tttatctggt caatctcgat gaaaaaatcg acatggataa
    14701 tctgaaaaaa gcaggcggta atgtggaagg ttttgtcacc accgataacg ttgccgtcgg
    14761 ggcgaaaggc gcgtcattca ttattgacaa gctgggtgcc gaagggggtg aagttgcaat
    14821 tattgaaggt aaagccggta acgcctccgg tgaagcgcgt cgtaatggtg ccaccgaaac
    14881 cttcaaaaaa gcaagccaga tcaagcttgt cgccagccag cctgccgact gggaccgcat
    14941 taaagcactg gatgtcgcca ctaacgtgtt gcaacgtaat ccgaatatta aagcgatcta
    15001 ttgcgcgaat gacacgatgg caatgggtgt tgctcaggct gtcgcaaacg ccggaaaaac
    15061 gggaaaagtg ctggtcgtcg gtacagatgg cattccggaa gcccgcaaaa tggtggaagc
    15121 cggacaaatg accgcgacgg ttgcccagaa cccggcggat atcggcgcta cgggtctaaa
    15181 gctgatggtt gacgctgaaa aatccggcaa ggttatcccg ctggataaag caccggaatt
    15241 taaactggtc gattcaatcc tggtcactca ataaaactaa atgatgccgg atagtgatgc
    15301 taatgaagtg tcttatccgg ccaacaaaat aacctgaacg tagacctgat aagtgtagcc
    15361 catcaggcaa taatacgata tttcgtgcag agaggttatt tatggccacg ccatatatat
    15421 cgatggcggg gatcggcaag tcctttggtc cggttcacgc attaaagtcg gttaatttaa
    15481 cggtttatcc tggtgaaata catgcattac taggagaaaa tggcgcgggt aaatccacgc
    15541 taatgaaagt gttatccgga atacatgagc caaccaaagg caccattacc attaataaca
    15601 ttaactataa caaactggat cataaattag cggcacaact cggtatcggg attatttatc
    15661 aggaactcag cgttattgat gaattaaccg tactggaaaa t
//
LOCUS       DAOFSV010000102        15505 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.102, whole genome shotgun sequence.
ACCESSION   DAOFSV010000102 DAOFSV010000000
VERSION     DAOFSV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.102"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            307..1128
                     /locus_tag="QBH27_003336"
     CDS             307..1128
                     /locus_tag="QBH27_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015615.1"
                     /translation="MSAIENQETVIQAVNSLHPTTLFHFTKNEEAFYSILTELYFKPF
                     LARERIVGVKGRRNFAVPMVSFCDIKLSQIKDHSGKYGEFGFGLTKEWAEKNDLHPVL
                     YMNQGSELFSKYNKRIRKLKDALIPLWSKRHILDNKERKYFESLKEEYADLYNLLRYM
                     KNYKGKLERTNEKTIENYIYADEKEWRYVPHPFVGDLWPSLNLERVVEPNQKAVLSKK
                     FSEFGIGFSFDDIKYILIPDDSHVSNLINCLMSIRNYDPYIISKVLTMDKVKQDF"
     gene            complement(1667..6430)
                     /locus_tag="QBH27_003337"
     CDS             complement(1667..6430)
                     /locus_tag="QBH27_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ7015616.1"
                     /translation="MVKPNWDNFKAKFSENPQGNFEWFCYLLFCQEFKMPAGIFRYKN
                     QSGIETNPITKDNEIIGWQSKFYDTKLSDNKADLIEMIEKSKKAYPGLSKIIFYTNQE
                     WGQGRKSHEPEGDKNADNYLETVGNSNDPKIKIEVDQKAYESGIEIVWRVASFFESPF
                     VIVENEKIAKHFFSLNESIFDLLEEKRKHTENVLYEIQTNIEFKDRSIEIDRRHCIEL
                     LHENLVQKKIVIVSGEGGVGKTAVIKKIYEAEKQYTPFYVFKASEFKKDSINELFGAH
                     GLDDFSNAHQDELRKVIVVDSAEKLLELTNIDPFKEFLTVLIKDKWQVVFTTRNNYLA
                     DLNYAFIDIYNITPENLVIKNLERGELIELSDNNGFSLPQDVRLLELIKNPFYLSEYL
                     RFYTGESIDYVSFKEKLWNKIIVKNKPSREQCFLATAFQRASEGQFFVSPACDTGILD
                     ELVKDGIVGYEAAGYFITHDIYEEWALEKKISVDYIRKANNNEFFEKIGESLPVRRSF
                     RNWISERLLLDDQSIKPFIAEIVCGEGISNFWKDELWVAVLLSDNSSIFFNYFKRYLL
                     SSDQNLLKRLTFLLRLACKDVDYDLLKQLGVSNSDLLSIKYVLTKPKGTGWQSVIQFI
                     YENLDEIGIRNINFILPVIQEWNQRNKVGETTRLSSLIALKYYQWTVDEDVYLSGRDN
                     EKNILHTILHGAAMIKPEMEEVLVKVLKNRWNEHGTPYFDLMTLILTDLDSYPVWASL
                     PEYVLQLADLFWYRPLKETGERYHSMDIEDEFGLFRSHHDYYPESPYQTPIYWLLQSQ
                     FKKTIDFILDFTNKTTICFAHSHFAKNEIEEVDVFIEEGKFIKQYICNRLWCSYRGTQ
                     VSTYLLSSIHMALEKFFLENFKNADSKVLESWLLFLLRNTKSASISAVVTSIVLAFPE
                     KTFNVAKVLFQTKDFFRFDMNRMVLDRTHKSSLISLRDGFGGTDYRNSLHEEDRIKAC
                     DDVHRNTYLENLALHYQIFRSENVTEKDVIERQQVLWGIFDKYYNQLPDEAQETEADK
                     TWRLCLARMDRRKMKITTKEKDEGIEISFNPEIDPKLKQYSEEAIKKNSEHMKYVTLK
                     LWASYKREKDERYKNYGMYEDNPQIALQETKEIIKKLNEEGGEDFRLLNGNIPADVCS
                     VLLLDYFNQLNNEEREYCKDIVLAYSQLPLKEGYNYQVQDGTTSAISALPVIYHNYPM
                     ERETIKTILLLTLFNDHSIGMAGGRYSVFPSMVIHKLWLDYFDDMQSLLFGFLILKPK
                     YVILSRKIIHESYRQVDYDIKKININKVFLNNYKHCISNVIDNKISIDDLGSIDIVDL
                     HILNTAFQLIPVDTVNIEHKQLVSLIVKRFSTSLLSSVREDRVDYALRQSFLERFAYF
                     TLHAPVSDIPDYIKPFLDGFNGSEPISELFKKFILVEDRLNTYAKFWKVWDLFFDKVV
                     TLCKDGDRYWYVDKIIKSYLFAESPWKENSNGWHTFKDSNSQFFCDVSRTMGHCPSTL
                     YSLAKSLNNIASCYLNQGITWLSEMLSVNKKLWEKKLENDTVYFLECLVRRYINTERE
                     RIRRTKQLKEEVLVILDFLVEKGSVVGYMSRENIL"
     gene            complement(6506..6925)
                     /locus_tag="QBH27_003338"
     CDS             complement(6506..6925)
                     /locus_tag="QBH27_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015617.1"
                     /translation="MAKHLDNRDINAIVNLIRGWKEPKLSWSAICEAAEPLVGKLPSR
                     QSLNAHEAIVTAYQTKKEALKGRGPKNPRPASLNIAAARIVNLESEIEELKEENRRYK
                     QQFVIWQYNAYKYGMTEHQLNAQLTKIDRERSDGERR"
     gene            complement(6918..8951)
                     /locus_tag="QBH27_003339"
     CDS             complement(6918..8951)
                     /locus_tag="QBH27_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDQ7015618.1"
                     /translation="MAEIFLFKPKATLTAAENLEAFISQCRDQLTVFGSDLTWEDPVW
                     PNITVFAKLGIITRKPILEETQDPAFIDFAKAYFRYQQGHSLSRAKNESKALRAVEAA
                     LLQVNGNANIDGLSISVLDEAAELAKHYYSDGSAYHCGREIERLAKFVAENQLVSSAV
                     QNWVNPIKRAEDKNKTSRKAKKNREDKLPSDIALNALAEIFANDPIHERDIFTTSVFA
                     MLMSAPSRITEVLALPVDCEVFETDREGIERYGWRFFAGKGYEGDIKWIPTVMVGIAK
                     TAVARARVLSENARQLAKWIEKHPNKFYRHANCPSVADDEPLTMKQVCMALGFAHYSK
                     KTCRSCLGNRGLAKEDGVHTLNSLWQHTMARLPDDFPWFDKDKGIRYSNALFVLNVNQ
                     FHGNRGCIPVELHKPTNNFFNSDLTPRLSLKGKHASIFDRHGYHAVNGEPVKLTSHQA
                     RHLLNTIIQRGGLSNLEIAKWSGRADVKQNRTYNHMTEYELVGIAERLDPTTALFGPA
                     GEVAKHLPVTMQEFNTLEHAAVHVTEYGYCVHDYTMSPCEKFRDCVNCTEQVCIKGDD
                     AEKLDRIKIRLEKLERLFFLADAAVESGEIGTDRWYQYHKKTVTRLRELVAILENPDI
                     ENGVQIKLRGNDFSQLRRVVAKTSIVAIEQKGKESEEAVMLDDLTTLLGGGLG"
     gene            complement(8968..10506)
                     /locus_tag="QBH27_003340"
     CDS             complement(8968..10506)
                     /locus_tag="QBH27_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDQ7015619.1"
                     /translation="MTMNNLLLGLVPERIRARANGYSFLLYDTSWTLDRNITINLADI
                     HHYLSDGTLDGCLRTLAFYASNRSSSHTKNILERFQHMLRETDAREITDTVLINYRSM
                     LSPATEWYLATIRGFLRSWHELGYPGISKEVNRLLDGWTLKGNRKGDAVKRMDPQEGP
                     LTDNELTAFNEGAVRAYEKSLITIAELAIALGTSHTGRRPIQISLLRVVDVLCGKNGK
                     GEPFYVLNVPRAKQRSSSFRINFKPFAISLELWAILSAQARNAIAQVENRLGFELQED
                     DRQQIPLFPDLDVLDAVQSPYEFRQLHATDKLHIPAAEITDTLQYIVEKSDIRSERTG
                     ELLHINARRFRYTTGTRAAREGFGELVIAELLDHADTQNAGVYIKNIPEHVKKLDEAV
                     GFQLAPYAQAFVGALVDSERDAHRGNDPTSRIRTEMGQGVGTCGEHGFCGANVPIPCY
                     TCMHFQPWLDGPHEDVYQGLLNERERVKEITGDIQIAAVLDRSIIALADVIMRCAKRR
                     EELS"
     gene            complement(10493..11740)
                     /locus_tag="QBH27_003341"
     CDS             complement(10493..11740)
                     /locus_tag="QBH27_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ7015620.1"
                     /translation="MEKNYRVTTFVMDSGERYCMVVDRASGLPMYYPTLFLTTQIRNR
                     EDAFSTMLAAANNLVMLLRFVESRDIDLEQRFLTKDFFKPHELDDLRDFAQCKQRKGP
                     LTVSSTSWLADAMTDIVDNGTQHSRLTTFANYLHWYAMHILKTTELEVVEKINAMAAQ
                     IKTRRPSKKRRNHDLQDRSLSDVQLDALFEVIQPRSELNPFSIDVQRRNRLMILLLFY
                     LGIRGGELLNIRIQDIDFSANRIRIVRRADEQADSRTNEPNAKTRERLLPLAESLAQE
                     LHSYILQDRRKVRNAKKNDYLFVTYKSGPTLGNPISKGGYHKIFSVVRSISPQLYAAT
                     GHSLRHTWNRKFSERMDAMNEQVSEERQEQLRSYLMGWRDGSGTAATYNKRFIRQKGF
                     EAALALQAGNGTSLPEDFKDDHE"
     gene            complement(11899..12435)
                     /gene="ssb1"
                     /locus_tag="QBH27_003342"
     CDS             complement(11899..12435)
                     /gene="ssb1"
                     /locus_tag="QBH27_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDQ7015621.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            12690..>15505
                     /gene="uvrA"
                     /locus_tag="QBH27_003343"
     CDS             12690..>15505
                     /gene="uvrA"
                     /locus_tag="QBH27_003343"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDQ7015622.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPDGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKP"
BASE COUNT         4307 a         3575 c         3172 g         4451 t
ORIGIN      
        1 gaacggttca cataacctga tacttataaa tcgtgaaaaa aaaccttttt tatcagaaac
       61 taaggcataa gaaatgattt aactgtaaag aatttagtta aaatataatc tatcaaagtg
      121 cataatttta ctttttttgg tgacccagct cggacctttg attcaatctg gcatagattt
      181 tggtaagaga tcaaaaaacc ggaaatctca aaaagtgcgt ggaattaaac caagtgtgct
      241 tacagtacaa agactagtat gataaccttt gctaagtcag gttttacact atggaacagt
      301 taatatatga gtgctataga aaaccaagaa acagttatac aagcagttaa tagtttacat
      361 ccaacaacgt tgtttcactt tactaaaaat gaagaggcat tctatagcat tttaacagaa
      421 ctctatttta aaccatttct tgcccgtgaa cgcatagtcg gagtaaaagg aagaagaaat
      481 ttcgcagtac caatggtttc attctgtgac atcaagcttt ctcagattaa agatcatagt
      541 ggaaaatatg gtgaatttgg atttgggtta acaaaagaat gggctgagaa aaatgatctc
      601 catcccgttc tatacatgaa tcaaggcagt gaattatttt caaagtataa taaaagaatt
      661 agaaaactaa aggatgcgtt aattccgcta tggtcgaaaa ggcatatcct tgataataaa
      721 gagcgtaagt attttgaaag ccttaaagaa gagtacgctg acctttataa tttattacgc
      781 tatatgaaaa attataaagg gaaattagaa aggacgaacg aaaaaaccat tgagaattat
      841 atctacgctg acgaaaaaga atggagatat gtaccgcatc cgtttgttgg cgatttatgg
      901 cctagcctaa atttagagcg agtggtggaa cctaaccaga aggccgtctt aagtaaaaaa
      961 ttctctgaat ttggcattgg tttttctttc gatgatatta aatatatact tattcctgat
     1021 gatagccacg tttccaattt gataaattgc cttatgagta taagaaatta tgatccttat
     1081 ataataagta aagttttaac gatggataaa gtaaaacagg atttctaaca cttctacata
     1141 gaaattccat taataaacaa gaaattagct ttattgagga cagctttacc taaagctgaa
     1201 aagtgctaaa tatcaaactt tcatcccata agctatgata tagcactcac tttattgata
     1261 aattccgagg tattcatgaa aggtcagatc gatatcgaac tccaaagatt gatacgggcg
     1321 cggtataaca ccgcggtaga aaattggagt gaatggaaat ggttagaaat cgggcatggc
     1381 gtcgtcatca tatatctcgg ttaaaatcta agagaaaaac atatcgaatt gcgttggcta
     1441 aaactgaaaa agttgtaggg aaaatttact gcaccccttg tgcttgttct tgttatttat
     1501 gtggtaatca aagaatttat cacggagtga acattcaaga gcaacgagct ttgctgcgtt
     1561 ttactgattg acttataaga tattaatctt ttcgataata aaaaggctcc aatccctgga
     1621 gcctaaattg gaaaagctct cattaaaatt attattttca actacatcac agaatatttt
     1681 cccgtgacat ataaccaaca acagatcctt tctctaccaa aaaatccaat attactaaga
     1741 cctcttcttt caactgtttg gttcgtctaa ttcgctcacg ctcagtgtta atataccgcc
     1801 taaccaaaca ttctaaaaaa taaacagtat cattttccaa tttcttttcc catagctttt
     1861 tattaaccga caacatttct gaaagccagg ttataccttg attaagatag caactggcaa
     1921 tgttattcaa agatttggca agagaatata aagttgaagg gcaatggccc atagtcctag
     1981 atacatcgca aaagaattga ctattgctat ctttaaatgt gtgccaacca ttagagtttt
     2041 ctttccatgg agattcagca aaaaggtaac tttttataat tttatctaca taccaatacc
     2101 tatctccatc cttgcacaaa gtaaccactt tatcaaaaaa caaatcccaa accttccaaa
     2161 atttggcgta agtatttaat ctatcttcga cgagaataaa ttttttaaat aactctgaaa
     2221 taggctctga accgttgaaa ccatcaagaa aaggttttat ataatcggga atatcgctca
     2281 cgggcgcatg aagcgtaaag taggcaaatc tttccaagaa agactgccga agagcgtaat
     2341 caactctatc ttctcgaaca cttgacaata ggcttgtaga aaatctttta acaattaagg
     2401 aaaccaattg cttatgttca atattaacag tatcaactgg aattaattgg aaagctgtgt
     2461 tcaaaatatg tagatcaact atatctatac ttcccaaatc atctatagat attttattat
     2521 cgatgacatt tgatatgcaa tgcttatagt tatttaaaaa caccttatta atatttattt
     2581 ttttaatgtc atagtctact tgacgataac tttcatgaat gatttttctt gaaagaatta
     2641 catactttgg ctttaaaatc aaaaaaccaa acaatagaga ctgcatatca tcaaaatagt
     2701 ctagccataa tttatgaatc accatactag gaaatactga gtagcgccca cctgccattc
     2761 caatagagtg gtcattaaac agtgtcaaaa gtaatattgt ttttatagtc tccctttcca
     2821 ttggataatt atgataaatc acgggtaagg ctgaaattgc cgaggttgtc ccatcttgta
     2881 cctgataatt atagccttcc ttcaacggaa gttgagaata tgctagaaca atatctttac
     2941 aatattctct ctcttcatta ttcaactgat taaagtaatc taacagtaat acagaacaaa
     3001 cgtctgctgg tatattacca tttaatagtc tgaaatcttc acccccttcc tcattaagct
     3061 tttttattat ttctttggtc tcttgtaaag caatttgcgg attgtcctca tacattccat
     3121 aattcttata acgttcatcc ttttctcttt tatagcttgc ccatagtttc agcgttacat
     3181 acttcatatg ctcggagttt ttctttattg cttcctcact atattgcttt agtttagggt
     3241 caatctcagg attgaatgat atctcaatcc cttcatcttt ctccttggta gttattttca
     3301 tctttcgccg atccattctt gccaagcaga gcctccacgt cttatcggct tcagtttctt
     3361 gagcttcatc tggaagctga ttatagtatt tgtcgaaaat accccagagc acttgttgcc
     3421 tttcaatgac atctttctcc gttacatttt cactcctgaa aatttgataa tgcaaggcaa
     3481 gattttcaag ataagtattt ctatgcacat catcgcaagc tttaattcta tcttcttcgt
     3541 gcaaagagtt tctgtaatct gtaccgccaa agccatccct gagggagatt aatgaacttt
     3601 tatgtgttct gtctagaacc attcgattca tatcaaaacg gaagaaatcc tttgtttgga
     3661 atagtacttt agctacattg aatgtcttct cagggaatgc aagtacaata ctcgtaacta
     3721 ctgcagaaat agaagctgac ttggtatttc ttaacaagaa aagaagccaa ctttccaaca
     3781 ctttcgagtc tgcattttta aaattctcaa gaaaaaattt ttccaatgcc atatgaattg
     3841 atgaaagtaa gtaggtagag acctgtgttc ctcgatatga gcaccacaga cgattgcata
     3901 tatattgctt tataaacttt ccttcttcaa taaagacatc gacttcttca atttcgtttt
     3961 tagcaaaatg ggagtgggca aaacatatcg ttgtcttgtt cgtaaaatca agaataaagt
     4021 ctattgtttt tttgaactgt gattgtagta accaatatat aggagtctga tatggacttt
     4081 ctggataata gtcgtgatga gacctaaata gaccgaactc atcttcaata tccatactgt
     4141 gataacgttc gcctgtttct ttaagtggcc gataccagaa cagatctgcc aattgtagaa
     4201 catattccgg gagagatgcc caaaccggat atgaatctaa gtcagtaagg attaaggtca
     4261 taaggtcgaa atatggggta ccgtgctcgt tccacctatt tttaagaacc ttaactaaaa
     4321 cctcttccat ttcaggttta atcatggccg ccccatgaag aatcgtatgc agaatatttt
     4381 tctcattatc ccttccggat aaatagacat cctcatctac agtccattga taatatttta
     4441 gggctatcaa actagataat cgagtcgttt cacccacttt gtttctttga ttccactcct
     4501 gaatcacagg aagtataaaa ttaatatttc tgatccctat ttcatctaaa ttttcataga
     4561 taaattggat cacactctgc caaccagttc ccttaggctt agtaagaaca tatttaatgg
     4621 aaagcagatc tgaattactt acacctaact gtttaagcag atcgtaatca acgtccttgc
     4681 aagcaagcct caataagaaa gtaagtcttt ttaatagatt ctggtcacta ctaagtaaat
     4741 atcttttaaa gtaattaaaa aatatgcttg aattatcgga aagaaggaca gctacccata
     4801 actcgtcttt ccaaaaattt gatattcctt ctccacagac tatttctgcg ataaaaggct
     4861 ttatggactg gtcatctaaa agcaatcgtt cagatatcca attccgaaaa ctacggcgaa
     4921 caggaagtga ttctcctatt ttttcgaaga actcgttatt gttcgcttta cggatataat
     4981 cgacagaaat tttcttttct aatgcccatt cctcgtatat atcatgtgta atgaagtaac
     5041 cagcagcttc atagccgaca attccgtctt taactaactc atctaaaatt ccagtatcac
     5101 atgccgggga gacaaaaaat tggccctcac tagcccgctg aaaagcagtc gctaagaaac
     5161 actgctcccg agaaggttta tttttgacaa taatcttatt ccaaagcttt tctttgaagc
     5221 tcacataatc gatgctttca ccggtataga acctcaaata ttcacttaga taaaatggat
     5281 ttttgattag ttctaataat cgaacatctt gaggaaggct aaatccattg ttatcagata
     5341 actctattag ctcgccgcgt tcaaggttct ttattactaa gttttcagga gttatattat
     5401 aaatatctat gaaagcatag ttcagatctg ccaagtagtt gttacgggtt gtgaaaacaa
     5461 cctgccattt atcctttatt aaaacagtca ggaattcttt aaaaggatcg atattggtca
     5521 gttctaaaag cttttcagca gaatctacga ctatgacttt acgtaattcg tcttgatgag
     5581 cattagagaa atcgtctaag ccatgcgcac cgaatagctc attaatactg tcctttttaa
     5641 actcgctagc cttaaagaca tagaaaggag tgtattgttt ttctgcttca taaatttttt
     5701 tgataactgc tgtttttcca accccacctt ctccgctgac gatgacaatt tttttctgaa
     5761 ctagattctc atgtagaagt tctatacaat gtcgtctgtc aatttcaata cttctgtctt
     5821 tgaactctat attggtttga atttcatata aaacattttc tgtgtgcttg cgcttttctt
     5881 ctaataaatc aaagatgctt tcattaaggg agaagaaatg tttagcaatc ttttcattct
     5941 caactattac aaacggtgat tcaaaaaaac tagcaactct ccatactatt tcgatacccg
     6001 actcatatgc tttctgatca acttcaattt ttattttggg atcgttacta tttccgacag
     6061 tttccaaata attatcagcg ttcttatcgc cttcaggttc atgggacttt ctcccctgcc
     6121 cccactcttg attagtatag aaaatgattt tacttaatcc tggataagcc tttttgcttt
     6181 tctcaatcat ttctataaga tcagctttgt tatccgacaa ttttgtgtca tagaatttag
     6241 attgccaacc gataatttca ttatctttgg ttattggatt agtttcgata ccagattgat
     6301 tcttatatct aaatatacct gcgggcattt tgaattcttg acagaacaac aagtagcaaa
     6361 accactcaaa attaccttga ggattctcac taaatttagc tttaaagtta tcccagtttg
     6421 gtttgaccat ttaattattc tcctattttt ttgtaaataa ctacccctac acagagaaat
     6481 gtaccctcga tatcgtactc agcaactatc ttctttctcc atcagagcgc tcacggtcaa
     6541 tctttgtcag ctgcgcatta agttgatgtt ctgtcattcc atatttgtat gcattgtatt
     6601 gccagatcac aaactgttgt ttgtacctcc ggttctcttc cttaagctct tcgatttcgc
     6661 tttcaagatt cacaatcctc gcggctgcaa tgttcaaact agctggacgc ggatttttgg
     6721 ggccacggcc tttaagcgcc tctttctttg tttgataggc agtcactatt gcctcatgtg
     6781 cattaaggga ctgtcttgac ggcaactttc cgaccaatgg ttctgccgcc tcacaaattg
     6841 ctgaccagga cagttttggt tccttccatc ctcgaattag atttacgatg gcattaatgt
     6901 ctctattgtc cagatgctta gccaaggcca cctcctaaca aagttgtcaa atcatctaac
     6961 atcacagctt cttcactttc tttgcctttt tgctcgatgg ccacaataga cgtctttgcg
     7021 acaacacggc gaagctggct gaaatcattg ccccggagct taatctggac accattttca
     7081 atgtctggat tctctaaaat agccactagt tcacgaagcc gggtaacagt tttcttgtgg
     7141 tactgatacc agcgatcagt tcctatctca ccactttcca ctgctgcgtc agcaagaaaa
     7201 aacagtcgct ctaatttctc cagacgtatc ttaatgcgat ccaacttttc agcatcatcg
     7261 cctttgatgc aaacctgttc ggtacaattg acgcaatctc ggaatttctc acaaggactc
     7321 atggtgtaat catgcacaca gtagccatac tccgtcacat ggactgcggc atgctctaag
     7381 gtattgaact cctgcatagt cacgggcaga tgctttgcca cctcgccagc aggcccaaat
     7441 agcgctgttg tgggatcaag gcgttcagca atgccaacca gttcgtattc agtcatgtgg
     7501 ttgtaagttc gattctgttt cacatctgcc cgccccgacc acttggcaat ttccaagttt
     7561 gacaggccac cgcgctggat aatggtgttg agcaggtgtc gagcctgatg tgaggttaat
     7621 ttcaccggct ctccgtttac agcatggtaa ccatggcgat caaagatact ggcatgtttt
     7681 cctttcaagg acagacgagg cgtcagatca ctgttgaaga aattattggt tggcttatgt
     7741 aactcaactg gtatgcatcc tctgttcccg tggaactgat ttacgttcag tacaaacaag
     7801 gcattgctgt acctaattcc cttgtcttta tcaaaccacg gaaagtcatc cggtaaacgc
     7861 gccattgtgt gctgccacag gctatttaga gtatgtacac catcctcttt agctaaacct
     7921 cgattaccaa gacacgacct gcacgtcttt ttagagtaat gtgcaaaacc gagcgccata
     7981 caaacctgct tcatcgtcaa tggctcatca tcggctacac tgggacagtt agcatggcgg
     8041 taaaacttat tcgggtgttt ctcgatccac ttggccagtt gtcgtgcatt ttcagagagc
     8101 actctagctc tagctacggc tgtcttagct ataccgacca tcacggtggg tatccatttg
     8161 atatcaccct catagccttt ccccgcaaaa aaacgccaac catatcgttc tatcccttca
     8221 cgatccgttt caaaaacctc gcaatccaca ggcagtgcta acacctcagt aatacgtgaa
     8281 ggtgccgaca tcaacatggc aaaaaccgat gttgtaaata tgtctcgctc atgaattggg
     8341 tcgttggcaa aaatttccgc caacgcatta agagcaatat cactcggaag tttatcttcc
     8401 ctatttttct tcgcctttct gctcgttttg ttcttatcct ctgcacgttt aattggattg
     8461 acccaatttt gtacagcact tgatacaagc tggttttcag ctacaaactt agccagccgc
     8521 tcaatctcac ggccacaatg atatgcggag ccatcggagt agtaatgctt tgccaattca
     8581 gctgcttcat ccagcacaga aatcgacagg ccatcaatat ttgcgtttcc atttacttga
     8641 agcaaagccg cctcaaccgc acggagggct ttactttcat tctttgccct agagaggcta
     8701 tgcccctgct ggtaacggaa ataggctttg gcaaaatcaa tgaaagcagg gtcttgcgtc
     8761 tcttccagta tgggttttct cgtaataatg cccaacttgg caaatacagt tatgtttggc
     8821 caaacaggat cttcccaagt gagatcagag ccgaatacag tcaattgatc tcggcactgg
     8881 gatatgaatg cttcaaggtt ttctgctgct gtcagcgtag cctttggctt aaacaggaaa
     8941 atctcagcca ttggttattg ctccctgtta actaagctct tcacgacgtt ttgcacaacg
     9001 catgatcaca tctgccagcg cgataatact gcgatcaagt acggcagcaa tctggatgtc
     9061 gccagttatc tctttcaccc gttcacgttc gttgagcagt ccttgataga catcttcatg
     9121 tgggccgtcc agccaaggct ggaaatgcat gcaggtgtag caaggaatag gaacattggc
     9181 accacaaaag ccatgttctc cacaagtacc aacaccctga cccatttcgg tccgaatacg
     9241 actggtaggg tcgttacctc gatgcgcgtc tctctctgaa tcgacaagtg ccccgacaaa
     9301 ggcctgagcg tatggtgcta attggaagcc aaccgcctca tcaagctttt tcacatgctc
     9361 ggggatattc ttgatgtaaa cgcctgcgtt ttgggtgtcc gcgtggtcaa gcagttccgc
     9421 aatcaccagc tcgccaaagc cttcccgggc agctcgtgtg cctgtggtat aacgaaaacg
     9481 acgagcattg atatgtaata gctcaccggt acgttccgac cggatgtcgg acttttctac
     9541 gatgtattgg agggtgtcgg ttatctctgc ggcgggaatg tggagtttgt cggtagcatg
     9601 cagttggcgg aattcataag gagattggac ggcatctaac acatcaagat caggaaaaag
     9661 agggatttgc tgccggtcat cttcctgcaa ttcaaacccc aaacgatttt caacctgcgc
     9721 aatcgcattt cttgcctgag cactcaggat tgcccatagt tccagtgata ttgcaaatgg
     9781 tttgaagttt attcggaaag agctgctgcg ctgcttggct cgcggcacat tcaacacata
     9841 gaagggctct ccctttccat ttttaccaca aaggacatcg accacacgaa gcagcgagat
     9901 ctgaatggga cgacgtcctg tatgactggt gccaagtgct atagccaact cagctatagt
     9961 aattaagctc ttttcataag cccgaacagc cccctcgtta aacgctgtca gctcattatc
    10021 cgtcagtggc ccctcttgag gatccatgcg cttaaccgca tcccccttac gattaccctt
    10081 tagcgtccag ccatctagca agcggttcac ctccttactg atgccgggat agcccagttc
    10141 gtgccaggat cgaagaaagc cacggatggt tgctagatac cattctgttg caggcgacag
    10201 catcgaccga taattaatca ataccgtatc agtaatttct ctcgcgtcag tctcacgcag
    10261 catgtgctga aaccgttcaa gaatattttt tgtatgagaa gaagaacgat tggatgcata
    10321 aaacgccagt gtacgcaggc agccatctag cgtcccatct gacaagtaat ggtgaatatc
    10381 agcaagatta atagtgatat ttctatccaa tgtccacgat gtgtcataca acagaaaaga
    10441 gtagccattg gccctggcac ggattcgttc tggtactaac ccaagtaaga ggttattcat
    10501 ggtcatcctt gaagtcctct ggcaagctag tgccgttacc tgcttgtaat gccaaggcgg
    10561 cctcaaaacc tttttgccga atgaaacgct tgttgtaagt cgctgcggtt cccgagccat
    10621 ctctccagcc cattaagtac gaacgaagtt gctcttggcg ttcctcgctg acttgctcat
    10681 tcatggcatc cattctttct gaaaacttgc gattccaagt gtggcggagc gaatgtcctg
    10741 tcgctgcata caattgcggt gatatagacc tgactacgga aaaaatttta tgatatcccc
    10801 ctttggagat aggatttccc agagttggtc cactcttgta agttacaaac agatagtcat
    10861 ttttcttcgc atttctgact ttccggcgat cttgaaggat ataactatgc agttcttgag
    10921 ctaaggattc agccaacggt aagagccgtt ctctagtttt agcattaggc tcattggttc
    10981 tagagtcagc ttgttcatct gctctcctaa cgatacggat cctgttagcg ctaaaatcta
    11041 tgtcctgtat tcttatattt aataattcac cacctcggat gccaagataa aacagaagta
    11101 agatcatcag ccgattgcgg cgctgcacat ctattgagaa agggtttaac tctgatctag
    11161 gctggatgac ttcaaatagc gcgtccaatt gaacatcact caatgacctg tcttgtaagt
    11221 cgtggtttct gcgcttttta gatggtcggc gcgtctttat ctgcgccgcc atagcattga
    11281 tcttttcgac aacttccagt tctgtcgtct ttaagatatg catcgcatac cagtgtaaat
    11341 agttggcaaa cgttgttagc cgggaatgct gtgtcccgtt atcaacgata tcggtcatcg
    11401 catcggccaa ccaagaggta gaggacacag ttaatggccc ctttctttgt ttgcactgag
    11461 caaaatctcg cagatcatcc agctcatgtg gcttgaagaa gtctttggtc aaaaaccgct
    11521 gctcaaggtc aatgtcgcga ctctctacaa agcgtaacaa cataaccagg ttgttagccg
    11581 ccgccaacat cgttgaaaaa gcatcctctc tattgcggat ttgagttgtt agaaacagag
    11641 tggggtaata catgggaagc ccagaagcac gatccactac catgcaatag cgctcgccac
    11701 tatccatgac aaatgttgta actctgtagt tcttttccat gcactcacac cccataaaca
    11761 ttacacaatt acaaggtaca ctttatgtga aaaatcacca atttatacaa gacacttttt
    11821 ccgacgatag aaacaaaaaa cccctgaatt aacaggggcc tgcgtatgga ttctttacaa
    11881 ataactcaca agttaactct agaacggaat atcgtcatca aagtccatcg gcggctcgtt
    11941 agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc tgaactgatt
    12001 gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac caccgatatt
    12061 gccacctgca ggagcgccac caccctgacg accacccagc atctgcatgg tgccgccaac
    12121 gttcaccacg acttctgtgg tatagcgatc ctgaccggat tgatcggtcc atttgcgggt
    12181 acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc tggctacttc
    12241 tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgctctttca tctcgccggt
    12301 ggctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg cgccaccatt
    12361 tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa ccttgtttac
    12421 gcctctgctg gccatgttcg tgtctcctga aaaaaaatcg ttctgaataa gtgtaaacgc
    12481 gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa
    12541 acctggccag acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat
    12601 tcaggttaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg
    12661 caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca
    12721 cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg
    12781 ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc
    12841 gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggagaagc
    12901 cggacgtcga ccatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt
    12961 ctcataaccc gcgctctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtctgc
    13021 tgtacgctcg cgttggtgag ccgcgctgcc cggaccacga tgtaccgctg gcggcgcaaa
    13081 ccgtcagtca gatggtggat aacgtgttgt cccagccgga cggcaagcgt ctgatgctgc
    13141 tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa
    13201 gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga
    13261 aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaagtgcgtg
    13321 acgatcttac ccaacgtctt gccgagtcgt ttgaaaccgc gctggagctt tccggtggta
    13381 ccgcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc tccgccaact
    13441 ttgcctgccc gatttgcggt tacagtatgc gcgagctgga acctcgcctg ttttcattta
    13501 acaacccggc gggagcctgc ccgacctgtg acggacttgg cgtacagcaa tatttcgatc
    13561 ctgaccgcgt gatccagaat ccggaattat cgctggctgg cggtgcgatc cgtggctggg
    13621 atcgccgcaa cttctattat ttccagatgc tgaaatcgct ggcagatcac tataagttcg
    13681 acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt
    13741 ctggcaaaga aaatattgaa ttcaaataca tgaacgatcg tggcgatacc tccattcgtc
    13801 gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag acggaatcca
    13861 gcgcggtacg tgaagaatta gccaagttta tcagcaatcg tccgtgcgcc agctgcgaag
    13921 gaacccgtct gcgtcgggaa gcacgccacg tgtatgtcga gaatacgccg ctgcccgcca
    13981 tctccgacat gagcatcggt catgcgatgg aattcttcaa caatctcaaa ctcgcaggtc
    14041 agcgggcgaa gattgcagaa aaaatcctta aagagatcgg cgatcgtctg aaattcctcg
    14101 ttaacgtcgg cctgaattac ctgacacttt cccgctcagc agagacactt tccggcggtg
    14161 aagcccagcg tattcgtctg gcgagccaga ttggtgcagg cctggttggc gtgatgtacg
    14221 tgctggatga gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg ctgggtacgc
    14281 ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcacgac gaagacgcga
    14341 ttcgcgccgc tgatcatgtg atcgatatcg gtccgggtgc gggtgttcac ggcggtgaag
    14401 tcgtcgcgga aggtccgctg gaagcaataa tggcggtgcc tgagtcgttg accgggcagt
    14461 acatgagcgg taaacgcaag attgaggtgc cgaagaaacg cgttccggca aatccggaaa
    14521 aagtgctgaa actgacgggc gcacgcggca acaacctgaa agacgtgacg ctgacgctgc
    14581 cggtgggtct gtttacctgc attaccggag tttcaggttc cggtaaatca acgctgatta
    14641 acgacacact gttcccaatt gcccaacgcc agttgaatgg tgcgaccatc gccgaaccag
    14701 caccgtatcg cgatattcag gggctggagc atttcgataa agtgatcgat atcgaccaaa
    14761 gcccaattgg tcgtactccg cgttctaacc cggcgaccta taccggcgtg tttacgcctg
    14821 tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt
    14881 tcagctttaa cgttcgcggc ggacgctgcg aagcctgtca gggcgacggc gtgatcaaag
    14941 tggagatgca cttcctgccg gacatttacg taccgtgcga ccagtgcaaa ggcaaacgct
    15001 ataaccgtga aacgctggag attaagtaca aaggcaaaac catccacgaa gtgctggata
    15061 tgaccatcga agaggcgcgt gagttctttg atgcggtgcc agctctggca cgtaagctgc
    15121 aaacattgat ggacgttggt ctgacgtata ttcgtctggg tcagtccgca accacacttt
    15181 ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc
    15241 agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac
    15301 tgctcgacgt actgcataaa ctgcgcgatc agggcaatac cattgtggta attgagcaca
    15361 atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa ggcggcagtg
    15421 ggggcggcga aatcctcgtc tccggtacgc cagaaaccgt cgcagagtgc gaagcttcgc
    15481 atacggcgcg cttcctcaag ccgat
//
LOCUS       DAOFSV010000103        15419 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.103, whole genome shotgun sequence.
ACCESSION   DAOFSV010000103 DAOFSV010000000
VERSION     DAOFSV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.103"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            207..770
                     /gene="fimA"
                     /locus_tag="QBH27_003344"
     CDS             207..770
                     /gene="fimA"
                     /locus_tag="QBH27_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDQ7015623.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1132..1842
                     /locus_tag="QBH27_003345"
     CDS             1132..1842
                     /locus_tag="QBH27_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ7015624.1"
                     /translation="MQTTRTPYSVSFMATVLLLFLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLLGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSILTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPLIKATMQ"
     gene            1884..4535
                     /locus_tag="QBH27_003346"
     CDS             1884..4535
                     /locus_tag="QBH27_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ7015625.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            4549..5079
                     /locus_tag="QBH27_003347"
     CDS             4549..5079
                     /locus_tag="QBH27_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7015626.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHTGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            5092..5595
                     /locus_tag="QBH27_003348"
     CDS             5092..5595
                     /locus_tag="QBH27_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7015627.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYIWQ"
     gene            5655..6569
                     /locus_tag="QBH27_003349"
     CDS             5655..6569
                     /locus_tag="QBH27_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ7015628.1"
                     /translation="MGKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVILPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKSGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
     gene            6908..9187
                     /gene="ydeP"
                     /locus_tag="QBH27_003350"
     CDS             6908..9187
                     /gene="ydeP"
                     /locus_tag="QBH27_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDQ7015629.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMVHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            9435..9632
                     /gene="safA"
                     /locus_tag="QBH27_003351"
     CDS             9435..9632
                     /gene="safA"
                     /locus_tag="QBH27_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDQ7015630.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRMARDLTHFIINSLQD"
     gene            9707..10468
                     /gene="ydeO"
                     /locus_tag="QBH27_003352"
     CDS             9707..10468
                     /gene="ydeO"
                     /locus_tag="QBH27_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDQ7015631.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNTMNALAS"
     gene            10870..12552
                     /locus_tag="QBH27_003353"
     CDS             10870..12552
                     /locus_tag="QBH27_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDQ7015632.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            12604..13761
                     /locus_tag="QBH27_003354"
     CDS             12604..13761
                     /locus_tag="QBH27_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDQ7015633.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            13773..14684
                     /locus_tag="QBH27_003355"
                     /pseudo
     CDS             13773..14684
                     /locus_tag="QBH27_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            15046..>15419
                     /locus_tag="QBH27_003356"
     CDS             15046..>15419
                     /locus_tag="QBH27_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ7015634.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT         4526 a         3309 c         3329 g         4255 t
ORIGIN      
        1 caacaagtgc agcaataaaa ttgttgtgaa taggtgtcct tgtaatttaa agatattatc
       61 attcttcgtg aaatgtaata accatggaaa ctatattctc agggcaacat ccaatcaaga
      121 atgattcttg ttttataagc aataacagaa taacttaatt gttaactctc tttattcagc
      181 tacttaaata taaattttgg agaattatga aactcaaaca tgtcggtatt attgtcgttt
      241 ctgtgttggc gatgtcgtct gctgcggtaa gcgcagccga gggtgatgaa tcagtaacga
      301 ccactgttaa tggcggtgta attcatttta aaggtgaagt ggtaaatgcc gcttgtgcga
      361 ttgattcaga atcaatgaac caaacggttg agctgggtca ggttcgttct tctcgcctgg
      421 ctaaagcggg tgacctcagc tccgccgttg gcttcaatat caagctgaat gattgtgata
      481 ccaatgtttc cagtaatgca gctgttgcat tcctgggtac tactgtcacc agtaatgacg
      541 atacattagc gctgcagagt tcagcggcag gctctgccca aaatgtcggt atccaaattt
      601 tggaccgtac aggggaggta ttagtacttg atggtgctac ttttagcgct aaaactgact
      661 tgattgatgg cacgaatata ctgccgttcc aggctcgtta tattgctctc gggcagtcag
      721 tagctggtac tgcaaatgct gatgcgacct tcaaagttca atatctataa ttcactctta
      781 aggtgactat gcgtcatctg aaattgaaac agggtgtgtg tggcatggct acgcacaccc
      841 gagaaattca ccaatgttca tctcattatt aatgaaaaat ggtttgtatt ttggagcggt
      901 atgaaaaatc acataatatc taaacgatca ataaacaaca gtagttaaac ttgctgttat
      961 ttaaaatatt tacttggtag caaggttttc ctatcaaaag agaaccaaca tttattacaa
     1021 acgcattaag tctggaaacc cggcgactcg ccaatgtatt agcaatattt accgattgtc
     1081 tgatgtaacg gtttccccgc ttatcctctt aacacatgga gaatgcatca gatgcaaact
     1141 acgcgtaccc cctactctgt ttcctttatg gcgacagtgt tacttttatt tctctttgct
     1201 tgccatagca ccattgccaa cgcagctgtc gcacttggtg cgacgcgggt aatttatccg
     1261 gcaaaccaga aacaggtttt attgcctgtc acgaataatg atcctgccag tgtctatctc
     1321 attcagtcat ggatagaaaa tgcgggtgac cagaaagata cccagtttgt cattacccca
     1381 ccgctttttt ccatgcaagg taaaaaagag aatacgcttc ggataatcaa cgccactaat
     1441 catcagttac taggcgaccg tgaaagcctg ttctgggtta atgttaaagc tatccctgct
     1501 atggaaaaag accagaagaa tgagaataca ttacagctgg caattattag ccgcataaaa
     1561 atgttttatc gccccacgca tctggcaatg gcacctgaag aagcgcctgc aatgcttcgg
     1621 tttcgtcgca gcggtagcat actcactctt attaatccaa cgccctactt cattaccgta
     1681 acaaatatga aagcaggaaa tagcaatctc ccaaacacca tggtgccgcc aaaaggtgaa
     1741 gtatcggtgg atatcccgca tgctgtaacc ggggatatca gttttcagac aatcaatgat
     1801 tacggtgcat taactccgct tattaaggca accatgcagt aagccagtga cctaattaat
     1861 aataaactca atcgggatta aaaatgacag cctttcgtgc cgcatttaag gcatatcgta
     1921 tgcatcaggt gttaatactg cctcgctttg cacggcttac ctttgccctt ggcttagcaa
     1981 ctgcggtttt tcccgttgac gcagagtatt attttaatcc acggttttta agcaatgacc
     2041 ttgctgaatc tgtcgattta tctgccttta ctaagggacg cgaggcccca cctggtacat
     2101 atcgggtcga catttatttg aacgacgaat ttatggctag tcgggatatt acttttattg
     2161 cagatgataa taacgcagat ctcatcccat gcctgagtac agaccttctt gtcagtcttg
     2221 gcataaaaaa atcagcatta ttagacaaca aggaacattc ggcagataaa catgtgccgg
     2281 acaacagcgc atgcacacca cttcaggatc gtttggctga cgcatcatct gagtttgatg
     2341 ttggtcagca acatctgtca ctctccgttc cgcaaatata tgtcggcaga atggctcgcg
     2401 gctatgtttc tcctgatctg tgggaagaag gaataaatgc cgggctacta aactatagtt
     2461 ttaatggtaa ttctattaat aaccgtagta accataatgc aggaaaatcc aactatgcat
     2521 atttgaattt acagagtggc atcaacattg gtagttggcg actgcgcgat aactcaacgt
     2581 ggagttataa cagtgggagt agcaattcat ctgacagcaa taaatggcag catatcaata
     2641 cgtcggctga acgtgacatt attccgttac gctcacggtt aacagtgggt gatagttata
     2701 ccgatggcga tatttttgac agtgtgaact ttcgtggcct taaaataaat tcaacagaag
     2761 cgatgttgcc cgatagccaa catggtttcg ctccggtgat tcatggtatt gctcgtggca
     2821 ccgcacaagt gagtgtaaaa cagaatggat acgatgttta tcagactact gtcccacccg
     2881 gcccttttac tattgatgat atcaactctg cggccaatgg tggtgatctg caagtaacca
     2941 taaaagaggc agacggcagt attcagacat tatatgttcc ttattcgtct gttccggttc
     3001 tccaacgtgc tggatatacg cgttatgcac ttgccatggg ggaatatcgt agtgggaata
     3061 acctgcaaag ctcccccaag ttcgtacaag caagcttgat gcatggtctg aaaggaaact
     3121 ggacacctta tggcggaatg caaattgcag aagattatca ggccttcaac cttggtattg
     3181 gtaaagattt aggacttttt ggtgcctttt ctttcgatat cacgcaggcc aatacgacgc
     3241 ttgcagatga cacccgtcac agcggacaat cggttaaatc cgtctacagc aaatccttct
     3301 accagacggg aaccaatatc caggtcgcag gatatcgcta ttctacgcaa ggtttttata
     3361 acttatccga cagtgcatac agtcgaatga gtggttacac cgtcaagcct cctaccggag
     3421 acaccagtga gcagacacta tttattgatt attttaatct gttctacagt aagcgtggtc
     3481 aggaacaaat aagcatctct cagcagcttg gacattacgg tacgacattt ttcagtgcca
     3541 gtcgccaaag ttactggaac acgtcacgca gcgaccagca aatatcattt ggattaaatg
     3601 tgccgtttgg tgatattacg acttcgctga attacagcta ttccaataat atatggcaaa
     3661 acgatcggga tcatttactc gcttttacac ttaatgttcc ctttagtcat tggatgcgta
     3721 cagacagtca gtcggcattt cgtaattcaa acgccagtta cagtatgtca aacgatttga
     3781 aaggcggcat gaccaaccta tcaggggttt atggcactct gctgccggat aataacctga
     3841 attatagcgt tcaggtcggt aacacccaag ggggtaatac atcgtctggc accagtggtt
     3901 acagttctct taattatcgt ggagcttacg gtaatacgaa tgtcggttac agtcggagtg
     3961 gtgacagcag ccagatttat tacggaatga gtggtgggat tattgctcat gctgatggca
     4021 tcacctttgg acagccgctg ggcgacacaa tggttctggt taaggctcct ggcgctgata
     4081 atgtcaaaat agagaaccag accggaattc ataccgactg gcgtggctat gccatattac
     4141 catttgcgac agaatataga gaaaatcgtg tcgctcttaa cgcgaattcc cttgcagata
     4201 atgttgaact ggatgaaacc gtagtcactg tcatcccaac tcacggtgct attgccagag
     4261 caacatttaa tgcacaaatc ggcgggaaag tattaatgac gttgaagtac ggtaataaaa
     4321 gcgttccatt cggtgcaatt gtcactcacg gagagaataa aaatggcagc attgtcgcgg
     4381 aaaatggtca ggtttatctg actggacttc cacagtcagg gaaattacag gtttcatggg
     4441 gcaatgataa aaactcaaac tgtattgtcg attacaagct tcctgcagtc tctcctggaa
     4501 ccttgctgaa ccagcagaca gcaatctgtc gctaaggaac aatagattat gaaatacaat
     4561 aacgttattt tcctcggttt atgtctgggg ttaaccacct attctgcttt atccgcagat
     4621 agcgttatta aaattagcgg gcgcgttctc gattatggct gcacagtctc atcggattcg
     4681 cttaatttta ccgtagatct ccaaaaaaac agtgccagac aatttccaac gaccggtagc
     4741 acaagtcctg ccgtcccttt tcagattacg ttaagtgaat gcagcaaagg gacaacgggg
     4801 gtgcgggttg catttaacgg tattgaggat gcagaaaata atactctgtt gaaactggat
     4861 gaaggaagca atacggcctc cggtttgggt atagaaatac tggacggaaa tatgcgtccg
     4921 gtgaaattga atgaccttca taccgggatg cagtggatcc cattggtacc agaacagaac
     4981 aatattttgc cttactccgc tcgtttgaag tcaactcaga agtccgtcaa tccaggactg
     5041 gtgagcgctt cggcaacctt tacccttgaa tttcaataat cagagtcaga tatgaaaaga
     5101 cttcacaaga ggttcctgtt agctacgttt tgcacgttat ttacagcaac tctccaggcc
     5161 gccgatgtca ctatcactgt taatggtcgg gtagtcgcta aaccatgcac tattcaaacc
     5221 aaagaagcta acgttaatct cggggatctt tatacacgca atctgcaaca acctggttct
     5281 gcatctggct ggcacaatat tactttgtca ttaaccgatt gtccgattga aacaagtgca
     5341 gtgacggcaa tcgtgacagg ttcaactgac aatacgggtt attacaaaaa tgaaggtact
     5401 gccgaaaata ttcagataga gctaagggat gaccaggatg cgacgttaaa aaatggcgat
     5461 agcaaaacgg ttattgttga tgagatcact cgtaatgcac agtttccact taaggcaaga
     5521 gctatcacgg tgaatggaaa cgcaagccag ggaacgatcg aggcgctaat caatgtgatc
     5581 tatatctggc aataacatca atttgatttt atttgcctcc tttttaaatc aattttttaa
     5641 ccttaggata tatcatgggt aaaacattca gtataaaggt cctgttcggt atttatcttc
     5701 tattaatggc aggcaaagtt tttgccttta gttgtaatgt cgatggcggg tcaagtattg
     5761 gagcaggtac aacctctgtt tatgttaatc tcgatcctgt aatactaccg ggccagaatc
     5821 tggttgtaga cttgtctcag catataagtt gctggaatga ttacggcggc tggtacgaca
     5881 ctgatcatat aaacctggta caaggatcag ctttcgcagg ctccctacag tcatataaag
     5941 gatcactgta ctggaataat gtgacctacc ccttcccgtt gacgacaaat accaacgtat
     6001 tggatattgg tgataagaca ccgatgccat taccgttaaa actctatatt accccagtcg
     6061 gagcagccgg tggagttgtc ataaaatcag gggaagttat tgcccgtatt catatgtata
     6121 aaattgccac gttaggaagc ggaaatccac gtaattttac gtggaatatt atttcaaata
     6181 acagtgttgt tatgccaaca ggcggttgca ccgttgatag tcgtaatgtc accgtgaatc
     6241 tcccggactt tcctggaagc gcagaaatac cccttggtgt atattgctct agcgaacaaa
     6301 agctctcatt ttatctttcg ggtgccacca ctgacagtgc ccgacaagtg tttgctaaca
     6361 cagcaccaga tgcaacaaaa gcatcgggcg tgggtgtctc tctgatgcgt aatggaaaaa
     6421 cactggcaac aggagaaaat gtctccctgg ggacagtaaa taaaagcaaa gtacctctgg
     6481 gtttaagcgc aacctatgga caaacaggaa ataaggttgc agccggagcc gtccagtcag
     6541 tcattggtgt cacttttatc tatgaataat tgtgattttg acattagtct atgcgctcca
     6601 ttcacgaaaa ttgcgcggat aattattttg tgaaggctat tatcctacac ttgtaagaaa
     6661 atccgcgcac aattgaagcc agagtcttat tttttatatt gagaattatg ttaatttaaa
     6721 ccctatgaga gatgatgctt aacttatcgt atttatacta ctctattatt taacgataat
     6781 agagtaaaaa tagtaagaga gtagtcccct actcttatat atccatgttg acgataaacc
     6841 ctatgtatat attgtgcatc atcgctatta caaatcctaa taattcattt ccacacagga
     6901 taagcagatg aagaaaaaaa ttgaatccta ccagggagct gccggtggtt ggggtgctgt
     6961 taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg ttattgccat
     7021 gtttgacatg aataagccag agggctttga ctgtccggga tgtgcatggc cagatcctaa
     7081 gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct gggaagtcac
     7141 ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat tacttacctg
     7201 gggagatcac gagcttgagg ctgcgggacg tctcactcag cctttgaaat atgatgacgt
     7261 cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg gcgctcgcct
     7321 tcaaagctat agtgatccca atcaggttga attctatact tcggggcgca cttccaatga
     7381 agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact ttcccgactg
     7441 ctccaacatg tgccatgaac cgaccagtgt gggattggca gcgagtatcg gtgtgggtaa
     7501 agggaccgtg ttgctggaag actttgagaa atgcgattta gtcatttgca ttgggcataa
     7561 ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga aacggggagc
     7621 gaaaatgatc gctatcaacc ctctacagga acgtggcctg gagcgattta ccgcaccgca
     7681 aaacccgttt gaaatgctga cgaactctga gactcagtta gccagtgcct actataacgt
     7741 gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa ttgagcgcga
     7801 tgatgctgca agcgccgcag gtcggccctc attgctggat gacgaattta ttcaaacgca
     7861 taccgtcggc tttgacgagc tacgccgtga cgtgctcaat tccgagtgga aagatatcga
     7921 acgtatttct ggactaagtc agacacaaat cgccgaactt gctgatgcat atgccgctgc
     7981 cgaacgaacc attatctgtt acggaatggg gatcactcag cacgaacatg gtacccagaa
     8041 cgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc ctggtgcggg
     8101 tatctgccca ctacgaggtc actctaatgt acagggcgac cgaaccgtcg gtatcaccga
     8161 gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttca ccccacctca
     8221 tgcacctgga catgctgcaa tagccagcat gcaagctata tgtacggggc aagctcgagc
     8281 attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag cgagcgctgt
     8341 accgttaacg caattagatc tggcggtaca cgtagccact aagcttaacc gctctcatct
     8401 gctgaccgca cggcatagct atattctgcc ggttctggga cgtagcgaga ttgacatgca
     8461 aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatggttc atgcctcgcg
     8521 tggtgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg tcgcgggaat
     8581 cgcgcaggca gcactacccc agagcgtggt agcctgggag tatctggtgg aagattatga
     8641 tcgcattcgc aatgacattg aagctgtgct gccagagttt gccgactata accagcgcat
     8701 ccgtcatcct ggtggttttc acctgataaa cgcagctgct gaaaggcgct ggatgacgcc
     8761 gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatcctt cttcagcgtt
     8821 taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca cgacgattta
     8881 tggtatggat gatcgctatc gaggtgtatt cggtcaacga gatgtggtct ttatgagtgc
     8941 taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta ttgcactcac
     9001 gccagacggt aaacgtagct cacgccgcat ggatagatta aaagtggtca tttaccctat
     9061 ggctgaccgc tcactggtga cctatttccc agaatcgaat cacatgctaa cacttgataa
     9121 ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat tagaaccatc
     9181 aaattaattc tcctcctcat ttcttctgct gtcatccgca cagcagaaga attcctcatt
     9241 gactattatc gcgcaatttg ctcacatgga ttaaattaaa ctacacacta taagatataa
     9301 acctctgcct acagttgtaa gaaactccgc tcagtactga agtgccagtc ctatttcctc
     9361 ttttatccag cctgttatat taatcatact gattaacgat ttttaacgtt atccgctaaa
     9421 taaacacatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa agagacaact
     9481 ataaagaaat catgtctgta attgtggttg tcttgttact gacacttacg ttgatagcca
     9541 ttttttcggc aattgatcag ctgggcattt cagaaatggg tcgcatggca agagatctta
     9601 cacatttcat tatcaatagt ttgcaagact gaaaataaac agcaaattat acatgaaaaa
     9661 tgttaaaaaa gtatcgataa aaactttatt attttaagga gataaaatgt cgctcgtttg
     9721 ttctgttata tttattcatc atgccttcaa cgctaacatt ttagataaag attacgcctt
     9781 ctctgacggc gagatcctga tggtagataa cgctgttcgc acgcattttg aaccttatga
     9841 gcggcatttc aaagaaatcg gatttaatga aaataccatt aaaaaatatc tacaatgcac
     9901 taacatccag acagtgacga tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc
     9961 gactggattg cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa
    10021 tttttcagag cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat
    10081 tacactatta actaacggtg tgctatccgt ttccgggaaa gtaagaaata ttgtcaacat
    10141 gaagctggcg cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag
    10201 cctgttgaag aaaaaactaa agcaagagca aacgacattc tcacagattc ttttagatgc
    10261 cagaatgcag cacgcgaaaa atttgatacg cgtagaaggt tcagtcaata aaattgctga
    10321 acaatgcggt tatgccagta catcttattt tatttatgcg tttcgcaaac atttcggcaa
    10381 cagtccgaag agagtttcta aggagtaccg ttgtcaaagg cacacgggta tgaacacgga
    10441 caacacgatg aatgctttag ctagttgatt atttgctaac gagtagtcaa ccacacacgc
    10501 tgcgtaagaa ttaaatgggg cagccattcc ctgccccgcg ttgtttccag gcgatatatt
    10561 tcttgaaata aataagtgac agccatcaca tatttatgca ttttcataac ctgttgcatg
    10621 attatttatg atctcagctc ggcaatttgt cagtgaaata caataattta ttaaatatca
    10681 atagattagt tgtttatcgg cgagaaatta cttagtagaa cagaaagtaa tgtcaacgcg
    10741 ttatggactg ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt
    10801 cacttacatt cacttatttc tcttcgtaaa attactttgg aattaagtac aataagaaga
    10861 ggaacattta tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata
    10921 ctggcatctg gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca
    10981 accaaaacaa acgttgcgtt ctcagacttt acgccgactg aatacagtac caaaggaaag
    11041 ccaaatatta tcgtactgac catggatgat cttggttatg gacaacttcc ttttgataag
    11101 ggatcttttg acccaaaaac aatggaaaat cgtgaagttg tcgataccta caaaataggg
    11161 atagataaag ccattgaagc tgcgcaaaaa tcaacgccga cgctcctttc attaatggat
    11221 gaaggcgtac gttttactaa cggctatgtg gcgcacggtg tttccggccc ctcccgcgcc
    11281 gcaataatga ccggtcgagc gcccgcccgc tttggtgtct attccaatac tgatgctcag
    11341 gatggtattc cgctaacaga aactttcttg cctgaattat tccagaatca tggttattac
    11401 actgcagcag taggtaaatg gcacttatca aaaatcagta atgtgccggt gccggaagat
    11461 aaacaaacgc gtgactatca tgacaacttc actacctttt ctgcggaaga atggcaaccg
    11521 caaaaccgtg gctttgatta ctttatggga ttccatgctg caggaacggc atattacaac
    11581 tccccttcac tgttcaaaaa ccgtgaacgc gtccccgcaa aaggttatat cagcgatcag
    11641 ttaaccgatg aagcaattgg cgttgttgat cgtgccaaaa cacttgacca gccttttatg
    11701 ctttacctgg cttataatgc tccgcacctg ccaaatgata atcctgctcc ggagcaatat
    11761 cagaagcaat ttaataccgg tagtcaaaca gcagataatt actacgcttc cgtttattct
    11821 gttgatcagg gtgtaaaacg cattctcgaa caactgaaga aaaacggaca gtatgacaat
    11881 acaattattc tctttacctc cgataatggt gcagttatcg atggtcctct gccgctgaac
    11941 ggggcgcaaa aaggctataa gagtcagacc tatcctggcg gtactcacac cccaatgttt
    12001 atgtggtgga aaggaaaact tcaacccggt aattatgaca agctgatttc cgcaatggat
    12061 ttctacccga cagctcttga tgcagccgat atcagcattc caaaagacct taagctggat
    12121 ggcgtttcct tgctgccctg gttgcaaaat aagaaacaag gcgagccaca taaaaatctg
    12181 acctggataa catcttattc tcactggttt gatgaggaaa atattccatt ctgggataat
    12241 taccacaaat ttgtccgcca tcagtcagac gattacccgc ataaccccaa caccgaggac
    12301 ttaagccaat tctcttatac ggtgagaaat aacgattatt cgcttgtcta tacagtagaa
    12361 aataatcagt taggtctata caaactgacg gatctacagc aaaaagataa ccttgccgcc
    12421 gccaatccgc aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat cgataacagc
    12481 cagccaccgc ttagcgaggt aaatcaggag aagtttaaca atatcaagaa agcactaagc
    12541 gaagcgaaat aactaaacct ttatgcggcg gatttttccg ccgccttatt gagcgagata
    12601 gcgatgcacg ttacagccaa gccctccagt tttcaatgta atctcaaatg tgattactgt
    12661 ttttaccttg aaaaagagtc gcagtttact catgaaaaat ggatggatga cagcactctg
    12721 aaagagttca tcaaacaata tatcgcagcg tctggcaatc aggtctattt tacctggcaa
    12781 ggcggtgaac ccactctggc tggcctggat tttttccgta aagttattca ctatcaacaa
    12841 cgctatgcag gccaaaaacg tatttttaat gcattacaaa cgaatggcat tttattgaat
    12901 aatgaatggt gtgcctttct caaagaacat gagtttctgg ttggtatctc gatcgatggc
    12961 ccccaagaac tacatgaccg ttacagacgc agtaattcag gtaacggtac ttttgcaaaa
    13021 gtgatagcag ccatcgagcg tctgaaatca tatcaaatag agtttaatac gttaaccgtt
    13081 attaataaca ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc
    13141 agtaaacata tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt
    13201 ggtcatagtg agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc
    13261 aagtttatgt caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatc
    13321 cgtcagtttg aaagctttgt cagccgtttt ttggggaatg ggcataccag ttgtattttc
    13381 caggagtcct gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac
    13441 cattttgtct atccacagta caaaattgga aacattaata aatcggaact caaaacgatg
    13501 aacagtgtac aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgta
    13561 tataaaccta tctgcaatgg tggttgccct aagcatcgta ttactaaagt aaacaatgag
    13621 actgtttcct atttttgcga aggttataaa atcctttttt caaccatggt accttatatg
    13681 aacgccatgg tggagttagc taagaacaga gtaccgcttt accacattat ggatgttgca
    13741 aaacaaatgg agaataatta ataacgctcc cccacagcac gattaacgac aatatttggg
    13801 catcgctaca aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga
    13861 aattcgccca gcagatgtat gagacgcgct atgctgataa ccgcacgaaa ttaccgcaag
    13921 caaatcagat tgcacagttt actgccgcag atgcactggc tgccgatcgc cagttgtttt
    13981 catctccagc ggatatcacg tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg
    14041 cgttaattac gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta
    14101 aaccattaac tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaaccag
    14161 tggcacaggt ttcacagtgg aagcgttata attcccggac tcctgttaat ctggcgacgc
    14221 gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga tctacgcatt aatattcgtg
    14281 aacaggcatc cggggcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag
    14341 atatcagtca tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt
    14401 tagcgaatga agtgatggtt aagcgtctgg ccaaagggat cagtgagcaa gaactgaacg
    14461 aataccagca aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag
    14521 cgaacaccat tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg
    14581 agcaattgtt gaaacaaatg acggtagaga atgttaacac tgctgttaaa caatatcttt
    14641 ctcatccggt gaatacctat accggagtat tattgccaaa ataatcacag ccccgtcaac
    14701 acatcgttgg cggggatttc atcaatattc gctattttta tgtaataatt ttataaatgt
    14761 gttcaaagta aataaccgtg taccaataat gatattataa ggtctgattt ttacgtgata
    14821 attcaggaga cgatagaaaa cacataaaat aacagcataa aacaccttac ctccacacag
    14881 aattctctat tatgttgttt tttaataaaa atacattatt cacgcaatat ctcgcgataa
    14941 ataacattag gattttgtta tttaaaaccg agtcctttgc acttgcttac tttatcgata
    15001 aatcctactt ttttaatgcg atccaatcat tttaaggagt ttaaaatgga taagaagcaa
    15061 gtaacggatt taaggtcgga actactcgat tcacgttttg gtgcgaagtc tatttccact
    15121 atcgcagaat caaaacgttt tccgctgcac gaaatgcgtg atgatgtcgc attccagatt
    15181 atcaatgacg aattatatct tgatggcaac gctcgtcaga acctggccac tttctgccag
    15241 acctgggacg acgaaaatgt ccataagttg atggatttat ccattaacaa aaactggatc
    15301 gacaaagaag aatatccgca atctgcagca atcgatctgc gttgcgtaaa catggttgcc
    15361 gatctgtggc atgcgcctgc accgaaaaat ggtcaggccg ttggcaccaa caccattgg
//
LOCUS       DAOFSV010000104        15398 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.104, whole genome shotgun sequence.
ACCESSION   DAOFSV010000104 DAOFSV010000000
VERSION     DAOFSV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.104"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1190
                     /gene="alsA"
                     /locus_tag="QBH27_003357"
     CDS             <1..1190
                     /gene="alsA"
                     /locus_tag="QBH27_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDQ7015635.1"
                     /translation="IIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKT
                     LMLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTV
                     MKDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRD
                     RKKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPL
                     DAVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAEN
                     QRELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIY
                     KVMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWAL
                     PQE"
     gene            1169..2149
                     /gene="alsC"
                     /locus_tag="QBH27_003358"
     CDS             1169..2149
                     /gene="alsC"
                     /locus_tag="QBH27_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDQ7015636.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            2160..2855
                     /gene="alsE"
                     /locus_tag="QBH27_003359"
     CDS             2160..2855
                     /gene="alsE"
                     /locus_tag="QBH27_003359"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDQ7015637.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     RERKDLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            2839..3768
                     /gene="alsK"
                     /locus_tag="QBH27_003360"
     CDS             2839..3768
                     /gene="alsK"
                     /locus_tag="QBH27_003360"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDQ7015638.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            4041..6026
                     /gene="yjcS"
                     /locus_tag="QBH27_003361"
     CDS             4041..6026
                     /gene="yjcS"
                     /locus_tag="QBH27_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDQ7015639.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMNNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            6235..6510
                     /locus_tag="QBH27_003362"
     CDS             6235..6510
                     /locus_tag="QBH27_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015640.1"
                     /translation="MPTVLSRIAMQLKKIAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            6529..7560
                     /gene="mdtN"
                     /locus_tag="QBH27_003363"
     CDS             6529..7560
                     /gene="mdtN"
                     /locus_tag="QBH27_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDQ7015641.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVSDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            7560..9611
                     /gene="mdtO"
                     /locus_tag="QBH27_003364"
     CDS             7560..9611
                     /gene="mdtO"
                     /locus_tag="QBH27_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDQ7015642.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSIVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            9608..11074
                     /gene="mdtP"
                     /locus_tag="QBH27_003365"
     CDS             9608..11074
                     /gene="mdtP"
                     /locus_tag="QBH27_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDQ7015643.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLNGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAISAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            11272..13419
                     /locus_tag="QBH27_003366"
     CDS             11272..13419
                     /locus_tag="QBH27_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:11689..11691,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDQ7015644.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            13794..15362
                     /locus_tag="QBH27_003367"
     CDS             13794..15362
                     /locus_tag="QBH27_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ7015645.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
BASE COUNT         3779 a         3982 c         4021 g         3616 t
ORIGIN      
        1 atattatcga ctggcgagaa atgcgtgtcc gcgccgcaat gatgttatta cgcgtgggct
       61 tgaaagttga tttagatgag aaagtggcga acttatctat cagccacaag cagatgctgg
      121 aaattgccaa aacgctgatg ctcgacgcca aagtcatcat catggatgaa cccacctcct
      181 cactcaccaa taaagaggtg gactatctgt ttctgatcat gaatcagttg cgtaaggagg
      241 gtacggctat cgtctatatc tcgcataaat tggcggaaat tcgccgtatt tgcgaccgct
      301 atacggtgat gaaagacggc agcagcgttt gcagcggcat ggtgagcgat gtgtcaaatg
      361 acgatatcgt ccgtctgatg gtaggccgcg aactgcaaaa ccgttttaac gcgatgaagg
      421 agaatgtcag caaccttgcg cacgatacgg tttttgaggt gcgcaacgtc accagccgtg
      481 acagaaaaaa ggtccgggat atctcattta gcgtctgccg gggagaaata ttaggctttg
      541 ccggactggt cggttccgga cgtactgaac tgatgaactg cctgtttggc gttgataaac
      601 gcgctggcgg agaaatccgt cttaatggca aagatatctc tccacgttct cccctggatg
      661 ccgtgaaaaa agggatggct tacatcactg aaagccgccg ggataacggc tttttcccca
      721 acttttctat cgctcagaac atggctatca gccgcagtct gaaagacggc ggctataaag
      781 gcgcgatggg gttgtttcat gaagttgacg agcaacgtac cgctgaaaat caacgcgaac
      841 tgctggcgct gaaatgtcat tcggtaaacc agaacatcac tgaactctcc ggcggtaacc
      901 agcagaaagt cctgatctcc aaatggctgt gctgtcgccc ggaagtgatc attttcgatg
      961 aacctacccg tggcatcgac gttggcgcga aagccgaaat ttacaaagtg atgcgccaac
     1021 tggcggacga cggaaaagtc atcctgatgg tgtcatctga actacctgaa attatcaccg
     1081 tctgcgaccg catcgccgtg ttctgcgaag gacgactgac gcaaatcctg acgaatcgcg
     1141 atgacatgag cgaagaggag attatggcat gggctttacc acaagagtaa aaagcgaagc
     1201 gagcgagaag aaaccgttca actttgcgct gttctgggat aaatacggta ctttttttat
     1261 cctggcgatc atcgtcgcca tttttggttc gctgtcgtca gaatattttc tgaccaccaa
     1321 taatattacc cagatttttg tgcaaagctc cgtgacggta ctgatcggca tgggggaatt
     1381 cttcgctatc ctggtcgccg gtatcgacct ttcggttggc gcgattctgg cgctttccgg
     1441 tatggtgacc gctaaactga tgttggcagg tattgatccg tttctcgcgg cgctaattgg
     1501 cggtgtactg gttggcggcg cactgggggc gatcaacggc tgcctggtca actggacggg
     1561 actacacccg ttcattatca cccttggcac caacgctatt ttccgtggga tcacgctggt
     1621 gatctccgat gccaactcgg tatacggctt ctcatttgac ttcgtgaact tctttgccgc
     1681 cagcgtaatt gggatacctg tccccgttat cttctcgcta attgtcgcgc tcatcctttg
     1741 gtttctgaca acgcgtatgc ggctcgggcg caacatctac gcactgggcg gcaacaaaaa
     1801 ctcggcgttc tattccggga ttgacgtgaa attccacatc ctggtggtgt ttatcatctc
     1861 cggtgtttgt gcaggtctgg caggcgtcgt ctcaactgca cgactcggtg ccgcagaacc
     1921 gcttgccggt atgggttttg aaacctatgc cattgccagc gccatcattg gcggcaccag
     1981 tttcttcggc ggcaaggggc gcattttctc tgtggtgatt ggcgggttga tcatcggcac
     2041 catcaacaac ggtctgaata ttttgcaggt acaaacctat taccaactgg tggtgatggg
     2101 cggattaatt atcgcggctg tcgcccttga ccgtcttatc agtaagtaag gaattgaaca
     2161 tgaaaatctc cccctcgtta atgtgtatgg atctgctgaa atttaaagaa cagatcgaat
     2221 ttatcgacag ccatgccgat tactttcaca tcgatatcat ggacggccac tttgtgccga
     2281 atctgacgct ctcaccgttc ttcgtaagcc aggtaaaaaa actggcgagt aaaccgctcg
     2341 actgccatct gatggtaacg cgtccgcagg attacattgc tcaactggca cgtgcgggag
     2401 ctgatttcat cactttgcat ccggaaacta tcaacggtca ggcattccgc ctgattgatg
     2461 aaatccgccg tcatggcatg aaagtggggc tgattcttaa tccggaaacg ccggttgagg
     2521 ccatgaaata ctatatccat aaggctgata aaattacggt catgactgtc gatcccggtt
     2581 ttgccggaca acccttcatt cctgaaatgc tggataaagt tgccgaactg aaggcatggc
     2641 gtgaacgaaa agatctggag tacgaaattg aggtggacgg ttcctgcaac caggcaactt
     2701 acgaaaaact aatggcggca ggggcggatg tctttatcgt cggcacttcc ggcctgttta
     2761 atcatgcgga aaatatcgac gaagcatgga gaattatgac cgcgcagatt ctggctgcaa
     2821 aaagcgaggt acagcctcat gcaaaaacag cataacgtcg tagcgggcgt ggatatgggg
     2881 gcaacgcata tccgcttttg tctgcggaca gcagaaggtg aaacgctaca ctgcgaaaaa
     2941 aagcggaccg cagaagttat tgctcccgac ctggtgtcgg gtatcggcga aatgattgac
     3001 gagcaactca ggcgctttaa cgctcgctgt catggtctgg tgatgggatt tccggcgctg
     3061 gtcagtaaag ataaacgcac cattatttct acgcctaacc tgccgttaac agcggcagat
     3121 ttatatgatc tcgccgataa gctcgaaaat acgctgaatt gtccggttga gttttcccgc
     3181 gacgttaacc tgcaactttc ctgggacgta gtagaaaacc gccttacgca acaactggtt
     3241 ctggcggcct atctcggtac ggggatgggg ttcgcagtgt ggatgaacgg tgcgccgtgg
     3301 acgggtgcac acggtgtggc aggcgaactg ggccatatcc ccctgggaga tatgacccaa
     3361 cactgcgcgt gtggcaattc tgggtgcctg gaaaccaatt gctctggaat ggcgctaaga
     3421 cgctggtacg aacaacagcc ccgaaattac ccattgagcg atcttttcgt ccatgcggaa
     3481 aacgcccctt tcgtccagag tctgcttgaa aacgcggcac gagccattgc caccagcatt
     3541 aatctgttcg atcccgatgc ggtgattctg ggcggtggcg tgatggatat gcccgccttc
     3601 ccacgcgaga ctctcattgc catgacccaa aagtacctgc gccgtccact gccgtatcag
     3661 gtcgtgcgct ttattgccgc ctcatcttct gactttaatg gcgctcaggg tgcagcaata
     3721 ttggcgcatc aacgtttttt gccacagtcc tgtgctaaag tcccatgagt aaagcgttgg
     3781 cacagtagcg caatgtcccg ctgtgccaac gacaggtaca atatatataa taattacata
     3841 aaacagccca tattattccc agcaatgata gttctactgt aattttccct cttcagcaca
     3901 aatgcctaat aaaatggctc gttccgctca cacttccact tattaaagtc gcaaattccc
     3961 aattatgata acgccagctt taaatgacgt attttgcatg gattacatat ttacgacata
     4021 aatgataaat ggagatttat atgaataact ctcggttatt ccgtttgagc aggattgtta
     4081 ttgcgttcac tgccgccagc ggcatgatga taaataccgc ttacgcaaca gatgaggcga
     4141 aagccgccac tcaatatacc caacaggtta atcagaatta cgccaaatca ttaccgttta
     4201 gcgatcgcca ggattttgac gatgctcagc gtggatttat cgctccgctg ctggatgaag
     4261 gtattctgcg tgatgcgaac ggtaaaattt actaccgcgc gaatgattac aaatttgata
     4321 ttaatgccgc tgcgccggaa accgtaaacc ccagcctgtg gcgtcagtcg cagatcaacg
     4381 gcatttcagg cctgttcaaa gtcaccgata aaatgtatca ggtgcgcggc caggatatct
     4441 ctaacattac gttcgttgag ggcgagaaag gcattattgt tattgaccca ctggtgacgc
     4501 cacctgccgc aaaagccgca cttgaccttt acttccagca tcgtccgcaa aaaccgattg
     4561 ttgccgttat ctacactcac agccacgccg accactacgg cggcgtgaaa ggtattatct
     4621 ctgaagccga tgttaaatcc ggcaaagttc aggtgattgc tccagctggc tttatggacg
     4681 aagccatcag cgaaaacgtg ctggcgggta acatcatgag ccgccgtgcg ctctactctt
     4741 acggtctgtt actgccgcac aacgcgcaag gcaacgtggg caacggcctc ggcgtgacgc
     4801 tggcaacggg cgacccgagc attattgcac cgactaaaac tatcgtcaga actggcgaga
     4861 agatgattat cgacggcctg gagtttgact tcctgatgac cccaggtagc gaagcgccag
     4921 cggaaatgca cttctatatt ccggccctga aagccctgtg taccgccgag aatgccacgc
     4981 ataccctgca caacttctac accctgcgcg gcgcgaaaac ccgcgacacc agcaagtgga
     5041 ccgagtatct gaacgaaaca ttggatatgt ggggtaacga cgcggaagta ctgtttatgc
     5101 cgcacacctg gccggtctgg ggcaataagc atatcaatga ttatattggt aaatatcgcg
     5161 atactatcaa gtacattcac gaccagaccc tgcacctggc gaaccagggc tacaccatga
     5221 atgaaatcgg cgacatgatt aagctgccgc ctgcgcttgc caataactgg gccagccgcg
     5281 gctattacgg ttctgtcagc cacaacgccc gcgcggtata taacttctat cttggctatt
     5341 acgacggtaa cccggctaac ctgcatccgt atggtcaggt ggagatgggt aaacgttacg
     5401 tgcaggcgct gggcggttct gcccgcgtca tcaacctggc gcaagaagcg aacaagcaag
     5461 gtgattatcg ctggtcggca gaactgctga aacaggtgat tgccgccaac ccgggtgacc
     5521 aggtcgcgaa gaatctgcaa gcgaataact ttgaacagct gggctatcag gccgagtccg
     5581 ccacatggcg cggtttctac ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt
     5641 tcagccacgg caccaccggt tccccggaca ccattcgtgg gatgtcggtc gaaatgctgt
     5701 tcgactttat gtccgttcgc ctcgatagcg cgaaagccgc gggtaaaaat atcagcctga
     5761 acttcaatat gaacaacggc gataacctca acctgacgct gaacgatagc gtgcttaact
     5821 accgtaaaac actgcaaccg caagctgacg cctctttcta catcagccgt gaagatctgc
     5881 acgccgtgct gaccggacag gcaaaaatgg ctgatctggt gaaagcgaag aaagccaaaa
     5941 ttattggcaa tggtgcgaaa ctggaagaaa ttatcgcctg tctggataat ttcgatttgt
     6001 gggtgaatat cgtaacccca aattaatatc actcgctttt gggggcgtaa acgctcccga
     6061 aagtatttat attaaaaata tatacagaaa attaatacat aatatatata agttatgaat
     6121 gcggtggctg aataaagcta tttttattca tcgagaaaat agccatactg acgccttcaa
     6181 tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt taatatgcca
     6241 acagttctct ctcgcatagc gatgcaactc aaaaaaatag cctggataat tcccgtcttc
     6301 atggtttcgg gatgctcatt atctccggca atcccggtga tcggcgctta ttatcccggc
     6361 tggtttttct gcgccattgc cagccttatt ttgacgctca tcacgaggcg aattattcag
     6421 cggacaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
     6481 gccatgctgt tctggctggc atttttctaa tcaatttgag acgcgctcat ggaaagtacg
     6541 ccgaaaaaag ctcctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
     6601 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
     6661 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
     6721 agcgacaacc aggcggtgaa acagggcgat ttgctgttcc gcattgaccc gcgcccgtac
     6781 gaagccaatc tggcgaaagc tgaagcctcc ctcgcggcgc tggataagca aattatgctc
     6841 acccagcgta gcgttgacgc gcaacagttt ggtgccgact cggttaatgc cacggtagaa
     6901 aaagcccgtg ccgccgcgaa acaagccagc gatacgttac gccgtaccga accgctactg
     6961 cgagaaggtt ttgtttctgc ggaagaggtt gaccgtgcaa gaaccgccca gcgtgcggca
     7021 gaagcggacc ttaatgccgt attgttgcag gcgcagtcag ccgccagtgc cgtcagcggc
     7081 gtcgatgcgc tggtcgccca acgtgcggcg gtcgaagcgg atattgccct gaccaaactg
     7141 catctggaaa tggcgaccgt tcgcgcgccg tttgatggcc gggtcatttc cctcaaaacc
     7201 tccgtcgggc aatttgcttc tgccatgcgc cctattttca ccctaatcga cactcgtcac
     7261 tggtacgtga tcgccaattt ccgcgaaact gatctgaaaa atattcgctc aggtacaccc
     7321 gcaacgattc gcctgatgag cgacagcggc aaaaccttcg agggtaaagt ggattcgatt
     7381 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc gaaagtgtcg
     7441 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggttgataaa
     7501 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     7561 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     7621 agttaagcga gcggcggcca ggtcgtgtgc cgcagaccgt gcaactctgg gtaggctgcc
     7681 tactggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     7741 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     7801 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     7861 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     7921 gcacccatcg gctggggctg gtctttttcg ccgtcgccat tgtcgctatt tacgggcaaa
     7981 ccttccccgc catgctcgac tatccggaag tggtcgtgcg cttaaccctg tggtgtatcg
     8041 ttgtcggcct ctacccgacg ttattgatga cgttaatcgg cgtgctgtgg tttcccagcc
     8101 gcgctatcac gcagatgcat caggcgctta atgatcggct tgatgatgcc attagccacc
     8161 tgacggacag cctcgcaccg ctacccgaaa cgcggattga aagagaggcg ctggcgctgc
     8221 aaaaactcaa tgtcttttgc ctcgcggacg atgccaactg gcgaactcag agcgcatggt
     8281 ggcaaagctg cgtggcaacg gtaacctaca tttactcgac gctgaatcgc tacgatccca
     8341 cctcttttgc tgattctcag gcaattattg aattccgaca aaaattagcc tcagaaatca
     8401 acaaactgca gcatgccatt gccgaaggtc agtgctggca aagcgactgg cggatcactg
     8461 aaagtgaagc gatggcggca cgggaatgta acctggagaa tatctgccag acattgttgc
     8521 aactgggtca gatggacccg aatacgccgc caacgcccgc caccaaaccg ccatcaatag
     8581 tcgccgacgc ttttaccaat ccagactata tgcgctacgc ggtaaaaacg ctgctcgcct
     8641 gtttgatctg ttacaccttc tacagcggcg tggactggga aggcattcac acctgtatgc
     8701 tgacctgcgt gatcgtcgct aatccaaatg tcggttcgtc gtaccagaag atggtgctgc
     8761 gttttggcgg ggccttttgc ggcgcgattc tggcgctgtt attcacgcta ctggtcatgc
     8821 cctggctgga caatattgtc gaattgctgt ttgtgctggc accgattttc ctgttgggcg
     8881 catggattgc caccagctct gaacgctctt cttatatcgg cacacagatg gtggtcacct
     8941 tcgcgctcgc cacgctcgaa aacgtttttg gcccggtgta cgacctggtg gaaattcgcg
     9001 atcgcgctct gggtatcatc attggtaccg tggtgtcggc ggtgatttac acctttgtct
     9061 ggcctgaaag cgaagcgcgc acgctgccgc aaaaactggc tggcgcgctg ggtatgttga
     9121 gtaaagtaat gcggatccca cgccagcagg aagtcacggc tctgcgcact tatctgcaaa
     9181 ttcgcatagg tctgcatgcg gcgtttaatg cctgtgaaga gatgtgccaa cgcgtggtgc
     9241 tggagcgtca actggacagc gaagaacgcg ccttactgat tgaacgttcg caaacggtta
     9301 ttcgtcaggg ccgcgatatt cttcacgcct gggatgccac ctggaactcg gcgcaggcgc
     9361 tggataacgc actacagccg gacagagcag gtcagtttgc cgacgcactg gagaaatacg
     9421 ccgctggtct ggcaaccgca ctcagccgtt ctcctcaaat aacgcttgaa gaaacacccg
     9481 cctcgcaggc catcctgccc accttattaa aacaggagca acacgtctgc cagcttttcg
     9541 cccgcttgcc agactggaca gccccggcat taacgcccgc caaggaacag gcacaaggag
     9601 ccacacaatg atcaatcgtc aactttcacg tctgctgttg tgcagcattc tcggcagcac
     9661 gacgctgatt tccggctgtg ccctggtacg taaggattct gcgcctcatc aacagctcaa
     9721 accggaacaa atcaaactgg ccgatgatat tcatcttgcc agctccggct ggccgcaggc
     9781 gcagtggtgg aaacaactca atgacccgca gctggatgcg ctgatccaac ggactctaaa
     9841 tggttcacac accctcgccg aagcgaaact gcgggaagaa aaagcgcagt cccaggccga
     9901 tttgttagat gccggttcac aattacaggt cgcagcgtta gggatgctca accgccaacg
     9961 cgtctcggcg aacggctttt taagccctta tgcgatggat gcgccagcac tgggtatgga
    10021 cgggccgtac tatacggaag ccacagtcgg tttgtttgcc ggactggatc tcgatttgtg
    10081 gggtgtgcat cgctcagcgg ttgctgccgc cattagcgcg cataatgccg cgctggcaga
    10141 aaccgcagca gtagagctat cgctgaccac gggcgtagcg cagctttatt acagtatgca
    10201 ggccagctat cagatgctcg atctgttaga acaaactcgc gatgtgattg attacgcggt
    10261 gaaagcgcac cagagtaaag tggcgcacgg tctggaagcg caagtgcctt tccacggcgc
    10321 gcgggcacag attctggcgg tcgataaaca aattgctgcc gtcaaagggc aaatcaccga
    10381 aacgcgagaa tctctgcgtg cattgattgg cgcgggagcc agcgatatgc cggagatcaa
    10441 accggtggca ttaccgcaag tccagaccgg tattccggcg acactctctt atgagttgct
    10501 cgccagacgc ccggatctgc aagccatgcg ctggtatgtt caggcgtcat tagatcaggt
    10561 ggattccgcg cgggcgctgt tctatccgag ctttgatatc aaagcgtttt tcggcctgga
    10621 ttctatccac ctggatacct tattcaaaaa aaccagtcgc cagttcaact ttatcccagg
    10681 tctgaaattg ccgctgtttg acggtggacg gttgaatgcc aatctcgaag gcactcgcgc
    10741 cgccagcaac atgatgattg aacgttacaa ccagtcagta ctgaacgcgg tgcgtgacgt
    10801 tgccgtcaac ggcacacgtc tgcaaacgct caacgacgag cgagagatgc aggctgaacg
    10861 cgtagaagca acgcgcttca cccagcgcgc agccgaggcc gcctaccaac gcggcttaac
    10921 cagccgctta caggccaccg aagcccggtt gccagtgctt gccgaagaga tgtcattact
    10981 gatgctggac agccgccggg tgatccaaag cattcagttg atgaaatcgc tgggcggcgg
    11041 gtatcaggcg gctcccgtcg tcgagaaaaa ataaaatgtc taccgcgtga tggctgtcac
    11101 gcggtatttc gtttcgtcac gtcaaaactg acgacagcct gtttttcgtc agagttttga
    11161 ataaatagtg cccgtaatat cagggaatga ccccaaataa aatgtggcat aaaagatgca
    11221 tactgtagtc gagagcgcgt atgcgtgatt tgattaactg gagcgagacc gatgaaaaaa
    11281 gtcgtcacgg tttgccccta ttgcgcatca ggttgcaaaa tcaacctggt cgtcgataac
    11341 ggcaaaatcg tccgggcgga ggcagcgcag gggaaaacca accagggtac cctgtgtctg
    11401 aagggttatt atggctggga cttcattaac gatacccaga tcctgacccc gcgcctgaaa
    11461 acccccatga tccgtcgcca gcgtggcggc aaactcgaac ctgtttcctg ggatgaggca
    11521 ctgaattacg ttgccgagcg cctgagcgcc atcaaagaga agtacggtcc ggatgccatc
    11581 cagacgaccg gctcctcgcg cggtacgggt aacgaaacca actatgtaat gcaaaaattt
    11641 gcgcgcgccg ttattggtac caataacgtt gactgctgcg ctcgtgtctg acacggccca
    11701 tcggttgcag gtctgcacca atcggtcggt aatggcgcaa tgagcaatgc tattaacgaa
    11761 attgataata ccgatttagt gttcgttttc gggtataacc cggcggattc ccacccaatc
    11821 gtggcgaatc acgtaattaa cgctaaacgt aacggggcga aaattatcgt ctgcgatccg
    11881 cgcaaaattg aaaccgcgcg cattgctgac atgcacattg cactgaaaaa cggctcgaac
    11941 atcgcgctgt tgaatgcgat gggccatgtc attattgaag aaaatctgta cgacaaagcg
    12001 ttcgtcgctt cccgtacaga aggctttgaa gagtatcgta aaatcgttga aggctacacg
    12061 ccggagtcgg ttgaagatat caccggcgtc agcgccagtg agattcgtca ggcggcgcgg
    12121 atgtatgccc aggcagaaag cgccgccatc ctgtggggca tgggggtaac ccagttctat
    12181 cagggcgtgg aaaccgtgcg ttctctgacc agcctcgcga tgctgaccgg taacctcggt
    12241 aagccacatg cgggtgtaaa cccggttcgt ggtcagaaca acgtgcaggg tgcctgcgat
    12301 atgggcgcgc tgccggatac gtatccgggc tatcagtacg tgaaagatcc ggctaaccgc
    12361 gagaaatttg ccaaagcctg gggcgtagag agcctgccag cgcataccgg ttatcgtatc
    12421 agcgagctgc cgcaccgcgt ggcgcatggt gaagtgagag cggcgtacat tatgggcgaa
    12481 gatccgctgc aaactgacgc ggaactgtcg gcagtacgta aagcctttga agatctggaa
    12541 ctggttatcg ttcaggacat ctttatgacc aaaaccgcgt cggcggcgga tgtcatttta
    12601 ccgtcaacgt cgtggggcga gcatgaaggc gtgtttactg cggctgaccg tggcttccag
    12661 cgtttcttca aggcggttga gccgaagtgg gatctgaaaa cggactggca aatcatcagt
    12721 gaaattgcca ctcgtatggg ttatccgatg cactacaaca acacccagga gatctgggat
    12781 gagttgcgtc atctgtgccc ggatttctac ggtgcgactt acgagaaaat gggcgaactg
    12841 ggcttcattc agtggccttg ccgcgatact tcagatgccg atcaggggac ttcttatctg
    12901 tttaaagaga agtttgatac cccgaacggt ctggcgcagt tcttcacctg cgactgggta
    12961 gcgccaatcg acaaactcac cgacgaatac ccgatggtac tgtcaacggt gcgtgaagtt
    13021 ggtcactact cttgccgttc gatgaccggt aactgtgcgg cactggcggc gctggctgat
    13081 gaacctggct acgcacaaat caataccgaa gacgccaaac gtctgggtat tgaagatgag
    13141 gcattggttt gggtgcactc gcgtaaaggc aaaattatca cccgtgcgca ggtcagcgat
    13201 cgtccgaaca aaggggcgat ttacatgacc taccagtggt ggattggtgc ctgtaacgag
    13261 ctggtgaccg aaaacttaag cccgattacg aaaacgccgg aatacaaata ctgcgccgtg
    13321 cgcgtcgagc cgatcgccga tcagcgcgcc gccgagcagt acgtgattga cgagtacaac
    13381 aagttgaaaa ctcgcctgcg cgaagcggca ctggcgtaat accgcttaaa ctcacgttaa
    13441 aagcggtgat attacccaac gcctttgtat caatccggaa acgactctct gtttccggat
    13501 ttttttatac atacctgcgc agtcaaacca gcctatcctg gttattagcg acgtcttctc
    13561 ggttgttctt ctattccagt cacgattgca aatgagaatt atctttactc aattctcatc
    13621 gcggcctaac gtaataatac tttacatttg atgaaccaaa tttcggtaag gtgagaaatc
    13681 atcgaccgtt taactcgttc tattaacggg atattttggc gcgattaatg caacaaaacc
    13741 ccgtctgata gaatattaat aacctcaaag taatttcagg gattgggaag catatgatta
    13801 aaaaactatt acctttactg gttttaagca caatttccgc cacaaccgtt gccgctacac
    13861 cgcctaatac gttggttgta gcccaggggc tggatgatat tgtgagcctg gacccggcag
    13921 aagctaatga actctcaagt attcagactg taccaagtct gtatcaacgc gtagtgcaac
    13981 cggaccgtaa tgatccgacg aaaattactc cgattctggc ggaaagttgg caggctgacc
    14041 ccgcagccaa aacgctaacc ataaaactga aaagcgatgc gaaatttgcc tctggtaatc
    14101 cggtacgccc ggaagatgtg atttactctt atgtccgggc tgtcacactg aataaatccg
    14161 gtgcgtttat tctcaatgtg ctgggatggc agccggaaaa tatcgccagc cagttgaaga
    14221 aaatcgatga ccataccgtc caggtgcaat ggagtgccga cgtcagcccg gctctggcac
    14281 tgaatattct ctccacacca atcgcctcaa ttgttgacga gaagctggtt gcgccaaacg
    14341 ccaaaaataa cgactttggt aatgaatggc tgaagatgca ttccgctggc agcggcgcgt
    14401 ttaaaatgcg tacttaccaa ccgcatcagg ccatcgtgat ggaagccaac gccagctcac
    14461 caaccggcgc cccgaaactg aaaagcgtca tcatcaaaaa tgttcccgat ccggcaacac
    14521 gtcgcctgct gatccaacaa ggtgatgctg atatggctcg tgatctggga gccgatcaaa
    14581 tcgatgcatt acagggtaaa cctggcgtaa aagtgatgag cattgcctcc gccgaacaga
    14641 actacctggc cttcaatacc ggtaacaagg ataacccgct gatgagtaat cctgcgttct
    14701 gggaggccgc tcgctggttg gtcgattacg acggcatcac caaaaatctc ttaaaaggac
    14761 aatattttac ccatcagagt ttcctgcccg tgggctttcc tggagcactg gaagaaacac
    14821 cgtttacctt taatccagcc aaagcaaaag agatcctcgc taaagccgga atcaaagacc
    14881 cgcacttcac gcttgatgtc gaaaataaac cgccatttat taccatcgcg cagtcaattc
    14941 aggccagctt tgctcagggc ggtgtaaaag ttgatttgct gccagccgca gggagtcagg
    15001 tctactctcg cgttcgcgcc caccagcatc agggggccat tcgtatgtgg ttgcctgatt
    15061 attttgacgc acattccaac gccagctctt tcgcctataa cgatggcaaa tccagcaccg
    15121 ttgccgggtt aaacggctgg aaaatcccgg aactgaacaa agaaacgctg gcggccatcg
    15181 ctgaacctga tagcactaaa cgtcttgatt tgtataagaa aatgcaacaa gaactacaac
    15241 gtagttcccc gtatgtcttt attgatcaag gcaaaacgca aattgtcatg cgtgataatg
    15301 tgcaggggta tcaacaaggg ttgaatgccg atatggtctg gtttgatcag gtaaccaaat
    15361 aaccctgtat gcaactcgcg tccagccatg ttaccggg
//
LOCUS       DAOFSV010000105        15350 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.105, whole genome shotgun sequence.
ACCESSION   DAOFSV010000105 DAOFSV010000000
VERSION     DAOFSV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.105"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..100)
                     /locus_tag="QBH27_003368"
     CDS             complement(<1..100)
                     /locus_tag="QBH27_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDQ7015646.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQT"
     gene            complement(356..919)
                     /gene="pqiC"
                     /locus_tag="QBH27_003369"
     CDS             complement(356..919)
                     /gene="pqiC"
                     /locus_tag="QBH27_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDQ7015647.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLARVWSQEAASIAQEIKRLP"
     gene            complement(916..2556)
                     /gene="pqiB"
                     /locus_tag="QBH27_003370"
     CDS             complement(916..2556)
                     /gene="pqiB"
                     /locus_tag="QBH27_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDQ7015648.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(2561..3814)
                     /gene="pqiA"
                     /locus_tag="QBH27_003371"
     CDS             complement(2561..3814)
                     /gene="pqiA"
                     /locus_tag="QBH27_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDQ7015649.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(3944..5851)
                     /locus_tag="QBH27_003372"
     CDS             complement(3944..5851)
                     /locus_tag="QBH27_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015650.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(5863..7971)
                     /gene="rlmKL"
                     /locus_tag="QBH27_003373"
     CDS             complement(5863..7971)
                     /gene="rlmKL"
                     /locus_tag="QBH27_003373"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDQ7015651.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETANIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            8069..9178
                     /gene="ycbX"
                     /locus_tag="QBH27_003374"
     CDS             8069..9178
                     /gene="ycbX"
                     /locus_tag="QBH27_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDQ7015652.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRALQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPADEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSATGDDGTILCCSCVPKTALKLAR"
     gene            complement(9175..9717)
                     /gene="zapC"
                     /locus_tag="QBH27_003375"
     CDS             complement(9175..9717)
                     /gene="zapC"
                     /locus_tag="QBH27_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDQ7015653.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(9891..10901)
                     /gene="pyrD"
                     /locus_tag="QBH27_003376"
     CDS             complement(9891..10901)
                     /gene="pyrD"
                     /locus_tag="QBH27_003376"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDQ7015654.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11138..11713
                     /gene="ssuE"
                     /locus_tag="QBH27_003377"
     CDS             11138..11713
                     /gene="ssuE"
                     /locus_tag="QBH27_003377"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDQ7015655.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            11706..12665
                     /gene="ssuA"
                     /locus_tag="QBH27_003378"
     CDS             11706..12665
                     /gene="ssuA"
                     /locus_tag="QBH27_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDQ7015656.1"
                     /translation="MRKIIKLVLAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            12662..13807
                     /gene="ssuD"
                     /locus_tag="QBH27_003379"
     CDS             12662..13807
                     /gene="ssuD"
                     /locus_tag="QBH27_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDQ7015657.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPKLVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQASFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            13819..14610
                     /gene="ssuC"
                     /locus_tag="QBH27_003380"
     CDS             13819..14610
                     /gene="ssuC"
                     /locus_tag="QBH27_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDQ7015658.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRELVEMARS
                     YGLSGMPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            14607..>15350
                     /gene="ssuB"
                     /locus_tag="QBH27_003381"
     CDS             14607..>15350
                     /gene="ssuB"
                     /locus_tag="QBH27_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ7015659.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFE"
BASE COUNT         3634 a         4110 c         3826 g         3780 t
ORIGIN      
        1 gcgtctgata gggacacatt tcttttgagc gtccggcgat gccggcctga taaccacgtt
       61 gatgtgcccg ttccaggcga tctcgttttt gtctcttcat gcctcgtttc cctcatactg
      121 gtttctggtg gaaaagaaaa cagtgattac taaatatgca accacagtta agcaataccg
      181 cgaatcataa ctgccgtcaa tgcgcaaaat tcacgccact gtcatatttg tgagctacac
      241 gggcatgtcg gttgtacaaa aaatgtgaag aggaacaact ttttccgcac gttagaaaca
      301 aaaaaaccgc ctcaggcaac ataccggaga cggttatatt tacaaatctt tatgattaag
      361 gtagacgctt tatctcttgc gcaatagaag cggcttcctg actccagaca cgggccagca
      421 ctttaaccat ctcatcgtaa ccatcctgag tttgcactcc ttccagacgg aacggacgtt
      481 tgatcagttg tccctggtgg ttcaacagcc actcaccact gacaatgacc ttgccatcat
      541 agcgaccgtt aaactccgtg acggtaacat tgagcgtgtc ctgggcgctt cccagaggct
      601 gggaggcaac cacccagccg ggcagttgcg tactcaggtt ggcaaccagg gtgttgcgca
      661 actgttgatc caacgggctg gcccacaagt tgttgttggc aatcacatac ttcacatcac
      721 tggtttgata aaccacacca ttccccgcca gatagtcagg aacagcgacc tgctcaaccc
      781 ataacaggcg attaccctgg ctggcggtgc tttgtgtacc gctctgcacc acaggtaact
      841 ggtaatagtt tttattaatt tcaccggagc tgcatccggc cagccacagt gccgcaatcg
      901 tcactagcca ctttttcatt gtttcgccct cttcggctct ggatcttttt tgtccttcgc
      961 ttcaaatacc agcgcgttac tcttctcgtt gagcgttttc agcaccggtt gcagttctcg
     1021 caacacctga tcaaggcgct gcatatccgc caccatcttg ttgtaggctg cggaaccagg
     1081 ctggaagccc tgcatactgc gattcaattc acgcaacgtt gactgcatat ccgtcggtaa
     1141 ctgctgcatc gactggctgg cgaggatctt gttcatgcta tccagcgtcg tttgcaggtt
     1201 tttcattgtg cgctgacttt cagaaagcgt actggttgcc tgttcaatca tcggattcag
     1261 tggcagtttg ttgatcttat ccaacgcttc catcagtcgt tgctggattt gcgccaggcc
     1321 gccgctgacg gtcgggataa tctgataacc attaaattca cgaataccgg ttattgcagg
     1381 cgtatttgga tagaaatcga gatcaacata cagtgcgcca gtgaccaggt ttccggtttt
     1441 cagcgatccg cgtaaaccac gtttcaacaa ttcgccaagg tgctcaacaa catccgcatt
     1501 ttcgccaagc tgcattttca gccgctctgg ctcgatacga atcagtaccg gaatacggta
     1561 atcatcgtta aatgtctgac gcatattcgg cgcaaagaat ggcactttgc ttacggtacc
     1621 caggcgaata ccccggaatt ctaccggagc tcccggttgc agaccgcgta ccgaatcttt
     1681 aaagaacatc agataatcaa tgtgatcggt gtacaacgaa tcctgaatgc tcttctgatc
     1741 atcatacaaa acgaaagctg ttttcggtgc cactggctgc cctaaatcca gaccttccgg
     1801 cacatcaaag ctgacaccgc cactaagcag cgttgtcaat gagcccatct ccacacgcat
     1861 tcccgctgac gtcagatcaa ccgcaatgcc actatctttc cagaaacgaa cattgctggt
     1921 caccagtcgg tcataaggtg cattgatgaa cagttgatag ctgatattgc gtttttgcgt
     1981 atcgaaagtg ctggtttcaa ccgaacctac ccgatagcca cggaacagca ccggatcccc
     2041 tggcgagagc tgcccggctt ttttgctatc gagaagcaca cggatacctt tcgcatcagg
     2101 cggggccaac ggtggcgagt ccagcaaatc gtatttatcc attttgctgc ctttcgcgcc
     2161 tggctgcagt tcgatataaa ccccagacag cagcgttccc aggccgctaa tcccttctcg
     2221 accaatctgc ggtttcacca cccaaaagac ggtatcttta tgcagcaatt tttccatacc
     2281 ggaattcagc cgcgctttga tttcaacgtg cgtcaaatca tcagccagtg tggcgctttc
     2341 aaccacgccg acgtcaacgc tacggctttt aatggtggtt ttgccacctt caattccttc
     2401 cgcattcgcg gtgatcaggg tcacttccgg tccctgatgg ctgtaatgat aaaaaagaac
     2461 ccaggcccca atgagcgccg tgacgatagg aaatatccac acgggagacc agttcttcac
     2521 tttctggatt ttggcttccc cattattaga ttccatactg tcaggactcc tcatgctctg
     2581 attcaggttg acgatcccac gacaaacgcg ggtcaaacgt catagcagaa aacattgtca
     2641 ttatgacgac taaagcaaac attaatgcac ccattgccgg ataaatactc attaaacctc
     2701 ccatacgcac cagcgccgag agcaccgcga taacgaaaac gtcaatcatc gaccagcggc
     2761 ctacaaactc aacaacttca taaatcaaat gcattctttc actgtcgcgc ttgccgtgtc
     2821 ctttggcatc ccaacacagc cacgcaatgg cgatcatctt taacgttggc accataatac
     2881 tggccagaaa aatcaccgca gcgacgggat aagagccttc gctccataac agaatgaccc
     2941 cagcgagaat cgtcgacggc atcttcgagc ctaataaatc cgtcaccatg atgggcaaaa
     3001 tattagccgg aagatacagc atgatagacg ttacaagcag cgcgagtgtc cattgcaggc
     3061 tgtttctacg ccgaacgtac cctttggtac tacaacgcgg gcacacgggt tcatcagcag
     3121 gaaggattgc cgtacaacag gaacacgaac gcagcccctg gcgtatcccc gtgacgcctg
     3181 gttttagcgg ctggcgcagt tctggcatcg gggcgatgtc gtcccataac cagcgacgat
     3241 caacgcactg aaaagcgcgc agttgcagga cacaaaataa acaccagggg agaaagctgc
     3301 tgcctacccc aatgctgccg taagccatca gtttaacgaa actgaccagc acacccgcga
     3361 ggaaaatctc cgccattccc caggttttga gttgaaaaag cacccgtgcc agttgctctt
     3421 ttaaacggac cggtaattcc gcgcgattca ccagtaacag aatggttatc agacaaaacg
     3481 cgggaaccag ttgcacaaac aacaggaaaa aggtgccgag gctggcgtag tcctcagaaa
     3541 aaagcacgcc gggaatttcc agtaatgtaa tttcactggt aactcctgca acgttcatat
     3601 tcacaaaagg aaacaagttg gacagcaaca gcatgaacag tgcagccaac gcataggcgg
     3661 tgggacgctg ccgaggggca tcccacgcca cggttaacgt tgtgccacac cggggacatg
     3721 ccgctttctg accatgctca aggcgcggta acgccaccag catgtcacac tgcgagcaca
     3781 ggatgtgctt cgcggcatga tgatgttcgc acattggtac tccttaatga tgcgctattt
     3841 tgcagatgac taacaaagtg ttggttttca tgcctgataa acccagtaca tcaggcaatt
     3901 ttaccgttgt cagcagtctc tgtttaaatc gactattttg cgatcagcca ccatttttta
     3961 acgcttcaag atactcccag cgttcaaagg cttgctccag ctcctgctct gcagcagcca
     4021 tatcagcgag tactttttgc gtctgctcat gcggctgact gaagaaggaa gcatccgcca
     4081 cttgcgtctg taaagcttcc agcttcgcct ccagatcttc aagcaattgc ggtagcagct
     4141 ccagttcacg ctgcaattta tagcttagtt tgctactgct gcgttttaca gtttctgctt
     4201 ttggcgcggc ggcttcttcg ctttttttca ccgcaggctg tttgagcgcc acatactgtt
     4261 cttgctgacc acgggcatca tgataaccgc cgacataacg accaatttta ccgccgcctt
     4321 cgaagatcca gcattctgta acggtgttat cgacaaactg acgatcgtgg ctaaccagca
     4381 ataccgtgcc ctgatagctg tcgatcagtt cttccagcag ttccagcgtt tcgacatcaa
     4441 gatcgttggt cggttcgtca agaatcaata agttgcttgg tttgaggaac aaacgcgcca
     4501 acaacaagcg gttacgctca ccgccagaaa gcgcacgtac cggcgtcatc gcccgttttg
     4561 gatggaacag gaagtcctgc aaatagccca atacgtggcg tggcttgccg ttaaccatca
     4621 cctcttgctt accttcggca aggttatcca tcaccgtttt atcgggatcc agttccgcgc
     4681 ggtgctgatc gaaataagcc acttccagtt tggtgccaac gtgaatacgc ccgctgtctg
     4741 cttgaagctg accgagcatt agtttcagca gcgtggtttt gccgcaccca ttcggaccaa
     4801 tcagggcaat tttgtcgccg cgtagaacct gggcagaaaa atctttcacc agttgcttac
     4861 cgtcaacctg gtagcaaacg tcctccattt cgaaaacgat cttaccggag cggctggctt
     4921 cttccacctg catctttgcg gtacccatca cttcgcgacg ttcaccacgc tcacgacgca
     4981 ttgctttcag ggcgcgtacg cggccttcat tacgggtacg acgtgctttg atcccctggc
     5041 ggatccacac ctcttcctgc gccagtttgc gatcgaactc ggcattttgt aattcttcca
     5101 cgcgcagggc ttcttctttt tccagcaggt actggtcgta attccctgga taggtcacca
     5161 gcttgccgcg atcgagatca acaatgcgcg tcgccatatt gcggataaac gaacggtcgt
     5221 gggaaatgaa aataattgtc ccattgaagg ttttgaggaa cccttccagc cagtcgatgg
     5281 tttcaatatc caggtggtta gtcggttcgt cgagcagcaa tacgcgcgga ttgctcacca
     5341 gcgcgcgtcc taatgccgct ttacgcaacc agccaccgga aagcgacgac agcgcaacat
     5401 taggatctaa tcccagttgc gccagcactt cgttgatgcg gttttccagc tgccacaggt
     5461 tgtggtgatc cagctgttcc tgaaccttcg ccagttcgtt gagatttttc tcgctcgggt
     5521 cgttcatcac caggcgcgaa atatcgtgat agcgtttcag gtattccgct tgttcttcaa
     5581 tgccttcggc aacgaaatca taaacgctac cctcaacgtt acgcggcgga tcctgttgca
     5641 gacgagctac aatcaaatct tgctcgtaaa taatgcgacc gtcatccagc ccctgttcac
     5701 ggttgaggat cttcattaac gtcgatttgc ctgcgccgtt acgacccacc agacaaacac
     5761 gttcgttatc ttcgatatgc agttctgcgt tatcgagaag cggcgcgtcg ctgaacgaca
     5821 gccatgcgcc atgcatactg attaatgaca ttactattcc tttcaggctg cggtaatcag
     5881 ccagcagttg tggatctggc ggttacgggc gaaatcctgg gagagcgttt tttgcgtaat
     5941 ttcttgtgct ttcagtccca gtttcgccag gccgtcgaga tccatacgga agccgcgttt
     6001 gttgttcgag aacatgatcg tcccacctgc acgcagcaga cgtttcaaat ctttcatcag
     6061 cgccagatga tcgcgctgaa catcaaacgc atcttccatt cgttttgagt tagagaaggt
     6121 tggcggatcg ataaagatca gatcgaactg ttcatttgcc tcacgcagcc acgccaggca
     6181 atcggcctga atcaggcgat gcgcacgccc ggttaggcca ttcagacgca ggttgcgttc
     6241 tgcccactcc agataagtac gcgacatatc cacggtggtg gtgctgcgcg cgccgcctaa
     6301 tcccgcatgc acggtggcgc taccggtata ggagaacagg ttgaggaaat ctttgccttt
     6361 gctcatctga ccgagcatac gacgggcgat acggtgatcg aggaacagac cggtatcgag
     6421 gtaatccgtc aagttcaccc acaagtgagc gttatattcg gtaacttcaa gaaactcgcc
     6481 cttctcgccc agtttctggt actgattttt gcccttctgg cgttcacggg ttttcagcac
     6541 cagtttgttt ggcgcaatcc ccagtaccga aatggttgca gcgataatat cgaacagacg
     6601 ctgacgcgct ttgtgcgcat caatagtttt tggcggcgca tactcctgca ccaccaccca
     6661 gtcggcataa cggtcaacgg caacgttata ttccggcaga tcggcatcat acaggcgata
     6721 acattcaatc ccttcctgac gtgcccactt ctcgaatttt ttgaggttct tacgcaggcg
     6781 gttggcgtag tcttccgcta ccattgccgg tttgctgtct ggtgtgcttt cggcaacgtg
     6841 gtagtttttc tgtacgcagt ccagcgggcc gtttttcgcc ttgtactgtt tgtctgcacg
     6901 cagctgcagg cagcttagca gatccggcga ggcactaaac aaagagagat tccagccacc
     6961 gaactggttt ttcatgattc ggcccagcag actatgcagc gcaatcagcg ccggttcgct
     7021 gtccagacgt tcaccgtatg gcgggttgct caacactgta ccgtacggcc ctttcggcag
     7081 cggattggtc agttgcgcga catctttcac ctcaaaggtg atcagttcac caatccccgc
     7141 aagacgggcg ttagtgcgcg cacgttgaat cacccgggcg tcgctgtccg aaccgtaaaa
     7201 atgagagcta tactctgcca ggcctttacg ggcgcgagtt tgcgcttccg ctttcacttc
     7261 ctgccagata gcttcatcat gctgcgccca gccgctaaag ccccaacggc cacggtgcaa
     7321 gcccggtgcg cggtcggtcg ccagcatcgc tgcttcaatc agcaacgtac cagaaccaca
     7381 cattggatcg agcagtggtg ttcctggctg ccagccggat cgcatcacaa tcgcggctgc
     7441 cagggtttct ttgatcggtg caataccagc acgatcacga tagccacgca gatgcaagcc
     7501 atcaccactg agatcaaggg cgatattggc ggtttcttta tgcagccaga cgttaacgcg
     7561 aatatccggc gcatcgcgat caacattggg acgtggcaga tttttccgcg tgaaagcatc
     7621 gacgatcgcg tctttcactt tcatcgcgcc gtactggctg ttgcgaatgg tgtcatttaa
     7681 accactgaaa tggacagcga aggtcgcgcc aggattaaac atctctgtcc agttgatcgc
     7741 ctgaacgccg agatagaggt ctaaatcact gtaaacctta cactcgccca gcggcaacat
     7801 aatacgcgag gccaggcggc tccacatcag gctctggtaa acaagccgtg tgtcgccctt
     7861 gaaatggacc ccaccctgaa ccacctggca ttcaacggcc cccaggtttt ccagttcagt
     7921 ttttaacagc tcttccagcc cacgggccgt actggcaaac agagaattca tatcgtcact
     7981 tttactctaa gaaaattgtt gcgcattata gctaataagc gcgtcatgtc ataaagttga
     8041 gggcttattt tcatttgagg accgcaccgt ggcgacatta acccggcttt ttattcatcc
     8101 tgttaaatcg atgcgcggca ttggtcttac acataccctg gcagatgtca gtggtctggc
     8161 cttcgatcgc atctttatga tcacggaacc tgacggtacg tttattaccg ctcgccagtt
     8221 tccgctaatg gtacggttta ctccttcacc cgtgcatgat ggcttgcatc tcaccgcacc
     8281 agatggcagt agcgcatatg ttcgttttgc tgatttcgcc acacaagacg caccaaccga
     8341 agtttggggc acacatttta ccgcgcgaat tgcgccagat gcgatcaaca aatggctaag
     8401 tggatttttc tcccgcgaag tgcaattacg ctgggtgggg ccacaaatga cccggcgcgt
     8461 gaaacgccac aacactgtac ccctgtcatt tgctgatggc tatccttacc ttcttgctaa
     8521 cgaggcctcg ttacgtgctc tccaacaacg ttgtccggcc agtgtaaaaa tggagcaatt
     8581 ccgccccaat ctggtggttt ccggtgcatc ggcctgggaa gaagatagct ggaaagtgat
     8641 tcgcattggt gatgtcgtgt ttgatgtggt taagccttgt agccgctgta ttttcaccac
     8701 cgtcagccca gaaaaagggc aaaaacatcc ggcagacgaa ccattaaaaa cgctgcaatc
     8761 tttccgtact gcccaggata acggcgatgt cgattttggt cagaatttaa ttgcccgtaa
     8821 tagcggcgtg attcgcgttg gcgatgaggt ggaaattctg gcaacggctc cagctaaaat
     8881 ttacggcgca ggtgccgctg atgatactgc caacatcacg caacaaccgg acgcaaacgt
     8941 agatattgac tggcagggac aggcatttcg tggaaataac caacaggtgc tgctggagca
     9001 attagaaaat cagggaattc gtatccctta ttcttgccgc gcaggcattt gtggaagttg
     9061 ccgtgttcag cttttagaag gcgaagtcac gccgctaaaa aaatcagcaa caggcgatga
     9121 tggcactatt ctttgctgta gttgtgtacc gaagactgcg cttaagctgg cgcgttagac
     9181 tgcctgttcg aggctgaagc tgtcaacatt aacctgcggt ttcagcctgt cgttcatgat
     9241 tttaatggcg tcgcccaact gcatcgcgcg acccgcaata acaacgcagg gttgtgccag
     9301 tagacatagc gcggcattct cgccagattc aacgaccagt aaattcactt gctcgtgagt
     9361 gtcgctcaac caaacgtagg ccgcatcgcc cggcatgggc atccacattt caccatgaga
     9421 tacaaaatgc cagcttttcg gcatttgcgg tttgagataa cgaattgcta ccagtgcatt
     9481 cagcaccagt tcggcttttt gttctttaga taaattaaaa tcgcggcact tttcttcaaa
     9541 agagaaatag agcgcggcgt catcaacgca aaaacctgcg ggagaaaagg catctggagt
     9601 caacattttg cgcgcaaaac gtgagcgaaa tagcatacca ttggctaaat cgagcatcat
     9661 tcgatcgtgc tcttcatcgt aataccaacg ccagttatcg tctggtttaa ttcgcatctg
     9721 ccactcccca accttataaa accgcagcga cacaatccat tgtcgcttcg cttaataacc
     9781 tcaacaataa aagtacaaaa tgtctaaata agcaacagcg atgaatataa aacaaccagg
     9841 gctgaaaata aagccctgat tatcttataa agaggaattt gaagaaataa ttagatatgg
     9901 gtaacgattt ctttaatcag cggcggacct ttaaaaataa aaccagaata aatttgcacc
     9961 agtgaggcac ccgcagcaat cttttcacgc gcagcgataa ccgagtcaat gccgccaaca
    10021 ccaataatcg gcaagcgtcc gtttaattcc tgtgacaagc ggcgaataat ttcggtgctt
    10081 tttaactgaa gcggacgacc acttaagcca ccggtttgat cacaattttt cattccctga
    10141 accagagaac gatcgagtgt ggtattggtt gcaataacgc catcaatatt atggcgaact
    10201 aaactatcgg caacctggat caattcttct tcagaaagat ccggcgcgat cttcactgcg
    10261 atcggcacat atttatggtg catcacttgc aaatcatttt gtttattttt aatcgcagtt
    10321 aagagatcat ccagcgcttc accatattgc agtgtgcgta atcctggggt atttggcgat
    10381 gaaatattga tggcgatata tcccgcatag gcatagattt tttccataca aatcagatag
    10441 tcatctttac cctgctccac cggcgtatct ttatttttgc cgatgttaat acccagaacg
    10501 ccgtcataat gggctttttt tacgttctct acgaggttat caacgccaag attattaaag
    10561 cccatacggt tgatcaaacc ttcggcatct accagacgaa agagacgcgg cttgtcatta
    10621 cctggctggg gacgtggcgt gacggtaccg atctcgatgg atccaaatcc catcgcgcct
    10681 aacgcgtcaa tgcactcccc gtctttatca agaccggctg ccagaccaag cggattttta
    10741 aacgtcaggc ccatgcagtt aacaggtttc gcaggcactt tctgccgcac cagtgcttca
    10801 aacggcgttc ctgtaatacg gcgtaattgc tgaaaagtaa actcatgagc gcgctctgga
    10861 tcgagctgga aaagggcttt acgaacgaag gggtagtaca tgaactctcc tggattcccg
    10921 gtgtgcaaac cgggggcgta ttatgtgcga gttcggacca aaagggaatt gacctgcggc
    10981 aataaatagc aaacgttttc ttattgcgct ttttttatgc tttttttggc ttttcttcct
    11041 cagataaatc atttcgactc gcggacgccc tctgtcagac tgaccgaatt gttatcaatg
    11101 ttaacaaaac aagaacaatt ggttataagg agagagtatg cgtgtcatca ccctggcggg
    11161 tagtcctcga tttccttctc gctccagctc cttgctggaa tatgcgcggg aaaaactaaa
    11221 tggcctggat gtagaggttt atcactggaa tttgcaaaac ttcgtcccgg aagatctgct
    11281 ttatgctcgt ttcgatagtc cggcactcaa gaccttcacc gaacagctgc aacaggccga
    11341 tgggctgatt gtcgccacgc ctgtgtataa agccgcctat tccggtgcac tgaaaaccct
    11401 gctcgacctg ctgccagaac gtgcgttgca aggcaaagtg gtgctgccgc tggcgacagg
    11461 cggcaccgtg gcccatctgc tggcggtcga ttatgccctt aagccagttt taagtacgct
    11521 gaaagcccag gagatcctgc acggcgtgtt tgccgatgac tcgcaagtaa ttgattacca
    11581 acacaaaccc cagttcacgc caaatctgca aactcgtctt gataccgcgc tagaaacttt
    11641 ctggcaggcg ttgcaccgcc gcgatgttca ggttcctgac ctactgtctt tgcgaggtaa
    11701 tgcccatgcg taagatcatt aaactggtgc tggcgggttt acttagcgtc tctacgattg
    11761 cggttgctgc agaatcctcg cctgaagcgt tacgtatagg ctatcaaaaa ggcagtattg
    11821 gtatggtgct ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa acaaaaatct
    11881 cctgggtcga gttccccgct ggcccacaga tgctggaggc gttaaacgtt ggcagtatcg
    11941 atctcggcag taccggggat attccgccaa tctttgccca ggctgccggg gctgatttgg
    12001 tatacgtggg cgtcgagcca ccgaagccca aagccgaagt gattctggtg gcagaaaaca
    12061 gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt tgcctttcag aaaggttcca
    12121 gttcgcacaa tcttttactg cgagcccttc gccaggccgg gcttaaattc acggatattc
    12181 agcccactta cctgacgcct gccgatgccc gcgccgcgtt ccagcaaggt aacgttgacg
    12241 cctgggctat ctgggatccc tactactccg ctgcattatt acagggcggc gtgcgggtgc
    12301 tgaaagacgg caccgatctc aatcaaactg gatcgtttta tctggcagct cgtccgtatg
    12361 cagaaaaaaa cggcgctttt attcagggtg tgctgacaac ctttagtgag gccgatgcgt
    12421 taacccgcag ccagcgcgaa caaagcatcg ctttactggc aaaaacgatg ggcttaccgg
    12481 caccggttat tgcctcgtat ctggatcatc gtcctcccac caccatcaaa ccgttgagcg
    12541 ctgaagttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaac cgtctggtgc
    12601 cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac tcaactggaa ggaaaacaat
    12661 tatgagtctg aatatgttct ggtttttacc gacccacggt gacgggcatt atctgggaac
    12721 ggaagaaggt tcacgtccgg ttgatcacgg ttatctgcaa caaattgcgc aagcggcgga
    12781 tcgtctgggc tataccggtg tgctgatccc gacggggcga tcgtgtgaag atgcgtggct
    12841 ggtggcggcg tcgatgatcc cggtgacaca acggctgaag tttcttgtcg ccctgcgccc
    12901 cagcgtaacc tcacctaccg ttgccgcccg ccaggccgcc acgcttgacc gtctctctaa
    12961 cggacgtgcg ttgtttaacc tggtcacagg cagcgatccc caagaactgg caggcgacgg
    13021 cgtgttcctt gatcatagcg agcgttacga agcctcggca gaatttaccc aggtctggcg
    13081 gcggttgttg cttggcgaga ctgtggattt caacggcaaa catattcatg tacgcggggc
    13141 caaactgctc ttcccgccga ttcaacagcc ttatcctccg ctttactttg gcggatcgtc
    13201 agatgtcgcc caggagctgg cggcagaaca agttgatctc taccttacct ggggcgaacc
    13261 accgaaactg gtaaaagaga aaatcgaaca cgtgcgggcg aaagctgccg cgcatggacg
    13321 caaaattcgt ttcggtattc gtctgcatgt gattgttcgt gaaaccaacg acgaagcgtg
    13381 gcaggccgcc gagcggttaa tctcgcatct tgatgatgaa actatcgcca aagcacaggc
    13441 ctcattcgcc cgaacggatt ccgtaggaca acagcgaatg gcggcgctac ataacggcaa
    13501 gcgcgacaat ctggagatca gccccaattt atgggcgggt gttggcttag tgcgcggcgg
    13561 tgccgggaca gcgctggtgg gcgatggtcc tacggtcgcc gcgcgaatca acgaatacgc
    13621 tgcacttggc atcgacagtt ttgtgctttc gggctacccg catctggaag aagcgtatcg
    13681 ggtcggcgag ttactgttcc cacatctgga tgtcgccatc ccggaaattc cccagccaca
    13741 accgctgaat ccacaaggcg aagcggtggc gaatgatttt atcccccgta acgtcgcgca
    13801 aagctaagga acacgatgat ggcaacgcca gtgaagaagt ggttattgcg cgttgccccc
    13861 tggtttttac cggtgggcat cgtggcggtg tggcaactgg cctcctcggt tggctggcta
    13921 tcgacgcgta ttttgccctc accggaagga gtggtgatgg cgttctggac gctctctgcc
    13981 agcggcgaac tgtggcagca tctggcgatc agctcctggc gtgcactgat tggtttttca
    14041 attggcggat cgctgggatt aattctgggg ctgattagcg ggctgtcacg ctggggtgag
    14101 cgactgctgg atacctcaat tcagatgttg cgcaacgtgc cgcatctggc gctgatcccg
    14161 ctggtgattt tgtggtttgg cattgatgaa tccgcaaaaa tctttctggt ggcgctcggt
    14221 acgctattcc ccatttatat caacacctgg catggcatcc gtaatatcga ccgtgagctg
    14281 gtggagatgg cacgtagcta tggattatcc ggcatgccgc tgtttatcca tgtgatcctg
    14341 cctggtgccc tgccctcaat tatggtcggt gtgcgttttg ctttgggcct gatgtggctg
    14401 acgctgatcg ttgccgaaac tatttctgcc aattccggca ttggttatct ggcgatgaat
    14461 gcgcgggagt ttctgcaaac ggacgtggtg gtagtcgcca ttattcttta cgccctgctc
    14521 ggcaaacttg ctgacgtcag cgcgcagctg ttagagcgtc tctggttgcg ctggaacccg
    14581 gcttatcatt tgaaggaggc cacggtatga atactgctcg tctgaaccag ggcacaccat
    14641 tgttgctcaa tgcagtaagc aaacattacg cggaaaatat cgtcctgaac caactggatt
    14701 tacatattcc agcaggtcag tttgtggcgg tggtgggccg cagcggtggt ggcaaaagta
    14761 ccctgctgcg cctgctggca ggtctggaaa cgccaaccgc aggcgacgtg ttagcgggca
    14821 ccacaccgct ggctgagatt caggatgata cgcgaatgat gtttcaggac gcgcgtctgc
    14881 tgccgtggaa atcggtgatt gataacgttg ggttaggcct taaaggccag tggcgcgatg
    14941 ccgcgcgtcg ggctctggct gcggtaggac tggagaatcg cgcgggggaa tggcctgccg
    15001 cactttctgg cgggcagaaa caacgtgtgg cgctggcaag ggcgctgatt catcgaccag
    15061 gtttattgtt gcttgatgaa ccactcggtg cgctggacgc tttaacgcga ctcgagatgc
    15121 aggatttgat tgtatcactc tggcaggagc acggctttac cgtcttgctg gtgacgcatg
    15181 atgtgagcga agcggtggcg atggctgacc gggtgttgtt aattgaagag ggaaaaattg
    15241 gtctggattt gacggtggat atcccccgtc cgcgccggtt agggtcggta cgattagcag
    15301 agctggaagc ggaagtattg cagcgggtga tgcagcgtgg tgaatttgaa
//
LOCUS       DAOFSV010000106        15212 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.106, whole genome shotgun sequence.
ACCESSION   DAOFSV010000106 DAOFSV010000000
VERSION     DAOFSV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.106"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(178..253)
                     /locus_tag="QBH27_003382"
     tRNA            complement(178..253)
                     /locus_tag="QBH27_003382"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(218..220),aa:Phe,seq:gaa)
     gene            complement(359..1066)
                     /locus_tag="QBH27_003383"
     CDS             complement(359..1066)
                     /locus_tag="QBH27_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDQ7015660.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1459..3594
                     /gene="speC"
                     /locus_tag="QBH27_003384"
     CDS             1459..3594
                     /gene="speC"
                     /locus_tag="QBH27_003384"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDQ7015661.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFCVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            complement(3643..4899)
                     /gene="nupG"
                     /locus_tag="QBH27_003385"
     CDS             complement(3643..4899)
                     /gene="nupG"
                     /locus_tag="QBH27_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDQ7015662.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5101..6180)
                     /gene="mltC"
                     /locus_tag="QBH27_003386"
     CDS             complement(5101..6180)
                     /gene="mltC"
                     /locus_tag="QBH27_003386"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDQ7015663.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6245..6520)
                     /locus_tag="QBH27_003387"
     CDS             complement(6245..6520)
                     /locus_tag="QBH27_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDQ7015664.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6548..7600)
                     /gene="mutY"
                     /locus_tag="QBH27_003388"
     CDS             complement(6548..7600)
                     /gene="mutY"
                     /locus_tag="QBH27_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDQ7015665.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIATANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7761..8480
                     /gene="trmB"
                     /locus_tag="QBH27_003389"
     CDS             7761..8480
                     /gene="trmB"
                     /locus_tag="QBH27_003389"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDQ7015666.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8480..8806
                     /locus_tag="QBH27_003390"
     CDS             8480..8806
                     /locus_tag="QBH27_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDQ7015667.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            8829..8969
                     /locus_tag="QBH27_003391"
     CDS             8829..8969
                     /locus_tag="QBH27_003391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015668.1"
                     /translation="MQNCLMRYAYQAWKDAQSSRPDKAFTPHPAWMTCTLLSIWGQQM
                     LA"
     gene            8990..9709
                     /locus_tag="QBH27_003392"
     CDS             8990..9709
                     /locus_tag="QBH27_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDQ7015669.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9885..10931
                     /gene="ansB"
                     /locus_tag="QBH27_003393"
     CDS             9885..10931
                     /gene="ansB"
                     /locus_tag="QBH27_003393"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDQ7015670.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11048..12055
                     /locus_tag="QBH27_003394"
     CDS             11048..12055
                     /locus_tag="QBH27_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDQ7015671.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVSIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12111..13412)
                     /locus_tag="QBH27_003395"
     CDS             complement(12111..13412)
                     /locus_tag="QBH27_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDQ7015672.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLITLMMITYIPQISLFIPQLLGLM"
     gene            complement(13412..13915)
                     /locus_tag="QBH27_003396"
     CDS             complement(13412..13915)
                     /locus_tag="QBH27_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDQ7015673.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(13960..14946)
                     /locus_tag="QBH27_003397"
     CDS             complement(13960..14946)
                     /locus_tag="QBH27_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDQ7015674.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGEEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEADQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4029 a         3617 c         3855 g         3711 t
ORIGIN      
        1 ttaaagaggg tacaaattct gtcgaactgg ggtatacccg caattatacc cacacaatgg
       61 ggttgatgtg gggggaatct ggttgagttc ggtagaattg atttggaggc agaacgctta
      121 aattgtggcg ttctgaaacg aaaaacggac ctccgtggag gtccgttgat atgaacttgg
      181 tgcccggact cggaatcgaa ccaaggacac ggggattttc aatcccctgc tctaccgact
      241 gagctatccg ggcaacgggg cgcattaaac ctgattcgcc tcgtctcgtc aatcaaattt
      301 cttcaattta ctgcagactg cacaatctat catcactttg ccgctaatgc acgcattgtc
      361 aggcaaacca ggcggtccag gcggcggaaa ggggcattgc cagaaggagt gctggaagca
      421 tgttgaccac cgggaacatt ttaatgccac agatgcgtaa tccggttgcc agcagcaata
      481 aaccgcctac ggcgctgaag tctgccatca tcgacggtgt ggtcagcggt aatatcagcg
      541 cggcagccca cgccagcgtt aactggatga tcagtaatgg gatactaatc accgataccg
      601 caatacccag cgagcaggcg aagatcatcg ccgtaaagaa atcaagaaat gacttggcga
      661 ttaaaatact cggatcgccg gtcatccctt cgttcatcgc cccgaagatc ccggtgccgc
      721 tggcgcaaaa caggacaata atcgcgacat aattctgaat aaaagattca tgcgctggct
      781 tcttacgtga gtggcgaaac agattttgtg ctttggtgac cgcggtattg acgccttttt
      841 ccagcagaca aatttcgccg attaatgcac cgagtaaggt tgctaaaacc atcgccggaa
      901 ggttggcgca tttcaccacc agtaaaatac caatccccag cgatgccaga ccaaaaattg
      961 atgtcatgga gacgcggata cgttccggta agcgttggct gagcagtgcg ccgagaacgc
     1021 cacccagtaa gactgcacta gcgttgataa aagggccgat gaccacaaga gttcctgtta
     1081 actgtattgt ttgattttct tattatgaac tttttgcctt gttgatggct tgtctggtcg
     1141 caaagtcctg gcttgcacgc tttagcgaaa ggtgccatga ttgcgcgaat tttctcctct
     1201 ctgtacggag tttgcccgat gcacgccacc tccttacatt ctctcgctta tcgccgtttc
     1261 gcgcgaaact cctccctttt tctgctcatg cggtgaagtt aacgcacgct cactgcagga
     1321 caacagtaaa atcagagcgt ttctgctttt actgatgtct ggcggtcgga gctggtgacc
     1381 agtttgaccc atatctcgtg gggcagggtt tttcaccttg tccggtattc ttacttcccc
     1441 gaaacgggtt ttgcgcttat gaaatcaatg aatattgccg ccagtagtga actggtatcc
     1501 cgactttcta ctcatcgccg cgtggtggcg ttggcagata ctgattttac ggacgtcgcg
     1561 gcagtcgtca ttaccgctgc ggatagtcgc agtggcattc ttgcgttgct taagcgcacc
     1621 ggttttcatc taccggtgtt tttgtattcc gaacatgttg ttgaattacc tgcgggcgtt
     1681 acggcggtaa tcaacggcaa cgagcagcag tggctggagc tggaatccgc agcctgtcag
     1741 tatgaagaga atttgctgcc accgttttat gacacgctga cgcagtacgt tgagatgggt
     1801 aatagcacct ttgcttgccc tggacatcaa catggtgcgt tctttaaaaa gcatcctgcc
     1861 ggacgccatt tttacgattt ctttggcgag aacatctttc gcgccgatat gtgtaacgct
     1921 gacgtaaaat tgggcgatct gctgattcat gaaggatcgg cgaaagatgc gcagaaattc
     1981 gcagccaaag tctttcatgc cgataaaacc tattttgtgc tgaacggcac atcggcagcg
     2041 aataaagtgg tgacgaatgc gctgttaacg cgtggcgatt tggtgctctt cgaccgtaac
     2101 aaccataagt cgaatcatca cggcgcgttg attcaggcgg gggcgacgcc ggtctatctg
     2161 gaagcttcac gtaacccgtt tggtttcatt ggcggtattg atgcgcactg ttttaatgaa
     2221 gagtatctgc gccagcaaat tcgcgacgtt gcgccagaaa aagccgacct gccgcgcccg
     2281 tttcgcctgg cgattattca gctgggaacc tatgacggca ctgtctataa cgcccgtcag
     2341 gtgatcgata ccgttgggca tctgtgtgat tacattctgt ttgattccgc gtgggtcggt
     2401 tacgaacagt ttatcccgat gatggcggat agctcgccgc tgctgttaga acttaacgaa
     2461 aacgatccgg ggatctttgt gacccagtcg gtgcacaaac agcaggcggg attctcacag
     2521 acgtcgcaga tccataaaaa agataaccat atccgtggac aggcgcgttt ttgcccgcat
     2581 aagcggttga ataatgcctt tatgctccat gcttctacca gcccgttcta tccgctgttc
     2641 gccgcgctgg acgttaacgc caaaattcat gaaggggaga gtgggcgtcg gctgtgggcg
     2701 gagtgcgttg cgttggggat tgaagcccgg aaggcgattc ttgcgcgctg taagctgttc
     2761 cgcccgttta tcccgcccgt tgttgatggc aaattgtggc aggattatcc gacgtcagtg
     2821 ttagccagcg accgccgttt tttcagtttt gagccggggg cgaagtggca cggctttgaa
     2881 ggatatgccg cggatcagta ttttgttgat ccgtgcaagc tgttactcac cacgccgggt
     2941 atcgatgccg aaaccggcga atatagcgat ttttgcgttc cagcgacgat tctggcgcac
     3001 tacctgcgtg agaacggcat tgtgccggag aagtgcgatc tcaactccat tctgttctta
     3061 ttaactccgg cggaaagcca cgagaagctg gcacaactgg tggcgatgct ggcgcagttt
     3121 gaacagcata ttgaggatga ctcgccgctg gctgaggtgt tgccgagcgt ttataacaaa
     3181 tatccggtgc gctatcgcga ctacaccctg cgtcagttgt gtcaggagat gcacgatctg
     3241 tatgtcagtt tcgacgtcaa agacctacaa aaagcgatgt tccgccagca gagtttcccg
     3301 tcagtggtga tgaatcccca ggatgcgcat agcgcttata ttcgcggtga agtggagttg
     3361 gtgcggattc gtgatgccga agggcgaatt gcggcagaag gggcgttgcc ttatccacct
     3421 ggcgtgcttt gcgtggtacc cggggaagtc tggggtgggg cggttcaacg ttatttcctt
     3481 gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc cggagttgca aggtgtctat
     3541 agcgaaaccg atgcgaatgg catgaaacgg ttgtacggtt atgtgttgaa gtaagaataa
     3601 aaaaacgggt caccttctgg cgacccgttt ttctttgcgt aattagtggc taaccgtctg
     3661 tgtacctgtc gggacacgaa cgtgtttata tttgaacatc gccatgaacg cgaaggccag
     3721 aaccacggag taacctgcga aaatcagcca tacggtctgc cagtcggtaa tgccgttttg
     3781 ggtgtacatc tcaaccactt taccgctcac gatgccgccg aggatacagc cgaagccgtt
     3841 ggtcatcatc aggaacatcc cctgcgcgct ggcgcgaatt gccggactaa cttctttttc
     3901 gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga
     3961 cagtaccagc agtacagtac cgaacggagt cgggtcgccg taagcaaaca gagcaaaacg
     4021 caggatccac gctacaatac tgatcatcat tacgttctta ataccgtagc ggcttaagaa
     4081 gaacgggatg gtcagaatga acagggtttc agagatctgc gaaatcgaca tgatgattga
     4141 cgcatgctgc acgatgaagc tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa
     4201 ggtattaccg aacatgttgg taatctgcag ttccgcgccc agcagcattg agaagatgaa
     4261 gaagattgcc atacgcttgt ttttaaacag cgcgaatgca tcgaggccca gcagggttgt
     4321 ccagctctga ttcgcttgct gtttagcaac cggaatatgc ggcagagtca gggtaaacag
     4381 aaccagaacg gcggaaagag ctgcgccaat atacagctgc atgtggctta attcgaagcc
     4441 ggacaggctc accacccaca ttgccatgat aaagccgatg gtgccccaga tacggattgg
     4501 cgggaagtca gtaacgatat ccatcccggc attttgcagg cgatagtaag agatggtgtt
     4561 gattaaccca agcgttggca tataagcaaa cgagttaatc aatatcacaa ggaacatcgc
     4621 ctccggcgta gtgacctctg ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat
     4681 ggcatagacc catttcgcac ttaaccattt gtcggccaca atccccagca gcgcaggcat
     4741 aaagaccgcg gcgataccca gtgagctata aactgcgccg atagaagcac cgtcaaactt
     4801 cagggtaaca aacatatagg agccgagggt cgtcagccaa cttccccaca gacagaactg
     4861 cagaaaagag aggattttca gctgcagctt aagattcatg ttaatttcct cacatcgtga
     4921 tgcggatgag tgtttgaaaa agcacatttg accgtgcgag atgatgcgat tctatcaact
     4981 tcgcggactg gttttttgtt acctgtggca aataattgca aacattccca atttgcaaaa
     5041 cgaaattgtg atgtggataa catttttacc cctgagcatc gtcaggggcg gttagtggaa
     5101 ttatcggcgg cggtaagatt tttgcgcggt attcacttta taaagataac ggcgagattc
     5161 cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg cccggcgtca tggtgttaat
     5221 aatattggca gcctgaatct tatcattcga aaagactcgt agcacgctgc ctgcgccgcc
     5281 gttataggcg gtgatgacgg cataacgccg cgatgttggg ttatcaattc cgccgagata
     5341 aacgttgttc agcatcgcca gatacgcggt gccggtatca atattgctgg caggatcaaa
     5401 caagaaactg cggctcggcg tgccggattt cccctgcgag cggaacacat ctttcccggc
     5461 ggtatgttgt accacctgca ttaatcccag cgcatcggaa cggctgaccg catacgggtt
     5521 aaaggaagat tcagtctgca taattgccag aatcagcgac tcatcaacgc catatttgcg
     5581 tgacgcctgg cggaccatac cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac
     5641 catgttaatg gtgacgctgt agatgatacg cagcccgttg ctgcggctct tcagacggtt
     5701 tttcagcaga taatccgcga agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt
     5761 gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc
     5821 ggaatagaga tcgaccgaac tcggatcgtc ccccatcagc aacgttttga ttattgcccg
     5881 gcgcaaatgc gcggcaggtt ctgtcccggc gatggtttcg atagtaatcg taccgtcatc
     5941 gaagttgatg tggctgcggg tctgatattg atcggtgtat ttcacgtagt ccttaggacc
     6001 ggcgatcacc acctctttga agccccagat gttctcaata ttgtgggcaa attgccccat
     6061 cagaatatca aaaccgttgg tatctttgac ccaggcttcg ttataggtat cgcctttttt
     6121 ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc agcgcgagat attttttcat
     6181 cattccgggt gcgtgttgtg tttgttttta tgcaagttgc cggaggcgtg ctccggcact
     6241 gtttttattt tttatcttcc ggcgtatagc cctcgatatg cacctcttta ccctcgaaca
     6301 ggaagttgac catctcctgc tccagcagct tgcggtgctc ggcattcatc atgttgagtt
     6361 tcttttcatt aatcagcatg gtttgtttgt gctgccactg cgcccaggct tctttggaga
     6421 tctcgttata gatgcgtttt cccagctcgc cggggtacag ctgaaaatcc tgaccttctg
     6481 cttcacgttg caggaaagta caaaaaatcg ttctgctcat aaatcatcct ctttatcgac
     6541 tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa caaacgctcc acgggagccg
     6601 ccaggccaac tgacggcggt tgcgctaagt tataccagag cgcattgtct tcatccatgc
     6661 agccggtgaa tgacgacacg ggaagccaca taggcacaat atctaagtgg aaatggctga
     6721 aggtatgccg aaacgcggtc agttgcgtca ggttatcggc agcaatctgc cgttgcgcca
     6781 gccactgccg caaactttct tcgtcggcaa actgtgggaa acagtataaa ccgccccaca
     6841 atccactcgg cgggcgctgc gccagcaata cttcatcttc gtgctgtaac agcagaaagt
     6901 agccggtgcg ctccggcagc gtctgtttcg gttttttgcc cggataaagc gaccagctat
     6961 tgttggcggt ggcaatacat ccgttttgta gcggacagag cgaacatttc ggcttcgagc
     7021 gcgtacaaat catcgcgcct aaatccatca tcgcctgatt aaaccgttcc acgccgacag
     7081 cgggcgtcac ctgctcgctc aaactccata atttattctc gacctctttt ttcccgggcc
     7141 agccgcttac agcatagcag cgcgccagca cccgtttgac gttaccgtcg agaatcggaa
     7201 agtgcttacc cagagaaagc gagagaatcg cgcctgcggt ggaacgcccg acgcccggta
     7261 acgccgcgac ttcttcaaag gtttccggga atttaccgcc gtgtaaggtc gccacttgct
     7321 gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga
     7381 gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt tgggaagcgc gccataaagc
     7441 gttcaaaata ggggataacg gtcgcaacct gagtttgttg caacatcact tctgagagcc
     7501 atactttgta gggcgtcttg tcaatttgcc agggcagcgt tttccgcccg tatttatcgt
     7561 accagtccag aacctgggct gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg
     7621 ttgggggcaa gattgcagca cagcgacggc agggtgtaaa ccggaacttt ccgcagccac
     7681 ggccttatca tgcttgcatc cggcaactaa ctttggataa tgcccgtttt cagaacactt
     7741 tcacaagcga ctaaatcttt atgaaaaacg acgtcatctc accggaattt gatgaaaacg
     7801 gccgcccact gcgccgtatc cgtagttttg tgcgccgcca ggggcgactg accaaaggcc
     7861 aggaacatgc gctggaaaac tactggccgg tgatgggcgt tgagttcagc gaagatatgc
     7921 tggatttccc tgcgcttttt ggccgtgaag cgccggtgac gcttgagatt ggtttcggca
     7981 tgggggcgtc gctggtggca atggctaaag atcgccccga gcaggacttc ctcggcattg
     8041 aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca
     8101 acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca taaaatgatt cctgacaatt
     8161 cattgcgcat ggtgcagctc tttttccctg acccgtggca caaagcgcgc cataataaac
     8221 gccgtatcgt tcaggtgccg tttgccgaac tggtaaaaag caaactgcag ctggggggcg
     8281 tattccatat ggcgaccgac tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt
     8341 ctattgacgg ttataaaaac ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac
     8401 gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg tcacggagta tgggacttaa
     8461 tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca
     8521 catcgacgaa ttccaggaat taggattttc ggtggcatgg cgattcccgg aaggtacatc
     8581 ggaagaacag attgataaaa ccgttgatga ctttattaac gaggttatcg aaccgaacaa
     8641 actggccttt gacggcagcg gttatctggc ctgggaaggt ctgatctgca tgcaggaaat
     8701 cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag tggctggaag agcgcaaact
     8761 gggcgaggta cgcaccagcg aacttttcga cgtttggtgg gactaagaaa acatacgggc
     8821 gatgacaaat gcaaaactgt ctgatgcgct acgcttatca ggcctggaaa gatgcacaat
     8881 cgagtaggcc ggataaggcg tttacgccgc atccggcatg gatgacatgt actttgttat
     8941 caatctgggg ccagcaaatg ctggcctgat ttgttcttga gggaagacta tgatgcgcaa
     9001 aatgctgctg gcggcagcac tttcagtgac ggcaatgacc gctcacgccg actaccagtg
     9061 cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa accgtgcagg tgaagggcga
     9121 aaacggcaat ctggtgatca cgccagacgg caacgtgatg tataacggta agcaatattc
     9181 cctgaacgcc gcccagcgcg agcaggcgaa ggattatcag gctgaactgc gtagcaccct
     9241 gccgtggatt gatgaaggcg cgaaaagccg cgtcgagaaa gcccgtattg cgctggataa
     9301 aattatcgtt caggagatgg gcgaaagcag caaaatgcgc agccgtctga ccaaacttga
     9361 tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt
     9421 tcactataaa gccattgatc aggttcgcgc cgaaggccag caattagtga atcaggcaat
     9481 gggcggaatt ttacaggaca gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg
     9541 tggtaaccca ttacagaatg tgctgggaag cctgggcggt ctgcaatcct caatccaaac
     9601 cgaatggaaa aagcaggaaa aagatttcca gcagtttggc aaagatgttt gtagccgcgt
     9661 tgtgactctg gaagatagtc gcaaagccct ggtcgggaat ttaaaataat cctctatttt
     9721 aagacggcat aatacttttt tatgccgttt aattcttcgt tttgttacct gcctctaact
     9781 ttgtagatct ccaaaatata ttcacgttgt aaattgttta acgtcaaatt tcccatacaa
     9841 agctaaggga taatgcgtag cgttcacgta actggaggaa tgaaatggag tttttcaaaa
     9901 agacggcact tgccgcactg gttatgggtt ttagtggtgc agcattggca ttacccaata
     9961 tcaccatttt agcaaccggc gggaccattg ccggtggtgg tgactccgca accaaatcta
    10021 actacacagc gggtaaagtt ggcgtagaaa atctggttaa tgcggtgccg caactgaagg
    10081 acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg ctcccaggac atgaacgata
    10141 atgtctggct gacactggcg aaaaaaatta acaccgactg cgataaaacc gacggcttcg
    10201 tcattactca cggtaccgac acgatggaag aaaccgctta cttcctcgac ctgacggtga
    10261 aatgcgacaa accggtggtg atggtcggtg caatgcgccc gtccacgtct atgagcgcag
    10321 acggtccatt caacctgtat aacgcggtag tgaccgcagc tgataaagcc tccgctaatc
    10381 gtggcgtact ggtggtgatg aacgacaccg tactggacgg tcgcgatgtc accaaaacca
    10441 acaccaccga cgtagcgacc ttcaagtctg ttaactacgg tcctctgggt tacattcaca
    10501 acggtaagat tgactaccaa cgtaccccgg cacgtaagca caccagcgat acgccattcg
    10561 atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt ttataactac gctaacgcat
    10621 ccgatcttcc ggctaaagca ctggtagatg cgggctatga tggcatcgtc agcgctggtg
    10681 tgggtaacgg caacctgtat aaaaccgtat ttgacaccct tgcaaccgcc gcgaaaaacg
    10741 gcactgcagt agtgcgttct tcccgtgtac cgacgggcgc taccactcag gatgctgaag
    10801 tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct gaacccgcaa aaagcgcgcg
    10861 tcctgctgca gctggctctg acgcaaacca aagatccgca gcagatccag cagatcttca
    10921 atcagtacta atcacctcgc cccggtatcg tgccggggct ttttcacttc agacttacgt
    10981 ccattgccaa ttttcattac cctgatgata atcactggaa taaattattc cgcgcgaggg
    11041 ttttcgggtg aaaaagcaat ggattgttgg cacggcgctg cttatgttga tgactggtaa
    11101 tgtctgggca gatggcgaac cgccaactga aaatatctta aaagatcaat tcaaaaagca
    11161 gtatcacggc attctcaagc ttgatgctat caccttaaaa aatcttgatg ctaagggtaa
    11221 tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt gacgatctct atacctgggt
    11281 cggtcagttg gcagattacg aactgctcga acagacctgg acgaaagata aaccggtaaa
    11341 attctcggcg atgttaacca gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta
    11401 ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc gacgatatca aaacgaataa
    11461 taaatatctg atcgtgaata gcgaagattt caattatcgc tttagtcagc ttgagtctgc
    11521 gttgaataac cagaagaact cgattcctgc gctggaaaag gaagtgaaag cgcttgataa
    11581 gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg ggaaaagatg caaacggtaa
    11641 gcaaatgacc cgcgaagacg cctttaaaaa aattcaccaa cagcgtgatg agtttaataa
    11701 acagaatgat agcgaggcgt tcgcggttaa atatgacaaa gaggtttatc aaccggcgat
    11761 tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg tcgattcagc agaagcgaga
    11821 tttcgatatc aacgaacaac ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa
    11881 attgcaggat gactggataa cgctcgagaa agggcaatat ccgttaacga tgaaagtttc
    11941 agaaataaac agtaaaaaag tcgcgatttt gatgaaaatt gacgatatca atcaggctaa
    12001 tgaacgctgg aaaaaagata ccgaacaatt gcggcgcaat ggagtgatta agtaactcat
    12061 gttaagtacc tttgccggat aatataaaaa gttatccggc aaattatttt ttacattagc
    12121 cctagtagct gtggaatgaa caaggatatt tgcgggatgt aagtgatcat catcaacgtg
    12181 attaacaatg cggcataaaa tggcagcaat ggtttgatca aatgttgaat cttcacacct
    12241 gaaatagaac aaccaacaaa tagcgcacta cccaccggcg gtgttaacag cccaatacaa
    12301 aggttggcca ccatcataat accgaaatgt accggatcca ttcccagctc ttgtgcaatc
    12361 ggcaagaaga ttggtgtgaa gattagtaca gccggtgtca tgtccataaa gatgccgaca
    12421 attaataaga cgatattaat cagcagcaat atgaccagtg ggttatcaga aatccccatc
    12481 aacgcgtcgc tgatcatata aggaatatca gcattggtca ttgcccagga catgcccacg
    12541 gaaaaaccga tcaacagtaa tacaatcgaa gtggtaacaa ccgattccag aatcagtttg
    12601 ggtaaatcgc gccattttac ttcccggtaa atcagcaccg agagaataaa tgtgtagaca
    12661 acggcaattg ctgacgcttc agttgcggta aaaatgccgc ccagaatgcc gcccattaca
    12721 atgaacacca gaagcaaact tggtaaggca tccagaaacg ctttacatgc ctgagcaaag
    12781 gttgctcttt ctgaaagtgg gtagccacgg cgtttagcaa taatgccgca gactatcatt
    12841 accgcgagac ccattaagat ccctggcaaa tatcccgcca taaatagcga tgctacagaa
    12901 acgccgccag ctgtcaggga aaacacaatt aacacgtttg agggcggtat cagtaatcca
    12961 gtaatacaag atgagacgtt tacagcggta gaaaatgccg gatcataacc ttctttggtc
    13021 tgaatgggat tcagtgtgcc accaacggca gcagctgccg caacagctga accagaaatg
    13081 gaaccaaaca tcatattcgc caggacgttc acatgaccaa gcgaaccagg aacgcggcca
    13141 accattacct gggcgaggtt aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt
    13201 ccggcaaaga tgaaaaaggg gattgccaat agggcaaagt tatccagtcc attagccagt
    13261 cgctgcgaaa cagtgattgc cgcgatatcc cagggaaaca tcaacagcat tgaagctaca
    13321 gtggcaatac cgattgagaa cgaaatgggc acaccaataa agaccagcac aaaaaaacag
    13381 ccaaacagtg taagcgcgat ataactatcc attaactggc ctcctgcgca ggttgtttca
    13441 gttgttgaat gctttgatta ataaaacaca aggcataaaa catcatcacc aatccactta
    13501 atggcagaat gatatatacg tatcccatcg gtatttgcat cgcggcagat acctgtgatg
    13561 ttgccagtgt tttatcaatt aatttcaggc caccagtcac aataaccgaa ccggcaaaac
    13621 ctaaaataag aacgttaatg actatactaa gaagtaattg cttgcgctta ttcagggcga
    13681 gagcaaataa atcgatagag agatgtctct gcgcgccgac ggtatatgca gcacctaata
    13741 atcctaccca gatcatcaaa aagcgtgcca gctcatcggt taatgtactt ggctggttaa
    13801 gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc attacggcga
    13861 cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat atcattgtaa
    13921 gcatcctgga aaatcagggc cagagactgg cccgaaatat tattgagcag cgttgtcgaa
    13981 tttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca gaggttgaac
    14041 tgcggcacgg aatggggctt tatcaacctt aacgatttca ccacccatag ctttcgcctg
    14101 agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgatcagctt cggatttctt
    14161 cgccgctgtt tcgaggattt gttgctgttc tggggttaat ttattccatg ttttggtgga
    14221 gataaccaaa aagtcaggaa ttgaggcatg ttcatcttca gaaaagactt tggcaatttc
    14281 gatatgtcta gtctgcaccc aggaaggcac gttattttct gcgccatcta ctacaccttg
    14341 ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgagccac ccatcagttc
    14401 aatcattttg atggtggtcg ggcttggctg aacacgaatt ttcagccctt taagatcttc
    14461 tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat aagctgataa
    14521 agcaaaaaag cctttatctt ttgttgaatc cataatttct tcaccaactt caccaaaaac
    14581 cactttgttg aagtgcgcct gatctttaaa taagaacggc aaattataga tagcataaat
    14641 attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg caccattttg
    14701 caacaactcc atagtttctc gcgcattacc catctggctg ctgggataaa tacggatcac
    14761 catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac tctgatgtac
    14821 aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg cggcgtttac
    14881 gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa atgacctcat
    14941 tttcataaaa aaactccctg ttgaagaatg gaaattggtt gatatctaat tgtgcttatc
    15001 agtgctttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt tatcacaact
    15061 tgaaataaag tcagaaatta aatattaatt ttgtgcgcca ggttgtacat atttataatc
    15121 aataaatgat gagtaaatat tcactaaata tatataataa atatcgtcga tatttattca
    15181 gtaagaaata tatttttgcc gaagttaatt aa
//
LOCUS       DAOFSV010000107        15091 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.107, whole genome shotgun sequence.
ACCESSION   DAOFSV010000107 DAOFSV010000000
VERSION     DAOFSV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.107"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1025
                     /locus_tag="QBH27_003398"
     CDS             <1..1025
                     /locus_tag="QBH27_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ7015675.1"
                     /translation="QADAGYGLTLLLNANDDMQDSYNLLPEQELFICNAVIDNMNIYR
                     SQFNKCLRKYDLSEITDIYPNKIILQGIKFDKKKNVYGKDLVSIIMSVFNSEDTIAYS
                     LHSLLNQTYENIEILVCDDCSSDKSLEIIKSIAYSDSRVKVYSSRKNQGPYNIRNELI
                     KKAHGNFITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNGKIQFFYDDKA
                     TRMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRLLQPLILGLWG
                     DSGLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGLLSRYGLFKDV
                     SGIIEQ"
     gene            1067..2245
                     /locus_tag="QBH27_003399"
     CDS             1067..2245
                     /locus_tag="QBH27_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDQ7015676.1"
                     /translation="MFGTLKITVSGAGYVGLSNGILMAQNHEVVAFDTHQKKVDLLND
                     KLSPIEDKEIENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGSNYFDTSSVE
                     AVIRDVTEINPNAIMVVKSTVPVGFTKTIKEHLGINNIIFSPEFLREGRALYDNLHPS
                     RIIIGECSERAERLAVLFQEGAIKQNIPVLFTDSTEAEAIKLFSNTYLAMRVAFFNEL
                     DSYAESFGLNTRQIIDGVCLDPRIGNYYNNPSFGYGGYCLPKDTKQLLANYQSVPNKL
                     ISAIVDANRTRKDFITNVILKHRPQVVGVYRLIMKSGSDNFRDSSILGIIKRIKKKGV
                     KVIIYEPLISGDTFFNSPLERELAIFKGKADIIITNRMSEELNDVVDKVYSRDLFKCD
                     "
     gene            complement(2584..3753)
                     /locus_tag="QBH27_003400"
     CDS             complement(2584..3753)
                     /locus_tag="QBH27_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDQ7015677.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSSYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERILYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAVYY"
     gene            complement(3788..5815)
                     /locus_tag="QBH27_003401"
     CDS             complement(3788..5815)
                     /locus_tag="QBH27_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDQ7015678.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFAALRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERTPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTIADLIYQALIVYPTYI
                     HPTLLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            complement(5812..6552)
                     /gene="kdsB"
                     /locus_tag="QBH27_003402"
     CDS             complement(5812..6552)
                     /gene="kdsB"
                     /locus_tag="QBH27_003402"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDQ7015679.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAAEAAEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            complement(6562..8238)
                     /locus_tag="QBH27_003403"
     CDS             complement(6562..8238)
                     /locus_tag="QBH27_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HDQ7015680.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            complement(8262..9410)
                     /locus_tag="QBH27_003404"
     CDS             complement(8262..9410)
                     /locus_tag="QBH27_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HDQ7015681.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            complement(9482..10465)
                     /locus_tag="QBH27_003405"
     CDS             complement(9482..10465)
                     /locus_tag="QBH27_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDQ7015682.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            complement(11276..11431)
                     /locus_tag="QBH27_003406"
     CDS             complement(11276..11431)
                     /locus_tag="QBH27_003406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015683.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAAGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
     gene            complement(11863..12796)
                     /locus_tag="QBH27_003407"
                     /pseudo
     CDS             complement(11863..12796)
                     /locus_tag="QBH27_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(12789..13184)
                     /locus_tag="QBH27_003408"
     CDS             complement(12789..13184)
                     /locus_tag="QBH27_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDQ7015684.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKSQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
     gene            complement(13253..14098)
                     /locus_tag="QBH27_003409"
     CDS             complement(13253..14098)
                     /locus_tag="QBH27_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDQ7015685.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQHS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            complement(14183..14380)
                     /locus_tag="QBH27_003410"
     CDS             complement(14183..14380)
                     /locus_tag="QBH27_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDQ7015686.1"
                     /translation="MKSLTTETALYILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQLQLLQQNR"
     gene            complement(14397..14885)
                     /locus_tag="QBH27_003411"
     CDS             complement(14397..14885)
                     /locus_tag="QBH27_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015687.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASTECLPDLPTF
                     DW"
     gene            complement(14882..>15091)
                     /locus_tag="QBH27_003412"
     CDS             complement(14882..>15091)
                     /locus_tag="QBH27_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDQ7015688.1"
                     /translation="ISLCDAVNFLVEKYAQVRTDQPGFSAGAPSQLINSIDILRARRA
                     TGLMTRNNYRMVNNITQGKHPEAKR"
BASE COUNT         3953 a         3427 c         3448 g         4263 t
ORIGIN      
        1 atcaagccga tgcagggtat ggcctgactt tattattaaa tgcaaacgat gatatgcaag
       61 atagttataa tctactccct gagcaagaac tttttatttg taatgctgta atagataata
      121 tgaatattta taggagtcaa tttaacaaat gtctacgaaa atacgattta tcagaaataa
      181 ctgatatata cccaaataaa attatattgc aaggaattaa gttcgataag aaaaaaaatg
      241 tttatggaaa agatcttgtt agtataataa tgtcagtatt caattcagaa gatactattg
      301 catactcatt acattcattg ttgaatcaaa catatgaaaa tattgaaatt ctcgtgtgcg
      361 atgattgttc atcggacaaa agccttgaaa taattaagag catagcttat tctgattcaa
      421 gagtgaaagt atatagctca cgaaaaaacc aaggccctta taatataaga aatgagctaa
      481 taaaaaaagc acacggtaat ttcatcacct ttcaagatgc agatgatctt tctcatccgg
      541 agagaataca aagacaagtt gaggttcttc gcaataataa ggctgtaatc tgtatggcta
      601 actggatccg tgttgcgtca aatggaaaaa ttcaattctt ctatgatgat aaagccacaa
      661 gaatgtctgt tgtatcgtca atgataaaaa aagatatttt tgcgacagtt ggtggctata
      721 gacaatcttt aattggtgca gatacggagt tttatgaaac agtaataatg cgttatgggc
      781 gagaaagtat tgtaagatta ctgcagccat tgatattggg gttatgggga gactccggac
      841 ttaccaggaa taaaggaaca gaagctctac ctgatggata tatatcacaa tctcgaagag
      901 aatatagtga tatcgcggca agacaacgag tgttagggaa aagtatcgta agtgataaag
      961 atgtacgtgg tttattatct cgctatggtt tgtttaaaga tgtatcagga ataattgaac
     1021 aatagtttgt tattctatat atattaaatt tttggggcta tataaaatgt tcggaacact
     1081 aaaaataact gtttcaggcg ctggttacgt tgggctttca aatggaattc taatggctca
     1141 aaatcatgaa gtggttgcat ttgataccca tcaaaaaaaa gttgacttac ttaatgataa
     1201 actctctcct atagaggata aggaaattga aaattatctt tcaactaaaa tacttaattt
     1261 tcgcgcaact actaacaaat atgaagccta taaaaatgcc aattacgtta ttattgctac
     1321 accaacgaat tatgacccag gttcaaatta ctttgataca tcaagcgttg aagctgtcat
     1381 tcgtgacgta acggaaatca acccaaacgc aattatggtg gttaaatcta cggtcccagt
     1441 aggtttcaca aaaacaatta aagaacattt aggtattaat aatattatct tctctccaga
     1501 atttttacga gaaggaagag ccctatacga taatctccat ccatctcgca ttattatcgg
     1561 tgaatgttct gaacgggcag aacgtttggc agtgttattt caggaaggag cgattaaaca
     1621 aaatataccc gttttattta cagattctac ggaagcggaa gcgattaagt tattttcaaa
     1681 tacttatttg gctatgcgag ttgcattttt taatgaattg gatagttacg cagaaagttt
     1741 tggtctgaat acgcgtcaga ttattgacgg tgtttgtttg gatccgcgca ttggtaatta
     1801 ctacaataat ccttcttttg gttatggtgg ctactgtttg ccaaaagata ccaagcaatt
     1861 attagccaac tatcagtctg ttccgaataa acttatatct gcaattgttg atgctaaccg
     1921 tacacgtaag gactttatca ctaatgttat tttgaaacat agaccacaag ttgtgggggt
     1981 ttatcgtttg attatgaaaa gtggttcaga taattttaga gattcttcta ttcttggtat
     2041 tataaagcgt atcaagaaaa aaggcgtgaa agtaattatt tatgagccgc ttatttctgg
     2101 agatacattc tttaactcac ctttggaacg ggagctggcg atctttaaag ggaaagctga
     2161 tattattatc actaaccgaa tgtcagagga gttgaacgat gtggtcgaca aagtctatag
     2221 tcgcgatttg tttaaatgtg actaatgtat tgttatatac tattaactat taagagaagg
     2281 aaatgcatta tttaatccgt taaaaatatg cctcgttggt atgttcttta ttaatcctcg
     2341 atcgtaaaat aagatgttgg ttaaatttct tgccctgctg ttgtgtttat atcgaatttt
     2401 tcataactct aatgttaatt agaatacttg gcttgtgcgt tggaaatgta tattatgtgc
     2461 tatatccaaa gatatctaat atatattagt aatatctaaa atactttatc tttttacatc
     2521 taataaaggt atcatccatc tgttatgatt gatataatgg agatgtcgat tgttgataag
     2581 atgttaataa taaaccgcat taacctgcgt cttcaccaat aaatacccac gaaacttctt
     2641 aaacagtttc atatctggtt taaaatcagc ctgccagaac tggtgcaaat gcccttgata
     2701 cgtcaagccc ttgatgtcgt acagggcatt gcccatcact ttgagtggtt tgttatgaat
     2761 cagcgcagag atccccgccg tactgttaat cgtcaccacc gcttttgcat ggcgtaataa
     2821 ttccggcatc gggagatcgt gcacataaag gattcgctca cctaagccat attccttact
     2881 caaccgttta attaatggtc gatagagtct gtgaccacga tccatcggat gatgtttgat
     2941 caccaaataa ctttctttcg gcgctttacg cgaaaatgag tacatgactt cattaatata
     3001 gtcacgcaca tcgttataac tgctgtggtt acgaatctgg ctatcgttat acacctgcaa
     3061 aacagcaaga taataacgct ggtccagttc gttcattaac ctcggtaata ccttacgctg
     3121 tgttaccttg taaagttgct tgcgccagta tgcacgaacc cagcaacgtg cttcatacca
     3181 gggggaaaat gatttgtggt ggcggtagcg aggaaactca tgacggtaat gccagcccat
     3241 caggtaatac cacatagcat ggcctatacg tttcatcgtt gaaggtttta agttctcaac
     3301 gtgcggcgta ggcatatctg gtaacttacg ataaaaatcc ggatcgcgcg gtagcgatga
     3361 atatgcgttc actccgcctt cttcaacggt aataaattgc gggcgtaaat atccttcttc
     3421 aaatgccagg aagcggatcc ctttcgactt tgcccagcgt tttgcttctt tatgcaatgg
     3481 gcggcagtcg ccaaagcaga ggattgtgtc aaagtcatat tgccggtgga gatcccgtaa
     3541 ccatccggga aactctttcg gtgtctggta gtaagctagg tattgtcgat ggcggcagta
     3601 aaaacgatcc ccaccgttga atacaacatt cacagcgtta cgacctaatg actctagcca
     3661 ctcggcaaca tcactgaaaa agggtcccat tggcccctgc aatagcagat attttttacc
     3721 ggataataaa acggttagtg cattaccttg cataaataat ttgtgtaata gtcaactgtg
     3781 aaattgttta gccaaatctg accttgcaga acataattaa tttgcggtaa tagcgtatta
     3841 ctcgtcccgc tttttttcgg gtaataaaca tcggcttgcg cggcgtctgg atcaaatatt
     3901 ccgccgcctc ttcaaccgta ataggttgta gcagtgttgg gtggatatag gttggataaa
     3961 caatcaacgc ctgatagatc aaatctgcta tcgttaattt ttgctcgcga cgcgggcagt
     4021 gatgttcatc aacggttaaa ccccaaccgg catagaaggg caggccgtaa caatgaactt
     4081 gcttgccatg taataacgct tcaaacccgg acaatgatgt catggtgtgc acttcatctg
     4141 cgcgctgaat gcattgaata atatctgcgt ccagtgcctg ataatcagcg agttcagcaa
     4201 ttagttctgt cggaatattg cccttgcgat tccccaccag tacatccggg tgcggtttat
     4261 aaataatgta agcgtgcgga gtgcgctcgc gtactgtgcg taataactca aggttgctct
     4321 taatcgacac agtgcctgtt ttaattgagg catcgtcttc tacctgaccc ggcaccagga
     4381 tgattttttt atctttggcc tcggcaggca gagagaaatc ggcccccagg ttgtatttgc
     4441 tcagtttgct ttcaaccagt cgctggcgta atttttcagc tcgcatctgc tgcgccagtg
     4501 ttaactggct gtgattaagc agcacttcca ggtcgctggg gcgcgtggcg tcatagtaga
     4561 tcccgcgttt atccaggacc aacgatagcg gcggcaaaag gtcagagcca agtccggatg
     4621 aacgtagaaa accatcttcc attcgccata acggcagtga ttttcgctgc gcttcggctc
     4681 gccattgctg ttctcccttt acaccccata ccacgcaggc gctcgccgca gtacagcgac
     4741 gtgaaaaact cagccggttt gttgccgttt gcaagaaagg tttcaggatc gctgacttcc
     4801 acagcgttaa gcctggcgcc cataaataac cattacgctg ttgcagatgt cgacgttgta
     4861 attgcagcca ttgcagcacg gtaaatagat tgcttacttc tcccgtttgc ggatcgatat
     4921 agcgacagta acgcaggtat gcagcggcaa aaagttcctc cagcgtggca gaaccgcgtc
     4981 gggcagataa caaagcggac tgcggatggc gatcgtcggt taagccccag cctgcatacc
     5041 agggctggcc gaagcatgtc accggttttc ctgccagcaa ggcttcaaag ccgtactggg
     5101 aggtcacgac gtaaacccgg gaaacgtgtc gcaacagcga ctgcgggctg acattctcgg
     5161 caattaaacg tactcgttgc gtggcgcgca gagcggcgaa ataacctgtt ttctttcctt
     5221 ccaggacatc cggatgcacc ttcacccaaa tttcggcttg aggattttcc gccatcgcgg
     5281 cttccagcat ggcagcaaac tcatgcgggc cagcattgcc atacgtcact gacatatcat
     5341 taaatgtctg atcgacaacc agaacgatgt ctgaacgttc tgactcatca gccacaaacg
     5401 caggtgccag gttatatttc gacaaatccc cggtcacgat ggtgtgcatc gcttctctgg
     5461 cctggcttat cagagctgta tttccggctt tatcttgtac cagtttctcc agtgctgaag
     5521 gcttgctggc atcgtagtaa atgcaacaat catccaccac cagagaaagc ggcggctcgc
     5581 cattgacgcc aagatccagc gaacgcacaa atccatcttc cagacgaatg acgggttttc
     5641 ccgctgcttt ggcgatggcg actggtttcg ccgcgctggg acgatgtccc cacacggcga
     5701 tagcatcaac ttcttgtgga acagggcgca gcagagaaag tttctggcac ggttgcgcca
     5761 gaaatttctc cagatgcgga atacgccaga tgccaggcga gtaaatgcca atcatgcgtt
     5821 ttcagccagt tcctgtgcca tcagggcgcg cactttttcc aggcatgctg gggtgtcgac
     5881 gcccggaccg gttgcggcaa cctcaaatgt gcggatgttg atccccgcgt tcatcaacct
     5941 tagctgctcc agtgattccg cctgctccgg catggactcc ggtaactggc tgtagttttg
     6001 cagcacatcg cgacgataag cgtaaatacc gacgtgtttc aggtagcgcg ctttttcagc
     6061 attacgcgga tacggaatcg gtgagcggct gaaataaagc gcatcctggc gggtattcac
     6121 aaccactttt accgtgcttg gctcagccgc ttctgcggca gaaatcgcgt ggcatagcgt
     6181 tgccaccggc aacgcgggat cgtcacgcat tccttgtagc agcgtttcta catcccgcgg
     6241 gcgaatcatt ggttcgtcgc cctgcaggtt aatgtagata tctgcctcga ctttatgcat
     6301 cacctcgacc agccgatcgg tgccggattc atgatcgttg cgcgtcatga tggctttccc
     6361 gccaaacgcc tgtacggcct gttcaacacg cggatcgtct gttgccaccc aaacttccgc
     6421 aacgcccgcc acctgtaacg cacgctcgta aacatgctgg atcatcggtt taccaacaat
     6481 atcgagcagt ggcttacccg gcaggcgcga ggagccatag cgagccggaa tgacaataac
     6541 tgctttgctc atttcaaccc cttacaaaga cagaatcact tttgcaccca ccgccagctg
     6601 atagaggatg gtggaaatac cacgggtaac ttcaatgttt ttcgattcat atttcggcag
     6661 aaccataatc tcatcgccag gcttgagtga atctacatct tcagcgttga ctgccgcacc
     6721 gttctgacga atgacgataa ttctggcgtt acccgatttt tgcgtcaggc caccgcattt
     6781 ctcgatgtaa tcctcggtgg tcatcccttt ctgccagctc accgcgttcg ggaacagcac
     6841 ctcgccatga accataacca gcgatgtttt ctccggaata ttgatgacgt cgccatcttc
     6901 aagcagaaca gaatcaatat tggattcgtt gaggatcact tcacctttcg gaaccacggt
     6961 gcgtgctttc gccacaaagc ggctgatcag ttgtgcttcc tgcattcgca ggctggcttc
     7021 ttctttggtg gatgactggg cagaaagtga ggcttcctcc agcttttgca gcgagagatt
     7081 cagcatctct ttctgacgct gagccactga tgggcgataa agctgaactg cgttcatctg
     7141 cgacatgctg ttcgggcgga ctttttccag aaccgcacgc atagtggaac cataaggcaa
     7201 taccatggca tgttcaccgg agtgtgcgcc ttcaacccgc acctgaatgg tgccggcata
     7261 gcggtcagtg ctcacgatta aggtatcgcc attttgcaac atacggcctg gcgcagaact
     7321 gatcggatag tattcgctgc gtttttgcag cccctgttta cgcataatcg taatgtgagt
     7381 cgcgccaggc ttagggcgcg cccagctcaa cgcttccgtt acgggaatgc tgctttcgcg
     7441 gaactcaaag tcgtagctgt taaagacatc gccctgaacg ctgaaagtat gctgacgtgg
     7501 cccgacgatg atggtgtcac catcggcgaa ctgcgaaagt cccagtttgc cgttcagcag
     7561 gaagtcgtac aggttgacgt tggagcgcac gcggttaccg cgcttgacca caatatcaac
     7621 gtaacttccg cgctctggat caacgccgcc agccttgatc agataattga gtaacgaatc
     7681 agacgtcaca ccgccataca gaccaggatt acgcacaaat ccggtcacgt acacttttac
     7741 tggctgcgcc tgtaataagg aggcgtagac gttgacgttg gactggtata cttccttcac
     7801 tttggatgtg accaaggcat ttagctgact attactgacg ccagcaactt tcaccggacc
     7861 aacgttcggc aggaaaatat tacctttggg atcaacctgt aacgcaccat caaaggtgaa
     7921 cgcaccccac aagcgaacct gaatgctatc acccggattc agaatatagt cagggttgaa
     7981 tcctaccgtc gcaccgctat ccgcgctggt gccgttgaaa agttgagcac caaacatacg
     8041 gctcattacc accggcggtg cgggcggcgg ggtattgtcg aagccgctca tgttttgcgt
     8101 atccgacttc tgtccgttca gaataccggt aagcggcgcg gctcctgtca ggtttgggtc
     8161 agcgttaata tcaatggtcg cgctggcctg cgccgcgtga caggcggcaa tcagtaaaat
     8221 tgatttaaat aatttcatca caaactcatt cagcgacagc gttagtctcg gtgatcttca
     8281 ataacagcca gcaacagttt cagggtgccg aacagcaggc agcacaccag taaccagcag
     8341 gcgatcaaat aagggatatt tggaaaagac gattcctgcg gcaactgtgg cgaactgatc
     8401 actgacagca ccttgagctt acgagccgct tctacacggg tcttttcaat ggaggtcagg
     8461 gtcagtttgt acagctcggt gttgaactct acttttgatt tgatttcttc aaaatccact
     8521 gccatacggt ttagcttgtc accctgtggc gcagtgattt tgcttttttc ttcgtcaatt
     8581 tgtgcctgca atgactgaat cgcattacgc gcactcacaa cttgcggggc gtcctcacgc
     8641 agatacgtca gcaagttccg caggtccgct tccatctgga tcttctggcc catcagcgta
     8701 ttcactaacg tgctcgccgc ctgtgcctgt gcctgtggat ccagaacgtt gttgttgtcc
     8761 tgataagaga gcaattccgc tttgctggcg tccagacgct ggcgtgcctt ttccatctcc
     8821 gtttctgcaa aggcaagctg gtcacgcgcg atgcgatgtg acatctcatt gataaagcgc
     8881 tctgactctt tcagcacggt ttggttaaac ttaagcgcaa actccgggct aaagccctgc
     8941 gtctgaatat tcagtaatcc ggttttatcg tcatacgaga cgttgatacg gtccttgtag
     9001 tacttcagga agccttctgc ggtttcatcc ttgctaagat gattaagaaa atcgagcccg
     9061 ctgtggctaa acgcttcacg aaaatttagt tgcttatcca gcgccgccag catatccggc
     9121 gagttgatgt actctttcag atacaacgca tcttctgcgg aactggggtt agaggcaccc
     9181 agaagcagac caaaattcag gctgccgctg tttaaatcat ccgagcgttt aatggcgact
     9241 ttcgactcgc tcatatagcg aggctggctg aagatagcca gatagatcag cagcaccgcc
     9301 atcggtgcca gaatgatgat tttcgccagg tgtttttgga tatctgccag tgagatggca
     9361 gacagacgag cacgcatcca ggatacggca gacttcactt ttatcaacat gtaacgatta
     9421 tcaaagtttc agtggggcgc aaactcaatg agagactaac ctctcgcacc ccgttgctta
     9481 attagtcgaa aatgcgcaca aggccagtga ctttatttgc cttgttggtc accaataacg
     9541 ttgagacgcg gtgcttttgc attttttctt ccgcttcaat gatcatggtg tcttccggta
     9601 gcgtcagcgg ttcacgtgtc atcatctgcg cagccgtggc ggatgtcaga gaatcctctt
     9661 tttccataaa gcgacgcagg tcaccgtcgg tgataatgcc cgctagccca ccttctgcgt
     9721 cttctaccat caccattccc tggcatccgc tggtgatacg ttgaatcacg gttttaaatg
     9781 acgcatccag ctgtaccgca ggaacatcat gctgcatgac atcagcaacg cgggtcagca
     9841 ggcgacgacc taatgaaccg cccgggtgat agcgcgcaaa atcattcggc ataaatttgc
     9901 gttggtggat catggcaatc gccagcgcat cgccgatcgc catcgtcagc gtggtagacg
     9961 ttgttggtgc aagattattc gggcaggttt cattcgccat gtggagttcc agcacggcat
    10021 cagcattttt cgccagcgtg gaatttccat tattggtaat ggcgataatt cggttgccga
    10081 aatttttcag tgaaggaact agcttgagga tttcatccgt ttcaccgctg gcagaaataa
    10141 ggatcagaag atcgtaaggc gtaatcatgc ccagatcgcc atggaaagct tctgcaggat
    10201 gaataaagaa actaggcgta cccgtagagg ccagcgtcgc tgacatttta cgaccgacat
    10261 gccctgattt acccattccc gaaagaataa cgtgcccttt acagttcatt atcaaattaa
    10321 ggacacgctg gtactgcccg gaatccagtt gtttagacaa gttttgtaat gctgcgcttt
    10381 gttctgccag agtctggcga acagaggtaa ttagatatgg atcgatagta ctgctctggt
    10441 catcaggtaa atgtctttca gacatatcag ctcctttgca cggaaataat gccacagtca
    10501 caacaaaccc gaacccacta taatttattt atgctaaatc aatgggttgt aacaaaattt
    10561 ttaatgaata taaaaccatg gtcagtccat gcttatatgc aggacttatc accttcttat
    10621 taagtcgcaa tgtctcatgg aggtgcgaag acgctgcaat attacaggcg agaaagggaa
    10681 ccaaattttg cccacctatt taacacactc ctacattatg aacaaggaat ttaggatttt
    10741 tatcatttgt taaaatgttt cctacttgac tattaatacc aaaaacaatt tatcaattga
    10801 ttatttcctt gtaaaaaaag aacgtatgac tatgttatta ataaataaaa cacgcaaata
    10861 atggtgatgt tgttttgtga ttaattattt aaattcggca taataaacag gttgataatt
    10921 tctatgtaaa gaagaggaaa ataataaaat tttagcggtg actattgaat gatataatag
    10981 ttgtagtgtt gattatttct ggtaatgaat gcggaattaa atctggccgg attgtatttt
    11041 tgctatatct atttttcagg agattattat taaatagaaa aataacaatg gtgattatct
    11101 ccggttgagc tggagtcacg tatgccagcg agtgctttct tccgggcctt ccttcacttc
    11161 agagaaagag cctatggaaa agaggcaatc atcaggctct ttttgagtca tatgcgccga
    11221 taatcaactg tcttgaacgt aaaaattatg gagcaacatg atgctacgat tcatttcact
    11281 cttcgttctt tctgagcatt gcgttcagga tggtcagcaa tttacgcata cagtcaacca
    11341 gcgccacttt tttggttttt ccggctgctg gcagatgcat gtaaaacgcc tctttcaccg
    11401 gattaaacca ggttaccaca agcgctgcca tgtgcagggc tgctcttgcc tccgtacgcg
    11461 cactggcgtc gttcggccag cagatatgtg tgggataaca gggatgttat gtgaactgaa
    11521 ctgtgttttg ttagcggggg atgtttattg atgattcggg agtgtttaag acctgatggc
    11581 gttatgcacc caagtttatt ttatataaag atccaaaagc aaaaacccgc cttgtgggcg
    11641 ggttcttaag aattagtggt gcccggaatc ggaatgaaac tgattgtata acaaattgtt
    11701 ttatatagat tattttgtat ttgttgtttt tgaggccacc aatgaggccc ccatgtgcaa
    11761 ttcgaattct tttgacgcta taatccctct tttttgtgct ttgctgtaat catttggtct
    11821 ggttcgatat gctgaattgt ggaatcgtac ttaacgcgtt tgttagctgg tcagaacact
    11881 tagcgctgtt tgtctgaaac tggaataggc ttcttcccag ttaatctgtg tattaccaaa
    11941 taccatcggt ttaacaaatc gttgaaagcg caatttgtgt acaggataat cccggagtgc
    12001 atccagccct ttcttaagtt catcaactgg tgagctacaa aattgtgggt actggttacc
    12061 atagctgaat acatatctga acaaaatttt tcaatacagg aacattcaca cctttttccc
    12121 tgacaatgca gtagttgtcg taaatgtgac gtaccagaaa ctcatcatcc tgttgttcgg
    12181 gattacgaag atgtgctgct gttcttcgca tcatggagat gagcttttca gcctgagtgg
    12241 ataaaatggt ggcacagggg aatgctgtta caggtgtacc tttacccgtt aactccgtga
    12301 caagggaata aatatctctg ctttccggat gttccagcag agtgctttcc atcaactcca
    12361 gcttaataaa tggacgaaga caggggactt gtgcaaatgt ctggggataa cgtaccgaga
    12421 tttcgttata acgatactca tcacgtgtta tacgggcgtt ttcattatcc aagctgaaaa
    12481 tgtcagaatt atgaataaca tccgtgatta tttgcacgat ttccttgcga atttttcttc
    12541 tcttgtctct gccattttgc ataaagttct ctgttggcac gagctttata tcaatatctt
    12601 ctgacattct gtttagcgag atacctgctt ttgataatgc ggtacctcct gcaaacacga
    12661 gagtatgtgt ttctggtttt atttctttta ataatcttaa aagatcaacc acaaaataat
    12721 ctttttcgac gatagcgaca gattcaatat ccagtgcatc ggcaacgtca ggaaataatg
    12781 atgttaattc aggcattgat tttacttttc cctactatta acattctttt aaatcgagaa
    12841 ggtgttttta tccccagaga tacaggaatt tgtgtggact gacttttatt gtagcattcc
    12901 gttgctcctg ccagacggta tctgatattc aggcgctcca gggcctctgt aattactgca
    12961 gcagagcccc caggcgctgc gggcatgatt ttcccagtga tgatgttacg acgggctttg
    13021 gtgtaaacac catagcccac tttcatcaac ttaccttcgt ttaccagtgt acgaagagct
    13081 ctgccaacct gatcatagcc agcgatatcc ctgaaatcat cccgggtgaa cacataacgt
    13141 tttgaacggt tcagtcggga ctgaatacgg tcttttgtag tcatgtttcc tccgaaaaat
    13201 tttcacaatg atagcaaaaa tatgacatgt ttaatagcgg ggcaaggcca cattacctgt
    13261 ctgctaatac atttggatag tgcctggcaa ttatgtcatt tagcctgtca accagttctg
    13321 gtttcctgaa cgtgatgtgt gcggagccct tcttaaagta tctgatgctg aacatctcat
    13381 cttcatagca cgctttgccc tgaacggagc ggatgtgatc atccagccga atgatgatat
    13441 tttcccggct gtcggggatc ggtttgccgc tgaacagatg cagcattctt tccaggtcag
    13501 cgagtcgatc tgcctgttgc ccggtgataa gatgcaatcc ccatcggtcc catctcacca
    13561 gattgttgac gataatttta ttgccaaatt tacaggggca attggttttg taattccagc
    13621 tcagccctct gaagaggttg atcacacctc gctcaaacac gtcatcctta ttctggtgca
    13681 gttgttcaaa cgtactcagg atattcgcct cgctgatttc cggaaagtta tcgtactcca
    13741 gtgagcggta ccatgtatca tgggcctgcg catccattaa tgacagcatc ccggaatgtt
    13801 gcatcaggtc acgccagatt tcgcgatcga gattgcgggt gatggctttc attgcggttt
    13861 ccggtttctc catgagccag cagccgcagc gaaaatcctg tttcatcgcc cagtccaggg
    13921 cggttttacc tccaatactt tctgtcagta ctgagatttc tgccagttgc cggataagtg
    13981 tgtcgatttg cagcagcgca ctctttcgta cagccagaat gtggcggata ctggttgagc
    14041 agataacgtc cgtatgtccg ttgaccagtt ccgggcggga gattgtgata tctgacatca
    14101 tcgtttttcc ttttggttta taaacgaaaa cgccagccgt attcaggctg gcgtcagtgg
    14161 aatgaagtct ggtgagtaag actcaccggt tctgttgcag aagctgcagt tggcgcaggg
    14221 tacggatatc ttccctggcc tgtttaatgc agggcagcag tgctgctgaa tccgttttat
    14281 cctcatcgtt gtcgaagata atccctgatt cgcagtcaat attgtcctgc agccacgcaa
    14341 tcagaatata cagcgctgtt tccgtggtta atgatttcat gttgtgaatt tccgggttac
    14401 cagtcgaaag tgggtaaatc cggcaggcat tcagtgctgg catccagatg aatgagactg
    14461 acgccataac gtcgggtaag tgtgacaacc agacggcgga acgtttttga cagccccagg
    14521 cgtttaagcc gcagaaccgg gtatgaccac gcatcggtac gtaacagata tccgctacct
    14581 gtaaaatgaa tccattctga ttcgccggtg tcattcgtct gatgcgacag cgagtacagc
    14641 caggcgttgt ctgtttccgt gatatgcgcg gtactacagc gtataccgct cagggcagca
    14701 gaggggggta tgctgtctgt tgaggtccgg tcagattcat caacaacacg aagggagtag
    14761 ccattatcgc agaccacgtt aatcagttcg gcaagctcaa caccatcgac gtcaacgaca
    14821 attcgcccca tgttcagtgc ctgtacgtta acgctgtcag cttccagcgt cagggccagt
    14881 ttcatcgttt tgcctccgga tgcttgccct gggtaatgtt atttaccatt ctgtaattgt
    14941 tccgggtcat caggccggtc gccctgcgag cccggagaat atcaatgctg ttgattaact
    15001 gcgacggggc tcctgcgctg aatcccggct ggtcggtacg tacctgcgcg tatttttcaa
    15061 cgagaaagtt caccgcgtca cacagtgaaa t
//
LOCUS       DAOFSV010000108        15016 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.108, whole genome shotgun sequence.
ACCESSION   DAOFSV010000108 DAOFSV010000000
VERSION     DAOFSV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.108"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..809)
                     /gene="fkpA"
                     /locus_tag="QBH27_003413"
     CDS             complement(<1..809)
                     /gene="fkpA"
                     /locus_tag="QBH27_003413"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ7015689.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAK"
     gene            1030..1248
                     /locus_tag="QBH27_003414"
     CDS             1030..1248
                     /locus_tag="QBH27_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDQ7015690.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(1297..1887)
                     /gene="slyD"
                     /locus_tag="QBH27_003415"
     CDS             complement(1297..1887)
                     /gene="slyD"
                     /locus_tag="QBH27_003415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDQ7015691.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(1982..2182)
                     /locus_tag="QBH27_003416"
     CDS             complement(1982..2182)
                     /locus_tag="QBH27_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDQ7015692.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(2192..3997)
                     /gene="kefB"
                     /locus_tag="QBH27_003417"
     CDS             complement(2192..3997)
                     /gene="kefB"
                     /locus_tag="QBH27_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDQ7015693.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSSASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(3997..4548)
                     /gene="kefG"
                     /locus_tag="QBH27_003418"
     CDS             complement(3997..4548)
                     /gene="kefG"
                     /locus_tag="QBH27_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDQ7015694.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            4679..6592
                     /locus_tag="QBH27_003419"
     CDS             4679..6592
                     /locus_tag="QBH27_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015695.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGQAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            6592..7614
                     /locus_tag="QBH27_003420"
     CDS             6592..7614
                     /locus_tag="QBH27_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ7015696.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWHRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            7608..7826
                     /locus_tag="QBH27_003421"
     CDS             7608..7826
                     /locus_tag="QBH27_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDQ7015697.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            7880..8749
                     /locus_tag="QBH27_003422"
     CDS             7880..8749
                     /locus_tag="QBH27_003422"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDQ7015698.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(8804..9208)
                     /locus_tag="QBH27_003423"
     CDS             complement(8804..9208)
                     /locus_tag="QBH27_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDQ7015699.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            9510..10142
                     /gene="crp"
                     /locus_tag="QBH27_003424"
     CDS             9510..10142
                     /gene="crp"
                     /locus_tag="QBH27_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDQ7015700.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            10193..12283
                     /locus_tag="QBH27_003425"
     CDS             10193..12283
                     /locus_tag="QBH27_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ7015701.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12350..13570)
                     /gene="argD/dapC"
                     /locus_tag="QBH27_003426"
     CDS             complement(12350..13570)
                     /gene="argD/dapC"
                     /locus_tag="QBH27_003426"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDQ7015702.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(13656..14219)
                     /gene="pabA"
                     /locus_tag="QBH27_003427"
     CDS             complement(13656..14219)
                     /gene="pabA"
                     /locus_tag="QBH27_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDQ7015703.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(14251..14853)
                     /locus_tag="QBH27_003428"
     CDS             complement(14251..14853)
                     /locus_tag="QBH27_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDQ7015704.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNKLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYLEKEGNAL
                     MQDLEEEKYLTSLEKDKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEVGESE"
     gene            complement(14843..15010)
                     /locus_tag="QBH27_003429"
     CDS             complement(14843..15010)
                     /locus_tag="QBH27_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDQ7015705.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
BASE COUNT         3682 a         4184 c         3737 g         3413 t
ORIGIN      
        1 tttttagcag agtctgcggc tttcgcatca gcttccggct ttgcatcagc cttcggcgct
       61 ggtttcacat ccagcagctc tacgtcaaac accagggtag aattcggtgg gatccccgga
      121 acacccgctt tgccgtaagc cagttctggt ggaataacca gtttgatctt accgcctttc
      181 ttgatgttct tcagaccttc tgtccaaccc ggaataacac cgtccagacg gaaagagagc
      241 ggttcaccac gggtgtaaga gttgtcgaac tctttaccgt cgatcagcgt acctttgtag
      301 ttcactacaa cagtatcgct gtctttcggt gcttcgcctt taccggcttc tactacctga
      361 taaaccagac cagtggaaga ggttttcaca cctttctctt tggcaaattt ctcgcggtac
      421 tctttacctt ttgcttcgtt atcagccgcg tctttttcca tcttcgcctg agcagaagac
      481 ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct cttggtcgga aagtttgctc
      541 ttatcagcaa atgcatcctg aacaccagcg atcagctgat ctttatccag tttgatgccc
      601 agtttttctt gttctttaag agagttttcc atgtaacgac ccagcgaagc acccagtgca
      661 taagctgatt tctgatcgtc atttttgaac gctgctttgc tgtcagcagt tgtagcaggt
      721 tttgcagctt cagcagcaaa agtgattggt gcatgcaggg caacggccat tgtggtcgcc
      781 agcagcgtta ctttaaacag tgatttcatc catatctcca ggatcggggc atctcacccc
      841 agggttaact attatcagaa gggtactata aagcgttgtc gaacaaatct acatacagac
      901 acgccctatt atcatctatt ttcagactct ttttgtttaa attagtttcg atgaccgcga
      961 aatgagtgct gtctcgggca gcaaagttaa gtagaatccg cggcgaccat tcgacaaaag
     1021 aggtgaatca tgcaggattt atcattggaa gcacgcctgg cagagctgga gagccgactg
     1081 gcttttcagg aaatcaccat tgaagaactg aacgtcacgg tgaccgctca tgaaatggag
     1141 atggcgaaac tgcgcgatca tctgcgtctg ctgaccgaga agttaaaagc cagccagccg
     1201 tcgaacatcg cgtcgcaggc tgaagaaacg cctccgccac attattgagg cgtaaaaaaa
     1261 gcgggaattc cccgcttttt tgtcaccttt tcggtattag tggcaaccgc aaccgccgtt
     1321 gcctttaccg ccacagcagc cttcgccacc gtgttcatga ccgtgatcgt ggccatgacc
     1381 accgcagcaa ccgtcgtggt cgtgatcgtg gtggtgatcg tgcgcgccgt gaacgtgacc
     1441 atgagccagt tcttcttcgg tcgcttcgcg aatcgccaca acttcaacgt tgaatttcag
     1501 gttctgacca gccagcatgt ggttaccatc aaccactacg tgatcgtctt caaccgcagt
     1561 gatttcaacc ggtaccggac cctggtcggt ttcagccagg aaacgcatac ctacctgcag
     1621 ttcatcaacg cccataaata cgtctttagg aacgcgctgc accaggtttt cgtcgtactg
     1681 accgtaagcg tcattcgcgc caacagcgac atcaaatttg tcgccaactt catgaccttc
     1741 cagcgccgtt tccaggccag agatcaggga accgtgacca tgcaggtagt ccagcggcgc
     1801 actcaccgga gactcatcaa ccaacacacc gtcttctgta cgtacctgat aggccaggct
     1861 gaccaccagg tcttttgcta ctttcatgat atctcctgag catgggaaga atagtggcgc
     1921 agattgtagc ggaattctgc agccgtgtac tcactagctt aaaaaaactc ggcgcatatc
     1981 gctagtccgg atgaaaaatc ccgatcactt gctcatcttt gcgaacgtga tcgcgggctt
     2041 ctttgtctgc ttctcgcatc tgatgtccac acttaacaca ttcaacgata tcaatattat
     2101 tttcgcgcca catcgccatt gaatcctgag cctgacaggc cgggcatttt gcgcccgcaa
     2161 taaaacgttt tcggattgcc atctttaccc tctactcaaa ttcatcccag ccgtccagct
     2221 ggcgtcgttc ttgttgcatt tcacgctgga aaatctcttc cagttcgcgt cgggcttccc
     2281 tggcgcgaga aatttgtacg gtatcagcat gcatcgggat taactcccgc agcattcgca
     2341 tatccaggcg gcgaaaatgc agttgcgcgc gctgcgcctg atgcggatgc atgccaagcg
     2401 tgaccagcgt cttgcgcccc agctctaacg cactggagaa tgtttcacgg gaaaactgcg
     2461 tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc gcgcgctcgc gcaagaatat
     2521 gcaaatgcgg aaagtgctgc tggcatattt ccaccagctt catagtgtct tccggctcgt
     2581 tacaggtaat gacgatagac tcagcggcct ctgcacccgc agaacgtaaa agatctacct
     2641 gcgtggcgtc gccgtaataa actttgtagc catatttgcg catcaggtta acggcactga
     2701 tatcccgctc cagtacagta atgcgcattt tatttgccat cagcaaacga ccaatcacct
     2761 gaccaaaacg cccaaagccc acgacaataa cctgcggttt atcatcgttg acccatggtt
     2821 tttcgtcttc ttcttccggt ccgttaaact ggcgggatag ccatttatct accagcttca
     2881 tcagcagcgg cgtggtcatc atggaaagcg tcaccgtcac cagcaacagc gccatctggt
     2941 cgccctggaa taagcgttgt gaagaagcgg aagaaaagag gacaaaggca aactcaccgc
     3001 cctgactcaa cacgccagca aactgcatcc gctctgagct acgcacgcca tacaatcgcg
     3061 ccagcagata cagcacgaga attttcaccg ccaccagcac aaccacactt atcactaccc
     3121 acaacagatg ggtataaagc accccgaggt tgagtgacat gccgacagag ataaagaaca
     3181 aaccgagcag caagcctttg aagggatcga tagccgtttc cagttcatgg cgatattcac
     3241 tttccgccag tagcacaccc gcaataaacg taccgagcgc catcgacaac cccagcgcat
     3301 ccataaacaa tgcggaaccc aacaccagca gcagcgtcgc ggcggtgaac acttcccgca
     3361 caccagaagc tgcaataaaa cggaataccg gacgcagtaa atagcgcccg ccaatcagca
     3421 tgccgacaaa cgccagcacc ttcatgccga tcttcatcca gtcgaaatgt tcgtctgccg
     3481 aacccgccaa caacggcact aacgccagtg ccgggatcac cgccagatcc tgaaacagca
     3541 gtaccgagaa acctaactgg ccggattcgc tgcgattcat ccctttttca cgcatcaact
     3601 gcaatgccat tgcggttgaa gacatcgcaa ggccaatgcc accgaccacc gccgcctgcc
     3661 aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc cgcgcttaac agcacctgtg
     3721 ccgcgcctac gccaaaaatc gaacgccgca gttgccaaag cttggaggga ttcaactcaa
     3781 ggccgatgat aaacatcagg aataccacgc cgagttccga aaagtggagg atctcatcga
     3841 cgtcgctaat aaaccccagc ccccacgggc caatcgcaat ccccgccagc aaatatccca
     3901 acactgcacc aatacccagc cgcgatgcca gcggcaccgc agccaccgcc gcgaagagaa
     3961 acagcactcc tgcgagtaaa aaatcagaac cttccatcag cggcctcctg gagacaacgg
     4021 atttgccagc cagtcaccgt aggctctggc gtggctcgcc agctcctgtg cgctttgccg
     4081 tctcgcccag taaataatga tggggcttaa ccaatgcatc cggcacatgc ccgccgccag
     4141 ttcaaagggg cgcagaacat cgctcatcgg atagcgattc aacgcgtcat aacggtaagc
     4201 actttccggc tccccggtgg taatcacgct acgccagtac tttcccgcca gttggtttcc
     4261 ccccggtccg ctggcaaaac cacggcttaa tacccggtcc agccactctt tcagtagcgc
     4321 cgggcagcta taggtataaa gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa
     4381 tgcctgctca cgagggatat caataaaaaa gtcgggatag tgcgcgtaaa ggtcgtgcac
     4441 ggtaacattg ctgagctgcg tggccggttt aagcagaacc cggtttgcca ccgagtcctg
     4501 agattccgga tgggcataca gcagcaaaac tttcgctggc tgagacatca tccccctccc
     4561 ggtatggttt ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt gcggcagctc
     4621 gcccatacat tacattatca taatgataag ttaacatagt ctgaacatac ggcgccttat
     4681 gattgttttt tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac
     4741 cgccaccatc aaccctgggc agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc
     4801 taccctgctg gcattgctga aaaatgaaat cagcgccgac ggcggcagct acacctttcc
     4861 gggaagctgg caactggcgt gggtgaatca ggaaacaccg gcgttaccgc aagcggcgct
     4921 ggaatatgtc attgacggcg accgtgaata tcgtcaactg gaagcgcagc tacacgacgc
     4981 caacgaacgt aacgacgggc aagccattgc aaccattcat ggcaagctgg atgctattga
     5041 cgcatggagt attcgctccc gtgccgccag cctgctgcac ggcctcggtt tcagcaatga
     5101 acaactggag cgcccggtaa gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc
     5161 ccaggcgctg atttgccgtt cagacttgct gctgctcgac gaaccgacta accacctcga
     5221 tctcgatgcc gttatctggc tggaaaaatg gctgaagagc tatcagggca cgctgatcct
     5281 gatctctcac gaccgcgact tcctcgatcc gatcgttgat aaaattattc atatcgaaca
     5341 acaaagcatg ttcgagtaca ccggcaacta cagttcgttt gaagtacagc gcgccacccg
     5401 tctggcgcag caacaagcga tgtacgaaag ccagcaggaa cgcgtagcgc atctgcaaag
     5461 ttatatcgac cgtttccgtg ccaaagccac caaagcgaag caggcccaga gccgtattaa
     5521 gatgctggag cgtatggagc tgattgcccc ggcgcacgtc gacaacccgt tccgctttag
     5581 cttccgcgcg ccggaaagcc tgcctaatcc gttattaaag atggaaaaag tcagcgcagg
     5641 ctatggtgat cgcattattc tcgactcgat taaactgaat ctggtccccg gctcgcgcat
     5701 tggtctgcta ggccgcaacg gcgcgggtaa atcgacatta atcaaactgt tagccggtga
     5761 acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg atcaagctcg gctacttcgc
     5821 ccagcatcaa cttgaatacc tgcgcgccga cgaatcgcct attcaacatc tggcacgttt
     5881 agcgccgcag gagctggaac aaaaactgcg tgattacctc ggcggctttg gtttccaggg
     5941 cgataaagta accgaagaaa cgcgccgctt ttccggtggg gaaaaagccc gcctggtgct
     6001 ggcattaatt gtctggcagc gtccgaatct gctgctgctc gacgaaccga ctaaccacct
     6061 tgacctcgac atgcgtcagg cactcactga agcattaatc gagttcgaag gcgcgctggt
     6121 tgtcgtctcg cacgaccgtc atttgctgcg ttccaccact gacgatctct acctggttca
     6181 cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat tatcaacagt ggttgagcga
     6241 cgtacaaaag caggaaaacc aggccgacga agcgccaaaa gagaacgcga acagcgccca
     6301 ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc agccactgcg
     6361 taaagagatt gcccgtctgg aaaaagagat ggagaagctg aacgcgcaac tggcgcaggc
     6421 ggaagagaaa ctcggcgaca gcgaactgta tgaccagagc cgtaaagcgg agttgaccgc
     6481 ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct
     6541 ggaagcccag gagcagcttg agcagatgct gctggaaggc caaagcaact gatggcgcag
     6601 ataacgacga ccgatgccaa tgaatttagc agcagtgctg aattcacccc tatgcgcggc
     6661 tttagcaatt gtcatctgca aaccatgctg ccgcgtctgt ttcgtcgcaa ggtgaaattc
     6721 accccgcact ggcatcgact ggagttgccc gacggcgatt ttgtcgatct cgcatggagt
     6781 gaagatcctg cacaggcgcg gcataaaccg cgtttagtgg tatttcacgg gctggaaggc
     6841 agtctcaata gcccttacgc tcacggtctg gttgaggcgg cgcaaaagcg cggctggctg
     6901 ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa accgtatgca ccgcatttac
     6961 cattcgggcg aaaccgaaga cgccagttgg tttttacgct ggctgcaacg cgagtttggt
     7021 catgcgccaa cggctgccgt cggctattcg ctcggcggta atatgctggc ctgtttgctg
     7081 gcaaaagaag gtaatgatct cccgattgat gcggcggtga ttgtctctgc gccgtttatg
     7141 ctggaggcct gtagttacca tatggaaaag ggcttttccc gcatttatca gcgttacttg
     7201 ctgaacctgt taaaagccaa tgccgcgcgc aagctggcag cctaccccgg aacactgccg
     7261 attaatctcg cgcagttaaa atcggtacgt cgcatccgtg aatttgacga tctcatcacc
     7321 gccagaattc acggctacgc tgacgctatc gactattatc gtcagtgtag cgccatgccg
     7381 atgctcaatc ggatagccaa accgacactg attattcacg ccaaagacga tccgtttatg
     7441 gatcatcagg tgatcccgaa accggaaagt ctccccccgc aggtggagta tcagctgact
     7501 gaacatggcg gtcatgttgg ctttattggc ggtacattac ttcatccgca aatgtggctg
     7561 gagtcacgca ttcctgactg gttaacaacg tatctggagg cgaaatcatg ttgattccgt
     7621 ggcaagacct ctcccccgaa acgctggaaa atttaattga aagctttgtg ttacgtgaag
     7681 gcaccgatta tggtgaacat gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc
     7741 agctacagtg cggagaagcg gtgctggtat ggtcggagct gcacgaaacg gtcaatatca
     7801 tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg cggacgagat aataatgata
     7861 gctaagtcat ggagttacta tgtctgccaa acatccggtc attgcggtaa caggatccag
     7921 cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct
     7981 gcatgcagcc gaggtggaag gcgacagttt tcaccgttat acccgcccgg aaatggacat
     8041 ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa
     8101 cgacttcggc cttctggaac aaaccttcat tgaatacggt cagagtggca aagggaaatc
     8161 tcgcaaatat ctgcatacct acgatgaagc cgtaccgtgg aatcaggtac cggggacatt
     8221 caccccctgg caatctttac cggaacccac tgatgtactg ttttatgaag gtttacacgg
     8281 cggcgtagtc acgccacagc ataacgttgc gcagcatgtg gacttactgg tcggcgtggt
     8341 gcctatcgtt aaccttgagt ggattcaaaa actgatccgc gacaccagcg agcgcgggca
     8401 ctcacgagaa gcagtgatgg actcggtagt gcgttcaatg gaagactata tcaactacat
     8461 cacaccgcag ttttcccgca cccatcttaa cttccagcgc gttcccaccg tcgacacctc
     8521 aaacccgttc gcggcaaaag gtatcccgtc actggatgaa agctttgtgg tgatccattt
     8581 tcgtaatctg gaagggatcg atttcccctg gctgctggcg atgttgcaag gctcattcat
     8641 ttcccacatc aatacgttag tggtgcctgg cggcaaaatg ggtctggcaa tggaattaat
     8701 tatgctgccg ctggtgcaac gattgatgga aggaaagaaa atcgagtaac gctgctatta
     8761 cgccggataa aatactatcc ggcttcacaa cgggatagtt aaatcacgcg gcaaccactt
     8821 catacgagtg agtaatattc accgcttttt ccagcatcag cgccactgag caatatttct
     8881 cggcagaaag atcaaccgca cgcgcaaccg ctgcgtcttt cagatcgcga ccggtgacga
     8941 taaaatgtag attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgc tcagaggtca
     9001 attttacttc acaatcgacc acatcctgac gccctttttg caggatagaa accacatcga
     9061 tggcactgca accacccgcc gccatcaaca ccatttccat cgggcttggt gctttatcgc
     9121 ctgagttgcc gtccattaaa atctggtgac cagaggcgga ttcgcccaga aaagttaacc
     9181 cttcgaccca cttcactcgc gcttgcattt ttgctactcc actgcgtcaa ttttcctgac
     9241 agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg ctgaagcgag
     9301 acaccaggag acacaaagcg aaagctatgc taaaacagtc aggatgctac agtaatatat
     9361 tgatgtactg catgtatgca aaggacgcca cattaccgtg cagtacagtt gatagcccct
     9421 tcccaggtag cgggaagcat atttcggcaa tccagagaca gcggcgttat ctggctctgg
     9481 agaaagctta taacagagga taaccgcgca tggtgcttgg caaaccgcaa acagacccga
     9541 ctctcgaatg gttcttgtct cattgccaca ttcataagta cccatccaag agcacgctta
     9601 ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt taaaggctct gtggcagtgc
     9661 tgatcaaaga cgaagagggt aaagaaatga tcctctccta tctgaatcag ggtgatttta
     9721 ttggcgaact gggcctgttt gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa
     9781 ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg ccaattgatt caggtaaacc
     9841 cggacattct gatgcgtctg tctgcacaga tggcgcgtcg tctgcaagtc acttcagaga
     9901 aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat tgcacagact ctgctgaatc
     9961 tggcaaaaca accggatgct atgactcacc cggacggtat gcaaatcaaa attacccgtc
    10021 aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc
    10081 tggaagatca gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt
    10141 aatcccgtca gagtggcgcg ttacctggta gcgcgccatt ttgtttcccc cgatgtggcg
    10201 cagactgatt tatcaccccg atatcaacta tgcacttcga caaacgctgg tgctatgttt
    10261 gcccgtggcc gttgggttaa tgcttggcga attacgattc ggcctgctct tctccctcgt
    10321 tcctgcctgt tgcaatattg cgggccttga tacgcctcat aaacgttttt tcaaacgctt
    10381 aatcattggt gcgtcgctgt ttgccacctg tagcttgctg acacaggtgc tactggcaaa
    10441 agatgttccc ctgccctttt tgctgaccgg attaacgctg gtacttggcg tcactgctga
    10501 gctggggcca ttgcacgcaa aattgcttcc cgcatcgctg ctcgccgcca tttttaccct
    10561 cagtctggcg ggatacatgc cggtctggga accgttgctc atctatgcgc tgggcacttt
    10621 gtggtacgga ttgtttaact ggttttggtt ctggatctgg cgcgaacaac cgctacgcga
    10681 gtcactaagt ctgctgtacc gtgaactggc agattactgt gaagccaaat acagcctgct
    10741 tacccagcac accgaccctg aaaaagcgct gccgccgctg ctggtgcgcc agcaaaaagc
    10801 ggtcgatcta attacccagt gctatcagca aatgcatatg ctttccgcgc aaaataatac
    10861 cgattacaag cggatgctgc gtattttcca ggaggcgctg gatttgcagg aacatatttc
    10921 ggtcagtttg catcagccgg aagaggtgca aaagctggtc gagcgtagcc atgcggaaga
    10981 agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat
    11041 tctttaccat cgcctgccaa cgcgttttac gatggaaaag caaattggcg cactggaaaa
    11101 aatcgcccgc caacacccgg ataatccggt tgggcaattc tgctactggc atttcagccg
    11161 cattgctcgt gtgctgcgca cccaaaaacc gctctatgcc cgtgacttac tggccgataa
    11221 acagcggcga atgccattac ttccggcgct gaaaagttat ctgtcactaa agtctccagc
    11281 gctacgcaat gccggacgac tcagtgtgat gttaagcgtt gccagcctga tgggcaccgc
    11341 gctgcatctg ccgaagtcgt actggatcct gatgacagta ttgctggtga cgcaaaatgg
    11401 ctatggcgca acccgtctga ggattgtaaa tcgctccgta ggaaccgtgg tcgggttaat
    11461 cattgcaggc gtggcgctac actttaaaat tcccgaaggt tacaccctga ccttgatgct
    11521 gattaccacc ctcgccagct acctgatatt gcgcaaaaac tacggctggg cgacggtcgg
    11581 ttttactatt accgcagtgt ataccctgca actgttgtgg ctgaacggtg agcagtacat
    11641 ccttccgcgt cttatcgata ccattattgg ttgtttaatt gctttcggcg gtactgtctg
    11701 gctgtggccg cagtggcaga gcgggttatt gcgtaagaac gcccatgacg ctttagaagc
    11761 ctatcaggac gcgattcgct tgattctgag cgaggacccg caacctactc cactggcctg
    11821 gcagcgaatg cgggtaaatc aggcgcataa tactctgtat aactcattga atcaggcgat
    11881 gcaggaaccg gcgtttaaca gccattatct ggcagatatg aaactgtggg taacgcacag
    11941 ccagtttatt gttgagcata ttaatgccat gaccacgctg gcgcgggaac accgggcatt
    12001 gccaccggaa ctggcacaag agtatttaca gtcttgtgaa atcgccattc agcgttgtca
    12061 gcagcgactg gagtatgacg aaccgggtag ttctggcgat gccaatatca tggatgcgcc
    12121 ggagatgcag ccgcacgaag gcgcggcagg tacgctggag cagcatttac aacgggttat
    12181 tggccatctg aacaccatgc acaccatttc gtcgatggca tggcggcagc gaccgcatca
    12241 cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg taatacaggc taaaccatcg
    12301 gatgaggtgt tcacgccgca tccgacatct tttgcctgat gcgtcttatt tacgccccaa
    12361 ccaccttcgc caccgcgtgg gcgaaacgat gcatcccttc atcgatatcc gcatcttcca
    12421 ccaccagcga cggcgcaaaa cgcatcacat caggcccggc attcagcacc attacgcctt
    12481 cctctgcacc cgcatacagg aaatcacgcg cccgaccttt gtactgtggt ttcagctctg
    12541 cgccaatcaa cagccccata ccgcgaatat cgctaaacac atcgtactgc tgatcgatct
    12601 tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat accttccagc acttcagggg
    12661 tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg
    12721 tggaaccgtg agaaccagga tgaaacgcgg aagcaatttc cgccgtggtc agcatggcgc
    12781 taatcgggaa gccgccgcct aacgctttcg cagaggtcag aatatccggc gtcacgccgt
    12841 agtgcatgta agcaaacaaa tcgccggtcc gccccatccc gcactgtact tcatcaaaca
    12901 ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg
    12961 ctgccgtcac accgccctca ccctggatcg gctcaaccac caccgcacag gtgtgatcat
    13021 ccatcaccgc tttcactgcg tggagatcgt taaagggaac gtggatgatg tctgccggtt
    13081 ttggcccaaa gccatcggaa tattttggtt gcccgcccac cgagacggta aacagcgagc
    13141 gaccatgaaa agcgttatgg aaggcgataa ttttggtttt gaacgggcta tgacgcacac
    13201 aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa
    13261 acaccacgcg ttcggcaaac gttgcctcaa tcagtttacg accaagacgc agcgccggtt
    13321 cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt aacgcgttca
    13381 ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg aaatcgacat
    13441 actccttgcc ttgctgatcc cagattcggc tgccctgacc ttttaccgga ataaactctg
    13501 ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt
    13561 caattgccat ctcatgatca ccctgttacg cataaaccaa tgtgaaatta taatcacaaa
    13621 atatgcataa aaaatcactt aatagcaatc agaaatcagc gatgcaggaa attagccagc
    13681 agttgatgtc cttgttcgct aagaatactt tctggatgga attgcacacc ttccagatcc
    13741 cactggcgat ggcgaatccc cataatctcg cgggtttcgc tccaggccgt cacttcaaag
    13801 cactccggta atgagtcagg ttccaccacc agcgaatggt agcgtgtcac ggtgagtgga
    13861 tttgccagcc ccttaaatac gcccacacca ttatgtgtaa tcggcgaggt tttgccgtgc
    13921 atgacctttg cggcgcgcac aactttgccg ccaaatgcct gtgccattgc ctggtgaccg
    13981 aggcagacgc caagaatcgg caaacgcccg gcatagtgac gaataacgtc aagggagatc
    14041 ccggcttcat ctggcgtaca ggggccgggt gagatgacaa ttttttgtgg tttaagggcg
    14101 tcgatatccg ccaacgtcaa cgcatcgttg cgcttaacca acacatccgc ccccagttca
    14161 caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatcaataag caggatcatg
    14221 gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccgactt cgcttaccac
    14281 tttgctaaat atcatctgca gcgcggtgag atcccccatt gccccattct gatttgcctg
    14341 attccaggcc tctttttcga taccctgcca gctcagttga tatccggcat gaatcgccag
    14401 ttgctcgaag aagatccgct gtgccaaacc acttcccacc cggaagggat gcagcacgtt
    14461 gatttcacaa tagtaatgcg ccaaccgctc gacgaattta tctttctcaa ggctagtcag
    14521 atacttctct tcctccaaat cctgcatcag ggcattgccc tctttttcga gataggcaaa
    14581 gtggcagaac ggcgtatctc cctgataaat atcaacttca cgcagttgcc ctgcccagtc
    14641 gaaaatatcc tgatacagct ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag
    14701 cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag
    14761 ccgctgctgc tggcggatgt tgagcttgtt acgcatgata tcgagacccg ggtagagata
    14821 tggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagcgct tcaaggcgcg
    14881 ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca agacggcgac
    14941 tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc ttatcggtga
    15001 gtttcttcac ttgatg
//
LOCUS       DAOFSV010000109        14924 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.109, whole genome shotgun sequence.
ACCESSION   DAOFSV010000109 DAOFSV010000000
VERSION     DAOFSV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.109"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            259..1353
                     /gene="ddlA"
                     /locus_tag="QBH27_003430"
     CDS             259..1353
                     /gene="ddlA"
                     /locus_tag="QBH27_003430"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDQ7015706.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNNNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1377..1589)
                     /locus_tag="QBH27_003431"
     CDS             complement(1377..1589)
                     /locus_tag="QBH27_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDQ7015707.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1849..2157
                     /locus_tag="QBH27_003432"
     CDS             1849..2157
                     /locus_tag="QBH27_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDQ7015708.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2216..3310)
                     /gene="yaiW"
                     /locus_tag="QBH27_003433"
     CDS             complement(2216..3310)
                     /gene="yaiW"
                     /locus_tag="QBH27_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDQ7015709.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3323..4543)
                     /gene="sbmA"
                     /locus_tag="QBH27_003434"
     CDS             complement(3323..4543)
                     /gene="sbmA"
                     /locus_tag="QBH27_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDQ7015710.1"
                     /translation="MFKSFFPKPGMFFLSAFVWALIAVIFWQVGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4895..6052
                     /gene="ampH"
                     /locus_tag="QBH27_003435"
     CDS             4895..6052
                     /gene="ampH"
                     /locus_tag="QBH27_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDQ7015711.1"
                     /translation="MKRSLLFSAVLYSASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6053..6676)
                     /gene="iprA"
                     /locus_tag="QBH27_003436"
     CDS             complement(6053..6676)
                     /gene="iprA"
                     /locus_tag="QBH27_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDQ7015712.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6762..9749)
                     /locus_tag="QBH27_003437"
     CDS             complement(6762..9749)
                     /locus_tag="QBH27_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ7015713.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQLDISNSVIHGSITSELPFGYYSITPAEVNAKGEVVTPQTYSGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDKNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNNMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
     gene            10275..11249
                     /gene="hemB"
                     /locus_tag="QBH27_003438"
     CDS             10275..11249
                     /gene="hemB"
                     /locus_tag="QBH27_003438"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDQ7015714.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11289..12140)
                     /gene="tauD"
                     /locus_tag="QBH27_003439"
     CDS             complement(11289..12140)
                     /gene="tauD"
                     /locus_tag="QBH27_003439"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDQ7015715.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(12137..12964)
                     /gene="tauC"
                     /locus_tag="QBH27_003440"
     CDS             complement(12137..12964)
                     /gene="tauC"
                     /locus_tag="QBH27_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDQ7015716.1"
                     /translation="MSVLINEILHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSVGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(12961..13728)
                     /gene="tauB"
                     /locus_tag="QBH27_003441"
     CDS             complement(12961..13728)
                     /gene="tauB"
                     /locus_tag="QBH27_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDQ7015717.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13741..14703)
                     /gene="tauA"
                     /locus_tag="QBH27_003442"
     CDS             complement(13741..14703)
                     /gene="tauA"
                     /locus_tag="QBH27_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7015718.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3784 a         3875 c         3556 g         3709 t
ORIGIN      
        1 ttatgaaata tattctcttc gttatcttat aattaagaga aaaaaactca ccacatggtc
       61 atttaagcca ggaataaatt agcttcccgt tcatcatttt tgtgatcttg cacacatctt
      121 tgagataacg cactttcttc accagtacgt tgctcgcttt gtcagcagaa gattgcaaca
      181 ttctgtcaga aggccgcgaa tcccccgttt tgtcgtggta atgtatgcac tttgtgttag
      241 acgggatagg tttttaagat ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca
      301 gcggaacatg aagtgtctct gcaatcggca aaaaacattg tcgatgccat tgataaaagt
      361 cgcttcgatg ttgtgctgct gggcattgat aaacaagggc aatggcacgt cagcgatgcc
      421 agcaattatc tgctaaatgc agacgatcct gcccatattg cgttgcgtcc ttcggcgacc
      481 agccttgcgc aggtgccagg taaacatgag catcagctta tcgacgcaca aaacggtcag
      541 ccgttgccga cggtggatgt cattttcccg attgtccacg gtacgctggg cgaagatggt
      601 tccttgcagg gaatgctgcg ggtcgccaat ttaccgtttg taggttctga tgttctggct
      661 tcagcggcct gtatggataa agatgtcacc aaacgtctgc tgcgcgatgc cgggctgaac
      721 attgcgccat ttattaccct gacgcgcgct aatcgtcaca acatcagttt tgccgaagtg
      781 gagtctaaac tggggttacc gctgtttgta aaaccggcta atcagggctc ttctgttggt
      841 gtcagcaaag taaccagtga agaacagtac acaattgccg tcgatctggc gttcgagttc
      901 gatcataaag tgatcgttga gcaagggatc aaaggtcgtg aaatcgaatg cgcagttctt
      961 ggcaacaata acccgcaagc aagcacctgt ggcgagatcg tactcaccag cgatttctat
     1021 gcctacgaca ccaagtacat tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt
     1081 gcgccagaaa tcaacgataa gatccgggcg attgccgttc aggcttatca gacgttggga
     1141 tgcgcaggca tggcgcgtgt agacgtgttt ttaaccccag agaacgaagt ggtgatcaac
     1201 gagatcaaca ccctgcctgg cttcaccaac atcagtatgt atccgaagct gtggcaagcc
     1261 agcggtctgg gttacaccga cctgatcacg cgtttgattg agctggcgct ggagcgtcac
     1321 gctgcggata acgcactgaa aaccacaatg taatattcaa acgcccggta atattcttat
     1381 tgggcgtttt cagtttcttc atcgcgacga cgaataatca atcccgccag ccagaaacta
     1441 atcacccacg tcaccagacc gacggcatag gtctgccaac cttttgcttc aaatcccagt
     1501 aaccccacca cgccattaag aatgaatata aggccaatgg cgaacgcata gtagtgccag
     1561 tcacggcgga tttttacagg cagattcatg gcagctccgt taacgaaaaa caacatcttt
     1621 tcatattctg taacaactgt ctgtggtgta gacaacactt tcgcaattgt taataaattt
     1681 aacttaaggt tgctattttg tgactcgcaa cgaagattca cgcttcggga atattcccaa
     1741 catcaattta ccatgattct gatattgaca atctaaatga cctgtcagac ttgccataag
     1801 ccgctggtat cacaccagaa gagaagcaga tgacttccgg aggtttttat ggctgatttc
     1861 accctgtcaa aatcgctgtt tagcggaaaa tatcgcaatg cctcttcaac gcctggcaat
     1921 attgcctatg cgttgtttgt gctgttttgc ttttgggctg gggcacaatt gctgaacctg
     1981 ctagtgcatg cgcccggcgt ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca
     2041 cgggtggaaa ttggtttagg tgtcggcacc atttttgggc tgatcccgtt tttagtaggc
     2101 tgcctcattt ttgcagtagt ggcgctatgg ctgcactggc gacatcgccg ccagtaagat
     2161 ttacgttgcc agatgcggcc taaaacgcct tatctggcgt gacattcctg tttgatcact
     2221 gtttcataca acgcgcccgc cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcagat
     2281 tgcgggtgat tttcgggctt tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt
     2341 taccggtttt cgcttcggca agttgataaa ctttcttgta cagcgccgtc tcctcaaaag
     2401 agaagctgtc ccctttctct aactgacggc gaatttcgct gtcactcatc cccagttttc
     2461 ccgccagttt gcgcgttgcc agttcggttt tcccgggctc tttactgtcg taacgaatta
     2521 aatcaccgtc cagtgccagc ttcacgccgc tggccttact gaccgcgttc tgaaacgcgg
     2581 cattacggct ggcgtaccag ccagcgttaa aatcagcaaa acggtatatc ggcgcgctat
     2641 agctggcggg ataattcagt aaatggtaag taccaaacca caacccgccg cggcgactga
     2701 agacttcctg acggactgta ccgtccattt tccacggata ccctttggta tgctgttcgg
     2761 caaaagcaat actgacctgc atcggcccac cggtgcgcac cggattgagc gaaccaaaca
     2821 gcgtctgccc cattggcacc atgctgatca gatcgtcaaa aatcgcgctc aattgctttt
     2881 ctgtacgcac cgaatctaac cgttcgctat agcttttgcc gtttggcgat ttgattttta
     2941 gcgcggtatg caccagaaaa gcaggaatgt gcatccgttc agcacgacgg tcaatctctt
     3001 gccaggcgat cttgcttaag cccggaacgg ccggatctgc ctgataattt gactcctgct
     3061 gcgccaccgc cagcaccgag cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa
     3121 aggtggtggc gagatctttc gcccaggcat cgcggtcttt cacgctcgca ggcatcttct
     3181 gccgcacgac gctcgccaca tctatcgctt tttcgccctt tttcaacggt tgcggagcct
     3241 gactactaca ccccgctaaa accagaaccg ccaacaatga cagcgaagat agaggattga
     3301 cgcgcgacat tacttctcct ttttagctca aggtatgggt tacttcctga attttgtcac
     3361 catccagctc atgttcaaag ctgcgcagac gtttgtagat agacatcaac tcaaccagtg
     3421 tggtccatga gttaatcagg tactggaaag caccgcgaac ctgaccaaaa acgttagtaa
     3481 tctgcgtcat caggccgagt gtaatcgtac cggcaacaat tgacggaaac agcaagaaca
     3541 aaccgaaaac gttatcgacc tgcaaataga gaatgcgggc gatattgaaa tacatatagt
     3601 gaaaatagag gcggaaatag tttttccgta cggcgctaaa aagctcgcgt accgtaggcg
     3661 gcgtcgcgcg cgtggcatcg tcttcaccat aaaccagttc tttacggtag gcagcctcta
     3721 cacgctggtt tttaaactcc agccccggca gtttgatccc taccactgcc agtaatccgg
     3781 tccccatcag cgaccagacg atagcggcaa tcaccagacc atacggaatg tgcccgacaa
     3841 tcggcagctc cggcacatgt gcggagagcg ttaccagcac cggcaggaag gcgatcaacg
     3901 tcatgatagc gttgataaag ctgacgccca tattctccag cgttgaagca aaacgcatgg
     3961 tgtcttcctg cacacgctgc gcggcccctt cgatatgacg cagttgttgc cagttcgcca
     4021 tgtaatattc gttcatcgcc gtacgccagc ggaacacgta gtgactgaca aagaagttgt
     4081 tcagcacact gatcaccacc gcgatcagcg caatccccag aaagacgccc acttcgcggt
     4141 aaaattgttc gatggtgact ttatgcggcg aacttagcgc cgtttgaatc agatcataga
     4201 acggcgcata ccaggcgttg acggcaaccc cgacttccac caaaaaccag gtgacgaaga
     4261 taatcagcgc agtaccgagt attgaccagt attgccaacg gtgcgggcta tagataaacc
     4321 agaacaatgc aaaaagtcct acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca
     4381 acgaccagaa acgcgcggcg ctaatcggga tctgcccgga agcgccggtg atacgcgcca
     4441 cccaatcacc cccaccgact tgccagaaga taacggcaat caatgcccaa acaaaggccg
     4501 agagaaaaaa cattcccggc tttgggaaaa aagacttaaa catatgcact cctgctaact
     4561 tcttatcgtt ttaacaactg ctgtgtatta accgcatgac cgctacaaag cgtcagccaa
     4621 atgaaaccgc ccattgtgac ccatagcaag gcgcttagtt cgccagcctg accataagaa
     4681 ttgtttcgac gggtttcaca aaccaaagat tgtttacata agcggattaa cacgcgcagg
     4741 atcacaaatg caggcattgg tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt
     4801 taacactatt attgcactaa ttcgcccttt gcaatccatc aatgagtagt ataaatacgc
     4861 tcagttacct tcattcaatc tatggacacc accgttgaaa cgtagtctgc ttttttctgc
     4921 cgtgctgtat tcggcgtcat tgacctccgt ccatgcggct cagccgatca ctgaaccgga
     4981 gtttgcctct gatattgtcg atcgttatgc cgatcatatt ttttacggca gcggtgccac
     5041 ggggatggcg ctggttgtta tcgacggtaa ccagcgcgtc tttcgcagtt atggcgaaac
     5101 gcgacctggt aataacgttc gcccgcagct ggattccgtc atacgtattg cttccctcac
     5161 caagttaatg accagtgaga tgctggtaaa attgctcgac caggggaccg tgaagttaaa
     5221 cgatccgcta agtaaatatg ctccgccagg cgcacgcgta ccgacctaca atgggacgcc
     5281 cattacgctg gtgaatctgg caacccatac cagcgccctg ccccgtgaac agcccggtgg
     5341 cgcggcacat cgtccggtat ttgtctggcc aacgcgcgag caacgctgga aatacctttc
     5401 cactgccaaa ctaaaagccg cgccgggcag ccaggccgcg tactccaacc tcgcattcga
     5461 tctgctggca gatgcactgg cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga
     5521 gcagattacc cgtccgttgg ggatgaaaga caccacctac accccctcac cggatcagtg
     5581 ccgccgttta atggttgctg agcgcggtgc cagtccgtgc aataacacgc tggcggcaat
     5641 tggcagcggc ggcgtctatt ccacgccagg cgatatgatg cgctggatgc agcagtattt
     5701 gtcatctgat ttttatcaac gcagtaacca ggctgatcgc atgcagacgt tgatttatca
     5761 gcgcgcgcag tttacaaaag tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg
     5821 tttaggctgg gtatatatgg ccccgaaaga gggtcgtccg gggattattc agaagacagg
     5881 tggtggtggc ggtttcatta cctatatggc gatgatcccg cagaagaata ttggtgcgtt
     5941 tgtggtggtc actcgttcgc cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga
     6001 cctggtgacc gagctaagcg ggaataaacc gttggttatc cccgcatcct gattaatact
     6061 ctgaaggcaa acggttgata gcgaccagtt tgcctttatt catttcgata tagccgcctt
     6121 tacgcaaagc agcgagaact tccgcaacga cagaacgcga tatacgtgta cgttgatgga
     6181 tataattcat cacgccaata cgtgatcgca attcttcatt ccagtcaatc attgataata
     6241 acgtcgcgcg gatttgttcg taggaattat gcccaatgag ctgaacgtcg cgtaattcca
     6301 gaattctatt ttgccaggct aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa
     6361 taagagtaat ggtttgtttg gctggcaaat gatatcccgt acagtttcct tctgatatta
     6421 atttgtatgg tatatcgttt ttcattaaac catcagccag ccccataata taaggagcct
     6481 gtgtaatacc gataagtacg ttttcttctc tacgcagaga gataacacct tctaaaataa
     6541 cgaaagtatc ttcgctattg acatcactgg aaaatataac ttgcttttca ttattaaact
     6601 cgaagcgcgt accgtatctg gacaaacatt tatcgagctt accaaattcc tgaagaggtt
     6661 taactacaga taacatttgc gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca
     6721 ttatttagat taaattacct atttcttcgg agtgaagaat attaccaggt atatttaaca
     6781 cccacattcg cggaccagtc ttgatctacg tcaccaccac cgaggtagtt agcatcggta
     6841 taggcgctga agttcttggt gaagctgaac tgagtaccca gaccaacacg taccgcagac
     6901 ccttcagtac cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg
     6961 tagacgtaag ccagtttgaa gtacggagtc agagcctggt cttcgctgta ggtgaaggta
     7021 taacctgcat ctacacccag ttcataacgc atgctgtcgt aagactgacc atcaactttc
     7081 atgttgttgc tcagctggta atcatcacca gactggaaca gaccagaaac gctgccgtaa
     7141 ggagttacgt aacctgcatc acccagtttg aagtcgtaac cggctttcaa accaaagccc
     7201 caagcgtcgg agttggtgct gccgtcaacg taagtaccgt tgctcatggt tgcagacagg
     7261 tcgttgttga agtgagagta gctcaagcta ccgtcaacaa agacgttgtt cgcgaagtga
     7321 gcagaagagt agatgtaggc agtctggctg tcttgatcca cctgaccaga acggtcattc
     7381 atatcacctt tagcgaagcc tgcagccgca ccgacgatcc acttagcgtt gttaccgtca
     7441 attttggtat caacaccgac catgatgccg ttaacatcct gatcatagtt gatggtgccg
     7501 ttgtcgccgt tgaagttacc accgaagtag cttacccatg cgccgccgtt atcagccaga
     7561 ccatgacgag agttggtcag acgagtacca acggtgtctt gttccaggtt ccagatattg
     7621 gtgttcgcag acgggatgct cagcgccatg ttagcgtagt cggtcagctc catctgttgc
     7681 agaacaacgg tgttaccacg ctgctcagcc tgataggtgt atgcacccag gtcagctttg
     7741 ttagcagcag agaaagtcgc gttgctgttt ttgtcgttga cgtagataat ttctttatct
     7801 ttgtaatcag cgattgcgcc agcgccagtt gcgttgtcga tacgaacttt gtagttaccg
     7861 gttgcagcaa caacgttgtc tacagaactg ttatccagtt cagtaccgtt gtctacgtcg
     7921 ccgttgccat tgatagtcag gtgaccatca gagttcatag caacaatacc gtaaccgtag
     7981 ttagacttgg aagtatccca cgtacggtcg ttcaccaaat ccgcgttcag aacgtaatcg
     8041 ctgctgttga tgtcgaacac accagaaact acgttgccgt tatggtcagc gatgttgccg
     8101 ctagtcagtt ccagtttgtc agtacggaaa gcttcagtat caaactgatc aacgttcaca
     8161 tccagtacac caccgttagt tacggtgatg gtattggcat acaggttaga atagttattc
     8221 cagccagtag attcggttaa attaacggta ctgtagctat caacagtcag gtggtctgta
     8281 gcaaccaaac catcttcacc aatattcagt gaggagccgt tggttaaacc aatggtatct
     8341 gagaccagag aggaatcagt aacatttacc tgagagccat tatttacagc taatgtatca
     8401 attactgaac tattaacggt attccattct gaaccattgt tcagtgctac gttaaatacg
     8461 ccgctctgga ataccttgtt gtcaataata cgaccccaat cgttagcttc gatagtggca
     8521 ccaggggtca ggctgttcgg cgcaacatca tacatatctg ccggagcaat tacttcagca
     8581 ctcatgtttg ctgagccaac ccacttacta ccgttgttca gagtaatgtt cagttcatca
     8641 acgttctgag catcatctac ccaaccaccg ttggtatcaa ggacaccgtc accattgctg
     8701 tcatggccgg ttgaatagaa gaatacgcca tcgttattcc agtggcttgt gaactcgaca
     8761 tcacccaaca gggttgaatg gtcaaagttt acattgtttt tcatggaata atttgagcca
     8821 gcattgtcag agaatgacag agctacgtca ttttcgccac cattaccagc gtaatcactt
     8881 ggttcagcag atttaccgaa gaagccattg ctttcttcca gcggagtctg tgaccctgaa
     8941 gttacagtgg agttagacac attaatgttg ttgtcccact gatgatattc gctagtataa
     9001 gtcgtgttgc ttgtattgcc ttcagacagc gcaataccag ctacacggga gttgttagta
     9061 atattgatat tgctttcaac atcaagattt actgcaacac ccagagcggt atagaacgtt
     9121 tcattggttg acttagattt atcaccatca agatatgtgt cagtgaagta aagagcttca
     9181 tagtcatcat caatggtgga attggatacg ttcagagtga atacgtcacc atcgtaccaa
     9241 ttgtagtcaa ggtcagtagt gattccgtta tagaaattat agccactata ggtctgtgga
     9301 gttacaactt cacctttagc attaacttct gcaggagtaa tgctgtaata accaaatggc
     9361 agttcagaag tgattgaccc atgaattact gaattgctga tatctaactg gttaacagaa
     9421 ccgctagcgt aatcatgatt caagtaataa gtagaaatgg taccgttaac agtatcgcct
     9481 ttaaagacgg tgtcgaaatc accattggca ttgttatagt aaacaaaacc attgtaagtt
     9541 acgtagttac cgtctgaacc catcgggtcc atgccgccag catcggcaat tccatcattg
     9601 tcagtgtcat acggccaaac taaatcggtc gtggtgttgt catgataccc ccaggtatca
     9661 tagtttgctg cattagcctg agaggtgata gccagagtgc aagccaatgc taattgtgat
     9721 actacaagtt tctttttcca ggagtgcatt tgtcatccct cctcagggac gtaataagtt
     9781 gatccatcaa ttatcaatgc atagaaaaca tattatgttt cgcgtttacg attatgcggc
     9841 gttatagagg aatggttcaa ttattctttc ccctaagtcc gattgcggac atatataaag
     9901 cacgtgaaca cacacacaga aataaataac aaaaacacaa tatttcaata cgttaatgaa
     9961 agcgttaatt ttaaaacata gaccataaaa atataaaaca gctaaaacag acaaataatg
    10021 tatcaataag caatgaactt taagttattc actttttgaa aacgtcacca aatccccaaa
    10081 aacaataatc tatggctata aataaaaatt aaaaatattt tcagtgaata tgaaattatt
    10141 gctatttcta ttacaaagat gtcctgacct ctttttcatt ctcttcttgt caacatcgcg
    10201 acaactttcg taaaacacac ccagcctgct tcaggtatac tatgcccctc gattccacaa
    10261 acatcaggca gaccatgaca gacttaatcc aacgccctcg tcgcctgcgc aaatctcctg
    10321 cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa cgacctggtg ttgccgatct
    10381 ttgttgaaga agaaattgac gactataaag ccgttgaagc catgccaggc gtgatgcgca
    10441 ttccagagaa acatctggca cgcgaaattg aacgcatcgc caacgccggt attcgttccg
    10501 tgatgacttt cggcatctct caccataccg atgaaaccgg cagcgatgcc tggcgggaag
    10561 atggactggt ggcgcgtatg tcgcgcatct gcaagcagac cgtgccagaa atgatcgtta
    10621 tgtcagacac ctgcttctgt gaatacacat ctcacggtca ctgcggtgtt ctgtgcgagc
    10681 atggcgtcga caacgacgcg actctggaaa atttaggcaa gcaagccgtg gttgcagctg
    10741 ctgcaggcgc agacttcatc gccccttctg ccgcgatgga tggtcaggta caggcgattc
    10801 gccaggcgct ggacgctgcg ggctttaaag atacggcgat tatgtcgtat tcgaccaagt
    10861 tcgcctcctc cttttatggc ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc
    10921 gcaaaagcta tcagatgaac ccaatgaacc gtcgtgaggc gattcgtgag tcactgctgg
    10981 atgaagccca gggcgcagac tgcctgatgg ttaaacctgc cggagcatac ctcgacatcg
    11041 tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc gtatcaggtg agcggtgagt
    11101 acgcgatgat taagttcgcc gcgctggcgg gtgctataga tgaagagaaa gtcgtgctcg
    11161 aaagcttagg ttcgattaag cgtgcgggtg cggatctgat tttcagctac tttgcgctgg
    11221 atttggctga gaagaagatt ctgcgttaat gatcattatg ccgggttatc caacccggca
    11281 tttccccctt accccgcccg ataaaacgga atatccccaa gaatcgtcgc ccgatgcata
    11341 atccgccgct gtggcaggta atccgcattg gcatagtgct gagtcactcg gttatcccaa
    11401 atcgcaatat cattcggttg ccagcgccag cgcacctgaa actccggttt agtgatatgg
    11461 gcaaacaaaa aacctaacaa ggcttcgctc tctttctcgc tgacatcaac aattcgtgtg
    11521 gtaaaacctt cgttcacaaa cagtgcctgt ttaccggtca ccggatgcgt tcgcaccacc
    11581 ggatgtagca acggtggatt tttcgcgacc gcctcacgcc agcgttgatg ctcagcctcg
    11641 gttttgcggt acttgtattc cgggaacgat ttacggaaat catgctccgc acgcaacccg
    11701 ctcaacagct ggcggaaggg aacagagagc gcctcatatg cggcaatacc actggcccag
    11761 agcgtatcgc cgccggtcga gggtaactct ttcgccgcca gaatcgcccc tgcgggtggc
    11821 gtttgaataa atgtcacatc ggtgtgccag ttgtcgttat ccggcggatt atcattatgg
    11881 gtatccagga cgatgatctc gtcaacccct tcagcatgag ggtaaacagg gtgaatatgc
    11941 agctcgccaa aacgctgggc cagcgcgcgt tgctgctgcg gcgtgatagc ttgatcgcgc
    12001 agaaacacca cctgatggcg cagcaccgca tggtaaagct gttcaaactg attatcgctt
    12061 aacgggcgcg ttaagtcggc ccccgtaatt tgtgcgccaa tatacggccc cagcggggta
    12121 atgctcagac gttcactcat tgtacttctc catgccaggg cgtcaggcgg cgctgtaacg
    12181 cgcgcagacc cagttctaaa agaaaggcga taatcgcaat caccgcgatc cccgccagca
    12241 ccacgtcagt cgcgagaaat tcacccactg actgaaccat aaatcctaaa ccgcgcgttg
    12301 cggcaatcag ctccgccgcc accagcgtag accagcccac acccagacca atgcgtaatc
    12361 cggtgaggat ttccggcagc gcgccgggca aaatgacaaa ccacagcacc tgcgcacggc
    12421 tggcacccag cgactgggcg gcacgaatgc gaacctgctg cgcgcttttc acccccgcca
    12481 gcgccgacat cgccaccggc gcaaaaatcg ctaaatagat cagcaagatc ttcgaggttt
    12541 cgccaatacc aaaccagatc accatcagcg gcaaataagc cagcggcggc accggacgat
    12601 aaagctcgat tatcggatcc agaatgccgc gcaccgtcgg gcttagcccc atggcgatcc
    12661 cgaccggaat accgatcaac accgccgcga gtagcgccag caaaatgcgc gtcaggctgg
    12721 ctgccagatg ctgccacagc gtggcgtcca taaatccctg cggcccggca atggtgagca
    12781 gttttgccag tacctgttgc ggtggcggca aaaacagcgg gctaatcagt tgcagcgccg
    12841 cgactgccca ccatacagtg agcaaaaccg ctaacgtacc aatgcttaag gtcacctgac
    12901 gcgagagcgg ccagcgccat ttcagccgat gcgaatgcag tatttcatta atgagcacac
    12961 tcatgagaac gcctcccgtt gctcaaatac gcggcttaaa acatattcgc gcatggcgat
    13021 gaattgtgga tcggacttga tgctgcggct cgactctccc gcaacaaagc ggcgagcaaa
    13081 gttgagcggc agccgctcca gcacacggcc tgggccgggt gaaagcaaaa ccagttcagt
    13141 cgccataaac accgcttctt ctatatcgtg ggtaatcaac agcacctgct ttcccgtctc
    13201 ctgccaaagt ttcagcagca gggtttgcat ctggtcgcgg gtgaaggcgt ccagcgcacc
    13261 aaacggttcg tcgagtaata acagctgggg atttgccgcc aacgcgcggg caatccccac
    13321 ccgctgacgt tgaccaccgg aaagctgcca gatgtagcgt ttttctgcgc cttccagccc
    13381 gacttttttc accatctggt gtgcgatttc cagtcgctgc attttctcta cacctgccag
    13441 ttgcaggccg aacgccacgt tgtcctgtac attgcgccac ggtaacaacc cttcattctg
    13501 aaaaactacg ccacgctctg ctcccggtcc ctgaataccc ttacccgcca gttgaatgct
    13561 gccatgctga taaggcacaa aaccagcaat caaattcagc aacgtagttt tgccgcagcc
    13621 agacggcccc agaactacca gaagctcgcc gctttccagc gtcaggttga tatcttccag
    13681 tgccggtttg ccgccataat cggcgtaaag atgagaaatt tgcagcatct gcgcctcctt
    13741 ttattgcacg aagcgcgagg taacgtactg gctgtaatca ttcgctacag ccgggacttt
    13801 gccctgctct ttcaaaaact gcgcggtgtc gatgatcgct ttgttcaccg gtccggtcag
    13861 ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc ttcaccagcc ccggaacgtc
    13921 accttccggc acgccgctta accgcgccag tttgctgatg ttttccggct gtttcagcca
    13981 cgcgtctggg ttagcaatgt acggttgctg agcatcgatg gcgcttttag cgaacgcttt
    14041 cacgacctca ggatgtttct cggcaaaatc tttccgcacg acccagacgt ccagtgtcgg
    14101 cgcgccccac tgcccgacct gttcagaatc ggtcagcacc ttgccatctt tttccagggc
    14161 gttaaccgcc ggtgcccata cataagcacc atcaatatct ccccgctgcc atgcggcgat
    14221 aattgcgggc ggctgcaggt tcacaatctc aacttgccca ggtttaatac cccagtgttt
    14281 cagcgccgcc agcaggctgt agtgggtggt ggagataaac ggtacggcga tgcgtttacc
    14341 aatcaagtct tccggtttac tgatagtttt ctttaccacc agcgcttcgg agttacccag
    14401 ttttgacgcc agcaagaaga cttcaatcgg cacctgttgg ctggttgcaa ccgctaacgg
    14461 gctggaaccg aggttgccga tttgcacatc gcccgaagcc agcgcccgca cgatgctggc
    14521 tccactgtca aacttacgcc agtccacggt tgctccgctt tctttagcaa aggtgttgtc
    14581 ggcctgagcc actttcgccg gttcggcgga ggtttgatac gccacggtga cgttcaccgc
    14641 ttgcgcctga aaagcgatga atgccagtgc ggcaagaaat gtgttacgcg atgaaattgc
    14701 catgattgtc tgctcccccg tcttgttatg ggagcagtat ttcgttgcgg gtaagtttga
    14761 taaaggaatt aaaacgtctt gttaataaca aagcgttttt agaaaaaaat cggcaatggt
    14821 tagcgagttt gcgcattttg ctacttttaa caaacatcgc ctgatgtgac gctcacgcct
    14881 cttatcatgc ctacaaatac tggggccgga taaattttat tctc
//
LOCUS       DAOFSV010000110        14892 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.110, whole genome shotgun sequence.
ACCESSION   DAOFSV010000110 DAOFSV010000000
VERSION     DAOFSV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.110"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..2440
                     /gene="thrA"
                     /locus_tag="QBH27_003443"
     CDS             <1..2440
                     /gene="thrA"
                     /locus_tag="QBH27_003443"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDQ7015719.1"
                     /translation="TSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIE
                     KTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISL
                     LGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIA
                     ESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIW
                     TDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKN
                     TGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRA
                     RISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIIS
                     VVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQM
                     LFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGL
                     NLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYADFLREGFHV
                     VTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKF
                     SGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARET
                     GRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEGKVLRYVGN
                     IDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAGNDVTAAGV
                     FADLLRTLSWKLGV"
     gene            2442..3374
                     /gene="thrB"
                     /locus_tag="QBH27_003444"
     CDS             2442..3374
                     /gene="thrB"
                     /locus_tag="QBH27_003444"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDQ7015720.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            3375..4661
                     /gene="thrC"
                     /locus_tag="QBH27_003445"
     CDS             3375..4661
                     /gene="thrC"
                     /locus_tag="QBH27_003445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDQ7015721.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHGGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            4875..5171
                     /locus_tag="QBH27_003446"
     CDS             4875..5171
                     /locus_tag="QBH27_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDQ7015722.1"
                     /translation="MKKMQSIVLALSLVLFAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(5211..5987)
                     /gene="yaaA"
                     /locus_tag="QBH27_003447"
     CDS             complement(5211..5987)
                     /gene="yaaA"
                     /locus_tag="QBH27_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDQ7015723.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6057..7487)
                     /locus_tag="QBH27_003448"
     CDS             complement(6057..7487)
                     /locus_tag="QBH27_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDQ7015724.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            7766..8719
                     /gene="tal"
                     /locus_tag="QBH27_003449"
     CDS             7766..8719
                     /gene="tal"
                     /locus_tag="QBH27_003449"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDQ7015725.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8834..9421
                     /gene="mog"
                     /locus_tag="QBH27_003450"
     CDS             8834..9421
                     /gene="mog"
                     /locus_tag="QBH27_003450"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDQ7015726.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(9456..10022)
                     /gene="satP"
                     /locus_tag="QBH27_003451"
     CDS             complement(9456..10022)
                     /gene="satP"
                     /locus_tag="QBH27_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDQ7015727.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10171..10884)
                     /gene="msyB"
                     /locus_tag="QBH27_003452"
     CDS             complement(10171..10884)
                     /gene="msyB"
                     /locus_tag="QBH27_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDQ7015728.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(10910..11314)
                     /locus_tag="QBH27_003453"
     CDS             complement(10910..11314)
                     /locus_tag="QBH27_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDQ7015729.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11685..13601
                     /gene="dnaK"
                     /locus_tag="QBH27_003454"
     CDS             11685..13601
                     /gene="dnaK"
                     /locus_tag="QBH27_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDQ7015730.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            13690..14820
                     /gene="dnaJ"
                     /locus_tag="QBH27_003455"
     CDS             13690..14820
                     /gene="dnaJ"
                     /locus_tag="QBH27_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDQ7015731.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         3752 a         3690 c         4007 g         3443 t
ORIGIN      
        1 tacatcagtg gcaaatgcag aacgttttct gcgggttgcc gatattctgg aaagcaatgc
       61 caggcagggg caggtggcga ccgtcctctc tgcccccgcc aaaattacca accatctggt
      121 agcgatgatt gaaaaaacta ttagcggcca ggatgcttta cccaatatca gcgatgccga
      181 acgtattttt gccgaacttc tgacgggact cgccgccgcc cagccgggat ttccgctggc
      241 acaattgaaa actttcgtcg accaggaatt tgcccaaata aaacatgtcc tgcatggcat
      301 cagtttgttg gggcagtgcc cggatagcat caacgctgcg ctgatttgcc gtggcgagaa
      361 aatgtcgatc gccattatgg ccggcgtgtt agaagcgcgt ggtcacaacg ttaccgttat
      421 cgatccggtc gaaaaactgc tggcagtggg tcattacctc gaatctaccg ttgatattgc
      481 tgaatccacc cgccgtattg cggcaagccg cattccggct gaccacatgg tgctgatggc
      541 tggtttcact gccggtaatg aaaaaggcga gctggtggtt ctgggacgca acggttccga
      601 ctactccgct gcggtgctgg cggcctgttt acgcgccgat tgttgcgaga tctggacgga
      661 tgttgacggt gtttatacct gcgatccgcg tcaggtgccc gatgcgaggt tgttgaagtc
      721 gatgtcctat caggaagcga tggagctttc ttacttcggc gctaaagttc ttcacccccg
      781 caccattacc cccatcgccc agttccagat cccttgcctg attaaaaata ccggaaatcc
      841 tcaagcacca ggtacgctca ttggtgccag ccgtgatgaa gacgaattac cggtcaaggg
      901 catttccaat ctgaataaca tggcaatgtt cagcgtttcc ggcccgggga tgaaagggat
      961 ggttggcatg gcggcgcgcg tctttgcagc gatgtcacgc gcccgtattt ccgtggtgct
     1021 gattacgcaa tcatcttccg aatacagtat cagtttctgc gttccgcaaa gcgactgtgt
     1081 gcgagctgaa cgggcaatgc aggaagagtt ctacctggaa ctgaaagaag gcttactgga
     1141 gccgttggcg gtgacggaac ggctggccat tatctcggtg gtaggtgatg gtatgcgcac
     1201 cttacgtggg atctcggcga aattctttgc cgcgctggcc cgcgccaaca tcaatattgt
     1261 cgccattgct cagggatctt ctgaacgctc aatctctgtc gtggtcaata acgatgatgc
     1321 gaccactggc gtgcgcgtta ctcatcagat gctgttcaat accgatcagg ttatcgaagt
     1381 gtttgtgatt ggcgtcggtg gcgttggcgg tgcgctgctg gagcaactga agcgtcagca
     1441 aagctggttg aagaataaac atatcgactt acgtgtctgc ggtgttgcta actcgaaggc
     1501 actgctcacc aatgtacatg gccttaatct ggaaaactgg caggaagaac tggcgcaagc
     1561 caaagagccg tttaatctcg ggcgcttaat tcgcctcgtg aaagaatatc atctgctgaa
     1621 cccggtcatt gttgactgta cttccagcca ggctgtggca gatcaatatg ccgacttcct
     1681 gcgcgaaggt ttccacgttg ttacgccgaa caaaaaggcc aacacctcgt cgatggatta
     1741 ctaccatcag ttgcgttatg cggcggaaaa atcgcggcgt aaattcctct atgacaccaa
     1801 cgttggggct ggattaccgg ttattgagaa cctgcaaaat ctgctcaatg ctggtgatga
     1861 attgatgaag ttctccggca ttctttcagg ttcgctttct tatatcttcg gcaagttaga
     1921 cgaaggcatg agtttctccg aggcgaccac actggcgcgg gaaatgggtt ataccgaacc
     1981 ggacccgcga gatgatcttt ctggtatgga tgtggcgcgt aaactattga ttctcgctcg
     2041 tgaaacggga cgtgaactgg agttggcgga tattgaaatt gaacctgtgc tgcccgcaga
     2101 gtttaacgcc gagggtgatg ttgccgcttt tatggcgaat ctgtcacagc tcgacgatct
     2161 ctttgccgcg cgtgtggcga aggctcgtga tgaaggaaaa gttttgcgct atgttggcaa
     2221 tattgatgaa gatggcgtct gccgcgtgaa gattgccgaa gtggatggta atgatccgct
     2281 gttcaaagtg aaaaatggcg aaaacgccct ggccttctat agccactatt atcagccgct
     2341 gccgttggta ctgcgcggat atggtgcggg caatgacgtt acagctgccg gtgtctttgc
     2401 tgatctgcta cgtaccctct catggaagtt aggagtctga catggttaaa gtttatgccc
     2461 cggcttccag tgccaatatg agcgtcgggt ttgatgtgct cggggcggcg gtgacacctg
     2521 ttgatggtgc attgctcgga gatgtagtca cggttgaggc agcagagaca ttcagtctca
     2581 acaacctcgg acgctttgcc gataagctgc cgtcagagcc acgggaaaat atcgtttatc
     2641 agtgctggga gcgtttttgc caggagcttg gcaagcaaat tccagtggcg atgactctgg
     2701 aaaagaatat gccgatcggt tcgggcttag gctccagcgc ctgttcagtg gtcgcggcgc
     2761 tgatggcgat gaatgaacac tgcggcaagc cgcttaatga cactcgtttg ctggctttga
     2821 tgggcgagtt ggaagggcgt atctccggca gcattcatta cgacaacgtg gcaccgtgtt
     2881 ttcttggtgg tatgcagttg atgattgaag aaaacgacat catcagtcag caagtgccag
     2941 ggtttgatga gtggctgtgg gtgctggcgt atccggggat taaagtctcg acggcagaag
     3001 cccgggctat tttaccggcg cagtatcgcc gccaggattg cattgcgcac gggcgacatt
     3061 tggcaggatt cattcacgcc tgctattccc gtcagcctga gcttgccgcg aagctgatga
     3121 aagatgttat cgctgaaccc taccgtgaac ggttattgcc tggcttccgg caggcgcggc
     3181 aggcggttgc ggaaatcggc gcggttgcga gcggtatctc cggctccggc ccgaccttgt
     3241 tcgctctgtg tgacaagccg gataccgctc agcgcgttgc cgactggttg ggtaagaact
     3301 acctgcaaaa tcaggaaggt tttgttcata tttgccagct ggatacggcg ggcgcacgag
     3361 tactggaaaa ctaaatgaaa ctctacaatc tgaaagatca caatgagcag gtcagctttg
     3421 cgcaagccgt aacccagggg ttaggcaaaa atcaggggct gttttttccg cacgacctgc
     3481 cggaattcag cctgactgaa attgatgaga tgctgaagct ggattttgtc acccgcagtg
     3541 cgaagatcct ctcggcgttt attggtgatg aaatcccgca ggaaatcctg gaagagcgcg
     3601 tacgtgcggc gtttgccttc ccggctccgg tcgccaatgt tgaaagcgat gtcggttgtc
     3661 tggaattgtt ccacgggcca acgctggcat ttaaagattt cggcggtcgc tttatggcac
     3721 aaatgctgac ccatattgcg ggcgataagc cagtgaccat tctgaccgcg acatccggtg
     3781 atactggagc ggcagtggct catgctttct acggtttacc gaatgtgaaa gtggttatcc
     3841 tctatccacg aggcaaaatc agtccactgc aagaaaaact gttctgtaca ttgggcggca
     3901 atatcgaaac tgttgccatc gacggcgatt tcgatgcctg tcaggcgctg gtgaagcagg
     3961 cgtttgatga tgaagagctg aaagtggcgc tggggctaaa ctctgctaac tccattaaca
     4021 tcagccgttt gctggcgcag atttgctact actttgaagc cgtggcgcag ttgccgcagg
     4081 aagcgcgcaa ccagctggtt gtctcggtgc cgagcggtaa cttcggcgat ttgacagcgg
     4141 gcctgctggc gaaatctctt ggcctgccgg tgaagcgttt tattgctgcg accaacgtga
     4201 atgataccgt gccacgtttc ctgcacggcg gtcagtggtc acccaaagcg actcaggcga
     4261 cgttatccaa tgcgatggat gttagccagc caaacaactg gccgcgtgtg gaagagttgt
     4321 tccgccgcaa aatctggcaa ctgaaagagc tggggtatgc agccgtggat gatgaaacca
     4381 cgcaacagac aatgcgtgag ttaaaagaac tgggctatac ctcggagccg cacgctgccg
     4441 tagcttatcg tgcgctgcgt gaccagttga atccaggcga atatggcttg ttcctcggca
     4501 ccgcgcatcc ggcaaaattt aaagagagcg tggaagcgat tctcggtgaa acgttggatc
     4561 tgccaaaaga gctggcagaa cgtgctgatt tacccttgct ttcgcataac ctgcccgccg
     4621 attttgctgc gttgcgtaaa ttgatgatga atcatcagta acatctattc attatctcaa
     4681 tcaggccggg tttgctttta tgcagcccgg cttttttatg aagaaaatat ggagaaaaac
     4741 gacagggaaa aaggagaaat tctcaataaa tgcggtaact tagagattag gattgcggag
     4801 aataacaact gccgttctca tcgcgtaatc tccggatatc gacccataac gggcaatgat
     4861 aaaaggagta acctgtgaaa aagatgcaat ctatcgtact cgcactttcc ctggttctgt
     4921 tcgctcccat ggcagcgcag gctgcggaaa ttacgctagt cccgtcagta aaattacaga
     4981 taggcgatcg tgataatcgt ggctattact gggatggcgg tcactggcgc gaccacggct
     5041 ggtggaaaca acattatgaa tggcgaggca atcgctggca cccatatgga ccgccgccat
     5101 cgccgcgcca taacaagcac aatgatcatc gtggcgatca tcgtccgggg cctgacaaac
     5161 atcatcgcta aatatcaatg ccggattgcc atccggcatt gactacaaac ttaacgctgc
     5221 tcgtagcgtt taaacaccag ttcgccattg ctggaggaag cttcatcaaa gaagtaacct
     5281 tcgctattaa aaccagttag ttgctctggt ttggtcagcc gattttcaat aatgaaacga
     5341 ctcatcagac cgcgtgcttt cttagcgtag aagctgatta tcttaaattt gccgttcttc
     5401 tcatcgagga acactggctt gataatctcg gcattcaatt tcttcggctt caccgattta
     5461 aaatactcat ctgacgccag atttatcact acattatcgc cttgtgctgc gagcgcctcg
     5521 ttcagcttgt tggtgatgat atctccccag aattgataca gatctttccc tcgggcattc
     5581 tcaagacgta tccccatttc cagacgataa ggctgcatta aatcgagcgg gcgcagtacg
     5641 ccatacaagc cggaaagcat tcgcaaatgc tgttgggcaa aatcgaaatc gtcttcgctg
     5701 aaggtttcgg cctgcaagcc agtgtagaca tcacctttaa acgccagaat tgcctggcgg
     5761 gcattttccg gtgtgaaatc tggctgccag tcatgaaagc gagcggcgtt gatacccgcc
     5821 agtttatcgc tgatgcgcat cagggagcta atctgcgggg gcgtcagttt ccgcgcttca
     5881 tggatcagct gctgggagtt gtctaacagc tccggcagcg tatagcgcgt ggtggtcaac
     5941 gggctttggt aatcaagcgt tttcgcaggt gaaataagaa tcagcatatc cagtccttgc
     6001 aggaaattta tgccgacttt agcaaaaaaa gagaatgagt tgatcgatag ttgtgattac
     6061 tcctgcgaaa catcatccca cgcgtccgga gaaagctggc gaccgatctc cggataacgc
     6121 aacggatcaa acaccgggcg cacgccgagt ttacgctggc gtagataatc actggcaatg
     6181 gtatgaacca caggcgagag cagtaatatg gcggtcaaat tggtaatagc catgcaggcc
     6241 ataatgatat ctgccagttg ccacatcagc ggaagactta gcaaggtgcc gccgatgacc
     6301 gttgcgaagg tgcagatccg cagacaccag atcgctttag gggtgttcag gcgtaaaaag
     6361 aagagattgt tttcggcata aatgtagttg gcaacgatgg agctgaaggc aaacagaata
     6421 accacgaggg taacaaactc cgcaccccag gaacccatta gcacccgcat cgccttctgg
     6481 ataagctgaa taccttccag cggcatgtag gttgtgccgt tacccgccag taatatcagc
     6541 atggcgcttg ccgtacagat gaccagggtg tcgataaaaa tgccaatcat ctggacaatt
     6601 ccttgcgctg ccggatgcgg aggccaggac gcagctgccg ctgccgcgtt tggcgtcgac
     6661 cccattcccg cctcattgga aaacatactg cgctgaaaac cgttagtaat cgcctggctt
     6721 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
     6781 gaccaaatga cgtggggaag ttgcccgata ttcattacgc aaatcaccag gctggtcagt
     6841 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
     6901 ccgcgagtga tcgccagcat agcaaagaca gcgagaataa tacctgtcac cagcggggga
     6961 aaatcaaaag aaaaactcag ggctcgcgca acggcgttcg cctgaactcc gctgaaaatt
     7021 atgccgtagg cgatgagcaa aaagacggcg aacagaacgc ccatccagcg catccccagc
     7081 ccgcgcgcca tataccatgc cggtccgcca cgaaactgcc cattggcatc acgttcttta
     7141 taaagctgtg cgagggaaca ttcggcaaac gaggtcgcca tgccgataaa tgcggcaacc
     7201 cacatccaga agacggctcc aggtccaccg gcggtaattg ccagcgcaac gccggccagg
     7261 ttgccgctac ccacgcgcgc tgcaagactg gtacacaatg actgaaatga ggttaaaccg
     7321 cctggctgtg gatgaatgct atttttaaga cttttgccaa actggcggat gtagcgaaac
     7381 tgcacaaatc cggtgcgaaa agtgaaccaa caacctgcgc cgaagagcag gtaaatcatt
     7441 accgaacccc aaaggacgct gttaataaag gagaaaaaat ctggcatgca tatccctctt
     7501 attgccggtc gcgatgactt tcctgtgtaa acgttaccaa ttgtttaaga agtatatacg
     7561 ctacgaggta cttgataact tctgcgtagc atacatgagg ttttgtataa aaatggcggg
     7621 cgatatcaac gcagtgtcag aaatccgaaa cagtctcgcc tggcgataac cgtcttgtcg
     7681 gcggttgcgc tgacgttgcg tcgtgatatc atcagggcag accggttaca tccccctaac
     7741 aagctgttta aagagaaata ctatcatgac ggacaaattg acctcccttc gtcagtacac
     7801 caccgtagtg gccgacactg gggacatcgc ggcaatgaag ctgtatcaac cgcaggatgc
     7861 cacaaccaac ccttctctca ttcttaacgc agcgcagatt ccggaatacc gtaagttgat
     7921 tgatgatgct gtcgcctggg cgaaacagca gagcaacgat cgcgcgcagc agatcgtgga
     7981 cgcgaccgac aaactggccg taaatattgg tctggaaatc ctgaaactgg ttccgggccg
     8041 tatctcaact gaagttgatg cgcgtctttc ctatgacacc gaagcgtcaa ttgcgaaagc
     8101 aaaacgcctg atcaaactct acaacgatgc aggtattagc aacgatcgta ttctgatcaa
     8161 actggcttct acctggcagg gtatccgtgc tgcggaacag ctggaaaaag aaggcatcaa
     8221 ctgtaacctg accctgctgt tctccttcgc tcaggctcgt gcttgtgcgg aagcgggcgt
     8281 gttcctgatc tcgccgtttg ttggccgtat tcttgactgg tacaaagcga ataccgataa
     8341 gaaagagtac gctccggcag aagatccagg cgtggtttct gtatctgaaa tctaccagta
     8401 ctacaaagag cacggttatg aaaccgtggt tatgggcgca agcttccgta acatcggcga
     8461 aattctggaa ctggcaggct gcgaccgtct gaccatcgca ccggcactgc tgaaagagct
     8521 ggcggagagc gaaggggcta tcgaacgtaa actgtcttac actggtgaag tgaaagcgcg
     8581 tccggcgcgt atcactgagt ccgagttcct gtggcagcac aaccaggatc caatggcagt
     8641 agataaactg gcggaaggta tccgtaagtt tgctgttgac caggaaaaac tggaaaaaat
     8701 gatcggcgat ctgctgtaat cattcttagc gtgaccggga agtcggtcac gctacctctt
     8761 ctgaaacctg tctgtcactc ccttcgcagt gtatcattct gtttaacgag actgtttaaa
     8821 cggaaaaatc ttgatgaata ctttacgtat tggcttagtt tccatctctg atcgcgcatc
     8881 cagcggcgtt tatcaggata aaggcatccc tgcgctggaa gaatggctga catcggcgct
     8941 aaccacgccg tttgaactgg aaacccgctt aattcccgat gagcaggcga tcatcgagca
     9001 aacgttgtgt gagctggtgg atgaaatgag ttgccatctg gtgcttacca cgggcggaac
     9061 tggcccggcg cgtcgtgacg taacgcccga tgcgacgctg gcagtagcgg accgcgagat
     9121 gccaggcttt ggtgaacaga tgcgccagat cagcctgcat tttgtaccaa ctgcgatcct
     9181 ttcgcgtcag gtgggggtga ttcgcaaaca ggcgctgatc cttaacttac ccggtcaacc
     9241 gaagtctatt aaagagacgc tggaaggtgt gaaggacgct gagggtaacg ttgtggtgca
     9301 cggtattttt gccagcgtac cgtactgcat tcagttgctg gaagggccat acgttgaaac
     9361 ggcaccggaa gtggttgcag cattcagacc gaagagtgca agacgcgaag ttagcgaata
     9421 aaaaaatccc cccgagcggg gggatctcaa aacaattagt gggattcacc aatcggcaga
     9481 acggtgcgac caaactgctc gttcagcact tcacccatcg ccagatagat tgcgctggca
     9541 ccgcagataa gaccaatcca gccggcaaag tggatgatcg cggcgttacc ggcaatgtta
     9601 ccgatcgcca gcagggcaaa cagcacggtc aggctaaaga aaacgaattg cagaacgcgt
     9661 gcgcctttca gcgtgccgaa gaacataaac agcgtaaata tgccccacag acccaggtag
     9721 acaccaagga actgtgcatt tggcgcatcg gtcagcccca gtttcggcat cagcagaatt
     9781 gcaaccagcg tcagccagaa agaaccgtaa gaggtgaatg cggttaaacc gaaagtgttg
     9841 ccttttttgt actccagcag accggcaaaa atttgcgcga tgccgccgta gaaaatgccc
     9901 atggcaagaa taataccgtc cagagcaaaa taacccacgt tgtgcaggtt aagcagaatg
     9961 gtggtcatgc cgaagcccat caggcccagc ggtgccggat tagccaactt agtgttgccc
    10021 ataattcctc aaaaatcatc atcgaatgaa tggtgaaata atttccctga ataactgtag
    10081 tgttttcagg gcgcggcata ataatcagcc agtggggcag tgtctacgat cttttgaggg
    10141 gaaaatgaaa attttccccg gtttccggta tcagacctga gtggcgctaa ccatccggcg
    10201 caggcaggcg atttgcagta cggctggaat tgtcacgcga taggcactgc cgctgaccgc
    10261 tttaacccca tttaatgctg cgcccacagg gcctcccaga cccgcgccgc gcagcaaacc
    10321 atgcccaagc acgctcattg ctgcgtgggt gcgtaaaatg cgggtcaatt ggctggaaag
    10381 caaatgcgac acaccttttg ccaataattt gtctttcatc agcagcggca gcagctcttc
    10441 cagctcattc accctggcat cgaccgcgtg cagaaactcc tgcttatgtt cctcgtccat
    10501 tttcttccag gtattacgca gaaattgttc cagtaactgt tgctcaattt caaacgtaga
    10561 catctctttg tcggctttca gcttcaatcg ctttgaaaca tcgagcaaaa tggcgcgata
    10621 caatttaccg tgtccgcgca gtttgttggc gatactatcg ccaccaaaat gctgtaattc
    10681 tccggcaatc agctgccagt tgcggcggtg tcgctcggga tgtccttcca tcgatttaaa
    10741 cagttcgttg cgcatcagta cgctggagag gcgagttttg cctttttcat tatgggtgag
    10801 cagccgggcg aaatttgcca actgttcctc actacaatgc tggagaaaat ccagatctga
    10861 atcattcagg taattaacat tcattttttg tggtttctat attctggcgt tagtcgtcgc
    10921 cgataatttt cagcgtggcc atatccgatg agttcactgt atgaccggaa aaggtgattt
    10981 ttgagacgca gcgtttgttg tcgttatcgc tgttaatgtt gatccagtca gtggtttgcc
    11041 cttcttttat ttctgaagga atattcaggg tctgactggc gcttcgggtg gctttgaaat
    11101 aaatcgatgc accgcttaac tgcaaatcgc catggtcggc tgagagttgt atgcgtttaa
    11161 caatgcgaca aacaggaagt ttcagcgcca gatcgttggt ttcgtttcgc ggcattgcaa
    11221 tgacgccgag gattttatgg tcgtttgcct gcgccgtgca gcacagcatc aggctaatcg
    11281 ccaggctggc ggaaatcgta ataacggatt tcataaggat tctcttggtg gggaggggta
    11341 aggggatgaa actagtttac cgctgataaa gagaagtttc aggcacgtaa tattttcttt
    11401 ttattacaat tttttgatga atgccttgct gcgattcatt ctttatatga ataaaattgc
    11461 tgtcagtttc acgtcttgtc ctgccatatc gcgaaatttc tgcgcaaaag cacaaaaaat
    11521 ttttgcatct cccccttgat gacgtgggtt acgaccccat ttagtagtca accgcagtga
    11581 gtgagtctgc aaaaaaatga aattgggcag ttgaaaccag acgtttcgcc cctattacag
    11641 actcacaacc acatgatgac cgaatatata gtggagacgt ttagatgggt aaaataattg
    11701 gtatcgacct gggtactacc aactcttgtg tagcgattat ggatggcacc actcctcgtg
    11761 tactggagaa cgccgaaggc gatcgcacca cgccttctat cattgcctat acccaggatg
    11821 gtgaaactct ggttggtcag ccggctaaac gtcaggcagt gacgaacccg caaaacaccc
    11881 tgtttgcgat taaacgcctg attggccgcc gcttccagga cgaagaagta cagcgtgatg
    11941 tttccatcat gccgttcaaa attattgctg ctgataacgg cgacgcatgg gtcgaagtta
    12001 aaggccagaa aatggcaccg ccgcagatct ctgctgaagt gctgaaaaaa atgaagaaaa
    12061 ccgctgaaga ttacctgggt gaaccggtaa ctgaagctgt tattaccgta ccggcatact
    12121 ttaacgatgc tcagcgtcag gcaaccaaag acgcaggccg tatcgctggt ctggaagtaa
    12181 aacgtatcat caacgaaccg accgcagctg cgctggctta cggtctggac aaaggtactg
    12241 gcaaccgtac tatcgcggtt tatgacctgg gtggtggtac tttcgatatt tccattatcg
    12301 aaatcgacga agttgacggc gaaaaaacct tcgaagttct ggcaaccaac ggtgataccc
    12361 acctgggtgg tgaagacttc gacagccgtc tgatcaacta tctggttgaa gaattcaaga
    12421 aagatcaggg cattgacctg cgcaacgatc cgctggcaat gcagcgcctg aaagaagcgg
    12481 cagaaaaagc gaaaatcgaa ctgtcttccg ctcagcagac cgacgttaac ctgccgtaca
    12541 tcactgcaga cgcgaccggt ccgaaacaca tgaacatcaa agtgactcgt gcgaaactgg
    12601 aaagcctggt tgaagatctg gtaaaccgtt ccattgagcc gctgaaagtt gcactgcagg
    12661 acgctggcct gtccgtatct gatatcgacg acgttatcct cgttggtggt cagactcgta
    12721 tgccaatggt tcagaagaaa gttgctgaat tctttggtaa agagccgcgt aaagatgtta
    12781 acccggacga agctgtagcc atcggtgctg ctgttcaggg tggtgttctg actggtgacg
    12841 taaaagacgt actgctgctg gacgttaccc cgctgtctct gggtatcgaa accatgggcg
    12901 gtgtgatgac cacgctgatc gcgaaaaaca ccactatccc gaccaagcac agccaggtgt
    12961 tctctaccgc tgaagacaac cagtctgcgg taaccatcca tgtgctgcag ggtgaacgta
    13021 aacgtgcggc tgataacaaa tctctgggtc agttcaacct ggatggtatc aacccggcac
    13081 cgcgcggcat gccgcagatc gaagttacct tcgatatcga tgctgacggt atcctgcacg
    13141 tttccgcgaa agacaaaaac agcggtaaag agcagaagat cactatcaag gcttcttctg
    13201 gtctgaacga agatgaaatc cagaaaatgg tacgcgacgc agaagctaac gccgaagctg
    13261 accgtaagtt tgaagagctg gtacagactc gcaaccaggg cgaccatctg ctgcacagca
    13321 cccgtaagca ggttgaagaa gcaggcgaca aactgccggc tgacgacaaa actgctatcg
    13381 agtctgcgct gactgcactg gaaactgctc tgaaaggtga agacaaagcc gctatcgaag
    13441 cgaaaatgca ggaactggca caggtttccc agaaactgat ggaaatcgcc cagcagcaac
    13501 atgcccagca gcagactgcc ggtgctgatg cttctgcaaa caacgcgaaa gatgacgatg
    13561 ttgtcgacgc tgaatttgaa gaagtcaaag acaaaaaata atcgccctat aaacgggtaa
    13621 ttatactgac acgggcgaag gggaatttcc tctccgcccg tgcattcatc taggggcaaa
    13681 tcaaaaaaga tggctaagca agattattac gagattttag gcgtttccaa aacagcggaa
    13741 gagcgtgaaa tcaaaaaggc ctacaaacgc ctggccatga aataccaccc ggaccgtaac
    13801 cagggtgaca aagaggccga ggcgaaattt aaagagatca aggaagctta tgaagttctg
    13861 accgactcgc aaaaacgtgc ggcatacgat cagtatggtc atgctgcgtt tgagcaaggc
    13921 ggcatgggcg gcggcggttt tggcggcggc gcagacttca gcgatatttt tggtgacgtt
    13981 ttcggcgata tttttggcgg cggacgtggt cgtcaacgtg cggcgcgcgg tgctgattta
    14041 cgctataaca tggagctcac cctcgaagaa gctgtacgtg gcgtgaccaa agagatccgc
    14101 attccgactc tggaagagtg tgacgtttgc cacggtagcg gtgcaaaacc aggtacacag
    14161 ccgcagacct gtccgacctg tcatggttct ggccaggtgc agatgcgcca gggtttcttt
    14221 gccgtgcagc agacctgtcc acactgtcag ggccgcggta cgctgatcaa agatccgtgc
    14281 aacaaatgtc atggtcatgg tcgtgttgag cgcagcaaaa cgctgtccgt taaaatcccg
    14341 gcaggggtgg acactggaga ccgcatccgt cttgcgggcg aaggtgaagc gggcgaacac
    14401 ggcgcaccgg caggcgacct gtacgttcag gttcaggtta aacagcaccc gattttcgag
    14461 cgtgaaggca acaacctgta ttgcgaagtc ccgatcaact tcgctatggc ggcgctgggc
    14521 ggcgaaatcg aagtgccgac ccttgatggt cgcgttaaac tgaaagttcc tggcgaaacc
    14581 cagaccggta agctgttccg tatgcgtggt aaaggcgtca agtctgtccg cggcggcgca
    14641 cagggtgatt tgctgtgccg cgttgtcgtc gaaacaccgg taggcctgaa cgagaagcag
    14701 aaacagctgc tgcaagagct gcaagaaagc tttggtggcc caaccggcga gcacaacagc
    14761 ccgcgctcaa agagcttctt tgatggtgtg aagaagtttt tcgacgacct gactcgctaa
    14821 cctccccaaa agcctgcccg tgggcaggcc aggtaaaaaa aagggtgcgt tgaagatatg
    14881 cgagcacctg ta
//
LOCUS       DAOFSV010000111        14842 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.111, whole genome shotgun sequence.
ACCESSION   DAOFSV010000111 DAOFSV010000000
VERSION     DAOFSV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.111"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(29..2701)
                     /gene="infB"
                     /locus_tag="QBH27_003456"
     CDS             complement(29..2701)
                     /gene="infB"
                     /locus_tag="QBH27_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDQ7015732.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(2726..4213)
                     /gene="nusA"
                     /locus_tag="QBH27_003457"
     CDS             complement(2726..4213)
                     /gene="nusA"
                     /locus_tag="QBH27_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDQ7015733.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(4241..4693)
                     /gene="rimP"
                     /locus_tag="QBH27_003458"
     CDS             complement(4241..4693)
                     /gene="rimP"
                     /locus_tag="QBH27_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDQ7015734.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(4900..4976)
                     /locus_tag="QBH27_003459"
     tRNA            complement(4900..4976)
                     /locus_tag="QBH27_003459"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4940..4942),aa:Met,seq:cat)
     gene            5324..6667
                     /gene="argG"
                     /locus_tag="QBH27_003460"
     CDS             5324..6667
                     /gene="argG"
                     /locus_tag="QBH27_003460"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDQ7015735.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(6675..8300)
                     /locus_tag="QBH27_003461"
     CDS             complement(6675..8300)
                     /locus_tag="QBH27_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDQ7015736.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIIFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRIPSLPLRLLIAAPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTHSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYIINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(8757..8843)
                     /locus_tag="QBH27_003462"
     tRNA            complement(8757..8843)
                     /locus_tag="QBH27_003462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8807..8809),aa:Leu,seq:gag)
     gene            complement(8858..9190)
                     /gene="secG"
                     /locus_tag="QBH27_003463"
     CDS             complement(8858..9190)
                     /gene="secG"
                     /locus_tag="QBH27_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDQ7015737.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(9418..10755)
                     /gene="glmM"
                     /locus_tag="QBH27_003464"
     CDS             complement(9418..10755)
                     /gene="glmM"
                     /locus_tag="QBH27_003464"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDQ7015738.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(10748..11596)
                     /gene="folP"
                     /locus_tag="QBH27_003465"
     CDS             complement(10748..11596)
                     /gene="folP"
                     /locus_tag="QBH27_003465"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDQ7015739.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVCVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(11686..13620)
                     /gene="ftsH"
                     /locus_tag="QBH27_003466"
     CDS             complement(11686..13620)
                     /gene="ftsH"
                     /locus_tag="QBH27_003466"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDQ7015740.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(13720..14349)
                     /gene="rlmE"
                     /locus_tag="QBH27_003467"
     CDS             complement(13720..14349)
                     /gene="rlmE"
                     /locus_tag="QBH27_003467"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDQ7015741.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            14475..14768
                     /gene="yhbY"
                     /locus_tag="QBH27_003468"
     CDS             14475..14768
                     /gene="yhbY"
                     /locus_tag="QBH27_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDQ7015742.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
BASE COUNT         3662 a         4011 c         3437 g         3732 t
ORIGIN      
        1 ccttatcccg catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt
       61 cgaatacttc gatcacatcg ccagtgcgga catcgttgta gttcttaacg ccgataccac
      121 attccatacc gttacggact tcgttaacgt catctttgaa gcgtcgcagg gactccagct
      181 cgccttcgta gataaccacg ttgtcgcgca gaacgcggat cgggttgtga cgtttaacca
      241 caccttcggt aaccatacaa cctgcgatag caccaaattt cggtgatttg aacacgtcac
      301 gaacttccgc cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg
      361 ccgctttcac ttcgtcgatc aggttataga tgacggagta gtaacgcaga tccaggcttt
      421 ccgcttcaat cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg
      481 cgttggacgc cgcagccagg gtagcgtcgg tttcggtgat accacctacg ccagaaccga
      541 tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcagaa attgcttcca
      601 cagaaccctg tacgtctgcc ttcaggacga tattcacttc gtgaacttcg ccttcggtca
      661 tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga
      721 atttaccctg acgataaagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa
      781 cttcatcacc cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac
      841 ccgcttccag cacttcctga cccagttcgt tacgcattgc acgaacacga ccgtattcga
      901 agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa
      961 ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac
     1021 gtaccgcttt cagctccaga acttccgcct gcagcaggat agcatccagc agttcatcaa
     1081 taccggtacc cgcttttgca gatacgtgta cgaactggct ttcgccgccc cactcttctg
     1141 gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcggct tctggtttat
     1201 cgattttgtt cacagcaacc accaccggaa cacccgccgc tttcgcgtgc tggatagctt
     1261 cgatggtctg cggcattaca ccgtcgtctg cagcaacaac caggactacg atgtccgttg
     1321 cctgcgcacc acgtgcacgc attgaagtaa acgcggcgtg ccccggggtg tccaggaagg
     1381 tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg
     1441 cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt
     1501 caacgtgacc catgatggtc acaaccggcg cgcgcggttc agccgcagca cccgtgtcac
     1561 ggtcgctcat taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca
     1621 tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg
     1681 cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca
     1741 gttcgccaac ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct
     1801 tctggaagcc ttgctgcagc gaagaacctt tacgttttcc gcccttaccg ccacgtactg
     1861 ctgcacgagc ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac
     1921 gcgctgcttt cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt
     1981 cttctgcctg gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg
     2041 gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc
     2101 gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc ggctttacgc ttgagctctg
     2161 cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt
     2221 gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct
     2281 tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt
     2341 ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gctgcaaggc
     2401 gctcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact tcgatttgta
     2461 ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca
     2521 gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa gtctgtttct
     2581 cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca aattgctgta
     2641 ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt acatctgtca
     2701 tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa atattacggg
     2761 cagccataat cagtgctccg gctttttcgt cggtcaaccc ttcgatatca gccagatcat
     2821 caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc agtttgaatg
     2881 ccaaatcacg atctacccct tcaaggttca gcagatcatc agccggtttg ttatcaccga
     2941 ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg
     3001 cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc ggcacatagg
     3061 ccagttcttc cagcgtcgag aagccttctt ctaccagaac agtcgcgaag tcttcatcga
     3121 tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct
     3181 gcaggtcgtc aacggtcatc acgttgagtt cccaaccgct cagctgcgaa gccagacgca
     3241 cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca
     3301 tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc attgcgttaa
     3361 tcacgaactg cgccgggtta tcatcccaca ggacgatatc gatacgctct ccgcccagtt
     3421 cagtagaaac cgcctgaaca cgcgcgccac gcatacctac gcaagcacct accggatcga
     3481 tacgtttatc gttggttttc accgcaattt tcgcacgaga tcccgggtcg cgagccgctg
     3541 ctttaatttc aatcacttct tcgccgattt ccggcacttc aatacggaac agttcgatca
     3601 gcatttccgg cttggagcgg gtgacgaaca gctgcgcgcc acgcgcttcc gggcgaacgg
     3661 aatagagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc agcatatctt
     3721 cgcgcaggat cacggcttca gcgttgttgc ccagatccag agagatgttg tcgcggttta
     3781 cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga tcaaccacca
     3841 tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc tgggtagtga
     3901 tacgatcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccaag ttcaggcttt
     3961 catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg acttcatcaa
     4021 caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc tgtacgcgaa
     4081 cgtcgatttc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt
     4141 cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact acagccaaaa
     4201 tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg ggaaccaggt
     4261 tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg gtaactgtga
     4321 tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt tgtaccgcca
     4381 tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca gccgtgaaca
     4441 gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg atggggtctt
     4501 caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca acattgatgc
     4561 catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata aattcgatgc
     4621 caaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt aatttttgct
     4681 ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc agttattctg
     4741 tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac cctataaccg
     4801 caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac gaaggccgaa
     4861 gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg gccggatttg
     4921 aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgcctg
     4981 aaacgtggca aattctactc gttttgggta aaaaatgcaa atactgctgg gatttggtgt
     5041 accgagacgg gacgtaaaat ctgcgggcat tatagtgatc cacgccacat tttgtcaacg
     5101 tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa tacttttacc
     5161 ttctcctgct ttcccttaag cgcattattt tacaaaaaac acactaaact cttcctgtct
     5221 ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca gtgagagcag
     5281 aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga cgattctcaa
     5341 gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg acaccagtgc
     5401 cgcactgctg tggatgcgac aaaagggagc tgttccttat gcatatactg caaacctggg
     5461 ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat acggcgcgga
     5521 gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg ccgctattca
     5581 gtgtggcgca tttcataaca ccactggtgg actgacctat ttcaacacga cgccgctggg
     5641 ccgcgccgtg accggcacca tgctggttgc tgctatgaaa gaagatggcg tgaatatctg
     5701 gggtgacggc agcacctata aaggaaacga tatcgaacgt ttctaccgtt acggtctgct
     5761 gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta ttgatgaact
     5821 gggcggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact acaaaatgtc
     5881 tgtcgaaaaa gcttactcca cggactccaa catgcttggt gcaacgcatg aagcgaagga
     5941 tctggaatac ctcaactcca gcgtcaaaat cgtcaaccca attatgggcg tgaagttttg
     6001 ggatgagagc gtgaaaatcc cggcagaaga agtcacagta cgctttgagc aaggtcatcc
     6061 ggtggcgctg aacggtaaaa cctttagcga cgacgtagaa atgatgctgg aagctaaccg
     6121 catcggcggt cgtcacggcc tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc
     6181 aaaaagccgc ggcatttatg aagcccctgg gatggcactg ctgcacattg cgtatgaacg
     6241 tttgttgacc ggtattcaca acgaagacac cattgagcag tatcacgcgc atggtcgtca
     6301 gttgggccgt ctgctgtacc agggacgttg gtttgactcc caggcgctga tgctgcggga
     6361 ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttactctgg agctgcgccg
     6421 cgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca agccagagcg
     6481 tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtatcg gtcaactgac
     6541 catgcgtaac ctggatatca ccgatacccg cgagaaactt ttcggttatg ccaaaactgg
     6601 cctgctctcc tcctctgccg cttcaggggt gccgcaggtg gagaatctgg aaaataaagg
     6661 ccagtaattc gacatcaacc ctgcccttca ggggtggggt taccttgatt atcctctgta
     6721 aactcctttc tgagcatcac ataatcgaaa acatcatggt ttgcatccac aacccgcgag
     6781 tctcctttat agtgtgcaat cacttcctca agcgtttgcg gctgttccgg ctttgttacc
     6841 cccatccacg cattaataat gtaattattg taagctgtcg aaaagatgtt gttttccgtt
     6901 gtgcgatcca cgccatcccc aagaacgggg ctataccaga taaacatagg gacatgatat
     6961 gcctgctggc tagcctccct gcctccatga aaatagacgt tcttcttcgt agggtcgcgt
     7021 tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa taattcaaaa
     7081 acctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc atcctgatcg
     7141 tcctgcggtt gaaacacagc gctggattgc ggataggcgc tacaagccgg ttcatggctt
     7201 ccatttaaat gaagaacaat cagtttcttt tgctgcgtct tttgctgtaa cgcttgcgat
     7261 aaatgcggca acaacaattc atcaaatcct ctgacataga ctgtttccat ggcgcgcatg
     7321 gcgatactgg taactgctgt accattctgc cgaaaagcgg attgcgagct tagccagaaa
     7381 gtctgaaatc ctgcttgatt agccatatta ataatgttgt ccggataatt atgaatgtca
     7441 tgactcaaaa cagaatcagc agttaaagaa aggggaaccg acagcgcagt gtaaggtgca
     7501 ccgcttattg cttgattaaa cagtttgatc tgctttcttt gtgcttcgac ttgcggtgtc
     7561 gtagagtgtg tatatccata caaagacata ttgtcgacac gtacagactc ccccacaatc
     7621 aacacgtagg tatcaatacc tgtatccctg actgataatt gaaaatacgg caccgtgttt
     7681 gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaataatt gagattgaaa
     7741 aacggcgtat aggtagcaaa tcgcgacgct agtatatatg gactgaacgc ctctttattt
     7801 tttgcatctt tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat gacaatcagc
     7861 aataatattc ctgtcacttt ttttgtcggc aaggaaacat catattttat tattactgcc
     7921 aaaaaaagaa gggataaaaa ggcaaaagca catagataag ggatatacat tcccagcatt
     7981 ttgacaactt catccggatc actctgcagc acactaatcg caaatccatc attgaaagtt
     8041 gtaccaaaga tacaccatga atagaggcta atactcatat ctgccgcagt cagtaacaca
     8101 aatggtgcgg caataagcag gcgaagcgga agggatggta tacgtttaac aaccaggatg
     8161 gtcagaacga agaacagcaa tcgctgaacc atatgcgctc cggaagaagc gactaagttc
     8221 gtaataccaa aaataataac acaaagatac aacaaccaat gagatttaaa acttttaaca
     8281 aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa caattatcaa
     8341 caatgttcaa ttttatatca gacatgtttt tcaactctaa tccatatcag accgaaacac
     8401 tgctagattt tttcttttta aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt
     8461 atcaatgcgt ctctaaacat tacgcagaac gtagccatca gtagttatac tgacaggggc
     8521 aggatggttt atcgagaagt aaacatatcc gtattcgtac cggaaatgga gttgacacaa
     8581 taaagtgcca attatgtcag cagaagggaa aagcttataa ctaaggcata ccggcaccat
     8641 cgtttcaagg taccagctac gagtaaaaca actggacgag atacagatac ctgacaacca
     8701 ttcctcagac caggaccaaa acgaaaaaag acgcttttca gcgtctttct ggaatttggt
     8761 accgaggacg ggacttgaac ccgtaagccc tattgggcac taccacctca aggtagcgtg
     8821 tctaccaatt ccaccacctc ggcacggata ctacttttta gttcgggata tcgctggtcg
     8881 gcttagccgg agcagctggc tgagtttgtt cggttttcgc cggcgcactc aggttttccc
     8941 attcgctacc tttattggtt ttgttgctat tgatgttacc cagcaccaga ctgatgatga
     9001 agaataacgt tgccagcagc gccgtcatgc gagtcatgaa gttaccagaa ccacttgaac
     9061 caaacagcgt agcggaagcg cctgctccga aggaggctcc catatcagcg cctttacctt
     9121 gctgcagcat gatcagacca acaaggccaa ttgccacaat aaggaaaact actaaaagag
     9181 cttcgtacat aatcaacctg ttccttgcgg agttgccgcg taccaatgct tcaaccaata
     9241 aagcgggaat atttaacctt tcccactgaa gcgggtgtga atactaacca aagcgaatga
     9301 ccttcgcaag ggcaatttta ttgcattgta tcaactgcgg aaaaaaacag caaaaccgat
     9361 gtgttcgctg agaaaaaagg cgacaatcgc cgccttttta gccagccatt tatcgtttta
     9421 aacggctttt actgcatcgg cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc
     9481 gccttccacc atcacgcgaa ttaacggttc ggtgccggat ttacgcaaca gcacacgtcc
     9541 acggctaccc agcgccgctt caacttctgc ggtcacagct ttgactgatt catgctccaa
     9601 cggatcgccg ctacctgcgg tgtaacgcac attaaccaga atctgcggga acattttcat
     9661 gccgctgcaa aggtcgtgca ggctcatatg gttacgcgcc atcgccgcca gcacctgcaa
     9721 gccagcaaca atgccgtcac cggtagtggt tttatccagc aggatcacat gaccggagtt
     9781 ctctgcaccg atacgccagc ctttctcctg cattttttcc agtacgtagc ggtcacccac
     9841 tttcgcgcgc gcaaatggaa tacctaactg tttcagcgcc agttcaagcc ccatgttgct
     9901 catcaatgta cccacagcac caccacgcag ctggccctga cgaagacctt cacgcgcgat
     9961 gatatacatg atctgatcgc catcgacttt attgccttca tggtcaacca taatcacgcg
    10021 atcgccgtcg ccgtcgaagg caataccgag atccgctttt tcagccagca cacgagcctg
    10081 gagcgcgcga acgtcggtag cccccacttc ggcattgata tttacaccgt tcggctcaca
    10141 accgatagcg ataacgttcg cccccagttc acgcagcacg ttcggtgcga tgtgataagt
    10201 cgcaccgttt gcacaatcca ccacaatctt cagttcactg aggctaagtt cgttcgggaa
    10261 cgtggctttg caaaactcga tataacgacc cgcggcatca acgatacggc tggctttacc
    10321 cagttctgcc gaatcaacac agctgatctc tttttccatt tccgcttcga tggcttcttc
    10381 aactgcatcc ggcagcttag taccgtcaat agaaaagaat ttaatgccgt tatcgtagaa
    10441 tgggttatgc gatgcagaaa ttacaattcc agcctctgcg cggaaggtac gcgtcagata
    10501 agccacggcc ggtgttggca tcgggccagt gaagagcgcg gaaagaccgg cagccgcaag
    10561 acccgcttcc agtgctgact ccagcatata gccagaaata cgcgtgtctt taccaataat
    10621 aatcttacga gagccgtggc gcgccagcac tttacccgcg gcccaaccca gcttaagcac
    10681 aaaatcaggt gtgatcggcg catcccctac acgaccacga atcccatcgg taccgaaata
    10741 tttacgatta ctcatagcgt ttgttttcct ttgcagacag agtggcttcc accacccgca
    10801 tcgcttctac ggtttctttg acgtcatgaa cacgaatgat gtgcgcgcct tgcattgcgg
    10861 caatgaccgc acaggccaga ctaccgctca ggcgctcgga cggccccacg ttcagcaatt
    10921 gcccaatcat cgattttcgt gacataccca ccaacagcgg caggttgaaa tggtgaaatt
    10981 cagccaggcg cgccagtaat gaatagttat gggagagatt tttaccgaaa ccgaatccgg
    11041 ggtcgagcaa caatttctct tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa
    11101 taaagtagcg attcacttct gcaaagacat cgtcatactt cggcgcttcc tgcatggttt
    11161 ttggatttcc ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca gccgcctcca
    11221 gagcaccagg ttcggaaagg gagcggatat cgttaataat gtgagcgcca actttcgctg
    11281 actcacggat gacttctggt ttggatgtat ctaccgagat ccagacttcg aagcgttgag
    11341 caattgcctc aaccacagga ataacacgtt gcaactcttc ttcaacgcaa acttccgccg
    11401 cccctgggcg cgtggactcg ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca
    11461 gattcgcatg tttcaccgca tctaacagcg agttatgcgt gccaccatcc gaaaaggaat
    11521 caggcgtgac gttgaggatc cccattacgt gaggatggct aaggtccagt gaagtaccct
    11581 gggcaaagag tttcatgatg ttatccctgg tatgaattga taagaaaaaa ccccggagca
    11641 cgccccgggg ttttcggtac aaatacagtc atctgatacg ggaacttact tgtcgcctaa
    11701 ctgctctgac atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc
    11761 ctttggacta ccattgtcgc cagaattgtt agaagcgcct ggttcttccc agcccgctgg
    11821 cggacgtaca tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata
    11881 tttcatgaga gcatctttca tcgcatgcag aatatccata ttgtcggtca gaagctgacg
    11941 cgcacgatta tagttacgct caatcagtgc tttcacttcc tggtcgatga tacgtgcagt
    12001 ttcatcggac atatgtttcg ctttcgctac gctacggccg aggaacactt caccttcttc
    12061 ttccgcgtac agcagtggac ccaatttctc agagaagccc cactgggtca ccatgttacg
    12121 tgccaggttg gtcgcaactt taatatcgtt ggacgccccg gtagaaacat gttccggtcc
    12181 gtagatgatc tcttccgcca gacgaccacc gtacagcgtg gagatctgac tttccagttt
    12241 ctgacggctg gcgctgattg cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg
    12301 accgcgtggg ataatcgtca ctttgtgcac cggatcgtgt tccggcacca ggcgaccgat
    12361 aatcgcatga cccgcctcgt ggtaagccgt cgattctttc tgcgcttccg tcatcaccat
    12421 ggagcgacgt tccgcaccca tcatgatttt gtctttcgct ttctcgaact caaccatcga
    12481 aacaacgcgt ttgttgccac gagcagcgaa cagtgccgct tcgttcacca ggttcgccag
    12541 gtcagcaccg gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc
    12601 caatggtacg cgacgcatgt gaactttcag gatctgctca cgaccgcgaa catctggcaa
    12661 gccaaccaca acctgacggt cgaaacggcc aggacgcagc agggctgggt cgagaacgtc
    12721 cggacggtta gtcgcagcga taacgatgat accttcgtta ccttcaaaac catccatctc
    12781 aaccagcatc tggttcagag tctgttcacg ttcatcgtga ccaccgccca agcctgcgcc
    12841 gcgctggcgg cctacggcgt cgatttcatc gataaagatg atgcacggtg ccgctttctt
    12901 cgcctgttcg aacatgtcac gaacacggga tgcacccaca ccgacgaaca tttctacgaa
    12961 gtcagaaccg gagatagtaa agaacggaac tttcgcttcg cctgcaattg ctttcgccag
    13021 caacgtttta ccggtacccg gaggacctac catcaggacg cctttcggga tcttaccgcc
    13081 gagtttctgg aagcggctcg gctcacgcag atactcaacc agttcagcaa cttcttcttt
    13141 tgcttcgtcg cagcccgcaa cgtcagcaaa ggtggttttg atctgatctt ccgtcagcat
    13201 gcgcgctttg ctcttaccaa acgacatggc acctttgcca ccgccgccct gcatttgacg
    13261 catgaagaag atccagacac caatcagcaa cagcatcggg aaccaggaga tgaagataga
    13321 agccagcagg cttggttctt caggcggttc accgacaacc ttgacgttct tggtcaacag
    13381 gttatccagt aatttcggat cctgaaccgg aatgtaagtg gtataacggt tactatcttt
    13441 cttggtaacg ttgatttcac gtccgttgat acgcgcttca cgaacctggt cgttattgac
    13501 ctcttgtagg aaggtagagt aatccacctt acggccatta gactcgctgg gcccaaagct
    13561 ctggaatact gacatcagca caacggcaat gaccagccag agtattaggt ttttcgccat
    13621 gtcactcaag ggattaacct cttattacaa ctgtgttaaa aacagcgtca ggatactcta
    13681 tatccagcat ctttcaaact ttcgtctgaa atctcccggt tagggtttac gcccggtcgc
    13741 tacaatatac acttcccgcg aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt
    13801 cgtaaacagg gagcgaattt cccttagata ctcatcgaaa ccttcgccct ggaacacctt
    13861 cactacaaaa ctgccacctg gcgctaatac atcacgacac atttctagcg ccagttccac
    13921 cagatacatg gcacggggga tatccaccgc cggtgttccg ctcatgtttg gtgccatatc
    13981 ggacatgaca acctggactt tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag
    14041 ttcatcacga aaatcgccct gaagaaagtc cacaccaacg ataggatcca taggtaaaag
    14101 atcgcaagcg atgatgcggc ctttgccgcc aatttgggtg accacatatt gtgaccaacc
    14161 acccggagca gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt tgtcactttg
    14221 ctgtatttca tcaagtttaa accaggcacg ggaacgtaac ccctttttct gtgcctgttg
    14281 aacatattta tcgctaaagt gttcctgaag ccagcggctg gagctggcag aacgcttctt
    14341 acctgtcatt taactttccc atggggataa ctcatcgtaa ccaattgcgt aaatttttac
    14401 gcgcctattt ggtgatatat gggagatggc ggtagaatga cccgttttca atcccaacgt
    14461 aagcaaaaaa tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac
    14521 atccgctcaa gccagttgtt ctgcttggca gtaatggttt gaccgaaggg gtgctggccg
    14581 agattgaaca agcgttagag caccatgaac tcatcaaggt gaaaatcgcc accgaagatc
    14641 gcgaaactaa aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac
    14701 aggtcatcgg taaaacgctg gtgctttatc gcccaactaa agaacgtaaa atctcgctgc
    14761 cacgctaaga ttatcctaaa gttacacaca ttcgctgtgt aaaacgaggg gttttccgca
    14821 ggcaggagag caaaatgcca cg
//
LOCUS       DAOFSV010000112        14802 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.112, whole genome shotgun sequence.
ACCESSION   DAOFSV010000112 DAOFSV010000000
VERSION     DAOFSV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.112"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..880)
                     /locus_tag="QBH27_003469"
     CDS             complement(<1..880)
                     /locus_tag="QBH27_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7015743.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTP"
     gene            984..1394
                     /locus_tag="QBH27_003470"
     CDS             984..1394
                     /locus_tag="QBH27_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDQ7015744.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            1387..2076
                     /locus_tag="QBH27_003471"
     CDS             1387..2076
                     /locus_tag="QBH27_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDQ7015745.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(2115..3764)
                     /gene="actP"
                     /locus_tag="QBH27_003472"
     CDS             complement(2115..3764)
                     /gene="actP"
                     /locus_tag="QBH27_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDQ7015746.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(3761..4075)
                     /locus_tag="QBH27_003473"
     CDS             complement(3761..4075)
                     /locus_tag="QBH27_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDQ7015747.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(4285..6243)
                     /gene="acs"
                     /locus_tag="QBH27_003474"
     CDS             complement(4285..6243)
                     /gene="acs"
                     /locus_tag="QBH27_003474"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDQ7015748.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSIDAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLKGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            6635..8071
                     /gene="nrfA"
                     /locus_tag="QBH27_003475"
     CDS             6635..8071
                     /gene="nrfA"
                     /locus_tag="QBH27_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDQ7015749.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            8116..8682
                     /gene="nrfB"
                     /locus_tag="QBH27_003476"
     CDS             8116..8682
                     /gene="nrfB"
                     /locus_tag="QBH27_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDQ7015750.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            8679..9350
                     /gene="nrfC"
                     /locus_tag="QBH27_003477"
     CDS             8679..9350
                     /gene="nrfC"
                     /locus_tag="QBH27_003477"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDQ7015751.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            9347..10303
                     /gene="nrfD"
                     /locus_tag="QBH27_003478"
     CDS             9347..10303
                     /gene="nrfD"
                     /locus_tag="QBH27_003478"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDQ7015752.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            10323..12041
                     /locus_tag="QBH27_003479"
     CDS             10323..12041
                     /locus_tag="QBH27_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDQ7015753.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLIPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLTIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFGLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            12034..12417
                     /gene="nrfF"
                     /locus_tag="QBH27_003480"
     CDS             12034..12417
                     /gene="nrfF"
                     /locus_tag="QBH27_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDQ7015754.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            12414..13010
                     /gene="nrfG"
                     /locus_tag="QBH27_003481"
     CDS             12414..13010
                     /gene="nrfG"
                     /locus_tag="QBH27_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDQ7015755.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            13353..14666
                     /gene="gltP"
                     /locus_tag="QBH27_003482"
     CDS             13353..14666
                     /gene="gltP"
                     /locus_tag="QBH27_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDQ7015756.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREMLGKFDKTADQ"
BASE COUNT         3252 a         3839 c         4120 g         3591 t
ORIGIN      
        1 ctggcgttaa ccagtatcct tcacaacagc tcagccacgc gtgggcagag tgggaaaggt
       61 aaacgccctc acgccaaatc attcctaatt tccagcttag ttcgctttcc agcggtagcc
      121 attttaaggt gtttttatct aatcgctggc aaatgggttc cggcaatatg gctattccga
      181 caccggcctg aaccatcgcg gcgagaaaat cccattgccc gctgcgaact gcaattctcg
      241 gcttaacgcc atgttcatcg aataacttca tcaattgctt acttaaggca aaatcttcgt
      301 tatagataag tagaggatgt tccgccaatg tctccggtga gacggcatca cattcaagcc
      361 atttacctga atgtggcgtc agtacacata gtggatgact aaacagcgat aacgttgtca
      421 ggccgctttc ttctactggc agggctgtca gtgccatatc cagttcaccg ttcatcaccg
      481 cttgttgtac cgtcagacca ccaaactccg aaattttcaa ttctacgccg gggtaacgct
      541 ggcgaaaaag gccaatcggc ccggccatca acatcccaac catcggcggg attcccagcc
      601 gcaaaacgcc ggttttaagc tgattgatat cgctaagctc agcttccagt tgccgaaact
      661 cagccagtaa agccagcccg cgctcaaata cgacgcgacc agtatcagtg agaagcagcc
      721 gacgaccatc gcggatcaac aatatacagt tcagctcatc ttcgagattc ttcagcatct
      781 tgctgatagt tggttgggtg acaaacaact tctccgctgc gcgggtgaag ctttgctggc
      841 gcacgacttc gacaaaataa cgcagcgttc tgatatccat aactattccc tttgactata
      901 cctgcaatga ttttaattca tttcagtcag gggcgagcgc tctctatact taccattctg
      961 ttttattttc aggatatcct ctcatgcccg tggcgcttcg ccgcattacc cctgccgtag
     1021 tgcaatctat ccaggtattt tttcaggttg tgctgtatgc aggtttattt atttttgcgc
     1081 agtatctcgt ctcctggctg catttgccgc tgcctgccaa cctggtgggg atgatcctga
     1141 tgctggcgct gattgtttgt cgcattcttc ctctgcaatg ggttcgagcc ggggcgcgtt
     1201 ggctattggc cgaaatgttg ctgttttttg tccctgccgt ggtggctgtg gtgaactatg
     1261 cacaactatt actggtggat ggctggcgta tttttgctgt gatcgcgctg agtacggtga
     1321 tggtgctggg cacgactgcc tgggtagtgg aaaaagtcta tcgctatgaa atgcgcaggt
     1381 taaaccgtgg ctaattttca gctaagtatg ctctgtctgg caattacgct agttatctat
     1441 tttcttaaca aacgccttta tcgccgcttt cgtaaattgc cattgatgcc tctggtgctg
     1501 acgcctacgc tactggttat gctgctggtt tttggcaata tttcctggca aaactatatt
     1561 ggcgaagcgc actggttact atggctatta ggtccggcca ccatcgcctt tgcggtacca
     1621 gtgtatgaca atcgggcgat cattaagcgt cactggatgt cgttgacggc aggcgtactg
     1681 acggcaacgg tggtggcagt gaccagctca gtgtggctgg cgcgattgtt taccctgcca
     1741 gatgagatcc agcgtagcct ggcggtgcgt tcggtcacta cgccatttgc tttggcagcg
     1801 agccagtcgc tcggcggaca gcctgatctg gtggcgctgt ttgtggtggt caccggtgtg
     1861 tttggcatgg cgattggcga cgtgttgttt ttacgtctct ctattcgcga agggatggcg
     1921 aaaggtgctg gattcggcgc ggcatctcat ggtgccggga ctgcgcggtc gtatgaactg
     1981 ggtcaacaag aaggcgtagt tgcaagcctg gtcatgatgc tttcaggtgt attgatggta
     2041 ttggtttcgc cgctggtggc caggatgatg ttctgaagaa attccccgat caagcgaccg
     2101 gggaagggga gagattaatg cgcgcggccc tgctcaacgc caaagccagt ctgggagcgg
     2161 ataaactgcg cacggaacag ttcacgctcg cgcgcacctt ccgctgaatt atccgttgcc
     2221 gagaagaacc agataccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac
     2281 gggaaaatgg ctttttcgtg accgaggatc tgtacccaaa tcgtcgggcc gaggatcatc
     2341 agcaccaccg cagtaatcag tcctaaccag ccgcccagca ttgcgccacg ggtagtcagt
     2401 ttcgaccagt acatggaaag cagaatgatc gggaagttac agctcgccgc gatggcaaac
     2461 gccagcccca ccataaaggc gatgttctga ttctcaaaca gcacgccgag gataatggca
     2521 atcacaccga ggatcagtac ggtgattttc gatacccgca gctcttcacg ttcggtcgca
     2581 ccttttttga agacgttagc gtacaggtcg tgagacaccg ccgatgcgcc cgccagcgtc
     2641 agacccgcaa ccaccgcaag aatggtggcg aaagcaaccg ctgaaataaa gccgaggaac
     2701 aggttgccgc ccaccgcatt ggccaggtga actgctgcca tgttgttacc accaatcaga
     2761 tggcccgccg cgtctttata ttccggattc gcaccgacca gcatgatcgc gccaaagccg
     2821 ataataaagg tcaggatgta gaagtagccc ataaatccgg tggcgtagaa cacgctctta
     2881 cgtgcttcgc gggcatcgct gaccgtaaag aagcgcatca gaatatgcgg caagcccgcc
     2941 gtaccaaaca tcagtcctag ccccaaagag agcgcggaga taggatcttt caccagcccg
     3001 cccggcttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct gaacagattg
     3061 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
     3121 aacagcacgg ctttaataat ttgtacccag gtggtcgcca gcatgccgcc gaacaggacg
     3181 tacatcatca tcagcacgcc gaccagcact accgcaatgt gatagttaag gccaaacagc
     3241 agctcgatca gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc
     3301 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca
     3361 tcggcaaagg tgtagcgacc gaggttacgc agacgttcgg caatcaggaa cagaatgatc
     3421 ggccagccca ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc
     3481 agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg
     3541 ttctgaaaac cggtgatatt gccgcctgcg gtgtagtagt cattacgcga acgtacgcgt
     3601 tttgacgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
     3661 gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct
     3721 gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
     3781 gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccag
     3841 tgagaacaaa ggagatcaca attaccccga caccgactgg aataccgcgt gtgacgctgg
     3901 tgttcggatt cagcggggtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
     3961 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
     4021 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatcgtacca ttcatcacag
     4081 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
     4141 cgcttgggta acccttgccg aatgtaggcc ggataaggca ttcacgccgc atccggcgct
     4201 tgttgtcgga tgcgatgctg gcgcatctta tccgacctac agccgttgcc agatgtaggt
     4261 cggagcgcat ccgacaattg tgggttacga tggcatcgcg atggcctgct tctcttcaag
     4321 cagcttctcg actacgccag gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc
     4381 gcccgccgca attttgcgca gaatacggcg cataattttg ccggagcggg ttttaggcag
     4441 ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg ccaatctctt tacgcaccca
     4501 gttgcggact tctgcgtaca gttctggtga cggttcctcc ccgtgattaa gcgtgacgta
     4561 ggcgtagatc gcctgacctt taatattgtg cggaataccg accacagcgg cttcggcaat
     4621 tttcggatgc gccaccagcg ccgactcaat ctctgccgtc cccaggcggt gaccggagac
     4681 gttcagcacg tcgtccacgc gcccggtgat ccagtaatag ccatcttcat cacgacgcgc
     4741 gccgtcgccg ctgaaataca tatttttgaa ggtggagaag taggtctgct caaaacgttc
     4801 gtgatcgcca aacagcgtac gcgcctgacc cggccaggag tcggtgatca ccagactgcc
     4861 ttcggtggcc cccttcagcg ggttaccttc gttatcgacc agcgccggtt gcacgccgaa
     4921 gaacggacgt gttgccgaac cggctttcag ctcggtagcg ccaggcagtg gtgtgatcat
     4981 gaaaccgcca gtttcggtct gccaccaggt gtcgaccacc gggcatttct cgttgccgat
     5041 cttcttccag taccactccc acgcttccgg gttaattggc tcgcccacgg aaccgagaat
     5101 gcgcagcgac gaacggtcgg tgccttcgat cgctttatcg ccttccgcca tcagcgcgcg
     5161 aatcgccgtg ggcgcggtat agagaatatt gacctgatgc ttgtccacca cctgcgccat
     5221 acgggcaggc gtcggccagt tcggtacgcc ttcaaacatc agcgtggtcg cgccgcaggc
     5281 cagcgggcca tacagcaaat aactgtgtcc ggtaacccag cccacatcgg cggtgcacca
     5341 gtagatgtcg cccggatgat aatcaaagac atatttaaag gtcagcgccg cgtacaccag
     5401 ataaccgccg gtagtgtgca gcacgccttt tggcttaccg gtagaaccgg aggtatagag
     5461 aataaacagc ggatcttcgg cgttcatctc ttccgcctgg tgctgatcgc tggcttgctc
     5521 aaccaggtcg tgccaccaca ggtcgcgccc ttcctgccag tcaatttttc cgccagtacg
     5581 cttcagtacc accacatgct ctacgctggt gacgttcggg tttttcagcg cgtcatcaac
     5641 gtttttcttc agcggaatac tacgcccggc acgcacacct tcgtcggaag tgatcaccag
     5701 tcgtgaactg gagtcgataa tgcgtccggc aacggcttcc ggcgagaagc cgccgaaaat
     5761 caccgaatgt accgcgccaa tgcgggcgca ggccagcatc gcgaccgcgg cttccggtac
     5821 catcggcata taaatcgcaa ctacgtcacc ttttttaatg cccagttcga gcagggtatt
     5881 ggcgaagcgg cagacgtcgc ggtgcagctc tttatagcta atatgtttgc tctggctggc
     5941 gtcgtcgcct tcccagatga tggcggtacg atcgccgttt tcttgcagat ggcggtcaag
     6001 gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg
     6061 ggcaaaggag gtgtttttca ccttctggta cggtgtgatc cagtcgagaa tttttccctg
     6121 ttcgccccag aaggtatctg gtgcgtcaat agattgttga tacatcgcct cgtactgctg
     6181 agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttgact
     6241 catgcttttg ttctccttgt aggatgttaa taatatgtgg cataagcgtt aaatgtaggg
     6301 gtattggcag ttttgtttag tatttgggcg acagatcacg caaaagtaga attgtgcaaa
     6361 taaacggcag ggtaattttt gaaggtcagc aacaaaagtt gattaattct ttgaggaaca
     6421 tgcagttatg catgttgttg aaaaagagga agataccgac taactctaaa gtggtatttt
     6481 acatgcactt acaattgatt aaagacaaca ttcatagtgt ggttatttgt tacacatagg
     6541 ggcgagcaat gtcatgacag tgtaggtgcg gttactgtcg tgaaaaacaa taaaaaccgc
     6601 cattgcaaca atggctcaat tcggatgaag ccctatgaca aggataaaaa taaacgcacg
     6661 ccgtatcttc agcttattga ttcctttttt ctttttcact tctgttcacg ctgaacaaac
     6721 ggctgctccc gcaacacctg taactgtgga agcgaagaat gaaacctttg ccccgcagca
     6781 tcccgatcaa tatctctcct ggaaagctac ctcggaacag tcagagcgtg ttgacgccct
     6841 ggcggaagat ccacggctgg tgatcctgtg ggcggggtat cccttctcgc gcgattacaa
     6901 caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt gaaaccttgc gtaccggtgc
     6961 gccgaaaaac gctgaagatg gtccgctacc gatggcgtgc tggagttgta aaagcccgga
     7021 tgtggcgcgt ctgatccaga aagacggcga agatggctac ttccacggta aatgggcgcg
     7081 cggcggcccg gaaatcgtca acaacttagg ttgtgccgat tgccataaca ccgcctcacc
     7141 agagttcgcc aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg ctcgcgcgat
     7201 ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat cgatggtttg
     7261 cggtcagtgc catgtggagt attacttcga cggcaaaaac aaagcggtta aattcccgtg
     7321 ggatgacggc atgaaagtcg aaaatatgga gcagtattac gacaaaattg ccttctctga
     7381 ctggactaac tccctgtcga aaacgccaat gctgaaagcg cagcacccgg aatatgaaac
     7441 ctggacagcg ggcattcacg gcaaaaacaa cgtgacctgt atcgattgcc atatgccaaa
     7501 agtgcagaac gccgaaggca aactctacac cgaccataaa attggtaatc cgtttgataa
     7561 cttcgcccag acttgtgcga actgccatac ccaggacaaa gctgccttgc aaaaagtggt
     7621 cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag gttgaagatc aactggttca
     7681 cgctcacttc gaagcgaaag cggcgctgga tgcaggcgca acggaagccg aaatgaagcc
     7741 gattcaggac gatatccgtc atgcccagtg gcgttgggat ttggcgatcg cttcccacgg
     7801 cattcatatg cacgcaccgg aagaaggtct gaggatgctc ggtacggcga tggataaagc
     7861 ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc aaaggcatta cccatgaaat
     7921 ccagatcccg gatatctcga ccaaagagaa agcccagcag gccattggcc tgaacatgga
     7981 gcaaatcaag gccgagaagc aggacttcat caaaacggtg atcccgcagt gggaagagca
     8041 ggcacgtaaa aacggtctgt taagccaata accccgttcc gcctcgcaag gggcggaaaa
     8101 cacaatggag tgaatatgag cgtattacgt tcgttattaa ctgccggggt gctggcgtcg
     8161 ggcctgttgt ggagcctgaa cggaattacc gctacccctg cggcgcaggc atctggtgat
     8221 cgctacgaag ttacccagca gcgtaacccg gatgccgcct gtctggactg tcataagcca
     8281 gataccgaag gtatgcatgg caaacatgct tccgttatca acccgaataa caaactgccg
     8341 gtcacctgca ccaactgtca cggccagcca tcaccgcaac accgcgaagg ggtgaaagat
     8401 gtgatgcgct ttaacgagcc gatgtacaag gttggagagc agaacagcgt ctgtatgtcc
     8461 tgccatctgc cggaacagtt gcaaaaagca ttctggccgc acgatgtcca cgtaaccaaa
     8521 gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag atacgatgca gacgttaagt
     8581 gacaaaggac ggattaagat ttgcgtcgat tgccacagcg atcagcgcac caatccgaac
     8641 tttaacccgg cgtctgttcc gttgcttaag gagcagccat gacctggtct cgtcgccagt
     8701 ttctcaccgg cgtcggtgtg ctggccgccg tcagcggcac cgcagggcgc gtggtggcga
     8761 aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg ttatgcatcg
     8821 gctgtaccgc ctgtatggat gcttgtcgga aagtgaacaa ggtgccggaa ggcgtctcgc
     8881 gcctgacgat tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa tatcgcttct
     8941 tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc ccgaccggtg
     9001 cgtcttttcg cgacgctgcc agtgggattg tcgatgttaa tccggatctc tgcgtcggtt
     9061 gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg gtcacgaaga
     9121 cggcggacaa atgcgatttc tgccgtaaga cccatttgca ggccggtaag ctgcccgcgt
     9181 gcgttgaagc ttgcccgacg aaggcgctga cgtttggcaa tctggacgat cccaacagtg
     9241 agatttcgca actgctgcgc cagaagccca cttaccgcta caagctggcg ctgggcacca
     9301 aaccgaagct gtaccgcgta ccgtttaaat acggggaggt gagccaatga cgcagacttc
     9361 cgcatttcat tttgaatcgc tggtgtggga ctggccgatt gccatctacc tgtttttgat
     9421 tggtatttct gccggtctgg tgacactggc cgtgctgtta cgtcgcttct acccgcaggc
     9481 aggcggtgca gacagtacgt tgctgcgcac cacgctgatt gtcgggcctg gcgcggtgat
     9541 cctcggtctg ttgatcctcg tcttccacct gacaagaccg tggaccttct ggaagctgat
     9601 gttccactac agttttacct cggtgatgtc gatgggggtg atgctgtttc agctttacat
     9661 ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg ccctgcaaca
     9721 acgctggttg ccgaagctgg ggatcgtgca aaaggtcttg agcctgctga cgcccgttca
     9781 tcgcggactg gaaacattga tgctggtgct ggcggtgttg ttgggggctt ataccggctt
     9841 tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc cggtgctgtt
     9901 cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg cgatacgcca
     9961 acgcagtaac ccgcattcca cggaagcgca ctttgtacac cgtatggaaa tccccgtggt
    10021 atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg gcgatgacgg
    10081 taaagtgcgt gcgctggtgg cggcattggg cggcggtttc tggacgtggt ggttctggct
    10141 tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc aaaccgtggg tcaatcgcag
    10201 ttccggcatt cctgccgtgc tggcggcatg tggggccagc ctggtcggcg tgttgatgct
    10261 gcgctttttc attctctacg ccgggcagtt aacggtggcg taagccagaa cagaggtggt
    10321 ttctggacgt attccttcct gaagtcggtt ttctggcgtt gttgttaagt ctcggggtca
    10381 acgtgttgat cccgttgacg gccttcgcgg gagtgcggtt gcgctggcct gccatgatgc
    10441 gactcacttg catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc tttggcgtac
    10501 tgacgtattg ttttctcatc agcgatttct cggttattta tgtcgcccaa catagctata
    10561 gcctgctgtc gtgggaactc aaactggcgg cggtgtgggg cggtcatgaa ggttcgctgt
    10621 tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat taccggcagc
    10681 aaaccgatcc gctatttccg ctgacgctag ccgttttatc tctcatgctc gccgcactgc
    10741 tactgtttgt ggtgctgtgg tccgatccct tcgtgcggat atttccacca gcaatcgaag
    10801 gccgcgatct caatccgatg ctgcaacatc ccggtcttat ctttcatcca ccgctgcttt
    10861 atctcggcta cggcggtttg atggtggcgg cgagcgtggc gctggcgagt ttactgcgcg
    10921 gcgagtttga tggtacctgc gcccgaattt gctggcgttg ggcactacct ggctggagcg
    10981 cattaacagc ggggatcatc ctcggttcct ggtgggccta ttgcgaactg ggctggggcg
    11041 gctggtggtt ctgggatccg gtggaaaacg cctctttact accctggctt tctgccacag
    11101 cgctgctgca cagtttatcc ctgacacgcc agcgggggat tttccgccac tggtcgctgt
    11161 tgctgacgat agttactctg atgctgtcgc tgctgggcac gttgattgtc cgttctggca
    11221 ttctggtttc ggttcatgcg ttcgcgctgg ataacgtccg cgccgtgccg ttgttcggcc
    11281 tgtttgtact gattagcctt gcgtctctgg ctctgtatgg ctggcgagcg cgggacggtg
    11341 gcccggcggt gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct acgctgttgc
    11401 tgttttgcgc agtgctactg atcgtgctgg tgggaacgct ttatccgatg atttacggcc
    11461 tgctgggctg gggacgcctc tccgttggcg cgccgtattt taaccgcgcg acgttaccgt
    11521 ttgctctgtt gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc aaacgcgcgc
    11581 ggcttccgac gctgctggcg catgcgggcg tgctgttatt tgccgcgggg atcgtggttt
    11641 ccagcgtcag ccgtcaggag atcagcctga atttacagcc gggtcagcag gtgacgctgg
    11701 caggatacac cttccgtttt gagcgcctcg atctgcaagc caaaggcaat tacaccagcg
    11761 aaaaagcgat agtggcactg tttgaccatc agcaacgcat tggtgaatta acgccggagc
    11821 ggcgttttta cgaagcacgc cgtcagcaaa tgatggaacc ctcgattcgc tggaacggca
    11881 tccatgactg gtatgcggtc atgggagaga aaaccggagc ggatcgttac gcatttcgtt
    11941 tgtatgtaca aagcggtgtg cgctggatct gggggggagg attgttgatg attgcgggcg
    12001 cattgttaag cggatggcgg ggaaggaagc gcgatgaata aagggcttct cacgctactg
    12061 ctgttattta cctgttttgc gcgcgctcag gttgtagaca cctggcaatt cgctaatccg
    12121 caacaacagg aacaggcgtt aaatattgcc agccagttac gttgtccgca gtgccagaat
    12181 cagaacttac tggaatccaa tgcgccggtg gcagtcagta tgcgccatca ggtttacacc
    12241 atggtggaag aggggaaaag tgaagtcgaa atcattggct ggatgaccga acgctacgga
    12301 gattttgttc gctataaccc accgttaacg ggtcagactc tggtgttgtg ggcgctgcca
    12361 gtagtgttgt tactgctgat ggcactgatc ctctggcgag tgagggcgaa gcgatgaaac
    12421 agccccaaat accggtgaaa atgctgacaa cactcacgat tttgatggta tttctctgtg
    12481 tcggcagtta tctgttaagt ccaaaatggc aggcggtacg tgcggagtat cagcgtcagc
    12541 gcgatccgct gcatcagttt gccagccagc aaaccccgga agcgcagctt caggcattgc
    12601 aggataaaat ccgtgctaat ccacaaaaca gcgaacagtg ggcgttactg ggcgagtatt
    12661 atctgtggca aaacgattac agcaattcgc tgctggcgta ccgtcaggcg ttgcaactac
    12721 gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt gctgtattac caggccagcc
    12781 aacatatgac cgtccaggct cgcgcaatga tcgacaaagc cctcgcgctg gactctaacg
    12841 aaatcaccgc tctgatgctg ctggcttccg atgcgtttat gcaggcgaat tacgcgcaag
    12901 ccatcgaact atggcaaaaa gtgatggatc tcaactcacc ccggatcaac cggacacagc
    12961 tggtcgagtc gattaatatg gcgaaattgt tgcagcggag atcagattaa tcatctggtg
    13021 atgaagatta tgttattgag ctgcataaaa aataatcgaa tgaacatata atcaaaaata
    13081 atcactaatc agtattattg cagattaaac aaataaaaat ccttctataa caaatggtta
    13141 ttcattaatc ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg
    13201 ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta
    13261 caaaccaacg caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg
    13321 gggaacggcg ctccattgag gaagttattc tgatgaaaaa tataaaattc agcctggcct
    13381 ggcagattct gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata
    13441 gcgacagccg cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc
    13501 atctgatcaa aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg
    13561 gtgttggtga tgccaaacag ctcgggcgta ttggtgcgaa aaccattatc tacttcgagg
    13621 tgatcaccac cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg
    13681 ccggggtgga tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg
    13741 aagcggtaca aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga
    13801 acatcgtggc gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt
    13861 ttggtctggg gctttcatcc ctgcccgcga cgcatcgtga accgctggtg accgttttcc
    13921 gctccatctc tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcgccggtgg
    13981 gtgtgtttgc gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac
    14041 tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtcgtactgg
    14101 gaattgtggc gcgcctgtgc gggttaagtg tctggatcct gattcgtatt ctgaaagatg
    14161 agctgattct ggcgtactct actgccagtt ctgaaagcgt gctgccgcga attattgaga
    14221 agatggaagc ctacggtgca ccggcgtcga tcaccagttt cgtggtgccg accggttact
    14281 cttttaacct tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc
    14341 tgtatggcat tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga
    14401 cctcgaaagg gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg
    14461 gtagcgtagg tattccgctg gaaggtctgg cgtttattgc cggtgttgac cgtatcctcg
    14521 atatggcgcg tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca
    14581 agtgggaaca caaatttgac cgtaaaaaag cgctggccta cgagcgtgaa atgttgggca
    14641 aatttgataa aacagcagat cagtaattga agattgccgg ggggatatcc acccggcaat
    14701 gtgtggatgc ctgactgagt aatagcctga tgtagcgtga tttttgtagg tcggataagg
    14761 cgtttacgcc gcatccgaca tcaacgcctg atgcgacgct ta
//
LOCUS       DAOFSV010000113        14782 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.113, whole genome shotgun sequence.
ACCESSION   DAOFSV010000113 DAOFSV010000000
VERSION     DAOFSV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.113"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..122)
                     /locus_tag="QBH27_003483"
     CDS             complement(<1..122)
                     /locus_tag="QBH27_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015757.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISG"
     gene            complement(128..826)
                     /locus_tag="QBH27_003484"
     CDS             complement(128..826)
                     /locus_tag="QBH27_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDQ7015758.1"
                     /translation="MQDIQQETLNECTKTEQSALVVLWEIDLTEVGGDRYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(826..1167)
                     /locus_tag="QBH27_003485"
     CDS             complement(826..1167)
                     /locus_tag="QBH27_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015759.1"
                     /translation="MPESEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVTDEYSRAALDSFLSRHAGIRAFLWRPPKHNRTVRVVCREWSISDNAMYT
                     DFNCTFEEVTH"
     gene            complement(1160..4387)
                     /locus_tag="QBH27_003486"
     CDS             complement(1160..4387)
                     /locus_tag="QBH27_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ7015760.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQSSQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNENGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEVQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLTAAQVSLTRNIAART
                     AAQTTLNNVTSVGSRLLSGALGLVGGVPGLVMLGAAAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEASDNEEKTRQALKEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERR
                     VALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQTALNNSERELALSRLKGEARERARLGYAADDLGFVGEAYQTARLNYINNSLDAW
                     RNNQANKPKAHKKTEAEKIEDTYKQLIKQQKEQIALAGQNTELAKMKYQVSQGELSTL
                     SEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASVRASNEAQLLGYGEGSRMRER
                     LQEMWSIRQEFEQKNNELLRQYQAGEIEEALWKQEKSLNEKYLEERLSDQQDYYAKAD
                     ALRSNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWG
                     SSVLREVSKILMNAAIVNGLKSLSGAGGWLGTVGDWLSGAVANAKGGVYTSANLSAYS
                     NTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGGGFVYSPVY
                     HISIQNKGSNGEIDTQSARGLVDLIDSRVVSIMHSSRRDGGLCSA"
     gene            complement(4434..4712)
                     /locus_tag="QBH27_003487"
     CDS             complement(4434..4712)
                     /locus_tag="QBH27_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDQ7015761.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEKEQTEGVSDPFAGLENALLIVSQ"
     gene            complement(4736..5107)
                     /locus_tag="QBH27_003488"
     CDS             complement(4736..5107)
                     /locus_tag="QBH27_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HDQ7015762.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(5122..5826)
                     /locus_tag="QBH27_003489"
     CDS             complement(5122..5826)
                     /locus_tag="QBH27_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HDQ7015763.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTEPLKVKVDLKSTLRVA
                     SGSKLEMAVEAAGGVPPYSYVWKKGGSPVSGQTAATFSKASAVSGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(5886..6230)
                     /locus_tag="QBH27_003490"
     CDS             complement(5886..6230)
                     /locus_tag="QBH27_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDQ7015764.1"
                     /translation="MREGTLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADDIREQALDAVRKLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(6227..6676)
                     /locus_tag="QBH27_003491"
     CDS             complement(6227..6676)
                     /locus_tag="QBH27_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDQ7015765.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAF
                     YWRFVELGTINMPAHPFIRPAFDTTEELAAQVAIQRMNQAIDEVLSK"
     gene            complement(6673..7011)
                     /locus_tag="QBH27_003492"
     CDS             complement(6673..7011)
                     /locus_tag="QBH27_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDQ7015766.1"
                     /translation="MQAGRLRDRVIILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     REIISGGAETAQATVRVWMRFRRDVTATSRLKVLTGAFKGAILGIEGPPIPDARATRL
                     EILCSLKGNV"
     gene            complement(7020..7337)
                     /locus_tag="QBH27_003493"
     CDS             complement(7020..7337)
                     /locus_tag="QBH27_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDQ7015767.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            complement(7414..8631)
                     /locus_tag="QBH27_003494"
     CDS             complement(7414..8631)
                     /locus_tag="QBH27_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDQ7015768.1"
                     /translation="MKLHEMKQKRNTIAKDMRALHEKIGDNAWTDEQRAEWNRAKAEL
                     DALDEQIAREEELRRQDQAYVDESGPEERQNNEAENGKKAVEEKRAAAFNRFLRAGFA
                     ELNAEERNLMRELRAQSVTTDSQGGYTVPTQMRNKIIDTMKAYGGIASVAQLLTTSTG
                     QDITWSTSDGTTEEGELLAENTAATEQDVTFGTAILGAKKLSSKIIRVSNELLQDSGV
                     DIESYLANRIAQRIGRGEAKYLVQGTGTGSPLQPKGLAASVTGTIQTAASAAFTWKEM
                     NALKHAIDPAYRGGPKYRWAFNDATLQTIEEMEDGQKRPLWLPDIAGGTPATVLGIPY
                     VIDQAIDGIGTGKKFIFLGDFNRFIIRRVTYMELKRLVERYAEFDQVAFLAFHRFDCV
                     LEDVAAIKALTGK"
     gene            complement(8646..9245)
                     /locus_tag="QBH27_003495"
     CDS             complement(8646..9245)
                     /locus_tag="QBH27_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDQ7015769.1"
                     /translation="MNDRETRCYSGEVRAEQYDNAPTHILGYGSVFNSRSEPLWGFRE
                     IIKPGAFDDVLNDDVRGLFNHDPNFILGRSSAGTLSLSVDERGLRYDIVAPDTPTICD
                     LVLSPMLRGDINQSSFAFRVARDGESWYEDDEGIVIREITRISRLYDVSPVTYPAYQD
                     ADSGVRSMKAWQEARASGALKKAVNERMARERLLTLLNA"
     gene            complement(9238..10464)
                     /locus_tag="QBH27_003496"
     CDS             complement(9238..10464)
                     /locus_tag="QBH27_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ7015770.1"
                     /translation="MLLDALFRSEPLENPSVPVTGEAAETDNIFARDVYVSPETSMKL
                     AAVYACIYVISSSVAQMPLHVMRKTNEHVQPARDHPLFWLVHDEPNAWQTSYKWRELK
                     QRHVLGWGNGYTWVKRNRRGEVTSLECCMPWETTLLNTGGRHTYGVYNEEGAFAVSPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQQYTSAFFNGNARPAGIISVKNELNE
                     QSWGRLKNMWQRAVTALRSQENKTMLLPAQLDYRALTVSPVDAQIIDMTKLNRSMIAG
                     IFNVPAHMINDLEKATFSNITQQAIQFVRYTMMPWVANWEQELNRRLFTRTERAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVDGLDEMLVSVNAANP
                     LNNFKDTKGKEEKNDE"
     gene            complement(10454..10615)
                     /locus_tag="QBH27_003497"
     CDS             complement(10454..10615)
                     /locus_tag="QBH27_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015771.1"
                     /translation="MKQLITDMTGLIGFGLLTAGVYLYAGLPASLMLSGCLLLLYALV
                     VSMRRKHAS"
     gene            complement(10612..12369)
                     /locus_tag="QBH27_003498"
     CDS             complement(10612..12369)
                     /locus_tag="QBH27_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ7015772.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEKRGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADSEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPQHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVALFTAMSRFLVNG
                     GGVNDFLSTLDPDEDLLIL"
     gene            complement(12369..12851)
                     /locus_tag="QBH27_003499"
     CDS             complement(12369..12851)
                     /locus_tag="QBH27_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDQ7015773.1"
                     /translation="MSGPPKIPPRLHLIRGNPSKRPVKDLKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRSETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDNVDPLAELLKARD
                     "
     gene            complement(12999..13349)
                     /locus_tag="QBH27_003500"
     CDS             complement(12999..13349)
                     /locus_tag="QBH27_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ7015774.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            complement(13454..13639)
                     /locus_tag="QBH27_003501"
     CDS             complement(13454..13639)
                     /locus_tag="QBH27_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HDQ7015775.1"
                     /translation="MRELKMKLFALTLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWKTPLNGIISPSESG"
     gene            complement(13856..14389)
                     /locus_tag="QBH27_003502"
     CDS             complement(13856..14389)
                     /locus_tag="QBH27_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ7015776.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCEQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIEQ"
     gene            complement(14440..>14782)
                     /locus_tag="QBH27_003503"
     CDS             complement(14440..>14782)
                     /locus_tag="QBH27_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDQ7015777.1"
                     /translation="TQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDRV
                     EGAALEFYEEAARRSIRQVFLDVAAGLCEGGEQSPEKRPVILEAQNVWITYKGKLPGR
                     ITGSLKTPPLG"
BASE COUNT         3418 a         4335 c         3326 g         3703 t
ORIGIN      
        1 ccggagatat tcacgcaggg aaaatatctt tccccctccg gcgttctcac cacgaagccg
       61 cacgactccg ctggcgcaca tcgccgggcg tgcgccagaa tcgctgattc tgtctctgtc
      121 atgggattta ctgcgaaagt ttgttaatgg aaaggaagcc gccaaagttg ccgacgttat
      181 tgcggaactt acagccactc aggcatttgc tgcatttatc cttcgtaata tcggacgtcg
      241 gctgatcata ttcatccgcg accgccggac cgctataacc gcactcatcg ccgcgatagg
      301 tccaggtgca ggtgttggcc agcatgatac gtcccggaaa aacagcgccg tccgtttccg
      361 tcggcgtgga cagtacaaag gaggcactga ccgcgctcag ttcgctgcac tgctcgatgc
      421 gccagcggct gatcacctcc tgctccggat cggcgtcact gtttccgttg acgaagttca
      481 ccgcatccag aaaacgggcg taaaccttac gccggaccac cgttccgccg accagactct
      541 gcagatcttc cgccatcccg gtgaccatgc cgtacagatt agagacttta agcgttggcc
      601 tagcactggc tcctttgccg ttcatctcaa atccgcttcc ctgaatggga taagcctgat
      661 actgccgccc ctgccaggtg actggttcac ctttttcgtt ctgctcatta cagaagaaat
      721 aacgatctcc gccgacctct gtcagatcaa tttcccagag cacgaccagc gcggattgct
      781 ccgttttagt gcactcattg agtgtttcct gctgtatatc ctgcatcagt gagtgacctc
      841 ttcaaaggta cagttaaaat cggtatacat ggcattatcc gaaatgctcc actccctgca
      901 gacaacccgg acagtcctgt tgtgttttgg cggacgccac aaaaaagcac gaatcccggc
      961 atgacgggat aaaaaactgt ccagcgcggc acgggaatat tcatctgtga cacgaaatac
     1021 cggtttaaac gttttcagat ccgcattcag accaccagcc cgtctctgtt catatccgtc
     1081 accgaacttt accgtaataa cagatggctt tcgtgtcgtc tccatcccct cgcgggggat
     1141 ccagttaaaa acttcagact caggcactgc ataatcctcc gtcccgacgt gatgaatgca
     1201 taattgacac aaccctgctg tcgatcagat ccaccagccc cctggctgac tgcgtatcta
     1261 tctcgccatt gctcccttta ttctgaatgc taatgtgata cacgggagaa taaacaaatc
     1321 caccgccacc attcacattg ccaatggctc tgactccaag agagccgtcc gctgcccgag
     1381 tcagtggcat gatagcttca ggccctgcct cgcccatcag cccggcacct ttcgcaaaag
     1441 caaaatacgt cggggtatcc acaatggtgt tactgtaagc actcagattt gcagatgtat
     1501 aaacaccacc ttttgcgttt gccactgcac cggaaagcca gtcgccgacc gtaccaagcc
     1561 accctccggc accggagagt gatttcagtc cgttaacaat ggccgcattc atcaaaattt
     1621 ttgaaacttc ccggagaact gagctacccc agttcctcca gtccacaaca ttcccggcca
     1681 gtgcatcgga aatatttgat accagcccgt ccatagtgga aacgacagca tctgccgcct
     1741 gcgaagcata atcggtggca ctgtctgccc agttcgtcag tccctcctgg agtccggcat
     1801 tccagttact gcgtaaagca tcagcctttg cataataatc ctgctgatcg ctgagacgct
     1861 cttccagata tttttcattc agagattttt cctgtttcca cagggcttct tcaatttctc
     1921 cggcctgata ctgtctcagt agctcgttat ttttctgctc aaactcctgc cggatgctcc
     1981 acatttcctg aagtcgttca cgcatccgtg agccttcacc atatcccagt aactgagcct
     2041 catttgatgc tctgacactg gcattactgt ccgccagact gctctcatac gcagcaagct
     2101 gctcacgaat ctttttctgg tcgatgagtg ctgcattctg caaaagcgtt tttttctgcg
     2161 cttctgacag ggttgataat tcgccctggc tgacctgata tttcatctta gccagttcag
     2221 tattctgccc tgccagtgct atttgctctt tttgctgttt aatcagttgc ttataagtat
     2281 cttctatttt ttccgcttcg gtctttttat gcgctttggg tttatttgcc tggttatttc
     2341 gccatgcatc cagtgagtta tttatataat tcagtcttgc tgtctgatac gcctctccca
     2401 caaagccgag atcatccgca gcataaccca ggcgggcacg ctcacgagct tcccccttca
     2461 ggcgggacag agccagttcg cgctcgctgt tattcagtgc ggtctgctgt ttatcatcca
     2521 gggttgcctg tggtagccgt aacggtacat tcaccagccc ctgtcgctgc tgaagtaatt
     2581 cattaccgag cccgagaagg cgattaaact cggtatgctg cccattcatg atcaacaggg
     2641 actgatacgc tttgttttgt tccgctgcct gttgacggat caacgccacc cgtcgctcct
     2701 ccagcccggc aagcacatcc tgaatggatt gcgctttgcc ctgcatttga gtgaggcgag
     2761 actgttcaac tgccagttga tttgttgctt ctgcaagccc ttctgtgaca gtttttaccg
     2821 acgtcatgtg gttaatcata aaaccgttat cggttgtcca gcccgggttt gccagcacat
     2881 actgatagcc agcaattttt tcctgtaagg atttaatctt acttttctgc tcgtcaatta
     2941 acctgttttg ctccttcagt gcctgtcgcg tcttttcctc attatctgac gcttcaggaa
     3001 gcgacattgc cgacgttttc tggcgaattt cgtcgattgt tgcggcatac tgtcgtgcag
     3061 attctctggc ctgctcctga ttctgataca tcgtgtacca ggccgcagcc cccagcatga
     3121 cgagtcccgg cacaccacca accagcccca gcgcgccact taataaacga ctccccactg
     3181 acgtaacatt attcagcgtt gtctgtgccg ctgttctggc cgcaatatta cgggtaagtg
     3241 acacctgggc agctgtcagc ttcgcttctg ctgcggcctg cctttcggta ccgcgagcag
     3301 caacaaccgc ctgttgcgca cgataaaccg ccgcacgcgc cctggcggtt gctatctgtg
     3361 tcccccgaag ttgcacttca gcaagagcca cttcgtttct ggctgcagta attaatccgg
     3421 cagttgcaga tccagcagac gacgccatat tgccaaaata tcgggctacc ccgacggcaa
     3481 ccagagcacc agcagcggtt gccacggtgt caatattgcc tgcaatacca ttcagcacac
     3541 cggagagcgt cttcgtcact ccgcttgcct cgttcgcacc accaacccag gccataaagg
     3601 cgttttcaac tttggttgca gaggatgaaa ccgtatcagg cattgctgca tattcatcac
     3661 gcaacgcccc aagctgacta atcagtgcag gaacaacctt atcggcggtc agttttccgt
     3721 tatccgccat ggccttcaga tccttacggg caacccccat tcccgcagcc agcgcacgaa
     3781 taacacgatc gccgttctca ttcaccgagt taaactcttc accgcgcagc actccctgcg
     3841 ccagtgcctg actgaactgc gtgatcaccg aactggcttc tgctgtactg gcaccggata
     3901 atttcaggcc cgtggagatc gcctcggtga ctttcagtac ctcctcagaa ctgtaaccat
     3961 actcccgcat ggaagctgca gaacgggcaa aaaggctggc gttatcagaa aacgccgtcc
     4021 ccgttctctg gctgatcgcc attaattcac gctgtgatga ctgaaaatca tcactggact
     4081 gtgaggcctg cttcagacgg gcatttactg aattccactc atcggcgaga gaaataagat
     4141 gaccggtagc aaaagccccg gcaaatgccc ccgccatgtt cagtgccgaa gatttagctg
     4201 tatttatctg atccgtcact tctgccagtg cacgccgcat ttcacgggat gcagcagcgg
     4261 actgtcggcc tccgttctgc atggtacggt agtaatcctg ccccatacgc gaagcccggg
     4321 agatctctga ctggaatgac cgggaatttg ccgagatttt aataatcagt tcacgtaatg
     4381 tcgccacact cattctccgg acgaaaaaaa accgccgaag cggttatgtt gactcactga
     4441 gacactatta aaagcgcgtt ttccagtcca gcaaatggat ctgatacgcc ttctgtctgc
     4501 tccttctccc actgaagaag cgcatcattc agtggcactt tgaccccctg cgcaccgtaa
     4561 acagctgaaa caatctgggc agcccggata tcagcccgtt cgtcccccag cgggctgaac
     4621 ctgtcaaatt ctgcccacat catgatttct gatgcggaca tttcccggcg taactctgac
     4681 aatgtgcgcc ccatcctgag cgccagcatc atcagaaaac gcatccccgg aagcgctact
     4741 tttttttaac ctcgccggca tcactgatta gttccagaga ctgccgaaga agccgcgcat
     4801 gcaccgggcc atacacggca atcacctgtt cacgatcatc ctctgaaaat acaggttgca
     4861 gtccggtatc acacagaaca tcgatgaaca gttcaacatc cgcttccaga tttcggcggg
     4921 cgcgctccgc aacggataac ggtgtctcat catcttttgc tttaacgatc tcctgccagc
     4981 gcaaccaggc ttctgcagaa ggttcccgta atacaaccgt tgccccttcc cattcaggca
     5041 catcaacagt tttatggcga aaccccgaca tcgttgccag tgccagatta cggatatttt
     5101 tagtcatcac atctatcctc attaactgac ggtaacagtg caggaagtag aggtcacttt
     5161 gttaaccggg cttgctgaat cagaaatctc gcaggtatat gcacccgcat caccggatac
     5221 tgccgatgcc ttactgaacg ttgccgccgt ctgtccggaa acaggagaac cacctttctt
     5281 ccagacataa gaataaggcg gcacaccacc cgcagcctca accgccattt caagtttcga
     5341 tccggaagca acccgcagcg tgctttttaa atcgaccttc actttcagcg gctctgtcgt
     5401 cagcacaggt ttacctttca ggcgcagaga aaacgttgca gccacaacac cattggttcc
     5461 tgcagaccag gtatgctgac gcacctctgc cataaaggta aatccgttgc ctgacggaaa
     5521 aataacttta aagccatacg tggtgtcatt gtcataggca ctgcgcaacg cgttctgggc
     5581 agcattgagg taaaagttgc ctgacatgga aatctctgaa gcagcaccaa ggccgttaat
     5641 attttcctgc tcaacagaac acagcgtggt gacatcaata tcctgctttt gtcctgcagt
     5701 aaactgcacc tctttgattg tacagctcag gccaagatag ctggcagaat ccagggtttc
     5761 tgctgttacc ggtgcagacg aaatcataat tttcgtcagt tgcgaacgct caaaattaga
     5821 ggacatactc gtctcctgaa aataaaaaac ccgccagcgg cgggtgggta aaatcattaa
     5881 cgacctcagg ctattacctg aaattcaagc gtggctctgc tcagacggga gtcaggatca
     5941 tacccctgcg ttttagaaat aacggagggt gccagtttcc ttactgcatc aagcgcctgc
     6001 tcacgaatat catctgcgtc atcaggtact gtcgcccaga catcgatctg cacggtaatt
     6061 ctggattcag cctgcccatc aagcacatca gatgccgtgt cagacaccac agaaaacacc
     6121 agccacggcg gagataccgc aggctttccc tccgtcagcg ggaccacata aggataaacc
     6181 tgtcctccgg ccagctgaga caacagggaa tacagtgtgc cctccctcat ttacttaaga
     6241 cctcatcaat agcctgattc attcgctgta tggcaacctg cgccgccagc tcctctgtcg
     6301 tatcgaaagc cgggcgaatg aatggatgcg cgggcatgtt tatcgttccc agctccacaa
     6361 agcgccagta aaatgcattt cggggatcgc tggctttcat gctgttatca ctgtttccgg
     6421 ttcgcaggtt ccgtccgcga atgtggacac ccgagataat ttccccccga cgctttgaac
     6481 gctgagtgag aacaaccaca tttttcttca gtttcccggt tcgctccggc gcacgttcaa
     6541 caactgcatc ccgcataact tcagcaccgg cacgggtggc atcgcgcaga accttattgt
     6601 tttctgccct gctgagcgtc tccagatccc gtgcaatatc cgccaggccg gaaaaatcaa
     6661 gactgaaatc catcacacat tccccttcag gctgcagagt atttcaagcc gggtagcgcg
     6721 tgcatccggt attggtggac cttctatacc cagaatggcc cctttaaatg caccggtcag
     6781 cactttcaga cgtgaagtcg ctgtcacatc gcgccggaat ctcatccaga ctctgaccgt
     6841 agcctgagcg gtttctgctc cgcctgagat tatctccctc ccgctgatac ccttaacttc
     6901 tgcccatacg gtagctccct ccgtcaccgt ctccaccgga tgccctgacg gagagcgggc
     6961 ggtggtgaca ttcagaataa ttacgcgatc acgtaatctg cccgcctgca tgtctcctcc
     7021 tacaaaggaa taaaacgata aggctccagc agagaagaaa aaccaaacgg gactggtgcc
     7081 ttgctgacat ctgaggaatt ttcccggttt tcgtaccagt gcccgaccag caacatgagc
     7141 gccagcaaaa catcatcagc tataagcacc ccttcaggat caccttccgg caccgtctcc
     7201 tcataaagct tacggttgat aaaattttct gccttgcggc aggcagcccg gaaatacagc
     7261 atcagtaact catcatcagt tgcatcatct gtatcaatac ggcactgcgc cctgagtttt
     7321 tccactattg ctgccatcag aaactcctgc ccgcaacact gtgcgggcat aaaaaaaccg
     7381 cgtcggcgcg gtctgtaact gaacaacgag tggttatttg ccagtgagcg ccttgatggc
     7441 tgccacatct tccagcacac agtcaaaacg atggaaagcc agaaatgcca cctgatcaaa
     7501 ctcagcataa cgctcaacca gacgtttcag ttccatataa gtaacgcggc gaatgataaa
     7561 gcggttgaaa tcccccagga aaatgaattt ttttccggta ccaatcccgt caatagcctg
     7621 atcaataaca taagggatcc ccagcacagt agccggcgta ccgcctgcaa tatccggcag
     7681 ccataacggg cgtttctgcc catcctccat ctcttcaata gtctgcaatg tggcatcatt
     7741 gaatgcccag cggtatttcg gcccaccacg atatgccgga tcaatggcat gtttcagggc
     7801 attcatttct ttccaggtga aagcggcaga ggctgcagtc tggatggttc ccgtcaccga
     7861 cgctgccagc ccttttggct gtaacggtga tcccgttccg gtcccctgaa ccagatattt
     7921 tgcctctcca cgaccaatac gctgggcaat acggtttgcc agataagatt caatatccac
     7981 cccactgtcc tggagcagct cattggacac acgaattatt tttgatgaca gctttttagc
     8041 ccccagaata gcggtcccga acgtcacatc ctgttccgtt gcggctgtat tttccgccag
     8101 cagttcgccc tcttcagtcg tgccatcaga cgttgaccag gtgatatcct gcccggttga
     8161 tgtggtcaga agttgcgcca cactggcaat cccgccataa gccttcatgg tgtcaatgat
     8221 tttgttacgc atctgcgtgg gcaccgtata tccgccctga gaatccgttg ttacactctg
     8281 agcccgcagt tcacgcatca gattacgctc ttcagcattc agttctgcaa atccggcacg
     8341 cagaaaacgg ttaaatgccg cagcgcgctt ctcttccacc gcctttttcc cgttctccgc
     8401 ctcattattc tggcgctctt ccggcccgga ctcatccaca tatgcctgat cctgacggcg
     8461 caactcttct tcacgggcga tttgctcatc cagcgcatcc agctcagctt tcgccctgtt
     8521 ccactctgcc cgttgctcat cagtccatgc gttatcacca attttttcat gcagtgcacg
     8581 catatccttt gcaatggtgt ttcgtttttg cttcatctca tgaagtttca tcgtcagtag
     8641 tatccttatg cattaagaag ggtcaaaaga cgctcacgcg ccattcgttc gttaacagct
     8701 ttcttcagcg caccactcgc ccgcgcttcc tgccaggctt tcattgagcg gacaccagag
     8761 tctgcgtcct gataggccgg atatgtcacc gggctgacgt catacagacg agaaatgcgc
     8821 gtgatttccc ggataacaat cccctcgtcg tcttcatacc agctctctcc gtcacgggcg
     8881 acacgaaacg cgaacgagga ctgattaatg tcaccacgca acattggaga cagcaccagg
     8941 tcacaaatag tcggagtatc cggtgcaaca atgtcataac gtaaaccgcg ttcatccacc
     9001 gacaatgaca acgtgccggc agaacttcgt ccgagaatga aattaggatc atgattaaac
     9061 aagccacgta catcatcatt cagtacatca tcaaaagccc ccggcttgat gatttcacga
     9121 aatccccaca gaggttctga acgactgtta aataccgagc cataccccag aatgtgggtc
     9181 ggggcattat catattgttc cgcccgcacc tccccgctgt aacagcgcgt ttcacggtca
     9241 ttcatcgttc ttttcctctt tgcctttcgt atctttaaag ttattcaacg gatttgctgc
     9301 atttacgctg accagcattt catccagacc gtcaaccggg ttcatatcct caaatgccct
     9361 ggcttcattc cgactcatcc agccatctgt aatggcaaag tgatagaact gcgcacgctc
     9421 ctgtggggtc ccacggagca accccgtgag gttgaaacga acgtaatacc cggcagcccg
     9481 ttctgtacgg gtaaacaggc gacggttaag ctcctgctcc cagttcgcaa cccagggcat
     9541 catcgtgtag cgaacaaact gaatcgcctg ctgcgtaata ttcgaaaatg tggctttttc
     9601 caggtcatta atcatgtgcg ccgggacatt aaaaatcccg gcaatcatcg accggttcag
     9661 cttggtcata tcaatgatct gagcatccac cggagaaact gtcagagcgc ggtaatccag
     9721 ttgcgcaggc agcaacatgg ttttattttc ctggctgcga agcgctgtca ccgcccgctg
     9781 ccacatattt ttaagcctgc cccagctttg ttcgttcagt tcatttttca cagaaataat
     9841 accggcagga cgggcattac cgttaaaaaa ggcgctggta tactgctggc cactcattcc
     9901 cataccaatg gtttcagcat gctgcatgat cgggctcagt cccattttct gattgtttcc
     9961 cagcgccctg atatggatca tgtcgtccgg acttaccgca aatgcaccct cttcgttata
    10021 caccccgtaa gtatgacgcc caccggtgtt aagtaacgtg gtttcccatg gcatacagca
    10081 ttcaaggctg gtaacctctc cacgacgatt acgttttacc cacgtataac cattgcccca
    10141 ccccagcaca tgacgctgct tcagttcccg ccacttatag ctggtctgcc aggcattagg
    10201 ttcatcatga acgagccaga acaacggatg atcgcgtgcc ggctgaacat gctcattcgt
    10261 ttttcgcatc acatgcaggg gcatctgagc cacactggat gaaataacat aaatacaggc
    10321 atagacagca gccagcttca tggatgtttc cggactgaca tacacatccc gggcaaaaat
    10381 attatccgtc tcagcggcct ctccggttac cggaaccgag ggattttcca gaggctcact
    10441 gcgaaacaga gcatcaagaa gcatgttttc tcctcatgga caccaccagt gcataaagca
    10501 gcaacaaaca gcccgacagc atcagagacg ctggcagacc tgcatacaga taaacgccag
    10561 cagtgagcaa accgaaaccg atcagcccgg tcatatcagt aataagctgt ttcacagaat
    10621 taacaggtcc tcatcaggat caagcgtgga cagaaagtca ttcacgcccc ctccatttac
    10681 cagaaagcgg ctcatggctg taaaaagcgc aacagggccg tcgattttgg cttccggcgt
    10741 ggatttattc gggaagatgt tgtcgttttt gtccggtttt acagtaacgt tagacatcat
    10801 ccagttcatg accggatgat tgctgtgatg gaaacgcccg gcataaacca gtgattccgt
    10861 ttccttcatg gcctctgaca gattgcggac cgtctgcgga acctccacca gcggtatccc
    10921 ttcttcagcc agtgccaggc tgaactgcat tgcgctccac gggtcaaatc ccagttccct
    10981 gaggttttca ccgccaatcc attccagtaa gtcacttttt atctgagcat gatcgataac
    11041 atcaccatcc gtcaggatga gcttatccat ctccgcccac ttccggtaaa gttctgcctg
    11101 ctgccgcgag catcgttcca gccgtccttc cggaagccag aatttaaaat cagcatgaac
    11161 atgtccgtta tcggttcgcc agagttttgc cgccgcacag atatcaatct tatgagcaag
    11221 gtcgacgccg acccacatgg gatatgtttt cagctcatgt tgtggagcaa tgtattcgca
    11281 cttctcccac ttaatcatat ccatccaggc agattcggca gtgacccaca cattcatgtg
    11341 tttggtaaaa aaattcaccc gcgcagagac ctgctccttc gcttttttcg ccagacgacg
    11401 cagatcatcc cagcgtttac agatgcccag gccaggattc gctttctgcc agaccgtttc
    11461 atcaaacgga tcatctccct catcaagcgt gtaaatgatc gcaaagtaag agtcgtcttt
    11521 tacagcgccc tccacgtcgc tgttatagcc tcgcaatacc ttgatggcgt aatcacgctg
    11581 ctcgtaacaa atcccttcct tgttaaagcc agccgtggtg ataccaaata acagggactg
    11641 cagacgggca ccggttgccg tttccagaac gtcccacacg tcgcgggttt tatgtgcatg
    11701 cagctcatca ataatggcgc agtggatgtt cagaccgtcc aggttgtttg catccgagga
    11761 aagcggttca aattttgatg cgctctgctc ctggtaaatc gccagcttgt tgaaatcaaa
    11821 caaccgcccg agtgtcgacc gggcttttct gaccatattt ttggcgtctt caaacacgat
    11881 tctggcctgg tcacgcgtgg ttgcggctga atacacctca gctccgcctt cactatctgc
    11941 ccccgtcata tacaggccga tacccgatga cagtgttgat tttgcgtttt tacgggcgac
    12001 ttcgttgtac gccgtccgga accggcgcac catcaccgga cgtccgctgc catcgctgcg
    12061 catgacaact tccccggttt cttcattgac cagcggaatg acaaaaccaa aaatattaat
    12121 gaggataaat acatgccagt ccatcaactc aatgggctgg cctgccagtg ccccttttac
    12181 atgaggcaca aatttgtaga aattcaggat gtgctgcgca cggggttcac tgaaataaat
    12241 cccccgcttt tcgccgtact tcagatcatc aagaaaacgc tggcaggcca ggcggacaaa
    12301 ttcgccagca acaatttctc ctgcaacaac acgttcggcg tagcggatcc cgtcagccac
    12361 ttttgccatc agtctctcgc ttttaaaagc tctgccagcg gatcaacatt atccggtccg
    12421 gcggtattta cttttgcccg gcttgccggt gacataccaa attctgcaag catcgcccgg
    12481 atccgcttcc aggcatccgc tttcatcgca gcagccgggt gtgccttaat cagcacatca
    12541 ccgttctgcg tttccgagcg gtaggtgtac ccctcaacat cgagtgtttc gcagtgatgc
    12601 cggtattcgg tataggcttc caccagtagc tcaagcgcac gcgcatcaag ctgagaaatg
    12661 atcccttccg cattcagctc ttccgccatt cgcctgaacc agtacttccc ctgcgcccct
    12721 aaatgttgcg gaattttagg gagacctttt tcatcctttt tagcggtttt tttgaggtct
    12781 ttaactggcc gctttgaggg gttgcctcgt atcaaatgca ggcgtggcgg gattttcggg
    12841 ggtccagaca taatcggttt tacctatcaa tcatttaatc acattccaaa aaaaagtttt
    12901 cgaacctgcg gcgatgcgag gaaaggtcag gcggcggtac tgaacagcca gggttgcaga
    12961 gatttgaccc gcccctcccc tgcagatggg agctgttatc aattgatgcg ttcgcgcgct
    13021 gtttttgctt tatgacaggg ccagcacaga ctctgcaggt tactgtctgc atccgtgcca
    13081 ccatgagctt tcggaatgat gtggtccaca gttctggctt caacggctct cccattgcgc
    13141 aggcagttct gacacagatg attatcacgc ttcagtatgc gcgcacgtat ggcatcccat
    13201 ttcgaaccat agccacgctg gtggcgactc agtcctcgtt gatgctgtac ccatccttcg
    13261 ccacgatgtt tatcgcagta accagaactg tctgtggttg ttcctgcaca tccacgctta
    13321 cggcaggctc gtgggattag tgatggcata aatacctcat accctgcgaa atgtttacca
    13381 cgataaaaag gctacttaat gcactgagtg cggatatact cctgtgcccc ttccagttgc
    13441 atctgcatcg tcatcagccg ctctctgagg gtgaaataat cccgttcagc ggtgtcttcc
    13501 agtcgggggc tggttgcatt atccacgccg gaggtgccgg tggcttcacg cacggtaccg
    13561 gggcaggtgg cgttgatccg caggcgctta cgaccagcgg caacgtcagc gcgaagagtt
    13621 tcattttcag ctctcgcatc ggctaattcc ctcgagtatt ttgcatcgag cgcagcaaca
    13681 tcacgctgac gctgctgcat gtcagcgatg gtggcggtca gctgcttcag ctcactgact
    13741 tttttatcac gctgttcttt gtaggtgatg gcgttatcac ggtaatgatt aaccgcccac
    13801 gacaggcaga cgatgatgca gataaccaga gcataaataa tcgcggcgac tctgctcact
    13861 gctctatccc ccaacaggct aatgcgcttt cctggtcacg acgaataact tgcccatagc
    13921 agttatttga acgtatgcgg caatcgcgcc caccatcctt aatccaccag cgaatcgctt
    13981 cgcatgcccc cttacgatca ccggcattca gccgcttata aaacgtcgac gggaaacact
    14041 taccggggcc aatgttgtac ggacagaatg acgcgatccc cgctttctgg ggttcggtca
    14101 gcggcactct gatgtttttc tccacccacg ccagcgcttt atcacgctca atggcgttaa
    14161 cctgttcgca tttttccttc gacaacttca tgcccggaac gacaggtttg ccatccacca
    14221 tgatggcacc acggcagatg gtccagatac ctgcaccatc acggtatgcc gtggtgtggt
    14281 tgccttcctt ttcatccaga aactggtcga gaatgtcagg cgcaggcgca cctgcggcaa
    14341 tcagcgccag aacggcagcc gacaggccgt atctgatttt tgcgttcatg gatatttatc
    14401 aggatttatc ggttccgaat acctggatat gttaagtctt cagcccagcg gtggagtctt
    14461 cagagaacca gtaattcttc ccggtagctt tcctttgtag gttatccaca cattctgcgc
    14521 ctctaaaatt acggggcgct tttccggcga ctgctcaccc ccttcacata acccggcagc
    14581 aacatccagg aagacctgtc tgatgctcct tctggctgct tcctcataaa actccagcgc
    14641 ggcaccttca acacggtcca gcgagatgtc caggtcaaaa atttcaccgt caaagcgttt
    14701 tttgtcccgt aacgctaaag ttaccgtaac tttattctca aaattgcgga tccctttcac
    14761 aatcagttca tagttttgag tc
//
LOCUS       DAOFSV010000114        14708 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.114, whole genome shotgun sequence.
ACCESSION   DAOFSV010000114 DAOFSV010000000
VERSION     DAOFSV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.114"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            384..617
                     /gene="papI"
                     /locus_tag="QBH27_003504"
     CDS             384..617
                     /gene="papI"
                     /locus_tag="QBH27_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HDQ7015778.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     AGMVQRSPLRRGMATYWFLKGEKQAGQSCSSTT"
     gene            complement(960..1193)
                     /locus_tag="QBH27_003505"
     CDS             complement(960..1193)
                     /locus_tag="QBH27_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ7015779.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(1262..1834)
                     /locus_tag="QBH27_003506"
     CDS             complement(1262..1834)
                     /locus_tag="QBH27_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015780.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKIIGILERALREQSGSRIRRILLR
                     LPCEYDRTFSSRKIWFGMDFIETVSALMNVTSGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGSELDRLILDAWSNVLGFMSPGEAAPYQKQITRDVVLDSRSPDCEEIFQALIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQL"
     gene            complement(2086..2661)
                     /locus_tag="QBH27_003507"
                     /pseudo
     CDS             complement(2086..2661)
                     /locus_tag="QBH27_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3008..4215
                     /locus_tag="QBH27_003508"
                     /pseudo
     CDS             3008..4215
                     /locus_tag="QBH27_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4250..4615)
                     /locus_tag="QBH27_003509"
     CDS             complement(4250..4615)
                     /locus_tag="QBH27_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015781.1"
                     /translation="MIYKISGNQRLIWKFYKTDDNKWRWYCHEKNGYLLSQSDNAYNS
                     QLLCIENAKKQGYSDESVLPLFLHISYIQEKGWKWYQCYGCGYIVKQASVFFPTYQAC
                     IIDAEQECIGILVQLSEKT"
     gene            complement(4658..6022)
                     /gene="pgtP"
                     /locus_tag="QBH27_003510"
     CDS             complement(4658..6022)
                     /gene="pgtP"
                     /locus_tag="QBH27_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter PgtP"
                     /protein_id="HDQ7015782.1"
                     /translation="MLSIFKTGQAADSVPAEKIQVTYRRYRMQALLSVFLGYLAYYIV
                     RNNFTLSTPYLKEQLDLSATQIGVLSSCMLIAYGISKGVMSSLADKASPKVFMACGLV
                     LCAIVNVGLGFSTAFWIFAALVILNGLFQGMGVGPSFITIANWFPRRERGRVGAFWNI
                     SHNIGGGIVAPIVGAAFALLGSEHWQSASYIVPACVAIVFAVIVLILGKGSPHQEGLP
                     SLEEMMPEEKVVLNTRQTVKAPENMSAFQIFCTYVLRNKNAWYVSLVDVFVYMVRFGM
                     ISWLPIYLLTVKHFSKEQMSVAFLFFEWAAIPSTLLAGWLSDKLFKGRRMPLAMICMA
                     LIFICLIGYWKSESLFMVTIFAAIVGCLIYVPQFLASVQTMEIVPSFAVGSAVGLRGF
                     MSYIFGASLGTSLFGIMVDHIGWHGGFYLLGCGIICCIIFCWLSHRGAIELERHRAAY
                     IKEH"
     gene            6421..7638
                     /gene="pgtC"
                     /locus_tag="QBH27_003511"
     CDS             6421..7638
                     /gene="pgtC"
                     /locus_tag="QBH27_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transport regulator PgtC"
                     /protein_id="HDQ7015783.1"
                     /translation="MILLLLLMDVGAVRAQRNELVMATTFSPGAIAWIIQRWQTEPES
                     VMIRTLNRTSASLEQLLDTANVENVDLILTSSPMLLQHLQEHQKLAPFDDAPAESQNL
                     VPESIRATSVAVAISGFGLLINRPALSVKHLPAPADWDDLALPIYQDALLMSSPSRSD
                     TNHLMVESLLQQKGWVKGWETLLTSAGNLVTISSRSFGVADKIKSGLGVAGPVIDNYA
                     NLLLNDPHISFTYFPRSAVSPTYVAILRKSPHADAARRFIHYLLSPKGQRILADANTG
                     KYPVTPLAADNPRATQQQLLMNQPPLNYHLILKRQRLVQRLFDTAISFRFAQLKDAWR
                     ALHSTEARLKKPLPEIRALLTQMPVAAASSEDPVWLAQFDNKSFAEQQMMEWQLWFLN
                     NQRLAIKKLEELK"
     gene            7635..9644
                     /gene="pgtB"
                     /locus_tag="QBH27_003512"
     CDS             7635..9644
                     /gene="pgtB"
                     /locus_tag="QBH27_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PgtB"
                     /protein_id="HDQ7015784.1"
                     /translation="MKNITLWQRLRQVSISTSLRCAFLMGALLTLIVSSVSLYSWHEQ
                     SSQIRYSLDKYFPRIHSAFLIEGNLNLVVDQLNEFLQAPNTTVRLQLRTQIIQHLDTI
                     ERLSRGLSSRERQQLTVILQDSRSLLSELDRALYNMFLLREKVSELSARIDWLHDDFT
                     TELNSLVQDFTWQQGTLLDQIASRQGDTAQYLKRSREVQNEQQQVYTLARIENQIVDD
                     LRDRLNELKSGRDDDIQVETHLRYFENLKKTADENIRMLDDWPGTITLRQTIDELLDM
                     GIVKNKMPDTMREYVAAQKALEDASRTREATQGRFRTLLEAQLGSTHQQMQMFNQRME
                     QIVHVSGGLILVATALALLLAWVFNHYFIRSRLVKRFTLLNQAVVQIGLGGTETTIPV
                     YGNDELGRIAGLLRHTLGQLNVQKQQLEQEITDRKVIEADLRATQDELIQTAKLAVVG
                     QTMTTLAHEINQPLNALSMYLFTARRAIEQTQKEQASMMLGKAEGVISRIDAIIRSLR
                     QFTRRAELETSLHAVDLAQMFSAAWELLAMRHRSLQATLVLPQGTATVSGDEVRTQQV
                     LVNVLANALDVCGQGAVITVNWQMQGKTLNVFIGDNGPGWPEALLPSLLKPFTTSKEV
                     GLGIGLSICVSLMEQMKGELRLASTMTRNACVVLQFRLTDVEDAK"
     gene            9634..10881
                     /gene="pgtA"
                     /locus_tag="QBH27_003513"
     CDS             9634..10881
                     /gene="pgtA"
                     /locus_tag="QBH27_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PgtA"
                     /protein_id="HDQ7015785.1"
                     /translation="MLSSEYSILLIDDDADVLDAYTQLLEQSGYRVFACNNPFEAQAW
                     IQPDWPGIVLSDVCMPGCSGIDLMMLFHQDDQQLPILLITGHGDVPMAVDAVKKGAWD
                     FLQKPVDPGKLLSLVEEALRQRQSIIARRQYCQQTLQVELIGRSEWINQYRRRLQQLS
                     ETDIAVWLYGAPGTGRMTGARYLHQFGRNAQGEFVYRELTPDNAPQLNDFIALAQGGT
                     LVLSHPEHLTREQQYHLVQLQSQEHRPFRLIGIGDTSLVELAASNHIIAELYYCFAMT
                     QIACLPLTQRPDDIEPLFRHYLCKACQRLNHPVPEVGKEMLKEMMRRMWPNNVRELAN
                     AAELFTVGVLPLAETANPLMHVGTPTPLDRRVEDAERQIITEALNIHQGRINEVAEYL
                     QIPRKKLYLRMKKYGLSKEHYKI"
     gene            11128..11241
                     /locus_tag="QBH27_003514"
                     /pseudo
     CDS             11128..11241
                     /locus_tag="QBH27_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11474..11797)
                     /locus_tag="QBH27_003515"
     CDS             complement(11474..11797)
                     /locus_tag="QBH27_003515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015786.1"
                     /translation="MSNRYWMISFHKNTRNLTVRSVPKKDIAHNIKLIIRPLNISIEK
                     FLSIEYTNVEGIPLTSRRITIMGLKPGPKPIAKSVGKPDQRRRDNKDTPGNTPGLKPS
                     KSTGK"
     gene            complement(11862..12506)
                     /locus_tag="QBH27_003516"
     CDS             complement(11862..12506)
                     /locus_tag="QBH27_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015787.1"
                     /translation="MMKKDMADSVSMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPSFRSEKEAESWLNELSFA
                     VIAPLYRVVPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQRQRITKILNESDTRRVAEEIKFRIQYLQDEEQKRVLSDLVNVLISKR"
     gene            complement(12527..12796)
                     /locus_tag="QBH27_003517"
     CDS             complement(12527..12796)
                     /locus_tag="QBH27_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDQ7015788.1"
                     /translation="MVAQKLEAAGCWRRASARWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIASPSGEIFRKGTPVC"
     gene            complement(12875..13520)
                     /locus_tag="QBH27_003518"
                     /pseudo
     CDS             complement(12875..13520)
                     /locus_tag="QBH27_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
     gene            complement(13505..>14708)
                     /locus_tag="QBH27_003519"
     CDS             complement(13505..>14708)
                     /locus_tag="QBH27_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ7015789.1"
                     /translation="LTGENVLRAAIARIEWIFETFPSVCLSFSGGKDSTVLFHLLADV
                     ARRKKRRFSVLFIDWEAQYQCTIEHIQKMREMYHDVTETFYWVALPLTTVNGVSQFQP
                     EWICWEPGVTWVRQPPEEAITDMTYFPFYRYAMTFEEFVPAFSSWFADNKCGVAVLTG
                     VRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKTRDIWIYHTRTR
                     AIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWARMCERVSGAASG
                     ALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKIAVYLRWYQTR
                     GFPDDIPDEQENDLGCRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKE
                     RRNEWGIL"
BASE COUNT         4060 a         3488 c         3432 g         3728 t
ORIGIN      
        1 gcccggaaat actcttcatg caataagtta aatttagttt tttatgttgt aaatattgat
       61 ttaaacgatc ttttaaccca caaaacaaaa ttaaactaga ataaaacact aataataaga
      121 atgcactcag ataaaaacat catagtaaat taaatttaca gcataaaaga tcgtctaaat
      181 gcatttttac ggactttctt ttcacagaaa aataaaacgc aaaatgtgat acatatcaaa
      241 taaagccaat aaaaagcaaa ttcactaaat tgaacaaata aagcaaacac agcaaacaca
      301 gcaaacacag agaataaaaa tacctttccg ttgataacag aaagcatgat ttttattatt
      361 cgaagaaact ttgttacagt gagatgagtg aatatatgaa gaatgaaata ctggaatttc
      421 tgaacaggca taatggaggg aaaacggcag aaattgcaga ggcgctggcg gtaacggatt
      481 accaggcccg ctattacctg ttattactgg aaaaagcagg tatggttcag cgctcaccat
      541 taagacgggg catggcaaca tactggtttc tgaaaggaga gaagcaggcg gggcagagct
      601 gttcttccac aacttaataa ctcaatctgt gggcatgctt cactgaaaca gataaatgtc
      661 agcgccagtg atataaggcg taacgactaa caatcttggc gttgacagat gtttcttttc
      721 taatttactg aaccggcgca gacttacaga aaaacactcc ggcaccggtg taatgccagt
      781 tataatcaat aagaatagaa tgacgaatca gtcaggaata gctataacct gcaggctgac
      841 tggagaatgt ctgcaagaag atgaacgtaa atgtcgccag caccaggggc agggccgggt
      901 tccacaatac cagcgctggc attttgcaca ttgccagcgt actggcatta caacacgttt
      961 catgctgctg ccccccggga ttcctcgatt cgttgctgca tccactgctc cacctcactt
     1021 ttgtaccagc ggctgctgcg ccccagtttg atgggtttag ggaaatgacc ttcactgatc
     1081 agcttgtaaa accatttatc tgtcatgcca gtgaaactgg tgatgtagtt catgtcgatc
     1141 aggggatcgt cggcacgcag caatacgctg tcgtggcttg ttgtggtcaa catggtcaga
     1201 atgtcctctg tgagatttat gaaggttggg caaatgcggg tgaatctact gaacggagtt
     1261 ttcagagctg gcctgctacc ggataccagg gattaccggc aaggtaacgg gcttccggat
     1321 cgcgtacacc acgacgatcg cgggcgaaat cactgaacgc ccagatcagc gcttgaaata
     1381 tctcttcgca gtccggtgaa cgactgtcca gcaccacatc ccgggtgatc tgcttctggt
     1441 acggtgccgc ttcacccggt gacatgaagc ccagtacgtt gctccaggca tcaagaatca
     1501 gccgatccag ttcagagcca tcgaaatact cctgtctgac gcacagcacc acgtggtatt
     1561 ccggcttttc aggatgacga gtcaggagcc agcagagatc acgtccggag gttacattca
     1621 tcagcgcact gacggtttcg ataaagtcca taccgaacca gatctttctg ctgctgaagg
     1681 tacggtcata ttcacatggc agacgaagca ggatccgtcg tatccgggag ccggactgct
     1741 cacgcagcgc acgctcaagg ataccgatga ttttgttctg ataagacggc cagaagggtg
     1801 ccaggctata gtcgaaataa ggaatagcat tcataagata tgttccgtta tgcgcagccc
     1861 acagtgcgtc ctgcccgtga atgagctgac gtgttatggc ttgcggatga gattgatgga
     1921 ataagtgaag aactgaagca gaacgggaag atccggtaac cggcaaggaa cagaacaccg
     1981 gattcaggtt caggatgaat gatctccgtt gacttcagtt cacatcatta atcaatgcgg
     2041 taagttttta ccgacggagt gtttccagga gcggaggctc aggccttaag cttcagaagt
     2101 gaggcttacg aggacgccct gcagcaggac gttcaggaac ttcattgcag ccgtaggcac
     2161 acagcccgtc tttttgttct tctttcgcca gataatgcag ggcaccgcga atattatcga
     2221 tgctttcagg atcgttgtgg cgcacaggaa tgttgatgtt gtatgcataa tgcggctgat
     2281 atgtacagcg gtgcgccgaa ccacagttat acgtaattgc ctgccagcat tccgctatcc
     2341 gctccgcaaa gggatatata tttttttaac cctctgacga tcgatccaga aaacgacatg
     2401 cgcatgaaaa ccatgatcag ccgtccattc aatcacccag aagaaaccaa cgatattttc
     2461 aaattgttct acctgctcca gcagcatacg caactgcaat tcaagccagc gatgatcagg
     2521 ttgtaagaaa tcaggcgtat ctttcctgta gaagaaatcc atgcgtaagg cccgcaggca
     2581 ggaatagcgt gccatcattt gattgatgtg attgtcaagt aaagcggtaa aaaaccagtt
     2641 tggtgtgtaa gatttaggca tgttgatgac tcctgaaagt taatgatgtt ttaagctctg
     2701 tgagcgaatt aatgctttcg ctcataatca gatctaaaac ggtaataact tacagatggt
     2761 atttatcagg catgctgtaa cagaatggat acgacggatt attaacgaca cacagcgttg
     2821 tgctgaaggg tatgagttat ctgatggcat tgtctataga gatgagataa acgaggtgcg
     2881 aagaaaatac agattcaata caacacagtg aaatactaga ctggccccct gaaataccag
     2941 acagtagatg tatcttaaaa taagaggtag gcttggatat atgtctaaca ctagttctaa
     3001 ttttgagatt gctggcgttt tgttaggaaa agaggtgcgt aaacgtaaaa cacctcagaa
     3061 aaaaaatcgc aattattcaa cagactatgg agcctggcat gactgtgtcc cacgtggcac
     3121 gtttgcatgg cattcagccc agcctgttgt ttaagtggaa aaaacaatac ctggaaggaa
     3181 gcctcactgc tgttgctgcc ggtgaagacg ttgttcctgc ttctgaactg gccgctgcga
     3241 taaagcagat taatcagctt cagcgtttac tgggcaagaa aacaatggaa gttgagatcc
     3301 tcaaagaagc ggtggagtac gcacaatcgc gaaaatggat agcgcatgtg cccttgttac
     3361 cttcggacaa ggaatagctc cggtaagccg tgttctgggc gtgtcgcgtg cgcagctaac
     3421 attacggatg aaggcatctg ataacaagcc agataaacgg cgtcagagac gcgatgaggc
     3481 tgcggatgca gaagtcttat cgcgcatcct ggatatcatt ggcgatatgc cagcatatgg
     3541 gtatcgccgt gtctgggcga tactcagacg gcagtcccga aatgaaggct tgccgttcgt
     3601 gaatgcaaag cgcgtttacc gaataatgag cgaaaacagc ctgttgttgt tacatgacaa
     3661 accatcacgg ctgcagcggg aacataaagg ccgaatatcg gtgaaggaaa gcgaccagcg
     3721 ttggtgttct gatggcttcg agttcggttg tgatgatggc gaaaaagtcc gggttacgtt
     3781 cgccctggac tgttgtgatc gtgaagccat cgactgggca gccagtacgg gaggttacga
     3841 taaagcgaca gtacaggatg tgatgtcagg agcgatagaa aaaggctttg gcgataaagt
     3901 gccggaagag cccatccagt ggctgacaga taacggttca gcgtacagag cgcatgaaac
     3961 acggcagttc gccagagagc tgaatctgga gccctgcacg acggcagtca gcagtcctca
     4021 gagtaatgga atggcagacg gttcgtgaaa acgatgaagg aagactacat cgcgttcatg
     4081 ccgaaaccga atgtaagaac ggcagtgcat aaccttgcag tagcgatcga gcattacaat
     4141 gaaaaccatc cgcacagtgc gttgggttat cgctctccgc gagaatatcg acgtcagcgg
     4201 gtaacgttaa cttaagatac atccactgtc tggcatttca aggggtggct caagtttttt
     4261 cagacagttg aactagtata ccaatacatt cttgttcagc atcaatgata catgcttggt
     4321 atgtcggaaa gaaaacagat gcttgtttta cgatatatcc acagccataa cattgatacc
     4381 atttccagcc tttttcctga atgtaggaaa tatgtagaaa aagtggcaag actgattcgt
     4441 ctgagtaccc ctgtttttta gcattttcaa tgcataacaa ttgcgaatta tatgcgttat
     4501 ctgattgaga caaaagatat ccatttttct catgacaata ccatctccat ttattgtcat
     4561 cagttttata aaatttccat attaatctct gattgccaga tatcttataa atcataatat
     4621 gctccactcc ccagggaaac gaccctgggg aaggtaatta gtgctctttt atatatgcgg
     4681 ctctgtgacg ttcaagttca attgcaccac gatgtgataa ccagcaaaaa atgatgcaac
     4741 aaataatacc gcagccaaga agataaaatc cgccatgcca gccaatatga tcgaccataa
     4801 taccaaacag gctggtgccc agagatgcac cgaagatata gctcataaaa ccacgtaaac
     4861 ctaccgcgga gccaacagca aagctgggca cgatctccat agtctgaacg gaagccagaa
     4921 attgtggaac gtaaatcaga caaccaacaa tggcagcaaa aattgtcacc ataaacaacg
     4981 attcactttt ccagtagcca atcaggcaaa tgaaaatcag cgccatacaa atcatcgcca
     5041 atggcatacg acgcccttta aacagtttgt ctgacaacca accggcaagt agcgtggaag
     5101 ggatcgcggc ccattcaaaa aataaaaatg cgacgctcat ttgttctttt gaaaaatgtt
     5161 tcaccgtcag cagataaata ggcaaccagc taatcatccc gaagcgcacc atgtatacaa
     5221 atacgtcaac cagtgagaca taccaggcat ttttattgcg taatacataa gtgcagaaaa
     5281 tctgaaaggc gctcatgttt tctggtgctt ttaccgtctg tcgggtattc aggacgactt
     5341 tttcttccgg catcatctct tccagagagg gtagaccttc ctgatgtggg gaacctttac
     5401 cgagaatcag cacaattacc gcaaaaacga tggccacgca ggccggaacg atataactcg
     5461 cactttgcca gtgctcgctg ccgagtaggg caaaagcggc accaacaatc ggtgcaacaa
     5521 taccaccacc gatgttatga gagatattcc agaaagcacc aacccgacca cgctcccggc
     5581 gagggaacca gttagcaata gtgatgaaag aaggaccaac gcccattccc tggaaaagac
     5641 cattcagaat aaccaatgcc gcaaaaatcc agaatgcggt gctgaatccc aggccaacgt
     5701 taacgatggc acataacacc agcccacacg ccataaagac tttcggactg gctttatcgg
     5761 caaggctact catcactcct ttgctgatac cataggcgat aagcatacag ctactcagta
     5821 cgccaatttg tgtggcgctg agatctaatt gctctttaag ataaggcgtt gataaggtga
     5881 aattattacg cacgatatag tatgcaagat accccagaaa tacgctaagt aacgcctgca
     5941 tacgatagcg acgatatgtc acctgaattt tctccgccgg aacactatcc gccgcttgcc
     6001 ccgttttaaa tattgataac atgtcttcac ctgtatttta tattgatatg aacatcagga
     6061 gtattctgac aatgagagtt atataattga gatgaaatat caaactgaaa aaatgtgtca
     6121 caattgacac aaaataataa aatcaaagcc tgtttttgac ccattttatt gatgcataga
     6181 cagggcagat ttatgcttta tctggtttgt tgttagcatg tgcggatatt aacaaatctc
     6241 atgctgatta tcgggtaatc tctgattaat atattaacgg tctctttgaa cagacgtgtt
     6301 atcagacgaa gtgtctgaaa cgggtaattt ttgtctcaat gcgatttttg cggtgtatta
     6361 atatttatga cctggatcac aaaccgattc tttagttggt gcgaaatcgg catcaaaacc
     6421 atgattcttt tactgttgtt gatggatgtc ggggccgtca gggctcaacg aaatgaactg
     6481 gtgatggcca ccacattctc acccggagct atcgcgtgga taatacagcg ctggcaaaca
     6541 gagcctgagt cggtaatgat ccgtacgctt aaccgcacca gtgcctcact ggaacagttg
     6601 cttgatacgg ccaacgtaga aaacgtcgat ctgatcctga cttcatcacc aatgctgctc
     6661 cagcaccttc aggagcacca gaaactggcc ccgtttgatg atgcacccgc agaaagccaa
     6721 aacctggtgc cggagtcgat ccgtgcaacc tccgttgcag tagcaatatc aggttttggt
     6781 ctgcttatta atcgtcctgc gctttctgta aaacaccttc ctgcccctgc tgactgggac
     6841 gatcttgctt tgccgatcta tcaggacgct ttattgatga gtagtccgtc gcgttcagat
     6901 actaaccatt taatggttga gtcattacta cagcaaaaag gctgggtgaa aggatgggaa
     6961 acgttgctga caagtgcagg aaacctggtg acaatctcct cccgcagttt tggcgtagcc
     7021 gataaaataa aaagcggact cggcgttgcc ggccccgtta tcgataacta tgctaattta
     7081 ctgttaaatg acccccatat ctcttttacc tatttccccc ggtctgcggt atcccccacc
     7141 tacgttgcaa ttctcaggaa aagcccacac gccgatgcag cccgtcgctt tattcactac
     7201 ctgttaagtc cgaaaggaca gcgtattctg gccgatgcca atacaggaaa atatccggtg
     7261 acaccacttg ccgcagacaa tccgcgggca acgcaacaac agctcctgat gaatcaaccc
     7321 ccgctgaatt atcaccttat cctgaaacgt cagcgtctgg tgcagcgctt gtttgatacg
     7381 gcaattagct ttcggttcgc acagctaaaa gatgcctggc gagcactgca cagtaccgag
     7441 gcacgcctga agaaaccatt accagaaata cgtgcactgt taacacaaat gccagttgca
     7501 gcggccagta gtgaagatcc cgtctggctg gcacagttcg ataacaaaag ctttgccgag
     7561 cagcaaatga tggaatggca actctggttt ctgaataacc agcgcctggc gataaaaaaa
     7621 ctggaagagc tgaaatgaaa aacataacgc tgtggcagcg tttaagacag gtcagtatca
     7681 gtaccagctt acgttgcgca tttctgatgg gggcacttct gaccctgatt gtcagtagtg
     7741 tcagtctgta ttcatggcat gaacaaagct cacaaattcg ttactcgctg gataagtatt
     7801 ttccccgtat tcactctgct ttccttattg aagggaacct gaatctggtg gtagaccagc
     7861 taaatgaatt tttgcaggct cccaacacca cggtgcgatt gcaacttcgt acccagatta
     7921 ttcagcatct cgacaccata gaacggctta gtaggggact gtcatcccgg gaacgccaac
     7981 aactgacggt cattttgcag gacagtcgat cactgttatc cgagttggat cgtgcgcttt
     8041 acaacatgtt tttactacgg gaaaaggtga gtgagctatc agcgcggatt gactggttac
     8101 acgatgattt tactaccgag cttaattctt tagtgcagga tttcacctgg cagcagggaa
     8161 cgctgctgga tcaaatcgcc tcccgacagg gcgatacggc gcaatacctg aagcgatctc
     8221 gtgaagtgca aaatgaacag cagcaggttt ataccctggc acgcattgaa aatcagattg
     8281 ttgacgatct gcgtgacaga ctcaatgagc tcaaatcagg acgtgatgac gacatacagg
     8341 tggaaactca tctccgttat tttgaaaatc tgaaaaaaac ggcagatgaa aatatacgta
     8401 tgctggatga ctggcctggc accattaccc tgaggcagac catcgatgaa ttgctggata
     8461 tgggaatcgt aaaaaacaaa atgccggata cgatgcgtga atatgtcgcc gcccaaaaag
     8521 ccttagagga tgccagtcgc accagggaag cgacacaggg tcgcttcaga acgttactgg
     8581 aagcgcagct tggcagtact catcaacaaa tgcagatgtt taatcaacga atggaacaaa
     8641 ttgttcacgt tagcggtggg ctgatcctgg tggcgacagc actggcgtta ctgcttgcat
     8701 gggtattcaa ccattatttt atccgctcac ggttggtgaa acgctttacc ctactgaatc
     8761 aggccgttgt gcaaattggt ctgggaggca cggaaacgac tattccagtt tatgggaatg
     8821 atgaactggg gagaattgca ggattattac gccatactct cggccaactc aatgtgcaaa
     8881 aacagcaact tgaacaagaa attaccgatc gtaaggtgat agaagccgat ctgcgtgcca
     8941 cccaggacga actgattcag acagcaaagt tggcggtagt cgggcaaacg atgaccacgc
     9001 tggcccacga gatcaatcag ccgctaaatg cgctgtcaat gtatctgttt acagcccgca
     9061 gggccattga acagacccag aaagaacagg ccagcatgat gcttggtaaa gccgaagggg
     9121 tgattagtcg tattgacgcc attatccgtt cactacggca gtttacccgg cgcgccgaac
     9181 tggaaacatc actccatgcc gttgatttag cacagatgtt cagtgcggcc tgggaacttc
     9241 tggccatgcg tcatcgctct ctgcaagcta cgcttgttct gccgcaaggt acagccacag
     9301 tttcaggtga tgaggtcaga acccagcagg tactggttaa cgtactggcg aatgcgcttg
     9361 atgtttgtgg gcaaggcgct gtcattaccg ttaactggca aatgcagggt aaaacgctga
     9421 acgtattcat tggcgataat ggcccgggct ggcctgaggc attgttgcct tcgttattga
     9481 agccgtttac caccagtaaa gaagtaggac tgggtattgg tctttcaatt tgtgtgtcgt
     9541 tgatggagca aatgaaaggg gaattgcggc tggcatcaac gatgaccagg aatgcctgtg
     9601 tggtactgca attcagacta acggatgtgg aagatgctaa gtagtgaata ttcgattctg
     9661 ctgattgatg atgatgctga cgtgctggac gcctacacac agttacttga acagtcgggc
     9721 tatcgtgttt ttgcctgtaa caatcctttt gaggcgcagg cgtggataca accagactgg
     9781 ccaggcattg tgctaagtga tgtgtgtatg cctggctgct ctggtatcga tttgatgatg
     9841 ttattccacc aggatgatca acagctcccc attctgctta tcaccgggca cggcgatgtg
     9901 ccaatggcag ttgatgcggt gaaaaaaggc gcgtgggatt ttttgcaaaa accggtcgat
     9961 cccggcaaat tactttctct ggttgaagag gcactacgcc agcgtcaatc aatcattgcg
    10021 cgtcgtcagt attgccaaca gacattgcag gtggagttga tagggagaag tgaatggata
    10081 aatcagtatc ggcgacgttt acagcagttg tctgagacag atattgccgt ctggctctac
    10141 ggtgcccctg gcacaggacg gatgaccggt gcacgttatc tgcaccaatt tggacgtaat
    10201 gcacaaggcg aatttgtcta ccgcgagctg acaccagaca atgcaccaca gcttaatgat
    10261 ttcattgcgc tggctcaggg gggaacatta gtgctcagcc atcctgaaca cctgacgcgt
    10321 gaacagcagt accatctggt tcagttacaa agccaggaac acagaccttt tcgcctgatt
    10381 ggtattggag acacctcgct ggtcgagctg gccgccagca accatatcat cgcggagctc
    10441 tattactgct ttgcaatgac acaaatcgct tgcctgccat taactcaacg ccccgacgat
    10501 attgaaccac tgtttcgcca ttacctttgt aaagcatgtc aacggcttaa ccatcctgtt
    10561 ccggaagtgg gaaaagagat gctcaaagag atgatgcgcc gcatgtggcc aaataatgtg
    10621 cgagagctgg ccaatgcagc agaactattt actgtaggag tattaccgct ggcagaaaca
    10681 gcaaatccgt tgatgcacgt cggaaccccg acaccgcttg acagacgagt ggaggacgct
    10741 gagcgacaga taatcacaga ggcgcttaat atccatcagg ggcgtatcaa tgaggttgct
    10801 gaatatttac aaatcccacg gaaaaaactc tatctcagaa tgaaaaaata cggtcttagt
    10861 aaggagcatt ataaaattta accatcagac ttgtgcgctt gttcataatg ttatgtcatt
    10921 gcctgatgtt gataaaaaga cggacgctcc ccctgtaaga cctgtctctt atacacaagt
    10981 ccccacccgg ggatttgtga tgtctgtcag tctgctttag ggggggctct ttccatccgg
    11041 tactttcctt cctccccggt agccattttt tgaacttttt cttcacccgt tgatcttctc
    11101 ttatatttca ttttattcga ctctgttatg gtactttctg attgttattc atgggctaac
    11161 gagcagtttg gacatgcccg tctcgatgat ccgcgtagta cacgacgcct tgtatcactg
    11221 gcctcttctc tcgctcatgc caggtcgtga tcgattcaga tgccattttg caccttcaga
    11281 atttgagctt gctgcagttg ttcctgataa ggatctggat aaatatgacg agtatttacc
    11341 agagtctttg cttgcgaaag ggtacggggc aaaggcgtat gatatagaaa gaacgagcat
    11401 ctggttgctg aaacaccttc agtggactgg atatatagtc agtgtataaa tgcttgttta
    11461 tacccttatt tttttactta cctgtggact tacttggctt tagtcccgga gtattgccag
    11521 gagtatcttt attatcgcgt cggcgttgat ccggttttcc taccgactta gcaattggtt
    11581 ttggacctgg tttaagaccc atgatagtta tccttcgaga tgtaagagga atgccctcaa
    11641 cattggtata ttctatactg agaaattttt caatggaaat gttcaacggc cgaatgatta
    11701 acttgatatt gtgagcaata tctttttttg gcacagagcg aaccgttaga ttgcgcgtat
    11761 ttttatggaa ggagatcatc caataacggt tactcaccga tcttcttcat ggtaatacgc
    11821 acctgtttta ttaacatata gaaaaattac tccggcatgt tttatctttt tgaaatcagg
    11881 acatttacca gatcacttaa aactctcttt tgttcttcat cctgaagata ttgaatccgg
    11941 aatttaatct cttctgccac ccgtcttgta tcggactcat taagtatttt tgttattcgc
    12001 tgtctctgat gttcaggctg gtcttccata tttcgtaaaa ccagatgaaa taatatatca
    12061 taaagtgcgc aatgccatga ataaccatca ttggttaaaa taagctccca aacccgctcc
    12121 tgctcggcaa taagctgcag aacaaaggac tcaacctgtt ctgtttgttc ggggacaaca
    12181 cggtatagcg gagcaatcac agcaaaagac agttcattta accaggattc cgcttccttc
    12241 tcgctcctga acgatggaca gttttgtatt cttcgttcgt attcacgggt aatctgctcc
    12301 agaatatcct ccggcggtgt cagccactta ttcctgagca atacactggt tgatggatga
    12361 cagctggcta tttcagcaat aatttcacgt aatgtctccg ggctgaactt cttcagctga
    12421 ttgtagtgct cccgggtaaa accatgttca tcgcataaaa gttgccatgt cattgacaca
    12481 ctgtccgcca tatctttctt catcattaat acctgttacc tgaatatcaa cagaccggtg
    12541 taccttttct gaatatctcc ccggacgggc tggcaatccc cattcgcctc agcgtggcat
    12601 ctgcagcttt cgccacttcg ctgatatcca gcttttcagg tagcggcggg gacgatatct
    12661 gcgccagaca ataattccgg cgcaacaaca accactcccg ttgtgcttct gtacattcaa
    12721 catttcccat aacaaaaagc cagcgggcag aagctctgcg ccagcaaccc gccgcctcca
    12781 gcttctgtgc aaccatgtct tccacgactt ccggggtact atttttcctg ctcaaaacat
    12841 caactcactg aaataatact catatatgac ggtctcagcg aaccgtccat gccgggctga
    12901 aatcctcttc ctgaaacaac tccgtcagtc cgctaatctg tttcagcctc agcacttcat
    12961 cctggtccat ccccaggctc tgtaccaatg cgttcatccg tccagcccag gcgtgacagg
    13021 tccctgacaa tatcagacat tgaggttatc tggtgtttac ccctggcccg gttatgccgt
    13081 atggtggccg ccatacgtgt ggcctgtccc tttctttccg gattaataca ggccactggc
    13141 aaccagcctt tcaggcgttt tcctgtacct gcttccctgc ccagtaactg acgatgaaaa
    13201 ccatccacca ccagataggg gttcttctcc gccgacacca ccacgggttg ggtgaaacca
    13261 tctttctcca gtgactgctt cagcagtttc ttctcacccg gcgccatgac attggggtta
    13321 taatcgttgg ccaccacctc atctgctttc acccacagca cacagtccac aggctcctgt
    13381 gcaaaagggc tgacttcatg aagtaccctg cgcagatgat tcagagcctg tatagtctct
    13441 ttttctgatc gttcctggct gaagtactga cgaatcatgt cactcagtac ctccacttca
    13501 ggtgtcacag aatcccccat tcattcctcc tttccttcat acgctgcagg taacgttcat
    13561 agtgccgggg tttgttcggg ctgaaggaca gcgtccggca ccagaaatca ttctttataa
    13621 gagtcttaca gatacgtcgc caggacggga tatcccggca gcccaggtca ttctcctgtt
    13681 catcgggaat gtcatccggg aagccccgcg tctgatacca gcgcagatag acagctatct
    13741 tgttgcggta atgttctgct gttctttccg gcatcacatc cagcagaaac atcgcgtagc
    13801 tgcgccaggt atgatgggct ggttttgata tgcgcttgcg cagggcaaaa taagcaccgc
    13861 tttcattggc ataaagtgcc ccgctggcag cccctgatac ccgctcacac atcctggccc
    13921 aggtatccgg ctccagaaca tggtaaagcc acagtccctt acgttgttca ggaccaaaag
    13981 gttcacagac acgcatgttg cgtaacggta cgccggcacg gtacatcagg tcatacaggg
    14041 gattgtagat ggcacgggtt ctggtgtgat atatccagat atcacgagtt ttccagtcat
    14101 acagtggata catggtgtag taaaaccctt caggcgatgc cgtggtccag ggcttatcat
    14161 cggcgtatct cagtttgcgc tgggacacca gtcccatgaa ccggttaagg gactcatccg
    14221 cacggacacc ggtcagtact gccacaccac atttattgtc tgcaaaccag gaagaaaatg
    14281 ccggaacaaa ttcttcaaac gtcatggcat accgataaaa tgggaaatac gtcatgtctg
    14341 taatggcctc ttccggtggc tggcgaaccc aggtcactcc tggctcccag catatccact
    14401 ccggctgaaa ctgcgagacg ccgtttaccg tggtcagggg aagtgccacc cagtaaaagg
    14461 tttccgtcac atcatggtac atttcccgca tcttctgaat atgttcaatg gtgcactggt
    14521 actgagcctc ccagtcaata aacagtacag agaaacgacg ttttttcctg cgggccacat
    14581 cagccaaaag atggaaaagc acggtggaat ctttaccccc ggaaaaagag agacaaactg
    14641 atggaaatgt ttcaaaaatc cactcaatac gagcaattgc tgcacgcaga acattctctc
    14701 cagtaagg
//
LOCUS       DAOFSV010000115        14660 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.115, whole genome shotgun sequence.
ACCESSION   DAOFSV010000115 DAOFSV010000000
VERSION     DAOFSV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.115"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1465)
                     /gene="dtpD"
                     /locus_tag="QBH27_003520"
     CDS             complement(<1..1465)
                     /gene="dtpD"
                     /locus_tag="QBH27_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDQ7015790.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRA"
     gene            1736..2479
                     /gene="ybgI"
                     /locus_tag="QBH27_003521"
     CDS             1736..2479
                     /gene="ybgI"
                     /locus_tag="QBH27_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDQ7015791.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKEMVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            2502..3158
                     /gene="pxpB"
                     /locus_tag="QBH27_003522"
     CDS             2502..3158
                     /gene="pxpB"
                     /locus_tag="QBH27_003522"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDQ7015792.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPACDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            3152..4084
                     /gene="pxpC"
                     /locus_tag="QBH27_003523"
     CDS             3152..4084
                     /gene="pxpC"
                     /locus_tag="QBH27_003523"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDQ7015793.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            4074..4808
                     /gene="pxpA"
                     /locus_tag="QBH27_003524"
     CDS             4074..4808
                     /gene="pxpA"
                     /locus_tag="QBH27_003524"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDQ7015794.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETIFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            4844..5635
                     /gene="nei"
                     /locus_tag="QBH27_003525"
     CDS             4844..5635
                     /gene="nei"
                     /locus_tag="QBH27_003525"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDQ7015795.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAPQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(5632..6678)
                     /locus_tag="QBH27_003526"
     CDS             complement(5632..6678)
                     /locus_tag="QBH27_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDQ7015796.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMHG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIIAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6858..7571)
                     /locus_tag="QBH27_003527"
     CDS             complement(6858..7571)
                     /locus_tag="QBH27_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDQ7015797.1"
                     /translation="MSESALLTSVTLSSQVKNHILNLIAEQKLTPGMPVPSEVQLIQK
                     LGVSRGIVREAYRSLAALGILETHSGKVPRIKKFDDEVLRVVFGFALATEQVTTEQIL
                     QVRRWLELGVVEAAARNGEEEDFANLRAEMAKIREQFNDVESFITHDIRFHQLLAVAS
                     KNPLFMMLLQALHQQLRQSIQEGLNAQSDQKEYGNHIITLHQKICDAVCARDVETATQ
                     AMKSHFDSPVSALINNSSS"
     gene            7835..8521
                     /locus_tag="QBH27_003528"
     CDS             7835..8521
                     /locus_tag="QBH27_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HDQ7015798.1"
                     /translation="MWKSDNELFALMKTRLFTAVVGDILDTLGYMHQFVSPAIKPLDV
                     NTVVAGRAMPVLESDVYETKSTSGKTAIGEKPFGIMFEALDALKENDVYITTGSSLRY
                     ALWGGLMSTRAMHLKAAGAVLDGYARDSAEILELKFPTFCHGTYAQDQGPRGKVIDFG
                     VPIEFHGVRVNPGDIVFGDRDGLLIIPQVVEKEVITQALEKVATESEVRKAISDGMST
                     VQAFETFGVM"
     gene            8534..9547
                     /locus_tag="QBH27_003529"
     CDS             8534..9547
                     /locus_tag="QBH27_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDQ7015799.1"
                     /translation="MKALTIEQGSQARFVDIPEPVCGKGEVLINVERVGLCGSDLNTW
                     RGLNPLVNYPCIPGHEIGGTVLATGDMAGAIKPGDRVVVLPCTECGVCSSCLSGRPNA
                     CKNNQTLGVQRQGGMAQQLTMPAHKVIVCNALSVDELALVEPLAVGLHAARRAQINVG
                     EWVAVIGCGVIGLGAIAAASAMGARVIAIDIDDRKRNIALECGAQRFLNSQQCDVAAE
                     LVLLNDGRGPEVVIEAVGNQQTIEQATGLVAFSGRVVYVGYAKQPVSYQSAQFLMKEL
                     DIRGSRNATVADFRAVLALMESGRYPVSNLVSAHYPLEQADHAFSDWASDPGAYTKIL
                     IDL"
     gene            9559..10314
                     /locus_tag="QBH27_003530"
     CDS             9559..10314
                     /locus_tag="QBH27_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7015800.1"
                     /translation="MNAFSLHNKTALITGAASGLGLAMAKCMIVSGAKVIIADLNGEL
                     AQATAGTLGSQASWIQMDVGDTAAAQEKVDVLISEHGAIDILVNNAGNHCKKPIEEMS
                     VSDFESVLDVHVVGAFALTKALVPHMKQRGKGCVLFTASMTSFLGQPLVTGYSAAKSA
                     YLGMIRGMTTELAVHGIRVNGIAPGWIDTPMLRKAIEGDDERRNKILGRTPMKKFGKP
                     EDIGWAAVYLASDAAAFVSGQVLVVDGGALIGF"
     gene            10329..10640
                     /locus_tag="QBH27_003531"
     CDS             10329..10640
                     /locus_tag="QBH27_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDQ7015801.1"
                     /translation="MRKRALKLHAKDNCVVALNDIASGDIVWWDEGEMVACSSVTLGH
                     KLACEALNNGDSILKYGAIIGHATRDIAMGEHVHSHNLASNAIAIFHHDDAGYQSEEK
                     A"
     gene            10640..11836
                     /locus_tag="QBH27_003532"
     CDS             10640..11836
                     /locus_tag="QBH27_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDQ7015802.1"
                     /translation="MEGYVRQNGRPGIRNTVQVIFMVECASHVAKKIAEGFPCHDVQY
                     FGFSGCYPSDYGYRLVKNLATHSNVGGVLIISLGCENFDRRRLEQEVRESGRPCHTLV
                     IQENKGTTNTITLGKQLVAEMLEQLADTPRCILNWSDLVVGTICGGSDGTSGITGNPA
                     VGRAFDQLLEKGATCIFEESGELLGCEQHMMSRAASSQARDAIEVAMTKAERYYSLMG
                     LGSFSNGNAEGGLTTQEEKSLGAYTKSGDALIAGVILPTQQTPFPGLWLMDVVPDGEP
                     RFGYPNINDSSEALELIASGCHMVLFTTGRGSVIGSVIAPIIKVCNNPQTWEHLSDDM
                     DVNAGRVISEGATLDDIADDIMQVIEHVANGQYCAAERLEHQEFVLTYKAFDYSKSAV
                     CRADPR"
     gene            11864..12832
                     /locus_tag="QBH27_003533"
     CDS             11864..12832
                     /locus_tag="QBH27_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HDQ7015803.1"
                     /translation="MKKLLTPLLFLLALFSPMLNAAPVTIKIAYESNPGEPADTVVKH
                     WAELVKKKSNGDIVFELYPSSQLGSKQDITEQALMGMNIITISDVGFLTDYDADLGIL
                     FGPYLTEDPQKLFAIYASDWFKQKEQKLREKGVHIVMSNYLYGTRQIIAKKPIRTPED
                     LKGMKIRVPNNVMQIKAIEAMGATPTPMPLGDVYPALTQGMIDGVENPVSVLYGQKLH
                     EQAKYLSMVNYLTNTSLWLGGEAFFSTLTPAQLDIIHQTAYEAGLYSQKITTEQDATM
                     LKTMQEQGVEIIYPDVAPFKEKALKVYQQFPEWTPGLYDTIQQQLK"
     gene            12892..13353
                     /locus_tag="QBH27_003534"
     CDS             12892..13353
                     /locus_tag="QBH27_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDQ7015804.1"
                     /translation="MRLLGKMIEVAAVLTLFILVIMTIAAVIMRYCFHMPLQWTEEVS
                     GLLMIWVVMFGAVVAERDNTHLSIPFLADALTSRGQRILAFLVSLMSIILLVVMAWSA
                     WKLAAGTAFKTTQILKISWFWIDIAVTVGAIGVACFTALQLIKKTPRKEGE"
     gene            13353..>14660
                     /locus_tag="QBH27_003535"
     CDS             13353..>14660
                     /locus_tag="QBH27_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDQ7015805.1"
                     /translation="MTALSELLAGSWLDNWLMIIPLMFVCLFINMPVWLAMFCGILTY
                     FLCYSNVPLMVAIQRLIATTQTPSLLAIPFFILLGTLMSYTGIAARILQIANVLIGRM
                     RGGLGGANILVSTMMGGVSASNLADAAMLSRMMVPEMEQKGYSRGFAAAITASGSLIT
                     PIIPPGIALILYGLVADVSIGSMFMAGILPGLLCSAMLIVTVWLVAIRRGLKPSSEHW
                     PKPKEVSVALVQAWPALFLIVAVIGGIRANIFTPTEAGAVAVLLVMFIGFVIYRELKI
                     SHVFTALSETARSTASVMLIIMASAALGWIFSLERAGITVAEFVTSLTDNKYVFLLII
                     NLLLLVLGMFLEGSAILLILVPLLKPVVAHFGIDPVHFGIIMILNLSLGTLSPPVGTV
                     MLLVCNTVQVSVGQFMREAVPLLISLILLLLVITYIPAISLLLV"
BASE COUNT         3603 a         3434 c         4041 g         3582 t
ORIGIN      
        1 gcgcgcggtt tctgaactta agcgcctgat acagccaaat catcagtacc agtcctacac
       61 acgccagtgc gccccaggta atttgatcaa atacttcaat atatgcattg atggagtagt
      121 tgatcgcccc ggaagcatca aacgaagcct gcgatgtctg atcggcaatc acgcccgcca
      181 gatagttcgc aatcgcgccg gaaagcagca tataaatgcc ggttaatacg ccggtcacgc
      241 cggggatttc aatgcgcgta atctgcgaca tagcaaccgg gtcgataaac agttccgcaa
      301 agcccatcac cgccaggcct aacaccatca gtggcagaga agagtgacca tacattgcgg
      361 accagcgggc gcttaaggtc agaatgcaga atccggcgct catcaggcca aggccaagag
      421 caaattttcc ccagatgcgc acggtacgat tacctgcgac gctttcttta accacccacg
      481 ccaggaacac accgcacagc ataactgcga aggcattaat cgactggaac atagcagtcg
      541 gaacggtata accaaacata tcgcggttaa cgaagcggtc gatataaagg ctaatcgagc
      601 tacccccctg ttgtgcgaat gcccagaaca acatgctgaa gaaggtgagg gtaacaatca
      661 gccccagctc cttacgctgt ttttggtttt ctgctttgcg ataaattttt gccagaaccc
      721 caaggccaat gatcgtcgcg acaatcaagg cgtataccga ccactctttc cagaacaaca
      781 cggtaatcag cgcaggcgtt gccaccagca gaaccagtag ccatccccag ttgggcagga
      841 gaaagtttgt cgcacgcagt acctttttgt taacgccgcg ggtatgtgtg aaatgacgat
      901 tgccacataa gaaaatgacc agacccgcga tcatacccac cgctgccagg ccaaagccca
      961 tcgcccaact gtactcttcc tgggcgaaac cacaggcgat aggtgcgata atagacccca
     1021 cgttacccgc cgcatacatc agcgagaaac cgccatcacg acgtggatcg gttggctcat
     1081 acagctcacc gagcaggcaa ctgacgttgg atttaaacag accatagccg cagacgataa
     1141 tcgccaggga cagatagagg aatgacggat ggatctcact ggcacccagc accacatgac
     1201 cgatcgccat caacaacgcc cccagcatca ccgccatgcg attgccgaga actttatccg
     1261 ccaaaaagcc accgaggatt ggcgtgacat ataccagcga acagtaggcg ctaaataact
     1321 cgtaggcgtg attatcgcta tattttagtt gattggtgag atagaggatc agcagggcac
     1381 gcatgccgta aaagctaaaa tattcccaga tttgcagcgc aacgacatag taaatagcgc
     1441 gcggttgtga tgcgtgttta ttcataataa ttccacaatt gactgcaacg tcacgtaata
     1501 gttgcgcggc gtgcttgtgt ttccttaaag ttaaaacttt gatacagatc tgattattta
     1561 tgcaatatgc tgtctgattg cataaatata cattagctga agcgtgatga tataaacaat
     1621 ttgcgtcttt tggcgtcggg tttgtttctt aatatgtcaa aagattggct atcgacgaac
     1681 aggcgaggta acgctatgtt aacggtgtag ctatcagaat acggaaggcg cgaagatgaa
     1741 aaacaccgaa ctggaacaac tgattaacga aaaactgaac agcgcggcga ttagtgatta
     1801 cgcgccgaat ggtttgcagg tggaaggcaa agagatggtg caaaaaattg ttaccggtgt
     1861 aactgccagc caggctttgc tcgatgaggc agtgcgtctg ggcgcggatg cggtcattgt
     1921 gcatcatggc tacttctgga aaggtgaatc tccggtcatt cgcggaatga agcgtaaccg
     1981 tttaaaaacg ttgctggcga atgatatcaa cctgtatggc tggcatctgc ccctggacgc
     2041 gcatccggag ctgggtaata acgcacagct ggcggcgtta ctggggatca cggtcatggg
     2101 cgaaattgag ccgttggtgc cgtggggcga actgaccatg cctgtgccgg gactggaact
     2161 ggcctcctgg attgaagcgc gtctgggacg taagccgtta tggtgtggcg ataccggacc
     2221 tgaggtggtc cagcgcgtcg cctggtgcac ggggggtggg caaagtttta tcgatagcgc
     2281 cgcgcgtttt ggcgtggatg cttttattac tggcgaagtt tctgaacaga ccattcattc
     2341 agcccgcgag cagggattgc atttttatgc tgcgggtcac catgccactg aacgcggtgg
     2401 tattcgcgca ttgagcgagt ggctgaatga aaataccgat cttgatgtga cctttattga
     2461 tattcctaat cctgcataac gaataatcag agggatcgaa agtgcaacga gcgcgttgtt
     2521 atctgatagg tgaaacggcg gtagtactgg aactggaacc gccggtgacg ctggctagcc
     2581 agaaacggat ctggcgactg gcacagcgtc tggtggatat gccgaatgtg gttgaagcca
     2641 ttcctggcat gaacaatatc acagtgattt tgcgtaatcc tgagtcgctg gcgctggatg
     2701 ccatagagcg tttgcaacgc tggtgggagg agagtgaggc gctggagccg gagtctcgct
     2761 ttattgaaat tccggtagtt tacggtggcg cagccggacc ggatttggcg gtggtcgcgg
     2821 cgcattgtgg attgagcgaa aaacaggttg ttgaattgca ctcctccgtg gaatacgtgg
     2881 tctggttttt aggttttcaa ccgggcttcc cgtatctcgg gagtttgccg gaacaactac
     2941 acacgccacg gcgcgctgaa ccgcgcttgc acgttccggc aggttctgtc gggatcggcg
     3001 ggccgcagac cggtgtttat ccgctggcaa cgccgggtgg ctggcagttg attggtcata
     3061 cctcactcag cctgtttgat ccggcgtgtg acgaacccat cttattacgt ccgggagaca
     3121 gcgtgcgctt tgtaccgcag aaggagggag tatgctgaag attattcgtg cgggcatgta
     3181 caccaccgtg caggatggcg gccgtcacgg ctttcgtcag tctggtatca gccactgtgg
     3241 cgcactggat atgcctgcgt tacgcattgc taacctgctg gtgggtaatg acgccaatgc
     3301 ccccgcgctg gagatcacgc tcggtcagtt aacggttgag ttcgaaactg atgggtggtt
     3361 tgctctgacg ggtgccggtt gcgaagcgcg gctggatgat aatgccgtct ggaccggctg
     3421 gcgattgccg atgaaagcag gccagcgttt aacgcttaaa cgcccgcagc acgggatgcg
     3481 cagttatctg gcggtcgcgg gtggtattga tgttccgccg gtaatgggat catgcagcac
     3541 cgatctcaaa gtggggattg gcgggctgga aggccgttta ctgagggatg gtgaccgact
     3601 cccgattggc aaagcgaagc gtgattttat ggaagcgcag ggcgttaaac agctgctctg
     3661 gggcaaccgc attcgcgcct tgccggggcc ggaatatcat gagttcgatc gcgcctcgca
     3721 ggatgcattc tggcgttcgc cctggcagct tagcccgcaa agtaaccgca tgggctatcg
     3781 cttacagggg caaattttaa aacgcaccac cgatcgcgaa ctgttatctc acggtttgtt
     3841 gccgggcgtg gtgcaggtgc cgcataacgg ccagccgatt gtattgatga acgacgcaca
     3901 gaccaccggt ggttacccgc gtattgcctg tatcattgag gctgatatgt accatctggc
     3961 gcaaattccg ctcggtcagc cgattcattt tgtccagtgt tcactggaag aggcactgaa
     4021 agcgcggcaa gatcagcaac gttatttcga acaattagcg tggcggctgc acaatgaaaa
     4081 ttgacctgaa cgccgatctg ggcgaaggct gcgccagcga cgcagagcta ttaacgctgg
     4141 tttcctctgc caatattgcc tgtggatttc atgcaggaga tgcgcaaacc atgcaggctt
     4201 gcgtgcgtga agcaataaaa aatggtgtcg cgattggcgc tcacccgagt tttcccgaca
     4261 gggaaaattt tggtcgcagc gccatgcagc tgccgccaga aaccattttc gcccagacgc
     4321 tgtatcaaat tggcgcgctg gcagcgatta cccgtgcgca aggcggcgta atgtgtcatg
     4381 tcaaaccgca cggcatgtta tacaaccagg cggcgaaaga ggcacaactg gcagacgcta
     4441 tcgccagagc ggtatacgct tgcgatccgg cattgattct cgtcgggctg gcaggaagcg
     4501 agctgattcg tgcaggcgaa cgatatggcc tggtaacgcg cgaggaagtg tttgccgatc
     4561 gtggttatca ggcagacggt tcgctggtgc cgcgaagcca gccaggcgcg ttgattgaaa
     4621 acgaagaaca ggcgctggcg caaacgttgg agatggtgca acacggcaga gtcaaaagta
     4681 tcaccggcga atgggcaatg gtcacggcgc aaacggtctg cctacatggc gatggcgagc
     4741 acgcactggc gttcgcccgc cgattacgag ctacatttgc tgaaaagggg attgttgtcg
     4801 cagcataacc ccgcttaata aagaatgaaa aaaggatatc accatgcctg aaggcccgga
     4861 gatccgccgt gcagcggata acctggaggc ggcgatcaaa ggcaaaccac taactgatgt
     4921 ctggtttgcc ttcccgcagt taaaatctta tcaatcacga cttatcggtc aacacgttac
     4981 ccatgtggaa acgcgtggta aggcgttgtt aactcatttt tccaacgact taacgctcta
     5041 cagccataat cagctttacg gcgtctggcg cgtggttgat accggcgaag agccgcagac
     5101 cacgcgagta ttgcgggtaa aactgcaaac ggctgacaaa accattctgc tttatagcgc
     5161 ctcggatatt gagatgttga ctccggaaca actgaccacg catccgtttt tacaacgcgt
     5221 tggtcccgat gtgctggatc cgaatctgac gccggaggtg gtaaaggaac gattattgtc
     5281 gccgcgcttt cgtaaccgtc agtttgctgg attactgctc gatcaggcgt ttctggcagg
     5341 acttggcaat tatttgcggg tggagatcct ctggcaggtt gggttgaccg gaaatcataa
     5401 agcgaaagat ctcaatgcgc cgcaactgga tgcactcgca cacgcgttac tggatattcc
     5461 gcgactttct tacgctacgc gggggcaggt ggatgaaaac aaatatcatg gggcgctgtt
     5521 tcgctttaag gtttttcatc gtgatggcga accttgcgaa cgctgtggcg gcatcattga
     5581 gaaaaccacg ctgtcatcgc gcccgtttta ctggtgccca ggctgccagc actaggccga
     5641 acgcttcggc gcataggttg aaataaaccg tgcgatggca ggccccgtca gcaaaatact
     5701 gaacaggcgc agggtttgca tcgcaatgat gagcgccata tcggcattgc tccctgcggc
     5761 gatgaccgcc acggtatcaa gcccgccggg gctggtggcg aggtaggcgg tcataaaatc
     5821 aatatgcata aaccgggtca gcccccacgc catgcccgca caaatagcca gcagagcaaa
     5881 aatcgacagc aggatttgcg gtagcgggcg caatgcccgc agtaatattt gcttatcgaa
     5941 accaagacca atccgccagc caattgccat atatgccatc gccagcagcc attccgggag
     6001 ttcaatcgtg atgagctgac cagactggag cactgcgccc gccagcatcg ggatgagcat
     6061 cgtgccggaa ggaagacgta gcaagcatcc cgccgtaccg gcaacgaccg cgagcaaaat
     6121 cgtaagcagg agattaatgc tcactggcgg gaaccagacg atctgctggt taaccgcttc
     6181 agcgttatcg cccagcatca tgcgggtgac cagaacagcg gctcctgcga cgaacagcac
     6241 tcgcagatat tgcataaacg ccaccaggcg aatatctgcg ccgtaatctt gcgccattgc
     6301 gaccattgcc gccgcgccgc caggagaaga accccaggcc ccggtattcc caggcagtga
     6361 gctatagcgc accaataacc aacccacgat ggcgctggaa agcagcgtca ccagtaaaat
     6421 cgcaagcacg atcggccagt tgacggccag cgtggtgaga atcgaaccag tgaggttttg
     6481 tgcaatcatg cagccaagaa tggcttgggc agcgagaaaa gcggagcggg gaagttgcag
     6541 agtgactccg tgcatactga agatgatccc cgcgatcatc gggccaagca ggagtgccgc
     6601 cgggagatgt aaagcgagga agccaataga aaggagaagt gacagaacac ataacattcc
     6661 ccactgtagc actggcatcc gtcactccct gtttaactta cctttggtgt atcgatacag
     6721 cataaagaat gcgcctcaac gaaaaaatag cggtaatcaa gaaaaagaca gattttggct
     6781 aaagggcgta ttaagtaccg ttgtctccct ctcgtttgta aaagagggaa gagtaaacga
     6841 ttgaactgag ataagtctta tgacgaggaa ttattgatca gtgcactgac cgggctgtcg
     6901 aagtgtgatt tcatggcttg agtcgccgtt tcgacatccc tggcacacac agcatcgcag
     6961 attttctggt gtagtgtgat gatgtgattg ccatactctt tctgatcgct ttgggcgttt
     7021 aaaccctcct ggatggattg ccgcaactgc tgatgtaatg cctgcaataa catcataaaa
     7081 agcggatttt ttgaggcaac ggcaagtaac tgatggaagc ggatatcatg ggtgataaag
     7141 ctttcaacat cattgaactg ttcgcggatt tttgccattt cagcccgtag gttagcaaaa
     7201 tcttcctctt cgccattacg ggcagcagct tccacaacgc ccaactccag ccagcggcgt
     7261 acttgaagaa tttgctctgt tgtcacttgt tcggtcgcca gggcaaatcc aaacaccacg
     7321 cgcagcactt catcatcaaa ttttttgatt cgtggcactt taccgctgtg cgtttccaga
     7381 atccccaggg cggcaagtga acgataggcc tcacgcacaa taccccgact gacacccaat
     7441 ttctgaatca gctgaacttc actgggaact ggcatccccg gtgtcaattt ttgttcggcg
     7501 atcagattaa gaatatgatt tttgacctgt gacgataacg ttacgctggt caataacgca
     7561 ctctcactca tttttactcc tgttgtactt tttcctggtc ggtgaaatcc agaataccgg
     7621 gactgtatcc ttcatatttt cctccagaac ccttgattat caatagacta ctgcgcctgt
     7681 gcgcccgcca tgcagtgcga atcaaagaca tattgtgtga aagagtggcg aagaaatagt
     7741 aaatacacaa ctttttcgtg acgcccttcg cagtattgtg cgctttttct ccttaccctg
     7801 taaacctgtt aaacaggctt acaggacgaa aataatgtgg aagagtgata acgaactttt
     7861 tgctttgatg aaaacccggc tatttaccgc cgtggttggc gatattctcg acacgctggg
     7921 ctatatgcac cagtttgttt ctccggcaat caagccgctg gatgtgaata cggtggtggc
     7981 aggccgggca atgccggtgc tggagtcgga cgtttacgaa accaaatcga cgtcgggtaa
     8041 gacggcgatt ggcgaaaaac cgtttggcat catgtttgaa gcgctggatg ccctcaaaga
     8101 gaacgatgtt tacatcacta cgggcagctc tctgcgctat gccttgtggg gcgggttgat
     8161 gtctacccgt gccatgcatc tgaaagcagc aggtgcggta ctcgatggtt acgcccgtga
     8221 ctctgctgaa attcttgaat tgaaatttcc cactttttgt catggcactt acgcacagga
     8281 tcaaggtccg cgcggcaagg tcatcgactt cggcgtacct attgaatttc acggcgtgcg
     8341 agtgaatccg ggcgatatcg tgtttggtga tcgtgacgga ttactgatta ttccccaggt
     8401 tgttgagaaa gaagttatta cccaggccct ggaaaaagtg gcaactgaaa gtgaagtgcg
     8461 caaagccatc agtgatggca tgagcacggt acaggccttt gaaaccttcg gcgtaatgta
     8521 aggagagcaa tgaatgaagg cattaaccat tgagcaggga agtcaggcgc gatttgttga
     8581 tatccccgaa cctgtctgcg gtaaaggaga agtgctaatc aatgttgagc gtgtcggact
     8641 gtgtggttct gacctcaata cctggcgggg actaaatccg ctggttaatt atccctgtat
     8701 tccggggcat gaaattggcg gcacggtgct ggcaactggc gatatggcag gggcgatcaa
     8761 gccaggtgac cgggtggtcg ttctgccttg cactgagtgt ggcgtgtgca gctcctgtct
     8821 gagcggtcgt cctaacgcct gtaaaaacaa ccaaactctt ggtgtgcaac gtcagggggg
     8881 gatggcgcaa caactgacga tgcctgcaca taaagtcatt gtctgtaatg ccttaagtgt
     8941 tgatgagtta gcgctggtag aaccgctggc cgtgggacta catgcggcca gacgtgcgca
     9001 aattaatgtg ggtgaatggg tcgcagttat cggttgtggg gtaattggcc ttggcgctat
     9061 cgccgcggca tccgccatgg gggcgcgcgt aattgctatc gatattgatg atcgcaagcg
     9121 gaacattgcc ctggaatgcg gtgcgcaacg ttttcttaat tcgcagcaat gtgacgtggc
     9181 ggcagaactg gtgctgttaa atgacggtcg cggcccggaa gtggtgattg aagcggtggg
     9241 taatcaacaa accattgaac aggctacagg gttggtggcg tttagcggtc gtgtggttta
     9301 tgtcggttac gccaaacagc cagtgagtta tcagagtgcg cagttcctga tgaaagaact
     9361 ggatatccgt ggttcgcgca atgccaccgt ggcggatttt cgtgccgtgc tggcattgat
     9421 ggagtccggg cgttatccgg tcagcaatct ggtgagtgct cactacccgc tggaacaggc
     9481 tgatcacgct tttagcgact gggcaagtga tcccggcgcg tataccaaaa tcctcattga
     9541 cctttaagga gcgacaccat gaatgctttt tctttacata acaaaaccgc gttgatcacc
     9601 ggtgcagcca gtgggctggg gctggcaatg gcgaaatgca tgatagtcag cggggcgaag
     9661 gtcattattg ctgacctgaa cggcgagctg gcgcaagcga ccgccggaac attggggtcg
     9721 caggccagtt ggatacaaat ggatgtcggg gataccgcag ccgcgcagga aaaagttgat
     9781 gtgcttatta gcgaacacgg ggctatcgat atcctggtca ataacgcagg caatcactgc
     9841 aaaaaaccga ttgaagagat gagcgtcagt gattttgagt cagtactcga cgttcacgta
     9901 gtcggcgcat ttgcgttaac caaagcgctg gtgccgcata tgaaacaacg cggtaaaggc
     9961 tgtgtgcttt ttactgcatc gatgacctca tttcttgggc agcctctggt tactggctac
    10021 agcgcagcga agtcagccta tctcggcatg attcgcggta tgaccacgga gcttgccgtt
    10081 catgggattc gcgtcaatgg tattgcgcca ggctggatcg atacaccaat gttgcgtaag
    10141 gccattgaag gtgatgacga acgccgtaac aaaatcctgg gtcgtacccc gatgaaaaaa
    10201 ttcggcaagc cggaggatat cggttgggct gcggtttatc tggcttcaga cgcagcggcg
    10261 tttgtcagtg gtcaggtgct ggtggtggat ggcggcgcat tgatcggctt ctgatgtacg
    10321 gaggaattat gcgcaaacgt gcgttaaagc tacatgccaa agataactgt gtagtcgcgc
    10381 tgaatgacat cgccagtggc gatattgtct ggtgggatga gggggaaatg gtggcctgta
    10441 gttccgttac cctggggcac aaactggcct gcgaagcgct gaacaatggt gattcgattc
    10501 tgaaatacgg cgccattatc ggacacgcca ctcgcgatat tgccatgggt gaacatgtgc
    10561 attcccataa tcttgccagc aatgccatcg ccatttttca tcatgatgat gccggctatc
    10621 agtcagagga aaaagcataa tggaaggtta tgtacggcaa aatggtcgac caggtattcg
    10681 taatactgtg caggtaatct ttatggtgga gtgtgccagc catgtcgcta aaaagattgc
    10741 cgaaggattt ccttgtcacg acgtgcagta ttttggtttt tcaggttgtt atcccagcga
    10801 ttatggctat cggctggtga aaaatctggc gactcatagc aacgtaggtg gtgtactgat
    10861 tatttcgctg gggtgcgaga atttcgatcg ccgtcggctg gagcaagaag tacgagaaag
    10921 tgggcgaccg tgccatacgc tggtcattca ggaaaataaa ggcacgacca acaccattac
    10981 tctgggtaag cagttggtcg ctgaaatgct ggaacagctt gcggatacac cgcgttgtat
    11041 cctgaactgg agcgacctgg tggttggtac tatctgcggt ggctccgatg gcaccagtgg
    11101 tatcacgggt aacccggcag taggacgtgc ctttgaccaa ctgctggaga aaggggctac
    11161 ctgtattttt gaagaaagcg gtgagttgct tggttgtgag cagcatatga tgtcgcgcgc
    11221 ggctagttcg caggcacgtg atgccatcga agtggcaatg acaaaagccg aacgttatta
    11281 cagtttgatg gggctgggga gtttttctaa cggtaatgct gaaggcggct taaccactca
    11341 ggaagaaaaa tcccttgggg cttataccaa gtcgggcgat gcgctgattg ctggcgttat
    11401 tttgccgacg caacaaacgc cctttcctgg gctttggttg atggacgtgg tgcctgatgg
    11461 tgaacctcgt tttgggtatc cgaatattaa cgattcctca gaagcactgg aacttatcgc
    11521 ctctggctgt catatggtgt tatttaccac aggtcgcggt tcagttattg gttcagtgat
    11581 cgctcctatt attaaagtgt gtaataaccc gcagacgtgg gagcatttat ctgacgatat
    11641 ggacgttaat gccggtcgtg taattagcga aggcgcaacg ctcgacgata ttgcagacga
    11701 tattatgcag gtgattgaac atgtcgccaa cggtcaatat tgtgccgcag aacgactgga
    11761 acatcaggag tttgttttaa cgtataaagc atttgattac agcaaaagtg cagtctgtcg
    11821 ggcagatccc cgttaatcga taaagctctt acaggtaata ataatgaaaa aattactgac
    11881 accactttta tttcttttag ctttatttag tccgatgctg aatgccgcac cggtaactat
    11941 caaaatcgcc tatgaaagta accccggcga gccagcggat acagtagtga agcactgggc
    12001 agaacttgtg aagaaaaaaa gcaatggaga tattgttttc gaactttacc caagttcaca
    12061 gttaggttct aaacaagata ttactgaaca agcgttgatg gggatgaata ttatcaccat
    12121 cagtgatgta ggatttttaa ctgactacga tgcagacttg ggaatattgt ttgggcctta
    12181 tttaaccgaa gatccgcaga aactgtttgc gatttatgcc agtgactggt ttaaacaaaa
    12241 agaacagaaa ctacgggaaa aaggtgtgca tattgtcatg agcaactatc tgtacggtac
    12301 caggcagatc attgctaaaa aaccgattcg tacacctgaa gacttgaaag ggatgaagat
    12361 ccgcgtgccg aataacgtca tgcagattaa agctatcgaa gcgatgggcg caaccccaac
    12421 accaatgccg ctgggagatg tttatccggc gctgactcag gggatgatcg atggtgtgga
    12481 aaacccggtt tctgttctct atgggcaaaa gctgcatgag caggcgaaat atctgtcaat
    12541 ggtgaactat ctgaccaata catcattatg gttgggtggc gaagcgtttt tcagtaccct
    12601 gacacctgcg cagttagata ttattcacca gacagcgtat gaagctggtt tatatagcca
    12661 gaaaattacc actgagcagg atgcgacaat gttgaaaacc atgcaggagc agggcgtgga
    12721 aatcatctat cctgacgtcg cccctttcaa agaaaaagcc ttaaaagtct atcaacaatt
    12781 cccggaatgg acccctggtt tgtatgacac tattcagcaa cagctgaaat aacttcatgg
    12841 cataaggact ggtcggtgtt tcggcaccga ccgttaagag cagaggtgtt tatgcgtctt
    12901 ttagggaaaa tgattgaagt tgctgcggta ttaacgctct ttatattggt aatcatgacc
    12961 attgctgcgg taataatgcg ttactgtttt catatgccat tacagtggac agaagaggtt
    13021 tccggtttat taatgatttg ggtagtcatg tttggtgcgg ttgttgctga acgagataat
    13081 acccatttat cgattccatt tttagccgat gcacttactt cacgcgggca acgtatatta
    13141 gcgtttttag tctctctgat gtctattatt ttgctggtgg tcatggcctg gtcagcatgg
    13201 aaattagctg ccgggacagc ctttaaaacc acgcaaatat taaaaatatc ctggttttgg
    13261 atcgatattg ccgtcacggt tggcgctatc ggtgtcgcct gttttactgc cttacaactg
    13321 atcaagaaaa cgccacgcaa agaaggtgaa taatgacagc attgagtgaa ttactggcgg
    13381 gaagctggct ggataactgg ctgatgatca ttccgcttat gttcgtctgt ttatttatca
    13441 atatgccggt atggctggcg atgttttgcg gcattctgac ctacttcctt tgctattcaa
    13501 atgtgccgtt aatggtggca attcagcgtt tgattgccac gacgcaaacc ccttcgctgt
    13561 tagctattcc gttttttatc ttattaggca cgttaatgag ctataccggt attgccgcgc
    13621 gtattttgca aattgccaac gtgttgattg gcagaatgcg cggtggcctg ggtggggcta
    13681 atattctggt cagtaccatg atgggcggtg tcagtgcctc aaatctggcg gatgccgcaa
    13741 tgttatcgcg catgatggtg ccggaaatgg aacagaaagg ttattcccgt ggctttgcgg
    13801 cggcgataac ggcaagtggt tcgctgatta caccaattat tccaccgggt attgcactga
    13861 tcctttacgg actggtggcg gatgtctcca tcggctccat gtttatggcc gggatcctgc
    13921 caggtctgct gtgctccgcc atgttgatcg ttaccgtatg gttggtggct attcgtcgcg
    13981 gtttaaaacc ttccagcgaa cactggccaa aaccgaaaga agtgtcagtg gcgctggtgc
    14041 aggcctggcc agcactgttc ttaattgttg cggttatcgg tggtattcgg gcaaatatct
    14101 ttacgccaac ggaagccgga gcggtagcag tattattggt aatgtttatt ggttttgtaa
    14161 tttatcgcga actgaaaatc agccatgtat ttacagcact ctctgagacg gcgcgttcca
    14221 cggcatcggt catgttaatc attatggcga gtgccgcgct gggctggata ttttcccttg
    14281 agcgggcagg tatcacggtt gcggagtttg taacttctct gaccgataat aaatacgtct
    14341 ttttattgat tatcaatcta ttgctgctgg tgcttggcat gttccttgaa ggtagtgcca
    14401 ttctgctgat attagtccca ttacttaaac ccgtggtggc tcattttggc attgatccgg
    14461 tgcattttgg catcattatg atcctcaact tatcgttagg aacgttatca ccacctgtcg
    14521 gaacggtgat gctgctggta tgtaataccg tacaggtttc tgtcgggcaa tttatgcgtg
    14581 aagcggtgcc attactgatc agtcttattt tacttctgtt ggtcattacc tatattccgg
    14641 caatatcttt gctgctggtg
//
LOCUS       DAOFSV010000116        14413 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.116, whole genome shotgun sequence.
ACCESSION   DAOFSV010000116 DAOFSV010000000
VERSION     DAOFSV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.116"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            94..2028
                     /gene="yeaG"
                     /locus_tag="QBH27_003536"
     CDS             94..2028
                     /gene="yeaG"
                     /locus_tag="QBH27_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDQ7015806.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2141..3424
                     /locus_tag="QBH27_003537"
     CDS             2141..3424
                     /locus_tag="QBH27_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDQ7015807.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            3571..5046
                     /locus_tag="QBH27_003538"
     CDS             3571..5046
                     /locus_tag="QBH27_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ7015808.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            5227..6717
                     /locus_tag="QBH27_003539"
     CDS             5227..6717
                     /locus_tag="QBH27_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ7015809.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            6760..7263
                     /gene="yeaK"
                     /locus_tag="QBH27_003540"
     CDS             6760..7263
                     /gene="yeaK"
                     /locus_tag="QBH27_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDQ7015810.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(7264..7368)
                     /locus_tag="QBH27_003541"
     CDS             complement(7264..7368)
                     /locus_tag="QBH27_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015811.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            7538..7984
                     /locus_tag="QBH27_003542"
     CDS             7538..7984
                     /locus_tag="QBH27_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDQ7015812.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(7941..8762)
                     /gene="nimR"
                     /locus_tag="QBH27_003543"
     CDS             complement(7941..8762)
                     /gene="nimR"
                     /locus_tag="QBH27_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDQ7015813.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPGSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            8859..10040
                     /gene="nimT"
                     /locus_tag="QBH27_003544"
     CDS             8859..10040
                     /gene="nimT"
                     /locus_tag="QBH27_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDQ7015814.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            10095..10442
                     /locus_tag="QBH27_003545"
     CDS             10095..10442
                     /locus_tag="QBH27_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDQ7015815.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWNKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(10464..10718)
                     /gene="yoaF"
                     /locus_tag="QBH27_003546"
     CDS             complement(10464..10718)
                     /gene="yoaF"
                     /locus_tag="QBH27_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDQ7015816.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            10901..11926
                     /gene="dgcP"
                     /locus_tag="QBH27_003547"
     CDS             10901..11926
                     /gene="dgcP"
                     /locus_tag="QBH27_003547"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDQ7015817.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            11959..12057
                     /locus_tag="QBH27_003548"
     CDS             11959..12057
                     /locus_tag="QBH27_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDQ7015818.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            12060..12134
                     /gene="yoaJ"
                     /locus_tag="QBH27_003549"
     CDS             12060..12134
                     /gene="yoaJ"
                     /locus_tag="QBH27_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDQ7015819.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(12193..12441)
                     /locus_tag="QBH27_003550"
     CDS             complement(12193..12441)
                     /locus_tag="QBH27_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDQ7015820.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(12589..12771)
                     /locus_tag="QBH27_003551"
     CDS             complement(12589..12771)
                     /locus_tag="QBH27_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDQ7015821.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(12775..13134)
                     /gene="yeaR"
                     /locus_tag="QBH27_003552"
     CDS             complement(12775..13134)
                     /gene="yeaR"
                     /locus_tag="QBH27_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDQ7015822.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(13307..13945)
                     /gene="leuE"
                     /locus_tag="QBH27_003553"
     CDS             complement(13307..13945)
                     /gene="leuE"
                     /locus_tag="QBH27_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDQ7015823.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(14072..14404)
                     /locus_tag="QBH27_003554"
     CDS             complement(14072..14404)
                     /locus_tag="QBH27_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7015824.1"
                     /translation="MSLTPNALDTTTSKYRFLNDDFILCSFIHSTAWRGIFAFGVFPD
                     HVKIDVARLFPASGHLVQVLPEYYQPANVWAVYVSKLSTSAKVRITVEFLRQYFAEHY
                     PNFSLEHA"
BASE COUNT         3759 a         3430 c         3381 g         3843 t
ORIGIN      
        1 aagagacgtc gttcagttta cctcttccgg gagcctctac tattcatatg aacggctctt
       61 aacctgtgct aaaaaacgaa aggacggcat accatgaata tattcgatca ctatcgccag
      121 cgatatgaag ctgccaagga cgaagagttc acactgcagg agtttcttac cacttgtcgg
      181 caagatcgca gtgcttatgc caacgcggct gagcggctat tgatggctat tggtgagcct
      241 gtcatggtcg atacagccca ggaacccaga ctttctcgac tcttttctaa ccgggtcatt
      301 gcacgttatc cggcgtttga agagttttac ggcatggaag acgcgattga acagattgtt
      361 tcttatctga aacacgcggc tcaggggctg gaagagaaga aacaaattct gtatctgctg
      421 gggcctgtgg gtgggggtaa atcatcactt gctgagcgac tgaaatcatt aatgcagctc
      481 gtaccgattt atgtattgag cgccaacggt gagcgtagcc cggtcaacga tcatccgttc
      541 tgtcttttca atccgcagga agatgcgcag attctggaaa aagagtacgg cattcctcgc
      601 cgttatctcg gcaccatcat gtcgccgtgg gcggcaaagc gcctgcatga atttggtggc
      661 gatatcacta agttccgggt agtgaaggtc tggccgtcaa ttctgcaaca aattgccatc
      721 gccaaaacgg aacccggaga tgagaacaac caggacatct ccgccctggt agggaaagtc
      781 gatattcgta aactcgaaca ctacgcgcag aatgacccgg acgcctacgg ctattccggt
      841 gcgctgtgcc gcgccaatca ggggatcatg gaatttgtcg agatgtttaa agcaccgata
      901 aaagtgctgc atcccttgtt aaccgccacc caggaaggta actacaacgg gacggaaggt
      961 atctccgccc tgccgttcaa cgggattatt ctcgcccact cgaacgaatc cgaatgggtc
     1021 actttccgta ataacaaaaa caacgaagcc ttcctcgacc gtgtttacat cgtgaaggtg
     1081 ccgtattgct tgcgcatttc cgaagagatc aaaatctacg agaaattgct taatcacagt
     1141 gaattgactc acgccccctg cgcccctggc acgctggaaa cgctgtcacg tttttctatt
     1201 ctttcgcgtc tgaaagagcc agaaaactcc agcatttatt caaagatgcg cgtttatgat
     1261 ggtgaaagtc tgaaagacac tgatcccaaa gccaagtcgt atcaggaata tcgtgactac
     1321 gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt ttgcgtttaa gatcctctcc
     1381 cgcgtgttca acttcgatca tgtagaagtg gcggcaaacc cggtccatct gttctacgtc
     1441 ctggaacagc agatcgagcg cgagcagttc ccgcaagagc aggctgaacg ctatctggag
     1501 ttcctgaaag gttatctgat cccgaaatat gccgagttta tcggcaaaga gatccagacg
     1561 gcctaccttg aatcctattc agaatatggg caaaacattt tcgaccgtta tgttacctac
     1621 gcggatttct ggattcagga tcaggagtat cgcgatccgg ataccgggca gctgtttgac
     1681 cgcgagtctc ttaacgccga gctggagaaa atcgagaaac cggcggggat cagtaatcca
     1741 aaagatttcc gcaacgagat tgttaacttc gtactgcgag ccagagcgaa taacagcgga
     1801 cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg ttatcgagaa gaaaatgttc
     1861 tccaataccg aagagctgtt gccggttatt tcgtttaacg ccaaaacgtc aaccgacgag
     1921 cagaagaaac acgacgactt tgtcgaccgt atgatggaaa aaggctacac ccgtaaacag
     1981 gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat cgtcttaaca accctggccc
     2041 ggtacacatg ctaccgggcc tacaacgaca gcgaaccgtg ggccagagaa gcggcaacac
     2101 aagcgtagca taccgttggc aaatgtagta cggggggcat atgacctggt ttattgaccg
     2161 gcgtctgaac ggcaaaaata aaagcatggt gaatcgccag cgtttcttac gccgttataa
     2221 agcgcaaatt aaacagtcga tctccgaggc cattaataag cgttcggtga ctgacgtcga
     2281 cagcggcgag tccgtatcca ttcccacgga agatattagc gaaccgatgt ttcatcaggg
     2341 gcgtgggggc ctgcgccacc gtgtgcatcc gggcaatgac catttcgtcc agaacgaccg
     2401 aattgaacgt ccccagggtg gtggcggagg ttccggcagt ggtcagggcc aggccagcca
     2461 ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg aaagatgagt atcttgatct
     2521 gctctttgaa gatttggcct taccgaatct gaaacaaaac caacaacgcc agctgaccga
     2581 atataaaacg catcgggcgg gttataccgc taacggcgtt ccggccaata tcagcgttgt
     2641 acgttcattg cagaactcac tggcgcggcg cacagctatg acggcgggca agcggcggga
     2701 acttcatgcg ctggaagaga atttggccat catcagcaac agtgaacctg cgcaactgct
     2761 ggaagaggaa cgtctgcgca aagaaattgc agaattacgt gccaaaattg aacgcgtccc
     2821 tttcattgac accttcgatt tacgttacaa gaactacgaa aaacggcccg atccctccag
     2881 ccaggcagtg atgttttgcc tgatggacgt ttccggttca atggatcagt ccactaaaga
     2941 tatggctaaa cgtttttata ttctgctgta tctgttcctc agcagaacgt ataagaacgt
     3001 ggaagtcgta tacatccgcc atcataccca ggcgaaagaa gtcgatgaac atgagttttt
     3061 ctactcgcag gaaactggcg gcaccattgt ttccagcgcc ctaaaactga tggatgaggt
     3121 agtgaaagag cgttataacc cggcacagtg gaatatttac gcagcccaag catcagacgg
     3181 cgataactgg gccgatgact ctccgctttg ccatgaaatt ctggcgaaaa aaatattacc
     3241 cgttgttcgt tattacagct atatcgaaat tacacgtcgc gcgcatcaga cactgtggcg
     3301 agaatatgag catctgcaat ctactttcga caactttgcg atgcagcata tccgcgacca
     3361 ggatgatatt tatccggtgt tccgtgaact gtttcataaa caaaatgcaa cagctaaaga
     3421 ctaaaactgt cagccgggtc attatcgcct ggctgatttt tagctcactg taaattatct
     3481 cttttattac atacattagc ttacaatcgc tttaaatatg acagcataac ctttacacaa
     3541 tttagttcca gaaaacaatc attcggaaaa atgattcggt caacacgtat ttccctgggg
     3601 ttattcttta attatttttt atcgttaacg aaaattgatc ttggtcaaaa ctatatatct
     3661 ctgccatcaa taaaatccag cactcacatt gctctccttt ttatggcttc tatgggtaca
     3721 caaaaattaa aagctcaagg cttttttatt ttcagcttat tgctgacgtt aattttattt
     3781 tgcattacta ccttatataa cgaaaacacg aatgtaaaac tcatcccaca gatgaattac
     3841 ctgatggttg ttgtggcttt gtttttcctt aacgccgtca tttttctttt catgttaatg
     3901 aagtatttca ctaacaaaca aattttacca acactcattt taagccttgc atttttaagt
     3961 ggccttatct atttagttga aaccattgta attatccata aaccaatcaa cggcagtaca
     4021 ctgatccaga caaagtcgaa tgatgtttct attttctata ttttccgcca actcagtttc
     4081 atttgtttaa cctcgctggc gctcttttgt tatgggaaag acaacatcct tgacaacaat
     4141 aagaaaaaaa ctggcattct gttgctggcg ctgattcctt ttttagtttt tccccttctg
     4201 gcacacaatc tgagcagtta taacgctgat tattctttgt atgtcgtcga ttactgcccg
     4261 gacaaccata ctgcgacctg gggaatcaac tatacaaaaa tattggtttg tttgtgggca
     4321 tttttactgt tctttattat catgcgtaca cgattagcca gcgaactatg gccgttaata
     4381 gcattattat gtctggcatc gctatgctgc aacttacttc tactgactct ggatgagtat
     4441 aattacacca tctggtatat cagtcgcggg attgaagttt ccagtaaact gtttgttgtg
     4501 tcttttctga tttataacat ttttcaggaa ctgcaactct ccagcaaact ggcagttcat
     4561 gatgtgctga ccaatattta taatcggcgc tactttttca acagcgtaga gtcattattg
     4621 tcgcgacctg ttgttaagga cttttgtgtc atgctggttg atattaatca gttcaaacgc
     4681 atcaatgccc aatggggaca tcgtgtgggt gataaagtgc tggtatcaat tgtcgatatt
     4741 atccagcaaa gcatccgccc cgatgatatt ttagcgcgac tggagggtga ggtgtttggc
     4801 ttgctattta ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg tatgcgtaaa
     4861 aatgtcgaac tcctgaccgg ctttagtaac agatatgatg ttcctgaaca aatgaccatc
     4921 agtattggca cggttttttc aacgggtgac acgcataata tctcgcttgt catgacggaa
     4981 gcagataaag ccttacgcga agcgaaaagc gaggggggca acaaagtgat tattcatcat
     5041 atttaagtgc aaaaatattc acagccatgc tttttgcgtg gcttttgcat acaatttatt
     5101 actattaccc ctaaatttcc cctccccagc ggcgcagaga tgagtataat tagcgcccct
     5161 gtgccaggcc gcaatcgaac tttatctggt tttctcgttt cactaaccga aggagtgcca
     5221 tttatcatga aattgcacca tagaatgctc cggcatttta tcgccgcaag tgtcattgtg
     5281 ctgacatctt ccttccttat ttttgaactt gtcgccagcg acagagcaat gagtgcctat
     5341 ctgcgctata tcgtgcagag agcagactct tcctttcttt atgataagta tcagaatcag
     5401 agtattgccg cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt accgccagaa
     5461 caacggcgcg ctatctgcga ggcttttgag tctgccaata atacccatgg cttaaacctg
     5521 actgctcata aataccaggg cttacgcggc acactacaaa ccgcatccac tgactgcgac
     5581 acaattgtgg aagctgcagc actattaccc gcttttgatc aggcagtgga aggcaaccgc
     5641 caccaggatg attacggttc aggtcttggg atggccgaag agaaatttca ctattatctc
     5701 gatctcaatg accgctatgt ctatttttat gagccggtta atgttgaata ctttgcgatg
     5761 aataactggt ccttcctgca atcaggaagt attggtatcg atcgcaaaga tattgaaaaa
     5821 gtatttaccg ggcgtaccgt attatcgagc atttaccagg atcagcgtac taaacagaac
     5881 gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc taaaagggat tgtgctgctg
     5941 gatattaaca aaaacaatct gcggaatatc ttttacactc atgaccgccc tctcctctgg
     6001 cgttttctca atgtcacgct aaccgataca gattcggggc gcgacattat catcaaccag
     6061 agcgaagata atctgttcca gtatgtcagt tacgtccatg acttaccggg cggcattcgt
     6121 gtctcgttat ccattgatat tctttacttt atcacgtctt cgtggaaaag cgttctgttc
     6181 tggattttga cggcgttaat tttgctgaat atggtgcgga tgcacttccg cttataccaa
     6241 aatgtgtcgc gagaaaatat tagtgatgcg atgactggac tctataatcg caaaatttta
     6301 acccctgaac tggagcagcg gttgcagaaa ctggtgcaat ccggttcttc ggtgatgttt
     6361 attgctattg acatggacaa gttaaagcaa ataaatgaca ccctcggtca tcaggagggg
     6421 gatttagcga ttacgttatt agctcaggcg attaaacagt cgattcgtaa aagtgattat
     6481 gccatccgac tcggtggcga tgaattctgc atcattcttg tcgattcgac gccgcaaatt
     6541 gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc gcagaaagag
     6601 atcggcttct cttccggtat ttacgcgatg aaagaaaacg atacgttaca tgatgcgtat
     6661 aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa
     6721 ccttctgtgg ttgtttgctt gtaatctcag gagcatgaaa tgactgaaat ggctaaagga
     6781 agcgtgacgc atcagcggtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc
     6841 gttacccatg aagcggtagg taaatgtgaa gcagtgtcag aaattcgtgg caccgcttta
     6901 ggccaggggg ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt
     6961 ctggcgatcc tcgctgccga tcaacaggcc gatctgagcc aactcgccag ccatataggc
     7021 ggattacgcg cttcgttggc cagcccggcg gaagtcgatg aactcacagg ctgtgtcttc
     7081 ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga ccccctactc
     7141 ttcgaacgat ttgatgaaat cgccttcaat gcaggcatgc tggataaatc cgttattttg
     7201 aaaaccgcgg attatttgcg tattgcgcaa ccagaactgg tcaatttccg ccgcactgcg
     7261 taactagcct gtccgttcaa taagcagaac ggacaaaacc agcacagcag caatggcaaa
     7321 aaacgatgac gtgataatca gtgtctcgac gaacatttga tcgttcatag tatgcccccg
     7381 ggagatagct gttttcacgt tattgttaac gtacacaaat ggcagtttga tgacagttcg
     7441 tctgattttt tatgcaaaaa acgtaaagat tttctacttc ctgcctgcag caagcgtaaa
     7501 gtaagcaggc ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat
     7561 cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat
     7621 tctggtgttg attattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa
     7681 acaggggcta agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag
     7741 cgggacgcta ccgccctcaa ccctgattca ctcgtttctg aactggaaat cgctggtggc
     7801 aattgcagtc ggcgttattg tctcctggct gggggggcgc ggcgtgacgt taatgggcag
     7861 ccagccgcaa ctggtcgccg ggctgctggt gggcactgtt ttaggcgtgg cgctgtttcg
     7921 cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca
     7981 gtagttaatc ccgcaatata tcggcctggc gtttgcccca ggcctttctt aaacatagta
     8041 ataaaggccg ttgttgaatc ataccccagt gtatgagcaa ctttctgtac agtatcgcct
     8101 ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc
     8161 aaccccgtct ctttcactat cagccgggca agattgcgtt cactcatcgc aaaaaagcca
     8221 gcccattgcc tcaatgagcc ccactcgacg ggctctttcg ccatcatctc caccatcgtg
     8281 cggattttag gatgagaaga accgggcaag tgcagctgtt gttgtggttg ctgagggagt
     8341 tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc
     8401 ctgtttgcca gggttaaaat taattcccgg cataagggcg atattttcaa tgtgcaacat
     8461 gttgtcggca ttgtcactgc gctgggctcg ataaacagaa aacagagttc ggcgttagct
     8521 gtcacttgat tactatgttt caccccgccc ggtatccaaa ccgcatattg tggcggcacc
     8581 atccacaaag cattttctac cgtacaggta attgcaccat gtagtgccag tatcaactgc
     8641 cctttgcggt gctgatgtac aggactggtt aactcatccg ttccggcgtg aatacaaaat
     8701 gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat tgagattcaa cctgtgcatc
     8761 attttgtccg aatttagcga tattttgtca ttttagcttg attcaacata acaataaaaa
     8821 cggtaaggta cagcctcgtt tgtaacaatg agaagcatat gacctgttca acttcattaa
     8881 gcggtaaaaa caggattttc cttatcgctg gcattctgat gattgccaca acattacgcg
     8941 tcacctttac cggcgcagca ccgttactgg atacgattcg ttccgcttac tcgctaacga
     9001 cggcgcaaac cggcttattg accaccctgc cattattagc ctttgcgctg atctcaccgc
     9061 tggccgcccc ggtggcgcga cgttttggta tggaacgtag cctgtttgcc gcgttacttt
     9121 tgatctgtgc aggtatcgca attcgctctc tcccttcgcc ttacttatta tttggcggta
     9181 cagcggtcat tggcggtggg attgcattag gcaatgtctt actgccagga ttaattaaac
     9241 gcgatttccc tcattccgtc gccagactta ccggcgcata ttccctgaca atgggagctg
     9301 cggcggcact gggatcggcg atggtagtgc cgctggcttt gaacggtttt ggctggcaag
     9361 gcgcgttgct catgctgatg tgttttcctc tgctggctct ttttttatgg ctgccacagt
     9421 ggcgaagtca acaacatgca aatttgagta cctcacgcgc cttacatact cggggtatct
     9481 ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg gatcaactca ctggtctatt
     9541 acgtgattat tggctggctt ccggcgatcc tcatcagtca aggctatagc gaagcacagg
     9601 cgggttcact gcatggtttg ctgcaactag ccacagcagc tcccggtttg ctgatcccac
     9661 ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc ttgatgtgcg
     9721 cagtgggcgc ggttgggctc tgctttatgc cagcacacgc gatcacctgg actctgcttt
     9781 tcggttttgg ttccggcgca acaatgatac tggggttgac gttcattggt cttcgggcaa
     9841 gttctgcgca tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg
     9901 cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc taacggtgac tggtccgtac
     9961 cactgatggg tgttgcaata ctttcactac tgatggcgat tttcggactt tgcgccggga
    10021 gagacaaaga aattcgctaa tatccggtgc tatagtgacg tgacaaatca tgcgtgaaag
    10081 ggagaacaaa tacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg
    10141 atggttatcg cgtactggtc gaccgcctct ggccacgcgg tatcaaaaaa accgatttag
    10201 cccttgatga gtggaataaa gaaatcacgc cgtcaacgga gctgcgcaaa gcctttcacg
    10261 gcgaagtcgt cgattttgca acctttcgcg agcaatatct tgcagaactg gcgcaacacg
    10321 agcaagaagg aaagcggctg gcagacatcg ccaaaaaaca gccgctgacc ctgctctact
    10381 cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc agactggcta cgtagcttgt
    10441 gattttagta cagcatccgg cggttatttt tcaccagccg gatggtcccg tcgccacaat
    10501 gcccattcat caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct
    10561 gtctgcacag gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc
    10621 ataccaatct gcggcggttt aggtgcttct ggctgagaag gggttgaaca accagccagg
    10681 accagcaagc aaggcagaac aaaactgata attttcattt attgatctca catatttatc
    10741 caggcttaga gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc
    10801 aaaacatatt acgccttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt
    10861 aaataatcag catttacccg ccgtatcctg gagttgtttc gtgtcagatc agattatcgc
    10921 ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa agtctggttc gacagcttct
    10981 ggagatgctg gaaatggtca ctgatatgga gtcaacctac ctgaccaaag tggatgtcga
    11041 agcgcgcctg cagcatataa tgtttgcccg taatagccag aaaatgcaca tcccggagaa
    11101 ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc attgatgaaa actgtttttt
    11161 cagcgatgaa gtccccgacc gttggggcga ctgtattgcg gcacgcaatc ttggcatcac
    11221 cacatttctg agcacgccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc
    11281 cgccagcagt gagaagcgcc agtggagtga acgcgcggaa caggtcttac agttattcgc
    11341 cggactgatt gcacaatata ttcaaaaaga ggcactggtt gaacagctgc gcgaagctaa
    11401 tgctgcgctg attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc
    11461 gatttttgaa aatctgacga cgctgttttc tctcgcccgg catcttaacc ataagataat
    11521 gatcgcgttt atcgatctgg ataacttcaa attaatcaat gatcgttttg gtcataatag
    11581 tggcgatctg tttctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga
    11641 agttattggt cgtctcggcg gtgatgagtt tttggttgtt tcactgaaca acgagaatgc
    11701 ggatatttcg tcgctgcgag aacgtattca acaacaaata cgcggagaat atcacttagg
    11761 tgatgttgat ttgtattatc ctggtgccag tcttggcata gtagaagtcg atcctgaaac
    11821 gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg atgtatcagg agaaaaaaca
    11881 taaacagaaa acaccttttg tcacgcattc agcgctacat tcctgaggcg tattcacatc
    11941 cttttgattg gtgataacat gcgaatcggt attatttttc cggttgtaat ctttattaca
    12001 gcggtcgtat ttttagcatg gttttttatt ggcggctatg ctgccccggg agcataaaga
    12061 tgaaaaaaac aacgattatt atgatgggtg tggcgattat tgtcgtactc ggcactgagc
    12121 tgggatggtg gtaacgtcac atctaaaaaa tagcaaaggc tgcctgtgtg cagcctttgt
    12181 gcaatttacg cgttaacttt taatcttcct gtagataaat agcacaacaa tcgcaccaat
    12241 aacggcaacc acgaagctgc caaaattgaa gccatcgact ttaccaaagc caaacagcgt
    12301 gctgatccat ccgccgacta cggcaccgac tatccccagc aggatagtca taaagaatcc
    12361 accaccatct ttacctggca tgatccactt cgccagaata ccggcaataa gcccaaaaat
    12421 aatccatgac agaatgccca ttgtttcctc acttatctgt tttgcattag cgggttagtc
    12481 gctgataaaa agcatagcac aacatcggga ggggaagatt tgtgacgagc atcacggagg
    12541 aattttttgc gatggcgcag aaattgcgcc atcaacgatc agtgataatt accaaccaca
    12601 aacatcatgt tcgttttccg tgtcataaga acgcacggta ttcaccagat cttttatcac
    12661 ttcagccgcc acttctggca ccagcaaagt catcggcgtc tctgtttcat aatcgacaga
    12721 aacgccattg ctgttattgg tgacggtcac ggtatacgtt gctttgccca tgattcattt
    12781 cccgttatga atgactttcc gttgttgcgc gccttccatc aggacttcag gagcaacgaa
    12841 gaagtcaatg ttgaaataag tatcgtcagt catggcttca atgttgtgcc acttttctgg
    12901 agggaacacc gcaaactgcc ccgcttcgat aaggatcacc tgatcaggct ctgcactgtg
    12961 ttcatcagcg tagccgagat atttgaccgc cccatgcata acggaaaggc gtgggtaaac
    13021 ccccgggcgc gttcctttat caagatgacg ttcgaatatt ccggcaggcg cagtttgttt
    13081 attccagaaa ggcgttgagc gcgtatgaat ataattctgt gggatttgca gcatcctttt
    13141 ccctccttcg gtgaatgcgc tggaaacggt ttattccagc cgtttcaggg tacgcctgat
    13201 aatttgcatt ttaaatacca tttattggtt actttttagc accgtatcag agaagaatca
    13261 gggaggatta caggtgggaa gcccgcggca acgcgggctg aaagcatcag gattgcagcg
    13321 tcgccagtcg ggcggcgaaa cccacgaaca tcaaaccaat cagtgagttg cccactttag
    13381 ccagtttctt tttggtacgt atgtactgcg tgacaaaagc cccagaaata atcaggaagc
    13441 tcaaatagca gaaactcacc agttccagcg tcgtcgccag aataaagaat gaaattcccg
    13501 tatgtggggc attaacatcg ataaactgta cgaaaaacga cacatagaac aaaatggctt
    13561 tcggattagt caggctcaaa attaacgcgc gtttaaaaat ggcaccgtat tggggctcat
    13621 cggatttggt ctcgctattt ttacctttca gggtcgcgta gagaatttta ctccccagat
    13681 agagcaaata aaacgcacca agataacgta cgatgttgaa taatatcggg gtggtcttaa
    13741 ttaatgtcgc cactccagcc catgccagaa acatcaatac cgcatcgcca ataaatacac
    13801 cacaggccgc aagataaccg cctttcatac cgctactgac gctatttttg agtacaaaca
    13861 gggtatttgg ccctggcacc aacacaataa aaatggcccc aaccagatag gtccagtaat
    13921 tcagaacccc gtattcagcg aacacattaa cctctttaat tatctttcga tcatgcgcga
    13981 ttaaaggtga atatgctaac caatctgtag cggcttagaa aggagaaaat caggttttaa
    14041 ctcgatatca acccgataat tgaatcatta atcaggcatg ctccagtgaa aaattcgggt
    14101 agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat ccgcactttc gctgacgtcg
    14161 acagctttga aacataaacg gcccagacat tcgctggctg gtaatattcc ggtagcactt
    14221 gtactaaatg accactggcg ggaaacagcc tggcgacatc gatttttacg tggtccggaa
    14281 aaacaccgaa ggcgaatatt cctcgccagg cggtagagtg aatgaaggaa cagagaatga
    14341 agtcgtcatt caggaatcgg tatttactcg tcgtggtgtc gagcgcattt ggcgttaagc
    14401 tcaaataagt tca
//
LOCUS       DAOFSV010000117        14139 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.117, whole genome shotgun sequence.
ACCESSION   DAOFSV010000117 DAOFSV010000000
VERSION     DAOFSV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.117"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(30..1679)
                     /gene="pgi"
                     /locus_tag="QBH27_003555"
     CDS             complement(30..1679)
                     /gene="pgi"
                     /locus_tag="QBH27_003555"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDQ7015825.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2204..3553
                     /gene="lysC"
                     /locus_tag="QBH27_003556"
     CDS             2204..3553
                     /gene="lysC"
                     /locus_tag="QBH27_003556"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDQ7015826.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3806..4078
                     /locus_tag="QBH27_003557"
     CDS             3806..4078
                     /locus_tag="QBH27_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDQ7015827.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4079..4951)
                     /gene="rluF"
                     /locus_tag="QBH27_003558"
     CDS             complement(4079..4951)
                     /gene="rluF"
                     /locus_tag="QBH27_003558"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDQ7015828.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5299..6246
                     /locus_tag="QBH27_003559"
     CDS             5299..6246
                     /locus_tag="QBH27_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDQ7015829.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6316..7122
                     /locus_tag="QBH27_003560"
     CDS             6316..7122
                     /locus_tag="QBH27_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7015830.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7132..7539
                     /locus_tag="QBH27_003561"
     CDS             7132..7539
                     /locus_tag="QBH27_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDQ7015831.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7539..8033
                     /locus_tag="QBH27_003562"
     CDS             7539..8033
                     /locus_tag="QBH27_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDQ7015832.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8099..8896
                     /locus_tag="QBH27_003563"
     CDS             8099..8896
                     /locus_tag="QBH27_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDQ7015833.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            8907..9731
                     /locus_tag="QBH27_003564"
     CDS             8907..9731
                     /locus_tag="QBH27_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDQ7015834.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNVVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9783..11012
                     /gene="sorE"
                     /locus_tag="QBH27_003565"
     CDS             9783..11012
                     /gene="sorE"
                     /locus_tag="QBH27_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDQ7015835.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11394..12083
                     /gene="pepE"
                     /locus_tag="QBH27_003566"
     CDS             11394..12083
                     /gene="pepE"
                     /locus_tag="QBH27_003566"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDQ7015836.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIIDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12173..13804)
                     /locus_tag="QBH27_003567"
     CDS             complement(12173..13804)
                     /locus_tag="QBH27_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDQ7015837.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3424 a         3572 c         3702 g         3441 t
ORIGIN      
        1 gccttatccg gcctacatat cgacaatgat taaccgcgcc acgctttata gcggttaatc
       61 agaccattgg tcgagctatc gtggctgctg atttctttgt catctttcag ctctggcaga
      121 atacggttcg ccagttgttt acccagctcc acgccccatt ggtcgaaggt gaagatgttc
      181 aggatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc ccccaggctg
      241 aacggagtga tttcacgcag caggatggag ttggtcgggc ggttaccttc gaacactttg
      301 aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcgcgata ttcctgctca
      361 accacttcgc gggatttacc aaacgccagc gcttcggtct gagcaaagaa gttagacagc
      421 agtttctggt gatgatcaga gagcgggtta tgggtgatag ccggagcgat gaaatcgcac
      481 ggtaccattt tggttccctg gtggatcagc tgatagaacg cgtgctgacc gttcgtgcca
      541 ggttcacccc agataatcgg gccagtctgg taatccacaa ccttaccgtt acggtcaaca
      601 tacttaccgt tggattccat attgccctgc tggaagtacg ccgcgaaacg gtgcatatac
      661 tggtcatacg gcagaatcgc ttcagtttcc gcgccaaaga aattgttgta ccagatgcca
      721 atcagcgcca gcagtacagg caggtttttc tcggcaggcg tggtggagaa atgcttgtcc
      781 atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga gagaacaatc
      841 gacaggccaa tcgctgacca caaagaataa cgaccgccga cccagtccca gaattcgaac
      901 atgttggcag tatcaatacc aaactcgcca acggctttgg cgttggtgga aagcgccgca
      961 aagtgtttcg caacgtgctt ctcatcacct gccgctttca ggaaccagtc acgcgcgcta
     1021 tgggcgttgg tcatggtttc ctgagtggtg aaggttttag atgctaccag gaacagcgtg
     1081 gtttccgggt ttactttttt cagcacttcc gcgatgtgag tcccatcgac gttagaaaca
     1141 aagtgcatgt tcaggtggtt tttgtatgga cgcagtgctt cagtcaccat gtacgggccg
     1201 aggtcagaac cgccgatccc gatgttcact acgtcagtga ttgccttgcc ggtatagcct
     1261 ttccactcac cggaaataat cgcttccgag aaggttttca tcttctccag caccgcgttg
     1321 acttccggca taacatcttt gccatcaacc agaatcgggg tattgctacg gttacgcagc
     1381 gctacgtgca gtacggcgcg gttttcagtg cggttgatct tctcgccaga gaacatcgac
     1441 ttaatcgcgc ccgccagatc gcactctttc gccagatctt gtaatttcgc cagcgtctct
     1501 tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgtcgaaggt tgcggagaac
     1561 ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc tttcatttca
     1621 tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat gtttttcatt
     1681 agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc cagtcacaga
     1741 aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt agcgcagcga
     1801 agcatttatt agctgaacta ctgaccgcca ggtgtggatg aaaaatccgc atgaccccac
     1861 cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc tagcgcaggc
     1921 cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg ataacacctg
     1981 agggggtgca tcgccgaggt gattgaacgg ctggctacgt tcatcatcgg ctacaggggc
     2041 tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa gtggtggagc
     2101 acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt ccgctcttcc
     2161 cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg aaattgttgt
     2221 ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca gcgctgatat
     2281 tgtgctttct gatgccaacg tacgtttagt tgtcctctcg gcttctgctg gtatcactaa
     2341 tctactggtc gctttagctg aaggactgga acctggcgag cgattcgaaa aactcgacgc
     2401 aatccgcaat atccagtttg ccattctgga acgtctgcgt tacccgaacg ttatccgtga
     2461 agagattgaa cgcctgctgg agaacattac cgttctggca gaagcggcgg cgctggcaac
     2521 gtctccggcc ctgacagatg aactggtcag ccacggcgag ctgatgtcga ccctgctgtt
     2581 tgtcgagatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac gtaaagtgat
     2641 gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg cggaactggc
     2701 cgcgctgcag ctgctcccac gcctcaatga aggcttagtg atcacccagg gatttatcgg
     2761 cagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt atacggcagc
     2821 cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg tcccgggcat
     2881 ctacaccacc gatccacgcg tggtttctgc agcaaaacgc attgatgaaa tcgcgtttgc
     2941 cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa cgttgctacc
     3001 cgcagtacgc agcgatatcc cggtctttgt cggctccagc aaagacccac gcgcaggtgg
     3061 tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg cgcttcgtcg
     3121 caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg gtttcctcgc
     3181 ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca ccacatcaga
     3241 agtgagcgtg gcattaaccc ttgataccac cggttcaacc tccactggcg atacgttgct
     3301 gacgcaatcc ctgctgatgg agctttccgc actgtgccgg gtggaagtgg aagagggtct
     3361 ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg gcaaagaggt
     3421 attcggcgta ctggaaccgt tcaacattcg catgatttgt tacggcgcat ccagccataa
     3481 cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg gtgcagaagc tacattttaa
     3541 tttgtttgag taaacacagt atggcccgga agacattatt tcgggccgta ttgtttttct
     3601 tgtcattatg cccataaata aacgagcctg tactctgtta accagcgtct ttatcggaga
     3661 ataattgcct ttaatttttt atccgcatct ctaactaatt atcgaaagag ataaatagtt
     3721 aagagaaggc aaaatgaata ttatcagttc tgctcgcaaa cgaattcccg tacactgaac
     3781 tctctggctc actgagggca ccatcatggc actcccccgt attacccaaa aagagatgac
     3841 tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg gcgcggattg cgcatggtcg
     3901 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
     3961 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
     4021 ggatcctgag gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctgatt
     4081 aacgcccctt ctttttgcgc cccggcgagg taaaacgctt accgttggac gccgggcgac
     4141 cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgcct accgttttcg
     4201 gtttggcctt cgctttaggt tttgcctcgg aagaggaatt ttcaatgagc ttaaagagat
     4261 cgattaactc atcgtcggtt aaatcgcgcc actcacccag cggaatgccg cttaagctga
     4321 cgttcataat gcgcgtacgt tccagctttt tcacttcgta accaaagtgc tcgcacatgc
     4381 gacgaatctg acggttcagc ccctgcacca gggtaatgcg gaagacaaac ggtgcttctt
     4441 ttttgacttt gcacttttta gttaccgtcc cgaggatcgg cactcccgcc cccataccac
     4501 gaataaattc atcggtaatc ggtttatcaa ccgtcaccag atactctttc tcatgatcgt
     4561 tacccgcacg gaggatctta ttcactagat cgccgtgatt ggtgaggaaa atcagcccct
     4621 gggaatcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta acgaaatcga
     4681 caatgttatc gcgctcgcca tcttcggtgg tgctgacaat gcctacgggc ttgttcaggg
     4741 cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt actttcacaa
     4801 cgtcgccgga tttcacctga tcgccaatgg tggctcgctt gccattaagg aacacattgc
     4861 cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt
     4921 tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc ggaatatacc
     4981 gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa tgtgatctgc
     5041 cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct ggcaaaataa
     5101 ggataatcaa caaattacga atcgctgcac acctgtgctg atgactacta cgcagagaaa
     5161 caaatgtgca agagaaagcg ctatttcccc ccatgcaaag agtgaaaaat cgctttgaaa
     5221 ggccatcatg atggggttat atcagatcaa gcggtgagaa agcgtatttg cacaaatgtg
     5281 cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt gaagattgcc
     5341 caactctatt acgaacagga tatgacgcag gcgcaaattg cgcgcgaact gggtatttac
     5401 cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt caccatcgcc
     5461 atcaactatg actacaacga aaatctctgg ctggagcagc aagtgaagca aaagtttggc
     5521 ctgaaagacg ttgtggtggt gtcaggaaat gatgaggatg aagatactca actggcgatg
     5581 atggggttac acggcgcgca actgctggat cgcttgctgg aacctggtga tattgttggt
     5641 ttttcctggg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaggc ggggcaatcg
     5701 cggcagttaa tttgcgtgcc gattattggc ggcccgtccg gtaaactcga aagccgctat
     5761 cacgtaaaca cattaaccta cagcgcggca gcgaagctga aaggggaatc gcatctcgcg
     5821 gattttccgg ctctgctgga taacccatta attcgtaatg ggatcatgca gtctcagcac
     5881 tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg aattggctca
     5941 ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga gagtgacgac
     6001 ctgaatgccc gccaggttgc tggcgatatt tgctcgcgct tttttgatat tcacggcgca
     6061 atggttgaaa cgaatatgag cgaaaaaaca ctctctatcg aaatgaataa attaaagcag
     6121 gcacggtatt ccattggcat tgccatgagc gaagaaaaat acagcggaat tgttggtgca
     6181 ctgcgtggaa aatatattaa ttgtctggta acgaatagca gcacagctga actattactg
     6241 aaataaaaat cttcccgatg tttaccgtaa taaaaattac ggcgggtcct tattatctaa
     6301 aatcaggagt ctgttatgca aacgtggtta aatttgcagg ataaaatcat tattgtcacc
     6361 ggtggcgcat ccggcattgg tctggcgatt gtggatgaat tattagcaca aggcgcgaat
     6421 gtacagatgg tcgatattca cggtggcgat ggtcagtatg aaagccataa aggttatcag
     6481 ttctggccga ccgatatttc cagcgccaaa gaggtaaatc atacggtagc agaaattatc
     6541 cagcgttttg gtcgcatcga cggtctggtt aacaacgccg gggtcaattt cccgcgtctg
     6601 ctggtcgatg agaaagcgcc tgccgggcag tatgaactca acgaagctgc attcgaaaaa
     6661 atggtcaata tcaaccagaa aggcgttttt ctgatgtcgc aggcggtggc gcgacagatg
     6721 gtcaaacaac atgatggcgt gattgtgaat gtttcctcag aaagtgggct ggaaggctca
     6781 gaaggccaaa gctgttacgc cgcgaccaaa gccgcgctca atagcttcac gcgctcctgg
     6841 tcgaaagagc tgggtaagca cggtatccgt gtggtcggta tcgcgccggg gattctggaa
     6901 aaaacagggc tgcgcacgcc ggaatatgaa gaggcgctgg cgtggacgcg caatatcacc
     6961 gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc ctattgggcg cgccggaaga
     7021 ttagcagaag tggctgattt tgtttgttat ctgctgtctg aacgcgccag ctatatcacc
     7081 ggagtaacca ctaacattgc gggcggcaaa acgcgcggct aaggaggctt tatggtcaat
     7141 gccatttttt gtgcgcatgg caaattagct tgcgccatgc tggaatcggt tcagatggtt
     7201 tacggcgatg ccagggtcga ggcagttgaa tttgtgcccg gcgagaacgc cggagacatc
     7261 gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag atgagtggct gattgccgtg
     7321 gatttacagt gcggcagccc gtggaatgcg gcagcgatgc ttgcgatgcg caatcctcgt
     7381 ctgcgggtaa tcagcggcct ttctctgccg ctagcgctcg aactggttga taaccaggac
     7441 agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa tcgcaaagca aacctgcgtc
     7501 gtctggagac aggtggcaac agcggaggag gatttctgat gaacattacg ctcgcccgca
     7561 ttgatgaccg cctgatccac ggccaggtca ctaccgtctg gtctaaagtg gctaacgccc
     7621 agcgcattat tatctgcaat gacgaagttt ataacgatga agttcgccgg acattattgc
     7681 gtcaggctgc gccaccggga atgaaggtta acgtggtcaa tattgaaaaa gccgtcgcgg
     7741 tgtatcacaa cccgcaatat caggacgaaa ccgtttttta tcttttcact cgcccacagg
     7801 atgccttagc aatggttcgc cagggggtaa aaataggtac cctgaatatt ggcggcatgg
     7861 cctggcgtcc tggtaaaaaa cagttaacca aagcagtttc attagatgac gatgatatta
     7921 acgcatttca tgagttaaat aatcttggcg tcatactgga tttacgcgtc gtagcgtcag
     7981 atccctcaat caatattatc gacaaaataa atgaacagtt aatcgcgaat taaataatag
     8041 cgcctgtgta catatgcttt aacaggcagg gatgacgaca cttaaaaggt gacaaatcat
     8101 ggaaataagt actctacaaa tcattgctat atttcttttt tcctgtattg ccggaatggg
     8161 cagcgtgctg gatgaatttc agactcatcg tccgttaatt gcctgtacgg tgattggttt
     8221 aattctcggt gatttaaaaa ccggaattat gctcggtggt acgctggaat tgatagctct
     8281 cggctggatg aacgtcggcg cggcgcaatc tccggattct gcgctcgcca gcataatctc
     8341 cgccattctg gttatcgttg gtcagcaaag catcgccacc ggaatcgcca tcgcgttgcc
     8401 tgtggctgcg gcaggccagg tgctgacggt gtttgcccgt accatcaccg tagtgttcca
     8461 gcacgcggcg gataaagcag cggaagaagc acggtttcgc actctcgata ttctgcatgt
     8521 ctccgcgctt ggcgtgcagg cactgcgcgt tgctattccg gcactgattg tctcgctgtt
     8581 cgtcagcgct gatatggtga gcaatatgct gagcgccatt cccgaatttg tgacccgtgg
     8641 actgcagatt gctggcggtt ttatcgtggt ggtcggttac gccatggtgc ttcgcatgat
     8701 gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc ctcgcaggtg gctacctcga
     8761 tctcagtctg ctggcgttcg gtggcgtcgg cgtgatcatg gccctgctct acatccagtt
     8821 aaatccacag tggcgtaaag ctgaaccaca tccccagacc accactatca ccgcccttga
     8881 ccaacttgat gattaacgga gccatcatgg aacagagaaa aattacacgc agcgatctgg
     8941 tgagcatgtt tctgcgctcc aacctgcaac aggcatcctt taactttgaa cgtattcacg
     9001 ggctgggttt ttgctacgac atgatccccg ccatcaaacg actttaccca ttaaaagagg
     9061 atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa taccacgcca gccgtatgtg
     9121 gcccggtcat cggcgtcact gccgccatgg aagaggcgcg agccaacggc gcggaaattg
     9181 atgacggtac cattaacggc atcaaagtcg gtctgatggg accattggca ggagttggcg
     9241 atccactggt ctggggaacg ctgcgcccga ttaccgccgc gctcggcgca tctctggcac
     9301 tttcgggcaa cattctcggc cccctgctgt tcttctttat tttcaacgtg gtgcgcctgg
     9361 cgatgaagtg gtatggccta cagctcggct ttcgcaaagg ggtgaatatc gtcagcgata
     9421 tgggcggtaa tttgctgcaa aaactcaccg aaggcgcgtc gattctcggg ctgtttgtga
     9481 tgggcgtgct ggtgaccaaa tggacgtcaa tcaacgtacc gttggtggtt tcacaaacgc
     9541 ctgccgccga tggtgccacc gtcaccataa ccgtgcagaa catcctcgac caactttgcc
     9601 ctggtttgct ggcgctcggt ctgacgctgc tgatggttcg tctgctcaac aaaaaaatta
     9661 acccggtatg gctgattttc gccctgtttg gtttagggat tatcggtaat gcgctgggct
     9721 tcctgtcctg attcttcgcc ccggcgcaac cgtcggggcc atcgttcaac acgaggtggt
     9781 ttatgaaaac aacagctctg cgtctttatg gtaaacgtga tttacgcctg gaaacctttg
     9841 accttcctga aatgcaggag gatgaaatcc tcgcaacggt ggtcactgac agcctgtgcc
     9901 tctcttcctg gaaagaggcc aatctgggtg aaaatcataa aaaagtaccc gacgatgtgg
     9961 cgaccaatcc catcatcatc ggccacgaat tttgcggcga tattctggcc gtgggtaaaa
    10021 agtggcagca caaattccag ccgggtcagc gttatgtgat tcaggccaac ctgcaactcc
    10081 ccgaccgccc ggattgcccc ggctactcct tcccgtgggt aggcggcgaa gcgacgcatg
    10141 tggttattcc caacgaggtc atggaacaag attgcctgct ggcatacgac ggcgaaacct
    10201 attttgaagg ctcgctggtt gaaccgcttt cctgcgtgat tggcgcgttc aacgccaact
    10261 atcatcttca ggaaggtagt tataaccaca cgatggggat tcgcccgcaa gggcgcacgc
    10321 tgatcctcgg cggcaccgga ccaatgggac tgttggcgat tgattatgcg ctacatggac
    10381 ccgttaaccc ttcgctgctc gtcattaccg ataccgacaa cgacaaattg agttatgcgc
    10441 gcaagcacta tccatcagaa ccgcaaacac tgattcatta tctcaatgcc gccgatgcag
    10501 catttgatac gttaatggca ctgagtggcg gtcacggctt cgatgatatt ttcgtctttg
    10561 tgcctaatga aggactggtg actctcgcct cttccttgct ggcgacagat ggttgtctga
    10621 atttcttcgc cggaccgcag gataagcact tcagcgcgcc aattaatttc tacgatgtgc
    10681 attatgcatt tacccactac gtgggcacgt caggcggcaa taccgacgac atgcgcgcag
    10741 cggtcaaatt aattgaagag aaaaaagtgc aggccgcaaa agtggtaaca catattcttg
    10801 ggctgaatgc cgcgggcgaa accacgcttg aattgcctgc cgtcggcggc ggcaaaaagc
    10861 tggtgtatac cgggaaatac ctgccgctga cgtcactcac gcagattcag gatcaagcac
    10921 tggcggcgat tctggcgcgt catcagggga tctggtccgg tgaggcggag caatacctgc
    10981 tcgcccatgc agaggcaatt tcccatgatt aatcgcgcta cttaactctg catgtcgctg
    11041 gctggtcgcc gcggcaactt cgcgacctga tgtgcggctg attgctgaag cggcacaatg
    11101 tgcacgagaa ggataaaaaa acatcagcgc cttaaggggc gctcaaattg gtggcaaagt
    11161 gcacattgtt tatgccggat gcggaatgaa gccttattcg gcctacataa tcttgaaaat
    11221 tcaatatatt gcagttatct ggtaggccag gtaagcgtag cgcatcaggc aatcctgtgt
    11281 ttgtcatcag tctcagcgcc tgtgcaatat gtgacatcgt gagccatgtc agtaagtggt
    11341 taaattcgca gtggtagcat tccttccgtg ttttcttact aaccggagaa gtaatggaac
    11401 tgcttttatt gagtaactcg acgctgccgg gtaaagcctg gctggaacac gcgctgccgc
    11461 tgattgctga acagttgcag ggtcgccgtt ctgcggtatt tattcctttc gctggcgtaa
    11521 cgcagacctg ggatgattac acagcgaaaa cggctgcggt tctcgctccg ctgggtgttt
    11581 ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc gattgaaaat gcggaaattg
    11641 tgattgtcgg cggcggtaat actttccagt tgctgaaaca gtgccgcgag cgcgggctgc
    11701 tggcacccat tactgacgtg gtgaaacgtg gcgcgctgta tattggctgg agcgcaggcg
    11761 ctaaccttgc ttgcccaacc attcgtacca ccaacgatat gccgattatc gatccgcaag
    11821 gtttcgatgc gctaaatctg ttcccgctac aaatcaaccc gcacttcacc aacgcgctgc
    11881 cggaaggcca taaaggtgaa acccgtgagc agcgtattcg cgaactgctg gtcgtcgcgc
    11941 cagaactgac gattattggt ctgccggaag gtaactggat cacagtaagt aaaggtcacg
    12001 ctacgctggg tggcccgaac accacttatg tatttaaggc tggtgaagaa gcggtgccgc
    12061 tggaagcggg tcaccgtttt taagtgaatt gccggatgca tgatccggca tcttgttcat
    12121 agtgcctgat gcaacgcttt acgcgtctta tcaggccttc agggtcacgg tattaatact
    12181 cgtcccgtcc ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca
    12241 gcgagttcag acgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatac
    12301 tctgcacgtt ttgctggtgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
    12361 tgcgaaaacg atgtttgctg cggcgcaaac gacgggcgct ggtaacatcg ccagagaaga
    12421 aaaccgacat tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct
    12481 tcaagccatc aagcgaaaat gctcgccttg ctgccagcga tttgtcggca atttcgctgc
    12541 ccatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
    12601 cccagcggcg cgactcttct tctgccagtt cctcttttgg catccgcgcc agatacagct
    12661 taatggcggt atagagaacg ttgatgtcat cagccagctt acgcagctct ttctcctgcc
    12721 gtggctcgcc gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa
    12781 tgcgcagggt ttcgcgggca gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga
    12841 catccagatg tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
    12901 tgcaaaaccg tgccatcggg tcaacgaatg gcaacatgac caggcagcgt acaaggttgt
    12961 agaagacgtg gaaatagatc accaattccg cttttggcaa tggcaacttc cccattgtct
    13021 ctgccagcaa atgaacaaac ggcaggataa tcaggctacc caccagctta aacagcaggc
    13081 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
    13141 ggccggaacc gaggtttgca ccaatcacca gacaaagcgc cactgggaaa gagataatgc
    13201 ctgcggcggt cagagtcgcg gtcagcagta ccgcggcaag gctggagtag ctgataatgg
    13261 cgaacatcgc accaatcagc gcatccagca aaatgtcgcc agtcagcgag gcaaagatca
    13321 cctgaacgcc gtttgcctga gtgatcggcg ttacggcctg cacaatcaac tccagcgcca
    13381 gtaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
    13441 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataaatcga
    13501 aggtgagaat acgcgccatc agcgccgtcc cgacatctgc acccagcaca atcaccagcg
    13561 caggcgcgag agcaaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat
    13621 tactgctctg caccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
    13681 cgacgctccg gctaaggaca gtacgcagac gcgcgccgaa gacgcgcatt acgcctgttc
    13741 gaacaatatg ggtcccccag accagcaggg cgacggcaga aagcaggtga agcaaagtta
    13801 gcacggaatc aggttctcct ttttgttatg cattttcctg ttaagatttt tctaatcacg
    13861 ttgtttttat ccaggcattt ctcttgtatg tactttcagt ataagagatt tatacaagaa
    13921 agcatttaaa aaggcattta tataaaaatc cacctgtatc tgttgctatt tctgtcacac
    13981 tttcccctgt cgtttacacc agataatcgt tgtcactttt gtgacctctg agaagataaa
    14041 caacgaaata tttaagacca gatcctgtga ataaaaataa ttaagtaaca acggtattac
    14101 ggagattgaa cagcttacgg tagcaaaacg cataaactc
//
LOCUS       DAOFSV010000118        14046 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.118, whole genome shotgun sequence.
ACCESSION   DAOFSV010000118 DAOFSV010000000
VERSION     DAOFSV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.118"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..98)
                     /locus_tag="QBH27_003568"
     CDS             complement(<1..98)
                     /locus_tag="QBH27_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDQ7015838.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNT"
     gene            187..297
                     /locus_tag="QBH27_003569"
                     /pseudo
     CDS             187..297
                     /locus_tag="QBH27_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            341..496
                     /locus_tag="QBH27_003570"
                     /pseudo
     CDS             341..496
                     /locus_tag="QBH27_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1623..2768
                     /locus_tag="QBH27_003571"
     CDS             1623..2768
                     /locus_tag="QBH27_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015839.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTKWLKKEPRASYF
                     FLFRLYTRIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPHPIPEMD
                     SSIVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAM
                     GFPSQPKIAGPREKPMRTVHPDNIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(3299..3556)
                     /locus_tag="QBH27_003572"
     CDS             complement(3299..3556)
                     /locus_tag="QBH27_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015840.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(3610..4377)
                     /locus_tag="QBH27_003573"
     CDS             complement(3610..4377)
                     /locus_tag="QBH27_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7015841.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPELLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(4374..5432)
                     /locus_tag="QBH27_003574"
     CDS             complement(4374..5432)
                     /locus_tag="QBH27_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDQ7015842.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(5451..6440)
                     /locus_tag="QBH27_003575"
     CDS             complement(5451..6440)
                     /locus_tag="QBH27_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ7015843.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(6451..8616)
                     /locus_tag="QBH27_003576"
     CDS             complement(6451..8616)
                     /locus_tag="QBH27_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ7015844.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVHDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSICHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            9045..9479
                     /locus_tag="QBH27_003577"
     CDS             9045..9479
                     /locus_tag="QBH27_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015845.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            9697..12081
                     /locus_tag="QBH27_003578"
     CDS             9697..12081
                     /locus_tag="QBH27_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDQ7015846.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNEKRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDECQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSVGRLFVLVNKFDEKDRNGDGADAVCQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFLDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            12078..12983
                     /locus_tag="QBH27_003579"
     CDS             12078..12983
                     /locus_tag="QBH27_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDQ7015847.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAG"
     gene            12980..>14046
                     /locus_tag="QBH27_003580"
     CDS             12980..>14046
                     /locus_tag="QBH27_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDQ7015848.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPVTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARI"
BASE COUNT         3544 a         3636 c         3694 g         3172 t
ORIGIN      
        1 gtattgcggg agatccccag ttctctggca atcgcccggc tactcattcc ctgcttgtgc
       61 aggattttaa tttccataac tgtctcaaaa gtgaccataa gctctcctga atcaggagag
      121 cagattaccc cctggatctg atttcaggcg ttgggtgtgg atcactattg caccgttcgt
      181 gacacaatgg gccatacggg ccgacgttcc atgccgcgtt actacattca gcccggacgt
      241 aaaatgcgcc cgtcaaaaag aacggttcgc ttcgaaactc agccaggata ccacctctcc
      301 gccacacccg ggaagaagtt gatatggtgg ttgccggaca acagagctct tggattgact
      361 gctgcatcac tcaaccacgc tgaatatcaa aggagagcgt taccggttaa aagagaaacg
      421 taaagctgtg gtgatgaccc aaaacgcaac gcctgtcagt gaggatgaaa tggtgaaaag
      481 cggacagcat cagtaacgga agtatcttag cgggtacgaa aatggcagga tcgtgacatt
      541 gacagaatat ctcctgatca tcctgaatga ctgcagttgc tgtggaaaag taagctgtct
      601 ccacccacgg cacgacttaa caggcattcc gtaactcccc cagacttact atgacgcatt
      661 atcggtgcac ttaaaaccgc agaccgcatt gcgggtgtta ctgcatgaca gtctgaccgt
      721 attgtgagat gtcggcagga accgatgtcc gcgcaggggg gaacttcctg cagtacctga
      781 cgcttcaaat agtattaacc gtagggcgtt gttggttccg gatcgcggaa atgggagcac
      841 aggtctgcta cggggtgtac ctctgacagt aaatccggct cgctcatctg acagcctctc
      901 tcccggaaaa gaccaaaaat gagatcgtct cctcaacagc agagaagagg tcttgacaga
      961 caggctacat tgcatacgat ttatgaagaa aaagacagca aatatggagg ttatagcgga
     1021 gaacacccta cgaaattcct atgaaaaacg attgaaaaaa atatcaaatt cgattcgttt
     1081 ttatatgctt tttgggaacg tggcgatgac gaactgttgc atgtgcagag gagaatgcac
     1141 ccggaagcac cagaatcatc cagaaaggca gaacgattac gcatcaaacc aggagacagc
     1201 aatatcaagg taatacactg atttaaaatg aatttacagc aatacgtgaa aacatttttt
     1261 acttaaagga gacagatgcg ctgaagagag tgcaggtatt tttatataaa aatgagaaga
     1321 ttacgggata tatatccggc acactatgac cggagaatac cagtcaggct taaaaagaca
     1381 ataaatacag tgagttaaat acacagagaa aacgaaaacg tagtatcaga gggtacatct
     1441 tacaacagag actgaatttc cggtttttca taaccggcgc attccggaaa atgcaggcgg
     1501 cccggagctc ttctgacgcc agtacctgat agattgttct gtacattctg tacatcatgt
     1561 acggaggttt ttgtttgctt tcttaaaatc tgcaaatgtg ggaagagcag gtgaggtgta
     1621 ttgtgggaaa ggaactggat gatatctgca ccagctgtcc gtatatcgat gctgtaaaac
     1681 ggcacaaaca acagctcggt gccatagagg aatatacaaa gtggttaaaa aaagaaccac
     1741 gggcatctta tttttttctg ttccggctgt atacccgcat tcacaataca tttttccccc
     1801 aaaagcaaca actcccgttc actcccggtg gcactcactg cccggaacct gatgtcaccc
     1861 ttcgggactt aacgctttcc ccgggctacc acagtgatta tgcccctcac cccattccgg
     1921 agatggacag ttctattgtt gttcccccca ctaatgaaaa cacctcgcct ccggaagata
     1981 ctcccgacaa cactccggcc ggaggcaata ccggacaagc ggaaaagacc agaaacgcag
     2041 gactcacacc cattcctgaa aaacgttcag gaatgccccc ggaacatctg cggtttgcca
     2101 tgggttttcc atcgcaaccg aagattgccg gtcccagaga aaagccgatg cgcaccgtgc
     2161 atccggataa tatctaccgt gaaattatat ggttctgctc cagttacctg ctgaaatccg
     2221 gaccagaggc tacgaggacg attatcaact cagtattctc tgaatgggcc agcataaaca
     2281 atgattaccc ctcccccttt tcgtgggtgg acagcaggga cagtgaacag tgtgactggt
     2341 tatggaacgc catgcaggtc cggtgtgtgg gaaccccgct gaatcccctt accccggagc
     2401 agaaatactg gttcgcctgc gccacgtttg ataactggga aggctggaat gagcaacagg
     2461 tacagttttt actggaaagt aatcccagac ggaacagagc gaagtttacg caggcctcct
     2521 tccaggcccc caggattcag cataaagcga ttcttcttga tgagctgaaa agtgcccggg
     2581 agcaacaaaa aaggcgtgat gaacgtgctg atggttccgt cccgctgaaa ctgtccggaa
     2641 aaatccacaa acagcttgaa agtattgccc gaagtcgtgg tgtcctccca aaaaaactgc
     2701 tgaatgaaat gattgagcag gcgtaccagg actttgtggc aaatgagcaa cacaagacac
     2761 tgagttaagg accgcagggc ctccggttac cgattctgca gcatacacaa cgaatgtgtc
     2821 cggctcttac ctgtgggggc cgcactaaag ttacgcattg agtatgagtg ggtaatcccg
     2881 gatgagaata tcccagtttt tacactattc actgaaggtg cgacatatca ttcagtctct
     2941 ctgaattctt catttcagtg gccccggcta cagtcgttta atctcctcca cgaaccgggg
     3001 ccgcaacttg ttgtgtcaat accaaattgt tggtcattac tgtcttgtgt cactggcgct
     3061 gacaagtgaa ctgaaatccg caccttaata ttctgactgg ctgcgtacat tcatggcatg
     3121 aagcggccgg agcggctgaa ctgaaggtcc gttgtgagcg aacagcggac attcactaca
     3181 catggttcaa acatgaaccg gagctttatt gtctagctgt cagtgtactg cgatttaccc
     3241 gctcggctgt ccagaaaatc ggtctgaaat ccagtcatta aagggcctta gcctgacatt
     3301 aagcaacttg gtaggcggat agataataga aataggaatg cgcaggtctg tcatgttgcc
     3361 tagaacggtg acaagctgtc cggtgcgaaa tcattccggc agcgacaggc tcatgtgtca
     3421 gcccgacgcc cagaccgtca acgagcatgt ggatctgcga ggcgttatcg ttcgcagcga
     3481 tgttaaaacg aacaatctgc tcatcttcta gaagcacaag tgctttaatg acattaaggc
     3541 ggtctatatg ttgcattaca gacttcctat tgatctttac ctgaggctga agcaaccacg
     3601 ttaatccgac tatttttcga ttgcaccatc cactatcacc atcgatcttc cctgagcaca
     3661 acgttcaact ctcccgcgta cgccgtatac ctgcgcaagg ctctctgcat ggatcaccgt
     3721 ttgcgggtcg ccgctgtcga taagtttccc ctctttcagc atgatgacct gggcggcatg
     3781 ccgcagtgcg atattgatat cgtgtaagac gaccagcgta accatattgc gtcttcgcgt
     3841 ttcgcgcgat acaacatcca tcacatggaa ctgataattg agatccagcg cgctcagtgg
     3901 ttcatccagc aataataatt cggggcggcg aataagcgac tgtgcaagcc ccaccagctg
     3961 cttctgaccg ccggacaggc tatcgaggta gttcattgcc agatgtgcga tgcctagctc
     4021 ttcgagcagc gctatagcct gcgcctgatt ttgcccggcg ccggaggcgc gctgggcgac
     4081 aaccaccgac tccagcacct gtaaatgcac gccctgcggc agggactgcg gcaggaacac
     4141 aactttttca gcccgacgag caaacggcag cgataagaga ttctcttcat tcagccaggc
     4201 ttcgccactg gcgcgattta gcccggccag ggcgcgcagt agcgttgatt taccgcagcc
     4261 attaggcccc agcagtacag tgacttcgcc gcgcggcagc gtagaaatcg acagatgctc
     4321 aataatccgc cgtttgccgt aaccggcgca cagcgcatta atcgtaagcc cgctcatgac
     4381 atactccctc gatgacgcat tacaatgctc agaaagaacg gaacgcctac cagcgaggta
     4441 acaattccta ccggtaaaat gacaccggga atgatgttct tcgacgctac cgaggccagc
     4501 gatagcacca gcccaccgat aaggatgctt ccgggcaaat aaaagcgatg atcttctccc
     4561 agcaacaggc gggaaatatg cggtgcaacc aggccaataa agccaattgg ccccacaaac
     4621 gcaaccgaaa gggccgccag caggctgatg cgcagcaggg aacccaggcg caaacggcga
     4681 acatcaatgc caaaactcat cgcccgctct tcgccgaggc gtagcgcggt aagctgccag
     4741 gcacgacgca gcgaccaggg taaaacaatc gccatcgccg ccgccagcac cgccagtttc
     4801 tcccatgatg cgcgagccag gctgcccatc gtccagaaca ccaggccctg caaagtatct
     4861 tcatcggcga caaactgcat aatggacacc agcgcattga aggtaaacac cagcgcaatc
     4921 ccaaacagca caacgcccga cgtcggcacg cgcgtccagc gggtcagact atcgagcagc
     4981 agggcggaca ggagcgcaaa aagaaaagcg tttgccggta taaacccgct ttcgggaacg
     5041 ccgggaatac cgatgcccag aacaatcgcc agcgctgcgc caaaggccgc cgccgacgaa
     5101 acgccaagcg taaacgggct ggcgagggag ttattcagaa tagtttgcat ctctgccccg
     5161 gccaaaccca gcgccattcc taccaggagc gccatcagcg catagggcaa acggatgtcc
     5221 caaacgataa cccgcatgcc gttagtcgcc ccggacggat gcatcaaagt ctttatcagc
     5281 gaatgtaaag ggagccctga ggggccgagg gtgaaatcga gcagcagcga agcaatgata
     5341 gcgataaaaa taaccgccag tattaccaga cgctggcgaa aaagctggcg atagcggttc
     5401 tcgaccgaca gcgtactttc caatacagtc atggtttttc acagcctccg ttagcgcggc
     5461 ggattgagca catcgctcat ctgcgcatcg cttaacgtca ggccaaagaa ctgccgataa
     5521 aactcacgcg tctctttgcg tatatccaca tcagcaaatc gcgcaggata aagcgttttc
     5581 gccagccaca aaaattgcag cgcctcttcg ctggtttcac ggcaccacca gaacattcct
     5641 ttcggattaa cgtaaacgcg atgatgaatg accgcgtcca cgcttgccca ctgcggagaa
     5701 ctcagaatgg catccgcgtc gcgcttgttc atcgcgatga taaccgccgg attggcagtg
     5761 acaattttct ccagcgcgac ttcacccgaa cggttttgtt gatgctcgcc aaaccaattt
     5821 tcagcgatat ttttgccgcc ggccaggtca atccagtcct ggtttagcga gggtctaccg
     5881 gtggtggtca gcggattgcc catactgtga tagagcgaca cgcgctgtga cgcaggaaga
     5941 tctttcaggc gcccggtgac cagggccacg ttgcgatcga agtagcgttg ataggcgagc
     6001 gctttttgct gcgcatcggg ccccagcacc gctgccgttt gctgtacgcg ctgcgttaac
     6061 gcccgcagtg aattcgcctc aaacgccagc acctgcaccc cagcctgttt cagcaacgcc
     6121 tgttgaggca ctaccatatt ctgcggtaca aaaagcatat cgacccccag cgcgataatc
     6181 tgttcgggat tgatttcatg cccgctgttt acgctaactg ctgcgacttt ctctatgcgc
     6241 ggcaggctct ggcgaaacag ggggatcctt tttgcaatca tcgaggttcc gacaatgtta
     6301 tcgccgtacc ccagcatggc aataatggta ttttgcgccg gccacggtga cgcgatacgc
     6361 acctccttgt gcgtattttc tgcgtgcagg ctggccgcca gcagcagcgc cagcagcgga
     6421 aataaacggg agagtttcat catttatcca tcagaaatcg aaggtgacgg atgcacgcac
     6481 ttcgcggccg ccgccgataa acgcacttgg ggaaccgtta tcgccatcgg tacccgatgg
     6541 gaagatagaa gcccagtaat gtttatcaaa cacgttgttt accaccacgc ggaaagtggt
     6601 tgggacgttg ctcactttcg tggtgtaacg cgttcccaaa tcccatgtgg tatagctgct
     6661 ggcgtaagag gtgttggtat cgttcgccgc gcgtttgccc gtataatgga cgttagcgct
     6721 gtaaacccat tccggtatgg acggcaaact gtattccgcc aacagattgg cctgcacttt
     6781 cggcacgccg acaacctgtt tattgctggt tgaggcattc gccgtatctt tcagtttcgg
     6841 gtcgaggaag gtcacgccgc tgtaaatatt cagcccctgc cacacattcc cggcagcggt
     6901 taactcaagg ccgttgttaa cctggttacc ctgctcttta tacacgttat ccgtatcaag
     6961 ataggcaaac ggacgttcca gtcggaacag cgcggcgcct agattcatct cgccgatgtc
     7021 cgattttagc cccacttcat actgcttgct gcgatacggg tttagcgttt gaccggcatt
     7081 ttttacgctc tcatccgtcg gtgcggtacc gccctgctcc agcgaatcgg catagctaac
     7141 gtaggccatt gtgttagggg taattttata catcagcgcg gcgttcggac ttaaaccatt
     7201 ttcatcaacc tggttcgttt gattaccgtg cttatcgtag tttttagtct gcagccagct
     7261 ctggctgaga tagaacattg ccgaccactg cggcgtaaat gtcaccgtat cgccgagcgt
     7321 aacgctctgc tgagtattta cgctggagcg gtatcgcggc ccgccggtgc ggattttgcc
     7381 atcgccctgc tcatctatcg catccgggtg atacatattt gttgtacccc agctatagct
     7441 ggaacctgtt cctttggcac tataaagcga ccagacatat cccgtggtag aaagtgacag
     7501 atcgtggcag atagagccgg tatcaatatg accattcagc ccggcggtgt tgctcaggac
     7561 gcgaaaacgt ccggcagcgg tggaatcctt catcgaacga gagatatcgc cctgattgtt
     7621 gagtatttta ctggaaacac tacgcatcgc gcggtcagcc tgctgccagc ccacgccagc
     7681 gttcatggac cagtcgtcgt taaagtagtg gatcaggcga gtgctgatgg tatcggtagt
     7741 gaggtcgttg cccgcagtgc tgagcgccag atttttgtcc ttcggattcg gcgcagacgg
     7801 cagtttgacg ttcggcccat agttaaagct accgacatag cctttctgga taaattcgta
     7861 atggctggcg tcgagctgta gctgagtgcc cggctgaata ttccagtcga gcgcaacgga
     7921 aacgagtttg cgacgcagcg tgctgtcatg cacattgcct tccccttcct gatcaagcag
     7981 gttcacgcga tagccaaacc gtttgttttc atcaaaatgg ccacccagat cggcatggcc
     8041 ggtaaacgca ctgcggcttt gatagcccag cgtcacttta cgcagcgtct cttcggttgg
     8101 gcgcttcgcc acgaaattaa actgccctgc tgggctcgcc gggccgtaca gcgcgccggt
     8161 caaactgtta agcacatcca tgcgctcaag catttccacc ggaaacgcgg ttgttgaaac
     8221 gatattcagc ccgtccagac ggctgttggc caccacgctg ccctgcatcc cccgactttg
     8281 cggacgcccg acatccattc caccgcgcgc ctgcatttgc gtactgggag agtatttcag
     8341 catctcgctg acgctttgcg cctgctgatt ttcaatcatc tctttagtaa cagtggtgct
     8401 ggaatatggt gtttcgatcc aggatttcgt tcccagcggg ccgacatcta tatcgttcgt
     8461 tttgaaaccg gcaccggcgg cagaatcggt ggtgtcggcg gcggtgttgc ccgtgacaat
     8521 catttcatct tcagaagtag acgaggcatg agcatgaagc ggtatgaaag acgctaatgc
     8581 tggcaaaagc gcagcgaaag ttatttgagt tttcatgaat cactgatcgt tatgtaaata
     8641 tttatattac gatcggtgca taggcaaaat tatgatccag atcacacgtg aatgtaagtt
     8701 atttacaaat tacgctaacc ttctgtctcc gctctttgca gcataaaagt atggcggcgc
     8761 gttgttgaaa gcaggagcgg ccgggttgtc agaaagctgg tttggacgca agtaatatat
     8821 aacaactctg attaccgcat actgtcagga cagtatctac ttgagcgtcc gcctttggca
     8881 caaagcggac tgtcagatta gattcagacc tgtactcatt agctgataca aatcagccac
     8941 atatacctgc attcaaaata atttcagaaa gatattatct ccgtgaatcc cccgccacct
     9001 ttccggtgca cggggttttg tcttttttca ctgggaatac atgtatgaat ccgtctgatg
     9061 ccattgaggc aattgaaaaa ccgctctcct ccctgcctta cccgatttcc cgtcacatcc
     9121 tggaacatct gcgcaaactc acccgtcacg aacccgtgcc tggcattatg ggtaaaagcg
     9181 gggccggtaa atcctcactc tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg
     9241 cactcatgac agtgcttccc ggacatgccg ccatccctct gatgacacgc ctgcaggacg
     9301 aactgcgcac ggagtctgtc cgcactcaga cccgtgaaca gtttaccggt gcggtggacc
     9361 ggatatttga cacggcggag agtgtctgca ttgcctctgt tgcacgcacg gttctgcgcg
     9421 ccgtccgtga ctcggtggtc tctgttgccc gcgcggtatg gaactggatt ttcttctgaa
     9481 cccggtggtg atgtaccagg ctgttgttac agatttttaa ctccatgaaa acactatgca
     9541 aacgcagcca tacccggggt gtccccttgc gttgtgtttt tcaagttaat agaatacatc
     9601 gatcgttact atcatatcga tcgttataaa cgatcgtttg tggtaaatga cctcctccgt
     9661 ggttgtcatg aaaatgcgtc tcaggaaggt taaataatgc acgaaaagaa catcgccctg
     9721 ctttgtgatg aagccgaccg acttttgcaa ctgaacatta atctgctccg gcaaatggtt
     9781 gaggagccag atgtgttatc tgacagtaag aacgaaaaaa gactgctttt tgataaacag
     9841 aaagcactga aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc ccgcagggag
     9901 atggtgctgg ctgttgtcgg cacgatgaaa gcaggtaaat caaccaccat caacgccatt
     9961 gtggggcagg aaattctgcc taaccgtaac cgccccatga cctctgtacc gacgctcatc
    10021 cgccacgttc ccggaaaaac tgagccggtt ctccatctgg aacatattca gcctgtccgc
    10081 aatttattaa tcacactgca ggaaaaactc gccaccccgg caggacagca ggtcgcacag
    10141 accctgcagc aaaccgggga tacccgcgaa ctgctggata ttctgacgga tgatggctgg
    10201 ctcaaaaatg aataccacgg ggaggaggaa atctttaccg gactggcatc gttaaacgat
    10261 ctggttcgtc ttgctgcggc aatggggagt gaatttcctt ttgatgaata cgcagaagtg
    10321 cagaaactgc cggtgatcga cgtggaattc agccatctgg tggggatgga tgaatgccag
    10381 ggaacactca cactgctgga tacccccggc cctaatgaag ccggacaacc gcagatggaa
    10441 gtgatgatgc gggatcaact gcagaaagcc tctgcggtgc tggcggtgat ggactacacc
    10501 cagatgaact cgaaagccga tgaggaggtt cgtaaagagc ttaatgccat tgctgatgtg
    10561 tctgtcggcc gcctgtttgt gctggtcaat aaatttgatg aaaaagaccg taatggcgat
    10621 ggggctgatg cagtctgtca gaaggtaccg gcaatgctga acagcgatgt gctgcccgcc
    10681 tcccgcgttt atcccggatc ctcacgccag gcatacctgg ctaaccgtgc gcttcatgag
    10741 ttacggaaaa acggaaccct tcctgttgat gaagcctggg tcgatgattt tgtcagggaa
    10801 gccttcggtc gcatgaaaaa agattacgtc tgtaaagaca gcgaactggc aactgaaggg
    10861 gcgacagacc tgtgggaagg ctcacttatc gatcaactga taacggaagt catactgagt
    10921 tcacactcca gagctgcggc actggcggtt gattctgccg ccgcaaaact gatgcagaat
    10981 gcagaaaata tcagtgaata cctgtcgtta cgccatcagg ggctgatgca aagtattcag
    11041 tcactgcagg cgcatatcac cagcctgctt gaggatatcc gggaaatagc agactgtcag
    11101 gagcaggtga ccgctgatgt cagaatggcc atggaggaga tcgatgccag aacccgggaa
    11161 ttactgacgg gggtctgcac ctcactggaa gaggagctga atgactattt cagaagcggt
    11221 aaacgcaaag aacagcaaat gctggaggaa gaaaattcag cgcagccccg ggagagaaac
    11281 gcattcgctt tctttctcga tattttcgga accggaaatc agcatgacag gatgcgggac
    11341 tttgatcccg acagccccga aataaaattc agtgatcgca gggaagccct tgaactgatg
    11401 acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg ctgaagcaca gttccggcct
    11461 gagctggaga aaatcgtcag cgggattgaa acaggttttc gtggcacggc cctgtacgcc
    11521 acagaaaaca ttgccggtcg catcaatacc cgcctggagg atgagggctt taccgtaaaa
    11581 atcagttttc cggcagtcag tcagttacaa acccggctcg cggtaaaaac aaatctgagt
    11641 gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc gtcggcagag tggtttatgg
    11701 ggaaagattt gtggagcgtt tggcaccagt gactggggct gggaaaccta taaagaggat
    11761 gtgagccgca gtgttatcaa tatcaacacc gtcaggaagg aagttatgtc actgacccgg
    11821 gcatatttcg gggagctgca ggcatccatt gagcaggata ttaaccagcc cgtccgccag
    11881 gaaatcgatg cctttttctg cgcattcagg gagaaagttg aacaactgcg taacacgctg
    11941 attcagagct ctgaagatca taaacgcgat cagcaggcac aggaacggct taccaggaga
    12001 cttcaggcat taaatgaaag ggttcctgag ctcattactg acagtaaggc gctgagggaa
    12061 gagctggaga caatgctgtg acttcaccat ttattcaaca aattgctgat aaccgggtat
    12121 gtcaggtact cacctgtctt cctgaaaaat ttgtggttga tttcgcaaac ggaatcgatg
    12181 ttgcacagga gcatatccgc acggccggag agcgcacgtt cttccggcgt ttaaaagagg
    12241 gactgaccgg cgaaggcgct gcacggcaga atgccatcaa tgcttcggtt gcacagggcc
    12301 ttgaggcgtc cctgcgctgg ctgacggagc tgacaacgtc acttgccacc acaaactacg
    12361 cgattacccg ggtaaacgac agggtcagtt cactggtcag tgatactgcc aggctggcgc
    12421 attattccgc agatacacgg gagcagttac tcatcctggc cgatcaggtt caccataaac
    12481 tgaatcatct tgaagaaaaa ctccaccgtg ttgaccaggt tcagcgggcg cagttacatc
    12541 ttgagcagat attctcatgg tggagcgccg ggcgatatgc gtcattttcc cctgccggac
    12601 gttgttatgt ggcgcttgaa gagcttcgct ggggcgcgtt tggtgatgtg atacgtcagg
    12661 gcgaaacagg ccaggttaac cagctactgg atattctcag acataaagca ttaacgcaga
    12721 tggcacagga gagtggcggt agtgcaaccg tgcgtctgaa cactctggac tggcttggtg
    12781 gtcagggccg ggagcaggca gataacgagt ggcatgacgc gattaactgg ctgggagact
    12841 ggtgcagtga agagcagcat ccggtgatct ggtcaaccac gcaggctgca gaacacttac
    12901 cggttcgcat gcctcgcctc tgctctgcag aacgcctctc tgaaagtatg gtcgatgaaa
    12961 tatttcagaa gggggctgga tgagtacaga aatgaaaacg gggctggtgc tgtccggtgg
    13021 cggggcggtg ggcgcttatc aggcgggagt ggttaaggca ctggcagagt gtggtacaca
    13081 gatcagcatg gtttcagggg ccagcattgg cgcattcaat ggtgccatta tcgcggcctc
    13141 tcccgatctg tcagaagctg ccgtacgcct ggaggcgctc tgggatcatc tggggaataa
    13201 tcaggtgctg tcggtaaacc gattggttta cttttcattg ctgaaaaaat tgttccaggc
    13261 aatgaacctc tgccagatcc ccggacgtgc aggagcactg cttacgacgc ttcttcgcca
    13321 tatatcgata ctcaacgggt ttgacaatct gatggctcag ccgttgttgt cagatgagcc
    13381 cctgacagcg ctgttggatc attatcttga tactgatgct ctggcagacg ggctaccgct
    13441 gtatgtgtcg ctgtacccca cagaaggggg catgcaggat attattgact gcattcgtgc
    13501 tgaactgggt gccggaacca cgaaaaacgc cgtttttcag catatccaga gcctgccccg
    13561 cggacagcag aaagaggctc tgcttgcgtc agccgcgctg cccctgctgt tccgtccccg
    13621 tgaggttcag gggacaatgt tcggtgatgg tggtatggga ggatggcgaa atatgcaggg
    13681 aaatacccct gtgacgcctc tggtcgatgc cggatgcaat atggtgattg tgacgcatct
    13741 gagtgacggt tctttatggg atcgccaggc ttttccggtc accacaatcc ttgagatccg
    13801 tccccggaaa aggctgaaat atgcaggtga tggtgacaac agcggcggtc tgctcagttt
    13861 cacgttggca cataccgacg cctggcgtca gcagggctat gaagacacga tgctgacgat
    13921 ggagcatatc cggaaaccgc tggcagcacg tcaggtactg tcccggtcag aaacggtgtt
    13981 gcagaaaagt ctggagataa cggaagaggc agatttggca ctgagaaacg cgatggcccg
    14041 gattaa
//
LOCUS       DAOFSV010000119        14044 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.119, whole genome shotgun sequence.
ACCESSION   DAOFSV010000119 DAOFSV010000000
VERSION     DAOFSV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.119"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            20..565
                     /locus_tag="QBH27_003581"
     CDS             20..565
                     /locus_tag="QBH27_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDQ7015849.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     DVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            540..2465
                     /locus_tag="QBH27_003582"
     CDS             540..2465
                     /locus_tag="QBH27_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDQ7015850.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHCMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLHVDEAINKTYT
                     RRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2462..2668
                     /locus_tag="QBH27_003583"
     CDS             2462..2668
                     /locus_tag="QBH27_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDQ7015851.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2665..4266
                     /locus_tag="QBH27_003584"
     CDS             2665..4266
                     /locus_tag="QBH27_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ7015852.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4247..5566
                     /locus_tag="QBH27_003585"
     CDS             4247..5566
                     /locus_tag="QBH27_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDQ7015853.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAVAPQSAQARSDTALDRLMQGAPAPLAAGNLASDAVNDLLNTPV"
     gene            5576..5908
                     /locus_tag="QBH27_003586"
     CDS             5576..5908
                     /locus_tag="QBH27_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDQ7015854.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            5964..6989
                     /locus_tag="QBH27_003587"
     CDS             5964..6989
                     /locus_tag="QBH27_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDQ7015855.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLV
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQLEGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7031..7429
                     /locus_tag="QBH27_003588"
     CDS             7031..7429
                     /locus_tag="QBH27_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDQ7015856.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVTLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7441..7794
                     /locus_tag="QBH27_003589"
     CDS             7441..7794
                     /locus_tag="QBH27_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDQ7015857.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISTDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7806..8384
                     /locus_tag="QBH27_003590"
     CDS             7806..8384
                     /locus_tag="QBH27_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015858.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGDSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8381..8776
                     /locus_tag="QBH27_003591"
     CDS             8381..8776
                     /locus_tag="QBH27_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015859.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8784..9524
                     /locus_tag="QBH27_003592"
     CDS             8784..9524
                     /locus_tag="QBH27_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015860.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELSAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            9540..9962
                     /locus_tag="QBH27_003593"
     CDS             9540..9962
                     /locus_tag="QBH27_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDQ7015861.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLALMKRQAEPAGSDSN
                     RQVTVEDVIRTGAFLVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQAEDAGPAEPVSAGKCSTVS"
     gene            9944..10378
                     /locus_tag="QBH27_003594"
     CDS             9944..10378
                     /locus_tag="QBH27_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDQ7015862.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10371..12932
                     /locus_tag="QBH27_003595"
     CDS             10371..12932
                     /locus_tag="QBH27_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ7015863.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYAARQEELNKALKDGKILQADYNTLMAAAKKDYETT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSVQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            12929..13258
                     /locus_tag="QBH27_003596"
     CDS             12929..13258
                     /locus_tag="QBH27_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015864.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWQQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13258..13956
                     /locus_tag="QBH27_003597"
     CDS             13258..13956
                     /locus_tag="QBH27_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDQ7015865.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13961..>14044
                     /locus_tag="QBH27_003598"
     CDS             13961..>14044
                     /locus_tag="QBH27_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7015866.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTP"
BASE COUNT         3212 a         3579 c         4433 g         2820 t
ORIGIN      
        1 tttgtccgtg gaatgaacaa tggaagtcaa caaaaagcag ctggctgaca ttttcggcgc
       61 gagtatccgt actattcaga actggcagga gcagggaatg cccgttctgc gaggcggtgg
      121 gaagggtaat gaggtgcttt atgactctgc cgacgtcata aaatggtatg ccgaaaggga
      181 tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa gaactgcgac aggccagcga
      241 gacagatctc cagccaggga ctattgagta cgaacgccat cgacttacgc gtgcgcaggc
      301 cgacgcacag gagctgaaaa atgccagaga ctccgctgaa gtggtggaaa ccgcattctg
      361 tactttcgtg ctgtcgcgga tcgcaggtga aattgccagt attctcgacg ggatccccct
      421 gtcggtgcag cggcgttttc cggaactgga aaaccgacat gttgatttcc tgaaacggga
      481 tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag
      541 tgaatatatc gaacagtcag gttaacaggc tgcggcattt tgtccgcgcc gggcttcgct
      601 cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc
      661 cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca
      721 tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg
      781 gttattccaa aatgctgctg ggtgtttatg cctactttat agagcacaag cagcgcaaca
      841 cccttatctg gttgccgacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc
      901 cgaccatccg cgatattccg tcgctgctgg cgctggctcc gtggtatggc aaaaagcacc
      961 gggataacac gctcactatg aagcgttttt ccaatggtcg tggcttctgg tgcctgggcg
     1021 gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt ggcgggttat gatgaacttg
     1081 ctgcctttga tgaggatatt gaacaggaag gctctccgac gttccttggc gacaaacgta
     1141 ttgaaggctc ggtctggcca aagtccatcc gtggctccac gcccaaagtg agaggcacct
     1201 gtcagattga gcgtgcagcc agtgaatccc cgcattgtat gcgttttcat gttgcctgcc
     1261 cgcactgcgg ggaggagcag taccttaaat ttggcgataa agagacgccg tttggcctca
     1321 aatggacgcc ggatgatccc tccagcgtgt tttatctctg cgaacataat gcctgcgtca
     1381 tccgccagca ggagctggac ttcactgatg cccgttatat ctgcgaaaag accgggatct
     1441 ggacccgtga tggcattctc tggttttcgt catccggtga agagattgag ccgccggaca
     1501 gcgtgacctt tcacatctgg acggcgtaca gcccgttcac cacctgggtg cagattgtca
     1561 aagactggat gaaaacgaaa ggagatacgg gaaaacgtaa aaccttcgtg aacaccacgc
     1621 tcggtgagac atgggaagcg aaaatcggtg aacgtccgga tgctgaagtg atggcagagc
     1681 ggaaagagca ttattcagcg cccgttcctg accgtgtggc ttacctgact gccggtatcg
     1741 actcccagct ggatcgctac gaaatgcgcg tatggggatg ggggccgggt gaggaaagct
     1801 ggctgattga ccggcagatt attatgggcc gccacgacga tgaacagacg ctgctgcatg
     1861 tggatgaggc catcaataaa acctataccc gccggaatgg tgcagaaatg tcggtatccc
     1921 gtatctgctg ggatattggc gggattgacc cgaccatcgt gtatgaacgc tcgaaaaaac
     1981 atgggctgtt ccgggtgatc cccattaaag gggcatccgt ctacggaaag ccggtggcca
     2041 gcatgccacg taagcgaaac aaaaacgggg tttaccttac ggaaatcggt acggataccg
     2101 cgaaagagca gatttataac cgcttcacac tgacgccgga aggggatgaa ccgcttcccg
     2161 gtgccgttca cttcccgaat aacccggata tttttgatct gaccgaagcg cagcagctga
     2221 ctgctgaaga gcaggtcgaa aaatgggtgg atggcaggaa aaaaatactg tgggacagca
     2281 aaaagcgacg caatgaggcg ctcgactgct tcgtttatgc gctggcggcg ctgcgcatca
     2341 gtatttcccg ctggcagctg gatctcagtg cactgctggc gagcctgcag gaagaggatg
     2401 gtgcagcaac caacaagaaa acactggcag attacgcccg tgccttatcc ggagaggatg
     2461 aatgacgcga caggaagaac ttgccgctgc ccgtgcggca ctgcatgacc tgatgacagg
     2521 taaacgggtg gcaacggtac agaaagacgg acggcgagtg gagtttacgg ccacttccgt
     2581 gtctgacctg aaaaaataca ttgcggagct ggaggtgcag accggcatga cacagcgacg
     2641 caggggacct gcaggatttt atgtatgaaa acgcccacca ttcccaccct tctgggaccg
     2701 gacggcatga catcgctgcg tgaatatgcc ggttatcacg gcggtggcag cggatttggt
     2761 gggcagttgc gggcgtggaa cccaccgggt gaaagtgtgg atgcagccct gctgcccaac
     2821 tttacccgtg gcaatgcccg cgcagacgat ctggtacgca ataacggcta tgctgccaac
     2881 gccatccagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca
     2941 agctggcgct atctgggcat cggggaggaa gacgcccgtg ccttttcccg cgaggttgaa
     3001 gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc
     3061 acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc
     3121 gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc
     3181 agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt
     3241 gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagtgagga cggctatcct
     3301 ggctggatgc cgcagaaatg gacatggata ccccgtgagt taccgggcgg tcgtgcttcg
     3361 ttcattcacg tctttgaacc cgtggaggac gggcagaccc gcggtgcaaa tgtgttttac
     3421 agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct gcagagcgcc
     3481 attgtgaagg cgatgtatgc cgccaccatc gagagtgagc tggatacgca gtcagcgatg
     3541 gattttattc ttggcgcgaa cagtaaggag cagcgggaca ggctgaccgg ctggattggt
     3601 gaaattgccg cgtattacgc cgcagcaccg gtccggctgg gaggcgcaaa agtgcctcac
     3661 ctgatgccgg gtgactcact gaacctgcag acggctcagg acacggataa cggctactcc
     3721 gtgtttgagc agtcactgtt gcggtatatc gctgccgggc tgggtgtctc gtatgagcag
     3781 ctttcccgga attacgccca gatgagctac tccacggcac gggccagtgc gaacgagtcg
     3841 tgggcgtact ttatggggcg gcgaaaattc gtcgcatccc gtcaggcgag ccagatgttt
     3901 ctgtgctggc tggaagaggc catcgctcgc cgcgtggtga cgttaccttc aaaagcgcgc
     3961 ttcagttttc aggaagcccg aagtgcctgg gggaactgcg actggatagg ctccggtcgt
     4021 atggccatcg atggtctgaa agaagtacag gaagcggtga tgctgataga agccggactg
     4081 agtacctacg agaaagagtg cgcaaaacgc ggtgacgact atcaggaaat ttttgcccag
     4141 caggtccgtg aaacgatgga gcgccgtgca gccggtctta aaccgcccgc ctgggcggct
     4201 gcggcatttg aatccgggct gcgacaatca acagaggagg agaagagtga cagcagagct
     4261 gcgtaatctc ccgcatattg ccagtatggc ctttaatgag ccgctgatgc ttgaacccgc
     4321 ctatgcgcgg gttttctttt gtgcgcttgc aggccagctt gggatcagcc gcctgacgga
     4381 tgcggtatcc ggcgacagcc tgactgccgg agaggcaccc gcggcgctgg cgttatccgt
     4441 tgatgatgac ggaccacgac aggcccgcag ttatcaggtc atgaacggca tcgccgtgct
     4501 gccggtgtcc ggcacgctgg tcagtcggac gcgagcgctg cagccgtatt cggggatgac
     4561 cggttacaac ggcattatcg cccgtctgca acaggctgcc agcgacccga tggtggacgg
     4621 cattctgctc gatatggata cgccaggcgg gatggtggca ggggcatttg actgcgctga
     4681 catcatcgcc cgtgtgcgtg acataaagcc ggtatgggcg ctggccaacg acatgaactg
     4741 cagtgcaggt cagttgcttg ccagcgccgc ctcccgacgt ctggtcacgc agaccgcccg
     4801 gacaggctcc atcggcgtca tgatggctca cagtaattac ggtgctgcgc tggagaaaca
     4861 gggcgtggaa atcacgctga tttacagcgg cagccataag gtggatggca acccctacag
     4921 ccatctaccg gatgatgtcc gggaaacact gcagtcccgg atggatgcaa cccgccggat
     4981 gtttgcacag aaggtgtcgg catataccgg cctgtccgtg caggctgtgc tggataccga
     5041 ggctgcagtg tacagcggtc aggaggccat tgatgccgga ctggctgatg aacttgttaa
     5101 cagtaccgat gcgatcaccg tcatgcgtga tgcactggat gcacgtaaat cccgtctctc
     5161 aggagggcga atgaccaaag agactcaatc aacaactgtt tcagccactg cttcgcaggc
     5221 tgacgttact ggcgtggtgc cagcgacgga gggcgagaac gccagcgctg cgcagccgga
     5281 cgtgaacgcg cagatcaccg cagcggttgc ggcagaaaac agtcgcatta tggggatcct
     5341 caactgtgag gaagctcacg gacgcgaaga acaggcacgc gtgctggccg aaacccctgg
     5401 tatgaccgtg gaaacggccc gccgcattct ggcagttgca ccacagagtg cacaggcgcg
     5461 cagtgacact gcgctggatc gtctgatgca gggggcaccg gcaccgctgg ctgcaggtaa
     5521 cctggcatct gatgccgtta acgatttgct gaacacacca gtgtaaggga tgtttatgac
     5581 gagcaaagaa acctttaccc attaccagcc gctgggcaac agtgacccgg ctcataccgc
     5641 aaccgcgccc ggcggattga gtgcgaaagc gcctgcaatg accccgctga tgctggacac
     5701 ctccagccgt aagctggttg cgtgggatgg caccaccgac ggtgctgccg ttggcattct
     5761 tgcggttgct gctgaccaga ccagcaccac gctgacgttc tacaagtccg gcacgttccg
     5821 ttatgaggat gtgctctggc cggaggctgc cagcgacgag acgaaaaaac ggaccgcgtt
     5881 tgccggaacg gcaatcagca tcgtttaact ttacccttca tcactaaagg ccgcctgtgc
     5941 ggcttttttt acgggatttt tttatgtcga tgtacacaac cgcccagctg ctggcggcaa
     6001 atgagcagaa atttaagttt gatccgctgt ttctgcgtct ctttttccgt gagagctatc
     6061 ccttcaccac ggagaaagtc tatctctcac aaattccggg actggtaaac atggcgctgt
     6121 acgtttcgcc gattgtttcc ggtgaggtta tccgttcccg tggcggctcc acctctgaat
     6181 ttacgccggg atatgtcaag ccgaagcatg aagtgaatcc gcagatgacc ctgcgtcgcc
     6241 tgccggatga agatccgcag aatctggtgg acccggctta ccgccgccgt cgcatcatca
     6301 tgcagaacat gcgtgacgaa gagctggcca ttgctcaggt cgaagagatg caggcagttt
     6361 ctgccgtgct taagggcaaa tacaccatga ccggtgaagc cttcgatccg gttgaggtgg
     6421 atatgggccg cagtgaggag aataacatca cgcagtccgg cggcacggag tggagcaagc
     6481 gtgacaagtc cacgtatgac ccgaccgacg atatcgaagc ctacgcgctg aacgccagcg
     6541 gtgtggtgaa tatcatcgtg ttcgatccga aaggctgggc gctgttccgt tccttcaaag
     6601 ccgtcaagga gaagctggat acccgtcgtg gttctaattc cgagctggag acagcggtga
     6661 aagacctggg taaagcggtg tcctataagg ggatgtatgg cgatgtggcc atcgtcgtgt
     6721 attccggaca gtacgtggaa aacggcgtca aaaagaactt cctgccggac aacacgatgg
     6781 tgctggggaa cactcaggca cgcggtctgc gcacctatgg ctgcattcag gatgcggacg
     6841 cacagctcga aggcattaac gcctctgccc gttacccgaa aaactgggtg accaccggcg
     6901 atccggcgcg tgagttcacc atgattcagt cagcaccgct gatgctgctg gctgaccctg
     6961 atgagttcgt gtccgtacaa ctggcgtaat catggccctt cggggccatt gtttctctgt
     7021 ggaggagtcc atgacgaaag atgaactgat tgcccgtctc cgctcgctgg gtgaacaact
     7081 gaaccgtgat gtcagcctga cggggacgaa agaagaactg gcgctccgtg tggcagagct
     7141 ggaagaggag cttgatgaca cggatgaaac tgccggtcag gacacccctc tcagccggga
     7201 aaatgtgctg accggacatg aaaatgaggt gggatcagcg cagccggata ccgtgattct
     7261 ggatacgtct gaactggtca cggtcgtggc actggtgacg ctgcatactg atgcacttca
     7321 cgccacgcgg gatgaacctg tggcatttgt gcttccggga acggcgttcc gtgtctctgc
     7381 cggtgtggca gccgaaatga cagaacgtgg cctggccaga atgcaataac gggaggcgct
     7441 gtggctgatt tcgataacct gttcgatgct gccattgtcc gcgccgatga aacgatacgc
     7501 gggtacatgg gaacgtcagc caccatgaca tccggtgagc agtccggcgc agtaatacgt
     7561 ggtgtttttg atgaccctga aaatatcagc tatgccggac agggcgtgcg cgttgaaggc
     7621 tccagcccgt ccctgtttgt ccggactgat gatgtgcggc agctgcggcg cggcgacacg
     7681 ctgaccatcg gtgaggaaaa cttctggata gaccggattt cgacggatga tggcggaagc
     7741 tgtcatctct ggcttgggcg tggcgtgccg cctgccgtta accgtcgtcg ctgaaggggg
     7801 gatgtatggc cataaaaggt cttgagcagg ccgttgaaaa cctcagccgt atcagcagaa
     7861 cggcggtgcc cggtgccgcc gcaatggcca ttaaccgcgt tgcttcatcc gcgatatcgc
     7921 agtcggcgtc acaggttgcc cgtgagacaa aggtacgccg gaaactggta aaggaaaggg
     7981 ccaggctgaa aagggccacg gtcaaaaatc cgcaggccag aatcaaggtt aaccgggggg
     8041 atttgcccgt aatcaggctg ggtaacgcgc gggttgtcct gtcccgacgc aggcgtcgta
     8101 aaaaggggca gcgttcatcc ctgaaaggtg gcgacagcgt gcttgtggtg ggaaaccgtc
     8161 gtattcccgg cgcgtttatt cagcaactga aaaatggccg atggcatgtc atgcagcgtg
     8221 tggccgggaa aaaccgttac cccattgatg tggtgaaaat cccgatggca gtgccgctga
     8281 ccacggcgtt taaacagaat attgagcgga tacggcgtga acgtcttccg aaagagctgg
     8341 gctatgcgct gcagcatcaa ctgaggatgg taataaagcg atgaaacata cagaactccg
     8401 tgcagccgta ctggatgcac tggaaaaaca tgacaccggg gcgacgcttt ttgatggtcg
     8461 ccccgctgtt tttgatgagg aggattttcc ggcagttgcc gtttatctca ccggcgctga
     8521 atacacgggc gaagagctgg acagcgatac ctggcaggcg gagctgcata ttgaagtttt
     8581 cctgcctgct caggtgccgg attcagagct ggattcgtgg atggagtccc ggatttatcc
     8641 ggtgatgagc gatgtcccgg cactgtcaga tttgatcacc agtatggtgg ccagtggcta
     8701 tgactaccgg cgcgacgatg atgcgggcct gtggagttca gccgatctga cttatgtcat
     8761 tacctatgaa atgtgaggac gatatgcctg taccaaatcc agtaatgccg gtgaaagggg
     8821 ccgggaccac cctgtgggtt tataagggga acggtgaccc ttatgcgaac ccgctttcag
     8881 acgttgactg gtcgcgtctg gctaaagtta aagacctgac gcccggcgaa ctgagcgctg
     8941 agtcctatga cgacagctat ctcgatgatg aagatgcgga ctggactgcg accgggcagg
     9001 ggcagaaatc tgccggagat accagcttca cgctggcgtg gatgcccgga gagcaggggc
     9061 agcaggcgct gctggcgtgg tttaatgaag gggatacccg tgcctataaa atccgcttcc
     9121 cgaacggcac ggtcgatgtg ttccgcggct gggtcagcag tatcggtaag gcggtgacgg
     9181 cgaaggaagt gatcacccgc acggtgaaag tcaccaacgt gggacgtccg tcgatggcag
     9241 aagatcgcag cacggtgacg gcgacaaccg gcatgacggt gacacccgcc agtgcttccg
     9301 tagtgaaagg gcagagcact acgctgaccg tggcattcca gccggagggc gcaaccgaca
     9361 agagcttccg tgcggtgtct gcggataaaa caaaagccac cgtgtcggtc agcggtatga
     9421 ccatcaccgt gaacggcgtt gctgcaggca aggtcaacat tccggttgta tccggtaatg
     9481 gtgagtttgc tgcggttgca gaaatcaccg tcaccgccag ttaatccgga gagtcagcga
     9541 tgttcctgaa aaccgaatca tttgaatata acggcgtgac cgtcacgctt tctgaactgt
     9601 cagccctgca gcgtattgag catcttgccc tgatgaaacg gcaggcagaa ccggcgggat
     9661 ccgacagcaa ccggcaggtt actgtggaag acgtcatcag aaccggcgcg tttctggtgg
     9721 cgatgtccct gtggcataac catccgaaga agacgcagat gccgtccatg aatgaagccg
     9781 ttaaacagat tgagcaggaa gtgcttacca cctggcccac agaggcaatt tctcatgctg
     9841 aaaacgtggt gtaccggctg tccggtatgt atgagtttgt ggtgaataat gcccccgaac
     9901 aggcagagga cgccgggcct gcagagcctg tttctgcggg aaagtgttcg acggtgagct
     9961 gagttttgcc ctgaaactgg cgcgtgagat ggggcgaccc gactggcgtg ccatgcttgc
    10021 cgggatgtca tccacggagt atgccgactg gcaccgcttt tacagtaccc attattttca
    10081 tgatgttctg ctggatatgc acttttccgg gctgacgtac accgtgctca gcctgttttt
    10141 cagcgatccg gatatgcatc cgctggattt cagtctgctg aaccggcgcg aggctgacga
    10201 agagcctgaa gatgatgtgc tgatgcagaa agcggcaggg cttgccggag gtgtccgctt
    10261 tggcccggac gggaatgaag ttatccccgc ttccccggat gtggcggaca tgacggagga
    10321 tgacgtaatg ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac
    10381 cggtaggcga tctggtcgtt gatttgagtc tggatgcggc cagatttgac gagcagatgg
    10441 ccagagtcag gcgtcatttt tccggtacgg aaagtgatgc gaaaaaaaca gcggcagtcg
    10501 ttgaacagtc gctgagccga caggcgctgg ctgcacagaa agcggggatt tccgtcgggc
    10561 agtataaagc cgccatgcgt atgctgcctg cacagttcac cgacgtggcc acgcagcttg
    10621 caggcgggca aagtccgtgg ctgatcctgc tgcaacaggg ggggcaggtg aaggactcct
    10681 tcggcgggat gatccccatg ttcagggggc ttgccggtgc gatcaccctg ccgatggtgg
    10741 gggccacctc gctggcggtg gcgaccggtg cgctggcgta tgcctggtat cagggcaact
    10801 caaccctgtc cgatttcaac aaaacgctgg tcctttccgg caatcaggcg ggtctgacgg
    10861 cagatcgtat gctggtcctg tccagagccg ggcaggcggc agggctgacg tttaaccaga
    10921 ccagcgagtc actcagcgca ctggttaagg cgggggtaag cggtgaggct cagattgcgt
    10981 ccatcagcca gagtgtggcg cgtttctcct ctgcatccgg cgtggaggtg gacaaggtcg
    11041 ctgaagcctt cgggaagctg accacagacc cgacgtcggg gctgacggcg atggcacgcc
    11101 agttccataa cgtgacggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg
    11161 atgaagccgg ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga
    11221 cccgccgcct gaaagagaac atgggcacgc tggagacctg ggcagacagg actgcgcggg
    11281 cattcaaatc catgtgggat gcggtgctgg atattggtcg tcctgatacc gcgcaggaga
    11341 tgctgattaa ggcagaggct gcgtttaaga aagcagacga catctggaat ctgcgcaagg
    11401 atgattattt tgttaacgat gaagcgcggg cgcgttactg ggatgatcgt gaaaaggccc
    11461 gtcttgcgct tgaagccgcc cgaaagaagg ctgaacagca gagtcaacag gacaaaaatg
    11521 cgcagcagca gagcgatacc gaagcgtcac ggctgaaata taccgaagag gcgcagaagg
    11581 cttacgaacg gctgcagacg ccgctggaga aatatgccgc ccgtcaggaa gaactgaata
    11641 aggccctgaa agacggaaaa atcctgcagg cagattacaa cacgctgatg gcggcagcga
    11701 aaaaggacta tgaaacgaca ctgaaaaagc cgaagcagtc cggcgtgaag gtgtctgcgg
    11761 gcgatcgtca ggaagacagt gctcatgctg ccctgctgac gcttcaggct gaactccgga
    11821 cgctggagaa gcatgccgga gcaaatgaga aaatcagcca gcagcgccgg gatttgtgga
    11881 aggcggagag tcagttcgcg gtactggagg aggcggcgca acgtcgccag ctgtctgtac
    11941 aggagaaatc cctgctggcg cataaagatg agacgctgga gtacaaacgc cagctggctg
    12001 cacttggcga caaggttacg tatcaggagc gcctgaacgc gctggcgcag caggcggata
    12061 aattcgcaca gcagcaacgg gcaaaacggg ccgccattga tgcgaaaagc cgggggctga
    12121 ctgaccggca ggcagaacgg gaagccacgg aacagcgcct gaaggaacag tatggcgata
    12181 atccgctggc gctgaataac gtcatgtcag agcagaaaaa gacctgggcg gctgaagacc
    12241 agcttcgcgg gaactggatg gcaggcctga agtccggctg gagtgagtgg gaagagagcg
    12301 ccacggacag tatgtcgcag gtaaaaagtg cagccacgca gacctttgat ggtattgcac
    12361 agaatatggc ggcgatgctg accggcagtg agcagaactg gcgcagcttc acccgttccg
    12421 tgctgtccat gatgacagaa attctgctta agcaggcaat ggtggggatt gtcgggagta
    12481 tcggcagcgc cattggcggg gctgttggtg gcggcgcatc cgcgtcaggc ggtacagcca
    12541 ttcaggccgc tgcggcgaaa ttccattttg caaccggagg atttacggga accggcggca
    12601 aatatgagcc agcggggatt gttcaccgtg gtgagtttgt cttcacgaag gaggcaacca
    12661 gccggattgg cgtggggaat ctttaccggc tgatgcgcgg ctatgccacc ggcggttatg
    12721 tcggtacacc gggcagcatg gcagacagcc ggtcgcaggc gtccgggacg tttgagcaga
    12781 ataaccatgt ggtgattaac aacgacggca cgaacgggca gataggtccg gctgctctga
    12841 aggcggtgta tgacatggcc cgcaagggtg cccgtgatga aattcagaca cagatgcgtg
    12901 atggtggcct gttctccgga ggtggacgat gaagaccttc cgctggaaag tgaaacccgg
    12961 tatggatgtg gcttcggtcc cttctgtaag aaaggtgcgc tttggtgatg gctattctca
    13021 gcgagcgcct gccgggctga atgccaacct gaaaacgtac agcgtgacgc tttctgtccc
    13081 ccgtgaggag gccacggtac tggagtcgtt tctggaagag cacgggggct ggaaatcctt
    13141 tctgtggacg ccgccttatg agtggcagca gataaaggtg acctgcgcaa aatggtcgtc
    13201 gcgggtcagt atgctgcgtg ttgagttcag cgcagagttt gaacaggtgg tgaactgatg
    13261 caggatatcc ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc ggccagcgtg
    13321 gtgctctggg aaatcgacct gacagaggtc ggtggagaac gttatttttt ctgtaatgag
    13381 cagaacgaaa aaggtgagcc ggtcacctgg caggggcgac agtatcagcc gtatcccatt
    13441 caggggagcg gttttgaact gaatggcaaa ggcaccagta cgcgccccac gctgacggtt
    13501 tctaacctgt acggtatggt caccgggatg gcggaagatc tgcagagtct ggtcggcgga
    13561 acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga
    13621 aacagttatg ccgatccgga gcaggaggtg atcagccgct ggcgcattga gcagtgcagc
    13681 gaactgagcg cggtgagtgc ctcctttgta ctgtccacgc cgacggaaac ggatggcgct
    13741 gtttttccgg gacgtatcat gctggccaac acctgcacct ggacctatcg cggtgacgag
    13801 tgcggttata gcggtccggc tgtcgcggat gaatatgacc agccaacgtc cgatatcacg
    13861 aaggataaat gcagcaaatg cctgagcggt tgtaagttcc gcaataacgt cggcaacttt
    13921 ggcggcttcc tttccattaa caaactttcg cagtaatccc atgacacaga cagaatcagc
    13981 gattctggcg cacgcccggc gatgtgcgcc agcggagtcg tgtggcttcg tggtgagaac
    14041 gccg
//
LOCUS       DAOFSV010000120        13977 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.120, whole genome shotgun sequence.
ACCESSION   DAOFSV010000120 DAOFSV010000000
VERSION     DAOFSV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.120"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            182..256
                     /gene="tnaC"
                     /locus_tag="QBH27_003599"
     CDS             182..256
                     /gene="tnaC"
                     /locus_tag="QBH27_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDQ7015867.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            477..1892
                     /gene="tnaA"
                     /locus_tag="QBH27_003600"
     CDS             477..1892
                     /gene="tnaA"
                     /locus_tag="QBH27_003600"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDQ7015868.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDNSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1984..3231
                     /gene="tnaB"
                     /locus_tag="QBH27_003601"
     CDS             1984..3231
                     /gene="tnaB"
                     /locus_tag="QBH27_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDQ7015869.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3363..4538
                     /gene="mdtL"
                     /locus_tag="QBH27_003602"
     CDS             3363..4538
                     /gene="mdtL"
                     /locus_tag="QBH27_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDQ7015870.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHNA"
     gene            4513..5472
                     /gene="yidZ"
                     /locus_tag="QBH27_003603"
     CDS             4513..5472
                     /gene="yidZ"
                     /locus_tag="QBH27_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDQ7015871.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSSVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLSIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5629..6378
                     /locus_tag="QBH27_003604"
     CDS             5629..6378
                     /locus_tag="QBH27_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDQ7015872.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6400..6966
                     /gene="yieF"
                     /locus_tag="QBH27_003605"
                     /gene_synonym="chrR"
     CDS             6400..6966
                     /gene="yieF"
                     /locus_tag="QBH27_003605"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDQ7015873.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(7019..8356)
                     /gene="adeP"
                     /locus_tag="QBH27_003606"
     CDS             complement(7019..8356)
                     /gene="adeP"
                     /locus_tag="QBH27_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDQ7015874.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8523..9188
                     /gene="yieH"
                     /locus_tag="QBH27_003607"
     CDS             8523..9188
                     /gene="yieH"
                     /locus_tag="QBH27_003607"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDQ7015875.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIINIISKEQGVTMIQADAEHVYRAEVARLFDSELEVIAGANELLASVKV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9255..9722
                     /locus_tag="QBH27_003608"
     CDS             9255..9722
                     /locus_tag="QBH27_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015876.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLWVIIH
                     PIFLLLSITTGAIPALLTGVMVACLPEKIGSQKRYRCLAGGIGGVVITEIYCAVIIHI
                     KVMASSVLFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9771..10358
                     /locus_tag="QBH27_003609"
     CDS             9771..10358
                     /locus_tag="QBH27_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDQ7015877.1"
                     /translation="MTQNIRPLPLFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIANGSAAEKFAGSFQDDASIEGVEFEYDEEDDFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10419..11141)
                     /locus_tag="QBH27_003610"
     CDS             complement(10419..11141)
                     /locus_tag="QBH27_003610"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDQ7015878.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11156..12325)
                     /locus_tag="QBH27_003611"
     CDS             complement(11156..12325)
                     /locus_tag="QBH27_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDQ7015879.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGINHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12352..13968)
                     /gene="bglH"
                     /locus_tag="QBH27_003612"
     CDS             complement(12352..13968)
                     /gene="bglH"
                     /locus_tag="QBH27_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDQ7015880.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSANDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
BASE COUNT         3493 a         3412 c         3352 g         3720 t
ORIGIN      
        1 ctgttattcc tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa
       61 atagatattt aatattgcgt cccgaacgat tgtgattcga ttcacattta aacaatttca
      121 gaatagacaa atttcttgag tattatactg tagcctcgtg tctttcgagg ataagtgcat
      181 tatgaatatc ttacatatat gtgtgacctc aaaatggttt aatattgaca acaagattgt
      241 cgatcaccgc ccttgatttg cccttctgta gccatcacca gagccaaacc gattagattc
      301 aatgtgatcg attagtttgc tatatcttaa ttttgccttt tacaaaggcc atctctcgtt
      361 tatttacttg ttttagtaaa tgatggtgct tgcatatata tctggcgaat taatggatat
      421 tgcaaatgta atattcacag ggatcactgt aattaaaata aatgaaggat tatgtaatgg
      481 aaaactttaa acatctccct gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta
      541 ccactcgcgc ttatcgtgaa gaggcaatta ttaaatccgg tatgaacccg ttcctgctgg
      601 atagcgaaga tgtgtttatc gatttactga ccgacagcgg caccggggcg gtgacgcaga
      661 gtatgcaggc cgcgatgatg cgtggcgacg aagcctacag cggcagccgc agctactacg
      721 cgttagccga gtcagtgaaa aatatctttg gttatcaata cactattccg actcaccagg
      781 gccgtggtgc agagcaaatc tatattccgg tactgattaa aaaacgcgag caggaaaaag
      841 gcctggatcg cagcaaaatg gtggcgttct ctaactattt ctttgatacc acgcagggcc
      901 atagccagat taacggctgt accgtgcgta acgtctatat caaagaagcc ttcgatactg
      961 gcgtgcgtta cgactttaaa ggcaactttg accttgaggg attagaacgc ggtattgaag
     1021 aagttggccc gaataacgtg ccgtatattg ttgcaaccat caccagtaac tccgcaggtg
     1081 gtcagccggt ttcactggca aacttaaaag cgatgtacag catcgcgaag aaatacgata
     1141 ttccggtggt catggactcc gcacgctttg ccgaaaacgc ctatttcatc aagcagcgtg
     1201 aagcagaata caaagactgg accatcgagc agatcacccg cgaaacctac aaatatgccg
     1261 atatgctggc gatgtccgcc aagaaagatg cgatggtgcc gatgggcggc ctgctgtgca
     1321 tgaaagacaa cagcttcttt gatgtgtata ccgaatgcag aaccctttgc gtggtgcagg
     1381 aaggcttccc gacatatggc ggcctggaag gcggcgctat ggagcgtctg gcggtaggtc
     1441 tgtatgacgg catgaatctg gactggctgg cttatcgtat cgcgcaggtg cagtatctgg
     1501 tcgatggtct ggaagagatt ggcgttgtct gtcagcaggc gggcggtcac gcggcattcg
     1561 ttgatgcagg taaactgttg ccgcatatcc cggcagatca gttcccggca caggcgctgg
     1621 cctgcgagct gtataaagtc gccggtatcc gtgcggtaga aattggctct ttcctgttag
     1681 gccgcgatcc gaaaaccggt aaacaactgc catgcccggc tgaactgctg cgtttaacca
     1741 ttccgcgcgc aacatatact caaacacata tggacttcat tattgaagcc tttaaacatg
     1801 tgaaagagaa cgcggcgaat attaaagggt taacctttac ctacgaaccg aaagtattgc
     1861 gtcacttcac cgcaaaactg aaagaagttt aattaaaaca ctcagagtgg ctataaggat
     1921 gttagccact ctattaccct acatcctcaa taacaaaaat agccttcctc taaaggtggc
     1981 atcatgactg atcaatcaga aaaaaaacac tctgcatttt ggggtgttat ggttatagcc
     2041 ggtacagtaa ttggcggtgg gatgtttgct ttacctgttg accttgccgg agcctggttt
     2101 ttttggggcg cttttattct tattatcgcc tggttttcaa tgttacattc agggctatta
     2161 ttattagaag caaacttaaa ttatcccgtc ggttccagtt ttaacaccat caccaaagat
     2221 ttaatcggta acacctggaa cataatcagc ggtattaccg ttgccttcgt tctctatatc
     2281 ctcacttatg cctatatctc tgctaatggt gcgatcatta gtgaaacgat atcaatgaat
     2341 ttgggctatc acgctaatcc acgtattgtc gggatctgca ctgccatttt cgttgccagc
     2401 gtattgtgga taagttcatt agccgccagt cgtattacct cattgttcct tgggctgaag
     2461 attatctcct ttgtaattgt ttttggttct ttcttcttcc aggtcgatta ctccattctg
     2521 cgcgattcca ccagcaccac tgcggggacg tcttacttcc cgtatatctt tatggctttg
     2581 ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc ccagcctgat tatttgctat
     2641 ggcaaacgca aagataagct catcaaaagc gtggtcttcg gttcgctgct ggcgctggtg
     2701 atttatctct tctggctcta ttgcacgatg gggaatatcc cgcgagaaag ctttaaggcg
     2761 ataatctcct caggcggcaa cgttgattcg ctggtgaaat cgttcctcgg caccaaacag
     2821 cacggcatta tcgaattttg cctgctggtg ttctctaact tagctgttgc cagctcgttt
     2881 tttggtgtca cgctgggatt gttcgattat ctggcggatc tgtttaagat tgataactcc
     2941 cacgccgggc gtttcaaaac cgtgctgtta accttcctgc cacccgcgtt gttgtatctg
     3001 atcttcccga acggctttat ttacgggatc ggcggtgccg gactgtgcgc caccatctgg
     3061 gcggtaataa ttcctgctgt actggcaatc aaagcccgca agaagtttcc caatcagatg
     3121 ttcacggtct ggggcggcaa tcttattccg gcgattgtca ttctctttgg cataaccgta
     3181 attttatgct ggttcggtaa cgtctttaac gtgttaccta aatttggcta aatctttcag
     3241 gaagccagcc attcgctggc ttcttgcctg tcaggaaatc tcttttgtcc aaatggcaac
     3301 tcgcctgatt atccttcacc acgtatgctt tgcgtcacct tactatcagg acgctttagc
     3361 ccatgtcccg ctttttgatt tgtagttttg ccctggtttt actttatccc gcagggattg
     3421 atatgtacct cgttggttta ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt
     3481 tgcatattgc gttctccgtt tatctggcgg ggatggcggc tgcgatgtta tttgccggta
     3541 aagtggccga tcgttcaggg agaaagcctg tcgccatacc cggcgcggcg ctatttatta
     3601 tcgcctcggt gttctgttca ctggctgaaa ccagcgcgtt atttcttgca gggcgatttc
     3661 tacagggact gggcgcgggc tgttgttacg tagtggcgtt cgccattttg cgcgacacgc
     3721 tggatgatcg acgtcgggct aaagtgctgt cgttactcaa cggcattacc tgcatcattc
     3781 cggtgttagc gccggtgctc ggacacctga ttatgcttaa attcccttgg cagagtctgt
     3841 tctggacgat ggcaacgatg ggcatcgcgg tactgatgtt gtctttgttt attttaaaag
     3901 aaacgcgccc tgcggccccc gcagcttcgg acaaaccacg agaaaatagc gagtcactgc
     3961 ttaaccggtt tttcctcagc cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca
     4021 ccttcgtcaa cacgtcgcca gtattgctga tggaaatcat gggttttgag cgcggagaat
     4081 acgccaccat tatggcgttg accgctggcg tcagcatgac cgtttcattc tccacgccgt
     4141 ttgcgctggg aatttttaag ccacgtacgt tgatgatcac ctcacaggtg ttattcctgg
     4201 cggcggggat cacccttgcc gtttcacctt cccatgcggt ttcgctgttt ggtatcacgc
     4261 tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat gagtcaggcg ttggggccgt
     4321 tttcattacg cgcgggcgta gccagctcga ccttaggtat tgcgcaagtt tgcggttcgt
     4381 cactgtggat ttggctggcg gcggtggttg gtatcggtgc atggaatatg ctgatcggga
     4441 ttctgattgc ctgtagcata gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc
     4501 cggttgccgc tcatgaagaa atccatcaca acgcttgatc tcaatctgct gctctgtctg
     4561 caactgctga tgcaggagcg cagcgtgacc aaagcggcga agcggatgaa cgtgacccct
     4621 tcgtcggtga gtaagtcgct ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg
     4681 aactcaccgc tggggctgtc gcccacaccg ctaatggtca gcatggagca aaatctggcg
     4741 gagtggatgc aaatgagcaa tcagttgctg gacaaaccac tccacgaaac gccgcgcggt
     4801 cttaagttcg aactggcggc ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg
     4861 aaacggatct accaacgata cccgcaggcg accatcaaat tacgtaactg ggattacgat
     4921 tccttagatg ccattactcg tggtgaagtg gatattggtt tttccggtcg cgaaagtcat
     4981 ccccgttcgc gggagctgtt aagctcgcta ccgttatcca ttgattatga agtgctgttt
     5041 agtgatgtgc cctgcgtctg gttacgcaaa gatcatccgg cactgcatga agcatggaat
     5101 ctggacacct ttttacgtta tccgcatatc agcatttgct gggaacagag cgatacctgg
     5161 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
     5221 gaattcgagc agtcactgtt tatggcagca cagcccgaca atctgctact ggcgaccgcg
     5281 ccgcgctact gccagtacta caatcaactc caccaactgc cgttggttgc tcttcctctc
     5341 ccgtttgatg aaagccagca aaaaaagctg gaagtccctt ttacccttct gtggcataaa
     5401 cggaacagcc gtaatccgaa gatcgtctgg ttacgggaaa ccattaaaaa tctttacgcg
     5461 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaatgaaa tgtaaaaaag
     5521 ttgtaataaa tttatctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
     5581 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
     5641 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
     5701 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
     5761 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
     5821 ctgccgaaag gaaaaccggt tttcagcgat aaaaatttgc cttcgttttc tatttcctat
     5881 gccgggaata tggtcggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
     5941 ctacagcgtg cgacacgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
     6001 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
     6061 atcacgctgc gccggagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
     6121 gatctgcaac tactgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     6181 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa
     6241 aagctcagtg tctgggagtt agatggcaaa cacggctgga aaagcctgcc cgatattcac
     6301 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     6361 gctttttcgc caaattgata gacaaccaca ggagtcatca tgtctgaaaa attgcaggtg
     6421 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     6481 ccgaaaattg ctccggcgag tatggaagtc aatgcgttgc cttccattgc cgacattccc
     6541 ctgtatgacg ctgacgtaca gcaggaagac ggttttccag caacggttga agctctggcg
     6601 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     6661 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggct
     6721 ggtaaaccgg tattgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     6781 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccagaattt
     6841 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gggaagtgat tgatcagggt
     6901 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     6961 atctaaataa aaacccgcca gcaatcatgc atggcgggtt tttaacgcgc tatcgatttt
     7021 agtgagcgtc gataaagaca atcttcagga taaacagcag cgcaacgatg attacgcacg
     7081 ggctaaggtc acgcagacgt ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     7141 gcgcgatacc ttcagtaatc gagaagctga acggcatcat cacagcggta ataaacgccg
     7201 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     7261 caacgtaaat cagcgcacca gctgcagcgt agcctggcac catccccgcc agcggcgaca
     7321 ggaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     7381 caacggaaac gccggaagaa gattcaatat acgccgtaac agaagaagta ccgataaacg
     7441 aaccggtcac cgaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttac
     7501 ctttctcatc agccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     7561 acaagttgac cagcatgaaa gagaaaatca cccctgccag cccaaggtta aacgacccgg
     7621 ctaaatctac atgacccacg acactcatta cgctcggcgg cgcagaaacg atgccattat
     7681 agtggacatc acccagcatc cagcccagca gcgttgtcac cacgatggaa accagcaccg
     7741 ccgcgtgaat attgcgcgag gccagaatgg cgatgatgaa gaagccgagg atccccagca
     7801 gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgggtta gcgacaatca
     7861 cacctgcgtt tttcagcccc atcatgccaa taaacagacc gataccactg gtgatgccca
     7921 cacgtagact taccgggatg ttggctatca tccagtagcg aacgcggaaa atcgtcagta
     7981 acagcagacc aatcgcgccc cagaagattg cgcccatccc gacttgccac ggcaagccca
     8041 tcgcctgtac aacgacaaaa gcgaagaacg cattcaggcc cattgccggt gccagtgcaa
     8101 ctggcaggtt agcaaacagc cccatcataa tactgccgaa tgcagcaatc agacaggtcg
     8161 tgacgaagac ggcgctggta tccatgccag caacgccaag aatttgcggg ttaacaaaaa
     8221 cgatgtaaac catcgtcagg aaggtggtaa aaccggcgat cacttcggtc cgtgccgtcg
     8281 tgccatgttc gcgcagttta aacacgcgtt ccagcatccc ctggccagaa gcctgggttg
     8341 tgtgttgttg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     8401 ccaaaatgag acaatatgga cggctgaaga taactttttt tcattgtatt tgcttatacg
     8461 gcaacgattg cgtcatgcga attggcatga cgaaaacgtt aaactaacga taaaggaaag
     8521 acatgtccca gatagaagcg gtatttttcg actgcgacgg tacgctggtc gatagcgagg
     8581 tcatttgctc tcgcgcctat gtcacgatgt tcagggaatt tggcattcac gttgacctgg
     8641 aagagatttt tacccgtttt aaaggcgtaa aactctacga aatcatcaac atcatcagta
     8701 aagaacaggg cgtgacgatg atccaggccg atgccgagca cgtttaccgt gcggaagtgg
     8761 cgcgcctgtt tgatagcgag cttgaggtga ttgcaggagc gaacgaattg ctggctagtg
     8821 tgaaagtgcc gatgtgcgtc gtctctaacg gcccggtcag caagatgcag cattcgcttg
     8881 gtaagctcaa catgctgcac tacttcccgg aaaaactgtt cagcggctat gatatccagc
     8941 gttggaagcc cgatccggca ttaatgttcc atgcggcaaa agcgatgaat gtgaacgttg
     9001 aaaactgcat tctggtcgat gactccagtg ccggtgcgca atcagggata gatgccggaa
     9061 tggaagtgtt ttacttctgc gctgacccgc acaataaacc gatcgttcac ccgaaagtca
     9121 ccacctttac ccatctttcg caattaccgg aactgtggaa ggcgcgtggt tgggatatta
     9181 cggcatagtt attcacactc ccttcactta ctccacttaa attggcacgc aaaggtaagt
     9241 aaagggagtt tgatatgtct gtttcacgtc gggtaattca tcacggactg tattttgcgg
     9301 ttttagggcc gttaattggt gttctgtttc ttgtcctcta catattcttc gcaaaagaac
     9361 cgctggttct ttgggtgata atacatccaa tttttctctt attgtcgata actacgggag
     9421 ctattcctgc gttgttaacc ggtgtaatgg ttgcctgtct gccggaaaag atcgggtcac
     9481 agaaacgtta tcgttgtctg gctggtggca taggtggagt tgttatcacc gagatctatt
     9541 gcgctgttat catacatatt aaggtcatgg cttcctcagt gttgtttgaa aacattcttt
     9601 ctggtgacag tctcgttgtc cgcatcattc ctgcattgct ggcaggtgtg gtgatgagca
     9661 gaatcattac acgtctaccc ggactggata tttcatgtcc tgaaacagac tctttaagtt
     9721 aagcgggatg ttttatcttt tggctactca aaagcagaca ggatgtttct atgactcaaa
     9781 atatcaggcc gttaccccta ttcaaatatc atcccaagcc actggaaaca ggcgcatttg
     9841 aacaggataa aaccgtagag tgcgattgct gtgaacaaca gacgtcagtt tattactcgg
     9901 gtccctttta ttgcgttgat gaagttgaac atctctgtcc gtggtgtatt gcgaacggtt
     9961 ctgctgctga aaagtttgca ggtagttttc aggatgatgc cagcatcgaa ggtgttgaat
    10021 ttgagtatga tgaagaggac gattttgccg gtattaagaa cacatatcct gatgaaatgc
    10081 tgaaagagtt ggttgaacgc acgccaggtt atcatggatg gcaacaggaa ttctggctcg
    10141 cgcattgtgg cgatttctgt gcttttatcg gctatgtggg ctggaatgat ataaaagatc
    10201 gcctcgatga atttgccaac cttgaagaag attgtgagaa tttcggtatt agaaattctg
    10261 atctagctaa atgcctgcaa aagggtggtg attgtcaggg ttatctcttc cgctgtctcc
    10321 actgcggcaa gctgagactg tggggcgatt tttcatagtt atttatatga tgagaacagg
    10381 ccggcgcgta attcacacat ccggccttat ttttaagctt aaccgagcgc taattctgcg
    10441 gcagcggctt tatcagcgat taccatcagc gaaggatgca gctgcaacac ggaagcagga
    10501 acatcttcgg taaccggacc ttgcagaaca tttttcagtg cctgcgcttt acccgcgcca
    10561 ctgacaatta tcagcaagtt tttcgccgcc atgatgcttt tcggccccat cgtcacgtag
    10621 ctgtcaggca ctaatgaaaa gtcgccaccc aactcgccat gcgccacaat atcaaccatc
    10681 tctccctgga tcgggaactc cacggtttgc tcatggaaat gggtcgtatt tggcaaattg
    10741 ccacaaaaat gaccatctac acctaatccc aacaccacca aatccaggcc accttcacgc
    10801 gccagtttct gatcatgctc gcggtagtta tcaatagtga gcttctggat attctcttct
    10861 ttgatccctg caggggtgaa aaacagatta cgcagattgg taatcgttac gccttcccct
    10921 tctttgccac gaaatggaat ttcatcgaaa ttgtagaaat agcagttatc gtaccagggc
    10981 ttacctttaa ccagagtgat gagatattcg tacatgcctt tgggcgtgct accggcggta
    11041 attgccaggt taacgcgacg catctttgac atataaccaa gcagatgatg tgcggcgaca
    11101 cggctcattt cctggtaatc ttcggtaatg atcaatttca tcttaatgcc ttattttatt
    11161 taaataattt ctgtacaaag gctgcatagg ctggtcgcca gacatccatt tcatggttca
    11221 gaccgggata ttcctggtaa tcaaacttta tttttttctg ctcaagctca gttttcagcc
    11281 cggcgatatc cttgccggtt acgacatctt tatcgcccac gaccacagta aaattacgta
    11341 gttgctggtt aatagctgcc ggatcgttca gccgggcagc gaccccttca tctggtacgg
    11401 tcgtcgtggt aacaccactg aatgtggcca gccagccaaa gctttccaga tgattaattc
    11461 ccgataccag tgcctgatac ccaccttgtg aaagccctgc cagtgcgcga ccatcggcat
    11521 ctttacggac attaaaacgc ttgctaatca gcgggataat atcgttcatc agttcgcgat
    11581 ctgccgcttt agcatttaac ggataaaaga ctttacgtct ctcctgagga acgaaatctt
    11641 cgggaataat gcccttcgca tcggtttcgg tatccgggat caccaccagc atcggtttaa
    11701 ttttcccttc agccagcagg ttatccatga tttgcgggat acgcccctga tcaatagcgg
    11761 aacgtccggt atcaccaaag ccgtgataga aatagagcac tggcaaaggc tcgcctatgc
    11821 cggtgtatcc tggcggggtc cagacataca tctgacgttc ggattgcaat gcgttggagt
    11881 ggtaagttat ggcgatcaaa tcaccatgca caacagaacg tgtatccaga taactgcccg
    11941 gtaccagaat catactggag ttaacctgac gctgaggctt agtcattgcc gtgcctgtat
    12001 caatgctgcg tacaccatca acattgaaaa aatactcgta cagattgcct ttcaggacag
    12061 gcgtgcgcca cgaccagact cccgcttcat ctttggtcat cgggtgaata ttgtccggaa
    12121 ccggaacacc caccactacg gagacatttt ttgccccagg ggcaaagtag cgaaaggtca
    12181 cgctgttatc ggtattgacc tgagtcacat actgtttcac cggaatagtg ggtgccgggc
    12241 tggctggcat atccgctgct atggcccatt gagcagaaat accgctggca atagccagcg
    12301 ttaaagcagc aatttttata ttcatcgtaa tattcctttt attaagttcc attaccacca
    12361 gatttcagcc tgggcaccga cagcaaactg gtcgtcttta ttgtcttcaa aggtgaagcc
    12421 atccagttcg ttttccagag ctttgatata tgtagcgtag aaacgaattt ccgggcgcga
    12481 ggtcaacata ctggtattga ctttaaaggt atggaagagc gtggttttat aaccagactc
    12541 attatattta ttgctattcg catctttgtt ttgctgggtg aaatagccca gttcaacacc
    12601 tgtctggtta tattggtccc aaatataggc cgggcgaaca accgcacgaa tagattcgaa
    12661 atcagaatgt gcgcctgttt cgtagctata aatatcgtta ccgaaggagt aaacaatcgc
    12721 gttagccaca atgaaatgat cgccgatata agcttcgccc tgcgaagtca gacgaactgc
    12781 agttccgcct gtgtgatcac cataataacg accattaaag gtagtaaatg gacttgcacc
    12841 agcataacga ccaaagttac tggcgataga gttattcgca accaggaagg agaattcgtt
    12901 gaagccaccc ttatcaaatt tctgcgttaa agatgtacca aacatccagg tatctttcca
    12961 gtcataatac ccgttattat cctgattatc tttttccgat gcgctttcat ttgctgagac
    13021 ataacgaccg ctcaccatta aggtggcttt atcccataac gggatatctt tatagcgtaa
    13081 atcaatggta ttggtattaa tctgctgttt gttttgcagg ctgcgatcgt aatcatcgac
    13141 atcttcacga accagcgcga tatcaatttt acccggaccg actttccagt tttccagacc
    13201 tacacctgcg gcagcatcgg tacgctgcgt tttccagtca agcatctgga tttcaatttt
    13261 cggcgcaccg tgtttaccca cccagaaatc agcctctggc gcaaagggca ggaaaccttt
    13321 ggtggtaacg tacatatcag agaactgcat aaagttctcg ccaccggcgt tatcgccaaa
    13381 ccagccagta gagtactgct gacccacgtt accatccatc atcacgatgg catcaacccg
    13441 tttgccgttt tcgttgtaga cacgttgttt taactgcaaa tcaaaccagc cggagtattc
    13501 gttaccaaag cggcccagag aaccaattgc ccatgattta ggtgaaccat gggaggctgt
    13561 gccccaacca gaacggtagt agccgttgta actaaagccg atttcatctt tcacaaattt
    13621 actgaaatct ttcagagtca tggaagaata aatcgagcct gtctcactgg catttttctc
    13681 ttcattttta accagtacag catcaggctt tgcggcacga ctggtcggga atggattggc
    13741 ggcataccct tgatcattcg cacttacgct acgattcacc gtcgctggcg tatacttttt
    13801 cttctcttca tctttatatt ttttcagttc gctttgcgtt tctcttaacg ccttttctaa
    13861 tagctcaagg cgttgttcca cggttaatga ttcagccaat gattgtgcgg aaaaggctaa
    13921 cggtgccatt aataaaatgg cagaggtaat aatatttcgt ctaaacataa tatccct
//
LOCUS       DAOFSV010000121        13735 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.121, whole genome shotgun sequence.
ACCESSION   DAOFSV010000121 DAOFSV010000000
VERSION     DAOFSV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.121"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(36..1544)
                     /gene="putP"
                     /locus_tag="QBH27_003613"
     CDS             complement(36..1544)
                     /gene="putP"
                     /locus_tag="QBH27_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDQ7015881.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNGHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(1703..1912)
                     /locus_tag="QBH27_003614"
     CDS             complement(1703..1912)
                     /locus_tag="QBH27_003614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015882.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            1967..5929
                     /gene="putA"
                     /locus_tag="QBH27_003615"
     CDS             1967..5929
                     /gene="putA"
                     /locus_tag="QBH27_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDQ7015883.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDNALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(5969..6607)
                     /gene="rutR"
                     /locus_tag="QBH27_003616"
     CDS             complement(5969..6607)
                     /gene="rutR"
                     /locus_tag="QBH27_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDQ7015884.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            6895..7986
                     /gene="rutA"
                     /locus_tag="QBH27_003617"
     CDS             6895..7986
                     /gene="rutA"
                     /locus_tag="QBH27_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDQ7015885.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            7986..8678
                     /gene="rutB"
                     /locus_tag="QBH27_003618"
     CDS             7986..8678
                     /gene="rutB"
                     /locus_tag="QBH27_003618"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDQ7015886.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            8690..9076
                     /gene="rutC"
                     /locus_tag="QBH27_003619"
     CDS             8690..9076
                     /gene="rutC"
                     /locus_tag="QBH27_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDQ7015887.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            9084..9884
                     /gene="rutD"
                     /locus_tag="QBH27_003620"
     CDS             9084..9884
                     /gene="rutD"
                     /locus_tag="QBH27_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDQ7015888.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINTHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            9894..10484
                     /locus_tag="QBH27_003621"
     CDS             9894..10484
                     /locus_tag="QBH27_003621"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDQ7015889.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSDLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            10495..10989
                     /gene="rutF"
                     /locus_tag="QBH27_003622"
     CDS             10495..10989
                     /gene="rutF"
                     /locus_tag="QBH27_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDQ7015890.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEKALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            11010..12338
                     /gene="rutG"
                     /locus_tag="QBH27_003623"
     CDS             11010..12338
                     /gene="rutG"
                     /locus_tag="QBH27_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDQ7015891.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGITTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(12421..12594)
                     /locus_tag="QBH27_003624"
     CDS             complement(12421..12594)
                     /locus_tag="QBH27_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDQ7015892.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            12967..13563
                     /gene="wrbA"
                     /locus_tag="QBH27_003625"
     CDS             12967..13563
                     /gene="wrbA"
                     /locus_tag="QBH27_003625"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDQ7015893.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            13584..>13735
                     /locus_tag="QBH27_003626"
     CDS             13584..>13735
                     /locus_tag="QBH27_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015894.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEV"
BASE COUNT         3141 a         3707 c         3715 g         3172 t
ORIGIN      
        1 gttatgacgt gaaattttcg gtaaaacgcc ttcccttacc cttcctgcaa ccgtgacggc
       61 ggagccgaat gatagtgcgc atcggcctcg gcaaagcgtt tttgcatcgc cgctgacggc
      121 gctttaccca gcaaactaaa cactacaatc ccgatactgc cgaagataaa gcccggaata
      181 atttcgtaca gacccagcca gccgaactgt ttccagacga taaccgtcag cgcaccgatg
      241 atcatccccg ccagcgcacc gttgcgcgtc atgcgtgacc acatcaccga gaacagcacc
      301 accggaccaa acgccgcgcc aaagcctgcc cacgcgtagc tcactaagcc cagcacgcgg
      361 ttttccgggt ttgccgccag cgcaatcgcc accagcgcca ccaccagcac catcacacgc
      421 cctacccaca ccagctcttt ctggctggcc tgtttacgca gaaatgcttt gtatagatct
      481 tcggtaattg cactggaaca taccagcagc tggcaactta acgttgacat taccgccgcc
      541 agaatcgccg acagcaaaat cccggcaatc cacgggttaa acagaatttg cgccagttca
      601 ataaacacgc gttcagcgtt ctggtttacc gcacctgcta ccgccgggtg cccgttaaag
      661 taagcgatac caaagaaacc aacagccacc gcccctgcca ggcagaggat catccaggtc
      721 atactaatac gacgcgcatg gacaatgctg tggtgagaat ctgccgccat aaaacgcgcc
      781 aggatgtgcg gctgcccgaa gtaacccagc ccccaaccca tcagcgagat aatggcgaca
      841 aaattcaacc ctttgagcat atccacgttt tctatgctct tttgtttaat cacttccagc
      901 gagtcgccaa agccaccgac gctaatgatc acgataaccg gcgtcaggat cagggcaaaa
      961 atcatcagac tggcctgtac agtgtcagtc cagctcaccg cgaggaaacc gccaataaag
     1021 gtgtaaagga tcgtcgccgc agcccccgcc cacagagccg tttcgtagct catgccaaag
     1081 gtactttcaa acagacgcgc gcctgccaca atgcccgaag cgcaataaat ggtgaagaac
     1141 agcaaaataa ccagcgcgga gataatgcgc aaaatgcggc ttttatcttc aaagcgcccg
     1201 gtgaaataat ccggcaatgt taaggcgtta ttgttgtatt cggtatgcac acgcaaccgc
     1261 ccggccacca gcttccagtt gatccacgcg cctaatgtca ggccaatggc gatccagctt
     1321 tcggaaatcc cggaaagaaa aacagcgcca ggcaacccca ttaacagcca gccgctcata
     1381 tccgacgcac ccgccgataa tgccgtcacg aatggcccaa gactacgacc gcccagaata
     1441 tagtcatcga agtttttcgt tgatcgccag gcgataaacc caatcaatat catgccaaag
     1501 atatagacac aaaatgtcac caacatcggt gtgctaatag ccatctaaag tctccaaaaa
     1561 attattatcg gcaatgtcga aacttgccgt tatatctgcc accggaacgg ggtaacagag
     1621 tttatgtttt accagggcga ccgtatcctg ccggaagcgc tggttattca caatcgattt
     1681 aacacaccat ttacattaaa ttttagtgct cagcgacact atttttcatc aggttgcact
     1741 ctctcacatt ttttgcggtt gcacctttca aaaatgttaa ctgccgcaga gaaaaagtct
     1801 gagttatttt tttaatccct gtcatatcga tttcgtttat taacatttca ttcattttta
     1861 agcttgctac gcaggtcaca tttaacatgg ttgcacaaag ttgcaacatc atggatattt
     1921 cacgataacg ttaagttgca cctttcagaa caacaggagt aatggcatgg gaaccaccac
     1981 catgggggtt aagctggacg acgcgacgcg tgagcgtatt aagtctgctg cgacacgtat
     2041 cgatcgcaca ccacactggt taattaaaca ggcgattttt tcttatcttg aacaactgga
     2101 aaacagcgat actctcccgg agctacctgc gctgctttct ggcgcggcca atgagagcga
     2161 tgaagcaccg actccggcag aggaaccaca ccagcctttc ctcgactttg ccgaacaaat
     2221 attgccccag tcggtttccc gcgccgcgat caccgcggcc tatcgccgcc cggaaaccga
     2281 agcggtttct atgctgctgg aacaagctcg cctgccgcag ccagttgctg aacaggcgca
     2341 caaactggcg tatcaactgg ccgataaact gcgtaatcaa aaaaacgcca gtggtcgcgc
     2401 aggtatggtt caggggttat tgcaggagtt ttcgttgtca tcgcaggaag gcgtggcgct
     2461 gatgtgtctg gcggaagcgt tgttgcgtat tcccgacaaa gccacccgtg acgcgttaat
     2521 tcgcgacaaa atcagtaacg gtaactggca gtcacacatt ggtcgtagcc cgtccctgtt
     2581 tgttaatgcc gctacctggg ggctgctgtt taccggcaaa ctggtttcca cccataacga
     2641 agccagcctc tcccgctcgc tgaaccgcat tatcggtaag agcggtgaac cgctgatccg
     2701 caaaggtgtg gatatggcga tgcgcctgat gggcgagcag ttcgtcacgg gcgaaaccat
     2761 cgcggaagcg ttagccaatg cccgcaaact ggaagagaaa ggtttccgtt actcttacga
     2821 tatgctgggc gaagccgcac tgactgccgc agacgcacag gcgtatatgg tttcctatca
     2881 gcaggcgatt cacgccattg gtaaagcgtc taacggtcgt ggcatctatg aaggtccggg
     2941 catttcaatc aagctgtcgg cgctacatcc gcgttatagc cgcgcccagt atgaccgggt
     3001 aatggaagag ctttacccgc gtctgaaatc actcaccctg ctggcgcgtc agtacgatat
     3061 tggtattaac atcgatgccg aagaggccga tcgcctggag atctccctcg atctgctgga
     3121 aaaactctgt ttcgagcctg aactggcagg ctggaacggc atcggttttg ttattcaggc
     3181 ttatcaaaaa cgctgcccgt tggtgatcga ttacctgatt gatctcgcca cccgcagccg
     3241 tcgccgtctg atgattcgcc tggtgaaagg cgcgtactgg gatagtgaaa tcaagcgtgc
     3301 acagatggac ggccttgaag gttatccggt ttatacccgc aaggtgtata ccgacgtttc
     3361 ttatcttgcc tgtgcgaaaa aactgctggc ggtgccaaat ctaatctacc cgcagttcgc
     3421 gacgcacaac gcccatacgc tggcggcaat ttatcaactg gcgggtcaaa actactaccc
     3481 gggtcagtac gagttccagt gcctgcatgg tatgggcgag ccactgtatg agcaggtcac
     3541 cgggaaagtt gccgacggca aacttaaccg tccgtgtcgt atttatgctc cggtcggtac
     3601 acatgaaacg ctgctggcat atctggtgcg ccgcctgctg gaaaacggtg ctaacacctc
     3661 gtttgttaac cgtattgccg acacctcttt gccactggat gaactggtcg ccgatccagt
     3721 cactgctgta gaaaaactgg cgcaacagga agggcaaact ggattaccgc atccgaaaat
     3781 tcccctgccg cgcgatcttt acggtcacgg gcgcgacaac tcggcagggc tggatctcgc
     3841 taacgaacac cgtctggcct cgctctcttc agccctgctc aatagtgcac tgcaaaaatg
     3901 gcagtccttg ccaatgctgg aacaaccggt agcggcaggt gagatgtcgc ccgttattaa
     3961 ccctgcggaa ccgaaagata ttgtgggcta tgtgcgtgaa gccacgccgc gtgaagtaga
     4021 acaggcgctg gaaagtgcgg ttaataacgc gccaatctgg tttgccactc ctccggctga
     4081 acgcgcggca attttgcatc gcgctgccgt gctaatggaa agccagatgc agcaactgat
     4141 tggtattctg gtgcgtgaag ccggaaaaac cttcagtaac gccattgccg aagtgcgcga
     4201 agcggtcgat tttctccact actatgccgg acaggtgcgg gatgatttcg ctaacgaaac
     4261 ccaccgtcca ttagggcctg tggtatgtat cagtccgtgg aacttcccgc tggctatttt
     4321 caccggacag atcgctgccg cactggcggc aggtaacagc gtgctggcaa aaccggcaga
     4381 acaaacgccg ctgattgccg cgcaagggat cgccattttg ctggaagcgg gtgtaccgcc
     4441 aggcgtggtg caattgctgc caggtcgggg tgaaaccgtg ggcgcgcaac tgacgggtga
     4501 tgatcgcgtg cgcggggtga tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagcg
     4561 caatatcgcc agccgcctgg acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg
     4621 cggcatgaac gcgatgattg tcgattcttc agcactgacc gaacaagtcg taatagatgt
     4681 gctggcctcg gcgttcgaca gtgcaggtca gcgttgttcg gcactgcgcg tgctgtgcct
     4741 gcaagatgag attgccgacc acacgctgaa aatgctgcgc ggcgcaatgg ccgaatgccg
     4801 gatgggtaat ccgggtcgcc tgaccaccga tatcggtccg gtgattgata gcgaagcgaa
     4861 agccaatatc gagcgtcata ttcagaccat gcgtagtaaa ggccgtcagg tgttccaggc
     4921 ggtgcgggaa aacagcgaag atgcccgtga atggcaaagc ggcacctttg tcgctccgac
     4981 gctgatcgaa ctggatgact ttgccgaatt acaaaaagag gtctttggtc cggtgttgca
     5041 tgtggtgcgt tacaaccgta accagctccc agagctgatc gagcagatta acgcttccgg
     5101 ttatggtctg acgcttggcg tccatacgcg cattgatgaa accatcgccc aggtcactgg
     5161 ctcggcgcat gtcggtaatc tgtacgttaa ccgtaatatg gtgggcgcag tggttggggt
     5221 gcaaccgttc ggcggcgaag gattgtccgg caccgggccg aaagcaggcg gtccgctcta
     5281 tctctaccgt ctgctggcga atcgcccgga aagtgcgctg gcggtgacgc tcgcgcgtca
     5341 ggatgcggag tatccggtcg atgcgcagtt gaaagccgca ttgactcagc cgctaaatgc
     5401 actgcgggaa tgggcggcaa accgtccaga attgcaggcg ttatgtacgc aatatggcga
     5461 gttggcgcag gcaggaacac aacgattgtt gccagggccg acgggtgaac gcaacacctg
     5521 gacgctgctg ccgcgtgagc gcgtgttgtg tattgcggat gatgaacagg atgcgctgac
     5581 tcagctcgcc gccgtgctgg cggtgggtag ccaggtgctg tggccggata atgcgctgca
     5641 tcgtcagtta gtgaaggcat tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc
     5701 ggaaaatata accgctcaac catttgatgc ggtgatcttc cacggtgatt cggatcagct
     5761 tcgcgcattg tgtgaagccg ttgccgcgcg ggatggtgca attgtttcgg tgcagggttt
     5821 tgcccgtggc gaaagcaata tccttctgga acggctgtat atcgagcgtt cgctgagtgt
     5881 gaataccgct gccgctggcg gtaacgccag tttaatgacg atcggttaaa caggtagccg
     5941 gaggatgtta caccttcctc cggcaccatt agcgtggtcg aatcccctca ataataatcc
     6001 gctgcacgtt ttcaaccgtt tgattgaaaa atacctcatc gcgcaacgtc gcgcctgtca
     6061 ccgcctccac ctgaggggca aaatcggcgt aatgttgagt ggaagcccaa atcataaaaa
     6121 tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt aacccaaccg gcaatcagcg
     6181 ccgatttctc atcaattaat gccttcaaat cgcccgtcag ttcatccatt aacagcggtg
     6241 cacctgccag catctccata cagaacaggc gcgaagcctg cggataatcg cgggaaactt
     6301 ccagcttcag acggatgtac tctttgatcg ccgccagcgg ggcgaaatct tctcgaaacg
     6361 cttttaacgg tgccagccag atatcgagaa tctgccgcag tacggctata tacagcgcct
     6421 cttttgacgg aaagtaatac agcagattgg tttttgaaac acccgccaac tccgcgatct
     6481 gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa
     6541 tcgctttttt cttcgcgctc actgcgcgcg aacgtttacc cgttgttttc actgcgcctt
     6601 gcgtcatgcg ctctcccctc ttgtagattg gcaacaggat agcaaaaccc ccgcccggcc
     6661 tcgaccttct gcactctcat cgcgcacgaa tgcatgtttt atgtgcaact gttttgaccg
     6721 tttagtccac tttttttcag gcattttaga caacttaaca ttaacacatt aataacattc
     6781 actttttaaa aactggcatc cgctttgcaa acaagccatt acgcggctga aaaggatatg
     6841 caggatgcag cgccccgtct gactttcact ttacgagacg aagagaggtt gatgatgaaa
     6901 attggcgtat tcgtacctat tggcaacaac ggctggctca tttcgaccca cgcgccgcag
     6961 tacatgccga catttgaact gaataaagcc attgtgcaaa aagcggagca ctaccatttc
     7021 gatttcgccc tgtcgatgat caaactgcgt ggctttggcg gcaaaactga gttctgggat
     7081 cacaaccttg agtcgttcac cttgatggcg gggctggcag ccgtgacctc gcgcattcag
     7141 atatacgcga cagctgccac cttaacgtta cctccggcaa tcgtcgcccg tatggccgca
     7201 accatcgact ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg ctggcagaag
     7261 cccgagtatg agcagatggg gatctggcct ggcgatgact atttctcccg tcgttacgac
     7321 tatctcaccg aatatgttca ggtgctgcgc gacctgtggg gctcggggaa aagcgatttt
     7381 aaaggcgatt ttttcactat ggatgattgt cgcgtcagtc cgcaaccgag tgtccccatg
     7441 aaagtgatct gcgccgggca aagcgacgct ggcatggcgt tctccgcccg gtatgccgat
     7501 tttaacttct gtttcggcaa aggcgtaaat acacccacgg ctttcgcccc gaccgctgcg
     7561 cggatgaaac aggccgcaga gcaaaccgga cgcgacgttg gctcttatgt gttgtttatg
     7621 gtgattgccg acgaaaccga cgatgccgct cgtgcaaaat gggaacacta caaagcgggc
     7681 gcggatgaag aggcgttaag ctggctaacc gaacaaagtc agaaagatac ccgctcaggt
     7741 actgacacca acgtccgtca gatggccgat cccacttcgg cggtaaacat caatatgggg
     7801 acgttagtcg gttcttacgc cagtgtcgcg cgcatgttag atgaagtcgc aaacgtgcct
     7861 ggtgccgaag gcgtgctgtt gaccttcgat gattttctgt cgggaatcga aaccttcggc
     7921 gagcgcattc aaccactgat gcagtgccgc gcccatctcc ctgcgctgac tcaggaggtg
     7981 gcatgatgac gaccttaacc gctcgaccgg aagccattac cttcgatccg cagcaaagtg
     8041 cgctgatcgt ggtggatatg caaaacgctt atgccacgcc aggcggctac ttagatctcg
     8101 ccgggtttga tgtctcaacc actcgcccgg tcattgccaa cattcaaacc gccgtgaccg
     8161 cagcgcgagc ggcagggatg ctgatcatct ggtttcaaaa tggctgggat gaacagtatg
     8221 tcgaggctgg cggccccggc tcaccgaatt ttcataaatc gaacgccctg aaaaccatgc
     8281 gtaagcagcc gcagctgcag gggaaattgc tggcgaaagg ctcctgggat tatcaactgg
     8341 tggatgaact ggtgccgcag cctggcgata ttgtgctgcc gaagccgcgc tacagcggtt
     8401 tcttcaatac gccgctggac agcattttgc gcagccgtgg aatacgccat ctggttttca
     8461 ccggtatcgc taccaacgtc tgcgtcgaat cgacgctacg cgacggcttt tttctggagt
     8521 atttcggcgt ggtgctggaa gacgcaacac accaggcggg gccggaattt gcacagaaag
     8581 ctgcgttgtt caatatcgaa accttttttg gctgggtcag cgacgtcgaa acgttctgcg
     8641 acgcgctttc ttccacgtcc tttgctcgta tcgcttaagg agtttaacga tgccaaaatc
     8701 cgtaattatt cctgctggca gcagcgcacc gctggccccc ttcgttcccg gcacgctggc
     8761 tgatggcgtg gtgtatgtct ccggtacgct ggcttttgat caacataaca acgtgctgtt
     8821 tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa accatccgca aggtcatcga
     8881 gacggcgggt ggcacgatgg cggatgtgac tttcaacagc atctttatta ccgactggaa
     8941 aaattacgcc gcgattaacg aaatctacgc cgagtttttc ccgggtgaca aaccggcgcg
     9001 attctgcatt cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg ccacaattgc
     9061 gcatatcgcc aagtgaggcc gcgatgaaac tttcactctc acctccccct tatgctgatg
     9121 cgcccgtagt ggtgttgatt tcgggtcttg gcggtagcgg cagttactgg ttaccgcaac
     9181 tggcggtgct ggatcaggag tatcaggtag tctgttacga ccagcgcggc accggcaata
     9241 atcccgacac gctggcagaa gattacagta tcgcccagat ggcagcggaa ctgcatcagg
     9301 cgctggtagc cgcagggatt gagcgttacg cggtggtcgg ccatgcgctc ggtgcgctgg
     9361 tgggaatgca gctggcgctg gattatcccg cgtcggtaac tgtgctggtc agcgttaacg
     9421 gctggctacg aataaacacc catacgcgcc gctgttttca ggttcgcgaa cagttactac
     9481 atagcggcgg cgcgcaggca tgggtggaag cgcagccgtt gttcctctat cccgccgact
     9541 ggatggcggc ccgcgcacct cgccttgagg cagaagatgc gctggcactg gcgcattttc
     9601 agggcaaaaa taatttactg cgtcgactta acgccctcaa acgcgctgac tttagtcacc
     9661 atgcggatcg tatccgctgc ccggtgcaaa tcatctgcgc cagtgacgat ctgctggtgc
     9721 caacagcatg ttccagtgaa cttcatgccg ccctgcccga tagccagaaa atggtgatgc
     9781 gctatggcgg acacgcctgc aacgtgaccg atcccgaaac gtttaatgct ctgttactca
     9841 acgggcttgc cagcctgtta catcaccgtg aagccgctct gtaaggaatt gctatgaacg
     9901 aagccgttag cccaggtgcg cttagcaccc tgttcaccga tgcccgcact cacaacggct
     9961 ggcgggagcc ccccgtcagc gatgagacgt tacgggagat ttatgccctg atgaaatggg
    10021 ggccgacatc agctaactgt tctccggccc ggatcgtgtt tatccgcact gcagaaggaa
    10081 aagaacgtct gcgcccggcg ctttccagcg gcaatctgca aaaaaccctg accgcgcccg
    10141 tcaccgctat cgtcgcctgg gacagtgaat tttatgaacg gttaccacag ctgtttcctc
    10201 acggcgatgc ccgcagttgg tttacctcca gtccacaact tgccgaagaa acagcgtttc
    10261 gcaacagttc catgcaggcg gcctatctga tcgtcgcctg ccgtgcgctg gggctggata
    10321 ccggcccgat gtcgggcttt gaccgtcaat acgttgacga cgcctttttt gcgggcagca
    10381 cgctgaagag cgatctgctg attaatatcg gctatggcga ttccagcaaa ctttttgcgc
    10441 gcctgccacg tctgtccttt gaagaagcct gcgggctgtt gtaaggagca aacaatgaac
    10501 attgtcgatc aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc ggtcaatatc
    10561 atcactacgg acggtccagc cgggcgcgcc gggttcaccg ccagcgccgt ctgcagcgtg
    10621 accgacacgc cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt ctggccggta
    10681 ttcaatgaaa atcgaacgct gtgtgtaaat acacttagcg ccgggcagga gccgctttca
    10741 aacctttttg gcggcaaaac gcccatggaa caccgctttg ccgccgcccg ctggcagact
    10801 ggtgtgaccg gatgcccgca actggaaaag gcactggttt cgtttgactg ccgtatcagc
    10861 caggtggtga gcgtcggcac ccacgacatt ttgttttgcg ccatcgaagc gattcatcgt
    10921 cacgccacac cctacgggct ggtgtggttc gatcgcagtt atcacgcgct gatgcgccct
    10981 gcttgttaac ctccttaagg agacagctga tggcaatgtt cggttttcct cactggcagt
    11041 taaaatcgac ctctacagag agcggcgtgg tcgcgccgga cgaacgattg ccgtttgctc
    11101 aaacggcagt aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg gtgttaatgc
    11161 cgattctgat ggggctggat cccaatcttt ccattttaat gtcggggatc ggcacgttac
    11221 tgtttttctt tattaccggc gggcgcgtgc ccagttatct cggttccagc gcggcttttg
    11281 tcggcgtggt gattgccgcg acgggcttta atggtcaggg aattaatccg aacatcagca
    11341 tcgcgctggg cgggattatc gcctgtgggc tggtttatac cgtcattggc ctggtggtga
    11401 tgaaaatcgg tacgcgctgg attgaacgac taatgccgcc ggttgtgacg ggtgcggtag
    11461 tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa aaatgtatcg gcttcggcct
    11521 ttgatagctg gatggcggta atgacggtgc tgtgtattgg gctggtggcc gtatttaccc
    11581 gtggcatgat ccagcgatta ttgattctcg taggattaat tgtcgcctgt ctgctgtatg
    11641 gcgtgatgac caacgtttta gggctgggaa aagccgtcga ttttactctc gtcagccatg
    11701 ctgcctggtt cggtctgcca catttttcca ctcctgcttt taatggtcag gcaatgatgc
    11761 tgattgcgcc cgtggcggtg attctggtgg cggaaaactt aggtcatctc aaggcggtcg
    11821 ccgggatgac cggacgcaat atggacccgt atatggggcg ggcattcgta ggcgatgggc
    11881 tggcgacgat gctttctggc tctgtcggcg gtagcggcat aaccacctac gccgagaata
    11941 tcggtgtgat ggccgtgaca aaagtctact cgacgctggt gtttgttgcc gctgccgtca
    12001 tcgccatgct gttgggcttt tcgccgaaat ttggcgcgct gatccatact attccggctg
    12061 cggtgattgg tggtgcatca attgttgtgt tcgggctgat tgccgtcgca ggcgcacgga
    12121 tctgggtaca aaacagtgtg gatttaagcc agaacggtaa tttgattatg gtcgcagtaa
    12181 ccttagtgct gggtgcgggt gattttgctc tcacactggg cggttttacg ttgggaggga
    12241 tcggtacagc cacctttggc gcgattttac tcaatgcgtt gttaagcaga aggttggttg
    12301 acgttccacc gccagaagtt gttcatcaga agccataaac agaaaaccgc cggggctacc
    12361 ggcggtgatg gcgttttgac gagctacaga caaaaatgca tgcgtcattg attagcccgg
    12421 ctagttgtcg cttttaccgt gactgctctt gccacctttt ttacctgctt cagatgcgcg
    12481 ctgcgggtca tttttgaaat tacctccgct gtgctgtcca ccttttttac ctgcttctga
    12541 tgctctttcg cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga
    12601 cctccgtctg ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca
    12661 tattttcgct acgtggaact aagcttagtg aactgacgcg ggcagatccg cgtttggtaa
    12721 tatcctgcaa cagcttctta gcgagaatag atatcatctc caataattat ccataagccg
    12781 ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt
    12841 gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac
    12901 gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg
    12961 ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg
    13021 gctcgcgcag ttgctgaggg tgcaagcaaa gtggatggcg cagaagttgt cgttaagcgt
    13081 gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca
    13141 ccggttgcaa ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc
    13201 cgctttggta acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg
    13261 gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc
    13321 ggtcaggaac aaaccatcac atccacctgg acgacccttg cgcatcacgg catggtgatt
    13381 gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg
    13441 ccgtacggcg caaccaccat cgcaggcggt gacggttcac gccagcccag ccaggaagaa
    13501 ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc
    13561 taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg
    13621 aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg
    13681 ggtacgacga aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa gtgtt
//
LOCUS       DAOFSV010000122        13707 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.122, whole genome shotgun sequence.
ACCESSION   DAOFSV010000122 DAOFSV010000000
VERSION     DAOFSV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.122"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(35..808)
                     /locus_tag="QBH27_003627"
     CDS             complement(35..808)
                     /locus_tag="QBH27_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDQ7015895.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(810..1334)
                     /locus_tag="QBH27_003628"
     CDS             complement(810..1334)
                     /locus_tag="QBH27_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDQ7015896.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPS
                     GTYSLRQELIRTGD"
     gene            complement(1369..2565)
                     /locus_tag="QBH27_003629"
     CDS             complement(1369..2565)
                     /locus_tag="QBH27_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ7015897.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(2579..3241)
                     /locus_tag="QBH27_003630"
     CDS             complement(2579..3241)
                     /locus_tag="QBH27_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDQ7015898.1"
                     /translation="MDYMLNSTTSSGKRTGILAIGAHPDDIELGCGASLSYYSNEKVY
                     IISVVMTPGCCGAEYIDRHKESQEALNMLGCNKLIPLNFEDTRLYLQIKEMISSLEYI
                     IENETPQDVEITRVYTMNNTDRHQDHIAVYQASMVACRYIPQILGYETPSTCLSFLPQ
                     VFESVDEVHFNKKILALQKHKSQIHRRYIQPEYIIAQAKFRGQQVSHSMCEGFVVHKM
                     VL"
     gene            4280..5503
                     /locus_tag="QBH27_003631"
     CDS             4280..5503
                     /locus_tag="QBH27_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ7015899.1"
                     /translation="MGTIQKIRTDVNVLYAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            5488..6132
                     /locus_tag="QBH27_003632"
     CDS             5488..6132
                     /locus_tag="QBH27_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDQ7015900.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
     gene            complement(6503..8650)
                     /locus_tag="QBH27_003633"
     CDS             complement(6503..8650)
                     /locus_tag="QBH27_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ7015901.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWRFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
     gene            10322..10867
                     /gene="cobU"
                     /locus_tag="QBH27_003634"
     CDS             10322..10867
                     /gene="cobU"
                     /locus_tag="QBH27_003634"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDQ7015902.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            10864..11607
                     /gene="cobS"
                     /locus_tag="QBH27_003635"
     CDS             10864..11607
                     /gene="cobS"
                     /locus_tag="QBH27_003635"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDQ7015903.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            11619..12698
                     /gene="cobT"
                     /locus_tag="QBH27_003636"
     CDS             11619..12698
                     /gene="cobT"
                     /locus_tag="QBH27_003636"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDQ7015904.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            12760..13695
                     /gene="ldtA"
                     /locus_tag="QBH27_003637"
     CDS             12760..13695
                     /gene="ldtA"
                     /locus_tag="QBH27_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ7015905.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
BASE COUNT         3650 a         2912 c         3298 g         3847 t
ORIGIN      
        1 aactaccgaa taaaatatac tgatagactg ctgcctagaa tttccagatc aggtgggctg
       61 caagttgcag accgttataa tcatcggttg gggtgttgaa ccacacttta cctgccgtca
      121 acccgagatt aagttgctca gtcagcggtt gaattcgttg caaactgatg ctcttcatgc
      181 tgccgtagcg tgtttctggc gtccagtcgt aggtgtaagc acctgttccg cggcttagcc
      241 atagttgcgt ataaccagca ccgcgcgggt ccttcagacg cacggaaatc atattgctat
      301 aactactacc tgcatcgctg gagtcataat gggtatagcg gtaactggtg atcaccgggc
      361 cagtatagag tgatacgccg ccttgccagg cattgacttc gacatcatcg taatatttag
      421 tgtagcgata accggtcgtg aaaagcgttt ttggcaacag ggctaggtta atatcctgtc
      481 gaaaatcctg gcgggcaaag accggcgtat tatccgcaaa ggcaataccc gttcgggttg
      541 tcagccagtt attccatttg taccagactg caccctggcc gcgagtggca ttgaaatgtt
      601 cagtttcata atcacgacgc ccctgggaaa gattaaaaag cagcgttgcg ttttcaactt
      661 ttgctaccag ttcagcatac gctaaattac ggttgccatg ttcgccagaa taatcggtaa
      721 aatcgtagcc cgcggtaatg gaagttaacc cggcataagc gggcaatgca ctaaaaaaaa
      781 acggcactaa taacgtatgc tttatcattt taatctcctg tacggataag ttcttgtcgg
      841 agtgagtaag ttccactagg taaattacgt tctacaatca cgcgcgggcc aagttgtgtg
      901 ttcggagaaa taattcgccc aggcaaaata ataacttgta cgccaaggcg tgaacgcttc
      961 ccgatataac aacctaattt atcgcatccg gtagcgataa ttccctctgg agttcgaaca
     1021 gacacgggtt gttcatccag acgatgatta ctggtacgta cttgtgcgcc caaatatgcc
     1081 tggtttgcaa ctaccgagtc ggcaataaag cattgcggac caatcgttgc ttccgcttca
     1141 ataaccgcat ttttaatttc ggtggcaaaa ccaattttta cgccattgct gattattgtg
     1201 ccaggacgaa taaacgcata attacctatc aggcagtttg cgccaatcac taccggcccc
     1261 tgaataacgg caccgtggca aatacgggta ttcgcgccga taacaacctc accggcactt
     1321 tcatcaataa tcacctggtc tgcaatctga acatttttct ctttagcatt aagcttcaga
     1381 tagtttttca gttcctgtga cagaaggttg ggaataagcg gttgacgctt ccaccagtgc
     1441 ggaatagcgg gtgggtttgt cgcgctgagg ttcgcgtcca gtaaaaaagg caataagtcc
     1501 ataaataatc cagatggcat aagctaataa tacatacaca acggaaagcg gtgctaaagg
     1561 caccaacaac cagcaacgat gaaaccaggc gctaaatgca ccaataacac aaactacgcc
     1621 gacccagata agcgggaaca ttgccaacac cacaccatgt ggcccggtgg tgatgaattc
     1681 attaaaccag gtagtgagat aaataccaac gccataaagc acaaccaaca gcataggaaa
     1741 tatactgaag ataccgaagc ggctaaataa aagtctttta tgcaggcgca tacagaccgc
     1801 gtagcccaca atccaacgcc gccagcggcg ccactcctca cgcggactgt tacattcctg
     1861 tgggtatacg atgcagcgat tcgcctgccg aatacggtag ccgtttgcca ccaacgtcca
     1921 ggtgagatca aggtcttcga ctttagtacg atcagagaaa ccgaacttac gcaatacatc
     1981 agtacggaac atcccgcagg caccgctgat aataaacggt gcaccgccca gaaactgctg
     2041 tagcgtgcgc ttcataacaa tcatcggcaa ctttacggtg gcgcggatat gtggcagcag
     2101 acctgcccct tttaacgcgg tggagggaat tccgccaacg gcatcagcgc cgcgctcaat
     2161 ttctgccagc atatagccca ttccatcatt atcaggtgga acgaaagtat ctgcatcact
     2221 taaaaatact tgatcacacg tggcgtattt tagtccatgc attagtgcgc cacctttacc
     2281 ggtgtttttt tgcgtgacgg caacaaagcg gtcgccccat ttgcgtttga cttccgccat
     2341 gaccgcttcg gtattgtccg tggatccgtc gttaacgcaa attacccggc aaaaataagg
     2401 gttacgcagc agattatcca gtgactgcgc cagacacggg ccttcgttat aagctggtat
     2461 aatggcgtca atacagcctt tcttttgaca gggtttacgt cttagcgtac ttaaaaacca
     2521 tagcaacatt gctatggtca tgaagataaa taacaaagtt ttcatttcta ttactccgtt
     2581 acaacaccat cttatggaca acaaaacctt cacacataga atgactaacc tgttgtcctc
     2641 ggaatttggc ttgagcaatt atgtattctg gctgtatgta tcttcgatgg atctggcttt
     2701 tatgcttttg taatgctaat atttttttat taaaatggac ctcatctaca gattcaaaaa
     2761 cttgaggtaa aaatgacaga catgtacttg gtgtttcgta tcctagtatt tgaggaatat
     2821 aacgacaggc aaccattgat gcctgataaa cagcaatgtg atcctgatgc ctgtcagtat
     2881 tattcatggt gtaaactcga gtaatttcca catcctgggg cgtttcattc tcaattatgt
     2941 attccagaga tgatatcatc tcttttattt gcaaatataa tctggtatct tcaaaattta
     3001 gagggattag tttattgcat cctaacatat ttaatgcttc ttgtgattcc ttgtgacgat
     3061 caatatactc tgcgccacag caaccaggtg tcatgacaac agatataata tatacttttt
     3121 cattagaata atatgaaaga gatgcaccac atcctaattc tatatcatca ggatgggcac
     3181 caattgctaa aatgccggtt ctttttcccg aagatgttgt agaattcaac atgtaatcta
     3241 tccttttaga tatgccatat gaatccccat cataaattat cactatcata aaaattaatc
     3301 ttattaaata tactttatat ttactttttc agaggcagag aagcatcatt tatgcaacaa
     3361 gaagatcttc atttctgtta aaaaaacaga gtttatatgc tattacggat ttcagtcgta
     3421 gcaagttgaa ctgtgatcgc attaagacta atcttctggc ttgtaaaggc cttagttccg
     3481 tcatgactct cccatcaggc catagttgag caaaatcctg aaagaattat cacctattgt
     3541 atcttcagat acgggagttt ttgtccagta ttgttggaat aatcatgtga ctatattgtt
     3601 cttgtagttg gaacgtctta taaataaaac gaaaaggcag taagtttgtt gtatttattg
     3661 atatttttca ttcagttagt tggcaataat cagttatggt tgtattcaaa agtacagcta
     3721 gaacgaaatt aaaataacgg gagcatcgta catgacttga tatttcagag ggctaagtat
     3781 taatatatta ctacgatctg tcatcattga ctatcaatat attagttagt taaatatctc
     3841 tttgtgtcat tctggctagt atatactcat tatcatgaat gctgccatgc atagctaggt
     3901 catcaccaat taatgatatt ctctggataa tgattcaaga gtttaagttc attgtatata
     3961 taagtttgta ttcaaaggta ataaattcct tgtttattat ttctcatgaa aatagttaag
     4021 aaggagtgaa gagtctgttt ttttggtagg agagtcttta tttatgtgtt tgctattaat
     4081 gaatttttgt taaggtagcc tcatttgtca taattttgct aatatatgta tcgtcatatg
     4141 tcgtgtttta ctaaaaactt aactatcatg aactcgtgat agtaacaata tacccttaag
     4201 gtagaacttt ttcttgatga gaaggtttaa gtttgtagtt cagaatatta ctgccaagat
     4261 agtttatact aggatatgta tgggaaccat acagaagata agaacagatg ttaatgtatt
     4321 atatgcggcc attcaccgta ttgagtggtt gtttgagaca ttttcatctg tttgtttgtc
     4381 tttttctgga ggaaaagact ctactgtgct gctccatctt acggccgatg tggctcgcag
     4441 gaagaaacgt cgtttctctg tattattcat tgactgggaa gctcagtacc agtgcacaat
     4501 tgcacacatt ctgaagatga gggagatgta tcgggatgtg acggagactt tttactgggt
     4561 ggcactcccc ctgactacgg taaacggtgt ctctcagttt cagccggaat ggatatgctg
     4621 ggaaccaggt gtggagtggg ttcgtcagcc accagatgac gctattacag atatgtcgta
     4681 tttcccattt tatcggtatg ccatgacgtt tgaagaattt gttccggcat tttcttcctg
     4741 gtttgccggt aaccggtgtg gagtggcaat actgactggt gttcgtgctg atgaatcgct
     4801 caatcgcttt atgggactgg tgtctcagcg caaactgaga tatgcagatg ataaaccctg
     4861 gaccacagcg tcacctgaag ggttttatta caccttgtat ccgttgtatg actggaaagc
     4921 ccgtgatata tggatatata acgccagaac ccgagctatc tacaatcccc tgtatgacct
     4981 gatgtaccgt gccggcgtgc cgttacgcaa catgcgggtc tgtgagcctt ttggcccgga
     5041 acagcgtaag ggactgtggc tttaccatgt tctggagccg gaaacttggg ccaggatgtg
     5101 tgagcgggtg tcgggtgctg ccagcggggc gctttatgcc aatgagagcg gtgcctattt
     5161 tgccctgcgt aagcgtatca cgaagccacc tcatcatacc tggcgtagct atgcgatgtt
     5221 cctgctggat gtgatgccgg aaagaacggc agaacattac cgtaataaaa ttgctgtcta
     5281 cctgcgctgg tatcagacgc ggggcttccc ggatgacatc ccggatgaac aggagaatga
     5341 cctggggagc cgggatatcc cgtcttggcg acgtatctgt aagacactca taaagaatga
     5401 tttctggtgc cggaccctct ccttcagtcc gaacaaaccc cggcactatg aacgttatct
     5461 gcagcgtatg aaagaaagga ggaaggaatg ggggattctg tgacacctga ggtggaagta
     5521 ttgagcaaca tgattcgaca gtacttcagt caggaacgat ctgaagaaga gaccatacga
     5581 gcccttaatc atctgcgcag ggtgttgcat gaggtcagcc cttttgcaca ggagcctgtg
     5641 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa tcccaatgtc
     5701 atgtcttcca gtgaaaaaaa actgcttaaa cattcactgg agcaggatgg attcacgcag
     5761 cctgtggtgg tttcggaaga gaaggagcat tatctggtcg tggatggttt tcatcgtcag
     5821 ttgctgggca ggaaagcgga tacgagaaaa cgtctgaagg gttggctgcc agtgacctgt
     5881 attaatccgg aaagaaaggg acaggcatca cgtattgccg caaccatacg gcataaccgt
     5941 gccaggggaa aacaccagat aacgtcaatg tctgacattg tcagggacct ttcgcgtctg
     6001 ggctggacag atgagcgtat tggcacagag ttgggcatgg accaggatga agtgctgagg
     6061 ctgaaacaga ttagcgggct gacagagctg tttcaggaag agaatttcag cccttcatgg
     6121 acggttcgtt gatattgtaa aatatgatag ttggagtaag gggcaataga aggagaatat
     6181 ttatcccacc ttcttccgag tcgggataaa tcaaaaggta ttatccattg attcagtatc
     6241 tgggtgagag taaataatct aaaaatagta ttgcgttgtg aactgatatc acttttgaat
     6301 tacaaaggta taggctcagt aaccagcttt ataagtcatt gtcgtagcaa tgactataga
     6361 gtcatgctgg cgggaagaat aaatattcac ccccagatgg aaaacaacgt cgaaaaacct
     6421 tcaacaaaca caatacgttc gttagcctgc ccgcaaagcg ggcagatcag gacaaagaat
     6481 tccataacga ttgtttttgt catcagaaat tccatacaaa acctatagct gcattaaaac
     6541 ctgctgcagg aatagtttca tgtgctgtac ggtagcgttt gtctgtcagg ttattgagtg
     6601 ctaggttaat ccggtactga tcctcgttac cgaattctgt atttactgca aagttgagag
     6661 tggcccagcc cggaacattt gtttcggtac cgttactgtc atcttttgca ctagaggcag
     6721 cacgaataaa aacatcagag attatgttgg cctgacccat cacaagagta tgtttcagcc
     6781 ctatacgtcc gtttatcgct ggttctcctg tattagttgt ttttaatgtt gaagtttcat
     6841 attgccgacg aattaaattg ccactgatat atggcgagaa aacccagcca ttatattccg
     6901 cgcttatttc cagtccccat gtttttgccc gatcaatatt gtcataataa tagtagctac
     6961 tacgggagga gttggtgtta ccattgcaaa ctatactgcc atcacagatc agacttgcaa
     7021 tataatcttt agcttctgag tagtaaactg cgctgtcaat cagccactga ttaccattat
     7081 atcgtgcacc taattcaaag ttattggagt gttcagcctt aagatcagga tttccgtatg
     7141 tgacactgcc gcccgcagat gtctgcataa aaagctggga gagtgtggga aatacgtagc
     7201 cttgcgcgaa cgcagcgcgt aactccaagt tatcgaaacc tgaatagcgc aggcttgtag
     7261 atgttaccat ttcgtgatca ctcgcagact ctctggcaag agaggtatcg cttataatgc
     7321 ctgcggtata tgatactccg tcaccacgcg tcaactttga agaaagccag tattggcgaa
     7381 ctcccattgt ccatgtccag tgatcggcga atcgccagtc attttgtgcg aatagtgaga
     7441 ctgtactttg ctctgactca taataggagc gagttcgtgt ttccttatta atgaagccgg
     7501 tcagagattt gcttgaggtc atgccaccgg acctttggct gaccctgtct tgtttgtact
     7561 gtgcaccggt aacaagttca ttattagcag gcagcgaaaa gtgactctgc aatgtgaccg
     7621 cctgagtgta ttgcttatca tgggtgtcag tcttgttatg aacggtcaga gcctgaatca
     7681 tcggactggg aacaggctgt gtcgttttta cttcgttggc aaattggcgc tggatggtct
     7741 gctcatacgc gtcgaaatga atttttttta gatagtcacc gtccacgtct gtgtcataga
     7801 ataccccaac tttctctcgt tcaagtttag gtattttgac actaaatgcc tcatagcttc
     7861 cgtctggatc ctcatagtaa gtttgcgtcg cgagtctgta gcgatcaagc gagaggccaa
     7921 aacgatggtt tccggagtta taacccaacc atacaccctg actattgtta cgatagttgg
     7981 tattcggcag acgtccatcc ggcgtatcac gattgccctg atcagaataa ctaccgttga
     8041 tgcgataatc aaatccaccg atgctcccct ggaccgcgat tgattcttcc cagcctgctg
     8101 ttgcggaatt ataaacagct ttcacaactc cagatgcaag tttgtcacct ccctttttgg
     8161 ttatgaagtt aacaataccg ccaattgcct gtgaaccgta cagtacggaa tatggacctt
     8221 tcactacctc aacacgctcc agcgcagact catctatcaa cagtcccaca ccataattat
     8281 ctccggcgcg ctgataagtt acctcctgac catcaatgag aattaaaaca cgggaggatg
     8341 cttcgccacg aatgcggatt tgtttacggc ctgccaagga gttgtctgtt atctctactc
     8401 cggggatgtc ctgcaaatta tcggctatgg aggcattggt ggagttctgc aatgtttgtt
     8461 ggtcaataac ctgtatagtc gccgggcttt cccaaaggtt gcttgccgaa cgattcgtgg
     8521 tcacgacgag cgtctcttcg ttgctgattt ttgtttctga agaagttgcg gttgcagtag
     8581 aaactatccc agaaatgcat aatgacagag tggaaaggat ataaattcga caaggtatat
     8641 tatataacat ttcatccctc attaatacaa agtacaaatg attgctgtaa tgataattat
     8701 tatcatttat gttctcatgt atctgtgatt attgtcatct tcataaattg taaatatcaa
     8761 acatttttga ccttaatcat gttttttgat agatatttta cgactgaaca tatccgtcgt
     8821 ttcctctgac tcgtgtcagt cggtctctgg ttgtatccac ggaccgtact cttgacataa
     8881 gagtgcggtc cgtggaaaac ggagttgaaa aaatggtgtc tgaaagaagg taccatcatt
     8941 gcgatgacaa acactttggc gtggataatg taattaattg ctgaaattga ccgtatatgc
     9001 ggtaagactc atatcagtat caggtccggt taattgctga tactgacata aactttttac
     9061 aaaaggatga atgcagaagg ttactctaat ggccagagtg atgacaatga tggtcagacg
     9121 ttgacctgca gaaactgcat tttaattggt ctataaagtc tgcaagagga atcagggtca
     9181 gatcagtgag tcttatcgcg gcccgaagct atgactgaaa gaaggctgga tacagaattg
     9241 caacaatttg gcaatgtcag aacacagaag ctttgcaagc aggtgaaagg ttatgtctag
     9301 aaaaaggagt atatcagaat acatctactg atgtacaaaa cacaacaata gcttcatgat
     9361 atataaatac gtgatgacga gttgtactcc gcatggttat ttaacttctc agcaccacta
     9421 atgatcctga gcaacttcgt atttgggcca ttgccatgga gtagaaagct gtgtctgaga
     9481 atgcagaatt tgagaaaaga attcgctttc ttggagagct tctgaaaatg gcacgctaac
     9541 aacgctttgt taaaaaatgt gaatcacttg ccgaggctgc attttctatt cgaggagaat
     9601 gttgacgtag atattacaga aatctcagcg catcaggata aactgctacc ttaggtcaac
     9661 caccaaaaga aaatacaata cgtccagttt gtatgccttt aataacacat cttttaaatg
     9721 ttgaagaatt atcccgcctg ctgaagaacg ttgctctgag tgtgataaac ctttttattc
     9781 ttgatgatgt cagcgagaag atggaatata tccccgactg ggtcgtggtt acccggttaa
     9841 acatctggtg gcgctaataa atctggcaag tcacattctg ttgaatgctg tgacagtgcc
     9901 ttaccgagag agcgaaattg tggtgttcta gtgcatagtc gccaccattc cggataattt
     9961 tattgcgttg ttttacaact tcttttacag cgcagatctg tcgctgacgt tgaatcaata
    10021 gcctggattt cagaacacaa tgcccgtttg ctggttggtc cggcagtagc attaattcat
    10081 tatctgaatg aaatgtgtca atccacagaa accgctgtca atgacgcatt ttgtcggacg
    10141 ttaacacgag aaagtctggc tattccgtaa tgccatctta tttcgtaatg gattgagaat
    10201 gctggatttt taattatatt acgcgcagta ttaatcgcgt aaatgttttg cggtcagcaa
    10261 cggtggggga tccattgctc tgttcaatga ataccatcat acatttgaat caggagtcgg
    10321 tatgatgatt ttggtgacgg gaggcgcacg gagcgggaag agtcgccacg cagaggtgct
    10381 tattggggac tcttcacagg ttctgtatat cgctacctcg caaatccttg atgatgagat
    10441 ggctgcacgg atagaacatc atcggcaaag ccgcccggag cactggcgca cagtggagcg
    10501 ctggcaacat cttgatgaat taattcatgc agacattaac ccgaatgagg ctgtgttgct
    10561 ggaatgcgtt accacaatgg tgactaatct gttgtttgat tatggcggcg ataaagaccc
    10621 tgatgaatgg gattatcagg cgatggaaca ggcgattaat gctgagattc agtcgttgat
    10681 tgctgcctgc caacgttgcc ccgcaaaggt tgtattagtg actaacgaag tgggaatggg
    10741 gattgtgccg gagagtcgtc tggcacgaca ttttcgtgat attgccgggc gggtaaatca
    10801 gcagttggcc gctgcggcaa atgaagtatg gctggtggtt tcgggtattg gagtaaaaat
    10861 caaatgagta aattattttg ggcgatgctc tcatttatta cgcgtctgcc tgtaccgcgt
    10921 cgctggtccc agggactgga tttcgagcat tattctcgcg gtattattac ttttcctttg
    10981 attggattat tgcttggcgc gattagcggg ctggtcttca tggtgctgca ggcatggtgt
    11041 ggcgtaccac tggcggcact gtttagcgta cttgtgctgg cgctgatgac cgggggattc
    11101 catctggacg gcctcgctga tacctgtgat ggcgtatttt ctgcacgtag ccgcgatcgc
    11161 atgttggaaa tcatgcgtga tagccgttta ggcacccacg gcggtctggc attgattttt
    11221 gtggtactgg caaagattct ggtgttaagc gagttggctc tgcgtggcga accgatcctt
    11281 gcatcgctgg cggcagcatg tgcggtcagc cgcgggactg ccgcattatt gatgtatcgt
    11341 catcgttatg cgcgggaaga agggcttggc aatgtgttca ttggcaaaat tgatgggcga
    11401 caaacctgcg tcacactcgg cttagccgct atttttgctg ccgtattatt gccgggtatg
    11461 catggtgtgg ctgctatggt ggtgacgatg gtggcgattt tcatcctcgg tcagcttctc
    11521 aaacgtacgc tgggcgggca aacgggcgat acgctgggag cagccataga gtttggtgaa
    11581 ctggttttcc tgctggcact gctataaaga gacgtgacat gcaaatactt gccgatttac
    11641 tgaatacgat cccagccatc aattctgccg ctatgtcgcg tgcacaacgg catgttgacg
    11701 ggttactcaa acctgttggt agcctgggaa agctggaggc gcttgccata caactggcag
    11761 gaatgccggg gttgaatggc ataccgcatg tgggcaaaaa agcagtactg gttatgtgtg
    11821 ccgatcacgg cgtctgggag gaaggggtcg ctatttctcc aaaagaagtg acagccatac
    11881 aggctgaaaa tatgacccgt ggaacaaccg gcgtgtgtgt gctggcagca caagcgggcg
    11941 ctaacgtcca cgtaattgat gttggtattg atactgctga gcctatcccc gggcttatca
    12001 acatgcgtgt cgcacgaggt agcggcaata ttgcttcagc tccggcaatg agtcgccgtc
    12061 aggctgaaaa gttgcttttg gacgtcatat gttatacgcg ggagctggca aaaaacggtg
    12121 tcacgctgtt tggtgtaggt gaactgggga tggcaaacac gacaccggca gcggcaatag
    12181 tcagcacaat cactggccgg gatcctgaag aagtggttgg gattggcgca aacctgccga
    12241 cagataaact ggctaataaa attgatgttg tgcgtcgggc gattacgttg aatcaaccaa
    12301 atcctcagga tggtgttgat gtcctggcaa aagtgggtgg atttgatttg gtcggaatag
    12361 ctggagtgat gttaggtgct gcttcctgcg gtttacccgt gttgctggat ggatttcttt
    12421 cttatgctgc tgcgctcgca gcctgccaga tgtctcctgc aatcaaaccg tatctcattc
    12481 cttctcactt gtcggcagaa aaaggcgcgc gtatagcgct ctcgcatttg gggctggagc
    12541 cttatctcaa tatggatatg cgtttaggtg aggggagtgg tgcagctctg gcgatgtcca
    12601 tcatcgaagc tgcttgtgcg atatacaaca acatgggcga acttgctgcc agtaatattg
    12661 ttctaccggg gaatacgact tctgatttga acagctaaaa acgaaaagtc tgataaaagc
    12721 tatacttatc ggatagtatt cgcgacttag gaaagggaca tgatgcgtcg tgtaaatatt
    12781 ctttgctcat ttgctctgct ttttgccagc caaaatagcc tggcggtaac ttatccatta
    12841 cctccagagg gtagccgttt agtggggcag tcgcttactg taactgttcc tgatcacaat
    12901 actcagccgc tggagacttt tgccgcacaa tacgggcaag ggttaagtaa tatgctggaa
    12961 gcgaatccgg gcgctgatgt ttttttgccg aagcctggct cacaactcac cattccgcag
    13021 caactgattt tgcccgccac tgttcgtaaa gggattgtgg ttaacgtcgc tgaaatgcgt
    13081 ctttattact atccgccaga cagtaataca gtggaagtct ttcctattgg tatcggccag
    13141 gctgggcgag aaaccccgcg taactgggtg actaccgttg aacgtaaaca agaagcacca
    13201 acctggacgc caacgccgaa cacccggcgc gaatatgcga aacgagggga gagtttgccc
    13261 gcatttgttc ctgcggggcc cgataacccc atggggctgt acgcgattta tattggcaag
    13321 ttgtatgcca tccacggtac caatgccaat tttggtattg ggctccgcgt aagtcagggc
    13381 tgtattcgtc tgcgtaatga cgatatcaaa tatctgtttg ataatgttcc tgtaggcact
    13441 cgtgtacaaa ttattgatca gccagtgaaa tacacaacgg aaccggatgg ttcaaagtgg
    13501 ctggaagttc atgagccgct gtcgcgcaat cgtgcagaat atgagtctga ccgaaaagtg
    13561 ccattgccgg taaccccatc tttgcgggcg tttatcaacg ggcaagaagt tgatgtaaat
    13621 cgcgcaaatg ctgcgttgca acatcgatcg ggaatgcctg tgcaaattag ttctggttca
    13681 agacagatgt tttaagagcg ttggtaa
//
LOCUS       DAOFSV010000123        13594 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.123, whole genome shotgun sequence.
ACCESSION   DAOFSV010000123 DAOFSV010000000
VERSION     DAOFSV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.123"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..161
                     /locus_tag="QBH27_003638"
     CDS             <1..161
                     /locus_tag="QBH27_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ7015906.1"
                     /translation="DVDESLKGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKT
                     REYDDIRS"
     gene            389..685
                     /gene="ghoS"
                     /locus_tag="QBH27_003639"
     CDS             389..685
                     /gene="ghoS"
                     /locus_tag="QBH27_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDQ7015907.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            713..886
                     /gene="ghoT"
                     /locus_tag="QBH27_003640"
     CDS             713..886
                     /gene="ghoT"
                     /locus_tag="QBH27_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDQ7015908.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(1005..2522)
                     /gene="lysS"
                     /locus_tag="QBH27_003641"
     CDS             complement(1005..2522)
                     /gene="lysS"
                     /locus_tag="QBH27_003641"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDQ7015909.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2759..4216)
                     /gene="dtpC"
                     /locus_tag="QBH27_003642"
     CDS             complement(2759..4216)
                     /gene="dtpC"
                     /locus_tag="QBH27_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDQ7015910.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(4275..6422)
                     /gene="cadA"
                     /locus_tag="QBH27_003643"
     CDS             complement(4275..6422)
                     /gene="cadA"
                     /locus_tag="QBH27_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDQ7015911.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6502..7836)
                     /gene="cadB"
                     /locus_tag="QBH27_003644"
     CDS             complement(6502..7836)
                     /gene="cadB"
                     /locus_tag="QBH27_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDQ7015912.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(8202..9739)
                     /gene="cadC"
                     /locus_tag="QBH27_003645"
                     /pseudo
     CDS             complement(8202..9739)
                     /gene="cadC"
                     /locus_tag="QBH27_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            complement(10476..11330)
                     /locus_tag="QBH27_003646"
     CDS             complement(10476..11330)
                     /locus_tag="QBH27_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDQ7015913.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSICRILAVRKSTLLQIDTLIRQ
                     LADISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERNEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSACSRV"
     gene            complement(11415..11612)
                     /locus_tag="QBH27_003647"
     CDS             complement(11415..11612)
                     /locus_tag="QBH27_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015914.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            complement(11624..12112)
                     /locus_tag="QBH27_003648"
     CDS             complement(11624..12112)
                     /locus_tag="QBH27_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015915.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVNLAELINHVAENGYS
                     LRVVEESDQQSTCTPPPFTALAGIRCSTAHITEKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(12109..12486)
                     /locus_tag="QBH27_003649"
     CDS             complement(12109..12486)
                     /locus_tag="QBH27_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDQ7015916.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNDITLGKHPEAKQ"
     gene            complement(12576..12944)
                     /locus_tag="QBH27_003650"
     CDS             complement(12576..12944)
                     /locus_tag="QBH27_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDQ7015917.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLGQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGCVYLAVYPTPETKK"
     gene            complement(13023..13244)
                     /locus_tag="QBH27_003651"
     CDS             complement(13023..13244)
                     /locus_tag="QBH27_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDQ7015918.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(13313..>13594)
                     /locus_tag="QBH27_003652"
     CDS             complement(13313..>13594)
                     /locus_tag="QBH27_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015919.1"
                     /translation="NQNKLIAGETLFTGTINRTEVHPREVIKRALYHNAAAVVLAHNH
                     PSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         3697 a         3154 c         3213 g         3530 t
ORIGIN      
        1 ccgatgtcga tgaaagcctg aaaggacaag ggattggtaa acagctggtt gcgaaagtcg
       61 tggaaaaaat gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg
      121 aatttgataa aacacgggag tatgatgata ttcgcagttg atgggagagt acagagtcac
      181 gatatttttc atgctctccg cggtgtgatg caggagagca tctgaagggt aggagatgca
      241 caaagaatgg tcagagagag cgtttttttg tcccaagtca tcccctttac tgagcaaaaa
      301 aaaaagaata tctcctatat gagaatcatc attcggggtt aataagtttt gcgtccccag
      361 agcgttcaat attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt
      421 tgtttctttt gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat
      481 gtatgatatg aatttctcct ctatcgtggc tgatgaatat gggataccac gacaattgaa
      541 tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag
      601 gctcaaatgc ttagacttac cggatattga ttttgacctc agcattatga cagttgatga
      661 ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact
      721 attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg
      781 gtttatctca catgagaaaa cacatattcg tttacttagt gcatttctgg tcggaataac
      841 ctggccaatg agtttgcctg tggcattact tttttctctc ttttaggagc tgtagttgag
      901 tggacccgct ggttatcttg aaacgaagcg tctctcctca aaactggatt tgcggaatga
      961 cgaacgaagc agtcgcctga ctacttcgtt cattaaagtg aaatttattt ctgtgggcgc
     1021 atcgccggga agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt
     1081 cggtcgatac caatacccag accagcggtt ggcggcagac catattccag cgcagtcacg
     1141 taatcttcgt catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc
     1201 tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accaatttca
     1261 cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc
     1321 gccagcgggg acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct
     1381 tctgccactt catcaaagat ctccgtaaca atacgcccca gtccccagct tttctctacc
     1441 gtaataccga tagattcagc taatgcttta gcagcatcaa aattatccag atccgccata
     1501 tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg
     1561 ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagtac ctcttgtgcc
     1621 agggtgcgga acagtgactc cgtcagttca atcaaatcat ggtaatccgc atatgccatg
     1681 tagagttcca tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag
     1741 ttacggttga tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc
     1801 tccggcgcga tacgcaggta catatctaaa tccagagcgt tatgatgggt aataaacggg
     1861 cgagcagatg ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg
     1921 cgcgcgacca tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga
     1981 cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc
     2041 tgcagaccat ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcagctca
     2101 gtacagtgaa tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata
     2161 atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct
     2221 cttgcaacgt acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc
     2281 cccataatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc
     2341 agttcctggt tatccttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg
     2401 cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga
     2461 tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc tcgttcagac
     2521 attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca
     2581 ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc
     2641 tcaagttgat atcactaata aataaccatc aaaatggttt aaatgactta ttaatctcga
     2701 tttactagtg tgataaactg aaagttaatt tattcataac caattgtttt tacgacagtt
     2761 aatcgttgct ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg gtagcaaagg
     2821 ccaataccac gatgatcgcg acacaagcca acgtccactc tcccatctga gaaaagaatc
     2881 gctggtaagc ggcaattgcc ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga
     2941 cgcctgccag ccagttggcg accgcgcctg ttgccagcat ataaataccg gttaatacgc
     3001 cagacatttt cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag agttcagcaa
     3061 agcccattag cgccagcccg gatatcatca cgcccattga cgcttgaccg ccagccgctg
     3121 catgtcgggc atcaaatgcc agcaacataa agccacaagc catcagcagt aagccaaagg
     3181 caaacttcag ccagacgcgc aatgctgaat tgccgcggct ttctggcgac gccagccagg
     3241 ccagaacaac cccagcgagc atcaccgcaa tagcattcac cgactggaat agtgctgtag
     3301 gtacttcaat attgaatgcc tgacgattca caaagcgatc gataaacaag ctgatggtac
     3361 tgccgccctg ttgtgccagt acccagaaca acgtcccgac aaacatcaac aatacaattt
     3421 gccaaagagc acggcggtgt tcggggaatt tgatcatcat gcgggcaatg atttgtgctg
     3481 caatgaggca aacgatcgcc agcaaatatc cggaccagtc gttctccagc agcagggtaa
     3541 aaaatactgg ggctaaacag agcatcacca ctaaccagct ccatactggt aaggcaaatt
     3601 tgacgctagt gagcgctttt ttatccatac tacgcgtgga ctggaaatga cgatgaccac
     3661 ttaagaaaat caacaaaccg ataaacatgc cgcctcccgc aagggcaaag ccaacatgcc
     3721 atccatacca ctgagcagcc aggccgcagg cgatgggcgc ggcgatagaa ccgatattgc
     3781 ccgcagcata cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg tcgtagagct
     3841 cgccaagcaa acagctgatg tttgatttga ataaaccgta gccacaaata atgattgcca
     3901 gcgccagata caggctaaag gttgaatttg tatcaatgcc cagcaccaca tggccaaggg
     3961 tcattaacag cgcgccggca atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc
     4021 agccgccgag aataggggta acgtaaacca gagaagcata tgcgctgaac aggctgatgg
     4081 catggttatc atcaaaacca agctgatggg tgagatagag aatgagtaag gcacgcatgc
     4141 cgtaaaaact gaagtactcc cagatttgga tcgccacgat atagtatatc gcgcgcggct
     4201 gtgagggtgt tttcatgtgt tctccttatg agcaaaaaag ggaagtggca agccacttcc
     4261 cttgtacgag ctaattattt tttgctttct tctttcaata ccttaacggt atagcggcca
     4321 tcagcctgac ggtatgcacc gtgaatatcg gtttcaaagc ccggatagtg agcgccgatt
     4381 tcacacagca tctgcaggaa ctccagaacc ggacggcttt cttcggtgat catttcaccc
     4441 ggcattacca gaggaactcc cggcggatac ggaaggatca tattggcgtt aatacgacct
     4501 accatttcgt cgaggtaaac ttcttcggtc ataccgtgca gctctttctg gaacgcagca
     4561 tacggagtca ttaccatagt cggcagaact tcaaatgcgc gatacatcag atccggcaga
     4621 ttgtggtgaa caatcagttt gtggatattc tgagccagtt cctgaatacg catgttttca
     4681 tagaattcag gatcttcacg atacagagac ggcagcatgt ttttcacacg caggttcaga
     4741 tcgaacgcac gtttgaagtc agtcagagca cgcagcaggc tcagtgcttt ggtcttatcg
     4801 ataccgatgc tgaacaggaa cagcaggtta tacggaccgg ttttctcaac aacgatgcca
     4861 tgttcgtcga ggtatttcgc cacgatgctg gccggaatac caaagtcgct catggtgccg
     4921 tctttttcca tccccggagt cagcagggtg actttgatcg ggtcaagata catgtgctcg
     4981 ttatcgatgt ttttgaagcc gtgccaggtg ctgtcagaac gcagcggcca gcattcagtc
     5041 gtatcgatat gatccggctg ccatacatca aagaaccagc catcagattc cgttctcaga
     5101 cgtttgatct ctttacggaa tttgatcgca cgttcaatgg aaccattgat cagacgttta
     5161 cctgcattgc ctttcatcat cgctgcagcg gtttcagtgg acgccacgat accgtagtgc
     5221 ggagaggtgg tggtgtgcat catgtaggct tcgttaaagg tttcttcgtt tacgtcacct
     5281 ttaacgtgga tcatggaagc ctgagagaac gccgccagca gtttgtgagt agactgggtt
     5341 tcgtaaatca ctttcccttc tacacggcca ccgctcatac cgcatttacc ttcgtaaatc
     5401 ggtgagaagt tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt
     5461 gttttcttga tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca
     5521 gcatgtaccg gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc
     5581 tggaattcac tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa
     5641 tagattggcg taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg
     5701 tcaatcagaa tggtgctgcc tgccggagca gagtacatac caacaatttt gttcgcagtg
     5761 gaagtaccgt tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt
     5821 tctgcttctt tgtgtggacc actgtgatcc agcagagaac ccagttcaga tactgaaatg
     5881 gaaatatcag atttcatggt gttcggacca aagaaatcat agaacaggct acctaccggg
     5941 cttttctgga atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca
     6001 cgaacatatt taaacagtgc tttggtcagc ggaggcagaa tagtgttgat atattcgtca
     6061 gtggtctgct tgatcttgtt agcaatatca tcagcagcac ccagcgcata ttcaaagaag
     6121 ctaatctgta aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac
     6181 gcgtacaacg gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat
     6241 ttatcccagt caaaaataac accgcacaga cgcgcattgt tttcgatcag ttttaataag
     6301 tcgtcacggt cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt
     6361 tcacggatgg gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc
     6421 atagtcatat ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg
     6481 agcctcggaa aatactttta attaatgtgc gttagacgct gtgtggttat ccattgagtg
     6541 gctctggcgc tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa
     6601 ggtacctgcc agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacgcaacc
     6661 cagtacagag cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag
     6721 gtcaacgcaa gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc
     6781 accgaacagg tcagaggctt taccaccggc agagttcatc agagtgatga ggatcatcag
     6841 ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt tgctgtcgac
     6901 ttcaccataa actttcggga agttaccgtc gttagcggca cgtacacctg cctggcctac
     6961 caacatcatc caggagccca gagaagtcag acacgcaaag gcggtgaatg cagaaaccag
     7021 tggcgcagcc cagttaccga ggatagttga agcactgatt gcaaacggag caccggaagc
     7081 cgccattaca gaagacggat acataccgga aagcacctga gtcgcagcga tgtaaacaat
     7141 acctgctaaa ccagtaccca gcatggttgc cagcggaacg gtacgtttcg ggtttttaac
     7201 cataccagta cttactgctg cggattcaac acccacgaag gcccacaggc agagcagaat
     7261 acttttaatg atcgcatgac catcagtggt atccgcagta ttccagttag ctgcataagt
     7321 tgctgcatca aaccaatgcc agccaacaat agcagtcatc accacaggaa taagaaccag
     7381 caccagacca atagtggtta aacggcttac ccaggtaccg ccgagcatat ttacaaaggt
     7441 aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat ttaatactgg
     7501 gaagaaggtg gaaagataag atacagcggt aataccaatt gccaggttac caatccagtt
     7561 agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggggaaattt ctccggcata
     7621 cgcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata catatgccag
     7681 cgacattgca ccaataatag agataatcca accccagata gcaataccac cgatacttgc
     7741 taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa caacaccggt
     7801 acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat ttcatttttg
     7861 aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac tggcgctaag
     7921 attactcacg aaaaaaggat taatcctaaa gattaggtga aataacacaa aagtttctgt
     7981 aagtgagaac ttgaggtttt ttattaacac atcaggatcg caagttgata tcatgaaaag
     8041 ataaacattc aatgtttaca atgcattgcg tgacattctc tggttaaatt tatgtaataa
     8101 aaattatgcg gcaaataaat tgccgcaaca tattatacca acgggaacat acaaaaactc
     8161 aacaacaaat attcccgagc atgaatcaac ccgggagtta cttattctga agcgagaaat
     8221 ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt ttcaatccag
     8281 taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc agctgcttcc
     8341 cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag ccaggacatt
     8401 tcaagatcaa tgccggtatt tatcgcctga taagactcat ctgttttacc ttttaccagg
     8461 gcactgaccg cttttatttg atatataatg gacaggttgt tcaattccgg cagtgtaaca
     8521 atgttatcta tttctgtgtt cagtgctgct aattgttttt cgtctaaagg atgttgagaa
     8581 tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc tggggatgat
     8641 tgaacaatct cacctaataa ttcactggca cgatccaatg atttatcatc accatgcagt
     8701 aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgcgg caaaatttgc
     8761 tggagcatct cctgcattcg ttgtggccac ggttggttta acgcttttga taaactctgg
     8821 agcaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg tttatccagc
     8881 atggttgaac catctgcatg gtctaccaat tttaccgaca taaagcattg ttgagcacgg
     8941 tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct cggttcatca
     9001 atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag tgatgtcgcc
     9061 accaaatcac ccacgcctat cgcgtaagag agctgatacg gagaactcca gctgttacaa
     9121 ctcttattaa ccatattaat gtcaatatcg cgtggattga gcaaaatgcg cgatttactc
     9181 ataggaagac gtgtttcaag acttgaaaac gctaccagtg cgacacagat acctaacgac
     9241 aacaggaaaa aaaccatacc caaaaggtag tgaatcgttt gcttttaact ggggattgtt
     9301 caggtggcgt tgtggtgttt tgaatgttaa gactgtggga gggagaatct gtggcaggaa
     9361 ccgcctctgg tataggggga ggcgaagata gcattatttc ctctccctct tcttcgctgt
     9421 accagataac cggcaccatt aatttatagc cgcgctttgg tacagtagcg atatagacag
     9481 gactatcttc atcattatct tttaatgact tacgtagttc tgagatactc tgcgtcacaa
     9541 cgtgattggt gacaatactt ctcttccaga cattatcgat aagttcatct ctgctaagta
     9601 cttcgccact gtgttgagca aagaaaacca gaagatcgat taatctcggc tcaagggtaa
     9661 gttgacgccc attgcggcta atttggttta tggacggagt aacaagccat tcgccaacgc
     9721 gaactacagg ttgttgcata atagaaactc attcgaaaag ggaatgatgc aatgataatt
     9781 gccataacct attttttcca tctatagatg ggtttattta catattattg gtgaatgcaa
     9841 gacgttattt ttactcgcca taaacttctt gattacatag tattacgaaa ggattttact
     9901 gagaaccaga agtaattttc cttaccatca aaattcatca tctttgccaa agaaaaatgt
     9961 tcagaaaata atccatgaaa aattgtccgg agcgcttact attttaatgg attgttagtc
    10021 tttgcatgag caaacggact gatacatttc tctttgttct cattcagaaa atctcatcag
    10081 ttggcgttct gcccgatgtt gtgctttatg gatgcattac gctcctgatg acgtcatttg
    10141 acgttcaaca gcatcacggg gccgcacgac atttcacgtc agtcagcgct atagctcaga
    10201 aacaaatttt cccgaattgg gatatgcccg caaattgctg gtgatgtggg gagaatctgg
    10261 ttgagttcgg tagaattgat ttggcggcag gacgcttaaa tcgtggcgtc ctgaaacgaa
    10321 aaatggacct ccgtggaggt ccgtttatat gaatttggtg ccggactcgg aatcgaaaat
    10381 ttcttttatc atattgatat taatataatt tatttggttt tattaaaaat ataccctcaa
    10441 ttatacccgc agtccctttt gatatcgaaa gggaatcaca ctcgggagca ggctgagagc
    10501 acagaaggaa agtacctggc tattatatcg ttaattctgt caatcagttc cagccttttg
    10561 aaagtgatgt gtgcagatcc cttcttaaag tatttaatga tgaacatctc gtcttcattg
    10621 cgctctttac cctgaacgga ctggatgtga ccatccagac gaatagtgat gttttcccgg
    10681 ttgtcgggga tcggtttgcc gctgaacaga tgtagcattc tttccaggtc gacgagtcgg
    10741 tcagtctgtt gcccggtgat aagatgaaac ccccatcggt cccacctcac taggttgttg
    10801 acgataattt tactgccaaa tttgcagggg caattggttt tgtaattcca gttcagtccc
    10861 ctgaagacgt tgatcacccc tcgctcaaac acctcatcct tattctggtg cagttgttca
    10921 aatgtgctca gaatgttcgc ttcactgatt tccggaaaat tatcgtattc cagtgaccgg
    10981 taccatgtat cacgggcctg cgcatccatt aatgacagca tcccggaacg ttgcatcagg
    11041 tcacgccaga tttcgcgatc gagattgcgg gtgatggctt tcattgctgt ttcaggttta
    11101 tccatcagcc agcagccaca gcgaaaatcc tgtttcatgg cccagtcccg ggcggtttta
    11161 cctccaatgc tttccgtcag aactgagatg tcagccagtt gccggataag tgtgtcgatt
    11221 tgcagcagtg tactttttcg tacagccaga atgcggcaga tactggttga gcagataacg
    11281 tccgtatgcc cgttgaccac ttccggacgg gaggctgtga tatcagacat aatcgatatt
    11341 cctttttgca gataaatgaa aacgccagcc ctgttcaggc tggcgtcagt ggaatgaagt
    11401 ctggtgagta atatttaccg gttctggtgc agaagctgca gatgacgaag agtacggaca
    11461 tcctcccggg cccgttcgat acagggcaac agtgctgccg aatccgtttt atcctcatcg
    11521 ttgtcaaaga taattcccga ttcgcagtcg atattgtcct gcagccacac aatcagaata
    11581 tccagtgctg tttccgtggt taatgatttc atgttgttcc tggttaccag tcgaaagtgg
    11641 gcaggtcagg cagacattca gcgctggcat ccagatgaat gagactgacg ccataacgcc
    11701 ggataagtgt gacaaccaga cggcggaacg tttttgacag tcccaggcgt ttaagccgca
    11761 gaaccgggta tgaccacgca tcggtacgta acagatatcc gctacctgta aaatgaatcc
    11821 attctgattc gccgaagtca ttcgtctgat gtgacagcga gtacagccag gcgttgtcct
    11881 tttccgtgat atgtgcggta ctgcagcgta tgccggcaag ggccgtaaac ggtggtggtg
    11941 tgcaggttga ctgttggtcg gattcctcca ccacgcggag tgaataaccg ttttcagcga
    12001 catggttaat cagttcagcg agattcacac catcgacgtc aacgacaatg cgccccatgt
    12061 tcagtgcctg cacgttaatg ctgtcggctt ccagcgtcag ggccagtttc attgtttcgc
    12121 ctccggatgc ttacccaggg taatgtcatt taccgttctg tagttgtcgc gggtcatcag
    12181 gccggttgcc cggcgtgccc ggaggatatc aatactgttt attaactgag aacgggtaca
    12241 ggcactgaat tccggctggt cggtacgcac cagtgcgtat ttttcaacga gaaagttcac
    12301 cgcgtcacac agtgaaatgc ctgcctcaat atgctgctca atcacacgtt catcggcgaa
    12361 cggtgtgtca ttcagggtaa ggccgtaatg ctggcccagt agtcgggaga gcagagtctg
    12421 ccagatggtg acgggagacg ggcagctcga tgcctcccgt acgtgtgtat cgggtaatgt
    12481 tttcatactg aaggttttcc tgatatgcag agatgaaaaa tggaaaagtg gcgtggtgaa
    12541 agcaccaggc cggagcagaa ggttattccg gggggttact ttttcgtttc gggtgtcgga
    12601 taaacagcca gataaacgca accacagctg ccgagggtat cggcttcgca ggtcagccct
    12661 tttgcataca gcgtgacggt atgctgatgg cggggattca gttcaccgct ggtgagcatg
    12721 agttccagtt gtttcatcag cagcggaaag gcctggccca gatggtatgc atccgcgtcg
    12781 ctgaaccggc ctctgatacc ggcacggtcg gcaaggtaat gcaaccggtt accctcctgc
    12841 accagacgtg ccccgaaaca gggcgtcacg gtgcagggca gcccccacca ggggcggtcg
    12901 tgattgtcgt cgggatgcgt tgtcccgggc agtgtgtctg acacgataaa gtcctcacag
    12961 aaatcagtta agaatggttc gatggtggcg ataattcggc tcatcagaat gcccttcctg
    13021 actcagttca gggtgacgct catcagccgg acatacgggc caaaactgtc tttacggcgt
    13081 tctgcaaaca cagccagcac accgggaata tcctgcactt cacgaccggt atacgcttca
    13141 gtgctgccgt gccagcggta tttgccggta cagaacggaa aaagacggga tgccggatgt
    13201 tgttggtgaa tacgcatggc ttcaccacgg gtgatgattt tcatagtgtg atacctctga
    13261 agacagaaga taaaagcgaa aacaggtatg atgtggttgt aacggtgacg ggttaaagca
    13321 gaccgtgttc cgcaaaggag aaaacctggc tgccaccgac tatcagatgg tccggcaccc
    13381 ggatatccac caggcccagt gcctgtacca gacgttccgt gataaggcgg tctgccttgc
    13441 tgggtgtgac ttcaccggac gggtgattgt gcgccagtac cacggcagcg gcattgtggt
    13501 acagggcgcg tttaatcact tcccggggat ggacttccgt gcggttgatg gtgccggtga
    13561 agagggtttc accggcaatc agcttattct ggtt
//
LOCUS       DAOFSV010000124        13454 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.124, whole genome shotgun sequence.
ACCESSION   DAOFSV010000124 DAOFSV010000000
VERSION     DAOFSV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.124"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(218..1411)
                     /locus_tag="QBH27_003653"
     CDS             complement(218..1411)
                     /locus_tag="QBH27_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDQ7015920.1"
                     /translation="MKKIVLPVALALLGMNAGAYAADVSIDILSATVKDKRIEGASVT
                     LQRNGAQSVSGITNTSGSISLGTSFADNKDALLIVKKEGYSNLVVKCPCAGMTYAISP
                     VMTNLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGPETVT
                     ISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIW
                     TVFKLNPNGEFEDINSVTSANFNGTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQL
                     ARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWAN
                     RKAISLASGVNAATTRANSYYNIAKIYETSGQNAEALQHYQLAYTEKNKPSYEEAIAR
                     VKTKM"
     gene            complement(1609..4242)
                     /gene="mngB"
                     /locus_tag="QBH27_003654"
     CDS             complement(1609..4242)
                     /gene="mngB"
                     /locus_tag="QBH27_003654"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDQ7015921.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEGGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLAILKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFDYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRGSQFNLRDGRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQISQIVPSMGYRTLYIEANQLGNVVTPKSKTEGILENAFWQIALNEDGSLRLV
                     DKDSGVRYDRVFQIEEGSDDGDEYDYSPAKEEWAITSANAKPQYDIIHEAWQSRAIIR
                     YEIAVPRNLSERRAKQCSGRVGVETVITLSHNSRRIDADINLDNQADDHRIRVLIPTP
                     FNTDVVLADTQFGSLTRPVKDCAMNVWQQEGWKEAPVPVWNMLNYAVLQEGRNGIAVF
                     SEGLREFEVIGEENKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVSDSQLRGSFSC
                     RLSLFSYIDTPVTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAKFNVPESYSLLKMPP
                     VGCLISALKKAEDRQELILRLFNPAELTSCDATVAFSRKVMTCTETMMNERFNTEKNE
                     VLKELALFLPGQSRTFSYRIV"
     gene            complement(4260..6176)
                     /gene="mngA"
                     /locus_tag="QBH27_003655"
     CDS             complement(4260..6176)
                     /gene="mngA"
                     /locus_tag="QBH27_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDQ7015922.1"
                     /translation="MNLTTLTHRDALCLNVRFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYCRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMKLLTALTTRLADDELRARIQSATTPDELLSALDDKGDAQHTASF
                     SYAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNIYVEKQGANGIEERLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGNNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRHH
                     AVKHGNYLTDGVMP"
     gene            6345..7067
                     /gene="mngR"
                     /locus_tag="QBH27_003656"
     CDS             6345..7067
                     /gene="mngR"
                     /locus_tag="QBH27_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDQ7015923.1"
                     /translation="MGHKPLYRQIADRIREQITCGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVETHSEVLIF
                     EVIPADNFLQQQLQISEQDRVWHVKRVRYRKQKPVALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGQVFEY
                     SRNAFNTDDYKFTLIAQRKSPR"
     gene            complement(7171..8040)
                     /gene="sucD"
                     /locus_tag="QBH27_003657"
     CDS             complement(7171..8040)
                     /gene="sucD"
                     /locus_tag="QBH27_003657"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDQ7015924.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(8040..9206)
                     /gene="sucC"
                     /locus_tag="QBH27_003658"
     CDS             complement(8040..9206)
                     /gene="sucC"
                     /locus_tag="QBH27_003658"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDQ7015925.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(9300..10517)
                     /gene="odhB"
                     /locus_tag="QBH27_003659"
     CDS             complement(9300..10517)
                     /gene="odhB"
                     /locus_tag="QBH27_003659"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDQ7015926.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDVVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(10532..13333)
                     /gene="sucA"
                     /locus_tag="QBH27_003660"
     CDS             complement(10532..13333)
                     /gene="sucA"
                     /locus_tag="QBH27_003660"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDQ7015927.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAISEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
BASE COUNT         3221 a         3619 c         3227 g         3387 t
ORIGIN      
        1 tatgaaatgg catcgtgaag aggcgccagc caattataaa gatcttgctc ctcaaaaaat
       61 gaaacaactc agcctgagat aatatttttg accgaagtta acattaaaaa tggttaatag
      121 tttgcgcctc tcacacaggt ccaggcaact gcgtaacagg caaatcaaat tttcgtaaac
      181 agtgggcatc atcagatgcc cacaaaatac agctgattta cattttcgtc tttacacgtg
      241 caatagcttc ttcataagaa ggcttatttt tttccgtata cgccagttga taatgctgca
      301 atgcttcagc attctggccc gaggtttcat aaattttggc aatgttataa taggagttgg
      361 cgcgcgttgt cgccgcattt accccactcg ctaacgagat cgctttacga tttgcccaaa
      421 ttgcttccgc tatgttgcca tttttctgat acgccagacc caggttacta aatgcctgtc
      481 cgtaattgcc gtctaattct gttgcctgtt ggaagagttg aatggcttgc tcaagttgac
      541 cggttttata aacagcttca ccttcgcgat tatattttct tgccagttga gtatctccac
      601 tgttttgcat cgcaggcgaa gccggagctg aagagtccgt cgcaggcatt atcacagtgg
      661 ctaaatccct gacgtctaat gtggtgccgt taaaattggc gctggtaaca gagttgatat
      721 cttcaaactc gccgtttgga ttgagtttaa acacggtcca gatgttccct cgttttcctg
      781 cagggacact ataactacgt accagggagc tccctacata aacaaacact tttgccttac
      841 tggcagagag ataattactg ttgggcagcc ctttattgct atagtcctgc acggcataga
      901 tgtaactttc accaaaatgc tttttgctga tagtgacagt ttctggtcca tagctatccg
      961 tatcatcaac atccagattg gcatcagttc cctctttact atcaaaatag atatgtccgc
     1021 cagggaagat caaatgcgaa tcaagatcaa aaggcttttc gccccagctc aaaactacac
     1081 gcatgccatc cagattcgtc atcaccggac tgatagcata agtcatgcca gcgcacggac
     1141 atttgaccac cagattggaa tacccctctt ttttgacgat taatagagca tctttattat
     1201 cagcaaatga agtccccaaa ctaatgctgc ccgaagtatt tgtgatacca gaaacagatt
     1261 gtgcaccatt gcgttgcagc gtcaccgatg ccccttcaat gcgtttatct ttcactgtcg
     1321 cacttaaaat atcaatgctt acatcggcgg cataagcacc tgcattcatg cccaacagcg
     1381 cgagagcaac tggcaaaaca attttcttca tgtataaatc ctttttttaa tgaatgtaag
     1441 gtggaaattt cccagttatt attatcgtgt taaaaggtga aaaaaaagaa ataagtacac
     1501 ttttcagatt aacacccact caccttgaaa caaaaaggcc atccggagat gacctgaact
     1561 ttctgagaaa cgctgttttt tcatgtggtt tgctttatta cacaccattc agactattcg
     1621 gtagctaaat gtccgcgact gtcccggtaa aaagagagct aattctttta aaacctcatt
     1681 tttttctgta ttaaagcgtt cattcatcat ggtttctgta cacgtcatca ccttcctgct
     1741 aaacgcgacg gtcgcatcac agcttgtcaa ttcagccgga ttaaacagcc gcaatattaa
     1801 ttcctgtctg tcttctgctt tttttagcgc gctgatcaga catcccactg gtggcatttt
     1861 cagcaaacta tagctttccg gcacattgaa tttggcttta ttaagcttca tcgcatccca
     1921 cgggattttg ttataacact gaactggcgt cagccatgcg cgtgcctgct gggctacacc
     1981 ggcagttacg ggagtatcga tataactaaa taaactcaga cgacaggaaa atgatccgcg
     2041 taattgtgaa tcggacacag gcattttaat tcctgaagga cgccctggcc ttaaaagtaa
     2101 atcttctttt cccagtaatc ccacaccacg caacaacgta atagcaaagg ttttattctc
     2161 ttctccaatg acttcaaatt cacgtaatcc ttcgctaaaa acggctatgc cgttacgccc
     2221 ttcctgtaag acagcatagt tgagcatatt ccataccgga accggcgctt ctttccagcc
     2281 ttcttgctgc cagacattca ttgcgcaatc cttcactggc cgcgtcaatg aaccaaactg
     2341 cgtatccgca agaacaacat ctgtgttaaa aggcgtggga atcaatacac gaatacggtg
     2401 atcatcggcc tggttatcaa gattaatatc cgcatcaatg cgtctgctgt tatggctaag
     2461 agtgatcacc gtctccaccc ctaccctgcc agaacattgc tttgcgcgac gctctgacag
     2521 attacgcggc accgctattt catagcgaat tatggcccta ctctgccagg cttcatgaat
     2581 aatatcgtat tgcggtttcg cattcgctga ggtaatggcc cactcttctt ttgctggcga
     2641 ataatcatat tcatccccat catcggaacc ttcttcaatt tgaaacaccc ggtcatagcg
     2701 cacgccacta tctttatcaa ccagacgaag tgaaccatcc tcattgagtg caatttgcca
     2761 gaaagcattt tccagtattc cctctgtttt actttttggc gtgaccacat tgccaagctg
     2821 attcgcttct atatagagcg tgcgataccc catagaaggg acaatttggc tgatctgaat
     2881 atcaaactcc ataaagggat cataattgcc gtaatgaact atttgccgat caataaggcc
     2941 gggatcgatc tcacgggcat gtcgaataaa atatggcaca ggctgaccgc gaccgtctcg
     3001 caaattaaac tggctaccgc gcagtcgaac agttgtgttg ataacttctt cacgcggcca
     3061 gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc tgcggcatgt tgtctgctat
     3121 tttgcgcata tagaaccgaa ttagattatc cgccatgtct tctgccagtt cgaagcgggc
     3181 gacaatctcg cgatgaactt tatcactgca gcagcagccg atgctgtcat gggcgtggtt
     3241 ttttaaaatt tctttccaca tcttctccag caagccgtgg tgataatcaa aacctaaagt
     3301 ccaggccagc gttgccaacg gttccagcag attaacaatc ttattttcaa tgcgtgcgtg
     3361 ggcaattttg atatccatgc gcgtggaacc gatggtgcga tgcacgcgca tatatttgcc
     3421 atcgatgaat tcacctttca ggatggccag actttcacgc tgcgcttcaa tctgctcgaa
     3481 tacctcttca aagcggctca tcacaaactt tcgttgagga taaatttcgc gcagtttctc
     3541 catcacttcg aagatatttt gctgcaaggg catctggtca tgaccattcg gcaataaaat
     3601 ttctttggtt actgacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttacgtaa
     3661 tccattttcg tccgcaggta agtacttacc gatggcgtag cccagcggca gcacctgcgc
     3721 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatcag taccgtggcg
     3781 ctcagaacat ccgcgccaga acatggtgcg ggtaatgcca aatccattgt atatatgtgg
     3841 cagttgcccg gacatgccaa aggaatccgg taaataacct attttcatcg gctcgccaaa
     3901 cgcgaggcag tcacgcattc cgtacatcag attacggact atggattcag cagaaacaat
     3961 cgtggtatcg gtctgggtat accagggacc gataattaat ttcccgccct cgaccaactt
     4021 cttcacacga tctttgttct ccggtttgac agcgaaataa tcttcgagga tcgccgtttg
     4081 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtctgcaca ggatctcttc
     4141 catattattg accagcagaa tgcgagactc ttctgtggtg aaataccact cccgatccca
     4201 gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
     4261 tatggcatta cgccatcagt cagatagttt ccatgcttta ccgcatgatg ccgccagatc
     4321 aggagaattg cagtcgagat tgcagccccc accaacgccg caccaaacca accaattgcc
     4381 gccataacac cacccgcgcc attatcatga agtaaaaaga gcgagaaaat gccggcaccg
     4441 ggtgtcgaaa ggccgatatt catcgcaccg acgatagcgc ccgttaccat agatcccagc
     4501 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggtg
     4561 atacctgcca atcctaacag ccaggtggat ttcccggttt caatttcaaa ctctttaaac
     4621 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
     4681 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cccccagaca gaatgcataa
     4741 gcggctttgt tcactggccc accaaggtca aaggaacaca taaaaccgag aatggcgccc
     4801 aacaacagtg cgttatttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
     4861 catgcgacag gttcccctac tacaaacagc atcagactac ccgcccccaa cgtaccgagc
     4921 accgggtaga gatagaaagt caggaatccg ttgaatttac tgctaagacg tagatgattt
     4981 ttcacccagc gcatcagata accggctatc aatccgccaa cgaccgcgcc gagaaacccg
     5041 gaaccaatca tgttggcggc aagccctgcc gcaaagcctg gcgctaacgc cggtttatca
     5101 gccagtgaat aggcggtata ggctgcgagc accggtacca tcaaaatccc gagcatcccg
     5161 ccgcccagct tgcggtacat ccacaaccag gagttttctt cattaaacaa atcttgtagc
     5221 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tgccacccgc gacaatcagc
     5281 ggcacggcga aagagatccc gctcaacagt gcctgcttca gctccgtttt gacacttttc
     5341 accggttgcg tatcttgctg tacggtacgc gtctcatcgc tacgcttgag ggtaagcgct
     5401 tgttgaatca acgcttctgc atggcgaatc ggctcggcaa caggcacaga aagcgcggga
     5461 atgccgttaa aacgctcgct ctccttgata gcgacttccg ccgcaaaaat acaggcggtt
     5521 gcactattga gttgctccgc cgttaaacgc tcttcaatgc cgttagcacc ttgcttttca
     5581 acgtagatat tcacgccgag tttgcgtccg gctttttcca gatattccgc agccatgtag
     5641 gtgtgagcaa tacccgccgg gcaggccgtt acgcagacga tagttggcgc ataagaaaaa
     5701 gaggcagtat gttgcgcgtc ccccttgtca tccagcgccg agagcaattc atcgggcgtc
     5761 gttgccgact gaatacgcgc cctgagctca tcatccgcca ggcgcgtggt cagcgcggta
     5821 agcagtttca tatgcgtagt acctgcttca ttgggaggaa tggccagcaa taccactaaa
     5881 tcaaccgctt ccgggccatc cacgccttcc cactgaagcg gctcgctgag tgtggcgacc
     5941 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacggccag cccttcacct
     6001 aaggcggtcg ggccgaggct ttcacggcaa tacacttctt ccagaaattg ctcagtactg
     6061 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt ggatggcctc ttcacggctg
     6121 gtaaagcgca cattcagaca cagcgcatcg cggtgggtca gagtcgtcag gttcatcatg
     6181 gttctcactt gatcattttt atagtcgcgc aagtgtgcct gataaaacaa tacatttacg
     6241 tgatcacttt cacttacaag atactatttg tattaaattt gtattattta caagatgaat
     6301 ttatcgagca taatggctaa attcgtatta atgagatact agaaatggga cacaaaccct
     6361 tataccggca gattgccgat cgcattcgtg agcagattac gtgcggcgag ctaaaacctg
     6421 gcgatgcgtt acccactgaa tcggcattac aaacagagtt tggcgtcagc cgggttacgg
     6481 tgcgccaggc tttgcgccag ttagtcgagc agcagatcct cgaaagcatc cagggcagcg
     6541 ggacttacgt caaagaagag cgggtcaatt acgatatttt tcagttaacc agttttgatg
     6601 aaaaactgtc ggaccgtcac gtcgaaacac atagtgaagt cctgatattt gaagtaattc
     6661 cggctgacaa ttttcttcag cagcagctgc aaattagcga gcaggatcgt gtgtggcatg
     6721 tgaagcgcgt gcgctatcgc aagcaaaagc cggtggcgct ggaagaaacc tggatgccgc
     6781 ttgcattatt cccggatctc acctggcagg tcatggaaaa ttcgaaatat cactttattg
     6841 aagaagtgaa gaagatggtt atcgatcgta gcgaacagga gataattccc ctgatgccca
     6901 cggaagagat gagccgtctg cttaatatca gccagactaa gccgattctt gaaaaagtat
     6961 cgcgtggata tttagttgat ggccaggtgt ttgaatatag ccgcaacgct tttaatactg
     7021 atgactataa atttaccctg atagcgcagc gaaaatcgcc gcgataagca taaaaaaggc
     7081 catcataaga tggccaacca tgtcgaaacc gacagccctt cgcccttcct gtagggggaa
     7141 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
     7201 gatatcagcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
     7261 atccgcagta cctttaccac cggcaatgat ggcacccgcg tggcccatac gtttgccttt
     7321 cggcgcagtc acaccagcga tgtaacccac aactggcttg gtgacgtgct ctttgatgta
     7381 cgcagccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcagt
     7441 ctgcggatct ttttcgaaca tttcgagaat atcgataaag ttagagccag ggatcgggtc
     7501 gccgccgata ccaacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
     7561 ttcataggtc agcgtaccgg aacgggaaac gatacccact ttacctggtt tgtgaatgtg
     7621 acccggctgg ataccgattt tgcattcccc cggggtgata acgcctgggc agttcgggcc
     7681 gatcatacga acgcctgctt catccagttt cactttcacc gtcagcatat ccagcgtcgg
     7741 gatgccttca gtgatggtga taatcagttt gatgcctgca tcgatagctt ccagaatgga
     7801 gtctttgcag aacggtgctg gtacgtagat aacagaggcg gtagcgccag tggcagcaac
     7861 agcttcacgc acggtgttaa acaccggcag gccgaggtgg gtagtaccgc ctttacctgg
     7921 ggttacgccg ccaaccattt tagtgccgta tgcaatggcc tgttcagagt ggaaagtccc
     7981 ctggctacca gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
     8041 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
     8101 ataatattca ggccgctgtc agccagtttc ttcgcaccga gttcggcgtt gttaccttcc
     8161 agacgtacca cgaccggtac gttaacaccc acttctgcta ccgcgccgat gataccgtca
     8221 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
     8281 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccaccgcca
     8341 acgtccagga agttagccgg ttcgccgccg tgcagtttaa cgatgtccat cgtacccatc
     8401 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
     8461 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcacgc
     8521 agatcaggct ggcggaacag tgcgttgcca tcagcgccca gtttgccgtc gaggcaaatc
     8581 agatcgccct gtttggtaat aaccagcggg ttgatttcga taagcgcaag gtcgcgctcc
     8641 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
     8701 agacccagtt taaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
     8761 agcgcaactt tgtggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
     8821 ccttcggtgg aggccataaa gaccacgcga cgggaactac ggtcaacaac ggcaccgaga
     8881 tacagctctt tagcgatatc ggtcgctgct tcaaccagaa tctggttaac cggttggcca
     8941 ttggcatctg tttgatacgt taccagacgc ttgcccagcc agttttctgc aaaagcacgg
     9001 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
     9061 acctgacatt tcactaccca cggaccggca ccgatttttg aagcggcttc ttctgcttcg
     9121 cgtggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaaaagttgt
     9181 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
     9241 taaaccgtag accgaccccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
     9301 tacacgtcca gcagcagacg cgtcggatct tccagcaact ctttgatcgt taccaggaag
     9361 cccacggatt cgcgaccatc gatcaaacgg tgatcgtagg acagcgccag gtacatcatc
     9421 ggcaggatct caacctggcc attcaccgcc atcggacgat ctttgatagc gtgcataccc
     9481 agaattgcgc tctgcggcgg gttgatgata ggcgtagaca tcagggaacc gaacacacca
     9541 ccgttggtga tggtaaagtt accaccagtc agatcttcaa ccgtcagctt gccgtcacgg
     9601 cctttaactg ccagctcttt aattttcttc tcgatgtctg ccatgccgag ggtatcgaca
     9661 tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa ccgccatact gacgtcgaaa
     9721 tagttgtggt agaccacatc atcgccgtcg atagacgcat tcacttccgg gtaacgtttc
     9781 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
     9841 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
     9901 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
     9961 ttacgcaggc gagtcatcgg cacacgtttt tcgctacgtg cagccagggc cggttgcgcc
    10021 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    10081 acatcttcac gggtcagacg accacccaca cccgtgcctt taatggcgct ggcgtcgagg
    10141 ttatgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    10201 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    10261 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttacggtt
    10321 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    10381 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactac gtcgccgggt
    10441 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    10501 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    10561 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    10621 ggcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    10681 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    10741 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    10801 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ttgttccagc agatcgtaat
    10861 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    10921 cactgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    10981 gatgacgcag cagggatttc ggcgacatca cgaccagtgg acgacgcatc ccgcgcaacg
    11041 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga aggtacgcaa acctgcatgt
    11101 tttgctcagc gcaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    11161 gcccttcgta accgtgcggc agcaacatca ccaggccaca catccggccc catttctgtt
    11221 cgccagagga gatgaactgg tcgataacca cctgtgcacc gttggcgaag tcaccgaact
    11281 gtgcttccca gatggtcagg gtgcgtggtt ctgcggtggc ataaccgtat tcaaacgcca
    11341 gtacggcttc ttcagacagt acagagtccc agacgcggaa cgcgccctgg ccgttatgga
    11401 tatgttgcag cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    11461 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcgtcaa
    11521 ccaacgtggc gtaagcgagg ttttccgcgc cgccccagtc gaacagtttc tcaccggctg
    11581 ccatcgcctg gcgatcgcca taaattttgg caacgcgaga ctgcatttca accgcttccg
    11641 gcaccgtgct gatgcgttta gccagttcct gcaggcgctt catctcaact ttgttcgggt
    11701 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    11761 gacgccactc tgctacaacg caatcgccag catccagcgc atcgcggtac aggttaacca
    11821 tctcggtggc atcttccagc gtcgcgactt tttcctgctc cagcttgtca gcgtagattt
    11881 tgcgcggcgt cggatgtttt ttgattttct gatacatcag cggctgggtt gcgctcggct
    11941 cgtcggcttc gttgtggccg tgacggcggt agcataccag gtcgatgaag acatcacgtt
    12001 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    12061 ccgcattaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggcg
    12121 tcgaacgggc atccagcggg ttagaggtgg tgaagccaac ctggttgttg ataacgatac
    12181 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagggtt tcctgaacta
    12241 cgccctgccc ggtcactgcg gcgtcaccat gaatggtgat tggcagcact ttgttgctgc
    12301 tcggctcatc aagtctgtcc agacgggcac gaacagaacc gataacgacc gggcttacaa
    12361 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    12421 agtcagacga gaagcccatg tggtatttca cgtcgcccgt gccgaggtgt tctttatgtt
    12481 taccggcaaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    12541 tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg ggtgccgctg ttgccagcgt
    12601 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    12661 ttgcgccagg gaattttgcg ccaaggtaac gttcaaggcc ttcagcggcg gtcagttcgc
    12721 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    12781 ggatccagcg tttttcttcg gtgctggtaa tgtgcatata ctcggcacca atcgggccac
    12841 agtaggtttg cttgagggct tccagcagct cgccgagttt catggtttct ttgccgctgg
    12901 caaatgaacc gacgttgaag gtctcctgga agtctgcttc ggtcagatcg tggaaagacg
    12961 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatcgaga ttcgcatgct
    13021 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    13081 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    13141 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    13201 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    13261 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    13321 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    13381 accacgcaca tttcatatct gatgtgcgtg ccggggttca cttgttgcgg acttgtcgtg
    13441 ttttctgcgt ccgc
//
LOCUS       DAOFSV010000125        13388 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.125, whole genome shotgun sequence.
ACCESSION   DAOFSV010000125 DAOFSV010000000
VERSION     DAOFSV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.125"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(7..516)
                     /gene="crr"
                     /locus_tag="QBH27_003661"
     CDS             complement(7..516)
                     /gene="crr"
                     /locus_tag="QBH27_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDQ7015928.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(557..2284)
                     /gene="ptsI"
                     /locus_tag="QBH27_003662"
     CDS             complement(557..2284)
                     /gene="ptsI"
                     /locus_tag="QBH27_003662"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDQ7015929.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIIIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(2329..2586)
                     /gene="ptsH"
                     /locus_tag="QBH27_003663"
     CDS             complement(2329..2586)
                     /gene="ptsH"
                     /locus_tag="QBH27_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDQ7015930.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(2970..3941)
                     /gene="cysK"
                     /locus_tag="QBH27_003664"
     CDS             complement(2970..3941)
                     /gene="cysK"
                     /locus_tag="QBH27_003664"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDQ7015931.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(4126..4887)
                     /gene="cysZ"
                     /locus_tag="QBH27_003665"
     CDS             complement(4126..4887)
                     /gene="cysZ"
                     /locus_tag="QBH27_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDQ7015932.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            5117..6115
                     /gene="zipA"
                     /locus_tag="QBH27_003666"
     CDS             5117..6115
                     /gene="zipA"
                     /locus_tag="QBH27_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDQ7015933.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6186..8201
                     /gene="ligA"
                     /locus_tag="QBH27_003667"
     CDS             6186..8201
                     /gene="ligA"
                     /locus_tag="QBH27_003667"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDQ7015934.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            8203..8421
                     /locus_tag="QBH27_003668"
     CDS             8203..8421
                     /locus_tag="QBH27_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDQ7015935.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(8418..9416)
                     /locus_tag="QBH27_003669"
     CDS             complement(8418..9416)
                     /locus_tag="QBH27_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDQ7015936.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            9506..10432
                     /locus_tag="QBH27_003670"
     CDS             9506..10432
                     /locus_tag="QBH27_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7015937.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(10471..11235)
                     /locus_tag="QBH27_003671"
     CDS             complement(10471..11235)
                     /locus_tag="QBH27_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015938.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            11484..12317
                     /gene="xapA"
                     /locus_tag="QBH27_003672"
     CDS             11484..12317
                     /gene="xapA"
                     /locus_tag="QBH27_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDQ7015939.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGVPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            12378..>13388
                     /gene="xapB"
                     /locus_tag="QBH27_003673"
     CDS             12378..>13388
                     /gene="xapB"
                     /locus_tag="QBH27_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDQ7015940.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEV"
BASE COUNT         3336 a         3250 c         3444 g         3358 t
ORIGIN      
        1 caagaattac ttcttgatgc ggataaccgg ggtttcacct acggttacgc taccggacag
       61 tttgatcagt tctttgattt cgtccatgtt ggagataaca accggagtca gggtagactt
      121 ggctttctct tccagcagcg gcagatcaaa ttcaatgaca gtatcaccaa ctttcacgcg
      181 ctgaccttct tcagcaatac gcttgaagcc ttcgcctttc agttcaacgg tgtcgatacc
      241 gaagtggacg aacagctcaa cgccgctatc agattcgata gagaacgcgt ggttggtttc
      301 aaagatttta ccaatggtgc cgtctacagg cgcgaccatt ttgttacccg ttggtttgat
      361 agcaatgcca tcaccaacga ttttttccgc aaaaacgaca tccggcacgt cttcgatatt
      421 gacgatctcg ccagagagcg gagcaatgat ctcaatagtt ccggtatcct tcttgtcgtc
      481 ggaaaccaga gatttcagtt tatcgaacaa acccatgatc ttctcctaag cagtaaattg
      541 ggccgcatct cgtggattag cagattgttt tttcttcaat gaacttgtta accagcgtca
      601 ttaactcgtc cgttgtcggt tgagcaagag cctgctctgc taacaccttc gcatcttcga
      661 agttcgtgtt acggataatc ttcttaatgc gcgggataga aatggcgctc atagagaatt
      721 cgtccagacc catccccagc aacagaagtg tagcacgttc atcgccagca agctcaccac
      781 acatgccagt ccatttgcct tcagcatgag aagcatcaat aacttgcttg atcaagttca
      841 gcacggacgg tgacattggc tggtaaaggt gtgaaatcat atcattacca cggtcaactg
      901 ccagagtgta ctgcgttaaa tcattggtgc cgatactaaa gaaatcaact tctttggcta
      961 aatgacgtgc aattgttgcg gcagccggtg tttccaccat tacgccgatt tcaattgact
     1021 cgtcaaacgc tttaccttcg tcgcgcagtt cctgtttgta gatttcgatc tctttgcgca
     1081 gtgcacgcac ttcttcaaca gagatgatca tcgggaacat gatgcgcaat ttaccgaaag
     1141 cagaggcacg caggatagca cggagctgat cgcgcaggat ctctttacga tccatcgcga
     1201 tacggatagc gcgccagccg aggaacgggt tctcttcttt cgggaagttc atgtatggca
     1261 gctctttgtc gccgccgatg tccatggtac gaacgataac cgcctgcgag ccacacgctt
     1321 cagccactgc tttgtaagca gcaaactgtt cttcttcagt gggcaacgcg tcgcggtcca
     1381 tgaacaggaa ctcagtacga tacagaccaa cgccttcagc gccgttacgc tctgcacctt
     1441 caacgtcacg aaccgtacca atgttagcgc acacttctac ctggtgaccg tccagcgtaa
     1501 tagctggcag atctttcagt ttagcaagct ctgctttttc agaagccact tgctcctgaa
     1561 cagcgcgcat tttatcaata acttcgttgg ttggattgac gtaaacctga ttatttacgg
     1621 catccagaat cagatagtcg tcatttttca cctgagaggt gacgctaccg gtacccacga
     1681 tagcaggcag ttccagagaa cgcgccatga tagaggtgtg ggaagtacgg ccacccgcgt
     1741 cggtgatgaa acccagcacc ttcttcaggt tcagctgtgc ggtttcggac ggcgtcaggt
     1801 cagcggcaac cagaatgact tcatcctgaa tggcgctcag gtcgataatc ttcaggccca
     1861 ggatgttgcg cagcaggcgc ttaccgatat cacgtacgtc agccgcacgt tctttcaggt
     1921 attcatcatc cagctcttcc agggcagaag cctgaccttc gataacttca tgagcagctg
     1981 cgtcagctgt catgtgctta tctttaatca gggctatgat ttcctgctcc agctcctcat
     2041 cttcgagcag cataatatgc ccttcaaaga tggcttcttt ttcttcaccg aacgtttcac
     2101 cagctttcgt tttgatcgtt tccagctggg ctgatgcctt ggcacgaccg ctcagaaaac
     2161 gttcaacttc ctgatcaacc tggtcggcag aaattttttt ccggtcaatg ataatttcgt
     2221 cttctttcag aagcagagct ttaccgaaag cgatacccgg ggatgctaaa atgcctgaaa
     2281 tcataaccct accttacttg tgactgattt ttaaaagaac ccgggaaatt actcgagttc
     2341 cgccatcagt ttaaccagat gttcaaccgc tttctgctcg tcttcgcctt ctgcggagat
     2401 agtcacaacg gtaccttgag tcaggcccag agtctgcagt ttaaacaggc ttttcgcgct
     2461 ggcgcttttg ccgttggaag tcacagtaat ttcagaagtg aagcccttag cttcttttac
     2521 aaactgggca gcagggcggg tgtgcagacc gttcggagcg gtaatggtaa cttcttgctg
     2581 gaacattgta tttccccaac ttataggttt agtgttgtgg aactaaagtc tagcctggcg
     2641 gttcgacttt agcctgtatt gttagcgcca gtttttaaca gacgcgacgc acgaagagcg
     2701 taatacagtc cattgctggc gggtgttgac cgctgacgtt tcgtacgtca ttaaacatta
     2761 tgccgctaaa ggaagaattg aaccaaatca taaaatcgat tcagctttgg cttttcctgt
     2821 aacgattaat ttcgcgcatc aaaataatta gtctggttaa ataccagttc cgacaggctg
     2881 aatcaatgcc aggaggggcg aaagtttgaa gcaggccaca aaaaagcacc taaaaaggtg
     2941 cttttttacg cattttttac aagctggcat tactgttgca attctttctc agtgaagaga
     3001 tcggcaaaca atgcagtgct taaataacgc tcacccgatg acggcagaat aaccacaata
     3061 ttcttattgg taaagctttc atcttcttgt agtttcaacg ccgcagcaac agctgctcca
     3121 gaagagatac cagcaagaat gccttcttct tccatcagac gacgtgcggt agaaatggct
     3181 tcttcattgg tgatgccaat gactttgtcg accagcttga gatcaaggtt agccgggata
     3241 aaaccagcgc caataccctg aattttatgc ggaccaggtt taatctcttc acctgccagc
     3301 gcctgggcga taactggaga atcggttggc tcaacggcga cagagataag atcggtcttg
     3361 cctttggtgc ctttaatgta gcggctgacg ccagtcagcg taccgccagt cccaacgcca
     3421 gcaataaata catcaacctg accgtcggta tcttcccaga tctccggacc ggtggttttt
     3481 tcatgaattt cagggtttgc cggattgctg aattgttgca gcagcaggta tttctctgga
     3541 ttgctggcga caatttcttc tgctttttgg attgcgcctt tcatgccttt agcaccttcg
     3601 gtcagcacca ggtttgcacc taacgctttc agcagcttgc ggcgttcaat actcatggtt
     3661 tctggcatgg tcagggtaag tttgtaaccg cgagcggcag ctacataggc cagtgcaatc
     3721 ccggtattac cgctggttgg ttcaaccagt tcaacgcctg gtttcagcac gccgcgcttt
     3781 tcggcatccc aaatcatgtt ggcaccgata cggcacttaa cgctgaagct ggggttacga
     3841 gattccacct tcgccagaat gcgtccgtta ccgatgcgat tcaggcgaac cagcggcgtg
     3901 tgaccgatag tcagcgagtt atcttcaaaa atcttactca tggcctgtcc ttaactgtat
     3961 gaaattggga tacaacaggt agcatacccg ctcagagaat atgcggaagt aaggatttag
     4021 catatctata tgcagaaggg aaataatgac atgcaagatg gaataagggg cggcataagc
     4081 cacccctgtt tcacacaaac ggtttacaaa ataaccggta gattgttacc gccacatggc
     4141 gtgtttatcg cgatagcaat caacccacat cgccgtcgcg ccacaaacgg caacgggcat
     4201 aatgaacaga ttaagcagcg ggatcatcgt aaacaggctg gttaaagcac caaactgcat
     4261 attggtgatt ttgcgtgtgc gcagggcggt gcgcatctct ttaaacggca ctttgtggtt
     4321 atcgaagggg taatcacaat actggatggc taacatccag gcgctaaaca ggaaccacag
     4381 taccggcgcg acggtttgcc caatgccagg gatgaagtaa agaattagca atacaattgc
     4441 gcgcggcaga taccaggcaa atttttgcca ttcgcgtttc atgattcgcg gcacatcttt
     4501 catgataccg aaaattccgg tatctggcgg tgtagcgcca gtcagtcttg cttccagttg
     4561 ttcagccaat aaaccgttga acggagcagc aatccagtta gcaatcgttg agaagaaata
     4621 gccaaacact aacagcacag agatgacagc caggggccac aacagatagc tcagccattg
     4681 taaccagtcc ggaacataac tcatgagagt cgggatccag acatcgagtt gcgtaaagag
     4741 ccaccagaat gcgcccccca tcaacaaaat attgaccagc agcggtaaaa taacgaaacg
     4801 ccgaatccca ggttgcgaga cgagcttcca gccttgcgca aaatagtaaa aaccgctgcg
     4861 tggggcagat gtgaatgatg aaaccataat caggatgagc tccttttgac caatcccagg
     4921 aaaattctgc gtattttacc gggtaattgc gcaatggaca gttaggatat gttcgaaaaa
     4981 acagcaaaaa gcacgatttc atctatcttt gtgctgtgaa agttaatagt gcacttgcac
     5041 ttgaggtaat cggcaaatac tcttagtgag taaatgtttg ccgtggtggc aaggtgttag
     5101 aacaacagag aatataatga tgcaggattt gcgtctgata ttaatcattg ttggcgcgat
     5161 cgccataatc gctttactgg tacatggttt ctggaccagc cgtaaagaac gatcttctat
     5221 gttccgcgat cggccattaa aacgaatgaa gtcaaaacgt gacgacgatt cttatgacga
     5281 ggatgtcgaa gatgatgagg gcgttggtga ggttcgtgtt caccgcgtga atcatgcccc
     5341 ggctaacgcc caggagcatg aggttgctcg tccgtcgccg caacaccagt accaaccgcc
     5401 ttatgcgtct gcgcagccgc gtcaaccggt ccagcagccg cctgaagcgc aggtaccgcc
     5461 gcaacatgct ccgcgtccaa cgcagccggt gcagcaaccc gtgcagcagc ctgcctatca
     5521 gccgcagcct gaacagccgt tgcagcagcc agtttcgcca caggtcgcgt cagcgccgca
     5581 gccagtgcat tcagcaccgc aaccggcaca acaggctttc cagcctgcag aacccgtagc
     5641 ggcaccacag cctgagcctg ttgcggaacc ggctccagtt atggataaac cgaagcgcaa
     5701 agaagcggtg attatcatga acgtcgcggc gcatcacggt agcgagttaa acggtgaact
     5761 gcttcttaac agcattcaac aagcgggctt catttttggt gatatgaaca tttaccatcg
     5821 tcatcttagc ccggatggca gcggcccggc gttattcagc ctggcgaata tggtgaaacc
     5881 gggaaccttt gatcctgaaa tgaaggattt cactactccg ggtgtcacta tctttatgca
     5941 ggtaccgtct tacggtgacg agctgcagaa cttcaagctg atgctgcaat ctgcgcagca
     6001 tattgccgat gaagtgggtg gtgtagtgct tgacgatcag cgccgtatga tgactccgca
     6061 gaaattgcgc gagtaccagg acatcatccg cgaagtcaaa gacgccaacg cctgatacac
     6121 ttaaggcaaa ttaactcctt ttcgaacccc cgcttgtcgg gggtttttag cattgatggt
     6181 gcgatatgga atcaatcgaa caacaactga cagaactgcg aacgacgctt cgccatcatg
     6241 aatatcttta tcatgtgatg gatgcgccgg aaattcccga cgctgaatac gacaggctga
     6301 tgcgcgaact gcgcgagctg gaaaccaaac atccagaact gattacgcct gattcgccta
     6361 cccaacgtgt aggtgcagcg ccgctggcgg ctttcagcca gatccgccat gaagtgccaa
     6421 tgctgtcgct ggataacgtt tttgatgaag aaagctttct tgctttcaac aaacgtgtgc
     6481 aggaccgtct gaaaagcaac gagaaagtca cctggtgctg tgagctgaag ctggatggtc
     6541 ttgccgtcag tattctgtat gaaaatggcg ttttagtcag tgccgcgact cgtggcgatg
     6601 gcactaccgg ggaagatatc acgtctaatg tgcgtactat tcgcgccatt ccgctgaagc
     6661 tacacggtga gaatatcccg gcgcgtctgg aagtgcgtgg tgaagtgttc ctgccgcagg
     6721 cggggttcga aaagattaac gaagatgcgc gacgcacggg cgggaaagtg tttgctaacc
     6781 cacgtaatgc ggcagctggt tcactgcgtc agcttgatcc gcgtattaca gcgaagcgac
     6841 cgctcacttt cttctgctac ggcgttggtg ttctggaagg cggtgaattg ccggataccc
     6901 atttgggacg cttaatgcaa tttaaagcat ggggattacc tgtcagtgat cgggtaacac
     6961 tttgtgaatc ggcggaagaa gtgctggcgt tttatcacaa agtagaaaaa gaccgtccga
     7021 cgctgggctt tgatatcgac ggcgtggtga ttaaggtcaa ctcactggca cagcaggagc
     7081 agcttggctt tgtcgcgcgt gccccgcgct gggcggtagc gtttaaattc ccggcgcagg
     7141 aacagatgac ctttgtgcgt gacgtcgagt ttcaggttgg gcgtactggc gcgattacgc
     7201 ctgttgcgcg tctggaacct gtccatgttg ctggcgtgtt ggtgagtaac gctacactgc
     7261 ataatgctga cgaaatcgaa cgtcttggtt tacgcattgg cgataaagtg gttattcgcc
     7321 gcgctggcga cgtgatcccg caggtggtta acgtcgtgct ttctgaacgc ccggaagata
     7381 cccgtgaggt tgtattcccg acgcattgtc cggtatgtgg ttctgacgtc gagcgtgtgg
     7441 aaggtgaagc ggttgcccgc tgtaccggtg gcctgatttg cggtgcgcag cgtaaagagt
     7501 ctctgaaaca ctttgtttcc cgccgtgcga tggatgttga cggaatgggc gacaaaatca
     7561 tcgatcaact ggttgaaaaa gaatatgtcc acactccggc ggatctgttc aaactcaccg
     7621 caggcaaact gaccggactg gagcgtatgg ggccgaaatc ggcacaaaac gtggttaacg
     7681 cgctggaaaa agcgaaagaa accacctttg cccgcttcct ctatgcactt ggcatccgtg
     7741 aagtcggcga ggctaccgca gcaggtctgg cggcatattt cggcacgctg gaagcgctgg
     7801 aagccgcgtc gattgaagag ctgcaaaagg tgcctgatgt tggaattgtc gttgcctccc
     7861 acgttcacaa cttctttgcc gaagaaagca accgcaatgt catcagcgag ctgttggcgg
     7921 aaggtgttca ctggcctgag ccgatcgtta tcaatgcgga agagattgac agtccgtttg
     7981 ctggtaaaac cgtggtgctt acgggcagct taagccagat gtcgcgtgat gacgctaaag
     8041 ctcgactggt cgaactgggg gcgaaagtcg cgggcagcgt gtcgaagaaa accgatctgg
     8101 tgatagcggg tgaagctgca ggatctaaac tggcgaaggc gcaggaactg ggtattgaag
     8161 tcatcgacga aacggaaatg ctgcgtttgc tgggtagctg agatggaaaa agagcagctg
     8221 attgaaatag ccaatacgat aatgccgttt ggcaagtaca aagggcgtcg cttaatcgac
     8281 ctgccggagg agtatctgct gtggtttgcc cgcaaagatg agttcccggc agggaagctc
     8341 ggtgagctaa tgcaaatcac gctgctgatt aaaatcgagg ggctgacgca actggtccag
     8401 cccctgaaac gtccgcttta agctttatcg gcgctgcttt cctgctgcgc ctgtaactgt
     8461 tcggtctggc gtttgtatcg acgcgccagc accgcacaga ccatcaattg gatctgatgg
     8521 aaaatcatca ggggcagcac catcataccg atcaccgatg tggggaacag aatgtttgcc
     8581 atcgggatgc catttgccag actctttttc gaaccacaaa agacgatagt aatttcatct
     8641 gccttattga agcccagtcg gcgtgccata aagacgttaa ctacaatcac gatagccaga
     8701 agaacgcaac tgaccaccac gataaacagc aatgatcccc agccaacttt gtgccagata
     8761 ccgttaacga cggcttcgct gaatgccgta taaactacca acagaatgga cgtctggtca
     8821 gttttcgcaa tccatttttt attacgcgac acccagtcac caatccacgg ccgggaaaga
     8881 tgccccaaca caaatggcag caacagttgc agcataattt taccgacctg ctcaaggctg
     8941 ccccctgcac cgtgaacatt catcaccaga ccaaccagca atggtgaaag gaaaatcccc
     9001 agcaggctgg atgctgacgc agaacaaacc gccgccgcga cgttaccgcc cgccattgac
     9061 gtgaaggcga ttgcagactg cacggtcgcg gggagaatgc acaagtagag aaaaccggag
     9121 tagagcatcg ggtcgacatt taccggtttc caccaggcaa acagcacgcc cagaatcgga
     9181 aacagcacga aggtgctgca cattacccac aaatgcagtc gccagtggcc accgccagca
     9241 ataatcgcct cacgcgataa cttcgcgccg tgcataaaaa acagcagggc tatcgctgcg
     9301 gtggtcagat tttcaaagaa ggggacgaaa tcgcctctgg ccggaaagaa agaggccagc
     9361 aacaccaccg tgatcagggt taaggtgaaa ggatcgagga tacgaaaaag tttcataaag
     9421 actccagatg atcgatggtt ctattgtgcg tcttccagtt tgagaaataa aattgattta
     9481 ttgcatccat tcatgaaaaa aacagatgaa ttattcttta aagcaattaa aggttttcgt
     9541 cacagtagcg caggagaaaa gttttagtcg tgcaggagag cgtatcggcc tgagccagtc
     9601 ggcagtgagt cacagtgtga aggagctgga aaatcatact ggtgttcgcc tgctggacag
     9661 aaccacgcgt gaagtggtgc ttacagatgc agggcagcag ttggctttgc gtcttgagcg
     9721 actgctggat gaacttaaca gcacgttgcg cgataccggg cgtatggggc aacaactgag
     9781 cggaaaagtt cgggtcgctg ccagccagac catttccgcg catcttattc cgcaatgcat
     9841 tgctgaaagc catcgccgct atccagatat tcagtttgtc ctgcacgatc gtccgcagca
     9901 gtgggtgatg gaaagtattc gccagggaga tgtcgatttt ggcatcgtca tcgatcctgg
     9961 cccagtgggc gatctgcaat gtgaagcgat tctttccgag cctttctttc tgctttgcca
    10021 tcgcgatagc gctttggccg tggaagatta tgtcccctgg caggcattac agggagcgaa
    10081 attggtgttg caggattacg cgtcaggcag ccgaccgctg attgacgcag cgctggcgcg
    10141 caacggtatt caggcgaata ttgtgcagga gattggacat ccggcgacgc tgttcccgat
    10201 ggtagccgca ggcatcggca tcagtattct tcccgcactg gcgctaccat tgcctgaagg
    10261 tagcccactg gtggtaaagc gcattacgcc agtggttgaa cgccagttga tgctggtgcg
    10321 caggaagaac cgttcacttt ccaccgccgc tgaagcgtta tgggacgtag tacgagacca
    10381 gggcaatgcg ctaatggctg ggcgggaagg ggatccgttg tatcagatat agagtgaatt
    10441 catactgccg ggagctccgg cagtaatcaa tcagaaatta aatttgatgg agtagattat
    10501 cgcatcctgg taaaaatctt cgcccagttt gtcatccgca taacgatact gaatccctgt
    10561 ggtgatgtgt gaagttgcat tccaccaaag tgcgacggca ccgttcagac cttctttgcc
    10621 gccattacct gccgcgtagg tggcatcacg atcaaactcg tactcgttcc agttggtgag
    10681 ggtgaatttc tcactgccca gcataaagct gtaacctgca acccaaccgg cgacataacc
    10741 gttatcgccg gtataatagg tttggtctgt atagcgtttt gcaaagaacg gtttgaacca
    10801 ccaaccgctg ccggtgaaat tgtagccgat cccgtaaagg aacatatcgt catggaaatt
    10861 tacacgattc gcagaaccgt aggtgccata cgcatgcaag tagagattaa atccggtgtc
    10921 acccaggtaa atacggttgg tatttttaaa tgtataacgc tgctcgctac ccggtttatc
    10981 atgacgatca ttataaaagt tttcccagtc gaagaacccg tacatttctc cccaactaaa
    11041 gttagcgccg ccttccagtt ccagatatcc aaaatcatct ttgtgtgatt tggtcgacga
    11101 tttctcagtg gttctgctag tccagtcaag ataatgaata cctatatcag caaaaccgcc
    11161 tttaaattct gcatgggata ctgctggtat cgccaataca gaagagagtg tcagagtaaa
    11221 aagatgtttt ttcatagcat aattccctat gccgatcgtt attagttgat tgcttaataa
    11281 aaaggttgat taaaagcggc atattgttaa tgcaagggaa aggagagtac caatacagtt
    11341 ttttcagcct attaatagtg aaaaaatata aatatatcga tatcgaataa ctatcggata
    11401 catgaaaaaa gtgtattggt atgaggtagg gcaatctcgt tataaaaagg cgttagattc
    11461 ccccctacaa aaaaaggata tatatgtatc aggctcagtt ttctcataac ccactgtatt
    11521 gcgtagatat tatcaagact tataaacctg atttcacgcc acgagtggcc tttattttag
    11581 gttccgggct gggcgcgctg gccgatcaga ttgagaacgc ggtcgcaatt tcctacgaaa
    11641 agctgcctgg gttcccggta agtaccgtac acggtcatgc gggtgagctg gtgctggggt
    11701 atctccaggg ggtgccagtg gtgtgcatga aaggtcgcgg acatttctac gaaggtcgtg
    11761 ggatgaccat catgacggat gcaatccgta cctttaagtt gctgggctgc gagttgctgt
    11821 tctgcaccaa tgcggctggc tcactgcgcc ctgaagtggg ggccggcagt ctggtcgcat
    11881 tgaaagatca catcaacacc atgccgggaa cgccgatggt gggtcttaat gatgaacgtt
    11941 ttggtgagcg cttcttctcg ctggcgaatg cctacgatgc ggaataccgc gcactgttac
    12001 aaaaagtggc gaaagaagag ggggtccctc tgacggaggg cgtgttcgtc tcgtatccgg
    12061 ggccgaattt cgagactgcg gcggaaattc gcatgatgca aattattggt ggggatgttg
    12121 ttggtatgtc tgtggtgcct gaggttattt cagctcgcca ttgcgaactt aaagtcgttg
    12181 cggtctctgc gattaccaac atggcggaag gtctgagtga cgtgaagctt tctcatgccc
    12241 aaacgctggc agcagcggaa ctctcaaagc aaaactttat taatcttatt tgcggctttc
    12301 tgcgcaaaat tgcctgagta aaacaacaat aaacggcctt actgagtgag gccatatgat
    12361 gcgacaagga aacgattatg agcatcgctt tgcgcttaaa ggtaatgtcc tttttgcaat
    12421 attttatctg ggggagctgg ctggttaccc tcggttctta catgattaat acccttcatt
    12481 tcaccggcgc taatgttggc atggtttaca gttccaaagg gatcgccgcg attattatgc
    12541 ctggtataat ggggatcatc gcagacaaat ggctgcgtgc agaacgtgca tacatgctgt
    12601 gtcacctggt gtgtgcgggc gtactttttt atgcggcatc cgtaactgat ccggatatga
    12661 tgttttgggt gatgttagtc aatgcgatgg cgtttatgcc gactattgcg ttatcgaaca
    12721 gcgtctctta ttcctgtctt gcccaggcag ggcttgaccc ggtgaccgct ttcccgccca
    12781 ttcgcgtttt tggtacggtg gggttcattg tcgccatgtg ggcagtaagc ttgctgcatc
    12841 tggaattgag tagtctgcaa ctgtatatcg cgtccggtgc gtcattgctg ctgtcggctt
    12901 atgcgctgac tttgccgaag attccggttg cggagaaaaa agcgactaca tcgcttgcca
    12961 gcaagctggg tctggatgcc ttcgtgctgt ttaaaaatcc acgcatggct atctttttcc
    13021 tcttcgccat gatgctgggt gcggtgctgc aaattaccaa cgtttttggc aacccgttcc
    13081 tgcatgattt cgcccgtaat cctgagtttg ctgatagctt cgtggtgaaa tacccgtcca
    13141 ttttactgtc agtttcacag atggctgaag tcggtttcat tctgacgata ccgttctttt
    13201 tgaagcgttt cggtattaaa accgtcatgc tgatgagtat ggtggcgtgg acgctgcgct
    13261 ttggcttctt tgcctatggc gatccatcac caaccgggtt tattttgctg ttgctgtcga
    13321 tgattgttta cggttgtgca ttcgatttct tcaatatttc tggttcggtc tttgtcgaac
    13381 aggaagtt
//
LOCUS       DAOFSV010000126        13238 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.126, whole genome shotgun sequence.
ACCESSION   DAOFSV010000126 DAOFSV010000000
VERSION     DAOFSV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.126"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..281)
                     /locus_tag="QBH27_003674"
     CDS             complement(<1..281)
                     /locus_tag="QBH27_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDQ7015941.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSA"
     gene            complement(271..708)
                     /gene="ratA"
                     /locus_tag="QBH27_003675"
     CDS             complement(271..708)
                     /gene="ratA"
                     /locus_tag="QBH27_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDQ7015942.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            879..1361
                     /gene="smpB"
                     /locus_tag="QBH27_003676"
     CDS             879..1361
                     /gene="smpB"
                     /locus_tag="QBH27_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDQ7015943.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1576..1938
                     /gene="ssrA"
                     /locus_tag="QBH27_003677"
     tmRNA           1576..1938
                     /gene="ssrA"
                     /locus_tag="QBH27_003677"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2622..3599
                     /gene="csiD"
                     /locus_tag="QBH27_003678"
     CDS             2622..3599
                     /gene="csiD"
                     /locus_tag="QBH27_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDQ7015944.1"
                     /translation="MNALTAVQNNAVDSGQDYRGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFQVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNIQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGTLSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3619..4884
                     /gene="lhgO"
                     /locus_tag="QBH27_003679"
     CDS             3619..4884
                     /gene="lhgO"
                     /locus_tag="QBH27_003679"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDQ7015945.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDIYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKTQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFN"
     gene            4910..6358
                     /gene="gabD"
                     /locus_tag="QBH27_003680"
     CDS             4910..6358
                     /gene="gabD"
                     /locus_tag="QBH27_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDQ7015946.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAASRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDSVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6372..7652
                     /gene="gabT"
                     /locus_tag="QBH27_003681"
     CDS             6372..7652
                     /gene="gabT"
                     /locus_tag="QBH27_003681"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDQ7015947.1"
                     /translation="MISNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            7889..9289
                     /gene="gabP"
                     /locus_tag="QBH27_003682"
     CDS             7889..9289
                     /gene="gabP"
                     /locus_tag="QBH27_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDQ7015948.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKTIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTSAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            9310..9972
                     /gene="csiR"
                     /locus_tag="QBH27_003683"
     CDS             9310..9972
                     /gene="csiR"
                     /locus_tag="QBH27_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDQ7015949.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9973..10422)
                     /gene="kbp"
                     /locus_tag="QBH27_003684"
     CDS             complement(9973..10422)
                     /gene="kbp"
                     /locus_tag="QBH27_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDQ7015950.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10506..10664)
                     /locus_tag="QBH27_003685"
     CDS             complement(10506..10664)
                     /locus_tag="QBH27_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDQ7015951.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10847..11146
                     /locus_tag="QBH27_003686"
     CDS             10847..11146
                     /locus_tag="QBH27_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7015952.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDCQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11156..11680
                     /gene="ygaP"
                     /locus_tag="QBH27_003687"
     CDS             11156..11680
                     /gene="ygaP"
                     /locus_tag="QBH27_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDQ7015953.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLLAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11727..12131)
                     /gene="stpA"
                     /locus_tag="QBH27_003688"
     CDS             complement(11727..12131)
                     /gene="stpA"
                     /locus_tag="QBH27_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDQ7015954.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12799..>13238
                     /gene="alaE"
                     /locus_tag="QBH27_003689"
     CDS             12799..>13238
                     /gene="alaE"
                     /locus_tag="QBH27_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDQ7015955.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQV"
BASE COUNT         3239 a         3357 c         3408 g         3234 t
ORIGIN      
        1 gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag aggacgataa
       61 atctccaccc gatcgccatc atgcacaata tcgctgagtt ttgccggacg gctgtaaatg
      121 ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaaacc actggcgcga
      181 atagcttctt caaccgtcgc gccctcctgc aacgtcacgc gctgcaggta ctgcttctca
      241 ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtagacct cttttgcacg
      301 aacagtaaaa gcctgcacca tattagctgc cagctcttta aacacacgac caaaggcgag
      361 ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcaatacggc acgcgtcctg
      421 gctcaacggc gtaaacttcc aaccgcctat caatttctta aacggtccat caaccagact
      481 catgagaata ctttgattac tggtcaactg gttgcgggtt gtaaacgttt tgctgatccc
      541 agccttagac acatctaccg cagcggtcat ctgcccagga gtggactcca gaatccggct
      601 tccagtgcac cctggtaaaa actgaggata agactgaacg tcattcacta actgatacat
      661 ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa caatttccat
      721 caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc tatcccggcg
      781 ctgggtaaca tcgggatcat gctaagatag agccttgtcc cccgcaggat tgatatgggg
      841 tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa aaagcacata
      901 aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac tttatcgaag
      961 aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg cgtgcaggaa
     1021 aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt ctgtttggcg
     1081 ctaacatcac gccaatggct gtggcctcca cgcatgtggt gtgcgatcct acccgtaccc
     1141 gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgta aatcgagaag
     1201 gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg
     1261 gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa cgcgaatggc
     1321 aggtggataa agcgcgtatc atgaaaaacg cccatcgtta aacctatata agcgatacta
     1381 atcaaatcct caccgcgcct ccctatccgg tggcgcgaat gaacatcttg tcgactgtca
     1441 aatctgacac agatgtagcc atcccattgc ttaatcgaaa aaaaatcata ctatgcaggc
     1501 gctaattcca ggatgataac gccttttggg ctagtcatgc tgctcataaa tctggtatac
     1561 ttaccgttac acattggggc tgattctgga ttcgacggga tttgcgaaac ccaaggtgca
     1621 tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact
     1681 acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc gctcttagga
     1741 cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa
     1801 gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga
     1861 atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac gcgggttcaa
     1921 ctcccgccag ctccaccaaa taatgattgg acggtgtaag gactacacca acaaaaacag
     1981 gaagttataa gtctcagcag gacaccgacc agacggtgag gagacaaaaa aggatacgca
     2041 acggagtcgc gactccctat gacattaaag cccgctgatg cgggcttttt tattggtcct
     2101 gacccgccct gaatagaatt catccaatta cagtccagac atatacagta tccctcagct
     2161 cagtatcagc ttctcgacaa atggtcactg ttatttaccc gtttccagaa agagaataat
     2221 ttgcccccat agccagcctt cccggaatct gtatgttcat taatattgtt atcgatgcca
     2281 tcaagtaaca taggttggat gcgattgatc ttactcacat tagtcgacac ggaactcagt
     2341 aagttgaact ctgaaaaagc agtttaaata gcatttaaac aaatgccacc agttcgaaaa
     2401 agagtataag cacacttaat atattgtttt aattattcat ttcctttcaa cccaattctt
     2461 atccctcaca ttacttgtta ttaatttgct gtttttgatc acaataagaa aacaatatgt
     2521 aacttttatg cgcatttttc agaaatgtag atatttttag attatggcta cgaaatgagc
     2581 atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca ctgaccgccg
     2641 tacaaaataa cgctgtcgat tcaggccagg actatcgcgg attcactctc atcccgtcgg
     2701 cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa cagtttctcg
     2761 aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcagg
     2821 taggcaaaat tctcgacgat ctgtgtgcga atcagctgca accgctgctg ttgaagaccc
     2881 tgttaaaccg cgctgaaggt gcgctgttga tcaatgcggt gggtatcgat gatgtcgcgc
     2941 aggcggatga gatggtgaag ctggcgacgg cggtggcgca tctgattggc cgttccaatt
     3001 tcgatgccat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtt gataactcag
     3061 acagctatct tcgtcagccg caccgcgtaa tggagctgca caacgacggc acttacgtcg
     3121 aagagatcac tgattacgtg ctgatgatga aaatcgacga gcaaaacata cagggcggta
     3181 actcgctgct gctgcatctc gatgactggg aacatctgga cctctttttc cgccatccgc
     3241 tggcgcgccg tccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgtct
     3301 tccatccggt gttcgatgtc gatcagcagg gccgcccagt gatgcgctat atcgaccagt
     3361 ttgtccagcc gaaagacttc gaagaaggcg tgtggttgag cgagctttcg gacgccattg
     3421 aaaccagtaa aggcactctt tctgtacccg ttcccgttgg caaattcctg ttgattaaca
     3481 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac
     3541 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag
     3601 cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg
     3661 tcgaccgcca tgcaactgat tgatatctat cctgacgccc gcattgcgtt gctggaaaaa
     3721 gagtccggcc ctgcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggagtc
     3781 tattacacgc ccggtagcct gaagacgcag ttttgcctgg cggggaaccg cgccactaaa
     3841 gtcttttgcg atcaaaacgg cattcgctac gacaactgcg gcaagatgct ggtcgccacg
     3901 tctgaactcg aaatggaacg gatgaatgcg ttatggaaac gcacggcggc aaacgggatc
     3961 gagcgcgagt ggttaaacgc cgaggagctg cgcgagcgtg aaccgaatat caccgggctt
     4021 ggcggcattt ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac ggcggcgatg
     4081 gcaaaaatct tccaggccag aggtggtgag attatttata acgccgaagt cagcgccctc
     4141 agtgagcata aaaacggcgt ggtgatacgt acccgtcagg gcggtgaata tgaagcatcg
     4201 acgctgatta gttgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa
     4261 ccgggcttta ttatctgccc gttccgcggc gagtatttcc gccttgcgcc agagcataac
     4321 cagattgtta accacctgat ttaccccatt cccgacccgg caatgccgtt tttgggcgtg
     4381 catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttc
     4441 aaacgcgaag gctaccgcaa gcgcgacttc tcatttagtg acacgctgga gattttaggc
     4501 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa
     4561 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     4621 ttaagcgatc tccagccctg gccccccggt gtgcgggcgc aggcggtatc gccggacggc
     4681 aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac ctgtaatgcg
     4741 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     4801 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat
     4861 gccttacacg ccgcatttaa ttaataacct ttgaaaacag gatgtagcga tgaaacttaa
     4921 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     4981 tggcgaggtc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa
     5041 aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc agccgcgccc tgcccgcctg
     5101 gcgtgcgctc actgccaaag aacgcgccaa cattctgcgc aactggttca atttaatgat
     5161 ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac cactggctga
     5221 agcgaaaggc gaaatcagct acgccgcctc atttattgag tggtttgctg aagaaggcaa
     5281 acgtatttat ggcgacacca ttcccggtca tcaggccgat aaacgcctga ttgtgatcaa
     5341 gcagccgatt ggcgtcaccg cggctatcac gccatggaac ttcccggcgg cgatgattac
     5401 ccgtaaagct ggtccggcgc tggcggctgg ctgcaccatg gtgctgaagc ccgccagtca
     5461 gacgccgttc tctgcgctgg cgctggcaga actggcgatt cgcgcgggcg ttccggctgg
     5521 cgtgtttaac gtggtcaccg gttcggcggg cgcggtcggt aacgaactga ccagcaaccc
     5581 gctggtgcgc aagctgtcgt ttaccggctc gaccgaaatt ggccgtcagt taatggaaca
     5641 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcac cgtttattgt
     5701 ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgtaa
     5761 cgccgggcaa acctgcgtct gcgccaaccg cctgtatgtg caggacagcg tgtatgaccg
     5821 ctttgccgaa aaattgcagc aggcggtgag caaactgcac atcggcgacg ggctggagaa
     5881 aggcgtcacc atcgggccgt tgatcgatga aaaagcggta gcaaaagtgg aagagcatat
     5941 tgctgatgcg ctggagaaag gcgcgcgcgt ggtttgcggc ggtaaagcac atgaacgcgg
     6001 cggtaacttc ttccagccga ccattctggt agacgttccg gctaacgcca aagtgtcgaa
     6061 agaagagacg tttggccccc tcgccccgct gttccgtttt aaagatgaag ccgatgtgat
     6121 tgcgcaagcc aatgacaccg aatttggact tgcagcctat ttctacgccc gtgatttaag
     6181 ccgcgtcttc cgcgtgggcg aagcactgga gtacggcatc atcggcatca acaccggcat
     6241 tatttccaat gaagtggccc cgttcggcgg cattaaagcc tcgggtctgg gtcgtgaagg
     6301 ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact
     6361 ggagaatgcg aatgatcagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc
     6421 gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg
     6481 acgttgaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc
     6541 acctgcatcc gaaagtggtt gcggcggtgg aagcgcaatt gaaaaaactg tcgcacacct
     6601 gcttccaggt gctggcctac gagccgtatc tggagctgtg cgagattatg aatcagaagg
     6661 tgccgggcga ttttgccaag aaaactctgc tggttacgac cggttccgaa gcggtggaaa
     6721 atgcggtgaa aatcgcccgc gccgccacta aacgtagcgg tactatcgcc tttagcggtg
     6781 cgtatcacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg
     6841 cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg
     6901 gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat gacgccgcgc
     6961 cggaagatat cgccgccatc gtgattgagc ccgttcaggg cgaaggcggt ttctacgccg
     7021 cgacgccagc ctttatgcag cgtttacgcg cgctgtgtga cgagcacggg atcatgctga
     7081 ttgccgatga agtgcagagc ggcgcagggc gtaccggcac gctgtttgcg atggagcaaa
     7141 tgggcgtggc accagatctc accacctttg cgaaatcgat cgcaggcggt ttcccgctgg
     7201 cgggcgtcac cgggcgcgcg gaagtgatgg acgccgtcgc accgggcgga ctgggtggca
     7261 cctatgccgg taacccgatt gcctgcgtag cggcgctgga agtgttgaag gtgttcgagc
     7321 aggaaaatct gctgcaaaaa gccaacgttc tggggcagaa gctgaaagac ggattgctgg
     7381 cgatagccga aaaacacccg gagatcggcg acgtacgcgg tctgggggcg atgatcgcca
     7441 tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg
     7501 tggcacgcgc ccgcgataag ggtctgattc ttctctcctg cggcccgtat tacaacgtgc
     7561 tgcgcatcct tgtaccgctc accattgaag acgctcagat ccgtcagggt ctggagatca
     7621 tcagccagtg ttttgctgag gcgaagctgt agcaccgcac catgccggag gcgacgcttt
     7681 gcggcttgtc cggcctacga gaatccaggt cggataaggc gtttacgccg catccgacaa
     7741 tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg ctctccggca
     7801 aaaagaacca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg gcgctctccc
     7861 aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac atgagttagg
     7921 cggcgggctg aagtcacgcc acgtcaccat gttgtctatt gccggtgtta tcggcgcaag
     7981 tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat tactggccta
     8041 tctgttcgcc ggattactgg tggttatgat tatgcggatg ttggcggaaa tggcggttgc
     8101 cacgcccgat accggttcgt tttccaccta tgccgataaa accattggcc gctgggcggg
     8161 ctataccatc ggctggttgt actggtggtt ttgggtactg gttatcccac ttgaagccaa
     8221 catcgccgcc atgatcctgc actcatgggt tccaggcatt cccatctggt tattttccct
     8281 cgtcattacc ctcgccttaa ccggcagtaa tttattaagc gttaaaaact acggcgaatt
     8341 tgagttctgg ctggcgctgt gtaaagtcat cgctatcctg gcctttattt tccttggtgc
     8401 cgtcgcaatt agcggttttt acccgtatgc cgacgtgagc gggatttcaa gattgtggga
     8461 tagcggcggc tttatgccca acggcttcgg tgcggtatta agcgcgatgt tgatcaccat
     8521 gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg acacgccgga
     8581 aaaacatatt gttcgcgcca ccaactcggt tatctggcgt atctctattt tctatttgtg
     8641 ttctattttt gtcgttgttg cattaatccc gtggaatatg ccagggctga aagccgttgg
     8701 ttcttatcgc tcggttctgg aattgctcaa tattcctcat gcgaaattaa tcatggactg
     8761 cgtgatatta ctttccgtaa ccagttgcct gaactcggcg ctgtataccg cttccaggat
     8821 gctctactcc ttaagccgtc gcggtgatgc tcccgcggta atgggtaaaa tcaaccgcag
     8881 taaaaccccg tacgtagcgg tgttactctc caccagcgcg gcattcttaa cggtggtggt
     8941 gaactattac gcgcctgcga aggtgtttaa atttctgatc gacagctccg gcgctatcgc
     9001 cctgctggtt tatttagtca tcgccgtttc acaattgcgg atgcgcaaaa ttctgcgagc
     9061 agaaggaagc gaaattcgcc tgcggatgtg gctttatccg tggctcacct ggctggtcat
     9121 cggctttatt acctttgtgt tggtagtgat gctattccgc ccggcgcaac agttagaagt
     9181 gatctccacc ggcttattag cgattgggat tatctgtacc gtgccaatta tggctcgctg
     9241 gaaaaagctg ataatgtggc aaaaaacacc cattcataat acgcgctgat aatgatcagg
     9301 agtcacacca tgaccattac ctctctggat ggctatcgct ggctgaagaa cgatattatt
     9361 cgcggcaatt ttcagccgga tgaaaaatta cggatgagtt tgctgacatc gcgttatgca
     9421 cttggcgttg ggccgttgcg ggaagcactt tcgcaactgg tggcagaacg actggtcacg
     9481 gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaggagct gctcgatatt
     9541 ttcgacgccc gcgccaatat ggaagcgatg ctggtgagtc tggcgattgc ccgcggtggc
     9601 gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc tcagtaagct tgaggcctgt
     9661 gacgccagtg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt tcatactgca
     9721 attgtggcgg gctgtggttc ttactatttg ctgcaaatgc gtgaacggtt gtttgatctg
     9781 gcagcgcgtt atcgatttat ctggctgcgg cgaacagtgc tttcggtgga aatgctggaa
     9841 gataaacacg atcagcacca gaccctgact gcggcggtac tggcgcgaga taccgcgcgc
     9901 gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca gcaggcgatg
     9961 gctggcaatt aactactctt caggaatacg caacacttgc cccgggtaaa ttttatccgg
    10021 gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag cgttaccgta
    10081 gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa actggctggc
    10141 agtggctggt gtcgccgttt tcacctgatc atcgacactg gcaataccgg aaatattccc
    10201 caccgcaaca aggattttct ccttcgcctc ctgacttaag ccgtcaccag tgaccgtcgc
    10261 tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg ttttgttcag
    10321 atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca
    10381 gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct cattcgttaa
    10441 cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg ggggtgagca
    10501 caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat atagccaagc
    10561 agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag cagcacgccg
    10621 agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac tccctataag
    10681 aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt ttagcggagc
    10741 ctgccagttt cagcgtacgc ttttactctc atttacgctg ctgatgctgg tatatgttgc
    10801 acacttagtt tagagtttac ttaatttaga aaatacttaa acaactatga ctgaactcgc
    10861 acaattacag gccagtgccg aacaggcagc ggccttattg aaatcaatga gccaccctaa
    10921 acgattgctg attctgtgta tgcttagcgg ttcccccggc accagcgcgg gagagctgac
    10981 gcgcattacc ggactgagtg cctctgcgac atcacagcat ctcgctcgta tgcggggcga
    11041 agggcttatc gactgccaac gggatgccca acgcattcta tattccatta aaaatgaggc
    11101 ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc
    11161 tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat
    11221 cgatattcgt gatgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc
    11281 attatccgta ctggaacagt caggtcttcc gcctaaatta cgtcgcgagc aaattatatt
    11341 ccactgccag gcaggtaaac gcaccagtaa taacgctgat aaattagcag cgattgccgc
    11401 ccccgcagaa atctttttac tcgaagatgg gattgatggc tggaaaaaag caggattgcc
    11461 agtagcggta aataaatccc aacccttacc gttaatgcgc caggtgcaaa tcgccgcggg
    11521 tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcgttt tcttcttatt
    11581 aagcggcttt gttggtgctg ggttactgct tgcaggaatt agtggttttt gtggaatggc
    11641 aaggttgtta gataagatgc cgtggaacca acgagcttga gcagcgacgc cggacgcgcc
    11701 atagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct
    11761 gccagcgcct gagcgattgg cttcggtgta cggccctgac cggtccaggt tttagtttca
    11821 ccgttaacat cgatgaattt atatttcgcc ggacgcggct ggcgtttttt accagcgcgt
    11881 ggtgccgcag cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc
    11941 agctccagcc aggtgctaat tttttcctgg cgctctgcca gttcacgctg ctgttgttct
    12001 tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg
    12061 tcaatggaga attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac
    12121 attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta
    12181 gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt cctgcacgag
    12241 attatttcag ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca
    12301 ttttgatgag taatttcggt tattggtgaa cagagcggct ctctctgctc gctaattccg
    12361 caagcctgac aaaaccaaag tgaataaaaa tgcaattcaa ccgaataaaa gaaaacatct
    12421 atcactggtg ctttctgctg tcaatgtaca ttatctgtca taaaaaatgc tgaaaatgac
    12481 ccggtcacta catacaccga attctctacg cttatagcgt tacctcaccc ccaaaccttc
    12541 atgagtgaaa ataaaggcaa gcacatatcc aattgattta attaaaaata aatcatatcg
    12601 gcatgtaaaa atccacaatg tacaaaaaac gcgccgttac ggaatatttt atctacaaaa
    12661 acagattaaa tgaaaatttt tcactaattg attagtcata gccagcgata tacgctatgc
    12721 aaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt
    12781 ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat
    12841 acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc
    12901 ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta
    12961 attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc
    13021 ccgtcaggct ggataaaaaa tctggcggat atcctggctt atgtgacgtt ccagtcaccg
    13081 gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcagtc
    13141 agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat
    13201 tgccgccgac tgtttaaagt cagccgttac cagcaggt
//
LOCUS       DAOFSV010000127        13124 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.127, whole genome shotgun sequence.
ACCESSION   DAOFSV010000127 DAOFSV010000000
VERSION     DAOFSV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.127"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            7..405
                     /gene="mutT"
                     /locus_tag="QBH27_003690"
     CDS             7..405
                     /gene="mutT"
                     /locus_tag="QBH27_003690"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDQ7015956.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFSGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVE"
     gene            complement(493..690)
                     /gene="yacG"
                     /locus_tag="QBH27_003691"
     CDS             complement(493..690)
                     /gene="yacG"
                     /locus_tag="QBH27_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDQ7015957.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(700..1443)
                     /gene="zapD"
                     /locus_tag="QBH27_003692"
     CDS             complement(700..1443)
                     /gene="zapD"
                     /locus_tag="QBH27_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDQ7015958.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            complement(1443..2063)
                     /gene="coaE"
                     /locus_tag="QBH27_003693"
     CDS             complement(1443..2063)
                     /gene="coaE"
                     /locus_tag="QBH27_003693"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDQ7015959.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            2088..2132
                     /locus_tag="QBH27_003694"
     CDS             2088..2132
                     /locus_tag="QBH27_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015960.1"
                     /translation="MIIKTLKMSARKRN"
     gene            2288..3331
                     /gene="guaC"
                     /locus_tag="QBH27_003695"
     CDS             2288..3331
                     /gene="guaC"
                     /locus_tag="QBH27_003695"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDQ7015961.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQRW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(3366..4568)
                     /gene="hofC"
                     /locus_tag="QBH27_003696"
     CDS             complement(3366..4568)
                     /gene="hofC"
                     /locus_tag="QBH27_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDQ7015962.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(4558..5943)
                     /gene="gspE"
                     /locus_tag="QBH27_003697"
                     /pseudo
     CDS             complement(4558..5943)
                     /gene="gspE"
                     /locus_tag="QBH27_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            complement(5953..6393)
                     /gene="ppdD"
                     /locus_tag="QBH27_003698"
     CDS             complement(5953..6393)
                     /gene="ppdD"
                     /locus_tag="QBH27_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDQ7015963.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            complement(6597..7490)
                     /gene="nadC"
                     /locus_tag="QBH27_003699"
     CDS             complement(6597..7490)
                     /gene="nadC"
                     /locus_tag="QBH27_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDQ7015964.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            7578..8129
                     /gene="ampD"
                     /locus_tag="QBH27_003700"
     CDS             7578..8129
                     /gene="ampD"
                     /locus_tag="QBH27_003700"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDQ7015965.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            8126..8980
                     /gene="ampE"
                     /locus_tag="QBH27_003701"
     CDS             8126..8980
                     /gene="ampE"
                     /locus_tag="QBH27_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDQ7015966.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHAHATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHALDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(9023..10393)
                     /gene="aroP"
                     /locus_tag="QBH27_003702"
     CDS             complement(9023..10393)
                     /gene="aroP"
                     /locus_tag="QBH27_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDQ7015967.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            10921..12702
                     /gene="hcp"
                     /locus_tag="QBH27_003703"
     CDS             10921..12702
                     /gene="hcp"
                     /locus_tag="QBH27_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDQ7015968.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGNGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVTVDYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKVKGCTDAGTAEEPAENFGQVA
                     IFVQPVVDDCCGYHHPEANVIGEPAEAQTMLLFPSSVLATQIWGKWSLSGVLSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIVDKLRGREFKTFDEF
                     REALWLEVSQEPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKKFQIHHFIAI
                     EYGGGVYDIDNLRIVTPRLHDEIHYRR"
     gene            12705..12989
                     /locus_tag="QBH27_003704"
     CDS             12705..12989
                     /locus_tag="QBH27_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDQ7015969.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSSMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSK"
BASE COUNT         3244 a         3195 c         3374 g         3311 t
ORIGIN      
        1 aagacaatga aaaagctgca aattgcggta ggtattattc gcaacgagaa caatgaaatc
       61 tttataacgc gtcgcgcagc agatgcgcac atggcgaata aactggagtt ttccggcggt
      121 aaaattgaaa tgggcgaaac gccggaacag gcagtggtac gtgaacttca ggaagaagtc
      181 gggattaccc cacaacattt ttcgctattt gaaaaactgg aatatgaatt cccggacagg
      241 catataactc tgtggttctg gctggtcgaa agctgggaag gggagccgtg gggtaaagaa
      301 gggcaacccg gtgagtggat gtcgctggtc ggtcttaatg ccgatgattt tccgccagcc
      361 aatgaaccgg taattgcgaa acttaagcgt gtgtatgtcg aataaggcgt cacgctgcat
      421 ccgacatttg catatgatgc ctgatgcgac gctggcgcgt tttatcaggc cagcatcgca
      481 tcagatagaa tgtcactgct ttggctcttc gctccagtca tcgctttcgg aaagatcgcc
      541 actgctgggg attcgttttt cttcagcagc ccattctccg aggtcgatca gctgacaacg
      601 tttggagcaa aatggccgaa acgggctgat ttcaccccac accaccgttt tcccgcaggt
      661 tgggcaattc accgtaatag tttctgacat ttttactcct tagcaacagg ccagttcgaa
      721 atccagacgt tctggtactt gtccgttttc actgtccagc ggcataaaac gaatggcaaa
      781 acggctctta tgaccggaaa tttgcggata aagctgtgaa tcgagcgaca gattcaggcg
      841 cagcaagtcg gcatcgccac cgttatcctg ataaaaacca ttcaggctgg tttgtttacg
      901 gaagggggcc gactggcgaa ttaaatccag caccatggta agtgcctggg taagtgggtt
      961 caggctggca atccaggttt ctacctggct gtcgcgctgc gcctggggta gatgcagcca
     1021 aatgtgcaat gtaggtaaat caaagctgca acagccgccc gggatgctca gtcgctgacg
     1081 caccagagca atcaaacgat cttcacgtag aaattgcccg atacgcggcg cggaaattaa
     1141 tacgctcccc gccgctttta actgctgaat taatgcttca atacggctct ggtccacgcc
     1201 gggcacgcca atccaggtct ggagtttacg ttgctgccgg tcaagttctt tcaacagctc
     1261 agtgcggact tcgccgcgct cgaaaacatc cagtaattcg ctgacattac ggaagaaatg
     1321 cagcgcgcca gcgtggtcaa cgatgggtaa attaacggtg agttgctgaa tcaaaaactc
     1381 aatgcgcagc catgtacgca ttttttcatt tagtggatgt tcaaaaagga cctgggtctg
     1441 cattacggtt tttcctgtga gacaaactgc gacgcaagct gcaaatagtg tgcgtgcagg
     1501 cgggcaacat ccgatgcgat agcatccggt gcgccgttat tatcaatgac gtcatctgcc
     1561 acggcaaggc gagcttcgcg cgttgcctga gcagcaagga tttgttcgac atgctcgcga
     1621 gttacatcgt cgcgctgcat ggtgcgctta agttgcgttt ctgggctgac atccaccacc
     1681 agcactcgat tcgctttttt atacagtgag ttttctacca gcaatggcac aacccatagt
     1741 acataggggg aagtagcttg ctggatctgg tgttgcgtct cttgctgaat cagcggatgc
     1801 agcagggcgt taagccagtt tttctcttcc gggttggcga agatccgctc gcgcaaggcc
     1861 cggcgctgca atgttccatc agcagcaatc atgttagcgc caaagtgatc agcaatggca
     1921 tgtagcgcag gtgcacctgg ttcaaccacc tgacgcgcaa taatatcggc atcaatgacg
     1981 ttaattccga gatcagcaaa cgcattggca acggtactct tgccactgcc aatgcctccc
     2041 gttaaggcaa ctatatacct cataatttat attcccggga attcatcatg attatcaaaa
     2101 cgttaaaaat gagcgcacga aagcgaaatt gatgaaacgt tcgctcacta tttaccaggt
     2161 aaatttatgg gattgtagcg taaaaaaaga caatttcgca gtcttgcgcc gcattgatta
     2221 gtgcgtatga taacgtcact ggagttgcgc tcttaccctt atagccatta accccaggaa
     2281 tccgcacatg cgtattgaag aagatctgaa gttaggtttt aaagacgttc tcatccgccc
     2341 taaacgctcc actcttaaaa gccgttccga tgttgaactg gaacgtcaat tcaccttcaa
     2401 acattcaggt cagcgctggt ccggcgtgcc aattatcgcc gccaatatgg acaccgtagg
     2461 cacattttct atggcctctg cgctggcttc ttttgatatt ttgactgctg tgcataaaca
     2521 ctattctgtc gaagagtggc aagcgtttat caacaattct tccgctgatg tgctgaaaca
     2581 tgtgatggtt tctaccggca cgtctgatgc ggatttcgaa aaaactaaac agattctcga
     2641 cctgaacccg gcattaaact tcgtttgtat tgacgtggcg aatggttatt ccgaacactt
     2701 cgtgcagttc gttgcgaaag cgcgtgaagc gtggccgacc aaaaccattt gtgctggcaa
     2761 cgtagtgact ggtgaaatgt gtgaggagct tatcctctcc ggtgctgata tcgttaaagt
     2821 tggcattggc ccaggttctg tttgtacaac tcgcgtcaaa acaggcgtcg gttatccgca
     2881 actttctgcg gtgatcgaat gtgccgatgc tgcgcatggt ctgggcggaa tgatcgtcag
     2941 cgacggtggc tgcaccacgc cgggcgatgt cgcgaaagcc tttggcggcg gtgccgattt
     3001 cgtcatgctt ggcggcatgc tggcgggcca cgaagagagc ggcggtcgca tcgttgagga
     3061 gaacggcgag aaatttatgc tgttctacgg catgagctca gagtctgcga tgaaacgtca
     3121 cgttggcggt gttgcggaat atcgcgcagc agaaggtaaa accgttaagc tgccgctgcg
     3181 aggcccggtt gaaaataccg cgcgtgatat tttgggcggc ctgcgttcag cttgtacata
     3241 cgttggggct tcacgcctga aagaactgac caagcgcacc acatttatcc gtgtgcagga
     3301 acaagaaaac cgcatcttca acaacctgta atataccaac gctggcgtgg agcaacacgc
     3361 cagcgttatc ccattccact catcgcatcg cctaaatgga aaattggcag atacattgcc
     3421 accaccagcg taccgataat tcctcccgtt atgatcagca acgccggttc cagtaaggct
     3481 gcgaggttat ccgccagcgc cattgtgtta tcccgatgat gatgggcgag gttgtctaac
     3541 atgagatcca gcgagccgga tgcctctcct gttctcacta attgcaaaca gagcgggcta
     3601 aactcaccgg cattttttag cgccagccag atggggtgac cgttactgat atcgtgctgg
     3661 atttgtgtca gaagttgcac ccagtacgga cagcgcattg tttctctgac gctctcaacg
     3721 ccctgtaaaa aagaaatgcc tgcactttgt gtcagcgtca gaatcgtaaa gatctgcgtg
     3781 agtttttgtc cccgcatcag tgaacccata atcgggatgc gtaacagtaa tttctgccgt
     3841 gcgataagcc tggttgggtg gcgcatcagc agcttattgg ctatcgccag cagaaagccg
     3901 aatagcacca gcaaccaacc ccattcgcca ctaaaacttg ccagcgtcat gatcccctgc
     3961 gttagcgccg gtagcggagt gttaaaggtc ttatagatag cggcaaactc cggcagaaca
     4021 aaatgcagca ttgccacaac caccatgatt gccatcgcta aaatgataat gggataacgt
     4081 aacgctgatt tcactttgtc ggtcaactga cgctgggctt tttgctgacg cgccagttca
     4141 aagcagcatt catccagctt accggtcagt tcacctgtgc ggatcatcgc ctgatagagc
     4201 ggcggaaata cctctgacca gggtaataag gcattggaaa aggcaacgcc ctgttcaaga
     4261 tcgtgcgcca gtgattgcag caacgcttgc cactgcttac tgggatgctg ttccgccagc
     4321 agagccagcc cttcagaaag cgttaacccg gctttgagta gcgtcgccag ttgatgaatg
     4381 acttccgcgc ttttatctcc tcgccactgc gcagaattga tggcgattcg cttcaggctt
     4441 agtggggtaa ccatctgttg ctgtagtgcc atgagcagca aagcacggct ctctgcccat
     4501 agcatcccat cttgcgcatt gccgtcgccg gttatgccac gccagcgcca gagttgctta
     4561 ctcgccatgc ggcatcccca atacgcggat taactcttca aaggtggtta agccttgctc
     4621 cacagccagg cagccgtttt caaaaagcgt tcgcataccc gcctgtcggg cgtgcgtttc
     4681 cagcgattca acgtcggtat tagcggaaat aagctgacgt atgaccggtg ttatgggcag
     4741 aacttcaaat aaggccgtgc gaccataaaa accgtggtag caatgtacac aaccgggtgc
     4801 ctgtcagtgg ggcagcggcg acggccatac attgtctgga atatggatgg gctccccttg
     4861 ctgccggcga caatgcgggc aaagtttgcg taccagacgc tgggctatta ccagcgtaag
     4921 cgccgatgat agcatccagc gggcgactcc catttgctgt aaacgtacca gcgtttcgca
     4981 ggtggaatta gtgtgtaggg tagacaacac caggtgaccg gtttgcgcgg cttttatggc
     5041 aatttctgcc gtttcgccat cgcggatctc tccgatcatg atgacgtcag gatcctggcg
     5101 caataacgca cgcaaaacgc cctgaaaggt gagtccggca cgcgaatgga tttgcgtctg
     5161 gtttaatccg gctatgggga tctcaaccgg atcttcgacg ctacaaatat taatgtcagc
     5221 ggtattcagc gtttgcaggg cactataaag cgtgaccgtt ttgccgctgc ctgtagggcc
     5281 agttaccagc accagtccct gtggttgttg caaggcatga gcaaagtcca ccagttgtga
     5341 cggctgcatt ccaagcgtgt taacatccag tgcctggttc acctgctgta acaaccttaa
     5401 taccaccttt tcaccacccc gacatgctaa ggtcgcaata cgaaatgaga cggcgtttcc
     5461 tgccagttcg acagtgaatt gcccgtcctg cggcaggcga tgttccgcaa tatccaggtt
     5521 tcccagcact tttaatctgg cggttaatgc gactccggca tccggtgaaa cgtccggtaa
     5581 aggatgcaat acgccgtcga tacgcaagcg gatgcggtag gcattgtccg ctggttcgat
     5641 atgaatatca gacgcgcgtt gttctagcgc agattgtaac gttcgagtca gcaactctgc
     5701 tttgggctga tgcttctcct gaacagctac gggcaatgtc tgttgtgtgc gactggcgtg
     5761 accttccatt tgttggcgcg tccagcaggt gatctcaata cgtttggtgg tagcgaaatg
     5821 caatgcgtcc agtagctcat gcgaaggtgc atcgactacc gcaacatgaa ccacctcttc
     5881 gctggcatcc agcaagactc cctgataacg cagacacagg gcagtgagct gtggaatatt
     5941 cattgccgct ccttagttgg cgtcatcaaa gcggaagaca tcttcgcagg cttgctgcaa
     6001 tgcgctgtca ctttgaatat tgcagttacg cgtccagccg gtgacgccgt ttgcgttatc
     6061 ccaacccggt gtcatgacga cgcttagccc attgagactt tcttgcccgg tcagcgacac
     6121 cacgcccttt gccacactca tggctgaaac atagcgggtg gtggtaggcg aaggaatgcc
     6181 attgctgcca ccgtcgcagg tatttaatcc accatgttcc agcgcgcaca actctacggc
     6241 ggtacggtaa ggcacaaagg tttgtagcat gtcggtgagt gcggctttgc gcaggtagtt
     6301 ttgataagcg ggaataccaa tggcgcttaa aatggcaatg atgccaataa ccaccatcag
     6361 ttcaataagt gtaaaaccgc gttgcttgtc catctgtttc gctccttgat ttggttggcg
     6421 ctactttggc aaacgccatc agcacaagcg aggggcaaaa aacgaaacgg gaaagcagct
     6481 tccgaggttt tttacttcgt tgcagcgaaa gacaagaaat ttgcgaggcg ttacgaagaa
     6541 agttggggaa ggggagattt atccgcccgc gatggagcgg ataaatctgt tagcaattag
     6601 cgaaaacgca ttgaaaggtc gagtgcctgt acgtgtttag ttagtgcacc aacggagata
     6661 aagtccacgc ccgtttcggc aaattcgcgc agtgttttgt cagtgacgtt gccagacact
     6721 tccagtagcg ccttgccgtt ggtgcgtttg acggcttcgc gcatctgttc tgtttcgaag
     6781 ttatccagca tgatgatatc ggcacctgct ttcagggctt catcaagttc ttccagattc
     6841 tctacttcga cttctactgg cgcatccggg tgcaaccagg acgctttttc gaccgcctgc
     6901 cgcactgagc cggaggcaat aatatggttt tctttgatca ggaaggcatc agaaagcccc
     6961 agacggtgat tcgctccgcc gccgcaaagt accgcgtatt tcagagctga acgcaggccg
     7021 ggtaaggttt tgcgcgtatc caacagctgc gtgttggtgc cttccagtaa ttcgacatag
     7081 tggcgtacct tactggcaac tcctgaaagg gtttgcacaa aattaagcgc agtgcgttcg
     7141 cccgttaaca gcacgcgaga tgggccttca agttcgaaca agggttgatt ggcgttgatg
     7201 acatcgccgt catccacatg ccagattatg gtgacatcgt cgcctgccag ttgaataaac
     7261 acctcttcaa cccagcgttt gccgcaaaat acgccattct cgcgggtgat cactgtggca
     7321 tgagagcgag agttttccgg taaaagtttt gccgtaatgt cattgttggc atcgactgtt
     7381 ccgcctaaat cttcccgcag cgcctgggcc accgcgccgg ggatatcgag attaatgcgt
     7441 tccagcagct cgtcacgtcg ggtgtcaggg ttatagcggc gaggcggcat gttaaaactc
     7501 cagatagcta acgaatcata aggtagaaac atgctactct gaaccgggta ttagcaccac
     7561 atataaggag atcctgcatg ttgttagaac aggggtggct ggctggcgcg cgccgcgttc
     7621 cctcaccaca ttacgattgc cgcccggatg acgaaacacc caccctgctg gtggtgcaca
     7681 atattagcct gccgccaggc gagtttggcg gtccgtggat cgacgcatta ttcactggaa
     7741 ctattgatcc gcaggcgcat cctttctttg cggagatcgc ccatttgcgc gtctccgctc
     7801 actgtttgat tcgccgtgat ggtgaaatag tccagtatgt tcctttcgat aaacgagcct
     7861 ggcatgcggg agtctctcag tatcaggggc gcgaacgctg caatgatttt tctattggga
     7921 ttgagctgga aggcaccgat acgctggcgt ataccgatgc gcagtatcaa cagcttgcgg
     7981 cggttacacg ggcactgatt gatcgctatc cggatatcgc taataacatg acgggccatt
     8041 gtgatattgc gccggatcgg aaaaccgatc ccggtcctgc atttgattgg gcacggtttc
     8101 gtgcgctggt cagcaaggag acaacatgac gctatttaca accttactgg tgttaatttt
     8161 cgagcgcctg tttaagttgg gcgagcactg gcagcttgat catcgtcttg aagcgttctt
     8221 tcggcgggtg aaacattttt ctctcgggcg cacgttatgc atgaccatta ttgcgatggg
     8281 cgtgactttt ttactgttac gcgcattgca gggtgtattg ttcaacgttc ccacgttgct
     8341 ggtgtggctg ctgattggtt tgctgtgtat tggcgcaggt aaagttcgtc ttcattatca
     8401 tgcttatctg acagctgctt cacgtaatga tagccatgcc catgccacga tggctggcga
     8461 actcaccatg attcacggcg tcccggcagg ctgcgatgaa cgtgagtatt tgcgtgagct
     8521 gcaaaatgca ttgctgtgga ttaactttcg tttttatctt gctccgctgt tctggctgat
     8581 tgtgggggga acctggggac ccgttacgct gatggggtat gcgtttttgc gtgcatggca
     8641 atactggctg gcgcgatacc agacgccgca tcatcgttta cagtccggca ttgatgccgt
     8701 gcttcatgca ctggattggg tgccggttcg tcttgcgggt gtggtatatg ccttgatcgg
     8761 tcatggtgag aaagcgttac cggcctggtt tgcttcgctg ggtgatttcc atacttcgca
     8821 atatcaggta ttaacgcgtc tggcacagtt ctctctggcg cgtgaaccgc atgtcgataa
     8881 ggtggagacg ccaaaggccg cggtttcaat ggcgaagaaa acctcgttcg tggtcgtggt
     8941 ggtaattgcg ctactgacga tttacggggc gttggtgtaa agattattgc cctcaccctg
     9001 cacgggtgag ggcgtagaga gattaatgcg cttttacggc tttggcggtt ttctctttaa
     9061 acagatagcc gatacctaac acgatcagcc ataccgggat caggtatacc gaaatcgcca
     9121 ttcctggggt catcagcata atcaccagta ccgccgccat aaacagcagg cagatccagt
     9181 tacccagcgg ataaagcagg gcagggaagc gagttaccac gccttgttct tgtttggcac
     9241 gacggaattt catatgcgcc agactgatca tcgcccagtt gatgaccagt gcagatacca
     9301 ccagcgccat taacagtccg aaagcggact ctggggcaag gtagttaatc agtacgcaca
     9361 atgccgtaac cagcgcagac accagaatag tgttaaccgg tacgccgcgt ttatcgacag
     9421 acgccagcgc ttttggcgcg ttcccttgtt gtgccagacc gaacagcata cggctgttgc
     9481 aatatacgca gctgttgtac acggagagcg cagcagtcag taccacgata ttcagcgcat
     9541 tcgctacaaa ggtatcgcct aactcgtgga agatcagcac aaacggactg gtatcggcgg
     9601 taacgcgggt ccacggcatt agtgagagca gaacggctaa cgagccaata tagaaaatca
     9661 ggatgcggta gataacctgg ttagttgctt tcgggatact ttgctccggg ttatcagctt
     9721 ctgctgcggt gatccccacc agttccagac caccgaaaga gaacatgata atcgccatca
     9781 tcatcaccag accggtgaag ccgtgtggca ggaaaccacc ctgatcccac aggttgctaa
     9841 cgctcgcctg cgggccgccg ttgccgctga atagcagcca ggcgccgaag atgatcatcg
     9901 ctaccaccgc gataactttg ataatggcaa accagaactc catctcgcca aacactttta
     9961 cgtttgtcag gttgatggcg ttgatcacca caaagaacac ggcggcagaa acccaggttg
    10021 ggatttccgg ataccagaac tgaatgtatt tacccacggc agtcagctct gccatggcaa
    10081 ctaaaacgta cagtacccag tagttccagc cagaggcgaa accggcaaaa ctgccccagt
    10141 atttataagc aaagtggcta aaggagcctg cgacaggttc ttcgaccacc atttcaccta
    10201 gctgacgcat gatcagaaag gcgataaaac cagcaatggc gtaacccagg ataatccctg
    10261 gccctgcgga ctgtattacg gaggcgctac ccaggaatag ccctgtccct atcgcgccac
    10321 ccagcgcgat aagctgaata tggcggtttt taaggccgcg ctttagctgc tcgccgtgct
    10381 gttgaccttc cattatgaaa cctcgtgttg tgttgttttt ttgaactacg cagcaatgcg
    10441 tgtgtaattt tgtaattccg tttgttgtat tggtttcttt acattttatg taactgaatt
    10501 atgatgatag tactttcaac tatcacaaga taatggtata cgcgcgagaa tgcacctgct
    10561 ttatgtagat gtgttgattt tctggataaa aaaaaagcaa ttgtaagttt ttcatcacat
    10621 ttaatagata ctttaagtct gataacatac tgttttaact agctatatac cggatttgtc
    10681 tgcctgctga acaccatgcc ttagccacca gcacaataat cttacaaaag gtcatataag
    10741 aataaataga tgttaataat acaatgggtt tgtaacgcga gtatttaaat ataatattgc
    10801 gagctaagtg aaaacgttaa gtaattgaaa taatttttca ttaaaagcgt ttcttattga
    10861 tcgagaaata agttttctat ttaatgcaaa taagttaagg ataaacggga atgaaataaa
    10921 gtgggcgata ttgtttacct gagaataatc ggtgagaagc agggcgatat ttcttcaggc
    10981 tgtgggacgt atgcgtcggt tggtaatcgc tggcaggttg gccatgaaga tgaaattttt
    11041 gcctttgcac tcaccaacgc cattaccagt actggtaatg gcgttaatct acaggggcta
    11101 caattttgca aactcattga taaaagctca ccgctactgt ctaatgccat caatcagaat
    11161 gagcggttat ttattgaaat cgatttgtat cgtataaata aaagcgggcg atgggaacgg
    11221 tattattata ttcagctaag aaatgcttca ttaactgcta ttcatgtaaa catttctgac
    11281 aataatcttc ctaccgaatg tgtaactgtc gattatgact acatattatg taaacatctc
    11341 atagccaata cggaatttga ctggttggcc tttcctgctg gctataatag cttatttatt
    11401 ccgcctaaaa gtccacctgc cagtaatctt aagcctgaac cgctaccagt tgttaacctt
    11461 ccactctctt caccagcggt taacccggtc tatgccaaat cctgtctgaa ggtgaaggga
    11521 tgtacagatg ccggaacggc agaagaaccc gctgaaaact tcggtcaagt agcgattttc
    11581 gttcagccag tggttgatga ctgctgtgga taccaccatc ccgaggctaa cgttatcggg
    11641 gaacccgcag aagctcaaac catgctactg ttcccgagta gtgtgttggc gactcaaata
    11701 tggggaaaat ggtcgctcag tggcgtactc agtgcaaccc gcggctctta catcggtgcg
    11761 ttggcatctg ctctgtatat tccctctgcg ggtgagggca gtgctcgtgt gcctggacgt
    11821 gatgagttct ggtatgagga agaactgcgg cagaaagcac tagcaggcag taccgccacc
    11881 acccgggtgc gttttttctg gggaactgac attcacggca agcctcaggt gtatggtgtt
    11941 catacgggtg aaggtacgcc gtatgaaaac gtccgcgtgg cgaacatgca gtggaacgag
    12001 cagacgcagc gttatgaatt tacccccgct cacgatgtcg atggccccct gattacctgg
    12061 acgccggaaa atccggaaca tgggaatgtt ccgggccata ccggtaacga caggccgccg
    12121 ctggagcagc ccaccattct ggtgacgccg attccggacg gcaccgatac ctataccacg
    12181 ccgccattcc cggttcctga tccgaaagaa ttcaacgatt atattctggt ttttccggcg
    12241 ggatccggta ttaagcctat ctatgtttac ctgaaggagg atccgcgaaa gctgcctggt
    12301 gttgtaacag ggcacggcgt cccgctttca ccaggaactc gctggctgga tatgtcggta
    12361 tccaataacg gcaacggcgc accaatcccg gcgcatattg ttgataaatt gcgcggacgg
    12421 gagtttaaaa cctttgatga gtttcgcgag gcgctgtggc tggaggtgag tcaggaaccg
    12481 gagttgatag cgcagttttc ggaaattaat cagttacgta ttagtcaggg ctttacacct
    12541 tttgcccccg acgaaggaca ctatattggt cctaaagaaa cacttaaaaa atttcagata
    12601 catcatttta ttgctattga atatggcggc ggcgtatacg atattgataa tttacgaatt
    12661 gttaccccca gactacacga tgagattcac tacaggagat aaatatgttt aaagaaaagc
    12721 tcgaagatta taccgaggag gaatttctta attttttagg tggattgaga tcatctatga
    12781 aagatggaaa gccattaaaa ggtaaggagc tagaaatgta ctgggattca ttagtagatc
    12841 attttattga aattacacag catccgtcag ggagcgatct aattttctac cctaaaagtc
    12901 aaggagatga taagcctgaa aacatcctga aaattgtcaa agaatggcgg cgttcacagg
    12961 ggttaccctt gtttaaagac tctaaataaa gtagataata aaactatata tggaacatca
    13021 tcgcatctgg ttaatgcgat gatattaatg acataaaata ataatgtgat tgctcttaaa
    13081 aaacaggtaa aaatactgaa gcctcattag gttattttat catt
//
LOCUS       DAOFSV010000128        12971 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.128, whole genome shotgun sequence.
ACCESSION   DAOFSV010000128 DAOFSV010000000
VERSION     DAOFSV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.128"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(56..541)
                     /locus_tag="QBH27_003705"
     CDS             complement(56..541)
                     /locus_tag="QBH27_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDQ7015970.1"
                     /translation="MKTVSQNTCTLSASVVTASENDQPQLVATLVPHEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDHLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALRHPECNAIMRII
                     D"
     gene            complement(632..1450)
                     /locus_tag="QBH27_003706"
     CDS             complement(632..1450)
                     /locus_tag="QBH27_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDQ7015971.1"
                     /translation="MQLASRFGHVNQIRRDRPLTREELMQVVPSVFGEDKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPLARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMTETLLENMR"
     gene            complement(1540..1716)
                     /locus_tag="QBH27_003707"
     CDS             complement(1540..1716)
                     /locus_tag="QBH27_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDQ7015972.1"
                     /translation="MAVTTAYRNVLIEDDQGTHFRLVIHNAEGQLRWRCWNFEPDAGK
                     QLNPYLASEGILRQ"
     gene            complement(1741..2418)
                     /locus_tag="QBH27_003708"
     CDS             complement(1741..2418)
                     /locus_tag="QBH27_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015973.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEVGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTPAINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            complement(2566..3246)
                     /locus_tag="QBH27_003709"
     CDS             complement(2566..3246)
                     /locus_tag="QBH27_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDQ7015974.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMTGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMAERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(3449..4333)
                     /locus_tag="QBH27_003710"
     CDS             complement(3449..4333)
                     /locus_tag="QBH27_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDQ7015975.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQALSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDDAVARFSFPAWMLQLLR
                     KTRDRIIHLLITLWERLF"
     gene            4439..5389
                     /locus_tag="QBH27_003711"
     CDS             4439..5389
                     /locus_tag="QBH27_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015976.1"
                     /translation="MASLSINTLRRMSICKSQAWKDTQRILKRSGLVYRGERSESFDP
                     EKHFNRYTVRYLYLLNIIALKIRSVSKFSCWIEVGQCHRMTGKHLSLNAPPFMLIPRS
                     IRRKVDGFRQSKGEATPKSAQPFTGSLYEVLSKESDSAKLDAWIAELPLNLQEEGKEI
                     SVMGFSPRAVARPVLRSASPTFELFYQEYQSLRLKSVFKFEPRFEQLLTRLSFRKQSI
                     LRKQQYRVKSQLLERLGNVLFFTSLYNQGCLEEFINALVEKEDIYLKMSPGEEKVKAH
                     QKMVNYIEAFCNKMTEKYLMPAASRHYKKKKIARSESGES"
     gene            complement(5386..6174)
                     /locus_tag="QBH27_003712"
     CDS             complement(5386..6174)
                     /locus_tag="QBH27_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015977.1"
                     /translation="MKQYKGGYNVNRYDWKNKTTEHLLNDNNLADNHIPEWIRKNEIA
                     AFWFWLIMSHTVPEKLKLNNESEFNSALKGVIVMKDNPMTIIPSSHETRLNVIGKWIN
                     ALILYGEADRIISIMHQNWLGLEKRNEAKWLKKNTEQLKWAWEYIDARISPTYLSWFN
                     PVNDNERYIAIVVLLKMLFPTDTPCLLTPKEFHLGFAAKERFFDKMHNAFRKQFIDGK
                     KDKRVQINVKISPSAKSALDRLTRERKTTQQAILEQLILYGRLD"
     gene            complement(7513..7761)
                     /locus_tag="QBH27_003713"
     CDS             complement(7513..7761)
                     /locus_tag="QBH27_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020236933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015978.1"
                     /translation="MIETEQTIPERKDNPAHSSKTRNNSMNGFKNDMDDEKSLKGKGS
                     TEHSNKTPETQPERFLAMQINNNTKTYSLTTQLNHRAE"
     gene            7866..8606
                     /locus_tag="QBH27_003714"
     CDS             7866..8606
                     /locus_tag="QBH27_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDQ7015979.1"
                     /translation="MNQPIHNAYWLSRFDILLDSALAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATSLRYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSIGDFSEPSSLATMIQEAWCSALHLEPWQGDGLVHFSRWTPSRKPA
                     SSDARPSSDDTPLSGGCSDTWKASDKKPGEAAVLWIKRGDVGALQKARNRASYLVKYE
                     TKLHNGSGQRNYGCSRGPGRLLDGRRSL"
     gene            9380..11428
                     /gene="ireA"
                     /locus_tag="QBH27_003715"
     CDS             9380..11428
                     /gene="ireA"
                     /locus_tag="QBH27_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HDQ7015980.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLIKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSIKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYSNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWHVSANYTFTDSRRKSDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
     gene            complement(11608..12820)
                     /locus_tag="QBH27_003716"
                     /pseudo
     CDS             complement(11608..12820)
                     /locus_tag="QBH27_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            12822..>12971
                     /locus_tag="QBH27_003717"
     CDS             12822..>12971
                     /locus_tag="QBH27_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7015981.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQDEYDSQRAAICSIAPKTGCTP
                     ETLRVW"
BASE COUNT         3387 a         2820 c         3167 g         3597 t
ORIGIN      
        1 ccgggcgtca tctctcctgg cagaaaggaa agctgttgca ttgttccagc ccctttcagt
       61 caatgatgcg cataatggcg ttgcattccg gatgccgcag ggcgtagtcc cgcagacggt
      121 aataatgaac cgtcatggca taacactccg tgcgacaggc atgatgactg tacgtcatca
      181 gacaggcagc aatgccggcg gcttccgggc tcatttcagc acggttaccg ttcatggcat
      241 tgaacagtac ccatgtctcg tcatcatcgt catccggttc gggtgccata aatgccccgc
      301 cgttgttcag ggtgtacaga ttccagatac caccgcagta gtcttcgcaa agatggtcca
      361 tccagccgaa gacacggggt tccagggtca cccactgtgg aatgaggcca aaatgctgcg
      421 gccagaagct gatgcgctgt tcatgaggaa cgagagtggc aaccagttga ggctggtcat
      481 tctctgaagc tgtgactacg gaagcagaaa gtgtacaggt gttctgagaa acagttttca
      541 tgagtgaaat ccttataaaa caaataaatg cagggttttc ggggagcagg gggtagggga
      601 gcctgaatca cgtcttcagg gatgtgctgc atcagcgcat gttctccagc agcgtttctg
      661 tcattaccca caatgcgcga ttgagcttaa tgtccgtgtc gatgctgtga atggcacggg
      721 tgtggatacg ttttccttta gcgctgcgac cggaaatccc gcctttcagc atattctcct
      781 ggatggtctg atatgcgctc cacaggtcct taccgtaatc ctcccggcgt cgcggcgtca
      841 gaatgtcggc ggtggtgacg ggctgatgtt cgtcaccata acggtaagtc agtgccgcct
      901 gtgccagcgc ctggcgtgcc agtggcggca ggaccagcga ctgcatggcg tcacgctttt
      961 cttcaatccg gtcaaaaacc cccaccacct cgtaagcccc ttcgataact ttctccacta
     1021 catttccccg gtgtggaaca cgcacttccc ccagagactg accacagacg catccgttct
     1081 ggcagacgaa cctgaagtaa cccggcagca tctggtagct ggaggtaccg tcatgggagt
     1141 tcagcaggat gatttcaggg acatgttgtc cgtttatctc cccgtcacgg cgcagacgca
     1201 gcatgtgttt ggtgtatccc cggcggcccg ggtcgcgcac acgggtctgg caggcgaaga
     1261 atggctgaaa accttcccgc tgcaggcttt ccagcactgt gatggtgggg atccaggtat
     1321 agttctcgct tctggatgtg tgcttatctt ccccgaatac gctggggacg acctgcatca
     1381 gttcttcacg agttaaggga cgatcacggc gtatctgatt tacatgacca aaacgactgg
     1441 ctaattgcat actttgctcc ttatgaatga gttagcggta taaatgaaaa ggccgcgtct
     1501 cccggagaag acgcggcctg atggatgaaa tgaagacgtt tattgcctga gaattccctc
     1561 actggcgaga tatggattta gctgttttcc ggcatcaggt tcaaaattcc agcaccgcca
     1621 gcgtagctgc ccttcagcat tgtggataac cagccggaaa tgcgttccct gatcatcttc
     1681 aataagcaca ttgcggtaag ctgttgtgac agccatcgcc gtgacagtcc gacataccgg
     1741 ctattttccg ataaattcaa cgctggcacc ggtggcatta ccgtcagcac caaccgtgac
     1801 ggtcactttg tcgtacgcac ggtcttcagg attgaacact tcaaactcaa ccttattgcc
     1861 ttttacttcg taagagccaa aatcagcggt ttctgttgtt tcctcgtcat attcccgggc
     1921 gtatgcctgc cgggcagcct gcaggacgga atctttatta atggcgggtg tctgtttcgg
     1981 ggaaaaacca gcaagtgatg tgacaaattt cggacagacc ttcagcatat caccgcgggt
     2041 gaattccatt cgtacaccaa atgcctgtaa aacggacaca tcacgctctg ctatggagtg
     2101 aacggctttt ttgaccattt ccggcgcctg tccgacttcc acctcaacag ccctgaatcc
     2161 cgtctgctga ttacacattt gtgatgtggg gatcaggtta ccgttaatac tgatacggct
     2221 gcccaggagc gtctgtacac cattcgggca tccctgcatc aggccggtaa taccaatcgt
     2281 gccatttttc agcattacca gcgcctgacc atcttccgtc atggtctgac accagccgtt
     2341 tttgacagcc cagttgccgg cggaggcgga gaatgatgcc acagtgaaca gcaggggaaa
     2401 aagagctctg gtattcatga ctgacctctg aaatgaacgt acggtgttat tgataatgat
     2461 ggaaggtatg cagtgactgt tcagggaatg acgtagccgt atatttagtt ttctgttcaa
     2521 catattgtct ccatacctgt tgggacggct ttggcaggcc ggcgatcaca ggttaaaggt
     2581 gttgataagt ttatggatga aagagaaatg tggcagggcg gagataaaac gctcttcagc
     2641 gaccagactg tggatgtggc ctctccgttc aaaccgttct gatgtcaggc tgactgattt
     2701 gatatctgcc agaggaaaga cctgcagttt tcccttctgt aacgcgatca gataccagct
     2761 accgctgtaa taaatgagcc ggcagggctc cagtgacgga taccatcgct cagccatcag
     2821 agagatgtgg atacactgtc ttattgccag gatgagttgc gagaaacact cggggaacgt
     2881 tgcagtgata tccggcgccg gtagccagat aaggcaggga gagggctcct ggttgtcggt
     2941 aagacaggtt atcagtcgtc cgttccggag cggaagtatc cgtgcgatcc cggtattctg
     3001 tataaaagaa agcatttcag ggatcgcacc cgggctgctc tgtcgtctga ggctgtacct
     3061 gccatttctg tactctaaat ccaggtgaac cagacgctga tgaaaatcgc gctgtaaagt
     3121 acgttctgta acgccaaatt catctgacag tgtttttagt gacagagatt ctccggtcat
     3181 cagtcggctg ataatgagtg ataagcgaac cgccaggcga tcatgccggg aacgattatc
     3241 agacatagat gtgcactccg taagaaaaca tcttttattt catgatgtta ttatgcatca
     3301 tgatgttgaa aggatgtgtc gcatccggat aatcaggaat aaaatgcagc agaaaaaata
     3361 ttcttttaat tatctgattt ttaagcatga tttcataaaa agaagaatgc tcgttaatcc
     3421 agcgacacat cctgtcggcg tgagtgtgtc agaacagacg ctcccacagt gtgatcagca
     3481 ggtggataat gcggtcccgg gttttgcgca gaagctgtaa catccaggcc ggaaaactga
     3541 aacgggcaac ggcgtcgtca aaacttttcc cgatggtttc accaaaagtc tgttgtgcgt
     3601 gtttccgggc ctgttcagag cggttttctc cccggatatg tgaatacacc gcgctggttg
     3661 cctgtggtgg cagtgcgtgg atcatcagcg acacaaacgc cggaaggttc cagccgacag
     3721 gtgcagcaac ggagagtact ggaaatgatg aagggaacag ggttgccacc cgggctgtca
     3781 ccgtcgccag tgagagcgcc tgctgacggg acgggcattt ttctgtggca ttccattctt
     3841 cagcaggaaa cacgcgatcg gcatggctga gaacaaacag aaaacgcgag ggatctgcgc
     3901 cttcattcag taaaaactga tgcatggtaa tgtcggcggc atacgcgcgt tcatctgccc
     3961 gcaggatcca gataatcaga tccagttccg gtagcagctg acgataaagt gtccggtatt
     4021 cctgatcatg ctgcggtgtt tcaccaatgc cgggcagatc gaccagcgtc attctgcgtt
     4081 caccgagttg cagggtaaga cgatgagcct ggcgggtgca gccgttcagg ggatgcgtgg
     4141 cgcagatacg ggactgaaaa atggcattac acaggctgct tttgccggta ccggatttac
     4201 ccataatgcc aatcacgggc tcgtaatgaa taacactgtg gagatgttca aggattttct
     4261 ggcgaatttc ttcaggaaga aacgtcagat atgatttcac ttgtgcggag agggaagaat
     4321 ggctgaatga cataccggta tcctgttgat ttcactgaac cggatcacac taatccggtt
     4381 tatcattcag gtaatatggg cgtgaaaaaa tctggagtga caaagttcgg gaggagtgat
     4441 ggcatcactg tcgattaata cgctacgccg aatgagcatt tgtaaaagtc aggcctggaa
     4501 agatacccag cgaatactga agcgtagtgg acttgtgtac agaggggaac ggtcagaatc
     4561 gtttgatcct gagaaacatt ttaatcgtta tacggtgcgt tatctgtacc tgcttaacat
     4621 aattgcgctt aagataaggt cggttagtaa attttcctgt tggatcgagg tgggacaatg
     4681 ccatcgtatg actggtaaac atttatctct taatgccccg ccttttatgc tgattccgcg
     4741 tagtatccgg agaaaagtgg atggattcag acagtcaaaa ggtgaagcaa ccccaaaatc
     4801 ggctcaaccg ttcacgggca gtttgtatga ggttttgagc aaggagagcg attcagctaa
     4861 gctggacgca tggattgctg aactgcctct gaacttgcag gaggagggaa aggaaatctc
     4921 agtcatggga tttagtccac gggctgtggc ccgacctgtt cttcggtcag catccccgac
     4981 gtttgaactg ttttatcagg agtatcaatc cctaagatta aaatctgttt ttaaatttga
     5041 accaagattt gaacaacttt taacaagact gagctttaga aaacaatcaa ttctcagaaa
     5101 acaacaatac agggttaaat cgcaattact ggagagactg gggaatgtgt tgtttttcac
     5161 gtcgctttat aaccagggat gtttagagga gtttataaat gcacttgtgg aaaaggagga
     5221 catttatctg aaaatgtccc ctggggagga aaaggtgaaa gcacaccaga aaatggttaa
     5281 ctacattgaa gcgttctgca acaaaatgac ggaaaaatac ctgatgcccg cagcaagcag
     5341 acactacaaa aagaagaaaa tcgctaggag cgaaagtggt gaaagttaat ccagtctacc
     5401 gtacagaatc agttgttcca gaatagcctg ctgtgttgtt ttccgctctc tggtcagtct
     5461 gtccagagcg ctttttgcgg atggcgatat ctttacgttg atttgtaccc gtttatcttt
     5521 ttttccatcg ataaactgtt ttctgaatgc attgtgcatc ttgtcgaaaa atctttcttt
     5581 tgcagcgaat cctaggtgaa attcttttgg cgtcagtaaa cagggtgtgt ctgtggggaa
     5641 taacattttt agtaaaacta caatggcaat atatctttcg ttatcattta caggattaaa
     5701 ccaggaaaga tatgtgggtg agatacgtgc atcaatatat tcccatgccc atttcagttg
     5761 ttcggtattc tttttaagcc atttggcttc gtttctcttt tcgagtccca gccagttttg
     5821 atgcattatt gagataatcc gatctgcttc cccataaagg attaacgcat taatccattt
     5881 ccctataaca ttcagtcgtg tttcatgact tgaaggaata attgtcatgg ggttatcctt
     5941 cataacgatc acccccttca aggcagaatt gaactcagat tcattattaa gtttaagttt
     6001 ttcgggaact gtatgagaca taattaacca gaaccagaat gctgcaattt cattttttct
     6061 gatccattca ggtatgtgat tatctgccag attattatca ttcaataaat gctcagttgt
     6121 tttatttttc cagtcatatc tattcacgtt ataaccgcct ttatattgtt tcatctgatt
     6181 acgcctcaat cgttttcttt tctggtgatt ttcaccatca catgtcagtt gtatatgcaa
     6241 gggaatcaat aaaaatatca ccctgcaagc acttcaaagc tgattatggg gatattctag
     6301 aactattcac aaaactattc cagacatatc aagaactatt tcttgatcta ttcataataa
     6361 aataaaacat gctatcaact acggatcata tcctccttag gtgtgtcgtt gtactgttcg
     6421 actgttcttg tggtcagatt aacaatctga agagtaaaag cgacagtaat ctgtctgtta
     6481 tagtggcgat aaaaacactg actgcgcatc ctgtatgtga aatcctgtca tgtagagcag
     6541 taagcaatga cagacatgca tgttatatgt gaaggggccg ggagacgaca taatcagtat
     6601 ggctgtcatt ctggttgtaa aacgcgatca ctttccgggg gtaaaagatg atatggaggt
     6661 tgacaaacgg tttgcttcgg attgtctttt ctggggcctg aagtacgggg gcagaaaaat
     6721 aattccagag taaatctgct ctgtttttag gacagaaaac tccatgctgg ctgtaaaagt
     6781 gaaaaattaa cacctagtat gtatggaagt ctgaagatat tgagaatgtc ggtatgatac
     6841 tatatacaca tattttacta agtattttta ctaagcattt cagtaagtgt tatacacgta
     6901 ttttctacta agtgttttac cagacataca cgtgttttca taaacagccc tataagtgtt
     6961 ttcattaagc attgctgtac acattgcttt gtctgaaaca catccagttt agtaaatctt
     7021 tacgttagct aaaatcagtt taaagctgct gtaaaacctt tttgtttaag caatcaatct
     7081 gctattcgat tttactgatg ttgcgtttaa ccttttctga gtttttgtgt ttttagtcgt
     7141 taacctgaga caagtgtctt tacgtaaaaa cgcaaatccg actgagataa cctggtgtga
     7201 tttacggtgt gcttgcgacg attttgttaa atggttctat ggatgatttt gtgacgatga
     7261 gattgatgag atgattaact ggccctggtg ttttttctca gtgcaaggtg attatcttta
     7321 tcataaggct cttttcagaa aaagcgtgat gattggttct ggtgttttga ttcagatgag
     7381 atttacgtta tcgatggtta atcgttttta tggtgtgttg aaaagtaacg ttttcaggat
     7441 aagaaaatcg attctgatat tggtgaactt tgttcttgtg gcagagttat ctgtaaacag
     7501 gttttaagtg cgctattcgg ctctgtgatt gagttgtgtg gttaagctgt aggttttggt
     7561 gttgttgttg atttgcattg ccaggaagcg ttctggttgc gtttctggtg ttttgttcga
     7621 atgttctgtg gatccttttc ccttcaggct tttctcgtcg tccatgtcgt ttttaaatcc
     7681 attcatgctg ttgttgcgtg ttttgctgct gtgtgcagga ttatcttttc gttccgggat
     7741 ggtttgttcc gtttcaatca ctcttaatca gagagttgta gcattgctca atgccggatg
     7801 ccgtaaagtt ttaccgcgtt gatgaatgat gtgaagtcaa tccaaatcaa cggagatctc
     7861 tcatcatgaa tcaaccaata cacaatgctt actggttatc ccgttttgac atccttctcg
     7921 acagtgccct ggcgcagcac cgtgccgtct cgttaatccg agtggattta cgtttccctg
     7981 agtatatgcc tgtcaccatc atggatcccg atccggattc agcggtgatt tctcgttttt
     8041 ttgcatccct gaaagccaaa attcaggctt accagcggaa aaaaagatgt gccaatcagc
     8101 gtgtgcatgc aaccagcctg cgttatttct ggtgccggga gttcggcaag atgtatggca
     8161 ggaaacatta tcacgtgata ttactgctca acaaagatac ctggtgctcg atcggggatt
     8221 tcagcgaacc gtcttcgctg gcgacgatga tccaggaggc atggtgcagc gccctgcatc
     8281 ttgagccctg gcaaggcgat ggcctggtcc atttttccag gtggacgcct tcccgtaaac
     8341 cagcatcgtc tgatgctcgc ccttcttccg atgacacgcc tttgtcgggt ggatgttctg
     8401 atacctggaa ggcttcagac aaaaagccgg gtgaagccgc tgttctctgg atcaagcgtg
     8461 gtgatgtggg agcgctgcag aaagcccgga acagagccag ttatctcgta aaatatgaaa
     8521 cgaagttgca taacggttcc gggcaacgta attatggttg cagtcgtggg ccgggacgtc
     8581 tactggatgg caggagaagc ctgtaaaacg gcatccggag cctgagctta tgccacagta
     8641 agggcgtcag ccaacgccct tagctcgttt tctgaaaaag tcgtcctgaa gtcatgtatc
     8701 tgcttccatt ctcatgttca tatttcacct tacgctttca ctatctgtta ttggaagtac
     8761 cgatttattt tggtgctgct cacttacggt cattcacgga agaggatggt ggtgaaccgt
     8821 acgatcctgg ctgctggcga gggaaggttc aggactggga tggagcaggt ttagtggtgg
     8881 aagatgagtc ggaatttctg actggtcagt gaggtgatga tctggtgtaa ctcaatctgc
     8941 tagggtaaac tctccttgct tttgcgcaca agcagtgggc cctggttcgc ttatgtcctg
     9001 catctacaag cgaatgactg accggagact cccatatctg gtcagtcgcc catttttatt
     9061 ttctaccaaa tcagacaagc ataccgtaat aaataacgcc agtcccctgt cgctctcgtg
     9121 tgctctactg gaatgttgtt gatggagata tccagatgaa agagcttaca tcttcgacag
     9181 gactataact tatgtagtaa agagttcccc ggagaaaggg gctgttctgc tgttctcatc
     9241 aagtctggca tgttcaggaa attattcgaa aatattaact gaatgagcga aatgtgattt
     9301 accttctttt ctgttcattg taaatttcct ctttgctaac gcaaatcatt atcattacgc
     9361 ctttggcaaa gggaaattta tgaagaacaa atatatcatt gctccgggca ttgccgtgat
     9421 gtgttctgca gttatatcat caggttatgc cagttctgat aaaaaagaag atacgcttgt
     9481 tgttactgcc tccgggttca ctcagcagct cagaaatgcc ccggccagtg tctcagtcat
     9541 tacttcagaa caactgcaaa aaaaaccggt ttcagatctg gtcgatgcag taaaagatgt
     9601 tgaagggatt agtatcactg gtgggaatga aaaaccggat atcagtatac gtggtctaag
     9661 tggcgattac acgctgattc tggtcgatgg acgacgtcag agcggtcggg aatccagacc
     9721 aaacggcagc ggcggttttg aagccggatt tatccctcct gtggaagcaa ttgaacgcat
     9781 tgaagtgatc cgtggcccta tgtcttccct gtatggttct gatgccatcg gaggggtcat
     9841 taatatcata accaaaccag ttaataacca aacatgggat ggcgtacttg gacttggggg
     9901 gattattcag gaacatggga aatttggtaa ctcaaccaca aatgacttct atctgtcagg
     9961 cccattgatt aaggataaac ttggtcttca gctatatgga ggaatgaact atcgcaagga
    10021 agatagtatc tctcagggaa caccggcaaa agataataag aatataacgg caacgctcca
    10081 gtttactccg actgaaagcc agaagtttgt ttttgaatat ggaaaaaata accaggtgca
    10141 tacattaaca cctggtgagt ctctcgatgc ctggactatg cggggaaatc ttaaacaacc
    10201 aaacagtaaa agagaaacgc ataattcacg tagtcactgg gtagcagcat ggaatgccca
    10261 gggcgaaata ctgcatcctg aaattgctgt ttatcaggag aaagttattc gtgaggttaa
    10321 atcaggtaaa aaagataaat ataatcattg ggatcttaat tacgagtcaa gaaaaccgga
    10381 aataaccaac acaatcatag atgcaaaagt gacggcattt ctgccggaaa atgtactgac
    10441 catcggaggt caatttcagc atgcagagct ccgtgatgac tcagccacgg gtaaaaaaac
    10501 gacagaaaca cagtctgttt caattaaaca gaaagctgtt tttatagaaa atgaatatgc
    10561 agcaacggat tctctcgccc tgactggagg actgcgtctc gataatcatg aaatctatgg
    10621 cagttactgg aatccaagat tgtacgctgt ttataacctg accgataatc tcacactcaa
    10681 aggggggatc gcaaaagcat ttcgggctcc ttcaattcgt gaggtgagtc ctggatttgg
    10741 aacactgacg cagggtggtg cctctattat gtatggaaac agggacctga aaccggagac
    10801 cagtgtaacc gaagagatcg gtattattta tagtaatgat agtggttttt cggcgagcgc
    10861 gacgctgttt aatactgatt ttaaaaataa gttgaccagt tacgatatag gtacaaaaga
    10921 tccagtcacc gggttaaaca cttttattta tgataatgta ggtgaggcaa atatcagagg
    10981 ggtggagctt gcaactcaga ttcctgtgta tgataaatgg catgtatctg caaactatac
    11041 atttactgac tctcgtcgaa aaagtgatga cgaaagtctc aatggcaagt cgctgaaagg
    11101 ggaacctctg gaaagaactc ccagacatgc agccaatgca aaactggaat gggattacac
    11161 tcaggatatt acattttatt catctctgaa ttatacggga aaacaaatct gggcagcaca
    11221 aagaaatggt gctaaggttc cccgcgttcg taatggattc acatctatgg atattggtct
    11281 aaattaccag attctgccag acacgctgat taattttgcc gttcttaacg tcacagacag
    11341 aaagagcgag gatatcgata ccattgatgg taactggcag gtcgatgaag gacgccgtta
    11401 ttgggctaat gtaagagtat ccttctgaga gtatataaag acaacctgag gccgtgagcc
    11461 tcaggtacca gtgtagtaat cagtaatgtc agtttccagg ttgtaatgct tttaatcttg
    11521 attaaaataa tcttaataat ttaaacttgt taaatatcga tgattttaat ttgagagcgt
    11581 tcagaattct gtgtcacgtt aaaaattcta cagcgtcatc acattattca gccgcccttc
    11641 aaaataaatg gctaactgcg acaatgtcaa attccagctt tggatgggca ttgtccattt
    11701 ctcctgtgcg ttcatcagtc ccagataaag tagcttcaac aggctgtttt cattcgggaa
    11761 agcaccttta ggttttggtc agtttcctga actggcggtg gactgattct atcgcgttgg
    11821 ttgtgtagat caccttgcgg atattcgccg gatagcggaa gtacgctgac agattttccc
    11881 tcttgcggcg ccaggactgg agcaccaccg gatattgctg cccccgtttc gcttccagct
    11941 catccagcgc cgtttccgct gcttcttttg acaccgcacg atacactggc ttcaggtcgg
    12001 ccatgaacgc tttatggtgt tttgacgcca catatttaat cgagttacgt atctggtgaa
    12061 tgacgcacaa ctgtacctct gtctgcggat aaatgctgtt tatcgcttca gggaagccgg
    12121 tcagcccatc cacgcaggca atcagaatgt cttcaacgcc acggttttgc agatcactta
    12181 atactgacag ccagaagtta gcgccttcgc tttccgacaa atacaggccc aggacttctt
    12241 ttttgccttc cagattcagc gccagtacgg tataaactgc cttgctctga taacacccat
    12301 cttcgcggat tttatagtga atggcgtcca gccagacgaa gggataaacc ttttccagcg
    12361 ggcgctgctg ccactgtttt aactcaggaa tgactttatc ggtaaccgca ctgatggtag
    12421 tcgtggaaac actgaaggca tataaatctt cgatctcccg gctgatatcc tggtagctca
    12481 tccccagcgc gaacaggcga atgatcttgc gctcgagctc atcagacaac gtggtctggt
    12541 gcttcttcac cagttggggt tcaaaataac cattacgatc acgtggagtt gccagctcaa
    12601 agctgccagt cggggcttta atggtctttt ttccggaacc atttttacga ttgacttcaa
    12661 catcctgagc cagatgggag tcgagttcag cggccagagc ggtttctgtt aactgtttga
    12721 ttaatggcgt taggatacca tccttgccgg ttaatgcttg gccggattga accgccccgg
    12781 gaatcctgga gactaaattc cctgagaaag aggtaaacag gatgactaaa aatacacgtt
    12841 tttcccccga agtccgtcaa cgggcagttc gtatggttct ggaaagtcag gacgaatatg
    12901 actcacaaag ggcggcaatt tgttccattg ctccaaagac tggctgtacg ccagagactc
    12961 tgcgtgtctg g
//
LOCUS       DAOFSV010000129        12494 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.129, whole genome shotgun sequence.
ACCESSION   DAOFSV010000129 DAOFSV010000000
VERSION     DAOFSV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.129"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            67..450
                     /locus_tag="QBH27_003718"
     CDS             67..450
                     /locus_tag="QBH27_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015982.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(514..957)
                     /locus_tag="QBH27_003719"
     CDS             complement(514..957)
                     /locus_tag="QBH27_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDQ7015983.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPIGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTTVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="QBH27_003720"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="QBH27_003720"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDQ7015984.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="QBH27_003721"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="QBH27_003721"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDQ7015985.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGRLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="QBH27_003722"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="QBH27_003722"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDQ7015986.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5372..7096
                     /gene="aceK"
                     /locus_tag="QBH27_003723"
     CDS             5372..7096
                     /gene="aceK"
                     /locus_tag="QBH27_003723"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDQ7015987.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="QBH27_003724"
     CDS             complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="QBH27_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDQ7015988.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8138..11821
                     /gene="metH"
                     /locus_tag="QBH27_003725"
     CDS             8138..11821
                     /gene="metH"
                     /locus_tag="QBH27_003725"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDQ7015989.1"
                     /translation="MSSKVEQLCAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVISAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     HRKGMSVTEVERWLAPNLGYDAE"
     gene            12242..>12494
                     /locus_tag="QBH27_003726"
     CDS             12242..>12494
                     /locus_tag="QBH27_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7015990.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQ"
BASE COUNT         3060 a         3143 c         3318 g         2973 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcatgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt cccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acagtggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaatccaa tcggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggcggtaca ggccgcgcta
     1561 aatatcctct acggcattcc taagcaaact cgcaccgaca aactctctgg cgtttatgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattacac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgaatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccggactaga cccggaagta ccgtataact atttcccgca caatgatccg
     1921 caaaacaaac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag taagtgaata aattttattt atattgttat
     2161 caacaagtta acatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaacgaaaag cgcacaacga tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttc acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgcattcag cagcagcagg atatcgataa cggaaggttg cctgatttca tttcggaaac
     2521 agcttccatt cgtgatgctg actggaaaat tcgcgggatt cctgcggact tagaagatcg
     2581 tcgcgtagag attaccggac ctgtagagcg caagatggtg atcaacgcgc tcaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgatgggcaa attaacctgc gcgatgcagt taacggcacc atcagctata ccaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc agcggttttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgaggca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaactatca ggcactgttg gcaaagggca gcggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacat tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgcggt gcttttgcga tgggcggcat
     3301 ggcggcgttt attccgagca aagatgaaga gcgcaataac caggtgctcg acaaagtaaa
     3361 agcggataaa tcgctggaag ccaataacgg tcacgatggc acatggatcg ctcacccggg
     3421 ccttgctgat acggcaatgg cggtattcaa cgatattctc ggctcccgca aaaaccagct
     3481 tgaggtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtga
     3541 tggtgaacgc accgaagaag gtatgcgtgc caacattcgc gtggctgtgc agtacatcga
     3601 agcgtggatc tctggcaatg gctgtgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgactaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccaggggcgt tttgacgatg ccgcacgctt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcgcccg
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atccggaatg caccctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggcggtcag gcgttgcaac aggcgaaagc gggtattgaa
     4201 gcagtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagctg tggtagagcg gatcaacaat
     4321 actttccgcc gtgcggatca gatccaatgg tccgcgggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgtcgcc gatgcggagg ccggttttgg cggtgtactg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg catcagtgaa gaaatgtggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgatgtgac gggcgttcca
     4621 accctgctgg ttgctcgtac cgatgccgat gcggcggatc tgattacctc cgattgcgac
     4681 ccatatgaca gcgaatttat tactggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcgggtattg agcaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggatctggaa ctggcgcgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggtatccac agcatgtggt tcaacatgtt tgacctggca
     5041 aacgcctatg cccagggtga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcacc agcaggaagt ggggacaggt
     5161 tatttcgata aagtgacgac cattattcag ggcggcacgt cttcagtcac cgcgctgacc
     5221 ggctccaccg aagaatcgca gttctaagca actgataaag ttgatgattg taggccggat
     5281 aaggcgttca cgccgcatcc ggcaatcaac gcctgatgcg acgatttgcg cgtcttatcc
     5341 ggcaattctc tgctcctgat gagggcgcta aatgccgcgt ggcctggaat tactgattgc
     5401 acaaaccatt ctgcaaggct tcgacgctca gtatggtcga ttcctcgaag tgacttccgg
     5461 ggcgcagcag cgtttcgaac aagccgactg gcacgctgtc cagcaagcga tgaaaaaccg
     5521 tatccatctt tacgatcatc acgtgggtct ggttgtggag caactgcgct gcattactaa
     5581 cggccaaagc acggacgcgg catttttact gcgcgttaaa gagcattaca cccgactgtt
     5641 gccggattac ccgcgcttcg agattgcgga gagctttttt aactccgtgt actgtcggtt
     5701 atttgaccac cgctcgctta ctcccgagcg gctttttatc tttagctccc agccagagcg
     5761 ccgttttcgt accattcccc gcccgctggc gaaagacttt caccccgatc acggctggga
     5821 atctctgctg atgcgcgtta tcagcgacct gccgttgcgt ctgcgctggc agaataaaag
     5881 ccgtgacatt cattacatcg ttcgccatct aacggaaacg ctggggacag acaacctcgc
     5941 ggaaagtcat ttacaggtgg cgaacgaact gttttaccgc aataaagccg cctggctggt
     6001 gggcaaactg atcacgcctt ccggcacatt gccatttttg ctgccgatcc accagacgga
     6061 cgacggcgag ttatttattg atacctgtct gacgacgacc gccgaagcga gcatcgtctt
     6121 tggctttgcg cgttcttatt ttatggttta cgcgccgctg cccgcagcgc tggtcgagtg
     6181 gctacgggaa attctgccag gtaaaaccac cgctgaattg tatatggcta tcggctgcca
     6241 gaagcacgcc aaaaccgaaa gctaccgcga atatctcgtt tatctacagg gctgtaatga
     6301 gcagttcatt gaagcgccgg gtattcgtgg aatggtgatg ttggtgttca cgctgccggg
     6361 ctttgaccgg gtatttaaag tgatcaaaga caagttcgcg ccgcagaaag agatgtctgc
     6421 cgctcacgtt cgcgcctgct accaactggt gaaagagcac gatcgcgtgg gccgaatggc
     6481 ggacacccag gagtttgaaa actttgtgct ggagaagcgg catatttccc cggcattaat
     6541 ggcattactg ctccaggaag cagcggaaaa aatcaccgat ctcggcgaac aaattgtgat
     6601 tcgccatctt tatattgagc ggcggatggt gccgctcaat atctggctgg aacaagtgga
     6661 aggtcagcag ttgcgcgatg ccattgaaga atacggtaac gctattcgcc agcttgccgc
     6721 tgctaacatt ttccctggcg acatgctgtt taaaaacttc ggtgtcaccc gtcacgggcg
     6781 tgtggtgttt tatgattacg atgaaatttg ctacatgacg gaagtgaact tccgcgacat
     6841 cccgccgccg cgctacccgg aggacgaact tgccagcgaa ccgtggtaca gcgtctcgcc
     6901 gggcgatgtt ttcccggaag agttccgtca ctggttatgt gccgacccgc gtattggtcc
     6961 gctatttgaa gagatgcacg ccgacttgtt ccgcgctgat tactggcgcg cactacaaaa
     7021 ccgtatacgt gaagggcatg tggaagatgt ttatgcgtat cggcgcaggc agcggtttag
     7081 cgttcgattc gtataaatca gaggcgaaaa agtcagcgta ttccaccgta tgccagcgtc
     7141 acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc atcggtaata
     7201 cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc atcgaaaata
     7261 cacgctgcaa ggcaacgcag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt
     7321 ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt tgcgtgggta
     7381 taggcatgta accctttgcg gtgcagtaac ttcgtcacct gttcttcgct cagttgggcc
     7441 agaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac
     7501 attcgcatca ggtgagtaca ctgtacctgg tcgataataa tcgcttcgtg attgctttga
     7561 tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttagattgcg caggataggg
     7621 tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcgtgt
     7681 gcgccgatcg cccagtgacc cagttcgccg acctgacgga caaagccctg ttgctgcatc
     7741 gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgctgtgc cagttccgtg
     7801 agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa
     7861 gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgttttgcg
     7921 ggaatgggtg cgaccatgac agtctccttt tttctgtatc gtggaaatca ttttcatttt
     7981 tattatcagc gaatgcaata gttgctgaac agatccgatg agttaatgtt gaacaaatct
     8041 catgttgcgt ggtggtcgct tttaccacag atgtgtttat gccagtatgg tttgttgaat
     8101 ttttattaaa tctgggttaa gcgtgtcggg agcaagtgtg agcagcaaag tggaacaact
     8161 gtgtgcgcag ttaaatgaac gtattctggt gctggacggc ggtatgggca ccatgatcca
     8221 gagctatcga ctgaacgaag ccgattttcg tggtgaacgc tttgccgact ggccatgcga
     8281 tctcaaaggc aacaacgacc tgctggtgct cagcaaaccg gaagtgatct ccgccatcca
     8341 caacgcttac tttgaagcgg gtgcggatat catcgaaacc aacaccttca actccacgac
     8401 tattgcgatg gcggattacc agatggaatc cctgtcggcg gaaatcaact ttgcggcggc
     8461 gaaactggcg cgcgcctgtg ccgacgagtg gactgcccgc acgccggaga aaccgcgcta
     8521 cgttgccggt gttctcggcc cgaccaaccg cacggcgtct atttctccgg acgtcaacga
     8581 tccggcattc cgcaacatca cttttgacca gctggtggcg gcttatcgag aatccaccaa
     8641 agcgctggtg gaaggtggcg cggacctgat cctgattgaa accgttttcg acacccttaa
     8701 cgccaaagcg gcggtctttg cggtgaaaac ggagtttgaa gcgctgggcg ttgaactgcc
     8761 gattatgatc tccggcacca tcaccgacgc ctccgggcgc acgctctccg ggcagaccac
     8821 tgaagcgttt tacaactccc tgcgccacgc cgaagcgctg acctttggcc tgaactgtgc
     8881 gctggggcca gacgaactgc gccagtacgt acaggaactg tcgcgtattg cggaatgcta
     8941 tgtcaccgcc cacccgaacg ccgggctgcc caacgccttt ggtgaatacg atctcgacgc
     9001 cgacacgatg gcaaaacaga tacgtgaatg ggcagaagcg ggctttctca atatcgtcgg
     9061 cggctgctgc ggcaccacgc cacaacatat tgcagcgatg agtcgtgcag tagaaggatt
     9121 agcgccgcgc aaactaccgg aaattcccgt agcctgccgt ttgtccggcc tggagccgct
     9181 gaacattggc gaagatagcc tgtttgtgaa cgtgggtgaa cgcaccaacg tcaccggttc
     9241 cgctaagttc aagcgcctga tcaaagaaga gaaatacagc gaggcgctgg atgtggcgcg
     9301 tcagcaggtg gaaaacggtg cgcagattat cgatatcaac atggatgaag ggatgctcga
     9361 tgccgaagcg gcgatggtgc gttttctcaa tctgattgcc ggtgaaccgg atatcgctcg
     9421 tgtgccgata atgatcgact cctcgaaatg ggacgttatt gaaaaaggtc tgaagtgtat
     9481 ccagggcaaa ggcattgtta actctatctc gatgaaagag ggcgtcgacg cctttatcca
     9541 tcacgcgaaa ctgttgcgtc gctacggtgc ggcagtagtg gtaatggctt ttgacgaaca
     9601 gggccaggcc gatacccgcg cacggaaaat cgagatttgc cgtcgggcgt acaaaatcct
     9661 caccgaagag gttggcttcc cggctgaaga tatcatcttc gacccgaaca tcttcgcggt
     9721 cgcaactggc attgaagagc acaacaatta cgcacaggac tttatcggcg cgtgtgaaga
     9781 catcaaacgt gaactgccgc acgcactgat ttccggcggc gtttctaacg tgtcgttctc
     9841 gtttcgtggt aacgatcctg tgcgcgaagc cattcacgcg gtgttcctct actacgctat
     9901 tcgcaatggc atggatatgg ggatcgtcaa cgccgggcaa ctggcgattt acgacgacct
     9961 gcctaccgag ctacgcgacg cggtggagga tgtgatcctc aaccgccgcg acgatggcac
    10021 cgagcgttta ctcgagcttg ccgagaaata tcgcggcagc aaaaccgacg acaccgctaa
    10081 cacccagcag gcggagtggc gctcgtggga agtgaataaa cgtctggaat actcgctggt
    10141 caaaggcatt accgagttta tcgagcagga taccgaagaa gcccgccagc aggctacgcg
    10201 cccgattgaa gtgattgaag gcccgttgat ggacggcatg aacgtggtcg gcgacctgtt
    10261 tggcgagggc aaaatgttcc tgccgcaggt ggtgaaatcg gcgcgcgtaa tgaagcaggc
    10321 ggtggcgtat ctcgagccgt ttattgaagc cagcaaagag cagggtaaaa ccaacggcaa
    10381 gatggtgatc gccacagtga agggcgatgt ccacgacatc ggtaaaaata tcgttggcgt
    10441 ggtgctgcaa tgtaacaact acgaaattgt cgatctcggc gtgatggtgc ctgcggaaaa
    10501 aattctccgt accgctaaag aagtgaatgc tgatttgatt ggcctttcgg ggctgatcac
    10561 gccgtcgctg gacgagatgg ttaacgtggc gaaagagatg gagcgccagg gcttcaccat
    10621 tccgctattg attggcggcg cgaccacctc aaaagcgcac acggcggtga aaatcgagca
    10681 gaactacagc ggcccgacgg tgtatgtgca gaacgcatcg cgcaccgttg gtgtggtggc
    10741 ggcgctgctt tccgacactc agcgcgatga tttcgttgct cgtacccgca aggagtacga
    10801 aaccgtgcgt attcagcacg gacgaaagaa accacgcaca ccaccggtca cgctggaagc
    10861 ggcgcgtgat aacgacttcg cttttgactg gcaggcttac acgccgccgg tggcgcaccg
    10921 tctcggtgtg caggaagtcg aagccagcat cgaaacgctg cgtaattaca tcgactggac
    10981 gccgttcttt atgacctggt cgctggccgg gaagtatccg cgcattctgg aagatgaagt
    11041 ggtaggtgtt gaggcgcagc ggctgtttaa agacgccaac gacatgctgg ataaattaag
    11101 cgccgaaaaa atgctgaacc cgcgtggtgt ggtgggcctg ttcccggcaa accgtgtggg
    11161 cgatgacatt gaaatctacc gtgacgaaac gcgtacccat gtgattaatg tcagccacca
    11221 tctgcgccaa cagaccgaaa aaaccggctt cgctaactac tgtctcgctg acttcgttgc
    11281 gccgaaggtt tccggcaaag cggattacat cggcgcattt gccgtgactg gcgggctgga
    11341 agaggacgca ctggctgatg cctttgaagc acagcatgat gattacaaca aaatcatggt
    11401 gaaagcgctt gccgaccgtc tggcggaagc ctttgcggag tatctccatg agcgtgtgcg
    11461 taaagtctac tggggctatg cgccgaacga gaacctcagc aacgaagagc tgatccgcga
    11521 aaactaccag ggcatccgtc cggcaccggg ctaccctgcc tgcccggaac atacggaaaa
    11581 agccacaatc tgggagctgc tggaagtgga aaaacatacc ggtatgaaac tcacggaatc
    11641 tttcgccatg tggccgggtg catcggtttc gggttggtac ttcagtcatc cagacagcaa
    11701 gtattacgcg gtggcgcaaa ttcagcgcga tcaggttgaa gactatgccc accgtaaagg
    11761 aatgagcgtt accgaagttg agcgttggtt ggcaccgaat ctggggtatg atgccgagta
    11821 agtgataaca ccggggtatt tgccccggtt tttaatgtca taaataatgg attattgaat
    11881 atgataaaaa ttgatgaaat agattttttc gctttcgaag gatgttcaga gtccgaagtt
    11941 actgaactat taggaaaaaa ccaacaggtt ataatgattg gataattaat ataaaacagg
    12001 tgtggagtgg gtattttatg atggtcgtta ttggttagat tttcgtaaat ccaaagaggg
    12061 tataaggtgt atctcatgca gtattctgta tcacgataaa agtaaagggc gtatcgatgt
    12121 cttatttagt gaaggtatag ctgacagagg tttaaaaaat acgggtggag tgttaatcca
    12181 aaaacgcccg caattataat aattcctggt aatatttgtt ttcgtaaaaa ggatttactt
    12241 tatgcaacgt caatataaaa aatacattct tcttttttgg ggtgttatgg atgttatcgc
    12301 gatagccaca tatctgttct actcgcttgg taatggtcgt atcccttttt attccgatat
    12361 cgttcattca ctttctttac ttaaggatat cggtgttgaa ggcggttttt acgcttatgc
    12421 agtcgccact gttacgctac aaatagtatt attgttctct ttattctttt ctgcgcaatg
    12481 ttttttaaga caaa
//
LOCUS       DAOFSV010000130        12419 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.130, whole genome shotgun sequence.
ACCESSION   DAOFSV010000130 DAOFSV010000000
VERSION     DAOFSV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.130"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(437..2470)
                     /gene="betT"
                     /locus_tag="QBH27_003727"
     CDS             complement(437..2470)
                     /gene="betT"
                     /locus_tag="QBH27_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDQ7015991.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2599..3186
                     /gene="betI"
                     /locus_tag="QBH27_003728"
     CDS             2599..3186
                     /gene="betI"
                     /locus_tag="QBH27_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDQ7015992.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3200..4672
                     /gene="betB"
                     /locus_tag="QBH27_003729"
     CDS             3200..4672
                     /gene="betB"
                     /locus_tag="QBH27_003729"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDQ7015993.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILIYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4686..6356
                     /gene="betA"
                     /locus_tag="QBH27_003730"
     CDS             4686..6356
                     /gene="betA"
                     /locus_tag="QBH27_003730"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDQ7015994.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFNIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            7441..8004
                     /gene="ipbA"
                     /locus_tag="QBH27_003731"
                     /gene_synonym="fimX"
     CDS             7441..8004
                     /gene="ipbA"
                     /locus_tag="QBH27_003731"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDQ7015995.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
     gene            8334..9128
                     /gene="hyxR"
                     /locus_tag="QBH27_003732"
     CDS             8334..9128
                     /gene="hyxR"
                     /locus_tag="QBH27_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDQ7015996.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLRTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSEQRKNISKKRPGLL"
     gene            9282..10043
                     /gene="hyxA"
                     /locus_tag="QBH27_003733"
     CDS             9282..10043
                     /gene="hyxA"
                     /locus_tag="QBH27_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDQ7015997.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYLDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDCYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            10085..11161
                     /locus_tag="QBH27_003734"
                     /pseudo
     CDS             10085..11161
                     /locus_tag="QBH27_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            11183..12376
                     /locus_tag="QBH27_003735"
     CDS             11183..12376
                     /locus_tag="QBH27_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ7015998.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         3390 a         2998 c         3065 g         2966 t
ORIGIN      
        1 cgtcattact ctatgctaat cccttaacaa cctgcttttt aagactgaat ccattcagca
       61 atagatagcc aacattataa caatgacaat aaaaacaata tttaaaacaa tcaataaaat
      121 atcagacaca gacaattaaa ccttaaacaa caaacggtta cattgatttt ggttgatttg
      181 cgctacgcgt ttcccatgaa taaagcgtca tttcaaatta gcaccaatat ttacattaaa
      241 taagactaag cttattgaac attcacaaca gtccaagcgt aggttttggg cttcaatagt
      301 tcttttcttt tttattatct gttgcctggc aatcatcaat cgatactaag ggcttaactg
      361 tacacagata taacgtatca agaggtaaga gaaaccgcgc catatgcaga catggcgcgg
      421 ttttatgcaa taacaatcac gcgtccggga acatcacgct atggcccggc gcttcacgat
      481 ggagatggat aaagttaagg tgccgctcgt actggtcgag aatatcggtg atcacctgct
      541 ctttgctgta gtccatcagg tcgttgccct ggctgccttc taacaggaag gtttccagcc
      601 ggtagtaggt cgatttcccg ctacgtgcgc ggtaggtaaa gcccggcacc gaatattgct
      661 gtggccaaat ctgatagaca aagttttgct cttcgcccat atgcaccaac aaatccagat
      721 gccccaactg ttgtccctct tccggcggca ggttttttag ctccacgtac gcgccgcgca
      781 accgcagttc ctgcgccact tcttccattg ccggataaca gaccgtctcc atcatctgtt
      841 tagtgtaacg cgtgcccgga taattcatca ggcgcgagag acgttttttc cagctcaggc
      901 gatcctgaag ccccagcggt cgcggtgcgg tatcgcggtt ggcactttca cggcggtaat
      961 cttctacctt cagagattta tacaaccccg ccatcacgaa gaagatcaca aagctgaacg
     1021 gcaggcccat aatcaccgtg gtgttttgca gcgcggatat cccgttggtc atcagcatgc
     1081 ctagcgtcag caggccaatc gccaccgacc agaagacgcg cagccagccg ggtgcgtcgc
     1141 tgttgatatc tttaagctgc gaggtgaaat tccccagcac cagcgcaccg gagtccgccg
     1201 aggtcacata aaatagcagg ccagtaatgg tggcgacgga ggcgctaaag gtaaacgccg
     1261 gatactgcgc cagcaggctg tagaagccgc gctccggatg gaccatcgct tcctcggcaa
     1321 atgccgcgcc gccgtggatg atttcataca gcgcgctatt gccgaatacc gagagccata
     1381 acagcgtgaa ggtaaacgga ataatcaacg tgcccagcac gaactggcga atggtacgcc
     1441 cacgcgagat acgcgccagg aacaagccga caaacggcga ccatgccacc caccatgccc
     1501 agaagaagag cgtccagtta ttcatccact caaccggacg gtcgaaggca aaactattga
     1561 gcgtcatgcc cataaagcga ttcacatagt cgccaacgtt cagcaccagt gcattaagca
     1621 ggaacgaggt gtcgcccata aacaacacga acagaatcaa tcccagcgcc agcgcaacgt
     1681 tgagttccga taacacgcga atgcccttat cgacaccgga tgtcaccgag atagtggcga
     1741 tgatcaccga caaggcgatc agtgccgctt tcgccgccat cgaatcggga atatcaaaca
     1801 gcacgctcaa gccatagtta agctgcacca caccgatacc gagcgtagtg gcaataccga
     1861 agatggtgcc gatcaccgca gcaatatcca ctgagtgacc tatcggcccg ttaatccgtt
     1921 taccgaagat cgggtacagc gccgagcgga tggtgagcgg caaattataa cgatagctaa
     1981 agtatccgag cgccatgccc atcagcgcat acatcgacca gccggttaag ccgtagtgaa
     2041 acagcgtcca gaccatcgcc tgacgcgcgg cctcaatcgt ctgtcccgcg ccttccggcg
     2101 gctgcatata ctgcgttacc ggttcggcta cggagaagaa catcaggtcg ataccgatcc
     2161 cggcagcaaa cagcatcgcc gcccaactca gcaaactgaa ttccggtttt gattgttctg
     2221 gcccgagctt caccgaacca aaacgcgaac aagcgataca gaccacaaag acaatataga
     2281 gcgttgccgc cagcagatag taccaaccga aggttttaga aacccagtcc agcgtgcggc
     2341 caatccacag ggccgagaag tcgcgaaaca ggatcgttgt cagggaaaac aacaaaatca
     2401 gtccggcgga agtgtaaaac accaccggat tgattttgtc cttttccctg ctgtgtgaaa
     2461 ggtctgtcat ccagtatccc cactgttatt gttaccattt aaaatcaaat tagtaacaat
     2521 taagacacat tttatattga acgtccaatc aataaccgct ttaatagata aacaacgctg
     2581 atgaatggag tggcgaaaat gcccaaattg ggaatgcagt cgatccggcg cagacaactg
     2641 atcgacgcca cgctggaagc aataaatgaa gtgggcatgc acgatgcaac gatcgcgcag
     2701 atcgcccgcc gtgcaggcgt ttctacgggg atcatcagcc actatttcag ggacaaaaat
     2761 ggtctgctgg aagcaaccat gcgcgatatc accagtcagc tgcgtgacgc ggttttgaat
     2821 cgattacatg cacttccgca gggcagtgca gagctgcgat tacaggcgat tgttggcgga
     2881 aacttcgatg aaacccaggt gagcagtgcg gcgatgaaag cctggctggc cttctgggcc
     2941 agcagtatgc atcagccgat gctttaccgt ttgcagcagg tcagtagccg ccgcttattg
     3001 tcgaatctgg tgagcgaatt tcgccgcgag ttaccgcgcc aacaggcaca ggaagcgggc
     3061 tacggccttg ccgcgctaat tgacggatta tggctgcgcg cggcactgag cggcaaaccg
     3121 ctggataaac ctctcgcaca ctcgctgacc cgtcacttta ttactcagca tttacccacc
     3181 gattaaccga ggagacgtga tgtcccgaat ggcagaacag cagctttata tacatggtgg
     3241 ttatacctcc gccaccagcg gtcgcacctt cgagaccatt aacccggcca acggtaacgt
     3301 gctggcgacc gtgcaggccg ccgggcgtga ggatgtcgat cgcgccgtga agagtgccca
     3361 acaagggcaa aaaatctggg cggcgatgac cgctatggag cgctcgcgta ttctgcgtcg
     3421 ggccgttgat attctgcgtg aacgcaatga cgaactcgca aaactggaaa cccttgacac
     3481 cggaaaagca tattcggaaa cctcaaccgt cgatatcgtt accggtgcgg acgtgctgga
     3541 gtactatgcc gggctgatcc cggcgctgga aggcagccag atcccgttgc gtgagacgtc
     3601 atttgtttat acccgccgcg aaccgctggg tgtggtggcg gggattggcg catggaacta
     3661 cccgatccag attgctttgt ggaaatccgc cccagcgctg gcggcaggca atgcaatgat
     3721 tttcaaaccg agcgaagtca ccccgcttac cgcgttaaag ctggccgaaa tttacagcga
     3781 agcgggcctg ccggacggcg tatttaacgt gttgccgggc gtgggcgcgg agaccggaca
     3841 atatctgacc gatcatccgg gcattgccaa agtgtcattt accggcggtg tcgccagcgg
     3901 caaaaaagtg atggctaact cggcagcttc ctccctgaaa gaagtgacca tggagctggg
     3961 cggtaaatca ccgctgatcg ttttcgacga tgcggatctc gatctcgccg ccgatatcgc
     4021 catgatggcg aatttcttca gctccggtca ggtgtgtacc aacggcactc gcgtcttcgt
     4081 tccgacgaaa tgcaaagccg catttgaaca aaaggttctg gcgcgcgttg agcgcattcg
     4141 cgcgggcgac gttttcgatc cgcaaactaa cttcggcccg ctggtcagct tcccgcatcg
     4201 cgataacgtg ctgcgctata tcgccaaagg caaagaggaa ggcgcgcgcg tactgtgcgg
     4261 cggcaatgta ctgaaggggg atagcttcga taacggcgca tgggttgcac cgaccgtgtt
     4321 caccgattgc agcgacgata tgaccattgt gcgagaagag attttcgggc cagtgatgtc
     4381 cattctgatc tacgagtcgg aagacgaagt cattcgccgc gctaacgata ccgactacgg
     4441 tctggcggcg ggtatcgtga cggcggacct gaacctcgcg catcgcgtca ttcatcagct
     4501 ggaagcgggt atttgctgga tcaacacctg gggcgaatcc ccggcagaga tgcccgttgg
     4561 cggctacaaa cactccggca ttggtcgcga gaacggcgtg atgacgctcc agagttacac
     4621 ccaggtgaag tccatccagg ttgagatggc taaattccag tccatattct aaccaggagg
     4681 tttatttgca atttgactac atcattattg gtgccggctc agccggcaac gttctcgcta
     4741 cccgtctgac tgaagatccg aacacaaccg tgctgctgct tgaagcaggc ggcccggact
     4801 atcgctttga cttccgcacc cagatgcccg ccgcgctggc gttcccgcta cagggcaaac
     4861 gctacaactg ggcctatgag acggaacccg aaccgtttat gaacaaccgc cgcatggagt
     4921 gcggacgcgg caaaggtctg ggcggttcat cgctgatcaa cggtatgtgc tacatccgtg
     4981 gcaatgcgct ggatctcgat aactgggcac aagaacctgg tctggagaac tggagttatc
     5041 tcgactgcct gccctactac cgcaaggccg agactcgcga tgtgggcgag aacgattacc
     5101 acggtggcga tggcccggtg agcgtcatca cctccaaacc cggcgtcaat ccgctgtttg
     5161 aagcgatgat tgaagcgggc atgcaggcag gctacccacg cacggacgat ctcaacggct
     5221 atcagcaaga aggttttggc ccgatggatc gcaccgtcac gccgcatggc cgtcgcgcca
     5281 gcaccgcgcg tggctatctc gatcaggcca aatcgcgccc taacctgacc attcgtactc
     5341 acgccatgac cgatcacatc atttttgacg gcaaacgcgc ggtgggcgtc gagtggctgg
     5401 aaggcgacag cactattccg acccgcgcgg cggcgaataa agaagtgctg ttatgtgcag
     5461 gcgcgattgc ttcaccgcag atcctgcaac gctccggcgt cggcaacgct gaactgctgg
     5521 cggagtttaa tattccgctg gtgcatgaat tacccggcgt cggcgaaaat cttcaggatc
     5581 acctggagat gtatctgcaa tacgagtgca aagaaccggt ttccctctac cctgccctgc
     5641 agtggtggaa tcagccgaga atcggtgcgg agtggctgtt tggcggcacc ggcgttggtg
     5701 ccagcaacca ctttgaagca ggcggattta ttcgcagccg tgaggaattt gcgtggccga
     5761 atattcagta ccatttcctg ccagtggcga ttaactataa cggctcgaac gcagtgaaag
     5821 agcacggctt ccaatgccat gtcggatcga tgcgctcgcc aagccgtggg catgtgcgga
     5881 ttaaatcccg cgacccgcac cagcatccgg gaattctgtt taactacatg tcgcacgagc
     5941 aggactggca ggagttccgc gacgcaattc gcatcacacg cgagatcatg catcaacccg
     6001 cgctggatca ataccgtggc cgcgaaatca gccctggtgt cgaatgccag acggatgagc
     6061 agctcgatga gttcgtgcgt aaccacgccg aaaccgcgtt tcatccgtgc ggtacctgca
     6121 aaatgggtta tgacgagatg gctgtggttg acggcgaagg ccgcgtacac gggctggaag
     6181 gcctgcgcgt ggtggatgcg tcaattatgc cgcaaattat caccggcaat ttgaacgcca
     6241 cgacgattat gattggcgag aaaatagcgg atatgattcg tgggaaggaa gcgttgccga
     6301 ggagcacggc gggatatttt gtggcaaatg ggatgccggt gagagcgaaa aaatgagtcg
     6361 tgatgtgaac tgactcagga acctatcaga ttgagagtta ccgttctact atgaggaccg
     6421 aatccttata taaaacactg aggtaactct catgcttcat cgtgacaatc tcgccagcaa
     6481 attctaagct gacttgctca atctggctga tgaatgtgct atgcatacac agataaacta
     6541 cagatgaaat accactcagc ttaagaatta cgccgctaat gttcctggct atggttacct
     6601 gttcattccc gctcacggtt agcatatacg aaaagggttc ttgtaacgag tacaaaaata
     6661 agtcagacca aaaccacatg gagctttaaa cacgattcgt ataatttatt agcagtgatt
     6721 tatttttact cttctttaat taatcatcat gcttaaatat tttttagcac aagattatcg
     6781 cttcgctaac attttagttt acattaattt tcaattgata ataaaaagtt gttaataagt
     6841 tttttgcgga aaataaaaag tgttggaata ctctccatca aatgaatgca aaataaacaa
     6901 attgagatat acaaaaagta aaaatcattt aaatgaatat tttaaattta tgagcagaac
     6961 ccattaacaa tgatttactc acgaccagaa aagtaaagaa caccttgcaa taatatgtat
     7021 ttataaattc atatcattga tattaaacta atcttacttt ctaccctcca aatttatgca
     7081 tcaaaaaaaa caaaagtaaa atcaattaga tacacaacaa gacgatctca caggaaatga
     7141 tgttaaatat ctcaaaacta ataaaaatca ctaaaacatt agacgtaata cacatcccac
     7201 acgacctgtc actcatagtt ttaatattta ttttttcaca tcatagtaga taaaaatacg
     7261 cattaaaaat gacttgtaga aaagctgcac aataaatagt tcatatttaa tctgttgcgt
     7321 agaactacta ttcaaaaaat cttattaatg agataatgag attacatcga taacgttctg
     7381 attgcaggca tacttatctg ggtgtgcctc tctttttttt acaactgtac acggtggcgt
     7441 atgacgagaa aatatctcac acaggatgaa gtctacaggc tgatggatgc agcccagagc
     7501 atgtcctttc ctgaaagaaa tcgctgtcta attatgatgg ctttcattca cggttttagg
     7561 gccagtgaac ttcttgattt acgtttatcc gatattgatg catctggaaa acaacttaat
     7621 attcggcgta ttaaaaatgg tttctcgaca acacatcctc ttcttcctga tgaatataat
     7681 ttgatcaagt tgtggcttaa gcagaggaag ttaattgaaa acggagttga aggagactgg
     7741 ctttttctct cccggaaacg ccgccctatc agcagacaac attttttttc tatcattcgt
     7801 gaggctggaa aacgtgcagg attagctgta aaagcacatc ctcatatgtt acgtcatgcc
     7861 tgtggttttg ctctggctga caatggtgtc gatacccgac tacttcagga ttatctgggt
     7921 catcgaaata ttcagcatac tgtcagatac acagccagta atgctgctcg ttttaaaggg
     7981 gtgtggaaaa aaaagcctcg ttaaaacagt accttctgat tcaaaatgtc agattcctac
     8041 gttttcctca attcttccat aaaaaaacag cccattcaat taaataaaac acataacatg
     8101 ttgatatttc tagctaaagt aaaataaccc tatgatttga aatgattaat cacaaaggtc
     8161 ataccagtga ttaactttcg cccatatcgt taaataacac aaaattaaaa acaataaaaa
     8221 tcaaacaaat tagatttata ttccaagaaa attaaatatt caggctatta aaccaaacct
     8281 gacattttga atcaaaatgt caggttttgt agcaatggcg tggacgagtc tgaatgaaga
     8341 agcctctggt tattatcagt gcctgtcagt tcaccaggct ggcactggag tctctcatcc
     8401 caacggacag gtatatcgtg cgggtgtaca gcaacgtcac tacagaggtt gaattcgttt
     8461 taaagacctc ctgtggctac ctgttagctg atatcccttc gcttccttcg ggggagttgg
     8521 ttcttctggc ttgtctcgcc cgtttgcgga gtgatgccgg gcaatggcag gtctgcctga
     8581 ccggtgactc cgactggttc gataatgctc ctgctgcaag tctgtataac ggcttcccac
     8641 gcatcggcac catgctgaca gcgagtcgtt atcagaatca aattatccgc tggttactgc
     8701 gtcctttgcc cggcgatatg agcgatttct ggctcctgac tgcacgggag ttgagcgtgc
     8761 ttaagattct gatgcagggc atgagtttca gtgagattgc ccgttatgaa caacgcagtt
     8821 caaagaccct gcatgcgatt gccacacggg ctttaatgaa acttggactg agaacactga
     8881 gcgatttcag gctgctctat acgggatgcg gtgacagcag agtgaaacgg aatatacggc
     8941 tacatgcaaa atatatcgga caacattccg ctcatcgact cactcagtat gtgcagaaca
     9001 tggtatccgg aatcctttat ccagagaaag ttgccgttgg tgaatcaata aattctgacg
     9061 ccatttccgg aaaagtactt tccgaacaaa gaaaaaatat atctaaaaaa agaccaggat
     9121 tgttatgaca cagcagcgtt gttactacca cccactgcgg cgcacacgga ctcgctacag
     9181 tgtaggagcc aaaatatttc tactgttctg ggctgcagga ttgggaggca tgctgtacag
     9241 cctgtacagc gtgctggcgt actgacatct gtgagggaag gatgcgtttc gttcttttct
     9301 gtccctcagg aattgtacca gcgcagttcg cagctctatc aaccggttct gtcggcaatg
     9361 ttacctgcat aagaacagaa gaggagttac gcaataaatt gcggcacagg ccacagagtg
     9421 tcgtgatatc ggcagggcga cctgccgaat gcgctgaaat gtggttccga ttttatctgg
     9481 atcactcatt cgtggtggtg ttgtgcgtcg cgcccttttt tctgccaccg gatgtgagta
     9541 tttctggcgt tctcaaaaac ctgaggttac tgaaaccagg aatgtcagta gagcgtgtca
     9601 tcagtattgc aaatgcctct ggtgggtttt caggactgaa acatgcagaa aatttgccag
     9661 ttatggactg ttactctgtc tttatgaaag aggtgaataa ccgcacaaag acaatcgtca
     9721 tgtcagaacg cttcccggaa aagcaaaaga aggtgttgtc gttgcttctt gccggacaca
     9781 gctgggaata cagtgcgcag tttctcaaaa cggggataag acagatatgg cttgccgagc
     9841 agtcgctaaa aaagcgttgg gggataccgg acagcatgtc attgcgggaa gccctgctgc
     9901 tgagcagcaa taattttcac ggtgatggaa atgcattgga aacaacgaat gccatgacat
     9961 tgcgtgaaaa cggaaatacc aacaggtatt ccgtggtggt aaatgcaggg acgcaggctt
    10021 tatctctgca taaatataaa taagtgggaa attgaaatta gttaccagaa tggagaattt
    10081 cttcatgaaa aacagtaagg cattttaccg cagcgcatta gcgacagcta ttgttatggc
    10141 tctttctgca ccagcattcg ctgctgataa cgcggtatca actacaccgg ttacattgga
    10201 taaagataag acaactctgg aacaagatgt tgttattagt ggtactacac agattacagc
    10261 cgcaacaatt aatgcgaaag ataaagacct taatgttact ttgggtggtc atgatattac
    10321 cgccacatca acggtagacc aaggtttcgt tgaaggtgta aaagttagcg gtgacaaaaa
    10381 tgttgtgatt aatgctacag gctccaccat cacagctcaa ggtgaaggta cctatgtccg
    10441 aactgcaatg gtcattgatt caactggcga tgttgttgtt aatggcggta actttgttgc
    10501 aaaaaatgaa aaaagtagtg cgacaggtat atctctggaa gcgaccacgg gaaataattt
    10561 aacgctcaat ggcacaacca taaatgctca aggtaataag agttccagca acggctctac
    10621 ggcaattttt gctcaaaagg gtaatttgtt gcagggtttt gacggtgatg caaccgacaa
    10681 catcaccctt gctgactcaa atattattaa tggtgggatt gaaacaatag ttattgccaa
    10741 ggagaataag ggaattcata cagtcaacct gaatattaag gatggctcaa taattggggc
    10801 ggctaataat aaacaaacaa tttatgcctc tgcttcggca caaggcgcag gttcagcaac
    10861 gcaaaattta aatttgtctg ttgctgattc aaccatctac tctgatgtcc tggccctttc
    10921 tgaaagcgag aattcagccg gtaccacaac aaatgtaaat atgaacgttg cccgctctta
    10981 ctgggaaggt aatgcttata cgctcaatag cggcgataaa gcgggtagtg atctggatat
    11041 aaatctttcc gatagttcag tctggaaagg caaagtttca ggggcaggag atgccagtgt
    11101 atctctgcaa aacgggtctg tctggaatgt tacgggttcc tcaactgttg atgctctggc
    11161 agtaaaagac agtacggtta atatcacgaa ggctacagtc aatactggca cgtttgcttc
    11221 tcaaaacggc actctgattg ttgatgcctc ttctgaaaac actctggata tcagcggaaa
    11281 agcgagcggt gacttgagtg tttacagtgc gggttcattg gatcttatca atgaacaaac
    11341 ggcatttatt tctaccggca aagacagcac tctaaaagcc acaggcacaa cggaaggtgg
    11401 tctgtatcaa tatgacctga cacagggagc tgatggtaac ttttatttcg taaaaaacac
    11461 gcataaagca tccaacgcca gctccgtgat tcaggcaatg gcagctgctc cggctaacgt
    11521 cgctaatctg caggctgaca cgctctccgc ccgtcaggat gctgtccgtc tgagcgaaaa
    11581 tgacaagggt ggcgtatgga ttcagtactt tggcggtaaa cagaaacata ccaccgcggg
    11641 aaatgcatcc tatgacctgg atgtaaatgg tgtaatgctg ggtggtgata cccgcttcat
    11701 gactgaagat ggtagctggc tggccggtgt ggcgatgtct tctgcgaaag gtgacatgac
    11761 taccatgcag agcaaaggtg acactgaagg ttacagcttc cacgcttacc tgagccgcca
    11821 gtataacaac ggtatcttca ttgatactgc tgcacagttt ggtcactaca gcaacacggc
    11881 agatgttcgc ctgatgaatg gtggcggtac catcaaagct gactttaaca ccaatggttt
    11941 tggtgcgatg gttaaaggcg gttacacatg gaaagacggt aatggcctgt ttattcagcc
    12001 atatgccaaa ctgtctgctc tgactctgga aggtgtggat tatcaactca acggcgtgga
    12061 cgttcattct gacagctata actctgtgct gggtgaggcc ggtacgcgcg tgggttatga
    12121 cttcgctgtg ggcaacgcga ccgttaaacc ttatctgaat ctggccgcac tgaacgaatt
    12181 ctctgatggc aacaaagtcc gtctgggtga tgagtctgtc aatgccagca ttgacggtgc
    12241 agcattccgc gtgggtgcag gtgtacaggc tgatatcacc aaaaacatgg gagcatatgc
    12301 aagccttgac tacaccaaag gtgacgacat tgagaacccg ctacagggtg tagttggtat
    12361 caatgtgacc tggtaagaat aaagcagtcg gttcccctgt cgactgttat accccctgc
//
LOCUS       DAOFSV010000131        12293 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.131, whole genome shotgun sequence.
ACCESSION   DAOFSV010000131 DAOFSV010000000
VERSION     DAOFSV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.131"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..206)
                     /locus_tag="QBH27_003736"
     CDS             complement(<1..206)
                     /locus_tag="QBH27_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDQ7015999.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSG"
     gene            387..2018
                     /gene="dcuS"
                     /locus_tag="QBH27_003737"
     CDS             387..2018
                     /gene="dcuS"
                     /locus_tag="QBH27_003737"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDQ7016000.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKAQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2015..2734
                     /gene="dcuR"
                     /locus_tag="QBH27_003738"
     CDS             2015..2734
                     /gene="dcuR"
                     /locus_tag="QBH27_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDQ7016001.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3305..4645
                     /gene="dcuB"
                     /locus_tag="QBH27_003739"
     CDS             3305..4645
                     /gene="dcuB"
                     /locus_tag="QBH27_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDQ7016002.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4723..6369
                     /gene="fumB"
                     /locus_tag="QBH27_003740"
     CDS             4723..6369
                     /gene="fumB"
                     /locus_tag="QBH27_003740"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDQ7016003.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6492..7142
                     /locus_tag="QBH27_003741"
     CDS             6492..7142
                     /locus_tag="QBH27_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDQ7016004.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWLAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(7139..8494)
                     /gene="melA"
                     /locus_tag="QBH27_003742"
     CDS             complement(7139..8494)
                     /gene="melA"
                     /locus_tag="QBH27_003742"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDQ7016005.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEDYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            8777..9685
                     /gene="melR"
                     /locus_tag="QBH27_003743"
     CDS             8777..9685
                     /gene="melR"
                     /locus_tag="QBH27_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDQ7016006.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAILNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            9884..12151
                     /gene="adiA"
                     /locus_tag="QBH27_003744"
     CDS             9884..12151
                     /gene="adiA"
                     /locus_tag="QBH27_003744"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDQ7016007.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3128 a         3232 c         2993 g         2940 t
ORIGIN      
        1 ccgcttgggc acgtatcaat cactttcacc acagtggtga cgtcgacttc atccggcatg
       61 atccacggct ttcgtttgag attaaataac ttgccgttgc cacgtacgca attgccagaa
      121 tgctgacata tcgcagtgtt aaaatagaca tcgatatttt cgccggtata acagcgataa
      181 cccccgtcca gtagcgcctg atccatgacc ttccctctct catcagtatg gttttcacta
      241 taagcatagc cccggaatct tcatacagca ctggcagttt ccgttgccag agtctcttcc
      301 cctatatagc caatcattct tactcccttt gaattacccg cctcatcaga gataatgctt
      361 aagaaatttg tcacacaagg aagctgatga gacattcatt gccctaccgt atgttacgca
      421 aacgtccgat gaaattgagt accacagtga tcttaatggt cagcgcggta ctgttctcgg
      481 tgctattggt ggtgcatctg atttacttct cgcaaatcag tgatatgacg cgagacgggc
      541 tagctaacaa ggcactggca gtagcgcgta ccctcgccga ctcgccggaa atccgtcagg
      601 gcttgcagaa aaaagcgcag gagagtggaa tccaggccat cgcggaagcc gtgcgcaaac
      661 gcaacgatct gctgtttatt gtcgttaccg atatgcaaag tcttcgctac tcgcatcctg
      721 aagcccagcg tattggtcag ccatttaaag gtgatgacat ccttaaagcg ctgaatggcg
      781 aagaaaatgt cgctatcaat cgcggttttc tggcgcaggc tttacgcgta tttaccccca
      841 tctacgatga aaatcataaa caaattggcg tagtggcgat cggccttgag ttaagccgcg
      901 tgacccaaca gatcaatgac agtcgctgga gcattatctg gtcggtacta tttggcatgc
      961 tggtcggact gatcggcaca tgcattctgg ttaaagtact gaaaaaaatc cttttcggcc
     1021 tggaacccta cgaaatctcc acactgtttg agcaacgcca ggccatgttg cagtccatca
     1081 aagaaggcgt cgttgccgtg gacgatcgcg gcgaggtcac cctgatcaac gatgccgcac
     1141 aagaattgct gaattaccgt aagtcgcagg acgatgagaa actgtcgacg ctaagccact
     1201 cgtggtcaca agtggtagat gtctcggaag tgttacgcga cggaacccca cgccgcgacg
     1261 aagagattac gattaaagac cgactattac tgatcaacac cgttccggtg cgcagtaatg
     1321 gcgttatcat cggtgccatt tcaaccttca gggacaaaac tgaagtacgt aaactgatgc
     1381 agcgactcga cggtctggtc aactatgctg acgcacttcg tgaacgatcc cacgaattta
     1441 tgaataaatt gcatgtgatt ctcggattat tgcatctgaa gagttataag cagttggaag
     1501 attacattct caaaacagcc aataactatc aggaagagat tggctctctg ctgggtaaga
     1561 tcaaatctcc ggttatcgct ggttttttaa tcagcaagat taaccgcgcg accgatttag
     1621 gccatacgct gattttaaac agtgaaagcc agctgccaga cagcggtagt gaggatcagg
     1681 tcgcgacgct gattaccacg ttgggaaatc tgatagaaaa cgcgctggag gcattaggac
     1741 cggaacccgg tggcgaaatt agcgtaacat tgcactaccg tcacggctgg ctgcactgcg
     1801 aagttaacga tgatggaccg gggatcgcac ccgacaaaat cgatcacatt tttgacaaag
     1861 gtgtctcgac aaaaggaagc gagcgaggcg tcggtttagc acttgtcaaa caacaggtag
     1921 aaaatctcgg cggcagcatc gccgtggaat cggaacccgg gattttcaca caattttttg
     1981 tccagatacc ctgggacggg gagaggtcga acagatgatc aatgtattaa ttatcgatga
     2041 cgacgcaatg gtcgcggagc tgaatcgccg atacgtggca caaatcccgg gctttcaatg
     2101 ctgtggaacg gcttcgacgc tggagaaagc caaagagatt atcttcaata gcgatacgcc
     2161 tatcgacctg atattgctag atatctatat gcaaaaagag aacgggctcg atttactgcc
     2221 tgtcctgcat aacgcgcgtt gcaaaagtga tgtgattgtc atctcctccg ctgccgatgc
     2281 agcaaccatc aaagattcgc tgcattacgg tgttgtggat tacctgatca aacccttcca
     2341 ggcttcccgc tttgaagagg cgctcaccgg ctggcggcaa aagaaaatgg cgctggaaaa
     2401 acatcagtat tatgaccagg ccgagcttga tcagctaatc cacggcagca gctccaacga
     2461 gcaggatcct cgtcgcttgc caaaaggctt aacgccgcag acgttacgca cgctgtgcca
     2521 gtggattgac gcgcatcagg actatgagtt ttcaaccgac gagctggcta acgaggttaa
     2581 catctcgcgt gtttcctgcc gtaaatacct tatctggctg gtcaactgcc acatcttgtt
     2641 caccagtatc cattatggcg tcacggggcg tccggtttac cgttaccgta ttcaggcaga
     2701 acactactca ctactgaaac aatattgcca ataacggatc gctggttatc tgtaagtaat
     2761 aattattacc gtaatgcaaa atacccctgg cacaagttac atcgttcatc agtaaaactt
     2821 gtgccagatc aaatataatt atccctccat catcgctaaa aattaatatc tcttcagatg
     2881 aacggtgttt ttaatttcaa aacgctaaca aaagttaatt aactattatg tcacccacat
     2941 tatgtgtatt tttacccaca aatgggtaga tcagattaat ctataaacct aataatacct
     3001 gccctgagaa caaaaaacag accgataaat atcaataaga taacagcaaa caaaacatta
     3061 acatctgcgc tgtacaaact ataaacccat cgccagagag tctttctctc tgaaaaagcc
     3121 gcttatcaca gtgcataaat ttgccgctgc tttaatcagc caatattcac tgtgaggtat
     3181 ttgctcaagc cggtaacgac caaacggata tttagtcagg ctctgaaaac agttcataca
     3241 aaacagaacg tgactgtgat ctattcagca aaaatttaaa taggattatc gcgagggttc
     3301 acacatgtta tttactatcc aacttatcat aatactgata tgtctgtttt atggtgccag
     3361 aaagggtggt atcgcgctgg gtttattagg cggtatcggt ctggtcattc tggtcttcgt
     3421 cttccacctt cagccaggta aaccaccggt tgatgtcatg ctggttatca ttgcggtggt
     3481 ggcggcatcg gcgaccttgc aagcttcggg cggtcttgat gtcatgctgc aaattgccga
     3541 gaagctgctg cgtcgcaacc cgaaatatgt ctcaattgtc gcgccgtttg tgacctgtac
     3601 gctgaccatt ctttgcggta cgggtcatgt ggtttacacc attctgccga tcatctacga
     3661 cgttgctatt aagaacaaca tccgtccgga acgtccgatg gcggcaagtt ctatcggtgc
     3721 acagatgggg attatcgcca gtccggtgtc ggttgcggtc gtatctctgg ttgcgatgct
     3781 gggtaatgtc acctttgatg gtcgccatct tgagttcctc gacctgctgg caatcaccat
     3841 tccatcgacg ttaatcggta tcctggcgat cggtatcttc agctggttcc gcggtaaaga
     3901 tctggataaa gacgaagagt tccagaaatt catctccgta ccggaaaacc gtgagtatgt
     3961 ttacggtgat accgcgacgc tgctggataa aaaactgccg aaaagcaact ggctggcaat
     4021 gtggatcttc ctcggggcaa tcgctgtagt cgcccttctt ggtgctgatt cggacctgcg
     4081 tccatccttc ggcggcaaac cgctgtcgat ggtactggtt attcagatgt ttatgctgct
     4141 gaccggggcg ctgattatta tcctgaccaa aaccaatccc gcgtctatct caaaaaacga
     4201 agtcttccgt tccggtatga tcgccatcgt ggcggtgtac ggtatcgcat ggatggctga
     4261 aaccatgttc ggtgcgcata tgtctgaaat tcagggcgta ctgggtgaaa tggtgaaaga
     4321 gtatccgtgg gcctatgcca ttgttctgct gctggtttcc aagtttgtaa actctcaggc
     4381 tgcggcgctg gcggcgattg ttccggtcgc gctggcgatc ggcgttgatc cggcatacat
     4441 cgtggcttca gcaccggctt gctacggtta ttacatcctg ccgacttatc cgagcgatct
     4501 ggcagcgatt cagtttgacc gttccggcac cacccacatc ggtcgcttcg tcatcaacca
     4561 cagctttatt ctgccggggt tgattggtgt gagcgtatcg tgcgtcttcg gctggatctt
     4621 cgccgcgatg tacgggttct tataaatgca ctttgcgtgc cgcccgtaac tacgcggcac
     4681 gccattttcg aataacaaat acagagttac aggctggaag ctatgtcaaa caaacccttt
     4741 atctaccagg cacctttccc gatggggaaa gacaataccg aatattatct actcacttcc
     4801 gattacgtta gcgttgccga cttcgacggc gaaaccatcc tgaaagtgga accagaagcc
     4861 ctgaccctgc tggcgcagca agcctttcac gatgcttctt tcatgctccg cccggcacac
     4921 cagaaacagg ttgcggctat tcttcacgat ccagaagcca gcgaaaacga caagtacgtg
     4981 gcgctgcaat tcttaagaaa ctccgaaatc gccgccaaag gcgtgctgcc gacctgccag
     5041 gataccggca ccgcgatcat cgtcggtaaa aaaggtcagc gcgtgtggac cggtggcggt
     5101 gatgaagaag cactgtcgaa aggcgtctat aacacctata tcgaagataa cctgcgctat
     5161 tcacagaacg cgccgctgga catgtataaa gaggtcaaca ccggcacgaa cctgcctgcg
     5221 caaatcgacc tgtacgcggt agatggcgat gagtacaaat tcctctgtgt cgctaaaggc
     5281 ggcggctctg ccaacaaaac gtatctctac caggaaacca aagccctgct gacgcccggc
     5341 aaactgaaaa acttcctcgt cgagaaaatg cgtaccctcg gtactgcagc ctgcccgccg
     5401 taccatatcg cgtttgtgat tggcggtacg tctgcggaaa ccaacctgaa aaccgtcaag
     5461 ttagcaagcg ctcactatta cgatgaactg ccgacggaag ggaacgaaca tggtcaggcg
     5521 ttccgcgata tccagctgga acaggaactg ctggaagagg cacagaaact cggtcttggc
     5581 gcgcagtttg gcggtaaata cttcgcgcac gacatccgcg ttatccgtct gccacgtcac
     5641 ggcgcatcct gcccggtagg catgggagtc tcctgctccg ctgaccgtaa cattaaagcg
     5701 aaaatcaacc gcgaaggtat ctggatcgaa aaactggaac acaacccagg ccagtacatt
     5761 ccacaagaac tgcgtcaggc aggtgaaggc gaagcagtga aagtcgacct taaccgcccg
     5821 atgaaagaga tccttgccca gctttcgcaa tacccggtat ccacccgctt gtcgctcacc
     5881 ggcaccatta tcgtgggtcg cgatattgca cacgccaagc tgaaagagct gattgacgcc
     5941 ggtaaagaac tgccgcagta catcaaagat cacccgatct actacgcggg tccggcgaaa
     6001 acgcctgccg gttatccatc aggttcactt ggcccaacca ccgcaggtcg tatggactcc
     6061 tacgtggatc tgctgcaatc ccacggcggc agcatgatca tgctggcgaa aggtaaccgc
     6121 agtcagcagg ttaccgacgc gtgccataaa cacggcggct tctacctcgg tagtatcggc
     6181 ggtccggcgg cagtactggc gcagcagagc atcaagcatc tggaatgcgt cgcttatccg
     6241 gagctgggta tggaagctat ctggaaaatc gaagtagaag atttcccggc gtttatcctg
     6301 gtcgatgaca aaggtaacga cttcttccag cagatcgtca acaaacagtg cgcgaactgc
     6361 actaagtaac ctcttcggcc cagcgcctgg cagcatgctg ccaggtgatc cccctgggcc
     6421 acctcttttg cgattgtaat ttcacgcttg ctggtgaata gtcagtattt tccctgactt
     6481 gcgaactcac catgacccgc acactcaagc cgttaattct taacactggc gcactggcgc
     6541 taacgttaat cctgatttat accggcattt cggcccatga caaactcacc tggctgatgg
     6601 aagtgacacc ggtgattatt gtcgtgccgc tactgcttgc caccgctaaa cgttatccgt
     6661 taacgccgct gctttatacg ctcattttct ttcacgccat catcctgatg gtcggcgggc
     6721 aatacaccta cgcgaaagtc cccgttggtt ttgaggtgca ggaatggctg gggttgagcc
     6781 gtaatccgta tgacaaactg ggacattttt tccaggggct ggtgcctgca ctggtggcac
     6841 gagagatcct tgtacgcggg atgtacgttc gcggacgtaa gatggtggcg tttctggtct
     6901 gctgcgtggc gctggcgata agcgcgatgt atgaattaat cgagtggttg gcagcgctgg
     6961 caatgggcca gggagctgat gattttctcg gcacccaggg cgaccagtgg gacacgcaat
     7021 ccgatatgtt ctgcgcgctg cttggcgcat taacgacggt gatattcctc gctcgctttc
     7081 attgccgcca gctacggcgc tttggcttaa tcaccagagc gccggaagcg attacccctt
     7141 aacggtgcaa ccagcctggc agccagtcgc cgtgggcggc aatcaggtcg tcaacaagag
     7201 catatatttc gtcaatgccc agcacggcgg cagtatgcgg gtccatcatc gcggcgtggt
     7261 aaacacggtc gcgattttcc gtaagaatag cttctgtcag cagcgtctgt acgttgatgt
     7321 tggtttgcat cagggcggcc agatgcgaag gtagcgtacc ggctttggtc ggctgaatgc
     7381 cattagcatc aaccagacag gccacttcca cgcaacatcc ttgtggcagg ttatcaatca
     7441 aaccatcgtt acggacattg ccataaatca cactcggctc gccagtccag atagcgttca
     7501 tgattgtgct ggcatattcc cgtgacggtt taatatcaat ccgggaggcg tttttataat
     7561 cctccagctc tttatgccag ttcgccagct gctcgacgca gcgtttcggg tactcatcca
     7621 gcggtacttt ataacgctca atcaaatcct cacgacctgg cttaataaac cacggtgtgt
     7681 actcagcaaa atgttctgac gattctgtga cgaagtagcc cagctttttg aacatttcgt
     7741 aacgcacaat attctggcag cgagtattac catgaatatt tggcttcggt gcctgccctg
     7801 cttcataagc cgccagcaat tcaggataga gattcacgta gctaccgtct gcggttttac
     7861 gctccagttc caggtaaaac gccatatggt tgatacctgc gcaacggtaa cgcagcgtag
     7921 ccggatcgat attgaggtca cgcgccagct cttccgccgt tccctgcacc gaatggcaca
     7981 gccctacctg cttgatatgc ggatagcgtg catacatcgc ccaggtattc atcgccattg
     8041 ggttaacgta gttgagcatg gtggcatcgg ggcagacttc cgtcatgtcc tcgcaaatct
     8101 gccacaggtg cggaatggta cgtagcgcac gcataatgcc gcccggcccc agcgtatcgg
     8161 caatggtttg ttccagacca tgccgcttac agacctcgaa atcagtcacc gtgcaaggtt
     8221 cataaccgcc aatctgaaat gccaccacca caaaatcagc atcctgtaag gcttctttct
     8281 gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga atccatcaac ttgcgcacca
     8341 caatgtgtga ctcttccagg cgggtgggat cgatatccat cagggcaata tgcgccgttt
     8401 tcagcgcctc acgatggaac acatcaccaa gaatattttt aacgaaaatc gtcgaaccag
     8461 cgccgataaa tgtaatttta ggtgcagaca tcatgcagat ctcctggctt gcttgaatga
     8521 cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt ccaggttgag cattcccata
     8581 aactcatatc ctaagtggca catcaataga tctgagttta tgggaataaa ttacctgaaa
     8641 agctgaataa tcgaccccaa tcttctcatc cgctctcctc gcacctccag ggagaggaat
     8701 aaaaccgtgc gcctattcgc agtcatcctc cctcactcct gccataattc tgatattcca
     8761 ggaaagagag tcatccatga atacagatac gtttatgtgc agcagcgacg aaaagcagac
     8821 ccgtagccca ctgtcgctgt attcagaata ccagcggatg gaaattgagt ttcgcgcacc
     8881 gcatatcatg cccaccagcc actggcatgg tcaggtcgaa gtgaatgtgc ctttcgatgg
     8941 cgatgtggaa tacctgatca acaatgaaaa agtgaatatc aatcagggtc atatcacgct
     9001 gttctgggcc tgtacaccgc accaactaac agataccgga acctgtcaga gcatggcgat
     9061 tcttaatctg ccgatgcatc tgtttctctc ctggccgctg gataaagacc tgattaacca
     9121 cgttactcac ggcatggtga tcaaatcact ggcgacacag caacttagcc cgtttgaagt
     9181 gcgccgctgg cagcaggaat taaacagtcc gaacgagcaa attcgccagc tcgccattga
     9241 tgaaattggc ctgatgctca agcgatttag cctctctggc tgggaaccga ttctggtcaa
     9301 taaaacctcg cgcacacaca aaaacagtgt ctcgcgccat gcgcaatttt atgtcagcca
     9361 gatgctgggc tttattgccg aaaactatga tcaggcgctg accatcaacg atgtggctga
     9421 gcacgtcaaa cttaacgcca actatgcaat ggggatattc cagcgggtca tgcaattgac
     9481 gatgaaacag tacataaccg cgatgcgcat caaccacgtt cgcgcattac tgagcgatac
     9541 cgataaaagt attctcgata ttgccctgac ggcaggcttt cgttcgagta gccgttttta
     9601 cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa taccgcaaac ttagccaaca
     9661 gcgccgccag acgtttcccg gctaaagacg atatcagtat cagccaaaaa aatagtttag
     9721 ccgtcgataa taagcagtga aaataattta aaataatcac ataacatatt gtttataaaa
     9781 ataaatattt caaaaatgtt tgtttttcac gcgctttaca gcccgaaaag gccggaagat
     9841 acttgcccgc aacgaagatt ccttcataac cgggtaagca atgatgaaag tattaattgt
     9901 tgaaagcgag tttctccatc aagacacctg ggtcggtaac gccgttgagc gtctggcaga
     9961 tgctttaagc cagcaaaatg ttaccgtgat taaatccacc tcctttgatg atggttttgc
    10021 cattctctct tcaaacgaag ccattgactg cctgatgttc agctatcaaa tggaacatcc
    10081 ggacgaacat caaaacgtca gacaattgat cggtaagctt catgagcgcc aacaaaacgt
    10141 gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc gcaatggatc gcgacctgct
    10201 ggagcttgtc gatgaattcg cctggattct ggaagatacc gccgacttta tcgccggacg
    10261 cgccgttgcc gcgatgaccc gctaccgcca gcagctattg ccgccactgt tcagcgcgct
    10321 gatgaaatat agcgacatcc atgaatattc ctgggcagcg ccaggccacc agggcggcgt
    10381 tggttttacc aaaacacccg ccggacgttt ctaccatgac tactatggtg aaaatctgtt
    10441 ccgcaccgac atgggcatcg aacgcacttc cctcggttct ttgcttgacc atactggcgc
    10501 atttggcgaa agcgaaaaat atgccgcacg cgtatttggt gccgatcgct cctggtcggt
    10561 agtcgtcggt acttccggct ctaaccgcac catcatgcag gcttgcatga ccgataacga
    10621 tgtcgtggtc gttgaccgta actgccataa atccatcgaa caaggcttga tgctgacagg
    10681 tgcgaaaccg gtctacatgg tgccaagccg caaccgctac ggcattatcg ggccaatcta
    10741 tccgcaggaa atgcaacctg aaaccttgca gaagaaaatc aatgaaagcc cgctgactaa
    10801 agacaaagcc gggcaaaaac cgtcttactg cgtggtgacc aactgtacct atgacggcgt
    10861 gtgttataac gctaaagaag cgcaggatct gctggaaaaa acctccgatc gtctgcactt
    10921 tgacgaagcc tggtacggct atgcacgttt caacccgatc tatgccgatc actatgccat
    10981 gcgcggcgaa cctggcgatc acaacggtcc taccgttttc gccacccact ccacccacaa
    11041 actgctgaat gcgctgtcac aggcctctta tattcatgta cgtgaaggtc gtggggcgat
    11101 taacttctcc cgcttcaacc aggcctacat gatgcatgcc accacctccc cgctgtatgc
    11161 catctgcgca tccaacgacg tggcggtgtc gatgatggac ggcaacagcg gcctgtcact
    11221 gacccaggaa gtgatcgacg aagcggttga tttccgtcag gcgatggcgc ggctatataa
    11281 agagttcacc gctgacggta gctggttctt caaaccgtgg aacaaagaag tcgtcaccga
    11341 cccacaaacc ggcaaaacct atgactttgc tgacgcacca accaaactgc tgaccaccgt
    11401 tcaggactgc tgggtcatgc atccgggcga aagctggcac ggcttcaaag atattccgga
    11461 taactggagt atgctcgacc cgattaaagt cagcatcctt gctccgggaa tgggtgaaga
    11521 tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc acagcctggc ttggtcgcca
    11581 cggcattgtg cctacccgta ccactgactt ccagattatg ttcctgttct ctatgggcgt
    11641 gacccgtggg aaatggggaa ctctggttaa caccctttgc tccttcaaac gccactatga
    11701 cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt gaacaatatc ctgacactta
    11761 cgcgaacatg gggattcacg atctgggtga caccatgttt gcctggctga aagaaaacaa
    11821 ccctggcgca cggttgaatg aagcctattc cggcctaccg gtggcggaaa tcaccccgcg
    11881 tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg gtatccattg aaaatctgcc
    11941 aggacgtatc gcggcaaact cagttatccc gtatccgcca ggaatcccga tgctgctgtc
    12001 tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt tatttacgct cgctgcaatc
    12061 ctgggatcat catttccctg gatttgaaca cgaaacagaa gggacagaaa ttattgacgg
    12121 tatttaccac gttatgtgtg tgaaagcgta accactattc cgctgaaggc gtaattgtta
    12181 aataacatta cgccgcctgg cttgaggcct tttaagtatg gcaacgtttt cataaaaatt
    12241 gctgcaaaca aaaaatgtca tgctttttgc gcggtcccac cccgcgcttt ttt
//
LOCUS       DAOFSV010000132        12239 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.132, whole genome shotgun sequence.
ACCESSION   DAOFSV010000132 DAOFSV010000000
VERSION     DAOFSV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.132"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            237..2084
                     /gene="ldtD"
                     /locus_tag="QBH27_003745"
     CDS             237..2084
                     /gene="ldtD"
                     /locus_tag="QBH27_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ7016008.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2265..2813
                     /locus_tag="QBH27_003746"
     CDS             2265..2813
                     /locus_tag="QBH27_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDQ7016009.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2840..3487
                     /gene="gloC"
                     /locus_tag="QBH27_003747"
     CDS             2840..3487
                     /gene="gloC"
                     /locus_tag="QBH27_003747"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDQ7016010.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFNEQARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3537..4727)
                     /gene="aspC"
                     /locus_tag="QBH27_003748"
     CDS             complement(3537..4727)
                     /gene="aspC"
                     /locus_tag="QBH27_003748"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDQ7016011.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4912..6000)
                     /gene="ompF"
                     /locus_tag="QBH27_003749"
     CDS             complement(4912..6000)
                     /gene="ompF"
                     /locus_tag="QBH27_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDQ7016012.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGEDSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESPLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVDGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6603..8003)
                     /gene="asnS"
                     /locus_tag="QBH27_003750"
     CDS             complement(6603..8003)
                     /gene="asnS"
                     /locus_tag="QBH27_003750"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDQ7016013.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPNTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8172..9374)
                     /gene="pncB"
                     /locus_tag="QBH27_003751"
     CDS             complement(8172..9374)
                     /gene="pncB"
                     /locus_tag="QBH27_003751"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDQ7016014.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPLEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9640..>12239
                     /gene="pepN"
                     /locus_tag="QBH27_003752"
     CDS             9640..>12239
                     /gene="pepN"
                     /locus_tag="QBH27_003752"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDQ7016015.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKIT"
BASE COUNT         3118 a         3039 c         3112 g         2970 t
ORIGIN      
        1 ttttcttttt ccgaaaactc cgtttctgca ctaaaaaagt ggcacattac ggcgcgcttt
       61 tctttaaact tctttacatt aggttatgta aaaacgtatc ggcgtttata tactgaagat
      121 aagcctgatg agtaacaggc ttgctcgtca tactttcgtg agtattggcg ttgtacaggc
      181 aagtcgtaaa ataacagcct ggctattcag agtatgataa aaacaggggg caagggatgt
      241 tgcttaatat gatgtgtggt cgtcggctgt cggcaatcag tttgtgcctg gccgtaacat
      301 tcgctccact gttcaatgcg caggccgatg agcctgaagt aatccctggc gacagcccgg
      361 tggctgtcag tgaacagggc gaggcactgc cgcaggcgca agccacggca ataatggcgg
      421 ggatccagcc attgcctgaa ggtgcggctg aaaaagcccg cacgcaaatc gaatctcaat
      481 tacccgcagg ttataagccg gtttatctta accagcttca attgttgtat gccgcacgcg
      541 atatgcaacc catgtgggaa aaccgtgatg ctgttaaagc cttccagcaa cagctggcag
      601 aggtggcgat tgccggtttc cagccgcagt ttaataaatg ggtagagtta ctgaccgatc
      661 ctggtgttaa cgggatggca cgcgacgtgg tgctctctga tgcgatgatg ggctatctcc
      721 atttcattgc aaatattccg gtcaaaggca ctcgctggct atatagcagt aaaccttatg
      781 cgcttgcaac gccgccgctt tcggtgatta accaatggca gctggcgctg gataaaggtc
      841 aattgccgac gtttgttgca ggactggcac cgcagcatcc gcaatatgcg gcgatgcatg
      901 aatcgttact ggccttactc agtgacacca aaccgtggcc tcaactgacc ggtaaagcaa
      961 cgttgcgccc aggacagtgg agtaacgacg taccggcgtt gcgcgaaata ttgcaacgta
     1021 caggcatgtt ggacggggga ccgaaaatta cgctacctgg cgatgacacg ccaactgacg
     1081 cggtagtcag cccatcggct gttactgttg aaacagcaga aactaagctg atggacaaac
     1141 aaactacgtc tcgtagtaaa cctgcgcctg ccgttcgcgc cgcctacgat aatgaactgg
     1201 tggaagccgt taaacgtttt caggcatggc aaggattggg agcagatggt gctattggcc
     1261 cggcaacgcg tgactggtta aacgtaacgc ccgcccagcg tgctggcgtg ttggcactca
     1321 acatccagcg attgcgcttg ctgccaacag agctttctac cgggatcatg gttaacattc
     1381 cagcctattc gctggtctac tatcagaacg gcaatcaggt gctggattcg cgagtcattg
     1441 tcggtcgccc cgatcgcaaa acgccgatga tgagcagtgc acttaacaac gtagtggtaa
     1501 acccgccgtg gaacgtacca ccaactctgg cacgcaaaga tattctgccg aaagtgcgca
     1561 acgatccggg atatctcgaa agccatggct atacggtgat gcgcggctgg aacagcagag
     1621 aagcgattga cccatggcag gttgactggt ctacaatcac ggcctcgaat ttaccgttcc
     1681 gtttccagca ggctccaggc ccacggaact cgctggggcg ctataaattc aatatgccga
     1741 gttcagaggc catttatttg catgacacgc cgaaccacaa tctgttcaaa cgtgatacac
     1801 gcgcattgag ctcaggctgt gtacgagtga ataaagcttc cgatctggcg aatatgctgt
     1861 tgcaggatgc aggatggaat gacaaacgta tttctgatgc gctgaagcag ggcgatacgc
     1921 gttacgtcaa tattcggcag tcgattccgg tgaatctcta ctatctgacg gcctttgttg
     1981 gtgctgatgg tcgtacccag tatcgtacag atatttacaa ttatgatctg cctgcgcgat
     2041 ccagctcgca aattgtatcg aaagcggaac aattaatcag gtaaatgaag cagttcgggg
     2101 aaatcagttg tcgtaataat tagcatgatc gggggcgatt ctctgcagcc cccgtcactg
     2161 ctggggttga gtcatcttga cgtcttcttt acgggcggtt aaggtgcctc ttgtgcgcca
     2221 gaagtgcata taaacgataa cattgacctg tagacttgat tatcatggac aaattcgacg
     2281 ctaatcgccg caaactgctg gcgcttggtg gcgttgcact cggtgccgcc atcctgccga
     2341 ccccggcgtt tgcaacactc tctaccccac gcccgcgcat tttgacactc aataatcttc
     2401 ataccggaga gtcaatcaaa gcggagtttt tcgatggcag aggctatatt caggaagaat
     2461 tggcaaaact taaccatttt ttccgcgatt accgcgcgaa caaaataaag tccatcgacc
     2521 caggattatt cgaccagttg tatcgcctgc aagggttgtt aggcacccgc aaaccggtgc
     2581 aactcatttc cggttatcgt tccattgata ccaacaatga actacgcgcc cgcagccgtg
     2641 gagtagcgaa gaaaagctat cacaccaaag gccaggcgat ggatttccac attgaaggta
     2701 tcgcgttaag caatattcgc aaagccgcgt tatctatgcg cgcaggtggt gtaggatatt
     2761 acccacgtag taactttgtg catattgata ccgggccagc acggcactgg tagtaatcgc
     2821 ttaacgaaac aggggcagta tgaactatcg tattattccg gtcaccgcat tctcccagaa
     2881 ctgttcattg atctggtgtg aacaaacccg tctggccgca ctggtcgatc ctggcggcga
     2941 tgcggaaaaa atcaaacagg aagttgatgc cagcggcctg acactgatgc agatcctgct
     3001 gactcatggt catctggacc acgttggcgc agcggcggaa ctggcgcaac attacggcgt
     3061 gccggttttt ggcccggaaa aagaagatga gttctggctg caaggcttgc ctgcgcaaag
     3121 tcgtatgttt ggtctggaag agtgccagcc gctgacgcca gatcgttggc tgaacgaagg
     3181 cgataccatc agcataggga atgtgacttt acaggtgtta cattgccccg ggcatacgcc
     3241 gggtcatgtg gtgtttttta atgagcaggc aaggctgctc atttctggcg atgttatttt
     3301 caaaggcgga gtagggcgca gtgatttccc gcgtggcgat cacaatcaac tgatttcttc
     3361 aatcaaagat aaattgctgc cactggggga tgacgtgaca tttattccgg gtcacggacc
     3421 attatccact ctcggttatg agcgcctgca taatcccttc ttgcaagacg aaatgcctgt
     3481 ctggtaaagg cgcataaaaa agcccgcttt caagcgggct tcatcggttt taacgcttac
     3541 agcactgcca caatcgcttc gcacagcggt gccatgttat ctggtgtcat cccggccacg
     3601 tttacgcgac cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt
     3661 tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg
     3721 cgatttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc
     3781 tggcgcatat cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg
     3841 gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcacg aatcgccgct
     3901 ttcatttggc tgaatgcacg ttcaacagtt tcactgtcag cagcaaccag agtacaagcg
     3961 ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctcc
     4021 ttatgcagag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc ccgagcaaaa
     4081 ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caacggagag ttgtgccagt
     4141 gtctgccatt gttccagcgt agggtcgatg ccggttgggt tatggcagca gccatggaac
     4201 agcactacgt cgccagcctg ggcttcgttc aggctgttaa tcagtgcatc gaagtcaaga
     4261 gtgtgatttt ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag
     4321 acgctcttat ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt
     4381 tttgccagga aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg
     4441 cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag
     4501 cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc
     4561 agatactgtt cagccttttt cacgctggtc agtaccgggg ttttacccgt ctcatcttta
     4621 tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc
     4681 aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat
     4741 tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta
     4801 ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa
     4861 aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg
     4921 gtaaacgata cccacagcaa cggtatcgtc ggaacctacg cccaacttgt tgtcagaatc
     4981 gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc
     5041 gcccacttca aagtagttca ccagatcaac atcaccgata ccgtctacgt ctttcgcttt
     5101 agatttagtg taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa
     5161 cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttgt tagtgatcgg
     5221 cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt
     5281 cagaccagta gcccactgtt cagctttttt gcctttgcca aggggagact cttcctgcag
     5341 gttggtacgg tcagcggcac cataagcacc aacgatacca aagccttcgt attcgtagct
     5401 gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc
     5461 caggtactga acagcaaagt tcagaccatc aaccagacca aagaagttgg agttacgata
     5521 ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag tatcaccacc
     5581 aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc
     5641 gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt
     5701 accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc
     5761 ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt
     5821 catgtcgcca ttgccaccgt aactgtcttc accgttaccc ttggagaaat aatgcagacc
     5881 gacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt
     5941 tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat
     6001 tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat aagttctgtc
     6061 aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcaggcatct
     6121 ttccattcaa actaacgttt cgctaccgtg aaagtgctac aaagataaag atttggtttc
     6181 aaaaagaaaa aatgtgtgac aaaaagtaaa aattaaggaa ctttgtgaac accgtcatat
     6241 ttccatagag gcgtgatgat atttacagca atttttatct atttatatga tttccttata
     6301 tttaaattaa ctaaacggaa attttgtttc tgatggaaac tttatcgacc gggcacaaaa
     6361 tcttctttca gacatccaga atgccaaaaa atcgtataaa aattgtgcta tcagctaaga
     6421 aaaaactaat ccgcaataag aatgcagatt attttttgtg cgtaaatgtt gcagtgtaat
     6481 attccgtaac aggatgatcg ttatggcagg gttgcaaata gattgcttga cttaacaaac
     6541 agataacttg acagaaagga taaaaaaaga gccagcgggc gctggctctt gaagacacta
     6601 aattagaagc tggcgttacg cggagtacgt gggaacggaa taacatcacg tacgttttgc
     6661 acgccagtta cgtaagcaat caggcgttca aaaccaagac cgaaacctga atgcggaaca
     6721 gtaccgtagc gacgcagatc gcgataccac cagtaatctt ctttgttcag gcccatttca
     6781 agcatacgct cgtccagcac gtccagacgt tcttcacgct gggagccacc aatgatctca
     6841 ccgatgcccg gagccagaac gtccatagcc gcaacggttt taccgtcttc gttaaggcgc
     6901 atatagaacg ctttaatatc tttcggatag tttttaacca ctactggtgc tttaaagtgt
     6961 tcttccgcca gataacgctc atgctcagaa gagagatcga cgccccagta aaccgggttt
     7021 tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgcg
     7081 aaatcggcct caatgaagcg ttccagacgt gaaacggcat ctttatctac gcgttcagcg
     7141 aagaatttca tgtcgtcagc gcgttcttcg agaaccgctt tgaagacata tttcagcatg
     7201 gcttcagcca gacctgcgat atcgttcagg ttagcaaacg ccacttccgg ctccagcatc
     7261 cagaattccg ccaggtgacg gctggtgttg gagttttcag cacggaaagt cgggccgaag
     7321 gtataaattt tggacaatgc gcaagcgtag gtttcgccgt tcaactggcc agatacggtc
     7381 aggaaagact ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg atcgttacgc
     7441 ggcaggtttt ccagatccag cgtagaaaca cggaacattt cgcctgcacc ttcggtatca
     7501 gatgcggtaa tcagtggcgt tgaaacccag aagaatccct gctcgttaaa gaagcgatgc
     7561 agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga
     7621 cgcaggtgag cgacttcacg cagatactca atgctgtggc gttttgccgc catcgggtaa
     7681 gtgtttggat cttcaaccca accagcaact tcaaccttgc tggcctgaat ttcaaattgt
     7741 tgcccctggc ccggcgacgc cacgacttta cccgttacaa tgaccgagca gccggtggtc
     7801 agacgcagga cgtcttcatt gtaattgggc agagaattat tgatgacagc ctgtacagga
     7861 tcaaagcagg aaccgtcata aacggcgagg aaggagatgc cagcttttga atctcggcgg
     7921 gtacgtaccc atccgcgcac ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg
     7981 tcggctacag gcacaacgct cataatattc tctctgttaa tagtcggaaa aaataaacac
     8041 ttgtccaccc gtaaatgggg ggatacctat gttacctggc atctgcaatc agacaagcag
     8101 aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa aaaaagggag ccgattagct
     8161 ccctgatgat attaactggc ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc
     8221 cgaacaaacg ctttatcgtg gcagatcgtt ttgccagggc tgtcagaaag tttcgccacc
     8281 ggtttaccgt tacactctac caacttaatg acaatattca gaggttttac ctgggggata
     8341 tcgcaggtca ggcgagtccc aataccaaaa cttaattgca cgcgggaaga gaagtggcgg
     8401 tatagctcaa ccgctttgcg taaatccaga ttgtcagaga aaaccagcgt tttactctgt
     8461 ggatcaattc ccagcttttc ataatgtgca atggcttttt caccccattc aagcgggtcg
     8521 ccagagtcat ggcgcaggcc ctggtaacga ctggcaaatt ccacgccgaa atctcgcagg
     8581 aaagcatcca tggtgatgca gtcggttaac gcaatgccaa gttggtcggg atactcttcc
     8641 agccaggcag caagtgcagc tcgctggctg ttggctagat ccgggctgat ttgctgatgc
     8701 gcctggaacc attcgtgtgc ctgtgttccc attggcgtga gggaaagccg acgcgccaga
     8761 tcgtagttgc tggtgcccac gaaccaggat tcctgttgca gacgtttcac gatggtttct
     8821 tgtacttcgc gagaaaaacg gcggcgggtg ccaaaatcca tcagatggaa gcgcgacata
     8881 tcaagaccgg cggttaatgc cgagaagtcg actaatttgt tttccagcgt gtcgagggct
     8941 tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca tttcactgat aaccgccagc
     9001 aaaggaactt cccagaggat gacttcacgc cacgggccgc ttaaacgaat atccagcttg
     9061 ccattatcgt tggacacggt gacttgttcc gggttaaagc ggaactcgcg tagccagtta
     9121 agatagtcgg ccttaaagaa aggcagggca gagagccact gatattcatc atcctgcagg
     9181 cgcaggtgct gcatcgcctg aacctgttca cgaatagcat cggcataaat acccagcaga
     9241 tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat cgtaatagtg atgaaacacg
     9301 gcttgctgca tatgcaactt ataagcatct gtatccagca acgagtgcag aacaggagaa
     9361 gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag gagcgtttag tacaataaac
     9421 atcttcagga actgctggag tataccttgt ttcgcaattt attgaacccc gatcacacca
     9481 tatgccacct ttctggtcga tggcataacg cggtgtatgt tataaaaatg tagcaataaa
     9541 ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg atttacgcaa caggaataga
     9601 ctgaacacca gactctataa aagatgctaa aggttattta tgactcaaca gccacaagcc
     9661 aaataccgtc acgattatcg tgcgccggat taccagatta ctgatattga cttgaccttt
     9721 gacctggacg cgcaaaagac ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca
     9781 tcagatgttc cgcttcgtct caacggcgaa gacctcaaac tggtttctgt tcatattaat
     9841 gatgagccgt ggaccgcctg gaaagaagaa gagggcgcac tggtcatcag taatttgccg
     9901 gagcgtttta cgcttaagat cgttaatgaa attagcccgg cggcgaatac tgcgctggaa
     9961 gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg tttccgccat
    10021 attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc
    10081 gataaaacca aatatccctt cctgctttcc aacggtaacc gcgttgcgca aggtgaactg
    10141 gaaaatggac gtcattgggt acagtggcag gatccattcc cgaaaccgtg ttacctgttt
    10201 gcgctggtgg ctggtgattt tgatgtactg cgcgatacct tcactacccg ctctggtcgt
    10261 gaagtggcgc tggagttgta tgtcgatcgt ggcaaccttg atcgtgcgcc gtgggcaatg
    10321 acctcgctga aaaactccat gaaatgggat gaagaacgct tcggcctgga gtatgacctc
    10381 gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga gaataagggg
    10441 ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac cgacaaagat
    10501 tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac cggcaaccgt
    10561 gtcacctgcc gcgactggtt ccagctcagt ctgaaagaag gtttaaccgt cttccgcgat
    10621 caggaattca gctctgacct tggttcccgc gcggtaaacc gtatcaataa tgtgcgcacc
    10681 atgcgcggat tgcagtttgc agaagacgcc agcccgatgg cgcacccaat tcgcccggat
    10741 atggtcattg aaatgaacaa cttctacacc ctgaccgttt acgagaaggg cgcggaagtg
    10801 attcgtatga tccacactct gcttggcgaa gaaaatttcc agaaagggat gcagctctat
    10861 ttcgagcgtc atgatggtag cgcagcgacc tgtgacgatt ttgtgcaggc gatggaagat
    10921 gcgtcgaatg tcgatctctc ccatttccgc cgttggtaca gccagtccgg cacgccggtt
    10981 gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac catcagccag
    11041 cgcacgccag ctacgccgga tcaggcagaa aaacaaccgc tgcatattcc gtttgccatc
    11101 gaactgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca tccggtgaat
    11161 tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt ctacttccag
    11221 ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata taagtggagc
    11281 gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctctcg ctgggacgcg
    11341 gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag
    11401 ccgttgtctc tgccggtgca tgtggctgac gctttccgcg cggtgctgct cgatgagaag
    11461 attgatccgg cgctggcggc agaaatcctg acgctgcctt ccgtcaatga aatggcagaa
    11521 ctgttcgata tcatcgatcc gattgctatt gccgaagtac gcgaagcact cactcgtact
    11581 ctggcgactg aactggcaga tgagctgctg gctatttaca acgcgaatta ccagagcgag
    11641 taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg cctgcgcttc
    11701 ctcgcttttg gtgaaacgca tctggctgat gtgctggtga gcaaacagtt ccacgaagcg
    11761 aacaatatga ccgatgcgct ggcggcgctt tctgcggcgg ttgctgcaca actgccatgc
    11821 cgtgacgcgc tgatgcagga gtacgacgac aagtggcatc aggatggtct ggtgatggat
    11881 aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac ggtacgtggt
    11941 ctgttgcagc atcgctcatt taccatgagc aacccgaacc gcattcgttc attgattggc
    12001 gcatttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg ttatcagttc
    12061 ctggtggaaa tgcttaccga cctgaacagc cgaaatccac aagtggcttc gcgcctgatt
    12121 gaaccgttga ttcgcctgaa acgttacgat gccaaacgtc aggagaaaat gcgcgcggcg
    12181 ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tgtacgagaa gatcaccaa
//
LOCUS       DAOFSV010000133        12223 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.133, whole genome shotgun sequence.
ACCESSION   DAOFSV010000133 DAOFSV010000000
VERSION     DAOFSV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.133"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..420)
                     /locus_tag="QBH27_003753"
     CDS             complement(<1..420)
                     /locus_tag="QBH27_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDQ7016016.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFL"
     gene            complement(467..1186)
                     /gene="yehT"
                     /locus_tag="QBH27_003754"
     CDS             complement(467..1186)
                     /gene="yehT"
                     /locus_tag="QBH27_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDQ7016017.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1183..2868)
                     /gene="btsS"
                     /locus_tag="QBH27_003755"
     CDS             complement(1183..2868)
                     /gene="btsS"
                     /locus_tag="QBH27_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDQ7016018.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3090..3821
                     /gene="mlrA"
                     /locus_tag="QBH27_003756"
     CDS             3090..3821
                     /gene="mlrA"
                     /locus_tag="QBH27_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDQ7016019.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQTLLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            3881..3988
                     /locus_tag="QBH27_003757"
     CDS             3881..3988
                     /locus_tag="QBH27_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDQ7016020.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3969..4700)
                     /gene="yehW"
                     /locus_tag="QBH27_003758"
     CDS             complement(3969..4700)
                     /gene="yehW"
                     /locus_tag="QBH27_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDQ7016021.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(4705..5631)
                     /gene="yehX"
                     /locus_tag="QBH27_003759"
     CDS             complement(4705..5631)
                     /gene="yehX"
                     /locus_tag="QBH27_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDQ7016022.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(5624..6781)
                     /gene="yehY"
                     /locus_tag="QBH27_003760"
     CDS             complement(5624..6781)
                     /gene="yehY"
                     /locus_tag="QBH27_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDQ7016023.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGVQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(6788..7705)
                     /gene="osmF"
                     /locus_tag="QBH27_003761"
     CDS             complement(6788..7705)
                     /gene="osmF"
                     /locus_tag="QBH27_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDQ7016024.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNVQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7895..10192)
                     /gene="bglX"
                     /locus_tag="QBH27_003762"
     CDS             complement(7895..10192)
                     /gene="bglX"
                     /locus_tag="QBH27_003762"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDQ7016025.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            10388..12103
                     /gene="dld"
                     /locus_tag="QBH27_003763"
     CDS             10388..12103
                     /gene="dld"
                     /locus_tag="QBH27_003763"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDQ7016026.1"
                     /translation="MSSMTTTDNKAFLNELSRLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
BASE COUNT         2988 a         3414 c         3019 g         2802 t
ORIGIN      
        1 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcacgca tcatcgccgt
       61 aatttccggc atcgaaacgc ggaactgctg ttcggtgagg attgccagaa tgtcatcggt
      121 tttaagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc
      181 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
      241 gaatgaagac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
      301 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
      361 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
      421 gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
      481 cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
      541 tcaactcggc ctggccgtta tcttccagac ggatctcctg taaatgcgcg aggttaacca
      601 gatactgacg atggcagcgc agtaatggtg tacgactttc cagggtacgt aatgtcagtt
      661 cggtaaagcc ctctttccct tcgtgactgg taacgtagac accgctcatc cgactgctga
      721 caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa
      781 atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac
      841 gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
      901 aggcatgttc ttcaaaggct ttaatggcgt attcgtcaaa cgcggtgaga aaaacaatat
      961 acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct
     1021 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
     1081 cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acgcgcaggt
     1141 tctcccgtgc aagcggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
     1201 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttatcccata
     1261 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
     1321 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
     1381 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
     1441 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
     1501 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
     1561 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
     1621 gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
     1681 gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatggggat tcacctgggc
     1741 gtgaagcagt ttgatctctg actgggtgag catcgctttt tgccgctcat attgcccggc
     1801 gaggatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt tgattgaact
     1861 gaataaacgg tttttggctt catacaattt gatggtaccc atcactcgct gattttcacc
     1921 acgcaacgga atgaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacggta
     1981 aggcacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtata
     2041 agtcgaagaa atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag
     2101 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
     2161 tgccactttc atgctgttca cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc
     2221 cgccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
     2281 atcgagcaat atacgcataa acaacgccgc gccgacggta ttggtgacca tcattggcgc
     2341 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
     2401 caacatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
     2461 tttatcagtg cgcccgcgac ggatcaggat gctgtgaacc aggccgccga gcaatccttc
     2521 aacgatggtc gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
     2581 cccgccggtc agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
     2641 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
     2701 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
     2761 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa
     2821 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
     2881 cacttccctt taaaatgcgt aacatacatt gcttgcgttt aactttcttt gaactcttgc
     2941 agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc
     3001 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
     3061 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
     3121 tgctttgtga tattaaccct gtcacgttac gcgcgtggca gaggcgttac ggattgctga
     3181 aaccgcaacg gacagacggc ggccatcggc tgttcaacga tgccgatatt gaccggatcc
     3241 gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagtaaaa atgctgctca
     3301 gtaatgaaaa tgttgatgtg cagaacggct ggcgcgatca gcaagaaaca ttactgactt
     3361 acctgcaaag cggcaatctg catagcctgc gaacgtggat caaagagcgc ggtcaggatt
     3421 accccgccca gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc
     3481 aacaaccgac tctccagacg ctgctggcga tcctcgacgg cgtactgatc aactacatct
     3541 ctatttgcct ggcttcggcg cgcaaaaaac agggtaaaga tgcactggtg gttggctgga
     3601 atattcagga taccactcgt ctgtggctgg agggctggat tgccagtcaa caaggatggc
     3661 gcattgatgt cctcgcccac tcgctcaatc aactacgccc tgaactgttc gaaggccgta
     3721 cattgctggt gtggtgcggt gataatcgaa cctccgccca acagcagcaa ctcaccagtt
     3781 ggcaagaaca aggccatgat attttcccac tcggcattta atgattcgtt aacaaatgcg
     3841 ctttactgta caatcctttc gttaacataa ggagtgcatt atgcgcatag ctaaaattgg
     3901 ggtcatcgcc ctgttcctgt ttatggcgtt aggcggaatt ggtggcgtca tgctcgcagg
     3961 ttataccttt attttgcgtg ctggctgaac gcctgcacca gcctttcaaa caggcggtct
     4021 gcgatgatcg ccgccagtgc caccagtaac gctccctgga tcacatacgc ggtattaaat
     4081 ccgctaagtc cgatgatgat gggcgtaccc agcgtgctgg cccctaccgt tgaggcgatc
     4141 gtcgccgtac caatgttgat aatcaccgaa gttcgcacgc cagccagaat caccggagcc
     4201 gccagcggta gctcgacctt acgcagtcgc tgaccacgac tcattcccat acctttcgca
     4261 acttctgtca cgctggcatc aatcgctccc agcccggcaa gtgtcgcctg caggacgggc
     4321 agcacgccgt aaaggatcaa ggcgataatc gctggtttca gaccaaagcc gatcaccgga
     4381 acggcaatcg ccagcaccgc gacgggcgga aaagtctgcc caacggcggc aatagtttcc
     4441 accagtgggc gaaattccgc accccacggg cgagtgacag caattccggc accagtacca
     4501 atgatcaccg caaacaaact cgaaattccc accagccaga aatgagccag tgccagagct
     4561 gcaaaacttt cttgctgata aacgggtcgt ggcagttgtg ggaacaaggc agcaaacagc
     4621 ggctggctat aaggtagcca gaaaatcagt gccacaaaca aagcaatgag ccagaacagc
     4681 ggatcgcgca acatcttcat acgcttacgc ctcctccagc agatcctgaa aatgcagcgt
     4741 gccgcaaggc tggccctgcg tgttcaccac cggcagcacc tcgcatcccc gcgcgacaaa
     4801 cagagaaagc gcatcgcgta gcgtcatcct ttcttccagt gcctcacctt cagcccgttc
     4861 ttcacggcgc acgtaatctg ccacgctacg taacgaaagc aggcgtacac ccagctcact
     4921 acgaccaaaa aactggcgga caaaatcatt cgccggatga gtcagcatcg ccagaggatt
     4981 gccctgctgc acgacttcac cgtgatccat caataccaga tgttctgcca gccgtagcgc
     5041 ctcatcaata tcatgagtga ccagcacaat ggtacgcccc tgcaaacggt gaatgcgcgt
     5101 catctcttgt tgcaacgcgc cgcgcgttac cgggtccagc gcgccaaaag gttcatccat
     5161 cagtaagact tgcggatcgg cagccagtgc gcgcgccact cccacacgtt gctgctgacc
     5221 accggaaagc tgatgcggat aacgctcacg caaatttggc tccagcccca gtagcgccat
     5281 taattcgtcg atacgatcgt cgatccgcgc ccgtgaccat ttttgcaatt gcggcacggt
     5341 ggcgatgttt tgtgccacgc tccagtgggg aaacaggcca atagattgaa tggcatagcc
     5401 catccggcgg cgcaactcca gtactggcag cgagcgaatt tcttctccgg caaagcggat
     5461 caccccgctg tcatgctcca ccaggcggtt aatcattttc agagtggtgg atttaccgga
     5521 gcctgatgta ccaatcagca ccgaaaaact cccttcctga aaattgagat tgagatcgtt
     5581 aacggctttt tgtgcgccga acagtttgct gacatggcta aattcaatca ttacgtttca
     5641 ccttcagcag tgcgataagc aaatcgaaca gcgcgtcggt aagcaccgcc agaacaatta
     5701 ccgggatcac ccccagcaac actaaatcaa tggcactgct tagcagcccc tggaaaacca
     5761 gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac cgccatgcca acggtttgca
     5821 ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc cagcggtaac tgaacatgca
     5881 ggaaacgctg caccccgctc atccccatcg ctctggcgct ctccagcaca tcgcgcggga
     5941 tctggttcaa gccgactact acgccgcgca ccagcggcag caaggcatag agcaccagcg
     6001 caataagtgc gggtgtcatt ccggttcctg ctatgccgag cgttcccagc cacggaaagg
     6061 ccgtaaccag cgcggcaagc ggcgcaatca gcaggccaaa gagcgctacc gaaggcacgg
     6121 tctgaatgac attcagcaga gaaaaaatcg ccccctgccg cgcggtggaa aagtagcacc
     6181 agatgcccaa cggcacacca atcactaacg caggcagcac cgcaccaaac agcaacgtca
     6241 gatgttgtgc cagagcgtcg tcaaacacat cctgacggtt ggcgtattct ttcattaacg
     6301 agagatcgtt aagcgtgccg gagtacagca accacagcgg aataatggca atctgcatat
     6361 gcaacaacca gcgccacagc ggatgcgtgg agattcggcg gatggcatcg ctacaggcca
     6421 gcagtgccag cgccgcagcc agccagaaac cactgccgag gctggtacgt gccagcgcac
     6481 tgccattttg cgccagttgt gtcgccgcct ttccagctcc ccacaccagc aatacgaaga
     6541 cgaattgcgc cagaatgagt gcaaaaatgc tgcctttttt agcgggaata aaacaggctg
     6601 tcagccaggc gcaaccaacg cccaccagca tccagagagt ttgcggccac agctgccaga
     6661 gatgacgccc ctcacccgaa actaaacgat taggcgcgta actgataaac ggcagcgccg
     6721 ctgcgattgc cgtcagcaac agcagcagcg ccagaacagg attaatacgt agataagtca
     6781 cgggaaatta cttcgtccac cctttttgtt tcaggtagtc ggcagccact tttttggcat
     6841 ccagtccttc cacagcaatg ctggcattca gttgctgcaa tgttttttca tcgaggctgg
     6901 cgaagactgg ctgtagccac tgtgccattt gtggatactc tttcagcacc gactcacgca
     6961 ccactggtgc aggcgcgtag ataggttgca cgccttgcgg atcgcttaag gtttgcagcc
     7021 ccagcgccgc gaccgggccg tcagtgccgt aagccattgc ggcattaacg ccagaggttt
     7081 gctgggcagc ggctttgatc gttaccgccg tgtcgccacc agccagtgac agcaactgat
     7141 cctgaccgag cttaaagcca taggcttttt caaacgcggg taaggcatcg gcgcgttcaa
     7201 taaactctgc cgaggctgcc agtttgaagg tgccgccctc ttgcagataa cgactcaggt
     7261 cggcaagcga agtgagcttg tttttctctg ccacatcctg acgcacggcg atggtccagg
     7321 tgttatttgc aggtgcgggc gtcagccaga ttaacttgtt ttgctctgca tcgagttttt
     7381 tgactttctc gtaaccttgc tgcacgtttt tccacgctgc atcgttttca tctttaaaga
     7441 agaacgcccc attgccggta tattccggat agatatccag ctcgccagaa gtgatcgcgc
     7501 cgcgcaccac aggagtcgtt ccaagttgca ctttatttac cgtaggaacg ccgtgacttt
     7561 ccagcacctg caaaatgata ttgccgagca gcgcgccttc cgtatcgatt tttgaaccaa
     7621 ccttaacggg ggaagctgcc tgtagcggca ggctcacggc tgccaacaaa accagtgaac
     7681 ctgcccagac ctttgagagt ggcatgatgc tttcctcatt cttttactgt tgttttcagc
     7741 gaattaagag aaaagcatag ttgataatga cagggttagc ctgtctgggt ggattcagtt
     7801 gcagaatcag ataaatactt aaaaggcata ttttgcccgg cgtttcttgc cggatgcggc
     7861 gtaaacgcct tatccggcct acaaacatcg aaaattacaa caactcaaac tcggctttct
     7921 taacgcgtgc ggaatcagtg ccgataaaga cattgaactt gccaggctcg gcgtcatatt
     7981 tcatctgttg attccagaac ttcagcgcct caatatcaat cgggaagctg acggtctgag
     8041 tttcgcccgg tttcagagtg attttctcaa agcctttcag ctgcttcacc gggcgactca
     8101 tggaagccgt cacatcctgc aagtacatct gtactaccgt ggccccttcg cgtttaccgg
     8161 tgttcgtcac ctgcacgctg gccgtcactt tgccgtcacg cttcatggtc ggcgcagaaa
     8221 gtttcacatc agagacggtg aaagtggtgt agctcagccc atagccgaac ggataaagcg
     8281 ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg gcattatacg
     8341 gacgcccggt attcagatgg ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg
     8401 acatcggcag cttgccggac gggttataat cgccaaacaa tacatcggca attgcattac
     8461 cgccttcagt ccccgcaaac caggtttcca gaatcgcatc agcctgctga tcttctttca
     8521 ccagcgccag cggacgccca ttcatcagca ccagcaccag cggtttaccg gtggctttca
     8581 gcgccgcaat caagtcacgt tggctttgcg gaatagtgat atcggtacga ctggaagctt
     8641 cgtgcgccat cccctgagct tcaccgacca cagccaccac cacatcagat tgtttcgcgg
     8701 tttgcaccgc ttcatcaatc atctcttgcg gcgaacgcgg atcgactttg accgcttctt
     8761 catactgatt caggaaatcg ataatgcctt tgtcactggt aacgttcgcc cctttggcat
     8821 acagcacttt accgttttca ccgacggcgt ttttaatccc ggttagcaca gtaacagatt
     8881 gatcggcgac acctgctgcc gaccagcttc ccatcacgtc acgcttgctg tctgccagcg
     8941 ggccaaccac cgcaatggtt gcagattttt tcagcggcag cgtttcgaga cggtttttca
     9001 gcaacaccag gctttcgcgc gccacttcac gcgcttcttt gcggtgcagg cggctttcgg
     9061 cattggtatc caccgggtca gactctttcg gccccaaatg gctgtatgga tcgttaaaca
     9121 atcccatatc atatttaacg ttcagtacat ggcgggcagc atcgtccagc tcttccatcg
     9181 tcactttgcc ggatttgatc aacccaggca gatacttcga gtaatactcg tcgctcatgc
     9241 tcatgttgat tccggacttc agcgccacgc gtaccgcatc ctctgggtct gccgccgtgc
     9301 catgtttaat cagttctttg attgcgccgt gatcggaaac ggtgatgcct ttaaagcccc
     9361 actggtcgcg cagaacatct ttcagcagcc aggagtcgga ggtggctggc gtgccgttca
     9421 gcgagttcag cgccaccatc accgcaccgc tgcctgcatc cagccccgct ttgtacggcg
     9481 gcatatagtc gttaaacagg cgctgcggac tcatatcgac ggtgttgtac tctttaccgc
     9541 cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag tagcgatctg
     9601 ccgggctttt accctgcatc gcttccacca tggttttacc cattattgag gtgagatacg
     9661 tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca
     9721 tcggtgccca ggtcatattc aggccatcat ctgccgcttc ataagcagag acacgcccga
     9781 ctgttttcac cgcatcgagg ttaaaagacg aggccagacc gagactaatc gggaacaccg
     9841 tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg cggcttaatt
     9901 ccatcacctg atcctgcatg gcgcggatat cctgacgggt tacggtgttg aaaatcgccc
     9961 caacctgacc gtctttgatc atctcgcgga tcgcctcttt cgggttatcc gggccgacgc
    10021 tgattaagcg cagctgacca attttctcat caactgtcat tttcttaagc agttcggtga
    10081 cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca tccgccagtg
    10141 ctggctgcag ggccagactc accgcgattc ctactgaaca tagccatttc atatggattt
    10201 ttcttccttt gttgcccgac gtggcagcga aaatggtgca aaaaccgtag tttgccataa
    10261 gcatgatgga gagagaaaaa gaatgctcag tttattgtct gaattttcaa aatattcact
    10321 cgctgaattg tcatacaagg cgctattcta gtttgtgata ttttttcgcc accacaagga
    10381 gtggaaaatg tcttccatga caacaactga taataaagcc tttttgaatg aactttcccg
    10441 tctggtcggt cattcacacc tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg
    10501 cttccgttct ggtcagggcg acgcgctggc tgtcgttttc cctggctcac tactggaatt
    10561 gtggcgggtg ctgaaagcct gcgtcaccgc tgacaaaatt attctgatgc aggcggccaa
    10621 tacaggcctg accgaaggat cgacgccaaa cggtaacgat tatgatcgcg atatcgtgat
    10681 catcagcacc ctgcgtctcg acaagctgca cgtactcggt aagggcgaac aggtgctggc
    10741 ctatccgggc accacgctct attcactgga aaaagccctc aaaccgctgg gacgcgaacc
    10801 gcactcagtg attggatcat cgtgtatagg cgcatcggtc atcggcggta tttgtaacaa
    10861 ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg
    10921 tataaatgaa gatggcaaac tgacgctggt gaaccatctg gggattgatc tgggcgaaac
    10981 gccggagcag atccttagca agctggatga cgatcgcatc aaagatgacg atgtgcgcca
    11041 cgatggtcgt cacgcccacg attatgacta tgtccaccgc gttcgtgata ttgaagccga
    11101 cacgcccgca cgttataacg ctgatccgga tcggttattt gaatcttctg gttgcgcagg
    11161 gaagctggcc gtctttgcag tacgtcttga taccttcgaa gcggaaaaaa atcagcaggt
    11221 gttttatatc ggcaccaacc agccggaagt gctgaccgaa atccgccgtc atattctggc
    11281 taatttcgaa aatctgccgg ttgccgggga atatatgcac cgggatatct acgatattgc
    11341 ggaaaaatac ggcaaagaca ccttcctgat gattgataag ttaggcaccg acaagatgcc
    11401 gttcttcttt aatctcaagg gacgcaccga tgcgatgctg gagaaagtga aattcttccg
    11461 tccgcatttt accgaccgtg caatgcaaaa attcggtcac ctgttcccca gccatttacc
    11521 gccgcgcatg aaaaactggc gcgataaata cgagcatcat ctgctgttaa aaatggcggg
    11581 cgatggcgtc ggtgaagcca aatcgtggct ggtggattat ttcaaacagg ccgagggcga
    11641 tttctttgtc tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc
    11701 gggcgcagca attcgttatc aggctgtgca ttccgatgaa gtcgaagaca ttctggcgct
    11761 ggatatcgct ctgcggcgta acgacaccga atggtatgag catttaccgc cggagatcga
    11821 cagccagctg gtgcacaagc tctattacgg ccattttatg tgctatgtct tccatcagga
    11881 ttacatcgtg aaaaaaggcg tggatgtgca tgcgttgaaa gaacagatgc tggaactgct
    11941 tcagcagcgt ggcgcgcaat accctgccga acataacgtc ggtcatttgt ataaagcacc
    12001 ggagacgttg cagaagtttt atcgcgagaa cgatccgacc aacagtatga atccggggat
    12061 cggtaaaacc agtaagcgga aaaactggca ggaagtggag taataattac ggatggcaga
    12121 gtatcgccat ccgtcattca tttaatcgtt ctgtgccgtc tgccccgccg ccgccatttg
    12181 ggcggctttt tgttttttat agctcaacgc tgctgccgga aca
//
LOCUS       DAOFSV010000134        12083 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.134, whole genome shotgun sequence.
ACCESSION   DAOFSV010000134 DAOFSV010000000
VERSION     DAOFSV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.134"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(105..1445)
                     /gene="lamB"
                     /locus_tag="QBH27_003764"
     CDS             complement(105..1445)
                     /gene="lamB"
                     /locus_tag="QBH27_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDQ7016027.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(1517..2632)
                     /gene="malK"
                     /locus_tag="QBH27_003765"
     CDS             complement(1517..2632)
                     /gene="malK"
                     /locus_tag="QBH27_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDQ7016028.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            2997..4187
                     /gene="malE"
                     /locus_tag="QBH27_003766"
     CDS             2997..4187
                     /gene="malE"
                     /locus_tag="QBH27_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDQ7016029.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFETDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            4341..5885
                     /gene="malF"
                     /locus_tag="QBH27_003767"
     CDS             4341..5885
                     /gene="malF"
                     /locus_tag="QBH27_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDQ7016030.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            5900..6790
                     /gene="malG"
                     /locus_tag="QBH27_003768"
     CDS             5900..6790
                     /gene="malG"
                     /locus_tag="QBH27_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDQ7016031.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(6884..7294)
                     /gene="psiE"
                     /locus_tag="QBH27_003769"
     CDS             complement(6884..7294)
                     /gene="psiE"
                     /locus_tag="QBH27_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDQ7016032.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            7509..7787
                     /locus_tag="QBH27_003770"
     CDS             7509..7787
                     /locus_tag="QBH27_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016033.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(7834..9930)
                     /locus_tag="QBH27_003771"
     CDS             complement(7834..9930)
                     /locus_tag="QBH27_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDQ7016034.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSIGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(9930..10667)
                     /locus_tag="QBH27_003772"
     CDS             complement(9930..10667)
                     /locus_tag="QBH27_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDQ7016035.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSDAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALMTRMAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(10664..11302)
                     /locus_tag="QBH27_003773"
     CDS             complement(10664..11302)
                     /locus_tag="QBH27_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDQ7016036.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(11417..11659)
                     /locus_tag="QBH27_003774"
     CDS             complement(11417..11659)
                     /locus_tag="QBH27_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016037.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         2849 a         3220 c         3093 g         2921 t
ORIGIN      
        1 tactttcggc agcggttgta ggcctgataa gacgcaacag cgtcgcatca ggcattgatt
       61 gccggatgcg gtgcaaacac cttatccggc ccagtttttt gctattacca ccagatttcc
      121 atctgagcac cgaaggtcca ctcgtcgctg tcgccacgac cgaagctacc gccattgaaa
      181 tcagccgaca cagccttgcc gtagtatggg ttggttgaag agctaccggt gtagtcataa
      241 ccccatttct catcccactt ggcgtaggtt gcgaagacac gaatagccgg gcgtgaccag
      301 atgctgtcgc cagcctgcca ttgttgtgcg agggtaattt tgtactgatt gttcttgtcg
      361 ccggtgcgct gggattcgac gttgtcgtag ccgatttcca tcacggtgct catgattggc
      421 gtccacttgt acatcgggcg aataccgacg gtccaccact tggtgccgtt gtcgttatcc
      481 cagttgatat cctggtacat acccacgtac atcatgtccc agttgtcgcc catggagatc
      541 gcaccgtggt cgaggatacg cagcatgtga ccgttgttat tgatattgta ggcaaatttt
      601 tcgttatcaa acgcgacgcc agaaccctgc gacagacctt taccctgcga ggtcatcgag
      661 tcagtagcgt actgaacaac aaacttgtta aagcccttca ggacactctg agtatgttca
      721 gcagtgaata accagccgtc tttcgatgcg ccatcaacca gacgatagtt atcacgcagg
      781 ttggcacgac cgtagtcgac acccagttct aatgtgccgc ccgggttgat ttccatctgc
      841 gctaaacgca catcgaaaac gtcgttcgcg gtttcgttgg tatagtcata aatattgttg
      901 ctggcgaaag aggaagaacc ccccgcttca gaggagcggg ttgctgccag agagagttta
      961 ccgaagccaa catcgatgtt ttccagaccg gcaccaggac cagaaatatc ccagtagtag
     1021 aagtcgatca tatgaacgtc atgacgttgg tagaagcgct tacctgccca gatggtggag
     1081 cctggcagcc attcgatcag gtttttaccc tgcacgtttg cttcacggaa agccggatcg
     1141 gtagcttccc agtcattctg ttgcgcgacg gaataggcca cgttagtgtc gaaatagaag
     1201 ctcttatcgc cctctttcca cacttcctga cccaatttta attcagcata agtttcacat
     1261 tcgttgccaa gacggtattt actttgagca ccggtagtct ggaaacactg ttgttcgccg
     1321 ccgctacctg tccagccaat accggaacgt gcatagccgt ggaaatcaac agccattgcc
     1381 tgagcagaca ttacgcccgc tgcgacggca accgccagag gaagtttgcg cagagtaatc
     1441 atcattctat ctcctgagtc attgcttttc ttttttcaca tcacctgtga caggctttgt
     1501 gtgttttgtg gggtgcttaa acgcccggct ccttatgcag tcgacgacat gcagtgccat
     1561 cctcacggaa cagatggcaa cgctctggcg gcaggccgat agcgaatgtg gcaccttctt
     1621 ctaccaacac cacgtcgttc tggcggtaca ccaggttttg acgaatggaa gggatctgga
     1681 tatggatttg cgtttcgttg ccgagttgct cgacgacctg aacttcaccc tcaaggatga
     1741 cgtcagcgat atcactcggc agtagatgct ccgggcgaat acccagcgac atattggctc
     1801 ccacctggac atcacggctt tcgaccggta gccagacttg ctgacgattc ggcatcggca
     1861 gctccacctg cacttgatcg attgcggtgg cggtcacttt tactggcagg aagttcatct
     1921 ttggcgaacc gataaatccg gcgacaaaac ggtctgccgg atagtgatac agctccagcg
     1981 gtttcccaac ctgcgccacg cgaccagcgt ccagcaccac gattttgtcg gccagcgtca
     2041 tcgcttcgac ctgatcgtgg gtgacgtaaa tcattgtgcg gcccaggcgt ttatgcagac
     2101 gggagatttc gatacgcatt tgcacacgca gtgcagcatc gaggttggag agcggttcat
     2161 cgagcaaaaa tacgcttggc tcggccacca gcgtacggcc aatcgccaca cgctgacgct
     2221 gaccaccgga gagcgctttc ggtttgcgat ccagcaaatg cgccagttgc agcacttccg
     2281 caacctggtt aactcgctgg ttaatcactt cttttttcgc gccagccagt ttcaggccaa
     2341 atgacatgtt ttctgctact gacaggtggg gatagagcgc gtaagactga aacaccatac
     2401 caacgccgcg ttctgcaggc ggagtgtcat tcatccgttt ctcaccgatg aacaggtcgc
     2461 cgctggtgat cgtctcaagc ccggcaatca tgcgcagtaa agtcgattta ccgcagccag
     2521 acggtccgac aaacaccacg aattcacctt catggatatc gagattgata tctttcgata
     2581 ccacgacctc gccccaggct ttcgttacat tttgcagctg tacgctcgcc atgcccttct
     2641 ccctttgtaa caacctgtca tcgacagcaa cattcatgat gggctgacta tgcgtcatca
     2701 ggagatggct taaatcctcc accccctggc ttttttatgg gggaggaggc gggaggatga
     2761 gaacgcggct tctgtgaact aaaccgaggt catgtaagga atttcgtgat gttgcttgca
     2821 aaaatcgtgg cgattttatg tgcgcatctc cacattaccg ccaattctgt aacagagatc
     2881 acacaaagca acgatggggc gtaggggcaa ggaggatgga aagaggttgc cgtataaaga
     2941 aactagagtc cgtttaggtg ttttcacgag cacttcacca acaaggacca tagattatga
     3001 aaataaaaac aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg
     3061 cctcggctct cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag
     3121 gctataacgg tctcgctgaa gtcggtaaga aattcgagac agataccgga attaaagtca
     3181 ccgttgagca tccggataaa ctggaagaga aattccctca ggttgcggca actggcgatg
     3241 gccctgacat tatcttctgg gcgcacgacc gctttggtgg ctatgctcaa tctggcctgt
     3301 tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg
     3361 ccgtacgtta caacggcaag ttgattgctt acccgatcgc tgtcgaagcg ttatcactga
     3421 tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg
     3481 ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccatact
     3541 tcacctggcc gctaattgcg gctgacgggg gttatgcgtt caagtacgaa aacggcaagt
     3601 acgacattaa agacgtaggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg
     3661 ttgatctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg
     3721 cctttaataa aggcgaaaca gcgatgacta tcaacggccc gtgggcatgg tccaacatcg
     3781 acaccagtaa agtgaattat ggtgtaacgg tactgccgac cttcaaaggt caaccatcta
     3841 aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg
     3901 cgaaagagtt cctcgaaaac tatctgctga ctgacgaagg tctggaagcg gtcaataaag
     3961 acaaaccgct gggtgcagta gcgctgaagt cctatcagga tgaacttgcg aaagatccac
     4021 gtattgccgc caccatggat aacgctcaga aaggtgaaat tatgccgaac atcccgcaga
     4081 tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga
     4141 ctgtggatgc tgcgctgaaa gatgcacaga ctcgtatcac caagtaatgc tgtgaaatgc
     4201 cggatgcggc gtgaacgcct tatccggcct acgaaattga aacgtatgta ggcctgataa
     4261 gacgcgtcag cgtcgcatca ggcagttgtt gtcggataag gtatgaaagc cttatccgtc
     4321 ctggaatgag gaagaacccc atggatgtca ttaaaaagaa acattggtgg caaagcgacg
     4381 cgctgaaatg gtcagtgtta ggtctgctcg gcctgctggt gggttacctt gttgttttaa
     4441 tgtacgcaca aggggaatac ctgttcgcca ttaccacgct gatattgagc tcagcggggc
     4501 tgtatatttt cgccaatcgt aaagcctacg cctggcgcta tgtttacccg ggaatggctg
     4561 gaatgggatt attcgtcctc ttccctctgg tctgcaccat cgccattgcc ttcaccaact
     4621 acagcagcac taaccagctg acttttgaac gtgcgcagga agtgttgcta gatcgctcct
     4681 ggcaagcagg caaaatctat aattttggtc tttatccggc gggcgatgag tggcaactgg
     4741 cgctcagcga cggcgaaacc ggcaaaaatt acctctccga cgcttttaaa tttggcggcg
     4801 agcaaaaact gcaactgaaa gaaacgaccg cccagcccga aggcgaacgc gcgaatctgc
     4861 gcgtaattac ccagaatcgt caggcactga gtgacattac cgccattctg ccggatggca
     4921 acaaagtgat gatgagctcc ctgcgccagt tttctggcac gcagccgctc tacacactcg
     4981 acggtgacgg cacgctgacc aataatcaga gtggcgtaaa atatcgtcca aacgaccaga
     5041 ttggttttta ccagtccatc actgccgacg gtaactgggg tgatgaaaag ctaagccccg
     5101 gttacaccgt gaccaccggc tggaaaaact ttacccgcgt ctttaccgac gaaggcattc
     5161 agaaaccgtt cctcgccatt ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt
     5221 taacggtggc ggtcggcatg gttctggcgt gtctggtgca gtgggaagcg ttgcgcggca
     5281 aagcggtcta tcgcgtcctg ctgattctgc cctacgcggt gccatcgttc atttcaatct
     5341 tgattttcaa agggttgttt aaccagagtt ttggtgaaat caacatgatg ttgagcgcgc
     5401 tgtttggcgt gaagcccgcc tggttcagcg atccgaccac cgcccgcacg atgctgatta
     5461 tcgtcaacac ctggctgggt tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag
     5521 cgattccgga cgatttgtat gaagcctcag caatggatgg cgcaggtcca ttccagaact
     5581 tctttaagat tacgctgccg ctgctgatta aaccgctgac gccgctgatg atcgccagct
     5641 tcgcctttaa ctttaacaac ttcgtgctga ttcaactgtt aaccaacggc ggcccggatc
     5701 gtcttggcac gaccacgcca gccggttata ccgacctgct tgttaactac acctaccgta
     5761 tcgcttttga aggcggcggg ggtcaggact tcggtctggc agcagcaatt gccacgctga
     5821 tcttcctgct ggtgggcgcg ctggcgatag tgaacctgaa agccacgcga atgaagtttg
     5881 attaagggag ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag cacgtttgtt
     5941 tattactcac ctgctactgc tactttttat cgcggcaatt atgttcccgc tgctgatggt
     6001 cgtcgccatc tcgctgcgtc aggggaactt cgcgaccggt agtctgatcc cggagcaaat
     6061 ctcctgggat cactggaaac tggcgttagg ttttagcgtg gaacaggctg atggtcgcat
     6121 tacgccaccg ccattcccgg tactgctgtg gctgtggaac tcggtaaagg tcgccgggat
     6181 ttccgcgatt ggcattgtgg cgctctccac cacctgcgcc tacgctttcg cccgtatgcg
     6241 ctttccaggc aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt tcccggcagt
     6301 actttcactg gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca ttccattcat
     6361 tggcctgaat actcacggcg gcgtaatttt cgcgtatctg ggcgggattg cgctgcatgt
     6421 ctggaccatc aaaggttatt tcgaaaccat cgacagttcg ctggaagaag cggcggcgct
     6481 ggatggtgcg acaccgtggc aggccttccg ccttgtcctg ttgccgctgt cagtaccgat
     6541 tctggcggtt gtgttcatcc tgtcgtttat cgctgccatt actgaagttc cggtcgcgtc
     6601 gctattactg cgtgacgtaa acagctacac cctggccgtg gggatgcagc aatacctcaa
     6661 cccgcaaaac tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg cattaccgat
     6721 caccatcgtc ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg caggtggtgt
     6781 gaaaggttaa agatgttgtt ctgccaatgt tatgccgctg caccctcaac ttacgttatc
     6841 ccaacttgtg actgttattc ggcgctccac ggagcgcctt tttttactcc cgcttcagcc
     6901 gtttcgaatt acacaaccac agggtgatca ccagtaacag gatcgctgcc gagtagatca
     6961 acacatccag tggcgattta tgatcgacga tgatcaagcg cacaatggca gtgatcccaa
     7021 tatagacaaa gtaacgtagc gggaagtgaa aaccggactg aaagtacttc acaatcagcg
     7081 cgataaattc gaaatagaga aagtaaacca ccagaccttc taccagctca tatttactgg
     7141 tttgttctgg cgcgaacagc acatcagcca gatgcaccgt ttctttgccg aggaagacaa
     7201 ccaaaatcag gccgaggcac aacaggccaa gattgagtac ggtctgcaaa atggtggaga
     7261 taaactccac gcgcggacga gagagtgacg tcatagcagt acctccttca cagtggcgag
     7321 cttcctgtat aacgcaaaaa tgtgacggag atctatattt tttgtgtatg ttttgttcag
     7381 ctcaattaag tgggcatctc ccatcaatgt ctcatctggc aaaacacaca gcccaaaaaa
     7441 atcacccatt caggtgattc ccagccacca gcaatgcgcg aaaaatcgac aatgttcctc
     7501 tggaacaaat gaaacgtaac ctgataaagg tggtaaaaat gaagccttat tttgctgctt
     7561 tgatgttatc agtctctgtt cttcctgctt atgctggtcc gctcggtact gctgataaag
     7621 ccgacctacc gcaaagcaat gtctccagcc caatgatggc ccagtcgctc aggcaaccag
     7681 acctgcaacc gatatcgacg gacaggaaga cagaatgttt tcgcctttac acaccagatc
     7741 gcaaaccggg ggtgaactgc gttcctgatg gctcgacagg ccattaagaa taaaggtgcc
     7801 agtgagtgcg aaacctggca cctttcccat gacttagcgg aaattgacgc tacggtcgct
     7861 ggtcatgtca tacaactgga acttacgccc aagttgctga ccaccgtcac gcgtcagcgg
     7921 cgtccagcca atcgccgcac ggctgcgtgt cgggccagac gagaagagat ccagcggtac
     7981 agagacatac acgcctttgg tgaagtcccc ttcgccgtac tcctctttcg aaacattcgt
     8041 gatcgtggca tagccgccaa ccaccacgcc gctgtcaaag cgtttagcga tctccagcgt
     8101 gccgccttta tccccagcca gatactgccc gacgctcgct ttcactaaca cgtcctgggc
     8161 gaaagatggc gtccagtacg cggtcagatg tccggttttc acgctgtaat cggtgaattt
     8221 catcatatct tttgcgctac gccagtcgcg ctgtttaacg tagttggcat ccagaccaaa
     8281 cgcccagttg ctatccagcg ggcgatacaa cacttctgcc cccacgccgc caaacatggt
     8341 ttcgagataa cctccgtaga cctgaccgta gaagccgttg cccagatgct ggaagtagtt
     8401 ggcttgcagg ttattcacat agacatcatt ctgcacatac tcgcgcacat gggtacgcac
     8461 gcgcggtaag tgcgagtcct gcggtggatt ggtgtagtta aacttgtcgt agttgttggc
     8521 aaggttagca aacaggctgc cagtggtcag cagatggtcc gtcagccaca aatccgccgt
     8581 tcccatcacg cccagctgat acatgtaaaa gttttccggg ccaccgatcg actggttcag
     8641 caccggatcg atatggaaat caaagcgtga tttgtcgata taccagccct gctcggtgga
     8701 cttcggaacc actggctcga cgcgtttttg cgccagcttc gtttcgtggc ccaacggctc
     8761 tccggcgaga tgatttttca ggctggcgac atcggtttcc gtcgtcacct gtggcatatt
     8821 aaggcgattt tccgtaatgc ggatcgtttt tatcccatcc ggcagatcgt tcatcacgat
     8881 ccgattagca cggatgatcc cttcgcgcgg atcacgatat ttcacctgct cgccggtaac
     8941 atacagcgta tcgcctttcg cctggatctg cggatcggca agtccggcat tgtatttcaa
     9001 cagcgttaac tgattcgcca ccaccgaatg ctgcaaaatg gcatcctgcg gctgcggttg
     9061 atattgcggg cgggcgttat cgttgtaaga cgggcgcaga tcgttaaagt tggtgcgcag
     9121 cgtgacgcca aacataaaag tgttgccacg ttcatagcta aggttaacgt cggcccaatc
     9181 ggtaacgcga taaatcgccc cgacgttaaa cttacttttt tgctccagct ttccggcaaa
     9241 atcctgctga taattattgc cttcatactc cagtttcaga cgcagcggtt gccagggcgt
     9301 ctggtattcc acgccgccaa acagtgaggc cgggccgtgg aacatctggc tgccgtcgat
     9361 agatcccgcc tgtttgtagc tgttatcgcg atagcaatat ttatcactgg ctgaacagag
     9421 cggatttttc acgttaccgc tggtgcccag atatccccag cccaggccga gtgtaaaatc
     9481 gaacggcccc caggctttgc tggcaacaag atattccgca tcaaacagcc ccgtaccgcc
     9541 gatatcccgc gcaccaaccg ccacttgcgg cagccaataa ctctcttccc acaaacgcag
     9601 tttgagatcg aaggctttat ctttatacgt ttgatcgcca gagaacgctt cgacgctgct
     9661 gtactgccgg gtgcgcacgt cggtgtagcg cagcgttgtt tccagccacg ggaagagttg
     9721 cactgaagct gagtaataac ggtactgatc gttatcgcga tagttcaggc tcagctcccc
     9781 ttcccgcgcc atgcgcgcgg tgggcgtttg taataatcct acgccaccga aatccgattg
     9841 cgacggacca atgggcgcag gatatgtttc gccgtagcaa gctgtgctaa tacccagcgc
     9901 cagtaagcta agcagatgtc tttttttcat tattggggta tccgctgcgt cagagtctga
     9961 agaatgtcgg cgttaagggc atcaggtgtc tcactccaga cggagtccgc gaggccaaca
    10021 taaataatgc tgccgggcat cggctctacg tgacgcttgt tccagtaagc caccggcgct
    10081 ttttgcgtgc gtccgtccgg gtaaaccacc cacgcgtagc tgcgatccgc accgctgagc
    10141 aggctctggt cagagagata gctcgccacg tcgcgacctg gagtgaatga ctgcttgcca
    10201 ggacgactga taagcccgaa caacgtgacc gtggacggcg gcggtccgac ccacagcgtg
    10261 tagttgccct gcaacggcgg gttaccgcgt tcggcaacgc ggacgatatc aggatcaaga
    10321 ttgatttttt gcctgcccgt cactttcaac gcctgaattt gctggcgtaa ggcgttaatt
    10381 gcggcggcat cgtcggcgct ggagtctgcg gccagttctg ccatccgtgt catcaacgcc
    10441 tgctgctgac gcaatgctgc cgccgtggcc agctcttcgc taatcaccgc accaggccac
    10501 cagctgtttg ccagccgcgg ttggcctacc agatcgatta aatgctctgc acccgtcagc
    10561 gttttggcat cactgctgcc attgctgaat accttgacgg ttcccgccgc gaagaccgat
    10621 gacgccccca cactcaaaat caatgcgaca atagtttgtt taatcattgt tttgccgcct
    10681 tgatgagggt ggttttcacc gggaagtaat ccgcgccgag atactgttcc gactggcgaa
    10741 tttgcccttc gctgtcgatc cagtagcggt tatgccagcg cgcctggtcg gtggagactt
    10801 cttcgtccag cacgcgaacc ggggtttcat cgctgccgac tttgacggta tcggtgccat
    10861 cccatttgaa gactgagcgt gcggtggcgt agcgtacctg ctggtactcg gtccagccca
    10921 tcgtgcgcgt ccaggttgca ccatcaacaa tttgtgcggg tttaatcagc gggtcggcgg
    10981 caaggttatt cacttcaatc aggttgtcgc cgccaagcaa agtcttcacc agacgaccat
    11041 gttgtgtgac aagggtggcc tgatcctgag tgacccattt ttgttgtccg tcttcagcga
    11101 aggccagcac cacaaataac tgcggcccgc cattaagctg catgtactgg ctggcgtagg
    11161 gcatattttg aatatcatcg tccgtcagct gtacgcctgg cgtaccgaac agactgtccc
    11221 acagtgaatt gcccagctct ttagtcgtgg ctgaacaggc ctgtaatagc aggcagataa
    11281 gaatgagtgc tggtcgcttc acgactcttc tccagagggg cagaataacc acaccggagt
    11341 gtggttatgg ttaatcccct tcatacttca agcttcagat gcgatgactc gaattatttt
    11401 ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc ccggtattag
    11461 aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt acggtgctga
    11521 cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgatcct gccgcttcgc
    11581 ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg gcaaaaatgc
    11641 catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat ttacccaaaa
    11701 catttcgggc gggatcgagt atacacaact aaagcatgcg aattacggcg cggggaatta
    11761 gtgatggagt tttaggacaa ttggaatgaa tatcaaatat aagctttgac ttatatataa
    11821 ataagattaa aaatcatgaa gttaaaataa aataaaaagt aattactctg cgtattttcc
    11881 gaaataaacc acgattaaaa atttatttta ggattaacct cacggtatga ttttcgttaa
    11941 atgacagaca agaggattta tctgataaaa caatgccgac ttatagtcgg cattgttttt
    12001 aaggctattt aatcaatcgt actatgtatt aattgccgaa tacgatgtac atatcggcat
    12061 cgacctgtag gcctgataag acg
//
LOCUS       DAOFSV010000135        12014 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.135, whole genome shotgun sequence.
ACCESSION   DAOFSV010000135 DAOFSV010000000
VERSION     DAOFSV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.135"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(48..794)
                     /gene="cutC"
                     /locus_tag="QBH27_003775"
     CDS             complement(48..794)
                     /gene="cutC"
                     /locus_tag="QBH27_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDQ7016038.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(808..1374)
                     /locus_tag="QBH27_003776"
     CDS             complement(808..1374)
                     /locus_tag="QBH27_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ7016039.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1590..3323
                     /gene="argS"
                     /locus_tag="QBH27_003777"
     CDS             1590..3323
                     /gene="argS"
                     /locus_tag="QBH27_003777"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDQ7016040.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3376..3768)
                     /gene="flhe"
                     /locus_tag="QBH27_003778"
     CDS             complement(3376..3768)
                     /gene="flhe"
                     /locus_tag="QBH27_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDQ7016041.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTMAFSGIPAAEPL
                     RFIWEVPGGGRLTPPLKIQRNEVIVNYR"
     gene            complement(3768..5846)
                     /gene="flhA"
                     /locus_tag="QBH27_003779"
     CDS             complement(3768..5846)
                     /gene="flhA"
                     /locus_tag="QBH27_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDQ7016042.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNSVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5839..6987)
                     /gene="flhB"
                     /locus_tag="QBH27_003780"
     CDS             complement(5839..6987)
                     /gene="flhB"
                     /locus_tag="QBH27_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDQ7016043.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRTFSAQTGAELLKAILKTILVGSVTG
                     FFLWYHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7176..7820)
                     /gene="cheZ"
                     /locus_tag="QBH27_003781"
     CDS             complement(7176..7820)
                     /gene="cheZ"
                     /locus_tag="QBH27_003781"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDQ7016044.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVIDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQGQVDDLLDSLGF"
     gene            complement(7831..8220)
                     /gene="cheY"
                     /locus_tag="QBH27_003782"
     CDS             complement(7831..8220)
                     /gene="cheY"
                     /locus_tag="QBH27_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDQ7016045.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8235..9284)
                     /gene="cheB"
                     /locus_tag="QBH27_003783"
     CDS             complement(8235..9284)
                     /gene="cheB"
                     /locus_tag="QBH27_003783"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDQ7016046.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9287..10147)
                     /gene="cheR"
                     /locus_tag="QBH27_003784"
     CDS             complement(9287..10147)
                     /gene="cheR"
                     /locus_tag="QBH27_003784"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDQ7016047.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10262..10392)
                     /locus_tag="QBH27_003785"
                     /pseudo
     CDS             complement(10262..10392)
                     /locus_tag="QBH27_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(10438..>12014)
                     /gene="tar"
                     /locus_tag="QBH27_003786"
     CDS             complement(10438..>12014)
                     /gene="tar"
                     /locus_tag="QBH27_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDQ7016048.1"
                     /translation="FSSLHHSQKSFVVSNQLREQQSELTSTWDLMLQTRINLSRSAVR
                     MMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYH
                     TALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRF
                     AQWQLAVIALVVVLILLAAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEM
                     GDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAA
                     SMEQLAATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADII
                     SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIED
                     SVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAVSEMD
                     RVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPA
                     QPRLRIAEQDPNWETF"
BASE COUNT         2865 a         3389 c         2984 g         2776 t
ORIGIN      
        1 tggtacgagc aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga
       61 tggcgttcaa tgattccttt catttcagca accgccgccc cgtctacgac ataacgcgaa
      121 tactcgtccg cgtgtgcatc tgatgacatg gacaatcctt gattacgata acgcatcggt
      181 gaggcttgcc acgctcccgc ggagctatgg acttccagca ctccggcatc gaggaagtgg
      241 tgcaggtttt ccgcacggac tcctgctccg gccataatga ttggagcatc acgatgggca
      301 ataagttcca taatttttga taaaccttgc agtgcgtctg atttttgccc tgatgtcagt
      361 acccgggcaa tgcccaattc cgtaagatta ttgagtgtat ttaaagggtt agcgcacata
      421 tcgaaggcgc gatgaaaagt cactgccagc ggaccggcag cagccattat tttttccatt
      481 cgtggcatat cgacattccc gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct
      541 aattcgcgga ctgtgcgcac atcttcaaga atggcggcaa actcaccgtc gctgtagcaa
      601 aaatcaccac cgcgtgggcg gataatcgga tgcactggga tcgtcacccg ctggcgcacg
      661 gatttcagta cccccaacga cggcgttagg cccccctctt ttggagctgc gcataattca
      721 actctgtcag cgccgttttg ctgcgccgtt agcgcgcatt ccatgctgta acagcaaatt
      781 tccagtaatg ccatttttgc tccttagtta cgccgactgc tcgctggcaa cgatctcttc
      841 aatggaccac ggatgaaact taatggttgt tttaccatcg gtgaccgcca gcgtcggatt
      901 cggtaatcgt tcatgctcac cttttggcga actggtttta actgagatcc ccggcaggct
      961 gagtccttca tcagaaagca atgccagcgc acgggcattc agcgtttctg gatcacccgg
     1021 tagaacgatt tcaatatgtt cccaaccttc gtgcgggtaa cgtttttccc cgggccacgg
     1081 tagctccaca atggaaaact gccagtgcgc aacctgtagc ggttcatgca atttaaacaa
     1141 acaaatcggt ctgccattga tcatattttc tgacaaaagc tcgccgcact gttcaaaccc
     1201 gcgacgccag cgttcagcgg tgacgttttg atggcaacgc aaagaaatgt gatcggcagt
     1261 cagcggagtg atatccaacc ccagacggcg ggaaagttca tctaatgcgt gggtaaatcg
     1321 cggtaaatcc gatgcaatat cctgcagctc gtcgatagat tgccagttcg ccataatcat
     1381 tcttcgtctt tcattaaaag cgttaattta ccctgttgcc ctgcgccaac caaccgctga
     1441 tttcacgccg cttctgatgc aatagtgaaa acggcaatac gccacgcgca cgttgctgac
     1501 gaaaacagcc atttgcagta tactcccgcc ctaatttctt taactggtgc gggtaatttt
     1561 tgctcgcttc atcaatgtaa ggtattccgg tgaatattca ggctcttctc tcagaaaaag
     1621 tccgtcaggc catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcagt
     1681 cagcaaaagt tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac
     1741 tgggtatggc accgcgacaa ttagcagagc aggtgctgac tcatctggat cttaacggta
     1801 tcgccagcaa agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat
     1861 tcctggctga acatgttcag caggcgctgg cgtccgatcg tctcggtgtt gctacgccag
     1921 aaaaacagac cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcatgtcg
     1981 gtcacctgcg ctctaccatt attggtgacg cagcagttcg tactctggag ttcctcggtc
     2041 acaaagtgat tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg
     2101 catggctgga aaagcagcag caggaaaacg ccggtgaaat ggagctggct gaccttgaag
     2161 gtttctaccg cgatgcgaaa aagcattacg acgaggatga agagtttgcc gagcgtgcac
     2221 gtaactacgt ggtaaaactg caaagcggtg acgaatattt ccgcgagatg tggcgcaaac
     2281 tggtcgacat caccatgacg cagaaccaga tcacctacga tcgtctgaac gtgacgttga
     2341 cccgcgatga cgtcatgggt gaaagtctct acaacccaat gctgccgggt atcgtggcgg
     2401 acctgaaagc caaaggtctg gcagtagaaa gtgaaggcgc gaccgttgta ttccttgatg
     2461 agttcaaaaa caaggaaggc gaaccgatgg gcgttatcat tcagaagaaa gatggcggct
     2521 atctctacac caccactgat atcgcctgcg cgaaatatcg ttatgaaaca ctgcatgccg
     2581 atcgcgtgct gtattacatc gattcccgtc agcatcaaca cctgatgcag gcatgggcga
     2641 tcgtccgtaa agcgggctat gtaccggaat ccgtaccgct ggaacaccac atgttcggca
     2701 tgatgctggg caaagatggt aaaccgttca aaacccgcgc tggcggtacg gtgaaactgg
     2761 ccgatctgct ggatgaagcc ctggagcgtg cacgtcgtct ggtggcagaa aagaacccgg
     2821 atatgccagc cgacgagctg gaaaaactgg ctaacgcggt tggtattggt gcggtgaaat
     2881 atgcggatct ctccaaaaac cgcaccacgg actacatctt cgactgggac aacatgctgg
     2941 cgtttgaggg taataccgcg ccatacatgc agtatgctta cacgcgtgta ttgtccgtgt
     3001 tccgtaaagc agaaattaac gaagagcaac tggctgcagc tccggtaatc atccgtgaag
     3061 atcgtgaagc gcaactggca gctcgcctgc tgcagtttga agaaaccctc actgtggttg
     3121 cccgtgaagg cacgccgcat gtgatgtgtg cttacctgta cgatctggcc ggtctgttct
     3181 ctggcttcta tgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg cgtaacagcc
     3241 gtctgaaact ggcacaactg acggcgaaga cgctgaagct gggtctggat acgctgggta
     3301 ttgagacagt agagcgtatg taatcgcgtt atacggcaac agccgatgtc attgcagaca
     3361 tcggcttttt tatcattaac gataatttac aatcacttca ttccgttgaa tctttagcgg
     3421 cggagttagc cgcccaccgc caggcacttc ccagataaag cgcaacggtt ctgccgccgg
     3481 tataccggaa aaggccatgg tggttccact ctgcccctcc aattcgacac aacgagattg
     3541 tgaacacaag cgaacccgca gtcctgcagg tgtcggaccg ataagctgat aacgccacgc
     3601 caccagcgtc atcaatcctg aagcaggttg tcgcgtagaa agaggcgctg acgacatcga
     3661 ttcaccgcga tgatttagcg taacgcccac gctactcgcc tgccacatcc cctccccggc
     3721 ggcttgcacc agcagcggaa acaataatat tgctaataag gctctcatta tttgccgcca
     3781 atcgtcgccg tcatacggat atgtcggtta tctgatagtt ccagattcga cagcaccact
     3841 aactgcggca agctgcggcg caggaagcga gacaataatg gtcgtagcgc gtggttcacc
     3901 aacaataccg gcggcgcacc caacatctcc tgacgggata gcgcttcctg agtttgcgcc
     3961 agtaaacgat ccgccagccc tggctccagt ccaccaccgc cttgcaacgc ctgtagcaac
     4021 aaacgttcca gcggtgtatc gaggccaata acatggactt catctttgcc aggaaaccac
     4081 tgctgggtaa tcgcccgtcc caacgctacg cgcacgacag cggttaattc atgcggatcg
     4141 ctttggatgg gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggc
     4201 actttttcat cgagcagatt ttgcagcact ttatgcagtg tggtaagcgt gacgacgcca
     4261 ggaacgagat cttccgtcag ctttggcatc tcctgggcga cgcgatccag cagctgttgt
     4321 gcctcctgac gaccaaacag ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt
     4381 gccaccaccg tgctggcctc aaccacggtg tacccctgaa tctgcgcctg ttctttaagc
     4441 gcactttcaa tccagatagc attcaggcca aatgccggat caacggtcgc ttcaccaggt
     4501 aatgttccgg cggcagtgcc tggattaatt gccagccagc gccccggata agcatcacca
     4561 ctccccattt cgacaccttt catcaagata cgatagcggg ctggttgcaa atccatgtta
     4621 tcgcggatgt gcaccactgg gggtaggaag cccatctcct gggcaaattt cttgcggata
     4681 ctgcgtatac ggcccaacaa ctcaccatcc tgctgaaaat caaccatcgg gatcaaccga
     4741 taccccactt ccattcccag agaatcttcc agttgtacat cgttccacgt tgcttcgact
     4801 accgaattat tctctgccat ttttaccggt tttggttcgg caggcgcttt ttgttcacgt
     4861 ccgcggatcc accaggccag ccccagcaat ccggcagtga acagcaaaaa taccaggttt
     4921 ggcattccag gcaccaggcc gagtaaaccg agcacggcgg cgcttaataa cataacgctt
     4981 gggttactga aaagctgatt caccatctgc tcgccaacat cctgatcggt gctgacacgc
     5041 gtaacgatga cccccgcggc ggtagaaatc accaacgccg gaatttgtgc caccagaccg
     5101 tcgccaatgg tcaatagcgt ataactttcc gccgcgtgcc ccatgctcat gccatgttgc
     5161 agcacaccga ccagcaatcc gccgacaacg ttaatgacca tgatgaggat cccggcgatg
     5221 gcatcgccgc gaacaaactt acttgccccg tccatcgagc cgtaaaaatc ggcttcctga
     5281 gtcacttcgg agcggcgttt tttcgcttca tcttcaccaa tcaatccggc gttaaggtcg
     5341 gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc gcccacttcc
     5401 gcaatacgcc cggcaccttt ggtgataacc ataaagttga tgatcacgag aatgacgaac
     5461 accacgatac cgatagcgaa attgccaccg acgaggaagt gaccgaacgc ttcgaccacc
     5521 tttccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt tgaagccacg
     5581 ttaagtgcca gacgcaacag cgtggtaaac aacagaatgg tcggaaacgc agcaaactcg
     5641 agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag
     5701 gtaaacagca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac
     5761 aggatcaaaa tcggtccggc aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg
     5821 cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggcttc
     5881 cggcaccgga agatgagtag gttgtacagg gcgctgtcca ccagccagac gccagcgttt
     5941 cagttgccag acccaggcca gcacttccgc caccgcggcg tacagttgac ccgggatttg
     6001 ttgaccgatc tccgcatgtc gatacagcgc acgcgccagc ggcggcgctt caagcgtcgg
     6061 gacgttattt tcagcgccaa tttcacgaat gcgcagcgcg accagccctg cacctttagc
     6121 gaccactttc ggtgcgctca ttttgttttc gtcatactgc aacgctaccg aatagtgggt
     6181 cgggttattg acaatgacat ccgctttcgg cacatcggcc atcatccgac gccgtgcggc
     6241 cgctcgctgc atctgacgga tccgcccttt aacatggggg tcgccttcgc tttgtttgaa
     6301 ctcatcacga atatcctgcc gtgacattcg cagctttttc aggtggctga agatttggaa
     6361 aaagacgtca aatcccacca ttggaatgac accaagcacc accagcagcg cgcatagccc
     6421 taccagatcc attgcattac ccatggcggt aatcggagac tcggccatta agcgcatcat
     6481 ctgcggccag tgataccaga gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa
     6541 aattgcctta agcaactccg cgccagtctg tgccgagaac gtccgtttaa tgcccggtag
     6601 cgggttgagt ttggaaaact tcggctgcaa ggattttccg ctaaatacca gcccccccag
     6661 cataaccgga gaaataatcg ccaccagcac cacgccgcta atcagcggta gcagcgccag
     6721 catggcttct ctgatcagca gaataatctg cccgaggatc agattcgggt cattgataat
     6781 actgtgatca aaatgtagcc ctgcggagag catgcccgac aatcgacggg ccagcgacac
     6841 accgccaaac cagataacac taacgcccac cagcaaaatc agcagtgaag tcagttcacg
     6901 ggaacgcggg atttgcccct cttcccgcgc tttttctagt cggtgaggtg tgggggcctc
     6961 tgttttgtcg tcgctctcgt cagacacgtc gccaatcctg gaaagagtat taagcgtaaa
     7021 tgatgccaga gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag
     7081 ggcttttact gccacacatc aagcatagta tgcgtttgtc ggatgcggcg tcatcgcctg
     7141 atatgacgtg gtcacgtcac atcaggcaat acaaatcaaa atccaagact atccaacaaa
     7201 tcgtccacct gaccctgact ggctaccaca ccggctttgc tggtatcgac ctgaggtcca
     7261 ttaagcaaac tctggttttc acgttttgga cgcgactcct gttccgggat gttttccaac
     7321 aacaccatca gcaactgacg ttcgatctcc tgaatgacat ccatcatccg cttaatgacc
     7381 tgcccggtga gatcctgaaa atcctgcgcc atcatgattt ccagcagttg cgcgttagta
     7441 aagctggtat gcgcggggac atctgccaga aattgccgtg tatcgatcac cagttcacgg
     7501 gcgtcggcaa ggtcaatcgg atcggcaaac cagtcatccc aacgttgggt taacgctttt
     7561 gctgatttct ccatttgatc ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc
     7621 tccgcagcct gggcggtcat ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg
     7681 gcttccgctg cttcggcaat ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc
     7741 atacgcgtca ggctgccgat gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt
     7801 ttgattgatg gttgcatcat agtcgcatcc tcacatgccc agtttctcaa agattttgtt
     7861 gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc acatagccac tggcccccgc
     7921 ttgcgccgca gcaatgatgt tctctttctt cgcttctgca gtcaccatta acactggcaa
     7981 tgccgacatc gcgccatccg cacgaatcgt tttcagcaac tccaggccat ccatattggg
     8041 catgttccag tcggagataa caaatccata accgcctgcc tgcaacttat tgagagcgtc
     8101 gacgccatct tccgcttcct caacattatt gaatcccagc tctttcagca ggttacgcac
     8161 tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat ttaagttctt tatccgccat
     8221 ttcacactcc tgatttaaat acgtatcgcc tgtccggcac taatttttgc cagcatttgc
     8281 tgacttacct ggctaagatc gaccacttcg cagacaccac ccatattgat ggcctcgcgc
     8341 ggcatgccga acaccacgca acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga
     8401 cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac cggttaggat taccccaacc
     8461 gcattacgcc ccgcctgttt ggccacagaa tggaacaaca catctaccga tggccgatgg
     8521 cgattaaccg ccgggccatc gtgaattttg atttgataat ttgcgccact acgcgccagc
     8581 tccatatgcc gatcgcccgg cgcgatatag gcatgtcccg gcaagacgcg ctctccatct
     8641 tcggcttctt taaccccgat ctggcaaagc ttattaagtc tgtcggcaaa agagcgggtg
     8701 aaaccgggcg gcatatgctg ggtaattaac agtgccggac tggaaagcgg caacggttgc
     8761 agtacgtgac gaattgcctc agttcccccc gttgaagcac caatcgcaat cagtttttca
     8821 gaactcaaca acggccccgc cttcagcgtt gtcggtgccg acaatggctt atgtgctgca
     8881 aggctcgcct ttgctgccgt acgcaccttt tcagcaatca tttcgctata cgccagcatt
     8941 ccttcgcgaa tacccagttg cggtttggtg acaaaatcta tcgcccccaa ctccagcgcg
     9001 cgcagcgtga cttctgaccc tttgccggtc agggaagaaa ccatcacaac gggcattgga
     9061 cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca tccgcggcat ttcaacatcc
     9121 agcgtcaata catcgggatt gaatttctta atcaaatcac gcgcaaccag cggatcaggg
     9181 gcggtcgcca ccatttccat gtcgctatgg ctgttgatga tttctgtcat gatctggcgc
     9241 atcagtgccg aatcatcgac agataacacc ctgattttgc tcatcgttaa tccttactta
     9301 gcgcatacac cgtctgacca cgcagcgtga agcggcgctc aaggtggcta aagttttcag
     9361 agtgacccgc aaacagcaat ccgtcgggtt taaggagcga aacaaagcgg cgcaaaatct
     9421 cctgctgggt agtttgatcg aagtagatca tgacgttacg acagaagatc gcatcaaacg
     9481 gccccggcac ggtgtactgt ttcgccagca aattcagcgg ggcaaaatca acatagttcg
     9541 ccagctcctg acgcacgcgt accagccctt catgcggtcc cgtccctcgc atgaaatacc
     9601 gttgcagttg ctgcggcgtc aggtttttca actcttcatg gcgataaata ccgcttctgg
     9661 ctttttccag cacttcggtg tcgatatcgc tggcaaacac tttccagcgt ccgggcgcgg
     9721 tgcccaatgt atcagccagc gtcatcgcta tgctgtacgg ctcttcgccg gttgaagccg
     9781 ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc gtgatccgcg agcagaggaa
     9841 aatggtgtgc ctcacggaaa aatgccgtca gattcgtggt cagcgaattg ataaacgcct
     9901 gccactcacc gctgtgctga ttagattcca gcaagttcag gtaatgaccg aaatccgcca
     9961 gtcccagcgc acgcaaacga cgaaccagtc ggttgtaaac catgtcgcgt ttatggtcag
    10021 ccagaacgat cccggcgcgt tgatagatca attgacttat ccgccgaaaa tgcgcgtcgg
    10081 aaagcgccag gcgctcggtc atctgtaaca ataaagacgt ttgcccacag ggcagagatg
    10141 aagtcatagc gccttctcaa tcacttcagg ataccactgg cgcaatttgt aactgcgccg
    10201 actcatgtcg tgctacttca tgttcttcaa gggtaaatac cgccacacgc gacgaaagat
    10261 ggtcggcctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaagaca tgccgttact
    10321 gccaatctgc aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat
    10381 acgattaaac atcaacgctc cccatcaggc ggcaatgacc gctttagtaa atactcgtca
    10441 aaatgtttcc cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg
    10501 ctcactggca ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg
    10561 gaacgcggaa accgcttgcg ttaaacgact cgcctgttct tccagcgcag ctgcggcggc
    10621 agctgattcc tgcaccagcg atgcgttctg ttgcgtgacg cgatccattt ccgaaaccgc
    10681 caatgcgact tgatcgatgc cacgactctg ttcatccgat gctgatgcaa tctcgcccat
    10741 aatgtcagtc acgcgagtga cagcattgac gatattgttc attgtttccc ctgcgctttc
    10801 gaccagcacc gaaccggtat caacgcgcga gacggagtct tcaatgaggg ctttgatctc
    10861 ttttgccgcc tgggcgctgc gactggcaag attacgcact tcgcccgcca ccacggcaaa
    10921 accacggcct tgttcacccg ctcgcgcggc ttcaacagcg gcattcagcg cgaggatatt
    10981 agtctggaag gcaataccat cgataacgct gataatgtcg gcaattttct tcgaactatc
    11041 ggcgatctca tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt
    11101 gtcggaggca ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac
    11161 tgtcgcggcg agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc
    11221 agtacgggag gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc
    11281 atctgaacct tcgcgcacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac
    11341 gctctgcgcc aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt
    11401 accaccggca atttcgcgaa tatgcgcaat aatttttgcc agcggtgtaa gcaacatacg
    11461 gcgaatgccg taccacgccg ccagcagaat caataccacc accagcgcga taaccgccag
    11521 ttgccactgg gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt
    11581 ttcactgctg agggcgtact gagcaaacgc ttctcccatt gcattttgca ttccctgggt
    11641 tggctgagcg aaataagctc cagtattgcc ataatcaaga taatcaatca gttcagttaa
    11701 cgctgtgtga tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg
    11761 taacggtgcc atgcttttga attttttata atgagtcgcg gcctgggcca atgttttcct
    11821 ggcgctatcg agcaattcaa ctttggcatt actttgctga ttggatgaat ccatcatcat
    11881 ccgcaccgct gaacgactca ggttaatgcg cgtttgtagc attaaatccc atgttgacgt
    11941 cagctcgctc tgctgttccc gtaattgatt ggaaaccaca aagctcttct ggctatggtg
    12001 aagggaagaa aaaa
//
LOCUS       DAOFSV010000136        11948 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.136, whole genome shotgun sequence.
ACCESSION   DAOFSV010000136 DAOFSV010000000
VERSION     DAOFSV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.136"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(6..1364)
                     /gene="mltD"
                     /locus_tag="QBH27_003787"
     CDS             complement(6..1364)
                     /gene="mltD"
                     /locus_tag="QBH27_003787"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDQ7016049.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1436..2191)
                     /gene="gloB"
                     /locus_tag="QBH27_003788"
     CDS             complement(1436..2191)
                     /gene="gloB"
                     /locus_tag="QBH27_003788"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDQ7016050.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2225..2947
                     /locus_tag="QBH27_003789"
     CDS             2225..2947
                     /locus_tag="QBH27_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ7016051.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(2944..3411)
                     /gene="rnhA"
                     /locus_tag="QBH27_003790"
     CDS             complement(2944..3411)
                     /gene="rnhA"
                     /locus_tag="QBH27_003790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDQ7016052.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCKVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            3476..4207
                     /gene="dnaQ"
                     /locus_tag="QBH27_003791"
     CDS             3476..4207
                     /gene="dnaQ"
                     /locus_tag="QBH27_003791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDQ7016053.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGTCLWRA"
     gene            4340..4416
                     /locus_tag="QBH27_003792"
     tRNA            4340..4416
                     /locus_tag="QBH27_003792"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4374..4376,aa:Asp,seq:gtc)
     gene            complement(4515..5285)
                     /gene="yafV"
                     /locus_tag="QBH27_003793"
     CDS             complement(4515..5285)
                     /gene="yafV"
                     /locus_tag="QBH27_003793"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDQ7016054.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            5466..5912
                     /gene="ivy"
                     /locus_tag="QBH27_003794"
     CDS             5466..5912
                     /gene="ivy"
                     /locus_tag="QBH27_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDQ7016055.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(5955..8399)
                     /gene="fadE"
                     /locus_tag="QBH27_003795"
     CDS             complement(5955..8399)
                     /gene="fadE"
                     /locus_tag="QBH27_003795"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDQ7016056.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            8639..9217
                     /gene="lpcA"
                     /locus_tag="QBH27_003796"
     CDS             8639..9217
                     /gene="lpcA"
                     /locus_tag="QBH27_003796"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDQ7016057.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9422..10189
                     /locus_tag="QBH27_003797"
     CDS             9422..10189
                     /locus_tag="QBH27_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDQ7016058.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10160..10900)
                     /locus_tag="QBH27_003798"
     CDS             complement(10160..10900)
                     /locus_tag="QBH27_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDQ7016059.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            11212..>11948
                     /locus_tag="QBH27_003799"
     CDS             11212..>11948
                     /locus_tag="QBH27_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDQ7016060.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKANDRRWQA
                     LAENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTE"
BASE COUNT         2889 a         3112 c         2973 g         2974 t
ORIGIN      
        1 gtttgtcagg aatctggcat gttgttgttt ttcacaaaca acgtcagctt atcgcctggt
       61 tgcagattcg cagtgtcgct gttccagcgc attacatctt tgatgttcac gccgtggcgt
      121 ttagcaatgc ttgaaagcga atcgcctttg cgcacacgat acgtaatgct atcgctgttg
      181 tttgccagtc gctgtgcgct actgcctgca ccaatcgtca aactttggcc tggcttcagc
      241 ttagatccgc gcagtttgtt ccactgctgc aaatctttgg tgcttacgcc gagacgtgaa
      301 gcgatacttg aaagcgtgtc gccagagcgt acggtgtaaa cacggctgtt aagtggcgta
      361 ttgtcggcaa ccagcgtcga ctgtacagca gcaatttcgc ctgaagccag agattcacgc
      421 agttgatctg catgcttctt tggcaccatc acgtactgcg gaccacttgc gcccagcgtg
      481 gagcctttca cgcctgcgtt gaatgtcttc agcttgctga cggaaatccc cgccatatct
      541 gcaaccttcg ccatttcaac cgggctgctc aggtgcacac gtgccagagc acggctttca
      601 tcggtcgttg gcagacgtac gccataacgc ttgctgtttt tgagaatatc actcaatgcc
      661 agcattttag gcacgtactg cttcgtttcc tgcggcaacg gtaacgacca gaagtccgtg
      721 gatttcccac gcgctttgtt cgttttaatt gccttcatga cccgaccttc gccgctgtta
      781 taagccgcta cggtcagaag ccagtcgccg tcaaacatct tgttcagacg ctgcatcatg
      841 ttcagcgcgg cagttgttga agcaacaaca tcgcgacgcg cgtcataatt gcgggtctgt
      901 ttcaaaccat aattgcgccc cgtgctcgga atgatctgcc agatgcccgc ggcattggcg
      961 ccagacgttg cgtgaggatc aaaagcgctc tccactatgg gtagtagtac cagttccata
     1021 ggcatgttac gttttttaac ttgcccggct atccagtaca tatacggctc tgcccgtaaa
     1081 gttacatcgt ggagatagct cttattgcgt aaatatttct gtttctgttc gcgaatccgg
     1141 tcgttttccg gaattcccat ctttagctcg tcgccaatga aagcccacaa gtcaccatct
     1201 ggcgcgatag acgtcccatc gtccatccat cgtgcctgac ttgtaaactt tgctgcttcc
     1261 ccttgaccag ctgcagaaag gctctgtgcg tgctgttgaa cgttgccggt gctctggcaa
     1321 cccacgagca ggacagaggc gagtaatatc gcttttgcct tcatgtgtgt gtcaatagtt
     1381 gcttaaaaga cgaccgatca taacggcgaa cggagccgat gacaagaaag ttttatcaga
     1441 acctatcttt ctttgacctt aaccatgcaa aacgttcttc aggttgttgc aataatgttt
     1501 cttcattaat tacattaatt aaatcaatat cttccgttct taaaaaaaca ttaatttgcc
     1561 gctcattttt cagaattacg ggtagtgtta tttgattttt tgcccgtaac tccttaactt
     1621 tacgataata atcatttatg gacaaatcgt gcggaagaat actcaaagca aacttcatat
     1681 ttgataaggt atattcatga gcacaacata ccaatgtgtc gtcaggtaac gcacttaact
     1741 ttctaagtga ttgatacatt tgtgatggtg tcccttcaaa caaccgacca cacccaccag
     1801 aaaacagcgt gtcgccgcaa aatagataag gtttactgaa gtaacagata tgtcctaaag
     1861 tgtgacccgg tgtggcaatt acactaaatt catgccccaa aacgaaggca gtttcgccat
     1921 ctttgactac ctgtgttgtt cccttatctt gtgtctcttg tggtccatac accacaattt
     1981 gcggaaactt ttccaccagt tcttttacgc cgccaacgtg atcgtggtga tggtgggtga
     2041 gaaatatggc ctccggttgc cagttattgg cggaaatggc gtttaatact ggctctgcgt
     2101 ccccgggatc gacaatcaga cagcgacctg cttcatcatt caaaacccag atgtaattgt
     2161 catcaaaggc gggaatactg ttaagattca tagattacct ctcagtgtga aacggaaggt
     2221 tgtgatgaaa ccggcaagag tccctcaaac tgtcgtggct cctgattgct ggggcgattt
     2281 gccctgggga gagctttatc gcaaggcgct ggagcgtcag ctcaacccgt ggttcactaa
     2341 aatgtatggt tttcatctgc ttaagattgg caatttaagc gcagaaatca attgcgaagc
     2401 gtgcgcggtt tctcatcaag tgaatgtttc tgcgcaagga atgcccgtcc aggtgcaggc
     2461 ggacccactt catcttcctt ttgccgataa atccgttgat gtttgtctac tggcacatac
     2521 attgccgtgg tgcactgatc cgcatcgttt attgcgtgaa gccgatcggg tactgattga
     2581 tgatggttgg ctggtgatta gtggcttcaa tcccatcagt ttaatgggat tacgcaaact
     2641 tgtgccggta ttgcgcaaaa catcgcccta taacagccgg atgtttactc tgatgcggca
     2701 gctggactgg ctctctttgt tgaattttga agtgctacac gccagccgtt tccacgttct
     2761 cccgtggaac aaacacggag gaaaactatt gaatgcgcat attcctgcgc ttggttgctt
     2821 acaacttatt gttgcccgga aacggactat tcctttaacg ctaaatccga tgaaacagag
     2881 caagaataaa ccgcgaattc gccaggcggt tggtgccacc cggcaatgtc gtaaaccaca
     2941 ggcttaaact tcgacttgat agcctgtatc ttccagtgtg ggattcattg ccgcagcacg
     3001 agccagttca tcacagcgtt cgttttccgg gtgtccggca tggcctttaa cccattccca
     3061 tttgatttga tgctgcccca gagcagcatc aagacgttgc cagagatcga cattttttac
     3121 tggttttttg tctgctgttt tccagccacg ttttttccag ttatggatcc actgggtgat
     3181 accctggcgg acatactggc tgtcggtact caaaatgact ttgcaatgtt cttttaacgc
     3241 ctccagcgcg acaatagcgg ccatcaactc catacggttg ttggtggtgc gggtgtagcc
     3301 agcgctaaag gttttctcgc gtccgcgata gcgtaaaata gcgccgtaac ccccaggtcc
     3361 aggattaccc agacacgaac catcggtgaa aatttctacc tgtttaagca tctctggtag
     3421 acttcctgta attgaatcga actgtaaaac gacaagtctg acataaatga ccgctatgag
     3481 cactgcaatt acacgccaga tcgttctcga tactgaaacc accggtatga accagattgg
     3541 tgcgcactat gaaggccaca agatcattga gattggtgcc gttgaagtgg tgaaccgtcg
     3601 cctgacgggc aataacttcc atgtttatct caaacccgat cggctggtgg atccggaagc
     3661 ctttggcgta catggtattg ccgatgaatt tttgctcgat aagcccacgt ttgccgaggt
     3721 agccgatgag ttcatggact atatccgcgg cgcggagttg gtgatccata acgcagcgtt
     3781 tgatatcggc tttatggact acgagttttc gttgcttaag cgcgatattc cgaagaccaa
     3841 taccttctgt aaggtcaccg atagccttgc ggtggcgagg aaaatgtttc ccggtaaacg
     3901 caacagcctc gatgcgttat gtgcccgcta cgaaatagat aacagtaaac gaacgctgca
     3961 tggggcatta ctcgatgccc agatccttgc ggaagtttat ctggcgatga ccggtggtca
     4021 aacgtcgatg gcttttgcga tggaaggaga gacacaacag caacaaggtg aaacaacaat
     4081 tcagcgcatt gtgcgtcagg caagtaagtt acgcgttgtt tttgcgacag atgaagagct
     4141 tgcagcacat gaagcccgtc ttgatctggt gcagaagaag ggcggaacct gcctctggcg
     4201 ggcataaata cctgtaaaag gcgctaaaaa tagcgacttg ggcgattttt gcagcaaacg
     4261 attcaaaaga tgagaaaaac cgttgacgaa gggtgaggca atccgtaata ttcgcctcgt
     4321 tcccaacgga acacaacgcg gagcggtagt tcagtcggtt agaatacctg cctgtcacgc
     4381 agggggtcgc gggttcgagt cccgtccgtt ccgccaatat tcagaaagcc cgaatcatta
     4441 ctgattcggg atttcgtcgt ttaagttcca gagaaaaata gccacgaaac ttatctcgta
     4501 gccatattca ccctctaacg aaatgtgaac tcatccgcat cctgccatgc cggaaacttt
     4561 tctctatatt cccgcaatgc catcatcgat agctccgcat caatgcgcgt tgcctgatgc
     4621 gcgtcggcag tagcgataat ctcgccttgt ggattaatca cccggctgtc accgcgataa
     4681 tggcagccgt tgccatcgct gccgacgcga ttacatcccg ctacatacgc ctgattctca
     4741 atcgcgcggg ctgtcagtaa tgcctgccag tgcagagagc gcggtgcagg ccagttggca
     4801 acgtagatgg cgaggtcata atcgttgaga ttgcgcgacc acaccggaaa acgtaagtcg
     4861 tagcacacca gcggtaaaat acgccagccg cgccattcca caatcactcg ctcattgcct
     4921 gctttataat gcagatgctc atctgccatg cggaacagat gacgcttatc ataaaaatgt
     4981 accgtgccgc ccggctcaac cagcaaaaag cggttaaccg aatcagactc cgtttgtaat
     5041 gcaacactgc ctgcaatcag cgcattgcac tgctgcgctt tagcggtcat ccagttcact
     5101 acgtcatctt gtgctagcga cgaagctgcc gcttccatgg caaagccgct ggtaaacatc
     5161 tccggtagaa cgatcacatc gcgcccggta ataccttcca gttgacgatc aaaatggcgc
     5221 aggttggcag gaccatccat ccacaccagt ggttgctgca aaagcgtaat cttcaaacca
     5281 ggcacggtgt acaactcctt tatgcgaagg gttttataac tctaacacct tatcaggcag
     5341 ttgccttagc gcagaataaa ttgataacaa atgctgatat tggaaatatc tgatttgcaa
     5401 attatcgtgt tatcgccagg ctttagggag ttaatcacat gggcaggata agcccgggag
     5461 gaatgatgtt taaggcaata acgacagtcg ccgctctggt catcgccacc agtgcaatgg
     5521 cgcaggatga tttaaccatt agcagtcttg caaagggcga aaccaccaaa gcggcgttta
     5581 atcagatggt gcaagggcat aagctgcctg cctgggtgat gaaaggcggt acttatactc
     5641 ccgcacaaac cgtgacattg ggagatgaga cgtatcaggt gatgagcgcg tgcaaacctc
     5701 atgactgtgg ctcgcaacgt atcgctgtga tgtggtccga gaaatctaat cagatgacgg
     5761 ggctgttctc gactattgat gagaaaacgt cgcaagagaa actcacctgg ctgaatgtga
     5821 atgatgcgct ttcgattgat ggcaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg
     5881 aaaaccatcc ggatggcttt aattttaaat aattagccga taaaaaaacg gagcctttcg
     5941 gctccgttat tcaattacgc ggcttcaact ttccgcactt tctccggcaa ctttaccggc
     6001 ttcgtcgcca gctcttctgg atcaaagtca tcaacgttaa tactacgcag acggctttct
     6061 tcagctttca ccagaatagc ggcttcatct ttatcaatca gccccttcgt cagcgcgttg
     6121 tgcgccagtt catccagacg ggtaaatggc agatttttac ccagctcttt acagatccgc
     6181 tgatgaatcg ggtcggcggc aatcacatcc accaacgcct cttccagtaa accaaccggg
     6241 ttatgctcgc ttggagtcag gtactgaccg cgaccaatac gtgaacgggt ggcattcggc
     6301 acttgtaaaa tcttcgccac cttatgatcc agcttgtcag acggtgccag ataatgacgt
     6361 ccggtcggga agatcaccac attcagcagc ccggcaacca cgcggttcgg gaagttttgc
     6421 agtaaatcgt ccatcgcctg ttctgcctga tacagcgcat cctgtacgcc ccagtgcacc
     6481 agcggcaggt cggcttcatt acggccttcg tcgtcataac gcttcagaac ggcagaggcg
     6541 aggtaaagct ggcttaaaat atcccccaga cgggctgaga tgcgctcacg acgtttcagg
     6601 ctgccgccca gcaccgccat cgagacatca gaaagcaggg cgaggttggc gctcaggcgg
     6661 ttcaggtgct gatagtagcg tttagtggca tcgccggttg gcgtgctgct ggttaaaccg
     6721 cgcgtcaggc ccagccagaa gctgcgaact ttgttgctac cgacgtgacc gatatgtttg
     6781 aacagcagtt tatcgaacgc gtttacgtca ttgttcttcg ccgcttccat ctcttccagc
     6841 acgtacggat ggcaacgaat cgccccctga ccgaagatca tcatgctacg ggtcagaata
     6901 tttgcccctt caacggtgat ggcaatcggt gccccctggt aagcacgggc caggaagttg
     6961 ctctgcccga gcataatgcc tttacccccg gtgatatcca ttgcgtcgat aatcgattgc
     7021 tgcccgcggt gggtacagtg atacttaacg atagccgaca gcacggcggg tttttcaccg
     7081 agcataatgc cgtaggtaat cagcgatgcc gcagcatcca tcacgtaggc gttcccggca
     7141 atacgcgcca gcggctcttc aatcccttcc atcttaccaa tagaaatttt gaactgacgg
     7201 cgaatgtgcg catacgcacc cgttgccagc gctaccgatt tcacgccgcc ggttgagttg
     7261 gaaggcaggg tgatgccgcg gcctaccgag aggcactcca ccagcatccg ccagccttgc
     7321 ccggccattt tcggcccgcc gatgatgtaa tcgatcggca cgaacacatc tttaccgcgc
     7381 gtcggtccgt tctggaacgg tacgttcagc gggaagtggc gacgaccaat ttccacgccc
     7441 ggcgtggtgg tcgggatcag cgcacaggta atacctaaat cttctgcacc gccgagtaat
     7501 ttctccgggt cggagagttt aaacgccaga cccagcacgg tcgcaatcgg tgccagcgta
     7561 atgtagcgtt tgttccaggt cagacgcatc cccagcacct gctggccctg ccattcgccc
     7621 atgcagacaa tcccggtatc cggaatcgcg cccgcatcgg aacccgcttc ggggctggtc
     7681 agtgcaaagc aagggatctc ctgaccacgc gccagacgcg gcagatagtg atttttctgc
     7741 tcgtcagtgc cgtaatgttg cagcagctcg cccgggccta atgagttcgg cacgccgacg
     7801 gtaattgcca ggatcccgct cacgccggag agtttttgca gcacacgaga ctgggcataa
     7861 gccgagaact ccagcccgcc gtactctttt ttgatgatca tcgcgaagaa acgatgctct
     7921 ttaaggtacg cccacaactc cggcggcagg tccgccagct catgagtaat ctggaaatca
     7981 ttcgccatcc ggcaggcttc ttctaccgga ccgtcgagaa acgcttgctc ttcagcggtc
     8041 aggcgcggct gcggatagtt atgcagcttt ttccagtccg gcttgccctg gaacaagtcg
     8101 ccctcccacc aggtggtgcc cgcatcaatc gcttctttct cagtgcgcga catcggcggc
     8161 atcaccttac ggaaaccgcg aaataccggt gcggaaatca tcgacttacg cataggcgca
     8221 aagttaaatg gcacgaggat aatggccaga ggcactagta cccacgccga ccacagaccc
     8281 gcaacgccga gggcggctgt ccaggcgagc aaaatcagac tgctgataaa taagctcacg
     8341 cggtgataga acaacgcgcc gagcaggaca accgtagcga gaatactcaa aatcatcata
     8401 acgaaaagcc ccttacttgt aggaggtctg accacttgtg atgatatggt tgtagtggat
     8461 gtaaaaacat ttagcaatgc gtttacaata taattacaac aaagctcaca ttgttgctgt
     8521 ttttatccgc acttcaggtc aaaaagtcct ggtcatagca cctgcccgta cttctcgctt
     8581 ttggcggtat ccggtacact gcattttgtc tattacattt atgctgaagg atatcctcat
     8641 gtaccaggat cttattcgta atgaactgaa cgaagcggcg gaaacgctgg ctaacttttt
     8701 aaaagatgac gccaatattc acgccattca gcgcgcggcg gtcctgttag cagacagctt
     8761 taaagccggt ggcaaagtgc tttcctgtgg caacggcggc tcccattgcg acgccatgca
     8821 ctttgccgaa gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc
     8881 catttctgac gttagtcata tttcctgcgt cggtaacgat tttggtttca atgatatttt
     8941 ctcccgttac gttgaagcgg taggtcgcga aggcgatgta ctgctgggga tctccacctc
     9001 cggtaactct gcaaacgtga tcaaagcgat cgcggcggcg cgtgagaagg ggatgaaagt
     9061 gatcaccctg accggtaaag acggcggcaa aatggctggc acggcggata tcgaaattcg
     9121 cgtaccgcac tttggttatg ccgaccgcat tcaggagatc cacattaaag tgatccatat
     9181 cctgatccag ttaattgaaa aagagatggt taagtaagtc tggcgtaggc cggataaggc
     9241 gtttacgccg catccggcat ttgtgctctg atgcctgatg cgacgctgac gcgtcttatc
     9301 atgcctacaa tctgcacccg aaccgtaggc cgaataatgc gttcacgcca catccgacct
     9361 gaaaattctt aaatcaatct tcgccggggg ccatgcgctc ccgctgttgt ggaggttacc
     9421 catgtgcgaa ttgctcggga tgagtgccaa cgtccctacc gatatctgct ttagtttcac
     9481 cggacttgta cagcgtggtg gtggaaccgg gccacataaa gatggctggg gcattacctt
     9541 ttacgaaggt aaaggctgtc gcacatttaa agatccgcaa cccagcttta attcccccat
     9601 cgccaaactt gtccaggact acccgataaa atcctgttcg gtggtggctc atattcgcca
     9661 ggctaatcgg ggcgaggtgg cgctggaaaa tactcacccg tttacccgcg agttatgggg
     9721 gcgtaactgg acttatgccc ataatggaca actgacgggc tacaaatcac tggaaaccgg
     9781 caacttccgc ccggtcggtg aaaccgatag cgaaaaagcc ttttgctggc tcctgcataa
     9841 attaacgcag cgttacccgc gcacaccggg caacatggcg gcagtgttta aatatatcgc
     9901 ctcactggcg gatgaactgc ggcagaaggg cgttttcaac atgctgcttt ctgacgggcg
     9961 ctatgtaatg gcgtattgct cgactaattt acactggatc acccgccgcg cgccgtttgg
    10021 cgtggcaacg ttgctggatc aggatgtgga aatcgacttc agctcgcaga ccacaccgaa
    10081 tgatgtggtc acggtgattg cgacacagcc gctgacgggc aatgaaacct ggcaaaagat
    10141 tatgccaggt gaatggcgct tattttgcct cggggagcgt gtagtttgat gccagttgtg
    10201 gctgcacaac ttcgtggctt aacggcttgc tgaccacgta acggccattg accacagaaa
    10261 cggtcggtgg cttacgggtt tgctcaaagt agtcgtagcc cggcttcagt tgctcccaga
    10321 agtccttaaa gttggaatat ttatggcgct tcatattggc gtcggtcatg cggaacggat
    10381 aaatactcac ttgcacgctc ggctgaccaa acaccagcgc accagtaacg aactggaaga
    10441 tctcatcaat accctgattg gtcatcgcgt agcagccgat ggaaacacaa tcgccgtgaa
    10501 tcatcaggta tttcccttca taaccatgcg cacggtcata ggcgtttggg aatccaatat
    10561 taatcgcttt gtagtaacgg ctgtctggtt ttaactgatt acgctggacg ctataaaacc
    10621 cttccgggct tttgaaatcg ccctgacgct gttttggccc taagccgccg gaatatttac
    10681 agattttata gctgtcgagc agttgatatt gctcgcccat tttgacgtag agatcgagcg
    10741 tacgttcttc cttgaagatc tggatgtaga caggggatcc catcaactgc tgcttatact
    10801 ctttgctcac tggcgtggtc gagctactgc tgcccagcaa accggcaaac gaaacgcatg
    10861 ggatcaacaa catcgcaaga attaatgcga ttttacgcat actgcttatt ccttgataaa
    10921 acggttacac acgccaggac ggcaaaatga atcccaaatc ggaatagtct ggatttggaa
    10981 ggctcacatt atcaccaaaa gagttttacg caagcctgtc ggcgcagggt ttacaaattt
    11041 agcacaggtg cagttttcaa gagtccgaaa agcgcatgga ttgtagattt atttcttatt
    11101 tcctaagatt atgaaaccgc aatagtaata tgcaaataag caattaataa tcccgtttac
    11161 tcctccggtc ttttaaaatt ctcaccttca ttcagctggt ttatgtcctt catgtcttcg
    11221 ttcttgcacg gcagatttct gcatccaggc gtgttttcgt tatgcgtttt gctcccttta
    11281 ctcgccagcg ccaccacatc acatatctct tttagctacg ccgcccgcca gcggatgcaa
    11341 aaccgtgcgc gtttattaaa acagtaccaa acccatctga aaaagcaggc cagctatatt
    11401 gtggaaggca atgctaaaag cagaagggcg ctgcgccagc ataatcggga gcagataaaa
    11461 cagcatccag aatggtttcc tgcgccgctc aaggcgaacg acagacgctg gcaggcgctg
    11521 gcggaaaaca atcatttcct cagcagcaac cacctgcata acatcaccga agtggcgatt
    11581 caccgcctgg aacagcaact tggcaagcct tatgtctggg ggggcacgcg gcctgataaa
    11641 ggtttcgact gtagcgggtt ggttttttat gcctacaaca agatccttga ggctaagctc
    11701 ccgcgcacgg ccaatgagat gtaccactac cgtcgggcaa cgattgtggc gaataacgac
    11761 ctgcgccggg gagatttgct gtttttccat atccacagcc gtgagatagc cgaccatatg
    11821 ggcgtatatc ttggagacgg tcagtttatc gagtcaccgc gcaccgggga aaccattcgg
    11881 gtaagtcggt tagctgaacc tttctggcag gatcattttt tgggcgcgcg gcggattttg
    11941 acggaaga
//
LOCUS       DAOFSV010000137        11773 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.137, whole genome shotgun sequence.
ACCESSION   DAOFSV010000137 DAOFSV010000000
VERSION     DAOFSV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.137"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            447..1535
                     /gene="pdeL"
                     /locus_tag="QBH27_003800"
     CDS             447..1535
                     /gene="pdeL"
                     /locus_tag="QBH27_003800"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDQ7016061.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1577..2509)
                     /locus_tag="QBH27_003801"
     CDS             complement(1577..2509)
                     /locus_tag="QBH27_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7016062.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHRES"
     gene            complement(2601..3098)
                     /locus_tag="QBH27_003802"
     CDS             complement(2601..3098)
                     /locus_tag="QBH27_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDQ7016063.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3356..3961
                     /locus_tag="QBH27_003803"
     CDS             3356..3961
                     /locus_tag="QBH27_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDQ7016064.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLKLARERGFEEIAQLLIAAGA"
     gene            4001..4864
                     /locus_tag="QBH27_003804"
     CDS             4001..4864
                     /locus_tag="QBH27_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDQ7016065.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHDDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAV
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4854..6401
                     /gene="fdrA"
                     /locus_tag="QBH27_003805"
     CDS             4854..6401
                     /gene="fdrA"
                     /locus_tag="QBH27_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDQ7016066.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAISMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLDQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6401..7819
                     /locus_tag="QBH27_003806"
     CDS             6401..7819
                     /locus_tag="QBH27_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDQ7016067.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7972..8922
                     /locus_tag="QBH27_003807"
     CDS             7972..8922
                     /locus_tag="QBH27_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDQ7016068.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQDGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8932..10314
                     /locus_tag="QBH27_003808"
     CDS             8932..10314
                     /locus_tag="QBH27_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDQ7016069.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQTGFECEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10691..11740
                     /gene="yahK"
                     /locus_tag="QBH27_003809"
     CDS             10691..11740
                     /gene="yahK"
                     /locus_tag="QBH27_003809"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDQ7016070.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         2989 a         2885 c         3172 g         2727 t
ORIGIN      
        1 tgacgtgtat gcataaccaa catctttact cattatgtca ttgaatgttg acgctatgtg
       61 tttatgaggt tgaggtattt tcagttgatc tggattgtta aattcatata atgcgccttt
      121 actcatgaat ggacgtcaat acgtagtggg aaattataaa tatcgaaata gtccaactac
      181 ttctttatta ccaaaaatga gtatctgaat tttaatattg cattcttgcg tgattatctc
      241 ctgagtttga cttgtgatta cctttttaag gtatttagcg taactgtttt tgagcgagca
      301 tcagaggtaa agataatctt cttgatagtg atgtgggatg ttatacgtat ggcatcgcta
      361 atgtttatgg ttacccctta tgtgtgctca ggaatccaca ggtaatcact catactgaac
      421 agcgataaaa gataaaaggt gtgttcatga attcatgtga ttttcgtgtt tttctgcaag
      481 agttcggtac aacggttcat ttgtcattgc ctggtagcgt atccgagaaa gaacgactgc
      541 tactcaagct gctgatgcag ggaatgtctg taacagaaat atcacagtac agaaatcgca
      601 gtgcaaagac catttcacat caaaagaaac agctctttga gaaactgggg attcagagcg
      661 atattacttt ctggcgggat attttctttc agtacaatcc ggagatcata tccgccacgg
      721 gtaataatag tcacaaatat attaatgata atcactatca ccatatcgtc acgcctgaag
      781 ccatcagtct ggcgttggaa aaccacgagt ttaaaccgtg gatccaaccg gttttctgcg
      841 cgcagactgg ggtactgacg ggctgtgaag tgcttgtccg ctgggaacat ccacaaacgg
      901 gaatgatccc accggatcag tttattcctc tggcggagtc atccggtctt attgtcataa
      961 tgactcgcca gctgatgaaa cagactgcgg atattctgat gccggtaaaa catttgctgc
     1021 cggacaattt ccatattggt atcaacgtct cggcgggctg ttttttggct gcgggatttg
     1081 aaaaagagtg tctgaacctg gttaagaaat taggtaacga taaaatcaag ctggttcttg
     1141 agttgactga acgtaatcct attccggtaa cgccagaagc cagagcgata tttgacagcc
     1201 ttcatcagca caatattact tttgcgctgg atgactttgg tacgggttat gcgacctatc
     1261 gttacttgca ggcgttcccg gtcgatttta ttaaaattga caagtcgttt gtgcaaatgg
     1321 cgagtgtcga cgaaatctcc ggtcatattg tggacaatat tgtcgaactg gcgcgtaagc
     1381 ctggtttgag tatcgtggcg gaaggggtag aaacccagga gcaggcggat ttaatgatcg
     1441 gcaaaggcgt tcacttttta cagggctatt tgtactctcc gccagtaccg ggtaataaat
     1501 ttatctctga atgggtaatg aaagcaggtg gttgatgtaa accgctattc acagcgcatc
     1561 gggaggttgg cagcgattaa ctctcccgat gcagtaaatc ctgatagatc cccgttaata
     1621 tgccatttgg cgcaaattgt tttttaatcc actgcgtcac ctgaccggtg gcagagtgct
     1681 gggttgcgag taacatgcgt gaatcctggc gcgggttatg gatttgtcgg gtaaccagca
     1741 aggattgcgc catcgcctca cggaccatgt aatccggcaa aaaaccaatt ccttcaccta
     1801 aaatctggca ctgacatttg gtgttgaaat cgggcaccag aatcgattcc tgcccatgca
     1861 gcaaccagcc tactttttta ttaatcgtat gcgcggtgtc ctccaccatg atattagggt
     1921 agagacgcag ctggctttcg gcgatcggct ccggcacaaa ggctaatgga tgatccggag
     1981 cgatggcaaa agcccagcga atcgcgccaa tctccgtgta atcaatgccg ccaccgtcca
     2041 gcagcgtgtc gggtgcgccg atagcgatat ttgcctgatt attaataatc gcatcccaga
     2101 cgccgttata cacttcggtg gtgacggtaa tctggcaggt aggaaactgc tttttcagca
     2161 cctgtaataa gcgtgcggtg tgtttgggcg tataaagcag ctgattaata caaatgcgca
     2221 cacgcgcttc tatgccctgc gaaatagtat cgatactgcg tttaatggcg tgaaaatcat
     2281 tcagcaggtc ggtagctttg cggaaaaaat aacgcccgga ctccgttaac tcaatgctgc
     2341 gggtactgcg agtgaacagc accacatcca gccctgtttc cattcgcttg atggtgtagc
     2401 taatggcgga agtggttaaa cccaactctt ctgcggcttt actgaagctg ccaaaacgtg
     2461 cagcggtagt aaatgccaac agattctctt cggtaaaaat tgagttcatt aaaccattcc
     2521 cgccattatc gaatatgaat ttctccagcc atgactgtag aggtatcggt taaagataga
     2581 taatcatttt tgaataactt ttaatacccg tcgcgtttag tcgtcgcttc gcttaatttt
     2641 tgtcctccat attctgaaat tatccccacg cagttacccg ccagaaaacc cagtaatgtc
     2701 accgtccagt tcatcccgct ggcaaaaaat agcgtcatgc cgagaaagcc tccgggaata
     2761 aaagacaata aaccgaaacg tccttgccag accactaccg ctgagaaagg cacggtagta
     2821 atcacgctcg cccagaaccc agaaatccca ctcaccgagg ccagccaacc ggcagccagc
     2881 gcggcgaaaa atgcccacac catcccggaa taattaaccg ccagacttct gataaacccc
     2941 gatttaccgc cgcctgcggc ataaaaactg caaaaggcga cgaagccaat ggttcctaat
     3001 aattcccatc cctgagagag cccgacatgt gaagacacta actgccaaag ccctgcacaa
     3061 ataccgacgg tgacacccgt tgccgtgagt ccgttcatct tgttcccctc tggttagaca
     3121 tggacaaaag ctactcttac atgtgcagac tatagagaaa taaaacctga ttgagtgaaa
     3181 gggtatcggg tcaaagaaac aaatattgca ctaccgcaca ctgcgaaaag attgttgaac
     3241 atcattcaac aaaaaggcga aatacggcga attaaattta agcgggttct tttatcagat
     3301 tatcgttgct attctcaatt catcgaaaac agccacaaaa accaggggaa tgtgaatgac
     3361 tataaaaaat ctacctgccg actatttatt ggctgcgcaa cagggtgata tcgataaagt
     3421 aaaaacctgc cttgcgctgg gtgtcgatat taatacctgc gatcgtcagg ggaaaacggc
     3481 aattacgctg gcaagtttat atcagcaata tgcttgcgtt caggcattaa ttgatgccgg
     3541 agcggatatt aataagcaag atcatacctg tttaaatcct tttttaatta gctgtctgaa
     3601 cgatgattta acgctactac gaattatttt accggctaaa cccgatctta attgcgtaac
     3661 ccgttttggc ggtgtcggcc tgacgcctgc ctgtgaaaaa ggccatttaa gtattgtaaa
     3721 agagcttttg gcaaatacgg agattaacgt taaccagact aaccatgttg gctggacacc
     3781 gttgctggaa gcgattgtgc ttaatgatgg tgggattaaa cagcaggcga ttgtccagtt
     3841 attgctggaa cacggtgcca gcccgcatct gaccgataaa tatggcaaaa cgccgctgaa
     3901 actggcgcgg gaacggggct ttgaagagat tgcgcagttg ctgatcgccg caggtgcata
     3961 aaccgggagg cttgttatca acacaccaga aaaacggtgt gtgtgggcgc taactgcgga
     4021 tgctgatttt ctggcgcagc gggggcaagg acaggttgaa caggtctttg ccagagcggt
     4081 aaatatcgca ctcccggctc gccagcagtt gctgacgctg ctttgtgaag agtacgacaa
     4141 tgcgcctaac agttgtcggt tggcactcac tcactttaat ggtctgttcc ggcatgatga
     4201 taaggttcag tttgacgatc aaggcattac gattggtcaa catcatcata tagagatgag
     4261 tcattgccag cgttggctgt ccccaacctt gcaaatgacc gctgtgaatt ttcaccttat
     4321 cgcctggcaa cagtggtacg acattattca tcagcacctg ggggaaaatg aaaccctgtt
     4381 taattatcgc ggcgataatc cgttttatca ggcgttaaat aaagaactac atattaaacg
     4441 acgggcagtt attcaggccg taaacgataa acaaaatatc gcctcagcag tcgccagtat
     4501 gatggggtta gggattggcc ttacgccatc agccgatgat tatctgaccg gactggtact
     4561 tattttattt atttccggac atccggcgga aaaatacaaa gaggaatttt atctcggcct
     4621 gcaacgcggc agaaataata ccacgttatt aagtgccatc acgctggaag ccgcattaca
     4681 acaacgctgc cgggaaaata ttcatcgttt tattcacaac attatttatg acatccctgg
     4741 gaacgcaact caggcaatag aaaaaattaa acatattggc tccagttccg gctgcgacat
     4801 gctgtatggc atggccgatg gttgtgcgct gagtcaaacc tacggaggga attatgtcag
     4861 ttaaaatagt cattaaaccg aatacctatt ttgattctgt ctcgctgatg tctatctcca
     4921 cgcgtgcaaa taaactcgac ggcgtcgagc aggcatttgt ggcgatggcg accgaaatga
     4981 acaaaggcgt gttgaagaat ttaggactgc tgacgccgga gctggagcag gcgaaaaacg
     5041 gcgacctgat gattgtcatc aatggtaaat cgggtgtgga caacgagcag ttgctggcgg
     5101 agattgaaga actgttcaac accaaagcgc aaagcggctc gcacgaggcg cgttacggca
     5161 ctattgccag cgccaaaaag catatcccgg aaagtaacct ggcagtgatt tcggtcaacg
     5221 gtctgtttgc cgctcgcgaa gcgcgtcagg cgctgcaaaa tgacctcaac gtgatgctgt
     5281 tttccgataa tgtctcagtt gaagatgaac tggcgctcaa acagttggcc catgaaaaag
     5341 ggctgctgat gatggggcca gactgcggca cggcgattat caacggcgcg gcgctctgct
     5401 ttggtaacgc cgtgcgtcgc ggcaacatcg gtattgttgg cgcatctggc accggcagcc
     5461 aggaattgag cgtccgtatc catgaatttg gtggcggcgt ttcacaactg atcggcaccg
     5521 gcgggcgcga cctgagcgag aaaatcggcg gactgatgat gctcgatgcc atcagcatgc
     5581 tggaaaacga tccgcaaact gaaatcattg tgcttatctc caaaccgcct gcgcctgcgg
     5641 tggcccgcaa agtgctggaa cgcgcacgcg cctgccgcaa gccagtggtg gcctgcttcc
     5701 tcggtcgtgg cgaaacgcca gtggatgagc aggggctaca gtttgctcgc ggcagcaaag
     5761 aggcggcatt gaaagcggtg atgctctccg gcgtgaaaca agaacatctc gatctgcata
     5821 cgcttgacca gccgttgatt gcggatgtgc gcgcgcgtct gcaaccgcag cagaaataca
     5881 ttcgtggtct gttctgcggc ggtacgctgt gtgacgaaac cctgttcgcg gtgatggaaa
     5941 aacatggcga tgtctacagc aatatccagc ccgatcctga attccgcctg caagatatca
     6001 accgcagcat caaacacacc ttcctcgact ttggcgatga tgacttcacc aacggcaagc
     6061 cgcatccaat gattgacccg accaaccgta tcagccgctt gatcgaagaa gcgcgcgatc
     6121 cagaagtggc ggtgatcgtg atggattttg tgctcggatt tggatcgcat gaagatccgg
     6181 tcggttccac catcgaggcg atcaaagaag cgaaagcgat cgccgctgcc gaaggacgcg
     6241 agttgatcat tctcgcctat gtgctgggca ccgatctcga tacgccatca ttagaacaac
     6301 aaagccagat gctgcttgat gccggagtga ttctggcgag cagcagcacc aataccggat
     6361 tgctggcgcg tgaatttatc tgcaaagggg aggaagcctg atgagccagt cactgtttag
     6421 ccaaccgttg aacgttatta acgtcggtat cgccatgttt agcgatgatc tgaaaaagca
     6481 gcatgtagaa gtgacccaac tcgactggac gccgccgggg cagggcaata tgcaggtggt
     6541 gcaggcactg gacaacattg ccgattcgcc gctggcggac aaaatcaccg ccgctaacca
     6601 gcaggcgctg gagcgcatta tccagtcgca tccagtgctg attggttttg atcaggcgat
     6661 taacgtggtg ccgggcatga cggcgaaaac cattcttcac gccgggccgc cggtcacctg
     6721 ggaaaaaatg tgcggcgcga tgaaaggcgc ggtcaccgga gcgctggtgt ttgaaggact
     6781 ggcgaaagat ctcgacgagg cggctgagct ggcggcttcc ggggagatca ccttctcgcc
     6841 gtgccacgag cacgactgtg tgggatcgat ggcgggcgtc acctcggcct cgatgtttat
     6901 gcacatcgtg aaaaacaaaa cctatggcaa catcgcttat accaacatga gcgagcagat
     6961 ggcgaagatt ttgcgtatgg gcgctaacga ccagagcgtg atcgaccgcc tcaactggat
     7021 gcgcgatgtg caggggccaa tgctgcgtga cgcgatgaaa attatcggcg agatcgatct
     7081 gcgcttaatg ctggcgcagg cgctgcatat gggcgatgaa tgccataacc gcaataacgc
     7141 cgggacgacg ctgctgattc aggcgctgac gccggggatt attcaggcgg gttattccgt
     7201 tgagcaacag cgcgaagtgt ttgagtttgt cgccagcagc gattacttct ccggcccaac
     7261 gtggatggcg atgtgtaaag cggcgatgga tgcggcgcac ggcattgaat acagcaccgt
     7321 ggtgaccacc atggcgcgta acggcgtcga gttcggcctg cgggtcagcg gcctgccggg
     7381 gcaatggttt accggcccgg cgcagcaggt catcggcccg atgtttgccg gttataagcc
     7441 ggaagattcg gggctggata tcggcgacag cgccatcacc gaaacctacg gtatcggcgg
     7501 atttgcgatg gcgaccgcgc ccgcgatcgt tgcgctggtg ggcggcacgg tggaagaggc
     7561 tatcgatttc tcccgtcaga tgcgtgaaat cacccttggc gaaaacccca acgtcaccat
     7621 tccgctgctc ggttttatgg gcgtgccgtc ggcaatcgac atcacccgcg tgggtagcag
     7681 cggcattctg ccggtgatca acaccgccat tgcccataaa gatgcgggcg tcggcatgat
     7741 tggcgcgggc attgtgcatc cacccttcgc ctgcttcgag aaagccattc ttggctggtg
     7801 cgaacgttac ggcgtctgac ctgcaaagta cgcgttgtct ctgccggatg cggcgtgaac
     7861 gccttatccg gcctacgagt ggcacgagaa ttgtaggcct gataagcgta gcgcatcagg
     7921 cagtctggcg ttggtcataa ccccatcacc ctctgtcgcg gacataacaa catgaaagaa
     7981 cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag ccagtcgatt
     8041 gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat gctggcttcc
     8101 gattacgaca ttgtgctgac ccacggcaac gggccgcagg tcgggctgga tttacgccgt
     8161 gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa ctgtgtggcg
     8221 gatacgcaag gtggcattgg ctatctgatc cagcaggcgc tgaacaaccg actggcgcgt
     8281 cacggcgaga aaaaagccgt cactgtggtg actcaggtgg aagtggataa aaacgatccg
     8341 ggttttgccc atcccaccaa gcccatcggc gcattcttta gtgaaagcca gcgtgacgaa
     8401 ctacaaaagg caaaccctga ctggcgtttt gttgaagatg ccggacgggg ctatcgccgc
     8461 gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ctgccattaa ggcgctgatc
     8521 caacaaggct ttgtggtgat tggcgcgggc ggcggtggaa ttcctgtggt gcgcaccgaa
     8581 gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctccac cgcgctactg
     8641 gcccgcgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa agtctgtatc
     8701 cactttggca agccgcagca acaggcgctc gatcgcgtag atattgccac catgacccgc
     8761 tatatgcagg aagggcattt cccacctggc agcatgttgc caaaaatcat cgccagcctg
     8821 acgttcctgg aacaggacgg caaagaagtg attatcacca cgccggaatg cctgcctgcg
     8881 gcgctacgcg gcgaaacggg cacccatatt attaaaacct aaggacgtaa gatgaaagaa
     8941 aacaatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc cgccgcgctg
     9001 tttggtacct cagtgccgct cgcccatgcg ggggtagctg gcaccctaaa ctgcgaagcg
     9061 aacaacacca tgaaaatcac tgacccgcat tactatctcg ataacgtgct gctggaaacc
     9121 ggttttgact acgaaaatgg cgtggcggta cagacccgca cggcgcgcca gaccgtggag
     9181 attcaggacg gtaaaattgt tgccctgcgc gagaacaagc agcatccgga cgccacgctg
     9241 ccgcactatg acgctggcgg taagctgatg ctgcccacca cccgcgatat gcatattcat
     9301 ctcgacaaaa ccttctacgg cgggccgtgg cgctcgctca atcgtccggc aggcaccact
     9361 atccaggaca tgatcaaact cgagcagaaa atgctgccgg aactgcaacc gtacacgcag
     9421 gaacgggcgg aaaaactgat cgatttattg cagtcgaaag gcaccactat tgcccgcagc
     9481 cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca ggcggtgctg
     9541 gcgcgacgtc agacgggctt cgagtgtgag atcgtcgcct tcccgcagca cggtttgctg
     9601 ctgtcgaaat cggaatcctt aatgcgcgaa gcgatgcagg cgggggcgca ttacgtcggc
     9661 gggctggacc cgaccagtgt tgatggcgcg atggaaaaat ccctcgacac catgttccag
     9721 attgcgctgg actatgacaa aggcgtcgat attcacctgc acgaaaccac gccggcaggc
     9781 gtggcagcca tcaattatat ggttgaaaca gtggagaaaa cgccacagct gaagggcaag
     9841 ctgaccatca gccacgcctt tgcgctggca acgctcaacg aacaacaggt agatgaactg
     9901 gcgcaccgga tggcggcgca gcgaatttcg atcgcttcga cggtgccgat tggcacgctg
     9961 catatgccgc tcaaacagct gcacgacaaa ggcgtaaaag tgatgaccgg caccgacagc
    10021 gttatcgacc actggtcgcc ttacggcctg ggcgacatgc tggaaaaagc caatctgtac
    10081 gcgcagctct atattcgtcc taacgaacag aacctctccc gctctctgtt tttagccact
    10141 ggcgatgtat tgccgctcaa cgaaaaaggc gagcgcgtgt ggcccaaagc gcaggatgat
    10201 gccagttttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg tatttcgccg
    10261 agaacggcga cgttccataa agggcaactg gtgtggggga gtgtggcagg ttgatacgaa
    10321 cttgatatgt ttgcaacggt gaacagtaag agatttaagc cccagggagt ggggcggcct
    10381 gagtatgagt gtgtcggcag aaatggacat tatctttgaa gaatatgccc ttattgacgt
    10441 tgtgtagata actatttgtc tacgtgaaaa tatcatcagt tttgccgatt tttgtctttt
    10501 acggatagca aatatcacac ttataggcgc tcgccttatg gggaacgttg ctgctctgtt
    10561 tcattcaggc tcgcggcatg ttagggctgg atcgggtggt ttaaatattg gctatattca
    10621 atggacgcgt tttgccgcga tgacatatca ggcatcgtca aatacacata gctaatcagg
    10681 agtaaacaca atgaagatca aagctgttgg tgcatattcc gctaaacaac cgcttgaacc
    10741 gatggatatc acccggcgtg aaccgggacc gcatgatgtc aaaatcgaaa tcgcttactg
    10801 tggcgtctgc cattccgatc tccaccaggt ccgttcggag tgggcgggga cggtttaccc
    10861 ctgcgtgccg ggtcatgaaa ttgtggggcg cgtggtagcc gttggtgatc aggtagaaaa
    10921 acatgcgccg ggcgatctgg tcggtgtcgg ctgcattgtc gacagttgta aacattgcga
    10981 agagtgtgaa gacgggctgg aaaactactg tgatcacatg accggaacct ataactcgcc
    11041 gacaccggat gaaccgggcc atactctggg aggctactca caacagatcg tcgttcatga
    11101 gcgatatgtt ctgcgtattc gtcacccgca agagcagctg gcggcggtgg ctcctttatt
    11161 gtgtgcagga atcaccacgt actcgccgct acgccactgg caggccgggc cgggtaaaaa
    11221 agtgggcgtg gtcggcatcg gcggtctggg acatatgggg attaaactgg cccacgcgat
    11281 gggggcgcat gtggtggcat ttaccacttc tgagtcaaaa cgcgaagcgg caaaagccct
    11341 gggggccgat gaagttgtta actcacgcaa tgccgatgag atggtggctc atgtgaagag
    11401 tttcgatttc attttgaata cagtagctgc gccacataat ctcgacgatt ttaccacctt
    11461 gctgaagcgc gatggcacca taacgctggt tggtgcgcct gcgacaccgc ataaatcgcc
    11521 ggaagttttc aacctgatca tgaaacgccg cgcgatagct ggttctatga ttggcggcat
    11581 tccagaaact caggagatgc tcgatttttg cgccgaacat ggcatcgtgg ctgatataga
    11641 gatgattcgg gccgatcaaa ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa
    11701 atatcgtttt gttatcgata atcgcacact aacagactga aaaaaattaa taaatatcct
    11761 gtggtttaac ata
//
LOCUS       DAOFSV010000138        11414 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.138, whole genome shotgun sequence.
ACCESSION   DAOFSV010000138 DAOFSV010000000
VERSION     DAOFSV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.138"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(19..678)
                     /gene="rluA"
                     /locus_tag="QBH27_003810"
     CDS             complement(19..678)
                     /gene="rluA"
                     /locus_tag="QBH27_003810"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDQ7016071.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(690..3596)
                     /gene="rapA"
                     /locus_tag="QBH27_003811"
     CDS             complement(690..3596)
                     /gene="rapA"
                     /locus_tag="QBH27_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDQ7016072.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(3761..6112)
                     /gene="polB"
                     /locus_tag="QBH27_003812"
     CDS             complement(3761..6112)
                     /gene="polB"
                     /locus_tag="QBH27_003812"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDQ7016073.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6187..6882)
                     /gene="araD"
                     /locus_tag="QBH27_003813"
     CDS             complement(6187..6882)
                     /gene="araD"
                     /locus_tag="QBH27_003813"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDQ7016074.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(6997..8499)
                     /gene="araA"
                     /locus_tag="QBH27_003814"
     CDS             complement(6997..8499)
                     /gene="araA"
                     /locus_tag="QBH27_003814"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDQ7016075.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(8510..10210)
                     /gene="araB"
                     /locus_tag="QBH27_003815"
     CDS             complement(8510..10210)
                     /gene="araB"
                     /locus_tag="QBH27_003815"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDQ7016076.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELCVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRNIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEVWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            10549..11394
                     /gene="araC"
                     /locus_tag="QBH27_003816"
     CDS             10549..11394
                     /gene="araC"
                     /locus_tag="QBH27_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDQ7016077.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGIVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWVNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT         2522 a         3161 c         3093 g         2638 t
ORIGIN      
        1 acttcgccag cgtgactatt aaaaatccgc tggtgcttta aacgtcatac tattgccata
       61 cgccggatgg gtaatcgtca gcatctctgc atgtagcaac aaacgcggtg ccattgctct
      121 cgcttctggt gatgcataaa aacgatcgcc gagaatcgga tgacccagcg ccagcatatg
      181 cacacgcagc tgatgcgaac gcccggtaat cggttttaac accactcttg ccgtgttatc
      241 cgccgcatat tccaccactt catactccgt ctgcgcaggt ttacctgttt cgtaacagac
      301 tttctgtttc gggcggtttg gccagtcgca aatcagcggc agatccacca gaccttccgc
      361 aggggatgga tgcccccaga cgcgggccac atactgtttt ttcggctcgc gctcgcggaa
      421 ctggcgtttt aactcccgct ccgcggcttt ggtcagcgcc actacaatca cgccgctggt
      481 agccatatcc agacgatgca ccgattctgc ctgcggataa tcacgctgaa tgcgcgtcat
      541 cacgctgtct ttgtgctctt ccagacgacc cggcacagac aacaaaccgc tcggcttgtt
      601 gaccaccata atatggtcat cctgatacag gacaaccagc cagggttcct gcggcggatt
      661 gtagttttcc atccccatct tcggctccgt tactgatgcg tcacaacgat caaacgcagg
      721 gcatccagac gccagcctgc ctgatccagg ctttccatta cctgctgacg gttgctctca
      781 atggcggtca gttcgtcgtc acgaatgttc gggttaactg cacgcagcgc ttccagacga
      841 gacagctcgg cagacagttt ttcgtcggct tcgttacgcg ctgcatcaat caatgcacgg
      901 gcagatttct cgatctgcgc ttcccccagc tgaaggatag cgtgaacatc ctgctgcacc
      961 gcgttaacca gcttgctgcc ggtgtgacgg ttaaccgcgt taagctgacg gttaaaggtt
     1021 tcaaattcta cctgcgccgc caggttgttg ccgtttttat ccagcagcat acgtaccggc
     1081 gtcggtggca ggaagcggtt gagctgcaac tgcttcggag cctgggcttc aaccacataa
     1141 atcagttcca ccaacagcgt acctaccggc aacgctttgt ttttcaacag tgaaatcgtg
     1201 ctgctaccgg tatcgccaga aaggatcaga tccagaccgt tgcggatcag cggatgctcc
     1261 caggtaataa actgtgcatc ttcacgcgcc agcgccactt cacggtcgaa ggtgatggtg
     1321 atgccatctt ccgacaggcc agggaagtcc ggcaccagca tatgatcgga cggcgtcagc
     1381 acgatcatgt tgtcgccgcg atcgtcctga ttgataccga taatatcgaa caggttcatg
     1441 gcgaaggcga tcagattggt atcgtcatcc tgctcttcaa tgctttctgc cagtgcctgg
     1501 gctttttcgc caccgttgga atggatctcc agcaggcggt cacgcccctg ttccagttgt
     1561 gctttcagcg cttcgtgctg ctcgcggcag tttttgatta gatcgtcaaa gccttcggtc
     1621 tcatccggtg aagccagata gttaatcaga tcgttgtata cgctatcgta aatagtgcgt
     1681 ccggtcgggc aggtgtgctc gaacgcatcc agaccttcgt gataccagcg caccagcacc
     1741 gactgagccg ttttttccag ataaggcaca tggatctgaa tatcgtgcgc ctgaccgata
     1801 cgatccagac gcccaatacg ctgctccagc agatccgggt tgaatggcag gtcaaacatc
     1861 accatgtggc tggcgaactg gaagttacgt ccttcagaac cgatttccga gcacagcagt
     1921 acctgcgcac cggtgtcttc ttcagcaaac caggcggcag cgcggtcacg ttcgataatc
     1981 gacatgcctt cgtggaacac tgcggcgcga ataccttcac gttcgcgcag tacctgctcc
     2041 agttgcagcg cagtggccgc tttggcgcag atcaccagca ctttctgaga gcgatgactg
     2101 gtcaggtagc ccatcagcca ttcaacgcgc ggatcgaagt tccaccaggt ggcgttatcg
     2161 ccttcaaatt cctgataaat acgctccgga tagagcatat cgcgagcgcg atcttccgca
     2221 cttttgcgtg cgcccataat gccggagact ttaatagcgg tctgatactg cgtcggcagt
     2281 ggcagcttaa tagtatgcag ctcgcgtttc gggaagcctt tcacaccgtt acgggtgtta
     2341 cggaacagca cgcggctggt accgtggcga tccatcagca tcgaaaccag ttcctgacgg
     2401 gcgctctggg catcttcgct gtcgctgttt gctgcttgca acagcggctc gatatcctgc
     2461 tcgccgatca tctcaccgag catgttcagt tcgtcattgc tgagtttgtt acccgccagc
     2521 agcatggcaa cggcgtccgc aaccggacga taatttttct gctcttcaac gaattgcgca
     2581 aaatcgtgga aacggttcgg gtccagcaga cgcagacggg cgaagtggct ttccatcccc
     2641 agctgttccg gggttgcggt cagcagcaga acgcccggca cgtgctctgc cagttgttca
     2701 atggcctgat attcacggct cggcgcatct tcgctccaca ccaggtgatg cgcttcatcg
     2761 accaccagta gatcccactc ggcttcacag agatgttcca gacgctgttt gctacgacgg
     2821 gcaaaatcca gcgagcaaat caccagctgc tcggtatcga acgggttgta agcatcgtgc
     2881 tgagcttcgg cataacgctc atcatcaaac agagcaaagc gcaggttgaa acggcgcagc
     2941 atttctacca gccactgatg ctgtaaggtt tccgggacga taatcagcac acgttcagca
     3001 gcgccagaga gcagttgctg atgcaggatc atcccggcct caatggtttt ccctaaaccc
     3061 acttcgtcag ccaacaggac gcgcggcgcg tggcggcgac caacatcatg agcgatgttg
     3121 agctggtgcg ggatcaggct ggtacgctga ccgcgcaggc cgctgtacgg catacggaac
     3181 tgttcgctgg aatacttacg cgcgcgataa cgcagcgcaa agcggtccat acggtcaatc
     3241 tgcccggcaa acagacggtc ctgcggtttg ctgaacacca gtttgctatc aaggaaaact
     3301 tcacgcaggg ccacgccgga ctcttcagta tcaaggcgag taccgatata ggtcagcaag
     3361 ccattttctt cttttacttc ttcgacttgc atctgccagc cgtcatggct ggtaatggta
     3421 tcaccagggt tgaacatcac gcgggtcacg ggggaatcac tgcgtgcgta cagacggttt
     3481 tcaccagtag atgggaaaag taaagtgaca gttcgcgcat ccaccgcgac aacggttcca
     3541 agtcccaatt cgctttctgt atcgctgatc cagcgttgac caagtgtaaa aggcatatgt
     3601 gttcggctct atatctttaa ttgcaggcaa taaccacccg ctaccgtgct tatgaggtag
     3661 tggtgttatt caggtccagg aatggaaagg gcgctatggt actggatggc aaagcattcg
     3721 tcacgcatca aaatggtatc tggcgaactc ttttttttgc tcaaaatagc ccaagttgcc
     3781 cggtcataag tgtagcaaaa ttatcctcaa taaaagggag tattccctcc gctacgggtt
     3841 gtagctggcg ggtcagatag tgttcgtaat ccagtggtga acgttggtag tccagcggct
     3901 ccgggccgtt ggtggtccat acgtacttaa tggtgccgcg gttctgatat tgcaaggggc
     3961 gaccgcgctt atggttttct tcatcggcaa ggcgagcggc gcgtacatga ggcggcacat
     4021 tacgctgata ttcgctcagc ggacggcgaa ggcgtttacg gtaaaccagc cgcgcatcca
     4081 gttcacccgc catcagtttg tcgatggttt cacgcacgta ttcctgatat ggctcgttgc
     4141 ggaagatacg caggtaaagc tcctgctgaa actgctgggc cagtggcgtc cagtcggtgc
     4201 gcacggtttc cagcccttta aacaccatcc gctgtttgtc gccctcctga atcagtccgg
     4261 cgtagcgctt tttgctgccg gtatcggccc cgcgaatggt tggcatcaga aaacggcaga
     4321 aatgggtttc atactccagt tccagtgcgc tggtcagccg ttgtttttgc agcgtttccg
     4381 cccaccaggc gttaacgtgc tgcaccagtg cacgaccgat tttcgctgct tcttcttccg
     4441 aatgtgcacc tttcagccag acaaacgttg agtcggtatc gccgtagata acgtcatagc
     4501 cctgcgcttc aatcaacgtt ttggtttgcc gcatgatctg atgaccacgc atggtgatcg
     4561 acgatgccag gcgtggatcg aagaaacggc aggcggtggt gccgagcaca ccataaaagg
     4621 cattcataat gatcttcagc gcctgcgaaa gcggcttatt accctgacgt ttggcttcat
     4681 cgcgcccgtg ccagatgtta gtcacaatct ccggcaggca atgtttctcc cgtgagaacc
     4741 acgcatcgag aaaaccttcg gtactgtgct ctggatcagg ctgcgccatg ccttccacca
     4801 gcccgacggg atcaatcaga aaggtgcgga tgatcgacgg gtacaggctt ttgtagtcca
     4861 gcaccagcac cgaatcataa agcccaggtc gcgaatccat cacgtagccg ccagggctgg
     4921 cgtgcggcgg cacttcgccg agattaggcg cgacataacc agcgcgatgc atacggggaa
     4981 aatagagatg accaaacgct gccaccgaac cgccgtgtcg atccaccggc aggccgttca
     5041 ccgtcgcccg ttcgagcaaa aacggcataa tttcagtttt gtggaagatc tgcgtcacca
     5101 gctcgcaatc tttcaggtta taagttgcca gcgcaggttt atcttcggcg aaacggcggt
     5161 caatttcgtc cattcgatcc cacgggttat cgatagattt tccttcgcct aataactcct
     5221 gagcgacggt ttccagcgag aatgaagaga aattccagaa cgcggatttc agcgcctcga
     5281 taccgtcgat aattagccga cctttaacct gagcaaaaaa cacaccgttt ttaaagccgt
     5341 gctcgcgcca ctccagctca ctgttatcac gccccagccg cagcggaata cggtaacgct
     5401 cggcatgttt ttgcagcatt cgcagatcga actgcaccac gttccaaccg atgatcacat
     5461 caggatcgta gttggcaaac caggcgttaa gtttttccag tagcagcggg cggctggcga
     5521 cgtattccag ttcaaaatcg agcgcggagg cgtcgccatt ctctggcccc agcatataaa
     5581 cgatacgctg cccgcaacct tccaggccga tgcagtacag ctcaccgtgg cgggtggttt
     5641 caatatcaat agaaacccac ttgagcggcg gacgatagtc gggatgcggt ttcagacggg
     5701 cattaacgat agcgccattg cgcatatcac cctcgaccca caccggtgag gtgataaatc
     5761 gctccatcag atagcgttct ggcgggcgca catcggcctc gtagacggta acgccacctt
     5821 cacgcaggcg cttttcgtaa ttcatcaact ggcgatgggc gcgacagtaa aggccataca
     5881 ccggctggcg gtgaaaatct tttaacgcca gcggcgtcag gcgaaagcct tgttcaccct
     5941 gcaaaatatg ctgagcgcgg ggaacctgat cggcaggaat aaacgccacg gactcttgcg
     6001 gcgcaaggaa aacctgcaac ggcccgttgt ccgtcgccag ccagaaggaa acttcggtcc
     6061 cttgcggggt gtcccgccag tgtcgggtta agataaaacc tgcctgcgcc acgctgaaaa
     6121 tccaataaaa aaccaggctt gattatagcc tggtttcgtt tgattggctg tggttttata
     6181 cagtcattac tgcccgtaat atgccttcgc gccatgctta cgcagatagt gtttatccag
     6241 cagcgtttgc tgcatatccg gtaactgcgg cgctaactga cggcagaata tacccatata
     6301 agcgacctct tccagcacga tggcattatg caccgcatct tcggcatttt tgccccatgc
     6361 aaatgggccg tgagaatgga ccaggacgcc gggcatttgc gctgcatcga taccctgttt
     6421 ttcgaaggtt tctacgatga cgttaccggt ttcccactca tattcaccgt tgatttctgc
     6481 gtcggtcatt ttgcgggtgc agggaatggg accgtagaaa tagtcggcgt gggtggtacc
     6541 ggttgccgga attgactgac ccgcctgcgc ccagatggtg gcgtggcgcg agtgtgtgtg
     6601 cacaatgccg ccaatggacg ggaatgcctg atagagcagc cggtgagttg gcgtgtcgga
     6661 ggagggcttt ttcgtacctt caaccacttc accggtttcg atgctgacca cgaccatatc
     6721 gtcagcggtc atgatgctgt agtcgacgcc ggaaggtttg atcacaaaga cgccgcgccc
     6781 gcgatcaacg gcgctgacgt tgccccaggt gagcgtgacc aggttgtgtt ttggcagcgc
     6841 caggttggct tctaatacct ggcgtttgag atcttctaac atgttgactc cttcgtgccg
     6901 gatgcgattt gcttatccgg cctacaaaat cgcagcgtgt aggcctgata agacgcgtcg
     6961 gcgtcgcatc aggcgttaca tacctgatgc ggctacttag cgacgaaatc cgtaatacac
     7021 ttcgttccag cgcagcgcgt ctttaaacgc tggaaggcgg gtatcgttat caatcaccgt
     7081 gatttcaatg tcgtgcatct cggcgaactg gcgcatatcg ttgaggttca gcgcatggct
     7141 gaagacggta tggtgcgcgc caccggcaag gatccacgct tcggaagcgg tcggcagatc
     7201 cggttgcgct ttccacagcg cattcgccac cggcagtttc ggcagggagt gcggtgtttt
     7261 caccgtgtcg atgcagttaa ccagcagacg ataacgatcg ccgagatcaa tcaggctggc
     7321 gacaatggcc ggaccagttt gagtattgaa gatcaggcga gccgggtcgt ctttaccgcc
     7381 aataccaagg tgctgaacgt cgaggatcgg tttctcttcc acagcgatcg acgggcagac
     7441 ttccagcata tgggagccga gtaccaggtc attacctttc tcgaagtgat aggtgtagtc
     7501 ctccataaag gaggtgccgc cctgcagacc ggttgacatc accttcatga tgcgaagcag
     7561 ggcggcagtt ttccagtcgc cttcgcccgc aaagccgtag ccctgctgca tcagacgctg
     7621 tacggccaga ccaggaagct gtttcaggcc gtgcaaatct tcaaaggtag tagtgaaagc
     7681 gtggaagcca ccctgttcca ggaaacgctt catccccagc tcaatacgcg ccgcttccag
     7741 cacgttctgt cgtttttcgc cgtggatttg tgtggcaggt gtcatggtgt agcagctttc
     7801 gtactcatcg accagcgcgt taacatcgcc gtcgctgatg gagttcacca cctgcaccag
     7861 atcgccaacc gcccaggtat tgacggagaa accaaacttg atctgtgcgg caactttatc
     7921 gccatcggtg accgctactt cacgcatgtt atcgccaaaa cggcagactt tcagatgacg
     7981 ggtatcctgt ttagagaccg cctgacgcat ccaggagccg atacgctcat gtgcttgttt
     8041 atcctgccag tgaccggtaa caacggcatg ttgctgacgc atacgcgcgc cgatgaagcc
     8101 gaactcgcga ccgccatgcg cggtctggtt caggttcata aagtccatgt cgatgctgtc
     8161 ccacggcagc gccgcgttga actgggtgtg gaattgcagc aacggtttgt tgagcatggt
     8221 caggccgttg atccacattt tggccgggga gaaagtgtgc agccacacca ccagaccagc
     8281 gcaacgatcg tcgtaattcg cgtcgcggca aatagcggtg atttcatccg gcgtggtgcc
     8341 cagcggtttc agcaccagtt tgcagggcaa cttcgcttct gtattcagtg cattaacaac
     8401 gtgttccgca tgttgcgtca cctggcgcag agtctccggg ccgtaaagat gctggctgcc
     8461 aattacaaac cacacttcat aattatcaaa aatcgtcatt gtcgtgtcct tatagagtcg
     8521 gaacggcctg ggcagcctgt gccggggcgg aagttggaag atagtgttgt tcggcgctca
     8581 tcgcccattg ctgatagcgg cgataaagct gttcaaagcg ttgtgcctgt tcgctgcgcg
     8641 gttgcagggt tttctctacc gcactggcca ttttttgctg ggctgatggg atgtctgcgt
     8701 gcactttcgc ggcgacagca gcaaaaatcg ccgcaccgag cgcacagcac tggtcagagg
     8761 caacaatttg cagcgggcga ttcagcacgt cgcagcaggc ctgcataatg acctggtttt
     8821 tccgcgcgat gccgcccagc gccatcacgt tattgacggc gatcccctga tcggtaaagc
     8881 actccatgat tgcgcgtgcg ccaaaggcgg tggcagcaat caaaccgccg aacagcagcg
     8941 gagcgtcggt agcgaggttc agatcggtaa tcaccccttt caggcgttgg ttagcgttcg
     9001 gtgtgcggcg gccgttaaac cagtcgagca ccaccggtag gtgatccaga gacggatttt
     9061 gagcccatac ttcggtcagc gccggaagca gttgtttctg gctggcgttg atttgctctt
     9121 tcagttccgg atgctgggcg gcaagctgtt ccagcggcca gccgagtacg cgaccaaacc
     9181 aggcgtagat atccccaaac gccgattggc ctgcttccag accgataaat ccaggcacca
     9241 cgctgccatc aacctgaccg caaatacctt tcactgcccg ctcgccaacg ctctgtttgt
     9301 cggcaatcag aatgtcacag gtggaagtac cgataacttt taccagtgcg ttaggctgtg
     9361 cgcctgcgcc aactgcgccc atatggcagt caaacgcgcc gccggaaatc actacgcttt
     9421 caggcaggcc gagacgctgc gcccattccg ggcataaggt gcccaccgga atatcggcag
     9481 tccaggtatc agtgaacagc ggggaaggca aatggcgatt gaggatcggg tccagctcat
     9541 caaagaaact ggctggcggc aggccacccc agctttcgtg ccacagagat ttatgcccgg
     9601 cgctgcaacg tccgcgacga atatcctgcg ggcgggtggt accggaaagc agagctggca
     9661 cccagtcgca cagctcaatc catgatgcgg cagattgtgc cacggcgttg tcctggcgag
     9721 ttacatgcag gatttttgcc cagaaccatt cgctggaata aataccgcca atattgcggg
     9781 agtagtcaac gttgcccggc gtgtgacata agcgggtaat ctcttccgct tcttcaaccg
     9841 cggtgtggtc tttccacaat acgaacatcg cgttcgggtt ctcggcaaac tccgggcgca
     9901 gcgccaacac gttgccgtcg gcgtcaatcg gtgcgggcgt cgagccggtt gtgtcaacgc
     9961 caatcccgac cacagctgcg cgctgttcga cgcaaagctc tgcaagcaca gttttcagcg
    10021 ccgcttccat tgactcaatg tagtcacgcg gatgatgacg gaactggtta ttcggggcat
    10081 cacaaaattg cccgttctgc caacggggat accactctac gctggtggcg atctcttcac
    10141 cagtggcgca ttccaccgcc aaagctcgca cagaatcact gccaaaatcg aggccaattg
    10201 caatcgccat cgtttcactc catccaaaaa aacgggtatg gagaaacagt agagagttgc
    10261 gaaaaaaaac gtcaggctgg atccgctaat cttatggata aaaatgctat ggcatagcaa
    10321 agtgtgacgc cgtgcaaata atcaatgtgg acttttctgc cgtgattata gacactttta
    10381 ttacgcgttt ttgtcatggc tttggtcccg ctttgttaca gaatgctttt aataagaggg
    10441 gttaccggtt gggtcagcga gaagagccag taaaagacgc agtgacggca atgtctgatg
    10501 caatatggac aattggtttc ttctctgaat ggcgggagta tgaaaagtat ggctgaagcg
    10561 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg
    10621 ccgattgagg ccaacggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt
    10681 tatattctca atctcaccat tcgcggtcag ggcatagtga aaaaccaggg acgagaattt
    10741 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt
    10801 catccggagg ctcacgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg
    10861 catgaatggg ttaactggcc gtcgatattt gccaataccg gtttctttcg cccggatgaa
    10921 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaatgccgg acaaggggaa
    10981 gggcgctatt cggaattgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg
    11041 gaagcgatta acgagtcgct ccatccgccg atggataatc gggtacgcga ggcttgtcag
    11101 tacatcagcg atcacctggc agacagtaat tttgatatcg ccagcgtcgc acagcatgtc
    11161 tgcctgtcgc cgtcgcgtct gtcgcatttg ttccgccagc agttagggat tagcgtatta
    11221 agctggcgcg aggaccagcg catcagtcag gcgaagctgc ttttgagcac cacccggatg
    11281 cctatcgcca cagtcggtcg caatgtcggt tttgacgatc aactctattt ctcgcgagta
    11341 tttaaaaaat gcaccggggc cagcccgagc gagtttaggg ctggatgtga ataataagaa
    11401 agaaggggga gtct
//
LOCUS       DAOFSV010000139        11304 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.139, whole genome shotgun sequence.
ACCESSION   DAOFSV010000139 DAOFSV010000000
VERSION     DAOFSV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.139"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            276..842
                     /locus_tag="QBH27_003817"
     CDS             276..842
                     /locus_tag="QBH27_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7016078.1"
                     /translation="MNKKLLSLLVSGSLIAVVSTSVFATDTGVVNFNGKIVADTCEIN
                     VNESASTTGTVTFADTYPADYSGDGSIGTSKDFKIELIKCDPLVDKLNLTFSGTTTDA
                     GFLRLENSEAGAGSATNVGITVTNKNGGANDVKFDGSVPDASTDVDNAGSITPTIFNY
                     TANVIQVGNNAPTAGKYASSATFEVFYR"
     gene            888..1604
                     /locus_tag="QBH27_003818"
     CDS             888..1604
                     /locus_tag="QBH27_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ7016079.1"
                     /translation="MSVFSRVCLGFTFLLTALQVQASIVLGGTRIIYPSNQNEVQITL
                     KNKDAYARYLVQSWVSNIDGSKAPFLITPPVYKLEENRQTLLHIVFTGDKNKLPQDRE
                     SLFLANIKSVSAMPEELKDRNTLQFAMKARLKLFWRPASLDNSDALTAWEKLKFHKEA
                     GKLIVKNPTPFYISFSDLTVSGKNIVPTESKSEPGALLMMVGPFSEQRFSLPVGAGSI
                     VEWSVITDYGSSSGKRQQKL"
     gene            1671..4148
                     /locus_tag="QBH27_003819"
     CDS             1671..4148
                     /locus_tag="QBH27_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ7016080.1"
                     /translation="MLLPLHRTFVLTGITFALSAVYSLSYARDEFNLRILELDSPLEN
                     TQVLEDFVNNNNLTPGVYLTSVMWGQEYLDKRNITFILSSDKKRLIPRFTKADLREFG
                     LKVDDIPALQVMDDDTEFGDIAQIIDGARYDFQLDSQTLCLRIPQIYQNARAAGSISP
                     KYWSDGESAVWLSYYASGSRQNSDGDNLNSNWLNLNSGINLGVWRLRNNTVYSDSSWE
                     SISTSLQRDIKALRSQMEVGQTFTNGDLFDSVQMTGIKLETDTSMLPDSEQGFAPVVR
                     GIANSDAQVVIKQNGYVIYQTWVSAGPFEIKDLSQVTAGADLEVTIKETNGQEHSFIQ
                     ASSTVPILQREGALKYSLATGKYRDNDNHAETPVFGVATAIYGLPYGITIYGGILGAS
                     IYHSGVTGIGADLGRLGSVSVDITAAETKFDDGRDDATGLSWRAQYAKDFPDTDTTVT
                     LASYRYSTSQFYTFQEALDQRDTPDDKGIYSYRQTNNRRNRLQINLSQNIGRWGSVYL
                     NGYQQDYWGMHGAERSIGMGYSTTWSNINWSVNYTLTKTPGMAGEQQFSLTLNIPLSR
                     WLPDSWAMYNVNRSDKSNTSHQLGIGGTALQDNNLSYNLQQSYTDNNVGYDASMNGRY
                     RSSVGEFGLGYSYDKNSRQWNYSAQGAVVAHAHGVTLGQSVQDSFAIVHINEGANVKV
                     QNAQGVYTDYWGNAIVPNMTNYRHNAITVNTQGHDSLDISDATQDVIPSKGAVVGVDF
                     DARSGIRALLTLVHNKERVPFGALLTLGNSTAIVGEDGEVYITGVQESMTFTVQWGKE
                     INQQCTGVVTVPEKYTTGIYKATMDCR"
     gene            4170..5180
                     /locus_tag="QBH27_003820"
     CDS             4170..5180
                     /locus_tag="QBH27_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7016081.1"
                     /translation="MKPGFVSYIRTLLTIIFLSWGVIPEVFAGCNISGNGGNPATVQF
                     SVPALVVNSDAVPGTIIATVNANSPSISVSCTANGEIWQGYTVLTNGDMRTDNPLNNV
                     FQTNVPGIGFRAAWANNTSAQLTAGYLMTPWHKGSSTVIKGYGYPITIHAAIQFIVTG
                     PVGNGTIDTSKLVADWKYDNRVIGELRFSSVPVNIQPGTCDLIEKNITVPLNDIGTDD
                     FDVNNVSPVVSDDRFKIQIEKCSQDVKVDYRFTSSGSTGVTGNNILNIESGSGMAEGV
                     GLQILDSNNNVMVFDTDYTPVQKTTLNQNVTIPLKARYIKMGSVKSGRVNSVATFEVY
                     YR"
     gene            5400..7649
                     /gene="fhuA"
                     /locus_tag="QBH27_003821"
     CDS             5400..7649
                     /gene="fhuA"
                     /locus_tag="QBH27_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDQ7016082.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPNDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSERDD
                     KEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            7701..8498
                     /gene="fhuC"
                     /locus_tag="QBH27_003822"
     CDS             7701..8498
                     /gene="fhuC"
                     /locus_tag="QBH27_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDQ7016083.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILIDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGVADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            8498..9388
                     /gene="fhuD"
                     /locus_tag="QBH27_003823"
     CDS             8498..9388
                     /gene="fhuD"
                     /locus_tag="QBH27_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDQ7016084.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            9385..>11304
                     /gene="fhuB"
                     /locus_tag="QBH27_003824"
     CDS             9385..>11304
                     /gene="fhuB"
                     /locus_tag="QBH27_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDQ7016085.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGMVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPTGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAG"
BASE COUNT         2766 a         2492 c         2983 g         3063 t
ORIGIN      
        1 tttatttaat gaatatacga ccattaatat atattttggg tttatgttat attttattac
       61 cttaaactct taaaataaaa acaaaaacaa cactttaaaa ttcatgtggt tattgtttta
      121 ttactgcagt ttgtatagac aggctatgtg ttttttgttc taatctaaaa ataatattat
      181 ttggtggata tatttctaat atcaatattt atcaagccct gaccagtcgc tctggaatgg
      241 aatcatgtgt attacctcat aattaaggga tttttatgaa taaaaaattg ctttctctgt
      301 tagtgtcagg aagtctcatc gctgtagtaa gtaccagtgt atttgctaca gataccggag
      361 tggttaactt taacggtaaa attgttgccg atacttgcga aattaatgta aacgagtctg
      421 ccagtacaac agggacagtc acttttgcag atacttaccc agcggactat tctggagacg
      481 gtagtattgg aacgtcaaaa gattttaaaa ttgaattaat aaaatgcgat ccgttggtgg
      541 acaaactaaa cctgacattt tctggaacga caacggatgc aggtttcctg cgtctggaaa
      601 atagcgaagc tggggctggc agtgcaacaa atgtgggtat tacggtgaca aataaaaatg
      661 gcggtgctaa cgatgtgaaa tttgatggtt cagttcccga tgcatccact gatgttgata
      721 atgccgggag cattactcca acaatattca attacaccgc caatgttatt caggttggta
      781 ataacgcgcc gactgcgggt aagtatgcct catctgcgac gttcgaagta ttttatcgct
      841 aatattcata gtgtttgaaa ggactgtttg accacggagg gttatttatg tcagtttttt
      901 ctcgagtttg tttaggtttt acgtttcttc ttacggcatt gcaagttcag gcaagtattg
      961 ttcttggcgg tacacgtatt atctatcctt caaatcaaaa tgaagttcaa atcacattaa
     1021 aaaataaaga tgcatatgca cgctatttgg tgcaaagctg ggtgagtaat attgatggct
     1081 ctaaagcccc ctttctcatt acgcctccag tttataaact tgaagaaaat cggcaaacat
     1141 tactccatat tgtatttaca ggtgataaaa acaaacttcc tcaggatagg gaaagtttgt
     1201 ttctggcaaa cataaaatct gtttctgcga tgccagaaga actaaaagat agaaatacct
     1261 tgcaatttgc aatgaaagcc cggcttaaat tattttggcg gccagcttct ttggataata
     1321 gtgatgctct cactgcatgg gaaaaactta agttccataa agaagcgggg aagctcatag
     1381 taaaaaatcc aacgcctttt tatatttcat tcagtgatct gacagtatcg ggaaaaaata
     1441 ttgttcccac cgagagcaaa tctgaacctg gcgcattatt gatgatggta ggaccatttt
     1501 ctgagcagcg gtttagtctt cctgtcggag cgggaagtat tgttgaatgg tcggtgataa
     1561 ctgactacgg ttcaagttct gggaaacgac aacaaaagtt ataaatcacc gaagtgtatt
     1621 actataaata accgatctgt atttataata ttacgggcgc aagggaattt atgctgctac
     1681 cattgcatcg cacatttgtc ttaactggga taacatttgc attatcggca gtgtattcat
     1741 tgagctatgc acgagatgaa tttaaccttc gtatcctgga gctggatagc ccacttgaaa
     1801 atacacaagt gctggaggat tttgttaata ataataactt aactccggga gtatatctta
     1861 cctctgttat gtgggggcaa gagtatcttg ataagagaaa tattaccttc attctgtctt
     1921 cggataaaaa aaggctcatc cctcgtttta caaaggcgga tctccgtgag ttcggtctta
     1981 aagttgacga cattcccgcg ttacaagtaa tggatgatga cactgagttt ggtgatattg
     2041 ctcagattat tgatggcgca cgttatgatt tccagttaga cagccaaacg ctgtgtctgc
     2101 ggatcccgca aatctatcag aatgcgcggg ccgcagggag tatttcaccg aaatactgga
     2161 gtgatggtga gagtgcggtt tggttaagtt attacgccag tggttcccgg caaaacagtg
     2221 atggcgataa cctgaattca aattggttga atttaaattc agggattaat ttaggcgtat
     2281 ggcggctacg aaataatacc gtttacagtg actccagttg ggagtcgatc agcaccagtt
     2341 tgcaacgcga tatcaaagca ttaagaagcc agatggaagt cgggcagacg ttcacaaatg
     2401 gcgatctttt tgacagtgtg cagatgacag gtataaagct tgaaacggac accagtatgc
     2461 tgcctgacag cgaacaaggc tttgcgccag tggttcgtgg tattgccaac agcgatgcac
     2521 aagttgtgat taaacaaaac ggctatgtta tttaccaaac gtgggtatcg gcagggccat
     2581 ttgaaattaa agacttaagc caggtaacgg cgggggccga tctggaagtc accatcaaag
     2641 aaaccaacgg gcaagaacac agttttattc aggccagttc aacggttcca attctacagc
     2701 gagaaggcgc attaaaatat agtcttgcaa ctggaaaata ccgtgacaac gataatcatg
     2761 ctgagacacc tgtttttggt gttgcaaccg cgatatatgg cttgccctat ggaataacga
     2821 tttatggcgg catacttggt gcctcaattt atcattctgg tgtgacaggg attggcgctg
     2881 atttaggccg tctggggtcg gtatctgttg atatcaccgc agcagaaacg aagtttgatg
     2941 atgggcgtga tgatgctaca ggactgtcat ggcgtgcaca gtatgcgaaa gattttccag
     3001 acactgatac gacagtaact ctggcaagtt atcgttattc tacatcacaa ttttacactt
     3061 tccaggaagc acttgatcaa cgtgatacac ctgatgacaa gggtatttac agttatcgac
     3121 agacaaataa tcggcgtaat cgtttacaaa ttaatctttc acaaaatatt ggtcgttggg
     3181 gctcggtcta ccttaacggt tatcaacagg attattgggg aatgcatggg gccgaacgca
     3241 gtataggaat gggatacagt acgacctgga gtaacattaa ttggtctgtt aattacacac
     3301 taacgaaaac gccaggaatg gctggtgagc agcaattttc attaacactc aatattcctc
     3361 tctctcgctg gctgccagat tcatgggcga tgtataacgt aaaccgttcc gataaaagta
     3421 atacatcaca tcaattggga attggcggta ctgctctgca agataacaac ctgagttata
     3481 atttacagca aagttatact gataataatg tgggttatga tgccagcatg aatggacgtt
     3541 atcgtagctc agtgggggaa ttcggtctgg gatatagcta tgataaaaat agtcgtcagt
     3601 ggaattacag tgcgcaaggt gcggtggttg ctcatgctca tggagtaaca ttggggcaat
     3661 cagtgcagga ttcattcgcg atcgtgcata ttaatgaggg tgccaatgta aaagtacaaa
     3721 atgcgcaagg cgtttatact gattattggg gaaacgcgat tgttcccaat atgacgaatt
     3781 atcgacataa cgctattact gtgaatacac aggggcacga tagtcttgat atttctgatg
     3841 ccacacaaga tgttattcct tcgaaagggg ctgttgttgg tgtcgatttt gatgcacgta
     3901 gcggaatacg agccttgttg acattagtcc ataataaaga acgcgtaccc tttggggcat
     3961 tgctgacatt agggaatagt acagcgattg ttggtgaaga tggggaggtt tatattactg
     4021 gtgtgcaaga atcaatgaca tttacggtac agtggggaaa agagattaac cagcaatgta
     4081 caggagttgt cacagtgcca gaaaaataca ccacgggtat ctataaagcc acaatggatt
     4141 gccgataaca aataagacag gaaaaaataa tgaagccagg attcgtctct tatatacgaa
     4201 cattattgac cataatattt cttagctggg gcgtcattcc tgaggttttc gccggttgta
     4261 acatttctgg aaatggagga aatccggcaa cagtacaatt ctccgtgcct gcgttggttg
     4321 ttaatagcga tgccgttcct ggaacgatta ttgcgacagt aaatgcgaac agtccgtcaa
     4381 tttcagtttc ctgtacggca aatggtgaga tctggcaagg atataccgta ttgacaaacg
     4441 gtgatatgcg tactgataac ccacttaaca atgtgtttca gactaatgtg ccaggaatcg
     4501 gttttcgcgc ggcgtgggcg aataacacca gtgcgcaact caccgccgga tatttaatga
     4561 cgccctggca taaaggctcg tcaacagtga ttaaaggata tggttatccg attacaatac
     4621 atgcagctat tcagtttatt gtcacaggac ctgtcggtaa tgggaccatt gataccagca
     4681 aactggttgc tgactggaag tatgacaatc gtgttattgg agagcttcgt tttagttctg
     4741 ttccggtcaa tattcaaccg ggcacatgcg accttataga aaaaaatatt accgttccac
     4801 tcaatgatat tggcacagat gattttgatg ttaacaatgt gtcacctgta gtttccgatg
     4861 atcggtttaa aattcagatt gaaaaatgtt ctcaggatgt aaaagttgat taccgcttta
     4921 cttcatcagg ttctactgga gtaacgggta ataatattct taatattgag tctggttcag
     4981 gaatggcgga aggagttggt ctgcagatcc tcgatagtaa taacaatgtg atggtatttg
     5041 atacagacta tacacctgtt caaaaaacga cgttaaatca aaatgttact attccgttaa
     5101 aagcacgcta catcaaaatg ggtagcgtca aaagtggtcg ggttaattct gttgccacct
     5161 tcgaagtgta ttaccgataa gcttttccct ttgccataaa tgttaatttt tggtaatgga
     5221 atttttatgg tttacggtgc aaagattaac atttttgatt catctgattg attctcgccc
     5281 cttcatggac gcacagagcg tatatcactt gcgaacccgc cagcgtttcg aatattatcg
     5341 catctttata ataatcattc tcgtttacgt tatcattcac tttcatcaga gatataccaa
     5401 tggcgcgttc caaaactgct cagccaaaac actcactgcg taaaatcgca gttgtagtag
     5461 ccacagcggt tagcggcatg tctgtttatg cacaggcagc ggttgaaccg aaagaagaca
     5521 ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc atgggggcca gctgcaacta
     5581 ttgcggcgcg acagtccgct accggcacta aaactgatac gccgattcaa aaagtacctc
     5641 aatctatttc tgtcgtcacc gctgaagaga tggcactgca tcagcctaag tcggtgaaag
     5701 aagcgcttag ctatacccct ggcgttgctg tgggaacccg tggcgcatct aatacttatg
     5761 attacctgat catccgcgga tttgcggcag acggccaaag ccagaacaac tatctgaatg
     5821 gcctgaagat gcagggcaac ttctataacg atgcggttat tgacccatat atgctggagc
     5881 gcgctgaaat catgcgtggc ccggtttccg tgctgtatgg gaaaagcagc ccaggcggcc
     5941 tgttgaacat ggtcagcaag cgcccgacca ctgaaccgtt gaaagaagtt cagtttaagg
     6001 ccgggactga cagcctattc cagactggtt ttgacttcag cgatgcgctg gatgatgacg
     6061 gcgtttactc ttatcgcctg accggccttg cgcgttctgc gaatgcccag cagaaggggg
     6121 ctgaagagca gcgttatgct attgctccag cgtttacctg gcgtccgaat gataaaacca
     6181 atttcacttt cctttcttac ttccagaacg agccggaaac cggttattac ggctggttgc
     6241 cgaaagaggg gactgtcgaa ccgctgccaa acggtaagcg tctgccgacc gactttaacg
     6301 aaggcgcgaa gaacaacacc tattctcgta acgagaagat gattggttat agcttcgacc
     6361 acgaatttaa cgacaccttt actgtgcgtc agaacctgcg ctttgcacaa aataaagtct
     6421 cgcaaaagag cgtatatggc tacggcatgt gctcagaccc gctctattct tcaaacccct
     6481 cttcgagtcc atgtgctaac gtgccgcagt ctcaatgggg ccatacactg actcgtcagt
     6541 acgttatcga taatgagaag ttagaaaact tctctgttga tactcagttg caaagcaaat
     6601 tcgcaaccgg ttcagtggat cacactctgc tgactggcgt tgactttatg cgtatgcgta
     6661 atgacatcga ttcctggttt ggttatgccg gttcagttgc accatctgat atctataatt
     6721 tagaccgtag tgactttgat tttggcgctc acccgaatcc gtctggtcca taccgcgttc
     6781 tgctgaaaca gaaacaaacc ggcctgtatg ttcaggatca ggcgcagtgg gataaagtgc
     6841 tggttacgct tggcggtcgc tatgactggg ccgatcagtc atctttcaac cgtgattacg
     6901 gtaataaatc tgaacgtgac gacaaagagt tcacctggcg tggtggtgta aactacctgt
     6961 tcgacaacgg cgtaacgcct tacttcagct acagtgaatc atttgaaccc gcttcacaga
     7021 ctgatgcgaa cggcgatctg tttgcacctt cgaaaggcaa acagtatgaa gtcggcgtga
     7081 aatatgtgcc agaagatcgc ccaattgtgg tgactggcgc actgtatcag cttaccaaaa
     7141 ctaataacct gatggcggat ccgaacggtt cactgttctc ggttgaaggc ggtgagattc
     7201 gtgcgcgtgg cgtagagctg gaagcgaaag cggcactgtc ggcgagtgtt aacttggttg
     7261 gttcttacac ctatactgac gcggaataca ccactgacac caactacaaa ggaaatacgc
     7321 ctgcacaggt gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc
     7381 cgctttcagg tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc
     7441 cggctaactc ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc
     7501 tggcgcgagt cggcatggcg ggctccaacg tggcgctgca tgtcaacaac ctgttcgatc
     7561 gtgaatacgt cgccagctgc tttaacactt atggttgctt ctggggcgca gaacgtcagg
     7621 tcgttgcaac cgcaaccttc cgtttctaat ttctcttttt ggggcacgga tttccgtgcc
     7681 cgtttcacaa gttggctgtt atgcaggaat acacgaatca ttccgatacc acttttgcac
     7741 tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt gcatccgctg tcgttaacct
     7801 ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg ttctggtaaa tccactctgc
     7861 tcaaaatgct tggtcgtcac cagccaccgt cggaagggga gattcttatt gatgcccaac
     7921 cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt ggcttatttg ccgcagcagc
     7981 ttcctccggc agaagggatg accgtgcgtg aactggtggc gattggtcgt tacccgtggc
     8041 atggcgcgct ggggcgcttt ggtgttgcag atcgcgaaaa ggtcgaggaa gctatctcgc
     8101 tggttggctt aaaaccgctg gcgcatcggc tggtcgatag cctttctggc ggcgaacgtc
     8161 agcgggcgtg gatcgccatg ctggtggcgc aggatagccg ctgtctgttg ctcgacgaac
     8221 cgacttcggc gctggatatc gcccaccagg ttgatgtact ggcgctggtg catcgtttaa
     8281 gtcaggagcg tggcctgacg gtcattgccg tgctgcacga tatcaatatg gcggcgcgct
     8341 actgtgatta tctggtcgcc ctacgcggcg gtgaaatgat tgctcaggga acgcctgcgg
     8401 aaattatgcg cggcgaaacc ctcgaaatga tttatggcat cccgatgggt attctgccgc
     8461 atccggcggg tgctgcacct gtgagttttg tttattgatg agcggcttac ctcttatttc
     8521 gcgccgccga ctgttaacgg cgatggcgct ttctccgttg ttatggcaga tgaataccgc
     8581 ccacgcggcg gttattgatc ccaatcgtat tgtggcactg gagtggttgc cggtggagtt
     8641 actgctggcg ctcggcatcg tgccttacgg cgtggcggac accatcaact atcgcctgtg
     8701 ggtcagcgaa ccgccattgc cggactcagt gattgacgtt ggtttgcgta cagaacccaa
     8761 ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc tggtctgcag gatatggccc
     8821 ttcaccagaa atgctggcac gtattgcgcc ggggcgcgga tttaacttca gcgacggcaa
     8881 acagccactg gcgatggcgc gtaaatcact gacggaaatg gcagatttac ttaacctgca
     8941 aagcgcagcg gaaacgcatt tagcgcacta tgaagacttt atccgcagca tgaaaccccg
     9001 ctttgtgaag cgtggtgcac gcccgttatt gctgacgacg cttatcgatc cgcgccatat
     9061 gttggtcttc ggtccaaaca gcttgttcca ggaaattctc gatgagtacg gcatcccaaa
     9121 tgcctggcaa ggggaaacca acttctgggg cagtaccgcc gtcagtatcg atcgtctggc
     9181 agcgtataaa gacgttgatg tgctctgttt tgatcacgac aacagcaaag acatggatgc
     9241 gctaatggca accccgctgt ggcaggccat gccgtttgtc cgcgccggac gctttcagcg
     9301 cgtacctgca gtttggttct atggtgcgac gctttctgca atgcactttg tgcgtattct
     9361 ggataacgcc atcggaggta aagcgtgagt aaacgaattg cgctttttcc ggtgttattg
     9421 ctggcgctgc tggtggttgc tgctgcggcg ttgacctgga tgaacttctc gcaggcgctg
     9481 ccgcgcagcc agtgggcgca ggccgcctgg tcgccggata ttgacgtcat cgagcagatg
     9541 atttttcact acagcttgtt gccgcgtctg gcgatttcgc tgctggtggg cgcgggcctg
     9601 gggatggtgg gcgtgctgtt tcagcaagtg ctgcgtaacc cgctggcgga accgacgacg
     9661 ttgggtgttg caacaggcgc gcaactgggg attaccgtca ccacgctctg ggcgatcccc
     9721 ggcgcgatgg cgagccagtt tgctgcgctg gcaggggctt gtgttgttgg cttaatcgtc
     9781 tttggcgtcg cgtgggggaa acggctttcg ccggtaacgc tgatcctcgc ggggctggta
     9841 gtgagccttt attgcggcgc aatcaatcag ttactggtta tcttccatca tgaccaactg
     9901 caaagcatgt tcctgtggag caccggaacg ctgacgcaaa ccgactgggg cggcgttgag
     9961 cgtttatggc cgcagctgct gggcggcgtg atgctgacgt tattgctact tcgcccgtta
    10021 actctgatgg ggcttgatga tggcgtggcg cgcaatctcg ggctggcctt gtcgctcgct
    10081 cgtctggcgg cgttgtcgct ggcgattgtc atcagtgcgc tgctggtgaa cgcggtgggg
    10141 attatcggct ttatcggttt gttcgcgcca ctgctggcga aaatgctggg ggcgcggcgt
    10201 ttgttgccac ggttgatgct ggcatcgctg attggtgcgt taattctgtg gctttctgat
    10261 caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt ctaccggttc ggtcactgcg
    10321 ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt tacgcagcat tagcgcgccg
    10381 gatatgaagg tcaacgatcg tgtcgcgact gaacgccaac acgtgctggc gtttgctctc
    10441 gcgggcggcg tgctgctgtt gatggctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg
    10501 cacggctgga cgtgggcgag cggggcgttg ctcgaggatt tgatgccctg gcgctggccg
    10561 cgaattatgg cggcgctgtt tgcgggtgtc atgctggcgg tggcgggctg tattattcag
    10621 cggctgaccg gaaacccgat ggcaagcccg gaagtgctgg ggattagctc cggcgcggcg
    10681 tttggcgtgg tgttaatgct gtttctggtg ccgggtaatg cctttggctg gctgttaccc
    10741 acaggcagtc tcggggcggc ggtgacgctg ttgatcatta tgatcgccgc cggacgcggt
    10801 ggattttccc cacaccgcat gttactggcg gggatggcgt taagtaccgc gttcaccatg
    10861 cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg cgcaagttct gacctggatt
    10921 tccggttcga cctacaacgc gaccgatgcg caggtctggc gtactggaat tgtgatggtg
    10981 attttgctgg cgattacccc gctgtgccgc cgctggctga ccattttacc gctgggtggt
    11041 gataccgcac gagccgtggg gatggcgctg acgccgacgc gaattgcgct gctgctgtta
    11101 gcggcttgcc tgacggcgac cgcgacaatg accattggtc cgttgagttt tgttggttta
    11161 atggcaccgc atattgcgcg gatgatgggc tttcgacgga cgatgccaca catcgtaatt
    11221 tcggcgctgg tgggaggatt actgctggtg ttcgctgact ggtgtgggcg gatggtattg
    11281 tttccattcc agatcccagc gggg
//
LOCUS       DAOFSV010000140        11293 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.140, whole genome shotgun sequence.
ACCESSION   DAOFSV010000140 DAOFSV010000000
VERSION     DAOFSV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.140"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(1..1048)
                     /locus_tag="QBH27_003825"
                     /pseudo
     CDS             complement(1..1048)
                     /locus_tag="QBH27_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1173..3848)
                     /gene="adhE"
                     /locus_tag="QBH27_003826"
                     /gene_synonym="adhC"
     CDS             complement(1173..3848)
                     /gene="adhE"
                     /locus_tag="QBH27_003826"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDQ7016086.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4325..4972
                     /gene="ychE"
                     /locus_tag="QBH27_003827"
     CDS             4325..4972
                     /gene="ychE"
                     /locus_tag="QBH27_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDQ7016087.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            5710..7341
                     /gene="oppA"
                     /locus_tag="QBH27_003828"
     CDS             5710..7341
                     /gene="oppA"
                     /locus_tag="QBH27_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDQ7016088.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRAALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            7427..8347
                     /gene="oppB"
                     /locus_tag="QBH27_003829"
     CDS             7427..8347
                     /gene="oppB"
                     /locus_tag="QBH27_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDQ7016089.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            8362..9270
                     /gene="oppC"
                     /locus_tag="QBH27_003830"
     CDS             8362..9270
                     /gene="oppC"
                     /locus_tag="QBH27_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDQ7016090.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            9282..10295
                     /gene="oppD"
                     /locus_tag="QBH27_003831"
     CDS             9282..10295
                     /gene="oppD"
                     /locus_tag="QBH27_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDQ7016091.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            10292..>11293
                     /gene="oppF"
                     /locus_tag="QBH27_003832"
     CDS             10292..>11293
                     /gene="oppF"
                     /locus_tag="QBH27_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDQ7016092.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
BASE COUNT         2746 a         2640 c         2904 g         3003 t
ORIGIN      
        1 ttaagcaact gactggctct ttttcggggc tgtggagtat ttccagggcc tctcctttat
       61 cactctcggg aaggcccttt ctcttcttgt cggtaatttc actagttgtc ccatacttgc
      121 aagatcgcgc atcagctccg gtatacgtcc cggggaagcg catggcgtcc gtcatcgacg
      181 tcagcagatg gcagactttt cctttgcgcg tcacggtcag caggcggact gtcacctcat
      241 ttcccagtcc cggccacttt tttcgtgcct gcgggctggt gttcagcttc accagatgat
      301 cgcctttacc cagttttctg atctcttcat attgcgctcc ctttctgaga gggatcatcc
      361 agtggcggtg ttctcccgcc aggctccagg tatttaacag tcccagtgag taataacctt
      421 tatccattaa cgtcagggtg ttatcgccgg tttgttctat aagttgctca gcaagctcat
      481 tttcgctgtt cttcatcgtg ccgaaggctg cagccgtcag cagatggctg gtcagttcca
      541 tctggcagac cattttgacc tgcaggtaga gcgccgggtt cccggcatgt gtctggcggg
      601 ggaaggctgc atcgttctct ggtgtatccg gtgtgcgcca gaacacacca tcgatggcca
      661 gcagggtcag gccgcaccag tgcggatgcg gcgtggtgtt atgccagagc tgcgctgttt
      721 tcgtgaacac gcggcggaca gcctcaattc ccagacgctg gcgggtctga ataacggcac
      781 tgggggcaac gaaggggcga ttgcccggca gcatgatatc caggcgattc acaatctggt
      841 gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc atttcgaggg
      901 gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga gagatgagtt
      961 cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg taacgggata
     1021 ccaaatcaag ggcctgtcca atgagcataa aaaaatccgg aaacgagtga gcgtttccgg
     1081 attcttacac agccactgga tcggtcaact gatccttaac tgactggcat taccccagaa
     1141 ggggccgtta tgttgccaga cagcgctact gattaagcgg attttttcgc ttttttctca
     1201 gctttagccg gagcggcttc tttcttcgct gcagtttcgc cttctacata atcacgaccg
     1261 tagtaagtat ccagcagaat ctgtttcagc tcggagatca gcgggtaacg cgggttagcg
     1321 ccggtgcact ggtcatcgaa tgcatcttca gacagtttat ccacgttcgc caggaagtct
     1381 gcttcctgaa cgccagcttc acggatagat ttcggaatac ccagttcagc tttcagtgtt
     1441 tccagccatg ccagcagttt ctcgatctta gcagcagtac ggtcgcccgg tgcgctcaga
     1501 cccaggtggt cagcaatttc agcataacga cggcgagcct gcggacggtc atactggctg
     1561 aatgcagtct gcttggtcgg gttgtcattc gcgttgtagc gaataacgtt acaaatcagc
     1621 agggcgtttg ccagaccgtg cggaatatgg aactgggaac ccagtttgtg cgccattgag
     1681 tgacatacac ccaggaaggc gttcgcaaac gcgatacccg cgatagtcgc tgcactgtga
     1741 acacgttcac gcgctaccgg atttttagac ccttcgtggt aggacgctgg cagatattct
     1801 ttcagtagtt tcagtgcctg cagagcctga ccatcagaga actcagatgc cagtacagaa
     1861 acataagctt ccatggcgtg agttactgcg tccagaccac cgaaagcaca cagggacttc
     1921 ggcatgtcca taacaaggtt ggcgtcgaca atcgccatat ccggggtcag tgcatagtct
     1981 gccagcggat atttctgacc agtagtgtcg tcagttacaa ccgcaaacgg agtgacttca
     2041 gaacctgtac cagaagtggt ggtgacagcg atcattttcg ctttcacacc cattttcggg
     2101 aacttgtaga tacgtttacg gatatccata aagcgcagcg ccagttcttc gaagtgagtt
     2161 tccggatgtt cgtacataac ccacatgatc ttcgcagcgt ccatcgggga accaccaccc
     2221 agcgcgataa tcacgtctgg tttgaaggag tttgccagtt ctgcaccttt acgaacgatg
     2281 ctcagggtcg ggtcagcttc tacttcgaag aagacttcag tttcaacgcc tgctgctttc
     2341 aatacggaag tgatctgatc agcatagcca ttgttgaaca ggaagcggtc agtcacgatg
     2401 agcgcacgtt tgtggccatc agtaatcact tcatccagcg cgattggcag ggagccacgg
     2461 cggaagtaga tagatttcgg aagtttgtgc cacaacatgt tttcagctcg cttagcaacg
     2521 gttttcttgt tgatcaggtg tttcggacca acgttttcag agatggagtt accaccccag
     2581 gaaccacaac ccagagtcag ggaaggtgcg agtttgaagt tatacaggtc accgatacca
     2641 ccctgagaag ccggggtgtt aatcaggata cgagccgttt tcattttctg accgaagtaa
     2701 gaaacgcgag ccggttggtt atcctggtca gtgtacaggc aagaggtatg accgataccg
     2761 cccatagcaa ccagtttttc tgctttttct accgcgtctt cgaaatcctt agcacggtac
     2821 attgccaaag tcggggacag tttttcatgt gcgaacggtt cgctttcatc aacaacggtt
     2881 acttcaccga tcagaatctt ggtgttttct ggtacagaga agcctgccag ttcagcaatt
     2941 ttataggctg gctgaccaac gatagccgcg ttcagcgcac cgtttttcag gataacgtcc
     3001 tgaacagctt tcagctcttt accctgcaac agatagccac cgtgggttgc gaaacgttca
     3061 cgtactgcgt cataaacaga gtcaacaaca acaacagact gttcagaagc acagattacg
     3121 ccgttgtcga aggttttgga catcagtaca gatgcaactg cacgtttgat atcagcagtt
     3181 tcatcgataa caactggagt gttgcccgcg cctacgccga tagctggttt accggagctg
     3241 tatgcggctt taaccatgcc cggaccacca gtcgcgagga tcaggttgat gtctgggtgg
     3301 tgcatcagtg cgttagacag ctcaacagaa ggttgatcga tccagccgat cagatctttc
     3361 ggagcaccgg cagcgatagc agcctgcaga acgatatcag ccgctttgtt ggttgcatct
     3421 tttgcacgcg ggtgcgggga gaagataatg gcgttacggg tcttcaggct gatcagtgat
     3481 ttgaagatag cagttgaagt cgggttagtg gtcggaacga tgccgcaaat aataccgatg
     3541 ggttcagcga tagtaatggt accaaaagtg tcgtcttcag acagaacacc acaggttttt
     3601 tcatctttat aggcgttgta gatatattca gaagcaaagt ggtttttgat cactttatct
     3661 tcgacgatac ccatgccgga ttcggcaacg gccattttcg cgagtgggat tcgagcatct
     3721 gcagcagcca gagcggcggc gcggaagatt ttgtctactt gctcttgagt gaaactggca
     3781 tattcacgct gggctttttt tacacgctct acgagtgcgt taagttcagc gacattagta
     3841 acagccataa tgctctcctg ataatgttaa acttttttag taaatcatct gctcgaatac
     3901 gagagtatag tcagtgcggt gatgatttgc ttaaccgatg gaaatcaaaa gcttactcgc
     3961 gctcacactc actgtgattt actaaaagag tttaaacatt agagttatta tctctaatgc
     4021 gtcacttcca ggtggcgtaa gcaagattac tcacttctgg gtactgatta cgtgatcaag
     4081 atcaaatttt tgcaaagctg acacctttca gcatggcttt tcgccattat agctaacagt
     4141 taacaaattg tagtatgatt tgttggctac attagcatgt tttgcacaac tagataacaa
     4201 taacgaatga tagcaatttt aagtagttag gaggtgaaaa atgctgtcaa aaggcgtatt
     4261 gtcagcgcgt cttttcaacc ttatttatgg ctaacattat ccggcttttg cttcggagct
     4321 aaccgtgatt cagacctttt ttgattttcc cgtttacttc aaatttttca tcgggttatt
     4381 tgcgctggtc aacccggtag ggattattcc cgtctttatc agcatgacca gttatcagac
     4441 agcggcagcg cgaaacaaaa ctaaccttac agccaacctg tctgtggcca ttatcttgtg
     4501 gatctcgctt tttctcggcg acacgattct acaacttttt ggtatatcaa ttgattcatt
     4561 ccgtatcgcc ggtggcatcc tggtggtgac aatagcgatg tcgatgatca gcggcaagct
     4621 tggtgaggat aaacagaaca agcaagaaaa atcagaaacc gcggtgcgtg aaagcattgg
     4681 tgtggtgcca ttggcgttgc cgttgatggc ggggccaggg gcgatcagtt ctaccatcgt
     4741 ctggggtacg cgttatcaca gcattagcta tctgtttggt ttctttgtgg ctattgcatt
     4801 gttcgcttta tgttgctggg gattgtttcg catggcaccg tggctggtac gagttttacg
     4861 ccagaccggc atcaacgtga ttacgcgtat tatggggcta ttgctgatgg cgttggggat
     4921 tgaatttatc gttactggta ttaaggggat tttccccggc ctgcttaatt aattcctttc
     4981 aaatgaaacg gagctgccat gctccgttta cttcgtcatt atttttactt tgttcctgcg
     5041 cagttatcaa aagcaaaagg aataggtaaa aatattcttc tcaaattaca gttagttata
     5101 aggatttcct taactgcttc tcctcaccat catgttattt tcgccacatc ataatcctgg
     5161 gcttgctgaa gaataattga aatgatatta gtaattccac tgcctttggt agaggaaagt
     5221 gctaaataat aatcaattgt taaattattg tgcatttcac tactggaact gtaatcagaa
     5281 aagagagaca tgcttaacta acctctattt gattgaattg aaagatgttt gttaaggcat
     5341 ggatgcaagc tatagattct ggtacggtca ataaaagaga attgcttaac aattttgcaa
     5401 aatgtattgg cgagtaagaa ccgcatttgg tactttccgg gcaaccgcca ggcgattctt
     5461 tattggtaat gagaataatt aacaattaaa gaccgtcgcg aaagaataat gtgtctcgac
     5521 aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga ataaataaag
     5581 tcggtgatag taatacgtaa cgataaagta acctgacagc agaaagtctc cgagcctgtg
     5641 cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa tgagggagtc
     5701 caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt tttggctgcg
     5761 cttatggcag ggaatgtcgc gctggcagct gatgtacccg caggtgtcac actggcggaa
     5821 aaacaaacgc tggtacgtaa caatggttca gaagttcagt cattagatcc gcacaaaatt
     5881 gaaggtgttc cggagtctaa tatcagccga gacctgtttg aaggcttact ggttagcgat
     5941 cttgacggcc atccggcacc tggcgtcgct gaatcctggg ataataaaga cgcgaaagtc
     6001 tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg gcacgccagt cacagcacaa
     6061 gactttgtgt atagctggca acgttctgtt gatccaaaca ctgcttctcc gtatgccagt
     6121 tatctgcaat atgggcatat cgccggtatt gatgaaattc ttgaagggaa aaaaccgatt
     6181 accgatctcg gcgtgaaagc tattgatgac cacacattag aagtcacctt aagtgaacct
     6241 gttccgtact tctataaatt acttgttcat ccatcaactt caccggtgcc aaaagccgct
     6301 atcgagaaat tcggcgaaaa atggacccag cctggtaata tcgtcaccaa cggtgcctat
     6361 accttaaaag attgggtcgt aaacgaacga atcgttcttg aacgcagccc gacctactgg
     6421 aacaacgcga aaaccgttat taaccaggta acctatttgc ctattgcttc tgaagttacc
     6481 gatgtcaacc gctaccgtag tggtgaaatc gacatgactt ataacaacat gccgatcgaa
     6541 ttgttccaga agctgaaaaa agagatcccg gacgaagttc acgttgatcc atacctgtgc
     6601 acttactatt acgaaattaa caaccagaaa ccgccattta atgacgtgcg tgtgcgtacc
     6661 gcactgaaat tgggtatgga ccgcgatatc attgttaata aagtgaaagc gcagggcgac
     6721 atgcccgcct atggttacac tccaccgtat actgatggcg caaaattgac tcagccggaa
     6781 tggtttggct ggagccagga aaaacgtaac gaagaagcga aaaaactgct ggctgaagcg
     6841 ggttataccg cagacaaacc gttgaccatc aacctgttgt ataacacctc cgatctgcat
     6901 aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa acattggtgt aaacgtcaaa
     6961 ctggttaacc aggagtggaa aacgttcctc gacacccgtc accagggcac ttttgatgtg
     7021 gcccgtgcag gctggtgtgc agactacaac gaaccaactt ccttcctgaa caccatgctg
     7081 tcgaacagct caatgaatac cgcgcattat aagagcccgg cctttgacag cattatggcg
     7141 gaaacgctga aagtgactga cgaggcgcag cgtgcagctc tgtacactaa agcagaacaa
     7201 cagctggata aggattcggc cattgttcct gtttattact acgtgaatgc gcgtttggtg
     7261 aaaccgtggg ttggtggcta taccggcaaa gatccgctgg ataataccta cacccggaat
     7321 atgtacattg tgaagcacta atggcaatac gtggggcagg agtgtcctgc tccacggtgt
     7381 ctgattttta tcgcattaca gaaggcacag gccagaaggt agggcaatgt taaaatttat
     7441 tctacgtcgc tgtctggaag cgattccgac gctatttatt cttattacta tttcgttctt
     7501 tatgatgcgc ctcgcgccgg gcagtccttt taccggcgaa cgtactttac caccggaagt
     7561 gatggccaat atcgaagcga aatatcatct taatgatccg atcatgacac agtatttcag
     7621 ctacctgaaa caactggcgc atggcgattt cggtccatcg tttaaatata aagattattc
     7681 ggtcaatgac ctggttgcat ccagttttcc cgtttctgcc aaactgggag ctgcagcatt
     7741 ttttcttgcg gtaatactgg gtgttagtgc tggcgttatt gccgcattaa agcaaaacac
     7801 caaatgggac tataccgtga tggggctggc aatgaccggg gttgttatcc ccagttttgt
     7861 ggttgcgcca ttattagtca tgatatttgc gatcattttg cattggctgc cgggcggtgg
     7921 ctggaatggt ggagcgctta aattcatgat attgccaatg gtggcgttat cactcgccta
     7981 tatcgccagt attgcgcgta ttacccgtgg ctctatgatt gaggttttac actccaactt
     8041 tattcgtact gcccgggcga aagggttacc tatgcggcgg atcattttac gccacgcatt
     8101 aaaacctgct ctgttacccg tgctctccta tatggggccc gcatttgtcg gcattattac
     8161 cggttcgatg gtcatcgaaa ccatttatgg tttaccgggg attgggcaat tgttcgttaa
     8221 tggcgcattg aaccgtgact attccttagt gttaagtctg accattctgg ttggcgcgtt
     8281 aaccattttg tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc cgaaaatccg
     8341 ttactgatac tggagctcgc gatgatgtta agtaagaaaa acagcgagac gctggaaaat
     8401 ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc aggacgcacg tcgacgtttt
     8461 atgcataacc gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc gttatttgta
     8521 atcctggcac cgatgctttc gcagtttgcc tatgacgata ctgactgggc gatgatgtcc
     8581 agcgccccgg atatggagtc cggtcactac tttggtactg actcatccgg tcgcgacctg
     8641 cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc tgcggcactg
     8701 gtggcagtgg tcgtggggac actttatggt tcgctttccg gttatctggg cggtaaagtg
     8761 gattcggtaa tgatgcgtct gctggaaatc ctcaactcct tcccattcat gttcttcgtc
     8821 attttgctgg tgaccttttt cggtcaaaac atcctgctga ttttcgtggc gattggcatg
     8881 gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa ccctgagtct gaagcgcaaa
     8941 gagtttattg aggcggcaca agttggcggt gtatcgacgc cgggcattgt tattcgccac
     9001 attgtgccga acgtactcgg tgtggtggtg gtctatgcgt cgcttctggt gcccagcatg
     9061 atcctctttg aatctttcct tagcttcctg ggattaggta cacaagagcc gttaagcagc
     9121 tggggtgcat tgctgagtga tggcgcgaac tcgatggaag tctctccatg gttactgttg
     9181 ttcccagcgg gattcctcgt ggtaacgctg ttttgtttca actttatcgg cgatggcttg
     9241 cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc catgagcgta attgaaactg
     9301 caactgtgcc gctcgcacaa caacaggctg acgcactgct gaacgtgaaa gatttgcgtg
     9361 tcacctttag taccccggat ggcgacgtca cggcggtcaa tgatttgaat ttttcgctac
     9421 gtgccggaga aacgctgggc atcgtaggtg agtctggttc gggcaaatcg caaaccgcat
     9481 tcgcgttgat gggcctgtta gctgccaacg gacgtattgg cggatcggca accttcaatg
     9541 ggcgtgaaat cctcaatttg ccagagcatg aactcaataa actgcgagct gaacaaatct
     9601 caatgatttt tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc ggtgagcagt
     9661 tgatggaagt gctgatgctg cataagaaca tgagcaaagc tgaggcgttt gaagaatcgg
     9721 tgcggatgct cgatgcggta aaaatgccgg aagcgcgtaa acgcatgaaa atgtacccgc
     9781 acgaattttc tggcggcatg cgtcagcgag tcatgattgc gatggcattg ctatgtcgac
     9841 ctaagctgct gattgcggat gaacccacta ctgcgctgga cgtcaccgta caggcgcaga
     9901 tcatgacgct attgaatgaa ctgaagcggg aatttaatac cgccatcatt atgattaccc
     9961 acgatcttgg cgtggtggcg gggatctgcg acaaagtgct ggtaatgtac gccgggcgca
    10021 cgatggaata tggcaacgcg cgcgatgtct tttatcaacc cgtacatcct tattctatcg
    10081 gtttgctcaa cgcggtgccg cgtctcgatg cggaaggtga aacaatgttg actattccgg
    10141 gtaatccgcc aaacctgctg cgattaccga aaggttgccc gttccagcca cgttgtccgc
    10201 atgcgatgga tatttgtagt agcgcaccgc cgctggaaga gtttacgcct ggccgtctgc
    10261 gtgcttgctt taaaccggtg gaggaactgt tatgaatgct gtaaccgaag gaagaaaagt
    10321 cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc aaagatggca aacagtggtt
    10381 ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgta actcttcgcc tgtatgaagg
    10441 ggaaacatta ggtgtggtag gggaatcggg atgcggtaag tccacctttg ctcgcgccat
    10501 cattggcctg gttaaagcga ccgacggtca tgttgcctgg ttaggtaaag agttgctggg
    10561 catgaagccc gatgaatggc gtgccgtacg cagtgatatt cagatgattt tccaggatcc
    10621 gttggcatcg ctgaacccgc gtatgaccat cggcgagatc atcgctgaac cactgcgtac
    10681 ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga tgatgctgaa
    10741 agtcggtttg ttgcctaacc tgattaaccg ctatccgcat gagttctccg gtgggcagtg
    10801 ccagcgtatc gggattgctc gcgctcttat tcttgaaccg aagctgatta tctgtgatga
    10861 gccggtgtcg gcgctggacg tgtcaattca ggcgcaggtg gtcaacctgc tccagcagtt
    10921 gcaacgtgag atgggattgt cattaatttt tatcgctcac gacctggccg tggtaaaaca
    10981 catttccgat cgtgtgctgg tgatgtatct cggccatgcg gtagaactgg ggacctatga
    11041 tgaggtctac cacaatccgc tacatcctta caccagggca ttgatgtcgg cagtccccat
    11101 acctgatccg gatctggaga agaacaaaac catccagtta ctggaagggg aattaccgtc
    11161 gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt tgtccgattg caggtccgga
    11221 gtgcgccaaa acacgtcctg tgctggaagg gagtttcaga cacgccgttt cttgcctgaa
    11281 agtcgatccg ctt
//
LOCUS       DAOFSV010000141        11265 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.141, whole genome shotgun sequence.
ACCESSION   DAOFSV010000141 DAOFSV010000000
VERSION     DAOFSV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.141"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(98..889)
                     /locus_tag="QBH27_003833"
     CDS             complement(98..889)
                     /locus_tag="QBH27_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDQ7016093.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            complement(896..2866)
                     /locus_tag="QBH27_003834"
     CDS             complement(896..2866)
                     /locus_tag="QBH27_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDQ7016094.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            3148..3584
                     /locus_tag="QBH27_003835"
                     /pseudo
     CDS             3148..3584
                     /locus_tag="QBH27_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4109..4381
                     /locus_tag="QBH27_003836"
     CDS             4109..4381
                     /locus_tag="QBH27_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HDQ7016095.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
     gene            4827..5002
                     /locus_tag="QBH27_003837"
                     /pseudo
     CDS             4827..5002
                     /locus_tag="QBH27_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5228..6397)
                     /locus_tag="QBH27_003838"
     CDS             complement(5228..6397)
                     /locus_tag="QBH27_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016096.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVM
                     NIKSILVLVCCTTILSLIMIIFIFFITSFFIPIVIPYDIKNLPAYNKIQGNFLSVMML
                     SGLFPMAFIYVLYCAFDFDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKN
                     NRMKLLRRVQIYLIIPLSIALLVHLQVIPLEMVFRNIETSDKSINFKVIAELAFMSYF
                     IFILTILPGLIYLKMNPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKD
                     IIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
     gene            6764..6952
                     /locus_tag="QBH27_003839"
     CDS             6764..6952
                     /locus_tag="QBH27_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDQ7016097.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPRHDWL
                     NTGSLLQNIPLRIWFYPQ"
     gene            7073..7813
                     /locus_tag="QBH27_003840"
     CDS             7073..7813
                     /locus_tag="QBH27_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ7016098.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(8098..9075)
                     /locus_tag="QBH27_003841"
     CDS             complement(8098..9075)
                     /locus_tag="QBH27_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDQ7016099.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            9861..10592
                     /locus_tag="QBH27_003842"
     CDS             9861..10592
                     /locus_tag="QBH27_003842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016100.1"
                     /translation="MHRLDEVVDTLLVLQKKHRIRFDVWQVVKRDHAIISFFDQGMNP
                     AVPRVAYWTPFRYPLLLNLASLFDNELAEKAWCARLEAHDGRSSSLFSEVCSELLARV
                     HTLGDRRYIELITDALSWAMTHFDELGYNCKTGKQKLQIMPNMVGFQFVLRGICSRLG
                     APNRKADIVVDQQSQFNTTQRELREFYYQIREMPWVHGPGLPVMDVTNMPAERWFSSR
                     VQKVQGWSWLIYISGSSNALWKGRS"
     gene            complement(10961..>11265)
                     /locus_tag="QBH27_003843"
     CDS             complement(10961..>11265)
                     /locus_tag="QBH27_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDQ7016101.1"
                     /translation="STSKTPSYTKSVSWQHHRTNDNVPGLLSLITAHLKDLPDDGRNE
                     DVFKMLRSSAAILHGINNLRNNYSMAHPTETLLNEADARFAINLVRSIMTYVDELL"
BASE COUNT         3059 a         2543 c         2551 g         3112 t
ORIGIN      
        1 tgctcccctg gcgcgtagca ctgccgactg aataacacat ccccgtcaat acggttcttg
       61 ctgcacgctt acaatttcag ggcagggaat agtattgtta atttcgggag aaacttaaag
      121 ttgccacatc gacagtttca tcacacaggc ttttgtcctg aaaacttttc ttattttgga
      181 ctttcgccag ccactgtcca atatgaagag aaggtactgt gacgatacca tcctcattag
      241 ttacaaaggt gagttctgct ttatgaacag gatgtttgtg ctcaactgca tgagtatgac
      301 gggatgattt cagataactg accgagtcaa gctccatgat agctccggcg agcggggacg
      361 ttttatacag gactttcagc ttcacatctt cactgaaacg acttatatcg gataacggaa
      421 tgatttcaag ctcatgcccc gttggagact gatagagttg tgctggcatc tgctgttcac
      481 caggaataaa agatttcgca tatttagtga caaactcaca gtaagccaca tcatttctgc
      541 cctcccggct gaccggtttc catcctgaag aggttctcga ccagtatccc ggtttcatat
      601 atgttgacaa aacgtagcca ttatctttgc ttgcagactc agttttatag acataatccg
      661 tggacgctgg cttcagtgtt attgtctctt tcccgttata aatccgggga ttttcaaaga
      721 aattccttct gtcaggaatt gtgccctttg aggggaaatt atgaccataa ccaatctctg
      781 cggtaatgtt gttctctgcc tgtttacctg taatccagac atcgtgggcc aggacatgcc
      841 ccgatgctgt cagacaaaga gctaagatat aacctgtatt tttcatcatg atttgttacc
      901 actgataacg ggtataaaga ctgacagttc tgcctgaaag cggcactcct ttgccattca
      961 tggcaagctt gttgaaggca ttatccagag tcactgatgc ctcaaagcct ttaacactta
     1021 caggcttata agacagtgaa aaggaatgta cggcatagcc agcctgatgg ggatcgcctt
     1081 ttttcacatg cgtggctgct gccgaaagtt cagcacgcca tccggcagaa actcctccat
     1141 gaccaacagg tatacttatg tcactgatga gtgtgtcagg attgccggag cccagccatt
     1201 ccctgctgct ggcatccata ctttccgtgc ggttataaga aagcatccag tcaaaataat
     1261 cagactggta atttccctgc atatcccatc cccagatacg ggcccgggat acattataag
     1321 aataagaacg catccgtttc atatccacgc gcgtggcaat atagtttctg gcatccgttc
     1381 cgaaatatcc gcctttcaac tgcagccgat cattgcctgt aagcaggccg tccagttgaa
     1441 tacctgcgcc aatctcacgg gtaatgttat tttctggttt cagattaagg ttaggtaccc
     1501 agtaattggg tttacccttg cggtaaaaat gtacatcatc cctgtacatt tctgccattg
     1561 tgggggcacg gaatgcagag gatatggagc cgtacatcat cagccagtct gtcggtgaga
     1621 ctgaaatcgc cgcccttggg gacaggcgtt cggctttcag ttctccggca cggggattga
     1681 agctcttgta ccggtcatag cgggaaccga cgataatgtt aaccgggtag cttttcattg
     1741 tcatttcatc ctggaaatac aacgatgtaa agtcaatgtt tccttccgga tatagtgtgg
     1801 cagagccttc tggcttctgc tgctgacggt aatattcagc gccataactt aactgatggg
     1861 ctcccggaaa aatcagagta tgactcctgt tgacaacatt aacaccggag gttctgttgt
     1921 ttctccattc tgccgttttg ttgtgaccgt ttgtcttgat agtgatatca ctgagatata
     1981 aagttgactt tacattgatg aggctgttat ccacaggagc gtactggtac cagaactgta
     2041 cgtcattttg tactgttttt ctgtcacgca ggccgctgtc accatgtgtc tgagtgggat
     2101 tgcccggttc agtggtgtta ttccggtaga gacgcaagct ggcacctgct ttgttactgt
     2161 ctgttatacc gacagagcct ttcgcaaaca gggctgcagg tttttccttg ttaggtgcgc
     2221 gctcaccatc actttgatag atattacctc ttttacgcat aatgacagaa aggagcgcat
     2281 ctgtttttcc ggttttaccg aaccaggtga gccccgagcc tgtgctgtgg tcaccactgg
     2341 cgatatttcc ccacagactt aaaccgtttg tctctccggg gggaaggaaa tctgctgcat
     2401 cggcagttct gaagtccaca acacctccca gcccgccact gccgtacaga ctggagtttg
     2461 gtccgcggac aacttcgata cgtttgacga gtgccggatc cagataagtg ccactgcttt
     2521 ttgccatgtc actgagttga cggatgccgt caacaagaac aagtaccccg cttttgtcat
     2581 agccgcgtag attaaaggtc tgcccattgg tccgtcctgc accagtctgg ctgagaccgg
     2641 caacaccttt cagcatgccg gctgatgtga ccgcattctg actccatggc gtgtcattcg
     2701 tgacgacact gaccattgac ggcgtttcaa ataccgtgtt ctgatttcct gtcgcgacaa
     2761 cggtgattgt atctttagcc atagaagaat gactggtctc tgcctgtaac cctatcggga
     2821 gtatgattaa tgtacaaatg acgtttctga ttacgttcat atacatgcga aaatatatgt
     2881 taatattgat atgttataac atatcatttt attaatgcaa ccctgatcac gcagcgttca
     2941 gtgataacca gtgaatagcg actctaatac ccggtcgctc tgcaatgtat cagcgcctgc
     3001 gaccgatgct atggatattg atttttctac cgataaagaa ccagcaatac ggaatttaga
     3061 ctggccccct gaatctccag acaaccaata tcacttaaat aagtgatagt cttaatacta
     3121 gtttttagac tagtcattgg agaacagatg attgatgtct taggaccgga gaaacgcaga
     3181 cggcgtacta cacaggaaaa gatcgctatt gttcagcaga gctttgaacc gggaatgacg
     3241 gtctcccttg ttgcccggca acacggtgtg gcagccagcc agttatttct ctggcgcaag
     3301 caataccagg agggaagtct tactgctgtg gccgcaggag aacaggtcgt tcctgcctct
     3361 gaacttgctg ctgccatgaa gcagattaaa gaactccagc gcctgctcgg caaaaaaacg
     3421 atggaaaatg aactccttaa agaagccgtt gaatatgggc gtgcaaaaaa gtggatagcg
     3481 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
     3541 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggaaatat aggggcaaat
     3601 ccacatcctt acttttccgt aaggatttta gcgggtcaaa acagcgttcc atgccggact
     3661 gatgatctac atacttagag tcacatacca aagtaaaact atggatgtat gcaagcctgc
     3721 atcgtcagga ggttaaattt cagacctgcc agttaccgcg taaatggctt gatatatgac
     3781 taatttattt gcacaggatt tttacgacaa cgctctgaac cagcagttga cgggatatat
     3841 agttgagctc attacagata aagcttttct gtatctctgg cggtggtcat agaccagtaa
     3901 tcgttattgg ttgttctata ttatcccgta tgacggtaca acgtttttaa caactggcgc
     3961 tgtcacaaca taagccaccc caaaatatta tatgagtaac aatagtctat ataggagcgt
     4021 aaattaccta ctatcaaata cttactccaa tagcatttct gtctattcta tttcaattct
     4081 ttaatagaaa aataaggtta gataatatat gactccctcc ccctatccaa gaaataactt
     4141 taatacagag ctaattcaat catgtatgaa tggagaacac ttttcacttt taattgaaat
     4201 atcaccaatc agaagtaaaa aaaacatcat ggctcttaag gagtatttgg ttgatggtta
     4261 ttcaaagcaa gaaagttgtg aaaggaacaa tgtttccatt agttattttt gcctatgcct
     4321 aaaaaaacta aactatacac acagtattgt tagtgctctt aaatatttct accgtgagta
     4381 aactgtaact gaagtcttga ttcttacgct tagacactgc taagccgtta tggcatagct
     4441 gataatttat attgcactat gtcagctact gtaagataat ataagctatt ttaaagaaaa
     4501 ctctgtcatt catcattttg tttaatgata ggttttgtta ttaatataaa agaacatgct
     4561 cattcattaa aataaaaaca agatgttctt ttattatttt cattccctac ggggttagtc
     4621 cttgattcat tttcagtatc ttctaactca ttcatttgcc gattacttta attcacagtc
     4681 ttgtttttta tcctgcctat tggagataaa agtggtatcg atggatgccg agtgtgttgc
     4741 acatgcagta ttctcaggaa taagaactgt cacttccaaa acacgcgata tatgattgaa
     4801 tctctgtggg gggataaaaa cttgcctttt tccgcagtct gaaaacagga tgggggcgac
     4861 ggatggttac gcaggcaagg gcgaggttcg gaaataaatc agagattata ttctgaatta
     4921 ttacaatagc accagacctc accattacaa cggtagtttg acgctggagg gatcagaaaa
     4981 cagataccgt ttttactgta aacggtggtc atatatggac tctcctggtg atataagtac
     5041 tggttcgaaa cggtttacga ctatatatcc ggcgtcatca gtaggttcaa tgaaccgcgc
     5101 aataatgtta tccgagccct gtttcctaat cactacgccg gtatttgatt agccaagtca
     5161 agttcatctt gccatcacta tttttccata ccgcattact atctaataca gtcgcacgag
     5221 cggccttcta atttgagggt ttcgagccct gaagaggcat atcccatcgt tttacagata
     5281 ttgcagaaat tggtacgcaa tatgctgctt cttcctggag tttcttcctg gtatttgtat
     5341 cgaagtctac attaagcagg tcgaacttcc agctttcccg ataaaacctt ataatatcct
     5401 tgggacaaag gagaataaac tggtttgtcg taaacataga aacagcctgc acagagtaat
     5461 attcccccgg ttttatattt tttttacccc atactgcatt ggaaaaaaac tcctccggat
     5521 attcacttgt tttaataatg tagctatgta ttttgaagtc acttatacct gagagcttaa
     5581 taactgaatg ggtaaaaatt actggtaata cggttatttg agtcgatata actaataata
     5641 tcatcaagga tgcgacaaat gcagaactaa ttcttttaga aagtttatgc tgtggattca
     5701 tttttagata aatgaggccg ggaagtattg tcagaataaa aataaaatat gacatgaaag
     5761 ccagttcagc tatcactttg aaattaatac tcttatctga agtttcaata ttcctaaaaa
     5821 ccatctccag tggaattacc tgaagatgca ccagtaaagc aatagacaac ggaatgataa
     5881 gatagatctg aacacgtctc aacagtttca ttctattatt tttaaacgat aagtcatatt
     5941 caagatacct cgtgtttatt atggctgaaa taacaactat tagtactcct atacttaaaa
     6001 tagataacca gccagagtta tctttaacac tctggtcaaa atcaaatgca cagtataaca
     6061 cgtatataaa ggccatgggg aagaggccag ataacatcat tacagataaa aaattaccct
     6121 gaatcttatt gtaagcagga agatttttta tatcataagg aataactatt ggtataaaaa
     6181 aagatgttat aaaaaaaata aaaatgatca taatcaatga tagaatagtt gtacaacaaa
     6241 ccaataccaa gatgctttta atattcatca catcaaagaa aatatcaagt cgatccagac
     6301 gactgaaata tccccagaga actatcaacg caatccctga tataatactt gatggtgtta
     6361 atactgaaac tattagcgtc ttccacttct gattcatttt aacttcctgg ttttgctata
     6421 aagagagcat aaaaaagagt gaataagtta ttacacttca ttgtttgact cctcgcagta
     6481 acctttcagg aaggttgtcg gatatttttg ataaattatg tcataaacat attattgtaa
     6541 gtaatgattt cactaaattt atttgatcgt cgtcataata tttttaatct gaatatagaa
     6601 gatgattgtg tcatatgcag tatgatataa aacgttaaaa gtgactgaat agataacagt
     6661 tcatgcatga acaagaccat aacataacat aataaaatat gcttattatg cataacctta
     6721 tgttgtgact aagccgcctt aaaactgaga agtagctaag aatatggcgt cagacacctt
     6781 cagttgtcgt agaaggttat ctgcaggatt tttctccgta tctgcctgtt cctggcatta
     6841 ccccaattct cccaaaaacg gcaaaccagc cctcagcccg cgccacgact ggcttaatac
     6901 gggatccctg ctgcaaaaca tacctttacg tatttggttt tacccgcaat aatgcgtgat
     6961 aagcaaacaa aaggatccgc gcgctgcgat ttattgtcgt ggaaggatcc gggcgttcag
     7021 gtcagaatac ctgtatccat aaacagtgcg tgtgctacgt gaaaaataac tcatgaacaa
     7081 tgtcattccc ctgcagaatt caccagaacg cgtctccctg ttacccattg cgccgggggt
     7141 ggattttgca acagcgctct ccctgagaag aatggccact tccacggggg ccacaccggc
     7201 ctacctgctg gccccggaag tgagtgccct tctcttctat atgccggatc agcgtcacca
     7261 tatgctgttc gccaccctct ggaataccgg aatgcgtatt ggcgaagccc ggatgctgac
     7321 gccggaatca tttgacctgg atggagtaag accgtttgtg cggatcctgt ccgaaaaagt
     7381 gcgtgcgcga cgcggacgcc cgccaaaaga tgaagtgcgc ctggttccgc tgacagatat
     7441 aagctatgtc aggcagatgg aaagctggat gatcaccacc cggccccgtc gtcgtgaacc
     7501 attatgggcc gtgaccgacg aaaccatgcg caactggctg aagcaggctg tcagacgggc
     7561 cgaagctgac ggggtacact tttcgattcc ggtcacacca cacaccttcc ggcacagcta
     7621 tatcatgcac atgctctatc accgccagcc ccggaaagtc atccaggcac tggctggtca
     7681 cagggatcca cgttcgatgg aggtttatac cagagtgttt gcactggata tggctgccac
     7741 gctggcagtg cctttcacag gtgacggtcg ggatgctgcg gagatcctgc gtacactgcc
     7801 tcccctgaag tgacggcata cgcgcttcct tatataagct gtggtcagca gaacagacat
     7861 aacataagct ggagcaggta gataagctgt agtgagtaaa ccatgtttta tgaagggagc
     7921 aatgcctcag catcaggtta cggggtgact cacgtaaggg acaggcagat ggcagctcag
     7981 ccacaggcag cactgcagga aactgaatat aaacagcagt gagcagacca ctcactgcac
     8041 ctgataatat aagctgtagt cagtaaagga gcactcttca ctgactacag tttatgttca
     8101 gcgggatttg aagagttttt ccaggtcatc cagtgtgatg cccagttttt caagcagggc
     8161 aagtttctcc gccatctccg gactgacttt ttccgcaggt gaaggtggca acggattttc
     8221 cttactctca tcattcggcg cttttaaccg gggacgccgg tagtgaatac agaagaattt
     8281 tgtccgcccc cgctggatct ccgtgtaatc aagatatcca atctcgcgca actgctccat
     8341 tgcccgtctg accgtctggt tctgggaaaa tacaggagac ttcagattga ggcgtgcacg
     8401 cagccgcgcc agcgatatcg gtgccggatc ccggggcagg ctctctataa aggtgtagag
     8461 tgcctgggcg gactcccgtc gcttcagggc attaatcgcc ttaagctgga gaaggacttt
     8521 tctgtcaaac tggtacagtt caaacaggcg gggatcagcc tgtaactgaa caatatcccg
     8581 ttcagtatcg tagtaggctg actgtaccag atgggtgatg tattcccggg tgtgcttctc
     8641 atcggtacgg gaaaacgaga tcacggtacc ggcaatgcgc ttcagggaag ggctgatgcg
     8701 ctcacgcagc ctgcgggatg actggcttga aggtatacca cacagttttg caaactcaac
     8761 aaaaggcagt tcaactttgt caccaatcac gttatggcgg gcaaaggaat gaatgatccc
     8821 cacccaggtc ttgaaatcgt tatccatatc cagccggggg ccggtgatct caaccttatc
     8881 gaatccttct gcacgggcca gagaaagacg cgtcagttct tccgtggcat cggtgcgtga
     8941 cagtgtattt tttttactgt tcttcagtga cttaagggtc ggtacaaaga cgcccagacg
     9001 catcagcgcc acaggttgta cggtgttgtt attgtttggt gtcagtgtca ccagctcacc
     9061 ggacgactta tccacctcgc cgaacagttt tttgatgtct gaattttcgt tttccagaaa
     9121 gcctccagcc tgtggacagt aaaagaataa acagaaaatc gtgtgtcaga actccgctaa
     9181 ctgactgaag aaaaacctgc agcctgtatt taacaactga gtctggcata ctgaccatag
     9241 tttatgtcat taacttactt cagtatatgg attactgaat acagcttatc attagcatgt
     9301 tacagtttat cagtaacatg ccacagctta tgtgatcgta gtcccggccc atgagtgaca
     9361 aggtttgcag agatatgggg atctttaatg gatcctttgt tgatcattca tggatctgtt
     9421 tactggatcg ggcctccgga tatgtggata aatcattcca gaaaaacagc atacactacc
     9481 gattatgaag gcttcagggc gtacctgctc actgtaacta cagatccatt gcgcttcctc
     9541 gccggttcgc gggcttcgcc cggtcaccgg tctgcggcga gcactgtttt tctgatgtct
     9601 gtctttacag gtaatttttg acggaatctg ccgtacttcc tgagcgtacg gtcatatccg
     9661 aagtacactt catatgtgtg tttcaatgcc agggattgtg tatacaaact acaggcaaag
     9721 tctggttatc accggtggta ggctgctccg atggaaaaat tgtgaacgga ttgacaccat
     9781 gccggataac tctgtcgata taagacggtc aaagatcgtg gcgttgacaa gtacagcgtc
     9841 tgcacgccgc agagcttggc atgcaccgcc ttgatgaggt ggttgatacg ctgcttgtcc
     9901 tgcagaagaa gcaccggata cggtttgatg tctggcaggt cgttaaacgg gatcacgcca
     9961 ttatttcgtt cttcgatcag gggatgaatc ctgcagtgcc cagggttgcc tactggaccc
    10021 cctttcgtta cccgctgcta ctgaatctgg caagcctgtt cgataatgaa ctggctgaaa
    10081 aagcgtggtg tgcccgcctc gaagcccatg atggaaggtc ctcatctctg ttcagtgagg
    10141 tctgcagtga gctgcttgcg cgcgttcata ccctggggga ccgccgttac attgaattga
    10201 ttaccgatgc gttgtcctgg gcgatgaccc actttgacga actgggatat aactgcaaaa
    10261 ccggtaaaca aaagctgcag atcatgccca atatggttgg atttcagttc gtattgcgcg
    10321 gtatctgttc ccgcctggga gcaccaaatc gcaaagccga catcgttgtg gaccagcagt
    10381 cacagttcaa caccacccag cgggagctga gggagttcta ttaccagata cgtgagatgc
    10441 cgtgggtaca cggtcccggt ttaccggtca tggatgtgac gaacatgccg gctgaacgct
    10501 ggttttccag tcgggtacaa aaagtgcagg gctggagctg gttgatatat atctctggat
    10561 cttcaaacgc tttatggaag ggaaggagtt aaccagaccc ccttacccgc ctggtttaca
    10621 ccaatcgcaa taccggcagg acggacagtg tctcccttca gtcggtggct aaacgatcta
    10681 aggagtttct cgacaaactg caggagccaa cggcagaaat gatgaaaaag gcgagggaat
    10741 accgggatca ggaagaggca aggcggctgg aacatcgggt gcaaatcctg ccgccatcct
    10801 gatccggcag gttgagtaca cacaaaaatg gaagcggcca cgacgggatg atgcgtctgt
    10861 ccggcatctt ccggagggcc agaactcgaa attctggccg tacttcactt cttcgttgaa
    10921 gttctatctg ttcgctgtga gtctcatatt tttattaaaa ctaaagcaac tcatcaacat
    10981 aagtcatgat ggaacgaacc agatttatgg caaaacgggc atcggcttca ttcagcaaag
    11041 tttcagtagg atgggccatg ctgtaattgt tacgcaggtt attgatccca tgaagtattg
    11101 cggcagatga tctcaacatt ttaaacacat cttcatttct tccatcatca ggcaggtctt
    11161 tgagatgtgc agttatcagg gaaagcaagc ctggaacatt atcatttgta cggtgatgct
    11221 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctcc
//
LOCUS       DAOFSV010000142        11069 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.142, whole genome shotgun sequence.
ACCESSION   DAOFSV010000142 DAOFSV010000000
VERSION     DAOFSV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.142"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            177..938
                     /gene="adiY"
                     /locus_tag="QBH27_003844"
     CDS             177..938
                     /gene="adiY"
                     /locus_tag="QBH27_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDQ7016102.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1075..2412
                     /gene="adiC"
                     /locus_tag="QBH27_003845"
     CDS             1075..2412
                     /gene="adiC"
                     /locus_tag="QBH27_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDQ7016103.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2516..4159
                     /gene="eptA"
                     /locus_tag="QBH27_003846"
     CDS             2516..4159
                     /gene="eptA"
                     /locus_tag="QBH27_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDQ7016104.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4156..4824
                     /gene="basR"
                     /locus_tag="QBH27_003847"
     CDS             4156..4824
                     /gene="basR"
                     /locus_tag="QBH27_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDQ7016105.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4825..5925
                     /gene="pmrB"
                     /locus_tag="QBH27_003848"
     CDS             4825..5925
                     /gene="pmrB"
                     /locus_tag="QBH27_003848"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDQ7016106.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5901..5990)
                     /gene="pmrR"
                     /locus_tag="QBH27_003849"
     CDS             complement(5901..5990)
                     /gene="pmrR"
                     /locus_tag="QBH27_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDQ7016107.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6102..7604)
                     /gene="proP"
                     /locus_tag="QBH27_003850"
     CDS             complement(6102..7604)
                     /gene="proP"
                     /locus_tag="QBH27_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDQ7016108.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7868..8746)
                     /locus_tag="QBH27_003851"
     CDS             complement(7868..8746)
                     /locus_tag="QBH27_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDQ7016109.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8743..10971)
                     /gene="crfC"
                     /locus_tag="QBH27_003852"
     CDS             complement(8743..10971)
                     /gene="crfC"
                     /locus_tag="QBH27_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDQ7016110.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     NSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2789 a         2881 c         2678 g         2721 t
ORIGIN      
        1 ttttcttagc ctgtaaaaaa atatgtacat gagaaattac tataaaaatt tgtaatatta
       61 gtaaaactcg ttatttttat gcatgtttat attcatcata caattatata accatttccc
      121 ggtatcgctt cgctttagcg agaactggtc ttttattcgc actcaggagt acatgtatga
      181 ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc tggataaggg
      241 tgaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta aattgtgaaa
      301 ataatattat cgacgtctcc tctcttaata acactttggt tgctcatatt agtcacgaca
      361 tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca gtatggcaac
      421 gtagcgctac gcccatcctc acccttccat gcctgacgcc agacgtcttt cgcgttgccg
      481 cgcaacatag catgatgccc gctgaaactg agtcagaaaa ggaacgaaca cgtgcattat
      541 tattcactgt gctatcccgt tttctcgaca gtaaaaaatt cctttcacta atgatgtata
      601 tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat atccacaaag
      661 actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg ttaaagaaaa
      721 agctgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc atgcgctatg
      781 ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag tcctgcggct
      841 acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg acgcctctgc
      901 attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta acgaagagct
      961 atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc gaatgcaggc
     1021 gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt aattatgtct
     1081 tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt gtcggggaat
     1141 attatggggt caggtgtttt tctgttacct gcaaacctgg cctccactgg cgggattgcc
     1201 atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt atacgccaaa
     1261 atgtcgttcc tcgacccaag tcctggtggt tcttacgctt acgcccgccg ctgctttggg
     1321 ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat cggcaatatc
     1381 gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct gaaagatcca
     1441 ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct gaacattgtc
     1501 ggtccgaaaa tgatcacccg cgtacaggcc gttgctaccg tgctggcgct gatccccatc
     1561 gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat ggcggcatgg
     1621 aacgtcagcg gcctgggcac cttcggtgcg attcaaagta cccttaacgt tacgctgtgg
     1681 tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa cccgaaacgc
     1741 aacgtcccta tcgccaccat tggcggggta ttgattgctg ccgtttgcta tgtactttct
     1801 accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc ttcgccattc
     1861 ggtgatgccg cacggatggc gttgggtgac accgccgggg ccattgtttc cttctgcgca
     1921 gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcaggtca aacggcgaaa
     1981 gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa agcgggcacg
     2041 ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct cagtagcatt
     2101 tcgccaaacg cgaccaaaga attcggtctg gtttcttccg tgtcggtcat ctttacactg
     2161 gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gccacggtca ctttggtaaa
     2221 gcgcgcccgg catacctggc agttactacc attgccttcc tctactgcat ctgggccgta
     2281 gtagggtccg gcgcgaaaga ggttatgtgg tcatttgtca ctctgatggt aatcaccgcc
     2341 atgtatgcgc tgaattacaa ccggctgcat aaaaacccgt atcccttaga tgcaccaata
     2401 agcaaagatt aattcccctt aatccagcaa acataaaagc caaccttaag aacttaaggt
     2461 tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca ccgtgatgtt
     2521 gaagcgccta ctaaaaagac cctctttgaa tttattctcc tggttattac ttgctacttt
     2581 ttatatctca gtctgcctga atatcgcctt ttttaaacag gtgctacagg tgttaccgct
     2641 ggattcactg cataacgtgc tggttttctt gtcgatgccg gtcgtcgcat tcagcgtgat
     2701 taatattgtc ctgacactag gctctttctt atggcttaat cgtccactcg cctgcctgtt
     2761 tattctggtc ggtgcggctg cgcaatattt catcatgacc tatggcatcg tcatcgaccg
     2821 ttcaatgatc accaatatca ttgatactac gccggcggaa agttatgccc tgatgacgcc
     2881 acgaatgtta ttaacgctgg ggttaagtgg cgtattagcc gcgattattg cctgctggat
     2941 caaaatcaaa cccaccacct cacgcctgcg cagcgttctt ttccgcgggg ccaacatcct
     3001 gatttctgta ctgctgattc tgctggttgc cgcactgttc tacaaagact atgcctcgct
     3061 gtttcgcaac aacaaagaac tggtgaaatc cttaagcccg tccaacagca ttgttgctag
     3121 ctggtcgtgg tactctcacc agcggtcagc caacctgcca ctgatacgca ttggtgaaga
     3181 tgctcaccgc aacccattaa tgcaaaatgg gaaacgtaaa aacctgacca ttttgatcgt
     3241 cggagaaacg tcgcgcgcag agaatttctc gctgaatggt tatccacgcg aaaccaatcc
     3301 tcgtctggca aaagataacg tggtctattt ccctaatacc gcctcttgcg gcacggcaac
     3361 ggcagtttca gtaccatgca tgttctcgga tatgccgcgt gagcactaca aagaagagct
     3421 ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca acgtgctgtg
     3481 gaatgacaac gatggcggct gtaaaggcgt ttgcgatcgc gtacctcacc agaacgtcac
     3541 cgcgctgaac ctgcctggtc agtgcatcaa cggcgaatgc tatgatgaag tgctgttcca
     3601 cgggctggaa gattacatca ataacctgca gggtgatggc gtgattgtct tacacaccat
     3661 cggcagccac ggcccgacct attacaaccg ctatccgccg cagttcagga aatttacccc
     3721 aacctgcgac actaacgaga tccagacctg tacccaagag caactggtga acacttacga
     3781 caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc tgaaagaaca
     3841 tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat cgttaggtga
     3901 aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc aaaaacaggt
     3961 gccgatgctg ctgtggctat cggaggatta tcaaaaacgg tatcaggttg accagaactg
     4021 cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct caaccttatt
     4081 gggcctgact ggcgttgaga cgaagtatta ccaggctgcg gatgatattc tgcaaacttg
     4141 caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt attgcaggga
     4201 ttgattctgg cggcgcaaac cgaaggctac gcgtgcgatg gcgtgagcac cgcgcggatg
     4261 gcggaacaaa gccttgaagc cggtcattac agcctggtgg tactggattt aggcttaccc
     4321 gatgaagatg ggctgcattt tcttgcccgt atccggcaga aaaaatacac tctgccggtg
     4381 ttaattctca ccgcccgcga tacccttacc gacaaaatcg ccgggctgga tgtcggggcc
     4441 gacgattatc tggtgaagcc ttttgcgctg gaagagttgc acgcccgtat ccgcgccctg
     4501 ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaacct gacgctgaat
     4561 atgggtcgcc gtcaggtctg gatgggcggt gaagagttga ttctgacgcc caaagaatat
     4621 gctctgctgt cgcgcctgat gcttaaagca ggcagcccgg tgcatcggga aattctctac
     4681 aacgatatct acaactggga caacgagccc tcgaccaaca ccctggaagt gcatatccat
     4741 aacctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggatt tggctatatg
     4801 ctggtcgcaa atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg accaatatcg
     4861 ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga gctgatcagc
     4921 gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca ggcgctacgc
     4981 gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt cgccagcctg
     5041 attgtccccg gcgtctttat ggttagcctg acgctattta tctgctatca ggcggtacgc
     5101 cgcatcaccc gaccgctggc ggagctgcaa aaagagctgg aagcgcgcac cgccgacaac
     5161 ttaacgccca ttgccattca tagcgccacc ctcgaaatcg atgcggtggt ttcggcgtta
     5221 aacgatctgg tcagtcgcct gaccagcacg ctcgataatg aaaggttgtt taccgctgac
     5281 gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga actgctggcg
     5341 aaaacgcatc acattgatgt agcaccgtta gtggcacgac ttgatcagat gatggagagc
     5401 gtctcccagt tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg taattatcaa
     5461 catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag taccatgctc
     5521 gaccagcgcc agcaaaccct gctattgcca gagagcgccg ccgacatcac cgttcagggt
     5581 gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca tcgttatagc
     5641 ccgcaaggca gcaacattat gattaagctg caagaagatg gcggagcggt catggccgtt
     5701 gaagatgaag gaccgggtat tgatgagagt aaatgcgggg agttgagtaa agcatttgta
     5761 cgaatggaca gccgttatgg cggaattggt ctggggttaa gtattgtcag ccgcattaca
     5821 cagttgcatc acgggcagtt tttcctgcaa aaccgacaag agacttccgg cacgcgggcc
     5881 tggatacggc tgaaaaaaga tcagtacgtg gcaaaccaga tataaagaaa gctgctgacc
     5941 accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat tgcactctcc
     6001 tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa gagttcaatt
     6061 gacctgataa gacagcatca catcaggcca tccgtttcag cttattcatc aattcgcgga
     6121 tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg gtcaatatca
     6181 tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc ttcctgtata
     6241 tctgacgccg ccggtgtcgc acctttcaac ggacgatttg ccgtctcttt catggttacg
     6301 ccggtgatta aaccaaccac cgccactacc atcaggtaat aggcaggcat catcagattc
     6361 tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac cagcaccgaa
     6421 atattaaatg ccgccgccag cgcgctgtat cggatatgcg tcgggaacat cgctggcaag
     6481 gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag taacccggca
     6541 aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc caacacaaac
     6601 agtgcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact cagcaagccc
     6661 atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag caccccgtgg
     6721 tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag caacatgtag
     6781 taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg ccagtgtttg
     6841 gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg gtcgccctgt
     6901 tccagtttat cgacatgctg ctggaacgcc ggagtctctt ccagcgcatg gcgcaggtaa
     6961 agcccgataa tccctaacgg cagagcgata aagaacggaa tacgccagcc ccaatcgagg
     7021 aagttctctt cgccgacaat ggtcgaaatt aacaccacta cgccagctcc cagcacaaac
     7081 ccggcaatag aaccaaagtc cagccagctg cccataaagc cacgtttacg gtcaggggag
     7141 tattccgcaa caaatattga cgccccggta tattcaccgc cgaccgagaa accttgtgcc
     7201 atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta ggacggtatt
     7261 aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag gatcttctgg
     7321 cgaccatatt tatcgcccaa cataccaaag aagagcccgc caagcggtcg aatcagaaag
     7381 ggaacggaga aagtggcgag tgcagcaacc atctgcacgc tggggtcagc ccccgggaaa
     7441 aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcgaa ccattccatt
     7501 gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccatcatc aataatggtg
     7561 acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt cctcgcagag
     7621 ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag gggagcggga
     7681 attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct ccggttaagg
     7741 aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt ttaccctacc
     7801 agtgtttaaa tttgtgatca acttcacaca tgcatttcgt ttaggactca ttggtgtaac
     7861 tgattattta caccaaattt acagagaaga tttcctcgac cattgccccc gccagacgct
     7921 gcgctgaaca gagtcgtggc atccccagtg cgacagttcg ccagcgttgg gtcactgacc
     7981 agcaaacggg ttgtttatct gccgtagacc actttcccag ccaacttaaa aaatcagcat
     8041 gatccccgcc cgtcgaaacg gtcgaagagg caaaccactg atggtaatag tgacgcgtgg
     8101 tcggggccgc attgatactc tctgccaaat gttgtgtcgc tttcacgcgc aaatcaccta
     8161 gcaacaacgc caccgcttgc gggcttccca gacggcaggc atcgccaaat gcaccccagc
     8221 gcaactcttc gagaacgata aaacagcgcc caggcaacga ccaggcgtta tacgccccag
     8281 cacgccagcg ggtaaaaatt tgctcgctct gttcacccgc ctgcaccaat aggcctcgtt
     8341 gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac aaggcgacaa
     8401 tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt tgcagcagcg
     8461 ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg atccagcatc gacgccagtt
     8521 tttccaccaa tgtcagacgc aggctaagcg gcgcatttaa catgccgccg ttcgtccatg
     8581 tctgaagctg cacgcgcgca agaagttcct gcatcagtcg ttcacgaaac tgctgctgat
     8641 gggcttgtgg aaggcgggca tcatcgccct gcgccagatc cactaaaaag cgcggatgaa
     8701 ccgactccag cacgcgaccg gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt
     8761 gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg gtgtaaatcc agccgtgagt
     8821 catcacagtc tgctgcaatt gctgttgcat cagcctgacc accgattcat tttgttgacg
     8881 cagagccata ctttcgcgta aacgcgtctg taattccgtc aaacatgaag cgaactcagc
     8941 gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca taactttagc
     9001 catttgttgg caataaagat cgacttctgc gcttaatgct cgttgcaaca cactgtaatc
     9061 aaccgtctct gtcgggaatt tctcatttcc ccgtccccag tcgggctgat tcaaccagcg
     9121 cgaaaaaatc tcacgcacaa cgcctaaacg cgtgctctgc tcgtccgttg cttcctggcg
     9181 cgaaatgact tcactgaaca gctggcgggt gttgaagtgg ggaactacgc cgtgaaaaac
     9241 aggaaaatga aacccaggac gaaacccaga ctcactcaat tccattttga cttgttgctc
     9301 aatggggcga ataacatcgg ttaacacccg gcaaagggtg gactcaagct cggcaaagcg
     9361 cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct cacagcgggt
     9421 acgcatctca cttaacgact ccgaattatc ctgaaacaac gcggctaact gcgcattcac
     9481 cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc agggccaact cgatttcatg
     9541 tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac tttcttcgac
     9601 ctgatggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg cacgaaaatc
     9661 caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcggcag atcgcagagc
     9721 atacaacgag gcgttagcgt aagcggcatg aagcaacgcc tgaattggct gggcgaacag
     9781 cgaatcttcc cacaactgat cggcagcatg gcgaatatgt tcgaggtccg ccagatcggc
     9841 atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc gttgttgctc
     9901 tggcgcgggt aacttaccgt tgttggctaa ctcatggcgc gcccgattcg ccaggtagcc
     9961 ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca tcagcgttcc
    10021 ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt
    10081 attgaccagc acatacaacg gcaccgattg ccccaccgcc aaaattgcct cacggacctc
    10141 ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg ccgaggcgcg
    10201 tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat ttggcccggg
    10261 ggtatccagt aacgtcaact gaccgggata actctccagc cccgccagat ggacaaactc
    10321 cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc
    10381 cagcgccttc gccagtcgca ccaaatcatt caaacttttc agacaatgaa aaataggttg
    10441 agcacccaga taatattttt cgaaagcgat gccattttcg atccgctgca taagcgcacg
    10501 catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat cacgcaggcg
    10561 ctgttgtaat tgttgaatta aatggtcgat tggcgcgaca tgtgaaaaat gcagtaccgg
    10621 ttctttttgc ccgggcgtat ggcgaataag cgttggcagc gcagtcattg ggcgattgcg
    10681 attaggcaga acctccgtac caacaatggc attaatggtg gttgatttcc ctgctttcat
    10741 ggtaccgaca atcgccagca ccatttccag ccgggaaatt ttacgcaact cattattcag
    10801 catcgcgtga cgttcggcga tattaggctg actccagggt aaagccagtt gtggcgcatc
    10861 gtctccgggt acagagagag gtattttttc cagtaactgc aactgttgtc gggaaagctg
    10921 taacaggcgt tcagcctcct gacttaactc atacagggtc tgtgtgtaca tagaatattc
    10981 ttccttaaag caaattttat tacttttatt tagccagatt gtttttgagt tctgttttcg
    11041 gcttttataa ttactgcagg aaataattt
//
LOCUS       DAOFSV010000143        10901 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.143, whole genome shotgun sequence.
ACCESSION   DAOFSV010000143 DAOFSV010000000
VERSION     DAOFSV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.143"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(37..1701)
                     /gene="glnS"
                     /locus_tag="QBH27_003853"
     CDS             complement(37..1701)
                     /gene="glnS"
                     /locus_tag="QBH27_003853"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDQ7016111.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1921..2682)
                     /locus_tag="QBH27_003854"
     CDS             complement(1921..2682)
                     /locus_tag="QBH27_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7016112.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2684..3670)
                     /locus_tag="QBH27_003855"
     CDS             complement(2684..3670)
                     /locus_tag="QBH27_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HDQ7016113.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTEKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3663..4898)
                     /locus_tag="QBH27_003856"
     CDS             complement(3663..4898)
                     /locus_tag="QBH27_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDQ7016114.1"
                     /translation="MEKGITKPSILIVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     CAMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKRSE"
     gene            5068..6225
                     /locus_tag="QBH27_003857"
     CDS             5068..6225
                     /locus_tag="QBH27_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDQ7016115.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFAQVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6229..7116
                     /locus_tag="QBH27_003858"
     CDS             6229..7116
                     /locus_tag="QBH27_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDQ7016116.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADTLSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSDGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANVAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7176..7934
                     /locus_tag="QBH27_003859"
     CDS             7176..7934
                     /locus_tag="QBH27_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ7016117.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            7970..9139
                     /locus_tag="QBH27_003860"
     CDS             7970..9139
                     /locus_tag="QBH27_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDQ7016118.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAAKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWSWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9136..9606
                     /locus_tag="QBH27_003861"
     CDS             9136..9606
                     /locus_tag="QBH27_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ7016119.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCSLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9620..10900
                     /locus_tag="QBH27_003862"
     CDS             9620..10900
                     /locus_tag="QBH27_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDQ7016120.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQAISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
BASE COUNT         2511 a         2875 c         2801 g         2714 t
ORIGIN      
        1 ggatgcggcg taaacgctct atccgacaac gtaaacttac tcgcctactt tcgcccaggt
       61 atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gccgtagaat ggcggctgtc
      121 gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgcaaccg catctttcag
      181 cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa
      241 atcatccgca gcaccagggt tcggcacgct gaacagacgg tcatacaaac ggatttcaac
      301 cggcagcgca tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc
      361 cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga tattgccttc
      421 ggcatctttc tccacgcgtt ctgccttaat gacgtaagca ttacgcagac gcacttcttt
      481 acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat
      541 ccaaatctca ccgctaaatg gcacctgacg gctgcccatt tccggtttat tcggatggtt
      601 cggcatggta accatttcgc cttcgccctg gtagttttcg ataaccagtt tcaccggatc
      661 gataaccgcc attgcgcgcg gcgcattttc gttgagatct tcacggatgc aggattccag
      721 tgacgccatc tcaatggtgt tgtcctgctt ggtcacacca atgcgtttgc agaactcacg
      781 aatagaagcc gcagtgtaac cacgacgacg cagaccagaa atggtcggca tacgcgggtc
      841 atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac
      901 agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat
      961 gttgtccagt acccagtcgt acagacgacg gttgtcctgg aactcaagcg tacacagaga
     1021 gtgcgtaata ccttccagcg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat
     1081 gcaccacttg ttgccagtct ggtggtgttc agcaaactta atgcggtaca gcaccggatc
     1141 gcgcatcacg ataaacggcg aagccatgtc gattttcgca cgcaggcagg ctttaccttc
     1201 ttcaaaaccg ccggtacgca ttttctcgaa cagcgccagg ttctcttcaa cgctgcgatc
     1261 gcggtacggg ctgtttttac ccggctgcgt caaggtgccg cggtattcgc ggatctgttc
     1321 cggcgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg
     1381 gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca
     1441 ctctacgtcg tttttgatcg aatcgacata ctcgatatct tcttttaccg ggttagtgtc
     1501 gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat
     1561 agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac
     1621 tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg
     1681 gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta
     1741 tgatcttata agcgggacag gctgacaact gttagttagc gaaaaaatgg ttttgcgtaa
     1801 gatattgggg cgttttctgc gctactgcca gcagatcctc gcgctggaaa cgtaaggcca
     1861 aacgtcgcca gcctgacgca tttattaaat ttccggatga gctggccgca gagtcgtaga
     1921 ttagtcggct atgattaaac gcgtcgacaa ggacgcaagc gttctacgcg tctgcccatc
     1981 aagctgacta tccgtaataa tctctgtcag ttcattaaga tgcgcgatat taaacagcga
     2041 ccatgaaccg tattttgagc tgtcagccag gagaattttg cgcgaagcat gcgcgataag
     2101 atcgcgtttt agtgcggctt tctcttccgt gggtgctgta ataccctttt caagatccca
     2161 gctgttacag cttaaaaatg ccacgtccgg ccaggtattt tgcagcaatt tacgaccatg
     2221 ttcgccaatg caggactggc tggagtcgtc aatacgtccg ccgataatcg tcacttcaat
     2281 ttgcttgaat tcagccaaaa aaagcgcgat atgcaggtcg cttgtgatca cgcgaagcgg
     2341 taaatgtgta atctgccgcg caagttcaat cattgtcgta ccggcatcaa ggacgacgga
     2401 atcccccgct ttgaccattg acgccgctgc gctggcgatg gcatgttttt ccgccaggct
     2461 gcgctgcatt ttctcatggg tagtcggttg ggtaggaata aaccggttga gagtcacacc
     2521 gccgtgtgta cggctgataa caccctgctc atcgagtttt atcaggtctc gacgaatcgt
     2581 agccggcgac gcattggtta ccgccaccag ctcatctacg gtgaccagat tatggctttt
     2641 caggtaatcc atgatctgct ctaaccggtt atatcccttc attttattct cgcgaaatct
     2701 gcattgctaa ctggatggaa actgccatgc tctcagactt ggcttttcct gtccaggcaa
     2761 tatcaaacgc ggtgccgtgg tcggcagaag tgcgaataaa tggcaacccg gcggtgatat
     2821 tcaccccatc ataaaatcca agcagtttta aagggatatg cccctgatcg tggtacatag
     2881 ccactaccat atcgaatagc ccttcatggc attgcataaa cacggtatct ggcgggcacg
     2941 ggccagtaac atcaatatct tcggcctgca ttgcttcaat cgcaggcgcg ataatctcga
     3001 tttcctcagt gccgaataaa ccatgttccc ctgcatgtgg atttacaccc gccacggcaa
     3061 tacgcggacg ctcaatacca acacgtttca ggaagtggtt cgcaactcga ataaccgttt
     3121 tgaccctttc accattcaaa gtatccagga atttacgcag tgagatgtga gtcgaaatat
     3181 ggataacctt gagcttctcg gtatagagca ccatcgcgta ctctttgctg ccggtaagat
     3241 gggccagcaa ttccgtatgc cccggatagt tatgcccgcc aagatgcagc gcttctttat
     3301 tcagcggcgc agtggctatg gctttcacct cgcctgataa ggccagagcc gttgcacgtt
     3361 tgatacagcg atacgctaaa tcgcctgcca cagcctgtac cactccgggt gtcagcgcat
     3421 cggggttagc cagcggctca tccatcacgt taatcacgcc gggagcaaaa tgggcgtcgc
     3481 acaccttttg aatgacgcgt aactctgcct gcggcgtgat attcatcgcc ataatacgtc
     3541 gcagcgtgcg cacacagccc accaccacca ccggggcacc agcaagcccg gcttcgctca
     3601 gggatttaat aatgatctcc gggccaatac ccgccggatc gcccatcgtt accgcaataa
     3661 tattattcac tacgcttctc ctcgataaaa ttcagaacct gatgcaacgt ggtctcatcg
     3721 ccaaacccac cggctttcgt catcaccgaa cgggaccaga cgccgcccag aaagtgaccg
     3781 tatggaacgc attgcgccac cttccccgta atacgaaatc cggtcgcgcc taacgcactg
     3841 gctgttgcca ttgcgacatc accaccagag agataaagcg catcgggcat tgcgcaaagc
     3901 agaacttgcc gggtaagttc gccaagaaac cggcaaattt tttccccaag tgcagcgcga
     3961 ctcatctgcc aacgctggca aagggtgtcg atctgatgcc ttgctacaga atcgttacag
     4021 gtgtggacaa tacagtggtc gcctttctgc aaggcctgca caatacgcgc gtcatagtca
     4081 ctggcattgc ctgccagaat gtgttcaaca tcgacgtata tctgtgtgat acgtgggtgt
     4141 gaatgaagtg tggcgatctg ccgttgagcg atttcgctca ttgaaccaat aatggccagc
     4201 acggtgggtg gcggcataat cgcggagcgc cgggcaacgg catcacaaat accagcggag
     4261 ccgaccagca gcagcggttg tttagcctgc atcacagcgg cagcgatgcg atccaggtcg
     4321 ctgtccgtct gcgcatctat cacgccaatc cacggttgct cataagacaa atggctgagc
     4381 tgagaaaccg taacgggctg gcatggaatg gccgtctgca accggatgat gtccgcaatt
     4441 cgggcgctca ataccggggt tttcggatcg ctagcaaact cggtttccgt tatccgcaca
     4501 ccgtttaccc aacactcacc ggccactgtg gtccttcccg cctgaggaaa tgcgggggca
     4561 accactgcgc ccgttatacc gcatgccttc atcagggctt caatttctgc gccgatattg
     4621 cctcgcagcg tggaatctat ctttttaatc acgagcggcg ggttattcgc cagagggaga
     4681 tgactcatca gcgaggttat tttcatcgcc gccagcttag gatccatcgc ccgactgtcg
     4741 ctgttaatca cccatacgct ggcatcaccg cgataaggca tttcaaaggc cacatcaact
     4801 gtatgcccca cctgcgccag gcttaccccc gcgtcgttgg ctcccgtgaa atcatcggca
     4861 acgatgagaa tgctcggctt agtgattccc ttttccatca tcactccagg ttgttttatg
     4921 ataacaatga ttatattcaa tcatgattga ttaaatgtga gcaagctcaa gttattaatc
     4981 actcgaataa attataaaat tagaccatgt tacgtaggct acggcatttt gaatgattga
     5041 attcaatcac cagaagagag gttatttgtg gtaattatca atagcacgat actgagcggc
     5101 gcaggcgcta tcccttccct gacgtcgctc ttacccgaca tcagaaaaat gctgctggtc
     5161 actgaccgta atattgcgca gctcgacggt gtgcagcaga ttcgcgcctt actggaaaag
     5221 cactgcccgc aggttaacgt tatcgataat gtgcccgcag agcccacgca tcatgatgtg
     5281 cgccagctaa tggatgccct tggcgatgcc tcttttgatg tggtggtcgg gatcggcggt
     5341 ggcagcgtgt tggatgtggc gaagctgcta tcggtgcttt gccatccaca atcaccgggg
     5401 ctggatgcgc tgcttgcggg tgaaaaaccg actcagcggg tgcaatcatg gttgattcct
     5461 acaaccgccg gaaccggctc agaagccacg ccgaatgcga ttctggcaat ccctgagcaa
     5521 agcacgaagg tgggtattat ttcccaggtg ctgttaccag actatgtggc gcttttcccg
     5581 gaactgacca ccagtatgcc cgcgcatatt gcggcgtcca cgggcattga tgctctttgc
     5641 cacttactgg agtgttttac cgcgaccgtg gcaaatccgg tcagcgataa cgcggcgctg
     5701 actgggttaa gtaaactttt ccggcacatt caacccgccg tgaacgatcc tcaggatctg
     5761 cgcgcaaaac tggaaatgct gtgggcgtct tactatggcg gcgtagcgat aacccatgcg
     5821 ggcacgcatc tcgttcatgc gctctcctac ccgttaggtg gcaaatatca tctgccgcat
     5881 ggcgtcgcga atgccatctt gctggcgccg tgcatggcgt ttgttcgccc ctgggcggtc
     5941 gagaaatttg cccaggtctg ggattgcatt cccgatgcgg aaaccgccct gagcgcggaa
     6001 gaaaaatctc atgccctggt gacctggtta caggcattag tcaatcaact caagctaccc
     6061 aacaatctcg cggatctcgg cgtaccgcca gaggatattg cctctctgag cgaggcggca
     6121 ctgaacgtga agcgccttat gaacaatgtg ccgtgccagc ttgatctaca ggacgtacag
     6181 gccatttacc aaacactgtt tccgcaacat caatttaagg aataaatcat gccgaaaacc
     6241 attgaaggtg tattaaccgc tatcgtcacc ccgtttaccg cgaccggcag tctgaatatc
     6301 ccggcgttaa aagtgcaggt gaatcgtcaa ctggaagcgg gtaacgcgat tttttgcggc
     6361 ggcaccaacg gcgagttttt tgtgttgaat gaacaggaga agctcagcgt tacgcaaacc
     6421 tgcgtggatg aagtggctgg tcgtgcgcca gtcgtggcac acattggcga gatctctacg
     6481 cgcgaaacga ttcgtctggg tcagcagatt gaaaaacttg gcgtagatgc cgtgtcggtc
     6541 attgcccctt acttcgtacc gctgaaacag gaagagttga ttgcgcatta cagcgcaatt
     6601 gccgacacac tgagcattcc gatgtttctc tataacatcc cggcccgcac tggcaatacg
     6661 atccagccag aaaccgcgcg tgctctggcg tcgcacccca atatcattgg gattaaagac
     6721 agcgacggaa gcgacgaaag cctgaagggt tttctcgatg cggtgcgcga cattgacggc
     6781 tttaatgtgc tcaacggccc ggattcgctg atccataaag ggtttgtcga gggatgttca
     6841 gcctgtatct ctgggctcgc caacgtcgcg ccgcgagaaa tcaatgccat ctggtctcgc
     6901 ttccatgccg gtgatattga agggtcttat gaagcacagg agagtgtaac gggcctgcgt
     6961 accgatcttt ataaagtagc attctccccg gctgcggtga aaaaagcgtt gcagcttatg
     7021 gggcatgatg ttggtgatag ccgctacgcg gttaatttca cgcctgaaca gatcagcgag
     7081 attcaacaaa ttattcgtca ttataacatt aattaaatta actctattat ccagcccgct
     7141 ataaagttta gataaagaga ataaaggatt accccatgtt tttgtggttt atgcagccag
     7201 atatctggct cactttaatt accctcacct ttctggaaat tattcttggg gtggataata
     7261 ttatttttct ttcactggtg gtggcaaaac ttccgttatc acaacaaaat atggcgcgta
     7321 aactgggttt aagctgcgcg atgattatgc ggatattatt gctgatatcc attgcgtggc
     7381 tttcacatat tacccagccg ctttttaccg tgctgacgct ggagatttcg ttccgcacgt
     7441 tgattctgct cgggggcggg ctgttcctga tttataaagc gatacaggag atacgcgaag
     7501 agctgtcgcc cgcgcaggaa gacgaacatg gcagcggatc gcggcaactg tcgttgtgcg
     7561 gtgccattgt acagataatg ctgctcgata tcgtgtttag cctggattcg gtgatcaccg
     7621 ccgtcggcct ttctcagcat atatttatca tgattgccgc cgtaatgatt gccgtcggtg
     7681 tcatgatgtt tgcggcgaaa acaattggtg atttcgttaa cgccacgccg tcaataaaaa
     7741 tactggcgtt aaccttttta ttgtttgtcg gtgtattgct ggttgccgat agtctcaaca
     7801 ttcatattgc caaagagtat ctctacttcg cgatattttt ctcattaagt gttgagacac
     7861 tcaatattat ccgtgaaaga aaaaataaaa aagtttcgac attgatcgaa acgcatgaat
     7921 cgcactcgca ttaataaatc tattaccaac tttaagatgg agtctaacta tgtctgtagc
     7981 ccttattcgt gacggcgtca tccgtccatc cgaaaacgat tcgcatgtgc ttaccgccat
     8041 gctgccctcg tcctgcccgc aaaaccacgc ggcgaatatt ctgcctttgc cggatggcgc
     8101 gttaatgtgc gtctggtttg cgggaacgca ggaaggtatt gccgatattt ctgtctgggg
     8161 atcgcgattg cccgctggcg gaatgcagtg gagcgatgcg gccaaacttt cccacgatga
     8221 cacgcgttca gagcaaaacc cggtgctgtt tctcgcgccg gataacgtgc tgtggctcct
     8281 atggaccgca caaatatccg gtaatcagga caccgccatc gtccgctacc gtaagtcaga
     8341 cgacctcgga caaacctggg gcgagattgc caccctgctt gataaacccg gtacgttcat
     8401 tcgacagcca atcacggtgc ttgataacgg caactggctg ctgcccgtct tttattgccg
     8461 tacccaaccc ggcgaaaaat gggtcggcaa tgatgatatc agcgcagtga aaatttccgc
     8521 tgacggtggt cactcctggc gcgatgtcga ggtgccgcaa agtctcggct gtgttcatat
     8581 gaatatcaca atgttgcacg acggtacgtt ggttgctctc tttcgcagcc gttgggcaga
     8641 caacatctat atcagccatt cagtggacaa cggcgagagt tggtcggtgc cgcaagcaac
     8701 cgaattgccc aacaacaatt cgtctattca ggtaacgacg cttgccagcg gcgaactggc
     8761 gctggtgtac aacgcgatga gtgcggcagg cgcagtagaa cgtcgcgcgt cgctttatga
     8821 cgaaatcgac gatggcgacg acagccgcaa agaaccgaca gccgtcggac gctccgcttt
     8881 ctggggtgcc ccacgcgcgc cgatgacggt agcgatttct gccgatggtg ggaaaagctg
     8941 gtcgtggcgg cgcaatctcg atgaaggcga cggctactgc atgaccaaca attccctgga
     9001 aaagctaaat cgtgagttct cctaccccag catcaaacaa agcccggacg gtacgctaca
     9061 tatcgcttac acctggtggc gtcaggccat caagtacgta cgcatctccc ccgagtgggt
     9121 taaggggcag gcatcatgat tatcggtaat ctcaaccatt tgtcgctggc ggggcttcct
     9181 gcctgggtga gaaatattct gctgcgcccg gagtgttcgt tatcggcgtt atccaccaga
     9241 gaggacggtc gctggcaacc tgaaggatgt cggtggttct gctcgctggg aacttcagac
     9301 acgcaacccg ccgaacttcg ccatacggag tatcaccatc tatgggccga tatacaggtg
     9361 gtgattactg gttgtgaggg cattaatgcg ggaacgcggc ccatcgctcg cgaaaacgat
     9421 gaagaacgta agcccgatct gtttatcgcc ccatcccctg aaaacagtgt cgccatcacc
     9481 ctacacgccg gtgatttcgc cgtctttatg cctgaggaac ctcaccaggc actgtgcgca
     9541 attggcacgg ctacttcaat acgcaaagcg gtctttaaag taccgcgcga catgctggaa
     9601 gcctaaaagg agagaaaaaa tgacaatgga aatgatcctg gcattaggaa tactggtcct
     9661 gatgatcgtg ctgatcatgt cggacaaaat gccttttggc gcaccgccac tgcttgcgtg
     9721 tttattgctg gttgtatcgg gactttcgac cgttcaacag gcttttgccg gattcgtcaa
     9781 tccaagcgtg gtgatgattg cagggtttat ggtggtcatg gcggcgctac aaaaaacccg
     9841 gcttatcagc aacgttaaat ctgcgatgat aagcctggtc aataaaggca gttaccgcag
     9901 ctatgggctg ttgttagtca ttgttatgct gggtgccagt cttgccggca ccggagcgac
     9961 cggttattac gtgctaattc tgtcgctggt atccaccatt ccgtacagta aaaagctacc
    10021 aacatcgaaa ttaatgatgc ccctgggatt tgccaccaac cacccgcttc ttcctatcaa
    10081 tctcgcgctt ctctttggcg ttacggcgac cgtactcgaa accgcggggt ttcatcaggc
    10141 gatttcgatg gggcgctttg cgctggtgaa tctcatcatg tcagcggctt tcctcgcatg
    10201 gagtcttatt gcctaccgtt ttcttccgga tcatcccatt gcggatgctt ccgaggatgc
    10261 gctggcggca cgcgaggaaa cgtttaacgc ccttcctgcc tggaaagaat attgcactat
    10321 tgccgcattc gctgtcagcg ttatcggcat gatgctaatg aacattctgg gaaatatcgc
    10381 cttcgttatt ccgggtcttg cgggtgcgtt tgttctgatg atcgatgtac tggacttcaa
    10441 agaagttcgc gatcatatgg gggctccggt cattttaatg atggcggggg tgatcggtgt
    10501 agctgatgca ctggcgggca ccggctttac ggcgatggtt ggcgatgccg ttgcaggtgt
    10561 gctcggttct ggcgtctcac cgtttgtatt tattgtcgcc tttgctttgc ttaccagtac
    10621 ctgcgccacc ttcacgggtt cgaatatggg gtcagtatat atttttgccc ccatcgccat
    10681 tgccgcctgt actagccttg ggcttaaccc gaccgctgcc gcgattgcgg tggtgatttc
    10741 tggctggaat ggcggctata tgcctattga cggaatgcca gccatgatcc tcggaatggg
    10801 gaaatacaaa cttcccgaat tctgggtgtt ctctgtacca atgtatctca tccgcattct
    10861 ggcgctttgt gcaggcgctg tttttatctt cccaatgtaa t
//
LOCUS       DAOFSV010000144        10771 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.144, whole genome shotgun sequence.
ACCESSION   DAOFSV010000144 DAOFSV010000000
VERSION     DAOFSV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.144"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(148..1290)
                     /locus_tag="QBH27_003863"
     CDS             complement(148..1290)
                     /locus_tag="QBH27_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HDQ7016121.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(1280..1516)
                     /locus_tag="QBH27_003864"
     CDS             complement(1280..1516)
                     /locus_tag="QBH27_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDQ7016122.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(1656..1895)
                     /locus_tag="QBH27_003865"
     CDS             complement(1656..1895)
                     /locus_tag="QBH27_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ7016123.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
     gene            complement(1879..2205)
                     /locus_tag="QBH27_003866"
     CDS             complement(1879..2205)
                     /locus_tag="QBH27_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDQ7016124.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSD"
     gene            complement(2205..2426)
                     /locus_tag="QBH27_003867"
     CDS             complement(2205..2426)
                     /locus_tag="QBH27_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDQ7016125.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            complement(2525..2806)
                     /locus_tag="QBH27_003868"
     CDS             complement(2525..2806)
                     /locus_tag="QBH27_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDQ7016126.1"
                     /translation="MHFSGSGLHILCAYACRHGACSVTPQQENALRSIARQANSEIKK
                     VRQQFPDKNVNDICRSVLKKHRETVTLKGFTPTHLSLAIGMLNGVFKER"
     gene            complement(2817..3008)
                     /locus_tag="QBH27_003869"
     CDS             complement(2817..3008)
                     /locus_tag="QBH27_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDQ7016127.1"
                     /translation="MHKASPVELRTSIDLAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(2981..3163)
                     /locus_tag="QBH27_003870"
     CDS             complement(2981..3163)
                     /locus_tag="QBH27_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDQ7016128.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFHGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            complement(3160..3840)
                     /locus_tag="QBH27_003871"
     CDS             complement(3160..3840)
                     /locus_tag="QBH27_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDQ7016129.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(3837..4622)
                     /gene="bet"
                     /locus_tag="QBH27_003872"
     CDS             complement(3837..4622)
                     /gene="bet"
                     /locus_tag="QBH27_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDQ7016130.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(4628..4924)
                     /locus_tag="QBH27_003873"
     CDS             complement(4628..4924)
                     /locus_tag="QBH27_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDQ7016131.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHHVDIDSEV"
     gene            complement(5000..5206)
                     /locus_tag="QBH27_003874"
     CDS             complement(5000..5206)
                     /locus_tag="QBH27_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HDQ7016132.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            complement(5802..6491)
                     /locus_tag="QBH27_003875"
     CDS             complement(5802..6491)
                     /locus_tag="QBH27_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7016133.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            6596..6826
                     /locus_tag="QBH27_003876"
     CDS             6596..6826
                     /locus_tag="QBH27_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7016134.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            6896..7435
                     /locus_tag="QBH27_003877"
     CDS             6896..7435
                     /locus_tag="QBH27_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HDQ7016135.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYLEQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            7432..8451
                     /locus_tag="QBH27_003878"
     CDS             7432..8451
                     /locus_tag="QBH27_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ7016136.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            8448..9149
                     /locus_tag="QBH27_003879"
     CDS             8448..9149
                     /locus_tag="QBH27_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDQ7016137.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSDGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELACMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGAMK"
     gene            9399..>10771
                     /locus_tag="QBH27_003880"
     CDS             9399..>10771
                     /locus_tag="QBH27_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ7016138.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGN
                     KDVTQIYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNTHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKI
                     SGNGTSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVTDSGVSTADSSFT
                     LDGDSSPT"
BASE COUNT         2758 a         2473 c         2538 g         3002 t
ORIGIN      
        1 agagtttggt gacgcgatca tcaagaatat gtaatcacta catgtgttaa atattgaaac
       61 gggcgtataa cacgcccgtt gttttatgat gatgtaaaat cttccccaaa actttcccca
      121 aaacccttcc ccaaaactgg ctattttcta tgctgttttg atatctacga taatccagtc
      181 tttaccacga tcatcattgt atcggtcggt catttttctg gatttatggc ctaacaactt
      241 ttgcgtatcc agaccctgtt cccgatataa ccgttctgac agagatcgct gctcatgaaa
      301 tgtgggcgca gttccttgct cccattttat gccacatttt tcccgggcct ttttaaaagc
      361 cgttgtcaga gtatttgcag aaacctggtc tcctctgttt gcttgagagg tagtgtgacg
      421 gtaatggacc agatatttac taacaacagc atccctgcac tgagatataa cttcacgaag
      481 ggtaatattc agagcatcgc atttcaggtt aagcggaata gcaagttttg aaccggtttt
      541 ttcctgagta atgtgcaaca tgtcgtccca tatatcagag aatttcaaat tgcagatatc
      601 gcctaaacgt tgtccagtaa caagagcaag tagcatgccg cattttaaat agggctgccg
      661 tctgcttacg ctgtcaaata ttgcctgcca ttcgggcagt gacaatcttt ggcggtttac
      721 tcgatttcgc ggttgttttg ttgcctgcgc tgggttaaat cctggcggaa catgtcctgc
      781 gtgttgtgct tctttgaaga cgtcgatcaa caccattctc acgacttgcg ccatcctgtt
      841 atgaccttca gcctttacag catcaattat ttcggcaata tcaagtgcgg taatatcctt
      901 gaggtgttgc attccacaat gctcacggaa aagacgaatg ggtttgcctt tttgccgata
      961 ggagttgggt cttagttcat tatgttgcag cctgtcctct tggatagaaa tatatttatc
     1021 aagccattct gtcaccgtaa tgtctgagcg cctgcctttc attctttcca gacgctcatt
     1081 gacgcttaat atttgtctgg tacgttgttc agcaataatg gtatttgctt cagtagcaac
     1141 ttgttttgct tcattctcat cagttcctaa gctatgaaaa cgaccggata gtggatgttt
     1201 gtattgccaa tatacctttc cggttcgctt atctaatttg caatataaat tgggtataga
     1261 gattttgtga gatcggggtc tagcagccat cagcgattat cctttggagt tttgggtttg
     1321 cgtttattgg gagttgcggt tctgcaagcg ttcctacaaa acgggaattt cggtcaatca
     1381 tccagtagcg accaactttt atagcgggtg gggccatcat tttcccttgc gcgtattttt
     1441 tcagaactcg ctcacttggt gctaagtccc caaattcttc tttagcccag tcctgtaaag
     1501 tgattagtcg agacatttgt cctcctctta gctgctgagg gagtttgtga ccgatatatc
     1561 tgacatgata ttaagctcat ggcaggtaca tctcttgact ggtcatagag ataaatttaa
     1621 tgctgagaaa tgcagtattg aatttatcaa tttttctatt tcctgcgcat gtcacgtaac
     1681 ttcttaatgt gttctgccgt ttcgatctct tctgctatcc gatctgcatc agctttattc
     1741 acaggttcaa agtcatgatt aaagcggaac atgctggcga tacatgttct gccttttcgg
     1801 atgtagtgaa ctttgttgtg ggtagaacgc aggattttgc agggagtgcc gtggtggtcg
     1861 acgtaccagg tgttagggtc aatcactctg aacattcttg accttgtgtt gtgcggattg
     1921 atattcagtg ctgatattca cctttatagc gaacaccttt accggtttat cgccaaagtg
     1981 cggatgtgtg attgtcttca cttcatatcc gtcatacgga acatcaattc tgcgactgga
     2041 atcgtcgcgc ttcggatatc cctttgtgat aatcaggcgg tcatattccc ggaacataat
     2101 tcgcttattc cagtagtcat tacacaagcg atactcttcc gttttctctc cgcgaatcat
     2161 ggcatcgaag tattcacttt taacagcaag ttgcaggtta gccatcactt aatccccctt
     2221 tgtttgcgga taagctccag atcttgctgg caactggcgc aagtccgaca accctgaacg
     2281 gccaggcgtc ttcgttcatc tatcggatcg ccacactcac aacaatgagt ggcagataca
     2341 gccaggtagt tcaggcggcg catttttatt gctgtgttgc gctgtaattc ttctatttct
     2401 gatgctgaat caatgatgtc tgccatcttt cattaatccc tgaactgttg gttaatacgc
     2461 ttgagggtga atgcgaacaa taaaaaagga gcctgtagct ccctgataat tttgcttttc
     2521 atgttcatcg ttccttaaag acgccgttta acatgccgat tgccaggctt aaatgagtcg
     2581 gtgtgaatcc cttcagcgtt accgtttcgc ggtgcttctt cagtacgcta cggcaaatgt
     2641 cattgacgtt tttatccgga aactgctgtc tgactttttt gatttcagaa ttagcctgac
     2701 gggcaatgct gcgaagggca ttttcttgct gaggtgtcac tgaacaagcc ccatgtcggc
     2761 aagcataagc acacagaata tgaagcccgc tgccagaaaa atgcattcag tggttgtcat
     2821 acctggtctc tctcatctgc ttctgctttc gccaccatca tttccagctt ttgtgaaagg
     2881 gatgtggcta acgtatgaaa ttcttcgtct gtttctgctg gtattggcac aaacctgact
     2941 ccaatttgag caaggctatg tgccaaatca atactcgttc ttaactcaac aggagatgct
     3001 ttgtgcatac cgccccccgt ttattattta tctcctcagc cagccgctgg gctttcagcg
     3061 gatttcggat aacagaaaga ccgtgaaata cccagcctcg ctttgtaacg gagtagacga
     3121 aagtgatcgc gcctacccgg atattatcgt gaggatgggt catcgccatt gctctccaaa
     3181 tacaaaacca atttcagcca gtgcttcgtc cattttttcg atgaactccg gcaccatctc
     3241 gtcaaaactc gccatgtact tttcatcccg ctcaaccacg acataatgca ggccttcacg
     3301 cttcattcgt gggtcatagt tggcaaagta ccaggcatct tttcgtgtca cccacatgct
     3361 gtactgcacc tgggccatgt aagccgactt tatggcctcg aaaccaccga gccggaactt
     3421 catgaaatcc cgggaggtaa acgggcattt cagctcaagg ccattgccgt cactgcataa
     3481 accatcggga gagcaggcgg tgcgcatact ttcgtcgcga tagatgatcg gggattcagt
     3541 aacattcacg ccggaagtga attcaaacag ggttctggcg tcgttctcgt actgttttcc
     3601 ccacgccagc gccttagcat taacttccgg agccacaccg gtgcaaacct cagccagcag
     3661 ggtgtggaag taggacattt tcatgtcagg ccacttcttt cctgagcggg gctttgctat
     3721 cacgttgtga acttctgaag cggtgatgac gccgagccgt aatttgtgcc atgcatcatc
     3781 cccctgttcg acagctctca cgtcgatccc ggtacgctgc aggataatgt ccggtgtcat
     3841 gctgccacct tctgctcagt ggctttctgt ttcaggaatc caagagcttt cactgcttcg
     3901 gcctgtgtca gttctgacga tgcgcgaatg tcgcggcgaa atatctggga acagagcggc
     3961 aataagtcgt catcccatgt tttgtccagg gcaatcagca gagtgttaat ctcctgcatg
     4021 gtttcatcgt taaccggagt gatgtcgcgt tccggctgac gttccgcagt gtatgcagta
     4081 ttttcgacaa tgcgctcggc ttcatccttg tcatagatac cagcaaatcc gaaggccaga
     4141 cgggcacact gaatcatggc tttatgccgt aacatccgtt tgggatgcga ctgccacggc
     4201 cccgtgattt ctctgccttc gcgagttttg aatggttcgc ggcggcattc atccatccat
     4261 tcggtaacgc agatcggatg attgcggtct ttgcggtaaa tccggcacgt acatgattca
     4321 ttgtcctgct caaagtccat gccatcaaac tgctggtttt cattgatgat gcgggaccag
     4381 ccatcaacgc ccaccaccgg aacgatgccg ttctgcttat cagggaaggc gtaaatttct
     4441 ttcgtccacg gattaaggcc gtactggttg gcgacgatca gcaatgcgat gaactgcgca
     4501 tcgctggcat cacctttaaa tgccgtctga cgaagagtgg tgatcagttc ctgtgggtcg
     4561 acagaatcca tgccgacacg ttcagccagc ttcccagcca gcgttgcgag tgctgtactc
     4621 atccgtttta tacctctgaa tcaatatcaa catggtggtg agcaatggtt tcaaccatgt
     4681 accggatgtg ttctgccatg cgctcctgaa actcaacatc gtcatcaaac gcacgggtaa
     4741 tggctttttt gctggccccg cagcgttgca aatgatcgat gcagagtgat tcaaacaggt
     4801 gctgtggaag acctttttcc atgtcgtctg ccagttctgc ctctttctct tcacgggcga
     4861 tctgctggta gtgacgcgcc cagctttgag cctcaagacg atcctgaatg taataagcgt
     4921 tcatggctga actcctgaaa atggctgtga aaatatcgcc tgcgaaatgc cgggctgatt
     4981 aggtaaacag gaaaggggat tagtgattca ggccgttgcc gcgtccgtcg agaaaaactt
     5041 ccacgagcaa atcacgggta taagtgcgct cgatgccgcg atgcagataa agccgtccgc
     5101 gtaaattagc tgatgcagtc caggtaccat ctttgtgttt gaccagcatt cctggcatga
     5161 ccgcaccgcg attaacggtc tgcgttccgt aatgttgatg aaccataaaa actcctgccc
     5221 gtaagctggg ctgctgaaca tatagagact tctgcgcgta ttcaggcggt ggatggccgc
     5281 cggttgtcat aactaagccg cctcgttgaa gcgactaagg tatgaaatgt tgagttaatt
     5341 tcagctggtc acaccgacgt tcacgcgtcc gtttcacccc tcgcactccc cgaagcctgc
     5401 tgaaattcaa actgcggatc taagcggtca tcgcaacggt gaatcaggta gttgccgtat
     5461 cgttgtgttg ttgcgatgaa cttatttaaa actatagttg ttttatcgtc aacaacaaaa
     5521 gttgttttat tggttgtttt agatataact ggttgtattt aggatggatt tattttgtga
     5581 cttgaatcgc atagcgataa ctgaagcgag gttatggtgg tttttttaac ggtgtgtgtg
     5641 atgaggggag ggcaaaagaa aacccggcac ggtgaccggg attcttacgc cgttaggtaa
     5701 agatattatt gcggtggctt aatattacta cctagagcaa agataggaat tagttcttta
     5761 ctgaatgagc acaatgccca gttgataatt tttaattggt actacccatg cttcctatat
     5821 gtctgcggca tgctcccaat aaccttaccg aagatgaaca cccggttcat ctcgtctttc
     5881 tcgatcgggt cccacggtga gtagcttttg ttatcagaga tgaccagcag cttatccttc
     5941 atcatttgca ggcgctttac atgggctgtg tcgtcgtaca gaaacgcata gataccatca
     6001 ccgtcgaaag atttaactgt gatatcaacg aacagcagat cacctggttc gatcgttcct
     6061 gacatgctgt caccacgcac gttaatgatg cggatatttt ccgccttcct accatcgaac
     6121 atgtgacgag catcgtcaaa cgagtactca accgagcgta gaacttctac aaactcacgg
     6181 ttgatgactc ccggcccagc actgacttct atatcaagaa cgtcaatctt gaagtatttg
     6241 gaatggctga cagttgattg tattggttgc actgtactgt ctgacatatt tccaacgcca
     6301 gaagataacc attctgcgcg cacacccaaa gcgttcgcga tctccacgat tttagttgtt
     6361 tgattagctt tccctgtttc gattttctga atagcagcct ggctaacccc gaccaaatcc
     6421 ccaagcgcct tttgtgtaag gcctcgcgct aatctggctt ctttaagtct ttctgagagt
     6481 gttgttttca tagtccaaat gtacaaccaa ggttttattc catcaaacga aaatggttgt
     6541 tgactaaaaa caaccatagt tttaatcttg attcaaatta accacggagg ttgttatgaa
     6601 cccagctatc aaaacagcga tcaatatcgt tggttcacaa aagaaactgg gcgctgcttg
     6661 cgaagtttca cagcaggccg tctataagtg gcttcacaac aaagcaaagg tatcccctga
     6721 acatgtcggc agcattgtta cggctactgg tggagtagtg aaggcatacc agattcgccc
     6781 ggatcttccg aagttgtttc cacacaccga aaagaacgca gcttaaattt ccatttcacg
     6841 ctctttaaca ataagcaatc aacttaacag tcaattcaaa ctaaaggagt caattatgca
     6901 accacttaca taccaacaga ctagcggatt tagcccgact gcggtgataa atcgttctca
     6961 aacaaaacag gtgccaggcc acgaaaaaat ccgtgatgcc gtccgcgcct ggtcggctgt
     7021 agataatcag gatgtcgttg ccacactcat tgtgaatgag tatctggagc agggcggcgg
     7081 caccatcgat ttccctgatg atgtcagccg tgcacgccag aagctgttcc gcttcctcga
     7141 taacaaattc gattctgaaa aataccgaaa taacgtgcgt gaactgaccc cggcaattct
     7201 ggcggtacta ccgctggaat atcgcggtta cctggttgag caggatagct tcatggctag
     7261 gttggctgaa atggaaaagg aactcagtga ggcaaaacag gctgtcattc tcaacgcacc
     7321 acgccaccag aaactgaagg aaattagtga aggtattgtg tcgatgtttc gtgtggaccc
     7381 agatctggct ggtccattga tggcgatggt tactaccatg ctgggggcga tatgacaggt
     7441 tcagaaatgg cgaaagccgg tctgctggaa cagaaccgac tttcaggtgc aaatcgtaac
     7501 acactcattg cgggaggaat tatggcaaac actgctgaga tattcaattt tccagtgccg
     7561 gatgcggcac aaaaggagcc gcgcgtggca gatctcgatg atggttatac gcgcattgca
     7621 aatgagttgc tggaagctgt gatgctggcc ggattaacac agcaccagct tctggtcttt
     7681 ctggctgtca tgcgcaaaac atatggcttt aataaaaaac tggattgggt tagcaacgag
     7741 caactttccg aattaaccgg gatattgccg cacaagtgtt ctgctgcaaa aagtgttctg
     7801 gtaaagcgtg ggatttttat tcagagcggg cggaataccg gcattaataa tgtggtcagt
     7861 gaatggtcaa cattacccga atcaggtaag aaaaataaag tttacctgaa agaggtaaat
     7921 ttacctgaat caggtaaaaa aagtttaccc aaatcaggta aaggcgttta cccgaatcag
     7981 gtaaacacaa aagacaaact aacaaaagac aatataaaac ctttttcgtc cgagaattct
     8041 ggcgaatcct ctgaccaacc agaaaacgat cttcctgtgg tgaaaccgga tgctgcaatt
     8101 cagagcggca gcaagtgggg gacagcagaa gacctgaccg ccgcagagtg gatgtttgac
     8161 atggtgaaga ccatcgcgcc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
     8221 atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg acatgtgcgt gctgttccgc
     8281 tgggcatgcc aggacaactt ctggtccggt aacgtgctaa gtccggccaa actccgcgac
     8341 aagtggaccc aactcgaaat caaccgtaac aagcaacagg caggcgtgac agctagcaaa
     8401 ccaaaactcg acctgacaaa cacagactgg atttacgggg tggatctatg aaaaacatcg
     8461 ccgcacagat gattaacttt gaccgtgagc agatgcgtcg gatcgccaac aacatgccgg
     8521 aacagtacga cgaaaagccg caggtacagc aggtagcgca gataatcaac ggtgtattca
     8581 gccagttact ggcaactttc ccggcgagcc tggctaatcg tgaccagaac gaactgaacg
     8641 aaatccgccg ccagtgggtt ctggctttcc gggaaaacgg gatcaccacg atggagcagg
     8701 tgagcgacgg aatgcgtgtt gcccgtcggc agaatagacc atttctgcca tcacccgggc
     8761 agtttgttgc atggtgccgg gaagaagcat ccgttaccgc cgggctgcca aacgtcagcg
     8821 agctggttga tatggtttac gagtattgcc ggaagcgtgg tctgtatccg gatgcagagt
     8881 cttatccgtg gaaatcaaac gcgcactact ggctggttac caacctgtat cagaacatgc
     8941 gggccaatgc gttgactgac gcggaattac ggcgcaaggc tgccgatgaa ctggcctgta
     9001 tgaccgcgcg aattaaccgt ggtgaggcta tacctgaacc agtaaaacaa cttcctgtca
     9061 tgggcggtag gcctctaaat cgtgcacagg ctctggcgaa gatcgcagaa atcaaagcta
     9121 agttcgggct gaaaggagcg atgaaataga gctaaagtat tagcttaaaa ataaagtata
     9181 cctagcgaat ataatctagg actagttgta acgcccgaaa tgattgattg cgtgagcaac
     9241 aaaatttttc ttccattgtt gtgaaatcat ttagccataa agtttatgca gagattttat
     9301 tttatttttg agttgatttt tttgtaacga gctgtatatt tcgcgcctct tgtcatacca
     9361 gttatttttt tcattatatt cataacgttg attgaactat gaccactgtg aataaaaagt
     9421 taaaaaaaac tgcatctggc gcgattacat ggtcggtaat tgttacccag atattatctc
     9481 cagtttctct ttctttgatc ccagcaaaca gttttgcatc atctggtaat aaagatgtta
     9541 cgcaaattta tgccagcgat gagcatgcaa ataaagtggc ctcatttgca gcaagtgcag
     9601 gtcagagcct ggcgaataat aatgcaagta gttttgctgt aaatacttta tcaactcagg
     9661 cgacaaaaga ggtcgtcgac tggttgcaac aatatggcaa tgcgcgcatc aagcttaatg
     9721 tcgatgattc tttttccttg aaggattcat cattcgactt tttatatcca tggctggata
     9781 ctcaggatta tgtgttattc agtcagacat cactacatcg tactgatgac cgaaatcaga
     9841 ccaatattgg tttggggatt cgtcatttca ctcctgataa cgcaatgttg ggtgcgaatg
     9901 ttttctatga ttacgattta agtcgtagtc actctcgtgc aggttttggg gttgagtact
     9961 ggagggatta tttcaggctt ggtgtaaata cctattttgg tctatctgac tggaagaaca
    10021 gtcgtgatat tgatgattat ctggaaagac ctgcaaatgg ctgggatttt tctgctgaag
    10081 gatggctacc tgcttatccg caattagggg catctattca gtttgaaaaa tattatggta
    10141 aaaatgtcgg gttatttgga agcgataacc tgcaggaaaa cccttacgca gttactgggg
    10201 gaatttctta tacaccagtt cctctgatta agttttctgc acagcataag caaggacaga
    10261 gcaacactca cgatacaacc tttggtgttg agtttaatta tcgcccgggc gtttccttgg
    10321 ctgaacagct tagcagcgat aatgttgcag ttatgcgaga agtccagaac cggcgttatg
    10381 attttgttga acgaaataac aacattgttc tggagtacaa gaagaagcat gcactgaaaa
    10441 tcagtttgcc agagtctgtt cagggggagg gggaatcaat catccctgta acactgacaa
    10501 tcaacaatgc tagtggtggt atcaagtctg tacagtggaa tgatagtgca ttcactgcgg
    10561 ctggcggcaa gatcagtgga aatggcacat catggcagat cactttaccg gcttataaaa
    10621 gcgaaggtgt aaattcgtgg aatgttggag ccacagtaca ggataacaga ggcaacgttt
    10681 ccaactatgc cgtgatgaat attagcgtta ctgatagtgg tgtctcgacg gctgattcgt
    10741 cttttacact ggatggagat tcgtcgccga c
//
LOCUS       DAOFSV010000145        10609 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.145, whole genome shotgun sequence.
ACCESSION   DAOFSV010000145 DAOFSV010000000
VERSION     DAOFSV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.145"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..151)
                     /locus_tag="QBH27_003881"
     CDS             complement(<1..151)
                     /locus_tag="QBH27_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDQ7016139.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            764..1525
                     /locus_tag="QBH27_003882"
     CDS             764..1525
                     /locus_tag="QBH27_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016140.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSVMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            1546..3039
                     /locus_tag="QBH27_003883"
     CDS             1546..3039
                     /locus_tag="QBH27_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDQ7016141.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            3168..4427
                     /locus_tag="QBH27_003884"
     CDS             3168..4427
                     /locus_tag="QBH27_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDQ7016142.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            4662..5828
                     /gene="nhaA"
                     /locus_tag="QBH27_003885"
     CDS             4662..5828
                     /gene="nhaA"
                     /locus_tag="QBH27_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDQ7016143.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5894..6793
                     /gene="nhaR"
                     /locus_tag="QBH27_003886"
     CDS             5894..6793
                     /gene="nhaR"
                     /locus_tag="QBH27_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDQ7016144.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(6851..8890)
                     /locus_tag="QBH27_003887"
     CDS             complement(6851..8890)
                     /locus_tag="QBH27_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDQ7016145.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPMMRNE
                     NWALTEEKGDAVAYITYEEKQATLQNGKITAIVNLQGQISFWRNAEKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKYTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNRGYGFLWNNPAVGRVTFAQNGTEWVAEVSEQLDYWV
                     TAGDTPAEISAAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYDYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMTAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKSYISKIMAETHEKNSPIMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQTLHHCAPLHQIPVFIREKGKYRQIFPV"
     gene            complement(8918..10291)
                     /locus_tag="QBH27_003888"
     CDS             complement(8918..10291)
                     /locus_tag="QBH27_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016146.1"
                     /translation="MSSVLETDKSSASDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKTIYSAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFSVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWILMVLANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLINSLATIQILFIIAIPFFSRYLSKTWIWITGLLVAMFGGGLMWLAGDNIPLMIA
                     AWMLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAWIMNSFGYIPNQPQTAAGLDGISWAFIWVPAVLFALAAIPLLFFRKYEAMEECIQH
                     DLQVHNP"
BASE COUNT         2849 a         2346 c         2503 g         2911 t
ORIGIN      
        1 actcggattc gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct
       61 cacagaggtc ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca
      121 gggcgacaat catcgcctta tgctgcttca ttgctctctt ctccttgacc tttcggtcag
      181 taagaggcac tctacatgtg ttctgcatat agggggcctc gggttgatgg taaaatatca
      241 ctcggggctt ttctctatct gccgttcagc caatgcctga gacagacagc ctcaagcacc
      301 gccgctatta tactgcgctt ttaattcact tctgttttat caattctttt tctgaagacg
      361 cctcgcattt ttgcggaata atatttcaat acatcaattt atttaactta aatatatcca
      421 gggattcaca aaaaaggtgt tttcctctat caaaagtgaa tggctaaagg aaattaatta
      481 ttaaaggtta gtgtaatact atagagagac aaagaaatac gttcagcagc caatgctaag
      541 ttcatcatga aaaatgccta taaatcaaag ggtgatattt aaaacagaca cattttctga
      601 aacacgatat ccctccgccc gttcgcaaaa gtaatctgcg atcttcatca cgcttcgata
      661 gtcatcttta aatatactgg cctggtcatt cttaataaaa atgaattgtc tggtgtatta
      721 attcctatgg aatcataaaa acaatgaatg gagaacatat tatatgtctg ccaaaagacg
      781 acttcttatt gcgtgtaccc tgataacagc tatctatcat tttcctgcat attcttcatt
      841 agaatataaa ggaacctttg gttcaataaa tgcgggttat gcagactgga atagtggatt
      901 tgtaaacact caccgtggtg aagtatggaa agtgactgcg gattttgggg taaattttaa
      961 agaagcagaa ttttactcat tttatgaaag taatgtactc aatcatgctg tagcagggcg
     1021 aaatcatacg gtttcagtaa tgacgcatgt cagactcttt gactctgata tgacattctt
     1081 tggcaaaatt tatggccaat gggataactc atggggtgac gatctggaca tgttttatgg
     1141 attcggttac ctcggctgga acggcgagtg gggctttttt aaaccgtata ttggattaca
     1201 taatcaatct ggtgactacg tatcagctaa atatggtcaa acgaatggtt ggaatggtta
     1261 tgttgttggc tggacagcag tattaccatt tacgttattt gacgaaaaat ttgttttatc
     1321 taactggaat gaaatagaac tggacaggaa cgatgcttac acggagcagc aatttggccg
     1381 gaacgggtta aatggcggtt taactattgc ctggaagttc tatcctcgct ggaaagccag
     1441 tgtgacgtgg cgttatttcg ataataagct gggctacgat ggctttggcg atcaaatgat
     1501 ttatatgctt ggttatgatt tctaattttt tatcagggaa taattatgca gaaaacgtta
     1561 atggccagtt tgatcggcct tgcagtttgc acagggaatg cttttagtcc tgccttagcc
     1621 gcagaggcta aacaacctaa tttagtcatt attatggcgg atgatttagg ttatggcgat
     1681 ttagcaacat atggtcatca gatcgttaaa acacctaata tcgacaggct tgcccaggaa
     1741 ggggtcaaat ttactgacta ctatgccccc gctcctttaa gttcaccttc acgcgcaggg
     1801 ctattaaccg gccggatgcc atttcgtact ggaattcgct catggattcc ttcaggcaaa
     1861 gatgttgcct tagggcgtaa cgaactcacg attgctaatc tactcaaagc gcaagggtac
     1921 gacacggcaa tgatgggtaa gctgcatctg aatgcaggca gcgatcgcac cgatcagcca
     1981 caagcacaag atatgggctt tgattactca ctggttaata cggcgggctt tgttaccgac
     2041 gccacgctgg ataacgctaa agaacgcccg cgttatggca tggtttaccc gacaggctgg
     2101 ctacgtaacg ggcaacccac tccacgagcc gataaaatga gcggtgagta tgtcagttcg
     2161 gaagtcgtca actggctgga taacaaaaag gacagcaagc ctttcttcct ctatgttgct
     2221 tttaccgaag tgcatagccc cctggcttcg cccaaaaaat acctcgatat gtactcacaa
     2281 tatatgagcg cgtatcagaa gcagcatcct gatttatttt atggcgactg ggcagacaaa
     2341 ccctggcgtg gtgtggggga atattatgcc aatatcagct atctggatgc acaggttgga
     2401 aaagtgctgg ataaaatcaa agctgtgggt gaagaagata acacaatcgt tatttttacc
     2461 agtgataacg gtccggtaac gcgtgaagcg cgcaaagtgt atgagctgaa tttggcaggg
     2521 gaaacggatg gattacgcgg tcgcaaggat aacctttggg aaggcggaat tcgtgttcca
     2581 gccattatta aatatggtaa acatctacca cagggaatgg tttcagatac acccgtttat
     2641 ggtctggact ggatgcctac tttagcgaaa atgatgaact tcaaattacc tacagaccgt
     2701 actttcgatg gtgaatcgct ggttcctgtt cttgagcaaa aagcattgaa acgcgaaaag
     2761 ccattaattt tcgggattga tatgccattc caggatgatc caaccgatga atgggcgatc
     2821 cgtgatggtg actggaagat gattatcgat cgcaataata aaccgaaata tctctacaat
     2881 ctgaaatctg atcgttatga aacacttaat ctgatcggta aaaaaccaga tattgaaaaa
     2941 cagatgtatg gtaagttttt aaaatataaa actgatattg ataatgattc tctaatgaaa
     3001 gccagaggtg ataaaccaga agcggtgacc tggggctaac ctgataatca taataaatgt
     3061 aatgtatgcc atttagggct atgcccgcct gattcagtgc tacgcattga atcaggttct
     3121 tttcgcgttg aacaatatca agatacggct attcgcaggg agtggatatg tttaccaacg
     3181 taaatgttga ttgttgcaaa acaccaggat gtaaaaacct ggggttgctg aatagccagg
     3241 attatgtcgc acagggtaaa aatattttat gccgtgaatg tggttacttg tttccagtga
     3301 tatctgaaca gtcgcttaat atttatcgta atattgtgaa tcactcctgg agaggtttga
     3361 tttgccaatg ttcaacttgc ggaggcacgt ccctcaaaaa atatggatat tctgcacaag
     3421 gccagagaag aatgtattgc catcattgtg agaaaacatt tatcactctg gaacatgtaa
     3481 ttaccacacc acgaggagcc cagttagcat tgatgattga gcaaggggag gcacttgcgg
     3541 atatcagaaa gtcattactt cttaacagca ctggacttag ccgtgaactg ttaaaattag
     3601 cgcgtgaagc aaactataaa gaaagtcgac agtgtttccc agcttctgat attaccctga
     3661 gtacccgcgc ttttcgcgtc aagtataatg gtagcaataa ctctctttat gctcttgtta
     3721 ccgcagaaga acaaagcggc agggtggttg ccatctcaac caattactcc ccatctgccg
     3781 tagagcaaca ttatcaatac acatcgaact atgaagagcg tatgtctcca gggacgctgg
     3841 cacatcatgt ccagcgcaaa gagttactta ctatgcggcg ggataccttg tttgatattg
     3901 attacggccc ggcagtttta catcaaaacg atccgggaat gttggtaaaa ccggttcttc
     3961 cggcatatcg tcattttgaa ctggtcagaa tactgaccga tgagcattcc aacaacgttc
     4021 agcattacct tgatcacgaa tgctttatat tgggcggctg cctgatggct aatttgcagc
     4081 atattcatca aggtcgctgc catatttcct ttgtcaaaga gcgcggtgtg gcacccgcca
     4141 ccattgattt tccgccgcga ttattcctta gtggtggggt acgaaataat gtctggcgtg
     4201 cattttctaa ccgcaattat tcaatggctg tatgcaatct cactggcagt aagaaagtcc
     4261 gcgagatgcg gcatgcaaca ttgaacagtg cgacgcgttt tatccacttt gtggagaacc
     4321 atcctttcct tatatcattg aaccgaatgt ctcctgcgaa tgtcgtttct acattagata
     4381 tcctcaaaca tctgtggaat aaaaaactag agcatggaac aatttaatta ttacttttcc
     4441 gaataatttc ttcagaaatc ttgacattag actatgatta atggggttta aggtcatcag
     4501 ccgccgcttt cgggcatcaa aaactcatct ctcgctgatg gcgcaattct tttaatagat
     4561 cgtaaaaagc gagctattcc tacactataa tctgatttta acgatgattc gtgcggggta
     4621 aaatcgtgaa aacgatctat tcacctgaag agaaataaaa agtgaaacat ctgcatcgat
     4681 tctttagcag tgatgcctcg ggaggcatta ttctcattat tgccgctgta ttagcgatga
     4741 ttatggccaa cagcggtgca accagtggat ggtatcacga ctttcttgag acgccggttc
     4801 agctccgggt tgggacactt gagatcaaca agaacatgct gctatggatc aatgacgctc
     4861 tgatggcggt atttttcctg ttggttggtc tggaagttaa acgcgagctg atgcaaggtt
     4921 cgctggccag tctgcgccag gcggcatttc ctgttattgc cgcaatcggc gggatgattg
     4981 tcccggcatt gctctatctg gcttttaact atgccgatcc tatcactcgc gaaggctggg
     5041 caatcccggc ggcgactgac attgcctttg cacttggtgt gttggcgctg ttgggaagtc
     5101 gtgttccgtt agcgctgaag atctttttga tggctctggc tattatcgac gatcttgggg
     5161 ccatcattat catcgcattg ttctacacta atgacttatc gatggcctct cttggcgtcg
     5221 cggctgtagc aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc cgcacgggcg
     5281 tttatattct ggttggcgtg gtgctgtgga cagcggtgtt gaaatcaggt gtccatgcaa
     5341 cgctggctgg cgtcattgtc ggcttcttta ttccgttaaa agagaagcat gggcgctctc
     5401 cggctaaacg tctggagcat gttttgcatc catgggtggc gtatctgatt ttgccgctgt
     5461 ttgcatttgc taatgctggc gtttcactgc aaggtgtcac gctggaaggt ttgacctcca
     5521 ttctgccatt agggatcatc gctggtttgc tgattggcaa gccactgggt attagtctgt
     5581 tctgctggtt ggcgctgcgt ttgaaactgg cacatctgcc agagggaacg acttaccagc
     5641 aaattatggc ggttggtatc ctgtgcggta tcggttttac tatgtctatc tttattgcca
     5701 gcctggcatt tggtagcgta gatccagaac tgattaactg ggcaaaatta ggtatccttg
     5761 tcggttcaat ttcttcggcg gtaattggat atagctggtt acgcgttcgt ttacgtccat
     5821 cagtttgaca ggacgatgta ctggggagcc ataaacggct cccttttcat tgttatcagg
     5881 gagagaaatg agcatgtctc atatcaatta caaccacttg tattacttct ggcatgtcta
     5941 caaagaaggt tctgtggttg gcgcagcgga ggcgctttat ttaacaccac aaaccattac
     6001 cgggcagatc cgggcgctgg aagagcgcct gcaagggaaa ctatttaagc gtaaaggacg
     6061 tggtctggaa cccagcgaac tgggggaact ggtctatcgc tatgccgata aaatgttcac
     6121 cttaagccag gaaatgctgg atatcgtcaa ctatcgcaaa gagtccaact tattgtttga
     6181 tgttggtgtg gcagatgcac tttccaaacg tctggtcagt agcgtactta acgccgcagt
     6241 ggtagaaggc gagccgattc atcttcgctg cttcgaatcc acccacgaaa tgctgctgga
     6301 gcaattaagt cagcataaac tggatatgat catttccgac tgtccgatag attctacgca
     6361 gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc gtgagtttct ggtgtacaaa
     6421 tccaccaccg gaaaaaccgt tcccggcttg tctggaagaa cggcggcttt tgattcctgg
     6481 gcgacgttca atgttagggc gcaaattgct taactggttt aactcccagg gattaaacgt
     6541 agaaatcctt ggcgagtttg atgatgccgc gttgatgaaa gcttttggtg cgatgcacaa
     6601 tgcaatcttc gttgctccaa cgctttatgc atatgacttt tatgccgata aaactgtcgt
     6661 agaaattggt cgcgtcgaga atgtgatgga agagtaccat gctatttttg ctgagcggat
     6721 gattcagcac ccggcggtac agcgaatctg caatacggat tattcttcgt tgttttctcc
     6781 cgcctcacga tagctattaa aactcacgcc ccgctacata cggggcgtgc taatgaacaa
     6841 acgatgtcct ttaaacggga aatatctggc gatatttccc tttctcgcga atgaataccg
     6901 gtatctggtg cagcggtgcg caatgatgaa gcgtttgccc acccgcgtag tgttcatggg
     6961 tataaaaatc gatccatgtc tctccagcag gcaaccagac atcacgtgaa cgtgtacctt
     7021 cgtgcattac aggggcaacg agaaggtctg ggccaaagca gtactgatca taaatattcc
     7081 agctttctgc ctggtcagga aattcaaaga acatcgtgcg cattatcggg ctgttcttct
     7141 cgtgcgtctc agccataatt ttactgatat aagatttcag tttttcacgc aactgcaggc
     7201 aggaagtgag aatttcaccg gtttcttcac catagctcca gacttcattc ggcgcgccag
     7261 tcggacattg tgctatgcca tcacgatagg gttgttcagg caaaacctgc gggtcgcggt
     7321 tgccatgtaa acgcattact gggctgaata ccccccactg gaaccagcga attaacagtt
     7381 catggaattt cggatcgtga atattaccgc cgtggaaacc accaatatcg gttgtccacc
     7441 acgggatccc ggcaattccc atgttcaagc cagcggcaaa ctgattacgc aaagaacgga
     7501 aagaggagtg aatatcgcct gaccacacca gagcgccata tttctgactg ccagcccagg
     7561 cacagcgcag caggttaatc acctgctttt caccagccgc ggtcatacca tcgaagaacg
     7621 ttttggcgta catacgcgga tagatattac cgacttccag cacaggccct gcgtagtaac
     7681 gatagttgtc ataatcgtaa acaccgaact ctggctcggc ttcatccagc cagaacagtt
     7741 tcacgccttt atcgtaataa ttgcgtttgg ctttgttcca gacatattcc cgtgcgccag
     7801 gatgggtagc atcaaagaac gtggtgttgc caaggaaatc catattgata ggcaaaccac
     7861 gctcggtgtg gaccagccag ccgttttctt tcatctcccg ataactctcg gtacggttat
     7921 caaccgtggg ccagaaggag accatcagtt caatgcccat ctcttttagt tcggcaatca
     7981 tggcatcggg atcgggccag tcgcgcagat cgaacatcca gtcaccctga ttcggccaat
     8041 ggaagaaatc gatgacaatc accgaaatag gcaaattacg ctgcttgtag ccacgtgcaa
     8101 cttctagtaa ctcttgctga ttacggtagc gcaacttgca ctgccagaat cccatggcgt
     8161 aatcgggcat cattggcggc gtaccggtaa cacgtgcata ggcggcgctg atttctgcgg
     8221 gagtatcccc tgcggtaacc cagtagtcca actgttcgct aacttctgcc acccattccg
     8281 tgccattttg cgcgaaagtg acgcgaccca ccgctgggtt attccacaga aaaccatagc
     8341 cgcgattaga aagcaggaag ggaaccgatg cctgagaatt acgctgcgcc agctctagca
     8401 tacagccttt taaatccaga tttggctgct ggtactgccc catgccaaac aacttttcgc
     8461 catcgttggc ttcgaaccgc gccttgattg tgtatttccc gccgggaatc ggtttgaact
     8521 cgcgggcctg taaattcagg gcgctgacat actgaccgtg ggccgactca tcttcgccaa
     8581 tttcgccgcg ctgacgccag aactcctgca gcaggcattt ttctgcatta cgccagaaag
     8641 agatctgacc ctgcagatta acaatggcgg tgattttgcc gttttgtagt gttgcctgct
     8701 tttcttcata tgtgatatag gcgacagcat cacctttttc ttctgttaat gcccagtttt
     8761 cattgcgcat catcggcaga tggcgaccgc tacgcacacg taaactgttc tcaccccatg
     8821 cttcaatcca gagataacga tcgtcttgtt gccagaccag acgatgggga tcttgcttaa
     8881 taaacgacat aatttcctcc aggagcttgt gacatgttta agggttatga acttgcaaat
     8941 cgtgttggat gcactcttcc atcgcttcat atttacgaaa gaagagcagt ggaatggcgg
     9001 cgagtgcaaa gagcacggct ggcacccaga taaacgccca gctaataccg tccagaccgg
     9061 cggcagtttg tggctggttg ggaatatagc caaagctgtt cataatccag gcggcgaagg
     9121 cggtgccgat accactgccc atcttgatgc agaaggtact gccgaaggcg attagaatgc
     9181 cggtagcttt aatccctgtt ttccagcgac caaaatcaac cgcgaaacca agcatggcga
     9241 agggcataga gcaggcaatg ccactgccaa tattcgccag catccaggcg gcaatcatca
     9301 aaggaatatt gtctccagcc agccacatca gtcctccgcc gaacatggcg accagcaggc
     9361 cggtgatcca aatccaggtt ttggagaggt aacggctgaa aaacggaatt gcgatgataa
     9421 acaaaatctg aatggtggcg aggctgttga tcaagggaac taaatctttg cgatccaggt
     9481 tgtaggttaa atagtaaaca atggttgtgt tgcgctgctg taaggcaatc cagaagatga
     9541 gatttgccag caccatcagg atccacggcc agttgccttt cattgccgaa aagctctttt
     9601 tcatcggcag acgtggttcc tgctgtattt tgtcggtatc cagttctcga atatttttga
     9661 aggcaaatag cgttagcgca caggagatta cggaaaagac gatagcagta taaataaatc
     9721 ctgccttatc attaccgttt ccaagaaaag ccactaacgg tagcgccgtc gcattcatta
     9781 ataacacgcc aatttgcccg ccggtcatcc ggtaagagtt taataccaac cgttcttttg
     9841 gtgataacgt catcaaaggt aatattgcgc ttagcggcgt gtttaaaccg gtaaaaagaa
     9901 tgctggctaa catgtacgat attgctgaat atatggtttt acctgtcata ctgatatctg
     9961 gcgaccagaa tgagagtgcg ctaaatacag caaaaggtaa tggcaaccat aaaaaccacg
    10021 gccgacattt accgtagcgg gaatgcgttt tatcaataat aatcccccat accggggcat
    10081 cgataccgtc tacaatacgc gccagtagca ggatgatgcc agctacggca aggcttatgc
    10141 ccgccacgtc agtgtagaaa tagagaatgt aggtggagcc aaaacagtag agcagattac
    10201 ctgcaacatc cagcgaacca tagctgatcc gttgtattaa aggcagtgaa tcggatgcgg
    10261 atgatttatc agtttcaagt acggaactca ttatattctc tccagaacaa gaatgcctgg
    10321 ctcagaaaaa cgacttttac tttctaagca aatacatgct aactatcctt gatgcgtatt
    10381 tattaatgat gtaaagcaac gttgtggcaa tgagagtaat taaagtgggg aagagcgaca
    10441 attataattt ttcgttttaa ggttgcgtta attagttttt tattcagttt tcctgctatt
    10501 tgttatacag ttcacgtttt acagccaaat gaaatatttt atctttcaca atgaaaaagc
    10561 atcaattata tttatgatgt attcatggag aaaatatata atcgattaa
//
LOCUS       DAOFSV010000146        10605 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.146, whole genome shotgun sequence.
ACCESSION   DAOFSV010000146 DAOFSV010000000
VERSION     DAOFSV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.146"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="QBH27_003889"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="QBH27_003889"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDQ7016147.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
     gene            complement(815..2659)
                     /gene="btuB"
                     /locus_tag="QBH27_003890"
     CDS             complement(815..2659)
                     /gene="btuB"
                     /locus_tag="QBH27_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDQ7016148.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGSRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2724..2914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3028..4128
                     /gene="trmA"
                     /locus_tag="QBH27_003891"
     CDS             3028..4128
                     /gene="trmA"
                     /locus_tag="QBH27_003891"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDQ7016149.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWCQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4168..4527)
                     /locus_tag="QBH27_003892"
     CDS             complement(4168..4527)
                     /locus_tag="QBH27_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDQ7016150.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQDVAE"
     gene            complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="QBH27_003893"
     CDS             complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="QBH27_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDQ7016151.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5281..6597)
                     /locus_tag="QBH27_003894"
     CDS             complement(5281..6597)
                     /locus_tag="QBH27_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016152.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6640..7806)
                     /locus_tag="QBH27_003895"
     CDS             complement(6640..7806)
                     /locus_tag="QBH27_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDQ7016153.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     TQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            8136..9536
                     /gene="sthA"
                     /locus_tag="QBH27_003896"
     CDS             8136..9536
                     /gene="sthA"
                     /locus_tag="QBH27_003896"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDQ7016154.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(9519..10436)
                     /gene="oxyR"
                     /locus_tag="QBH27_003897"
     CDS             complement(9519..10436)
                     /gene="oxyR"
                     /locus_tag="QBH27_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDQ7016155.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
BASE COUNT         2803 a         2867 c         2519 g         2416 t
ORIGIN      
        1 cattcaacca aatcaatcta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaatg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ctctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacca gccgggttcc ctctggcagc acttgtaata gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aactgtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagcgcatc cagagaaaca tcttcgccat gcagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaacagtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcgg tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcactgcg gtgacaattt ccaccactcg
      661 ctcaacaata aacacttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggca agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc gtaatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atatgcaaca gcaagatccc
      961 acaagctcac cccgcccatt ttcactgttt gatacggata agaggagtaa tccttatcat
     1021 aacgagaacc taagtactga taagtaatcc cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcagta atcgcattgc
     1141 gcgcatcaac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac acccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cagttaagcc ttcaaacgcg ccttcccact gtttgctttt ctcagggtcg agattcggat
     1381 ttccgtagaa accatacagt tgtccaagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caataaagcg ataaccttcg ataaattccc aaccggcgct ggtttgccag gtgccatgac
     1501 gaccaaactg tgagttatcg tcgctacgtg ctgcgccttc aaaggtaaaa tcgccaactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataag
     1621 ctgtgcccgg tgccgtgctc tgcttctgcc agtcaacacc cgcaccaata ttaccgtggc
     1681 caatgatgat gttgtttgcc cactggacgg tgtactgttt catctcatcg agcgtcgcgg
     1741 atgaatcata acgaccataa tgcggatcgt agttgtaatc tttgctatgg ctgtagctgg
     1801 taatcagttg tgatttaatc agttcaccgt tatagcgcag tccggcatcc caactttggc
     1861 tatagagttt acgggtatcg accagtggcg agccgggaga ataatatgcg tcataattgg
     1921 tacgattatc atagccatag ccgcgcacaa agccgctcca gacatcagta aaattatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt atctggctgc gcttgcgttc
     2041 cggtattacc ataggcgaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gtgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cggggtgatc gcgcgtcgtg atgatattca
     2221 cgaccccgcc tattgcatct gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcaata gggaactgac taagatcagc agaaccactc acccccgcca
     2341 aattcaggcg gacgccatca attaacacca acacatgact ggcatttgta ccgcgaataa
     2401 aaatagatga gagctgacct gaaccgccgt tttgggtgat atccacgccc ggaagacggc
     2461 gcagcacatc attgaccgag gtcgactgcc agcggtcgat atcctgacgc gtcacaacag
     2521 tagttggcgc aagcacagtg ctgcgcggtt gttcaaaacg gttagcagta acgacgagag
     2581 tatccgggct ggtatcctgt gcccaagcgg aaaatgccgt gacggaacac gccgtcagca
     2641 gcgaagcttt tttaatcatt gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca
     2701 tcaatattac gcgatgatga gaaccagatg cgacgttggc cggcaggtct tcgggcttgg
     2761 aggggtatct aagatactaa gagatgatga cttcccaccg gatggcagtg tccgcataac
     2821 gcaatcatcg cacctttcct taccgctgcg cgtcagctcc agattcgcac tggattccct
     2881 attaactcac aggaccggca agtggatgct acaggttgta acaagttact gtccagacgt
     2941 agctcacaaa taggaattca tcaagatctg gacatctgat gagcaatccc tacaatcgcc
     3001 gcgtacttta atttttcagg atacatcatg acccccgaac accttccaac agaacagtat
     3061 gaagcgcagt tagccgaaaa agtggtacgt ttgcaaagta tgatggcacc gttttctgac
     3121 ctggttccgg aagtgtttcg ctcgccggtc agtcattatc ggatgcgtgc ggagttccgc
     3181 atctggcacg atggcgatga cctgtaccac atcattttcg atcaacaaac caaaagtcgt
     3241 atccgtgtgg atagcttccc cgccgccagt gaacttatca accagttgat gacggcgatg
     3301 attgcgggtg tgcgtaataa tcccattctg cgccacaagt tgttccagat tgattacctc
     3361 actacgctaa gcaatcaagc ggttgtttcc ttgctgtacc ataaaaagct ggatgatgag
     3421 tggtgtcagc aggcggaagc cctgcgcgat gcactgcgcg cgcagaatct gaatgtgcat
     3481 ctgattggtc gggcaacgaa aaccaaaatc gcgctggatc aggattacat cgacgaacgt
     3541 ctgccgatcg cggggaaaga gatgatctac cgtcaggtag aaaacagctt tacccaaccg
     3601 aacgcggcga tgaatattca gatgctggaa tgggcgctgg acgtaaccaa aggctcaaaa
     3661 ggcgatttac tggagctata ctgcggcaac ggtaactttt cattagcgct ggcacgcaat
     3721 tttgatcggg tattagccac cgaaatcgcc aagccgtcag tcgccgccgc gcaatacaac
     3781 attgccgcta accatattga taacgtacaa attattcgta tggcggcaga agaatttact
     3841 caggcgatga atggtgtgcg cgagtttaac cgcctgcaag gaatcgactt aaagagttat
     3901 cagtgcgaaa ccatttttgt cgacccaccg cgcagcggtc tggacagtga aaccgagaaa
     3961 atggtgcagg cgtatccacg tattctgtat atctcctgca atccggaaac gttatgcaag
     4021 aatctggaaa cattaagcca gacgcacaag gtcgaacgtc tggctctatt tgatcagttc
     4081 ccctacacgc accatatgga gtgcggcgta ttactgaccg cgaagtaaaa cccatgtcgg
     4141 atgcgcctgc atccggcatg atgctgatta ctcagcaacg tcctgcttac ggttacgcat
     4201 cttcgcgcca atccagaaca ccataatgac ggacagtact gccgggaaga agttggaacc
     4261 gatatccgga tattccgcac gaactaccgt gctgtacagc agtacgccga gaataaaaca
     4321 agcagctgcc agacccggca agcctaccgg catagtgcga ttaagataac gttgatgcag
     4381 gcagtaaact gtcaacacca gggaaataat cgggaataca gaaaatggta caatggagct
     4441 aaacagcgct gcgaaagtac cattaatcga taagccagcc actagcgcca gcagcagcgt
     4501 acctctatct tgatttgctt gtttcattac tcgtccttca catttcccgg aataattgcg
     4561 gttttctctt gttcacggcg ataccagtaa taagcccctt tcgaaatcat tcgcagctgc
     4621 agtaccagtc gctcttctaa ttgccgacgt tgctcgacgc cgacatccaa cgcctcggcc
     4681 cccgcactga agacaatcgt caccattgct tcggcttgcg cttcagtaaa cgcacgcggc
     4741 atatggtttt cgagttccag atagtccgca agttccgcaa tgaagtgctg aatttcacgc
     4801 gcaacggcgg cacgaaacgc agcggaggtg ccggagcgtt cccgcaataa taaccggaag
     4861 gcgttaggat tattaccgat gaactccata aatgtggaga ccgaggtgcg gatcacactc
     4921 ccgcctttgg cgatacgctg acgcgcctga cgcatgagtt ggcgtagcat taaaccgctc
     4981 tcatcaacca tggtcagacc cagttcgtct acgtcgcgga aatgccgata aaaagaggtg
     5041 ggagcaatgc ctgcttctcg tgccacttca cgcaaactca ggctggcgaa gctgcgttca
     5101 gcacttaatt ggctaaatgc ggcttccacc agcgaacggc gggttttttc tttttgttgc
     5161 gctcttacgc ccatcacgat gtctgaatcc ttgccaaaag tactgctggc actataccag
     5221 agaatgaaca tactggattg ctccagtgtt gtgaatgaac ggtaacgcaa taaaacatat
     5281 ttactgcaat tctttggccg gttcttttac gtacaacaac gccagcatcg acagcaacgt
     5341 gcctccaccc agataccacg ccggagccgt cagactatcc agcaacacga tggattgcgt
     5401 agcgaaatat tgagcaaaac cgccaaagat tgccacacca atgctataaa ccagcgcaaa
     5461 acccaatgcc cgaatccgtt tcggcaacag ttcagagatc aacaacatca ctggcacacc
     5521 gccaagcgtg gtaaagctga ccatcacgaa aaccaccgtc agcaacatgc cagggcttgg
     5581 gtaattcacc aacagccaga atgacggcca gctacagaga agcaccatca cacgggagat
     5641 taaaatcagc tttttacgcc cgaccgaatc gcacaacatc cccacaagca gcgcacccac
     5701 aaacgtaaca acgcccgcca gtaacatcgc tgcatgagaa taatgttgag gcatcgccag
     5761 atacttcgcc gcccaggtgc cgtaataaaa gagggagata taggttgcga ccgtgctacc
     5821 aatagcgagt aaaacgccat taacgatagt cgccttatgt tgcaggagca gagaaaacgc
     5881 gctttcgctg gtagcagctt ttttattgcg cacaggctca ggtacgtcat tttccaggct
     5941 cagacgcaac cagcaaccga taggtgctaa taacacacca ataaaaaacg gcacacgcca
     6001 gccccattct gccattacgg tttctgaacc tgtggcgaac ggtagccatg cgcttaaacc
     6061 taaagcgaca acaccgccaa aagttgttgc caggccttga gtcgccagtg accaactgct
     6121 gtaaaagcca cgacgatttg ccggagctga ctccaccagt agcgacattg aagccccgac
     6181 ttcaccacct gccgcaacgc cctgaatcaa acgcgccagc accagcgttg cggttcccca
     6241 atagcccgcc gtttcatacg tcggtgcgat gccgatcatc agcgtaccga ggctcatcag
     6301 agcaatagtg aacaccatcg ccggtttacg cccgaagcga tcggcccagg caccaacgat
     6361 aatcccaccg agcggacgca taaaaaagct cacaccaaac gttgcaaatg ccagtaacag
     6421 cgcgacgtta tgatcggcac tgggtgggaa gaaaagcgta ctgatataga ccacaaaaaa
     6481 gttgtaaacg ccaaaatcaa aaaattcgag cgcattgcca aacgccgagg caaagatcac
     6541 ccgcctgccg ctgggctttt tcttcacact cacggtttca tttacgatcg ccgtcattcc
     6601 atgttccttg ccaaaaagtg ttgttgtgtg ttgttttatt tattgcagcc aggcttcaac
     6661 cagtgcgccc cagtagctgc ttgccgggac caggcttgca tcgttaaagt cgtaaccggg
     6721 gttatgcacc atgcaggaac cttccccgac atcgccatta ccgaataaca ggaaaccgcc
     6781 tttgggttgg gcctccagca tgaaagagaa atcttcactg cccatcaggg gtttgatgcc
     6841 aaactcagcc cgatcttcac caaatgtttt gcacgccacc tgccaggcga agcgcgccat
     6901 ctcctcgtca tttactaata ccggtgtacc gttaacatgc gtaacctcag ccgtagcgcc
     6961 aaagctggca gcctgggttt gtgcaagagc aggaatacgg gtcagaagaa gttgtcgggt
     7021 atcgcggcta agagagcgga cactgatttt catctcagca ctgtcaggaa tgacgttagc
     7081 cgcctcaccc gcaacaatgc tgccgacggt aattaccgcc gcttccagcg gatcgacatt
     7141 gcgcgacaca atgctttgta atgcggtggt gatatgtgcg gcaaccagca ctggatcgat
     7201 ggctttgtgc gggatcgcac cgtgtccgcc acaaccacga accgtaatat ggaactgatc
     7261 cattgacgcc atcagcgccc caggctggaa gaaaaacttg ccgaccggca aacccggcat
     7321 gttgtgcatc ccgaaaatga catcgcaggg gaaacgatca aaaagcccct ctttaaccat
     7381 gatctcaccg ccgttaatca tctcttccgc tggctggaag ataaggcgta acgtgccgtt
     7441 aaaacggcgg gtttcggcaa aataacgcgc ggcccccagc agtatggtgg tatgcccgtc
     7501 atgcccgcag gcgtgcatta aaccaggatg tttactggcc cagggcttgc cactattttc
     7561 ataaatcggc aacgcatcca tatccgcccg caggccaata cttttctctc cgtcgcccac
     7621 ttttaacgtg gctaccacgc cggttgtcgc cagcccttgc tcaacctcat agccccactg
     7681 gcgcagcttt tccgcaacca aagcactggt tttaaattct tgtaatccca gctccgggtt
     7741 ttcgtggatc tgatggcgga tctcgcgaag ttcttcctca aacccacgaa tatagtgttc
     7801 gatcatcggc tattccttta ttcacttata aacaggctaa ttaacggatt ttattgatgg
     7861 tataaataca aataacccat caatataaaa tatttataca tttgttttat aagaagattt
     7921 cataaagaca ataacaaaaa agcacgcttc ggcaagataa aactatcagc cataagaaaa
     7981 agcgattaat agtgaataaa taaaataaga aggaggaaga caaagcaaag gccgctcacg
     8041 atatagccag ataaatgacg gggatcaatt ggcttacccg cgataaaatg ttaccattct
     8101 gttgctttta tgtataagaa caggtaagcc ctaccatgcc acattcctac gattacgatg
     8161 ccatagtaat aggttccggc cccggcggcg aaggcgctgc gatgggcctg gttaagcaag
     8221 gtgcgcgcgt cgcagttatc gagcgttatc aaaatgttgg cggcggttgc acccactggg
     8281 gcaccatccc gtcgaaagct ctccgtcacg ccgtcagccg cattatagaa ttcaatcaaa
     8341 acccactata cagcgaccat tcccgactgc tccgctcttc ttttgccgat atccttaacc
     8401 atgccgataa cgtgattaat caacaaacgc gcatgcgtca gggattttac gaacgtaatc
     8461 actgtgaaat attgcagggg aacgctcgct ttgttgatga gcatacgttg gcgctggatt
     8521 gcccggacgg cagcgtcgaa acactaactg ctgaaaaatt tgttattgcc tgcggttctc
     8581 gtccatatca tccaacagat gttgatttca cccatccacg catttatgac agcgactcaa
     8641 ttcttagcat gcaccacgaa ccgcgccatg tacttattta cggtgctgga gtgatcggct
     8701 gtgaatatgc gtcgatcttc cgcggtatgg atgtaaaagt ggatctgatc aacacccgcg
     8761 atcgcctgct ggcatttctc gatcaagaga tgtcagattc tctctcctat cacttctgga
     8821 acagtggcgt agtgattcgt cacaacgaag agtacgagaa gatcgaaggc tgtgatgacg
     8881 gtgtgatcat gcatctgaag tcgggtaaaa aactgaaagc tgactgtctg ctctatgcca
     8941 acggtcgcac cggtaatacc gattcgctgg cgttacagaa cattgggctg gaaactgaca
     9001 gtcgcggaca gctgaaggtc aacagcatgt atcagaccgc acagccgcac gtttacgcgg
     9061 tgggcgacgt gattggttat ccgagcctgg cgtcggcagc ctatgaccag gggcgcattg
     9121 ccgcgcaggc gctggtgaaa ggtgaagcca ccgcacatct gattgaagat atccctaccg
     9181 gcatttacac catcccggaa atcagctctg taggcaaaac cgaacagcag ctgaccgcga
     9241 tgaaagtgcc atatgaagta ggccgcgccc agtttaaaca tctggcacgt gcacaaatcg
     9301 tcggcatgaa cgtgggcacg ctgaaaattt tgttccatcg ggaaacaaaa gagattctgg
     9361 gtattcactg ctttggcgag cgcgctgccg aaattattca tatcggtcag gcgattatgg
     9421 aacagaaagg tggcggcaac actattgagt acttcgtcaa caccaccttt aactacccga
     9481 cgatggcgga agcctatcgg gtagctgcgc taaatggctt aaaccgcctg ttttaacact
     9541 ttatcgaaat ggccatccat tcttgcgcgg atggcctctg ccagctgctc atagcggctg
     9601 cgcagcggtg agccaggacg ataaaccagg ccaatagtgc ggcgtggttc cggcttaatg
     9661 cacggcagat aaacaacccc atcgcgtttg cgttccggcg gcacagccag cgcaggcagt
     9721 aaagtgatcc cgctacctgc cgccaccatg ttgcgcagag tttccaggct ggtcgcgcgg
     9781 aagtgtgtat cttcatccgc cccggcttca aaacagaaac ccattgcctg atcgcgcaaa
     9841 cagtgaccat cttccagcat cagcagtttt tcccctgcca gatcggccat cggtacgcat
     9901 tcgcggttcg cccacgggtg gtcttcatag atagccagca acattggctc atcaaacaac
     9961 ggcacttcaa tgaatgcttc gctctctttc accagcgcga ggatcacgca atcgagtttg
    10021 ccgctgtcca gttgcgccag taactggtgg gtctgtgctt catgcagata catttccagc
    10081 tttggaaagg tctggtgcag catcgggata atatgcggta gcaggtacgg tccaaccgtg
    10141 ggaatcaaac caatatgcag cggtccggac atcgtctcgc cctgctggct tgccatctct
    10201 ttaaggactt tcacctcacg cagcacggta cgcgcctgat ccaccagcag cattcccgcc
    10261 tgggtgaata acactttacg gctggtccgc tccagcaaca tcacgcccag ctcatcttcc
    10321 agcttacgaa tttgcccgct aagcgtcggc tggctaacgt ggcaggaatc tgccgcacgc
    10381 cgaaaatggc ggtgttcagc caatgccacc aggtactcaa gatcacgaat attcattatc
    10441 catcctccat cgccacgata gttcatggcg ataggtagaa tagcaatgaa cgattatccc
    10501 tatcaagcat tctgactgat aattgctcac agaaacggag cggcacctct tttaaccctt
    10561 gaagtcacag cccgtttcga gagtttctca gctcgaataa ctaaa
//
LOCUS       DAOFSV010000147        10522 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.147, whole genome shotgun sequence.
ACCESSION   DAOFSV010000147 DAOFSV010000000
VERSION     DAOFSV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.147"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..132
                     /locus_tag="QBH27_003898"
     CDS             <1..132
                     /locus_tag="QBH27_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDQ7016156.1"
                     /translation="DDMEALAFAWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            230..559
                     /gene="mliC"
                     /locus_tag="QBH27_003899"
     CDS             230..559
                     /gene="mliC"
                     /locus_tag="QBH27_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDQ7016157.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            618..1274
                     /gene="pdxH"
                     /locus_tag="QBH27_003900"
     CDS             618..1274
                     /gene="pdxH"
                     /locus_tag="QBH27_003900"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDQ7016158.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1403..2677
                     /gene="tyrS"
                     /locus_tag="QBH27_003901"
     CDS             1403..2677
                     /gene="tyrS"
                     /locus_tag="QBH27_003901"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDQ7016159.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2739..3599
                     /gene="pdxY"
                     /locus_tag="QBH27_003902"
     CDS             2739..3599
                     /gene="pdxY"
                     /locus_tag="QBH27_003902"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDQ7016160.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(3643..4248)
                     /gene="gstA"
                     /locus_tag="QBH27_003903"
     CDS             complement(3643..4248)
                     /gene="gstA"
                     /locus_tag="QBH27_003903"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDQ7016161.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4354..5856)
                     /gene="dtpA"
                     /locus_tag="QBH27_003904"
     CDS             complement(4354..5856)
                     /gene="dtpA"
                     /locus_tag="QBH27_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDQ7016162.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(6467..7102)
                     /gene="nth"
                     /locus_tag="QBH27_003905"
     CDS             complement(6467..7102)
                     /gene="nth"
                     /locus_tag="QBH27_003905"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDQ7016163.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAYGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(7102..7797)
                     /gene="rsxE"
                     /locus_tag="QBH27_003906"
     CDS             complement(7102..7797)
                     /gene="rsxE"
                     /locus_tag="QBH27_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDQ7016164.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(7801..8421)
                     /gene="rsxG"
                     /locus_tag="QBH27_003907"
     CDS             complement(7801..8421)
                     /gene="rsxG"
                     /locus_tag="QBH27_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDQ7016165.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8425..9483)
                     /gene="rsxD"
                     /locus_tag="QBH27_003908"
     CDS             complement(8425..9483)
                     /gene="rsxD"
                     /locus_tag="QBH27_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDQ7016166.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMMLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLTGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9484..>10522)
                     /locus_tag="QBH27_003909"
     CDS             complement(9484..>10522)
                     /locus_tag="QBH27_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915809.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDQ7016167.1"
                     /translation="ARQARLEREKTARLERHKSAAVQPAAKDKDAIAAALARVKEKQA
                     QATQPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAI
                     ARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKA
                     AVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQQANAEPEE
                     QVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQ
                     ANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAK
                     KAAQQKVVNED"
BASE COUNT         2573 a         2659 c         2711 g         2579 t
ORIGIN      
        1 gatgacatgg aagcattggc gttcgcctgg cttgcctggc gaacgctggc gggattacca
       61 ggaaatctgc cttccgtcac tggcgcaagc caggagacgg tactgggggc tattttcccc
      121 gctaacccgt gatatcagag ttaactgaat ttttcttacc gcgtctgccg ttacacttgg
      181 aacgttaggg agggcgtatt gccctccaga ccaggaaagt cttcgggata tgtctatgaa
      241 aaaactgtta ctgatctgtt tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact
      301 ggttgagcgc atgcaaaccg atacgcttga ataccagtgt gatgaaaaac cgttgacggt
      361 caaactgaat aatccgcgcc aggaagtcag ttttgtttac gataatcaac tactgcatct
      421 caaacagggc atttcagcgt ctggtgcacg ttacaccgac ggaatctatg ttttctggtc
      481 gaaaggcgag gaagcgactg tctataaacg cgaccgcatc gtcttgaata actgtcagtt
      541 acaaaatccg cagcgttgag atttttccag gggcggcgca caatagcgtc acccactgac
      601 aatccgtaaa gaaaaccatg tctgataacg acgaattgca gcaaatcgcg catctgcgcc
      661 gtgaatacac caaaggcggg ttacgccgcc gcgatctacc cgccgatcca ttaacccttt
      721 ttgaacgttg gctctctcag gcttgtgaag ccaaactggc tgaccctacc gcgatggtgg
      781 tcgctaccgt ggatgaacat ggtcagcctt atcagcgcat cgttttactc aaacattacg
      841 acgaaaaagg catggtgttt tacaccaacc tcggcagccg taaagcacat caaatcgaaa
      901 ataatccgcg cgttagcctg ctgttcccgt ggcataccct tgaacgccag gtgatggtga
      961 tcggtaaagc ggaacgactt tcgactctcg aagtgatgaa atattttcat agccgcccgc
     1021 gtgatagcca gattggtgca tgggtttcga agcagtccag tcgcatttct gcccgcggta
     1081 tccttgaaag taaattcctg gagctgaagc agaagtttca acagggcgaa gtgccattgc
     1141 caagtttttg gggtggtttt cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg
     1201 agcatcgcct gcatgaccgc tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc
     1261 gtcttgcacc ctgaaaagat gcaaaaatct tgctttaatc gctggtactc ctgattctgg
     1321 cactttattc tatgtctctt tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt
     1381 cgttatatac atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg
     1441 ggggctggta gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc
     1501 gatcgcgctc tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt
     1561 tccattgtta tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg
     1621 cggcgcgacg ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac
     1681 tgaagaaact gttcaggagt gggtggacaa aatccgcaag caggttgccc cgttcctcga
     1741 tttcgactgt ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat
     1801 gaatgtgctg accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa
     1861 caaagaagcg gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt
     1921 ttcctacaac ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt
     1981 gctgcaaatc ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg
     2041 tcgtctgcat cagaatcagg tgtttggctt gaccgttccg ctgatcacca aagcagacgg
     2101 caccaaattt ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc
     2161 gtacaaattc taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa
     2221 gttcttcacc ttcatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag
     2281 cggtaaagca ccgcgcgccc agtatgtgct tgcagaacag gtgacacgtc tggtgcatgg
     2341 tgaagaaggt ttgcaggcgg caaaacgtat taccgaatgc ctgttcagcg gttccttgag
     2401 tgcgctgagt gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat
     2461 ggaaaagggc gcagacctga tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg
     2521 tcaggcgcgt aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga
     2581 tcctgaatac ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg
     2641 cggcaaaaag aattactgtc tgatttgctg gaaataatgc attaagtgga aagggggagt
     2701 gagaaatcac tccccctggt ttttatacag ggaacatgat gaaaaatatt ctcgctatcc
     2761 agtctcacgt tgtttatggt catgcgggta acagtgcggc agagtttccg atgcgccgcc
     2821 tgggcgcgaa cgtctggccg ctgaacaccg ttcaattttc taatcacacc caatacggca
     2881 aatggaccgg ttgcgtgatg ccgcccagcc atttaaccga aattgtgcaa ggcattgccg
     2941 ccattgataa attacacacc tgtgatgccg tattaagtgg ctatctggga tcggcggagc
     3001 agggtgaaca tatcctcggt atcgttcgtc aggtgaaagc agcgaatcca caggcgaaat
     3061 atttttgcga tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg
     3121 cagagtttca tgtgcggcac ggtttgcctg ccagcgatat cattgcgccg aatctggttg
     3181 agctggaaat actctgtgag catccggtaa ataacgtcga agaagcggtt ctggcagcgc
     3241 gcgaactcat tgcgcaaggg ccacaaattg tgttggttaa acacctggcg cgagctggct
     3301 acagccgtga ccgttttgaa atgctgctag tcaccgccga tgaagcctgg catatcagcc
     3361 gtccgctggt ggattttggt atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt
     3421 tactgctggt gaaactgctt cagggggcaa cgctgcagga ggccctggaa catgtgaccg
     3481 ctgcagtata cgaaatcatg gtgaccacaa aagcaatgca ggaatatgag ctgcaagtgg
     3541 ttgctgctca ggatcgtatt gccaatccag aacattattt cagcgcaaca aagctctgaa
     3601 atatctcatt tcggcccaca acgacctgtg ggccgatggc tattacttta agccttccgc
     3661 tgacagcgcg tcttgtactt ccggacgttc agccatacgt tgcataaatg ccgcaatgtg
     3721 ctctaaccct tccagattca gtttcaccgc gtatgcccag cgcagaaccg taaacagata
     3781 ggcatcagca attgtgaatc gttgcccgca gatccagtgc tcatccttca gggcctcgtt
     3841 cacatattgc agcttcttct ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt
     3901 atcagggcga aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt aattcagcca
     3961 ttcgatggtt ttatagcggg aaatactgtt taccggtgcc agcaactggc ggtcggggac
     4021 gctgtcggca agatactgca taatcgctac gccttccgtc agcaaagtac catcgtccag
     4081 cagcaatgca ggtacctgtc ccttagggtt aacggcaaag taatcgtcac cgttttcgag
     4141 acgttttttc attaaatcca cactgacgag cgtaaaatcc tttccgctct cacgcagggt
     4201 gatatgggaa gcgagagagc aggcgcccgg tttgtagaac aatttcatcg gtaactcctt
     4261 ttgtctgtgg tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa
     4321 agttagcggc ttgtaaatga gtttccctga attttacgct acggctgctt tcgccgcttt
     4381 gtctgcagcg tcatcctgcg tcatgcggtg cagtttcggc gcggtaaaca gcatcagtac
     4441 tgcaataacg gcagtagcga caccaatctg caagaatacg cgaccataga cttccagtga
     4501 catcagcgga tcggtaacgt tatccggcac agccatcata cctgcaacat aaccaccaat
     4561 caggtttgca ccggcagtgg tcaggaacca gctacccata atgaagccca tcagacgctg
     4621 cggaacgagt tgtgcaacca ttgccagacc caggccagag atcatcagtt ccccgatact
     4681 ctgcaggcca tagctcgcga ccagccagct tacagacacg ataccagcgt cagacgcgaa
     4741 tttcgcaccc agcggcagaa tcaggaacgc accagaacac atcaccatgc cgatagcaaa
     4801 cttggttggc atcggcaggg tatcgcccat cttgttatag atagcggcca gaatcgggct
     4861 accaatgatg atccagaacg ggttcagtgc ctgatactgc tcaggttcta cggccaggcc
     4921 cagaatggtg tgttcaacgt tacgaatcgc aaagaagttc agtgacgttg gcatctggct
     4981 ataaagcacg aagaagataa tggcttcgag catcaggatg aaggcaacga tcattttacg
     5041 acgcgcagca cctttcatcg cgaaggcttc tttaccgaag ataaccacaa taccgaaggc
     5101 aacaacgccc agcgccatac gcgcaacttc ctgattgtgc agcagccagg tggcgatagc
     5161 gatcagtgcc acaacaccaa taatggtcag cagcaggtta cggtagttga taggctcgaa
     5221 gtctggtttt gaaccgtact gtttaaccca acgttggcag aaggcgaagt taacgatagt
     5281 gatcagcagg cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag
     5341 ccacggcgta gcaatcatag agaagaaaga gccgatgttg acggacatgt agtacatggt
     5401 gaatgcaccg tccagacgcg ggtcgttttt ctcatagcat gtagaaagca gagaagacgg
     5461 gttagcttta aacaggccgt taccgaccgc aatagccgcc atacccatat aaacgatacc
     5521 ggcgtcgtga ccagaccagg caaccagagc ataaccaatc gccagcacaa tagcgccgag
     5581 cataattacg cgtttagtac ccagtacctt gtcacctaac cagccgccga tagcgaccag
     5641 accataaacc agggcactaa aggaagagaa aagggtgatt gaatccgctt cagacatacc
     5701 cagttgttta accaggtaaa cagccataat tccttgtagg ccgtaataac caaaacgttc
     5761 ccataactca atcgagaaga tgagatagaa cgccttcggt tgtttaaaag cgttcaaact
     5821 gacgctttca gttggttttt ggtttgcagt ggacactttt acctcttttt ttacatccca
     5881 tattaacggg ggtgctttct cgcgttatgt ctaaaaaaca tacgcgcaat tgttatagtg
     5941 ggaggggaaa cggcaggtaa tgttcactat cttgcacttt cagacaagtc ctttgtaata
     6001 atctgttaca tataactgag gcggggaatt caaccattcc gggaaatgtt atccagcgtt
     6061 aaatttttca aaaaacgtaa cggcagattt tttcactacc ctgagagggt gatgatggct
     6121 taacggcgat atgttctact atttagcgtt tttcctagtg agatagtcca gtttctgaaa
     6181 aatagccagt gtaatgtttt gtaggtcaat atttgttgtg tgtgtaaatt taaatgattt
     6241 taacctgatt cttgcaatgt ttttacattg ccacagcatc gtgatcttga tcacgaatga
     6301 atagaaaatt tcgctattaa aaaaacaatt aacctgcatt tttggttatc tgcaaacgga
     6361 ttacctgatg ataacgctaa gcaatggcat cattgacgtt aaaaagatgt tttaaaagag
     6421 gataaagaaa ggttatgaat ggggtaatcg gtgttacccc tgatctttag atgtcaactt
     6481 tctctttgta ttcacaaaga tcttcaataa tacaagagcc acagcgaggc ttgcgggcaa
     6541 tgcaggtata acgcccgtgc aggatcaacc aatggtggca gtcgacttta aactctgccg
     6601 gaaccacttt cagtagcttt tcttctacct gttcgacgtt tttccccggc gcaaattgag
     6661 tacgattgca aacgcggaaa atgtgcgtgt cgacagcaat agtcggccag ccgtatgcag
     6721 tgtttaatac gacgttggct gttttacgac ctacaccagg cagggcttca agcgcagcac
     6781 gatcttccgg aacctcgcca ttatgttgtt ccagtaatat acggcaggtt ttgatgatat
     6841 tttctgcttt gctgttataa aggccaatcg ttttgatata ggttttcacc ccttcaacgc
     6901 ccagttcaag catcgctgca ggcgtattag ccaccgggta gagtttcgcc gtcgccttat
     6961 taacactgac atcggtcgcc tgagcggaaa gcagtacggc aatcagcaat tcaaaaggcg
     7021 aactgaaatt aagctcggtg gtgggatgag gattgttctc acgcaggcga gtgaggatct
     7081 ccaggcgttt tgctttgttc atcagacatt ccctgtttca ccgtttggca atgcacgttc
     7141 ggcagctgct tcagtacggc gctttttcat tttttcatca atcaggtatt ttcctgccag
     7201 catcagtccc aggccaataa atgcacctgg tggcagcatc gccagcagga aaggggagtc
     7261 ggtgtggaaa atctccacgc gtaatacttt tgcccagcta cctaacagcg catctgcacc
     7321 gtcaaacaac gtgccattgc cgataatttc gcgtagtgaa cccagcacga acatggcgca
     7381 ggttgcgccc ataccaattg aaaagccgtc cagtgccgaa agcgccggac cttttttggc
     7441 ggcgaaggct tcagcgcggc ccacaacgat acagttagtg acaatcagcg gaataaaaat
     7501 ccctaatgat tgatacaggc caaaggcgta ggcgttgatc agcatctgta cagcgctgac
     7561 caccgaggcg atgatcatca cgtaaatggg aatgcggatc tcggcgggcg tccagtgacg
     7621 cagcgtcgaa atggtcaggt tggttagcgt cagtaccagc gtagtcgcaa gtcctaaacc
     7681 cagagcgtta gtggcagtgg acgtgaccgc caacagagga caaaggccga gcagctggac
     7741 cagcgcagag ttgtttttcc acaacccctg aacaataacg tctttaattt cgctcacgtt
     7801 ttattctcca caggcaggaa gttgagaaag ttgtgccggt aacgtctgag cgtacaatcc
     7861 ggcgcgtttt accgcattaa ccaccgcgcg gggagtaatc gtcgcgccag tgaactggtc
     7921 gaaatcacca ccatctttct tcaccgccca gttcgcatca tccgcaccac tgattttttt
     7981 acccgcaaaa tgggtgatcc agtcagaaag gcgcagttcg attttatcgc caagccctgg
     8041 cgtttcgtgg tgctcagtca cgcgcgtgcc aagtaccgtg ccgttaaaat cggctcccac
     8101 cagcagctga atcgcaccgg aatagccatc tggcgcggtt gcttccagaa cggcagctac
     8161 cggtttgtca tcctgtttgg cgatgtaaac ccgatgctca cctttaccta actctggcgc
     8221 agttaccaga tagcaactct gtgccagcgc attgttatag cgttcagctg gcagcacctg
     8281 atcaaataac gccttttgtt gcagactggc ctgctcagca attgtcgttt tggtcatctg
     8341 gttgatggcc gcagttaacc ctgttgaacc cgctgcaaag agcgccaacg taatgccgtg
     8401 ttttcggata gttttcagca tggtttaccc tttgcgatgg ccgtagacgc gcggacgcgt
     8461 gtagtaatcg atcagaggaa ccgtgatgtt cgccagcagg acggcaaaag ccacgccgtc
     8521 aggatagccg ccgaaactgc ggatcaacca gactaataag cccgccagcg cgccgaaaat
     8581 aagacgacca cgattggtcg tagaagcggt aaccgggtca gtcaaaataa agaatgcgcc
     8641 gagcatggtc gcaccagaca gcagatgaat ttgcggtgct gccagagtat ctggtgagaa
     8701 caaccaaccc aacgttgcgc ataacgccag cgttactaag aagctgaggg gaatatgcca
     8761 gcgaatcgct ttctgccata gcaaccacac gccgccagtc agccaggcga gatttaccca
     8821 ttgccaacca gcgcccgcca gaataccgct gtagatcgga tattgcataa tctcttcaac
     8881 cgaatgaccg gcacggacag aggttttaaa tgtatccagc ggtgtcgcct gactaatgcc
     8941 atcaatacct aagcgtagcg tgttcatatc accaccactg gtggtatgtc cgctgaaaat
     9001 aacctgtatg gcgtcgataa aaccagggat gttgaccgca atttcatgtg gcggtaacca
     9061 gctggtcatc tgcaccggga aggagatcag taagaccaca taaccaatca ttgccggatt
     9121 aaacgggttt tgccccagac cgccatacaa ctgtttagcg atgatcaccg caaacaccgt
     9181 acccagcacg accatccacc atggcgcgag ggggggaata cttaccgcca gcaataagcc
     9241 tgtcagcaat gctgagttat ctttcaacgt tgcagctacc gactgcttgc gtagtttgag
     9301 gacgagagct tcggccagaa gagcactgac cgacgccaac aggatctgaa cgagagtacc
     9361 ccaaccaaaa aaccacagtt gcgctgcgat tcctggcacg gctgcgagca ataccaacat
     9421 cataatgcgc gatgtctggc gctggttatg ggtataaggg gagctagcta ttctgaatac
     9481 catttagtcc tcgtttacaa ccttttgctg ggcggctttt ttggcctgaa cgcgggcaat
     9541 agccgcagca actgccgctt tgcgcggatc aatctgttct tctggctctg cattagcctg
     9601 ttgctgttcc agcttgcgtg ctttggcacg ggcaatagca gcttcgacgg cggctttgcg
     9661 tggatcaacc tgttcttctg gttccgcatt agcctgttgt tgctgttcca gcttgcgcgc
     9721 tttggcgcgt gcaatagccg cttcgacggc agctttgcgc ggatcgacct gttgttctgg
     9781 ctccgcattg gcctgttgct gttccagctt gcgtgctttg gcgcgggcga tagcggcttc
     9841 gacggcggct ttgcgtggat caacctgttc ttctggttcc gcattagcct gttgttgctg
     9901 ttccagcttg cgtgctttgg cacgggcaat agcagcttcg acggcggctt tgcgtggatc
     9961 aacctgttct tctggttccg cattggcctg ttgctgttcc agcttgcgcg ctttggcgcg
    10021 tgcaatagcc gcttcgacgg cagctttgcg cggatcgacc tgttgttctg gttcggcatt
    10081 agcctgttgc tgttccagct tgcgtgcttt ggcacgggca atagcggctt cgacggcggc
    10141 tttgcgcgga tcaacctgtt gttctggctc cgcattggcc tgttgctgct ccagcttgcg
    10201 tgctttggcg cgggcaatag ccgcttcaac ggcagtttta cgtggatcag caacggttgc
    10261 agcgtcgtta gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc
    10321 ccgtgctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg
    10381 tgtagcctgg gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc
    10441 tttggctgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ttttttcgcg
    10501 ctccagacga gcctggcgcg ct
//
LOCUS       DAOFSV010000148        10520 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.148, whole genome shotgun sequence.
ACCESSION   DAOFSV010000148 DAOFSV010000000
VERSION     DAOFSV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.148"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..574
                     /locus_tag="QBH27_003910"
     CDS             <1..574
                     /locus_tag="QBH27_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ7016168.1"
                     /translation="KAPATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILG
                     IGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIP
                     VALMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELA
                     PVVKRAREKVEHDFNQQVINRELASLLQAL"
     gene            585..1979
                     /gene="wcaM"
                     /locus_tag="QBH27_003911"
     CDS             585..1979
                     /gene="wcaM"
                     /locus_tag="QBH27_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDQ7016169.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEGGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            2154..3047
                     /gene="galF"
                     /locus_tag="QBH27_003912"
     CDS             2154..3047
                     /gene="galF"
                     /locus_tag="QBH27_003912"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDQ7016170.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDNASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQAVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            3420..4505
                     /gene="rfbB"
                     /locus_tag="QBH27_003913"
     CDS             3420..4505
                     /gene="rfbB"
                     /locus_tag="QBH27_003913"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDQ7016171.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYFFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNSLEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            4505..5404
                     /gene="rfbD"
                     /locus_tag="QBH27_003914"
     CDS             4505..5404
                     /gene="rfbD"
                     /locus_tag="QBH27_003914"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDQ7016172.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            5462..6340
                     /gene="rfbA"
                     /locus_tag="QBH27_003915"
     CDS             5462..6340
                     /gene="rfbA"
                     /locus_tag="QBH27_003915"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDQ7016173.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            6345..6887
                     /gene="rfbC"
                     /locus_tag="QBH27_003916"
     CDS             6345..6887
                     /gene="rfbC"
                     /locus_tag="QBH27_003916"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDQ7016174.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQRIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSDLAEVLYKTNQYYAPSHEKNIIWNDLLLNIKWPST
                     ALITLSDKDANGERFELSEF"
     gene            6889..8148
                     /gene="wzx"
                     /locus_tag="QBH27_003917"
     CDS             6889..8148
                     /gene="wzx"
                     /locus_tag="QBH27_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O25 family O-antigen flippase"
                     /protein_id="HDQ7016175.1"
                     /translation="MSLLKHSIWNVAGYFIPTLIAIPAFGLIARKIGVELFGLYTLAM
                     IFIGYASIFDAGLTRAVVREIALLKNRVDDCNTIIVTSIIAVIFLGFIGGGGVFLLKG
                     DIIELLNISPIYYADSIKSLVLLSSLIPVFLVTQILLAELEGREYFGILNIQKSVGNS
                     LIAGLPALFVLINQTLFSAIIGVAIARVICLWLSYIMSRERITIDISFFSITVLKRLF
                     RYGGWVTISNIISPILASMDRFILSHIQGASKISFYTVPNELVTRLGIVPGSLGKAVF
                     PKLSHARNFTASYAEQKKAYILMTVIVMPLVLFVYYYAKFILTLWMGAEYAGISVEIL
                     RIMLIGYIFNCYSQISFANIQAFGKAKYTAYIHMMEFIPYLIMLYIISKEYGVIGVAW
                     LWTIRVIIDFLMLLYMSYRCNNLMKKG"
     gene            8153..9118
                     /locus_tag="QBH27_003918"
     CDS             8153..9118
                     /locus_tag="QBH27_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ7016176.1"
                     /translation="MIYIVVLNWNGAIDTINCVKSLMDLNVSDYKIIIVDNCSMDNSY
                     DTIKENLNSLYIADKSIIEVKYEDRNKYKTLENDKIILIQSPQNNGYASGNNIGIEFA
                     LNQENMKYVWVLNNDTEVDKEALTHLISKCDSDKSIGICGSRLVYFADREMQQGLGGV
                     HNKWLCTTKNYEMGRLVSKKYDDEVISNDIDYIIGASMFFSRECLETVGLMNEEYFLY
                     YEELDICLRAKAKNFKLGICSESLVYHKIGASTDGGKSMMADLCSIKNRLVITERFYP
                     QYYWTVWLSLFVVAFNRARRGEFNKMKRCLNVMFNFKRNKGSKCH"
     gene            9181..10329
                     /locus_tag="QBH27_003919"
     CDS             9181..10329
                     /locus_tag="QBH27_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDQ7016177.1"
                     /translation="MKVAFLSAYDPLSTSSWSGTPYYMLKALSKRNISIEILGPVNSY
                     MIYMLKVYKLILRCFGKEYDYSHSKLLSRYYGRIFGRKLKKIDGLDFIIAPAGSSQIA
                     FLKTTIPIIYLSDTTYDQLKSYYPNLNKKTIINDEDASLIERKAIEKATVVSFPSKWA
                     MDFCRNYYRLDFDKLVEIPWGANLFDDIHFANKNIIQKNSYTCLFLGVDWERKGGKTA
                     LKAIEYVRQLYGIDVRLKICGCTPNQKILPTWVELIDKVDKNNVDEYQKFIDVLSNAD
                     ILLLPTIAECYGMVFCEAAAFGLPVVATDTGGVSSIVINERTGILIKDPLDYKHFGNA
                     IHKIISSVETYQNYSQNARIRYNNILHWDNWAKKIIEIMYEHKNRRIK"
     gene            10301..>10520
                     /locus_tag="QBH27_003920"
     CDS             10301..>10520
                     /locus_tag="QBH27_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016178.1"
                     /translation="MSIRIEESNSTKRIICLFILFLVFPDFLFYTLGVDNFSISTIIS
                     ITLLFVFLRAKNICKDNFLIIVALFILLC"
BASE COUNT         3142 a         1862 c         2441 g         3075 t
ORIGIN      
        1 gaaagcgcct gcaacgccgc tggaaatcat ttccgtcgca cgcttaaccg agaaaaaagg
       61 cctgcatgtg gcgattgaag cctgccgtca gttgaaagag cagggcgtgg catttcgcta
      121 tcgcatcctc ggcattggcc cgtgggaacg acgcctgcgc accctcatcg aacaatatca
      181 actggaagat gtggtggaga tgccgggctt taaaccgagc catgaagtga aagagatgct
      241 cgatgacgcg gatgtcttcc tgttgccatc ggttacgggt gcggatggcg atatggaagg
      301 tattccggtg gcgctaatgg aagcgatggc ggtcggtatt ccggtggttt ctactctgca
      361 tagcggaatt cctgaactgg tggaggctga caaatccggc tggctggtgc ctgagaacga
      421 tgctcgcgca ctggcgcaac gactggcggc gtttagccaa ctggacagcg acgaactggc
      481 tcctgtcgtc aaacgtgctc gcgaaaaagt cgaacacgat tttaaccagc aggtgatcaa
      541 tcgagaactc gccagcttgc tgcaggcttt atagaggaga acgcatgcca tttaaaaaac
      601 tctcccgacg caccttcctg acggcaagtt ctgtgcttgc cttcctccat acgcctttcg
      661 cccgcgcact tcccgctcaa caaagcgtta acattaacga ctacaaccct cacgactgga
      721 tcgcctcatt taaacaagcc ttcagcgaag ggcaaacggt cgtcgtgccc gccggattgg
      781 tttgtgacaa tatcaacacc ggcatcttca ttcctcctgg caaaacgtta cacatccttg
      841 gaagcctgcg cggcaacggc aaagggcgat ttgtcttaca ggacggcagc caggtgacag
      901 gggagggggg cggcagtatg cataacatca ccctggatgt gcgcggctct gactgcacca
      961 tcaaagggct ggcgatgagc ggctttggtc cggtaacgca gatttatatc ggcggcaaaa
     1021 acaaacgggt catgcacaac ctgaccatcg ataacctgac cgttagccac gctaattacg
     1081 ccatcttacg ccagggattt cataaccaga taatcggtgc caacatcacc aactgtaagt
     1141 tcagcgactt acagggcgac gctattgaat ggaacgtggc gattaacgac agtgatattt
     1201 tgatatccga ccatgtcatc gagcgcatca actgtactaa tggcaaaatc aactggggca
     1261 ttggcatagg ccttgcggga agcacttatg ataataatta cccagaagat caggcagtga
     1321 aaaactttgt cgtggcgaat atcacgggat cggattgtcg gcaactgatc catgttgaaa
     1381 atggtaaaca ttttgttatc cgtaatatca aagctcgtaa tatcacgccg gatttcagta
     1441 agaaagcagg tattgataac gccacagttg ctatttatgg ttgtgacaac tttgtgattg
     1501 ataatattga aatgattaat agtgccggga tgttaatcgg ctatggggta attaaaggca
     1561 aatatctctc gataccgcag aatttccgag tgaatgatat tcaattggat aatactcatc
     1621 ttgcttataa attgcgcggc atccaaatct ccgccgggaa tgctgtctcc tttgtggcgc
     1681 tgactaacat tgagatgaag cgtgcgtcgc tggagttaca caacaaaccg caacatcttt
     1741 ttatgcgtaa tatcaatgtg atgcaggaat cctcagttgg accagcattg agcatgaact
     1801 ttgacatgcg caaagacgtt cgaggcgtgt ttatggcgaa aaaagaaaca ctgctgtctc
     1861 ttgcaaatgt tcatgcggtg aatgaaaaag ggcaaagctc cgtcgatatc gacaggatta
     1921 atcaccatat tgttaatgtg gaaaagatta actttagatt gccggaacgg agagagtaga
     1981 tttgcgacca ttcctggaaa aatggagtca tacttaggaa caatgctact gcaatccaca
     2041 acgaagcggc gtaacatcac aagtaattca gtaatcaatt cagggtaatt gatgctggcg
     2101 aaaaaaatcg aacaagctat aattcagcaa ccattttaca ggtggatgaa ataatgacga
     2161 atttaaaagc agttattcct gtagcgggtc tagggatgca tatgttgcct gccactaagg
     2221 cgattcccaa agagatgcta ccgatcgtcg acaagccaat gattcagtac attgttgacg
     2281 agattgtggc tgcagggatc aaagaaatcc tcctggtaac tcatgcgtcc aagaacgcgg
     2341 tcgaaaacca cttcgataca tcttatgaat tagaatctct ccttgaactg cgcgtgaagc
     2401 gtcaactgct ggcggaagta cagtccatct gtccgccggg cgtgaccatt atgaacgtgc
     2461 gtcagggcga acctttaggt ttaggccact ccattttgtg tgcgcgacct gccattggtg
     2521 acaacccatt tgtcgtggta ctgccagacg ttgtgatcga caatgccagc gccgacccgc
     2581 tacgttacaa ccttgctgcc atgattgcac gtttcaacga aacgggccgc agccaggtgc
     2641 tggcaaaacg tatgccgggt gacctctctg aatactccgt cattcagact aaagagccgc
     2701 tggatcgtga aggtaaagtc agccgcattg ttgaatttat cgaaaaaccg gatcagccgc
     2761 agacgctgga ctcagacatc atggccgtag gtcgctatgt gctttctgcc gatatttggc
     2821 cggaactgga acgtactcag cctggtgcat ggggacgtat tcagctgact gatgccattg
     2881 ctgagttggc aaaaaaacaa gcagttgacg caatgctgat gactggggac agttacgact
     2941 gcggaaagaa aatgggttat atgcaagcgt ttgtgaagta tgggctgcgt aacctgaagg
     3001 aaggggcgaa gttccgtaaa ggtattgaga agctgttaag cgagtaacaa attcctgact
     3061 ggaagtgtcg gtcagtaagt aattaaaaac ggcagtgaag attcatagca taagttttgc
     3121 tcattgaatc tcactgccgt tgtctttttt ataagtacaa taaaaacagc atgttagctt
     3181 gcaatcatag ttcaaatttt tccaggattt tccttgtttc tacagctgtt tggtaagaca
     3241 attagcgttt gaatttttcg gatttggcac gagtgggtta cgctcgtcac atcgtggaaa
     3301 tgtacgcagt gctctggtag cggttaagcc aggggcggta gcgtgcatta atgcatctat
     3361 taatcaaatc tggagcagtc tatttcacag catgctctct ggctatatgg aataaaaaag
     3421 tgaagatact tgttactggt ggcgcaggat ttattggttc tgctgttgtt cgtcacataa
     3481 taaataatac gcaagatagt gttgttaatg tcgataaatt aacatacgcc ggaaacctgg
     3541 aatcacttgc agatgtttct gattctgaac gctatttctt tgaacatgcg gatatttgtg
     3601 atgcagctgc aatggcacgg atttttgctc agcatcagcc ggatgcagtg atgcacctgg
     3661 cagctgaaag ccatgttgac cgttcaatta caggccctgc ggcatttatt gaaaccaata
     3721 ttgtgggtac ttatgtcctt ttagaagcgg ctcggaatta ttggtctggt ctggatgatg
     3781 aaaagaaaaa aaacttccgt tttcatcata tttctactga tgaggtgtat ggtgacttac
     3841 cccatccgga tgaagtaaat agcaatgaaa cgttgccgct atttacggaa acgacagcat
     3901 acgcgccaag tagtccatat tctgcttcta aagcttccag cgatcatttg gttcgcgcat
     3961 ggaaacgtac ttatggttta ccgaccattg tgactaattg ctcgaacaac tatggtcctt
     4021 atcatttccc ggaaaagctt attccactgg ttattcttaa ttcactggaa ggtaaggcat
     4081 tacctattta tggcaaagga gatcagatcc gcgactggtt gtatgtagag gatcatgctc
     4141 gagcgttata taccgtcgta accgaaggta aagcgggcga aacttataac attggtggac
     4201 acaacgaaaa gaaaaacatc gacgtagtgt tcactatttg tgatttgttg gatgagatag
     4261 tcccgaaaga gaaatcttac cgcgagcaaa ttacttatgt taccgatcgt ccgggacacg
     4321 atcgccgtta tgcgattgat gctgagaaga ttggtcgcga attgggatgg aaaccacagg
     4381 aaacgtttga gagtgggatt cgtaaaacgg tggaatggta cctgtccaat acaaaatggg
     4441 ttgataatgt gaaaagtggt gcctatcaat cgtggattga acagaactat gagggccgcc
     4501 agtaatgaat atcctccttt ttggcaaaac agggcaggta ggttgggaac tacagcgtgc
     4561 tctggcacct ctgggtaatt tgattgctct tgatgttcac tccactgatt actgtggtga
     4621 ttttagtaat cctgaaggtg tagctgaaac cgtaagaagc attcggcctg atattattgt
     4681 caacgcagcc gctcacaccg cagtagacaa agcagaatca gaaccgaagt ttgcacaatt
     4741 actgaacgcg acgagtgtcg aagcgatcgc gaaagcagcc aatgaagtcg gcgcctgggt
     4801 tattcactac tctactgact acgtatttcc ggggaccggt gaaataccat ggcaggagga
     4861 ggatgcaacc gcaccgctaa atgtttacgg tgaaaccaag ttagcgggag aaaaagcatt
     4921 acaagagcat tgtgcgaagc accttatttt ccggaccagc tgggtctatg caggtaaagg
     4981 aaataacttc gccaaaacaa tgttgcgtct ggcaaaagag cgtgaagaat tagccgttat
     5041 taatgatcag tttggtgcgc caactggcgc agagttactg gctgattgta cggcacatgc
     5101 tattcgtgtg gcactgaata aaccggaagt cgcaggcttg taccatctgg tagctagtgg
     5161 taccacaacg tggcacgatt atgctgcgct ggtttttgaa gaggcgcgca aagcaggcat
     5221 tccccttgca ctcaacaagc tcaacgcagt accaacaaca gcctatccta caccagctcg
     5281 tcgtccacat aactctcgcc ttaatacaga aaaatttcag cagaactttg cgcttgtctt
     5341 gcctgactgg caggttggcg tgaaacgaat gcttaacgaa ttatttacga ctacagcaat
     5401 ttaatagttt ttgcatcttg ttcgtaatgg tggagcaaga tgtattaaaa ggaatgatga
     5461 aatgaaaacg cgtaaaggta ttattttggc gggtggttct ggtactcgtc tttatcctgt
     5521 gacgatggcc gtcagtaaac agctgttacc gatttatgat aaaccgatga tctattaccc
     5581 gctctctaca ctgatgttag cgggtattcg cgatattctg attatcagta caccacagga
     5641 tactcctcgt tttcaacaac tgctgggtga cgggagccag tggggcctga atcttcagta
     5701 caaagtgcaa ccgagtccgg atggtcttgc gcaggcgttt attatcggtg aagagtttat
     5761 tggtggtgat gattgtgctt tggtacttgg tgataatatc ttctacggcc acgacctgcc
     5821 gaagttaatg gacgtagctg ttaacaaaga aagtggtgca acggtatttg cctatcacgt
     5881 taatgatcct gaacgttatg gtgtcgtgga gtttgataat aacggtactg caattagcct
     5941 ggaagaaaaa ccgctggaac caaaaagtaa ctatgcggtt actgggcttt atttctatga
     6001 caatgacgtt gtggaaatgg cgaaaaacct taagccttct gcccgaggtg aactggaaat
     6061 taccgatatt aaccgtattt atatggaaca aggacgtttg tctgtcgcta tgatggggcg
     6121 tggctatgca tggctggata cagggacgca tcaaagtctt attgaagcaa gcaacttcat
     6181 tgccaccatt gaagagcgcc agggactaaa ggtttcctgt ccggaagaaa ttgcttatcg
     6241 taaagggttt attgatgctg agcaggtaaa agtattagcc gaaccgttga agaaaaatgc
     6301 ttatggtcag tatctgctca aaatgattaa aggttattaa taagatgaac gtaattaaaa
     6361 ctgaaattcc tgatgtgctg atttttgaac caaaagtttt tggggatgaa cgtggcttct
     6421 tttttgagag ttttaatcag aggatttttg aagaagcagt aggtcgtaag gttgagtttg
     6481 ttcaggataa ccattctaag tccagtaaag gtgttttacg tggtcttcat tatcagttag
     6541 aaccttatgc tcaaggaaaa ctggtgcgct gtgttgttgg cgaggttttt gatgttgcgg
     6601 ttgatattcg taaatcgtca cctacatttg ggaaatgggt tggggtgaat ttgtctgctg
     6661 agaataagcg tcagttgtgg attcctgagg gatttgcaca tggttttttg gtgctgagtg
     6721 atttagcaga agttttatat aaaacgaatc aatattatgc tccatcacat gaaaaaaata
     6781 ttatatggaa tgacctcttg cttaatatta aatggccgag cacagcactg atcactctgt
     6841 ctgataagga tgcaaatggg gaaagatttg aactaagtga gttttgaaat gtctctctta
     6901 aaacatagta tatggaatgt tgcgggctac tttataccaa cattaattgc aattcccgcc
     6961 tttggattaa ttgcgaggaa aattggtgta gaactatttg gtttgtatac gttagcaatg
     7021 atttttatag ggtatgcaag tatatttgat gctgggttaa caagagctgt tgtgcgtgaa
     7081 atagcattac taaaaaacag agtggacgat tgtaatacga taatagtaac ttctattatc
     7141 gctgtgatat ttttagggtt tatcggaggc gggggagtgt ttctgcttaa aggcgatatt
     7201 attgaactgt taaatatctc accaatatat tacgccgatt cgataaagtc tctagtatta
     7261 ttatcatctc tgatacctgt attcttagtc acgcaaatac tattagcaga gcttgagggt
     7321 cgggaatatt ttgggattct aaatatacaa aaaagtgtag ggaattcttt aattgcaggg
     7381 ttacctgcat tatttgtttt aattaatcaa acgctttttt ctgcaattat tggtgtagcg
     7441 attgcaagag ttatatgctt gtggttaagc tacattatga gcagggaaag aataactatc
     7501 gatatctcat ttttttcaat aactgtttta aagcggttat ttagatatgg cgggtgggta
     7561 actataagta acataatatc tcctatatta gcgagtatgg atagatttat tctatcccat
     7621 atccagggag catcaaaaat atcattctat acagtcccta atgagctggt aactaggctt
     7681 ggaatagttc caggctctct tgggaaagct gtttttccaa aattaagtca tgcaaggaat
     7741 tttacagcgt catatgcaga gcaaaaaaaa gcttatatat taatgactgt cattgtaatg
     7801 cctttggttt tatttgtata ttattacgca aagtttattt taacattgtg gatgggggct
     7861 gagtatgcag ggatttcggt cgaaatatta cggattatgc ttatagggta tatttttaac
     7921 tgttattcac aaatctcttt tgccaacata caggcctttg gaaaagcaaa atacactgca
     7981 tacatccata tgatggaatt tattccttat ttgataatgt tatatataat ttcaaaggaa
     8041 tatggggtta ttggtgttgc gtggttatgg acaattcgag taataattga ttttttgatg
     8101 cttttatata tgagttatcg ttgtaataat cttatgaaaa aagggtagcc tgatgatata
     8161 tattgtggta ttaaattgga atggggctat agataccatt aattgtgtta aaagtttaat
     8221 ggatttaaat gttagcgatt ataaaattat cattgttgat aactgttcta tggataactc
     8281 atatgatact ataaaagaaa atcttaattc attatatatt gctgataaaa gtatcattga
     8341 ggtgaagtat gaggatagaa ataaatataa aaccttagaa aacgataaaa tcatattaat
     8401 acaatctccg caaaataatg ggtacgcaag tggtaataat attggcatag agttcgctct
     8461 taatcaggag aatatgaaat acgtctgggt tctgaataat gatactgaag tggataaaga
     8521 ggctttaact catttaatta gtaaatgtga ttcagataaa agtataggga tttgcggttc
     8581 tcgtttagtc tattttgccg acagagagat gcagcaagga ctaggtgggg tgcataacaa
     8641 atggttatgc actacaaaaa attatgaaat gggaagatta gtttccaaaa aatatgatga
     8701 tgaagtcatt agtaatgata tagattatat aattggcgca tcgatgtttt tctctagaga
     8761 atgtttggaa acagttggat tgatgaatga agaatatttt ttatactatg aagagttaga
     8821 tatttgcctc agagcaaaag caaagaactt taaattaggt atttgctcag aaagtttggt
     8881 ttatcataaa ataggtgcaa gtactgatgg gggaaagagc atgatggctg atctttgctc
     8941 aataaaaaat aggctggtca ttacagaaag gttttatccc caatattatt ggacggtatg
     9001 gttgtcactt tttgttgtag catttaaccg tgctagaaga ggtgagttta ataagatgaa
     9061 aagatgtttg aatgttatgt ttaacttcaa acgaaacaaa ggtagcaaat gccattagaa
     9121 tatgcactta atcatggtgt taataaatct atagtttgat atgttattaa agggtattta
     9181 atgaaagtgg cttttttatc tgcttatgat ccactatcta catccagttg gtctggcaca
     9241 ccttattata tgctaaaggc attatcgaag agaaatattt ccattgaaat attaggaccg
     9301 gtaaatagct atatgatata catgttaaaa gtatataaat taatattaag gtgtttcgga
     9361 aaagaatatg attatagtca ttcgaagttg ctttccaggt attacggtag aatattcggt
     9421 aggaaattaa aaaaaattga tggtttggat tttattatcg cacctgcagg ttcctcacaa
     9481 attgcttttt taaaaacaac cataccaata atatatctat cggatacaac atatgatcaa
     9541 ttaaaaagct attatccgaa tttaaataaa aaaacaatta taaatgatga ggatgcaagt
     9601 ttaatcgaac gcaaggctat tgaaaaagca acagtagtat ctttcccatc taaatgggca
     9661 atggattttt gcaggaatta ttacagatta gattttgata aattagttga aataccatgg
     9721 ggggctaatt tatttgatga tattcacttt gctaataaaa atataattca aaagaatagt
     9781 tatacttgtc ttttcttggg agttgattgg gaaagaaaag gtgggaaaac agccttgaaa
     9841 gcaattgaat atgtaaggca gttatatggg atcgatgtta gactaaaaat ttgtggatgt
     9901 actccgaatc aaaagatttt acctacttgg gttgaattaa ttgataaagt agataaaaat
     9961 aacgttgacg aatatcagaa attcatcgat gtgttatcta acgctgatat acttctttta
    10021 ccaaccattg ctgaatgtta tggaatggta ttttgtgaag ctgctgcttt tggattgcct
    10081 gttgtcgcta cagatacagg tggagtcagt tctatagtta tcaacgaaag gacggggata
    10141 ttaattaaag acccgttaga ctataagcac tttggaaatg caattcataa aataattagt
    10201 tccgtagaga cttatcaaaa ctactcccaa aacgcaagaa ttagatataa taatatattg
    10261 cattgggaca attgggctaa aaagataatt gagattatgt atgagcataa gaatagaaga
    10321 atcaaatagc acaaaaagaa ttatatgttt atttatactt tttcttgttt tccctgattt
    10381 tttgttttat acattagggg ttgataattt tagcatttca acgataatct caattacatt
    10441 gctttttgtt tttttaagag ctaaaaatat ttgcaaagat aattttctaa taatagtagc
    10501 gttattcata ttgttgtgtt
//
LOCUS       DAOFSV010000149        10482 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.149, whole genome shotgun sequence.
ACCESSION   DAOFSV010000149 DAOFSV010000000
VERSION     DAOFSV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.149"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..75
                     /locus_tag="QBH27_003921"
     CDS             <1..75
                     /locus_tag="QBH27_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDQ7016179.1"
                     /translation="IAYEGSGSGDWRTDGFIVGVGYKF"
     gene            140..2206
                     /locus_tag="QBH27_003922"
     CDS             140..2206
                     /locus_tag="QBH27_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ7016180.1"
                     /translation="MAAVKISGVLKDGTGKPVQNCTIALKARRTSSTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPE
                     ALRRFELMVNEVARHAGASSQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAAASQ
                     TASANSATAAKKSETNAKNSETATKASEKNAKSSQTAAKTSETNAKDSEANAKVSETA
                     AANSAKASAASQTAAKASEDAAREYANQTAEPYRYVLQPLPDVWIPFNDSLDMITGYS
                     PGYKKVKIGDNVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQ
                     RTNFFQNSTDPSKWNKSTSLDVTETGTDSFGFNYGRFVVQDSIVGTSKAHTIIGLYSS
                     TGGVDTSGDEKHVTISCRVKSEVDNIAVRILFEHYDGEVRTSIGAANLNLTTRIISKT
                     GQTSRVTARSVKDDATGWIFFEATLKADTTENTVGGFVQYSPDTGQMVASGDYLDVTT
                     PQIEAGTGTSSFIVTGTTPATRASDMVTVPIKNNLYNLPFTVLCEVHKNWYKTPNAAP
                     RVFDTGGHQTGAGIVMGFGSSGGYDGFPYCDIGGSNRRINENAGLEKMLIGMRVKSER
                     STCVVSNGKLSSETKTKWEYIRSTATIRIGGQTTAGLRHLFGHVRNFRLWHKELTDAQ
                     LGEVVE"
     gene            2203..2481
                     /locus_tag="QBH27_003923"
     CDS             2203..2481
                     /locus_tag="QBH27_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016181.1"
                     /translation="MRDFTLRFSDKADFRAYLRKLNWEEDEELQNAILVDEIGFTFRE
                     TDVSDDGEPEYTRNEGYFVNIRLLDDGFDDSVFREWVVTPERPLREWF"
     gene            2491..2778
                     /locus_tag="QBH27_003924"
     CDS             2491..2778
                     /locus_tag="QBH27_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016182.1"
                     /translation="MDITSILHALCAVAVQVLAGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRINMPWWGGFDPRAWDVASLMDFAVPVVACLLIWMLIR"
     gene            complement(2896..3144)
                     /locus_tag="QBH27_003925"
     CDS             complement(2896..3144)
                     /locus_tag="QBH27_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDQ7016183.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3499..4065)
                     /locus_tag="QBH27_003926"
     CDS             complement(3499..4065)
                     /locus_tag="QBH27_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ7016184.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            4375..6147
                     /gene="aspS"
                     /locus_tag="QBH27_003927"
     CDS             4375..6147
                     /gene="aspS"
                     /locus_tag="QBH27_003927"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDQ7016185.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGSLRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            6140..6592
                     /gene="nudB"
                     /locus_tag="QBH27_003928"
     CDS             6140..6592
                     /gene="nudB"
                     /locus_tag="QBH27_003928"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDQ7016186.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            6621..7361
                     /locus_tag="QBH27_003929"
     CDS             6621..7361
                     /locus_tag="QBH27_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ7016187.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            7396..7917
                     /gene="ruvC"
                     /locus_tag="QBH27_003930"
     CDS             7396..7917
                     /gene="ruvC"
                     /locus_tag="QBH27_003930"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDQ7016188.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(7919..8521)
                     /locus_tag="QBH27_003931"
     CDS             complement(7919..8521)
                     /locus_tag="QBH27_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDQ7016189.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWNNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDTYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            8592..8657
                     /gene="yobI"
                     /locus_tag="QBH27_003932"
     CDS             8592..8657
                     /gene="yobI"
                     /locus_tag="QBH27_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDQ7016190.1"
                     /translation="MYIFITHFFAEYVIFKYLLPI"
     gene            8796..9407
                     /gene="ruvA"
                     /locus_tag="QBH27_003933"
     CDS             8796..9407
                     /gene="ruvA"
                     /locus_tag="QBH27_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDQ7016191.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            9416..10426
                     /gene="ruvB"
                     /locus_tag="QBH27_003934"
     CDS             9416..10426
                     /gene="ruvB"
                     /locus_tag="QBH27_003934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDQ7016192.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MS"
BASE COUNT         2694 a         2411 c         2832 g         2545 t
ORIGIN      
        1 attgcttatg aaggctccgg cagtggcgac tggcgcactg acggtttcat cgtgggtgtc
       61 ggttataagt tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
      121 gcttttttat ggagtggata tggcagcagt aaaaatctca ggtgtgctga aagatggcac
      181 aggaaaacca gtacagaact gcaccattgc gctgaaggcc agacggacca gcagcacggt
      241 ggtggtgaac acggtggcct ctgaaaatcc ggatgaagcc gggcgttaca gcatggatgt
      301 tgagtatggc cagtacagcg tcaccctgct ggttgaaggt tttccgcctt cacatgccgg
      361 gaccattacc gtctatgaag gttccagacc aggtacgctg aatgattttc tcggcgccat
      421 gacggaggat gatgttcgtc cggaggcact gcgccgtttt gagctgatgg tgaatgaagt
      481 ggcacgtcat gccggagcat catcacagag cgcagcggca gcaaagaaat ccgaaactgc
      541 agcggcatca tcgaagaacg cggcgaaaac ctcagaaacg aatgcagcta acagcgcaca
      601 ggcggcagcg gcctcgcaga ctgcatcggc aaactccgcg acagcagcca aaaaatcaga
      661 aaccaacgcg aaaaatagcg agacagccac aaaggccagc gaaaaaaacg caaaatccag
      721 ccagacggca gcgaaaacca gtgagacgaa tgccaaagac agtgaagcca acgcaaaggt
      781 gagcgaaaca gcggcggcga actcggcgaa agcatcggca gcaagccaga cggcagcaaa
      841 agcaagtgaa gatgctgcca gagaatacgc aaaccagaca gcagagccgt acagatatgt
      901 tttacagccg ctgccggatg tgtggatacc ctttaatgat tcgctggata tgattacggg
      961 ctattctccg ggttataaaa aagtgaagat tggcgataat gtggttcagg ttgccagtga
     1021 taaacaggtt aatttcagtc gcgcatcaac ggcaacatat atcaacaaat ctggcgaact
     1081 gaaaacggcg gaaattaatg agccgcgatt tgagtgtgat ggcctgctta ttgagggaca
     1141 aagaacgaac ttcttccaga acagtacaga cccttcgaag tggaataagt caacttcact
     1201 ggacgttaca gaaacaggca cagatagttt cgggtttaat tatggccggt ttgtcgtaca
     1261 ggattcgatt gttggtacaa gtaaagcgca taccattatc ggactgtatt cgagtaccgg
     1321 aggggttgat acttcagggg acgaaaagca tgtaactata tcctgtcggg taaaaagtga
     1381 agttgataat atcgccgttc gtattttatt tgaacattat gatggggagg taaggacatc
     1441 aataggagca gcaaacctga accttaccac ccgcataatt agcaagacag gtcagacaag
     1501 ccgtgttaca gcaaggtctg ttaaggatga tgcaactggc tggatatttt ttgaggctac
     1561 attaaaagca gatacaacag aaaatacggt tggtggtttt gtccagtatt ctccggatac
     1621 agggcagatg gttgcatcag gggattatct cgatgtaacc actccacaga ttgaggctgg
     1681 tacaggcaca tcatctttta ttgttacggg gacgacaccg gcaacgcggg caagcgatat
     1741 ggtgacagtc ccaatcaaga ataaccttta taatcttcct tttacggttc tttgtgaggt
     1801 acataagaac tggtataaaa cgccaaatgc agcgccgcgt gtttttgata ccggcggtca
     1861 tcaaaccgga gcggggatcg taatggggtt tggttcatca ggtgggtacg acggttttcc
     1921 gtattgcgat ataggtggtt caaaccgacg aataaatgaa aatgccgggc tggaaaaaat
     1981 gcttattggt atgcgggtaa agtccgaacg gtccacatgt gtagtcagta acggtaagtt
     2041 aagcagcgaa actaaaacca aatgggaata tatccggagt acagcaacca ttcgcattgg
     2101 tggacaaact acagcaggat tacgccattt atttgggcat gtgaggaatt ttcgtctctg
     2161 gcataaagag ctaacagatg cgcagcttgg ggaggttgtg gagtgagaga tttcacgttg
     2221 cgtttcagtg ataaagcaga tttcagggca tatctcagga aacttaactg ggaagaggac
     2281 gaagagctgc agaatgccat tctggttgat gagattggtt ttacgttcag ggagacagat
     2341 gtttctgatg acggagaacc agaatacacg cgaaacgaag ggtactttgt taatatccgt
     2401 cttcttgacg atggatttga tgattccgtg ttccgtgagt gggtggttac accagagcgc
     2461 ccgctcaggg agtggtttta aggatagcag atggatatca cgtcgatact tcatgcgctt
     2521 tgtgccgtgg cggtgcaggt actggctggt ctttttaccg gaaactgggc ttacggggcg
     2581 atagccggtt gtacgttctt cattgcgcgt gaacacaccc aggcagaata tcgctggatt
     2641 gaaatgttcg ggcatggcaa gcggattaac atgccgtggt ggggcggttt tgatccacgt
     2701 gcatgggatg tggcaagcct gatggatttt gctgtgccgg tggtggcgtg tctgctgatc
     2761 tggatgttga tccgttaaat ataaaaagta aaaagccgca gtaacttgtc atgacaggat
     2821 actgcggctg gctggttaac tttcgatagt gcgagtattg aatgatttcc agctgttact
     2881 gattttacgt gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag
     2941 agtttcctga acaaaagttt tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc
     3001 atcgttgctg gtggctttta cgatcacctc tacatcgtca taacgtttac tgatgcgtcg
     3061 ggttaattct tcctttaacg catccacagc accggttggc attttagtca ttttttcttt
     3121 ggctatgcag atttcaatac gcataaaagt ccctctatac tgtgtttgta tacagtgtta
     3181 tttttaactg tatagataaa cagtgtcaag ggtgagggaa agtgattttt tggggcatgc
     3241 acggggcaaa aatatttgtt gtggggcatt ttggggcatg attgggacac tttgttacat
     3301 atgaactttg ccgattttca tatgacatgg caatccaacg ctttgattat tctaaagaaa
     3361 acacatgctc ttgggcgttc tttagtgatt tttaaaaatc ccgtatcacg cagttaaagt
     3421 ggcgggcata ctcttcaagg ctggtgatcc ccaaccgcac ccatttagga tgcgaccatt
     3481 gtggcaaacc gatgtaaatc ataacgcgtg gaggatctct tcaacgctac gcacacgggc
     3541 gatgcgcggg tagatatgat taatgctgtt attgtgctgc tcggcgctag cggcgctaca
     3601 ggcatcttcg gcaatcacca gattaaaacc gagttcccag gcattgcggg cggtggattc
     3661 aacaccgata ttggtcgaga tcccacataa cactattgta tcgatacccc ggcggcgcaa
     3721 ctgcaattcc agatccgtac cgtaaaacgc accccattga cgtttgatga tttcgatatc
     3781 gctgtcggtt gcacctaatg ctacaggatg ttgccaccaa ttttcgggca acactttagc
     3841 gggggaaggg gcatcaaccg gctgttttaa tgcttcagca taatcggcag accagccaac
     3901 acgcaccaga aacacgggct gaccgctcgc gcgaaatttc gccgccagct tcccggcgcg
     3961 attaaccacc tcatcggcag tatgtggacc accggcaaaa ggcaagatgc cttcttgtaa
     4021 atcaatcacc accagcgcgg tggttttagc attaagttca agcatgatga ctcctgataa
     4081 aacaaaatcg tgcctctcac cttaatacga tgagccgatg gcggaacgac aaattttgtt
     4141 aatttttatg agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc
     4201 ccgatataaa gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa
     4261 tttccagtat aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact
     4321 tggtcacctg cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt
     4381 acagaatatt gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt
     4441 tgggtcaacc gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa
     4501 ggtatcgtgc aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa
     4561 ctgcgtaatg agttctgcat tcaggtcacg ggcaccgtac gtgcgcgtga cgaaaaaaat
     4621 attaaccgcg atatggcgac tggcgaaatc gaagtactgg cgtcctcgct gactatcatc
     4681 aaccgtgcag atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg
     4741 aaataccgct acctcgacct gcgtcgcccg gaaatggctc agcgcctgaa aacccgcgct
     4801 aaaatcacca gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact
     4861 ccgatgctga ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg
     4921 cacaaaggta aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg
     4981 atgtccggct ttgaccgcta ctatcagatc gttaaatgct tccgtgacga agacctgcgt
     5041 gctgaccgtc agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg
     5101 caagtgcgtg aagtgatgga agcgctggtg cgtcatctgt ggctggaagt gaagggcgtg
     5161 gatctgggcg atttcccggt aatgaccttt gcagaagcag aacgccgtta tggttctgat
     5221 aaaccagatc tacgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt
     5281 gagtttgcag tatttgcagg tccggcgaac gatccgaaag gccgtgttgc cgctctgcgt
     5341 gttccgggcg gcgcatcgtt gacccgtaag cagatcgacg aatacggtaa cttcgttaaa
     5401 atctacggcg cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa
     5461 ggtatcaaca gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggag
     5521 cgtactggcg cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt
     5581 gccgacgcga tgggctcact gcgcctgaaa gtgggtaaag accttggtct gaccgacgaa
     5641 agcaaatggg caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc
     5701 ggcctgacag caatgcacca tccgttcacc tcaccgaaag acatgacggc agcagaactg
     5761 aaagctgcac cggaaaatgc ggtggctaac gcttacgata tggtcatcaa tggttacgaa
     5821 gtgggcggtg gttctgtgcg tatccataat ggtgatatgc agcagacggt gtttggtatt
     5881 ctgggtatca acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa
     5941 tacggtactc cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg
     6001 accggcaccg acaatatccg tgacgttatc gccttcccga aaaccacggc ggcggcgtgt
     6061 ctgatgactg aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag
     6121 gttgtgaaga aggctgagaa taactgatat ggcttataag cgtcccgttt cgatcttagt
     6181 ggtcatctac gcacaagata cgaaacgggt gctgatgttg cagcggcgtg acgatcccga
     6241 tttctggcag tcggtaaccg gcagcgtgga agagggtgaa accgcgccgc aagctgccat
     6301 gcgcgaagta aaggaagagg tcaccattga tgttgtcgct gaacaactga ccttaattga
     6361 ctgtcagcgc acggtagagt ttgaaatttt ttcacattta cgtcatcgct atgcgccggg
     6421 cgtgacgcgt aatacggaat catggttctg tcttgcgctc ccgcacgagc gccagatcgt
     6481 tttcactgaa catctggctt acaagtggct tgatgcgcct gctgcggcgg cgctcactaa
     6541 gtcctggagc aaccggcagg cgattgaaca gtttgtaatt aacgctgcct gaacaggcag
     6601 ggctatctgg agatattttt atggcaggtc atagtaaatg ggccaacacc agacatcgta
     6661 aagctgcgca ggatgctaag cgcggtaaaa tcttcactaa aatcattcgt gagctggtaa
     6721 ccgcggctaa gctgggcggt ggtgatccgg acgctaatcc gcgtctgcgt gcagcaattg
     6781 ataaagcact gtctaacaac atgacccgtg acactctgaa ccgcgcaatt gcacgaggtg
     6841 tgggcggtga tgatgatgca aacatggaaa ccatcatcta cgaaggttac ggtcctggcg
     6901 gcacggcaat catgattgaa tgtctgtctg acaaccgcaa ccgtaccgtt gctgaagtgc
     6961 gtcatgcatt cagcaaatgt ggcggtaacc tcggtactga tggttccgta gcttatctgt
     7021 tcagcaaaaa aggcgtgatc tccttcgaga aaggcgatga agacaccatc atggaagcag
     7081 ccctggaagc cggtgctgaa gacgttgtga cctatgatga cggcgcgatt gatgtctaca
     7141 ctgcatggga agaaatgggc aaagtacgcg acgctctgga agcggcaggt ctgaaagcag
     7201 acagcgcgga agtttccatg atcccgtcca ccaaagctga tatggatgca gaaaccgcac
     7261 cgaaactgat gcgtctgatc gatatgctgg aagattgcga cgacgtgcag gaagtttacc
     7321 ataacggtga aatctctgat gaggttgcag cgactctctg atgaggcctg ctaaacagca
     7381 aaacggagac gcgtgatggc tattattctc ggtattgacc cgggttcgcg cgtgaccggc
     7441 tatggcgtca tccgccaggt aggcaggcaa ctgtcctacc tgggtagcgg atgcatacgc
     7501 accaaagtgg atgatttacc gtctcgtctg aagctcatct atgcgggcgt gacggaaatc
     7561 atcacccaat tccagcctga ttatttcgcc attgaacaag tctttatggc gaagaacgct
     7621 gactcagccc tgaaactggg gcaggcgcgc ggcgtggcga ttgtggcggc ggtgaatcag
     7681 gaattgccgg tatttgaata tgcggcacgt caggtaaagc aaacagtggt aggtattggt
     7741 agtgccgaaa aaagccaggt gcagcatatg gtccgcacct tgctgaaact gcccgctaat
     7801 ccacaggcgg atgccgccga tgcgctggcg attgccatca cccactgcca cgttagtcag
     7861 aatgcgatgc aaatgagtga atcgcgactg aacctggcga gagggcgact gcgttaagtt
     7921 ataccgcctc ggtcagttca ggctgaggcg tttcaactcc caccgcgtga atcaacacca
     7981 aacccggatg atgccacaaa ctggctggcg tgacggtttt acgctcccac ggaatagaac
     8041 ctaagaacca gaatttccag aaagtgagtt ttgcattcgg attgctattt aataattctc
     8101 caaacgtctc tatttcaccc accggaatac atagcgcctc tttataagta tcaaaaacaa
     8161 attttgaaca gaactgtcgc gaagattcgt atttaaaacc ggtgtggtaa agtttgcgta
     8221 gccgggaagg aacctgttca acaattcgtt gtttttgttg ttctgttagt ccggcgtcta
     8281 atcgctttat agcatagcgt tgattagcag agcgtttaat aaaacgggat agcgtagtga
     8341 tggttgagag gggaacacgg ctttctgcaa ccagaaagtc ttcaccgtta tgaccgataa
     8401 tgatcccgac gtgattattc cagcaatttg atgcagctga tatttgacca aataaggcag
     8461 cacctataca tgtaaagacg atatcaccaa tttcatattc cgcaggataa ttaatattca
     8521 cgttgtcatt ccattgaaat agatatgaat atacgtaata gcataaaaaa tttatttttt
     8581 caggagtcgc aatgtatatt tttattacac atttcttcgc tgaatatgta atatttaaat
     8641 atttgcttcc aatataacct gtagaataaa ttatactgcg ccatttttca gatcatcgag
     8701 acacctcgca agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac
     8761 agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca
     8821 tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc
     8881 atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca
     8941 cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc
     9001 gcacgttgtt caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga
     9061 tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgagcgtgaa gaagtggggg
     9121 cactggtgaa actgccgggt attggcaaaa aaaccgccga acgtttgatt gtcgaaatga
     9181 aagaccgatt taaaggtttg catggcgatc tctttacgcc agccgccgac ctggtactca
     9241 cgtctcctgc cagcccggcg accgacgatg ctgaacagga agcggttgct gcgctggtgg
     9301 cactgggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg
     9361 ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat
     9421 tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgtgg cagatcgcgc
     9481 cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt cgcagatgga
     9541 gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatctat tgatttttgg
     9601 tcctccgggg ttgggtaaaa ctacgcttgc caatattgtc gccaatgaaa tgggcgttaa
     9661 tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatctggcag caatgctcac
     9721 taaccttgaa ccacacgatg tactgtttat tgatgagatc caccgtcttt caccagtggt
     9781 ggaagaggtg ttgtatccgg caatggaaga ttaccaactg gatatcatga ttggtgaagg
     9841 tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg gtgcaaccac
     9901 gcgcgcaggt tcgctgacat caccgttgcg cgatcgtttt ggtattgtgc aacgtctgga
     9961 gttttatcag gtgccggatc tgcaatatat cgtcagtcgc agcgcacgct ttatggggct
    10021 tgagatgagt gatgacggcg cgctggaagt tgctcgtcgt gcgcgcggta cgccacgtat
    10081 tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat
    10141 ctcggcagat atcgctgctc aggcgctgga tatgttgaat gtcgatgctg aaggtttcga
    10201 ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggcg ggccggtagg
    10261 tctggataac ctggcggcag ccattggcga agaacgtgaa accattgagg atgtactgga
    10321 accttatttg attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac
    10381 gcgggcgtgg aatcactttg gcataacgcc gccagaaatg tcgtaagtcg gattggtcgc
    10441 tcgtgccgca tccgacactg gcagtgtgcc tgatgcgacg ct
//
LOCUS       DAOFSV010000150        10471 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.150, whole genome shotgun sequence.
ACCESSION   DAOFSV010000150 DAOFSV010000000
VERSION     DAOFSV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.150"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..2235
                     /locus_tag="QBH27_003935"
     CDS             <1..2235
                     /locus_tag="QBH27_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDQ7016193.1"
                     /translation="VEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQ
                     TVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGT
                     TLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSI
                     RENDDGTYAITAVQHVPEKETIVDNGAHFDGEQSGTVNGVTPPAVQHLTAEVTADSGE
                     YQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLALGNYRLTV
                     RAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSE
                     KRIADIRQVEASARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPS
                     DDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKL
                     EDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVL
                     LAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPTFSLTPDGR
                     LSARNADISGNVNANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRNGSYA
                     SGTITVTVYDDQAFDRQIVVPPVLFRGGKHENFNSNNQQSYWYSTCKLQVLKNGQEIF
                     QQPATDVSRVFSSVIDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLLVVVMKKSTAG
                     ISIS"
     gene            2294..4315
                     /locus_tag="QBH27_003936"
     CDS             2294..4315
                     /locus_tag="QBH27_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ7016194.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVALLVEGFPPSHAGTITVYEDSKPGTLNDFLGAATEDDVCPEA
                     LYRFEKMVEEAARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPCANIVPPLPDVWIPFNDSLDMITGFSP
                     SYKKIVIGDDEITMPGDKIVKFKRASTATYINKSGQLKLAEVDEPRFERDGLLIEGQR
                     TNYLRNSNKPDSWTVHSALNKTFGTDKQGFNYATVTPTESIVGTTGGYTVHGVVAADR
                     FPLASGECFTFSCRVKGAKARCRLRVSVIIGGTDTFSADSYLDLDTRIATVSGNTSLI
                     TAKAEQQGEWTYYEATYTANTDIDTVNCAFYMTNKISNETFYDDSTLTMTTPQIELGN
                     TASSFIVTTMPTTRASDVVTIPSQNNLSTRPFTVLCEVSRNWSTPPNVAPRIFDVGGH
                     SIDDNYLSLGFVATGKISANVGMVQPQIASDGERFIVGVRAKSDLSVNAICNGNYTTN
                     LNGKIFGVTATSYRFGGQTAAGTRHLFGHIRNFRVWFKELNDRQIKEAV"
     gene            4312..4590
                     /locus_tag="QBH27_003937"
     CDS             4312..4590
                     /locus_tag="QBH27_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016195.1"
                     /translation="MKDLTLKFPGNREFKSFLSSLDWEEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCVRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            4603..4896
                     /locus_tag="QBH27_003938"
     CDS             4603..4896
                     /locus_tag="QBH27_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016196.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            complement(4988..5845)
                     /gene="sitD"
                     /locus_tag="QBH27_003939"
     CDS             complement(4988..5845)
                     /gene="sitD"
                     /locus_tag="QBH27_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDQ7016197.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGIWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(5842..6699)
                     /gene="sitC"
                     /locus_tag="QBH27_003940"
     CDS             complement(5842..6699)
                     /gene="sitC"
                     /locus_tag="QBH27_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDQ7016198.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(6696..7523)
                     /gene="sitB"
                     /locus_tag="QBH27_003941"
     CDS             complement(6696..7523)
                     /gene="sitB"
                     /locus_tag="QBH27_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDQ7016199.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEDVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(7523..8437)
                     /gene="sitA"
                     /locus_tag="QBH27_003942"
     CDS             complement(7523..8437)
                     /gene="sitA"
                     /locus_tag="QBH27_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDQ7016200.1"
                     /translation="MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            9136..9894
                     /locus_tag="QBH27_003943"
     CDS             9136..9894
                     /locus_tag="QBH27_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016201.1"
                     /translation="MKTLSGKEFRKLFNRYETGFYLSEFEYLKDFDPLFPECVGKELK
                     LGYKRKVDVWCEEDAALYAEFIATKERLKRGRLILKINQVWSRVYPAYIYAIEERINV
                     RAKGRESFKYETVQEKQCKLSIMLDGLKDLECEIAMISSEFEIEKHSMHYFYMIFDIE
                     RYINDCSYFVFSAAKVVKGVIQCIGYIDKNNKASCDEFYSGSYECLDDDQETLNYLNE
                     SCAEGIDADKDLIRKNELLALSLKELYQLLVSKK"
     gene            10366..>10471
                     /locus_tag="QBH27_003944"
     CDS             10366..>10471
                     /locus_tag="QBH27_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HDQ7016202.1"
                     /translation="MGWTEAADLIVKGMEGAINAKTVTYDFERLMEGAK"
BASE COUNT         2916 a         2332 c         2711 g         2512 t
ORIGIN      
        1 gtggaggata cgcaggccat tgcccgttac ggccgtaacg tcacgaagat ggatgccttt
       61 ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa aacggagctg
      121 ctggaaacgc agaccgtgga cttcagcgtg ggtgctgaag ggcttcgcca tgtaccgggg
      181 gatgtcattg aaatctgcga tgatgactat gccggtatca gcaccggtgg tcgtgtgctg
      241 gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct gccatcctcc
      301 ggtaccacgc tgataagcct ggttgacgga agtggcaatc cggtcagcgt ggaggttcag
      361 tccgtcaccg acggcgtgaa ggtgaaagtg agccgtgttc ctgacggtgt tgctgaatac
      421 agcgtatggg ggctgaagct gccgacgctg cgccagcgac tgttccgctg cgtgagtatc
      481 cgtgaaaacg acgacggcac gtatgccatc accgccgtgc agcatgtgcc ggaaaaagag
      541 accatcgtgg ataacggggc gcactttgac ggcgaacaga gtggcacggt gaatggtgtc
      601 acgccgcccg cggtgcagca cctgaccgca gaagtcaccg cagacagcgg ggaatatcag
      661 gtgctggcgc gatgggacac accgaaggtg gtgaaggggg tgagctttat gcttcgcctg
      721 accgtggcag cggatgacgg cagtgagcgg ctggtcagca cggccaggac gacggaaacc
      781 acataccgct tcaggcaact ggcgctgggg aactacaggc tgacagtccg ggcggtaaat
      841 gcgtgggggc agcaggggga tccggcgtcg gtatcgttcc ggattgccgc accggcagcg
      901 ccatcgcgga ttgagctgac gccgggctat tttcagataa ccgcaacgcc gcatcttgcc
      961 gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaaagcggat tgcggatatc
     1021 aggcaggttg aagccagcgc gcgttatctt ggtacggcgc tgtactggat agccgccagt
     1081 atcaatatca aaccgggcca tgattattat ttttacattc gcagtgtgaa caccgttggc
     1141 aaatcggcat ttgtggaggc tgttggccag ccgagtgatg atgcatccgg ctatctggat
     1201 tttttcaaag gagagatagg gaaaacccat ctggctcagg agttgtggac gcagattgat
     1261 aacggtcagc ttgcgcctga cctggctgaa atcaggacgt ccattacgga tgtcagcaat
     1321 gaaatcacgc agaccgtcaa taagaaactg gaagaccaga gtgcagcgat ccagcagata
     1381 cagaaggttc aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcag
     1441 cagatgcagg acggacgcct ttatatcgcg ggtattggtg ccggtattga gaatacccct
     1501 gacggtatgc agagtcaggt gctgctggcg gcggacagga ttgcgatgat taatcctgcg
     1561 aatggcaata caaagccgat gtttgttggg cagggcgatc agatattcat gaacgaagtg
     1621 ttcctgaaat atctgacggc tcccaccatt accagcggtg gcaatccccc aacgttttca
     1681 ctgacacctg atggtcgact ttctgcgaga aatgcggata tcagcggtaa cgtgaatgcg
     1741 aactccggga cgctcaataa tgtcacgatt aaccagaact gtcggattct gggaaaactg
     1801 tcagctaacc agattgaagg tgatattgtc aaaacggtgg gaaaagcctt tccgagaaat
     1861 ggcagttatg ccagcggtac aataacggtc actgtgtacg atgaccaggc ttttgaccgt
     1921 cagatagtcg tcccccccgt tctgtttcgc ggtggtaagc atgaaaactt caacagcaac
     1981 aaccaacagt catactggta ttcaacctgt aagctgcagg tgctgaagaa cggacaggaa
     2041 atctttcagc aacccgcgac ggatgtcagc agggtatttt catcagtcat tgatatgcct
     2101 gccggacacg gccatgtcac cctgactttc aatgtttctt catatggtgc aaataactgg
     2161 acgccaacga ccagtatcag cgaccttctt gttgtcgtga tgaagaaatc aacagccggc
     2221 atcagtatca gttgaatttt aaaacccaaa ttcgggcacc agaaatggtg ccttttttat
     2281 ttgtggagtg aatatggcag tacagatttc aggtgtgctg aaagacggtg caggaaaacc
     2341 gatacagaac tgcaccattc agctcaaagc aaaacgtaac agcaccacag ttgtggtgaa
     2401 cacggtggcc tcagaaaatc cggatgaagc cggacgttac agcatggatg ttgagtatgg
     2461 ccagtacagc gtcgccctgc tggttgaagg ttttccgcct tcacatgccg ggaccatcac
     2521 cgtgtatgag gattctaagc cggggacatt gaatgatttt ctgggcgctg caacagaaga
     2581 tgatgtttgt ccggaggcac tgtatcgttt tgaaaagatg gtggaagagg cggcacgcaa
     2641 cgctgaagcc gcctctcaga gcgcagcggc agcaaagaaa tcagaaacag cagcggcatc
     2701 gtccaggaac gcggcgaaaa catcagagac gaatgcaggt aacagcgcga aagcggcagc
     2761 ttcttcaaaa acagccgcac aaaacgcagc aacagcggca gaacgttcag agacaaatgc
     2821 ccgtgcgtca gaagaagcct ccgcagacag tgaagaggct tcccgccgta atgcagagtc
     2881 agccgctgaa aatgccggag tcgccaccac aaaagcgcgg gaggccgcag cagacgcaac
     2941 aaaggccggg cagaaaaagg atgaggctct gtcggcagcg acacgagctg aaaaggcggc
     3001 agaccgcgca gaagccgcag cggaagtgac tgcagagccc tgtgcgaata tagtgccgcc
     3061 gctgcctgat gtgtggatac cgtttaacga ttcactggat atgattacgg gtttttcgcc
     3121 atcttataaa aagatagtta ttggtgacga tgaaataaca atgccaggcg acaagattgt
     3181 taagtttaaa cgtgcttcaa cagcaacgta tattaataag tccggccaac tcaagcttgc
     3241 tgaagttgac gaaccgcgat ttgagcgcga tggcttattg attgaaggac agaggacaaa
     3301 ttatctgagg aactcaaata aaccagactc atggactgtt cattccgcac tgaataaaac
     3361 atttggcact gataaacagg ggttcaatta tgccacggtg acacccacgg aaagtatagt
     3421 gggaacaaca ggtggctata ctgtgcatgg tgtggttgca gcagacagat tcccgctggc
     3481 aagtggtgaa tgtttcactt tttcgtgccg ggttaaaggc gctaaagcac gatgcaggtt
     3541 aagagtttca gttattattg gtggaacaga taccttctct gctgactctt atcttgatct
     3601 ggatacccgg atcgcaacag taagcggtaa tacatccctt ataacagcca aagctgaaca
     3661 acagggcgag tggacctact atgaggccac ttatacagct aatacggaca ttgataccgt
     3721 taactgtgct ttttatatga caaataaaat aagtaatgag acattctatg atgactcaac
     3781 attaaccatg acgacgccgc aaattgaact gggcaatacg gcatcgtcat ttattgtaac
     3841 tacaatgcca acaacacgcg caagtgatgt agttactatc ccatcgcaga ataacctgtc
     3901 aacacggcct tttacggtat tgtgcgaagt aagcaggaac tggagtacac cgcccaatgt
     3961 tgcgccaagg atatttgatg ttggagggca cagtattgat gataattatt tatcgctggg
     4021 gtttgttgca acaggaaaga taagcgccaa cgtaggaatg gttcagccac aaattgcctc
     4081 agatggagaa aggttcattg tgggtgtgag agctaaatct gatttatcag taaatgcaat
     4141 atgcaatggt aattatacaa caaaccttaa tggtaaaata tttggagtta cagcaacatc
     4201 gtaccggttt ggtgggcaga ccgcagcagg aacgcgtcat ttgtttggac acatcagaaa
     4261 tttcagagtc tggtttaaag aattaaatga caggcaaatc aaggaggcag tatgaaagat
     4321 ttaactttga aatttcctgg taacagagag tttaaatcct tcctgtcatc tcttgactgg
     4381 gaggaggatg aagacctcca gaataaactg ttagtcgatg aaattggttt cacctacaca
     4441 gaaacagggg taacagaaga gggagaacct gtctgtgtcc ggaatgacgg ttattttgtc
     4501 aacattcgca ttcttgatga cttgtttgat gtttctgtat tctctgatta tgtcgtggag
     4561 ctggaaacac cgcttcggga atggagctga aaggaggaaa taatggatat aagcccctta
     4621 cttcatgcac tttgtgctgt ggctgcgcag atactggttg gtctttttac cggaaactgg
     4681 gcttacggag cgatagccgg ttgtacgttc ttcattgcgc gtgaacatac ccaggcagaa
     4741 tatcgctgga ttgaaatgtt cgggcatggc aagcgaatga atatgccgtg gtggggcggt
     4801 tttgatccgc gagcttggga tgtggcaagc ctgatggatt ttgctgtgcc ggtggtggcg
     4861 tgtctgctgg tctggctgtt ggttaatcgt gggtgaaaaa aggtgagctg tatatgcaac
     4921 ggaggaagaa accccgttgc tggaagccag gaaaaagtat cgggtgttgc tgtcctcgga
     4981 tgttgtgcta attgggtgat aacagatcct gtgtatggga gtttcccttt ttgcgtgcgt
     5041 tgatgctggt aacggcaaac gtcatgatga acaaaaccgc gaataagacg acaatcgtcg
     5101 gagctggtgc gctgtcgata aaaaaggaca accagatacc agtcattgac actattaccg
     5161 atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt accgcaatgg
     5221 cgccaggcgc aatgagcaga gaaatggaga gaatgatacc taccgctttc agcgtcgcca
     5281 caatggtcag agagaccatg cacagcagcc cataatgcag ccatctcgta tgcaaaccgc
     5341 ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat tttacgctaa
     5401 taacaagtgt aaccagcccg gcaataatca tcgtctggat tatatcgccg atggttatcc
     5461 cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttgact gcaatataca
     5521 agatcagacc tgcggcaaac atgccagaaa aaacaatccc catcactgta tcttgcttga
     5581 ttcggctgtt gtctttcagg tatcccgtcg ccaccgcaca gaatactccg gcgacgaatg
     5641 cccctgtagc caaaggtaac cccagaatcc aggcaagcac gatgccagga aaaaccgcat
     5701 gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc gacagcagcg
     5761 cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag gtgaactgca
     5821 gtggctccag taataatgcc atcatgagcg atcctccagc gccttatgcg cacgccgacg
     5881 atttgccaaa agcccatggg tgggcgcaaa gacaaatgct aaaagaaaca gcagtgtctg
     5941 cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc acgcgccgag
     6001 gaaactggtt acactgccaa tagttacagc aatcatgagc aggcggggaa aacgatcggt
     6061 taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa aggcaccaac
     6121 cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga gttttaatct
     6181 gcccggatgc aatccaatag cacgggcgtg attctcgtcg aaaaaggtca ccataagatc
     6241 tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga gttgcaggat
     6301 atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt tcacagatgt
     6361 cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga tcagaccgat
     6421 gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc ccgcagccag
     6481 tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg cccccgcaac
     6541 acccgggaca atagagtgcg acaacgcatc gccaatcagc gaccagcctt ttagcatcag
     6601 atagcatgac aggaaagcgc agagaccgcc gaccatcgcc gagacccaca tcgcattgag
     6661 catatattca tagctgaagg gttccagtag cacgttcatc tttcttccct ctgcaccgct
     6721 gacggtcgat gagccacaaa agggcgttca tcgtcagtaa taatgctttc ttccgagccg
     6781 ttaagtgtga catggcgcag tacgccgcta aaagccagct ctaggttctc ggctgtaaaa
     6841 gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt ataatcgcaa
     6901 aatgtcgtga cggatccgag attgtgtgtt gagacaagca tcgttttgcc ttccgcgcgt
     6961 aactccctca acaggctgat gattttggct tcggttttca catcaacgcc agtaaaaggt
     7021 tcatccagca ggatcacatc accctgctgt gcaatcgctc tcgccagaaa gacgcgtttc
     7081 ttttgtccac cggaaagctc gccgatttgt cgatggcgaa aattcaccat atcgacacgt
     7141 tccagcgcat cggtgacaat ttgacgatcc ctctttttgg cgatacgcaa catacccata
     7201 tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga ccagtcaaca
     7261 tcttctgact gagggacgta ggcaaccagg tttttctgta acgcctgtcg cgtgggaata
     7321 cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc tttaaacaac
     7381 gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact tggtactgtg
     7441 aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac atcattaacg
     7501 acaatgcctg cagattgcat cattatttct cccttttccc agctttaatt ccctgtacca
     7561 gcgtactggt agtaacctta agaaggtcga tatacgtcgg taccgggccg ttttctgtgc
     7621 tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc gcaacctgac
     7681 gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga tttttcttaa
     7741 ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca ttaatcggcc
     7801 acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc ccttcactgg
     7861 tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg ggggcaaggg
     7921 tttgggtaat cttggcttta taagtatcgg cattgcgttg gtaggtttgt gcatttgccg
     7981 gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca ttatctggcg
     8041 acatccatgc atgggggtta ggtttgccct catagggtcc ttcggtgatc cctacaggcg
     8101 tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa aagcgttgga
     8161 accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc gcacgtttaa
     8221 tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt atggatgaga
     8281 cttctgcagc atctcccgcc acgtttttgg ccatatccgc gatgatggta aatgttgtaa
     8341 tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg agtgcgagcc
     8401 cccccaagag catggttact ttttttatcg agtgcataat ttagtaccta tagtgagcaa
     8461 aattagaaca aatatagcaa cggctatgtt ttatagcaca gcctatacaa aatgaaaatg
     8521 attatcatta gcagctttga ggcccaaaac agggattagg ttgtttcctg ctggcgacgc
     8581 gttattgagg aaaaaagtaa ggcagctagt aggctttgaa ataacgtttt gcggttggct
     8641 gatgcaattt cgagagtgca tgtattgaat gatttctagc cgttactgat tttcgtattt
     8701 tttgtatggg aggatttgca cctcccccca ccgagcctcc atgactttac tccactgtct
     8761 ctagggctgc tgtgtgccag gagcggaagc aggggggcca gagactcctt aaaagttgat
     8821 agctcgtttt gcagggagac ttacacggta agcgttattc cgaagagctt aaaattgaag
     8881 cagtcaaacg ggttgttgag cgtgtttatg cgctgccatc tgattaatcg acggaagcag
     8941 ttccgtttgg tctatgatgc agttgtccgg caggcgctct gtgacgcaaa agaacgatag
     9001 gagtagcatt gaagtgaacg aggttatgct tgtcttatat tatcctctat tttaaaaaca
     9061 tcagctggtt gtgtaagagt ttttattgtt ttatcttgtt gactttgctt ttatgaaaac
     9121 agagtgattg atacaatgaa aaccttaagt gggaaagagt ttaggaaatt atttaatcgt
     9181 tatgagacag gattctatct aagtgagttt gaatacttaa aggactttga tcctttgttt
     9241 cctgaatgcg tagggaaaga attaaagttg ggttataaac gaaaagttga tgtatggtgc
     9301 gaagaggatg ctgcgctata tgcagaattt atagctacaa aagaaaggct gaaaagaggc
     9361 cggttaatcc taaaaataaa ccaagtgtgg tcgcgtgtat atcctgctta tatttatgca
     9421 attgaagaaa gaataaatgt cagggcaaag gggagagagt catttaagta tgaaactgtg
     9481 caagaaaaac aatgtaaact ttccataatg ttagatggtc tgaaagacct tgaatgtgaa
     9541 atagctatga tttcaagtga gtttgaaatt gaaaaacact ctatgcacta tttttatatg
     9601 atttttgata ttgaacgcta tataaatgac tgcagttact ttgttttttc tgctgctaaa
     9661 gttgttaaag gcgtgattca atgcattggt tacattgaca aaaataacaa agcaagttgc
     9721 gatgaatttt attcaggcag ctatgaatgc ttggatgatg atcaggaaac actgaactat
     9781 ttaaatgaat catgcgcaga gggtatagac gctgataaag atctcatcag gaagaatgag
     9841 ttattagcct tatccctgaa ggaattgtat caacttctcg tttcaaaaaa ataacaagta
     9901 atacggcgag cgatatgacg tgccatggga tatccgcacc cagcagcatg ttgtctccac
     9961 gatgtccgca gttcgctcat aatagacatt cactacagtt atagtagaag gtatgcatgc
    10021 tgctgagtgg ggaaagtgtg aaagaaaaga agacagctgc tttgtttgtc gtcacgttta
    10081 tcttcattgg ctatgcaagt cgtaatacaa ggcgggacaa aactgagaca cataaggcct
    10141 cgcaatggct tgcaaggcca cacatgtatt gagatgttaa taaaatgtag acttgtaatt
    10201 ttgatataaa tggtagagaa aatctttccc caaaataaaa acgaacgtca atgaaatcaa
    10261 acggttgaat aaagttgatt ttggctaata caaagacaag aaaataatat ttatgattaa
    10321 atatcagcga gttgaataca taatttttat atactgctgc gccacatggg ttggactgaa
    10381 gccgcagacc tgattgttaa aggtatggaa ggcgcaatca atgcgaagac cgtaacctat
    10441 gacttcgaac gtctgatgga aggcgctaaa c
//
LOCUS       DAOFSV010000151        10450 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.151, whole genome shotgun sequence.
ACCESSION   DAOFSV010000151 DAOFSV010000000
VERSION     DAOFSV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.151"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..424)
                     /gene="slyA"
                     /locus_tag="QBH27_003945"
     CDS             complement(<1..424)
                     /gene="slyA"
                     /locus_tag="QBH27_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDQ7016203.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQ"
     gene            625..861
                     /locus_tag="QBH27_003946"
     CDS             625..861
                     /locus_tag="QBH27_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDQ7016204.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            864..1721
                     /locus_tag="QBH27_003947"
     CDS             864..1721
                     /locus_tag="QBH27_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ7016205.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1721..3733
                     /locus_tag="QBH27_003948"
     CDS             1721..3733
                     /locus_tag="QBH27_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ7016206.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVTDYRHIAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3734..4255)
                     /gene="sodC"
                     /locus_tag="QBH27_003949"
     CDS             complement(3734..4255)
                     /gene="sodC"
                     /locus_tag="QBH27_003949"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDQ7016207.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4336..5232)
                     /locus_tag="QBH27_003950"
     CDS             complement(4336..5232)
                     /locus_tag="QBH27_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDQ7016208.1"
                     /translation="MVQRITIAPQGPEFSHFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5281..5520)
                     /locus_tag="QBH27_003951"
     CDS             complement(5281..5520)
                     /locus_tag="QBH27_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDQ7016209.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5623..6222
                     /gene="nemR"
                     /locus_tag="QBH27_003952"
     CDS             5623..6222
                     /gene="nemR"
                     /locus_tag="QBH27_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDQ7016210.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6259..7356
                     /gene="nemA"
                     /locus_tag="QBH27_003953"
     CDS             6259..7356
                     /gene="nemA"
                     /locus_tag="QBH27_003953"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDQ7016211.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            7437..7844
                     /gene="gloA"
                     /locus_tag="QBH27_003954"
     CDS             7437..7844
                     /gene="gloA"
                     /locus_tag="QBH27_003954"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDQ7016212.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7947..8594
                     /gene="rnt"
                     /locus_tag="QBH27_003955"
     CDS             7947..8594
                     /gene="rnt"
                     /locus_tag="QBH27_003955"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDQ7016213.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            8823..8915
                     /locus_tag="QBH27_003956"
     CDS             8823..8915
                     /locus_tag="QBH27_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDQ7016214.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(8966..9313)
                     /gene="grxD"
                     /locus_tag="QBH27_003957"
     CDS             complement(8966..9313)
                     /gene="grxD"
                     /locus_tag="QBH27_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDQ7016215.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            9648..>10450
                     /gene="mepH"
                     /locus_tag="QBH27_003958"
     CDS             9648..>10450
                     /gene="mepH"
                     /locus_tag="QBH27_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDQ7016216.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRV"
BASE COUNT         2500 a         2723 c         2588 g         2639 t
ORIGIN      
        1 cctgtaactc aatgatatta tgctcaagtt ttgcgatgag cttaatcagt tgttccagtt
       61 cctctgcgga gatgccatgt aaaatttccg cgcgggtttt gttaataaca gcctccattt
      121 cgctgatcag tggctctgcc ttttccgtca gtttaatacg tttagcccga cgatcgctgg
      181 cacaagtttg acgcgaaatt aacccttttt cttccagttg gtccagcgta cggaccagtg
      241 atggctgctc gatgccaatc gcttttgcca gttgaatttg cgactggtct ggaggtaact
      301 gatggatatt gtgtaacgta acccaatggg tttgtgttaa ctccagcggt ttcaggcgat
      361 ggtctatcag agcacgccat atgcgcacca accgtgccag atcagaacct agtggcgatt
      421 ccaatttcat ctccttataa ttagcttgct aagatattat gcggctttta gaatagtgtg
      481 cagcaattgt attgctaaaa caaatgtatt gctgcatttg gttaccgtca gacatatttt
      541 tcagaaattg cgcgtaaatt tttcgcactt aaagaatatt tattaatcta acgcaatata
      601 ttctgtcgta aaggaatcta ctttgtgaag tttatgctca atgcaacagg attgccctta
      661 caagatctgg tgttcggtgc gtccgtctac tttcctccgt ttttcaaagc cttcgcgttt
      721 ggattcgtca tctggcttgt catacaccgc ctgcttcgtg gctggatcta cgctggtgac
      781 atctggcatc ccttgttaat ggatttatcg ctgtttgcga tttgcgtttg ccttgctctg
      841 gcaatactga ttgcgtggta actatgtcaa ttaaaacaat taagtatttc tcaacaatca
      901 ttgtagcggt agttgcggtt cttgccggat ggtggctgtg gaattattac atgcaatctc
      961 cgtggacacg cgacgggaaa atacgcgcag aacaggtttc tatcacccca caggtgtcgg
     1021 gacgtattgt tgagctgaat ataaaagaca accagctggt taacgcgggg gatcttctgc
     1081 tcacgatcga taaaacgccc tttcagatcg ccgagctgaa cgcacaggct cagctggcga
     1141 aagcgcaatc tgacctcgca aaagccaata acgaagcgaa tcgtcgtcgt catctctcac
     1201 aaaattttat ttctgccgaa gaactcgata ccgctaacct caatgttaaa gcgatgcagg
     1261 ccagcgttga tgccgcacag gcgacgctga aacaggcaca atggcaactg gcgcaaacgg
     1321 aaattcgcgc cccggtgagt ggatgggtga ctaatctcac tacccgcatc ggcgactacg
     1381 ctgacaccgg gaaaccgctg tttgcccttg ttgacagcca ctcgttttac gtcattggtt
     1441 attttgaaga aaccaagttg cgccatatcc gcgaaggtgc accagcacaa attaccctct
     1501 atagtgataa caaaacgtta cagggtcacg tgtccagtat cggtcgggcg atttatgatc
     1561 agagcgttga aagtgactca agccttattc cggatgtgaa acctaacgtc ccctgggttc
     1621 gcctcgccca acgcgttccc gtccgcttta cgctggataa agtcccaggc gatgtcacgc
     1681 tggtgtcggg caccacctgt agcattgccg taggtcaata atgaacgcat cgtcatggtc
     1741 cttgcgcaat ttgccctggt tcagggccac gctggcgcaa tggcgttatg cgttacgcaa
     1801 taccattgcc atgtgtctgg cgctgacggt tgcctattat ttgaatctgg atgaacccta
     1861 ttgggcgatg acctcggctg cggtggttag ctttcccacc gttggcggtg tcatcagcaa
     1921 aagcctcgga cgcatcgctg gcagtttgct cggagccatt gcagcactgc ttcttgccgg
     1981 acatacgctc aatgagccgt ggttttttct attgagcatg tcggcgtggc ttggcttttg
     2041 tacctgggcc tgtgcgcact tcacgaataa cgtcgcgtat gcatttcaac tggcgggcta
     2101 cacggctgcc atcatcgcct ttccgatggt taatattact gaggccagcc agctgtggga
     2161 tatcgctcag gcgcgtgttt gcgaggtgat agtcggtatt ttgtgcggcg gcatgatgat
     2221 gatgatcctg ccgagcagtt ccgatgctac agccctttta accgcattga aaaacatgca
     2281 cgcccgatta ctggaacatg ccagtttact ctggcggcct gaaacaaccg atgccattcg
     2341 tgcagcacat gaaggggtga ttgggcagat actgaccatg aatttgctgc gtatccaggc
     2401 tttctggagc cactatcgtt ttcgccagca aaacgcacgc cttaatgcgc tgctccacca
     2461 gcaattacgt atgaccagtg tcatctccag cctgcgacgt atgttgctca actggccctc
     2521 accgccagat gccacgcgag aaattctcga acagctgctg acggcgctcg ccagttcgca
     2581 aacagatgtt tacaccgtcg cacgtattat ctccccacta cgcccgacca acgtcaccga
     2641 ctaccggcac atcgccttct ggcagcgact acgttatttt tgccgccttt atctgcaaag
     2701 tagtcaggaa ttacatcgtc tacaaagcgg tgtagatgat catgccagac tcccacggac
     2761 atccggcctg gctcgtcata ccgataacgc cgaagctatg tggagcgggc tgcgtacatt
     2821 ttgtacgttg atgatgattg gcgcatggag tattgcttcg caatgggatg ccggtgccaa
     2881 tgctttaacg ctggcagcaa ttagctgcgt actctactcc gccgtcgcag caccgtttaa
     2941 gtctttgtca ctgctgatgc gcacgctggt gttactttcg ctattcagct ttgtggtcaa
     3001 atttggcctg atggtgcaga ttagcgatct gtggcaattt ttactgtttc tctttccact
     3061 gctggcgaca atgcagcttc ttaaattgca gatgcccaaa tttgccgcac tgtgggggca
     3121 actgattgtt tttatgggtt cgtttatcgc tgtcactaat cccccggtgt atgattttgc
     3181 tgattttctt aacgataatc tggcaaaaat tgttggcgtc gcgttggcgt ggttagcgtt
     3241 cgccattctg cgtccaggat cggatgctcg taaaagccgc cgccatattc gcgcgctgcg
     3301 ccgggatttt gtcgatcaac taagccgcca tccaacactg agtgaaagcg aatttgaatc
     3361 gctcacttat catcacgtca gtcagttgag taacagccag gatgcgctgg ctcgccgttg
     3421 gttattacgc tggggtgtag tgttgctgaa ctgttctcat gttgtctggc aattgcgcga
     3481 ctgggaatcg cgttccgatc cgttatcgcg agtacgggat aactgtattt cactgttgcg
     3541 gggagtgatg agtgagcgtg gcgttcagca aaaatcactg gcggccacac ttgaagaatt
     3601 acagcggatt tgcgacagcc ttgcccgtca tcatcaacct gccgcccgag aactggcggc
     3661 aattgtctgg cggctgtact gctcgctttc gcaacttgag caagcaccgc cgcaaggtac
     3721 gctggcctct taattactta attacaccac aggcatagcg ttcaccgcca ccgcccagcg
     3781 gtttgggttg atcggacata ttatcgccgc caacgtggac tatcagcgct ttgtctttga
     3841 cttcatccag tgatttcaga cgaggcgcga tgacggcatc ggtcgccttg ccgtcattat
     3901 tgactaccag tgcaggcaga tcgcctaaat gcccggcacc ttctggccct tcatgtttac
     3961 cggtattttg tggatcaaga tgcccgcctg cggattctgc ggcgctggct ttgccatctt
     4021 tggtggctgg ctggcagctt cctttggcat gaatatggaa gccatgttca ccgggaggta
     4081 atgctttcag atcgggcgaa aactccagac ctttatcggt ttcagtaatg gtgacgctac
     4141 caattgactg cccaacccct tgcgacgtga cgaggttcat ctcgactttt tcactggcag
     4201 cttgtgcgcc ggtcgcaaca accagcgcca gaatggccag actaaaacgt ttcataggac
     4261 ctccgttcat tgtgcattcc tgaataagtg taaaccagtg acacattttt gaacggcggc
     4321 tattcctaaa agtgcttacg gtacgtcgta acctagtgcc gctttacgga tacggaacca
     4381 ttgttgacgg gtcattttca gtgtttctgc ttcgactgct gcccgtacgc gctcaatttt
     4441 acctgagccg ataattggca gcggctgcga cggtaaacgt aatacccagg cgtaaaccac
     4501 ctgttcaatc gagcccgcgt ttaactcctc tgccaccaca gccagttcat cacgcagcgg
     4561 ctggaaataa tcatcattaa acagacgacc accaccaagg caggaccacg ccatcggacg
     4621 aacacgcagt tgttgcagtt ggtcgagcgt gccatccagc agtaacggct gatgcaccgg
     4681 ggatatttcc acctgattag tggcaagggt aaatggcagg cgtgattgca acagcgcaaa
     4741 ttgcgcaggt gtaaagttcg atacaccaaa atgacgcact ttgccgctct gatgcagatg
     4801 tttaaaggcg tccgccactt catcggcatc cattaacggg tctggtcggt ggattaacag
     4861 cagatccaga tgatcggtcg cgagattaat tagcgactgt tcggcgctct taatgatgtg
     4921 atcgcggtca gtgatgtaat ggccaatgac gttttcttca cacgcggtcg tcgcgatacc
     4981 gcatttactg acgatttcca tccgttcacg caggtgaggt gccagtttca gcgcctcgcc
     5041 aaacgccgct tcgcactgat agccaccata aatatcagca tggtccacgg tggtcacgcc
     5101 gagatccaga tgctcttcaa taaaactgac caactggcgg gcggacatgt tccagtccat
     5161 caatcgccag tagcccatca caaaatggga aaactccggg ccttgcggcg caatagtaat
     5221 acgctgaacc ataatcgctt cctcttatca gatatgagag gagtatacgc aagattaggc
     5281 tcaaaagagt gatggttgct ccggttcgtc tgatgacgct ggcttatttg cacgtaattt
     5341 gcgcattaat cgctgccgac aaaggcgcaa cacttcttgt ttttcgccat cgctcatttt
     5401 attccagtta aaacgctcat cccggctacg aaaacagccg cgacaaaatc cccgttcgtc
     5461 agactggcaa attccccggc acgggctctg gacgggaaag aactctaatt gctccgtcac
     5521 ctcgccctcc tcagataaga ttattaccat tattgaagct gttaatgtcc aaagtagcaa
     5581 ctttgcttgc actagaccga ctggtctact acactccaac gcatgaacaa acacaccgaa
     5641 catgatactc gcgaacatct cctggcgacg ggcgagcaac tttgcctgca acgtggattc
     5701 accgggatgg ggctaagcga actcctaaaa accgctgaag tgccgaaagg gtccttctat
     5761 cactactttc gttctaaaga agcgtttggc gttgccatgc ttgagcgcca ttacgccgca
     5821 tatcaccagc gactgactga gttgctgcaa tccggcgaag gtaactaccg cgaccgcata
     5881 ctggcttatt accagcaaac actgaaccag ttttgccaac atggaaccat cagtggttgc
     5941 ctgacagtaa aactctctgc cgaagtgtgc gatctgtcag aagatatgcg cagcgcaatg
     6001 gataaagggg ctcgcggcgt gatcgccctg ctctctcagg cgctggaaaa tggccgtgag
     6061 aaccattgtt taaccttttg tggcgaaccg ctgcaacagg cacaagtgct ttacgcactg
     6121 tggcttggtg cgaatttgca ggccaaaatt tcgcgcagtt tcgagccact ggaaaacgcg
     6181 ctggcccatg taaaaaacat tattgcgacg cctgccgttt agcaggcatt ttttatcacc
     6241 agacgaccgg gagcctttat gtcatctgaa aaactgtatt ccccactgaa agtgggcgcg
     6301 atcacggcgg caaaccgtat ttttatggca ccgctgacgc gtctgcgcag tattgaaccg
     6361 ggtgacatcc ctaccccgtt gatggcggaa tactatcgcc aacgtgccag tgccggtttg
     6421 attattagcg aagccacgca aatttctgcc caggcaaaag gatacgcagg tgcacctggc
     6481 atccatagcc cggaacaaat tgccgcatgg aaaaaaatta ccgctggcgt tcatgctgaa
     6541 aatggtcata tggccgtaca gctgtggcac accggacgca tttctcacgc cagcctgcaa
     6601 cctggcggtc aggcaccggt agcgccttca gcacttagcg cgggaacacg tacttctctg
     6661 cgcgatgaaa atggtcaggc gatccgtgtt gaaacatcca tgccgcgtgc gcttgaactg
     6721 gaagagattc cgggtatcgt caatgatttc cgtcaggcca ttgctaacgc gcgtgaagcc
     6781 ggttttgatc tggtagagct ccactctgct cacggttatt tgctgcatca gttcctttcc
     6841 ccttcttcaa accatcgtac cgatcagtac ggcggcagcg tggaaaatcg cgcgcgtctg
     6901 gtgctggaag tggtcgatgc cgggattgaa gaatggggtg ccgatcgcat tggcattcgc
     6961 atttcgccaa tcggtacttt ccagaacacg gataacggcc cgaatgaaga agccgatgca
     7021 ctgtatctga ttgaacaact gggtaaacgc ggcattgctt atcttcatat gtcagaacca
     7081 gattgggcgg gaggtgaacc atatactgat gcgtttcgcg aaaaagtacg cgcccgtttc
     7141 cacggtccga ttatcggcgc aggtgcatac acagtagaaa aagctgaaac gctgatcggc
     7201 aaagggttaa ttgatgcggt agcatttggt cgtgactgga ttgcgaaccc ggatctggtc
     7261 gcccgcttgc agcgcaaagc tgagcttaac ccgcagcgtg ccgaaagttt ttatggtggc
     7321 ggtgctgaag gttacactga ttacccgacg ctgtaatcca acattgcgag cggcgcaaag
     7381 ccgccgctat actaaaacaa ctttttgaat ctgttagcca ttttgaggat ataaagatgc
     7441 gtcttcttca taccatgctg cgcgttggcg atctgcaacg ctccatcgat ttttatacca
     7501 acgtgctggg catgaaactg ctgcgtacca gcgaaaaccc ggaatacaaa tactcactgg
     7561 cgtttgttgg ttacggcccg gaaaccgaag aagcagtgat tgaactgacc tacaactggg
     7621 gcgtggataa atacgaactc ggcactgctt atggtcacat cgcgcttagc gtagataacg
     7681 ccgctgaagc gtgcgaaaaa atccgtcaaa acgggggtaa cgtaacccgt gaagcgggtc
     7741 cggtaaaagg cggtactacg gttatcgcat ttgtggaaga tccggacggt tacaaaattg
     7801 aattaatcga agagaaagac gccggtcgcg gtctgggcaa ctaatctcct gccgggcgtg
     7861 aactcatcgc gcccgcatct ttactgcatc gacaagtaat atttgtcata atgcgcgctg
     7921 caatttatcc gtattaagag aatcagatgt ccgataacgc tcaacttacc ggtctgtgcg
     7981 accgttttcg tggtttttat cctgttgtga tcgatgttga aacagccgga tttaacgcca
     8041 aaaccgatgc gctgcttgag attgccgcca tcaccctgaa aatggatgaa caaggctggc
     8101 tgatgccgga caccacatta catttccacg tcgaaccatt tgtcggcgca aatttgcaac
     8161 cagaggccct tgccttcaat ggcattgatc cgaacgatcc cgatcgcggc gcggtcagcg
     8221 agtacgaggc gctgcacgaa atttttaaag ttgtacgtaa aggtattaaa gcgagcggct
     8281 gtaaccgcgc cattatggtg gcgcacaacg ccaatttcga tcacagcttt atgatggccg
     8341 ccgcagaacg cgcctcactg aaacgtaacc cgttccaccc tttcgccact tttgacactg
     8401 ctgcactggc cgggctggca ctcggacaaa ccgtattgtc aaaggcttgc cagacagctg
     8461 gcatggactt cgacagcacc caggcgcact ccgcgctgta cgacaccgaa cgcactgctg
     8521 tgctgttttg tgaaatcgtc aaccgctgga aacgtctggg aggctggcca ctacctgccg
     8581 ccgcagaggt gtaatcgagt cgatgcctga tgacatgcaa tgattcaggc atctatagtg
     8641 aggctattcc acgcatcatg catgatattc acggggaata gcgttaatgg caaataatcc
     8701 aaacccttca tcgcccctgc cggacgtgtt ttcgccggcg acccgcgact ggtttcttcg
     8761 cgcctttaaa cagccgaccg ctgtctagtc gcgaaccacg gctgcgcttt acgctaacag
     8821 aagtgaatga cctaccggtc cggcaaacgc cgatgtttac gctgttacgt gaggcgggat
     8881 tttcaagttc gccacaaggg ctggattggg gatagagaaa ggactgacgg atgcccgttc
     8941 gcatccgtca gtattgcagg acggattatt ccgcgtccgg ctcttcagac ttgtatttag
     9001 cggcagtttc tttgatcagc tgctgcagtt caccacgctg atacatttcg atcacgatat
     9061 cacaaccgcc caccagctcg ccgtcaaccc acagttgcgg gaaggtcggc cagttcgcat
     9121 atttcggcag ttccgcacga atgtccggat tctgcagaat atcaacatag gcaaaacgtt
     9181 cgccacatgc ggcaagcgcc tggactgcct gggcagagaa accgcagctc ggcagtttcg
     9241 gtgaaccttt catgtacagc aggatcgggt tttcagcaat ctggcgttgg attttttcga
     9301 tagtggtgct cattgtcttg cttcctcaaa cttctttacg gcagtaatct gacattgtag
     9361 cgggtcagtg cggcatcgga aaataacatt ttcatcacgc ttttgctatt ttatcccttt
     9421 gctctatttg ttgcatttca aatattcgtt tcttccctga tgcacggcaa actggcgtat
     9481 ttataaccat ttttagcttt cactgctgca tttttttgcg ctcgccgacg aaacgtattt
     9541 tttaacaata aaagctatta actttctctt cttctatgca ttagaatcat caagttttgt
     9601 aaatcagacg caggcatgat agacctgcct taacagaggg acgctcagtg gcgcggataa
     9661 accgtatttc gatcacgctc tgtgctttac tttttaccac cctgccttta acgcctatgg
     9721 cccatgcttc aaagcaagcc agggagagtt ctgctaccac tcatatcacc aaaaaagcag
     9781 ataaaaagaa aagcacggca accacccaaa agacccagaa agccagccag aaaacagcga
     9841 aaaaagccgc cagtaaaagt acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt
     9901 ccattaccgc ttctaaaaac gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct
     9961 cagccagctt taccgaaaag tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag
    10021 tcaaaaatgc cgcgtcagga acccttgccg acgcgcacaa agcgaaggtg caaaaagcca
    10081 caaaagtggc aatgaataaa ctgatgcagc aaattggtaa gccatatcgt tggggtggca
    10141 gctcaccgcg taccggtttt gattgcagcg gtctggttta ttacgcttat aaagatttgg
    10201 tgaaaattcg tattccgcgt acggcgaatg aaatgtatca cctgcgcgat gcagcgccaa
    10261 tcgaacgtag tgaactgaaa aacggcgacc tggtcttctt ccgtactcag ggacgcggta
    10321 cagccgatca tgtcggcgtg tatgtcggca acggcaaatt tattcagtca ccgcgcacgg
    10381 gccaggaaat tcaaatcact tctctcagtg aagactactg gcagcgccac tatgttggcg
    10441 ctcgccgggt
//
LOCUS       DAOFSV010000152        10404 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.152, whole genome shotgun sequence.
ACCESSION   DAOFSV010000152 DAOFSV010000000
VERSION     DAOFSV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.152"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(20..2647)
                     /gene="gyrA"
                     /locus_tag="QBH27_003959"
     CDS             complement(20..2647)
                     /gene="gyrA"
                     /locus_tag="QBH27_003959"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDQ7016217.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            2794..3516
                     /locus_tag="QBH27_003960"
     CDS             2794..3516
                     /locus_tag="QBH27_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDQ7016218.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(3656..7414)
                     /gene="yfaL"
                     /locus_tag="QBH27_003961"
     CDS             complement(3656..7414)
                     /gene="yfaL"
                     /locus_tag="QBH27_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDQ7016219.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDKT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGIAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGRWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNRGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YAWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQMRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            8096..10381
                     /gene="nrdA"
                     /locus_tag="QBH27_003962"
     CDS             8096..10381
                     /gene="nrdA"
                     /locus_tag="QBH27_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDQ7016220.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         2489 a         2868 c         2467 g         2580 t
ORIGIN      
        1 ttggcatgaa gcgatgaaat tattcttctt ctggctcgtc gtcaacgtcc acttccggag
       61 cgatttcatc gtccccttcc gcggcactgc cgtcgatggt atccagatct tcctcgtcaa
      121 ccggttcagc aacacgttgc agacccacca cgttttcatc ttccgaagta cggataagga
      181 ttacgccctg ggtgttacgg cctacgatgc tgatttccga cacgcgagta cgtaccagcg
      241 taccggcgtc ggtgatcatc atgatctggt cgcaatcatc tacctgcacc gcgccaacca
      301 ctaaaccgtt acgttcggta accttgatgg agataacccc tttcgtcgca cgcgacttgg
      361 ttgggtattc cgccactgcg gtacgtttac cataaccgtt ctgcgttgcg gtgaggattg
      421 cgccatcgcc acgaggcacg atcagagaga cgactttatc gccttcgcct aaacgaatac
      481 cacgaacacc ggtggtgttg cagcccatcg cacggacaga agactcttta aagcgcacca
      541 ctttaccttc agcggagaac agcattactt cgtcttcgcc gctggtcagg tcaacgccga
      601 tcagctcatc gccgtcaacc agtttgatcg ccactttacc ggcggtacgc agacggttga
      661 actcggtgag gacggttttc ttcacagtac cgttagcggt cgccatgaag actttcacgc
      721 cttcttcaaa ctcggtcacc ggcaggatcg cggtgatacg ttcgtcctgt tccagcggca
      781 gcaggttgac gatcggacga ccacgtgcgc cacgagtagc ttctggcaac tggtagacct
      841 tcatcgaata gacgcggcca cggctggaga agcacagaat atggtcgtgg gtgttcgcca
      901 ccagcagtcg gtcaataaag tcttcttctt taatacgtgc ggcagattta cctttcccgc
      961 cacgacgctg cgcttcgtat tcagaaagcg gctgatactt cacgtaaccc tggtgagaaa
     1021 gcgtgaccac cacatcttcc tgtgtgatca gatcttccag gttgatgtcg gcgctgttag
     1081 cggtgatttc ggtacgacgt ttgtcaccga actgttcacg aaccagctcc agctcttcac
     1141 ggatcacttc catcagacga tcggcgctac ccagaatacg caacagctcc gcaatctgat
     1201 ccagcagctc tttgtattcg tcgagcagtt tttcgtgctc aaggccggtc agtttctgca
     1261 aacgcagatc cagaatcgcc tgagcttgct gttcggtcag gtagtacaga ccatcacgca
     1321 cgccgaactc tggctccagc cattccggac gcgcagcatc atcgcccgca cgttccagca
     1381 tcgcagcaac gttgcccagc tgccacggat tagcaaccag cgcagttttc gcttctgcag
     1441 gcgtcggcgc atgacggatc agttcgatga tcgggtcgat gttcgccagc gccacggcta
     1501 atgcttcaag aatatgagca cgatcgcgag ctttacgcag ttcgaaaatg gtacgacggg
     1561 tcaccacttc acggcggtga cgaacaaacg ccgcgatgat gtctttcagg ttcatgatct
     1621 tcggctgacc atggtgcaat gccaccatgt taataccgaa agaaacctgc aactgggtct
     1681 gggagtagag gttgttgagc accacttcac cgaccgcatc gcgtttgact tcaatcacga
     1741 tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct gatgccttcc acgcgttttt
     1801 cttttaccag ttccgcaatt ttttcgatca ggcgcgcttt gttcacctga tacggaattt
     1861 cgtggacgat aatggtttca cgtccggttt tggcgtcaac ttctacttcg gcacgggcac
     1921 ggatatatac cttgccgcga ccggtacggt aagcttcttc aataccgcga cgaccgttaa
     1981 tgattgccgc cgtcgggaag tccggccccg ggatgtgttc catcagccct tcaatgctga
     2041 tgtcttcatc atcgatatac gccagacaac cgttgatgac ttccgtcagg ttgtgcggcg
     2101 gaatgttggt tgccatacct acggcgatac cggaagaacc gttcaccagc aggttaggaa
     2161 ttttggttgg catgacgtcc ggaatttttt ccgtgccgtc atagttatca acgaaatcga
     2221 ccgtctcttt ttcgagatcg gccatcagtt catgggcaat tttcgccaga cggatttccg
     2281 tataacgcat tgccgccgca gagtcgccgt cgatggaacc gaagttaccc tgaccgtcta
     2341 ccagcatgta acgcagcgag aatggctgcg ccatacggac gatcgtgtta taaaccgcca
     2401 agtcaccatg gggatggtat ttaccgatta cgtcaccaac gacacgggca gattttttat
     2461 aggctttgtt ccagtcattg cctagtacgt tcatggcgta aagtacgcga cggtgtaccg
     2521 gcttcaggcc atctcggaca tctggcagcg cacggccaac aatgaccgac atcgcataat
     2581 ccagatagga gctcttcagc tcttcctcaa tgttgaccgg tgtaatttct ctcgcaaggt
     2641 cgctcatcta accgctatcc ctctactgta tcccggattc aaaggtcgca aattataaca
     2701 cagccgcgca gtttgaggta aacctatacg ctttattcac atccaatgcc tgatatactc
     2761 gtttgtcttg ccaattacgg agtagaagtg ccaatgaatg ccgaaaaatc gccggtaaac
     2821 cacaacgtag accacgaaga gatcgctaaa tttgaagccg tcgcctcccg ctggtgggat
     2881 cttgaaggtg agttcaaacc gctgcaccgc attaacccgc tgcgtctggg ctatattgcc
     2941 gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg tcggttgtgg cggcggcatt
     3001 ctggccgaga gtatggcgcg ggagggcgcg acggtgaccg gtctggatat gggctttgag
     3061 ccattgcagg tggcaaaact gcacgcactg gaaagcggca ttcaggtgga ttacgtgcag
     3121 gaaaccgtgg aagagcacgc ggcaaaacat gccggacagt atgacgtggt gacctgtatg
     3181 gagatgctgg agcacgtccc cgatcctcag tcagtggtca gagcctgtgc gcaactggtg
     3241 aaaccgggcg gcgatgtctt tttctcgacg cttaaccgca acggcaagtc atggctgatg
     3301 gcggtggttg gtgctgaata tattttgcgc atggtgccta aaggtacaca tgatgtgaag
     3361 aagtttatta aaccggcaga attgctgggc tgggtggatc agaccagtct taaagaacgg
     3421 catatgaccg ggctgcatta caaccctatc actaacacct ttaagctcgg ccccggcgtg
     3481 gatgtgaact atatgctgca tacgcagaat aagtgaggtt gttgtttgga ttcatccggc
     3541 aaccgcgcca agcctgatgc gacgcttgcc gcgtcttatc aggcctacaa atgctccccg
     3601 taggccagat aaggcgttta cgccgcatcc ggcaacagtg ccggtcagac agcgattacc
     3661 atttcactgt catcgacaaa aaccctgcca tctgggcaaa atcatcactc cctttctgcc
     3721 acgccacgct gccgcgcagc gaaactcgct gactgatatt gcccgttatt cccactttta
     3781 tttcaccccg ttgcttcacc gcatcgtcac tgatagtgct gccatcctct tcaatttccg
     3841 tcgcatgggg atcgtgataa taattcagat ctaacgttgg cgtgacacca acagcagtac
     3901 gccattcgct gtgtaaaccc aaccgcatct gaatatcatc accctgcgat tgtgacacgc
     3961 gcgcatggtt agcggcggta aaatcatcct gctgcacgcc ctgataaatc acctgcgcct
     4021 gcggttcaat caccacacca cgccccggta accactgata accggcttcc agcgaggcga
     4081 taatccccga cgagtggtaa tgatccactc catcgtcttg ttcggaaaca tcattgttaa
     4141 accacgcata ttgcagccag ctatccaacc aggccccctg cttctgatta ccgtgctgat
     4201 accagcttga ggtcagccca acggcataac cgcggttctg gttatcggcg cgggttccgg
     4261 tcatattcga gcggctgtcg ccctggttat cgctgtagcc accaacaatc ccaagcatcc
     4321 actcgccatc atcaccccag cgcccgctaa acaggttgcc gctaagctgc accgtagaag
     4381 tgtcttcatg ctgagccaac tgccctgccg ccgtgtaatg ataacgcccg ccgataacgc
     4441 gtaaattcag cgtctgacca tcgccacctg cgtgatcgcg tcgctccatc acaaatgcct
     4501 gatttgccgc acgtaggtta ttaaaatatc caccaacttt ggcattcaac accggctggt
     4561 aagcaggcgt aggttcaggg tcgggggttg ggtcgggatc cggcgtggga tcaggatctg
     4621 gagtcgggtc tggatcaggc ggcggcgtcg gattgacttc ttgtgatcgc agataccagt
     4681 cgttgttatc ttccaccagc gtgtagtcat acgcccccat attgacgtag ccgcttcctg
     4741 ccagactgaa ctgcgcattg ttttgaaact gcgtgggatc agctgcgaaa tcaaccactt
     4801 taatacctgt agatgtcggc tcaccaatcc ctgtaatggg attaatcaca accgtggtat
     4861 tgccagcagt attaccgttc aacaccaact ggtcgcttgc cgagtcatcg ccgtttaatt
     4921 cgctgtcgag cagtagcgta ccgccgccgg tataatcacc atttaccgtc agcgtgtcgc
     4981 cagcgatgcc attttgcaga ctgacagcac cggacgtatt ggtcagattg ccattaaccg
     5041 tcacgtcgga aagataagtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca
     5101 acgtcacgcc atcggtggca ttcaccagcg taccgtcgcc gctaaacagc gaggcatcca
     5161 gcgcctggtt cgtatcctgc tgcgtcaatc gcaacacagt accatcggtt atgtcgatat
     5221 cgccgccaga ggtggcatcg attgagcgaa tatcctgatt ttgcccggtt tcaaaaacac
     5281 cgtcttcccc cacccacagt gaagaaacat aaggaataat attctccgct tcacctttta
     5341 gcgttccgtc ttccacacgt accgcagacg tcgcagtgcc gctggcggtc aactccagcg
     5401 tcccgccgcc acttttagtc aacgtgctta cgctatccag ttgttgcccg gtactatccg
     5461 ccatcaaccc gccccattgc gtatctacgc catcctcaac gtgtatttcg ccatcagcgc
     5521 gcatttcaat attcctgccc tgacctgttg tagccgagcg agtgtcaaca tcagtcgtca
     5581 catccatttc actgtgttgt tggggatctg tgaagatcac tgaacggttg taacttgtgt
     5641 cccccaattg cgaattatcg ctgacttcaa gcgtaccgga ggcgatttgc gtattaccga
     5701 gataaccgtt gttattcgcc agcgttacgc gctgatcccc cgcattaatg acgacataaa
     5761 cgccattctg gctggcatca ctgccgccaa tatgtaaatc atcgccactg ctgatatcaa
     5821 cgacatcatt actgccaatc actgtccccc cgctgccgct gatttgcaga ccgtcattgt
     5881 atgagctatc gccatcccag gtggtgaaat cagagagatc caatactccg ccatttagca
     5941 cgatcgattg cggatcgctc tgcattgccc gtaaatcggc ctgattcccc gccagcgaca
     6001 atacggcgtt atcttccacc acaatatcgc cggttaatgc catcgactgc gcccctgtca
     6061 ggagatagct gccatcttgc gctaccgtca actgtccttc accctgaatc gagccggaaa
     6121 aaccgccctg gtttacggta acattgccgc ctgcgtcgat attcaaaatg ccattagcaa
     6181 aaccggttaa tgagtgcaca aacgtttgtt gtgtattgcc aacattcagt tctgcctgat
     6241 actcagaatg aacagtgctg ccgaccatca gaccaaagca atcctgcgga tcgctctgac
     6301 agtgcgtgtc gccgacattc attaactcat cgctgcgccc caacgtcacc tcgccattct
     6361 ggatactcat ctcgccggta aacgcattat tgtcggcatt gaggactaac tctcccgctc
     6421 cctctttcac gatgactccc gttccggcaa gagaatcaat cgcgccatcg ttgctggtat
     6481 tgcctattac cagcgtttta tcggcggcaa tgttaaattc agccacacta tgaccgatat
     6541 acataaagcc ccccgccgct gccgtcgccg aatggtcata tcccgaggcg ctgttatttt
     6601 catcaatcat cacaccattg ttttggtcat aattatcatc aacagaaata tcaataagat
     6661 acggggatgt ggtgctgctg gtataaattg ctccgccata accttctgca tagttattgg
     6721 taaaggaggt attattaatt atcgtttttc ctgaaagatg tgtcttatcc gtactattat
     6781 ccgtaacatc aatggcgccg ccatccccat cactataact tgttgatgta tatgcctggt
     6841 tattattaaa agcatcatca cttaaataga cgtcattatt gatggtataa attgcaccgc
     6901 ctttgccgtc attagcgatg ttattgtgaa atacggcgtt agtgactcgt aaatcggcgg
     6961 caccggtatc attggtaccg gcagaataga ttgctccgcc atagcctcct gcaacattac
     7021 tgtcgaaaat aacattcgca agatttatcg ttgagttttc tttagcaaag atagccccgc
     7081 cattattata ttcgccacta accgagttat cagcaaacag ggtcatccct gtttcatttt
     7141 gtggtgatat cgtaaattca gcactgtgct gcaaaaatac tgcgccaccg ctggcattat
     7201 tgaccatacc cgaaaaaatc aaccattgtc catccgcgat gctccagtcc tgtatattac
     7261 ccgaaaggct ttgcctgctg gcctgacagc tcgctttgat atcatatccc tggcatgaat
     7321 caatgaccgc tgcggcaccg ttagcagaga aaagagatgc aatcattgac gggagtaaag
     7381 ataaatactc cttgcgtaga gagataatcc gcatattcat ctaaccatca ttttctataa
     7441 gatggcgtat ttaatcgcat tatgcgatat agggtatttt tcttttgtaa attctgcccc
     7501 cgatgtaata accactttgt tattatttga tgattaattt agatgcatca caattcagat
     7561 taataacttt aaggtaagtc ggttatattt aaactgaatt aaagcaataa ataatattct
     7621 tataatatat gcaaaagtaa caaattggct tagtatttaa caataaccga atataaaaca
     7681 accatttccg actcaacaat cgcttacatt cgcttatata ttgaccacaa cagatacatc
     7741 agattatgtg acgacttgtg cctagatcaa ttttggcaat cattagcaac aagattaata
     7801 aaccatctat atcaatttac ctatcctatt ttaccgttca agaattcacc gaacggttat
     7861 ttttaacaaa tttttctctt cccattgact ttcccggaca ccttgtctga cctaaggtgc
     7921 gcggaagcca ctttttcctt cctgagttat ccacaaagtt atgcacttgc aagagggcca
     7981 gtttcacact atcttgcagt gaatcccaaa cataccccct atatatagtg ttcttagcag
     8041 cttccctcac tacaggtagt ctgcatgaag ctattgaaaa acaggtacga catacatgaa
     8101 tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc tcgacaaaat
     8161 ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt cccaggtcga
     8221 gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc acgaaaccat
     8281 tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt atctcgccgc
     8341 acgtctggcg atcttccacc tgcgtaaaaa agcctacggt cagtttgagc cgcctgcgct
     8401 gtacgaccat gtggtgaaaa tggtcgagat gggcaaatac gataatcatc tgctggaaga
     8461 ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc gtgatatgac
     8521 cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga accgcgtgac
     8581 cggcgaaatc tacgaaagcg cccagttcct ttatattctg gttgccgcgt gcctgttctc
     8641 gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg cggtttccac
     8701 atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga ctcgtcagtt
     8761 cagctcatgc gtgctgatcg agtgcggcga cagcctggat tccattaacg ccacctccag
     8821 cgcgattgtt aaatacgttt cccagcgtgc cgggatcggc atcaacgccg ggcgtattcg
     8881 tgcgctgggt agcccgattc gcggtggcga agcgttccat accggttgta ttccgttcta
     8941 caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg gcggtgcggc
     9001 aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt tgaaaaacaa
     9061 ccgtggtgtg gaaggcaacc gcgtgcgtca tatggactac ggggttcaaa tcaacaaact
     9121 gatgtatacc cgtctgctga aaggtgaaga tatcactctg ttcagcccgt ccgacgtacc
     9181 ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgtctgt ataccaaata
     9241 tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt tctcgctgat
     9301 gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc actgcaatac
     9361 ccatagcccg tttgatccgg ccatcgcgcc agtgcgccag tctaacctgt gcctggaaat
     9421 cgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa tcgcactgtg
     9481 tacgctgtct gctttcaacc tgggcgcaat taacagcctg gatgaactgg aagagctggc
     9541 aattctggca gtacgcgcac tcgacgcgct gctggattat caggattacc cgatcccggc
     9601 cgccaaacgt ggagcgatgg gtcgtcgtac gctgggtatt ggtgtgatca acttcgctta
     9661 ctacctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc tgacgcataa
     9721 aaccttcgaa gccattcagt attacctgct gaaagcgtct aatgagctgg cgaaagagca
     9781 aggcgcgtgc ccgtggttta acgaaaccac atacgcgaaa gggatcctgc cgatcgatac
     9841 ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact gggaagcact
     9901 gcgtgagtca atcaaaacgc acggcctgcg taactccacg ctttctgccc tgatgccgtc
     9961 cgagacttct tcgcagatct ctaacgccac taacggtatt gaaccgccgc gcggttacgt
    10021 cagcatcaaa gcgtcgaaag acggtattct gcgccaggtg gtgccggact acgagcatct
    10081 gcacgacgcc tatgagctgt tgtgggaaat gccgggtaac gatggctacc tgcaactggt
    10141 gggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact acgatccgtc
    10201 acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc tcactgccta
    10261 caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgcag aagacgcgca
    10321 ggacgatctg gtgccgtcaa tccaggacga tggctgcgaa agcggcgcat gtaagatctg
    10381 ataatccccc agtctttgca ccaa
//
LOCUS       DAOFSV010000153        10397 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.153, whole genome shotgun sequence.
ACCESSION   DAOFSV010000153 DAOFSV010000000
VERSION     DAOFSV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.153"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(8..2713)
                     /gene="secA"
                     /locus_tag="QBH27_003963"
     CDS             complement(8..2713)
                     /gene="secA"
                     /locus_tag="QBH27_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDQ7016221.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEIEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(2775..3287)
                     /gene="secM"
                     /locus_tag="QBH27_003964"
     CDS             complement(2775..3287)
                     /gene="secM"
                     /locus_tag="QBH27_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDQ7016222.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLETNTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(3517..4434)
                     /gene="lpxC"
                     /locus_tag="QBH27_003965"
     CDS             complement(3517..4434)
                     /gene="lpxC"
                     /locus_tag="QBH27_003965"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDQ7016223.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(4535..5686)
                     /gene="ftsZ"
                     /locus_tag="QBH27_003966"
     CDS             complement(4535..5686)
                     /gene="ftsZ"
                     /locus_tag="QBH27_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDQ7016224.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(5747..7009)
                     /gene="ftsA"
                     /locus_tag="QBH27_003967"
     CDS             complement(5747..7009)
                     /gene="ftsA"
                     /locus_tag="QBH27_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDQ7016225.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7006..7836)
                     /gene="ftsQ"
                     /locus_tag="QBH27_003968"
     CDS             complement(7006..7836)
                     /gene="ftsQ"
                     /locus_tag="QBH27_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDQ7016226.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(7838..8758)
                     /gene="ddlB"
                     /locus_tag="QBH27_003969"
     CDS             complement(7838..8758)
                     /gene="ddlB"
                     /locus_tag="QBH27_003969"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDQ7016227.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQSAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(8751..10226)
                     /gene="murC"
                     /locus_tag="QBH27_003970"
     CDS             complement(8751..10226)
                     /gene="murC"
                     /locus_tag="QBH27_003970"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDQ7016228.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10280..>10397)
                     /gene="murG"
                     /locus_tag="QBH27_003971"
     CDS             complement(10280..>10397)
                     /gene="murG"
                     /locus_tag="QBH27_003971"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide)
                     pyrophosphoryl-undecaprenol N-acetylglucosamine
                     transferase; involved in cell wall formation; inner
                     membrane-associated; last step of peptidoglycan synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HDQ7016229.1"
                     /translation="LAGWSRETLLTMAERARAASIPDATERVANEVSRAARA"
BASE COUNT         2384 a         2959 c         2484 g         2570 t
ORIGIN      
        1 ttagctttta ttgcaggcgg ccatggcact gcttgtattt tttaccagaa ccacacgggc
       61 aaggatcgtt acgtcctact tttcgttcac cggtttgcgc cgccagtgca gctgcggctg
      121 cagagtcgtc atcctgatgg ctaagctgct gcatttgcgc taaacgctcg gcttccatac
      181 gacgctgttg ttccagctcc tcaacctctt caggcatacg cacctgaact ttgctcagcg
      241 tgctgataac ttcatatttt aacgactcca gcatcgctgc aaacatggag aacgattcac
      301 gtttgtattc ctgcttcgga tctttctgtg catagccacg caggtggata ccctgacgca
      361 gatagtccat cgcagccagg tgctctttcc acagagagtc aagcgtttgc agcatgacgc
      421 ctttctcgaa gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt
      481 cgatggactg cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat
      541 ccagccactc ggcaattggc aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc
      601 ccggaatatc ccacatttct tccagcgact gtggcggaat gtaggcatca atggtcgctt
      661 tgaacacatc ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt
      721 cgttacgctg ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca
      781 gttgcttacg aatgtcgaag ttacggcttt caactttacg ctgggcgttg gcaatcgctt
      841 tggtcaccca cgggtgctca atggcttcgc ctggcttcat acctagttta cgcatcatgc
      901 cggatactcg gtcggaggca aaaatacgca tcagcgcatc ttccatcgac agatagaaac
      961 gggaagaacc agcatccccc tgacgaccag aacgaccgcg cagctggtta tcgatacgac
     1021 gggattcgtg acgttcagta ccgatgatat gcaggccacc tgctgccagt accgcatcgt
     1081 gacgtacctg ccagtcggct ttaatttttt caatttgctc tgcagtcgga ttttccagcg
     1141 cggcaacttc tgcctgccag ctaccaccga gcacaatatc ggtaccacga cccgccatgt
     1201 tggtcgcgat agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg
     1261 catggaattt ggcgttcagg acgttgtgct taataccggc tttggtcagt tcatttgaca
     1321 ccagctccga tttttcgatg gagattgtac ccaccagcac cggctggcct ttcgcagtac
     1381 gttctttgat atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt
     1441 ccggcagatc tttacgaatc attggacggt tggtcggaac aacgacagta tccagcttat
     1501 agatggagct gaattcgaaa gcttcggtat cagcagtacc agtcatcccc gccagttttt
     1561 catacagacg gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga
     1621 tctgcacacc ttctttcgct tccacagcct ggtgcagacc atcagaccag cgacgcccct
     1681 gcatggtacg accagtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt
     1741 cgacgtcacg ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca
     1801 tgatgttggc cggagagtac agagactccc cttcatccat gatgccttct ttcaccagca
     1861 gttcttcaat cagcaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca
     1921 ccgagaagtg gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt
     1981 gcggaataat tttattcacg cgtttataca tttccgagct gtcttctgcc gggccagaaa
     2041 tgatcagcgg tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat
     2101 agtgcagttt acgctgtaca cgttcttcag gactgaacgc catgttgtca cgcaggtagt
     2161 caaagccgta ttcgttgttc gtaccgtaag tgatgtcagc agcgtaggct tcacgctttg
     2221 ccggtgctgg catgcccggc aggttgatac cgacagtcag gccaaggaat tcaaacagcg
     2281 gacggttgtt ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaacg acgtgcacgc
     2341 ctttaccggt tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac
     2401 cggtacgcat ttccgcgatg cagcgttcat taagaaccat accgccgagc agctgtacgt
     2461 cgaagtggcg catgccaaaa acacgcttac tggcttcacg caccacggcg aaggcttccg
     2521 ggatcaagtt ttccagcact tcgccttttt ccagacgcgc gcggaactct gccgtttttc
     2581 ctttcagttc ttcgtcggag agtttttcaa tctccggttc catggcattg atgatgttga
     2641 ccactttgcg catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaata
     2701 atttgattag cataataaaa tctcaaacgc cccgcgttgc ggagttaata aaatgaagta
     2761 aaggtttatt gttgttagct gaggcgttga gggccagcac ggatgccttg cgcctggctt
     2821 atccagacgg gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa
     2881 cccttttcag acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca
     2941 agatgttgcg cctgaagagg caaagattct tcagcaacgg gcagcgtttg cggtgccatt
     3001 gcaaaggaaa gatggcggat taccgtgcga atggcatgtt gatgccagta atcaacggaa
     3061 tagttcgaat tcgggcggcg cgtgttcgtt tccagcaagg ccaattgacc aaagttaact
     3121 ttggctgaag gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggca
     3181 ttgctgagtg caggcaaacc taaactcgcc gcgaccatcc ctaataagag atgcggccag
     3241 aagtagcgtt taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc
     3301 cgatcgtatt caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc
     3361 acgaatgaag ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt
     3421 atagctctta taaaattcaa aaaaaacgac tggttcacct ggccggagag agtgccagat
     3481 ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacgg ctgaaggcgc
     3541 tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg
     3601 tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt
     3661 aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt
     3721 gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc
     3781 aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc
     3841 gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc
     3901 ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat
     3961 ggtgaaatcc agcgaaaaac cattgtacgg tttaaattca gcccacttat cgccatcttc
     4021 gacacgaaca gtctctttaa tgcgaacaaa tttcttggcg cagttcaact cgtcgatacc
     4081 ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg
     4141 cgcgttaact tcgataacaa tgttatcgat gcccaagccc gcgagagcag cattgaggtg
     4201 ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc
     4261 acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata
     4321 gatgaccccg gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg
     4381 taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt
     4441 atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca
     4501 caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta cgcaggaatg
     4561 ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcacgactt
     4621 tagcaaccgg cttctgctcc tgggtcagcg gagccatacc gtgctgctgg tagcgatcca
     4681 tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc
     4741 cgatacctgt cgcaacaaca gttacgcgca gctcgtcatt catatccggg tcaagagaag
     4801 taccgataac cacggtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt
     4861 cgaactcatc cagacgcagg tcgaagcccg ctgtgatgtt taccagcacg ccgcgtgcgc
     4921 cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg
     4981 cacggtcttc accgctcgcc acaccagaac ccatcattgc gtagcccatt tcagacatta
     5041 cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcggcga
     5101 taccttgcac agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga
     5161 taccacggcc cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat
     5221 gcttggacag ttcagtgatc ccctgctccg cgaatgccat acgcttcttg ccttcaaagt
     5281 tgaaaggctt agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga
     5341 cgactggtgc tgcacctgta ccggtgccac cacccatacc cgcagcaata aagaccatgt
     5401 ctgcaccttc cagcgccgca cgcagtgcat cgcgatcctc atcagccgca ttgcggccaa
     5461 cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct
     5521 gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac
     5581 cttcaatgcg ctcacgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga
     5641 tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat
     5701 ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc
     5761 gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta
     5821 cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg
     5881 aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac
     5941 gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc
     6001 caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt
     6061 cctgcaactg caatatctct tcgttgacca gattgagcag ctcggtatag cgtggctcga
     6121 tcacctctgc cagggtctga cgttgcagac tccgcggcgg acgaccgcct acgctcggca
     6181 cttccacgct ctcatctttt ccaacgatgg agcccagcgc acaaccgtgg cgaactttaa
     6241 tcgcttcggc gtcacttggc ggtgtgccaa aggcgtaagc gatatcactg gtcacgacat
     6301 tgccagcata aggaattacc ttcgtgtggc gcaatgcccc accggtataa acggcgatat
     6361 ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca
     6421 ataccgaata acttgatgcc agaccggcaa atatcagttg gtcaactttc agcccacaac
     6481 gttcaaccgc tttgacgatg tttttcgcca tatcattgtg acatgtgatc aggtgcactt
     6541 ttgcctgcat ccgcacgccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa
     6601 tcgcatactc ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt
     6661 tcgcggtatg gacgacgttt tccacatctt cttgcgtcac ttcttcttca gaaataggca
     6721 ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag
     6781 atatctgaca atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca
     6841 cggattcgag gtcgttcacc ccgcctttat ccataccacg cgacgggcaa ctacccacgc
     6901 caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg
     6961 taccaatctc cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc
     7021 ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc
     7081 cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg
     7141 ctgctgtaaa accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg
     7201 gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgtc gcgcggtcat
     7261 cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc
     7321 ctgcaacact tcattggcgc tgccttccgg gccatacagc attggaagca cctgcttgct
     7381 ggtgcgatct ggcggcacgc tgaaggtgtt tccttccgcg tctaccatat gttgatcatt
     7441 ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt
     7501 tctgacgctc acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt
     7561 gacatcctgg gtcataaagg tgcctggctc acccaatgcc aggatcgact gccggatatc
     7621 gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg
     7681 cgcatcttcc atccagccca acacgaccca gccgctcacc aacactgtcg ttaaaacggt
     7741 cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc
     7801 ttcgctattt cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc
     7861 gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accagactgt
     7921 ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca
     7981 gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct tttagcacta
     8041 atgcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct
     8101 catcagagag atacttcgcc tcataatcat agaaggttcc ggccggttga atacgtattg
     8161 acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt
     8221 caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt
     8281 ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta acgattaccg
     8341 gcaaacccag agaagaaatt tctgctaact gcttatcgtt aaggcctttt tcaaactctg
     8401 cgcgggttaa tgctacccac ggcgcgaccg gtaaaccggc actctgccac agaagtttgc
     8461 tgcgtagttt atccattgaa agcgcagatg ccatcactcc gctgccggta taaggcaagc
     8521 ccatcagctc gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga
     8581 taaacacttt ctgaaaaccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga
     8641 caggatacgc gtcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca
     8701 gagaaacttc ccgctcagcg gaggtcccgc ccaacaggac cgcgatttta tcagtcatgt
     8761 tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg agcaattttt
     8821 ccgatattac cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc
     8881 atctcggcta cctgcgccgg gtccggcacc agaatgggat caattttccc acgtccacga
     8941 attgtgcgac acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac
     9001 acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg
     9061 tcgcgcgtac gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag
     9121 cctgcacgcg ccgctttaat agtggcgtcc acttccgtcg ggtggtggcc gtagtcatcg
     9181 accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa
     9241 tcgaaacggc gccccgtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca
     9301 attccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacgacct
     9361 ggcgcattca gggtgacgcg catcggctct ttgtcctggc gcagcagcgt aaagtgcccc
     9421 tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa
     9481 gtcgtggtct gacgccccac acgcggtaac aattcgcgga tcaccggatc atcaacacac
     9541 atcaccgcgc gaccgtaaaa cggcaggttg tgcagaaaat taataaaagt ctgttttaaa
     9601 ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc
     9661 accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac
     9721 cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag
     9781 gttgggtcga gccccgcttc tgcgtagatg ctggaaacca tcgcggtggt tgtcgttttg
     9841 ccgtgcgttc cggcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg
     9901 gcacgacgga tcaccggaat acgcgcttca tgagctgcga caatttccgg gttatcggca
     9961 gaaatcgcgc tggaaacaac gaccacgctg gcatcacgta cgttttccgg gcgatggttg
    10021 aaataaatcg tcgctcccag attcattaac tgctgcgtga ccggatttgg cgctaaatcg
    10081 gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca
    10141 ccaccgatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc
    10201 agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat
    10261 gcaaaaggca tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct
    10321 cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc
    10381 gcgaccaccc ggccagg
//
LOCUS       DAOFSV010000154        10367 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.154, whole genome shotgun sequence.
ACCESSION   DAOFSV010000154 DAOFSV010000000
VERSION     DAOFSV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.154"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(96..593)
                     /locus_tag="QBH27_003972"
     CDS             complement(96..593)
                     /locus_tag="QBH27_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDQ7016230.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(702..935)
                     /gene="yedF"
                     /locus_tag="QBH27_003973"
     CDS             complement(702..935)
                     /gene="yedF"
                     /locus_tag="QBH27_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDQ7016231.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(932..2137)
                     /gene="yedE"
                     /locus_tag="QBH27_003974"
     CDS             complement(932..2137)
                     /gene="yedE"
                     /locus_tag="QBH27_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDQ7016232.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2324..2737
                     /locus_tag="QBH27_003975"
     CDS             2324..2737
                     /locus_tag="QBH27_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ7016233.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(2771..4258)
                     /gene="amyA"
                     /locus_tag="QBH27_003976"
     CDS             complement(2771..4258)
                     /gene="amyA"
                     /locus_tag="QBH27_003976"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDQ7016234.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4336..4701)
                     /gene="fliT"
                     /locus_tag="QBH27_003977"
     CDS             complement(4336..4701)
                     /gene="fliT"
                     /locus_tag="QBH27_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDQ7016235.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKIKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(4701..5111)
                     /gene="fliS"
                     /locus_tag="QBH27_003978"
     CDS             complement(4701..5111)
                     /gene="fliS"
                     /locus_tag="QBH27_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDQ7016236.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5127..6542)
                     /gene="fliD"
                     /locus_tag="QBH27_003979"
     CDS             complement(5127..6542)
                     /gene="fliD"
                     /locus_tag="QBH27_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDQ7016237.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            6790..7839
                     /locus_tag="QBH27_003980"
     CDS             6790..7839
                     /locus_tag="QBH27_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDQ7016238.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            8003..8722
                     /gene="fliA"
                     /locus_tag="QBH27_003981"
     CDS             8003..8722
                     /gene="fliA"
                     /locus_tag="QBH27_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDQ7016239.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            8768..9319
                     /gene="fliZ"
                     /locus_tag="QBH27_003982"
     CDS             8768..9319
                     /gene="fliZ"
                     /locus_tag="QBH27_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDQ7016240.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            9407..10207
                     /gene="tcyJ"
                     /locus_tag="QBH27_003983"
     CDS             9407..10207
                     /gene="tcyJ"
                     /locus_tag="QBH27_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ7016241.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT         2604 a         2609 c         2623 g         2531 t
ORIGIN      
        1 gaatgagaat catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggtt
       61 ggttgagtgg aaaaatgcca ctgaaacgca atggcttaaa gattcgccct cacagccatt
      121 gcaacagtca ctgaaagacc ctgagcgggc ttacaaaaac ttcttccggc ttcgtcacca
      181 cgctcaaacg gtgtgctacc tatcgcggct ataaaaacag cttgtccatc agcacgatag
      241 ataaaataag gctgcttttt gtcgccttct tttttccact caaaccagcc atcggcaaaa
      301 cagattgccc gaccatgttg ccagagcggt ttaaacatgt gactggttgc ggcagtttct
      361 acgcgggcgt taatcagcgg cggtttatcc caccatccgg gagcatatcc ccagaaaacc
      421 ggatccagat gaaggtgttc atcacgttca ctgagcagaa ggactttggt tcccggcgcg
      481 acgttgtatc tgccaatagg ttcgggatcg tagggaatat cgcgttcaat atcttccgcg
      541 agaaacgcga ggtaatcttc acgcgtttgg gattgggcaa agcgtccaca catagaaacc
      601 tcctgagagc acactgaaaa gaataggaga gagggcgggt aaaatctatg tgatagagag
      661 gtaacgatgt tcccgctgtg ggccacagcg ggaatgaggg attacttctg aattaaataa
      721 cgaatggtcg gcccgtcttg ctgaatatcc agcaccgtat agccgtgatt acgcgcatcc
      781 agtggaatat tattgatcga ctgcggacag tcgctcacca cttccaggat ctcccctttt
      841 tttaactgcg gcatcgcctc aagggttgcg actgccggat aagggcaggg ttcacccacc
      901 atatcaagac ggtaatcagg aacgatattt ttcatgcgat ctccttagca gtctgcggtg
      961 ccgcacggcg gaagaagcgt ttttcccagc caataatcaa cattagagca gcaaacaaca
     1021 gcaaatatgt caccagcagg ccgcccatcg ggccaaaggt tttcagcagg ttgattttgt
     1081 cccagtcggt ggccagcgcc ggagcgaaat catcccagta gtacgccaga atcgttgagc
     1141 cgatcacgtt gcccagaccg acccaccagt agtgcacctg gccttctacc gcgcggtaca
     1201 tccagccggt ttcgcagccg cctgccagca cgatgccaaa accaaacagt aaaccaccaa
     1261 ttaccgcgtt tggtcccgcc cacatgattt tgggttcaac gcctaactgt acgtaactga
     1321 agatcccgat ggcacttacc gccataccga taatgattgc tttcgccata tgggtacgtc
     1381 cggtgatcca catatcgcgg aacgccgaag taaagcagat ttgcgcacgt tcaatcagta
     1441 aaccaaagcc gacgccaaac agcattgcca gccccagttt gggctggttc atcgccgtca
     1501 gcagcgccca gcccagcatg ccgaaaaaga ccagcatccc gagacgaaaa cgacgccgcg
     1561 cctgatccgg tttttgcgtc aacggtgagg cagcagaaac tttctgcatt ttcacgggaa
     1621 tacggaagat gggcagaagg gtaaagcgcg cgccaaacca tgaaccaatg gcagtggcga
     1681 tggcaaagaa ccaggcgtgc agcgagaact gtggaattcc ggtaaagaac gccgccaggt
     1741 tacagcccat tgccagacgt gcgccaaaac cggcgataat accgccaatg atggcctgca
     1801 taatgcggat acggctgcgc ggcatgcgca gtttgacatt gttggcccac agcgctgcgg
     1861 caaaacagcc gccaaacata ccgaggatca tcattccgtc gatgcgggtt aatggcgatc
     1921 cttccagatg gataatttta aaataacccc actcttcagc atggacgccg aacaactgca
     1981 ggagctgacc gccccaacgg gtaaattcac ccgtgactgc ccaaaaggtg ccagtgatgc
     2041 caaaatagta agtggagaga atacccgccg cgatgaccgc ggggatgggc gcccagaatt
     2101 taatcaacca ggcgtgtttg aattgctgcc atgacatgaa tgtagcctct gaactcagaa
     2161 ggtttgaaac aagagtcgcg aatcatacac catgcaaatt tattttcggg gtaataattc
     2221 acaaaaataa tggttagatc aaatttctct ctgaacctgg aaaggctggc gacatcaggg
     2281 ctttaatgcc acaatcctct tttcttctca tgcaggacga atgatgaaaa aactcgcaat
     2341 tgcaggtgcg ctcatgttgc tggcgggctg tgccgaagtc gaaaactata acaatgtcgt
     2401 gaaaaccccc gcgccggact ggctgacggg ttactggcag accaaagggc cgcaacgtgc
     2461 gttggtcagt ccggaagcga taggcagcct cattgtcact aaagagggcg ataccctgga
     2521 ttgccgtcag tggcagcggg tgattgccgt gccgggtaag ttgacattga tgtctgatga
     2581 tctcactaac gttaccgtaa aacgtgagct gtacgaggtt gagcgtgacg gtaacaccat
     2641 tgagtacgat ggtatgacga tggagcgtgt ggatcgcccg acggcagagt gtgccgccgc
     2701 gctggataaa gcgccgttac cgacgccact gccgtaatga aatctccccg gcttgccgcc
     2761 ggggatggat ttaaatcacc tcttcgataa cccacacgct gacgctgccg ccgttgcaaa
     2821 agaaggtcgc ttcgccgttt tcgtctgtca ctacgctctc ttgccggttc cccagaaaat
     2881 cgcgccaggt tttattgccg taattctctc ccagatgaat ggttttttcg ccatcatctc
     2941 cgttcgacat gaccaccacg cagccgggat attcgtcggt gccactgcgg ctaaaggcaa
     3001 tgcagttcgg atggtcgaaa aataacgtct gtacaccgtg ggcgaaacgc tgacgggcga
     3061 gaattaactc atcaagctgt tcgattattg gcatatctat cggataggtt tgcccgtcac
     3121 caccgacatc ttcgtaatgt gcaccgtaga ggtccggata gaacaccgaa ggaacgccat
     3181 tttcccgcaa caaaattaag gcatacgcca gcggtttaaa ccacggttcg acgggggctt
     3241 cgagggcttg caacggttgg gtgtcgtggt tggcaacgag ggttacggcg tgaaaaggat
     3301 cggcttccac cagcgtccca gtgaagatct gcgtcatgtc gtagtcgcga cccatgcgcg
     3361 atgcttcatg gaatttcatc tgcagcggcg catcaaacag catggttttg ccttccacct
     3421 gatcaatata cgtttgcagt ttatcgactt catgcgacca gtattcagcc acaataaaca
     3481 acggctttgg cgcaacttcc tgtacgtgtt cgatccactc tttataaaac catgccggaa
     3541 tatgtttgac cgcatcaaga cgaaagccgt cgcattgcgt ttgttccatc acccagcgcg
     3601 cccagtattt aatctcttcc gtcacggcat gattgcgaaa atcgatattc tcccccatca
     3661 gataatcgaa attaccgagt tcatcatcaa cctgatcgtt ccagccttct ccggtatagt
     3721 cgttaacaat tttaaaaatg ccatcttcat cagggttttc gatatggtcg ataccgctaa
     3781 aacatttgaa atcccagata aactgcgagt attgcccggc acgagcgggg aaggtgtaac
     3841 gcgtccagcc ttcacactca atgatttctt cgtcaatttg cgtacggtca tcagcattta
     3901 cacgctgcac gcgaatagct tctttttcat ccgcgcccat tttgtggttg actaccacgt
     3961 ccagcagcac cgcaatgtca ttacgtttca gggcgtcaat ggcggccagc agttgtgctt
     4021 tatcaccata tttagtaggg atgctgcctt tctgatcaaa ctcacctaga tcaaataaat
     4081 cataggagtc gtagccgacc gaatagccgc ccgatgcgcc tttataggcg ggcggcaacc
     4141 agaccatatt gataccaata tcattaaaac cgtctgcgcg ctcggccagt tcaggccaga
     4201 gcttaccgcc ttccgggtaa taccagtgaa aacattgtaa cagcgtggga ttacgcattc
     4261 ttcgactcca ttcaagggga atattagaag cgtagccgta atcggattat tcgcgagcca
     4321 tcggctcatt aagattcaaa agaggttatc ctgcggagcc agcacaaagc cgccctgatc
     4381 gccataggca cttaacaccg atttttgcac cgatgactga ccgaccagtt tcgccagttc
     4441 atccatccga atctgtaata actgctttat cttgctttcg ttgtcgagaa tcaggcgcag
     4501 catcgggcgg agctgctcct gcatcgtagt ggacggggca acctcttcag tcaggtgtgc
     4561 gatctcctgc accgcattca catacgccat ttcgctggcg atgagttcgt cccattgttc
     4621 ttccgttgcc aggcgtaaca tgagctggct tttttcgacg agttgttgcc aggcgaaata
     4681 caaatgcggt gcattgttca tcagactggg tcctgaatca aagaagggga gagtaaagac
     4741 tctttccagg catcggcaat attgcgcatt aatgcttcca cttcttcgac tgcggagaca
     4801 tcgttgcgta aattggcttg cagcaaacgc ctgaccatat agctataaag agcaatcaag
     4861 ttttgggtta gttcgtcttt gctctcttca tcaagactca cccgcagtcc gttctcaatg
     4921 atgttgatcg cttttgacaa agagacgcct ttgccttgct gattgttgtc ctgcataaac
     4981 aggcgcgctc taaccagtgc gctcaacact ccatcaaata gcatggtgac caactgctgt
     5041 tggctggcgc tcattacggc gctttcgacg ccaatttgtg cataggcctg ggtgcctttt
     5101 gccgcgtaca tagtctctcc tgaagattat ttcgagttgc tatttgaggt gtcgaactgc
     5161 tgtgtcagat agctactagt ggagttcagc gagttcatta acacatctaa ttgggtgaac
     5221 tgggctttat agcgcgtcat taaggtatcg atacgttcac tggctgcgtt gtactggtcg
     5281 gtcaaattat tcagcgtttt gctgacgcca tcttttgctg cctggattat cccggtggaa
     5341 gagagccaac tggtcaggtt gctactaatg ccggtggtta ccccagtact tttgccgtca
     5401 cctataaaca tttcaccgat accagcggca tcatttttta gcgccgtttg cagtttgtcg
     5461 gtcgcgattt ccagcttgcc ggtactggca tcgctggtaa tgccaatctg tgccagcgtt
     5521 ttatagtttg aactgctgtg agtattggcg agcaaggttt taagctgagt ctggatggta
     5581 cgcagtgtgg agtcaccgag taatgcgcca ttactggagt cctgactttc agctccggca
     5641 tcgacagcgg tgtatttggt taagctgctg aatgtatcct gcaatgtgtt ataggcatcc
     5701 acccacgctt taaccgcatt ttcagctttg gatgtgtctt tactgatggt cagtgtctga
     5761 ttaccggtgg tgacgtcatt caggttgagg gtgatatctt ccagtgagtc gctgatagtg
     5821 ttactgctgt tttcaatctc aacgttattg actgtcagtt tggcgttttg cgcggtgacg
     5881 ctttcaatca tgccattgct gctatcaccc ggcgttccgt tgtaacccat aaaactttcc
     5941 agggcgctgt cgccgctgac gctaagtttc atagcgttat tgctgcccgt atcgttagcg
     6001 gtgatggaca gacgatattc gccgtttccc acattgatga tactggcgct gacaccagct
     6061 ttggcgttgt taatggcatc gcgaatgccg gaaagtgacg agttacccgc gctgatatca
     6121 atcgtgaccg gatctttacc gccgccttgt tggatggtca gtacgctatc gctggtggcg
     6181 atggcggctt tactgtcttt ttgactattt tttgttgtca gcgtctgtgc ctgcgccagt
     6241 tggctaacgc taatggtgta tttcccagca atcgcgctgc ccgtggtggt ggcgctgaaa
     6301 gccgatgaac tgctggtggt tgaggtggcg gtaaataaat cggctttatt cagtgctgtg
     6361 ttggctgtct ggaaactctc tagtgcactt ttcagcgtcc cgtaggcgct cagtttcgcg
     6421 gtgtaagagc tttgctgttt tgatatcggc gtcagggtag atttttctgc cgcctccaga
     6481 ctgtcaagga tgctgcttaa gtccagcccc gacccaaccc caagcgttga aatactagcc
     6541 atatagtttc cttaatcatg cgacgtgtat agaacgaata ccggggttat cggcgtaagc
     6601 ggggtaaagt ttacgattta ttttttgcct taatggcacg aacagcaacg aggaagggga
     6661 gtatttcgac cgctagaaaa aaattctaaa ggttgtgagt gaccagacga taacagggtt
     6721 gacggcgacg aagccgaagg gtggaagccc aatacttaaa ccgtagactt gaaaacagga
     6781 aaatgaatta tggcacaagt cattaatacc aacagcctct cgctgatcac tcagaacaac
     6841 atcaacaaaa accagtctgc gctgtcgact tctatcgagc gcctctcttc tggtctgcgt
     6901 attaacagcg ctaaagatga cgccgcgggc caggcgattg ctaaccgctt tacttctaac
     6961 atcaaaggtc tgactcaggc cgcacgtaac gccaacgacg gtatttctct ggcgcagacg
     7021 gctgaaggcg cgctgtcaga gattaacaac aacttgcagc gtattcgtga actgaccgtt
     7081 caggcctcta ccggcacgaa ctctgattcc gacctgtctt ctattcagga cgaaatcaaa
     7141 tcccgtcttg atgaaattga ccgtgtatct ggtcagaccc agttcaacgg tgtgaacgtg
     7201 ctgtcgaaaa acgattcgat gaagattcag attggtgcca atgataacca gacgatcagc
     7261 attggcttgc aacaaatcga cagtaccact ttgaatctga aaggatttac cgtgtccggc
     7321 atggcggatt tcagcgcggc gaaactgacg gctgctgatg gtacagcaat tgctgctgcg
     7381 gatgtcaagg atgctggggg taaacaagtc aatttactgt cttacactga caccgcgtct
     7441 aacagtacta aatatgcggt cgttgattct gcaaccggta aatacatgga agccactgta
     7501 gtcattaccg gtacggcggc ggcggtaact gttggtgcag cggaagtggc gggagccgct
     7561 acagccgatc cgttaaaagc actggatgcc gcaatcgcta aagtcgacaa attccgctcc
     7621 tccctcggtg ccgttcaaaa ccgtctggat tctgcggtca ccaacctgaa caacaccacc
     7681 accaacctgt ctgaagcgca gtcccgtatt caggacgccg actatgcgac cgaagtgtcc
     7741 aacatgtcga aagcgcagat tatccagcag gcgggcaact ccgtgctgtc taaagccaac
     7801 caggtaccgc agcaagttct gtctctgtta caaggctaat ggccttaacc tgcctgaccc
     7861 cgccaacggc ggggtttttt ctgcccgcaa tttaccggta acccccaaat aacccctcat
     7921 ttcacccact aatcgtccga ttaaaaaccc tgcagaaacg gataatcatg ccgataactc
     7981 atataacgca gggctgttta tcgtgaattc actctatacc gctgaaggtg taatggataa
     8041 acactcgctg tggcagcgtt atgtcccgct ggtgcgtcac gaagcattgc gcctgcaggt
     8101 tcgactgccc gcgagcgtgg aacttgacga tctgctacag gcgggcggca ttgggttact
     8161 taatgccgtc gaacgctatg acgccctaca aggaacggca tttacaactt acgcagtgca
     8221 gcgtatccgt ggcgctatgc tggatgaact tcgcagccgt gactgggtgc cgcgcagcgt
     8281 gcgacgcaac gcgcgtgaag tggcacaggc aatagggcaa ctggagcagg aacttggccg
     8341 caacgccacg gaaactgagg tagcggaacg tttagggatc gatattgccg attatcgcca
     8401 aatgttgctc gacaccaata acagccagct cttctcctac gatgagtggc gcgaagagca
     8461 cggcgatagc atcgaactgg ttactgatga tcatcagcga gaaaacccgc tacaacaact
     8521 gctggacaat aatctgcgcc agcgggtgat ggaagccatc gaaacgttgc cggagcgcga
     8581 aaaactggta ttaaccctct attatcagga agagctgaat ctcaaagaga ttggtgcggt
     8641 gctggaggtc ggggaatcgc gggtcagtca gttacacagc caggctatta aacggttacg
     8701 cactaaactg ggtaagttat aacgtcagta aatgccgcac tttaattttg actaccagga
     8761 gttcttaatg atggtgcagc acctgaaaag acggccatta agccgctatc ttaaagactt
     8821 taaacacagc cagacccatt gcgcgcattg ccgtaaatta ctcgatcgca ttaccctcgt
     8881 tcgcgacggc aaaatagtga ataaaatcga gatttcccgc ctggacacgc tgcttgatga
     8941 aaatggctgg caagtggaac aacaatcatg ggcggcattg tgccgatttt gcggtgattt
     9001 acattgcaaa acgcagagtg attttttcga tattatcggc tttaagcaat ttctttttga
     9061 gcaaactgaa atgagccctg gtacggtgcg tgaatatgtc gttcgtctgc gccgtttggg
     9121 gaatcatcta cacgagcaaa atatttccct cgatcagctg caggacggtt tcctcgatga
     9181 aatcctcgcc ccgtggctgc cctccaccag caccaacaat taccgcatcg cgttacggaa
     9241 gtatcaacac tatcagcgtc aaacgtgtac cggacttgtg cagaaatcct ccagcctgcc
     9301 tgcttctgat atatattaaa taagaataag atgtagcgga gttgtttttg tgtttacaaa
     9361 caatggctct acactgcaaa cagacataac aacattcggg gtgaatatga aattagcaca
     9421 tctgggacgt caggcattga tgggtgtgat ggccgtggcg ctggttgcgg gtatgagcgt
     9481 taaaagtttt gcagatgaag gtctgcttaa taaagtcaaa gagcgcggca cgctgctggt
     9541 agggctggaa ggaacttatc cgccgttcag ttttcagggg gatgacggca aattaaccgg
     9601 ttttgaagtg gaatttgccc aacaactggc aaaacatctt ggcgttgagg cgtcactaaa
     9661 accgaccaaa tgggacggta tgctggcgtc gctggactct aaacgcattg atgtggtgat
     9721 taaccaggtc accatttctg acgagcgcaa gaaaaaatac gatttctcta ccccgtacac
     9781 catttctggt attcaggcgc tggtgaaaaa aggcaacgaa ggcaccatta aaacggctga
     9841 tgatctgaaa ggcaaaaaag tgggtgtcgg tctgggcacc aactatgaag agtggctgcg
     9901 tcagaatgtt cagggcgtcg atgtgcgtac ctatgatgat gacccgacca aatatcagga
     9961 tctgcgcgta ggccgtatcg atgcgatcct cgttgatcgt ctggcggcgc tggatctggt
    10021 gaagaaaacc aacgatacgc tggcagtaac cggtgaagca ttctctcgtc aggagtctgg
    10081 cgtggcgctg cgtaaaggaa atgaagacct gctgaaagcg gtgaatgatg caattgcgga
    10141 aatgcaaaaa gatggcactt tgcaagccct ttccgaaaaa tggtttggtg ctgatgtgac
    10201 caaataatca gcataatgac aaaaaagggc gctttcacta gcgccttttt tatttacgcg
    10261 ttttcagcgt gcataataag aaaatcacaa caacacacat cggaggctgt tatgccactg
    10321 cataatttaa cccgttttcc acggctggag tttattggcg cgccaac
//
LOCUS       DAOFSV010000155        10341 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.155, whole genome shotgun sequence.
ACCESSION   DAOFSV010000155 DAOFSV010000000
VERSION     DAOFSV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.155"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..358)
                     /gene="yciA"
                     /locus_tag="QBH27_003984"
     CDS             complement(<1..358)
                     /gene="yciA"
                     /locus_tag="QBH27_003984"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDQ7016242.1"
                     /translation="MSTAHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVA"
     gene            complement(463..1002)
                     /locus_tag="QBH27_003985"
     CDS             complement(463..1002)
                     /locus_tag="QBH27_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDQ7016243.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1032..1775)
                     /locus_tag="QBH27_003986"
     CDS             complement(1032..1775)
                     /locus_tag="QBH27_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDQ7016244.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(1803..1892)
                     /locus_tag="QBH27_003987"
                     /pseudo
     CDS             complement(1803..1892)
                     /locus_tag="QBH27_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2131..2769
                     /gene="ompW"
                     /locus_tag="QBH27_003988"
     CDS             2131..2769
                     /gene="ompW"
                     /locus_tag="QBH27_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDQ7016245.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            complement(2815..3945)
                     /locus_tag="QBH27_003989"
     CDS             complement(2815..3945)
                     /locus_tag="QBH27_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HDQ7016246.1"
                     /translation="MARPRKYKTNVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(3923..4171)
                     /locus_tag="QBH27_003990"
     CDS             complement(3923..4171)
                     /locus_tag="QBH27_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDQ7016247.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            complement(4236..6707)
                     /locus_tag="QBH27_003991"
     CDS             complement(4236..6707)
                     /locus_tag="QBH27_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDQ7016248.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGSQQNTDASDTTAQ
                     PDLVKVTADEVNKIMQAASISQPDANKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     HQNQSETEQYDQKAEQNSQNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCG
                     AVMGDATYQETFNEESQDEAREKDPEEIENAGLPNKECTEGDQHANDNNEIGETANPL
                     IKVNSHRKSTSTSRLWHHLMIDLETMGKNPDAPIISIDAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFENTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPNQADF"
     gene            complement(6800..6991)
                     /locus_tag="QBH27_003992"
     CDS             complement(6800..6991)
                     /locus_tag="QBH27_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDQ7016249.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(6988..7176)
                     /locus_tag="QBH27_003993"
     CDS             complement(6988..7176)
                     /locus_tag="QBH27_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDQ7016250.1"
                     /translation="METLLPNVNTSEGCFEIGVNISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            complement(7742..7960)
                     /locus_tag="QBH27_003994"
     CDS             complement(7742..7960)
                     /locus_tag="QBH27_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016251.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARTKQAE
                     NLSALEQDITRLEKAAALSIARMVTYPR"
     gene            complement(8120..8275)
                     /locus_tag="QBH27_003995"
     CDS             complement(8120..8275)
                     /locus_tag="QBH27_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDQ7016252.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(8548..9264)
                     /locus_tag="QBH27_003996"
     CDS             complement(8548..9264)
                     /locus_tag="QBH27_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7016253.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            9314..9529
                     /locus_tag="QBH27_003997"
     CDS             9314..9529
                     /locus_tag="QBH27_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ7016254.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            9526..9951
                     /locus_tag="QBH27_003998"
     CDS             9526..9951
                     /locus_tag="QBH27_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDQ7016255.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            9974..>10341
                     /locus_tag="QBH27_003999"
     CDS             9974..>10341
                     /locus_tag="QBH27_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7016256.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDP"
BASE COUNT         2566 a         2467 c         2435 g         2873 t
ORIGIN      
        1 ccgcgacata cttaaataat gcttctgttg ctttatagcg ttgcccgatt ggttctgacg
       61 ccactttttt cacccacact tcaatattaa tgctgactga tgtcgtccct ttctggacac
      121 agcgtgcata gcagcacacc acatcgccga ccgcaaccgg ccgtaagaaa gtcattcctt
      181 caacccgcac ggtcactaca cgaccgtggg caatttcttt cgccagaata gcgccgccaa
      241 tatccatttg tgacattaac caaccaccaa agatgtcacc attggcattg gtatcggcgg
      301 gcatggctaa agtacgtaaa acaagatcgc cctgagggac gttatgtgct gtagacatgg
      361 taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac tatgattttc ctgaggagaa
      421 cagaggagat acagcgccag ccccgaagga ctggcagtct ggttaggatt tatcttcctg
      481 cggcatgtgg cggtagatat agataccgct taacaatgtg aagattaagg taagggcggt
      541 caggccaaag actttaaagt tgacccaaat attttgcggc agccagaatg cgatgtagat
      601 gtttgccagg ccgcaaagga taaagaaaac agcccaggcc agattcagct tcgaccatac
      661 cgattgcggc agggtgagtt ctttacccag catccgctga attagcggct ttttcatcac
      721 ccattgactg actaacaggg cacccgcaaa cagggcataa atgacagtaa ccttccattt
      781 aataaactca tcattgtgaa agaacagcgt caagccaccg aagacgacca ccagaacaaa
      841 agtgatcagg gccatcttct caaccttacg aaagcgaacc cagctataga taagcacaat
      901 cgccgtggcg acgatcagcg ccgcagtagc cgcatagatg tcataaatct tgtaaaacgc
      961 gaaaaagaca accagcggta aaaaatcaag aaactgcttc attttatgat tccgtaatca
     1021 aatgcttaag gttattggcg aatcaacata tacaggcgga acagatagat gagcaatacg
     1081 gctgaaatca ggttgctcaa ggtgttcgcc agtacggcac caatttccgg ggttaatgcg
     1141 gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca gcaaccagct cagtactgcg
     1201 ggtgccacca gacgcatatt cgcccatgtc agccgcatac tgctacgcat cgaggcaaaa
     1261 acgcccattt tgtcctgtac cagcatcacc ggtgccagag ccagtaaaat ggccatgata
     1321 attcccggaa cgaccaccag catgatgcca atctgtacta aaagggtagt cagaaaaatc
     1381 agaataaata actttggcaa tatcggcgca ctggcaccaa tagctcgcag cgcactgact
     1441 ctctgacccg cagacaccag ctggataatt aatattacgc ccccggcgag aatggcgtta
     1501 ccgattaatc ctgaaaaagt ggacgccgct gaagcctgca gcaaaatttg ttgctgttcc
     1561 ggtgacatat tctgaaccag gtcgaacaac ccactactgc cgctaacggg tacgccgtca
     1621 ttgagctgcg caagctgtgc atcactgggt gagaaaacat gccctaacac cactgtgata
     1681 aacgcacata gcaacgatac caacagaatg gtcataaatt gattacggaa gaaatttccg
     1741 gtgtcacggt atacggactg cgccgtgata gacatgcact ctccttgagt tttatgcagg
     1801 tgttaattag cgggcaattg taccctggtt aagccccggg tggcagcatc aggtcttgta
     1861 tggaaaagca tatctttgta aagcggaggt aattgctggc cttcaggcgt gggtaaaccg
     1921 ctgcaagtgg cgagcgagtt aatatttgcg tagcgaaata tataaaaatt gatttaaatc
     1981 acattaacca ggattttcaa tgcaacttct aaattaatct agatcaataa agggtgaatt
     2041 accatatgta atgtgatctg tgtaggatca tttgttaccc caatgtgggt atattcgtca
     2101 cgtttttata accataacga cggagcggat atgaaaaagt taacagtggc ggctttggca
     2161 gtaacaactc ttctctctgg cagtgccttt gcgcatgaag caggcgaatt ttttatgcgt
     2221 gctggttctg caaccgtacg tccaacagaa ggtgctggtg gtacgttagg aagtttgggt
     2281 ggattcagcg tgaccaataa cactcaactg ggcctgacgt ttacttatat ggcgaccgac
     2341 aacattggtg tggaattact ggcggcatcg ccgtttcgtc ataaagttgg cacagccaca
     2401 accggggata ttgcgacagt ccatcattta ccgccaacac tgatggcaca atggtatttt
     2461 ggcgattctg gcagtaaatt ccgtccttac attggtgcgg gtattaacta caccaccttt
     2521 tttaataatg acttcaatga caccggtgaa ggtcttggtt tatctgacct tagtctgaag
     2581 gattcatggg gcgtggcggg gcaagtaggc ctggattatc tcatcaaccg tgactggttg
     2641 ctgaatatgt cagtctggta catggatatc gataccacgg ctaagtacaa atctggtgtt
     2701 actaccgtaa aagactcggt acgactggat ccgtgggtgt ttatgttctc cgcaggatat
     2761 cgtttttaat tttctctgca aaacctctgc aaaacccctc tgcaaaactg gtcatcaaat
     2821 gaccagtttt ttccattcct taccgcgtgc gtcgttgtaa atatcggtca ttttttgatt
     2881 cgaatggccc agcaaaattt tggtatcaac cccctgctct ctgaacaatc gctctgataa
     2941 agatctctgc tcatggaaag agggtggggt gccattagca cgccagttgt aatccacaga
     3001 atcccgggct tttttaaatg caacggttaa tgttgctggc ttaaccatcc cgccgcgctt
     3061 agctgtccct ttcgcgtgat ggtggtgcaa tagccacgga ctaagaacgc aatcgcggca
     3121 ggatgacacc acatcatcta gggtgagatt taatttatcg caacgcagag ccagagggat
     3181 ggcaatccgg gttcctgttt tttgctgttc gacatgaaga taaccatccc ggatatccga
     3241 aaattgcatt ttgcaaatat ctgaaaggcg ctggcctgtc atcagtgcca gcagcatacc
     3301 gcgctgtaaa aagtaaccat ccttttccgc tgcgttataa atcatcatcc actcatcaaa
     3361 agtcagtcgc tgtcttgata tccgcacctg cggttttttt gccgattctg cagggttaaa
     3421 gcctggcggg acatcgcccg tttgctgagc ttcccggaaa acatcgatca gtaccttcct
     3481 gaaaatttgt cccattctgt tatgtcctct ggccttgtac tcttccagta ctgataccac
     3541 atcttttacg gttatggcat ctaacggtct ggtgccaaaa cgttcatcaa ataccctgag
     3601 aggggccgct ttctgtttca gcgtgttgag tttgatctcg ccgttttcat atctttcctg
     3661 ttgaattttt ctgtaattat tcagaaaaat ggtaacggtt gatgaaccgc cggtatcact
     3721 aataattttc tcctgcagac tgagcatttg ttccatttgc tgccgggcaa gacggctgtt
     3781 cgcttctgct gcaatagttt ctgccagttt ctggtcaata ctgccgagac cgtgattttt
     3841 gcctgttatg ggatgcctgt aacgccagta aactttgtta tttcttttgt caaaatacgg
     3901 agataatccc ggaacattgg ttttatattt tcgcgggcgc gccatcttcc agtatcctct
     3961 tcaaagcagg gtgatctgtg gcgatcacct ccggcttgtt taccattccg acaaagcgag
     4021 cttgcggatc cactcgccag cgtcttccaa cttttttggg gagaggaaat atcattccgg
     4081 ctttagcgta tttacttaac gtactcggag ttgggaccgg ttcactgaat tcctcttttg
     4141 cccactcagt gagcagaata agtcttgcca tgagcgtcgt tcgctaatca tggtcgccgc
     4201 cactatagct ggtgggcaac gaccggggtt gaacattaaa aatcagcctg attcgggatc
     4261 agtttttgcc agataactga aacgtatttt gcctggtaac gggcgtcatc aagtgcatta
     4321 tggcgctcac cttcgaatgg aatagccgtt ctggcatcga agtctatggc tttccccagc
     4381 tcaacgattg tgcgtacatc gcgatcgttg tagtaacgcc atgggcaggg gatcccctgc
     4441 cgttcgtatg aacggcgcaa aatcgtgttt tcgaagttgg ctccatttcc ccagacctga
     4501 acaaaaaatt caccggagtt ttcgtcgata aattcccgca attgtaacag tgcatcatct
     4561 aacgggattt catcggtcat aatggcagac tgcgcttcac gtgattgctt cagccacctt
     4621 ttaatgacgt cacgatcaat gactccgcca gcagtttcca gatcgatagt cttactaaat
     4681 tccggtccca tatctccggt ttgcggatcg aaaaatattg catctattga gataatcggg
     4741 gcatcaggat tttttcccat ggtttcaagg tcaatcatca gatggtgcca caacctgctg
     4801 gtggatgtgc ttttacgatg actgttcacc ttaattaagg gatttgctgt ctcgcctatt
     4861 tcattattgt cattggcatg ttgatcgcct tcggtgcact ccttgttcgg gagtccggca
     4921 ttttctattt cctctggatc tttttcccgg gcttcatcct ggctttcttc gttgaatgtc
     4981 tcctggtagg ttgcgtcacc catcactgcg ccacaatcag ggcagttacc accaccagtc
     5041 tgattgcatg cggtacaaac tttttcctct tcctgttgca cgactggttc aggttgtttc
     5101 gtttctggct cgttttgtaa cgcattttgg ctgttttgtt ccgctttttg gtcgtactgt
     5161 tccgtttctg actggttctg gtgcacagaa tcgcgggttt caatcccctt cacccatttc
     5221 ggatcattcg ggtcgctaat cccttcaaca aattcaccac gtgatgcagc gagcaactta
     5281 ttagcgtcag gctggctgat actggctgcc tgcataattt tgtttacttc gtcagcggta
     5341 acttttacca ggtctggttg tgcggtcgtg tcagatgcat cagtattttg ttgtgaacct
     5401 gtgtatgtgc cgtttttgcg ggcaaaatat tcttctttcg tgatttcagt tgccccggca
     5461 gccagcgcct tatccagacc agaaagtttg tttgcgcgac cgtatttttc gccatccttg
     5521 tcggtgaaga ggaagtagaa cggcccctca cgctctacag atggttcgac ttccactttg
     5581 cattcggttt tttcgttgtc cggaattgcc gtttccactg catcagtttc tggtactggc
     5641 gacgagagag tatcagttgc gctctgattt cttccttcat cttcaaacac gccctttgta
     5701 gtcaggtatt cagtaatgta tttgttcagt gccacagggt ctttgtgaat gtcgatcgga
     5761 cgttcacgga caaggccaaa aatagtctgg cggtcgtagc gaagggcatc aggctgtttg
     5821 cgcattgatg ccgagatacg cttccagtct tcgcggtcgt tgtcgataac ttcatttttt
     5881 gcccagcgat ggatgctgcc gtcaatgttt ccggcatcca catcaccagg ccagagagcg
     5941 taggccagtt cgtcatccag tgttttccat gtctgcttgt attcgcgatg aatggcagca
     6001 atgaccgggc tgatttttcc tgttgaattt tcagtgtact gttgattggc tctggcgcgg
     6061 gcgagatcaa caacagacgt gtattttccg gtttccttgc gttcaccttc acgacgtttt
     6121 ttccagatgc gcatctctgc ctgaatttcg ggccatttgg caccaggctt acatttatgc
     6181 ttaacccacc cgatggcatg cagcttaagc tccggataca tggcgttaac ttctggcatt
     6241 ttcatcaacg cttcaacgat atgtccgtcg aatgttgcca tgtcttcctg caacaattcc
     6301 tgtgcgctaa taaccatatc aacggtgatg ttttcacatg tgtcgaactt aaccatgaca
     6361 gcgttctgta cttcaggggc cagcttgtca aaagtgacgt tcatcggatc ggattcagtc
     6421 tcaaccggga caaaggaagc agactcctca tcccagcggt tttcctgcat atattcagca
     6481 tcccaggaat cgagggcagg gcggggtata ccgggtttat cctcgcagac aagaaattta
     6541 taagcgcagt cctgagcagc cggataatgt tccaggaatt gccagtgaaa ttttgcgcgg
     6601 gcgcgacgtt catcaccggc ttcaatggca gtggctacag cgacggcacc ttcttccttt
     6661 attgcctgtt cgtccggaat ggcggcgcaa ataaagactt tactcatttt gttttacctc
     6721 attacagatt taagggtgaa caaatccctg ccattgctgg catataagaa tgaaaccgga
     6781 tatttattac ggaactgttt taaagacctg ccgggatttc gttattatcc tggtgaataa
     6841 ctttatcgac cgggtaacag ttaccgggaa ttttctgttc ggttgctgca gtcacacact
     6901 cctgcattgt cctgtgaaca ctgactgcaa tatcaactgg ctctccggaa acaagaaaaa
     6961 ctgtcagaac aagtgcaaat gctgtattca ttgccagcat cctttttgta tcggacgtaa
     7021 acgggccagc attgaaagaa tgcatatttt atttaatagc tcccgttcgt gttttctctt
     7081 gttaatggca tcttcagtaa atacagggtt actgatattg acaccaattt caaaacaacc
     7141 ttcagacgta ttaacgtttg gtaataacgt ttccattatc gcgtcctcaa caatgaattt
     7201 tgtgatgcgg tgcctggtgc ctccaggtga cgttaaccag ttaacaatta acgccggata
     7261 cagagaatcc acccataaca ctgtttttgg ttttaactgt tccgcgtgcg ctcagccgca
     7321 ttcaccacat cacaaaattc actttaaaaa gggcggcaga gcagtcacgg agtaaaactg
     7381 ataccgccaa acgtcaccag aaaattgata acagagggcg ttgcagcgga gttgtcactt
     7441 aagcgtatgg tcaacctgac aacccggtgt cctcaacgga ggaaggaata accccgccat
     7501 acttaccgcc gcgccatttc gcggagtgcc acaaccggaa gcgcacggtc gaactaaatt
     7561 taacgacacc gtacagagag accaatttcg ccgtgcgctt tcgctttatg ccctgacttt
     7621 tcagggacat atcctttcag taaactgtca gtgccggatg ttcacccgtg tccggcgcac
     7681 gcactccacc tgacccgtgg agaactcctt aattaccaac cttagcttcg ttggttagct
     7741 attaacgcgg gtatgtaacc attctggcaa tgcttaatgc cgctgctttt tccagcctgg
     7801 tgatatcctg ctccagagcg gacagatttt cagcctgctt agtcctggct tcattggccc
     7861 atttcagatc ctgcgctgca ttaattttct ggcgcatcca ctcataaagt tcatcatcgg
     7921 tatagtctgg cgcgatgatg acgggttctc gtttctgcat actgattcct cgcggtgctg
     7981 cttcgcttat cagccgttag attttgccga gctggaaagc gcctgtttaa actcactgaa
     8041 gctgagagct tcttcgcctt cggcaaggcc ttcgaagtat tcttcgtaag ccttttccat
     8101 gattgtgtcg aaatccatat cactcacctg agtttctttc cagccagcga cgggcaccat
     8161 tttcggtttt aaacgttttg cttttggtat acgtcattgc ggtgaacgtg ccgtcctggt
     8221 tggggaacac gccacatacc agagattcgc tgttgccaag atcgatagta tccatgctga
     8281 cctcatttcc ccttaacgcc gggggagcgg aacaaaaacc tgctgcatag ttattaaagt
     8341 tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac ttaccccctg accactgctt
     8401 ggtaactcga agtattgccc ggcgttctgt ggggcggggt gggttggtat gttgttaagg
     8461 taacaagagt tacctttcga gtcaatacaa tgttgcaaaa ggtacatttg agggcataaa
     8521 aaacccgcaa tgaatgcggg ttctgactca gtctaagtat tgatgtattt gtgaaacttt
     8581 acctttaatg gtgtaaccac cattcagttc gatgggtttg taaagcggat tcagtgacaa
     8641 cagatagatg tttggtccgt caatcgcaac tttttttagt gttacgtttg gcgttccttc
     8701 caattggatt aagattattt ttcccaccag ttctctaatg ttacttgagc atggtgtgat
     8761 cagcacggta gatccgtcgg ggatggttgg gaggccgtta gagtttgtca tcgcatctcc
     8821 ctcaacatgc aataaaaaag agttttcagc ggtttttgtc atgacatcaa cccagttctt
     8881 aataccagga atcttggtta ctggacaact catatcccaa taaccagcct gttcccacgt
     8941 taaaacgggc aaccgggcga tgttgtcact aatgtaaggg tactgattca gacgcagatc
     9001 atcggtttta tcgtgaccgt cctttccata aagaatccat tcaggagatt tggaaagcaa
     9061 ttttgacagt agatacaaat tctcaccgtc aggttttgaa gagccatttt cccattttgt
     9121 tacggataca cgagatatgc cgattgcttt cgcaacctgc tgttgggtta atccaacgtc
     9181 ttttcgacga ttccgaatac gttcgctgat agtgtttttc atgtaaccaa tgttactacc
     9241 aagtgatgtt gctatggttg acattgttat gtaactattg ttacccttct gctcgaaata
     9301 acaggagagt tttatgttca aagatgatgt tctgcgctat ttcaaaaaaa agcgactagt
     9361 agctgaggct cttggaattt cacatgtggc tgttgtgcgg tggaaagcag ttattcccaa
     9421 acttcgcgca atggaactgg atgaaattac taacggtgaa ttgaaataca acccagaact
     9481 ttacaagaag caggatagca cctcgaacga aggaaagaat gattcatgaa aatcaagcat
     9541 gaacacatcc gcatggcgat gaatgcctgg gcgcatccgg acggcgaaaa agtgccggct
     9601 gcgaaaatta ccaaagcgta tttcgagctg ggaatgacgt tcccggaact gtatgacgac
     9661 agccatccgg aagccctggc ccgtaatacc cagaaaattt tccgttggct ggataaagac
     9721 acccctgatg ctgttgaaaa aatgcaggct ctgttaccgg cgatcgaaaa ggcgatgccg
     9781 cctttgctgg tggcccgtat gcgcagccac agttctgaat attaccgtga gatcgtcgaa
     9841 cggcgggatc ggctggtgaa ggatgtcgat gattttgttg cgtcagcggt tgttttgtat
     9901 gaccagatga atcgcggcgg cccggcaggg aatgctgtgg tgatgcacta aaagcacggt
     9961 gttcgggggt tttatgagca gcaagcttca tggtcttgtc tgggaagggt gcgccttcac
    10021 cggcatgatc ttatccaggg tggcggttat ggcccgtctt gcagactaca gcaatgacga
    10081 gggcgtgtca tggcctgcca ttgaaactat ccggcgtcag atcggtgcaa gaagtgaatc
    10141 cacagtgaaa tcggctattg cagaactggc gaaagagggc tggctgacga aggaagagcg
    10201 taaggtcggt gggcgtaatg taagcaatat ctatcggctt aatgtggaaa aactcgaagc
    10261 agctgcggcg gcggcgcgtg agtcatataa accgaaaaga aaaattagcc cggcaaaaaa
    10321 tgacccgtta acagttgacc c
//
LOCUS       DAOFSV010000156        10254 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.156, whole genome shotgun sequence.
ACCESSION   DAOFSV010000156 DAOFSV010000000
VERSION     DAOFSV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.156"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..288)
                     /gene="ygaZ"
                     /locus_tag="QBH27_004000"
     CDS             complement(<1..288)
                     /gene="ygaZ"
                     /locus_tag="QBH27_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDQ7016257.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPS"
     gene            complement(412..1596)
                     /locus_tag="QBH27_004001"
     CDS             complement(412..1596)
                     /locus_tag="QBH27_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016258.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(1788..2780)
                     /gene="proX"
                     /locus_tag="QBH27_004002"
     CDS             complement(1788..2780)
                     /gene="proX"
                     /locus_tag="QBH27_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDQ7016259.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNANTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(2837..3901)
                     /gene="proW"
                     /locus_tag="QBH27_004003"
     CDS             complement(2837..3901)
                     /gene="proW"
                     /locus_tag="QBH27_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDQ7016260.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(3894..5096)
                     /gene="proV"
                     /locus_tag="QBH27_004004"
     CDS             complement(3894..5096)
                     /gene="proV"
                     /locus_tag="QBH27_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDQ7016261.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDVELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(5452..6411)
                     /gene="nrdF"
                     /locus_tag="QBH27_004005"
     CDS             complement(5452..6411)
                     /gene="nrdF"
                     /locus_tag="QBH27_004005"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDQ7016262.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(6421..8565)
                     /gene="nrdE"
                     /locus_tag="QBH27_004006"
     CDS             complement(6421..8565)
                     /gene="nrdE"
                     /locus_tag="QBH27_004006"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDQ7016263.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(8538..8948)
                     /gene="nrdI"
                     /locus_tag="QBH27_004007"
     CDS             complement(8538..8948)
                     /gene="nrdI"
                     /locus_tag="QBH27_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDQ7016264.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(8945..9190)
                     /gene="nrdH"
                     /locus_tag="QBH27_004008"
     CDS             complement(8945..9190)
                     /gene="nrdH"
                     /locus_tag="QBH27_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDQ7016265.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(9437..9766)
                     /locus_tag="QBH27_004009"
     CDS             complement(9437..9766)
                     /locus_tag="QBH27_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDQ7016266.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            9918..>10254
                     /locus_tag="QBH27_004010"
     CDS             9918..>10254
                     /locus_tag="QBH27_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDQ7016267.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGE"
BASE COUNT         2554 a         2623 c         2634 g         2443 t
ORIGIN      
        1 tgacgggcca tacaaaacat ggcgaacatc cattgccatg acggtcagtg cagcaaccca
       61 caaactactc ccggctgcca gcatcgcggt aatgacgaac tggctcgcgc ctgcataaat
      121 gatgcaggag aaaaaaacgc tttcgagagg agagaatccc agacgggtcg cattcagacc
      181 gaacgcaaag gccaccggaa tataactaat aacaatcggt aaactgtctt tgcatccttc
      241 catgaaggtc gccgaaccag gagcaggctg tggagtaggg ctttccatag agtgtacgct
      301 taacgattgt taatattata aatagactga atgaatatct taaccttatc agactgatgg
      361 gcttcttaac acccttataa gtgtaaagcc acgaaaacgg ttgctgattg cttaatttgc
      421 ggcttcctga cgatgaaaac ctcgccacca gaccagtaag ttaaccaggg caatcgtcgc
      481 gccagccaga cacacgccag cccaactgcc atgttgccag gctgaggctg aaattagcga
      541 accagcggca ccgccaataa agtagctggt catgtaacct gcggtcaggc gattacgcgc
      601 atcagggtgt attcgataaa ttaccgtctg gttagtgata tgcacgccct gcacggtgag
      661 gtccagcacc aggatgccga taatcagcgc cagtacggaa gtgtgaccaa accagattgc
      721 cagccatgaa agtaacagca gaagcagacc gaaagttgtt gtgtggtgcg atttgccctt
      781 gtcggcaaaa ccgcccgccg gacgagcgcc caacgctccg gcagctcccg caagtccaaa
      841 cagaccaatt acaccatcgc tgtagttaaa aggtggagcg gcaagcaaaa aggccattga
      901 ggtccagaga atgctgaagt tggcaaaggt caggcagccc agcaacgcgc gggtgcgcag
      961 gatcttatcg ctgataaaca tgctgaaaac ggaaccaagc aactgtgggt agttgaggtg
     1021 ggtttctgat ttcatttgtg gcagaccacg ccataatgcc agcgccatca gtgccattaa
     1081 caccgaggca acccaaaaga cggtgcgcca gccgccgagg tttgccagca atccggcaac
     1141 ggtccgtgcg agtaagatcc ccaatagcag gccgctcata atggtgccaa ccactttgcc
     1201 gcgtttgtcc ggtgaggcca gcgtcgctgc cagtggaacc agaatttgtg ccacgaccga
     1261 gaataaaccg gttaatgccg taccgaggat catcatcgcc agtgactgac tgctggcagt
     1321 gattaacata ccgcccgctg ccagtaaggt catcgagaca atcaggcggc ggcgttcaaa
     1381 catatcaccg aggggaacaa gaaacagcag acctgcggca tagcccaact gcgcggcggt
     1441 aacaataaag cccgccgaac tggcggaaag ggaaaagtta cgcgcgatgg tgtcgagcaa
     1501 tggctgggcg taatagttac tggctaccgc cagaccggtg gcgatagaca tcagcacgat
     1561 cagcgccggg ctaagctcat gattaggttt agtcattatt tttgttcatc aggtgatgat
     1621 taagctggaa atcaataata gcggatgttg atgaatatgg gatgaaagat agattgttga
     1681 aatgtgcggt gtggtttgta ggtatgataa gacgcgccag cgtcgcatca ggcattgtgt
     1741 acagagcggc ggatgaggca aaaacgcctt atccgcccga ataaaaatta cttctgcgct
     1801 gccagcgctt cattcaccca gccatcgaac tgctgctggt gggctttgat ccaaccatca
     1861 acgtggccct gaatatcgtc ttctgaggct ttgccgtcat gcatgatggc gttctgggcg
     1921 ttaatatctg ccactggtaa ctgcataatg gcaaacagtt tcgctgctgc cgggtttttc
     1981 tcggcccagg ctttgttggc aacgatatgc atggtactga ccgggaagcc gtaattcgcg
     2041 ccattcggca gtttggtatt ggcattttta tcgcccggca gtgcggagaa cggcacctgc
     2101 aaccagacca catctttccc cggtttcagt tcgttactca cccagtacgg cgtccaggtg
     2161 tagtaaaaca ccggcttacc ctctttgtaa cgactgatgg tgtcagccat catcgctgcg
     2221 tagttcccct gattatgcgt cacggtattg gtcagtccat acgcggcaag ctggtggttg
     2281 atcgcacctt cgcagcccca gcctgggtta cagccggtta aatccgcttt accgtcgccg
     2341 ttggtatcga acagtttggc gatcttcgga tctttaagtt gcgcgatgtt ggtaatttta
     2401 tactggtcgg cggttttctt atcgatcagg taaccttgtg ccgcgccgtt aacaaatacc
     2461 ccttcacgat aaaatttctt atcgccaccg gcagcttcgt acatgttgtc atgcagtggc
     2521 gtccagttca cggcggtgaa ggttgcatcg ccggaagcaa gcgaggtgta gccaacgttg
     2581 taatccactt cgctgggttt attgacggta taacctaatt tctccagcgc gcggctgacc
     2641 agcagcgtct ggaaggtttc ttcggtgatg gtgctttgaa ctggattaac tgtaatgcct
     2701 ttgcccggca ggtcggcagc aaaagtttgt gtagagataa gcgtggcaaa cgctgtcgca
     2761 aaaagtacgc tatgtcgcat tgttattcct ttttttggca gggtgatatg cccggtgtta
     2821 ccgggcaagt gcagagttac ttaatgaatg ggcgggtcag cagaccgaca gggccagtgg
     2881 tgtaccagcg acggttgccg cgactacgtg agtcgcgccc aacggcctgc gtcaggcgat
     2941 cgaggataat ggcgaggatc acaatcccga cgccgccaac ggtggcaagc cccatatcca
     3001 gacgaccgat accgcgaagt accatctgac ccagcccgcc gacggcaatc atcgaagcga
     3061 tgaccaccat agaaagggcc agcatcagcg tctggttaac gcccgccata atggttggca
     3121 tcgccagcgg taactgaact ttgaacagca tctggctcgg gctggcaccg aatgagcgcg
     3181 aggcttcaat cagatccgcc ggaacctggt taattcccag aatcgtcaga cggataatcg
     3241 gcggcagtgc aaagatgatc gtcaccacca cgcccggcac gttgccgata ccaaacagca
     3301 tgacgattgg caccagataa acaaacgcgg gcgtggtctg catggcatca agcagtggac
     3361 gaataatttt cgccgctcgc ggacttctcg ccagccagat ccccaacggc aaacctatga
     3421 cgatacagaa cagcagggcg gttaacacca gcgccagggt aaccattgcc tgtgaccagg
     3481 caccgattgc gccgatggca atcagcgaaa ccagcgtcgc cacgcccatt ccgaccccgg
     3541 aaatctgcca ggcgatgaga gcgaaaacga taatcgccac cggcgcgggc atacccagca
     3601 gcaattgctg gaaaccgttg aggatgtaat caaccggaac gcgcacgccc tgaaagacgg
     3661 gacgaaaatg ggtaacgacc cagtcgatcc cttcagtgac ccaactgtcg agcgggatca
     3721 gagttttatg gaacggatcg agaatattaa aatgctcgac gtttggcgca ggcgtactgg
     3781 tcagccagtc agcaccaccg ccgtcagtcg gtgcagcagc cggtgtaccc caggcgtctg
     3841 cggattgtgc agcactgtcc gccgctggcg tggtatccca cggattattt tgatcagcca
     3901 ttatttaccc cctcacgatc taaagcgcgc agcagcattc ctttcgaaat gatgccgaca
     3961 tactgttggt cctcgtcgac cacgggcacc gcacagggtg cctgtccgac atgagagagc
     4021 aactcgctaa gaggcgtttg tgcatcgacc gctaacggcg catcaatcag cgccgcatca
     4081 agaccttgct gctgcgttaa cgcggcttta agcgaatcga tagacacagc gccgacaaac
     4141 ttattaccgc gttcgatgac gtagccatat tcgcgatctt catcctgcaa taatttcagt
     4201 gccgaacgcg ggccgaagcc gggggtttta cgaattaagc cgttcggtgt acgacgagca
     4261 atatctttcg cactgaacac ctggctaata tcgacgccac ggaagaaggt acgaacataa
     4321 tcattcgccg gattattaag aatttcatcc ggtgtaccaa cctgcaccac ttcaccattt
     4381 tgcataatgg caattcggtc accaatacgc atagcttcat cgagatcgtg ggaaataaag
     4441 acaatggtgc gctgatgttt cgcctgtaat tttaccagct catcctgcat ctcggtgcga
     4501 attaatggat cgagtgccga gaaggcttcg tccattaata atatatccgg attaatcgct
     4561 aacgcgcggg ctaatcccac acgttgacgc atcccgccag agagttcatc cgggtagctg
     4621 tgggcataat tttccagccc gacctgacgc agtgcatcaa gggctttttc ccggcgttct
     4681 tcggcattaa ttccggccag ttccataccg aacgcagtat tgtccagcac ggtcatatgc
     4741 ggcattaagg caaaggactg gaagaccatc gcaatctttt ttctgcgcac ctcacggagt
     4801 tcgacgtcgg atattttggc aatatccaca ccatcaatca gcacttgccc gcgggtgggt
     4861 tcaatcaggc gattgagaag gcgtaccatt gtggatttac ccgaaccgga taatcccatg
     4921 atgacaaata tctcgccttc ttcaatggcc agactggcgt cttttacgcc aagcgatagc
     4981 ccagtttttt ccagaatttg ttcttttgaa agtccttgtt cgatatattt gaaagctcgc
     5041 tgtggatgct caccaaatat tttataaaga tttttaattt ctaatttaat tgccatgcaa
     5101 tagaaagatt cctgttattt gtttatgtcg atacattacc caatggaaat agtcactttt
     5161 ttctacccta acatactgag aatctgaggc aacccctgat ggcaaatgtg gcatgaatat
     5221 tgatgtgagc aaatcgccgg aatttcccat gatataaggg ctgagagcaa atcgagaaaa
     5281 gttgatattt tttgtcaaag atagcaaaaa aacgcctgaa tattgtggtt tcaggcgaat
     5341 aatgcaacag agtatcagtg tagatcacca caaattattt gcgtgatata aagctatttg
     5401 gcgggaatta tttccctgca gcgggtagtg atatttttga aaataacccc ctcagaaatt
     5461 ccagtcttca tcttctgttt cgaccgcttt tcccatcaca tatgaagagc ctgaaccgga
     5521 aaagaaatcg tgattttcat cggcattcgg cgaaagcgcg gcgaggattg ccggattcac
     5581 ttccgccatt tctgcgggaa atagcggttc gtagcccaga ttcatcaacg ccttattggc
     5641 gttgtaacag agaaacgctt tcacatcgtc agcccacggg gtttcggcgt acagctcatc
     5701 ggtgtattgc aactcgttgt catagagttc cagcaacaaa tcgaaggcga aactcttcaa
     5761 ttcttcacgt cgtgcctggg agatcttttc caggtttttc tgatatttat agccgatgta
     5821 gtaaccgtgg actgcttcat cccggataat cagacggatc aggtctgcgg tattggttag
     5881 ctttccgcgg ctggaaaaat acatcggcag ccagaaaccg gaatagaaca aaaaagattc
     5941 aagaaacaca ctggcgattt tctttttcag cggatcatca ccgcgataat gttgctgaat
     6001 aatctgtgct tttcgctgca acggcgcgtt ttcttcactc caggcgtagg cggcatcgac
     6061 atctttggtc tggcacagcg tcgagaaaat tgaactgtaa gagcgggcat gaaccgcttc
     6121 cataaagctg atatttgata ataccgcctc ttcatggggg gtgagtgcat cgggcatcag
     6181 cgagggcgcg ccgataacat tttgcagcgt gtcgagcagc gtcaggccag taaaaacgcg
     6241 cattgttagt tgttgttcta cgacacttaa tgtctgccat gcaggaatat cgttcgacag
     6301 tggcaccttt tctggtagcc agaaattgct ggtcaggcga ttccacacct ccagatcttt
     6361 atcgtcagat atcttgttcc agttgatggc gctgatacgt gagagtttca tagatattcc
     6421 ttaaagtgca caggagacgc agccttcaat ttcagtgcct tccagcgcca tctgacgcag
     6481 gcgaatgtaa tagagcgttt tgataccctt gcgccaggcg taaatctgcg ctttgttgat
     6541 atcgcgagtg gtggcggtat cggggaaaaa cagcgtcagc gacagcccct gatcgacatg
     6601 gcgagtcgct tccgcgtagg tgtcgatgat cttttctgcg ccgatttcgt aagcgtcctg
     6661 atacagcgcc agattttcgt tagtcataaa cggggcaggg tagtaaacgc gcccggtttt
     6721 gccctctttg cgtatctcta ctttcgccac aatcggatga atactcgacg tggcatggtt
     6781 gatataagag atagaaccgg ttggcggcac cgcctggaga ttctggttgt agataccata
     6841 gcgcatcacg tcgtcgcgca gctgcgccca catctcacgg gtaggtaacg taataccgct
     6901 acgggcaaac agttcgccaa ctttcgccgt tttcggctgc cagttacttt gcagatattg
     6961 gctgaaatat tcaccactgg catagcgtga ctgtttgaac ccggcgaagg tttcaccgcg
     7021 ttcgcgcgcc agcaacatcg aggtacgcag tgcgtgccaa gtgatggtat agaaatagag
     7081 attggtgaaa tcgagtgctt ccggcgaacc ataagcgatg ccttctcgcg ccagatagcc
     7141 gtgtaaattc atctgcccca gtccgatggc gtgcgaggcg gcatttccgg cttcgatgga
     7201 cggcacgctg cggatatgac tcatatctga caccgccgtt aaaccgcgta cggcagtctc
     7261 taccgtgcgg gcaaagtcgg gggaatccat ggtgtgggca atattcaacg accctagatt
     7321 gcaggaaata tcatggcctg tacgggcata gtcgagattc tcgtcatatt ctgaggcgct
     7381 gttaacctgc aaaatttctg agcagagatt gctcatattt atgcgcccgg cgatagggtt
     7441 agcacggtta accgtgtctt catacatgat gtacggatag ccagactcaa actggatctc
     7501 tgccagtcgc tggaagaaat cacgagcgtt gaggtatttt ttgcgaatgc gttcatcggc
     7561 aaccagttcg tcatagcgtt cgctgatggc gacatcggca aacggtttgc cataaacccg
     7621 ctcaacgtca tagggcgaaa acagcgccat ctgcgcattc tcttttgcca gatggaaagt
     7681 gatatccggg atcaccacgc ccagcgacag tgttttaatg cggatttttt cgtcggcatt
     7741 ttcccgtttt gtatcgagaa aacgcagaat atcgggatga tgagcatgta aatagactgc
     7801 cccagccccc tgacgcgcgc cgagttggtt ggcataggaa aatgcgtctt ccagcatttt
     7861 catcaccgga atcacgccag aagattgatt ttcaatacgt ttaattggcg cgcccgcttc
     7921 ccgcagattc gacagcaaaa atgctacgcc gccgccgcgt ttcgacagtt gtagtgcgga
     7981 atttactgcc cggccaatcg actccatatt gtcttcaata cgcagcagaa aacaggaaac
     8041 cagttcgccg cgctgctgct taccgcagtt gaggaatgtt ggcgtggctg gctgaaagcg
     8101 tcctgacagc atctcatcgg tgagctgcgt cgccagtgtt tcatcgcctt gtgccagcgt
     8161 cagcgccacc atcgtgacgc gatcggcaaa atcttccaga taacgcttac cgtcgaatgt
     8221 cttcaacgta tagctggtgt aaaacttcca tgctcctagg aatgtctgga aacgaaaacc
     8281 gctggcgtgc gcgtgggcaa acagcgtaat gacaaaatcg cgagagtagc gattaaggac
     8341 actttcatca tagtaacctt cgttaaccag ccagttcagg cgctgctgct ggctactgaa
     8401 ggccacactg ttcggacgca catgcgtcgt cataaaggcg tctactgcct ggcgatcttt
     8461 atcgaactga atgcgacctg cgctatcgta aaggttaagc atcgcattca gagcgtgata
     8521 atccatcgtt tcctgcgtca ggcgttctgc ggttgtcgtt gccaaaattc agttactcct
     8581 ttacgaacgt tttcgatatc gctttgcgta cccatgagtt caaagcggta cagccacggc
     8641 acgccgcatt tccgggcaat cacatctccg gcgcggccat atgcctcacc aaagttgcga
     8701 ttacccgaag caataacgcc gcgcagcaac gcccggttat gctcgtcgtt taaaaagcga
     8761 atgacctgtc gtggcaccgc gccagccgta ccgccgccgc cgtaagaggg cacgatcagg
     8821 atgtaaggct cgtctacctg aatccgttcc cgctcattga gcgggatgcg caccgcgggc
     8881 agccctaaac gttcgataaa acgctgcgta ttttcggagc tgctggaaaa gtagacgagc
     8941 tggctcatgc actggccgcg tgtggcgctg gatgcagacg attgatcatg tccgggcgga
     9001 aaccagacca gctaagatcg cctgcaatca caaccggcaa ctgacgaaag ccctgagcac
     9061 gcagcgcttc tgccgcttca ggaacgcgat cgacattaat catttcaaaa tcaaagcccc
     9121 ggttttccat cgcccgttta gtggcgtggc actgaacgca atcgttacga gtgtaaatag
     9181 taatgcgcat gattcgtatt tccgtttaaa atgaagatac ggcgcgatga tacgcgtcgg
     9241 gttgtctctc tgttgataca gagatactag atgtagttga aaaaagattc aaccacacaa
     9301 tatatagcaa ataatggtca gggttaccct ggggcgagcg tgacggggta gggggatttt
     9361 tgtgattcac caggcaaaaa gaaaccccga agccaggctt cggggtcaaa gacgcgtatt
     9421 tattatcatt tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccca
     9481 ccgctgccgc tgtacccaca ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc
     9541 caacggcatc gcgcgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt
     9601 ctttcagtag agcctgcgct ttactacgag cggcttcggc ttcccctttg gcgtcgctgc
     9661 cccaggactt caatacagat tcgagactgt cagctaattg attgacatca ttctggatat
     9721 cctgcacgcc atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta
     9781 gaattcgatg taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt
     9841 tatggacttt tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat
     9901 aataacgagg atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag
     9961 tcggttttac tgtcgatgtg gtaacccaga aagcgtatgg ttaccgccgt ggggaaaatt
    10021 atgtctatgt taatcgcgaa gcgcgaatgg gacgtaccgc cctggtgatt catccaacat
    10081 taaaagaacg tagttcaaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt
    10141 accagcaatt tccgctctat ttagcgggtg aacgtcatga gcattacggt atcccgcatg
    10201 gctttagttc gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaag
//
LOCUS       DAOFSV010000157         9873 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.157, whole genome shotgun sequence.
ACCESSION   DAOFSV010000157 DAOFSV010000000
VERSION     DAOFSV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.157"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(40..1005)
                     /gene="trxB"
                     /locus_tag="QBH27_004011"
     CDS             complement(40..1005)
                     /gene="trxB"
                     /locus_tag="QBH27_004011"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDQ7016268.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            1549..2043
                     /gene="lrp"
                     /locus_tag="QBH27_004012"
     CDS             1549..2043
                     /gene="lrp"
                     /locus_tag="QBH27_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDQ7016269.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            2178..6284
                     /gene="ftsK"
                     /locus_tag="QBH27_004013"
     CDS             2178..6284
                     /gene="ftsK"
                     /locus_tag="QBH27_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDQ7016270.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQSTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAAEQPAQQPYYAPA
                     PEQAVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVVPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            6443..7054
                     /gene="lolA"
                     /locus_tag="QBH27_004014"
     CDS             6443..7054
                     /gene="lolA"
                     /locus_tag="QBH27_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDQ7016271.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            7065..8408
                     /gene="rarA"
                     /locus_tag="QBH27_004015"
                     /gene_synonym="mgsA"
     CDS             7065..8408
                     /gene="rarA"
                     /locus_tag="QBH27_004015"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDQ7016272.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            8499..9791
                     /gene="serS"
                     /locus_tag="QBH27_004016"
     CDS             8499..9791
                     /gene="serS"
                     /locus_tag="QBH27_004016"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDQ7016273.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         2426 a         2491 c         2652 g         2304 t
ORIGIN      
        1 tggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt cagctaaacc
       61 gtcgaggtag cgttccgcat caagagctgc catgcagcct gtaccagcag atgtaatggc
      121 ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag acgccaggga tgctggtctg
      181 ggtggcatta ccatgaatac ccgactgtac tttgatgtag ccgttttcca gttccagctg
      241 cccttcgaaa atggcagtat tcgggctgtg accgatagca acaaacagac cggcaacgtc
      301 gagtgactcg atattatcgg tgttttgcgt atcgcgcaga cgaacgccag tgacgcccat
      361 ctgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaataatgt tgccgttctc
      421 cactttatcc atcagacgct taatgaggat tttttccgca cggaaaccat cacggcggtg
      481 aatcagatga acttccgaag cgatgttaga cagatacagc gcctcttcaa ccgcggtatt
      541 gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca
      601 agcagaaact ccacggcctt taaaggcttc ttcagagggc aggccgagat agcgtgcaga
      661 agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgctatcgc cagtcagacg
      721 gaacggacga ttctgcaaat caactttgtt gatgtgatca aagatgattt cagtttcaaa
      781 tttggtggca tgttcgtgca tgcgctccat taataacgga ccggtcagat cgtttggatc
      841 gccaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt
      901 aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg
      961 gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat ccccatagtt
     1021 gttggcagac aatggcagga ttgtagggaa tttacagacg taaaaaaaga gtatgacgat
     1081 tttgttaaca atttgtgcaa tcggcagcat cgataagcag gtcaaattct cacgtcatta
     1141 tcacctctgc tacttaaatt tcccgcttta taagccgatt aaatgatgaa taaacgcccc
     1201 tgttaatgaa tatctggcat gttgtactaa aaatcgatgt tttgctttga caatcccctg
     1261 gtgttttgcg aaaacattcg aggaagaaaa aaaacagtat tcttatatgc gcataaccat
     1321 gcatgtaaat accatgttta ccgtgctagt gaaatctacg tatggcgtgg acagacgcca
     1381 ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc accgtagacc caggcattgc
     1441 gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt agcgactctg aacagtgatg
     1501 tttcagggtc agacaggagt agggaaggaa tacagagaga caataataat ggtagatagc
     1561 aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta acattcttaa tgagttgcaa
     1621 aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg tgggactttc cccaacgccg
     1681 tgccttgagc gtgtgcgtcg gctggaaaga caagggttta ttcagggcta tacggcgctg
     1741 ctgaaccccc attatctgga tgcatcactt ctggtattcg ttgagattac tctgaatcgt
     1801 ggcgcaccgg atgtgtttga acaattcaat accgctgtac aaaaacttga agaaattcag
     1861 gagtgtcatt tagtatccgg tgatttcgac tacctgttga aaacacgcgt gccggatatg
     1921 tctgcttacc gtaagttact gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca
     1981 cggacatacg ttgttatgga agaagtcaag cagagtaatc gtctggttat taagacgcgc
     2041 taacacggaa caggtgcaaa atcggcgtat tttgattaca ctcctgttaa tccatacagc
     2101 aacagtactg gggtaacctg gtactgttgt ccgttttagc atcgggcagg aaaagcctgt
     2161 aacctggaga gcctttcttg agccaggaat acactgaaga caaagaagtc acattgacaa
     2221 agttaagtag cggccgccgc cttctggagg cgttgctgat ccttattgtc ctgtttgccg
     2281 tctggttgat ggctgcctta ctaagcttta acccttcgga ccccagctgg tcgcaaacgg
     2341 cctggcatga acctatccat aatttaggtg ggatgcccgg tgcgtggctg gcagatacgc
     2401 tgttctttat ttttggcgtg atggcttaca ccattcccgt cattattgtc ggcggttgtt
     2461 ggtttgcctg gcgtcatcag tccagcgacg aatacattga ttattttgcc gtttcgctac
     2521 gcatcattgg cgttttggcg ctcatcctta cctcctgtgg tctggcggca atcaacgctg
     2581 acgatatctg gtattttgcc tccggtggcg tcattggcag cttactaagc actacgctac
     2641 aaccactgct acacagtagc gggggaacta ttgcgctgct ctgcgtttgg gcagcgggcc
     2701 tgacgctgtt caccggttgg tcatgggtga ccattgctga aaaactcggc ggctggattt
     2761 taaatattct taccttcgcc agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag
     2821 atgagtatga agacgacgaa gagtatgaag atgaaaatca cggcaaacag catgaatcac
     2881 gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca
     2941 ttaatccgat ggggcggcaa acagacgctg cgttgttctc cggtaagcgg atggatgatg
     3001 acgaagagat tacctacact gcacgcggtg tggctgccga ccctgacgac gtcctctttt
     3061 cgggcaatcg tgcaacgcag ccagaatatg acgagtacga tccattatta aacggtgcgc
     3121 caattaccga acctgttgct gtagcagctg ctgctaccac ggcgacacaa agctgggctg
     3181 caccggttga acctgtgact cagacgccgc cagttgcctc tgttgatgtt ccacctgcgc
     3241 aatctacagt agcctggcag cctgtaccgg ggccgcaaac gggagaaccg gttattgctc
     3301 ctgcaccgga aggttaccca cagcagccac aatatgcgca gcctgcagtg caatataatg
     3361 agccgctgca acaaccagta cagccacagc agccgtatta tgcacctgca gctgaacaac
     3421 ctgcgcaaca accttactac gctcctgcgg cggaacaacc tgttcagcaa ccgtattacg
     3481 caactgcggc ggaacagcct gcgcaacagc cgtattacgc ccctgcgcca gaacaagcgg
     3541 tggcaggtaa cgcctggcaa gccgaagagc agcaatccac ttttgctcca cagtctacat
     3601 accagactga gcaaacttat cagcagccag ccgctcagga accgttgtac caacagccgc
     3661 aacctgttga acagcagcct gttgtggagc ctgaacccgt tgtagaagag acaaaaccca
     3721 cgcgtccgcc gctttactac tttgaagaag tggaagagaa gcgagcccgt gaacgcgaac
     3781 aacttgcggc ctggtatcaa ccgattccag aacctgttaa agaaccagaa ccgatcaaat
     3841 cttcgctaaa agcaccttct gttgcagcag tacctccagt ggaagccgct gccgccgttt
     3901 ctccgctggc atctggcgtg aaaaaagcga cactggcgac gggggctgcc gcaaccgttg
     3961 ccgcgccagt cttcagtctg gcaaatagcg gtggaccgcg tccgcaggtc aaagagggga
     4021 ttggcccgca gttgccacga ccgaaacgca tccgcgtgcc aactcgtcgt gaactggcgt
     4081 cttacggtat taagctgccc tcacagcgtg cggcagaaga aaaagcccgt gaagcccagc
     4141 gtaatcagta cgattctggc gatcagtaca acgatgatga aatcgatgcg atgcagcagg
     4201 atgaactggc acgtcagttc gcccagacgc agcagcaacg ctatggcgaa cagtatcaac
     4261 atgatgtgcc cgtgaacaca gaagatgcag atgctgcggc agaggcagaa ctggctcgtc
     4321 agtttgctca aactcaacaa caacgttatt ccggcgaaca accggctggg gcgaatccgt
     4381 tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg
     4441 aaccgctgtt tacgccaatt gttgaacctg tacagcagcc gcaacaaccg gtagcaccgc
     4501 agcagcaata tcagcagccg caacaaccgg ttgcaccgca gccgcagtat cagcagccgc
     4561 aacaaccggt tgcaccgcag cagcaatatc agcagccaca acaaccggtt gcacagcagc
     4621 cgcagtatca gcagccacaa cagccggtta cacagcagcc gcagtatcag cagccgcagc
     4681 agccggttgt accacagccg caatatcagc agccgcaaca gccagttgcg ccacagccgc
     4741 aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg ttgcataaac
     4801 cgacgacgcc actgccttct ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg
     4861 tagatacctt tgcgcttgaa caaatggcgc gcctggtaga agcgcgtctg gctgatttcc
     4921 gtattaaagc cgatgttgtc aattactctc cggggccagt tatcactcgc tttgaattga
     4981 acctggcacc gggcgtaaaa gcggcgcgca tttctaactt gtcacgtgac cttgcccgtt
     5041 cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc
     5101 tggagttacc gaataaaaaa cgacaaaccg tttatctgcg cgaggttttg gataacgcca
     5161 aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc gccggtgagc
     5221 cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact accggttccg
     5281 gtaagtctgt cggggtgaac gcgatgatcc tgagcatgct ttataaagca cagccagaag
     5341 atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat gaaggcattc
     5401 cgcacctgtt aacggaagtt gttactgata tgaaagatgc cgccaacgcg ctgcgctggt
     5461 gtgttaacga gatggagcgt cgttataaac tgatgtctgc gctgggtgtg cgtaatctgg
     5521 cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt ccagacccgt
     5581 actggaagcc gggtgatagt atggatgcgc agcatccggt gctgaaaaaa gaaccgtaca
     5641 tagtggtgtt ggtggacgaa tttgccgacc tgatgatgac ggtaggtaaa aaagtggaag
     5701 agctgatagc acgtctggcg caaaaagctc gtgccgcggg tatccacctc gtactggcaa
     5761 cgcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt ccgacccgta
     5821 tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag gctggtgcgg
     5881 aatcactgct gggtatgggg gatatgctct actctgggcc gaactccact ttgccggtac
     5941 gtgtccatgg tgcttttgtt cgcgatcaag aagttcatgc cgtggtgcag gactggaaag
     6001 cgcgtggtcg cccacagtat gttgatggca tcacctccga cagcgaaagc gaaggtggcg
     6061 cgggcggttt cgatggcgct gaagagctgg atccgttgtt cgatcaggcg gtgcagtttg
     6121 tcactgaaaa acgcaaagca tcaatttctg gcgtacagcg tcagttccgc attggttata
     6181 accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc gaacaggggc
     6241 acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg catcgtaggc
     6301 cggataaggc gcggtagcgt cgcatccggc aatctggtaa ctgaaaattc agtattttct
     6361 tctttcctca agctgattat tagcctggaa tagagagtag agggagctcc cggtcgggag
     6421 tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat tactctcaag
     6481 cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa
     6541 agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg gcgcggcggt
     6601 gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact ggcatatgac
     6661 tcaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct ataacccgtt
     6721 cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc cgtttatgct
     6781 gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga atggcgatga
     6841 ctttgttctg acgccgaaag ccagcaatgg caatctgaag cagttcacca ttaacgtggg
     6901 acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc gcagcagtta
     6961 tcagctgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct tcaccccgcc
     7021 gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc
     7081 tcgatttttc ggataatact tttcaacccc tggccgcgcg tatgcggcca gaaaatttag
     7141 cacagtatat cggccagcaa catttgctgg ctgtggggaa gccgttgccg cgcgctatcg
     7201 aagccgggca tctgcattct atgatcctct gggggccgcc gggtaccggc aaaacaaccc
     7261 tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct gccgtcacct
     7321 ctggcgtgaa agagattcgc gaggcgatcg aacgcgctcg gcaaaaccgc aatgcaggtc
     7381 gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag caggatgcat
     7441 ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact gaaaacccgt
     7501 cgtttgagct taattcggca ctgctttccc gtgcccgtgt ctatctgttg aaatccctga
     7561 gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacc cgtggctatg
     7621 gtggtcagga tattgttctg ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg
     7681 gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc gaagtcgatg
     7741 atagcggtaa gcgggtcctg aagcctgaat tactgaccga aatcgccggt gaacgtagcg
     7801 cccgctttga taacaaaggc gatcgctttt acgatctgat ttccgcattg cataagtcgg
     7861 tacgtggtag cgcacccgat gcggcgctct actggtatgc gcgaattatt accgccggtg
     7921 gcgatccgtt atatgttgcg cgtcgttgcc tggcaattgc ttccgaagat gtcggcaatg
     7981 ccgacccacg ggcgatgcag gtggcaattg cggcctggga ttgctttact cgcgttggcc
     8041 cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc gcgccaaaaa
     8101 gcaacgctgt ctacactgcg tttaaagccg cgctggccga tgctcgcgaa cgtccggatt
     8161 atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg
     8221 ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt gaggtttact
     8281 tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc cttgaaggca
     8341 agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc ataaaacgct
     8401 accgttaatg ttatcgttgc ggtaatgttg ttaatgtatc cctgtggtcg caggctgtgg
     8461 ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc aatctgctgc
     8521 gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag ctggatgtag
     8581 ataagctggg cgctcttgag gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc
     8641 aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata
     8701 tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg
     8761 agctggatgc tttacaggct gaaattcgcg atatcgcgct aaccatccct aacctgcctg
     8821 cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagt cgctggggca
     8881 ccccgcgtga gtttgacttt gaagttcgtg accatgtgac gctgggtgaa atgcactctg
     8941 gcctcgactt tgcagccgca gttaagttga ctggttcccg ctttgtggta atgaaagggc
     9001 agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat accgaacagc
     9061 atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg tacggtacgg
     9121 gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa gaagaagcag
     9181 acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgaccaac ctggtacgcg
     9241 gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc ccatgtttcc
     9301 gttctgaagc tggttcatat ggtcgtgaca ctcgtggtct gatccgtatg caccagttcg
     9361 acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg ctggaagaga
     9421 tgaccggtca tgcggaaaaa gtcctgcagc tgctgggcct gccgtaccgt aaaatcatcc
     9481 tttgtaccgg cgacatgggc tttggcgctt gcaaaactta tgacctggaa gtatggatcc
     9541 cggcacagaa cacctaccgc gagatctctt cctgctccaa cgtttgggat ttccaggcac
     9601 gtcgtatgca ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg gttcataccc
     9661 tgaacggttc tggtcttgct gttggtcgta cgcttgttgc agtaatggaa aactatcagc
     9721 aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaat ggactggaat
     9781 atattggcta atacccaatt tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc
     9841 tccctttgat accgaacaat aattactcct cac
//
LOCUS       DAOFSV010000158         9807 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.158, whole genome shotgun sequence.
ACCESSION   DAOFSV010000158 DAOFSV010000000
VERSION     DAOFSV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.158"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            121..1095
                     /gene="cysB"
                     /locus_tag="QBH27_004017"
     CDS             121..1095
                     /gene="cysB"
                     /locus_tag="QBH27_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDQ7016274.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1390..1554
                     /locus_tag="QBH27_004018"
     CDS             1390..1554
                     /locus_tag="QBH27_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDQ7016275.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
     gene            1557..1724
                     /locus_tag="QBH27_004019"
     CDS             1557..1724
                     /locus_tag="QBH27_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016276.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1838..1933
                     /locus_tag="QBH27_004020"
     CDS             1838..1933
                     /locus_tag="QBH27_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016277.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2083..4758
                     /gene="acnA"
                     /locus_tag="QBH27_004021"
     CDS             2083..4758
                     /gene="acnA"
                     /locus_tag="QBH27_004021"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDQ7016278.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSFKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVFLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCHCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4822..5412)
                     /gene="ribA"
                     /locus_tag="QBH27_004022"
     CDS             complement(4822..5412)
                     /gene="ribA"
                     /locus_tag="QBH27_004022"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDQ7016279.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5582..6346
                     /gene="pgpB"
                     /locus_tag="QBH27_004023"
     CDS             5582..6346
                     /gene="pgpB"
                     /locus_tag="QBH27_004023"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDQ7016280.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6495..6803
                     /gene="lapA"
                     /locus_tag="QBH27_004024"
     CDS             6495..6803
                     /gene="lapA"
                     /locus_tag="QBH27_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDQ7016281.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6810..7979
                     /gene="lapB"
                     /locus_tag="QBH27_004025"
     CDS             6810..7979
                     /gene="lapB"
                     /locus_tag="QBH27_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDQ7016282.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKAEYAKAVESLQRVISQDRELVSETLEMLQTCYQLLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8171..8908
                     /gene="pyrF"
                     /locus_tag="QBH27_004026"
     CDS             8171..8908
                     /gene="pyrF"
                     /locus_tag="QBH27_004026"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDQ7016283.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRDFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEACDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8908..9234
                     /gene="yciH"
                     /locus_tag="QBH27_004027"
     CDS             8908..9234
                     /gene="yciH"
                     /locus_tag="QBH27_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDQ7016284.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(9360..9578)
                     /gene="osmB"
                     /locus_tag="QBH27_004028"
     CDS             complement(9360..9578)
                     /gene="osmB"
                     /locus_tag="QBH27_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDQ7016285.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2444 a         2277 c         2539 g         2547 t
ORIGIN      
        1 aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa atcgtattag
       61 tcacccgcct ggtgtggcga aaaaacgatg ttctgatggc gtctaagtgg atggtttaac
      121 atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct gaatgtctca
      181 tcaacggcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt cagaatgctg
      241 gaagacgagc tgggcattca aattttttcc cgaagcggca agcacctgac gcaggtaacg
      301 ccagcaggac aagaaataat tcgtatcgct cgtgaagttc tgtcgaaagt cgatgccata
      361 aaatcggtcg ccggagagca cacctggccg gataaaggct cgctgtatat cgccaccacg
      421 catactcagg cacgctatgc gttaccaaac gttatcaaag gctttattga gcgttatcct
      481 cgcgtttctt tgcatatgca ccagggctcg ccgacacaaa ttgctgatgc cgtctctaaa
      541 ggcaatgccg atttcgctat cgccacggaa gcgctgcatc tgtatgaaga tttagtgatg
      601 ctaccgtgct accactggaa tcgggctata gtagtcactc cggatcaccc gctggcaggt
      661 aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata taccttcggc
      721 tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac gccgcgtatc
      781 gtcttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg gctgggggta
      841 ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt gcgtgttgat
      901 gctcacgaca tcttcagcca cagtacaacc aaaattggtt ttcgccgtag tacttttttg
      961 cgtagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt
     1021 gatgcggctg tcgcattgcg ctctaatgaa gaaattgagg tcatgtttaa agatataaaa
     1081 ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg
     1141 aatatattct cctttgttac ttatttgtat ccggtgcaca atatttttat ccctcatatt
     1201 tatagggtaa attacctgta aagtagtgtc taattacaca cctcgcaaac tccagttttc
     1261 aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg taaattcgct
     1321 ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt acaccaatcg
     1381 ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc ttctggaaat
     1441 caggaacccc gccgcaatcc tgaattaaaa cgtaaagcct ggctggcggt ttttcttggt
     1501 tctgcacttt tctgggtggt tgtcgcactg cttatttgga aattgtgggg ataaccatgg
     1561 tcggtcagga gcaactggag tcgtcaccat tatgtcagca tagtgacaat gaaccagaag
     1621 caaaacggga atgttccgtc gttattccag acgactggca actaacatcg cagcaacaag
     1681 cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca tcattcttat
     1741 tacccatttt taatgaatta cagggctttt aatacaccgc agcaataaca ggttgagtta
     1801 tctcaacaca aagtaataac cgttaagggt gtagcctatg atcaacacaa atatgaaata
     1861 ttggtcctgg atgggcgcat tttctctgtc gatgctcttc tgggccgaac tcctctggat
     1921 cattactcac tgatccctga tcccgctgtt tgctttgcct caaggtttct cctcttttat
     1981 caatttgggt tgttatcaaa tcgttacgcg atgtttgtgt tatctttaat attcaccctg
     2041 aagagaatca gggcttcgca accctgtcat taaggaggag ctatgtcgtc aaccctacga
     2101 gaagccagta aggacacgtt gcaggccaaa gataaaactt accactacta cagcttgccg
     2161 cttgctgcca aatcactggg cgatatcacc cgtctaccca agtcattcaa agttttgctc
     2221 gaaaacctgc tgcgttggca ggatggtaaa tcggttaccg aagaggatat ccacgcgctg
     2281 gcgggatggc tgaaaaatgc ccatgctgac cgtgaaattg cctaccgccc ggcaagagtg
     2341 ctgatgcagg actttaccgg cgtacctgcc gttgttgatc tggcggcaat gcgcgaagcg
     2401 gttaaacgcc tcggcggcga tactgcaaag gttaatccgc tctcaccggt cgacctggtc
     2461 attgaccact cggtgaccgt cgatcgtttt ggtgatgatg aggcatttga agaaaacgta
     2521 cgcctggaaa tggagcgcaa ccacgaacgt tatgtgttcc tgaaatgggg aaagcaggcg
     2581 ttcagtcggt ttagcgtcgt gccgccaggc acaggcattt gccatcaggt taacctcgaa
     2641 tatctcggca aagcagtgtg gagcgaattg caggacggtg aatggattgc ttatccggat
     2701 acactcgttg gtactgactc gcacaccacc atgatcaacg gccttggcgt gctggggtgg
     2761 ggcgttggtg gcatcgaagc agaagccgca atgttaggcc agccggtttc catgcttatc
     2821 ccggacgtag tgggctttaa acttaccgga aaattacgtg aaggtattac cgccacagac
     2881 ctggttctca ctgttaccca aatgttgcgc aaacatggcg tggtggggaa attcgtcgaa
     2941 ttttatggtg atgggctgga ttcactaccg ctggcggatc gcgccaccat tgccaatatg
     3001 tcgccagaat atggtgccac ctgtggcttc ttcccaatcg atgctgtcac cctcgattac
     3061 atgcgtttaa gcgggcgcag tgaagatcag gtcgagttgg tcgaaaaata tgccaaagcg
     3121 cagggcatgt ggcgtaatcc gggcgatgaa ccaattttta ccagtgtgtt agaactggat
     3181 atgaatgacg ttgaagcgag cctggcaggg ccaaaacgcc cgcaggatcg cgttgcactg
     3241 cccgatgtac caaaagcatt tgctgccagt agcgaactgg aagtgaatgc cacgcataaa
     3301 gatcgcctgc cggtcgatta tgttatgaac ggacatcagt atcagttacc tgatggcgct
     3361 gtggtcattg ctgcgataac gtcgtgcacc aacacctcta acccaagtgt gctgatggcc
     3421 gcaggcttgc tggcgaaaaa agccgtaact ctgggcctca agcggcaacc atgggtcaaa
     3481 gcgtcgctgg caccgggttc gaaagtcgtt tctgattatc tggcaaaagc gaaactgaca
     3541 ccgtatctcg atgaactggg atttaacctt gtgggatacg gttgtaccac ctgtattggt
     3601 aactcgggac cgttgcccga tcctatcgaa acggcaatca aaaaaggcga tttaaccgtc
     3661 ggtgcagtgc tgtccggcaa ccgtaacttt gaaggccgta tccatccgct ggttaaaacc
     3721 aactggctgg cctcgccgcc gctggtggtt gcctatgcgc tggcgggaaa tatgaatatc
     3781 aatctggctt ctgagcctat cggccatgat cgcaaaggcg agccggtatt tctgaaagat
     3841 atctggccat cggcacaaga aattgcccgt gcggtagacc aagtctccac agaaatgttc
     3901 cgcaaagagt acgcagaagt ttttgaaggc acagccgagt ggaaggaaat taacgtcaca
     3961 cgatccgata cctacggttg gcaggaggat tcaacctata ttcgcttatc gcctttcttt
     4021 gatgaaatgc aggcaacacc agcacctgtg gaagatattc acggtgcgcg gatcctcgca
     4081 atgctggggg attcagtcac cactgaccat atctctccgg cgggcagtat taagcccgac
     4141 agcccggcgg gtcgatatct acaaggtcgg ggtgttgagc ggaaagactt taactcctat
     4201 ggttcgcggc gtggtaacca tgaagtgatg atgcgcggca ccttcgccaa tattcgcatc
     4261 cgtaatgaaa tggtgcctgg cgttgaaggg gggatgacgc gccatttacc tgacagcgat
     4321 gtcgtctcta tttatgatgc cgcaatgcgc tataagcagg agcaaacgcc gctggcggtg
     4381 attgccggga aagagtatgg atcaggctcc agccgtgact gggcggcaaa aggtccgcgt
     4441 ctgcttggta ttcgtgttgt gattgccgaa tcgtttgaac gaattcaccg ttcgaattta
     4501 ataggcatgg gcatcctgcc gctggaattt ccacaaggtg tgacgcgtaa aacgttagag
     4561 cttaccgggg aagagaagat tgatattggt gatctgcaaa atctacaacc cggcgcgacg
     4621 gttccggtga cgcttacgcg cgcggatggt agccaggaag tcgtaccctg ccattgtcgt
     4681 atcgacaccg cgacggagtt gacctactac cagaacgacg gcattttgca ttatgtcatt
     4741 cgtaatatgt tgaagtaacg agtatttgct tgccggttat tttgcttccg gcaagcaaat
     4801 taattacaca atgcaagagg gttatttgtt cagcaaatga cccattttct cggctttggt
     4861 atcgagataa tgttcgttat tggggttgcg acctacaatc aacggtacgc gttcaataat
     4921 attaatccct gcttcggtca gaatttcgac ttttttcggg ttattggtta acaagcggac
     4981 ttcattgacg ccaaggagtt tgaacatatc agcgcaaaga gtgaagtcgc gctcatcagc
     5041 ggcgaagcct aactggtggt tagcctctac agtatcgtaa ccttgatcct gcagtgcgta
     5101 agcgcggatt ttattcagca gaccaatgtt acgaccttcc tgacggtgat acagcaaaat
     5161 accacggcct tcctcggcaa tttgcgtcaa tgccgcttcg agctggaagc cacaatcgca
     5221 gcgcaagctg aacagggcgt caccggtcag acattcggaa tggacgcgcg caagtaccgg
     5281 ggtatgcccg gaaatatcgc catagactag cgcgacatga tcgtgtccgg ttgccagttc
     5341 ttcaaatccc accatcagga aatcgcccca tggggttggc agtttggctt ctgccacacg
     5401 tttaagctgc atgaaattct ccagataatg ctggttctgt attggcttat tttgccataa
     5461 cgagaagggg ttcacctaat cacgacgcgt cgatcgttca cggaatggca caaatctgtc
     5521 aatttttcct ggaactggca ttttcagtta tgattgtggg acttatcaaa aaggagaggc
     5581 catgcgttcg attgccagac gtaccgcagt gggagctgca ctattgcttg tcatgccagt
     5641 agccgtatgg atttctggct ggcgttggca acctggagaa caaagttggt tactaaaagc
     5701 ggctttttgg gttactgaaa ctgtcaccca gccctggggc gtcattacac atttgatttt
     5761 attcggctgg tttctctggt gtctgcgttt tcgcattaag gctgccatta tgttatttgc
     5821 cattctggcg gcagcaatcc ttatgggaca aggcgttaaa tcctggatca aagacaaagt
     5881 ccaggaacca cgaccttttg ttatctggct ggaaaaaaca catcatattc cggttgatga
     5941 gttctacact ttaaagcgag cagaacgcgg aaatctggtg aaagaacagt tggctgaaga
     6001 gaaaaatatc ccacaatatt tgcgttcaca ctggcagaaa gagacggggt ttgcctttcc
     6061 ttccggtcac acgatgtttg ctgccagttg ggcactgctg gccgttggtt tgctgtggcc
     6121 gcgtcggcga acgttaacca ttgctatctt gttggtctgg gcaacgggag tcatgggaag
     6181 ccgcctgctg ctcgggatgc attggccacg cgatctggtt gtagctacgt tgatttcgtg
     6241 ggcgctggtg gcggtggcaa cgtggcttgc gcaacgaatt tgtgggccat taacaccacc
     6301 tgcggaagaa aatcgcgaaa tagcgcaacg agaacaagaa agttaacgct ggttgatttt
     6361 ccgaatttag cccttaaatc atcaacaatg cgcgtggatg ccatttcgca gacggcgcga
     6421 aaatggtact ttaaagggct attgcggtaa gttgaccata atttattcgc tctaaccaca
     6481 taacgggaag taatgtgaaa tatttactca ttttcttact ggtgttagcg atcttcgtga
     6541 tttcggtcac gttgggtgcg cagaacgatc aacaggtgac gtttaattat ctgttagcgc
     6601 aaggggagta ccgtatttcc acattgctgg cggtattgtt tgctgcgggg tttgctatcg
     6661 gttggttgat ttgcggcctg ttctggctgc gagttcgtgt ttccctggcg cgcgctgaac
     6721 gtaaaataaa gcgactggaa aaccagcttt cacccgcgac tgacgtggct gtagcgccgc
     6781 actcgtctgc ggcgaaggaa taactttcta tgctggagtt gttgtttctg cttttgcctg
     6841 tagccgctgc ctatggctgg tatatgggcc gcagaagtgc gcaacaaaac aagcaagatg
     6901 aagccaaccg cttgtcgcgt gattacgtag cgggggttaa cttcctgctt agtaatcaac
     6961 aggataaagc ggtagacctg tttctcgata tgcttaaaga ggatacaggt accgttgaag
     7021 cccaccttac gctcggaaac ctgttccgtt cgcgtggcga agttgatcgc gctattcgca
     7081 tccatcagac cctaatggaa agcgcctcgc tgacctatga acagcggctt ttggcgattc
     7141 aacaactggg gcgtgattac atggctgccg ggttatacga ccgcgcggaa gacatgttca
     7201 atcagctgac agatgaaact gacttccgca tcggcgcgct gcaacaactg ctacaaattt
     7261 accaggctac cagcgagtgg cagaaagcaa ttgatgttgc cgaacgcctg gtgaagctgg
     7321 gtaaagataa acagcgcgtc gaaattgccc atttctactg tgagttagct ctgcagcata
     7381 tggccagcga cgatctcgat cgtgccatga ctttgctaaa aaaaggtgcg gcggcagata
     7441 aaaacagcgc ccgcgtatcc atcatgatgg gacgcgtgtt tatggcgaaa gcagaatacg
     7501 ccaaagccgt cgaaagtttg caacgtgtca tatcccagga cagagaactg gtcagcgaaa
     7561 cgctggaaat gctgcaaaca tgctatcagc tgttgggtaa aactgccgaa tgggcagaat
     7621 tcctgcagcg cgcggtggaa gagaacaccg gtgccgatgc tgaattgatg cttgcggata
     7681 tcatcgaagc gcgcgacggt agtgaggctg cacaggtcta tattacgcgc cagcttcagc
     7741 gtcatccgac catgcgtgtg ttccataaat taatggatta ccacttaaat gaagcagaag
     7801 aagggcgtgc caaagagagt ctgatggtgc tgcgtgacat ggttggcgag aaggtacgta
     7861 gtaagcctcg ttatcgctgc cagaaatgtg gttttaccgc atacaccctc tactggcatt
     7921 gtccgtcttg tcgggcctgg tcaaccatta aaccaattcg cggtcttgat ggcctgtaat
     7981 ttttaaaaaa atccgacttt agttacaaca tactaattat tgatcttcta ttgtgttccg
     8041 gtaccgacgg cgcggaaaag cctgccagag gagaaatcgc aactgttaat tttttatttc
     8101 cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc atcatcaaga
     8161 aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga attctcctgt
     8221 agttgttgcc cttgattatc ataatcgcga tgccgcgctg gcctttgtcg acaagatcga
     8281 cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg ggccacagtt
     8341 tgtgcgcgaa cttcaacagc gtgattttga tatctttctt gacctgaaat tccacgatat
     8401 tcccaacact gcagcgcacg ctgtcgctgc tgcggctgac ttaggcgtgt ggatggtgaa
     8461 tgttcatgcc tccggtgggg cgcgtatgat ggccgcagcg cgtgaggcac tggttccgtt
     8521 tggcaaagat gcaccgcttt tgattgctgt gacagtgttg accagcatgg aagcctgcga
     8581 cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc tggcggcact
     8641 gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg tgcgctttaa
     8701 acaggtattc ggtcaggagt tcaaactggt tacgccgggc attcgtccgc aggggagtga
     8761 tgctggcgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg gtgttgatta
     8821 tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcagacgc tgaaagcgat
     8881 caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct ggtctactca
     8941 acggaaaccg gacgtattga tgagccaaaa gcggcccctg tacgccctaa aggcgacggt
     9001 gtggtgcgta ttcagcgtca gaccagtgga cgcaaaggta agggcgtttg cctgattacc
     9061 ggtgtcgatc tcgatgatgc cgaactgacg aaactggcag ctgaactgaa gaaaaaatgc
     9121 ggctgcggcg gagcagtaaa agatggaatt attgaaatcc agggcgataa gcgtgattta
     9181 ttaaaatcaa tgcttgaagc gaaagggatg aaggtaaaac ttgcaggcgg ctaacataaa
     9241 aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt cagttatttc
     9301 gtactgaaca gaatcaagcg tattataatt gcgagtcgat aatcgacatt tatttacgat
     9361 tatttaccga cctggtgacc aataacacca ccgacggctg caccacctaa tgtacccaac
     9421 gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacctgcgcc gattgcggtg
     9481 ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccag ggtaattgcc
     9541 agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc tgaatttatg
     9601 attcacggaa gtaagctctc tataactata atagctaaga ataagccagg tgaaattatt
     9661 ccgtgaaatc cgctcgcgcg cagggttata tcacgcaggt gataatgact tcctgttata
     9721 tcgctgataa taattttata tcttgagagt gttaataaca ggtaaatagt cttaattatc
     9781 aaccaggaat catcttagag cggatga
//
LOCUS       DAOFSV010000159         9745 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.159, whole genome shotgun sequence.
ACCESSION   DAOFSV010000159 DAOFSV010000000
VERSION     DAOFSV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.159"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            214..861
                     /gene="fucA"
                     /locus_tag="QBH27_004029"
     CDS             214..861
                     /gene="fucA"
                     /locus_tag="QBH27_004029"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDQ7016286.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            889..2037
                     /gene="fucO"
                     /locus_tag="QBH27_004030"
     CDS             889..2037
                     /gene="fucO"
                     /locus_tag="QBH27_004030"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDQ7016287.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTDEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2142..2897)
                     /gene="xni"
                     /locus_tag="QBH27_004031"
     CDS             complement(2142..2897)
                     /gene="xni"
                     /locus_tag="QBH27_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDQ7016288.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3009..4376)
                     /gene="sdaB"
                     /locus_tag="QBH27_004032"
     CDS             complement(3009..4376)
                     /gene="sdaB"
                     /locus_tag="QBH27_004032"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDQ7016289.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4434..5723)
                     /gene="sdaC"
                     /locus_tag="QBH27_004033"
     CDS             complement(4434..5723)
                     /gene="sdaC"
                     /locus_tag="QBH27_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDQ7016290.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(6280..7644)
                     /gene="ppnN"
                     /locus_tag="QBH27_004034"
                     /gene_synonym="ygdH"
     CDS             complement(6280..7644)
                     /gene="ppnN"
                     /locus_tag="QBH27_004034"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDQ7016291.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(7756..8604)
                     /gene="queF"
                     /locus_tag="QBH27_004035"
     CDS             complement(7756..8604)
                     /gene="queF"
                     /locus_tag="QBH27_004035"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDQ7016292.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQLAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8672..9217
                     /gene="syd"
                     /locus_tag="QBH27_004036"
     CDS             8672..9217
                     /gene="syd"
                     /locus_tag="QBH27_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDQ7016293.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
BASE COUNT         2546 a         2336 c         2540 g         2323 t
ORIGIN      
        1 ataaatattt ttaagagcgg tcatttttaa tggggttata aaaagagaac ttgcagttat
       61 ttactgtgat tagttgaacc aggtcacaaa atataagctt gtgaatttct ttctggttaa
      121 tcaggaagaa tatggtgagt aattaaacgg ctaattcaat agtgtgaaag gaacaacatt
      181 attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact tgctcgtcag
      241 attattgaca cttgcctgga aatgactcgc ctggggctga accagggaac agcgggtaac
      301 gtcagtgtac gttatcagga tgggatgctg attacgccta caggtattcc atatgaaaaa
      361 ctgacggagt cgcatattgt ctttattgat ggcaacggca aacatgagga aggaaaactc
      421 ccctcaagcg agtggcgttt ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg
      481 gttgttcaca atcatgccgt tcattgtacg gcagtttcca ttcttaaccg accgatcccc
      541 gcgattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc gccttatgcg
      601 acctttggaa cacgcgaact ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca
      661 actttgttac aacatcatgg gcttatcgct tgtgaggcga atctggagaa agcgttatgg
      721 ctggcgcatg aagttgaagt gctggcacaa ctttacctga cgaccctggc gattacggac
      781 ccggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt caaaacctac
      841 gggttacgga ttgaagagta atttcgtaaa gcaacaagga gaaggatgat ggctaacaga
      901 atgattctga acgaaacggc atggtttggt cgaggtgctg ttggagcttt aaccgatgaa
      961 gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc
     1021 ggcgtggtgg cgaaagtgac cgacaagatg gatgccgcag gactggcatg ggcgatttac
     1081 gacggcgtag tacctaaccc aacaattact gtcgtcaaag aagggctcga tgtattccag
     1141 aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga tacgtgtaaa
     1201 gcgattggca ttatcagcaa caacccggag tttgccgatg tgcgtagcct ggaagggctt
     1261 tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg
     1321 gcagaagtga ccattaacta cgtgatcact gacgaagaga aacggcgcaa gtttgtttgc
     1381 gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat ggatggtatg
     1441 cctccagcgc tgaaagccgc gacgggtgtc gatgcgctca ctcatgctat tgaggggtat
     1501 attacccgtg gcgcgtgggc gctaactgat gcgctgcaca ttaaagcgat tgaaatcatt
     1561 gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga aatggcgctc
     1621 gggcagtatg ttgcgggtat gggattctcg aatgttgggt tagggttggt gcatggtatg
     1681 gcgcatccac tgggcgcgtt ttacaacacg ccacacggtg ttgcgaacgc catcctgtta
     1741 ccgcatgtca tgcgctataa cgctgacttt accgatgaga agtaccgcga tatcgcgcgc
     1801 gttatgggcg tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc cgccgttgaa
     1861 gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttacgtga tgttggtgta
     1921 cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg taccggtggc
     1981 aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc ctggtaaatg
     2041 tgctgatgtg ataatgccgg atgcgacgct agccgcgtct tatccggcat ataagtgcat
     2101 taaattcaat gtgttgataa tcgtttgcat ccggcacgcc gttaccttac caaccgcaat
     2161 tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga
     2221 aacgccatct ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga
     2281 ttctcatata tcccttccag actctgaaac tcgaccagta gctgcgtggc gctttttggt
     2341 ccgatccccg caacacccgg tacctttgaa ctgctgatcc ccgcaagccc ccagtaatcg
     2401 ggcaactgct gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt
     2461 ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct
     2521 gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct
     2581 aagtcatcgg cttcgttgcc gctggcggac cagcacggga cgccgcgttg ctcaaaggcg
     2641 gcgcgtaatg caggcatctc gttgtgcaac tcttccggca ttggcggtcg acccgctttg
     2701 taatctggta aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg
     2761 accgcgtggg ttggctggct gtgcataatg agctgatcga gcgcgtgctg gcaggtttcg
     2821 acacagggcg aaccctgaac ggcatgaatg cgacgaataa gattcagtgc atcgacaata
     2881 agcaaatgaa cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg
     2941 ccagaacagg ctatggctgg atgagaaatc gggaagaggc ctcgcaaaaa gaggcctctg
     3001 gagagcgatt aatcgcaggc aacgatcttc attgccaggc cgccgcgaga ggtttcgcgg
     3061 tacttggcgt tcatatcttt acctgtttcg tacatggtct cgataacttt atcgaggcag
     3121 acgcgcggct cgctggtacg gcgcagcgcc atacgcgcgg cgttcaccgc ttttactgcc
     3181 gcaatggcgt tacgctcgat gcacggtacc tgcacctgtc cagcaaccgg gtcacacgtc
     3241 agaccgaggt tatgctccat ggcgatttcc gccgcgatgc acacctgcgc cgggcttgcg
     3301 cctaacagtt ctgccagacc agctgccgcc atagagcacg ccacgccgac ttcaccctgg
     3361 cagccgactt cggcaccaga aatcgaggcg ttcatcttat aaagagaacc aatggcgctg
     3421 gctaccagca ggtaacgcgc cagtgagtta gcgttcactt cgcggataaa cttgtcgtag
     3481 tacgccagca cggccgggat aatcccgcac gcaccgttag tcggcgcagt caccacgcga
     3541 ccgccagcag cgttctcttc gttcactgcc agtgcaaaca tgttgatcca gtcaactacc
     3601 gccatcgggt cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca
     3661 cggcgtggaa cgcgcagttt gccaggtaac acgccttcgg tagaaatacc gcgttcaata
     3721 ccgccacgca tgacttccca gacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc
     3781 agcgccagct cgtttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt
     3841 ttttgtagat cggcagcgga actgtacgga taaggaactt caaccggtgc gctatcctgc
     3901 tgaccaaaat gctcttcatc aacgataaag ccaccgccaa tagagtaata agtctggctg
     3961 taaacgactt tatcgcccgc cagcgcggta atgcgcatac cgttttcatg cagagaaagg
     4021 ttgtcggcat ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt
     4081 gccagcatca ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata
     4141 tccacggtat ccggcaggtt acccgccaga cccataatta tggcgatatc tgtgtggtgg
     4201 cctttaccgg tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta
     4261 agcaggttac gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg
     4321 gtatgagaac tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga
     4381 aatacatcgc gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg
     4441 aacagagagt agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg
     4501 ctgatgtgac cgctgtactt acgcattgcc ggtactttct gaattgcgta catcggcatc
     4561 aggaacagga tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc
     4621 gggttcaggg tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc
     4681 agcttgttga tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg
     4741 aagccttcac gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg
     4801 ataatcggag ccatccacgc gatgatcggc gcgttaaagt ggttagccag gtaagacaga
     4861 atcgagatgt tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag
     4921 ctgaagacga agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatctta
     4981 gagcatttct gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag
     5041 atgatcggag agtggttgaa cgagaacacc attaccggaa ttgccagcca cagggtcatc
     5101 cacagaccgt ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg
     5161 ttccactgcg ggatcaggta cagagccagc agcatcagta cgccaacaaa cgggaatacc
     5221 agaatactca tcgctttaac gatcatctgc tcaccgaagc gaacgatggt catcataccc
     5281 acgatcagga tcagcgacag aatcgcacgt ggcggtggcg tcatacccag ttggtgagac
     5341 atgaagcttt caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata
     5401 gcgaagaagt agagcagagt aatcagttta cctgcgccaa taccaaagtg ttcttctaca
     5461 acctcggtga tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg
     5521 tgagcaaaaa acgtcatcgg gaatgcaagg atagccatga tgatcagcgg gatcataccg
     5581 ccaacaccgg cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg
     5641 cccagcatcc acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc
     5701 gtgctggttt gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt
     5761 agccagttca gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta tcggcgatgg
     5821 cttaatcatt ttccaccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct
     5881 atcagtgatc ttgatcccaa atacaataat ctacttggaa gatagataat cacaacgctt
     5941 tactctgtaa atcaaaagta aaagagaatt taagggcaat atttgcccat taaacgacat
     6001 ctgacttctt ctggggcgcg caatatatgt aggttgaact ggaaaaatca atacttttag
     6061 ttaatcatgg cgataatttc tttttcatgg cgataaaatg tggtttatga aaatagggaa
     6121 acgtttgcgt ttcaacataa attgtatgac atattaactg tggtgatatt tgtcgcgtga
     6181 ggttggtaag aaacattcat ataacaaatg aaatgagttg atgagtaagt gaccaaaaac
     6241 gataaaaccc ggcaacaaac gtcaccgggg gaggggagat tacgtgcaga tttcgtagca
     6301 agggatgtag gctgagcctg gcaacttcat acgatgctgg gcaacaaaac cctgtagcag
     6361 gtcgtccata cgacgcataa tctctttgtc gccgttgatt ttgtaaggac caaactcttc
     6421 aatggcgcga ataccgactt cttttacatt acccgccaca ataccggaga acgcacggcg
     6481 caggtcggca gccagcactt caacgggttg atccgggtaa agcttcagat tagccatatt
     6541 ctcgtgagac ggctcaaacg gcatttgcaa atctggcgca atgcgcattg accagttaaa
     6601 gctgtaagcg tcaccagtgt cgcggcgatt ttctttcacc agcggcatcg attttttcat
     6661 ctgacgagcg acttcagcgg cgtcatcaat gatgatgcgg taatggcggc gcgcgttttc
     6721 acccagcgta tgcacgacaa actcgtccag tacgcggaag tagtcggcgc tctctttcgg
     6781 gccggtgagg atcaatggta aaacctgatc tttgttggcc gggttcatta aaattcccag
     6841 caaatacagc aactcttctg ctgtacccac accgccaggg aagataatga taccgtgagc
     6901 gatacggaca aacgcttcca gacgtttttc gatatctggc atgatgatca attcgttgac
     6961 cagcgggtta ggcggttcag cggcgataat cgacggctct gtcataccaa taaagcgact
     7021 gtctttgtaa cgctgctgcg cgtgtccgac cgcagcacct ttcatcggcg cttccatcgc
     7081 tcccggacca cagccggtgc agatattcag ttcacgcagg cccaactgat tcccgacgcg
     7141 acgggcatac aaatactcgt tttcgttaat tgagtgaccg ccccagcaga ccaccatatt
     7201 tggcgcttca ccgacatgca gcgcgcgagc gttacgcaag atggaaaaga ccaggttagt
     7261 gatgtggacg gagttgtcga gattcaggtt gggaaaacga acggtgttat gaatttgccc
     7321 ataaacgaag agaatgtcac gcagaaccgc gaacaagttg gcctgcaaag cgcgaataat
     7381 tcgcccatcg acaaaagcct cttccggggg atttatcagt tccagcttta cgccgcgttc
     7441 acgtcgcagg acgttaatat cgaaattttc aaaacgagac agcaattctt tgctgttatc
     7501 ggtcaaacta ccggagttca gtacggcaag tgaacagttg cgaaacagtt gatagaggtc
     7561 gctgctggcg gtgcgtttaa gcatatccac ttccagctgc gacaacatat ccatggagcc
     7621 aagcgggcta atatgtgtaa tcaagaaaaa ctccttatgg gacgaatatc gtcattccct
     7681 ttgattacaa tagccctgcc tgatgacctt tcacaacctt ctgcgtgaat ccagcacgca
     7741 aaattgagaa aaaatttatt gccgaaccag tcttgtggtc gatgggacaa aatcgctatt
     7801 actgcgccac gggttaatgt ccagaccgcc acgacgggta taacgtgcgt aaacgctcaa
     7861 tttctctggc tggcagaagc gtaacaggtc attaaagatg cgttccacgc actgttcgtg
     7921 gaactcgttg tgatgacgga aagagaccag gtaacgcagc agtttttctc tgtcaatttg
     7981 gcgtccacga tactgaattt ggatggaacc ccaatctggt tgatgggtga tcaggcagtt
     8041 tgatttcagc aggtggctga caagcgtctc ttccactact ttttcaccgc tggtggcatt
     8101 ctccagatag tcagtagtga attcatagtt atcgatagtg atatcctggt catcaatgca
     8161 ggtgccattg aaatgaccta tcggctggcc ttccagttca tcaagacgat ataacgccac
     8221 gctaacctta ccctgggcgc aagtgcttaa gtcgcgctct agcgtctggc gcacctcatc
     8281 ccagttatta aaacgcgtct ggttaaaact gttgagatag agcttaaaac tcttcgactc
     8341 aatcagattt acgctggtgt aatcaagttc aacatgaccg acagcgagtt gcggcaaacc
     8401 tttcgcattc agccaggaaa gttcatacag cgtccagata tccgtaccgt gaaaaggcag
     8461 gttatccgct ttcagaccca gcgggtcgcg attcaggctg cgtggaacgc cttgcagtag
     8521 gctggcgtca taggtatccc ggtaatcggt tgattttcca agtgtcaggc ccgcaagtgc
     8581 ctgatggttt gcataagaag acatgtttca ccgtcataaa ccagataaac taaacgacaa
     8641 gtgtatctgg tttaagtgaa gagagaaatt agtggacgat ttgaccgcac gagccctgaa
     8701 agattttact gcacgctact gcgatgcgtg gcatgaagag cataaaagct ggccgttaag
     8761 tgaggaactg tacggcgttc cttcaccgtg cattatttca actaccgaag atgccgtata
     8821 ctggcaaccc cagccgttta ccggggaaca aaatgtaaac gcggttgaac gcgcttttga
     8881 tattgtgata caacccacga ttcatacctt ttatacgact cagtttgccg gggatatgca
     8941 cgcgcagttt ggcgatatca aactgacatt gctgcaaacc tggagtgaag acgacttccg
     9001 tcgggtgcag gaaaatctga ttggtcatct ggtgactcag aaaaggctaa aactgccgcc
     9061 cacgctattt atcgcgacgc tggaagaaga gttagaggtc atctcggtgt gtaatctctc
     9121 cggtgaagtg tgcaaagaga cgctgggaac acgtaaacgg acgcatttag cctcaaatct
     9181 tgcggaattc cttaatcaac ttaagcctct tctgtaatcc aaatacccca tctgattgtg
     9241 agagatctct tacagactct gtaggagatc gccaggaaat aagcgaatac ttaaaaagat
     9301 aagaatcgtt attctcattt aaaaacaaca tgttgcttgt taattcttaa ctttcatatg
     9361 aaattttcct taaggcattg tctgtaagcg ccttgtaaga cttcgagaaa aagacgattc
     9421 tatcttcgtc gacagggagt cagacaacga agtgaacatc aggatgatga cacttctgaa
     9481 ggacacacca ggatggtgtt tcagggaaag gcttctggat gaagcgaaga ggatgacgca
     9541 ggacgcgtta aaggacacct ccaggatgga gaatgagaac cggtcaggat gattcggtgg
     9601 gtcaggaagg ccagggacac ttcaggatga agtatcacat cggggtggtg tgagcaggaa
     9661 gcaatagttc aggatgaacg attggccgca aggccagagg aaaagttgtc aaggatgagc
     9721 agggagcaac aaaagtagct ggaat
//
LOCUS       DAOFSV010000160         9644 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.160, whole genome shotgun sequence.
ACCESSION   DAOFSV010000160 DAOFSV010000000
VERSION     DAOFSV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.160"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1920)
                     /gene="nagE"
                     /locus_tag="QBH27_004037"
     CDS             complement(<1..1920)
                     /gene="nagE"
                     /locus_tag="QBH27_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDQ7016294.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQIPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQ"
     gene            2253..3053
                     /gene="nagB"
                     /locus_tag="QBH27_004038"
     CDS             2253..3053
                     /gene="nagB"
                     /locus_tag="QBH27_004038"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDQ7016295.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            3113..4261
                     /gene="nagA"
                     /locus_tag="QBH27_004039"
     CDS             3113..4261
                     /gene="nagA"
                     /locus_tag="QBH27_004039"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDQ7016296.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            4270..5490
                     /gene="nagC"
                     /locus_tag="QBH27_004040"
                     /gene_synonym="nagR"
     CDS             4270..5490
                     /gene="nagC"
                     /locus_tag="QBH27_004040"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDQ7016297.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            5538..6290
                     /gene="nagD"
                     /locus_tag="QBH27_004041"
     CDS             5538..6290
                     /gene="nagD"
                     /locus_tag="QBH27_004041"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDQ7016298.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            6546..8210
                     /gene="asnB"
                     /locus_tag="QBH27_004042"
     CDS             6546..8210
                     /gene="asnB"
                     /locus_tag="QBH27_004042"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDQ7016299.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(8252..8689)
                     /locus_tag="QBH27_004043"
     CDS             complement(8252..8689)
                     /locus_tag="QBH27_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016300.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGRLHIPDEQATWLVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            9023..9099
                     /locus_tag="QBH27_004044"
     tRNA            9023..9099
                     /locus_tag="QBH27_004044"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9057..9059,aa:Met,seq:cat)
     gene            9110..9194
                     /locus_tag="QBH27_004045"
     tRNA            9110..9194
                     /locus_tag="QBH27_004045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9144..9146,aa:Leu,seq:tag)
     gene            9218..9292
                     /locus_tag="QBH27_004046"
     tRNA            9218..9292
                     /locus_tag="QBH27_004046"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9250..9252,aa:Gln,seq:ttg)
     gene            9327..9401
                     /locus_tag="QBH27_004047"
     tRNA            9327..9401
                     /locus_tag="QBH27_004047"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9359..9361,aa:Gln,seq:ttg)
     gene            9417..9493
                     /locus_tag="QBH27_004048"
     tRNA            9417..9493
                     /locus_tag="QBH27_004048"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9451..9453,aa:Met,seq:cat)
     gene            9542..9616
                     /locus_tag="QBH27_004049"
     tRNA            9542..9616
                     /locus_tag="QBH27_004049"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9574..9576,aa:Gln,seq:ctg)
BASE COUNT         2424 a         2500 c         2416 g         2304 t
ORIGIN      
        1 ttgacccgcc acaacatggc cctgagcttt aatgataagg ccgctgaaat catcgatatt
       61 gctgcaaacc accgggctaa tcatcgaacg ggcgttagcg ttcaggtaat ccagatccat
      121 ttccagaatc ggttgccctg cgcttacctg cgcgccctct tccaccagac gtttaaagcc
      181 tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc
      241 ggtttccaga cagaacgcgt ggttggtgtt gaagattttc acgattgttc ctgcggctgg
      301 tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc
      361 gaatgcttcg tcaggaatct gatccagtgc cacaacatcc ccggtaatcg gcgataccag
      421 ctccgcgata gataccgcgt ttggtacagc ctgcggtttt gctacaggag cggcagttgc
      481 cggagttgct tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc
      541 atcgccgatg gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt
      601 tactacccca gaagcaccca gacgtttaca catcgcatcg ttgacgcgag ctgagtcaac
      661 cacggtaagg cgcagacgag taatacaggc gtcaatggct ttcaggttgt cagtaccgcc
      721 aaccgcagca atatagttgg ttgccagttg attgagacct tcttcagtat tgctgttagc
      781 ctcttcagta acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat
      841 gcggataacc aaactgaaca ccacgaagta gatagcgaag aagacaaccc ccatcaccag
      901 cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc
      961 ccccgcagag aaagagaagc cggcatggat acccagcaac gttgccacaa acaggctgat
     1021 accggtcagc agtgcgtgca ggaggtacag cagcggtgca aggaacatga acaggaattc
     1081 cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc
     1141 aaccatcgga cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag
     1201 gccgaacatc ataatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccggc
     1261 atagaagcgg ttgatatcac cgtggaaaac cgttcccgcc gcgttggtga attcaccaat
     1321 ctggaaccag gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt
     1381 gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc
     1441 atggatagcg tgctgtaccg gtggccagac gtaaccaaaa atggccgcca gcaccagaca
     1501 gaagaagccg gtggctatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg
     1561 cagtttaata tcggaccaac ggttataggc tgcgccacca accagaccgg taatgatacc
     1621 cgccagtaca cccatgttaa tttctgggtt gatggtcacc atcgctttgg ttaacacaaa
     1681 gtaacctacc gcacccgcca gtgccgccgc acctgcgctg tctttcgacc agctggatgc
     1741 cacgccgatg gcgaatatta atgcgaggtt atcaaaaatc gcaccgcccg cctgggcaat
     1801 aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag
     1861 caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat
     1921 cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc
     1981 aaattgtact gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga
     2041 aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc
     2101 gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc
     2161 aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc
     2221 aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg
     2281 aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccga
     2341 ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata
     2401 aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca
     2461 acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc
     2521 accgtaattt cttcgatcac gttgatattc cagcagaaaa tatcaacctt ctcaacggca
     2581 acgccccgga tatcgacgcc gaatgccgcc agtatgaaga aaaaatccgt tcttacggaa
     2641 aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg
     2701 cgtcttctct ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga
     2761 actctcgttt ctttgataac gatgttaatc aggtgccaaa atatgccctg actgtcggtg
     2821 ttggtacatt gctggatgcc gaagaagtga tgattctggt gctgggtagt cagaaagcac
     2881 tggcactgca ggccgccgtt gaaggttgcg tgaaccacat gtggaccatc agttgtctgc
     2941 aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta
     3001 agactttaag atatttcaat gaattagaag cagaaaatat caagggtctg taattgttat
     3061 ccctgccctg gctccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc
     3121 attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat
     3181 cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca
     3241 acgttcactg aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg
     3301 cggcggcgta cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat
     3361 gcagaaagcc aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag
     3421 cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa
     3481 ccaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca
     3541 taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgagaacgc
     3601 cgacgtgatt accaaagtga ctctggcacc ggaaatggtt ccagcggaag tcatcagcaa
     3661 actggcaaat gccgggattg tggtttctgc tggtcactcc aacgcgacgt tgaaagaagc
     3721 gaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta
     3781 tattaccggt cgtgaaccgg gcctggcggg cgcaatcctc gacgaagctg acatttattg
     3841 cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgtct
     3901 gaaaggcgac aaattgtgtc tggttaccga tgccaccgcg ccagcaggtg ccaacattga
     3961 acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa
     4021 cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca
     4081 ttgtggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg cacgtgcgat
     4141 tggcgttgag aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgactgcatt
     4201 cacacctgat tttaaaatca ccaggaccat cgttaacggt aacgaggtcg taactcaata
     4261 agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag
     4321 cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag
     4381 attgccgagc aaagtcagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc
     4441 gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc
     4501 tccatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcacgac
     4561 gccaccatca ccttgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg
     4621 ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt
     4681 gatagctacc agcgcaagct acgcgagctg atcgcgattt cggtgatcct gccagggctt
     4741 gtagacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg
     4801 ctggttgaag cactggaaga gcgttttaaa gtgacctgtt ttgtcggcca cgatatccgc
     4861 agtctggcgc tggcagagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg
     4921 gtgcgtgtcc atcgcggaac cggggccggg attatctcta acgggcgaat ttttatcggg
     4981 cgcaacggca acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc
     5041 cactgcggca actttggctg cctggaaact atcgccgcca atgctgccat tgaacaacgg
     5101 gtgttgaatc tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc
     5161 aaaactatct gcaaagccgc gaacaaaggc gatagcctgg cctcggaagt gattgaatat
     5221 gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa
     5281 attgttattg ctggagaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc
     5341 tgcattaata cccaggcgct gaaggcgttt cgtactaatc taccggtggt acgttctgag
     5401 ttggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt
     5461 attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc
     5521 aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga
     5581 tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag
     5641 gcctgccgct ggtgttgctg accaactatc cttcgcagac cgggcaagat ctggcgaatc
     5701 gctttgccac tgccggtgtc gatgtaccgg atagcgtgtt ttatacctcc gcaatggcga
     5761 ccgccgattt tctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac
     5821 tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct gattttgtga
     5881 ttgttggcga aacgcgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg
     5941 ctaacggtgc acgctttatc gccaccaacc cggacaccca cgggcgcggt ttttatcccg
     6001 cctgtggcgc gttgtgtgca gggatcgaga aaatctccgg gcgcaaaccg ttctatgttg
     6061 gaaagccgag tccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag
     6121 aaacggtgat tgtcggcgat aacttgcgta ccgatattct ggcgggcttc caggccggtc
     6181 tggagacgat tctggtgctt tctggtgttt cgtcgctcga cgatatcgac agtatgccgt
     6241 tccgcccaag ttggatttac ccatcggtcg ctgaaatcga cgttatctga atagcaacca
     6301 cgcctcaggg cgtggtttct tatttttacc catcattaaa atcaccccat tcaataaaac
     6361 ccgtcaaaaa ctatcgatcc atcaataaat atacccaaaa gccctcccct ttttcatcaa
     6421 tcagcaatcg ccattacgtt ttttattttt ccactgccaa atcgcttgcg caccgtgctg
     6481 ctttgcggca tttttttaac aagtaaacac aacacgcagt aaacaccagg ttaatggaga
     6541 aggttatgtg ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta
     6601 agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt
     6661 atgccagcga taacgccatt ctcgcccacg aacgtctgtc aattgttgac gttaacgcgg
     6721 gggcgcaacc tctctacaac caacaaaaaa ctcatgtgct ggcagtaaac ggtgaaatct
     6781 acaaccacca ggcattgcgc gctgaatatg gcgatcgtta ccagttccag accgggtctg
     6841 actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc
     6901 agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga tgcatatctg attggtcgcg
     6961 accatctggg gatcatccca ctgtatatgg gctatgacga acacggtcag ctgtatgtgg
     7021 cctcagaaat gaaagccctc gtgccagttt gccgcacgat taaagagttt ccggcgggga
     7081 gctatttgtg gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact
     7141 acgatgcggt gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt
     7201 ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt gctgctttct ggtggtctgg
     7261 attcctcaat tatttccgct atcaccaaga aatacgccgc ccgtcgcgtg gaagatcagg
     7321 aacgctctga agcctggtgg ccgcagttgc actcctttgc tgtaggtctg ccgggttcac
     7381 cggatctgaa agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc
     7441 acttcactgt acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt
     7501 atgatgtgac cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg
     7561 cgatgggcat taaaatggtg ctgtccggtg aaggttctga cgaagtgttt ggcggttatc
     7621 tctacttcca caaagcgccg aacgctaaag agttgcatga agagacggtg cgtaaactgc
     7681 tggccctgca tatgtatgac tgcgcgcgtg ccaacaaagc gatgtcagcc tggggcgtgg
     7741 aagcacgcgt tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccac
     7801 aggataaaat gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgaag
     7861 cgtatctgcc tgcaagcgtg gcctggcggc aaaaagagca gttctccgat ggcgtcggtt
     7921 acagttggat cgacaccctg aaagaagtgg cagcgcagca ggtttctgat caacagctgg
     7981 aaactgcccg cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc
     8041 gggagatctt tgaagaattg ttcccgcttc cgagcgccgc tgagtgcgtg ccgggcggtc
     8101 cttccgtcgc ttgttcttcc gctaaagcga ttgagtggga tgaagcgttc aagaaaatgg
     8161 acgatccgtc tggtcgcgcg gttggcgttc accagtcggc atataaataa gacgtgtaat
     8221 attgccccgg agaaatccgg ggcaatttca gttactgggg cgcttgttga ttctgatatg
     8281 cagtaaggaa atgttccggg tttgccacat cgctatttga caactcatca ctgtaggcca
     8341 ccaaccaggt cgcctgctca tccggaatat gcagtctgcc atttccctct ttcaggcgta
     8401 ataactcaaa agatttgccc ttaattacgg cggtatagga tttctcacct tcctgtaatg
     8461 ctttcacttt acagcgaaat atctgaccat cgatgtcatt atcaacatgc gatacctgaa
     8521 tgagggcttc attagaagct ttcgggccta tccgcatcat ccacaccaca gcgccttcct
     8581 gcccacgata gccaaaaaca tatttaccga tttccggttt aaccgtatcg gcgaacgcag
     8641 attgcataag taataacgca cccatcaacg ccgtcataaa acgcagcata gtattccctc
     8701 aataaatcgc ctgaagtggt agcagagatg caggcgtagc gatccctgcc ctgtcctgtt
     8761 gcaacgggcc agtatttcat agattaattt cccaccacta ttgttgaatt ccgaatttca
     8821 tcctgtatga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa acaataattg
     8881 acgaatatag cgccatgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc
     8941 ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata
     9001 aggtatcgcg aaaaaaaaag atggctacgt agctcagttg gttagagcac atcactcata
     9061 atgatggggt cacaggttcg aatcccgtcg tagccaccat cttttttttg cgggagtggc
     9121 gaaattggta gacgcaccag atttaggttc tggcgccgca aggtgtgcga gttcaagtct
     9181 cgcctcccgc accattcacc agaaagcgtt gtacggatgg ggtatcgcca agcggtaagg
     9241 caccggtttt tgataccggc attccctggt tcgaatccag gtaccccagc catattcttc
     9301 gagtaagcgg ttcaccgccc ggttattggg gtatcgccaa gcggtaaggc accggttttt
     9361 gataccggca ttccctggtt cgaatccagg taccccagcc atcgaagaaa caatctggct
     9421 acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc
     9481 gtcgtagcca ccaaattctg aatgtgtcga atatgttcgg caaattcaaa aaccaatttg
     9541 ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat
     9601 cctcgtaccc cagccaattt attcaagacg cttaccttgt aagt
//
LOCUS       DAOFSV010000161         9639 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.161, whole genome shotgun sequence.
ACCESSION   DAOFSV010000161 DAOFSV010000000
VERSION     DAOFSV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.161"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            155..1813
                     /locus_tag="QBH27_004050"
     CDS             155..1813
                     /locus_tag="QBH27_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDQ7016301.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1810..2766)
                     /locus_tag="QBH27_004051"
     CDS             complement(1810..2766)
                     /locus_tag="QBH27_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDQ7016302.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            2917..4032
                     /gene="macA"
                     /locus_tag="QBH27_004052"
     CDS             2917..4032
                     /gene="macA"
                     /locus_tag="QBH27_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDQ7016303.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4029..5975
                     /gene="macB"
                     /locus_tag="QBH27_004053"
     CDS             4029..5975
                     /gene="macB"
                     /locus_tag="QBH27_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDQ7016304.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLEWKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6048..6272)
                     /gene="cspD"
                     /locus_tag="QBH27_004054"
     CDS             complement(6048..6272)
                     /gene="cspD"
                     /locus_tag="QBH27_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDQ7016305.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6595..6915
                     /gene="clpS"
                     /locus_tag="QBH27_004055"
     CDS             6595..6915
                     /gene="clpS"
                     /locus_tag="QBH27_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDQ7016306.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6946..9222
                     /gene="clpA"
                     /locus_tag="QBH27_004056"
     CDS             6946..9222
                     /gene="clpA"
                     /locus_tag="QBH27_004056"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDQ7016307.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         2336 a         2256 c         2706 g         2341 t
ORIGIN      
        1 ttttgcaggc ttatcagcaa gagggagtat aacgcgatta ttcgctcatt tttcagacat
       61 ttgccatgct taaatgtgat gccatcacgt attagcaggc ctttcccgtt atactgccag
      121 cgtaaagaat aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt
      181 gggttttcgc ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg
      241 ggagaacgcg tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga
      301 atcagatctc taccattttg agcgcgatga tttctggttc ccgccgggag atatcaacgg
      361 gcgagaacat catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg
      421 ggttcgccgc tatcggccgc tggaagcgtg ctggacgcca tgcaccgatg gttatcaccg
      481 tattttttat cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag
      541 ttttctcgat aaagacggac atccgattga tgttgaggat attaacgatc aggctcgtca
      601 tctggtgcgt ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg
      661 taacggcacg gtgccaaatg tccccaatgt ggaagtcacc gcgcgccagc tcgatttcct
      721 cgcccgtgag ttgtcctcac atccgcaaaa tctctctgat ggacagattc gtcagggact
      781 ttccgcaatg gtgcaactgc ttgagcatta tttttctgag cagggagctg gacaggcgcg
      841 atatcgttta atgcggcggc gagccagcaa tgagcaacga agctggcgct atctggatat
      901 catcaaccgg atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct
      961 gtttgctact ttgttgcagg caaaaggcac attgcgactg gataaagacg cccgtccatt
     1021 gttgctgatc gaagatccag aaacccgttt gcaccccatt atgctttcag ttgcctggca
     1081 tctgttgaac cttctgccat tgcagcgcat cgctactacc aactcgggag agttgctttc
     1141 gttaacgccg gtagagcatg tttgccgact ggtacgtgag tcctctcgcg ttgccgcctg
     1201 gcgtctggga ccgagtggct tgagtaccga agatagccga cgcatctcct ttcacattcg
     1261 ttttaaccgt ccgtcatcgc tgtttgcccg ttgttggctg ctggtggaag gggaaacgga
     1321 aacctgggtt atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaagggat
     1381 caaggtcatt gagtttgccc agtccgggct aaagccactg gttaaatttg cccggcgaat
     1441 ggggattgaa tggcatgtac tggtcgatgg cgatgaagca gggaagaaat atgccgctac
     1501 ggtacgcagc ctgttgaata atgatcggga agccgaacga gaacatttaa cggcgttacc
     1561 ggcgctggat atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt
     1621 ggcgcaaatc ccggaaaata taccgatgaa tctgcgcaaa attatctcga aagcgatcca
     1681 tcgctcttcc aaacccgatc ttgccattga agtggcaatg gaggccggac gtcgtggtgt
     1741 ggactccgta ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg
     1801 tcgggccgac taactacaaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc
     1861 atagcgccga cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc
     1921 tatctgtgct ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca
     1981 gaaagcgttg taatcggcat ggatcttgcc ttctttatcg cgataacgca ggctgcggta
     2041 aatatgcgtt tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag
     2101 acaggccgct tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt
     2161 ctggatttcc tggtgtggaa tttcccattt tgcgccttgc agtccgccaa taaacatcgt
     2221 ccttttcccc tgatattcac acagggtaaa agtgagctct gccagaggaa taccttcgct
     2281 gttgcggaac aggatggtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc
     2341 aagcgtgaac tgctcgccgt ttttgccttc cagcttcgcc agttgcagcc cgggggtatt
     2401 caaatataag ctgaattctt ccgccgacat acacccgcgg agtaacgcat aatggtaacg
     2461 taacgcctcc agcaattgct tacggctaag attcgccgca aggtaagggc gatgtagacg
     2521 tacaggcagt cgcggctggc gcgttaacaa cacattgaga ttaggccagt gggaaagttc
     2581 gttcatccac tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg
     2641 aaaactacgg cgatgccaga atttacccgg ccgacactgt cctcgtgcca gactaagaaa
     2701 aagtgacagg ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat
     2761 attcatgaaa tcaatggtta tacatgacgt cgatttaacc attgcgtatc ttaaccaaac
     2821 atcaatagtg tgattattaa cgtaaatttt agggttttgt tgatatttcg ttgaagttaa
     2881 tgacccggat tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag
     2941 aagcgttacg ttattgcgat ggtgatagtc atcgccgggc tgattacgtt atggagaatt
     3001 cttaacgcac ccgtgccgac ttatcagacg ctgattgtgc gccccggtga tttacagcaa
     3061 agcgtgctgg cgaccggaaa gctggacgcg ctgcgtaagg ttgacgtggg cgcgcaggtc
     3121 agcggtcagt tgaaaacgct gtcggtggcg attggcgata aagtaaaaaa agaccagctt
     3181 ttaggggtta ttgatcctga acaggctgaa aaccagatca aggaggtgga agcaacgctg
     3241 atggagctac gcgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg
     3301 tattcccgtc agcaacgtct tgcacaaacg caagcggttt cacagcagga tctcgataac
     3361 gccgcgacgg agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag
     3421 cgcaatcagg cctctctcga tacggctaaa accaatctcg attacacccg catcgttgcc
     3481 ccgatggccg gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca
     3541 caacaagcac cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag
     3601 gtttctgaag cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtgctt
     3661 ggcgatccac tgacgcgcta cgaggggcaa atcaaggatg tgctaccgac gccggaaaag
     3721 attaacgacg ctattttcta ttacgcccgt tttgaagtac ccaaccccaa tggtttactg
     3781 cggctggata tgaccgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg
     3841 atccctctgt cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg
     3901 cgtaatggtg aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt
     3961 gagattgtca aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga
     4021 gctgcgcaat gacgcctttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg
     4081 atgagcaggt tgaggtgctg aagggcatca gcctcgatat ttatgccggt gagatggtag
     4141 cgattgtcgg tgcttcgggt tctggtaaat cgaccctgat gaatattctc ggctgcctgg
     4201 ataaggccac cagcggcacc tatcgcgtcg ccggtcagga tgttgccacg ctggacgccg
     4261 atgcgctggc gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttgc
     4321 tttcgcattt aaccgccgag cagaacgttg aagtacccgc cgtctatgcc ggtcttgagt
     4381 ggaaacagcg gctgcttcgc gcccaggagt tactgcaacg actggggctg gaagaccgta
     4441 cagagtatta cccggcacag ctttcgggtg gccagcaaca gcgcgtcagt atcgcgcggg
     4501 cattgatgaa cggcggtcag gtaattcttg ccgatgaacc gaccggcgca ctggacagcc
     4561 attctggcga agaggtgatg gcgatcctgc atcagctgcg cgatcgcggg catacggtga
     4621 ttatcgtcac acacgatccg caggttgctg ctcaggccga gcgggtgatc gaaattcgcg
     4681 acggcgaaat tgtgcgcaat cctcccgcca ttgaaaaagt gaatgctact ggcggaacgg
     4741 aacctgttgt caacacggcg tctggctggc ggcagtttgt cagcggtttt aacgaggcgc
     4801 tgacgatggc atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg
     4861 ggattattat cggtattgcg tcggtggttt ccattgtcgt ggtgggcgac gccgccaaac
     4921 aaatggtgct ggcggatatt cgttctattg gtacgaatac tattgatgtc tatcccggga
     4981 aagattttgg cgatgacgat ccgcaatatc agcaggcgct gaagtacgac gacttaatcg
     5041 ccatccaaaa acaaccgtgg gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc
     5101 tgcgttataa caatgttgat gtggctgcca gtgccaatgg cgtgagcggc gattatttta
     5161 atgtctatgg catgactttc agtgaaggga acacctttaa tcaggagcag ctgaacggtc
     5221 gtgcgcaggt cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag
     5281 atgtggttgg cgaggtgatt ctggttggca atatgcccgc cagagtcatt ggtgtggcgg
     5341 aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg tgtctggctg ccatacagca
     5401 cgatgtccgg gcgagttatg ggtcagtcgt ggctgaactc cataactgtc agggtgaaag
     5461 aaggctttga cagcgctgag gcggaacagc aactcacgcg tttactttca ctgcgccacg
     5521 gtaagaagga tttctttacc tggaacatgg atggcgtctt gaaaaccgtt gaaaagacca
     5581 cacgtacttt acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta
     5641 ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc
     5701 gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca gtttctgatc gaagccgtac
     5761 tggtttgcct ggttggtggc gctttgggaa taacactgtc actgttaatt gctttcacct
     5821 tgcagctttt cttacccggc tgggagattg gtttttcacc gttagcgctg ctgctggcgt
     5881 ttctctgctc gacggtcacc gggattttat ttggctggtt acccgcacga aatgcggcac
     5941 gactggatcc agtagatgct ctggcacggg agtaattttt gagataaaaa tgccagccga
     6001 tcgggctggc attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc
     6061 gcttctactt cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca
     6121 tcaaactgaa cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa
     6181 taatgagcga aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca
     6241 ttgttgaacc acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac
     6301 aagtaagatg gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag
     6361 aaatacaatt tagagaatta gggcgagccg tcaagcattt gacaggggcc agggggcagg
     6421 tatgaatcaa aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga
     6481 tgccaacgat agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc
     6541 ttgtcagcgg cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt
     6601 aaaacgaacg actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta
     6661 aaaccgccat ctatgtataa agtgatatta gtcaatgatg attatactcc gatggagttt
     6721 gttattgacg tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg
     6781 ctcgctgttc actaccaggg gaaggccatt tgcggagtct ttaccgctga ggttgcagaa
     6841 accaaagtgg cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg
     6901 ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa
     6961 ctggaactca gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg
     7021 accgtcgagc acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa
     7081 gcgtgttctg tagatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc
     7141 acacccgttc tgcctgccag tgaagaggag cgcgacacac agccgacgtt gagttttcag
     7201 cgtgtactgc aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggttaccggt
     7261 gcaaacgttc tggtcgctat ctttagcgaa caggagtcgc aggcggcata tctgttgcgc
     7321 aaacatgaag tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac
     7381 gagccgacac agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg
     7441 gaggaacgta tggagaattt cacgacgaac ctgaatcagc ttgcgcgagt gggcggaatc
     7501 gacccactaa ttggtcgtga gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc
     7561 cgtaaaaaca acccgctgct ggtgggggag tctggtgtcg gtaaaaccgc gattgcggag
     7621 gggcttgcat ggcgaattgt tcagggcgat gtgccggaag tgatggctga ctgtacgatt
     7681 tactctctcg atatcggttc cctgttagcg ggcacaaaat atcgcggcga ctttgaaaaa
     7741 cgttttaaag cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat
     7801 gagatccaca ccattatcgg tgcgggtgca gcgtctggtg gccaggtcga tgcggctaac
     7861 ctgattaaac cgttgctctc cagcggtaaa atccgcgtaa ttggttcgac aacctatcag
     7921 gagttcagca acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat
     7981 attactgaac cgtcaatcga agaaactgtt caaatcatca atggcctgaa accgaagtat
     8041 gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc gtgcggcggt agagctggcg
     8101 gtgaaataca ttaacgatcg tcatctgccg gataaagcga ttgatgttat cgacgaagcg
     8161 ggcgctcgcg cacgtctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat
     8221 attgaatccg tggtggcccg tattgcacgc attccagaga agagtgtttc tcagagtgac
     8281 cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa
     8341 gccattgagg cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat
     8401 aaaccggtcg gttcgttcct gtttgccggc cctactgggg tcgggaaaac agaggtgacg
     8461 gtacagcttt cgaaagcgtt gggcattgag cttctgcgct ttgatatgtc cgagtatatg
     8521 gaacgccata ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag
     8581 ggcggtttgc tgactgatgc ggtcatcaag catccacatg cggtgctgct gctggacgaa
     8641 atcgagaaag cgcacccgga cgtgttcaat attctgttgc aggtgatgga taacggtacg
     8701 ctgaccgata acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccact
     8761 aatgccgggg tacgggaaac tgagcgcaaa tccattggtc ttatccacca ggataacagc
     8821 accgatgcga tggaagagat caagaagatc tttacaccgg aattccgtaa ccgtctcgac
     8881 aacattatct ggttcgatca tctgtcaacc gacgtgatcc atcaggtggt ggataaattc
     8941 atcgtcgagt tgcaggttca gttggatcag aaaggtgttt ctctggaagt gagccaggaa
     9001 gcgcgtaact ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg
     9061 cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg
     9121 gtggacggcg gtcaggtcac cgtcgcgctg gataaagaga aaaatgagct gacttacgga
     9181 ttccagagtg cacaaaagca caaggcggaa gcagcgcatt aatctgattg tctggtaggt
     9241 tggtgaagtc cgtaatctcg tcaggggtta cggacttttt atttatgggg ggaggaggtt
     9301 cagacccttt ttttgatgat gatggtaagt tattgataat tagtgctgcg ggtaggtaag
     9361 gataaaaaag ggtggcagca ggagattgag atggttttgc tttattaaca acgggctaaa
     9421 cgtgtagtat ttgagttcac tgccgtacag gcagcttaga aattgccgcg gatcctgtct
     9481 gccaataatg acaagttcac tgccgtacag gcagataaaa tgcgaaaaaa aagcccgtac
     9541 tttcgtacga gctcttcttt aaatatggcg gtgagggggg gattcgaacc cccgatacgt
     9601 tgccgtatac acactttcca ggcgtgctcc ttcagccac
//
LOCUS       DAOFSV010000162         9373 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.162, whole genome shotgun sequence.
ACCESSION   DAOFSV010000162 DAOFSV010000000
VERSION     DAOFSV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.162"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(12..530)
                     /gene="fabA"
                     /locus_tag="QBH27_004057"
     CDS             complement(12..530)
                     /gene="fabA"
                     /locus_tag="QBH27_004057"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDQ7016308.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(599..2359)
                     /locus_tag="QBH27_004058"
     CDS             complement(599..2359)
                     /locus_tag="QBH27_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDQ7016309.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            2545..2997
                     /gene="matP"
                     /locus_tag="QBH27_004059"
     CDS             2545..2997
                     /gene="matP"
                     /locus_tag="QBH27_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDQ7016310.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(3073..4125)
                     /gene="ompA"
                     /locus_tag="QBH27_004060"
     CDS             complement(3073..4125)
                     /gene="ompA"
                     /locus_tag="QBH27_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDQ7016311.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(4481..4990)
                     /gene="sulA"
                     /locus_tag="QBH27_004061"
     CDS             complement(4481..4990)
                     /gene="sulA"
                     /locus_tag="QBH27_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDQ7016312.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            5209..5838
                     /gene="sxy"
                     /locus_tag="QBH27_004062"
     CDS             5209..5838
                     /gene="sxy"
                     /locus_tag="QBH27_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDQ7016313.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(5801..7963)
                     /gene="yccS"
                     /locus_tag="QBH27_004063"
     CDS             complement(5801..7963)
                     /gene="yccS"
                     /locus_tag="QBH27_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDQ7016314.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(7973..8419)
                     /locus_tag="QBH27_004064"
     CDS             complement(7973..8419)
                     /locus_tag="QBH27_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDQ7016315.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            8542..>9373
                     /locus_tag="QBH27_004065"
     CDS             8542..>9373
                     /locus_tag="QBH27_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDQ7016316.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     A"
BASE COUNT         2451 a         2327 c         2389 g         2206 t
ORIGIN      
        1 tctgattatt atcagaaggc agacgtatcc tggaacagac cgactttcag gtcgttggcg
       61 gtatagatca aacgaccatc aaccagcact tcgccatcag ccaggcccat aatcagacga
      121 cggttaacta tgcgtttaaa gtgaatacga taggtcactt ttttcgccgt cggcagcacc
      181 tgacctgtga atttcacttc acctacgccc aacgcgcgac ctttaccttc gccgcccagc
      241 cagccgaggt agaaccctac cagctgccac attgcgtcca ggcccaggca tcccggcata
      301 accggatcac caatgaagtg gcatccgaag aaccacagat ccggattgat atccagctct
      361 gcttcaacat accctttgtc gaagttacca cccgtttcgg tcattttgac cacacggtcc
      421 atcatcagca tgtttggtgc tggcaattgc gggcctttag cgccaaacag ttcaccgcga
      481 ccagaggcaa gaaggtcttc ttttgtatag gattcgcgtt tatctaccat gttctctgta
      541 agccttattt tattgaagca cgcaggatag ctaacacgtg tacgctgaac aagtccgatc
      601 agttcggaat aaaccagttc agccaacgta atggccatgg aaaacggtga cgtccttcct
      661 gttgcgatgc ttgtgcgata cgttcctgga tggtttgcat cagcgtcgtc tggccttcgc
      721 catcccacac cagatttaat aataacggca gtgcgtcagt cacatcgtct actgcccaga
      781 tggtgaattt gccttcttct accgctttca ccagttcact gtgaagactt aaatggcgaa
      841 cgttagcagt ggggataatg acaccttgtt tccccgttaa ctcacgttgc tggcaaatag
      901 caaagaagcc ttcgattttc tcatttaaac caccgactgg ctgggcgcga ccgaactgat
      961 cgactgaacc tgtgatagcg atactctgat tcaccggcac atcggcgagg gcgctgatca
     1021 gagcgcagag ttcagccatc gaggcactat cgccatccac ttcactgtat gactgctcaa
     1081 atgtcagcga tgctgagaag gggatctgtt gctcaagctg tagttccgac atcaggaacg
     1141 cttgcatgat catcatccct ttcgcatgga tattgccgcc aagctccgct ttgcgttcga
     1201 tgtcggtgaa ttcaccatcg ccaatatgca caacgcagct aatgcgagaa ggttcgccaa
     1261 aagcgcgtgg atgacccgga aattcaatga ccgaaagggc gttaatttgc ccgatgcgtt
     1321 cgccttcggt ttcaatcagg atttgctcct gaaggatctc atcctgcatg cgttcagcga
     1381 ggaaaccttc gcgccattca cgctgctgta gcattaagtt tagctgctcg ccggagaagg
     1441 tgtcgccatc acacagggag gcgacctctt gacactggcg gaggatccac tgcgggctaa
     1501 gcggaagtgt ttcttgttca ccagtgtagc gtgctgcttc gcggataagt accggccagg
     1561 catccgctcc cggtgctggt aagtgattat gtctggcggt aaatgtcacc cagcgacacc
     1621 actggcttac tgactccgca tcgacaatct gcagagtatc ttcaaattcg ctataaatag
     1681 cctgctctga aagctctggc tccatctcct ggaaatcagc caatgattcg cgttcgccta
     1741 ccagaatgac tttcagcttc aatggcatcg aaggcacaga gacggggaga gggcgcgact
     1801 catcaaacgc aacccagtca aaacgctcgc ggttaacgat atttttcagc cgcatccaca
     1861 gcagaggttg cgccagcagt gtacgcaaag agatgatgag aataccgcca tttgcctgat
     1921 gcaccagacc aggctgcagg gtaatatcgc cattaaactg acgcaggcag ccaaagagtt
     1981 gctctgcttc tacccagtcg gcagcgacaa cttgcgttaa agtcgcaaaa ttatcatctg
     2041 cactcactgc gtgacgtaag cggatggtgt ggccggaaac ctcatagtga ccgcccacca
     2101 gttggcctgc atcgctttgt agcgtacgcg cggcatcagc aataagattc agatactcag
     2161 actcttccgg ggccttcgcc agcataaaag aggaggatgc tcgcgtatgc agcaactgct
     2221 ccagcgcaaa ttgcagtcgc ggttgagtat cactgaataa aggatcgttt tcgtcaatca
     2281 aatgtggctg agcaaatatt tcctgatagc tatcggtatc aggaaccagg tcacgccatg
     2341 caagtttcgt aatggtcaaa gttgatgttt tttagtctgt tgtcaaagcc gcgattatac
     2401 cgtaaccggc actgcagcac acgtagaaag caacgacaat acgcctggca tgggcgttaa
     2461 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
     2521 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
     2581 tggaaatgga agtatctggt gaagaagcac cgcgaagggg agttaatcac ccgttacata
     2641 gaagccagtg ccgcccagga agccgtcgat gagttgctct cgctggaaaa tgaacccgtg
     2701 ctggtaaatg gttggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
     2761 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
     2821 tcgatcgatc tggaatttat cgtctggcaa cgtctggctg gccttgcgca gcgtcgcggt
     2881 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
     2941 gcgaataaaa tgtcttcttt gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
     3001 tcaaaagtca gttcaattta cgcaaggcga aaaaaaaccc cgcagcagcg gggtttttta
     3061 ccagacgaga acttaagcct gcggctgagt tacaacgtct ttgatgcctt taacttcgat
     3121 ctctacgcga cgatccggag ccaggcaatc gatcagtgca gcacgctgtt tcacgttgtc
     3181 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga ttttgtctgc
     3241 cgggatacct ttagagatca ggtaatcaac aacggactga gcacgacgct cggacagagc
     3301 ctggttataa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
     3361 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
     3421 tttcagggtt gctttgttga aggtgaacag aacgtcagac ttcagagtga agtgcttggt
     3481 ctgtacttcc ggtgccggag ctggagccgg agcaactact ggagctgctt cgccctgacc
     3541 gaaacggtag gaaacaccca ggctcagcat gccgttgtcc ggacgagtgc cgatggtgtg
     3601 tgcatcaccg atgttgttgg tccactggta ttccagacgg gtagcgattt cgggagtgat
     3661 cgcatactca acaccgccag cgaagaccgg agaaacgccg gtgtcgtggt ctttaaagga
     3721 tgcgccacca ggtacgttag ccttggtgtc tgcacgccat accataccac ccagacgagt
     3781 gtagatgtcc agatcgtcag tgattgggta acccagttta gcggtcagct gaacgccctg
     3841 agctttgtat gcgccgttga tgttgtcgcc tttgtacggc atacgaccta accagtcgta
     3901 acccatttca aagccaacat acgggttaac ctggtaacca ccaaaagcac ctgcacccag
     3961 ttggttttcg tgggtcggac cattgttagg aataaaacca gtgtcatggt actgggacca
     4021 gcccagttta gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc
     4081 gaaaccagcc agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt
     4141 atcatccaaa atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg
     4201 ggatctgctc aatattaact ctaccgatat cttcggctta tgccgagcac ccctggcgat
     4261 gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac tccgtcaggc atatgaaaaa
     4321 aaagtcttgt ataaggtacg tttaatcttt tttgtcagcg acaatttaca gaagaaaatc
     4381 gcggaaaccg cttcagacaa gcctccgcaa ggaaaattag tcacgactga aagcattggc
     4441 tgggcgataa aaaaagttcc aggattaatc ctaaatttac ttaatgatac aaattagagt
     4501 gaatttttag cccggaaagt tgtctcgttg cgtgagagga tgcgcttacc ggacgcataa
     4561 taaaccccat agcgttacct tcatttgccg catcaacaag ttcagcatgc tcttctgcag
     4621 tcaaatcatc tgccaaccaa ccgatcacca cgctgtaatt gcccgtgcgt aaagcgcgaa
     4681 ccattgactc cacggtgtgg caaggggaga gctggctaat ctgcattact ttcgttaagg
     4741 gtagcccaga tgcctgaacc cattcgcgac tcagtttttg ttgcggtgtt aaccagagtt
     4801 gccagcgtga ttgctgaccg agttgctgta acaatggcaa cagtagaagt tgcgtcatca
     4861 tgggctgatc ttcgcgatag acaacttcac tgataagccc ggctgtagtg ttttccgtag
     4921 agacacgcgc aattttactt gctgcggatg agaacgacga agaacgatgt gcatagcctg
     4981 aagtgtacat aatcaatcca gcccctgtga gttactgtat gaatgtacag tacatccagt
     5041 gacgacaaag atcaacccta ttttcggaaa gagcctcgca aattttgtcg ttggtggcgg
     5101 gaaaacataa attaatcttg ccccttaaga ataagttgcc tattttcgta gttaacggat
     5161 ccgttaatgt gaatcattct tttatgttat gattttaaaa ggaattttat gaaaagcctc
     5221 tcctataagc ggatctataa atcacaagaa tacctggcaa cgttgggcac aattgaatac
     5281 cgatcattgt ttggtagtta cagcctgacc gttgacgaca cggtgtttgc gatggtttct
     5341 gatggtgagt tgtatcttcg ggcttgtgag caaagtgcac agtactgtgt aaaacatccg
     5401 cctgtctggc tgacatataa aaagtgtggc cgatccgtta ccctcaatta ctatcgagtt
     5461 gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc tgtcgaaata ttctctcgat
     5521 gcagcgctga aagagaaaag cacgcgcaat acccgggaaa gactgaaaga tttgcccaat
     5581 atgtcttttc atctggaagc gattctcggg gaggtgggga ttaaggatgt acgggcgtta
     5641 cgtatacttg gggcaaaaat gtgttggttg cgactgcggc agcaaaactg tctggtgaca
     5701 gaaaagattc tgtttatgct tgaaggggcc attatcggca ttcatgaagc tgcgctccca
     5761 gtggcacgcc gccaggagct tgcagaatgg gctgactctc ttacgccgaa acaggagttt
     5821 cctgcggaac ttgagtaatc tggcgttgca gacgaccaat ctctggcagt aatgcaatca
     5881 ataacccaac ttgttgtacg accagaggtt ctttgctgtc tgcccgtggt tcaagttgct
     5941 gcatccgctg tttcaggcca gctaatgcct gattgacgcg ttcttcatca gcaggttgat
     6001 gatgtagcgc gtcatcaaca tagcaaacgg cgtcatcaag aaacgccaga atttcaggat
     6061 tagttaactg ctcccggtga gcaccgaggg ctgagatata gctggtaaac gtatggttaa
     6121 ggcacagcaa ccgaaacgcg gcttcgcgaa tttgcggggt aacgttcggt tcgctggaca
     6181 tatttgatac caccgacgcc agttcagcat cacggttgtg tgcatcgcgg cgggcaatac
     6241 gatacgccag acggttatca cgcccctgat ggtattgttc cagtatggca tcgagatacc
     6301 ggcagttggc ctctgtggcg cgttcgagca ttcgcggcag attgcgaaac ttccagtcag
     6361 gccagatgta gctcactgcc gcccacgcaa tggcacaacc aatcagcgta tcgattacgc
     6421 gaggtaacgc cacttcaaaa ccttcaccca gcaagttgaa acacagtagc accaaaagtg
     6481 tgatgaacat cgttgcatgg gcgtattgca cgttacggaa ggcaaaaaag agcacgccgg
     6541 taataaccag cagcaccagc tgcccttcca atgatggcac aaaccacagc acaggaatac
     6601 caattgcgat acctaccagc gtaccaataa tccttaactt caggcggtgg cgcgtggcgt
     6661 tatagtttgg ctggcagaca aacaaacttg tcagcaagat ccaatacccg tgatgcattc
     6721 cggttatctg aatgatggcg tagccgaagc acaacaccag cgacattctt accgcatgac
     6781 ggaagagggc ggattccggc gtgaagtgac ggctaagacg cagccagata tcactcaatc
     6841 cgtgcgggct gtcatcagcg agctcatttt cgtcattatt atggggcagt gcctgggcct
     6901 gttctgattc aattgttgcc agttgggcat caatggcacg taaattgttc agtaaaaatc
     6961 ccagtgtttt gagtaaatca gcaggcgcgc cgttatcgcg catccgctcc agcgcagcat
     7021 caatatgcgt aaaagcgcgc tcaaaatgcg gatcatgttg ataaggctga cgcagcaaaa
     7081 tacagcgtga cagttgctgg cacgcctggc cctgcatcga catcagccgc tgaaaacgga
     7141 acagcacgtc gctgtggcga aaatgttcac gcaatgtttg atattgaata tgagaagagc
     7201 tggcacgctc gtgaatatcc tgcgcgacaa agtaataatg cagtgtgcga cgcgttcccc
     7261 gttgaccacg atcgccacgt aagcgggtca gcagcgagag tttcgtctga ttcaatgtcg
     7321 ccatcagctg accgttggcg agagccaaat cgtacagcgg tgcctggctt tcatcttcaa
     7381 tatcaggatc aaacatgcgc gacttgagct caagataacg cgccagttgt tcatagcaac
     7441 gcgccaggtt gtcctgcagc gggcggaccg ggaacagcag atgaccaata agtgtcagga
     7501 cgttgtacca gacggcaccg gccagaagat acatcggctg ctgataccag tgctcataca
     7561 gtgatgttcc caacatagtg taaatggcga tcagcaatgc accgaaggca attgttgcat
     7621 agcgttgacc cagaccgccg agcaaaatga agccgctggt agagagcgtt aagccaatcg
     7681 caaatagcca gggccaggga aacagcaatt ctactgaggc cgaggcgata aaaaagcaga
     7741 acagcgtaat gatgaggtta cgcaaacgtc ccgccagtcg gtcatcgaga tcggtcagcg
     7801 ccgctgccac catcccaagc gtcagcggaa tcgtcagttt tacatcaccc agccaccacg
     7861 gaaacgctgt ggttccacaa agcgcaataa aaatacgcgc gtaatacagc caggcgctgt
     7921 tccaggtata gcgtttgagc aaaggactta gcataaaggc catattagtg gattattcaa
     7981 aacgacgacg tgcattggct tcgcgcgcag cttgcgctgt ttctaccgat accacgcgac
     8041 gaccgaccgg ccatagtgca atagcggcaa ttttaaagtt cgcaataccg acaggaatgc
     8101 caatgatgga aatacattgt gcgatgcccg ttgcaatgtg catcaggcat aaccaccagc
     8161 caaagaaaat cagccagaaa atattcaata ccgtaccgcc agtattcagc agcacatttt
     8221 tgccagccgg gttcagttca tcgacatgaa tagcttcatt gccataaggc accagagaca
     8281 gtttagtgat ctcccagcag gatcgtgtca gtggtaaggt aaaaatcagc acaatactga
     8341 ccagagtcgc caacagccag cccagagtgg tggcaaatcc gccaagcaca aagttcagaa
     8401 tgttcaaaac ggtacgcata aaacctcgct ttactgtggt tttcagtaat gggcggcaat
     8461 tgtaacgttt ttttgggctg gagcacgttt tctctgacgg ttacactgat aagaaataat
     8521 ttcgtgtgga tctacagagt catggaactg aaagcgacaa cgcttggaaa acgtctggca
     8581 cagcaccctt acgatcgggc ggtgatcctc aatgccggga ttaaagtctc cggcgatcgc
     8641 cacgaatacc ttattccttt caatcaatta ctggcgattc actgtaagcg cggtctggta
     8701 tggggcgaac tggagtttgt actgccggac gaaaaagtgg tgcgtctgca cggcaccgaa
     8761 tggggcgaga cgcagcgttt ttaccatcat cttgatgctc actggcggcg gtggagtggc
     8821 gagatgagcg aaattgcgtc tggcgtttta cgtcagcaac tggatttgat tgccacgcgc
     8881 accggagaaa ataaatggct gacgcgtgag caaacctctg gtatgcagca acaaatccgc
     8941 caggctttgt cggcgttacc attgccggtt aaccgactgg aagaattcga taactgccgt
     9001 gaggcgtggc gtaaatgtca ggcctggttg aaagacattg aaggcgctcg gttgcagcat
     9061 aatcaggcgt ataccgaagc catgcttacc gagtatgcgg attttttccg ccaggtcgag
     9121 tcttcaccgc tgaatccggc gcaggcccgg gcagtcgtta atggcgagca ttctttgtta
     9181 gtgctggctg gtgcaggaag cggaaaaacg tcggtgctgg tggcccgtgc aggctggttg
     9241 ctggcgcgtg gtgaagcgtc ccctgagcaa attttattgc tggcgtttgg tcgcaaagcc
     9301 gctgaagaga tggacgagcg tattcgagaa cgactacata ccgaagacat taccgcacgc
     9361 acatttcatg cgc
//
LOCUS       DAOFSV010000163         9274 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.163, whole genome shotgun sequence.
ACCESSION   DAOFSV010000163 DAOFSV010000000
VERSION     DAOFSV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.163"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            463..891
                     /gene="uspC"
                     /locus_tag="QBH27_004066"
     CDS             463..891
                     /gene="uspC"
                     /locus_tag="QBH27_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDQ7016317.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGG"
     gene            complement(898..2322)
                     /gene="otsA"
                     /locus_tag="QBH27_004067"
     CDS             complement(898..2322)
                     /gene="otsA"
                     /locus_tag="QBH27_004067"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDQ7016318.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2297..3097)
                     /gene="otsB"
                     /locus_tag="QBH27_004068"
     CDS             complement(2297..3097)
                     /gene="otsB"
                     /locus_tag="QBH27_004068"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDQ7016319.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAITAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3264..4250)
                     /gene="araH"
                     /locus_tag="QBH27_004069"
     CDS             complement(3264..4250)
                     /gene="araH"
                     /locus_tag="QBH27_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDQ7016320.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4265..5779)
                     /gene="araG"
                     /locus_tag="QBH27_004070"
     CDS             complement(4265..5779)
                     /gene="araG"
                     /locus_tag="QBH27_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDQ7016321.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTNMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5849..6838)
                     /gene="araF"
                     /locus_tag="QBH27_004071"
     CDS             complement(5849..6838)
                     /gene="araF"
                     /locus_tag="QBH27_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDQ7016322.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7633..8136
                     /locus_tag="QBH27_004072"
     CDS             7633..8136
                     /locus_tag="QBH27_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDQ7016323.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8214..8465)
                     /locus_tag="QBH27_004073"
     CDS             complement(8214..8465)
                     /locus_tag="QBH27_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDQ7016324.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8580..8666)
                     /gene="azuC"
                     /locus_tag="QBH27_004074"
     CDS             complement(8580..8666)
                     /gene="azuC"
                     /locus_tag="QBH27_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDQ7016325.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8930..9253
                     /locus_tag="QBH27_004075"
     CDS             8930..9253
                     /locus_tag="QBH27_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDQ7016326.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2438 a         2333 c         2093 g         2410 t
ORIGIN      
        1 ttttgttttg ggtgtatttc aatctgttaa aaaatttttc gctacactgg caagcaaaaa
       61 tgaaacagga ataatcgaaa tgggatgttg cgtacagtta aaataactca ccgtaaataa
      121 tcatctgcta taaataatcg ctttaatgca ataccaggta agccattttt aaacaaacac
      181 ttaccgcaca acaaactaat taactacaaa gaaaggcaac aatcaaccta atgttaataa
      241 aatgctttac atatattaac cacttgttca atatgggaaa tcagtacatt attttcttat
      301 aaatacagtt gaaatcatat gataaccaag agcaattgat taattgtatt tactaatgac
      361 cagactaata tttacctgtt taactgagtt gggattgcgt cgtttctcca ttaggagtaa
      421 agctttaatg tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt
      481 cttgtcgccg ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc
      541 gccaggccag taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac
      601 aatcaattag ctgcgccgat gcttgaagat ttacgcaacg tgatgcagga agaaacgcaa
      661 agctttcttg ataagttaat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc
      721 tacggtgagt taagcgaaca tattctggaa gtttgtcgca aacatcattt cgatttggtg
      781 atttgcggta atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg
      841 attacctcaa gtgaggtcga tgtgctgtta gttccactta cgggaggtta accgcaccta
      901 cgcaagctta ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac
      961 tatctgcttt aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat
     1021 aacgtccagc atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg
     1081 atccagcgca gctgcaactt cgtcacgatc gtagggatta acaattaacg ccgacgttaa
     1141 ctcgtttgcc gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg
     1201 agcggcaaca tactcttttg ccaccaggtt catcccgtca cgcagtggcg tcactaagcc
     1261 cacgtcagag tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata
     1321 ataaagcggc gtccagccta attgcccata tttaccatta attcgtccgg cttcattttc
     1381 gagctgatga cgaatatcct gataggcttg cacatcacca cgagacgttg gcgcgatctg
     1441 ggtataacga attttaccgt gatgttgcgg atatttttcc agtaacgctt cataggcgag
     1501 aaaacgctcc ggcaaacctt tggaataatc cagtcgttcg acagaaaaga tattttgtac
     1561 gtttttcagt tctgctttga gttgcgccag ttttggcggc agtggaccgg cagcctgttt
     1621 cgctatttct ttcggctcaa tgccgatcgg gtaaacttcc gttcgaaacg ctttgccgca
     1681 ggccgtatgg ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa ggcaatccag
     1741 gaacgccaga cgatcgttct ccgtctggaa acccagcaaa tcataatcac aaagctgttc
     1801 aagcaaggtg tcatatgtcg gcagcgcgtt gaagatttcc ggcgtcggga aaggaatatg
     1861 cagaaagaaa ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaagggcaa
     1921 caggtgataa tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc
     1981 tgccagcaac gcatttacgc gtagatagcc atcccaggca ggacgctgaa attgcaccag
     2041 atcgagccga taatgaaaag cgggccagag aacggcattg gagaatttgt tgtagtattc
     2101 gtcaaggtcc tgttcgctga ggttaaaaga ggcccacgta atgttaccct ttttcacctt
     2161 ttttagcggc tgatcctcat tccctgtttc accgctccag ccaaaccaca gtccacctgc
     2221 ggctttcagt gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc
     2281 tggtggtgca atccggttag atactacgac taaacgactc atagtcatca ctcctgttat
     2341 tttctctttt ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca
     2401 cgcccgccag tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc
     2461 ccagccggtt aacgactgcg aagccagatt catcggttag atcatcgccc agaaatacgg
     2521 gcgttcgccc gataaaggga gcttcctgca taaaggcagt aatcgcctca cctttactgg
     2581 tacctttcgg tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga
     2641 tctgagtaat acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctggcgat
     2701 aatgcagcgc aaaagccatc cctttcgcct ccagctccgc gccgggatac tgggcgatga
     2761 ccgtatgcag ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgcg
     2821 ttttaccatt gatgtcacgg cgctccgccc catgcacgcc cgctaatggg aagcgataag
     2881 gttttgccag tgcgtcaagc tccaccattg agcgccctga aatcaatgcc aatgcaccat
     2941 cacttgcggt tgccagtagc tgaagtcctt gcagaatagt gtcaggcacg acgacctgat
     3001 cgggatgcgg tttgatttcc gccagcgttc catcgagatc aaaaaaccag gcatatttcg
     3061 cggatagttc aggggtttcg gttaacggtt ctgtcacccg gttctcctcc ttctcttttc
     3121 ttattgtgtt gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt
     3181 cgccatttgt aacgaagaac agccaaccgt tgtggcggct ggctgtggag gaaaaaagcg
     3241 ctaaattgtt gcagaaaaaa gcatcagaca gtgcgtttcg ctttttgctt gtaacggtcg
     3301 aagatcaccg ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata
     3361 ttaagcaggt tcatggcgtt ctccacggtg cctaaaatta agatacccgc caccacatat
     3421 gagatttttc cgatgccgcc tttcagagaa acgccaccta aaacgcaggc ggagatcaca
     3481 atcagctcat aaccaatcga cgtcattggc tgcccactgg tcatacgtga agccagaata
     3541 attccggcta tcgctgatac caggccagag agaacaaaga taataatttt ggtgcgaaca
     3601 accggtacac ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtattacga
     3661 ccaaaggtgg ttttattcag cagcaagcca aagatgatca aacacgcgac ggtgagccag
     3721 attggcgcag gcagaccgaa ccagttggcg taaccaaggg caaagaagct ttcatcttcg
     3781 ataccgaccg ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt
     3841 gccagtgtcg tgatcagggc atttattttg agtttggcga taacaaagcc attgaccaga
     3901 ccacagagaa tgcccagcag caaccccgct gccacgccaa tccacaggct ttcagtcaga
     3961 ttaataacca ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca
     4021 aagtcaccgg aagcaaggca gaacaacatg ccacaagcca ccatccccga catggaaatt
     4081 gccaggccca accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag
     4141 gcgataaaaa gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag
     4201 ctgaatgacg acttaggtgc gccagaaccc gatgtagaaa cagaagacat catactctcc
     4261 ttactcaggc aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat
     4321 ctgcctgctc gtgtaacaat tcaccggcga tttcgccttc ccgcatcacc acaatccggt
     4381 cggcaacgcc gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg
     4441 ccgccagcgc ataaatcacg ttgtaaattt catgcttagc accaacatca atgccacgcg
     4501 taggttcatc cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt
     4561 tttgctgatt tccgcctgag agattcatga tcagttgctc agcgcccggc gttttgatgt
     4621 tgagcgaacg aatgtggtga tcggcattgt tttcttccca accgttgttg attacacaac
     4681 cgccaagcac atgtttacgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg
     4741 gaataatgcc ttccgctttg cgatcttccg ggcagagcat catgcctgcg gcaatggcgt
     4801 ggctcggttt acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt
     4861 gcgtcccgcc aaacatgcct ttcattaatt cgctacgccc tgccccgacc agaccaaaca
     4921 gaccgacaat ttctccactg cgaaccgcca gacttattgg cgtacgcacg cctggtgctt
     4981 tcacagcatc aagacgcagc cgctcctcgc cataactacg cggttgccag ccgtagatat
     5041 cgccaatgtc tcgcccgacc atcgcctgca ccagtgcgtc gtggtcaacc tgctgcatat
     5101 tggtaaaagt tttgacataa cgcccatctt taaagacggt gatggcatcg ctgagggcaa
     5161 atatttcttc catacggtga gaaacgtata agatcacccg cccctctttt cgcaattcac
     5221 gaataacgcg gaaaagattg tcgatttcgc gggcagagag ggagctggtt ggctcatcaa
     5281 aggcgataat tttggcgtta cgcgccagcg ctttggcgat ttcaaccatc tgccactgac
     5341 caatggagag atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt
     5401 gtaaacctgc ctcataattc agcaatgaac gattcacaat gccgccttta tgcggcagct
     5461 ggccgagata gatattttcc gcgacggtca tttccggcac gagatgcagt tcctgataaa
     5521 taatcgccac acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat
     5581 taatcactac agaacccgtg gttggcgcat agtttccgct gaggattttt aagagagttg
     5641 attttcctgc gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac
     5701 taatatccgt cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat
     5761 acggggtaga ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca
     5821 tcatgcagag gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt
     5881 cttctttaaa gttgtcacgc gtgatcagca ccacgtcggt aacttcggtg aatttggtcg
     5941 gttcaacacc ttttgcaacc cagttgtaaa gcatttcgct ggacttataa ccatgtacgt
     6001 ccgggcttgg cagcagggaa ccgtagaagc cggttgcctg cgccttagac agttcactca
     6061 ccgcatccac accgttaata ccgataccga tgatatcggc cgctttaaag ccctgacctt
     6121 ccgtcgcgcg tacgccgccc agcacggtgc tgtcgttcat accgacaatc agccaatgtt
     6181 taacttccgg atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt
     6241 tagatttggt cggtacctga taaatttgtt tttccgggaa tcctgcagct ttcagcgcat
     6301 ccatagaccc cgtagtacgg cgacgagccg tatccagttc gttggaggta atcgccatca
     6361 ccgcgctttc tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctggccct
     6421 gacgttcgcc aattttggtc gcagccatca tcaccaacgg aacggtatcc attggcttac
     6481 ctttggcgtt aacaaactgg tcatccacgg caatgacttt catatcgtag ccacgcgctt
     6541 tcgccacgat ggcagagccg agtttcgggt ccggagtaca aataacgaaa cctttcgcgc
     6601 cactggcggc caggctgtcg atagcgttca atgttttttc gccatccggc acggcaatct
     6661 taataacttc aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga
     6721 accacggctc ttccggttgc ttcaccagga aaccgagctt caggttctcc gccatagcgg
     6781 attgtgacat aacggctgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg
     6841 ttctctccag ctttagtgtc gttttgtgta gggcaaaaac gaatgacatt cgttaaatta
     6901 atcggaaaac aaagcattac cttttaacta aaagataagt gactgtgttg acatagtttt
     6961 agcgagaaat taattctcca taggagagca atatcacatc gcagaattac agtgagaacg
     7021 tgcataaatt tagcgggaaa agacataagg aaaagcgaat ttgtcagaca aattatcgag
     7081 tgcacagcag aataatccat aagattagcc tggaagtcct tgttgtcttt ggtacccatg
     7141 cgggatgtct tctttttaac cagtcaatag gcctcattac ctggcgttga gtttttgcaa
     7201 tggtgtaata accgcaactt aaagatgtgg aaaatgcgcg tcattcattt cgccattaat
     7261 tatcactgtg ctcattaatt aacagaacac gtataatgag agccatatcg caaaaatgaa
     7321 aaaacgtttt ataaaatcat tgcctcatca tgaattcaaa ttcatcgatt aatatcaata
     7381 agatacaaaa ggcaccatca ttaaaattca ttacagttac attgatttca tcaatgaaat
     7441 gtaaaaatat aaacttgatg atttaagcat tttcttatac ccgttcagac gttattctta
     7501 tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag aaaggaatta
     7561 ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact tctggatata
     7621 aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca aatgaaccgc
     7681 gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga acagagtctg
     7741 aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca aatgatgcgc
     7801 atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat tgatgttccc
     7861 ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga atacgagcaa
     7921 cgttcaagca cgctggcaca gttagccgat gaagcgaaag aactgaatga tgattcaacc
     7981 gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggcctgtt actgcaaacc
     8041 attcttgatg aagtgcgcag tgcgaaactt gctggtatgt gtcctgtgca gaccgaccaa
     8101 catgttctga atgtcgtgtc gcatcagcta cattgatcat catcggcgct aatgcattgc
     8161 gccgatgaag tttttgagaa accgctgcct catttttttg aagcagcggt tttttaatgg
     8221 gattcgccct gtggggtaaa cttgagttca atcaacgcga tggctttttg gattgcccgc
     8281 atggtgaccg ggtctgcggc ggcgggatgg ttagtaaagt cgatattctt cagctggctg
     8341 gacatttttt cgcgaacttc aacgggcgcg atcacatcga gaacatccag aatttgtttg
     8401 ataaccaact ggcaagcaac cacatcagaa accagttcct gatcggcatt cagcggctgg
     8461 gacatcgtaa actcctgata gcattttgaa agccgttata gtagcggctt cacatcttca
     8521 gcgatagtca catccaccgt catcagaaca caaaaaaacc tgccggagca ggttttttgt
     8581 tatcggaaca tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt attgaacata
     8641 cttttcagga tcttgcgcag tttcatcgcg gtactccgac catttgttat acaggtgtta
     8701 ttgtctttgc gtttataata tgacaaccat cacaaaaatc aatctttatg tgatgtaaat
     8761 cacataaata cccctttaat gttataaaaa tgataatcaa aaaagcagcc ctccaatttc
     8821 tgacacatac agatggcaag aaatagcgcc tgccaggcgt cttttccggc cattgtcgca
     8881 gcgctgtaac gcgtaaaata ctgctttctt ttactcttct ggctggacca tgagacttct
     8941 gattctgact ctttccttaa taacgctgac ggggtgtacg gttactcgcc aggcccatgt
     9001 gagcgaagtt gacgcggcaa caggtattgt tcggttggtt tatgatcagg cttttttgca
     9061 gcatgcccgt accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat gccagcaggc
     9121 aggctatacc cacgctatcc cctttggtca gccagtaggc aactgtagcc tctttgcggg
     9181 ttccttatgt ttgaatacgg aattcacttt gtcatatcag tgccatcatt ccgccttccc
     9241 tgtctttctc taaacgtttt aatcagcgag gggg
//
LOCUS       DAOFSV010000164         9169 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.164, whole genome shotgun sequence.
ACCESSION   DAOFSV010000164 DAOFSV010000000
VERSION     DAOFSV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.164"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(513..695)
                     /locus_tag="QBH27_004076"
     CDS             complement(513..695)
                     /locus_tag="QBH27_004076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016327.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            complement(902..1228)
                     /locus_tag="QBH27_004077"
     CDS             complement(902..1228)
                     /locus_tag="QBH27_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HDQ7016328.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            1709..2002
                     /gene="iss"
                     /locus_tag="QBH27_004078"
     CDS             1709..2002
                     /gene="iss"
                     /locus_tag="QBH27_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDQ7016329.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2093..2275)
                     /gene="rzoD"
                     /locus_tag="QBH27_004079"
     CDS             complement(2093..2275)
                     /gene="rzoD"
                     /locus_tag="QBH27_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDQ7016330.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2492..2968)
                     /locus_tag="QBH27_004080"
     CDS             complement(2492..2968)
                     /locus_tag="QBH27_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDQ7016331.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTLRESEV"
     gene            complement(2955..3260)
                     /locus_tag="QBH27_004081"
     CDS             complement(2955..3260)
                     /locus_tag="QBH27_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDQ7016332.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(3582..4271)
                     /locus_tag="QBH27_004082"
     CDS             complement(3582..4271)
                     /locus_tag="QBH27_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ7016333.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQLYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     IFSQQDIAKLD"
     gene            complement(4268..4408)
                     /locus_tag="QBH27_004083"
     CDS             complement(4268..4408)
                     /locus_tag="QBH27_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDQ7016334.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPSSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(4405..4767)
                     /locus_tag="QBH27_004084"
     CDS             complement(4405..4767)
                     /locus_tag="QBH27_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ7016335.1"
                     /translation="MNTYCITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(4764..5054)
                     /locus_tag="QBH27_004085"
     CDS             complement(4764..5054)
                     /locus_tag="QBH27_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDQ7016336.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(5047..5217)
                     /locus_tag="QBH27_004086"
     CDS             complement(5047..5217)
                     /locus_tag="QBH27_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016337.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(5217..5672)
                     /gene="ybcN"
                     /locus_tag="QBH27_004087"
     CDS             complement(5217..5672)
                     /gene="ybcN"
                     /locus_tag="QBH27_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HDQ7016338.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(5669..5770)
                     /locus_tag="QBH27_004088"
     CDS             complement(5669..5770)
                     /locus_tag="QBH27_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016339.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHLASDLCGGEA"
     gene            6120..7163
                     /locus_tag="QBH27_004089"
     CDS             6120..7163
                     /locus_tag="QBH27_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7016340.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            complement(7200..>9169)
                     /locus_tag="QBH27_004090"
     CDS             complement(7200..>9169)
                     /locus_tag="QBH27_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDQ7016341.1"
                     /translation="AGSQAGTVRVTAKVQGKTFTLSIKQTATTEPDSEVSAVLTAAPA
                     EQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYSATVTSK
                     RAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGKQRNMVTVSL
                     ADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGSYVITAHAGS
                     QMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNTNASVNGQKI
                     QLITSSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVESGSVKLTFV
                     PDVASAELNMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITPSTGASLTSS
                     NLQLDAHGQATTQFTASKSGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVLEITSSA
                     SSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTTVTTDENGQA
                     TVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADDANGVLTLTKDPGYETNDGSPV
                     GIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEFSTYESG
                     YHWIKAKITNSKNTYEKTIRTYVVKLPEKDS"
BASE COUNT         2423 a         2278 c         1993 g         2475 t
ORIGIN      
        1 cccgttttca gcacctgtcg tttcctttct tttcagggag tgttttaaat aaaaacatta
       61 agttacggcg aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa
      121 cccgcgaggt cgccgccccg taacctgttg gatcgacgga aaggacccga aaacgagaat
      181 aattatcact tacagcaaga atcgaatctg atctattatg gtgcttgcta ttatgtgccg
      241 gcacaagtgc gtcgtttacc gtcatttcac acagaggcat catcaaatga aaatcagaaa
      301 tattctcgct atctcccttg cgacatcatc cttcagttgc ctggcattta aatcctcgcc
      361 caatgtgcta ccaggaccaa cgaatcaact aactgcggta gaaagtaaaa ttatcggaca
      421 tttttatgcc ccacacagta cattacccgg aacaaccatc acagggacat gtgacgcctc
      481 ccccgtcccg ggatgcacct gtccgttttg tactatgctg cgtagccaaa accgataaca
      541 tccgcattta cctggtattc catgatgagt tcacgcagcg actgatagag gaaggaaaga
      601 tggttagtaa aagcaaggcg cattgccgcc gcatgctgca ggcattgcaa cagacgagag
      661 caggtatttt tgaccagttg gaaaactgcc agcatacttt gcccgagtat atcgccatct
      721 catcggaaac cagtgcaact cttattcatc gggttccacc agagaaaaag aagaaatgaa
      781 cagtgaggcg ttgtgtggca tacaacgcct tcttccatca ttcctcgtca gccatgacaa
      841 aaatataacc gctggctctt tcatttttct cctgcttcca gcccctctct acctggaagc
      901 atcaaggacg tgacggcgta aagataaatt gtctcttcac tccctgacag gggcgattct
      961 tttcaaatcg ccatttcgcc atggccttca ccacttcatc acgaaacaaa ttatgaggct
     1021 ctgagcggag aaaaacgatc cgtgtcacag tcccatcagc accaatatcg aacttaacct
     1081 caaccagccc cttgatataa tttgctgcag catattccgg atatcgtgga tacaccgtca
     1141 ctaattgccg gggctcatca gctttttgct gcgagcatcc cactgccagg acagataaca
     1201 gaaaaagtag taaaaggcgt cttttcattt ttattcctac gggtcttatt ctgacaatat
     1261 atcctgtgtt ccagactgcc acatcaccac atcctgtgcc attatctgac tcacattaca
     1321 tacatcgcat cgggatacag tagtagcact ttctgtaata cagcttcctg tttcttccac
     1381 catcgcaccg ggataaaccc gcgaatcatt aacgcggtaa aaacccggtg tgcatcgttt
     1441 ttaattattc ccgcacactc acgcagaagg aattccccgt cgggctacgg tcatggttaa
     1501 tgcgggaata cggcgacgat acagcgcagc taaaagggta atggacggat agaccggttt
     1561 atttcattcc acaggattct gagtgtcccc aacttcctcc aatagtctga gcccacctgt
     1621 gtagttttaa ttttcatcaa tccatttaac tatcgtttaa ttgttgtcac ataggattct
     1681 gccgttttta acaatgcagg ataataagat gaaaaaaatg ttgttttctg ccgctctggc
     1741 aatgcttatt acaggatgtg ctcaacagac gtttactgtt ggaaacaaac ctacagcagt
     1801 aacaccaaag gaaaccatca cccatcattt cttcgtttcg ggaattgggc agaagaaaac
     1861 tgtcgatgca gcaaaaattt gtggtggtgc agaaaatgtt gttaaaacag aaacccagca
     1921 aacattcgta aatggattgc tcggttttat tactttaggc atttatactc cgctggaagc
     1981 gcgtgtgtat tgctcacaat aattgcatga gttgcccatc gatatgggca gctctatctg
     2041 cactgctcat taatatactt ctgggttcct tccagttgtt tttgcatagt gatcagcctc
     2101 tctctgaggg tgaaataatc ccgttcagcg gtgtctgcca gtcgggggga ggctgcatta
     2161 tccacgccgg aggcggtggt ggcttcacgc actgactgac agactgcttt gatgtgcaac
     2221 cgacgacgac cagcggcaac atcatcacgc agagcatcat tttcagcttt cgcatcagct
     2281 aactccttcg tgtattttgc atcgagcgca gcaacatcac gctgacgcat ctgtatgtca
     2341 gtaattgccg cgttcgccag cgtcagttct ctggcatttt tgtcgcgctg ggctttgtag
     2401 gtaatcgcgt tatcgcggta atgattaacc gcccatgaaa ggcagacgat gacgcagata
     2461 accagagcgg agataatcgc ggttattctg ctcatacctc actctctctg agcgttccgc
     2521 cagcttcttt gaattttgca atcaggctgt cagccttatg ctcgaactga ccataaccag
     2581 cgcccggcag tgaagcccag atattgctgc aacggtcgat tgcctgacgg atatcaccgc
     2641 gatcaatcat cggtaaagca ccacgctctt taatctgctg taatgccact gcgtcctggc
     2701 ttttggggga gaagtctttc aaaccaagct gtttacggta agcatcccac cagcgtgaaa
     2761 gaagctgata acgtccggcg gctgttgatt tgagtttggg gtgtagcgtg acaagtttgc
     2821 gagggtgatc ggagtaatca gtaaacagtt cgccgccaac aataacatca taaccgtggt
     2881 tacgtgtcgg ttgtcgcccg ttatccgttc cttctgacca cgccaacata tcgaggaagg
     2941 ctttacgctg aggattaaga ttttgcattt ttcacccctg tcagtcgttc ccagaagtac
     3001 gtcagtgcaa ccgaacccat cgcaccacta atccccgctg tcgcgagaat catgtaaata
     3061 ctgaatccac tttcgatgct gatcaggcca ccaataacac cggtgaatcc tgataccact
     3121 atttgagcca gagcatttat ccaactccac gttgctttac tctgcttcac atctatcagg
     3181 tagcggacca gaccgcccca acctgcgatg atcagcaaaa cgagccagaa cgctccggca
     3241 aggctctctt tttcgtgcat atgaatagcc aatgtttcgc cgccgacaaa aggccgggac
     3301 gttaaatgtc agaaatcagg ctcacggggt aatttaacga caaagcacgg agttgatgct
     3361 ccccgcaagc ctggaataaa aaagccagca tgtagctggc aacagagggt taagcaatat
     3421 caactcaaca gctgaagaca ccctggctgg ggtaggttgg aaggctactc accgttcaga
     3481 aacagaaaag cccaaggctt taaacctcgg gcttgaattt ggattactgc cagtgcgtac
     3541 aacattggca aaatatcaga tttacataaa atatatgatt tttaatccag ttttgcaata
     3601 tcttgctgtg aaaatatggt cttttgtttt gaacgtgttt tcgttaaaag caataaagct
     3661 tggctatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt aaatgtctca
     3721 gaccagtttt ttgatgtcac tcccaccagt gatgccagct cctggtattc ataggtctta
     3781 cgccctgcca gctcgttctt cacatcctgt gccgccagcc agatcaactt cttcaaacgt
     3841 tccagtgttt tacctgcaat tttcctggta cccaacagag tcttaaactc gctccatgcc
     3901 cactgcgtta tggtgacctg atgttcccag cgaacacttt cgctgtaact ccacagcaac
     3961 cacgctttct gatgttcttc gagagacaga accgcgcggc gccatgacga ggttgagaac
     4021 tcaacctggc tgaccagtgc aatggatgaa ccttttgcgt acaactgctt accggaaatc
     4081 ggcggattat ccagcgtaat catcctgcca gttacctcat ccagaatgcg cggcttcttt
     4141 cgtttgtatg taccagtatc aaattgtgca tgctccagcc aggcttcaag ctgtcctttc
     4201 gttgctccgc tcaaatcagc ggtagccaca atgagttgct cgcggacata ctgtaaatat
     4261 tgggtattca tgcggcagct cctttcagtg ttttggcgta attcttcagt atccggtaat
     4321 cggtcaaaac agaactagga aaacgatata agcgcaggcg cacccagcgg cggcgaagac
     4381 gttctgccat ataagactca aacatcattc atctcccagt tcagtgatag tcagctccag
     4441 cttcccacct ttggtaacgg gcatcttcac aacgcggtaa tcaacgacct gagcatcatc
     4501 cagccagaaa cctgctttgg tgagtgcgtc aaaagcggcc ttttgcagat tatcaaggtc
     4561 acggcgacgg cgatccggca tgtggcactc aatgctgatt ttcacaggaa tagccaggcc
     4621 gatatccagc attgcgtttt taatgattcg ggcgacatta tcgcggtatg cctgcccctc
     4681 tgcgctgata tgcgtgcgtc cgcgattatg gcggtaatag cgattattgc tcggcggcca
     4741 gggtagtgtg atgcagtaag tattcacgcc ttaattaccc cctctttcag ccagataacc
     4801 tgtgttctcg ccataccttc cagcgcacat tcttttgcat atccagcgtc aacaaaatgc
     4861 gtgcgacggt cgatttcgtc gtggcaggca gaacatgcaa tggtggcaat caggtctggc
     4921 ggtttgatac cggtaccgca caatccagcc agccggatat gtgccagtac tgacgtttca
     4981 ggattgccat tacatacgcc agggattctt acctggcatt cccgaccacg cgctgctttt
     5041 ctcaagtcag ccatgattcc tccttgctgc cagtcgcaac cattttttat caaccaggct
     5101 ggcggtatat ccgagcagtg ttggtatttc ggatggtttc agctcaggct tacgcttacg
     5161 acgatttggt actctgtaga tgtgtccgtt catgacacga ataagcggtg tagccattac
     5221 gcctcctgct tgtcgcgcag cagctggaac tcgcagctct gcggaatagt caggtggcag
     5281 ccaatattca ccgcccaggc ttcaacctta cacaggaaga catacatctc tccggtatcg
     5341 agatcggagg tatggcgtaa cgactggata gtagtgattt cgccggttac gacatcaacc
     5401 aggtccttgg tttcataacc gaggtatgtg tgtttgagag catcttttac ccatgctgcg
     5461 gtagcgaacg atttccccct gctgatgagg tattcactga tttcgctgta ccacatgtgg
     5521 ctgagtgcat tctgggaaag actgcgtttc tcacgccacg gtttaagcac catgcgaaag
     5581 cattttccgt tctccagata aggctggatc tgctggccga tagcgatgaa gttaccgcga
     5641 tgcagtttga tgccatcttg tggtaggttc acgcttcacc tccacagagg tcagacgcta
     5701 gatgcaaaaa aacgcaggtg catttctgca tctgtgaagg gagaagagag tttggattgt
     5761 gtgtgcgcat aaacgtcccc gtttagcgca gaagtcaccg gagttgttca ggctccggtg
     5821 atacaattat ggcgaattga ttattcataa tcaaacaaga taaggtctca aacttcatgc
     5881 aagccaagat ttatttctga cagaattata caaagaagct attggtcaga atctactcgg
     5941 actgtaaaac atacgcataa ccttaagctc tcactttaag cattgttgaa ataatagccg
     6001 tcaagtacaa ccttaaccac gactgggata tttccctggc taccacgagt tgtacggcta
     6061 ttaaactgcc gttaaattta gtaagagaat ttcatccgat aagtcaaggc atgtaaaaca
     6121 tgaaaattaa caagatatta tcatctgcaa cactattgta tggtatgtca atggccatgt
     6181 cggtcgggag ttgtgcaaca cctgtccaga ctaatcttcc tggttacacc ccgggtgcag
     6241 atatcattag tgtttcaccg accagaaacc aggtcgatct cattggtgat gttgtttatt
     6301 cccagataaa aggaactcgt tctgtcagac agcttcacat gtcagttctt gtcccgcgaa
     6361 caaatgattt aaaaccagcc attatttatt atcccggcgg cggattcatg tcttctgaac
     6421 atgacaaatt tattgaaatg agaatggctc tggcagaagc tggttttgtt gtggccgctg
     6481 tagaatacag aacaattcct gatacatttc cagcaccagt tgaggatgga aaagctgcaa
     6541 tacgttacct gagagagcat gccagcaatt atgggattga tcctcaaaga atcggagttc
     6601 tgggtgactc tgccggtgga tggcttgccc agatgatggg aactacaaat ggtgacaaaa
     6661 cctttgataa aggtgacttt cttcagcaat cctcagatgt tcaggcagtt gccacacttt
     6721 atgggatttc tgacttgttg aatattggcg aggggttccc tgaatcagtg caggaggttc
     6781 atcgctctcc tgccgtaacc gaagccttaa tgatcaatgg ccctgcattc agaaattttg
     6841 cgggagcccc catcacagcg tcaaaagaaa aagcgctaaa cgccagtcca atcggacata
     6901 tgaaaggagt aaaaccccca tttcttatta tgcatggtag caaagacact ctggtttcac
     6961 ctgagcaaag cgccaaacta ttcaggatgt tgaagaagaa cggtgataac gctgagtacg
     7021 tgctggtaga aggggccgag catggcgata agacatggta tcagccaatt attataaaca
     7081 gagtcgttga gtggtttact aaaaacctgg gagcgcctat aaaaacagct ccccaacaac
     7141 aaaacccaaa cgctaacctg taaaaagagg gagggctaag ccctccccat tcaattttgt
     7201 taactatcct tttcaggtag ttttacaaca taagtcctta ttgttttctc atatgtattt
     7261 ttgctattcg ttattttggc cttaatccag tgataaccac tttcataggt gctgaattcc
     7321 gaagctgcat tacctgtcca gtgtaacgtt actgtagcag gctccatttt gacccgttga
     7381 gagtctggac tatcttcact accagcagaa aactcaaccg taccagataa tgggtttcca
     7441 aaatgatcga caaatcgagc ataaatacct actggagatc catcgtttgt ctcataacca
     7501 ggatcttttg ttagagtcaa tactccattt gcgtcatcag cgtacaggga gaatgatttc
     7561 accgcgctaa catcactgcc aagttcgttt aaggtcgccg ttaatttata cgaaccaacc
     7621 gttcgaccat gtacgcttac cgtagcctga ccgttctcat cagtcgttac tgtggtttta
     7681 tcaaccacca acgcaccata tttagacggc ccagatgtct taacattcaa tgctcgacca
     7741 cttaatgctt cgcctgactt acttttcaac agtaacgtaa ataccagatt gttggtatcg
     7801 ctcactacag ccgaagatgc agatgatgtg atctccaaca cagccccctt cacatccgtc
     7861 acggcatcaa tattctggct tgcggttatg cgtttcccat ccagaacata ctcggcctga
     7921 attgtgtact ggcctgattt cgatgcagtg aactgcgttg ttgcctgtcc atgagcatcc
     7981 agttgcagat tactactggt caatgatgct cccgtcgatg gtgtaatcgt taaatccacc
     8041 tcgcctgtaa acgcattgtt attcgcatca accaactgaa tgtttactgt tgcgttttca
     8101 ctgccatcgg ccacaatctc ctgttttgat acactcatat tcagttctgc agaagcaaca
     8161 tcgggcacaa aagttaactt aacgctgcca gattccacgc tatgggaacc gtcagtcacc
     8221 cgtgcagtca ccgtgtactc accagctttc accgttgtaa gcggaacaga aacatgccct
     8281 gttgaatcag tcacgatatt tgttggtaca gataatcctt cagatgaggt gatgagctga
     8341 atcttttgcc cattgaccga cgcattcgta tttgtcagct gcacatctaa taccgctgca
     8401 tccttaccat tagcaggaat attggaaatt aaactactgc tttcaggcgt cagtgacagt
     8461 gaagctccgg tcatatttga tgcaaaggtc actgttaact cagtagacat ctgagaacca
     8521 gcatgagctg taatcacgta agaccccgga gtggagctta ttaacgcaaa aatagcatta
     8581 ccattttcgt cagttgaaac agcatgttca ccaccaactt gagttatccc tgctggtaaa
     8641 gacaatgtga cagcataacc aggaacaaca ttgccgaaac ggtccgcaag gcttacagtt
     8701 accatatttc tctgttttcc atcagccaat gcattatttt ggctggcttc aaactgagag
     8761 aatgtaacct gctgccggtc ctcaataaag gtgatttctt tcttaacacc tgagaaatca
     8821 tgactatcag atttaacacc gatagtaatc ttaccagctc gttttgaagt tactgtcgcg
     8881 ctatagacac cgtctttttc cgttacagtc ccaaactcga ctccttctgc ctggttaaga
     8941 gcataaaagc tcagagtgtt atcaccagtg attgcatttc cttgtgaatc cttcactgcc
     9001 agttgtagat tgatattgta accaactacc tgttctgctg gtgcagcggt taaaacagca
     9061 ctgacctcag aatcaggttc tgttgttgct gtttgcttaa tactgagagt aaatgttttt
     9121 ccctgaactt ttgcagttac acgtaccgta ccagcctgcg aaccagcag
//
LOCUS       DAOFSV010000165         9136 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.165, whole genome shotgun sequence.
ACCESSION   DAOFSV010000165 DAOFSV010000000
VERSION     DAOFSV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.165"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(137..268)
                     /locus_tag="QBH27_004091"
     CDS             complement(137..268)
                     /locus_tag="QBH27_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDQ7016342.1"
                     /translation="MIAYCFCEWHCCSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(768..844)
                     /locus_tag="QBH27_004092"
     tRNA            complement(768..844)
                     /locus_tag="QBH27_004092"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(808..810),aa:Arg,seq:tct)
     gene            complement(945..1020)
                     /locus_tag="QBH27_004093"
     tRNA            complement(945..1020)
                     /locus_tag="QBH27_004093"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Ile,seq:cat)
     gene            complement(1070..2119)
                     /locus_tag="QBH27_004094"
     CDS             complement(1070..2119)
                     /locus_tag="QBH27_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDQ7016343.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRAIGVELET
                     GRFEQTVREVQGLIV"
     gene            complement(2271..2468)
                     /locus_tag="QBH27_004095"
     CDS             complement(2271..2468)
                     /locus_tag="QBH27_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDQ7016344.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATAGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(2695..3516)
                     /locus_tag="QBH27_004096"
     CDS             complement(2695..3516)
                     /locus_tag="QBH27_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ7016345.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCRGEKFIEGEVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKIREVVKVSCPECKGKGEISTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(3513..3893)
                     /locus_tag="QBH27_004097"
     CDS             complement(3513..3893)
                     /locus_tag="QBH27_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ7016346.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMHEEQVKK"
     gene            complement(3894..4949)
                     /locus_tag="QBH27_004098"
     CDS             complement(3894..4949)
                     /locus_tag="QBH27_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ7016347.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(4951..5223)
                     /locus_tag="QBH27_004099"
     CDS             complement(4951..5223)
                     /locus_tag="QBH27_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016348.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(5391..5546)
                     /gene="hokD"
                     /locus_tag="QBH27_004100"
     CDS             complement(5391..5546)
                     /gene="hokD"
                     /locus_tag="QBH27_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDQ7016349.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRLRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5784..6449)
                     /locus_tag="QBH27_004101"
     CDS             complement(5784..6449)
                     /locus_tag="QBH27_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDQ7016350.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(6624..7049)
                     /locus_tag="QBH27_004102"
     CDS             complement(6624..7049)
                     /locus_tag="QBH27_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDQ7016351.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(7065..7835)
                     /locus_tag="QBH27_004103"
     CDS             complement(7065..7835)
                     /locus_tag="QBH27_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDQ7016352.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(7857..8603)
                     /locus_tag="QBH27_004104"
     CDS             complement(7857..8603)
                     /locus_tag="QBH27_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ7016353.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            complement(8610..>9136)
                     /locus_tag="QBH27_004105"
     CDS             complement(8610..>9136)
                     /locus_tag="QBH27_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7016354.1"
                     /translation="IRGPMIDPDPSVLKPDPSDKRSSCPDASQPDPQTAEQDFLTRHP
                     DAVVFSAKKRQWGSQEDLVCAQWIWGRIVSLYEQAASYDGEITRPKEPNWTAWANDVR
                     TMRMLDGRTHRQICEMFGRLQRDSFWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNH
                     ISEPDTEIPPGFRG"
BASE COUNT         2159 a         2595 c         2037 g         2345 t
ORIGIN      
        1 ctactcgctg attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac
       61 tccccgcaaa ccagacaaca atcaaccgcc tgaattgtga agtatttaaa aatttctccc
      121 gctaactgat acccggctaa cagtctggcg ttttcttttt cagcaacggg aaagcaacaa
      181 ccaccacacc cgccaccagc acaccgtcag ccagcactga cattatccgg ctgcagcaat
      241 gccattcaca aaaacagtaa gcaatcactt tttaccgtaa ccggtgataa tccagatatg
      301 tatctacccc agatgagtaa tccgaagttc atccatacca caggtcctgg ctattctgtt
      361 gtactcctga acaagagtaa ataattctga attagccacc atgaactcat cgcaaaccct
      421 ctgtatagca tcactattca gaataataac gtctcttccc gaaagacgat caggagtaca
      481 gaacaaaact gtcaaacggc tgaaggcctt tgctcgtgct gcattgacta tatcaatacg
      541 ctgcctaagg atgaaacacc ccgacgcctc atcaatattc actctaccca caccatatga
      601 atgataaata tttaatgctg aaaaaaccat tagaccgtat aacaaacact caatcaacac
      661 ttaacagaac ttttattttt gacaaacata taatattttc aacaatatcc tgagccaggt
      721 atatttcagt ataaggctct gccggaagga atctggaaga atgaatatgg cgcgctgtac
      781 tggattcgaa ccagtgaccg attgcttaga aggcaattgc tctgtccggc tgagctaaca
      841 acgctgaata ccgataatgg accgccatcg gggacccgcc cccgcaccaa caaccctgtt
      901 atcgtgtcgt ctgctcttcc tgataagcta atggcggttt gtgatggtgg cccttgctgg
      961 atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1021 gggagcagaa taataatggt gcgtaattaa ttctgcaatc tcatccgttt caaacgatta
     1081 aaccctgaac ttccctgact gtctgctcaa aacgtccggt ctccagctca acgccaatcg
     1141 cacgacgccc gagcgccagt gccgctttta ccgttgaacc tgagcccata aaaaaatctg
     1201 caaccaggtc acccggacga ctgcttgcgc tgattatctg ctgcagcatt tctgccggtt
     1261 tttcgcacgg atgtttcccg ggatagaact gcaccggttt atgtgtccac acatccgtgt
     1321 acggcacctg cgccgtcaca ccaaaatacc gccgcagatg cttatattca ctctgcagct
     1381 ccacatactg ccggttcagt gacgtatacg tatccaccag ctggtggtgg ggcttttcca
     1441 gttcaccgcg ctgatgtttc tcttctgcca cccgggcaaa cagcgactgt aatttcagat
     1501 aatcgctttc gttcggtagc tgccactgac tggcactgaa ccagtgcgac accatgtttt
     1561 tctttcctgt ggcatctgca atctgttttg ccgttatccc cagggccgcg cgcgcatcac
     1621 gaaagtaaga aatcagcggg gccatcacat gctgtttcag tgcactgccc ttcgccgcat
     1681 acccggcatc tttcggacga tacggcccct gataatgttc cgcgaacaga atgcgctctg
     1741 tggcggggaa atacgcccgc aggctttcct tgttgcatcc gttccagcgt ccggacggct
     1801 tcgcccagat aatatggttc agcacactga agcgttcacg catcatgatt tcgatatcag
     1861 atgccaggcg atgaccacag aacaggtaaa gacttccggc aggtttcagc acccgccaga
     1921 actgcgccag acactggtcc agccacttca ggtaatcatc gtcgcccttc cactggttat
     1981 cccagccctc aggcttcact ttaaagtacg gcgggtccgt gactatcagg tcaacagaat
     2041 tttcgggtaa cgaccggata aattccaggc agtcggcgtt gattaactca caactggata
     2101 tttttacagt attagccata gatcaataag cacttctctg ataggctcat accgcttttg
     2161 cgcaaagcag atgggcctga ggtttgcttg tgaccccaac gcatgagcag atggctggtg
     2221 ggtgccccta acacccacca gccgcccatt taccacaaat aaaaaagcct tcactgcgga
     2281 aggcgtctgt aacaaccgaa ctgatagtct gccagacccg ccataaccag ctgggtcagt
     2341 attaactggc agcgttcgcg tgaaaggtaa gtattctgcg caatctcccc ggctgtcgcc
     2401 ggttcggtga cgcttaattc attaaacacc actctggcgg tttctgtcat atcctgctgt
     2461 ttcagcatgt ctttttccct tttccggtta acgtgacaca ccaataactc ttgtcgaaaa
     2521 agccagcaag ctgaaagaca ggtattcacc gccaccagcg cgtttactgt actgacgcga
     2581 tttcagtcat aaaaaacccg ccaggcggcg gggtgtaaaa aatcttctaa cgtcaggcat
     2641 aaaacgccca tcgttagagc aaatttacca cagattcggg aaaaatcaac aacactatcg
     2701 cgttaccctc tttaactgcc gctccgccca tgcctcttca atgtcaaacc gaaccaccaa
     2761 cgtatcgtaa aagcgtttca ctgatttttt ccacgtatca agcgtgatag cactcgtcac
     2821 tttgcatatg gcattaaatg cctccgttga tggtagtctt tcacagccac gaccaccaca
     2881 acgctggcag tctctgataa caggcatacc acgttttacc gactcttcac gatgaatggc
     2941 gacaccacgc ccacggcagt ctttacaggc agtggaaatc tcccccttcc ctttacattc
     3001 aggacaagaa actttcacca cctcccgaat ttttttccat tcttcccagt aagacggata
     3061 cacacctttc gtacactttg cccatactgg cggcttgcca tccggatact gaaccttgtt
     3121 tgtaaaaact tcgccttcaa taaatttttc ccctcggcaa caggggcact gctttttact
     3181 cgctgcgctg cgggcataat cctcaaaagc gtacgaagcc ataatgcgca tcactaccgg
     3241 ttttatttct gccggaagtt ttctcaacgc cgccacacga tcgcaccgac tgagtgcata
     3301 atctgccagt aattctgttg cccgcgccct gtcattcata ctgatgccca ttttccccag
     3361 gaacgcagaa aaccccatct cagcccgatt ctgtgtcatg ccctgcgcgg ccatcacatc
     3421 agtgatactc agcgcatctt tcgacgttga ggccgatgca tcagtcaggc cgggggattt
     3481 tggggagtag tatttcggta aatcttccag tttcattttt tgacctgctc ttcatgcatt
     3541 atggggtaaa tcttcacccc cagacgtcca ccagatactg gctgaccacg aacgatattg
     3601 atttcatcaa actgctcatc gtccattagc aaccccgcat gcgtcagcgc atccagcggc
     3661 gctttcagaa tattgtccag atcacggcgg cgcttatccg gtggttctgc aataattttt
     3721 attgccaacc ttccggacag gcttaatttc agccgctgct ggcgaacaat aagcgccact
     3781 gcccggcgat aacgctcccc ggcttttgat acaaaatatg tgctgccacg acgacgccag
     3841 taagtgttca ccgttggcgg gtaaggcaaa acaaattcta tgcgttcagt catttatgct
     3901 ttccacttca gaacacccga atttctcgcg tgcattaaaa aacgaatcag caacaacagc
     3961 tggctgccgt gtttttcttc aaaatctttt accccggcgt gtagttcgct atggcattta
     4021 cggcacagcg gaataacaaa caaatcatca gcctttgttc ccatccctcc cagtccatga
     4081 ccaatgatgt gatgcggatc atctgcctga ttgccacacg tcatgcattt ctgcgttttt
     4141 acccaacgcg tgtatacagg catctcttcc cgttgtgatt tctggcgctg gagatactga
     4201 gccggtgact ccggatcaac ggcaatgctg accaccgtct tttcctgtgg cgggttttgc
     4261 tggtgggcgt gaggcagcgg cgcaagattt tttgtgcgct gcttcagtat gctggtggcg
     4321 gtctgctctc ccggtacgat gtcgctttca cggtacattg agcggatttt ttccgcacgc
     4381 aaccccagcg aacgacgtaa taccgcttcc ggtagcgcgt ccgccacctg attgcggacc
     4441 gcccaccagg ataattcagc cagcgataat tcccgttcct gcgagccatt cattgcatgg
     4501 cgtatgacgt caatcatcca tgcaaacagg ttttggtgag caagttgccc gagtgattcg
     4561 gaggtctggt cgcgcagctg gttgtcgcag tgccagcaca acaccattgc gccggtacca
     4621 taacggtgaa tgacggtttc actgtggtga taatcgccgt gtggccactg gcaggattta
     4681 acatggcgca gtaaccagtc agacaatgcg ccagcgccac cagcagcacg aatcactcgt
     4741 tcgtcgctga aaaatggcag taatgattta tcctccgcca gcggctggcg aacggcagga
     4801 acgaccccgg acggcagatt acgcatgctt ttcggttccg gctccaccag tacccgggta
     4861 ttgtggaata ccggcatgga ttcacggccc ggcttaacga tcaccagccc gagttccggt
     4921 accagaacag gtcgaagtaa tacccgcacg ttacctccag atgcgttgct ggaatgtgcg
     4981 ggacggacgc ggtgggcgtt cagagtaagg aagcctgacg gagattatcc agtgacgata
     5041 atcgaggctg agggctttct taatctcgta tccgcgtctg cggtagttat gaattagcca
     5101 ttcggcctgt tcttcagtac atggtgggtg ttggtaccag tcggttttaa atgcgtgtga
     5161 acgccgccca tgccggatgg caaggtcggt atcagaattg tgaaatttgg ttttgtgcgc
     5221 catctgtttt ctctgctggc gcagcaggtg tcaggtgttc aggctgacgt gcgaattgta
     5281 aaccagaatg ccagaaaaaa acaaaacccg ccgaaccggg ttaagtgcgg gtgcgttgag
     5341 gatgcctgac acatcagagg tggcgaggga tttctccccc gccgggtctc ttactcctca
     5401 ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg ttcggagtcg tacctcgcag
     5461 aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg
     5521 attaacatcg ccttttgctg cttcatagcc tgcttcccct tgcctttcgg cacgtaagag
     5581 gctaacctac atttgtgaga catagattgg gcctcagatt aatgttaagc gtcttgcagg
     5641 acgcgaaatg ttaactgggg cttttctcta tctgcctttc agtgttcatg cctgagacag
     5701 atagcctcaa gcacccgcag ccattctact taactcacgt cacctcgcca atatgaaatc
     5761 aatcagaaag gtgatccata aaatcactcc ttctcttctt ttccgtagtg gagttggcca
     5821 attttgataa gagggcgtcc ctgagatttg cggtgtagat tggtatcgcg cagagaatac
     5881 acacagccac aatattcctg ctgatagaat ttttcgcgct tgctgatttc aatcatacgg
     5941 gacgagccgc cctgcttgcg ccagttataa tcccagtaca ccatacccgg ataatgcgca
     6001 acagctcgcc gcccacactc gttaacctgc tgcatatttt tccagcgtga aatgcccagt
     6061 gaactgctga tcacactgaa accattttca gcagcgtaca acgctgtccg ctcaaaacgc
     6121 atgtcaaaac acatggtaca acggatcccc ctctcaggct cccattccat tcctttggca
     6181 cgttcaaacc agttgtcggt gtcgtaatca gcatcgataa acggcacgcc gtgttgttca
     6241 gcaaagcgaa tattttcatc cttacgaatt aaatactctt tctgaggatg aatgttcggg
     6301 ttgtagaaaa agatggtgta gtcgattccc gaggcctgaa gcgcctccat cacttcaccg
     6361 gaacatggag cacagcaaga gtgcagtagt agtttgtttg ccccgtttgg gagctccaat
     6421 ttaggccgtt tgaaatcagc aatagtcata aatattttta ttggggtcat gaaaatagca
     6481 cagagtgtag catcagagca gggctatcgg gaatatatgt ctaaatctgg taatatctgg
     6541 ttttgacgca aagcggacaa ccacgctggc tctaccctgc gccatgaaaa tgtcaattca
     6601 catctgaact aatgctcttt aatctagtaa cgtctaaaat acctaacatt tccttgataa
     6661 aatgccagta cacgctgcat agcttcgctc ttccggcact cgcgacagat tatattcagg
     6721 cgcctgtcgt agcggcgtat ttcgccgtct ggtaacgacc agataaggtc cggatcaacc
     6781 actgcaggtt tcttcacctt tgcccttgag agttttttgc gagcattttg ccagtcctta
     6841 cgcgcctgtt cagacgggaa taacccgtaa ccagagttgt atacatcgcc actggcaacc
     6901 agctctctgg cgagaacact catcagatat cttgtcgcac ctgtcttggc ttccagttgc
     6961 cgcaacgtct cgcgaccgct cagacgtaca agttcaacaa cctgcccttt aattttttcc
     7021 cgctcttctt gtgtaaatac ttttgccata agcgcctccg gcaatcactt ttccgataca
     7081 acacggcggg aagaatcagt aatctgtcga acaatatccc ggtgcttgtt cagctcccgc
     7141 agcgcggcgc agactcgctc ccacttctga acatcacttt tcgccctgcg cagcgccagg
     7201 tttgccctgc gaagggacgg aaaaatcagc tcatctgctt gcgtttcggt aaacgatggc
     7261 aacggctgca caatgtccgc cacagtttct gttttaattt cttcctgtgt tgcggcttcc
     7321 cggactggta acgcagcacc tgctggctga ggaaaggcct taccatcact ttccgttacc
     7381 agcgcggctt tcggctctgc tggtaaatta tcgcccggca tgcagtaacg aaatttaccg
     7441 ttctgattaa cgcgtgccag ccgccccgtt gcggttacca ccgccagcgt ggaagcaacc
     7501 ttgcgagtac tgacaccgaa cttacccgcc agttcctcac acgttttagc cccatcctga
     7561 ccgataaact caatcatcat gtctgcggta actttttgtt cgacctcccc ggtcagcata
     7621 tcctgtgctt cagattttac tggccgctct tcggttaccc gggattcacc ttcgccagcc
     7681 agaaaccagg tgtgaccagt tttatcaacg acgccttttc ttttgagttc ccacagctcg
     7741 ttgacagcct cttcacgact gattccaagg cgagctgcca ccacatgtga agaggctttt
     7801 ttcagtgctt tcagtgcgtc agatacggtt tccattaaaa tttcctccgg acaaaattac
     7861 ttcacaaccc tcatattgct gacatttgga cgccagctat cccagttaaa cgtcacccat
     7921 cgaccaccgt tcatggtcat gcggtccata atcctctcac caagaagcgt actcattgcg
     7981 gcatgattca ggtttgttaa catcccgaca ctgcacagtg atgctgtccg gcgatcaatt
     8041 atctggtgca ataccacctg ctcgtttttc gtctcccgct gaacgcctat ttcatccagg
     8101 accagcaaat caaccccgca aagctcctgt aaaaattttt ccccggattt gccgttgtcg
     8161 tagctgtcat gcaacacgct catgacgtca gacacggtga cgataatcac gctgcgcccc
     8221 ttcaccatca gccggttgcc catcgccgct gcaaggtgat ttttcccggt gccggtttta
     8281 ccgctgaaca caaaattcgt gcacccggtc atcagttcgt cagctatgga tttggcctgg
     8341 ctcagcgcgt atttttgccc gtcgttctgc acctgataat ttgcaaacga gcatttgctg
     8401 tgcagaggct ggatgcccga acgattcagg attttttcca cccgcaactg gcgattctgg
     8461 cggttaatct cctcgctgcg ttttcgtcct tcagcaagtt gccattcccg ccactcctcc
     8521 accgtccggt acggtggaac cgacccctgt ggtgcaagtc tgcgaatacg ttcaagaacc
     8581 ccaactgccg caatgttttt catgacacgt caccccctga atcccggcgg tatttcagtg
     8641 tccggttcag aaatgtgatt cacgcaacgc tgcgcaggcg aacgccccag gcggataacc
     8701 agttcatccc atttttcccg gagttttgcc ggactcatga tgttttttac ccagaacgaa
     8761 tcccgctgga gacgcccaaa catttcacaa atttgtctgt gagttctgcc atccagcatc
     8821 cgcattgtgc gaacgtcatt ggcccatgct gtccagttgg gttctttcgg tctagtgatc
     8881 tcgccatcat agctggccgc ctgctcgtaa agactcacga ttcgtcccca gatccactgt
     8941 gcgcacacca aatcttcctg acttccccac tggcgttttt tcgcactgaa cacaaccgcg
     9001 tcagggtgtc gggttaaaaa atcctgttca gccgtctgcg ggtccggttg cgaagcgtcc
     9061 ggacaagaag atcttttatc tgacggatca ggttttaata ctgacggatc ggggtcaatc
     9121 atcggccccc taatcg
//
LOCUS       DAOFSV010000166         9089 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.166, whole genome shotgun sequence.
ACCESSION   DAOFSV010000166 DAOFSV010000000
VERSION     DAOFSV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.166"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(7..1383)
                     /gene="trkA"
                     /locus_tag="QBH27_004106"
     CDS             complement(7..1383)
                     /gene="trkA"
                     /locus_tag="QBH27_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDQ7016355.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1405..2694)
                     /gene="rsmB"
                     /locus_tag="QBH27_004107"
     CDS             complement(1405..2694)
                     /gene="rsmB"
                     /locus_tag="QBH27_004107"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDQ7016356.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRNPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(2746..3693)
                     /gene="fmt"
                     /locus_tag="QBH27_004108"
     CDS             complement(2746..3693)
                     /gene="fmt"
                     /locus_tag="QBH27_004108"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDQ7016357.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(3708..4217)
                     /gene="def"
                     /locus_tag="QBH27_004109"
     CDS             complement(3708..4217)
                     /gene="def"
                     /locus_tag="QBH27_004109"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDQ7016358.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4347..5471
                     /gene="dprA"
                     /locus_tag="QBH27_004110"
     CDS             4347..5471
                     /gene="dprA"
                     /locus_tag="QBH27_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDQ7016359.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5443..5916
                     /locus_tag="QBH27_004111"
     CDS             5443..5916
                     /locus_tag="QBH27_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDQ7016360.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5945..6487
                     /locus_tag="QBH27_004112"
     CDS             5945..6487
                     /locus_tag="QBH27_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDQ7016361.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACGYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            6492..7064
                     /gene="tsaC"
                     /locus_tag="QBH27_004113"
     CDS             6492..7064
                     /gene="tsaC"
                     /locus_tag="QBH27_004113"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDQ7016362.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7069..7887
                     /gene="aroE"
                     /locus_tag="QBH27_004114"
     CDS             7069..7887
                     /gene="aroE"
                     /locus_tag="QBH27_004114"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDQ7016363.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLTPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMHELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            7884..8141
                     /locus_tag="QBH27_004115"
     CDS             7884..8141
                     /locus_tag="QBH27_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDQ7016364.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLESFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8117..8671)
                     /locus_tag="QBH27_004116"
     CDS             complement(8117..8671)
                     /locus_tag="QBH27_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDQ7016365.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTIGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         2291 a         2204 c         2249 g         2345 t
ORIGIN      
        1 tattaattac aagaagaaag gacttggctg gaagagtctt tcgacgtcgg taataaactt
       61 tttatctgtg aggaacataa ttacgtgatc gccttgctca atgcgcagat tgtcattggc
      121 aatcatcaca tcgtttccac gtaccaccgc tccaataatc gttcctggcg gtagcttgat
      181 ttcgtcaatg actctgccga caacgcgtga ggtgctttca tcaccgtgag caacggcttc
      241 aatagcttct gctacgccgc ggcgtaatga ggaaacacca acaatatctg cttttcgcac
      301 atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa atcgcaatat cgatcacgct
      361 cccctgaacc agatccacat aagcgcgacg ttggatcaat accatcacct ttttcgcacc
      421 catacgtttg gcaagcatgg cggacatgat attggcctcg tcatcgttgg tgacggcaat
      481 aaacagatca acttgatcga tatgttcttc ggccagcagt tcttgatccg acgcatcacc
      541 aaaaaagacg atcgtattct gcaacttttc cgccagttcg gcagcgcgct gctgattacg
      601 ttcgatgagt ttaacgctgt aatctttttc cagacgacgc gccagccctg caccgatatt
      661 accgccacca accagcatga tccgcttata cggtttttcc agtcgctgta attcactcat
      721 caccgcacgg atatgctgtg aagcggcaat aaagaacact tcatcaccag cttcaacaat
      781 ggtcgaacct tgcggacgaa tggggcgatc gtggcggaaa attgctgcca cacgagtatc
      841 gatatgtggc atatgttcgc gcatggtcga aagtgcatta ccaatcagcg ggccgccata
      901 ataggcttta accacagcca ggctgacttt accctcagcg aagttcacca cctgcaatgc
      961 gccgggatac tcaatcagtc ggtaaatatt atcgataacc aactgctctg gtgcgatcag
     1021 atgatcaatc ggtacagcat ctgaatgaaa tagtttatcg gcatcgcgca cgtagtctgg
     1081 tgagcggata cgagcgatgc gattaggggt gttgaaaagt gagtaggcta cctggcaggc
     1141 aaccatattg gtttcatctg aactggttac agcaaccagc atatcggcgt cgtcggcacc
     1201 tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc acgacccgca ggtcaaattt
     1261 atcctgtaag gtccgcagac gctcaccgtt ggtatcgaca acagtaatat cgttgttctc
     1321 gccaaccagg ttttccgcca gtgtgccgcc aacctggccg gcacccagaa tgataatttt
     1381 catcagtcgc gacccgttat ctcatcactt tttgattagc ttagcgtaaa agaagccgtc
     1441 gccctcttcg gcaccgggca aattttgttt acccggttgc tctggtgttc ctgtttcaca
     1501 aagttcggca tcagcggtac gttgcaaaaa ggctttaatc tgcaggctat tttcttccgg
     1561 taacaccgaa caggtggcat agaccagggt tccaccggat tttaaatgtg gccaaatggc
     1621 atcgagtatt tcagactgca attgcgcgag ttccgggata tcgcgatcac gacgtaacca
     1681 tttaatatcc ggatggcgac gaatcacccc cgttgctgaa caaggcgcat ctaataaaat
     1741 gcgatcaaac tgttgctcgc cacaccattg ggaagggttg cggccatcac cttgtttcac
     1801 ggtcgccttc ataccaaggc gttttaaatt gtcgtaaacg cgagagaggc gctgctcatc
     1861 aatatcgacc gccagaactt gcgcctctgg agcaacctca agaatatgcg ttgttttacc
     1921 gcctggggcg gcgcaaagat ccagaatgtg ttcaccattt tgtggagcaa gccaggtcat
     1981 gcaaccttgt gctgaagcat cctgaacggt aacccatcct tcttcaaaac cgggtagcgc
     2041 atgaacaggt gcaggtgttt ccagctgtac agcatcaggg taatccgcat gcgggaaacc
     2101 tttcattcct gcttcatcca gcaatgcaag ccagctgtcg cgggaatgat gcgtacgatt
     2161 aacgcgcagc cacattggcg gacgctggtt attggcttcg acgatggatt gccactgctc
     2221 gggatacgcc ttttgcagac gcttcagcaa ccaggaagga tgcagataac gtgcatcact
     2281 ggcattaaac tcggctaata actcatcttg ctgacgctgg aactgacgta atacaccgtt
     2341 aatcaatccc ttaagttgcg ggcgcttaat tgcaactgcg ccttcaaccg tttcagccag
     2401 cgcggcatga ggtggaatgc gggtataaag cagttgatac aaaccaacca taatcaggta
     2461 atgaacagta cgctgtttgc ctgtcatcgg acgggccatt agcttattaa tcagccagtc
     2521 taactgtgag agcgtacgca gtacgccaaa gcataactcc tggagaagag ctttatcttt
     2581 atcggaaact ttttgctgga gcggtggcag aatgttgctc agtgattgcc cttgctcaac
     2641 gacttgttca atggcctggg ccgccatgct acgtaaatta cgttgttttt tcataagtat
     2701 aaatataaag atgcccggta agaccgggct tagaagagtg gactatcagg ccagacggtt
     2761 gcccggtaca aaccattccc gacgagaatt caggaggtct tgcacgctca tcgctttctt
     2821 acctgcaggt tgcattgaga gcagattcag gatgccatca cctgtcgcta cctgaatgcc
     2881 ttgtttattg gcttcaagga ttgttcctgg cgcagccttt gttgtcgtat cgatgacaga
     2941 tgctttccag actttaaccg gctgcccttc aatttccagc cagctcattg gccatggatt
     3001 gaaagcgcga atgcaacgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc
     3061 ttctttactc aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc
     3121 cgtgccgtct gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag
     3181 cttgtcgtac agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag
     3241 catgtcaccg gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc
     3301 acccgcccat agtgagcgtt ggattggtgc tgcaccgcgc cagcgtggca gcagtgaacc
     3361 atgaacgttg atacagccaa gacgcggcat ctccagcaca gctttaggca gaattaaacc
     3421 ataggcgacg acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg
     3481 tggacgcagg gaaacaggtt gaaaaacggg cagacctttg tcctcagcca gaactttaac
     3541 cgggctgggc atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc
     3601 aacgatgtta tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt
     3661 acccgcaaaa ataatacgta gtgattctga cacgttaatt cttatcctta agcccgggct
     3721 ttcagacgat ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga
     3781 taatccataa acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac
     3841 agaccgtctg cttccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact
     3901 ttctctgcgc gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg
     3961 cctgtttcgc cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg
     4021 ttttccgaaa catcaattac aatgatacgt tgatggatat caacctgggt tgccgccagg
     4081 ccaatacctt cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct
     4141 gcattcactt cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac
     4201 acttgcaaaa ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt
     4261 ctattctaga caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta
     4321 aggctgctta tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga
     4381 gcatcagcag cttgtatggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt
     4441 cgcatattga tgcggttgta ttgcagcaaa cagggcttac attgcggcag gcacaacgct
     4501 ttctttcatt tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc
     4561 atcatttaat ccctgcggac agcgaatttt atcctcctca acttctggcg acgacagatt
     4621 accccggcgc actgtttgtt gaaggagaac tgcacgcgtt gcattcattt cagcttgccg
     4681 tagtggggag tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa
     4741 ctctggcgac gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg
     4801 cgcataaagc ggccttacag gtaaatggcg ttagcattgc tgtattgggg aatggactta
     4861 ataccattca tccccgccgc catgctccac tggctgccag tctacttgag caaggtggtg
     4921 ctctcgtctc ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa
     4981 atcgcattat cagtggtcta agtaagggtg tactggtggt ggaagcggct ttgcgcagtg
     5041 gttcgctggt gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gctttgccag
     5101 gtccaatagg gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc
     5161 ttgtgacgga accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag
     5221 acgcccctga aaattcattt tattcaccag atcaggaaga cgtggcattg ccatttcctg
     5281 agctcctggc taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg
     5341 gccaacctgt gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg
     5401 cagctgtacc cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg
     5461 tatttgtttg aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt
     5521 gaacaggatc ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg
     5581 ctggaaaagc ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat
     5641 ccactctcca tgcgtattta tacccctgaa gagtgtgaaa gactggatgc cagctgtcgt
     5701 ggttttttgc ttttccttga gcaaattcag gtgcttagcc ttgaaacccg tgaaatggtg
     5761 atagagcgag tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta
     5821 atcctgatgg tgttgttcaa tattccgggt tgcgaaaatg cgtaccagca aatggaagaa
     5881 ttactctttg aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt
     5941 tgttatggcg aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg
     6001 ggctgaacta gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc
     6061 ggcatgtggc tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct
     6121 ggaggggcag gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg
     6181 tatgtttatt ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc
     6241 ggacgaaaca gcaattacat gcccccaatg ccggacgggc catctggttc agcgccgctc
     6301 ccgttatggc aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa
     6361 cttcaaaccc atagctggag aatgccctga gtgccattat ccgctactca tcgaaaagaa
     6421 aaccgcgcag ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cgatttcggc
     6481 ggaataataa cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc
     6541 tcaatgaaga acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc
     6601 ctgatagcga aacagcagtg atgagactgt tggagttaaa acggcgtccg gttgataagg
     6661 ggctgatttt aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc
     6721 tgactgacgc gcagcgtgaa accatttttt cccgctggcc aggtcctgta acctttgtct
     6781 ttcccgcgcc tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac
     6841 gagtcaccga ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt
     6901 ctaccagtgc caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac
     6961 aatttggcgc tgcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag
     7021 aaatccgcga tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat
     7081 gctgtttttg gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct
     7141 cagcaactga atattgaaca tccctatggg cgtgtgttga cacccatcaa tgatttcatc
     7201 aacacactga acactttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt
     7261 aaagaagagg cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct
     7321 gttaataccc tcaagcggtt agaagatgga cgcttgctgg gtgacaatac cgatggtata
     7381 ggtttgttaa gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattttgctc
     7441 atcggcgctg gtggagcatc tcgcggcgta ctactgccac tcctttccct ggactgtgcg
     7501 gtgacaatca ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac
     7561 actggcagta ttcacgcgtt gggtatgcac gaactggaag gtcatgagtt tgatctcatt
     7621 attaatgcaa catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt
     7681 catccaggca tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca
     7741 tggtgtgagc agcgaggctc aaagcgtact gctgatggtt taggaatgct ggtggcacaa
     7801 gcggctcatg ccttccttct ttggcatggc gttctgcctg atgtagaacc ggtcatcaaa
     7861 ctattgcaac aggagttgtc agcgtgaatc aggccatcca gtttccggat agggaagagt
     7921 gggacgagaa taaaaaatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat
     7981 gtgcgatctc tggcgagagt ctggcgtatc gctttactgg agatacacca gaacagtggt
     8041 tagagagttt tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg
     8101 aacaaagtga agatgatcaa ggttgggtgt ggttaccctg atccatatat tcgtccttcc
     8161 atttcacgta gttatttgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc
     8221 ggatctgttt gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg
     8281 ggaccaggct acctgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa
     8341 ttgagcccat cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt
     8401 gaccaatagt gacatcttcg ccaatggtta atgggttgcc agctgggttg tacgaggatt
     8461 tatgagtgac gtgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat
     8521 aatgtacatc tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa
     8581 cgtcaccaat cacgacgctg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa
     8641 gatcgcggta tgggcgtaaa acctcagaca tactcacctc aacaataaat gatttactaa
     8701 tgactttggg tacattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt
     8761 tgtgtgcgaa tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag
     8821 tacttgtgca aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac
     8881 acttcacaag atagtcgtaa caacaaagag aaaaatcctg aaattcaggg ttgactctga
     8941 aagaggaaag cgtaatatac gccacctcgc gacagtgagc tgaaaaccgc gtcgcaactg
     9001 ctctttaaca atttatcaga caatctgtgt gggcactcga agatacggat tcttgacgtc
     9061 gcaagacgaa aaatgaatac caagtctca
//
LOCUS       DAOFSV010000167         9078 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.167, whole genome shotgun sequence.
ACCESSION   DAOFSV010000167 DAOFSV010000000
VERSION     DAOFSV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.167"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(31..558)
                     /gene="mobB"
                     /locus_tag="QBH27_004117"
     CDS             complement(31..558)
                     /gene="mobB"
                     /locus_tag="QBH27_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDQ7016366.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(540..1124)
                     /gene="mobA"
                     /locus_tag="QBH27_004118"
     CDS             complement(540..1124)
                     /gene="mobA"
                     /locus_tag="QBH27_004118"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDQ7016367.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1194..1463
                     /locus_tag="QBH27_004119"
     CDS             1194..1463
                     /locus_tag="QBH27_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDQ7016368.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1540..2526
                     /gene="srkA"
                     /locus_tag="QBH27_004120"
     CDS             1540..2526
                     /gene="srkA"
                     /locus_tag="QBH27_004120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDQ7016369.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2543..3169
                     /gene="dsbA"
                     /locus_tag="QBH27_004121"
     CDS             2543..3169
                     /gene="dsbA"
                     /locus_tag="QBH27_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDQ7016370.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            3323..4753
                     /locus_tag="QBH27_004122"
                     /pseudo
     CDS             3323..4753
                     /locus_tag="QBH27_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(4794..5726)
                     /locus_tag="QBH27_004123"
     CDS             complement(4794..5726)
                     /locus_tag="QBH27_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDQ7016371.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVRGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            6084..8870
                     /gene="polA"
                     /locus_tag="QBH27_004124"
     CDS             6084..8870
                     /gene="polA"
                     /locus_tag="QBH27_004124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDQ7016372.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         2531 a         2089 c         2200 g         2258 t
ORIGIN      
        1 gctggcgcgt ttgcttcagc ctattcttgc ttatccgtcc tgcttttgca tccactccac
       61 cacaaaatca gccagcccct caacatcatt aatatccagt aacgcgacat caagattaag
      121 cggcacatca ctggctacag caataacatg cctgtctatc actaattctt ccggtcgatg
      181 tccggctcca tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      241 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaaatgta gatccagctc
      301 ttcttcgtct ggtgtttccg tcatcaaggc ccatcgctgc tggctggcaa cgatggtttg
      361 cgccgcgcca gccttgcgca gctcatagct atctttgccc ggtttatcaa catccatatc
      421 atggtgcgta tgcttaatca gccccggacg gatccctctg gcgcataatg ccgggatcaa
      481 ttttttcagc agcgttgttt ttccggtgcc actccatgca gcaaaggcga gtaacggtat
      541 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgtttat aaatgcctct
      601 ttacgatcgc tgaaatcaac cgcatgaccg ccagccagac gcataaatgc cataacccgg
      661 cgttctcctg cttgcagata ttccagtaat aaaggctcaa ttgcgcggtt taccagagca
      721 atagtcgggt gatcgcgttc accgtcgtgg acccacacga caggcgcatc tttgcgctga
      781 tgagtaagcc gggcggctaa atcatgggga atgtaaggcg tatcgcacgg gcaaaacaaa
      841 aaccactcac ccgcttcctg ctgcattact gaaagcattc ctgccagagg gccagggtaa
      901 tccgccagtg aatcttcaat cactttcaga ccgcttacct gatagatttc ctgatgacga
      961 ttagcattaa ccacgacgtg agaaagctgc gtcataagcg cgtcagcgac atgttgccat
     1021 aatggtttgc cgtttaattc aagcaatcct ttatctacgc cgcccattcg tctggcttta
     1081 ccgcccgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1141 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatt actatgaaat
     1201 gtaaacgtct gaatgaagtt attgaactcc tccagccagc ctggcaaaaa gagccagacc
     1261 ttaacctgct gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1321 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1381 cggtgatccc gggtttgcag aaagattatg aagaagattt caaaacggcg ctattacgcg
     1441 cacgtggcgt aattaaagag taaaagcttg taagcggcgc aaccaaaatc atcgtgaaat
     1501 gatatccttc atcattcgta atgttttccg gatgatggga tgaacaacag cgcttttact
     1561 ttccagacac tacacccgga taccatcatg gacgctctgt ttgagcatgg gatccgggtg
     1621 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1681 gatcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1741 ctcgaagaac atcaatttgc gttgcagctg gttaacgatg aagttccggt cgcagcacct
     1801 gtggccttta atggtcagac tttattgaac catcagggat tttatttcgc tgtttttcca
     1861 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agatggaagc ggttgggcgt
     1921 tatttagggc gtatgcacca gacggggcgc aaacagcttt ttatccatcg cccgaccatc
     1981 ggtttgaatg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2041 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgattgccgc cgttacagca
     2101 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2161 ctctggcgcg atggcccaat gtttgttgat ctggatgatg cacgtaatgg tccagccgtt
     2221 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2281 attattgagg cttatgaaga atttagcgag ttcgacaccg ctgaaatcgg actgattgaa
     2341 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2401 cccgcgttcc cgaaaaattt cccgtggtta accggggaag attactggct acgacagacg
     2461 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttgcaatt aacacctatg
     2521 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2581 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2641 accagttgct ggcgcgccgc aagtgctgga gtttttctct ttcttctgcc cgcactgcta
     2701 tcagtttgaa gaagttctgc atatttctga taacgtgaag aaaaaactgc cggaaggcgt
     2761 gaagatgact aaataccacg tcaacttcat ggggggtgac ctgggcaaag agctgactca
     2821 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtcacagttc cgctgtttga
     2881 aggcgtacaa aaaacccaga ccattcgttc agcatctgat atccgcgatg tatttatcaa
     2941 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tgaaatctct
     3001 ggtcgctcag caggaaaaag ccgcagctga cgtgcaattg cgtggtgttc cggcgatgtt
     3061 tgttaacggt aaatatcagc tgaatccgca gggtatggat accagtaata tggatgtttt
     3121 tgttcagcag tatgctgata ctgtgaaata tctgtccgag aaaaaataat tcaatgtaaa
     3181 taacataaag cccgtgaata ttcatgggct tttttattat ttaataaata taaatacatt
     3241 ctgataatgc gtcctgctgc tggacattat ttcagcataa gtggatattc aaagttttgc
     3301 tgttttatca gggaatattt atatgatacg taagtcagct acaggtgcca ttgttgcgtt
     3361 atccgtaatc tggggtggtg gcacatggta cacaggtacg caaattcagc caggtgtcga
     3421 aaagtttatt aaagatttta acgatgctaa atagaaaggt gaacatgcct acgacatgac
     3481 gttaagttat aaaaattttg acaaaggttt ttttaattcc cgttttcaaa tgcaaatgac
     3541 atttgataac ggtgcacccg atctcaatat caagccaggc cagaaagttg tatttgatgt
     3601 ggatgttgag cacggtccgt tgcccatcac aatgttaatg catggtaatg tcatcccagc
     3661 actggcagcg gcaaaagtga acttagtgaa taatgagctg acacaaccgc tatttatcgc
     3721 cgcgaaaaat aaatcgcccg tggaagcgac attgcgattc gcgtttggtg gctcattctc
     3781 cacgacatta gatgttgccc ctgcagagta tggaaagttt tcttttggtg aaggccagtt
     3841 tacttttaat ggtgatgata gttcattgtc taacctggat attgaaggca aagttgaaga
     3901 tattgttctg caattatcac caatgaacaa agtaacggcg aaaagtttta ccattgattc
     3961 tctggcgcga ttagaagaaa agaaatttcc ggttggtgaa agcgagtcga aatttaatca
     4021 gattaacatt atcaatcacg gggaagacgt tgcccaaatc gatgctttcg ttgcaaaaac
     4081 caagctggat cgcgttaaag acaaagatta tatcaatgtc aatctgacct acgaacttga
     4141 taagttaaca aaagggaatc agcaactcgg tagtggtgag tggtcattga ttgctgaatc
     4201 tattgatccc tcagcggtgc gccaatttat catccagtat aacattgcga tgcagaagca
     4261 gcttgctgca caccctgagt tagcaaacga tgaagttgct ctgcaagaag tgaatactgc
     4321 attgttcaaa gagtatttac cgttattaca aaaaagtgag ccgaccatta aacaaccggt
     4381 aagatggaag aatgcactcg gcgaactaaa tgccaatctg gatatcagta ttgccgaccc
     4441 agccaaatct tcatcatcca caaacaaaga tattaaatcg ctcaattttg atgtgaaatt
     4501 accgcttaat gtcgccacag aaaccgcaaa acagcttaat ttatctgaag gaatggatgc
     4561 ggaaaaagcg caaaagcagt ctgataaaca aatcagcggg atgatgacct taggtcagat
     4621 gttgcagtta atcacgattg acaacaatac cgcctcgctg caactgcgtt atacaccggg
     4681 taaagttgtt tttaacggac aggagatgag cgaagaagaa tttatgtctc gtgccggacg
     4741 ttttgttcat taaaatactc actcgcctct gctcattcag aggcgagcaa aatttacttg
     4801 tcctgccgct gtgatgacat caacgacgtc agaagccggt ctttttcctg ccacagtgag
     4861 ttcagccatt gctgaaaatg gcgcttaaag cttttgtcat tgatgtagtc gccatgtaac
     4921 tcatcggcaa taggctgcaa atccacatgt acgacaattc gcgtcagttt accgcttaac
     4981 atatcgaaga acggctgacg gttattgtcc ggataacaca gcgtaacatt aagcagttta
     5041 tcgaattgtt tacccagtac atttagcgcc atcgcaatgc ctgccgcctt tggtggcaac
     5101 aagttttgaa aagtggagtg cgtttgctga tgtttttctt gcgtgaaacg ggagccttca
     5161 acgaaattaa caatggtggt gggatgcagg cgaaactttt cacaagaacg ccgagtggtt
     5221 tcaacatctt taccacgtct ctccggatga cgtaataaat aagcgcggga ataacgcttc
     5281 ataaacggca tatccagcgc ccagcatgcc aggccaagaa aaggcaccca ggccagctgc
     5341 tgcttgagga aatatttatt catcggaatg tgcttacgaa acagcacgca cagtacgaca
     5401 atatctgccc aactacggtg gttacaaata agcagatacc agtttttctt acttaaccct
     5461 tccagcccgc gaacttccca ttgcaggtgt gggttcagat gcagtaatac cgccagacct
     5521 tcacaccagc aatacatcat aaaatcacaa aaacgcgaaa cctttcgcca aatgactggc
     5581 acaggaagca atagttttac aatcccggca atgatgatcg ggacggagca aaaaatagtg
     5641 accaaaatgg tcaatacgat actcagcaaa agggttatcg cagcgagtat tctcgtcata
     5701 atgaatttat tcaaaaggtt agccatagac agtgcgctaa agaagcgcaa ggcgcagatt
     5761 ttagcagaaa acgacccaaa taacggatga tccttaagga gaaaaataat tcatatctat
     5821 ccacattaga aaaaatccca ttatctcaag ggatggcttt atttttaact gcatgaaaat
     5881 caaagacaaa catcatgctg taaaaagcat gataataaat taaaagcgat gtaaataatt
     5941 tatgcacaaa gttatccaca tgacgatttg cgagcgatcc agaagatcta caaaagattt
     6001 tcacgaaaag cggtgaaaaa ctcatgtttt catcctgtct gtggcatcct ttacccataa
     6061 tctgataaac aggcacggac attatggttc agatccccca aaatccactt atccttgtag
     6121 atggttcatc ttatctttat cgcgcatatc acgcgtttcc cccgctgact aacagcgcag
     6181 gcgagccgac cggtgcgatg tatggtgtcc tcaacatgct gcgcagtctg atcatgcaat
     6241 ataaaccgac gcatgcagcg gtggtctttg acgccaaggg aaaaaccttt cgtgatgaac
     6301 tgtttgaaca ttacaaatca catcgcccgc caatgccgga cgatctgcga gcacaaatcg
     6361 aacctctgca cgcgatggtt aaagcgatgg gactgccgct gctggcggtt tctggcgtag
     6421 aagcggacga cgttatcggt actctggcgc gcgaagccga aaaagccggg cgtccggtgc
     6481 tgatcagcac tggcgataaa gatatggcgc agctggtgac gccaaatatt acgcttatca
     6541 acaccatgac gaataccatc ctcggaccgg aagaggtggt gaataagtac ggcgtgccgc
     6601 cagaactgat catcgatttc ctggcgctga tgggtgactc ctccgataac atccctggcg
     6661 taccgggcgt cggtgaaaaa accgcgcagg cattgctgca aggtcttggc gggctggata
     6721 cgctgtatgc cgagccagaa aaaattgctg ggttgagctt ccgtggcgcg aaaacaatgg
     6781 cagccaagct cgagcaaaac aaagaagttg cttatctctc ataccagctg gcgacgatta
     6841 aaaccgacgt tgaactggag ctgacctgtg aacaactgga agtgcagcca ccggcggcgg
     6901 aagagttgtt agggctgttc aaaaagtatg agttcaaacg ctggactgct gatgtcgaag
     6961 cgggcaaatg gctacaggct aaaggggcga aacccgcagc caggccgcag gaaaccagtg
     7021 ttgcagacga agcgccagaa gtgacggcaa cggtgatttc ttatgacaac tacgtcacca
     7081 tccttgatga agaaacactg aaagcgtgga ttgcgaagct ggaaaaagcg ccggtatttg
     7141 catttgatac cgaaaccgac agccttgata acatctctgc taacctggtc gggctttctt
     7201 ttgctatcga gccaggcgta gcggcatata ttccggttgc tcatgattat cttgatgcgc
     7261 ccgatcaaat ctctcgcgag cgtgcactcg agttgctaaa accgctgctg gaagatgaaa
     7321 aggcgctgaa ggtcgggcaa aacctgaaat acgatcgcgg tattctggcg aattacggca
     7381 ttgagctgcg tgggattgcg tttgatacca tgctggagtc ctacattctc aatagcgttg
     7441 ccgggcgtca cgatatggac agcctcgcgg aacgttggtt gaagcacaaa accatcactt
     7501 ttgaagagat tgctggtaaa ggaaaaaatc aactgacctt taaccagatt gccctcgaag
     7561 aagccggacg ttacgccgcc gaagatgcag atgtcacctt gcagttgcat ctgaaaatgt
     7621 ggccggatct gcaaaaacac aaagggccgt tgaacgtctt cgagaatatc gaaatgccgc
     7681 tggtgccggt gctttcacgc attgaacgta acggtgtgaa gatcgatccg aaagtgctgc
     7741 acaatcactc tgaagagctc accttgcgtc tggctgagct ggaaaagaaa gcgcatgaaa
     7801 ttgcaggtga agaatttaac ctttcttcca ccaagcagtt acaaaccatt ctgtttgaaa
     7861 aacagggcat taaaccgctg aagaaaactc cgggtggcgc gccgtcaacg tcggaagagg
     7921 tactggaaga actggcgctg gactatccgt tgccaaaagt gattctggaa tatcgtggtc
     7981 tggcgaagct gaaatcgacc tacaccgaca agctgccgtt gatgatcaac ccgaaaaccg
     8041 ggcgtgtaca tacctcttat caccaggcag taaccgcaac gggacgttta tcgtcaaccg
     8101 atcctaacct gcaaaacatt ccggtgcgta acgaagaagg tcgccgtatc cgccaggcgt
     8161 ttattgcgcc agaggattat gtgattgtct cagcggacta ctcgcagatt gaactgcgca
     8221 ttatggcgca tctctcgcgt gacaaaggct tgctgaccgc attcgcggaa ggaaaagata
     8281 tccaccgtgc aacggcggca gaagtgtttg gtttgccact ggaaaccgtc accagcgagc
     8341 aacgtcgtag cgcgaaagcg atcaactttg gtctgattta tggcatgagt gctttcggtc
     8401 tggcgcggca attgaacatt ccacgtaaag aagcgcagaa gtacatggac ctttacttcg
     8461 aacgctaccc tggcgtgctg gagtatatgg aacgcacccg tgctcaggcg aaagagcagg
     8521 gctacgttga aacgctggac ggacgccgtc tgtatctgcc ggatatcaaa tccagcaatg
     8581 gggctcgtcg tgcagcggct gaacgtgcag ccattaacgc gccaatgcag ggaaccgccg
     8641 ccgacattat caaacgggcg atgattgccg ttgatgcgtg gctacaggct gagcaaccgc
     8701 gtgtgcgtat gatcatgcag gtacacgatg aactggtatt tgaagtccat aaagatgatg
     8761 tcgatgccgt cgcgaagcag attcatcaac tgatggaaaa ctgtacccgt ctggatgtgc
     8821 cgttgctggt ggaagtgggg agtggcgaaa actgggatca ggcgcactaa gattcgcctg
     8881 aacatgccct tttttcgtaa gtaagcaaca taagctgtca cgttttgtga tggctattag
     8941 aaattcctat gcaacaactg aaaaaaaatt acaaaaaatg ctttctgaac tgaacaaaaa
     9001 agagtaaagt tagtcgcgta gggtacagag gtaagatgtt ctatctttca gaccttttac
     9061 ttcacgtaat cggatttg
//
LOCUS       DAOFSV010000168         8930 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.168, whole genome shotgun sequence.
ACCESSION   DAOFSV010000168 DAOFSV010000000
VERSION     DAOFSV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.168"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..385)
                     /locus_tag="QBH27_004125"
     CDS             complement(<1..385)
                     /locus_tag="QBH27_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDQ7016373.1"
                     /translation="MYSPYLYARSTELLCLRDLVAKGVSLNGLLPILEPINVNTRNLL
                     TCLNVWNSDIIVILNPYQEDFSSHNNVLTLNQNLSSVLTNKNNIISGYLIQPNTTIND
                     INNWLNANLNRRVALLYDNPSLSNSD"
     gene            complement(407..1288)
                     /locus_tag="QBH27_004126"
     CDS             complement(407..1288)
                     /locus_tag="QBH27_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDQ7016374.1"
                     /translation="MSHNKKEYAKFFRRPIFSSLAKGKDISDLFGSFDSYCYETLFSN
                     NDNNEHEISLRDLFTSLYDFLRLNYRNEYVYKTALVNKIIFGKHSPKTSSSSIELPIK
                     NSIVDVAVFNGTSTAYEIKTEYDSPKRLITQAPDYLDVFDKVYIVTHPEYASKYCALN
                     LPRVGVMVLNKKDQLSVIKEADSNIDYIKSDSLFSVLRKEEFLAIIEDYTSTKINMPN
                     GLVYEYCKEIFMQMPLNIANKYFISAMKKRCNDKVFLDYIYSLPACLRVLGYATPLSR
                     KQKEFIMNLMDVKIKIC"
     gene            complement(1968..2240)
                     /locus_tag="QBH27_004127"
     CDS             complement(1968..2240)
                     /locus_tag="QBH27_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDQ7016375.1"
                     /translation="MKMMRMYCPTCQAVARIGKTNRKHPQLYDVYCYCSNVECGHSFV
                     MNVAFSHSVSPSALNGQGRVKELIDAIPPEEREKALKLLLAAQKNG"
     gene            complement(2523..5210)
                     /locus_tag="QBH27_004128"
     CDS             complement(2523..5210)
                     /locus_tag="QBH27_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDQ7016376.1"
                     /translation="MKTPSVSTISGAAIGRWPYILSALGIKVPSAGHHGACPACGGKD
                     RFRLDDKAGRGTWFCNQCGHGDGLDLVRLVTGRKIKEVAGMVSEALALPEIQEKPALP
                     ARKKAAGKEAGAERYTRLRQQSCNGEPVYLTNKSLHGYSLPLLSQPLNLAGITFSTGS
                     LLLPLTDISGNITGGQLINPDGDKSLLPGSQLSGAFIALTDIPAETPEQVIITEGFAT
                     ALTVSLLTEGWIVAAVAATNLLKVTEQIRKRWPETRIILAGDNDLADGKENTGRIQAE
                     KAAKAVDGWVTLPPVRHKADWDDYRQEVGKERARDAFREEMTLHGKGQTRLPEGFRLT
                     KEYLWYDKLVNKSDGDTEIRNIKICSPLRVTAITSDADGSNYGRLLEWEDTNGNSRKW
                     AMPMEMLGGSGEELRRVLLVNGLSYININGMARAFLMEYISLCKPDRKVTCVNKTGWH
                     GGVYVLQDEVIGREAQSVILQTSSVQGRDFRVSGTSEGWRENIGRYCIKNARLAFAVS
                     LAFAAPLLKLVGIGGGGYHLKGESTDGKTTTMKVAASVCGGTDFWHTWRATGNALEGT
                     ASRRNDATLMLDEIREVDGREAGNIAYMLANGQGKARARTDGSVRETNRWNLLFLSTG
                     ELSLVEHAASAGERTYAGVEVRMIQIPSDSGKYGVFEELHGFSSGKTLAEHLEQHVAH
                     YHGAPFRDWLYCLTADLPELTSQAKALLKEYTRRLTPENAGNQVGRAVTRFALVAMAG
                     ELATKAGITGWPEGEAFRAAQSCLAAWMADRGHTANQEDKAALEQVRDFMTRNQFSRF
                     ADWNDDRNRPVSMMGFRKVDKGDNVTEPVVTFYVLPSGWKEICKGFDSRKVARLCVDA
                     GWLKPGEDGRTQNSIRLPEIGLKRVYQFNTQVLGSAEPE"
     gene            complement(5207..5617)
                     /locus_tag="QBH27_004129"
     CDS             complement(5207..5617)
                     /locus_tag="QBH27_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016377.1"
                     /translation="MNNNQNLKAHALRTAVLRYYIADAFISLMVRVHNEPVYIEDGER
                     IELSHEKVVSNIIYHIEMPWVNEFGADAGCILAAEKLEKMLKPGFMSENIRLSVFGVT
                     EMREVYRDIIFGAPDGELPEGFEFVKPEGQEVLL"
     gene            complement(5610..5846)
                     /locus_tag="QBH27_004130"
     CDS             complement(5610..5846)
                     /locus_tag="QBH27_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016378.1"
                     /translation="MKSMNFKQGCILSEQGESRLLRAALASEFLAEVLSVPTVNGART
                     VSAEGAAALVACIAEQLDGVVKETSTIKGVMRDE"
     gene            complement(5843..6433)
                     /locus_tag="QBH27_004131"
     CDS             complement(5843..6433)
                     /locus_tag="QBH27_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDQ7016379.1"
                     /translation="MPALINPGNDQFSVINFLPHQECAGYSLKVAAKSAAGRRNPCYL
                     KAILHALGVFFCVAALTHLKFMVWWLIVFFYFLRHIIRIMVVQAGQLSGWPVSFRAGI
                     PTPVWATTHERRNSGGSVTRYLKEVAIMATVPALSHPEFTFVFLAVRRTDRDARPRPV
                     RVIAECEHAARLKLATEFILSFAARIPVKRAGEVVA"
     gene            complement(6521..7525)
                     /locus_tag="QBH27_004132"
     CDS             complement(6521..7525)
                     /locus_tag="QBH27_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDQ7016380.1"
                     /translation="MYAFKTERPDAARRYHNKFVTGTNYQEESNRRFALSEPTENILR
                     TSILESGWLIKNITLRDVNAVSGNAVNIGASELYTGRVDGGRFHKKMAINGTEFFLTE
                     TDTCARISYSDMSHIYHTGVPDEFEKTVESFFAQAYALDMLRVGFNGVSIANTTNPEI
                     NKKGEDVNIGWHALAKAYGNGKQIISEPVTLGETGTWKNIDALANHLITELIAEQFRE
                     DPRLVVLVGAELAAHQRLKLFNAADRPSDVNAAQMATSSVAGRFAFIPPFMPGKRLAV
                     TTLDNLHIYTQAITRWFRAEFDDESSEYVHSYLRNEGYALGEPELYAAVDECTLTFAD
                     "
     gene            complement(7548..8408)
                     /locus_tag="QBH27_004133"
     CDS             complement(7548..8408)
                     /locus_tag="QBH27_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016381.1"
                     /translation="MVDNVFKKKLASIKNEHVSVLDSYKVRSFKETHSDTACIVRIIE
                     IYSLNKLRAKGEKLYSLTGLTVPDTETVANEINLLLSRYAQLCRQEEEELSFRQREVT
                     NAEVAWKSTFSKNGVSSIAEAKTNKMGHAERADAERYYHLAVSRLNEQHSRLSTIKLL
                     PGVLADEGNYIGKGIDKRLLNIFPQSGQIPADFISVFNDSDVVRDIKFITDALKSLSD
                     SVSEIISRCSVPTDRYVLNNGGMARAMAYREYYRADNYVLRSVVSDRDYVEHVMKYNL
                     VTEYKNKIFS"
     gene            complement(8426..8653)
                     /locus_tag="QBH27_004134"
     CDS             complement(8426..8653)
                     /locus_tag="QBH27_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ7016382.1"
                     /translation="MSLLPRENAPQKRYRLLRISEVINLTGLPKSTIYLKIKNDEFPN
                     QVSIGSRSVAWVEHEVNEWIEKNILNRKLNS"
BASE COUNT         2414 a         2234 c         1754 g         2528 t
ORIGIN      
        1 tatcagagtt ggataatgat ggattatcat atagaagtgc aactcgtcta tttaagttag
       61 cattcaacca attattaata tcattaatcg ttgtattggg ctgaataaga tatccagaga
      121 tgatattatt tttatttgtt aatacacttg ataaattttg atttaaagtt aagacgttat
      181 tgtgtgaaga aaaatcttcc tgataaggat taaggataac aattatgtca gaattccaaa
      241 catttaaaca tgttaataaa tttctcgtat tgacgttaat tggttctaaa attggtagca
      301 gaccatttag gctaactccc ttcgcaacga ggtccctaag gcataataac tctgtacttc
      361 tcgcatacaa atatgggcta tacataactt acaactcctg aggatatcaa caaattttaa
      421 tttttacatc catcaaattc atgatgaatt ctttttgctt tcttgataat ggtgttgcat
      481 aacctaaaac acgtaaacat gctggaagag aataaatata atccaaaaaa actttatcgt
      541 tacatctttt tttcatagca gagataaagt atttatttgc tatgtttaat ggcatctgca
      601 tgaatatctc tttacaatat tcataaacca atccgttagg catatttatt ttagttgaag
      661 tgtaatcttc tattatagct aagaactctt cctttctaag aactgaaaac aagctgtcac
      721 ttttaatata atcaatattt gaatctgcct ccttaatgac actcaattga tcttttttat
      781 tcaaaaccat aactcccact ctggggagat ttaaagcaca atatttactt gcatactctg
      841 gatgtgtaac tatatacact ttatcaaaaa catccaaata atcaggggct tgagtaatca
      901 acctttttgg tgaatcatac tctgtcttaa tctcgtaagc tgtagacgta ccattaaaca
      961 ccgcaacatc cactatagag tttttaattg gtaactctat ggacgacgaa cttgtttttg
     1021 ggctgtgctt accgaatatt attttattga caagtgctgt cttataaaca tattcatttc
     1081 tataattcag acgcaaaaaa tcatataatg atgtaaataa atcccttagt gaaatctcat
     1141 gttcattatt atcattgttt gaaaacagtg tttcatagca gtaagaatca aaactcccaa
     1201 ataaatccga aatatcctta ccttttgcta aggatgaaaa aatggggcga cggaaaaatt
     1261 tagcatattc ctttttatta tgactcatta ctcatactct ataaacatca tgcaatttga
     1321 taagttatga cgaattctca ccatccagtt actacctgta catactaatc aaaaaaaacg
     1381 agtacgcaag atacgaaagt acttttcttg ttcaccagtt agcttacctt gatcctcacc
     1441 agcgactgga agataggtag cagtatatgt ggaaaccttc ccctagctag gtctacaaac
     1501 gtagtttggg catttaggct ccacgattca cggtttgaca cggcctgata tctgcacatt
     1561 cgttttgata tgcagcaatc gtgaaaaagg aaaatatacg ctcgacagac caaaacggca
     1621 ctacaccgca cccgcctgcg gttttcatat cactaaaaat tttcagcctg gattttttac
     1681 aaacgatacc gccagtccgt gccagtcctc gagatgtact gcgatacagg aactgaaaag
     1741 aatgaaaaga aattcatttt ttttcagttt tactgtccgt cgaatctatg gcctgaatac
     1801 gcaactctat gatattaaat ggataattaa ttttaggtgt gtgattttat gaaggtttgt
     1861 gaaacatgaa gaagataacc agttgaaaaa taataatttt atgaccgtat aaactgaaat
     1921 cctgtgttca ggttccggga acagatcggc cattcccgac gcatacttta cccattcttt
     1981 tgcgcagcca gcagcaactt taaagccttt tcccgttcct cgggtggaat tgcatcaatc
     2041 agctctttaa cccttccctg cccgttcagc gcgcttggac taacggaatg ggagaaagca
     2101 acattcatca caaacgagtg accgcactcc acattagagc aatagcagta cacatcgtac
     2161 agttgtgggt gtttcctgtt tgttttacct atccgtgcta ctgcctgaca tgtggggcag
     2221 tacattctca tcatcttcat cgttctgtgc cctatactgt tgcccgccag aagaagggat
     2281 ttggatccct acctcttgtt ctgaacgtgt aacactggta acacgtgtaa cgctattgat
     2341 ttaaaaggat ttaatctgtt accagttagc aaaatcaaga ggtaacgcgt ggtaacacac
     2401 cattttttgt tacctctgtt accagtcagt atttttgact ggtaacactg aagaaccgca
     2461 tcagtaacgg gtgttacctc tgttaccagt gttacctcat aaaaataaga ctcacgcgat
     2521 aatcactcag gttcagcgct tcccagtacc tgtgtattga actggtacac acgtttaagc
     2581 cctatttctg gcaggcgaat gctgttttgc gtccggccat cttcaccggg tttcagccag
     2641 cccgcgtcca cacacaacct ggccacctta cgcgagtcaa atcccttaca gatctccttc
     2701 cagcctgacg ggagaacgta gaacgtcaca accggctctg tcacgttgtc tcctttatcg
     2761 acttttctga atcccatcat tgaaacaggc cggttcctgt cgtcattcca gtcagcaaac
     2821 cgactgaact gattacgcgt catgaagtcc cgaacctgtt caagtgccgc tttatcttcc
     2881 tgattagctg tatggccccg gtcagccatc catgcagcca gacagctttg agcggcacga
     2941 aaggcctctc cctccggcca tcctgtgatg cccgcttttg tggccagctc cccggccatc
     3001 gcaaccagag caaaacgggt aacagcccgg ccaacctgat tcccggcatt ttccggcgtc
     3061 agccttcggg tatactcttt caataaggct ttcgcctgac tggtcagttc cggcagatca
     3121 gcggtcaggc aatacagcca gtcgcggaat ggtgcaccat gataatgcgc tacgtgctgc
     3181 tcaagatgct cagccagggt tttcccgcta ctgaagccat gaagttcttc aaacacgcca
     3241 tacttgcccg aatcgcttgg gatctggatc attctgacct caaccccggc atacgtacgt
     3301 tccccggcgc ttgccgcatg ctctaccagt gacagttccc cggtagacag aaacaacaga
     3361 ttccagcggt tggtttccct taccgaacca tctgttctgg ctctggcttt gccctgcccg
     3421 ttagccagca tataagcgat atttcccgcc tccctgccgt caacctcccg gatttcatcc
     3481 agcatcagcg tggcatcatt gcggcggctc gccgttcctt ctaaggcatt ccccgtcgcc
     3541 cgccacgtat gccagaaatc tgttccacca catactgatg cagccacttt catcgtcgtg
     3601 gttttaccgt ctgtggattc ccctttgagg tgataacctc caccgccgat accaaccagt
     3661 ttcagaaggg gggcagcaaa tgccagactc accgcaaagg caagacgggc attctttatg
     3721 caataacggc ctatattttc acgccagccc tctgatgtgc cgctgacacg aaaatcacgc
     3781 ccctgcacac tcgatgtttg taagatgact gactgggctt cgcgccctat cacttcatcc
     3841 tggagaacgt aaaccccgcc gtgccagcca gttttattca cacaggttac ttttctgtcc
     3901 ggtttacaca gcgagatata ttccatcagg aatgctctgg ccatgccgtt aatgttgatg
     3961 taagaaagcc cgttaaccag cagaacgcgg cgcagttctt ccccgctacc gcccagcatc
     4021 tccatcggca ttgcccattt acggctgttt ccgttggtat cttcccattc cagcaaacgc
     4081 ccgtaattac ttccatcagc atcactggtg attgccgtca cccgcaacgg actgcaaatt
     4141 ttgatattac gtatctctgt atcaccatca gacttattaa ccagcttgtc gtaccacaaa
     4201 tattcttttg tcagccggaa cccttctggt aaacgggtct gccctttgcc atgcagcgtc
     4261 atttcttcac gaaaggcatc tctcgcccgt tctttcccca cttcctgccg ataatcatcc
     4321 cagtcagcct tatggcgtac cggagggagc gttacccagc catccaccgc tttggcggct
     4381 ttctctgcct gaatacgccc ggtgttttct ttgccgtcag ccagatcatt atctccggcc
     4441 agaatgatcc gggtttccgg ccagcgtttt cggatctgct ccgtcacctt cagtaaattg
     4501 gtggcagcga cagccgccac gatccatcct tcagtcagca gactgacggt aagcgccgtg
     4561 gcaaaaccct cagtgataat cacctgttca ggtgtttcag cgggtatatc ggtcagggca
     4621 atgaatgcgc cagacagctg gctaccgggc agcagacttt tatccccgtc aggattgata
     4681 agctggccac cagtaatatt cccggaaata tccgtcaaag gcagcagtaa tgaaccggtg
     4741 gaaaatgtta ttccggccag attcaaaggc tgtgacagca agggaagtga atacccgtgt
     4801 aagctcttat ttgtcagata gaccggctca ccattgcagg actgctgtct gagtctggta
     4861 tatcgctccg cgcccgcttc ttttcctgcg gcctttttcc tggctggcaa tgcgggcttc
     4921 tcctgtattt ctggtaatgc aagcgcctca gataccatcc cggcgacttc ttttatcttt
     4981 cttcctgtca ccagacgaac cagatcaaga ccatcgccat gaccgcactg attacaaaac
     5041 cacgtccctc gccccgcttt atcgtccagt cgaaaacgat cttttccacc acaggcaggg
     5101 caagccccat gatgtcctgc ggacggtact ttaataccta atgctgacag gatataaggc
     5161 cagcgcccaa ttgcagctcc ggaaatcgtg gatacagacg gtgttttcac agcagcacct
     5221 cctgaccttc cggcttcaca aattcaaaac cttcgggtag ttcgccatca ggtgctccga
     5281 aaataatatc ccggtaaacc tcacgcatct ctgtgacacc aaaaactgac aggcgaatat
     5341 tttctgacat aaagcctggt ttaagcattt tttccagttt ctctgcggcc agtatgcaac
     5401 cagcatcagc cccaaactca ttcacccacg gcatttcaat atggtaaata atattactca
     5461 ccactttttc gtgggagagt tctattcgtt cgccatcttc aatataaaca ggttcattat
     5521 gaacacgcac cattaaggaa atgaatgcat cagcaatgta gtagcgtaag actgctgttc
     5581 gtaaagcatg cgcctttaaa ttttgattat tattcatcgc gcatcactcc cttaattgtg
     5641 ctggtttcct taacaacgcc atccagttgt tcggcaatac aggccactaa agccgcagcg
     5701 ccttccgctg aaacagttct cgctccattc actgtgggca cggacaagac ctctgcaaga
     5761 aactcactgg ccagtgccgc ccgtaacaga cgactctccc cctgttcact caatatgcag
     5821 ccttgcttaa aattcatgga tttcatgcaa ccacctcccc ggctcttttc acagggatac
     5881 gggcagcaaa gctcaggata aattcagtgg caagtttaag gcgtgcagca tgctcgcatt
     5941 ctgcgattac acgcacaggg cgtggacggg catcacggtc tgtacggcgg acagccagaa
     6001 agacaaaggt aaattcaggg tgagataaag cagggactgt agccataatg gcaacctcct
     6061 tcagatagcg ggtaacgcta ccaccggagt tcctacgctc atgggtggta gcccagacgg
     6121 gggtaggaat accggctctg aaggataccg gccagcccga aagctgcccc gcctggacta
     6181 ccataattct gatgatgtgg cgtaaaaaat aaaaaaatac aatcagccac cacaccataa
     6241 attttaggtg agtcaaagct gcgacacaaa aaaacacgcc tagcgcgtgt agtatcgcct
     6301 tcagataaca cgggttccta cgcccggctg ccgattttgc ggcaaccttt aaactatatc
     6361 ccgcgcattc ctggtgaggc aagaaattta taaccgaaaa ctgatcattg cctggattaa
     6421 ttaacgctgg catgcttaag cctctgctta aaaatagttc ttcatttttc ctgatgctgt
     6481 aaacgagacc cgcctctgag cgggtctttc tgtcgctaag tcaatccgca aacgtcaggg
     6541 tgcactcatc aacagccgcg tacagttcag gttcaccgag tgcataacct tcattgcgca
     6601 ggtaggaatg cacgtattca ctgctttcat catcaaactc agcacgaaac cagcgtgtta
     6661 tcgcctgagt gtaaatatgc agattatcca gcgtcgttac tgccagacgc ttacccggca
     6721 taaacggggg aataaacgca aagcggccag caacagaact tgtagccatc tgtgccgcat
     6781 tcacatcaga cgggcggtct gcggcattaa ataacttcag gcgttgatgt gctgccagct
     6841 ctgccccaac cagtacaacc agacgaggat cttcacgaaa ctgctcagca ataagctctg
     6901 taatcagatg gttggccaga gcatcaatat ttttccaggt gccagtctct cccagagtga
     6961 caggctcaga gatgatttgc tttccgttcc cgtaggcttt cgccagtgca tgccagccaa
     7021 tattgacatc ctcgcctttt ttattgattt ccgggtttgt agtatttgca atcgacacac
     7081 cgttgaaacc gacccgcagc atgtccagcg catatgcctg ggcaaaaaag ctctcaaccg
     7141 ttttctcaaa ctcatccggc acgccggtat ggtaaatatg agacatatcg gaatagctga
     7201 tccgggcgca ggtatccgtc tctgtcagaa agaactccgt accattgatt gccatttttt
     7261 tgtggaaacg gccaccatcc acacgtccgg tatacagttc actggcacca atattgacgg
     7321 cattacctga cacggcgtta acatcacgta gggtaatatt tttaataagc cacccggatt
     7381 ccagaattga ggtacgcagt atattttcag taggttcgct taatgcaaag cgtctgtttg
     7441 attcttcctg atagttagtg ccagtgacga atttattatg ataacgacgt gctgcgtccg
     7501 gcctttcagt tttgaaggca tacatagttt ttcacctttt taagtattta agaaaagatt
     7561 ttatttttgt actcagtaac cagattgtat ttcattacat gctcaacata atcccggtca
     7621 cttacgactg aacgtaatac ataattatct gctcgatagt actccctgta agccatagct
     7681 cttgccattc caccgttatt taatacataa cgatcagttg gaacgctaca acgactaata
     7741 atttcactca cggaatcaga taaagacttg agtgcatcag taataaattt aatatcacgg
     7801 acaacgtcac tgtcattaaa tacagaaata aaatcagcag ggatctggcc ggattgagga
     7861 aatatattca gtaaacgctt atcgattccc ttcccaatgt agtttccttc atcggccagt
     7921 actcccggca aaagcttgat cgtgctcaat cgactatgtt gttcattcaa ccgggaaaca
     7981 gccagatggt aataccgttc agcatccgct cgttcagcat gccccatttt attcgttttg
     8041 gcttcagcaa tgctactgac gccgtttttt gagaatgtgc ttttccatgc aacttccgca
     8101 tttgtcactt cacgctgccg gaaagataac tcttcctctt cctgacgaca caactgggca
     8161 taacggctca ggagcaggtt gatctcattc gccacagttt ccgtatcagg tactgttaac
     8221 cccgtaagtg aatacagctt ttcaccttta gctcgcagct tattcagtga atatatttca
     8281 ataatacgca caatacaggc cgtgtcgcta tgagtctctt taaatgatct tactttgtaa
     8341 ctatctaaca ccgacacatg ttcgttctta attgatgcca atttcttctt aaaaacattg
     8401 tcaaccatat atttgccacc tcaatttagg agtttaattt tctattgaga atattcttct
     8461 ctatccattc attaacttca tgctccaccc atgcaacaga acgagaaccg atagaaactt
     8521 ggttcggaaa ttcatcattc tttatcttta gataaattgt tgacttgggt aagccagtca
     8581 aattaatcac ttctgatatt ctcagaagtc tatatctttt ttggggtgcg ttttcccgag
     8641 gcaataagct catggctatg tccttcacat tagatattta tactaagact taattagtct
     8701 ctattgggtg tatatatact tgcacagaag aattaatacg taaagactgg cactttcaaa
     8761 ctcattcaaa gaatttgaat actaaataat ggtgaataat agttaataat ggtgagtatt
     8821 actgagtgac aacctttgcc aatgcttgtt aatctctgat aataatgaaa tttgacgaat
     8881 aatatatgta acaatttgta aactaaagac tgcattaggt ttgttggggc
//
LOCUS       DAOFSV010000169         8843 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.169, whole genome shotgun sequence.
ACCESSION   DAOFSV010000169 DAOFSV010000000
VERSION     DAOFSV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.169"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            58..567
                     /gene="yihI"
                     /locus_tag="QBH27_004135"
     CDS             58..567
                     /gene="yihI"
                     /locus_tag="QBH27_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDQ7016383.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            756..2129
                     /gene="hemN"
                     /locus_tag="QBH27_004136"
     CDS             756..2129
                     /gene="hemN"
                     /locus_tag="QBH27_004136"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDQ7016384.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(2362..2472)
                     /locus_tag="QBH27_004137"
     CDS             complement(2362..2472)
                     /locus_tag="QBH27_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDQ7016385.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(2584..3993)
                     /gene="glnG"
                     /locus_tag="QBH27_004138"
     CDS             complement(2584..3993)
                     /gene="glnG"
                     /locus_tag="QBH27_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDQ7016386.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(4005..5054)
                     /gene="glnL"
                     /locus_tag="QBH27_004139"
     CDS             complement(4005..5054)
                     /gene="glnL"
                     /locus_tag="QBH27_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDQ7016387.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(5228..6637)
                     /gene="glnA"
                     /locus_tag="QBH27_004140"
     CDS             complement(5228..6637)
                     /gene="glnA"
                     /locus_tag="QBH27_004140"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDQ7016388.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            7010..8833
                     /gene="typA"
                     /locus_tag="QBH27_004141"
     CDS             7010..8833
                     /gene="typA"
                     /locus_tag="QBH27_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDQ7016389.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         2120 a         2349 c         2343 g         2031 t
ORIGIN      
        1 tctgttagat tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg
       61 aaaccatcat cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc
      121 gaggagctgg atcaggaagc tcgtgaccgt aagcgtcaga aaaaacgtcg tggtcatgcg
      181 ccgggcagcc gcgcagcggg cggtaacacc acgtcaggca gcaaaggtca gaacgcacca
      241 aaagatccac gtattggcag taaaacccct attccattgg gcgtgactga aaaagtcacc
      301 aaacagcaca aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggagtta
      361 ctggaaacgg atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg
      421 agcgccgaag agcaatcctg ggtggatgcc aaactggatc gtattgatga gttgatgcag
      481 aaactcggcc tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac
      541 atgatgcgtc tgctgcgggg caactaacgg attgccgccg tgggccttcc cgttctgctg
      601 ataacccttc cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg
      661 cggcgacaaa agtggctgcg caaccggctg atgactcgaa acgggcatcc gccggtacgc
      721 cgtagccgcc agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg
      781 atctggccct gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg
      841 cgctggagtt ttcagaagac ttcggcgaac aggcgttttt acaagctgtg gcgcgctatc
      901 ctgagcgtcc attatctctc tatgtacata ttccgttctg ccataagctt tgttacttct
      961 gcggttgcaa taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc
     1021 tggagcaaga aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc
     1081 actggggcgg cggaacgccg acgtatctga ataaagcgca aatcagccgt ctgatgaagc
     1141 tgctgcgcga aaacttccag ttcaatgccg atgcggagat ctcgatcgaa gtcgatccgc
     1201 gggaaatcga actggatgta ctcgatcatt tacgcgccga aggctttaat cggctgagca
     1261 tgggcgtgca ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag
     1321 agttcatctt tgcactgctt aaccatgcgc gtgagattgg ctttacctcc accaacattg
     1381 acctgattta cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaagcgtg
     1441 tggcggaact gaaccctgac cgtctgagcg tctttaacta cgcgcatttg ccgaccattt
     1501 ttgccgccca gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata
     1561 tcctgcagga aaccatcgcc ttcctgacac aatcgggcta tcagtttatc ggtatggatc
     1621 actttgcccg tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta
     1681 acttccaggg ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca
     1741 tcagcatgat tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc
     1801 aagtggatga acaaggcaat gcgctgtggc gtggtattgc gctaacgcgt gatgactgta
     1861 ttcgccgcga tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg
     1921 agcaacagtg ggatttgctc ttcgctgatt actttgcgga agatctcaag ctgctcgccc
     1981 cgttagcaaa agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag
     2041 gtcgcttgct gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc
     2101 ggatgcagca gttctctcgg gtgatttaaa ttgtgaatgg cgcttcgttt acaaagtaga
     2161 aaacgaaagc gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg
     2221 cctgcaaaat ctgttgaaat tattgctttt gtaggccgga taaggcgttt acgccgcatc
     2281 cggcaatcaa cgcctgatgc gacgctggtt gcgtcttatc aggcctacga ctttctccgc
     2341 atccggcgta aacaacgagc ttcagctaaa cagccctatc atcgcggcac acagcacggc
     2401 agcaacagca gtttgcggtg tgtggctgtc aactgcgcca ctcgatacca gattaattat
     2461 tgattccagc ataatgactc tccccgtttt ccgggcaaga tcatactgaa cttatcggaa
     2521 cagtaaagcg caaaatacca gcaatttgcg ctcaataatc aatctttaca cacaagccgt
     2581 gaatcactcc atccccagct cttttaactt acgcgtcagg gtgttgcgac cccagccaag
     2641 tagccgcgcc gcttcctgtt tatgcccctg cgtatgtcgc agcgcggtgg tcagtaacgt
     2701 ccgttccagc tctggctgtg cttcggaaag caggttttga tgaccggaac gcagcgctct
     2761 gtctgcccac tgtgctaaca gcgttgccca actgtccggc tgcatgtgtg aagtgctttc
     2821 cggtacgttt gattcaaaca gttcgccagg caaatcctga atcaacactt cctgcccggc
     2881 agccatcacc gttagccagc gacaggtgtt ttccagctgg cgcacgttgc ctggccacgc
     2941 cagacgcgtc agcgcggctt cggtttccgg atgcagcaac ttcgcttcta cgcccagctc
     3001 tcgcgcggca acctgtaaaa aatggcgcgc cagacgggga atatcttccc gacgttcgcg
     3061 cagcggcggc agatgaacgc ggatgacgtt caggcggtgg aacagatcct cacggaactt
     3121 gccttcctgc acccgctgtt caagattctg gtgagtggca gcgataatcc gcacatctac
     3181 tttcaccggc gcatagccgc caacgcgata aaactgaccg tctgccagca cgcgcagcaa
     3241 acgcgtctgc acatctagcg gcatatcgcc aatttcatcg agaaataacg tgccgccatc
     3301 agcctgttca aaacgcccct gacgaatggt attcgcgccg gtaaatgcgc ctttctcgtg
     3361 accaaacagt tcggattcga tcaaatcctt cgggatagcc gccatattga gcgcgataaa
     3421 cggcgctttg gctcgcggac tgtggcgatg cagggcatga gcgaccagtt ctttaccggt
     3481 gccggattcg ccgttaatca gcacgctaat agaagaacgc gaaagccgac cgataatccg
     3541 gaacacgtcc tgcatggctg gcgcttcacc gatgatatcg gtcgttgggc cgttaagctg
     3601 aacattacgc ggctgctgct gttcctggta atgactgatg gcgcgctcaa ccagcgccac
     3661 ggcttcgtcg atatcaaacg gtttgggcag ataatcaaat gccccttgtt gataggcgct
     3721 gacggcagca tccagatcgg aatgtgcggt cataatgatg accggaagca tcggatggcg
     3781 ctgtttaatc tgcttgagca gcgccagccc gtccattccc ggcatacgga tatctgaaag
     3841 cagcacatcc ggcgttttgc tcgccagcgc ctccagtact tccgcgccgt tctcaaatgt
     3901 cgtacaggtt aaacccgctc cagcgagcgc acgttcaagc acccaacgga tggaactatc
     3961 gtcatcgact acccagacta tccctcgttg cataaacgtc acctttattt cctgataggc
     4021 aggtaaaccg agaactcggt atgacctggc caactggtaa attcaatttt gcctgaatgc
     4081 tgatcaatca aattacgggc gatggataag ccaagcccgg tgccaccttc gcggccgctg
     4141 accatcgggt aaaacagcgt atcctgcaaa tgaggcggaa tgcccggccc gttatcttcc
     4201 acatcaatcc gcgccgccag ccgatagcgc tcgccgtgta aggtcagttg aaacgctgtg
     4261 cgggtacgta gaatgatttc accaccctcc ggccccagcg cctgtagcgc attgcgcaca
     4321 atattcagca agacctgttc aatttgatcc gggtcgtgcg ccagttccgg caggctgggg
     4381 tcgtaatccc gaatcaaccg cacgttgttc ggcagttcca tcgacaccag cgtcaccacg
     4441 cgttcagcca ctttgtgaat actttcggta atgcgcgtac cgggcagctg cggccccaac
     4501 agacggtcga ccagatttcg cagccggtcc gcctgttcga taatcacttt ggtatattcg
     4561 agtagtgacg gatcaggtaa tgctttgctg agcagctgcg ccgcgccacg taaaccgcca
     4621 agcggatttt taatctcatg tgccaggccg cgcactaaat cacgggcagc aacctgctgg
     4681 gcgtgctgta gctgttcctg acttaagcgg cgctggttat ccatcggtgc catctccagc
     4741 aggatcatgc cgtccggcat acgctgagcc gtcacagaaa ggatatgcga gcgcccgtcg
     4801 atgaccagcg tcacttcgtt atcggtaaaa ccttgccccg cctccagact ttcttgcatc
     4861 agctcgatat ttaatgagaa gtagctcaac agttccggta acggtgtacc aaacaatttg
     4921 cgggagcttt gggcgagcag ttgttgcgcg gcggggttgg cgtaatggat cgccaggttg
     4981 tcatcgatta acaaaatact gttaatcagc gagttaagga tctgcccagc atcgggctgc
     5041 gtgcctgttg ccataaagca gtctcctgaa caggttgcac cattttagtg cattatagct
     5101 ttttacggat aaaaagcgcg aagcatcaga aaattgacgg agaaaaaagc ccatgcagag
     5161 atgggctgaa agtttccacg gcaactaaat cccggcgttg ttgcgccggg tagtacaact
     5221 tcaactatta gacgctgtag tacagctcaa actctaccgg atgcggagtc atacgcacgc
     5281 ggtcatcttc ttcgcgacgc agagcgatgt acgcatcgat cgcttcatcc gtgaacacgc
     5341 caccggcttt caggaactcg cgatccagat ccagttcgtt cagtgcttct tccagagagc
     5401 ctgcaacctg tgggatctct ttcgcttctt ctggcggcag gtcatacagg tttttgtcca
     5461 tggcttcgcc cggatggatc ttgttcttga tgccatcaag accggccatc agcagggcag
     5521 caaagcacag gtacgggtta gccgccgggt ccgggaaacg tacttcgata cgacgtgctt
     5581 tcggagaaga aaccaccgga atacggatag acgcagaacg gttacgcgca gagtaagcca
     5641 gcattaccgg tgcttcgtag cccgggacca gacgcttgta agagttggtg gtcgggtttg
     5701 ccagggcgtt aatcgcttta gcgtgtttga ttacgccgcc aatgtagtac agcgcctgtt
     5761 cagacagacc tgcgtatttg tcgccagcaa acaggttgac gccgttttta gacagcgaca
     5821 tgtggcagtg cataccggaa ccgttatcac cgaacatcgg ttttggcata aaggtcgcgg
     5881 tcttaccgaa acggtgcgcc acgttgtgca cgacatattt gtagatctga atttcgtcag
     5941 cttttttggt catggtattg aagcgggtag ccacttcgtt ctgacctgcg gtagcgactt
     6001 cgtggtgatg agcttcaacc accagaccca tctgttccat caccaggcac atttcagaac
     6061 ggatatcctg agctgagtct accggcggaa ccgggaagta accgcctttc actgccggac
     6121 ggtgaccttt gttaccacct tcatattggg tggaggagtt ccatgcacct tcgatatcat
     6181 cgatagcaac gtgggaaccg gagatagacg atccgaaacg gatgtcatcg aacaggaaga
     6241 attcaggttc tggcccgaac agtacggtgt cggcaatgcc agtagaacgc aggtaatctt
     6301 cagcgcgctt cgcaatagag cgcgggtcac ggtcatagcc ttgcagggtg ccaggttcaa
     6361 ggatgtcgca acggataatc agggtagaat cggcgaagaa cgggtcaatc actgcggtgg
     6421 atgcgtctgg catcagcacc atgtcggatt cgttaatgcc tttccagccg ccaatcgagg
     6481 agccgtcaaa cattttgcct tcttcgaaga attcggcatt cacctgatga gcagggatag
     6541 tgacgtgctg ttctttacct ttggtatcgg tgaagcgcaa gtcaacaaac ttcacttcgt
     6601 gctcgttcag catcgtcagt acgtgttcag cggacatact ttaactctcc tggattggtc
     6661 attgtcgtcg tggtaacgaa atctgcaatt aaatgggccg tgtcgccgta aaaaagataa
     6721 agcgaaaact gtgccaactt ttaaattgcc cctaaaaggc gttatcatgc ttaacatcgt
     6781 gcaaaagggc tgcaccatga tgtgaatgtt gcaccaatat agtgcttcaa tgggaacatt
     6841 aagcaccata ttggtgcaat gacctttgga taaccctttt tatgctccgt gaaagcgatc
     6901 acaaagggac tctgcaatac ttgtttgcgg aggatgtttg tgatcctgtt ttgtagtgcg
     6961 attaatccgt gtacaataac gcgctatttc taatgcctga ggcaaagttg tgatcgaaaa
     7021 attgcgtaat atcgccatca tcgcgcacgt agaccatggt aaaaccaccc tggtagacaa
     7081 gctgctccaa caatccggta cgttcgactc tcgtgccgaa acccaagagc gcgtgatgga
     7141 ctccaacgat ttggagaaag agcgtgggat taccatcctc gcgaaaaaca ccgctatcaa
     7201 atggaatgat taccgtatca acatcgttga taccccgggg cacgccgact tcggtggtga
     7261 agttgaacgt gtaatgtcca tggtagactc agtgctgctg gtggttgacg catttgacgg
     7321 cccgatgccg caaacgcgct tcgtaaccaa aaaagcgttt gcttacggcc tgaagccgat
     7381 tgttgttatc aacaaagttg accgccctgg cgcgcgtcct gattgggttg tggatcaggt
     7441 attcgatctg ttcgttaacc tcgacgcgac cgacgagcag ctggacttcc cgatcgttta
     7501 cgcttctgcg ctgaacggta tcgcgggtct ggaccacgaa gatatggcgg aagacatgac
     7561 cccgctgtac caggcgattg ttgaccacgt tcctgcgccg gacgttgacc ttgacggtcc
     7621 gttccagatg cagatttctc agctcgatta caacagctat gttggcgtta tcggcattgg
     7681 ccgcatcaag cgcggtaaag tgaagccgaa ccaacaggtt actatcatcg atagcgaagg
     7741 caaaacccgc aacgcgaaag tcggtaaagt gctgggccac ctcggtctgg aacgtatcga
     7801 aaccgatctg gcggaagctg gcgatatcgt ggcgatcacg ggcctgggcg aactgaacat
     7861 ttctgacacc gtttgcgaca cgcaaaacgt tgaagcgctg ccggcactct ccgttgatga
     7921 gccgaccgtt tctatgttct tctgcgttaa cacctcgccg ttctgcggta aagaaggtaa
     7981 gttcgtaacg tctcgtcaga tcctggatcg tctgaacaaa gaactggtac acaacgttgc
     8041 gctgcgcgta gaagaaaccg aagacgccga tgcgttccgc gtttctggtc gtggcgaact
     8101 gcacctgtct gttctgatcg aaaacatgcg tcgtgaaggt ttcgaactgg cggtatcccg
     8161 tccgaaagtt atcttccgtg aaatcgacgg tcgtaaacaa gagccgtatg aaaacgtgac
     8221 tctggacgtt gaagaacagc atcagggttc tgtaatgcag gcgctgggcg aacgtaaagg
     8281 cgacctgaaa aacatgaatc cagacggtaa aggccgtgta cgtctcgact acgtgatccc
     8341 aagccgcggt ctgattggct tccgttctga gttcatgacc atgacttccg gtactggtct
     8401 gctgtactcc accttcagcc actacgacga cgtacgtccg ggtgaagtgg gccagcgtca
     8461 gaacggcgta ctgatctcta acggtcaggg taaagcggtc gcgttcgcac tgttcggtct
     8521 gcaggaccgc ggtaagctgt tcctcggtca cggtgcagaa gtttacgaag gtcagattat
     8581 cggtattcac agccgctcta acgacctgac tgtaaactgc ctgaccggta agaaactgac
     8641 caacatgcgt gcttccggta ctgacgaagc cgttgttctg gttccgccta tccgcatgac
     8701 tctggaacaa gctctggagt tcatcgatga tgacgaactg gtagaagtga ctccgacctc
     8761 tatccgtatt cgtaaacgtc acctgacgga aaacgatcgt cgtcgcgcta accgcgcacc
     8821 gaaagacgat taatttcgtt ttt
//
LOCUS       DAOFSV010000170         8606 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.170, whole genome shotgun sequence.
ACCESSION   DAOFSV010000170 DAOFSV010000000
VERSION     DAOFSV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.170"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(37..495)
                     /locus_tag="QBH27_004142"
     CDS             complement(37..495)
                     /locus_tag="QBH27_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDQ7016390.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(492..1409)
                     /locus_tag="QBH27_004143"
     CDS             complement(492..1409)
                     /locus_tag="QBH27_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDQ7016391.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            1555..2232
                     /gene="fetA"
                     /locus_tag="QBH27_004144"
     CDS             1555..2232
                     /gene="fetA"
                     /locus_tag="QBH27_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDQ7016392.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2219..2998
                     /gene="fetB"
                     /locus_tag="QBH27_004145"
     CDS             2219..2998
                     /gene="fetB"
                     /locus_tag="QBH27_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDQ7016393.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3181..4035)
                     /gene="cnoX"
                     /locus_tag="QBH27_004146"
     CDS             complement(3181..4035)
                     /gene="cnoX"
                     /locus_tag="QBH27_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDQ7016394.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4096..4905)
                     /gene="ybbO"
                     /locus_tag="QBH27_004147"
     CDS             complement(4096..4905)
                     /gene="ybbO"
                     /locus_tag="QBH27_004147"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDQ7016395.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHREGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTRAVMVLKRLLPGRVMDKILQG"
     gene            complement(4895..5518)
                     /gene="tesA"
                     /locus_tag="QBH27_004148"
     CDS             complement(4895..5518)
                     /gene="tesA"
                     /locus_tag="QBH27_004148"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDQ7016396.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5489..6175
                     /locus_tag="QBH27_004149"
     CDS             5489..6175
                     /locus_tag="QBH27_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7016397.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6172..8586
                     /locus_tag="QBH27_004150"
     CDS             6172..8586
                     /locus_tag="QBH27_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7016398.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WATLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKAGEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFVG"
BASE COUNT         1924 a         2246 c         2389 g         2047 t
ORIGIN      
        1 ggttttggta cagatttcca ggtgtgataa gacgcgtcag gcgatgaccg cacggataat
       61 cagcgttatt ccttctatcg caatcacctc aacatgcgta ccagcgccga gatcctcgct
      121 ggcgctgaca ggccatgaac tgtcaccgac gcgcatatga ccgcgcccgt tgaccagcgg
      181 agattccaga acaaaacgtc ggccaatcaa ctgctgcccg cgctggttta aatgactgtc
      241 gctgtgcttt tgttcgcgca cccgccgcga caaccatttc caccacagcc aggcggcgag
      301 cagcgtcagg acggcaaaca ttaccccttg ccactcccaa cccagcggca gcaaccagac
      361 caccagaccg gtaatcaccg ctgccacgcc gctccacaac agataaccat tgccgcccaa
      421 catctcggct gccagcagca aaccgccgag acttagccag aaaatatgtg gatgaacgac
      481 cattaactcc atcatggctg agtccgtttg ttggcgctgt ctttcaccag ctcagcaatc
      541 ccggcaatcg accccatcag gctgctggcc tctaatggca tcatcaccac tttgctgtta
      601 ctggacgaac cgatctgctg taacgcttcg gtgtatttct gtgcgacgaa gtagttcacc
      661 gcctgaatat caccggaggc gatggcttca gacaccattt tggtggcgcg ggcttctgct
      721 tcagcggaac gttcacgcgc ttcagcctgt aaaaacgccg actgacgttc gccttccgct
      781 ttcaggattt gtgactgttt ttcaccttcg gctttgagga tttccgcctg acggatccct
      841 tccgcttcaa ggatgtaagc gcgtttggta cgttccgctt tcatctgcgc gttcattgaa
      901 gagataagct ctgcgggcgg gcgcacatca cgaatttcga tacgggtgac tttaatcccc
      961 cacgggttgg tggcctcatc gacaatatgc agcagacgcg agttaatgct gtcgcgctga
     1021 gagagcattt catcaagttc cattgaaccc agcacggtac ggatgttagt catggtcagg
     1081 ttgatgatcg ccagctccag attgctgact tcataagccg cgcgcggcgc gtcaatcacc
     1141 tgaataaagc acacagcgtc gatggtaacg ttggcgttat ctttcgagat aacttcctgg
     1201 gaagggatgt cgagcacttg ctccatcata ttgatcttgc gaccaatgcg atccataaac
     1261 ggcaccacca ggcttagacc tggctgtaac gttttggtat agcgaccaaa gcgttctacc
     1321 gtccattgat agccctgcgg cacgattttg acgcccgcgc cgacaatgac cagcgcgaca
     1381 aaaatgagaa tcgggataaa gataagcatc ggaaaaacct cctgttgtac cgtccataat
     1441 cagcaaaatt gctgcttgat taaacaaatt atacctgatt actgaaagag agttccccct
     1501 tattcctgcg aaggataaac tgtttttagt aaaaatcaga aaaagggaac agcgatgcag
     1561 gaaaatagtc ctttgcttca gctacaaaac gtaggatatc tggcgggtga tacgaagatt
     1621 cttaataaca tcaatttttc gctgcgtgct ggcgaattta agttaattac cggtccttct
     1681 ggttgtggca aaagtacact gctaaaaata gttgcttcat tgatcagccc aaccagcgga
     1741 acgttactgt ttgaaggtga ggatgtcagc acactaaagc cagaaatcta ccgtcagcaa
     1801 gtctcttact gcgcccagac gccgacgctg tttggcgata cggtatacga taacctgatt
     1861 tttccctggc agatccgtaa ccagcagcct gacccggcca tttttctcga ttttcttgaa
     1921 cgcttcgctt taccggacag cattttgacg aagaatatcg ccgagctatc tggtggtgaa
     1981 aaacaacgca tctcattgat tcgtaacctg caatttatgc cgaaggtttt attgctcgac
     2041 gaaatcacca gtgcgctgga tgaaagtaat aaacataacg ttaatgagat gatccaccgt
     2101 tatgtacgcg agcaaaatat tgctgtgctg tgggtgacac acgataaaga cgaaattaat
     2161 catgcggata aagtgattac actgcaaccg catgctggag aaatgcagga agcacgctat
     2221 gaactcgcat aatattacta acgaatcatt agcactggca ttaatgctgg tggtggtggc
     2281 aatcttaatt agccataaag aaaaactggc gctggagaaa gatattctct ggagcgtcgg
     2341 gcgcgcgata atccagctga ttattgtcgg ctatgtgctg aagtatattt tcagcgtgga
     2401 tgatgccagc ctgacattat tgatggtgtt atttatctgc tttaatgcgg cgtggaacgc
     2461 gcaaaaacgc agtaaatata ttgctaaagc ttttatctca tcgtttattg ccattacggt
     2521 cggggcggga attaccctgg cggtactgat cctctctggg tcgattgaat ttatcccgat
     2581 gcaggtgatc cctatcgccg ggatgatagc cggtaacgcc atggtagcgg tggggttgtg
     2641 ttacaacaat ttagggcaac gggtcattag cgaacagcaa cagattcagg agaaactgag
     2701 tcttggcgcg acgccgaaac aggcttcagc gatattgatt cgcgacagta ttcgcgcggc
     2761 tttaattccg acggtcgatt cagcaaaaac ggtaggctta gtgagtttac caggaatgat
     2821 gtccgggctg atatttgccg ggattgatcc ggtgaaggcg attaaatatc agattatggt
     2881 gacctttatg ctgctctcaa ccgccagctt gtcgaccatt attgcctgct atttaaccta
     2941 tcgtaagttt tacaattcgc gccaccaatt ggtggtgacg caattgaaga agaaatgatg
     3001 cgatgccgga tgcgtcttat cagacctaca tggcacctgc aatgtattga attagcacgg
     3061 tttggtaggc cggataaggc gttcacgccg catccggcgg ctatgcgcgg atgcctgatg
     3121 cgacgctggc gcgtcttatc aggcctacaa accagcacag catcgcattt caacggaaaa
     3181 tcaatacaac aacgcataca gctggcggcg atacttcgac gccagtgcat cacccgtacc
     3241 cagcgcagcg aggatctcct ggaatgtttt acgtgtctga ccatctgcgg cggtgagatc
     3301 tttacgcaga tgcccaaaca gtaactccag cgcctcttca ttgcgcccaa cctggtgcag
     3361 ttgcagtgcc agttgcgtcg ccagtgcggc atcttctgga ttctccgcca cctgctgttg
     3421 caactgttga atttccggcg tatcagccgc ctgcttcagc agttcgattt gcgccaccag
     3481 cccctgatag cgggtgtcct gatcctgcaa cggaatggtt ttcagcacgg cttctgcatc
     3541 ttcagaacgg ttcagcgcaa tcagcgtttc tgccaacagc aggccgattt ccccgttctg
     3601 attcgacaac tgccaggcgt ctttcagcaa tggcagggcg tcggtgtaat tgccttcctg
     3661 catcagttgc atcgcctgct gcgctttcag ctcttcttca cgcggcagca ctttatccag
     3721 cagggcgcgg atcgcctctt ccggttgcgg cccctggaag ccgtctactg gttgcccgtt
     3781 ctggaacaga tatacggtcg gaatcgcgcg cagaccaaac tgggcggcaa tcatctgctc
     3841 cgcgtcgcag tccagcttcg ccagaataaa ttgcccgttg tactgcgccg cgaggctttc
     3901 cagaattggg gttaactgca aacagtgctg gctacgttca gaccaaaaat agaacagcac
     3961 cggagtggtc atcgactgtt caagaacctg ttgcaggtta gattcgttaa tgttgacaat
     4021 attttctacg gacatggagt cgctctctgt tgtcgatatt ttctttgaca tgggggctta
     4081 agcgcgcgct tcaactcacc cctgcaatat tttgtccatc acgcgccccg gcagcaggcg
     4141 cttaagcacc attaccgccc tggtcaccag cgtcaccgga tagcgcatct tcggcttctc
     4201 gctaataaaa gcatggcgca ctttgccgac caccgcttcc ggtcccaacg taaagcgggc
     4261 ggcgatgcca ggattttcga ctggtttatc actttgcgtc tggttgacgt tgtcggtgaa
     4321 gcgagtacga atgggaccag gttcgatcag gctgacttta attccgctgt ggcgcagctc
     4381 catgcgcagt gcgtctgacc acgcctccag cgcatattta ctggccgcgt aagcgccacg
     4441 acccggtgtg gagattaatc ccatcaccga tgaggtcatc acaatacgcc cttcacggtg
     4501 cggcaacatc gcgggtaaca ggcgcatggt gagctggtgt gcgccgaaaa agttagcgga
     4561 aaactgctgt tccatctgcg cacggctgat ggtggaaagg gggccataca tgccgaatcc
     4621 ggcattgtta aagatcccat acagacaatt atcggtcagg gcgatcactt cgtcggctgc
     4681 gcgatcaaca ctttctggcg aatccagatc gatcaacacg ccggtaaatc ccatgctgtt
     4741 catgcgctca acatcatccg gtttccggca acctgccagc acatgaaaac cctggcgttt
     4801 taattcgagt gcgctttcca ggccaatacc actggaacat ccggtaatta agaccgattt
     4861 ttgcataact ttacctgtca ggatctccgt tgctttatga gtcatgattt actaaaggct
     4921 gcaactgctt cgccatccag tcggcaataa acggctgggc gtcgcggttg ggatgaatac
     4981 cgtcatcctg catccattgt ggcttgaggt agacctcttc cataaaaaag ggcagcagcg
     5041 gaacatcaaa ctctttggcg agtttggggt aaatggcgct aaaggcttca ttataacggc
     5101 gaccatagtt tgcaggcaga cgtatttgca ttaacaatgg ttcagcgttg gcggctttga
     5161 catcctgcaa aatctggcgc agcgtttgct cggtttgctg tggctgaaaa ccacgcaaac
     5221 cgtcattgcc gcccagttca accagcaccc agcgcggctg atgctgtttc agcagagccg
     5281 gaaggcgcgc cagcccttgt tgcgaggtgt caccgctgat gctggcattg actaccgacg
     5341 ttttactctg ccacttatca ttcaacaagg caggccaggc cgcgctggca gacattcgat
     5401 acccggcgct caggctatca cccagaatca ataacgtgtc cgctgcggcg gcacggaagg
     5461 ttaacaggac caggaacagg aagggcaaat gccagcggaa aacattgttg aagttcatca
     5521 tcttaagaag tccgtcggtc agggggagca tgaactctcc atcctcaccg gagttgagct
     5581 ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag tcgggatcgg gtaagtcaac
     5641 cttgctggcg atcctcgccg ggctggatga cggcagcagt ggcgaagtga gtctggtggg
     5701 acaaccgcta cataatatgg acgaagaagc gcgggcaaag ttgcgcgcga agcacgtagg
     5761 ctttgttttt cagtcattta tgttaattcc tacccttaac gcgctggaaa acgtcgagct
     5821 tccggcgctg ctgcgcggtg agagtagcgc agaaagtcgt aacggggcga aagcgttgct
     5881 cgaacagtta gggctgggta aacgtcttga tcatcttccg gcacagcttt ctggcggcga
     5941 acagcaacga gtggcgctgg cgcgagcctt taatggtcga cctgatgtgc tgtttgccga
     6001 cgaacccacc ggtaaccttg accgccagac gggcgataaa attgccgatc tgcttttttc
     6061 cctcaaccgt gagcatggca ccacgttgat tatggtgacc cacgacctgc aactggcggc
     6121 acgctgtgac cgctgcttac ggctggtgaa cgggcagttg caggaggaag catgattgca
     6181 cgttggttct ggcgcgaatg gcgctcgccg tcgctattaa ttgtctggct ggcgcttagc
     6241 ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg atcgcatgga gaagggctta
     6301 agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt tgcgcagttc gcgcgaagtg
     6361 ccgcaagcat ggctggagga agcgcaaaag cgcggcctga aagttggcaa gcagctgact
     6421 ttcgccacca tgacctttgc aggcgacacg ccgcaactgg cgaacgtcaa agcggtggat
     6481 gatatctacc cgatgtatgg cgatctgcaa actaatcccc ctggcctgaa accgcaggcg
     6541 ggcagcgtat tgctggcccc acgcctgatg gcactgctta acctgaaaac gggcgatacc
     6601 attgacgtgg gcgatgccac cttgcggatt gccggagaag tgattcagga accggattcc
     6661 ggttttaatc ctttccagat agccccacgc ctgatgatga atctggcgga tgttgataaa
     6721 accggagccg tgcagccggg gagtcgggtc acctggcgtt ataaattcgg cggcagcgag
     6781 aaccagctcg acggctatga gaaatggttg ttaccccagc ttaaacccga acaacgctgg
     6841 tacggtctgg aacaggacga aggcgcgctg gggcgatcga tagaacgttc gcaacagttc
     6901 ctgctgcttt cggcgcttct gaccttgctg ctggcggtgg cggcggtcgc ggtggcgatg
     6961 aatcattact gtcgcagtcg ctacgatctg gtggcgatcc tcaagacgct gggggcaggg
     7021 cgagcgcaac tgcgtaagtt aatcgtcggt cagtggttga tggtgctggt actttcagcc
     7081 gttaccggtg gggcgatagg cctgttgttc gaaaacgtgt tgatggtgct gctcaagccc
     7141 gttctgcctg ctgcactacc gccagccagc ctctggccgt ggctgtgggc gctcggcacc
     7201 atgacggtta tttcgctgct ggtggggcta cgaccatacc gcctgttgtt ggcaacgcag
     7261 cctttacgcg tattacgtaa tgatgtggta gcgaacgtct ggccgctgaa gttttatctg
     7321 ccgattgtca ccgtggtggt tgtgctactg ctcgccgggc tgatgggcgg cagtatgctg
     7381 ctctgggcgg tgctggcggg cgcggtagta ctggctttgc tgtgcggtgt cctgggctgg
     7441 atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc tacctctgcg cctggcggtt
     7501 agccgcctgt tacgtcagcc gtgggcgacg ttgagccagc tttcggcatt ttcgctctcc
     7561 tttatgctgc tggcactgtt gctggtgttg cgtggcgatc tgctcgaccg ctggcaacag
     7621 cagctaccgc cagaaagtcc taactacttt ttaatcaaca tcgctacgga acaggttacg
     7681 ccgctgaaag cgtttctcgc ggaacatcag atagtcccgg aatcgtttta tccggtggtg
     7741 cgggcgcggt tgacggcgat taacgacaag ccgacagaag gcaatggaga tgaggcgctc
     7801 aaccgcgagc tgaatctcac ctggcaaaat acgcggcctg atcataaccc gatcgtcgcc
     7861 ggtaactggc cgccaaaagc cggtgaagtg tcgatggaag aggggctggc gaaacgctta
     7921 aacgttgccc tcggcgatac cgtgactttt atgggcgata cccaggagtt ccgcgctaaa
     7981 gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc ctaatttcta ttttattttc
     8041 ccggaagggg cattagacgg gcaaccgcag agctggctta ccagtttccg ctgggagaat
     8101 ggcaacggca tgttgacgca actcaaccgc cagttcccga ccattagcct gttagacatt
     8161 ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg taagtcgggc gctggaagtg
     8221 atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc tggcacaggt gcaggtggga
     8281 atgcgtcagc gtcatcagga gctggtggtg tggcgcactc tcggtgcggg caaaaaactg
     8341 ctgcgcacga cattgtggtg tgagttcgcc atgctcgggt ttgtatccgg cctggtggcc
     8401 gcaattggtg cggaaacggc actggcggta ctgcaatcga aagtgtttga tttcccgtgg
     8461 gagccagact ggcgattgtg gattgtgctg ccgtgcagcg gtgcgctact gctgtcgctt
     8521 tgcggcggct ggctgggtgc gcgactgctt aagggtaagg cgctattcag gcagtttgtg
     8581 gggtgatgtg ataaagccgt ttattt
//
LOCUS       DAOFSV010000171         8598 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.171, whole genome shotgun sequence.
ACCESSION   DAOFSV010000171 DAOFSV010000000
VERSION     DAOFSV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.171"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1390
                     /locus_tag="QBH27_004151"
     CDS             <1..1390
                     /locus_tag="QBH27_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ7016399.1"
                     /translation="ENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDA
                     WLDSRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAAL
                     QSYTVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAE
                     AIQRFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLSQRANADPGHRYA
                     IFIDEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPANVDIIGAMN
                     TADRSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREH
                     TLGHAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQK
                     QDDNLQFVIEKTDDLDTLFGNNHGLRHHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQ
                     QTPLDEQAVNHG"
     gene            1383..2669
                     /locus_tag="QBH27_004152"
     CDS             1383..2669
                     /locus_tag="QBH27_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDQ7016400.1"
                     /translation="MGEVISVFEYDLLGSDKAASVGAKLVPPLVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIETQQATLQAQRMPLLEIFIHQFLHSVSQLLKQGLRSDYVSEQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVIPQAATYSLVKHGSNDCFKLRPDLLIQSRRPVQTK
                     LVMDTKWKLINSNSQKTTQYGLAQADFYQMFAYGQKYLNGIGEMYLIYPAHDQFNQPI
                     QQHFAFSKTLKLWVVPYQITANRGERMMWVKDVSSI"
     gene            2779..4518
                     /locus_tag="QBH27_004153"
     CDS             2779..4518
                     /locus_tag="QBH27_004153"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     endonuclease"
                     /protein_id="HDQ7016401.1"
                     /translation="MKMGKIAVTPNNDKAASHSKDENLATNTIKKNVKPKYGDAAFLE
                     YTRLIVNHPNYFGMPDPFGEKGEIQWEAPSNRASGKFKHTHQRRYEWWKNKARSIGID
                     PDTEKAWISKTAKLIHPLGVKPCKKCGKEMELSYSYPNEHFFSRVRKLNYIDETFELS
                     QNEHIVDLLTRLDDRFGERIYLDLPHLFSTKSITIPDISSNLEAWIEYLKEQYIPQES
                     RMLSPGAMANPPDRFDGFHSFNRCCRSIADKGRTKENLKSYVTDRRVFEYWVDGDWVA
                     ADRLMGQVRTNNIFINEECLNAGNGGLHPTPCQADHIGPISLGFSHRPQFQLLCKSCN
                     SAKNNRMYLSDIISLLEAENEGHTVISWFAEEVWNRLKHSVDDSEKALRLSKILRDNR
                     HTYMNLLKKIMDEGYYTFLASLLHLEVANYNPIFEGLCISNHLTHYKSLKKIKRESKY
                     AAVQKTRRIRIAFTSLNDYHRKENRNAFIVSNELSEKFFSEAMDNLKSLSEITSCLDE
                     KISGIISENSDSKNEFRTIITDLREIVTNNKEKFNLILKYLISGMSEIGKELESYWEN
                     DRYVRSIPEEFIE"
     gene            complement(4531..5721)
                     /locus_tag="QBH27_004154"
     CDS             complement(4531..5721)
                     /locus_tag="QBH27_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDQ7016402.1"
                     /translation="MTKSETFMIPNHKAAKLSELDMMIVNSVPPGGNWKNIPLDVPSK
                     RIEQIRDSYAQGKGSRSTYYGRLLPDMPAYTINTYFNRPGNGCHIHYEQDRVLSQREA
                     ARLQSFPDDFIFFGGQTAINTQIGNAVPPFLAFLIAKEIEKAIGNTGYYIDLFSGAGG
                     LGLGFKWAGWTPLLANDIEEKYLQTYSNNVHKEVLCGSISDNETFSKIADKISGFKKL
                     YFDKQLWILGGPPCQGFSTAGNARTMDDPRNSLFMHYKSLLNEIKPNGFIFENVAGLL
                     NMEKGKVFERVKEEFSSTMKTMNGWILNSEHYAIPQRRKRVILVGSNDPLFSIEPPQK
                     LTEDKESWVSVKDALSDLPPLQHGEDGSGKYYIHHPENDYQLFMRGNITPSEYYERNI
                     KPSL"
     gene            complement(5714..7354)
                     /locus_tag="QBH27_004155"
     CDS             complement(5714..7354)
                     /locus_tag="QBH27_004155"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ7016403.1"
                     /translation="MEEIFYMKHIHLINSLSLDETTKFTKKATGKYYTDPKIALLMIE
                     KLLPLINSCDKKSYNVADPFSGDGRLITLLIKQWMINGFPDVEWNVYLFDIENTGLTY
                     AKNALSELKLAGANINITIKNSDVFYEFKKYVDYFDCVITNPPWENIKPDSRELDFFE
                     PSMKSMYIDSLREFDDYLSRVLPYSQPKRKFAGWGTNLSRVGAELSLEICNKNGLVAI
                     VMPASFFADEQSYILREKFFNSGRIDCINYYPAEAKLFGGADVSSCSFIFNKGESLND
                     NIQLSVYDKNLNIKSLGFFDLSSIDSQYLSIPVSQGVHAVHLLRKLQEGYPTWGSLEK
                     NGEIWAGREIDETGSSDWTQKSGGGLLFIKGKMIGRYNFHNEKSLRITKKIDKVLSNS
                     NFVRIAWRDISRPSQKRRMIATIIPPNSLAGNSLGVVYYKSGSQDSLFSLLGIINSLC
                     FEFQLRSFLATGHVSLSALRKTAIPSEKILLQHSELKQLVISCIEGCCDAELKIEAYV
                     AKNIYKLDLNEFNKLLSSFDKIELAEKESLLRIFQHYD"
     gene            complement(8127..8303)
                     /locus_tag="QBH27_004156"
                     /pseudo
     CDS             complement(8127..8303)
                     /locus_tag="QBH27_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2692 a         1763 c         1576 g         2567 t
ORIGIN      
        1 cgagaacaag atcatcctac attcaccaac taacgtgatc ttctttggcc cccctggcac
       61 tggcaaaacc ttcaggttgc agcaaaaaat gaaagagtac acttctcatg ctgttccagc
      121 tgatcgtgat gcctggctgg attctcgcct tgaatcgttg aattggatgc aggttataac
      181 gctggtgctg ctcgatcttg ggaaacgagc caaagttcgc caaattattg aacatatgtg
      241 gtttcaacgt aaggcattat taaacggtcg taatggcaac ctatcgaata cagcctgggc
      301 agctttgcaa tcctatacag ttcccgagtc gttaaccgtt gattataaga atcggcgtga
      361 gcctgccgta tttaacaaaa cagataacag cgaatggttg ctagttgatt cacagctcga
      421 gcaagtggag gatttggtag agctctacgc cgaacttaaa cgaggcccta aatctgccga
      481 agccatccag cgttttgcgg tggttacgtt ccaccaatct tacggctatg aagaatttat
      541 tgaaggtata cgcgctcgct ctgacgagag tggcaatatc tcttatccca ttgagccggg
      601 tatctttatg cgcctttccc aacgtgccaa tgccgatcca ggacatcgct acgccatttt
      661 cattgatgag atcaatcgcg gtaacatatc caagatcttt ggtgaactaa tctcactcat
      721 tgaagtagac aagcgtgcag gcatgcccaa tgcaatgagc ctacaactgg cttatagcgg
      781 tgatcacttc agcgtacccg ccaatgtcga tatcatcgga gccatgaata cagcggaccg
      841 ttctttagct ctgatggaca cggctttgcg ccgtcgcttt gactttgtcg aaatgatgcc
      901 cgatctctct ttactgagtg gagctaaggt gaaaggcata gagctcgagt cgttgttaga
      961 gaaactcaat agccgcatcg aggctcttta cgatcgtgaa catacgctgg ggcatgcgtt
     1021 ctttatgccg gtaaaaaatg cactcgatgc cggtgatgaa gaagctgcgt ttaaacaatt
     1081 gaagatcgca ttccagaaaa agatcattcc gcttttacag gaatactttt tcgatgactg
     1141 gaacaagatc cggttggtgc tggcagacaa tcaaaagcaa gacgacaacc tgcaattcgt
     1201 gattgagaaa accgacgatc tcgatacgct ttttggtaac aaccatggtt tacgacacca
     1261 tgatcagcaa tcaacagctt acgaactcaa agatttcgat caagagatct ggaatattcc
     1321 acaggcttat cgttcaattt atcagcccca gcagactccc cttgatgagc aggctgtaaa
     1381 tcatgggtga agttatctcc gtttttgaat atgacctatt ggggagtgac aaagcggctt
     1441 cagttggcgc aaaactggtg cctccactgg tctttaatta tttagaagcg cttagcttgg
     1501 caagtaatca agggagccag ttcctcaaac tcacctctcg ctcaggcttc aaattgcttc
     1561 aggttcaaaa ctacgcgggg atgctttcaa cacctcacgg tttccagctt gagatactgc
     1621 ccaaggttgg taaaaacctc acagcggcta atgcacgtca aacattactg acgatgctaa
     1681 gccatttgcc tgggtttcga catattgaaa ctcagcaagc cactcttcag gctcagcgta
     1741 tgcctttgct tgagattttt attcaccagt ttttgcatag cgttagccaa ttacttaaac
     1801 aaggtttacg ctccgactat gtgagcgagc aaggtaatct ggcttttatg aagggcaagc
     1861 tgatgctttc tgcacagctg cgacataacg cggtgaatcg ccataagttt tgtgtcgatt
     1921 atgatgacta catgcctgat tgtgcagcca atcggctctt acactccgcg ctagataagc
     1981 ttcttagtct aaagctgtca tcagagaatc aacgctggct ttacgaactg cgctttgcgt
     2041 ttgacggtat tccacttagc cgggatattg aacgtgatat aaacaactta cgcctcgagc
     2101 gtggtatggc tcattacaac gagccaatgg cgtgggcgca attgatcctg agaggaatga
     2161 gtccgagtgc tttgcaggga aacaccaaag cgatatcact tttgttccct atggaagcgg
     2221 tatttgaatc ctttgtggca cagaccctac cctacgaatt accttctcat ctcaaagtaa
     2281 taccacaagc ggctacttat tctcttgtga agcacgggtc taacgattgc tttaaacttc
     2341 ggccggattt gttgattcag tcacgtcgac cggtacaaac caaattggtg atggacacaa
     2401 aatggaagct aataaatagc aactcccaaa aaacaacaca gtatgggcta gcgcaagccg
     2461 acttctatca aatgtttgcc tacggtcaaa aatacctaaa tgggattggc gaaatgtact
     2521 taatctatcc tgcgcatgat caatttaacc aaccgataca gcagcatttt gctttctcta
     2581 aaacattaaa actatgggtt gttccgtacc agataacagc aaaccgcggg gagaggatga
     2641 tgtgggtgaa ggatgtgtcc agtatataaa tttctagcca agctggcgat gaaccagtcc
     2701 tctaaaaaat gggtataatc atagtttatt tactaattaa attaatataa agtaattact
     2761 ttattatatg gagaatttat gaaaatggga aaaattgcag ttactcctaa taatgataaa
     2821 gcagccagcc actctaaaga tgaaaattta gccacaaata caattaagaa aaacgtaaaa
     2881 cctaaatatg gcgatgcggc atttttagag tacactcgac taatagttaa ccacccgaat
     2941 tattttggaa tgccagatcc ctttggggaa aaaggtgaga ttcaatggga agctccatca
     3001 aatagggcat cggggaaatt taaacacact catcaacggc gctatgaatg gtggaaaaat
     3061 aaagccagaa gtattgggat agaccctgac actgaaaaag catggattag taaaacagct
     3121 aaactaattc atccacttgg agttaaacca tgcaagaaat gcggtaaaga aatggaatta
     3181 tcttactcct acccaaatga gcatttcttt tctcgggtta ggaaacttaa ttatatagat
     3241 gagacttttg agctatctca aaatgaacac atagttgatt tgttgactcg tttagatgat
     3301 agatttggtg aaagaatata tttagattta ccacatttat tttctacaaa atccatcacg
     3361 attccagata tttcaagcaa tctcgaagca tggattgagt atctaaaaga acaatacata
     3421 cctcaagaat cacgtatgct tagccctgga gccatggcaa atcctccaga tcgttttgat
     3481 ggatttcatt catttaaccg ctgttgtcgc tctattgctg ataaaggaag gaccaaagaa
     3541 aatcttaaat cttatgttac tgacagaaga gttttcgaat attgggtgga tggtgattgg
     3601 gtagctgcag acaggctaat gggccaagtt agaacaaata atatatttat taacgaggaa
     3661 tgtttgaatg ccggaaatgg tggtttacat ccaactcctt gccaagcaga tcacattggc
     3721 ccaatctcac tagggttttc ccatcgccca caatttcaac tactttgcaa atcatgtaat
     3781 agtgccaaaa acaatcgtat gtatttaagt gatataatct ctttattaga agctgagaat
     3841 gaaggtcata cggttatttc atggttcgca gaagaagtat ggaatcgact aaaacactct
     3901 gtagatgata gtgaaaaagc cttaagacta agtaaaatac tacgtgataa tagacataca
     3961 tatatgaatc ttcttaaaaa aataatggat gaaggttatt atactttcct tgcaagtctt
     4021 ttacatcttg aggtagccaa ttataaccca atatttgagg gattatgcat ttcaaatcat
     4081 ttaactcatt ataaatcatt aaaaaaaata aaacgtgaat caaaatatgc tgctgtgcaa
     4141 aaaacaagac gaatcagaat agcatttact tcattgaatg attatcatag aaaagaaaat
     4201 cggaacgcat ttattgtatc aaatgaatta tctgagaagt ttttctctga agctatggat
     4261 aatttaaaat cattaagtga aattacatct tgcttagatg aaaaaatatc cggaataata
     4321 tctgagaatt cagattcaaa aaatgaattt cgaacaatca ttacagatct acgagaaata
     4381 gtgacaaaca ataaagaaaa attcaacttg atattaaaat atctaatatc agggatgagc
     4441 gaaataggaa aagaactgga atcatattgg gaaaatgatc gttatgttcg ctcaatccca
     4501 gaagaattta tagagtaaga catagccctc ttataaagag ggctttatat tcctttcata
     4561 atattctgaa ggagtaatat tacctctcat gaataattga tagtcatttt cggggtgatg
     4621 gatataatat ttaccacttc catcttcacc atgttgcaaa ggaggcagat cagataatgc
     4681 atcctttaca gatacccaag attccttatc ttctgttaat ttttgtggag gttcaattga
     4741 aaataaaggg tcgtttgaac caaccaatat aacccttttt cggcgctgag ggattgcata
     4801 atgttcagag tttaatatcc atccattcat agttttcatg gttgatgaga attcttcttt
     4861 gactctttca aagaccttac ctttttccat attcaacaaa ccagcaacat tttcaaaaat
     4921 aaaaccgttt ggttttatct catttaagag agatttataa tgcataaaaa gtgaatttct
     4981 tggatcatcc attgttctgg catttcctgc cgtagaaaat ccctggcatg ggggtccacc
     5041 aagtatccat aattgtttat caaaatataa tttcttaaat cctgatattt tatctgctat
     5101 ttttgaaaaa gtttcattat cagaaatgct gccacataaa acttctttgt gaacattgtt
     5161 tgaatatgtt tgtagatatt tttcttcaat atcattcgcc aataatggag tccatccagc
     5221 ccatttgaat cctagtccca gccctccagc tccggaaaat aaatcgatat aataaccagt
     5281 attacctatc gccttctcta tttctttcgc aatcagaaaa gctaaaaatg ggggcactgc
     5341 attaccgatt tgggtattaa ttgctgtttg acctccgaag aaaataaaat catccgggaa
     5401 cgattgtagt cttgcagctt ctctctggga tagaacacga tcttgctcat agtgaatatg
     5461 gcaaccattc ccaggtcggt taaaatatgt attgatagta tatgcaggca tatctggaag
     5521 taatctaccg tagtatgttg aacggcttcc ttttccttgt gcataactat ccctaatttg
     5581 ctctattctt ttagacggta catctaaagg aatatttttc cagttgccac ctggcggtac
     5641 tgagttaaca atcatcatat ccaattcact tagctttgct gccttgtgat tgggaatcat
     5701 aaatgtttca cttttagtca taatgttgaa aaatcctcaa taaactttct ttttcagcta
     5761 actcaatctt atcaaatgaa gacagaagct tattaaattc attcaaatcc aatttgtaaa
     5821 tatttttggc aacataagcc tcaattttca actcagcatc acaacatccc tctatgcaag
     5881 aaatcacaag ttgttttaat tcagaatgtt gtaataatat tttttcagac ggtattgcag
     5941 tttttcgtaa ggctgacaat gatacatgac cagtagctaa aaagcttctt aattgaaatt
     6001 caaaacaaag ggagtttata ataccaagca aactaaagag cgaatcttgc gaaccacttt
     6061 tataatagac cacccctaat gaattccctg ccaaagagtt aggaggtatt atcgttgcaa
     6121 tcattcgcct tttttgactc gggcgtgaaa tgtcacgcca tgcaatcctt acgaaattag
     6181 aattggatag aaccttatca atttttttcg ttattcttaa gctcttctca ttatgaaaat
     6241 tatacctccc aatcattttt cctttaatga ataataatcc gccgccactt ttttgtgtcc
     6301 agtcactact accagtttca tctatttctc ttcctgccca tatctctcca tttttttcca
     6361 gactacccca tgttgggtat ccctcttgca gctttcttaa caaatgaacg gcatgaacac
     6421 cttgagatac cggaatgcta agatattgag aatctatact agacaaatca aaaaaaccta
     6481 gtgatttaat atttaagttt ttatcataca cactaagttg aatattatca ttaagggatt
     6541 cacctttatt aaaaataaaa gaacatgaag aaacatcagc accgccgaat aatttagcct
     6601 cagctggata ataatttatg caatcaatcc tccctgaatt aaaaaacttt tcacgtaata
     6661 tgtacgattg ttcatctgcg aaaaaagatg caggcataac tatagctacc aatccatttt
     6721 tattacatat ttctaagcta agttctgcac caacacgtga aagattagtc ccccacccag
     6781 caaatttacg cttcggctga gaatatggca atactcttga caaataatca tcaaactcac
     6841 gaagagagtc tatatacatc gatttcatag atggttcaaa aaaatcgagc tctctagaat
     6901 ctggttttat attctcccaa ggcggattgg ttataacaca atcaaaataa tcaacatatt
     6961 ttttaaattc ataaaaaaca tcactatttt ttatagtaat gttaatattt gcacctgcta
     7021 acttaagttc agacaacgca tttttagcat aagtgaggcc tgtattttct atatcgaaca
     7081 gataaacatt ccactctaca tctggaaaac catttatcat ccattgttta ataagcaatg
     7141 taataagtcg accatcccct gaaaatggat ctgcgacatt ataacttttt ttatcacagg
     7201 aatttatcaa tggtaataac ttctctatca tcaaaagagc tatttttgga tctgtatagt
     7261 atttacctgt cgctttttta gtgaattttg ttgtctcgtc caaggacagt gaattaatta
     7321 aatggatatg tttcatgtaa aatatctctt ccattaataa tatggattat atcatttaac
     7381 ttgtgaacac tcaacaataa ctgtacataa aaacagtcat taaagatttc ccctagagtg
     7441 agcatcgcta taaaaattta aattaatttt atcctgctcc gaaagaaaca gttatcattt
     7501 attccataaa gaataacttg ctttataatc actttaaata tcgatatcaa ttttaatact
     7561 atggttactt accatatcaa taaatagctt aacccgttat ctcgataccc cttaagaacc
     7621 ccttccgcaa ctgcaacata atctacgctc atccacatgc ctggtgcgta ttaccgtgaa
     7681 ggagatcggt gagttattga gtgatctcct ttcatgaaaa tacgcacctg gctttaactg
     7741 ttcctgatat aagttgacag ttttctgtcc gtaaaacggt tgatgaactt catcgggcgt
     7801 tttgtatttc aatgccaaat gtgaccgctc atggttataa attgccacga attcttttac
     7861 catttaccgg ccctacgtca gctctgcagg acgcgagagt aaaaactcat tttcaggatt
     7921 ccgtttatcc actcagcctg cgcattctgg tggcagttat aaccattagt cattgaacag
     7981 gttattgata aataattaaa aaactcagtt ttctggctcg cctgctcaag ctgttcttca
     8041 agttccctga ttctttgctc gggtgtcagc gggacagtcg ccagagccac aggcagtctc
     8101 ctctttacct ggtcaggaag tccggggctc cagtcaagcc ggccatattt acgcaaccag
     8161 acaagaacgg tggagcgccc ctgaatgcca taactgcagc cggcctgttt ataagtcatc
     8221 tcgccttttt caaccttctc aacaacggct attttaaagg atagaggata atcacgttga
     8281 gtgcgtttga acccagacat catcacgttc tccgggttta agtcaggaac gtgtcatagc
     8341 tattcaggac gggtcaccga cacaaaaaaa gtcgcccttg agcgactcga tttgcatacg
     8401 gtatggtgcg aaggccggac tcaaacatgc gatttaacct catgatttaa aattgttaat
     8461 aataaacaac accatcctat accaacacag ataccaacac gaaaaatcac tggttttcgg
     8521 catcgaacca ggacaatcag gtcctgattg agataccgag ctctgttact cgtcatcttc
     8581 ctcgacaggc tccattgc
//
LOCUS       DAOFSV010000172         8556 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.172, whole genome shotgun sequence.
ACCESSION   DAOFSV010000172 DAOFSV010000000
VERSION     DAOFSV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.172"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(76..642)
                     /locus_tag="QBH27_004157"
     CDS             complement(76..642)
                     /locus_tag="QBH27_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ7016404.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            1120..1908
                     /gene="fabI"
                     /locus_tag="QBH27_004158"
     CDS             1120..1908
                     /gene="fabI"
                     /locus_tag="QBH27_004158"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDQ7016405.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            2053..3180
                     /locus_tag="QBH27_004159"
     CDS             2053..3180
                     /locus_tag="QBH27_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDQ7016406.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3248..5182
                     /gene="rnb"
                     /locus_tag="QBH27_004160"
     CDS             3248..5182
                     /gene="rnb"
                     /locus_tag="QBH27_004160"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDQ7016407.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5259..5362)
                     /locus_tag="QBH27_004161"
                     /pseudo
     CDS             complement(5259..5362)
                     /locus_tag="QBH27_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5416..7401
                     /gene="pdeR"
                     /locus_tag="QBH27_004162"
     CDS             5416..7401
                     /gene="pdeR"
                     /locus_tag="QBH27_004162"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDQ7016408.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            7549..7722
                     /locus_tag="QBH27_004163"
     CDS             7549..7722
                     /locus_tag="QBH27_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016409.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7811..>8556
                     /locus_tag="QBH27_004164"
     CDS             7811..>8556
                     /locus_tag="QBH27_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7016410.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVD"
BASE COUNT         2127 a         2143 c         2066 g         2220 t
ORIGIN      
        1 gaacccgcat cgcgaaatga atttcgcaat gaaataaggg ggattaatat cccccgctat
       61 tggttaattc tgttcttatt tatttatcaa catactaaca atatcctgat atatttcctg
      121 cagatgttgt ggatcgcttt tttgcggtgt taaacgacga taaaccgtac cttccatcat
      181 ggtagcaaac acttcaacgc agcaacgaat gtgctcgtcg ctaagatggg gatgcattct
      241 tttcatatgt gcacaaccgt tagcgaacat tctggcgtca gcttcttcaa gcattgccat
      301 aacccgcggg ttgcgtgtgg cttctgcagc cacttccagc atcagcgcgt cgtcgtcctc
      361 atttaaggat ttacgcaagg caaggacttc cgggaagtga tcggtacgcg cgtcaatgtc
      421 catttgggca atacgaaagt cgatgatacg gcgaaccatc tcttcaataa tggcgtcttt
      481 gttggcgaag taacgatata tctgacccac gctaagctga gcttcgctgg caatttgcga
      541 catactggcg gcatgaaatc cgcagcgacg aaaacaccga cgggcggcgt taatgatttc
      601 atcctgacgc tgtttgtggc gtatctccag cttgctggtc atttctgtct cctggcctat
      661 cacttggtga gaatgatcat tctcattttt gctaattata cgtttctcca aagtaatgga
      721 aagtattttt atcttattca tgatgcatat tctgataatg aagagattgt ggagattctg
      781 tgaagatagt tgacaatatt aagttggagt caattcatca gaaagacagc gacttgttgt
      841 cgccagttct gttttcatca ttaaacttat aggagctaat acgaatagtt tcaaagtcag
      901 attctataag atatttcccc aacctccccc ttgcaggcgt caggagtaga taacgcaaaa
      961 tgctctgtac cgcagcttct ctccgctaca gaaagcgcaa ctatagccac ccacagccag
     1021 gttgattata ataaccgttt atcagttcgt actgtttact aaaacgacga atcgcctgat
     1081 tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct ttccggtaag
     1141 cgcattctgg taaccggtgt tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg
     1201 atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact gaaaggccgc
     1261 gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga tgttgccgaa
     1321 gataccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa atttgacggt
     1381 ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta tgttaacgcc
     1441 gttactcgtg aaggcttcaa aattgcccac gacatcagct cctacagctt cgttgcaatg
     1501 gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct ttcctacctt
     1561 ggcgctgagc gcgctatccc gaattacaac gttatgggtc tggcaaaagc gtctctggaa
     1621 gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc
     1681 tctgctggtc cgatccgtac tctggcggct tccggtatca aagacttccg caaaatgctg
     1741 gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga tgtgggtaac
     1801 tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt ggtccacgtt
     1861 gacggcggtt tcagcatcgc tgcaatgaac gaactcgaac tgaaataatc gttctgttgg
     1921 taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg atatttgtta
     1981 tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt cctttacgac
     2041 caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta tcatgaaacg
     2101 caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc aaaggtcagc
     2161 gatccctttc tgctcgacgt gatccttgat gaagaaacgc tggccccctt cctttcatgg
     2221 ctggtccctg cgcgcgttct tgcagtggag ttgttccctg acccgcttac cgtgactcgt
     2281 tcacagacct tcaccgctta tgaacgcttg tcgacggccc tgacggttgc tcaggtttgc
     2341 ggcgtccaac ggttatgtaa ctactattct gcgcgactta cgccgctccc cgggcctgat
     2401 tcctccaggg aaagtaatca tcggttggca caaatcacgc aatatgcccg ccaactggct
     2461 agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt tggtcttact
     2521 gtctgtgact gtgtgatcat taaccaaatc attggtttta ttggctttca ggcgcggacc
     2581 attgcgacat ttcaggctta tctcgggcat ccagtacgct ggttacccgg gctggagata
     2641 caaaactacg ccgacgcgtc actgtttgct gatgaatcat tacgctggcg aagcagctat
     2701 gaggtggaaa aactccctga agagcacaca aaaagttcaa ctgcagaact ttgccaactg
     2761 gccaacacac tctctctcca ccctatttca ctttcccttc tcgaaaagtt gttaaacagc
     2821 acacgggtca atacacagcc ggataatcag cttgcggcgt tgttatgcgc gcggataaat
     2881 ggcagtcctg cttgttttgc cgcctgtatg aattcatcaa atgaatataa aaaaatcagc
     2941 ccccttctgc gcaagggcga aaatgaaatt aaccgatggg ctgaccgtca ttctgttgag
     3001 cgcgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt tagcgccgcc
     3061 cagttcagcc cattactcga acacgaaaaa tcatcaacgc agattattaa tctgctagta
     3121 tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga gacatattaa
     3181 ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata
     3241 gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac tgcattccca
     3301 gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct tcctggaagt
     3361 cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca tgcatggcga
     3421 ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc cagaagaact
     3481 ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg accgtctggc
     3541 catcgttcct gaccatccac tcttaaaaga cgccattcct tgccgcgcag cccgtggcct
     3601 gaaccacgag tttaaagaag gtgactgggc ggttgccgaa atgcgccgtc atccgctgaa
     3661 aggcgatcgt tctttctatg ctgaactgac acaatacatc acttttggtg acgatcactt
     3721 tgtaccgtgg tgggttaccc ttgcgcgtca taatctggaa aaagaagcac cagacggcgt
     3781 tgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgactgcgc tggattttgt
     3841 caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcca aggcgttacc
     3901 ggatggcaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg
     3961 cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc ctggcttcaa
     4021 catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg ccaatgaagt
     4081 ccgcccggta ctggcatgcc gcatgacgct ctccactgat ggcaccattg aagataatat
     4141 cgaattcttt gccgccacca tcgaatccaa agcgaagctg gtgtatgacc aggtttctga
     4201 ctggctggaa aataccggtg actggcagcc tgaaagcgaa gcaattgccg aacaagtccg
     4261 tttgctagca caaatttgcc aacgccgcgg cgagtggcgt cataaccacg cactggtgtt
     4321 taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt
     4381 cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat
     4441 ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg tgcatatggg
     4501 ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt
     4561 cgatgctgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg aactggatgc
     4621 gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg ctgaaattag
     4681 cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccactt ggacttcgcc
     4741 gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga
     4801 aactgcgacg cgtccacagg atgaaatcac tgtccaaatg gccgagcgtc gccgtctcaa
     4861 ccggatggca gaacgtgatg ttggcgactg gttatacgca cgcttcctga aagacaaagc
     4921 cgggaccgac acccgtttcg cggcggaaat tgtcgatatc agccgtggcg gcatgcgtgt
     4981 tcgtttggtt gataacggcg ctatcgcctt tattccagca cctttcttac acgctgtgcg
     5041 cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg aaacggctta
     5101 caaagtgact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa cccgcagcat
     5161 tattgcgcgc ccagtcgcgt aatctccttt cacggcccat tcctcatgga tgggccgttt
     5221 atttccccgc tcaaccttcg tcatatcccg gcagattttt aactgacttt cgtttgaaaa
     5281 ctggcgtttt tccaaaactg gattacaaat aaatatataa aaaaacaatg tgtttagccg
     5341 ccgttaattt catgtaagtc atgttgttcc ttttatttaa ctgcggactc cgctgttaac
     5401 cggaggatat gcatcatgaa aaccgttagg gagtccacaa cgttgtacaa ctttctcgga
     5461 tcgcacaatc catactggcg gttgacggaa agcagcgatg ttttgcgctt ttctaccacc
     5521 gaaatcacag aacccgatcg tattttgcaa ctatctgccg aacaggctgc tcgcatcagg
     5581 gaaatgacgg tcatcacctc cagcctgatg atgagtctga ccgtcgatga aagcgatctt
     5641 tctgtgcatc tggtaggacg aaaaatcaat aaacgagaat ggggaggcaa cgcgtctgca
     5701 tggcatgaca caccggcggt tgctcgtgat ttatcacatg ggctttcctt tgctgagcag
     5761 gtagtttctg aagcacattc cgcaatagtg attctcgaca gccgggggaa tatccagcgc
     5821 tttaatcggt tatgtgaaga ttacacaggc ttgaaagaac acgacgtcat tgggcaaagc
     5881 gtgtttaaac tgtttatgag tcgtcgtgaa gctgcggcat ccaggcgcaa taaccgtgta
     5941 ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga taccaacacg taaaggccag
     6001 cggctgtttc tgtttcgcaa taaatttgtc cacagcggca gtggcaaaaa cgagattttt
     6061 ttaatctgtt ccggcaccga cattaccgaa gagcgccgcg ctcaggagcg actgcgtatt
     6121 ctggcaaata ccgacagtat caccggactg ccgaatcgta acgcaatgca ggagttaatc
     6181 gatcacgcta ttaatcaggc agataacaat aaagttgggg ttgtgtatct tgatttggat
     6241 aattttaaaa aggtcaacga cgcctatggg catttgtttg gtgaccagtt attacgcgac
     6301 gtgtcattgg ctattttaag ctgtctcgaa catgaccagg tattggcgcg tccaggtggc
     6361 gatgagtttc tggtactggc atccaatacc tcacaaagcg cgctggaagc aatggcatca
     6421 cgaattttga cccgcttacg gctccccttt cgcattggtt taattgaagt ttataccagc
     6481 tgttcagtag gtatttcact ctctcccgaa catggttcgg acagcgcggc tattattcgt
     6541 cacgccgaca cagcaatgta cacagcgaag gaaggcggac gaggacaatt ttgcgttttt
     6601 accccagaaa tgaatcagag ggtatttgaa tatctctggc tggataccaa cttgcgtaaa
     6661 gcactggaaa acgatcagtt ggttattcac tatcaaccga aaatcacctg gcgtggtgaa
     6721 gtgcgcagtc tggaagcact agtacgttgg cagtcacctg aacgtgggtt gattccaccg
     6781 ttggacttca tttcctacgc cgaagaatca gggctaattg tgcctttagg ccgttgggtg
     6841 attctcgatg tcgtacgcca ggtagcgaag tggcgggata aaggcattaa cctgcgagta
     6901 gcggtaaata tttctgcacg tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag
     6961 gttctccagg aactcaattt tgaatactgc cctatagatg ttgaactgac ggagagctgc
     7021 ctgattgaga atgatgaact ggcactgtct gttattcaac aatttagccg attaggtgcg
     7081 caagttcatc tggacgattt tggtaccggc tactcttcac tttcgcaact ggcgcgcttt
     7141 ccgatcgatg ccatcaaact tgaccaggtt tttgttcggg atattcacaa acagcctgtc
     7201 tcgcaatcac tggtccgggc gatcgtcgct gtggcccagg cattgaatct tcaggtgatc
     7261 gccgaaggtg tagaaagtgc aaaggaagat gcttttttaa ccaagaacgg gatcaatgag
     7321 cgacaaggat ttttgtttgc caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa
     7381 cgctatctga agcgcgcata agtagatgaa tatatatact tagcgctata ttccgtcatt
     7441 atatatcgta ttcccggatg ataattagcc gttgctgtta aacccttctc ttttagcggc
     7501 aatttttgca ttattcattt tccccctttt gctataagga tgtccttgat gtctgaattt
     7561 gatgcccagc gcgttgccga acgtattgat attgtgttag atatccttgt ggcaggcgat
     7621 taccactctg cgatccataa tctcgagata cttaaagcag aactcttgcg ccaggttgct
     7681 gaatctacgc cagacatccc gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt
     7741 ttcctatttg aggatgccgg cgcgtcagta tttttttatt tagtattata acgttataag
     7801 aattacagcg atgaattccc gacaacaaac tattctacag atggtcattg accagggtca
     7861 ggttagcgta accgatctgg caaaagccac tggagtttct gaagttacca ttcgccagga
     7921 tctcaacacc ctcgaaaaac tgagttacct ccgacgtgca catggttttg cagtttcgct
     7981 tgatagtgat gacgtcgaaa cccgtatgat gagcaactat acgctgaagc gtgaactcgc
     8041 cgagtttgcc gcgtcactgg ttcaaccggg cgaaaccatc tttatcgaaa atggtagcag
     8101 caatgccctg ctcgcccgga ctctgggcga gcagaagaaa aatgtcacta tcatcacggt
     8161 cagcagctac atcgcgcatt tgctgaaaga cgcgccttgt gaagtcattt tgctcggtgg
     8221 cgtgtaccag aaaaaaagcg aaagtatggt tggccctttg acacgccagt gcatccaaca
     8281 ggtgcatttc agcaaagcat ttattggtat tgatggctgg caacctgaaa ctgggtttac
     8341 cggtcgcgat atgatgcgta ccgatgtggt caatgccgtg ctggaaaaag agtgcgaggc
     8401 gatagtcctg accgacagct cgaaatttgg tgctgtacat tcatactcca tcggccccgt
     8461 tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc gccagcgatc tgatgcatct
     8521 tgaacaaagt aaactcaccg ttcacgtcgt tgacat
//
LOCUS       DAOFSV010000173         8461 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.173, whole genome shotgun sequence.
ACCESSION   DAOFSV010000173 DAOFSV010000000
VERSION     DAOFSV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.173"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            3..791
                     /locus_tag="QBH27_004165"
     CDS             3..791
                     /locus_tag="QBH27_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HDQ7016411.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            997..1344
                     /locus_tag="QBH27_004166"
     CDS             997..1344
                     /locus_tag="QBH27_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDQ7016412.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1338..2177
                     /gene="sul1"
                     /locus_tag="QBH27_004167"
     CDS             1338..2177
                     /gene="sul1"
                     /locus_tag="QBH27_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDQ7016413.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2305..2508
                     /locus_tag="QBH27_004168"
     CDS             2305..2508
                     /locus_tag="QBH27_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016414.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            2664..3869
                     /gene="chrA"
                     /locus_tag="QBH27_004169"
     CDS             2664..3869
                     /gene="chrA"
                     /locus_tag="QBH27_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HDQ7016415.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            3880..4185
                     /locus_tag="QBH27_004170"
     CDS             3880..4185
                     /locus_tag="QBH27_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7016416.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            4229..4294
                     /locus_tag="QBH27_004171"
                     /pseudo
     CDS             4229..4294
                     /locus_tag="QBH27_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4412..5176
                     /locus_tag="QBH27_004172"
     CDS             4412..5176
                     /locus_tag="QBH27_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HDQ7016417.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(5669..6253)
                     /locus_tag="QBH27_004173"
     CDS             complement(5669..6253)
                     /locus_tag="QBH27_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ7016418.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(6253..7491)
                     /locus_tag="QBH27_004174"
     CDS             complement(6253..7491)
                     /locus_tag="QBH27_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016419.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(7488..8393)
                     /locus_tag="QBH27_004175"
     CDS             complement(7488..8393)
                     /locus_tag="QBH27_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDQ7016420.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1680 a         2494 c         2561 g         1726 t
ORIGIN      
        1 tcatgggtga atttttccct gcacaagttt tcaagcagct gtcccacgct cgcgcggtga
       61 tcgagcgcca tctggctgcg acactggaca caatccacct gttcggatct gcgatcgatg
      121 gagggctgaa gccggacagc gacatagact tgctcgtgac cgtcagcgcc gcacctaacg
      181 attcgctccg gcaggcgcta atgctcgatt tgctgaaagt ctcatcaccg ccaggcgatg
      241 gcggaacatg gcgaccgctg gagctaactg ttgtcgctcg aagcgaagta gtgccttggc
      301 gctatccggc gcggcgtgag cttcagttcg gtgagtggct ccgccacgac atcctttccg
      361 gaacgttcga gcctgccgtt ctggatcacg atcttgcgat tttgctgacc aaggcgaggc
      421 aacacagcct tgcgcttcta ggcccatccg cagccacgtt tttcgagccg gtgccgaagg
      481 agcatttctc caaggcgctt ttcgacacta ttgcccagtg gaatgcagag tcggattgga
      541 agggtgacga gcggaacgtc gttcttgctc ttgctcgcat ttggtacagc gcttcaactg
      601 gtctcattgc tcctaaggac gttgctgccg catgggtatc ggagcgtttg cctgccgagc
      661 atcggcccct catctgcaag gcacgcgcgg cgtacctggg tagcgaggac gacgacctag
      721 caatgcgcgt cgaagagacg gccgcgttcg ttcgatatgc caaagcaacg attgagagaa
      781 tcttgcgttg agcggcatgt gcgaaaagtg catcgacccg cgccgagggc atctgatgcc
      841 taactcggcg ttcaagcgga cgggctgcgc ccgccgctca actatgcgtt agatgcacta
      901 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
      961 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt
     1021 atcgcaatag ttggcgaagt aatcgcaaca tccgcattaa aatctagcga gggctttact
     1081 aagcttgccc cttccgccgt tgtcataatc ggttatggca tcgcatttta ttttctttct
     1141 ctggttctga aatccatccc tgtcggtgtt gcttatgcag tctggtcggg actcggcgtc
     1201 gtcataatta cagccattgc ctggttgctt catgggcaaa agcttgatgc gtggggcttt
     1261 gtaggtatgg ggctcataat tgctgccttt ttgctcgccc gatccccatc gtggaagtcg
     1321 ctgcggaggc cgacgccatg gtgacggtgt tcggcattct gaatctcacc gaggactcct
     1381 tcttcgatga gagccggcgg ctagaccccg ccggcgctgt caccgcggcg atcgaaatgc
     1441 tgcgagtcgg atcagacgtc gtggatgtcg gaccggccgc cagccatccg gacgcgaggc
     1501 ctgtatcgcc ggccgatgag atcagacgta ttgcgccgct cttagacgcc ctgtccgatc
     1561 agatgcaccg tgtttcaatc gacagcttcc aaccggaaac ccagcgctat gcgctcaagc
     1621 gcggcgtggg ctacctgaac gatatccaag gatttcctga ccctgcgctc tatcccgata
     1681 ttgctgaggc ggactgcagg ctggtggtta tgcactcagc gcagcgggat ggcatcgcca
     1741 cccgcaccgg tcaccttcga cccgaagacg cgctcgacga gattgtgcgg ttcttcgagg
     1801 cgcgggtttc cgccttgcga cggagcgggg tcgctgccga ccggctcatc ctcgatccgg
     1861 ggatgggatt tttcttgagc cccgcaccgg aaacatcgct gcacgtgctg tcgaaccttc
     1921 aaaagctgaa gtcggcgttg gggcttccgc tattggtctc ggtgtcgcgg aaatccttct
     1981 tgggcgccac cgttggcctt cctgtaaagg atctgggtcc agcgagcctt gcggcggaac
     2041 ttcacgcgat cggcaatggc gctgactacg tccgcaccca cgcgcctgga gatctgcgaa
     2101 gcgcaatcac cttctcggaa accctcgcga aatttcgcag tcgcgacgcc agagaccgag
     2161 ggttagatca tgcctagcat tcaccttccg gccgcccgct agcggaccct ggtcaggttc
     2221 cgcgaaggtg ggcgcagaca tgctgggctc gtcaggatca aactgcacta tgaggcggcg
     2281 gttcataccg cgccagggga gcgaatggac agcgaggagc ctccgaacgt tcgggtcgcc
     2341 tgctcgggtg atatcgacga ggttgtgcgg ctgatgcacg acgctgcggc gtggatgtcc
     2401 gccaagggaa cgcccgcctg ggacgtcgcg cggatcgacc ggacattcgc ggagaccttc
     2461 gtcctgagat ccgagctcct agggatcgcc tcagaaaacg gaaaataaag cacgctaagc
     2521 cgtaagtaag cgtgctcctg tgaaagccac agctaaaact gcgtagtaca catagaggta
     2581 tttttttcta gatttatatc gaatttcgtt atcgaatttc gcttattatt gatcaactcg
     2641 ttgcagatca tgaaggcgaa agtatgacga ataaccctac cgatgacagc agaccatggt
     2701 cggtctttct tatttttctg cggcttggat tgacatcttt tggcggcccc attgcgcact
     2761 tgggctactt ccgcgccgaa tttgtcacac ggcggcgctg gctctccgaa cggagctatg
     2821 ctgacttggt cgcgctttgt cagttcttgc cagggcctgc aagcagccag gtcggcatag
     2881 cggtaggact gtctcgggct ggatacagcg gggcgctggc tgcttgggct ggcttcacgc
     2941 tgccgtctgc catagccttg atcctttttg cgctcggcat ctccagctat ggcgattacg
     3001 tctcgcaggg cgcgttgcat ggcttaaaag tggtggctgt ggccgtggtc gctcaagcag
     3061 tatggggcat ggcgcgtaac ctatgcacgg atgggctgcg agtcaccatc atggcaattg
     3121 ctacctgcgt cgttttactt gtgccgtccg cgtggggaca ggttggcgtg attgctatcg
     3181 caggcatcgc aggccggtta ttgttcaagc cagcgaaagt tgttgagcat gaccccctac
     3241 ctatcacggt cagtcaccgg gccggcgtgc tttggctctc gctgttcttt gtcttgctga
     3301 ttggcctgcc ggtgttggcc gaactgatgc caagtcaaac catggcaatg gtggattcct
     3361 tctatcgtgt cggatcactg gtgttcggcg gtggtcacgt tgtgctgcca ttactgcaag
     3421 ccgaagtggt gccctccggc tgggtcaaca atgaatcctt tctcgcgggg tacggggcag
     3481 ctcaagcggt gcccggccct ttgttcacgt tcgccgcgtt tcttggtgcc tcgatgaaca
     3541 ccgccccgtc gggctggatc ggcggcattg tgtgtctgct ggctatcttc gcgccctcgt
     3601 tcttgctggt cgtcggatca atgccatttt gggagcgttt gcgccgcaat acaggcatcc
     3661 aagctgcgct ggccgggatc aatgccgctg tagtcggctt gctgctggcc gcgctgtatc
     3721 agcctgtatg gactagcgcc atctttcagc cgcaagactt cggcttggca ttagttgccc
     3781 ttgtcgcact tatgttctgg aagctcccgc cgtggctggt tgtcctcggt agcggtgcag
     3841 ctgggtggct gttgagcgtc gctctgtgag gcacaaaaaa tgactgacaa agacctctac
     3901 ggcggtttga tccgcctgca catccttcac catgcagccg aggaacctgt ctttgggctg
     3961 gggatcatcg aagagctacg ccgacacggc tacgagatga gcgctggcac cgtgtacccg
     4021 atgctgcacg gcctggaaaa aaaaggctat ctgacctcac gccacgaacg caccgggcga
     4081 cgtgaacggc gtgtttatga catcactgaa caaggcagaa ctgcacttgc tgatgcgaaa
     4141 acaaaggtca aggagctgtt cggagagttg gtagaaggtg gttgagtttt tgcttctatg
     4201 tcaggttgag ctatacccta actggattgt cattttcaga agacgactgc accagttgat
     4261 tgggcgtaat ggctgttgtg cagccagctc ctgacagttc aatatcagaa gtgatctgca
     4321 ccaatctcga ctatgctcaa tactcgtgtg ggctctgttg caaaaatcgt gaagcttgag
     4381 catgcttggc ggagattgga cggacggaac gatgacggat ttcaagtggc gccatttcca
     4441 gggtgatgtg atcctgtggg cggtgcgctg gtattgtcgc tatccgatca gctatcgcga
     4501 ccttgaggaa atgctggcgg aacgcggcat ttcggtcgac catacgacga tctatcgctg
     4561 ggtccagtgc tacgccccgg agatggagaa gcggctgcgc tggttctggc ggcgtggctt
     4621 tgatccgagc tggcgcctgg atgaaaccta cgtcaaggtg cggggcaagt ggacctacct
     4681 gtaccgggca gtcgacaagc ggggcgacac gatcgatttc tacctgtcgc cgacccgcag
     4741 cgccaaggca gcgaagcggt tcctgggcaa ggccctgcga ggcctgaagc actgggaaaa
     4801 gcctgccacg ctcaataccg acaaagcgcc gagctatggt gcagcgatca ccgaattgaa
     4861 gcgcgaagga aagctggacc gggagacggc ccaccggcag gtgaagtatc tcaataacgt
     4921 gatcgaggcc gatcacggaa agctcaagat actgatcaag ccggtgcgcg gtttcaaatc
     4981 gatccccacg gcctatgcca cgatcaaggg attcgaagtc atgcgagccc tgcgcaaagg
     5041 acaggctcgc ccctggtgcc tgcagcccgg catcaggggc gaggtgcgcc ttgtggagag
     5101 agcttttggc attgggccct cggcgctgac ggaggccatg ggcatgctca accaccattt
     5161 cgcagcagcc gcctgatcgg cgcagagcga cagcctacct ctgactgccg ccaatctttg
     5221 caacagagcc cgccgtgcta gtctgctcgg tgatggtgga gtgaagccaa cccgcaatcg
     5281 ggttatgaat ctgcatcgcg attcgcaatc agctgtctct tgagcatgtc gaactcctgc
     5341 gatgttatct cgccgtgctc gtgctgctac accaacttgt ccaactcatc cgccacaccg
     5401 ctccccacca cctgcgatgg ccaggttgtt tgctgggcgc attggcggag tatcgtctcg
     5461 acccgggaca cccacaccgg cacaacctta cgggagtgat tcactgtcaa agaatcggcc
     5521 cggtgctctg acgcaagtat cgggatggtc accatttgta agccgtagac ctgagtggtg
     5581 atcaagactt cgataccacc gaccgtaccg gtactaatcg acgacggtcg tgttcgtcgc
     5641 ctgccgcagg gactctgcac acctccgttt acgcatgtgc ctggaggagt tggaaatcgt
     5701 cgtgttcggg aaacattaaa cacaggatgg cagcgatctg agccagcaca tgatcagcta
     5761 gctcaccatc cggatcgacg gcccactgca tcgtcgcgcc agcgatgacc gagtgcagga
     5821 gcaactcagc tgccgcagga gcacctgggg gcagtcgctt gcggatcccc tccaccaccg
     5881 cgcggttccg ctggatcgca agcgtgcgta gctccggcac ctggagctcg taccaggaga
     5941 tgagatagtt caccgagaag tcgttgcgag tgttcatgct ccgaacgagc acctgcaaaa
     6001 attcccagag cccttgcggc cctgcgccta tcggtatcgc attcaggtaa tgccgcacct
     6061 gctcgacgcc gcgctccatc atcctcacca gcagcgtatc gcggttggtg aagcgctgga
     6121 ttaacgctgc gcgggagagc cccacctcct ttgctactcc gctgagcgtg aactctatgg
     6181 gaccgcaacg cttcagcact acggtggcgg cctcgagtac ctcgtcatcg gacttgagct
     6241 tggggcgggg catcagtgtt caccttctgt atgggttggg gcggaggctg tggctgccgc
     6301 cgccattgta gcaaattgaa gacggagcga gagtagagcc acgagccccg caaacacggc
     6361 cgatacaacg aggccaggga gcggaccagc aaggtcgaca aacgcgccgg ccgcaagcat
     6421 aaccatgggc gaggctgaca gcatcaccgc cgagaccgtg ccgagtaccc ggccgagaag
     6481 ttctggcggc gtgcggttgt agatggcagc gttgagaatg ggagagactg agccggtcag
     6541 cagtcccacg agcgcgccca acaacatcag caccggcacg cctggcaact gtgaaagcag
     6601 aagcgagccc accgcagagc cacaaaatgc caccgccagc cagttctgcg ctgatatccg
     6661 ggcgccgacc gacgcatgaa tggcaatgcc aaggagacca ccagccccca tcattgagga
     6721 gaacagcccg agctctgcta cttggcgtcc tgcatctaca aacagcgcag gcatgatgac
     6781 gctgccgttg gcgccaacga tgcccacgaa gatcatcact ataccaaaga gagggcgcag
     6841 caggggttcg ctccagagaa aagcgacgcc ggcgcgcatg gagagagtcg ccgtcgtggt
     6901 catcgtccga gcggcacgcg cgggaagcac ccacgcgccg agcagacctg caaggacgga
     6961 gcagaacgcc gtcagcccga gcgttggcgc agcgccaagc aggccgattg cggccccccc
     7021 aagggccggg ccacctagaa tcgcgacgtt cccgatcacc gctttcagtg acgagacgcg
     7081 ctcaacggag agcccggcga cgtggccgag tttgggcagc tcactgtcct gcgcggccat
     7141 accgggtgcg tcgaacgcgg caccgagcac cacgcaagcg atcagcccag tgttcgagag
     7201 ggcgccaacg gcatcgagca gtgggatgct cgccatggcc acgccgccca ccacacccga
     7261 gatcaatgcg acgggcgcgc gcccgaaccg atcgacgagg ccaccaccaa cccacgcgcc
     7321 gatgatggtc gcgatgacgc tgctagcggc cgtggcgccc gcccaggccg cgctctttgt
     7381 atgagacagg acgaaccatg gaagcgcgag ggccgccacc gcgttgccga tccggaagag
     7441 aaaggtcgcc gcgaacagcg tcgcgagcgg gctatatcga cgttcgctca ttccgctgcg
     7501 gcgagctgcg ccttcgccgc agcgaggtac tcttcgttac ccgagtcgag ggcgaagagt
     7561 gcgtaggtga ccgccccgaa cgcaaggcgc tccgcgatgt ggtgggcgag ccgcggccac
     7621 acccggccac cggccgcttc atacgtgagg aggagcttcg cgagcccctc ttcaccaaag
     7681 accataaggt gcgcggccat gtcgatggca gggtcatcaa cgcgggcctc gctccagtcg
     7741 atcatcccgc tgacgcgctc cgtgttgtcg atgagcacat ggcccacgta gagatcgcca
     7801 tgcaccacca cggagaaatc tggccacgac gaatcgtcgt cgagccagcg ctgccaccgg
     7861 tggaggcgct tgtcgttcac cacgaactcg cgtcggacgc ggtcaacgtc gtcggccacc
     7921 ttctgacggg cctgcgtcgg tgtacggatg agcatccccg catccacggc ggcggaaatg
     7981 gggacggcat gcagggcggc gagcgcggtc gcgaagctct ccgcgaagac ctccgagtcc
     8041 tgcggcacga cccagtcggg cgtggacgaa ccaggctgga tgaccatcgc agtcgagtct
     8101 tcgagcatgg gataggcaac gagctcggcg ttggccacgc gccagtccgg caccgcgaac
     8161 ggcaggcgat tcttgagcat tgccagcacc cgcgcctctg gttcgacctt cgcgcttacc
     8221 tcggctcggc gcgggatgcg cagcacccac cgacgtccat cgtcgacggt ggcgatcacg
     8281 atcctatagt cgagcccaag ctcattgaca gtcagcgggc catggagctt gagcccatgt
     8341 cgggctgcaa gtgcgtacag ttgggaggta tcggcggtcg tgactacggt catgattcac
     8401 tcctgagggc ttgacgggtt tagccaccta aatgtaacag tcacgtcggt tatattcaat
     8461 c
//
LOCUS       DAOFSV010000174         8299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.174, whole genome shotgun sequence.
ACCESSION   DAOFSV010000174 DAOFSV010000000
VERSION     DAOFSV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.174"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            223..786
                     /locus_tag="QBH27_004176"
     CDS             223..786
                     /locus_tag="QBH27_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7016421.1"
                     /translation="MIKSVIAGAVAMAVVSFGANAAASIPQGQGEVSFKGTVVDAPCG
                     IETQSAKQEIDFGQISKSFLQEGGETQPKDLNIKLVNCDITNFKQLQGGAAKKGTVSL
                     TFSGVPAENADDMLQTVGDTNTAIVVTDSSGKRVKFDGATETGASNLINGDNTIHFTA
                     FVKKDNSGKNVAEGAFSAVANFNLTYQ"
     gene            856..1443
                     /gene="papH"
                     /locus_tag="QBH27_004177"
     CDS             856..1443
                     /gene="papH"
                     /locus_tag="QBH27_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapH"
                     /protein_id="HDQ7016422.1"
                     /translation="MRLRFSVPLFFFCCVFVHGVFAGPFPPPGMSLPEYWGEEHVWWD
                     GRAAFHGEVVRPACTLAMEDAWQIIDMGETPVRDLQNGFSGPERKFSLRLRNCEFNSQ
                     GGNLFSDSRIRVTFDGVRGETPDKFNLSGQAKGINLQIADARGNIARAGKVMPAIPLT
                     GNEEALDYTLRIVRNGKKLEAGNYFAVLGFRVDYE"
     gene            1502..4012
                     /gene="papC"
                     /locus_tag="QBH27_004178"
     CDS             1502..4012
                     /gene="papC"
                     /locus_tag="QBH27_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HDQ7016423.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPQSSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLSASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDMFNDGLDSYSLNAGLNSGGGLTSQRQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            4083..4817
                     /locus_tag="QBH27_004179"
     CDS             4083..4817
                     /locus_tag="QBH27_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ7016424.1"
                     /translation="MWKLSMIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGSEKSM
                     TLDISNDNKQLPYLAQAWIENENQEKIIAGPVIATPPVQRLEPGAKSMVRLSTTPDIS
                     KLPQDRESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLIL
                     NKVSGGYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLS
                     YINDYGGRPVLSFICNGSRCSVKKEK"
     gene            4854..5435
                     /locus_tag="QBH27_004180"
     CDS             4854..5435
                     /locus_tag="QBH27_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein papJ"
                     /protein_id="HDQ7016425.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHH
                     YRINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSAAAGAPLMLRLAGCGDG
                     GALLPGKRGVAMTVSGSLVTGHGTGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     DMLAAWRKRAAQEGEYSDARSNPAMLRLSIKYE"
     gene            5445..5981
                     /locus_tag="QBH27_004181"
     CDS             5445..5981
                     /locus_tag="QBH27_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ7016426.1"
                     /translation="MIKSTGALLLFAALSAGQAIASDVAFRGNLLDRPCHVSGDSLNK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNAGRLGIALLDTDGSSLLKPGTSHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            6008..6529
                     /gene="papE"
                     /locus_tag="QBH27_004182"
     CDS             6008..6529
                     /gene="papE"
                     /locus_tag="QBH27_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapE"
                     /protein_id="HDQ7016427.1"
                     /translation="MKKIRGLCLPVMLGAVLMSQHVHAADNLTFKGKLIIPACTVTKA
                     EVDWGNVEIQTLSQNGNHEKEFTVNMQCPYHLGTMKVTITATNTYNNAILVQNTSNTS
                     SDGVLVYLYNSNAGNIGTAITLGTPFTPGKITGNNADRTISLHAKLGYKGNMQSLKAG
                     DFSATATLVASYS"
     gene            6604..7104
                     /gene="papF"
                     /locus_tag="QBH27_004183"
     CDS             6604..7104
                     /gene="papF"
                     /locus_tag="QBH27_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial tip protein PapF"
                     /protein_id="HDQ7016428.1"
                     /translation="MARLSLFISLLLTSVAVLADVQINIRGNVYIPPCTINNGQNIVV
                     DFGNINPEHVDNSRGEVTKTISISCTYKSGSPWIKVTGNAMAGQTNVLATNIANFGIA
                     LYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFTSVPFRNGSRTLNGGDFRTTAS
                     MSMIYN"
     gene            7148..8158
                     /gene="papG-II"
                     /locus_tag="QBH27_004184"
     CDS             7148..8158
                     /gene="papG-II"
                     /locus_tag="QBH27_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151505562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria tip G-adhesin PapG-II"
                     /protein_id="HDQ7016429.1"
                     /translation="MKKWFPALLFSLCVSGESSAWNNIVFYSLGDVNSYQGGNVVITQ
                     RPQFITSWRPGIATVTWNQCNGPGFADGSWAYYREYIAWVVFPKKVMTQNGYPLFIEV
                     HNKGSWSEENTGDNDSYFFLKGYKWDERAFDAGNLCQKPGETTRLTEKFDDIIFKVAL
                     PADLPLGDYSVKIPYTSGMQRHFASYLGARFKIPYNVAKTLPRENEMLFLFKNIGGCR
                     PSAQSLEIKHGDLSINSANNHYAAQTLSVSCDVPANIRFMLLRNTTPTYSHGKKFSVG
                     LGHGWDSIVSVNGVDTGETTMRWYKAGTQNLTIGSRLYGESSKIQPGVLSGSATLLMI
                     LP"
BASE COUNT         2175 a         1730 c         2223 g         2171 t
ORIGIN      
        1 tcggcatttg actaaattat ggcattccgg agtttctgga agataaaaaa agaagccctt
       61 atcagaaagc agacaggtta tatcagtatt ctgtcgataa ataacctgcc ctgaaaatac
      121 gagaatatta tttgtattga tctggttatt aaagataatc gggtcatttt aaattgccag
      181 atatctctgg tgtgttcagt aatgaaaaag aggttgttat ttatgattaa gtcggttatt
      241 gccggtgcgg tagctatggc agtggtgtct tttggtgcaa atgcagctgc atctatccct
      301 caaggacaag gtgaagtaag ttttaaaggg actgttgttg acgctccgtg tggtattgaa
      361 actcagtctg caaaacagga aattgatttt ggtcagatct ctaaatcctt cctgcaagag
      421 gggggagaga ctcaaccgaa agatttgaat attaagcttg tgaattgtga cattactaat
      481 ttcaaacagc ttcaaggcgg ggcagctaaa aaaggtacag tgtcattgac attttcaggt
      541 gtgcctgcag aaaatgcaga tgacatgctg caaacagttg gagatactaa tactgcgatt
      601 gttgttactg attcgagtgg aaaacgggtg aaatttgatg gagccactga gaccggggct
      661 tcaaatctga ttaatggcga taatacaatt cattttactg catttgttaa gaaagataat
      721 agtggcaaga atgtagctga aggtgccttc tcagcagttg cgaatttcaa cctgacttat
      781 cagtaacagt aatacgataa tctggtccgg taaacagcgg aaatattccg ctgtttattt
      841 ctcagggtat ttatcatgag actgcgattc tctgttccac ttttcttttt ttgctgtgtg
      901 tttgttcatg gtgtttttgc cggtccgttt cctcctcccg gcatgtccct tcctgaatac
      961 tggggagaag aacacgtatg gtgggacggc agggctgctt ttcatggtga ggttgtcaga
     1021 cctgcctgta ctctggcgat ggaagacgcc tggcagatta tcgatatggg ggaaaccccg
     1081 gttcgggatt tacagaatgg tttttccgga cctgaaagaa aattcagcct ccggctcagg
     1141 aactgtgaat ttaacagtca gggtgggaac cttttctctg attcccggat aagggtgact
     1201 ttcgatggcg tccggggtga aacgccggat aagtttaatt tatccggtca ggcaaaaggc
     1261 attaatctgc agatagctga tgccagggga aatattgccc gggcagggaa agtaatgcct
     1321 gcaataccat tgacgggtaa tgaagaagcg ctggattaca ccctcagaat tgtgagaaac
     1381 ggaaaaaaac ttgaagccgg aaattatttt gccgtgctgg gattccgggt cgattatgag
     1441 tgagtcactc cggtgagatg tccggttatt tatctttttt gtgaatctgg tgatgcgtgg
     1501 aatgaaagac agaatacctt ttgcagtcaa caatattacc tgtgtgatat tgttgtctct
     1561 gttttgtaac gcagccagtg ccgttgagtt taatacagat gtacttgacg cggcggacaa
     1621 gaaaaatatt gacttcaccc gtttttcaga agccggctat gttctgccgg ggcaatatct
     1681 tctggatgtg attgttaacg ggcaaagtat ttctcccgca tcgttacaga tttcatttgt
     1741 tgaacctcag tcgtcaggag ataaggcaga aaaaaaattg ccgcaggcct gcctgacatc
     1801 agatatggtc agactgatgg ggttaacagc agaatctctg gataaagttg tttactggca
     1861 tgatggtcag tgtgcggatt ttcatgggtt gccgggagtg gatattcgtc ctgataccgg
     1921 agcgggcgta ttacgcatca atatgccgca ggcctggctt gagtattctg atgccacctg
     1981 gctgcctccc tcacgctggg acgacggcat tcccggactg atgctggatt ataacctcaa
     2041 cgggacggtt tcccgtaatt atcagggagg agactctcat cagttcagtt ataacgggac
     2101 tgtggggggg aatctggggc cctggcgcct gcgggctgac tatcagggaa gccaggagca
     2161 gagccgctac aacggggaaa aaacgacaaa cagaaatttc acatggagtc gcttttatct
     2221 gttccgtgcc attccacgat ggcgggcaaa cctgacgctg ggcgagaata atatcaactc
     2281 agatatattc cggtcatgga gttatacggg agccagcctg gaaagcgatg accggatgct
     2341 gccacccaga ctgcgaggct atgcaccgca gattaccggg attgcggaga ctaatgcccg
     2401 tgttgtggtg tcgcagcagg gacgggtgct gtacgactcg atggtccccg cagggccatt
     2461 cagtattcag gacctggaca gttcagttcg cggacgtctt gatgttgagg ttattgaaca
     2521 gaacggacgg aagaaaacct ttcaggtcga tacggcctcg gttccttatc tgacgcgtcc
     2581 gggacaggtc cggtacaaac ttgtctccgg tcgctcccgc ggatacgggc atgagaccga
     2641 agggcctgta tttgcaaccg gagaggcgtc ctgggggctc agtaaccagt ggtcgctgta
     2701 tggcggggct gtgcttgccg gtgattataa tgcactggca gccggtgccg gctgggacct
     2761 gggtgtgccg gggacccttt ccgctgatat cacgcagtca gtagcccgta ttgagggaga
     2821 gagaacgttt cagggaaaat cctggcgtct tagctactcc aaacggtttg ataatgcgga
     2881 tgccgacatt acgttcgccg ggtatcgttt ctcagagcga aactatatga ccatggagca
     2941 gtacctgaac gcccgctacc gtaatgatta cagcagtcgg gaaaaagaga tgtataccgt
     3001 tacgctgaat aaaaacgtgg cggactggaa cacctctttt aacctgcagt acagccgtca
     3061 gacatactgg gacatacgga aaacggacta ttatacggtg agcgtcaacc gctactttaa
     3121 tgttttcgga ctgcagggtg tggcggttgg attgtcagcc tcaaggtcta aatatctggg
     3181 gcgtgataac gattctgctt acctgcgtat atccgtgccg ctggggacgg ggacagcgag
     3241 ctacagtggc agtatgagta atgaccgtta tgtgaatatg gccggctaca ctgacatgtt
     3301 caatgacggt ctggacagct acagcctgaa cgccggcctt aacagtggcg gtggactgac
     3361 atcgcaacgt cagattaatg cctattacag tcatcgtagt ccgctggcaa atttgtccgc
     3421 gaatattgca tccctgcaga aaggatatac gtctttcggc gtcagtgctt ccggtggggc
     3481 aacaattacc ggaaaaggtg cggcgttaca tgcaggggga atgtccggtg gaacacgtct
     3541 tcttgttgac acggatggtg tgggaggtgt accggttgat ggcgggcagg tggtgacaaa
     3601 tcgctgggga acgggcgtgg tgactgacat cagcagttat taccggaata caacctctgt
     3661 tgacctgaag cgcttaccgg atgatgtgga agcaacccgt tctgttgtgg aatcggcgct
     3721 gacagaaggt gccattggtt accggaaatt cagcgtgctt aaagggaaac gtctgtttgc
     3781 aatactgcgt cttgctgatg gctctcagcc cccgtttggt gccagtgtaa ccagtgaaaa
     3841 aggccgggag ctgggcatgg tggccgacga aggccttgcc tggctgagtg gcgtgacgcc
     3901 gggggaaacc ctgtcggtaa actgggatgg aaaaatacag tgtcaggtaa atgtaccgga
     3961 gacagcaata tctgaccagc agttattgct tccctgtacg cctcagaaat aaatgaaagt
     4021 ccggaatatt aacggctgat gaatgcggtt atgccatttt ccggaccagg tgaaattaca
     4081 gaatgtggaa attaagtatg attcgaaaaa agattctgat ggctgccatc cccctgtttg
     4141 ttatatccgg ggcagacgct gctgtttcgc tggacagaac ccgcgcggtg tttgacggga
     4201 gtgagaagtc aatgacgctt gatatctcca atgataacaa acaactgccc tatcttgctc
     4261 aggcatggat agaaaatgaa aatcaggaaa aaattattgc agggccggtt attgccaccc
     4321 ctccggttca gcgccttgag ccgggtgcga aaagcatggt caggctgagt accacaccgg
     4381 atatcagtaa acttcctcag gacagggaat cactgtttta ttttaatctc cgggaaatac
     4441 cgccgaggag tgaaaaggcc aatgtactgc agatagcctt acagaccaaa ataaagcttt
     4501 tttatcgccc ggcagcaatt aaaaccagac caaatgaagt atggcaggac cagttaattc
     4561 tgaacaaagt cagcggtggg tatcgtattg aaaacccgac cccctattat gtcactgtta
     4621 ttggtctggg aggaagtgaa aagcaggcag aggaaggtga gtttgaaacc gtgatgctgt
     4681 ctccccgttc agagcagaca gtaaaatcgg caaattataa taccccttat ctgtcttata
     4741 ttaacgacta tggtggtcgc ccggtactgt cgtttatctg taatggcagc cgttgctctg
     4801 tgaaaaaaga gaaataatgt accgcaataa cggttaaatg cgggtgggat attatggttg
     4861 tgaataaaac aacagcagta ctgtatctta ttgcactgtc gctgagtggt ttcatccata
     4921 ctttcctgcg ggctgaagag cggggtatat acgatgacgt ctttactgca gatgagttgc
     4981 atcattaccg gataaatgaa cgggggggac gcaccggaag cctggcagtc agtggtgcac
     5041 tgctgtcctc accctgcacg ctggtgagta atgaggtgcc gttaagcctc cggccggaga
     5101 atcactctgc ggcagccgga gctcctctga tgctgaggct ggcaggatgt ggggacggtg
     5161 gtgcacttct gcccggaaaa cggggcgttg cgatgacagt ctccggctca ctggtaaccg
     5221 gtcacggaac cggaagtgct ttacttcctg accgtaagct atccggctgt gaccatcttg
     5281 ttatacatga tggtgatacc tttttactgt gccggcctga ccggcggcag gaggatatgc
     5341 tggcagcctg gaggaaaagg gcggcacagg agggtgagta ttctgatgcc aggagcaatc
     5401 cggccatgct gcgtctgagc ataaaatatg agtaagggaa tgtgatgata aaaagcacag
     5461 gcgctctttt actgtttgcc gcactgtctg ccggacaggc aatagcctca gatgtggcat
     5521 tcaggggtaa tctgcttgac agaccctgcc atgtgtccgg tgacagtctg aataaacatg
     5581 tcgtcttcaa aacccgggcg tccagggatt tctggtatcc gcccggacgt tcgccgacag
     5641 agtcgtttgt catcaggctg gaaaactgcc atgcaacagc agttggcaaa attgtgaccc
     5701 tgacctttaa gggaacggaa gaggcggccc tcccgggcca tctgaaggta accggagtga
     5761 atgccggccg gttaggcatt gcactgctgg acaccgatgg cagcagtctg ctgaaacccg
     5821 gcacctccca taacaaaggc cagggagaaa aggttaccgg aaacagtctt gagcttcctt
     5881 tcggcgcgta tgttgtggca acgccggaag ccctgcggac gaagagtgtg gtacccggtg
     5941 attatgaagc aacagccacg tttgaattga catatcgtta gcaaaatttt tgtacaggat
     6001 atttcagatg aaaaagataa gaggtttgtg tcttccggta atgctggggg cagtgttaat
     6061 gtctcagcat gtacatgcag ctgataatct gacctttaaa ggaaaactga ttattcctgc
     6121 ctgtactgta acaaaggcag aggttgactg gggaaatgta gagattcaga ccctgagtca
     6181 aaatggaaat cacgaaaaag agtttactgt gaatatgcag tgtccttatc atttgggaac
     6241 aatgaaggtt acgataacgg caacaaacac ttataacaat gctattttag ttcagaatac
     6301 atcaaacaca tcttctgatg gggtacttgt ttatctttat aacagtaatg caggaaatat
     6361 tgggactgcg ataactttag ggactccatt tacgcccgga aaaatcacag gtaataatgc
     6421 agatagaact atatcacttc atgccaaact tggatataaa gggaatatgc agagtctgaa
     6481 ggctggggat ttttctgcga cagcaacgct ggttgcatca tattcgtaat agtatcaact
     6541 aaaatacgcc gattttatat ctcataaaat aaaatgtttt ctgtaccgct ctccggaggg
     6601 ggaatggctc gtttatcatt atttatatcg ttgcttctga catcggtcgc tgtactggct
     6661 gatgtgcaga ttaacatcag gggaaatgtt tatatccccc catgcaccat taataacggg
     6721 cagaatattg ttgtcgattt tgggaatatt aatcctgagc acgtggacaa ctcacgtggt
     6781 gaggtaacaa aaaccataag catatcctgt acgtataaga gtggctcacc ctggataaag
     6841 gtcacaggta atgcaatggc tgggcagact aatgtactgg caacaaatat agccaatttt
     6901 ggtatagcgt tgtatcaggg aaaaggaatg tcaacacctc ttacattagg taatggttca
     6961 ggaaatggtt acagagtgac agcaggtctg gacacagcac gttcaacgtt cacctttact
     7021 tcagtgccct ttcgtaatgg cagcaggaca ctgaatggcg gggatttccg gaccacggcc
     7081 agtatgagca tgatttataa ctgagtcata cctaaatgaa taactgtaat tacggaagtg
     7141 atttctgatg aaaaaatggt tcccagcttt gttattttcc ttgtgtgtgt ctggtgagtc
     7201 ctctgcatgg aataatattg tcttttactc ccttggagac gttaactctt atcagggagg
     7261 gaatgtggtg attactcaaa ggccacaatt tataacttcg tggcgcccgg gcattgctac
     7321 ggtaacctgg aatcagtgta atggtcctgg gtttgctgat ggttcctggg cttactacag
     7381 ggagtatatt gcgtgggtag tattccccaa aaaggttatg acccaaaatg gatatccctt
     7441 atttattgag gttcataata aaggtagctg gagtgaggag aatactggtg acaatgacag
     7501 ctattttttt ctcaaggggt ataagtggga tgagcgggcc tttgatgcag gtaatttgtg
     7561 tcagaaacca ggagaaacaa ctcgtctgac tgagaaattt gacgatatta tttttaaagt
     7621 cgccttacct gcagatcttc ctttagggga ttattctgtt aaaattccat acacttccgg
     7681 catgcagcgt catttcgcga gttacttggg ggcccgtttt aaaatcccat acaatgtggc
     7741 caaaaccctc ccaagagaga atgaaatgtt attcttattt aagaatatcg gcggatgccg
     7801 tccttctgca cagtctctgg aaataaagca tggtgatctg tctattaata gcgctaataa
     7861 tcattatgcg gctcagactc tttctgtgtc ttgcgatgtg cctgcaaata ttcgttttat
     7921 gctgttaaga aatacaactc cgacatacag ccatggtaag aaattttcgg ttggtctggg
     7981 gcatggctgg gactccattg tttcagttaa cggggtggac acaggagaga caacgatgag
     8041 atggtacaaa gcaggtacac aaaacctgac catcggcagt cgcctctatg gtgaatcttc
     8101 aaagatacaa ccaggagtac tatctggttc agcaacgctg ctcatgatat tgccataaat
     8161 ggtttatccg gagccggata gagtgttgtg gatatctggg atgccccggg aagtcacctt
     8221 tcagccactt catttatgga ttatacgcgg cctgcactga gttacgtcca tgtagctcat
     8281 ctgacgttca gccgcttta
//
LOCUS       DAOFSV010000175         8197 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.175, whole genome shotgun sequence.
ACCESSION   DAOFSV010000175 DAOFSV010000000
VERSION     DAOFSV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.175"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(16..429)
                     /gene="pgaD"
                     /locus_tag="QBH27_004185"
     CDS             complement(16..429)
                     /gene="pgaD"
                     /locus_tag="QBH27_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDQ7016430.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     FWQSEARSRLQFYFLLAVVNAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(431..1756)
                     /gene="pgaC"
                     /locus_tag="QBH27_004186"
     CDS             complement(431..1756)
                     /gene="pgaC"
                     /locus_tag="QBH27_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDQ7016431.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWNQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAAQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(1749..3767)
                     /gene="pgaB"
                     /locus_tag="QBH27_004187"
     CDS             complement(1749..3767)
                     /gene="pgaB"
                     /locus_tag="QBH27_004187"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDQ7016432.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVVISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALADFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLIQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            complement(3776..6199)
                     /gene="pgaA"
                     /locus_tag="QBH27_004188"
     CDS             complement(3776..6199)
                     /gene="pgaA"
                     /locus_tag="QBH27_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDQ7016433.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALFKLKQLKS
                     GAPDKANLLAEAYIYKLTGRYQDELRAMTESLPENASKQQYPTEYVLALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSENERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYRDVISHYQRLKKTGQIIPPWALYWVASAYLKDQQPKKAQS
                     IMTELFYNKETIATDLSDEELADLFYSHLESENYPGALTVTKHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEITTRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKMFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            6785..8152
                     /locus_tag="QBH27_004189"
     CDS             6785..8152
                     /locus_tag="QBH27_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ7016434.1"
                     /translation="MEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCMLIFLALINKVSENTKQRNLFSKKVTLCISLFFVLG
                     GPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
BASE COUNT         2240 a         1782 c         1789 g         2386 t
ORIGIN      
        1 ctttgttttg ggtgtttata cccggactag cgttttttct gaaaccacca tttttatttg
       61 tccctggctg gtgaaatgta cgctcatcct gtggcttttt tgtagttgct gataaagctc
      121 atcaggtatt gctaagctct ctgcatattc ttgcggcgta tattggtatg ctgcatgatg
      181 ctgctgtttt tgaaaacgca gcttattgta caacgcccag acgattaaca cgacggcatt
      241 caccactgcc agcaaaaaat agaactgaag tcggcttctg gcctcgcttt gccagaaata
      301 acccgtcagc agatcaatgg cgaataagaa tatgaataaa gcaaataatg tccacaagat
      361 agttgtggca acataatcaa ccagtaaacg tactggcgat tgtcgggtcg taataattag
      421 attgttcata ttaacctctc agaatcccac gatcgggact tacccaacga gcgcgttgtt
      481 ttttaggcat caacatgact cgtgtaaatg ataccaacgt cgttgccagg cttagcatcc
      541 agaaaataac cgggaaccaa ataatccaga aaagcgatga agccagattg tgctcatagc
      601 gattctcaat caccagactg acaataaatt gcagtaaaca taacgtacac aataatattc
      661 cggcagtatg tgtcgctgcg atatgtgtca attcaatatt taacggtaca cctgcaagtt
      721 gggctgcgta aataatgaaa ccgaccaggc aggtaaaggc ccatattgtc gtcagacagt
      781 attcaaaaaa cagtggccac attcgaaagt tttctttgcg ccacaacctt gtcatatttt
      841 tgaggaatac ttctgcaccg ccctgagccc agcgcaggcg ctgattccac agccctttta
      901 acgtttcagg cattaatatc cagcacagcg cccgtggctc gtaaaaaatc gtccactgat
      961 tcaactgcag cttccagcta atatcaatat cttcggtgat catatcgtca ctccagtaac
     1021 ccacttctgc cagggcgctg cgacgaaatg cggcaataac accggaaacg gtaaatacgt
     1081 ttccatagat acgctgggtc cgcttgatca aaccaataat tgaggaatac tcgccaacct
     1141 gaattttacc caccagggta gaacgtgtgc gaatacgagg attaccggtt acggcaccca
     1201 cacgcgggtt gtacaacatc ggttccacaa tatatgccgc tgcatcgcgg tctaataacg
     1261 cgtcgccatc aatgcacacc agatattcac ttttcgccgc ggcagctccg gttttaagcg
     1321 caatggcttt cccctggttt tgcgccagat gaatgacccg cagacgggga atttgtgcag
     1381 ccatgcgatc caggatggca cgggttttgt ctgttgaacc atcatttacg gcaataactt
     1441 caatgttctc ataacgctgt gctaaagcgg catgtatggt ttcttcaacg tttttctcct
     1501 cattaaaaca gggaatgata atggagatag atggattatc tttcaactgg ggggctggtg
     1561 cgttttctcc ccacggccag tggcgttcac gatagaccca gaaatagacg ccgccaataa
     1621 tccacataat ggacataaaa aacggccaga agaaaacgaa cctcatcatc agttcaccag
     1681 agtgaaagta cgctacgcat aggggtatgc ataacaccag acataatata aaaaacgata
     1741 cgatgcgatt aatcattttt cggataccag gctgttgaaa actcaggacg aataacgtct
     1801 atttcaggtt ggttatgcag aaaattgtcg ggataataac cataattttt cactccattc
     1861 agttgtaata ggctcatcca gtgagcgagt tgttgtgaag aaatagcctg atgctgacca
     1921 tttttctgcc agttttgtgc ctgtaattct aaaattgatt tgtctttagc ctgcgggatg
     1981 cttttcatct gattggtcaa ttgtattaac cattgatcag ccgatttttc tgtgacgcct
     2041 tccatataag gcatagccat aatagcggtc cagtcatagc tttttaggaa atcagcataa
     2101 ttctgtgcaa accaggcttc actttcaggt tgtattaccg gaagtgcaaa aatatttcgt
     2161 gcagttttaa catgtggacc acgaatggct tttacgcgcg cactaagttc taaagtgaag
     2221 tcagctaacg cacgactttt aaagcgggtc cactgtttaa attgctccgg gttttgtcga
     2281 atttcgctca gactcccgct aaagccagct tgctgataag ccgcgatagc cgctgcactg
     2341 gcatcttcat aatctgaaag caaagcatca tcgtggaaca atatgccatc aaaagcagca
     2401 tgtccggcaa gatcatcata taacatacca acttgtgctc tgaccctgtc atcgaaagga
     2461 gagagacggc ggtattgttc aggatgaatt tgtgcttttt tctccccagt tggtaagtat
     2521 tttactcgcg ttaatgtggg atctaaatcc cagcttaata ccggcatcca cgcatagatg
     2581 tttacacctg agcgggtacg caattgccag gcaacccgac taaaaatatc tgctttcatt
     2641 ggtagcaaac gatttggaaa ccagacctct ttaaccagcc catcaccatc gggatcagca
     2701 aatgcttgca aatacacggt agatatttgc atatctttta cccgctgaat tagcacatca
     2761 atattgcgat ccatttgctg gcggttttcg tcataaacgt aatcaagatc gatatgcatt
     2821 acccgttgta gtggtttttc ctgtacggta ataatttgct gggcaaactc ttttaatgag
     2881 ggattattgg cgattaatac tcgcggaatg gaatctaatt gcgacgcatt tgccaaacct
     2941 gattcaaggg tgaagaacat gtcataaccg agttttttta attccgctat tgctatgcca
     3001 ttcgcttcgc cataaggcca aacaaaaacg tgtggattta cctcagcctt tgtacgcagg
     3061 tattccgtca tttttacagc atccaggcga attctttccc ggtattctgc tgcggtttca
     3121 taccgtgcgt ggtcagtaaa atatgcacga tttacatatg caggcaataa gctgccggtg
     3181 gcattagcct gaataccgta gtgagaatcc catgtatgag aagcgacctc aacgagccgg
     3241 gaacgcgcaa cttctcgcac ttgttgccac gtggcaaaat attctcgact gaccatctca
     3301 tcgccaaatt tcacttgttt atccgctgga gtatcgaccc aactgccgac gggagcccat
     3361 acagccggcc actggaaggc ctgaagaatt gggaagacac gggtataaaa actctggtag
     3421 ccgtcatcaa aagtcagcac tactgctttt tccggtagcg gttttcctcc tcgatgtgct
     3481 tcacgaattt gagcaatact gaccggttga taaccgttct cgcgcagcca ggcaaattgt
     3541 tcacgcagtg ctgatgttcg cactgacata aaacgctggt cggcagcttc gtcttcaacg
     3601 ttatgccatg aaatcaccac aaaaccatta tgtggccacg gttgctcggc gagtaaagat
     3661 tcgcgatcct gtggcggtat aaatgatgtt cttgactggc taatgcacgc ggtgagcata
     3721 attatactca ccagcatcag gagatattta tttccattac gtaacatatt tatccttaaa
     3781 atctgaatgt catatcgaat tcaacgtata agttgtgttc tctgtcaccg tcataaggtc
     3841 gtttttccca gcgtagcgtt gcgccagcat caatcacgtc attccagcta atacgttgcc
     3901 cgtagccgag ttgggtgacg acatccgtgc cataatgttt ttgccaggag gcaccaacac
     3961 ctgcgctgaa tatttgctcc cagctatttt catagcttcg ccataacaaa tgacttgcct
     4021 caaatgccgg aacaatatcg aacattttta tagggttgta gtatggggtg tcgtgttctg
     4081 tattttgttc gtaatacaga ttgggtagga aatcgacaat caaatatggt gaagaccaga
     4141 tgcgttcttg accctcaatt gagacttcat gacgctggtt actgtcggaa aaatcagtga
     4201 aagcccagga gacaccgtac ttacgccgct cattttgata ccagcgaaca taagcctgtg
     4261 cgctgttgcc tgtaacacca tttttcattg cccgtaatgg aacgcggtga gagaggcgtt
     4321 ccagttgcga accaatacgc cagttatcat taaaatcata ccagccagac aggcgcgcgc
     4381 cgggtttatg ctcatgattg aaaaagcgtt cagcgtactc cgcctcgagc cagatattac
     4441 gtgaccgcca ctcaacacct gccagccagt cgcgaacaat cccttttcct tcgctaaatt
     4501 gtccatcggc ataaccgaat ccagcaaaac cgcgccagtt atcattcagc ggtggtgaat
     4561 aaacgatggt ggttaagtcg acatcatgtt tgccactatc tggaccttcg gcatcaattc
     4621 ctgttgagcc agcgatacga agctctgcaa gattatgtac atcaaccgca cgttttaatc
     4681 gtacaacgcc gggatcttgc ggttcacgtt cgacaacatc gtgggttaag acagctgcct
     4741 gctgccattc ttgtaacgtt aatgctgtcc aggcttgttc aacctccaga ttaatattac
     4801 gtggctcgat cacttctgct ttttttaatt cattttctgc cgcacgaggc caaccgcggg
     4861 cttgtaacac actcgcgtaa tcgatgcgca gtccctgatt tcctggtgcg ttataagcaa
     4921 gttctctggt tgttatttca gcttgaggaa gatcattact atattttgct acggttgaga
     4981 gaaacgaatg cccctgtaac caggtatcat tcgggatgct cgtaggcgtg cccattagcc
     5041 gaaggaaagg cggcgaagta ttaatggtat gtttggtgac agttagcgcg ccgggataat
     5101 tttcactctc caggtggctg taaaagagat ccgcaagttc ttcatcggat aaatccgtgg
     5161 caatggtttc cttgttataa aagagctcgg tcattattga ctgtgctttt ttcggctgct
     5221 gatctttgag ataagccgac gcaacccaat atagcgccca gggcggaata atttgccccg
     5281 tcttttttaa acgctgatag tgagaaataa cgtctctata acgatcgcga gttaataacg
     5341 cgccaagatg atcaacctga atacgctggt actgggcagt gcggtctggg ttatcgtgcc
     5401 acagaatttc taatgcagcg tattgggcga gggcgcgatc ggcaatggca taacgttcat
     5461 tttcactgcg cgtaggcata aacgacagtc tgaccagttc ggcatgaata tcagcgcgaa
     5521 tatctggcgt taaattggca tcgtcaatcg cggcagctag ttgattatta cgtaatgcca
     5581 gcacgtattc tgtgggatat tgttgcttag atgcattttc aggtaatgac tctgtcatcg
     5641 cccgtaattc atcctgataa cgccccgtca gtttatagat ataggcttct gcgagtaaat
     5701 tggctttgtc cggtgctcca gatttaagct gcttaagttt aaacagcgca gtatcatagt
     5761 gacccgcatc tgccagggtt aaaatttgtc cccgttgata atccttattt tgcggctcca
     5821 gagagagcgc cttttgccac agtgtaagcg agttttgcca ttgttgcagg ttacgataag
     5881 cgacggcgac agctgcataa ccacgcgctg gtaattgctg atgacgatag cggttgtaaa
     5941 cggtaataac ctgtttatct tgcccggccc ataaggcaat ctgtaaccag tcagcaattt
     6001 ggttattact gagtgttgat ttttgtgcaa accatgacaa agctggctga gtatttcctt
     6061 tgcgagcttc aataatcaat gcatcatagg cgttattaac agcactcttc gccgcgggac
     6121 tcgctgctaa aaatgcagca gtaagaattt tcaaagccca tttggttttc gggcaccttt
     6181 ttctgctact tgaatacatc ctgtattact ccatgtattg ccaaaatctc tctctgtatc
     6241 taattacagg taactgaaaa gaaagatatt tttgcatctc ataatccgtt attaaacgcg
     6301 gaagagagac gtgaattgtt gatgatgaga agaagaaatg atgagcagag tgtccatata
     6361 aaatcctttt ctcgcccgaa aatccattcc aatgatgagg atcttcagga atacggcata
     6421 aatcccaata cctttttcaa aataaattag gattaaaata attaaatcag taaattccga
     6481 tgcatgattt cggatttttc gaaagtcctg atgaaaggca gcgctactgc actcacggat
     6541 ttaattgtta ttcctaatgt atctattaat taatgcttta taaggatttt attataatct
     6601 tttataagta tttgcgcctg ccttgcagca aaaaatgtat aaagcattaa agtgataata
     6661 attgcattct gcaactatca tcatcggtat tgtttgtggg tggattttca tattgacaac
     6721 agtacagttc ttatatctat taataataaa aagggatcta caacctacag gttggtgtag
     6781 ctttatggaa aaagactatt tgggaattag tagtactgta ttagtgagct tattgtttgg
     6841 gcttgcattg gttttggtta atagttggtt caatcagcca accgttgagg aagttgttcc
     6901 acgatcaacc tatctgatgg ttatgattgc tttatttttt atcgatactg ttgcatttat
     6961 ttttatgcag ttgtatttca tttatgaccg taggcaattt tcaaactgta tacttagttt
     7021 ggcttttcta agttgtttga tttactttgt taaaaccgtc attatcattc agcaaattat
     7081 tgaggggcgt ttgacaagca gtgttgttca aaatgacatt gcaatttatt atttgtttcg
     7141 tcagatgagt ttgtgcatgt taatatttct ggcattgata aataaagtta gtgaaaacac
     7201 aaaacagcgc aatttatttt ccaaaaaagt gactttgtgc ataagtttgt tttttgttct
     7261 tggggggccg atagttgctc acatattttc tagccattat gagagctacg atttacacat
     7321 tgccgagtta accaatgaaa atggtcaggt ggtttggaaa gcctcatatg ttaccataat
     7381 gattttcatg tggttaacct tgctttcggt taatctatac tttaatggtt tacgctgtga
     7441 tatctggaac ggggtgtcgg tgattgcttt ttgtgccgtg ctgtacaata tctctctgtt
     7501 atttatgagc aggtatagcg tgtccatttg gtatattagt cgcactattg aagtagtcag
     7561 taagttaacc gttttggtaa tttttatgtg ccatatattc agtgcgctac gagtaacaaa
     7621 ggacattgca catcgcgatc ccttaaccaa tatattcaac agaaattatt tttttaatga
     7681 actgacggtt caatcagcat cggccaaaaa aacaccttat tgcgtcatga ttatggatat
     7741 tgaccacttc aaaaaagtca acgacacctg gggacatccg gttggcgatc aagtgataaa
     7801 gacagtggtg aatatcattg ggaaaagcat acgaccagat gaccttttag cgcgcgttgg
     7861 cggcgaagag tttggcgtct tgctgacgga gatcgatact gaatgtgcga aagctttagc
     7921 ggaaaggatt cgggaaaatg ttgaacgttt aactggcgat aatcctgaat acgccatacc
     7981 acaaaaagtg acgattagta ttggcgctgt tgtaacgcag aaaaatgagt taaaccctaa
     8041 ggagatctat caactggctg ataacgcact ttacgaggcg aaagagaccg ggcgtaataa
     8101 ggtggtagta aaagaggcag aaattcttat caaccgcaag gatgatgaat gagtctgtcg
     8161 ctagttgtca acctccgcct ggggcttctc atccccc
//
LOCUS       DAOFSV010000176         8175 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.176, whole genome shotgun sequence.
ACCESSION   DAOFSV010000176 DAOFSV010000000
VERSION     DAOFSV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.176"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..200)
                     /locus_tag="QBH27_004190"
     CDS             complement(<1..200)
                     /locus_tag="QBH27_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ7016435.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTW"
     gene            413..1204
                     /locus_tag="QBH27_004191"
     CDS             413..1204
                     /locus_tag="QBH27_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDQ7016436.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1214..2056
                     /locus_tag="QBH27_004192"
     CDS             1214..2056
                     /locus_tag="QBH27_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDQ7016437.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQTARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2066..2842
                     /locus_tag="QBH27_004193"
     CDS             2066..2842
                     /locus_tag="QBH27_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDQ7016438.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            2852..4393
                     /locus_tag="QBH27_004194"
     CDS             2852..4393
                     /locus_tag="QBH27_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDQ7016439.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKNNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFM
                     PMEDA"
     gene            4390..6462
                     /locus_tag="QBH27_004195"
     CDS             4390..6462
                     /locus_tag="QBH27_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDQ7016440.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRTYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            6555..7832
                     /locus_tag="QBH27_004196"
     CDS             6555..7832
                     /locus_tag="QBH27_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016441.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATS"
     gene            complement(7975..>8175)
                     /locus_tag="QBH27_004197"
     CDS             complement(7975..>8175)
                     /locus_tag="QBH27_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HipA"
                     /protein_id="HDQ7016442.1"
                     /translation="ITENDNALDLELAREVAEYFRLSLTEADDIIANFRGIVSQWRII
                     AERLRLPGREQELMAEAFRGAI"
BASE COUNT         2023 a         1979 c         2097 g         2076 t
ORIGIN      
        1 ttccaggtat attccagtac ttcgataaat aaattttctt tattaccaaa gtggtgataa
       61 accgtctgtt tactgcaccc cgcggccttg acgatattat cgactctggc cccttcgtaa
      121 ccatattcgg caaattcatt aaccgcgcat aacagcagct tttctttcag gctctgtacg
      181 ttgctggtat caagatacat aacgcttccc cacgacctct tcgtggtgac tatcataacc
      241 tgatttgatt tcagcttcag aaaaaagggc aaaaacttgt actttcatca ggttatcaaa
      301 tgtcctgtga tcgccatcgc atatcaacca actagatggt tgatcttttt atcctccagt
      361 cgcaagctaa tcatcactaa ggaaacaaaa ttcaaaaaaa gagagctatt gtatgaaatc
      421 cactcgtcct gttgccgtga ttaccggagc cgctcgtggt attggaaagg gctgtgcgct
      481 ggagcttgcg cgtggcggat ttaatctgct gattaatgat cgtccggatg cggatagcgt
      541 agaaaaatta catgccacac aacaagaatg tctggcggaa ggcgtggaag tgatttgctt
      601 ccctgctgat gtgggcgatc tctccctgca tgaagagatg ctcgacgcag cgcaaaacca
      661 gtggggacgt ctggactgcc tgctcaacaa cgcaggcatt tcagtgaaaa aacgcggtga
      721 ccttctcgac ctcgaaccag acagcttcga ccaaaatatt gccattaaca cccgcgcacc
      781 gttcttcctt gctcaggcat tcagtaagcg cttactggca cagccgaaac cggaagcaga
      841 actgccgcac cgctcgatta tttttgtcag ttccatcaac gccatcatgc tggcgatgaa
      901 tcgcggcgaa tataccattg ccaaaactgc cgtctctgcc gctgctcgtc tgtttgctgc
      961 tcgcctttgt aatgaacaaa ttggcgtata tgaagtgcgt cctggtctca tcaaaaccga
     1021 tatgactatt ccggcaacgg cctattacga cgaactgatt gccaaaggtc tggtgccgtg
     1081 gggacgttgg ggttatccgg cggatattgc ttccactgtc cgcgcgatgg cggaaggtaa
     1141 acttatttac acctgtggtc aggcggtcgc catcgacggc ggcttaagta tgccgcgctt
     1201 ctgaggctga atcatgagag atctgttaca acgcccggat ttattcagta tcaataccgc
     1261 gacactgggc tataaaacgc cgctacccgc cattattgat gcctgcgccg cacgcggtat
     1321 tggcgcgatt gccccctggc gcagggagtt gcagagtgaa gatctccagc aaaccgcccg
     1381 ccagcttgct gccagtaaca tgaacgtttc cggtttgtgc cgtagcacct actacaccgc
     1441 accgacgctg gcggagcgca aactggcgat tgacgataac cgccgggcgc tggacgacgc
     1501 cgctgtactg aacgccgcct gttatatgca ggtggttggc ggtctgccaa tgggaacaaa
     1561 agatctgtac gaagcacggg aacaggtgaa acaagggatt cgccagttgc tgccacattc
     1621 gaaagaggtc ggcgtgccaa ttgcactgga gccactgcac ccgatgactg ctgccaatcg
     1681 ttcttgtctg tgcacgttac gtcaggcgct ggactggtgt gatgaactcg atcccgatgg
     1741 cgaatttggc ctcggcgtgg cggtagatgt ctaccatgtc tggtgggacc cggatctcgc
     1801 cagtcagatc ctgcgtgccg gaaaacgcat cctcgcgttt catgtttccg actggttagt
     1861 gccgaccacc gatctggtca atgaccgtgg aatgccggga gatggtgtca tcaatattcc
     1921 gtcaattcgt cggctggttg aaaacgccgg atttaatggc gctattgaac tggaaatatt
     1981 ctcaccgtac tggtggcaga aagatattaa cagcacgctg gatattagcg tcgatcgcat
     2041 cgcgcattat tgttaaggaa tgatcatgaa tgaccaaccc gttctgttca gcagagcggc
     2101 ggcttcttgc cgcctgacat tgaaccgtga agataaatgt catgctatta atgaagaaat
     2161 gatcgaatct ctcgaccatt atttaaatga aattgaaaat gacacggcat tacgcttagt
     2221 cgaattaacg gcaaccggcg ataaattctt ttgtgcgggt ggcgatatta aatcgtggtc
     2281 ggcctattcg ccattagata tgggcagaaa atggattaaa cgcggtaatg acgtttttaa
     2341 tcgcctgcga aacttacccc aattaaccgt tgcgaatctc aacggtcata ccatcggcgg
     2401 tggtattgaa cttgccttgt gctgcgatat tcgtatcgct cgccccggcg cgaaattctc
     2461 taacccggaa gtgatgctcg gcatggtgcc aggctggatg ggcattgagc gagtgcttaa
     2521 tcaggttggt cctgtggtcg gtcgccagat gctgatgctg ggtaaacgcc tgacggcgca
     2581 ggaagctcag acagcgaatt taattgatga agtggtagaa aaagaacagg tggaaagctg
     2641 gatggcgaac cagttagcgc agctggaaaa atgtggtccg gtggcactgg cacatattaa
     2701 gcaactgatt ctggcgctgg aaaataaaca cgccgattac tcgcaccagt tgctggctgg
     2761 attaatgtcc gccactcagg attgccaaca ggcgacgcac gcatttgccg aaaaagccgg
     2821 cgtgagcttc cacaatcaat aaggaaattg tatgcgtaag ttatcaaccg ccgagacact
     2881 ggcggcgcaa attcaggacg gcgcaaccat tgccattagc ggcaacggcg gcggcatggt
     2941 ggaagctgac catattctgg ccgccattga agcgcgcttt ctgcaaaccg gacacccgcg
     3001 cgatctgaca ttaatccact cgctgggtat tggcgatcgc gacagcaaag gcactaaccg
     3061 ttttgctcac gccgaaatgc tcaaacgcat tattgccgga cattttacct ggtcgcccaa
     3121 gatgcaggaa ctggtgaaaa acaatgctat tgaagcctac tgtttccctg gtggcgtaat
     3181 tcaggcactg ctgcgggaga tcggtgccgg acgtccgggg ctttttaccc acgttgggct
     3241 gggatcgttt gttgatccac gcaatggtgg cggtaagtcg aatgaatgca ctaccgacga
     3301 cctggtagaa ctgattgaaa tcgacggtga gaccaaactt cgttatcgcc ctttcaaagt
     3361 ggattacgcg attttgcgtg gcacttatgc cgatccacga ggcaacgtta gcctcgaaga
     3421 agaagcgatt gatatggata gctattcgat ggcgctggca gcacacaaca gcggcggcaa
     3481 agtgttcgta caggtacgcg atgtgctgga agcaggtacc attgaaccac gtcgggtcaa
     3541 gctaccagga attctggttg atggcatcgt tgagcaccgc gaacaaccgc aaacctatct
     3601 tggtggttac gacctgacca tcagcggtca acatcgccgt ctaagttcta acgacgctat
     3661 tgaactggtt agtcatccgg tgcgtcgcct gattgcccgt cgggcagcgc gggaactggt
     3721 ggcaggcgct tcaaccaact ttggctttgg tattcccggc ggtattcctg gggttgcgct
     3781 gcgcgaaggc gtgccttatc aaagtttgtg gctgagtgta gaacagggtg tgcataacgg
     3841 catgatgctt gatgatgctc tgttcggctg cgcccgtaac gccgatgcca ttattccatc
     3901 actcgatcaa tttgaattct acagtggcgg cggcatcgat atcaccttcc tcggcatggg
     3961 agagatggat caatacggta acgtcaacgt atcccatctc aatggcaatc tgattggccc
     4021 cggcggattt ctcgaaattg cacaaaacgc ccgtaaagtg gtgttttgcg gtacgttcga
     4081 cgccaaaggc agcaagattg atgtaacgcc agatggcttg catatcgccc agtcaggtca
     4141 aatccctaaa ctggttaccc aggtggaaaa aattactttt agcgccgcct acgcacagca
     4201 aagtggtcag gaagtgttgt atatcactga acgcgcagta ttccagttaa cggcagaagg
     4261 cgttgaatta attgaaatcg cgccgggtgt ggagattgag cgcgacattc tgccgtatat
     4321 ggccttccgt ccgattatca ggcacccacg tctgatggaa agtagcctgt ttatgccaat
     4381 ggaggatgca tgagtcagct tgatgacacc atccttgatg cactgacgca cgttactttc
     4441 ccaaagggtt ttgcacaggc agaacccgca tgggttgtca cggttgacgg cgttgattac
     4501 ccactctgga aaacagatgc cctggtagtc ggcagtggtg cagccgggct acgtgcagct
     4561 gtggaactta aacgccgcca gcaaaatgtg ttaatcgcca ccgccgggtt atatatgggg
     4621 acgtcggcat gttctggctc tgataaacaa acactgttta ccgccgctac tgcgggcaac
     4681 ggcgacaact tcaccaaact ggcagaggca ctggcgagcg gtggggcgat ggatcacgac
     4741 accgcttatg ttgaagcggt aggttctctg cacactcttg gcgggttgca atatcttggt
     4801 ctggaattac cggaagatcg ctatggcgcg attcttcgtt atcaaaccga ccatgacgaa
     4861 gccgggcgtg caacctcgtg tgggccgcgt acctcaaggt taatggtgaa agtgctgctg
     4921 gaagaagtac agcgtcttgc tatcccaatg ttgaccagtg caacagtgat taaactgctg
     4981 catcagcgtg acgaaaacgg tgaagaccgt gtggcggggg caatcctcgc gaccggtcat
     5041 cgcgcccata acccatgggg gctggcaatt gtgactgcgc ccaatgtggt actggcaaca
     5101 ggagggcctg gcgagcttta tcgcgacagt gtttatccac acaaatgttt cggctcgctg
     5161 ggtctggcgc tggaggaagg tctgacgcta accaatctga ctgaaagcca gtttggtatc
     5221 ggcacaccgc gcagcacgtt tccgtggaat ttgtccggca cctatgtgca ggtgatcccg
     5281 tatatctatt ccgtagatgc tgagggtaac gagtataact tcctcgcgga ttactatcgc
     5341 accacccagg agctagcttc aaacatcttc cgtaaaggct atcagtggcc gttccacgcc
     5401 acgcgggtaa tggattttgg ctccagcttg ttagatatgg cagtagcgca agagcagcaa
     5461 tcagggcgtc aggtatttat ggatttcaat cgcaatcctg aagcggtgcc gggtgacctg
     5521 cccttctcat tagatcgact ggacgacgac gttcgcacgt atctggaaaa taacgacgct
     5581 ctggcaccat cgcccatcga acgcctgcaa cgaatgaatc cgctgtctat ctcgctgtat
     5641 aagatgcacg gttacgatct caccacgcag ccattgcagt ttgccatgaa taatcagcac
     5701 atgaatggcg gcattgaagt cgatatctgg ggacaaacat ctctgcccgg ttgttttgcg
     5761 gtgggggaag ttgctggcac acacggcgtc acccgacctg gcggcgcggc attgaatgcc
     5821 gggcaggttt ttgctgttcg tctggcacgt tttattggtt gcacgcaaaa gcggaatatt
     5881 gatggagata tagcacagct ggcagctccg gcactggctt ctataagaga gatcattact
     5941 caggcgcacg ataacgggac cgggatgccg ttgtcggttg tgagagaaaa aattcaggca
     6001 cgaatgtctg accatgcggg atttatttgc catgccgata aagtccgacg cgccactcgt
     6061 gatgccctgc tattgaacga atttgtccaa cgacatggat tggcaatcaa acatgtgggt
     6121 gaagttgccg agctgtttat gtggcggcat atgacgctga cttctgccgc ggtattaaca
     6181 caattaacgc attatattga tgctggtggt ggtagccgtg gggcgcgtat ggttattgat
     6241 ccacaaggca aatgcctacc acaaactcgt cgcggcgcaa aagaagaatg gcgttttcgc
     6301 tctgaactcg ctgaagacaa aaatcacaaa ttaacaattc aatattcgca aggttctttt
     6361 attaccgaag taaagtcttt acgtatgcaa ccgtgtatta acggtattta ctttgaaaaa
     6421 aactggccag actttttaaa gggagacatt tacacacaat aatttccaat atttcctctg
     6481 tacctgattt ttattgccta ttacatggaa ataccaacaa ccagggttta ttaatataat
     6541 taaaaggttt aaaaatgaat aaaataaaga attatcgatg gcatatgatt gccctcgtat
     6601 gctttatcac tgtaatcaat tatctggaca gaacggcgtt aggtattgcg gctccaacga
     6661 ttatggagac aaccggaata accaaagaac aatattcatg gattgtcagt gcattccagt
     6721 tggcctatac attggggcaa ccgataatgg gcttctttat tgataccgtg ggtctgaagt
     6781 taagttttgc gatatgtgcc gcaatctggg gcctggcgac aatggggcat gccttgacag
     6841 gaacatggtc aggtctggca tttatgcgcg ccctgatggg tttcagcgaa gcatcggcaa
     6901 ttcctgcggg tgtaaaaacc gcatcaacat ggttcccggc aaaagagcgt ggcgtggcga
     6961 caggtgtttt caatatgggc acctcactcg gcgcgatgct tgctccaccg ttgattgcct
     7021 ggtgcattat gtttcatagc tggcaatttg cgtttattgt ctcaggtagc cttgctttac
     7081 tcgcggcttt attttggttc ttttgttata aagatccaaa agatgctaaa cgcctttctg
     7141 atgaagagcg ccactatatt gagtctgggc aagaacagca tcttaaaaca gataagaaag
     7201 aaaaaacgtc aatcaagcat atcctcagcc aacgtaattt ctgggggatt ggcatcgcgc
     7261 gttttctcgc tgacccggca tggggaacca ttaacttctg ggtaccgatt ttcttcgtcg
     7321 aaacgctgca ttttagcctg aaagaaattg ccatgttcgt ctggctacct ttcctgctgg
     7381 gcgatctcgg ctgtttagcc agtggttttg tcgcgaagtt cttccacgat cgcggcgtga
     7441 gtttaattaa ctcacgaaga attaccttca ctattgcagc cgtcattatg atgacgattg
     7501 gtctggtgag tattgtcgaa aatccctaca tcgccgtatt actgattagc attggcgcat
     7561 tctcgcatca atgtctttct actgttgcag caactctggg cggtgaccta ttcaaaaaag
     7621 acgaagtagc taccgctgtg ggtatggcag gagcctgtgc atggagcggt cagttgattt
     7681 tcaacctgtt tatcggggca ttcgttcaca ttatcggctt cgcgccgttc tttattgccc
     7741 tggctttctt tgacattatt ggcgccattg cgctgtggac gcttatcaaa gttaaagacg
     7801 aagaaccaca agtacagtta gcgacaagct aattatttta catagaaacg ctataattta
     7861 gctttgtagc gtttctattt ttctcataaa ttaaacgtct cttccaaaac gttcagtatc
     7921 tactggcatt gcttcgcaaa tttatgattt agcgaatata tcgaaataac tattctaaat
     7981 tgcccccctg aacgcctctg ccattaactc ttgctcacga ccaggtaatc gaagacgctc
     8041 ggcaatgatt cgccactgac tgacaatccc tctaaaatta gcaataatgt catcagcttc
     8101 cgtcaaacta agtcgaaaat actctgcaac ttctctagct aattccaaat caagtgcatt
     8161 gtcattttcc gtaat
//
LOCUS       DAOFSV010000177         8138 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.177, whole genome shotgun sequence.
ACCESSION   DAOFSV010000177 DAOFSV010000000
VERSION     DAOFSV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.177"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            208..858
                     /gene="zinT"
                     /locus_tag="QBH27_004198"
     CDS             208..858
                     /gene="zinT"
                     /locus_tag="QBH27_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDQ7016443.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1528..1617)
                     /locus_tag="QBH27_004199"
     tRNA            complement(1528..1617)
                     /locus_tag="QBH27_004199"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1581..1583),aa:Ser,seq:cga)
     gene            1711..2508
                     /gene="mtfA"
                     /locus_tag="QBH27_004200"
     CDS             1711..2508
                     /gene="mtfA"
                     /locus_tag="QBH27_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDQ7016444.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2609..2684
                     /locus_tag="QBH27_004201"
     tRNA            2609..2684
                     /locus_tag="QBH27_004201"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2642..2644,aa:Asn,seq:gtt)
     gene            2846..4108
                     /locus_tag="QBH27_004202"
     CDS             2846..4108
                     /locus_tag="QBH27_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ7016445.1"
                     /translation="MSLTSAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4302..5606)
                     /gene="ybtS"
                     /locus_tag="QBH27_004203"
     CDS             complement(4302..5606)
                     /gene="ybtS"
                     /locus_tag="QBH27_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDQ7016446.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5634..6914)
                     /gene="ybtX"
                     /locus_tag="QBH27_004204"
     CDS             complement(5634..6914)
                     /gene="ybtX"
                     /locus_tag="QBH27_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDQ7016447.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6907..>8138)
                     /gene="ybtQ"
                     /locus_tag="QBH27_004205"
     CDS             complement(6907..>8138)
                     /gene="ybtQ"
                     /locus_tag="QBH27_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDQ7016448.1"
                     /translation="MRRQMQTLGEAHQRLSGDIVEFAQGMMVLRTCGSDADKSRALLA
                     HFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVWVVTGTLNLAFLIAAVAM
                     IMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAEQSEMPERYDIRFDNVSY
                     RYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLMRYADPQQGQISIGGVDI
                     RRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQEVEEAARAAQCLEFISRL
                     PQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEPTAALDIESELAVQKAID
                     NLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQLLSHHGRYQALWQAQMAA
                     RVWRDDGVSASGEWGHE"
BASE COUNT         2084 a         2205 c         1939 g         1910 t
ORIGIN      
        1 cgccctatcc ggcccacaca gatcgcatca agttatgttt cgtttgtcat caacctttca
       61 tcaccagaat aatttatttt atttcaataa gatagttcaa tacacctgag aataccatgc
      121 tctcatttcc taatgagttg ttgcattttt tcatatgtta caatataaca ttacacatca
      181 tgtacattaa ctctggagga aattgttttg gcgattcgtc ttcataaact ggctgttgct
      241 ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg gtcatcactc acacggcaaa
      301 cccttaacag aggtcgaaca aaaagctgct aatggtgttt ttgatgatac caatgtacaa
      361 aaccgaacgc tcagtgactg ggatggagtc tggcaatccg tttacccttt actgcaaagt
      421 ggcaaacttg accccgtctt tcagaagaaa gcggatgcag ataaaaccaa aacatttgct
      481 gaaattaaag attattatcg caaaggttat gcgacagata tcgaaatgat tggcattgag
      541 gacggcattg tcgaattcca tagaaataat gaaacaacat cctgtaaata tgattacgat
      601 ggatacaaaa tactcaccta taaatcaggc aagaaaggcg ttcgttactt attcgaatgt
      661 aaagatcctg aaagcaaagc ccctaaatat atacaattta gcgatcatat aattgcacca
      721 cgaaaatcat ctcattttca catttttatg ggtaatgatt cccagcaatc tttattgaat
      781 gaaatggaaa actggccaac gtattatccc tatcagttga gtagcgaaga agtggtcgag
      841 gagatgatgt ctcattgatt tttttgcgct gacttcagtt atttaacaga acagtgcctg
      901 gtagtaacaa caataattta ccaagacaaa taagtaagac aaactcagac acgctcatac
      961 ttgagtcgtg tcttttttat tatgtctgta gaaaaaaact gagaaaaagg cgaacaataa
     1021 atacacaact cagccataca gactggcagc gcaaatgtct ccaggagcaa gtcatctgtc
     1081 atggtaaagc taaccagaaa tataggttaa ttttggtgtt taaacctttt ttacttgtta
     1141 gtatgataca aaggctttca aaaaagctgg gaaattcaac aactcttgga aattatggac
     1201 tttgtcccgc ggctttaatc tgacggccgc gttccttttt tggctcaatt attgacccct
     1261 tctctacgca acttcagttc ccgccagcaa ctttgcggca gtttcatagg atcaatgtct
     1321 aaaagaataa tggtgaccga taagaaaacg actgaataac tgtagatttt cgctcgaaac
     1381 cttcctgtca gacccatagc gaatcaagtg ctgaatgtca cagtatcgaa cagaaaacag
     1441 tgacgatcta acccttcaag aatatcctac gattgttctg tttaggaaaa gcaaggcggg
     1501 aagtcgggag ataagtcatt gataaagtgg cggagagagg gggatttgaa cccccggtag
     1561 agttgcccct actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt
     1621 cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtc cgtgcaattt
     1681 tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa
     1741 tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct tttaacgtgt
     1801 ctgacagaac aggaacaaag caaattggtc gctcttgccg aacgtttttt acagcaaaaa
     1861 cggcttgttc ctttacaggg ctttgagctg aattcattaa gaagctgccg gatagcactt
     1921 ctattttgcc tgcccgttct ggagttagga ctggaatggc tggatggttt tcatgaaatc
     1981 ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg
     2041 cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac
     2101 tggttggata tacaagattc ttttgatgct tctggtttta acctgattat tcatgaagtc
     2161 gctcataagc tggacacccg taacggcgat cgcgccagcg gagttccctt tatttcgttg
     2221 cgtgaggttg ctggctggga acacgatctt catgctgcaa tgaacaacat tcaggaagaa
     2281 atcgaattag ttggtgagaa tgcggcgagc attgatgctt atgctgccag tgatcctgct
     2341 gaatgttttg ccgtactttc tgaatatttc tttagcgccc cagaactttt tgctcctcgt
     2401 ttcccttcat tgtggcaacg tttctgtcaa ttttatcaac aagatccttt gcagagactg
     2461 catcacgcta atgatacaga ctcgttttcg gcgacgaatg ttcattaatt aacaactttg
     2521 cagattaatt aaccaattga aatggcttat gaaatttagt gttgacagac aaggtaccgc
     2581 taagtaatat gcgccccgtt ctcacgattc ctctgtagtt cagtcggtag aacggcggac
     2641 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaattttc tgttttcatg
     2701 catccttgcg aatctttatc tgatgttgat tcaacaggtt agtgtgaaaa ctcttctcag
     2761 tgcattttga ttttacccta cgcatcggga aaaattggtg gtcaaatctg gggtcaggtt
     2821 agttcgataa tggagtgacc cccatatgtc ccttaccagc gcaaaaatcc gcaccctcaa
     2881 gccttctgat aaacccttta aagtctccga ttctcacggt ctgtatctgc gggtcaagcc
     2941 gggtggctcc cgccactggt atctcaaata ccgtattagc ggtaaagaat cccgcattgc
     3001 gctgggtacc tatccagcca tctccctgtc tgatgcgcga cagcaacgtg aaggtatccg
     3061 taaaatgctg gcgctgaata tcaacccggt acagcagcgg gctgctgtac gtggctcacg
     3121 aacaccggag aaagttttta aaaacgtggc gctggcgtgg cataaaagta acaggaaatg
     3181 gtcgcagaac accgccgacc gtctgcttgc cagcctgaac aatcacatct ttccggtcat
     3241 cgggaaccta cctgtatcag aacttaaacc ccgtcatttc attgacctgc tgaaagggat
     3301 cgaggaaaaa ggtctgctgg aggttgcgtc ccgcacacgg cagcacctga gtaacataat
     3361 gcgccatgcg gtccatcagg agttaatcga tacgaaccct gcagcaaacc ttggcggcgt
     3421 gaccacacct cctgtcagac ggcactatcc tgccctgccg ctggagcggc tgcctgaact
     3481 gcttgaacgt attggggcat atcatcaggg ccgtgaactg acccggcatg ccgttctgct
     3541 gatgctgcat gtgttcattc gctccagtga actgcgtttc gcccgctggt cagagattga
     3601 tttcacaaac cgagtctgga cgatacccgc gacgcgagaa cccattattg gcgtgcgtta
     3661 ttccggccgc ggggcaaaaa tgcgaatgcc gcatatcgtc cccctctcag aacagtccat
     3721 cgccattctg aaacagatta aggatatcac cggtaataat gaactgatct tccccggcga
     3781 ccataacccg tataagccaa tgtgtgaaaa cacggtcaat aaggcactgc gggtgatggg
     3841 ttacgacacg aaaaaggata tctgcggtca cggcttccgg gcaatggcat gcagtgcgct
     3901 gatggaatcg ggtttatggg caaaggacgc agtagaacgc cagatgagtc atcaggagcg
     3961 caataccgtg cgcatggctt atattcataa ggcagagcac ctggaagccc gcaaagcgat
     4021 gatgcagtgg tggtcggatt atctggaagc atgccgagaa tcttatgcac cgccttatac
     4081 aattggtaaa aataagttta tcccatagta tactaacgaa tcccacactc tggcaaatta
     4141 ccaccacctc cacaagaggt ggttctatat agacttttat caacttcatg acgaaaaaca
     4201 cctattttat cccaaaagaa gacaatagag tcacagagga atcaaagagc gactcaattg
     4261 tctttggaca gtctggttgt gaggttagaa aacagttact cctacaccat taaatagggc
     4321 gcaatgctcg ctaatttctc ccgggtttct gtcagttcgc gttccggcgt agattgcgcg
     4381 atgattcccg cccccgcctg tatccagcag cgctggctat cctgaaatac ggaacgcaga
     4441 actagcgccg catcaaagcg cgtatcgtcc agcagcagga ttgcgccgga ataaagctct
     4501 cgcggcgtct tctcaatttg cataatcgcg ttcagagcag catttttagg gatacctgag
     4561 gcggtaatcg acggaaacag cacggtaaac gcatcccagg catccttgtt ttccgcaagc
     4621 tgaccgctca cgccagaccc cagatgctga acgctgccgc gctggcgaac cgacattaaa
     4681 tcttcaacca ccacgctgcc cggctggcaa acggcctcca gttcagcaat agcttctttg
     4741 acagaaagga tatgctcaag cacctcttta ctgtcgtgca gcagttctgc ctctttcgcc
     4801 ttattatgct ccgggtttcc catgcgatcg cgggtgcccg caagcggttc agtgaccact
     4861 ttattgcccg tcactgacat caccagttct gggctaaagc ccagcgcttc gcgtccttcc
     4921 tgacggaaca taaacgagcg cacaggcgtg tttgcctgcc gcccgtataa cagcgtggcg
     4981 ggcatatcaa tccgcgatgg caggggaatg gcgcgcgaga caatcacttt gacatactcg
     5041 ccacggcgga tctccgcaac ggcgcgcgca acctgttgtt tatatgcctc accattgagg
     5101 gcggtatcca ccgccagtgg tgcgttctgc gtcgttgtac cggcctcttt tacccactcg
     5161 cacaaacgtc ggcacccgtc ggcggagtcc gcataaacgg tgacatttcc cttttcaaaa
     5221 ataagttctt cacggggaac ggttaacgtc agcagcggcc actccccggc gttaaaggca
     5281 atcccccgcg catgcgccgc aaagttgaac ccaacctgtc catatacccg acgtcctttc
     5341 acctgaggat gcgccataaa acgacgcgcg cagtcggcaa tggaatccac cgcccatttt
     5401 tgctccccgg catcatcaat aaatgtcgcc tgcgttccgt cggcattaat gtgcagccgt
     5461 gcctggcacc ctttgcctaa ataccaacag ggttgacgct catagacata acactcttct
     5521 tctgcgaact ggcgtaaaac gccagaaatc gtcggtagcc attgttcctc tggtaacgcc
     5581 agatgtagaa attcactgat tttcatccct tacctctctg tgttattcgc ggatcaatca
     5641 attgcgcttg tcatcagttc tcttgccgaa tgcagccgga tataagcaac ccacgccgcc
     5701 agcaacgtga atgccccggc aaacaggaag caggccgcat agccaaaatg ttgagcaaca
     5761 acgccaccga taacgcctgc cagaatggcg attgccgcat cagtacattg aaagagggtg
     5821 aagtcgacac cggcctgcaa aggcgatgaa agcgacatca gcgtggcgta aagacagacc
     5881 agcgcgcagg ccagcgaaat ggactggaca atgaccagac actggagaat ctgcagcagc
     5941 agatgacccg gcgccatcat gagcgtcatc accaccgcca gcagcgcgat cccctggacg
     6001 ccataagccg tcaacagcgc tctgccgggt gaggtgtatt tcatcagcaa tccgccggcc
     6061 agcgttcctg ctatgcccgc tgcaatattg ccgccgctga acagcgcgcc caatgcggac
     6121 atgctcaacc catgatccaa caacagcggt gccagaagag gcagcacaaa ccgcatgcct
     6181 gaattcagca tcaataccag cagtaagccc aggcgcgcct gcttcctcct tagcgcataa
     6241 cctaacgccg gaacatgcgg gatagtcgct gttcgcgtcg gctccgtaat gcgccacagc
     6301 gggagtgaca gcgccataat cagcaccgcc atcatcagca tggcgacagg ccagccggat
     6361 gccgccgaca gcatcatgaa caccccgccg ccgcacatca ttcccagata gctgccgccg
     6421 acctgcacgc tgtttcccca gccgtaaccc gtgcgggtca gttgatcgac acaaaagccg
     6481 tcgctggcga tatcaatggt ggaggcgacc gtgcctgcca ccataaacag aacaaataac
     6541 gcgacgattt gcgtatcggg gaatccccct tctcgcccaa accagccaat cgctgcggca
     6601 atagtcagga tcgccgctag cgcgacctga ccacgcagga ttaacatgcg ggaacggcgt
     6661 tcctggctac cgggcggaag ccgccagcgc tcgatccacg gcgcccaaat aaacttcagc
     6721 gcccagggca gcataaacag ggttgtcgca ccggcaagcg ccagcgaacc gccagcagcg
     6781 cgcaccagcg cgggtaagga ctgcatagac agcgccgata ccagactctg cgtcacatag
     6841 acgccggcaa tagcaaaaat cacccgcccg gtcgtcaacg tcagcggttt cacattcgac
     6901 tgaacatcac tcatgccccc actctccaga cgcggaaacc ccgtcgtcgc gccacacgcg
     6961 cgcggccatt tgcgcctgcc acagcgcctg ataacgtcca tgatgtgaga gcaattgcgc
     7021 atgagtaccc tgctcaacca cctgtccctc ttccatcacc agaatgtttc cggccccggc
     7081 gatggtggat aaacggtgag cgatgataat caccgtccgg ttgtaaacca ggttatcgat
     7141 cgctttttgc accgccagct cgctttcaat atccagcgcg gcagtcggtt catcgagaat
     7201 aacgaccggc gcgtttttca ataacgctct ggcaatggaa atccgctggc gctcgccgcc
     7261 cgatagctgg ccgcccatct ctcccattgg cgtcagccag ccttgcggaa gacgggaaat
     7321 aaactcaagg cactgcgccg cgcgggcggc ctcttctacc tcctgccgcg tcgcctgtgg
     7381 gcgcgcgata cggatattcg ccagcagcgt gtcatcaaac agccagacat cctggaaaac
     7441 aacagaaatc aggctattga gctgttccgg cgtcaggcgg cgaatatcga cgccgccaat
     7501 agaaatctgc ccttgctgcg gatcggcata gcgcattaac agtttggtga cggtagtttt
     7561 gcctgcgccg gaggcaccca ccagcgcgct catactggct gccgggaacg tcaaagaaac
     7621 atgattaagc gcgtggccgt caccttcttc gtagcgatag ctgacgttgt caaagcggat
     7681 atcgtaacgt tccggcatct cgctttgctc tgcgacggga agcggtgcta tcgccataaa
     7741 ctgctcaatc ctttgcaggg cgctggcgat cagttccaca accgaggtgt agctgataaa
     7801 catcgccatc ggttctgcga agcgcataat catcgcgacg gcggcaatca aaaaggcgag
     7861 gttcagagtg cccgtcacta cccagacgat cccggataac accaccacct gtaggcccaa
     7921 ctccacgacg ctggcgatca gcatcgtcgc gccagcgccc tgacggtgag tgcgggtctg
     7981 taagttttcc agcgcattga aatgcgccag cagcgcccgg cttttatcgg catcgctgcc
     8041 gcaggtgcgc aataccatca tcccctgagc aaattcaacg atatcgccgc tcagacgctg
     8101 gtgcgcttcc cccagcgtct gcatttgtcg ccgcatcg
//
LOCUS       DAOFSV010000178         8019 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.178, whole genome shotgun sequence.
ACCESSION   DAOFSV010000178 DAOFSV010000000
VERSION     DAOFSV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.178"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(75..869)
                     /locus_tag="QBH27_004206"
     CDS             complement(75..869)
                     /locus_tag="QBH27_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDQ7016449.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            1002..1304
                     /locus_tag="QBH27_004207"
     CDS             1002..1304
                     /locus_tag="QBH27_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ7016450.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            1340..1681
                     /locus_tag="QBH27_004208"
     CDS             1340..1681
                     /locus_tag="QBH27_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDQ7016451.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(1739..4243)
                     /gene="copA"
                     /locus_tag="QBH27_004209"
     CDS             complement(1739..4243)
                     /gene="copA"
                     /locus_tag="QBH27_004209"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDQ7016452.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            4505..5437
                     /gene="glsA"
                     /locus_tag="QBH27_004210"
     CDS             4505..5437
                     /gene="glsA"
                     /locus_tag="QBH27_004210"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDQ7016453.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            5440..6732
                     /locus_tag="QBH27_004211"
     CDS             5440..6732
                     /locus_tag="QBH27_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ7016454.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            6857..7264
                     /gene="cueR"
                     /locus_tag="QBH27_004212"
     CDS             6857..7264
                     /gene="cueR"
                     /locus_tag="QBH27_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDQ7016455.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(7265..8000)
                     /locus_tag="QBH27_004213"
                     /pseudo
     CDS             complement(7265..8000)
                     /locus_tag="QBH27_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1833 a         2140 c         2137 g         1909 t
ORIGIN      
        1 gcttaaagtg acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga
       61 tattggccat ttttttagcg caacatctgc ggcaaattcc cttctccata caggtgtagt
      121 gcaccgaccg cgaccacata gcgccccggc ggcatggcgc gtaatttgtc ccgccaggcg
      181 aggtttcgct gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt
      241 atatcattat tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt
      301 gcgttggtgt gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc
      361 tggagcaaca tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt
      421 tgcttagccg cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt
      481 tgcgcctgcg tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagc
      541 gacggagaaa tgcccatctc ctggctaatg tgctgcaggt tttggagttg gtcctcgcta
      601 atgcgctctt ccagggcctc gcaggcaggc aaattggcaa aaggcgtatc gctggtggaa
      661 acatccgcct cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc
      721 aggggagcca tatcgtggct acccatatga atactgccga tcagatgaaa atggcgattg
      781 ccgggaagag agatatcgat agcaggccag gtgtaatgat taccgcgcag cgcggcccaa
      841 agcgttttta cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc
      901 gcgcggggag ggatggcgca aatggagttg attgttgcac ttgtgcacag tgcttgtgtg
      961 cattacaatg attaaataat ggacaaggag atgagagagg aatggctcaa aagaaaaata
     1021 taaggagttt tcgtgatgcc tggttggctg atttttttgt ccattcaacg ccacatcgaa
     1081 agatccctgc tgaaatccat accacgttat cacgaaagtt ggacatcatt aatgctgcca
     1141 cttctcatcg ggatttacgc tcccctccgg ggaaccgata cgaagagtta tccgggaaat
     1201 tacaggaata ctcctccatt agagtgaata aacagtaccg actgattttt aaatgggtta
     1261 atggcaaggc cgaagaattg tttttagacc cacacaacta ctaaaacata gctgtcgtat
     1321 tgacgagaaa ggtaaaacta tgaaacaggc aacaagaaaa ccgacgacac ccggagatat
     1381 tcttctctat gaatatctgg aaccgctcga tttgaaaatc aatgagttag cagagttgct
     1441 gcatgttcat cgtaatagcg tcagtgcact gatcaataac aatcgtaaac tcaccactga
     1501 gatggcattc cgtctggcga aagtttttga taccacagtc gatttttggc taaacctcca
     1561 ggcggcagtt gatctttggg aagttgaaaa caacatgcgt acccaggaag aattgggacg
     1621 gattgaaaca gtggctgaat atttggcacg ccgtgaagag cgtgcaaaaa aggtcgcgta
     1681 gaataatttc cctataacgg atgcgccacc ctaaagcagc gcatccgcaa tgatgtactt
     1741 attccttcgg tttaaaccgc agcaatcggt tggcgttact cactacggtg atcgacgaga
     1801 gcgccattgc cgctccggca accaccgggt taagcagtgt tccagtgaac ggccacaaaa
     1861 taccggcggc gaccggaata ccaatgctgt tgtagataaa cgcaccgagc agattctgct
     1921 tcatgttgcg cagcgttgcg cgggaaattg caagcgcatc cgcaacaccc atcaggctat
     1981 ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc actgccgcca cccatcgcaa
     2041 taccgacatc cgcctgagcc agcgctggcg cgtcgttaat gccgtcgcct accattgcca
     2101 cctgacgtcc ttcactttgc agacgtttga tcgcttcggc tttaccgtct ggcagcacgc
     2161 cggctatcac ctcatcaatc cctgcttctt tggcgatcgc attggcggtg gtcgggttat
     2221 ccccggttag catcaccagg cgatatcccg ctttatgcag gcgttgcagc gccgccacgc
     2281 tatcactacg caacggatcg cgtacagcca gcagggctac cgctttcccg tcaaccgcca
     2341 gcagcacagg cgttgcccct tgcgatgcct gagctgaaat atctgcttca attgctttgg
     2401 tatcgacctg ttggtcattt aacagcgcct gattgcccag caataacgca tgaccttcag
     2461 cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc gttgacctgc ggtagctgca
     2521 tatcacctgc tttatcgagg atcgcccgtg ccagagggtg gctggaacct tgctccagcg
     2581 ccgccgccag acgcaatgct tgcgcttcat caatatcagc aaatgttttt actgcgacaa
     2641 cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa cactacggtg tcgagcgtac
     2701 tggcgcgttg cagcgcgtcg gcgtcccgta ccagcacgcc aaactcagcc gcccgcccga
     2761 cgcccgaaat aatcgacatc ggcgtcgcca gccccagcgc acacggacag gcaataatca
     2821 gcaccgtggt ggcaatcacc agggtataga caatctgcgg tgccggacca aagaaatacc
     2881 agattgccgc actgatcagc gcaatcacca ccactaccgg cacaaatacg gctgagattt
     2941 tatccgccag ctgaccgatt tctggcttac tgctctgggc ctggcgcacc atacgaataa
     3001 tccgcgacag cgtagtatgg ctgccaaccg cactggcgcg aaacagcacg ctaccgtcct
     3061 gcaccactgt cccggcatgg acgctatcgc cttcgccttt ttgctgcggg ataggttcgc
     3121 ccgtcagcat cgcttcatcc agccatgctt cgccctgggt aatctctcca tctaccggta
     3181 cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg cacttccgcc agtggcacgc
     3241 ttttttcacc ttcgtcagta accaggcgcg ccgtcggcgg agttaaatcg agcaatttct
     3301 ccagcgcctt tgaagaacgc tgacgtgcgc gcgcttccag catatgaccg agattaatca
     3361 gaccgataat catcgcactg gcttcatagt aaagatgccg cgcttccatc gggaaccact
     3421 gcggccacag gttgacgctc atcgaataga gccacgccac acccgttccc agcgccacca
     3481 gcgtatccat cgtcgccgca ccgttcagca ggcttttcca tgcgctgcgg taaaaatgcc
     3541 cgccggcgaa aaccattacc gccagggtta tcaggccgat aactaaccac aggctgcggt
     3601 tgtcagcggt gaccatcatg ttatcgccga tcatccccca gaccatcacc gggataccca
     3661 ccgccagcgc gacaattgcc tgccagcgga agcgcttcat cgtagcgacg gcggtttctt
     3721 gctggcgctc gcggcgttta gcgtcatctt caatcgcttc cgcgccgtag cccgcttttt
     3781 ccaccgcctg cactaaatct tgtggggagg cactgcccat caccagcgca gtacgctccg
     3841 ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg caacgcattt tgtacgcggg
     3901 tgacacagct ggcgcagctc atgccgctca gcagcaactg ctggctgtca tcgtcatcgg
     3961 cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc ttccgacggg attgatgact
     4021 ccgccagcgg tttagccttt gggtggctta cagatgcgtc ataacccgct tgtttgatgg
     4081 tttcgattag ctgttctgca ctggcagtcc cggtaacgtg cgcttcagtg atagacacat
     4141 ccgcctgttc aacatccggg cgctgttcga gactttcttt cacgcgttta acgcagtgac
     4201 cgcaggacag gccgtccagg gtcaggtcga tagtttgtga cataaaacac tcctttaaga
     4261 cagttttgac tggctgtgat aaaggttaaa ccttccagca aggggaaggt caagaaatta
     4321 ataaaccagg cgggtaaaag tccgtaaaga ttaaaaaatc ggctcgattt gcatcaggat
     4381 tagacattta tctctttgtt ttcctgtagt taagttgcgg gtgctaagtt aaatcaggat
     4441 gcatgaaaat cggcaccggg gtgaggaatt acctcccgca tctataaaaa ggagttaaca
     4501 aaagatgtta gatgcaaaca aattacagca ggcagtggat caggcttaca cccaatttca
     4561 ctcacttaac ggcggacaaa atgccgatta cattcccttt ctggcgaatg tccctggtca
     4621 actggcggca gtggctatcg tgaccagcga tggcaacgtc tatagtgcgg gtgacagtga
     4681 ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg ttagcccttg cgttagaaga
     4741 tgtcggcccg caggcggtac aggacaaagt tggcgctgac ccgaccggat tgccctttaa
     4801 ctcagttatc gccttagagt tgcatggcgg caaaccgctt tcgccactgg taaatgctgg
     4861 cgctattgcc accaccagcc tgattaacgc tgaaaatact gaacaacgct ggcagcgaat
     4921 tttacatatc caacaacaac tggctggtga gcaggtagcg ctctctgatg aagtcaacca
     4981 gtcggaacaa acaaccaact tccataaccg ggccattgcc tggctgctgt actcagccgg
     5041 atatctctat tgtgatgcaa tggaagcctg tgacgtgtac acccgtcagt gctccacgct
     5101 catcaatact attgaactgg caacgcttgg cgcgacgctg gcggcgggtg gtgtgaatcc
     5161 gctgacgcat aaacgcgttc ttcaggccga caatgtgccg tacattctgg ccgaaatgat
     5221 gatggaaggg ctgtatggtc gctccggtga ctgggcgtat cgcgttggtt taccgggcaa
     5281 aagcggtgta ggtggcggta ttctggcggt cgttcctggc gtgatgggaa ttgccgcgtt
     5341 ttcaccacca ctggacgaag aaggcaacag tgttcgcggt caaaaaatgg tggcatcggt
     5401 cgctaagcaa ctcggctata acgtgtttaa gggctgatca tgatgaacac ggaaggtaat
     5461 aacggtaaca aacccctcgg tctatggaac gtcgtttcca tcggcattgg ggcaatggtg
     5521 ggggcgggga tcttcgcgct gctggggcag gctgcgttgc taatggaagc ctcgacctgg
     5581 gtcgcctttg cttttggcgg tattgtggcg atgttttccg gttatgccta tgcgcgtctg
     5641 ggggcgagct atcccagcaa tggcggtatt atcgacttct ttcgtcgcgg attaggcaac
     5701 ggcgtttttt cgctggcgct ctcgttattg tacctgttga cgctggcggt gagtatcgcc
     5761 atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt tgcatgaagg cagtcaggag
     5821 gagcacctta ttttgctcta cgcattgggg atcattgcgg tgatgacgct tttcaactcc
     5881 ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg tcggcattaa aatgatgatc
     5941 ctgttattgc tgattattgc cggtgtctgg tcgctgcaac cggcgcatat ttccgtttct
     6001 gcgcccccca gctccggggc gttcttctcc tgtatcggga taaccttcct tgcctatgcg
     6061 ggctttggca tgatggcgaa cgcggcggat aaagtgaaag atccggaggt cataatgcca
     6121 cgggcgtttc tggtggcgat tggcgttacc acgttgcttt atatctcgct ggcgctggtt
     6181 ttgcttagcg atgtatcggc attagagtta gaaaaatatg ccgataccgc cgtagcgcag
     6241 gctgcttctc cgctcctcgg gcatgtgggg tatgtgatcg tcgtcatcgg cgctttactg
     6301 gcgacggctt cagccatcaa cgcaaacctg ttcgccgtgt ttaacatcat ggacaacatg
     6361 ggtagcgaac gcgaactgcc gaagctaatg aataaacctc tgtggcggca gagtacctgg
     6421 ggtaacatca tcgtcgtggt gttgattatg ctgatgacgg cggcgctgaa tttaggctca
     6481 ctcgccagcg ttgccagtgc cacctttttg atctgctatc tggcggtgtt tgtggtggcg
     6541 atccgcctgc gtcatgatat tcacgcctcg ttgccgattc ttatcgttgg tacgttggtt
     6601 atgttgttgg tgatcgttgg ctttatctac agtctctggt cccagggcag ccgtgcgctg
     6661 atatggatta ttggcgcact cttactcagc cttattgtgg caatggtcat gaagcgcaat
     6721 aaaaccgtat aacatctctc tgtgcgcagt acctcctgta ttattgtggt ggcggtcgat
     6781 attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa accctttaac aaagcacagg
     6841 aggcgttgcg cgaacgatga acattagcga tgtagcaaaa attaccggcc tgaccagcaa
     6901 agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca gcgaaaacgg
     6961 ttatcgcacc tacacgcagc agcatctcaa cgaactgacc ttactgcgcc aggcacgcca
     7021 ggtgggcttt aacctggaag agagcggcga actggtgaat ctgtttaacg atccgcagcg
     7081 tcacagcgcc gacgtcaaac gtcgtacgct ggagaaagta gcggagatcg aacagcatat
     7141 cgaagaactg caatccatgc gtaaccagct gctggcgctg gcgaatgcct gccccggtga
     7201 tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc atcgggcggg
     7261 gtgattaagg cttgcgaaaa tagtcgtcca ttattttcgt tagctccgga cgttccccgt
     7321 catcattgat ggcaaaatcc agaaaaaaag catctgcttg cggcgttcgg ttttgcgcca
     7381 tcaaccggaa ggtgaggggg cctgaccagg tttcggatgg gctgatgtgc ttatcagggc
     7441 cgaaactctc ccatgcgctg ggcggtaatg ttttgcacgc ggtgagtttt ggtatgtggt
     7501 attgcagctc tgttttgtgc agacattccc cgccaggaaa taccagggtt tgttgctcat
     7561 cattaaaatg ggttgccgtg ctgagcacca gtgttttgag cggtcgccct tccagcgcgg
     7621 cggcagtagc cttatggtgt ccgtcgagta gaacgcagag ataggaatca gggagatacc
     7681 aggccagacc acgggcaatt ccagttttcg agagcagaca aaaaccgggc ggctccctgt
     7741 tcatcctccg caaaaccgct gtagacaaat tccgcacagg tagggcatcc agcctcggac
     7801 cagaaacgta ttaacgggac atcctgccag cagagcgaat ggataagacc gtgcgagtgc
     7861 ggatcgtaaa tcacacattg acgcagaaat cctttcccgg acttcaccga aaaaatgacg
     7921 cccgcagcgg tatttttcag cagacgtact tcaggttttc gaaaggggga aacatggcta
     7981 ctccttagcc gaacattcaa ttaacactat gtggggggc
//
LOCUS       DAOFSV010000179         7991 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.179, whole genome shotgun sequence.
ACCESSION   DAOFSV010000179 DAOFSV010000000
VERSION     DAOFSV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.179"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1149)
                     /gene="lplT"
                     /locus_tag="QBH27_004214"
     CDS             complement(<1..1149)
                     /gene="lplT"
                     /locus_tag="QBH27_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDQ7016456.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVIANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALF"
     gene            complement(1142..3301)
                     /gene="aas"
                     /locus_tag="QBH27_004215"
     CDS             complement(1142..3301)
                     /gene="aas"
                     /locus_tag="QBH27_004215"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDQ7016457.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQLKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            3887..4918
                     /gene="galR"
                     /locus_tag="QBH27_004216"
     CDS             3887..4918
                     /gene="galR"
                     /locus_tag="QBH27_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDQ7016458.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(4925..6187)
                     /gene="lysA"
                     /locus_tag="QBH27_004217"
     CDS             complement(4925..6187)
                     /gene="lysA"
                     /locus_tag="QBH27_004217"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDQ7016459.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            6309..7244
                     /gene="lysR"
                     /locus_tag="QBH27_004218"
     CDS             6309..7244
                     /gene="lysR"
                     /locus_tag="QBH27_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDQ7016460.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(7231..7923)
                     /gene="ygeA"
                     /locus_tag="QBH27_004219"
     CDS             complement(7231..7923)
                     /gene="ygeA"
                     /locus_tag="QBH27_004219"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDQ7016461.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADDRAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
BASE COUNT         2008 a         2213 c         1962 g         1808 t
ORIGIN      
        1 aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctacca ttaccgccag
       61 cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt gtactgcaat
      121 cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac
      181 gacaaaaaaa ccccccagca cgccaatcag catcaacaaa gcataggctg gcagcagctc
      241 gtgttgcagg gagaaaatca gtaccaccac gccaatcaaa atcccggctg gcatacagcg
      301 tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcaccaacca cgataccaat
      361 cgctaccatc gcgttgagat aggtcggcgt agcgttatcg gtaataccca gcgccaccgg
      421 tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc
      481 caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat tcaggaaact
      541 gcgggtcatg ctgatgagat tccaggactg ccccggacgc gccgccgcca gtttgggaat
      601 gtagatattg gcaatgaccg cgccaccgta ggccagtgcg catgcggcca gggcgatcag
      661 gacatgccag tcagccagaa caccaccagc tacggaaccg agcaaaatcg ccgctatggt
      721 agacgcctcc attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag
      781 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccaacg tatagccgag
      841 aaacggattg ataccaaagc agatactggc tgcgcccagt agcttcaggc cgttggcaaa
      901 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgcaaaaag
      961 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
     1021 cgctttcagt aacgccagag tagcaaacag tagcgcatta tcgccaaacg cagagagaaa
     1081 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
     1141 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
     1201 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
     1261 ccggcacgcc gtgctcgcgg gcatactgtt gtaacttatc gcgcgtcagt tcgttatctg
     1321 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
     1381 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
     1441 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcatcaa
     1501 aacgcacgat atcgccagtg tcataccagc cgcgctccat ttcgccgcga atattctcgg
     1561 cggtgggcac ttccagtaca ccaggcttct ccacccgcag atagccgttc attatgttcg
     1621 gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac aggcgcgcat
     1681 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
     1741 aaacgacagg cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat
     1801 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
     1861 gacgatagaa gtcatacggg ttggcaaagc gcgcgtagtg accgaggaaa gtcgaggtac
     1921 cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg taatgcagcg
     1981 ggcttggata aaggaacact tctgcacctg tgagcagtgg cgtaaacagg cctaccgtca
     2041 gcccaaagga gtgaaacagc ggtaacgccg acataaagcg atcgttggtg gtgaagtcgg
     2101 caatcgtttt aatctgctca acgttcgcca gaatgctttt atggctatgg acgacgcctt
     2161 tcggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ttcttcttcc ggctgctgtt
     2221 ttaactgtgc cagacgcggc atcagcaaat gagcgaaaat ccacactttg tcggcagtgg
     2281 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
     2341 gatgccagag tttgccttta tcgagaaact gacgggaagt gaagatggtt ttgatttcag
     2401 ccgccgtaat agcactggtc agccctttta ccccggcggt gtagttcatc attgcgggaa
     2461 tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
     2521 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
     2581 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
     2641 tcccggctcc gaagcggtac attgcgctca gtaaagattc gtacagcgtt tcacgcgggc
     2701 gcaccgccat acgcgcctcc atcattattt gatgcagcat ttcgccagcg attttgcgac
     2761 ggtcacgggc acgcggcgca tccggcatct ccacctgcgt tggtggcaaa atatgcagag
     2821 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
     2881 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaatcccg
     2941 cgccatcgta gattttcatc agcgagccag tggtggtgat gcgtccttca gggaaaatca
     3001 ccactggtcg gccctgttcc accagacgta ccagatgttt aatcgccata ggttgcgtcg
     3061 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
     3121 ttgaggtgta aacggcaaat accggacgta cgggtaaaaa caatgccagc agtatgccat
     3181 caataaaaga gacgtgatta ggcgtgatta gaacgcgctc gcccttcagt gccttggtgt
     3241 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
     3301 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
     3361 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
     3421 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
     3481 tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
     3541 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac
     3601 ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac ccgaagttga cttcacctat
     3661 caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg
     3721 caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt
     3781 tgcgtttggc tcacattccc acaatgaaaa cacgccgccc cttgaaccaa cgggcgtttt
     3841 ccgtaacact gaaagaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa
     3901 ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc
     3961 acccaaagcc agcgaagctt cccgactggc tgtgcatagt gcaatggagt ctcttagcta
     4021 tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacga tcggtctggt
     4081 cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc
     4141 ttatcacact ggtaattttt tattgattgg caacggttac cacaacgaac aaaaagagcg
     4201 tcaggccatt gagcaactga tccgccatcg ctgtgctgcg ctggttgtcc atgccaaaat
     4261 gatcccggat gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa
     4321 ccgtatcctg cctggctttg aaaaccgttg tattgctctg gacgatcgtt acggtgcctg
     4381 gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa
     4441 ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag
     4501 tggtattccg gccaatgacc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga
     4561 acaggcaatg accgagcttt tgggacgagg cagaaatttc actgcggtag cctgttataa
     4621 cgattcaatg gcggcgggcg cgatgggcgt gctcaatgat aatggtattg atgtaccggg
     4681 tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct
     4741 gaccaccgtg cgttacccaa tcgtgacgat ggcgacgcag gctgccgaac tggctttggc
     4801 gctggcggat aatcgccctc tcccggaaat cactaatgtc tttagtccga cgctggtacg
     4861 tcgccattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc
     4921 gcagttaaag caattccagc gccagtaatt cttcaatggt ctggcgacgg cgaatcaacc
     4981 gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctgttg tagttggatg
     5041 acattgacgc gccatatgcc cctgtatcat gcagtaccag ataatcaccc gccttcactt
     5101 ccggcaaggc gcgggtttcg acatttcccc cttcctgctg ggtaaagaca tcgcccgatt
     5161 cacataacgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat
     5221 cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt
     5281 tgaacccggc atcaaccaga acaaagtggc ggctacccat ttgtttgacg ctccgcactt
     5341 gagtaattaa tacgccagcc tgcgctacca gaaagcgacc cggttcaatt tccagtttca
     5401 caggatggcc caaatggcgg gcgatttgct cacgcgcggc attccacaga ccataataat
     5461 gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag
     5521 aaatagcctg taaatcctga ccgaattcga ggacttgacg caccatagca ccacatacct
     5581 gttccagatg ggcataatcg acgccagaac caatgtgcat gtgaatgccg accagctgta
     5641 gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct
     5701 tgctgttttc gccaccggta ttggtttttt ggctatgccc gtgaccaaac cccggattaa
     5761 cacgcagcca tacccgatgc cctggcgaaa cctgccccag ttggtcgagc atatcaacag
     5821 aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa
     5881 catctgccgt aaaaacaata tcatcagggt gcgtttgcgg attgtaaccc gccgccagtg
     5941 cacgctctat ttcacctaac gagacggaat ccacttttac gccctgctca cgcattaagc
     6001 gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacatca aactgtttca
     6061 gcgccgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt
     6121 ctgcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcagtgctg aacagtgaat
     6181 gtggcataac aaactccaga taagtgcttt tttatgatta cgccacatca taaaaagaat
     6241 aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga
     6301 gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg
     6361 ccggaagcct gactgaggcg gcacacctgc tacacacctc acagccaacc gtcagccgcg
     6421 aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt tgagcgcata cgtggacgat
     6481 tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac
     6541 tggatcgcat tgtcagtgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta
     6601 ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg
     6661 cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt
     6721 ggctctcggc ccagcgtcat gatttaggac tcactgaaac gctccatacg cctgcgggaa
     6781 cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg ggccatccgc
     6841 tggcggtaaa aaaggtatta acgccggatg attttcacgg tgagaactac atcagccttt
     6901 cccgtactga cagctatcgc cagttgctgg atcaattgtt tactgagaat caggttaaac
     6961 gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg
     7021 taggcgtttc agtggttaac ccgctcaccg cactggatta tgcggcaagc ggtttagtgg
     7081 tgcggcggtt cagcattgcg gttccattca ccgtcagcct gatccgcccc ctgcaccgcc
     7141 cgtcatcagc gctggtgcag gcgtttagtg agcatttaca agcgggatta ccgaaactgg
     7201 tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc
     7261 ggcatggatc gccgcggtat caaacacagg cagaacactg cgctcttctg gcaccagtaa
     7321 accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat
     7381 cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa
     7441 aataatctga ttaattttcg cccggtcatc cgcttcagga ataagacagt tgatggaaaa
     7501 ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgtg tacccagcag
     7561 cgccacacga gtcattcctg ccccggtaat tgcacgtcca gtggcatccg ctatgtgtaa
     7621 gaaaggcaga gagcaacgag actcaatggc atccgccact ttgtgcatcg tattggtgca
     7681 taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatatc
     7741 cccggttttg tcccactcac cgcgacgctg gcactcttct atttcatgaa aatcgacgct
     7801 atgtagcagc acttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat
     7861 cagacgatag taaggaatag tggattccca gctcattcct cccagcaaac caattgtttt
     7921 cattcctctc tcctgatgag tgttaccgca acaaggctga ataaggaacg aggcaaacgc
     7981 ctcaacggcc a
//
LOCUS       DAOFSV010000180         7841 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.180, whole genome shotgun sequence.
ACCESSION   DAOFSV010000180 DAOFSV010000000
VERSION     DAOFSV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.180"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            229..1122
                     /locus_tag="QBH27_004220"
     CDS             229..1122
                     /locus_tag="QBH27_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7016462.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1153..2142)
                     /locus_tag="QBH27_004221"
     CDS             complement(1153..2142)
                     /locus_tag="QBH27_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDQ7016463.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAADKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2169..3020)
                     /locus_tag="QBH27_004222"
     CDS             complement(2169..3020)
                     /locus_tag="QBH27_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDQ7016464.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            3586..7833
                     /gene="fdeC"
                     /locus_tag="QBH27_004223"
     CDS             3586..7833
                     /gene="fdeC"
                     /locus_tag="QBH27_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDQ7016465.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTANNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETESGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWIAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         2034 a         1832 c         2092 g         1883 t
ORIGIN      
        1 tggagaaaag agtaaaaata gaaagttaat gatgtgtgtc agaatatcgg taagaagaat
       61 ggcgcaatcg tgagaatacg tttattcata ataaatctcc tgaaagttta gaattatttt
      121 cgatacagcc agattatcat tgttactaat aatgtggtta aaataatagc atctatgccc
      181 cccttcatga gtgtctgtga agaggatgct tctacaagga aatcgccaat gctcaaagaa
      241 aacttcaacg aactgcaaat ctttcttgtg gtggcaagag agcgaagttt taccaaagca
      301 gcgggcaaac tcggcgtctc tcagtctgca cttagccatg caatgaaggc tctggaggaa
      361 aggctcaata tccgcctcct gacacggact acccgcagtg tggctcccac agaagcgggg
      421 gagagaatca ttgcctgcct tgaaccgcgt attgacgagc ttgaacagga actggaatca
      481 cttattcaat tgaacggcac gccctccggg aatattcggt tatctgccgg ggaacatgcc
      541 gcgcgaagtc tggtctggcc gaagctaaaa ccttttctca gagagtatcc cgaaatcaat
      601 gtcgaactgg tggtggataa cggttttgtc gatattgttg aaggccgttt tgatgccggg
      661 atacgactgg gtgaaaatgt tgataaagat atggttgcag tcaggattgg accagatatg
      721 cggatggctg tggtgggagc tccggcgtat tttgctgcaa accctgcgcc tgaaacgcct
      781 catgagctac aaaatcatcg ctgcatcaac atgcgtttgc caactgccgg tgggctttac
      841 cactgggagt tcgagcgaga agggaaaccg cttcgcgtta aggttgatgg gcaactgacg
      901 tgtagtccat tgccagaaag aattgatgcc gcgttatcag ggtttgggat cgcttgtgtc
      961 cctgaagaca tggttcagga gtatataaag tcaggcaagc ttattcaggt gttacaggag
     1021 tggtgtccaa cttttcctgg gtattatctt tactacccca gccgcaagca gcatccgcca
     1081 gcttttgcgc tgttgatcga tgcgcttcgc tacaccgaat aacgggtcct ttatcctgga
     1141 cccgtttggg tattagcggc ctacgcgtgc ctgatgttca ggcgagtaac gttcaccgac
     1201 gattttaatt gtttccagcg cctcggttat ctgctgcgta tctttctgcg aaagaacaat
     1261 gtcagcagcg gccaggtttt cttccagccg gttcagttta gtggtgccag ggatgggaac
     1321 aatccacggc ttttgtgcta atagccatgc cagtgcgatt tgtgcagacg ttaccccttt
     1381 atctgcagcc agttcaccta acaaggtgac cagtttttca ttggcttcaa tggcttgtgc
     1441 tgcgaaacgc ggtacggtgc tgcggtaatc atctttgccg aaagtggttc ccggcttaat
     1501 cgcccctgtc aggaaacctt tacctagtgg gctgaagggg acaaaaccaa ttcccagttc
     1561 ctccagcaac ggcagaattt cctgttcagg ctcacgccac cacatggaat attcgctttg
     1621 cagggcggtg acaggttgta ccgcatgtgc acgacgaatg gtttgtgctc ctgcttcaga
     1681 caggccgaaa tgtttgacct taccttctgc gatgaggtct ttcaccgttc ctgcaacatc
     1741 ttcaatagga acatctggat caacacggtg ttgatacagc aggtcaataa catctgtttt
     1801 cagacgacgt aatgatcctt caacggcttc acggatatgt tccggacggc tgtttaaaat
     1861 ctgctgctta ttatcatcgc cgaaggtaaa accgaattta gtggcaatta ccacgcggtc
     1921 acgaaacggt tttaaggctt cgccgaccac ttcttcattc agatacggac catacacttc
     1981 ggcggtatcg aagaaggtga caccacgttc aaccgcagca cgaataagtt cgatagcctg
     2041 acgggtatcg gtcgccgggc cgtagccgtg gcttaagccc atgcaaccga gcccaagggc
     2101 ggagacttcg agtccggatt tacccagata acgtttttgc attgaatgaa tacctctttt
     2161 atcgcggctt aaacatcaag tttgcggcca gtcagccatt ccaccattgc gggatcgcgg
     2221 tgagagaaga acgcgctggt tgcggtatcc agggcggtaa tttgcagcat atcttcaggg
     2281 ctgagttcaa aatcgagaat gttgatgttc tcttccatgc gttcttttcg cactgatttt
     2341 gccagtgaaa caatgcctcg ttggtagatc cagcgcagaa caacctggcc cacgcttttg
     2401 ccatacttct cgccaattgc cgtgagaaca ggatgctgga acagaccatt tttcccttcc
     2461 gcaaacggtg cccaggcttc tggctgaatg ccacgacttt gcatccaggg aacggcctgt
     2521 agctgctgat tgaaagggtt aacttcaacc tggtttaccg ccggaacgac gttattaaaa
     2581 gcgataaggt ctgccagtcg gtcaggatgg aagttactga cgccaatagc gcgaatttta
     2641 ccggcctgtt gcagctcttc catggcacgc caggctccgt ggacatcgcc atatggctgg
     2701 tgaatcaagt acaggtcaac gtaatcaagt tgcagtcgat tcagagagcg ttcgaactgg
     2761 gctttggccc cttcgtaact ggtatcctgc aaccagagtt tcgtggtgac aaagagttcg
     2821 ttacgggcga taccgctttg tttcagtgcg ttcccgacct ggatttcatt ttgataagag
     2881 gcggcggtgt caatcagtcg ataaccgctg ttaatagcgt caatgaccgc tctttcgcat
     2941 tcggcggcat cggtcatctg aaatacacca aagcccaaca ggggcatttc aataccgttg
     3001 ttcagtttta ctttttgcat ggtgttatcc ttccgttatc gttcatgaga agcataacgt
     3061 aaagggaaga gctcgattag acggcaggat ttgtcagggg ttatgaacaa aattcataaa
     3121 tctgtttgag ggttgagatt agtcgtgcaa gttcgatatc cccggaattt acagtccgat
     3181 gaataaaaat gaatgtccat aaaaaatata ttggtttatc ccggcgtata tacttacttc
     3241 gacgtactcc caatagcagt taattaaaga cgggtcatta ttattcactc tggataatga
     3301 attattcact gcccgcagag taagaaatat aacttaagta tctatttaat gacttgcaca
     3361 aaaagctaaa tttttaccca taaataaaaa tataatcccg cgcccaacca cctgatgagt
     3421 ggctacagac actggatata ttaggtggcg gtgtactttc ttacataaag gtatttcctt
     3481 ttctgtggaa aaggaaaccg ggaaatcccc ggtttttctg acaagcagac gccattattt
     3541 gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc acgttataaa
     3601 acagacaata aacagccacg atttcgttat tcagttctgg cccgctgcgt ggcgtgggca
     3661 aatatctctg ttcaggttct ttttccactc gcagtcacct ttacgccagt aatggcagca
     3721 cgtgcgcagc atgcggttca gccacggttg agcatggaaa atactacggt aactgctaat
     3781 aataacgtgg agaaaaatgt cgcgtcgctt gccgctaatg ccgggacatt tttaagcagt
     3841 cagccagata gcgatgcgac acgtaacttt attaccggaa tggccaccgc taaagctaac
     3901 caggaaattc aggagtggct cgggaaatac ggtactgcgc gcgtcaaact gaatgtcgat
     3961 aaaaatttct cgctgaagga ctcttcgctg gaaatgcttt atccgattta tgatacaccg
     4021 acaaatatgt tgttcactca gggggcaata catcgtaccg acgatcgtac tcagtcaaat
     4081 attggttttg gctggcgtca tttttcagaa aatgactgga tggcgggggt gaatactttt
     4141 atcgatcatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga atactggcgt
     4201 gattatttga aactgagcgc caatggttat atccgggctt ctggctggaa aaaatcgccg
     4261 gatgttgagg attatcagga acgcccggcg aatggctggg atattcgtgc tgagggctat
     4321 ttacctgcct ggccgcagct tggcgcaagc ctcatgtatg aacagtatta tggcgatgaa
     4381 gtcgggctgt ttggtaaaga taaacgccag aaagatccac atgcgattac cgctgaagtg
     4441 aattacacgc cagtgcctct tctgaccctg agtgccgggc ataagcaggg caagagtggt
     4501 gagaatgaca ctcgctttgg cctggaagtt aattatcgga ttggcgaacc tctggaaaaa
     4561 caactcgata cagacagcat tcgcgagcgt cgaatgctgg caggcagccg ctatgacctg
     4621 gttgagcgta ataacaatat cgttcttgag tatcgcaaat ctgaagtgat ccgtattgct
     4681 ctgcctgagc gtattgaagg caagggcggc cagacggttt ccctggggct tgtggtcagc
     4741 aaagcaactc acggtctgaa aaatgtgcaa tgggaagcgc cgtctttgct ggccgcaggc
     4801 ggaaaaatta cggggcaggg caatcagtgg caagtgacgc tcccggctta tcaggcaggc
     4861 aaagacaatt attatgcgat ttcagcgatt gcctacgata acaaaggcaa tgcctcgaaa
     4921 cgtgtgcaga cagaagtggt tattagcgga gctggtatga gcgccgatcg tacggcgtta
     4981 acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca aaagccgctg
     5041 gtgctgtctc tgcgcgacgc cgagggtcag ccagtcacgg gcatgaaaga tcagatcaag
     5101 actgaactaa ccttcaaacc ggctggaaat attgtgactc gtaccctgaa ggccactaaa
     5161 tcacaggcaa agccaacact gggtgagttc accgaaactg aatcaggggt gtatcagtct
     5221 gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt tgatgacatg
     5281 agcaaaactg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa ctccaccctg
     5341 agcgctaacg agccgtcagg tgatgtggtt gctgatggtc agcaagccta cacgctgaca
     5401 ctgacagcgg tggactccga gggtaatccg gtgacgggag aagccagccg cctgcgactt
     5461 gttccgcaag acactaatgg tgtaaccgtt ggtgccattt cggaaataaa accaggggtt
     5521 tacagcgcca cggtttcttc gacccgtgcc gggaacgttg ttgtgcgtgc cttcagcgag
     5581 cagtatcagc tgggcacgtt acaacaaacg ctgaagtttg ttgccgggcc gcttgatgca
     5641 gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttggcggtac agttacggca
     5701 atctggacgg caaaagatgc taatgacaac cctgtaactg gcctcaatcc ggatgcaccg
     5761 tcattatcgg gcgcagctgc tgctggttct acggcatcag gctggacgga taatggcgac
     5821 gggacctgga ttgcgcagat ttctctcggc actacggcgg gtgaattaga cgttatgccg
     5881 aagctcaatg ggcaggacgc ggcagcgaat gcggcaaaag taaccgtggt ggctgatgca
     5941 ttatcttcaa accagtcgaa agtctctgtc gcagaagatc acgtaaaagc cggtgaaagc
     6001 acaaccgtaa cgctggtggc gaaagatgcg catggcaacg ctatcagtgg tctttcgttg
     6061 tcggcaagtt tgacggggac cgcctctgaa ggggcgaccg tttccagttg gaccgaaaaa
     6121 ggtgacggtt cctatgttgc tacgttaact acaggcggaa agacgggcga gcttcgtgtc
     6181 atgccgctct tcaacggcca gcctgcagcc accgaagccg cgcagctgac tgttattgcc
     6241 ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaaactcc aacggttaaa
     6301 acgacgacgg aactcacctt caccgtgaag gatgcgtacg ggaatccggt caccgggctg
     6361 aagccagatg caccagtgtt tagtggtgcc gccagcacgg ggagtgagcg tccttcagca
     6421 ggaaactgga cagagaaagg taatggggtc tacgtgtcga ccttaacgct gggatctgcc
     6481 gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc tcagccactg
     6541 gtgctgaatg ttgcaggtga cgcatctaag gctgagattc gtgatatgac agtgaaggtt
     6601 aataaccaac tggctaatgg acagtctgct aaccagataa ccctgaccgt cgtggacagc
     6661 tatggtaacc cgttgcaggg gcaagaagtt acgctgactt taccgcaggg tgtgaccagc
     6721 aagacgggga atacagtaac aaccaatgcg gcagggaaag tggacattga gcttatgtca
     6781 acggttgcgg gagaacacaa tatttccgct tcggtgaatg gtgctcagaa gacggtcacg
     6841 gtgaaattca acgcggatgc cagcaccggt caggcaaacc tgcaggtaga caccgccgtt
     6901 caaaaagtgg caaacggcaa agatgccttt acgctgacgg cgaacgttga ggataaaaat
     6961 ggtaaccctg ttccagggag cctggtgacc tttaatctgc cccggggtgt caagccgctt
     7021 acaggcgata atgtgtgggt gaaagccaac gatgagggga aagcggagtt gcaggtggtt
     7081 tccgtgactg ccggaaccta tgagatcacg gcgtcagcag gaaatgacca gccttcgaat
     7141 gcgcagtctg taacgtttgt ggccgataag actacggcga ccatctccag tattgaggtg
     7201 attggcaacc gtgcagtggc ggacggcaaa accaaacaga cgtataaagt tacggtgact
     7261 gatgccaata acaacctgct gaaagatagc gaagtgacgc tgactgccag cccggaaaat
     7321 ttagttctga ctcccaatgg gacggcgaca acgaatgagc aagggcaggc tattttcacc
     7381 gccacgacca ctgtcgcagc gacatataca ctcacggcga aagtggaaca ggccgacggt
     7441 caggaatcga cgaaaactgc cgaatctaaa ttcgtcgcgg atgataaaaa cgcggagctc
     7501 gccgcaacat ctgatgtgca ttctctggtg gcggatgggg taacgacagc aacactgacg
     7561 gtaaccctgt tctcggcaaa caacccggta ggaggaacca tgtgggtcga cattgaggct
     7621 ccggaagggg tgacagaggc ggattatcag ttcctgccgt cgaaaaatga ccatttcgcg
     7681 agcgggaaaa tcacgcgtac atttagtacc aacaagccag gtacatacac attcacattc
     7741 aactctttga catatggagg gtatgaaatg aaacccgtga ctgtgacaat taacgccgtt
     7801 cctgcagata ctgaaggtgc tgaggagaaa taaaaacgac g
//
LOCUS       DAOFSV010000181         7700 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.181, whole genome shotgun sequence.
ACCESSION   DAOFSV010000181 DAOFSV010000000
VERSION     DAOFSV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.181"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..338
                     /gene="gadX"
                     /locus_tag="QBH27_004224"
     CDS             <1..338
                     /gene="gadX"
                     /locus_tag="QBH27_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDQ7016466.1"
                     /translation="IASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGV
                     SIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGT
                     DRSAEGIRL"
     gene            707..1435
                     /gene="gadW"
                     /locus_tag="QBH27_004225"
     CDS             707..1435
                     /gene="gadW"
                     /locus_tag="QBH27_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDQ7016467.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1798..4911)
                     /locus_tag="QBH27_004226"
     CDS             complement(1798..4911)
                     /locus_tag="QBH27_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDQ7016468.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4936..6093)
                     /gene="mdtE"
                     /locus_tag="QBH27_004227"
     CDS             complement(4936..6093)
                     /gene="mdtE"
                     /locus_tag="QBH27_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDQ7016469.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6153..6482
                     /locus_tag="QBH27_004228"
     CDS             6153..6482
                     /locus_tag="QBH27_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016470.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(6432..6959)
                     /gene="gadE"
                     /locus_tag="QBH27_004229"
     CDS             complement(6432..6959)
                     /gene="gadE"
                     /locus_tag="QBH27_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDQ7016471.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1994 a         1803 c         1829 g         2074 t
ORIGIN      
        1 gaattgctag cgaattgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag
       61 gtacgtcata ttcacagttg cttactgagt gtaggatgca gcgtgcttta caacttattg
      121 ttatatatgg ggtttcaatt aagcgagttg cagtatcctg tggatatcac agcgtgtcgt
      181 atttcattta cgtttttcga aattattatg ggatgacgcc cacagagtat caggagcgat
      241 cggcgcagga attaccgaac tgtggctcgg cggcaagtat ggctgcgcaa gggatttttt
      301 acggcactga ccgttctgcg gaaggaataa gattatagag ttttactcag acataaaaaa
      361 aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta
      421 taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac
      481 tggaaataag atcagccatt tttttataaa cataagctat acgctgtgtg aaaatataat
      541 cgtgttgcat ttacttatta tcaattaact gttatgcaaa actactttgt ggataaaatt
      601 tggtcctacc aaatatggca gtttttgcac acagaaacag tctggcgtca tttcattagt
      661 atactgacat tgaaataatc gcagtaatga aatataaggg atagtcatgg ctcatgtctg
      721 ctcggtgatc ctcgttcgtc gttcattcga tattcatcat gaacagcaaa aaatatcgtt
      781 gcataacgag agtatcctgc tgctggataa aaatttggca gacgattttg cgttttgttc
      841 actggatacg cgacggctgg atatcgaaga gctgacagtt tgccattact tacaaaatat
      901 tcgtcagttg ccacgcaatt taggattgca tagcaaagac cgtttgttaa ttaaccagtc
      961 accccccata cagctggtga cggcgatttt tgatagtttc aatgaccccc gggtcaattc
     1021 gccgatactg agcaaaatgc tctatctttc ctgtttatca atgttttctc ataagaaaga
     1081 actgatcccc ttacttttca atagtatcag tactgtttca ggaaaagttg aacgccttat
     1141 tagctttgat atcgctaaac gttggtatct acgcgatatc gcagaaagaa tgtacaccag
     1201 cgagagtctc atcaaaaaaa agttgcagga tgaaaatacc tgtttcagta aaatattact
     1261 cgcctccagg atgtcgatgg ccagacgatt actcgagtta cgtcaaatac ctctgcatac
     1321 tattgcggaa aaatgtggct atagcagtac gtcatacttt ataaatacat ttagacaata
     1381 ttatggtgta acgccacatc agttttcgca acattcgccg ggtacctttt cctgacatat
     1441 ttcgcatttg aatattggtc aggatctcac gtctgcttca tgtgaaactc tttcctgatg
     1501 atttctgccg ggctaccggc tagttctctt tcgcagtttg ccaggcggta taggtatttt
     1561 caggaggaaa agactcggca tgtttgggat tattaagcta acaattcata cgattacggg
     1621 aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat ggctggagtg gtttctactt
     1681 tcagtgctgt gtactaagcc tggtattcct gacggttagc tggttgttga gtggtgaatg
     1741 gttagcagga aagagtaagg ctgaaccctc acgttcaacc ttactctcat ttacacgtta
     1801 cgctttttta aagcgggcaa agagatgttc cactacaacg aaaaagaccg gaacgaagta
     1861 aattgccagc actgttgcgg caaacatccc gcccattacg ccggtaccta ccgcgttttg
     1921 cgcgccagag ccggcaccat gactgataac cagcggcagc acgccgagaa taaaggccag
     1981 agaagtcatc aggattgggc gtaaacgcat acgcgctgct tcgatgattg cctctaccgg
     2041 tgttttccct tctttctgca tcatctcaac ggcaaattcg acaatcagga tggcgttctt
     2101 ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag taaacgtcat tacttaagcc
     2161 gcgcagatcg gtggccagta atgcgccaac gacgcctaac ggaacaacca acatcaccga
     2221 gaacggaatt gaccagcttt cataaagtgc ggcgagggcg aggaacacca cgaccagtga
     2281 aatggcatat agcgcgggag cctgatttga ggataacgct tcctgatacg acagtccggt
     2341 ccatgagtag ccgacgcctg ccggaagttt tgcgaccagg tctgccataa atttcatggc
     2401 gtcaccggta cttttcccgg cagccgcttc acctaaaatc tccattgacg ggatgccgtt
     2461 gtagcgttcc agtcgcggtg aaccataggt ccattcggta gacgaatagg cagaaagcgg
     2521 tgccatcgtg ccagaggcgt tgcgtacata ccattggttg atgttatccg gcaacatacg
     2581 gaacggcgtg cctgcctgga catacacttt tttcacccgc ccctggttga ggaagtcgtt
     2641 cacgtagctg ctgccgaagg cggtggaaat tgtctggttg atatcagaca gcgccacacc
     2701 catagcctcg gctttcgcag cgttgacgtt cactttgaac atcggcgtat cttccaggcc
     2761 gttcgggcgt accccgatga cctgattcgg tgattgcgct gccagtgata acagctcgtt
     2821 tcgcgcctgg gtcagttttt cgtgccccag attgccgttg tccagcagtt ccatatcaaa
     2881 acctgacgcg gtacccagtt cagccaccgc gggtaagttg aacgggaaga cgacggcttt
     2941 attgatactg cttagcgcaa tcattgcccg ctgaataatc gcggtaaccg agttttcctc
     3001 gccgacacgt tcagaccacg gcttgaggct gataaacgcc agaccgttgt tttgcccctg
     3061 accactgaag ccaaagccac caacggtaaa caccgactga acattatttt tctcttttgt
     3121 cagataataa tccgtcacct gttgtagcac tttcgtggtg ttaaccatgg ttgcaccgga
     3181 aggtaactgc gcggtggtca taaatacccc ctgatcctct tctggtaaga aagaggtcgg
     3241 cgtacgcagg aacagcaccg ccatcccggc gcaaatcagt aggtagacca ccatgtagcg
     3301 accggtacaa cgcaacagcg agcgggtgct gtcggtatag tgctgagttg atttttcaaa
     3361 cagcgtgttg aagcgtgcga acagggcgtt aggtttgtga ccgccctccg gcgcggcttt
     3421 cagaatggtg gcgcacaggg caggggtcag gctcattgct acaaatactg aaagcagcat
     3481 ggaggagatc agcgtgatgg agaactggcg gtagatctcc ccggtcgcac cgctcataaa
     3541 ggccatcggc ataaacactg cggaaagaac aacggcaatc ccgaccagcg cacgttggat
     3601 ctgccccatc gatttatgcg tcgcttcctt cggcggtagc ttatcttccg caatgacacg
     3661 ctcgacgttc tccaccacca cgatggcgtc atccaccagt aaccctatcg ccagcaccat
     3721 cccgaacata gttaacgtgt tgatggtgaa accaactgcc gacaagatcg caaacgtccc
     3781 gagaataacc accggtacgg caatcgtcgg gatgattgtg gcgcggaaat tctgcaaaaa
     3841 cagatacatg accaggaaga cgaggatgat agcctcaacc agcgttttga aaacttcctg
     3901 aatagaaatt ttgataaacg gtgtggtgtc gtaaggataa accgtcttca ggcttgcagg
     3961 gaaataagcc gataaacggt tcagctcctc tttcaccgct cgcgaggtat ccagggcgtt
     4021 tgctccggta gccagtttga tggcgatccc ggcggcaggt ttgccgttat agcgcgccac
     4081 ggtggaataa tcttccgccc caagttcgac gcgggcgaca tcacgcagca gcacttgcga
     4141 accatcttgc tgaactttca acaggatttt gccaaattct tccggcgttt gcagacgcgt
     4201 ctgcacaatg atcgaggcgt tcagttgctg gtctgccgcc tgtggcatgc cacccagttg
     4261 accaccagaa atctggttgt tttgcacctt aatctgggaa ataacatcgg aaggtaccag
     4321 gttatattta ttgagttttt gcgggtccag ccagatacgc atggcgtact cggaaccaaa
     4381 gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg tctttgatat tagacgctac
     4441 atagtccgcg atatcgtact ggttgaggct gccgttatca gaaataaatg ccgctaccat
     4501 caggatatta ctgctcgact tatcgacgct aatcccctgc tgctgcaccg cttcaggtaa
     4561 tgaaggcata gcgagttgca gtttattttg cacctgaacc tgtgcgatat caggcgatgt
     4621 ccccgtctcg aaggtcagag tgatggaggc attacctgcc gcatcgctgg ttgaagacat
     4681 gtacatcagg ccatcaagcc cattcatatt ttgctcaatc acctgagtga ccgagtcttc
     4741 taccgtttgc gcatcggcac ccggataggt tgcgctgacg gtaatggtcg gtggcgcaat
     4801 ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga ccgcctgcaa gcatcataat
     4861 aatggcaagt acccaggcaa aaaccgggcg atcaataaaa tagttagcca tgaaagtccc
     4921 cttaagcctg caacgttatt gtttcgattc ggtgctggcg ttttcctggc tggaggaaat
     4981 tgctcgtgct ttgatacccg gacgaatgcg ttgcaagccg gaaacgatca cccgatcgcc
     5041 agcctgcaag ccagaggtga caacccactg gtcgccgatg gctttgctgg cttcgatttc
     5101 gcgtagctgc acgacatcgt ctttatccag aatgagtgcc gttgctttac cctgggcgtt
     5161 gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg cggctacctt catccactaa
     5221 tgccgtaaca tacatgccag gcagcaagtc accatttggg ttggggaaaa tcgcccgtaa
     5281 cgtcacggag cccgtggttt catccaccgt cgggtcggag aatttcagcg tgccggtctg
     5341 gccgtaacgt ttaccatttt ccagattgag ctgtactggc gtactgccct gaacctgttt
     5401 gatttgccca ctggcgacct cttctttcat gcgcaagaaa tcctgcaccg actgcgtgag
     5461 atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct gattagcggt
     5521 aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg gcgaggtgac
     5581 attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga cggtgacatt
     5641 ggcttctgct tcattcaact gggtgcgcgc ggtgtcgtaa tcctgacggc taacatagtt
     5701 ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg cggtagagag
     5761 cgctttcgcc agcgagcctt tggcggagtt cagctctgcc tgtaaaggcg caggatcaat
     5821 ctgatacagc gaatcgccct ggttcacttt atcgccttcg ataaagttgc gtttaatgat
     5881 aattccgccc acctggggac gtatctcggc aacttcataa ggaacggttc taccgggcaa
     5941 ttcgctcaac acattgaccg aaccggggga gagtgtgacg acaccgacct caggcgtcat
     6001 ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc cgcagaataa
     6061 caacggtatt aacagttttc ttcttctgtt cattttagtc cctgaaaatt cttgagaaat
     6121 aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc agcggcatag
     6181 tggggttgtc agccagtaag atgggatgca agcacagtga tcgacatggt gaggtcaacg
     6241 acaaaaaaaa tgttgccgtt ctgccaacag tttctgccag cattcgggca acgaagggtt
     6301 ctactggtgg atacacatac caggggaata aagtcgtttt cgtctctcct tcaataatca
     6361 ccgggggtat tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa tttcagttgc
     6421 ttatgtcctt actaaaaata agatgtgata cccagggtga cgatgtcgct catacgtctt
     6481 aatttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg cgaaatgttg
     6541 agcgttgacg tgatattgct ttcatttttg tttttcagaa tcagttgcat aattttgaac
     6601 tgattatgtg aaaaacatag ttgtgaattc ttatggggca agtgtttacc ataagtaatc
     6661 tcaagaagat ttttgatact ttctttacgg ctgacaaaat aaataggcgc tttagctttt
     6721 agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa tttctccagg
     6781 aatttaatcg cttcttcatc aaggatatgg ttgtgatagg tgtcaatgat caccttgaag
     6841 ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat ttcgtgatta
     6901 tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag aaaaatcata
     6961 acttgctcct tagccgttat cgtttcgaac atgtcatcct tttcattatt tacatccttg
     7021 tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg tgaactaata
     7081 cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat atattggtcc
     7141 taagtaagat tcctggttgt tatcagtttg taactagtaa acaaaaggtt aacttttgcg
     7201 gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag aatattacta
     7261 aagttaaaat gtaatccgat ttaaatatcg agtctccttg tttcgactta ggctggcaat
     7321 tggattgcca gctttctttt aacgcattga aattacctga ttaaataact cattcatgtc
     7381 ttcaggtttt tatatagcgt ctggagacac ggatatttat gcaatgtaat caacgccagt
     7441 tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat agcatcaacg cttacggaac
     7501 tctttgttca ctttcccaca gcacttttaa aacattaaac ctgaccacgg aaaatatcag
     7561 ccatgataat gtttgaaatg gctaatttgc catggaacga aaaaaatcag attaaattca
     7621 gtaggttgaa ataatcaaca gcaggcaatt gtttcaatga tagtgcgtaa ttgaactgaa
     7681 acactgtata gctaagagag
//
LOCUS       DAOFSV010000182         7565 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.182, whole genome shotgun sequence.
ACCESSION   DAOFSV010000182 DAOFSV010000000
VERSION     DAOFSV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.182"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(2..2857)
                     /gene="valS"
                     /locus_tag="QBH27_004230"
     CDS             complement(2..2857)
                     /gene="valS"
                     /locus_tag="QBH27_004230"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDQ7016472.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(2857..3300)
                     /gene="holC"
                     /locus_tag="QBH27_004231"
     CDS             complement(2857..3300)
                     /gene="holC"
                     /locus_tag="QBH27_004231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDQ7016473.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3656..5167)
                     /gene="pepA"
                     /locus_tag="QBH27_004232"
     CDS             complement(3656..5167)
                     /gene="pepA"
                     /locus_tag="QBH27_004232"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDQ7016474.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5434..6534
                     /gene="lptF"
                     /locus_tag="QBH27_004233"
     CDS             5434..6534
                     /gene="lptF"
                     /locus_tag="QBH27_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDQ7016475.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6534..>7565
                     /gene="lptG"
                     /locus_tag="QBH27_004234"
     CDS             6534..>7565
                     /gene="lptG"
                     /locus_tag="QBH27_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDQ7016476.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQIDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLP"
BASE COUNT         1597 a         2067 c         2046 g         1855 t
ORIGIN      
        1 attacagcgc ggcgataaca gcctgctgtt caatcagctt cgctttcgct tccgcatagc
       61 cttccagctt ctcacgctct ttcgcgatga ccgcttccgg tgcgcgggcg acaaagcctt
      121 cattcgccag cttgttctcg atgcggctga tttcgccttc gatcttcgcc acttctttcg
      181 ccagacgcgc cagctcatct tctttgttga tgaggccagc catcgggatc agcagctctg
      241 cgccgtcgat aatcttcgta acggaaaccg gacctttgtc atcggcaggc agcacggtga
      301 tgctttccag acgcgccagg gtttgcagga agccacggtt ttcatttacg cgacgttctg
      361 catccgcgct gcaaccacgc agcagcagct ccagcggttt gcccggcgcg atgttcattt
      421 ctgcacggat gttacgtacc gcaacgatcg cctgcttcag ccactcggtg tcggccagcg
      481 ccgcttcatc aacctgagat gcatcgtact gcgggaacgg ctgcagcatg atggtgtcgg
      541 cagtgatacc gcaaagtact ttcacacgct gccagatggt ttcggtgatg aacggaatga
      601 tcggatgcgc gaggcgcagc agaccttcca gtacagtcac cagcgtatgg cgagtaccgc
      661 gcagttcggc ttcggttcca ccgttcatta ccggcttggt cagctccaga taccagtcgc
      721 agaactggtt ccaggtgaac tcatacagaa tgcctgcagc gatatcgaag cggaagctgt
      781 ccagcgcttc gcggtacgct ttgatggtct ggttgaactc tgccagaatc cagcggtccg
      841 ccagcgacag cgtcatttca ccaccgttga agccgcaatc ctgaccttcc gtgttcatca
      901 gcacaaagcg gctggcgttc cacagcttgt tacagaagtt acggtaacct tccaggcgct
      961 tcatatccca gttgatgtca cgaccggtag acgccagcgc cgccagggtg aagcgcaggg
     1021 catcggtgcc gtgcggctca atgccgttcg ggaactgttt ctcggtgcgc ttacggattt
     1081 tgtccgccag ctgcggctgc atcatattgc cggtacgttt ttccagcagt tctggcagcg
     1141 aaataccgtc aaccatatcc agcgggtcga taacgttacc cttggatttg gacatcttct
     1201 ggccttcgtc gtcacgaatc agaccggtca tgtaaacagt gtggaacggc acctgcggtt
     1261 tgccattttc atctttgatg aagtgcatgg tcatcatgat catgcgggca atccagaaga
     1321 agatgatgtc gaagccggat accatcacgc tggttgggtg gaactgacgc agggcgtcgg
     1381 tgttttccgg ccagccaagg gtagagaagg tccacagtgc agaagagaac caggtgtcga
     1441 gaacgtcttc gtcctgacgc agggcaacgt cggcaccgag gttattttcc ttgcgcactt
     1501 cttcttcgtt gcggccaaca taaacgttac ctgcttcgtc gtaccatgca gggatacggt
     1561 gaccccacca cagctgacga gagatacacc agtcctgaat atcgcgcatc caggagaagt
     1621 acatgttttc gtactgcttc ggtacgaact gaatgtcgcc gttctcaacc gcttcaaccg
     1681 ccggtttcgc cagtacatcg gcacgcacgt accactggtc ggtcagcatt ggttcgataa
     1741 ctacgccgcc acggtcgccg taaggaacgg tcaggtcgtg cggtttaatt tcttccagca
     1801 ggccgagtgc gtcaaccgca gcaacgactg ctttacgtgc agcaaaacgc tcgagtttct
     1861 ggaactctgc cgggatttcg ctggaataaa cgtcagattc gttacctttg gtatcgaaca
     1921 cctgggcgct ttcacggata tcgccgtcaa aggtcaggat gttgatcatc ggcagcgcgt
     1981 gacgtttacc cacttcatag tcgttaaagt cgtgcgccgg agtgattttc acgcagccgg
     2041 tgcctttttc catgtcggcg tgttcgtcgc caacgatcgg aatacgacgg ttaaccagcg
     2101 gtagaatgac atatttgcca atcaggtctt tgtaacgcgg atcttccggg ttaacggcca
     2161 cgccggtatc gcccaacagg gtttccggac gggtagtcgc aaccaccaga taatctttac
     2221 cgtctgcggt tttcgcgccg tcagccagcg gatagcggat gtgccacatc gaacctttcg
     2281 attcgcggtt ttccacttcc aggtcagaga tagcggtgcg cagtttcgga tcccagttta
     2341 ccaggcgttt gccacggtaa atcaggtctt ctttatacag acgaacgaaa acttctttca
     2401 ccgcattgga caggccttcg tccatcgtga agcgttcacg ctcccagtcg acggagttgc
     2461 cgagacggcg catctgacgg gtaatcgtgc cgccggattc cgctttccat tcccagattt
     2521 tgtcgatgaa agcttcgcgg ccgtagtcgt gacgggtttt accttcttct gcggcaatct
     2581 tgcgctcaac gaccatctgg gtagcgatcc cggcgtggtc agtaccgacc tgccacaggg
     2641 tgtttttgcc ctgcatgcgc tgatagcgga tcatggtatc catgatggtt tgctggaagg
     2701 cgtgacccat atgcaaactg ccggtgacgt tcggcggcgg gatcatgatg cagaaacttt
     2761 cctggctttc atcgccatta ggcttaaagt agccctgctt ttcccagtgc tcgtaaagcg
     2821 gctgttcgat atcttgtggg ttatatgtct tttccattat ttccaggttg ccgtattcag
     2881 gttgaaacca gccacgcggt aggctttata gcgttcgcgc gccagttgtt tcagagaatc
     2941 ttcataagga acgaagtcta ccacttctgt gaaagcggtg gcaaaatctg caaagcttgt
     3001 tcgcagacta atcaatatat cgcgcgggct gctgctgcgt ttttgcggcc aggcgatctc
     3061 caccggcgca ccgccgcgcg gtccttctcc cgctaaatta tgcggaacaa agctttctgc
     3121 cggacgcgcc cacagggctt catccagccg gtaagcctgc ttttcatctt cacaggcgat
     3181 gagcacgcgc ttaccgctgc gccaacgttc tgcggcaatt tcacacacca gttgctcaac
     3241 ggcgcttaag ccatcgacgg tggtgtcatt gtccagaagg tagaacgtcg cgtttttcat
     3301 aaatggggct tcttgtggtg gatttaaata cgcatcaggt atacaccgca gcgcctgatg
     3361 cgacgctgtt gtgtcttatc aggcctacga gttcagtgct gtgtgtaggt cggataaggc
     3421 gctcacgccg catccgacaa taacagcatt gcctgatgcg acgctgttgt gtcttatcag
     3481 gcctacgagt tcagtgctgt gtgtaggtcg gataaggcgt tcatgccgca tccgacaata
     3541 acagcattgc ctgatgcgac gcttgcgcgt cttatcaggc ctacgagttc agtgctgtgt
     3601 aggtcggata aggcgttcac gccgcatccg gcaataacag ccttgcctga cgcaattact
     3661 cttcgccgtt aaacccagcg cggtttaaca ggaactgtgc cagcaacgcc accgggcgac
     3721 cggttgcgcc ttttgcttta ccagaacgcc aggcggtacc ggcgatatcc aggtgcgccc
     3781 agttgtactt acgggtaaag cgtgacagga agcaacctgc ggtaatcgcc ccaccaggac
     3841 gaccgccaat gttcgccata tcggcaaaat tggactccag ttgttcctga tactcgtcac
     3901 ccagcggtaa gcgccatgcg cggtcaccgg attgttcaga cgcggcaatc agttcatggg
     3961 ccagcggatt atggttcgcc atcagaccag taatatgatg acccagcgcg atcacgcagg
     4021 caccggtcag cgtcgccacg tcaatcaccg cttccggctc aaaacgctca acgtaagtta
     4081 acacgtcgca cagtaccagg cggccttcag cgtcagtatt cagcacttca acggtttggc
     4141 cggacatggt ggttaacaca tcgcccggac gataggctcg tccgccaggc atgttttcgc
     4201 agcctgccaa cacgccgata acgttaatcg gcagttgtag ctccgcgacc atacgcatca
     4261 caccgtaaac cgctgccgca ccgcacatat cgtacttcat ctcatccatg ccttctgaag
     4321 gcttgatcga gataccgccg gagtcgaagg ttaaaccttt acccaccagc acgattgggc
     4381 gtgcatcttc cgacgcgttg cctttgtact caatcaccga catcagcgat tcgttttgcg
     4441 aaccctgacc gaccgccaga taggaatgca tccccagctc tttcatctgc tgttcgccga
     4501 taacgcgggt gataacattc ttgctgtagc tgtcagccag ctggcgcgct tgtgaagcga
     4561 ggtaagcggc gttacagata ttcggcggca tattgccaag atcctttgct gctttaatcc
     4621 cggcggcaat cgccagaccg tgctggatcg cgcgctcacc gctggtcagt tcacggcggg
     4681 tcggcacgtt gaacaccatt ttacgcagcg gacgacgcgg ttcgctcttg ttcgttttca
     4741 gctgatcgaa actgtagagc gtctcttttg ccgtctcgac agcctgacgc actttccagt
     4801 agttgttacg gcctttaacg tgcagttcag tcagaaagca gaccgcttcc attgagccag
     4861 tatcattcag cgtattaatg gttttctgaa taacctgctt gtactgacgc tcatccagct
     4921 cacgttcttt gccgcaacca ataaggagga tgcgttcaga aagcacgttt ggtacatggt
     4981 gcagcaacaa tgtctgcccc ggttttcctt ccagttcgcc ccgacgtagc agggcgctga
     5041 tgtacccatc gctgatttta tcgagctgtt ctgcaatcgg agaaaggcga cgtggttcga
     5101 agacgcccac gacgatgcag gcactccgct gtttctccgg gctaccgctt tttacactaa
     5161 actccatgca ctacgctcct gaatcttaaa gacaacggcg gtggctacag atagaattgc
     5221 aagctttcgt aactcatgtc cgctgttgcg atgacttcgt gttaatctta acgttattac
     5281 ggcattggca cgtcagaaca aaatctgaga ggtgaatccg ttgagtataa ttatcttagc
     5341 gacgatttcg acgactcaag agaataaatg acgtttaagc catgaaacaa gctaaaatcc
     5401 tgcaaaagac gagtttttac gggcgtattt aaagtgataa tcataagata tctggtgcgg
     5461 gagacgctca aaagccagct ggcgatactc ttcatcttgc ttttgatctt cttctgtcaa
     5521 aagttagtga ggatcctcgg cgcagcggtt gacggcgata ttccggcgaa tctggtgctc
     5581 tcccttctcg ggttgggcgt gccggaaatg gcgcagctta tcctgccatt aagcctgttc
     5641 ctcgggctgc tgatgacgct gggcaaactg tataccgaaa gtgaaattac ggtaatgcat
     5701 gcctgcggcc tgagcaaagc ggttctggtg aaagcggcaa tgatccttgc ggtattcacg
     5761 gcaatcgtcg cggcggttaa cgtgatgtgg gcgggaccgt ggtcatcgcg tcatcaggat
     5821 gaagtgttag cagaagcgaa agcgaaccct ggcatggcgg cgctggcgca agggcaattc
     5881 cagcaagcga ctaatggcag ctcggtgctg ttcatcgaaa gcgttgacgg cagcgatttc
     5941 aaagatgtgt tcctcgcaca aattcgacca aaaggtaatg cacgaccttc tgtggtggtg
     6001 gccgattccg gacatttaac ccagctgcgc gacggctccc aggtcgtcac tctcaaccag
     6061 ggaacgcgct tcgaaggcac tgcactgtta cgtgatttcc gcattacgga tttccaggat
     6121 tatcaggcga tcattggtca ccaggcggtg gcgctcgacc cgaacgatac cgaccagatg
     6181 gacatgcgca cattgtggaa cactgacacc gatcgtgccc gcgcagaact gaactggcgt
     6241 atcacgttgg tattcaccgt gtttatgatg gcacttatgg ttgtaccgtt gagcgtggtt
     6301 aacccgcgtc agggacgcgt actgtcgatg ctgccagcca tgctgctgta tctacttttc
     6361 ttcctgatcc agacctccct gaaatcgaac ggcggtaaag gtaagctgga cccgacgctg
     6421 tggatgtgga ccgttaacct gatttatctg gctttggcga ttgttctcaa cctttgggac
     6481 actgtgccgg tccgccgcct gcgcgccagt ttttcgcgta aaggagcggt gtgatgcaac
     6541 cttttggcgt acttgaccgc tatatcggta aaactatttt caccaccatc atgatgacgc
     6601 tgttcatgct ggtgtcgctg tcgggcatta tcaaatttgt cgatcagttg aaaaaagccg
     6661 ggcaggggag ctacgacgcg ttaggcgcag gaatgtatac cttgctgagc gtgccgaaag
     6721 atgtgcaaat attcttcccg atggcggctt tgcttggggc gttgcttggt cttgggatgc
     6781 tggcgcagcg cagcgaactg gtggtgatgc aggcttctgg ttttacccgt atgcaggtgg
     6841 cgctgtcggt gatgaaaacc gccattccgc tggtcttgct gacgatggcg attggcgaat
     6901 gggtcgcgcc gcagggcgag cagatggcgc gtaactaccg tgcgcaggcg atgtacggcg
     6961 gctcgttgct ctctacccag caaggtttat gggcgaaaga tggcaacaac ttcgtctaca
     7021 ttgagcgggt taaaggtgac gaagagttag gtggcatcag tatttatgcc tttaacgaga
     7081 atcgtcgtct gcaatccgta cgctatgccg ctaccgcgaa gtttgacccg gaacataaag
     7141 tctggcgtct gtcgcagatt gatgaatctg atctgaccaa tccgaaacag atcaccggtt
     7201 cgcagacggt gagcggcacc tggaaaacca acctcacgcc tgacaaactg ggcgtggtgg
     7261 cgctggatcc ggatgcactc tctatcagcg gtttgcacaa ctatgtgaag tatctgaaat
     7321 cgagcggtca ggatgccgga cgttatcagc tcaacatgtg gagcaaaatc ttccagccgc
     7381 tatccgtggc ggtgatgatg ctgatggcgc tgtcgttcat ctttggccca ctgcgtagcg
     7441 taccgatggg tgtgcgtgtg gtcaccggta tcagcttcgg ttttgtcttc tatgtactgg
     7501 accagatctt cggcccgctg acgttggttt atggcatccc gccgatcatc ggcgcactgt
     7561 tgcca
//
LOCUS       DAOFSV010000183         7561 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.183, whole genome shotgun sequence.
ACCESSION   DAOFSV010000183 DAOFSV010000000
VERSION     DAOFSV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.183"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            92..907
                     /gene="gspC"
                     /locus_tag="QBH27_004235"
     CDS             92..907
                     /gene="gspC"
                     /locus_tag="QBH27_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDQ7016477.1"
                     /translation="MPTLPFPFHLANHNKDAAINILITFISIGLIIFNVNYFHTTIVK
                     NEQIINQPTNAFQSEFSLAALWRNENHTGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEISQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            891..2843
                     /gene="gspD"
                     /locus_tag="QBH27_004236"
     CDS             891..2843
                     /gene="gspD"
                     /locus_tag="QBH27_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDQ7016478.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2853..4334
                     /gene="gspE"
                     /locus_tag="QBH27_004237"
     CDS             2853..4334
                     /gene="gspE"
                     /locus_tag="QBH27_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDQ7016479.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQTVIRGDTSWDEVMRVASATLENKA"
     gene            4331..5527
                     /gene="gspF"
                     /locus_tag="QBH27_004238"
     CDS             4331..5527
                     /gene="gspF"
                     /locus_tag="QBH27_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDQ7016480.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5537..5974
                     /gene="gspG"
                     /locus_tag="QBH27_004239"
     CDS             5537..5974
                     /gene="gspG"
                     /locus_tag="QBH27_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDQ7016481.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5982..6491
                     /gene="gspH"
                     /locus_tag="QBH27_004240"
     CDS             5982..6491
                     /gene="gspH"
                     /locus_tag="QBH27_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDQ7016482.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6488..6865
                     /gene="gspI"
                     /locus_tag="QBH27_004241"
     CDS             6488..6865
                     /gene="gspI"
                     /locus_tag="QBH27_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDQ7016483.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6858..7445
                     /gene="gspJ"
                     /locus_tag="QBH27_004242"
     CDS             6858..7445
                     /gene="gspJ"
                     /locus_tag="QBH27_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDQ7016484.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7438..>7561
                     /locus_tag="QBH27_004243"
     CDS             7438..>7561
                     /locus_tag="QBH27_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein K"
                     /protein_id="HDQ7016485.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQV"
BASE COUNT         2013 a         1931 c         1827 g         1790 t
ORIGIN      
        1 aattttgctt gattcatgca agcggcatta atactattta tactaacgtc aatatacaac
       61 ccacccaatc tattttatta gaacatacat cgtgcccaca ctacctttcc ccttccatct
      121 ggcgaatcat aataaagatg ctgccatcaa tatattgatt acatttataa gcatagggct
      181 gattattttt aatgtgaatt atttccatac aactatagtt aaaaatgaac agatcattaa
      241 tcaaccaaca aatgcatttc agtctgaatt cagccttgcc gctttatggc gcaacgaaaa
      301 tcatactggg gtaaaagatg caaatcccgt ggcagttaat caggaaacac ctaagctctc
      361 tatagcgctt aatggcattg tactcacatc gaatgatgag acttcttttg tcttaattaa
      421 tgaggggaac gaacaaaagc gatactccct caacgaagcc cttgagtcag cgccgggaac
      481 gtttattaga aaaataaata aaaccagcgt tgtgtttgaa actcacgggc attacgaaaa
      541 agtaactctc catccagggc tacctgacat catcaagcag cctgattcgg aaaatcaaaa
      601 tgtactcgcc gattatatta tcgccacacc tatacgtgat ggagaacaga tatacggtct
      661 tcgattgaat cctcgtaagg gtctcaatgc atttaccacc agtttgctgc aacctgggga
      721 tatcgccctc aggatcaata atctttcgct gacccaccct gatgagattt cacaggcatt
      781 aagtttactg ctaactcaac aaagtgctca gtttacaatt cgtcgcaacg gcgtaccccg
      841 cttgataaat gtttccgtcg cggaacttac aggaatgaat ggacagcgca atgaagggac
      901 tcaataaaat cacctgctgc ttgctggcag cattactcat gccttgtgca ggacacgctg
      961 agaacgaaca atacggcgct aacttcaata acgccgatat ccgccagttc gtggaaatag
     1021 tgggtcagca tcttggcaaa acgatcctga tcgacccttc ggtacaggga accatttccg
     1081 tacgcagtaa tgatacgttt agccaacagg agtactacca gttcttttta agtattcttg
     1141 atctttacgg ttattccgtg atcacgctgg acaatggttt tctgaaagtg gtgcgctcag
     1201 ctaatgtaaa aacatcgcca gggatgattg ctgacagttc tcgtccaggc gtaggtgatg
     1261 agttggtcac ccgaatcgta ccgcttgaga acgttcctgc tcgtgacctg gctcccctgc
     1321 tccgccagat gatggatgcg ggtagcgtcg gtaatgttgt gcattatgaa ccctccaacg
     1381 ttcttattct gaccggtcgt gcctccacca ttaataaact gattgaagtc ataaagcgcg
     1441 ttgatgtcat cggcacagag aagcagcaaa ttattcatct ggaatatgca tcagcggaag
     1501 atctcgccga gattcttaat caattaatca gcgaaagcca cggtaaaagc cagatgcctg
     1561 ccctcctttc cgcgaagatt gtggcggata agcgaaccaa ctctcttatc atcagcggac
     1621 cggaaaaagc acgccagcgc ataacttcat tactgaaaag ccttgatgta gaagagagcg
     1681 aggaaggaaa tacccgggtc tattacctga aatatgctaa agccacgaat ctggtggaag
     1741 tgctaaccgg tgtttccgaa aagctgaaag atgaaaaagg gaattcgcgt aagccttctt
     1801 ctacttctgc gatggataat gtggccatta ccgccgatga acagaccaac tcgttggtca
     1861 ttaccgctga ccagtccgtc caggagaaac tcgccacggt aattgcgcgt ctggatattc
     1921 gccgtgcaca ggtgctggtt gaggcaatca tcgtcgaagt tcaggatgga aatggactca
     1981 acctcggcgt gcaatgggcg aataaaaacg ttggcgcaca gcaatttacc aatacgggat
     2041 taccggtttt taacgctgcg caaggtgtag ctgattataa aaagaatggc gggatcacca
     2101 gcgcgaatcc tgcctgggat atgtttagcg cctacaatgg catggctgca ggcttcttca
     2161 atggcgactg gggcgtattg ttgactgcgc tggccagtaa caataaaaat gacatcctcg
     2221 ccaccccgag catcgtaacg ctggataata aactcgcgtc cttcaacgtt ggtcaggatg
     2281 tgccggtgct atccggctct cagaccactt ctggggataa cgtctttaat actgtcgaac
     2341 gcaaaacggt gggcacaaaa ctcaaagtca ctccgcaggt caatgaaggc gacgcggtac
     2401 tgctcgaaat agaacaggag gtttccagcg ttgactcttc gtctaattcg acgctcggcc
     2461 cgacatttaa cacccgtact attcaaaacg ccgtgctggt taaaaccggt gaaacggtgg
     2521 tcctgggcgg attgctggat gatttttcta aagagcaagt gtcaaaggtt cctctgcttg
     2581 gcgatattcc tttagtggga caactcttcc gctatacctc gaccgagcgc gctaaacgca
     2641 acctgatggt attcatccgc ccgacgatta tccgtgacga tgatgtttat cgctcactgt
     2701 caaaagagaa atacacccgc taccgtcagg agcaacagct gcgaatcgac gggaaatcaa
     2761 aagcgctggt tggctcggaa gatttgccgg tgctgaatga gaacacgttc aacagtcacg
     2821 cccctgcgcc atcgtcacgg tgaggcattc ttatgagaat tcactcaccg taccccgcca
     2881 gttgggcgct ggcacaacga attggttatc tctattcaga gggcgagatt atttatctcg
     2941 ccgatacgcc attcgaacgg ttactcgata ttcaacgtca agttggtcag tgccagacca
     3001 tgaccagctt gtcacaggct gattttgaag cccgactgga agcggtgttc catcagaata
     3061 ccggtgagtc gcaacagatt gcgcaggata tcgatcaatc cgtcgatctt ctctcgcttt
     3121 cggaagagat gcccgcaaat gaagatctcc tgaatgaaga ttcagcggca ccggttatcc
     3181 gcttgatcaa tgctattttg agtgaggcca tcaaagaaac cgcctccgat atccacattg
     3241 aaacctatga aaaaacaatg tcgatccgtt ttcgcatcga cggcgttttg cggacaattt
     3301 tacagcccaa caaaaaactg gcggcactgc ttatctcccg aattaaggtc atggctcgtc
     3361 ttgatatcgc cgaaaaacgt attccacagg atggaaggat cagtttgcgt atcgggcgac
     3421 gtaacataga tgtccgcgta tccacactgc catccatcta tggtgaacgc gctgtgctcc
     3481 gtctgctgga taaaaacagc ctccagcttt cattgaacaa cctggggatg acggcagcgg
     3541 ataaacagga tttagaaaat ctcattcagc ttccgcacgg tattatcctg gtgacagggc
     3601 cgacaggctc cggtaaaagc accacgctct acgccatcct ttcggcgctt aatatccctg
     3661 gccgtaatat tctgacggta gaagatcccg tggaatatga gctggaaggc attggacaaa
     3721 cgcaggtgaa tacccgtgtg gatatgtctt tcgctcgcgg cctgcgcgcc atacttcgcc
     3781 aggacccgga tgtcgtcatg gtgggggaaa ttcgtgatac agaaaccgcg caaattgcgg
     3841 ttcaggcctc gctcacaggc catctggtac tctcaacact ccacactaac agtgcatcag
     3901 gcgcagtcac ccggctacgc gacatgggcg tcgaatcatt cctgctttca tcttcactgg
     3961 cagggattat cgcgcaacgt ctggttcgtc gtctgtgtcc gcaatgccga caattcacgc
     4021 ccgtatcgcc ccagcaagcg cagatgttta aatatcatca gctcgcggtg acaacaattg
     4081 gcactcccgt aggttgccct cattgccatc aatccggtta tcaggggcgc atggcgatcc
     4141 acgaaatgat ggtggtgacg ccggaattgc gggccgctat tcatgaaaat gtggatgaac
     4201 aagcactgga gcgactggtc cggcaacaac acaatgcctt aatcaaaaat ggcctgcaaa
     4261 cagtgatacg cggtgacacc tcctgggatg aggttatgcg cgtcgccagt gctacgctgg
     4321 agaacaaagc atgaattatc gctatcgcgc catgacccag gatggtcaaa aattgcaggg
     4381 gatcattgat gctaacgatg aacgtcaggc acgactgcgg ctgcgtgaag aagggctttt
     4441 cctgttggat attcgccccc aaaaaagttc gggagtaaaa acacgtcgcc cgaggatcag
     4501 ccatagtgaa ctgacgcttt tcacccggca gttggcaacc ttaagcgcag cggcattacc
     4561 cctggaagag agccttgccg taattggtca acaaagcagt aataaacgac tggctgacgt
     4621 gttaaatcag gtacgcagcg ccatccttga agggcatccc ctttcagatg cattacagca
     4681 tttccccacg cttttcgatt cgctctaccg taccctggta aaagcgggcg aaaagagcgg
     4741 tctgctggcc ccggtgttgg aaaagctggc tgattacaat gaaaaccggc agaaaatccg
     4801 cagcaagctc attcagtcac tgatctaccc ctgcatgctc actacggtgg cgattgtggt
     4861 cgtgattatt ctcctcactg ctgtcgtgcc caaaattacc gaacagttcg tgcatatgaa
     4921 gcagcaactg ccactgagta cacgcattct tttaggtctg agcgacacgt tgcaacgtac
     4981 cggcccgaca ttattagcga cagtatttat tgtcgctgtg ggtttctggc tctggttaaa
     5041 acgcggcaat aaccgccacc gttttcatgc catgttactg cgcgttgcgc tcatcggccc
     5101 gctgatttgc gccattaaca gcgcacgcta tctccgcact ttaagtattt tgcaatccag
     5161 cggcgtccct ctgctggatg ggatgaattt gtccaccgaa agcctcaaca acctcgaaat
     5221 tcgccagcgc ctggcaaatg cggcagaaaa cgttcgccag ggtaacagca ttcatctttc
     5281 actggaacaa accgcaattt tcccgccgat gatgctctac atggtggcct ctggcgaaaa
     5341 aagcgggcaa ctcggcacgt taatggtcag agccgcagat aaccaggaga cactccaaca
     5401 aaatcggatc gccttaacgc tctccatctt cgagccagca ctcattatta cgatggcact
     5461 gatcgtcctg tttattgtcg tgtcggtact ccaacctctt cttcaactta actcaatgat
     5521 taattaagga aatcatatgc gcgcaacgga taagcaacgc ggttttacat tactggaaat
     5581 tatggtggta attgtcatca ttggcgtact cgccagcctg gtggttccca acctgatggg
     5641 caataaagaa aaggcggata aacaaaaagc cgtcagcgat attgtggcgc tggaaaacgc
     5701 cctggatatg tacaagctcg acaatcatcg ctatccaacc acaaatcagg ggcttgaatc
     5761 cttagtagaa gcgccgacac tgccaccgct ggccgcaaac tataacaagg aaggttatat
     5821 caagcgtctg cctgccgatc cctggggcaa tgattatgtc ctcgttaatc ctggtgaaca
     5881 tggtgcatac gatctgcttt cagcaggacc cgatggtgaa atgggaaccg aggacgacat
     5941 caccaactgg ggtttgagca agaagaaaaa gtaagtcagc gatgaatcag caacgcgggt
     6001 ttaccctgct ggagatgatg ctggtgctgg cgctggtcgc tatcacggca agtgtggtgc
     6061 tcttcacata tggtcgggag gatgccgcca gcactcgtgc gcgcgaaaca gcggcgcggt
     6121 ttaccgctgc acttgaatta gccatcgacc gcgccacgct cagtggtcag cctgtcggta
     6181 tccatttctc tgattcagca tggcgcatta tggtaccggg caaaacacca tcagcctggc
     6241 gctgggttcc attacaagaa gatgccgcag acgagagcaa aaatgactgg ggtgaagaac
     6301 tctcaattca acttcagcca tttaaacctg atgacagcaa ccaaccgcag gtggtgattc
     6361 tggccgacgg acaaatcacg cccttctctt tgttgatggc taataccggg accggtgagc
     6421 cgctcctgac gttagtcagt tcaggctcat ggcccctgga tcaaacgctc gccagagata
     6481 cccgcccatg aacaaacaat cagggatgac actgcttgaa gtcttactgg cgatgagtat
     6541 tttcaccgct gttgcgctga ctttaatgag cagtatgcaa gggcaacgaa ctgccatcga
     6601 gcggatgcgt aacgagacgc tggcattgtg gatagcggat aaccagcttc agtcacaaga
     6661 ttcattcgac gaggaaaaca cgtccagttc tggcaaagag ttaataaatg gcgaagagtg
     6721 gaactggcgc agcgatatcc actcaagcaa agacggcacg cttctggaac gcaccataac
     6781 ggtgacatta cccagcggcc agacaacctc gcttacgcgc tatcaaagca tcaataataa
     6841 atccgggcag gcacaagatg attaatcgcc agcagggttt cacattgctg gaagtcatgg
     6901 cggcgctggc gattttctca atgcttagcg tgctggcgtt tatgattttt tctcaagctt
     6961 ccgagttgca tcaacgttca cagaaagaaa tccagcagtt caatcagcta cagcgcacca
     7021 tcaccattct tgataacgat ctcctgcaac tggtcgcccg gcggaatcgg agtacggaca
     7081 aaatcatggt gttgggtgaa gaagcgattt ttaccacgca aagtcgcgat ccgctggccc
     7141 ctctcagtga agcacaaacc ttactcactg ttcactggta tctgcgaaat catacgctct
     7201 accgtgctgt tcgtacttct gtggatggca ggaaggatca acccgcccag gcgatgctgg
     7261 aacatgtcga gagctttctt ctggaaagta acagcggaga aagccaggag cttcctctct
     7321 cagtcacgtt acatctgaag actcagcaat atggggcgtt acagcgccgt tttgcattac
     7381 ctgagcagtt agcgagagaa gagtcgccag ctcagacgca ggccgggaat aataaccatg
     7441 aataatgaac aacgcggcgt agcactgctc attgtactca tgttactggc gctcatggct
     7501 gctctggctg cagacatgac gatcagtttt cacggccagc ttcatcgaac ccgccaggta
     7561 a
//
LOCUS       DAOFSV010000184         7507 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.184, whole genome shotgun sequence.
ACCESSION   DAOFSV010000184 DAOFSV010000000
VERSION     DAOFSV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.184"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..126)
                     /gene="sra"
                     /locus_tag="QBH27_004244"
     CDS             complement(<1..126)
                     /gene="sra"
                     /locus_tag="QBH27_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDQ7016486.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRP"
     gene            complement(228..443)
                     /gene="bdm"
                     /locus_tag="QBH27_004245"
     CDS             complement(228..443)
                     /gene="bdm"
                     /locus_tag="QBH27_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDQ7016487.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            786..1217
                     /gene="osmC"
                     /locus_tag="QBH27_004246"
     CDS             786..1217
                     /gene="osmC"
                     /locus_tag="QBH27_004246"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDQ7016488.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(1407..3806)
                     /gene="dosP"
                     /locus_tag="QBH27_004247"
                     /gene_synonym="pdeO"
     CDS             complement(1407..3806)
                     /gene="dosP"
                     /locus_tag="QBH27_004247"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDQ7016489.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3831..3994)
                     /locus_tag="QBH27_004248"
                     /pseudo
     CDS             complement(3831..3994)
                     /locus_tag="QBH27_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(4372..5691)
                     /locus_tag="QBH27_004249"
     CDS             complement(4372..5691)
                     /locus_tag="QBH27_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDQ7016490.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNNSNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5822..7357)
                     /gene="gadC"
                     /locus_tag="QBH27_004250"
     CDS             complement(5822..7357)
                     /gene="gadC"
                     /locus_tag="QBH27_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDQ7016491.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         1932 a         1791 c         1836 g         1948 t
ORIGIN      
        1 ggggcgtttt ctgccggtgg gattatttac cacgctggat ttgtcacctt cggtaactat
       61 tttgcgctgg ttagaaattt tatggtccag tccaagaata tgacgtgcct gacggttcga
      121 tttcatttta actcctcaat cctgttgcta gttttaagga caacatcgcc gtagcgaaga
      181 aacacgtgct aaacccctaa attaggttgc cgatcaagca tagcacctta aagcgtaggg
      241 tgctgaccac tgaccacata attgatcgtt tgctggtaga tatcactgag gatgtcgtta
      301 tcagaagctt ccacccattt ggtcagctcc atgagaatgt catcttcagt gacaacaccg
      361 tgctctgccc gaaggccttg ctcaatggca ttaaccagag cgggttctgc tgttgaattt
      421 tctgcctgat aataagtaaa catagtgatt ctccgtgtct gtgtatttat ggtgtctgct
      481 acggatcgca gatttataaa gcacattcag catggcaaat atttgctgct tcgttgttaa
      541 gattagtcct ggttgatgat tttatatttt aactccatga tattgatagg tattattatt
      601 gtgatgagtt gattgatatt gatgcaatat ctcttgggtt atatatttcc ggttatctat
      661 tgtggaaatt taatttaagt gcagaaatta tattttcgcc ggattttatt cggattatcc
      721 tgcttatcct cgtgctgttt ctcacgtagt ctataatttc cttttaagcc cacaggagag
      781 caacaatgac aatccataag aaaggtcagg cacattggga aggcgatatc aaacgcggga
      841 agggaacagt atctactgag agcggcgtgc tgaaccaaca gccgtatgga tttaacacgc
      901 gttttgaagg cgaaaaagga accaaccctg aagaactgat tggcgcagcg catgccgcat
      961 gtttctcaat ggcgctttca ttaatgctgg gggaagcggg attcacgcca acatcgattg
     1021 ataccaccgc cgatgtctcg ctggataaag tggatgccgg ttttgcgatt actaaaatcg
     1081 cactgaagag cgaagttgcg gtgccgggta ttgatgcctc cacctttgac ggcattatcc
     1141 agaaagcaaa agcaggatgc ccggtttctc aggtactgaa agcggaaatt acgctggatt
     1201 accagttgaa atcgtaaagc gttgccggat gacgcgtcag gcgcgtgaat gcctgatgcg
     1261 tcagcacaat cacatcaggc tgagaaggga ttgttctgac atggctatgc gttgtcgttg
     1321 tccaactaaa cgtagcggaa atcacagcat ttaagtgaca gtgtcacgtt aaataaaaac
     1381 ccgcgagtgc gggcgagagg aatttatcag attttcagcg gtaacacgct gctcatccag
     1441 cctggaattt cttcggcggg cagggggcgg gaaaagaaat atccctgaat aacgcgacag
     1501 tggatcttgc gtagcatctc aaattgctct ttggtttcga cgccttccgc cacgacggtt
     1561 aaattgaggc tttgcccaat gctggtaatg gcttcgagta aggcaaggat gcgtttttca
     1621 gtcagacaac gatcgacaaa acttttgtca attttgattt ccgttaccgg aagactgact
     1681 aagcgggata atccggaaaa gcccgtacca aaatcatcta ccgataaacc gatgcccata
     1741 tcgcgcagga tctgaatgcg cttaaagatt tcggtatcgt gttccatcat catgctttcc
     1801 gtgatttcca ccgtcagctg gtggccgtca ataccccagg cgtgcattgc atcagatacc
     1861 tgattaggca gctgattact gcgaaagtgc agcgcagaca agttaacgga taacgccggg
     1921 atatgaatat tctggctacg ccattctgct aactgacggc aagcttccgc gatgacccag
     1981 cgcccaatat tttcgatttc accaatctct tctgcgagag gaataaaccg tgaagggggc
     2041 acatgaccat gcaggggatc gtaccagcga gcaagggctt cgatgccgta cagttcaccc
     2101 gtttctgcga agatttgcgg ctgataaacc agtttaagtt ggttattgct aatcgcttct
     2161 tttagcgctg cgcctaaaac caaacgctct tttaccattt cgttcatcgc cgggctgaag
     2221 aactgccagc cgttaccgcc atttttgcga ataaaatcca ttgcattgtg agcagtggag
     2281 agcaggtaat cgcggttttt acccacgtcg tagctgatgc caatgctcaa ggtgagcggg
     2341 aatggtttat cgtcaatcat tatcggcttg ctgaccacat tccgtagctc atcggcgatc
     2401 tgggtaatgt tactgacatc gttttccagg ctcacgagga caaaagacgc accttcaata
     2461 cgacagagat actgatccgg tttgagtttt tcacgaaagc gattgaccac ttccagcaat
     2521 gcctgatcgg cccacgcata gccaaggcta tcaatcacat cctgaatatg gtcaacaccg
     2581 atgagataca ccaccggaga gacggctttg tcgaccaggt catcgaggta attgtgcagg
     2641 ttattgcgat ttggcagacc ggtcatcgga tcaaattgga tgagttgttc aatatgctga
     2701 cggctttttt cctgttccag cgccagcgcg gccatatgct ggctgatatc tgccacgcgt
     2761 tcgataaagg cgctggtttc tgctcctgac gaggttttaa tttgcaggat ccccgcaggc
     2821 gcgccatcgc gctgacgaat ggtcgctgac cagctttgcg cattttgaac ttctgcaccg
     2881 tgcgaagatg acgcccagtg tatcggcatc ccgttgcgca gcgcgaacag agaaacatgc
     2941 gattcgttga gaacagattc gatattacga caaatgattt cccccatttc atgaaatggt
     3001 gggctgctgc acatggcggc gagaatattg ccttcaagct ggcggatttg cctttcttcg
     3061 gtgatatccg agaatgtcat taccaggttc tgcagatgcg cgagcacgtc ataaaccggg
     3121 ctgatagagg ctttaatcca gattttttca ccggtgcgcg tcaacagcag aaattcgtcc
     3181 tgatcgcggg cggttttcca tagcaactgc tgtaaacgaa tacggttatc agcagggaat
     3241 tctggaatgt tcaggagtgt atcgggctgc ataccgctgg cttcgttaat gcagtaacca
     3301 aacatttcgg taaatgcgcg attgcactgc acaatatggc gttccggatc gaggacaatc
     3361 accggtcggt cgagatggtc aacggcaata atcaattgtc gggtctgttc tttttgcgcc
     3421 atttctacgc tggcatcccg taccagcgcc aggtaataaa ctttcccctc ggcgctcact
     3481 ttcgatagcg caaaacgggt ccagatttta ctgccgtctt ttttctccag ctgcagctcc
     3541 cgactcatcc cctcgacgcg cgctttacca ccttcacggt tgtgacgaat gtattgagga
     3601 tgcgcaggac gcaaatcccg cggaatcagc atatcaatgt tattgccaat gacttcttca
     3661 cgtttgtacc cccagagctt ctctgcggcg gggttgaaaa acatcacttc atcattttcg
     3721 ttaattaaca ccgcgcccat catattttgc tcaagggcgg ggaaaaaaat gccatcggcg
     3781 gcattatccg catcggttag cttcatgatt acctctgcat cctggcgcat ctaaagactg
     3841 gctttacaga gttcaacacg gtttctacct cgtcttttgc gatatacaga gcttcatcgg
     3901 ctatttgaat gaggcgctca tagtcaggat gaccattaaa catggcggca ccgatggaaa
     3961 gtgagagggc aatatcctcg ccgtttgcgg ctttaaaata atgtcgacct ttatggttaa
     4021 accgcaggcc aaaatcaacc aaacacaacg tgcgggattg gctaactgca ccatattctc
     4081 ctgaaatatg aagatatact gaaaagaaat aagcgactta ggacagtttc aatctacgct
     4141 actgttcttc agaagagtat agcccatcgt aattattttt cggtgacagc gaatatcgta
     4201 tgttttttca tattcataca tttttattag ggggattatg gctgtttaac taagtgtggt
     4261 taattgcgtg gttaatttga cgtatttaag catgattgct agtgggatag ttcaagaggg
     4321 taacaagcca gtgggtaaag caccggcttg ttacaaagta agaatgggag tttaactgcc
     4381 ccaacgactt tgcagatagc tgaccgcttg ttgagtctgc ggtttattca gatagtcctc
     4441 acggaacaag attgtgccgc taatttccgg cacagcatcg ttaagatcga gctgcttttt
     4501 cagttccggt acgccgccgt taatcatcca gtctggctct atctttgacg gttctcctac
     4561 tttatagaag gcgataccga tatacaggcg ggtcctggtc ggtttaacga catccgccca
     4621 ccattttgcc aacacgtcat aacgcgcggc actccgtgag aatggccagt aaatttgggg
     4681 agcaatgtaa tccagcaacc cttgttccac ccatcgacgg gtatcagcgt aggattcgtc
     4741 ataggctgcc gcgcctcggg tatcggaacc gagcggatcg tgtgatcggt tacgccacac
     4801 gcctgccggg ctaacgccaa attcgacttc cggcttaatg cttttaatgg tgtgcgaaac
     4861 cttcgcaatt aactgctggg tattgttgcg ccgccagtct gcttttgacg caaatgcgcc
     4921 tccgtatttg cggtacgttt cgttatcatt tagccgtgaa cctggtgact cagtatagaa
     4981 atagtcatca aactgcacgc catctaccgg atagcgggaa accacttccg caactattga
     5041 tgtgatccag tcctgaacct cagggatgcc cgggtcgagg acaaagcgat cgccggacgt
     5101 tctgatccag tcgcggtgtt gcacatagac gctggccggt tgttgagaca gggtgctatt
     5161 cagttccctg atagtaccgg gcttcgtatt aaccgatacg cgataggggt taaaccaggc
     5221 gtgtactttc atcccacgct tgtgggcttc gtcgagcata aattgcagcg gatcgtaacc
     5281 cggattttca ccaatcttac cggtcataag atcggaccac ggcaaaattt tcgatggcca
     5341 cagggcggta ccatccggct tgacctgaaa aaagaccgtg tttatgccga gacgttgcag
     5401 atgatccagt ttgtcgatca tcgcctgttg ttgtacacgt gcccggctgg tggggttact
     5461 attgttaacc gaggaaaccg gtggccagtc gaggcgagac accgtggcca gccagatgcc
     5521 acgcatcggt tgtgacgatt gttgcgtcgt ggctggtggc tttgagcccg ctggtggtgt
     5581 caccatggac tctggaggcg tgcttttaca actacacagt aaaagtgcca gtgcaaccag
     5641 tatcgctggt cttctaatcg ctaatttctc gtttcgggag cagatatcca tatgtgctgg
     5701 tttccggtaa acagatgtgc gctcattctc gtacttatcc cggttaagtc aatacgacaa
     5761 caagcacgaa aaagggagcg atgaattatc gctcccttgt cttataagca ttcagacatg
     5821 gttagtgttt cttgtcattc atcacaatat agtgtggtga acgtgcacgc gggtgcagga
     5881 agaagtgacc ttttggtgcg ttctgactgt tgattggctc cagagtgacg ccggtattcg
     5941 ctttgctttt acgatcatga acagcataga gaataaaggg cagggcaagt accaccagga
     6001 aactaacgac cagtaattca acatacatat cggtagagtc accctggatg ttatccggcg
     6061 gcaggaagga aacaataaac gccataattg aagtcagcag accgacaata gccacgacca
     6121 gtttcacccc tttaccacca gggatattaa atgtgcgttt taagtcagga tgtttaagca
     6181 ccaacacaat gtagccaata aacagcatga aataagcaca cagataaatc accaccgtca
     6241 gcgccagtgc gatcaggaag gacatgttgt taccgccacc ggtattggtg aggatgatca
     6301 acgcgataga agtaatcacc aactgcgaaa tgaccagcgt taccggtacg ccatttttgt
     6361 tcattttagc gaatgccgct ggcagcaggt ttttctgcgc tgtgacatac atcccgcgag
     6421 aaggaccaac aatccaggaa gcgatttccg ccagaacacc cagcaacagc agtgcggaga
     6481 tcacgcgaac cgtccactca atttccggtg ccacatgtga catcagaacg gtaaaggttt
     6541 gcattacccc tgcggagagg ttgatttcat tacccggaat gaccatcgca atagacaaac
     6601 cgccaacaga gcttaggcag attgccgcca ccatcagcag taacatagcc agtggatagt
     6661 cgcgcccggg gttgctcatt tcattgacgt gggttgcgga agcttcaacg cccatataac
     6721 tcaaaatgaa ggcaacaaat acaaccaggg tgcccacttt agagaagtca gggaagaagg
     6781 tcttcgcatc catttcgata gcaacggggg caccggagtg cagataaata gccgctaatg
     6841 cgatcaaaat aaatgcaggt aacaggatac cggcgaagaa gccaacttta gcaattcgcg
     6901 ccgtgtattt cgtgccacca aactgcgtta atgccagcgc ccaaagaatg atgagtgctg
     6961 caatagtttt ggtaatgggg tcttcattca gcgctggcca tttcaggatg taggagagtg
     7021 cccctaacac gaaatagagc atcggaataa aaccaatggc gatttgcaga tagccaaatg
     7081 agatcgctgc aaatccccat ctcggcccca gagtatttga tacccaggcg aagacgccac
     7141 cttcttccca gccgtcgacg gtagccattt ccgcagcaca aagtcccacg ggaataaacc
     7201 ataaaatccc gcctaatagc aggaagaaga ctaatgaaaa gcccgatgtt gcgaaggtag
     7261 ggtattcata aacagccatt accatcgatg ccgttatggc aaaaaatccg agtaatgtga
     7321 gctgcttagc tttacctgtc tgtactgatg tagccatatt atccccctaa aacggtattc
     7381 ctgtcggaac cgcacctgtt ttgttctgaa gcgtattcag aacaatattt tccgttgcta
     7441 atgccagtga acagactttg gaaattgtcc cgaaacggct tcgtttcggg acaccgttac
     7501 cgttaaa
//
LOCUS       DAOFSV010000185         7326 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.185, whole genome shotgun sequence.
ACCESSION   DAOFSV010000185 DAOFSV010000000
VERSION     DAOFSV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.185"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            285..1400
                     /locus_tag="QBH27_004251"
     CDS             285..1400
                     /locus_tag="QBH27_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDQ7016492.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADQEGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1993..2844
                     /gene="hchA"
                     /locus_tag="QBH27_004252"
     CDS             1993..2844
                     /gene="hchA"
                     /locus_tag="QBH27_004252"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDQ7016493.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2952..4310)
                     /gene="hprS"
                     /locus_tag="QBH27_004253"
     CDS             complement(2952..4310)
                     /gene="hprS"
                     /locus_tag="QBH27_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDQ7016494.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4310..4981)
                     /gene="hprR"
                     /locus_tag="QBH27_004254"
     CDS             complement(4310..4981)
                     /gene="hprR"
                     /locus_tag="QBH27_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDQ7016495.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5114..5527
                     /gene="hiuH"
                     /locus_tag="QBH27_004255"
     CDS             5114..5527
                     /gene="hiuH"
                     /locus_tag="QBH27_004255"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDQ7016496.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5636..6640
                     /gene="msrP"
                     /locus_tag="QBH27_004256"
     CDS             5636..6640
                     /gene="msrP"
                     /locus_tag="QBH27_004256"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDQ7016497.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6641..7276
                     /gene="msrQ"
                     /locus_tag="QBH27_004257"
     CDS             6641..7276
                     /gene="msrQ"
                     /locus_tag="QBH27_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDQ7016498.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     TPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
BASE COUNT         2067 a         1696 c         1530 g         2033 t
ORIGIN      
        1 acaccagccg tttagcgcaa caatcattga tactccctat tttttcgatc aactcataac
       61 atattgatat atatccataa tattcgtgaa tatttatatt cagaattcat aactatgaat
      121 tatattaaat agattagaaa cattttgaaa tcttttaaat acatttgtta catgtaatct
      181 ttaaaataaa atgaacttca tagaatagta tccaaatgtg ctttttttgg ataacagcac
      241 ttattgatat attcatgaag attataatca caagggaata cataatgaaa agaaaagttc
      301 tggcaatgct ggtcccggcg ttattagttg ctggcgcagc aaatgcggct gaaatttata
      361 ataaaaatgg caataaagtt gatttttacg gcaagatggt tggcgaacgc atctggtcaa
      421 atactgatga taataacagc gaaaacgaag atacctccta tgctcgtttc ggtgttaaag
      481 gtgaaaccca aatcaccagc gaactgactg gtttcggtca gtttgaatac aacctcgacg
      541 ccagcaagcc tgaaggtgaa aaccaagaaa aaacccgttt aaccttcgcc ggtttgaaat
      601 ataacgagtt aggatcattt gactatggcc gtaactacgg tgttgcttat gatgccgcag
      661 cttataccga tatgttagtt gaatggggtg gcgattcctg ggcttccgct gacaacttca
      721 tgaacggtcg taccaacggt gttgcaacct accgtaactc tgatttcttt ggcctggttg
      781 atggtcttga ttttgcgatt cagtaccaag gcaaaaacag caaccgtagc actaagaaac
      841 aaaatggtga cggctatgcg ttgtctgtag actataacat caatggtttt ggtatcgttg
      901 gtgcatatag caaatctgat cgcaccaacg atcaagttgc cgatggaaac ggttcaaatg
      961 ctgaactgtg gtcattggct gcaaagtatg acgcgaacaa tgtctacgca gctgtaatgt
     1021 acggtgaaac tcgtaatatg actccgggtt ctattgatac aggtgttgcc gatcaagaag
     1081 gcaatacaat aatgcgtgat caacttatca atgaaaccca gaactttgaa gcggttgttc
     1141 agtaccaatt cgactttggt ctgcgtccgt ccttaggtta tgtgtactct aaaggtaaag
     1201 atatcaaagg tgtacctgga catagatatg ttgacgctga ccgtgtgaac tacattgaag
     1261 ttggtacctg gtactacttc aataagaaca tgaacgttta tactgcatac aaattcaaca
     1321 tgctggataa agatgacgcc gccattactg gcgcagcagc tgatgaccag tttgcagttg
     1381 gtatcgtcta ccagttctaa tcagccaacg cgccgaggcg tgttactgta aaacataaca
     1441 ccagcctgag ttcgtccctc atcgaactca ggctttttta ttggcatttc atctccatct
     1501 gcggcacacg caattgccaa agcatatcga tacagcacat taccccgccc ctcctggctc
     1561 aataaaatct accagcgtct cttccagcca acacccaaat gcatttcttt atgaattaat
     1621 ccgcaacaaa accgcttccc atatagaaat atttgcaaaa atagtgcatg aaaaaaactt
     1681 tttccctacc aatccgggat ataaatattc atcagtacca caacattcca cagcactaaa
     1741 tctctcccca tcgcccccaa acctctgata atggtctaaa atcattgagg ccacttgcga
     1801 cgacgttact caataaggaa agtttggcgt gaataatatt caacaccagt gcagcatcat
     1861 cattacatac ttatgcccgc caactaccgc tttcgatata ttgactcaat ttttcagcct
     1921 ggttaaaaat agcgaccagc tcagtcgcaa atatagtgac taccctaact aagcaacaat
     1981 aaggaataca ctatgactgt tcaaacaagt aaaaacccgc aggtcgatat tgctgaagat
     2041 aatgcgttct tcccttcaga atattcgctt agccaatata cgagtcccgt ctctgatctt
     2101 gatggcgtgg actatccaaa accgtatcgc ggtaaacata aaattctggt gatcgccgcg
     2161 gacgaacgtt atttgccgac cgataacgga aaactgttct cgaccggtaa ccatccgatt
     2221 gaaacgttgc tgccgttgta tcatctccat gctgcaggtt tcgaattcga agtggcgacc
     2281 atttccggtc tgatgaccaa gtttgaatac tgggctatgc cgcacaaaga tgaaaaagta
     2341 atgccattct ttgaacagca taaatcgtta ttccgtaatc cgaagaaact cgcggatgtt
     2401 gtcgccagcc tcaacgctga tagcgaatat gcagcaatct ttgttcctgg tgggcatggc
     2461 gcacttattg gtttaccaga aagccaggac gtggctgccg ctttacagtg ggcaatcaaa
     2521 aatgaccgtt ttgttatctc cctttgccac ggtccggcag cttttctggc acttcgccac
     2581 agcgataacc cactgaatgg ttattctatt tgcgcattcc cagacgccgc agacaaacaa
     2641 acgccagata ttggctatat gccgggtcat ctcacctggt acttcggcga agagttgaag
     2701 aaaatgggca tgaacatcat taatgacgac atcaccgggc gagtacataa ggaccgtaaa
     2761 cttctcaccg gcgacagtcc ttttgcagcg aatgcgttgg gtaaactggc ggcgcaggaa
     2821 atgctggcag cttacgcgag ttaatccctg ttacattgta atgtaagcgc ataatcaatc
     2881 gcagccacgt taatactcac gacgtggctg cgttttttca cggttaactt atggcgtact
     2941 gaagcccgat gttaatttct ttgcggtaac gtaattcgga acacgttatg cttactgaga
     3001 tagtgatacg tagcacttcc tccatgtaat tcggcaatcg ctttgactaa agaaaggcca
     3061 agtccctgac ctacggaatg acgcgaatta tctccccgcc aaaatctacg gaagagtttt
     3121 tcaggctcat taattttcgt tccaggactg gcgatatcaa tattaagaga accgttggca
     3181 tcaagaaaac tggttatatg aatacgcgat ttttctggtg aatatcgaat ggcgttaaca
     3241 ataagattcg ataacattcg ttgcagtaaa attttatccg caaagatttg ttgattgcac
     3301 ttgaccttaa agcaaatctc tttctcgtct gaaagatatt caagatagtc caaaagattt
     3361 tcaacttcct tattgagaga aagcgaatcc agtttcacca aaacattgtt tttgtctgcc
     3421 ctggcaagaa acagtatatt ctctgttaac cgcgaaatat tttccagctc ttcgatgttt
     3481 ccggcaattg ttttttgata ttcagcgata cttctggttt gactgagcgt aacctgattc
     3541 tgacccagta atgcattaat tggcgttcta agttcatgag cgagatcgtc agcaaattga
     3601 ctaaggcgct caaaatcttt gactaaagca tgatgcattt tattcaacgc ctgcccaaga
     3661 ggttttagtt ctctcggtaa tgcgttaacc tcaaccggca ctcggctatc gttataattc
     3721 agcgcttccg ttacgccact caactttttg atctctcgca accccgttct gattaacaac
     3781 ggacttaata atgagcacaa tataatgacg acaatgcaaa ttataatgct attaatttta
     3841 tactgttcaa gcatattatg tctggctgaa gccaatttag ccacagtaac cgttaatgat
     3901 ggcgaaactt catcaatgtt aattcgtaaa gcatctatct ctgtatcatt aataatgctt
     3961 ctgtaaatgc ctgctgcgct gattgtctca ctagcaggta tgttatttaa catgtcatca
     4021 ctgacatttg tccggttaac aattttattg atgccatcac catgaatgat caagatatcc
     4081 tgactaacat ccatcatccg gttaaaatag acaggtaatg tatctggatt taccccatca
     4141 attaacaact gcttgatctg cgctgtccgg ttaatgagtg ttgtatcatc gcgccatttc
     4201 aactcacttg ccaggccatt atagagagtc catacaattc cagcgccagc aacagacagc
     4261 agaaatataa aaagcaaggt taaacggacg gttatagata atcttttcat tattttttta
     4321 ccgctacgaa tgaatagccc atcccccgga ttgtggcgat tagcttttca ggaaaaggat
     4381 catcaacttt tgcgcggagc ctgcgaatgg cgacatccac cgtattggta tcactatcaa
     4441 agttgattcc ccaaatttca ctcgcaataa ccgttctggg tataatctcg ccagctctgg
     4501 aggccagtag ccaaagtaac tgaaactcct tgcgcgtcag tgtaatactg atattgtccc
     4561 tgctcacact ttgactgaca gagtccattt ttaagccact gatttctaat gttgaattca
     4621 aagcgtgatg ttgccttaat tgtgcccgaa cccttgccag caactcagaa aatgaaaaag
     4681 gttttaccag ataatcattt gctccactgt ccagccctct gactctgtca tcgacagaat
     4741 cccttgctgt aaggcaaata acaggagttt gctttgccgt tcttaacgtt tgtaagatct
     4801 gccagccatc catacccgga agcataatat ccagaatgat caatgcataa tcatccttca
     4861 gcgcaagata aagcccatct ctgccatcag aaacggcatc aatgacataa cccgcttcgg
     4921 aaagcccctg cgttacccat tcctgggtcc tttgattatc ttcaataagt agaatcttca
     4981 tgaaaataat atgccataaa tcatatgtta tagaaacaac ctggttcatt acaaaattgt
     5041 aatgctgctg taaggttacc ctggacgttt tttcgctatc ctcaaaactc attcacatga
     5101 caaggatata aacatgttaa agcgttattt agtactctcg gtagcaacgg cagcattttc
     5161 attaccttct ttggtttatg ccgcacaaca aaacattctt agcgtgcaca ttttgaacca
     5221 gcaaacagga aaacccgctg ccgacgtgac agtcactctt gaaaagaaag cggataacgg
     5281 ctggttacaa cttaataccg ccaaaacaga taaggatgga cgaattaagg cactgtggcc
     5341 cgagcaaact gcgactactg gcgattaccg tgtcgtattt aaaaccgggg actatttcaa
     5401 gaaacaaaat cttgaaagtt tcttccctga gatccccgtt gaatttcata ttaataaagt
     5461 gaacgagcat tatcatgtgc ctttgttact cagccaatat gggtattcaa cctatcgtgg
     5521 cagttaatgc gtaaatatgt aaaagctgat ctctgctact gttagggagg tgactccctg
     5581 acagattaac agtaaacggc tcttgttggc taacgacaaa aaagtgtgat ggcttatgaa
     5641 aaagaatcaa tttttaaaag aatcagatgt tacggccgag tcggtattct ttatgaagcg
     5701 tcggcaggtg ttaaaagcac tgggcatcag cgcagctgca ttttctttgc ctcacgctgc
     5761 gcatgccgat ctgcttagct ggtttaaagg gaacgatcgt ccgcccgccc ccgccggaaa
     5821 accgctggag ttcagcaagc ctgccgcctg gcaaaataac ctgccactga cgccagcaga
     5881 taaagtctcc ggttataaca acttctatga attcgggctg gataaagccg atcccgctgc
     5941 taatgctggt agcctgaaaa ccgatccatg gacactgaaa atcagcggcg aagtggcaaa
     6001 accattgacc ctcgatcatg atgatttaac ccgtcgcttc ccgctggaag agcgtattta
     6061 tcgtatgcgc tgcgtggaag cgtggtcgat ggtggtgccg tggattggtt ttccgctaca
     6121 caaattgctg gcgcttgccg aacccaccag caatgcgaaa tatgtcgctt tcgaaacgat
     6181 ttatgcgccg gaacagatgc caggccagca ggaccgcttt atcggcggcg ggctgaaata
     6241 tccttatgtc gaaggattgc gtctcgacga agcaatgcat ccgctcacac tgatgaccgt
     6301 aggtgtttat ggcaaggcgt taccaccgca aaatggcgcg ccggtgcgac tgattgtgcc
     6361 gtggaaatat ggctttaaag ggattaaatc gatcgtcagt attaagctga cccgcgagcg
     6421 tccgccaacc acctggaatc tggcagcgcc tgacgaatac ggtttttacg ccaacgttaa
     6481 tccgcatgtt gatcacccgc gctggtcaca ggctaccgaa cgatttattg gttcaggcgg
     6541 cattctcgat gttcagcgcc agccaacact actgtttaat ggttacgccg accaggtggc
     6601 atcgctgtat cgtggcctgg atttgcggga gaatttctaa atgcgcctga cagcaaaaca
     6661 ggtgatatgg ctgaaagttt gcctgcatct tgccggattg ttgccgtttc tctggctggt
     6721 ctgggcgatc aatcacggtg gactaggtgc cgatccggtg aaagatattc agcattttac
     6781 cggtcgcact gcactgaaat ttttgcttgc ggccttgtta atcacacctc tggcacgcta
     6841 cgcaaaacag ccgttattga tacgcactcg ccgcctgtta ggcttatggt gctttgcctg
     6901 ggcgacactg catttaacca gttacgcatt gctggagttg ggcgtgaata atctggcgtt
     6961 attaggtaag gagttaatta cccggcctta tttaacgtta ggaattatca gctgggttat
     7021 tttgcttgct ttagcgttca cttcaaccca gtcgatgcag cgaaaactcg gcaaacactg
     7081 gcaacagttg cataacttcg tctatcttgt cgcgatcctg accccaatac attatctgtg
     7141 gtcagtgaag attatttcac cgcagccact catctacgct gggctggctg tactgctttt
     7201 agccttacgg tataagaagt tgctttccct tttcaaccgg ttacgcaagc aggcccacaa
     7261 caaattatct ctataacttc atattgccga caaacttcgc gttatcccgg ccggatgcgg
     7321 cgcgag
//
LOCUS       DAOFSV010000186         7325 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.186, whole genome shotgun sequence.
ACCESSION   DAOFSV010000186 DAOFSV010000000
VERSION     DAOFSV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.186"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            22..1044
                     /locus_tag="QBH27_004258"
     CDS             22..1044
                     /locus_tag="QBH27_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016499.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYETIMENNTNKESLCLQSEKGGKTSYKDITK
                     IQLATTEKQKLLASLEVVNSMSSYVELLTVGMDVEDSKQIILLNQALDHANNAQKLLG
                     LSEGEELTKSVVAVKDLVQYFNGLYKEKIKSENVGKTVGSDWPRLQRNLQDLLDDIAT
                     KQQQMDRNNYANISNNFYYYNKAQANTKNIEFKTLKSRQEFLSAVINLNESRLRSSDP
                     AAPAIQAINAFMDKGNHLQDLYSNKSLSKADLERRNEILRSQVISGLQASEELAKLLI
                     ASGVML"
     gene            1054..1362
                     /locus_tag="QBH27_004259"
     CDS             1054..1362
                     /locus_tag="QBH27_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016500.1"
                     /translation="MNDVQNRRILALLNMLYYALGNRTTCDICHKYFILVLSVMNNLT
                     INYGSQTSPELTGISYEDANDLITELILFIEDVNADNDMEYPAHLNKIQALLNFAVAN
                     "
     gene            complement(1419..2009)
                     /gene="cobO"
                     /locus_tag="QBH27_004260"
     CDS             complement(1419..2009)
                     /gene="cobO"
                     /locus_tag="QBH27_004260"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDQ7016501.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(2006..2764)
                     /locus_tag="QBH27_004261"
     CDS             complement(2006..2764)
                     /locus_tag="QBH27_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDQ7016502.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2984..4033
                     /gene="sohB"
                     /locus_tag="QBH27_004262"
     CDS             2984..4033
                     /gene="sohB"
                     /locus_tag="QBH27_004262"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDQ7016503.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(4069..4320)
                     /locus_tag="QBH27_004263"
     CDS             complement(4069..4320)
                     /locus_tag="QBH27_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDQ7016504.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4700..7297
                     /gene="topA"
                     /locus_tag="QBH27_004264"
     CDS             4700..7297
                     /gene="topA"
                     /locus_tag="QBH27_004264"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDQ7016505.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1986 a         1640 c         1879 g         1820 t
ORIGIN      
        1 ctcaacgtca agaggtgggt tatgattaga tatagtggaa tatttctatc gctgttaatg
       61 ctttcaggtt gtgcaaccga agcattgaag aatgaacaag ctgaaactgt tattactgcg
      121 ggtaacaagg cggttgcggc gaatgcaagt ttctttaaac aatactatgc ttctcgtgaa
      181 aactttcttg ttaattttta tgcgactaat ccggattgtg cattgcgacc tgattacgaa
      241 acgattatgg aaaataatac taataaagag tccttatgtt tgcaatcaga gaaagggggc
      301 aaaacatcgt ataaagacat tactaaaatt caattggcaa caacagaaaa acaaaaacta
      361 ctggcttcac ttgaggtcgt taacagtatg agttcctatg tggagttgtt gacggtgggt
      421 atggatgttg aggatagtaa acaaattata ttacttaatc aggcgctcga tcacgcaaac
      481 aacgcccaaa aactacttgg cctttcagaa ggtgaagaat tgactaagtc agttgtggct
      541 gttaaagatt tagttcaata tttcaatgga ttatacaaag agaagattaa atcagaaaat
      601 gttggtaaaa cggtaggtag tgactggcct cgacttcaaa gaaatttaca agatttactc
      661 gatgatatag caacgaaaca gcagcaaatg gatcggaata attatgcgaa catcagtaat
      721 aatttttatt actacaataa ggcccaggca aatactaaaa atatagagtt taagacatta
      781 aagagcaggc aggaattttt gagtgcagta attaatctta atgaaagcag attgcgttca
      841 tctgatccgg cggctccagc aatacaagct attaatgcct tcatggataa aggaaatcat
      901 ctacaagatt tatactctaa taaatctctt tcaaaagcgg atttagagcg tagaaatgag
      961 atccttcgtt ctcaagttat tagtggtcta caggcgtcag aggagctggc aaaacttctt
     1021 attgccagcg gagttatgtt ataggaggtc attatgaacg atgtgcaaaa tcgacgaata
     1081 ctggcattgc tcaatatgct gtactatgcc ttaggaaacc gaacaacctg cgatatatgt
     1141 cacaaatatt ttattctggt tttgtcagta atgaataatc tgacaattaa ttatggctcg
     1201 caaacgtcac cagaactgac gggtattagt tatgaagatg ctaatgattt gatcactgaa
     1261 ttaatactgt ttattgaaga cgtcaatgcg gataatgata tggaatatcc cgcacacttg
     1321 aataaaattc aagctcttct taattttgcc gtggctaatt aagccattca gtatctaaaa
     1381 ttagcacctc aagaggcgaa cttttgaggt gctttcactt agtaatcaat ccctatctgc
     1441 gcttttacac cggcatcaaa cgcatgtttg atggggcgca gctcacttac cgtatccgcc
     1501 agttcaagaa tatcccgatg acagcctcga cccgtgataa tcaccgtctg ttgatgtgga
     1561 cgttcattta acgcctgaat cacgtcttcc agtggcaaat agtcatacgc taccatatat
     1621 gtcagttcat cgagcaaaac catatccagt gaggaatcag ccagcatccg ctttgcatgt
     1681 tgccagactt cgcggcaggc ggcggtatcg gactctcggt tttgtgtatc ccaggtaaag
     1741 cccgttgcca ttacctgaaa ctcaacgccg tgtggctcca gcagatttcg ttcaccatta
     1801 ggccaggtgc ctttaataaa ctgcacgacg cccacttttt ttccgtgacc aactgcgcgt
     1861 gttgccgtac caaatgccgc ggtggttttg ccttttccat tgccggtaaa gacgatgata
     1921 ataccgcgtt catcctgggc ctgggccaca cgagcatcta ctttttcttt cactcgctgc
     1981 tgacgctgtt ggtagcgttc atcactcatt gggaaattcc tggtttacgg cccggttggg
     2041 cgtcaaaggt catgccggtt ttacggcggc tgtcatcgcc catcagccag aggtagagcg
     2101 gcatgatatc agcgggggtt ttaagtttct gtggatcttc ggtcgggaag gcactggccc
     2161 gcattgcggt gcgcgtaccg cctgggttaa tgcagttgac acgcaggcgc tgctgatatt
     2221 catcggccag tacctgcatc atcccttcgg tggcaaattt cgacgctgca tatgcgcccc
     2281 agtttgcacg cccctgacgt ccaacgcttg atgaggtaaa gaccagtgaa ccggcgtcgg
     2341 atttgagtaa taaaggaagc agtgcctggg tgagcataaa ggtggcatta acgttgacct
     2401 gcatgacgtc ctgccagact tgcggatctt gttcgctcat tgggcaaaca tcgccgagca
     2461 atccggcatt atgcaaaaca ccatccagac gcggataatt aacggcaatg cgctgtgcca
     2521 gttgttggca atcttcggac gtgcaggtta gcaaatcgag aataaaccac tgtggctgac
     2581 gtccagtttc ttcgtttatg tggctggcta cctgacgtaa tttttcttca ttacggccca
     2641 aaagaatcac tgtcgcacca tagcgtgcat acgtcatcgc tgcttcacga ccaattccat
     2701 cgctggctcc cgtcaccaga ataatgcgat cattgagtaa atcttgtttt ggctggtaat
     2761 gcatggctac tcctcaacga cgttgtctgt cgcgctgcca gtgtacttta tgactttggg
     2821 gctttatgcc tgaaacagaa gggcatttca atcagcctgg ggcgataaca ccgcaaaatg
     2881 gatactttgt catactttcg ctgcaataac atctctgcga gacggctaaa catgcctgtt
     2941 gtaaactgtg agccaaagcg ttgtttaacc aaggtgggga ctcgtggaat tgttgtctga
     3001 atatggtttg tttttggcga aaatcgttac cgttgtgcta gcgattgcgg cgattgccgc
     3061 cattattgtc aatgttgctc aacgtaataa acgccagcgt ggcgagttac gagtcaacaa
     3121 tctcagcgaa cagtataagg agatgaaaga agaactggcc gcggcgctga tggacactca
     3181 tcagcaaaaa cagtggcaca aagcgcagaa gaaaaagcac aagcaagaag cgaaagccgc
     3241 aaaagcgaaa gctaaactgg gcgaggtggt aactgacagt aaaccccgcg tctgggtgct
     3301 ggattttaaa ggcagcatgg acgcccatga agtgaactct ctacgtgaag agataacggc
     3361 ggtactcgca gcattcaaac cgcaggatca ggttgtgctc cgtctggaaa gcccaggtgg
     3421 catggtgcat ggttacgggt tggcggcttc gcagttgcag cgtctgcgtg ataaaaacat
     3481 tcctttaact gttacggtag acaaagtcgc tgccagcggc ggttacatga tggcctgtgt
     3541 ggcggacaaa attgtttccg caccgtttgc tattgtgggt tccattgggg tggtggcgca
     3601 aatgcccaac tttaaccgct tcctgaaaag caaagatatt gatatcgaac tgcacaccgc
     3661 cgggcagtat aagcgcacgc tgacgttgct gggtgaaaat accgaagaag ggcgggagaa
     3721 attccgcgaa gagttgaacg aaacgcatca gttatttaaa gattttgtga agcgtatgcg
     3781 tccgtctctg gatattgaac aggtggcaac gggtgaacac tggtacggac aacaggcagt
     3841 agagaaaggc ctggttgatg aaatcaacac cagtgatgaa gttattctta gcctgatgga
     3901 aggccgggaa gtggtcaatg tacgctatat gcagcgtaaa cgactcattg accgattcac
     3961 cggcagcgcg gcagagagcg ccgatcgatt gttgttacgc tggtggcagc gtgggcaaaa
     4021 gccgttgatg taaaagacaa acgcgaggat aagacctcgc gttttgcttt aatcaaccag
     4081 gtgatatttt tctgaaagca catgggccag gtgtttgaac atattaaaca ccgcggtgct
     4141 tttggcagtt ggtaatcctt gttcatctaa aaagtagtcg ccggtaaata ccagcacgcc
     4201 attacgctgc gtgacgccgg tggcttcaat ccctgcgagc gtatcctcat gctcacgaat
     4261 gattttgttg gcctctttca acagcgtttc gcggtcgatg ggttgagtct ctttgttcat
     4321 tatttactcc ttaaacaagg acattagtct acgccaggca tggcttgcag acaaatatac
     4381 cacgctggtg gcaagagcgc cttactggca actttggatt ttgcatgcta ataaagttgc
     4441 gtatcggatt ttatcaggta cagtgtgacg ctttcgtcaa tctggcaata gatttgcttg
     4501 acattcgacc aaaattccgt cgtgctatag cgcctgtagg ccaagacctg ttaactcagt
     4561 cacctgaatt ttcgtgagca gagtcacgac aaggggttga tatccgcaga gagcgagtcc
     4621 atatcggtaa ctcgttgcca gtggaaggtt tatcaacgtg cgacgcattc ctggaagaat
     4681 caaattaggt aaggtgaata tgggtaaagc tcttgtcatc gttgagtccc cggcaaaagc
     4741 caaaacgatc aacaagtatc tgggtagtga ctacgtggtg aaatccagcg tcggtcacat
     4801 ccgcgatttg ccgaccagtg gctcaactgc caaaaagagt gccgactcta cctccaccaa
     4861 gacggctaaa aagcctaaaa aggatgaacg tggcgctctc gtcaaccgta tgggggttga
     4921 cccgtggcac aattgggagg cgcactatga agtgttgcct ggtaaagaga aggtcgtctc
     4981 tgaactgaaa caactggctg aaaaagccga ccacatctat ctcgcaaccg accttgaccg
     5041 cgaaggggaa gccattgcat ggcacctgcg ggaagtgatt ggtggtgatg atgcgcgcta
     5101 tagccgagtg gtgtttaacg aaattactaa aaacgcgatc cgccaggcat ttaacaaacc
     5161 gggtgagctg aatattgatc gtgttaatgc ccagcaggcg cgtcgcttta tggaccgcgt
     5221 ggtggggtat atggtttcgc cgcttctatg gaaaaagatc gctcgtggtc tgtctgccgg
     5281 tcgtgtgcag tcggtggcgg tccgcctggt ggtcgagcgt gagcgtgaaa ttaaagcgtt
     5341 cgtaccggaa gagttctggg aagtcgatgc cagcacgacc acgccatctg gtgaagcgtt
     5401 ggcgttgcag gtgactcatc agaacgacaa accgttccgt ccggtcaaca aagaacaaac
     5461 tcaggctgcg gtaagtctgc tggaaaaagc gcgctacagc gtgctggaac gtgaagacaa
     5521 accgacaacc agtaaacctg gcgctccttt tattacctct acgctgcaac aagctgccag
     5581 cacccgtctt ggttttggcg tgaaaaaaac catgatgatg gcgcagcgtt tgtatgaagc
     5641 aggctatatc acttacatgc gtaccgactc caccaacctg agtcaggacg cggtaaatat
     5701 ggttcgcggt tatatcagcg ataattttgg taagaaatat ctgccggaaa gtccgaatca
     5761 gtacgccagc aaagaaaact cacaggaagc gcacgaagcg attcgtcctt ctgacgtcaa
     5821 tgtgatggcg gaatcgttga aggatatgga agcagatgcg cagaaactgt accagttaat
     5881 ctggcgtcag ttcgtagcct gccagatgac cccagcgaaa tatgactcca cgacgctgac
     5941 cgttggtgcg ggcgatttcc gcctgaaagc acgcggtcgt attttgcgct ttgatggctg
     6001 gacgaaagtg atgcctgcac tgcgtaaagg cgatgaagat cgtatcttac cggcagtcga
     6061 taaaggcgat gctctgacgc tcgttgaact gacaccagcc cagcacttta ccaagcctcc
     6121 agcccgtttc agtgaagcat cgctggtcaa agagctggaa aaacgcggta tcggtcgtcc
     6181 ttctacctat gcgtcgatca tttcgaccat tcaggatcgt ggctatgtgc gagtagaaaa
     6241 tcgtcgtttc tatgcggaaa aaatgggcga aatcgtcacc gatcgcctgg aagagaattt
     6301 ccgcgagtta atgaactacg acttcaccgc gcagatggaa aacagcctcg accaggtggc
     6361 aaatcacgaa gcagagtgga aagctgtact ggatcacttc ttctcggatt tcacccagca
     6421 gttagataaa gctgaaaaag atccggaaga gggtggtatg cgcccgaacc agatggttct
     6481 gaccagcatt gactgcccga cttgtggtcg caaaatgggg attcgcacag cgagcaccgg
     6541 ggtattcctt ggctgttctg gctatgcgct gccgccgaaa gagcgttgca aaaccaccat
     6601 taacctggtg ccggaaaacg aagtgctgaa cgtgctggaa ggtgaagacg ctgaaaccaa
     6661 tgcgttgcgc gcaaaacgtc gttgcccgaa atgtggcacg gcgatggaca gctatctcat
     6721 cgatccgaaa cgtaagttgc atgtctgtgg taataaccca acctgcgacg gttacgagat
     6781 cgaagagggt gaattccgca ttaaaggtta tgacggcccg atcgttgagt gtgaaaaatg
     6841 tggctctgaa atgcacctga aaatggggcg tttcggtaaa tacatggcct gcaccaacga
     6901 agagtgtaaa aacacacgta agattttacg taacggcgaa gtggcaccac cgaaagaaga
     6961 tccggtgcca ttaccggagc tgccgtgcga aaaatcagat gcctatttcg tgttgcgtga
     7021 cggtgctgcc ggtgtgttcc tggcggccaa taccttcccg aaatcgcgtg aaacgcgtgc
     7081 gcctctggtg gaggagcttt atcgcttccg cgaccgtctg ccggaaaaac tgcgttatct
     7141 ggccgatgcg ccacagcagg atccggaagg taataagacc atggttcgct ttagccgtaa
     7201 aaccaaacag caatatgtct cttcggaaaa agacggaaag gcgactggtt ggtcagcatt
     7261 ttatgttgat ggcaaatggg ttgaagggaa aaaataacct ttaattccgt ctggttttta
     7321 taaac
//
LOCUS       DAOFSV010000187         7140 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.187, whole genome shotgun sequence.
ACCESSION   DAOFSV010000187 DAOFSV010000000
VERSION     DAOFSV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.187"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            15..866
                     /locus_tag="QBH27_004265"
     CDS             15..866
                     /locus_tag="QBH27_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDQ7016506.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKSHNGFPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVVKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            859..1230
                     /locus_tag="QBH27_004266"
     CDS             859..1230
                     /locus_tag="QBH27_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDQ7016507.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1227..1979
                     /gene="fliP"
                     /locus_tag="QBH27_004267"
     CDS             1227..1979
                     /gene="fliP"
                     /locus_tag="QBH27_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDQ7016508.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            1982..2254
                     /gene="fliQ"
                     /locus_tag="QBH27_004268"
     CDS             1982..2254
                     /gene="fliQ"
                     /locus_tag="QBH27_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HDQ7016509.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2256..3038
                     /gene="fliR"
                     /locus_tag="QBH27_004269"
     CDS             2256..3038
                     /gene="fliR"
                     /locus_tag="QBH27_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HDQ7016510.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHNLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3028..4167
                     /gene="flhB"
                     /locus_tag="QBH27_004270"
     CDS             3028..4167
                     /gene="flhB"
                     /locus_tag="QBH27_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDQ7016511.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLSLQALPFKLAINDGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4151..6244
                     /gene="flhA"
                     /locus_tag="QBH27_004271"
     CDS             4151..6244
                     /gene="flhA"
                     /locus_tag="QBH27_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDQ7016512.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(6464..6961)
                     /gene="rayT"
                     /locus_tag="QBH27_004272"
     CDS             complement(6464..6961)
                     /gene="rayT"
                     /locus_tag="QBH27_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDQ7016513.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTQDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAEDVTDFSAG
                     ERIIL"
BASE COUNT         1588 a         1887 c         1994 g         1671 t
ORIGIN      
        1 agagaaagta agccatgctg aagtacagta aaacgccagg catctttaaa ttagaaggta
       61 atcggcttgg acgcccctac catcgcctgc cgaccctgtt tacggggaat tttgatgtaa
      121 ttgattcgca tcttggcagc tactttctca aaaagcatcg cagcaatatc accctaaaaa
      181 agattggctg cgaaatggat attattaata aaaatgccga gctgatggtt tcccaggtcg
      241 ggcatctggc gtttgatatt gatcgctcct tgctgttaat gctgctgggc aatttttatg
      301 gtctggagtc gtctcttgaa gaagcgaaat cacacaatgg attcccgacc aaaacggaaa
      361 cacgtttaaa aaaccgcctc gcgctggata tctgcacgct gatttttaat ctgcaaacct
      421 ccggtattgc gctgaaatta aaactggaca gcagcacggt aattacccac tgggcgtatc
      481 aattaacctt tacccttgcg ggcgatgaag agagcagttt tcgtattttg ctcgacgacg
      541 cccacaccga ttttattttg aatctgatcc gccacagcga gcacagtaaa ccgcagcagg
      601 tggtgaaatc cgttaataaa ccggccctga ttaaagaaat catccgctcc ctgccgctga
      661 ccctgaacgt caaaatcgcc gagctatcga tgaatgtcgc ggatctggca cagattaaag
      721 ccggggatat tttacccata tcactgggag aaacgttccc ggtggcgatt gggcaatctg
      781 aattatttag cgccctgatt gtggaagaca aagacaaact gtttttgtcg gagctggcgg
      841 gaaaaaatga gaacaaccat gagtaagcaa gaagatattt tagccgggga ttttggcctc
      901 accgatgaag ttgcgcctgt cgccaaatcc gccgacgccg aaacgctggt cacccgcctg
      961 gaagaccgat tttccgactc gatgacgcta ctgaaacgca ttccggtgac cttaacgctg
     1021 gaagtatcgt cggtggaaat catgttagcc gacctgctta acatcgacga cgacacggtg
     1081 attgaactgg ataaactcgc cggggagccg ctggatatta aagtgaacaa tatcctgctg
     1141 ggcaaagcgg aagtggtggt ggtcaacgag aagtacggcc tgcgcgtgct ggaatttaac
     1201 acccgcgata tcaacgacct ggcgccatga aacgccgcac tcaactgacg ctgggcctgg
     1261 cgctgctggc gctcgccccg ctggcgctgg cccagggcgg cgatatcgca ctgcttaacg
     1321 tggtcaccca cggcaacact caggagtaca gcgtcaaaat tcaggtactg attctgatga
     1381 ccctcgtcgg tttgctgccg acgatggtgc tgatgatgac ctgtttcacg cgttttatta
     1441 tcgtgctgtc actgctgcgc caggcgctgg ggctgcaaca aacgccgcca aaccgcattc
     1501 tgattggtat tgcgctctca ctcaccctgc tggtaatgcg ccccatctgg ctcaatattt
     1561 acgaccacgc ggtagtgccg ttcgaaaacg atcagattac cctgaccgat gcgttaagca
     1621 ccgccgcgac gccgctaaaa cgctttatgc tggcgcagac cgacaaaaaa gcgatggcgc
     1681 aaatcatgac catcggcggc gcgaagggca atgccgccga ccaggatctc tccatcgtgg
     1741 tgcccgccta tgtactgagc gagctgaaaa ccgccttcca gattggcttt atgatttaca
     1801 tcccgtttct ggttatcgac ctgattgtcg ccagcgtatt gatggcgatg gggatgatga
     1861 tgctgtcgcc gctgattgtt tcgctaccct ttaaactgat gctgtttgtg ctgattgacg
     1921 gctggtcgct gaccatcggc acgctcacca ccagcattcg cgggctgggg ctgggctgat
     1981 tatgttgacc gtagacgtcg ccgccgacat tgtcgccagc ggcattaaag tagtgatttt
     2041 gctggtttcg gtactggtgg tgccaagcct gctggtcggc ctgctggtga gcgtgttcca
     2101 ggcggtgacg cagattaacg aacagaccct gagctttttg ccaaggctga ttgtcacgct
     2161 ggtggtgctg ggcgtgtgcg gcaaatggat gattatccag ttgcatgatc tctgcattca
     2221 cctgtttacc caggccgcgt tgctggttca ttgagatgcg cacaagcgac gttacgcaac
     2281 tgatggatct ggcgctgggg ctgtggtttc ccttcgtgcg cattatggcg tttctgcgct
     2341 atatgccggt gctggataac agcgccctga ccgcgcgcgt gcggattatt ttgtcgctgg
     2401 cgctggcgat catcatcacg ccgttgatcc cccatcccat tccccacaat ctgctatcgc
     2461 tcaacagcct gatcctgacg gtggagcaaa ttttatgggg catgttattc gggctgatgt
     2521 ttcagttttt gtttctggcg ctacaactgg ccgggcaaat tctctcgttc aatatgggga
     2581 tgagcatggc ggtgatgaac gacccgagca gcggcgcatc gaccaccgtg ctggcggagc
     2641 taatcaacgt ctacgcgatc ctgctgtttt ttgccatgga cgggcatctg ctgctggtaa
     2701 gcgtgttgta caagggcttc acctactggc cgattggcaa cgccctgcat ccgcaaaccc
     2761 tgcgtaccat cgcgctggcc tttagctggg tgctggcgtc ggcatcgctg ctggcgctgc
     2821 cgaccacttt tatcatgctg attgtgcagg gctgctttgg cttgctcaac cgtattgcgc
     2881 cgccgctgaa ccttttttct cttggctttc cgatcaacat gctggcgggg ctggtctgtt
     2941 ttgccaccct gctctacaac ctgccggatc actatctgca tctggcgaat tttgtcttac
     3001 aacaactcga cgcgctgaaa ggtcactatg gcggatagca gcagcgaaga aaaaacagag
     3061 aaaccctcgg cgcaaaagct gcgtaaagcg cgtgaagagg ggcaactgcc gcgctcgaag
     3121 gatatggggc tggcggcgag cctgtttgcg gcgtttgtgg tgatctccag cagttttccg
     3181 tggtatgccg attttgtgcg cgagagcttt atcagcgtgc atcagtatgc gcaggagatt
     3241 aataacccgc aggtgatcgg ccagtttctg cgccatcacc tgttaattct cggcaagttt
     3301 atcctcacct tgctgccgat gcctgctgcc gcgctgctct cttcgctggt gcccggcggc
     3361 tggctgtttc tgccaaaaaa aatcctcccg gatttcagca aaatcagtcc gttaaaaggc
     3421 attgggcggc tgttttccag tgaacatctg gcggaaacgg gaaagatgac ggtgaagtct
     3481 gtggtggtgc tggtgatgct gtgggtaagc ctgcgcaata actttgccgc ctttctcagc
     3541 ctgcaggcgc tgccgttcaa actggcgata aatgacggtt tatcgctcta cgccagcgtg
     3601 atgcgcaact ttgtcattct gtttatcttc ttcgcgctta tcgacgtgcc gctggcgaaa
     3661 gccctgttca ccaaagggct gaagatgacc aaacaggagc tgaaagaaga gtacaaaaat
     3721 caggaaggta agccggaagt gaaggcgcgt gtgcggcgac tgcaacggca actggcgatg
     3781 gggcaaatcc gcaaagtggt gccgaaagcc aacgtggtga tcaccaaccc gacccactac
     3841 gccgtggcgt tacagtatga ccagtcgcgc gccgccgcgc cgtttgtggt ggcaaaaggc
     3901 accgatgaaa tcgccctcta tattcgccag gtcgccacgg agaatcaggt ggaagtggtc
     3961 gagttcccgc ggctggcacg ctcggtttac tacaccaccc aggtgaacca gcaaattccg
     4021 tttcagcttt accgggccat cgcccacgtt ctgacctacg tcctgcaaat gaaacactgg
     4081 cgcgagggcg cacagccgcg cccggccctg aacagacata tttccattcc caaagaggtg
     4141 cttaaactgg atggcgaaaa caactaaatc gttccttgcg ctgctgcgcg gcggcaatct
     4201 cggcgtgccg ctggtcatac tgtgtattct ggcgatggtt attctgccgc tgccgcctgc
     4261 gctgctggat attctgttca ccttcaacat tgtgctggcg gtgatggtac tgctggtggc
     4321 ggtgtcggcg aaacgaccgc tggaattcag cctgttcccg accattttgc tgatcaccac
     4381 cttaatgcgc ctgacgctta acgtcgcctc tacgcgcgtg gtgctgttgc acgggcatct
     4441 cggcgcgggc gcggcgggta aggtgataga gtcgtttggt caggtggtga tcggcggcaa
     4501 ctttgtcgtc ggtttcgtgg tgtttatcat cctgatgatc atcaacttta tcgtcgtcac
     4561 caaaggggcc gagcgtattt ccgaggtttc tgcccgcttt accctggacg cgatgcccgg
     4621 caaacagatg gcaattgacg ccgatcttaa tgccgggttg atcaaccagg cacaggcgca
     4681 gacccggcgc aaagatgttg ccagcgaggc cgatttttac ggcgcgatgg acggggcatc
     4741 gaagtttgtg cgcggggatg ccatcgccgg gatgatgatt ctggcgatca acctgatcgg
     4801 cggcgtctgt attgggatct tcaaatacaa cctgagcgcc gatgctgcct tccagcaata
     4861 tgtgttgatg accatcggcg acggcctggt ggcgcagatc ccttccctgc tgctctccac
     4921 cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
     4981 taatcagttg ctggcaagcc cgtcggttct ctacaccgcc actggaatta tgttcgtgct
     5041 ggcggtggtg ccgggaatgc cgcatttccc gttcctgatg ttcagcgccc tgcttggttt
     5101 taccggctgg cggatgagca agcgcccgca agcggcggag gcggaagaga aaagcctcga
     5161 aacgctaacc cgtacgatga cagaaaccag cgagcaacag gtcagttggg aaactattcc
     5221 gctgatcgaa cctatcagtt taagcctcgg ctacaaactg gtggcgctgg tggataaagc
     5281 gcagggtaat ccgctcaccc agcggattcg cggtgtacgg caggtgatat ccgacggtaa
     5341 cggagttctg ctgccggaga tccgtattcg ggaaaacttc cgcctcaagc ccagccagta
     5401 cgccattttc atcaacggca ttaaggctga tgaagcggat attccggcgg ataaactgat
     5461 ggccctgccc tccagcgaaa cctacggcga gattgacggc gtactgggga atgacccggc
     5521 gtacgggatg ccggtcacct ggattcaacc cgcgcagaaa gcgaaggcgc tgaatatggg
     5581 gtatcaggtg atcgacagcg ccagcgtgat tgccacccat gtgaataaga ttgtgcgcag
     5641 ctatattcct gacctcttta actatgacga tattacgcag ctgcataacc gtttgtcgtc
     5701 gatggcaccg cgactggcgg aagatttgag cgcggcactc aattacagcc agttgctgaa
     5761 agtgtaccgg gcgctactga ctgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
     5821 cgtgctggtc gccagtagtg cggtgaccaa agatcatatt ctgctggcgg cagatgtgcg
     5881 cctggcgctg cggcgcagca ttacccatcc gttcgttcgc aagcaggagc tgacggtgta
     5941 tacgctgaat aatgagctgg aaaatctgct gaccaacgtg gtgaatcagg cgcaacaggg
     6001 cgggaaagtg atgcttgata gcgtgccagt ggacccgaat atgctcaacc agttccagag
     6061 cacgatgcct caggtgaagg agcagatgaa agcggcgggg aaagatccgg tgctgctggt
     6121 gccgccgcaa ctgcgcccct tgctggcgcg ctacgcaagg ttgtttgcgc cggggctgca
     6181 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagataatgg gggcgttgat
     6241 gtaggcatcc ggcatcggtg catattgccg gatgcggcgt gaacgcctta tccggcctac
     6301 gggtgagcac ggttttgtcg gcatgataag acgcgtagcg gcgcatctgg caccggtgca
     6361 caactgccgg atgcggcgtg aacgccttat ccggcctacg ggtgagcgcg gttttgtcgg
     6421 catgataaga cgcatctgcg tcgcatcagg catcggcgca cagctataaa atgatacgct
     6481 ccccagcact aaaatccgtt acgtcctccg cccaatcgat gggatataac cctcgcgcga
     6541 catcgcgatg aaacgttgag aatggccaat cactcacttg ctttacccaa ccatgtttta
     6601 ctggatttat ataaatataa tcaacatgat ggcgatagtc ttgggtattg cgaatggcat
     6661 gctcccaaaa acgcggttgc cagatatttt tcaatccaca agcatgggta aattgctttt
     6721 taatttcccg ccagcgcgag gaaaaatcat catcgccttc aggtaatgtc catatgcaat
     6781 gcatatgctc cggcaaaact acccaagcgt taatttcaaa aggtctgtct cgcttaacgt
     6841 taataatagc gttacggagc gtctgaaact gggtggtcaa aagttggttt cgacgattct
     6901 gtaaatttac cgtgaaaaac caggttcccc ccttgatgta ataacgacga tattcagaca
     6961 taacgactcc tttttctcaa cggtaagaag aaaaggaaaa gtcggcaatg atgttgtgtt
     7021 aaatcgggaa agcggctcgc aaaaaatgca cagatttata ggcatgataa gacgcgtagc
     7081 gtcgcatcag gcaccggtac acaaccgccg gatgcggcgt gaacgcctta tccggcctac
//
LOCUS       DAOFSV010000188         7121 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.188, whole genome shotgun sequence.
ACCESSION   DAOFSV010000188 DAOFSV010000000
VERSION     DAOFSV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.188"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..214
                     /locus_tag="QBH27_004273"
     CDS             <1..214
                     /locus_tag="QBH27_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016514.1"
                     /translation="FMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            267..425
                     /locus_tag="QBH27_004274"
     CDS             267..425
                     /locus_tag="QBH27_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDQ7016515.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            466..1350
                     /gene="xapR"
                     /locus_tag="QBH27_004275"
     CDS             466..1350
                     /gene="xapR"
                     /locus_tag="QBH27_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDQ7016516.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(1461..1536)
                     /locus_tag="QBH27_004276"
     tRNA            complement(1461..1536)
                     /locus_tag="QBH27_004276"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1501..1503),aa:Lys,seq:ttt)
     gene            complement(1541..1616)
                     /locus_tag="QBH27_004277"
     tRNA            complement(1541..1616)
                     /locus_tag="QBH27_004277"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1581..1583),aa:Val,seq:tac)
     gene            complement(1663..1738)
                     /locus_tag="QBH27_004278"
     tRNA            complement(1663..1738)
                     /locus_tag="QBH27_004278"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1703..1705),aa:Val,seq:tac)
     gene            complement(1783..1858)
                     /locus_tag="QBH27_004279"
     tRNA            complement(1783..1858)
                     /locus_tag="QBH27_004279"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Val,seq:tac)
     gene            2117..3532
                     /gene="gltX"
                     /locus_tag="QBH27_004280"
     CDS             2117..3532
                     /gene="gltX"
                     /locus_tag="QBH27_004280"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDQ7016517.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(3583..3975)
                     /locus_tag="QBH27_004281"
     CDS             complement(3583..3975)
                     /locus_tag="QBH27_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDQ7016518.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3977..4336)
                     /locus_tag="QBH27_004282"
     CDS             complement(3977..4336)
                     /locus_tag="QBH27_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDQ7016519.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4557..4632
                     /locus_tag="QBH27_004283"
     tRNA            4557..4632
                     /locus_tag="QBH27_004283"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4590..4592,aa:Ala,seq:ggc)
     gene            4672..4747
                     /locus_tag="QBH27_004284"
     tRNA            4672..4747
                     /locus_tag="QBH27_004284"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4705..4707,aa:Ala,seq:ggc)
     gene            4954..>7121
                     /locus_tag="QBH27_004285"
     CDS             4954..>7121
                     /locus_tag="QBH27_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDQ7016520.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGR"
BASE COUNT         1683 a         1650 c         1832 g         1956 t
ORIGIN      
        1 ttttatgacg atggtaaatg gcgtcggcgc atgggttggc tcgattctga gcggtatggc
       61 agtggattac ttctcggtgg atggcgtaaa agattggcat accatttggc tgatgtttgc
      121 agggtatgct cttttcctcg cagtgatatt tttctttggg tttaaatata accatgaccc
      181 tgaaaagata acgcatcgtt cagttacaca ttaaaaggat tcgcagctct gttccttggg
      241 gctgctttta tgataaaggt taattagtga aagatatttt attttcttct ggcgttggtt
      301 ttggcattgg tgctttgttt acaatcgtgc gattacctat tcctgttcca aatgtattgc
      361 cgggtatatt atccatcgtg tttatgtatg tcggatatct ggtggtgaaa tactttatgc
      421 catgataatt taatacgatg tatttattat atggagcact taattatgga acgcgtatat
      481 agaacagatc ttaagttgct ccgttatttt cttgccgtag cggaagagtt gcattttggt
      541 cgcgcagcag cgcgtttaaa tatgtctcag cctccgctca gtattcatat taaagagctg
      601 gaaaatcaac tcggcacgca gctttttatt cgccattcgc gcagcgtcgt actgacacac
      661 gcgggcaaaa tcttgatgga agaatcgcgt cgattgctgg tgaatgcaaa taatgtattg
      721 gctcgggttg aacaaatagg tcggggagaa gcagggcgga ttgaactcgg ggttgtggga
      781 acggcaatgt ggggacggat gcgcccggtt atgcggcgat tccttagaga aaatcctaac
      841 gttgaagttc tgtttcgcga aaagatgccc gcgatgcaaa tggccttgct ggaacgccgc
      901 gaacttgatg ccgggatctg gcgaatggcg acagaaccac cgactggttt taccagctta
      961 cggttgcatg aatcggcgtt tctggtggcg atgcctgaag agcatcatct ctcatcattt
     1021 tccaccgtcc cgctggaagc gctacgtgac gagtatttcg ttacaatgcc gcccgtgtac
     1081 actgactggg attttttgca gcgagtttgc cagcaggtgg gattttcacc ggttgttatt
     1141 cgcgaagtta atgaaccgca aacggtactc gccatggtca gtatgggtat tggtatcaca
     1201 ttgatagcgg acagctacgc gcagatgaac tggcccggtg tcatattccg tccgctcaaa
     1261 gaacgcatcc cggcagactt atatattgtt tatgaaacgc agcaggtgac gcccgcgctg
     1321 gttaaactgc tggcggcgtt ggcgcagtag atgagaggca cgggaactgc cggacatagt
     1381 tggtctgata agcgaagcgc atcaggcaat ttcgcaaatt gcagatagca aaaaaacgcc
     1441 tttagggcgc ttttttacat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
     1501 aaaagtcaac tgctctacca actgagctaa cgacccgaag tggtgggtga tgacgggatc
     1561 gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa tcacccactt
     1621 cggtacttca cattgttaca agaaatctag ctggcgagaa agtggtgggt gatgacggga
     1681 tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccac
     1741 ttctcaattt cttgctacac ggcggagact acataaagta gttggtgggt gatgacggga
     1801 tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcaccccc
     1861 gctgtgtgga gtcgcattat agggagagtt caaaatgagt caacgcattt tctaaagaaa
     1921 ttgttcgttc gtcgtaaatt taagcaagat gatcgcaaaa cagaccgtgt tgcgcaattt
     1981 gtcaacgaaa acaataatgc gtaaggttgc aacccgaact acattgagga atcaggcggg
     2041 agtgatagaa tatcgcccac tcaatttttc caggatttgc cggttgtcgg catctttcta
     2101 aacgtaaggc catttcatga aaatcaaaac tcgcttcgcg ccaagcccaa caggctatct
     2161 gcacgttggc ggcgcgcgta ctgctcttta ctcctggctt tttgcacgta accacggcgg
     2221 tgagttcgtg ctgcgtattg aagacaccga tcttgagcgt tccacgccgg aagctatcga
     2281 agccattatg gatggcatga actggctgag cctggagtgg gatgaaggtc cgtactacca
     2341 gaccaaacgt tttgatcgct acaacgcggt gatcgatcag atgctggaag agggcacagc
     2401 ttataaatgc tattgctcta aagagcgcct ggaagcgctg cgcgaagagc aaatggcgaa
     2461 aggtgagaag ccgcgttatg acggtcgctg ccgccacagt catgagcatc acgctgatga
     2521 tgagccgtgt gtcgtacgtt ttgctaaccc gcaggaaggt tctgttgttt ttgacgatca
     2581 gatccgtggt ccgatcgagt tcagcaacca ggagctggat gatctgatta tccgccgtac
     2641 cgatggttcc ccaacctata acttctgtgt ggttgtcgat gactgggata tggaaatcac
     2701 ccacgttatc cgtggcgaag accatatcaa caacacaccg cgccagatta acattcttaa
     2761 ggctctgaaa gcgccggtac cggtttatgc gcacgtttct atgatcaacg gcgatgacgg
     2821 taaaaaactg tccaaacgtc acggggcggt cagcgtaatg cagtatcgtg atgatggtta
     2881 tctgccagaa gcactgctga actatctggt gcgtctgggc tggtcccacg gcgatcagga
     2941 aatcttcact cgtgaagaga tgattaaata cttcactctg aatgccgtca gcaaatctgc
     3001 cagtgcgttc aacaccgaca agctgctgtg gctgaaccat cactacatta acgcgttgcc
     3061 gccggagtat gttgctactc acttacagtg gcacattgag caggaaaata tcgatacccg
     3121 taacggcccg cagctggctg atctggtgaa actgttgggc gaacgctgca agacgctgaa
     3181 agagatggca cagagctgcc gttatttcta cgaagatttt gctgagttcg atgccgacgc
     3241 cgcgaaaaaa catctgcgtc cggtagcgcg ccagccgctg gaagtggttc gtgacaaact
     3301 gactgcgatt actgactgga ccgctgaaaa cgttcatcac gctattcagg cgacggcgga
     3361 tgagctggaa gtgggtatgg gtaaagttgg tatgccgctg cgtgtcgccg taaccggtgc
     3421 ggggcagtct ccggcgctgg atgttaccgt tcacgcaatc ggtaagaccc gcagtatcga
     3481 gcgtatcaac aaagcgctgg cttttattgc tgaacgcgaa aatcagcagt aattagcgca
     3541 taaaagataa acggcaggag agatacccct gccgtttttt atttatgctg attcatctat
     3601 gtcgagacga gcaagaaaat tacgaattcc ttcgcgtgac aaaaattgcg caactttttc
     3661 ttgctcactg tgagtcatgt tgtctattgc gctgatgatt tgccgataag caggagtgcg
     3721 aatacccggt gcgtattcgg caaggggttc ttcggcttcc agaagcatcg gtgtgttatg
     3781 gaaggctggc gtgttctgaa taaactcgca aacttgcgta tcgaccagaa taagtctggc
     3841 acgaccgcct ttcacgccgg gaaatttttc cgttttccag cctttttctc tgatccaacg
     3901 gttaacagtt tgtttagcca caccgaggca ttcagccagt tcttcggtgg tcattttatt
     3961 gcgtaatctt ttcatattat ttgctatcgc gaatccctaa gcgttgtaac aatccgataa
     4021 tcccttcccg cagaagcaag gatgtaaact gtttttgttc aactggcgtc atctctttcg
     4081 ccagtgtcac cagcaacact tcaagcggcg catcaccaga aagggctggc gcttctccct
     4141 gaccttctgg acgttcagca ttgcgaatat attcacgaac ctgttcattg acgtgaacca
     4201 gtcgagcctt gccaccctgg acgcctggtt ttggtgacgt tgtccagcct tccttgcgta
     4261 cccatttatt aatggtctgg cggctatagc cggtcagtct ggcaagttca tctggcgtca
     4321 tcctctcctt gaacataaca attccctgaa tgtatgtggg tctattagta gttcatttta
     4381 taacaccact ttacaggaaa ccgtgacatg aataactact gaatgcgagc ggttttccaa
     4441 aattgttcat ttgcctgatt tttcagcgat tgaaactaca cgtggcaatt tgccgttgac
     4501 acattcgggc ggaattcata tgatgccgcc cgtcaactcg acaagcttta cgtgatgggg
     4561 ctatagctca gctgggagag cgcttgcatg gcatgcaaga ggtcagcggt tcgatcccgc
     4621 ttagctccac caaattttgc acccagcaaa tttggtacgt aaacgactcg tggggctata
     4681 gctcagctgg gagagcgctt gcatggcatg caagaggtca gcggttcgat cccgcttagc
     4741 tccaccaaat ttcaaaccct cgctgcaaag cgggggtttt ttgtctctgc cttttgccgc
     4801 ttttgtaata cagtctacat cctggcagtg ccgtgtggtg aatgcatcac tgactgcaaa
     4861 aacagcaaca ggctggcccc tgtttgcttc gcgatgcgaa taaacttatt atttgtgtgc
     4921 ctgaaaaccc cgatcagtga gagtagtgta ctcatgtttg tggagcataa cctgataaaa
     4981 aatatcaaga tattcacact agcgtttacg ctcaccgtgg tacttattca gctatcccgt
     5041 ttaatttcgc cacttgctgt tatccattcc aattatatct ttctggcgtg gatgccactg
     5101 tgcgtaatgc tgtcaatctt gtttatcttt ggctggcgtg gtgtcgttcc cattttatgt
     5161 gggatgtttt gcaccaatct gtggaacctt catctcactt ttttacagac cgccgtcatg
     5221 atcggcagcc aggcgtttgc agtcctgtgt gcctgtgcaa tattacgctg gcaattgggg
     5281 acgcgttggc gttatggatt gaccagccga tacgtctggc aacgtctgtt ctggcttggt
     5341 ttggtggcgc cgattggcat caaatgcagc atgtatcttg tgggaaattt ctttgatttt
     5401 ccgctaaaga tatctacctt tttcggcgat gcggatgcca ttttcacggt cgttgatttg
     5461 ctaagccttt tcaccgcagt gctgatttac aacatgcttt tttactatct cacccgcatg
     5521 attgtaagtc cccactttgc acagatattg tggcgtaggg atatcgctcc gtcgttgagc
     5581 aaagagaaac gcgcatttac cttaagctgg ctggcagctc ttagcgtgct gctacttctg
     5641 atgtgcacac cgtatgaaaa tgactttatt gccggttact tggtacctgt tttctttatc
     5701 atctttaccc tcggtgtcgg taagcttcgc tatccgtttt taaatctcac ctgggctgtt
     5761 tcaacgcttt gcctgctgaa ttacaaccag aactttttgc aaggggtact aaccgaatat
     5821 tcgctggcat ttattctcgc agtactgatt tcctttagtg tttgtctgct ctatatggtg
     5881 cgtatttatc atcgtagtga atggcttaac cgccgctggc atttgcaggc gctgaccgat
     5941 ccgttaacgc tcctacccaa ctttcgtgcg ttggaacaag caccggagca agaggcgggc
     6001 aagagttttt gctgcctgcg cattgataat cttgagttta tgagtcgtca ttacggctta
     6061 atgatgcgcg ttcactgtat ccgctcaatt taccgtacgt tgctgccgtt gatgcaggaa
     6121 aacgaaaagt tgtatcaatt gccgggtagt gaactgctgt tagtgctgag cgggccggaa
     6181 acggaagggc gactccagca tatggttaac atcctgaata gtcggcaaat tcactggaac
     6241 aataccgggc tggatatggg ctatggtgct gcctgggggc attttgatgg aaatcaggaa
     6301 accctgcaac ccttgttggg gcagttaagc tggctggcag agcaatcctg cgcacatcat
     6361 catgtgctgg cgctggatag cagagaggag atggtttccg ggcaaaccac taaacaggtg
     6421 ctattgctga ataccattcg cacggcgtta gatcagggtg atttgctgct ctacgcccag
     6481 ccaattcgca acaaagaggg tgaaggttat gatgagatcc tcgcgcgact gaagtatgac
     6541 ggcggcatta tgaccccgga taagtttctg ccccttattg ctcagtttaa ccttagcgcg
     6601 cgttttgatt tgcaagtgct ggaatccttg ttgaagtggc tggcaacaca cccttgcgac
     6661 aaaaaaggtc cacgattttc agtcaattta atgccgctca cgctgctgca aaagaatatt
     6721 gccgggcgga ttattcgtct gtttaagcgt tattacatct ccccgcaggc ggtcattctt
     6781 gagatcaccg aggagcaggc gttttctaac gcagaaagca gcatgtacaa catcgagcag
     6841 ctgcataagt ttggtttccg gattgcgatt gatgactttg gcaccggata tgccaactac
     6901 gaacggttaa agcgtttgca ggctgatatc atcaaaattg atggcgtctt tgtgaaagat
     6961 atcgtcacga acacgctgga tgcgatgatt gtgagatcaa ttaccgatct ggcgaaagcg
     7021 aagtcattga gtgtggtcgc ggagtttgtc gagacgcaac agcagcaggc gctattgcat
     7081 aagctcgggg tgcaatatct gcaagggtat ttgattggtc g
//
LOCUS       DAOFSV010000189         7064 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.189, whole genome shotgun sequence.
ACCESSION   DAOFSV010000189 DAOFSV010000000
VERSION     DAOFSV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.189"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..857
                     /gene="hisG"
                     /locus_tag="QBH27_004286"
     CDS             <1..857
                     /gene="hisG"
                     /locus_tag="QBH27_004286"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDQ7016521.1"
                     /translation="RLSDDSRELLARCGIKINLHTQRLIAMAENMPIDILRVRDDDIP
                     GLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFGGCRLSLATPVDEAWD
                     GPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPRAGLADAICDLVSTGA
                     TLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQGVIQARESKYIMMHA
                     PTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWETMEKLKALGASSILV
                     LPIEKMME"
     gene            863..2167
                     /gene="hisD"
                     /locus_tag="QBH27_004287"
     CDS             863..2167
                     /gene="hisD"
                     /locus_tag="QBH27_004287"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDQ7016522.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2164..3234
                     /gene="hisC"
                     /locus_tag="QBH27_004288"
     CDS             2164..3234
                     /gene="hisC"
                     /locus_tag="QBH27_004288"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDQ7016523.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3234..4301
                     /gene="hisB"
                     /locus_tag="QBH27_004289"
     CDS             3234..4301
                     /gene="hisB"
                     /locus_tag="QBH27_004289"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDQ7016524.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGAQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4301..4891
                     /gene="hisH"
                     /locus_tag="QBH27_004290"
     CDS             4301..4891
                     /gene="hisH"
                     /locus_tag="QBH27_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDQ7016525.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIEED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGTAGAKLLKNFLEM"
     gene            4891..5628
                     /gene="hisA"
                     /locus_tag="QBH27_004291"
     CDS             4891..5628
                     /gene="hisA"
                     /locus_tag="QBH27_004291"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDQ7016526.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVTGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVFLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5610..6386
                     /gene="hisF"
                     /locus_tag="QBH27_004292"
     CDS             5610..6386
                     /gene="hisF"
                     /locus_tag="QBH27_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDQ7016527.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6380..6991
                     /locus_tag="QBH27_004293"
     CDS             6380..6991
                     /locus_tag="QBH27_004293"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDQ7016528.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNILNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLATVIENLRKRHQ"
BASE COUNT         1642 a         1904 c         2013 g         1505 t
ORIGIN      
        1 gccgtttaag tgatgactca cgcgaattac tggcgcgctg tggcattaaa atcaatcttc
       61 acacccagcg tctgatcgcg atggcagaaa acatgccgat tgatattctg cgcgtgcgtg
      121 acgacgatat tcccggtctg gtaatggacg gcgtggtaga ccttgggatt attggcgaaa
      181 acgtgctgga agaagagctg cttaaccgcc gcgcccaggg tgaagatccg cgctacttta
      241 ccctgcgtcg tctggatttc ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag
      301 cctgggacgg tccgctctcc ttaaacggta aacgtatcgc cacctcttat cctcacctgc
      361 tcaagcgtta tctcgaccag aaaggtatct cttttaaatc ctgcttactg aacggttctg
      421 tcgaagttgc gccgcgtgcc gggctggcgg atgcgatttg cgatctggtt tccaccggtg
      481 ccacgctgga agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc
      541 tgatccagcg cgatggcgaa atggaagaat ccaaacagca actgatcgac aaactgctga
      601 cccgtattca gggtgtgatc caggcgcgcg aatcaaaata catcatgatg cacgcaccga
      661 ccgaacgcct ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca accattctgc
      721 cgctggcggg tgaccaacag cgcgtagcga tgcacatggt gagtagcgaa accctgttct
      781 gggaaacgat ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc ctgccgattg
      841 agaagatgat ggagtgatcg ccatgagctt taacacaatc attgactgga atagctgtac
      901 tgcggagcaa caacgccagc tgttaatgcg cccggcgatc tccgcttctg aaagcattac
      961 ccgcaccgtt aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga
     1021 atacagcgcg aagtttgata aaaccacggt taccgcgctg aaggtgtctg ctgatgagat
     1081 cgccgccgcc agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa
     1141 gaatattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgtag aaacgcagcc
     1201 aggcgtgcgt tgccagcagg tcacgcgccc ggtggcttca gtgggtttgt atattcctgg
     1261 cggctccgcc ccgctcttct caacggtatt aatgctggcg actccggcgc gtattgcggg
     1321 ctgtaaaaaa gtggtgctgt gctcaccgcc gccgattgcc gatgagatcc tttatgcggc
     1381 gcaactgtgc ggtgtgcagg acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct
     1441 ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt
     1501 tgtcaccgaa gcaaaacgcc aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc
     1561 cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc gcaacgccgg atttcgtggc
     1621 ttctgatttg ctctcccagg ctgaacacgg cccggactca caggtgattt tactgacacc
     1681 tgatgctgat atggcgcgtc gcgttgccga ggccgtcgaa cgccagttgg cagaattgcc
     1741 gagagctgaa accgcccgtc aggcactgag cgccagccgc ctgatcgtaa ccaacgattt
     1801 agcgcagtgc gtagagatct ccaatcagta cggtccggag cacctgatca ttcagacccg
     1861 caacgcccgc gatctcgtcg atagcattac cagcgccggt tcggtatttc tcggtgactg
     1921 gtcaccggaa tcggcaggtg attacgcctc cggcaccaac cacgtgctgc cgacttacgg
     1981 ttacaccgcc acctgttcca gcctcgggct ggcagatttc cagaaacgca tgaccgtaca
     2041 ggaactgtcg aaagaaggtt tctccgcgct ggcttcgacc attgaaacac tggccgccgc
     2101 cgagcgcctg accgcccaca aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca
     2161 agcatgagca ccgtgactat tactgattta gcgcgtgaaa acgtccgcaa cctgacgccg
     2221 tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct ggctgaacgc caacgaatat
     2281 cccacagccg tggagtttca gcttactcag caaacgctca accgctaccc ggaatgccag
     2341 ccaaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg
     2401 gtcagccgtg gcgcggacga aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt
     2461 aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag cgctgaaacc
     2521 attggcgtcg aatgccgcac agtgccgacg ctggaaaact ggcaactgga cttacagggc
     2581 atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa caaccccacc
     2641 gggcaactga tcaacccaca ggattttcgc accctgctgg agttaacccg cggtaaggcg
     2701 attgtggttg ccgatgaagc ctatatcgag ttttgcccgc aggcatcgct ggctggctgg
     2761 ctggcggaat atccgcacct ggctattttg cgcacactgt cgaaagcttt tgctctggcg
     2821 gggcttcgtt gcggatttac gctggcaaac gaagaagtca tcaacctgct gatgaaagtg
     2881 atcgccccct acccgctctc gacgccagtt gccgacattg ccgcacaggc gttaagcccg
     2941 cagggaatcg tcgccatgcg cgaacgggtg gcgcaaatta ttgcagaacg cgaatacctg
     3001 attgccgcac tgaaagagat cccctgcgtg gagcaggttt tcgactctga aaccaactac
     3061 attctggcgc gctttaaagc ctccagtgca gtgtttaaat ctttgtggga tcagggcatt
     3121 atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat taccgtcgga
     3181 acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt ttgatgagtc
     3241 agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg agtgattttc
     3301 aggtggaccg ttttgacaaa ctcgcctttg aacctggcgt gatcccggag ctgctgaagc
     3361 tgcaaaaagc gggctataag ctggtgatga tcaccaatca ggatggtctg ggagcacaaa
     3421 gtttcccgca ggcggatttc gatggcccgc acaacctgat gatgcagatc ttcacctcgc
     3481 aaggcgtgca gtttgatgaa gtgctgattt gcccacacct gcccgccgat gagtgcgact
     3541 gccgtaagcc gaaagtaaaa ctggtggagc gttatctggc tgagcaagcg atggatcgcg
     3601 ctaacagtta tgtgattggc gatcgcgcga ccgacattca actggcggaa aacatgggca
     3661 ttaatggttt acgctacgac cgcgaagccc tgaactggcc aatgattggc gagcaactca
     3721 ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa agagacgcaa attgacgtcc
     3781 aggtgtggct ggatcgcgag ggtggcagca agattaacac cggcgttggc ttctttgatc
     3841 acatgctgga tcagatcgcc acccacggcg gtttccgtat ggaaatcaac gttaaaggcg
     3901 acctctatat cgacgatcat cacaccgtcg aagataccgg cctggcgctg ggggaagcat
     3961 taaaaattgc tcttggcgac aaacgcggta tttgccggtt tggttttgtg ctgccgatgg
     4021 acgaatgcct tgcccgctgc gcactggata tctctggtcg cccgcacctg gaatataaag
     4081 ccgagtttac ctaccagcgt gtgggcgatc tcagcaccga gatgatcgag cacttcttcc
     4141 gttcgctctc atacaccatg ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc
     4201 accaccgtgt agagagcctg ttcaaagcgt ttggccgcac cctgcgccag gccatccgcg
     4261 tggaaggcga caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga
     4321 tactggctgc gccaacctga actcggtgaa gtctgccatt gcgcgtcacg gttatgaacc
     4381 caaagtcagc cgtgacccgg acgtcgtgtt gctggccgat aaactgtttt tacccggcgt
     4441 tggcaccgca caagcggcga tggaccaggt gcgcgagcgc gagctgtttg atctcatcaa
     4501 agcctgtacc caaccggtgc tgggcatctg cttagggatg caactgctgg ggcggcgcag
     4561 cgaagagagc aacggcgtcg acttgctggg catcatcgaa gaagacgtgc cgaaaatgac
     4621 cgactttggt ctgccactgc cacatatggg ctggaaccgc gtttacccgc aggcaggcaa
     4681 ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca gctacgcaat
     4741 gccagtcaat ccgtggacca tcgcccagtg taattacggc gaaccgttca ccgcggcggt
     4801 acaaaaagat aacttctacg gcgtgcagtt ccacccggag cgttctggta ccgctggcgc
     4861 taagttgctg aaaaacttcc tggagatgtg atgattattc cggcattaga tttaatcgac
     4921 ggcaccgtgg tgcgtctcca tcagggcgat tatggcaaac agcgcgatta cggtaacgac
     4981 ccgctgccgc gcttacagga ttacgccgca caaggtgccg aagtactgca cctggtggat
     5041 ctgaccgggg caaaagatcc ggctaaacgt caaatcccgc tgattaaaac cctggtcacg
     5101 ggcgttaacg tcccggtgca ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg
     5161 ttactggaag caggcgttgc gcgtgtggtg gttggctcca ccgcggtgaa atcaccagaa
     5221 atggtgaagg gctggtttga acgttttggt gccgatgcct tagtgctggc gctggatgtc
     5281 cgtatcgacg agcaaggcaa caagcaggtg gcagtcagcg gctggcaaga gaactcgggc
     5341 gtttcactgg aacaactggt ggaaacctat ctgcccgtcg gcctgaaaca tgtgctgtgt
     5401 accgatatct cgcgcgacgg cacgctggcg ggatctaacg tctttttata tgaagaagtg
     5461 tgtgccagat atccgcaagt ggcgtttcag tcctccggcg gcattggcga tattaacgat
     5521 gtggcggccc tgcgtggtac tggtgtgcgc ggcgtaatag ttggtcgtgc attactggaa
     5581 ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg
     5641 acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa atcattggcg
     5701 atatcgtgcc actggcaaaa cgctacgccg aagaaggtgc agatgaactg gtgttctacg
     5761 atatcaccgc ttccagcgat ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg
     5821 aagtaatcga cattccgttt tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga
     5881 aaattctttc ctttggcgca gataaaatct ccatcaactc tcctgcgctg gcggacccga
     5941 cgttaattac tcgcctggcc gatcgctttg gcgtgcagtg tattgtggtc ggtattgata
     6001 cctggtacga cgccgaaacc ggtaaatatc atgtgaatca atataccggc gatgaaagcc
     6061 gcacccgcgt tactcagtgg gaaacgctcg actgggtaca ggaagtgcaa aaacgcggtg
     6121 ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt tacgacctcg
     6181 agcagctgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg
     6241 gcaccatgga acactttctc gaagccttcc gcgatgccga cgttgacggc gcgctggcag
     6301 cttccgtatt ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac ctggcaacac
     6361 agggcgtgga gatcaggata tgttaacaga acaacaacgt cgcgaactgg actgggaaaa
     6421 aaccgacggt ctgatgccgg tgattgtgca acacgcggta tccggcgaag tgctaatgct
     6481 gggctatatg aacccggaag ccttagacaa aaccctcgaa agcggcaaag tcaccttctt
     6541 ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacg tcgggcaata ttctcaatgt
     6601 ggtgagtatt gccccggact gcgacaacga cacgttactg gtgctggcaa atcccatcgg
     6661 cccgacctgc cacaaaggca ccagcagctg cttcggcgac accgctcacc agtggctgtt
     6721 cctttatcaa ctggaacaac tgctcgccga acgtaaatct gccgacccgg aaacctccta
     6781 caccgccaaa ctgtatgcca gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg
     6841 cgtggaaacc gcactggcag caacggtaca tgaccgcttt gagctgacca acgaggcatc
     6901 tgacttgatg tatcacctgc tggtgttgtt gcaggatcag gatctggatt tagcgacggt
     6961 aattgagaac ctgcgtaaac ggcatcagtg agttgcgtgc tgaccggatg cgatgttgtt
     7021 gtcgcatccg gagccattgc ccgatgcgca agcttatcgt gcct
//
LOCUS       DAOFSV010000190         6948 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.190, whole genome shotgun sequence.
ACCESSION   DAOFSV010000190 DAOFSV010000000
VERSION     DAOFSV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.190"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            280..648
                     /gene="sufA"
                     /locus_tag="QBH27_004294"
     CDS             280..648
                     /gene="sufA"
                     /locus_tag="QBH27_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDQ7016529.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            657..2144
                     /gene="sufB"
                     /locus_tag="QBH27_004295"
     CDS             657..2144
                     /gene="sufB"
                     /locus_tag="QBH27_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDQ7016530.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTVISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            2154..2900
                     /gene="sufC"
                     /locus_tag="QBH27_004296"
     CDS             2154..2900
                     /gene="sufC"
                     /locus_tag="QBH27_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDQ7016531.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            2875..4146
                     /gene="sufD"
                     /locus_tag="QBH27_004297"
     CDS             2875..4146
                     /gene="sufD"
                     /locus_tag="QBH27_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDQ7016532.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPTKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVMARIGQRLPGGAR"
     gene            4143..5363
                     /gene="sufS"
                     /locus_tag="QBH27_004298"
     CDS             4143..5363
                     /gene="sufS"
                     /locus_tag="QBH27_004298"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDQ7016533.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGVLYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALAQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            5376..5792
                     /gene="sufE"
                     /locus_tag="QBH27_004299"
     CDS             5376..5792
                     /gene="sufE"
                     /locus_tag="QBH27_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDQ7016534.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            5940..6944
                     /gene="ldtE"
                     /locus_tag="QBH27_004300"
     CDS             5940..6944
                     /gene="ldtE"
                     /locus_tag="QBH27_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDQ7016535.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
BASE COUNT         1743 a         1687 c         1913 g         1605 t
ORIGIN      
        1 gaacgctgca cttttttacc tatcccttaa atagtcattt tcaatgcaac tttttctttt
       61 tcatcacagc cttaatgctc cttagataat aatcattatc taacaatgag atacctaatt
      121 cttagcgtgc ctgttaaccc acaaatcagg gtctatgctt attaaaagca ggtaaaaagc
      181 atgttttacg ctaaagcccc tgcgtttgct gggttgaact gataatcatt atcactaaca
      241 tgctgttata cgctgaaagc gatgaagtga ggtaaatcga tggacatgca ttcaggaacc
      301 tttaatccac aggatttcgc ctggcaaggc ttaacgctga cacccgcagc ggcgatacac
      361 atccgcgaac tggtggcaaa gcagccgggt atgatcggcg tgcgcttagg cgtgaagcaa
      421 acgggctgcg cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat
      481 ctgctgtttg aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat gccttttatt
      541 gatggcacgg aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac
      601 cctaaagccc agaatgaatg tggctgtggc gaaagctttg gggtataggc ggtactatgt
      661 ctcgtaatac tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata
      721 aagaaggatt cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg
      781 tggtgcgcgc aatttcggcg aagcgtaatg agccggagtg gatgctggag tttcgtctca
      841 acgcctatca cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca
      901 agctgaatta tcaggattac agctactact cggcaccatc gtgcggtaat tgtgacgaca
      961 actgcgcgtc tgaacccggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag
     1021 aggtggaggc ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa gaggtggcgg
     1081 tggatgccat tttcgactct gtttcggttg ccactactta tcgcgaaaaa ctggcggagc
     1141 agggaattat tttttgttcc tttggcgagg cgatccacga tcacccggaa ctggtgcgta
     1201 aatatctcgg caccgtggtg ccggggaatg acaacttctt tgccgcactt aatgcggcgg
     1261 tagcctctga cggtacgttt atttatgtgc ctaaaggcgt gcgttgcccg atggaacttt
     1321 ccacctattt tcgcattaac gcggaaaaaa ccgggcagtt tgagcgcacc attctggtgg
     1381 ccgacgaaga cagctacgtt agctacattg aaggctgttc cgctccggtg cgtgacagct
     1441 atcagttaca cgcggcggtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt
     1501 ctacggtaca aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca
     1561 ccaagcgtgc tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg
     1621 ggtcagcgat tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg
     1681 agttttactc agtggcgctg accagcggtc atcaacaagc ggataccggc accaagatga
     1741 tccacatcgg taaaaacacc aaatcgaccg ttatctcgaa agggatctct gccggacata
     1801 gtcagaacag ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt
     1861 tcactcagtg cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg
     1921 tcgaatgtcg caataacagc gctcagctgg agcacgaggc aacgacatca cgtattggtg
     1981 aagatcaact gttttactgc ctgcaacgcg ggatcagcga agaagatgcc atctcgatga
     2041 ttgttaacgg tttttgcaaa gacgtgttct cggaactgcc gctggaattt gccgttgaag
     2101 cacaaaaact cctcgccatc agccttgagc atagcgtcgg ataaggaata aacatgttaa
     2161 gtattaaaga tttacacgtc agcgtggaag ataaagctat cctgcgtgga ttaagcctcg
     2221 atgttcgtcc cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct
     2281 tatcggcaac gcttgccggg cgagaagatt atgaagtgac gagcggcacg gttgagttta
     2341 aaggcaaaga tttgcttgcg ctgtcgccgg aagatcgtgc aggtgaaggc atctttatgg
     2401 ccttccagta tccggtggaa attcccggcg tcagtaacca gtttttcctg caaacggcac
     2461 ttaatgcggt gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt
     2521 tgatggaaga gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa
     2581 acgttggttt ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg gcggtgctgg
     2641 aaccggagtt atgcattctt gatgagtcag actccgggct ggatattgac gcattaaaag
     2701 tggttgccga tggcgtgaac tcgctgcgtg acggcaagcg ttcattcatc attgttacgc
     2761 actatcaacg cattctcgac tacatcaagc ctgattacgt ccatgtgctg tatcagggac
     2821 gaattgtgaa atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct
     2881 ggcttaccga acagcagtaa cgcactgcaa cagtggcatc atttgtttga agctgaaggg
     2941 gcgaaacgct ccccgcaagc acagcagcat ttacaacaat tgctgcgtac cggactgccg
     3001 acacgtaaac atgaaaactg gaaatatacg ccgctggacg ggctgaccaa tagccagttt
     3061 gtctgcattg cgggggagat atccccacag cagcgtgatg ccttagcgtt aacgttagac
     3121 gccgtgcggc tggtgtttgt cgatggacgt tacgtgcccg cactgagcga tgcaactgaa
     3181 ggcagcggat atgaagtgag cattaacgac gaccgtcagg ggttacccga cgctattcag
     3241 gcggaagtgt ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgtg
     3301 aagcgcggtc aacggccgac aaagccattg ctgttaatgc atatcaccca gggcgtggca
     3361 ggtgaagagg tgaatactgc ccattaccgg catcatctgg atctggcgga aggtgccgaa
     3421 gcaacggtga tcgaacattt tgtcagcctt aatgatgctc gtcacttcac cggcgcacgg
     3481 ttcactatca acgtcgcagc gaacgcccat ttgcagcata tcaagctggc gtttgaaaac
     3541 ccgctcagtc accactttgc tcataacgat ttgttgctgg ctgatgatgc caccgcattt
     3601 agccacagtt tcctgctggg tggcgcagtg ttacgacaca acaccagtac gcaactcaat
     3661 ggcgaaaaca gcacggtgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt
     3721 gatactcgta cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa
     3781 actatcgtca gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt cgcgcagcac
     3841 gccatcaaaa cggatggtca gatgaccaac aataatctgc tgatgggcaa actggcggaa
     3901 gtggatacta aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg
     3961 acggtggggc gtattgatga cgaacagatg ttttatctgc gctcgcgcgg gatcaatcag
     4021 caagatgccc agcagatgat catctacgcc tttgctgccg aactgacgga agcactgtgt
     4081 gatgaggggc ttaaacagca ggtgatggcc cgaatcggtc aacgactgcc aggaggtgca
     4141 agatgacttt ttccgtcgac aaagtgcggg ccgactttcc ggtgctttct cgtgaggtaa
     4201 acggtttgcc gctggcttat ctcgacagtg ccgccagtgc gcagaaacca agccaggtga
     4261 ttgacgccga ggctgagttt tatcgtcacg gctacgcggc ggtgcatcgc ggtattcata
     4321 ccttaagcgc ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta
     4381 ttaatgcccg ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc
     4441 tggtcgccaa tagctggggc aacagcaatg tgcgagcggg cgataacatc atcatcagtc
     4501 agatggagca ccacgctaac attgtcccct ggcagatgct ttgcgcacgc gttggcgcag
     4561 agctgcgtgt gatcccgctc aaccccgacg gtactctgca actggagacg ctgcctacgc
     4621 tgtttgatga gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg cttgggacag
     4681 aaaatccgct ggcggagatg atcacacttg cgcatcaaca tggcgcaaaa gtgctggtgg
     4741 atggcgctca ggcggtgatg catcatccgg tggatgttca ggcgctggat tgcgatttct
     4801 acgtgttttc cgggcataaa ctgtatggtc ccaccggcat tggcgtgctg tatgtcaaag
     4861 aagccttgtt gcaggagatg ccgccgtggg aagggggcgg ttctatgatt gccaccgtta
     4921 gcctgagtga aggcactacc tggaccaaag cgccgtggcg gtttgaagcc ggtacgccca
     4981 ataccggagg tattattggc cttggcgcgg cgctggagta tgtttcggcg ctggggctta
     5041 ataacatagc cgagtatgaa cagaatctga tgcactacgc gctagcacag ctggaatctg
     5101 taccggatct cactctctat ggcccacaga acaggcttgg cgttattgct tttaatctcg
     5161 gtaaacacca cgcctatgat gttggcagtt ttctcgataa ttacggcatt gctgtgcgta
     5221 ccggacatca ctgcgctatg ccattaatgg cctattacaa cgtccccgcg atgtgtcggg
     5281 cgtcgctggc aatgtacaac acccatgaag aagtggatcg tctggtaact ggattgcaac
     5341 gtattcatcg tctactgggg taaaagggag gctctatggc tgtattgccg gataaagaaa
     5401 aattgctgcg taacttttta cgctgcgcca actgggaaga gaaatatctc tacattattg
     5461 aactgggaca gcgtctgcca gaattacgtg acgaagacaa aagcccgcaa aatagcatcc
     5521 agggctgcca gagtcaggtg tggattgtaa tgcgccagaa tgcgcaggga attattgaat
     5581 tgcagggcga cagcgatgcg gccattgtaa aagggcttat tgcggtcgtc tttattcttt
     5641 acgatcaaat gacgccgcag gatattgtca atttcgatgt gcgcccgtgg tttgaaaaaa
     5701 tggcgctcac ccaacatctc accccatcac gctcacaagg tctggaagcg atgattcgcg
     5761 caattcgcgc caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg
     5821 ctcacattgt cgggtggtca atgtttacac tggcctgcac aggagtgttt tggtttcagg
     5881 tgaactaagc gccatccgac gtttcagcgt gagtctccgg caaatacagg aggtttacaa
     5941 tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc ttttagcgcc atccaggctg
     6001 cctgggcggt tgattatccg ctaccaccaa ccggcagccg actggttggg caaaatcaaa
     6061 cgtatacggt gcaagaaggg gataaaaacc ttcaggccat cgcccgacgt tttgatactg
     6121 cggcaatgtt gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa cccggtacga
     6181 cgataactat tccttcacaa ctgttattac ctgatgcacc gcgccagggg ataattgtga
     6241 accttgcaga actgcgcctt tattattatc cgccgggaga aaatatcgtg caggtttatc
     6301 cgataggtat tggtttgcag gggctggaaa cgccggtgat ggaaacgcgt gtggggcaga
     6361 aaatccctaa cccaacctgg acgcctacgg cgggcattcg tcaacgttcg ctggagcgtg
     6421 gcattaaatt accgccggtc gttcctgccg ggccaaataa cccgctagga cgttacgcac
     6481 tgcgcctcgc gcatggtaat ggcgaatacc tcattcatgg caccagtgcg ccggacagcg
     6541 tcggtttgcg cgtcagttcg ggctgtattc gcatgaatgc accggatatt aaagctttat
     6601 tctccagcgt gcggacggga acgccggtga aagtgatcaa cgaaccggtg aaatattccg
     6661 tggaacctaa cgggatgcgt tatgttgaag tacatcgacc actatcggca gaagaacagc
     6721 agaacgttca gacaatgccg tacacgctgc cagcaggctt tacgcaattt aaagacaata
     6781 aggctgtaga tcagaagtta gtcgataaag cgttgtatcg tcgggcaggg tatccggttg
     6841 cggtgagcag tggagcaact cccgcagcca gcaatgcgcc ttcagtagag tcagcgcaga
     6901 atggggaacc agagcaaggg aatatgttac gcgcgacgca gtagcggt
//
LOCUS       DAOFSV010000191         6870 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.191, whole genome shotgun sequence.
ACCESSION   DAOFSV010000191 DAOFSV010000000
VERSION     DAOFSV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.191"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..665)
                     /gene="waaL"
                     /locus_tag="QBH27_004301"
                     /gene_synonym="rfaL"
     CDS             complement(<1..665)
                     /gene="waaL"
                     /locus_tag="QBH27_004301"
                     /gene_synonym="rfaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HDQ7016536.1"
                     /translation="MLTSFKLHSLKPYSLKSSMILEIITYILCFFSMIIAFVDNTFSI
                     KIYNITAIVCLVALILRGRQENYNIKNLILPLSIFLIGLLDLIWYSAFKIDNSPFRAT
                     YHSYLNTAKIFIFGSFIVFLTLTSQLKSKKESVLYTLYSLSFLIAGYAMYINSIHEND
                     RISFGVGTATGAAYSTMLIGIVSGVAILYTKKNHPFLFLLNSCAVLYVLALTQTRATL
                     LLFP"
     gene            complement(675..1634)
                     /gene="rfaC"
                     /locus_tag="QBH27_004302"
     CDS             complement(675..1634)
                     /gene="rfaC"
                     /locus_tag="QBH27_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDQ7016537.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSATL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLINLNSQAVLEKLSSL"
     gene            complement(1638..2684)
                     /gene="rfaF"
                     /locus_tag="QBH27_004303"
     CDS             complement(1638..2684)
                     /gene="rfaF"
                     /locus_tag="QBH27_004303"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDQ7016538.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2694..3626)
                     /gene="rfaD"
                     /locus_tag="QBH27_004304"
     CDS             complement(2694..3626)
                     /gene="rfaD"
                     /locus_tag="QBH27_004304"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDQ7016539.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3840..5036
                     /gene="kbl"
                     /locus_tag="QBH27_004305"
     CDS             3840..5036
                     /gene="kbl"
                     /locus_tag="QBH27_004305"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDQ7016540.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPELIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            5046..6071
                     /gene="tdh"
                     /locus_tag="QBH27_004306"
     CDS             5046..6071
                     /gene="tdh"
                     /locus_tag="QBH27_004306"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDQ7016541.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6310..>6870
                     /gene="waaH"
                     /locus_tag="QBH27_004307"
     CDS             6310..>6870
                     /gene="waaH"
                     /locus_tag="QBH27_004307"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDQ7016542.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGL"
BASE COUNT         1697 a         1638 c         1732 g         1803 t
ORIGIN      
        1 gggaacagga gtagggttgc tctggtttgt gttagcgcca gaacataaag taccgcgcaa
       61 ctatttaata aaaataaaaa aggatggttt ttcttagtat aaagaatcgc aacgccacta
      121 actatcccta ttagcatagt tgaatatgct gctcctgttg ccgttcctac accaaaagaa
      181 atgcggtcat tttcatgaat gctattaata tacattgcat atccagcaat tagaaatgac
      241 agagaatata aagtgtataa tacactctct ttttttgatt ttagctggct agttagtgtc
      301 aagaaaacaa taaaagaacc aaatataaaa attttggcag tatttaaata actatggtaa
      361 gtagcacgaa atggcgaatt atctatttta aacgcagaat accaaattaa atcaagtaag
      421 cctattaaaa atatagaaag gggaagaata aggtttttta tattataatt ttcttgtctg
      481 ccacgtaaaa ttagtgccac taagcaaact atagcagtga tattatatat ttttatgctg
      541 aaagtattat caacgaatgc aattatcatt gaaaaaaaac ataatatata agttattatc
      601 tctaaaatca ttgatgattt aagagagtaa ggtttcaatg aatgaagttt aaaggatgtt
      661 agcatgtttt acctttataa tgatgataac ttttccaaaa ctgcttgact gttgaggtta
      721 attaaatttt ctcttggagc cctacaaacc atctgattct tcccataccc accaattaat
      781 cccggatcgg taggtccata aaccgtgata ttgggtctat ccagtgccgc cgttaaatgg
      841 cttaaccccg tatccaccga cactacaaat ttagccccgg ccagtacgcg ggcaacgcct
      901 tccagactca tcttcggcaa tacttcaaca taagcaaatc cttccgccag tcgtttcgcc
      961 cgttcttcct catgcggcgc gccccacgga agtttaatcc gtattcctga atcagccagt
     1021 aaaccaatca attctcgcca gtgttcttcc ggccagtgtt tatcatctcg ggttgttgca
     1081 tgaagaaata cggcatattc gccagcatct gtaggcaggt tcgtcaggaa atgctgtgcg
     1141 atagcataat cgccctgggt ttgtggtttg ctatagccca gacttttggc aaacagttcg
     1201 cgggtgcgtt ctacggcgtg ctgctgtttt gcaatatgat gtttacgatt gtaaaacagg
     1261 ctggctaacg gttcgcgagc ggtttgccag tccattccat gctttacgcc atgcgccaga
     1321 cgcgtcacca gcgtcgcgct ttttaccagc ccctgagcgt cgataactgc gtcatagttc
     1381 tctgcttgta gcgcttcacg aaacgctttg cgttcagctt ttatgggggc cgagaaccag
     1441 gctttacgcc agcgacgtat tgccacagga ataactcgct caacggcagc gtgccaggaa
     1501 ggaatctgtg cgaacccttc ttccaccacc cagtcaaact taatccctgg gattgcctgc
     1561 tgggcatcag tgagtgcggg cagcgtatgg agaacatcgc ccatcgacga tgttttaacg
     1621 atcagaaccc gcatccgtca ggcttcctct tgtaacaata gcgcgttgag ttcttccagt
     1681 acgcgctggg gagtaatgtc gatcaagctc tggtgataac cctccgccgc gtcaccttta
     1741 cgcactttgt gatagccggt aatcaggcgg atcacgcgtg ctttatggga tagcggtggt
     1801 gtgaagtccg ggctactcgg accatacagg gcaaccagcg gacgattgag cgccgccgca
     1861 acgtgcatca ggccagaatc gttagtaaca atggctttac aggctgcaat caggataacc
     1921 gcttgatcaa gctgtgtttc ccccgccagg ttccgacacc atgcctgctg ctcggtattc
     1981 aaagcggcaa gaatctcatt gcccgcttca tgatctttcg ccgagccaaa cagaaccacc
     2041 tgataacctt catcaatcag ctgctttgcc agctccgcat agtggtagtg tggccagcgt
     2101 tttgccggac caaactccgc acccgggcaa aaaccaatca tcggacgttc tgatgagagc
     2161 gaaaattgat tacaggtata tgatttttca ccttcgctca cctgcaactg cggccataac
     2221 aatggctgcg gcagatcttg tgctgtacgc ataatgcctt tgtcataggc cagcgcgaca
     2281 tagcgttcca ccattagcgg ccaggcttct ttatcgagca cgcgtacatc gttgagtaaa
     2341 ccgtagcgca tctcgccgcg ccagccggtg cgatgaggaa tacctgcgaa gaaaggcact
     2401 aatgcggatt tgaaggagtt aggtaagacg taggcgcggt cgtagcgctt ttcacgcagg
     2461 ctatgaccca gtttgcggcg ttcgccgatt tccagcgctc cgtgaccgag aggcataggg
     2521 atagcttcgt taacttccgg catccgcgat aataatggac ggcaccatgc cggtgccatc
     2581 acgtcgatta tcgcctgggg atagcgcgcc tggagcgtgc gatagagact ttgcgacatc
     2641 atcatgtcgc caacccaaga cgggccgatc accagtattt tcatgcagag ctcttatgcg
     2701 tcacgattca gccaggccat gtattccgtt acgccttcgg caacggtttt gaacggttta
     2761 tcgtaacccg ccgcgcgcag attcgtcaga tctgcctgag tgaacgcctg gtagcggcct
     2821 ttcagtttat ccgggaacgg aatgtattcg atttggcctt tcttgtgata agcaagcgta
     2881 gcatctgcta ctgcctggaa ggattccgca cgaccggtgc cgaggttgaa gatgccggaa
     2941 acgccatttt ccaggaacca caggtttaca tctgccacgt cgcccacata gacgaagtca
     3001 cgtttaaagt tctcgctacc ttcgaacagc ttcggtgatt caccgttatt aagctgggtg
     3061 ttgagatgga aagcgacgct cgccatactg cctttatggc cttcacgagg tccataaacg
     3121 ttgaaataac ggaagccaac aatctgcgag ttcgcttcag gcaggatttg gcgaacatat
     3181 tcatcaaaca ggaattttga gtaaccgtag acattcaacg gtttttcata ttcgcgggat
     3241 tcaataaagt cggaggtgcg tccaccgtaa gtggctgcgg aagaggcata caggaacggg
     3301 atttcgcgct ccagacagta gtgcagcagc tctttggagt attgatagtt gttatccatc
     3361 atatacttgc cgtcccactc ggtggtggaa gagcacgcgc cttcgtggaa aatcgcttcg
     3421 acatcgccga actcttcgcc agccataatc tggatcagga agtcttcctt atccatatag
     3481 tccgcgatat ccagatccac caggttcaca aacttggtgc cgtctttcag gttgtccacc
     3541 accagaatat cggtgatgcc tttatcattc agggccttaa cgatgttgct gccgataaag
     3601 cccgcgccgc cggtaacgat gatcataact gtaaccttcg aattatggag tcagagacaa
     3661 tctcagacac gaatacttct atcatatcac tacacggcgt tggcttcagc cattcgccaa
     3721 aagtgagatt gatggacgtt atttatcctg aattttgcag aagtgttaac gcgtcatctc
     3781 gtcgcgacct agaagtttgg gtaatatgtg ctggaatttg ccctgtctgg agaatcgcaa
     3841 tgcgtggaga attttatcag cagttaacca acgatctgga aaccgcacgg gcggaagggt
     3901 tgtttaaaga agagcgcatt attacgtctg cgcagcaagc agatatcact gtggctgatg
     3961 gaagccacgt tattaacttt tgcgccaata actatctcgg actggcaaat catccagagc
     4021 tgattgcggc ggcaaaagcg ggaatggact ctcatggttt tgggatggct tcggtgcgct
     4081 ttatctgcgg cacccaggat agccataaag agcttgaaca aaaactggcg gcattcctgg
     4141 ggatggaaga tgcgattctc tactcttcct gctttgatgc taacggcggc ctgtttgaaa
     4201 cgcttctgag cgcggaggac gccattatct ccgacgcact gaaccacgcg tctattattg
     4261 atggtgtgcg tctgtgtaaa gctaaacgct atcgttatgc caacaacgat atgcatgagc
     4321 tggaagctcg tctgaaagaa gcgcgtgaag ccggtgcgcg tcatgtgctg attgccaccg
     4381 atggtgtgtt ctcgatggac ggcgtgattg ccaacctgaa gggcgtttgc gatctggcag
     4441 ataaatacga tgccctggtg atggtagacg actcccacgc ggtcggtttt gtcggtgaaa
     4501 atggtcgtgg ttcccatgaa tactgcgatg tgatggggcg ggtcgatatt atcaccggta
     4561 cgcttggtaa agcgttgggc ggggcttctg gtggttacac cgcagcgcgt aaagaagtgg
     4621 ttgagtggct gcgtcagcgt tctcgtcctt acctgttctc caactcactg gcacctgcca
     4681 ttgtggctgc ttccattaaa gtgctggaga tggtcgaagc gggcagtgag cttcgtgacc
     4741 gtctgtgggc gaacgcgcgt cagttccgtg agcagatgtc ggcggcgggc tttaccctgg
     4801 cgggtgccga tcacgccatt attccggtca tgcttggcga tgctgtagtg gcgcaaaaat
     4861 ttgcccgtga actgcaaaaa gagggcattt acgtcaccgg tttcttctat ccggtcgttc
     4921 ctaaaggtca ggcgcgtatt cgtacccaga tgtctgcggc gcatacccct gagcaaatta
     4981 cgcgtgcagt agaagcattc acgcgtattg gtaaacaact gggcgttatc gcctgagggt
     5041 gtgagatgaa agcgttatcc aaactgaaag cggaagaggg catctggatg accgacgttc
     5101 ctgtaccgga actcgggcat aacgatctgc tgattaaaat ccgtaaaaca gccatctgcg
     5161 ggactgacgt tcacatctat aactgggatg agtggtcgca aaaaaccatc ccggtgccga
     5221 tggtcgtggg ccatgaatat gtcggtgaag tggtaggtat tggtcaggaa gtgaaaggct
     5281 tcaagattgg cgatcgcgtt tctggcgaag gccatatcac ctgcggtcac tgtcgcaact
     5341 gtcgtggcgg tcgtacccat ctgtgccgca acacgatcgg cgtcggtgtt aaccgtccgg
     5401 gctgcttcgc cgagtatctg gtgattccgg cgttcaacgc cttcaaaatc ccagacaata
     5461 tctccgacga cctggcttcc atttttgatc ccttcggtaa cgccgtgcat accgcgctgt
     5521 cgttcgatct ggttggcgag gatgtgctgg tctctggtgc aggtccgata ggtattatgg
     5581 ctgcggcggt ggcgaaacac gttggtgcac gcaatgtggt gatcactgat gttaacgaat
     5641 accgccttga gctggcacgc aaaatgggta tcacccgtgc ggttaacgtc gctaaagaaa
     5701 accttaatga tgtgatggca gaactgggca tgaccgaagg ctttgatgtc ggtctggaaa
     5761 tgtccggtgc gccgccagcg tttcgtacca tgctcgacac catgaaccac ggcggtcgta
     5821 ttgcgatgct gggtattcca ccgtccgata tgtctatcga ctggaccaaa gtaatcttta
     5881 aaggcttgtt cattaaaggt atttacggtc gtgagatgtt tgaaacctgg tacaagatgg
     5941 cggcgctgat tcagtctggc ctcgatctct cgccgatcat tacccatcgt ttctctatcg
     6001 atgatttcca gaagggcttt gacgctatgc gttcgggcca gtccgggaaa gttattctga
     6061 gctgggatta acacgaacaa gggctggtat tccagccctt ttgcctgagg ataatccgtt
     6121 aaatatgtaa aatcctgtca gtgtaataaa gagttcgtaa ttgcactgat ctcttatata
     6181 gctgctctca ttatctctct accctgaagt gactctctca ccggtaaaaa taatatctca
     6241 caggcttaat agtttcttaa tacaaagcct gtaaaacgtc aggataactt cagaggtcgt
     6301 cggtaattta tgatgaacag caccaataaa cttagtgtta ttatcccgtt atataatgcg
     6361 ggcgatgatt tccgcacttg tatggaatct ttaattacgc aaacatggac tgctctggaa
     6421 atcattatta ttaacgatgg ttcaacggat aattctgttg aaatagcaaa gcattacgca
     6481 gaaaactatc cgcacgttcg tttgttgcat caggcgaatg ctggcgcatc ggtggcgcgt
     6541 aatcgtggga ttgaagtggc aacgggaaaa tatgtcgctt ttgtcgatgc tgacgatgag
     6601 gtctatccca ccatgtacga aacgctgatg accatggcgt tagaggatga cctcgacgtg
     6661 gcgcagtgca acgctgattg gtgttttcgt gaaacgggtg aaacctggca atccatcccc
     6721 accgatcgcc ttcgctcaac cggtgtatta accggtccgg actggctgcg gatgggactt
     6781 tcttcccgcc gttggacaca cgtggtctgg atgggggttt atcgccgcga tgttattgtt
     6841 aaaaataaca ttaaatttat tgccggatta
//
LOCUS       DAOFSV010000192         6857 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.192, whole genome shotgun sequence.
ACCESSION   DAOFSV010000192 DAOFSV010000000
VERSION     DAOFSV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.192"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(223..3339)
                     /locus_tag="QBH27_004308"
     CDS             complement(223..3339)
                     /locus_tag="QBH27_004308"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDQ7016543.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDL
                     RNQGYEFISVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKINSSYKAKNWSTPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRIATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVAELEQRFPDPASIESEKEKKDFVKLFGEYLRTENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKLAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQHEQLREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQAVFQKIVSFIEK
                     FKGVGGKI"
     gene            complement(3461..4732)
                     /locus_tag="QBH27_004309"
     CDS             complement(3461..4732)
                     /locus_tag="QBH27_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDQ7016544.1"
                     /translation="MSELSYLEKLLDGVEVEWKTLEDISIKISSGGTPKTGVSEFYDG
                     DIPWLRTQEVNFCDIWDTEVKITESGVKNSSAKWIPKNCVIVAMYGATVGKIGINKIP
                     MTTNQACANIQLNEEVAHYRYVFHFLCSQYTYIKSLGTGSQTNINAQIVKNIKIPIPC
                     PDNPEKSLAIQSEIVRILDKFTALTAELTAELNMRKKQYNYYRDQLLTFKEGEVEWKA
                     LGEIGEFIRGKRFTKADYVEDGGISVIHYGEIYTRYGVYTTHSLSQVRADMAASLRYA
                     KHGDVVITDVGETVEDVGKAVAWLGDDDIAIHDHCYAFRHSLNPKFISYYMQTDSFIS
                     EKAKYVARTKVNTLLINGFSKIMIPVPYPKDHEKSLKEQARIVEILDKFDTLTNSITE
                     GLPREIELRQKQYEYYRDLLFSFPKPETVSN"
     gene            complement(4729..6285)
                     /locus_tag="QBH27_004310"
     CDS             complement(4729..6285)
                     /locus_tag="QBH27_004310"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDQ7016545.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSCYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            6468..6689
                     /locus_tag="QBH27_004311"
     CDS             6468..6689
                     /locus_tag="QBH27_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDQ7016546.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            6689..>6857
                     /locus_tag="QBH27_004312"
     CDS             6689..>6857
                     /locus_tag="QBH27_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDQ7016547.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSA"
BASE COUNT         1783 a         1534 c         1487 g         2053 t
ORIGIN      
        1 agaagccaca ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat
       61 caccggcatt caaaatcatt atggccgcga tctatttctg gctgttctct atcacttttg
      121 gcggcatcgt cgctcatggg taaggggagt atattagcca tttggaaccc cacggtctct
      181 tgcgggttta attaagaacc cgcaagaaaa acacgaattg ggctatattt ttccgcctac
      241 gcctttaaac ttctcaataa acgagacgat tttctggaaa actgcctgtt ttttcgtttt
      301 atattgcggg tttaacggac taagttttgg taatgtctcg tttaattctg tgccattttc
      361 ggtggcgtat tcgcgtttta aagacgtgcg aatatagcgt ttcgccgcct cttcattgag
      421 attttcttct ttaatcaacg cttctgcttc acgtagctgt tcgtgttgag caaaggtaaa
      481 gaacgcgtca atgatgctgg ctttgtctgg taaatcatcc aggttcgttt gctgaataaa
      541 atcgaccacc aggccctctt tcgcccggtt ccccaggctt gaacgaatta agcgtttgac
      601 ctcttcgatc atttcgccct tgcctttatt ttgtctgtta tgttcgaaaa tcagtccaag
      661 gatataatcc aggtttattt cctgagactt cagcaaatcg acctcaaaaa ccacgtcatc
      721 ccagtcagtg gttgatttct cttttttctc agcttctttc tcacggcgct gccagtcgcg
      781 aatatcgtta taggcagaac gataatcctg aatcttgcga tcagcaggga gacgaattgt
      841 ttgcaattca gcgagctttt catcatccac ataatgttct gctttgaatt tttctactgc
      901 aacaggatcg ctaagatcga tttgttgcag ggctttcagc gtggcaaatt catcatagtt
      961 ttgcaggatg ttctcggtac gcaggtattc accaaacagt tttacgaagt ctttcttctc
     1021 tttttcactt tcaatactgg cagggtcagg gaaccgttgt tccagttctg caactactgc
     1081 cataaagccg cgcttagctt caccagtagc agcatcagta aagccttcca tatactctgc
     1141 ataactcttt tctaacacta catttttggt gtttttgtca ccaaacagcg ttatggcatc
     1201 aatagttgag cgttccagat cccggaaagt gacgatatta ccgaaggttt tagtggcgtc
     1261 ataaatgcgg ttggtgcggg aaaatgcctg catcaggccg tgaaaacgta agtttttatc
     1321 gacgaatagc gtgttcaatg ttggagcatc gaagccggtt aagaacatcc ccacgacaat
     1381 tagcagatcg atatcctgat ttttaacccg ctgggctaaa tcacgatagt agttctgaaa
     1441 accgttactg tcggtgctaa agttagtttt aaaatggctg ttatattcac gaattgcagc
     1501 gtccagaaac tctttagcac tgctgtccat tgcgctggta tcaaaagttt catcggaaat
     1561 ctcaccaatg gcattttgtt cttcattggc ggcaaaggag aagattgtcg caatacgcag
     1621 cggtttatag gtagccgatt tattagcggc ttcttcttgt aaccgtttaa acgtcgcata
     1681 ataggctttc gcggcatcca cgctgctcac tgccaacata gcattaaaac cttttgagcc
     1741 agggaaggta cggtgagttt tctggcggaa attattcaga atatattgcg taatttcctg
     1801 tatacgcatg ggatgaagaa acgcctgctg attttcagcc gcactcagtt ttttctcgtc
     1861 ggtttctgtc tctaaagact taaactgtgg ccgcacatcg ttgtagtcca ccttgaattt
     1921 aagcactttt tcatctcgaa tcgcatcggt aataacatat gaatgcaatt cacgaccaaa
     1981 tacgctggcg gttgtttctg agcctaaggc gttttccggg aaaatagggg taccggtaaa
     2041 accaaactga taatagcgtt tgaatttctt cttcaggttt ttctgcgctt ctccaaactg
     2101 gctgcggtgg cattcatcaa atataaacac cacttgctga ttgtatacag gcaggtcgct
     2161 ttctgctttc atcaggttat tgagtttctg aatagtggtg acgataattt tgttatcatc
     2221 cttatccaga tttcgtttaa gacctgcggt attttccgat ccattgacac tgtctggcga
     2281 aaaacgctga tattccttca tggtctggta atcgaggtct ttcctgtcga ccacaaagaa
     2341 gactttatca ataaagtcca gttctgttgc cagacgcgcg gctttaaagc tggtcagggt
     2401 tttaccagaa ccggtagtgt gccagataaa gccaccactt tcgggggtag accagttttt
     2461 cgctttatag gagctgttga ttttccataa gatacgctca gtggcggcaa tctggtacgg
     2521 tcgcatcacc agtagcgtct ggctactgtc aaaaacgctg tagttcacca gaacattcag
     2581 cagagtatgt ttctggaaaa aggtagcggt aaagtctttg aggtctttaa tcagcgtgtt
     2641 gtctgatttt gcccaattca tggtgaagtc aaaactgttt ttatcgcgct ttgtcgtgtt
     2701 ggcaaaataa cgggtatcgg tgccgttaga aatgacaaac agttgcagat acttaaacag
     2761 ggaattttcg ctgttaaaac tctctttact gtaacgatgt atctggttga aagcctcacg
     2821 aatcgccacc ccgcgttttt ttagttcgat ttgcaccagc ggtaaaccat taaccaggat
     2881 cgtgacgtca taacggttag catgagaacc cgtctgttca aactgctgga taatctgcac
     2941 cttattgcgc atgagattct ttttatctat caaatagatg ttctcaagac gctcgtcatc
     3001 aaaaataaag tcgcaaatat agtcgatatg gattttacgg gtcttatcca gaatgccatc
     3061 gctcgggtta tccagatact gctccgtgaa acgccgccac tcgctgtcat taaacaccac
     3121 accattgagg ttctgaagct gttcccgaac attggccagc atcgccgact gtgattttac
     3181 ggatataaat tcatagccct gattccgcag gtcctgaatc agttctcgtt ccaggtccga
     3241 ttcgctctgg tagctgtcgc ctgttggctc agctttgatg tacttatcaa ggacgataaa
     3301 gttattggat tcagcaatgg tgtgtgtctg atgagtcata gcgcatcctt tgtgccgtct
     3361 ggcaagggcc ggaagggagt taagggtgac ttccggcacg taaaaaatag tctatataca
     3421 gaccggatgt taaggtggcc cggtcggtag caacggtcaa ttaattactg acagtttcag
     3481 gttttgggaa actgaacagt aaatcacggt agtattcgta ttgtttctgg cgcaactcga
     3541 tttcacgcgg aagaccttcg gtgatggagt tagtcagtgt gtcgaatttg tcgagtattt
     3601 cgacaatgcg agcttgttcc ttaagtgatt tttcgtgatc tttaggatat ggaaccggaa
     3661 tcataatttt tgaaaaacca ttaatgagta gtgtattaac ttttgttctg gctacatact
     3721 ttgctttttc agaaataaac gaatcggttt gcatgtaata ggaaataaat tttggattca
     3781 aagaatgtcg aaaagcataa cagtgatcat gaatagcgat atcgtcatcc ccaagccatg
     3841 ccactgcttt accaacgtct tctacagtct cccccacgtc agttatcacg acatctccat
     3901 gtttggcata gcgtaatgac gctgccatat cagctctaac ctgtgataac gaatgagttg
     3961 tgtaaacacc atatcgtgta tatatctcac cataatggat tacactgata ccaccatctt
     4021 ctacataatc tgctttagta aaacgttttc cacgaataaa ctcaccaatt tcccccaaag
     4081 ctttccactc aacctcacct tctttaaaag tcagcaattg gtcgcgatag tagttgtact
     4141 gttttttacg catgttaagc tcagcggtaa gctcagcggt aagtgcagta aacttatcca
     4201 gaatccgaac gatttcagac tggatggcaa gggacttttc cggattatcc gggcagggga
     4261 tggggatctt aatatttttg acaatctgcg catttatgtt tgtctgggac cctgttccaa
     4321 gggatttgat atatgtgtat tggctacaca agaagtgaaa tacatatcta taatgagcaa
     4381 cttcttcatt aagttgaata tttgcgcacg cttgatttgt tgtcattgga attttgttta
     4441 tgccgatttt ccccacagtt gccccataca tagcaacaat gacacaattc tttggtatcc
     4501 attttgcact agagttttta actccagact cagttatttt tacctcggta tcccatatat
     4561 cacaaaagtt tacttcttga gttctcaacc aaggaatgtc gccatcataa aattctgata
     4621 cgccagtttt aggggttcct ccagatgata tctttataga aatatcctca agggttttcc
     4681 actcaacctc aaccccatcc agcaattttt ccagataact caactcgctc atttctgcac
     4741 ctcgcagcct tcaatttcag ccacaatcgc atcaatatct ttacgcaact ggtcgatttt
     4801 gctgaccgta gttttaagct ctgcatttag ctcagcaata ttgataattt cgcggttatc
     4861 tttcgcttct acatagcagc tcaccgacag gttatagtca ttagcgacaa cggtctcaaa
     4921 cgcgacagat ttcgccagat gagcaacatc ttccttgctg gcaaatacct gcataatctg
     4981 ttcgatatgg gcatcggtca ggatattgtt gttggtctct tttttgaata gttcgctggc
     5041 atcaataaac tgaactttgg tatccgtttt atgtttagac aacaccagaa tattgacggc
     5101 aatggtggtg ccaaagaaca ggttcggtgc gagtgaaatc acggtttcga catagttatt
     5161 gtcaaccaga tactgacgga ttttctgctc cgcgccgcca cggtaaaaaa tgcccgggaa
     5221 gcagacaatc gcagcacgac ctttggcaga aagatagttc agcgcatgta atacaaacgc
     5281 aaagtcagct ttggatttgg gggccagaac gccagccggg gcaaaacgtt catcgttaat
     5341 cagcgtcggg tcatcgctgc caatccattt caccgaatac ggcgggttag aaacgatggc
     5401 atcaaacggt ttttcatctc tgaagtgcgg ttcagtcagc gtattgccca gcttgatatc
     5461 aaacttgtcg tagttgatgt tgtgcaaaaa catgttcata cgcgccaggt tataggtcgt
     5521 atggttgatt tcctgaccaa aaaaaccttc ttcgatgata tggttatcaa actgtttttt
     5581 cgcctgcaac aacagtgagc cggaacccgc tgccgggtcg tagattttgt taacgctggt
     5641 ctgcccgtgc atagccagtt gtgcaatcag cttggagacg tgctgcggtg taaagaactc
     5701 accgcctgac ttaccggcat ttgccgcata gttagaaatc aggaactcat aggcatcacc
     5761 gaacaggtca atctgatgtt cgttgaagtc accaagtttt aacccttcaa cccctttcag
     5821 aaccgcagcc aggcgggcat ttttatcttt aacggtgtta cccaggcggt tactggtggt
     5881 atcgaaatca gcaaacaaac ctttgatgtc agcttctgaa ggataaccgt aagcagaact
     5941 ttcgatagca acgaagatgc tgtttaaatc tgcattcaat ctgtcattgg tatttgcttt
     6001 cgcagctacg ttgcagaaaa gctggcttgg gtagatgaag tagcctttgg ttttgatggc
     6061 atcgtcttta atgtcatcag taattacgct gtcatccagt ttcgcataac agatactgtc
     6121 atcaccggct tcaatataac tggaaaaatt ttcgctgata aaacggtaga aaagtgcgcc
     6181 cagaacgtat tgcttaaaat cccatccatc gaccgaaccc ctgacatcgt tagcaatttg
     6241 ccagatttga cgatgaagct ctgcacgttg ttgaatactt gtcattttca tccacttatt
     6301 tcaggcttat gtaattggcg gtgattctac agcaacttgg atgctttagc agttcggaca
     6361 ttaggctacg aatgacctgc ctagaggttt gttaagccgc aaagtgctgg tgctttatgc
     6421 ctgtgaagtt tataattgtg tacacataac gagtacacga ggtgtttatg caatccatta
     6481 acttccgtac cgcgcgcggc aacctttctg aagtgctcaa caatgttgag gccggggaag
     6541 aggttgaaat cacccgcaga ggccgtgagc cagcagtaat tgtcagcaag gctactttcg
     6601 aagcctacaa aaaagcggcg ctggatgctg aatttgcatc cctgtttgac accctggact
     6661 ccaccaacaa ggaactggtt aaccgataat gaggcatata tcaccggaag aacttattgc
     6721 gcttcatgat gcgaatataa accgctacgg cggcctgccg ggaatgtctg atccgggcag
     6781 ggcagaggcc attatcggga gagttcaggc cagagttgcc tacgaagaga tcaccgacct
     6841 tttcgaagtc tccgcca
//
LOCUS       DAOFSV010000193         6843 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.193, whole genome shotgun sequence.
ACCESSION   DAOFSV010000193 DAOFSV010000000
VERSION     DAOFSV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.193"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            393..1139
                     /locus_tag="QBH27_004313"
     CDS             393..1139
                     /locus_tag="QBH27_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDQ7016548.1"
                     /translation="MKKVIVVSALAMAGVFSAQALADRGKTGFYVTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSHY
                     TLDTWHSPMGDGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDISALHYSGYDNNFAWSIGAGVRYDITPDIALDLSYRYLDAGKSS
                     LSYKDAEGDKYKSEADVKSHDIMFGVTYNF"
     gene            1314..2813
                     /locus_tag="QBH27_004314"
     CDS             1314..2813
                     /locus_tag="QBH27_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDQ7016549.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTTDEAAEF
                     TGMIPVYHFLVSAAIQVFMVIFWRTHRRGHRNWLALLLFVLCSINSWPLRMVKGTVVW
                     TTDTLREMQRYKQLNQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWPNHYLEQYPRKVACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPDRR"
     gene            complement(2922..6437)
                     /locus_tag="QBH27_004315"
     CDS             complement(2922..6437)
                     /locus_tag="QBH27_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ7016550.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVVLRPKVYFRLLQHGKDRSAGAPDIVTPLVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSINFDDSIDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGTGAMAGRWLPELKSFGAYFPSSTRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKECSSPEKVIELLHGQLVAKSEQLKRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMARIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCFDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGEQASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTSETKIYTLHGVEQHDNFLNQTF
                     ENTDKHITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDFEKRKE
                     KQQNLKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1735 a         1651 c         1505 g         1952 t
ORIGIN      
        1 atctttggag caatggaaca aattgtcgcc cattgtgagt catattcgct ctgactttcc
       61 agaaccatac ggactgcccg ttgacggact tcgggggaaa aacgagtatt tttagtcatc
      121 ctgtttacct ctttctcagg aagtttagtc tccaggattc ccggggcggt tcaatctcgt
      181 gctgttctgg tctcaatact tccatgccgt tgcaattacc tcttaaacca ttccttacca
      241 ttttcatgtc ttcgataaga aatcttcatc atgcatgatc gattttgtcg atcgattcga
      301 tcgaattttc tctttttacc ctgtcttttg catggtttta aaataagcaa ccttctttat
      361 cgtgcgggta agaaccaacg gagatgattg aaatgaaaaa ggttattgtt gtttcagcgc
      421 ttgctatggc gggcgtattt tcagcccagg ctctggctga taggggtaaa acaggctttt
      481 atgtgaccgg taaagctggt gcttcggttg tgactcagac tgaccagcgc ttccgtcagg
      541 actttgggga tgatgtttat aagtataagg gcggtgataa aaacgatact gtatttggtg
      601 caggccttgc ggtgggctat gatttttatc aacattacaa tgttcctgtg cgcacggaag
      661 tggaattcta tggccgcgga gctgcagact cccattatac actggataca tggcattctc
      721 cgatggggga tggtggtcgg gaagacacac agaacaggct cagtgtgaat accctgatgg
      781 tgaatacgta ttatgacttc agaaacagca gtgcattcac tccatgggtg tctgttggac
      841 tgggttatgc acggatacat cataaggcaa catatactga tacctcctgg aataaatctg
      901 gtgaggtgag cgatatttct gcgttgcatt actcgggata tgataacaat ttcgcatgga
      961 gcattggggc cggtgttcgc tatgacataa ccccggatat cgctcttgac ctcagctatc
     1021 gctatctgga tgctggtaaa tcctccctgt cttacaagga tgcagaaggg gataaatata
     1081 aatcagaggc tgacgttaaa agtcatgaca tcatgtttgg tgtgacttat aacttctgac
     1141 gacactgctc ctgagcgata attacacata ttcagtaatt aagatattta tatatcttct
     1201 gcaactaaac agaaataccc tgcagtaata taccgcaggg ttatttgttt cacaggagat
     1261 ttatggtttt tattatctta ttccctttat aataatatta ccgatatatt ccgatgaata
     1321 tcagaaaact gttttgtccg ggcaatacac cccggatttt attgttttta ttcttttttg
     1381 ttgtttctgt cataaccaca attgcatgcg gatacactga gaagaatgcc accgggaatg
     1441 tgttgctgct gttccttctt ctgctccttg cacacagaaa taccctcaca tccattacag
     1501 cgctgttatt tctgttctgt tgtgcactgt atgcgcctgc cggtatgacg tacggtaaaa
     1561 tcaacaacag ttttattgtc gcgttgttgc agaccacaac tgatgaggca gcggagttta
     1621 ccgggatgat tcctgtttat cattttctgg tcagtgccgc gattcaggta ttcatggtga
     1681 ttttctggcg aacacaccgc cgtggtcacc gtaactggct ggcactgctg ctgttcgtat
     1741 tatgctcaat aaacagctgg ccgttgcgga tggttaaagg aactgttgtg tggacaactg
     1801 acacattgcg tgaaatgcag cgttataaac aactgaatca gcacggggct gacaactgga
     1861 aaatcctgcc gggtgtaccg ttgtatgaca cgattgttat cgtcactggt gagagtgtgc
     1921 gcagggatta tatgtcggta tatggctatc ccgtgccaac cacgccgtgg ctgaatacag
     1981 ctcccggttt atttattgac ggctatacat cggcagcagc cagtaccgta ccttccctga
     2041 gccggacact gatttatgac tatgagcaga accctgactc cggcaacaac gtggtggcgc
     2101 tggcagcaaa agcaggatac agcacatggt ggatatccaa tcagggaaaa ctgggggagc
     2161 atgatacacg catctctgtt attgcttctg atgcggagca taccgttttc ctcaagaaag
     2221 gcagctttgc ttcccgtaaa acggatgaca tgttgttgtt acaggaaaca gaacgtgcac
     2281 tggcggataa atcctctccg aaggtgatat tcctgcacat gatggggtct catccgaacc
     2341 cgtgtgaccg gcttcactcc tggccgaatc attacctgga gcagtatccc cgaaaggttg
     2401 cctgttacct cgccagcatc agtaaactgg ataactttct cggtcagctt gatggtatcc
     2461 ttcgccggca ttcccgtcat ttcgccatgc tttacttttc tgaccatgga ctgtcggtca
     2521 gcgacagcgc taatcctgtt catcatgatg gtcatgtgca gggaggctac agcgttcccc
     2581 tgattattac cgccagtgac atcacgtctc atcagtccgt cagcagaaaa atcagtgccc
     2641 gtcatttcgc aggtattttt cagtggctga ccggcattcg tactgaaaat ataccgccat
     2701 tcaatccgct gacagacgaa gataatgaac ccgttatggt ttttaacggg gagaggaatg
     2761 taccggcaga cagtctgaaa ccgcagcctc tgattcttcc ggacagaaga taatcatgtc
     2821 ggtatgaatg ttataattaa ggaccgtgca ataatagcca ttccattcag ccatcttacg
     2881 ctcgaaaaca atcgataaga tggctactca ccattcctgc tctaaacctg acgcctctca
     2941 agactattat aaatagcctc aatctcaccc ttcaggttat caccgcaata aaccattgag
     3001 gtatcgtatc gttcatatcg cgcttcccgt gtcgcactaa accagttgaa cgatcccacg
     3061 catagcaaac catcatcacc aataacaatt ttgctatgaa cacggtttac cagctttgta
     3121 gcaataccca gcgcattcag tttctccagc gccgctttaa ggttctgctg cttctctttt
     3181 cgcttctcaa aatctttatg ttcagtgttg tagcttctgt cagtgactat cgtgacgtta
     3241 ataccacgtg aacacgccgc aatcatagaa tcaagaaaac cggtttgctc cagcttttgc
     3301 caggtcagcc atggagaaac tatcgtgatg tgtttatcgg tattttcgaa cgtctgattc
     3361 aggaagttat catgctgctc cacaccatga agtgtgtaga ttttggtctc ggaagttttt
     3421 aaatccttac gctctttata atcaaaagag agcgcattct tctctgactc aaagaggtat
     3481 tttgccagta atccccgtgg agatgaggct ggctggacct caaacagatc catatcgccg
     3541 aagaccagga agctatcctt cgcacgggag actgcaacat tcagcatgct gttatcgcta
     3601 tcaataaacc cgccgtcttc atgttttgaa tagactggcg agaatataac aatcgctctt
     3661 tccgctccct gaagagagtg cacggtgccc actgtgagcg acttttcatt cgcgcccgta
     3721 ctgatacctt gtttacccag cgcctgtttg atagtggata cctgcgcgct aaaaggcgtc
     3781 acgataccga caacttcatg aagcgatttg ccgtaatgcg cttcaatatt ttgctggttc
     3841 tctgccaacc aggccgctat cgtttcagcc tcaagcaaat tatatctact tccactactt
     3901 gcctgctctc ctttaccatc aatatggaga taacccattg cgggcattaa attgctctct
     3961 tcacgccctc ttttaggcaa caacttaccg tgatagcaga gcgtattaca gtatccaata
     4021 atattatcga agcaccggcg gtgttcatat aggtacatac cacgagccag ttcgggatca
     4081 tattgatagc gcgaagcaaa ctgcgctatt ttcataacgc tgccagatgc ggcactttta
     4141 ccaaggtctg cgattgccgt atatttctcg gtaatctctt cttgcgtact gccagacaga
     4201 attttttccg caagcatgtt accgacatca atcgcgggag caatactcca tattggcggg
     4261 atctgctccg tatcgccaat cactaatgcc ttcttagcta atgcaaacga ggcagcagcc
     4321 acttcaggaa gcacctgccc ggcttcatcg acaatgagta aatcggcaaa atcatacaaa
     4381 taacttttct cgaatttacg ttgtcctttg tgctcactta tctgcatatt gccgggcagc
     4441 atatagcatg tcatcaccac acatggagtg agtttcattc gacgttgcca acgggcggtt
     4501 acccctttag cgcctttttt cttcttctct tcctgcagat catcaattct ggccatatcc
     4561 atcagccaac gaccttccca gtagtgagtc gtcagtaaaa atgcagggaa gcgaatctgc
     4621 gtatcagcca gttcatcggc ctgtgagaag ctcagttcct cgtcgccctc ataccctaaa
     4681 tcaaatgcca gcctctgcca ctcctgaacc gcctgctgtt ctttaagaac gatttcatgg
     4741 gcggagtcaa tctgctgccg gtatgttgtt tgctcgcgct cagcggaatt gagcagccca
     4801 tcaatattac gttcgatagt ttcaggatca gaccactgat ttcctgcaat cagcgcgcct
     4861 aatttatctt cgagaaacag ctgtatttgg tactgtcgct tactacgaac cgctgggagc
     4921 caggaaaata atgaatagat cagtgattca ccggcgcgat attttttcca ttccgtttta
     4981 gcactcttca gtagagtgac tttttgttct tgtccggaaa gtaatttatt taaattatcg
     5041 agatattgct caatatcatt agcaataagc tcacgcgcag cccgtacctg gcttaacgtt
     5101 tgccatgttg cgttcagtct tttcaattgc tcggattttg ctaccaactg accatgcagg
     5161 agttcaatga ccttttcagg ggatgaacac tctttttcag gaaaagctgc cttagctttc
     5221 tcgagataaa acagcagtgc atcctctaca tactcttttg actcaacctg gttgaagaaa
     5281 tcttcagttt gatatttttt ggctgcctca gctttacgag tgcttgaggg aaaataagcg
     5341 ccgaagcttt tcagctcagg taaccatcgt ccggccattg caccagtgcc ctgtgaaaaa
     5401 tctttcccga acgcttcgat aatgttcgtt acagcctggt tgttcgttga agtcgcgata
     5461 ataaccggag gctcagattt ttcgagagcc gctctggccc actgcgtggc gatgatagaa
     5521 agcaccagcg tggtttttcc ggttcccgga ggtccattaa cagcaaggat atcgccatgt
     5581 cttgcatcca gaaaatggct taaggcatcg cgctgagcct ttgccagcgg aaacttatct
     5641 ccggagtgtc caagcctgtc gctgaatttt gctcctgggg ccagcaaaga ctctgcagca
     5701 tgaacctctc gcgaggcgaa gcgattgaag agcggcacat ccttcttgca aacaagcagg
     5761 tgatcataaa gcgaaaggat atggaaactg gcaccaccag attgcgccgt tttaacaata
     5821 taaccgtgct cagcgagttc atattgctca ggatttttaa tccagtcgcc ggcaacgttc
     5881 ttcagcagcc tctctgaatc atccagatat tgacgccact cctgctgcaa ggctgcatat
     5941 cgtgcttccc gttcttcatc cgtttcggca gtcttatcaa tgctgtcatc aaagttgata
     6001 gagaacgagg tatggattgt cttgtatttg tcatactgcc caatctcacc aatcgaaaat
     6061 gctccttttg gcaaaggttc aagcaggtct ctgggaatgg aggtcgctgg cgtcggatat
     6121 aaaaaaccct cacggcttag caaggctggc gtcactagcg gggtaacaat atcaggtgcg
     6181 cctgcggaac ggtccttacc atgctgcagt aaccggaaat aaacttttgg gcgcaagacg
     6241 acatcgaccg tttcgacatc gtctttttct ccctcaaaaa atttactgac aatcgcttcg
     6301 tcaagacgtc ccgccgctat cccatgccag tgagtgaaat ttttggcgtc tttccgttca
     6361 aaactgccct ttcctgactc agcatccgca agcgagttgc gccagtatga ggcaaaccct
     6421 aaagcatttt catccattac cagtcccatg actcaatgaa atccattgat ttatcgaata
     6481 taaaatcacc gtatggacag ctattgtcca tattgaaaaa gttggatgca atgttttgtt
     6541 tataattcga tatcaaatga actggttgta cgaggttcag aacacaagct cattaaacag
     6601 tacgtcagga ctgttgattt cataacagtg aatacatttt ttaccgttta taataattgc
     6661 ctgtgttgga cggggatacc agagcagccg aaaacaccgg gggttattac ctgctctcat
     6721 ggaaaccata tattcctggt gctaatcaca gtcataaata gcctgaagaa catgacgaag
     6781 ggactttaat ggacgccgga gtaacttgcc agaacatcaa tcttcgtcat catttacaaa
     6841 gaa
//
LOCUS       DAOFSV010000194         6837 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.194, whole genome shotgun sequence.
ACCESSION   DAOFSV010000194 DAOFSV010000000
VERSION     DAOFSV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.194"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(261..1133)
                     /locus_tag="QBH27_004316"
     CDS             complement(261..1133)
                     /locus_tag="QBH27_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDQ7016551.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGYSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2465..3955
                     /locus_tag="QBH27_004317"
     CDS             2465..3955
                     /locus_tag="QBH27_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDQ7016552.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(4076..4648)
                     /locus_tag="QBH27_004318"
     CDS             complement(4076..4648)
                     /locus_tag="QBH27_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016553.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(4734..4940)
                     /locus_tag="QBH27_004319"
     CDS             complement(4734..4940)
                     /locus_tag="QBH27_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ7016554.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARITQSRP"
     gene            5888..6037
                     /locus_tag="QBH27_004320"
     CDS             5888..6037
                     /locus_tag="QBH27_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDQ7016555.1"
                     /translation="MEKVISHTRYKLTPAELEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            6766..>6837
                     /locus_tag="QBH27_004321"
     CDS             6766..>6837
                     /locus_tag="QBH27_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDQ7016556.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVH"
BASE COUNT         1775 a         1518 c         1753 g         1791 t
ORIGIN      
        1 ggttacagtg ccggaggtcc ggctgacgga agtttctgac gtgtggctct gttgttccct
       61 gaatctcatg tctgtgtgta tctgcgcacg ttgctaacag gagttattat tagcataacg
      121 atcgatatta acgatcggta aaatgggtat tgatcgtttt attcttttat caagctattg
      181 cacggacaag agagacagta acgaaaacag cgcttttgtg agcatactta gaggcaggga
      241 ggacatcatc cacaacaggt tcagaagaag atccagttcc ataccgcgcg ggcaacagag
      301 accactgtgt cacggacggc acgcagaacc gtgcgtgcaa cagaggcaac acagacgctc
      361 tccgctgtgt caaatatccg gtccaccgca ccggtaaact gctcacgggc ctgagcgcgg
      421 acagattccg tgcgcagctc gtcctgcagg cgggtcatca ggggactggc ggcatggtcg
      481 ggaagcgctg tcatgagcgc actgaccagc gtatccactt cccagccggt gcgggccgat
      541 acggccacaa ccggatgtac aggccggaac agacggaata ccgcctccgt tttttcgcga
      601 atgttctgtg cctgtgcggg agagggctga atgccggcca tatcccattc atggcagggc
      661 tccgttttgt cggcctgtgt caccacaaac agcacctgct gatgtccgca ctgcaggatg
      721 tgtcgccaga aatactcatc cacagacagg gcccggtcat cggctttaat cagccacagc
      781 accaggtcca gttcaggcag aatgtcacgg tacagggctt catactctgc atccctgtcc
      841 cggctctcgc ccaccccggg caggtcagtg ataaccatgc tgtaaccgtg accactcaga
      901 cggaagcgct gcacttcccg ggtgccggcg tgaacatcac tgaccggtgt gacctccccc
      961 tgaaacaggg cattacagag tgaagattta ccggccccgc ttttacccat aatgccaatc
     1021 acgggctcgt gacgggtgag tttgcgcaga tgttccagga tgtgacggga aagcgagtaa
     1081 ggcagggagg agagcggttt ttcaattgcc tcaatggcat cagacggatt catacatgta
     1141 ttcccggtga aaaaagacaa aaccccgcac accggaaagg tggcggggga tccacggaga
     1201 taatatctgt ctgaaattgt ttggaatcga tggggatgtt gcgtattaac atgaaggagg
     1261 gcaggagtta tccgttattg ggtgatatcc ttccatgaaa atacgcggtt attgttaaac
     1321 agcaggctga ttactccgta aaggagaaaa atattcatct tgcagcctga ttatcaggtg
     1381 tgtttatgtt tactgaggtg agtatttttc agtatattgt cggggcagtc agtgggagat
     1441 attaaattta cgtttagagt tatcttgctt ctgctgtgta tttttataag agtgcgcacg
     1501 taatgatatc tgttgatacc atgttgttaa gtgtttccct tccgggtcat gcgtacttgc
     1561 aaaatggcat ctgaatcgat cacggtttgg ccaaaccgtg atcgaataga taggaatacg
     1621 cacgtaatga ttaaatgctt ttgttacacc attcttccgg catgaatatt tatccagttc
     1681 ccttttttat ttgtgtagtg atactgtcgg aatagcgttc agtaatgtga agtgtcctgt
     1741 aaaaaaactt taataaatgt gtcatcctgg tcaggaaata tctgctgaga tattattagt
     1801 tggtggtgtt tttactgagt aatagctggc ttttaattcc tgaactgaaa tactgtcgct
     1861 cgtcagggtt taattaaaac tggatgatga tgtgaaaatg aaggctggtt tttgtgtttg
     1921 tcgaagaagt gttattttaa tccattctca tcattggctg gcgattatac agaagtattt
     1981 gaagaaaaaa tgttgcattt tatctgtgtc agcagggagg aagtatttta ttgatgtatc
     2041 agtattaaat ggtttttgtt atttataaaa atgtcatcct gtccatagaa tattgtactc
     2101 agcattcagt gcaggaactg tcattttctt tattttattc ttcttcagga gggcagtttt
     2161 ctgaaagcgt tactccgggg aaactgttta tgtttattcc cggcaggaaa cccgtacctg
     2221 caataccctg gatgatgctt tttgctggta cgttgttgtg tgaaatttaa tcaacgtgtg
     2281 tgttccggag agcgcccgta gacgcacaga ttcattctga cattctttag cgacaaaata
     2341 ccgaatggtt attatttaat ctgaaaatcg acagaaattt ttatcctgta ttgattctgc
     2401 tgctattttt gctattagta gcaatagttg cggtacttta tgttctgtgt gttgtgaggt
     2461 cattatgcca tactttcagt atcctgatga gtttcccctg agctcgctgc ctccactcat
     2521 tcgtgatgcc gttattgaag ctcagcaaat cacgcaggct cccctggggc ttgtggcagc
     2581 atctgccctg ggcgctgttt cacttgtctg ccagaatctg attgatgtct gcagactgaa
     2641 tacactacgg gggccggttt ctcttttctt actgacgctt gctgagtccg gagaacgaaa
     2701 aaccgcagtg gacaaattat taatggagcc cctgtaccag caggagatgc tgttatattc
     2761 cagacacaaa aatgaactga cgacatggaa aaataaggaa gagcttttaa aggcgcagaa
     2821 aaaagcattg ttgtcaaaac tgaataaaga actccgcaaa ggcgcggatg agtcagaaac
     2881 gctcagacaa ctggaagcgc ttcagaaaaa cagaggggaa aaacctgtaa gatataaatt
     2941 catttttaat gatgccacga ctgcggcaat taagaatcag ctttgtggta aatggcgttc
     3001 agtgggtatt atgtcagatg aagccggtat aatatttgat gggtataccc tgtctgagct
     3061 gccttttatt aacaaaatgt gggatggctc cgtattgtcg gtggacagaa agaatgaacc
     3121 cgaacagatg attgaaaatg ccagaatgac attatccctc atggtacaac ccggtctttt
     3181 tgaccgatat atggagcgaa aaggttctgt tgccagggac agtggttttc ttgcccgttg
     3241 tctaatcagt aaacctgcca ccacgcaggg gaaacgcttt attaacggag ctgtgacccc
     3301 gggaggatca ttaacggcat ttcatgaacg attaatggag ctggccagag gcagtattga
     3361 aaaatccagt aaggatgaac gttattgtct gcatttttct cctgaagcgc aaaaaatatt
     3421 tattgatcac tataatgttc tggagcagga tttgtctcct tccggaccct taagccagtt
     3481 tcggggacat gtttcaaaaa aaacagaaaa cattgcgaga attgctgcgt tattgcagta
     3541 tttcagtcag ggtgaaggga aaatctctgc cgatatcatg acgtctgctg tcgtaataag
     3601 ttcgtggtac acagatgaat ataaaaaatt atttgcgtta cctgatgaat ctgaactgca
     3661 gctgcaggac gcccgggagc tgctcgactg gctcattgag gagtgccgcg gagaatgccc
     3721 gccccgtgtc aggaaaaatt atattctcca gtgtggcccg ggccggttcc ggaacaggaa
     3781 aaagctgaac gtcctgctga acattcttgc atctcaactc aggctctctg tcgtgcagga
     3841 aggaaaaacc acctatgtcc tgctttccga aataaacaat atcacgctgc ctgaactgaa
     3901 tgcgtgctac agccagaaac tgataagatg gcatgagcaa tcctttggct tataatgttc
     3961 cctgttgctg agacggatta ttcagtggtg gtatcgcagt atgcccgtca cccccgcttc
     4021 tgcatgaggg tgacggagga aaagtaagtg tgcggaggcc cgggagcgtt atgaatcaac
     4081 aggcgtataa caggtactcg tcaatcatca gttccagctc gcgcttttcc cgtttgcgcg
     4141 ccacagccca gttttcaagc tctgcatgtg atttatgctt taactcttca gtccagggct
     4201 gcatggagtg ggcacacatc agatcatacc agcacagcca tatcagcgtg tggtagagat
     4261 cacgatcgtc gaaacgccgg agcaggctgt tcagcctgtt tgccagccgg ttttcaccca
     4321 gacgatgtag ctcactgagt aaaacgccgc ccagattaaa atgtgtaagc agcatgattt
     4381 catgggtctg aatgctggtc attaaccctt cctgaatggc aggaatgaac ggtggtgttc
     4441 ccgggttgac gacctgatag agtccctgtg gtgccactga gatgatggtg cctttttctg
     4501 gcagaaaaaa gtgggccagg gtcaggcggt gataaccatg aagatgcgga gcccccattc
     4561 catgagcgac gataagcctc gtcacgtttg tcatccattt ctgatgctgg atgagcagca
     4621 gctcacaccg gttttcaagt ttttgcataa tattctctcc tttagcgcgc agcaaaatgc
     4681 ctccgggggc cggatcatgc ctttgctgcg gttgaacgga taacggtcag gaattacgga
     4741 cgggactgtg taatacgtgc ctgcaaccag gcttccactt cacttttcag ccatcgggag
     4801 ctacggccca gtttgatggg ggcaggaaag gcaccatcct tgatgagctt gtaaaaccat
     4861 ttatcggtca ggccggtcag ctgagtgata aacgacatgt cgaccatctg gtcatccatc
     4921 agtgaaactg gggtattcat gaaaatgttc ctccggatgt tgttaatctc aaccggcgtt
     4981 ggtgatgaaa acaacgcacc accttccccg gcataacaac cggggaaaaa cgtaaaaaat
     5041 aatcgggaat gtgaccgtca ggagataaac ggggggattg cggtggctga gccgtaccgg
     5101 tatgtcgggt gattgttgcg aaacgatgct tccgcacgcg cacgtgataa aggagggagg
     5161 actttcgtga gacaagggga aaactggcgt tctgataatt gcagagttgt ttttgaaggc
     5221 ggttgtggtg catcagcccg tgacctgtgg gcaggcgaca ggtatcactg gtgggataag
     5281 actgtctgat tggtattata aatatataag agagctcaaa cacatgagtt catatatgtg
     5341 aaataaatct gtgagtgtga tcagcagacc agtctaccca gtagcaacag attgcgttgt
     5401 actcacaggg ttatccctga taactggcgt ataacctgcg tacataaaac gtacctgcaa
     5461 tgcacctgaa cagagtgcat taagcatccg cctgagcggt aaacgtatcc gatctggtga
     5521 gtgacagtct gagagcattc tcagtaatcg cccccttgta tcctggaaat tggttaagat
     5581 aacagcgatt agatttggca aggtgtatgc agccagcagc ttaacatctc agctaactgg
     5641 ctggttacct tcaggttgtc gcaatggcat cggacatccg gtgtcggata actaaacgta
     5701 gcgttaaggc gatattgctg agagaccggt taagtggttg cacgggagta atgagtttgg
     5761 ggagggtacc agaatgtcag tctggtgcgg tatgctggca gtatcacgtt gattacagca
     5821 taaagtaacg agagacgaat ggaagttaaa accagagcag ttccgtcgtt gttcatcccg
     5881 ggagacgctg gaaaaagtga tttcccacac acgttataaa cttacccctg cggagctgga
     5941 agcctttaac tctgcggtcg atcaccggct ggcagaactg acaatgaaca aactttacga
     6001 tcgcgtgccg gcttccgtct ggaaatatgt cacctgaatc ttgaatccgc gctacgatga
     6061 ctccggtgtt atctgtgcac cggaagatcg ggaacattaa gcagtataac ggcaacaaag
     6121 aattcgaaat caaaagggaa tggtgtacag aggggcaggc gttcaggcat taccggcctg
     6181 tatcttctga aggggactcg tatttgtcgg ggctgagtat tccgctcgat tcgatcacgg
     6241 attggcggaa tacctgactg tgattgattc aggtaccatt ttgcagggag ccggtgcgtt
     6301 actgtctggc acaacaaacg cagcgggtcg ctctgccccg ccgattcctg ataatccctc
     6361 ctctgtaacc tactgcaaca gtacaaatac actgacgcct gaaacggtaa ccagaatcag
     6421 caccagcagt aacgtcagta aacagagcag acgatcggtc ttccggtaat aaccgaaggg
     6481 gtcgacagcg ccacaatacg ggcaacatac tgtattatga gtaattgcgt taccacagtc
     6541 cctgcacttc tttttgcgta tcacgatcgc tccctctcca cgccttatcc gtaacctgtt
     6601 ttttacatta aaaaaacaac gccgggaatt caatcgtcag tccggcgatg accgttcggg
     6661 ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca cctcaggggc tctgcgcatt
     6721 ttttacgacg tggttattcc gtatgaacca tacggagatt taaccatgac ctacaaatac
     6781 aaccccttct ggcagcaacg tattcgtgag acggtgcggc acgcactgaa tgttcat
//
LOCUS       DAOFSV010000195         6711 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.195, whole genome shotgun sequence.
ACCESSION   DAOFSV010000195 DAOFSV010000000
VERSION     DAOFSV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.195"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..762)
                     /locus_tag="QBH27_004322"
     CDS             complement(<1..762)
                     /locus_tag="QBH27_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HDQ7016557.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGSRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQYDPQRVRAAAYWHAK"
     gene            1050..1673
                     /gene="nfeR"
                     /locus_tag="QBH27_004323"
                     /gene_synonym="yqjI"
     CDS             1050..1673
                     /gene="nfeR"
                     /locus_tag="QBH27_004323"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDQ7016558.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLCVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(1780..3300)
                     /gene="aer"
                     /locus_tag="QBH27_004324"
     CDS             complement(1780..3300)
                     /gene="aer"
                     /locus_tag="QBH27_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDQ7016559.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFIVLAAMLWFVAAPVVTYIFCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSCLTRAVDELNLITQKNAELVEESAQASAMVKHRASRLEDAVTVLH"
     gene            3718..5097
                     /gene="ygjG"
                     /locus_tag="QBH27_004325"
     CDS             3718..5097
                     /gene="ygjG"
                     /locus_tag="QBH27_004325"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDQ7016560.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5139..5471)
                     /locus_tag="QBH27_004326"
     CDS             complement(5139..5471)
                     /locus_tag="QBH27_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDQ7016561.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            5690..6673
                     /gene="ebgR"
                     /locus_tag="QBH27_004327"
     CDS             5690..6673
                     /gene="ebgR"
                     /locus_tag="QBH27_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDQ7016562.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1618 a         1810 c         1692 g         1591 t
ORIGIN      
        1 ctttgcgtgc cagtaagccg ccgcacgtac ccgctgtggg tcatactgtt ccgtttcaaa
       61 gcggcggctt aaattcttaa cgactttgcc ttcgccggtt atccagatga agtaatcatc
      121 ggcagggatt tgcatctgcg ccagacgcgc atctaccgcc tgctcatcat gtgccagcca
      181 ttcgatatta aaaccatcaa ggtgcgcgag ataatcctga caggcgttat cccgcacgct
      241 aaccagcgca ctaacttgcg gtttaacagc aagtttgctc aacgtttcca ggcggcggcg
      301 cagtgcgggc attccggatt catcgcagac atacagctga tacgcgtaat cttccggcac
      361 caccagcgaa ccgcgcggac ctgccaccgt aagtttatcg cccggtttcg cctgcatcgc
      421 ccagccgctg gcgaccccac cgtcgtgaat aaaaaaatca atcgccagtt catggcgtag
      481 ttcgtcatac agcggcgtat agtcacgcga cggtgggcgt gatccttccg gccagacgat
      541 gccctcttcc gttaccgttg gcggcacaaa gtgagcgtca ggttgaggaa agaagagttt
      601 gctgtgatcg tcaaagccgc gcgatgtaaa accgtccagc gcctcgccgc cgaggacaat
      661 gcgctgaaaa ccggcgctga tgcgctcaac gcgtagcaca gtcagttcac ggaagcgcag
      721 atcattgcga acgcgctgtg ggtagcgggg ggtgttattc attgttatcg ccttcgtgat
      781 ggtaatcaga tatatctaaa taaattccac aaatgataat gattgttaat catgataaat
      841 gcaagtgatt tgtagcactg atatatctgt agtctgataa gacgcgcaac ctcttatcaa
      901 gcatcattgc tcaatgccgg atgcggcgtg aacgccttat ccggcctaca tgttgagtaa
      961 acctgcgttt aaaaatcatt tttgtacttg cagtaatgct catatcagat ataaattaga
     1021 tatatctaat taagcaaaag gaggctgata tgagccatca tcacgaaggg tgttgtaaac
     1081 atgaaggcca gccacgccat gagggctgct gcaaaggtga gaagtcagaa cacgagcact
     1141 gcggacacgg tcaccagcat gaacacggtc aatgctgcgg tggtcgccac ggtcgcggcg
     1201 gcggtcgtcg gcaacgtttc tttggtcacg gtgaattgcg tctggtgatt ctggatattc
     1261 tctcgcgcga tgacagccac ggttacgagt tgattaaagc gattgaaaat ctgacccagg
     1321 ggaattacac cccaagcccg ggcgtcatct acccgacgct ggattttctg caggagcagt
     1381 cgctgattac catccgcgaa gaggaaggtg gtaagaagca gattgcgctg accgaacaag
     1441 gcgcgcagtg gctggaagaa aaccgcgaac aggtagagat gattgaagaa cgcatcaaag
     1501 cgcgttgcgt tggcgctgcg ctgcgccaga acccgcaaat gaagcgggcg ctggataatt
     1561 ttaaagcggt gctggattta tgcgtcaacc agagcgatat cactgatgca caaataaaaa
     1621 agatcattgc ggtgatcgac cgcgccgctt ttgatattac ccaactggat taatcaccga
     1681 ttgccggatg cggcgtaccc tacacacccc gtaggcctga taagatgcgc cagcatcgca
     1741 tcaggcattg tgctccaacc gccggatccg gcataccgat taatgcagca ccgtcaccgc
     1801 gtcttccagt cggctggcgc ggtgtttcac catcgccgac gcctgcgcac tctcttccac
     1861 taactcggcg tttttctggg tgatcaggtt aagctcatcc actgcacggg tcaggcagga
     1921 aagcccatcg gcctgttcca gcgttgaatg gctaatctga gctatcaact gggtgacgtt
     1981 ttttacctgc gccacaatat cttccatcgt ccgtccggcg gcgtgtacct gctgcgaacc
     2041 agattgcacc ttatcagcac tggcatcaat cagcttgcga atatcgttgg cagcattggc
     2101 gctgcggctg gctaaatgac gcacttcccc cgccaccact gcaaaacctt tgccctgttc
     2161 gccggcacgc gccgcttcca ccgccgcatt cagggccaga atattggtct gaaacgcaat
     2221 atcgttaatc agcgaagtaa tggtgccaat gcgctgggta ctgtcggcaa tatcgtccat
     2281 tgtcttgatc accgtagtca tcgcctcccc gccctgcact gccgcattac tggcggtgat
     2341 agaaagttta tcggcagccg acgccgtggc agagttctgt ttcaccgacg ccgccatttg
     2401 gttcatggtc gccaccgttt gctgaacgtt atcaactgtc tgctgggtat gttcgttcag
     2461 ttcatcggtg cctttcgcca gcgtctcact gccgtttctg acactggaca cctggcttga
     2521 gacatcatta attagccaac ggcacatcag gccaagctgc cctaccgcgc gtaatgtcag
     2581 ccccagctca tcgctgcgct tcagatgctc aacactatta cgttctccgg tcgccacttt
     2641 cagtgcctgg ctggcgacat tttctatcgg acgtacaatt tgccattcaa aacaggcatt
     2701 tcccaacaac atcactaatg cacagaagat atacgtcacc accggggcag caacaaacca
     2761 gagcatggcc gccagcacga taaacatcag ggtcatcact ccacgcgccc gccagcgaag
     2821 cggtaatgaa ggcagtttac ccagccagcc tttacgcacc accaggcctt tatgaatacg
     2881 cttaccggta cgtccggcat tcagcgcttt gtacagcggc tccaccgccg cgatctcttc
     2941 atccgtcgcc cgggtacgaa tcgacatata gccactgatt tttccctcgc gcaccatcgg
     3001 taccgcattg gcccgcaccc aataatggtc gccatttttg cggcgatttt tcacgatgcc
     3061 gctccagggt tccccttttt tcagggtgaa ccacatatcc gcaaacgccg cttttggcat
     3121 atccgggtga cgcaccatgt tgtgcggttg cccctgtaac tcctgcaagg tatagccgct
     3181 cacctgcaca aaagtgtcat tagcatgagt gatatagctt tgcagatcgg tagtggacat
     3241 cagagtggtg tcgtccgcca gcggggtatt ttgctgggtg acatacggat gagaagacat
     3301 gttcgcgtcc tgtgcaggtt atatggttgt taacttcttg tcagagttta tgtcggcccc
     3361 gctgcggtta tctttaacca attaatttga tttagatcgc aatttgcgat taaagcacaa
     3421 atctaattcc ttgatttaaa atactttcac tctgttacta tacgaaaacg ttaattatct
     3481 tgcccaaaaa tcaggcaatt attgccctga aagcgtgcat ttgcgcagca atcatcaaat
     3541 ccatacccga caaaaaccgt gcaaaataac agcaaatgtt agcaaatagc attaaatatt
     3601 gcgcaaatga taaccacatt tgtgtttatc ccgattttcg cgatcgcagc cggagtggcg
     3661 caatccctgc aatacttaaa tcggtatcat gtgatacgcg agcctccgga gcatattttg
     3721 aacaggttac cttcgagcgc atcagcttta gcgtgcagcg cccacgccct gaatctcatt
     3781 gaaaagcgaa cgttggatca tgaggagatg aaagcactta accgggaggt gattgaatac
     3841 ttcaaagagc atgtcaatcc ggggttttta gagtatcgca aatctgttac cgccggcggg
     3901 gattacggag ccgtagagtg gcaagcggga ggtttaaata cgcttgtcga cacccaggga
     3961 caggagttta tcgactgcct gggaggtttt ggaattttca acgtggggca ccgtaatcca
     4021 gttgtggttt ccgccgtaca gaatcaactt gcgaaacaac cgcttcacag ccaggagctg
     4081 cttgatccgt tacgggcgat gctggcgaaa acccttgcgg cgctaacgcc cggtaaactg
     4141 aaatacagct tcttctgtaa tagcggcacc gaatccgttg aagcggcgct gaagctggcg
     4201 aaggcttatc agtcaccgcg cggcaagttt acttttattg ccaccagcgg cgcgttccac
     4261 ggtaaatcac ttggcgcgct gtcggccacg gcgaaatcga ccttccgcaa accgtttatg
     4321 ccactgctgc cagggtttcg tcatgtaccg tttggcaata tcgaagccat gcgcacggct
     4381 cttaacgagt gcaaaaaaac cggtgatgat gttgctgcgg tgatcctcga accgattcag
     4441 ggcgaaggtg gcgtaattct gccgccgcca ggctatctca ccgccgtgcg taagctgtgc
     4501 gatgagttcg gcgcactgat gatcctcgat gaagtacaaa cgggcatggg gcgcacgggc
     4561 aagatgttcg cctgcgagca tgagaacgta cagccggata tcctttgtct ggcaaaagcg
     4621 ctcggcggcg gcgtgatgcc gattggcgca accatcgcca ctgaagaggt gttctcagtc
     4681 ctgttcgaca atccgttcct gcacactacc acctttggcg gcaacccgct ggcctgtgcg
     4741 gcggcgctgg cgaccatcaa tgtgttgctg gagcagaact taccggctca ggctgaacaa
     4801 aaaggcgata tgttgctgga cggtttccgt caactggcgc gggaatatcc cgatctggta
     4861 caggaagcgc gtggtaaagg gatgttgatg gcgattgagt ttgttgataa cgaaatcggc
     4921 tataactttg ccagcgagat gttccgccag cgcgtactgg tggccggaac actcaacaac
     4981 gctaaaacga tccgcattga accgccactg acactgacca ttgaacagtg tgaactggtg
     5041 atcaaagcgg cgcgtaaggc gctggcggcc atgcgagtca gtgtcgaaga agcgtaatat
     5101 caaatcggat ggcgatacga cgtcgccatc cgatcttttt atacgatacg tacgcccgca
     5161 ggcatcatcc gctccggcgt taacagcacg ctttcactgc cgtcatcggt ttccgcacac
     5221 aacagcatgc attccgacgt ttcaccgcgc attttcgctt tttgcagatt gcataagacc
     5281 accactgttt ttcccatcag ctcttcttcg ctgtagtacg gcaccagact ggtcacggtt
     5341 tgcagcgttt tttcccccac atcaacctgt acgatgtaca gcttgtcggc gttttcatgg
     5401 cgtttcactt ccacaatctt tccgacgcgc atttccagac gtgcaaaatc agcgtaagcc
     5461 acggtttcca ttgtttcctc ccttcaagta aaattttact aaactatagc aaaagttttt
     5521 ctcaatcctg taggctaaaa atggagaatg caggcgtgat cacattcgtg agccgctgtg
     5581 ttaccgtcac agcgtcaaag aaacgcgctt tatttactga aaacaggtga cccgataagc
     5641 acttcctcta caatgggggc gcacatcagg gaaagtaaaa aaggtaaaca tggcaacact
     5701 aaaagacatc gcaatcgaag ctggcgtatc cctggcgaca gtatccaggg tcttgaatga
     5761 cgatccgaca ttgaatgtga aagaagagac gaaacatcgc attctcgaga tcgccgaaaa
     5821 gctggagtac aagaccagta gcgcccgtaa actccagaca ggcgcagtca accaacacca
     5881 tattctggct atctacagct accaacagga gttggagatc aacgatcctt actatctggc
     5941 gatccgccac ggcattgaaa ctcagtgcga aaagctggcg atcgaactga ccaactgtta
     6001 tgaacacaac ggcttaccag acattaaaaa cgtcaccggt attttaattg tcggcaaacc
     6061 cacgtccgcc ctgcgcgccg ccgcctgtgc gttgaccgac aatatctgtt ttatcgactt
     6121 tcacgaaccc ggcagcggtt acgatgcggt ggatatcgat ctggcacgta tcagtaaaga
     6181 aatcatcgat ttctatatca atcagggcgt taatcgtatt ggttttattg gcggtgaaga
     6241 cttacccggc aaggcggata ttcgtgaggt cgcctttgcg gaatatggtc gtctgaaaca
     6301 cgtggtacgc gaagaggata tctggcgcgg cggtttttcc agttcgtcgg gctatgaact
     6361 ggcaaaacag atgctggcgc gggaagacta tccgaaggca ctgtttgttg cttccgattc
     6421 cattgctatc ggcgtactgc gggcaattca tgaacgtggc ctgaacatcc cacaggatat
     6481 ttcgcttatc agcgttaacg atatccccac cgcgcgattt acctttccgc cgctctccac
     6541 cgtgcgcatc cattccgaga tgatgggaag tcagggcgtt aacctggtgt atgaaaaagc
     6601 ccgcgatggt cgcgcgctgc cgctgttggt cttcgttccc agcaaattaa aactgcgcgg
     6661 cacgacccgt taaatcccct tacacactgt ccggcaatcg tttttgccgg a
//
LOCUS       DAOFSV010000196         6653 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.196, whole genome shotgun sequence.
ACCESSION   DAOFSV010000196 DAOFSV010000000
VERSION     DAOFSV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.196"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            374..1249
                     /locus_tag="QBH27_004328"
     CDS             374..1249
                     /locus_tag="QBH27_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDQ7016563.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATAQHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQILALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1257..2033
                     /locus_tag="QBH27_004329"
     CDS             1257..2033
                     /locus_tag="QBH27_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDQ7016564.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2202..4463
                     /locus_tag="QBH27_004330"
     CDS             2202..4463
                     /locus_tag="QBH27_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ7016565.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSLPEVIGNGW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTH
                     FGAGDITELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4532..5707
                     /gene="senB"
                     /locus_tag="QBH27_004331"
     CDS             4532..5707
                     /gene="senB"
                     /locus_tag="QBH27_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HDQ7016566.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6230..6535)
                     /locus_tag="QBH27_004332"
                     /pseudo
     CDS             complement(6230..6535)
                     /locus_tag="QBH27_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1885 a         1567 c         1539 g         1662 t
ORIGIN      
        1 ttaacgtacg ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc
       61 gtattccccc cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
      121 gcaggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
      181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac ccataatatc
      241 ggctggtaaa catcactcgc tagtgcattc tttttgttgg taatgataat catttacatt
      301 cattctttga tgttttatca taattaatgt tttgtcaata aatatatgga aacatattgt
      361 ttcagatggg aatatgagaa agttcatcct tatttctatg ataacgttag ctagtgttag
      421 tctgggagcc tgcagacaga atgtcaccat aaagcaggat gcgccagggc agaaagcttt
      481 cctgactgac gggcagattt acgatttgca cagtggcaaa attatttcta gcagcgagct
      541 tttggcggat cttgctaccg ctcaacatct tattattggt gaaaaacacg ataatgccga
      601 gcatcatcag attgagcttt ggcttattca aaatttgctc atccagcgac cacaggggag
      661 tgtgttgctt gagatgttga cgtcagagca acagccccga gttaaccaag ttaaatgttg
      721 gctgaaagat aatcctgtgg ttcgagatag ccgtgttcag gaactactta actggcaaaa
      781 agggtggtcc tgggagatgt atggcgacat cgtcatgcag ctattacgtg ggccttaccc
      841 gctgctcaat gccaacattg gccgggagca gatattggca ctgtacaaaa aaaacgaatt
      901 tcctaaagga aaaaaatcca ctgcacctgt ggtacaagaa gctctgcgtg aaaccatcat
      961 atctatgcat gaaggcaacc ttgaaagcca gcaactgacg tcaatgctct ctattcagca
     1021 gcagcgggat cgctacatgg cccgccagct tctgagtgcc ccggtaccgt cgttgttgat
     1081 tgcaggtggt tatcacgcgt caaaaagcat gggcgtaccg ttacacatgg aagatcttgc
     1141 caccggcacc catccagtgg tgttgatgct tgcagaaaaa ggaatgaaca tcacagtaga
     1201 tcatgctgac tacgtgtggt ttgtagcgcc agatacaaca aaaaggtaaa ccatggatga
     1261 tgaatattct ccacttctca cagtcggtaa aatggagcag ctggtttatc tgttctctac
     1321 tgcttcatgg actgattttc cttgcgttta tctggcgttt ttccgaagtt cagcccgcta
     1381 tgtctcctgc accggcgatc atgctgcaat gggcagaaat tatagaggct ccttcttctc
     1441 cactttcgct tccggtggga atcgctcaac aggagtccgc agtgacagaa gaaaagcaac
     1501 aaactgagga cagacaacaa cgtcctgtca ctgaagacag tgatgcaacc attgaaataa
     1561 ctaggaagaa aaagtcatcc gatggagaaa agaaaaagac acgtccacct cgtaagataa
     1621 aggctcaaac cagcgacagc aaccccacag ccgtttccag taatgctgca cctcaggctc
     1681 tggtcgaatc gtcccgtatt gctgcgcctt ttaacagtga ctcaacaaag cgagataatt
     1741 ctgaggcatc ttgggaaagc agagtaaaag gccacctaaa ccgttataaa cgttaccctg
     1801 gcgatgccag aaaacgtgca cgaaccggca cagccgtcgt gacttttacc gtaaacacgg
     1861 aaggcactat tgtcagtagt ttcttggaaa tctcttcagg aaccttttct cttgaccgcg
     1921 aagctattgc tgttttagaa cgcgctcaac cacttcccaa accaccgcct gaaatattgg
     1981 agggaggatt atttaaagta aaaatgccga ttacctttaa gttgaaggag taaagggata
     2041 attggccgtt caagttcatt taactgaaat ttaattttca tttttatgct gaacatttta
     2101 tcaaactttt ccactgtcaa aaaaatcaaa aagacagtag aacataatta ataaacaaat
     2161 agttatactt atctttatta atattatacc caaggaaatt tatgaacgtt ataaaactgg
     2221 ctatcggctc aggcatatta ttgctcagct gcggtgctta ctcacaatcc atcagtgaaa
     2281 aaactaattc cgacaaaaaa ggagcggcag aattcagtcc gctcagcgtt tctgtcggga
     2341 agacgaccag tgagcaggaa gctctcgaga aaacaggcgc gaccagttcc cggacaacgg
     2401 acaaaaacct gcaatcactt gacgcaacag tgcgtagtat gcctggtact tatactcaaa
     2461 tagatcctgg tcagggagca atcagtgtga atattcgagg catgagcgga tttggtcgtg
     2521 taaacactat ggtcgatggt attacccaga gtttttacgg aacctctacc tccggaacaa
     2581 cgacgcatgg ttcaactaac aatatggctg gcgtacttat agatcctaac ttactggtag
     2641 cagttgatgt tacacgcggt gacagcagtg gctctgaagg gatcaacgcc cttgccggta
     2701 gtgcaaatat gcgtactatt ggcgttgacg atgtaatatt taacggtaat acatatggcc
     2761 ttcgttcacg tttctctgtc ggtagtaatg ggctgggacg cagcggaatg atcgcccttg
     2821 gtggaaaaag cgacgctttt acggatacgg gaagcattgg cgttatggct gctgtgagcg
     2881 gcagttctgt gtactctaat ttctcaaatg gttctggaat taacagcaaa gagtttggtt
     2941 atgataaata tatgaagcag aaccccaaat cccaactgta taaaatggat atcagaccag
     3001 acgaatttaa cagcttcgaa ctttccgctc gaacctatga aaataaattt acacgtcgtg
     3061 atataaccag tgacgactat tacattaaat atcattacac ccctttttct gaattaattg
     3121 actttaacgt aacggccagt accagtcgcg gtaatcaaaa gtatcgtgat ggctcgctgt
     3181 atactttcta caaaacctca gcgcaaaatc gttctgacgc gctggatatc aacaatacca
     3241 gccggttcac tgtcgcggac aatgacctgg agtttatgct gggcagcaaa ctgatgcgta
     3301 cccgctatga ccggaccatt cactcagcgg cgggcgaccc gaaagcgaat caggaatcga
     3361 tcgagaacaa tccgttcgca ccctccggcc agcaggatat ttcagcgctg tataccgggc
     3421 tgaaggttac gcgcggcatc tgggaggcag atttcaatct caactacaca cgtaacagga
     3481 tcacagggta caagcccgcc tgcgattcac gcgttatctg cgtgccacag ggtagctacg
     3541 atattgacga taaagagggt ggcttcaacc cttcagttca gctttctgct caggtaacac
     3601 catggcttca gccgttcatt ggctacagca aatccatgcg cgccccgaac atccaggaga
     3661 tgttcttctc taattcagga ggcgcatcca tgaacccatt cctgaagcct gaacgtgcag
     3721 aaacctggca ggcgggtttt aacattgata ccagagattt actggtcgaa caggatgccc
     3781 tgcgctttaa ggctctggcg taccgcagca ggatccagaa ctacatctac agcgagtctt
     3841 atctggtttg ttctggaggt cgtaaatgca gtctgcctga ggtgattggc aatggctggg
     3901 agggcattag cgatgaatac agcgacaata tgtacatcta cgttaactcg gcaagcgacg
     3961 ttatcgcaaa gggcttcgaa ctggagatgg attatgatgc aggttttgct tttggccgac
     4021 tctctttcag ccagcagcaa acagaccagc caacctccat cgccagcacc cactttggcg
     4081 caggggatat aaccgaactg cccagaaaat acatgacgct ggatactggt gttcgcttct
     4141 tcgataacgc gttgaccctg ggcactatca taaaatacac aggcaaggct cgtcgcctgt
     4201 cgcctgattt tgagcaggac gaacataccg gcgcaataat caaacaggat ttgccgcaga
     4261 tcccaacgat tatcgatctc tatggtactt acgagtacaa ccgcaacctg acactgaaac
     4321 tttcggtaca aaacctgatg aacagagatt attcggaggc gctgaataag ctcaacatga
     4381 tgccaggtct tggtgacgag acccacccag ccaattccgc gcgtggcaga acatggatat
     4441 ttggcgggga cattcgtttc tgatgacaat ttccgctagg ttcagacaat acgttttctc
     4501 tcttatgtca attttattgc aggaacgaaa aatgaatatt ttcactttat ccaaagcacc
     4561 gctatacctg ttaatttcac tatttttacc cacgatggcc atggctatcg atccacctga
     4621 acgcgaactt tcgcgatttg ccctgaaaac gaattacctt cagtcccctg atgaaggcgt
     4681 ctatgaactg gcgtttgata atgccagtaa aaaggtgttt gcagcagtca ccgatcgtgt
     4741 aaatcgtgaa gccaataaag gctatctgta ttcgtttaat tcagattcgc tgaaagtcga
     4801 aaataaatac acgatgccat accgggcatt ttcgctggcg ataaatcagg ataaacatca
     4861 gctctatatc ggacacaccc agtcagcgtc cctgcgtatc agtatgtttg acaccccaac
     4921 cggcaaactg gtaagaacca gcgacaggtt aagttttaaa gcggcaaacg ctgcagattc
     4981 gcgttttgag cattttcgcc atatggttta cagccaggat tccgataccc tgtttgtgag
     5041 ttatagcaat atgctgaaaa cggccgaggg catgaagcct ctgcataagc tgttaatgct
     5101 cgacgggacg acgcttgcct taaaaggcga ggttaaggat gcttacaaag gtacagcgta
     5161 tggtctgacg atggatgaaa aaacacagaa aatctacgtt ggcggaagag attacatcaa
     5221 cgaaattgat gcgaaaaatc agacgctgct gcgtaccatc ccgttgaaag atccgagacc
     5281 acaaatcaca agtgtgcaga atctggcggt ggactccgct tctgaccgtg cctttgtggt
     5341 ggtattcgac catgacgatc gttccggtac aaaagatgga ctctatattt ttgacttacg
     5401 cgacggtaaa cagcttggct atgtgcacac aggagccgga gctaacgcgg tgaaatacaa
     5461 tccgaaatat aacgaactgt atgtcaccaa cttcactagc ggcaccatca gcgtagtgga
     5521 tgccaccaaa tacagcatca cccgtgaatt taacatgccg gtctacccaa accagatggt
     5581 gttgtcggac gatatggata ccctttacat tggcatcaaa gaaggcttta accgcgattg
     5641 ggatcctgat gtgtttgtgg aaggagctaa agaacgtatt ctgagcattg atttgaaaaa
     5701 gtcgtgatcg gggggaaaaa acatgggata aaaactccgt tattccacta aactggccgg
     5761 actaggaaaa gcaaacatct ctgtcctgcc ggtacgcctg ttacgccgct ccatgaggct
     5821 gaggaatctc cagaaaacga gacaggcaca gcattgtacg atcaactgat ttgtgccaat
     5881 aattaaatga aatcacagct atgcctgttc atggggttct aggccggaac cccagatttt
     5941 tccgattgtt tgttttctaa ctgattattc agtagtttta gagggctgac gctcagtgga
     6001 acgaaaactc acgttaagag gttttgacta tgataaaagt caaagtaggg gtgagtttgg
     6061 atatggaaat tattgtacta taaggagagc atgaaaaacc ctggggcagt cactgaatga
     6121 gggaccagca gcggtaaaat aaccagcata atttctgcat ggtcgatttt tataccgtga
     6181 caggctgttt tttatacttg caccgattcc cgggcacact tagcctgtgt caggccggca
     6241 tatacctcag cagactgata tcccgcgcgt ttttcttcag ccctgataca ttcagccccg
     6301 caatggcccg gcggtatacc atccggccgc cgcacagcac gcattcgaac gggtcacgac
     6361 tcaggaactg tttcaccatt tgtgcatagc acactttcgc cactgcttcc ggtttatcca
     6421 tccccagagc acggtacacc tgcggcagct tctctccaca cacccggttg gcaaggaacc
     6481 cgaagtacct caccatctta aaaaactgaa ccgccccggg aatcctggag actaaacttc
     6541 ctgagaaaga ggtaaacagg atgactaaaa atactcgttt ttcccccgaa gtccgtcaac
     6601 gggcagtccg tatggttctg gaaagtcagg gcgaatatga ctcacaatgg gcg
//
LOCUS       DAOFSV010000197         6553 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.197, whole genome shotgun sequence.
ACCESSION   DAOFSV010000197 DAOFSV010000000
VERSION     DAOFSV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.197"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            10..1572
                     /gene="trpE"
                     /locus_tag="QBH27_004333"
     CDS             10..1572
                     /gene="trpE"
                     /locus_tag="QBH27_004333"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDQ7016567.1"
                     /translation="MQTQKPTLELLTCVGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGVATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            1572..3167
                     /gene="trpD"
                     /locus_tag="QBH27_004334"
     CDS             1572..3167
                     /gene="trpD"
                     /locus_tag="QBH27_004334"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDQ7016568.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEQTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRCVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            3171..4529
                     /gene="trpCF"
                     /locus_tag="QBH27_004335"
     CDS             3171..4529
                     /gene="trpCF"
                     /locus_tag="QBH27_004335"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDQ7016569.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            4541..5734
                     /gene="trpB"
                     /locus_tag="QBH27_004336"
     CDS             4541..5734
                     /gene="trpB"
                     /locus_tag="QBH27_004336"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDQ7016570.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            5734..6540
                     /gene="trpA"
                     /locus_tag="QBH27_004337"
     CDS             5734..6540
                     /gene="trpA"
                     /locus_tag="QBH27_004337"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDQ7016571.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPVGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKATTRR"
BASE COUNT         1506 a         1750 c         1808 g         1489 t
ORIGIN      
        1 agaataacaa tgcaaacaca aaaaccgact ctcgaactgc taacctgcgt aggcgcttat
       61 cgcgacaacc caaccgcgct ttttcaccag ttgtgtgggg atcgtccggc aacgctgctg
      121 ctggaatccg cagatatcga cagcaaagat gatttaaaaa gcctgctact ggtagacagt
      181 gcgctgcgca ttacagcatt aggtgacacc gtcacaatcc aggcactttc cggcaatggc
      241 gaagccctgc tggcactact ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca
      301 ccaaactgcc gcgtactgcg cttcccgcct gtcagtccac tgctggatga agacgcccgc
      361 ttatgctccc tttcggtttt tgacgcattc cgcttattac agaatctgtt gaatgtaccg
      421 aaggaagaac gagaagcaat gttcttcggc ggcctgttct cttatgacct tgtggcggga
      481 tttgaagatt taccgcaact gtcagcggaa aataactgcc ctgatttctg tttttatctc
      541 gctgaaacgc tgatggtgat tgaccatcag aaaaaaagca cccgtattca ggccagcctg
      601 tttgctccga atgaagaaga aaaacaacgt ctcactgctc gccttaacga actacgtcag
      661 caactgaccg aagccgcgcc gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa
      721 tgtaaccaga gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc
      781 gccggagaaa ttttccaggt ggtgccatct cgccgtttct ctctgccctg cccgtcaccg
      841 ctggcggcct attacgtgct gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag
      901 gataatgatt tcaccctgtt tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc
      961 agccgccaga ttgagattta cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat
     1021 ggttcactgg atagagacct cgacagccgc atcgaactgg agatgcgtac cgatcataaa
     1081 gagctttctg aacatctgat gctggtggat ctcgcccgta atgacctggc gcgtatttgc
     1141 acccccggca gccgctacgt cgccgatctc accaaagttg accgttactc ttacgtgatg
     1201 cacctggtct cccgcgttgt tggtgagctg cgccacgatc tcgacgccct gcacgcttac
     1261 cgcgcctgta tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc tatgcagtta
     1321 attgccgaag cagaaggtcg tcgccgcggc agctacggcg gcgcggtagg ttattttacc
     1381 gcgcatggcg atctcgacac ctgcattgtg atccgctcgg cgctggtaga gaacggagtc
     1441 gccaccgtgc aagccggtgc tggcgtggta cttgattctg ttccgcagtc ggaagccgac
     1501 gaaacccgta ataaagcccg cgctgtactg cgcgctattg ccaccgcgca tcatgcacag
     1561 gagacgttct aatggctgac attctgctgc tcgataatat cgactctttt acttacaacc
     1621 tggcagatca gttgcgcagc aatggtcata acgtggtgat ttaccgcaac catattccgg
     1681 cgcagacctt aattgaacgc ctggcgacga tgagcaatcc ggtgctgatg ctttctcctg
     1741 gccccggtgt gccgagcgaa gccggttgta tgccggaact cctcacccgc ttgcgtggca
     1801 agctgccaat tattggcatt tgcctcggac atcaggcgat tgtcgaagct tacgggggct
     1861 atgtcggtca ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa catgacggtc
     1921 aggcgatgtt tgccggatta acaaacccgc tgccagtggc gcgttatcac tcgctggttg
     1981 gcagtaatat tccggccggt ttaaccatca acgcccattt taatggcatg gtgatggcgg
     2041 tgcgccacga tgcggatcgc gtttgtggat tccagttcca tccggaatcc attcttacta
     2101 cccagggcgc tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa ctagagcaaa
     2161 ccaacacgct gcaaccgatt ctggaaaaac tgtatcaggc acagacgctt agccaacaag
     2221 aaagccacca gctgttttca gcggtggtac gtggcgagct gaagccggaa caactggcgg
     2281 cggcgctggt gagcatgaaa attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa
     2341 ccgcgctact ggaaaacgcc gcgccgttcc cgcgcccgga ttatctgttt gccgatatcg
     2401 tcggtactgg cggtgacggc agcaacagca tcaatatttc taccgccagt gcgtttgtcg
     2461 ccgcggcctg cgggctgaaa gtggcgaaac acggcaaccg ttgcgtctcc agtaaatccg
     2521 gctcgtcgga tctgctggcg gcgttcggta ttaatcttga tatgaacgcc gataaatcgc
     2581 gccaggcgct ggatgaatta ggcgtctgtt tcctctttgc accgaaatat cacaccggat
     2641 tccgccatgc aatgccggtt cgccagcaac tgaaaacccg cacactgttc aatgtgctgg
     2701 ggccattaat taacccggcg catcctccgc tggcgttaat tggtgtctac agcccggagc
     2761 tggtgctgcc gattgccgaa accttgcgcg tgctggggta tcaacgcgcg gcagtggtgc
     2821 acagcggcgg gatggatgaa gtttcattac acgcgccgac aatcgttgcc gaactgcatg
     2881 acggcgaaat taagagctat caattgaccg ctgaagattt tggcctgaca ccctaccacc
     2941 aggagcaact ggcaggcgga acaccggaag aaaaccgtga cattttaaca cgcttgttac
     3001 aaggtaaagg cgacgccgct cacgaagcag ccgtcgcggc gaatgtcgcc atgttaatgc
     3061 gcctgcatgg ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca
     3121 gtggttccgc ttacgacaga gtcaccgcac tggcggcacg agggtaaata atgcaaaccg
     3181 ttttagcgaa aatcgtcgca gacaaggcga tttgggtaga agcccgcaaa cagcagcaac
     3241 cgctggccag ttttcagaat gagattcagc cgagcacgcg acatttttat gatgcgctac
     3301 agggtgcacg cacggcgttt attctggagt gcaaaaaagc gtcgccgtca aaaggcgtga
     3361 tccgtgatga tttcgatccg gcacgcattg ctgccattta taaacattac gcttcggcaa
     3421 tttcagtgct gactgatgag aaatattttc aggggagctt tgatttcctc cccatcgtca
     3481 gccaaatcgc cccgcagccg attttatgta aagacttcat tatcgatcct taccagatct
     3541 atctggcgcg ctattaccag gccgatgcct gcttattaat gctttcagta ctggatgacg
     3601 aacaatatcg ccagcttgca gccgtcgccc acagtctgga gatgggtgtg ctgaccgaag
     3661 tcagtaatga agaggaactg gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca
     3721 acaaccgcga tctgcgcgat ttgtcgattg atctcaaccg tacccgcgag cttgcaccga
     3781 aactggggca caacgtgacg gtaatcagcg aatccggcat caatacttac gctcaggtgc
     3841 gcgagttaag ccacttcgct aacggctttc tgattggttc ggcgttgatg gcccatgacg
     3901 atttgaacgc cgctgtgcgt cgggtgttgc tgggtgagaa taaagtatgt ggcctgacac
     3961 gtgggcaaga tgctaaagca gcttatgacg cgggcgcgat ttacggtggg ttgatttttg
     4021 ttgcgacatc accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac
     4081 cgttgcagta tgttggcgtg ttccgcaatc acgatattgc cgatgtggtg gacaaagcta
     4141 aggtgttatc gctggcggca gtgcaactgc atggtaatga agatcagctg tatatcgaca
     4201 ctctgcgtga ggctctgcca gcacacgtcg ccatctggaa ggctttaagt gtcggtgaaa
     4261 ctcttcccgc gcgcgatttt cagcacatcg ataaatatgt attcgacaac ggtcagggcg
     4321 ggagcggaca acgtttcgac tggtcactat taaatggtca atcgcttggc aacgttctgc
     4381 tggcgggggg cttaggcgca gataactgcg tggaagcggc acaaaccggc tgcgccgggc
     4441 ttgattttaa ttctgctgta gagtcgcaac cgggtatcaa agacgcacgt cttttggcct
     4501 cggttttcca gacgctgcgc gcatattaag gaaaggaaca atgacaacat tacttaaccc
     4561 ctattttggt gagtttggcg gcatgtacgt gccacaaatc ctgatgcccg ctctgcgcca
     4621 gctggaagaa gcttttgtca gcgcgcaaaa agatcctgaa tttcaggctc agttcaacga
     4681 cctgctgaaa aactatgccg ggcgtccaac cgcgctgacc aaatgccaga acattacagc
     4741 cgggacgaac accacgctgt atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa
     4801 aactaaccag gtactcggtc aggctttact ggcgaagcgg atgggtaaaa ctgaaattat
     4861 tgccgaaacc ggtgccggtc agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg
     4921 cctgaaatgc cgtatttata tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt
     4981 ccggatgcgc ttaatgggtg cggaagtgat cccggtgcat agcggttccg cgaccctgaa
     5041 agatgcctgt aatgaggcgc tacgcgactg gtccggcagt tatgaaaccg cgcactatat
     5101 gctgggtacc gcagctggcc cgcatcctta cccgaccatt gtgcgtgagt ttcagcggat
     5161 gattggcgaa gaaacgaaag cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt
     5221 tatcgcctgt gttggcggtg gttcgaatgc catcggtatg tttgcagatt tcatcaacga
     5281 aaccgacgtc ggcctgattg gtgtggagcc tggcggtcac ggtatcgaaa ctggcgagca
     5341 cggcgcaccg ttaaaacatg gtcgcgtggg catctatttc ggtatgaaag cgccgatgat
     5401 gcaaaccgaa gacgggcaaa ttgaagagtc ttactccatt tctgccgggc tggatttccc
     5461 gtccgtcggc ccgcaacatg cgtatctcaa cagcactgga cgcgctgatt acgtgtctat
     5521 taccgacgat gaagccctgg aagcctttaa aacgctttgc ctgcatgaag ggatcatccc
     5581 ggcgctggaa tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg aaaatccgga
     5641 aaaagagcag ctactggtgg ttaacctttc cggtcgcggc gataaagaca tcttcaccgt
     5701 tcacgatatt ttgaaagcac gaggggaaat ctgatggaac gctacgaatc tctgtttgcc
     5761 cagttgaagg agcgcaaaga aggcgcattc gttcctttcg tcaccctcgg tgatccgggc
     5821 attgagcagt cgctgaaaat tatcgatacg ctaattgaag ccggtgctga cgcgctggag
     5881 ttaggcatcc ccttctccga cccactggcg gatggcccga cgattcaaaa cgccacactg
     5941 cgtgcctttg cggcgggtgt gaccccggcg cagtgctttg agatgctggc actgattcgt
     6001 caaaaacatc cgaccattcc tgttggcctg ctgatgtacg ccaacctggt gtttaacaaa
     6061 ggcattgatg agttttacgc ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc
     6121 gatgtgccag ttgaagagtc cgcgcccttc cgccaggccg cgttgcgtca taatgtcgca
     6181 cctatcttta tttgcccgcc gaatgccgac gatgatttgc tgcgccagat agcctcctac
     6241 ggtcgtggat acacctattt attgtcacgt gcgggcgtga ccggcgcaga aaaccgcgcc
     6301 gcgttacccc tcaatcatct ggttgcgaag ctgaaagagt acaacgctgc acctccattg
     6361 cagggatttg gtatttccgc ccctgatcag gtaaaagcag cgattgatgc aggagctgcg
     6421 ggcgcgattt ctggttcggc catcgttaaa atcatcgagc aacatattaa tgagccagag
     6481 aaaatgctgg cggcactgaa agcttttgta caaccgatga aagccactac acgacgttaa
     6541 tttgagacaa acc
//
LOCUS       DAOFSV010000198         6488 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.198, whole genome shotgun sequence.
ACCESSION   DAOFSV010000198 DAOFSV010000000
VERSION     DAOFSV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.198"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(223..417)
                     /locus_tag="QBH27_004338"
     CDS             complement(223..417)
                     /locus_tag="QBH27_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016572.1"
                     /translation="MKKLLLAVAFFTLAGCTHSTYTEATRADGSSIKHVMIAPGTKIT
                     TANGGCIDSTGAEASCLGGK"
     gene            complement(425..904)
                     /locus_tag="QBH27_004339"
     CDS             complement(425..904)
                     /locus_tag="QBH27_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HDQ7016573.1"
                     /translation="MTDPKWLIEARKNLGIREMKGKQHAAEIVQYWKDIKRGGIKDDE
                     TPWCAAFTGAMLERAGIRSTRFESANSYLDWGNELKEPAYGCIAILSRSGGGHVGFVV
                     GKNAAGDLMILGGNQADEVNIKAFPRSRVTGYRWPAGQTDVPQSLPLVNAEKSISEA"
     gene            complement(904..1176)
                     /locus_tag="QBH27_004340"
     CDS             complement(904..1176)
                     /locus_tag="QBH27_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDQ7016574.1"
                     /translation="MTLHSVLINANAIICLMLALRLMFFQKTGRYRFFISLTAYLAIL
                     SAAWIALRIFYGKYTQVDPAEFFLNLTICIAVWRARGNISKITGDR"
     gene            complement(1176..1559)
                     /locus_tag="QBH27_004341"
     CDS             complement(1176..1559)
                     /locus_tag="QBH27_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016575.1"
                     /translation="MSEPLTAGVAAGSAGVTFAALFPEATPAVMICALAGAALYVLSS
                     GQHRFWKQVIFALISFVGGVYCAETASAIITGILNAVLSHLNPPVTVKVSPAIGALVA
                     SVISVTSLLRIMSQARLWKSDKGMK"
     gene            complement(1672..2343)
                     /locus_tag="QBH27_004342"
     CDS             complement(1672..2343)
                     /locus_tag="QBH27_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ7016576.1"
                     /translation="MSNEYLLEYTRIKLRAALRDLSGGSKGQLEALCEHPPADKNAYP
                     RKHIHRVQLEDRTVDALVTPVYALESFSRRRPAPPMSDFEFADSSWRRSVNSLDASQQ
                     AWLRYCYGGNLAFKHQTAICEAVWSRYKGNIPASTQRKVVKRLLSLVWLSVQAVAAAN
                     KREDFKEMAGSTLAGMLSVSRSTWCETYSLHWVGMKEAVRALDEVALLATLHHYQNHL
                     DDVCV"
     gene            complement(2343..2636)
                     /locus_tag="QBH27_004343"
     CDS             complement(2343..2636)
                     /locus_tag="QBH27_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDQ7016577.1"
                     /translation="MKKIDLRKAARSRACTVRIPGVCNHNPETSVLAHYRLAGTCGTA
                     IKPHDMQGAIACSACHDAIDGRTKTDYEHDSLLLMHAEGVFRTLAIWRDEEFI"
     gene            complement(2633..3229)
                     /locus_tag="QBH27_004344"
     CDS             complement(2633..3229)
                     /locus_tag="QBH27_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDQ7016578.1"
                     /translation="MAQHSFIRVSGGSLIPATPDTQRWLTERVKPGAVVYADFKQARN
                     PAFHRKFFSLLNLGFDYWHPSGGAISPAERELVHGYVKLLAYYGGHGDVMAELADQYL
                     LDESEKRAGSISAVKSFEAFRAWAIMEAGFYDVHQMPDGSLMRVPRSISFAAMDDLEF
                     GQLYSAVLDVLWNYILFRTFASQEAAENAAAQLLDYTS"
     gene            complement(3307..3486)
                     /locus_tag="QBH27_004345"
     CDS             complement(3307..3486)
                     /locus_tag="QBH27_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016579.1"
                     /translation="MNIQESIGELPETCRAVIKRKDGHIVGVRVLTDDERIASLMAFL
                     ELAEIAGYTITPPDA"
     gene            complement(3638..4279)
                     /locus_tag="QBH27_004346"
     CDS             complement(3638..4279)
                     /locus_tag="QBH27_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016580.1"
                     /translation="MKTFYEDWPETFVSRLDMLRALDDRGSTRRLYLERTGAIFDALA
                     EEIRTVVAGHPEIDVSELDIGPLYRYYKRGEKGNPLADLLIELAPPTCERVRISPEVY
                     IIPYLFFALLIAQGADNDARDFFNMMMRPLIIAYRFKQLARYLGTKGGGRPQHRLKSE
                     AIELADRFFTENPTAPLSRGVQYISGIFVAKYSDPPAASTIRKWLIPIYRSDK"
     gene            4523..4756
                     /locus_tag="QBH27_004347"
     CDS             4523..4756
                     /locus_tag="QBH27_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016581.1"
                     /translation="MGTLGNQPCRSRYEFDDFNLANRAAALTKLAKETGVSVDTLIKL
                     EIAMQLNRLNSILIENGDKMDENLAGIGEILQK"
     gene            5155..5643
                     /locus_tag="QBH27_004348"
     CDS             5155..5643
                     /locus_tag="QBH27_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDQ7016582.1"
                     /translation="MATNPREHARRVLDTFWCGRGFPVDPAKIAHDMGLDVFITDLPG
                     KVSGALIKQKDQDPAIFLNSDDNKVRQRFSCGHELGHYIARQASHSDEYEYVDLRGET
                     ASAGTHPDEIFANKFAAELLMPVDEVRKLHADGQPSYIMAHYFGVSDDAMTYRLKNLR
                     LG"
     gene            5653..6258
                     /locus_tag="QBH27_004349"
     CDS             5653..6258
                     /locus_tag="QBH27_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016583.1"
                     /translation="MPSDDDFNSRFKQEVARRLKDSLNLHRQLAEEVEPEDDETPVQS
                     PDEASPQPKVSHAPKKTAVRIRGIARKRAAEARKMAVAASSKRIPTATPSQQVTRTES
                     KILTEFTSVKQVNSLDTEHERKQLNNKDFEAEIALKKRYGKWFLIILAFQLLIMNGVF
                     IADGAGKLEFEDLTLQLYMGGTLTEVFGLVLVVTKYLFKRK"
BASE COUNT         1809 a         1573 c         1457 g         1649 t
ORIGIN      
        1 ggacgccatc gtatcttttt atcgtgcgaa ctcctcttat ctaaattgtt caactttaag
       61 agaatggcta agcggcgaag cagagccttc agatgagata cttcagaaga ttatggttca
      121 gaacaactgg cgcgcaacaa aatggtcaaa tcagttacct taatctgttg aatgtgtgcc
      181 gccttaaaac aaaaaggcgg cctctcgtta aagtaaatta ccctattttc caccaaggca
      241 tgaagcctct gccccggttg aatcaataca accaccgttc gcagtagtaa tcttcgtacc
      301 cggcgcgatc atcacgtgtt taatactgct gccgtcagcg cgagtcgctt cggtgtatgt
      361 cgaatgtgtg cagcctgcca gcgtgaagaa cgcgacagct aagagaagtt ttttcacgtt
      421 ttacctacgc ttctgagata gatttctcag cattcaccaa tggaagtgat tgtggaacat
      481 ccgtttgacc tgctggccag cggtagcctg ttacgcggga acgaggaaaa gctttgatat
      541 ttacttcatc tgcctggttg ccccccaaaa tcattaaatc cccggcggca tttttcccga
      601 caacaaagcc gacgtgacca ccgccagacc gagacagaat agcaatgcat ccataggcgg
      661 gttccttcag ttcattaccc caatcgagat aagaattcgc agactcaaaa cgcgttgagc
      721 gaatacccgc acgttccagc attgccccgg tgaaagcagc gcaccacggt gtttcgtcat
      781 ccttaatgcc gccgcgtttg atatctttcc agtactgcac aatttctgca gcatgttgct
      841 tccctttcat ttcccgaatg ccgaggtttt ttcgagcctc gattagccat ttaggatccg
      901 tcattacctg tctcctgtta tttttgaaat attccctcgt gcccgccaga cagcaataca
      961 gatggtgagg ttgagaaaga actctgcggg atcaacctgc gtatatttcc cgtaaaaaat
     1021 tcgtagggct atccacgcag cagacagaat cgcgaggtaa gcagttagtg agatgaaaaa
     1081 acgatagcgg cctgtttttt gaaaaaacat caaccgtagt gccaacatca gacaaataat
     1141 tgcattggca ttgatgagga cagagtgcag ggtcattatt tcatcccctt atctgatttc
     1201 cataaacgag cttgtgacat gatccgcaac agtgatgtaa cgctgattac tgatgcaacc
     1261 agtgcaccaa tggcgggaga gacttttact gttacgggag ggttcaggtg actcagcacc
     1321 gcgttcagaa taccggtaat gatggctgac gctgtctcag cgcaatacac accgccaaca
     1381 aacgaaatga gtgcgaaaat aacctgcttc cagaatcgat gctgcccgga cgacaacaca
     1441 tagagagctg cgcctgccag cgcacagatc atcactgcag gtgttgcctc aggaaataac
     1501 gcagcaaacg tcacccccgc cgagccagca gcaacgccag cggttagagg ttcagacatg
     1561 atttttcctg attcagaaac gacaaaaccc cgccgtggcg aggttcttag aaatttggca
     1621 acataccaaa ttagcgtcaa atatggccta ttttgttcgt ttttgcaagc attatacgca
     1681 aacgtcgtct aaatggttct gataatgatg caaagttgca agaagtgcca cttcatcaag
     1741 tgctctcacc gcctctttca ttcctaccca gtgcagggag tatgtttcgc accaggtgga
     1801 acgagaaacc gacagcattc ctgctagggt agatccggcc atctccttaa aatcctcgcg
     1861 tttatttgct gctgcgaccg cctgcacgga caaccacacc aacgaaagca ggcgcttaac
     1921 tacttttctc tgagttgacg cggggatgtt tcctttatag cgactccaga cagcctcaca
     1981 aatagctgtt tggtgtttga atgccaggtt accaccgtag caataacgca accacgcttg
     2041 ctgacttgca tccagcgagt tcacagaacg ccgccaggat gaatcagcaa attcaaaatc
     2101 actcatcggc ggcgcggggc gacgtctgct gaaactttcc agagcgtaaa ctggcgtaac
     2161 cagagcatca acggtccggt cctccagctg cacacggtga atatgtttgc gtgggtatgc
     2221 gtttttgtct gccggtgggt gctcacacaa tgcttccagt tgccccttag aaccaccaga
     2281 caaatcccgc aatgcagcgc gcagttttat gcgggtatat tccaataaat actcgttact
     2341 catcagataa actcctcatc gcgccagata gccagtgttc tgaaaactcc ctcagcgtgc
     2401 atcaacaaca atgagtcgtg ctcgtaatcc gtttttgtac gtccgtcgat ggcatcgtga
     2461 cacgcactgc aggcgatagc gccctgcata tcgtgaggtt tgatggctgt gccgcacgtt
     2521 ccggcgagac gataatgagc cagtacgctg gtttcaggat tgtgattgca cacaccgggg
     2581 atacgcacag tacaagcgcg actgcgtgca gcttttctca ggtcgatttt tttcatgatg
     2641 tgtaatccag cagctgcgcg gcggcatttt cagcggcctc ctgagaggca aatgtgcgga
     2701 acaaaatata attccacagc acatctaaaa cggctgaata cagttgaccg aactcaagat
     2761 cgtccatcgc tgcaaacgag attgaacgag gtacgcgcat caaactgccg tctggcatct
     2821 gatgaacgtc ataaaaccca gcttccataa tcgcccatgc gcgaaacgcc tcgaacgact
     2881 tcaccgcact gatactcccc gcgcgctttt ccgactcatc gagaagatac tgatcagcca
     2941 gctctgccat gacatcaccg tgtccaccgt aatacgccag taacttaacg tagccgtgaa
     3001 ccagttcgcg ctccgcagga gaaatagccc ctccggacgg atgccagtaa tcaaagccca
     3061 ggttgagaag tgagaaaaat ttacgatgaa acgcgggatt acgcgcctgt ttgaaatctg
     3121 catacacaac agcacctggt ttgactcgtt cagtcaacca gcgttgcgtg tctggtgtcg
     3181 cgggtattag cgaaccgccg gataccctga taaatgaatg ctgcgccatg gttaactcca
     3241 ggtggcgcgg cagtgttcag aaaatggtgc cgggtgttca atccagcacc gatattatac
     3301 cgtgttttac gcgtcagggg gtgtaatagt atatccagct atttctgcca actcgagaaa
     3361 cgccatcagg ctggcaatcc gctcatcatc ggtcagaaca cgcacaccaa cgatgtgtcc
     3421 gtccttacgc ttgataacag cccggcatgt ttccggtagt tcgcctatag attcttgaat
     3481 attcatgtct tccccccaat gccacaacct gtatatttat acagtatttt atggcatttg
     3541 tcataacgcc aaatacaatg agggaataat gaacaattat attcacgtcg tgaattttat
     3601 taaacggttt tataggggat taaaccgttt atggtcatta tttatcactc ctgtaaattg
     3661 gaattaacca ttttctaatc gtcgacgctg caggtgggtc agagtatttc gccacaaaaa
     3721 taccggatat gtattgcacg ccacgcgata atggcgctgt cgggttttca gtaaaaaaac
     3781 gatcagccag ttcaatggct tcgcttttta atctgtgctg tggtcgtcct ccgccctttg
     3841 ttcccaggta tcgcgccagt tgtttgaaac ggtaggcgat gattaacggt cgcatcatca
     3901 tattgaaaaa atcgcgggca tcgttgtcag cgccttgcgc tatcaacagt gcaaaaaaca
     3961 gatacgggat tatgtacact tcaggagata tacgaacccg ttcacaagtt ggtggagcca
     4021 actcaataag taagtcagcc agcggattcc ccttttctcc acgtttgtaa tagcgataca
     4081 gcggcccgat atcaagttca ctcacgtcga tttcaggatg cccggcaacg actgtacgta
     4141 tctcttctgc cagagcgtca aatattgccc cggtccgttc aagatagagc ctccgggtcg
     4201 aaccgcgatc atccaacgcc cgtaacatat ctaaccggct aacgaaagtt tcaggccagt
     4261 cctcatagaa ggttttcata attacctcga ataataaaaa ctgcactatt tgcacaattt
     4321 attattcgtt ttcacgccgc tgagttctat aaaaaagaaa aaacctccga ggcagggttt
     4381 tgttatggct cgttaaatca tcatccaact ttttagactg aacggtgata cttgcaaata
     4441 tttgaaaata tgggctggaa ccctatctta tgatgtgcct ggtaatttat attgttgggt
     4501 ctttatatca tgaggaatca ctatgggtac gttaggcaac cagccgtgca gaagtcgtta
     4561 tgagttcgat gattttaatt tagccaatcg agctgcggct cttactaagt tagcaaaaga
     4621 aactggtgta tcggtggata ccttaatcaa acttgaaatt gctatgcaac tgaatcggct
     4681 caactccatc cttatcgaaa acggtgacaa gatggacgag aatctggcag gtattggcga
     4741 gatactgcag aagtgagagt agcccctctt ctgaggggtt gcacatttaa aatattaagg
     4801 gcggtcttct tcacgtacac agtcacctgg tcgaatcgtt cacgcatact gagtacaaaa
     4861 aatctcaacg ataagatcag gttagacctg tattttccag aaacatatag acgcctaaaa
     4921 aaacactaaa ctcatgatta tcatgtatgt ttttactgga aacattgaac catccacaat
     4981 acagcgtaaa aagcaagcaa ttcacaagat tttaaagtga tttatctgga agactttact
     5041 actaacagtt aatatcgtac cagaagtgca gaatttgcac ttttacagga atgtggtacg
     5101 gaacatgctg tctatatttc aaccaataat cgtaatgcag taactggagg agctatggct
     5161 accaacccaa gagagcacgc ccggcgagtg ctggacacgt tctggtgcgg gcgtggtttc
     5221 cctgttgatc cagcgaaaat tgctcacgac atgggattag atgtttttat taccgatctt
     5281 cctgggaaag tttctggcgc actgattaaa caaaaagatc aagatccagc gatctttcta
     5341 aacagtgatg acaacaaagt tcgtcaacgt ttttcatgtg ggcatgagtt ggggcattac
     5401 atcgcccggc aagcatcgca tagcgatgaa tatgagtacg ttgatttacg tggcgaaacc
     5461 gcaagcgccg gaacgcatcc tgatgaaatt tttgcaaaca agttcgcagc agaacttctg
     5521 atgccggtag atgaggtcag aaaactgcac gctgacggtc aaccaagtta tataatggcg
     5581 cattattttg gcgtatctga cgatgcgatg acttatcgtt taaagaactt gaggttgggg
     5641 taacgcttta aaatgccatc ggatgacgat tttaacagcc gtttcaaaca ggaagtagca
     5701 cgcagactga aggattccct caacctacac cgtcagttag cagaagaggt tgagccggag
     5761 gacgatgaaa ctccggttca gtctcctgac gaagcttcac ctcagccaaa ggtttcgcac
     5821 gcccccaaaa aaacggctgt ccgtattcgc ggaatagcca gaaaaagagc tgccgaagct
     5881 cgcaaaatgg ctgtagctgc gtctagcaaa agaattccaa ctgctacacc ctctcaacaa
     5941 gtcacccgta ctgagagtaa aatcttgacc gagttcacat ctgtgaaaca ggtgaattca
     6001 cttgataccg agcatgagcg caaacaactc aacaacaaag attttgaagc agaaatcgct
     6061 ctaaaaaaac gatacgggaa atggttcctg attatccttg ctttccaact tctgataatg
     6121 aatggtgtat ttattgccga cggcgcaggt aagctggaat ttgaggattt aacactacag
     6181 ttgtacatgg gcggtacttt gacagaggta ttcggcctcg tgctggtcgt gacaaaatac
     6241 ctttttaaac gcaagtaact ctctcggtcc aacagtcttt cgaaggccct gatcaaaggg
     6301 ccttgataaa ttagcagtgc gttgaaaaca gatagttatt cccatcagtt ccaacgtaat
     6361 acacatcaaa aaaatcacca ttcttttgct ctaacgtcaa cacctaccct taaaaaaata
     6421 attctccaag aatgagaaag ttattgtgtg tattagataa gatatgagct gacacatcta
     6481 tggcgtgg
//
LOCUS       DAOFSV010000199         6405 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.199, whole genome shotgun sequence.
ACCESSION   DAOFSV010000199 DAOFSV010000000
VERSION     DAOFSV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.199"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(5..631)
                     /gene="upp"
                     /locus_tag="QBH27_004350"
     CDS             complement(5..631)
                     /gene="upp"
                     /locus_tag="QBH27_004350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDQ7016584.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            956..1993
                     /gene="purM"
                     /locus_tag="QBH27_004351"
     CDS             956..1993
                     /gene="purM"
                     /locus_tag="QBH27_004351"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDQ7016585.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1993..2631
                     /gene="purN"
                     /locus_tag="QBH27_004352"
     CDS             1993..2631
                     /gene="purN"
                     /locus_tag="QBH27_004352"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDQ7016586.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2803..4869
                     /gene="ppk1"
                     /locus_tag="QBH27_004353"
     CDS             2803..4869
                     /gene="ppk1"
                     /locus_tag="QBH27_004353"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDQ7016587.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            4874..>6405
                     /gene="ppx"
                     /locus_tag="QBH27_004354"
     CDS             4874..>6405
                     /gene="ppx"
                     /locus_tag="QBH27_004354"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDQ7016588.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PE"
BASE COUNT         1564 a         1589 c         1647 g         1605 t
ORIGIN      
        1 ttctttattt cgtaccaaag attttgtcac cggcatcgcc gaggcccgga ataatgtatc
       61 cgtgctcgtt cagtccctgg tcaatcgatg cagtatacag ttcgacgtcc gggtgcgctt
      121 tttccagcgc agcgatacct tctggtgcag ctaccagcac cagaactttg atgctgctgc
      181 agcccgcttt tttcagcagg tcgatggtcg cgataacgga accaccggtt gccagcattg
      241 ggtcaacgat cagcgccata cgctcatcga tgttagaaac cagtttctgg aagtacggta
      301 ccggctccag cgtttcttca ttacggtaca taccgacgac gctgatgcgc gcgctcggaa
      361 cgttttccag cacaccgtcc atcatcccca gacccgcacg cagaattggc acaacggtaa
      421 ttttcttacc tttgatctgg tcgatttcta ccgggccgtt ccagccttcg atagttactt
      481 tttccgtttc gaggtcggcg gtcgcttcgt aagtcagcag gctacccact tcggaagcga
      541 gttcgcgaaa gcgcttggtg ctgatatctt gctcacgcat cagtcccagc ttgtgtttga
      601 cgagtgggtg tttgacttcc acgatcttca tactctttct cctttgaggg gcagccacaa
      661 aaaaaatcga cggattatac ctcctttctt caaggcggca atattatttt cgttgacttt
      721 agtcaaaatg ataacggttt gagataaagt tattttatat tcaaatagtt atgaaagaag
      781 attattccat ccgaaaacga tcctttgccc tggtacaagt attctttcgc cgcgcgcctg
      841 gggaaaagac gtgcaaaaag gttgtgtaaa gcagtctcgc aaacgtttgc tttccctgtt
      901 agaattgcgc cgaattttat ttttctaccg caagtaacgc gtggggaccc aagcagtgac
      961 cgataaaacc tctcttagct acaaagatgc cggtgttgat attgacgcgg gtaatgctct
     1021 ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct
     1081 gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat cgtgaacctg tgctggtttc
     1141 cggcactgac ggcgtaggta ccaagctgcg tctggcgatg gacttaaaac gtcacgacac
     1201 cattggtatt gatctggtcg ccatgtgcgt taatgacctg gtggtgcaag gtgcggaacc
     1261 gctgtttttc ctcgactatt acgcaaccgg aaaactggat gttgataccg cttcagcggt
     1321 gatcagcggc attgcggaag gttgtctgca atcgggctgt tcactggtgg gtggcgaaac
     1381 ggcggaaatg ccggggatgt atcacggtga agattacgat gtcgctggtt tctgcgttgg
     1441 cgtggtagaa aaatcagaaa tcatcgacgg atctaaagtc agcgacggcg atgtgctgat
     1501 tgcactcggc tccagcggcc cgcactcgaa cggctattcg ctggtgcgca aaattcttga
     1561 agtcagcggt tgtgatccgc aaaccaccga acttgatggt aagccattag ccgatcatct
     1621 gctggcaccg acccgcattt acgtgaagtc agtgctgaaa ttgattgaaa aggtcgatgt
     1681 gcatgccatt gcgcacctga ccggcggcgg cttctgggaa aacattccgc gcgtattgcc
     1741 agataatacc caggcagtga ttgatgaatc ttcctggcag tggccggaag tgttcaactg
     1801 gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt
     1861 cggtatgatt attgccctgc ctgctccgga agtggacaaa gccctcgccc tgctcaatgc
     1921 caacggtgaa aacgcgtgga aaatcggtat catcaaagcc tctgattccg aacaacgcgt
     1981 ggttatcgaa taatgaatat tgtggtgctt atttccggca acggaagtaa tttgcaggca
     2041 attattgacg cctgcaaaac caacaaaatt aaaggcaccg tacgggcagt tttcagcaat
     2101 aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc
     2161 atcaccagcg cgtttgacag tcgtgaagcc tatgaccggg agttgattca tgaaatcgac
     2221 atgtacgcac ccgatgtggt cgtgctggct ggttttatgc gcattctcag cccggcgttt
     2281 gtctcccact atgctgggcg tttgctgaac attcaccctt ctctgctgcc gaaatatccc
     2341 ggattacaca cccatcgtca ggcgctggaa aatggcgatg aagagcacgg tacatcggtg
     2401 catttcgtca ccgatgaact ggacggtggc ccggttattt tacaggcgaa agtcccggta
     2461 tttgctggtg atacggaaga tgacatcacc gcccgcgtgc aaacccagga acacgctatt
     2521 tatccactgg tgattagctg gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg
     2581 tggctggatg gtcaacgtct gccgccgcag ggctacgctg ccgacgagta atgcccccgt
     2641 agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc gcaagctcca
     2701 gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc tcgccataat
     2761 atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca ggaaaagcta
     2821 tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg
     2881 gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc caataacctt
     2941 gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa
     3001 caaggttcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa
     3061 gccgatcagg aattcgacgg cctctacaac gagttgttgc tagagatggc gcgcaaccag
     3121 atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct gcgtcattat
     3181 tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga cactgattta
     3241 gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg tggcgatacc
     3301 atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt gaatttaccg
     3361 ccagaagcgc cgcgtcgacg caagccgatg attcttctgg ataacattct gcgttactgc
     3421 cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta ttcaatgaag
     3481 atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct gatggagttg
     3541 atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc
     3601 gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac
     3661 tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt ccccaatgtc
     3721 ggcaaagcca atctggtgaa caaaccactg ccacgtttac gccacatttg gtttgataaa
     3781 gcccagttcc gcaacggttt tgatgccatt cgtgagcgcg atgtgctgct ctattatcct
     3841 tatcatacct ttgagcatgt actggaactg ctgcgtcagg cttcgttcga cccgagcgta
     3901 ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt cacgcatcat cgactcgatg
     3961 atccacgccg cacacaacgg taagaaagtc accgtggtgg ttgagttaca ggcacgtttc
     4021 gacgaagaag ccaacattca ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc
     4081 ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg taaagaaaac
     4141 ggtgaagtgg tgcgttacgc acacatcggg accgggaact ttaacgaaaa aaccgcgcgt
     4201 ctttatactg actattcgtt gctgaccgcc gatgcgcgca tcactaacga agtacggcgg
     4261 gtgttcaact ttattgaaaa cccataccgt ccggtgacat ttgattattt aatggtgtcg
     4321 ccgcaaaact ctcgccgtct gttgtatgaa atggtggacc gcgagatcgc caacgcgcag
     4381 caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa aggtctggtt
     4441 gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt gcgcggaatg
     4501 tgttctctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt
     4561 gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga taaaaaggtc
     4621 tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga agtggcaacg
     4681 ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat attgttcagc
     4741 gatacggtca aagcacgtta tatcgataaa gaactcagta atcgctacgt tccccgcggc
     4801 aatcgccgca aagtacgggc gcagttggcg atttacgact acatcaaatc actcgaacaa
     4861 tctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt ttgctgcggt
     4921 cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg gtgccatgca
     4981 gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc cagataatat
     5041 gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctatttg ccgaaaggct
     5101 acaagggttt tcccctgcca gcgtctgtat agttggtacc catacgctgc gtcaggcgct
     5161 gaacgccact gactttctga aacgcgcgga aaaagtcatt ccctacccga tcgaaattat
     5221 ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc aaccggaaaa
     5281 aggtcgcaaa ctggttattg atattggcgg cggttctaca gaactggtga ttggtgaaaa
     5341 tttcgaaccg attctcgttg aaagccgccg gatgggttgt gtcagctttg cccagcttta
     5401 tttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgcatgg cggcagcaca
     5461 aaaactggaa acgttaacct ggcaatttcg aattcagggc tggaacgtgg caatgggcgc
     5521 ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga aagacgggat
     5581 aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacgtcacc gtaatttcgc
     5641 atcgctgagt ttaccgggtc tttccgaaga acggaaaaca gtcttcgttc cgggactggc
     5701 gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt ctgacggggc
     5761 gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc cgtcatcagg atgtgcgtag
     5821 tcgcactgcc agcagcctcg ccaaccagta tcacatcgac agcgaacagg cccgacgggt
     5881 gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga agctggcgca
     5941 tccacaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg ggttgaatat
     6001 caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagcg acttgccggg
     6061 ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc gtaaagcgat
     6121 taaactcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc tgccactgat
     6181 tcagctattg cgccttggcg tattactcaa taatcaacgt caggcaacca ccacaccgcc
     6241 aacattgacg ctgattactg atgacagtca ctggacactg cgtttcccgc atgactggtt
     6301 tagtcagaat gcgctggtac tgcttgatct ggaaaaggag caagaatact gggaaggcgt
     6361 ggctggctgg cggttgaaaa ttgaagaaga aagtacacca gaaat
//
LOCUS       DAOFSV010000200         6346 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.200, whole genome shotgun sequence.
ACCESSION   DAOFSV010000200 DAOFSV010000000
VERSION     DAOFSV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.200"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1201
                     /gene="helD"
                     /locus_tag="QBH27_004355"
     CDS             <1..1201
                     /gene="helD"
                     /locus_tag="QBH27_004355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDQ7016589.1"
                     /translation="GSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWL
                     TEEMQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSK
                     RIKLMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQ
                     RAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYR
                     FNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEE
                     RILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPA
                     AARESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLD
                     VPVARKP"
     gene            complement(1233..1691)
                     /gene="mgsA"
                     /locus_tag="QBH27_004356"
     CDS             complement(1233..1691)
                     /gene="mgsA"
                     /locus_tag="QBH27_004356"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDQ7016590.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(1787..2449)
                     /locus_tag="QBH27_004357"
     CDS             complement(1787..2449)
                     /locus_tag="QBH27_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDQ7016591.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            2622..3035
                     /locus_tag="QBH27_004358"
     CDS             2622..3035
                     /locus_tag="QBH27_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDQ7016592.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(3080..3397)
                     /gene="hspQ"
                     /locus_tag="QBH27_004359"
     CDS             complement(3080..3397)
                     /gene="hspQ"
                     /locus_tag="QBH27_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDQ7016593.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(3455..4645)
                     /gene="rlmI"
                     /locus_tag="QBH27_004360"
     CDS             complement(3455..4645)
                     /gene="rlmI"
                     /locus_tag="QBH27_004360"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDQ7016594.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            4740..5018
                     /gene="yccX"
                     /locus_tag="QBH27_004361"
     CDS             4740..5018
                     /gene="yccX"
                     /locus_tag="QBH27_004361"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDQ7016595.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(5015..5344)
                     /gene="tusE"
                     /locus_tag="QBH27_004362"
     CDS             complement(5015..5344)
                     /gene="tusE"
                     /locus_tag="QBH27_004362"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDQ7016596.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(5436..6095)
                     /gene="yccA"
                     /locus_tag="QBH27_004363"
     CDS             complement(5436..6095)
                     /gene="yccA"
                     /locus_tag="QBH27_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDQ7016597.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
BASE COUNT         1659 a         1563 c         1646 g         1478 t
ORIGIN      
        1 gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga
       61 actttttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg
      121 gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaatt tctgggatga
      181 tgaaaaatta cagcgtcgtc ttgcctctcg cctcgatcgc tgggtaagtc tgatgcggat
      241 gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt
      301 cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc
      361 agaaaacgct gtcgattttt cgggccttat tcaccaggcg attgtgattc tggagaaagg
      421 tcgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca
      481 gcgtgcagcg ttgttagcgg cattacgcaa gcaaaatagt caaacgacgt tgttcgctgt
      541 tggtgatgac tggcaggcga tttaccgatt cagcggggcg caaatgtcgc tcaccacggc
      601 tttccatgaa aactttggtg aaggtgatcg ctgtgattta gacacgacct accgttttaa
      661 cagtcgtatc ggtgaggtgg caaacaggtt tattcagcag aacccaggcc agcttaaaaa
      721 gccgctaaac agcttaacca atggagacaa aaaagccgtc acattattgg atgaggggca
      781 acttgacgct ttgctggata agctctctgg ttatgccaaa ccggaagagc gcattctgat
      841 cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc
      901 gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt
      961 catcatcgtt ggcttgcagg aggggagtga tggttttccg gctgcggcgc gggagtcgat
     1021 tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag aacggcggtt
     1081 aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta acaaagagaa
     1141 tccctctcct tttgtcgaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta
     1201 agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc
     1261 tgataatcgg ggatcagaat atcgaccgcg tcgttaaaat gcggtgactg gataatgaag
     1321 tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgttgccag acgcagcaag
     1381 gctttcacgt caggatcgtg cggcacggca tttagtgggt cccagaagaa aatcaataca
     1441 tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc
     1501 aacatcgcgt tgacgttcat gccggtcgcg cgggaaatta agttaccggt agtgcctgtt
     1561 gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct catcagcatt
     1621 tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgaaggtaa agtgcgagtc
     1681 gtcagttcca taatgtacat ccgtagttaa ctttcctaca gattactgta agtacttatc
     1741 gctgcaagat aaagactgaa aaagcctgcg cacaggcaca aaaatctcag gaggatggtt
     1801 gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat
     1861 acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa
     1921 cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc
     1981 gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg
     2041 aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt
     2101 caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc
     2161 gaaaattgac ctggttgatg agctgggtat taaaactaac caccagtgga ggggagatat
     2221 agagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat
     2281 gcggtccatt gtccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct
     2341 taccgtcgag taccagaaga tcgacatcag tagaaagccg cagcgtggtg gcaaaaacgg
     2401 ataccggcaa acataatgca atcaaggtgg tcacgatgcc ggttttcata gatagctcct
     2461 gttaaaagac cgcccgaata ataatgtgtc aaaatttttc tttaatcata cttactaaca
     2521 tatagacata tttcccgctt ttgctctcat tcattcgtat tagctgcatg gttggcatgt
     2581 cggctttgtc gtacactttg ttaaacagtc tggaggaaat aatgaaagaa accgatattg
     2641 caggcatttt aacgtccact cacaccattg cgctggtggg ggcaagcgac aaacccgatc
     2701 gtcccagcta tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc attcctgttt
     2761 cgccaaaagt tgccggcaaa acactgctgg gccagcaggg gtatggcacg ctggcagacg
     2821 ttccggaaaa agtcgatatg gtcgatgttt tccgtaattc agaagcggcg tggggcgtgg
     2881 cgcaggaagc tatcgccatt ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg
     2941 agcaagcggc agtactggca cgggatgccg ggttaaatgt ggtgatggat cgctgcccgg
     3001 ctattgagat ccctcgcctg ggcctggcaa aataaaaaat acccggaagg cgaaaacctt
     3061 ccgggtatgt ttatgagaat tagttacgca gacgcggcgc ctggagttgt ttgcggatgg
     3121 tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta
     3181 gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcatcctcc atcaccacgt
     3241 gataccacgg agcggcgcga agctcgtcat tcaccgccag ctcatcaggc gacggttcag
     3301 agagcgagta aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc
     3361 ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta
     3421 cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt
     3481 tcagatatag cccttccggg taggtagcga tcaccggatg atcggcggcc tgacggaact
     3541 gctctataaa ttgtacatca cgaccggcat caattgcggc atccgcgatg attttctgaa
     3601 ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaacaccg ccttcattca
     3661 gcaactgaat cgccagcatg ttgatgtctt tatagccacg gcacgcgccc atcaactggc
     3721 ttttattctc aacaaacttc ggcgggtcca tcacgataac atcaaatttt tcaccgcgat
     3781 cgcgataagt acgcagcaat ttaaagacat catcacggac aaactctgcc ttgctcagat
     3841 ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctgg gaggtatcaa
     3901 cgctgacaac ctggctgcaa ccgcccatca atgccgatac ggcgaaacca ccggtatagg
     3961 agaaacagtt aagtacgcgt ttattttcga cgtagcggcg ggtagccagg cggctgtcac
     4021 gctggtccag gtagtagccc gttttgtgcc cgtgctgaat atccaccagc agtttcatgc
     4081 cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc ggtgatgggg ccctgagtca
     4141 gctccatccc ttctttttta cgtaccgcga cgtcgctgcg atcgtaaatg gcacattccg
     4201 ggtacaacgt ttgcagggcg ctaattaatg ccgcgcgctg atactctgcg ccagcactca
     4261 gcaattgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag
     4321 attcaccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc
     4381 atttttgtgc ttgctgcaaa cggcgggtaa aaaaagcaat gtcgatagac tcagacgggt
     4441 caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg cgtgctaacc
     4501 attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatgc
     4561 gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt
     4621 tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt
     4681 cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa
     4741 tgtcgaaagt ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggattccgct
     4801 acaccacgca gtacgaagcg aaaaaactgg gactaaccgg gtacgccaaa aatcttgacg
     4861 atggcagcgt ggaagtggtt gcctgcggtg aagaagggca ggtggaaaaa ttaatgcagt
     4921 ggttaaaaag tggcggcccg cgttctgcgc gtgtggaacg ggtgctcagc gagccgcatc
     4981 atccctccgg ggagttaacg gattttcgta tccgttaaat gcattttacc ggtttaggca
     5041 ggccagcaat tttggtggct tgctttgccg gacctttcgg gaacagtcgg tacagatagc
     5101 ggctattgcc tttctcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg
     5161 ccggagaagt attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt
     5221 ctggcgacag agtaatccct tcgttttctg caatcaccac cgccagcggc tcactccact
     5281 ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca
     5341 gcataattct ttactacctt aatcagactg gcggcagtgt aacaaaaaac aaagccccgc
     5401 ataaagcgag gctcggtaag taaccctggg cgagattaat cgcggctggc gaagcccaga
     5461 atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac cgtggcacga
     5521 atatagttcg tttcaccgcc atgaatgatg ttgctggttt caaacaagat agcgccagag
     5581 gagatcagaa tgaagaccgc gctgatcgcc agatgcagag caggcagttg caggaagata
     5641 ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc gccgaggaaa
     5701 gacatatctt tgcgggtggt cagcacatac gcagagcagc agaagaacac taacgccgtt
     5761 ccgcccagtg ccatagcgat tacgtcaccc attccggcag acagataggt gttcagaatt
     5821 ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat cccggtcggc
     5881 ttattcgccg ttttgtaggt caggaacatc aaaccataca tacccaccag cgtcagaatc
     5941 agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc
     6001 agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct aagcagcgat
     6061 gtacggtcat gtgaagaact aactatacga tccattgagt cactctctat gacagatgta
     6121 attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac ccatctttac
     6181 gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaggcag tgatataacg
     6241 ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac cccttatctt
     6301 atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtc
//
LOCUS       DAOFSV010000201         6323 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.201, whole genome shotgun sequence.
ACCESSION   DAOFSV010000201 DAOFSV010000000
VERSION     DAOFSV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.201"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..124)
                     /locus_tag="QBH27_004364"
     CDS             complement(<1..124)
                     /locus_tag="QBH27_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016598.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNA"
     gene            complement(224..580)
                     /locus_tag="QBH27_004365"
     CDS             complement(224..580)
                     /locus_tag="QBH27_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDQ7016599.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DYVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(716..823)
                     /gene="rprA"
                     /locus_tag="QBH27_004366"
     ncRNA           complement(716..823)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="QBH27_004366"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(1009..2121)
                     /gene="ydiK"
                     /locus_tag="QBH27_004367"
     CDS             complement(1009..2121)
                     /gene="ydiK"
                     /locus_tag="QBH27_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDQ7016600.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            2335..5391
                     /locus_tag="QBH27_004368"
     CDS             2335..5391
                     /locus_tag="QBH27_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDQ7016601.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            5388..5798
                     /gene="menI"
                     /locus_tag="QBH27_004369"
     CDS             5388..5798
                     /gene="menI"
                     /locus_tag="QBH27_004369"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDQ7016602.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSVREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5898..6086
                     /locus_tag="QBH27_004370"
     CDS             5898..6086
                     /locus_tag="QBH27_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDQ7016603.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
BASE COUNT         1622 a         1545 c         1652 g         1504 t
ORIGIN      
        1 cggcattagt ctgccaaagc gtctccagcg aggccatatt caggctcatc aaaatgacgc
       61 ccataccatg caccaggtaa ttaaaataca acccaagtgc ggtagggtaa tagggatttt
      121 tcataggttc tacttagcaa aggtgcttta aaaagtgtag cgatgttgtg tcagtatccg
      181 ttcattattg ttgtaacaac gacaattcct ttctgtggag aaattaacaa agccgctcca
      241 gatcgtgtgc atataattca gcggctaccg attgatagat cttccagtca ataaaattgc
      301 catcagggta gacttgttga aacgcggtta agtcggggaa aaaacgcatg gtgttgttgc
      361 cgatgcggtt atttattttc tctatgattg catgaagatg tttttttcgt tgctgacgca
      421 tagccaacag ttgctcgact acataatcag gaatggcgca tttgccattt tcccactgac
      481 gccagctttc actgttaccg gtctgggcaa tccaggtcgc gcattcatca atagtcatgg
      541 caaaaatatg gcgaagtgcc tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa
      601 taaacaataa aatataaata acattatcag cctgctgacg gcttgaagag agtcacagta
      661 tcttgtgcaa cattattgga aagagtgagg ggcgaggtag cgaagcagaa aaatgttaaa
      721 aaaaagccca tcgtgggaga tgggcaaaga ctacacacag caattcgttg tttcactcag
      781 gggatttcca tgcttataaa tcaatatgtt gatttataac cgtgagctaa tagtaggcat
      841 acggactttt tgcgtcgatc agattcgtct cattagttta aaaactgtaa agaatttgcg
      901 acaggtacta ccagctgaat agcggtaagc aggaaaatat ggggaagagc aagcaaatat
      961 caattagcag gggttaacaa tcaggcggca gagccgcctg atgagaaatt acttattcga
     1021 tttctcgatt tcgccaagtt cttcgagaat ttcttccggt tggtccgtcg gcggtgggac
     1081 ttcttccacc cacgcggcaa acagacgcca ggaaacggct aacagaaccg gaccaataaa
     1141 cagaccgatc atcccgaaag caatcaaacc accaataacg ccagagagga tcaggatcag
     1201 cggtaaatcg gcacccatgc gaattaacat tgggcggatg acgttatcga gcgtgccaac
     1261 cacaccgctc caaactaaca ataccgttcc ccaggtggta tcgccagtcc agtagagcca
     1321 gataatcgcc ggaatcagta ccggcaacgg tccaagctgg acaaggcagg agaggatcat
     1381 taacaccgtt agcaaagttg cataaggtac gccggatacg gcgaggccga taccgccaag
     1441 cactgcctgt actaacgccg tcaccaccac acccagcgcc accgcgcgga tagcctgtgc
     1501 cgccagcagc acggcggcat ctccgcgaac gcctgccaga cgggttgcaa aatggcgaat
     1561 gccttgtgcc acctgttcac cgcgccagta cagcagggcg ctgaagagaa gcatcagcgc
     1621 acaatgcacc ataaagcgcc cgatatgcgc cgcctgccca acgaaccagg tggtggtggt
     1681 gccaatataa ggacggactt tcgccatgat cgccgtcccc cccatatcca gcaaattgtg
     1741 ccagcctgca tatagcttcg cgccaatcac cggaatggta ttaagccacg ccagatcggg
     1801 taacgtcatg tcaccactgg aaatggcttt aattagcggg ccgctgccgt cgacgatact
     1861 gttaaccagc aaagcaatag ggatgataaa caccatcact aataacagcg tcatcaccag
     1921 aacggcgaga gagcggcgac caaacatgat cttttgcaaa cgtaacaata ccggccaggt
     1981 ggcgataacc actgtaccgg cccatgcaaa gccgagaata aagggttgta caatccacag
     2041 acatgccaca atcatgatgg ctaaaaacag caccgaaagc agaatttgtg cgacatccct
     2101 gggctgacga acatttacca taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg
     2161 cgacgtgaac acgcgaaact cacctgcata atgatgagca atttcagcgg tttttaacag
     2221 gtcgattctg cccttaattc tgatgggcgt gttagaaaaa aatgtggtac aacaactcag
     2281 gcaacacgca aacgattaat ctctcatcac agaccgcagg aaagggtcaa tataatgatt
     2341 ccacagattt cccaggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     2401 gagcaacagg gttttaccgg cgatacggcg acaagttatg ccgatcgtct gacaatgtcg
     2461 actgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     2521 gatgtagcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgtt gatctttacc
     2581 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     2641 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     2701 gaggccgggg tgattaaaga ccaactcaat cagtacctga aaccctttgg ctactttttt
     2761 gcgccagaac tttcgaccag caaccgggca acgctcggcg ggatgatcaa taccgatgca
     2821 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     2881 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgaact ggcggaaacg
     2941 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     3001 cagcaacgcc agttaatcat cgacaacttc cccaagctta accgctttct taccggttac
     3061 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     3121 ggttcagaag ggacgctggc ctttattacc gaagcgcgac tggatattac gcgcttgcct
     3181 aaagtacgcc gtctggtgaa cgtcaaatat gactcttttg actccgcgct gcgtaacgcg
     3241 ccgtttatgg ttgaggcgcg cgcgctttcg gtagagacgg tggactcaaa agtgctcaac
     3301 cttgcacggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tgtgcctgat
     3361 aaagagatgc ttgggctgaa cattgtggaa tttgccggtg atgatgaggc actgattgac
     3421 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcagga
     3481 gtgatcggct ggcaggtgtg ccgcgagctg gcgggtgttg aacgtatcta tgcgatgcgc
     3541 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgctg ctaagccaat tccgtttgct
     3601 gaggatacct gcgtaccgcc ggaacacctg gcggattata ttgctgaatt tcgcgcgctg
     3661 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     3721 gtccgtccgg cgctggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     3781 gatgatgtag tggcgcttac cgcgaaatac ggtggtttgt tgtggggcga acacggcaaa
     3841 ggttttcgcg ccgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgt
     3901 aaagtgaaag cggcatttga cccgcataac cgactcaacc ccgggaagat ttgcccgcca
     3961 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcga
     4021 cagatcccca ttgcggtacg ccagcagtgg cgtggtgcaa tggagtgtaa cggcaacggt
     4081 ttatgcttca actttgatgc tcgtagtccg atgtgtccgt cgatgaagat cacccagaac
     4141 cggattcatt caccgaaagg gcgcgctacg ctggtacgtg aatggctgcg tttgttggcg
     4201 gatcgcggcg ttgacccact caaactggaa caagaacttc ctgaatccgg agtcagtttg
     4261 cggacgttaa ttgcccgcac gcgcaatagt tggcatgcga ataaaggcga atatgacttc
     4321 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcgtg ttcgactcag
     4381 tgccccatca aaattgatgt gccggagttt cgctctcgtt tcctgcaact ctatcacacc
     4441 cgttatttac gcccgctgcg cgaccacctc gtcgctacgg ttgagagtta cgcaccgctg
     4501 atggcacgcg cgccgaaaac ctttaatttc ttcattaacc agccgctggt gcgcaaactc
     4561 tcggaaaaac atatcggcat ggttgatttg ccgctgctgt cggtcccctc gctacaacaa
     4621 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     4681 gagcagaaag cgcgcacggt gcttgtggtg caggacccct ttaccagcta ttacgatgct
     4741 caagtggtgg cggattttgt ccgtctggtc gaaaaattag gtttccagcc cgtgttactg
     4801 ccattttcgc caaatggcaa agcccagcat atcaaaggtt ttcttaatcg ttttgcgaag
     4861 acggcgaaaa agacggcgga tttcctcaac cgtatggcga agctgggtat gccaatggta
     4921 ggcgtcgacc cggcgctggt actttgttat cgcgatgaat ataaactggc tctgggcgag
     4981 gagcgtggcg agtttaacgt cttactggcg aatgaatggc tggcaagcgc acttgagtca
     5041 cagccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaagtt
     5101 accgccttgc cgggcgcgcc agcacaatgg gcggcaatat ttgcccgttt tggcgcgaaa
     5161 ctggaaaatg tcagcgtggg gtgctgcggc atggcaggga cttacggaca tgaagcgaaa
     5221 aatcataaaa attcgctcgg gatctatgag ttatcctggc atcaggcgat gcagcgactg
     5281 ccgcgtaatc gctgtctggc gaccggatat tcctgccgta gtcaggtaaa acgggttgaa
     5341 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     5401 gaaaaatcac cctggaagca ctgaatgcta tgggggaagg aaacatggtg gggttgctgg
     5461 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     5521 ggacaaagca gcctttcggg ttgctgcatg gaggtgcatc cgtggtactg gccgaaagta
     5581 tcggttccgt tgccggttat ttatgtaccg agggtgagca aaaagtggtt ggtctggaaa
     5641 tcaatgctaa ccacgtccgc tcagtacgag aagggcgggt gcgcggcgta tgtaaaccgt
     5701 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
     5761 tgtgctgttc gtcacgattg acgaccgcta ttttgtgacg ttgtcaccag aaaagtgtga
     5821 cgcatattgc ttttggttat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
     5881 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccaactggcc attgaatttt
     5941 tacgccgtga ccaaagcaat ctttcacctg cccaatatct caagcgtctg aaacaattag
     6001 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctatt
     6061 tcgcctggcg tctgggcgtg cattgatcct gatttaatgt cgcgagttcg ccaaatcggc
     6121 ctggattaat cttcgtccag gttcgagggc cttcttaata ctgcctatct acccgcctga
     6181 taacgttcaa aaataataca aatcgcagtt tgtgttaaaa cagtgggtta gctttatgag
     6241 attatcctta tcgccccttc aagagctaag ccactgtgag tgccggagat aagcgccgga
     6301 tggggtagaa acccttaagc ctg
//
LOCUS       DAOFSV010000202         6291 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.202, whole genome shotgun sequence.
ACCESSION   DAOFSV010000202 DAOFSV010000000
VERSION     DAOFSV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.202"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1517
                     /locus_tag="QBH27_004371"
     CDS             <1..1517
                     /locus_tag="QBH27_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDQ7016604.1"
                     /translation="NKTPILKLNNSDDVVIARTELPAGSWLESESMETRDLIPAGHKI
                     ALRDIPAGSAVKRYGQIIGFATRDIRQGEHIHVHNLGMGSFERDYAWGIDSHLPEKDV
                     HQDTFMGIRRKDGRVATRNYIGVLSSVNCSATVVRAIEDHFRTRGLSDYPNVDGIVAL
                     PQSFGCAFGSDSEMMKVMRRTMAGYATHANFAGIVIVGLGCESFQIKDMMNAHGLHES
                     PVFHTMTIQESGGTRNAIEKGISFILDMLPEANNVKREPVPVSEIILALECGGSDSYS
                     GISANPALGYAVDRLVRQGGTAILSETSEIYGAEHLLTRRAISREVGEKLIRRIKWWE
                     EYCKRTGSEMNNNPSAGNKAGGLTTVLEKSLGGIAKAGTTSLMDVYEYAEPVTAKGLV
                     FMDTPGYDPMAVTGQIAGGANLLCFTTGRGSAFGAKPTPCLKLSTNNALWQRQRDDMD
                     LNCGTIVDGEETIEQVGERIYQRIIELASGHRSRSEELGYGSQEFVPWIMNSIM"
     gene            1595..2608
                     /locus_tag="QBH27_004372"
     CDS             1595..2608
                     /locus_tag="QBH27_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDQ7016605.1"
                     /translation="MERKPVLGIFLGEAAGIGPELVAKVVADGTVYKYCRPVLIGDAR
                     VLALGQKIAGVHFQWNVISDPTEADWTTGNVPLLDLKNFNPEKLTMGTIDTDSGYATG
                     ESLVTCMSLLKSGKIDGFVFAPLNKEAFKKGGWDIEDEHYLFAEQLGYLDKPRGLLNV
                     LGDLWVFRVTGHIPFKDIASHITPENVSRSIQLCYDTLRMAAVENPRIAVAALNPHAG
                     DGGTCGKEEIDVLIPVIEGFREKGMDMYGPVPGDTVFIHAFNHEYDAVVTLFHDQGQI
                     ATKLHSFDVGITVAAGLPFAITTPEHGTAFDIAGKGIAKTLATERAIAVAARMASTVH
                     KDN"
     gene            2869..4113
                     /locus_tag="QBH27_004373"
     CDS             2869..4113
                     /locus_tag="QBH27_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016606.1"
                     /translation="MLQIKARTKILALFCAMSFLMYVDRVNLSATAGLIKDELGLSNT
                     ELGVIFSAFAYTYAVFQIIGGWFVDRLGSRRMIIFCGIIWVIATMATGFVGGFISLFL
                     ARLLLGVGEGAALPSQARAITYWFKPQQRGFVQGVTHSFSRLGNAITPPIIVALVAFW
                     SWRGAFISLAIVTFIWLISWIISYKDDPRDHSGMTEEELEGVPIFEKKDLKVKAEPTP
                     WRALIKRMGPTMGVYFCYGWTSWLFFTWLPTFFMHGRGMDLKSSALFTAGVFLSGVVG
                     NTVGGVISDKVLKMTGNLVAARRNVILFSFISVLFLLVPVINTDSLLIMTICLSVAFF
                     CLELTIGPIWAVPMDITPKYVGIASGLMNAGSAVAGIISPIVFGIIIDKTGNWSLPFY
                     GSVALLIIGIFLTFFMRPDKSL"
     gene            4201..4451
                     /locus_tag="QBH27_004374"
                     /pseudo
     CDS             4201..4451
                     /locus_tag="QBH27_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4730..5605
                     /locus_tag="QBH27_004375"
                     /pseudo
     CDS             4730..5605
                     /locus_tag="QBH27_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(5805..6041)
                     /locus_tag="QBH27_004376"
     CDS             complement(5805..6041)
                     /locus_tag="QBH27_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDQ7016607.1"
                     /translation="MVKLALQPRDSVACIAREHDINDNLLFKWLRLWLIKGRISRRLP
                     VTNSSGIGVELLPVEMTLDERPYPVFTRGNYEDQ"
BASE COUNT         1642 a         1304 c         1542 g         1803 t
ORIGIN      
        1 tgaataagac tccgatactc aaattaaata actcggatga tgtcgttatt gccaggacag
       61 aacttcctgc cggaagctgg ctggaatctg aaagtatgga aacacgtgac cttattccgg
      121 cagggcacaa aattgctctt cgggatattc cggccggcag tgccgttaaa cgctatggac
      181 agataatcgg ttttgcgacc cgtgacatcc gacagggcga acatattcat gtgcataatc
      241 tcggtatggg gagttttgag cgtgattatg cctgggggat agacagtcat cttccggaaa
      301 aggatgttca tcaggatacc tttatgggga tccgcagaaa agacggacgg gtggcaaccc
      361 gtaattatat tggtgtactg agttctgtaa actgtagtgc aaccgtagta agggcaattg
      421 aagatcattt ccgtacccgt ggtctcagtg attaccctaa tgtggatggt attgttgccc
      481 tcccacaaag ttttggttgt gcctttggct ctgacagcga aatgatgaaa gtcatgcgga
      541 ggacaatggc aggctatgcc acgcacgcga actttgccgg gatcgtgatc gtgggactgg
      601 gatgtgaatc attccagatt aaggacatga tgaatgccca cggattacat gaaagtcctg
      661 tattccatac aatgactatt caggaaagcg gcggaacacg taacgctata gaaaaaggta
      721 tttccttcat tctcgacatg ctcccggaag ccaacaatgt caaacgagaa ccagttccgg
      781 tatcagaaat cattctggct cttgagtgtg gcggctctga tagctattca ggaatttccg
      841 ctaatccggc tctcgggtac gcagttgatc gcctggttcg ccagggagga acggctattc
      901 tgtcggaaac atcggaaatt tatggagcag aacaccttct gacacgccgt gccattagtc
      961 gcgaagttgg cgagaaacta atccgcagaa ttaagtggtg ggaagagtac tgtaaacgta
     1021 cagggagtga aatgaataac aacccttctg caggtaataa agcgggggga ctgaccactg
     1081 ttctggagaa atcgctggga gggatagcaa aagctggtac aaccagctta atggatgtgt
     1141 atgaatatgc tgaaccggtg acagcaaaag ggcttgtctt tatggatacc cccggatatg
     1201 acccgatggc agtaaccgga caaatcgccg gaggagccaa cctgctgtgt tttaccacgg
     1261 gcaggggatc tgcctttggc gcaaaaccca ctccttgtct gaaactgtca accaataacg
     1321 cactatggca acggcagcgc gacgacatgg atttaaactg tggcacgatt gttgatggcg
     1381 aagaaacaat agaacaggtt ggagaacgta tttatcagag aattattgag ctggcttcag
     1441 gtcatcgtag tcgaagtgaa gagctgggtt atgggagcca ggaattcgtt ccttggataa
     1501 tgaactcaat catgtaataa ctacgggcat aatttgtttt gcccggaaat aaggaaacaa
     1561 actgaatcct gtttttgata attagaggtg aataatggaa cgtaaaccag tactgggcat
     1621 ttttttaggt gaagctgccg gcattggacc tgaactggtt gccaaagtcg tagctgatgg
     1681 tactgtctat aaatattgca gacctgttct gattggcgat gcccgggtac ttgctcttgg
     1741 acaaaagatt gctggtgtgc attttcagtg gaatgtcatc agtgatccca ctgaagctga
     1801 ctggactacg ggaaatgtac cattacttga tttgaaaaat tttaatcccg aaaaactaac
     1861 tatgggcacc attgataccg actcaggtta tgcaacagga gaatcactgg taacctgtat
     1921 gtcattactt aaatcaggta aaattgacgg ttttgtcttc gctccactca ataaagaagc
     1981 tttcaagaag gggggctggg atattgagga cgaacattat ctctttgctg agcagttagg
     2041 atatttggat aaacctcgtg gcctgttgaa tgtccttggt gacctctggg tttttcgcgt
     2101 gacgggacat attccattca aagatattgc cagccatatt acaccagaaa atgtcagtcg
     2161 ttcgattcag ttatgctatg acacattacg tatggcggcc gttgaaaatc cacgaatagc
     2221 ggttgcagca cttaatcctc atgccggtga tggaggaacc tgtggtaagg aagaaattga
     2281 tgttctcatt ccggtcattg aaggcttccg cgagaaaggg atggatatgt atggcccagt
     2341 accgggtgat actgttttta ttcatgcatt taatcatgaa tatgatgctg tcgttaccct
     2401 gttccatgac cagggacaga ttgccaccaa gcttcattcc tttgatgtgg gcataactgt
     2461 agccgcaggg cttccatttg ctattacaac accggaacat ggcactgcat ttgatattgc
     2521 aggcaaggga atagcaaaaa ctctggccac agagcgggct attgcagttg cagctagaat
     2581 ggcatctaca gtgcataaag ataattgaga tctctaaatc ttagtgtatt tatatattct
     2641 tttttgattt tattgaatga agttattttt atgttattcc gccttagcca taagccatgg
     2701 ttaaagcgga ataacaagcc aaaaagcgtc cctcgaagca cgttgaaccg tattatttat
     2761 aattatggtt tacagagtaa aaaaatagat gaatgttggg gcgttgctaa tatatgtaac
     2821 taattgacat tatttactag caatttaacg ctagtttagg agacaaaaat gttacaaata
     2881 aaagccagaa ccaagatact tgcgctattt tgcgcaatgt cattcctaat gtatgtggac
     2941 cgagtaaact tatcagctac tgcggggttg attaaagatg aactcggact gagtaataca
     3001 gaactcggcg ttattttttc tgcttttgct tatacctatg cagtttttca aatcatcggt
     3061 ggctggttcg ttgatcgact tggctcacgc cgcatgatca ttttttgtgg cattatttgg
     3121 gttatagcaa ccatggcgac tggtttcgtc ggtggcttta tatcactatt cctcgccaga
     3181 ttgctattag gtgtagggga aggtgcggcg ctgccttctc aggcgcgagc gatcacctac
     3241 tggttcaagc cgcagcagcg tggctttgtt cagggcgtta cgcactcatt ctctcgtttg
     3301 ggaaatgcga ttactccccc cattattgtt gcattagtgg cattttggtc ttggcgtggc
     3361 gcatttatca gtttggctat tgtcacattt atctggttaa tatcttggat catatcgtat
     3421 aaagatgacc cacgagacca cagcggtatg accgaagaag aactagaagg cgtaccaatt
     3481 ttcgagaaaa aagatctaaa agttaaagct gagcctactc catggagagc cctgataaaa
     3541 cgaatgggac cgacaatggg agtttatttc tgttacggat ggacaagttg gttgtttttt
     3601 acttggttac caactttctt tatgcatggt cgcggcatgg atcttaagtc atcggcgctc
     3661 tttactgccg gcgtatttct gtccggtgta gtaggtaata cggttggtgg cgtaatcagc
     3721 gacaaagtgc tgaagatgac tggcaatcta gtagcagccc ggcgtaacgt cattcttttc
     3781 agctttatct ccgtactatt tctgctcgtc ccggttataa atactgactc gttgctcatt
     3841 atgactatat gtctgagtgt ggcgttcttc tgtcttgagt tgaccatagg gcctatctgg
     3901 gccgtcccga tggatattac gccgaagtat gtgggaattg ccagcggatt gatgaacgcc
     3961 ggttccgctg tcgccggcat tatttcacct atcgtctttg gcatcattat cgacaaaaca
     4021 ggcaactgga gtttaccatt ctacggttcc gtcgcattgc ttattattgg tatatttctg
     4081 acgttcttca tgcgtccaga taagtcattg taaaagtcga attcagggct gaagtggtct
     4141 actgaatttg gccatctgaa cagggtgata tgctcacctt agaacaacac aggtgcacct
     4201 atgaaaagaa gaaatttcag tcctgaattc aaacgtgaat ccgctcagtt ggttgtcgat
     4261 caaaattaca ctgtttctga cgctgctaag gctatggatg ttggtctttc cacgatgacg
     4321 gaatggttca aacaactgcg tgatgagcgt cagggtaaac gccaaaagcc tccctgataa
     4381 caccggaaca aatcaaacta cgtgagctaa agaaaaagct acaacgaatt gaaatggaaa
     4441 acgaaatatt aaaaaacttt ccatgtgtga aaccccgtcc cgagtctcag aaactccccg
     4501 gtaaaactgg cagtctaata tagcgaagga agcagccgtc gaccttttgg tggtatgacc
     4561 cttaaaaaac atggttcagg gagtgtggcg gatccgacag tggtagcttg atcccgttca
     4621 ggaaggtgat ttctccgtcg tctcctcctt cgttcattaa aaatgagcaa ggagatttat
     4681 atgaaaaaat cctcccgaat gtctattgtc cattctcatg ctgcgggcgt tgtttatgtc
     4741 gatgatggta gcggtaattc tggcaacagc aggcatgacc ggcattacgc ttatagccgt
     4801 tggctctttg gttgttggcg tggcaatggt ctttttcccg gctattgctc atccgtacat
     4861 gaagaaagtg accggttctg atgacgttgc catcggccac ttctcaacat tatcttatgt
     4921 attggccggt ttcatcggca gcaagtttgg taataaagag cactcgactg aagacatgaa
     4981 tgtgccgaaa agtctgctgt tcttgcgcga cacgccggtt gctatctctt ttaccatgat
     5041 cataattttc ctgctgacgt gcctgtttgc gggtgcagat gcggtgaaag agcttagcgg
     5101 tggtaaaaac tggttcatgt tctctatcat gcaatccatt accttcgcag ctggcgtgta
     5161 catcattctg cagggtgtgc gcatggtgat tgcggagatt gtcccggcat tcaaaggtat
     5221 ttcagacaag ctggttccga atgccagacc tgctctcgat tgcccggtca tcttccctta
     5281 cgcacctaac gctgtactgg ttggtttcct gagtagcttt gctgcaggtt taatcggtat
     5341 gttcacgttg tatctgctga acatgatcgt gattattcct ggtgtggtgc cgcacttctt
     5401 cgtaggtgca gctgcagggg tattcggtaa tgcaacgggt ggacgtcgtg gtgcgatttt
     5461 gggtgccttt gcgcaaggct tgcttattac cttcctgtca gtattcttat tgcctgtgct
     5521 gggtgatatt ggttttgcta acaccacctt cagtgatgct gactttggcg cactgggtat
     5581 tttgttaggg attatcgttc gctaatattt tgatgacatt aatatcctcc aggatttcag
     5641 gaacgtggag gatattaatt cctcttattt tctccatttt tctttcttcc agttaggact
     5701 gcatctgatt aatcgtaagc gctccgagaa gtacgtagcg taaggattat tttacagctg
     5761 agaagtttca gtacagcagt tcatgcacac ggttcgacgg ctagtcattg atcttcataa
     5821 ttacctcgcg taaaaacggg gtaaggccgc tcatccagcg tcatctccac aggtaataat
     5881 tcaacgccaa tgccagaaga gttcgttacc ggaagacgcc gtgatatacg ccctttgatc
     5941 agccagagcc tgagccattt gaacagaaga ttatcattga tatcgtgctc ccgggcaata
     6001 caggcaacag aatcccttgg ttgtaaagcc agtttaacca tttgaagttt aaactcattt
     6061 gaaaatgacc tgcggggttc tgctgataat gttttttgtt ctataacagg tgtccacttg
     6121 ttgaaaaggt gggtacctat gttaccaata caggcttaat ggctacatac agcagtcggt
     6181 ttacgcatac acttttatag tgcactccgg cctcatgaat ataacggtgg attaccgccg
     6241 aacgaatcgg aaaatagata ctggaaaaaa tctgaaattg tgaccagttt t
//
LOCUS       DAOFSV010000203         6208 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.203, whole genome shotgun sequence.
ACCESSION   DAOFSV010000203 DAOFSV010000000
VERSION     DAOFSV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.203"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            420..1541
                     /gene="ompC"
                     /locus_tag="QBH27_004377"
     CDS             420..1541
                     /gene="ompC"
                     /locus_tag="QBH27_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDQ7016608.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWA
                     NKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1653..2708
                     /gene="apbE"
                     /locus_tag="QBH27_004378"
     CDS             1653..2708
                     /gene="apbE"
                     /locus_tag="QBH27_004378"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDQ7016609.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KAWMSPQFKSFLISEKN"
     gene            2782..3846
                     /gene="ada"
                     /locus_tag="QBH27_004379"
     CDS             2782..3846
                     /gene="ada"
                     /locus_tag="QBH27_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDQ7016610.1"
                     /translation="MKYAIRLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3846..4496
                     /gene="alkB"
                     /locus_tag="QBH27_004380"
     CDS             3846..4496
                     /gene="alkB"
                     /locus_tag="QBH27_004380"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDQ7016611.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4572..>6208
                     /gene="yojI"
                     /locus_tag="QBH27_004381"
     CDS             4572..>6208
                     /gene="yojI"
                     /locus_tag="QBH27_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDQ7016612.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVART"
BASE COUNT         1495 a         1524 c         1674 g         1515 t
ORIGIN      
        1 ccggatgcga ggcatccggt tgaaataggg gtaaacagac attcagaaat gaatgacggt
       61 aataaataaa gttaatgatg atagcgggag atattctagt tgcgagtgaa ggttttgttt
      121 tgacattcag tgctgtcaaa tacttaagaa taagttattg attttaacct tgaattaata
      181 ttgcttgatg ttaggtgctt attttgccat tccgcaataa tcttaaaaag ttcccttgta
      241 tttacatttt gaaacatcta tagcgataaa tgaaacatct taaaagtttt agtatcatat
      301 tcgtgttgga ttattctgca tttttgggga gaatggactt gccgactgat taatgagggt
      361 taatcagtat gcagtggcat aaaaaagcaa ataaaggcat ataacagagg gttaataaca
      421 tgaaagttaa agtactgtcc ctcctggtcc cagctctgct ggtagcaggc gcagcaaacg
      481 ctgctgaagt ttacaacaaa gacggcaaca aattagatct gtacggtaaa gtagacggcc
      541 tgcactattt ctctgacaac aagtcagaag acggcgacca gacctacatg cgtcttggct
      601 tcaaaggtga aactcaggtt actgaccagc tgaccggtta cggccagtgg gaatatcaga
      661 tccagggcaa cagcgctgaa aacgaaaaca actcctggac ccgtgtggca ttcgcaggtc
      721 tgaaattcca ggatgtgggt tctttcgact acggtcgtaa ctacggcgtt gtttatgacg
      781 taacttcctg gaccgacgta ctgccagaat tcggtggcga cacttacggt tctgacaact
      841 tcatgcagca gcgtggtaac ggcttcgcga cctatcgtaa caccgacttc ttcggtctgg
      901 ttgacggcct gaactttgct gttcagtacc agggtaaaaa cggtagcgta gatggcgaag
      961 gcatgaccaa caacggtcgt ggtgccctgc gtcagaacgg cgacggcgtt ggcggatcta
     1021 tcacttatga ttacgaaggc ttcggtatcg gtgctgctgt ttctagctcc aaacgtactg
     1081 atgatcagaa cttcggcctg aacggttatg gtgagcgtta tttgggtaac ggcgaccgtg
     1141 ctgaaaccta cactggtggt ctgaaatacg acgctaacaa catctacctg gctgctcagt
     1201 atacccagac ctacaacgca actcgcgtag gttccttggg ttgggcaaac aaagcacaga
     1261 acttcgaagc tgttgctcag taccagttcg acttcggtct gcgtccgtct gtagcctacc
     1321 tgcagtctaa aggtaaaaac ctgggtgtca tcaatggtcg taactacgac gacgaagata
     1381 tcctgaaata tgttgatgtt ggtgcgacct actacttcaa caaaaacatg tccacctacg
     1441 ttgactacaa aatcaacctg ctggacgaca accgcttcac ccgtgacgct ggcatcaaca
     1501 ctgatgacat cgtagctctg ggtctggttt accagttcta atctcgattg atatcgaaca
     1561 aagggcctgc gggccctttt ttcattgttt tcagcgtaca aacacagttt tttggtgtac
     1621 tcttgcgacc gttcgcatga ggataatcac gtatggaaat aagctttacc cgcgtggcac
     1681 tgctggctgc cgcgctcttc tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc
     1741 acgctactga agttaccgtt cttgaaggca aaaccatggg taccttctgg cgtgccagca
     1801 tcccgggcat tgacgccaaa cgcagtgccg aacttaaaga aaagattcag acccagctgg
     1861 acgctgacga tcagctgctt tcgacctata aaaaagattc cgcgctgatg cgctttaacg
     1921 actcgcaaag tttgtcgccg tggccggtaa gtgaagcgat ggccgatatc gtcaccacct
     1981 cgctgcgcat tggcgcaagg accgatggtg cgatggatat aaccatcggg ccgctggtga
     2041 atctgtgggg ctttggcccg gaacaacaac cggttcaaat tccgagccag gaacagatcg
     2101 atgcgatgaa agccaaaacc ggcttacagc acctgacggt cattaatcag tcgcatcagc
     2161 aatatctgca aaaagacctg ccggatttat atgtcgatct ctccaccgtc ggcgaaggtt
     2221 atgcggcgga tcacctggca cgcttgatgg agcaggaagg gatttcccgc tatctggtgt
     2281 cggtgggcgg cgcgctgaac agccgtggta tgaacggtgc aggccaaccg tggcgggtag
     2341 cgattcaaaa accaaccgat aaagaaaacg cggttcaggc cgtggtggat atcaacggtc
     2401 atggtattag cacctctggc agctaccgta actattacga actggacggc aaacgtcttt
     2461 ctcatgttat cgatccgcaa accgggcgtc ctatcgaaca caatctggta tccgtgacgg
     2521 tgattgcccc gacggcgctg gaggccgatg cctgggatac tggcttgatg gtactcggac
     2581 cggagaaagc caaagaggtt gttcgccggg aaggactggc ggtctacatg atcaccaaag
     2641 agggcgataa ttttaaagcc tggatgtcgc cgcagtttaa aagcttcctt atcagcgaaa
     2701 aaaattaaag cgcaagattg ttggtttttg tgcgatggtg accgggcaga ctaaaggcta
     2761 tccttaacca gagagctgat tatgaaatac gccatacgct taactgacga tcaacgctgg
     2821 caatctgtct tagcccgcga ctcgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc
     2881 acgggtatct tttgccgtcc gtcttgccga gccagacatg ccttgcggga aaatgtctcc
     2941 ttctacgcaa atgccagcga ggcgcttgcc gctggctttc gtccctgcaa acgttgtcag
     3001 ccagacaaag ccaatcccct gcaacatcgc cttgataaaa ttacccacgc ctgccgactt
     3061 ctggaacagg aaacgcctgt aacgctggaa gccttagccg accaggtggc gatgagtcca
     3121 tttcatttgc atcggttgtt taaagcgact accggaatga cgcctaaagc ctggcaacag
     3181 gcctggcgcg ctcgccgttt gcgcgaattg ctggcgaaag gggagagcgt gactacatct
     3241 attcttaacg ccggattccc cgacagcagc agttactacc gcaaagccga cgaaacgctg
     3301 ggcatgacgg ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg
     3361 gctgattgtg agctgggccg ttgcctggtg gcagaaagtg agcgggggat ttgcgcgata
     3421 ttactgggcg atgatgacgc gacactaatc agcgagttac agcagatgtt tcccgctgcc
     3481 gacagcgcgc ctgctgatct gacgtttcag caacatgtgc gtgaagtgat cgccagcctc
     3541 aatcaacgcg atacgccgct gacgttaccg ctggacattc gcggcactgc ttttcagcaa
     3601 caagtctggc aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg
     3661 gctaacgcca tcggcaaacc gaaagcggta cgggctgttg ccagcgcctg tgccgccaac
     3721 aagctagcta tcgttatacc ctgtcatcgg gtagtacgcg gtgatggatc gctttcaggt
     3781 taccgctggg gcgtgtcgcg taaagcgcaa ctgctacgcc gcgaagctga aaatgaggag
     3841 aggtaatgtt ggatctgttt gccgatgctg aaccgtggca agaaccactg gcggctggag
     3901 cggtgatttt gcaccgtttt gcttttaacg ctgcggagca actgatccgc gatattaatg
     3961 acgttgccag ccagtcgccg tttcgccaga tggtcactcc aggcggatat accatgtcgg
     4021 tggcgatgac caactgtggg catctgggct ggacgaccca tcggcaaggt tatctctatt
     4081 cgcccattga tccgcaaaca aataaaccgt ggcccgccat gccgcagagt tttcatcatt
     4141 tatgtcaacg tgcggctacg gcagcgggct atccagattt ccagccagat gcctgtttga
     4201 tcaaccgcta cgttcctggc gcgaaactgt cgctgcatca ggataaagac gaaccggatc
     4261 tgcgcgcgcc aattgtttct gtttctctgg gcttacccgc gatttttcaa tttggcggcc
     4321 tgaaacgaaa tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg
     4381 gtggtgagtc gcggctgttt tatcatggca ttcaaccgtt gaaagtgggg gggcatccac
     4441 tcaccaccga ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat
     4501 aagaattatt attgctgtgc gcacgaagat gtttaaactg cgggctgtaa ttcattgtcc
     4561 gggttttctg catggaactt cttgtacttg tctggcggca gtatcgctgg ccatttatca
     4621 gtgtgatggc gctaagcctc gccagtgcgg cattaggcat tggcttaatt gcttttatca
     4681 atcagcgcct tatcgaaacg gcggatacca gtctgctggt attgccggag tttctggggt
     4741 tattgctgct gttgatggca gtcactctcg gatcgcaact ggcgctcacc actttggggc
     4801 atcacttcgt ttaccgactg cgtagtgaat ttatcaagcg gattctggat acccatgtcg
     4861 agcgcattga acaacttggt agcgcctcgt tgctggcggg gttaaccagc gatgtgcgca
     4921 atatcaccat tgcttttgtg cgtctgccgg aactggtgca ggggatcatt ctcactatcg
     4981 gttcagcggc gtatttgtgg atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga
     5041 tggcagtcac catctggggt ggttttatac tggtggcgcg ggtgtacaaa catatggcga
     5101 ccctgcgtga aaccgaagat aagctgtaca cggattttca aaccgtactc gaagggcgca
     5161 aagagctgac tctgaaccgt gaacgcgccg agtatgtgtt taacaacctc tacattcctg
     5221 atgcgcaaga gtatcgccat catattatcc gcgcagacac cttccatctt agtgccgtga
     5281 actggtcaaa catcatgatg ctgggcgcaa tcggcctggt gttctggatg gcgaacagtc
     5341 tcggttgggc tgataccaac gttgccgcga cctattcgtt gacgttgtta ttcctgcgta
     5401 cgccgctgct ttcggcggtt ggcgcgttgc cgacgctact gacggcgcag gtggcgttta
     5461 ataagctgaa caaattcgcg ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt
     5521 ttcccaactg gcaaacgctg gagctgcgta acgtgacgtt tagctatcag gataacgcgt
     5581 tttccgttgg tccaattaat ctcaccatta aacgtggcga gctgctgttt ctgattggtg
     5641 gcaacggtag tggaaaatcg acgctggcga tgttgctgac gggcttgtac cagccacaaa
     5701 atggcgaaat cttgctggat ggcaaacctg tcagcggcga acaaccggaa gattatcgaa
     5761 aactgttttc ggcagtgttt accgatgtct ggctgtttga tcaactgctg gggccggagg
     5821 ggcaacccgc taacccgcaa ctggttgaga agtggctggc gcagctgaaa atggctcata
     5881 agcttgagtt aagcaacggg cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac
     5941 gcgtggcgct gttgctggcg ctggcagaag aacgcgatat tatcctgctg gatgaatggg
     6001 cggcggatca ggatccacac ttccgtcgtg agttttatca ggtgctgctg ccgctgatgc
     6061 aggagatggg taaaactatt ttcgccatca gtcatgatga tcattacttt atccacgccg
     6121 accgcctgct ggaaatgcgc aacgggcaac ttagcgagct gacaggcgaa gagcgcgatg
     6181 ctgcttcgcg tgatgccgtt gcccggac
//
LOCUS       DAOFSV010000204         6193 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.204, whole genome shotgun sequence.
ACCESSION   DAOFSV010000204 DAOFSV010000000
VERSION     DAOFSV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.204"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            113..2557
                     /gene="dmsA"
                     /locus_tag="QBH27_004382"
     CDS             113..2557
                     /gene="dmsA"
                     /locus_tag="QBH27_004382"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDQ7016613.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            2568..3185
                     /gene="dmsB"
                     /locus_tag="QBH27_004383"
     CDS             2568..3185
                     /gene="dmsB"
                     /locus_tag="QBH27_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDQ7016614.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            3187..4050
                     /gene="dmsC"
                     /locus_tag="QBH27_004384"
     CDS             3187..4050
                     /gene="dmsC"
                     /locus_tag="QBH27_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDQ7016615.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFISSMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCVWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAN"
     gene            complement(4086..4712)
                     /locus_tag="QBH27_004385"
     CDS             complement(4086..4712)
                     /locus_tag="QBH27_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ7016616.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            5026..6174
                     /locus_tag="QBH27_004386"
     CDS             5026..6174
                     /locus_tag="QBH27_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016617.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         1445 a         1461 c         1689 g         1598 t
ORIGIN      
        1 gattttcaaa ttattcgatg tatacaagcc tatatagcga tctgctatag aaataattac
       61 acaatacggt ttgttactgg aatcaatcgt gagcaagctt gagtgagcca ttatgaaaac
      121 gaaaatccct gatgcggtat tggctgctga ggtgagtcgc cgtggtttgg taaaaacgac
      181 agcgatcggc ggcctggcaa tggccagcag cgcattaaca ttacctttta gtcggattgc
      241 gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa aaggttatct ggagcgcctg
      301 tacagttaac tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg gtgaaatcaa
      361 atatgttgaa acggataata ctggcgatga caactatgac ggcctgcacc aggtgcgcgc
      421 ctgtctgcgt ggtcgttcca tgcgtcgccg cgtttacaat cccgaccgcc tgaaatatcc
      481 gatgaaacga gtcggggcgc gcggtgaagg caaattcgag cgcattagct gggaagaagc
      541 ctacgacatc atcgcgacca atatgcagcg cctgatcaaa gagtacggca acgaatccat
      601 ctatctgaac tatggcaccg gtacgctggg tggcaccatg acccgctcct ggccgccggg
      661 aaataccctg gtcgcgcgcc tgatgaactg ctgcggcggt tatctgaacc attacggtga
      721 ctactcttcc gcgcaaattg cggaaggttt gaactatacc tacggcggct gggcagatgg
      781 taacagcccg tcggatatcg aaaacagtaa actggtagtg ctgtttggta ataaccctgg
      841 cgaaacgcga atgagtggcg gtggggtgac ttactatctt gaacaggcgc gccagaaatc
      901 taatgctcgc atgatcatca tcgatccgcg ctataccgac accggtgccg ggcgcgaaga
      961 tgagtggatc cctattcgtc cgggaacaga tgccgcactg gttaacggtc tggcgtatgt
     1021 catgatcacc gaaaacctgg tggatcaggc attcctcgat aaatattgcg ttggctacga
     1081 cgagaaaaca ctgccagcca gtgcgccgaa aaatggtcac tataaagctt atattctggg
     1141 tgaagggcca gatggcgtgg ctaaaacgcc ggaatgggcc tcgcaaatca ctggtgttcc
     1201 ggcagacaaa atcatcaaat tggctcgtga aatcggtagt accaaaccgg cgtttatcag
     1261 ccagggatgg ggcccgcagc gtcacgctaa cggtgaaatc gcaacccgtg ctatctcgat
     1321 gctggcgatt ctgaccggta acgttggtat taacggaggc aacagcggcg cgcgtgaagg
     1381 ttcatacagc ttaccgtttg tccgtatgcc gaccctcgaa aacccgatcc agaccagcat
     1441 ttcgatgttt atgtggaccg atgccattga acgtggcccg gaaatgacag cgctgcgtga
     1501 tggtgtgcgc gggaaagata agctggatgt gccgatcaaa atgatctgga actatgccgg
     1561 taactgcctg attaaccagc attctgaaat caaccgtacc catgaaatcc ttcaggatga
     1621 taagaagtgc gagctgattg tggttatcga ctgccacatg acctcatcgg cgaaatatgc
     1681 tgacatcctg ctgcctgact gcaccgcttc agaacagatg gactttgcac tggatgcatc
     1741 ctgcgggaat atgtcttacg tgattttcaa cgatcaggtg attaaaccgc gctttgaatg
     1801 taagaccatc tatgaaatga ccagcgaact ggcaaaacgt cttggcgttg agcaacagtt
     1861 tactgagggc cgtacccagg aagagtggat gcggcatctg tatgcccagt cgcgggaagc
     1921 gattcctgaa ctgccaacgt ttgaagagtt ccgtaagcag gggattttta aaaagcgcga
     1981 cccacaaggg catcacgttg cttataaagc tttccgtgaa gatccgcagg caaatccact
     2041 gaccacgcca tcggggaaaa ttgagattta ttcgcaggcg ctggctgaca ttgccgctac
     2101 ctgggaattg ccagaaggtg atgtgatcga tccactgccg atctatacgc cgggttttga
     2161 aagttatcag gatccgctga acaaacagta tccgctgcag cttacgggtt tccactataa
     2221 atctcgcgtt cactcaactt acggcaacgt tgatgtgctg aaagcagctt gccgtcagga
     2281 aatgtggatc aacccgcttg atgcgcaaaa acgcggtatc aacaacggcg ataaagtcag
     2341 gatctttaac gatcgtggtg aggttcatat tgaggcgaaa gtgacgccac gaatgatgcc
     2401 gggtgtggtc gcactgggtg aaggtgcctg gtatgacccg gatacaaaac gtgtcgataa
     2461 gggcggttgt attaacgtac tgaccactca acgtccgtct cctctcgcta aggggaatcc
     2521 gtcacataca aaccttgttc aggttgaaaa ggtgtaagga gtaaccgatg acaacccagt
     2581 atggattttt tattgattcc agccgttgca ccggttgcaa aacctgcgag ctggcttgta
     2641 aagactacaa agatttgacg ccagaagtca gcttccgccg catttatgaa tatgctggtg
     2701 gcgactggca ggaagataac ggtgtctggc accagaacgt gtttgcctac tatctgtcga
     2761 tttcatgtaa ccactgcgaa gatccggctt gtactaaagt ctgcccgagc ggtgcgatgc
     2821 ataaacgtga agatggtttt gttgtggtcg atgaagatgt atgcattggc tgccgttact
     2881 gtcatatggc ttgcccgtat ggcgcaccgc aatataacga aacgaaaggc catatgacca
     2941 aatgcgatgg ttgttatgac cgtgttgcgg agggaaaaaa accgatctgc gtagagtcct
     3001 gcccgctacg ggcgctggat ttcggcccta tcgacgagct gcgtaaaaaa catggcgatc
     3061 tggcggccgt tgcgccgttg ccgcgagctc actttaccaa accgaatatt gtgatcaaac
     3121 ccaatgccaa cagccgccca accggggata ccactggcta tctggcaaac ccgaaggagg
     3181 tgtgagatgg gaagtggatg gcatgaatgg ccgctgatga tcttcacggt cttcgggcaa
     3241 tgtgtcgcgg gtggttttat cgttctggct ttggcgctgc tcaaaggcga cctgcgagca
     3301 gaagcccagc agcgtgttat cgcctgcatg ttgggtttat gggtgctgat gggcattggc
     3361 tttatctcct ctatgcttca tcttggttca ccgatgcgcg cttttaactc gctcaatcgg
     3421 gtaggggctt cagcactcag taacgaaatc gccagcggtt cgatcttttt tgctgtaggc
     3481 ggcatcggct ggttgctggc aatgctgaaa aagctttcac cggcattgcg tacgctttgg
     3541 ctggtggtga cgatggttct tggcgtagtc tttgtctgga tgatggtgcg tgtgtataac
     3601 agcatcgata cagtacccac ctggtacagc atctggacac cgatgggctt cttcctgacg
     3661 atgtttatgg gcggcccgtt actgggttac ctgttgctga gtctggctgg cgtcgatggc
     3721 tgggcgatgc gtctgctgcc agccatttct gttctggcac tggtagtgag tggcgtgatg
     3781 tcagtgatgc agggcgcaga gctggcgact attcatagct ctgtgcagca ggccgcagcg
     3841 ctggtgccgg actatggtgc gctgatgtcc tggcggatcg tgcttttggc cgttgccctg
     3901 tgcgtgtgga ttgcaccaca gctaaaaggt tatcagcctg cggttccgct actttctgta
     3961 tcattcattc tgcttctggc aggggaatta atcggtcgtg gcgtattcta tggtttgcat
     4021 atgaccgtgg ggatggctgt cgcaaattaa tcagtaacaa ccggggtttc ggccccggtt
     4081 cgcttttatt tctgcttcgt taacgtgtca taactggtca tcaagttacg ataatcagga
     4141 atgtggttag agaacaacgt cgccagtcct tcaatatcat tacgccagtc gcgatgcagc
     4201 tcgcacgcca cgccgaacca tgtcatcaat tgcgcaccag cttgcgacat gcgatcccat
     4261 gccgaatgcc gggtaatttc attaaaggtc cctgacgcgt cagtcacgac aaagacgtca
     4321 aacccttctt caatggctga aagcgccggg aatgccacgc aaacttcggt taccacaccg
     4381 gcaataatta actgtttttt acctgtcgct ttgacagctt ttacaaaatc ttcgttatcc
     4441 caggcgttaa tatttcccgg gcgagcaatg taaggtgcat cggggaattg tgctttcagt
     4501 tctggaacta atgggccgtt aggaccggtt tcaaaactgg tggtaagaat agttggtaag
     4561 ttgaaatact tagccaggtc acccagcgcc agcacgttat ttttaaactt atcgggttcg
     4621 atatcccgta caagggaaag taaaccagcc tggtgatcaa caagcaaaac ggcagcatca
     4681 tttttatcaa gacgaacata cggtttggtc atcctcttct cctttcgaga gtcagtggta
     4741 cgtctaaatc ggtcagacgc gatgcattgc tctgaaagca tagacgggaa atatgagttt
     4801 gctgtgacca tgaaattttt cgactgaacg aagcgtatga aatttgtgtt agatcaataa
     4861 aaacaatcag atgagattaa tcgtctcttc agcattagcg ttatgaatga gtaatataaa
     4921 tcgataatac cgccgctgcg atatgcgcgt attttcaccc attgacaatg tttttggcgg
     4981 tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt aattcatgtc cacgtatacc
     5041 cggcctgtca tgcttttgct gtctggcctg cttttgttga ctctggcgat cgcggtgtta
     5101 aatacactcg tgccgctttg gctcgcccag gaacacatgt ctacatggca ggtaggcgtt
     5161 gtcagctcat cctattttac cggcaacctt gtcggtacat tgctgacagg gtatgtcatt
     5221 aaacgcattg gctttaaccg cagctattat ctggcctcct tcatttttgc cgctggctgt
     5281 gccggccttg gcctgatgat tggattctgg agttggttgg cgtggcgttt tgtcgctggc
     5341 atcggctgtg ccatgatttg ggtggttgtt gagagtgcgc tgatgtgcag tgggacgtcg
     5401 cgtaaccgcg ggcggttgct tgctgcgtat atgatggttt attacgtcgg aacgttttta
     5461 ggccagttac tggtcagcaa agtctcaacc gagttgatga gtgtattgcc gtgggttaca
     5521 ggtttgacgt tggcaggaat cttaccgctg ttgtttacgc gtgtcctgaa tcagcagact
     5581 gaaaaccatg attcgacgtc aattacgtca atgctaaaac tccgtcaggc gcggcttggc
     5641 gtgaatggct gcattatctc aggaatcgtg ctgggatctc tatatggcct gatgccgctg
     5701 tacctcaatc acaaaggggt gagcaatgcc agcattggtt tctggatggc ggtactggtc
     5761 agtgcgggta tcctcggaca atggccgatt ggacgtctgg cggataagtt tggtcgactg
     5821 ctggtgttgc gtgttcaggt ctttgtcgtc attctcggca gtattgcgat gcttagccag
     5881 gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg gctttacgct atatccggtg
     5941 gcgatggcct gggcttgtga gaaagttgaa catcatcaac tggtggcgat gaaccaggcc
     6001 ttactgttga gctataccgt gggaagcctg cttggcccgt catttaccgc tatgctaatg
     6061 cagaatttct ccgataattt attgtttatc atgatcgcca gcgtatcgtt tatctatttg
     6121 ctgatgctgc ttcgcaacgc cggccatacg ccgaaacccg tggctcacgt gtaaatgaat
     6181 tcaagcagag tgt
//
LOCUS       DAOFSV010000205         6167 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.205, whole genome shotgun sequence.
ACCESSION   DAOFSV010000205 DAOFSV010000000
VERSION     DAOFSV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.205"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(13..801)
                     /locus_tag="QBH27_004387"
     CDS             complement(13..801)
                     /locus_tag="QBH27_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ7016618.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(841..1737)
                     /locus_tag="QBH27_004388"
     CDS             complement(841..1737)
                     /locus_tag="QBH27_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ7016619.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            1910..2788
                     /gene="yihU"
                     /locus_tag="QBH27_004389"
     CDS             1910..2788
                     /gene="yihU"
                     /locus_tag="QBH27_004389"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDQ7016620.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            2813..3700
                     /locus_tag="QBH27_004390"
     CDS             2813..3700
                     /locus_tag="QBH27_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDQ7016621.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            3733..4743
                     /locus_tag="QBH27_004391"
     CDS             3733..4743
                     /locus_tag="QBH27_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDQ7016622.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            4813..>6167
                     /locus_tag="QBH27_004392"
     CDS             4813..>6167
                     /locus_tag="QBH27_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016623.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     T"
BASE COUNT         1450 a         1488 c         1625 g         1604 t
ORIGIN      
        1 gaagtttcaa tttcaggata cttcttgctc ggcaaccacc agttcaacat tctcttcact
       61 taacaactgg cagaaagaat tgggaggcgg ggcatcagta aaaaaagccc tgacattccg
      121 tgcattgcca attgaaacgg cagcagacgc ggtaaacttc gtatgatcgg ccactaacaa
      181 tgtattccgc gcatgtttaa tcatcgttct cgcgactaac gcttcattga catcaaattc
      241 cagtagggtg ccgtcatgtt caatggcacc tatactggtg attaaataat ctgcgcggaa
      301 gccttcaata aaatccacgg ctcctgggcc gataatccca ccgttatgag cgcgtaaagt
      361 gcctcccggc accatcactt caatatcctg attcttataa agaatctgtg ccacacgcag
      421 gctattagtg ataatgcgta aatcacgccg gttgagtaat gccctggcaa cggcttctac
      481 ggttgtaccg atggtgataa agacggtaca gcgttcggga aggtaatcgg cgactgcctc
      541 agcgatcgcc cgtttctcgg cggtgagcga aagttcccgt tgctcaaaag cggtattcat
      601 gacgctggag acgcgcccgg cccctccatg atgacgggtg atcaagccct gctcactgag
      661 ttttcggata tcccggcgca cggtttgcgt tgaaacatcc agcagttgtg ccagttcttc
      721 aatattcata taaccgcgtt ctgcgattag atgaacgagt tggtcatggc gtggattacc
      781 tgtcacttcg atgatactca tgaatactcc cttgcccctc ctcggtagat gtttaccatt
      841 ttacgcataa agtgacaaaa atgattcggt ttgctcacga ttaggaatgc cggcccgacc
      901 gccaggctgg gtacatttca tcgcggcaac cgcactggca aaacgaatag cctcttttgt
      961 tggcattttt tctgccaacg ctaccgcgag tgcgccgtga aaaacatcgc ctgctccagt
     1021 ggtatcaacc acattgacag aaaatgcctc ctgttgacac aaatgaccat cttcaatcca
     1081 caacgatcct tcagaaccca gcgtaacata cactttacct gctgtttgtg tcgttgcctg
     1141 gaacaaacct tcttcaggtg actgcaggcc agtcatgcgt ttcagccctg gtgttgagaa
     1201 cacggcgtga tcggcaagcg cgacaagtgg agaaatatcc tgcggtgtca tatcggcgtc
     1261 taaaagcgtt gtcacacccg caaggcgcgc tagcgagaat gctttttcag taccgctatg
     1321 ccagcgaaca tcggccagaa tcagatcata acgagagaaa tcgatcgctt ctaaccattc
     1381 ggcatcagta ccgagatcag gacttggata attaacgata atccgttcgc catgctgatc
     1441 aacgaggatc gctgattgcg acgaacgggc gttaggatat cggcggcaaa atgcggtatt
     1501 gacgccccaa ccctcaagtt ctgcaagtaa ggtatttcca caactgtcat cgccgacgcg
     1561 accgatgaaa tcaacctcca cgccaagctt tgcgattgca acagcggctg ttgctgccgg
     1621 gccgccacca atctcaagat aatgatttgc ctgatatttc ccgccaccct ccggcagagt
     1681 gggcaaagaa taaattctgt cctgaactgt gatacccacg catgcaattc tggtcatatt
     1741 tgtcttgtcc tgtttaattt tgtcgtcttt ggtcaattta aatgacacaa atgatcaaat
     1801 tagtgatgcg tgtcgtgttt ttgaacataa attacaaata tgctcaaaaa cagacgaaaa
     1861 cgaattttca attgtcatta ttttgtcaat gtcagacagg agagtattca tggcaaccat
     1921 tgcatttctc gggctggggc agatgggtag cccaatggct aacaatctat tgcaaaaagg
     1981 gcattcatta caggtatttg atgtcaatac acaggccgtc gatgcgctag ttacgcaggg
     2041 ggccactgcg gcacaaaccc cagcggaagc ggcagcaagc gctgaattta tcatcactat
     2101 gcttcccaat ggggacattg ttcgacaggt gctgttgggg gaaaaaggcg tctgtgaaac
     2161 tgtttcatct gacgcgttag tgattgatat gtcgactatt catcccttac agaccgatgc
     2221 attaatacgg gaattacaag aaaaaggcat caatatgatg gacgcccccg tgggcagaac
     2281 gtcagtgaat gcgattgatg gcacattgct gattttagcg ggcggcactg atgagcagat
     2341 tgcgcgtgca cgcccgattc tcatgtgcat gggtaatgag ctggtcgagg ctggtggccc
     2401 aggaatgggg atccgcgtga agctaattaa caactatatg agcattgcgc ttaatgcgct
     2461 gtcctccgaa gccgctgtgt tatgtgagtc actgggatta aatcttgatg tcgcaattaa
     2521 ggtgatgagt ggtacggcag cagggaaagg acattttacg accacctggc ctggcaaagt
     2581 gctgaaaggc gatctttctc ccgccttcat ggtggacctg gcattgaaag atctgcgtat
     2641 tgcagttgac gtcgctcgga aaacgggggc gccgctcaat atggggattg cagcagaaag
     2701 ttattacgcc gcagcaagtc aagacggcaa agggcggcag gactggagcg ccctgctaaa
     2761 tcaggttcgc cagcaggctg gtctttaatt ttatctatta acaggagttt ctatgtcatc
     2821 ccacttcacc ccatttacgc tgaaagatat ctcccgccca ggtggtggat tcgccatgtt
     2881 agctgtcgat cagcgtgagg ccatgcgcct gatgtttgcg gcagcagggc agccaaaacc
     2941 catcgccgac agtgtactca ccgattttaa agttgccgcg acccgaatcc tttctcctta
     3001 tgcttctgcc gttctggcgg ataaacaatt ttgtcttgaa caaatagtcg agcagggcgc
     3061 tgtggctaat tcttgcggcc tgattgtcgc tgccgatctt tttatcccag gtaatggcat
     3121 cccggttgat agcgttgaaa tagatatgtc cgtcgacccg cataaagccc gtgaaatggg
     3181 ggctaaggca atgaaattac ttgtactctg gcgtgaagat gaacccgcag aggaacgtct
     3241 ggcaatggtt gataaatttg ttcgtcgctg ccgtagtgcc gggctggtta gcattattga
     3301 acctgtggtg cgtccacctc gtcgcggttg ggactttgat cgtgaaagcg ccattgttgc
     3361 cgccgccgca gagcttggtg gaacagaggc tgatctttac aaagccgaga tgccattagg
     3421 gggcaaaggc gatgaaaaaa ccttacttgc tgcctgtcag caacttaacg atcagatgaa
     3481 gatgccgtgg gtaattcttt cttctggcgt tgatgccgat atttttggcc gtgcagtaag
     3541 tattgccatg aaaggtggtg cgtcgggctt cctggctgga cgagcggttt gggcgtcggt
     3601 tgttggtgca caagatccac aaaccatgct gcgcgatgtc tccgttccac gcttgcaacg
     3661 gctggctgaa attgtcgatg aaggtatcgc gcaacgctaa tcccctatgg atatcgattc
     3721 tggagaataa caatggataa aattacccag gttttatttt cagatattgg gaaagtcacc
     3781 actcaatatg ttgaagtacc acaccagcaa cttaaaccgc acgaagtacg gattgcgccc
     3841 gtgttctacg ggatatgcgg ttcggatctg catgttctga aaggcggtca tccgtttgcc
     3901 aaaccgcctg tcgtccccgg tcatgaaatt gcagcgcgcg ttacggaagt gggcagcgac
     3961 gttaaaaatg tacagccggg cgatcatgtt gtggtcgatc ccatcatggc ttgcatggaa
     4021 tgccgagcct gcaaagcagg acgttttaat ctttgtgaac caccgcaggt tgctggtttt
     4081 cgcgcaccgg gctttgctcg ctcacaacac attgttcctg cgcgtaattg ccatgtcgca
     4141 ccagcctctt taccgctaaa agtgttggcc tttgccgaac cggcggcttg tgctcgtcac
     4201 tgcgttaacc gaatgccgaa agcttctctg gaaagcgtac tggtgattgg tgccggaacg
     4261 ataggcttat ccatcgtgca ggcactgcgc attatggggg caggtaagat taccgtgatt
     4321 gaacctgacg ctgccaaacg cgcgctggcg ttaaaactgg gcgcagcaga agtttgggca
     4381 ccaggtgagc tggccgcaga tgtgcgattt acgggggcga ttgatgtcgt tgcagcgcag
     4441 gccacgctta acgatgcatg tacccgtgta tatgccggag gcaccgtcgt gtgcatgggc
     4501 gtaccaagtg ggccgcgtga aataccatta ccgatgatgc aacgtttcga gcgtgacttg
     4561 ctcaactctg gcatgtacat ccctgaagat ttcgatgctg ttatcgaatg gctggcggat
     4621 gggcggtttg ataccagtga actggttacc gatttatttg ccattgagga tgcagcggcg
     4681 gcattcgaac gcgcgcagca aaatgactcc ataaaggtca tgctgcaatt tgcgccggaa
     4741 tgattttcta aatctctgtc tggcgctgcg tcacgtcagc gccaccctac aacaattaaa
     4801 aaggacactg cgatgagtca aatcaccagt cctgcaactt attcaattag tcggccacag
     4861 gacgtaattg atattgttaa taagaactct gcaatcaaca ccagtatcgg cgttattttt
     4921 attgctttgg gcggcatatt gattgatgcc taccaggcgg cgatggtggg gtttggtaat
     4981 aaatacattg ccgctcaatt cggcatttcg ccgggtcttg ctgcaaccgt caatgcatca
     5041 gtattaatcg ccgcgttaat tggcggttta ttagcgaacc gagtaataaa ccgctttggg
     5101 caaaagcggg catttattat tggcatgggg ctgtgcacca tcggtgctgc agcggtagct
     5161 attgctccca gtatctggtg ggtgctggtg tgccgcgtca tcatgggctt tggtttaggc
     5221 atcgacttcc ctttggcaac caatgccgtg gcagagcttc gtggttcaac gtcgaagaaa
     5281 accggaacgt cggtcaacct ctggcaaatg gcctggtatg tttcgacaac cgttgtttat
     5341 ttggtgcttt tgccgctgct tctgtcgggt atcgctgaag aacaattgtg gcgttacgga
     5401 atattcatcg gagctatttt tgcagtcatc ttcatgattt tgcgttactt ctttattggt
     5461 gaatccgcaa tgtgggccgc acgcgtcggg cgttaccagg aagcgtgcga cattctggga
     5521 aaacgttatg gtgttcaggc tcgcgttgcg gcatcgggta caacagaagc gaaattctcg
     5581 gaaaaagcag agaataaata cagtggtgga tatggcatct tatttaatga tcgttaccgc
     5641 aaacgcacca ttcttggctg tgtcgtggca accatgcagg cgtggcaata taacgccgta
     5701 ggtgtttatc ttcctcttac gttggcggga ataataagtg gcgggcttac tggcgcgttg
     5761 accggttctg ccgtcgtgaa tgccctttgt ggggtgacag gcgggatgat cggctcgttt
     5821 attctccaac gactgggtac tcgccgacag tcgatgtatg gatttgctgt tgtgacctta
     5881 gcattgctgt cgttaggcgc actggcaacg actaatccct ggctgtcttt agggttattg
     5941 ggatcaatta ttttcttcca ttcagcgggt cctggtgggc tgggcatgac cattgccaca
     6001 ctctcttatc ctcccgctat tcgccctact ggggtcggat ttgcccgcgc tattatgcgc
     6061 acaggggcaa ttgcaggact cattttctgg ccgatgctgt ggggtgcgtt gaaaaccgaa
     6121 gcgttttact ggttggcaat cgtgccattc ctgggattcc tgacctg
//
LOCUS       DAOFSV010000206         6000 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.206, whole genome shotgun sequence.
ACCESSION   DAOFSV010000206 DAOFSV010000000
VERSION     DAOFSV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.206"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..523
                     /locus_tag="QBH27_004393"
     CDS             <1..523
                     /locus_tag="QBH27_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDQ7016624.1"
                     /translation="LNNKMLPNQCALLPLSKLIIISDNFRKLAKNADYNGPELFTHAH
                     QFVGQNYLGFGDYTITGQVLDLGGGQPSAVAAHLVFKNLQANTVWIRHFVSSNTQRGS
                     SNVTAKFLDVSDQITNLVPQHPTQFGSNIGLNYYYYNSQPTVRHFPGLPKNKQYQITH
                     HICFMLDLIAGRI"
     gene            complement(503..1678)
                     /locus_tag="QBH27_004394"
     CDS             complement(503..1678)
                     /locus_tag="QBH27_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ7016625.1"
                     /translation="MLLSDIQIKRAKPKDKPYTLNDGMGLSLLIDTAGSKGWRFRYRF
                     AGKPKMISFGVYGDVSLAQARTKRDEARSMLANGINPSEARKADKIALQFAHENSFES
                     VAREWHSSKKATWSEGYAKEVLNCMEKDIFPFIGQRPIEQIEPLELLTVLQKIEKRGA
                     LEQTSKIRRRCGEVLRYAVATGRAKYNFAPDLAIALNKPKTQHFPFLTESELPEFVNA
                     LDNYQGSLVTKYATQLLMLTGVRTIELRAAEWAEFDLDNALWEIPKERMKKRRPHLVP
                     LSTQAINILKKLQEITGNYSLVFPGRNDVSKPMSEASINKVIKLLGYHGRLTGHGFRH
                     TMSTILHEHGFESAWIEMQLAHVDKNSIRGTYNHAQYIKDRQVMMEWYASLLNTTCY"
     gene            complement(1839..1913)
                     /locus_tag="QBH27_004395"
     tRNA            complement(1839..1913)
                     /locus_tag="QBH27_004395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1878..1880),aa:Arg,seq:cct)
     gene            complement(1989..2921)
                     /locus_tag="QBH27_004396"
     CDS             complement(1989..2921)
                     /locus_tag="QBH27_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDQ7016626.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3215..3970
                     /gene="mlaA"
                     /locus_tag="QBH27_004397"
     CDS             3215..3970
                     /gene="mlaA"
                     /locus_tag="QBH27_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDQ7016627.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4032..5372)
                     /gene="fadL"
                     /locus_tag="QBH27_004398"
     CDS             complement(4032..5372)
                     /gene="fadL"
                     /locus_tag="QBH27_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDQ7016628.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSNLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            5744..>6000
                     /locus_tag="QBH27_004399"
     CDS             5744..>6000
                     /locus_tag="QBH27_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDQ7016629.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAE"
BASE COUNT         1560 a         1402 c         1374 g         1664 t
ORIGIN      
        1 tttaaataat aaaatgcttc ctaatcagtg cgctcttctt cctttgtcga agttaattat
       61 tattagcgat aactttagaa agttagcaaa aaatgctgat tataatgggc cggaactttt
      121 cacgcacgca catcaattcg tgggacaaaa ttacttagga tttggcgatt atacaattac
      181 tggccaggta ctagatcttg gaggtggtca accttctgct gttgccgcac atttggtgtt
      241 caagaattta caagcgaata cagtttggat tcgtcatttt gtatcaagca acacgcaacg
      301 aggaagttca aatgttactg caaaattttt agatgtttca gatcaaatta caaatcttgt
      361 tccccaacat ccaacccagt ttggcagcaa cattggtctt aactattatt attacaatag
      421 tcaaccaact gtaaggcatt tccctgggtt gccaaaaaac aaacagtatc aaattactca
      481 ccatatttgt ttcatgctgg atttaatagc aggtcgtatt taataagcta gcgtaccatt
      541 ccatcattac ttgtcgatct ttaatatatt gcgcatgatt gtaagtacct cgaatagagt
      601 tcttatcaac atgcgcaagc tgcatttcaa tccaggcact ttcaaaccca tgctcatgca
      661 agatggtgct catggtatgc ctgaaaccgt gacccgttaa ccgaccgtga taacccagta
      721 gttttatcac tttgttgatg ctggcttcgc tcattggttt actgacgtca ttccggccag
      781 ggaaaacgag gctgtaattc cctgttattt cttgcagctt tttcaggata ttgatcgcct
      841 gagttgataa cggaaccaga tgggggcggc gtttcttcat ccgttctttg gggatctccc
      901 ataaggcatt atctaaatca aactcagccc attccgctgc acgtagttca attgttcgca
      961 cgcctgttag catcaacagc tgcgtggcgt atttggtaac taaactgccc tgatagttat
     1021 ccagagcatt aacgaactcg ggcaactcgc tttcagtcag gaacgggaag tgttgggttt
     1081 taggtttgtt gagtgcgata gctaaatcgg gtgcaaaatt atactttgct ctaccagtgg
     1141 caacagcata acgaagcact tcaccacagc ggcggcggat cttactggtt tgctctagcg
     1201 cacctctttt ctctattttt tgcaggacgg ttagcagttc taatggttca atctgctcaa
     1261 ttgggcgctg gccgataaaa ggaaaaatat ccttttccat acaattgaga acctcttttg
     1321 cataaccctc tgaccaggtg gcttttttcg atgagtgcca ttctctggcc acagactcaa
     1381 agctgttctc atgcgcgaac tgcaaagcaa tcttatctgc ttttctggct tcacttgggt
     1441 ttatcccgtt ggctaacatc gaacgcgctt catcacgttt ggtacgtgcc tgtgccaacg
     1501 acacatcgcc atatacgcca aaggaaatca tcttaggctt acctgcaaag cgatagcgaa
     1561 aacgccagcc tttactgccc gctgtgtcga tcaggagtga tagccccata ccatcgttaa
     1621 gcgtataggg tttgtccttc ggttttgctc tttttatttg gatgtcagat agcagcatgt
     1681 gtatagaaaa aagatcgaac tcagttatac acaatgttat acgcaaagat gtatagattc
     1741 cattagaaat aatggtactt tgctggacga gtataaggag atgatatcag ttaaaacagt
     1801 gagttatgga attaattgaa cttagtgaga agtgttcatg gtgtcccctg caggaatcga
     1861 acctgcaatt agcccttagg aggggctcgt tatatccatt taactaagag gacaatgcgg
     1921 catgagtata cccgctaatg gactacgggg taagtacaca gccgctcgat tgcttaaacc
     1981 ctcgccattt atgccgggtt tttatcattt ttcttaatgt tttccgcacg ttctgctttt
     2041 tgacgtgctt ctgctttgcg cttgttgctc atgtcgttac gaatctgtgc atgactcatt
     2101 aacgcgaaga taaaggtgcc gccgcagatg ttccccgcta aagtaggtag tgcgaagggc
     2161 cagatgaaat cgctccagtg cagcgtgccg ttaaacacca gatagaggat ttcaacagaa
     2221 ccgacaacga tgtgggtggt gtcacccagg gcaataagcc aggtcatcaa tataatcacc
     2281 acaatctttg ccgcacccgc tgcaggaaac atccatacca tagtggcgat cagccagccg
     2341 gaaataatcg cgttggcaaa catctcgctg ggggtgttct tcatcacatc catgccgatt
     2401 ttgacaaatg catcgcgagt ttcttcattg aagataggca tatattcaaa tgcccacgca
     2461 gcaatacctg tcccgagaat attacccagc agcacgatgc cccataaccg cataagtaag
     2521 ccgacgttgc tcattgtcgg tttttgcatg acgggtagta ccgcagttac ggtgttttcg
     2581 gtaaataatt gctggcgggc cattatgacg ataataaaac caaaggtata accgagattc
     2641 tccagcaaga agctgcccgg cacgccttcc agttcgacat gaaatatccc ttttgccagt
     2701 aacgaagcgc ccatcgacag gcccgccgca atggctgacc acagtagcgc cattgcgtcg
     2761 cgttccagct ctttttcacc atcctggcgg atatgctcat gaattgccat cgcccgggag
     2821 gggagtcggt cttcatctat ttctattttt ttaccgcgct ctttttcttc gctctcaact
     2881 tcaatttcgt cgctgtgttg atcaattttg tcgttgtcca tggtctcttc tcttgaatta
     2941 gcacgtatag ctaaagcgta gcggcttttt tgctcgcaac tggcgggagt tactctgaaa
     3001 atgtagaaaa ggccgcgttt gcctttttct gtttctattg aatcaagtag cctacagggc
     3061 ggcgattacc aggctatgat caaatcagca aatcagggcg tctggacatc agttgacgtg
     3121 ctgttacaat cgcccacacc taaacaggcg gatacggtat cgttccgtca tggatggcaa
     3181 actgcataag ccataaaaaa acagggagac atttatgaag cttcgcctgt cggcgcttgc
     3241 tctgggaact acgcttctgg tggggtgtgc gagttccggt acagatcagc aagggcgttc
     3301 tgacccgtta gaagggttta accgcactat gtacaacttc aacttcaatg tattagaccc
     3361 gtatattgtt cgaccggtcg ctgtcgcctg gcgtgattat gttccgcaac ctgcgcgtaa
     3421 cggtttgagc aactttaccg gcaaccttga agaacctgcg gtgatggtta actacttctt
     3481 gcagggcgac ccttatcagg ggatggtcca ctttacccgc tttttcctga ataccatttt
     3541 gggaatgggc ggctttattg atgttgcagg gatggcgaac ccgaaactgc aacggactga
     3601 acctcaccgc ttcggtagta cgcttggtca ttatggtgtg ggttatgggc cgtacgttca
     3661 gttaccgttc tacggtagct tcacgctgcg tgatgacggt ggtgatatgg cggatagtct
     3721 ttacccggtt ctttcctggc tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg
     3781 gatagaaact cgtgcgcagt tgttggattc cgacggtctg ctgcgtcagt cctccgatcc
     3841 ttatattatg gtgcgtgaag cgtacttcca gcgtcatgat ttcatcgcta atggcggtga
     3901 actcaaaccg caggaaaatc cgaacgcgca agcgattcag gatgatttaa aagatatcga
     3961 ttctgaataa gaaacaaaca aaaaaggtga gtcgcaaaac tcaccttttt tcttattaag
     4021 ctaacgcgtt atcagaacgc gtagttaaag ttagtaccga acagccaggc tttaccttca
     4081 gactcgaagg aataagctgc cgctccttca ccttcggtga atttcacttt ctgaccatgc
     4141 atataagatg cgccaacatc aatggatgca tcttcattaa atgcgtaggt taacccagca
     4201 cttaaccaca aacgatcctg gtcaggaatg gaaatagaac gtttatctgc cggaaccggg
     4261 ctgtcgtcat aagcgatacc ggtacggaag gtccagtttt tatcataata gtaggttgta
     4321 cccaacgcga tacgatacgc gtcccggaat ccttcatctt tatagaacag agtctgacca
     4381 ctatcgcctt tcgctttcaa ttcctggaac tggctccaac tggtataggc catgctgtag
     4441 tgaattgccc actgcggcgc aacacggtta taaccagaaa tctcccacat ctcaggcagg
     4501 ttcagcgtta aggatccatt ttgggttgcc cccatcgtgc catacggtaa gcccatgtta
     4561 cccaaaatat tgttcagatt tgggttaatg gagcttttgt aatccccatc gaagtcgatt
     4621 ttaacttctg aacgatatgt cagcccccaa cggttatctt tatccagttc ataaagaata
     4681 cccgcgttcc agccaaagcc ccattcgtca cctttcaaat gagcaatttg agagtccgga
     4741 gtattaatac cattggttaa tgaagcgggt acaccctggg caatcaactg ttgagacaac
     4801 aatggcccca gcgaaccggc gtaacgctca atttttgcct tggcataaac tgcatcaaaa
     4861 ccaagaccaa aactccagct attgttaagg cgataagccc cgctaaggtt aaggttgatt
     4921 gtttgcagat cggttttacc gccatagata ccagcagcgt agttgtcggt aaattccgta
     4981 gccaggccat agttggaagt tattgacgct ccccaaccaa actgatcgtt aataggcgcg
     5041 acaaagtgca ggttaggtac ccacgctgtc ggcgcgatat ttttagcatt tgcactctga
     5101 ccggttaaat tagaatgtcc ggtaatatct acatccggat caatataaac cgcacccgca
     5161 gaaaatgtcg ggcggtcaaa catggtaatc aatgcggggt tacggctaac gttacctgca
     5221 tcatcggcaa ttgcaccttc ccctgaataa gcccggccca ggccagagga agaaaattcg
     5281 tttaactgaa agcctgccga ccaggcctgg gtggagataa gtgccactgc gactgcgaga
     5341 gcagactttg taaacagggt tttctggctc atgaccataa cctcaatgat ttatttttat
     5401 acaaactatg ttacgtgctg taacaggagc gcgaagtgta gggtctgagg tacatcttag
     5461 aaatcagacc agtggcgaga gtataggtcg gaccagctgg gatgttgcaa gtatgtttct
     5521 atcttttttc agatatgatc taagaaacgc gatccagctg gcaaaattgg gaggtggcgc
     5581 actcatggat tcaggtgaat tttgttttag atcattttta agtgtgattt cggtcactta
     5641 tccgattttg cagaattaac aactggagca gcactttccg gaagcgtaaa atatccacaa
     5701 cgtttatctg gcaccttggg tgcacataca gaggaaatca acgatgagta aatgtagtgc
     5761 tgatgaaacc ccggtttgct gctgtatgga tgttggcacc attatggaca actccgactg
     5821 caccgcgtct tacagccgtg tgtttgcaaa ccgcgcagaa gcagaacaaa cgctggcggc
     5881 gctgactgaa aaagcccgta gcgtggaatc cgaaccgtgc aaaatcaccc cgactttcac
     5941 tgaagagtct gacggtgtgc gcctggatat cgatttcact tttgcctgcg aagctgaaat
//
LOCUS       DAOFSV010000207         5993 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.207, whole genome shotgun sequence.
ACCESSION   DAOFSV010000207 DAOFSV010000000
VERSION     DAOFSV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.207"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1875)
                     /gene="aceF"
                     /locus_tag="QBH27_004400"
     CDS             complement(<1..1875)
                     /gene="aceF"
                     /locus_tag="QBH27_004400"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDQ7016630.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     I"
     gene            complement(1890..4553)
                     /gene="aceE"
                     /locus_tag="QBH27_004401"
     CDS             complement(1890..4553)
                     /gene="aceE"
                     /locus_tag="QBH27_004401"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDQ7016631.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(4714..5478)
                     /gene="pdhR"
                     /locus_tag="QBH27_004402"
     CDS             complement(4714..5478)
                     /gene="pdhR"
                     /locus_tag="QBH27_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDQ7016632.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         1331 a         1618 c         1498 g         1546 t
ORIGIN      
        1 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
       61 cacgcggtgg tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc
      121 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcatt
      181 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctcgagatgg tgaagcaacc
      241 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
      301 ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg ggacaaccag
      361 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
      421 accgtcttcc gacagcgaac tgttgaagcg aggcatctgc tcaagagctg cagcaacggc
      481 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ctgcttcttc
      541 gttctgctgt ttacggaacg cttccagctc agtgatatcg gttttgtcga agtgagtaac
      601 gtgcgggatc atcacccagt tacggctcag gttagcacca gagattttct gaatgcggcc
      661 cagttccact tcttcgattt caccaaactt gctgaagtcc actttaggcc acggcagcat
      721 gcctgggata ccgccgccag ttgccgccgg agctgcttct gcacgtttga ttgcttcttt
      781 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
      841 cgccaggtta acgccaaact cgcgtgccag acggcggatc agcggagtcg cgtgaacgta
      901 agcgtcgttt tcagcaaatt cagatttgcc ttcggctttc gcagccggtg ctgctgccgg
      961 ggcttcagct tttgctgccg gagccggcgc tgccgcttcc tgtttcgcag gagctgccgc
     1021 aggcgctgcg ccttcaactt cgaagatcat aatcagcgaa ccagttttca ctttatcgcc
     1081 aacgttgact ttcagttcct tcacgacgcc tgcaaacgga gccggaactt ccatagaagc
     1141 tttatcgcct tctacggtga tcagtgactg ttcagcggca actttgtcgc ccactttcac
     1201 catcacttcg gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac
     1261 gccagctgct ggtgcagagg ccgctgccgg agccgcttcc tgtttagccg ccggagctgc
     1321 cgcgccagct tcacccgcga cttcgaagac cataatcagc gagccggtag acactttgtc
     1381 acccacgttc actttgatct ctttcacggt acctgcaaac ggagccggaa cttccataga
     1441 agccttgtcg ccttctacgg tgatcagcga ctgttcagct tcaactttgt cgcccacttt
     1501 gaccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt
     1561 tgccgctgca gccgctggtg ctgctgccgg agcggcttct ttcttctctt ctgcctgagc
     1621 aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg
     1681 ggttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagatggtac
     1741 ttccatagag gctttgtcgc cttctacggt gatcagcgac tgttcggctt caactttgtc
     1801 gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt
     1861 gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca
     1921 tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta
     1981 gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcaaa gtggtgacgc
     2041 aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg gtagtcgtca
     2101 gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccact
     2161 gccggagcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcgggtgc
     2221 agcatgttcc agcgttcaca atcctgacca tcacgcgcca gttcggtgaa ggaagtcacg
     2281 ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg
     2341 tgacgcagga tagaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt
     2401 tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg
     2461 tggtagtttt cgttcagcgt agtgatgtag tagtaaacgt tctcttgttt ttcaccgtac
     2521 atacgctcca gaccgtcatg catgatgaca gcaacttcgt aagcgtaagc cgggtcgtaa
     2581 gagatacagt tcgggatagt cagcgactga atatggctgt gaccatcttc gtgctgcaga
     2641 ccttcaccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc
     2701 tggtcgccag ccgcccagca cagatcgccg atacgctgga aaccgaacat cgagtaatag
     2761 atgtagaacg ggatcatcgg cagattgttg gtgctgtaag aggtcgccgc tgccagccag
     2821 gaacaacctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct
     2881 ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa
     2941 ataccaatct gacggaacag accttccata ccgaaggtac gcgcttcgtc ggcgatgatc
     3001 ggtaccagac gatctttgat cgacttgttc ttcagcatca cgttcagagc acgaacgaaa
     3061 gcgatagtgg tagagatctc tttgctctgc tcttccagca gcgcgccgaa atcttgcagg
     3121 ctcggcagct ccagcttctc ggtgaagttc ggctggcggc ttggcagata accgtgcagt
     3181 ttctgacgtt gagcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac
     3241 ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc
     3301 acgccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg
     3361 cccatgccgt aacctttaat ggtatgagca aggattaccg tcgctttgcc tttggtttcc
     3421 tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc
     3481 cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag
     3541 aagtgttcgc gaacatacgc accatctttc gatttgaagg tctggtaatc gccgtcaacg
     3601 gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag ttcgtcccaa
     3661 cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat gccttccagt
     3721 tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg
     3781 ataacgaaga ccagattatc cagtttttca cgggtagcga tggtgatcgc acctttggat
     3841 tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtttgttt agaggtatct
     3901 ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcgga
     3961 cccagcccca tagatacggt cgggaactgc cagaattccg gcatcagttt cgggtgcgga
     4021 taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcctgagtc
     4081 agacgacctt ccaggaaagc acgagcgtaa acgcctgggg agatgtggcc ctggaagtaa
     4141 accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca
     4201 taaatggttg cggaagactg gaaggacgcc atgtggccgc ccagttcgag gtcttttttc
     4261 gacgcacgca gcaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc
     4321 agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg
     4381 ctgataccgg tgcctgcagc tacgttgact ccgcctttgc gggcttcagc aagcagttgg
     4441 tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg
     4501 agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat
     4561 tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccgga
     4621 aagctcaata agacaggttc tacgtttagt tgccgcgctt ttatatgcgc ttgatttaca
     4681 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
     4741 cagagaacgc tcacggcggc tctcttcacg gcttctgtcg agcaaaattt cttcgataaa
     4801 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
     4861 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
     4921 ttcgaagttc tggcggacat tctgagccaa catcggctcc atacacctta gcagatgaag
     4981 cagaaccaca ttgtgggccg cttcggtgac ggcaatctga tactggagta cggcatttga
     5041 ttccgcgtcc agatcgccag actgctgcgc cagctctatg gcatggtgga gttcacggat
     5101 gcgttccttg tcttcatcgg tactacgcag cgcggcgtaa taagcggcga taccttccag
     5161 ggcgtgtcgt gtttcgagca agtcatactg tgattcagga tggtcggaga gcagctccac
     5221 cagcggatcg ctgaagcttt gccagaggct gctctggaca aaagtgccgc caccctggcg
     5281 acgaagcaac aagcccttcg cttcgaggcg ttgaatcgcc tcacgcaaag agggacggga
     5341 gacgtcgaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
     5401 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
     5461 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
     5521 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
     5581 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt
     5641 tgtaactatg cacaaatgtt aaatttaccc atcaaggggt gaccttaaca gaattgaaac
     5701 gaaaaaatat ttcctctgaa ccgattcacc aagcaacaaa acgcataaca attcaattat
     5761 gtgaattaat atgtaaaatg aatgttcagg gaaggagaga aaggtagtat ttacctatgt
     5821 tttcttttta cattgcttat tatcactgca atcgccacca taaccttctc gtagcgaaaa
     5881 tcatcactta accagaacgt gatgattttc aggtgacaga ttctgtttag agttcattct
     5941 gaatctttga acaaaggcaa tccttgcgaa cgccgccatt ctttgacgat ttt
//
LOCUS       DAOFSV010000208         5914 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.208, whole genome shotgun sequence.
ACCESSION   DAOFSV010000208 DAOFSV010000000
VERSION     DAOFSV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.208"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            4..267
                     /locus_tag="QBH27_004403"
     CDS             4..267
                     /locus_tag="QBH27_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDQ7016633.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            368..1009
                     /gene="lipB"
                     /locus_tag="QBH27_004404"
     CDS             368..1009
                     /gene="lipB"
                     /locus_tag="QBH27_004404"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDQ7016634.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            1269..1793
                     /locus_tag="QBH27_004405"
                     /pseudo
     CDS             1269..1793
                     /locus_tag="QBH27_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            2034..2999
                     /gene="lipA"
                     /locus_tag="QBH27_004406"
     CDS             2034..2999
                     /gene="lipA"
                     /locus_tag="QBH27_004406"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDQ7016635.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(3099..3302)
                     /gene="tatE"
                     /locus_tag="QBH27_004407"
     CDS             complement(3099..3302)
                     /gene="tatE"
                     /locus_tag="QBH27_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDQ7016636.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(3431..4219)
                     /locus_tag="QBH27_004408"
     CDS             complement(3431..4219)
                     /locus_tag="QBH27_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDQ7016637.1"
                     /translation="MLVAAGQFAVTSVWEKNVEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            4312..4695
                     /gene="crcB"
                     /locus_tag="QBH27_004409"
     CDS             4312..4695
                     /gene="crcB"
                     /locus_tag="QBH27_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDQ7016638.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(4749..4958)
                     /gene="cspE"
                     /locus_tag="QBH27_004410"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(4749..4958)
                     /gene="cspE"
                     /locus_tag="QBH27_004410"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDQ7016639.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(5133..5693)
                     /gene="pagP"
                     /locus_tag="QBH27_004411"
     CDS             complement(5133..5693)
                     /gene="pagP"
                     /locus_tag="QBH27_004411"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDQ7016640.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1588 a         1475 c         1296 g         1555 t
ORIGIN      
        1 gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt
       61 atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg
      121 ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct
      181 atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa
      241 atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt
      301 aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga
      361 tgccgttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc
      421 aatctcccag gctatgcatg aattcaccga tacccgcgat gaaagtaccc ttgatgaaat
      481 ctggctggtc gagcactatc cggtattcac ccaagggcag gcaggaaagg cggagcacat
      541 tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca
      601 cgggccgggg caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt
      661 gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat
      721 agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc
      781 actgggttta cgtattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat
      841 ggatctttca ccatttttac gtattaatcc ttgtgggtat gcaggaatgg aaatggctaa
      901 aatatcacaa tggaaacccg aagcgacgac taataatatt gcaccacgtt tactggaaaa
      961 tattttagcg ctactaaaca atcctgactt cgaatatatt accgcttaat tccatacatc
     1021 aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt
     1081 cgcgtttgta tattttcttt atcacttgca gaaatgcgat gctacgcctt gctccgtagc
     1141 ccacctaatt gattaactca ttgaaataat tgaccatggt gagcaaaagc gttccgtccc
     1201 aattagacat aagattattg taattttttt aaatattatt ttttcatgaa taaaattgga
     1261 gtaagtgcgt ggatagtaat aataaaattg aaccctgttt aagcagaaaa tcgtcagaag
     1321 gtaaaccaca aatatttaca accttacgaa atattgatct tacccttctg actattttga
     1381 agttgcatat gtccataaag gaatcgttaa tgcagcgaaa gtgcttaatc tgccccccct
     1441 cggcaatcta tcagcctatt cagaaactgc gcgttatatt ccctgaccta ttgtttattc
     1501 gcaaaggcca gggtgtcact cctaccgcat ttgcgatgta tctatatgag tctatcactc
     1561 agggacttga gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgga
     1621 cgataactat tgcgccctcc ccctcggtcg gagccctggt ccttcctgtc atctatcggg
     1681 cgattaaaac tcactatcca cagcttttac tgcgcaaccc gcccatcagc ggcgcggaaa
     1741 accaactcag tcagtttcaa accgatctca tcatcaataa catgttttgc acggggtcat
     1801 agatgttatc cgtaatgcat tttgaaaaag tattgttaca gccagatcac agcgcacaac
     1861 gataaaatgg cacaatccaa caacaatttt acattttcgc gaccgctttg gctgctttca
     1921 ggtccgtttc aatgatatac tgccagtcgt taattcaaaa atagttgata attacaacaa
     1981 tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg gaacacgcac gctatgagta
     2041 aacccattgt gatggaacgc ggtgttaaat accgcgatgc cgataagatg gcccttatcc
     2101 cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa
     2161 tcaagcttcc agcggactct acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg
     2221 gcctgcattc tgtctgcgag gaagcctcct gccctaacct ggcggaatgc tttaaccacg
     2281 gcactgcaac gtttatgatc ctcggcgcta tttgtacccg ccgttgcccg ttctgtgacg
     2341 ttgcccacgg tcgcccggta gctcctgatg ccaatgaacc agtgaaactg gcgcagacca
     2401 ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt tgaccgtgat gatctgcgcg
     2461 atggcggtgc ccagcacttt gcggattgca ttactgccat tcgggaaaaa agcccgcaaa
     2521 tcaaaattga aactctggtg ccggatttcc gcggtcgtat ggatcgtgct ctggatattc
     2581 tgactgcaac gccaccagat gtgttcaacc ataacctgga aaacgtaccg cgtatttacc
     2641 gtcaggtacg gcctggtgca gattacaact ggtcgcttaa gctgctggaa cgctttaaag
     2701 aagcgcatcc ggaaatcccg accaagtctg gtctgatggt gggactgggt gaaaccaatg
     2761 aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg tgtgacgatg ctaacgctgg
     2821 ggcaatattt gcagccaagc cgccatcacc tgccggttca acgttacgtt agcccggatg
     2881 agttcgacga aatgaaagcc gaagcgctgg cgatgggctt tacccatgct gcatgcggtc
     2941 cgtttgtccg ctcttcttac cacgccgatt tgcaggcgaa agggatggaa gttaagtaat
     3001 cctgtaataa ttacttacat ctgtctgata aaaaacccgc tttttgaagc gggtttttgt
     3061 atcgaacaag atattgaggg agcgtcctgc tcgccacgtc actctttatg agagagcttt
     3121 tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc
     3181 ttcttgaacc ctttaatggc cgctccaagg tctccgccca gcgtacgtaa cttcttagtc
     3241 ccaaacagca gaacgaccag cgccgcaact accagcagtt tggtaatact aatctcaccc
     3301 atagatacct tcttgacata taaccggcaa cagaacgcgc cttaaaaacc agagaatatt
     3361 aacggtcatt cgacgcgcgc acacaatagc aatctgtaac aaggtgaatc aagcgccgag
     3421 taaaaaaaca tcataataat tgcggcggcg caaagcgacg gttgtttaag acgggcagtt
     3481 gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc cataatgagt gcaggcattt
     3541 ctgacgctgc cgcaatggtg acgccaaagg gatctataat ccggctttgc ccgatatttt
     3601 tgttcccgca ctcccccgcc gccaccatat aacaggtggt atccagcgca cgagcggcaa
     3661 gcaacgttga ccagtgatgc tctttgagcg gcccacgaac ccaggcggca ggaagtacca
     3721 gtatttcagc tccctgtaat gcctgtgcca gcgccagctc tggaaagcgt aagtcataac
     3781 aggtcatcag accgaccttc atcccctcca cctccagtaa cggagctatt tcattaccag
     3841 catcaacatg gcgtgattcc tgaatggcaa atgcatcata gagatgcagt ttggcataac
     3901 gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt ccatgcgcgc cccggtgttg
     3961 aaggaacatg aatcgtcaga attgtcgtca tcatgttacg tttactttct cgccgtaaac
     4021 gaccgaggaa ttcgccttcc agcagttgtg ctgatttaac cgatagatct gcatcatgat
     4081 catcacgcgc cagtaatgct tccggcagga caaacagcga aacctcgttt tccgccgcct
     4141 gcgccatcaa cgaggcacaa atctcaacgt tcttttccca cacagatgta acagcaaact
     4201 gtcctgcggc aaccagcatt gccacctcct gtcagagtcg taaaattcgc caacggcgat
     4261 tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc agtgttacaa
     4321 cttcttttag cagtttttat tggcggtggt acgggaagcg tggcgagatg gctgttaagt
     4381 atgcgattta acccgctgca tcaggcgatt ccgttgggaa cgctggcagc aaacctgatt
     4441 ggggcattca tcataggaat gggattcgcc tggttcagca ggatgacgaa cattgatcca
     4501 gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt ctcaacattt
     4561 tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcgtt actgaacgtt
     4621 ttcgtcaacc ttttggggtc ttttgccatg accgcactgg ctttctggct gttttccgcc
     4681 tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg aattcttgct
     4741 gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc gttagtgatt
     4801 tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg gattgcagag
     4861 aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa tcctttggac
     4921 tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt tacatgaaaa
     4981 atagacacaa atgctgtgtc ggttaccagt acaccaattg tggtacgctt tgtccagtgc
     5041 aactttacta aaaagtgata aaagtcgcgt tatttttgat gttaattatt ttagtggttg
     5101 attttaaaga atttactaaa acttcatttg tctcaaaact gaaagcgcat ccaggcaaag
     5161 tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaacgt cgcggggcca
     5221 taccccacag aggccaatgg cagtagaacc ggaagaggga tgtaattcca gttatcgcgc
     5281 gccgttacgc cagcggtaaa tcccaggcct aaatgaaaat tttcatccgc cagcggtcgc
     5341 caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga gtccttaaat
     5401 gccattgcat acaggccatg ccagtttcct ttttcatccc aacgcgactg accaaaaccg
     5461 ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcataggc aaaacgcgca
     5521 tgccaggtga tggcaggaat atataaatca taatgttctg gctgttgcca ggtttgtgca
     5581 atattttctc taaacgttgt catccactca tcagcgttag caaaaacttt accaacactg
     5641 attaactgaa taaaaacaaa gaaaaatata gcgacatatt tactcacgtt catttgtgac
     5701 cattaaacat ttatcaaaaa tctactacta acatagcaaa actacaatta acataacctc
     5761 aatagcccca acatgaagaa taatatgaat agctgctcta tttatttccg ctagttcaag
     5821 ataaatattt ccgcgcatcg attaaagacg atgcatcgtt ttctgcgatg ggaatagtca
     5881 taaaagaaaa accaagtcgt ttctgataac aaaa
//
LOCUS       DAOFSV010000209         5911 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.209, whole genome shotgun sequence.
ACCESSION   DAOFSV010000209 DAOFSV010000000
VERSION     DAOFSV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.209"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            227..766
                     /locus_tag="QBH27_004412"
     CDS             227..766
                     /locus_tag="QBH27_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDQ7016641.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            829..1062
                     /locus_tag="QBH27_004413"
     CDS             829..1062
                     /locus_tag="QBH27_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDQ7016642.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1128..3086
                     /locus_tag="QBH27_004414"
     CDS             1128..3086
                     /locus_tag="QBH27_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDQ7016643.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NCWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3141..3575
                     /gene="psiB"
                     /locus_tag="QBH27_004415"
     CDS             3141..3575
                     /gene="psiB"
                     /locus_tag="QBH27_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDQ7016644.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3572..4291
                     /locus_tag="QBH27_004416"
     CDS             3572..4291
                     /locus_tag="QBH27_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDQ7016645.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            4537..5906
                     /locus_tag="QBH27_004417"
     CDS             join(4537..5017,5017..5906)
                     /locus_tag="QBH27_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HDQ7016646.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
BASE COUNT         1456 a         1447 c         1773 g         1235 t
ORIGIN      
        1 atcccgcaaa actttttgcc cagaagcagg caaaaacttt tgcggtctgc ccccggacag
       61 tcgtttccgt ccgtggcagt gatgccccat gcggcgaagg ttctcaaaaa ggtgatggtg
      121 aataccggag ggaacaaaag gcaggtcccc gcaaaacgtc aaaaccgcgg ctcccgaaac
      181 tcaaaagatg atgggggctg aaaacatcaa cagaaggagg cacatcatgg cagttcgtgg
      241 cattaacaag gtcattctcg taggacgcct gggaaaagat ccggaagtcc gttacatccc
      301 caacgggggc gcagtggcaa acctgcaggt cgccacgtcg gaaacctggc gtgacaaaca
      361 gacaggggag atgcgggagc agacagaatg gcatcgcgtg gtgctgttcg gcaagctcgc
      421 ggaagtggct ggcgaatatc tgcgcaaggg cgcgcaggtc tacatcgaag gtcagcttcg
      481 cacccgtagc tgggaagata acggtatcac ccgttacgtc accgaaattc ttgttaagac
      541 cacaggcacc atgcagatgc tgggacgtgc cgcaggtgct cagactcagc cggaagaggg
      601 gcaacagttc agcggtcagc ctcagccgga accacagtcg gaggccggta cgaaaaaagg
      661 tggcgcaaaa acgaaaggcc gtggacgtaa ggccgcgcag cccgagcctc agccgcaacc
      721 gccggagggg gaggattacg ggttttcaga cgatatcccg ttctgatcgg gctgactgtg
      781 ataaccgccc cgccccgttc tgtgcggggc atcaccggag agatgagaat gagcgaatat
      841 ttcagaatac ttcaggggct gcctgacggt ccttttaccc gcaaacatgc cgaagctgtt
      901 gccgcacagt accggaacgt ctttatcgag gatgaccacg gcgaacagtt tcgcctggtt
      961 gtccgtaata acggtgcaat ggtctggcgt acctggaatt ttgaggacgg tgccgggtac
     1021 tggatgaacc atgtcatcag ggatttcggg attcttaagt aagagaggtg ccggacgggg
     1081 acacgtccgg cagacaatca ttattaccgt tttgggaggt actaactatg tcagttacag
     1141 agtctaaggc aaaaacggag cgtaaatcca gccgtaaacc tgcaaaaacg caggaaacag
     1201 tcctgtctgc cctgctggcg cagacggagg aagtgagcgt gccgctggcc tcactgatta
     1261 aatcaccgct gaatgtgcgc acggtgccgt attctgtgga gtccgtcagc gaactggctg
     1321 agtccattaa gggcgttggc ctgttgcaga atctggtcgt gcataccctg cctggtgacc
     1381 gttacggtgt cgccgcaggt ggtcgccgac tggctgcact caacatgctg gcagagcgca
     1441 acatccttcc ggctgactgg cctgtacgcg tgaaggttat tctgcaggaa ctggcgactg
     1501 ccgcatcaat gactgaaaac ggtcatcgtc gggatatgca ccctgccgaa cagattgccg
     1561 gattccgcgc aatggcgcag gaaggcaaaa cacctgcgca gactggcgac ctgctgggct
     1621 attcaccccg ccacgtccag cgaatgctga aactggcaga ccttgcaccc gtcatccttg
     1681 atgcgctggc agaagaccgc atcaccaccg aacactgtca ggcgctggcg ctggagaacg
     1741 acaccgcgcg tcaggtgcag gtgtttgaag ccgcctgcca gtcaggatgg ggcggtaaac
     1801 cggatgtgcg ggttatccgc aacctgatta ccgaaagtga agtggcggtg gctgggaaca
     1861 gtaaattccg cttcgtgggg gctgatgcct tctcgccaga cgaactgcgc accgatttgt
     1921 tcagcgacga cgagggtggc tatgtggact gcgtggcgct cgatgccgcc ctgctggaaa
     1981 aactccaggc tgtcgcagag ttccttcggg aagccgaagg ctggggatgg tgtgccggac
     2041 gcatggagcc tgtaggtgag tgccgtgagg atgccggaac ataccgctgt ctgccggagc
     2101 cggaagcggt gctgacggag gcggaagaag aacgtctgaa cgaactgatg acgcgttacg
     2161 acgcgctgga aaaccagtgt gaggaatctg acctgctgga agcagaaatg aagctgatgc
     2221 gctgcatggc gaaggtcaga gcgtggacgc cggagatgcg tgccggaggt ggagtggtgg
     2281 tgtcctggcg ttatggcaac gtgtgtgtcc agcgaggtgt gcagttgcgc agtgaggatg
     2341 acgtggctga cgacgctgac cgcacggaac aggtgcagga gaaagcgtca gtggggggaa
     2401 tcagtctgcc actgctgacg aaaatgtctt ccgaacgcac gctggcagtc caggcggcgc
     2461 ttatgcagca accggacaaa tctctggcac tgctggcatg gacgctctgc ctgaatgtgt
     2521 ttggcagcgg agcgtacagt aaaccggcac aaatcagcct ggaatgtaaa cattattcgc
     2581 tgaccagcga tgcgccgtca gggaaggaag gtgtcgcatt catggcgctg atggcagaaa
     2641 aagcccgtct tgctgccctg ttaccggagg gatggtcacg agacatgacg acattcctgt
     2701 cactcagtca ggaggtgctg ttgtccctgc tcagtttctg caccgcatgc agccttaacg
     2761 gtgtccagac ccgtgagtgt ggtcacacgt cacgcagtcc gcttgactcg ctggaaaccg
     2821 ctatcggatt ccacatgcgc gactggtggc agccgacaaa agcaaacttc ttcggacacc
     2881 tgaaaaagcc gcagattatc gcagccctga atgaggcagg actgtccggt gctgcacggg
     2941 atgcagagaa gatgaagaaa ggtgatgcgg ctgaacatgc agagcaccat atgaaagaca
     3001 actgctgggt gcctggctgg atgtgtgcac cacatccaca gacggatacc actgaacgca
     3061 ccgataacct ggctgatgcc gcctgataaa caaccacacc gccccgccgg agacggggcg
     3121 gcagcaaggg agataccgtg atgaaaactg aactgaccct gaatgtatta cagaccatga
     3181 gcgcacagga atatgaagat atccgggcag ccggaagtga tgaacgtcgt gagctgacac
     3241 acgccgtgat gcgggaactg gatgcgccgg acaactggat gatgaatggc gagtatggca
     3301 gtgagttcgg gggctttttc cccgtccagg ttcgtttcac gccagcccac gaacgtttcc
     3361 acctggcgtt atgttcaccg ggcgatgtct ctcaggtctg ggtgctggtt ctggtgaatg
     3421 ccggtggtga gccgtttgcg gtggttcagg tacagcggcg gtttgcaccg gaagccgtca
     3481 gccattcact ggcactggcg gcgtcactgg acgcgcaggg gtacagcgtc agtgacatca
     3541 tccatatcct gatggcggaa ggaggtcagg catgagcgca cgttcacagg cactggttcc
     3601 tctcagcaca gagcaacagg ccgcatggcg ggcggtggcg gagaccgaaa aacgccgcca
     3661 tcagggtaac acgctggctg aatatccgta tgcgggggcg tttttccgct gtctgaacgg
     3721 aagccgcagg atatcgctgt ctgacctgcg atttttcatg ccgtccctga ccgcagaaga
     3781 actgcacggc aaccgtcttc agtggctgta tgccatcgat gtgctgattg aaacacaggg
     3841 cgaggtctgc ctccttcccc ttcccggtga tgcagcagag cgcttgttcc cgtcggtgcg
     3901 gtttcgtgtc agggaacgca gtcgccataa atccgcgctg gtcatgcaga agtacagccg
     3961 ccagcaggcg agggaggcag agcagaaagc ccgtgcgtac caggcgctgg tcgctcaggc
     4021 tgaaatcgaa ctggcattcc attcgccgga aacagtcgga agctggcatg cacgctggag
     4081 tgaccgggtg gcagagcatg acctggaaac cctgttctgg cagtggggcg agcgttttcc
     4141 gtcactggcc ggaatggaac gctggcagtg gcaggatatg ccattctggc aggtcatcgc
     4201 agaggctggt atggcggcaa gggtggtcgg tcatgctgtt cgtgagatgg agcggtggat
     4261 ggtgccgaac aaactccggg aggcagcgtg atgcggcaac aatcacacgg atttcccgtg
     4321 aacggtctga atgagcggat tattttcagg gaaagtgagt gtggtcagcg tgcaggtata
     4381 tgggctatga tgtgcccggc gcttgaggct ttctgcctca tgacgtgaag gtggtttgtt
     4441 gccgtgttgt gtggcagaaa gaagatagcc ccgtagtaag ttaattttct gtactgcacc
     4501 cattttgttg gacgatgaaa tggaatagcc cctaatatgt caaagccaaa ataccctttt
     4561 gaaaagcgcc ttgaagtcgt gaatcactac ttcacaactg atgatggtta caggatcatc
     4621 tcggcacgtt ttggtgtccc ccgaacccag gtcaggacat gggttgccct ctatgaaaaa
     4681 catggagaaa aaggtttaat tcccaaacct aaaggcgtta gtgctgatcc agagttgcgt
     4741 attaaggtcg tgaaagctgt gatcgagcag cacatgtccc ttaatcaggc tgctgctcac
     4801 tttatgcttg ctggtagtgg ttctgtagcc aggtggctga aggtctatga agagcgcgga
     4861 gaagctggtt tacgcgcgct caagattggc accaaaagaa acattgcaat atcagttgat
     4921 ccagaaaaag cggcatcagc attggagctg tcaaaagacc gacgcattga ggatcttgaa
     4981 aggcaagttc gatttcttga aacgcggctt atgtatctaa aaaagctgaa agccttagct
     5041 catcccacga aaaagtgaaa gtactcaacg agctaaggca gttttatcct cttgatgagc
     5101 ttctcagggc tgcggagata ccgcgcagta cgttttatta tcatctaaag gctctcagca
     5161 agcctgacaa gtatgcggac gttaaaaagc gtattagtga gatttatcac gagaatagag
     5221 gccgatacgg ataccgtagg gtaacgctgt ctcttcatcg agaaggaaaa cagattaacc
     5281 ataaagctgt tcagcgcctg atgggaaccc tctcacttaa agcagcgatt aaggtcaagc
     5341 gataccgctc ttacagagga gaggtagggc aaaccgcccc taatgttctc caaagagatt
     5401 tcaaggctac gcggccaaac gagaagtggg ttaccgatgt tactgaattt gcagtcaatg
     5461 ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt atttcttaca
     5521 gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgatcag gcattcaaaa
     5581 agcttaatcc tcacgagcat cctgttctgc actctgacca gggatggcag tatcgtatga
     5641 gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc agaaaaggca
     5701 attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg gagtgttttt
     5761 atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa tatattgaat
     5821 actacaacag cagaagaatt agcctgaaat taaaaggtct gactccaatt gaatatcgga
     5881 atcagaccta tatgcctcgt gtttaactgt c
//
LOCUS       DAOFSV010000210         5869 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.210, whole genome shotgun sequence.
ACCESSION   DAOFSV010000210 DAOFSV010000000
VERSION     DAOFSV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.210"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(187..690)
                     /gene="cheW"
                     /locus_tag="QBH27_004418"
     CDS             complement(187..690)
                     /gene="cheW"
                     /locus_tag="QBH27_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDQ7016647.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(711..2675)
                     /gene="cheA"
                     /locus_tag="QBH27_004419"
     CDS             complement(711..2675)
                     /gene="cheA"
                     /locus_tag="QBH27_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDQ7016648.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTSPMLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(2680..3606)
                     /gene="motB"
                     /locus_tag="QBH27_004420"
     CDS             complement(2680..3606)
                     /gene="motB"
                     /locus_tag="QBH27_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDQ7016649.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKRPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3603..4490)
                     /gene="motA"
                     /locus_tag="QBH27_004421"
     CDS             complement(3603..4490)
                     /gene="motA"
                     /locus_tag="QBH27_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDQ7016650.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4617..5195)
                     /gene="flhC"
                     /locus_tag="QBH27_004422"
     CDS             complement(4617..5195)
                     /gene="flhC"
                     /locus_tag="QBH27_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDQ7016651.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5198..5548)
                     /gene="flhD"
                     /locus_tag="QBH27_004423"
     CDS             complement(5198..5548)
                     /gene="flhD"
                     /locus_tag="QBH27_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDQ7016652.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         1399 a         1576 c         1414 g         1480 t
ORIGIN      
        1 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
       61 caagttgatc tcgttatcgg caaggagggg ggaaacttta ttgctgatgc cacccgccgc
      121 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggacatcg
      181 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      241 cagtttttcg atgttcacca gaatcaacat gcggtcgccc agtgcgccca gtcctgtgag
      301 atattcggtt gaaagcgtca cggcaaattc cggtgccgga cgaatttgct ccgccgtcaa
      361 tgaaagcacg tctgagacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      421 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      481 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      541 gttcgcaatc cgtgtcacct gatcgtagcc acggatctcc tgcactttca gaatatcaat
      601 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
      661 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
      721 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
      781 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
      841 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac tagcaaggcg
      901 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
      961 cccgcgacgt tgaacacttt ccacagttcg acgataggca gatattcacc ccgcacttcc
     1021 agcacccgct cgccgccagc cagtggatgc agatcggctt cacggggttg cagtgattcc
     1081 atgacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtca
     1141 agaattgcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcatcgac
     1201 tggatctcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1261 ccacgcccgg agacgtcggt gacctgctcc gccgtggaga agccaggtgc aaatatcagc
     1321 attgcaactt cgtcgtcgct catgttttcg ctgacggtca gaccttgcga ggccgctttt
     1381 gccagaattc gttcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1441 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcagcgagc
     1501 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1561 tcgataatgc gttcaatcag gctcttgtcg agttcagtag aactgcccac cagcgtcagt
     1621 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     1681 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     1741 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     1801 gaacgctggg caagcatgga ctgggtgatg accagctcac cgacgaggtt aattaattga
     1861 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     1921 cgctccacgc gaccggttgg cgcttgttcg gctgccagtt taagcattgg tgaggtggat
     1981 atttttggcg agacgtctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2041 agtaccgctg tgatgtcatc ttcggcgatg tcgcccggta atattgccga gagggaatca
     2101 gcccctttca ccacgtcagt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2161 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gtgactgact acgactctgc
     2221 tcatcttgtg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2281 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgaa gctggcggca
     2341 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2401 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2461 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaggt tcctgcccct
     2521 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2581 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     2641 aatgtctgat aaaaatcgct tatatccatg ctcacgctgt cacctcggtt cggctgatgg
     2701 cattgtggga acactgacct gtggtgcaac ctcaggtttt tccagggcgc ttactggctc
     2761 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccag
     2821 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     2881 cgccatgccg acgacacgta acactttgcc gccattcaac ccgccaacca tcagttcgcg
     2941 gcgggatgca ttggcacgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3001 ggcgtaggga aaatcatcgg tatgacccga gaggctaatt cggttgggaa taccgttcag
     3061 tacgggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacgtcgg cactgccagt
     3121 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3181 atcgattttg agatgtggac gtaacgcccg cagtttcgga tcggactcaa tcaactggtc
     3241 gagatcgcca cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3301 gttcggccgc ttattcactt ccccctggct ttgggtgtaa tcatcaccac cgccgggaat
     3361 tgggctttca ctattagaaa tgcgatcgcc gcccgtaacc gctgtcgcca gcggagtccg
     3421 gaagtactcc gcaatctgaa tcagctcttt tggactggag atagagatca gccacatcac
     3481 cagaaaaaag gccatcattg cggtcataaa gtcggcataa gcaatcttcc acgatccatg
     3541 tgctgccccg tggcttttgg ctttacgtcg tttgacgaca ataatcggat gcgcttgatt
     3601 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     3661 tccagctcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     3721 gcgataggcg gtgcgtaacc gttcagattt gaaagcagag tgactttgac gcactgcatc
     3781 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctaatgggga aataaatccg
     3841 taagccaata aaataccgag gaaagtcccc accatcgcat gagcgataag cgcccccagc
     3901 tcggcggcag gacgatcggc tgaacctaac gcgtgaacta cccccattac agccgcaaca
     3961 ataccaaaag ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4021 tcgctttcgt gcgtctcaat ttcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4081 tgaccgctga taatcaggcg cagataatcg acgataaatt caagcatgac gctatccgca
     4141 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcgcgttcc
     4201 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4261 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgccttcagc
     4321 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4381 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4441 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4501 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacacgcg ataggggcaa
     4561 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttt cgccacaccg tatcagttaa
     4621 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     4681 agtttacgtc tttttactgc ccgggatggc ggctgacata agctgcaggc aaagctgcca
     4741 acaggctggt gagcatgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     4801 agtaatccac tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc cagcagcggt
     4861 ccttcttctg cttgcgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     4921 acgccattac acaagccagt tttcagtaaa aactgccatg cattacagaa catcgaagca
     4981 tgaacatttt gttcccaggt cataaaccag tcggttgaaa atggcagcat gcctttcggc
     5041 ggtgggcttc cgcgcagttc tttatagagt tttatcagac gtccgcgact taactgtgtt
     5101 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5161 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5221 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5281 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5341 caaaacggaa gtgacaaacc agttgattgg tttctgccag cttaaccatt tgcggaagag
     5401 tcagtgccgc taacgttgtc gccatttctt catttatgcc gagacgaaac atagcggacg
     5461 ctttgtcctg aacaatcaga cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa
     5521 tgtgtttcag caactcggag gtatgcatta ttcccaccca gaataaccaa ctttattttt
     5581 atgcggtctc accgcacccc gtgatgtcgc cgggaagccc cggtaaaaaa taattagcat
     5641 tagaatagtt gcgataagct gcaataagca gacccacctt tttggtttaa taagtcctta
     5701 caaatagaag ggggtcttta cacttatcta agatttttcc taaatcgacg caactgtact
     5761 cgtcactaca cgcacataca acggaggggg gctgcgattt tcaataatgt gtgatgcaga
     5821 tcacacaaaa cgctcaatta cttaacataa atgtataagt catactttt
//
LOCUS       DAOFSV010000211         5782 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.211, whole genome shotgun sequence.
ACCESSION   DAOFSV010000211 DAOFSV010000000
VERSION     DAOFSV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.211"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1569)
                     /locus_tag="QBH27_004424"
     CDS             complement(<1..1569)
                     /locus_tag="QBH27_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HDQ7016653.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPFYLWQVCDNGDYQS
                     GRVLQSVGCLLPEGCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GITHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGITGDNAR
                     GRAVGFPWLRTALMSAVCVLAIGGAGMTTSFFANRALVQETSIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGPERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQSVLPSL"
     gene            complement(1626..2840)
                     /locus_tag="QBH27_004425"
     CDS             complement(1626..2840)
                     /locus_tag="QBH27_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016654.1"
                     /translation="MPVRLNTIPDAEPYPVPPARIKWLTALLLMLSVGVALTSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVASVRWLFFITQYIRTDAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETITAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEVFSRLFSDIRLQLTRLPDDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNHIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPECITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            complement(2918..3367)
                     /locus_tag="QBH27_004426"
     CDS             complement(2918..3367)
                     /locus_tag="QBH27_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016655.1"
                     /translation="MQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKSYDFFGDVVLSQ
                     CINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILI
                     IIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYIIFALNK"
     gene            complement(3470..4072)
                     /locus_tag="QBH27_004427"
     CDS             complement(3470..4072)
                     /locus_tag="QBH27_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDQ7016656.1"
                     /translation="MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAI
                     RLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFAGKNKHNAKGLMQMQRNAVRQVF
                     KYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIE
                     EAYRTYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR"
     gene            complement(4094..>5782)
                     /gene="vgrG"
                     /locus_tag="QBH27_004428"
     CDS             complement(4094..>5782)
                     /gene="vgrG"
                     /locus_tag="QBH27_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ7016657.1"
                     /translation="ADNFLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLM
                     PGLEIKVQGDDAPAVFRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCP
                     VMAGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDT
                     YGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKI
                     RLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFI
                     SSDAQDKAQGKVREMAPAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVK
                     DLTQAAILLSAPKGVAIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVF
                     VRKAGIKLFANKGAISVQAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYI
                     RLDACGIEAGTPGEYNVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQ
                     PLINWPYELELESGLKMSGITDENGNTELISSDKEEVVNISVFEPDEFLDDEIN"
BASE COUNT         1256 a         1582 c         1450 g         1494 t
ORIGIN      
        1 cagtgacggc aacacgctct gccgcacgcc ttccgggata ttttcatagc gcaggccgtc
       61 tgccatcagc gtggtactga aaaaggcagc gtcggccttt tccgggcggg aagtcatcag
      121 caatgcctta agctgcttat agcgttgttc accggtggcg gtacgctgag gactgttggg
      181 cggcagggcg acaaaggcgt tcagttgctg ttgcagatgg tcaacggcca cgtcgcgcac
      241 cagccggttt gccgcctgcg catagccggg aaaggcggcg gcgagcagtt gctggttacg
      301 ttcagggcca aaacgctgat accacggcgc accttcgcgg atacggtatt gcaggcgttc
      361 cagttcgccc tgcagggtat gcagcgccac cagttgttct gccagcggca ggcgggtatc
      421 gagcgcccgc gccgtctgga tgctggtttc ctgaacaagt gcgcggttgg cgaagaacga
      481 ggtcgtcatt ccggcccccc ctatcgccag cacgcagaca gcggacatca gtgcggtacg
      541 cagccacgga aagcccaccg cgcggccccg cgcgttatcg ccggtgatgc ctgcccagac
      601 cggcgacggc agccacgcat gaggtgcggc ctccggtacg gcggcaagcg gcgggctgaa
      661 catcaggccg cgcaggcgca gggaagaaaa tgcgccgcct gccagcagcg gtttcaggac
      721 ggtctgccag tgggtgatac cccgctctgc aagggtatgg gccagacgca gcagaaaatc
      781 atggcggtta tcggtcagca gctgcgacat gccctgttcc gtcagcggta gggtctgtgc
      841 ttccagcatt gcagccagtt gttccggggt acagccttcc ggcagcaggc agccgacgct
      901 ttgcaggacg cgcccggact gataatcacc gttgtcacag acctgccaca aatagaatgg
      961 ggcctgaaaa ccgagtcgct taccgccgtt tatcagttcg cgccagcctt tgtcgagttg
     1021 cgctgtctgg cggctctgtt cttctgtcag cggccagatg atgccgtcca gcggacggct
     1081 gcggcgcagt ttttttaagg cggtcagtag cgcgacatca ggctccgctg atggcctgcc
     1141 gccgtatatc agcaccgtgt ggtcgccctc cagccagtgt tgcccggtca gccccggcgc
     1201 gatggcctct gcctgttcag gctcgccggt caccagcagc aggcggattt tacgccgcca
     1261 gaatgggccg tagcggtcgc gcaggaaggc ggcgagttcc accattgccg gatgacggct
     1321 gtcggctttc tttgtcgtca ccgcacgata gtcatctccg gcggcaagct gtctgcggag
     1381 ttcaaagttc tgcacggcag cgtggcggtt cagcagatga taaaccccca tgataatcag
     1441 aaagaccacg cctgcggcaa tcacgccagc catccagtac atttgctgtg agctacccgg
     1501 ttcaacgccc agcaatgccg gtttacggtg aataaacgtg caaagcgcaa ccacaacagc
     1561 cacggtcatg acgatggcag gccctgaaac cgaacggggc cggagacggt ttaatcccgg
     1621 aaccactatg atgcctcctg aggggaaacg aatggcgaca ccacggtcgc ccatgaataa
     1681 tgcccgccct gaacgccgct gagcgtcagt tgggggtgtt gtgattgtat tgccgccagc
     1741 gttgccacag cgacggacag ccacggcgca gccggtccgg agcgccccag aaaggcatcc
     1801 agctcgtaca tcagggcttc ctgagcaaac ggctggttca ggcttaacaa cgcggcacgc
     1861 tgttccctgt ccagttcggc agtccacata ccggatatgg catctggctg caccggcatc
     1921 cagtccagcg cctgtgcaat gcctgacgcc atcatctctg tatcagtgat acattcagga
     1981 cgatgcagca gggccagcgg cgtcagcgcg gtttgcgtca accggtttgc cagcaacagc
     2041 gcggtggcgg attctgccgt ccgttccggg ttcttcggtt ccagacgcac ggaaatcacc
     2101 agcagtacgg ccttttcctg aatgtggttg tccagccagt cgtccacggc ggccagcccc
     2161 ggctccgggg ctgaaaacag acgggcttcc gggagtccgg ctttttgcca ttcgtcctgc
     2221 cagatagttc tcacggaggc ccctgaaagc ggcgcatcaa tttccagtag tacgttgata
     2281 tgaaaatcat cgggtagccg cgtcagttgc agcctgatat cgctgaacag acgggaaaaa
     2341 acttcggata gacgtccggt aaccggttta ttggttaata cagggatgac gctgcgacgc
     2401 acagtatctt ctccgcgcaa gtcagcgtag gtattcaaca cctgctgacg tgccagaaat
     2461 gctgttatag tttctgcaac ggaatcacca ttcagcgcgg tttgtacgga aaaactgagt
     2521 atctgcaacg cccgtcgtcc ccggcgcgtt tcctgcaaaa tcacctcttc ccggcgctta
     2581 ttccaggcat cagtccgaat gtactgcgtg ataaaaaaca gccagcgaac tgaagcaacc
     2641 agtgaccaga taaaggcagg cacaccacag gccagccccc agaaatacgg gccgtttttt
     2701 gccagttcat cgctggcgaa taatgaagtc agggccacac cgacagacag catcagtaat
     2761 aaagctgtca gccacttaat tcgggctggc ggtacgggat agggttctgc gtcgggaatg
     2821 gtattaagtc ggactggcat cagtacatcc ctttattttg cattatcgac cagaagttaa
     2881 taaccaagtg aaatttaaag ttatgtatag agtcatttta tttatttaat gcaaaaatta
     2941 tgtaaccatc ttttgatggt agattatctg atgagtatgc tctttctcct gactttttcg
     3001 cattatcata gataatatat ttccctttcc atgtcccaac atccatttgg tttcggatga
     3061 taattaaaat attgtttttt ccaaaccata ccgcttcggg aatatttctc tctgcgaaac
     3121 aaaagccttc aggagagttc ttacctttaa acccattttc ccaaccagtt accagacaac
     3181 cttttatata tggagaacca tattccagcg caaagataag agaaccattt atgcactgag
     3241 aaagaaccac atctccaaaa aaatcataac ttttagttgg agtatctaaa aaggccccgg
     3301 tgataacatt tctgaatttt agatataatg tatcaccgtc aatggttcta agggaaagca
     3361 actgcacact ttcatttttt tcaattgaca ggttttcacg attttaatct tatcgcctga
     3421 aaaacagggg aaagaaaaaa aacaaagtaa tattaacata atgcgcatac tatcttccta
     3481 ttccctccat tagtatttgt atgttatcag gatctttagc cagtttttca gcgcaaggtt
     3541 ttataacaga atagtatgct tcatctgttc cacgatatgt ccgatatgcc tcctcaattg
     3601 ttgcttcttt atctatccag tattgcaaat actctgtgcc aatcttaata ttttcttttt
     3661 catcaaatat tttatcagat ttataaaatg tatcagcatt agcgaaagct tcatttgtct
     3721 ctttatcagt agtcattctt ccttttattt tttgttgtcg atatttaaaa acctgcctca
     3781 ctgcatttcg ttgcatttgc attaagcctt tcgcattatg tttattctta cctgcaaatt
     3841 gcttaaatct agattcttta tagatttgac aaataatata atcctctccc atgatggatt
     3901 tattattatt acggacaagt tgacgaattt gtttcaatct tatagctgga tctacattca
     3961 cttgttttgg tgcggtgtct tttgctggag ttaatttttc gctcttcttc gttagtgttt
     4021 tttttgctga tttatttgtg gtttcttttt tcttttcgga tttatctgcc atgttatatt
     4081 ctcgctatat gtcttaatta atttcatcgt ctaaaaactc atcgggttca aacacactaa
     4141 tattaacgac ctcctcctta tcagaggata ttagttctgt atttccattt tcatcagtaa
     4201 tcccactcat ttttagccca ctttccagtt ctagttcata gggccaatta ataagtggtt
     4261 gcccagtatt ttcatccagc aaaataaagc tctgattaaa atcctcagac ttaatcaccg
     4321 gaaatgccat cagctccggc gtcagcttcg ccttcggctt acgcccgtaa tatcccgcct
     4381 taacgttata ttctcccggc gtaccggcct ctatcccaca ggcatccagc cggatgtagc
     4441 tgccaccgcc gttaagggtg attttcttct tcgcggtgat tttaatctca tcttccgtac
     4501 tggtgatctc tatcgatttc tgcgccaaca gttccatcag atcgttctgt gcctgtaccg
     4561 agatcgcccc tttattggca aacagtttga tacccgcttt gcgtacaaac acgctcagcg
     4621 cctgacccac gccgataaac atattcttca cgacaccgat atcagcgtga tttcctgcgt
     4681 tcgcgatcag gtttttcgac gccgccagtt gcaggtgctc tccgctggcg atcgccacgc
     4741 ctttgggtgc gcttagcagg atcgcagcct gcgtcagatc ttttacactc tgctgcaaca
     4801 gcgctatctg tgatgacaga tctgccggat cggcattcgc cgtctgcgca tcagtggaca
     4861 gcgacttcat ctgtgactgt gcgccatcca gtattgccat cgccggagcc atttcgcgca
     4921 ccttaccctg cgccttgtcc tgcgcgtcgg agctgataaa gatgcctttc tttgcccgta
     4981 ctgtacccca cagatcggta cgcagttcaa agccttcccc gcgctgctgt tttccggcat
     5041 ccaccagatg gccgaggttg agctggctct tgccgccata ctcggtactg accttgatat
     5101 gctctttgcc gcgctcgtca tcgaggcgga ttttgttgtt cgccggggtg cgcagtacgt
     5161 tgcgcttgta gttctgaatg gtcacatggt cggtgtgtgc cgagtcgtgt tttacgcctg
     5221 caatataagg tcggtccggg ttgccttcct caaacgcgat gctgacttcc gttcccgcca
     5281 gcagcggcag atgcaggccg taggtgtctc ccgcatacgg gcgcgactgg cgtacccaca
     5341 ggctttcata acccggtttc cacgtatcgc ggtcaaagtc gaggttgacc cggtagcgcc
     5401 cgtccttgtc gatatgggcg tagatgtcgt tcttcaccgt gctggtgacc cgtgccggga
     5461 gcgtgccggc catcacgggg caggggataa gcgccgggcg gtagccatag cgttctgagt
     5521 acgggatggc ggtaaacgtc agttcatagc tgcggtcacg cgccgcgctg gccgtcacgc
     5581 cggtaatcag tacgcccttg cggaacaccg ctggcgcgtc gtcaccctga acttttatct
     5641 ccagccccgg catcagcagt gaactggtgc tctggccctg tagaatggcc tgttcattca
     5701 aatagcgttc gtggcggata cgggcataaa acgcgccgct ttccgctgcc tctttatcgc
     5761 ctttttgcag gaagttgtca gc
//
LOCUS       DAOFSV010000212         5743 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.212, whole genome shotgun sequence.
ACCESSION   DAOFSV010000212 DAOFSV010000000
VERSION     DAOFSV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.212"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            179..1975
                     /locus_tag="QBH27_004429"
     CDS             179..1975
                     /locus_tag="QBH27_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HDQ7016658.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAIIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVDISYKVGNESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QNTFYDGRDRNINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTIGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1993..2661
                     /locus_tag="QBH27_004430"
     CDS             1993..2661
                     /locus_tag="QBH27_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HDQ7016659.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2676..3347
                     /gene="dsbI"
                     /locus_tag="QBH27_004431"
     CDS             2676..3347
                     /gene="dsbI"
                     /locus_tag="QBH27_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HDQ7016660.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGTLIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3393..4181)
                     /gene="ygiD"
                     /locus_tag="QBH27_004432"
     CDS             complement(3393..4181)
                     /gene="ygiD"
                     /locus_tag="QBH27_004432"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDQ7016661.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4324..5097
                     /gene="zupT"
                     /locus_tag="QBH27_004433"
     CDS             4324..5097
                     /gene="zupT"
                     /locus_tag="QBH27_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDQ7016662.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5159..5329)
                     /locus_tag="QBH27_004434"
     CDS             complement(5159..5329)
                     /locus_tag="QBH27_004434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016663.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1563 a         1370 c         1320 g         1490 t
ORIGIN      
        1 tatccattaa acataaatat ttgtttcacc gaataacaac atatttaaaa gaaacacaaa
       61 aacattctca ctctttattg aattaatatt tatattcacg taacgaattt attagcaccc
      121 tctcaacagt cgatattcat cattattata tatttgcgtt atataaggag atatatccat
      181 gtttgataaa tatagaaaaa cactcgtagc cggaactgtg gcgataaccc tgggtttgtc
      241 agcatcgggg gtgatggctg cgggttttaa accagcaccg cctgccgggc agcttggggc
      301 gattatcgtc gatccctacg gtaatgcgcc cttaaccgcg ctggttgatt tagacagcca
      361 tgttatttct gacgtcaaag ttaccgtcca cgggaagggc gaaaaaggcg ttgacatcag
      421 ctataaagtg ggcaacgagt cactgaaaac ttacgatggt gtaccgattt ttggtcttta
      481 ccagaagttt gcgaacaaag tgaccgttga gtggaaagaa aacggtaagg ccatgaaaga
      541 tgattacgtg gtgcagactt ccgccatcgt caacaattac atggataacc gctccatctc
      601 cgacctacag caaaccaaag tcgttaaagt cgcgccaggt tttgaagatc gcctctatct
      661 ggttaatacc cacaccttta ccgcccaagg ttccgatctc cactggcatg gtgagaaaga
      721 taaaaatgcc ggtatccttg atgcgggtcc ggcaactggc gcactccctt ttgatatcgc
      781 gccattcacc tttatcgtcg atacggaagg cgaataccgc tggtggttgg atcaaaacac
      841 cttctacgat ggtcgtgacc gcaacattaa caaacgtggt tatctgatgg gtatccgtga
      901 aacgccacgc ggcaccttta ccgctgtaca aggtcagcac tggtacgagt tcgacatgat
      961 ggggcaggtg ctcgaagatc acaaactacc gcgcggattt gctgacgcca ctcatgagtc
     1021 cattgagacg ccaaatggca cggtactgtt gcgcgtaggt aagagtaact atcgtcgcga
     1081 tgacggcgta cacgtcacca ccattcgtga ccatatcctc gaagtcgata aatctggtcg
     1141 cgttgtcgat gtatgggatc tgaccaagat cctcgatccg aaacgcgatg cactgctcgg
     1201 cgcgctggat gcaggtgcag tttgcgttaa cgttgacctt gcccatgcag gacaacaggc
     1261 aaaactggaa ccagatacac cgttcggcga cgcgctgggt gtagggccag gccgcaactg
     1321 ggcgcacgtg aactctatcg cctatgacgc aaaagatgac tctatcatcc tctcctctcg
     1381 ccaccagggt gttgtgaaga ttggtcgtga taagcaagtg aaatggatcc ttgcgccctc
     1441 taaaggttgg gaaaaaccgc tggccagcaa gctgctgaaa ccggttgatg ctaacggtaa
     1501 gccaattacc tgtaacgaaa atggcctgtg cgaaaactca gacttcgact ttacctacac
     1561 ccagcatacc gcctggattt ccagcaaagg aacgctcacc atttttgata atggcgatgg
     1621 gcgtcatctg gaacaacctg ccttaccaac catgaaatac tcccgctttg tggaatataa
     1681 gattgatgag aagaaaggca ccgttcagca agtgtgggaa tacggtaaag aacgtggcta
     1741 cgatttctat agcccgatta cctccatcat tgaatatcaa gccgaccgta acaccatgtt
     1801 tggcttcggt ggttctattc atttgttcga tgtcgggcag ccaaccatcg gtaagttgaa
     1861 cgaaatcgat tacaaaacca aagaagtgaa agtggaaatc gacgtgctgt cagataaacc
     1921 caatcagact cactatcgtg cactgttagt ccgtccacaa cagatgttca aataattaag
     1981 aagggaataa aaatgtcgaa attagggatc tcatcactat ttaaaaccat actgcttacc
     2041 gcagctttgg ctgtttcatt taccgcttct gcatttaccg aaggcacgga ttacatggtt
     2101 ctggaaaaac ctattccgaa tgccgacaaa acgctgatta aggtatttag ctacgcctgc
     2161 ccgttctgtt acaagtacga caaagcggtt accggtccgg tatcggaaaa agtgaaagat
     2221 atcgttgctt tcacgccgtt ccatctggaa accaaaggtg aatacggcaa acaagcaagc
     2281 gaagtctttg cggtcttgat caataaagat aaagcagcag gcatttcctt atttgatgct
     2341 aactctcagt ttaagaaagc caagtttgcc tactacgcgg cttatcacga caaaaaagaa
     2401 cgctggtctg acggtaaaga tcccgctgcc tttatcaaaa ctggtctgga tgccgcaggt
     2461 atgagtcagg cggattttga agcggcactg aaagaaccag cagttcaaga aacactggaa
     2521 aaatggaaag cctcttatga tgtcgccaaa atccaggggg ttccagccta tgtcgttaat
     2581 ggtaagtact tgatctacac caagagcatc aaatccatcg acgcgatggc agaccttatc
     2641 cgtgagttag caagcaaata acagggaaac aggctatggg tattaaggga atgtggaaag
     2701 atcttcgcac atcaccagtt gacacactgg tcagatggca ggagcaacgc ttgttgtggt
     2761 tgttaatggc cgttgcgatg ggcacgttaa ttatcctggc gcactctttc ttccagatct
     2821 atctctacat ggcaccctgt gagcaatgtg tatacattcg ctacgccatg tttgtgatgg
     2881 tgattggagg attggttgca gcgatcaatc caaagaacat catcctcaag ctaattggct
     2941 gcgtgatggc attttacggc agcattttgg gactgaagtt ttcgctcaaa ctgaacgata
     3001 tccaccatgc agtacataat ccggatcccg attcactgtt tggcgtacag ggttgctcta
     3061 ccgatcccac ttttcccttt aacctgccgc tggcacagtg ggcaccaaat tggttcaagc
     3121 ctaccgggga ttgtggctat gacgcaccaa tcgttcctga tggcgtcaca ctgagcagca
     3181 cgcaacagtg gtttgtggaa atgtatcagc aatctgaagg ctggtatctg ctaccaccat
     3241 ggcattttat gaatatggct caggcttgta tgctggcttt tggtatgtgt ctggtgctgc
     3301 tggtcattat gagtggcgca tgggcgctta aaatcatcag aggctaactc agcaacaccc
     3361 gaagccagtc attctggctt cgggttcttt tattaaccta tctgcaccga cagcatactt
     3421 aaactgccca tttctatacc atcgaccgga atggtaattg gctcctgccc atcccacgcc
     3481 cctaagacat acaacaacgg cagatagtgc tctggcgttg ggttcgataa cgcgccacct
     3541 tcatggtcga gataattcac cagaggatgt tgttccaccg gcccttgcca cgtcagattc
     3601 gctttcacat actcattaaa cgacatcgcc cacggatacg gtgaactatc accgtgccac
     3661 ttcactgtac gcaggttatg caccacgttg ccactggcga ccaacattat cccttcatca
     3721 cgcagcgccg cgagtttgcg ccccatttcg aaatgccagg cggcaggttt gctactgtcg
     3781 atactcaact gcaccatcgg gatatcggcg tcaggataca tcttaatcag tacgccccac
     3841 gagccgtggt cgaagcccca ggcttcttta tccagcgtca cagggaccgg cgctaacagc
     3901 tcaaccagat gctgtgccag cgcaggtgaa cccggagcag gataatgcgt atcgtacagc
     3961 gcctgcggga agccaccaaa gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct
     4021 gttccacggg taaaccaatg agccgaaacc accacaatcg cttgcgggcg tggcaatgtc
     4081 atccccaact tctgccagct gcgggtatac aaattatctt ccagcacgtt catcggacta
     4141 ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca tgatgatatc cttaactaaa
     4201 ggtgtcattt tgatatcctc acaatacgct tgttcggcgg agtaagaacc cggataacaa
     4261 tgatgatgat catcagttat tttgacgatc tgcctgaagg tgaagattta taaggagttg
     4321 tcgatgtcag tacctctcat tctgaccata ctggcggggg cagccacgtt tattggcgcg
     4381 tttctcggcg ttctcgggca aaaaccctcg aaccgcttac tggcgttttc gctaggattt
     4441 gcggcgggga tcatgttgct catctcatta atggaaatgc ttcctgccgc attagccgcc
     4501 gaaggaatgt cacctgtgtt gggttatgga atgtttatct tcggtctgct tggctatttt
     4561 ggtctggacc gcatgttgcc acatgcgcat ccgcaggatt taatgcaaaa atcggtgcag
     4621 ccgttgccaa aatcgatcaa gcgcacagcc attctgctca ctctcggtat cagtctgcat
     4681 aacttcccgg aagggattgc cacctttgtc acggcgagca gcaacctgga gctgggattt
     4741 ggtatcgccc tggccgtagc gttgcacaat attcctgaag gtctggcggt tgcaggcccg
     4801 gtttacgcgg caacggggtc aaaacgtacc gcaattctgt gggcggggat ttccggactg
     4861 gctgaaattc tcggtggtgt gctggcatgg ttaattcttg gcagcatgat ttctccggtg
     4921 gtcatggcgg caatcatggc ggcggttgcc ggtattatgg tggcgctctc ggttgatgaa
     4981 ttaatgccgc tcgccaaaga gattgacccc aataataacc ccagttatgg cgtgctatgt
     5041 ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa cggcaggaat tggttaaaga
     5101 tgataatgac agatgttgcc cattgctaaa ggcaacatct gtatcgttat taaaggtatt
     5161 attgatgctt ttcaaacact tctttatcgg agcgtttatc taatttactt cgggcagcta
     5221 acagcgcatc tctgtcatgg cgaaaagctt tacagtaacg tttaagatga agaaaatagc
     5281 caccagctag tagaacaatt aatacaatcc agtaccaggc aataaacatc cttttcacct
     5341 tcaaaattta acattactaa acatttagcg ttaaaatttc acatatccaa cgttgaatat
     5401 attcatatac tcgcatagtt gtggatcatg tatatgattt cgatcatagt aagtgttgta
     5461 ttatatctca tgattgttca aaaaacaatc gatcgataat attgattatg tatcaattct
     5521 ccataaaaca ttactcagga taataaaatt taataagtat cactagatat atattatatc
     5581 tttgaagtta ttcctccctc cgattttcat aaaaaacact ccctaatcca cttcaacacc
     5641 cgtgtaaata actgtaaata aatggctatt tatcaatcaa aatatacact aaatatatta
     5701 taaaaacttc aaacagctaa taaccagaaa aatatgctca aac
//
LOCUS       DAOFSV010000213         5685 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.213, whole genome shotgun sequence.
ACCESSION   DAOFSV010000213 DAOFSV010000000
VERSION     DAOFSV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.213"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            133..411
                     /locus_tag="QBH27_004435"
                     /pseudo
     CDS             133..411
                     /locus_tag="QBH27_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(462..707)
                     /locus_tag="QBH27_004436"
                     /pseudo
     CDS             complement(462..707)
                     /locus_tag="QBH27_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1080..1310)
                     /locus_tag="QBH27_004437"
                     /pseudo
     CDS             complement(1080..1310)
                     /locus_tag="QBH27_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1897..2052
                     /locus_tag="QBH27_004438"
                     /pseudo
     CDS             1897..2052
                     /locus_tag="QBH27_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(2049..2495)
                     /locus_tag="QBH27_004439"
                     /pseudo
     CDS             complement(2049..2495)
                     /locus_tag="QBH27_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(2682..2766)
                     /locus_tag="QBH27_004440"
     tRNA            complement(2682..2766)
                     /locus_tag="QBH27_004440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Leu,seq:caa)
     gene            2962..3981
                     /gene="ahr"
                     /locus_tag="QBH27_004441"
     CDS             2962..3981
                     /gene="ahr"
                     /locus_tag="QBH27_004441"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDQ7016664.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVI
                     LKADF"
     gene            4111..5613
                     /locus_tag="QBH27_004442"
     CDS             4111..5613
                     /locus_tag="QBH27_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDQ7016665.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLDIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPMTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASAEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
BASE COUNT         1326 a         1418 c         1553 g         1388 t
ORIGIN      
        1 tgtgatgtct gtcagtctgc tttagggggg actctttcca ttcggtagtt tccttcctcc
       61 ccggcagcga ttttttgaac tttttcttca cccgttgatc ttctcttata tttcattttc
      121 ttcgggtctg ttatggtact ttctgattgt tattcatggg ataacgagca gtttggacat
      181 gcccgtctcg gtgatccgcg tcgtacacga cgccttgtgt cactggcctc ttctctcgct
      241 cagcatgcag gtctttctat tgtgaaatca tcccactcca ctgctcaggt tgaaagtgcg
      301 taccgcctga tacgtaaccc gtctgtgtcc ccggaagcca ttgcctaagc cggatttacc
      361 gccactgcaa gggcatgtga ggctcatccg ctgctcctcg cactggaaga caccaccact
      421 aatcgtaagc gtacagcgag ggccgtattg acggggatgt gttattcagc tggcagtgct
      481 atacgccacg gaagcagttc gctgacccgg ttgaccggcc agtctgctat gacgccaagc
      541 acatggcgaa ggtagctttc tggatccacg tcattcagtt tgcacgtccc gatcaggctg
      601 tacagtagcg ctccccgctc accaccatgg tcagagccga agaacaggaa gtttttacga
      661 cccagactga ccgtcaacgc cacaatcttt gactgtttga cattgtcatg cccgcaggcc
      721 agcgaatcta aagacatcag actgcttgca tcagccagtt gcgtatctgg ctatgtccct
      781 taattggtaa gggttcgagg aactcaacat taacgcattt ttccgcccat caccggcagt
      841 gtatatgaac gattgcactc ttacgatggt tgatttattt ggttgcgtag gggctagcta
      901 gtctggtacc ggaggctgga tctgaagtac tgaccccaag aataacgccg ggcagactca
      961 tattctgacc cattgtcagc ccgcgtgttt actgctctag ctcatttcct gttgtcaggc
     1021 gttcaattcc caactgcagg ttatggcccg gtgcacaaac acctgacggc aggtatcact
     1081 tacccaaaga agataaagcc ctcaagaggc agtaactctg ccaacctctc ctccggccac
     1141 tccgggcaga cgcgttagga cgtccgtcag ccaagcatgt ggctccagac tgctgtttcc
     1201 agaaggctca ttatctgtgc cgctcggttc ccggccatca gtgaaccagc gaacaaccag
     1261 gcctttcgtc ccataacgac cggacggata gcacgctcgc agatattgtt gtgtgtgcct
     1321 tgaaatgcac gatctttccg tcggtgaaag tccgccccga ctaactctcg ctccaactgg
     1381 aaggacagga agcggtccag gaggtaacaa aagggctgaa tctctccctg aaacgacttc
     1441 ttcaggaagt caatgggtta tgcgggccgt aacgtgagtg aacactgagc agccatcgaa
     1501 atgccgtcga tggtgccgat acgccacaat aacggggaag gccgacgatg accgggaaga
     1561 gagtgagaaa ggcatcggtc atacccaccg gggtagtggt gtcagcatgc ataacaattg
     1621 atcgatcgtg caacaagaga agtccataac gatgcctgtc cgggtgacgg gcaacaacgc
     1681 ctcccgaggg ggtgaatcgg ttgttatgga tggcagatgg ggtgtagtag tgaaaatact
     1741 gagtaatgtc ataggagcga aggccccctg ttgcagtacc agtgtaacat gcaggaagag
     1801 cccggggatt gccatcagcc tcacaacccc gctcgaaccg gttcagaacc tacagaaaat
     1861 actacaggag aaagcaaagt gaacctcccc gtgtttcctg tttatcattt tctggtcagt
     1921 gccgcgattc tggtattcgt ggtgattttc tggcggacac accatcgcga ccaccgtaac
     1981 tggctggctc tacggctatt cgtattatgc tctgtaaacc gttggccatt gcggatggtt
     2041 aaaggaacaa taggtgctaa taaatctcga gtttttaact cagcgatatt acgttcacca
     2101 agcgctgcaa aaagattatc ttcaagactt tttagcacac gatgggcgtg atcttcagac
     2161 cactttttat tggtgccatg ccactcaatc gcgacttctt taaaggttcg tgctttactc
     2221 tgttcaacct tatcattttt ctttttgtct cccggatcga cgccattcgc aagcagctta
     2281 cgcgcctcgt cacgacgtac tctggcatcc gctagtgtga tttcaggata aaccccaagt
     2341 gccagcattt tttgctttcc ctcataacgg tactgcaaac gccagtactt agaaccattt
     2401 ggatggacaa gcagatgcat accgccacca tcagtcagct tatattgctt atccgttggc
     2461 ttggctgctc tgactttgat atctgttaat gccatgcgta ttccctccct gttggtacaa
     2521 gcattatcga accggaaata ccatcatctg taccaacata tgttggtaga tgtacgttga
     2581 tatcggttga ccttgagaga gttaatatag cgggaaagat agataaacac tggatttcag
     2641 gcataaaaaa agacctcagt tgaggtctat ttacatactt ttggtgccga aggccggact
     2701 cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg atttcgccac
     2761 ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca cgccaccatg
     2821 caagcgccag cttgctcgca cctcgctaag tgctgaaatt ttcagcattg catacagcga
     2881 tgtgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac ttcccagaca
     2941 acaccagaga aggaccaaaa aatgtcgatg ataaaaagct atgccgcaaa agaagcaggc
     3001 ggcgaactgg aagtttatga gtacgatccc ggtgaactga agccacaaga tgttgaagtg
     3061 caggtggatt actgcgggat ctgccattcc gatctgtcga tgatcgataa cgaatgggga
     3121 ttttcacaat atccgctggt tgccgggcat gaggtgattg gtcgcgtggt ggcgctcggg
     3181 agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg ctggacggcg
     3241 cgtagctgcg ggcattgcga tgcctgtatt agcggtaatc agatcaactg tgagcaaggt
     3301 gcggtgccga cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc ggactggcaa
     3361 tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct gttgtgcggt
     3421 ggtatcacgg tcttcaaacc actgttaatg caccatatca ctgccaccag ccgcgttggg
     3481 gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc aatgggatgc
     3541 gaggtgacag cctttagttc gaatccggcg aaagagcagg aagtactggc gatgggtgcc
     3601 gataaagtgg tgaatagccg cgatccgcag gcactgaaag cactggcggg gcagtttgat
     3661 ctcattatca acaccgtcaa cgtcagcctc gactggcagc cttattttga agcgctgacg
     3721 tacggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt tccggccttt
     3781 acgttaattg cgggcgaccg cagcgtctct ggttctgcta ccggcacgcc ttatgagttg
     3841 cgtaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgactaccga attgttcccg
     3901 atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg ctaccgagtg
     3961 atcctgaaag ccgacttctg atccctttgc cgggtgcctg tcgcccggca cttaaatctc
     4021 tttggaaggc gtctcgcaat ctgctgaatt tagcggtgaa gagtgcgcag ccgttgccta
     4081 tactccaggc aggaaactgg aggaaatctc atgagcgaac ccctgttaat tgcccgcacg
     4141 ccggacacag aactattttt actgccggga atggctaacc gtcacgggct gattactggc
     4201 gcaacgggga cgggtaaaac tgtcacgctg caaaaactgg cggagtctct atcggaaatc
     4261 ggtgttccgg tgtttatggc cgatgtgaaa ggcgatctga ccggcatcgc gcaggcagga
     4321 acggcgtcgg aaaaactgct cgcaaggctt aaaaatatcg gcgtcaatga ctggcaaccg
     4381 catgccaatc cggtggtggt gtgggatatc tttggcgaga aaggccatcc ggtgcgggcg
     4441 acggtttcgg atctggggcc gctgttgttg gcgcggctgt tgaatctcaa cgatgtgcag
     4501 tctggcgtgc tggatatcat cttccgcatt gctgacgatc aggggctgtt actgctcgac
     4561 tttaaagatt tgcgggcgat tacccagtac atcggcgata acgccaaatc tttccagaat
     4621 cagtacggaa atatcagtag cgcatcggtt ggtgccatcc agcgcgggct gttgtcgctg
     4681 gaacagcaag gcgcagcaca cttctttggt gagccgatgc tggatatcaa agactggatg
     4741 cgcaccgatg ccaacggtaa aggcgttatc aatatcctca gcgccgagaa gctttatcag
     4801 atgccgaaac tgtatgccgc cagcctgttg tggatgctct cggagttgta tgaacaattg
     4861 ccggaagcgg gcgatctgga gaaaccaaaa ctggtgtttt tcttcgacga ggcacatctg
     4921 ctgtttaacg atgcaccgca ggtactgctg gataagattg agcaggtgat aaggcttatt
     4981 cgctcaaaag gcgtgggcgt ctggttcgtt tcgcaaaatc cgtccgatat tccggataac
     5041 gtgctcggcc agctcggtaa tcgcgttcaa cacgctttgc ggacttttac gcccaaagat
     5101 cagaaagcgg taaaagctgc ggcgcaaacc atgcgggtca atccggcatt tgataccgaa
     5161 aaggcgattc aggaactggg caccggcgag gcgttgatct cttttctgga tgcgaaagga
     5221 agcccttctg tggtggaacg ggcgatggtg attgcgcctt gttcgcggat gggaccgatg
     5281 acggaagatg agcgtaatgg cttgattaat cactctccgg tgtatggcaa gtacgaggat
     5341 gacgtggacc gggaatccgc ctatgagatg ttgcaaaaag gcgtacaggc aagcgccgag
     5401 cagcaaaata atcctgccgt gaaagggaaa gaggtggcgg tggatgacgg cattcttggt
     5461 ggattgaagg atattttgtt tggcactacc ggaccacgcg gcgggaagaa agatggtgtg
     5521 gtgcaaacaa tggcgaaaag cgccgcccgc caggtgacga atcagattgt acgtgggatg
     5581 ttggggagtt tgctgggggg aagaagaagg taagtcaggc gcgatttgtc tttgcctgat
     5641 gcgacgctgg cgcgtcttat catgcctaca ccactgcaat atatt
//
LOCUS       DAOFSV010000214         5611 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.214, whole genome shotgun sequence.
ACCESSION   DAOFSV010000214 DAOFSV010000000
VERSION     DAOFSV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.214"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..331
                     /locus_tag="QBH27_004443"
     CDS             <1..331
                     /locus_tag="QBH27_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDQ7016666.1"
                     /translation="LLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFRGIS
                     RLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRAR
                     IACRKSP"
     gene            complement(363..1562)
                     /gene="tet(A)"
                     /locus_tag="QBH27_004444"
     CDS             complement(363..1562)
                     /gene="tet(A)"
                     /locus_tag="QBH27_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDQ7016667.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            1668..2294
                     /gene="tetR(A)"
                     /locus_tag="QBH27_004445"
     CDS             1668..2294
                     /gene="tetR(A)"
                     /locus_tag="QBH27_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDQ7016668.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(2326..2562)
                     /locus_tag="QBH27_004446"
     CDS             complement(2326..2562)
                     /locus_tag="QBH27_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDQ7016669.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(2616..3452)
                     /locus_tag="QBH27_004447"
     CDS             complement(2616..3452)
                     /locus_tag="QBH27_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDQ7016670.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3452..4255)
                     /locus_tag="QBH27_004448"
                     /note="aph(3'')-Ib"
     CDS             complement(3452..4255)
                     /locus_tag="QBH27_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDQ7016671.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(4316..5131)
                     /gene="sul2"
                     /locus_tag="QBH27_004449"
     CDS             complement(4316..5131)
                     /gene="sul2"
                     /locus_tag="QBH27_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDQ7016672.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(5439..>5611)
                     /locus_tag="QBH27_004450"
     CDS             complement(5439..>5611)
                     /locus_tag="QBH27_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HDQ7016673.1"
                     /translation="IDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAA
                     GKYDITRPKAAG"
BASE COUNT         1228 a         1662 c         1655 g         1066 t
ORIGIN      
        1 tctgctcgtt ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa
       61 tgttctcggc ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt
      121 ccgggggatc tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagcccggg
      181 gaccgccgtg ttgctaggat ggttgttctt ggatcagacg ctgagtgcgc ttcaaatcat
      241 cggcgtcctg ctcgtgatcg ggagtatctg gctgggccaa cgttccaacc gcactcctag
      301 ggcgcgtata gcttgccgga agtcgccttg acccgcatgg cataggccta tcgtttccac
      361 gatcagcgat cggctcgttg ccctgcgccg ctccaaagcc cgcgacgcag cgccggcagg
      421 cagagcaagt agagggcagc gcctgcaatc catgcccacc cgttccacgt tgttatagaa
      481 gccgcataga tcgccgtgaa gaggaggggt ccgacgatcg aggtcaggct ggtgagcgcc
      541 gccagtgagc cttgcagctg cccctgacgt tcctcatcca cctgcctgga caacattgct
      601 tgcagcgccg gcattccgat gccacccgaa gcaagcagga ccatgatcgg gaacgccatc
      661 catccccgtg tcgcgaaggc aagcaggatg tagcctgtgc cgtcggcaat cattccgagc
      721 atgagtgccc gcctttcgcc gagccgggcg gctacagggc cggtgatcat tgcctgggcg
      781 agtgaatgca gaatgccaaa tgcggcaagc gaaatgccga tcgtggtcgc gtcccagtga
      841 aagcgatcct cgccgaaaat gacccaaagc gcggccggca cctgtccgac aagttgcatg
      901 atgaagaaga ccgccatcag ggcggcgacg acggtcatgc cccgggccca ccggaacgaa
      961 gcgagcgggt tgagagcctc ccggcgtaac ggccggcgtt cgcctttgtg cgactccggc
     1021 aaaaggaaac agcccgtcag gaaattgagg ccgttcaagg ctgccgcggc gaagaacgga
     1081 gcgtgggggg agaaaccgcc catcagccca ccgagcacag gtcccgcgac catcccgaac
     1141 ccgaaacagg cgctcatgaa gccgaagtgc cgcgcgcgct catcgccatc agtgatatcg
     1201 gcaatataag cgccggctac cgccccagtc gccccggtga tgccggccac gatccgcccg
     1261 atatagagaa cccaaaggaa aggcgccgtc gccatgatgg cgtagtcgac agcagcgccg
     1321 gccagcgaga cgagcaagac cggccgccgc ccgaaacgat ccgacagcgc gcccagcaca
     1381 ggtgcgcagg caaattgcat caacgcatac agcgccagca gaatgccata gtgggcggtg
     1441 acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg
     1501 atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtct gttgggtttc
     1561 acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact
     1621 gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc
     1681 agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc
     1741 tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact
     1801 tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata
     1861 cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca
     1921 gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac
     1981 cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt
     2041 tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg
     2101 tgcttgagga gcaggccggc ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg
     2161 ccgcgataga cgccttcgac gaagccggtc cggacgcagc gttcgagcag ggactcgcgg
     2221 tgattgtcga tggattggcg aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa
     2281 agggtgacga ttgatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat
     2341 gtagacaaca tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg
     2401 ctttttcatg ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc
     2461 cttctggcgc tcctgctgcg gcgtccgctc gtgggccgtg gcgcgggtcg gggtcgtttg
     2521 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     2581 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     2641 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     2701 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     2761 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     2821 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     2881 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     2941 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     3001 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     3061 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     3121 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     3181 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     3241 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     3301 tcagcaatgt cttttatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     3361 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     3421 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     3481 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     3541 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     3601 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     3661 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     3721 ccatatcggt gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa
     3781 gatcgagctg cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc
     3841 gggacaccac atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aacggacatt
     3901 gatcaaccga taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga
     3961 gcaaatccgc tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc
     4021 atgcaccctc ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt
     4081 tgagccaaat gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga
     4141 ttttcgcgaa ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc
     4201 tgacaggcaa ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag
     4261 gttccttcag ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac
     4321 gaattcttgc ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg
     4381 gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg
     4441 tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg
     4501 aaacagacag aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca
     4561 ccgagagcga ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa
     4621 ggcggttgcg tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg
     4681 caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca
     4741 ccgaatgcat aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt
     4801 ctggaaaacc gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg
     4861 tcgcgggttg ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca
     4921 ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg
     4981 atgccggacc gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa
     5041 tggctgcgtc tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga
     5101 cgatgccgaa aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc
     5161 atgagtatca tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg
     5221 gtgatccatt agctgcagga gcagaagagc atacatctgg aagcaaagcc aggaaagcgg
     5281 cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt taagcctttt
     5341 ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca ttgaatataa
     5401 aagataaaaa tgtcttgttt acaatagagt ggggggggtc agcctgccgc cttgggccgg
     5461 gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc agcccagcgc gaccagctcc
     5521 ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg tcgaaccact ggcctctgac
     5581 ggccagacat agccgcacaa ggtatctatg g
//
LOCUS       DAOFSV010000215         5595 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.215, whole genome shotgun sequence.
ACCESSION   DAOFSV010000215 DAOFSV010000000
VERSION     DAOFSV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.215"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            238..993
                     /gene="repE"
                     /locus_tag="QBH27_004451"
     CDS             238..993
                     /gene="repE"
                     /locus_tag="QBH27_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDQ7016674.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            1581..2747
                     /gene="sopA"
                     /locus_tag="QBH27_004452"
     CDS             1581..2747
                     /gene="sopA"
                     /locus_tag="QBH27_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDQ7016675.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            2747..3718
                     /locus_tag="QBH27_004453"
     CDS             2747..3718
                     /locus_tag="QBH27_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDQ7016676.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            4585..5487
                     /locus_tag="QBH27_004454"
     CDS             4585..5487
                     /locus_tag="QBH27_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDQ7016677.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
BASE COUNT         1320 a         1285 c         1444 g         1546 t
ORIGIN      
        1 agagcgcctt gtaggccttt atccatgctg gttctagaga aggtgttgtg acaaattgcc
       61 ctttcagtgt gacaaatcac cctcaaatga cagtcctgtc tgtgacaaat tgcccttaac
      121 cctgtgacaa attgccctca gaagaagctg ttttttcaca aagttatccc tgcttattga
      181 ctctttttta tttagtgtga caatctaaaa acttgtcaca cttcacatgg atctgtcatg
      241 gcggaaacag cggttatcaa tcacaagaaa cgtaaaaata gcccgcgaat cgtccagtca
      301 aacgacctca ctgaggcggc atatagtctc tcccgggatc aaaaacgtat gctgtatctg
      361 ttcgttgacc agatcagaaa atctgatggc accctacagg aacatgacgg tatctgcgag
      421 atccatgttg ctaaatatgc tgaaatattc ggattgacct ctgcggaagc cagtaaggat
      481 atacggcagg cattgaagag tttcgcgggg aaggaagtgg ttttttatcg ccctgaagag
      541 gatgccggcg atgaaaaagg ctatgaatct tttccctggt ttatcaaacg tgcgcacagt
      601 ccatccagag ggctttacag tgtacatatc aacccatatc tcattccctt ctttatcggg
      661 ttacagaacc ggtttacgca gtttcggctt agtgaaacaa aagaaatcac caatccgtat
      721 gccatgcgtt tatacgaatc cctgtgtcag tatcgtaagc cggatggctc aggcatcgtc
      781 tctctgaaaa tcgactggat catagagcgt taccagctgc ctcaaagtta ccagcgtatg
      841 cctgacttcc gccgccgctt cctgcaggtc tgtgttaatg agatcaacag cagaactcca
      901 atgcgcctct catacattga gaaaaagaaa ggccgccaga cgactcatat cgtattttcc
      961 ttccgcgata tcacttccat gacgacagga tagtctgagg gttatctgtc acagatttga
     1021 gggtggttcg tcacatttgt tctgacctac tgagggtaat ttgtcacagt tttgctgttt
     1081 ccttcagcct gcatggattt tctcatactt tttgaactgt aatttttaag gaagccaaat
     1141 ttgagggcag tttgtcacag ttgatttcct tctctttccc ttcgtcatgt gacctgatat
     1201 cgggggttag ttcgtcatca ttgatgaggg ttgattatca cagtttatta ctctgaattg
     1261 gctatccgcg tgtgtacctc tacctggagt ttttcccacg gtggatattt cttcttgcgc
     1321 tgagcgtaag agctatctga cagaacagtt cttctttgct tcctcgccag ttcgctcgct
     1381 atgctcggtt acacggctgc ggcgagcgct agtgataata agtgactgag gtatgtgctc
     1441 ttcttatctc cttttgtagt gttgctctta ttttaaacaa ctttgcggtt ttttgatgac
     1501 tttgcgattt tgttgttgct ttgcagtaaa ttgcaagatt taataaaaaa acgcaaagca
     1561 atgattaaag gatgttcaga atgaaactca tggaaacact taaccagtgc ataaacgctg
     1621 gtcatgaaat gacgaaggct atcgccattg cacagtttaa tgatgacagc ccggaagcga
     1681 ggaaaataac ccggcgctgg agaataggtg aagcagcgga tttagttggg gtttcttctc
     1741 aggctatcag agatgccgag aaagcagggc gactaccgca cccggatatg gaaattcgag
     1801 gacgggttga gcaacgtgtt ggttatacaa ttgaacaaat taatcatatg cgtgatgtgt
     1861 ttggtacgcg attgcgacgt gctgaagacg tatttccacc ggtgatcggg gttgctgccc
     1921 ataaaggtgg cgtttacaaa acctcagttt ctgttcatct tgctcaggat ctggctctga
     1981 aggggctacg tgttttgctc gtggaaggta acgaccccca gggaacagcc tcaatgtatc
     2041 acggatgggt accagatctt catattcatg cagaagacac tctcctgcct ttctatcttg
     2101 gggaaaagga cgatgtcact tatgcaataa agcccacttg ctggcctggg cttgacatta
     2161 ttccttcctg tctggctctg caccgtattg aaactgagtt aatgggcaaa tttgatgaag
     2221 gtaaactgcc caccgatcca cacctgatgc tccgactggc cattgaaact gttgctcatg
     2281 actatgatgt catagttatt gacagcgcgc ctaacctggg tatcggcacg attaatgtcg
     2341 tatgtgctgc tgatgtgctg attgttccca cgcctgctga gttgtttgac tacacctccg
     2401 cactgcagtt tttcgatatg cttcgtgatc tgctcaagaa cgttgatctt aaagggttcg
     2461 agcctgatgt acgtattttg cttaccaaat acagcaatag taatggctct cagtccccgt
     2521 ggatggagga gcaaattcgg gatgcctggg gaagcatggt tctaaaaaat gttgtacgtg
     2581 aaacggatga agttggtaaa ggtcagatcc ggatgagaac tgtttttgaa caggccattg
     2641 atcaacgctc ttcaactggt gcctggagaa atgctctttc tatttgggaa cctgtctgca
     2701 atgaaatttt cgatcgtctg attaaaccac gctgggagat tagataatga agcgtgcgcc
     2761 tgttattcca aaacatacgc tcaatactca accggttgga gatacttcgt tatcgacacc
     2821 agctgccccg atggtggatt cgttaattgc gcgcgtagga gtaatggctc gcggtaatgc
     2881 cattactttg cctgtatgtg gtcgggatgt gaagtttact cttgaagtgc tccggggtga
     2941 tagtgttgag aagacctctc gggtatggtc aggtaatgaa cgtgaccagg agctgcttac
     3001 tgaggactca ctggatgatc tcatcccttc ttttctactg actggtcaac agacaccggc
     3061 gttcggtcga agagtatctg gtgtcataga aattgccgat gggagtcgcc gtcgtaaagc
     3121 tgctgcactt accgaaagtg attatcgtgt tctggttggc gagctggatg atgagcagat
     3181 ggctgcatta tccagattgg gtaacgatta tcgcccaaca agtgcttatg aacgtggtca
     3241 gcgttatgca agccgattgc agaatgaatt tgctggaaat atttctgcgc tggctgatgc
     3301 ggaaaatatt tcacgtaaga ttattacccg ctgtatcaac acagccaaat tgcctaaatc
     3361 agttgttgct cttttttctc accccggtga actatctgcc cggtcaggtg atgcacttca
     3421 aaaagccttt acagataaag aggaattact taagcagcag gcatctaacc ttcatgagca
     3481 gaaaaaagct ggggtgatat ttgaagctga agaagttatc actcttttaa cttctgtgct
     3541 taaaacgtca tctgcatcaa gaactagttt aagctcacga catcagtttg ctcctggagc
     3601 gacagtattg tataagggcg ataaaatggt gcttaacctg gacaggtctc gtgttccaac
     3661 tgagtgtata gagaaaattg aggccattct taaggaactt gaaaagccag caccctgatg
     3721 cgaccacgtt ttagtctacg tttatctgtc tttacttaat gtcctttgtt acaggccaga
     3781 aagcataact ggcctgaata ttctctctgg gcccactgtt ccacttgtat cgtcggtctg
     3841 ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
     3901 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accatggtcc cactcgtatc
     3961 gtcggtctga ttattagtct gggaccatgg tcccactcgt atcgtcggtc tgattattag
     4021 tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc
     4081 actcgtatcg tcggtctgat tattagtctg ggaccacgat cccactcgtg ttgtcggtct
     4141 gattatcggt ctgggaccac ggtcccactt gtattgtcga tcagactatc agcgtgagac
     4201 tacgattcca tcaatgcctg tcaagggcaa gtattgacat gtcgtcgtaa cctgtagaac
     4261 ggagtaacct cggtgtgcgg ttgtatgcct gctgtggatt gctgctgtgt cctgcttatc
     4321 cacaacattt tgcgcacggt tatgtggaca aaatacctgg ttacccaggc cgtgccggca
     4381 cgttaaccgg gctgcatccg atgcaagtgt gtcgctgtcg ccggcctcct cacccggtca
     4441 cgtttcgtcg ttcctcctcc acgcgctgcg gcttcggggc cgcacctgca ttcgtatgcg
     4501 gtcgcccggt tacaggtgcg gcacggcctg atggaggccg catgtgagag gagaattccc
     4561 atgccaaact ggtgctcgaa tcgtatgcat ttttctggtg aaccggcaca gattgctgag
     4621 attaaacgac tggccagcgg tgccgtcaca ccgctttatc gccgcgccac aaatgaaggt
     4681 attcagctgt ttctggccgg aagtgccgga cttctgcaga ccactgaaga tgtgcagttt
     4741 gaaccgtgcc ccggactgac ggatgccgga cgtggtgttg tatcgccgga gaatatcgcg
     4801 ttcacccgct ggctgacaca cctgcagaac ggtgtactgc tggatgagca aaactgcctg
     4861 atgctgcatg aactctggct gcagagtggt actggccagc gtcgctggga aggattaccg
     4921 gatgaggtca gggaaaccat caccgtgcat ttcaccgcaa aaagaggtga ctggtgtggc
     4981 ttctggagta acgaggatgt atcggtgtgg tggaaccgtc tgtgtgacaa tgtactgccg
     5041 gaaaaaacca tgccgtttga cctgctgacg gttctgccca cccgcctgga tgttgaagtg
     5101 aatggcttta acggtggtgt tctgaacggt gttccttctg cgtatcactg gtatacggaa
     5161 cagtatggcg tgaagtggcc tgtggggtat gaggtgaata tcagccgtca gggagaaaac
     5221 ttcattcaag tggatttcga cacgccgtgg tgtcagccgg aaagcaacgt tgttgcagaa
     5281 ttaagccgcc gtttcggctg cacgctggag cactggtacg ccgaacaggg ctgcaatttc
     5341 tgtggctggc agcgatatga gcgtggagag cttgttgatg tgctgtgggg ggaactggaa
     5401 tggtcttccc cgacagatga tgatgagctg ccggaagtca ccgcgccgga gtggatagtc
     5461 gacaaggtgg cgcattacgg gggatgaagt atgacggaga cgggcgggca accgcccgtt
     5521 tcttttccga caaaggatgt cgccgtgctc ctctttttac tgcgccggct gacgcggcgc
     5581 ggcaggaacg ctgcc
//
LOCUS       DAOFSV010000216         5317 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.216, whole genome shotgun sequence.
ACCESSION   DAOFSV010000216 DAOFSV010000000
VERSION     DAOFSV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.216"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="QBH27_004455"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="QBH27_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ7016678.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="QBH27_004456"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="QBH27_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDQ7016679.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="QBH27_004457"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="QBH27_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDQ7016680.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="QBH27_004458"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="QBH27_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDQ7016681.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="QBH27_004459"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="QBH27_004459"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDQ7016682.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3777..4136)
                     /gene="tusC"
                     /locus_tag="QBH27_004460"
     CDS             complement(3777..4136)
                     /gene="tusC"
                     /locus_tag="QBH27_004460"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDQ7016683.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4136..4522)
                     /gene="tusD"
                     /locus_tag="QBH27_004461"
     CDS             complement(4136..4522)
                     /gene="tusD"
                     /locus_tag="QBH27_004461"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDQ7016684.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4522..5244)
                     /locus_tag="QBH27_004462"
     CDS             complement(4522..5244)
                     /locus_tag="QBH27_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ7016685.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
BASE COUNT         1321 a         1371 c         1285 g         1340 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcgc gacctttggt
      301 cagagaacgc agctgagttg catatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttcagg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgattgcagc tgcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtacacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgctcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccagat atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatac gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaaca ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaagt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aattcgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agagcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt gccgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtcac cttcactgag cagacgcagc
     3721 agcgcagcaa agtccgtcag ccagggtgaa cgatgtaatg tgtgcagcat aacgcctcaa
     3781 aacctcaaaa taacatcgta gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc
     3841 gtggcttcga ccacaaaggg tgtctgcgga tctaacccac gttcgcgcag tgaagccgcg
     3901 caaatccagc actgttcaat atcgtacaga cccaacaatt taaaagtggc aatgtaatca
     3961 cgcgccagca ctgcatcggg cttttgtcct ggcagcagct gaaaaacgcc atcagcaata
     4021 aagaagacgg ccagatcgtc agttaatgcg gaagttgcca gtaaagcatc taaaccttcc
     4081 cggcctgcag ttgtaccatg aggcgcagta gaaaaaacaa acgcaattcg tttcatcaga
     4141 actgtaccac cctgtcacag gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg
     4201 taaatcccgg ctgaaggttt gacgaagcaa gccccagtct tccggcctcc gtttcatcaa
     4261 caacgccacg gcgtaatgct gccgctacgc agatattcag cgccacacca tgttgcgcat
     4321 tcaactgttg ccagccgcgc acaaggtcaa attcgtcgct tgccggtgag gtcaattggt
     4381 tagcgttata gaccccttcc cgatagaaaa aaacgctgct taactcatgg ccttctgcta
     4441 tcagcgcctg cgcaaactga aaagcactac tcgcctgttg cgtaccgtat gctggcccgg
     4501 tcaccacgat ggcaaaacgc attacttatc ttgcccctgg aaatcaccgc tcttgaactg
     4561 gcggatgtag agatagacag tgtgtttgga gatgttcagg cggtcagcaa cctggttgat
     4621 cgcgtcttta atatcgaaga tccctttctc gtagagattt agcacgatct gacgattttt
     4681 ggcgttatta gaaacattgc gatcggcatt cacttcttcg atggtgaact ccagcgtttg
     4741 ggtaaccaga tcttcaacag aagaggcaaa gttgacgctt gaaccgacat ccggagtttc
     4801 cggcggcaca aaagtgctca taatctgcga gaagggaaca tcaagattca tattgatgca
     4861 cagcagacca attacacgct gttcgcggtt acgaatcgcg atagtcaggg acttcattaa
     4921 tacgccgctt ttggcgcgag taaagtagca tttagaaacg ctgctatccg ctcccgtcat
     4981 atcgtgcagc atacgtagcg ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg
     5041 ttcaccgtta gcaatgcgaa tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat
     5101 ttcacagtgg gagccaataa gcatcgctaa cccgtccacc accgcttcgt aggatttcag
     5161 aatatcaaaa tcggtctggt caaaaggacg ttgatccagt aaatccaact cactggtttc
     5221 gttggttaaa agcgacctgg acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa
     5281 cttttcaggg caggctcatt aatgatgcgg gtaacta
//
LOCUS       DAOFSV010000217         5305 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.217, whole genome shotgun sequence.
ACCESSION   DAOFSV010000217 DAOFSV010000000
VERSION     DAOFSV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.217"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1159
                     /gene="emrY"
                     /locus_tag="QBH27_004463"
     CDS             <1..1159
                     /gene="emrY"
                     /locus_tag="QBH27_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDQ7016686.1"
                     /translation="QSLLLRNYPPEKRTFALALWSMTVIIAPICGPILGGYICDNFSW
                     GWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRD
                     LDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLF
                     YSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRLLVTFSF
                     LMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANAS
                     SMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSL
                     SGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
     gene            complement(1267..2595)
                     /gene="dsdA"
                     /locus_tag="QBH27_004464"
     CDS             complement(1267..2595)
                     /gene="dsdA"
                     /locus_tag="QBH27_004464"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDQ7016687.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKASQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(2613..3950)
                     /gene="dsdX"
                     /locus_tag="QBH27_004465"
     CDS             complement(2613..3950)
                     /gene="dsdX"
                     /locus_tag="QBH27_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HDQ7016688.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            4168..5103
                     /gene="dsdC"
                     /locus_tag="QBH27_004466"
     CDS             4168..5103
                     /gene="dsdC"
                     /locus_tag="QBH27_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HDQ7016689.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHNFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHVGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
BASE COUNT         1445 a         1240 c         1231 g         1389 t
ORIGIN      
        1 acagagttta ttattaagga attatccacc agaaaaaaga acatttgctc tggcattatg
       61 gtcaatgacc gtgattatcg ctccgatatg tgggccgata ttgggcggct atatttgcga
      121 taactttagc tggggttgga tatttttaat caatgtccct atggggatta tcgtcctgac
      181 attatgctta accttactta aaggacgaga aactgagact tcaccggtca aaatgaatct
      241 accaggactg accctgttag tgctcggtgt tggtggcttg caaattatgc ttgataaagg
      301 gcgcgatctg gattggttca actcgagtac aataatacta ctaacagtag tatcagttat
      361 ttctctgatc tctttagtca tttgggagtc gacctcagag aatccgattc ttgacctcag
      421 tttgtttaag tcccgtaatt tcaccattgg tattgtgagt atcacatgcg cgtatttatt
      481 ttactctgga gcgatagttc ttatgccgca gttactccag gaaacgatgg ggtataatgc
      541 gatatgggcc ggacttgcct atgcgcccat cggtatcatg ccgctattaa tatcaccttt
      601 gataggacgt tatggcaaca aaattgatat gcggttgttg gtgacattta gttttttgat
      661 gtatgcggtt tgctattact ggcgttctgt gacatttatg ccaacgattg attttacagg
      721 catcattttg ccacagtttt ttcagggatt cgccgttgcc tgtttctttt tacccttaac
      781 aacgatttcg ttttcaggct tgccagataa taaatttgcc aatgcctcga gtatgagtaa
      841 tttttttcgc accttgtcag gatcagttgg tacgtcgttg acaatgacac tgtggggacg
      901 ccgcgaatcg ttacaccata gtcagttgac agcaaccatc gatcaattta accccgtgtt
      961 taattcatcg tcacaaatta tggataaata ctatggttcg ctttcaggag ttcttaatga
     1021 aattaataat gaaataactc agcagtcact ttctatttct gcaaatgaga ttttccgtat
     1081 ggcggctatt gcttttatct tacttacggt tttggtttgg tttgcgaaac cgccgtttac
     1141 agcgaaaggc gttgggtgat ggaaaaagag gaggctatag caggtaaaac ggcagaatct
     1201 taagtctgcg tcatcggcag catcgctcac ccagggaaag gatggtgatg ctgcgttgaa
     1261 acgttattaa cggccttttg ccagatattg attcatctct tcttccggca ccattccacc
     1321 tcccgtcgcc cacaccagat gagtggcatt acgcagttgt tctgcgctga aaccgtgcat
     1381 ctgttggtaa cttactgatg cgcacacgcg ctgaggtccg gccatacccg ccagtgccga
     1441 aggttcaaga cgaatacctt cttcctgcgc cagccagcca agcatgtcat acatggtttg
     1501 atcgctaagg gtatagaagc catccagcag acgctccatc gcccgcccga caaagcccga
     1561 tgcgcgacca accgcaaggc catcggcagc ggtaaggttg tcgatgccaa tatcctgaac
     1621 agaaatctga tcgtgtaatc cggtatggac gcctaataac atacacgggg agtgtgttgg
     1681 ttcggcaaaa aagcagtgaa catgatcgcc aaacgccagt ttaagtccga atgcgacgcc
     1741 accaggacca ccgccaacac cacacggcag atagacaaat agagggttat cagcatcgac
     1801 gatacggcct tgctgggcaa attgcgcttt aagacgctgg ccagcgacgg aatacccaag
     1861 gaacaacgtg cgggaattct cgtcatcaat aaagaaacag ttcgggtcag actgcgatgc
     1921 tttacgtcct tcctcgacag caacaccata atcttgctca tattccacga ccgtaacgcc
     1981 atggctgcgc agtttcgctt ttttccatgc ccgggcatca gcagacatat gcactgtcac
     2041 cttaaagcca atgcgggcgc tcataatacc gattgataac cccagatttc cggttgagcc
     2101 cacagcaatg ctgtattggc taaagaactg tttaaactcc ggagaaagca gtttgctgta
     2161 gtcatcttca agcgtcagca accctgcttc cagagccagt ttttctgcat gtgccaggac
     2221 ttcataaatc ccgccgcgtg cttttatgga gccggaaata ggcaaatggc tatctttttt
     2281 cagtaacagt tgcccgctga tcggttgctg atattccttt tccagccgtt tttgcatagc
     2341 cggaatggca accagttctg attcaataat ccccccagtg gcagcagttt caggaaatgc
     2401 ttttgccaga tagggggcaa aacgagataa gcgcgcatgg gcgtcctgaa catcctgttc
     2461 ggtcaggcca acataaggta aaccttcagc caatgaggtc gtgccaggat tgaaccaagt
     2521 ggtttcttta agagcaacca gatcctttac caacggatac tgggcgatga gcgaattcat
     2581 tttagcgttt tccatagtac gtctctttgc gcttagatga taaatgacag caggaatgtg
     2641 cccgccagag cgatgaccga agcgataaat gtcgctgtcg tatagtattt aaatgtttca
     2701 ttgagcgtag cgccgcaata ttgcttcacc agccagaaaa gcgagtccgt aacgatcgtg
     2761 cagccaatcg caccggaacc gatagcaatc gcaataattt ccgggctgat gtcgggatac
     2821 agcggcagca tgggggcaac aattgccgtt gcccccatca ttgccaccgt agcggagccc
     2881 actgcagcat gcagaataag tgccaccaac caggccagaa gaatcgggtg catatgcata
     2941 ttggagagaa taactgccag cgtatcagcg agactgctgc tttttaaaat ggcgttgaat
     3001 gcgcctccgg ccccgataat cagcaaaata ttagcaatag aaccgaaacc attttccgta
     3061 tgtgtgagca tcgtccccat gctcatatgc tgacgtatac ccaacacata ataggcgaca
     3121 aacacggcga taaacatggc agtgataggg ttgccaataa actcaagcaa ggtatacaaa
     3181 ccactctcac gcgccatatt caattcggca atcgttttaa ccaacatcag cgcaatgggt
     3241 agcagtatgg tgaataacgt tgcgcctaat gaaggtagtg ttttttcatc gcgaactttg
     3301 agatcggcaa actctgtggg tacaggttta aagggcagtc gttgccccag aaatttaagg
     3361 aaaagtgggc caccgatcag tgatgccatc agcccaacca gcaaaccgta gacgatcacc
     3421 gaaccgatat ctgcgcccag cttattggca acatataaag cagccggatg tgggggaacc
     3481 acgcagtgca cagccatcaa tgcggtacac agcggaatgg ccagctttaa caatgaggta
     3541 ttggtttttt tggcaattga aaaagccaga ggaatcaata gcacgacgcc cacttcaaca
     3601 aacagcgtga tgccgcaaat caggccaacc agcaccataa tgacatcagc cgaaagccac
     3661 cggcagcgtt gaagtgtcag accaattctt tctgcggccc cggatacttc catcattttt
     3721 cccagtatcg tgccaaggcc gataaccgca gcgaggaacc ccagcgttcc gccaattcca
     3781 ctttcaatag catttaccat atccagtggc cccataccca tcatcgttcc cacgaagaag
     3841 ctggccagca acagcgccag aaacgggtgg aatttgaact tcacgatggt cagtacaatt
     3901 aacacgatgc tgataagcag cgtgctcaca acccagattt gagagtgcat atctcacctt
     3961 cccctgtgat taacctgatg atattggaca aaaaaacaac gcaggctgac aaacgataaa
     4021 atttgccttt cacatgagct aaattgcatc aagggagtga tttacatcta aaaatgaagg
     4081 tgaattgaga tatggttcac tttagctcac cttacagctg aaattttgcc gctatttttt
     4141 tacacttaat agaaaaatga ggtgattatg gaaccccttc gtgaaataag aaatcgactg
     4201 cttaacggct ggcaactatc aaaactgcat acttttgaag tggctgccag gcatcagtct
     4261 ttcgccctgg cggcagagga attgtcgctg agccccagtg cggtaagtca ccgtatcaat
     4321 cagctggaag aagaattagg tattcagttg tttgttcgtt cccatcgcaa agtggaatta
     4381 acgcacgagg ggaaacgcgt ttattgggcg ctaaaatcgt cgctggatac cctgaatcag
     4441 gaaattctgg atatcaaaaa tcaggagtta tcgggaacgt taactctgta ttcccggccc
     4501 tctatcgccc aatgctggtt ggtgcccgca ttaggtgact ttacacgccg atatccgtct
     4561 atttcgctca ccgtgctcac tggtaatgac aatgtcaatt tgcaacgtgc cggaatcgat
     4621 ttggcgatct actttgatga tgcgccgtca gcgcaactga cccataactt tctgatggat
     4681 gaagaaatct tgccagtttg tagcccggag tacgctcata gacatgattt aaccaacacg
     4741 gtaattaacc tgcgtcactg tacgttgctc catgacagac aggcatggag caacgactcc
     4801 ggtacggatg aatggcatag ttgggcgcaa cattatgcgg ttaatttgcc gacatcttct
     4861 ggaattggct ttgatcgttc tgatttagcc gttatcgccg cgatgaatca tgttggggtg
     4921 gcgatgggaa ggaaacgcct ggtacaaaaa aggcttgcca gtggtgagct cgtcgcgccg
     4981 tttggcgata tgacggtgaa atgccatcag cattattaca tcaccacatt accgggcagg
     5041 cagtggccaa aaattgaggc atttattact tggttaagag aacaggtaaa aaccacttca
     5101 taatgaataa gaccacgagt tcctggtgtg ccttattcgg cactgctagt gcgaatttaa
     5161 taatttacgg tttttacgtg gttttaggga taaacacaag aatggtgtcc cctgcaggaa
     5221 tgtgattaat gaattaagaa attgattttt atgaatataa aatttttatt gtcataattc
     5281 tatacactta gttatacaca taaga
//
LOCUS       DAOFSV010000218         5275 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.218, whole genome shotgun sequence.
ACCESSION   DAOFSV010000218 DAOFSV010000000
VERSION     DAOFSV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.218"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..331)
                     /locus_tag="QBH27_004467"
     CDS             complement(<1..331)
                     /locus_tag="QBH27_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016690.1"
                     /translation="MKRAILLVLIAAGLLAGCGEKTPKCSSDDAKNLVGDIARKTIEK
                     GMKLDKDVRISVENVRTISHESDLDVYQCAADLTFTKPDLQNSLPITYRIQKTDEGKG
                     PFYINVTG"
     gene            complement(362..721)
                     /locus_tag="QBH27_004468"
     CDS             complement(362..721)
                     /locus_tag="QBH27_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDQ7016691.1"
                     /translation="MNRKLLALFMAAITLPVAAAQYVKPGAVLTLHPGQNNAEFNINA
                     STGDGSGVCNMEGTAQQIAAGESQKRRWVWNDNASECVAVISELTNGKASIMTRGCEG
                     YCGASAAGAMDGLFNKK"
     gene            complement(736..1263)
                     /locus_tag="QBH27_004469"
     CDS             complement(736..1263)
                     /locus_tag="QBH27_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016692.1"
                     /translation="MATTITEITECFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLALGKITKNIRIT"
     gene            complement(1275..1544)
                     /locus_tag="QBH27_004470"
     CDS             complement(1275..1544)
                     /locus_tag="QBH27_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7016693.1"
                     /translation="MRNSTASQKKQIKGSSGNWHRADIVAALHKRGLTLALLSRSHGL
                     APRTLNNALERHYPRAERIIAGALDTTPETLWPERYALKNGPNSR"
     gene            1666..2154
                     /locus_tag="QBH27_004471"
     CDS             1666..2154
                     /locus_tag="QBH27_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016694.1"
                     /translation="MSSKRTVSAIPTHPEASVEEAKDWLTAEEFTHIEGMPGTARGAR
                     MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGEAFTLNDSDRQLSLW
                     VQLYRNMSPASRAQLMEQALRLVKDDLMSQGPNVSELAVQIMKLPEDERKRLFEKLNG
                     KV"
     gene            2509..2901
                     /locus_tag="QBH27_004472"
     CDS             2509..2901
                     /locus_tag="QBH27_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HDQ7016695.1"
                     /translation="MPVDQELINIILNEAGNPPPHKAKITAVSLLFKDLSYSAEKGGY
                     HPVEIRIISRNDEWYFDYITDFSYMGTVYPELEKEIDISWSQQYVFLAYVGDLPVNAG
                     RELFELWQSNFIQYHGMNIYTITVLWES"
     gene            3021..3482
                     /locus_tag="QBH27_004473"
     CDS             3021..3482
                     /locus_tag="QBH27_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016696.1"
                     /translation="MLSWLYDGRVKRRPLMNWLIQTYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYRRQFPVEITRPFAGKRGLTDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WSLLVKMDEEMLCFFDSNGRTTMPRKAFSLRTGVTRRQLFPDAIYFIEREF"
     gene            3484..3936
                     /gene="radC"
                     /locus_tag="QBH27_004474"
     CDS             3484..3936
                     /gene="radC"
                     /locus_tag="QBH27_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDQ7016697.1"
                     /translation="MTMFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNHNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPSRCDIQLTKKLQELLEILDVRLLDHFIVAGTETVSMAERGLV"
     gene            3983..4912
                     /locus_tag="QBH27_004475"
     CDS             3983..4912
                     /locus_tag="QBH27_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ7016698.1"
                     /translation="MSRTALRQVVPVVQTTVFDAYIVGLAPSGRRGITSLLNRSASIL
                     KHGADAADYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNYMDA
                     ETLARIRSVKRVSGDVQRKGRALGRQEIRVLIQAAKQHPQSVRRCRDVAIVLMLCGTG
                     LRAGELVKLERRDYDNGILTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     RRIQRSGKVASQPLTTTGLTGILAELQQTAGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGDTMKISASKRIRCF"
     gene            complement(4899..>5275)
                     /locus_tag="QBH27_004476"
     CDS             complement(4899..>5275)
                     /locus_tag="QBH27_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016699.1"
                     /translation="EGEPNGIYRPEEHPMIVPARSPKALEIYTVYQLVKGRFDKLHTG
                     ESDREKYASVAEKLNEYGIWKPRLKRLSDGKEWAREDVAWMLQPENINAQINIQNQKY
                     ARLSAEDETETESGNASTAKNI"
BASE COUNT         1299 a         1280 c         1297 g         1399 t
ORIGIN      
        1 aacctgttac gtttatataa aacggacctt taccctcatc agttttctga atccggtagg
       61 tgattggtag agaattttgc aaatctggtt ttgtaaacgt taaatcagca gcgcactgat
      121 aaacatccag atctgattca tggctaattg ttctgacatt ttctacagaa atacgcacat
      181 ccttatccag tttcatccct ttctcgattg tctttcttgc aatatccccg accagatttt
      241 ttgcatcatc actactacat tttggcgttt tctcgccgca gccagccagc aaaccagcag
      301 caattaatac cagaagtatt gctcttttca tctgtagttc cttcgtaaat taatgatact
      361 tttatttctt attaaataga ccatccattg cgcctgccgc agaagccccg cagtatccct
      421 cgcaaccacg tgtcataatg ctggccttac cgtttgtcag ttcactaatt actgcaacac
      481 actcgctggc attgtcattc cagacccagc gacgcttctg actttctccg gcagctattt
      541 gctgtgctgt cccctccata ttgcatacgc ccgaaccatc ccccgttgag gcattaatgt
      601 tgaattcagc gttattctgt cccggatgca gggttagaac cgctccgggt tttacgtact
      661 gcgctgctgc gactggtagc gttatggctg ccatgaataa ggccaacagt tttctgttca
      721 ttaattaatt ccctgttaag tgatacggat gtttttggtg attttcccca gtgccagcgg
      781 tctgcgtttt tccacaaaga aataaacgac gttgaatgca gacttgcgat gattccctcg
      841 accaagagag ggccagttgc ggaaggattc aatctgttct tctgaatagg gtgtgtcaga
      901 gaaatcaaac agcacaagca aggctttgcc gtcatgtttc atcaatttct ttacttctgt
      961 actgttgacg gtcgcagaga cattggaacg tgccgcagtt ggtttacgta ctgcaaactc
     1021 aacggccaca tcgtcaatga caaaatcaat gtaaccgtgc ccggatacag ttcctggcag
     1081 tttgcttttt acttccggtg agacgttatc tgcaaaccaa cccagcaaat agcaacgtac
     1141 cagaggcagt aattcccgct cgctacattc gttcagacgc agggttttga cgaactccct
     1201 gcgataaaga gaactgaaaa aatactcaaa gcattccgtt atttcagtga tggtggttgc
     1261 catggttgca tcctttacct gctattaggt ccatttttaa gggcatacct ttccggccat
     1321 aatgtttccg gtgtcgtatc cagagccccg gcaataatgc gctcagctct gggataatga
     1381 cgctctaagg cgttatttaa tgtccgtggc gcaaggccgt gactgcgtga cagtaacgcc
     1441 agcgttaacc ctcgcttgtg aagtgctgcc acaatatcgg cacgatgcca gttccctgaa
     1501 cttcctttga tttgcttttt ctgcgatgct gttgagttac gcacagtatt aactccatgg
     1561 aatcagtaca tggatattaa atgcgaataa aagatacctt tcaagttctt ttggtatcta
     1621 tttattggtt ttcatattaa cgcattgata tctggtgaaa tttatatgag cagtaagaga
     1681 acagtttcgg ctatacctac tcatcctgaa gcttctgttg aagaggctaa agactggctg
     1741 acagcagagg aattcactca tattgagggg atgccgggca ctgcgcgtgg cgccaggatg
     1801 cgcttggaaa agctggcatc cctgcacccg gaaatacgtc gtaaaaggac tgccgggaaa
     1861 ggtgtggttt accatattag cgcagccaca atgtcgcttg atgatgagag aaacagggct
     1921 aagggcgaag cgtttactct caacgacagt gatcgccagc taagcctctg ggtgcagctg
     1981 tatcgcaata tgtcgccagc ttcgcgggcg caactgatgg agcaggccct caggctggta
     2041 aaggacgatc tgatgtctca ggggcccaat gtttctgaac tggcggtaca aatcatgaag
     2101 ctgcccgaag atgagcgtaa gcgcctcttt gaaaagctga acgggaaggt gtgatagcgt
     2161 cagttttccg ccatgccaga cagaaggaac caccccggaa tcctgacctg tctgacatgg
     2221 cagaatggaa agtatgccat cacttaataa tgaataaaaa cctattgcca gttatttcat
     2281 tgctgaacca catattagtt tctctttgtt actatttgca taaaaagtat atttttgata
     2341 taataatatt gctggtgaac tatattttac ccagtcagta cgttgtcatg cacttaccat
     2401 ttattaggtg attaaacccc acctgtgctg gtgcagacat aacgtcttat cagaatatat
     2461 tgaacaggaa aatcactgat gaatattgtc catcaggaag ggctagctat gcccgtcgat
     2521 caggaattaa taaatatcat attaaatgaa gcgggaaatc caccgccgca taaagccaaa
     2581 attacagccg tcagtctgtt atttaaagac ctgagttaca gcgctgaaaa gggtggttac
     2641 catcctgtcg aaatacggat tatttcacgc aatgatgaat ggtattttga ttacattacc
     2701 gacttcagtt atatggggac ggtatacccc gaacttgaaa aggaaattga tatcagctgg
     2761 agtcagcaat atgtctttct tgcttatgtt ggtgacctgc ctgttaacgc cggaagggag
     2821 ctgtttgaac tctggcaatc caactttatt cagtatcacg gcatgaacat ctacaccatt
     2881 accgttctct gggaaagcta atccccctgt actaaccatc actaatttat ggggaattat
     2941 ctgtgacgac tgacagaggg atacgtgcgt ctcaacaggg cgaactggac tgtctttgcg
     3001 gtgcttattg cgtggttaac atgctgagct ggctgtatga cggcagggtg aaacgacgcc
     3061 cgttaatgaa ctggcttatt cagacttatc agcagcgctg gcctttgcat gaatggctga
     3121 ctgaaggaat tgaagaagac cgcttagact ggttaatagc tcaggtgttg cagaaagggc
     3181 attaccgccg ccagttcccg gttgaaatca ccagaccctt tgcagggaag cgggggttaa
     3241 cagacgggcg gctgttcagg gaaatgcagc ggtttcttga cgtcactgac cactctcgcc
     3301 tgattatgct cagcgaccag tttcactggt cccttctggt caaaatggac gaagaaatgc
     3361 tctgcttctt cgacagcaac gggcgaacca ccatgccccg taaagctttc tccctgcgta
     3421 ctggcgtaac ccgccgccag ctgtttccag acgccattta cttcattgaa cgggagttct
     3481 gacatgacca tgtttaccca gcgtgaaacc cgtatccttg accaggcgcg tgacattatt
     3541 agccggtact accagcgtgg ggttcaactc tgttcccctg atgatgttcg acggtgcgtg
     3601 atggttgagc tggcaccgct tgaacatgaa gagtttggca tcatcctact cgataatcat
     3661 aatcagctgc tgcaccgcga aatcctgttc aggggtacgc taaactcggt cagcgttcac
     3721 ccccgtgaag ttattaagcg tgtgctgaaa cataacgccg ccgccgtcat ccttgtccat
     3781 aatcacccca gcggcgagcc tgaacccagc cggtgtgaca tccagttgac caaaaagctt
     3841 caggagctgc tggaaatact ggatgtgcgg ttacttgatc actttatcgt ggcaggaact
     3901 gaaacggtgt cgatggctga gcgaggactg gtgtgatgct gaaagttact tggtttaatg
     3961 tacgttattg cgggttacgc gcatgagcag gacagcgttg cgtcaggtcg tgccggtggt
     4021 gcaaaccacc gtgttcgatg cgtatattgt cggtctggcc ccctcgggac ggcgaggtat
     4081 caccagcctg ttaaaccgca gcgccagcat acttaaacac ggtgcggatg cagcagacta
     4141 tccgtgggaa caacttaact acgccgccgt tgccaaagtg cgcgcggcac tgctggatga
     4201 cggttacgcc gtgtcatcgg tcaacatggc gctgtcagca ctccggggcg tagcgcagac
     4261 ggcctttaac ctcaattaca tggatgccga aacgctggca cgtatccggt cagttaagcg
     4321 cgtgagcggc gatgtccagc gcaagggaag ggcgctgggc aggcaggaaa tccgggtact
     4381 gattcaggct gcaaaacagc atccgcaatc tgtacggcgc tgtcgcgatg tcgccattgt
     4441 gctgatgctt tgcggaacgg gcctgcgggc cggggaactg gttaaacttg agcgccggga
     4501 ctatgacaac ggcatcctga cggtgaggca agggaagggg cgtaaatacc gtgaaattca
     4561 cgtggcggat gcggtggaca aggctattcg tgcgtggctg aaagttggcg cagacgaggc
     4621 tgacagtgcg ttgtttaggc gaattcagcg cagtggtaag gtcgcaagcc aaccgctgac
     4681 tacgaccggg ttaacgggta tcctggctga gttacagcaa acggcaggta tagcacgttt
     4741 taccccgcat gatatgcggc gcaccttcat cacccgtttg ctggagcagg gcgtggatat
     4801 caataccgtc cgccagttgg caggccacag cgacatatcc acgacgacgc gctatgactg
     4861 tcgtggtgat actatgaaga tcagtgccag caagcgcatc agatgttttt agcagttgag
     4921 gcgttgccag actcggtttc agtttcatct tcagcactca accgcgcgta cttttgattc
     4981 tggatgttaa tttgtgcatt gatattttca ggctgaagca tccacgcaac atcctcacgc
     5041 gcccattctt taccgtcaga gagtcgtttt aaccggggtt tccatattcc atactcatta
     5101 agtttttctg ctaccgaagc atatttctca cgatctgact cccccgtatg aagcttgtca
     5161 aaacgacctt ttacaagctg gtaaacagta tagatttcca gtgccttagg ggacctggcc
     5221 gggactatca tggggtgttc ctccggccta taaatcccgt ttggctctcc ctcat
//
LOCUS       DAOFSV010000219         5258 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.219, whole genome shotgun sequence.
ACCESSION   DAOFSV010000219 DAOFSV010000000
VERSION     DAOFSV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.219"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            42..3164
                     /gene="ag43"
                     /locus_tag="QBH27_004477"
     CDS             42..3164
                     /gene="ag43"
                     /locus_tag="QBH27_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDQ7016700.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPCETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDIRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASS
                     DNNDFRARGRGWQGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGNSAEGY
                     NGQATLNVTF"
     gene            3259..4386
                     /locus_tag="QBH27_004478"
                     /pseudo
     CDS             3259..4386
                     /locus_tag="QBH27_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4462..4917
                     /locus_tag="QBH27_004479"
     CDS             4462..4917
                     /locus_tag="QBH27_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016701.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            4996..5229
                     /locus_tag="QBH27_004480"
     CDS             4996..5229
                     /locus_tag="QBH27_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDQ7016702.1"
                     /translation="MLGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEADAGKQLNAWLASGGLLRQ"
BASE COUNT         1258 a         1396 c         1534 g         1070 t
ORIGIN      
        1 cggctttttt attcaccctc actctgatta aggaaatgct gatgaaacga catctgaata
       61 cctgctacag gctggtatgg aatcacatta cgggcgcttt cgtggttgcc tccgaactgg
      121 cccgcgcacg gggtaaacgt ggcggtgtgg cggtggcact gtctcttgcc gcggtcacgt
      181 cactcccggt gctggctgct gacatcgttg tgcacccgtg tgaaacagtg aatggcggaa
      241 cactggtaaa ccatgacaac cagtttgtat ccggaacagc tgatggcgtg actgtcagta
      301 ccgggcttga gctggggccg gacagtgacg aaaacaccgg cgggcaatgg ataaaagcgg
      361 gtggcacagg cagaaacacc actgtcaccg caaatggtcg tcagattgtg caggcaggag
      421 gaactgccag tgatacggtt attcgtgatg gcggagggca gagccttaac ggactggcgg
      481 tgaacaccac gctggataac agaggtgagc agtgggtaca cgggggaggg aaagctgccg
      541 gtacaattat taaccaggat ggttaccaga ccataaaaca tggcggactg gcaaccggaa
      601 ccatcgtcaa caccggtgca gaaggtggtc cggagtctga aaatgtgtcc agcggtcaga
      661 tggtcggagg gacggctgaa tccaccacca tcaataaaaa tggccggcag gttatctggt
      721 cttcggggat ggcacgggac accctcattt acgccggtgg tgaccagacg gtacacggag
      781 aggcacataa cacccgactg gaggggggta accagtatgt acacaacggt ggcacggcaa
      841 cagagacgct gataaaccgt gatggctggc aggtgattaa ggaaggagga actgccgcgc
      901 ataccaccat caaccagaaa ggaaagctgc aggtgaatgc cggcggtaaa gcgtctgatg
      961 tcacccagaa cacgggcggt gcactggtta ccagtacagc tgcaaccgtc accggcacaa
     1021 accgcctggg agcattctcc gttgtggcgg gtaaggctga taatgtcgta ctggaaaatg
     1081 gcggacgtct ggatgtgctg agcggacaca cagccactaa tacccgtgtg gatgatggcg
     1141 gaacgctgga tatccgcaac ggtggtgccg ccaccaccgt atccatgggg aatggcggtg
     1201 tactgctggc cgattccggt gccgctgtca gtggtacccg gagcgacgga aaggcattca
     1261 gtatcggagg cggtcaggcg gatgccctga tgctggaaaa aggcagttca ttcacgctga
     1321 acgccggtga tacggccacg gataccacgg taaatggcgg actgttcacc gccaggggcg
     1381 gcacactggc gggcaccacc acgctgaata acggcgccac acttaccctt tccgggaaga
     1441 cggtgaacaa cgataccctg accatccgtg aaggcgatgc actcctgcag ggaggcgctc
     1501 ttaccggtaa cggcagcgtg gaaaaatcag gaagtggcac actcactgtc agcaacacca
     1561 cactcaccca gaaagccgtc aacctgaatg aaggcacgct gacgctgaac gacagtaccg
     1621 tcaccacgga tgtcattgct cagcgcggta cagccctgaa gctgaccggc agcacagtgc
     1681 tgaacggtgc cattgacccc acgaatgtca ccctgacatc cggtgccacc tggaatatcc
     1741 ccgataacgc cacggttcag tcggtggtgg atgacctcag ccatgccgga cagattcatt
     1801 tcacctccgc ccgcacaggg aagttcgttc cgacaactct gcaggtgaaa aacctgaacg
     1861 ggcagaatgg caccatcagc ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca
     1921 gactggtcat tgacggtggc agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg
     1981 gcaacagtgg tacggggctg gcgaccaccg gtaagggtat tcaggtggtt gaggccatta
     2041 acggtgccac cacggaggaa ggggcttttg tccaggggaa tatgttgcag gccggggcct
     2101 ttaactacac cctcaaccgg gacagtgatg agagctggta tctgcgcagt gaaaatgctt
     2161 atcgtgctga agtccccctg tatgcctcca tgctgacaca ggcaatggac tatgaccgga
     2221 ttctggcagg ctcccgcagc catcagaccg gtgtaaacgg tgaaaataac agcgtccgtc
     2281 tcagcattca gggcggccat ctcggtcacg ataacaacgg cggtattgtc cgtggtgcca
     2341 cgccggaaag cagcggcagc tatggcttcg tccgtctgga gggtgacctg ctcagaacag
     2401 aggttgccgg tatgtcactg acgacaggag tgtatggtgc tgcaggccat tcttccgttg
     2461 atgttaagga tgatgacggc tcccgtgccg gcacggtccg ggatgatgcc ggcagcctgg
     2521 gcggatacct gaatctggta cacacgtcct ccggcctgtg ggctgacatt gtggcacagg
     2581 gaacccgcca cagcatgaaa gcgtcatcgg acaataacga cttccgcgcc cggggccggg
     2641 gctggcaggg ctcactggaa accggtctgc cattcagtat cactgacaac ctgatgctgg
     2701 agccacaact gcagtatacc tggcagggac tttccctgga tgacggccag gataacgccg
     2761 gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggtttc cgtctgggca
     2821 gccacaacga tatgaccttt ggtgaaggca cctcatcccg tgacaccctg cgcgacagta
     2881 caaaacacgg tgtgagtgaa ctgcctgtga actggtgggt acagccttct gttatccgca
     2941 ccttcagctc ccggggagac atgagcatgg gtacagccgc agccggcagt aacatgacgt
     3001 tctcaccgtc ccggaatggc acgtcactgg acctgcaggc cggactggaa gcccgtgtcc
     3061 gggaaaatat caccctgggc gttcaggccg gttatgccca cagtgtcagc ggcaacagcg
     3121 ctgaaggcta taacggtcag gccacgctga atgtgacctt ctgacagaac catcgcctct
     3181 ttgtggtccc gatcatcatg atcgggaccc ggaccggcgc aacggatatt caacgccaca
     3241 ttcgctggca ttaacagtgc ggcattcgac ggacagaacg gacagtatcg ttacatggga
     3301 aatcatggtc ccatgcaact ggaagtcccc cgtgatcagt atgccggcgc tgtggaaacc
     3361 atgaagaata agatccgcga aggtaaagta cccggtgtaa ccgatcccgc agaagcgtcc
     3421 cggctgattc gtcggggaca tctgacttat acccaggccc gtaatatcac ccggttcggg
     3481 accatcgaat cggtcactta tgatattgcc gaggggtcgg ttgtcagtct ggcggccgga
     3541 gggatcagtt ttgccctgac ggcatcggtc ttctggctca gcaccggcga tcgcgatgct
     3601 gccctgcaga cagctgctgt ccaggcagga aaaaccttca cccgcacact ggctgtctac
     3661 gtcacaaccc agcaacttca ccggctcagt gttgttcagg gtatgctgaa gcatattgat
     3721 ttttcgacgg ccagcccgac tgtccggcag gcgcttcaga aggggaccgg tgcaggaaat
     3781 atcagtgccc tgaacaaagt gatgaagggt tcgctggtga catctctggc actggtagct
     3841 gtcacaaccg gccctgacat gatcaaaatg ttgcggggac ggatctccgg tgcgcagttc
     3901 atcaggaatc ttgccgtggc atcttcctgt gtggcaggtg gtgctgtcgg gtcagtggcg
     3961 ggcgggatat tgttcagtcc actgggacca tttggtgcac tgacagggcg tgtggttggc
     4021 ggtgttctgg ggggaatgat tgcctccgct gtatcaggaa aaattgccgg agcgctggtt
     4081 gaagaagatc gcgtcaaaat tctggcaatg attcaggagc agatgacatg gcttgccggc
     4141 agtttcctgc tgaccggaca tgagattgaa aatctgaacg cgaatctggc ccgtgttatc
     4201 gatcagaatg ctctggagat cattttcgcc gccggtatac aacaacgggc cgcgaccaac
     4261 atgttaatca aaccactggt ggtcagtatc atcaggcaac gccccgtcat ggaatatgat
     4321 gcatcccatc tcggcaatat ggttaaccga ctggaagaaa cattaccccc ggagttaccg
     4381 gcataacggc tattctgcgc cttttacgtt ttatcacctg atttgtcagt gtcatcgtca
     4441 tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat taccgccttc
     4501 attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta catcagtata
     4561 cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt ccggctggat
     4621 aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact caaagacaaa
     4681 tatggtcaga cgctggtgac cagaaaaatg gaaacggaag cctttggtga cagtaatgcc
     4741 acgcgaacca cagacgcatt tctggaaacg gagtgcgtgg aaaatgtcgc cacaaccgaa
     4801 atcattaaag caactgaaga aagtaacggc catcgtgtca gtctgccgtt atcggttttc
     4861 gatccccagg actaccatcc actgctgatt accgtttccg gaaaaaacgt taactgatcc
     4921 ctgaagtcag tttcatgctt cagcctctgt cacaacgcgt ggcagaggct ttttatttat
     4981 caggagaaaa cagatatgct gggttacaca gagtatgtcc tggctgaagg atctttctca
     5041 tacgggcagg cggttgctgt cataaccgct taccgcaatg tgtttatcca ggatgacccg
     5101 ggaatgcatt tccgtcgggt tatccgcaac gcggaaggtc agcgacgctg gcgctgccgg
     5161 aactctgaag ctgatgccgg aaaacagctt aatgcgtggc tcgccagtgg agggcttctc
     5221 agacaataaa cctcattgat ttcatccatc aggccgcg
//
LOCUS       DAOFSV010000220         5256 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.220, whole genome shotgun sequence.
ACCESSION   DAOFSV010000220 DAOFSV010000000
VERSION     DAOFSV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.220"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..469
                     /locus_tag="QBH27_004481"
     CDS             <1..469
                     /locus_tag="QBH27_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ7016703.1"
                     /translation="IIRAAQILQDKNIHFYLTFDGTENKYAKRIYKLASELKNVHFLG
                     YLNATEMVNFYQDSDIICFPSKLETWGLPLSEAKTYKKWIFAADLPYAHEVLYNYSKT
                     RYFPFDDEKILVRYILEYTSKNMHEDIKNSRVNFNNDALTGFEQFIEYILKGN"
     gene            471..1262
                     /locus_tag="QBH27_004482"
     CDS             471..1262
                     /locus_tag="QBH27_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ7016704.1"
                     /translation="MVYIIIVSHGHDDYIENLLLNLKLPSGRFKIIVRDNKSSMVLKK
                     TCEKNCVTYLHGGQYGFGHNNNIAVSYIINNFMIMNNDYFLFLNPDVFITSESLINYV
                     DYIISNDYKFSTLCLYRDFTKSKHDYSIRSFPTLYDFLCSFLLGVNKSKIKKENILSD
                     TVVDWCAGSFMLIHALSFLNVNGFDQKYFMYCEDIDLCMRLKLSGVDLYYTPHFDAIH
                     YAQHENRRIFTKAFRWHIRSITRYILRKPILSYKNYRKITSELVK"
     gene            1359..2765
                     /gene="gndA"
                     /locus_tag="QBH27_004483"
     CDS             1359..2765
                     /gene="gndA"
                     /locus_tag="QBH27_004483"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDQ7016705.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQRAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            3012..4178
                     /gene="ugd"
                     /locus_tag="QBH27_004484"
     CDS             3012..4178
                     /gene="ugd"
                     /locus_tag="QBH27_004484"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDQ7016706.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGTIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            4324..>5256
                     /gene="wzzB"
                     /locus_tag="QBH27_004485"
     CDS             4324..>5256
                     /gene="wzzB"
                     /locus_tag="QBH27_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDQ7016707.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQATPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSSYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGVG"
BASE COUNT         1547 a         1035 c         1138 g         1536 t
ORIGIN      
        1 tatcatacgt gctgcacaaa tattacaaga taaaaatatt catttttatc ttacttttga
       61 tggtactgaa aataagtatg caaaaagaat atataaatta gcttccgaac tgaaaaatgt
      121 acatttcctc ggttacctta atgcaaccga gatggttaac ttttatcaag attcagatat
      181 tatttgtttc ccatcgaaac tagaaacgtg gggattacca ttatcagaag ctaaaacata
      241 caaaaaatgg atatttgcgg cagacttacc ttatgctcat gaagttttat ataactattc
      301 aaaaactaga tattttccat ttgacgatga gaaaatactt gttcgctaca tattagagta
      361 cacaagtaaa aatatgcatg aagatataaa aaatagtagg gtgaatttta ataatgatgc
      421 attgactggt tttgaacagt ttattgaata tatcctcaag gggaactgac gtggtttata
      481 ttataatcgt ttcacatggc catgatgact atatagaaaa tcttttatta aatttaaagt
      541 tgccctctgg aagatttaaa ataatagttc gtgataacaa aagttcaatg gttttaaaaa
      601 aaacatgcga aaaaaattgc gtaacctatt tgcatggagg gcaatatgga tttggacata
      661 ataataacat agcagtgtca tatataatta ataacttcat gattatgaat aatgattatt
      721 ttctctttct taaccccgat gtattcataa ccagtgaaag tttgattaat tatgttgatt
      781 atataattag taatgattat aagtttagca cattatgtct ttatcgagat tttactaaaa
      841 gcaaacatga ttattcaata cggagttttc caactttata tgattttctt tgttcttttt
      901 tattgggggt gaataaaagt aaaattaaga aggaaaatat actttctgat actgtagttg
      961 attggtgtgc tggctcattt atgcttattc atgctttaag tttcttaaat gtgaatggtt
     1021 ttgatcaaaa atattttatg tattgtgaag atattgacct ttgtatgcgt ttaaaattaa
     1081 gtggagtaga tctttactat actccccatt ttgatgctat tcattatgcg cagcatgaaa
     1141 atagaagaat atttactaaa gcatttcgat ggcatataag gagtattacg cgctacatat
     1201 tacggaaacc aattctttct tataaaaact atagaaaaat tacatccgaa ctggtaaagt
     1261 gattaaataa agtcctaatt ttactactga aatggtatct ttatttaaag atctttgctt
     1321 tatataccaa ttttttaacg ccctgacagg agtaaacaat gtcaaagcaa cagatcggcg
     1381 tcgtcggtat ggcagtgatg gggcgcaacc ttgcgctcaa catcgaaagc cgtggttata
     1441 ccgtctctat tttcaaccgt tcccgtgaaa aaacggaaga agtgattgcc gaaaatccag
     1501 gcaaaaaact ggttccttac tatacggtga aagagtttgt tgaatctctg gaaacgcctc
     1561 gtcgcatcct gttaatggtg aaagcaggtg caggcacgga tgctgctatt gattccctta
     1621 agccgtacct cgataaaggt gacatcatca ttgatggtgg taacaccttc ttccaggaca
     1681 ctattcgtcg taatcgtgag ctttctgcag aaggctttaa cttcatcggt actggtgttt
     1741 ctggcggtga agaaggcgcg ctgaaaggac cttccattat gcctggtggc cagaaagaag
     1801 cctatgaact ggttgcgccg atcctgacca aaatcgccgc agtggctgaa gacggggagc
     1861 catgcgttac ctatattggt gccgatggcg caggccacta tgtgaagatg gttcacaacg
     1921 gtattgaata cggtgatatg cagctgattg ctgaagccta ttctctgctt aaaggtggcc
     1981 tgaacctcac caacgaagaa ctggcgcaga cctttaccga gtggaataac ggtgaactga
     2041 gcagctacct gattgacatt accaaagata tcttcaccaa aaaagatgaa gacggtaact
     2101 acctggttga tgtgattctg gatgaagctg caaacaaagg tacgggtaaa tggaccagcc
     2161 agagcgcgct ggatctcggc gaaccgctgt cgctgattac cgagtctgtg tttgcacgtt
     2221 atatctcttc tctgaaagat cagcgcgttg ccgcgtctaa agttctctct ggcccgcaag
     2281 cgcagcgagc aggcgacaaa gctgagttta tcgagaaggt tcgtcgtgcg ctgtatctgg
     2341 gcaaaatcgt ttcttacgct cagggcttct ctcagttgcg tgctgcgtct gaagaataca
     2401 actgggatct gaactacggc gaaatcgcga agattttccg tgctggctgc atcatccgtg
     2461 cgcagttcct gcagaaaatc accgatgcat acgccgaaaa tccgcagatc gctaacctgc
     2521 tgctggctcc gtactttaag caaatcgccg atgactatca gcaggcgctg cgcgatgtcg
     2581 ttgcttatgc agtacagaac ggtatcccgg ttccgacctt cgccgctgcg gttgcctatt
     2641 atgacagcta ccgtgccgct gttctgcctg caaacctgat ccaggcacag cgtgactatt
     2701 tcggtgcaca tacttataag cgcatcgata aagaaggtgt gttccatacc gaatggctgg
     2761 attaatctga cttaactaac aataaattga ggcccggcgt atattgtgcc gggctttttt
     2821 tgccatttat cttatagact aaatttcact gcttaatatt aacttaataa atatcagcta
     2881 ttcttataaa gaaattctga attgtttttc gttgcgttga ccagctatcg acgtagcgtt
     2941 aaaactttta actcttgtca ggatgctaaa aacatcatga ttcacagtta agttaattct
     3001 gagagcatga aatgaaaatt accatttccg gtactggcta tgtaggcttg tcaaacgggc
     3061 ttctaatcgc acaaaatcat gaggttgtgg cattagatat tttaccgtca cgcgttgcta
     3121 tgctgaatga tcggatatct cctattgttg ataaggaaat tcagcagttt ttgcaatcag
     3181 ataaaataca ctttaatgcc acattagata aaaatgaagc ctaccgggat gctgattatg
     3241 tcatcatcgc cactccaacc gactatgatc ctaaaactaa ttatttcaat acatccagtg
     3301 tagaatcagt aattaaagac gtagttgaga taaaccctta tgcggttatg gtcatcaaat
     3361 caacggttcc cgttggtttt accgcagcga tgcataaaaa atatcgtact gaaaatatta
     3421 tattctcccc ggaatttctc cgtgagggta aagcccttta cgataacctt catccgtcac
     3481 gtattgtcat cggtgagcgt tcagaacgcg cagaacgttt tgctgcgttg ttacaggaag
     3541 gcacgattaa gcaaaatatc ccgaccctgt ttaccgactc cactgaagca gaagcgatta
     3601 aacttttcgc taatacctat ctggcgatgc gcgtggcgta ctttaacgaa ctggatagct
     3661 atgcagaaag tttgggtctg aatacgcgtc agattatcga aggtgtttgt ctcgacccgc
     3721 gtatcggcaa ccattacaac aatccgtcgt ttggttatgg tggttattgt ttgccgaaag
     3781 ataccaaaca gttattggcg aattatcagt ctgtgccgaa taacctgatt tcggcaattg
     3841 tcgatgctaa ccgcacgcgt aaagatttta ttgccgatgc cattttgtca cgcaagccgc
     3901 aagtggtggg tatttatcgt ctgattatga agagcggttc agacaacttc cgcgcgtctt
     3961 ccattcaggg gattatgaaa cgtatcaagg cgaaaggcgt tgaagtgatc atttacgaac
     4021 cggtgatgaa agaagactca ttcttcaact ctcgcctgga acgagatctc gccactttca
     4081 aacaacaagc tgatgtcatt atctccaatc gtatggcaga agagcttaag gatgtggcag
     4141 ataaggtata cacccgcgat ctctttggta gcgattaaca tcttgttatc agggctattt
     4201 acgccctgat tgtcttttgt tacttcctca acaattcatt atttttatca cttatcctat
     4261 agcattcacg aggattatcg ctaaactatg cggacttgga aatttccgtc agttagggta
     4321 atgatgagag tagaaaataa taatgtttct gggcaaaacc atgacccgga acagattgat
     4381 ttgattgatt tactagtgca gttgtggcgt ggcaagatga caatcatcat ttccgtcatt
     4441 gtggctattg ccctggctat tggttatttg gcagtagcga aggagaaatg gacgtcaaca
     4501 gcaattatca ctcagcccga cgtggggcaa attgctggct ataacaatgc catgaatgtt
     4561 atctatggtc aggctacacc gaaagtatcg gatttgcagg agacgttaat tggtcgcttc
     4621 agttctgcct tctctgcatt agcagaaacg ctggataatc aggaagagcc agaaaaactt
     4681 accatcgaac cttctgttaa gaaccagcaa ttaccattga ctgtttctta tgttgggcaa
     4741 actgcagagg gcgcacaaat gaagttggcc caatacattc agcaagttga tgataaagtg
     4801 aatcaagagc tagaaaagga tctcaaggac aacattgctc tgggacggaa aaacttgcag
     4861 gactctttaa gaacccagga agtggtcgcg caggaacaga aagatctgcg tatccgtcag
     4921 attcaggaag cgttgcagta tgcgaatcag gcgcaggtga caaagccgca gattcaacag
     4981 acgcaggacg taacccagga tactatgttc cttttgggga gcgaagcgct ggagtcgatg
     5041 attaagcacg aggcgacccg tccgttggtg ttctcatcaa gctactatca gactcgtcaa
     5101 aacctgctgg atatcgacaa tcttgacgtt gataaacttg atattcatgc ttaccgctat
     5161 gtaatgaaac cgacgttacc tattcgtcgc gatagcccga aaaaggcaat taccttgatt
     5221 ctggcggtgc tgctgggcgg catggttggc gtgggg
//
LOCUS       DAOFSV010000221         5244 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.221, whole genome shotgun sequence.
ACCESSION   DAOFSV010000221 DAOFSV010000000
VERSION     DAOFSV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.221"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..696)
                     /gene="bamD"
                     /locus_tag="QBH27_004486"
     CDS             complement(<1..696)
                     /gene="bamD"
                     /locus_tag="QBH27_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDQ7016708.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAE"
     gene            831..1811
                     /gene="rluD"
                     /locus_tag="QBH27_004487"
     CDS             831..1811
                     /gene="rluD"
                     /locus_tag="QBH27_004487"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDQ7016709.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            1808..2539
                     /gene="pgeF"
                     /locus_tag="QBH27_004488"
     CDS             1808..2539
                     /gene="pgeF"
                     /locus_tag="QBH27_004488"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDQ7016710.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVGENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2669..5242
                     /gene="clpB"
                     /locus_tag="QBH27_004489"
     CDS             2669..5242
                     /gene="clpB"
                     /locus_tag="QBH27_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDQ7016711.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1298 a         1259 c         1432 g         1255 t
ORIGIN      
        1 ttcagcttgc gcattcatct gcatctgacg gtatgcattt tccatcagcg gcagcgcatc
       61 acgcgtagcc tgggtatccg gatagtcgcg caacatgcct tctacgcggt taacgacagc
      121 aacccatgca ccacgttctg tataatactc ggcaacggag tattcatatt tcgccagacg
      181 atctttcagg aataccagac gtttggtggc atcggtggtg tactgactgt tcggatagcc
      241 gcgcaccagt ttggaaaagt cactaaacgc agctcgtgca tgttgaggat cgcgatcgct
      301 acggtcgacg ccaaagaacc cttgcagtgc actgtcatcg agcgccatat tggtcaggcc
      361 acgcatgtac atgacataat cgatattcgg atgggtcggg ttaaggcgaa taaaacgatc
      421 gatggcagcc tgtgctaacg gcaaatcggc gtttttatag taggcgtaga tgagatccag
      481 ctgcacctgc tgcgaatacg gaccaaacgg atagcgatta tctaacgctt ccagttgcgt
      541 tattgcctgt ctccagttac cgtcctgcag cttttgttgt gcagtcgcgt aaatttcatt
      601 tggcggatta tcaggtactt cttcctttga ccccgagcaa cccgccaaaa acaggcttag
      661 tgtggcggct gccaccagat atttcatgcg cgtcatgacg ttttgacttt cctcaaaatg
      721 taatacggga gattctctgt tcctgctccc ggttaagacc agctacaata gcacactata
      781 ttaaacggca aagccgtaaa accccaacga taaacgaaga agcagtatat atggcacaac
      841 gagtacagct cactgcaacg gtgtccgaaa accaactcgg tcaacgctta gatcaggctt
      901 tggccgaaat gttcccggat tattcacgtt cgcggataaa agaatggatc ctcgaccagc
      961 gtgtgctggt taacggcaaa gtgtgtgata agccgaaaga aaaagtattg ggtggcgagc
     1021 aggttgccat caacgctgag attgaagaag aagcgcgttt tgaaccgcag gatatcccgc
     1081 tggatatcgt ctatgaagat gaagatatca tcgttattaa taaaccgcgc gacctggtgg
     1141 tacatcctgg cgcgggtaac ccggatggca cggtactgaa tgcattgctt cattattatc
     1201 cgcccattgc cgatgtaccg cgtgcgggca ttgtccatcg tctggataaa gacaccactg
     1261 gcctgatggt tgtggcaaaa accgttccgg ctcagacgcg tttagtcgaa tctttgcaac
     1321 ggcgtgaaat tacacgtgag tatgaagcgg tggcaattgg tcatatgacc gctggcggta
     1381 cagtagatga accaatcagc cgccacccga ccaaacgtac tcacatggcg gtacatccca
     1441 tgggtaaacc ggcggtcact cactatcgca tcatggaaca cttccgtgtg cacacgcgtc
     1501 tgcggttgcg tctggaaacc ggacgtacgc accagatccg cgtgcatatg gcccatatca
     1561 ctcatccgct ggtgggcgat ccggtttatg gtggacgtcc gcgtccgcca aaaggtgctt
     1621 cggaagcgtt tatctccacg ctgcgtaagt ttgaccgtca ggcgcttcat gcaaccatgc
     1681 tgcgtcttta tcacccgatc tccggcatcg aaatggaatg gcatgcgcct attccacaag
     1741 atatggtgga gctgattgag gagatgcgcg ccgatttcga agagcataag gatgaagtgg
     1801 actggttatg agtaagctga ttgtcccgca gtggccgctg ccaaaaggtg ttgcggcttg
     1861 tagctctacc cgtatcggcg gtgtgagctt acccccgtat gactcgctca acctcggtgc
     1921 ccattgtggc gataacccgg atcacgttgg ggagaatcgc aagcgacttt ttgctgcggg
     1981 caatttgcct tctaaaccgg tctggcttga gcaggtacac ggcaaagacg tgcttaagct
     2041 tattggcgaa ccttatgcct caaaacgagc agatgcctct tatagcaata cgcctggcac
     2101 ggtttgcgca gtgatgactg ccgactgcct gcccgtgctg ttttgcaatc gtgcgggaac
     2161 ggaggtcgcc gccgctcatg ctggctggcg tggactgtgc gcaggcgtgt tggaagagac
     2221 ggtttcctgt tttgctgata atccggaaaa tattctcgcc tggttagggc cggcaattgg
     2281 gccacgtgcg ttcgaagtgg gggcggaggt tcgcgaggcg tttatggcag tagacgccaa
     2341 agcgagtaca gctttcattc agcatggtga taagtatctg gcggatattt atcagcttgc
     2401 ccggcagcgt ctggcgagcg ttggtgttga acaaattttc ggcggcgacc gttgtacata
     2461 tacggaaaat gagactttct tctcttatcg tcgcgacaag accaccggtc gtatggcaag
     2521 tttcatttgg ctgatataac ctaaagaatc aagacgatcc ggtacgcgtg attttctttt
     2581 cacattaatc tggtcaataa ccttgaataa ttgagggatg acctcattta atctccagta
     2641 gcaactttga tccgttatgg gaggagttat gcgtctggat cgtcttacta ataaattcca
     2701 gcttgctctt gccgatgccc aatcacttgc actcgggcac gacaaccaat tcatcgaacc
     2761 acttcattta atgagcgccc tgctgaatca ggaagggggt tcggttagtc ctttattaac
     2821 atccgctggc attaatgctg gccagttgcg cacagatatc aatcaggcat taaatcgttt
     2881 accacaggtt gaaggtactg gtggtgatgt ccagccatca caggatctgg tgcgcgttct
     2941 taatctttgc gacaagctgg cgcaaaaacg tggtgataac tttatctcgt cagaactgtt
     3001 cgttctggcg gcacttgagt ctcgcggcac gctggccgac atcctgaaag cagcaggggc
     3061 gaccaccgcc aacattactc aagcgattga acaaatgcgt ggaggtgaaa gcgtgaacga
     3121 tcaaggtgct gaagaccaac gtcaggcttt gaaaaaatat accatcgacc ttaccgaacg
     3181 agccgaacaa ggcaaactcg atccggtgat tggtcgtgat gaagaaattc gccgtaccat
     3241 tcaggtgctg caacgtcgta ctaaaaataa cccggtattg attggtgaac ccggcgtcgg
     3301 taaaactgcc atcgttgaag gtctggcgca gcgtattatc aacggcgaag tgcccgaagg
     3361 gttgaaaggc cgccgcgtac tggcgctgga tatgggcgcg ctggtggctg gggcgaaata
     3421 tcgcggtgag tttgaagaac gtttaaaagg cgtacttaac gatcttgcta aacaggaagg
     3481 caacgtcatc ctgtttatcg acgaattaca taccatggtc ggcgcgggta aagccgatgg
     3541 cgcaatggac gccggaaaca tgctgaaacc agcgttggcg cgtggtgaat tgcactgcgt
     3601 aggtgccacg acgcttgacg aatatcgcca gtacatcgaa aaagatgctg cgctggaacg
     3661 tcgtttccag aaagtgtttg ttgccgagcc ttctgttgaa gataccattg ctattctgcg
     3721 aggcctgaaa gaacgttacg agctgcacca ccatgtgcaa attactgacc cggcaattgt
     3781 tgcagcagcg acgttgtctc atcgctacat tgctgaccgt cagctgccgg ataaagccat
     3841 cgacctgatc gatgaagcag catccagcat tcgtatgcag attgactcaa aaccagaaga
     3901 actcgaccga ctcgatcgcc gtatcatcca gctcaaactg gaacaacagg cgttaatgaa
     3961 agagtctgat gaagccagta aaaaacgcct ggatatgctc aacgaagaac tgagcgacaa
     4021 agaacgtcag tactccgagt tagaagaaga gtggaaagca gagaaagcct cgctttctgg
     4081 tacgcagacc attaaagcgg aactggaaca ggcgaaaatt gctattgaac aggctcgccg
     4141 tgtgggtgac ctggcgcgga tgtctgaact gcaatacggc aaaatcccgg aactggaaaa
     4201 gcaactggaa gccgcaacgc agctcgaagg caaaactatg cgtctgttgc gtaataaagt
     4261 gaccgatgcc gaaattgctg aagtgctggc gcgttggacg gggattccgg tttctcgcat
     4321 gatggaaagc gagcgcgaaa aactgctgcg tatggagcaa gaactgcacc atcgcgttat
     4381 tggtcagaac gaagcggttg atgcggtatc taacgctatt cgtcgtagcc gtgcggggct
     4441 ggcggatcca aatcgcccga ttggttcatt cctgttcctc ggcccaaccg gtgtggggaa
     4501 aactgagctt tgtaaggcgc tagcgaactt tatgtttgat agcgacgagg cgatggtccg
     4561 tatcgatatg tccgagttta tggagaaaca ctcggtgtct cgtctggttg gtgcgcctcc
     4621 gggatatgtc ggttatgaag aaggtggcta cctgaccgaa gcggtgcgtc gtcgtccgta
     4681 ttccgtcatt ctgctggatg aagtggaaaa agcgcatccg gatgtcttca acattctgtt
     4741 gcaggtactg gatgatgggc gtctgactga cgggcagggg agaacggtcg acttccgtaa
     4801 tacggttgtc attatgacct ctaacctcgg ttccgatttg attcaggaac gcttcggtga
     4861 actggattat gcgcacatga aagagctggt gctcggtgtg gttagccata acttccgtcc
     4921 ggaattcatt aaccgtatcg atgaagtggt ggtcttccat ccgctgggtg aacagcacat
     4981 tgcgtcgatt gcgcagattc agttgaaacg tctgtataaa cgtctggaag aacgtggtta
     5041 tgaaatccac atttctgacg aggcgctgaa actgctgagc gagaacggtt acgatccggt
     5101 ctatggcgca cgtcctctga aacgtgcaat tcagcagcag attgaaaacc cgctggcaca
     5161 gcaaatactg tctggtgaat tggttccggg taaagtgatt cgcctggaag ttaatgaaga
     5221 ccggattgtc gccgtccagt aaat
//
LOCUS       DAOFSV010000222         5238 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.222, whole genome shotgun sequence.
ACCESSION   DAOFSV010000222 DAOFSV010000000
VERSION     DAOFSV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.222"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(98..364)
                     /gene="minE"
                     /locus_tag="QBH27_004490"
     CDS             complement(98..364)
                     /gene="minE"
                     /locus_tag="QBH27_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDQ7016712.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(368..1180)
                     /gene="minD"
                     /locus_tag="QBH27_004491"
     CDS             complement(368..1180)
                     /gene="minD"
                     /locus_tag="QBH27_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDQ7016713.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1204..1899)
                     /gene="minC"
                     /locus_tag="QBH27_004492"
     CDS             complement(1204..1899)
                     /gene="minC"
                     /locus_tag="QBH27_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDQ7016714.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKSPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2419..2787
                     /locus_tag="QBH27_004493"
     CDS             2419..2787
                     /locus_tag="QBH27_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016715.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(2890..3291)
                     /locus_tag="QBH27_004494"
     CDS             complement(2890..3291)
                     /locus_tag="QBH27_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDQ7016716.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3532..3825
                     /locus_tag="QBH27_004495"
     CDS             3532..3825
                     /locus_tag="QBH27_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDQ7016717.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            3897..4556
                     /locus_tag="QBH27_004496"
     CDS             3897..4556
                     /locus_tag="QBH27_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDQ7016718.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKVGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            4648..5094
                     /locus_tag="QBH27_004497"
     CDS             4648..5094
                     /locus_tag="QBH27_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDQ7016719.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1331 a         1181 c         1260 g         1466 t
ORIGIN      
        1 gaatactcgc cgctttacca caacgtggat gagagggatg aaaaactcaa ggcagagatg
       61 actctgcctt gaagataaat gcgcttttac agggggctta tttcagctct tctgcttccg
      121 gtaaggtcac gttcagctca agaatagaaa tatcgccatc tttttgctca agctgtacgg
      181 ttaccatctc aggatcaatt tgtacgtatt tacaaatgac ctcaagaata tctttacgca
      241 actgcggcag ataatgcggt tctgcatcgc tgcgacggcg ttcagcaaca ataatctgca
      301 accgttcttt tgcaatgttg gctgtgtttt tcttccgcga gagaaagaaa tcgagtaatg
      361 ccataactta tcctccgaac aagcgtttga ggaagccttt cttctcttct tcaatgaagc
      421 ggaaaggacg ttcttctccc aacagacgtt ctacggtatc tgcgtaggct ttacccgcat
      481 cggcgttaat gtcgagaatg accggttcac cctggttaga ggcgcgcaat actgattgat
      541 cctctgggat cacgccgacg agtttgatgc gcaggatctc cagcacatct tccatgctca
      601 gcatgtcacc tctgcttacg cggcctgggt tatagcgcgt taacagcagg tgctctttaa
      661 tcggctcttc gccattttct gcgcggcgtg atttcgacgc cagaatgcct aaaatacggt
      721 cagagtcgcg tactgaggag acttccgggt tggtggtaat aatggcttcg tctgcaaaat
      781 agagtgccat taacgcaccg gtttcaatcc ctgccgggga gtcacaaacg ataaattcaa
      841 aatccatcgc tttcagatca tcaagaactt tggcgacccc ttcacgggtg agggcatctt
      901 tatcgcgagt ttgcgatgcc ggaagaatat agagattttc agtacgttta tctttaatta
      961 acgcctggtt tagcgttgca tcgccctgaa tgacgttgac gaaatcgtaa acgacccggc
     1021 gttcacaacc cataatcagg tcgagattac gcaggccgat atcaaaatct atcacgacag
     1081 ttttctttcc cttctgggcc aaaccagtgg cgatggccgc gctggaggtt gtcttaccaa
     1141 cacccccttt gcccgaagta acaacaataa tgcgtgccat agaaattcct tgttaaaaag
     1201 ggatcaattt aacggttgaa cggtcaaagc gttttcgact aactgcagtc gtgccgcttt
     1261 gccataaaat tctgctggga tttgatcact cagccagtat tcacctgcga tggacaccag
     1321 ttccgccatc aggttcgtac aaaatatttg cgtttcccgg tcaccacttg cccctgccag
     1381 cgcacgaccg cgcatcatgc catagacatg aatgttacca tcggcaatca attcggcccc
     1441 tgcgctaacg tggcttgtaa caatcagatc acattgtgga gcataaatac gctgaccgga
     1501 acgcactggg gtatcaatta aacgcgtttt tgtgaccggc gttgtatttt gcgctggagc
     1561 ctgcggtgcg ggggctggac gcggcgattt ttcctttcct tccgtcagga taggcagccc
     1621 catcttttca atttcggctt taagttgcgc atctttgcag ccacttacgc caataacccg
     1681 caaaccagtt gccgaaaccg ccttatgcat cgccgaccag tttaccgggt cttccagtgc
     1741 actgacgttg agtacaacgg gggcatgttt taaaaatgcg ggggcctgag cgattttgtc
     1801 ttccagcgcc tgatggataa ccttaggttc tgcctcatgc agatgaacca cagataaagt
     1861 gaagctactg cctttaagct cgattggcgt gtttgacatc ctggccttac tcaattagct
     1921 attaatcatc gccagcgcgc gatgatgttc cgaagactat aaggcatgtt atagtctgga
     1981 ttatattgag gcaagtcacc ctcccattta ttcagagtaa aagtctattc tgtgataaat
     2041 ggcgttgatt catagcttaa aaaataccct tgtcaatcaa cccattgccg tcgtactttt
     2101 gattgttctt atttacgctt cctttttccg caccctaatc gtgacagcat cttcagacct
     2161 gtttaaatat ccagtattga ggcgagtgta ttttttcacc acgccaggct ttcttaactt
     2221 tccatttttt gcagcatcat taaagaacag atacccgtta tcttaatatc cgccgcagaa
     2281 ttgctctatc atagaaaccg cagaagacgt ctgatacttc tgtcaggtca tcgtaactcg
     2341 tccaggtcag cggttttgat tacggattga tgatgaaatg agcataagtg ttgtacggtt
     2401 ggattatcag gatgtgctat gaatatgatg cgtatttttt atatcggatt gtcaggtgtg
     2461 gggatgatgt tctcatcaat ggcgtctgga aatgacgctg gcgggcttca atctcctgcg
     2521 tgtggcgttg tttgcgaccc gtatatttgt gtgagttcag atggcatttc cccagagtta
     2581 acaaggaaat atctcggcga aaaagccgct gaaaacttac aatcattaca aggctacgat
     2641 cccagcgaat ttacattctc taacggtgta ttttgcgatg ttaaagaaaa attatgtcgt
     2701 gatgatcgtt attttggtgt ggatggtaag cggagtggaa aaatcaatca aaccaccaca
     2761 aagatgttat ttatgtgtcg tgagtgatgt cttgtttaca ttatgttcta ttcgtcatta
     2821 acctcttaag cgacagagag gagtgtggcg ttaacgttta tgacggggag agtccccgtc
     2881 ggttgacatt tacttaattt gaatatcgac gttatatttt ttagttttgt ttttacgggc
     2941 gtcgtttcgg gtttgcagca ctcgcagctc atatttgcca gaagcaggga gtgagtattg
     3001 tccgtgacta tcgagttctg gggaatatct ggataaatca acagaatcat cgattcctgg
     3061 cccgaagagg taagtatcag cgccttcatt agagatactc acatgcactt tctgaccttt
     3121 tttggcatag aaggtatatg tatcgtaatc gtatcccttt atttcgccgg agtactgagc
     3181 gctactatgg ccttttctga actcaacatt gacattctta cccgcagcaa agctcgtgga
     3241 agttagcagt gcaagcaaca atacagcctt actgatgccc ttaattttca ttggtagtct
     3301 cctgttcaga atgagtacga tgaactgtta taatataaca atccttcacg ggagaattcc
     3361 agtggctttc ctgtgttcgg ttaaatcctg gcgcggcatt tttgccgggg gattacttat
     3421 aaagggggca tggactgaac aaaccgtaga aatctacagc tggctggctt gtggcgcggg
     3481 tttcattgat gttagcttat gccaaaacct ggcatcttaa agagtaaaag tatgttttgt
     3541 gtgatttatc gaagcagcaa acgtgaccag acctatttat atgtcgaaaa aaaagacgat
     3601 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
     3661 cttccgctgg atgggcggaa gaaactggtc aatgccgata ttgaaaaggt taaacaggca
     3721 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
     3781 catctttccg tcatggggca gaaaacagac gacactaaca aataaccgat atccggcggt
     3841 ggcattatct ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt
     3901 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     3961 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtaccc gaagagccag
     4021 tgctgtttat taaaccagaa acggcactgt gcgatctgcg gcagccattg gcgatcccat
     4081 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggc gcgacgctgc
     4141 gtcaggctac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgctcgatc
     4201 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcca
     4261 aagcgtttga taactcttgt ccgctttccg ggtttattcc cgcggcggaa tttaccggcg
     4321 acccgcaaaa tacaacgctg ggcctgagcg taaacggcga gcaacgccag caaggtacga
     4381 ctgcggacat gatccataaa atcgttccgc tgatcgctta tatgagcaag ttttttaccc
     4441 tcaaggtcgg tgacgttgtg ctgacaggca cgcctgatgg tgtcggcccg ttgcaaagcg
     4501 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact
     4561 ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctgt gccagactgg ttataaggtg
     4621 cgttttaacg taatggcgga acacctgatg agcgatgtac ctttctggca aagtaaaacc
     4681 ctggacgaaa tgagcgatgc agaatgggag tcgttgtgtg atggttgcgg tcagtgttgc
     4741 ctgcataaac tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc
     4801 cagctcaata ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc
     4861 gactgcatta aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc
     4921 gcttatcgtt tgctggcgga aggtaaaggt ttacctgcgt ggcatccgct acttactggt
     4981 tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca
     5041 gaagtgattg actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt
     5101 taatagatgt tgatgggggc tttcttaata tacgtctgga tgctcgccat ttcgtagccc
     5161 tgtttttgaa agcgctctgt cataatgaga gttacccgat actttattat caacaggtaa
     5221 ctctcaatct ttaaaaaa
//
LOCUS       DAOFSV010000223         5238 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.223, whole genome shotgun sequence.
ACCESSION   DAOFSV010000223 DAOFSV010000000
VERSION     DAOFSV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.223"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1273)
                     /gene="gltA"
                     /locus_tag="QBH27_004498"
     CDS             complement(<1..1273)
                     /gene="gltA"
                     /locus_tag="QBH27_004498"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDQ7016720.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYAQRDFKSD"
     gene            complement(1479..1586)
                     /locus_tag="QBH27_004499"
     CDS             complement(1479..1586)
                     /locus_tag="QBH27_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016721.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            1967..2371
                     /gene="sdhC"
                     /locus_tag="QBH27_004500"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             1967..2371
                     /gene="sdhC"
                     /locus_tag="QBH27_004500"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDQ7016722.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2365..2712
                     /gene="sdhD"
                     /locus_tag="QBH27_004501"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2365..2712
                     /gene="sdhD"
                     /locus_tag="QBH27_004501"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDQ7016723.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2712..4478
                     /gene="sdhA"
                     /locus_tag="QBH27_004502"
     CDS             2712..4478
                     /gene="sdhA"
                     /locus_tag="QBH27_004502"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDQ7016724.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4494..5210
                     /gene="sdhB"
                     /locus_tag="QBH27_004503"
     CDS             4494..5210
                     /gene="sdhB"
                     /locus_tag="QBH27_004503"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDQ7016725.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
BASE COUNT         1232 a         1286 c         1413 g         1307 t
ORIGIN      
        1 tatccgactt aaaatcacgc tgcgcgtagc cggtatacag ctgacgcgga cgggcaatct
       61 tcataccgtc actgtgcatt tcgctccagt gggcgatcca gccaaccgta cgtgccatag
      121 cgaagatcac ggtgaacata gaagacggaa tacccatcgc tttcaggatg ataccagagt
      181 agaaatcgac gttcgggtac agttttttct cgataaagta cgggtcgttc agcgcgatgt
      241 tttccagctc catagccact tccagcaggt catctttggt gcccagctct ttcagcactt
      301 catggcaggt ttcacgcatt acggtggcgc gcgggtcgta gtttttgtac acgcgatgac
      361 cgaagcccat caggcggaaa gaatcatttt tgtctttcgc acgacgaaca aattccggaa
      421 tgtgtttaac ggagctgatt tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt
      481 gcgcaggtcc ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg
      541 aagagccagc ggtacgcacg gtggaggtgg aggcgttctg ttcatggtca gcgtgcagga
      601 tcagaatacg gtccatagca cgttccagaa tcggattaac ttcatacggt tcgcacggtg
      661 tggagaacat catattcagg aagttaccgg cgtaggagag atcgttgcgc gggtaaacaa
      721 atggctgacc aatggaatac ttgtaacaca tcgcggccat ggtcggcatt tttgacagca
      781 ggcggaacgc ggcaatttca cgatgacgag gattgttaac atccagcgag tcgtgataga
      841 acgctgccag cgcgccggta ataccacaca tgactgccat tggatgcgag tcgcgacgga
      901 aagcatggaa cagacgggta atctgctcgt ggatcatggt atgacgggtc accgtagttt
      961 taaattcgtc atactgttcc tgagtcggtt tttcaccatt cagcaggatg taacaaactt
     1021 ccaggtagtt agaatcggtc gccagctgat cgatcgggaa accgcggtgc agcaaaatac
     1081 cttcatcacc atcaataaaa gtaattttag attcgcagga tgcggttgaa gtgaagcctg
     1141 ggtcaaaggt gaacacacct tttgaaccga gagtacggat atcaataaca tcttgaccca
     1201 gcgtgccttt cagcacatcc agttcaacag ctgtgtcccc gttgagggtg agttttgctt
     1261 ttgtatcagc catttaaggt ctccttagcg ccttattgcg taagactgcc ggaacttaaa
     1321 tttgccttcg cacatcaacc tggctttacc cgtttgttat ttggctcgcc gctctgtgaa
     1381 agaggggaaa acctgggtac agagctctgg gcgcttgcag gtaaaggatc tattgatgac
     1441 gaataaatgg cgaatcaagt acttagcaat ccgaattatt aaacttgtct accactaata
     1501 actgtcccga atgaattggt caatacttcc acactgttac ataagttaat cttaggtgaa
     1561 ataccgactt cataactttt acgcattata tgcttttcct agtaatgttt gtaacaactt
     1621 tgttgaatga ttgtcaaatt agatgattaa aaattaaata aatgttgtta tcgtgacgca
     1681 gatcactgtt cgggataaaa cccgacaaac tatatgtagg ttaattgtaa tgattttgtg
     1741 aacagcctat actgccgcca ggtctccgga acaccctgca atcccgagcc acccagcgtt
     1801 gtaacgtgtc gttttcgcat ctggaagcag tgttttgcat gacgcgcagt tatagaaagg
     1861 acgctgtctg acccgcaagc agaccggagg aaggaaatcc cgacgtctcc tggtaacaga
     1921 aagttaacct ctgtgcccgt agtccccagg gaataataag aacagcatgt gggcgttatt
     1981 catgataaga aatgtgaaaa aacaaagacc tgttaatctg gacctacaga ccatccggtt
     2041 ccccgtcacg gcgatagcgt ccattctcca tcgcgtttcc ggtgtgatca cctttgttgc
     2101 agtgggcatc ctgctgtggc ttctgggtac cagcctctct tcccctgaag gtttcgagca
     2161 agcttccgcg attatgggca gcttcttcgt caaatttatc atgtggggca tccttaccgc
     2221 tctggcatat cacgtcgtcg taggtattcg ccacatgatg atggattttg gctatctgga
     2281 agaaacattc gaagcgggta aacgctccgc caaaatctcc tttgttatta ctgtcgtgct
     2341 ttcacttctc gcaggagtcc tcgtatggta agcaacgcct ccgcattagg acgcaatggc
     2401 gtacatgatt tcatcctcgt tcgtgctacc gctatcgtcc tgacgctcta catcatttat
     2461 atggtcggtt ttttcgctac cagtggcgag ctgacatatg aagtctggat tggtttcttc
     2521 gcctctgcgt tcaccaaagt gttcaccctg ctggcgctgt tttctatctt gatccatgcc
     2581 tggatcggca tgtggcaggt gttgaccgac tacgttaaac cgctggcctt gcgcctgatg
     2641 ctgcaactgg tgattgtcgt tgcactggtg gtttacgtga tttatggatt cgttgtggtg
     2701 tggggtgtgt gatgaaattg ccagtcagag aatttgatgc agttgtgatt ggtgctggcg
     2761 gcgcaggtat gcgcgcggcg ctgcaaattt cccagagtgg ccagacctgt gcgctgctct
     2821 ctaaggtctt cccgacccgt tcccataccg tttctgcgca aggtggtatt accgttgcgc
     2881 tgggtaatac ccatgaagat aactgggaat ggcatatgta cgacaccgta aaagggtcgg
     2941 actatatcgg tgaccaggac gcgattgaat atatgtgtaa aaccgggccg gaagcgattc
     3001 tggaactgga acatatgggc ctgccgttct cgcgtcttga tgatggtcgt atctatcaac
     3061 gtccgtttgg cggtcagtcg aaaaacttcg gcggcgagca ggcggcacgt actgcggcgg
     3121 cagctgaccg taccggtcac gcactgttgc acacgcttta tcagcagaac ctgaaaaacc
     3181 acaccaccat tttctccgag tggtatgcgc tggatctggt gaaaaaccag gatggcgcag
     3241 tggtcggttg taccgcactg tgcatcgaaa ctggtgaagt ggtttacttt aaagctcgcg
     3301 cgacagtgct ggcgactggc ggggcagggc gtatttatca gtccaccacc aacgcccaca
     3361 ttaacactgg cgacggtgtc ggcatggcta tccgtgcagg cgtaccggtg caggatatgg
     3421 aaatgtggca gttccacccg accggtattg ccggtgcggg cgtactggtc accgaagggt
     3481 gccgtggtga aggcggttat ctgctgaaca aacatggcga acgctttatg gagcgttatg
     3541 cgccgaacgc caaagacctg gcgggccgtg acgtggtggc gcgttccatc atgatcgaaa
     3601 tccgtgaagg tcgcggctgt gatggtccgt gggggccaca cgcaaaactg aaacttgacc
     3661 atctggggaa agaagttctc gaatcccgtc tgccgggtat tctggaactc tcccgtacct
     3721 tcgctcacgt cgatccggtg aaagagccga ttccggttat cccaacctgt cactacatga
     3781 tgggcggtat tccgaccaaa gtgaccggtc aggcgctgac ggtgaatgag aaaggcgaag
     3841 atgtggttgt tccggggctg tttgccgttg gtgaaatcgc ttgtgtatcg gtacacggcg
     3901 ctaaccgtct gggcggcaac tcgctgctgg acctggtggt atttggtcgt gcggcaggtc
     3961 tgcatctgca agagtctatc gccgagcagg gcgcactgcg cgatgccagc gagtctgatg
     4021 tagaagcgtc tctggatcgc ctgaaccgct ggaacaataa ccgtaacggt gaagatccgg
     4081 tggcgatccg taaagcactg caagaatgta tgcagcataa cttctcggtc ttccgtgaag
     4141 gtgatgcgat ggcgaaaggg cttgagcagt tgaaagttat ccgcgagcgt ctgaaaaatg
     4201 cccgtctgga tgacacttcc agcgagttca acacccagcg cgttgagtgc ctggaactgg
     4261 ataacctgat ggaaacggcg tatgcaacgg ctgtttctgc caacttccgt accgaaagcc
     4321 gtggcgcgca tagccgcttc gacttcccgg atcgtgatga tgaaaactgg ctgtgccact
     4381 ccctgtatct gccagagtcg gaatccatga cgcgccgaag cgtcaacatg gaaccgaaac
     4441 tgcgcccggc attcccgccg aagattcgta cttactaatg cggagacagg aaaatgagac
     4501 tcgagttttc aatttatcgc tataacccgg atgttgatga tgctccgcgt atgcaggatt
     4561 acaccctgga agcggaagaa ggtcgcgaca tgatgctgct ggatgcgctt attcagctga
     4621 aagagaaaga tcccagcctg tcgttccgcc gctcctgccg tgaaggtgtg tgcggttccg
     4681 acggtctgaa catgaacggt aagaatggtc tggcctgtat taccccgatt tcggcactca
     4741 accagccggg caagaagatt gtgattcgtc cgctgccagg tttaccggtg atccgcgatt
     4801 tggtggtaga catgggacaa ttctatgcgc aatatgagaa aattaagcct tacctgttga
     4861 ataatggaca aaatccgcca gctcgcgagc atttacagat gccagagcag cgcgaaaaac
     4921 tcgacgggtt gtatgaatgt attctctgcg catgttgttc aacctcttgt ccgtctttct
     4981 ggtggaatcc cgataagttt atcggtccgg caggcttgtt agcggcatat cgtttcctga
     5041 tcgatagccg tgataccgag actgacagcc gcctcgacgg tttgagcgat gcattcagtg
     5101 tattccgctg tcacagcatc atgaactgcg tcagtgtatg tccgaagggg ctgaacccga
     5161 cgcgcgccat cggccatatc aagtcgatgt tgttgcaacg taatgcgtaa accgtaggcc
     5221 tgataagacg cacaagcg
//
LOCUS       DAOFSV010000224         5228 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.224, whole genome shotgun sequence.
ACCESSION   DAOFSV010000224 DAOFSV010000000
VERSION     DAOFSV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.224"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(161..946)
                     /gene="znuB"
                     /locus_tag="QBH27_004504"
     CDS             complement(161..946)
                     /gene="znuB"
                     /locus_tag="QBH27_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDQ7016726.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(943..1698)
                     /gene="znuC"
                     /locus_tag="QBH27_004505"
     CDS             complement(943..1698)
                     /gene="znuC"
                     /locus_tag="QBH27_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDQ7016727.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            1777..2709
                     /gene="znuA"
                     /locus_tag="QBH27_004506"
     CDS             1777..2709
                     /gene="znuA"
                     /locus_tag="QBH27_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDQ7016728.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            2725..4047
                     /gene="mepM"
                     /locus_tag="QBH27_004507"
     CDS             2725..4047
                     /gene="mepM"
                     /locus_tag="QBH27_004507"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDQ7016729.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            4166..5137
                     /gene="lpxM"
                     /locus_tag="QBH27_004508"
                     /gene_synonym="msbB"
     CDS             4166..5137
                     /gene="lpxM"
                     /locus_tag="QBH27_004508"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDQ7016730.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1422 a         1297 c         1325 g         1184 t
ORIGIN      
        1 gttcgtctta tcaggcctac cagagatagc gaaccgtagg tcggataagg catgcggact
       61 gcaggcagag aaaactgacc gattttctct gcactggagg gataatacaa ggtcggataa
      121 ggcgctcgcg ccgcatccga caaatatgtt cagcgaaaga ttagctggcc tgttttttca
      181 tcatactgat aataaataac agtgccgcgc atagcaccac cgacgggcct gcaggtgtat
      241 cgtaaaatgc ggaaaaggtt aaaccgccag tcactgccac catccccacc aaaacagcga
      301 caccagccat ctgttccggc gtgcgggcaa agcgacgtgc agtagcagca ggaataatca
      361 gcagtgaagt aataatcaac gcgccgacga atttcatcgc tacaccaatc gtcaatgccg
      421 tcaccagcat caacaacaat ttcacgcgct gtaatttcac accatcaaca aacgccagat
      481 ccgggctaat cgtcatcgac agcaaattgc gccattgcca gaacaaaata gccaccacga
      541 tgaccacgcc aatcgcaata gagatgagat cttctggcgt cactgccagt aaatcaccga
      601 acaggtaagc catcaaatca acacgaatat tagacatcag actaacgacc accaggccca
      661 gcgacagggc actgtgcgcc ataatcccta ataacgtgtc gatcgccagc tgtggacgct
      721 tctcgagcca taccagaccg cccgccagca gcagcgtaac tgcaatcacc gcatagaatg
      781 gattcacgtc cagcaacaaa ccaaacgcga cgccaagtaa tgaggcatga gccagcgtat
      841 caccgaaata agacatacga cgccagacta caaacgaacc cagcggaccc gcggcacagg
      901 cgagcatgat cccggctaac caaccgggaa ataataattc aatcatgagc gatcatttcc
      961 ccgacgcaaa acaattcgtc cctgtaaatc gtgacgatga ttatgatgat ggcgatagat
     1021 acccagttgt tcagcaccac gaggaccaaa catagaaata aactccggat gcagggaaac
     1081 aacttccggt gtgccggaac aacaaatgtg gtgattcagg caaagcactt catcggtttt
     1141 tgccattacc agatgcagat cgtgagatac cattaaaacg ccacaatcca gttcacgacg
     1201 cagttggtca ataaggtcat ataacgccac ctgaccattc acatccacgc cctgagtggg
     1261 ttcatccagc actaataatt gcggtcgatt taacaatgct cgcgctaaca gtacacgctg
     1321 cgtttcgcca cccgagagct tttgcatcgg tgcgttaatc agatgcccgg cctggacacg
     1381 tttcagtgca ggcaaaatat cttctttatg tgtgccaggg cgtaagcgta aaaaacggtt
     1441 tacggtcagt ggcaacgtgg tgtcgagata cagcttctgc ggtacatagc cgatgcgcag
     1501 ttttccgttg cgcttgataa ccccttcatc gggtgttacc agtccgagca ctacccgtac
     1561 cagtgtcgac ttacctgcgc cgtttggccc aagtaaagtc aaaatttttc caggtttaag
     1621 ttccagcgac acatcagaga ggacgcggcg ttggccaaaa gaaaccgaga cattttccag
     1681 ggaaaccaga cttgtcatgt taattttagt cttgcagtag tcatgaaatg ttataatatc
     1741 acacttctca tattcattac gatgattggt cgcattatgt tacataaaaa aacgcttctt
     1801 ttcgcagcat tatccgccgc tctctggggc ggtgcaacac aggccgcaga tgccgccgtt
     1861 gtcgcttcgc ttaaacccgt tgggttcatc gcttctgcca ttgctgatgg ggtaacagaa
     1921 acgcaggttt tactgcctga cggcgcttca gaacatgatt attcactgcg tccatcggat
     1981 gtaaaacgct tacagaacgc ggacttagtc gtttgggttg gcccggagat ggaagcgttc
     2041 atgcaaaaac cggtaagtaa attaccagaa gcgaaacagg taacgattgc gcagctagaa
     2101 gatgtgaaac cgctgctgat gaaaagtatt cacgacgatg atgatgatca cgatcacgcg
     2161 gaaaaaagtg acgaagatca ccatcacggc gatttcaaca tgcatctttg gctttcccca
     2221 gagatagcgc gggctacagc ggttgcaatc catggaaaat tagtggaact tatgccgcaa
     2281 agtcgagcca aacttgacgc caacctgaag gattttgagg cacaattagc ctcaaccgaa
     2341 acgcaggttg gtaacgagct cgcgccgctc aaggggaaag gttatttcgt ttttcacgat
     2401 gcttacggct acttcgaaaa acagttcgga ctgacaccgc ttggtcattt taccgttaac
     2461 cctgagattc aacctggcgc gcagcgttta catgaaataa gaacacagtt ggttgagcaa
     2521 aaagcaacct gcgtttttgc tgagccacag ttcaggccag cggtcgttga gagcgtcgca
     2581 cgagggacat ccgttcgtat gggaacgttg gatcccctgg ggacgaatat caaactgggt
     2641 aaaacaagct attcagaatt cctgaatcaa ttagccaacc agtatgcgag ctgcctgaaa
     2701 ggagattaat gaggaagtga ttacgtgcaa cagatagccc gctctgtcgc cctggcgttt
     2761 aataatttac cgcgaccaca ccgcgttatg ttggggtcgc tcaccgttct tactctggcc
     2821 gtcgctgtct ggcggcccta tgtttatcac cgtgatgcca cgccaattgt caaaaccatt
     2881 gagctggaac agaacgaaat tcgttcgctc ttacctgaag ccagtgagcc gattgatcaa
     2941 gctgcacaag aagatgaagc cattccccag gacgaactgg atgacaaaat cgccggtgaa
     3001 gcgggcgtgc atgaatatgt tgtttccact ggcgatacgc taagcagcat tctcaatcag
     3061 tatggtattg atatgggtga tatcacccaa ctggctgctg ccgacaaaga attgcgtaac
     3121 ctgaaaatcg gtcaacaact ctcctggaca ttaaccgcgg acggcgaact gcagcgcctc
     3181 acctgggaag tgtctcgtcg tgaaacccga acctatgacc gtactgccgc taacggtttt
     3241 aaaatgacca gcgaaatgca gcaaggagag tgggtcaaca atctgctgaa aggtaccgtc
     3301 gggggaagct ttgttgccag cgccagaaac gccggtttaa ccagcgccga agtgagcgca
     3361 gtgattaaag ccatgcagtg gcaaatggat ttccgcaaac tgaaaaaagg cgatgaattt
     3421 gcggtgttaa tgtcacgaga aatgcttgat ggtaaacgtg agcaaagcca gctgctgggc
     3481 gtacgtttgc gttcagaagg taaagattat tatgcaatcc gcgctgaaga tggcaaattc
     3541 tatgatcgta acggtactgg tctggcgaaa ggattcttgc gattcccgac ggcgaaacag
     3601 ttccgtatct catctaactt taacccgcgt cgtactaatc cggtgaccgg tcgcgttgcg
     3661 ccacacagag gtgttgattt tgccatgcca caaggtacgc cagtgctttc agtgggtgac
     3721 ggtgaagtgg tggttgccaa acgtagtggc gcagcaggtt attatgtggc tattcgtcat
     3781 ggtcgcagct acaccacgcg ttatatgcac ttgcgcaaga tcctggtgaa accgggacag
     3841 aaggtgaaac gtggcgaccg tatcgcgctt tccggtaata ccggacgttc aaccgggccg
     3901 catctgcact atgaagtatg gataaaccag caggccgtaa acccgctgac ggcaaaactg
     3961 ccgcgtaccg aagggctgac cggctccgat cgtcgcgaat tcctggcaca ggccaaagag
     4021 attgtgccgc agctacggtt tgattaatta acatccattc gcagccggta cgcagtcagt
     4081 accggctttt ttatttggtg cggggcaagt tgcgccgcta cactatcacc agattgattt
     4141 ttgccttatc cgaaactgga aaagcatgga aacgaaaaaa aataatagcg aatacattcc
     4201 tgagtttgat aaatcctttc gccacccgcg ctactgggga gcatggctgg gcgtagcagc
     4261 gatggcgggt atcgctttaa cgccgccaaa gttccgtgat cccattctgg cacggctggg
     4321 acgttttgcc ggacgactgg gaaaaagctc acgccgtcgt gcgttaatca atctgtcgct
     4381 ctgctttcca gaacgtagtg aagctgaacg cgaagcgatt gtagatgaga tgtttgccac
     4441 cgcgccgcaa gcgatggtaa tgatggctga gttggcaata cgcgggccgg agaaaattca
     4501 gccgcgcgtt gactggcaag ggctggagat catcgaagag atgcggcgta ataacgagaa
     4561 agtgattttt ctggtgccgc acggttgggc cgtcgatatt cctgccatgc tgatggcctc
     4621 gcaagggcag aaaatggcag cgatgttcca taatcagggc aacccggttt ttgattatgt
     4681 ctggaacacg gtgcgccgtc gctttggtgg tcgtctgcat gcgagaaatg atggtattaa
     4741 accattcatc cagtcggtac gtcagggtta ctggggatat tatttacccg atcaggatca
     4801 tggcccagaa cacagcgaat ttgttgattt ctttgccacc tataaagcga cgttgcccgc
     4861 gattggtcgt ttgatgaaag tgtgccgtgc gcgcgttgta ccgctgtttc cgatttatga
     4921 tggcaagacg catcgtctga cgattcaggt gcgcccaccg atggatgatc tgttagaggc
     4981 ggatgatcat acgattgcgc ggcggatgaa tgaagaagtc gagatttttg ttggtccgcg
     5041 accagaacaa tacacctgga tattaaaatt gctgaaaact cgcaaaccgg gcgaaatcca
     5101 accgtataag cgcaaagatc tttatcccat caaataaaaa atgcctctcg cgaggagagg
     5161 ccttcgactg atgataagtt caagtttgct tcagaagatt cgaaatctgt tgaattatca
     5221 ttgaactg
//
LOCUS       DAOFSV010000225         5205 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.225, whole genome shotgun sequence.
ACCESSION   DAOFSV010000225 DAOFSV010000000
VERSION     DAOFSV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.225"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(28..600)
                     /gene="ppiA"
                     /locus_tag="QBH27_004509"
                     /gene_synonym="rotA"
     CDS             complement(28..600)
                     /gene="ppiA"
                     /locus_tag="QBH27_004509"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDQ7016731.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            871..2052
                     /gene="tsgA"
                     /locus_tag="QBH27_004510"
     CDS             871..2052
                     /gene="tsgA"
                     /locus_tag="QBH27_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDQ7016732.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            2314..4857
                     /gene="nirB"
                     /locus_tag="QBH27_004511"
     CDS             2314..4857
                     /gene="nirB"
                     /locus_tag="QBH27_004511"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDQ7016733.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4854..5180
                     /gene="nirD"
                     /locus_tag="QBH27_004512"
     CDS             4854..5180
                     /gene="nirD"
                     /locus_tag="QBH27_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDQ7016734.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
BASE COUNT         1241 a         1336 c         1366 g         1262 t
ORIGIN      
        1 gggcaagatt gcccgcgcga gaaatcatta cggcaggact ttagcggaaa ggataactac
       61 cggttttgac ggcacattct ggtacggacc aacgtcatga gtcggcacct gggaaatctt
      121 atcggcaacg tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg
      181 accgtggtca aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc
      241 tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcagc
      301 ttcatttttg attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc
      361 ctgaatcata aagccaggaa tgacgcggtg aaaggtcgtg ttgttataaa aaccactgtt
      421 cacataatcg acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc
      481 gatgttacca gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc
      541 gggagaaaga gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat
      601 gagatttcct taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc
      661 aagaacaagg ccatcccttt acctaatttt acgtatctga aatatatgta acctgaggat
      721 aactttttat tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat
      781 aaaacattta atcagatcac aaaatcgcct aaaatcgccc gctcgcggca tagcccgcta
      841 tgccatttta tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca
      901 tggattagct ttctttccta cgcactgacc ggtgcgttgg ttattgtcac cgggatggtg
      961 atgggaaata tcgccgatta tttcaatctg cctgtttcca gcatgagtaa caccttcacc
     1021 ttcctcaatg ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc
     1081 ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg
     1141 atgttcagcc acagcctggc gctgttctcg gcggcgatgt tcattctcgg ggtggtcagc
     1201 ggcatcacca tgtcgattgg tacattcctg gtaacacaaa tgtatgaagg acgtcagcgc
     1261 ggttcacgcc tgttatttac cgactccttc ttcagtatgg ccgggatgat tttcccaatg
     1321 atcgccgcat ttctgctggc gcgtagcatt gagtggtact gggtttatgc ctgcatcggg
     1381 ctggtgtatg tcgctatttt tattctgacc ttcggctgtg agttcccggc gctgggcaaa
     1441 catgcgccca aaacggatgc tccggtagag aaagaaaagt gggggatcgg cgtactgttt
     1501 ctctctgttg cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgccg
     1561 gaatatgcca aaggcctggg catgagcctg aacgacgcag gcacgctggt aagtaacttc
     1621 tggatgtcgt acatggtcgg catgtgggca ttcagcttta ttcttcgctt ctttgatttg
     1681 caacgtatcc tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc
     1741 ggaacaccgg cacatatggc gtggtcaatt ctcgccctgg gcttcttctc cagcgcgatc
     1801 tataccacca tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt
     1861 aactttgtcc tgacctgcgg gaccatcggt actatgttga cctttgtggt taccggcccg
     1921 attgttgaac atagtggtcc gcaggcggcg ctgctgacgg caaacggtct gtacgctgtc
     1981 gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg
     2041 acctctcatt aattgttcat gccgggcggc actatcgtcg tccggccttt tcctctctta
     2101 ccctgctacg tgcatctatt tctataaacc cgttcaattt gtctgttttt tgcacaaaca
     2161 tgaaatatca gacaattccg tgacttaaga aaatttatac aaatcagcaa tatacccctt
     2221 aaggagtata taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag
     2281 gtaggcggta atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc
     2341 ggtaacggta tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc
     2401 aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc
     2461 tcgtcttact tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac
     2521 gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta tcaccatcaa ccgtcaggag
     2581 aaggtgattc actccagcgc cggacgtacc gttttttatg acaagctgat catggcgacc
     2641 ggttcctacc cgtggatccc gccaatcaaa ggttctgata ctcaggactg ctttgtctat
     2701 cgcactattg aagacctcaa cgccattgaa tcctgcgccc gtcgcagcaa acgcggtgcc
     2761 gttgttggtg gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggcatt
     2821 gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggc
     2881 ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac
     2941 actcttgaga ttgtgcagga aggtgttgaa gcgcgtaaaa ccatgcgttt tgccgacggc
     3001 agcgaactgg aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg
     3061 gcaacccagt gtggtctgga cgttgctccg cgtgggggta tcgtcataaa tgattcctgc
     3121 cagacttccg atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta
     3181 tttggtctgg tagcaccagg ctacaaaatg gcgcaggtcg ccgttgacca tattcttggt
     3241 agcgaaaacg cctttgaagg tgcagacctt agcgccaagc tgaaactgct gggcgtagac
     3301 gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg cacgtagcta cgtttacctc
     3361 gacgaaagca aagagatcta caaacgcctg attgtcagcg aagacaacaa aaccctgctc
     3421 ggtgcggtac tggtgggcga taccagcgac tacggcaacc tgctgcaact ggtgctgaac
     3481 gctatcgaac tgccggaaaa cccggattct ctgattctgc cggcgcactc gggtagcggt
     3541 aagccgtcta tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac
     3601 gtcaccaaag gcgacctgat tgctgctatc aacaaaggct gccacaccgt tgcggcgctg
     3661 aaagccgaaa ccaaagccgg tacgggctgc ggcggttgta ttccgctggt cactcaggta
     3721 ctgaacgcgg aactggcgaa acagggtatc gaagttaaca acaacctgtg tgaacacttt
     3781 gcttattcgc gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa
     3841 gaactgctgg cgaaacacgg caaaggttac ggttgtgaag tttgtaaacc gaccgtcggt
     3901 tcgctgctgg cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag
     3961 gattctaacg acaacttcct cgctaacatc caaaaagacg gcacctactc ggtgatcccg
     4021 cgttctccgg gtggtgaaat caccccggaa gggctgatgg cagtgggtcg tatcgcgcgt
     4081 gaatttaatc tctacaccaa aatcaccggc tcccagcgcc tggcgatgtt tggcgcacag
     4141 aaagacgatc tgccggagat ctggcgtcag ctgattgaag caggtttcga aaccggtcat
     4201 gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg gtagcacctg gtgccgctat
     4261 ggcgttggcg acagcgtcgg cctcggcgtg gaactggaaa atcgctacaa aggcatccgt
     4321 acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagctcag
     4381 ggtaaagacg tgggtattat cgccactgaa aaaggctgga acctgtatgt ttgcggtaac
     4441 ggcggcatga aaccgcgtca tgcggatctg ctggcagcgg atatcgatcg cgaaacgctg
     4501 attaaatatc ttgaccgctt catgatgttc tacatccgta ctgccgacaa actgacgcgt
     4561 accgcgccgt ggttagaaaa cctcgaaggt ggcatcgatt acctgaaagc ggtgatcatt
     4621 gacgacaaac tggggctgaa cgcacatctg gaagaagaga tggcgcgcct gcgtgaagcg
     4681 gtagtgtgtg agtggactga aacggtcaat accccgtctg cgcagactcg cttcaaacac
     4741 ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac
     4801 cgtccggcaa cgccgtatga acgtatcccg gtaactctgg tggaggacaa cgcatgagcc
     4861 agtggaaaga catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt
     4921 taggtgacga acaagtagca attttccgcc cgtatcacag cgaccaggtg tttgcgatca
     4981 gcaacatcga tccgttcttc gagtccagcg tgctgtcacg cgggctgatt gcggaacacc
     5041 agggcgagct gtgggtcgcc agcccgctga aaaagcagcg ttttcgctta agcgacggct
     5101 tgtgcatgga agacgaacag ttttccgtca agcattacga cgcgcgagtg aaagacggcg
     5161 tggtgcagct gcgcggttaa tattttaacg ggaggcgcaa tgcct
//
LOCUS       DAOFSV010000226         5175 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.226, whole genome shotgun sequence.
ACCESSION   DAOFSV010000226 DAOFSV010000000
VERSION     DAOFSV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.226"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            611..1327
                     /gene="nanC"
                     /locus_tag="QBH27_004513"
     CDS             611..1327
                     /gene="nanC"
                     /locus_tag="QBH27_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDQ7016735.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1347..2453
                     /gene="nanM"
                     /locus_tag="QBH27_004514"
     CDS             1347..2453
                     /gene="nanM"
                     /locus_tag="QBH27_004514"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDQ7016736.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGDLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            2517..3497
                     /locus_tag="QBH27_004515"
     CDS             2517..3497
                     /locus_tag="QBH27_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDQ7016737.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFTHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(4080..>5175)
                     /locus_tag="QBH27_004516"
     CDS             complement(4080..>5175)
                     /locus_tag="QBH27_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDQ7016738.1"
                     /translation="GYCNSLCYHGKLQPKRGMEKGTIFPAMGYLHIDGRGMKPNGGSR
                     YNPLEAETIAAWLVAHKDDIERHYGEPLYKVVGVVTPFSAQVNAIKTSLRKLEINGKD
                     EQGSLTVGTVHSLQGAERAIVLFSPVYSKHEDGRFLDSNSSILNVAVSRAKDSFLVFG
                     DMDLIEMQPAFSPRGLLAKYLFSSDNNALQFEFQKRQDLISAHTQISTLHGVEQHDGF
                     LNKTLAGAQKKITIISPWLSWQKVEQTGFLASMALARSRGIDITVVTDKNCNIAHVDD
                     DKRQEKQHLLNDAVEKLNKMGIATKLVNRVHSKIVIEDEELLCVGSFNWFSAAREDKY
                     QRYDTSLVYRGEGVKNEIKAIYGSLDQRQL"
BASE COUNT         1522 a         1133 c         1123 g         1397 t
ORIGIN      
        1 aataaattat ttataaaaca tatagttaaa agaaaatctt tagtttttga agaccaccgc
       61 aagtgttcgt ctggcttcac atggcatctg cctcttagaa aaagatcgac atattttgtg
      121 acacgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc tggtataaca
      181 ggtataaagg tgcaccacga tagtcaatga aactgggcat ctcgcaatct atggcaaaca
      241 tcacttcagt tctttctcat cgggtgatga aaacgcactt cagtctgaaa ggaatatgaa
      301 aatgagatca acagacactc tattttatga ctctgggtaa aatggattga gtaagcgata
      361 tagcttacga aaattcaaat caattaaaca tcagcagaga ttttatactc aggtatttaa
      421 tctggctctc tgtttattta aataatgtgg aaagagattt ttcacaggag acattataca
      481 aaaaatataa aatacagcta cccgttgcca aagacactat aagcctggca aaaaatatta
      541 cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg
      601 agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc
      661 cccattaatt tctcaggctg cgacactgga cgtacgtggt ggatatcgta gtggaagcca
      721 cgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat
      781 ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga
      841 tgttcaggtt gaagttaatt acgcaattaa acttgatgat caatggacgg tacgcccggg
      901 aatgttaacg cattttagca gcaacggcac acgttacgga ccctacgtaa aactgtcctg
      961 ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg
     1021 acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt
     1081 cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca
     1141 gaacgattat cgctatgcaa accataagaa atgggcgacg gaaaatgcat ttgttctaca
     1201 ataccatatg acgcccgata ttacgccata catagaatat gactaccttg accgtcaggg
     1261 tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa
     1321 actgtagtag acaggagaca gtcacaatga ataaaacaat aacggcgctt gctatcatga
     1381 tggcttcatt tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg
     1441 gtaccggagc aattgataac gacactgtct acattggttt aggtagcgca ggtacggcat
     1501 ggtacaagct ggatacacag gccaaagata aaaaatggac agcgttagct gcattccctg
     1561 gtggaccaag agatcaagca acctcggcat ttattgatgg tgatctgtat gtgtttggcg
     1621 gcattggcaa aaatagcaag ggcttgactc aggtatttaa tgacgtacac aaatacaacc
     1681 ccaaaaccaa tagctgggtt aaattgatgt cgcacgcgcc aatgggcatg gcgggccatg
     1741 tgacttttgt acacaacggc aaagcttatg ttactggcgg tgttaaccag aatatcttca
     1801 atggctattt tgaagatctc aacgaggctg gaaaagattc aaccgctata gataaaatca
     1861 acgctcacta ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt
     1921 ttgatccctc aacccagcaa tggagttacg ctggcgaatc tccctggtac ggaacggctg
     1981 gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaacccg
     2041 gattgcgaac ggatgccgta tttgaacttg atttcaccgg tgataattta aaatggaata
     2101 ggcttgctcc cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg
     2161 attctcttat atttgccgga ggggccggat tcaaaggttc acgagaaaat taccagaacg
     2221 gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt catctttggc
     2281 ataacgggaa atgggataaa tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat
     2341 tgccctggaa taatagtcta ttgattattg gcggtgaatc tgcaggcggc aaagcggtga
     2401 cggattcagt tttgatctct gtgaaggata ataaagtcac agtacaaaac taacgcttca
     2461 gggccccggt aaagggccct gctatcaatt tgtcatttca attacgcgaa aattatatga
     2521 acgcaataat atcgcccaat tattactatg ttcttaccgt tgctggtcag tctaatgcca
     2581 tggcgtatgg cgaaggactg ccattaccgg acagggaaga tgcgcctcat cccagaatca
     2641 aacaattagc gagatttacg catacgcatc ccggaggccc gccatgtcac tttaacgaca
     2701 ttattccact gactcactgc ccacacgatg ttcaggatat gcagggttat caccatcctc
     2761 tggcaacgaa tcatcaaaca cagtacggca ccgttggcca ggcactgcat attgcacgga
     2821 aattactgcc cttcattcct gataatgcag ggattctcat cgtcccgtgt tgccgtggcg
     2881 gatcggcttt taccgcgggc agcgagggga catattcaga acggcacgga gccagccatg
     2941 atgcttgtcg ttggggaacg gataccccgc tctaccagga tttagtcagc agaacgcgag
     3001 ccgcactggc aaaaaatccg cataacaaat tcctcggcgt atgctggatg caaggcgaat
     3061 ttgacttaat gaccagtgac tacgcgtcac accctcaaca ctttaatcat atggttgaag
     3121 cctttcgtag ggatctaaaa caattccatt ctcagcttaa taatattact gccgcgccgt
     3181 ggttttgcgg cgataccacc tggtactgga aagaaaattt ccctcatgcg tatgaagcta
     3241 tttatggcaa ttatcaaaat aatgttttag ccaatattat tttcgtcgac ttccagcaac
     3301 aaggtgaaag aggactgacg aacgcgcctg atgaagatcc ggacgattta agcacgggat
     3361 atttcggttc agcgtaccgg tcagcggaga actggacgac gtcactgcga agcagtcatt
     3421 tcagcgcggc agcccgtcgg gggattattt ctgacagatt tgtagaagca attttgcagt
     3481 tttggcgcga aaggtgagcg gggagtttat tcatggggga tgtccttgtc cattcccaga
     3541 gcagacaaca aaaaacaaat gagatcaagg taatatctct tcacttgcgg gctcgaacag
     3601 caggcaattg ccaatataat aatctggcac aagggtgaaa aacgcgcacc gcataaaccg
     3661 ttgttattgc ttacagatta gccggatgcc ttaatagaca tccgcgcctt ttcggttatg
     3721 gctcggaaat ctacgaacat ctacacagtt tactggaacg ttttgggcca cagcgttcgc
     3781 agcaccgtcc tgatatgccg ttctggcgat tataagtcga cggattctgg caaatagacg
     3841 atgatgatat gcttaatgat gttcaaagaa attaggccat tattatatta ttgcttgcat
     3901 tttctttcat tcctaaaagc acccgccatt aatcccaggc gcattatgca aaggaaaatg
     3961 attaggtaaa caataagatt tataatcagg aaaggcgact cgcaataaaa cggaaagtcg
     4021 ccttaacatt ggtaaaatat gccctttact actcctgtat catgaacggc tcatcacatt
     4081 taaagctgac gttgatccag actaccatat attgccttta tttcattttt tacgccttcg
     4141 ccacggtaca ccaaagacgt atcgtatcgt tgatacttat cttcccgtgc agcactgaac
     4201 cagttaaaag atccgacaca cagcagttct tcatcctcaa tcacaatttt gctgtggaca
     4261 cgattaacca atttcgtagc gatacccatc ttattgagct tttcaacagc atcattcagc
     4321 agatgttgtt tctcctgacg cttatcatca tcgacatgcg ctatattgca gtttttatcc
     4381 gtcaccacgg taatatcaat gccccgggaa cgggccaaag ccattgatgc cagaaatccc
     4441 gtttgctcca ctttttgcca agataaccaa ggggaaataa tcgttatttt cttttgagct
     4501 ccggcaagag tcttgttcaa aaatccatca tgctgctcca caccatgcag cgttgaaatt
     4561 tgtgtatgtg cggaaattaa atcctgtcgt ttctgaaact caaattgcaa cgcattatta
     4621 tctgaagaaa agagatattt tgccaataac cctcgcggcg aaaatgcggg ctgcatctcg
     4681 ataaggtcca tatcgccaaa taccagaaaa ctatctttag cgcgtgagac agcaacattg
     4741 aggatactgc tgttgctatc aagaaacctg ccatcttcat gttttgagta gacaggagaa
     4801 aagagaacaa tcgccctttc cgccccctgc aaggagtgca cagtccccac tgtcagtgaa
     4861 ccctgctcgt ctttgccatt aatttccagc ttacgcaatg acgttttgat agcattaacc
     4921 tgcgccgaaa atggcgtcac aacaccaaca actttgtaca gtggttcccc gtaatggcgc
     4981 tctatatcat ccttatgtgc caccagccat gctgctattg tttcagcttc aagagggtta
     5041 taacggcttc ctccatttgg cttcatgcct ctaccatcaa tatgtaaata tcccattgcg
     5101 gggaaaatcg tccctttttc catccctctt ttaggctgca acttaccgtg atagcaaagt
     5161 gagttacagt agccg
//
LOCUS       DAOFSV010000227         5167 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.227, whole genome shotgun sequence.
ACCESSION   DAOFSV010000227 DAOFSV010000000
VERSION     DAOFSV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.227"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            1310..1519
                     /locus_tag="QBH27_004517"
     CDS             1310..1519
                     /locus_tag="QBH27_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016739.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="QBH27_004518"
     CDS             complement(1585..2118)
                     /locus_tag="QBH27_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016740.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="QBH27_004519"
     CDS             complement(2121..3047)
                     /locus_tag="QBH27_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDQ7016741.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="QBH27_004520"
     CDS             3224..3622
                     /locus_tag="QBH27_004520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016742.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="QBH27_004521"
     CDS             complement(3826..4740)
                     /locus_tag="QBH27_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDQ7016743.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1436 a         1351 c         1104 g         1276 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg taagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DAOFSV010000228         5128 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.228, whole genome shotgun sequence.
ACCESSION   DAOFSV010000228 DAOFSV010000000
VERSION     DAOFSV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.228"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..309)
                     /locus_tag="QBH27_004522"
     CDS             complement(<1..309)
                     /locus_tag="QBH27_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDQ7016744.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     M"
     gene            complement(481..1539)
                     /locus_tag="QBH27_004523"
     CDS             complement(481..1539)
                     /locus_tag="QBH27_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ7016745.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVLETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(1737..2210)
                     /gene="sbmC"
                     /locus_tag="QBH27_004524"
     CDS             complement(1737..2210)
                     /gene="sbmC"
                     /locus_tag="QBH27_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDQ7016746.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQMVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(2329..3495)
                     /gene="dacD"
                     /locus_tag="QBH27_004525"
     CDS             complement(2329..3495)
                     /gene="dacD"
                     /locus_tag="QBH27_004525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDQ7016747.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            3704..>5128
                     /gene="sbcB"
                     /locus_tag="QBH27_004526"
     CDS             3704..>5128
                     /gene="sbcB"
                     /locus_tag="QBH27_004526"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDQ7016748.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
BASE COUNT         1340 a         1306 c         1191 g         1291 t
ORIGIN      
        1 catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg agagctcggc
       61 atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc tggcgatgat
      121 gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt tgtccagcaa
      181 caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt cacgttgcag
      241 tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg aaggcttggt
      301 agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc aaccgctatt
      361 cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc ccttttcact
      421 atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga agttcaggaa
      481 ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat gtgccgtttc
      541 catgttcagc cacacataac catagattgg tgtttccaag actttcaggt catggtggtt
      601 atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat tggcaaaaac
      661 cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt gctgcatcat
      721 gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg ggcgcgtggc
      781 ccagtatgca ttgatttgca actccagcat acaaaccaga ttgcgattaa tggtctggat
      841 accttcatat atcgatttgg gaatacgggt ttctttgctg gcgggcgcaa tcagcccacg
      901 cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg ggcgttcgag
      961 taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac ttttcgccag
     1021 ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca acattgccag
     1081 taaagagccg aggatcacat cgccgcttcg ccataacgcc gtatcaattt cacctgtcgg
     1141 ggaacccaca acaattgcca gcgtcacccc aatcaataat ccttgatacg gtttcttgcc
     1201 cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac cagactaaca tcagcggtaa
     1261 cgaaattaac tccagttgca gagcgataag acctaaaatc gaacccaaca ccgtaccgcc
     1321 aatacgttcg aaggcacgag ggacaacgtt cccccagaac gagattggcc ccataatcac
     1381 caccatggtg accagcggcc aggtgctttc cgggatagta aacaggcgga taatgagaaa
     1441 agtgagcagg aatgccagcg cgacccgagt accatgcaca atgcggtaat ggcggtatac
     1501 ccggatttca aacgggctta atgacttatc ggcacgcacg cagaaactcc gtaatgaaga
     1561 tgaaaagcgc tcataacaca tctgtcggcc agaaggcgcg cctctgagtt atccgacgga
     1621 cataccggga acagtgtaat ttcccggcag gagcgaaaaa tttgaaaggc gcatcttact
     1681 cttttcgctt tcaaaaaaag agtggtcttc gcgttaacac accgccctga gatgaattag
     1741 tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc ttccgcgcca
     1801 ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact gtcctgcaag
     1861 aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac acgagccacc
     1921 gccaccgcat actgaccacc tgaaatttct gtcagaatga cgccctcact gttttcgggg
     1981 agcgtaaagt tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt
     2041 tcatctggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt tttgctatct
     2101 atccacatca tcaactgctc aaaacctttc tttaccatct gctcccacgg gccaacaaga
     2161 tggaaacctg caacggtacg tttatcttcc tgcttaatct cgtagttcat gacgcctcca
     2221 ctaacactgt ttttatatac agtatagttg caatattaaa accacaagga atgagtgttg
     2281 attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc aggccttatg
     2341 gtggaaataa tcactcaggc gggaaaacat gctgccttcc ccgacagatt ccagggtaac
     2401 cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg
     2461 atgggcgcta attggcgcgg tgagttcttt accatcaagg gtatatttgg ctttgatatg
     2521 tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccca gggcgatatt
     2581 ttctttatca ccataccaga tgcgttccgt accgaccttt ttcccacggt gcaaaatttg
     2641 caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt cctcacgacc
     2701 ttttgcgctg tcagccccca ttaccactgc aatgaggcga cgctgcccgt ctacagccga
     2761 agcaataaga ttaaacccgg caccagaagt atggcccgtt ttcaggccgt caacattcat
     2821 ggttttatcc cacaataacc cgttacggtt ttgctgggtg ataccgttcc aggtgaggct
     2881 tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac
     2941 ggctaaatca taagccgagc tatgctggcc aggtgcatcc agaccatgca ctgtttcaaa
     3001 atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa caaactgccg
     3061 ttgcccaccg gcaatatagt cagccagagc aacacaagcg tcatttccgg aatccacaat
     3121 taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa acatcagtga
     3181 agaaccgaca aacaccggat tatctttcgc ccacgcatca cgccccacgg tgacaatatc
     3241 gtctggcgta atgcgatgac tatcgatagc gcgatccacg acatagcccg tcatcagctt
     3301 tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagaa tctgaccggt
     3361 ggtgtaatcc atcaataccc aggacccggc atgaatctct ggaggctgag gtgaaaaagg
     3421 aatgttttcc gccgcaaaac cagacgataa attaaaaacg aacaaagaag cagcaataat
     3481 aagacggcgt ttcaacagca aaccctcagg tgtttcaaat agctgttctt tttacggaaa
     3541 tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctactatctc atttttatct
     3601 gacatgatct gttgccactc gctgccaaat tgtcgcgcta aagctgatta gcacggtgat
     3661 atttgatact ctggcagaca gcagaaataa cggatttaac ctaatgatga atgacggtaa
     3721 gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggaacgcacc ccgcgttaga
     3781 tcgccctgca cagttcgcag ccattcgcac tgataacgaa ttcaatgtca tcggcgaacc
     3841 cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cagcctggag cagtattaat
     3901 taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg cgtttgccgc
     3961 ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg
     4021 tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc cttacgcctg
     4081 gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg cctgttatgc
     4141 cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga gctttcgcct
     4201 tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg cgatggctga
     4261 tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac gcctgtttga
     4321 ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg ttccgcagat
     4381 gaaaccccta gttcacgttt ccggaatgtt tggggcatgg cgtggcaata ccagctgggt
     4441 ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attttggtgg atttggcagg
     4501 agacatttcg ccattactgg agctggatag cgacacattg cgcgagcgtt tatataccgc
     4561 aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata tcaataaatg
     4621 tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac tgggaattaa
     4681 tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag tgcgtgaaaa
     4741 agtggtggcg atattcgcgg aagccgaacc gtttacacct tcagataacg tggatgcaca
     4801 gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg tgctggaaac
     4861 cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa ttgaaaaact
     4921 gttgttcaat tatcgagcgc gcaacttccc ggggacgctg gattatgccg agcagcaacg
     4981 ctggctggag caccgccgcc aggtcttcac gccagagttt ttgcagggct atgctgatga
     5041 attgcagatg ctggcacaac agtatgccga tgacaaagag aaagtggcgc tgttaaaagc
     5101 gctttggcag tacgcggaag agatcgtc
//
LOCUS       DAOFSV010000229         5073 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.229, whole genome shotgun sequence.
ACCESSION   DAOFSV010000229 DAOFSV010000000
VERSION     DAOFSV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.229"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..143
                     /locus_tag="QBH27_004527"
     CDS             <1..143
                     /locus_tag="QBH27_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltranspeptidase"
                     /protein_id="HDQ7016749.1"
                     /translation="KLLEAKGQKVALNEAMGSTQSIMVGSDGELYGASDSRLVDDLTA
                     GY"
     gene            complement(183..1217)
                     /locus_tag="QBH27_004528"
     CDS             complement(183..1217)
                     /locus_tag="QBH27_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDQ7016750.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(1220..2185)
                     /locus_tag="QBH27_004529"
     CDS             complement(1220..2185)
                     /locus_tag="QBH27_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ7016751.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(2239..2997)
                     /locus_tag="QBH27_004530"
     CDS             complement(2239..2997)
                     /locus_tag="QBH27_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDQ7016752.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(3011..4225)
                     /locus_tag="QBH27_004531"
     CDS             complement(3011..4225)
                     /locus_tag="QBH27_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDQ7016753.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(4421..4759)
                     /locus_tag="QBH27_004532"
                     /pseudo
     CDS             complement(4421..4759)
                     /locus_tag="QBH27_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1414 a         1143 c         1109 g         1407 t
ORIGIN      
        1 tcaagctgct ggaagcaaaa ggtcagaaag tggcgctgaa tgaggcgatg ggcagcacgc
       61 aaagcattat ggttgggtcg gatggtgagt tgtacggcgc atccgactcg cgcttggtgg
      121 atgatttaac ggcggggtat taattgacat cccccggcgc agtgtgccgg gggataatgg
      181 ttttatgcgg tcaataatcg tccgggatta tcgatacaga gttttttgag gatcgtttta
      241 tcgacgccgc gctccgccag ataggccaga aaaacatctg gaacaaatcc ccagccatta
      301 ccgccatttt tagcccacat ttgtttaagg aagacatcgt ggctgagaac aagctgatcg
      361 gcgtatccca actcaatgag atgagcgacg gcatcggcag tctcctgcac ccctggcgct
      421 attccctctt tcgggaaggt aatgtctagg ccaatcatgt cgaattccag ccagacaccc
      481 ttatcaagca ttttccgctg atacgccacg tcttttcctg acggatccga gtgtgcgaga
      541 gagactttat ttggcgagac gcccatttca cctaacacaa tgtcgagtac ttcatcaccg
      601 cgacgaagcc agcccggcat gtgaatattc atcgccacat gaggattgtt aatctgtgcc
      661 agcgaggcag cccgcaagct gttatgctcg gcttcggtaa atgtcggtga gacacctatt
      721 tcaccgatca ttccggcacg aatatccgta tcgccaatcc cttgattcaa ttctttatcg
      781 atcgttgccg ccagttcatc aaccgtttta tgaattctct ggctttcaaa tttttccagg
      841 tagggaccgg aggaagcaac aatattcaga cctgttttta atgcgacttc tcgtaatgcc
      901 tgcgcatctc gtccgataga ctcagaccct gtggcatcaa caatggtgcg accaccgagc
      961 gagataaagt tattaatttc gaagatcaca tcttcaatag gtttgcggtc catattatca
     1021 gcgcagcaat aaggatcgtg ttttaatgcc cactggattt ctgccgtgac ttttttatca
     1081 acaagccgtt gtgaaaaagg atagcaagga gcatccacaa cagaggaaag atcgttgaac
     1141 agatgttcat gaggaagtgt tagccccata tcttcacggg cgacggggcc cgtcacagtt
     1201 tgtagataat ctttcatgat tatacctcca ttttcttcat tgttttcgag gttctgtatt
     1261 ccggaatagt ggtaacaatt gcaccgacca gtgcaaacag tgttccgatg atcgtgacca
     1321 gataaacggt attgcccagc gatggaatta atacgtcaat caatactgat cccagtaact
     1381 ggcccgccgt tgaagcaacc cccagcatca gtaaccccag acctctgacc aaaatggcca
     1441 ttaaaccgat agacattagt ccaagcgggc cgccaagata catccaccag acatctggta
     1501 attgaacagt gacgtgccct aatgcgacac gaatagctag cgctgtgcct aaaacacaaa
     1561 atccaacgat gaaattccag gtgattgaaa ccagcatgga tcccgtagct tcagctactt
     1621 ttgcatttcc agcgggctgc catcctgcaa gcaaaccagc taagaacgga agaatggcaa
     1681 gtaagatgaa agaggtagaa tgccactgcg gtgacacaac aaaaatcgta gcaataaccg
     1741 caaataatgc accgatgatg cgataagggg taaagtattt tttctcgtcg acaccgatac
     1801 caaagcgatc gcaaagtaaa ccagacagca atagcgcgga gatcagtgcg gtttgaaacg
     1861 ttgcgacccc gagtgcgctg gctgatgcgc cttcagaaaa taccaccatc gctccacaga
     1921 gaccggcaaa ccagttccag agaggaactt ttcggttttt aatcagagaa ggaattgccg
     1981 caaattgttg ccgggtttct tttcgcgcca taataataaa aaacatcacg actaaaccac
     2041 tggcaaagga gataactgcg catgcattac catcctgtaa ccagtgacct aattgaccat
     2101 tcaccgctga ttgcataggg gagagcatac ccgcaagaat cgtggcaagc atccataagg
     2161 gagttgagta cttattcttt ttcatacatc aatccttcta ttatagtaaa tggggtaact
     2221 ggagttaccc cattattatt atcttgcaat atgagtcagt atatttaccc acaacgcggt
     2281 atagaacggc caggacataa actgctgtgt accccagtgc ggtgaacagt cactcataaa
     2341 gcaggctatt ttcccgttgt gatagttacc aaagaccagt aatggatcgt tgtttattgt
     2401 taataccacc tcagcatttt ctttagcgat cgcccggtta taacctagga aaaaaggata
     2461 ttcagagaac cctttaataa ctggatgttc aggttgtgat ggctgtgcaa taacaccctc
     2521 tggtttttcg acacgatcgt caccatctaa catcgtaacc gggagcacat cagccagaac
     2581 tgtattttta taattggctt ttgcttcaat tcccatgaat gagagatagc cgccaatcat
     2641 caataaccca ccaccgttat tcacatattc tttaatcagt tcgagagcgt ttggttttat
     2701 gcgaagttga taaaaagtat cattttgtag caggaaagta ttactaccaa tatcactaat
     2761 aacgatggca tcatattgtt ctaactgtgc cacatcttca gggaaggcaa tttgtaccgt
     2821 atgtgccggc atataggtga catctacctg actatttttc agacattgga gtaaccaggt
     2881 cgcaccttct tcatatttac ttgaggtaaa actgtcatat ccttttgaat gaatcatatg
     2941 aatatgccag gattccccaa taaagagcac ttttagtgat tttttggtgt tattcattct
     3001 ctgtcccttc ttaatgcgta aaaatagttt gctgataatg tatactctga attcgatgta
     3061 tcacagattc atgttcaggc atgtcggaag cattgcttgt ctccaccgct agggatgcaa
     3121 aggcgctggc ataacacagt gctgattcca gtgattttcc tttcgcaagt cctgatgcca
     3181 gagcgccgtt aaaagcatcc ccggctcctg ccgtattttt aacgaccgct ggaaacgctg
     3241 gcgaatgaat gaattttttg ccatcaaatg cgagcgagcc cttgctaccg agcgtgataa
     3301 ccgtgttttt taccccttta ttgtggatag cttctgcagc tcgtttagct gattcaagat
     3361 cgtgtacgtc tatgccggac agtaaaccag cttctgtttc attgggggtg atataatcaa
     3421 catcctgaat aagttcgttg acgatatcat tatagggtgc cgggttaata atgaccggga
     3481 tactgttttt ttgcgccaat gtgatcgctt gttgcagagc ggtataattt gtttcgagct
     3541 gcagaagtat gacatcagaa ttaataatcg cttctttctg aatagttatt tcatcggacg
     3601 acattgtcat gttggcaccg ggatagatcg caataacatt atcgccagta ccttcattaa
     3661 caaggatcgt ggcagttccg gtttgcgttt ctttcgtttg ataaattata cttttataaa
     3721 tcttagacga attcataaag ttgatcgcat aatcactaaa gtgatctgta cctacttttg
     3781 ttatgaaata aacatcagta tcggcaaagc ttgctgccag cgcttggtta cagccttttc
     3841 cccccggtga aaatataaaa ttacttgcca gaagcgattc tccgatagtg ggaagcctcg
     3901 gcaaataact gatgatatca acattaaacg aaccaataac gcatacttga cttttcatag
     3961 ttgagtggcc tttttttatt ccctgggctg tatcataatt ttcaagcgca taagcgattt
     4021 catttttcgc aatagtatca agcgagtcta ggacgataaa agcaccgcca tgacatcgag
     4081 tcagatagcc aagcctggca agttcattaa gatctgatcg aatagtttct ttcgttacat
     4141 tgaggctatt ggacagatcg gatactttta cagaaccgaa tttactgagt cgtttcaata
     4201 tttcatgatg gcgttcaaat ttcatgcttc acctcacttc agttacgtta atatctatcg
     4261 atgtatattg atgtgataat caccataaag tggcggggaa aagcaaaaaa aagcaaaaat
     4321 agcctgagaa aaggtaagcg ttaatggaga atcatattga tgcttattta ttggtgagaa
     4381 ctacgttcca tggcaggagt tcatcaatac ggttggaagg ttattccggc agtacgctca
     4441 ggatatggcg cagatacgct tccggatcaa tgtcgttcag aaggcaagtg ccgataagcc
     4501 catacaacag tgcactgcgc tcgccgccga agaacataaa gtttttattt ccgagacaga
     4561 tggcacaaag ggctttttcc gcggcattat gtctgcctcc gcctgaccgt caacactgta
     4621 ataacagagt gcgccccact gattcagtac acagctgaac cattcaccca gtctggattt
     4681 ttggatagcg tggcattttt ctccaccatg catgcagtga gtccagtaac agtttgctct
     4741 tcttctgcct gaccacaaga tgtgctggac tggtaaacga tggggatacc tgcttacgcg
     4801 agagcaataa gtggctggcc acaattctcg gtacaaaact ctgctgaata gcagcgattt
     4861 tcccagcgtg gtgcgtcgtt tgcattttac tggttctaag ttaacaatta tctgttcact
     4921 aatgactact ctaaaaactt gtatcaggac tatcacttat ttaagtgata ttggttgtct
     4981 ggagattcag ggggcctgtc tagagagata taaagatacc ttcaccccgg tgatgataaa
     5041 ataaaagtat ctcagctatt aaactgatgt acg
//
LOCUS       DAOFSV010000230         5033 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.230, whole genome shotgun sequence.
ACCESSION   DAOFSV010000230 DAOFSV010000000
VERSION     DAOFSV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.230"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..202
                     /locus_tag="QBH27_004533"
     CDS             <1..202
                     /locus_tag="QBH27_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDQ7016754.1"
                     /translation="KLITNNINVLGSEIYSESRFFIIGHDDWDKLEYFINSSVKPMDY
                     DSLYKFSPDKMMSTIVYDFIEK"
     gene            246..1325
                     /gene="waaB"
                     /locus_tag="QBH27_004534"
                     /gene_synonym="rfaB"
     CDS             246..1325
                     /gene="waaB"
                     /locus_tag="QBH27_004534"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HDQ7016755.1"
                     /translation="MKIAFIGEAVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDK
                     MDKGWLEGIKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKA
                     RKKSGIDVPVFSWPHFSLDHKKHAEYITCADYHLAISSGIKQQMINRGVPESTINVIF
                     NPVEAKDTVIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGS
                     DFEKCQAYGRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESM
                     SWGIPCISADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHIEHEKIPAS
                     IDKFYQSKYYDRLHNIIISAISRRK"
     gene            1325..2344
                     /gene="waaO"
                     /locus_tag="QBH27_004535"
                     /gene_synonym="rfaI"
     CDS             1325..2344
                     /gene="waaO"
                     /locus_tag="QBH27_004535"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDQ7016756.1"
                     /translation="MQQVFFQETEYLNSVIDYNHKVETENLCLDIAYGTDKNFLFGCG
                     ISIASILKYNEGSRLCFHIFTDYFGDNDRKYFDALALQYKTRIKIYLINGDRLRSLPS
                     TKNWTHAIYFRFVIADYFISKVAKVLYLDADIICQGTIEPLIKFSFPDDKVAMVVTEG
                     QADWWEKRAHSLGVAGISKGYFNSGFLLINTAKWAAQQVSARAIAMLNEPEVIKKITH
                     PDQDVLNMLLADKLIFADIKYNTQFSLNYQLKESFINPVTNNTIFIHYIGPTKPWHDW
                     AWDYPVSQAFMEAKNASPWKNTALLKPNNSNQLRYSAKHMLKKHRYLKGFSNYLFYFI
                     EKIKH"
     gene            2384..3400
                     /gene="rfaJ"
                     /locus_tag="QBH27_004536"
     CDS             2384..3400
                     /gene="rfaJ"
                     /locus_tag="QBH27_004536"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase RfaJ"
                     /protein_id="HDQ7016757.1"
                     /translation="MDSFPAIEIDKVKAWDFRLANINTSECLNVAYGVDANYLDGVGV
                     SITSIVLNNRHINLDFYIIADVYNDAFFQKVAKLAEQYQLRITLYRINTDKLQCLPCT
                     QVWSRAMYFRLFAFQLLGLTLDRLLYLDADVVCKGDISQLLHLDLNGAVAAVVKDVDP
                     MQEKAASRLSDPELLGQYFNSGVVYLDLKKWANAKLTEKALSILMSKDNVYKYPDQDV
                     MNVLLKGMTIFLPRGYNTIYTIKSELKDKTHQNYKKLIIENTLLIHYTGATKPWHKWA
                     IYPSTKYYKIALDNSPWKDDSPRDAKSIIEFKKRYKHLLVQHHYISGIIAGVCYLCRK
                     YYPK"
     gene            3418..4116
                     /gene="rfaY"
                     /locus_tag="QBH27_004537"
     CDS             3418..4116
                     /gene="rfaY"
                     /locus_tag="QBH27_004537"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDQ7016758.1"
                     /translation="MIQKSKIKDLVVFTDEENSKYLNVLNDFLSYNINIIKVFRSIDD
                     TKVMLIDTDYGKLILKVFSPKVKRNERFFKSLLKGDYYERLFVQTQKVRNEGLNTLND
                     FYLLAERKTLRFVHTYIMIIEYIDGIELCDMPDIDDSLKNKIQQSINALHKHGMVSGD
                     PHRGNFIIKNGEVRIIDLSGKRASAQRKAKDRIDLERHYGIKNEIKDLGYYLLVYRKK
                     MRNFMRRLKGKPAR"
     gene            4186..>5033
                     /gene="waaZ"
                     /locus_tag="QBH27_004538"
     CDS             4186..>5033
                     /gene="waaZ"
                     /locus_tag="QBH27_004538"
                     /EC_number="2.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-oct-2-ulosonate III transferase
                     WaaZ"
                     /protein_id="HDQ7016759.1"
                     /translation="MKNIRYIDKKDVENLIENKTSDDVIIFLSGPTSQKTPLSVLRTK
                     DIIAVNGSVQYLLSHNIVPFIYVLTDVRFLHQRRDDFYKFSQRSRYTIVNVDVYEHAS
                     KEDKLYILQNCLVLRSFYRREKGGFIKKIKFNILSQIHKELLISVPLSKKGRLVGFCK
                     DISFGYCSCHTIAFAAIQIAYSLKYARIICSGLDLTGNCSRFYDENNNPMPSELSKDL
                     FKILPFFRFMRDNVEDINIYNLSDDTAISYDVIPFIKFQDISREESKDKTRKKMQYRT
                     STDSYA"
BASE COUNT         1635 a          795 c          948 g         1655 t
ORIGIN      
        1 aaaattaatt actaataata taaatgtact tggttcggaa atctatagtg agtctcgctt
       61 ttttataata ggtcatgatg attgggataa gttagagtat tttattaatt catctgttaa
      121 accaatggat tatgatagtt tatataaatt cagccctgat aaaatgatgt caacaattgt
      181 ttatgatttt atagaaaaat aaaaccgtta aaacagtgag ttttactcaa ttaatgagag
      241 tttcaatgaa aatagcattt atcggcgaag ctgtttctgg atttggcggc atggaaacgg
      301 tcattcgtga tgttattact accttcaggc aacaacatat ctatagtgaa atgtttttct
      361 tttgtcgtaa tgataaaatg gataagggat ggctggaagg tattaagtac tcgtgttcat
      421 tttccaatat tcgcttaggt tttttgcgtc gggcgaaaca tattcatgct ttaagcaaat
      481 ggcttcaaga ttatcagcca gatgttgtta tttgtattga cgttatctcg tgtttgtatg
      541 cggccaaagc acgtaaaaaa tcaggaattg atgtgccagt attttcatgg ccgcattttt
      601 ctttagatca caaaaaacat gctgaatata tcacctgtgc agactatcat ttagccatca
      661 gctctggcat taaacagcaa atgattaatc ggggtgtacc agaatcgacg ataaatgtta
      721 tttttaatcc agtcgaagct aaagacacag tcattcctgg gcctgaggaa ggtgaaacgg
      781 caacgttcat ttatgttggg cgtatgaaat tcgagggcca gaagagggtt aaagacctgc
      841 ttgatggttt atcacaagcg caaggtgact ggaaactcca tgtactgggt gatggttctg
      901 attttgaaaa atgccaggca tatggtagag aattgaagat tgataatcga attgtctggc
      961 atggttggca aaaacatcca tgggaggtag tgcgtcagga tattaagaaa gtcagtgcat
     1021 tgttattaac ctcatctttt gaaggttttc caatgacctt acttgaatcg atgtcatggg
     1081 ggattccgtg tatcagtgcc gattgtgtct ccggtcctgg tgacattatt caacctgatg
     1141 tcaatggcca tttgtatcaa ccgggagata ttgctggttt tgttactctt ctaaataaat
     1201 atatagcagg tgaaattcat atcgaacatg aaaaaattcc tgcgtcgatc gataagtttt
     1261 atcaatctaa gtattatgat agattacata atataattat ttctgctatt tcccggagga
     1321 aataatgcag caggtatttt tccaggaaac tgagtatcta aattcagtta ttgattataa
     1381 tcataaagta gaaacagaaa atctttgtct ggacatcgct tatggaactg acaaaaactt
     1441 tctatttggt tgcggaattt cgattgcttc aatattaaag tataatgaag gaagtcggtt
     1501 gtgttttcac atttttactg attattttgg tgataatgat cgtaagtatt ttgatgccct
     1561 ggcgttacag tataaaacca gaattaaaat atatctgatt aatggtgatc gactgcgctc
     1621 attgcctagc acaaagaact ggactcatgc aatatatttt cgttttgtta ttgctgatta
     1681 ctttattagt aaggtggcta aagtccttta tctggatgca gatatcattt gtcaggggac
     1741 tattgagcca ctaattaaat tctcatttcc tgacgataaa gtcgcaatgg ttgttacaga
     1801 agggcaagct gactggtggg aaaaacgcgc ccattcgtta ggtgttgcgg gaatttctaa
     1861 aggttacttt aactccggtt ttttattgat taatactgcc aaatgggcag ctcagcaggt
     1921 ttctgcacga gccattgcaa tgctaaatga gccagaagta atcaaaaaaa taacacatcc
     1981 tgatcaggat gtattaaata tgttgctggc agataaactt attttcgctg atattaaata
     2041 taacacccag tttagcttaa attatcaact aaaagaaagc tttataaacc cagtaaccaa
     2101 caatactatt tttatccatt atatcgggcc aaccaagccc tggcatgatt gggcctggga
     2161 ttatcctgta tcacaagcct ttatggaggc aaaaaatgct tcgccatgga agaatacagc
     2221 gttgctgaaa ccgaacaata gcaatcaatt aagatatagc gcaaaacata tgcttaaaaa
     2281 acatagatat ctaaaaggat ttagcaacta tcttttttat tttattgaaa agataaagca
     2341 ttaaaactgg gaaacaataa agtaatgata taagggtagc attgtggact catttcctgc
     2401 catagagata gataaagtta aagcctggga ttttcggctg gctaatataa atacttctga
     2461 atgtttaaat gttgcctatg gtgtcgatgc taattatctt gatggtgtag gtgtttccat
     2521 tacatcaatt gtcctaaata atcgacatat taatcttgat ttttatatta ttgctgatgt
     2581 ttataatgat gcttttttcc aaaaagttgc aaagcttgca gagcaatacc aattaagaat
     2641 cacattatat agaattaaca ctgataagct ccagtgcttg ccttgtacac aggtctggtc
     2701 aagagcaatg tacttccgtt tatttgcatt tcaattattg ggtttaacac ttgatcgttt
     2761 gctttatctt gatgcagatg ttgtttgtaa aggcgatatt agccaactat tgcatctgga
     2821 tttaaacggc gcggttgctg ctgttgttaa agatgttgac ccaatgcagg aaaaggcggc
     2881 atccaggttg tctgatccag aattactcgg gcagtacttt aattccggtg tcgtttattt
     2941 agatttaaaa aaatgggcta acgcaaaact aacagaaaaa gcgttgtcta ttcttatgag
     3001 taaagataat gtgtataaat accctgatca agatgttatg aatgtcttac tgaaagggat
     3061 gacaatattt ttgcctcgtg gatataatac aatttataca attaaaagtg aattaaaaga
     3121 caaaacacat caaaactata aaaagctgat tatagaaaat actcttctta ttcattatac
     3181 aggtgcaact aaaccgtggc acaaatgggc aatttatcca tcgacaaaat actataaaat
     3241 agctttagac aattccccct ggaaagatga ctctccacga gatgcgaaat caattattga
     3301 attcaaaaaa agatataaac atcttttagt gcaacatcac tatatctcag gaattatagc
     3361 aggagtctgt tatctttgcc gaaaatatta ccctaaataa catttaactg gtttgttatg
     3421 attcagaaga gcaagatcaa agacttggtt gtttttaccg atgaagagaa ttcaaagtac
     3481 ctcaatgtat taaatgactt cttgtcttat aatataaata tcatcaaggt ttttcgttct
     3541 attgatgata caaaagttat gcttattgat accgattacg gtaaattgat tcttaaggtt
     3601 ttttctccga aagttaagcg taacgaacgt ttctttaagt ctctgttaaa aggtgattat
     3661 tacgaacgcc tttttgtgca aactcaaaaa gtacgaaatg aagggttaaa tacactcaat
     3721 gatttttatt tattggctga acggaaaacc ttacgatttg tccatactta tatcatgatc
     3781 atcgagtata ttgatggtat agagttatgt gatatgcccg atattgatga ttcgctaaaa
     3841 aataaaattc aacaatcaat taatgccttg cataaacatg gcatggtttc tggcgaccct
     3901 catcgtggta acttcattat aaaaaatggt gaggtccgaa ttattgatct ctctggaaaa
     3961 cgtgcttcag cgcagcgtaa agcgaaagat cgcattgact tagagcgtca ttacggtatt
     4021 aaaaatgaga ttaaagatct aggctattat cttttagtat atcgtaaaaa aatgcgcaat
     4081 tttatgcggc gtttgaaagg gaaaccagct cgctaaaaaa atcccacaat tacatgtctt
     4141 caccaggaca gttttaagcg gagttattta tttcacaggt ttatcatgaa gaatattaga
     4201 tatattgaca aaaaagatgt tgaaaacctg attgaaaata aaacatctga tgatgttatt
     4261 atttttcttt cgggccctac atcgcaaaaa acacctttgt cagtattacg aacaaaggat
     4321 attattgctg tcaatggttc tgtgcaatat ctgttaagtc ataatatcgt tccttttata
     4381 tatgtactta cagatgtccg ctttttacat cagcgtcgtg atgattttta taaatttagc
     4441 cagagaagcc gttataccat agtaaacgtt gacgtttatg aacatgcttc caaagaagat
     4501 aaactttata tcctccagaa ctgcctcgta ttacggtcct tttatcgccg ggaaaaaggt
     4561 ggttttataa agaagattaa atttaatata ttgagtcaga ttcacaaaga actgctgatc
     4621 tctgtaccgt tgtctaaaaa aggtcgtctg gttggatttt gcaaggacat tagttttggt
     4681 tattgttcat gccatactat tgcctttgct gcaattcaaa tagcatattc acttaagtat
     4741 gcacggatta tttgttctgg tcttgattta acgggtaact gctctcgttt ctatgacgaa
     4801 aataataatc ccatgccctc ggaattgagt aaggatttat tcaaaatctt gccatttttc
     4861 cgttttatgc gtgataatgt agaagatatt aatatctata atttatcaga tgatacagct
     4921 atttcttacg atgtaattcc atttattaaa tttcaagata tcagtagaga agaatcaaaa
     4981 gataagacaa gaaaaaaaat gcaatataga acttcaactg attcttatgc taa
//
LOCUS       DAOFSV010000231         5010 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.231, whole genome shotgun sequence.
ACCESSION   DAOFSV010000231 DAOFSV010000000
VERSION     DAOFSV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.231"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="QBH27_004539"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="QBH27_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDQ7016760.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="QBH27_004540"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="QBH27_004540"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDQ7016761.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LSNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="QBH27_004541"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="QBH27_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDQ7016762.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPKVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="QBH27_004542"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="QBH27_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDQ7016763.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
BASE COUNT         1323 a         1325 c         1284 g         1078 t
ORIGIN      
        1 tttttgcctg acctcatggg gggcaacggg gcgctactat agggagttgg aataaaacgg
       61 tcaagaagaa tttttatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttctaaagt actccataca tatcacaaaa
      181 taaaaaggcc ggctaaaccg accttttact cattctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgaggta
      781 cccagaaatt attcgacaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagtttacc gccatgacgg ataaattcat cacagaagac cagaagcgcc gttttggacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag tgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcgatg acctgaccaa aagcgtaatt catcgtgaca cgatagggtg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc cataacaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgccagg agaaaaccac gggaaaatac
     1321 cacgctgata agccattaat aggcgcgcag ggctaagatc gccccccagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gcccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc gcacatcatt tctgcaagca attcaaggat
     1681 ctgactttcg gttgtggcat ctaagccttc tgtaggttca tccagcaata ccagcggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgcca gagcctcatc actactacca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgggga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg actatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgac ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagataa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttt cggtttttga tccgttaaat cagtgatccg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagcgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggttag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta attgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagt ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc taccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagt aattcgccct gacgatagcg
     2881 cgccagtcct gcaggggaga ggggcagtaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcat cgtgacttac caggcgttca aaatagcgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggcggagag gaaccagccc gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcac tgacccaggc
     3601 gttatccagc gctgtttgta actcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattcc ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacactgct cgttggcctg ctggcaaagt aaaattcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ttcaatggtc accggatcgg tcgatgctaa
     3961 ttccgcttca ccgcgctgcg gatgggctag cggggtttcc ataaacgttt tcaaactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgaggta ggaaaaacca aagtagaccg ccaccagagc
     4201 aatcgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaat aacggaatta gcggtgcagt gcccagcaga atgagcgccg cggcccagtt
     4501 agaggggaag atagccacca caatcagcag cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaacagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaat tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga
//
LOCUS       DAOFSV010000232         4949 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.232, whole genome shotgun sequence.
ACCESSION   DAOFSV010000232 DAOFSV010000000
VERSION     DAOFSV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.232"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..658)
                     /gene="tonB"
                     /locus_tag="QBH27_004543"
     CDS             complement(<1..658)
                     /gene="tonB"
                     /locus_tag="QBH27_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDQ7016764.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     P"
     gene            882..1178
                     /locus_tag="QBH27_004544"
     CDS             882..1178
                     /locus_tag="QBH27_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDQ7016765.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1478..2731
                     /gene="kch"
                     /locus_tag="QBH27_004545"
     CDS             1478..2731
                     /gene="kch"
                     /locus_tag="QBH27_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDQ7016766.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILSKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(2786..2959)
                     /locus_tag="QBH27_004546"
     CDS             complement(2786..2959)
                     /locus_tag="QBH27_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDQ7016767.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            3102..4562
                     /gene="cls"
                     /locus_tag="QBH27_004547"
     CDS             3102..4562
                     /gene="cls"
                     /locus_tag="QBH27_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDQ7016768.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            4597..4926
                     /locus_tag="QBH27_004548"
     CDS             4597..4926
                     /locus_tag="QBH27_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDQ7016769.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
BASE COUNT         1215 a         1150 c         1195 g         1389 t
ORIGIN      
        1 ctggcttacc cggctcataa cgccatctgc gcatcgcatt tttcacttca cgctcaaaca
       61 tgttcgcagg cttggctgag aggatttgta cgttatccac gcgaccatct ggcgtgacat
      121 caaatttaac tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg
      181 gctgattacg gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg
      241 cagccgttgc cgtacttgat gtcgggcgcg cgggtgccgt attttcaaac ggtgatgccg
      301 gacgcgactc tacgggtttg acatcgcgtt ttggctgctc cacctttttc accggcttcg
      361 gttttggctt tggtttcggc ttcggctttt caatgaccac cggagcttct tttggcggtt
      421 cagggatcgg ctcaggttcc ggttctggct ctaccaccgg ctccggtggt ggttgaacgg
      481 cttgtggcgg ttcaagatca gcaggcgcaa ccatcgtgac agaaatcggc tgcgcaggcg
      541 caggtagttc aataacctga tgtaccgagg tatagagcaa acccgccaca acagcaccat
      601 gaatgcagac cgaaagtaac gtcggccagg ggaagcggcg aggtaaatca agggtcattg
      661 aagtcataat catttcagta gaaaaaccag gccccgattt taaatgcaaa tagcaatcat
      721 attcaataag gcaagacgat caggatcagt gaaaatgtca tttttggcgt aattgacctt
      781 actttatgca tggtcttaac aaagacatca tctttccgtt gcagatcttc gccctttcaa
      841 ataacgtact ttacaacttt cccgaacaag gagttgtgcc tgtgttgtat gttatttacg
      901 ctcaagacaa agctgatagc ctcgaaaaac gcctttccgt tcgtccggca catctagcac
      961 gtttacagtt actgcatgat gaaggtcgtt tgttgaccgc cggtccaatg ccagcggtag
     1021 acagtaacga tccaggggct gcgggattta ctggctcaac cgttattgct gaatttgagt
     1081 cgctggaagc agctcaggcc tgggccgatg ctgacccgta cgtagcggca ggagtctatg
     1141 aacacgtttc agtgaaacca tttaagaaag tgttctgaat agaaattcaa tatttaaggg
     1201 cacggttttt gcaaccgtgt tcttgtttgg cctttcctta taggtcggtc agtgcattca
     1261 ccaccacaag cagataataa ctttatctgt atttctttat atgatgtgaa catattcagg
     1321 aaaatgccct ttcctgacgc agagttgctc actcaaaacc tttgattact gcggataaag
     1381 tcatctacta ttgtttaaca cattcacgag ctgcatgtat gttcagaaaa tgtgcacgtt
     1441 gagataatta aacaaaattg tggttacaga ggtatcagtg agtcactgga ctacattcaa
     1501 acaaacagca acaaagttat gggtgaccct acggcacgac attctcgcgc tggccgtctt
     1561 tttaaatgga ttgcttattt ttaaaacaat ctatggtatg tcggtcaatt tgcttgatat
     1621 tttccatatc aaagcatttt cagagctgga tctctccttg ctggcaaacg ccccactatt
     1681 tatgctcggc gtatttcttg tcctgaactc cattggctta ctgttccggg caaagctcgc
     1741 atgggcaatc agtatcattt tgttgttgat agcgctaatt tacactctgc atttttatcc
     1801 ctggctgaaa tttagtattg gattttgcat ttttacgctg gtgtttttgc tgatactgag
     1861 caaagacttc tcccacagta gcgcagcagc cgggacaatt ttcgcattta ttagtttcac
     1921 gacgttactg ttttactcca cttacggtgc gctttattta agcgaaggtt ttaatccgcg
     1981 aatagaaagt ttgatgaccg cgttctattt ttcgatagaa accatgtcaa ccgtcggcta
     2041 tggcgatatt gtccctgttt ctgaatcagc acgattgttc actatttcgg tcattatttc
     2101 cggcattacc gtttttgcca catctatgac ctcaattttt ggtccgctta tccgcggggg
     2161 attcaacaaa cttgtaaaag gaaacaatca tacaatgcat cgtaaagatc attttattgt
     2221 ttgcggacat tcgattctcg ccatcaatac gattctgcaa ctgaatcaac gcggacaaaa
     2281 cgtaacggtt atcagcaact tgcctgaaga tgatatcaag caacttgagc aacgcttagg
     2341 cgataacgcc gatgttatcc ccggtgacag taatgacagt tcagtgttaa agaaagcggg
     2401 aatcgatcgt tgccgggcca ttctggcgct gagtgataac gatgcagata acgcgtttgt
     2461 tgtactctcg gcaaaagata tgagcagtga tgtcaaaaca gttcttgccg tcagtgatag
     2521 caaaaacctg aataagatta agatggtaca tccggatatc attctctcac cacaactgtt
     2581 tggcagcgaa attctggcgc gagttttaaa tggtgaagag attaataatg atatgctcgt
     2641 ttcaatgttg ttgaactccg gtcatggcat tttcagcgat aacgatgaac aagaaacgaa
     2701 agctgacagt aaagaatcgg cgcaaaaata gagtttaaag acggaagagt aaacttattg
     2761 ggcaccgcaa tggtgcccag tacatttaga tattgtagat cgcaaacaat aacgagttac
     2821 gctgtttgtt tagaatgcac ttcctgatgc tgtggatacg catatttcgg cgcgactgcc
     2881 cctcaagcca acgcgattta cgtcggctag cctgacgctg catgcgccag cgccccactt
     2941 ccgttctact ccgcttcatg ttaactactc tatgcaacaa cagaatggtc attatacccc
     3001 cgcccctgac gctgaccagt gcttttccct cgtttttatt tgccagatga atccatatgc
     3061 gtaaactcat aacaatgcgc tttcaaaagg atttctaatc tatgacaacc gtttatacgt
     3121 tggtgagttg gttggccatt ctgggatact ggttgctcat tgcaggcgta actttacgca
     3181 ttctaatgaa acgacgcgca gttccctccg cgatggcctg gctgttgatt atttacattc
     3241 tgccgttagt cggaattatt gcctatcttg ccgttggcga gctccattta ggcaaacgcc
     3301 gcgctgagcg cgccagagcg atgtggcctt ccaccgcaaa atggcttaac gaccttaaag
     3361 cctgtaagca tatcttcgcc gaagaaaata gcagtgtcgc tgcgccatta ttcaagcttt
     3421 gcgagcgtcg tcaggggatc gctggggtca aagggaatca gctacaactg atgaccgagt
     3481 cagatgatgt gatgcaggcg ttaatccgcg acatccagct cgcgcgccat aatattgaga
     3541 tggtgtttta tatctggcag cccggcggca tggcggacca ggtggctgaa tcattaatgg
     3601 ccgctgcacg gcgcggtatt cattgccgat tgatgctcga ctccgccggg agtgtggctt
     3661 ttttccgcag cccgtggccc gagctaatgc gtaatgccgg tattgaagtt gtcgaagcct
     3721 taaaggtcaa tctgatgcgt gtgtttttac gccgtatgga cctgcgccaa catcgcaaga
     3781 tgatcatgat cgataattac atcgcgtaca ccggcagcat gaatatggtc gaccctcgct
     3841 acttcaaaca agatgcgggt gtagggcaat ggattgatct gatggcgcgt atggaaggcc
     3901 ccatcgccac cgcgatgggg attatttatt cctgcgactg ggagattgaa accgggaaac
     3961 gtattctgcc gccgccacca gatgtcaata ttatgccgtt tgagcaggcc agtggtcaca
     4021 ccattcacac aattgcttct ggccccggct ttccggaaga tctcattcac caggcattat
     4081 tgactgctgc ttattcggct cgtgaatatt tgatcatgac aacgccctat tttgtgccaa
     4141 gcgatgattt acttcatgcg atttgcacgg cggcgcagcg cggggtggat gtcagtatta
     4201 tccttccgcg aaaaaatgac tcgatgctgg tcggctgggc cagtcgcgca ttctttacag
     4261 aactgctggc tgctggggtt aaaatttatc agtttgaagg cgggttactg cataccaaga
     4321 gcgtgctggt cgatggagaa ctaagtctgg ttggcaccgt taaccttgat atgcgtagtc
     4381 tgtggctaaa tttcgagatt accctggcaa tcgacgataa aggttttggt gcagacctcg
     4441 ccgccgttca ggacgattat atttcgcgtt ctcgtctgct cgatgcccgt ttatggctaa
     4501 aacgtccatt atggcaacgt gtcgccgagc gactgtttta cttcttcagc ccgttgctgt
     4561 aaaacgtcgc caacagaggt taaacaggta gtgactatgg atatggatct aaacaatcgc
     4621 ctgactgaag atgaaacgct tgagcaggcc tacgatattt ttctcgaact ggctgccgac
     4681 aatctcgatc cagctgatgt tctgctgttc aatcttcagt ttgaagagcg cggcggcgcg
     4741 gagttattcg atccggcgga agactggcag gaacatgttg atttcgattt gaacccggac
     4801 ttttttgccg aagtggtgat tggtctggcg gacagtgaag atggtgaaat caacgatgtt
     4861 ttcgcgcgca ttctgttatg ccgcgaaaaa gatcacaaac tttgccatat catctggcgg
     4921 gaataaataa tcgcttttgg cgtgaataa
//
LOCUS       DAOFSV010000233         4860 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.233, whole genome shotgun sequence.
ACCESSION   DAOFSV010000233 DAOFSV010000000
VERSION     DAOFSV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.233"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..625
                     /locus_tag="QBH27_004549"
     CDS             <1..625
                     /locus_tag="QBH27_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7016770.1"
                     /translation="ALLMLLRLPGTPPQPTPECKSVSLKRDCLPYLLCAILLAAAVSM
                     MQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGVLRPQRLTPVALLLSAG
                     VLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLNDKLADGAGAGWLATSHT
                     LGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWHYRTIK"
     gene            987..1200
                     /locus_tag="QBH27_004550"
                     /pseudo
     CDS             987..1200
                     /locus_tag="QBH27_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(1343..1771)
                     /locus_tag="QBH27_004551"
     CDS             complement(1343..1771)
                     /locus_tag="QBH27_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HDQ7016771.1"
                     /translation="MKKFINVYIKSDYDIVILRLSVIIIFMLFGTYKWFDFEVMALKP
                     LISPTWLNILYIMFGVHGGSYFLGIVETITFLALSIGFFRPAAGILGDIIVIVTGLVT
                     LSLLPQLGKVDSFVIKDVLLLGAGSVLLKYDLKNYLNIKN"
     gene            complement(1811..2371)
                     /locus_tag="QBH27_004552"
     CDS             complement(1811..2371)
                     /locus_tag="QBH27_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ7016772.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDIYSGTVNALIKMLDTRT"
     gene            complement(2413..2673)
                     /locus_tag="QBH27_004553"
     CDS             complement(2413..2673)
                     /locus_tag="QBH27_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7016773.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            3222..3971
                     /locus_tag="QBH27_004554"
                     /pseudo
     CDS             3222..3971
                     /locus_tag="QBH27_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            4507..4620
                     /locus_tag="QBH27_004555"
     CDS             4507..4620
                     /locus_tag="QBH27_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDQ7016774.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
     gene            4711..>4860
                     /locus_tag="QBH27_004556"
     CDS             4711..>4860
                     /locus_tag="QBH27_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7016775.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVW"
BASE COUNT         1329 a         1049 c         1174 g         1308 t
ORIGIN      
        1 ggcgctgctg atgctgctgc ggttgcccgg cacaccacca cagcccacac cggagtgcaa
       61 gagcgtcagt ctgaagcggg attgtctgcc ttatctgctt tgcgcaatat tactggctgc
      121 ggcggtgagc atgatgcagc ttggactttc gcctgccctt actcgccagt tcgccacgga
      181 taccaccgcc attagccaac aggtggcctg gctgttgggt ctgtccgcag tagctgcgct
      241 tatcgcgcag ttcggggtac tccgtccgca gcgcctgact ccggtggccc tgctcctgag
      301 tgccggagtg ttgatgagtg gtggtctggc tatcatgctc tccgaacagc tatggttgtt
      361 ttacccgggc tgtgcagtgc tgtcatttgg agcagctctg gcaacacccg cttatcaact
      421 tctactgaat gataagctgg ctgatggcgc aggcgcgggc tggctcgcta ccagtcacac
      481 acttggctat ggactgtgcg ctttgctggt gccgctggta tcgaaaacag gtgtcgcaat
      541 cgcgctgatt atggcagcat tatttgcaac tatattattt accattgtgt ctgtatttat
      601 ctggcattac cgtactatca aataaagata tgatcatcac cttactggag taaatcagag
      661 gatatgagag aggacctgcg tctgatgaaa acatgtcagg cagtgttctg gcttgctgcc
      721 cccgcctggt ttacgttacg cccttagcta cctggctaaa caggatcaga aagaacacgg
      781 tattattctg atgcgtagca gacttcccga aaaaagtaac cgcggccgac cacgacacaa
      841 ctgaattaca gctgtattag aattaagttt ccgtaagttg ttgggaggtg cttgagttaa
      901 taggcagcag agggggcgct gtaatcagtg taggggaccc gaatttctgc tgtggtgctt
      961 cagtggttcg gatacaggta acgaatatgg ttcataaatc tgacagtgat gaattagcag
     1021 cactgaggga agacatcact tgaagcttat gggattgaat ggcgacgtaa accgcagagt
     1081 cctgtgcagt gcgtttccgt attatccacg gatgagaaag ttgcgttatt tcgtcggttg
     1141 tttcgtgggc gtagtgatgt atggggcctt cgatgggaaa gtaaaactag cggaaaataa
     1201 aagtactcgc ctgcctacgg acctttttac tcagggtatt tttgttttcc ataacgttgc
     1261 gtattaacat gaaggagaac gagggggatt aggtaatctc tttccatgaa aatacgcaaa
     1321 cgttcatcac cgaagtgaaa aatcagtttt tgatatttaa ataatttttc aaatcatatt
     1381 tcagcaggac actaccagca cctaacagca gcacatcttt gatgacaaaa ctgtcaactt
     1441 ttcccagttg tggtaacaga cttagtgtga ctaaacctgt aacaataacg ataatatctc
     1501 ccagtatgcc agcagcaggc ctgaagaagc cgatactcag tgctagaaaa gtgatagttt
     1561 ctactatgcc gaggaaatag ctccccccat gaacaccaaa cataatgtac aggatattca
     1621 gccacgtggg agaaatcagg ggcttgaggg ccatcacttc aaaatcaaac catttatagg
     1681 tcccgaagag cataaatatt ataataacag acaggcgaag aataactatg tcataatctg
     1741 atttaatgta aacattaata aattttttca tttcattctc cgggtggaaa attatatgca
     1801 ctaaaaagga ttaagtacgg gtatcaagca ttttaattaa agcgttaacg gttcctgaat
     1861 agatatcatg tggtggaagt cgatttaaca tcagtgcccc ctttagtgta gaacagacta
     1921 taacggcgag ctcatccgga agaagaactg ttttgaactc tccactattc tgcccggagg
     1981 tgataatatc tttaatcatc tgtatctcct gatgtgccag ttgacttaca gcctcctgta
     2041 attcaggaga aaactgatgg ctgtcggata gcatggcata aataccgcac atttcccctc
     2101 tttcagcaca accagcaaat gcatcaagat atgtcctgag acgttgtaca cctgaagaaa
     2161 cattatggag agttatatta agtctatcaa atacttctgt tttgtattgg cagtaggcta
     2221 taccaagatc agttttcctc gggaaatggt gatgaatact ggcttttctg ataccaattt
     2281 ctttttccag atctgcgtaa ctgaacccca aaaatccgcg ttgctggatc agagtatccg
     2341 ttatgcgaag tatattctca taagtagaca cggctttacc tgttatgtat taaagggact
     2401 attatacata ttttagcgat agataatggc agttccatca agtttatttt ttcctcccaa
     2461 tgaagtgatt cggaaatgag aacctcctgc ctgctccgct ttgtctttaa gcgcagttgc
     2521 cacctcttct ggtgtatata gattaccaac agaaatatag tctgacggca tcaaaccaac
     2581 aacgtcctga cgattaattt catctgcagc agttgcgtag cctgtcagaa tgagaccaag
     2641 ggcgacaatg gtagtgcaga taacataatt catgagcaat ttctctttat gtttgcgata
     2701 ctacacatgc taccttctga ttggtaggct tgcaagatgg atttatttaa aataagaggg
     2761 atgatttgta tcaaattgtt ttgtattttt gtgttttgta tcactgaggt gaagttgtaa
     2821 gatctggccc tctgcgaggt tggctctgtc gaaaggaaca ggtgacgaga gttaacatcg
     2881 ttgccttggc tgcatggggg atatgtagct accccctgat atttccgcca cggaagccaa
     2941 tatgaacaac aataacaata cgcctcgttt ccagtatgct tcatgtcgga tattttgtaa
     3001 gcgctatgta cttcccggga cgcttatctt ttagccgtag ctggtagtgc aataaaagcc
     3061 tggcagcaat ccaggcctct acgttctaag aatcaactgt cagtgcagag gaatgcgcac
     3121 gacgctctcg ttgttattgg cttttgccgt cgcaccgtcg tttttcacgg tgacaaacag
     3181 cgcgtgcgct ttgctatccg tcttatatca ctggtgctga caatgtggat gaggtcgcct
     3241 ttaaaaaagg acatcgttat ataacggtga tctccgatcg cgatggtcgt gcgctggcct
     3301 taacggacga tcgtggcaca gagagcctcg ccggctatct tcgcacgctt actgacgggc
     3361 agttgctggc tatcaaaacg ctctcaatgg acatgaatgc aggctatata agagcagcgc
     3421 gtatccactt accctgtgcg gttgagaaaa tcgccttcga ccgcttccat gtagcaaagc
     3481 aacttggcga agtggttgat aaaacccgtc agaatgaaca tccgcacctc cctgttgaaa
     3541 gttggcgtca ggcaaagggg acccgcttcc tgtggcagta cagcgataaa tggatgacca
     3601 aatccaggca ggaaaagctg atatggctgc gtgcacagat gaagctgaca agccagtgct
     3661 gggcgctgaa agagctggca aaggatatct ggaacaggcc atggagtgag gaaagacgta
     3721 atgactggga gagatggctg gctctggcgg ccaacagtga cgttcctatg atgaaaaacg
     3781 ccgcgaaaac gataggaaaa aggctttacg ggatcctgaa tgccatgcga caccgtgtct
     3841 caaacggaaa cgcggaggcg cttaacagta agatcaggct actgaggata aaagccaggg
     3901 gataccgaaa tcgggagcgc tttaagctgg gcgtgatgtt ccactacggg aaactgaata
     3961 tggcgttctg agccttccca ccatgatcag ggaaaaccca tgtatcatca ggttattatg
     4021 tgagctggta aatcagattt tgcatggact gaaatcatgc tgttatttag ggataaagaa
     4081 ccggcttttt gtcccgcccc ctcgccatta atgaaaaaaa gaatggaatg ctccctctga
     4141 ggagaagggc agtagaaaaa agaacccgcc agcaaaaata ttggggatga acagctttcg
     4201 ctactcagat tgctggcggg tatagttcct catgaaataa aaatactaca taatcctttt
     4261 aatgcaaatg ataatcattg tcaattgttt gtgcgtgttt tcttcataca tgattggtag
     4321 taaaggtgat tcaggctatc agtttttgct gatgatcttt ttatctatag aaaacatcag
     4381 ccaagctggt tggttcagta acgctttcat ctttgagcat tttataaaaa attcttgaat
     4441 tgttaaaata gattaaagtc tggtaacagc aaaagttaaa cgagaaatca aaaggaggtc
     4501 ccaaatctga aaatgaagca tctgcgtgat gatgttgcag cgggacgtcg tcggctgtat
     4561 atcagagcaa tctgtccatc cctgcgtgaa gccccgttac tttattcgta ccccttataa
     4621 tggggtgtta gccagccaga cccggcatga ttactgtgaa ccgccccggg aatcctggag
     4681 actaaattcc ctgagaaaga ggtaaacagg atgactaaaa atacacgttt ttcccccgaa
     4741 gtccgtcaac gggcagttcg tatggttctg gaaagtcagg acgaatatga ctcacaatgg
     4801 gcggcaattt gttccatagc tccaaagatt ggctgtacgc cagagactct gcgtgtctgg
//
LOCUS       DAOFSV010000234         4843 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.234, whole genome shotgun sequence.
ACCESSION   DAOFSV010000234 DAOFSV010000000
VERSION     DAOFSV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.234"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            137..967
                     /locus_tag="QBH27_004557"
     CDS             137..967
                     /locus_tag="QBH27_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDQ7016776.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGVWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            1025..2152
                     /gene="efeO"
                     /locus_tag="QBH27_004558"
     CDS             1025..2152
                     /gene="efeO"
                     /locus_tag="QBH27_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDQ7016777.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            2158..3429
                     /gene="efeB"
                     /locus_tag="QBH27_004559"
     CDS             2158..3429
                     /gene="efeB"
                     /locus_tag="QBH27_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDQ7016778.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            4056..>4843
                     /gene="phoH"
                     /locus_tag="QBH27_004560"
     CDS             4056..>4843
                     /gene="phoH"
                     /locus_tag="QBH27_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDQ7016779.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT         1165 a         1150 c         1345 g         1183 t
ORIGIN      
        1 aaaatatttt tacatcttct catcattatc actacatttc acttgtattt ttcttcatcg
       61 taatgataat cgctatcact gcgatttact tttctttgca tagattgact aagaaaaacg
      121 tttaagggtg ggtggcatgt ttgttccgtt tctcattatg ttgcgcgaag gacttgaagc
      181 cgcgctgatt gtcagtttga ttgccagcta tcttaagcgt acccagcgag gccgatggat
      241 tggtgtaatg tggattggcg tgttgcttgc cgctgcgttg tgcctgggct tgggtatctt
      301 cattaacgaa accaccggcg aatttccgca aaaagaacag gaactgtttg aaggcattgt
      361 ggcggtgatc gccgtggtga tccttacctg gatggttttc tggatgcgca aagtgtcgcg
      421 caacgtcaaa gtacaactgg agcaggcggt cgatagcgca ttgcagcgtg gaaatcatca
      481 tggctgggcg ctggtgatga tggtcttttt tgccgttgca agggaagggc tggagtcggt
      541 ctttttcctg ctggcggcat ttcaacaaga tgtcggggtc tggccgccgc tgggtgcaat
      601 gctcggtctt gctacagccg tggtgcttgg cttcctgctc tactggggcg gtattcgcct
      661 caatcttggt gcatttttta aatggaccag tctgtttatt ctcttcgtcg ccgcagggct
      721 ggcagctggt gccattcgcg catttcatga agccggattg tggaaccact ttcaggaaat
      781 cgccttcgat atgagcgcgg tgctctcaac tcactcgctg tttggcacac tgatggaagg
      841 gatttttggc tatcaggaag caccgagcgt cagcgaagtc gccgtctggt ttatttatct
      901 catcccggcg ctggtggcat ttgttctgcc accacgcgca ggggcgactg cgtcccgctc
      961 cgtgtagcaa atacgacgca aactcttgct tagttacaac atactttaaa gggatagtct
     1021 cgtcatgacc attaacttcc gtcgtaacgc attgcagttg agcgtggctg cgctgttctc
     1081 ttctgctttt atggctaacg ccgccgatat tccgcaagtc aaagtcactg tgacagataa
     1141 gcagtgcgag ccgatgacca ttacggttaa cgccgggaaa acacagttca ttattcagaa
     1201 ccacagccag aaggcgctgg agtgggagat cctcaaaggc gtgatggtgg tggaagagcg
     1261 ggaaaatatc gcccctggtt ttagccagaa aatgacggcg aatttacagc ctggcgaata
     1321 cgatatgacc tgcggtctgc tgactaaccc gaaagggaag ttgatcgtca aaggtgaagc
     1381 aacggcggat gcggcgcaaa gtgatgcgct gttaagtctt ggtggtgcaa ttactgcata
     1441 taaagcgtat gtcatggcgg aaaccacgca actggtgacc gacaccaaag cctttaccga
     1501 cgctattaaa gcaggcgata tcgaaaaagc gaaagcactg tatgcgccga cgcggcagca
     1561 ctatgagcgc atcgaaccga ttgctgaact gttctccgat ctggatggca gcattgacgc
     1621 ccgtgaagat gattacgagc aaaaagccgc cgatccaaaa ttcaccggtt tccaccgtct
     1681 ggaaaaagca ttgtttggcg ataacaccac caaagggatg gataagtatg ctgaccagct
     1741 ttataccgat gtggtcgatt tgcaaaaacg catcagtgaa ctggctttcc ctccttcaaa
     1801 agtggtcggc ggtgcagccg gactgattga ggaagtggcg gccagcaaaa tcagcggtga
     1861 agaagatcgc tacagccaca ccgatctgtg ggatttccag gctaacgttg aaggctcgca
     1921 gaaaattgtc gatctgctgc gtccacaact acaaaaagcg aatccggaac tgttggcaaa
     1981 agtcgatgcc aacttcaaaa aggtcgatac cattctggcg aaataccgca ctaaagaggg
     2041 ttttgaaaat tacgacaaac tgaccgatgc cgaccggaac gcactgaaag gaccgattac
     2101 tgcgctggcg gaagatctgg cgcaacttcg tggtgtgctg gggctggact aagcgttatg
     2161 cagtatgaag atgaaaacgg cgtgaatgaa ccgtcacgcc gacgtttact gaaagggatt
     2221 ggtgcgctgg cgctggcggg aagttgtccg gttgctcatg cacaaaaaac gcaaagtgcg
     2281 ccgggtacgc tttcaccgga tgcacgcaat gagaaacagc cgttttatgg tgagcatcag
     2341 gcagggatcc tgacaccaca acaggctgca atgatgctgg tggcgtttga tgtgcttgcc
     2401 agcgataaag ccgatcttga acggttgttt cgcttgttga ctcagcgttt tgcttttctg
     2461 actcagggcg gagcggcacc agaaacgcca aatccgcgcc tgccaccact cgattccggc
     2521 attcttggcg gctacattgc gcccgataat ctcaccatca cgttatcggt gggtcactca
     2581 ttgtttgatg agcgctttgg ccttgcgcca cagatgccaa aaaagttgca gaagatgacg
     2641 cgtttcccca acgactcgct ggatgcggcg ttatgtcatg gtgatgtgtt gctacaaatt
     2701 tgcgccaaca cccaggacac ggtgatccat gcgctgcgcg atatcatcaa acacacgccg
     2761 gatttgctca gcgtgcgctg gaagcgggaa gggtttattt ccgaccacgc agcgcgtagt
     2821 aaaggcaaag agacgccgat aaatttgctg ggctttaaag acggcaccgc caatcccgat
     2881 agccagaatg cgaagttgat gcaaaaagtg gtgtgggtga cggcagatca gcaggagcct
     2941 gcgtggacaa tcggtggcag ctatcaggca gtacgcttga ttcagtttcg agtggaattt
     3001 tgggacagaa cgccgctgaa agagcagcag acgatatttg gtcgtgataa gcaaaccggt
     3061 gcgccgctgg gcatgcagca tgagcatgat gttcccgatt acgctagcga tccggaaggg
     3121 aaggtgattg cgctggacag ccatatccgg ctggcgaatc cccgcacggc ggagagcgag
     3181 tccagcctga tgctgcgtcg tggctacagt tattcgttgg gcgtcaccaa ttccgggcaa
     3241 ctcgatatgg ggctactgtt tgtctgctac caacacgatc tggaaaaggg cttcctgaca
     3301 gtacaaaaaa ggttgaatgg cgaggcgctg gaggaatatg tcaaacctat cggtggcggc
     3361 tatttctttg cgctgccggg tgtgaaggac gcgaacgatt atctcggaag cgcattattg
     3421 cgggtttaat gtttttaggc gtataaggcg tttacgccgc accaggcgtt cgtgcgctga
     3481 tgcgacgctt gcgcgtctta tcatgcctac aatcggtgcg ggtcggtagg ccggataagg
     3541 cgttcacgcc gcatccggca agcttttacc agtaccgcga atcctgctga aagcacacag
     3601 cttttttcat cactgtcatc actctgtcat ctttccagta gaaactaatg tcactgaaat
     3661 ggtgttttat ggttaaatat aagtgaatat attgttgcaa tgaatgcgaa atctgttgta
     3721 cttattaagt agcagcggaa gttcccggca gtgatagtca gtcactatgg agatcgcgga
     3781 tggtaacgtc ctgtactgga catgttttag agaatcagcg cgccaccact cgcggagatt
     3841 tctcctccgg tagtcatctc gtcactttgt attttcaacc tcatcctttc ttttcatgtg
     3901 ttaccgacgc cgtaaacggt gcgcgtagcc gtttttcggc attttatcca aaagcaaact
     3961 atggcttaca aggaagccaa ccctctgatg ttcgtgcgca taatcgcgct gccaacggcg
     4021 cgtgtgatga atacaaacaa ctcaaggtgc tctccatggg aagacaaaaa gcagtgatca
     4081 aagctcgtcg cgaggcaaaa cgtgtgctga gacgggattc gcgcagccat aagcagcgtg
     4141 aagaagaatc ggtcacctcg cttgtgcaaa tgagcggcgt agaagccatt ggtatggccc
     4201 gcgacagtcg cgatacctcg cccatcctcg cgcgaaatga agcgcaattg cactatctga
     4261 aggctattga gagtaagcag ctgatatttg ccacgggtga agccgggtgc ggaaaaacct
     4321 ggatcagcgc agcaaaagcg gcagaggccc tgatacataa ggatgttgac aggattatcg
     4381 tcacccgtcc agttctgcaa gccgatgaag atcttggttt cttacctggt gatatcgcag
     4441 aaaagtttgc tccctatttt cgcccggtct acgacgtgct ggtccgtcgc ttaggggctt
     4501 cctttatgca gtactgcctg cgaccggaaa tagggaaggt ggaaattgcg ccgttcgcct
     4561 atatgcgtgg acgtaccttt gaaaatgcag tcgtcattct tgacgaggcg cagaatgtga
     4621 ctgccgcgca aatgaaaatg tttttaaccc gcctcgggga gaacgtgacg gttatcgtca
     4681 acggtgatat cacgcaatgc gatttgcctc gcggcgtgcg ctccggatta agtgacgcgc
     4741 tggaacgttt cgaagaggat gaaatggtcg ggatcgtccg attcggtaaa gaggattgcg
     4801 tacgttcggc actttgccaa cgtacgctac atgcatacag tta
//
LOCUS       DAOFSV010000235         4834 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.235, whole genome shotgun sequence.
ACCESSION   DAOFSV010000235 DAOFSV010000000
VERSION     DAOFSV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.235"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(106..981)
                     /gene="rluB"
                     /locus_tag="QBH27_004561"
     CDS             complement(106..981)
                     /gene="rluB"
                     /locus_tag="QBH27_004561"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDQ7016780.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1194..3089)
                     /locus_tag="QBH27_004562"
     CDS             complement(1194..3089)
                     /locus_tag="QBH27_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDQ7016781.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGISEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGDEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEAHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGEARKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(3117..3737)
                     /locus_tag="QBH27_004563"
     CDS             complement(3117..3737)
                     /locus_tag="QBH27_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDQ7016782.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(3734..4615)
                     /gene="yciV"
                     /locus_tag="QBH27_004564"
     CDS             complement(3734..4615)
                     /gene="yciV"
                     /locus_tag="QBH27_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDQ7016783.1"
                     /translation="MSDTNYSVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQQLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            4753..4797
                     /gene="trpL"
                     /locus_tag="QBH27_004565"
     CDS             4753..4797
                     /gene="trpL"
                     /locus_tag="QBH27_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDQ7016784.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    4774..>4834
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
BASE COUNT         1274 a         1280 c         1097 g         1183 t
ORIGIN      
        1 ttattcataa gcaagtatga aattaaatta aaatactgcg actatttatc atacttcaaa
       61 tgaagaaaag gccccgcagg gcctttattt aatacttaac ttaaattaac cgttattatt
      121 acgtccgcca gaacgacgac cgccgctcac ctgactgtga cgtttcaccg cacgacgaat
      181 ctgattcgcc ttcatacgac gacggtcttt ttctacagcg actttagagc tggtttctgg
      241 cggtagttcc accagttcgc gcagatagtt agtctgggcg aggtccagct ccgtccagcc
      301 accgcgtggc agaccttttg gcagtgggat atcaccgtaa cgaacgcgaa tcaggcggct
      361 cacctgcaca ccaaccgctt cccacagacg acgaacttca cggttacggc cttcggtcag
      421 agtaacgttg taccactggt tgatcccttc gccgccgctg aacttgatgg ttttaaaggc
      481 tgccggacca tcttccagct gcacgccacg actcaaatca cgcagtttcg cgtcgtcaac
      541 ctgaccaaat acacgcacgg catattcacg ttcaacttca cggcttggat gcattaaacg
      601 gtttgccagt tcaccgtcgg tggtgaacaa caacagacca caggtgttaa cgtccagacg
      661 acctacggca atccagcgtg cgccacgcag ctttggcaga cggtcaaaca ccgttggacg
      721 cccttccgga tcgttacggg tacacaactc accttccggt ttgtaatagg ccagaacgcg
      781 acaaatttgt tcagcggact cacgtaccga aatcaggtga ccatcgatac ggattttcag
      841 tccaggggta atttcaacgc gatcgccgag tttagcaatt ttgccatcaa cactcacacg
      901 accggcttca ataatagatt cgatttcacg gcgagaaccg tggccggcac gcgccagcac
      961 tttctgtagc ttttcgctca tagagcttcc ttttggtgtc gccttcccag gcgtctgata
     1021 tgcttttgtg caacaatgag tcagaactta catctgactc actgatagta aagtctgttt
     1081 gaactcttca ggccttaagt aaacgactcg acgttttaac tggcctggct ttgaggcgca
     1141 acatactaca ctatctttat ggttaaagct tgtttcgatt aacatcagct ttattaccag
     1201 ccaccaccgc ctccgccacc cgcaccgccg ccagcaccac caccggaata acttcgcccc
     1261 aacaaactgg tattggcccc cagactcaga tgcatctgta atgagaacaa aacgccacta
     1321 cgggcaattt ccggggcgcg gacagccgct gtgagtgatg gggtgatacg tgcagcccat
     1381 tctctgccaa ggtttgccga gaccgcccaa cctaacaaag cgtaatccag tttccgtgct
     1441 tcccctttcc tccgccgccc actgtgtgtt gctgcctctt tacgcgcaag atagttaacg
     1501 atagcttcac cgtgggcata acgttgctgc ccagtttggg taaactttgg cagaaaaata
     1561 taccccatgc cagtgagata accggtcagg aaaacagcgc taaaataacc cgctggcata
     1621 taccagtaaa agaatacatc tccgacattg atgtagagag aatagaaggc cacgcctgaa
     1681 caaatcagtg gtaaaaacaa agtgatgatt acacgattaa cgcaaggctt gtcatcatcg
     1741 cgggcaaaca aaagctcaag ggaacaaaat aagaaaggaa gaagccagca gatcataata
     1801 aacatatcgc ctaccagact cattcctgcc caacctacgg gactcaggtt accgtagaga
     1861 atgaccgcca atatcgccat cccgccccat tgcaaaaacg catcaggtcg gtaaaacgaa
     1921 gactttttac gcggcagata aaatttctgc atccgtaaaa atgccttgcg taagactctg
     1981 ttgcgtctcc ccgtaagaac gaccttatcg cctttacgaa ataacgcttc cagtaataac
     2041 tgctctgcgg gcagtaatac cgcattattt ctggtcgcgc cttcattaac acgaatcagg
     2101 atttgctggt tcttgtcata atgagcttcc agatgaattt ttcctttcac aatcagattt
     2161 acgatatcgg cgcaaaaacc tttatcgtca taaaccagct tcgcatcgag acgtaacatg
     2221 ccgggcgtgt aatctggcgg aatgacatcg gtttcaatca catctaccgg cgtaaattgc
     2281 ggcctgcgct tccagagata taaccatcca cacgccagga acaggcacgg aaaccaaatt
     2341 aacaacgatg acgtagaggg taaaagaaga tggctgaaaa tattcgtcgt ttgtggtgcc
     2401 ggggcattac caagtaaagc gtgaggccag cggtagagga ccgtgaaatc ttcacgataa
     2461 aacggatcgc gagattcaat tcttccgtcc tcaaggatgc ggccatttcg atatgtgtcg
     2521 ccctcatctc cggtaaagag atcgatttcg ctaaatggat tacccgcaat atcaggcaac
     2581 ttgagtgaaa atagggtctt atatatttca aacggccagt ggttaccggt cacgttccag
     2641 attagcagat cagaatcccc ctcacgcagg aaagcatttt taacctgata atgaatttga
     2701 taatgatagt cgccttgagg caagaaacgt tgtttatcgc ccacgacaat actcattaac
     2761 ccgatatgcc agtcaagatg ccagggttca gaaataccat cacgggtgac gccaacaata
     2821 tgatagttca gcagtgcagc gtccccatca ggctgcatcc agagtcgcgg taaatcacga
     2881 taaaatccgt gcttaatctg ctgatttcgc gcctgaaaat gaatgttctc tttgatgtcg
     2941 agcaccccat tcggttgcag agtggcttca acatcaaaga gcgagatccc ttcttcataa
     3001 gaagggatct ccgtgttttt cgcataactc aacgaagaga aaaaaagccc aactatcaat
     3061 agcaaaatgc agcgatatat ccctgccata atcttccatc cgtggaacgc aatttgttat
     3121 aagaaaggct tcacatcacc tacgccttca cgcaccacga ccggcgtatc atcggtgaga
     3181 tcaataaccg ttgttggttt ctgcccgaga tagccgccgt gaataatcaa atccacctgt
     3241 ttttccagac ggtctttaat ttcttccgga tcggattcgg taaattcact gcctggcagc
     3301 attagcgaag tggaaagcat cggttcgccc aatgcttcaa gcagcgcctg cgcaatcggg
     3361 ttagacggca cgcgcatacc gatggttttg cgtttttcct gcaacagacg gcgtggcacc
     3421 tccttagtcc ccttcaggat gaaggtatag ttgcctggcg tgttgttttt catcaaacga
     3481 aacgcaacgt tatcgacaaa tgaataggtc gagagttcag aaagatcgcg acacatcagg
     3541 gtaaagttgt gaccgtccgg cagctggcga atacgacaaa tacgctccat cgcgttttta
     3601 tcttcaattt tacagccgag cgcatagccg gaatcagttg gataaacaat caccccgcct
     3661 ttacgcacga tctccaccgc ctggttgatc aggcgttgct gcgggttatc aggatgaata
     3721 taaaaaaact ggctcataat tccctctctg tggtgttctg cggctgttcc catagctgcc
     3781 agacgccttc aacgcctgcg ggcagccaga gtttacgacc cagttcgatc cacgggcatg
     3841 gttgatgaaa atcagatcct tgtgatgccc ataaatgatg ctgacgcgca agtgtcgcca
     3901 gctgggtgcg ttcattgggc gattgctggc actgcgcgac ttccatcgcg tcgccgtggt
     3961 gttcggcaaa atgcgcaacc aatcttttca gccatttagc actaagattg taccgccctg
     4021 gatgagctag caccgcctta ccgccagaat gatgaatgac atcaatagct tgttctattg
     4081 tacaccactg tggcggaacg tatccggttt tcccgcgcgc cagatacttt ttaaagacat
     4141 ccgccattga actggctttt ccgcactcca caaggaaacg cgcaaaatga ccgcgcgtca
     4201 ccgcaccgcc ctgcgccagt tgttgcgcgc cttccagtgc cccaggtatt tgtgcttttt
     4261 caagtctttc ggcaatcagc tgcgcccgct gattgcgccg ttctgtctgc tgcgcgagga
     4321 actcacacat tagcggatga gtaatatcaa tattcagccc gacaatatga atttcatgat
     4381 tttcccagac cgtggaaatt tccacaccag gaataagatt cagcgccagt cccgaacgtg
     4441 aaatttcctc tcttgctggt gcaattgcag ctgtggtgtc atggtcggtg atcgccaggg
     4501 tgccgacgcg catctcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
     4561 aagctgtggt atggctgtgc aggtcgtaaa tcactgagta attcgtgtcg ctcaaggcgc
     4621 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg
     4681 aaatgagctg ttgacaatta atcatcgaac tagttaacta gtacgcaagt tcacgtaaaa
     4741 agggtatcga caatgaaagc aattttcgta ctgaaaggtt ggtggcgcac ttcctgaaac
     4801 gggcagtgta ttcaccatgc gtaaagcaat caga
//
LOCUS       DAOFSV010000236         4806 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.236, whole genome shotgun sequence.
ACCESSION   DAOFSV010000236 DAOFSV010000000
VERSION     DAOFSV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.236"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            65..394
                     /locus_tag="QBH27_004566"
                     /pseudo
     CDS             65..394
                     /locus_tag="QBH27_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(503..1435)
                     /gene="mmuM"
                     /locus_tag="QBH27_004567"
     CDS             complement(503..1435)
                     /gene="mmuM"
                     /locus_tag="QBH27_004567"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HDQ7016785.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(1422..2825)
                     /gene="mmuP"
                     /locus_tag="QBH27_004568"
     CDS             complement(1422..2825)
                     /gene="mmuP"
                     /locus_tag="QBH27_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HDQ7016786.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            3033..4049
                     /locus_tag="QBH27_004569"
     CDS             3033..4049
                     /locus_tag="QBH27_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HDQ7016787.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
     gene            4277..4594
                     /locus_tag="QBH27_004570"
     CDS             4277..4594
                     /locus_tag="QBH27_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HDQ7016788.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(4544..>4806)
                     /locus_tag="QBH27_004571"
     CDS             complement(4544..>4806)
                     /locus_tag="QBH27_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7016789.1"
                     /translation="YRIRQSMSRRGNCWDNSPMERFFRSLKNEWVPATGYVSFSDAAH
                     AITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAVASFS"
BASE COUNT         1248 a         1385 c         1218 g          955 t
ORIGIN      
        1 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
       61 taccgccaac ctgttcccgg caaccttcag cgacggatac gttgatatct acggctatca
      121 tctgccgcgc ccgctgcact ttggtacaca gggtgaaggg atggtcggtg tgcgcccgga
      181 agcgatcacg ctcagcgatc gcggcgaaga gagccagcgc tgcgtgatcc gccatgtcgc
      241 ctatatgggg ccgcagtatg aagtgacggt ggaatggcac gggcaggaga tattattgca
      301 ggtcaacgct acgcgtctgc aaccggacgt cggcgagcag tattatcttg aaatccatcc
      361 gtacggcatg tttgttctgg cggatgcggc ataacgtttt gtggttttgt aggccggata
      421 aggcgcttgt gccgccatcc ggcaatgcct gatgcgctgc gcttatcagg cctacaatga
      481 acgatgtaga cccgataaac cctcagcttc gcgcttttaa cgcggcgata tccgcaggcg
      541 tggtgcgaca gcacccgcca atcaaccgtg cgccagcggc ctgccactgc ggcagataat
      601 ccgccagctg cgcgcaatgt tcaccgtgat gatgccaggt tttgctcacg gcatcgtaat
      661 gctcgcccga gttcggatac accaccagcg gcagcaccgt taaaccgtgt aaatgctgca
      721 acgcagcggt ggtgttttcc agcgcaatac agttaatgcc tagcgccacc acctgcggat
      781 aacccgccag caacgcaacc acgtcacgca gcggcgtacc gtcgctcagg tgttcgctgt
      841 cgcgcagggt aaatgagaac cacgcccgcg cacgcggata tgcggtcaac agctcggcca
      901 acgcctcaat ctcggaaaaa ttcggcaggg tttcgcaggc cagcagatcg gccccggcat
      961 ccagcaaggc ttccacgcgc gggcgatgaa acgcctgaaa tgcctcaacg ctacaatgat
     1021 aatcgccacg gtattcagag ccatccgcca gatacgcgcc gtaaggcccg acggatcccg
     1081 ccaccagaag cgttcccgcc tgcgggttct ctgccagata cgcttcacgg gctttacgcg
     1141 ccagctccac gcttttgcca atcagcgctt tcgactgcgc ttcatccaga cctcgcgcgg
     1201 cgaagcccgc cggcgtcgcc tgatagctgg cagtgatcgc gcattgcgcc cccgcccggt
     1261 agtaatcaag atgcacttcg cggataagct ccgggttttc taccagcact ttggctgacc
     1321 acaggctgtc ggctaagtta caccctcgcg cttccagctc cgtcgccatc gcgccatcca
     1381 gcagcaggat atcctgttta tcaagaagag cgcgtaacgg attattctgc gacatgttct
     1441 ggctcctgtt ttgcgtttcg gggttgagta aggaaataag caccatagca caacgcaaca
     1501 aacggtaacc cgcaccacaa cgcaattctc tgcgctggat cgaatgccag cccaacacag
     1561 gccaccaggc acagcacaaa acctaatact ggcaccagcg gataccacgg cgcgcgataa
     1621 tgtaattcac tcaatgcctt accttgttgc agatgacggc gacgaaaaac aaaatgcgag
     1681 gcgcagatac tcagccacac cgctaccacc gcaaacccgg agattgccga cagcgcaaca
     1741 aataccgtgt ccggggccac cacgctggaa aacagcgcca gcacaccacc gagcatactg
     1801 accgacagcg ccgtcagtgg cacgccgttt ttcgttactc gcgcaaaaca ggccggtagc
     1861 gtacgttcat tcgacaacga ccacagcatg cgcccggagg catataaccc ggagttcgct
     1921 gcagaaagaa tagccgtcag gatcacgaag ttaaaaatat cagcggcgta cgggatccct
     1981 actttctcaa ataccagcac aaacgggctt ttctccacgc ccgcctgctg catcgggatc
     2041 agcgctgcca gcacaaacac ggtgccgata aagaaaataa tcagtcgcgc gatggtagta
     2101 cgaatcgcta ccgggataac tttgcgcggg ttttccgttt caccggcggc aatgccgata
     2161 agctcggtac ccgaaaaagc aaagttcact gccaccatag tcatcaaaat cggtaagcca
     2221 ccgtgcggga accagccttc tgccgtgata ttactcagcc ccggcgcggg cgagccatcc
     2281 tgcatcggaa taaagccgaa aatcgccgcc ccaccgagga tgataaaggc gatgatagtg
     2341 accactttga ccagcgagaa ccagaactcc ccttcggcaa aaaagcgcgt ggagataaca
     2401 ttcagaccaa aaataatcgc gcagaacacc acgcaccaga cccataccgg cacctgtgga
     2461 aaccagtact gcatacagaa tccagcggcg gtaaagctcg aacccagcgc cacggtccag
     2521 gtcagccagt aaagccaggc cacggtatac ccggtagccg gaccaagata gcgcgcggca
     2581 taaacgtgaa acgctccggt ctccggcatc gcgaccgaca gctcgcccag acactgcata
     2641 accagccaga ccaccagcgc accaatcaga taggccagca gcgttcccgc cgctccagtg
     2701 gtggaaatga tgtacccggt attgaagaat aatcctgtgc caatcacgcc gcccaaggaa
     2761 agcataatca ggtgacgcgt tttcattgtg cgcttcagtg gcgcattttg ttgtgttgtt
     2821 tgcatataac cttctaaacg tatggacatc taaacatcca aagaaggttt gttataccgc
     2881 gtttgttaac agaatgcaaa gtcaaccctg caccagacgc agatgcccgc gccgggcgct
     2941 taatagcggg tggggattaa taatcagata caaaaagggc cgccagctca taacgcctcc
     3001 ggaaggtgcg aacaagtccc tgatatgaga tcatgtttgt catctggagc catagaacag
     3061 ggttcatcat gagtcatcaa cttaccttcg ccgacagtga attcagcagt aagcgccgtc
     3121 agaccagaaa agagattttc ttgtcccgca tggagcagat tctgccatgg caaaacatgg
     3181 tggaagtcat cgagccgttt taccccaagg ctggtaatgg ccggcgacct tatccgctgg
     3241 aaaccatgct acgcattcac tgcatgcagc attggtacaa cctgagcgat ggcgcgatgg
     3301 aagatgctct gtacgaaatc gcctccatgc gtctgtttgc ccggttatcc ctggatagcg
     3361 ccttgccgga ccgcaccacc atcatgaatt tccgccacct gctggagcag catcaactgg
     3421 cccgccaatt gttcaagacc atcagtcgct ggctggccga agcaggcgtc atgatgactc
     3481 aaggcacctt ggtcgatgcc accatcattg aggcacccag ctcgaccaag aacaaagagc
     3541 agcaacgcga tccggagatg catcagacca agaaaggcaa tcagtggcac tttggcatga
     3601 aggcccacat tggtgtcgat gccaagagtg gcctgaccca cagcctggtc accaccgcgg
     3661 ccaacgagca tgacctcaat cagctgggta atctgctgca tggagaggag caatttgtct
     3721 cagccgatgc cggctaccaa ggggcgccac agcgcgagga gctggccgag gtggatgtgg
     3781 actggctgat cgccgagcgc cccggcaagg taagaacctt gaaacagcat ccacgcaaga
     3841 acaaaacggc catcaacatc gaatacatga aagccagcat ccgggccaag gtggagcacc
     3901 catttcgcat catcaagcga cagttcggct tcgtgaaagc cagatacaag gggttgctga
     3961 aaaacgataa ccaactggcg atgttattca cgctggccaa cctgtttcgg gcggaccaaa
     4021 tgatacgtca gtgggagaga tctcactaaa aactggggat aacgccttaa atggcgaaga
     4081 aacggtctaa ataggctgat tcaaggcatt tacgggagaa aaaatcggct caaacatgaa
     4141 gaaatgaaat gactgagtca gccgagaaga atttccccgc ttattcgcac cttccctaga
     4201 acaacaatgc ctcgattggt ttaaagagct aggttaccaa taccaaaatg gctatgacat
     4261 cgcaccagat ggtgatatgc ctgaacgtga aaactatcag caagtcacgc tacaggcgcg
     4321 tttagtctct gccttggaaa ccttaaaccc aaatgtgccc agtgacacct taatcgaagt
     4381 agcgaaaatc gttgccacac cgcaaacgcc tgtactgatt aaaaacaaca aactctttca
     4441 ccaatattta atcgaaggtg tgcctgttga atactcggta atggagcaag aacaatgggt
     4501 gaccaagcat actcatgttc gattaattga ttttgtgttg tggtcaacta aaactggcca
     4561 ccgcgttaga gtttttccag tatcggtttt ctgattcgtt tggtggtaac ccaccattat
     4621 attcgtgcgg tcttagtgcg ctgtaatatc caacgatata gtccgttatg gcgtgagctg
     4681 catcgctgaa acttacatag cccgtcgctg gcacccattc gttcttcaga ctcctgaaga
     4741 agcgctccat tgggctgtta tcccagcagt ttccacgccg actcatactc tgcctgatcc
     4801 ggtatc
//
LOCUS       DAOFSV010000237         4770 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.237, whole genome shotgun sequence.
ACCESSION   DAOFSV010000237 DAOFSV010000000
VERSION     DAOFSV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.237"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..171
                     /locus_tag="QBH27_004572"
     CDS             <1..171
                     /locus_tag="QBH27_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDQ7016790.1"
                     /translation="GKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDK
                     VIGHYLNIKHYQ"
     gene            complement(308..1240)
                     /locus_tag="QBH27_004573"
     CDS             complement(308..1240)
                     /locus_tag="QBH27_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016791.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
     gene            complement(1244..2239)
                     /locus_tag="QBH27_004574"
     CDS             complement(1244..2239)
                     /locus_tag="QBH27_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDQ7016792.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            2947..3165
                     /gene="ccdA"
                     /locus_tag="QBH27_004575"
     CDS             2947..3165
                     /gene="ccdA"
                     /locus_tag="QBH27_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDQ7016793.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            3167..3472
                     /gene="ccdB"
                     /locus_tag="QBH27_004576"
     CDS             3167..3472
                     /gene="ccdB"
                     /locus_tag="QBH27_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDQ7016794.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            3473..4279
                     /locus_tag="QBH27_004577"
     CDS             3473..4279
                     /locus_tag="QBH27_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDQ7016795.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
BASE COUNT         1220 a         1134 c         1202 g         1214 t
ORIGIN      
        1 ggaaagctgc acgtaatcag caagcgttac actcagcgaa ttgagcggca taacctgaat
       61 ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag
      121 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcagta agttggagtc
      181 attacctgtg ttactaaccg tctgaggcga aggtgactat gggtttacgc tgacactcag
      241 gcaaccaaga attgtttatc ggatgtgctc cggcatacgt aaatcctaca ccggagctaa
      301 ggagggatta cttctgaagg tgcagctcct tcagatttgt ttcaaagtaa tcaacataaa
      361 ttcgttcatt tttgggcttc attatcagct gaataccgtt gggtacgggc ggacatgagg
      421 cccggtaggt aaacatctca tcttggatat tccggagcgt ataagccagt tgctcttctt
      481 ctgcccttcc ggacaccctt atgccggata attccttctc aacgtacttt ttcagcttct
      541 cgcttctttt catactgctg cgattatcca gaaactgaag caagaaatag cggatgaccg
      601 gcagtgtgaa caccacatag aagaggatct cattcagccc ggatttcatg acgatgccgt
      661 aaacaagcat cgcgacaggc gagacaataa aagcggaaag ataaaatacg tgcagaacct
      721 ccctgagcga tttgtcccag ccgagatttt ttccgtggca aaggagcgcc attacggcct
      781 gcggcgcgtc atacacctta ctgacgtacc acccctcttc gccaaatcgc tttcgtgctt
      841 tttccgggct gtctctaaaa tatgcagctt cccggatgat gtcatcccgt gggatctcct
      901 ccaccttcag ttcattacgc cggatgccaa gtacccggca gtcgtacatt tcctggattg
      961 aggctgcctt cctgcgcaga gccgagatgt gtccactgat aacaatgtta aaggtaagca
     1021 ccaggagtcc atataacccc aacagcgaag acaacaactc tgaatcgaca aattcccggc
     1081 tccggttgta tacggtaacg aaagaaagca ggagcggtac gatgatgctg aagagaaaat
     1141 tcagtctgtt ccagtttttt accctgtcgt agacacgggc ctgcgcatac agtaaatcca
     1201 tgttttctct ggtcgactgg tttgtgtaaa tggcattcat gattcacccg taaacaggaa
     1261 agaatggccc gaacacatcg cgccatttat tgatggattt ttcatactct ttattatttt
     1321 caaaccatct cgcctccgat accttttgcg catcgagata acatctgtcc gatatcgcct
     1381 tacgtgcctc ggctgaaagt gagttgatat caccctgtat gcctttggga tcgttgacag
     1441 agtaccgcac ggattgacca aggtggcgga aaagcgcttc cagctccatg tcaacaaacg
     1501 atgagcagga tgtcctgcca gcgtaatagt ccagaatcag cgtttccagc aggtaggaac
     1561 tcatggatgg catcgtcggt cgccgctgcc agtatttgac tgctctgatg acattgagca
     1621 cattgccgtt attctgcaca ttgatagtag tgactctgtc cctgtcttta cgggggtcgg
     1681 taaacttcca gtggccgttg ccatcgggga tcaggtagta agtccgtcca aaggcatctt
     1741 cgctggtaat aaagcagggg acgatgtcaa aattccagtc cttgctgacc agtttcagcg
     1801 tgacagcttc ctggttacgc ctgatatcag cctgggcgta ctgggcaatg tcctgcaggc
     1861 ggtttttaaa ggcattcaga attctgacgg agcagacggt gtcagctccg gaatggcggt
     1921 aactgtgcag acgtgacccc tctccggagg aggttacagt tatccggtca ctcaggatgg
     1981 tataggtggc accttgcccg gttaagccaa acatcagatc gatatcatca agcggacgga
     2041 ttttggttct tctggcaaag gagccaaagg caatatggat cgctggaaaa ctcaccggaa
     2101 atttatcgtc cttctcaaaa tcattcatct taccgataag ccagtcgcga cttgcacgcg
     2161 cgtcatcggt atctgccttt ttgagattca cggtatcttt cataaattcg ttaaaggcag
     2221 ctattactgt ggtcgccatg ttcagtcctt taattattta ttgcaaaagg cgcggggctc
     2281 tgtcgtacat ctgaacattc cagtgcccgg gaagagtcgg tacagtttat tattagtgcc
     2341 gcaagttcac taccgtctcg ctctctgcaa gggcatataa tttacatggg aaatatgagc
     2401 ctcgttcttt cattgataat tatgcaaagt atttcagtta ttaatgccag ataagcaata
     2461 atgcaattaa gttcaaaata tatcattagt attgcatcat cagcacaaat ccttcttccc
     2521 ctctccagag atccgaaaaa agtaagttac ggatcttcct ctccctccgc acagcgttac
     2581 atcccgtcag cacagcatgt agtgcctcat acagttgcca atggcactat atgttgtgtt
     2641 gtatctctgg actgtgatgc gccgcgcagg ggcggaaaac agcgatatga tgattttctc
     2701 agcgttgtac acttccggaa agtcgtttat tcaaataaag tcggatccat acgaaacggg
     2761 aatgcggtaa ttacgctttg tttttataag tcagatttta atttttattg gttaacataa
     2821 cgaaaggtaa aatacataag gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     2881 tgatttttgc ggcataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     2941 tgtgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     3001 gcatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     3061 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     3121 attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt
     3181 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     3241 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     3301 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     3361 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     3421 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     3481 ctccgttata cacagccagt ctgcagtcat ggtaccggca gtgtattctg ccgggcagcc
     3541 tgcatcgctg cctgttgcca ttgattatcc ggcagctctg gcacttcgcc agatgtcgat
     3601 ggtccatgat gaactgccaa aatatctgct ggctccggaa gtgagtgccc tgctccatta
     3661 cgtcccggat ctgcaccgca agatgctgct ggccacactg tggaacaccg gagcacgcat
     3721 taatgaagca ctggcgctga cgcgggggga tttttcgctt gcgcctccgt atccgtttgt
     3781 gcagcttgcg accctgaaac aacggaccga aaaagctgcc aggacggcgg ggagaatgcc
     3841 cgccggtcag cagactcacc ggctggttcc gctctctgac tcctggtacg tcagccagct
     3901 gcagacgatg gtggcaacac tgaaaatacc catggagcgg cgtaaccgtc gcacaggaag
     3961 gacagagaaa gcgcggatct gggaagtgac ggacagaacg gtcaggacct ggattgggga
     4021 ggcggttgcc gccgctgctg ctgacggtgt gacgttctct gttccggtca caccacatac
     4081 gttccgccat tcctatgcga tgcacatgct gtatgccggt ataccgctga aagttctgca
     4141 aagcctgatg ggacataagt ccatcagttc aacggaagtc tacacgaagg tttttgcgct
     4201 ggatgtggct gcccggcacc gggtgcagtt tgcgatgccg gagtctgatg cggttgcgat
     4261 gctgaaacaa ttatcctgag aataaatgcc ttggccttta tatggaaatg tggaactgag
     4321 tggatatgct gtttttgtct gttaaacaga gaagctggct gttatccact gagaagcgaa
     4381 cgaaacagtc gggaaaatct cccattatcg tagagatccg cattattaat ctcaggagcc
     4441 tgtgtagcgt ttataggaag tagtgttctg tcatgatgcc tgcaagcggt aacgaaaacg
     4501 atttgaatat gccttcagga acaatagaaa tcttcgtgtg gtgttacgtt gaagtggagc
     4561 ggattatgtc agcaatggac agaacaacct aatgaacaca gaaccatgat gtggtctgtc
     4621 cttttacagc cagtagtgct cgccgcagtc gagcgacagg gcgaagccct cgagtgagcg
     4681 aggaagcacc agggaacagc acttatatat tctgcttaca cacgatgcct gaaaaaactt
     4741 cccttggggt tatccactta tccacgggga
//
LOCUS       DAOFSV010000238         4720 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.238, whole genome shotgun sequence.
ACCESSION   DAOFSV010000238 DAOFSV010000000
VERSION     DAOFSV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.238"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..915
                     /locus_tag="QBH27_004578"
     CDS             <1..915
                     /locus_tag="QBH27_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HDQ7016796.1"
                     /translation="DHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQL
                     NLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELN
                     SLSDKIQKLSDDAATANADPADMAAQVALITSRINDLTASVILMHAPKGVAVASGEHL
                     QLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIKLIANKGAVSVQAQHDL
                     MELLAKKSIEIVSTEDEIRISAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYG
                     RMGKAHEPVELQMLAEKVDEPPVKFFFS"
     gene            927..3290
                     /locus_tag="QBH27_004579"
     CDS             927..3290
                     /locus_tag="QBH27_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HDQ7016797.1"
                     /translation="MIISPPFIRAKNNNENDAHWIERMMPVEPDRDYPINYGGSWHGG
                     IHVRHTNSDRQPEYVRAIADGVVVSIRNPSDQAACSLPPLNYNGSTDDGYVLLRHETE
                     IGTGEDGNIVYYSLYMHLEKIEEGISEGNKVYRKSSLGMPGHVDGDRGLHFQIFCDDD
                     NLRKITGRTTPEMDVSRHGRTDVVYGDIYFYLPPGTRFYRSAPENNAVDNTGEVFQST
                     EPLVVTMCFDTGHCIMTTRRANPVIPSRYDIVGEVLTDADGTDYEYNLYSAALRLYPQ
                     SPSAGFELLRFGRVINTEHETLIPANAPLWRTVSYPGGQGVINLAADVVTKFSDGDFP
                     HWSGWRLVDDDADSNSQCNSVILRGGGFTDFRQMICHFPLEWCNTGIEARYGWLKEGV
                     IQERQENEANSDLSLGTDYYRPNNVEYNTTGDSHGESPQSAISEMPDLSYEQSNAYEN
                     AVRVLLLGEEDWQTLVKHIKALCFDTSSIAEGRVWHFNPGEFIKHFRKCGWVDKAILK
                     RIFASGTNDRDRAVIEQKVDIYGTAINIVMNKYIMNSPLRRAHFLGQGAVESSRLRSM
                     QEKSQYQTVDENGRPVGGGIVPDSLRDENSDLGHWYGAIETEVDGYFSGVKYNSGGGR
                     IAGSYDWILGNCDTEDAQKFRGRGFKQLTGRSNYAEYWVYRAWIDTNSFTAKWWEDPL
                     WRLHDRRRLTRIPANIEEPHRVTRSEYNCIDTGGFFIVKTVDRRGTRSSITRAMDQDS
                     EVIHLNNTTIDTTDEQTIRSVSRAINGGENGLPDRITFTRAAKKVLI"
     gene            3287..4144
                     /locus_tag="QBH27_004580"
     CDS             3287..4144
                     /locus_tag="QBH27_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016798.1"
                     /translation="MKKSFFLFLYFISLSAYPGDENAYIYGNDGTIEGIKYNYLDNVI
                     KFNIGDMSFDVFSKDNFFLKKCLLDNYSDSIDTGISFLTTDHEAIVFQSSGRFVLTKD
                     VIDICNSSEEKHAEVYPPVNIGMIADMNFRKNVALIYHPMGNISGKMVYQAELSKFVK
                     KNYRSSSFLTEVDPEKIQYLITGRFFYNGSYRDLEKKSYSGPFDLVSGVISPDAKYVD
                     PFGLGCDIDKVNHSIPHVYEISTAQRVAFKSDPALSAIINKEQIDKKCRQLLKASASL
                     KDLGGYLSQ"
     gene            4608..>4720
                     /locus_tag="QBH27_004581"
     CDS             4608..>4720
                     /locus_tag="QBH27_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family domain
                     protein"
                     /protein_id="HDQ7016799.1"
                     /translation="MDVPVLNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTP"
BASE COUNT         1412 a          868 c         1191 g         1249 t
ORIGIN      
        1 gaccatgtga ccattcagaa ctacaagcgt aacgtgctgc gcaccccggc gaacaacaaa
       61 atccgcctcg acgatgagcg cggcaaagag catatcaagg tcagtaccga gtacggcggc
      121 aagagccagc taaacctcgg ccatctggtg gatgccggaa aacagcagcg cggggaaggt
      181 tttgaactgc gtaccgatct gtggggtgca gtacgggcaa agaaaggcat ctttatcagc
      241 gccgacgcgc aggataaggc gcagggtcag gtacgcgaaa tggccgacat tatcagcgaa
      301 ctgaacagcc tgagcgataa aatccagaaa ctgtctgacg atgcggccac ggcgaacgcc
      361 gatccggcgg atatggccgc gcaggtggcg ctgataacca gccggataaa tgacctgacc
      421 gcctcggtga ttttgatgca cgcgccgaaa ggtgtggcgg tcgccagcgg cgagcacctg
      481 caactggccg cagtgaaaaa cctccagata aatgcgggaa acaacgccga tatcggcgtg
      541 gtgaagaata tgtttatcgg cgtagggcgg gcgctgagcg tgtttgtgcg caaggcgggt
      601 atcaaactga ttgccaataa aggcgcagta tcggtacagg cgcagcacga tctgatggag
      661 ttactggcga agaagtcgat agagatcgtc agtactgagg acgagatcag gatttcagcg
      721 aagaaaaaaa taacgattaa tggtggtgga agttatatcc ggattgaggg tagcggcatt
      781 gagccgggaa cgccggggga ttataacgtc aaagcggtgc attacgggcg tatgggaaaa
      841 gcacatgagc ctgttgagtt acaaatgttg gcagagaaag tagatgagcc acccgtaaaa
      901 tttttctttt cttaaggata taagaaatga ttattagccc gccatttata cgagcgaaaa
      961 ataacaatga aaacgatgct cattggattg aaaggatgat gccagttgaa cctgaccgtg
     1021 actatcccat caactatggg ggatcatggc atggaggcat ccacgttaga catacaaaca
     1081 gtgacaggca accagaatat gtccgtgcca ttgctgatgg tgtcgtggta tctattcgca
     1141 atccctctga ccaggcagca tgtagtcttc cgccgctcaa ttataatgga agcacagacg
     1201 atgggtatgt tctgcttcgt catgaaactg aaataggcac tggtgaggac ggcaatattg
     1261 tttattactc tctttacatg cacctggaaa agattgaaga gggtatcagt gaaggaaata
     1321 aagtctaccg gaaatcgtca ctgggaatgc cggggcatgt cgacggggat cgcggcctgc
     1381 acttccaaat tttctgcgat gatgacaatc tgagaaaaat aaccgggcgc actacacctg
     1441 agatggatgt gagccgtcat gggcgtaccg acgtggttta cggggatatc tatttttacc
     1501 tgccgcccgg tacccgcttt tacaggagtg cgccggaaaa caacgcagta gataacacag
     1561 gtgaagtttt tcagagcacg gaaccgttgg ttgtcaccat gtgttttgat actggccatt
     1621 gtatcatgac tacccgcaga gcaaatccgg tgataccaag ccgttatgac attgtgggag
     1681 aagtgttgac ggatgctgac ggcactgatt atgaatacaa cttgtacagt gctgcactga
     1741 gactttatcc gcaaagtcca agcgcagggt ttgagttact gcgctttggt cgggttatca
     1801 ataccgagca tgaaacactg atacccgcga atgcgccact gtggcgaacc gtgagctatc
     1861 ccggtgggca aggagtgata aatctggcgg cagatgttgt gacaaaattt agcgacggtg
     1921 attttccgca ctggagtggc tggcgactgg tagatgacga cgctgacagc aacagtcagt
     1981 gcaattcagt aatattacgt ggtggtggtt ttacggattt tcgtcagatg atttgccact
     2041 tcccactgga gtggtgtaat acgggaatag aagcgcgtta cggctggttg aaagaagggg
     2101 taatacagga acgtcaggaa aatgaggcta acagcgattt atcattaggt acggattact
     2161 acaggccaaa taatgtcgaa tataatacga ctggagatag ccatggagaa tctcctcagt
     2221 cagctatatc agaaatgcca gatttgtctt atgaacagtc aaatgcttat gaaaatgccg
     2281 tcagagtttt attactggga gaagaagact ggcagacgtt ggtgaagcat attaaggcac
     2341 tgtgttttga taccagtagt attgctgaag gacgggtttg gcattttaat ccgggtgagt
     2401 ttattaagca ttttaggaag tgtgggtggg ttgacaaagc aatattgaag cgaatttttg
     2461 cctctggtac taatgaccgt gatagagcgg ttattgaaca aaaagttgat atatatggta
     2521 cggctataaa tattgttatg aataaatata taatgaattc tcctctgaga cgtgctcact
     2581 ttctggggca aggggcagtg gaatcttccc gtttaagaag tatgcaggaa aaaagccagt
     2641 atcaaactgt tgatgaaaat ggtcggccag tcggcggagg tattgttccg gactcattac
     2701 gtgatgagaa ttcggacttg ggtcattggt atggtgcaat tgagactgag gtcgatggat
     2761 acttctctgg tgttaaatat aattctggtg gaggacgaat tgcaggctca tatgactgga
     2821 tactaggaaa ctgcgacact gaggatgccc agaagtttcg tgggcgaggt tttaagcagt
     2881 taactggtcg atcaaattat gccgaatatt gggtctatcg tgcctggata gatacgaata
     2941 gttttaccgc taagtggtgg gaagaccctc tgtggcgctt acatgatcgt cgtcgtttaa
     3001 ctcgcatacc ggctaatatc gaagagcctc acagggttac cagaagcgaa tataactgta
     3061 tcgatactgg tggattcttt attgttaaga ccgttgatcg tcggggtact cggtcaagta
     3121 ttaccagagc gatggatcaa gacagtgaag ttattcattt gaacaatacc acgatagata
     3181 caacggatga acaaacaata agaagtgtgt cccgtgcaat taatggtggt gaaaatggtt
     3241 tgccagacag gataaccttc acacgcgcag ctaagaaggt tttaatatga aaaagagttt
     3301 ctttttattt ttatatttca tatccctgag tgcatatcct ggagatgaaa atgcatacat
     3361 atatggtaac gatggaacaa ttgaaggaat taaatacaat tatctcgata atgtaataaa
     3421 gttcaatatt ggtgatatgt cttttgatgt tttcagcaaa gataattttt tcttaaagaa
     3481 atgtcttctg gataactaca gtgacagtat agatacaggc atttcatttt taacaacaga
     3541 tcatgaagca attgtttttc agtcatcggg tcggtttgtt ttaacaaaag atgtaatcga
     3601 tatatgtaat tcttctgaag aaaagcatgc ggaagtttat ccacctgtca atattgggat
     3661 gattgctgac atgaacttca ggaaaaatgt tgctcttata tatcacccaa tgggtaatat
     3721 ttcaggaaaa atggtatatc aggctgagtt gtcaaagttt gttaaaaaaa attatagatc
     3781 aagcagcttc ttaacggagg ttgatcctga aaaaatacag tacctgataa cgggtaggtt
     3841 tttttacaat ggtagttaca gagatttaga aaaaaaaagt tattcaggcc catttgatct
     3901 cgtctcaggc gttatctctc ctgatgcaaa atatgtagat cctttcggtc tggggtgtga
     3961 tattgataag gtcaatcata gtataccaca cgtgtatgaa atatccacag cacaacgagt
     4021 tgcatttaaa agcgatccag cgttatctgc gataataaat aaagaacaaa tagataaaaa
     4081 gtgtcggcag ttattaaaag catctgcatc tttgaaagat ttaggtggat atttaagtca
     4141 gtagaatatg tcaccgttac tctactgtgc tataggagat agcgaagaag agttttttca
     4201 atcaaatatt gccagagttt tattattggt tgaagaagac ttgaagacgt tggtgaacaa
     4261 tttcaagagg ctatttttta atgccaatgg tattgctgca ggataagttt ggcattttat
     4321 ctcaggtaac tttataaatt atttccataa atgcggaaaa atcacaaaaa gcttttttat
     4381 ttcttcattc ttttatgata ggtcgtcagg aacaacactg accagcatat ttaggaggag
     4441 agcgtatata tttttcacga agtttcatca aactatacga cagtacagaa gaattgtcat
     4501 attttaaatt ataactctaa tgtaataatt cattatccca gaatgtatcc gaacaagaaa
     4561 taacggctcg ttattctttg ttagataaat gaaggagtac aaagaaaatg gatgttcctg
     4621 ttctgaattt cgaccactca caccacaagc tcaaaattcg cggcctgcaa agtccggtgg
     4681 atgtgctgac cttcgaaggc cgcgaacaac tgagtacccc
//
LOCUS       DAOFSV010000239         4713 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.239, whole genome shotgun sequence.
ACCESSION   DAOFSV010000239 DAOFSV010000000
VERSION     DAOFSV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.239"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            9..578
                     /locus_tag="QBH27_004582"
     CDS             9..578
                     /locus_tag="QBH27_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HDQ7016800.1"
                     /translation="MTSRINDLTASVILMHAPKGVAVASGEHLQLAAVKNLQINAGNN
                     ADIGVVKNMFIGVGRALSVFVRKAGIRLIANKGAVSVQAQHDLMELLAKKSIEIVSTE
                     DEIKITAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYGRQPKASEKVPMPEFP
                     ILSAVDSSDFCLECLLNAIKNDDAVVEGV"
     gene            581..1402
                     /locus_tag="QBH27_004583"
     CDS             581..1402
                     /locus_tag="QBH27_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HDQ7016801.1"
                     /translation="MSSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYTPRVLKQLAARPDMDWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTVANNSDETVEIITLDEPLWQKIADRGDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQVVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            1446..1973
                     /locus_tag="QBH27_004584"
     CDS             1446..1973
                     /locus_tag="QBH27_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HDQ7016802.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAIPVAYFLLCVGIS
                     IWGAPTGGVILVIHSELDRPILGFSVNGMAGANAFAHGGGSTTCCGDIRGKEAEVVWT
                     LSTTRAQYNTGLREEVRRITLPLPERKHGQDFLHVHFLPGDKVFLGWSEGAGSPYEKR
                     KEPSYPSRKNQEAQP"
     gene            1970..3838
                     /locus_tag="QBH27_004585"
     CDS             1970..3838
                     /locus_tag="QBH27_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HDQ7016803.1"
                     /translation="MISESVISAAVRAQQSEDGKVGNCGRIWHVAFFFDGVGRNIDRD
                     ATDCRLSNIARLFRAYPDEQKNTGDTCYNKFYFSGMGTAYHDDSVDNIHALMDIGLDN
                     FLEDMKSLPEDTISDAGESVIKGEKKWTDVLGNLLEDLNNPVEWVKGIGKMAAKSVGK
                     AGIESTPWLRDSEAMSAYFMTGEPVRLEAAKRLFKKFYTENMKGDVPIKKISVSLYGF
                     DLGATLARKFLDNFLKEVCLKNKEKKHQYKNALVDIAFIGLFDCSRHSPTSSNNGLDY
                     FFMWLGLPGKMIGSVLGEKAIDQDSPLPDVVNNALHLVAAHERRPWRGLYLLGKKTDT
                     ANKKYREELLPGCSEDIGGGLKPDEQKPSAELCRVALYNMYHAACQAGVPFPDFDTLD
                     QYDTKVASYFIMNDAVQGGSVNYWVSRYQREEVKDKVFSEAMQEKYLDNYFLWLGEQY
                     YMYQYERERLDKELTSAHRGKISDYGPLAGTGINPNTNADDIKSQISELDSLWGWLND
                     VKSVAIGLRNDFIVNPRKNDSRIKLKPQVYNAAVNRAVKFLEFSRAVYNDKNLPSSSS
                     LTANTLYSFFVHDIQKVDHTPSISEDFFLRRSSEIPDADEPPGNKDDGKKHTRRDD"
     gene            3944..>4713
                     /gene="vgrG"
                     /locus_tag="QBH27_004586"
     CDS             3944..>4713
                     /gene="vgrG"
                     /locus_tag="QBH27_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ7016804.1"
                     /translation="MDIPTLNFDHSHHKLKIRGLKSPADVLTFTGHEQLSSPFRYDIE
                     FTSTNKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARY
                     EVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQV
                     MQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNSAYSVVEKSVSTRDYNYREATAEMTTG"
BASE COUNT         1324 a          947 c         1244 g         1198 t
ORIGIN      
        1 tagcattgat aaccagccgg ataaatgacc tgaccgcctc ggtgattttg atgcacgcac
       61 cgaaaggcgt ggcggtcgcc agcggcgagc acctgcaact ggccgcggtg aaaaacctcc
      121 agataaatgc gggaaacaac gccgatatcg gcgtagtgaa gaatatgttt atcggcgtgg
      181 gacgggcgct gagcgtgttt gtacgcaagg cgggtatcag gctgattgcc aataaaggcg
      241 cagtatcggt acaggcgcag cacgatctga tggagttact ggcgaaaaaa tcgatagaga
      301 tcgtcagtac cgaagatgag attaaaatca ccgcgaagaa aaaaataacg attaatggtg
      361 gtgggagtta tatccggata gagggtagcg gcattgagcc gggaacgccg ggggattata
      421 acgtcaaagc ggtacattac gggcgtcagc ctaaagcctc ggagaaggtt cctatgccag
      481 aatttcccat cctttcagcc gtggactcat ccgatttttg tcttgaatgc ctgctgaatg
      541 ccatcaaaaa cgatgacgct gttgttgaag gagtgtaatc atgtcttcag aacaacaact
      601 ggcagaccac tggcttaagc gaaacgaaac gttaaacgcc tttattttat ttgaaggtgg
      661 agcgctctct caggaaatgc ttcagatcat gatggagcat tataaatacc gacgaatttc
      721 cctgtatcaa ctgcccgatc ttaaaaaggt agcagcttac ggtccctggt tggttcgtat
      781 tgaaagtgaa aaggatctgg cgatggtgct tgtcgggaaa ctacctgtcg caggcattac
      841 tttctccgaa ttgcagctgg agccactggc gtctcggctt gctgtgggat gtatggcaaa
      901 aacgccggat ggtcgtgaag ttctgctgag gtactacacg ccccgagtgc tgaaacaact
      961 ggctgcccgg cctgatatgg actggcatcc ggtaatgttt tctcagatag aatcatggtg
     1021 gatgctggaa gatacgcggt ggcaaaggct gaacatgccg cttactgtgg caaataacag
     1081 cgatgagaca gtggaaatca taacgcttga tgaaccgctc tggcagaaaa tagcggatcg
     1141 gggagatgta tctgcgcttt taagagagtg gcgcaccatg aaaacgagtg agcacttccc
     1201 gccctgtatt cagcgaaata tggtggagaa ggccttgaat aaggctcagg tggtcggatt
     1261 gacaaagcca ctggatcaga aaatttatgc gttgagctat ctgaatggaa agaaagcgta
     1321 tcttgagtca gccgaatttc aggtcatgct ggaaaaggtg aaacgaaatg aaatatcgct
     1381 gtctgacctt ggtcgccagt gacatacatc tttgccaacc aattgataaa agggaataaa
     1441 atctgatggg gctatttacc cgactggaaa aatttgacca aaagcttacg cgtggttatg
     1501 cccgctgggg gcgttgggtg tggcggatgc taatagcgat ccccgtggct tattttttac
     1561 tgtgtgtagg aatatcaatc tggggagctc caactggagg agtcatattg gtgatccata
     1621 gtgaactgga taggccaata ttaggattca gcgtgaacgg catggctggg gcaaatgcgt
     1681 ttgcacatgg tggaggttca acgacttgct gcggtgacat cagagggaaa gaagctgagg
     1741 tcgtctggac attgagtacg actcgcgctc agtataatac gggactgcgt gaagaagttc
     1801 gacgtatcac tctgcctttg cctgaaagga aacatgggca ggattttttg catgtacatt
     1861 tcttgcctgg tgataaggtt tttttgggtt ggagtgaagg agctggttcg ccctatgaga
     1921 aacgaaaaga accttcttat ccgagtcgaa aaaatcagga ggcgcagccg tgataagtga
     1981 atcagtgatt tccgcagctg tacgagcaca acagtcagaa gatggtaaag tgggaaattg
     2041 tggccggatc tggcatgtcg ctttcttttt tgatggagtg ggacgaaata tcgaccggga
     2101 tgcgacagac tgccgtttga gtaatattgc ccgcctgttc cgggcatatc ctgatgaaca
     2161 aaaaaataca ggtgatactt gttataataa attttatttc tccggaatgg gcactgccta
     2221 ccatgacgat tctgtcgaca atattcacgc tcttatggat attggtttag ataactttct
     2281 ggaggatatg aaaagtctgc cagaggatac tatatcggat gcaggggagt ctgttattaa
     2341 aggagaaaag aaatggacgg atgtgcttgg taatctcctt gaggatctca ataatccggt
     2401 agagtgggtt aaagggattg gcaagatggc ggctaagtcg gtaggcaaag caggaattga
     2461 gtccacacct tggttgcgtg atagcgaagc catgagcgcc tattttatga cgggagaacc
     2521 cgtacgcctt gaagctgcaa agcgattgtt taagaagttt tatacggaaa atatgaaggg
     2581 agatgttccc atcaaaaaaa tttctgtttc attatacggt tttgaccttg gagctacact
     2641 ggcacgtaaa tttttggata attttcttaa ggaagtttgc ctgaaaaata aagaaaaaaa
     2701 acaccaatat aaaaacgcgt tggtagatat tgcttttatt ggtctttttg actgttctcg
     2761 gcattcaccg actagtagta ataatgggtt ggactatttt tttatgtggt tgggtttacc
     2821 agggaaaatg attggttcag tattaggtga aaaagctatt gatcaggact caccactgcc
     2881 ggacgtagta aataacgcac ttcaccttgt agcagctcat gaacggcgtc catggcgtgg
     2941 tctgtatctg ttggggaaaa aaacggatac agcgaataaa aaatacaggg aagaactgct
     3001 tcccggttgt agtgaagata ttggtggtgg cttgaaaccg gatgaacaga aacccagtgc
     3061 agaactgtgc agggttgcac tatacaatat gtatcatgca gcatgtcaag ctggtgtgcc
     3121 atttcctgat tttgacactt tggatcaata tgatacgaaa gttgccagct attttattat
     3181 gaatgatgct gttcagggag gatctgtaaa ttactgggtt agtcgttatc aaagagagga
     3241 agtgaaggat aaagtattta gtgaggcaat gcaggagaag tatctagata actattttct
     3301 ctggttgggt gagcaatatt atatgtatca gtatgaacgg gaacgtctgg ataaggagtt
     3361 gacatcggct catcgtggga aaataagtga ctatgggcca cttgcaggta cgggaattaa
     3421 tccaaatact aacgctgatg atataaaatc gcagatttca gaacttgact cactatgggg
     3481 atggttgaat gatgtaaaaa gtgtagctat tggattacga aatgacttta tagtcaaccc
     3541 gaggaaaaat gatagccgaa taaaacttaa acctcaggtt tataatgctg ctgtcaatcg
     3601 ggctgtgaaa ttccttgagt tttctcgcgc agtttataat gataaaaatc tgccatcatc
     3661 atcatctttg acggcaaata cactatattc cttttttgtg catgatatac aaaaggttga
     3721 tcacacacct tctatatctg aagatttctt cttacgtcgc tcatctgaaa ttccagatgc
     3781 ggatgaacct ccaggaaata aagatgatgg aaagaaacat actcgcaggg atgattagaa
     3841 attataagta gcttacttta attagttata ttttttgcat ttttcccttt ttaaagagaa
     3901 tcatacttgg atttattttt cataaatata aaggagtaca gagatggata tcccgactct
     3961 gaatttcgac cactcacacc acaagctcaa aattcgcggc ctgaaaagcc cggcggatgt
     4021 gctgacgttt acgggccacg aacagctcag ctcacccttc cgctacgaca ttgagtttac
     4081 cagtaccaat aaagcaataa cgccggagtc ggtgctgatg caggacggcg cattcagcct
     4141 gacggcaccg cccgtgcagg gaatgccggt gcagactgcc ctgcgcaccc tgcacggcgt
     4201 cattaccagc tttaaacacc tctcatcgtc acaggacgaa gcccgttacg aagtgcgcct
     4261 tgaaccccgt atggcgctgc ttacccgcag ccgccagaac gccatctacc agaaccagac
     4321 ggtgccgcaa atcgtcgaaa aaatcctgcg cgagcgccat caaatgcgcg gtcaggactt
     4381 cgtgtttaac ctcaaaaacg aatacccggc ccgtgaacag gtgatgcagt acggtgagga
     4441 cgacctgacg tttgtctcac gcctgctgtc ggaggtgggt atctggttcc gctttgccac
     4501 cgacgcccgg ctgaaaatcg aagttgtcga gttttatgac gaccagagcg gctatgagcg
     4561 cggactgacg ctgccgcttc gtcatccttc agggctgttt gatggtgaaa cggaagccgt
     4621 atgggggttg aacagcgcgt acagcgtggt ggaaaaaagc gtctccacgc gggactacaa
     4681 ctaccgtgaa gccacggcgg aaatgaccac agg
//
LOCUS       DAOFSV010000240         4683 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.240, whole genome shotgun sequence.
ACCESSION   DAOFSV010000240 DAOFSV010000000
VERSION     DAOFSV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.240"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..803)
                     /gene="chbR"
                     /locus_tag="QBH27_004587"
     CDS             complement(<1..803)
                     /gene="chbR"
                     /locus_tag="QBH27_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDQ7016805.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP"
     gene            complement(811..1161)
                     /gene="chbA"
                     /locus_tag="QBH27_004588"
     CDS             complement(811..1161)
                     /gene="chbA"
                     /locus_tag="QBH27_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDQ7016806.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1212..2570)
                     /gene="chbC"
                     /locus_tag="QBH27_004589"
     CDS             complement(1212..2570)
                     /gene="chbC"
                     /locus_tag="QBH27_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDQ7016807.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(2655..2975)
                     /gene="chbB"
                     /locus_tag="QBH27_004590"
     CDS             complement(2655..2975)
                     /gene="chbB"
                     /locus_tag="QBH27_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDQ7016808.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3274..3612)
                     /gene="osmE"
                     /locus_tag="QBH27_004591"
     CDS             complement(3274..3612)
                     /gene="osmE"
                     /locus_tag="QBH27_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDQ7016809.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            3814..4641
                     /gene="nadE"
                     /locus_tag="QBH27_004592"
     CDS             3814..4641
                     /gene="nadE"
                     /locus_tag="QBH27_004592"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDQ7016810.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAIRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
BASE COUNT         1303 a         1152 c         1022 g         1206 t
ORIGIN      
        1 ggcgtaaagg acgttaattt cttaaacgtt ttaataaaca agctcgggct actataaccg
       61 gcctcaaacg caatatccgt cactgaatag ttggtcattt ccagttgttt tttggcaaaa
      121 ttaatacgga tttcattaat aatctgcatt ggcgttttac cataatatcg ttgagtcgct
      181 cgcgtcaaat attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca
      241 ctaaactgct ctttatcatg cattttttct accgtacttt tcagccactg cggtatatca
      301 tcaatcacct gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc
      361 tcaacaaact cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca
      421 taggtgagaa aagcgttatt ggtccggtat acctgcgaag ctacaaagca ataaggcaac
      481 aacggcaggt aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg
      541 gcaccaaact cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc
      601 cgttccagta acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga
      661 gtaaattcat aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt attatagata
      721 aacacatgga agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc
      781 gcgttaatca ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat
      841 taattcagta atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac
      901 cagcaccaga ctcactttca tcttaccttc gccagcatcg ccttcaatca gtttcgtctg
      961 taccagatgc gcttcattca atgccatccg tgactgatcc atcatcgctt ttgctgcggc
     1021 aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc aggctacgcg cttgtccgga
     1081 gttgatgata agccccatca ccacttcttc cagctcttca gcttccgttt gcgtatcggg
     1141 aatattatcg agatccatca tacatcgttc ctctctattc ttaccggcac gattacccgt
     1201 accggcatcg attaaaattt cagggcgtta gcgatatctt cttcgctctc ttctttatca
     1261 atcgcgtttt gtgctttgtt agcgaccaca acaaagggca gataaattaa cgttgcgata
     1321 ccaaggttga agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct
     1381 cccagaccgg ttggcatggt ccacggtgca atattggtca ccggtggaat aatgcccatg
     1441 tagtacgctg ccagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac
     1501 atcaccgggt tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg
     1561 ccagacggca gcgccagttt tgccacctga cgataatcag cacgtcgaga ggcgataaag
     1621 atagccagga tcaggcctaa agtcgcacca ctgccaccaa ggaaaataaa ggagtccagc
     1681 atcggcttgg cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca
     1741 tattgctgat aggtcgcgat attttccagc gcccaaggcg tcataatgcc gttgtccagt
     1801 gcggtcagcg ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca
     1861 taggcccagc ctaccacgct acccaacgat gccagtgggg ttgagatcgt atccataatg
     1921 atctgatgga agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa
     1981 agaataataa aaccaggaat taatgctgag aaagagcgcg aaaccgatgt cggaacgcta
     2041 tcaggtaact taatgaccca gttacgatga acaataaagg taaacatttc tgccaccacc
     2101 aggccaataa taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca
     2161 taggcctcac cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaacccg
     2221 gccgccagcg catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc
     2281 attaaagaca ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca
     2341 ttaagtgttt caatggttga ggcatccagg cgaataccta aggaataaaa aaacgacccc
     2401 tccccaaagc ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac
     2461 ggcattaagc gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta
     2521 actgcaaaag ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac
     2581 ccttaaaaac cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga
     2641 aaatttaaaa taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc
     2701 acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg
     2761 ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca
     2821 tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga
     2881 acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc
     2941 gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc
     3001 tgtcagccag acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac
     3061 caacatgact ttttttgtgg ctgaaaggag tatacggtat aggcctgagc aatagtattt
     3121 ctttgacctg ataaattgtc tctccttgac ctgtggttat gaccctcttc acatttcggg
     3181 caaataattc gcgaagataa ttaagaacac aggccaaaaa aaaccggcgc aatggccggt
     3241 ttccgttgtt actcaagctt tcaggcgaag tgattacttc gtagcctgcg gatcagtgtc
     3301 gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac
     3361 aaagtaggtt tctgctttac catcacgttg acccaggatg taggtctggc aagtaccacg
     3421 agcatggatc atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg
     3481 gctcatgcct tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc
     3541 ataagccgta caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat
     3601 attcttgttc atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt
     3661 gggcgctttt tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgacg
     3721 ttttaaccac cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa
     3781 ggaagttttg tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag
     3841 gcgctgggcg caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tatcgatttt
     3901 ctgaaaagct acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc
     3961 ggtcaggact ccacgcttgc cggtaagctg tgccagatgg cgattaatga gctgcgccag
     4021 gaaaccggca acgaatcact gcaatttatt gccatacgcc tgccctatgg tgtccaggct
     4081 gacgaacaag attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt
     4141 aatatcaagg gcgcggtact cgccagcgaa caggcgctgc gcgaagcggg cattgaactg
     4201 agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt
     4261 gcgggtatga ccagtggtgt cgtggtgggc accgatcatg cagcagaagc tattaccgga
     4321 ttcttcacta aatatggtga cggcggtacg gacattaatc cgctgtatcg tctcaacaaa
     4381 cgtcagggta aacagttact ggcggcatta ggttgcccgg aacaccttta taagaaagcg
     4441 ccaacggccg atcttgaaga tgatcgccct tctctaccag atgaagtggc actcggcgtg
     4501 acctatgaca atatcgacga ctatctggaa gggaaaaacg tacctgaaca ggtcgccaga
     4561 acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc
     4621 gatgatttct ggaaaaagta ataatttgca cctattggat tgtgcgaaaa agagtaattt
     4681 gtt
//
LOCUS       DAOFSV010000241         4556 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.241, whole genome shotgun sequence.
ACCESSION   DAOFSV010000241 DAOFSV010000000
VERSION     DAOFSV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.241"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(49..1200)
                     /gene="argE"
                     /locus_tag="QBH27_004593"
     CDS             complement(49..1200)
                     /gene="argE"
                     /locus_tag="QBH27_004593"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDQ7016811.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQSVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIMGQSGHSSDPSRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1354..2358
                     /gene="argC"
                     /locus_tag="QBH27_004594"
     CDS             1354..2358
                     /gene="argC"
                     /locus_tag="QBH27_004594"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDQ7016812.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDIGEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            2366..3142
                     /gene="argB"
                     /locus_tag="QBH27_004595"
     CDS             2366..3142
                     /gene="argB"
                     /locus_tag="QBH27_004595"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDQ7016813.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSIKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3203..>4556
                     /gene="argH"
                     /locus_tag="QBH27_004596"
     CDS             3203..>4556
                     /gene="argH"
                     /locus_tag="QBH27_004596"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDQ7016814.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYSNATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSLVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     F"
BASE COUNT         1064 a         1122 c         1320 g         1050 t
ORIGIN      
        1 tcggataaga tgcgcaagca tcgcatccga cattgtttgg cctacgtttt aatgccagca
       61 aaaatggtga atcacctggg tgatcagttc gcgggtgggc ttgataaacc tcgtctccag
      121 atattcatca ggctgatgag cctgattaat tgagccaggc cccagaacca gcgtcgggca
      181 taacgtttga ataaacggcg cttcggtaca gtaattcacc acttcggtct ttgctccgag
      241 caatttctca accacttcaa ccagtttatg attcggtggg cattcatagc cagggatcgg
      301 cgggtgcagc tcgtcgaccg tcagacgacc cggccagcgt tcgctcactg gagccaatgc
      361 atcgttgagc aaaccattaa gttcattgag tgtcatgcct ggcagcggac gaatatccat
      421 atgcaactca caacaagcgc aaatacggtt agaggcgtcg ccgccgtgaa tatgcccgag
      481 gttgagtgtg gggtatggca cggtaaacgc ttcatagtga tagcgttctt tcaggttatc
      541 gcgcaattgc aaaatatggc cgatggcgtc gtgcatcagt tcgatagcgt taaccccgcg
      601 cgacggatcg ctggagtggc ccgactgacc cataattcga atggcatttg agatgtggcc
      661 tttatgtgcg cgtaccggtt gcagcgacgt tggctcgcca atgatggcgc aatccgggcg
      721 cagggcggta gtttcggcaa aataacgcgc tccggccata ctggtttctt catcggcagt
      781 cgccagaatg tagagcggtt ttgcaagctt cgtgacgtcg acatcgcgta gcgcatcaag
      841 gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag ccgtaaagct tgccgtcatg
      901 ctccgtcaac gtaaatggat cgcgcgtcca gcgtccgtca tcaaatggca ccgtatcggt
      961 atgccccgcc agcaacaaac cgccagcccc ctgtccgcaa ctggccagca tattaaattt
     1021 gttgcgagtt cctggcacag actgcacttc cacattgaag cctaaatcct taaaccagtc
     1081 cgccagcaga gtgattaaat ctgcattgct ttgatcgagt gcctcttccg tggcgcttat
     1141 tgaaggtgtg gcaatcagag cacggtaaat ctcgataaat ggcggtaatt tgtttttcat
     1201 tgttgacaca cctctggtca tgatagtatc aatattcatg cagtatttat gaataaaaat
     1261 acactaacgt tgagcgtaat aaaacccacc agccgtaagg tgaatgtttt acgtttaacc
     1321 tggcaaccag acataagaag gtgaatagcc ccgatgttga atacgctgat tgtgggtgcc
     1381 agtggctacg ctggcgcaga gctagtgacc tatgtaaatc gccatccgca tatgaacata
     1441 accgctttga ctgtttcagc gcaaagcaat gatgcgggaa agttaatctc cgatttgcat
     1501 ccgcagctaa aaggcatcgt tgatctgccg ttgcagccga tgtcggatat cggcgagttt
     1561 agcccagggg tggacgtggt gtttctcgcc accgcccatg aagtcagcca cgatttagcg
     1621 ccgcaatttc ttgaagcagg ctgtgtggtg ttcgaccttt ccggcgcgtt tcgtgttaac
     1681 gatgtcgcct tctatgaaaa atattacggc tttacccatc aatacccgga actgctggaa
     1741 caggcagcct acggtctggc ggagtggtgc ggtaataaat taaaagaagc gaacttgatt
     1801 gccgtgccag gctgttatcc gacggcagca cagctggcgc tgaaaccgtt gattgatgcc
     1861 gatcttcttg acctcaatca gtggccggtg atcaacgcca ccagcggcgt gagcggtgca
     1921 ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta gcctgcaacc gtatggcgtc
     1981 tttacccatc gccatcaacc agagatcgcc acacacctcg gtgctgacgt tatcttcacc
     2041 ccgcatctgg gcaatttccc gcgcggcatt ctcgaaacca ttacctgccg cctgaaaccg
     2101 ggtgtgagcc aggtgcaagt ggcgcaagcg ttacagcagg cgtatgccca taaaccactg
     2161 gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg tcgttggact gccattctgc
     2221 gatatcggtt ttgccgttca gggcgagcat ctgattattg tggcgaccga agacaactta
     2281 ctgaaaggcg cggcggcaca agcggtgcag tgcgccaata ttcgtttcgg ctatgcggaa
     2341 acgcagtctc ttatttaagg gtgcaatgat gaatccatta attatcaaac tgggtggcgt
     2401 actgctggat agtgaagagg cgctggaacg tctgtttagc gcactggtga attatcgcga
     2461 gtcacatcag cgtccgctgg tgattgtgca cggcggcggt tgtgtggtgg atgagctgat
     2521 gaaagggctg aatctgccgg tgaaaaagaa aaatggcctg cgggtgacgc ctgcggatca
     2581 gatagacatt atcaccggag cactggcggg aacggcaaat aaaaccctgc tggcatgggc
     2641 gaagaaacat cagattgcgg ccgtaggttt gtttctcggt gacggcgaca gcatcaaagt
     2701 gacccagctt gatgaagagt taggccatgt tggactggcg cagccaggtt cgcctaagct
     2761 tatcaacagt ttgctggaga acggttatct gccggtggtc agctccattg gcgtaacaga
     2821 cgaagggcaa ctgatgaacg tcaatgccga ccaggcggcg acggcgctgg cggcaacgct
     2881 gggcgcggat ctgattttgc tctctgacgt cagcggcatt ctcgacggca aagggcaacg
     2941 tattgccgaa atgaccgccg cgaaagcaga acaactgatt gagcagggca ttattactga
     3001 cggcatgatt gtgaaagtga acgcggcgct ggatgctgcg cgcacgttgg gccgtccggt
     3061 agatatcgcc tcctggcgtc atgcggaaca gcttccggca ctgtttaacg gtatgccgat
     3121 ggggacgcgg attttagctt aagttttgtt ggccggaggc gcagctttcc ggcattgaat
     3181 ttcaaaataa ggaaacagag ttatggcact ttggggcggg cgttttaccc aggcagcaga
     3241 tcaacggttc aaacaattca acgactcact gcgctttgat taccgtctgg cggagcagga
     3301 tattgttggc tctgtggcct ggtccaaagc cctggtaacg gtcggcgtgt taaccgcaga
     3361 agagcaggcg caactggaag aggcgctgaa cgtgctgctg gaagatgttc gcgccaggcc
     3421 acaacaaatc cttgaaagcg acgccgaaga tatccatagc tgggtggaag gcaaactgat
     3481 cgacaaagtg ggtcagttag gcaaaaaatt gcataccggg cgtagccgta atgatcaggt
     3541 agcaactgac ctgaaactgt ggtgcaaaga taccgttagc gagttgctga cggctaaccg
     3601 gcagttacaa tccgctctgg tcgagaccgc gcagaacaat caggacgcgg tgatgccggg
     3661 ttacacccac ctgcaacgcg cccagccggt gacgttcgcg cactggtgcc tggcctacgt
     3721 tgagatgctg gcgcgtgatg aaagccgttt gcaggatgcg cttaagcgtc tggatgtcag
     3781 cccgctaggc tgtggcgcac tggcaggaac ggcctatgaa atcgaccgtg aacagttagc
     3841 aggttggctg ggctttgctt cggcgacccg taacagtctc gacagcgttt ctgaccgtga
     3901 ccatgtgttg gaactgcttt ctgctgccgc tatcggcatg gtgcatctgt cgcgttttgc
     3961 tgaagatctg attttcttta acaccggcga agcggggttt gtggagcttt ctgaccgtgt
     4021 gacttccggt tcatcattaa tgccgcagaa gaaaaacccg gatgcgctgg agctgattcg
     4081 cggtaaatgc ggtcgggtgc agggggcgtt aaccggcatg atgatgacgc tgaaaggttt
     4141 gccgctggct tacaacaaag atatgcagga agacaaagaa ggtctgttcg acgcgctcga
     4201 tacctggctg gactgcctgc atatggcggc gctggtgctg gacggcattc aggtgaaacg
     4261 tccgcgttgc caggaagcgg cgcagcaggg ttactccaac gctactgaac tggcggatta
     4321 tctggtggcg aaaggcgtac cgttccgcga ggcgcaccat attgtgggtg aagcggtggt
     4381 ggaagccatt cgtcagggca aaccgctgga agatctgcca ctcagtgagt tgcagaaatt
     4441 cagcctggtg attggcgaag atgtctatcc gattctgtcg ctgcaatcgt gcctcgacaa
     4501 gcgtgcggca aaaggcggtg tctcaccgca acaggtggcg caggcgattg cttttg
//
LOCUS       DAOFSV010000242         4550 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.242, whole genome shotgun sequence.
ACCESSION   DAOFSV010000242 DAOFSV010000000
VERSION     DAOFSV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.242"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            96..254
                     /locus_tag="QBH27_004597"
     CDS             96..254
                     /locus_tag="QBH27_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDQ7016815.1"
                     /translation="METLSTNLQLARLVGVQGTPATIIGDEMIPGAVSWETLEAVVKE
                     KLAVAHAQ"
     gene            244..750
                     /locus_tag="QBH27_004598"
     CDS             244..750
                     /locus_tag="QBH27_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDQ7016816.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            933..1748
                     /locus_tag="QBH27_004599"
     CDS             933..1748
                     /locus_tag="QBH27_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDQ7016817.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2095..3981
                     /locus_tag="QBH27_004600"
     CDS             2095..3981
                     /locus_tag="QBH27_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDQ7016818.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4022..4549
                     /locus_tag="QBH27_004601"
     CDS             4022..4549
                     /locus_tag="QBH27_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDQ7016819.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
BASE COUNT         1095 a         1088 c         1297 g         1070 t
ORIGIN      
        1 gcagacagaa accgtaaaca ccggcattga catgccgggg aagcccttat agtacaataa
       61 ttctctatat ccaaactgac cccgatgaga aaagcatgga gacgttgagc accaatctgc
      121 aactcgcccg gctggttggc gttcagggga cgccggcaac catcattggc gacgagatga
      181 tccccggcgc ggtatcgtgg gagacccttg aggcggtggt gaaagaaaaa ctggcggtgg
      241 cccatgctca gtaaactgcg ccgctggctg cgtgaaggcg cgatcttact ggttgtgctg
      301 gctggcgtga taatcctgct ggatgtctgg cgttctccgc agatgccggc catgtttgac
      361 agcacgccgc tgcatacgct ggacggggaa accgtaacgc tggcgtcgat cagtgaagaa
      421 cgcccggtgc tgctctactt ctgggcgagc tggtgcggta tctgccgttt caccacgccg
      481 gatgtcgccc gtttgcagtc ggaaggggag agcgtcatga ccatcgcgct gcgttccggt
      541 aatgacgggg aggtttcccg ctggttatcg cgtaaaaggg tgacgttccc cgtcgtgaat
      601 gatagcggcg gtgagatatc acgaaactgg gaaatcagcg tcacgccaac gctggtggtg
      661 gtgtcgaagg ggcaggtggt aactacgacc agcggctgga ccagctactg gggcatgaag
      721 ttacgtctgt ggcgggcagc catgttttga tagcgtgccg gggaaacccg gcatttttta
      781 acccattgta aggatgtgta aaacgagcgg agagcgtgtt ttctgttatt tttgctgctt
      841 ttttaccgac gccagttatt attttttacc gctgcctgac gttgcaaacc tgaattgata
      901 atggcatatc tgttttaatt gaatgaaagt ctgtgatacg ttcaggcgat tttttcgatt
      961 tccattttga cagggataac aattcaatgc gcaagggtaa aattattgtt gcatcaatga
     1021 tggtgctttg tgtccttatt tttgttgcgg cggcggcatg gtttatgctg ttccagggag
     1081 aaatgacgga gccgacagag gaggaaaaac aggcggcaat tttacacgct gcgggcctga
     1141 tgaaatcaga gacccaggat aaaaaatcag tcgaaacgct ctatcatcgt tatattattc
     1201 aacgccatgt taatttagat tccggcgaac tggtggctgg cagcagcgcg gacaccgcgc
     1261 ggcagaagtg tgaaaaactt gcccctgaac gcgacccagc gcaggttcgt cagcgctgta
     1321 cggtggcgga tgtgttcttt gtgaaagata aaaataatga aattcagcag gttatcattc
     1381 cagttaccgg taagggtgcg aaatccatga tgcatgcgtt tcttgctctg gggctggacg
     1441 gtcgtacggt gagaaatctg tattattatc agcaacggga aacgccgttt ctgggggcta
     1501 gggtggaaga cgccaactgg cgcaaacagt ggccagggaa acgattatta gataacagcg
     1561 gacatcctgc attgaaaata gtacaggata aacctgaaca tgccgatgaa tataccgttg
     1621 acggtatttc cggtgcgacg ttgacatcaa ccggcgttga gaaaagtatt aactactgga
     1681 tggggccgca gggatatggt caattcttac agcgtcttgc cagcgatcga aataacctta
     1741 atctttgaat attgctgtac gcaataacgc gttttatttc tgcctgccga taatgaataa
     1801 tatcactgtc ggcaggggga tttattcctc tgttaccgcg cctgccttgc cgttaattcc
     1861 atgagaaaaa cagggagata acgtcccttt tatcaacgca taatgtgtgt gttcatcacg
     1921 atttgatgaa ataagtttac ataacttgtc gtctgctgac atttttatga tccaggtcat
     1981 gtataacgcg acaaaaagat atgataataa ttatcatttg tattctttgg cggtgtgcac
     2041 gtgcgtaact tccgtaactc tttgttggtt actctctttg gcttgtttat aagcatgagt
     2101 gtctgggcgt cgacgaatta tgcgcctttg attgaagata tcgagcaacg tcttgataaa
     2161 acagcggaac tttatcagca acagcatgcc gacgaggcgc gccgcacggt acaaatggcc
     2221 tattttgagg tgttcgagaa cctggaaggg ccgatccgca tcaacatttc cgcgcgcaaa
     2281 agttacgaga tggagagcgc attcggcgag atccgtcgaa tgattggcga gaaaaaaccg
     2341 ctcgctgacg tccaggcgcg cattgactgg ctgaaagccg cactgcgcga agtggaaccg
     2401 gtgctggatg gcggacatcg tctggtggcc gaagagcagc acaacgcgct gtccagggac
     2461 gacattgccg ttcactggca ggagagtttc aggacgatcg acgatctgct ggcgcaggcg
     2521 gtcactgaat atcaggccgg gaattacagc gttgccagtc agcacgttca acaggcgcac
     2581 tatcagggct ttaaaaactc tgagatggag atgtccctca gacaaaaccg ttctgcaaaa
     2641 gatgccgcct ccattaacca acaattctca tccctgattg cgctgaccgc gcaacctgac
     2701 catctcaacg atgtctccta tcaggtcact acgctgctac aggatatcga ggatatctta
     2761 cccggcttgc caaccacccg tgacgatcag caggttgccg cagcgcaaag cgcagacaac
     2821 attccggcgg cggacgaggg aaataaccgc accgactggg ctgaggtgac gacgggaatt
     2881 aaccagagca ttcaggacgc gatagcgcgc tatcagggcg gcgatgcgaa gaatgcgatt
     2941 ctggatatcc aggataccta cttcgaccgc ttcgaagcca gcggcatgga aaacaaaatc
     3001 ggctctcgcg attcggcatt taaaaccacg ctggaggcgt attttacccg cctggtcagt
     3061 ctgatgaagg ccggtcagcc ggttgagcgc ctgaatgcag aggccagcgc gctgacgcag
     3121 gatctgcaaa aagccgtgac gatgctgggc gaaggcgaag aaacccagtg gagtctgctg
     3181 ctctatagtc tgatgatcat cgtgcgcgag gggctggaag cgctgctgat tgttgcggca
     3241 atcgtggcct acatggtgaa aaacaaccat caggataagc tgccgcttat tcgtcagtcg
     3301 gtaattgtgg cgctgatcgc cagcgtcatc accgccgcta tcttccagat gctgtttact
     3361 aactccggcg ccagccgtga actgctggaa ggcataacga tgctgattgc cgtcgtgatg
     3421 ctgttcttta tgagttactg gctgctgtcc aaagtggaag cgcgccactg gaaagcctgg
     3481 ctggaaggca aactgtctca ttccctgtcg cgaggctccc tggtcggact atggctgacc
     3541 agcttcctgg cggtgtaccg cgaaggggcg gaaacggtgc tgttctatta cgctctgatt
     3601 ggcgatgcaa acacggtatc cggccatatg gcgattggcg ccggttttgt gattggctgc
     3661 gtggtgctgc tgctcgcctg gctggtgatg cgttactcag tggtgcgcct gccgctgaaa
     3721 cccttcttta tgtttaccgg cagtttcatg tatctgatgg ccttcgtctt tgcgggcaaa
     3781 ggcgttctgg aactggtgga aggcaaactg ttccagccta cgttgattaa cggcttccct
     3841 gaaatcagct ggctggggat ttatccctac gtggaaacgt tgttaccgca ggtcgtattg
     3901 ctgctcgcgg cgctggtggc gctgtgggtg atgcggcgca aaagcgcggt tcccggggag
     3961 acgataaaaa ataacccata atcgttgtat agccgttgtt ttagacgaga ggatacgttt
     4021 gatgaccatg aagaaaaccc tgattgccag cgcagtgatg gccagcattt tcattgcgcc
     4081 agccgcgttt gcttttaaag agtacccggc aggcgagcct gtcaccatga atgaaatgga
     4141 gctggcggcc gtttacctgc aaccgattga tatggaaccg cgcggcatgg gcttaccggc
     4201 agcaaaagcc gatgttcacc ttgaagcgga tatccacgct gtagaaggca ataaaaacgg
     4261 ctttggcgca ggggaatgga tcccgtacct gaccatcagc tacaccctgg tcaacaacga
     4321 cacgggcgaa aaacaggaag gcaccttcat gccgatggtt gccagcgatg gcccgcacta
     4381 tggcgcgaac atcaaaatga tgggcgtggg caactataaa gtgacctacc acattgaacc
     4441 gccgtcaaaa gccgggatgc atcgccatac tgacagcgaa acaggcgtag gccgctggtg
     4501 gaaaccgttt gatgtgagct acgagtttaa atatgtcggt ctgaactaaa
//
LOCUS       DAOFSV010000243         4478 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.243, whole genome shotgun sequence.
ACCESSION   DAOFSV010000243 DAOFSV010000000
VERSION     DAOFSV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.243"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(257..946)
                     /locus_tag="QBH27_004602"
     CDS             complement(257..946)
                     /locus_tag="QBH27_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ7016820.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(1057..1761)
                     /locus_tag="QBH27_004603"
     CDS             complement(1057..1761)
                     /locus_tag="QBH27_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7016821.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(1748..2584)
                     /locus_tag="QBH27_004604"
     CDS             complement(1748..2584)
                     /locus_tag="QBH27_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7016822.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(2577..3410)
                     /locus_tag="QBH27_004605"
     CDS             complement(2577..3410)
                     /locus_tag="QBH27_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7016823.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDIVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(3410..4459)
                     /locus_tag="QBH27_004606"
     CDS             complement(3410..4459)
                     /locus_tag="QBH27_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7016824.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
BASE COUNT         1128 a         1187 c         1150 g         1013 t
ORIGIN      
        1 cgcctgatgc gacgcttgcc gcgtcttatc aggcctacgc cagacagcgc aatagcctga
       61 tttggcgtga tttttgtagg tcggataagg cgtttacgcc gcatccggca tcaatgtctg
      121 atgcgatgct gacgtgtctt atcaggccta cgtcagacag cgcaatagcc tgatttggcg
      181 tgatttttgt aggtcggata aggcgtttac gccgcatccg acatcaatgc ctgatgcgac
      241 gtttgctgcg cctaccttaa ccgttagtta atttttcaaa ctcttcacaa ccggtatcaa
      301 atccttccat acagctcaga ttcaaccagt gcagcgcctt ttgtttattc ttctcgataa
      361 aaccctcttc accgtttaag aacatcattc ccgcccagta ttcggaataa ccggttcggg
      421 aaatcgcaga gctacgtttg aaataccagg ttgctttgtc atcgtcggcc ttaatgccca
      481 cgccgttggc gtaaatcaaa ccaagcagca tttgggcatc gacggcagag tcgttctcca
      541 gatcttccga ggcgttttct aacagcgaaa tcgcctttgg ataatccggt ttacccgctt
      601 gcgtatttac cagaatatgc gccagcgtta cttcaccttc tttactgccc gcttgcgctg
      661 ctttctcggc aagcactttt gcttgtggat agtccagact gaccggattg gtgattttga
      721 tctgcgccaa cagcgcgcag gcatcggcat caccgctgtc ggcggctttc tgtgcccaat
      781 attcggcttt gctcaaatcg ccggagctaa accagctgtc ggcaagaaaa tattgtgcgc
      841 gtcggtctcc ggcctctgct gcctttaaat actggctgcc aggctcggag tcgttcgcgt
      901 gggcaaagaa tgtcaaaaac aacatcaaag tgataatttt tttcattttg ataatgaagt
      961 ttggtagggg agagacagta taaagtgttc gggtggacaa aaaaacaact gtaaaaagac
     1021 ctgaaagcag aatgaattaa tgcaatgtca ggttttttaa tccatccgat gttccccctt
     1081 ttccatggcg tcgcgatcaa aaaatcgctg aatttttccc tcggacatca atgccgctcg
     1141 atcagacata tgcgcgatga catcggcatc gtgactgacc agcagatatg tcatttggtg
     1201 ttccagctta agttgattca acaaattaag gatttccgct tgtaccgaca tatccaacgc
     1261 cgaggtgggt tcatccagca gaagaagttg tggtcgtaac aacagggcgc gagcaataac
     1321 cacacgttgc cgttgaccac cggaaagctg gtgtgggtag cgacgagcgg cgtcgaaagg
     1381 caagccaacc tgttccagtg cagtctggac gcgtttttct atttctcttt cgccacgaat
     1441 tttgagtggt tcagccagcg ttcgccacag agtatgattg ggatgcaacg aggcataggg
     1501 atcctgaaac accatttgca cattgcggcg gagatcgccc tgaaaacgca ttcctggttt
     1561 gatggtgtgc ccaaacagtg acacctgacc gtgccagtcg cgctgtaatc cagcaattac
     1621 gcgcaaaatc gtggattttc cgcagcctga ttcgccaatt aaactaaatg tctctccggc
     1681 attaaccgaa aaactggcgg cagaaactgc ggttttctca gcaaaagtca cctgtaagtg
     1741 attaatttca acaatcgtca tggtatttct ccggcgtcat tgccgtcctg tccagcgtcg
     1801 gcagcatctg cccgtaagtt tgtgcatttg ggcggcaagt ccacagtgtg cgcgtgtaag
     1861 gatgcgttgc tgtaggtagc gcagcagcgt gcatttcatc caccttattt ccctgataca
     1921 tcaccagcac ccgatggcaa aattgggcaa ctaatggcag gtcgtgactg atcagcaaca
     1981 ttgccatctg acgttgttca cactgggtaa ccagcagttc cagaatctga ttacgcaggc
     2041 gcacatctag tgctgatgtt ggctcatcgg caatcaatac ccggggatcg ttgatgagcg
     2101 caattgcaat catcactcgc tgccccatgc cgccagaaag ttcacgagga tagctttgca
     2161 gtactcgcgt attgaggccg acagcctgaa ttgcggtgtt tattttctct tctttctcgc
     2221 ggcggttcag acgttgatgg agcgtaagcg cctcttccag ttgcgctttt actgtcttta
     2281 ctggattaag cgcgtaacgc ggatcttgca ataccatcgc gatatcgtta ccgcgcaact
     2341 gttgccatcc acgcgcgtta agggtgagtg catcacgccc cagaatgtta agtgtgtccg
     2401 cggttactac acccggttta cgcaccagcc ccatcagggc gcgggcggtc atggacttac
     2461 ctgaacccga ctcaccgacc agcgccagac gttcattgcc taatgtaaaa ctgacattgt
     2521 tgacgacccg ggactccgga taatcgacgc agagattgtt gatactaaga cgtgagtcag
     2581 tcatgttgag gctccagaac atcgcgcaag ccatcaccca gcagattaaa ggccagactg
     2641 gcaattaaga taattgcgcc gggagcggcg gcaatccacc attgatcaaa aatgacctgc
     2701 ataccgtcgg cgatcatggc tccccattcc gccattggcg ggcgagcacc aagcccaaga
     2761 aaacccagac cagcagccgc cagaatgata cccgctaaat ccagcgccag acggacaatg
     2821 gcagatggca ggcacaatgg caaaatatga ccagccagca gacgcctgcc gcggatgccc
     2881 atcatttctg cggcggcaag ataatcactg tgtcgcagac gttggatctc actccgtgcc
     2941 tgacgcgcat aagcaggcca ggtggttaat gcaagggcca gtgcgccgtt aaccagcccc
     3001 ggccccagca tggcaacaaa agcaaaagcc agaattaaac gcggcattga cattactatg
     3061 tcagtaaaac gcatcagcac gcgttccagc cagccgccgt agtacccggc taaaatgcca
     3121 atcagcagac cgaccgggag tgtaatgatt gtcaccaatg ccaccagccc cagtgccggg
     3181 cggctgccgt agattaaacg tgaaagtagg tctcgcccgt aactgtcagt gccgagccag
     3241 tgctgacgac tcggtgcttg taatcgcgcc gccgcatcct gccagttagg atcgtgaggt
     3301 gccagccacg gcgcgaagag cgcaattagc atcaataaca ggataattat caacccgcaa
     3361 aacgcggcgg gtgaatggcg taaacggcgt aaaaagagaa acatcggcat cagcgtaacc
     3421 tcggatcggt cagccggacc agcaggtcgg ttaagttatt tatcaacaca aagcagacgc
     3481 caatcagcag cgttcctccc atgattgctg tggtatcacc cgcgaagagc gctgtagtga
     3541 ggtagcgccc aatacctggc catgaaaaga cggtttctgt cagtaccgcg ccttccagca
     3601 ttgatgtata ggcgagggcg ataacggtga gcaaggtgcc gcgaatgttg ggtaaaacat
     3661 gacgtagcaa aattgtcatc tcgctggccc ctttggcgcg cgccagcaga atatattctt
     3721 tattcatttc gccaagacag gcggaacgag tcaggcgcgt aatactggcg agcgagaagt
     3781 acgccagtaa taaaaccgga agtatcaaat ggttgattgc attggtaaat gcctcgcgat
     3841 cgccagaaag ccacgtatca atcagcgcaa atccgctgcg gggttctatg gtgaattgat
     3901 aaatatcatc cagcctgccc ggcccggcac tccattgcag tctggcgtag aacagcgcca
     3961 gcatcagcag accaagccaa aaaacaggga cggaatttcc cagtagagtt aatgttcgca
     4021 cggtgaaatc cagcggagaa ccgacataac gggcgcaaag tacacccgca atgacgccga
     4081 caacagcacc gacaataagc gccaacgttg ccagttcaat ggtcgccggg aatgctgata
     4141 gcaagtcctg gagtactggt tgcccggtgg agctggcgat gcccagatcg ccgtgtgcca
     4201 gattcatcag gtaatgccag aactggacgg ctaacggctg atccaatcct aactggtggc
     4261 gtacctgatc ataggtggac tggctggcgt gatcgccaac gatttgtagt acgcgatcga
     4321 ccggtgagag agccgaaagc gcaaaggtaa tcagcagcag gccgagtagc gtcagagcaa
     4381 cggtaaacag cccttgcaac agcgcgagca gagggcgttt gcctcgccat cttgcgcgag
     4441 tggaaaaaac gacagacatg atctttccat acagtgat
//
LOCUS       DAOFSV010000244         4447 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.244, whole genome shotgun sequence.
ACCESSION   DAOFSV010000244 DAOFSV010000000
VERSION     DAOFSV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.244"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            341..4270
                     /locus_tag="QBH27_004607"
     CDS             341..4270
                     /locus_tag="QBH27_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_109867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ7016825.1"
                     /translation="MNRIYSLKYCHLTQGLIAVSELASRVSSKTGRKLLAIFIVSALS
                     YGAAEYACAAQMDTRNFWIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPD
                     FSPVSAKGATTAIGNAYSVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLD
                     KFVVETAGATEHADFNLSAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQTYN
                     PLLRSASMFQLNWNNMYASNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKV
                     FSSKDTWAFFARYDQNTVDILKNTFTQEVNLNGQKMTVNNKNIAINDKITAIELTKSN
                     KNKDLKFHGGGSIELTDNLNSGTGGLIFDEGQHYSVIGKDKAYKGAGVEIGKDTVVDW
                     SVKGEANDNLHKTGAGTLNVNVAQGNNLKTGDGTVFLNAEKAFNAIYVASGRGTVKLG
                     QADALDKNSDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTIITNTSDKTATLSLQN
                     PSRYVYHGSITGNTNIEHTGTQKSADSSLIIDGNINTRNDITVRNSQLRLQGHATSHA
                     IFREGPRHCYVPGVLCDKDYVTDFARLESEANKKNNSAYKTNNQVASFDQPDWETRHF
                     RFKTLNLENSEFTTARNSVVEGDIVASNSTLKLGGDVPVFIDMYDGINITGNGFGFRQ
                     DVREGRSADDGSSSYTGKITLQKGSTLDINNRFIGGIEAHDSKVNVTSPDALLQNSGV
                     FVNSTLSVRDGGHLTAQKGLYSNGRVQIGKKGTLSLSGTPENGADNTWMPVLTYMTEG
                     YDLTGDNATLNISQQAHVSGDVHATSSSSIRIGSENPGSVSSSVSPVLAAGLFSGYNA
                     AYYGAITGGKGNVSMNNGLWQLTGDSDINSLTTRNSRVQSEENGAFRTLTVKTLDATG
                     SDFVLRTDLKDADKISITEKASGSDNTLNVSFMKNPSPGQSLNIPLVSAPAGTSGDIF
                     KAGTRVTGFSRVTPTLRVDTTGGSTKWILDGFRTEADKAAAAKANSFMNAGYKSFMTE
                     VNNLNKRMGELRDTNGDAGAWARIMNGAGSADGGYSDNYTHVQVGFDKKHALDGVDLF
                     TGVTMTYTDSSADSDAFSGKTKSVGGGLYASALFNSGAYIDLIGKYIHHDNDYTGNFA
                     GLGTKHYGTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGEMDLSMK
                     NKDFSPLIGRTGIELGKTFRGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGE
                     KDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAINANFRYMF"
BASE COUNT         1275 a          930 c         1127 g         1115 t
ORIGIN      
        1 aatgatataa aataaaaaac aataaatata aaaacactta aacgtcatat gcttttgttt
       61 atttcatgat tttatttttg tagctttacc cttcattatg caatgtgggt atggagtcgt
      121 tattatattc taaataacct ttggcggcgc aatgcaattt aattaatgca atgtaaatat
      181 gctaaacaca ctgctataaa atctatattg tagatgtatt ttttacagac gtgggggggg
      241 ggttaaactg caattaataa aggtaattgg aagcttttta ttaataaaaa catagttcta
      301 ttggtttgta ttatgttata aacgttatgg agtcttaccc gtgaacagaa tatattcact
      361 taagtattgt caccttactc aagggcttat agctgtatca gaattagcat cccgagtatc
      421 ctctaaaacc ggtcgaaaac ttctcgctat atttatagtt tctgccttgt cttatggtgc
      481 ggctgaatat gcctgtgcag cacagatgga cacccgaaat ttctggatcc gtgattatct
      541 tgatctggca cagaataaag gtgcatttca gcccggagca tacggggtaa aaacgccatt
      601 aaaaaatggg ggagaattca gttttcctga agtaacaatc cctgattttt ctcctgtatc
      661 cgctaaaggt gcaacaactg ctattggtaa tgcctacagt gttacagcaa gtcataatgg
      721 cactattcac catgccatta aaactcagac atggggacag tcagattatc attatgttga
      781 tcgtgtgacc aaaggtgact ttgcggtcca gcgtctggat aagtttgttg ttgaaacagc
      841 gggtgcaaca gagcatgctg atttcaactt atcagcagca gaagcactag agcgttatgg
      901 tattgaattc aatggaaaga aacaaataat cggtttccgc gttggagctg gtgcaaccgg
      961 tgttacatct tatggggtgg gacagacata caatccatta ttacgtagtg cctccatgtt
     1021 tcagttaaac tggaacaata tgtatgcaag taataatacc ggtgggtttt ataatgaagt
     1081 gacaggaggt gacagcggtt ccggatttta cttgtatgat aatcagaaaa aaaaatgggt
     1141 cattttgggg acactgacag gtaaagtatt ctccagtaag gatacctggg ccttttttgc
     1201 tcgatatgat cagaacactg tcgatatcct gaaaaatact tttacccaag aggtgaacct
     1261 caatggtcag aaaatgacag ttaataataa aaatattgcc attaacgata aaataactgc
     1321 tattgaactg accaagagta ataaaaataa agatttgaaa ttccatggtg gcgggagcat
     1381 tgagctcacc gataacctga actcaggaac cggaggattg atttttgatg agggacaaca
     1441 ttattcggtt attgggaaag ataaagccta taaaggggcg ggtgttgaga tcggaaaaga
     1501 tacggttgtc gactggtcgg taaaagggga ggcaaacgat aacctgcaca aaacaggggc
     1561 cgggacactg aatgtcaatg tggcccaggg gaataacctg aaaacaggtg acggtaccgt
     1621 ttttcttaat gcagaaaagg ctttcaatgc tatctatgtt gccagtggcc gtggaacggt
     1681 caaactgggg caggccgatg cgctggataa aaatagtgat tacagaggta tttattttac
     1741 tagtcgtggg gggactctgg atttaaacgg gttcagccag tcgttcaaga agatcgcggc
     1801 aactgatgtt ggtaccatta tcaccaatac ttctgataaa acagcgaccc tttccctaca
     1861 aaacccctcc cgctatgtct atcacggtag tatcacggga aatacgaata tcgaacacac
     1921 tgggacacag aaaagtgctg acagcagtct gattattgat ggaaacatta atacacgcaa
     1981 tgacattact gtgcggaatt cccagctcag acttcagggg catgccacat cacatgcaat
     2041 attccgcgag gggcctcggc actgctatgt ccccggagtt ctttgtgaca aagattatgt
     2101 tactgatttt gccagactgg aaagtgaggc aaataagaaa aataacagtg cctataaaac
     2161 aaataatcag gtggcttctt ttgaccaacc tgactgggaa acccggcatt tccgatttaa
     2221 gactctgaat ctggaaaact cagaattcac aactgcacgt aactcagttg ttgagggtga
     2281 tattgtcgca tcgaattcaa cgctgaaact ggggggcgac gttccggtgt tcattgatat
     2341 gtatgatggc atcaatatca ccggtaatgg ttttggcttc cgccaggacg ttcgtgaagg
     2401 acgctcagca gatgatggca gtagcagcta tacaggcaaa attacactgc aaaaaggctc
     2461 cacgctggac atcaacaacc ggttcattgg cggtattgaa gcccatgaca gtaaggtaaa
     2521 cgtcacctca ccggatgccc tgctgcagaa cagtggtgtt ttcgtgaatt ccaccctttc
     2581 tgtccgtgac ggcggtcatc tgacggcaca aaaagggctc tacagtaacg gccgggttca
     2641 gattggaaag aaaggtacgc tttccctgag cggcacgccg gaaaatggcg ctgataatac
     2701 ctggatgccc gtactgacat acatgacaga aggctatgat ttaaccggtg ataacgctac
     2761 gctgaacatc agccagcagg cgcatgtttc cggggatgtt catgcaacca gttcatccag
     2821 cattcgtatt ggctccgaaa accctggctc agtttcctct tctgtctccc ctgttctggc
     2881 tgccgggttg ttcagcggat ataacgcggc gtactacggt gccatcaccg gcggtaaggg
     2941 gaacgtcagt atgaataatg gcctgtggca gctgaccgga gattccgaca tcaacagtct
     3001 gacgacccgt aacagccggg tacagtctga agaaaacggt gccttccgta ccctgacggt
     3061 taagacactt gatgccacgg gcagtgattt tgtcctgcgc actgatctga aggacgctga
     3121 taaaatcagt attacggaga aagccagcgg ttcagacaac accctgaatg tcagctttat
     3181 gaagaacccg tctccgggac agtccctgaa tatcccgctg gtcagtgcac cggccggaac
     3241 atcaggggat atctttaagg ccggcacccg ggtgacaggc ttcagtcgtg tgacgccgac
     3301 gctgcgtgtt gacaccactg gcggcagtac gaagtggatt ctggatggtt tcaggacgga
     3361 agctgataaa gcggctgcag cgaaggcgaa cagtttcatg aatgccggct acaaaagctt
     3421 tatgacggaa gtaaacaatc tgaacaaacg tatgggggag ctgcgtgaca cgaacggtga
     3481 tgccggtgcc tgggcccgta ttatgaacgg cgccggttca gccgatggcg gatacagtga
     3541 taactacacc cacgttcagg tcggttttga caaaaaacat gcgctggacg gtgttgacct
     3601 gttcaccggt gtcacgatga cctataccga cagcagtgca gacagtgatg cgttcagcgg
     3661 gaagacaaaa tccgtggggg gaggtctgta tgcttcagca ctgtttaact ccggtgccta
     3721 cattgatttg attggtaaat atattcacca tgacaatgac tacacaggta actttgccgg
     3781 tctggggacg aagcactacg gaacccactc ctggtatgcc ggagcggaaa cgggttaccg
     3841 ttatcacctg acggaagaca catttattga gcctcaggcc gaactggttt acggcgcagt
     3901 gtccgggaaa acattccgct ggaaagacgg tgagatggac ctgagtatga agaacaagga
     3961 tttcagcccg ttgattggca gaacagggat tgagctgggc aaaaccttca ggggtaagga
     4021 ctggagtgtg acagcccgtg ccggaaccag ctggcagttt gacctgctga ataatggcga
     4081 gacagttctt cgtgatgcat ccggagagaa acggatcaaa ggggagaaag acagcaggat
     4141 gttgttcaat gttggcatga acgcacagat aaaggacaac atgcgctttg gtctggagtt
     4201 tgagaaatcc gcctttggta aatacaacgt ggataacgca ataaacgcga atttccggta
     4261 tatgttctga caaggagagg cccctgaaga ggggcctgta gtgatgttac tcccgggtta
     4321 atgcagctag taaaggtatg gctctgacta ccctggaaag tggtgcccat cgcatcacta
     4381 caaattctgg caattgcgta cgtggtaacg gcaagttatt taacctcaaa cgtacgtgcc
     4441 cgagcgg
//
LOCUS       DAOFSV010000245         4403 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.245, whole genome shotgun sequence.
ACCESSION   DAOFSV010000245 DAOFSV010000000
VERSION     DAOFSV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.245"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..600)
                     /gene="kdgA"
                     /locus_tag="QBH27_004608"
                     /gene_synonym="eda"
     CDS             complement(<1..600)
                     /gene="kdgA"
                     /locus_tag="QBH27_004608"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDQ7016826.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPIAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRI"
     gene            complement(637..2448)
                     /gene="edd"
                     /locus_tag="QBH27_004609"
     CDS             complement(637..2448)
                     /gene="edd"
                     /locus_tag="QBH27_004609"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDQ7016827.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(2683..4158)
                     /gene="zwf"
                     /locus_tag="QBH27_004610"
     CDS             complement(2683..4158)
                     /gene="zwf"
                     /locus_tag="QBH27_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDQ7016828.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
BASE COUNT         1069 a         1206 c         1059 g         1069 t
ORIGIN      
        1 aatgcggtcg taatcgcccg cttccagcgc atctgccgga accagccagg aaccaccgat
       61 gcacagcacg cttttcagcg ccaggtagtc acggtagtta gccggagaaa taccacctgt
      121 cgggcagaaa cggacctggg agaacggacc cgcgatcgcc tgcagggctt tcacaccgcc
      181 gttagcttca gccgggaaga atttgaactc tttcaaaccg tagtccatac ccagcatcag
      241 ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc ccttcggtag cagctttcag
      301 cagcggctcg gtcagacccg ggctaattgc aaactgtgca cctgcttcag tgacttctgc
      361 cagctgctgt ggattcagca ccgtaccggc acccacaatc gcttcaggca cttctttggc
      421 gatagcacgg atagcgtcaa ctgcgcactc ggtacgcaga gtcacttcca gaacgcgcac
      481 cccaccagca accaacgctt ttgctatcgg caccgcgtgt tccagttttt ttaccacgat
      541 aaccggtaca accgggccgg tggtcaggat tgattctgca cttgttttcc agtttttcat
      601 cagagttttc tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac aggttgcgcc
      661 ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc ctgttcccac
      721 gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg cttcgtctac
      781 cagcagcgtc agttcgcctg tctgtccatt cacacgaatg atgtccccgt cgcgcacttt
      841 tgccagcagc ccgccatcgt aggcctctgg tgttacgtgg atagctgacg gcactttacc
      901 tgaagcgccg gagagtcgtc catcggtaac taacgcaatt ttgaaacacc ggtccaataa
      961 tacaccaagt ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt ttggcccctg
     1021 atgacggaca acaacgacac aatcgcggtc cagtaaaccc gcttcaaagg ccggcataac
     1081 gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc acctggttct ctaccggcac
     1141 ggcagaggtt ttcataaccg ctcgccccag gttaccgctt aacacttttg tcccaccatg
     1201 atgagagaaa ggttgttcga aggacgcgat cacattgttg tcgagtgatt tttctgcccc
     1261 ttcccgccag tccagttcac cattattcag ccatggttca agggtataac gagacagacc
     1321 aaagcctgcc accgtattga catcttcatg cagcaggcct gctttgagca gttcacgtac
     1381 cagaaccggt acgccacctg ccgcctggaa gtggttaata tcggccggac cgttcgggta
     1441 gagacgtgcc atcagcggta caacatcaga aaggtcagag aagtcatccc agttaatctg
     1501 aataccggct gcgcgcgcca tcgccactag gtgcatggtg tggttagtgg aaccaccggt
     1561 cgccagcagt gcaacgatac cgttcaccac cactttctca tcgatcatct taccgatcgg
     1621 catccattca ttaccattac cggtcatgcg cgtaacctgg cgcgcagctg cagcggtcaa
     1681 agcatcgcgc agcggagaat ccggatgaac aaaagaagag cctggcaact gcatccccat
     1741 aaactccacc accatctggt tggtgttggc agtaccgtag aaagtacatg ttcccggcgc
     1801 atgataagac gcggcttctg actccagtaa tgccatgcgg tccactttac cttcggcata
     1861 aagctggcga atacgcactt tttctttatt tggcaaaccg cttgccatcg gtccagacgg
     1921 cacaaacacc gcaggcaaat gaccaaacga cagggctgcc atggtcagac ctgggacaat
     1981 cttgtcgcac acaccgagaa acagagcacc atcaaacatg ttatgggaca gccccaccgc
     2041 cgcagacatc gcaatcactt cgcggcttaa cagcgacaat tccattccat cctgcccctg
     2101 ggtgacacca tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg cattcgcttc
     2161 atgcagggct ttacgaatga tttctggata gtgttcataa ggctggtgag cggagagcat
     2221 gtcgttatag gaggtgatga tggcgatatt gttacgcaac atgcttttca aagaggcttt
     2281 gtcttctggc tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca actgcgaacg
     2341 atgaacggtc gaagttttcg cttgttctat ccgggcgagg taagcagagc gagtctcgcg
     2401 cgaacgttca atgattcgat ttgttacgcg taacaattgt gggttcataa aggctcctga
     2461 aattgagttg tcagagcagg atgattcaca acgcgtttca tacatggaaa ttatgattga
     2521 aacgcctgta accggagctc atagggcaaa aacgtttcag tcagtgtaat aaaaaaagcc
     2581 tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt gttctacaat ctgtgcaaga
     2641 tcatgttacc ggtaaaataa ccataaagga tgagcgcaga tattactcaa actcattcca
     2701 ggaacgacca tcacgggtaa tcatcgccac tgaggcaacg ggtccccagg ttccggcctg
     2761 atacggtttc ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt ctacccattt
     2821 ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc tgaataccac gcatggtttc
     2881 cagcagcaaa cgttcatagg catccgccag atgcgtctga ttaaaggttt ctgaatagct
     2941 cagatccagc ttggtgattt gcaggttatg tttgtggtca aggccaggaa ctttattcag
     3001 tacctggata tccacgcctt catcaggttg cagacggata gtcagtttat tttgcggtag
     3061 atcctgccac gactctttaa acagattcag ttcaggtgtt ttgaaataga ccacgacttc
     3121 agaacatttg gtcggcagac gtttaccagt acgcaggtag aatggcacac cggcccagcg
     3181 ccagttatca atgtcgacgc ggatcgccac gaaggtttct gtattgctgc tcttgttcgc
     3241 gccctcttct tccagatagc ccggcacttt tttgccctga gcgaagcccg cagtatattg
     3301 cccgcgtacg gttttttcgc gcacattgga gcggtcgatg cggcgcagag acttcagtac
     3361 tttcactttt tcatcgcgga tgctgtctgc gctcaggtca gacggcggag acatcgcaat
     3421 catgcaaaga atttgcagca ggtggttctg gatcatgtcg cgcatctgac cggctttatc
     3481 aaaatagccc cagcgccctt cgatccccac ttcttctgcc acggtaatct caacatgatc
     3541 aatggtgcga ttgtcccagt tgttcacaaa cagagagtta gcaaaacgca gcgccaacag
     3601 gttcagcact gtttctttac caagatagtg gtcgatacgg taaacctggc actcctcgaa
     3661 gtattcgcca acctgatcgt tgatttcctg cgaggtcgcc agcgacgtcc ccagcggttt
     3721 ttccatgact acgcgtgccg gtttagcatt cagttttgcc tcaccaagcc ctttgcaaat
     3781 tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaatg gtgatacgat ttttttgatc
     3841 cagcatcgcg ccgagacggc tgaatgcagc agtgtcattg acatcaagat tacaaaaatc
     3901 cagacgtgca ctcagggtgt cccataaacc ttcatcaatg gtttctttca tgaaagtttc
     3961 gagcgcctcg cggacaactt tggtataagc cgctttatcc cagtcagcac gccctacgcc
     4021 gataatccgg gtgtccgggt tgagctgacc ggctttttcc agttgataca gggaaggcag
     4081 caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac aggcctgggc
     4141 tgtttgcgtt accgccatgt cattctcctt aagttaacta acccggtact taagccaggg
     4201 tatacttgta attttcttac ggtgcactgt actgctttta cgagcttgcg aaaactgtaa
     4261 acgcttatcc acccgtgcga ttacggtaaa agcgcgcaaa gtgcggcaaa actgataaaa
     4321 aaatcatcgt tttccattgg gtaaaaatct gacactgatc atgttatgaa aaaaaataac
     4381 aactttttta tctgcttttg tca
//
LOCUS       DAOFSV010000246         4403 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.246, whole genome shotgun sequence.
ACCESSION   DAOFSV010000246 DAOFSV010000000
VERSION     DAOFSV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.246"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            802..999
                     /gene="fimB"
                     /locus_tag="QBH27_004611"
                     /pseudo
     CDS             802..999
                     /gene="fimB"
                     /locus_tag="QBH27_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            complement(1030..2250)
                     /locus_tag="QBH27_004612"
     CDS             complement(1030..2250)
                     /locus_tag="QBH27_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="HDQ7016829.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLT
                     CPTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVD
                     EVAFKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAG
                     YIRAARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISLAS
                     ECDVPMMKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAY"
     gene            complement(2269..2787)
                     /locus_tag="QBH27_004613"
     CDS             complement(2269..2787)
                     /locus_tag="QBH27_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDQ7016830.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKG
                     TEMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSEL
                     SYELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGRL
                     RKWAKNNNISLK"
     gene            2887..3294
                     /gene="fimB"
                     /locus_tag="QBH27_004614"
                     /pseudo
     CDS             2887..3294
                     /gene="fimB"
                     /locus_tag="QBH27_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            3772..4368
                     /gene="fimE"
                     /locus_tag="QBH27_004615"
     CDS             3772..4368
                     /gene="fimE"
                     /locus_tag="QBH27_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDQ7016831.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         1218 a          909 c          944 g         1332 t
ORIGIN      
        1 cttggtgaaa ataagtgttt acccgccatc aggctgagca taattctcat catgaaatat
       61 gtttcttggt ttgtggcttg taactggtca ctcctgatgt cgatctggag aggcttgttg
      121 atgctggtgt tttcaggatg atgtttcaat tagtttgttt gccatatcgc ccggcgagta
      181 gctgtgattg aggagggttt aagtcgtagt gaccaaagcc atatttacca acggatgtag
      241 atgaaaaagc gtcgcctgcg ttcccccata tctccaggat aaaaaggaat gtaacaatct
      301 catgacctaa actgacgaac cagccaggaa taatcgctag ggacctaaga attagcttga
      361 taatagccac taagaaatta ctgcgttcca tgaaatagcc attttgtggt aaatggagtt
      421 gactaataat gtcatatgtg agacggctag ttgaacgaat attaaatttt gctgaatttt
      481 ttatgttgat tttacttgtt acagaacata tcacatgata tatagataag attagttgca
      541 ttaatgatga gggttattat tagattcgta tccgattgat aaatatataa aggtacatag
      601 catgcaagag catggcgttt gtatggcaaa gttattataa gtaactatcg ctactccatt
      661 taagttcact cagaagaact ggtccactta cgttagttat taagcaaacg ttcgctttta
      721 taaacataat caggataaaa atgttggatt attgctaacc cagcacagct agtgcgcgtc
      781 tgtaattcta agggaaaaac gatgaagaat aaggctgata acaaaaaaag gaacttcctg
      841 acccatagtg aaatcgaatc actccttaaa gcagcaaata ccgggcctca tgcagcacgt
      901 aattattgtc tgactttgct ttgttttatt catggtttcc gggcgagtga aatttgtcga
      961 ttgaggattt cggatattga tcttaaggca aagtgtatat agggtcttcc ccgatcatgg
     1021 tgggaagact cagtacgcca tattcagttt cccatagtgg aacataactc ccagtttaaa
     1081 gcgttcccgg tttcggtatc ccctggcctt tatcctcagc agtctgatct tgctgttcag
     1141 cgcctccgca tttccattcg agacgcgatg acgcattgca ttcagtattc cgtataaccg
     1201 ttttttaatg gttttcgctg cgtttttcat catcggcaca tcacattcag acgccagcga
     1261 tatccactgc aaccagtcat tcctgcgttc tgtgctccag gggcgatccc agatattttt
     1321 tgccagctct ttcagtgtcc agcactggct tgtctgttgc atctgttccc gcaaccacat
     1381 cagcttttcc tgccgggact cagtcatcca tttgtcgctg tactgccaca ggaagcgggt
     1441 acctttggcc tgacgacgac tttcaacagg gaggtgcgga tgttcattct ggcgggtttt
     1501 atcaaccact tcgcccagtt gcttcgccac atggaagcga tcgaaggcga ttttctcgac
     1561 cgcattgggt aaatggatac gcgctgctct tatatagccc gcattcatgt ccatcgacag
     1621 tgttttgatg gccaacaact gactgtcagt aagcgaacgg agatagctgg caagactctc
     1681 tgtgccacga tcatcggtta atgccagtgc ccgcccgtcg cgatcagata ccacggttat
     1741 atacctatgc ccttttttaa aggcgacttc gtctacattc atatgacgca ctgataaagg
     1801 ctttttaatc cgcgacaggc ctcgcttaac cgcgcgggtc atgatgccgt caacggcatt
     1861 ccagctaagt ttaagttgtt ttctgaccgc atcaacggtg ctgattttaa gccatgagag
     1921 cacgaacgat tcgaacagca gagtgtaccg actgccggaa cctgcccacg gtacaggcag
     1981 agtctggcag ccatgctccg ggcacataac gcggggaaca tcggcttcta ctaatgtcat
     2041 gaactggcag gtatcaaggt ggcgccattt acgatgtcgg tgatcgtgaa cagaacagga
     2101 tttcctgcag gtcggacagg ttaactgagt attttcagca attccgactg taacagtaac
     2161 ggaacctgca ttttcatcga gggtaaggga tttaacctgc catggtgcag tcaggttaag
     2221 gatatgggca tagagggatt tttcgtccat ataatttcat tctgaagctt actttaaact
     2281 gatattatta ttttttgccc actttcttaa tcttccatta gcgttggcgt aaggcgaaga
     2341 tgtgttaaat gagatcattc tccccatcgt ccattttgtg taccatggtc ttccatacaa
     2401 aatatcatcg gtacgatcat taataaggtt cacaatctca ttttttacag tctgaagttc
     2461 agctactaac aactcataac ttaactcact gtaatctgag taaaattttt gagcaagaag
     2521 gccaagctga ttccatttat agccagtttc cggaaaatcg acaggcagac ctttagcatc
     2581 agaagcgatc cactttacaa caagagcatt ccatccaagc agatacgaaa cgagatcacg
     2641 aacactcatc tccgttcctt tggcgtgtcc gtccattgat ttatctgaag taatttctgg
     2701 tgggattgtg ttgaggtaac taattaattt actaaaattt ttatcaatag ctaaaagcag
     2761 ttcagctttt gtttgcggca cactcataag aattccctct catgtaaaaa gagacaccat
     2821 aaaggtactg tgtggtgttt gaaaatcact caattatgta ccacccacca caacagggga
     2881 agacccatat atatccatcg attaaaaaaa ggcttttcaa caacgcatcc gctattgaac
     2941 aaagaagttc aggctttaaa aaactggttg agtatccgta cttcgtaccc gcatgctgag
     3001 agcgagtggg tatttttatc tcgtaaggga aatccgcttt ctcggcaaca gttttaccat
     3061 attatctcga cttccggtgg taatgccggg ttgtcactgg agattcatcc gcacatgtta
     3121 cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact tatccaggat
     3181 tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa tgcagggcgt
     3241 ttttacggca tctgggatag agccagagga cgacagcgtc acgctgtttt atagagatag
     3301 ttgagatacc agggatggtg tttaaaacta cattattctt acgatatatc aaaaatgatt
     3361 aaccatttat taattgatgt tattgtaagt gtgattatta attttcataa taaacattaa
     3421 gttaaccata tccatacaaa atacaatggt ttatgttctt caaaataaat aaacaaaatc
     3481 attcataaat ttacacatca cttaaattct cctgtttccg cacttttttc tttatttttt
     3541 aagcaactgg aagttaatcc actgcaatct attgttatat tgaatcaaat caatgaaaat
     3601 agatgttgtc acatcagtga tattttattt ttgtatgata tttaatgtaa ttgactgata
     3661 gccacatcac tccgtgtgtg gttatctttt tatctattgg gctaattttg accgattgag
     3721 gtttcctata ggtattcatt caaatatatc tcagttagga gtactactat tgtgagtaaa
     3781 cgtcgttatc ttaccggtaa agaagttcag gccatgatgc aggcggtttg ttacggggca
     3841 acgggagcca gagattattg tcttattctg ttggcatatc ggcatgggat gcgtattagt
     3901 gaactgcttg atctgcatta tcaggacctt gaccttaatg aaggtagaat aaatattcgc
     3961 cgactgaaga acggattttc taccgttcac ccgttacgtt ttgatgagcg tgaagccgtg
     4021 gaacgctgga cccaggaacg tgctaactgg aaaggcgctg accggactga cgctatattt
     4081 atttctcgcc gcgggagtcg gctttctcgc cagcaggcct atcgcattat tcgcgatgcc
     4141 ggtattgaag ctggaaccgt aacgcagact catcctcata tgttaaggca tgcttgcggt
     4201 tatgaactgg cggagcgtgg tgcagatact cgtttaattc aggattatct cgggcatcga
     4261 aatattcgcc atactgtgcg ttataccgcc agtaatgctg ctcgttttgc cggattatgg
     4321 gaaagaaata atctcataaa cgaaaaatta aaaagagaag aggtttgatt taacttattg
     4381 ataataaagt taaaaaacaa ata
//
LOCUS       DAOFSV010000247         4377 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.247, whole genome shotgun sequence.
ACCESSION   DAOFSV010000247 DAOFSV010000000
VERSION     DAOFSV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.247"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..75
                     /locus_tag="QBH27_004616"
     CDS             <1..75
                     /locus_tag="QBH27_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDQ7016832.1"
                     /translation="LAYEGSGSGDWRTDAFIVGIGYRF"
     gene            227..3052
                     /locus_tag="QBH27_004617"
     CDS             227..3052
                     /locus_tag="QBH27_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ7016833.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASDASASASEAATHATDAAASSRAASTSAG
                     QAASSAQSASSSAGTASTKAREAAKSAAAAESSKSAAATSASAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGKATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALT
                     GTPTTPTARQGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTM
                     TSALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVI
                     EYLGLRELGTSGEKIPLLSTANTWTNRQTFSGGLSGELSGNASTAAKLKTARKISNVA
                     FDGSSDITLKASHVGAFALGKTGSTVANDKAVGWNWSSGAYNATISGASTLIIHFYMG
                     EGSCPAAQFRINYKNGGIFYRSARDGYGFEADWSEFYTTTRKPSAGDVGALPLSGGQL
                     NGALGIGTSSALGGNSIVLGDNDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTIN
                     ITGRVNPSDYGNFDSRYVKDVRLGSQQYYGVNNWQTWNFQCPSGHVLSGINVQDTGSN
                     SADNIAGVYYRPVQKYINGTWYNVASV"
     gene            3054..3587
                     /locus_tag="QBH27_004618"
     CDS             3054..3587
                     /locus_tag="QBH27_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ7016834.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGYSVVEIPDITANRRADDSGKWMFKDGAVI
                     KRVYTEEELRLQTENQKKILLQQAREKTQFWQTQLTLGIITDSDRQQLMNWMRYVQQV
                     ETTDTSVLPVTFPEPPE"
     gene            complement(3618..4145)
                     /locus_tag="QBH27_004619"
     CDS             complement(3618..4145)
                     /locus_tag="QBH27_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ7016835.1"
                     /translation="MQHLKNITAGNPKTIEQYQLTKKAGVIWLYTEDGKNWYDELKNF
                     QDDTLKIAYDQKGIIRCIEKDVSTLNPDGLSVVELPNITANRRADISGNWKFLDGKVV
                     KREYTKQELQQQAELQKAALLSEAESVIQPLERAVRLNMATDEERTRLESWERYSVMV
                     SRVDTAKPEWPQKPE"
     gene            complement(4161..>4377)
                     /locus_tag="QBH27_004620"
     CDS             complement(4161..>4377)
                     /locus_tag="QBH27_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7016836.1"
                     /translation="LNDYLNSSFIRNVRLGGRRSDTLYRGGLCEPGNGHVTTGLQIIG
                     EVDGDDWMVSRPLQKYISGNWYNVEQA"
BASE COUNT         1197 a         1056 c         1104 g         1020 t
ORIGIN      
        1 cttgcttatg aaggttccgg tagtggcgac tggcgaacgg atgcatttat tgttggtatc
       61 ggataccgtt tctgacaaca gacgccgatt tatcttctgt aaatattgtt atgatacgca
      121 ggttcatcca ccttatgggg tgaactgcgt ttgaggaaac gtaaagttac actgtcctga
      181 agcccgtggc gtcactgctg cgggcttttt ttattggtgg aaaagtatga cagtaaaaat
      241 ttctggcgtg cttaaagatg gcacaggaaa accagtacag aactgcacca ttgtgctgaa
      301 ggccagacga accagcagca cggtggtggt gaacacggtg gcctctgaaa atccggatga
      361 agccggacgt tacagcatgg atgttgagca tggtcagtac agcgtcaccc tgctggttga
      421 aggttttccg ccttcacatg ccgggaccat taccgtctat gaaggttcca gaccaggtac
      481 gctgaatgat tttctcggtg ccatgacgga ggatgatgta cgaccggagg cactgcgccg
      541 ctttgagcag atggtagaag aggtgtcacg taacgcctcc gcggttgcac agaatacggc
      601 agccgcgaaa aaatcagcca gcgatgccag tgcatcagcc agcgaggcgg caactcatgc
      661 aaccgatgct gcagcctcat cacgtgccgc cagcacgtca gccggacagg ccgcgtcgtc
      721 ggctcagtca gcgtcttcca gcgcaggaac ggcatcgaca aaggcccgtg aagcagcaaa
      781 aagtgctgct gctgcagagt catcaaaaag cgcggcagct accagcgcca gtgccgcgaa
      841 aacgtcagaa acgaatgccg cagcatcaca aaaatcggca gccacttctg catccacagc
      901 gaccacgaag gcgtcagaag ctgccacctc ggcacggggt gcggcggcct caaaagaggc
      961 agcgaaatct tcagaaacga atgcatcatc aagtgccagt agtgcagctt cctcggcaac
     1021 ggcggcagga aattccgcga aggcggcaaa aacgtccgag acgaacgcta agtcttctga
     1081 aacagcagcg ggacagagcg cctcagctgc ggcaggttca aaaacagcgg ctgcattatc
     1141 tgccagtgcc gcgtcaacaa gtgccgggca ggcctcagcc agtgccaccg ccgccggaaa
     1201 atcggcagaa agcgccgcat catccgcttc aacagccaca acgaaggctg gcaaagccac
     1261 tgagcaagcc actgcagcag cgaggtctgc ttctgcagca aaaacctctg aaacaaatgc
     1321 aaagacttca gcagacaatg ctgcttcctc taaggcggca gccgcatcgt cagccagttc
     1381 agcggcgtca tcggcatcat ctgcgtctgc ttcaaaagat gaggcgacca gacaggcgtc
     1441 agcagcaaag ggcagcgcca cgacggcatc cacgaaggcg acagaggcag ctggcagtgc
     1501 gacggcggca gctcagagca aaagtacggc ggaatccgcg gcaacgcgcg ccgagacagc
     1561 ggcaaaacgg gcagaggata ttgcatccgc cgtggcgctt gaggatgcga gcacgacgaa
     1621 aaaggggata gtacagctca gcagtgcgac caacagcact tccgagtcac tggcggcaac
     1681 gccaaaagcg gttaaggcgg taatgggtga aacgaacaag aaagcgccct taaatagtcc
     1741 tgcactgacc ggaacgccaa caacaccaac tgcgcgacag ggaacgaata atacccaaat
     1801 cgcaagcacg gcttatgtta tggctgcgat cgctgccctc gtggactcgt cgcctgacgc
     1861 actgaatacg ctgaacgagc tggctgcggc gttgggcaac gacccgaatt ttgcgaccac
     1921 catgactagc gcgcttgcgg gtaagcaacc gaaagatgcc accctgacgg cgctggccgg
     1981 gcttgctact gcggcagaca ggtttccgta ttttacgggg aatgatgttg ccagtctggc
     2041 aaccctgaca aaagttgggc gggatattct tgcgaaatcg accgttgccg ccgttatcga
     2101 atacctcggt ttacgagaac tcggcacaag cggggagaaa ataccgttac tcagtacagc
     2161 gaatacctgg actaatcgac aaacattcag cggtggcctt tctggtgaac tatccggcaa
     2221 tgcttctaca gctgcaaaat taaaaactgc caggaaaata agcaatgtgg cttttgatgg
     2281 ttcctccgat atcacattaa aagcaagtca tgttggtgcg tttgccttag ggaaaacagg
     2341 aagcaccgtt gcgaatgata aagcagttgg atggaactgg agtagcggag cctataacgc
     2401 aactattagt ggtgcatcaa cgttaattat tcatttttat atgggagaag gaagttgtcc
     2461 tgcagctcag tttcggatta attataaaaa tggcggtatt ttttatcgtt cagcccgtga
     2521 tggttatggt tttgaagccg actggtccga attttacacc accaccagaa agccttcagc
     2581 aggagatgtt ggtgcactgc cgttatctgg tggtcaactg aatggtgcac tgggtatcgg
     2641 aacatccagt gctcttggcg gtaattcgat tgtattgggt gataatgaca cgggctttaa
     2701 acaaaatggc gatggtaatc tggatgttta tgctaataac gtccatgtta tgcgctttgt
     2761 ctccggaagc attcaaagta ataaaaccat aaatattaca gggcgtgtta acccctcgga
     2821 ttacggtaac tttgattccc gttatgtgaa agatgttcga cttggttcac agcaatatta
     2881 tggagtgaac aactggcaaa catggaattt ccagtgccct tcaggtcatg tattgtctgg
     2941 tattaatgtt caggatacag ggtctaactc tgccgataat atagcgggcg tttattacag
     3001 acccgttcaa aagtatataa atggcacctg gtataatgta gcgagcgttt aatatgatgc
     3061 acttaaagaa cataaaagcg ggtaatgcta aaacactgga acagtatgag ttaacaaaga
     3121 aacacggagt tatctggctt tactctgagg acggaaaaaa ctggtatgag gaagtgaaaa
     3181 actttcagcc agacaccata aagattgttt acgatgaaaa taatattatt gttgccataa
     3241 ccaaagatgc ctccacactt aaccctgaag gttatagcgt cgttgagatt cctgatatta
     3301 ccgccaaccg gcgtgctgac gactcaggta aatggatgtt taaagatggc gcagtcatta
     3361 aacgtgtata taccgaagaa gagttgcgcc tacagacgga aaaccagaaa aaaatattat
     3421 tacagcaggc cagagaaaaa acgcagttct ggcaaacaca gcttactttg gggattatca
     3481 ctgattcaga cagacagcaa ctgatgaact ggatgcgata cgtgcaacag gttgagacaa
     3541 ctgatacctc ggttcttcct gtaacattcc cggaaccacc agaataagaa aaggcccgta
     3601 cgggccttaa tttgttgtta ttctggtttc tgtggccatt caggctttgc agtatccaca
     3661 cggctgacca taacgctgta gcgttcccat gattccagtc gtgtgcgttc ctcatcagtt
     3721 gccatattca gtctgacagc acgttccagc ggttgaatca cagactccgc ttcggaaagc
     3781 aaagcggctt tttgtaactc tgcctgctgt tgcagttcct gttttgtata ttcccgcttt
     3841 actactttac catccagaaa cttccagttt cccgagatat cggcgcgacg gttggctgtt
     3901 atattcggta actcaacaac acttaacccg tcagggttaa gtgttgatac gtctttctca
     3961 atacaacgaa taatcccctt ctggtcataa gctattttta aagtatcatc ctgaaagttt
     4021 tttaattcgt cataccagtt tttaccgtct tctgtatata gccagataac gccagctttt
     4081 ttcgtaagct gatactgctc aatggttttg gggtttccgg cggtaatatt tttcagatgt
     4141 tgcatatatt ctcctgatgg ctatgcctgt tcaacgttat accagttacc agaaatgtat
     4201 ttttgtagtg gtcgtgacac catccagtca tctccatcaa cctcaccaat aatttgcaat
     4261 cctgttgtca catgaccatt acctggttcg caaagtcctc cgcgatataa tgtgtcagaa
     4321 cgtctgcctc ccagacgcac atttctgata aaagaactat tcaggtaatc atttaag
//
LOCUS       DAOFSV010000248         4342 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.248, whole genome shotgun sequence.
ACCESSION   DAOFSV010000248 DAOFSV010000000
VERSION     DAOFSV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.248"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            109..777
                     /locus_tag="QBH27_004621"
     CDS             109..777
                     /locus_tag="QBH27_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016837.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(1020..1715)
                     /locus_tag="QBH27_004622"
     CDS             complement(1020..1715)
                     /locus_tag="QBH27_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDQ7016838.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAIVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1708..3135)
                     /locus_tag="QBH27_004623"
     CDS             complement(1708..3135)
                     /locus_tag="QBH27_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDQ7016839.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(3146..3865)
                     /locus_tag="QBH27_004624"
     CDS             complement(3146..3865)
                     /locus_tag="QBH27_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDQ7016840.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1179 a         1100 c          887 g         1176 t
ORIGIN      
        1 atccagtact catgatgctg gctttttgct gatatttacc ctgaacattc ctgatacaga
       61 ctattaatac aaccttatct gcgacagatt tcattttctg gatatattat gaatgaacaa
      121 atcaaacagg atatcgatct gattgagatt ttattttatc tgaagaaaaa gattcgtgtt
      181 atccttttta ttatagctat ctgtatggca atggtgctgt tgtttctgta tatcaataaa
      241 gataatataa aagtgagtta cagcctaaaa ataaatcaga caacaccagg catacttgtt
      301 agctgtgata gcaataataa ctttgcctgt cagaccacaa tgactgaaga tgttattcag
      361 cgaattacta cattttttca gaccagccca gatgtcaaga acagagaaat aaagctggaa
      421 tggtcaggag ataagagaga tttaccaact gctgaagcgg aaatatctcg cgtgcaggcg
      481 tctatcatta aatggtatac gtcagaatac cataatggca ggcaagttct cgatgagata
      541 caaacgcctt cagcaattaa cagtgagctt tatacaaaaa tgatatatct gaccaggaac
      601 tggtcactgt atccgaacgg tgatggctgt gtcactataa gctcaccaga aataaaaaat
      661 aaataccctg ctgccatttg cctggctctg ggattttttc taagtattgt gatttctgta
      721 atgttttgcc ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa
      781 acagcattct gacagagagt gcctgtataa aactatagtg acagtgaccg ggaagggagc
      841 ccgtcttcag gaatggattc ctgtcttttt aactgtaaat atcctggcat tattaatgtc
      901 aggatattta cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtcctt
      961 aacgctattc acaacctcgt ataccgcctt caggtttatg tccagactac atatctctct
     1021 cagcaatcct caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg
     1081 ataagctcaa tgtccgccgt tgaactggga ccactgatga tgttaataca tgacggcatt
     1141 cgttcaccgg cctgcgcttt ctgatgcaat ttttctgcaa gttgcgctac acgcggcagg
     1201 attgtgcttt tacgcaggat aaaaagagaa gattccggga gcaggctcaa tgaacgcccg
     1261 cgcccggcgg cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatat
     1321 acgacgccca ctatagcctg ctctgcttgt gatatgttct caataccttt cgccggatcc
     1381 caaacaacgg cattgcattc ctgctgcaaa cgttcgctaa tccccaattc ctccagcctc
     1441 gtgtcaccgc taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct
     1501 tctgccgcct tcgcctcgct ggtcagttca cagcgcgtca acataacatc gctggcaaac
     1561 tggataaacg cgtcacagcg ctgttgttgg gtaagttggg taagccgctc gttagcatag
     1621 ttgttaagcg gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga
     1681 gcaacgttat tcaaaaattc gctccgatta tccattctta ttctcctgcg cctgatgttt
     1741 cttaaaccaa ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca
     1801 gtcgctaatc gcgccaaatt tgagtggtgt tttgccgcca ttgataaacc agcttgccgc
     1861 atgagcacca gccatcatcc caactttcca caatcctgga tgactattgg cataagcgaa
     1921 catttttatc gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg
     1981 atgacgcaaa atcagttttg acagcggaat acgcaccgga cacacgctgt cacaagcggt
     2041 gcataaagag caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtaacgggga
     2101 aatcaccgca ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg
     2161 atatgccgga caagtattca tacaagcccc gcaacgaata cagcgcagca catcccgaaa
     2221 ttcagaggcc agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg
     2281 accatcaacg tgccccgctt cgcgtggtcc tgtcagccag gtgttgtatc ccgtcaaacg
     2341 tgcaccaacg gcactgcgcg ccagcatggt gatcaataca tctacctctg caaacgtggg
     2401 agcaatacgc tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg
     2461 cgcattacct tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca
     2521 gccggtaata cctatttcag cactgaggaa atcttcgcgg attttttgcc gaatgaataa
     2581 ggtcatcgct tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg
     2641 gatctgatgg cgatctttat gaattgccgg gaccacaaca tgagagggcg gatcttgatc
     2701 cagctggaga atatattcac ccagatcggt ttcaatcacc tgaatcccgg catcctgcaa
     2761 cacatgattg acaccaatct cttcggtcac catcgattta gatttcacta ccttccgggc
     2821 atttttgcgt tgggcaacct gtaaaatgta gcgggtggcg tcttctttgg tttttgcaaa
     2881 atagacgtga ccgccgtttt gtgtcacttt ttctgagagc tggtacagat aagcgtcgag
     2941 attactcaga acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc
     3001 caattcatcg accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc
     3061 tttgcgcatg atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact
     3121 ggttttgatc gacatcttta tatcctcagc ggctcatcag cacttcagca atatgcatca
     3181 ctttgacttt ctgcccttcc cgttgtaatc gtccactgat gttcagcagg caactcacgt
     3241 cagcaccaat taaatactca gggcggactt ccatcaggtg cgcaaccttt tctttcacca
     3301 tctcgccgga tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat
     3361 cctgttcagc aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggtt
     3421 cgtccttcac ccccagctta cgggccaggc tacaagatgg gtgatacacc gctctccctt
     3481 gcaaactggc acctacatcg actaccccta atgtattaac aataaaagag gtgagatcct
     3541 gcatacgcgc ggcaaccttt tcggcacgtg atgcccattc aggttcatcc gccagatacg
     3601 tcgggtaact ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt
     3661 tatcctccag tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccac
     3721 tattgatcgc aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga
     3781 gtttttccag tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac
     3841 aggtgacaaa gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc
     3901 cgatggttcc ctccttccgg gggatcaatc atcaataaat atggcgttct tttttaattg
     3961 gctagcaaca taccacctct catttgtatg acaaatgata atctttcatc taaacttcaa
     4021 atgcaataac taattttatt tctttcggca tcgctcacat tttataggga aatcgacgat
     4081 catttttggc ataagaaatc catttaaaaa ggctacatcc cttataccta ttcagagcta
     4141 cttattcagg ggttattgag atatacactg atggtgtaca aaaaaatcac cgacaataat
     4201 cacgcatcaa gtaatagtcg ggattttatt taattgaagc aatttagttt gcatttttat
     4261 ttaaaaactg agctagagtc catcaaaaaa atatataaaa cccaaaacca ccgttagaaa
     4321 tagaaatagt tgaaaggctc ac
//
LOCUS       DAOFSV010000249         4332 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.249, whole genome shotgun sequence.
ACCESSION   DAOFSV010000249 DAOFSV010000000
VERSION     DAOFSV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.249"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            294..545
                     /gene="yefM"
                     /locus_tag="QBH27_004625"
     CDS             294..545
                     /gene="yefM"
                     /locus_tag="QBH27_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDQ7016841.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKDIIE"
     gene            542..796
                     /gene="yoeB"
                     /locus_tag="QBH27_004626"
     CDS             542..796
                     /gene="yoeB"
                     /locus_tag="QBH27_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDQ7016842.1"
                     /translation="MKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAACRYHY"
     gene            879..1703
                     /locus_tag="QBH27_004627"
     CDS             879..1703
                     /locus_tag="QBH27_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ7016843.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1749..2678
                     /locus_tag="QBH27_004628"
     CDS             1749..2678
                     /locus_tag="QBH27_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7016844.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            2893..2955
                     /gene="yoeI"
                     /locus_tag="QBH27_004629"
     CDS             2893..2955
                     /gene="yoeI"
                     /locus_tag="QBH27_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDQ7016845.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            2945..4303
                     /gene="plaP"
                     /locus_tag="QBH27_004630"
     CDS             2945..4303
                     /gene="plaP"
                     /locus_tag="QBH27_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDQ7016846.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT         1057 a         1005 c         1119 g         1151 t
ORIGIN      
        1 acgcgtgtca taaaagtctc tgtgaatgtt tattcaactg atgtctttta acctaaacca
       61 ctttcacgtt agaaagcaag ggctttttta tatattttat ggcgatagat ttgtttaatg
      121 aatgagcggg gaaagaaggg ggctaagagg actgtcctct aacttgacag caccaaaaag
      181 agcaaagtct gatttttcgt ctcgctggca atgtcttgat ctacaaacta attaataaat
      241 agttaattaa cacccatcat tgtacaatga actgtacaaa agaggagatt tacatgcgta
      301 caattagcta cagcgaagcg cgtcagaatt tgtcggcaac aatgatgaaa gccgttgaag
      361 atcatgcccc gatcctcatt actcgtcaga atggagaggc ttgtgttctg atgtcactcg
      421 aagaatacaa ctcgctggaa gagacggctt atctactgcg ttcccccgct aacgcccgga
      481 gattgatgga ctcaatcgat agcctgaaat cagacaaagg aacggaaaag gacattattg
      541 agtgaaacta atctggtctg aggaatcatg ggatgattat ctgtactggc aggaagcaga
      601 taagcgaatt gttaaaaaga tcaatgaact tatcaaagat acccgcagaa caccatttga
      661 aggtaagggg aaaccagaac ccctgaaaca taacttgtca ggtttctggt cccgacgcat
      721 tacagaggag caccttctgg tatacgcggt tactgatgat tcactgctca ttgcagcatg
      781 tcgttatcat tattgaagag attcattcat agaatgaatt gtcagttttg atacgctagc
      841 gtagccttat agatctaagg tgtatcagga gataacggat gaaaaaggtc gcaattgtcg
      901 ggttagggtg gttaggtatg ccgctggcga tgtcactttc agcgcgaggc tggcaagtca
      961 ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg aatgagtggc attgatagct
     1021 atctgcttcg catggagcct gagttagttt gcgattctga cgatctggat gcccttatgg
     1081 atgccgatgc gctggtcatt acgcttccgg cacgtcgtag cggccccggc gatgagttct
     1141 atttacaagc ggtacaagag ttagtggata gcgcgctggc tcatcgtatt ccccgtatta
     1201 tttttaccag ctcgacgtct gtctatggcg acgcacaagg cacggtgaaa gaaaccaccc
     1261 cgcgtaatcc ggtaaccaac agtggacgag tattagaaga actcgaagac tggctgcaca
     1321 atttacccgg tacttcggtc gatattctgc gtcttgcggg cctggtcgga ccgggacgtc
     1381 atcccggacg cttctttgcc ggaaaaaccg cccctgatgg tgaacatggt gttaatttag
     1441 tccatttaga agatgttatt ggcgctatca ctctgttgtt acaggcacct aaaggcggac
     1501 acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa tgttttctat ccgcagatgg
     1561 cccgtttact ggggctggaa cctccgcagt tcagaaatag tctggacagc ggcaaaggta
     1621 agattattga tggcagtcgg atttgtaatg aactgggatt tgaataccag tatcccgatc
     1681 cgctggtaat gccgctggag taaacgatca cgccagcagg acgccgcatg cactgcaagg
     1741 gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct cgatgcctta gaaaaagaag
     1801 gcagttttgc ggcggcgtca gccaaactct acaagacgcc gtccgctctg agttacaccg
     1861 ttcacaagct ggaaagcgac cttaatattc aattgcttga tcgtagcgga caccgcgcta
     1921 aattcacccg taccggaaaa atgttattag agaaagggcg ggaagttctg cataccgtgc
     1981 gagaactgga aaagcaggcg atcaaactgc atgaaggctg ggaaaacgag ctggtgattg
     2041 gcgtggacga cacgtttcct ttttctctgc ttgcgcctct cattgaagct ttttatcaac
     2101 atcatagcgt gacgcgcctg aagtttatca atggcgtact cggtggttcc tgggatgccc
     2161 tgactcaggg gcgagcggat attatcgttg gcgcgatgca tgagccacca tcttccagtg
     2221 agtttggttt ctcgcggttg ggcgatcttg aacaagtctt tgcggtcgcg cctcatcatc
     2281 cgttagccca ggaagaagaa ccgttaaacc gtcgtatcat taaacgatat cgggcgattg
     2341 tggtggggga cactgcgcag gcaggcgctt ctacggcctc acaacttctc gacgaacaag
     2401 aagccattac cgttttcgat tttaaaacca agctagagct gcaaattagc ggccttggct
     2461 gcggctattt accacggtat ctggcgcaac gttttctcga tagtggcgcg ttaatcgaga
     2521 agaaagtggt cgcccaaact ctctttgaac ccgtctggat tggctggaat gaacagaccg
     2581 cagggcttgc cagcggctgg tggcgagatg aaattttagc aaatagtgcg atcgccggtg
     2641 tttatgcaaa atctgatgac ggaaaatcag ccatttaaag aaaaattatt atggcaagcc
     2701 tctcattctc ttgtcatttc ccccccattt aggcacaatg cgccgctgtc aaaaaatgac
     2761 taaaaaccga cgtttaatta gcgtcggtta ttttttgctt caaaccaatc attcatacca
     2821 agaggccggg cttcgtaccg gatagatatt tactaaaaat cgacagttgt tgtcgctgag
     2881 gaatccaaaa aaatggggca attttttgct tacgcgacgg ttatcaccgt aaaggagaat
     2941 gaccatgtcg cataacgtta ctccaaacac ctctcgcgtg gaattgcgta aaacgcttac
     3001 gttagttccg gttgtaatga tgggtctagc ctatatgcag ccgatgacgc tgtttgatac
     3061 atttggtatc gtttcaggcc tcacggatgg tcatgtgccg acagcctatg cgttcgcatt
     3121 gattgcgatc ctgtttacgg ctctgagcta cgggaagctg gttcgccgct atccttctgc
     3181 tggctctgca tacacttacg cccagaaatc cattagcccg actgtcggct ttatggtggg
     3241 ttggtcttct ctgctcgact atctgttcgc gccgatgatc aacattctgc tggcgaaaat
     3301 ctattttgaa gctctggtgc cttccattcc atcgtggatg tttgtggtgg ctctggtggc
     3361 ctttatgacc gcctttaacc tgcgtagtct gaaatccgta gcgaacttca acactgtaat
     3421 cgtcgtgttg cagatagtgc tgatcgcggt gattctgggc atggttgttt atggcgtatt
     3481 tgaaggtgaa ggcgctggta cgctggcgag cactcgtccg ttctggtctg gcgatgcgca
     3541 tgttatcccg atgattaccg gggcgacaat cctgtgcttc tcctttaccg gttttgacgg
     3601 catcagcaac ctgtcggaag aaaccaaaga tgcagagcgc gtgatcccgc gtgcgatttt
     3661 cctgaccgcg ctgatcggcg gcatgatctt catctttgca acttacttcc tgcagctgta
     3721 cttcccggat atctctcgtt ttaaagatcc ggatgcgtca cagcctgaaa tcatgctgta
     3781 cgttgcgggt aaagcgttcc aggttggcgc gctgatcttc tccaccatta ccgtactggc
     3841 gtccggtatg gcggcgcacg caggcgttgc gcgtctgatg tacgtaatgg gtcgtgacgg
     3901 cgtgttcccg aaaagcttct tcggttatgt acacccgaaa tggcgtactc cggcgatgaa
     3961 tatcatcctg gttggcgcga ttgccctgct ggcaatcaac ttcgacctgg taatggctac
     4021 ggcgctgatt aactttggtg cgttggtggc gttcaccttc gttaacctgt cggtcatctc
     4081 gcagttctgg atccgtgaga agcgtaacaa aacgctgaaa gatcacttcc agtatctgtt
     4141 cctgccgatg tgtggtgcgc tgaccgttgg tgcgctgtgg gttaacctgg aagaaagctc
     4201 aatggttctg ggtctgatct gggctgctat cggtctgatt tacctggctt gcgtaaccaa
     4261 gagtttccgt aacccggttc cgcagtacga agacgtagcg taatcttaca aaccggatga
     4321 cataatcatc cg
//
LOCUS       DAOFSV010000250         4282 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.250, whole genome shotgun sequence.
ACCESSION   DAOFSV010000250 DAOFSV010000000
VERSION     DAOFSV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.250"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            1212..2780
                     /gene="cydA"
                     /locus_tag="QBH27_004631"
     CDS             1212..2780
                     /gene="cydA"
                     /locus_tag="QBH27_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDQ7016847.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2796..3935
                     /gene="cydB"
                     /locus_tag="QBH27_004632"
     CDS             2796..3935
                     /gene="cydB"
                     /locus_tag="QBH27_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDQ7016848.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3950..4063
                     /gene="cydX"
                     /locus_tag="QBH27_004633"
     CDS             3950..4063
                     /gene="cydX"
                     /locus_tag="QBH27_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDQ7016849.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4063..>4282
                     /gene="ybgE"
                     /locus_tag="QBH27_004634"
     CDS             4063..>4282
                     /gene="ybgE"
                     /locus_tag="QBH27_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDQ7016850.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKV"
BASE COUNT          986 a          972 c         1056 g         1268 t
ORIGIN      
        1 taatgataat aagacatatt cttatgctgc ttaattacga tctgttttta tatgattagg
       61 tgtcaatacg ttatatcact cgatgtgtta ctgacgacta ttactttaat atttagtgat
      121 catagacacg gttctccaga ttactaaatt tctggcatct gacaatatca tcatcttcaa
      181 atagttaaat taagattatt gcacttccaa aacagataaa ttgaagcagt ctggtagata
      241 ttgcagaaca accttgattc caacggcgaa atcatgttta tcctccaggt tgccgcccat
      301 atggagctga atatcattca gtccacgctt gatgagtcga tatcgctata tccatagaca
      361 gggtagcttt ttacacgaga gtgtccggtg ttttctaacg gctggcaatc attccgtttt
      421 gttgtctcgt atcactgcta taacaatttc tcttgatttt caatctttga gcgaatcatt
      481 gaatacagat cgggaaatat taataccata taaatttatt ttgaaaatcc ttgttccatc
      541 tgactatacc taaggtatct atttcttctt gtgattatta ccaatgaaac aataagtgac
      601 ggatttacag tacgttgctc ataaagacaa caatcaatta acgttgataa aaatatgtta
      661 tgtaaaatgg caacaaagta gctactctaa ctaactagta ttataaaaag tcgataaggc
      721 ggttctgtta aataaaatac tggtttttta tctatatatt tccatgtggg tagttttact
      781 gtgttatttt gctacaattt attaacaaca aacccatcaa tgattcatga tttacgagcg
      841 gttaacatat ctgattgatt ttgatataaa tcaattttta attcttgaaa aaaatctgca
      901 aaacacgata aattgttgta gatcaaattg gtgggatcgt gacaatttcg ctataaattg
      961 atcactgtcg aaaaatgcaa atttgctcaa taaaaacctg tttattgtaa ggattttgca
     1021 gcgtaatata attacgggat aaatttggtt tattcattaa catatttgta acttttttat
     1081 cattgtcttt tcttcttttg atgaaatggc aacgatagag gcgagcaaat aattttgtat
     1141 tggggcatgc gtgtgaccct ttctaacggg gttcactctc ggagtcttca tgcgatgagc
     1201 aaggagtcat gatgttagat atagtcgaac tgtcgcgctt acagtttgcc ttgaccgcga
     1261 tgtaccactt cctttttgtg ccactgacgc tcggtatggc gttcctgctg gccattatgg
     1321 aaacggtcta cgtcctttcc ggcaaacaga tttataaaga tatgaccaag ttctggggca
     1381 agttgtttgg tatcaacttt gctctgggtg tggctaccgg tctgaccatg gagttccagt
     1441 tcgggactaa ctggtcttac tattcccact atgtagggga tatcttcggt gcgccgctgg
     1501 caatcgaagg tctgatggcc ttcttcctcg aatccacctt tgtaggtctg ttcttcttcg
     1561 gttgggatcg tctgggtaaa gttcagcata tgtgtgtcac ctggctggtg gcgctcggtt
     1621 caaacctgtc cgcactgtgg attctggttg caaacggctg gatgcaaaac ccaatcgcgt
     1681 ccgatttcaa ctttgaaact atgcgtatgg agatggtgag cttctccgag ctggtgctta
     1741 acccggttgc tcaggtgaaa ttcgttcaca ccgtagcttc tggttatgtg actggcgcga
     1801 tgttcatcct cggtatcagc gcatggtata tgctgaaagg tcgtgacttc gccttcgcta
     1861 aacgctcctt tgctatcgct gccagcttcg gtatggctgc cgttctgtct gttattgttc
     1921 tgggtgatga atccggctac gaaatgggcg acgtgcagaa aaccaaactg gctgctattg
     1981 aagccgagtg ggaaacgcaa cctgcacctg ctgcctttac tctgttcggc attcctgatc
     2041 aggaagagga gacgaacaaa tttgcgatcc agatccctta cgcgctgggc atcattgcaa
     2101 cgcgttccgt ggatactcca gttatcggtc tgaaagaact gatggtgcag catgaagaac
     2161 gcattcgtaa cgggatgaag gcgtactctc tgctcgaaca actgcgttct ggttctactg
     2221 accaggccgt tcgtgaccag ttcaatagca tgaagaaaga tctcggttac ggtctgctgc
     2281 tgaaacgcta tacgccaaac gtggctgatg cgactgaagc tcagattcaa caggcaacca
     2341 aagactccat tccgcgtgta gcgccgctgt acttcgcgtt ccgtatcatg gtggcgtgtg
     2401 gcttcctgct gctggcaatc attgcgctct ctttctggag tgtcatccgc aaccgcattg
     2461 gcgagaaaaa atggctgctg cgcgccgcgc tgtacggtat tccgctgccg tggattgctg
     2521 tagaagcggg ctggttcgtg gctgaatatg gccgccaacc gtgggctatc ggtgaagtgc
     2581 tgccgacagc tgtggcgaac tcgtcactga ccgcaggcga tctcatcttc tcaatggtgc
     2641 tgatttgcgg cctgtatacc ctgttcctgg tggcagaatt gttcttaatg ttcaagtttg
     2701 cacgcctcgg cccaagcagc ctgaaaaccg gtcgctatca ctttgagcag tcttccacga
     2761 ctactcagcc ggcacgctaa gacaggagtc gtcaaatgat cgattatgaa gtattgcgtt
     2821 ttatctggtg gctgctggtt ggcgttctgc tgattggttt tgcagtcact gacggtttcg
     2881 acatgggggt gggcatgctc acccgtttcc tcggtcgtaa cgacaccgag cgtcgaatta
     2941 tgattaactc catcgcacca cactgggacg gtaaccaggt ttggctgatc accgcaggcg
     3001 gtgcactgtt tgctgcctgg ccgatggtct atgccgctgc gttctccggc ttctatgtgg
     3061 cgatgatcct cgtactggcg tctttgttct tccgtccggt cggttttgac taccgctcca
     3121 agattgaaga aacccgctgg cgtaacatgt gggactgggg catcttcatt ggtagcttcg
     3181 ttccgccgct ggtgattggt gtagcgttcg gtaacctgtt gcagggcgta ccattcaacg
     3241 ttgatgaata tctgcgtctg tactacaccg gtaacttctt ccagctgctt aacccgttcg
     3301 gcctgctggc aggtgtggtg agcgtaggga tgatcattac tcagggcgca acctatctgc
     3361 aaatgcgtac cgtgggcgaa ctgcacctgc gtacccgtgc aacggctcag gtggcggcac
     3421 tggtgacgct ggtctgcttc gcactggctg gcgtatgggt gatgtacggt atcgatggtt
     3481 atgtcgtgaa atcgacaatg gaccattacg cagcctctaa cccgctgaat aaagaagtgg
     3541 ttcgtgaagc tggcgcatgg ctggttaact tcaacaacac gccaattctg tgggctattc
     3601 cggcactggg tgtggttctg ccgctgctga ccatcctgac tgcacgtatg gataaagccg
     3661 cgtgggcgtt tgttttctcc tccctgacgc tggcctgcat catcctgact gccggtatcg
     3721 caatgttccc gtttgtgatg ccgtccagca ccatgatgaa cgcaagtctg acaatgtggg
     3781 atgcaacttc cagccagctg acgcttaacg tcatgacctg ggttgcggtg gttctggtac
     3841 cgatcattct gctctacacc gcctggtgtt actggaaaat gttcggtcgt atcaccaaag
     3901 aagatattga acgtaacacc cactctctgt actaagtaag gagctaaaaa tgtggtattt
     3961 cgcatggatt ctgggaacgc ttcttgcctg ttcgtttggg gtaatcaccg cgctggcgct
     4021 tgagcacgtc gaatcaggca aagccggtca agaagacatc tgatgagtaa gattatcgca
     4081 accttgtatg cggtaatgga caagcgcccc ctgcgggcgc tttccttcgt gatggcactt
     4141 ctgttagcag gatgtatgtt ttgggaccca tcacgtttcg ccgcgaagac cagtgaactg
     4201 gaaatctggc atggtttact gcttatgtgg gccgtctgtg ctggtgtgat tcacggcgtg
     4261 ggctttcgtc cgcagaaggt tc
//
LOCUS       DAOFSV010000251         4194 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.251, whole genome shotgun sequence.
ACCESSION   DAOFSV010000251 DAOFSV010000000
VERSION     DAOFSV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.251"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(3..269)
                     /gene="ariR"
                     /locus_tag="QBH27_004635"
     CDS             complement(3..269)
                     /gene="ariR"
                     /locus_tag="QBH27_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDQ7016851.1"
                     /translation="MLEDTTIHNAISDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(298..570)
                     /locus_tag="QBH27_004636"
     CDS             complement(298..570)
                     /locus_tag="QBH27_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDQ7016852.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKNMFTGKKINRIH"
     gene            complement(613..849)
                     /locus_tag="QBH27_004637"
     CDS             complement(613..849)
                     /locus_tag="QBH27_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDQ7016853.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            1163..2374
                     /gene="bluF"
                     /locus_tag="QBH27_004638"
     CDS             1163..2374
                     /gene="bluF"
                     /locus_tag="QBH27_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDQ7016854.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
     gene            2579..3310
                     /gene="bluR"
                     /locus_tag="QBH27_004639"
     CDS             2579..3310
                     /gene="bluR"
                     /locus_tag="QBH27_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDQ7016855.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTRQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3531..3935
                     /locus_tag="QBH27_004640"
     CDS             3531..3935
                     /locus_tag="QBH27_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDQ7016856.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3988..4113
                     /locus_tag="QBH27_004641"
     CDS             3988..4113
                     /locus_tag="QBH27_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016857.1"
                     /translation="MEIKVQRPSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
BASE COUNT         1240 a          866 c          835 g         1253 t
ORIGIN      
        1 agttacatat catcagctgt gtatcgcaac acgatttcca gtgtttttct aatcacatca
       61 gctttgagaa tatcacttgt tgtttccagg gagtgtatca gacttaagat aatattttta
      121 ttattaacat tatcacctga aagcattaaa ttggtcacag cctgccctaa cacagctgac
      181 tcttcttcta acaaattacc tgaactacga aagtaacttg ataacgcttt atcagatatt
      241 gcattatgaa tagtagtatc ttcaagcatg gtaacctctc atcttactta tgaaatttta
      301 atgtattctg tttattttct taccagtgaa catattttta ttgtcagcga tcgctgttct
      361 tcggtttggc cctcctgtat cactgttgct aaaatcacga attatgctgt caattaaatc
      421 tcgtcggaaa gtattttcct cagccgattt cattacgagt aattgcttaa taagagtctg
      481 caacgaaaaa tttatcttca tgcgcagtat ctggagcacc gcctggccag taatttcata
      541 tttaatactt tcattatcag atgtcttcat aacgacttca tagctaacct gttaaataat
      601 caggctgttg cattattcaa aaagcaaccc aattagtgca ttataatgtt tctgttcctc
      661 ttcccctgtc gcttgttcca gtcggcaaag aagtttggca caaagcgact ttcgactcag
      721 attacgtcca tctttcaaaa tctcagtcac aatttccccg agagtttcct gctgagtagg
      781 caagttagct tttgcaaagt aacgagtgat tgctcctgca gaatctgaag tcactgaatt
      841 ttgatgcatg ctacgcctct gttaaaaatg ttaatcaatg ttaagtgagg taacaatagc
      901 aaacttgtac gaaatatgtg tacagaatga taaattaatt tttcgcaatt agtgctaatt
      961 catataaaac aatgcattac gataaaacat attgcatcat ttaaatgaca acctatacag
     1021 gttttttcac caaaccgtct tcaacctttt ccacttttta tcaatgtaaa gaaacggtta
     1081 tgttttggta ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt actctcgcct
     1141 cgcccattac cctggattgt taatgcttac cacccttatt tatcgtagcc atatacgtga
     1201 cgccgaacct gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgcg
     1261 gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg
     1321 tccggaagaa caggttcaaa tgatatatcg ggccatatgc caggatccac ggcactataa
     1381 tattgttgaa ctgttgtgcg attacgcgcc tgctcgccgt tttggcaaag caggaatgga
     1441 attatttgat ttacgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg
     1501 cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt
     1561 ccttgcaact gaacaatcaa cctatttcga gattcctgct gaagactcct ggctttttat
     1621 cgctgacgga tctgataaag aacttgattc ttgtaccctt tcatcaaata taaacgacca
     1681 ctttgccttt catcctattg tcgacccttt atcgcggcgg ataatcgctt ttgaagccat
     1741 tgtgcaaaaa aatgaagaca gcccgtcagc tatagcggtt gggcagcgta aagaagggga
     1801 aatctacaca gcggatctca aaagtaaggc gcttgcattc gcgatggcac acgcacttaa
     1861 gctcggtgat aaaatgattt caatcaatct attacctatg accctggtta acgagcctga
     1921 cgcagtctct tttttacttg atgaaataaa ggccaatgct ctggtgcctg aacaaatcat
     1981 cgttgaattt actgaaagtg aagtcatatc tcggtttgat gagtttgccg aagcggttaa
     2041 atcgctaaag gctgccggta tcagtgtagc aattgatcat tttggcgcag gttttgctgg
     2101 tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac
     2161 caacgttcat aaaagcggac cacggcaggc aattattcag gcgatcatga aatgctgtac
     2221 atcacttgaa attcaagtct ctgctatggg cgtggcaaca ccagaagagt ggatgtggct
     2281 tgaatctgca ggaattgaga tgtttcaggg agatctgttt tccaaagcta aattgaatgg
     2341 tatcccttca gttgcgtggc cggagaaaaa ataattttca gcacattatt tcgcatgatt
     2401 tctggaaatc atcgtgaaaa tgaatacgtt atgttttaat ttaatccaca ttaaaaataa
     2461 tatttcatcc aatttagctg aaatcttatt tatactttgt acaattttag cgtatattgt
     2521 acaaagtatt attgggtcgt gtagaggcga cggagattta tgaccgcaag gaggaattgt
     2581 ggcttattac agcattggtg atgttgctga acgttgcggg attaatcctg tcactctccg
     2641 ggcctggcaa cgccgctacg gtttgttaaa accacaacgc agtgaaggcg gacaccgact
     2701 ctttgatgaa gaagacatac aacgcatcga agagatcaag cgttggataa gtaatggcgt
     2761 tcctgtaggc aaagttaaag cattactgga aactacccgc caggatacgc aagatgactg
     2821 gagccgcctg caagaagaga tgatgtcaat tcttcgtatg gctaatccag ccaaactacg
     2881 cgcgcgaatt atttcactgg gtcgagagta cccagtagat caattgatta atcatgttta
     2941 ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc tcccgcatta tgagcagtat
     3001 gtttgacggc gcattaattg aatacgcagc ggcctcactt ttcgaaatgc gccgtaagcc
     3061 cggtaaagag gccattctga tggcgtggaa tgttgaagag agagcacgat tgtggctgga
     3121 agcatggcgt ttatcattgt caggatggca catttctgtt cttgctgatc ccattgaatc
     3181 gccgcgcccg gaactgttcc caacgcaaac attgattgtc tggacaggca tggcaccaac
     3241 gagaaggcaa aacgaacttt tacaacactg gggtgagcaa ggttataaag tcatctttca
     3301 tgccccctaa attcaccttt atcccgcccc ttagacagaa tgttataatc tgaacccaca
     3361 acagcgtttg tagcggagct ggtccgttgc aaaatgctca ccgggagaat tcaggtaatc
     3421 cccctttgac actttgtaca tatcacagcc aaaggacaga ccagattgaa gtggtaaaac
     3481 cgatcgtgca cactacgcag tctcacacct aacaagaggt tattatcctg atggatcagg
     3541 tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga gcaaaacttc tcctggtttt
     3601 attcagaatt aaaacatcac cgtattgcac attacattta ttatctggct acggataaca
     3661 tcagaattat tactcacgat gacacggttt tgttattaag aggaaccagg aacctgttaa
     3721 aagttagtac gaccaagaac ccagctaaaa taaaagaggc cgcattgctt catattcgcg
     3781 gaaaatctac atttcgggaa tactgttcaa cactggcagg cgcaggcgtt ttccggtggg
     3841 ttactgatgt taatcataac aaacgcagtt actacgccat tgataatacg cttttatata
     3901 ttgaagatgt agaaaataat aaaccattaa tctagctaaa gttgggagct taagaaatgc
     3961 ttcataattc agtaaggcat tagcataatg gaaataaaag tgcagagacc atccctatgg
     4021 atgattaata ctgtcttttt attgcttccc ataaataatc accagaccaa tacgatcaaa
     4081 ttgatatttg aaatgtgttc acttgacttt tgatacgtta ttttataatg gttaacatat
     4141 ttataacaac aacgggcgtg ttatacgccc gttaccgttt ttaacacatg caga
//
LOCUS       DAOFSV010000252         4121 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.252, whole genome shotgun sequence.
ACCESSION   DAOFSV010000252 DAOFSV010000000
VERSION     DAOFSV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.252"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..575
                     /locus_tag="QBH27_004642"
     CDS             <1..575
                     /locus_tag="QBH27_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7016858.1"
                     /translation="MGASGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRF
                     RAEKIGLVFQQFHLIPFLTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQ
                     LSGGEQQRVCIARALVNEPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTH
                     NPALGQFADRILRLQHGKYLGEEANQHALA"
     gene            562..1047
                     /locus_tag="QBH27_004643"
     CDS             562..1047
                     /locus_tag="QBH27_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDQ7016859.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAAFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPSELEATFARLAA
                     R"
     gene            1072..1557
                     /locus_tag="QBH27_004644"
     CDS             1072..1557
                     /locus_tag="QBH27_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016860.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            1679..2312
                     /locus_tag="QBH27_004645"
                     /pseudo
     CDS             1679..2312
                     /locus_tag="QBH27_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
     gene            complement(2462..2635)
                     /locus_tag="QBH27_004646"
                     /pseudo
     CDS             complement(2462..2635)
                     /locus_tag="QBH27_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2636..2950)
                     /locus_tag="QBH27_004647"
                     /pseudo
     CDS             complement(2636..2950)
                     /locus_tag="QBH27_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3623..4121)
                     /locus_tag="QBH27_004648"
                     /pseudo
     CDS             complement(3623..4121)
                     /locus_tag="QBH27_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(3965..4081)
                     /locus_tag="QBH27_004648"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          925 a         1108 c         1145 g          943 t
ORIGIN      
        1 ttatgggcgc ttctggttct ggtaaaacca cgctgatgaa tattctgacg tgccttgata
       61 cggcgacgga agggcaggtg tttcttgatg gcaccgatgc cgccgcgctg gatgaagagg
      121 ggcgccgtcg ctttcgcgcc gagaaaatcg gcctggtatt tcagcagttc catttgatcc
      181 cgttcttaac tgcgctggaa aacatcatgc tcgcccagca ttaccacagc gtggtggatg
      241 aagcggcggc gaggaaggtg ctggaacagg tagggcttgg gcatcgtgtg acgcacctgc
      301 cgagccagct ttccggtggt gagcagcagc gtgtgtgtat cgcccgggcg ctggtcaacg
      361 aaccgccggt tattttcgcg gatgaaccga cgggcaacct ggatgaagag aatgagcagc
      421 gggtgctgga tctgctcacc gatctgcatc gtcaggggcg cacgattgtg atggtgacgc
      481 acaaccctgc gcttgggcag tttgccgacc gtattctgcg gctgcaacac ggtaaatatc
      541 tgggtgagga ggccaatcaa catgcgctgg cataatgtgc tgttactcgc ggcggcgatc
      601 ctgataagcg ggtgtaaaga agaaaagctg gcggtcgggg caccggcccc ggcgctggcg
      661 gcgtttgatc tgcaaggcca ggagtcaggc cttgaacgct ggcaaggaaa ggcggtgtac
      721 ctgaattttt ggtctgcggg atgcgggggg tgcctagcgg aaatggacac gctggaagcg
      781 ctgagcaaaa aatggggcga taaagtggtg gttgtggcgg tcaataccga ccctgaaacg
      841 gtcaggatca acgatctgct ggcaaaacat cagataacct atccggtggt gcgcgatcag
      901 ctcaacatta cccaggaacg ttatcaggtg atcggcacgc cgacctccgt actgattgac
      961 tccgaaggac gcgttctgga gctacaccag ggtatgcgta agccgtctga gttagaggcc
     1021 acgtttgcgc gcctggcggc gcgatgaacg acgcgtacgg agagataccc aatggcattt
     1081 atctggaacg acgaaagcct tgcgattctg cgggaaaacg cgggcatcct cacgacagag
     1141 cagatagcgc aactgcttca cactaatatt accgccgtgc ggaatatggc ctatcggctg
     1201 aaactgagcc ttcgggtgac ggcgtataat caccgacgca tagcgcaggt tcaggcgctt
     1261 tatgcctctg agacgttgag tctcaaagag atcgcggcga aaacggggct gacggccagt
     1321 accgtgcaat atatcgtgta cgtgaaatca aaaaataagc cctacgccac cacggaatat
     1381 gtctcttttg aaacggagaa cgccgttcat taccgcgtgc aaaaagaatt tgtcgatacg
     1441 gaacgttccc tgctggacaa catctctgat aacacgcgat ttcgtgaact gtatctgacg
     1501 gacggcactt tttattgcgc ccgcaatatt aagtatgagg tttttatctc ggagtaaccg
     1561 gaacacgctg acctgccggt tatcttctgg aatctcccgt tttgcgaaac cgttcccgga
     1621 aaaatgtcat atttctgtca cactctatga ggattgataa caacaagagg ttgtagggat
     1681 gaaaaaattg atactcgccg ccgctgtggc aggggctgtt ttattgtcgt ccgccgcgca
     1741 agcgcagaca acgccggaag gttatcaact gcaacaagtg ttgatgatga gccgacacaa
     1801 cttgcgcgcg ccgttggcca ataatggcag cgtgctggag cagtccacgc cgaatcagtg
     1861 gccggagtgg gatgtgcccg gcgggcagct caccacgaaa ggcggtgtgc tggaaattta
     1921 tatgggccac tacatgcgag aatggctggc ggagctgggg atggtcacgt caggcgaatg
     1981 cccgacgccg gacacggtat atacctatgc caacagtctg caacgcaccg ttgcgacggc
     2041 acaattcttt atcaccggcg ctttccctgg gtgcgacatt ccggtacatc atcaggaaaa
     2101 aaatgggcac gatggaccca acgtttaatc cggtgatcac cgatgactct gcggcgttta
     2161 gccagaaagc ggtgcaggcg atggaaaaag agcgcagcca gatgcagctt gacgacagct
     2221 atcagctgct ggcgcagatg acggactaca aagattcacc gtcctgcaaa gaaaaacagc
     2281 agtgttcgct gaccgaagcc aaagacgcct ttagggaagg tgcgaataag caggtcatgt
     2341 cttcccaagc tgactcgttg attaaaattt cgcggatctg ggccgatttt tgtcccgcaa
     2401 acacatcgaa tcagcctatt taggctattt tttccaccat ttctggtgtt atttccggtt
     2461 tttactgaga tctctcccac tgacgtatca tttggtccac ccgaaacagg ttggccaggg
     2521 tgaataacat cgccagttgg ttatcgtttt tcagcagccc cttgtatctg gctttcacga
     2581 agccgaactg ccgcttgatg atgcgaaacg ggtgctccac cttggcacgg atgcttatac
     2641 gatcccgcaa cgcggggata cccagtggtc gcagtttgcc gttgctttta gggatgtaaa
     2701 cccgtctggc gggcaagggc tggtagtggc ctgagagtaa ttcatccctg aggatttgca
     2761 gctcaacagc cagtctggcc tgtagcattg ttttgttcac gccatcaacg ccgggggtat
     2821 gggccccctt tgatgaaagc gtgatccgcg ccgcttcagc cagccattct ggttgtgtta
     2881 tcagacgcag cagccgttga atccgtaggg acggatcggt ggctgcccat gtggcaagct
     2941 tgcgttgcat ttcgctgatt atcaaaggtc ttcacctcgt taggtcagtt aattcacgtc
     3001 gcaaacacat tcaaactgct tcccttcgcc atgtaatggg ctttccccat cgcggactac
     3061 tacggaagct ccgccagcca gcgcgtcatc ggagccatgc ccccttaaca tccgtcgctg
     3121 accttccccg gtttacctgc ctggactcag gcatactgag gaggctgccc gtcgcactct
     3181 ttatccttgc ttgccgcaag ttggcagaag tcagcaacgc aagcgtgata gacgctgctg
     3241 ccccggtgtt tcgcatacat gtcaaaacac cttcgaccgg tagtgcttac gtatcactgc
     3301 cagttcctcc tgcacggcct gtcagatcac gtaggccgtg gtgacgtttt caacccacag
     3361 aggcggatta acgggttcat gttcttcagc ctttcagtac ttaaccttga ggatcatctc
     3421 ggcttagtga tctcgcctca atccccgttg tcagcgggtt acatcaccct gcgggcatgc
     3481 cgcaggtcac tgccgctcag gttctccacc gtcacacccg gtgggattgt tgggtttctc
     3541 atcgtgagtt accggttcaa tattccagac agactcgcgg ttcatttaag catccatgcc
     3601 cgccctgaac tccgggcaca ccgtaagtaa aatcagccac ccacaactgg tcagaacgtt
     3661 ctgccacaaa ctgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag
     3721 tgcggacctt tttaccccgg agaacaccgg caagtcccat aaccgccatg aggcgcgcca
     3781 ctgtacatct ggctaccctg attccttccc gtaacaactg acgccagact ttacgcacac
     3841 cgtatacctg atgattccca tcgtatacgc gcagtatctc tttcttcagc cagtcatcgc
     3901 gctgcgcacg ggcactgcgt ttatcaggat gatgtcgctg ttgctgacag tggtaatacg
     3961 ttgacggggc aatatgtagt tcactgcata ccggtccgac cccgtacagc ttacgcagct
     4021 tatccagcag tggcatcact ttttccagag gcggtcgaac tccgccttcg caaaataagc
     4081 ggaagcctgg cgaaggatat cgttactgcg gcgcagttca c
//
LOCUS       DAOFSV010000253         4106 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.253, whole genome shotgun sequence.
ACCESSION   DAOFSV010000253 DAOFSV010000000
VERSION     DAOFSV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.253"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="QBH27_004649"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="QBH27_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ7016861.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="QBH27_004650"
     tRNA            complement(249..324)
                     /locus_tag="QBH27_004650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="QBH27_004651"
     tRNA            complement(331..405)
                     /locus_tag="QBH27_004651"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="QBH27_004652"
     tRNA            complement(522..606)
                     /locus_tag="QBH27_004652"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="QBH27_004653"
     tRNA            complement(615..690)
                     /locus_tag="QBH27_004653"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="QBH27_004654"
                     /gene_synonym="panK"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="QBH27_004654"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDQ7016862.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="QBH27_004655"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="QBH27_004655"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDQ7016863.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGESLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNSLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="QBH27_004656"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="QBH27_004656"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDQ7016864.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKVLLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGRAAVHRQQALVLINEDNAKSEDVVQLALHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1076 a         1014 c          906 g         1110 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgtgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg gagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctctgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttttgg aacagtttct tggtaccaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggtagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttaca gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 tattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgcgagcgtg ccagtttgat cctgacgaaa agtgctaatc atgcagtaga
     1981 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagcac cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccaactttt cccagcgcga aagataaggt
     2221 gccaatcctt cttgctcgaa gagttccagc gccgcgcgta attcacgtat cagcatggcc
     2281 gcaagactat tacgatcgag attgatcccg gcttcctgca gcgtgatcca cccttgatta
     2341 acgacactct cttcaacacg acgcattgcc atgttgatcc ctgcgccaat gactatttgc
     2401 gccgcatcgc cggttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttaacacga actttatctg cacccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgaccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa cgggcagcac agttacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatagg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattcct aacgattcac ccaactgctc accagagtga
     2941 aattcgccat tcgctaacag cgcgattaat ttcagcggaa cggtattatc cttcatgaaa
     3001 tcgtctccac tgcgctaact tcacctgatg caccaataaa gcgaacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatgaag tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagga ctaacgcctg ttgacggtgc accgcagccc
     3181 tacccatttg catccctttt agctggcact gatcgataag ccaacctgct gccagtttta
     3241 ctgaaccatc cgcctgggga taatttggtg ctgttggaaa ttgtgccagt aatactttag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgtcag tacaggttgc cactcttttg
     3481 gcagacgcag gcctacggct acaatagcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttacaaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttat
//
LOCUS       DAOFSV010000254         4099 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.254, whole genome shotgun sequence.
ACCESSION   DAOFSV010000254 DAOFSV010000000
VERSION     DAOFSV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.254"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(138..2675)
                     /locus_tag="QBH27_004657"
     CDS             complement(138..2675)
                     /locus_tag="QBH27_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ7016865.1"
                     /translation="MKGSLKKSLLLLRITIICPVFSCAAVSPSPDNSHRFSFNPDFLQ
                     LSDGDNAKNIDLNYFANASGAAPGEYTVDVVMNGELVDRQIKIDFFAQDNKLNARLTP
                     QQLERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQKLILNIPQSYLKPQDWL
                     STPPHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSSLNLGGWRLRHNGNWST
                     NSYNKESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDSFPFEGAQFSSDDGMIA
                     PELSQYSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELRDFNQIFTGDLEVEIRE
                     ADGTIRHFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNSVSEPQFVQSSAAIGLP
                     YEYTFYGGGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQFSQNYKSFGKQQGQSY
                     RFMYSRGFGETNTTLNITGYRYATRGYYDFDELQQIQSGFVDEKNINSYHQRSRISTT
                     ISQDLQDWGQLYLSASKDQYWDTADGYNLSASYSLPFRYISAMLSLGYNKSPYYHEAD
                     KSLFLSVSVPLNSLLNGNNLFLTTNTITNNGQVQQQVGLNGSSQDGEFNYAVAQGWQN
                     QNRGESGNINLNYRGPYAQMNGGYAWQKESSQWTYGISGGITLHPHGLTLSQPLSLDS
                     ASALVQARDAASVKVLNGSGIYTDWRGYAVVPYLNPYNRNQISLDVNSVKDNVELLNT
                     DVTVIPSRGALVSAPFKVNVGNKAIITLIQKNGEPVPFGSVVTLDSENSINSSIVADQ
                     GQVYMSGLPEEDTLIAQWGEEASQKCKTNYKLQNKKAKFSELTLQCQ"
     gene            complement(2708..3400)
                     /locus_tag="QBH27_004658"
     CDS             complement(2708..3400)
                     /locus_tag="QBH27_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ7016866.1"
                     /translation="MKKYNITILSFIFFSHISLAETGGIYLDTTRVIFDSNDLSTKVK
                     FNNNTDKNWLLRAWVSDYNSKNKNDNFIITPPLYKISANESFQFKIDKLKENFPNDRE
                     SIFHINVMAIPPITNDEKDSNNAVQFAINTRIKLIYRPHKINNKNQVSDAYKNLKITS
                     SKETLTVSNPSPYYITMNDVKINGKNITSIKDFMVSPFSTLIIPEKNAKTISYSTIND
                     YGGETPIQNIKL"
     gene            complement(3465..3989)
                     /locus_tag="QBH27_004659"
     CDS             complement(3465..3989)
                     /locus_tag="QBH27_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDQ7016867.1"
                     /translation="MFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCSVT
                     LPNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNGYV
                     YPALNEATGNQQKDFALWMKVGDKGVSSYNAPIELANGTYPIELQMIKISNNYNKNHT
                     GAWSAQYTLTATYN"
BASE COUNT         1194 a          800 c          810 g         1295 t
ORIGIN      
        1 acgggagtat gatgatattc gcagttgaaa agatgatttg tgtccgccgg aagtaataca
       61 aatctggaat gatgttgtta atatgttaga tgaaaataac cccgcatatg cagggttatt
      121 aatctaagaa ctaccgacta ttggcattgt aaagttaatt cactgaattt cgctttttta
      181 ttttgcaatt tgtaatttgt tttacacttt tgagaagcct cctcacccca ttgtgcaata
      241 agtgtatcct cttcaggtaa ccctgacata tacacctgtc cttgatcagc aacaatactg
      301 ctgttaatgc tattttcaga atctaatgtc accacactac caaacggtac gggctcacca
      361 ttcttctgta tcaaggtaat tattgcctta ttaccgacat tgactttaaa tggagcggaa
      421 actaaagctc ctcgagaagg gatgacagta acatctgtat ttagcagctc aacattatcc
      481 ttaacactat taacatcaag tgaaatttga tttcggttat agggattcaa atatgggacc
      541 acggcatatc cacgccagtc cgtataaata cctgacccgt tcaatacttt tacgctggcg
      601 gcatcacgag cctgaaccag agcactggca ctgtccagac ttaatggctg ggataaggtt
      661 aatccatgtg gatgcaacgt aataccaccg ctaattccgt atgtccattg actggattct
      721 ttttgccagg catagccgcc gttcatttgt gcataaggtc cacgatagtt aaggttaatg
      781 ttacctgact ctcctctgtt ttgattctgc cagccttgcg ccacggcata gttaaactca
      841 ccatcctgtg aggagccgtt taatccaacc tgctgttgca cctgaccatt attcgtaatg
      901 gtattcgtgg ttaagaacag gttatttcca ttcagcaatg agttgagcgg tacagacaca
      961 gataaaaaca gcgatttatc agcctcgtgg taatacgggc ttttattata cccgagcgat
     1021 agcattgccg agatatagcg gaatggcagc gagtaactcg cagataagtt atatccatca
     1081 gcggtatccc agtactgatc tttacttgct gagaggtaga gttgtcccca atcctgaaga
     1141 tcttgactta tcgttgttga tatacgcgaa cgttgatgat aactgtttat atttttctca
     1201 tcaacgaatc cactctgaat ttgttgtaac tcatcaaaat cgtagtaacc gcgtgtggca
     1261 taacgatagc cggtaatatt caatgtcgta ttcgtctcac caaaaccacg cgaatacata
     1321 aagcggtaag actgtccttg ctgttttccg aaagacttat aattctgcga aaattgtgag
     1381 cgagcatgag tgacatccag ggagaacgct ccaaaaaaat ctgagtattt acccaggcca
     1441 agcagaactg ctgcatagtt gtctgctttg ataccaccac cataaaatgt atattcataa
     1501 ggtaatccga ttgctgctga agactgaaca aactgaggct cagacacact attattcaaa
     1561 gtagaacttg agcgatattt cccgaatgcc aggttatagc gtaagcgccc ctgacgttgc
     1621 agaatcggta aaacggccgt cgcctgagta aaatggcgaa tagtgccatc tgcctcacgg
     1681 atttccacct ccaggtcacc ggtgaatatt tggttaaaat cgcgtaattc aaacggtcca
     1741 ggtgggacgt tcttttgata aatgacaact ccgttttgct tgacggatac ctgtgcttga
     1801 gaataagcaa tgccacggac aacaggacta tattggctga gttctggggc aatcatgcca
     1861 tcatcagatg aaaactgtgc tccttcaaaa ggaaaactgt caaatatcgc accatccgtt
     1921 gaggtttgcc ctacagtaaa ttgccctccc tgcaggaaag aataatcatg ctgtagataa
     1981 acactaacgg gttgccagtg gctctcttta ttatatgagt ttgttgacca gttaccgtta
     2041 tggcgaaggc gccatccacc aaggttcaga gaactgtcca gagataaaaa ctgactacgg
     2101 ctaccgtagc cattattatt ttgcttaatt ccgttaaata gatagttaac cattagcgct
     2161 ggcatacctt catcccagag atggggcgga gtacttaacc aatcctgagg tttgaggtag
     2221 ctttgtggta tattcaatat tagcttttgg ttgttcgcat cgaatttctc tgttgcgccc
     2281 tgaataatat atgcaatatt ttgctggtaa gccgatgggg aagatacgga gagcttattt
     2341 gtatcaattc cccaacgttc caactgttga ggcgtcagtc tggcgttcag cttgttgtcc
     2401 tgcgcgaaaa aatctatctt aatctgacgg tcaactaatt ccccattcat aactacatca
     2461 acagtatatt cccctggagc tgcaccgctt gcgtttgcaa aataattgag atcgatattt
     2521 ttggcattat caccatcaga taattgcaaa aaatcggggt taaatgagaa cctatgacta
     2581 ttgtcaggtg agggagatac ggcagcgcaa ctgaataccg gacatatgat ggtgatcctt
     2641 aacaataata aggatttttt taatgaacct ttcaaattgt cccccgataa agatgattaa
     2701 ataaatgtta aagtttaatg ttttgaatcg gcgtttctcc gccgtaatca tttatcgtag
     2761 aataactaat tgttttagca tttttttccg ggatgattaa tgttgaaaat ggtgaaacca
     2821 taaaatcctt tatagatgta atatttttcc cattaatctt tacatcgttc atggtaatat
     2881 agtatgggct agggtttgag acagttaatg tttcttttga agatgttatt tttaagtttt
     2941 tataagcatc agaaacttgg tttttgttgt ttatcttatg tgggcgatat attaatttta
     3001 tccttgtgtt gatggcaaat tgaacagcat tgttactatc cttttcgtca tttgtgattg
     3061 gaggaatagc cataacattg atatggaata tgctctccct gtcgttagga aaattttctt
     3121 ttaatttgtc aatcttaaat tgaaagcttt catttgctga aattttatat aacggcggcg
     3181 ttattatgaa attatcattt ttatttttag aattataatc agacacccat gctcgcaata
     3241 gccagttttt atctgtgtta ttattgaact ttactttagt acttaagtca ttactatcaa
     3301 atataactct ggtagtatca agataaatac caccagtctc agctagcgaa atatgagaaa
     3361 aaaatatgaa agataatatt gtaatattgt attttttcat tttatattgt cacattaaag
     3421 aatgctgaat atacaggcat gagtaatcat gcctgttaaa tatattagtt ataagttgca
     3481 gtcaaagtgt attgcgcact ccatgcacca gtatgatttt tattgtagtt atttgatatt
     3541 ttaatcattt gcaactcgat tggatatgta ccattagcaa gttcgattgg tgcattataa
     3601 gaagatacac ctttatcacc gactttcatc caaagcgcaa aatccttttg ttgattaccg
     3661 gtagcctcgt ttagagcagg ataaacatag ccattagact ggaaattagt agttgctacc
     3721 gttagtgatg caggagtggt acaatcggta acactaatcc cctgatctgc ggttacactt
     3781 tttattttct ggtacgcttt tgcagcattt acctcttgat tcgttactgt gccaaaagat
     3841 gctgttgcat taggcagggt aacactgcaa gtcgcggcat taaaagaacc agtagcggag
     3901 acagaaccac tgcttgtctg ggccattgcg gtaccagaaa taagcagagc cgaaatagct
     3961 gcgatggtga ttttattctt agaaaacatt ttcaacctca atcttgattt tcttaaattt
     4021 ttcctatgtg tatagtgatg ggcaaagaga tgtgagaata aaactttcaa aaattagcaa
     4081 cacatcccaa tgcctcttt
//
LOCUS       DAOFSV010000255         4064 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.255, whole genome shotgun sequence.
ACCESSION   DAOFSV010000255 DAOFSV010000000
VERSION     DAOFSV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.255"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            223..4062
                     /locus_tag="QBH27_004660"
     CDS             223..4062
                     /locus_tag="QBH27_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ7016868.1"
                     /translation="MCSKRNIGDMKITVLNQRYKKHFGYPHILMGDVKVTVSAMIACA
                     VLLHPNKLFASVAGTSLPYQTYRDFAENKGEFRPGAENVPLYDKNGNPVTTLNKAPMI
                     DFSSNDSTGVATLVSPQYVVSVKHNVGYQYVKFGYADDSSYALVDRNNHWRDFHTPRL
                     NKIVTEVTPLDITNAGTAKGTYQNADRFPVFYRVGTGTQYVKDTNGKITYLMGAYSYK
                     TGGIVNTPAISDWSFVTNTTNTPLSTYGTPGDSGSPLFAWDADQNKWVLLAVLNSYAG
                     VNGNTNWYTIIPAGDVKNTMKQDADAPVNTKQGEGDIHWSYDEKTGLGSLTQGSTSWE
                     MHGNLGATWPASLNSGKDLTFQGGGTVVLENTVNQGAGTLTFDDDYIVKPVDTQTWKG
                     GGIIVNGEHLVDWQINGVTGDSLHKLGTGTLKINGTGVNPGSLSVGDGTVILAQRADD
                     NGLSQAFSSVSIVSGRPTLVLNDDKQINPDNIKWGYHGGKLDINGNSLTFHKLNGADD
                     GAILTNSGSMANVNLDFNSPDTTATIANIWHGHFTGNLNINNEVTAGTQNDFAIDGGV
                     NAQGSITQQNGRLFMQGHPVVHAVSSQDVANKLKALGDNSVLTQPVSFTQNDWENRQF
                     SMAELNLQNAEFNLARNASLNTRINAEHSTVTLGSEDLYIDLNDGNGVATKPTLGKSK
                     ATAEDDQSRFNGHVQLQQGSALTINEHFAGGIDSADSATTITSTDTTLNQLSRFTQSS
                     LSLGEGAKLTATAGLLSDGTVSSNAGASLSLLSDQPGTMYSAQSWELSGQDTSLNVGA
                     GGIITGDINANDAASISFGTTDINQSTNYYGNINAPLASVTMKDTAWQVNKQSVAKSL
                     TLNGSTLSFNRFGQGGLTSDTLEATNSSFIINADGKAADTVTVNQALTGGNNTLVVIP
                     TTNSVKQGGDPVSLVTAPKNTQSNIFTLNPVSINAGFHSFTPQLDVLETDVNKQWRLE
                     GFYIQPDKAALRTGKSFMDLGYKNFITEINNLNDRMGDLRHTHGETGAWARLNSGSGS
                     ATDGFTGSYTHLQIGADRKHIIEGGELFTGVTATFTSSNNRGTGWSGRTKSTGIGVYA
                     SAMFDSGLYVDTIGKYVRHDNHYSSSALGMPEQDYGSHSWYLGAEAGWRFSLPDETYI
                     QPQTELIYGTVSENQFAWQFNGGEIYMQRKQMQPLIGRTGIEFGKTFRGKDWEMTALT
                     GINYQYDLFKPTVTAFKDLAGDTYINNGKDSRVVFNVGVNTKIKENTRISLNVERSEF
                     GSYNIDKLINANIRYTF"
BASE COUNT         1234 a          785 c          966 g         1079 t
ORIGIN      
        1 tacaaccatt tgatacttat ttgtacatga cgggattttt gctgaattgt cattccttaa
       61 gtacaaaata gcaattctaa aaaatagaat attttgataa taaacaataa aatatatgcg
      121 tgagggttga ctctgagcaa tgcttggatt tattagtttt aagatctttc gagtcagaag
      181 atatatgaat agatagaata gaaattcaat tgtcggaatg aaatgtgttc aaaaagaaat
      241 ataggtgata tgaaaattac agtccttaat cagcgatata aaaaacattt tggttatcct
      301 catattctga tgggtgatgt aaaagtaact gtttcagcaa tgattgcttg cgctgtttta
      361 ttgcacccaa ataaattgtt tgcctccgtc gctggaacct cacttcccta tcaaacgtat
      421 cgtgattttg ctgagaataa gggagagttc cgtcctggcg cagaaaatgt tcctctatat
      481 gataaaaatg gcaatcccgt cacgacgctg aataaggcac ccatgattga cttcagctct
      541 aatgactcga ctggggttgc aacactggtt tcgccacagt atgtcgtcag cgttaaacac
      601 aatgttggtt atcaatatgt gaaatttggc tatgcagatg attcatccta tgcccttgtt
      661 gacagaaata accactggag agatttccac actccccgtc tcaacaaaat tgtcaccgag
      721 gttacacctc tggatataac caatgcgggt acagctaagg gaacctatca aaatgctgac
      781 cgtttccctg tgttctatcg agtaggtacg ggaactcaat atgtaaaaga tactaacgga
      841 aaaatcacct atttaatggg agcctactcg tataaaacag gtggaatagt aaatacgccg
      901 gctatatctg actggagttt tgtcacgaat accactaata ctccactctc aacctacggc
      961 actcccggtg acagtggctc tcctcttttt gcctgggatg cagaccaaaa taaatgggtt
     1021 ttgttagccg ttctcaattc atatgcaggg gtcaatggca atactaactg gtacacaata
     1081 attcctgctg gtgatgtaaa aaatacgatg aaacaagatg ccgatgcacc agtaaataca
     1141 aaacaaggtg aaggggatat tcattggtca tatgacgaaa aaacgggact tggttcatta
     1201 acccagggaa gcacatcatg ggaaatgcat ggaaatttgg gggcaacatg gcctgcttcg
     1261 cttaatagtg gtaaagatct aacttttcag ggcggcggta ctgtagtgtt agaaaatacc
     1321 gttaatcagg gggctggcac acttactttt gatgatgatt atattgtcaa gcccgttgat
     1381 actcagacat ggaaaggcgg cggaattatt gttaatggtg aacatcttgt tgactggcag
     1441 attaatgggg ttactggtga tagtttgcat aaactgggta cgggtacgtt aaaaattaac
     1501 ggtaccggtg taaacccagg aagcttaagt gtgggagacg gaaccgttat attggcacag
     1561 cgtgctgatg ataacggact gagccaggcg ttcagttctg tcagtattgt aagtgggcgt
     1621 cctactctgg tgctaaacga cgacaaacaa attaatccgg ataatatcaa atggggctat
     1681 catggcggca agctcgacat taatggaaac agtttgactt ttcataaact taacggggct
     1741 gatgatggag ctatactgac taacagtggc tccatggcta atgttaatct ggatttcaac
     1801 agcccagata caacagccac gattgctaat atctggcacg gccatttcac cggtaatcta
     1861 aatatcaata atgaggttac cgcaggaaca cagaatgatt tcgctattga tggtggagtg
     1921 aatgctcagg ggagtataac ccagcaaaat ggtcggctat ttatgcaagg acatccggtt
     1981 gttcacgccg tcagcagtca ggacgtagca aataagctaa aggcgctggg tgacaattca
     2041 gtactgaccc aaccagtgtc atttactcag aacgattggg aaaatcgaca gttcagtatg
     2101 gcggagctta acttacaaaa tgctgaattt aaccttgcaa gaaacgccag ccttaatacc
     2161 agaattaatg ccgagcattc taccgtgacg ttaggtagtg aggatcttta cattgatctc
     2221 aatgacggga atggcgtggc aaccaaacca acattgggta agtcaaaggc aaccgctgaa
     2281 gatgatcaga gtcgtttcaa tggacatgta cagttgcaac aaggatcggc actaactatt
     2341 aatgaacatt ttgccggcgg tattgatagt gcagatagtg cgacgacgat aacctctaca
     2401 gataccacgc ttaatcagct cagccgtttc acccaatctt ctttgtcatt aggtgaaggg
     2461 gcaaaattga ccgctacagc cggattactt tctgacggga cggtaagcag taatgccggg
     2521 gcgagccttt ccttgctatc agaccagcct ggaacgatgt attccgccca aagctgggag
     2581 cttagcgggc aagacacgag cttaaatgtt ggcgcgggtg ggatcatcac tggagatatc
     2641 aatgccaatg atgcggccag cattagcttt ggcacgactg atattaacca atccacaaat
     2701 tactatggaa atatcaacgc gccgttggca agtgtaacaa tgaaggacac tgcctggcag
     2761 gtaaacaagc agtcggtggc taagtcgctc actttgaatg gttcaacact gagctttaat
     2821 cgtttcgggc aagggggact gacgtcagat acgctggagg ccactaacag ttcgttcatt
     2881 atcaatgccg atggtaaagc agcagatacg gtgacggtaa accaggcatt gacagggggg
     2941 aataacacac tggttgttat tcctacgaca aattcagtaa aacagggcgg tgaccctgtg
     3001 tcattggtta ctgccccgaa aaatacacaa agcaatatat ttacacttaa cccggtttca
     3061 ataaatgcag ggttccatag ttttacaccg caacttgatg tgttggagac cgacgttaat
     3121 aagcaatggc gtctggaggg attttacata cagcctgata aagccgcttt gcggacaggc
     3181 aagagtttta tggatctcgg ttataaaaac tttattacag aaattaataa tctgaatgat
     3241 cgtatggggg acttacgcca tacccatggt gaaactgggg catgggctag acttaacagt
     3301 ggttcgggct cagctactga tggatttacc ggaagttata cgcacttgca aattggggca
     3361 gacaggaaac atataattga aggcggagag ttatttactg gagtaacggc gacatttaca
     3421 agcagtaata accggggaac tggttggtcg ggaagaacca aatcaacggg tattggtgtg
     3481 tacgcttcag ctatgtttga ctctggactc tatgtagata ctatcgggaa atatgtgcgt
     3541 catgacaatc attactcatc aagtgcgtta ggaatgccag aacaagatta tggctcgcac
     3601 tcctggtatt tgggcgctga ggccggttgg cgtttttcct tacctgacga aacatatata
     3661 cagccgcaaa cagagttgat ctacggtact gtatctgaga atcagtttgc ctggcaattc
     3721 aatgggggtg agatctatat gcaacgtaag cagatgcaac cattgattgg tcgcacagga
     3781 attgaatttg gcaaaacatt ccgcggcaaa gactgggaaa tgacagcgct gactgggatt
     3841 aattatcagt acgatttatt taagcctact gtgacagcgt tcaaggatct ggcaggtgat
     3901 acatatatta ataatggtaa agatagcaga gtggtattca atgtcggggt aaatactaaa
     3961 attaaagaaa acactaggat aagtctgaat gtggagcgtt ctgagttcgg ttcatacaat
     4021 attgataagt taataaatgc taatattcgt tatacattct gagc
//
LOCUS       DAOFSV010000256         4042 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.256, whole genome shotgun sequence.
ACCESSION   DAOFSV010000256 DAOFSV010000000
VERSION     DAOFSV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.256"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..857
                     /gene="gspK"
                     /locus_tag="QBH27_004661"
     CDS             <1..857
                     /gene="gspK"
                     /locus_tag="QBH27_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDQ7016869.1"
                     /translation="HLQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQL
                     ENGNTVKWQLRDAQHCFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIV
                     QSIADYIDADDSPRFHGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLI
                     PYVCVLPTSELSINLNMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFL
                     YWAQQDFSGVKPLVAQVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQI
                     YRRTLQLY"
     gene            872..2035
                     /gene="gspL"
                     /locus_tag="QBH27_004662"
     CDS             872..2035
                     /gene="gspL"
                     /locus_tag="QBH27_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDQ7016870.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLGSLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVGKSGKWLTRLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHSELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            2032..2493
                     /gene="gspM"
                     /locus_tag="QBH27_004663"
     CDS             2032..2493
                     /gene="gspM"
                     /locus_tag="QBH27_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDQ7016871.1"
                     /translation="MIKSWWAEKSTSEKQIIAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            2493..3165
                     /gene="gspO"
                     /locus_tag="QBH27_004664"
                     /pseudo
     CDS             2493..3165
                     /gene="gspO"
                     /locus_tag="QBH27_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
     gene            complement(3194..3670)
                     /gene="bfr"
                     /locus_tag="QBH27_004665"
     CDS             complement(3194..3670)
                     /gene="bfr"
                     /locus_tag="QBH27_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDQ7016872.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(3742..3936)
                     /gene="bfd"
                     /locus_tag="QBH27_004666"
     CDS             complement(3742..3936)
                     /gene="bfd"
                     /locus_tag="QBH27_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDQ7016873.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         1055 a         1025 c          897 g         1065 t
ORIGIN      
        1 atcatttgca acggcaatat gatattgagc tggcggagaa acttgcgctt gccagcctca
       61 cccaggacgt taaagataac gacagacaaa ccacgttgca gcagtactgg gcacaaccgc
      121 aacagttgca gctggaaaat ggcaatacag tgaagtggca gttacgcgat gcccagcact
      181 gttttaatct caatgcctta gcaaaaatct ctgacgcccc gctggcttcc cctgatttcc
      241 ccgcccaggt attcagtgcc ctgctgatta acgccggtat cgatcgcgga aacaccgatg
      301 agatcgtgca atcaatcgct gactatattg atgccgatga ttcgccacgc tttcacggcg
      361 cagaagataa cttttaccaa agccaaacgc cgcctcgcca tagcgccaat caaatgcttt
      421 ttctgaccgg tgaattgcgt cagataaaag gcataaccga aaacatctat cagcggctta
      481 tcccgtatgt ctgcgttctc ccgacctcgg aacttagcat caatcttaat atgctaacgg
      541 agaacgatat tccgctcttc agagcattat tcctcaataa catcacggat gctgacgctc
      601 gggtcttatt acaaaaaagg cccagggaag gctggctaac cacagatgca tttctttact
      661 gggcgcagca ggatttctcc ggcgtgaagc ccctggttgc tcaggtgaaa gggcatctct
      721 ttccctatag ccgctatttt acgctgagta cggaaagtat cagcgacgaa caatcccagg
      781 gatggcaaag ccatattttt ttcaaccgta aacagcagag cgcccaaatc tatcggcgta
      841 cgctgcaact ctactaatca taaggatcgt gatgcctgaa tcgttgatgg taatacgttc
      901 tttctcgacg ctgcgtaagc attgggaatg gatgacgttc tccgcagata gcgtatcttc
      961 ggtacatacg ctcacagacg atcttccgct ggggtcgctg gccgatcagc ccggtgccgg
     1021 aaatgttcac cttttgatcc cacctgaggg tcttttatat cgttccctca cgctgcccaa
     1081 cgccaaatac aaactcaccg cgcaaacatt acagtggttg gcggaggaga cattacccga
     1141 cgccagccaa aattggcact ggacggttgt cgataaacaa aacgaaagtg tcgaggtgat
     1201 aggcattcag tcggagaaac tcagtcgtta tcttgagcgt ttacatactg ccggactcaa
     1261 cgtgacgcgg gttttgccag atggctgcta tcttccctgg gaagtagata gctggactct
     1321 ggttaaccag caaacaagct ggcttattcg cagtgccgca catgccttca acgaactgga
     1381 tgaacactgg ttacagcatc ttgctaacca gtttccgcct gagaatatgc gttgttatgg
     1441 tgtagcgcct cacggcgttg cagcggccaa tccacttatc cagcatcctg aaataccatc
     1501 actgagtttg tattctgccg atattgcatt ccagcgctat gacatgctgc atggtgtttt
     1561 tcgtaaacag aaaactgtag gcaaatcggg aaaatggctc acccgcctgg cagtaagctg
     1621 tctcgttctc gccattcttt cttttgttgg cagccggggc atcgcgctct ggcaaacgct
     1681 taagattgaa gatcaacttc agcaacaaca gcaagaaacc tggcaacgtt atttcccgca
     1741 gatcaaacac acccacaact ttcgttttta ctttaagcag caactcgctc agcaatatcc
     1801 tgaagctgta ccgttgctct atcatttaca aacacttctg cttgaacact ctgaactgca
     1861 gttaatggag gcgaactata gccagaggca aaagtcgctg accttaaaaa tgagtgctaa
     1921 gagtgaagcc aatatcgatc gtttctgtga gttaacacag tcctggcttc cgatggaaaa
     1981 aacagaaaaa gatccggtca gtggtgtatg gacagtaagg aactcaggga aatgattaaa
     2041 tcatggtggg cagaaaaatc cacatcggaa aaacagatca ttgcggcact ggcggttctc
     2101 tcgctcggcg ttttttgttg gcttgcggtg ataaaaccga tagataccta cattgaagag
     2161 catcagtccc acgcgcaaaa aattaagaag gacatcaaat ggatgcagga tcaagcgagc
     2221 acccatggtt tactcggtca tccagcgctt acgcagccca tcaaaaatat ccttctcgaa
     2281 gaagcaaaac gtgagaattt gactatcacg ctggaaaacg gtccggacaa tactctgaca
     2341 attaatcctg tcacagctcc cctcgaaaac gtctcccgct ggctcactac ggcacagata
     2401 acatacggta ttgttattga agaccttcag tttactcttg ccggaaacga agagatcacc
     2461 ctcaagcatt tgtccttcag ggagcaacaa taatgacaat gttactgccg ctcttcattc
     2521 tggttggttt tattgcaggt tattttgtta atgccatggc ctatcacctc tcgcctttgg
     2581 aggataaaac ggcgttaacg tttcgccagg tattggttca tttctggcaa aaaaaatatg
     2641 cctggcatga tacagtgccc ctgatacttt gcgttgctgc tgctatcgcc tgcgccctgg
     2701 cacctttcac gcccatcgtg actggcgcac tctttctcta tttctgtttc gcactcacgc
     2761 tcagtgttat tgattttcgc actcagctcc tgcccgacaa actcacctta ccgctgctct
     2821 ggcttggtat ttaatgcgca gtctggatta attgatttac atgatgcggt ttacggcgcg
     2881 gtcgctggct atggggtgct gtggtgtgtt tactggggcg tctggttagt ttgtcacaaa
     2941 gagggattgg gctacggtga tttcaagcta ctggccgctg caggcgcgtg gtgtggctgg
     3001 caaacgttgc caatgatact gctgattgcc tcgctgggtg gcattggtta cgccatcgtt
     3061 tcacaacttc tgcaacgccg gactataacc actatcgcat tcggcccgtg gcttgcgctc
     3121 ggcagcatga taaacctggg atatctggcc tggatctctt attaaccggg agggttctcc
     3181 ctcccgacac ggctcaacct tcttcgcgaa tctgtgcttg cagataattt tgcagtccca
     3241 tcttctgaat cagatcaagt tccgtttcca gccagtcgat atggccttct tcatcgcgca
     3301 aaatttctat catcatatcg cggctgacgt aatcatgaac gctatcggca taaccaattg
     3361 cctcacgcaa attcttcgcg ccatccagct caagtgccag atcagaacgc agcatttcct
     3421 caacatcttc accaatgttc agtttgccca ggtcctgtaa gtttggaaga ccttccagaa
     3481 aaagaatgcg ctctatataa cgatcggcgt gtttcatctc atcaatggat tcatgatact
     3541 ccacatcatt gagacgtttg agaccccagt ttttaaacat ccgggcatga agaaagtact
     3601 gattgattgc gacaagctca tttcccaaca gtttgttgag ataatttata actttagtat
     3661 cacctttcat tttttgactc cttcgcttcc actctttgaa gcgtagaacg gcttacgagt
     3721 aagtcaaaaa acacactccg tttatgcgga ctccttaaac tccggcagtt gcattaattc
     3781 atcctccatc acttcacgcg cggcccggac acatttaccg cactgattgc cgaccggaat
     3841 gaattttttt aattgctgga acgagtgggg ggaaaactgg cgcacagcct gacgaatttt
     3901 tttatcgctg ataccattac aaagacaaac gtacatagag actcccgtat actttcttca
     3961 aaagtaaatg aaaatagttc ttatttcaat tgcaggaaga ctctttttta agcaaagaat
     4021 agaaatatga ataaaaataa tg
//
LOCUS       DAOFSV010000257         3947 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.257, whole genome shotgun sequence.
ACCESSION   DAOFSV010000257 DAOFSV010000000
VERSION     DAOFSV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.257"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(2..604)
                     /locus_tag="QBH27_004667"
     CDS             complement(2..604)
                     /locus_tag="QBH27_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016874.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(698..904)
                     /locus_tag="QBH27_004668"
     CDS             complement(698..904)
                     /locus_tag="QBH27_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDQ7016875.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKVGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(1100..2296)
                     /locus_tag="QBH27_004669"
     CDS             complement(1100..2296)
                     /locus_tag="QBH27_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HDQ7016876.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            3096..3236
                     /locus_tag="QBH27_004670"
     CDS             3096..3236
                     /locus_tag="QBH27_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HDQ7016877.1"
                     /translation="MNSHTRYKLTLAELEAFNSAVDNRLAELTANFTSACWLQSCHLS
                     VR"
     gene            3876..>3947
                     /locus_tag="QBH27_004671"
     CDS             3876..>3947
                     /locus_tag="QBH27_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDQ7016878.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVH"
BASE COUNT          952 a          982 c         1114 g          899 t
ORIGIN      
        1 atcaactgtg agcggcatca accgatgtac ggtatgccat aagcacgtac tgatccatca
       61 gatttgtcag tggttcactc tgaccctgac gtgcaatgat ccattgctcc agatcttttt
      121 ctgatttgtg tttcaggttt ttcgtccagt cgtcaaggct gtttccggtc attaagtcaa
      181 gccagcagag ccagaccagt ttgagacgta actccgtgcc gccaaactga cggaagaggc
      241 tgatcagttt gtttgccaga gaatgttgct tcaggcgctc acattcagac agcaggatcc
      301 cttccagcat cggatgatga accagcaggt gttcgctggt gcataaattc accaccagat
      361 catcatccag ggacggggag ctccgtttgt ccggaccata aatcagccgg tgcagactct
      421 ggggaacgat ggcaatctcc ggtttttcgc cattgaatga cacccacccg ggcgtcagat
      481 tatggaaata gtagatattc ggatgacaca cgccccatga tacggcgtgc cgggtgaagc
      541 cggtcagcca tttctggtag ttttcatgga gcagctcgta acgggtctga tactgttgtt
      601 tcattttcat attcctcacg ggggataaca ggcagcagag cgccgccggc aggccggaga
      661 ttgcgtctgc tttgggcgaa gggttagtgg ttaaaaatta cggacgggac tgtgcaatac
      721 gcgcctgcag ccaggcttcc acttcacttt tcagccagcg ggagctgcgc cccattttga
      781 tgggggcagg aaagccccca actttgatga gtttgtaaaa ccacttatcg gttaagccgg
      841 tcagttgagt gataaacgcc atgtcgacca tctggtcatc catcagcgaa actggggtag
      901 ccatgagaat gttcctccgg atgttgttaa accagccggt gtggtcatga aaacagcgcg
      961 ccaccttcac cggcataaca accggggaga aacgtaaaaa ataatcggga atctggctgt
     1021 ccggagataa acggggggat tgcgatggca gagccgtagc ggaatcccgg ggggtgcccc
     1081 tattaagttg tcaagcatgt tatgacgcct gcggggtata aaaagtcccg tcgcgcatca
     1141 tggcgaacag aacgtcgcag cgtcgtctcg ccagggcgat aagcgcctga ttgtgtcgtt
     1201 ttccctgact cattttgcgg gtgtagtaag ccctggagag cggatccctg agcgcggcga
     1261 aggccgacag gaacaacgcc cgtttgagag ctttattacc ccgtcgcgag ggatgctcac
     1321 cgcgtattga cgagccggat cgccgagtta ccggcgcaag gccagcataa gcagcgagat
     1381 gtgcgacaga ggcgaaggcg cggcaggcga cctcggtgag gagtctggct gcggtcctga
     1441 caccgactcc gggcatactg gtcaggaccg ggtaaagagg gtgagcaaga actcgctgtt
     1501 ctaccgcaag cgccacctcg tctctttgct tacgcagcgt gatgagctgg agtgccagac
     1561 gtggcagtac tacggcagcg gcattcgtgc cgggaacgac gacggtttgt tcggccagtg
     1621 cctgagctat gtctgctgca aggcgtttac ccagacgagg cgcaagtttg cagagctggg
     1681 ctgccagctt cttctcaccc agcgaagcga gtttttctgg tgagggatat cgctggagaa
     1741 gatcgagtac cgccgggtga tcaagtctcg gaccgagaac gcgctccagt gccggatgta
     1801 tctgggtcag aaggccgcgg atacggttgc tggcctgcgt tgtctgtgcg gcaagatcat
     1861 catcgaagcc gcagagcatg gagagttcgg cgatttgctc gtcagccagt ttcagcgtgc
     1921 gtagcgcgtg aggcagggta cgggcagctt cggcaatgat ggcagcgtca cgagcatcag
     1981 ttttagcttc accggcgtgt aagtcggcta tgcggcgcat ggccagtcca gggaggtatc
     2041 cgacaaggac tccttctgag cgggcaacgg cgacaggtaa cgcaccgatg gtagctggct
     2101 gatcaacaac cagcagtatc tgaccatgtt gtttcaggtc agatattagc gacctgagtt
     2161 tgttttcgtc gttgggtaat gctttatcga acaggcgttt acctgaacga ttaatggcta
     2221 cagcgtgatg cgtatcttta ccgacatcaa cgccgataaa gacctggacg gattcgtaat
     2281 cgctggattc ggtcattctg tctcccttgt atatgggtta agcagataac cacggggagc
     2341 aggtaccggc atccacgtta cagacggtcc cggcaaaagt gcctgacctg acccctatta
     2401 gcggttacca gcgccccacc agacccggtg acatcacccc ccggatcatg gacgactggg
     2461 ggcagtaatc atgccgggtc tggctggcta acaccccatt ataaggggta cgaataaagt
     2521 aacgggggtg cgaaaagatg cttccgcacg cacgcgcgat aaagagggga tagttttcgt
     2581 gaggacaggg agagaagcag gcgttctgat aattgcagag ttgttttcgg aggtggttac
     2641 agtgagtcag cccgttcctg gcgagcagga gactgatatc accgatggga taaggttgtc
     2701 tgattggtat tataaatata agaagtcatc agagagtaac gtaaaggatt tgccagaata
     2761 acctaagaga gtaaatctct ccggtaacag agcgctgtga agcattctgt atcagagtgc
     2821 aaatacatga acgctgatgc gtaaaatcag cagtgacacg ccagctcccc ctatgcccat
     2881 tcctgtataa gacatgaact gagcgtatta atgctcctgc cctgagactg agccggtgag
     2941 tgaggggctg agaaatccct cttgacactg ctctaaccag aaatacttcc ccggttgttg
     3001 cggtgcgtcc ggatccccga taacggaaca ctaaggctgt attgcgggac accaggtaag
     3061 tggcagcccg taatacgtca ggagacgcgg gaaaagtgaa ttcccacacg cgttataaac
     3121 ttacccttgc ggagctggaa gcctttaact ctgccgttga caaccggctg gcagaactga
     3181 ccgcaaattt tacgagtgcg tgctggcttc agtcttgtca cctgagcgtt cggtaacgga
     3241 ggcgcgggcg gtaatagact gattttcagg tattaccggc ttacggcgtg tggttgatat
     3301 tgaggggctg tgagtttcgc gtatttgtca gaaacccaga cgagttttcc tgtatgggac
     3361 gaaataaggc agcggcaata cctggaaaaa tttaatatgg tatgaaggag aacctgtcgg
     3421 ttacgcttaa tatacccggt acactgtcag ctcaggggat acatgaaccg ttatctcacc
     3481 agctacacag tatatacaca ctgacgctga cagtagtcag caggaccaca ataatcagtg
     3541 ccagcagtat ggtaacgata cggtctgtat tccggtaata gccgaagggg tcgacagaac
     3601 cacaatacgg gcaacacact gtattatgag taatcgcgtt tccacagtcc ctgcacttct
     3661 ttttgcgtat caccatcact ccctcctcac agccttaccc gtaaccggtt ttttacatta
     3721 aaaaaacaac gctgaaaatt caaccgtcag tccggagacg accgttcggg ttatcacaga
     3781 gtgcctgaga cagtgccctg ccggaggtca cttcagggac tctgcgcatt ttttacgacg
     3841 tggctattcc gtatgaacca tacggagatt taaccatgac ctacaaatac aaccccttct
     3901 ggcagcaacg tattcgtgag acagtgcggc acgcactgaa tgttcat
//
LOCUS       DAOFSV010000258         3921 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.258, whole genome shotgun sequence.
ACCESSION   DAOFSV010000258 DAOFSV010000000
VERSION     DAOFSV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.258"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            33..1412
                     /locus_tag="QBH27_004672"
     CDS             33..1412
                     /locus_tag="QBH27_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HDQ7016879.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            1415..2698
                     /locus_tag="QBH27_004673"
     CDS             1415..2698
                     /locus_tag="QBH27_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7016880.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            2688..3818
                     /locus_tag="QBH27_004674"
     CDS             2688..3818
                     /locus_tag="QBH27_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ7016881.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALFAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKLVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            3823..>3921
                     /locus_tag="QBH27_004675"
     CDS             3823..>3921
                     /locus_tag="QBH27_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ7016882.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRI"
BASE COUNT          733 a         1008 c         1268 g          912 t
ORIGIN      
        1 cttaattcaa cccgccgtac agagtcgaag ttatgagtta ctttttcgtc acgacgctac
       61 aggtcttttt ctgcattgcg cttctttccg gtgttctctg gagtcgaaac gatcctccct
      121 ctttacgtcc gctgacctgg acgctgctga ccgggctgat tgccggcgtg ctggcgggac
      181 tgtttattca cggcagccaa ccggtacaac tgctgctggt tggggcggag gtcatggtat
      241 cgctgctgtt tgtcctcagt ttctggtggg cttcaacgcg tatccgctat ctgtggcagg
      301 ggattctgat tttcggcgct gcccgccatt gggcgctgga ccctaatctg ggcgggctga
      361 ccagtacgca tgtgctgaat actgacctgc tgttaaacct gacggccgtc gttctcgctt
      421 ttgcgatcct ctgtctggcg ggcgtactct gcgcgatgct gctgcggcgt attcgcggcc
      481 tgtactggcc gttaacgctg attctgctgg tgatgatctg gctgccgctg agcggcaacc
      541 tgctgttgtt gctgatgaaa ttacaggttg ttccgctggg aaaatcactg ctgagcttcg
      601 tggcgaaagt cacaaataac acggcgctgt ataactgggc gggagccgcg ctgctgctgg
      661 ccctggcgct gtgctggctt ccggcgctgt tgcgggcttt tcgacagacg cgggaaaccg
      721 aagaaccgat tgctcaccgt ctggcgctgg cgcagcgtcg caacgctttg cggctgtggt
      781 tggtcacaat cggctgcgcg gtggtcgtta tcgcaggcca actgtggtgg gataaagtgg
      841 cctcgcagcc gccgcaactc tctgaagccg tgcccgtaac gctggggagc gatggcatgg
      901 tgcgcctgcc ggttgaacag cttcgtgacg gtaaactgca tcgtttcgtc tgggtggcgg
      961 acgatggcaa agcggtgcgc ttcttcgtga ttaaccgcta tcccgacaag ctgcgttttg
     1021 gcgtggtgtt cgacgcctgc ctgctgtgcg gcgatcaggg ttatgtcatg gagggcaatc
     1081 aggttatctg cgtcgcctgc ggagtgcata tttttattcc gtctatcggc aaggccggtg
     1141 gctgtaaccc ggtgccgatt gaaaactggc acaatgatga aaaagagctg gtgatccccg
     1201 gtaaagaact ggcgacaggg gtgaactact tctcgacggt catgactatt aaagtgaccg
     1261 atccggtcga tggttcaacg ttgaccaata cctcagccga ttacaaatac agctatggcg
     1321 gcaagacctg gttcttctcg tcagaggcta actatgaacg cttccgtgaa acgccggagc
     1381 agtttgttcc tgccgacatg agggaggaat aatcatgctg tggcggatgt tacgacaatc
     1441 ctgggggcgc aatttacgcc gcaaagtgct ggcgataatc accgtttttc tggcctccag
     1501 cctgatctcg gcgctgctgg cggtgtccat tgatatcggc gacaaaatgt ccagagagct
     1561 gaagtcttat ggtgcgaaca ttttgattga acccgccgga caggctgcgc tgcccgcgct
     1621 gtttagtgag agcagtaatc cgctttccgg tcaggatttt cttgatgaag ccgagctgcc
     1681 gaacatcaaa gatatcttct ggcgaaacaa cattgtcggg tttgcgccga tgctcggcgg
     1741 cgaagccagc gtggagggag aaccagtgcg gattctgggc actttcttca gtcagccagt
     1801 ggatattcct gatgaagagg gatatgagac ggggcagaaa acggtcagcc catactggca
     1861 ggtgaccggc gactggccgc aggaacccgc aggcgcagag ccgcagacct tagtcgggca
     1921 cgcgctggcg cggcaaatgg gctggaaacc cggtgataag ctgacattac gtaccgaagg
     1981 cgaagcagtg caggtcaccg tcagcggtat cctctccagc ggcggcgatg aagataacca
     2041 actggtgatg ccgctaagca cggtacagca cctgctgggg ctccccggaa aagtgcaggc
     2101 gattcgcgtc tcggcgctga cggtgccgga aaacgaactg tcccgccgcg cgcgggaaaa
     2161 cctggacgcg ctcaatgccg aagagtacga cctctggtat tgcacggcct atgtgtcgtc
     2221 gatcgcgcat cagctggaag aggcgatttc cggtgcggaa gtgcgcccgg tatggcaggt
     2281 cgcggcgtcc gaaggggtgg tgatcgataa aattcagttg ctgatggcgg tcgttaccgt
     2341 cgcggcgctg gtggcttccg caatggggat cgcctcgctg atgaccagca ccattatgga
     2401 acgcgccaaa gagatcggtt tgatgaaagc gctgggtgcg cgacagtggc aaattatgct
     2461 gctgttctac ctggaagcgg cgtccagcgg gctggctggc ggtgcgctgg gatgtattgc
     2521 cgggtggggg ctggcgaaag cgattggcgt catgctcttc gacgcgccgc ttaattttgc
     2581 ctggattgtg gtgccgtgcg tactggtgat tgcggtactg attgcgttga tcggcacctg
     2641 gttcccggcc cggcggattg cccggctgta tcctgtggag gtgctgtatg gccgctaaac
     2701 gtaccatgct ctggctgctg gtgtggcggg cgctgcgtct gcgttttcag cgggtcagcg
     2761 tggtgtttgc cgcgctgatg gtgggggcga ccatcgtgac ggcgcttttt gccgtctggt
     2821 tcgacattaa caccaaaatg agcgaagaac tgcgcacctt cggcgcgaat ttttacatcg
     2881 ggccggggca cggttccagt atgccgcagc aggagttgca gagcattctc gatcaggcgc
     2941 cgcaggggct ggtgcatggc gccagtccgt ggttgtacgg catggcgcgt accgagctgg
     3001 aaaaagtggt gatggttggc gtgtggttcg agtcgctgca aaaactggtg ccgtactggc
     3061 aggtcaccgg tagctggatt ggcgttagtt ttgacgaccg taatgcgatg atcggcgtca
     3121 aactggcgga acgcctcaac gtgcagccgg gcgacagcat cacgctggtg gatcacaatc
     3181 agcgtaagaa tttgcagatt aaaggcattg tggagtctgg cgatgcgacg gacaacatgc
     3241 tgatcgtcag cctcgacgtg gcgcaggcct ggctgcatca gccgggcaaa atcagccacg
     3301 gcttgctgag cgtcagtaac gatgtcggcc aggtggagaa ctatgccagc cggttgcagg
     3361 cgcagtatcc tgacctggag attcgtccgg tgcgtaaggt ctctgcttcc gaagggcagg
     3421 tgctggataa aattaaaggt ctgatgggac tggtctcgat cgtgattctg gcgctctctt
     3481 cgctgtgcgt gaataccacg ctgatggcga ttgtcggcga gcgggcgcgc gagtttgcgc
     3541 tgcaaaaagc gcttggttcc agcaacggcg atatcgtgcg gcaaattctg ctggaaacca
     3601 gcatcatcgc gctggcggcg gtggcgtgcg gctgggtgct gggatatttg ctggcgcaac
     3661 tgttgggatt aacggtgttt aatgccgcca tatcactgcg tctgccggtg ttgcccatta
     3721 ctctggtgtt gtctttactg gtcgccattc tggcggcgat tgttccggtg cgacgcgcgg
     3781 taagcgtcga gcctgctaaa gtgctgaaag gagagtagcg ccatgtccgt tacgcatatc
     3841 cctcaggtgg cgatcgaaac ccgccactta tacaaacggt tcggtgacgt taccgccctg
     3901 gatgacatta atctgcgcat t
//
LOCUS       DAOFSV010000259         3891 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.259, whole genome shotgun sequence.
ACCESSION   DAOFSV010000259 DAOFSV010000000
VERSION     DAOFSV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.259"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..765)
                     /locus_tag="QBH27_004676"
     CDS             complement(<1..765)
                     /locus_tag="QBH27_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ7016883.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGLLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKA"
     gene            complement(843..1643)
                     /locus_tag="QBH27_004677"
     CDS             complement(843..1643)
                     /locus_tag="QBH27_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDQ7016884.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1884..2798
                     /locus_tag="QBH27_004678"
     CDS             1884..2798
                     /locus_tag="QBH27_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ7016885.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELIADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(2795..3598)
                     /gene="dkgB"
                     /locus_tag="QBH27_004679"
     CDS             complement(2795..3598)
                     /gene="dkgB"
                     /locus_tag="QBH27_004679"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDQ7016886.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAITESGVPRHELHITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHSIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(3761..3837)
                     /locus_tag="QBH27_004680"
     tRNA            complement(3761..3837)
                     /locus_tag="QBH27_004680"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3801..3803),aa:Asp,seq:gtc)
BASE COUNT         1001 a          943 c          936 g         1011 t
ORIGIN      
        1 cgctttatgt gcttctagca tgatgatatc actggtgaaa gagccatcat tctgcaaggc
       61 aaaatacgtt ttcacctctg tcgatgcgct ctgttggtaa atgcgaatag cgtctactaa
      121 cgcttctggc gtacgcattc tcgctaccca tgaagaaaat tccagcggta acttatctgt
      181 aattaaatta tcaactatca gattggcttc actgattaac gtcaaccact caccgctggc
      241 gtggttccgt atgtgagatg tatcgcgtaa tgcttctacc gtctgtaacc agatgtcgcg
      301 cactgggtga cccggagaca ttacgtccat cacaatcagc agaccaccgg gtttcaatac
      361 ccggttcact tcacgcaatg ctgcaccaac atcatgccaa tgatgggcag aataacggct
      421 gataacaata tcaaatgcgt tatcggcaaa tggcaggctt tcgacataac cctggcgggt
      481 ggtgatattt ttcagttgcc gggcttcggc agcttgtgcc acgacatcca gcatatgggc
      541 agataagtca tacgccacca ccgtgctcac gttttgcgca gcgacaaagc tggcatgccc
      601 tgctccgcag cccatatcaa gtacacttgc atcaggataa tcagccagac gcaccgccag
      661 acgctgtaaa tcttgtccgg atgcatgtac ggtactggtc agatattcgc tggcttgaga
      721 gctaaactgc ttttctacgt ggtcatggtg ggattgtgtt gtcattgcgc tgtccttttg
      781 ttatttgagt ataaaagggc agcgccaaac gttggtctgc cctgtggcag acctgacata
      841 ccttatttat caggcttgcc gggactgaat tcaacgagta gcggattgtg atcggacgcg
      901 cgcgtaacca gtacagaagc ttcgctgacg ttcagaccac ggtagaaaac aaaatcgagc
      961 gggcgaccaa acgcccgacg gcgctgatca tcggtaaaac gcacctggcg cagcgacatt
     1021 tcccgcgcaa agcgatataa cgcgttcatc cttctacggc tccaggcatt gaaatctccc
     1081 gccataatga ccgggccact gtggtgagct atctgatcgc caataggaag taactgctta
     1141 ctatagacat ccacgcccag actgaagttg acggcgtgta tattaaccac catcaacagg
     1201 cgggtgtcag gtaacggata gaccgtcacc agtgccgact tcgccagacg caaaatgggt
     1261 tctcgttcac gtaacgggca gcaatacact ggatgtgccg ccgaaagggt cattacgcca
     1321 gaaggatgtt gtggcagcac gaaagcgggt acctgatcgg cggcaagata gttagcagtc
     1381 gcaaactgta ctaactctgg cgttgtctgc gcttcctgca ataacaccag atgtgcatct
     1441 ttgccgtagt tctttaatac cgacaaccat tcagcgcgtt gctgtttgta tatgttccac
     1501 accaggatcc gaatacgctc ttcggtactt aacggttctc caggtggtaa tgcctggccg
     1561 atgctcgcaa aagaccccgg cggtaagatc ctttccgcag gttgtccggc aacatagcgc
     1621 atggcatagg tgttttttcg cacttttgac tttgaaacct ctatcacgtc aacccttccg
     1681 cactcgggag ggttctactg ttatagggat tttagctcac tctttcaacg agcaattgta
     1741 tattgttatg taagcaagtg cttacaccgg tcaggatttt gctcattatt gagaacatta
     1801 aatcggatgg catatctcgc atcctgatgg tttaatgaca aataaattca aaatatctct
     1861 tctattttgc aacaggagca aaaatgaaag ccacgtcgga agaactcgcc atttttgttt
     1921 cagtcgtcga aagcggcagc tttagccggg cagcggaaca attagggcaa gcaaactcag
     1981 cggtaagccg ggcggtgaaa aagctggaga tgaaacttgg cgttagcctg cttaatcgga
     2041 ccacgcggca acttagcctg acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa
     2101 ttttgcagga gatggcagcg gcagaatcag aaattatgga gacgcgtaat acaccgcgtg
     2161 gactgttacg gatcgatgcc gcaactccag tggtgctgca ctttctgatg ccgttgatta
     2221 agccttttcg tgaacgctat ccggaagtca ctttatcgct agtctcctcc gaaacgatta
     2281 ttaatttgat cgaaagaaaa gtggatgtcg cgatacgcgc cggtacgtta acggattcca
     2341 gcttacgtgc caggccgcta tttaacagtt atcgaaaaat tatcgcctcc cccgattata
     2401 tttcccgcta cgggaagcca gaaacgatcg acgatttaaa gcaacatatt tgcctgggat
     2461 tcactgaacc cgcttccctc aatacctggc cgatagcctg tagcgatgga caattacatg
     2521 aggttaagta cggtttgtca tccaatagtg gggaaacact gaaacagctt tgcctgagtg
     2581 gcaacgggat tgcgtgtttg tccgactaca tgatcgacaa agaaatcgct cgcggagaat
     2641 tggtggagtt aatagcagat aaagtgttgc cagtagaaat gccattcagt gcagtctatt
     2701 acagcgaccg tgcggtaagt acgcgcatcc gggcttttat cgatttcctt agcgagcatg
     2761 taaaaacagc tcccggagga gctgtcagag aggcttaatc ccattcagga gccagacctt
     2821 ccgggctaac caggcggtcg ttgcaatcca gtgcggcgat cgctttttta tcttcggcat
     2881 caagctgtaa attttgtgcc ttaagattac tctccaggtt ttcacgttta gtagaagaag
     2941 gaattactga gtaaccttcc cccatagccc acgccagaat cacttgagcc ggagtcgcat
     3001 tgtgtttagc tgcaatacga gcaataacct catctttcag tgccttgcca tacgccagcg
     3061 tcatatagga agtaatatgg atactgtgct gtttagccca agcgaccact ttacggtttt
     3121 gcagataagg agagagttca atctggttag tagcgatgtt ttcagcgcca acagcagcaa
     3181 tcgctttttc catcaatggg atcgtaaagt tggaaatacc gatctcacgc gtcagccctt
     3241 gttttttggc ttccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg
     3301 acggccagtg gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt
     3361 tcagactcgg gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt
     3421 ggagttcatg acgtggcacg ccactttctg taatcgcctg acctactgcg gcttcgttat
     3481 catagatttg tgcggtatca attgcgcgat aatcaagttc aagcgccgtt ttcacagatg
     3541 aaataacaac gtcgtctttc agacggaaag tgcctaaacc aaatgcaggg atagccataa
     3601 tatgcctctt ttaatgctat tttcgttaac tgaggtgatt atgggcttca gactgttgcg
     3661 gaaaaatggc gtaaaacgca gaggattttt gcgattttgg caataataga aatacaaacg
     3721 aaaaaaaacc tcgagcgtta actcgaggct tcttagtaag tggcggaacg gacgggactc
     3781 gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc
     3841 gaattctttt acaaccaccg gttttatgac cggcttactg cttaatttga t
//
LOCUS       DAOFSV010000260         3880 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.260, whole genome shotgun sequence.
ACCESSION   DAOFSV010000260 DAOFSV010000000
VERSION     DAOFSV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.260"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(71..1577)
                     /locus_tag="QBH27_004681"
                     /pseudo
     CDS             complement(71..1577)
                     /locus_tag="QBH27_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768542.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1740..1892)
                     /locus_tag="QBH27_004682"
     CDS             complement(1740..1892)
                     /locus_tag="QBH27_004682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016887.1"
                     /translation="MQIVVNPGVITVVLYISEIISMATVDNFDVEELFVKQNSYPKVI
                     NVMKIS"
     gene            complement(1975..2193)
                     /locus_tag="QBH27_004683"
     CDS             complement(1975..2193)
                     /locus_tag="QBH27_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016888.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            complement(2325..3218)
                     /locus_tag="QBH27_004684"
                     /pseudo
     CDS             complement(2325..3218)
                     /locus_tag="QBH27_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3516..3764)
                     /locus_tag="QBH27_004685"
     CDS             complement(3516..3764)
                     /locus_tag="QBH27_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016889.1"
                     /translation="MNNSIPERFIFQCALFKNLEKEVFMTHGYVDSHIIAHALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         1188 a          861 c          716 g         1115 t
ORIGIN      
        1 ggcaaaaaat aatatttcat atgttaccgc gtcgatgaat tcgacgcggt tttttagcta
       61 acgggcggga tcagaactta tatttcaggc ccaccatcat tgcggtgtca ttgtagccat
      121 tatcacccag ctgcacgccc acattacccc agacgctggc cgccggattc agattgccat
      181 ttacgccatg tttcatttcc cccagattac gagccccgtc ttgttcgact ttcacaccgt
      241 tcattgagac agcaaaatct ttactgttat ggatcccgtt catttcaaca aaaggttcaa
      301 agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg aataaaggtg cggaaaccaa
      361 gtcggctctg gatgttgtaa tttgcgtcat tctcaatacg tgagccattg gcttccgtgt
      421 gcgcattatg atcgacgccc atccagatgg cctgtgccga ggtcgttctc aactgtccgt
      481 tgctgtcacg ccaggcatta tgcccgccaa tttgacatag ccaaagttgg cttgaacgct
      541 cctgttcagt tacaggatcg atatagcgcg tttcacccgc acgatcgttc agacgcgtaa
      601 caaaaagagt gttagcggct gccaggttgg cgacgtaact gccagcgtcg ggtcgcagga
      661 cgggggtagg cgtttctgtt gattctgaag tgacattgga aaaaaatcac tctctaccat
      721 tgtatatttt tgcaatgggg gctttagtgc catttgttgt aatagaactt ttttatatca
      781 atgttacctt gtcgaatagc aactgcataa cttccatcag ccacagtttc aagtaccaca
      841 taatctaaat taatatatgc tttattccca ttagcataag taccatagct attaatacca
      901 ttagttgtta tggaaccacc attcattttt aaatcagcac ggctaataac ttcgtaactg
      961 ttagaatcac aaacttcatc atctacagca tcgacacatg ttcgagactc tccaggactt
     1021 gaacatgcaa gaactcctac tgcattttcc ccataggtaa caatgttcgt tttatctgtg
     1081 gtttcaagac tggtgttatt ttccacagtc ccagaatcat taacctgact taaaaatccc
     1141 gctgaatatg ccccctcgtc tcgactgaat tatcaccatt cataacgatg gtgccaccaa
     1201 attcactgac tactgcgctg gctgttatcc ctccattttc tttgtcagat acgcttccag
     1261 aggtcttaat attgctatcg cttatatata atactttgtt ttggctatca acagaaatac
     1321 cataagatcc atcaccacta gtcatcacac tggcattatt tatatatcca gttacaccgt
     1381 catccacttt cacagcatgg ctttcgtctt aagtgattag atactctcca ttacttgtag
     1441 aagtattatt tttgatttca atcggtgatt cttcatcggt tatataggaa attacactat
     1501 aattatccaa caattgtgtt acccaccgcc agagcaataa gtcctaaact gaacccgggg
     1561 agttttttca gtttcataga ttaaccatcc attcattgga ttaacaaata ttcctgataa
     1621 aataacctat gagttcagat caccaaaacg gagggcaaag ataaaaacaa aagcacaata
     1681 aatttaaata gataaacagc gacaatattt aaatgataaa taagtcagca ttatcatttt
     1741 tagcttattt tcatgacatt gatcactttg gggtaactat tttgtttaac aaataactct
     1801 tcgacatcaa aattatcaac agttgccata gaaattattt cactgatata taaaacaact
     1861 gtgataacac cagggttgac aactatttgc acaataattc ctttccttca atgatccagg
     1921 ggttcactgc cagcaaccca ttcagccgca tactgacagt gagaaactga aagattagtg
     1981 ctgatccctg tggctgacga gtctttccct taatcgatta accagattga tcgccagcca
     2041 aacaagggca ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tgctgggttt
     2101 tgcaataagg atgaccagga gagtgatcaa cataaatgca atggaaaaga tgatatagag
     2161 agtgaaataa tccagattat tactattgtt catgctacta atggcaatcc agtcaaaact
     2221 ttcaaacaat tatagcaaac aaaagaatga acaatagtaa caagaggcaa cgagctaatt
     2281 agctgtctat aattacaccg taccatggcc ttactgatca attttcactc aaccacaacc
     2341 ttcaccttcg gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag
     2401 aaataataac cctgctgaaa tgtaatatta ttttggttca gatagtcaag ttgttctttc
     2461 gtttcgacac cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa
     2521 tccagaattc gggttgattc tgggtctgcg ttgacgcggc caacgaaact gtgatcaatt
     2581 tttatataat caatatgcag gtcatgcaga taagaaagcc ctgagtaacc agtaccgaaa
     2641 tcatccagcg cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt
     2701 tcatcaacat ttaatggttc acgctcggtg acttccagaa caaggtttaa atcgcggcgg
     2761 gtaaaactgt cacggtaatt taagcactcg tcgacaaacg tcggcgaaac aatatgcgag
     2821 gcgctaaaat taatcccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc
     2881 tgggtggcaa cctgattcat caggctttgc gtaagcggaa cgattaatcc gcatttttca
     2941 gcaagtggaa taaatgccgc aggtgatata tatccaccgt gaggttgttt ccagcgggct
     3001 aacacctcaa ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg
     3061 acgatctccc cattatttat agcccgtcgc aggatctctt caggcgttgc gcttttattg
     3121 aaatacctat caagaaggaa ggcagcggcg caggcgatta acagaataaa tatcaaaatc
     3181 ccaaagcctt tatcgataag tctgtagaag ctaaagagac gttcattcgc tcgcgctggg
     3241 gtctacagat agcatggtag agagcatcac aaatatccac gccgaagata aaattttctc
     3301 caacttcatc taacctatta attatttaga acaatgtagg cagaattaaa caaattaggc
     3361 gggatatcag gcgtcaagaa tggaaaacga gcgctctcca ttcctgtttg ccctctgata
     3421 ttgcggatat attaagaaag cataacgcct gaaatgctca ctttgcatca gcatagtgat
     3481 acagctgatg tttattcaaa aaccttactc aagttttaag agagcacgga ttccctgtca
     3541 ttaataatga tatccgttag tgtagtctta tgcatttcaa tgtactgcaa gatttgcaga
     3601 tagagatcag aaagaataac actggtctca tcttttaaac gcaaccgtaa agcatgagca
     3661 ataatatgac tatcaacata tccatgtgtc atgaacacct ccttctcgag gtttttaaac
     3721 aaagcacatt gaaaaataaa tctctctggg attgaattat tcatgtcagc ctcgcaggaa
     3781 tagtgaccca ataaaattat gctatgtata gtctaaagcg acagacaaaa ataagatgaa
     3841 tgagttttct ataaacttat actttataat taaaagttac
//
LOCUS       DAOFSV010000261         3811 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.261, whole genome shotgun sequence.
ACCESSION   DAOFSV010000261 DAOFSV010000000
VERSION     DAOFSV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.261"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            273..1373
                     /locus_tag="QBH27_004686"
     CDS             273..1373
                     /locus_tag="QBH27_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDQ7016890.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDISSQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDSALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1398..>3811
                     /gene="torZ"
                     /locus_tag="QBH27_004687"
     CDS             1398..>3811
                     /gene="torZ"
                     /locus_tag="QBH27_004687"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDQ7016891.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDHIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKNDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQP"
BASE COUNT         1052 a          851 c         1016 g          892 t
ORIGIN      
        1 atctgttgag cgggtgacag tgcgcatagc attgtgctag aaatattcta tatattcaca
       61 ttcattatgt gatttaaatt gccaaaactg acaaatatat attctaaata acaactgggt
      121 tattccttag caattaatga ttacattgta ataaatcata ttctttatcg attatttcag
      181 gcagtgttgt gccgaattaa gcaagtagtc aattcgttgt atatttattt atacaatggt
      241 atcggtgtcc tggaatttaa ttagaggaat ctatgcgagg gaaaaaacgc attgggttat
      301 tgtttttgct gatagcggtt gtggttggtg gcggcgggtt attgctggcg caaaaagcct
      361 tacataaaac gtcggatact gcattttgcc tttcctgcca ctcgatgagt aaaccttttg
      421 aggaatatca gggaactgtc cacttttcga accaaaaagg tatacgcgcg gaatgtgccg
      481 attgccatat tccaaagtca gggatggatt atttatttgc taaattaaaa gcatctaaag
      541 atatttatca tgaatttgtt agcggcaaaa tagacagtga cgataagttc gaagctcatc
      601 gccaggaaat ggccgaaaca gtatggaaag aattaaaagc aactgactct gcaacctgcc
      661 gtagttgcca ttcttttgat gccatggata tttcctcgca aagtgaatct gcgcagaaaa
      721 tgcataataa agcgcaaaag gacggcgaaa cttgtatcga ttgccataaa ggcattgccc
      781 atttcccgcc agaaataaaa atggatgaca acgcggcgca tgagctggaa agtcaggccg
      841 ctacttcagt tactaatggc gcacatattt atcctttcaa aacttctcgc ataggcgacc
      901 tggctaccgt gactcctggt accgatctca ccgtcgttga tgtcagtggt aaacagccaa
      961 ttgttcggtt gcagggttat caaatgcagg gcagtgaaaa cacgctctac ctggcggcag
     1021 gtcaacggct ggcgctcgcc acattaagtg aagaaggtat caaggcgcta acggtaaacg
     1081 gggaatggca ggctgacgaa tacggcaatc aatggcgtca ggcgtcttta cagggtgcgc
     1141 taaccgattc cgcattagcg gaccgtaaac cactatggca atacgctgaa aaacttgacg
     1201 atacctattg cgctggctgc catgccccta ttgccgccga ccattacacc gtcaatgcat
     1261 ggccgtccat tgccaaagga atgggtgcgc gaaccagcat gagcgaaaac gaactggaca
     1321 ttttgacgcg gtacttccag tacaacgcca aagatattac cgagaaacag tgatcctcgt
     1381 tgatcaggga ggaagttatg acattaacaa gacgcgaatt tattaaacat agcggtattg
     1441 ccgctggggc gctggtggtg acatcggctg caccgttacc tgcatgggca gaagagaagg
     1501 gcggcaaaat tctcaccgcg ggacgttggg gagcgatgaa cgtagaagtg aaggacggca
     1561 agattgtttc ttcgacaggc gcgctggcga agaccatacc gaactcctta cagtctacgg
     1621 cggcggatca ggtacacacc acggcgcgta ttcagcatcc gatggtgaga aaaagctatc
     1681 tcgataatcc actgcaaccg gcgaaaggtc gtggcgaaga tacctatgta caggtgagct
     1741 gggaacaagc attaaaactg attcatgagc aacacgatca tattcgtaaa gctaacggac
     1801 catcggccat ttttgccgga tcttacggct ggcgttcttc tggcgtgttg cataaagcgc
     1861 aaaccttact gcaacgttat atgaacctgg cgggcggtta ttccgggcat agcggcgatt
     1921 attcaaccgg agcggcacag gtgatcatgc cgcatgtggt cggttctgtg gaggtctatg
     1981 aacagcagac ttcctggccg ctgattctgg aaaacagtca ggtggtggtg ttgtggggca
     2041 tgaacccgct gaatacctta aaaattgcct ggagcagtac cgatgaacaa gggctggaat
     2101 acttccatca gctgaaaaaa tctggcaaac cagtgattgc cattgatcct atccgctccg
     2161 aaaccatcga attctttggc gataatgcca cctggatcgc accgaatatg ggcaccgacg
     2221 tggcactgat gttagggatt gcgcataccc tgatgacaca aggcaaacac gataaagtgt
     2281 tccttgaaaa atacactacc gggtatccgc agtttgaaga gtatctgaca ggcaagaacg
     2341 ataatacgcc gaagagtgcg gcctgggcgg cggaaattac tggcgtgccg gaagcgcaga
     2401 ttgtgaaact tgcagaactg atggcggcaa atcgcacaat gctgatggca ggctggggaa
     2461 ttcagcgcca gcaatacggt gaacagaaac actggatgct ggtgacgctg gcggcgatgt
     2521 tagggcaaat tggtacaccg ggcggcgggt ttggtttctc ttatcattac tccaacggtg
     2581 gcaacccgac gcgtgttggc ggcgtgctac cagaaatgtc tgcggcgatt gccggacagg
     2641 ccagcgaggc cgctgatgat ggcggaatga cggcgattcc tgttgcccgt atcgtcgatg
     2701 cgctggaaaa tcctggcggc aaatatcagc ataacggtaa agaacaaacg tatccgaata
     2761 tcaaaatgat ctggtgggcg ggtggcggta actttaccca tcaccaggat actaatcgtc
     2821 tgattaaagc atggcagaaa ccggagatga tcgtcgtttc tgaatgctac tggaccgcag
     2881 cagccaaaca cgcagatatc gtattaccga tcaccacatc gtttgagcgc aatgacctga
     2941 cgatgaccgg tgattacagc aaccagcata ttgtgccgat gaagcaggct gtcgctccgc
     3001 aatttgaagc gcgcaacgat tttgacgtgt ttgccgatct tgctgaatta ctcaaacctg
     3061 gcggaaaaga gatctatacc gaaggtaaag atgaaatggc gtggctgaaa tttttctatg
     3121 atgccgctca gaaaggtgcc cgtgcgcaac gcgtgacgat gccgatgttt aatgccttct
     3181 ggcagcaaaa taaactgatc gaaatgcgcc gcagcgagaa gaacgaacag tacattcgtt
     3241 atggtgattt ccgcgccgat ccggtgaaaa atgcgctggg tacgccaagc ggcaaaattg
     3301 agatttactc cagaacgctg gaaaaatttg gctataagga ttgcccggca cacccaacct
     3361 ggcttgcgcc tgatgagtgg aagggtaccg ccgacgagaa gcagttgcag cttctgaccg
     3421 cacatccggc acaccgttta cacagtcagc ttaactatgc ggaactgcgt aaaaaatatg
     3481 cggttgccga tcgtgaacca atcactattc acaccgaaga tgctgctcgc tttggtattg
     3541 cgaatggcga tctggtgcgt gtctggaaca aacgtggtca gattctgaca ggcgcggtgg
     3601 tgactgacgg gatcaaaaaa ggcgtggtat gcgtgcatga aggtgcatgg ccagatctgg
     3661 aaaatggctt gtgtaaaaac ggtagtgcga acgtgttaac ggcggatatc cccagctcgc
     3721 agctggcgaa tgcctgtgcc ggtaactctg cgctggtgta tatcgaaaaa tatacgggca
     3781 atgcgctgaa gttaacggcg tttgatcagc c
//
LOCUS       DAOFSV010000262         3660 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.262, whole genome shotgun sequence.
ACCESSION   DAOFSV010000262 DAOFSV010000000
VERSION     DAOFSV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.262"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            515..1864
                     /gene="ghxP"
                     /locus_tag="QBH27_004688"
                     /gene_synonym="yjcD"
     CDS             515..1864
                     /gene="ghxP"
                     /locus_tag="QBH27_004688"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDQ7016892.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2015..>3660
                     /locus_tag="QBH27_004689"
     CDS             2015..>3660
                     /locus_tag="QBH27_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDQ7016893.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEEN"
BASE COUNT          683 a          899 c         1063 g         1015 t
ORIGIN      
        1 gtctttgtct ttctctctat cccgctggta cacaggaggg tttcccccga cgtcaacaca
       61 cctcattcga gcacgtggtg gaggttccgg ttggtgttga tgctttaatt gtatgttacc
      121 ggcgtttctt cgccagtgta aaagtacact ttttagccgc aatatttttg tcatctcaga
      181 cgatttttta tcgcaatcct gaacggtata cggctcgata acgctgcaat cttgcgcatc
      241 gacgataacg tttgcgcatc aattgcctgg tttttcatcg tcaagacaat aaaagagaaa
      301 aaagcagcaa acttcggttg aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt
      361 taccacccgt tttgtatgcg cggagctaaa cgtttgcttt ttttgcgacg cagcaaattg
      421 tcgcaaacct ggagcaggaa gataacgttt cgctggcagg ggattgtccg ccacgcatct
      481 tgacgaaaat taaactctca ggggatgttt tcttatgtct acgccatcag cgcgtaccgg
      541 cggttcactc gacgcctggt ttaaaatttc acaacgtgga agcactgtcc gtcaggaagt
      601 ggttgccggg ttaacaacgt ttctggcgat ggtctactcg gttatcgtcg ttccaggcat
      661 gttgggtaaa gcgggcttcc cgcctgcggc agttttcgtt gcaacctgtc tggttgccgg
      721 actcggttct atcgtgatgg gtctgtgggc taatctgccg ttggcgattg gttgcgccat
      781 ctctctgacg gcgtttaccg cattcagtct ggtgctgggg caacatatta gcgtacctgt
      841 cgcgctgggt gccgtgttcc tgatgggtgt gctgtttacg gtaatttctg ccacgggtat
      901 ccgtagctgg attttgcgca acttgccgca cggtgtggcg cacggcacgg ggattggtat
      961 cggtctgttc ctgctgctca ttgccgctaa tggtgtcggt ctggtgatta aaaacccgct
     1021 tgatggtctg cccgtcgcgc tgggggattt tacgaccttt ccggtgatga tgtcgttggt
     1081 ggggctggca gtgattatcg gcctggaaaa actgaaagtc cctggcggca ttctgctgac
     1141 cattatcggt atctcaattg tcggtttgat cttcgatcct aacgtccatt tctccggcgt
     1201 tttcgccatg ccttcattga gcgatgaaaa cggcaattca ctgataggca gcctggacat
     1261 tatgggcgcg ctgaatcctg tagtcctgcc aagtgttctg gcactggtga tgacggcagt
     1321 atttgatgcc accggaacta tccgtgccgt cgccggtcag gcgaacctgc tggataaaga
     1381 tgggcagatc atcgacggcg ggaaagcact gaccaccgac tccatgagca gcgttttttc
     1441 tggcctggtg ggtgcagctc cggcagcggt atacatcgag tctgcggcgg gtacggcggc
     1501 gggcggtaaa accggtttga cggctatcac cgttggcgtg ctgttcctgc tgattctgtt
     1561 cctctctccg ctctcttacc tcgttccggg gtacgcaacg gctccggcgc tgatgtacgt
     1621 tggcctgctg atgctgagca acgtggcgaa aatcgacttt gctgattttg ttgatgcgat
     1681 ggcgggtctg gttacggcgg tattcatcgt gctgacctgt aacatcgtaa caggcatcat
     1741 gattggcttc gcgactctgg tgattggtcg tctggtttcc ggcgaatggc gcaagttgaa
     1801 catcggtacg gtcgttattg ccgtggcgct ggtgaccttc tatgcgggtg gctgggctat
     1861 ctaatcttac ataggattct gaaaacgggt ggcaatggct gcccgttttt attttctccg
     1921 cacattgtgt gagcttttgc gatattctga aaaaatgaga attcaggcat aacgcctttc
     1981 tcagatcata aaaaacatcg caaacaggga acgcatggaa atcttcttca ccatactgat
     2041 aatgaccctc gtggtctcgc tgtccggggt ggtcactcgt gtcatgccct ttcagatccc
     2101 gcttccgctt atgcaaatcg ccatcggtgc gctactggcg tggccgacgt ttggtttgca
     2161 tgtggagttt gatcctgaac tctttttagt cttgtttatc ccgccgttgc tgttcgctga
     2221 tggttggaaa acgccgaccc gtgaatttct cgaacatggg cgagagattt tcggcctcgc
     2281 gctggcgctg gtggtggtca ccgtggtcgg cattggcttc cttatttact gggtggtgcc
     2341 gggcattccg ctgatccccg cctttgcgct ggctgcggtg ctttctccga ccgatgctgt
     2401 agcgctctcc gggattgttg gcgaggggcg catcccgaaa aaaatcatgg gcattttgca
     2461 gggcgaagcg ttgatgaatg acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt
     2521 ggcgatgggg acaatgatct tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtggc
     2581 aataggcggt attctcgccg gttttgtggt gagctggctg tatggtcgct cgctgcgatt
     2641 cctcagccgc tggggcggtg atgaacccgc gacgcagatc gttctgctgt tcttgctgcc
     2701 attcgcttct tatctgattg cggaacatat tggcgtttcc ggcatcctcg ctgcggttgc
     2761 cgccgggatg accatcaccc gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct
     2821 gcgcgcaaac agcacctggg cgatgctgga atttgtcttt aacggcatgg tgttcctgct
     2881 gttaggtctg cagttgccgg ggattctgga gacgtcgctg atggcggcag aaatcgatcc
     2941 taacgtcgaa atctggatgc tgtttaccga tattattctg atatatgcgg cgctgatgct
     3001 ggtccgtttc ggctggctgt ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa
     3061 gccgatggag tttggttcgt ggactacacg agaaatcctg atcgcgtctt tcgccggggt
     3121 gcgtggggcg atcactctgg ccggtgtgct ctctatcccg cttctcttgc cggatggtaa
     3181 cgtcttccct gcgcgctatg agttggtgtt cctggcggct ggcgtcattc tcttctcgtt
     3241 gtttgtcggc gtggtgatgt tgcctattct gctacaacac atagaagtcg cggaccattc
     3301 gcaacaattg aaagaggaac gtattgcacg agcggcaacg gcagaagtgg cgattgtggc
     3361 gatccagaaa atggaggagc gtttggcggc ggataccgaa gagaatatcg ataaccagct
     3421 gctcacagag gttagttctc gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa
     3481 tgatgtagaa agttcaatcc tggaagagaa cctggagcgt cgcttccgtc tggcggcatt
     3541 gcgttctgaa cgtgctgaac tgtaccacct gcgcgccacg cgggagatca gtaacgaaac
     3601 gctgcaaaaa ttactgcacg atctcgattt gcttgaagcg ttgctgattg aggaaaatca
//
LOCUS       DAOFSV010000263         3642 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.263, whole genome shotgun sequence.
ACCESSION   DAOFSV010000263 DAOFSV010000000
VERSION     DAOFSV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.263"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..442)
                     /locus_tag="QBH27_004690"
     CDS             complement(<1..442)
                     /locus_tag="QBH27_004690"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDQ7016894.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFH"
     gene            complement(439..3531)
                     /gene="ebgA"
                     /locus_tag="QBH27_004691"
     CDS             complement(439..3531)
                     /gene="ebgA"
                     /locus_tag="QBH27_004691"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDQ7016895.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     DFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSQASYEFGAGFFSTNLHSENKQ
                     "
BASE COUNT          817 a          940 c          988 g          897 t
ORIGIN      
        1 tatggaaata accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc
       61 agataaaccg atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta
      121 cggtttctcc gcagcctttt aaatattcgc ggtcagtttc atcacgataa tattccacta
      181 cctgtaatgt ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca
      241 cttcaaaata gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtcc gtctccacgc
      301 ggtaagtcaa tgagtcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt
      361 caatcgcttc aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt
      421 ctaagttatc gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc
      481 tgcgccgaac tcatacgacg cctggctttg cgcggtagct tctccgccag aaaccggcag
      541 caacgtaaag ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct
      601 gccccaggag ttggagccga ggccaggcaa ctggtggtcg agattcaggg tgatgtcatc
      661 actgcgctgc agctcgttac agtgctgggc tgcgtggatg ttttcctggg tgtagtgcca
      721 ggcactgaag ttaattgggc gctgcggaac aaccagcaga ccattgccgt ggcggttagt
      781 cagtgccgtc cagcggacat gctgacggtt gccgttgttc tgtgggaagg gatagttctc
      841 gaacatggca tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc
      901 gtagttttcg cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc
      961 catggtgaaa ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag
     1021 cgccacgtta acctggccat cggcagcgat gcgccagatg taggtgcagc gcatcccgaa
     1081 gtcaaacacc ggtggggcaa taaccgtgcg gctgatgatc aacacttcac catcgctctg
     1141 ttctacggca aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacag
     1201 cccttcgtac tcctgcttgt ggttgtcgat cattggcttg aagaagttga tctttggctc
     1261 gcgggtcagc agcgactcgc cattcacctg ccaggatgtc ggtttgccac tcatttttga
     1321 gaaggtgatt gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt
     1381 cagcggacgc gcattatttg gcgcgaaagg tacaggctgc gcggtatttt ccttcagcgg
     1441 gaactgataa gtagcgatag ggtgtccggc ttcgctgtag cgggtgcggg aatctttggt
     1501 caccgtaatg ttgagaaacg cttcacgagc gtccagttgc ggcagcgtga tctgcaaggg
     1561 ggcttcgctg ttcggtgcaa cgtcgcacag tttaatctgc tgcgtcgcga gcgtttcacc
     1621 ttcggcgcgc acctctgcgt gcagggtgta gtcatccagc gtggtaaacc acagtttatt
     1681 ttcgactttc aactcgccgc gagtcagatc caacgcgtgg atttttaccg gcgcgataac
     1741 ctgtttgtac tctttcaggc ccggcccagg cgtctgatcg gaatagatca aaccatcaag
     1801 acagaagtta tagttgttgg gatagtcgcc gtagtcgccg ccgaatttat accagacatt
     1861 gccgttgtca tcctgcgcct ggatcccgtg gtcgcaccat tcccagacat aatgtccctg
     1921 aatgcaatcg tgcttataga agacgttctg gtactccgtc agcccgcccg gtccgttccc
     1981 catcgcatga gcatattcac agatgatgcg cggcttcgga tgcgggtatt caccaaactc
     2041 attcatcagc ggcacgcggg tgtacatggt ggaaataata tcgaccactt cagcatcgcg
     2101 atcttcttcg tagtgcacca gtcgcgtgtc atccagcgcc ttagccgcat ggtacatcgc
     2161 gcggatgtta cagccatagc cggattcatt gcccagcgac cagatgatga tcgacggatg
     2221 gtttttctgc gcgtgaatat ggcgaacaat gcgctcgacg tagacgtttt cccactgcgg
     2281 atcgtcggta atacggctga tatcgccgac attagcaaag ccgtgcgatt cgacgtcggt
     2341 ttccgccatc acaaacaggc cgtagatatc acacagttcg taaaaacgcg gatcgttcgg
     2401 gtagtgagcg gtacgcacgg agttgatgtt gtgctgcttc atcaactgga gatctttctc
     2461 gacgcgatcc attccaacgg cgcggccttt gcgatgatcg ttgtcgtgac ggttgacgcc
     2521 gtgcagcatc acataacggt tattgatcca gaacagaccg tcgcgcactt tgatatcacg
     2581 gaagccaacg cgttgtggca ccacttccag aacgttgccg tcggcgtctt tcagcgtcat
     2641 gaccagatgg taaagataag gggattctgc tgaccattgc tgcggctgtt cgacagtaaa
     2701 atcaaagcgg gcgctggtca gtttttcaat tgccaaatga tcaatggcgc tgctgtgcac
     2761 cacgcgttcg ccatcaaaca gggtatattc cagcgtcgtg acgacagggg aggcggcgag
     2821 attttccagc accacttcgc aggaaagtgt ggcatcgcaa taggcttcgt caaagtcggt
     2881 gcgcacggtg aaatcgttaa tgtgcgtgag ttgttttccg atcagataaa catcgcggaa
     2941 gatccccgcc gaccaccaca tatcctggtc ttccacgtag gtagagtccg cccattgcat
     3001 cacgcgcaca cacaacaggt tgtcgccggt tttaaccatc gcgctgatgt caaactctgc
     3061 ggtcaggcga ctgcctttgc tgaaccccac atactgaccg ttaacgtaga cttcaaaata
     3121 ggtttcgacg ccgtcaaatt taatcagcgt ctgtttaccc tgccagccgt cgctgagggt
     3181 gaaaatacgt tgataggcac cggttgggtt atcgctgggg acaaacggca catcgatggg
     3241 gaacggaaaa ccttcgtcgg tatattgcag tttgccgtga ccttccattt gccacatggc
     3301 ggggacggta atatgccccc agtcagccat taactcagag gtgaaggctt ctggtacttg
     3361 cagcggatgg tcaaaaaagt ggaaattcca ctgaccgctt aaggacagaa acaggctgct
     3421 ggtttcgcgg gcaaaggtac gcgcttgcgc aacagaatca tatgaaaaaa agtacgcacg
     3481 cggcgcaagt cggttttcgt gggtgagctg aatgttttcc cagcgattca tagggcctcc
     3541 cggtagagag aaatcggaac catttatggt gatggtagtt tgtgtaattg ctggggagtt
     3601 tagtaaaatt tttactaaaa aatagcaggt aaatgcattt ac
//
LOCUS       DAOFSV010000264         3641 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.264, whole genome shotgun sequence.
ACCESSION   DAOFSV010000264 DAOFSV010000000
VERSION     DAOFSV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.264"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            126..1823
                     /gene="maeA"
                     /locus_tag="QBH27_004692"
     CDS             126..1823
                     /gene="maeA"
                     /locus_tag="QBH27_004692"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDQ7016896.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="QBH27_004693"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="QBH27_004693"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDQ7016897.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATKMGADLAINSRTEDA
                     AKIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLEDINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3153..3430
                     /locus_tag="QBH27_004694"
                     /pseudo
     CDS             3153..3430
                     /locus_tag="QBH27_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3430..>3641
                     /locus_tag="QBH27_004695"
     CDS             3430..>3641
                     /locus_tag="QBH27_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDQ7016898.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNL"
BASE COUNT          907 a          896 c          977 g          861 t
ORIGIN      
        1 aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt ggtgccgacg
       61 ccctggcggt aatgcaaaga cgataaaagc ccccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tattgctgga atttccgttg ttgaataaag gcagcgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga cactaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgccagt catttacacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggtgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt accaaaccat aatatcaaag tgattgtggt gactgacggc gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agtccggcct atacccttcc ggtggtgctg gatgtcggca
      721 ccaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtact gttgcagttt gaagacttcg cacaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcaccgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggcggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac cgacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaatct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gtaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacttcacg cgtggaagcc acaccgcagg acattatcgc ctggactgaa ggtaacgcgc
     1441 tggtcgccac tggcagtccg tttaatccag tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcgggct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacacttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcctttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa
     1741 cgtctgccga agctctgcaa caggccattg atgataattt ctggcacgcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgcg cccggtagtg agggctaccg ggcaattttc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga aggctgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtgat tctgggacac gaaggtattg gtgtggtggc agaagttggc
     2161 cctggtgtta cttcattaaa accaggcgat cgtgccagcg tggcgtggtt ctatgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcggccagca gcattacctg tgcgggggtc
     2401 actacctaca aagccgttaa gctgtcaaaa attcgtccag gacagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcga agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgaacag ttaaaactgg caaccaaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa agccggtggc
     2641 gctcacgcag cggtggtgac agcggtagct aaagctgcgt ttaactcggc ggtggatgct
     2701 gtccgtgcag gtggtcgtgt cgtggctgtc ggtctgccgc cggagtctat gagcctggac
     2761 atcccacggc tggtgctgga tggtattgaa gtggtcggtt cgctggtcgg cacgcgtcag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag aggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcgctaagag gtctttgctg cgactgccat gttcgggtcg
     3001 cagcctgttt cgcgagacgc tttccgtaat ttcactcttc ttcttttcct cccccatatt
     3061 tcccctcaca attcgcgaaa acgtagcgaa atttgttgcg ctgcacggaa caatagtgat
     3121 agtgttcctt ttgagggaac aaggatgaag agatgataat gaatttcaga cacaagggat
     3181 tgcgtgactt gtttcttctc ggcaaaacct ccggcgttat accgacgcaa gtcaaacgat
     3241 tacgccacca ctcgctgtga ttgatgcagc atgctgtctt gctgatatcg atatgcccgg
     3301 ttaccgacta catccgttaa gcggcgatcg cgatggaatt tgggcgatat ctgtctcggg
     3361 caactggcga atcacatttg aattcgtcaa tggcgatgca tacatactgg attacgagga
     3421 ctatcactga tgaaaatggc caatcatccc cgcccggggg acattattca ggaatcactg
     3481 gacgaactta atgtcagcct gcgcgagttt gccagagcaa tggaaattgc gccctcaacg
     3541 gcaagccgat tgctgaccgg aaaagcagct ttgacgccag aaatggcaat aaaactttcc
     3601 gtggtgatcg gcagttcgcc gcaaatgtgg ctgaatctgc a
//
LOCUS       DAOFSV010000265         3543 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.265, whole genome shotgun sequence.
ACCESSION   DAOFSV010000265 DAOFSV010000000
VERSION     DAOFSV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.265"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            67..3540
                     /locus_tag="QBH27_004696"
     CDS             67..3540
                     /locus_tag="QBH27_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDQ7016899.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDITINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEI
                     TQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTP
                     AGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPA
                     FSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGITIS"
BASE COUNT          841 a          918 c         1086 g          698 t
ORIGIN      
        1 ggcgaaggat atccgacttc tgtacggaat ggcaagtggc ggttaattta ttcaggggaa
       61 ggctgtatgg gaaaaggtgg cggtaaggca cacacgcctc gtgaggcgaa ggataatctc
      121 aaatccacgc agatgatgag cgtgattgat gcgattggtg agggaccgat agaaggtccg
      181 gtgaagggac tgcagagtat tctggtgaac aaaaccccac tgacggacac ggacggcaat
      241 cccgtgatac acggtgtgac ggcggtctgg cgcgccgggg agcaggagca gacaccaccg
      301 gaaggctttg agtcctccgg agctgaaacc ggactgggcg tggaagtgac gaaggcaaaa
      361 ccggtgacgc gcaccattac gtccgcgaac attgaccgcc tgcgggttac cttcggggtg
      421 cagtcactgg tggagaccac ctcaaagggt gaccgtaacc cggcatccgt ccgcctgctg
      481 attcagttac agcgtaacgg taactgggtg acagaaaagg acatcaccat taacggcaag
      541 accacctcac agttcctggc ctcggtgatt ctggataatc tgcctccccg gccctttaac
      601 atccggatgg tcagggagac ggcggacagc accacggacc agctgcagaa taagacgctg
      661 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggccattgtg
      721 gggctgcagg tggatgcgga gcagttcggc ggccagcaga tgacggtgaa ctaccatatc
      781 cgcggtcgca tcatccaggt gccgtcaaac tatgacccgg aaaaacgcac gtatagtggt
      841 atctgggacg gcagtctgaa accggcatac agcaacaacc cggcctggtg tctgtgggac
      901 atgctgactc acccgcgcta cggcatggga aaacgtctgg gggcggcgga tgtggacaag
      961 tgggcgctgt atgccatcgg gcagtactgc gaccagacgg tcccggatgg tttcgggggg
     1021 accgagccgc ggatgacctt taatgcgtac ctggcacaac agcgtaaggc gtgggacgtt
     1081 ctcagtgatt tctgctctgc gatgcgctgt atgccggtat ggaacggtca gacgctgacg
     1141 ttcgttcagg accgcccgtc ggatgtggtg tggccgtaca ccaacagcga tgtggtggtg
     1201 gatgataacg gcgtgggatt ccgctacagc ttcagtgccc tgaaggaccg gcacacggcg
     1261 gtggaggtga attacaccga cccgcagaac ggctggcaga cctccacgga actggtggaa
     1321 gacccggaag ccatactgcg ctacggacgc aacctgctga agatggacgc gttcggctgt
     1381 accagccgcg gtcaggccca ccgtgccgga ctgtgggtga taaagaccga actgctggaa
     1441 acgcagacgg tggatttcac gctcgggtct caggggctgc ggcacacacc cggtgacatt
     1501 attgaaatct gtgataatga ctatgccggg accctgaccg gcggacgtgt cctgtccatt
     1561 gatgctgcca cccgcaccct gacgctggac cgtgaggtta ccctgccgga gacaggtaca
     1621 tcggcggtga acctgattaa cggcagcggt aagccggtga gtgtggacat caccgcacac
     1681 cccgcgccgg accggataca ggtcagtacc ctgcctgatg gtgtggagac atacggggtg
     1741 tggggactct ccctgccgtc actgcgccgt cgcctgttcc gctgtgtctc catccgggaa
     1801 aacacggacg gcacctttgc catcacggcg gtgcagcacg taccggaaaa agaagccatc
     1861 gtggataacg gtgcccgctt tgagccgcag tcaggttccc tgaacagcgt catcccaccg
     1921 gcagtgcagc acctgacggt ggaggtgagc gcagctgacg gccagtatct ggcgcaggcg
     1981 aaatgggaca cgccgcgggt ggtgaagggt gtgcgcttca gtctgcgcct gaccagtggt
     2041 aagggaacgg atgccagact ggtgaccacc gccatcaccg cagacacgga gcaccgtttc
     2101 agcggcctgc cgctcgggga atacaccctg acggtgcggg cgataaacag ctatggccag
     2161 cagggtgaac ctgccaccac caccttccgg attgccgcac cggcagcacc gtcgcggatt
     2221 gagctgacgc cgggctattt tcagataacc gcaacgccac atcttgccgt ttatgacccg
     2281 acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag gcaggttgaa
     2341 accgcagccc gctatcttgg ctcggcgctg tactggatag ctgccagtat caatatcaaa
     2401 ccgggccatg attattattt ttatatccgc agtgtgaata ctgttggcaa atcggcattc
     2461 gtggaggctg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaagga
     2521 gaaatcggga aaacacatct ggcccaggag ctgtggacgc agattgataa cggtcagctt
     2581 gcgccggacc tggctgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcag
     2641 accgtcaata aaaaactgga aaatcagagt gcggcaatcc agcagataca gaaagttcag
     2701 gttgatacaa ataataacct gaacagcatg tgggccgtga aactgcagca gatgcaggac
     2761 ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc aggaatgcag
     2821 agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa tggcaacaca
     2881 aagccgatgt ttgttggtca gggcgatcag atatttatga atgaagtgtt cctgaaatat
     2941 ctgacggctc ccaccattac cagcggcggt aatcctccgg cattttccct gacaccggac
     3001 gggcggctga cggcgaaaaa tgccgatatc agcggtaacg tgaatgcgaa ctccgggacg
     3061 ctcaacaacg tcacgattaa cgagaactgt cgggttctgg gaaaattgtc cgcgaaccag
     3121 attgaaggcg atctcgttaa aacagtgggc aaagctttcc cccgggactc ccgtgcaccg
     3181 gagcggtggc catcaggaac cattaccgtc agggtttatg acgatcagcc gtttgaccgg
     3241 cagattgtta ttccggcggt ggcattcagc ggcgctaaac atgagaaaga gcatactgat
     3301 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc
     3361 gcgctggata atacgctgat ttacagtggt gttattgata tgcctgccgg tcacggtcac
     3421 atgacactgg agttttcggt gtcagcatgg ctggtaaata actggtatcc cacagcaagt
     3481 atcagcgatt tgctggttgt ggtgatgaag aaagccactg caggcatcac gattagctga
     3541 att
//
LOCUS       DAOFSV010000266         3444 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.266, whole genome shotgun sequence.
ACCESSION   DAOFSV010000266 DAOFSV010000000
VERSION     DAOFSV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.266"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..342
                     /locus_tag="QBH27_004697"
     CDS             <1..342
                     /locus_tag="QBH27_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDQ7016900.1"
                     /translation="TLHYVWAREFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAG
                     MIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKAHC
                     TGERNFGCSRG"
     gene            508..909
                     /locus_tag="QBH27_004698"
     CDS             508..909
                     /locus_tag="QBH27_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016901.1"
                     /translation="MYAKSFLALDSNGRLTGARTVQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            897..1307
                     /locus_tag="QBH27_004699"
     CDS             897..1307
                     /locus_tag="QBH27_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDQ7016902.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPSCGTGVYSREGGLQEGNWKDRN"
     gene            1591..3210
                     /locus_tag="QBH27_004700"
                     /pseudo
     CDS             1591..3210
                     /locus_tag="QBH27_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3254..3403
                     /locus_tag="QBH27_004701"
                     /pseudo
     CDS             3254..3403
                     /locus_tag="QBH27_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310931.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
BASE COUNT          851 a          822 c          968 g          803 t
ORIGIN      
        1 actctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa acactatcat
       61 ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gtgattaccg cgctccggga
      121 tcactggccg gaatgattga acaggcctgg tgcagtgccc tgggggtgga tgccggatgc
      181 catgccacgc tggtgcattt tccggcctgg ccggcggtgt ggctggcgcg taatgatgac
      241 accggctttc agcaagtgct ggaacgtgct gactatctgg cgaaggagca taccaaagct
      301 cactgcaccg gtgagcgcaa ctttggctgc agtcgtggct gagccagaca gacgagttat
      361 ctgacttcag tgtgatgacg tgttcatggc caccacactg attttccctt atgagactga
      421 actgaaaccg ccattcacac ctgaccacgc tgcccgtacc gggacggctg ttgcctgcgt
      481 gtcgtctgac ggtaaggaac atatactatg tacgcaaaat cctttctcgc tcttgatagc
      541 aacggacgtc tgacgggcgc ccgtactgta caggccgcac cttatgctca ctacacctgc
      601 cacttgtgtg gcagtgcact cagataccat ccgcaatacg acactgaact tccctggttt
      661 gaacacactg acgacaggct gacagagcac ggtcaacagt gcccttatgt caggccggag
      721 cgcagagaaa tacagttgat taaacgtctg cagcaattcg taccggatgc cttacccgtg
      781 gtgcgtaaag ccagctggca ctgcagacaa tgtcaccacg attattatgg ggagcggtac
      841 tgcacacact gccagaccgg aggattcagt attccccgga caactcagga ggaaatatgc
      901 gaattctgaa ctgttatatg gcgaatgaca gcaaaggcca ttttgttacg gcgaaagaag
      961 cagcgaagca caaccgccag gacgttttgt gctgtgtgtc ctgtggatgc ccgttaacac
     1021 ttcagcgggg caatgacgga caaccaccgt ggtttgaaca tgaccagatg actgtcgctg
     1081 agaaaatcct gctgcgatgc acctggcttg acccggcaga gaaagaggcc cgtcgtttgc
     1141 atctgcaggg tatgacggtt ccggattata cggtgaaggt gagaaagtgg ttttgtgtga
     1201 tgtgtgacga agattatgaa ggagaaaagt gctgcccgag ttgtggtacc ggggtataca
     1261 gcagggaggg ggggctgcag gaaggcaact ggaaggatag gaactgagcg gttcagtcgc
     1321 ggaaatcagt tgtagagctg taatgatatc tccgggagtg ctgcgttggt aggggcatcc
     1381 cgggatagag aaattttagc actttgccct gaatgattgt agacgtcccg ggtactcgta
     1441 ccggtctgag ttattacagt tagatattca ctttttctcc cgcatacttc acatctgtca
     1501 acgacggatg aaaagtggtt cttccgcaac ctgggtggaa gaaaatggat gaaactttct
     1561 ggcgaacaag gatctaagct gttttaagtt atgggcaacg caatgcactc tcttaagaca
     1621 cttctacagt taccttgcgg atggcgatgc agtcgacaaa ttattagctc tgacggtata
     1681 accctccatc tccacggaaa acgcaaaaca gcacaatgtc ctgaatgctt taagcgtagc
     1741 gactctgttc atagttgtcg tcggcgccgg atacagcatc taccctgctc agggcagacg
     1801 ctatggcttg tattttccgt ccgccactgg tactgccgta acccggcttg ttaacgtaaa
     1861 atttttgccg agtcgcttgc tcccttcgcc ggttcacacc agcagtcttc acaggcgtta
     1921 caaaatttac aacgtcaact gggattaata gctggaggtg aggctggaaa acgggctgca
     1981 acggcagcgg gtctccgttg cagtgctgat actcttcttc gcagggttat caataccccg
     2041 gagacgaaac agtcaggcgc gcctcatgtc ggtattgatg agtgggcgtg gcatcggggc
     2101 cactgctgcg gtatgttaat agtcaatctt gatactcacc gtcccctcgt cctgcttccc
     2161 ggccgtgatc agcgtacgct ggcgacctgg ttcagaaaat atccggaaat acaggttgtc
     2221 tcgcgtgatc gcagtggagt ctatgcgaca gcagcacgtg aaggtgcacc tcaggccaga
     2281 caggtggccg atcgatggca cctgctaaaa agtattggtg atgagcctga acgaatgatg
     2341 tacagacata tgcctctgat acgtcttgtt gtcagagagt tatcactgaa gaaatcacct
     2401 gagccagaaa tatctgtgcc tgtagcatcg ctccgtcgtc cggaacgcct taaacagcaa
     2461 acccgcaaaa aacggcatca gcattggaca gaggttatgg ccctgcataa caagggatgt
     2521 agtttcaggg aaatatcccg tattacaggc ctgtcgcgag tgacagtcag tcgctgggtg
     2581 cgttcaggaa cattccctga aatgtcaacc cggcctccaa agcgagggct tctggaccca
     2641 tggagggagt ggttaaaaga gcaacgagaa agtggtaatt ataatgccag ccggatatgg
     2701 cgggaaatgg tggcccaggg ggggacaggc agtgaaacca tcgtcaggga tactgttgcc
     2761 aaatggcgta aaggctggaa cccaccggtt actactgccg caagacttcc ttcagtgtcc
     2821 cgggtaagcc gctggttgat gccctggaga ataatcaggg gcgaagaaaa ttatgcatcc
     2881 cgatttatta gtctgatgtg tgaaaaagaa ccggagttga aaatagcaca gcaactggta
     2941 ctcgagttct accgtattct gaaaacctaa aataaatcac agctaagcag ctggttcact
     3001 cgagttcacg aaagcggctc agcagaactt cggtgcgtag ctgcggggat ggaagctgat
     3061 gctgcggcta tatgtgaggc aatcagcagt cgctggagta atggtgttgt cgaaggtcat
     3121 gtaaatcgcc tgaaggtgct gatacgccag atgtatggtc gagccggatt tgaactactt
     3181 aggcggaggg taatgagtcc actggcatga aaatcttcac ctaggttgcg gaagagccac
     3241 ttttgcactg ttcgtgacag tatggccttt atgtactcac tttgttaccg gtagctgaat
     3301 catcactacc gtgctgcaaa tggtggtgaa cagcatcaat tgtggaatga atgttgccga
     3361 agcgaccaat gcgcctcgtt tccaccatca gtggttgccg gactagctgc gtgttgaaaa
     3421 gggtttagtc cggatacgct caag
//
LOCUS       DAOFSV010000267         3240 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.267, whole genome shotgun sequence.
ACCESSION   DAOFSV010000267 DAOFSV010000000
VERSION     DAOFSV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.267"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(18..329)
                     /gene="traL"
                     /locus_tag="QBH27_004702"
     CDS             complement(18..329)
                     /gene="traL"
                     /locus_tag="QBH27_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDQ7016903.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWV
                     K"
     gene            complement(334..696)
                     /gene="traA"
                     /locus_tag="QBH27_004703"
     CDS             complement(334..696)
                     /gene="traA"
                     /locus_tag="QBH27_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDQ7016904.1"
                     /translation="MDAFLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            complement(730..957)
                     /gene="traY"
                     /locus_tag="QBH27_004704"
     CDS             complement(730..957)
                     /gene="traY"
                     /locus_tag="QBH27_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HDQ7016905.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            complement(1045..1722)
                     /locus_tag="QBH27_004705"
     CDS             complement(1045..1722)
                     /locus_tag="QBH27_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDQ7016906.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVFCFYAL
                     GYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEILNVLSE
                     YMGVKKM"
     gene            complement(1856..2239)
                     /locus_tag="QBH27_004706"
     CDS             complement(1856..2239)
                     /locus_tag="QBH27_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDQ7016907.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDEG"
     gene            2570..3172
                     /locus_tag="QBH27_004707"
     CDS             2570..3172
                     /locus_tag="QBH27_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDQ7016908.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
BASE COUNT         1000 a          698 c          579 g          963 t
ORIGIN      
        1 caatcctggt atcagttcta tttaacccac tgccggaaac acgaatcggg aacattatga
       61 aaaataccgc gcagcagggc tgttggcata taccagtaaa tcaggtctcg taaccaggaa
      121 ctgccccgcc cttttttcag ttttttaatc ccgaaataaa ccagaaccgc tgcaccaata
      181 ccgaacagat attttgatgt tgtgataccc cagccaatac agattgcggc ggggatcagt
      241 tcatccagtg gcaggccaaa ccagcggctc tggttggtca gtgtttccgg gaaacgatat
      301 ttcttaagtt tattctcgtc tcccgacatc gttttatttc aggccaacga cggccatacc
      361 cacagcaata aatacagaga tgatggcaaa accggccagg aacttgacgt ttttggtcat
      421 catgtacatg accgcaccga ccagaacttc agccagaaca acccatttaa caacactgga
      481 gtccttaccg aaggtcgcct taaccgtggt gttaccgctt gccatcaggt cctggccctg
      541 tgctgccatt gccagttgcg ggaagaacat catcagaaca gcagcaggga tcacagcgcg
      601 agccaggcga agcatattca gacgagtgaa tttagaaaaa aacgatttct ttttgacggg
      661 cgcagaagca ccctgaacac ttaaaaaggc gtccatatga ttcctctgtt taatattgat
      721 agataaaaac taaagttctt taaatgttga ctcgttctct tcgatcacct ccctgaatat
      781 ttcttcatta tagaaatcag gaaaccgttt cagatgatct ctgagacgga tctgaacctc
      841 tatagtttta cttctaccgc tacgatcttt ggcccgaata agtcggttat ttgtatcttc
      901 atcgagatac actgagactg ttctgcttat cccgcctctc gcgttacgcc tcctcactca
      961 attcacctct gtttcctgac caataaaatc atgattgcac agaaatggcc tgtcgaaaat
     1021 gggcccaaaa cgagccctgc tttattacat ttttttaaca cccatatatt cactcaaaac
     1081 atttaatatt tcgtacatta cacctgtctt aatacattta ttcctgaaca aatcgaagga
     1141 acccaatctt ttattgatat ccgcattaat ctttttaatc ctttttttag aagccacttc
     1201 tgtgattttt aattctttag ctatatttat atgtttatac cctaatgcat agaaacaaaa
     1261 aacaaccaat gattttgctg tcatattcga tataacagac tgtacatagc tatcacgatg
     1321 ataataattt tctttttcca cacgaaagaa tggatagtca ggaaatatat taatttgcca
     1381 tagaacaaca gattcttcat tataaaatga aacattttcc atccttattt gatagatttc
     1441 tccatgaaaa ttaaaacgac gacaaaatgc ggaaccatgc ccaagagact ggcattcaag
     1501 ctcaagtgat gaaagaaaaa tttcaacctg cagacgtaca taactttcac cagagaaggg
     1561 tttatcttct ttggaaaaga gttcaataaa ggccccattg gcataaagga ttttcctttt
     1621 tttgttacgg acacaaacag gaacaggaga attcctgtag atattttgaa cgttaagaat
     1681 gtatttattt acagattgac tgttaagtgg cctttctctc atcggcgatt tttaatatca
     1741 agttttaacg tggcattaac cacataatta atataacctt tatgcggtta attgtcatca
     1801 aattgaacca gatcaaaatc ctgttatttg aagcaaatca acttttaatt cttttttaac
     1861 cctcatcatt ttctggaaag aacctctcca tctcagatga caccttctcc ctgatatctt
     1921 cgaccatatt cgcatattca aactttggat ttccggagac atgaggactg agagactcaa
     1981 tacccaaaat tttcgctact gatgattgtg tttttacaac gcattcaaga agcaatttat
     2041 taaactcagt ctgattaaat gcagactctt tacgctccat ctgagtctca tatacacgaa
     2101 gccctaattc gagaagcata gtggatatgc ttgagaaact tacatctgta cttttagctc
     2161 cttctgcacg tcttctttcc acaattttat ttattttata gacaatttcg tcactgacat
     2221 aagcctgcac tttcgccata tgtatacctc tttattttct tatcgcatca aatttatttc
     2281 acatataaaa aaaagacaat tcatcgatga atcgaatcgt gacgcaagtt acgaaaatta
     2341 cgattcatgc agcaaatcac atcacgaatt gaatctagag tcaattcgct ttaactcgtt
     2401 gttttattat tactgattca tctatgagtt gtgttaaatt cattggtgaa tcatatgcga
     2461 ttcaccaatg aatccttttt ataatgtaaa ataaattaaa atacattatt taaaacataa
     2521 gttaatgatt caagtagcaa atcagcaaaa acttgttttt gcgtagtgtg tggtgctttt
     2581 ggtggtgaga accaccaacc tgttgagcct ttttgtggag tgggttaaat tatttacgga
     2641 taaagtcacc agaggtggaa aaatgaaaaa atggatgtta gccatctgcc tgatgtttat
     2701 aaatgagatc tgccaagcca ctgattgctt tgatcttgca ggccgggatt acaaaataga
     2761 tcctgattta ctgagagcaa tatcatggca agaatcccgt taccgggtta atgccatcgg
     2821 tattaatccg gtaacgggat atggcagcgg actgatgcag gtagattccc agcattttaa
     2881 cgaactggcc cgctacggaa ttaagccaga acatctgaca acagatccct gcatgaacat
     2941 ttataccggt gcttattatc tggcaatagc atttaaaaaa tggggcgtca cctgggaggc
     3001 cgttggtgca tacaatgccg gattcaggaa aaccgaacgc cagaaccaga gacgtcttgc
     3061 ctacgcatca gatgtttacc ggatttatac cgggataaaa agcagtaaag gtatccggct
     3121 tccggccacg aagaaatcac ttccggaaat taacagcgtg cagaacaatt aatcttacag
     3181 acattgtcag ataccggtta tgccgcaaaa gcggcatgga aggccacaca caaaaatatc
//
LOCUS       DAOFSV010000268         3157 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.268, whole genome shotgun sequence.
ACCESSION   DAOFSV010000268 DAOFSV010000000
VERSION     DAOFSV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.268"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(114..872)
                     /gene="loiP"
                     /locus_tag="QBH27_004708"
                     /gene_synonym="yggG"
     CDS             complement(114..872)
                     /gene="loiP"
                     /locus_tag="QBH27_004708"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDQ7016909.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            1150..3141
                     /gene="tkt"
                     /locus_tag="QBH27_004709"
     CDS             1150..3141
                     /gene="tkt"
                     /locus_tag="QBH27_004709"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ7016910.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
BASE COUNT          719 a          876 c          847 g          715 t
ORIGIN      
        1 ggataagatg cgccagcatc gcatccggca acgcgcacag gataacaaat gtgccattca
       61 tgtcagatgc ggcgtaaacg ccttatctga cctacgtttg gcaccaccat gccttactta
      121 actccatccg cgctcatacg atcgcgaata tgttgggcgc gttcggcgga tgctgggtgg
      181 tcgtcaaaca ttgaactttg gcgaccttct tccagtttag ccagtttttc aaagctggtg
      241 gcaaggccag ccggactgat gccgcgttgg cgcagcaaat cgtaggagta atcatcggcc
      301 tctgcttcct ggcgctggga gaattgcgaa ttgactagct tttcgcccag gtctcctagt
      361 tgtgattgtg acaaactgcc gacaattccg cctgcagagg ccgccgcaac gcgaattgcg
      421 tttgtaccaa gtgctacctg cattcctttc ttcacatggc ctaatgccac gtgccccatt
      481 tcgtgaccga gcaccgcttc gacttcgtta tccgtcatca tatccatcag cccgctatag
      541 acgcggatac agccgttagc cattgcaaag gcgttcacat ccttcgccat atacactttg
      601 taattcaccg gctgaccatt gatgttatcg cctaacgcgc gagaaattgt cgtcagacgt
      661 ttagcgtatt cgctattggc tggcgcaatc gtcgccttgc tgtccatatc ctgacatgcc
      721 tgatcgctca gggttttcac ctgcgcatca ctcaaactgt aagcctgaaa agcttccgct
      781 cctgatgaga gcagtccact ggagtccata ttctggcaac cagtcagtac cgttgccacg
      841 ctcattgcta ccaataaggc gcgaattttc atgttttctc ttccgcacaa ggtcaggatt
      901 attctaaaaa tgcgccatct gcaggtgaat tgacgctcaa tcgaagtata aggaaaagtg
      961 cagtggaccg gcgagtagat tgcgcaacat gcgagcatga tccagagatt tctgaagcgg
     1021 caaaaggatg ttccatgtac atgacgcgcg gcttgcggta aattgttggc aaattttccg
     1081 gcgtagccca aaacgcgctg tcgtcaagtc gttaagggcg tgcccttcat catccgatct
     1141 ggagtcaaaa tgtcctcacg taaagagctt gccaatgcta ttcgtgcgct gagcatggac
     1201 gcagtacaga aagccaaatc cggtcacccg ggtgccccta tgggtatggc tgacattgcc
     1261 gaagtcctgt ggcgtgattt cctgaaacac aacccgcaga atccgtcctg ggctgaccgt
     1321 gaccgcttcg tgctgtccaa cggccacggc tccatgctga tctacagcct gctgcacctc
     1381 accggttacg atctgccgat ggaagaactg aaaaacttcc gtcagctgca ctctaaaact
     1441 ccgggccacc cggaagtggg ttacaccgca ggtgtagaaa ccaccaccgg tccgctgggt
     1501 cagggtattg ccaacgcagt cggtatggcg attgcagaaa aaacgctggc ggcgcagttt
     1561 aaccgtccgg gccacgacat cgtcgaccac tacacctacg ccttcatggg cgacggctgc
     1621 atgatggaag gcatctccca cgaagtttgc tctctggcgg gtacgctgaa gctgggtaaa
     1681 ctgattgcgt tctacgatga caacggtatt tctatcgatg gtcacgttga aggctggttt
     1741 accgacgaca ccgcaatgcg tttcgaagct tacggctggc acgttattcg cgacatcgac
     1801 ggtcatgacg cggcatccat caaacgcgca gtagaagaag cgcgcgcagt gactgacaaa
     1861 ccgtccctgc tgatgtgcaa aaccatcatc ggtttcggtt ccccgaacaa agccggtacc
     1921 cacgactccc acggtgcgcc gctgggcgac gctgaaattg ccctgacccg cgaacagctg
     1981 ggctggaaat acgcgccgtt cgaaatcccg tctgaaatct atgctcagtg ggatgcgaaa
     2041 gaagcaggcc aggcgaaaga gtccgcatgg aacgagaaat tcgctgctta cgcgaaagct
     2101 tatccgcagg aagccgctga atttacccgc cgtatgaaag gcgaaatgcc gtctgacttc
     2161 gacgcgaaag cgaaagagtt cattgctaaa ctgcaggcta acccggcgaa gatcgccagc
     2221 cgtaaagcgt ctcagaatgc tatcgaagcg ttcgggccgc tgttgccgga attcctcggc
     2281 ggttctgcag acctggcgcc gtctaacctg accctgtggt ctggttctaa agcaatcaac
     2341 gaagatgctg cgggtaacta catccactac ggtgtacgtg aattcggtat gactgcgatt
     2401 gccaacggta tctccctgca cggtggtttc ctgccgtaca cctccacctt cctgatgttc
     2461 gtggaatacg cacgtaacgc cgtacgtatg gctgcgctga tgaaacagcg tcaggtgatg
     2521 gtttacaccc acgactccat cggtctgggc gaagatggcc cgactcacca gccggtagag
     2581 caggtcgctt ctctgcgcgt gaccccgaac atgtctacat ggcgtccgtg tgaccaggtt
     2641 gaatccgcgg tagcgtggaa atacggcgtt gaacgtcagg acggcccgac tgcgcttatc
     2701 ctctcccgtc agaacctggc gcagcaggaa cgtactgaag agcagctggc aaacatcgcg
     2761 cgcggtggtt atgtgctgaa agactgtgct ggtcagccgg aactgatctt catcgcgacc
     2821 ggttctgaag ttgaactggc tgtagcggca tacgaaaaat tgactgccga aggcgtgaaa
     2881 gcgcgcgtgg tttccatgcc gtctaccgac gcattcgaca agcaggacgc ggcttaccgt
     2941 gaatccgtac tgccgaaagc ggttaccgct cgtgttgcgg tggaagctgg cattgctgac
     3001 tactggtaca agtatgttgg cctgaacggt gctatcgtcg gcatgaccac cttcggtgaa
     3061 tctgctccgg cagagcagct gtttgaagag ttcggcttca ccgttgacaa cgttgttgcg
     3121 aaagcaaaag cgctgctgta atcggtaaga tgctttg
//
LOCUS       DAOFSV010000269         3155 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.269, whole genome shotgun sequence.
ACCESSION   DAOFSV010000269 DAOFSV010000000
VERSION     DAOFSV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.269"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..453)
                     /gene="soxR"
                     /locus_tag="QBH27_004710"
     CDS             complement(<1..453)
                     /gene="soxR"
                     /locus_tag="QBH27_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDQ7016911.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLED"
     gene            539..862
                     /gene="soxS"
                     /locus_tag="QBH27_004711"
     CDS             539..862
                     /gene="soxS"
                     /locus_tag="QBH27_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDQ7016912.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(865..2451)
                     /gene="pdeC"
                     /locus_tag="QBH27_004712"
     CDS             complement(865..2451)
                     /gene="pdeC"
                     /locus_tag="QBH27_004712"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDQ7016913.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIRLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLFLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2880..>3155
                     /locus_tag="QBH27_004713"
     CDS             2880..>3155
                     /locus_tag="QBH27_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDQ7016914.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMRLLGITMADITAIWHNIESVMIEEMNQTPPQWPMIL"
BASE COUNT          856 a          775 c          694 g          830 t
ORIGIN      
        1 atcttccagt aagcgtgcgc cggtaccttc ttctcctaag cggtcgcccg ggttacgcaa
       61 cgggcaatca ctgcgcgaaa ggcagccaca accaatacat ccatccagtt cgtcacgcag
      121 cgccactaag gtatgaatgc gccgatccaa ctcttctcgc cattgggacg aaagctgttt
      181 ccactctttc gcacttaacg tatgcccttc tggcaacacg ccaaacgctt cacgaatggt
      241 tgcgagcgga atgccaatac gctgagcaat tttgataatt gcgacatatc gcaacacatc
      301 acgtttatat cgccgctgat tgccgctatt acggatactg gtaatcaacc ctttgctttc
      361 atagaaatgc agcgccgata ccgccacacc gctgcgtttc gccacttcgc cgggtgttag
      421 cagcgcttta atgcggggta atttcttttc cataaatcgc tttacctcaa gttaacttga
      481 ggaattatac tccccaacag atgaattaac gaactgaaca ctgaaaagag gcagatttat
      541 gtcccatcag aaaattattc aggatcttat cgcatggatt gacgagcata ttgaccagcc
      601 gcttaacatt gatgtcgtcg caaaaaaatc tggctattca aagtggtact tgcaacggat
      661 gttccgcacg gtgacgcatc agacgcttgg cgattacatt cgccagcgcc gtctgttact
      721 ggccgccgtt gagttgcgca ccaccgagcg tccgattttt gatatcgcaa tggacctggg
      781 ttatgtctct cagcagacct tctcccgcgt tttccgtcgg cagtttgatc gcactcccag
      841 cgattatcgc caccgcctgt agttttattg cccgcgcgtt aattcccgcg cgggtaattg
      901 ctccatccat tgcataaaca cctgcggcgg catcgccttc gcgaagaacc atccctggca
      961 atagcgcacg ccgcgtttgc gcagccagtt aacctgcgcc tcagtttcga caccttcagc
     1021 aatcgttttt aaccccaggc tgtgcgccag ctcgatgatg tgttccgcaa tcaaatgact
     1081 ggttttgtgg gtggtcagcg tctcaacaaa cgatttatcg attttcaaaa tatcgacatt
     1141 caatgattta aggttatgca agttagagta gccaatacca aaatcatcaa ttgccacttc
     1201 gtaaccggcc ttgcggaaag ccagaataat cggcgtcatt ttgtcgacat caagaaatgc
     1261 atgttcagtc acttcaaatt taatttgctg cgggcgcacc gcatgttgct ctgttttctg
     1321 attggttcgc gctatcaacc gtgaggtatg aaaatcggag gctgacaggt taatagaaac
     1381 atagcgatcg gcatgtgttg ccagatacgc gcccaaatcg cggaagacat tatcaataac
     1441 ataatcagtt acctgttcga tcatcccctc cttttctgcc agcggaataa actctgccgg
     1501 attcattact tgcccctgct caccgggcca acgtaacaac gcttcagcgc cgatacattt
     1561 ttctgttttg atatcgatta ttggctggta ataaaggaaa agttgatgtt tttcgagggc
     1621 gcgttgcaat ttgcgtttgg gagacaaata gttttgtcga atacgtagcc agagtaatag
     1681 cagaacaaga ctccccaaaa taccggcggg caacgcaaaa ataagatgat tataaaaatg
     1741 ggttataaga cgttgatatg aagtcgcaac aatggccgca attgggcgtt ttgtcgaata
     1801 aactgtcgca tataaatagc catttcgttg tacggttaaa tcattcaaac gaatcagcgg
     1861 agaaaacgtt gctgccgagg cctcgttgct taacgagaaa aaggttttcg tcaccgtatc
     1921 atacacaccc cattgcaatg tcggatcatc agacatcact tcactccaga agagagggtt
     1981 aatgaccgct acataattcc cccgctgcat ataggtcatt ttatagcctg agaaaaaagg
     2041 cgtatcacgg taataataga tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat
     2101 cgtatagcca tttacagacg ctatcagcga tgagcataaa aaatggttat cacgagcata
     2161 aatcaattca ttaatataaa gatagccacg aataatattc aacattcgct tttgatgggc
     2221 tggagtgcaa acttgcccct gatagcgttc agctgcatcg ctcactaaat ctgcctgacg
     2281 aatgaccagc tcagatttat ccaacgccag ctgagcaaaa gttcggagct gattattcac
     2341 ttctgatttt gcccaaaaga aggcaatcca tagcgacaga atgatgggaa agagaactaa
     2401 aaagataatg cccggaaacg ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa
     2461 ttccctgcct gtttataccc ggttattgca aggtgaaacc ctggctaccg acgtctttag
     2521 ataaatctta cggtataaat agcacttttg cctgatataa aattgagacc tttttaatcg
     2581 tcaccaggat gacgataatt cattgatctt aaaaattaat caccttcagg aatgcagagg
     2641 cggcgggaag atataccctt aatgccgctg aaaaaacaca taatctgtac ggcaataaaa
     2701 gtcacggcct aatctggcta acaaacaacc aattgtgata tagttcacaa aattcatgaa
     2761 acaaacagag tgtttcattt ttgtgttctg caagccccgc gatagccaga gtgtatcacg
     2821 cctcccgtga acaacgccgc gctgtccagg gcatcagctc tttttcgagg aattgtttga
     2881 tggctaccct caccactggc gtggttcttc ttcgctggca acttcttagt gccgtaatga
     2941 tgtttctggc cagcacgctc aacatccgtt ttcgtcgggc tgattatgtc gggcttgcag
     3001 tgatcagcag cggtttgggc gtagtttccg cctgctggtt cgcaatgagg ttgcttggca
     3061 tcacaatggc ggatatcacc gccatctggc acaacatcga gtcggtgatg atagaagaga
     3121 tgaatcagac accgccacaa tggccaatga ttttg
//
LOCUS       DAOFSV010000270         3129 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.270, whole genome shotgun sequence.
ACCESSION   DAOFSV010000270 DAOFSV010000000
VERSION     DAOFSV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.270"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(1338..1673)
                     /locus_tag="QBH27_004714"
     CDS             complement(1338..1673)
                     /locus_tag="QBH27_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDQ7016915.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFTDTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1802..2149
                     /locus_tag="QBH27_004715"
     CDS             1802..2149
                     /locus_tag="QBH27_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016916.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(2169..2678)
                     /locus_tag="QBH27_004716"
     CDS             complement(2169..2678)
                     /locus_tag="QBH27_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016917.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLPAGGTSATDLAV
                     ELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGMEVR
                     VQINESAAP"
     gene            complement(2675..2935)
                     /locus_tag="QBH27_004717"
     CDS             complement(2675..2935)
                     /locus_tag="QBH27_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016918.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          899 a          737 c          632 g          861 t
ORIGIN      
        1 accctgtaaa acgccctcaa tcgctttctg gcgcaaaaaa cgcaaaaaga caagaaaaca
       61 ccaggctaaa ccagaaaaac gcgccagaat cgtttttaga tggctgcata actatgcacg
      121 aaagcgaccc gcgatcggtt ttgtgtgttc tgaataccga acgggtcaaa atgacacgtt
      181 ttctctccct caatggagcg ttctatacag agatttttgt tcccctatcc attattctga
      241 ttaccgggtt tccaagctga aaacaggact tttctgcgac cagtttttgc gaatatagtc
      301 acataaacca ccgattgagt gacaaataca ggagaattta ttttgaaata tgattttatc
      361 aatggcagtt ctattttaat tatagtctgg atgactatag ctgtaataat cagagtcaca
      421 aactggaaat tcaacaaaaa aacagcccca gctataagct tattcttagc tttccctgca
      481 tctatagtta ttctgttagt ttatattttt ttcttcgata agtgatgtgt tttcatgatt
      541 aatcaagttt ctgttcaaat gctcctgctg gcccagcatg gaaaaagaaa ttacgaataa
      601 atgcacccag tgccattaac atctatctac acaaaaatag aggaaaacaa ttttgatata
      661 tcaaataatt acaagcagct ccattctttt tattgtttgg gggattacat ctataataat
      721 tagaacaaag aattattttt ctaagaagaa aacagacctt aatataagct tttttatagc
      781 cttccctatt tctataacaa tccttttatt ttgccgaggt atattataaa tataacataa
      841 tacatctata tggggtgtac atatccccac ataaaaacac atactctcat taacacggga
      901 agaaactatg ttttccaatt ctcactacat gtacataatc attcttgtac tatggtttag
      961 tattctcaag tacttacaga gaaagaatag catattacat cctttatttt cactaatcct
     1021 gacaccacta accattttaa tcattagact tatgatagat ttaacataca accaataaat
     1081 tataaacaaa ataaaaatag ggaacattat atgctttata aaattttcac attaaacaca
     1141 aagataaaaa caactatcat tacaatatgt gcattatcat ataatcttac agcgcaagct
     1201 tccccgtcat acagacctcg tgatcgagtt aataagacta tgatagaact acaaactaac
     1261 ggagtagaac ggcaactttt agataattct tgtggcataa gttctggggt atttaaataa
     1321 aatataagac aggctgtcta tcttacagac agccttttta taataacaga aagaatatat
     1381 atcatactta acggaagaga taatatgaaa acagtacccg tataaacagc aagcaatccc
     1441 atttttccag gtgtatcagt aaaaaaaccg ctgttccaga aatcaggccg ggtaaatttc
     1501 agagctgtat cttcaatata ccattttgca accggataca aaaccattcc gcagagagaa
     1561 atacaccaga acagtaatct gtatttaaaa tcataatccc atgacatata cagcatatat
     1621 ccccccgtca cccatcccca ccacatatta ttaaaataat attttctgtt catctgccag
     1681 ttttcctttc ttttgcactt ttgtcagtgt actgatgcat gacaacataa ccacagcatg
     1741 ataataataa tcaataacaa taagctgtgt cacgtttaca tcatggaacg gaggtgctcc
     1801 tgtgactttt gagcagaaaa aagcccgggc cattgccctg atggacagca aaaagatgtg
     1861 gcggagcaat tatgcccctc ctcttctgcg tattctctgg cggctgggta tccggcttcc
     1921 gccattaccc tttatgccgt tctggcaggt cacggtgctg acggggggct tatggggcat
     1981 ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg tcaggaatgg tggcaggtga
     2041 ggccattatt ataagtatca cgggggggtt tttgtccgga ctgctcatgg cctctttcca
     2101 ctggtggcgc aggaaagtta accggctgcc accatgggat gatgtgtaaa ccgtgcacag
     2161 gcgtgttctt acggagcggc actttcattt atctgtacgc gaacctccat tcctgcatcg
     2221 tgacaggcct gcagccactg cgccacttcc agcggatcgc cctcccggcg taccaccctg
     2281 ccttctttat tccataactg cagacaggtg ctgccgtcga gacgcaccac aaaatcccca
     2341 cggcaggcct gatacgttat gccgttcagc tccaccgcca gatcagtggc agaagtgccg
     2401 cctgcgggca gcagaggctg tgccacggcc cggccggcgt ccgtcagctc cactgccagc
     2461 tgcaggttcg gagcgcgcag ggtacgcagc cagccggcat cctccagacg acggcaggag
     2521 gcgcgcaggt ttggcccgta gaccggggct tccccgcttt gctccagcac ccgttctatg
     2581 tcccgcgtca gcacgggtcc tggccgtttt tcatcaaggg cagccagcac aatcagcact
     2641 ctccgctgca gtggcgacgg tcggcggctc tgagtcattt ccagtcatcc tccggccaga
     2701 tgagctttct gaggcgctga tggtcggcgt caatgatctc ctgtctcttt atggcattca
     2761 ttgaggtata gccatttatg gccagttcat gccagaagat cagcacggct ccggctttgg
     2821 ctgactcgca gagataagtt acgccgtggc tgtccagatt gcattcccgg atgcgttttt
     2881 ccgcaaaccg ggtcagttgt tcatagctga gtgaaaacgt catttcctga tccatcctgt
     2941 acgttccatc tgataatgtt ctgacaggta aaaatcatat cctgaaccgc cccgggaatc
     3001 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac tcgtttttcc
     3061 cccgaagtcc gtcagagggc gattcgtatg gttctggaaa gtcagggcga atatgactca
     3121 cagtgggcg
//
LOCUS       DAOFSV010000271         3128 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.271, whole genome shotgun sequence.
ACCESSION   DAOFSV010000271 DAOFSV010000000
VERSION     DAOFSV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.271"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..2094
                     /gene="sinH"
                     /locus_tag="QBH27_004718"
     CDS             <1..2094
                     /gene="sinH"
                     /locus_tag="QBH27_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HDQ7016919.1"
                     /translation="NESLPDLGSQAAQQDEQTNKGKSLKERGADYVINSATQGFENLT
                     PEALESQARSYLQSQITSTAQSYIEDTLSPYGKVRSNLSIGQGGDLDGSSIDYFVPWY
                     DNQTTVYFSQFSAQRKEDRTIGNIGLGVRHNFDKWLLGGNIFYDYDFTRGHRRLGIGA
                     EAWTDYLKFSGNYYHPLSDWKDSEDFDFYEERPASGWDIRAEAWLPAYPQLGGKIVFE
                     HYYGDEVALFGTDNLEKDPFAVTLGVKYQPVPLIAVGTDFKAGTGDNTDTTVNATLNY
                     QFGVPLKDQLDPDKVSAAHSLMGSRHDFVERNNFIVLEYKEKDPLDVTLWLKADAMNE
                     HPECVIKDTPEEAIGLEKCKWTINALVNHHYKIVAASWQAKNNAARTLVMPVIKENTL
                     TEGNNNHWNLVLPVWQYSSDKAEQGKLNTWRVRLALKDEKGNRQNSGVVEITVQQDRK
                     IELIVNNIANVPEENNHSHEASAQADGVDGVVMDLDITDSFGDNTDRNGNVLPQDNLN
                     PQLFDANDKKVTLTNNPCTTETPCVFIAKQDKEKGTVTLSSTLPGTFRWKAKAAPYDD
                     SNYVDVTFLGADIGGLNAFIYRVGAAKSVNLIGNKEPLPLNSRYRFVLWRDANKDGVF
                     QLSEKLTDEEMKQYDYQWEFTGHSVNGNTGAQANTTNVDIEIPATNKEAAAKFSAQVT
                     DGVQGYGLQVNYSKT"
     gene            2150..>3128
                     /locus_tag="QBH27_004719"
     CDS             2150..>3128
                     /locus_tag="QBH27_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016920.1"
                     /translation="MKPHMKRGLTKVALALMLAGYCAVPVAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSEDADHIKVTSDSKGIRPAGSESDKRLYSGDTITLGWDIGDT
                     EGDIDDGSAGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKITDGDRGRYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKDTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRNGKTGLAIPANGDGVQGYTLSII"
BASE COUNT          874 a          741 c          810 g          703 t
ORIGIN      
        1 aacgaatcgt tacctgattt aggtagccag gcagcacaac aggatgaaca aaccaacaag
       61 ggtaaatcgc tgaaagagcg cggagccgat tacgtcatca actccgccac gcaagggttt
      121 gaaaacttga cccctgaggc gctggaatct caggccagaa gctatctgca aagtcaaatc
      181 acctcaaccg cacaatctta tattgaagac acactctctc cctacggtaa ggtccgttcg
      241 aacctctcca ttggtcaggg cggcgatctg gatggcagtt ccatcgatta ttttgttccc
      301 tggtacgata atcaaaccac tgtttatttc agccaatttt ctgcgcaacg aaaagaagat
      361 cgtacgatcg gaaatatagg gcttggtgta agacataatt ttgataaatg gttattgggt
      421 ggaaatatat tttatgatta tgattttacc cgtggacatc gccgtttagg tataggcgct
      481 gaagcgtgga ctgattattt aaaattttct ggtaactatt atcatccgct ttctgactgg
      541 aaagactctg aagatttcga cttttatgaa gaacgccctg cgagcggttg ggatattcgt
      601 gccgaagcct ggcttcccgc ttatccgcag cttggcggta aaatcgtttt cgaacattat
      661 tacggggatg aagttgcgct atttggtacc gacaatctag agaaagatcc cttcgctgtc
      721 acgttaggcg tgaaatacca gcctgttccg ctcattgctg taggaacgga cttcaaagca
      781 ggaaccggtg ataacaccga taccacggtc aacgcaacgc ttaactacca gtttggcgtc
      841 ccgctcaaag accagcttga tcctgacaaa gtgagcgcgg ctcactcact catgggcagc
      901 cgtcacgact ttgttgaacg taacaacttt attgttctgg aatataaaga gaaagatccc
      961 ctcgatgtca ctttgtggct gaaagctgat gcgatgaatg aacatcccga gtgcgtgatt
     1021 aaagatacgc cagaagaagc tatcgggctg gaaaaatgta aatggaccat taacgcgctt
     1081 gtcaaccacc actacaaaat tgtcgctgcg tcatggcagg cgaaaaacaa tgccgcccgc
     1141 acgctggtga tgccggttat caaagagaat accctgacag agggcaacaa taaccactgg
     1201 aacctggtgc tgcccgtctg gcagtacagc tctgacaaag ccgaacaagg aaaactcaat
     1261 acctggcggg ttcgcctggc gctgaaagat gaaaagggca accgacagaa ctcaggcgtg
     1321 gtggaaatca ccgttcagca ggaccgtaaa attgagctga ttgttaataa catcgcgaac
     1381 gtgccggaag agaacaacca cagccacgaa gccagtgcac aggctgatgg tgtagatggt
     1441 gtggtgatgg atctcgatat tactgatagc tttggcgaca acactgaccg caacggtaat
     1501 gtcttgccgc aggataatct caacccgcag ctgtttgatg ccaacgataa aaaagtgacg
     1561 ctgacgaata acccctgcac taccgagact ccctgtgtct ttatcgccaa acaagataaa
     1621 gaaaagggga ccgttacgct ctccagtacg ttgcctggca ccttccgttg gaaggcgaaa
     1681 gctgccccct atgatgacag taactatgtt gacgtaacgt tcctgggggc tgacatcggc
     1741 gggctgaatg cctttatcta tcgcgttggt gcggctaaat ccgtgaatct tatcggcaat
     1801 aaagaaccgt tgcccctcaa tagccgatat cgctttgtgt tgtggcgcga cgctaacaaa
     1861 gacggtgtgt tccagctatc tgaaaagctc actgacgaag agatgaaaca gtatgactac
     1921 caatgggaat tcacggggca tagcgtaaat ggcaatactg gcgcgcaagc caataccact
     1981 aacgtcgata tcgagatccc ggcaacgaac aaggaagcgg cagcgaagtt tagtgcccag
     2041 gtaaccgatg gtgtgcaagg gtacggtttg caggtcaatt acagcaagac ataggaacag
     2101 cgccatggct gatcgggcca ccgctgaatg ttaaccaagg agagtgttta tgaaacctca
     2161 tatgaaacgt ggcctgacaa aagtcgcgct ggcactgatg ctggctggat actgtgccgt
     2221 accagtcgct atggcggaag atgctgcctg ggtcgccagt ggaacgacag ctgaattcga
     2281 ggggaccata ccctggctct atcgcgaagg ggggaatgct acgattaact cggaagatgc
     2341 ggatcacatc aaagtgacat ctgatagtaa aggtatccgt cctgcaggta gcgagtctga
     2401 taaacgcctt tactcagggg ataccatcac gctgggttgg gatatcggcg ataccgaagg
     2461 ggatatcgat gatggttccg caggcattga tgcgaaaacg acggcgacca tcaaatggta
     2521 cagctacagt gataatgcgg gtggcggtaa aacggagttg actgcggcgg caggaaaaac
     2581 cagttataaa atcaccgatg gcgatcgtgg acgctatatc ggtgttgaaa tccagcctat
     2641 cactcagacc ggtaatccct tccagggaac ttcgctgact ctgctggata tttctacggc
     2701 cagcggtggc ggcagcgata cggataatgt cgatcctggt ccggttgtta accagaacct
     2761 gaaagttgcc atcttcgaga aagacaccag taccaacctt atcggtggta atacagccat
     2821 tgcgcttaac aaaacgtatg tggccaaact gtactcggat gaaaaccaga acggtaaata
     2881 cgacgcgggt acggatgtgg acgttaccgc taactacgac tttgcgtggg tctttaacgg
     2941 taacagtaaa caacttgcgg cggcgggcgg tattgccaac gccagcttcg ataacaatga
     3001 cattgttatt ccgcaaacta acgaacaagc aagaaccagc cttaacggta gtgaccgtaa
     3061 tggtaagacc ggccttgcaa tcccggcaaa cggcgacggc gttcagggct atacgctgtc
     3121 tatcattt
//
LOCUS       DAOFSV010000272         3125 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.272, whole genome shotgun sequence.
ACCESSION   DAOFSV010000272 DAOFSV010000000
VERSION     DAOFSV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.272"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="QBH27_004720"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="QBH27_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDQ7016921.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="QBH27_004721"
     CDS             complement(643..882)
                     /locus_tag="QBH27_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDQ7016922.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="QBH27_004722"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="QBH27_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDQ7016923.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2335..3000)
                     /gene="yecA"
                     /locus_tag="QBH27_004723"
     CDS             complement(2335..3000)
                     /gene="yecA"
                     /locus_tag="QBH27_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDQ7016924.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT          734 a          769 c          740 g          882 t
ORIGIN      
        1 attcgtattt ttaataaatg atgtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gctacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaacttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac gcagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcaa tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagtgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggttttctgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gttgaaaaac cttcttctga aggtataaat ttgccttttg ctgccagaaa
      721 cgccaccaac tctcccgcag tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaattg ctgcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgctg cttatgaaga ctttgcgcca gcgcagaact gaacgctttt
      961 tattgtacat ttatatttac actatatgta acgtcgattt gacgaatcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaactctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggcag gtgttggttt tagcgtcacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtatcagc atgtcccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc agtggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgccgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gtcggaactt cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac agggactggc
     1621 actgtctgca atcccggtga tttttacgtc gtttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acattcgtaa gctacgctgg gtgtttataa tcggtagtgc
     1741 gatccccctg gtggcatata ttttctggca gctggcgacc cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc cggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtcc ttcctcggcg tagcgttagg cttatttgat tatctggcag atttgtttca
     1981 gcgttcaaat accgttggtg gacgattgca aactggtgca attacctttc tgccgccact
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 actggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca ggaagcacaa
     2161 tcctcaggcg ggttaccggg tgaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca ggcttgttac cagaagtggg
     2281 gtgattagat tcttattggg tgccagaatt aacgctgaca cccaatttga cctcttaatg
     2341 caggcagcac tgcttaaatt tcttaccact accgcacggg caaggatcat tacgccccgg
     2401 tttctcttct gctttgatcg gttgctgaac agctttttcc tgcggatgcg ccatccagta
     2461 cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg ctctcttcaa acgcttctgg
     2521 cgacatcttc tctacccgct cgaagttttc ctcagtaccg tgcagcgcaa tcgcctccag
     2581 cgctggtttt aacgaatcgg gcaacgttga ccagtcagaa agtgccacgc cccgcatata
     2641 gccaaagcac cactcctcaa caatcgtcag ctcgctgcca tcgacttctc gcaagccgaa
     2701 taatggctca aactgctccg gaaattcgtt cagacgctct gcggtatcgg ccatatgttg
     2761 aaaagccagg ttcataaagc gcgtcatctc tttctctgac gcccagcgcg gcacatagtc
     2821 agccccaccc cacacggcaa ccagccactg tgccggttca atctcttgcg gagaactcaa
     2881 caccgccgtc aataaaccgt ccagctccgc cacatcaaga atggcgtggt cagtgttgta
     2941 tttggtcaga atatcgtcca gccattccaa ctcactttcg tttaacggtc ccgttttcat
     3001 acgcttttcc ttgtggatct caactcgcca gcacctatct tacatgccgg tccgtatcag
     3061 agatactttt tgagtggctt tgccggtgat taaaaattaa ggagggtgta acgacaagtt
     3121 gcagg
//
LOCUS       DAOFSV010000273         3086 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.273, whole genome shotgun sequence.
ACCESSION   DAOFSV010000273 DAOFSV010000000
VERSION     DAOFSV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.273"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(12..260)
                     /locus_tag="QBH27_004724"
     CDS             complement(12..260)
                     /locus_tag="QBH27_004724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016925.1"
                     /translation="MSRDPPQRRRARARNGVAPYIPAAFCRDAGSAGHGATRVARPER
                     SLQARGFHASDRSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            774..1004
                     /locus_tag="QBH27_004725"
     CDS             774..1004
                     /locus_tag="QBH27_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016926.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1056..2417
                     /locus_tag="QBH27_004726"
     CDS             1056..2417
                     /locus_tag="QBH27_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDQ7016927.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            2465..3028
                     /locus_tag="QBH27_004727"
     CDS             2465..3028
                     /locus_tag="QBH27_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ7016928.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
BASE COUNT          716 a          872 c          912 g          586 t
ORIGIN      
        1 gcatccggtc gtcaggcccg actccgacag gcagctgctg gcgcacccgc cagtctccgc
       61 cgaacccgct ggcgcggttt cgggctgtcg ctacggtccg atgcgtgaaa accccgggct
      121 tgcagcgacc gttccggtcg cgccaccctt gtcgcgccgt ggcctgcgct cccggcatcc
      181 cggcaaaagg ccgccgggat gtaaggggca acgccgtttc gcgcacgcgc tctccgtcgt
      241 tgcggcgggt ccctgctcat tgcctgccgg tgccacacac ccgtatgagt ccctgtcgcc
      301 tggggctgat acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca
      361 aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag
      421 gccgtggcag tgatgcccct tgcggcgaag gttctcaaac gggtgaagtt cataccacag
      481 ggtatacagc agcaggttcc cgcgaaacgt caaaaccgcg gctcccgaaa ctcaacgggt
      541 gatgttcttt aaaaatcagg atttatttca ggctccggca atacgcattt atgtgttctg
      601 tcagtcatcc ggctggcagg taatgagaac gcaggaacag aaggaggaaa acaccggaga
      661 acgtcagacg cagatatgaa aaaagcaggt cagggacctg cttaatccac accgcagaga
      721 ctgcacgaac agtctttacg gtttcattaa ttaacgtcat aagaggctaa attatgacag
      781 taagtattgt ttcaccttcc gcagcggctg taaagccccg ccgtcatccc cgtttcagac
      841 gcgaagacat ccccgcgccg gaaattgacc ccgtgctgaa agccttcggg cgacacattg
      901 cccggagttt tcaccggggg cgtggtgtac acatccccgc catgaaaaac acggccttcg
      961 ggcaggtgct ccgcacactc gaactgaaac gcgcttttaa ctgatgaggt gagccgcccc
     1021 gcgaggggcg gcaacagacg gaaggagaac attttatgac attagcagca atcaccatga
     1081 ccgccccgga agccgccagc cctgtgcaga tgtaccgcgc gacctactca ccggatgaca
     1141 acaaactgcg cctgtatgcc gtgtcacgtc ttgacccgga gacgtataaa aaagtgcatg
     1201 atgccggttt ccgctgggca ccaaaacagg cgctgtttgt cgcgccagcc tggacaccgg
     1261 gccgggaaga cgtgctcctc tcacttgccg gagagattga ggatgaagac agcacgctcg
     1321 ctgaacgtca ggaagcacgg gcggagcggt ttaccggata cagcggaaag cgggccagtg
     1381 aatccgcaca ggcacttgat gaagtggaaa gactggccgc gatgatcccg cccggtcagc
     1441 ccattcttgt ggggcatcac agcgaacgcc gcgcccgtcg tgatgcgcag cgtattgaaa
     1501 acggcatgaa acgagccgtg atgctctttg aacgtgcgga atactgggaa gagcgggcgc
     1561 ggtcggcact gcttcacgcg aagtataaag aacgtccgga cgttcgctgg cgtcgtatca
     1621 aaaaaatcga agctgatttg cgcaaggctg aaaagaccat cgcgcagtcg cagaaatatc
     1681 tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc aaaactcatc agcagtcatg
     1741 accatatcag cgcctgtttc ccgctggata cgtatccgcg cccggcagaa aaaagccagt
     1801 atgaaggaag tcgatcgtta tggtcggccc tggatgatga catcatcacc acggagcagg
     1861 cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca gcatcaacaa cgctgggtta
     1921 accactatca gaaccgcctg atctatgagc gtgccatgct ggacgaaagc ggcggcgtgg
     1981 ttacccggac acaggatttt gagccgggcg gacaggtttt cagccggggc gagtggctga
     2041 ccatcatccg tgtgaacaaa agcaacgggg cggtgagttc agtcacaacg ccaaattaca
     2101 gttttctcgg gtacagcggc acgatgaaag tgacgcccga tcgcatcacg gactacaaag
     2161 caccatcggc agaagaggct gccgtcgcca gtcaggccgc gaagcgtccg ccggtagtca
     2221 actatccggg ggaaggtttc cgggaaatga caaaggcaca gtgggccgcc ctgccccggg
     2281 actgtaaggc cgtgcgcagt gtggcagaag cagaagacca cggggcatac cggtaccgcc
     2341 gcacaatgga caataatttc cgcctggtga atgtgtatat ctccgacatg aaaatcacgg
     2401 aaatcccaca gaaataaggt atatcccccc gggaaaatcc ccgggggatg acggagataa
     2461 tgatatgcac agtcagttaa gagaacgtat ccggctgatg cgcgcaaggc tggataacgc
     2521 cgcgccggtt gctgaaatcc gggctgaatc tcagcttttt gtgactcctg ccccggtctg
     2581 tgatcgcctg gtgatgctgg cggagatcag caaccgtgat cacattctgg aaccctctgc
     2641 cggcaccggg gccatactgc gggcgattcg ggataccgcg ccgggggcca tgtgtgatgc
     2701 ggtggaaatc aacagcgggc tggtccggta tctgcgggaa aattttaacg gtgtcagggt
     2761 tcagtgtggc gacttcatgg aatggctgcc ggtgcagtat tacagccggg ttatcatgaa
     2821 tccgccgttc agccacgggc aggatatccg gcatatcctg cgggcctttt ccctgttgcg
     2881 tccgggtggc gtgctggtcg ccgtctgtct caacggacca cgccagcagg agaagctgtt
     2941 accgttttct gacgtccgcg aggagctgcc gcgcggcacg tttgcttata ccgatgtccc
     3001 gacgatgatt attcgtctgc gtgcctgatg acacagggcc ggcgggagga taaatgcctg
     3061 tctgcccgcg ccgggaaaaa acgcgc
//
LOCUS       DAOFSV010000274         3085 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.274, whole genome shotgun sequence.
ACCESSION   DAOFSV010000274 DAOFSV010000000
VERSION     DAOFSV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.274"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(4..954)
                     /gene="corA"
                     /locus_tag="QBH27_004728"
     CDS             complement(4..954)
                     /gene="corA"
                     /locus_tag="QBH27_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDQ7016929.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1324..2088
                     /locus_tag="QBH27_004729"
     CDS             1324..2088
                     /locus_tag="QBH27_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDQ7016930.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            2171..3073
                     /locus_tag="QBH27_004730"
     CDS             2171..3073
                     /locus_tag="QBH27_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDQ7016931.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
BASE COUNT          808 a          728 c          796 g          753 t
ORIGIN      
        1 tttttacagc cagttcttcc gcttaaagta cagatacggt gccaggcccg cgaggatcat
       61 aaagataatc gcgcctgggt agccgaagct ccacttcagt tccggcataa actcaaagtt
      121 catcccgtag ctggaagcga ccagcgtcgg tggcaggaac accacggata ccaccgagaa
      181 gattttgatg atgcggttct gctcgatatt gataaagccc atcgccgcct gcatcaggaa
      241 gttcaccttc tggaacaggg attcgttatg cggcagcagg gattcgatat ctcgcaggat
      301 ttcacgcgcc tgctccagtt gcccacccgg taagcgtgct ttacgtacca ggaagttgag
      361 cgcgcgctgg gtatccatca gacacaggcg aactttccag ccgatatctt ccagttccgc
      421 cagcgtggag agcgcctcgt cgtactcatc gccctgatgc ccttccataa ttacccggct
      481 caactgctcc aggtcgctgt agatattttc aatttcatct gccaactgtt cgattttggt
      541 ttcgaacaga tccagcagca gctcgtaggc gttaccgtcc accattgact ggctacgggc
      601 acgcatacga tacagacgaa aagcgggcag ttcacgctca cgcagagtaa acagacgacc
      661 atcacggatg gtaaatgcca cagtggagtt accggcgtga tcttccgcat cttcaaagaa
      721 gaagaaggag tgaatatgca ggccgtcatc gtcttcaaag aaacgtgccg atgcttcgat
      781 gtcttccagt tccgggcggg ttgccaggct ctggccaagt tcagattgta cgcgcagtcg
      841 ctcgtcgtcg tccggttcga caagatcaat ccatactgca tttacaaggg gttgtgactc
      901 ttcgacttcc agccgggtca gtcggttatt ttccagttga aatgcgctca gcatgaccgg
      961 gactcccaat gcttaaaata tcggacagtt cggtgggcac acagaaacaa attgggtttc
     1021 agaccattaa acagcctgac tcagcgcgac gggaaaaatt gaggtcgctg acaaccgcta
     1081 aggctatcag caaaaaggga tagccttagg agttgatcct ggatgacagg atagtgagcc
     1141 agtatctact gggtgtgtcc aaggcgaatg tcctcttaga gtgatcgtgc gcgcatgtta
     1201 cgccagcaaa attttgccgt caacacgcaa cagaacacca cagaacaata gttgaacttc
     1261 gcgctttttg gtactcgtca aggtcgctaa caggataaaa aaatcccaca ttcagggcct
     1321 ataatggcgc atcggctatt cattggtaaa ggaatgatca ccttgaatct caaacggatt
     1381 tttctcaccc tcaccttact tcccttgttt gctgttgccg ctgatgattg cgcaccctca
     1441 gatccgacgc tgaccgtaca ggcgtatacc gttaatcctc aaacagagcg ggtgaaaatg
     1501 tactggcaaa aagccaatgg cgaagcgtgg ggaacgttac atgcgctgct ggcggatatt
     1561 aatagtcagg gtcaggtgca gatggcgatg aacggcggca tctatgatga aagctatgcg
     1621 ccgctcggtt tgtacatcga aaacggtcag cagaaggtgg cgttaaatct cgcttcaggt
     1681 gaagggaatt tctttattcg ccctggcggc gtgttttatg tcgcgggaga taaagtcggc
     1741 atcgttcgtc tggatgcctt taaaaccagt aaagagattc agtttgcggt gcagtcaggg
     1801 ccattgttga tggaaaacgg tgtaattaat ccgcgtattc atcccaacgt cgcctcacgt
     1861 aaaattcgta acggtgttgg gattaataaa catgggaacg ccgtgttttt gctgagccag
     1921 caggcgacaa atttttatga ttttgcctgt tatgccaaag cgaaactgaa cgttgagcag
     1981 ctgctttatc ttgatggcac aatttcacat atgtatatga aaggcgaggc aatcccgtgg
     2041 caacgctatc cttttgtcac catgatttcc gtcgagcaga aaggttaaac attgtacact
     2101 cgaggaggcc agacaaactg gggctggcta cacagctgga aaatcatcag ataaatgaag
     2161 ggaaacaaaa atgaaaaaaa cgctcgcaac attattttta ttaacctgtc tggggtcatc
     2221 atccgcttat gcggataacg ctttgatttt acaaacggat tttagcctga aagatggtgc
     2281 cgtctcggcg atgaaagggg tcgcattcgg tgttgaccat aatcttaaaa tctttgattt
     2341 aacacacgaa attccaccgt ataacatctg ggaaggcgct tatcgcctgt atcagactgc
     2401 cagttactgg ccgcaaggtt cggtgtttgt tagcgtggtc gatccaggtg ttggtacaga
     2461 ccgtaaatcg gtagtgctga aaaccaaaaa cggtcagtac ttcgtttcac cagataacgg
     2521 tacgctcacg ttggtggcgg aatcgctggg tattgagagc gtccggcaaa tcgatgaaaa
     2581 aaccaaccgt ctgaaaggtt ccgaaaagtc atataccttc catggtcgtg atgtatatgc
     2641 ctacaccggt gcgcgtctgg cttccggggc gatcacgttc gagcaggtgg ggccagagct
     2701 tcctgcaaaa gttgtcgaac tttcttatca gaaagccaaa gcaacaaaag gtgaagtgaa
     2761 gggcaatatc ccgattctcg atattcagta cggcaacgtc tggagcaata tcagcgacga
     2821 gttgctgaat caggcaggaa tcaaactcaa cgacacgttg tgtgtgacca tttccgaagg
     2881 ttcccagcaa aaatacgcag ggaaaatgcc gtatgtcgca agcttcggcg atgtgccgga
     2941 aggccagccg atggtgtatc tgaacagctt attgaatgtt tccgtggcgc tgaatatgga
     3001 taatttcgcg cagaaacatc aggttgcgtc tggtgctgac tggaatattg atgtgaagaa
     3061 gtgcgctaag taaggtgctg atgcc
//
LOCUS       DAOFSV010000275         3043 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.275, whole genome shotgun sequence.
ACCESSION   DAOFSV010000275 DAOFSV010000000
VERSION     DAOFSV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.275"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(48..905)
                     /locus_tag="QBH27_004731"
     CDS             complement(48..905)
                     /locus_tag="QBH27_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7016932.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            1153..2022
                     /locus_tag="QBH27_004732"
     CDS             1153..2022
                     /locus_tag="QBH27_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDQ7016933.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(2182..2775)
                     /gene="rclC"
                     /locus_tag="QBH27_004733"
     CDS             complement(2182..2775)
                     /gene="rclC"
                     /locus_tag="QBH27_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDQ7016934.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(2787..3023)
                     /locus_tag="QBH27_004734"
     CDS             complement(2787..3023)
                     /locus_tag="QBH27_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ7016935.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT          893 a          616 c          668 g          866 t
ORIGIN      
        1 ttagacggtg gcagggtagg gtaaatctta tgtgatggag ttacaggcta aaccgggcga
       61 acggggataa aaatttcgca aacaatgtta ttgctatcaa tttcgacgaa gttttcattg
      121 taataaaatc gctcaatatc actgccgttc actctggcta accccctggc aggtaattcc
      181 atcagataga ggtttcgcgc ccattcaggg tagtgctccc actcaccttc atagcggaaa
      241 gaggcataca gtcctcggtt aattgtccac tctttcggca tctctgaatc ggtttgtttg
      301 cataccgtac cggcaaaagt ttcaactgtg agcaaatcga ccgattttgt tgatggttcg
      361 aacgtggaga cgaaatagat atccgtctta aaattcttca tacatctgac taaatgatga
      421 cgtcgcagtt tgacttcttc atccggtaat ttattcgaca cttcaatatc atatttaaat
      481 gaattgccaa aaataggtgt ctcaggaaag ttgactaatc tacactccgt cttttgctgt
      541 tgacgtatta aaaaagaagg gaagatattt gcgagatccc aataatcacg gtggcggtat
      601 tcacggggag agcagccgaa taacttttta aattcgcggc tgaatgactg ctgtgaatca
      661 aaatgcaaag agagtgcaat atcaagcata gatttcgcgg agagccggac aagaatggcg
      721 gcacgacaaa gccttcgttt gcgaatgtat tctcccaaag gcacatgcat gaaatttcgg
      781 aacagaagct ggatgttgcg tctgctgtag ccagatttct gtgcgatatc ttcgattgaa
      841 atagggtgct caagattgca ctcaatccac tccaggagct gctgtagaat cttctgcctg
      901 atcatcacat actccgttaa ttctttgtca tatcgcacat tttcgttatg tcctgggccc
      961 taagtgtatc aaaaacagca agtcccaagg caaaagcact gatagcgtct gcttcattgc
     1021 cccaaaaata gagaggtttc agtcctacat tattaatgaa ttttttgcat aagtgatatc
     1081 aaaatccaca tactaatttg aggttacgtt ttaacgtaga ctcattgttc atacctaatg
     1141 gagggactga cagtggaatt ttccgtatta agtaacaatc tgaaaatgcc gatgatggga
     1201 tttggtgttt ttcaggttac cgataaagac gtgtgcaaac agtcagtgtt gaatgctatc
     1261 cgcacgggtt atcgactcat cgacactgca gcggtatacg gtaatgaaga tgccgtcggt
     1321 gaagccgttc gtgaagccat atctgaaggt ttatgtaccc gagaagagtt atttataacg
     1381 tctaaattgt gggtgcagga tatgttgaat caggatattg cagcagcagg tattgaagca
     1441 tcattaaaaa aatcggggct agaatacttc gatctctatt tattgcacca ggccatgcgc
     1501 gattatttta gtgcgtggcg tgcactcgaa gatgcctatg aagagggcaa attaaaagcg
     1561 attggggttt ccaatttcta ccctcatgtt ctggcgaact tttgtgaaac ggtaagagtt
     1621 aaaccgatgg tcaaccaggt cgagttgcat ccctattttg cccaaccaga ggcgctggca
     1681 accatgaagt attataacgt gcagcctgaa gcatgggctc cgttaggtgg tggacgacat
     1741 aaaccctttg aaaataatct gcttcagagt attgcagatg cccatcaaaa atcgatttct
     1801 caggtcattc tgcgttggaa tattcaacgg ggagtggtcg ttattccgaa atcgacacat
     1861 caacagcgta tcgaagaaaa ttttgctatc tgggatttct cactgacaga gaaagaaatg
     1921 gcgcaaatta gttcgctaga tttgggttat gttggggaat cggtaaaaca ttttaatcct
     1981 gagtttgttc gtggttgtct tgctgtaaaa atacatgatt gatattaatc acttatttta
     2041 cctgagacga caaaaatttt ttaacagggg agtgatattg atcgtcactc tgtcatatct
     2101 ccggtaatat ggcgtcaggc ttcatcaaca gaaagggggc ctgacgcctg aaaaagtgaa
     2161 caacagacag tgttcggatt atcaatattc agtttttaac gttgaactgg attcattact
     2221 gcgttgttta agaatatccc gtgccgaatc tgccattatc atgacagcac ctgccagcat
     2281 cagagtatct ttcaatacca gacgaccagc accggataaa taagggaaac catgatgagc
     2341 atcacccaat gcgggtaccc atgcctccgg agtggtgatt aaaaatgaga gtgttaccag
     2401 cggcgtggta aatgccatca gcccacccaa taaacctaac cagcgattga caggattagc
     2461 caaaaccagc agagcaataa tcacctccac gacgccaaga ccgttggaaa aaccataggt
     2521 attattagcc gtttgccatg ctcttgcttc tggtttgtat tcgccttcgt gagtcagata
     2581 ctgtttatag tcttccgggt gttcataaaa gaacgacatt agtggactgt ttgcgacgaa
     2641 tggtgtaatg ctgtctgcct cgtaagggac aaactttaat aacccaatcc acataaaaac
     2701 aatcgcaatg ctcagacgaa tcaatgtcag gccaatttta tctccccgac ttaacaggtg
     2761 caggtatctt tccattgtaa acatccttat ttatacaata ctgctgttgc ggttgaatta
     2821 ttatcaattc gcatcgatgt aactttccag gatgaagcat tatattttct ggccatatca
     2881 gcaatggcat tctcaagaac tgtcggtgat gctgatactg atgagacgct gatatgtttc
     2941 agaattattt tatcatctgc attggtggaa aatgccataa cgccagataa tagtgttgca
     3001 aataaaacag attttttaaa catataaaat accatcaatg aag
//
LOCUS       DAOFSV010000276         2961 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.276, whole genome shotgun sequence.
ACCESSION   DAOFSV010000276 DAOFSV010000000
VERSION     DAOFSV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.276"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            26..910
                     /locus_tag="QBH27_004735"
     CDS             26..910
                     /locus_tag="QBH27_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDQ7016936.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(961..1815)
                     /gene="yeaE"
                     /locus_tag="QBH27_004736"
     CDS             complement(961..1815)
                     /gene="yeaE"
                     /locus_tag="QBH27_004736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDQ7016937.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1905..2651)
                     /gene="mipA"
                     /locus_tag="QBH27_004737"
     CDS             complement(1905..2651)
                     /gene="mipA"
                     /locus_tag="QBH27_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDQ7016938.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
BASE COUNT          748 a          750 c          695 g          768 t
ORIGIN      
        1 taaaatgaag ttaaaggact gcgtcatgat taagaaaatt tttgcccttc cggtcatcga
       61 acaaatctcc cctgtcctct cccgtcgtaa actggatgaa ctggacctca ttgtggtcga
      121 tcatccccag gtaaaagcct cttttgcatt acagggcgca caccttctct cgtggaaacc
      181 tgcgggtgaa gaagaagttc tgtggttgag taacaacaca ccgttcaaaa atggcgtcgc
      241 cattcgcggt ggcataccgg tttgctggcc gtggtttggt ccggctgcac aacaaggtct
      301 gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg aaatcacatc gtgaagatgc
      361 tgatggtgta gcgctgactt ttgaattgac acaaagcgaa gagacgaaaa aattctggcc
      421 gcacgacttt acgctgttag cgcatttcca cgtgggtaaa acctgtgaaa tcgatcttga
      481 agcacacggc gaatttgaaa ctacctctgc tctgcatacc tactttaacg tgggtgatat
      541 cgctaaggta agcgtcagtg gactaggcga tcgcttcatt gataaagtaa atgacgcgaa
      601 agaagatgta ctgaccgacg gtattcagac cttccctgac cgtaccgatc gcgtgtatct
      661 gaatccgcaa gattgcagcg taattaatga cgaagcgctg aatcgtatta tcgccgtagg
      721 ccaccagcat catctgaacg ttgtaggctg gaacccagga ccggcgcttt cagttagcat
      781 gggcgatatg ccggatgatg gctacaagac ctttgtttgt gtagaaacgg cttacgcttc
      841 agaaacgcaa aaagtcacca aagagaaacc tgcacatctg gcgcaatcca ttcgcgttgc
      901 taaacgttaa tttgcgttaa tattgtgtgc cgggtgcgat gcatccggca cgaatcaaca
      961 tcacaccata tccagcgcag tttttccttt tggtgccgga tacgctttat ccagcataac
     1021 taattccgct gacgaaagtt caacctcaag cacagccgca ttttgttgga catgtgcgac
     1081 agttgccgct tttggaatcg ccatcacacc ctgatggctg attacccacg ccaacaatac
     1141 ttgtgccgca ctgatattgt gagcatgtgc aatttcgttg actaccgcat tttttaacag
     1201 tccattgcgc aaccgcccgg cctgggctaa tggactgtaa gccatcaccg gcatctgctg
     1261 tttctggcac cagggaagta gatcgtactc aatccctcgt gaaccgagat ggtaaagcac
     1321 ctgattagtg gcacactgat ttccccccgg cagctgccag agttcctgca tatcggcata
     1381 atcaaggtta gaaacgcccc agcggcggat ttttccctgg gcgatcaatt tttccatcgc
     1441 tgcgacagtc tcttcgagag cgaaactgcc agaccagtgt aataagtaga gatcgagata
     1501 atcagtattg agacggcgta aactggcttc gcatgcattt atcgcttttt gcccgccagc
     1561 attccacgga tagactttag agacgagaaa gacgttctca cgcagaccga ttaatgcttc
     1621 cccaaccact ttttcggcac cgccatcggc atacatttcg gcggtatcaa tgagggttaa
     1681 accgagttca atgcccgcgc gtagtgcagc aacttctgtt ttacgctgac tggcatcttc
     1741 gcccatatac catgttccct gccctatggc tggcagtgag acatcgccac taaattgaat
     1801 cattttttgt tgcattgttt cctcccaggt attgcaccac cgtaatgcaa aacagggcat
     1861 tacgccctgt ttttatgcac aaaatgccct ggaaagatgc attatcagaa tttgtaggtg
     1921 atcccggtag aaatcaggcc agtccaggat ttatccacca ttgggctgtc agtaacttca
     1981 tcagacagac gggtgtagcg cgcggtaccg taaacactcc agtcgccgag gaagttgtag
     2041 ctggcgctca gctccaggta agggctccag ctgtcgttcg ggttatagcc acgcagaccg
     2101 ctgcgagcgg actctttgcg cgatacgcca taatagtatt cgttctggtt ttcgctgttc
     2161 cactgcacac caatacccgg agtcacggtc aggccaccgt tggtgtaacg atacaaccag
     2221 gccatatccc agacgatgcc gttgctgtta tccagggtat cgccagccag ggtggtacgc
     2281 aggtaaccgt actgggtaaa gtgagcataa gacagaccag ccatcatggt gctcttacgg
     2341 tcatcgaggt gacgcatttg gtgatcgcca ctgtctttag ctttgaagta aagcggcgac
     2401 cagtaagcgg taattgaaag tttgtccgtc gcgtcattcc acaggtagta accaccacct
     2461 aagccacgga accagaagtt atcgccttca tagttgatta ccggtactgg gtaaacatcg
     2521 gtatcgtaat ctttatatgg gtgttcaacg acacctacgc ctgcgcccag ggaaaattta
     2581 ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca ctccaagtgc cagaagtttg
     2641 agtttggtca caattaatca ttccttaaac aaatgtttag cgggcgacaa agtttacccg
     2701 tcaatacatc gaaacccaac ctttttacgt tttcattttt tatagtaact gtttaatttt
     2761 cctgacgcag atgacaccac gcttacagcc aaatgaattt agcgttaccg gcgaccctat
     2821 tatttacatt aattatgcaa aatttatgga tgagttgttg atatgccatt gaaattaaga
     2881 aagccgtgca ggcaagtttt ccatttgcca tctacgctta attttgaagg tgtatcaccg
     2941 ggcacgttgt tctcatcgtc a
//
LOCUS       DAOFSV010000277         2935 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.277, whole genome shotgun sequence.
ACCESSION   DAOFSV010000277 DAOFSV010000000
VERSION     DAOFSV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.277"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1164
                     /locus_tag="QBH27_004738"
     CDS             <1..1164
                     /locus_tag="QBH27_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ7016939.1"
                     /translation="SRYWRLRYRILGKEKTLALGVYPEVSLSEARTKRDEARKLISEG
                     VDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRD
                     ITTLSTPDLLIPVRAAEVKQIYEIASRLQQRISAVMRYAVQSGIIRYNPALDMAGALT
                     TVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDI
                     DNAMWTIPAEREPLPGVKFSYRGSKMRTPHLVPLSQQAVAILAELQTWAGENGLIFTG
                     AHDPRKPISENTVNKALRVMGYDTTKEVCGHGFRAMACSALIESGLWSRDAVERQMSH
                     QERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREECISPFEYAKVNNPLKR"
     gene            1420..2463
                     /locus_tag="QBH27_004739"
     CDS             1420..2463
                     /locus_tag="QBH27_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDQ7016940.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYIWPWILPF
                     SAAGMKHTGIQYQ"
BASE COUNT          812 a          675 c          665 g          783 t
ORIGIN      
        1 tcccgttact ggcgtctccg ttatcgtatt ctgggtaagg agaaaaccct ggcacttggt
       61 gtgtatccgg aagtttctct ctccgaagct cgtacaaaac gggatgaggc ccgaaaactg
      121 atttcggagg gggttgaccc ttgcgaacag aaaagagcta aaaaagtagt ccctgattta
      181 cagctctctt ttgaacatat tgcacgacgc tggcatgcca gtaataaaca atgggcacaa
      241 tcacacagcg ataaagtact caaaagcctc gagactcacg ttttcccctt tatcggcaac
      301 cgggatatca caacactcag taccccggat ctgcttatcc ctgttcgtgc tgcagaagtt
      361 aaacaaattt atgaaatcgc cagtcgtctg cagcaaagaa tatctgctgt aatgcgttat
      421 gccgtacagt ctggcatcat cagatataac ccggctctgg atatggctgg tgcattgacc
      481 acggtaaaac gccagcatcg ccctgctctg gatctttcac gcctgcctga acttctgtcg
      541 cgtattgacg gttataaagg ccagcctgtc acccgactgg cagttatgct gaatttactg
      601 gtttttattc gttccagtga actcagatat gcccgctggt ctgaaattga tattgacaat
      661 gccatgtgga ctattccagc cgaacgcgaa cctctgccag gcgtaaaatt ttcataccgg
      721 ggctccaaga tgcgaacacc acatcttgtg ccactcagcc aacaggctgt agccatactg
      781 gcagaacttc agacatgggc aggtgaaaat ggtctgatct ttacgggagc acacgaccca
      841 cgtaaaccaa tcagtgaaaa taccgtaaat aaggccctca gagtgatggg gtatgacaca
      901 accaaggaag tctgtggcca tggattccgg gcgatggcgt gcagtgcatt gattgaatcc
      961 ggcttatggt caagagatgc ggttgaacgt cagatgagtc atcaggagcg caacggagta
     1021 cgtgccgcgt atattcataa agcagaacat ctggaagaac gtcggcttat gctgcaatgg
     1081 tgggcagatt ttctggatgc aaacagggag gagtgtatca gtccgtttga atatgcaaaa
     1141 gttaacaatc cactgaaaag gtgactgaac gccatttaca cacatgctct tcctcttaag
     1201 gggaagagca cccgcctcaa tcaatttctt ttttatgccg gcggaaccat cagccaggct
     1261 gaggttctca ttcaataact caacgtctta ccacagaaca gaattcactg tattatcatg
     1321 ggcaacgtta atcccggaca ataaaaggac accgatgcca ggcatcacaa tttttatagc
     1381 ctgcaggtta tggtccatcc ttttttacag gttcagaata tgactggcaa attacgcttt
     1441 gaagtgaacg ataaccaggg ctgtttcata tttccggaaa cctggtttgg ctcgttgctg
     1501 gatgagtttg aagaactgat tgatgcatac gatgcggatg aaatcagcga aacaagttac
     1561 atcaacaaac tcaggcgcct tgcccgacag gagaatgact ttattgatgt acacgctcac
     1621 ttggcatacg tatttctgga gcagaatgcc ccgcgtaagg cgctgaatgc tgcccttaaa
     1681 gggctggcaa ttggcaatcg tcttatccct gaaggtttca gtggcagaat catctggata
     1741 catcctgaca accgaccttt tcttcgtgcc ctctatgcgg caatcctggc aaatgcacat
     1801 ttacagcgtc atcaggatgc catcatgctc attgaaaaaa tactggatta caatcccgaa
     1861 gataatcatg gtgcacgctg gctactggga ccggaactgt tacgcactgg cgctcatgaa
     1921 caggcacgcc atatactgca ggaacatgca gatgaatttt caccttactg gtatgaactc
     1981 ggccttctgc attttcttaa tggggaactg gtaaaagctg caactgcatt tcgtcgcggc
     2041 tttgcggcaa atacttacat cgcagaaata ctgtgcggaa atctgcatcc atttccactt
     2101 gctgtatggc ataacttttc aggcggtccg gatactgccg aagattatta tgccacatac
     2161 cacccgttat gggggcaata tccggaagca ttgctgtttg taaactggct ctataatcat
     2221 tcctctgtgc tgcatgaacg ggcagaaatc attaaatgcg ccgaaatgct gatgcaggaa
     2281 gatgatttcg aaatatgcga aagtatttta agacagcagg agaagttgcg tgaaagaatt
     2341 gatgagacgc tttctgagaa aattgtacag aaatgcagaa atatgaatgg tgaatatatc
     2401 tggccctgga tattgccgtt ttcagcggca ggcatgaaac atactggcat acagtatcag
     2461 tagatattgc attagtgtat cctgcacaca agtaataatt tattcaccaa taataacact
     2521 gttaatgccc ccttcccctg gttgtcagcc aggggttatc ttctgaatat ttcttttgaa
     2581 aaggataaca cagtaaatta tttttatgaa ttatcccatg gactcattaa caccctttca
     2641 tgatgcttta ttgtcaaaca cgttatggct gacatcaaaa aaaaccggat ttcctctgcc
     2701 agcgggtaat caccttcccg gtgttttcgg ttggtctggt tactcctgtc tggttattag
     2761 caagataatt gctataaaca gtggaaaact catcgtacat aatctggtga tgaacattac
     2821 gcttattttc ccttgaccgg aagaatcaga ggctgtggtt tcagactgtc tgccagtaca
     2881 ttcctctctc cgttaaaaac cataacgggt tcattatctt cgtctgtcag cagat
//
LOCUS       DAOFSV010000278         2928 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.278, whole genome shotgun sequence.
ACCESSION   DAOFSV010000278 DAOFSV010000000
VERSION     DAOFSV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.278"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(31..2733)
                     /gene="chiA"
                     /locus_tag="QBH27_004740"
     CDS             complement(31..2733)
                     /gene="chiA"
                     /locus_tag="QBH27_004740"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDQ7016941.1"
                     /translation="MKLNIFTKSMIGMGLVCSSLPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIVDGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          725 a          749 c          670 g          784 t
ORIGIN      
        1 cggcgatacc ggaaggtatc gccaacacat ttattgctta gtaaacggcg cgaagatttc
       61 gctaaatgcg tacttcggag cctgttcttt agtcagaccg tggaattccg gagacaggtt
      121 agtgccgccc ggcaggtcgc gcgcgattga ccagatacca accataccga gattacgctt
      181 ctgcgcatcc tgcatgacca gacgagcatc agagagatag aacacctcac cctgaacgtc
      241 gttcacgcca accatcggcg tggtacccat catggcgtca atttctgcat cactcttatt
      301 gccatagagg cctttcagct gcgcatgcag gttggcaatc gcagacgttg cacacttacc
      361 gtggatgttt tggccttcgg tgtttgcaga ctgacaaata gcgttaccgt agtccattgt
      421 catcacgttc acacccgcca gctcaacacc tttcgctttg gcatcgctca ggacattcat
      481 cccttccggc gtcaggcctg tcggcagaat tggcaaggta taccagatag caatatcttt
      541 gccttctgac ttccatttat cctgcacttt cttcacagca aggttacgac gttcaataga
      601 tgcctgatcc gcaacccagg tgccttcgat atcgaagtcc aggactctga ggttcaggtt
      661 atcaacgatg tcataataat gctgcattag atcgtcaacg ttcttacagg aagctgccag
      721 cggggcatta ttagcaccac cgatagacag catcacatca ccgccagcct cacgcagcgc
      781 tttgattttg ctgtactgag cgtaattctg cataccgtaa gcggtccccc atgtcggcag
      841 acaggtgttc gcatctttac tcaccacaaa cgccagcgtg aagtggttga cgttatgatt
      901 cttagccagc gcagccagat ccgggatcgt attcagcgta aagtcgacat acggtgcata
      961 aacgtgtttc ggccacgctt tcttcggcgt acctacgcgc tctttggttc cggtccagtc
     1021 aacaaagaca cgccacgggt tgctgtcaga aggagaaact ttctgcactt tactctgaga
     1081 aatgtagttc tcaccgttaa agcgaatcag cgtatcactg gcataaagcg tttctggatt
     1141 aaactgcggc gcattattca gctcttcgtt gctatagctc tgagtcttac ctaacggctt
     1201 ccacgggcga ctattgctac cggtggcgtt ttggttcgca accagagcag ggttgtcact
     1261 ttgcgtccag aacagcgctt cataagcctg cccatcaaca acaacgcgat cacctttcac
     1321 gtacacggtg gaagcggacc atgctttcgc cggttcgtat ttcttccacg ggttatcgcc
     1381 gccggggaca aagccccatg cgtcttgatc gacagaggtt ttataatgtg catcgttata
     1441 atctacgatg ctgttcgcgc tatacgcttt gctggcctgg aaaccatcca caataatgac
     1501 gccgccatta tcaatttcac aggtagtcgg gttaccgtac tgactcattt ccgcagcggt
     1561 agctgtacgc acgagacgcc atgggttatt ggagttttct acactggtgc cacgcgggca
     1621 atcttcagaa ccaacccacc aggcattctg ataaacatta ccattgaaga taacatggta
     1681 aatttcgctg ccttcctggc cactccacgc ctgcagtgag taatcgttac tgctatccgc
     1741 tggtgttgat ggctcgttgt tatccggagt tggagtaact ggagtttccg gtgtttccgg
     1801 agttggatca accggagtca cagcaccgcc attattatcc ggcttaacgg aacaggagcc
     1861 tgggttacca tactgagtga tttctgttgc cgttgccgcg cgaacataac gccatgggtt
     1921 gctggcatcg ttttctttcg catcgcctgg gcaatttgcc gcgcctaccc accaggcgtt
     1981 cttataaatg ctgccgttgt agataacgta ccatgtagaa gaaccttcct gacctttcca
     2041 ggctacaaca gcatagttgt cgtttgactt atcagctggg gctggctgaa catcaggggc
     2101 cggtttaacg tccggtgccg gtttcacatc cggtgcaggc tgtggtgctg aagtacaaga
     2161 ctgtggatta ctggtttctg agatttccgt tgccgtagcg gcacgaacat aacgccatgg
     2221 gttgctggca tcattgcctt tcgcatcacc cgggcagtta gaagaggcta cccaccaggc
     2281 atttttatat accgcaccat taaagacgac ataccaggtt tgaccgccct gctgtttatt
     2341 ccaggcaact acagaagaat tagacggaac agtgccctgt gctggtgtag ccgaaccgcc
     2401 attagcgggc gtattagatg ctggcgtatt tgaagttgaa gaagatgagc ttccgctctt
     2461 ttcgcaggaa agtgtattgc caaactgact aatttcagca gctgttgcgg tacgctttaa
     2521 acgccacggg ttagttgcat catttgcttt cgcttttccc gggcaatttg tagaagaaac
     2581 ccaccaggca ttttcataaa ttttgccatc gaaaataacc tgatatttat taccaccttg
     2641 ttgattattc catgcttcca ttgccaatgc tggcagagag gaacacacca gccccatacc
     2701 aatcatagat ttagtaaata tatttaattt cattataatc ccttgtaacg taaaaactgc
     2761 aaaacaaaag tcctacgcag gaattattta cgttttacga gaacccttca atatgaatta
     2821 aagtgcggca ttaaaaataa gaaaaaagcc tgacaaatga agcattttta aaacagaaac
     2881 attcatattt aaaatattaa attgaattga tattttaaat atgaataa
//
LOCUS       DAOFSV010000279         2873 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.279, whole genome shotgun sequence.
ACCESSION   DAOFSV010000279 DAOFSV010000000
VERSION     DAOFSV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.279"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            36..1016
                     /gene="btuC"
                     /locus_tag="QBH27_004741"
     CDS             36..1016
                     /gene="btuC"
                     /locus_tag="QBH27_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDQ7016942.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPVNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            1079..1630
                     /gene="btuE"
                     /locus_tag="QBH27_004742"
     CDS             1079..1630
                     /gene="btuE"
                     /locus_tag="QBH27_004742"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDQ7016943.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            1630..2379
                     /gene="btuD"
                     /locus_tag="QBH27_004743"
     CDS             1630..2379
                     /gene="btuD"
                     /locus_tag="QBH27_004743"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDQ7016944.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQNALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            2457..>2873
                     /locus_tag="QBH27_004744"
     CDS             2457..>2873
                     /locus_tag="QBH27_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDQ7016945.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSL"
BASE COUNT          642 a          687 c          816 g          728 t
ORIGIN      
        1 aatcaacgcc atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac
       61 agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct
      121 taagcctttg cgccggtgaa caatggatct cgccaggtga ctggtttact cctcgtggcg
      181 aactgttcgt ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgctg
      241 cgttggctat atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg
      301 gactacttgg cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc
      361 aagggcaact ccccaactgg gcgctggggc tgtgtgcgat tgctggcgcg cttatcatca
      421 ctttaatact cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg
      481 gcgttgcatt agggattatc tgtagtgcac taatgacgtg ggctatctac ttttccacct
      541 ctgttgattt acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc
      601 ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca
      661 ggccggtgaa tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt
      721 ggttctggcg caatgtgttg gtcgcagcga ccggctggat ggttggcgtc agtgtggcgc
      781 tggcgggtgc tatcggcttt attggtctgg tgatcccaca tattctccgg ctgtgtggtt
      841 taaccgatca tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc
      901 tggccgatat tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca
      961 ccgcaacgtt gggtgcgccg gtgtttatct ggttattgtt aaaagcagga cgttagccgc
     1021 aaaaagacgg tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat
     1081 gcaagattcc attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga
     1141 gaagtacgcc ggtaatgtgc tgttgattgt caatgtcgcc tcaaagtgtg gcttaacgcc
     1201 gcaatatgag cagttggaga atattcagaa agcctgggcc gatcgtggtt ttgtggtgct
     1261 gggattcccg tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac
     1321 ttactgtacc accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg
     1381 cgaaggacgc catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc
     1441 ggaagagagc ggattctatg cccgtatggt cagcaaaggt cgtgcaccgc tgtaccctga
     1501 tgatatttta tggaattttg agaaattttt agttggcagg gacggattgg tcatccagcg
     1561 tttttccccg gatatgacgc cggaagatcc cattgtgatg gaaagcatta aactggcgtt
     1621 ggcaaaataa tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg
     1681 ccgctttctg gcgaggttcg ggctggggag atcctgcacc tggtggggcc gaacggcgcg
     1741 ggtaagagta ccttgcttgc acgaatggcc ggaatgacca gcggtaaggg aagcattcag
     1801 ttcgcggggc aaccactgga agcatggtct gcaacaaaac tcgcgctgca tcgcgcctat
     1861 ctttcacaac agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat
     1921 cagcacgata aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat
     1981 gacaaactcg gacgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt
     2041 gctgcggtgg tgcttcaaat cacaccacaa gctaatcccg caggccaatt gctgcttctt
     2101 gatgagccga tgaacagtct tgatgttgcg caacaaaatg cgttagacaa aattctgagc
     2161 gcgctgtgtc agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacattg
     2221 cgtcatgcgc atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga
     2281 gaagaggtgc tcacgccggc aaatctggca caggcctatg ggatgaattt tcgccgtctg
     2341 gatatcgaag gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat
     2401 cgggtaatta cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc
     2461 gtttctgcct tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac
     2521 cgccgccaaa tgccaggctt tctgattcga tcaccgttat tgccggtttg aacgaccagc
     2581 tacaaagctg gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact
     2641 gttcgggatt tgtggttgtg acgatgcgcg atcgtttcga tttgcagctg ccccgtgaaa
     2701 ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga cgagttgctg ccaggtgacc
     2761 tggtcttttt caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca
     2821 acaaccaatt tatccacgcc tctaccagca agggagtgat gcgttcctca ctt
//
LOCUS       DAOFSV010000280         2762 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.281, whole genome shotgun sequence.
ACCESSION   DAOFSV010000280 DAOFSV010000000
VERSION     DAOFSV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.281"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            46..332
                     /locus_tag="QBH27_004745"
                     /pseudo
     CDS             46..332
                     /locus_tag="QBH27_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766809.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
     gene            404..538
                     /locus_tag="QBH27_004746"
     CDS             404..538
                     /locus_tag="QBH27_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDQ7016946.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA
                     "
     gene            835..1089
                     /gene="repA"
                     /locus_tag="QBH27_004747"
     CDS             835..1089
                     /gene="repA"
                     /locus_tag="QBH27_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDQ7016947.1"
                     /translation="MSQTENTVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCNEDGLTQAEVLTALIKSEAQKRCM"
     gene            1195..1329
                     /locus_tag="QBH27_004748"
                     /pseudo
     CDS             1195..1329
                     /locus_tag="QBH27_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615301.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein CopA/IncA"
     gene            1326..1400
                     /gene="tap"
                     /locus_tag="QBH27_004749"
     CDS             1326..1400
                     /gene="tap"
                     /locus_tag="QBH27_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDQ7016948.1"
                     /translation="MTGKVQDFFLCSLLLCNISAGWCD"
     gene            1393..2250
                     /gene="repA"
                     /locus_tag="QBH27_004750"
     CDS             1393..2250
                     /gene="repA"
                     /locus_tag="QBH27_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDQ7016949.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFSSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKFSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRKRQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          734 a          684 c          694 g          650 t
ORIGIN      
        1 ctcaagttag gttgtcccct gaatctccag acaactaata tctgatcgcg ccagtggtgg
       61 ctgttattca ggatggtatc tcagtgcatg tcgatgtggt tatcgttgaa cggcgttcgt
      121 tcagtattgt tgcagcagta tagctggctg atgacagtca tttacgttcg gaacgcagat
      181 gctgggatac tcttctggaa gaggttctga ggcaggctgg cattccgttg ctcagaagtc
      241 acgatgcaag aaaactgctg cagatgaccg aagaatggct gaataagacg caggtttcag
      301 aaatgcgaag accccactcg ttaatccatt aactcgtgag gtctgcatga tgcactttag
      361 cactactgat tgtagcctct tctttgcaaa gatgtcaaag tttatgacga aatataccct
      421 tatcgggttg cttgccgtgt gcgccacggt gttgtgtttt tcactgatat tcagggaaca
      481 gttatgtgaa ctgaatattc acaggggaaa tacagtggtg caggtaactc tggcctgaag
      541 cacggaagta agctgccggg cggggacgga agtccccgct ttccggaagt gtgaggtatt
      601 tcaggggcag acacccgaaa tgctagaaac tgccggtccc gcccggggcc ggtaccctgg
      661 ttaaagcatt tcctactttt cagtcatttt attatcaaaa ttacattaaa cggtcgtaat
      721 cagacatgat ttgtgcgcca acacagatca tcgtcacaac tttcaagtcg ctgatttcaa
      781 aaaactgtag tattctctgc gaaacgatcc ctgtttaagt attgaggagg cgagatgtcg
      841 cagacagaaa atacagtgac ttcctcatct ggcgcaaaac gagcatacag aaaggggaat
      901 ccgctttctg atgcagagaa acaaagatta tcagtggccc gtaaaagagc ttcgttcaag
      961 gaagtaaaag tatttctcga accaaagtat aaggccatgc tcatgcaaat gtgtaatgaa
     1021 gatggcctga cccaggctga agttctgacc gcactgataa aaagtgaagc gcaaaaacga
     1081 tgcatgtgat gatgggctta cattctgagt gttcagaaga ttagtgctag attactgatc
     1141 gtttaaggaa ttttgtggct ggccacgccg taaggtggca gggaactggt tctgatgtgg
     1201 atctacagga gccagaaaag caaaaacccc gataatcttc accaggtttg gcgactaaga
     1261 gaagattacc ggggctaaca agaaactgca tagaagctgt tgctctatgc ggggagtata
     1321 gttatatgac cggaaaagtt caagacttct ttctgtgctc actccttctg tgcaacataa
     1381 gtgcaggatg gtgtgactga tcttcaccaa acgtattacc gccaggtaaa gaacccgaat
     1441 ccggtgttca caccccgtga aggtgccgga acgttgaagt tctgcgaaaa actgatggaa
     1501 aaagcggtgg gcttcagctc ccgttttgat ttcgctattc atgtggcgca tgcccgttcg
     1561 cgtggtctgc gtcgacgcat gccaccggtg ctgcgtcgac gggctattga tgcgctgctg
     1621 caggggctgt gtttccatta tgacccgctg gccaaccgcg tccagtgttc catcaccacg
     1681 ctggccattg agtgcggact ggcgacagag tccggtgcag gaaaattctc catcacccgg
     1741 gccacccggg ccctgacgtt cctgtcagag ctgggactga ttacttacca gacggaatat
     1801 gacccgctta tcgggtgcta cattccgacc gatatcacgt tcacactggc gctgtttgcc
     1861 gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc gcagccgtgt ggtatgggaa
     1921 aacaaacagc gcaaaaagca ggggctggat accctgggta tggatgaact gatagcgaaa
     1981 gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaaa cagagcttaa gtcccgggga
     2041 ataaagcgtg cccgtgcgcg tcgtgatgcg aacaggaaac gtcaggatat cgtcaccctg
     2101 gtgaaacgac agctgacgcg tgaaatctcg gaagggcgct tcactgccaa tcgtgaggcg
     2161 gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga ttctgtcacg taaccgcaat
     2221 tacagccgcc tggccacagc ttccccctga aagtgacctc ctctgaataa ttcggcacgc
     2281 gccggaggca ttcgcacgcc tgaagcccgc cagtgcacaa aaaatcagca ccacatacaa
     2341 aaaaacaacc tcatcatcca ccttcaggtg catccggtcc cccttgtttt tgatacaaaa
     2401 cacgcctcac agacgggtaa ttttgcttat ccacattaaa ctgcaaggga cttccccata
     2461 aggttacaat cgttaatgtc ataaagcgcc agccgtcagc cctacagggt gcaatgtatc
     2521 ttttaaacac ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa
     2581 cctattcact gcttgtccta tggacagaca gatatgcacc tcccaccgta aacggcgggc
     2641 cccaaccgga gccactttag ttacaacaca caaaaccaac ctccagaaaa accccgaacc
     2701 agcgcagaac tgaaaccaca aagcccctcc ctcataactg aaaagcggcc ccgccccggc
     2761 cc
//
LOCUS       DAOFSV010000281         2678 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.282, whole genome shotgun sequence.
ACCESSION   DAOFSV010000281 DAOFSV010000000
VERSION     DAOFSV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.282"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(57..530)
                     /locus_tag="QBH27_004751"
     CDS             complement(57..530)
                     /locus_tag="QBH27_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDQ7016950.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDYDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(624..869)
                     /locus_tag="QBH27_004752"
     CDS             complement(624..869)
                     /locus_tag="QBH27_004752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016951.1"
                     /translation="MQHVPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            complement(869..1687)
                     /locus_tag="QBH27_004753"
     CDS             complement(869..1687)
                     /locus_tag="QBH27_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDQ7016952.1"
                     /translation="MRLTSRFGYVNQIRRDRPLTHEELMHHVPGIFGEDRHTSCSERY
                     TYIPTITVLESLQREGFHPFFACQTRVRDPDRREYTKHMLRLRRNGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(1908..2318)
                     /locus_tag="QBH27_004754"
     CDS             complement(1908..2318)
                     /locus_tag="QBH27_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016953.1"
                     /translation="MKTFIKTLLVAVTILFSVFAAAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKEQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            complement(2334..>2678)
                     /locus_tag="QBH27_004755"
     CDS             complement(2334..>2678)
                     /locus_tag="QBH27_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016954.1"
                     /translation="YELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVT
                     INGTVWLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEF
                     AYLVKNYVAAVN"
BASE COUNT          619 a          686 c          705 g          668 t
ORIGIN      
        1 ctggttgtca tttctccggg cagaaaggaa atctgttgca ttgttccggc cccctttcag
       61 tcaataatgc gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagacgg
      121 taataatgaa ccgtcatggc ataacactcc gtacggcagg cgtgatgact gtacgtcatc
      181 agacaggcgg caatgccggc ggcttccggg ctcatttcag cgcggttacc gttcatggca
      241 ttgaacagta cccatgtttc gtcatcatag tcatccggtt caggggccat aaatgccccg
      301 ccgttgttca gggtgtacag attccagata ccaccgcagt agttttcgca cagacggtcc
      361 atccagccga agacacgggg ctccagggtc acccactgtg gaatgaggcc aaaatgctgc
      421 ggccagaagc tgatgcgctg ttcatcaggg actatggtgg caaccagttg ttgcaggcca
      481 gtctccggtg cacaggccga agaggtggtg ttctgagaca atgttttcat gtatgaaatc
      541 cttgtaatgt gaataaatga gagggagtaa aggtcgggag ctggcaacat cacgttcatc
      601 actgaccgtg agacccggta atatcaggga tgaatgatat tcggctgaaa ttccggacag
      661 ccgtacgcgt aaccgttccg gtcacagacg gatggccaga tgccattgcc tgtactcagt
      721 ctgcacagac tgatttctcc gggacggtaa agcagacggg tacaccaggc gcacaggtca
      781 tcatcagaaa tggaacgaca ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc
      841 tgaatcacgc cttcagggac atgctgcatc agcgcaggct ctccagcagc gtttctgcca
      901 ttacccacaa tgcgcggttg agcttaatat cggtgtcgat gctgtgaatg gcacgggtgt
      961 ggatacgttt tccttttgca ctgcgaccgg aaatcccgcc tttcagcata ttctcctgga
     1021 tggtctgata agtactccac aggtccttac cgtaatcctc ccggcgtcgt ggcgtcagaa
     1081 tgtcggcggt ggtgacgggc cgatgttcgt caccataacg gtaagtcagt gccgcctgtg
     1141 ccagcgcctg gcgtgccggt ggcggcagaa tcagcgactg catggcatca cgcttctcct
     1201 ctatccggtc aaacacgccc accacctcgt aagccccttc aatgactctg tccaccacat
     1261 ttccccggtg cggaacacgc acttccccca gagactgacc acagacgcat ccgttctggc
     1321 agacgaacct gaagtaaccc ggcagcatct ggtagctgga ggtaccgtca tgagagttga
     1381 gcagaataat ttcagggaca tgttctccgt ttatctctcc gttacggcgc agacgcagca
     1441 tgtgtttggt gtattcccgg cggtccgggt cacgtacgcg ggtctggcag gcgaagaatg
     1501 gatgaaagcc ttcccgctgc aggctttcca gtacggtgat ggtggggatg tacgtatagc
     1561 gttcactgca ggaggtatgc cggtcttcac cgaaaatacc cggtacatgg tgcatcagtt
     1621 cttcgtgtgt cagcggacgg tcacggcgta tctggtttac ataaccaaaa cgactggtta
     1681 atcgcatgat ttgctcctta tcggtggtta agatttactg gtgtaataaa tgaaaaaagc
     1741 cacgtctccc ggagaagacg cggcctgacg ggtgaaatga atgaggttta ttgtctgaga
     1801 agcctttcac tggcgagcca cgcattaagc ggctttccgg caacaatggc tggggctgta
     1861 tttacctttg atattaataa tgtgcagaga aacgcatggt ttactcctca ggttttaagt
     1921 ttgtccagag ccacaacgac aggttttccg gatgtgtcga tgactcctac cgtcggtacg
     1981 ccatttttga tttcgaagaa cggtttgttg ctacctgagc acagctggtt gtctttgtat
     2041 tcgagcatct gttctttccc ggttttcagg ttaagtaccg agacagtgta gtcgtaacac
     2101 caggcaccac cacgttcaag cacgaaaaca tagctgttat ctttatagtt gaacggattc
     2161 ttcgtctggc aattcatacc gtcaatctca gtacaggaaa acgcctctgt agtattaacg
     2221 tatttgatgt tgtttggcag ttttacttgt ttcgccgcag cgaagacaga gaacagaata
     2281 gttacagcaa cgagtaaagt tttgataaaa gttttcatga cagactcctt aaattagtta
     2341 accgcagcta cgtagttttt caccagatac gcgaattcat gcacgccaat ttcgccgaac
     2401 caaaccaggt aggccgggtc atctaaacca aagtagcggt agccaacgac ttcatgggtt
     2461 tttgggttat agagtgttac gataaagtcc cagccgcagg tattggcttt gcaaccgtcg
     2521 ccctggagcc agactgtacc gttaatggtt actggcgtta cgtttccgcc agggccatcc
     2581 atatacccgc ctttaccgaa catgccgctc agtccgaccg gttttaatgc tttgtcaagc
     2641 gcagctttgt aggcagcgtc accttcgtac aactcgta
//
LOCUS       DAOFSV010000282         2652 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.283, whole genome shotgun sequence.
ACCESSION   DAOFSV010000282 DAOFSV010000000
VERSION     DAOFSV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.283"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..2559
                     /locus_tag="QBH27_004756"
     CDS             <1..2559
                     /locus_tag="QBH27_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDQ7016955.1"
                     /translation="VEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQ
                     TVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREIMLSSSGT
                     TLISLVDGSGNPVSVEVQSVTDGVKVKVSRIPDGVAEYSVWGLKLPTLRQRLFRCVSI
                     RENDDGAYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGE
                     YQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTAETTYRFRQLALGRYTLTV
                     RAVNARGQQGDPASVSFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSE
                     KRITNTAQVEKSARYLGTGSQWTVQGDRIKPGMDFWFYVRSVNLVGKSAFVEASGQAS
                     NDAKGYLEFFRGLIDETLLGQALKKRIDASALRSDVTQLEENIGQLEEDIRQRMDTDI
                     AEVTRKIGEAENSLTQLVAKKNEDQTLAIAQVSQKVDRVSSEISQTVSQSSEENTRQI
                     AQVRQYVDKKQSEIKQTVSQGQSENARQIAQVRQYVDEKGSEIASTTDKKLGDQAVTI
                     QQIQRVQSDTRDNLNAMYMLKVQKTKKGIPYVAGIGAGIEDVDGQTLSNILLQADRIA
                     MITPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTARNAD
                     ISGAITANTGTLNNVTINKNCVIRGKLSANQIEGDLVKTVGKAFPRDSRAPKRWPSGT
                     ITVRVYDDQPFNRQIVIPAVAFSGARHERENSDTYSSCRLIVKKNGAEIYNRTAMDNT
                     LVYSGVIDMPAGRGDMTLEFSVSAWWVNGWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          649 a          644 c          830 g          529 t
ORIGIN      
        1 gtggaggaca cgcaggccat tgcccgttac ggtcgtaacg tcacgaagat ggatgccttt
       61 ggctgtacca gtcgggggca ggcacaccgc gccgggctgt ggctgattaa aacagaactg
      121 ctggaaacgc agaccgtgga tttcagcgtg ggtgctgaag ggcttcgcca tgtaccgggc
      181 gatgtcattg aaatctgcga tgatgactat gcggggatca gcaccggtgg tcgcgtgctg
      241 gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcatgct gtcatcctcc
      301 ggcaccacgc tgataagcct ggttgacgga agtgggaatc cggtcagcgt ggaggtccag
      361 tccgtcaccg acggcgtgaa ggtgaaagtg agccgtattc ctgacggcgt tgccgaatac
      421 agcgtgtggg ggctgaagct gccgacgctg cgccagcgcc tgttccgctg tgtgagtatc
      481 cgggagaacg atgacggtgc gtatgccatc accgccgtgc agcatgtacc ggaaaaagag
      541 gccatcgtgg ataacggagc gcactttgac ggcgaccaga gcggcacggt gaatggtgtc
      601 acgccgccag cggtgcagca cctgaccgcc gaagtcaccg cagacagcgg ggaatatcag
      661 gtgctggcgc gctgggacac gccgaaggtg gtgaagggcg tgagcttcct gctccgtctg
      721 accgtaacag cggatgacgg cagtgagcgg ctggtcagca cggcccggac ggcggaaacc
      781 acttaccgct tcaggcagct ggcgctgggg cgttacacgc tgacggtccg ggcggtaaat
      841 gcccggggac agcagggcga tccggcgtcg gtatcgttcc ggattaacgc acctgcaaaa
      901 cccgccacca ttgagctgac gccggggtat tttcagataa cggcggtccc gcgtcttgcg
      961 gtgtatgacc cgacggtaca gtttgaattc tggttctcag aaaaacgcat cacgaacacg
     1021 gcacaggtgg aaaaatctgc ccgttatctg gggaccggca gtcagtggac tgtccagggc
     1081 gatcggatta agccggggat ggatttctgg ttttatgtgc gcagtgtcaa cctggtgggc
     1141 aagtctgcat ttgtggaagc cagcgggcag gccagcaatg atgcaaaagg gtatctggaa
     1201 tttttccgtg gtctgataga tgagacgctg ctggggcagg cactgaaaaa gcgcattgat
     1261 gcgtcagccc tgcgatcgga tgtcactcaa ctggaagaga atatcggaca actggaagaa
     1321 gacatccgtc agcggatgga cacggatatt gcggaagtga cccggaaaat cggggaggcg
     1381 gaaaacagcc tcacgcagct ggttgcgaaa aagaatgagg atcagacgct ggccatcgcg
     1441 caggtgagtc agaaagtgga ccgtgtcagc agtgaaatct cacagacggt cagtcagagc
     1501 agtgaggaaa atacccgaca gatagcacag gtccgccagt acgtggacaa aaagcaaagt
     1561 gaaatcaagc agacagtcag ccagggccag tcagaaaacg cccggcagat agcacaggtc
     1621 cgtcagtacg tggatgaaaa agggagtgaa attgcctcga ccacggataa aaaactgggt
     1681 gaccaggccg tgaccataca gcaaatccag cgggttcagt cagacacgcg cgataatctg
     1741 aatgccatgt atatgctgaa ggtgcagaaa acgaagaagg gtattccgta tgtggccggg
     1801 attggcgcgg ggatcgagga tgtcgatggt cagacgctga gtaacattct gctgcaggcc
     1861 gatcgcattg cgatgattac tccggagaac ggtaacacca cgccgttatt tgtggcgcag
     1921 gggaatcagc tgttcatgaa cgacgtgttc ctgaagcgac tgtttgcggt gagcatcacg
     1981 tcatccggca atcctccgac gttttccctg acgccggatg gcaggctgac agcccgcaat
     2041 gcggatatca gtggagccat cacggcgaat accggcacgc tcaataatgt caccattaac
     2101 aagaactgtg tcatcagagg gaaactgtct gcaaaccaga ttgaaggcga tctcgttaaa
     2161 acagtgggta aggctttccc ccgtgactcc cgtgcaccga agcgttggcc atcaggaacc
     2221 attaccgtca gggtttatga cgatcagccg tttaaccggc agattgttat tccggcggtg
     2281 gctttcagcg gtgccagaca tgagcgggag aacagcgata cttattcgtc atgccgcctg
     2341 atagtgaaga aaaacggtgc tgaaatttat aaccgtaccg cgatggataa tacgctggtt
     2401 tacagtggtg ttattgatat gcctgctggt cgcggcgaca tgacgctgga gttttctgta
     2461 tcagcatggt gggtaaatgg ctggtatccc acagcaagta tcagcgattt gctggttgtt
     2521 gtgatgaaga aagccactgc aggcatcacg attagctgaa ttttataacc ccaatacggg
     2581 cgccagaaat ggcgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaattatg
     2641 tgctgttatt ct
//
LOCUS       DAOFSV010000283         2593 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.284, whole genome shotgun sequence.
ACCESSION   DAOFSV010000283 DAOFSV010000000
VERSION     DAOFSV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.284"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1194
                     /locus_tag="QBH27_004757"
     CDS             <1..1194
                     /locus_tag="QBH27_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDQ7016956.1"
                     /translation="VQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDC
                     GETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRA
                     ASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVREN
                     WQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVND
                     GFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNR
                     KLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQS
                     KTWVLFGKTNHGKLSGEILYWLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAM
                     AFASI"
     gene            1284..1667
                     /locus_tag="QBH27_004758"
                     /pseudo
     CDS             1284..1667
                     /locus_tag="QBH27_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1696..2100
                     /locus_tag="QBH27_004759"
     CDS             1696..2100
                     /locus_tag="QBH27_004759"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDQ7016957.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            2097..2444
                     /gene="tnpB"
                     /locus_tag="QBH27_004760"
     CDS             2097..2444
                     /gene="tnpB"
                     /locus_tag="QBH27_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDQ7016958.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          656 a          652 c          722 g          563 t
ORIGIN      
        1 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
       61 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
      121 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
      181 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      241 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      301 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      361 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      421 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      481 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      541 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
      601 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
      661 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
      721 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
      781 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
      841 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
      901 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
      961 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1021 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt actggttggt ggggcaaggc
     1081 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     1141 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     1201 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     1261 actacgccct gcctcttact ctactcagca catggcaggg ggtcgtctat gtggcgttca
     1321 tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct atggaaacga
     1381 cattcgtgct ggatgctctg gagcaggcgt tatgggcccg tcgaccgtcc ggcacagtcc
     1441 atcacagtga taaaggttct cagtatgtat cgctggccta cacacagcgg cttaaggaag
     1501 ccggattact ggcatcaaca ggaagtactg gtgactcgta tgacaacgcg atggcggaga
     1561 gcatcaatgg cctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccgtaagc
     1621 gtcgtctcag caccgtctgg cagatcctga aattcctgag agaatagtgg acaccaaata
     1681 tggtggacgc tatccatgaa atcattaacc gcagtgcgta aaaaaagccc taattatccc
     1741 gttgagttca aaatcaaaat ggttgaactc tcgcatcgac cagagatctc cgtagcgcaa
     1801 ctcgctcgtg agcatgggat caacgataat ttgctgttca agtggcgcca gtactggcgc
     1861 gaaggaaaac tacgtcctcc ttcaacaaca gaaaacaacg tgcctgagct gctcccgata
     1921 actcttgatg ccgaagatgt tgtccctaca acctcccccc ggtcacaacc tgtagctgct
     1981 gcgacacctg aatcactcaa tatcagctgt gaagtaacgt tccggcacgg atcactccgt
     2041 ctgaatggtg ccatcagcga aaatatcctg aacctgctga tacgggagct caaacgttga
     2101 tcccattacc atcagggaca aagatctggc tggtcgctgg catcaccgat atgagaaacg
     2161 gcttcaacgg cctggcggca aaggtgcaga cgacgctgaa agacgatccg atgtcaggtc
     2221 acgtttttat cttccgtggg cgtaatggca gtcaggtaaa gctcctctgg tctaccggcg
     2281 atggactgtg tctgctgacc aaacggctgg agcgcggccg cttcgcctgg ccgtcagccc
     2341 gggatggcaa agtgttcctc acaccggcac agctggcgat gctccttgaa ggtatcgact
     2401 ggcggcagcc taaaagactg cttacgtccc tgactatgtt gtaagcctct ttatcctggt
     2461 cgacgctgaa tgagcctggt aatatacccg gtatgagcag ctcacttcct gacgatatca
     2521 atgcactgaa acgtctcctt gccgaacagg aggcgctgaa ccgtgccctg ctggaaaagc
     2581 tgaacgagcg tga
//
LOCUS       DAOFSV010000284         2575 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.285, whole genome shotgun sequence.
ACCESSION   DAOFSV010000284 DAOFSV010000000
VERSION     DAOFSV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.285"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(164..808)
                     /locus_tag="QBH27_004761"
     CDS             complement(164..808)
                     /locus_tag="QBH27_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016959.1"
                     /translation="MNGKYNVRSELLARCIGTGRLKGDVVSDFIGFNGSKQIGYVLLT
                     LFLIKVINPDLLSHYRIFNRFLRYERKVMDIYNSLSDIEVDCICREVMAIYEHTQRCC
                     NEKKITTVQLGRKLNGRYADMIAELKETAEMRGEGVISFEMDILNSFNDANEYHGRVK
                     LELDIPASDILYCHDFIDSEHVNSWLVEPHEWVVINRSLTGIVTVPVSAIKISY"
     gene            complement(1002..2450)
                     /locus_tag="QBH27_004762"
     CDS             complement(1002..2450)
                     /locus_tag="QBH27_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ7016960.1"
                     /translation="MRIRSLDDAATTEETLLTLALTPLVKELKALECNKESQISKVKA
                     ALVKAVNEIAEPHQERFSSISRQIQTGFQHVFPALGVQLSVEMNPPELKIDNLLKQGS
                     GLLVKEAAGNSRIGQQGTGARRALFWAMLQVHNEISRQNEKREALLKSFREQLKKEVR
                     KNVKSENINLLKQQIDAIENGAPVPEDTDDPALPGYILLMDEPENALHPMAARAAQAH
                     LYELGKHPDWQVLLTTHSPYFINPLEDHTTIARMQRSTDGKSLSPRLYVADEANFSPD
                     EKDNLQALQLTDIGFAEIFFGSYPIIVEGDTEHAAFISAITKEKHEMSGKVSIVRARG
                     KAVLVPLIKMLNHFKADFGIVHDIDWPYRRYGSNNGSWTLNTIIRNEIIKCRNNGKKV
                     YHRWSAPDFERFLGGEELGKDKPYTAFNRISRDEKLKEKIQNLIINLFEGECYDPDDF
                     EPDDDFNAQLMEQLKIWAKNNGESDNVRVMGC"
BASE COUNT          799 a          579 c          362 g          835 t
ORIGIN      
        1 ctttctccgt taataaatta tcattcattt atttttactt tatgcaaaag aaacccgttg
       61 atatctccaa tacatagcaa caaacattaa ataatattta attaattatt acagagatcg
      121 tttttaccct tatatataaa gtaagctaca tacataaatt ttttcaataa gagattttta
      181 ttgctgatac tggcactgta acaatcccag taagagaacg attaataacc acccactcat
      241 gtggttcaac cagccaggag tttacatgtt ctgaatcaat aaaatcatgg caatataata
      301 tatctgaagc aggtatatcc aattccagtt taacacgtcc gtgatattca ttcgcatcat
      361 taaaagaatt caatatatcc atctcaaaag aaattacccc ctcccccctc atctctgctg
      421 tttcttttaa ctcagcaatc atatctgcat acctgccatt aagtttcctg cctaactgga
      481 cagttgtaat tttcttttcg ttgcagcatc gctgagtatg ttcatatata gccatcactt
      541 cccggcaaat acaatcaact tctatatcac tcaaagaatt atatatatcc ataacttttc
      601 tctcataacg caaaaaacga ttaaatatac gataatgaga gagcaaatca ggatttatta
      661 cttttattaa aaataaagtt aataaaacat aacctatctg tttgctacca ttaaacccaa
      721 taaaatcact taccacatct cctttcaatc tacctgttcc gatacaacga gctaacaatt
      781 cactcctgac attatattta ccattcatat tcgccacact atcctgaaca acaaatgatc
      841 accaaaatgc gtttaaatac cctacaaaca aactatctca ctgaataaat tgcaacactt
      901 ccccttccac aacgtcctaa aggctttata taaaaacagc aaaaacgaat tagttatgca
      961 aagtcattta tatgaaaccc agccattaac caatgttata atcagcatcc cataacgcga
     1021 acattatcac tttcaccatt attttttgcc catattttta attgttccat gagttgagca
     1081 ttaaaatcat catcgggttc aaagtcatca ggatcataac actccccttc aaacaaatta
     1141 attataagat tttgaatctt ttcttttaat ttttcatcgc gcgaaattcg attaaatgct
     1201 gtatagggtt tatctttccc caactcctca ccacctaaaa aacgctcaaa atcgggggca
     1261 ctccaacgat gatatacttt tttcccatta ttcctgcatt ttataatctc atttctaatg
     1321 atcgtattaa gcgtccatga accattattc gaaccatatc tacgataagg ccaatcaata
     1381 tcatgtacaa tcccaaaatc tgctttaaaa tgatttaaca tctttatcaa aggaaccaga
     1441 accgccttac ctctggctct gacaatactt accttacctg acatctcatg tttttcttta
     1501 gtgattgctg aaataaatgc cgcatgttca gtatcacctt caacaataat tggataactc
     1561 ccaaaaaata tttcagcaaa accaatatct gtaagttgta gtgcctgtaa attatctttc
     1621 tcatcaggtg agaagttagc ttcatcagcc acatacaagc gtggagaaag tgatttccca
     1681 tctgttgaac gttgcatacg agcaatcgta gtatgatcct caagagggtt tataaagtat
     1741 ggtgaatgag ttgttaatag cacctgccaa tctggatgtt taccaagttc ataaagatgc
     1801 gcttgcgccg ctctggcagc cattgggtgt aacgcatttt ctggctcatc cattaaaaga
     1861 atatatccgg gcaaggctgg atcgtctgta tcttcaggaa caggggcgcc attttcaata
     1921 gcatctattt gttgtttcag cagatttata ttttcacttt ttacattttt cctcacctct
     1981 tttttaagtt gttctctaaa ggatttaaga agagcttctc gcttttcatt ttgtcggcta
     2041 atttcattat gaacctggag cattgcccaa aaaagagctc gtcttgctcc tgtcccctgt
     2101 tgcccaatgc gagagtttcc cgcagcctct ttgactaata aaccagaccc ctgctttaat
     2161 aaattgtcta ttttaagctc aggtggattc atttcaacac tgagctgaac accaagagca
     2221 ggaaagacat gttgaaaccc tgtctgaatt tgtcgagaaa tagaagaaaa tcgttcttga
     2281 tgaggttcag cgatctcatt tacagcttta accaaagctg ctttaacttt tgatatttgt
     2341 gactctttat tacactccag agccttcaac tctttcacta agggagtcaa cgcaagagtg
     2401 agcaatgtct cttctgtagt cgctgcatca tccagtgatc taattcgcat tggtcttggc
     2461 aaacgtgatt taaaaacatt atcggctcca ccggccttgc tgtcctctga ccagtcttca
     2521 gtctgaggat cccatgtttt tcttgttggt tttcccgttt catcccaaac ccata
//
LOCUS       DAOFSV010000285         2514 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.286, whole genome shotgun sequence.
ACCESSION   DAOFSV010000285 DAOFSV010000000
VERSION     DAOFSV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.286"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            158..1699
                     /gene="istA"
                     /locus_tag="QBH27_004763"
     CDS             158..1699
                     /gene="istA"
                     /locus_tag="QBH27_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HDQ7016961.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1714..2460
                     /gene="istB"
                     /locus_tag="QBH27_004764"
     CDS             1714..2460
                     /gene="istB"
                     /locus_tag="QBH27_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDQ7016962.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          718 a          590 c          605 g          601 t
ORIGIN      
        1 ccatcgagat cgggtaataa catttgaaca gatcgctgaa taacatcgat ggagatcact
       61 tttgactcat tttgttattc agtgatctcc atcaatgtta ttggaacttc acaggtgtgt
      121 tgatctgtat cttttgccat tccggtaaag gatacctatg ccaacagttc caatttctat
      181 gagaaaactt aaagaaattc ttaggcttaa atacggtgtt ggactcagcc atcgacaaat
      241 tggtcgtagt cttgcaatct ccccttccgt tgtatccaga tatgctaatc gggcggctca
      301 acttggcata aagcagtggc ccttacctac aggatgggat gatacaaaac taaaacatgc
      361 gttccttcag acccaggtta agatgaagaa gcactctctg cctgactggg ctacagtaca
      421 ccgggaactg cgtaataaat gcgtgacgct gcagctactc tgggaagaat actgtgagcg
      481 taatccaggc ggtttttaca gctataacca ttactgccgg atgtaccgtg aatggctcaa
      541 aaccacttca ccatcaatgc gtcaggtaca taaagctggc gaaaaacttt tcgttgatta
      601 ctgtggacct accgttggcg ttaccgaccc tgagaccgga gaaataagaa ctgctcaggt
      661 catcgtagct gttctcgggg catcaagtta cacatgggca gaggccacct ggtctcagca
      721 gcttgaagac tgggtgatga gtcatgttcg ctgcttccag tggttgggtg gcgttcctga
      781 acttgttgtt ccggacaatc tgaaaagcgc cacatccagg gcatgtaagt atgatcctga
      841 cgttaaccct acctaccagc agatgcttga gcattataat gtcgcagttt tgcctgcgcg
      901 gccacgtaaa ccgaaagata aagccaaagc tgaagttggc gttcaggttg ttgaacgctg
      961 gatcatggcc cgaatcaggc atgagatctt ctacagcctt gcatcgctta atcagcgcat
     1021 tcgggagttg ctggaaagac tgaataacaa aataatgcag aagttgggtt attcacgtgc
     1081 agaactcttc atccagcttg ataaacccgc actgaagcct cttcctgaag ccagttacag
     1141 ttacaccctg gtgaagaaag tcagagttca tgccgattac cacgtggaaa tcgacaaaca
     1201 ttactactcg gttccatgtt cgctgttagg ccagcaactg gaagcatgga tctccggaga
     1261 actggtaaga ctcttcaatc aggggcagga ggttgctgtg cacccgcgca agcgtactta
     1321 tggctacagt acccgcaacg agcacatgcc tgaagctcat cgacagcatg ccacctggac
     1381 gccagagcgt cttctggaat gggcggggca cataggcagt gaaactcata gttatgtgct
     1441 tcatatactg aactctcgtc cacatccgga acaaagctat cgcttctgcc ttggactcct
     1501 gaaccttcat aaaaaataca gtaaagccag acttaatgca gcatgtgcaa gagctctgaa
     1561 aacaaaggta tggcgtctgt caggtattaa atcgatcctg gaaaaaggtc tggataaaca
     1621 acctgttcag gatccaaaac cagatctgtt atccacgatg gaacacgaaa acgtacgcgg
     1681 cagtgagtat taccactgat acgggatcca atgatgaatc atctttacga acaactgacc
     1741 gcacttaaac tcaccggctt ccgtgatgcg cttaaaaagc aacttgctca accgggcaca
     1801 taccaggagc tgggcttcga agaacgcctg tcattactga cagcagaaga actaacctgc
     1861 cgtgaaaaca ggaaggcaga gcgtctgatc aaacatgcac ggttcagact taatgctgag
     1921 ttatcaaagc tggattatcg taacaataga gggctggaca gggccctcat ccgttcactc
     1981 agtcagggaa actggttaac cctgaaacaa aatattttac tgaccggggc caccggcagc
     2041 ggtaaaacgt tcctggcatg tgcacttggt cataatgcct gccgacaggg atacaaggtc
     2101 tactattatc gccttaaagc gctgatggaa cagtgctatc aggggcatgc tgatggaaga
     2161 tacagcaaac ttttgaccag gctgaataat agcgatctgc tgcttctgga tgactggggg
     2221 ctggaacctc tctcatcaga acagcgtagc gacctgctgg aaatagtgga tctgatgtac
     2281 caacgaggct caatcatcgt agtgagccag ttgccggtgg aaaactggta caaaatgatc
     2341 ggagactcca cacatgcgga tgccatccta gatcgactgg ttcatggcag tatcaagatc
     2401 gaacttaaag gagaatcaat gcggaaaata caatctccgt tgaccgaagg agatcagtga
     2461 aggtaattta aaaacggttc tgtgaaagtg acacgaaccg atctccatcg atgt
//
LOCUS       DAOFSV010000286         2508 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.287, whole genome shotgun sequence.
ACCESSION   DAOFSV010000286 DAOFSV010000000
VERSION     DAOFSV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.287"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            61..930
                     /gene="hexR"
                     /locus_tag="QBH27_004765"
                     /gene_synonym="yebK"
     CDS             61..930
                     /gene="hexR"
                     /locus_tag="QBH27_004765"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDQ7016963.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            1058..2500
                     /gene="pyk"
                     /locus_tag="QBH27_004766"
     CDS             1058..2500
                     /gene="pyk"
                     /locus_tag="QBH27_004766"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDQ7016964.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
BASE COUNT          629 a          611 c          658 g          610 t
ORIGIN      
        1 aactgcattt cgatttcttt cagagcggaa atcgtcatta cccgtgagtc tctttacatc
       61 atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc tgagcgcaaa
      121 gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctctg
      181 gcgctggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacaca
      241 cgcggttttc ctgattttaa acttcatctg gcacaaagtc tggcgaatgg cactccctat
      301 gttaatcgca acgtcaatga agatgacagc gttgaatcat acacggggaa aatatttgag
      361 tccgcaatgg caacgctcga tcatgtccgt cattcactgg ataaatctgc catcaaccgc
      421 gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ttcttcagcc
      481 gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc
      541 gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg
      601 ctgatttctc acacgggaag aacaaaaaat ctggtcgagc tggcgcagct ggcacgcgaa
      661 aacgacgcta tggtaattgc cctcacctct gcgggtaccc cactcgcccg ggaagcaacg
      721 ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga
      781 cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg gcgcggtaca
      841 aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag
      901 cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg
      961 acatccgaat gagattaatt tatcgccatc acggcgttat ttcattcgga tttcatgttc
     1021 aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac
     1081 taaaatcgtt accacgttag gcccggcaac agatcgcgat aataaccttg aaaaagttat
     1141 cgcggcgggt gccaacgttg tacgtatgaa tttttctcac ggctcgcctg aagatcacaa
     1201 aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct
     1261 gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct
     1321 caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga
     1381 aaaagtcggt atcgactaca aaggcctgcc tgctgacgtt gtgcctggtg acatcctgct
     1441 gctggacgat ggtcgcgtcc agttaaaagt actggaagtc cagggcatga aagtgttcac
     1501 cgaagtgacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg
     1561 tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat
     1621 tggcgtagat tacctggctg tctccttccc acgctgcggc gaagatctga actatgcccg
     1681 tcgtctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga
     1741 agccgtttgc agccaggagg caatggatga catcatcctc gcctctgacg tggtaatggt
     1801 tgctcgcggc gacctcggtg tggaaattgg cgatccggaa ctggtcggca ttcagaaagc
     1861 gttgatccgt cgtgcacgtc agctaaaccg cgcggtaatc acggcgaccc agatgatgga
     1921 atcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt
     1981 tctggatggt actgacgctg tgatgctgtc ggcagaaact gctgctggtc aatacccgtc
     2041 agaaactgtt gcagccatgg cgcgcgtttg cctgggggcg gaaaaaatcc cgagcatcaa
     2101 cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcca ttgccatgtc
     2161 agcaatgtac gcggctaacc acctgaaagg cgttacggcg atcatcacca tgaccgaatc
     2221 gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctgccaattt tcgccatgtc
     2281 acgacatgaa cgtacgctga acctgactgc tctctatcgc ggcgttacgc cggtgcactt
     2341 tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc tgcgtgataa
     2401 aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt
     2461 gggttctact aataccacgc gtattttaac ggtagagtaa gtacgttg
//
LOCUS       DAOFSV010000287         2502 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.288, whole genome shotgun sequence.
ACCESSION   DAOFSV010000287 DAOFSV010000000
VERSION     DAOFSV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.288"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..891
                     /gene="psuG"
                     /locus_tag="QBH27_004767"
     CDS             <1..891
                     /gene="psuG"
                     /locus_tag="QBH27_004767"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDQ7016965.1"
                     /translation="VQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGA
                     VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMI
                     IAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTE
                     YLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARAMAVKWQTGLNGGLVVA
                     NPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQL
                     VFNNAILASEIAKEYQRLVG"
     gene            985..2235
                     /locus_tag="QBH27_004768"
     CDS             985..2235
                     /locus_tag="QBH27_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDQ7016966.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2302..>2502)
                     /locus_tag="QBH27_004769"
     CDS             complement(2302..>2502)
                     /locus_tag="QBH27_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="activator of nucleoside metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDQ7016967.1"
                     /translation="LYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKN
                     RKQLSGLALEMDPENKFSGMMQ"
BASE COUNT          589 a          551 c          660 g          702 t
ORIGIN      
        1 gtacaggatg ctttaaaaaa taaaaaaccg gttgttgcgc tggaatcgac cataatttct
       61 cacggaatgc cattcccaca aaatgcccaa accgcaattg aagtagaaga aacaattcgt
      121 aaacagggcg cagtaccagc cactatcgcc attattggcg gtgtgatgaa agtgggctta
      181 agtaaagaag aaatcgaatt gctgggccgt gaaggacata acgtgacgaa agttagtcgt
      241 cgtgatttac cttttgtcgt tgctgcaggg aaaaatggcg cgactaccgt tgcatcaacc
      301 atgattattg cagcactggc tgggattaaa gtttttgcaa ccggaggtat tggtggtgtg
      361 catcgcggag cggaacatac cttcgatatt tctgccgatt tacaggaact ggcaaatacc
      421 aatgtcactg ttgtttgtgc gggggcaaaa tctattctcg atttaggatt aacaacagaa
      481 tatttagaaa ccttcggtgt accgttaatt ggttatcaga caaaagcgct gcctgcgttt
      541 ttctgccgaa ccagcccatt tgatgtcagc attcgtctcg acagtgcccg cgaaattgct
      601 cgcgcgatgg cagtgaagtg gcaaaccggt ttgaacggcg gcctggtggt agcgaacccg
      661 atcccggaac agtttgcgat gccagaagaa accatcaatg cagcaattga tcaagccgtt
      721 gctgaagccg aagaacaggg tgttattggg aaagagagta caccgttcct gctggcgcgt
      781 gttgcggagc taaccggcgg tgatagcctg aaatccaata ttcaactggt ctttaataac
      841 gccattctgg cgagcgaaat tgccaaagaa tatcagcgtc tcgtgggtta atttctggtg
      901 ggcagaaagc cgtcgcccgt actcatcaaa cataccaaaa cctggcgctg acgccgggtt
      961 tactggcatg aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggta
     1021 ttgctggcaa ttgcctttgt gttgtcggta aataaaaaga gcatcagctt gcgcacggtt
     1081 ggggctgcgc tgctgctgca aattgccatt ggtggcatca tgctctattt tcccccggga
     1141 aaatgggcag tagagcaggc ggcattaggc gtccataaag tgatgtccta cagtgacgca
     1201 ggaagtgcct ttatttttgg ttcgctggtc ggtcccaaaa tggatgttct gtttgacggt
     1261 gcgggcttta tcttcgcctt tcgcgtactc ccggcgatta ttttcgttac cgcgcttatc
     1321 agcttgctgt actacattgg cgtaatgggg ctgctgattc gtattcttgg cagtattttt
     1381 cagaaagccc tcaacatcag caaaatcgaa tcttttgtcg cagtcaccac tatcttcctc
     1441 gggcaaaatg agatcccggc gatcgttaaa ccttttattg atcgcatgaa tcgcaacgag
     1501 ctatttacgg ctatttgtag cggcatggcg tctattgcgg gttcgatgat gattggctat
     1561 gccggaatgg gcgtgccgat tgactatttg ttagccgctt cgctgatggc tattcctggc
     1621 ggtatcctgt ttgcacgtat tcttagtccg gcgacggaac cttcgcaagt tacgtttgaa
     1681 aatctgtcgt ttagcgaaac accgccaaaa agcattattg aagctgcggc gaacggtgca
     1741 atgaccgggc tgaaaatcgc cgccggtgtg gcgacggtgg taatggcatt tgtggcgatc
     1801 attgcgctga tcaacggcat tattggtgga gttggcggct ggtttggttt tgccaatgtc
     1861 tctctggaaa gcattttcgg ctatgtgctg gccccgttgg catggattat gggggtggac
     1921 tggagtgatg caaatcttgc cggaagtttg attgggcaga aattggcgat taatgagttt
     1981 gttgcttatc tcaatttctc gccctatctg caaacgagcg gcacgctgga tgtcaagacc
     2041 attgccatta tctcgttcgc cctctgtggg tttgctaact ttggttctat cggtgttgtc
     2101 gttggagcgt tttcggctat ttcgccaaaa cgcgcgccgg aaatcgccca gcttggttta
     2161 cgggcgctgg cggcggcgac gctttccaac ctgatgagcg cgaccattgc cgggttcttt
     2221 attggtttag cttgagtttc attgccggat gtacgcctta ttaagcgttg catccggcaa
     2281 tcgcactatt gccaataatt tttactgcat catcccggag aatttattct ccgggtccat
     2341 ctccagcgcc agtcctgaca actgctttct gtttttaatg agataccctt tcttgctttt
     2401 aattaataaa ccgtcattaa tgaattgcgc gagaacatat aaaagatgtc gataagaaac
     2461 gcctaaatac tctgccgctt gcgtatgctt ttcgtgataa ag
//
LOCUS       DAOFSV010000288         2500 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.289, whole genome shotgun sequence.
ACCESSION   DAOFSV010000288 DAOFSV010000000
VERSION     DAOFSV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.289"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..391)
                     /locus_tag="QBH27_004770"
     CDS             complement(<1..391)
                     /locus_tag="QBH27_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HDQ7016968.1"
                     /translation="MEFNIKLLFVITSMVLNFNAAAKEKISLLAPYDEWYFNFFYPNS
                     LPAEVTYVELLDTDGIYYQFRTLASTNASPGSVGQWSEKVSGMSSVFNKAKNPPMSIH
                     FCWDSVIDKKVYETWITLAGDVWKLMLT"
     gene            complement(381..2384)
                     /gene="hcp"
                     /locus_tag="QBH27_004771"
     CDS             complement(381..2384)
                     /gene="hcp"
                     /locus_tag="QBH27_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDQ7016969.1"
                     /translation="MGDIIYLRITGEQQGDISAGCGTQVSVGNRYQQGHEDEIFVFSF
                     QGGVSNTGFGINHQAIQFCKILDKSSPLLMNCINNNERCRFEFYFYRINKYGKWERYY
                     YIEVRGATLTQNQIIIKENELDYQYITIHYEYIYCKHLTANTEFSYLLTPENYNRLFP
                     PTLLPVEEKPEIPPEREIILTIGVFFDGTGNNLTNTNLRMSFCQPETYGLDVQDLASF
                     NKQCMSKQGKTGSGVQSYLNYYTNIHWLNKLYHRQLVLDDDVFNIQEKIYIEGIGTEN
                     NKADSLVGMGLGNNDTGVIAKTDRAISLLRETLAKIIVDLKDAKLVIKRLQFDVFGFS
                     RGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPSGEVQFLGIFDTVAAIGGLMD
                     GFDPHDGNNFPVKVKLPPGVAKHVFQLTAMNECRYNFCLNSIKESWPELSFPGSHADI
                     GGGYDPLEDEYLFLSRPAIETVLGNVPVEATSAYQKAAQQAELLWRHSTLAPILPSGV
                     LKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWSKIALRVMYEVAKEAGVVFDA
                     ISVADKELKVPSHLKVICEKAISQGKAVLLCTAPMPFSDDELKTIGKYLHCSSNWNTV
                     DYRLKNKVSAEVSAFKTFSFVNRPDENWTRTIYDMQGNKQNGI"
BASE COUNT          725 a          545 c          434 g          796 t
ORIGIN      
        1 gtgtgagcat caatttccag acgtctcccg ccagtgttat ccaggtttca tacacttttt
       61 tatcaatcac tgaatcccag caaaaatgga tagacattgg tgggtttttg gctttattaa
      121 aaactgacga catccctgat actttctcgc tccattgtcc aacacttcct ggactggcat
      181 tagtactcgc cagagttctg aactgataat agatgccatc ggtatctaat aactccacat
      241 aggtaacttc tgctggtagg gaattagggt aaaaaaaatt gaaataccat tcatcatagg
      301 gtgcaagcag tgatattttt tctttcgctg ctgcgttaaa gttaagaacc atagacgtaa
      361 taacaaacag taattttata ttaaattcca ttttgtttat tcccttgcat atcgtatata
      421 gttctggtcc aattttcatc cggccgatta acaaaactaa acgtcttaaa cgctgatact
      481 tcagcagaca ccttattttt taatcgataa tcgacggtat tccagttgga agagcagtgt
      541 aaatatttcc caatggtttt aagctcatca tcagaaaaag gcatcggcgc tgtacacaag
      601 agaacggctt taccctgaga aattgctttt tcacatatta cctttaaatg actgggaact
      661 tttaattcct tatcagcaac agaaatggca tcaaatacta cccctgcctc tttagcgact
      721 tcatacatta ctcttaacgc aatctttgac cagtcattgg tgatcgttct gcgaaatgtc
      781 accgctgcag cgacccgttt tctcacatta cctaagtggt caggcgacgc tatttcatca
      841 atattggatt caatttttag gacaccagaa ggcagaatcg gagccagcgt tgaatggcgc
      901 cacaataact ctgcctgctg tgctgctttt tggtaagcgc ttgtcgcttc caccggcaca
      961 tttcccagta ccgtttcaat cgccgggcga cttaaaaaca ggtactcatc ttccagtggg
     1021 tcatagccgc caccaatatc agcatgtgat ccagggaaac ttaattctgg ccagctttcc
     1081 tttatgctgt tgaggcaaaa gttatagcga cattcgttca ttgctgtgag ctggaataca
     1141 tgtttggcca cacctggcgg taacttcact tttaccggaa agttattacc atcatgaggg
     1201 tcaaaaccat ccatcagccc tccgatggct gcaactgtgt caaaaatacc taaaaattgc
     1261 acttccccgg aaggtttacc taaatattca actgactgaa atgccgtcgc aatggtttta
     1321 atcagtacgg gatcacgttc aaatacgcga ttagtaaagt gccgtgcggc tgccgcccca
     1381 cgactaaagc caaatacgtc aaactgtaga cgcttaatta ctagtttagc atcctttaaa
     1441 tcaacaataa tctttgccag tgtctcacga agcagtgata tcgcacggtc agttttggca
     1501 attaccccag tatcattatt tcctagcccc attcctacca gagagtctgc tttattattt
     1561 tcagtgccaa taccttcaat atatattttt tcctgaatat taaatacatc atcatctaaa
     1621 actaattgcc gatgatacaa cttattcaac caatgaatat tcgtatagta attcaaatag
     1681 ctctgtactc cggatcccgt tttaccctgc ttgctcatac attgtttatt aaacgaggcc
     1741 aaatcctgga catccagacc ataggtttct ggttggcaaa aactcatacg taaattcgta
     1801 ttggttaaat tgtttcctgt gccatcaaag aatacgccaa tggtgaggat aatttctcgt
     1861 tcaggcggta tttctggttt ttcctcgaca ggtaagagcg tcggtgggaa aaggcgatta
     1921 taattttcag gtgtgagtaa ataactaaat tctgtattgg ctgtaagatg cttacagtag
     1981 atatattcgt agtgtattgt tatatactga tagtcgagtt cattttcttt tattataatc
     2041 tgattctgag ttaacgtggc accacggact tcaatataat aatacctttc ccattttcca
     2101 tatttattaa tacgataaaa ataaaactca aaacggcatc gttcgttatt attaatgcaa
     2161 ttcatcagca gcggtgagct tttatcgaga attttacaaa attgtatcgc ttgatgatta
     2221 atgccaaatc ctgtgttact tactcctccc tgaaaactaa aaacaaatat ttcatcctca
     2281 tgtccctgtt gataccgatt cccgacagag acttgcgtcc cgcatcctgc agaaatatcc
     2341 ccttgttgct cccctgtaat gcgtaaatat ataatgtcac ccatatgatt atacccattt
     2401 catagatttt aattatataa tataaactta ataaacatct catttagtaa tgtatattaa
     2461 ttatatgttt gggcataaca tttttttaat tacgtgatgc
//
LOCUS       DAOFSV010000289         2466 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.290, whole genome shotgun sequence.
ACCESSION   DAOFSV010000289 DAOFSV010000000
VERSION     DAOFSV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.290"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(27..881)
                     /gene="rclR"
                     /locus_tag="QBH27_004772"
     CDS             complement(27..881)
                     /gene="rclR"
                     /locus_tag="QBH27_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDQ7016970.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1108..2433
                     /locus_tag="QBH27_004773"
     CDS             1108..2433
                     /locus_tag="QBH27_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDQ7016971.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          693 a          553 c          577 g          643 t
ORIGIN      
        1 tttgccctga tttctggcgg ttttattcag ggtgcaagct gtctcaccct ttcccgatat
       61 tctcccggag tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga ttcactggca
      121 tagcctactg actcagcgat caccacaaca gggagcattt cccgggaaaa catctgggcc
      181 gctatttgta gacgcaactt tgttaataca gccagcggcg tggttccgga aacatcacgg
      241 aaaagctggg caaaacttgc ccgggacatg tgggcgatgc tggccagcga ttcgacggtc
      301 caggcgtgtc caggcatttc catcatttgc tgtattaccg caccaagacg tggatgcaga
      361 agcaaactga gaatgttttt ctctgtatta acctgtgcaa tccactcacg caccgcgagg
      421 gtaaagaatg tagcgcagat ctggctacac agagcatcca ctcccggcat tgccgatctg
      481 gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt tatattccac gctatggttc
      541 actggtgcta caaacagcgt ttccggcaaa gacgttaaaa aataacgcgc cgaatgttgc
      601 agccgaagag ttccacagac aatacaggtc gattcattat cgacatgact caggcgatga
      661 gctgaatttt gtggtagcag gaccacattt cccgggcgta atgtaaaaat ctctcccgtc
      721 ggcatttcca gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac cgacaactcc
      781 ccggcaccat gtggaagttg ccagtcactt cctaacacgc aattcttatc gatcgttcct
      841 tgtggagcgt taagcatcaa caaacggcta agagcatcca tatcagactc ctgagtggtt
      901 tttggattac aaacaccaac aatcttagtc aataattaat ctaataccat caaatagtta
      961 atcatgattt ttgttttgtg attctgatta agaaaaagaa aataccagac gataagacaa
     1021 aatatcaagc gttgtgaaga aatgttattt gctcttttgc gtctatagtc atgatgtcaa
     1081 atgaacgcgt ttcgacagga aatcatcatg aataaatatc aggcagttat tattggtttt
     1141 ggcaaggctg gaaaaacatt agccgtcacg ctggcaaaag caggttggcg tgtggctctc
     1201 atcgaacaat caaatgcaat gtatggcggg acctgtatta atatcggctg tatcccaacc
     1261 aaaacattgg ttcatgacgc acagcagcac acagattttg tccgtgccat acagcgtaaa
     1321 aatgaagtgg ttaatttttt acgtaataag aattttcata atcttgcgga tatgcccaat
     1381 atcgacgtga tcgacggcca ggcggagttt atcaataatc atagcctgcg tgttcatcgg
     1441 cctgagggaa atctggaaat tcatggcgag aaaattttta ttaataccgg tgcacaaacc
     1501 gtggttccgc caattcctgg aattaccacc acgccaggag tatatgacag caccggatta
     1561 cttaatctaa aagaattgcc tgggcattta ggtattttgg gcggcggata tattggcgtt
     1621 gagttcgcct ctatgttcgc taattttggc agcaaagtaa ccattttaga ggcagcttcg
     1681 ctgtttttgc ctcgggaaga tcgggatatt gctgataata tcgcgacgat tttacgcgat
     1741 cagggcgtcg atataatcct caatgcccat gtggagcgaa tcagtcacca tgaaaatcaa
     1801 gtgcaagtgc atagcgaaca cgcccaactg gcggtggatg cactgttaat agcttccggt
     1861 cgtcaaccgg ctaccgcttc gttacatcca gaaaatgcag gtatcgcagt aaacgaacgc
     1921 ggggcaattg tcgttgacaa gcaattacat accaccgcag acaatatttg ggcgatggga
     1981 gatgttaccg gcgggctgca atttacttat atttcactgg atgattaccg cattgtgcgt
     2041 gatgagttgc tgggagaagg cagacgtagt actgatgatc ggaaaaatgt gccttattcc
     2101 gtattcatga caccgccact gtccagggtt ggtatgacag aagaacaggc cagagagagt
     2161 ggtgctgata ttcaggtggt gacattgcct gtagctgcaa ttccgcgtgc cagagtgatg
     2221 aatgatactc gtggggtatt aaaagcgatt gttgataata aaacccaacg tatattaggg
     2281 gcatcactgc tgtgtgttga ctcccacgag atgatcaata tagtgaaaat ggtgatggat
     2341 gccgggctgc cttatagcat attacgcgat cagatattta ctcatccgtc gatgagcgaa
     2401 tcactcaatg atctattttc attagtcaaa taaactcaaa atcagacgcc agaacaaata
     2461 ttctgg
//
LOCUS       DAOFSV010000290         2457 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.291, whole genome shotgun sequence.
ACCESSION   DAOFSV010000290 DAOFSV010000000
VERSION     DAOFSV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.291"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(206..1579)
                     /gene="cobA"
                     /locus_tag="QBH27_004774"
     CDS             complement(206..1579)
                     /gene="cobA"
                     /locus_tag="QBH27_004774"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDQ7016972.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1598..2404)
                     /gene="nirC"
                     /locus_tag="QBH27_004775"
     CDS             complement(1598..2404)
                     /gene="nirC"
                     /locus_tag="QBH27_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDQ7016973.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
BASE COUNT          644 a          672 c          602 g          539 t
ORIGIN      
        1 tgaatataag cagactgctc attaagaacg atacaggaat attcaattag cgtcagtgag
       61 cgattggctt aattttgtaa gttgctgtaa cggtgaatat aacccattag cagacaatat
      121 ttattaaggg gttatatttt tatctgctga atatataaag agagttgtaa acgaaaaagg
      181 ccagacttct ctggccctgg ttaaattaat ggttagagaa ccagttcaat ttatcgcgca
      241 ggccaacaac ccgaccaata ataattagcg atggactgtt catttgctgc gccagttcgc
      301 ccagctgcgt gagcgtaccg tcaatcacgc gctgcgtgac tgccgttccg ttttcgacaa
      361 ttgctaccgg catttcacca ggcataccgt attcaatcag cttttgctga atagttgcag
      421 cctgattcag ccccatataa aacaccagcg tctgtttttc tgccgccagg ttttcccagt
      481 ccagctcgcc accggttttt aagtgtccag taattaagcg tacgctctgg gcataatcgc
      541 gatgcgtgag cggaataccc gaataggcag agcaaccaga ggctgcggta atacccggaa
      601 ccaccgagaa tggaataccc gcgttgcaca gtgtttccag ctcttcgcca ccacggccaa
      661 aaataaacgg atcgccgcct ttcagccgca ccacgcgttt gcctttttgc gcttcccgca
      721 gcaggatctg gttaatctct tcctgcggta cgcagtggta tcccgcgcgt ttgccgacga
      781 aaacgcgatc agcatcgcgg cgtaccagat tcataatatc gtcagaaacc agacggtcgt
      841 agaccaccac atctgcctgc tgaatttgtt gcagaccttt cagtgtcagc agcccggcat
      901 cgcccggacc tgcaccaacc agcaccactt caccgcgatg atcgagcggt tcattgatta
      961 actgttcggt ggtttcagta atggctttct gatcgttgtt ggccagcgac tgcgccaggc
     1021 ggtcattaac gaacaatttc tcccagaaac ggcgacgctc acccatcgtg gcgaactgtt
     1081 gtttcactcg cccgcgtaat tgcccggcgt atttcgctac ctggcccaga tgtaacggca
     1141 gcagtgattc gagtttttcg cgcaacaggc gcgccagaac cggagaggtg ccgccagagg
     1201 agaccgctac catgagcggc gagcggtcaa taatcgacgg cataataaag ctggcggctt
     1261 tcggcgcatc gaccacgtta cagaagatgc gacgagcttc agcggcttcg ctgacgcgct
     1321 ggttaagcgc gtcatcatcc gtcgctgcaa tcgccagcca gcaggtgtcg agaaggcttt
     1381 catcaaatgg cccttcgacg agggttaaca tgcctgcatc tgcccatgcg gtgaactgtg
     1441 gaataaacgc taatgcattc accgttaagc gagcgcctgc gtctaacagc aaccttgctt
     1501 tgcgttccgc gacatcacca ccgccgacaa tcagacagtc gcgatcgcgt aattggcaaa
     1561 atataggcaa atgatccacg taaaaacccc ttaataatta accggcagcc gtttcagttt
     1621 gattaaattt gtccgcaacc ggacgattcg ctttcggcgt agcataccaa taacccaatc
     1681 ccatgaatac ggcacccgat aaagtattac ccagcgtcac ccacagcagg ttatgaccaa
     1741 tacccgccag cgtgtaggct tcgctgtggt tgccgaacca ggagagggcg aacagcgtca
     1801 tgttagcgat ggagtgctcg tagccggacg cgataaatgc cagcaaacac caccagatag
     1861 cgataaattt cgccgcccct tcagtgcgca gcgccatcca gattgccagg caaaccagcc
     1921 agttacacaa tgcacctttg aagaacagta ccattgccgg tgcagtggtt ttagccagcg
     1981 cgacggagtg aacgatgctg gtatcgaccg gcagcaggct accgccaccc cagctataga
     2041 gcatggcaac aaagacggaa ccgaccaggt tacccagcca ggtttgcggc aggattgccc
     2101 acatttgccc gtggctgata gtgcccgctt tcactccaaa ggtgaggaac atggtgtgtc
     2161 cggtgaacag ttcagaaccg gcgataatca ccagcgttaa ggcgatacca aaggtcgcac
     2221 ccatcaccag aggacgtacg gacgggtcga gcaaattacc gagcgtgaaa atcaggatga
     2281 tcccaagacc cacatacgcg cccgccatgg cggagctgac ccaaaagccg agcgggttat
     2341 ttgccgacag gcgtgcaatg cgcgcagcgt tagccgcaca cttagtaata gtgtctgtaa
     2401 acatttgatt atcctttaaa aaataaaaac aaaaaataaa acaaaaagtt taaaaaa
//
LOCUS       DAOFSV010000291         2440 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.292, whole genome shotgun sequence.
ACCESSION   DAOFSV010000291 DAOFSV010000000
VERSION     DAOFSV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.292"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            53..478
                     /locus_tag="QBH27_004776"
     CDS             53..478
                     /locus_tag="QBH27_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDQ7016974.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            475..825
                     /gene="tnpB"
                     /locus_tag="QBH27_004777"
     CDS             475..825
                     /gene="tnpB"
                     /locus_tag="QBH27_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDQ7016975.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            856..>2440
                     /locus_tag="QBH27_004778"
     CDS             856..>2440
                     /locus_tag="QBH27_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HDQ7016976.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELL"
BASE COUNT          620 a          609 c          689 g          522 t
ORIGIN      
        1 agacgagaat tggtaactta gacgcccatc tgatatagac ggacatctaa gtatggaatt
       61 acaggactgg cgaaaagaac ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt
      121 ggaactggca tcacaacctg gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
      181 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg
      241 tcttccggta acgacctctt ctgacactgg cgttgaatta ttacctgtag aaataacgcc
      301 ggatgagcag aaagaacctg tggcggccat tgcgccgtct ttatccactt ccactcagac
      361 cagagtcagt gccagttcct gcaaggtgga attccgtcac ggtaacatga cgctggaaaa
      421 tccatcgcca gagctgctca cagtgttgat ccgtgaactg accgggaggg gaagatgatc
      481 tcactcccat caggtacccg tatctggctc gttgccggcg ttaccgatat gcgtaaatcc
      541 ttcaacggac tgggagaaca ggtacaacat gtgctgaatg ataatccctt ctccggtcac
      601 ctgtttatct tccgtggccg acggggtgac accgtcaaaa ttctttgggc tgatgctgat
      661 ggtctgtgcc tgttcaccaa acgcctggag gaaggccagt ttatctggcc tgcggtacgt
      721 gacggcaagg tatccattac ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg
      781 cgtcagccaa aaacatccag ccgtaactca ctgacaatgt tgtaaaaaac tcctgaccgc
      841 attataaaaa cggtcatgag tcagaaatac ctcattcgca tcgcagagct ggaaaggttg
      901 ctctctgagc aggctgaagc cctccgtcag aaagaccagc aactgagtct ggttgaagag
      961 acggaagcct tcctgcgctc tgcactgaca cgtgccgaag aaaagatcga agaagatgaa
     1021 cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct gttcggtacc
     1081 cgttctgaaa aactgcgtcg tgaagttgaa ctggctgagg ctctgctgaa acaacgtgaa
     1141 caggacagcg atcgttacag tgggcgggaa gacgatcctc aggttccccg ccagttgcga
     1201 cagtcgcgcc atcgtcgtcc gttaccggca caccttcccc gtgaaataca ccgcctggag
     1261 ccagaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaagtcagc
     1321 gctgaacagc tggaactggt gagcagtgcc ctgaaagtga tccgcacaga acgggtaaaa
     1381 aaagcctgta caaaatgtga ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag
     1441 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacat
     1501 ctgccactgt atcgtcagag tgaaatcttt gcccgccagg gtgtcgaact gagccgggcc
     1561 ttactctcca actgggttga cgcgtgctgc cagttaatga caccggtgaa tgatgccctg
     1621 taccgttatg taatgaacac ccgcaaggtt cacactgatg acacaccggt aaaggtactg
     1681 gcaccgggtc agaaaaaggc gaaaacaggg cgtatctgga cgtatgtccg ggatgatcgc
     1741 aatgtgggtt cgtcatctcc tccagcggtc tggttcgcgt actcgccgaa ccggcagggg
     1801 aaacacccgg agcaacacct ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca
     1861 ggttacgaca ggttgttcag tgcagaacgt gaaggtggtg cactgacaga agttgcgtgc
     1921 tgggcccatg cccggcgaaa aatccacgat gtatacatca gcagcaaaag tgcgacggca
     1981 gaagaagccc tgaagcgaat cagtgaactg tacgccatcg aggatgaaat acggggatta
     2041 ccggagtcag agcgtcttgc cgtcaggcag cagcgaagca aagtgttact gacgtcgctg
     2101 catgaatgga tggtggagaa gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg
     2161 ttcagctatg tactgaatca gtgggatgcc ctctgttatt acagtgatga cggtctggcg
     2221 gaggcggata ataatgctgc ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac
     2281 tttatgttct ttggcagcga tcacggcggc gagcgtggag cactgttgta cgggctgatc
     2341 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat cctgagcgta
     2401 ctgccggaat ggccttccaa ccgagttgac gaactcctgc
//
LOCUS       DAOFSV010000292         2440 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.293, whole genome shotgun sequence.
ACCESSION   DAOFSV010000292 DAOFSV010000000
VERSION     DAOFSV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.293"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            72..500
                     /gene="rplK"
                     /locus_tag="QBH27_004779"
     CDS             72..500
                     /gene="rplK"
                     /locus_tag="QBH27_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDQ7016977.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            504..1208
                     /gene="rplA"
                     /locus_tag="QBH27_004780"
     CDS             504..1208
                     /gene="rplA"
                     /locus_tag="QBH27_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDQ7016978.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            1500..1997
                     /gene="rplJ"
                     /locus_tag="QBH27_004781"
     CDS             1500..1997
                     /gene="rplJ"
                     /locus_tag="QBH27_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDQ7016979.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            2064..2429
                     /gene="rplL"
                     /locus_tag="QBH27_004782"
     CDS             2064..2429
                     /gene="rplL"
                     /locus_tag="QBH27_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDQ7016980.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
BASE COUNT          658 a          571 c          635 g          576 t
ORIGIN      
        1 gcccgtaaaa cgatttttta tatcacgggg agcctctcag aggcgttatt acccaacttg
       61 aggaatttat aatggctaag aaagtacaag cctatgtcaa gctgcaggtt gcagctggta
      121 tggctaaccc gagtccgcca gtaggtccgg ctctgggtca gcagggcgta aacatcatgg
      181 aattctgcaa agcgttcaac gcaaaaactg attccatcga aaaaggtctg ccgattccgg
      241 tagtaatcac tgtttacgct gaccgttctt tcactttcgt taccaagact ccgccggcag
      301 cagttctgct gaaaaaagcg gctggtatca agtctggttc cggtaagccg aacaaagaca
      361 aagtgggtaa aatttcccgc gctcaactgc aggaaatcgc gcagaccaaa gctgccgaca
      421 tgactggtgc cgacattgaa gcgatgactc gctccatcga aggtactgca cgttccatgg
      481 gcctggtagt ggaggactaa gaaatggcta aactgaccaa gcgcatgcgt gttatccgcg
      541 agaaagttga tgcaaccaaa cagtacgaca tcaacgaagc tatcgctctg ctgaaagagc
      601 tggcgactgc taaattcgta gaaagcgtgg acgtagctgt taacctcggc atcgacgctc
      661 gtaaatctga ccagaacgta cgtggtgcaa ctgtactgcc gcacggtact ggccgttccg
      721 ttcgcgtagc cgtatttacc cagggtgcaa atgctgaagc tgctaaagct gcaggtgctg
      781 aactggtagg tatggaagat ctggctgaac agatcaagaa aggcgaaatg aactttgacg
      841 ttgttattgc ttctccggat gcaatgcgcg ttgttggcca gctgggccag gttctgggtc
      901 cgcgcggcct gatgccaaac ccgaaagtgg gtactgtaac gccgaacgtt gctgaagcgg
      961 ttaaaaacgc taaagctggc cagattcgtt accgtaacga caaaaacggc atcatccaca
     1021 ccaccatcgg taaagtagac tttgacgctg acaaactgaa agaaaacctg gaagctctgc
     1081 tggttgcgct gaaaaaagca aaaccgactc aggcgaaagg cgtgtacatc aagaaagtta
     1141 gcatctctac caccatgggt gcaggtgttg cagttgacca ggctggcctg agcgcttctg
     1201 taaactaatg cctttacgtg ggcggtgatt ttgtctacaa tcttaccccc acgtacgcta
     1261 acgaaagtta gcagctaaaa agatttgttc gttggagcct ggcctatcca ggcctccgtc
     1321 gaagaccgca ggagtttcgc aagaaactta atcccctgcg tagacggtga cagaacgcta
     1381 agattattct tttatattct ggcttgtttc tgctcaccgt aattaagacg ctctctccgt
     1441 ttggaggagt gaagtgagtt ccagagattt tctctggcaa acatccagga gcaaagctaa
     1501 tggctttaaa tcttcaagac aaacaagcga ttgttgctga agtcagcgaa gtagccaaag
     1561 gcgcgctgtc tgcagtagtt gcggattccc gtggcgtaac tgtagataaa atgactgaac
     1621 tgcgtaaagc aggtcgcgaa gctggcgtat acatgcgtgt tgttcgtaac accctgctgc
     1681 gccgtgctgt tgaaggtact ccgttcgagt gcctgaaaga cgcgtttgtt ggtccgaccc
     1741 tgattgcata ctctatggaa cacccgggcg ctgctgctcg tctgttcaaa gagttcgcga
     1801 aagcgaatgc aaaatttgag gtcaaagccg ctgcctttga aggtgagctg atcccggcgt
     1861 ctcagatcga ccgcctggca actctgccga cctacgaaga agcaattgca cgcctgatgg
     1921 caaccatgaa agaagcttcg gctggcaaac tggttcgtac tctggctgct gtacgcgatg
     1981 cgaaagaagc tgcttaatcg cagttatctt tttaacgcat tcgcttacgt ataaacttat
     2041 tctgatattc aggaacaatt taaatgtcta tcactaaaga tcaaatcatt gaagcagttg
     2101 cagctatgtc tgtaatggac gttgtagaac tgatctctgc aatggaagaa aaattcggtg
     2161 tttccgctgc tgctgctgta gctgtagctg ctggcccggt tgaagctgct gaagaaaaaa
     2221 ctgaattcga cgtaattctg aaagctgctg gcgctaacaa agttgctgtt atcaaagcag
     2281 tacgtggcgc aactggcctg ggtctgaaag aagctaaaga cctggttgaa tctgcaccgg
     2341 ctgctctgaa agaaggcgtg agcaaagacg acgcagaagc actgaaaaaa gctctggaag
     2401 aagctggcgc tgaagttgaa gttaaataag ccaacccttc
//
LOCUS       DAOFSV010000293         2422 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.294, whole genome shotgun sequence.
ACCESSION   DAOFSV010000293 DAOFSV010000000
VERSION     DAOFSV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.294"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..200)
                     /locus_tag="QBH27_004783"
     CDS             complement(<1..200)
                     /locus_tag="QBH27_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDQ7016981.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKA"
     gene            complement(351..2204)
                     /locus_tag="QBH27_004784"
     CDS             complement(351..2204)
                     /locus_tag="QBH27_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HDQ7016982.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNATYGVEIESLVLEINAPASS"
BASE COUNT          601 a          674 c          553 g          594 t
ORIGIN      
        1 gccttacgac ggtcctctct gattttgaaa tacaggttag tcagatatgt cagcagccca
       61 aacagcagac tccccagcac gcctattgcc gcccactgag acggggaaac cctgtccagc
      121 aactgcagga accagtagcc cgttcccacc gctgacgtgg tgtatgacac acctgttgtg
      181 attttttcca tctggtacat accccgtctc ccgttatccg gaagctgaca acaataaaaa
      241 aagccaccag ttaagtactg atggctctga taactcatgc aggcatctca gacgacccac
      301 tgacactacc ggtgagttta acgatacctt ccatttgact ggctcacttt ttatgatgat
      361 gccggtgcat ttatctccag caccagactt tctatctcaa cgccatacgt tgcatttttg
      421 gtaatatccg tcagcgtcag cgcattcagc cccagtgtca gactgtcttt tatgacctgg
      481 aatgccgggc cagccactcc attcagtttc ggagtaaccg tggcactgcc ggcggtgaac
      541 accagctcca gcgtctgcca gtcgttactg taattcccga actcgcccaa ctttgtgttt
      601 cctgctttct tgtgatgcat cagattcagt ttgccgtctg tggtctgggt gaagaacgac
      661 atcaggaacg ggttaccagt cccggtcatc gccacgacgt caggtaacgc tacatcggta
      721 tacagataaa ttcccagacc gaactgattg ttggtcagtg cgcctgacag tcgaaactta
      781 cagctcagtc tgccaccccg tgtcagcagg gagactgcgt catccaccgg gcgcgtcagg
      841 gaccaggctt tattgctctg cttggtgatc ttaaatacac catctgacaa ctgaattccg
      901 ccattcttaa tgctccagcc ctgcgcagca gcatctccgg ctgtcggcaa cagggagatt
      961 gtgcgtacgg atgcatcttc agacggcccc gatggcgtgt cgccgccggg cgagggtttg
     1021 atttccggtg ccttaccact aatgaaggct aaggtgcgac cggctacgtt cagaatagca
     1081 gttgccatac gatcgggaat aatgccacga cgcgcccatg agctgaaatg cgtcgggcga
     1141 tttgatgata cccagttttt gttcgttcgg gatgccgaac cgtaataacc agacccggca
     1201 atatcaggat cttctgacgg gttgtttgtc ggtgtattaa ctccgctacc atcggtcata
     1261 aagggaacaa aataaatctg ctgggattct ttacctttat atgcaccata taccacttca
     1321 tattgcgtac cgtgttcttg tttccacgcg tatgtcgtgt cgccacaaat ccaggggact
     1381 gatgccggac ttccaccgtg acactgcgcc gccagcccgg caaggtcagc acggaactgc
     1441 tgtaccattg caagaaatgc tgctggctgc tgggcgtaac tggcattcgt catatcgaat
     1501 tccccctgca tccagcatat cgccagcaaa acgtttttcg ggtttttctg caatgctgcc
     1561 ttcgtgcgga aaagcagatc ctgatataac ggcttaccca ctccccagcg agccgaatcc
     1621 tgactggccc ccgtggactc gctgaatgtc ccctccgtgc cctgggtgaa tgccgaacca
     1681 ccacgacagc atggtaccag caggatcccc gcattattag ggatatacgg aagcagtttt
     1741 ttggcaatat gtaagccctg tccgacacag ccgtactgcc ctttgctcag gtcagcccgg
     1801 ggatggttaa tcgtactcat atcctgaaca tcatgcagac aatggtcagc aggaatgatg
     1861 tcgttaaata cgcatacttc accaccggga gtcactgtgt tacgacgggc cagttgctta
     1921 atgcgcggat ggggcgcatc gtatgaatcc ggaagcggaa gcccttcacc gtaagccatg
     1981 gcattggatt gcccggccag tacgatgacg tagtaccact ccggctcagt tgcaccactg
     2041 acgaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt tatggccacc
     2101 ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc cgcaagatct
     2161 gacggcgacg ccgccctgac aacatcatag tgtttaaatg ccatggttct ttccaccatc
     2221 tgaaaaatga ttctttaaaa tacctgacat gtaatacaga aaaaacacaa aaccatacct
     2281 taaataaaaa cctcatcatc aagcagatat gcatggataa actacaagac gagatataaa
     2341 ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc tctatggttt
     2401 acaatcaaaa atatcattta ta
//
LOCUS       DAOFSV010000294         2418 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.295, whole genome shotgun sequence.
ACCESSION   DAOFSV010000294 DAOFSV010000000
VERSION     DAOFSV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.295"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            245..928
                     /locus_tag="QBH27_004785"
     CDS             245..928
                     /locus_tag="QBH27_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDQ7016983.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            929..1150
                     /locus_tag="QBH27_004786"
     CDS             929..1150
                     /locus_tag="QBH27_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016984.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            1164..1598
                     /locus_tag="QBH27_004787"
     CDS             1164..1598
                     /locus_tag="QBH27_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDQ7016985.1"
                     /translation="MYGTCETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            1649..>2418
                     /locus_tag="QBH27_004788"
     CDS             1649..>2418
                     /locus_tag="QBH27_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016986.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIR"
BASE COUNT          515 a          697 c          722 g          484 t
ORIGIN      
        1 gacgggcgct tttactgctc ccggctggca ccggtgacag tgtacgccag cctgtcgccc
       61 ttttctgatg aacgcccttc aagccgcttt cgcggcatat tctcgccgtc aaaaagacga
      121 cggctgcgat attccacggt cggcctgacc cgttaccgga cgcggtgaac agcccacagg
      181 cagcggggaa cgggcaccac aggggggccc tcccggtgcc ctctgtaaga gaaggagcgt
      241 tgttatgtcc cgttttgtcc ttggtaactg catcgatgtt atgacgcgta tccctgataa
      301 cgccattgat ttcatattaa ctgacccgcc atatctcgtc ggttttcgtg accgttccgg
      361 gcgcaccatc gccggcgata aaaccgatga atggctgcaa ccggcctgta atgaaatgta
      421 ccgcgtactc aaaaaagacg cgctgatggt gagcttctac ggctggaacc gcgtcgatcg
      481 ctttatggcc gcctggaaaa atgcgggatt cagcgttgtt ggtcacctgg tcttcaccaa
      541 aaactacaca tcgaaggccg catatgtggg ctatcgccac gaatgcgcct acatcctggc
      601 aaaaggccgt ccacgtctgc cacaaaaccc gctgccggac gtgctgggct ggaaatattc
      661 tggcaatcgt catcacccga cggaaaagcc cgttaccagc ctgcaaccgc tgattgagag
      721 cttcacacac ccgaacgcaa ttgtgctgga cccgtttgca ggcagcggct caacctgcgt
      781 cgccgccctc cagtccggac gccggtatat cggtatcgag ctgcttgagc agtatcaccg
      841 tgccgggcag caacgccttg ccgccgtgca acgggccatg cagcaggggg ccgcgaatga
      901 tgactggttt atgccggagg ctgcgtaaat gaactatgcc ggacacgaaa aacttcgcgc
      961 cgacgtggcg gaggtggcca acaccatgtg tgaccttcgg gccaggctga acgatatgga
     1021 gcatcgctgt cgctttgatt ccgatgtgct tgttgaacgc ctgacacgcc agacgctgta
     1081 tcgcgccaat cgcctgttta tggaggcgta taccgaaatt ctggaactgg attcctgctt
     1141 caaagattga gaaggagacg agaatgtacg gaacatgcga aacgctatgc cgggagctgg
     1201 cagcaaagta tccgggagac gcgccgctga tgctggttat ctggtccccg gaagagattc
     1261 aggcactggc tgacggtatg gaaatttctc tgaccgatca tgaaatcagg accgtcctgg
     1321 cgcgcatgga ggacatcccg gaagaccagc ggactgaatc cggtatttct tccgccgcgg
     1381 tgatggagat tatcagcaac gtgagcgaaa accgccaggt gaccgtccct gctgaactgc
     1441 tggcgtccct gattcagacc gctgaacagg cactgtggaa acgtgagtgg gccgcccgtg
     1501 ataacggcct cgccgtcccg gaatgcgtta cccgccgtca ggcggtggtt aatcaggccc
     1561 gcaccctgct gaaaaacaac acacacgaaa acaactgatg ttatcgccgc ctgccctgcg
     1621 ggcggcgata cagggagaac gcttaatcat gaacgaaaac agcacgctga acgcactgat
     1681 ttgtcgtcac gcccgtaacc tgctgctggc gcagggctgg ccggaagaga cggatgttga
     1741 ccagcgaaat ccgaactatc cgggctggat cagcatttac gtgcggctgg atgcgccccg
     1801 gctggcgacg ttacttatca accgtcacgg cggcgtactg ccgccgctcc tggcctccgc
     1861 cattcagaga ctgaccggaa ccggggcgga actggtactg tccggcagtc agtggcagtc
     1921 gctgccggta cttccggcag acggaacgca ggtgtctttc ccgtatgccg gagaatggct
     1981 gaccgaagac gaaatcaggg ctgttcttga tgcggtgcac gatgcggtac gaagcatctg
     2041 ttaccaggtg gcagaagatg cgcggcgtat ccgtgcggcg ctgaccacca ccggtcagac
     2101 gttgctgacc cgccagacgc gccgctttcg cctggtcgtg aaggaaagcg atcacccctg
     2161 ctggctcgat gaagatgacg aaaacctgcc cgtggtgctc gatgccatcg tgaaccgggg
     2221 agcacgtttt tcgtcggtgg aaatgtatct ggtcagtgag tgtattgagc atatcctgtc
     2281 cagtgggctg gcctgcgatg tgctgcgtat accggatgaa ccgccccgcc gctggtttga
     2341 ccgtggtgtt ctgcgggagg tggtccggga agcccggacc gaaatccgca gcatggcgga
     2401 tgccctggca aaaatccg
//
LOCUS       DAOFSV010000295         2309 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.296, whole genome shotgun sequence.
ACCESSION   DAOFSV010000295 DAOFSV010000000
VERSION     DAOFSV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.296"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1157)
                     /gene="icd"
                     /locus_tag="QBH27_004789"
     CDS             complement(<1..1157)
                     /gene="icd"
                     /locus_tag="QBH27_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDQ7016987.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINA"
     gene            1329..1982
                     /gene="rluE"
                     /locus_tag="QBH27_004790"
     CDS             1329..1982
                     /gene="rluE"
                     /locus_tag="QBH27_004790"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDQ7016988.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            1992..>2309
                     /gene="nudJ"
                     /locus_tag="QBH27_004791"
     CDS             1992..>2309
                     /gene="nudJ"
                     /locus_tag="QBH27_004791"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDQ7016989.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRW"
BASE COUNT          550 a          604 c          570 g          585 t
ORIGIN      
        1 gcattgattg cgccttccat acctttaaca atcaggtcag ccgcttcagt ccaacccata
       61 tggcgcagca tcatctcagc ggagagaata atagagcctg ggtttacttt gtcctgtccg
      121 gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt
      181 gcgccagggg cgataccgat accaccaacc tgcgctgcca gggcgtcaga aatgtagtca
      241 ccgttcaggt tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc
      301 aggaatgcat cagcaatcac gtctttaatg acgatctctt tgccggtatt cgggttttta
      361 actttcagcc acgggccgcc gtcgatcagt tcaccgccaa attcttcacg cgccagttgg
      421 tagccccagt ctttaaacgc gccttcggtg aacttcatga tgttgccttt gtgcaccagg
      481 gtcacagagt cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg
      541 gtgccttctt ccgaacacgg cttaataccg ataccgcaat gttccgggaa gcgaattttc
      601 ttcacgccca tctcttcacg caggaattta atcactttct cagcgtcggc agagtctgct
      661 ttccattcga tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc
      721 agttcagggt gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg acgcaggcag
      781 atgtagagat ccagttcctg gcgcagggca acgttcagag agcgaatacc gccaccaacc
      841 ggagtggtca gcgggccttt aatggcaacg cgatattcac gaatcagatc aagggtttca
      901 gcaggcaacc agacgtcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc
      961 atccaggaga ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc
     1021 agcatggctg gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga
     1081 ttttcaggaa cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca
     1141 actactttac tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca
     1201 cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga
     1261 tttgtaatta gcttgtcaat actaccctat tgtttgccgt catgaaagat gcgcgttatt
     1321 aggctataat gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa
     1381 atcaccaggt taagcgattc agctcgcaac gttctaccag gcgtaaacct gacaaccagc
     1441 ccacgcgtgt gatcctgttc aataaaccct acgatgttct tccgcagttc accgatgaag
     1501 ccggacgcaa aacattaaaa gaattcatcc cggttcaggg ggtgtatgcg gcaggtcgtc
     1561 tcgaccgcga cagcgaaggg ttactggtgc tgaccaataa cggcgcgttg caggcgcgtt
     1621 taacccagcc gggtaaacgc accggaaaaa tctattatgt gcaggtggaa ggtattccca
     1681 cacaagacgc acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc
     1741 ccgcaggcgc ggagctggtt gaagaacccg cgtggttatg gccgcggaat ccaccaattc
     1801 gtgagcgcaa aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc
     1861 gccaggtgcg ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgctatg
     1921 cgatggggga ttactctttg gataatcttg ccaatggcga atggcgagac gcgacagatt
     1981 aaggatgcaa aatgtttaaa ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca
     2041 aatttttagt cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc
     2101 acctggaagc cgatgaaacc ttagtggaag ccgccgcccg tgagctgtgg gaagaaaccg
     2161 gcattagcgc gcagccgcaa cactttattc gtatgcatca gtggattgcg cccgataaaa
     2221 cgccgttttt gcgtttcctc tttgccattg agcttgagca aatatgcccg acgcagcctc
     2281 atgacagcga tatcgactgc tgccgttgg
//
LOCUS       DAOFSV010000296         2299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.297, whole genome shotgun sequence.
ACCESSION   DAOFSV010000296 DAOFSV010000000
VERSION     DAOFSV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.297"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(30..746)
                     /gene="arcA"
                     /locus_tag="QBH27_004792"
     CDS             complement(30..746)
                     /gene="arcA"
                     /locus_tag="QBH27_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDQ7016990.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            842..982
                     /locus_tag="QBH27_004793"
     CDS             842..982
                     /locus_tag="QBH27_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016991.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            1382..2068
                     /locus_tag="QBH27_004794"
     CDS             1382..2068
                     /locus_tag="QBH27_004794"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDQ7016992.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAYLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT          639 a          513 c          528 g          619 t
ORIGIN      
        1 gccgtttttt ttgacggtgg taaagccgat taatcttcca gatcaccgca gaagcgataa
       61 ccttcaccgt gaatggtggc gatgatttcc ggcgtatccg gcgtagattc gaaatgttta
      121 cgaatacggc ggatcgtcac gtctacagta cggtcgtgcg gtttcagctc acggccggtc
      181 attttcttca gcagttcagc acgggactga attttacctg ggttttcaca gaagtgaagc
      241 atggcgcgga actcgctgcg cggcagcttg tactgctcgc catcagggcc gatcaacgaa
      301 cggctgttga tgtccagttc ccaaccattg aacttgtagc tttcaacgct acgacgttct
      361 tcgctgacag tacccagatt catggtacgg gacagcaggt tgcgtgcacg aatagtcagt
      421 tcacgcgggt tgaacggttt ggtgatgtag tcatctgcac cgatttcgag gccgagaatt
      481 ttatcgactt cgttgtcacg gccagtcagg aacatcaacg caacattcgc ctgctcgcgc
      541 agttcacgcg ctaacagaag accgttctta cccggcagat tgatatccat gatcaccagg
      601 ttgatgtcat attcagagag gatctgatgc atttccgcgc catctgtcgc ttcgaaaaca
      661 tcatagcctt ccgcttcgaa aatacttttc aacgtgttgc gtgttaccaa ctcgtcttca
      721 acgataagaa tgtgcggggt ctgcatgttt gctacctaaa ttgccaacta aatcgaaaca
      781 ggaagtacaa aagtccctga cctgcctgat gcatgctgca aattaacatg atcggcgtaa
      841 catgactaaa gtacgtaatt gcgttcttga tgcactttcc atcaacgtca acaacatcat
      901 tagcttggtc gtgggtactt tccctcagga cccgacagtg tcaaaaacgg ctgtcatcct
      961 aaccatttta acagcaatat aacaggctaa gaggggcagg acacccaata aaactacgct
     1021 tcgttgacat atatcaagtt caattgtagc acgttaacag tttgatgaaa tgatcgtatc
     1081 taaatgctag ctttcgtcac attattttaa taatccaact agttgcatca tacaactaat
     1141 aaacgtggtg aatccaatta tcgagattta ttttttataa aattatccta agtaaacaga
     1201 aggatatgta gcatttttta acaattcaat cgttagtaca gtcaggaaat agtttagcct
     1261 tttttaagct aagtaaaggg ctttttctgc gacttacgtt aagaatttgt aaattctcac
     1321 cgcgtaataa gttgacggtg atcaccctgt tcgcggttat ttggtcaaga agagtggcaa
     1381 tatgcgtata acgattattc tggttgcacc cgccagagca gaaaatattg gggcagcggc
     1441 gcgggcaatg aaaacgatgg gttttagcga actgcggatt gtcgatagtc aggcgtacct
     1501 ggagccagcc gcccgttggg tcgcacatgg atctggtgat attattgata atattaaagt
     1561 tttcaagaca ctagctgaat cgttacacga tgtcgatttc actgtcgcca ccactgcgcg
     1621 cagtcgggcg aaatatcatt actacgctac gccagtagaa ctggtgccgc tgttagagga
     1681 aaaatcttca tggatgagcc atgccgctct ggtgtttggt cgcgaagatt ccgggctgac
     1741 taacgaagag ttagcgttgg ctgacgttct tactggtgtg ccgatggtgg cggattatcc
     1801 ttcgctcaat ctggggcagg cggtgatggt ctattgctat caattagcaa cattaataca
     1861 acaaccggcg aaaagtgaca caacggcaga ccaacatcaa ctgcaagctt tacgcgaacg
     1921 agccatgaca ttgctgacga ctctggcagt ggcagatgac ataaaactgg tcgactggtt
     1981 acaacaacgc ctggggcttt tagagcaacg agacacggca atgttgcacc gtttgctgca
     2041 tgatattgaa aaaaatatca ccaaataaaa aacgccttag taagtgattt tcagcttttc
     2101 attctgactg caacgggcaa tatgtctctg tgtggattaa aaaaagagtg tctgatagca
     2161 gcttctgaac tggttacctg ccgtgatcaa attaaaattt tattgactta ggtcactaaa
     2221 tactttaacc aatataggca tagcgcacag acagataaaa attacagagt acacaacatc
     2281 catgaaacgc attagcacc
//
LOCUS       DAOFSV010000297         2293 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.298, whole genome shotgun sequence.
ACCESSION   DAOFSV010000297 DAOFSV010000000
VERSION     DAOFSV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.298"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..243)
                     /locus_tag="QBH27_004795"
     CDS             complement(<1..243)
                     /locus_tag="QBH27_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016993.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNE"
     gene            466..663
                     /locus_tag="QBH27_004796"
     CDS             466..663
                     /locus_tag="QBH27_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDQ7016994.1"
                     /translation="MNTNRILRKKEVLHLTGNSSATLYRLISKGFFPLSKRLTGDNGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            665..1132
                     /locus_tag="QBH27_004797"
     CDS             665..1132
                     /locus_tag="QBH27_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016995.1"
                     /translation="MKEIKSKTSSAKKTLVPWFETMGKQVGLSQETISAVLKEQRLRI
                     RKLNYASAYTVSNILIQDCGGADDMWSNYAKLALILERCKYIPCGSRACAYCSTAQQL
                     HYQGVDVTGEYALYQLPELFLDFLETNDKNSSYHWDYNKLVERHEDAQARSFG"
     gene            1220..2074
                     /locus_tag="QBH27_004798"
     CDS             1220..2074
                     /locus_tag="QBH27_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7016996.1"
                     /translation="MNDSYYFRTSDPDYIRHAPSMLEILSKAFPTIITQQESEYELQR
                     LNHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNIGALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEERIFWVPQLCLFLPNDSTLIKGLKAHMSRSGGAFIDASTLNTPVIVLRYKD
                     PARLISCALNPVWHTADYTLTDKDALVKSRMELLKGRTLLKSLLTLDSLGTGVVSFSF
                     GQPPGKVH"
BASE COUNT          623 a          526 c          546 g          598 t
ORIGIN      
        1 ctcattgagc aggtcattaa acatgtggtt cacgcaggca ctcagcacat ctgccgcttt
       61 ctccgcagcg aatttatccg ggctaaaacc cgctcccgtc agtttccggg caatcagtgc
      121 cagtttttgc cgggtatccc gtcccagata aagcttcttc tccagaaatt caaagacctc
      181 gttactgctt ttccgttccc ggtggcatct ggcgtcagcc ttgttgcttt ggttaccaat
      241 catagtcacc tccatgacag tgttttgtac aaaatatcac cattgagcct cagtgaaaat
      301 tgcttcttta tatgaccgtt ctggagacag aaatagatga tctcgaatga catccacatt
      361 aaagatgccc ttaacgtgct caacgaaggc atcgttgagt gaatccttgc gttttttggt
      421 ggttgtaaat aacccgctcc tgaaacagct caaaggagca caagcatgaa caccaaccgt
      481 attttgcgta aaaaagaggt acttcatctg acggggaact cgtcagcgac gttgtaccga
      541 ctcatctcga aaggattctt tcctttgtca aagaggctaa cgggcgacaa tggccgggcg
      601 gtaggctggc tggagagtga tatcaataac tgggttaaca gccgtatgca ggcgggagaa
      661 tgaaatgaaa gagataaaat cgaaaaccag ttctgcgaaa aaaacgctgg ttccttggtt
      721 cgaaactatg gggaaacagg ttggtttatc acaagaaacg atatcagcag tgttaaagga
      781 gcagcgttta cggatacgta agcttaacta cgcatctgca tatacagtgt ccaatattct
      841 catacaggat tgcggcgggg ccgatgacat gtggagcaac tacgctaaac tggcgcttat
      901 ccttgagcgt tgtaaataca ttccctgcgg gagcagggcc tgtgcatatt gttctaccgc
      961 tcagcaatta cattatcagg gggtggatgt aaccggggaa tacgctttat atcagttgcc
     1021 tgagttattt ttagattttt tagaaacaaa cgacaaaaat tcctcttatc attgggatta
     1081 caataagtta gttgagcggc atgaagatgc tcaggcgagg tcattcgggt aatagccatt
     1141 tctgtatggt gaaaaactga catattaccc tgccactgca ttttggtggc agggtaatca
     1201 atacagctag gaatattaaa tgaacgattc ttattacttc agaacatctg acccggatta
     1261 cattcgtcac gcaccgtcaa tgcttgagat tttaagtaaa gcattcccca ctattattac
     1321 ccagcaggaa agtgagtatg aactgcagcg tcttaaccat ttactaaata aactgaaagg
     1381 cagcaatcgc tgctacaaca tcattgctca aaaactaagg cagtgcagaa acggatctcc
     1441 atgcaacagt cttatatgcc cgcattgcca gcgggaacgc attctggcgc aactggctat
     1501 gttgcatgtc ttgccgggga attcagcaga atacgtggga gtggtcctgt ttttcaacaa
     1561 ggatacgcaa actccgccgc catggaagaa cattggggcg ctcagggcgc aaatcggtcg
     1621 ttataagcag aggataagcc gggtacttaa ccgcctgggg tacgccggtc cggccacagg
     1681 caccttcagt atgatgcgac atatgcctga tggcccagaa gagcgtattt tctgggttcc
     1741 acagttgtgt ttgttcctgc ccaatgacag tacgttgatt aaggggctaa aagcgcatat
     1801 gtcgcgaagt ggtggggcat tcattgatgc atcgacgctt aacacaccag tgatcgtgct
     1861 caggtataaa gatcccgcga gactgatttc atgcgccctg aatccagtct ggcacacagc
     1921 agattacaca ctgactgata aagacgctct tgttaagtca agaatggagc tactgaaagg
     1981 ccggacactg ttaaaaagtc tgcttaccct ggactcactc ggaacaggtg tcgtttcttt
     2041 ttcgtttggg caacccccag ggaaagtgca ctgatttacc ggtaatgcag caactgcaat
     2101 gcacttttgc tgttgattaa attgcagtgg aacgacgaat aacagcggca accactgcat
     2161 tttcgttgtt gtaaaactgc aaagcttgcc agtgattttg atggcgctga tgcactttaa
     2221 cattccaact gtctcgattt taacgcaacc gtctgttttc tgcaggggct cacggatgcc
     2281 tctgcacacc cga
//
LOCUS       DAOFSV010000298         2282 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.299, whole genome shotgun sequence.
ACCESSION   DAOFSV010000298 DAOFSV010000000
VERSION     DAOFSV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.299"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..944)
                     /gene="cbl"
                     /locus_tag="QBH27_004799"
     CDS             complement(<1..944)
                     /gene="cbl"
                     /locus_tag="QBH27_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDQ7016997.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDY"
     gene            complement(1046..1963)
                     /gene="nac"
                     /locus_tag="QBH27_004800"
     CDS             complement(1046..1963)
                     /gene="nac"
                     /locus_tag="QBH27_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDQ7016998.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT          612 a          587 c          499 g          584 t
ORIGIN      
        1 tgataatcaa tttcctcttc actgttttcc atcacctggc gcttgatatc ctcaactgac
       61 agtcctgcat tacaaagttc cagaaagcgc cagacatagt tacgctgaag ttgccctcgc
      121 ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag gcggattaaa
      181 ttcttttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc aagcccaaga
      241 gcaacatagg ttttaatgac atcagaatcc tgtgcactta atacaatatc tgccggcaaa
      301 cctttgcggg caaatgcgtc atcaatgcgt gagcgccccg taatcccctg tcggtaagta
      361 attaacggcc acttcgctat tgattccagc gtcaacggtg taatttgcgt caagggatga
      421 tcgagtggaa caagcaaact atggtgccaa cgaaaccacg ggaaggcgac gagctgcggg
      481 tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg caacaacgtc
      541 gcaatttcct gtggtgtacc ctggattagc tccagccgaa cctccgggaa aagttcgcga
      601 aaagctttaa tgacctcagg caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg
      661 agaacgccag acgtatcgtt ggtaaacagg tctgcgagcc gacgaacatt actggcttca
      721 ttcagaatgc gttctgcaat gaccagtaat gctttgcccg gttcagtcat gcccagcagt
      781 cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat cctccagttc ccgaatatga
      841 cggctaacgc ctgactgtga ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc
      901 tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa ctccgggcag
      961 ttcagatatc ccgttattgt taaagtctaa tgcccggcat aacaaataat aaaaacccgc
     1021 atcttattcc atcccgatat aacgcttagc tcaccaattg ccactgcctt ttttccatca
     1081 ctggagaact aatcactgac attaacaact ctttcactgc ctgtgcctgt ggtgacaagt
     1141 ttgctctggc gggtaaattt aaggacaaag agagactcat tgaaggagtg gtaatgcgtg
     1201 acatccaccc atttactgcg ccacataacg aacgcgcagc cgattcaggt aatactgcaa
     1261 cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat agactcaatt tcaccaatga
     1321 cttttgccgt gagccgccgt agggaaaaag cctcatcaac acgaagtcta acagcgctgt
     1381 aatcactggg gagaaaaagg ttcatttgcg caatagcatt aacatcaacg ctttgccccg
     1441 ggcaatcttg agttcctacc aggaaaagat cttctttcag caaagcctga ctggataccc
     1501 cagccacagg ggaatgctca taaatcaccg ccatatcgag ttggtgattt atcagttttt
     1561 cgttaagcac tgcaccacta ttttcatgaa gatagataac gacctccggg aattcagcgc
     1621 gaaccgcctg taataagggc atggtgatgg atgacgcagc ggttcctggc gcaaagccaa
     1681 tcgagacttg ccccgataat gactgaccaa cgttatgcac cgccagttgg gcctgctcac
     1741 actgacgtaa aatggcccgt gcatgggtat agagaatttt tccggcgcct gttggcgtaa
     1801 cgccccgttt tgtgcgaatc aaaagctgtt gatttaactc accttccagt gtggcaacct
     1861 gctggctgag cgctggttgt gcgatatgca atacttcagc agcctgggtc aggctaccaa
     1921 tatctacaat ttttacgaag tatttcaggc gtctgaagtt catgttgcct ccggttttta
     1981 agaatcggcc caagtgccgc cattacttac aaccagaatg caagatgctt gccagttcta
     2041 ttttagcgtt gatgcgcaag ctaaccaact gtcaaataag agattatgat agattcgtca
     2101 ttggcccttt ttatcagcag tcgcattcac ctgccctata aaaggggggt atgcaccacg
     2161 atggttcatt acccaataag attgaaagct caccactttg ttgaaattgt cagcaaacaa
     2221 acaaaaaaat gcatttcacc ctttgacatc accatgcact gccattaata tgcgccccgt
     2281 tc
//
LOCUS       DAOFSV010000299         2241 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.300, whole genome shotgun sequence.
ACCESSION   DAOFSV010000299 DAOFSV010000000
VERSION     DAOFSV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.300"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(316..1080)
                     /locus_tag="QBH27_004801"
     CDS             complement(316..1080)
                     /locus_tag="QBH27_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDQ7016999.1"
                     /translation="MNNQTIRTSVPALDKTVRVCNYLFSSPGATFSQIQQDLDLPKSS
                     TSSLLNALVEHHLLRQERGRFFLGLKLYEWGNRSLEQFDISKVARPVLEKLRDSTDLI
                     CHLGILDEVAPIYILKLESTQPIGIRTWVGKKLPLHSSGVGKALCAWLPQDVIDTLLP
                     EETLPRFTQTTITRKSDLIQEFARIRERGWAFDNAEDSPGIYCIAAPVFNQTREVIAA
                     ISISGVEIQIPKEKIEYYSTLVLDACQELSEKLKYI"
     gene            1320..2219
                     /locus_tag="QBH27_004802"
     CDS             1320..2219
                     /locus_tag="QBH27_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDQ7017000.1"
                     /translation="MLTAFRGIFPPVPTIVDAQGKLDKAGMARMIDHVLENGANGMLI
                     LGSGGEFSQFSSQQRKEIAAFCLSHVDGKVPVLVGIACAGTAETIDLGHHAQEHGADG
                     VMVVNPYYAKLSDDARFMHYKSIADSLKIPVFLYNFPDLTGQDIGLEVITRLAREVPN
                     IVGIKDTIDNISHTREVINRVHAFRPDFIIFSGYDEYMLDTLLLGGHGGIPATFNFAP
                     NITRGIYQAVMENDLDKARYFQQQLAKLSPLYTLEQPFFGVIKTAIKLSGLDISTAVM
                     APALPLSDDKVARVKAILEHIHQ"
BASE COUNT          660 a          467 c          458 g          656 t
ORIGIN      
        1 ttgtgtataa gagacagcct gatagggggg cgcatacatt atgcatacat actctcggat
       61 attgtaaaag ttttttatat aattactaag ttagctacca tcaataaaat attgacgtta
      121 gcaggtaaca tcaacttaat atataaaaat attcttaggt gaaagtaact gttcaggata
      181 aaataaaatc atcgcttaaa aagaaaaaca ctctctttgt tatatacgta ctctgataaa
      241 tgccagataa aactattaat tttcataaaa atataatacc atatattatg atgctataca
      301 tcttacatat tcaattcaga tatattttaa tttttccgat agttcttgac acgcatcaag
      361 aaccaaagtt gaataatatt caattttttc tttaggtatc tgaatttcca cgccactaat
      421 gctgatagct gcaattactt cacgagtctg attaaatacc ggagcagcaa tacaataaat
      481 tcccggtgaa tcctctgcgt tatcaaaagc ccatccacgt tcacgaatac gtgcaaattc
      541 ctgtattaga tcagatttac gagtgatagt tgtttgcgta aatcgtggca acgtttcctc
      601 aggcaacaat gtgtcaatga cgtcttgagg taaccacgca cataaagcct taccaactcc
      661 agaactatgg agtggcaact tcttgcctac ccacgttctg atgccaattg gttgtgtact
      721 ctcgagtttc aaaatataga ttggagcgac ttcatcgaga atgcccagat ggcagataag
      781 atcagtactg tctctcagtt tttctagaac cggcctcgct accttactga tgtcaaactg
      841 ctccagagat cgattccccc attcatacaa tttcagccca agaaaaaaac gccctctttc
      901 ctgacgtaac agatgatgtt cgacaagggc attcagtaaa gatgatgtgc tgctttttgg
      961 cagatccaga tcctgctgga tctggctgaa ggtggcgcct ggagagctaa aaaggtaatt
     1021 gcagactctg acagttttat cgagtgctgg taccgatgtt ctgatggttt gattattcat
     1081 aaaaaaatat gattccttac aatactgaat agtatacccc attccccctt gatgcgcagc
     1141 ggcgcttagc acacatatta tacatggcct ccgcgtaccg gcatgcctct ataaacctga
     1201 cttatttcac acttttcata tcgcattgta aaaacatttg acatgcaaaa cctgaatttg
     1261 attcacttta ttcagcataa agaacttgtg ttccatatac agaacttagt gagagtgata
     1321 tgttgacagc gtttagaggt attttcccgc cagttcctac aattgttgac gcacaaggga
     1381 aactagataa ggctggcatg gccaggatga tcgaccatgt tttagaaaat ggtgcaaacg
     1441 ggatgttgat ccttggcagt gggggagaat tctcacaatt ttcttcccag caacgaaaag
     1501 agatagctgc attttgctta agtcatgtag atggcaaagt gcctgttctg gtggggatcg
     1561 cctgcgcggg aactgcagaa actattgatt taggacatca tgcacaggag cacggagctg
     1621 atggtgtgat ggtggtgaat ccgtattatg caaaactcag cgatgatgcc cggtttatgc
     1681 attacaaatc tattgccgat tcacttaaaa ttccagtgtt tctctataac tttcctgatc
     1741 tgaccggtca ggatattggg cttgaggtca ttacccggct ggcccgggaa gttccaaata
     1801 tcgtgggtat caaggatacc atcgataata tcagccatac ccgggaagtc atcaaccgtg
     1861 tacatgcttt tcgtccggat ttcattatat tcagcggata tgacgaatat atgctggata
     1921 ccctgttact gggcggccat ggtggtattc cggcgacatt caattttgct ccaaatatca
     1981 cacgtggtat ttatcaggcc gttatggaaa atgatcttga taaagcgcgt tattttcagc
     2041 aacaactggc aaaactgtct ccgttataca cactggagca gcctttcttt ggcgtgataa
     2101 aaactgccat caaactgagt ggactggaca tatctactgc agttatggca ccggctcttc
     2161 cgctcagtga tgacaaggtc gccagggtga aagccattct ggaacacatt caccagtgat
     2221 tacgaggatc gtaagatgaa t
//
LOCUS       DAOFSV010000300         2238 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.301, whole genome shotgun sequence.
ACCESSION   DAOFSV010000300 DAOFSV010000000
VERSION     DAOFSV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.301"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            277..1689
                     /gene="pykF"
                     /locus_tag="QBH27_004803"
     CDS             277..1689
                     /gene="pykF"
                     /locus_tag="QBH27_004803"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDQ7017001.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2000..2236
                     /gene="lpp"
                     /locus_tag="QBH27_004804"
     CDS             2000..2236
                     /gene="lpp"
                     /locus_tag="QBH27_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDQ7017002.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT          625 a          544 c          511 g          558 t
ORIGIN      
        1 ctgtttttgt tttccttttg gattaatttc agcgtataat gcgcgccaat tgactcttga
       61 atggtttcag cactttggac tgtagaactc aacgactcaa aaacaggcac tcacgttggg
      121 ctgagacaca agcacacatt cctctgcacg ctttttcgat gtcacctatc cttagagcga
      181 ggcacaacca ctttcgtaat accggattcg ctttccggca gtgcgcccag aaagcaagtt
      241 tctcccatcc ttctcaactt aaagactaag actgtcatga aaaagaccaa aattgtttgc
      301 accatcggac cgaaaaccga atctgaagag atgttagcta aaatgctgga cgctggcatg
      361 aacgttatgc gtctgaactt ctctcatggt gactatgcag aacacggtca gcgcattcag
      421 aatctgcgca acgtgatgag caaaactggt aaaaccgccg ctatcctgct tgataccaaa
      481 ggtccggaaa tccgcaccat gaaactggaa ggcggtaacg acgtttctct gaaagcgggt
      541 cagaccttta ctttcactac tgataaatct gttatcggca acagcgaaat ggttgcggta
      601 acgtatgaag gtttcactac tgacctgtct gttggcaaca ccgtactggt tgacgacggt
      661 ctgatcggta tggaagttac cgccattgaa ggtaacaaag ttatctgtaa agtgctgaac
      721 aacggcgacc tgggcgaaaa caaaggtgtg aacctgcctg gcgtttccat tgccctgcca
      781 gcactggctg aaaaagacaa acaggacctg atctttggtt gcgaacaagg cgtagacttt
      841 gttgctgctt cctttattcg taagcgttct gacgttatcg aaatccgtga gcacctgaaa
      901 gcgcacggcg gcgaaaacat ccacatcatc tccaaaatcg aaaaccagga aggcctcaac
      961 aacttcgacg aaatcctcga agcctctgac ggcatcatgg ttgcgcgtgg cgacctgggt
     1021 gtagaaatcc cggtagaaga agttatcttc gcccagaaga tgatgatcga aaaatgtatc
     1081 cgtgcacgta aagtcgttat cactgcgacc cagatgctgg attccatgat caaaaaccca
     1141 cgcccgactc gcgcagaagc cggtgacgtt gcaaacgcca tcctcgacgg tactgacgca
     1201 gtgatgctgt ctggtgaatc cgcaaaaggt aaatacccgc tggaagcggt ttctatcatg
     1261 gcgaccatct gcgaacgtac cgaccgcgtg atgaacagcc gtctcgagtt caacaatgac
     1321 aaccgtaaac tgcgtataac cgaagcggta tgccgtggtg ccgttgaaac tgctgaaaaa
     1381 ctggatgctc cgctgatcgt ggttgctact cagggcggta aatctgctcg cgcagtacgt
     1441 aaatacttcc cggatgccac catcctggca ctgaccacca acgaaaaaac ggctcaccag
     1501 ttagtactga gcaaaggcgt tgtgccgcag ctggttaaag agatcacttc tactgatgac
     1561 ttctaccgtc tgggtaaaga actggctctg cagagcggtc tggcacacaa aggtgatgtc
     1621 gtagttatgg tttctggtgc actggtaccg agcggcacta ctaacaccgc atctgttcac
     1681 gtcctgtaat attgcttttg tgaattaatt tgtatatcga agcgtcctga tgggcgcttt
     1741 ttttatttaa tcgataacca gaagcaataa aaaatcaaat cggatttcac tatataatct
     1801 cactttatct aagatgaatc cgatggaagc atcctgtttt ctctcaattt ttttatctaa
     1861 aacccagcgt tcgatgcttc tttgagcgaa cgatcaaaaa taagtgcctt cccatcaaaa
     1921 aaatattctc aacataaaaa actttgtgta atacttgtaa cgctacatgg agattaactc
     1981 aatctagagg gtattaataa tgaaagctac taaactggta ctgggcgcgg taatcctggg
     2041 ttctactctg ctggcaggtt gctccagcaa cgctaaaatc gatcagctgt cttctgacgt
     2101 tcagactctg aacgctaaag ttgaccagct gagcaacgac gtgaacgcaa tgcgttccga
     2161 cgttcaggct gctaaagatg acgcagctcg tgctaaccag cgtctggaca acatggctac
     2221 taaataccgc aagtaata
//
LOCUS       DAOFSV010000301         2223 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.302, whole genome shotgun sequence.
ACCESSION   DAOFSV010000301 DAOFSV010000000
VERSION     DAOFSV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.302"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(6..146)
                     /locus_tag="QBH27_004805"
                     /pseudo
     CDS             complement(6..146)
                     /locus_tag="QBH27_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            331..606
                     /locus_tag="QBH27_004806"
     CDS             331..606
                     /locus_tag="QBH27_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDQ7017003.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            603..1160
                     /locus_tag="QBH27_004807"
     CDS             603..1160
                     /locus_tag="QBH27_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDQ7017004.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1287..1457)
                     /locus_tag="QBH27_004808"
     CDS             complement(1287..1457)
                     /locus_tag="QBH27_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017005.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(1572..1841)
                     /locus_tag="QBH27_004809"
     CDS             complement(1572..1841)
                     /locus_tag="QBH27_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ7017006.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            1898..>2223
                     /locus_tag="QBH27_004810"
     CDS             1898..>2223
                     /locus_tag="QBH27_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDQ7017007.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVF"
BASE COUNT          618 a          521 c          467 g          617 t
ORIGIN      
        1 aaaaattaga agcggtaacc tgcagagaac ataaataccc acgggtcaag gcgtacgttg
       61 tcagaataac gccctgcagg cagcactgcg ccgttggatt tatatttcac ttcggtatcg
      121 atatccatgt accagaccga catgttttaa aaaatgcaat aagacattgt atctgaaaga
      181 aaaaacagga tttgaggcgc ttttttatac cgtttttccg tgtttttaac tgcctgattt
      241 tattgaaatc ttttttagtt ttgcagaaag gattttttgc tcgatcgctt taattttata
      301 cattcctgat aacggaaatt tttttgcccg ttgacagttt ttgttctccg gggctatatt
      361 cctcacacaa ccgcaaaatc ggttgtcggg attggcgtct cggataacat cacaacgcat
      421 agccgcgttt gcggtttttt ttgcgttaag cattgccaca ttcgcattat ggtggggcgt
      481 gcaggggcgg agaaatccgc gccgggtttt gtgatgaccg gttacgccaa ccctgtacgt
      541 ctcaccacct ccctgattgg cgtcagaggt ggtgatcatc caattaattg gagtcatcac
      601 atatgaatac agctataact ttttctaacg agtcaccaaa agtctttttc aaagatggtc
      661 gcctggttac cacctctcaa gctgttgccg actacttcga taaacaacat aaacacgttt
      721 tagctaagat tgacacactc gactgctctc ccgaatttac atcagccaac ttttcggccg
      781 atgttcagac tgtcgaaatt ggcaacggcg cagaacgtga atcacgatgc tacctgatca
      841 caaaagacgg cttcatgttc ctcgtaatgg gatttactgg caaaaaagca gccagactaa
      901 aagaagctta cattgaaaaa tttaacgcaa tggaagaaga actccacaaa cgtcctcctg
      961 cagcacaaaa ctcccccgcc ccaaataatg gatgcgcatt actgatccac ttcgataaac
     1021 acggtcaggt cgagttcaca gaaaaagtcc ccgccgatgc gatggtatgc acgctggaac
     1081 ggtttaaatt ttatctggag caacatgggt ggatcgttgc ccgtaaagaa caattggtgg
     1141 aaaggttgat gcggttttaa tgaatgcaaa accccggatg atcaccgggg ttaattttat
     1201 cgagcgtgat tgccgctaat cgccatcgct ctgattgagc gtaaatcatt caggttattc
     1261 agttcttcct tctgctctcg ctgacgtcaa taaatcccat cattgcgatc aacctcatcc
     1321 ttaaccattg atgcgagcag ttcttccagt ttctgcgctg acagctttac ctggtgatct
     1381 tcagcatctt cccgggctat tgtggttcgc gcagtggctg attttgctga catcaccaca
     1441 gggggcagac ggaccattga accatggcct ccggaacaaa taagggcaaa aataacaacc
     1501 actatcgaaa gctcacaaac taaccgcagc acgttcctgc atacgacgtg tctgcggcat
     1561 aatcccaatg attactccct gacaggattt gcaggccact caatatcagg tgcagttgat
     1621 gtatcaacac gattcaacaa tacccgatat ttattccatg cctccagcaa cgatctttct
     1681 tcctccgttg cgatttccag atctacagca tcctgcagtg gcgcaatata ctcactgaat
     1741 tcctggatgt agaactgtgt ggtgacggtc ttccagccat tcggctcctg ctgtatcgaa
     1801 gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct tgtaattcat
     1861 cgccataatt gatttaattc acaaataaaa ctataacatg gtgaaatcaa tgaaaaaaaa
     1921 cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg actggtgggt
     1981 tttgggagta tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc ttcttccaca
     2041 ctttctggaa catgtaggta acaaccatct ggatattggt gttggaactg ggttttacct
     2101 tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag ctagcctgaa
     2161 cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattagcc atgatgtttt
     2221 tga
//
LOCUS       DAOFSV010000302         2206 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.303, whole genome shotgun sequence.
ACCESSION   DAOFSV010000302 DAOFSV010000000
VERSION     DAOFSV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.303"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..409
                     /locus_tag="QBH27_004811"
     CDS             <1..409
                     /locus_tag="QBH27_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HDQ7017008.1"
                     /translation="VQEQLEHYFIENQEIKKKLPPVLYGAAEQIKQELGYRLGYIIVS
                     YSKSLKGIITMPFALIRECVFEKKRKKSYGVDVPLYLYADADKAERVKKHLSYQLGQA
                     IISSANSIFGFITLPFKLIVLVYKYRRAKIKGC"
     gene            complement(506..1540)
                     /locus_tag="QBH27_004812"
                     /pseudo
     CDS             complement(506..1540)
                     /locus_tag="QBH27_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178350.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            1701..>2206
                     /locus_tag="QBH27_004813"
     CDS             1701..>2206
                     /locus_tag="QBH27_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ7017009.1"
                     /translation="MNAEYINLVERKKKLGTNIGALDFLLSIHKEKVDLQHKNSPLKG
                     NDNLIHKRINEYDNVLELSKNVSAQNSGNEFSYLLGYADSLRKVGMLDTYIKIVCYLT
                     IQSRYFKNGERVKLFEHISNALRYSRSDFLINLIFERYIEYINHLKLSPKQKDFYFCT
                     KFSKFHDYT"
BASE COUNT          636 a          374 c          434 g          762 t
ORIGIN      
        1 agtgcaagag cagttggaac actattttat agaaaatcag gaaattaaaa agaaacttcc
       61 acctgtgcta tatggagcag ctgagcagat aaaacaagag ttaggttatc gacttggtta
      121 tattatagtc tcgtattcta aatccctcaa ggggattatt accatgccat ttgcacttat
      181 ccgtgagtgt gtttttgaaa aaaaacgtaa gaagagttat ggcgttgatg tgccactcta
      241 tttatatgct gatgctgata aggctgaaag agttaagaaa catttatctt atcaattagg
      301 gcaggctatt atctccagtg ctaattcgat atttggattc attacccttc catttaagtt
      361 aattgttctt gtttataaat ataggagagc taaaatcaag ggctgttaaa aatgtgaacc
      421 ctaatgagat atattgcaaa ttttattttt ctctttgtgg tgttttgctt tcgttaaaat
      481 agttagttat tttatttata taatatcacg cattataata ccaatttata cttttgcaag
      541 tgaccgtata gattcgccac atattgcaaa ttttgttctc tcgtaaaata ttttcttctg
      601 gtgtcagtaa ttcgagcact tcatgctgtc ttttactgca gtatagtact aggttttcag
      661 ctagtttctg attaaatatg tttagctctt taaagagtct atttatttaa attcaatgaa
      721 ctgttctttc ggcgttcgct taaaacgttc agaggtgcgt tctaggcgta aaggggtagg
      781 tccatagggc ttgctagcgg attcctgcgg tgctttgtcg aagtttcctg ggaactcttt
      841 cccgttatct gcgatgccct ggttgatatc gatagggaat agccggtctg cacggcaaaa
      901 gaagagtttg atgatatcac agttaagtga cggcacggcc tgtgccagag cgtagttgct
      961 gtgttcgtcg atcatggtaa tgacataaca gcgcagctcg cccattctga gctcaatagc
     1021 atctatacca acgagctcac tgctctttac cgggcgatag cgttttgatc tgcgggttgg
     1081 aggattgtat tttttatgaa cagtgttttt ccttggggag gcagtcacat cggtatcagt
     1141 cgcattttat cgtgtgcggc cgtgaacatc ctgccgatgg ttgaaatgct cggacagacc
     1201 aggcggtgct gttcactcca cggcttcaag caaacaaaaa tctgttcttc taggttggga
     1261 agctctattc tcagtcgacg tatttcttac agaattacga gatgccattg ctttgtgcga
     1321 tgtactagcg gagctttgct acgcgaaata ggtgcctctg ggccataata cagcgttcgt
     1381 gtggatacag caaaaacttc cgcaactgta ttgatctcat gtttctccca gaagtatatt
     1441 ttttcatcct taattttgta atctcaggta taacaaagtg tttcatcaca tagatgttgg
     1501 catggtaatg cctcaaatat ccgccgcaga tacgttgcat caacttagca tttccctcgc
     1561 ttgtccggag ataattgcaa tatctctgtg agcttacact gtgacattcg ttgagtttta
     1621 gtgatgtttt taaagattta tatttataat atttagtaaa tgcagtttta ttctcatttt
     1681 atttatcatt aagtgaatgt atgaacgcag aatatataaa tttagttgaa cgtaaaaaga
     1741 aattagggac aaatattggt gctcttgatt ttttattatc aattcataag gagaaagttg
     1801 atcttcaaca taaaaactcg cctttaaaag gtaacgataa ccttattcac aaaagaataa
     1861 acgaatacga caatgtactt gaactatcta agaatgtatc agctcagaat tctggcaatg
     1921 agttttctta tttattggga tatgcagatt ctcttagaaa agttggtatg ttggatactt
     1981 atattaaaat tgtttgttat ctaacaattc aatctcgtta ttttaaaaat ggcgaacgag
     2041 ttaagctttt tgaacatata agtaacgctc tacggtattc aaggagtgat tttctcatta
     2101 atcttatttt tgaacgatat atcgaatata taaaccatct aaaattgtcg cccaaacaaa
     2161 aagattttta tttttgtacg aagttttcaa aatttcatga ttatac
//
LOCUS       DAOFSV010000303         2199 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.304, whole genome shotgun sequence.
ACCESSION   DAOFSV010000303 DAOFSV010000000
VERSION     DAOFSV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.304"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..500
                     /locus_tag="QBH27_004814"
     CDS             <1..500
                     /locus_tag="QBH27_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDQ7017010.1"
                     /translation="RLQVQSNLPWWLVCRGAIHKFRCVPHLSGRRFEHGVTDCYTLFR
                     DAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSV
                     PNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWHASAFTGIYNDLAA
                     ASTFE"
     gene            398..1045
                     /locus_tag="QBH27_004815"
     CDS             398..1045
                     /locus_tag="QBH27_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDQ7017011.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1106..>2199
                     /locus_tag="QBH27_004816"
     CDS             1106..>2199
                     /locus_tag="QBH27_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDQ7017012.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPS"
BASE COUNT          462 a          559 c          727 g          451 t
ORIGIN      
        1 ggcggctgca ggtgcagagt aatttgccgt ggtggctggt ttgccggggg gcgattcaca
       61 agttccgctg tgtgccacat ctttccgggc ggcgctttga gcacggggtg acggactgtt
      121 acacgctgtt ccgggatgct taccatctgg cggggattga gatgccggat tttcatcgcg
      181 aggatgactg gtggcgtcac ggtcagaatc tctatctgga taatctggag gccacagggc
      241 tgtatcaggt gccgttgtca gcggcgcagc cgggcgatgt gctgctgtgc tgttttggtt
      301 catcggtgcc gaatcatgcc gccatttact gtggtgacag cgagctgctg caccatattc
      361 ctgaacaact gagcaaacga gagaggtaca ccgacaaatg gcagcgacgc acacactccc
      421 tctggcgtca ccgggcatgg cacgcatctg cctttacggg gatttacaac gatttggccg
      481 ccgcatcgac cttcgagtga aaacggggtc cgaagccatc cgggcgctgg ccatgcagat
      541 cccggcgttt cgtcagaaac tgagcgacgg ctggtaccag gtacgcattg ccgggcgtga
      601 tgcaggtgaa accgaattgt ctgcccgtct taatgagccg ctggcaaatg gtgccgtgat
      661 ccacatcgtg ccgcgtctgg cgggagctaa aagtggcggt gtgtttcagg tggtgctggg
      721 ggcggcgctg attgcggtgg catggtggaa ccctgtgggc tggctgggtg ccgcggctgt
      781 atcgggcatg tatgcggcag gggccagtat gatcctgggc ggagtggcgc agatgctggc
      841 accgaaagcc aggacgccca cggcagccag tacagataac ggcaaacaga acacctattt
      901 ctcctcactg gataacatgg ttgcccaggg caatgttctg cccgttctgt acggtgaaat
      961 gcgcgtgggg tcgcgggtgg tctctcagga gatcagcacg gcagacgaag gggatggtgg
     1021 tcaggttgtg gtgattggtc gctgatgaaa aacgttttat gtgaaaccgc ctccgggcgg
     1081 ttttgtcgtt tatggagcat gacgaatggg taaaggcagc agtaaggggc ataccccgcg
     1141 cgaagcgaag gacaacctga aatccacgca gttgctgagt gtgattgatg ccatcagtga
     1201 agggccggtt gaaggtccgg tggatggatt aaaaagcgtg ctgctgaaca gtacgccggt
     1261 gctggacact gaggggaata ccaacatctc cggtgtcacg gtggtgttcc gggcaggtga
     1321 gcaggagcag actccgccgg agggatttga atcctccggc tccgagacgg tgctgggtac
     1381 ggaagtgaaa tatgacacgc cgatcacccg gaccatcacg tcggcaaaca ttgaccgtct
     1441 gcgctttacc ttcggtgtgc aggcactggt ggaaaccacc tcaaaggggg acaggaatcc
     1501 gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt ggctgggtga cggaaaaaga
     1561 catcaccatt aagggtaaaa ccacttcaca gtatctggcc tcggtggtgg tggataacct
     1621 gccgccgcgc ccgtttaata tccggatgcg caggatgacg ccggacagca ccacagacca
     1681 gctgcagaac aaaacgctct ggtcgtcata caccgaaatt atcgatgtga aacagtgcta
     1741 cccgaacacg gcactggtcg gcgtgcaggt ggactcggag cagttcggca gccagcaggt
     1801 gagccgtaat tatcatctgc gcgggcgcat tctgcaggtg ccgtcgaact ataacccgca
     1861 gacgcggcaa tacagcggta tctgggacgg aacgtttaag ccagcataca gcaacaacat
     1921 ggcctggtgt ctgtgggata tgctgaccca tccgcgctac ggcatgggga aacgtcttgg
     1981 tgcggcggat gtggataaat gggcgctgta tgtcatcggc cagtactgcg accagtcagt
     2041 gccggacggc tttggcggca cggagccgcg catcacctgt aatgcctggc tgaccacaca
     2101 gcgtaaggcg tgggatgttc tcagcgattt ctgctcggcg atgcgctgta tgccggtatg
     2161 gaacgggcag acgctgacgt tcgtgcagga ccgaccgtc
//
LOCUS       DAOFSV010000304         2193 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.305, whole genome shotgun sequence.
ACCESSION   DAOFSV010000304 DAOFSV010000000
VERSION     DAOFSV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.305"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..500
                     /locus_tag="QBH27_004817"
     CDS             <1..500
                     /locus_tag="QBH27_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDQ7017013.1"
                     /translation="RLQVQSDLPWWLVCRGEIHKFRCVPHLTGRCFEHGVTDCYTLFR
                     DAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDVLLCCFGSSV
                     PNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLAA
                     ASTFV"
     gene            437..1039
                     /locus_tag="QBH27_004818"
     CDS             437..1039
                     /locus_tag="QBH27_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDQ7017014.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPRTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1100..>2193
                     /locus_tag="QBH27_004819"
     CDS             1100..>2193
                     /locus_tag="QBH27_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDQ7017015.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLLNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPS"
BASE COUNT          477 a          549 c          699 g          468 t
ORIGIN      
        1 ggcggctgca ggtgcagagc gatttgccgt ggtggctggt ctgccggggt gagattcata
       61 aattccgctg tgtgccgcat cttaccgggc ggtgctttga gcacggggtg acggactgtt
      121 acacgctgtt ccgggatgct tatcatctgg cggggattga gatgccggat tttcatcgcg
      181 gggatgactg gtggcgtcac ggtcagaatc tctatctgga taatctggag gccacagggc
      241 tgtatcaggt gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc tgttttggtt
      301 catcggtgcc gaatcatgcc gccatttact gtggtgatgg cgagctgctg caccatattc
      361 ctgaacaact gagcaaacga gagaggtata ccgacaaatg gcagcgacgc acacactccc
      421 tctggcgtca ccgggcatgg cgcgcatctg cctttacggg gatttacaac gatttggccg
      481 ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgggcgctgg ccacacagct
      541 cccggcgttt cgtcagaaac tgagcgacgg ctggtatcag gtacggattg ccgggcgtga
      601 tacaggtgaa acggaattat cagcccgtct taatgaaccg ctggcaaatg gtgctgtgat
      661 ccatatcgtg ccgcgtctgg caggagccaa aagtggcggt gtgtttcagg ctgtgctggg
      721 ggcggctgtt atggcggttg ctatatggat gccgggggta ggaattatgg cgagtaatct
      781 gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc tggcccctaa
      841 acccagaacc ccccgcacac agacaacgga taacggcaaa cagaacacct atttttcttc
      901 actggataat atggttgccc agggcaatgt tctgcccgtt ctgtacggtg aaatgcgcgt
      961 gggatcacgt gtggtatctc aggagatcag cacagcagat gaaggtgatg gtggtcaggt
     1021 tgtggtgatt ggtcgctgat gaaaaacgtt ttatgtgaaa ccgcctccgg gcggttttat
     1081 cgtttatgga gcatgacgaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc
     1141 gaaggacaac ctgaagtcca cgcagctgct gagtgtgatc gatgccatca gcgaagggcc
     1201 ggttgaaggt ccggtggatg gattaaaaag cgtgctgctg aacagtacgc cggtgctgga
     1261 cagtgagggg aataccaata tatccggcgt cacggtggtg ttccgggccg gtgagcagga
     1321 gcagtcaccg ccggagggat ttgaatcctc cggctccgag acggtgctgg gtacggaagt
     1381 gaaatatgac acgccgatca cccggaccat cacgtcggca aacatcgacc gtctgcgctt
     1441 taccttcggt gtacaggcac tggtggaaac cacctcaaag ggagacagga atccgtcgga
     1501 agttcgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac
     1561 cattaagggc aaaaccacct cgcagtatct ggcctcggtg gtggtggata acctgccgcc
     1621 gcgcccgttt aatatccgga tgcgcaggat gacgccggac agcaccacag accagctgct
     1681 gaacaaaacg ctctggtcgt catacaccga aatcatcgat gtgaaacagt gctacccgaa
     1741 cacggcactg gttggcgtgc aggtggactc ggagcagttc ggcagccagc aggtgagccg
     1801 taattatcat cttcgcgggc gcattctgca ggtgccgtcg aactataacc cgcagacgcg
     1861 gcaatacagc ggtatctggg acggaacgtt aaaaccggca tacagcaaca acatggcctg
     1921 gtgtctgtgg gatatgctga cccatccgcg ctacggcatg gggaaacgtc ttggtgcggc
     1981 ggatgtggat aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cggtgccgga
     2041 cggctttggc ggcacggagc cgcgcattac ctgtaatgcg tacctgacca cacagcgcaa
     2101 ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg
     2161 gcagacgctg acgttcgtgc aggaccgacc atc
//
LOCUS       DAOFSV010000305         2125 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.306, whole genome shotgun sequence.
ACCESSION   DAOFSV010000305 DAOFSV010000000
VERSION     DAOFSV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.306"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            48..272
                     /locus_tag="QBH27_004820"
     CDS             48..272
                     /locus_tag="QBH27_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDQ7017016.1"
                     /translation="MGPQMITYTTRDGDRLDQICLAVYGRASKTTETVLYQVSNYGVT
                     DMCAVFRAGEKIVLPDIEPEPVKKETQLWD"
     gene            276..1320
                     /locus_tag="QBH27_004821"
                     /pseudo
     CDS             276..1320
                     /locus_tag="QBH27_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
     gene            1487..1906
                     /locus_tag="QBH27_004822"
     CDS             1487..1906
                     /locus_tag="QBH27_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017017.1"
                     /translation="MVVKNGLACGIMLALVGCATKPVSNEQATPVPEKQIISTALLEK
                     RDGTGEVIIKRDSGFFGSACFSRVYVDGKEVADLGTAQKVVVYPTIGDHVFSAWPKGV
                     CGGGMSEQAGKVTTDRTLMYRIGYGSNGEYGIHPTAF"
BASE COUNT          594 a          445 c          556 g          530 t
ORIGIN      
        1 ctctccgact actaaaatta attctcatta tgaaatcctt ttaattaatg gggccccaaa
       61 tgattacata cactacacgg gacggtgacc gtctcgatca gatctgcctt gcggtctacg
      121 ggagagcgtc aaaaacgaca gagactgttt tatatcaggt ttcgaattac ggcgtaacgg
      181 atatgtgtgc cgtgtttcgt gctggagaaa aaattgtttt accagatatc gaaccagagc
      241 cggttaaaaa agaaacgcaa ttatgggatt gatgaatgag tgattacgta aatactggcg
      301 tggaggcgtg gaaacctaat ttttatatat ctgctgataa taaaaacatc acggataaaa
      361 taagaaaagg tttgatcaat atcacgctga cagattacgg cggctccagt aaacagacag
      421 acgaactccg cgtagcaata gtttctgaaa ctctgaaaat tccggcccgc ggagtaaaaa
      481 tcagtattgg gctcggtttc ggtaatcaga taattgataa agggatatac atcgttgacg
      541 gtgctagcag tggcggtgaa ccccgggtag ttgatttcac agcaaaagcg gctccgatga
      601 acgcagcaaa aggatctccg acagttcaga gcaaaaagac tcgttcctgg agcgacgtca
      661 ctgtcagcga tatcgttgcg acaatagcga gcgataatgg gctaaaacca cgcgtatcaa
      721 agcggtttgc agacaatgtt atcacgcaac tcgatcaggt cggcgagtca gatatgcacc
      781 tgatgtcgcg gctcgcaacg agattcgatg cggtgagtaa acccgcgggc gggtactgga
      841 tattcctgcc gcgcggcgcg ggcgaatctg ttagcggtgc tccgctccag cactacacgc
      901 taactcgtcg tgataactcc agctggggct attcgagaaa tggacaaagt ggcgatagtg
      961 gtagcggtgg cgaaaatccg gaaccgacgt atctgattaa atatcacgac acagcaactg
     1021 ggcagatcaa agaattgcgg acaaggtagt ggcggcgatc ctgtcattga atggccggca
     1081 gttgagccgt cgctcgacgc agcgaaagaa gctgcaccag ggctcaaggg cggtgcagct
     1141 aaaaaagagt tctctatgac gtacacgacg ccagcgacgc tcgatttagt gtcgctgaca
     1201 gcagaatgca aagtcacaac tcaaggtttc gggaccgagg aggaccggga ctggactatc
     1261 aacacactga gtctgacgct cgctgaaaac ggtttttcgg ttcggctgtc tctggagtaa
     1321 atgggctcgg cgagccggtg gcggtatgaa tttagtatta acagatgtcg actgcaaacg
     1381 gcacctgcga tatttttagg cgtaagccgg agaacgaaga gtctctggtg ctgaacgttg
     1441 ttatcgttag atgctgacat atttttaata ttgggagttt tcaaacatgg tcgttaaaaa
     1501 tggtcttgcc tgtgggatta tgcttgctct tgtcggttgt gctacaaaac ctgtctcaaa
     1561 tgaacaagcc actcctgttc ctgaaaaaca aattatcagt accgcgttac tcgaaaaacg
     1621 agacggtacg ggagaggtga tcatcaagcg tgactcgggt ttctttggaa gcgcgtgttt
     1681 ttcccgtgtt tatgttgatg gtaaagaggt ggcagattta ggtacagctc agaaagtcgt
     1741 tgtataccca acgattggtg atcatgtctt cagcgcgtgg ccaaaaggag tatgtggtgg
     1801 tggcatgagc gaacaagcgg gcaaggtgac aacagatcgt actttgatgt atcgcatagg
     1861 gtatggatca aacggtgaat atggaattca cccaaccgcg ttttaatttg tgttggggta
     1921 tgtgagtttt tctcataccc ctttacctcg ctttgctcct gacagtttgg tcacataaag
     1981 gcgcagaaca tcaccatttt tgccaaaaac cttcagaaga cgatgagtaa gacgcgtcgt
     2041 aactatatga ttatttgata ttcatacaca ccattatgtc cggataattt gccataatta
     2101 tttgaattta ttgcacaaag cgacc
//
LOCUS       DAOFSV010000306         2105 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.307, whole genome shotgun sequence.
ACCESSION   DAOFSV010000306 DAOFSV010000000
VERSION     DAOFSV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.307"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            59..1132
                     /locus_tag="QBH27_004823"
     CDS             59..1132
                     /locus_tag="QBH27_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDQ7017018.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTCHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            1129..1911
                     /locus_tag="QBH27_004824"
     CDS             1129..1911
                     /locus_tag="QBH27_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ7017019.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT          467 a          538 c          603 g          497 t
ORIGIN      
        1 gcgaataata aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt
       61 ggggcagcga atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca
      121 gcctggacga atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg
      181 cgtgctggat gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc
      241 tgccggggcg cagaacctct cggcgtttat ctgcaatcag cccggttacg cgcgcaaagt
      301 catcatgcgc tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg
      361 aaatcttatc gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat
      421 ggataccgcc gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca
      481 ggatgactac gcgcctaatt actttttacc aaccaaacaa aactggctgg atgtgattcg
      541 cgcctccagt gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta
      601 cctggatggc gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa
      661 aacgttggtc gtcatccgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa
      721 acgcatggaa cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca
      781 tcatgaaacc agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg
      841 gatattcgaa atttatccac cgaagccatt acatagtatc gcgcttggca gtcggattcc
      901 ggcgctgcgt gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt
      961 tggcaagcta ttaactgaaa aagcgccgct tacctgccat ctggtgccag tggtgacgcc
     1021 ggaatcgatc gttattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag
     1081 cgatgctccg caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt
     1141 tttatcgata cccactgcca ttttgatttc ccgccgttta gtggcgatga agaggccagc
     1201 ctgcaacgcg cggcacaagc gggcgtaggc aagatcattg ttccggcgac agaggcggaa
     1261 aattttgccc gcgtgcaggc gttagcggaa aaatatcaac cgctgtatgc cgcattgggg
     1321 ttgcatccgg ggatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg
     1381 ctggaaaggc gtccggcgaa ggtggtggcg gtgggggaga tcggtctgga tctcttcggc
     1441 gacgatccgc aatttgagag gcagcagtgg ttactcgacg aacagctgaa actggcgaaa
     1501 cgctacgatc tgccggtgat ccttcattcc cggcgtacac acgacaaact ggcgatgcat
     1561 cttaaacgtc acgatttacc gtgcactggc gtggttcacg gtttttccgg cagcctgcaa
     1621 caggccgagc ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat
     1681 ccacgcgcca gtaaaacccg cgatgtcatc gcaaaattac cgctggcatc gttgttgctg
     1741 gaaaccgacg cgccggatat gccgctcaac ggctttcagg ggcagcctaa ccgaccggaa
     1801 caggctgtgc gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt
     1861 gccgaagtgt tgcttaataa tacgtatgcg ttgtttagcg ttagtgggta gtttaccctt
     1921 cgtaggcagc atccggcagt cggcgcataa tgcctgatgc gacgcttgtc gcgtcttatc
     1981 atgcctacag gtttgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag
     2041 ttggcgcaca atgcctgatg cgacgcttga cgcgtcttat catgcctaca ggtttgtgcc
     2101 gaacc
//
LOCUS       DAOFSV010000307         2097 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.308, whole genome shotgun sequence.
ACCESSION   DAOFSV010000307 DAOFSV010000000
VERSION     DAOFSV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.308"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..421)
                     /gene="tolA"
                     /locus_tag="QBH27_004825"
     CDS             complement(<1..421)
                     /gene="tolA"
                     /locus_tag="QBH27_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDQ7017020.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAK"
     gene            complement(486..914)
                     /gene="tolR"
                     /locus_tag="QBH27_004826"
     CDS             complement(486..914)
                     /gene="tolR"
                     /locus_tag="QBH27_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDQ7017021.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(918..1610)
                     /gene="tolQ"
                     /locus_tag="QBH27_004827"
     CDS             complement(918..1610)
                     /gene="tolQ"
                     /locus_tag="QBH27_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDQ7017022.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(1607..2011)
                     /gene="ybgC"
                     /locus_tag="QBH27_004828"
     CDS             complement(1607..2011)
                     /gene="ybgC"
                     /locus_tag="QBH27_004828"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDQ7017023.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
BASE COUNT          499 a          584 c          477 g          537 t
ORIGIN      
        1 ctttcgctgc cgcctcttcc gcttgcttct gctttaactc ggcctgtttt gcggcttctt
       61 cagcctgctt tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt
      121 cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc ctgctgctct ttcatcttgc
      181 gctgctcatc agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa
      241 ctaccgcacc tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg
      301 aagcttctat attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca
      361 gcactgctga aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca
      421 ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca
      481 gatgtttaga taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag
      541 ttcagtgctt taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc
      601 gttttcgggt tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa
      661 cgctccaggc gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca
      721 atcactggcg gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg
      781 acctccacgc tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc
      841 agtacgtcca gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca
      901 cgcgctctgg ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc
      961 agaatcgcgg taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc
     1021 tggttgagac ggttataagc cattaccgcc ggaattgcgg cgaacaaacc aattgccgtc
     1081 gcaatcaacg cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc
     1141 ccgagggcga taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg
     1201 ctgatggagc caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacga
     1261 ttcatggaaa tacgcatcgc acgcgacgcc ccttccacta cggcttccgg cgcatggcta
     1321 ttggcacgat gcaggcgcac aaactctttg aagccgctgt aaaagatttg ttccgaaccg
     1381 ctcagattat cccgtttccc ctggctctct tgatagaggc gagagagttc gattccagac
     1441 cagaatttat cttcaaacgc ttcggcttcg cgcgccgctg cgttaagaat acgggtccgc
     1501 tggataataa tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta
     1561 accagaaggc tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact
     1621 ccgcgacaat agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa
     1681 ccagaacctc tgcttcattc aacaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa
     1741 ccaaagaggt gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc
     1801 gcgcaggtgc gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca
     1861 tcagcgcctg ttgactgaaa tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa
     1921 aagcgacgta actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc
     1981 gaaccggcca tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta
     2041 gagcttttaa acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaag
//
LOCUS       DAOFSV010000308         1951 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.309, whole genome shotgun sequence.
ACCESSION   DAOFSV010000308 DAOFSV010000000
VERSION     DAOFSV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.309"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..615)
                     /locus_tag="QBH27_004829"
     CDS             complement(<1..615)
                     /locus_tag="QBH27_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ7017024.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATSLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMG"
     gene            complement(612..1892)
                     /gene="wcaK"
                     /locus_tag="QBH27_004830"
     CDS             complement(612..1892)
                     /gene="wcaK"
                     /locus_tag="QBH27_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDQ7017025.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDKQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
BASE COUNT          427 a          499 c          543 g          482 t
ORIGIN      
        1 gcccatgcgc gatacggcga ttttttccct cgggcagccc attttttgca gccttccggc
       61 ccacagatcg cttattggta gcatcaggtc gccacggcga aacagttgct gatattcggg
      121 agtgtaatgg ttgagcacct cccgactgga gatatcaatg ccgtggaaga tagtggcaat
      181 tttgccgcga atgacaccca gttcgcggag ttttgctgcg gttacccccg cagggccaaa
      241 atgtgcgata aagacatcgg catgtaacga tgttgcgacc tgaccacaaa tggcagacaa
      301 aatcaggttc cgcgactctg cgccatagcg tttgaggttg agcgcctgcc aggtattttt
      361 acgatgaatg ccgcgtaagg tctggctggc tcggtggcgc agcttcgcca ctttgcccgt
      421 aggttcgtcc tgtaaccagc gggttctggc ggcaaggttg tatttcgtcc atgccgcgtg
      481 ggtgttttgt gtgtcgcctt tttgcaacgc gacaatctcc acctcaaagc ccatatcaat
      541 aaacgcagtg atttggttga ggacgaaggt ttccgacgac agcggaaatt tcaataaaaa
      601 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
      661 cctgtctgac gttcgcgact gacggcttcg ttaagtcgtg cgttcagcgc cggaagttgg
      721 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcta ataaatgacg gatatcaatc
      781 gccatctccg gtagtcccag ctgctgcatg atcccggcgg atttatgttc atagttgatg
      841 gcaatcgccg gggtggcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
      901 gtgagttcac aggcccccag aattttgccc atttccagat cgttgagttc atccatcacc
      961 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
     1021 tctttgttat agctgtcgat gcccgtacag gtggagagcg caatcacctg atagccttcg
     1081 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
     1141 aggcgtttgt cgaacggtgc cagttcacgc agggtaatgg ccaccgcttt ctgttgtgcg
     1201 gcaacgtcca gccagtgttg aacagcatag ctggcggtga agtcttctgt gtggtgatcg
     1261 accagccacg cggtatcgac gccatgttca acttttgcgg tagtgatatt gctgcgtttc
     1321 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaaaacgtag
     1381 ttcgccagtt ggttaaattg cttatcctgg aacgggccga cgctgtgacc aatcataaac
     1441 agcggctttt tcgccataaa cgtgcaaagc gcatgttcaa actgcggcac gccatagaga
     1501 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
     1561 aagtcagtga atccctgggc gatggcgata ttacgcaact tgccagtgtc ggtgacgcgt
     1621 gagagcagta cctgatgttg gtaacggcga cggaggactt ttttaacgcg cccgacaacg
     1681 cccgctgcac tgttgtgttg tttcatttgc aggaacagcg gatcgcccat taccgggcgg
     1741 ttgagcagcc aggaagaact gaccggatag cgactcatca cgtccacttc ggcatgtgga
     1801 ttgaggatat tgatggcatc aagtaagccg cgcaggatgg cgctgtcgcc gcgattgccg
     1861 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
     1921 aggctgtgtt tgttgttttc attgctgttt t
//
LOCUS       DAOFSV010000309         1944 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.310, whole genome shotgun sequence.
ACCESSION   DAOFSV010000309 DAOFSV010000000
VERSION     DAOFSV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.310"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(191..592)
                     /gene="pemK"
                     /locus_tag="QBH27_004831"
     CDS             complement(191..592)
                     /gene="pemK"
                     /locus_tag="QBH27_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HDQ7017026.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(525..782)
                     /gene="pemI"
                     /locus_tag="QBH27_004832"
     CDS             complement(525..782)
                     /gene="pemI"
                     /locus_tag="QBH27_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HDQ7017027.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(875..1528)
                     /locus_tag="QBH27_004833"
     CDS             complement(875..1528)
                     /locus_tag="QBH27_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDQ7017028.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          538 a          543 c          439 g          424 t
ORIGIN      
        1 acttcaaaac tgcgcaaaac acgcaaatac gcacaagaaa gtagggctgc gcttggaatt
       61 cggaaaataa accacatcaa aactactttt tagccaaata acgtttggga atcaccagaa
      121 tggtgggaca acagcggttt tagtgcccta aatcgtacgt tttcatccag ttgcccctca
      181 aaccccatgt tcaagtcaga atagtggaca ggcggccaag aacttcgttc atgatagtct
      241 ccggaacccg ttcgagtcgt tttccgcccc gtgctttcat atcaattgtc cggggttgat
      301 cgcaacgtac aacacctgtg gtacgtatgc caacaccatc caacgacacc gcaaagccgg
      361 cagtgcgggc aaaattgcct ccgctggtta cgggcacaac aacaggcagg cgggtcacgc
      421 gattaaaggc cgccggtgtg acaatcagca ccggccgcgt tccctgctgc tcatgacctg
      481 cggtaggatc aagcgagaca agccagattt cccctctttc catgtcagat ttcctcctga
      541 ccagtcgccg gtgcatccag ccattctcgt tcttcagctg atatttcagc attcggatca
      601 cactgtgcca gtagctcagc cagtgaatat tgcgggcgtc tgtacggctc aacaatcagc
      661 cggccattat caatgaccat gccaacttca ttatctgtgc ccagagacag cgcattcagc
      721 agtgccggtg ggacggtcag cataactgag ccgccaaccc tcttcagtcg ggtggtatgc
      781 attcttcacc tccataaaag ttatatttaa atataacatc cactaaaaaa acacaccagg
      841 ctttaacgca caatgtttaa taaaaatata actttcaacc aaacagtaaa cccagcgtgg
      901 ttgcttccat atgcagcaat accggcagca gcaggccacc acttctgatc ctggccactg
      961 atgtaatcaa ccccaccagg aacagttctg ccagtgtcag caggttctga tactggctgt
     1021 gcgcggcgac gaacaacaac gacgttatca gcgcccccag ccacatcgtc cagcagtacc
     1081 gtgaacggaa gacgttcagc ataatccccc ggaacagcgt ttcctcattc aacggggcaa
     1141 ggataaagat ggtcagcaac gtcaggatca cgtcaggtat ggacttatcg gcaaaaagtt
     1201 tcgtcataaa tggctcagca ggcagagcca gcgccttacc gagcagaaat acaccgacat
     1261 acaccacggc catcgcaccg accagccacg gtacgccaac gttgcgcagc tgaccaacga
     1321 ccggtagcgg agctatccaa cggcggtata ccaggaaaac acacagcagg tacatcagaa
     1381 cagtaccatg actgaagaac aaatagtttt ttcctgatcc ataaagcaga acggcctgct
     1441 ccatgacaaa tctggctccc caactaatgc cccatgcagc cagcataacc agcataaact
     1501 gcagatattg attacgtgtt tgaatcattg catcgcctgt aaatttttaa cttgtcctat
     1561 ttttgtcatt accacgtata tacacatgta taacaattca gatatcgtta ccaggatatg
     1621 ccgcatcagc ggcatggaag gcggcactct gttgtttcat atgatacagg agtaaaaccg
     1681 ccgaagcccg gcgtaagccg gtactgattg atagatttca ccttacccat ccccagccct
     1741 gccagaccat acccgctttc agccatgaga gagcttctgt gcgcggtcgg agtggtcccg
     1801 acgagggttt acccgaagtc ggggcgtatc tccgcgttag cgggccgtga gggccgctta
     1861 cgagcgtgta ctgagaactt ccagcgagaa gactgacagc gatgaagatg tagttacaac
     1921 attcataatt aaaagcgact ctgt
//
LOCUS       DAOFSV010000310         1884 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.311, whole genome shotgun sequence.
ACCESSION   DAOFSV010000310 DAOFSV010000000
VERSION     DAOFSV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.311"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            324..815
                     /locus_tag="QBH27_004834"
     CDS             324..815
                     /locus_tag="QBH27_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7017029.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     VSSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            818..1884
                     /locus_tag="QBH27_004835"
                     /pseudo
     CDS             818..1884
                     /locus_tag="QBH27_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
BASE COUNT          528 a          358 c          456 g          542 t
ORIGIN      
        1 acgttccctc cataaatctc ttgctattcc ttttataaaa agttcgcagt acccatacct
       61 ggcccccagc ctcgccgctg gtagttcgtg tcatgttttt tacgcgtaaa acacgacaaa
      121 agcatcaata cagcgatcgt ctcacttgta tgataataaa aacaatatgt tatatatgaa
      181 tgtgctgtta ttttattgtt tttatatata aaaagataaa atatcatcac tttcgtaatc
      241 ttgaacgctt tgcaggtgcg ttcaatccaa taaatgctaa tattatggct atcatagcca
      301 taaaaagata aaatattatc actatgggta aaaaaacagc accgttactc ccttatacga
      361 atcagatgct tagtgaattt ggtgagcggc tgaaacttgc tcgtttacga cgcaggctta
      421 ccgcaaagca agttgctgaa cgtgctggga tgtcgccaat aacattgcgc tctctggagt
      481 caggaagtgc aggggtcacg ataggggcat atttgtctgt gatgcaggtt ttgggcctgg
      541 aaacggattt aaataagctt gcagctgaag acgacttagg gcgtcagctc caggattccc
      601 gtttgattaa aaaaagaggg ctgtctcgtg tatcaagtaa gaatgaagaa ggttctcaga
      661 cagcctcatt gcagatgagg cacgagaaag aaggtcatct caccacggat ttaacagcac
      721 tggcagaagg ctcgacgcca tctattacag gagaaacatc aacctctctt gctgcactat
      781 taaaaccgat aaaaaaaccg ttaaaaggaa attaattatg gcattaatcg gagtgtatgc
      841 ggattgggaa ggcctggatg gtcctgcgcg tattggttat ttacacagtc gtcgcactcg
      901 ggcgcgtgag atatttgagt ttgagtacga taaaaaggcg cttgcagatc cttcacttaa
      961 tttcattcag ttagatcctg aaataatgtt gtatgaggga gcacagtacc caattcctcc
     1021 aaaggataaa tttggtgcat ttagtgattc ctgcccggat cgttgggggc gtatgctaat
     1081 gaagcgtcgt tttgagaggg atattcgaga cgggttatgt gataaagact cccaccttta
     1141 tgagtctgat tatctactag gtgttcatga tctttatcgg gttggggctc ttcgatacaa
     1201 acgtgaagac gctggagagt ttcttgataa tcgcattgat gttgcagccc caccatttac
     1261 cgaaatagcg agtctggaaa gggttagccg ggcaattgaa gaagatcctg ataataaaga
     1321 gtaatggggc aaaaatggtt aagaatgcta atcgctccgg ggggatcatt agggggggca
     1381 cgtccgaaag ccagtgttgt tgatgaagct gggcatctct acatcgctaa atttcccagt
     1441 gtaaaagatg agtatgatgt tggtggatgg gaaatggtcg ttaatgcact tgctgttggc
     1501 tgtggactga atgttgcacc tgctcaggcg cataaatttg ccagcaatta ccattgtttt
     1561 atggttcgtc gttttgaccg cactaatgcg ggaaggcgtc tgcattttgc ttcggctatg
     1621 acactaacca ggcatcagga tggtgaagat gcctctacag gtgtgagtta tctggaatta
     1681 gcagatgttc ttatcagaca tggcgcgcag actaatactg accttaagga actctggtcc
     1741 aggattgttt ttaatattct tgttactaat accgatgatc atttacgcaa tcatggattt
     1801 atcttgatac caggaaaagg ttggcggctt tctgaggcat atgatttgaa tcctgtggtc
     1861 aggtctgatg gtctaaaact caat
//
LOCUS       DAOFSV010000311         1796 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.312, whole genome shotgun sequence.
ACCESSION   DAOFSV010000311 DAOFSV010000000
VERSION     DAOFSV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.312"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(154..1641)
                     /gene="yeeO"
                     /locus_tag="QBH27_004836"
     CDS             complement(154..1641)
                     /gene="yeeO"
                     /locus_tag="QBH27_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDQ7017030.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALA
                     LTYLELTVFSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     AGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          561 a          490 c          370 g          375 t
ORIGIN      
        1 ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga ggaatcgtga
       61 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
      121 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      181 acacttttgc ggctcggatc gagggtattt ccatagccaa cgtccagtaa ccattcgcca
      241 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      301 accccagcca agcatgattc ccagcacata accgactaca acccgacaac cccacatgct
      361 caacatcgaa acccacatgg cgtaacgggc atcacgagca cctttaaatc cagcgggtag
      421 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      481 gacgtgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      541 cgttagccag gcgatggccg ttaatccaag agtggacagc cagaacacat gccgcaactg
      601 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      661 agaaccgagc gcacttccgg gtaagttgat aagagccgca attgagaacg cgataaaatt
      721 tccggcaata acgctggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      781 ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt cccagataat
      841 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      901 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccaa
      961 cccagccccg acaaatccca gtcccgccca ggagaaaagg ccgtaaatca atatgccgct
     1021 aataataata ttaagaatat tcaggctacc gttaatcagt agcggtattt tcgtattccc
     1081 tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat aactgaatac
     1141 ggtcagctcc agataagtca acgccagtgc tttaacgtct gtcgtggcat cacccgcgac
     1201 gaaatcaata atttgttcgc caaaatgatg aattagcgtt gccaacagta cggcaaacaa
     1261 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat ctcgcttacc
     1321 gagactaaat gctacaacga cagtagtacc aagatcgata gctgcaaaaa aagccataat
     1381 gaccatattg aagctgtccg ccaaccccac gccggccatc gcatcttttc ccagccagct
     1441 gaccagaaaa gtgctcagga cccccatcaa caggacacag gcgttctcca tgaagatagg
     1501 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagagtg
     1561 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1621 ttgcagaaag tgaaaccaca tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt
     1681 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1741 aaatttaaaa atcgggtttg acaaaatatt tttcgccgtt aagatgtgcc tcaaca
//
LOCUS       DAOFSV010000312         1780 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.313, whole genome shotgun sequence.
ACCESSION   DAOFSV010000312 DAOFSV010000000
VERSION     DAOFSV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.313"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(224..566)
                     /locus_tag="QBH27_004837"
                     /pseudo
     CDS             complement(224..566)
                     /locus_tag="QBH27_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(601..720)
                     /locus_tag="QBH27_004838"
     CDS             complement(601..720)
                     /locus_tag="QBH27_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDQ7017031.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            complement(681..865)
                     /locus_tag="QBH27_004839"
                     /pseudo
     CDS             complement(681..865)
                     /locus_tag="QBH27_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            966..1139
                     /locus_tag="QBH27_004840"
     CDS             966..1139
                     /locus_tag="QBH27_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017032.1"
                     /translation="MSYFSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDN"
     gene            1141..1485
                     /locus_tag="QBH27_004841"
     CDS             1141..1485
                     /locus_tag="QBH27_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDQ7017033.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            1489..>1780
                     /locus_tag="QBH27_004842"
     CDS             1489..>1780
                     /locus_tag="QBH27_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDQ7017034.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLL"
BASE COUNT          511 a          396 c          394 g          479 t
ORIGIN      
        1 gctgttccca cgcacagaac aaaatgttta cgcctggttg agtaaataac cttattgtta
       61 gcatgggtat ccagggagat gataacccgc aatttcatta ttcattgttc tccaatagga
      121 tgaggtttcc cgccgtaact ggcgggcttt ttttgcccca gaattttccc ttttagcacc
      181 ctgtaaacga aaacagcgcc gaaacgcagt taatcgttga tggtcagaac ggccagttca
      241 caccggccac cgcattccac ggggactcca caccggcacc acggttatac ccaccccgag
      301 atgcccgctt aacatactgc tgaatgaggc tttaatacct gcctggtata ttccacgtct
      361 gcccgacaaa tcattgacaa aattaccgtc actattcacc ttcacccggt tatcatcgac
      421 aaattctttg cgcacagccg ccttcagcca gggttcaatt tccataccgt tccccagacg
      481 catgttgtag ctcagcgttg cgcccagttc acgatatata ctgcgggtat cgactgattt
      541 cgattccatg cattggataa atgatagtag cgtctaagtg accttagctc tgctgcaaca
      601 tcagcccgca ggcaccagac caggggattc atcctgaaga gacagcgcaa atgtattgtg
      661 ttcaccgctc atcaaagaca tcatgatgaa atgatgatat cccgtataag aaagaggcat
      721 tttttaaacg cagtgcgctg aagtgttgtt ggataaaaaa cactcttttc tgataatact
      781 gtcgtacgct tatttttcga caatgaggcc gtccacaaaa taaaatgact aatgtaatcg
      841 ttgctaatga atatcttctg ctcacataac aatcacacaa taatattgat aaatcagatt
      901 atcttacctt tacaacattg tattaacagc caatgacact tcttgaataa aaatattgtc
      961 aatcaatgag ttatttctca tttgagatta ttctcattcc cgtaaaaaac ataattccta
     1021 tcatcacagt cacgctcatt ttgaattatc tgaataatag cgagcgttct ttagttaagc
     1081 agatcttaat tgaagacgaa attattgtgt gtgcaactta cttaataccg gacaactaag
     1141 ttgaagaaaa aaatactcgc cttcgggctt atctcagcat tgttctgctc cacgccagca
     1201 atggcggata tgaatcgcac cacgaaaggt gcattgctag gtgcaggcgt tggtttactt
     1261 actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg ttggcgctgg tgttggtgca
     1321 gtaacagaaa aaggccgtga cggtaaaaat gcgcgtaaag gtgcaaaagt gggtgccgcc
     1381 gttggtgctg tcactggcgt tctgaccggc aatggcctcg aaggggcgat taaaggtgcc
     1441 gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga aataaggaat gatgatgaaa
     1501 aaatttgctt tactggcggg tctgtttgtt ttcgcaccaa tgacctgggc gcaggactac
     1561 aatattaaga acggtttgcc atcagaaacc tatattactt gcgcagaagc caacgaaatg
     1621 gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg taatgggtaa tgccagcgtt
     1681 gccagccgtg atttaaaaat tgagcaatct ccggaactga gcgccaaagt ggtagaaaaa
     1741 ctgaatcagg tttgcgctaa agatccgcaa atgctgttga
//
LOCUS       DAOFSV010000313         1740 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.314, whole genome shotgun sequence.
ACCESSION   DAOFSV010000313 DAOFSV010000000
VERSION     DAOFSV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.314"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..156
                     /locus_tag="QBH27_004843"
     CDS             <1..156
                     /locus_tag="QBH27_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017035.1"
                     /translation="NHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFS
                     FAEHGLL"
     gene            219..440
                     /locus_tag="QBH27_004844"
     CDS             219..440
                     /locus_tag="QBH27_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDQ7017036.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            459..1103
                     /locus_tag="QBH27_004845"
     CDS             459..1103
                     /locus_tag="QBH27_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDQ7017037.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
     gene            1153..1521
                     /locus_tag="QBH27_004846"
     CDS             1153..1521
                     /locus_tag="QBH27_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDQ7017038.1"
                     /translation="MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCDYVYLAVYPTPEMKN"
     gene            1610..>1740
                     /locus_tag="QBH27_004847"
     CDS             1610..>1740
                     /locus_tag="QBH27_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDQ7017039.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     "
BASE COUNT          389 a          515 c          446 g          390 t
ORIGIN      
        1 aatcacccgt ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg
       61 gtacaggcac tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac
      121 caggttttct cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg
      181 ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaattatc actcgtggtg
      241 aagccatgcg tattcaccaa caacatccga catcccgtct tttcccgttc tgtaccggca
      301 aataccgctg gcacggcagc actgaagcgt ataccggtcg tgaagtgcag gatattcccg
      361 gtgtgttggc cgtgtttgct gaacgtcgta aggacagttt tggcccgtat gtccggctga
      421 tgagcgtcac cctgaactga atcaggacgg gcattcagat gagcagcatt atcaccatca
      481 cagtgtatac gctggatgaa ctttcctgcc cggcacgggg aaaagcccgc gactggtacc
      541 gtcagcatca tgctgacagc aactggtatg aaaacgtgta tgaagacttc agggaggtct
      601 gtggcatttt cgggattgac ctccgccagc gcgttttccg gctcagcaat ggcaggttta
      661 tggaagagcc gtgcatctgg ttcagcggat tctgcagcca gggggacgga gcctgttttg
      721 aagggcgctg gcactggcag ccagcgacac cccggaaaat cagggaatat gcccctcagg
      781 accgtgagct tcatcgtatc gcggataccc tgcaggcggt gcagaaacgt aacttctggc
      841 agcttcaggc tgaaatcagt caccggggac gttactgcca tccgtacagc atggatatca
      901 ccgtcacccg gaacagtccg actggtcagg caatgacaac cgatgcggaa gccgctgtca
      961 gcgaagccct gcgcgacctg gccttctggc tgtaccgcca gctggaaaac aagtacgact
     1021 ggctgacctc agacaccgct gtggatgagg cccttctcat caacgaatat accttcaccg
     1081 aagccggtct gcgcgccggc tgacccgtca tcgttaaact atcattcacc gattttctgt
     1141 gaggatttta tcatgtcgga cacactcccc ggaacaacgc ttcccgacga caacaaagac
     1201 ctcccctggt gggggctgcc ctgcaccgtg acgccctgtt tcggggcctg tctggtgcag
     1261 gagggtaacc ggttgcatta ccttgccgac cgcgccggta tcagaggccg gttcagcgat
     1321 gcggatgcgt accatccgga ccaggccttt ccgctgctga tgaaacaacc ggaactcatg
     1381 ctcaccagcg gtgaactgaa tccccgccat cagcataccg tcacactgta tgcgaaagga
     1441 ctgacctgcg aagccgatac cctgggctcc tgtgattacg tttatctggc tgtttatccg
     1501 acgcccgaaa tgaaaaatta actctccaga atagccttct gctacggcct ggtgttttca
     1561 ccacgccact tttccatttt tatatctgca tatcaggaaa atcttcagta tgaaaacatt
     1621 acccgacaca cacgtacggg cggcatcgcg ctgcccgtct cccgtcacca tctggcagac
     1681 actgctcacc cgactgctgg accagcacta tggcctcacg ctgaatgaca caccgttcgc
//
LOCUS       DAOFSV010000314         1729 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.315, whole genome shotgun sequence.
ACCESSION   DAOFSV010000314 DAOFSV010000000
VERSION     DAOFSV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.315"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..273)
                     /locus_tag="QBH27_004848"
     CDS             complement(<1..273)
                     /locus_tag="QBH27_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7017040.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVT"
     gene            complement(273..1436)
                     /gene="emrK"
                     /locus_tag="QBH27_004849"
     CDS             complement(273..1436)
                     /gene="emrK"
                     /locus_tag="QBH27_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDQ7017041.1"
                     /translation="MEQINSNKKHSDRRKYFALLAVVLFIALSGAYAYWSMELKDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT          483 a          362 c          342 g          542 t
ORIGIN      
        1 ggttactgaa agtaaaaata accttaattc gcctattctt tgtgccagcc tgccggtaac
       61 agggatcgca atggcatttg ctacgccaaa cgaggtgata acccaggtac cttcgtctgt
      121 tgatgctccc agaaagccag atattgtcgg tattgcgacg ttagaaatag tggaatccaa
      181 catttgcata aacgtcgcta atgacaatgc aatagtgacg caccataacg tcccaccggt
      241 taatggtgca ggagttgatt tagtgattgc cattaaagtt gtccattatg cgaaataatg
      301 ttgctaattt ctttttctat cggactggta tcgataacta aagccttact ggtataagca
      361 ggcatggacg tcacggttga agccagatcg ggcatctcgg caatgtcttc attcttcgta
      421 tcaatagttg cagtcatcga taaaccaatg cgcaaagggt gttccatgag ttcttttgga
      481 tccagagaaa cttcaaccgg tacacgctga acgattttga tccagttccc tgtcgcattt
      541 tgtgcaggta ataaggagaa cgcattgccg gttcccatat tgatccctgt cacccgacca
      601 tgaaacacaa cattttcacc ataaagatcg ctgataatat tgaccgattg accaatccgt
      661 acatccgtga gttgtgtttc tttaaagttg gcattaaccc acatttgacg cgccggtacg
      721 acagccatta acgattgtcc gggactcact gtttcgccga cctgaacact tctctgggca
      781 atataaccgg taaccggact cttaatatcc gtacgtttaa gcgccaacca ggcttctttg
      841 gttgcatctg cagcttcaat gacttgtggc tgacggttta atggtgtgtt cattactaaa
      901 gctttattcg ctttataagc ctggatagca gcattcaatg ccgctttgct acttattaac
      961 gtatctttgg tatgctccag cgcttctttt gaaataaccc cttgcttcgc taaaggcaca
     1021 cgacggttat aatcttctaa agattgttga tactgaatac gtgctgaagc gacttcggca
     1081 ctgtattgtt tatcctgtaa gtatagttta ttcgtttgcc gaacaatatt tgccagatta
     1141 tttttagctt tattgagtgc gatagtggca tcagttttat ccagtgaaac taaaatgtca
     1201 ccttgtcgaa cgtagttcgt atctttatga ttaacgacag tgacactgcc tgagacttgt
     1261 gcagaaattg gatctgcatt tcccgtgaca taggcgtcat ctgtactaat catgtctttt
     1321 aattccattg accaataggc ataggcacct gaaagcgcaa taaataaaac taccgctaac
     1381 aaagcaaaat attttcttct gtcagaatgt tttttatttg aattaatctg ttccactatt
     1441 atctctcatt tcgcatagat gaaatttatg aattgaatga aaagagtatt cattcctcat
     1501 atcaaacaca gtgcatgttt tgcacattat cccaggaacg ttggagggct aatttaaatg
     1561 aggtgcaaaa aaaacaacag aggactaaac cgcggctttt gcaatacaat tcttacgcct
     1621 gtaggattag tgagaaaact tatagtgctc atttgaaact ataaatcatc ggtatcatcc
     1681 ctgattttat tgttgacatt ttatttatgc cgactattta tatggtata
//
LOCUS       DAOFSV010000315         1685 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.317, whole genome shotgun sequence.
ACCESSION   DAOFSV010000315 DAOFSV010000000
VERSION     DAOFSV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.317"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1655
                     /locus_tag="QBH27_004850"
     rRNA            <1..1655
                     /locus_tag="QBH27_004850"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          425 a          373 c          532 g          355 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcgaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg
      481 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      541 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      601 taattgatgg ggttagcggt aacgcgaagc tcttgatcga agccccggta aacggcggcc
      661 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga
      721 atggcgtaat gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg
      781 aagatgcagt gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac
      841 actgaacatt gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt
      901 ctgcatggag ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtgggcccc
      961 ttaccggggt tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag
     1021 agtaacggag gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat
     1081 ggcataagcc agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag
     1141 tgatccggtg gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat
     1201 aacaggctga taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg
     1261 ctcatcacat cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg
     1321 gtacgcgagc tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct
     1381 ggagaactga ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt
     1441 tcgggttgtc atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa
     1501 agcatctaag cacgaaactt gccccgagat gagttctccc tgactccttg agagtcctga
     1561 aggaacgttg aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag
     1621 ctaaccggta ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga
     1681 agaga
//
LOCUS       DAOFSV010000316         1679 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.318, whole genome shotgun sequence.
ACCESSION   DAOFSV010000316 DAOFSV010000000
VERSION     DAOFSV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.318"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(7..474)
                     /gene="slyB"
                     /locus_tag="QBH27_004851"
     CDS             complement(7..474)
                     /gene="slyB"
                     /locus_tag="QBH27_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDQ7017042.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            748..>1679
                     /gene="anmK"
                     /locus_tag="QBH27_004852"
     CDS             748..>1679
                     /gene="anmK"
                     /locus_tag="QBH27_004852"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDQ7017043.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALL"
BASE COUNT          416 a          426 c          420 g          417 t
ORIGIN      
        1 acttatttag cgcggggaaa cggtcacctg actgccattg ctggccagta cgacacgttg
       61 gcctggagag aaatgagtgt tcccttgttt ctgtaccacc atgatggtgt tgccatcatc
      121 tttacgaatt tccagctcga caccctgcgt tttgttcatt gcactctgta cgccctgacc
      181 ggctacacca cctgcaacgg cccctgctgc agtagccaga gaacgcccgg ttccgccacc
      241 aatagtattt cccaggaaac caccaagtac agcaccgcca attgcgccga taacgttgga
      301 atcatcaccg ccctgaatct gtacctgacg tacattaacg atggtgccat agctgacatt
      361 ctgtacttgt ttcgcttcag aagcggtata aacatcccct gacagggtgt cgttattaac
      421 acaaccgaca agagacagac ctaccattga aacaaccaat acgcgtttaa tcattgaaaa
      481 atctcctgtt caccatgaaa cgccacgcaa gcatccctca tggtcaaagt atatggcata
      541 tgtgaatcca tggttacatg ttctaccaaa tatgagcaaa tcataatgaa agtaggatca
      601 accaattata aacaaaatat tcatgatgat tattgatttg aagataatgt tttactacca
      661 acggtaaaaa ttggtcattc cgcgtggtat tcacgaaaag tttatgtttg agtggtgcgc
      721 aaacgtcaga ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca
      781 ggcaccagcc ttgatggtgt tgatgttgtg ttagcgacaa ttgatgaaca ccgggtcgca
      841 cagctggcaa gtttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt
      901 tgccagggcc agcagcttac actttcgcag tttggacagc ttgatactca actcgggcga
      961 ctttttgctg atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatatc
     1021 gttgcgatcg gctgtcacgg tcaaaccgtc tggcacgaac cgacgggcgt ggcaccacac
     1081 actttacaga ttggcgataa caatcaaatt gtggcgcgca ccggaattac ggttgtcggt
     1141 gattttcgcc gtcgcgatat tgccctggga ggacaaggtg caccgctggt acctgcgttc
     1201 catcatgcgc tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc
     1261 gccaatctgt cactgctcat tcctggccag ccggttgggg gctacgatac cggtcctggt
     1321 aacatgctga tggatgcctg gatctggcgt caggccggta aaccttacga taaagatgcc
     1381 gagtgggcac gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgaccca
     1441 tatttctcgc aacccgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg
     1501 gagcgccatt tgcgccattt tccgggggtt gatccccgtg atgtgcaggc cacagtggca
     1561 gaactcaccg ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg
     1621 atggtatgtg gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc ggcattact
//
LOCUS       DAOFSV010000317         1672 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.319, whole genome shotgun sequence.
ACCESSION   DAOFSV010000317 DAOFSV010000000
VERSION     DAOFSV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.319"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..712)
                     /locus_tag="QBH27_004853"
     CDS             complement(<1..712)
                     /locus_tag="QBH27_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7017044.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTIFLTSVLAL"
     gene            complement(709..>1672)
                     /locus_tag="QBH27_004854"
     CDS             complement(709..>1672)
                     /locus_tag="QBH27_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ7017045.1"
                     /translation="FFIWWVSKGRFIQTTDDAYIGGNITTVTSKVSGYISAIEVRDNQ
                     SVKKGDIILRLDDRDYRANVARLEAKIKSSKANLESIQATIAMQQSIIQSASETWQAV
                     KHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQQVAAKERKAANDFLVEKQRLAV
                     LSAQEENVRASIEEVQAALTQALLDLEYTLVRAPIDGIVANRSAHTGSWVEGGTSLVS
                     LVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIP
                     IENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT          471 a          423 c          335 g          443 t
ORIGIN      
        1 ccaatgccag aactgatgtc agaaaaatag tattatcatc cagccatccc cagcgcgctc
       61 cttcttcaag ggtatattcc agacatccca gtgtcatggc caacaggata atactcggat
      121 aatcagcaac tttcagtaaa gagagatcag gtttatcaaa attaaccaga aaaggaatac
      181 tcaaaactaa atatatgcca ggtaatatat tgatataaaa tagccatcgc cagtcgaggt
      241 tatcagtgat ccagcccccc agagttggcc ctagcgtcgg tgacagtgag gccagcgcac
      301 tgactaccgc agcagccaac cccaactctt taccctggta ataaataaac gccgtcgtga
      361 aaactaaagg gatcatggat gcgccagcag ctccctgtaa agcacgaaaa aaaatcatga
      421 tctgaatatt ccaagcaagt ccacacgcga tactcattaa agtaaatatt cccgcggata
      481 gagtaaaaag ccaacgagtg gaaaataccc gtgacagcca cccagagagc ggaataacaa
      541 taatctcagc aattaaatag ctggtctgaa gccaggccat ttcgtcttta cctgcggaca
      601 gaccaccacc tatttcattt agggatgacg atacaatttg aatatcaagc tgagcaataa
      661 ataatcccat acacatactg gcgaaagcaa tatgtttacg catagacatc atctctcatc
      721 aacactgacg gtgacggaca gtcccgggcg aagaagttgc tttaattcct ttgcgtcgtc
      781 aaatgcaatc cgtaccggga cacgctgaac aattttggtg aaattgccag tggcattttc
      841 tatcggaatc aagctgaaac tggctcctgt cgctggggaa aggctttcaa tgtgaccgtg
      901 aaacacttcc cctttgagaa tatcagcacg gatctcagcc ttcattcctg gcttcattcc
      961 cgctatttgg ttttctttat aatttgcatc aacccacaac tcactgactg gtactagtga
     1021 aactaacgat gtgccgcctt ccacccaact gcctgtatgg gcgctgcggt ttgcgacaat
     1081 gccatcaatc ggcgcacgaa caagggtata ttccagatcc agtagtgcct gagtcaaagc
     1141 ggcctgaact tcttcgatac ttgcacgtac gttttcttcc tgagcagata aaactgccag
     1201 ccgttgcttc tccaccagga aatcgttcgc ggctttgcgc tcctttgctg cgacctgctg
     1261 gtagtcaaac ctggcgttat caataatttg ttgcgatatt gccgctgatt gtgcgagttt
     1321 ttcataacgt tcagtatctc gcaggcgctt ttgctcctca tgttttactg cctgccatgt
     1381 ttcactggca ctttgaatta tcgactgctg catcgcaata gtggcttgaa tgctttcgag
     1441 attggctttg ctacttttta tttttgcctc aagcctggcg acatttgcac gataatcccg
     1501 atcatcgaga cgcaggataa tatcgccttt ttttacactc tgattatcgc gcacctctat
     1561 agcagaaata taaccagaaa ctttacttgt caccgtggtg atattaccac caatgtaagc
     1621 atcatccgtc gtctgtataa atcttccttt gcttacccac cagataaaaa ag
//
LOCUS       DAOFSV010000318         1671 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.320, whole genome shotgun sequence.
ACCESSION   DAOFSV010000318 DAOFSV010000000
VERSION     DAOFSV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.320"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..773)
                     /locus_tag="QBH27_004855"
     CDS             complement(<1..773)
                     /locus_tag="QBH27_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDQ7017046.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPVVEQNLMPWLATGAATALITALAIVELNDDDDHHHRNNSP
                     LPPTPPDDNSDDTPVPPTPGGDEIIPDDGSDDTPTPPKPISFNNDVILDKTAKTLTIR
                     DSVFTYTENADGTISLQDSNGRKATINIWQIDEANNTVALDGVSADGATKWQYNHNGE
                     LVITGDNATVNNNGKTIVDGKDSTGTEIAGNNGKVIQDG"
     gene            complement(1101..1429)
                     /locus_tag="QBH27_004856"
                     /pseudo
     CDS             complement(1101..1429)
                     /locus_tag="QBH27_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="involved in the synthesis of a cofactor required
                     for carnitine dehydratase and carnitine racemase
                     activities; frameshifted; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
     gene            1437..1671
                     /locus_tag="QBH27_004857"
                     /pseudo
     CDS             1437..1671
                     /locus_tag="QBH27_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          454 a          367 c          357 g          493 t
ORIGIN      
        1 ataccgtcct gaataacttt cccgttatta ccggcgatct ccgtgccagt ggaatcttta
       61 ccatcaacga tggttttgcc attgttgttt actgtggcat tatcacccgt aataacaagc
      121 tcaccgttat gattatattg ccacttcgtt gcgccatctg cgctcacccc gtcaagggca
      181 accgtgttat tcgcttcatc aatttgccaa atattaatag ttgccttacg gccattgcta
      241 tcttgtagag aaatagtccc gtcagcattc tcggtataag taaaaactga atcgcgaata
      301 gttaacgttt ttgctgtttt atcgaggata acgtcattat taaacgaaat tggtttggga
      361 ggcgtcgggg tatcatccga accgtcgtct ggtattatct catctccgcc aggagttggg
      421 ggaactggag tgtcgtctga attatcatca ggtggagttg gtggaagtgg agaattattg
      481 cgatgatgat gatcatcatc gtcgtttagt tccactatgg ctaaggctgt gattaacgca
      541 gtagcagcac ctgtggcaag ccatggcatc aggttctgtt cgactacagg ggagcattca
      601 gagggtaagc gtttaagctc ttctggactg agttttccag ggtctgccgg acaagtcaca
      661 ttggtatttt tcttttcacc tgtggtggtt tctatctctg tgatatctgc cgcccagccc
      721 tgaccactca gagccaatgc aatacaggct gacaatagag ttttcctttg cataataaat
      781 ccttctggcg aatataaaga aggtcggatg ccttaaccca accaattaat caatcaatta
      841 aaacatagag gacattcaat attgccagta gattggcacc taatcaaaca tattctctga
      901 caatctgtaa ttataatttg agtgaatgaa tacacctgca taatatacaa cttgcgtata
      961 ttatatgaat tatagattta aatggcatca gataagcatt catttgataa aaaacggtac
     1021 aaatcaatac tcaaataaat aaatcagagc agaagcataa ttctgcttta taattaaagg
     1081 gaaaaataat tatataactg ttactgtttc ggtcgcagat tctcatcaaa caccaggcgt
     1141 ttcctgccag gttcaacttc ccgcaatggc tcgacttgat gcaacgtctg cttacaacga
     1201 gtcaccagca cctgatgccc tgctttctca atgccagctc ctgctcgctg agttcacgaa
     1261 tcgctttcgc cgggctgctg acaatcaagt aattagtcgg catttctgct ttggctttca
     1321 caaatgcgga aacaccaaca atactgttct caccaatcac cacaccgtcc atcactaccg
     1381 cgttaattcc cactaatgta tggcggtggt taatgcagcc gtgaagaata gtcggagata
     1441 caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg gtggtcgcgc
     1501 aatatattcg taaccgtcgt ctcgattttt gtgcagatgc gattcgccat gccgcagatg
     1561 atgaaaaact ggcaggcatt ggctttcatt ggggattttc cgaccagagt catttttcaa
     1621 cggtatttaa acaacgcttt gggatgacgc caggcgagaa tcggcgtaaa t
//
LOCUS       DAOFSV010000319         1626 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.322, whole genome shotgun sequence.
ACCESSION   DAOFSV010000319 DAOFSV010000000
VERSION     DAOFSV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.322"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            104..1057
                     /gene="ompT"
                     /locus_tag="QBH27_004858"
     CDS             104..1057
                     /gene="ompT"
                     /locus_tag="QBH27_004858"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDQ7017047.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1289..>1626
                     /locus_tag="QBH27_004859"
     CDS             1289..>1626
                     /locus_tag="QBH27_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDQ7017048.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPA"
BASE COUNT          522 a          333 c          337 g          434 t
ORIGIN      
        1 tgttaaacaa aatataaaca gtggggcaat atacaattga ctcattaagt tagatataaa
       61 aaatacatac tcaatcatta aaacgattga atggagacct tttatgcggg cgaaacttct
      121 gggaatagtc ctgacaaccc ctattgcgat cagctctttt gcttctaccg agactttatc
      181 gtttactcct gacaacataa atgcggacat tagtcttgga actctgagcg gaaaaacaaa
      241 agagcgtgtt tatctggccg aagaaggagg ccgaaaagtc agtcaacttg actggaaatt
      301 caataacgct gcaattatta aaggggcaat taattgggat ttgatgcccc agatatctat
      361 cggggctgct ggctggacaa cactcggcag ccgaggtggc aatatggtcg atcaggactg
      421 gatggattcc agtaaccccg gaacctggac ggatgaaagt agacaccctg atacacaact
      481 caattatgcc aacgaatttg atctgaatat caaaggctgg ctcctcaacg aacccaatta
      541 ccgcctggga ctcatggccg gatatcagga aagccgttat agctttacag ccagaggtgg
      601 ttcctatatc tacagttctg aggagggatt cagagatgat atcggctcct tcccgaatgg
      661 agaaagagca atcggctaca aacaacgttt taaaatgccc tacattggct tgactggaag
      721 ttatcgttat gaagattttg agctaggtgg cacatttaaa tacagcggct gggtggaagc
      781 atctgataac gatgagcact atgacccagg aaaaagaatc acttatcgca gtaaagtcaa
      841 agaccaaaat tactattctg ttgcagtcaa tgcaggttat tacgtaacac ctaacgcaaa
      901 agtttatatt gaaggcgcat ggaatcgggt tacgaataaa aaaggtaata cttcacttta
      961 tgatcacaat gataacactt cagactacag caaaaatggt gcaggcatag aaaactataa
     1021 cttcatcact actgctggtc ttaagtacac cttttaacaa cgttaaatag attttctccc
     1081 cggtggtatt gtatagcccc ggggaatttg tttaccgtta aaagcaaaca atatccccta
     1141 atcttgtaag catttaacct gcgtcctggc gtgtaacctc ttaccgcaac acaagactca
     1201 atatattcaa attcgttatg ccgtggcatc atttaacccc ttgtaattca tcgctataat
     1261 tgatttaatt cacaaataaa actataacat ggtgaaacta atgaaaaaaa acacagatga
     1321 tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg ttttgggtgt
     1381 atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac actttctgga
     1441 acatttaggt aacaaccatc tggatattgg cgttggaact gggttttacc ttactcacgt
     1501 acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga acgcggcatc
     1561 gacaagggct ggggaatcaa aaattaaaca taaaattagc catgatgttt ttgatcctta
     1621 tcccgc
//
LOCUS       DAOFSV010000320         1571 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.324, whole genome shotgun sequence.
ACCESSION   DAOFSV010000320 DAOFSV010000000
VERSION     DAOFSV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.324"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            695..1525
                     /locus_tag="QBH27_004860"
     CDS             695..1525
                     /locus_tag="QBH27_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ7017049.1"
                     /translation="MNSQNALSNMRLAVRGDISGNSTLVLKKIRFYNCAIIYLRNAQL
                     LVKTIDGGVINIPPESICYIEKNTVIDVTLNVLGKGMPYDIFHIDNNILSCICKVMEP
                     LLLNPHKIGPMRSRIFNCMADKTDTEIFKMITEANVPNHRLIYNITYLLSKVDDIESL
                     VCSLSVSTDSTFTEKLKLIIESDLSKSWRLVDLANVLHMSEVSIRKKLEKENNNFNNL
                     ILDIRMQHAARLITTTEKHINSISDEVGYVSTSYFIRNFKSYFGITPKQFSLKVKRKS
                     "
BASE COUNT          544 a          211 c          268 g          548 t
ORIGIN      
        1 gctatgaccg ttgcgctaat aatggttaca aaagattttt atttaaaaaa tggtctctta
       61 tatttttcaa gtcaggatga agagtataaa tgttttgagt tcagtggccg tgcctctgta
      121 tatgaggccg ttcagcaata taatcatgtt tatatgattt gcgataatag taattatttt
      181 ttatacagtc ttcttttttc aggggggcct gttaaatgca tatgtgctga taatataagt
      241 atccactatg gaaatcggtt atcaattgat tatacgtatc caccagaaca aattcttcga
      301 tctcttaata ataccgaacg agagattctt tacctctatt tcattaagcg gagaaaaata
      361 aaagaaattt caaagattac atgtttaagt gtaagtaaaa tttattatag aataagaatg
      421 ataaaaatta aatttggtgc tgaaacgaca cggaagcttc caattctttt taggtgttat
      481 ctgtaacatc aactgatttt attttcatga cgtaactatt atcagagtca attaatttta
      541 tcatcatagt gactaattat ttatagtcta tgaaattagt tgatatgtcg atgcgaaact
      601 ttttaccggg aagtattcat ctttgcgtta tttccgagtt tacagagtgg atatcccatg
      661 aaattatgtt gttacaaagc atgggggtga ctgtatgaat agtcaaaatg ctttaagtaa
      721 tatgaggctt gctgtgagag gggatatatc cggtaactct acattggttc ttaaaaagat
      781 tagattctac aattgtgcca ttatttatct tcgcaatgcc caattacttg ttaaaacgat
      841 agatgggggg gttattaata tcccacctga atcaatatgt tatattgaga aaaatacagt
      901 cattgatgta acacttaacg tgttaggtaa aggcatgcct tatgatattt ttcatataga
      961 taataatata ttaagctgta tctgcaaagt catggaacca ttgttactga atccccataa
     1021 aattggccct atgcgaagta gaatttttaa ttgcatggcg gataagacag atacagaaat
     1081 atttaaaatg ataacagagg caaatgtccc aaatcataga ctaatttata acattactta
     1141 tttgttatct aaagttgacg atattgaatc tttggtttgc tcattatctg tttcaacaga
     1201 ctctactttc acagaaaaat taaagttaat aatagaatct gatctgtcaa aatcatggcg
     1261 attagttgat ctggcaaatg ttttacatat gtctgaagtc tcaattcgga aaaaacttga
     1321 aaaagaaaat aataatttta acaatttgat tttggatatt agaatgcaac atgcagcaag
     1381 attgataact accactgaaa aacatattaa tagtatatct gatgaggttg ggtatgttag
     1441 tacatcatat tttatccgta attttaaaag ttattttggt ataacaccaa aacagttttc
     1501 attgaaagtg aaaaggaaat cataatatat attgcatata taatataggc agaagcaatt
     1561 cttactgatt t
//
LOCUS       DAOFSV010000321         1552 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.325, whole genome shotgun sequence.
ACCESSION   DAOFSV010000321 DAOFSV010000000
VERSION     DAOFSV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.325"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..957
                     /locus_tag="QBH27_004861"
     CDS             <1..957
                     /locus_tag="QBH27_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDQ7017050.1"
                     /translation="VNSCLIIHDNNKLGDLIVLSSIYRELYSKGVKITILTNSKGGAF
                     LSNNKNIFEFCIKESTGFLKMLTLCKHLRDLQFDIVLDPFETMPSFKHSLILSSLKDS
                     YILGFDQWYKRYYSFYHPHDECLKEHMSTRATEILKHIYGEGEFSTDYDLHLPVDVED
                     KIKEFIGDTRIVIINPLGAKKICRLTFEQIKVIYQEMKLHFENYRVIFTGLPQDLLTI
                     PIPEIETLPFNEFIYTVALTKYSDFVISVDTALVHIAAAYHKPTLAFYPNSRTPEYPS
                     HLIWSPNNHKSIQIVSPTFTVKDIDTETLTDSVKRLSCIDKK"
     gene            complement(983..>1552)
                     /gene="waaL"
                     /locus_tag="QBH27_004862"
                     /gene_synonym="rfaL"
     CDS             complement(983..>1552)
                     /gene="waaL"
                     /locus_tag="QBH27_004862"
                     /gene_synonym="rfaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HDQ7017051.1"
                     /translation="YNKSPKKFTSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSY
                     TNANSVTSLGARLAMYEIGLNIFIKSPFSFRSAESRAENMNLLVAEHNRLRGALEFSN
                     VHLHNEIIEAGSLKGLMGIISILFLYFSLFYTAYKRRALGLLILTLGIVGIGLSDVII
                     WARSIPIIVISAIVLLLVINNRNNTINQE"
BASE COUNT          523 a          262 c          254 g          513 t
ORIGIN      
        1 gttaattcat gtcttattat tcatgataat aataagctcg gtgatctaat tgtattaagt
       61 tcgatttatc gtgaacttta tagtaaaggg gttaaaataa ccattcttac aaatagtaag
      121 ggtggggcgt ttctctctaa caataaaaat atatttgagt tctgtattaa agaatcaacc
      181 ggtttcctta aaatgcttac tctatgtaag catctgcggg atttgcagtt cgatattgta
      241 ttagacccct ttgaaaccat gccctcattt aagcatagtt taatattatc aagtttaaaa
      301 gattcttaca ttctgggttt tgatcaatgg tataaacgtt actattcatt ttaccatccc
      361 cacgatgaat gtttaaaaga acatatgagt acacgagcaa cagaaatact gaagcatatt
      421 tatggtgagg gtgaattctc caccgactac gacttgcatt taccagtaga tgttgaagat
      481 aaaataaaag aatttattgg agatacaaga atagtcatta ttaacccatt aggtgcaaaa
      541 aaaatatgcc gacttacgtt tgagcaaata aaagttatat atcaagaaat gaaactacac
      601 tttgaaaatt atcgggtgat atttaccggg ttaccgcaag atttattgac aataccaata
      661 cctgaaatcg aaacactacc atttaatgag tttatttata ccgttgcgtt gaccaagtat
      721 agtgattttg tcatttctgt tgatacagct cttgttcata tcgctgcggc ttatcataaa
      781 ccaacgcttg cattttatcc taattcacgt actccggaat atccctcaca tttaatttgg
      841 tccccaaata atcataaatc aatacaaata gtatccccaa catttacggt gaaagatatc
      901 gatactgaaa ctttaactga ttcagtaaag agattaagtt gtatagataa gaagtgagtt
      961 ttaactcact tcttaaactt gtttattctt gattaatagt attgttacga ttattaataa
     1021 cgagtaagag gactatagcg gatataacga taattggaat gctgcgtgcc catatgatca
     1081 catcactgag tccaatcccc acaatgccaa gcgttaatat caacaaaccc aaagctcgtc
     1141 ttttatatgc tgtataaaac agtgaaaaat agaggaaaag gatggaaata attcccatca
     1201 gacctttcag tgaccctgct tcaattatct cattatgtag atgtacgtta gaaaactcca
     1261 atgcccctct tagcctgttg tgttctgcaa ctaacaaatt catattttca gcgcgtgact
     1321 ctgctgatct aaatgaaaaa ggtgacttta tgaatatatt taaaccaatt tcgtacattg
     1381 ccagtcttgc acctagggaa gtcacgctat tagcattggt ataactgttt aaatcattta
     1441 atgcttcatt atagcgattc tgtattggtt tattaaatat aattactatg ctagctaata
     1501 tagcaattag tagaacaata gaggaagtga atttcttggg tgatttatta ta
//
LOCUS       DAOFSV010000322         1549 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.326, whole genome shotgun sequence.
ACCESSION   DAOFSV010000322 DAOFSV010000000
VERSION     DAOFSV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.326"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            351..476
                     /locus_tag="QBH27_004863"
     CDS             351..476
                     /locus_tag="QBH27_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ7017052.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="QBH27_004864"
     CDS             complement(921..1397)
                     /locus_tag="QBH27_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ7017053.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          357 a          405 c          385 g          402 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggtttaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atcctggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAOFSV010000323         1542 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.327, whole genome shotgun sequence.
ACCESSION   DAOFSV010000323 DAOFSV010000000
VERSION     DAOFSV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.327"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..1426)
                     /gene="yfcC"
                     /locus_tag="QBH27_004865"
     CDS             complement(<1..1426)
                     /gene="yfcC"
                     /locus_tag="QBH27_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDQ7017054.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDF"
BASE COUNT          463 a          405 c          372 g          302 t
ORIGIN      
        1 ggaagtccac ccggcaaaca ccgagcgtcg ccattaacga agctgaggtt gggtaaatga
       61 tgtggctgaa gccatcacca aactggaacg ccagcacggt aacctgacgg ttaacaccga
      121 ccagatcgcc aagcggtgcc agtaacggca tggttaacgc cgcctgacca gaaccggacg
      181 tcacgaagaa attaaatacc gcctggaaga gcaacataaa ccaggccgcg actgcgttat
      241 ccagaccgct aatggcattg gcaatgctgt tgaggatggt atttaacacg ctggcatcac
      301 ccgcttcacc attaccgacc agcagcaaaa tccctttggc gaaacccacc agcagggcag
      361 gggcgatcat cattcgcgcc ccttcggtaa aggatgaagc catggtatta accgtcatgc
      421 cgttaaggcg gaaaacaacg ccgatgatgc caatcaccag acccatggtg aagaactggc
      481 tggcaatttc aggaataaac caggcattaa cgatcacgcc ccaaataacc cagaccatta
      541 cggcggtcag gacaatcaat accagccagt caccaaaggt aaacggacgt tgttcaacat
      601 cagcctgctt ttcacgaaag aagcggtcgg actcatgcac gcgtgacaga agaggattct
      661 ttttcactcg tgaggcgtac accatggtaa agatcaggcc aatcagagtg gcgataaccc
      721 acaccacgat gcgcaacccg gagccagaaa gcaccggaac gccggcaata ccctgagcaa
      781 caaccacaca aaacgggttc atccacgaac tggcaaaacc gatttgcgtg gcaatatagg
      841 tcacaaggac ggtggtaata ctgtcatagc ccagccggac cattagcggt gcgatgataa
      901 tggcaaaggc gacggcctct tctcccatac caaatattgc gccgccaagt gaaaacagaa
      961 taaacagcgc aggaataaag agaatttcat ttccgcgggt atggcgaata agcgccagga
     1021 taccgttatc aatggttcct gtacgcatca caatgccaaa cgcgccgcca atcaccagca
     1081 taaacatgat gatgccaacg gctgtcccgt atttcgatcc tgaggttaac ccttcaaacg
     1141 ggaagttcat cagacccggg cgttcatcgc ccgtcgtgaa cagctgtacg cggtgatact
     1201 caggttcgcc tgcttcgtta gtcagaatgc gaaatgagtg tggatctacg actttgcgtg
     1261 tttttgtttg accatcaacc tgatactgca cttcctgact gtcaaacatg cccaccggaa
     1321 ctacccaggt ggcaaggctg gttaaaatgg caacaaaaaa gataatcacc aacgtatcgg
     1381 gcattgccca tcttcttgtt ggtttagatt cagtgattgc ggacatagcg caaatattcc
     1441 cttgcacaaa acaaagtggt aagtatgcaa agtgagatgg cgcaattcat tgatgcagcg
     1501 cagttaagca agataatcag aaaggattaa tgcaaattaa ga
//
LOCUS       DAOFSV010000324         1541 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.328, whole genome shotgun sequence.
ACCESSION   DAOFSV010000324 DAOFSV010000000
VERSION     DAOFSV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.328"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            515..817
                     /locus_tag="QBH27_004866"
     CDS             515..817
                     /locus_tag="QBH27_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDQ7017055.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            864..1286
                     /locus_tag="QBH27_004867"
     CDS             864..1286
                     /locus_tag="QBH27_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDQ7017056.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            1283..1474
                     /locus_tag="QBH27_004868"
     CDS             1283..1474
                     /locus_tag="QBH27_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017057.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT          310 a          412 c          481 g          338 t
ORIGIN      
        1 ctttttcctg ccggtccccg gacagagcac aaaggaagtc gccgccggct ccgctttacc
       61 cggccatgca gggcatggcc ttgtggtttt tcagttatgt ggcctcagcg tcgtgtgcgg
      121 gctgtgccgt gcctccatct tagccgggct ggcgttgatg caagggtacg cttcgccgct
      181 gcggtcaccc ggtccctcct tcccgtctgc cgtgattttc cggtcgttca cccgctcaga
      241 tttcgcggtc tcaccctgca actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg
      301 cgcccctgca tccccgcctg ttcgcccggc ttttatggag gcacggcacc gcccggtgtc
      361 gcggaatatg tgaacaacgg aggaaaacat catgcaatat gcgaaacctg tcactctgaa
      421 tgttgaagag tgcgaccgtc tctcctttct gccgtatctg tttggcaacg attttctgta
      481 tgccgaggcg tatgtgtacg cgctggcgca aaaaatgatg ccggaatatc agggaggctt
      541 ctggcacttc atccgcctgc cggacggtgg cggttacatg atgccggatg gcgaccgttt
      601 ccacatggtg aacggtgcaa actggtttga ccgtaccgtg agtgctgatg ccgcaggcat
      661 catcctgacc tcccttgtga ttaaccgcca gttgtggctg taccacgaca gcggggatgc
      721 aggactgacc cagctttacc ggatgcgcga tgcgcagttg tggcgtcaca tcgaatttca
      781 ccccgaatgc aacgcgattt acgcggctct ggactgatta acggacgggg cggcaatgcc
      841 gcccctgtaa aggagacgag aacatgtact gtactgttaa agaaattatc cgcgaagtgc
      901 tggatacaga cgtgccggac agtgaatgcg tttttgccgt agtgctgacc cgtggggatg
      961 tgcgtcacat agcccaggac tggagtctga cagacgatga gctggaaacc gtcatgcagc
     1021 ggctggacga tgcctttgaa tatggtgcgg atgtcagcgt tgttcacggc gttgttcgtg
     1081 aactgatgga agaaaagcgc gccagccgtc aggtgacagt cccggcggtg atgctggaaa
     1141 aagtgatggc gctggcaggc agtgaaatga agcgcctgta tgccgtcggg agtgagaacg
     1201 ggggcgacgg tgacgcattc gtcagggagg aacgcgaagc aatggacgtt gtgttacagg
     1261 cgctggacgg ggagacgatg tcatgaatat cagcacagaa acccgcgaaa ttctgcgtaa
     1321 ttacagggcc gtgattaatg cgcggcgtcg tgaaatgggg cagaaaccgc tcaccaccgc
     1381 gcagattgtt gatgaaatct gcgattttgt ggcgaatcag caggcggttt tcctgggcgg
     1441 tcactacatc ttacagggta gcagaaacag gtgatacagg ggcaggcggt ggaggccgcc
     1501 tgtcagtccg cgcgaaaacc cctggcaggc tgacgcctgc c
//
LOCUS       DAOFSV010000325         1535 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.329, whole genome shotgun sequence.
ACCESSION   DAOFSV010000325 DAOFSV010000000
VERSION     DAOFSV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.329"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            96..821
                     /gene="pyrH"
                     /locus_tag="QBH27_004869"
     CDS             96..821
                     /gene="pyrH"
                     /locus_tag="QBH27_004869"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDQ7017058.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            971..1528
                     /gene="frr"
                     /locus_tag="QBH27_004870"
     CDS             971..1528
                     /gene="frr"
                     /locus_tag="QBH27_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDQ7017059.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
BASE COUNT          406 a          349 c          426 g          354 t
ORIGIN      
        1 aattcagcta acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa
       61 ttcatttcaa tcgttgacag tctcaggaaa gaaacatggc taccaatgca aaacccgtct
      121 ataaacgcat tctgcttaag ttgagtggcg aagctctgca gggcactgaa ggcttcggta
      181 ttgatgcaag catactggat cgcatggctc aggaaatcaa agaactggtt gaactgggta
      241 ttcaggttgg tgtggtgatt ggtgggggta acctgttccg tggcgctggt ctggcgaaag
      301 cgggtatgaa ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta atgaacggcc
      361 tggcaatgcg tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg tccgctattc
      421 cattgaatgg cgtgtgcgac agctacagct gggcagaagc tatcagcctg ttgcgcaaca
      481 accgtgtggt gatcctctcc gccggtacag gtaacccgtt ctttaccacc gactcagcag
      541 cttgcctgcg tggtatcgaa attgaagccg atgtggtgct gaaagcaacc aaagttgacg
      601 gcgtgtttac tgctgatccg gcgaaagatc caaccgcaac catgtacgag caactgactt
      661 acagcgaagt gctggaaaaa gagctgaaag tcatggacct ggcggccttc acgcttgctc
      721 gtgaccataa attaccgatt cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg
      781 tggtaatggg tgaaaaagaa gggactttaa tcacggaata attcccgtga tggataaata
      841 agggtaagat tccgcgtaag tatcgcgggg gatcttaccc gtaatatgtt taatcagggc
      901 tatacttagc acacttccac tgtgtgtgac tgtctggtct gactgagaca agttttcaag
      961 gattcgtaac gtgattagcg atatcagaaa agatgctgaa gtacgcatgg ataaatgcgt
     1021 agaagcgttc aaaacccaaa tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct
     1081 ggatggcatt gtcgtggaat attacggcac gccgacgccg ctgcgtcagc tggcaagcgt
     1141 aacggtagaa gattcccgta ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc
     1201 cgttgaaaaa gcgattatgg cgtccgatct tggcctgaac ccgaactctg cgggtagcga
     1261 catccgtgtt ccgctgccgc cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt
     1321 tcgtggtgaa gcagaacaag cgcgtgttgc agtacgtaac gtgcgtcgtg acgcgaacga
     1381 caaagtgaaa gcactgttga aagataaaga gatcagcgaa gacgacgatc gccgttctca
     1441 ggacgatgta cagaaactga ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga
     1501 caaagaagca gaactgatgc agttctgatt tcttg
//
LOCUS       DAOFSV010000326         1511 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.330, whole genome shotgun sequence.
ACCESSION   DAOFSV010000326 DAOFSV010000000
VERSION     DAOFSV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.330"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            247..1122
                     /locus_tag="QBH27_004871"
     CDS             247..1122
                     /locus_tag="QBH27_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HDQ7017060.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1157..>1511
                     /locus_tag="QBH27_004872"
     CDS             1157..>1511
                     /locus_tag="QBH27_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDQ7017061.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSV"
BASE COUNT          373 a          374 c          431 g          333 t
ORIGIN      
        1 gaatatcatc aataaaattg agtgttgctc tgtggataac ttgcagagtt tattaagtat
       61 cattgcagca aagatgaaat caatgattta tcaaaaatga ttgaaaggtg gttgtaaata
      121 atgttacaat gtgtgagaag cagtctaaat tcttcgtgaa atagtgattt ttgaagctaa
      181 taaaaaacac acgtggaatt tagggaatac tgatgtaaca cggattgacc gtattgggag
      241 tttgagatgg tgacaaagag agtgcaacgg atgatgttcg cggcggcggc gtgcattccg
      301 ctgctgctgg gcagcgcgcc gctttatgcg cagacgagtg cggtgcagca aaagctggcg
      361 gcgctggaga aaagcagcgg agggcggctg ggcgtcgcgc tcatcgatac cgcagataat
      421 acgcaggtgc tttatcgcgg tgatgaacgc tttccaatgt gcagtaccag taaagttatg
      481 gcggccgcgg cggtgcttaa gcagagtgaa acgcaaaagc agctgcttaa tcagcctgtc
      541 gagatcaagc ctgccgatct ggttaactac aatccgattg ccgaaaaaca cgtcaacggc
      601 acaatgacgc tggcagaact gagcgcggcc gcgttgcagt acagcgacaa taccgccatg
      661 aacaaattga ttgcccagct cggtggcccg ggaggcgtga cggcttttgc ccgcgcgatc
      721 ggcgatgaga cgtttcgtct ggatcgcact gaacctacgc tgaataccgc cattcccggc
      781 gacccgagag acaccaccac gccgcgggcg atggcgcaga cgttgcgtca gcttacgctg
      841 ggtcatgcgc tgggcgaaac ccagcgggcg cagttggtga cgtggctcaa aggcaatacg
      901 accggcgcag ccagcattcg ggccggctta ccgacgtcgt ggactgtggg tgataagacc
      961 ggcagcggcg gctacggcac caccaatgat attgcggtga tctggccgca gggtcgtgcg
     1021 ccgctggttc tggtgaccta ttttacccag ccgcaacaga acgcagagag ccgccgcgat
     1081 gtgctggctt cagcggcgag aatcatcgcc gaagggctgt aactggtttt gttgaataaa
     1141 tcgaactttt gctgagttga aggatcagat cacgtatctt cccgacaacg cagaccgttc
     1201 cgtggcaaag caaaagttca aaatcaccaa ctggcccacc tacaataaag ccctcatcaa
     1261 ccgtggctcc ataactttct ggctggatga tgaagctatt caggcctggt atgagtcagc
     1321 aacaccttct tcacgaggca gacctcagcg ctattctgac cttgccatca cgactgtgct
     1381 ggtcattaaa cgcgtattca ggctgaccct gcgcgctgcg cagggcttta ttgattccat
     1441 tttttctctg atgaacgttc cgctacgctg cccggattac agctgtgtca gcaggcgggc
     1501 aaagtcggtt a
//
LOCUS       DAOFSV010000327         1460 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.331, whole genome shotgun sequence.
ACCESSION   DAOFSV010000327 DAOFSV010000000
VERSION     DAOFSV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.331"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            55..288
                     /gene="papI"
                     /locus_tag="QBH27_004873"
     CDS             55..288
                     /gene="papI"
                     /locus_tag="QBH27_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HDQ7017062.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
     gene            complement(939..>1460)
                     /locus_tag="QBH27_004874"
     CDS             complement(939..>1460)
                     /locus_tag="QBH27_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7017063.1"
                     /translation="GLAGVLRGKKVRTTVSRKTVAAGDRVNRQFVAERPDQLWVADFT
                     YVSTWQGFVYVAFIIDVFAGYIVGWWVSSSMETTFVLDALEQALWARRPSGTIHHSDK
                     GSQYVSLAYMERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVE
                     LATLTWVDWYNNR"
BASE COUNT          381 a          369 c          327 g          383 t
ORIGIN      
        1 gttgataaca gagagcatga tttttattat tcgaagaaac tttgttacag tgagatgagt
       61 gaatatatga agaatgaaat actggaattt ctgaacaggc ataatggagg gaaaaccgca
      121 gaaattgcgg aggcgctggc ggtaacggat tatcaggcac gctattacct gttattactg
      181 gaaaaagaag gtgtggttca gcgctctcca ttaaggcgag gcatggcaac atactggctt
      241 ctgaaaggag agaagcaggc ggggcagaac tgttcttcca caacttaata actcaatctg
      301 tgggcatgct tcactgaaac agataaaagt tacatcagag tgcagattca gttttgcagc
      361 attcatcaca aaatagaaat ttagtatttt atgtcagaat acttcacatg ataataaaaa
      421 cctgtctgga tttactgcat ggccagacat tacaaaatac aaacgccata aacttcattc
      481 agccttcttg ctgtccggta acggaggctg ctttttcgtt acacctttcc ccggcagcat
      541 cagtagcagt aatttataga ttctacgaac tgagactgtc tgtccgggga gaacgtacac
      601 agcccgacac aatgtaccag tcaataatat ccggtttctg attccctgcc tgacagacca
      661 ctctctggca actttgtatt cagttcatct actactggag acagatttat aaaatccaac
      721 tccacgatct ttgaccgtcc gcatccccta ctctctgtgc tgcggtcatc ggttaattcc
      781 agcgtccgtc cgttacgatc agatattact gaaccgcccc gggtttcctg gagagtgttt
      841 tatctgtgaa ctcaggctgc cagatcattg tttccgatgg aagcataata agctttttct
      901 gcttctgccg gaggagtatg gcccagcctt cccagcaatc atcgattgtt ataccagtcc
      961 acccacgtga gtgtggccag ttccacttct gcacggtttt tccagctctt acggtgtatt
     1021 acctccgctt tgtaaagacc attgatgctc tccgccatcg cgttgtcata cgagtcgcct
     1081 gtactccctg ttgatgccag cagttttgct tcttttaatc gctccatata ggccagtgac
     1141 acatactgag agcctttatc gctgtgatgg atggtgccgg acggacggcg ggcccacaac
     1201 gcctgctcca gcgcatccag cacgaatgtc gtttccatag acgatgagac ccaccacccc
     1261 acgatgtatc cggcaaacac atcaatgatg aacgccacat agacgaagcc ctgccatgtg
     1321 ctgacgtaag taaaatcagc cacccacagc tggtcaggac gttctgccac gaactgacgg
     1381 tttacgcggt cacctgcagc aacggttttc cggctgacgg tagtgcggac ctttttaccc
     1441 cggagaacac cggcaagtcc
//
LOCUS       DAOFSV010000328         1457 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.332, whole genome shotgun sequence.
ACCESSION   DAOFSV010000328 DAOFSV010000000
VERSION     DAOFSV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.332"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            228..515
                     /locus_tag="QBH27_004875"
     CDS             228..515
                     /locus_tag="QBH27_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017064.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            634..1455
                     /locus_tag="QBH27_004876"
     CDS             634..1455
                     /locus_tag="QBH27_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDQ7017065.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          421 a          301 c          378 g          357 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaacaga tgatgggggc tgaaaaccag agagaggaga aagcaggatg agtacccgaa
      241 atatccacgt taacaccgcg tcgtacacgc ttcttgttgc cgggaagaaa aaaaacaccg
      301 gggaagagtg ggacgtcctg gaattcagca gcctgactga gctgaaaaaa tatcgcaaaa
      361 gccacccgga aaagatggcc ttcagttata gctacgcgct cagtcagggt gtggataagc
      421 agttccgcca tatcaacatt gcggaagccg atcacttcaa acagttcctg cgtcagatta
      481 agcgtgccgg cctcgatatc cgggcgatct gctgaccgga tgtgatgaat acccctcctg
      541 attgttgcaa tcttgttaat ggagatcggg ggggatgata ttaagcggaa aaatttttta
      601 ttaaatttat tctttcataa aggagtagct gttatgcgat tagcttcccg ttttggtcgg
      661 tataattcca tccgccgtga acgtccttta acggatgatg aattaatgca gttcgtgcct
      721 tcggtatttt ccggtgataa acatgagtcc cggagtgaac gttatacgta tattccaaca
      781 atcaatatca tcaataagtt acgtgatgaa ggtttccagc cattctttgc ctgtcagagt
      841 cgggttcgtg atttgggacg tcgcgaatac agtaaacata tgttacgtct tcgcagggaa
      901 gggcatatta acggacagga agttcctgaa attatcctgc ttaattcaca tgatggttca
      961 tccagttatc agatgatccc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc
     1021 ggaaataatt ttggcgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc
     1081 gagggagcgt atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg
     1141 aaagaaattc atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc
     1201 aggtatgaag atgaaaataa aacgccagtg acacctgaac agataattac tccccgtcgt
     1261 cgggaagaca aacagaacga tctctggaca acctggcagc gggttcagga gaatatgata
     1321 aaaggtggat tatcggggcg aagtgcctcc gggaaaaata ccaggacaag agccattaca
     1381 ggtattgatg gtgatataag aatcaacaag gcgttatggg tgattgccga acagttcaga
     1441 aagtggaagt catgata
//
LOCUS       DAOFSV010000329         1407 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.334, whole genome shotgun sequence.
ACCESSION   DAOFSV010000329 DAOFSV010000000
VERSION     DAOFSV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.334"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(34..507)
                     /locus_tag="QBH27_004877"
     CDS             complement(34..507)
                     /locus_tag="QBH27_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HDQ7017066.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            665..>1407
                     /gene="intI1"
                     /locus_tag="QBH27_004878"
     CDS             665..>1407
                     /gene="intI1"
                     /locus_tag="QBH27_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDQ7017067.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWLWFWVFAQHTHSTDPRSGVVR"
BASE COUNT          323 a          353 c          365 g          366 t
ORIGIN      
        1 aaggagcgaa gcgactggtg ctgcaacgca ttgttagcct tttttccaaa tctggtatgt
       61 ataatttata ttagacataa aaaactgttc aaaaaccaaa ttgaaattct caggcattat
      121 agggaatttg atatcacctt cgacttcaac gtgaacagta gacaaatgaa ttatatctgc
      181 tttttcaata aggctattat agatttgacc cccgccagag acatatacat gatctgtaac
      241 ttttgatagc tctttcaaag cattttctat tgaaggaaaa actaggacgt tttcatttga
      301 gcttgaaatt ccgttctttg acactactgc atatttgcga tttggaagaa cacccataga
      361 gtcaaatgtt tttcttccga caaggagcca ttgattatat gtgagcgctt taaagagtag
      421 ttgctcacct tttactgacc acgggatatc aggaccacta ccgattacgc cattttctga
      481 cactgcagaa atcaatgata ttttcaattt aactccctta atggctaact ttgttttagg
      541 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
      601 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
      661 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
      721 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcca
      781 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
      841 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
      901 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
      961 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
     1021 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagct
     1081 tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca aggatctgga
     1141 tttcgatcac ggcacgatca tcgtgcggga gggcaagggc tccaaggatc gggccttgat
     1201 gttacccgag agcttggcac ccagcctgcg cgagcagctg tcgcgtgcac gggcatggtg
     1261 gctgaaggac caggccgagg gccgcagcgg cgttgcgctt cccgacgccc ttgagcggaa
     1321 gtatccgcgc gccgggcatt cctggctgtg gttctgggtt tttgcgcagc acacgcattc
     1381 gaccgatcca cggagcggtg tcgtgcg
//
LOCUS       DAOFSV010000330         1397 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.335, whole genome shotgun sequence.
ACCESSION   DAOFSV010000330 DAOFSV010000000
VERSION     DAOFSV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.335"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            1..259
                     /locus_tag="QBH27_004879"
                     /pseudo
     CDS             1..259
                     /locus_tag="QBH27_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            345..1367
                     /gene="istA"
                     /locus_tag="QBH27_004880"
     CDS             345..1367
                     /gene="istA"
                     /locus_tag="QBH27_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HDQ7017068.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
BASE COUNT          332 a          374 c          377 g          314 t
ORIGIN      
        1 gctgtctccg ctggaagagg cgcttcatgg ctatgtgctg actgacggta agctccatgc
       61 tgatgacacg cctgtcccgg tgctgttgcc aggcaataag aaaacgaaga ccgggcggtt
      121 atggacctac gttcgtgacg accgtaacgc cgggtcaacg ctggcgccgg cggtgtggtt
      181 cgcttacagc ccggacagaa aaggcatcca tccgcagacc catcttgcgg ggttcagtgg
      241 tgtactgcag gcggatgctg tcacgaacgg tgcaatagtg ctccacaccc aacgcctgaa
      301 atcagatcca gggggtaatc tgctctcctg attcaggaga gtttatggtc acttttgaga
      361 cagttatgga aattaaaatc ctgcacaagc agggaatgag tagccgggcg attgccagag
      421 aactggggat ctcccgcaat accgttaaac gttatttgca ggcaaaatct gagccgccaa
      481 aatatacgcc gcgacctgct gttgcttcac tcctggatga ataccgggat tatattcgtc
      541 aacgcatcgc cgatgctcat ccttacaaaa tcccggcaac ggtaatcgct cgcgagatca
      601 gagaccaggg atatcgtggc ggaatgacca ttctcagggg attcattcgt tctctctcgg
      661 ttcctcagga gcaggagcct gccgttcggt tcgaaactga acccggacga cagatgcagg
      721 ttgactgggg cactatgcgt aatggccgct caccgcttca cgtgttcgtt gctgttctcg
      781 gatacagccg aatgttgtac atcgaattca ctgacaatat gcgttatgac acgctggaaa
      841 cctgccatcg taatgcgttc cgcttctttg gtggtgtgcc gcgcgaagtg ttgtatgaca
      901 atatgaaaac tgtggttctg caacgtgacg catatcagac cggtcagcac cggttccatc
      961 cttcgctgtg gcagttcggc aaggagatgg gcttctctcc ccgactgtgt cgccccttca
     1021 gggcacagac taaaggtaag gtggaacgga tggtgcagta cacccgtaac agtttttaca
     1081 tcccactaat gactcgcctg cgcccgatgg ggatcactgt cgatgttgaa acagccaacc
     1141 gccacggtct gcgctggctg cacgatgtcg ctaaccaacg aaagcatgaa acaatccagg
     1201 cacgtccctg cgatcgctgg ctcgaagagc agcagtccat gctggcactg cctccggaga
     1261 aaaaagagta tgacgtgcat cttgatgaaa atctggtgaa cttcgacaaa caccccctgc
     1321 atcatccact ctccatctac gactcattct gcagaggagt ggcgtgatga tggaactgca
     1381 acatcaacga ctgatgg
//
LOCUS       DAOFSV010000331         1391 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.336, whole genome shotgun sequence.
ACCESSION   DAOFSV010000331 DAOFSV010000000
VERSION     DAOFSV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.336"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..183
                     /locus_tag="QBH27_004881"
     CDS             <1..183
                     /locus_tag="QBH27_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDQ7017069.1"
                     /translation="DGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVK
                     TKVQGTVVMFSASGKK"
     gene            complement(238..336)
                     /locus_tag="QBH27_004882"
                     /pseudo
     CDS             complement(238..336)
                     /locus_tag="QBH27_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(382..564)
                     /locus_tag="QBH27_004883"
     CDS             complement(382..564)
                     /locus_tag="QBH27_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ7017070.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            690..>1391
                     /locus_tag="QBH27_004884"
     CDS             690..>1391
                     /locus_tag="QBH27_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ7017071.1"
                     /translation="MTGELKIGTVNALRIFNDAFGLIFRRSEDFLHFIPTAEGQGENG
                     DIGPLRPFAINLRTGAISVSHGAKIDGGLALGTDNALGGNSITLGDNDTGIKQGGDGV
                     LLFYSNGQLAFGLQPASADFYKRVAYIHQGIIPDGSGAFADQLNNATAPFVQTQFAWN
                     PTPGGLYVPIVKGLSIRNGQGYPGAVSFGYLLTEQYGFPVPCIHMRGDGGNDALWQFN
                     PNDKSFISPGALIAGG"
BASE COUNT          366 a          324 c          319 g          382 t
ORIGIN      
        1 gatggagttg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc
       61 atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc
      121 gagaatgtta aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa
      181 tagcatccaa ccgcagcacg ttcttgctta agacgtgctg cggcataatc ccaatgatta
      241 ctccctgaca gggttcgtag gccactcaat atcaggtgca gttgatgtat caacacggtt
      301 cagtaacacc cgatactttt tctaggcttc caccaccagc acgacaagat gccgcataca
      361 gtgaaccagt cagtccagtt ttcagacaac cagtgcgtca cctttttgaa ggcgctttaa
      421 agcacgtttt aatccaggtc ggcctgtcct tgttccgctt aatttatctt caaatatttg
      481 ttcatatcct gcacaaacaa gagcgtttcg ttgcagatct gtattctggt catttgttga
      541 tacccttaca tagccaatca gcacgctgaa tctcccgtcc aaaagcacaa atcatgccat
      601 gcaggccaga aaccgccatt atctaaaacc tcggtttaca ggaaacggta aacaaggctg
      661 gtaacgccgt tcagcgttcc ggcgataaaa tgaccggaga actgaaaatt ggcacggtga
      721 atgcgctgcg aattttcaat gatgccttcg gtcttatttt ccgtcgttca gaagattttc
      781 ttcatttcat tccgacggct gaaggacaag gcgaaaacgg tgatatcggc ccattaaggc
      841 cattcgctat aaatctgaga acaggtgcta tatctgtcag ccacggggcc aaaattgatg
      901 gtgggctggc gcttggtaca gataacgcac tgggcggtaa ttccattact ctcggagata
      961 acgacactgg tattaaacag ggcggcgacg gtgtcctttt attctattca aatggacaac
     1021 tggcatttgg gcttcaaccc gcatctgctg atttttataa gcgggttgca tatattcatc
     1081 agggaataat tcctgatgga agtggcgcat ttgcagacca gttgaataat gccaccgcgc
     1141 cttttgttca gacgcagttt gcctggaatc ccactcctgg tggtctttac gtgccgatag
     1201 ttaagggctt gtccattcgc aatggacagg gctatcccgg cgcggtcagc tttgggtatt
     1261 tactgacaga acagtatgga tttccggttc catgtattca tatgcgtggc gatggcggta
     1321 atgatgcttt atggcagttt aacccgaacg ataaatcctt tatttcaccg ggtgctctta
     1381 ttgcgggtgg c
//
LOCUS       DAOFSV010000332         1352 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.337, whole genome shotgun sequence.
ACCESSION   DAOFSV010000332 DAOFSV010000000
VERSION     DAOFSV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.337"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..702
                     /gene="hipA"
                     /locus_tag="QBH27_004885"
     CDS             <1..702
                     /gene="hipA"
                     /locus_tag="QBH27_004885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDQ7017072.1"
                     /translation="LLAKELGLNVPDAEIIKAGRVRALAVERFDRRWNTERTVLLRLP
                     QEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATD
                     GHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAID
                     KIYPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETN
                     VLRLHDRLSREYGIK"
BASE COUNT          378 a          281 c          306 g          387 t
ORIGIN      
        1 ctgctggcga aagaactcgg gttgaatgtt ccggacgcag aaatcattaa agcgggaagg
       61 gtgcgcgcgt tagcggtcga acgttttgac aggcgttgga atactgagcg aacggtttta
      121 cttcgcttgc cacaggagga tatgtgccag acattcggtt taccttcatc ggtgaaatat
      181 gaatcagatg gaggccccgg tatcgcgcaa attatggctt ttttgatggg gtccagcgag
      241 gcgctgaaag atcgctatga ttttatgaag tttcaggtct tccagtggtt gattggcgca
      301 acggatggcc atgcaaaaaa cttctccgta tttattcagg ctggcggcag ttatcggctc
      361 acaccatttt acgacatcat ttcggcattt ccggtccttg gcggtacggg aatacacatc
      421 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
      481 aaaatttatc cgcgacattt tctggcgaca gcaaaggtgc tgaaattccc ggaagtgcag
      541 atgcatgaaa tcctgagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
      601 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
      661 aggttgcacg atcggttaag ccgagaatac ggtattaagt aagatatggg gttttggtcg
      721 ttgttaatga gcagaacaat catgaaccgc caacgactgg attcgccctt ggtttatatt
      781 ggtgcgcata atgtatatta tgttaaattg attatgaagg tcctttactt caccattcac
      841 atccacacac agataaatca atacgttgtg atttctgact tccctcccgt ttcggtagaa
      901 aaatcaacct ctgttcagtg atggcacacc atagaccatg agctggataa ttgcgttgtt
      961 tacagcagcc agcgctttga tggcactata tccctcacgc ttctgaatat aattcatagc
     1021 gccgtgtaat tatctttaat ctcaagttag ctaatctcag catatcgtgt tgatatgcaa
     1081 tgtgaaatac agagaacagt gactacagct gatggaggaa gctatttccg atattcgtca
     1141 acttccaaat atatcgttta aaacaggtat cagatatgaa agcactacta attactgcat
     1201 tattcatggg attcagttag tttcaccagg caaacattaa ccatagctaa tgtttatagc
     1261 catataaact attagtacct cttgaccttt cctgacactt cccttgttcg caataatatt
     1321 ctgtttagtc gctgtttata aataacatgc at
//
LOCUS       DAOFSV010000333         1340 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.338, whole genome shotgun sequence.
ACCESSION   DAOFSV010000333 DAOFSV010000000
VERSION     DAOFSV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.338"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..144
                     /gene="tuf"
                     /locus_tag="QBH27_004886"
     CDS             <1..144
                     /gene="tuf"
                     /locus_tag="QBH27_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ7017073.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="QBH27_004887"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="QBH27_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDQ7017074.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="QBH27_004888"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="QBH27_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDQ7017075.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
BASE COUNT          301 a          305 c          386 g          348 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca
//
LOCUS       DAOFSV010000334         1322 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.339, whole genome shotgun sequence.
ACCESSION   DAOFSV010000334 DAOFSV010000000
VERSION     DAOFSV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.339"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            321..872
                     /gene="papX"
                     /locus_tag="QBH27_004889"
     CDS             321..872
                     /gene="papX"
                     /locus_tag="QBH27_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HDQ7017076.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT          439 a          248 c          274 g          361 t
ORIGIN      
        1 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
       61 tacctacaac gtttatcgac ctcacacgaa caacgatggt ttaccgcctt gcttacatga
      121 agaaaagtgg aaacaggtta ttccggtgtc ctgattttgt gaaccactac aataatcctt
      181 tttcacagac ttgtcagcag ccagcattta tgttctttta cctgcgggaa tttatccgtc
      241 tgcctgtcgg gactactctg tcatacagaa atcaggtagg aataaattgt agtggaaagt
      301 cgatgtttac cggatgactg atgcgcgctt gtacacagac agtgtgtttc agtaatatgg
      361 agaataatga aatgaataac acagacacat tagaaaaaat aatcagacac caaaaaaaca
      421 aagaccccgc atatcccttt cgggaacatt tgttgatgca gctctgtatt cgcacaaata
      481 aaagaatgca ggataatata tctgaatttc tgggggtgta tggaataaat cactcagcat
      541 atatggttct caccacatta ttcgcagcgg agaaccattg tctgtcacct tcagagataa
      601 gccagaaact tcagtttacc agaactaata ttacccgcat tacagatttt ttagaaaaag
      661 ccggatatgt aaaaaggacg gatagcaggg aggatcgccg tgctaaaaaa atcagtctga
      721 catctgaagg tatgtttttt attcagaggc tcactcttgc acaaagcatg tatctgaaag
      781 aaatctggga ttatctgacc catgatgaac aggaactgtt tgaagtcatt aataaaaagt
      841 tactggcaca tttttctgat gccagctcat aaagtgcgaa atatctgagg atgctggata
      901 gcttcaggca agagaataat gattcttgca gatgtgtttt tccggataca aaaacaaaag
      961 taaaaaattg cagcgccagg ccacctttca aagcagggag gcctgtaccg cgtcgaaaat
     1021 ttcagccagt taatgtaatt gtctgaacca ggcacttcgc ccgggcagga gaaggagcgt
     1081 ggttgcggtc atcctgtata ctgaagtcgt tatgaaggaa taatacaaat aattgcccgt
     1141 tttctctgta cgatagaggc aggtgtgtat gattgcatta ctatgttttt gtgatataaa
     1201 aaactaaatg gtgagatatg cattttaaaa tataacaaaa atcaccatga aataagaata
     1261 attatccacg gagaacataa actgcagact gttacaatgc atgctgttca tcttgacaat
     1321 gt
//
LOCUS       DAOFSV010000335         1256 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.340, whole genome shotgun sequence.
ACCESSION   DAOFSV010000335 DAOFSV010000000
VERSION     DAOFSV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.340"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..1256
                     /locus_tag="QBH27_004890"
     rRNA            <1..1256
                     /locus_tag="QBH27_004890"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          313 a          292 c          395 g          256 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      601 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      661 agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac atccacggaa
      721 gttttcagag atgagaatgt gccttcggga accgtgagac aggtgctgca tggctgtcgt
      781 cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct tatcctttgt
      841 tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg aggaaggtgg
      901 ggatgacgtc aagtcatcat ggcccttacg accagggcta cacacgtgct acaatggcgc
      961 atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt cgtagtccgg
     1021 attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgtg gatcagaatg
     1081 ccacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtgggtt
     1141 gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg attcatgact
     1201 ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac ctcctt
//
LOCUS       DAOFSV010000336         1246 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.342, whole genome shotgun sequence.
ACCESSION   DAOFSV010000336 DAOFSV010000000
VERSION     DAOFSV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.342"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..207
                     /gene="cbtA"
                     /locus_tag="QBH27_004891"
     CDS             <1..207
                     /gene="cbtA"
                     /locus_tag="QBH27_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDQ7017077.1"
                     /translation="ISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRA
                     TGLMTSDNYRTVNNITLGKYPEAK"
     gene            204..398
                     /locus_tag="QBH27_004892"
     CDS             204..398
                     /locus_tag="QBH27_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017078.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            411..524
                     /locus_tag="QBH27_004893"
     CDS             411..524
                     /locus_tag="QBH27_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDQ7017079.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(813..959)
                     /locus_tag="QBH27_004894"
                     /pseudo
     CDS             complement(813..959)
                     /locus_tag="QBH27_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1013..1195
                     /locus_tag="QBH27_004895"
     CDS             1013..1195
                     /locus_tag="QBH27_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDQ7017080.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVTSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
BASE COUNT          363 a          283 c          313 g          287 t
ORIGIN      
        1 atttcactgt gtgatgcggt gaactttctc gtggaaaaat acgcgctggt gcgtaccgac
       61 cagccgggat tcagcgcctg tacccgctct cagttaataa acagcatcga tatcctccgg
      121 gctcgcaggg cgaccggcct gatgaccagc gacaattaca gaacggtaaa taacattacc
      181 ctgggtaagt atccggaggc gaaatgatga cactggaagc cgacagcgtt aacgtacagg
      241 cgctggatat ggggcacatt gtcgttgaca ttgatggtgt taatatcact gaactgatta
      301 ataaggccgc tgaaaacggt tattcactcc gcgtggtgga tgaccgtgac tctaccgaaa
      361 caccggcaac ttatgccagc cctcaccagt tgctgtaaga caatgcagtg atacagggta
      421 tagtgccatt aaccacagaa acagcactgt tgtcctgtat taagcaggca agagaagatg
      481 tccttaccct gcgccatctg cagcttctgc accagaaccg gtgaatctca ctcaacaggc
      541 ttcatttccc ctgacgccag cctgaataca gctggcgttt tcatttataa acagaaagga
      601 aaaccgataa taatggaatg gacgccccga ccatgaagca acggtaggat attactgata
      661 aaccttccag tggaggtgcg ttatggccgt tttaacgatt agcattgacc tgacaaaaaa
      721 tgaattccaa atccacggtc tgggtaggaa cagaaaaatc taagctcaga aaacggatta
      781 aagtaagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      841 ggcaggagtt cgtcaacacg gttggagggc cattccggca gtatgctcag aatatggcgc
      901 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      961 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
     1021 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
     1081 gacaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
     1141 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
     1201 attggttctc agatgagaat gatgacggtg atacacagat aagaat
//
LOCUS       DAOFSV010000337         1213 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.343, whole genome shotgun sequence.
ACCESSION   DAOFSV010000337 DAOFSV010000000
VERSION     DAOFSV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.343"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            19..>1213
                     /locus_tag="QBH27_004896"
     rRNA            19..>1213
                     /locus_tag="QBH27_004896"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          339 a          261 c          377 g          236 t
ORIGIN      
        1 aacatcttcg ggttgtgagg ttaagcgact aagcgtacac ggtggatgcc ctggcagtca
       61 gaggcgatga aggacgtgct aatctgcgat aagcgtcggt gaggtgatat gaaccgttat
      121 aaccggcgat ttccgaatgg ggaaacccag tgtgtttcga cacactatca ttaactgaat
      181 ccataggtta atgaggcgaa ccgggggaac tgaaacatct aagtaccccg aggaaaagaa
      241 atcaaccgag attcccccag tagcggcgag cgaacgggga ggagcccaga gcctgaatca
      301 gtgtgtgtgt tagtggaagc gtctggaaag gcgcgcgata cagggtgaca gccccgtaca
      361 caaaaatgca catattgtga gctcgatgag tagggcggga cacgtggtat cctgtctgaa
      421 tatgggggga ccatcctcca aggctaaata ctcctgactg accgatagtg aaccagtacc
      481 gtgagggaaa ggcgaaaaga accccggcga ggggagtgaa aaagaacctg aaaccgtgta
      541 cgtacaagca gtgggagcct cttaatgggg tgactgcgta ccttttgtat aatgggtcag
      601 cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta
      661 actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt
      721 gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg
      781 acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc
      841 tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg
      901 tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta tcacgggaga
      961 cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg ccagctaagg
     1021 tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc aggatgttgg
     1081 cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt cggcctgcgc
     1141 ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact atgtgttgtt
     1201 gggtagggga gcg
//
LOCUS       DAOFSV010000338         1194 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.344, whole genome shotgun sequence.
ACCESSION   DAOFSV010000338 DAOFSV010000000
VERSION     DAOFSV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.344"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(90..722)
                     /locus_tag="QBH27_004897"
     CDS             complement(90..722)
                     /locus_tag="QBH27_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDQ7017081.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
BASE COUNT          317 a          283 c          293 g          301 t
ORIGIN      
        1 cctgtttatc aggccaacgg tagaattgta atctattgaa tttacgggcc ggatacgcca
       61 catccggcac aagcattaag gcaagaaaat tattcgccgt cctgcgtttc ttctacaggc
      121 tgcatctcgc taaaccaggt atccagtttc tgccgcagct tgtccacgcc ttgtttcttc
      181 agcgaagaaa acgtttcaac ctgcacatca ccgttaaacg ccagcaccgc ttcacgcacc
      241 atattcaatt gcgctttacg tgcgccgctt gccagtttgt ccgctttggt cagcagtacc
      301 agcacggcga tattgctgtc taccgcccac tcaatcatct gctgatccaa atctttcagc
      361 ggatggcgaa tatccattag caccaccaga ccttgcaggc tctgacgttt ttcgaggtac
      421 tcgccgagcg cgcgctgcca tttgcgcttc atctcttccg ggacttccgc ataaccgtac
      481 ccaggcaagt caaccagacg cttgccgtca gccacttcaa acaggttgat aagctgggtg
      541 cgccctgggg tttttgaggt acgagccagg cttttctggt tagtcagcgt gttcagcgcg
      601 ctggatttac ctgcgttgga acggcctgca aaagccactt caattccggt atcggaaggt
      661 aggtggcgaa tatcaggcgc actcatcaca aaatgcgtct gttgataatt caaattagtc
      721 aaagcggtcg tctccatcag tcaaagcttt gcggcgatta tacctgaacc ataataaaag
      781 gcggattttt cggcgtgagc gttgtaagta aaagccatat gctttgtgag acattgccga
      841 tagtctttat gcgaaatagc agagaaaatt ctgcgacgta tgtcaaataa gctatataaa
      901 tcagtgaatt gcttttatat aaaactctga aaatgtgcaa atactgatgg cggttgattg
      961 tttatttaaa gcaaaggcgt aaagtagcac ccatagagcg aggacgctaa caggaacaat
     1021 gactcaggat gagggtcagg agcgccagga ggcgaagaca gaggattgtc aggaagataa
     1081 acgtccggag acgtaattaa atggaaatgg aatcaacacg gattgttccg gctaatggaa
     1141 aaacagggtg tgttggcggc ctgcaaggat tgtaagaccc gttaagggtt atga
//
LOCUS       DAOFSV010000339         1189 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.345, whole genome shotgun sequence.
ACCESSION   DAOFSV010000339 DAOFSV010000000
VERSION     DAOFSV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.345"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..330
                     /locus_tag="QBH27_004898"
     CDS             <1..330
                     /locus_tag="QBH27_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ7017082.1"
                     /translation="AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRL
                     RGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQ
                     QNSFKHT"
     gene            698..>1189
                     /gene="gadX"
                     /locus_tag="QBH27_004899"
     CDS             698..>1189
                     /gene="gadX"
                     /locus_tag="QBH27_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDQ7017083.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARI"
BASE COUNT          331 a          252 c          241 g          365 t
ORIGIN      
        1 gccaaactgg ggccgtatga gttcatctgt accggtcgcc cggacgaagg catcccggcg
       61 gtttgcttca aactgaaaga tggtgaagat ccaggataca ccctgtacga cctctctgaa
      121 cgtctgcgtc tgcgcggctg gcaggttccg gccttcactc tcggcggtga agccaccgac
      181 atcgtggtga tgcgcattat gtgtcgtcgc ggcttcgaaa tggactttgc tgaactgttg
      241 ctggaagact acaaagcctc cctgaaatat ctcagcgacc acccgaaact gcagggtatt
      301 gcccagcaga acagctttaa acacacctga taacatcacg ttgtaaaaac cgaatgccca
      361 acctttaaaa acaggtgggt attcggctac ttattaattg caataatata ttacctgtct
      421 attcatcgtg ttgataagat atatgttata tgaatgttat ggagcatagc gatactgctt
      481 aatattacga tgatgaatta tttgcttact attaattcct ctgcgttctt aagtcccctt
      541 tttatataat aaatccagtc atccagcccg agtatcatcg ctcattatca tcctgttttc
      601 ccgcttcgtt taaatttatt tatcaatcaa tttgacttaa gagggcggcg tgctacatta
      661 ataaacagta atatgtttat gtaatattaa gtcaactatg caaccactac atggtaattg
      721 tctaattgcg tacgcaagac ataaatatat tctcaccatg gttaatggtg aatatcgcta
      781 ttttaatggc ggtgatctgg tttttgcgga tgcaagccaa attcaagtag ataagtgtgt
      841 tgagaatttc gtattagttt caagagacac gctttcatta tttctcccga tgctcaagga
      901 ggaggcatta aaacttcatg tacataaaaa agttccttca ttactcgttc atcactgtac
      961 cagagatatt cctgtttttc aggaagttgc gcaactatcg cagaataaga atcttcgcta
     1021 tgcagaaatg ctacgtaaaa gggcattaat ctttgcgttg ttatctgttt ttcttgagga
     1081 tacgcagttt ataccgttac ttctgaacgt gttgcaaccg aatatgcgaa cacgagtttg
     1141 tacggtcatc aataataata tcgcccatga gtggacatta gcccgaatt
//
LOCUS       DAOFSV010000340         1147 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.346, whole genome shotgun sequence.
ACCESSION   DAOFSV010000340 DAOFSV010000000
VERSION     DAOFSV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.346"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..342
                     /locus_tag="QBH27_004900"
     CDS             <1..342
                     /locus_tag="QBH27_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDQ7017084.1"
                     /translation="TLHYVWAREFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAG
                     MIKQAWCSALGVDAGRYDTLAHFPVRPAVWLERDDDTGFQQVLERADYLAKESTKAYG
                     TGERNFGCSRG"
     gene            509..892
                     /locus_tag="QBH27_004901"
     CDS             509..892
                     /locus_tag="QBH27_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017085.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEGVAE"
     gene            889..1074
                     /locus_tag="QBH27_004902"
                     /pseudo
     CDS             889..1074
                     /locus_tag="QBH27_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          259 a          303 c          341 g          244 t
ORIGIN      
        1 accctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa acactatcac
       61 ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gcgattaccg cgctccggga
      121 tcactggccg ggatgattaa acaggcgtgg tgcagtgccc tgggagtgga tgccggacgt
      181 tatgacacgc tggcacattt tccggtcagg ccggcggtgt ggctggaacg cgatgatgac
      241 accggctttc agcaggtgct ggagcgtgct gactatctgg cgaaggagag taccaaagct
      301 tacggcaccg gtgagcgcaa ctttggctgc agtcgtggct gagcccgaca gacgacttat
      361 ctgaccttag tgtgatgacg tgttcacagc gaccacactg attttccctt ctgagactga
      421 actgaaaacc gccattcaca cctgaccacg ctgcccgtac cgggacggct gttgcctgtg
      481 tgtcgtctgg cgcagaggaa catatactat gtacgcaaaa tcttttctcg cacttgatgg
      541 caacggacgt ctgaccggtg cccgtaccgc acaaactgct ccttatgccc actacacctg
      601 ccacctttgc ggcagtgcac tcagatacca cccgcaatac gacaccgaac ggccctggtt
      661 tgaacacact gacgacgggc tgacggagca cgcgcagcaa tgtccttatg tcaggccgga
      721 acgcagagaa atacggttga ttaaacgtct gcagcaattc gtcccggatg ccttacccgt
      781 ggtacgtaaa gccagctggt actgcaggca atgtcaccac gattattatg gggagcagta
      841 ctgcacacac tgccagaccg ggcgtttcag tgaggagggg gtagcagaat gaacggtata
      901 aaaacggggc tggggatcac gccgggtgag cacattatca gtgccgactc tgcgctcagc
      961 agaaatatcc ggcattgttt ctgcctttcc tgtcatggcc ggttgattct gcagacagat
     1021 gctcaggggg cgtggtttga gcatgattta catgccctga gtgagcaaca gaagggtaag
     1081 cgtgcagcaa gaaccgtatt gacggggatg tgttattcag tcggcagtgc tacgcgccag
     1141 gggagca
//
LOCUS       DAOFSV010000341         1123 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.347, whole genome shotgun sequence.
ACCESSION   DAOFSV010000341 DAOFSV010000000
VERSION     DAOFSV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.347"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..173)
                     /locus_tag="QBH27_004903"
     CDS             complement(<1..173)
                     /locus_tag="QBH27_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017086.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLERE"
     gene            complement(189..674)
                     /locus_tag="QBH27_004904"
     CDS             complement(189..674)
                     /locus_tag="QBH27_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDQ7017087.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(729..>1123)
                     /locus_tag="QBH27_004905"
     CDS             complement(729..>1123)
                     /locus_tag="QBH27_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDQ7017088.1"
                     /translation="NVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQAA
                     LTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHT
                     RAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          245 a          314 c          307 g          257 t
ORIGIN      
        1 tcttcacgct ccagtcccgc catgttcaga atcagccatt cccgtgccgc acgggtggag
       61 gtgaaggcca caccgggttc atgaagatgg cggtccaggg ttttcagagc ccgcagaatg
      121 agactgcgct cgccgggcgt catttctccg ggcagaaagg aaagctgctg cattgtccct
      181 gcctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
      241 tcccgcagcc ggtaataatg gaccgtcatg gcataacact ccgtacgaca ggcgtgatga
      301 ctgtacgtca tcagacaggc agcaatgcca gcggcttccg ggctcatttc agcgcggttg
      361 ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggggcc
      421 ataaatgccc cgccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttcg
      481 cacagacggt ccatccagcc gaaaacacgg ggctccaggg ccacccactg tggaatgagg
      541 ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag ggactatggt ggcaaccaac
      601 tgaggctggt cattccctga tgcagcggtt acggaaacag aagatgtggt ggaattatgt
      661 gaaatggttt tcatgaaatg actccttaaa ggacaaataa atgcgtggtt ttcagggtaa
      721 tggctgaatc agcgcaggct ctccagcagg gtttccgcca tcacccacaa tgcgcggttg
      781 agcttaatgt cagtgtcaat gctgtgaatg gcacgggtat ggatacgttt tcctttagcg
      841 ctgcgacctg aaattccgcc tttcagcata ttctcctgaa tggtctgata agcactccac
      901 aggtccttac cgtaatcctc ccggcgtcgt ggcgtcagaa tgtcggcggt ggtgacgggc
      961 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
     1021 ggcggcagaa tcagcgactg catggcatca cgcttttcct caatccggtc aaacacgccc
     1081 actacctcgt aagccccttc aatgactctg tccaccacat ttc
//
LOCUS       DAOFSV010000342         1108 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.348, whole genome shotgun sequence.
ACCESSION   DAOFSV010000342 DAOFSV010000000
VERSION     DAOFSV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.348"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            52..>1108
                     /locus_tag="QBH27_004906"
     CDS             join(52..301,301..>1108)
                     /locus_tag="QBH27_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
                     /protein_id="HDQ7017089.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAASAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRARYPVATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATQRGYQMGRWLAGRLMKELGLVSCQQPTYRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPVGVMFHSDQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLKNEWV
                     PATGYVSFSDAAHAITDY"
BASE COUNT          303 a          275 c          310 g          220 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgccat aatgaaaaaa
       61 agaaatttca gcgcagagtt taaacgcgaa tccgctcaac tggtcgttga ccagaattac
      121 accgtggcag atgcagccag cgctatggat gtcggccttt ccacaatgac gcgatgggtg
      181 aaacaattac gtgatgagcg gcagggaaaa acaccaaaag cctcccctat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgta ttgaaatgga aaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagggc gcgttatcct gtggccactc tctgccatgt gttcggggtc catcgcagca
      421 gctacaaata ctggaaaaac cgtcctgaaa agccagacgg cagacgggct gtattacgca
      481 gccaggtact tgaactgcat ggcatcagcc acggctctgc cggagcaaga agcatcgcca
      541 caatggcaac ccagagaggc taccagatgg ggcgctggct tgctggcaga ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga cttaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaacggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc
      841 ttaccatgaa agcactggaa atggcatggg aaacccgtgg taagcccgtc ggggtgatgt
      901 tccacagcga tcaaggcagt cattatacga gcaggcagtt ccggcagtta ctgtggagat
      961 accggatcag gcagagtatg agtcggcgtg gaaactgctg ggataacagc ccaatggagc
     1021 gcttcttcag gagtctgaag aacgaatggg tgccggcgac gggctatgta agcttcagcg
     1081 atgcagctca cgcaataacg gactatat
//
LOCUS       DAOFSV010000343         1103 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.349, whole genome shotgun sequence.
ACCESSION   DAOFSV010000343 DAOFSV010000000
VERSION     DAOFSV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.349"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            1..807
                     /locus_tag="QBH27_004907"
                     /pseudo
     CDS             1..807
                     /locus_tag="QBH27_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642102.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          327 a          150 c          187 g          439 t
ORIGIN      
        1 aggctttaac atttaaagtt gtactttcaa tatatagcat cttaataatg gcatacgtct
       61 cctcttgtta tgcacagacg ttgtggttat gttctgaaga aatacttaag agatccgtct
      121 tttatttgtt cgcatttctt tgccttattg gcattataag tattctttta cagaagactg
      181 agattataca tgataaaagt atgattcttt ttcctgaacc atcagcattt gcattggttt
      241 ttatacctat cttttcattt tgtttatact atacaagagg gggggggcta ctattgctct
      301 atatattatc tttgggtatt gcgttaggta tccagaattt aacaatgttg gtaggcattg
      361 tgattagtgt ttttgtgatg aaaaaaataa ctataaggca aactattgtt atacttttgg
      421 gggcatggat tttttccatg atattaagtg atttagacat ttcttactat acatcgcggc
      481 ttgattttaa aaatactacg aacctatcag tgcttgtata tctttcagga attgaaagag
      541 ctttcttgaa ttttattaca agttatggtc ttggtattgg ttttcaacaa atgggagtga
      601 atggggagat aggaatatat caacaaattt tagctgaact tgatgcccct atgttaaata
      661 tatacgatgg ctcatttatt tcttctaagt taatatctga gtttggggtt attggtgcat
      721 taatgtgtat tttctatttt ttttattttt cccgatttta tctgcgtttc aaaaaaagta
      781 agagatattc accgcagtat attttagcat atagcttcta catgtgtttc ttcatccctc
      841 tttttatacg tggtgctggt tatataaacc cctatgtgtt tatgttattt tcatcaatat
      901 ttttgtgcaa atatcacgct aaaaatatct tgatgaaatc taatgtccag atagctatat
      961 aatagtagat tatattatca ttatcacgta aattacatat taatagcata tatgataact
     1021 aggacataaa taatgtgcat taaaaaaaaa cttaagttaa ttaaacgata tggcctttat
     1081 ggtggtctta ggcttcttaa aga
//
LOCUS       DAOFSV010000344         1087 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.350, whole genome shotgun sequence.
ACCESSION   DAOFSV010000344 DAOFSV010000000
VERSION     DAOFSV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.350"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..498
                     /locus_tag="QBH27_004908"
     CDS             <1..498
                     /locus_tag="QBH27_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296213.1"
                     /note="WbbJ; catalyzes the transfer of the O-acetyl moiety
                     to the O antigen; part of the lipopolysaccharide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDQ7017090.1"
                     /translation="RIIRFPCYIRKDGSVSFGKGFTSGVGLRVDAFMDAVVSIGENVQ
                     INDYVHIAAINNVIIGRDTLIASKVFISDHNHGIFSKSDIHSSPTIIPSSRPLESAPV
                     YIGERVWIGENVTILPGACIGNGVVIGANSVVRGEIPNNVIIAGVPAKIVKKYNYERM
                     QWERI"
     gene            483..>1087
                     /locus_tag="QBH27_004909"
     CDS             483..>1087
                     /locus_tag="QBH27_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDQ7017091.1"
                     /translation="MGKNIVVISAVNFTTGGPFTVLKNVLTATKDRAECKFIALVHSS
                     AELMELFPWVEFIEYPEVKSSWVKRLYFEYITCNRLSKVIKATHWVCLHDITANVSVP
                     YRFVYCHNPAPFYKYLSYRDIIGEPKFYLFYLFYGLLYNINIKKNTAVFVQQQWLKKE
                     FEKKYKLKNVVVSRPEDICPFESDGLVRNNNKKDVRIFYPAV"
BASE COUNT          351 a          146 c          220 g          370 t
ORIGIN      
        1 aggattatta gatttccatg ttatattaga aaagatggaa gtgttagttt tggaaaaggt
       61 tttacatcag gtgtaggatt acgagttgat gcatttatgg atgccgtagt ttccattgga
      121 gaaaatgttc aaattaatga ctatgttcac atcgcggcta ttaataatgt cattattggt
      181 agagatacat taatagcaag taaagtattt attagtgatc ataatcatgg tattttttct
      241 aaatccgata tccatagttc accaactatt attccttcgt ctaggcccct tgaatctgca
      301 cctgtgtata ttggagagcg tgtgtggatt ggcgaaaatg tgacaatatt accaggtgcg
      361 tgtataggta atggtgtagt tattggcgca aacagtgttg ttcgtggtga gattcctaat
      421 aatgtgatca ttgctggtgt tccagctaaa attgttaaaa aatataacta tgagcgtatg
      481 caatgggaaa gaatatagtt gtaatatcgg ctgttaattt tacaaccgga ggccccttta
      541 ccgtactaaa aaatgtgctt acagcaacta aagatagagc cgaatgtaaa tttattgcac
      601 tggttcatag ctctgctgaa ctaatggaat tatttccgtg ggttgaattt atagagtatc
      661 cagaagtcaa gtcttcgtgg gttaaaagat tatatttcga atatataact tgcaatagat
      721 tatctaaggt gattaaggca actcattggg tatgcttaca tgatattaca gcaaatgtta
      781 gtgtacccta tagatttgtt tattgccaca atcctgcacc gttctataaa tatttaagct
      841 atcgagatat tataggagaa cctaaatttt atctttttta tcttttttat gggcttttat
      901 acaatatcaa tataaaaaag aacacagcag tttttgttca gcagcagtgg ctaaaaaaag
      961 aattcgaaaa aaaatataag ttaaagaatg ttgttgttag tcgccctgaa gatatttgcc
     1021 cttttgaaag tgatggtttg gtaagaaata ataataaaaa ggatgtgagg atattttacc
     1081 cagcagt
//
LOCUS       DAOFSV010000345          983 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.354, whole genome shotgun sequence.
ACCESSION   DAOFSV010000345 DAOFSV010000000
VERSION     DAOFSV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.354"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            443..658
                     /locus_tag="QBH27_004910"
     CDS             443..658
                     /locus_tag="QBH27_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017092.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            800..983
                     /locus_tag="QBH27_004911"
                     /pseudo
     CDS             800..983
                     /locus_tag="QBH27_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          284 a          261 c          225 g          213 t
ORIGIN      
        1 accagccctg tggtaaaata cagtaatagc ggcgtacatc aggcacgccg ctcactcgtc
       61 cacagatcat atacgtcatt atactgcgcg ataacggagg gcttcagcac ccggtcattt
      121 ctgagcgggt atgaatacca ttgatgatgc gaagcagcca cgggcagaaa cgttcttcac
      181 aaccaggtgc ctccatcttc actgcaggct gaatgtgccg gcttgttatc caccagaaat
      241 ggtatgctcc ggtacatgca taccccggag actataaacg cactgaatca tagcctccca
      301 cctcagcagg aggcagtcag ctttcaggcg gaaaaacgtt gcagttaaag atcgtgagaa
      361 tcgctgtttc gttgcatagc aacccgaaca gttgtagttg aaacatttta cctgcatcag
      421 cacatggtca ggcgccgtct gtatggcgtg gaatactgat gtttcggctg atggcaagcc
      481 tcattctctg tggaaacagg ggcgcgcatt agcttctcaa ccagagagtg tcacaaagac
      541 aaatattgct gaaaatcgca ttcatcgttc aggcgtgaat gcccggagac aaaagccgct
      601 gtccgcacag cccccctctg atctgaacaa aaagcataca cacaacaggc tcacctgacc
      661 ccgaaaaacc ataacttcag caataccagt gacaccagca cgttacctca gtgccacatc
      721 ttcagaccgc gatcaatctg aaatgtgact atcggggcaa tatcccagtt ttgtggtaca
      781 cgataaccag aaaacatccc ctgaaccgcc agccagaaag accggccaca aaagggttaa
      841 gctgaaatcg tttatggatt acatcgacat atgcctggca gagaatgtcc ggaatgacga
      901 ggttatcttt gcggagatta aggcaatgtg tcaacgacgg atgaaaagtg atccacttat
      961 atctccacca acggcccaat att
//
LOCUS       DAOFSV010000346          938 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.355, whole genome shotgun sequence.
ACCESSION   DAOFSV010000346 DAOFSV010000000
VERSION     DAOFSV010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.355"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..>938)
                     /locus_tag="QBH27_004912"
     CDS             complement(<1..>938)
                     /locus_tag="QBH27_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ7017093.1"
                     /translation="RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLL
                     IPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPAL
                     DLSRLPELLSRISSYKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAE
                     REPLPGVKFSHRGSKMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISE
                     NTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAY
                     IHKAEHLEERRLMLQWWADFLDANREKGISPFEYAK"
BASE COUNT          227 a          234 c          230 g          247 t
ORIGIN      
        1 atctttgcat attcaaacgg actgataccc ttctcccggt tcgcatccag aaaatcggcc
       61 caccattgca acatcagtcg ccgttcttcc agatgttctg ctttatggat atacgcagca
      121 cgtacaccat tacgctcctg atggctcatc tgacgttcca cagcatcgcg ggaccacaaa
      181 cctgattcaa tcaatgcact gcacgccatc gcccggaatc catggccaca gacttcctgg
      241 gttgtgtcat accccatcac cctcagggcc ttatttacag tattttcact gattggttta
      301 cgcgggtcat gtgcacccgt aaatatcaga ccattttcac cagcccatgt ctgaagttct
      361 gtcagtatgg ctacagcctg tttgctgagt ggcacaagat gtggtgttcg catcttggag
      421 ccccggtgtg agaattttac gccgggcaga ggttcgcgtt cggctggaat agtccacatg
      481 gcattgtcaa tatcaatttc agaccaccgg gcgtatctga gttcactgga acgaataaaa
      541 accagtaaat tcagcataac ggcaagctgg gtgacaggtt gccccttgta actgctaata
      601 cgcgacaaaa gttcaggcag gcgagaaaga tcaagagcgg ggcgatgctg gcgttttacc
      661 gtggtcaatg cgccagccat atccagagca ggattatatc tgatgatgcc agactgtacg
      721 gcataacgca ttacggcaga tattctttgc tgcagacgac tggcgatttc ataaatttgt
      781 ttagcttctg cagcacgaac agggataagc agatccgggg tattgagtgt tgtgatatcc
      841 cggttgccga taaaggggaa aacgtgagtc tcgaggcttt tgagtacttt atcgctgtgt
      901 gattgtgccc attgtttatt actggcatgc cagcgtcg
//
LOCUS       DAOFSV010000347          906 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.356, whole genome shotgun sequence.
ACCESSION   DAOFSV010000347 DAOFSV010000000
VERSION     DAOFSV010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.356"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(68..763)
                     /gene="aqpZ"
                     /locus_tag="QBH27_004913"
     CDS             complement(68..763)
                     /gene="aqpZ"
                     /locus_tag="QBH27_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDQ7017094.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT          297 a          243 c          194 g          172 t
ORIGIN      
        1 atgatgaatc aacatataac aacatcttaa aaaaaggcct gacattgcgc caggccttct
       61 gcgttaatta attacgcttt tccagtagag tccggtaaat cagaccaccg ataataccgc
      121 cgacaattgg caccacccag aagaaccaca gttgttctaa tgcccagccg ccctggaaga
      181 tagcaaccgc agtactgcgc gccgggttaa cggaagtgtt agtcaccgga atactgatta
      241 agtgaatcag ggttaaggcc agaccaatgg caattggcgc aaaaccagca ggagcgaatt
      301 tgtcagttgc gccgtggatc accagcagga aacctgcact caataccagt tcaactacca
      361 gcgcggaaag catggaataa ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac
      421 cgctggctgc cgcgtcaaaa cccgttttac cactggcaat taaatacagc agcgccgccg
      481 caacaatacc gccgacaacc tgggcaatta cgtagccaac gacttctttt gccgggaaac
      541 gtccgccagc ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac
      601 caacagcaaa ggccattgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac
      661 caatgcctaa ttccgggaag cctgcggcca gtacggcact accacagccg ccaaaaacaa
      721 gccagaaagt accaaaacat tcagctgcta attttctgaa catatccacc tcaattaaaa
      781 attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa
      841 ataatgcgcg gctattttaa aaacgaaggc aaatcattcg ccagataaat aaatccagta
      901 aatttg
//
LOCUS       DAOFSV010000348          902 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.357, whole genome shotgun sequence.
ACCESSION   DAOFSV010000348 DAOFSV010000000
VERSION     DAOFSV010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.357"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            32..902
                     /locus_tag="QBH27_004914"
                     /pseudo
     CDS             32..902
                     /locus_tag="QBH27_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          261 a          216 c          221 g          204 t
ORIGIN      
        1 ggacataatc tgacatctga aggtgcagtt tatgaaacat tcatttgaag taaaacttgc
       61 cgctgtaaat cattacctgg ctggtcatgc aggcatcatt tctacggcaa aactctttca
      121 actttctcac accagcctct cacactggat taatcttttt cttcttcacg gtcctcgggc
      181 actggattgc agacacaagc gtagctattc tcctgaagat aaactttgcg tggttcttta
      241 tgctctcggg cattctgagt ctctaccccg ggtagcagcc cggtttaaca ttcccagcca
      301 caacacggtt aaaaactgga taaaaggcta ccgcaaatct ggtaatgagg cctttatcag
      361 gcgcaggaag gagaaaagca tgacccgttc tgatgatacc catgaaaacg aggcaaacat
      421 gacgccggaa gagatgaaga atgagctgcg ttatcttcgc gcagaaaatg catatctgaa
      481 agccatgcag gagcatcttc tggaaaaaaa gcgccaggag ctggagaaaa aacgaaagtc
      541 atccagagcc tgaggtacgg acactgccag tctgacctgt taaaggccgc agggctggcc
      601 agaagcacgc tgtactacca gctgtcactg cagaaggcta aggacaagta tgctgatgtg
      661 aaacagctca tagcctccat cttccatgaa caccggggat gttatgggta ccggcggatc
      721 cattgtgagc ttcagaaaag aggtcttaag ttcagtggca aaacggtgcg aaaactgatg
      781 caacagctcg gcctgaagtc tcctgtgcgc ctgaagaaat accggtctta ccggggaaat
      841 atgggactcg ctgcagaaaa tatccttcag cgacagttca aagcggaagc tccctgtgag
      901 aa
//
LOCUS       DAOFSV010000349          860 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.358, whole genome shotgun sequence.
ACCESSION   DAOFSV010000349 DAOFSV010000000
VERSION     DAOFSV010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.358"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>860
                     /locus_tag="QBH27_004915"
     CDS             <1..>860
                     /locus_tag="QBH27_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HDQ7017095.1"
                     /translation="IDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAIS
                     NGGTGTQINGDEATVNNNGNTTVDGQGSTGTEIAGNNVVVNQDGTLDVSGGGHGIDIT
                     GDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVNNN
                     GNTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPD
                     SIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIAGN
                     NAVVNQDGTL"
BASE COUNT          234 a          227 c          259 g          140 t
ORIGIN      
        1 attgatatca ccggcgacag cgccacggtg gacaacaagg gcggtatgac ggttaccgat
       61 ccggattcca ttggtattct gatcgacggt gataaagcca tcgtcaacaa cgacggcgac
      121 aatgccatca gcaacggcgg caccggcacg cagattaatg gcgatgaagc cactgtaaac
      181 aataacggta acaccaccgt tgatggtcag ggctctacag gtaccgaaat cgcgggtaac
      241 aacgtggtgg tgaatcagga cggtacgctg gatgtcagcg gcggcggtca cggtattgat
      301 atcaccggcg acagcgccac ggtggacaac aagggcggta tgacggttac cgatccggat
      361 tccatcggta ttctgatcga cggcgacaaa gccatcgtca acaatgacgg cgataacgcc
      421 atcagcaacg gcggcaccgg cacgcaggtt aacggtgatg aagccactgt aaacaataac
      481 ggcaacacca ccgttgatgg tcagggctcg accggtaccg aaatcgcggg taataacgct
      541 gtagtgaatc aggacggcac gctggatgtc agcggcggcg gtcacgggat tgatatcacc
      601 ggcgacagcg ccacggtgga taacaaaggc ggtatgacgg ttaccgatcc ggactccatc
      661 ggtattctga tcgacggcga caaagccatc gtcaacaacg acggcgacaa cgccatcagc
      721 aacggcggca ccggcacgca ggttaacggt gatgaagcca ccgtgaacaa taacggtaaa
      781 accaccgttg atggtcaggg ctcgaccggc acggaaatcg cgggtaataa cgctgtagtg
      841 aatcaggacg gcacgctgga
//
LOCUS       DAOFSV010000350          784 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.359, whole genome shotgun sequence.
ACCESSION   DAOFSV010000350 DAOFSV010000000
VERSION     DAOFSV010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.359"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>784
                     /locus_tag="QBH27_004916"
     CDS             <1..>784
                     /locus_tag="QBH27_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
                     /protein_id="HDQ7017096.1"
                     /translation="MPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARA
                     QRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGK
                     KVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIV
                     GWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTG
                     STGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD"
     misc_feature    <1..72
                     /locus_tag="QBH27_004916"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          189 a          190 c          241 g          164 t
ORIGIN      
        1 atgccactgc tggataagct gcgtgagcag tacggggtcg gaccgctatg cagcgaactg
       61 catattgccc cgtcaacgta ttaccactgt cagcaacagc gacatcatcc ggataaacgc
      121 agtgcccgtg cgcagcgcga tgactggctg aagaaagaga tacagcgcgt atacgatgaa
      181 aatcacaagg tatacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggtatcaga
      241 gtggccagat gcactgtggc acgtctcatg gcggttatgg gacttgccgg tgttctccgg
      301 ggtaaaaagg tccgtacgac catcagccgg aaagccgttg ccgcaggcga ccgcgtaaac
      361 cgtcagttcg tggcagaacg acctgaccag ctgtgggtgg ctgattttac ttacgtcagc
      421 acatggcggg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg atacatcgtg
      481 gggtggcggg tctcatcgtc catggaaacg acattcgtgc tggatgcact ggagcaggcg
      541 ttatgggccc gtcgaccgtc cggcacggtc catcacagtg ataaaggttc tcagtatgta
      601 tcgctggcct acacacagcg gcttaaggaa gccggattac tggcatcaac aggaagtaca
      661 ggcgactcgt atgacaacgc gatggcggag agcatcaatg gtctttacaa agcggaggta
      721 atacaccgta agagctggaa aaaccgtgca gaagtggaac tggccacact cacgtgggtg
      781 gact
//
LOCUS       DAOFSV010000351          763 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.360, whole genome shotgun sequence.
ACCESSION   DAOFSV010000351 DAOFSV010000000
VERSION     DAOFSV010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.360"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            223..763
                     /gene="papA"
                     /locus_tag="QBH27_004917"
                     /pseudo
     CDS             223..763
                     /gene="papA"
                     /locus_tag="QBH27_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
BASE COUNT          215 a          125 c          195 g          228 t
ORIGIN      
        1 tcggcatttg actaaattat ggcattccgg agtttctgga agataaaaaa agaagccctt
       61 gtcagaaagc agacaggtta tatcagtatt ctgtcgataa ataacctgcc ctgaaaatac
      121 gagaatatta tttgtattga tctggttatt aaaggtaatc gggtcatttt aaattgccag
      181 atatctctgg tgtgttcagt aatgaaaaag aggttgttat ttatgattaa gtcggttatt
      241 gccggtgcgg tagctatggc agtggtgtct tttggtgtaa atgctgctct aactactcca
      301 caggggcagg gtagagtaac ctttaacgga actgttgttg atgctccatg cagcatttct
      361 cagaaatcag ctgatcagtc tattgatttt ggacagcttt caaaaagctt ccttcaagca
      421 ggaggcgtat ccaaaccaat gaatttagat attgaattgg ttaattgtga tatcacttca
      481 tttaaggggg gggaggagtc tcggcagcaa aaaaagggac tgtgaagctg gcttttagtg
      541 gtccaagggt ttctggtcat aatgaggagt tagataccag cggggggaca ggtactgcga
      601 ttgtagttca ggctgcaggt aaaaacgttt ctttcgatgg cacagaaggt gatgctaata
      661 ccctgaaaga tggtgataat gtgctgcatt atactgcgat tgttaagaag tcgtcagcca
      721 ataatgccca agtcactgag ggtgcttttt ctgcagtcgc aac
//
LOCUS       DAOFSV010000352          762 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.361, whole genome shotgun sequence.
ACCESSION   DAOFSV010000352 DAOFSV010000000
VERSION     DAOFSV010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.361"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            221..409
                     /locus_tag="QBH27_004918"
     CDS             221..409
                     /locus_tag="QBH27_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ7017097.1"
                     /translation="MSQYPELIAQFSTGNQTRIKQGLIAKAPLEGWHYGSKEIVKEFH
                     IYHSVAIECGGEIYDIDN"
     gene            463..753
                     /locus_tag="QBH27_004919"
     CDS             463..753
                     /locus_tag="QBH27_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDQ7017098.1"
                     /translation="MHDLKYKIYFYTEGEFLEMLEEIVNATSKDKSLKGKKLEKYLDT
                     LVDHFIKITEHPKKGDLIFYPNSQEDGEPENILKIVKEWRRSQGLPLFKDSK"
BASE COUNT          277 a          119 c          146 g          220 t
ORIGIN      
        1 gtcaaagaat ggcggcgttc acaggggcta cccttgttta aagactctaa ataaagtaga
       61 taataaaact atatatggaa catcatcgcg tctggctaat gcgatgatgt taataacata
      121 aaataataat gtgggtgctc ttaaaaaaca ggtaaaatac tgaaatttca ttaggttatt
      181 ttatcaattt cccgctttca ccgataccct ttggctggag gtgagtcagt atccggagtt
      241 gatagcgcag ttttcaactg gtaaccaaac acgtataaaa caaggattaa tcgcaaaagc
      301 gcctcttgaa ggttggcatt atggctctaa ggaaattgtt aaagaattcc atatatatca
      361 tagtgttgcg attgaatgtg gtggcgaaat ttatgatatt gataattaaa ggattgttac
      421 gcctcgacta catcatgaaa ttcactacag gagataatgt atatgcatga tttaaaatat
      481 aaaatatatt tttacacaga aggtgaattt cttgaaatgc ttgaggagat agtaaatgca
      541 actagcaaag ataaatccct aaaagggaaa aaattagaaa aatatctcga taccttagtg
      601 gatcatttca ttaaaattac tgaacatcct aaaaagggag atctcatttt ctatcctaat
      661 tcccaagaag atggtgaacc tgaaaacatc ctgaaaatcg tcaaagaatg gcggcgttca
      721 caggggctac ccttgtttaa agactctaaa taaagtagat aa
//
LOCUS       DAOFSV010000353          709 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.362, whole genome shotgun sequence.
ACCESSION   DAOFSV010000353 DAOFSV010000000
VERSION     DAOFSV010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.362"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..282)
                     /locus_tag="QBH27_004920"
     CDS             complement(<1..282)
                     /locus_tag="QBH27_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
                     /protein_id="HDQ7017099.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQV"
     gene            complement(310..489)
                     /locus_tag="QBH27_004921"
     CDS             complement(310..489)
                     /locus_tag="QBH27_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDQ7017100.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(494..>709)
                     /locus_tag="QBH27_004922"
     CDS             complement(494..>709)
                     /locus_tag="QBH27_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDQ7017101.1"
                     /translation="ATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELAD
                     LTVGAATGEISVSSVADTLRRLYGSAE"
BASE COUNT          172 a          207 c          148 g          182 t
ORIGIN      
        1 gacctgcccc tctttcctgc gcaaccacgc taccagcgag tcaattccac caatacggcg
       61 aaacagttca tcgctgttaa aaaacggctg caacgcctca tttgttgcca tagtttgctc
      121 ggaaacaacg aggccgtcgt ccatgtgctc gattaactca cgcggtaccg gctccataat
      181 aaatttacgg ccagcctcca ccagctttct gacttcctga tccactttga atgtggcgac
      241 gccaagctct ttttgtacaa agggagtaat tacggctttc acatcacacc tttcatcact
      301 gattgggctt tatctgctgc ccggcattct ctgtttaagc acaaccattt cctgacggca
      361 taacacagca atagcggtcc tggcaccaat ttgcttacca gccaggtatt gctttacctc
      421 gcggcgactc acgccatcaa gaagcatctt taacgcttca cgggacaatt tgttgtattt
      481 gcgtgccatt aatctactcc gcagaaccat acaatctacg taacgtgtcg gcgacagaag
      541 atacagatat ctctccggtc gcagccccta cggtaaggtc tgccagttca ggtgaatcaa
      601 atacctgcac cccgttacgg cgtagaaata acagcgcact gtttagcgcg gtacgcttat
      661 tggcatcatt gaatatatgc cctctcgctg tagccaccag gtaggtggc
//
LOCUS       DAOFSV010000354          698 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.363, whole genome shotgun sequence.
ACCESSION   DAOFSV010000354 DAOFSV010000000
VERSION     DAOFSV010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.363"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>698
                     /gene="tuf"
                     /locus_tag="QBH27_004923"
     CDS             <1..>698
                     /gene="tuf"
                     /locus_tag="QBH27_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ7017102.1"
                     /translation="ATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
                     MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLD
                     EGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFK
                     GYRPQFYFRTTDVTG"
BASE COUNT          162 a          170 c          199 g          167 t
ORIGIN      
        1 gcgactgacg gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc
       61 gttccgtaca tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg
      121 gaactggttg aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac
      181 actccgatcg ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg
      241 aaaatcctgg aactggctgg cttcctggat tcttacattc cggaaccaga gcgtgcgatt
      301 gacaagccgt tcctgctgcc gatcgaagac gtattctcca tctccggtcg tggtaccgtt
      361 gttaccggtc gtgtagaacg cggtatcatc aaagttggtg aagaagttga aatcgttggt
      421 atcaaagaga ctcagaagtc tacctgtact ggcgttgaaa tgttccgcaa actgctggac
      481 gaaggccgtg ctggtgagaa cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc
      541 gaacgtggtc aggtactggc taagccgggc accatcaagc cgcacaccaa gttcgaatct
      601 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
      661 cgtccgcagt tctacttccg tactactgac gtgactgg
//
LOCUS       DAOFSV010000355          678 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.364, whole genome shotgun sequence.
ACCESSION   DAOFSV010000355 DAOFSV010000000
VERSION     DAOFSV010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.364"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            249..458
                     /gene="fumD"
                     /locus_tag="QBH27_004924"
     CDS             249..458
                     /gene="fumD"
                     /locus_tag="QBH27_004924"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDQ7017103.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          216 a           98 c          149 g          215 t
ORIGIN      
        1 aaattgttat caaagattca gcgttccagg gaaaaggtta cgcaaaaact tcgttgtttg
       61 cgcaacgtct ggtgccgggg ttgtcagtgt gaggataagt gaaagttcat ccggtgtata
      121 aatacatcat cgctcaattg ctacagttaa aataatttat cacgttattt gtctgatttt
      181 gtttgatttt tgtttaattg gttagattga tatatgctga tagttggcat gataaatagg
      241 tataaagaat gggaaaccgg acaaaagagg atgaactgta ccgagaaatg tgcagagttg
      301 tcggtaaagt tgtgctggaa atgcgcgatc tggggcagga accaaagcat attgttatcg
      361 caggcgtatt acgtacagca ttagccaata aacgcattca gcgcagtgag ctggaaaagc
      421 aggcgatgga aacagtcatt aacgcactag taaaatgacc attcaggatg aggcggtaag
      481 cttcatcctg atattgttag ttcagttgcg gtccagttaa tcattctaaa taacaactgc
      541 tgaaattata cgaatttcgg tgtatatttt aatttatagc gataaagtta tttttatttt
      601 ctgcgatgaa tttcacaaat aattgtcggt taagttaaat ttatttcaaa tgataataaa
      661 tatcacttca cttataat
//
LOCUS       DAOFSV010000356          676 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.365, whole genome shotgun sequence.
ACCESSION   DAOFSV010000356 DAOFSV010000000
VERSION     DAOFSV010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.365"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            137..595
                     /gene="tnpA"
                     /locus_tag="QBH27_004925"
     CDS             137..595
                     /gene="tnpA"
                     /locus_tag="QBH27_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HDQ7017104.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKCRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          207 a          124 c          190 g          155 t
ORIGIN      
        1 gaaaaccccc agctaggctg ggggttccgg aaagctttca gctttgagcc agttattaaa
       61 accccttttg atttgttaaa acaccttgcg gtctggcaac tgcaagtgtc aaacaagaaa
      121 tcaaaagggg gtcccaatgg ggaacgaaaa gagcttagcg cacacccgat ggaactgtaa
      181 atatcacata gtatttgcgc caaaataccg aagacaggtg ttctacagag agaagtgtag
      241 agcaataggc tgtattttga gaaagctgtg tgagtggaaa agtgtacgga ttctggaagc
      301 tgaatgctgt gcagatcata tccatatgct tgtggagatc ccgcccaaaa tgagcgtatc
      361 aggctttatg ggatatctga aagggaaaag cagtctgatg ccttacgagc agtttggtga
      421 tttgaaattc aaatacagga acagggagtt ctggtgcaga gggtattacg tcgatacggt
      481 gggtaagaac acggcgaaga tacaggatta cataaagcac cagcttgaag aggataaaat
      541 gggagagcag ttatcgattc cctatccggg cagcccgttt acgggccgta agtaacgaag
      601 ttggatgcaa atgtcagatc gtgtgcgcct gttagggcgc ggctggtaag agagccttaa
      661 aggcgcattt gaaaaa
//
LOCUS       DAOFSV010000357          647 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.366, whole genome shotgun sequence.
ACCESSION   DAOFSV010000357 DAOFSV010000000
VERSION     DAOFSV010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.366"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..368)
                     /locus_tag="QBH27_004926"
     CDS             complement(<1..368)
                     /locus_tag="QBH27_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDQ7017105.1"
                     /translation="MSKDYMADGSLTEKWKYRFSFYDEHGFPGFWGASPEYKQALKGL
                     NTRQKIIIRTNVIAFFFSWVYLFVLGLWKKAIVVLFLAIFSLIFGYMIGYNVFGFIIA
                     AYVSYNTNRWFYEKEVKGIHT"
     gene            complement(383..>647)
                     /locus_tag="QBH27_004927"
     CDS             complement(383..>647)
                     /locus_tag="QBH27_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017106.1"
                     /translation="GAGFFVITSTILLHYLVINMNCQRLRDSGFTYIKTYIFSTLVVY
                     IISFIIMVAEHFDCSGNGSMIFLICYFSTFGMLVLAPTDSPRK"
BASE COUNT          249 a          120 c          101 g          177 t
ORIGIN      
        1 gtatgaatac cttttacttc tttttcatag aaccatctgt tggtattgta tgaaacataa
       61 gcggcgataa tgaatccaaa tacattatag ccaatcatgt aaccaaaaat taaagaaaat
      121 atcgccagga atagaacaac aatagccttc ttccataaac ccaatacaaa taaatagacc
      181 cacgagaaga aaaaagcaat aacatttgtt ctaattatta tcttttgcct tgtgtttaat
      241 ccttttaatg cttgtttata ttctgggctt gcaccccaaa atccagggaa gccatgttca
      301 tcataaaacg aaaagcggta cttccacttt tcagttaatg agccatctgc catataatcc
      361 ttactcataa aatctccatt aattatttac ggggggaatc agtgggggca agaactaaca
      421 tgccaaaggt ggaaaaataa caaatcaaaa aaatcatcga accattgcca gaacagtcaa
      481 aatgctcagc aaccatgatg atgaaagaaa tgatatagac tactaatgta ctaaaaatat
      541 aagtttttat ataagtaaaa ccactatccc ggagtcgttg gcaattcatg ttgataacga
      601 gataatgtaa aagaatggta gatgttataa caaagaaacc agccccg
//
LOCUS       DAOFSV010000358          615 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.368, whole genome shotgun sequence.
ACCESSION   DAOFSV010000358 DAOFSV010000000
VERSION     DAOFSV010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.368"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..610
                     /locus_tag="QBH27_004928"
     CDS             <1..610
                     /locus_tag="QBH27_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDQ7017107.1"
                     /translation="TLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAFLALLPF
                     LAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLT
                     VSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDW
                     QAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT          169 a          102 c          128 g          216 t
ORIGIN      
        1 tactttatat agtctggtaa tgagttttct ctggagcaat accagtttcg gtatacatcg
       61 tttcaaggtg aaattagata ctacgtattg tataaaatat gccattctcg catttttagc
      121 tttattacct tttctcgctg ttgctggtta tattatcttc gatcaaatat taaatgcgta
      181 tgatagttct gtgtatgcaa atgatgatat tgagaattta cagcaattta tggaaatgca
      241 acgtaaaatg ataatcgcgc agttaatcta ttattttggg attgctgtta gcaccagtta
      301 tttaacggtg tcgttgcgaa atcattttat gagcaacctg tcactgaatg atgggcgtat
      361 tcgttttcgc tcaactttaa cataccacgg tatgctttat cgcatgtgtg cgttggtggt
      421 gatatccggg attacgggcg gtctggctta tccactgctg aaaatatgga tgattgactg
      481 gcaggcaaaa aatacgtatt tgctgggcga tttggatgac cttcctttaa tcaataaaga
      541 agaacaacca gataaaggct tcttagccag tatttcacgg ggaattatgc cttctttacc
      601 atttctgtaa gagat
//
LOCUS       DAOFSV010000359          590 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.369, whole genome shotgun sequence.
ACCESSION   DAOFSV010000359 DAOFSV010000000
VERSION     DAOFSV010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.369"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..558
                     /locus_tag="QBH27_004929"
     CDS             <1..558
                     /locus_tag="QBH27_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDQ7017108.1"
                     /translation="KIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAII
                     EVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYS
                     FTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT          159 a          146 c          154 g          131 t
ORIGIN      
        1 aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa
       61 gtacaggctt cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact
      121 caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat
      181 gatgaagatt accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc
      241 gttgccagag ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt
      301 caggaagatc aactgggcgc actggggctg gtcactaacg ccgtcgtgtt atggaacact
      361 atttatatgc aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa
      421 gatatcgcac gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc
      481 ttcacgctgg cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag
      541 gcagaaaacg ttgcttaacg tgagttttcg ttccactgag cgtcagaccc
//
LOCUS       DAOFSV010000360          583 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.370, whole genome shotgun sequence.
ACCESSION   DAOFSV010000360 DAOFSV010000000
VERSION     DAOFSV010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.370"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            174..583
                     /locus_tag="QBH27_004930"
                     /pseudo
     CDS             174..583
                     /locus_tag="QBH27_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238190.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          135 a          155 c          164 g          129 t
ORIGIN      
        1 gttgggtgta gtgtaagcga ctgaaaaaca aacgcccctg aaatcatgtc cagatccggc
       61 agaaccagcc tcatgcgcag ttctggctgg tttttgacgt tgatcgcgag ggggcagcga
      121 ttgactggag cgaccggaac gctcccgcgc cgaacatcac cgtaaaaaat cctgtgaacg
      181 gtcatgctca cctgctctac gcgctcaata tcgccgtgag aaccgcgccg gacagctcgg
      241 ttaaggcgct gaaatacgcc gctgcgattg aacgttcgct gtgtgagaaa ctttgtgctg
      301 atgtgaacta cagcggactg atttgtaaaa atccgttcca cctggaatgg caggtgatgg
      361 agtggcgaga ggaagcctat accctcgatg aactggctga ttatctcgat ttgagcgcct
      421 cagcgcgccg tagcatcgac gaaactgcca cctgttcgaa atgacgcgca aatgggctta
      481 cagggcgatt cgtcagggct ggcccgaatt ctcacagtgg cttgatgccg tgattcagcg
      541 tgtcgaaatg tacaacgcat cgcttcccgt tccgctttca ccg
//
LOCUS       DAOFSV010000361          550 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.371, whole genome shotgun sequence.
ACCESSION   DAOFSV010000361 DAOFSV010000000
VERSION     DAOFSV010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.371"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            1..550
                     /locus_tag="QBH27_004931"
                     /pseudo
     CDS             1..550
                     /locus_tag="QBH27_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          128 a          142 c          157 g          123 t
ORIGIN      
        1 gaaggcgtgg tgcgtaacgg taaaagtact gccggacatc agcgctatct ctgctctcac
       61 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacacaccag
      121 aaaatcattg atatggccat gaatggtgtt ggatgccggg caaccgcccg cattatgggc
      181 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
      241 gcatacagcc gggcagtgat gtgattgtct gcgctgaaat ggacgaacag tggggctacg
      301 tcggtgctaa atcacgtcag cgctggctgt tttacgcgta tgacaggata cggaggacgg
      361 ttgtggcgca cgtcttcggt gaacgcactc tggccacact ggagcgtctt ctgagcctgc
      421 tgtcggcctt tgaggtcgtg gtatggatga cggatggctg gccgctgtat gaatcccgcc
      481 tgaagggaaa gctgcacgta atcagcaagc gttacactca gcgcattgag cgacataacc
      541 tgaatctgag
//
LOCUS       DAOFSV010000362          506 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.372, whole genome shotgun sequence.
ACCESSION   DAOFSV010000362 DAOFSV010000000
VERSION     DAOFSV010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.372"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            194..>506
                     /gene="raiA"
                     /locus_tag="QBH27_004932"
     CDS             194..>506
                     /gene="raiA"
                     /locus_tag="QBH27_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDQ7017109.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DA"
BASE COUNT          179 a          122 c           95 g          110 t
ORIGIN      
        1 agtcactatc gatctcatca aatatggccc gctttgagat attcctcaag taaaaaaaca
       61 cctcttcctg cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga
      121 tttccatcac acattttgac atcaggaacg ttatgctgaa ttcaccaaga cgggaagaca
      181 agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat
      241 ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa
      301 tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac
      361 acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga
      421 attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg
      481 tgccgcaaca tcggtgaaag acgcca
//
LOCUS       DAOFSV010000363          496 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.373, whole genome shotgun sequence.
ACCESSION   DAOFSV010000363 DAOFSV010000000
VERSION     DAOFSV010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.373"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..400)
                     /locus_tag="QBH27_004933"
     CDS             complement(<1..400)
                     /locus_tag="QBH27_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HDQ7017110.1"
                     /translation="MKITAKIIYRLTIALMIAGSVQTAAKEKISLLAPYDEWYFLFFY
                     PNALPAQVTYVELLDTDGIYYQFRTLDATAPSSTTIGQWRDNLRVGVLSFNKAKNPPM
                     SIHFCWDSVIDKKVYETWITLAGEVWKLMLT"
BASE COUNT          138 a           99 c           96 g          163 t
ORIGIN      
        1 gtgtgagcat caatttccag acttctcccg ccagcgttat ccaggtttca tacacttttt
       61 tatcaatcac tgaatcccag cagaaatgga tagacatagg gggatttttg gctttattga
      121 atgataacac cccaacccga agattatctc tccactgacc aatagtagtg gaactcggag
      181 cggtagcatc cagcgttctg aactgataat agataccatc agtgtccaat agttctacat
      241 aggtaacttg tgctggtagc gcattagggt agaaaaaaag gaaataccat tcatcatagg
      301 gtgcaagcag tgatattttt tctttcgctg ctgtctggac tgatccggct atcattaacg
      361 caatggttaa tctataaatt attttagctg ttattttcat tgttacttac cagttaccat
      421 agggatattt tttatctttg ataaagtttt ttgtactttc cggataagaa tacccatgtt
      481 tataggcact tcggcc
//
LOCUS       DAOFSV010000364          464 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.374, whole genome shotgun sequence.
ACCESSION   DAOFSV010000364 DAOFSV010000000
VERSION     DAOFSV010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.374"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>464
                     /gene="vgrG"
                     /locus_tag="QBH27_004934"
     CDS             <1..>464
                     /gene="vgrG"
                     /locus_tag="QBH27_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ7017111.1"
                     /translation="EAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGD
                     DAPAVFRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARV
                     TSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLH"
BASE COUNT           98 a          146 c          142 g           78 t
ORIGIN      
        1 agaggcagcg gaaagcggcg cgttttatgc ccgtatccgc cacgaacgct acctgaatga
       61 acaggccatt ctcaaaggcc agagcaccag ttcactgctg atgccggggc tggaaatcag
      121 ggttcagggc gacgacgcgc cagcagtgtt ccgcaagggc gtactgatta ccggcgtgac
      181 ggccagcgcg gcgcgtgacc gcagctatga gctgacgttt accgccatcc cgtactcaga
      241 gcgctacggc taccgcccgg cgcttatccc ccgcccggta atggccggca cactcccggc
      301 gcgggtcacc agcacggtga aaaacgatat ctatgcccat atcgacaagg acgggcgcta
      361 ccgggtcaac ctcgactttg accgcgatac gtggaaaccg ggttatgaaa gcctgtgggt
      421 gcgccagtcg cgcccgtatg caggtgacac gtatggcctg catc
//
LOCUS       DAOFSV010000365          453 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.375, whole genome shotgun sequence.
ACCESSION   DAOFSV010000365 DAOFSV010000000
VERSION     DAOFSV010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.375"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..449
                     /locus_tag="QBH27_004935"
     CDS             <1..449
                     /locus_tag="QBH27_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7017112.1"
                     /translation="VAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVH
                     HSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNR
                     AEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA"
BASE COUNT          116 a          101 c          135 g          101 t
ORIGIN      
        1 atgtggcgtt tatcattgat gtgtttgctg gatacatcgt ggggtggcgg gtctcatcgt
       61 ctatggaaac gacattcgtg ctggatgcgc tggagcaggc gttgtgggcc cgtcgaccgt
      121 ccggcacggt ccatcacagt gataaaggtt ctcagtatgt atcgctggcc tacacacagc
      181 ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg tatgacaacg
      241 cgatggctga gagcatcaat ggtctttaca aagcggaggt aatacaccgt aagagctgga
      301 aaaaccgtgc agaagtggaa ctggccacac taacgtgggt ggactggtat aacaatcgac
      361 gattgctggg aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt
      421 ccatcggaaa caatgatctg gcagcctgag ttc
//
LOCUS       DAOFSV010000366          447 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.376, whole genome shotgun sequence.
ACCESSION   DAOFSV010000366 DAOFSV010000000
VERSION     DAOFSV010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.376"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            29..301
                     /locus_tag="QBH27_004936"
     CDS             29..301
                     /locus_tag="QBH27_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017113.1"
                     /translation="MDNNTISLQELLDSISRLREDVNTLTVAFSYLAFSIPREQMQST
                     LASIQFESCNPKWSQEQQDSFRRLAVLLDEKYAGKITISADSSENP"
     gene            complement(267..>447)
                     /locus_tag="QBH27_004937"
     CDS             complement(267..>447)
                     /locus_tag="QBH27_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HDQ7017114.1"
                     /translation="AARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQDVLITYRGK
                     LPGIITGSLKSPPKW"
BASE COUNT          118 a          119 c           89 g          121 t
ORIGIN      
        1 caggtcaata caatcatgag gattatttat ggacaataac accatttctc tacaggagtt
       61 gctcgacagc atttccaggc ttcgggaaga cgtgaatacc cttaccgtcg ccttctcata
      121 tctggcattc tcaattccaa gggaacagat gcaatcaacg ctggcatcaa tccagtttga
      181 atcatgcaat cccaaatggt ctcaggaaca acaagactct ttcaggcggc ttgctgtatt
      241 actggatgaa aaatatgctg gtaaaattac catttcggcg gactcttcag agaacccgta
      301 attattcccg gtagttttcc tctgtaggtt atcaacacat cctgcgcctc taaaattacg
      361 gggcgctttt ccggcgactg ctcatcccct tcacataacc cggcagcaac atccaggaag
      421 acctgtctga tgctccttct ggctgct
//
LOCUS       DAOFSV010000367          432 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.377, whole genome shotgun sequence.
ACCESSION   DAOFSV010000367 DAOFSV010000000
VERSION     DAOFSV010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.377"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            5..>432
                     /locus_tag="QBH27_004938"
     CDS             5..>432
                     /locus_tag="QBH27_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDQ7017115.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRG"
BASE COUNT          100 a          123 c          121 g           88 t
ORIGIN      
        1 aattatgcga ctagccagtc gttttggtta tgcgaaccag atacgccgtg accgtccgct
       61 gacacacgaa gaactgatgc actatgtgcc ggggattttc ggggaagata aacacacgtc
      121 ccgaagccag aactatacgt acatccccac catcaccgta ctggaaagcc tgcagcggga
      181 aggctttcag ccattcttcg cctgccagac ccgtgtgcgc gacccgggcc gccggggata
      241 cacaaaacac atgctgcgtc tgcggcgggc cggagagata aacggagaac atgtccctga
      301 aattattctg ctcaactctc atgacggtac ctccagctac cagatgctgc cgggttactt
      361 caggttcgtc tgccagaacg ggtgcgtctg tggtcagtct ctgggggaag tgcgtgttcc
      421 gcaccgggga aa
//
LOCUS       DAOFSV010000368          426 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.378, whole genome shotgun sequence.
ACCESSION   DAOFSV010000368 DAOFSV010000000
VERSION     DAOFSV010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.378"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            227..>426
                     /locus_tag="QBH27_004939"
     CDS             227..>426
                     /locus_tag="QBH27_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDQ7017116.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKA"
BASE COUNT          104 a          104 c          105 g          113 t
ORIGIN      
        1 ttgactgaaa ccatgtatac tctcaggccc cacgcacatg tgttggggcc tttttacatg
       61 caaaaaaaag cccgcatatt gatacgggcg gcaaggaaca aaaaacgtga agtgatctat
      121 tcagccagtg gataataccc tgacgttacc atattgcgca atcgcgcatt ctttcttttt
      181 cgctcccctt ataactacgc catccgttcg ctgcggaggt gaggctatga aatccatgga
      241 taaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
      301 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
      361 ggttctgggc ttcctgacat atctgactaa cctgtatttc aaaatcagag aggaccgtcg
      421 taaggc
//
LOCUS       DAOFSV010000369          425 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.379, whole genome shotgun sequence.
ACCESSION   DAOFSV010000369 DAOFSV010000000
VERSION     DAOFSV010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.379"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            92..>425
                     /locus_tag="QBH27_004940"
     CDS             92..>425
                     /locus_tag="QBH27_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017117.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGE
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTT"
BASE COUNT          129 a           85 c           92 g          119 t
ORIGIN      
        1 ggctggtgtt attgaaaata ttttcagata tatattacct gagagaaagt tttacccctc
       61 acctgtcaat tcactcactg gaaatttaat tatgcaatta cctgtaaagt tattaatgag
      121 ccttatatct ctggtcagcg ttattgcacg tgccgggaaa tataaaaatt acatccggga
      181 tgaaataaaa tactggcgat atacatcata caaggggggg gaatttccgg aaggtttcac
      241 tgatgagaaa ttttccagcg ccatttacaa cggaagaata tttacaatga aacgtttaca
      301 taccctgatg ttatttctgg cggttctgtt tactggcttt aacgtggaag cagcgagcgt
      361 gaaacaagcg ctcagctgcg acccaaacgc ccgggctgaa caacctggag cgtgtccaac
      421 aacgt
//
LOCUS       DAOFSV010000370          422 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.380, whole genome shotgun sequence.
ACCESSION   DAOFSV010000370 DAOFSV010000000
VERSION     DAOFSV010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.380"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            11..346
                     /gene="iraM"
                     /locus_tag="QBH27_004941"
     CDS             11..346
                     /gene="iraM"
                     /locus_tag="QBH27_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDQ7017118.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          151 a           85 c           73 g          113 t
ORIGIN      
        1 aggaaactaa atgaagtgga ttgtgattga tacagttatc cagccatcat gcggaatatc
       61 tttttcagtc atatggagta aaataaaatt aataatctgg tatcaatcgg atgctttctt
      121 acctcctgaa agtatattta cactgactca cacaggcatc atgctcaata acaaagtgct
      181 acctgtaacc atttacaacg tagtaccatt caataaaaca ttctggaatt taatcaaaaa
      241 cagccaggaa tgccctacaa atacagataa cgtattgaat gaatgcttta ataaccgttg
      301 cactctgcaa atatgtcctt atggactaaa acaacaaagt ccataaggag tttactcaca
      361 tctgacaaaa tcaatataaa cagcccctcc ggagaggggc tggagagtgg cgctatgtgc
      421 ca
//
LOCUS       DAOFSV010000371          416 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.381, whole genome shotgun sequence.
ACCESSION   DAOFSV010000371 DAOFSV010000000
VERSION     DAOFSV010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.381"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            43..>416
                     /locus_tag="QBH27_004942"
     CDS             43..>416
                     /locus_tag="QBH27_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDQ7017119.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DAOFSV010000372          405 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.382, whole genome shotgun sequence.
ACCESSION   DAOFSV010000372 DAOFSV010000000
VERSION     DAOFSV010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.382"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
BASE COUNT          127 a           71 c           78 g          129 t
ORIGIN      
        1 tagttttgag acatctctat gagtctcagg acagttaaaa aataaaaatt atttttaccc
       61 ggtcactttg taaaaaagtg gccgggtttt tgatttctgt gtttcgtgcg cttaatcgag
      121 tctcacgctt cagtgagcaa attgcccctc cccaacagac atcacgattc acaaggtcaa
      181 aaattctgtt ccatgaagta ccgtacaaag ataaagagaa aaatgcctta tgcgggatgt
      241 agctgtcgcg tctttatcgg gaaactatac gtaattgcca ctttgtcttc ggtaatcaga
      301 aaaagaagac ctgccatttt aataaatatt atttctctgg gaacggggat tttttataag
      361 caatatgacg agttaaacat tatattttta taaggattat atgag
//
LOCUS       DAOFSV010000373          393 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.383, whole genome shotgun sequence.
ACCESSION   DAOFSV010000373 DAOFSV010000000
VERSION     DAOFSV010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.383"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..183
                     /locus_tag="QBH27_004943"
     CDS             <1..183
                     /locus_tag="QBH27_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDQ7017120.1"
                     /translation="DGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVK
                     TKVKGTVVMFSASGKK"
     gene            complement(238..>393)
                     /locus_tag="QBH27_004944"
     CDS             complement(238..>393)
                     /locus_tag="QBH27_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ7017121.1"
                     /translation="EFQKWNGTSWIKDTEEETSLLEAWKMYRVLLNRVDTSTAPDIEW
                     PVNPVRE"
BASE COUNT          114 a           99 c           79 g          101 t
ORIGIN      
        1 gacggcgttg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc
       61 atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc
      121 gagaatgtta aaaccaaggt taaaggtact gtagtaatgt tttccgcttc agggaaaaaa
      181 tagcatccag acgcagcacg ttcttgcgca cgacgtgctg cggcataatc ccaatgatta
      241 ctctctgaca gggttcacag gccactcaat atcaggggca gttgatgtat caactcggtt
      301 cagcaacacc cgatacatct tccaggcttc cagcaacgag gtttcttcct ccgtatcctt
      361 tatccatgat gtgccgttcc acttctgaaa ctc
//
LOCUS       DAOFSV010000374          384 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.384, whole genome shotgun sequence.
ACCESSION   DAOFSV010000374 DAOFSV010000000
VERSION     DAOFSV010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.384"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            48..124
                     /locus_tag="QBH27_004945"
     tRNA            48..124
                     /locus_tag="QBH27_004945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="QBH27_004946"
     tRNA            167..242
                     /locus_tag="QBH27_004946"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
BASE COUNT          107 a           83 c           92 g          102 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catctctgta gtgattaaat aaaaatactt cagagtgtac ctgcaaaggt tcactgcgaa
      301 gttttgctct ttaaaaatct ggatcaagct gaaaattgaa acactgaaca acgaaagttg
      361 ttcgtgagtc tctcaaattt tcgc
//
LOCUS       DAOFSV010000375          383 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.385, whole genome shotgun sequence.
ACCESSION   DAOFSV010000375 DAOFSV010000000
VERSION     DAOFSV010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.385"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..372
                     /locus_tag="QBH27_004947"
     CDS             <1..372
                     /locus_tag="QBH27_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ7017122.1"
                     /translation="RPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALA
                     DRGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKR
                     IKLSLGGLSPVEYRTEYQKAG"
BASE COUNT          125 a           74 c          107 g           77 t
ORIGIN      
        1 aggcctacag aggagctggt gaagcggatg ttaaataagg gtctggaaag ccttgctgaa
       61 ggggagaaac cgctgctcca cagtgatcaa ggatggcatt accggataaa aagttatcag
      121 tccgccctgg cggacagagg gttagtgcag agcatgtcgc gcaagggcaa ctgccttgat
      181 aatgcggtaa tggagaactt ctttggtcac ctgaaagaag aaatgtacta tcgccgggac
      241 tacagaaatg tagaagagct ggaaaatgcc gttaacgaat acataactta ctggaatcaa
      301 aaaagaataa aactcagtct tgggggactc agcccggtag aataccggac tgagtatcaa
      361 aaagccggtt aactgaaact gtc
//
LOCUS       DAOFSV010000376          354 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.386, whole genome shotgun sequence.
ACCESSION   DAOFSV010000376 DAOFSV010000000
VERSION     DAOFSV010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.386"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>354
                     /locus_tag="QBH27_004948"
     CDS             <1..>354
                     /locus_tag="QBH27_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDQ7017123.1"
                     /translation="SAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPGSDDLNGINV
                     KYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWF
                     SAYAMAGVAYSRVST"
BASE COUNT           73 a           88 c          113 g           80 t
ORIGIN      
        1 tgtccgcagt agtctggcag gtcgccgctg ctacgccagc gagtgcagca gaacatcagt
       61 ccacgctgag cgcggggtat ctccatgcct cgacgaacgt tcccggtagt gatgatctga
      121 acgggattaa cgtgaaatac cgttatgagt ttacggacgc gctggggctg attacgtcct
      181 tcagttatgc caatgctgag gatgagcaaa aaacgcgcta cagcgatacc cgctggcatg
      241 aagattccgt gcgtaaccgc tggttcagcg tgatggcggg gccgtctgta cgcgtgaatg
      301 aatggttcag cgcgtatgcg atggcgggtg tggcttacag ccgtgtgtcg actt
//
LOCUS       DAOFSV010000377          349 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.387, whole genome shotgun sequence.
ACCESSION   DAOFSV010000377 DAOFSV010000000
VERSION     DAOFSV010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.387"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            18..>349
                     /locus_tag="QBH27_004949"
     CDS             18..>349
                     /locus_tag="QBH27_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDQ7017124.1"
                     /translation="MVAAGTPASAAEHQSTLSAGYLQTHTDMPGSDNLNGINVKYRYE
                     FTDTLGLITSFSYADAEDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAM
                     AGVAYSRVS"
BASE COUNT           82 a           85 c          104 g           78 t
ORIGIN      
        1 tgtccgcagt agtctggctg gttgccgctg gtacgccagc gagcgcagca gagcatcagt
       61 ccacactaag cgccgggtat cttcagaccc acactgatat gccaggaagc gataatctga
      121 acgggattaa cgtgaaatac cgttatgaat ttacggacac gctggggctg attacgtctt
      181 tcagttatgc cgacgcagaa gatgaacaaa aaacgcatta cagcgatacc cgctggcatg
      241 aagattccgt gcgtaaccgc tggttcagcg taatggcggg gccgtctgtg cgcgtgaatg
      301 aatggttcag cgcgtatgcg atggctggtg tggcttacag ccgtgtgtc
//
LOCUS       DAOFSV010000378          344 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.388, whole genome shotgun sequence.
ACCESSION   DAOFSV010000378 DAOFSV010000000
VERSION     DAOFSV010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.388"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>344
                     /locus_tag="QBH27_004950"
     CDS             <1..>344
                     /locus_tag="QBH27_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ7017125.1"
                     /translation="GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVE
                     LREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADT
                     GIDIDMHIDAASG"
BASE COUNT           80 a           97 c          103 g           64 t
ORIGIN      
        1 ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagccgcagg caaacccacg
       61 gataaaccaa acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac
      121 tgggatgtgg agttgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa
      181 cgcatgattg aagcctgcga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc
      241 tacaccggta actatgagtt cccgcagccg ctgcacgatg cgctggataa attccaggcc
      301 gacaccggta tcgacatcga catgcacatc gacgccgcca gcgg
//
LOCUS       DAOFSV010000379          334 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.389, whole genome shotgun sequence.
ACCESSION   DAOFSV010000379 DAOFSV010000000
VERSION     DAOFSV010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.389"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
BASE COUNT          101 a           68 c           65 g          100 t
ORIGIN      
        1 agtaacatcc catgaacacg atcaggattg ttggtatcga tattgccagg cctgtttttc
       61 aggcctatat ctggattaat gatgattctg ttgcatagga tagaaacacc tcatgctcca
      121 aattgcagga tatcgttcaa cgattcgagt ccggaacact attcgctatg gaggcatgtt
      181 caacttctca ttctggggga tgaacgttca gttctatgga atatcccctg actattccct
      241 gcacaacagc atcatttttg gtaaaaaaaa taacattatt cttaatatat agaaaaggaa
      301 ctcgtcctgg acgggtacaa cttattaaaa atgc
//
LOCUS       DAOFSV010000380          315 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.390, whole genome shotgun sequence.
ACCESSION   DAOFSV010000380 DAOFSV010000000
VERSION     DAOFSV010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.390"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>315
                     /locus_tag="QBH27_004951"
     CDS             <1..>315
                     /locus_tag="QBH27_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7017126.1"
                     /translation="KTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYA
                     DDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESY
                     LRY"
BASE COUNT           83 a           78 c           97 g           57 t
ORIGIN      
        1 aaaacgaaac cgctgctgaa atccctggaa agctggctgc gtgaaaagat gaaaaccctg
       61 tcgcgacact cagaactggc gaaagcgttc gcatacgccc tgaaccagtg gccggcgctg
      121 acgtactatg cagatgatgg ctgggctgag gcggacaata acatcgctga aaatgcgttg
      181 cggatggtca gtctgggccg caaaaactac ctgttcttcg gttcggatca tggaggagag
      241 cggggagcgc tgctgtacag cctgatcggg acgtgcaaac tgaacggagt ggagccagaa
      301 agctacctcc gctat
//
LOCUS       DAOFSV010000381          303 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.391, whole genome shotgun sequence.
ACCESSION   DAOFSV010000381 DAOFSV010000000
VERSION     DAOFSV010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.391"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>303
                     /gene="istB"
                     /locus_tag="QBH27_004952"
     CDS             <1..>303
                     /gene="istB"
                     /locus_tag="QBH27_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDQ7017127.1"
                     /translation="ESLISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMY
                     TRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLA"
BASE COUNT           88 a           77 c           77 g           61 t
ORIGIN      
        1 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
       61 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
      121 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
      181 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
      241 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
      301 ggc
//
LOCUS       DAOFSV010000382          300 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.392, whole genome shotgun sequence.
ACCESSION   DAOFSV010000382 DAOFSV010000000
VERSION     DAOFSV010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.392"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
BASE COUNT           93 a           64 c           52 g           91 t
ORIGIN      
        1 tctcggatta atgcagtcag taaaagtatg gcactgactg cattaagaag ggacgtccat
       61 catatcgtga cacaaacgct cccaccgaca tcgccattga gtcctgcacc gtatcggtga
      121 acttgtaact atcccgtata atcattctat acacatttat tggcttgatg agtcaaatac
      181 ttcttaattt cggagagaag tgtgtcaggt gatacgttaa ctcgccatta tctacatatt
      241 tagagaatta ctcaaataat aacgaattat ttacccatgt gctcaattaa tcttcgaata
//
LOCUS       DAOFSV010000383          299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.393, whole genome shotgun sequence.
ACCESSION   DAOFSV010000383 DAOFSV010000000
VERSION     DAOFSV010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.393"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>299
                     /locus_tag="QBH27_004953"
     CDS             <1..>299
                     /locus_tag="QBH27_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HDQ7017128.1"
                     /translation="YFNFFYPNSLPAQVTYVELLDTDGIYYQFRTLDSTIPVSTTIGQ
                     WQKNLSGGMTAFNKAKNPPMSIHFCWDSVIDKKEYETWITLAGDVWKLMLTPYSPP"
BASE COUNT           94 a           66 c           54 g           85 t
ORIGIN      
        1 tatttcaatt ttttttaccc taattcccta ccagcacaag ttacctatgt ggaattatta
       61 gataccgatg gcatctatta tcagttcaga actctggata gcactatccc tgtctcaaca
      121 acaataggac aatggcagaa aaatcttagt ggtggaatga cggcattcaa taaagccaaa
      181 aatcccccta tgtctatcca tttctgctgg gattcagtga ttgataaaaa agagtatgaa
      241 acctggataa cgctggcggg agacgtctgg aaattgatgc tcacacctta ttcaccacc
//
LOCUS       DAOFSV010000384          287 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.394, whole genome shotgun sequence.
ACCESSION   DAOFSV010000384 DAOFSV010000000
VERSION     DAOFSV010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.394"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            84..242
                     /locus_tag="QBH27_004954"
     CDS             84..242
                     /locus_tag="QBH27_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDQ7017129.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT           67 a           73 c           77 g           70 t
ORIGIN      
        1 tcattaacca ccacgaggca tccctatgtc tagtccacat caggatagcc tcttaccgcg
       61 ctttgcgcaa ggagaagaag gccatgaaac taccacgaag ttcccttgtc tggtgtgtgt
      121 tgatcgtgtg tctcacactg ttgatattca cttatctgac acgaaaatcg ctgtgcgaga
      181 ttcgttacag agacggacac agggaggtgg cggctttcat ggcttacgaa tccggtaagt
      241 agcaacctgg aggcgggcgc aggcccgcct tttcaggact gatgctg
//
LOCUS       DAOFSV010000385          282 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.396, whole genome shotgun sequence.
ACCESSION   DAOFSV010000385 DAOFSV010000000
VERSION     DAOFSV010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.396"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
BASE COUNT          106 a           58 c           29 g           89 t
ORIGIN      
        1 actgtccata tcataaataa gttacgtatt tttctcaagc ataaaaatat taaaaaacga
       61 caaaaagcat ctaactgttt gatatgtaaa ttatttctat tgtaaattaa tttcacatca
      121 cctccgctat atgtaaagct aacgtttctg tggctcgacg catcttcctc attcttctct
      181 ccaaaaacca cctcatgcaa tataaacatc tataaataaa gataacaata gaatattaag
      241 ccaacaaata aactgaaaaa gtttgtccgc gatgctttcc tc
//
LOCUS       DAOFSV010000386          266 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.397, whole genome shotgun sequence.
ACCESSION   DAOFSV010000386 DAOFSV010000000
VERSION     DAOFSV010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.397"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            85..>266
                     /locus_tag="QBH27_004955"
     CDS             85..>266
                     /locus_tag="QBH27_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDQ7017130.1"
                     /translation="MYNFKDKIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDL"
BASE COUNT           97 a           37 c           59 g           73 t
ORIGIN      
        1 tatagtggcg gaatttacga tattgataat ttaaggaggg cacacctcat ttgcatgatg
       61 aaatttatta caggagatag ttaaatgtat aattttaaag ataaaataga agattacaca
      121 gagcgggaat tcattgagtt gcttggcgag tttacaaatc caaccgggga taacgctcaa
      181 ttaaaagggg aagagcttga caaatattgg gatgatctag aagaacatct taccagaatt
      241 acacaacatc cgttgatgag cgatct
//
LOCUS       DAOFSV010000387          263 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.398, whole genome shotgun sequence.
ACCESSION   DAOFSV010000387 DAOFSV010000000
VERSION     DAOFSV010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.398"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            65..140
                     /locus_tag="QBH27_004956"
     tRNA            65..140
                     /locus_tag="QBH27_004956"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Glu,seq:ttc)
BASE COUNT           74 a           61 c           63 g           65 t
ORIGIN      
        1 agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg aagcagactg
       61 atacgtcccc ttcgtctaga ggcccaggac accgcccttt cacggcggta acaggggttc
      121 gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcacctgc cttaatatct
      181 caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg gatcaagctg
      241 aaaattgaaa cactgaacaa cga
//
LOCUS       DAOFSV010000388          255 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.399, whole genome shotgun sequence.
ACCESSION   DAOFSV010000388 DAOFSV010000000
VERSION     DAOFSV010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.399"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
BASE COUNT           72 a           66 c           64 g           53 t
ORIGIN      
        1 tttttcgctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
       61 ggcaaacacg ccgccgggtg agcggggttc tcctgagaat tccggcagag aaagcaaaaa
      121 taaatgcttg actctgtagc gggaaagcgt attatgcaca ccccgcgccg ctgagaaaaa
      181 gcgaagcggc actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac
      241 ggattcttaa cgtcg
//
LOCUS       DAOFSV010000389          236 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.400, whole genome shotgun sequence.
ACCESSION   DAOFSV010000389 DAOFSV010000000
VERSION     DAOFSV010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.400"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(1..235)
                     /locus_tag="QBH27_004957"
                     /pseudo
     CDS             complement(1..235)
                     /locus_tag="QBH27_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024169763.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
BASE COUNT           72 a           63 c           44 g           57 t
ORIGIN      
        1 gcaagccttg ctgcaagagc attcagccgt ataagtctcc ccagtgttgt actgaaatac
       61 ccattattca tctggtattt ctcgcagacc tccttacggg agtgcccacc taccagatag
      121 tccttcatag ccagaatgac cctgtcactg tgaatagaag aaatacctat cagtaaaaaa
      181 aatgcatttc agacatagag ccgagcaaca gcacgctctc gcgtatattc agcaaa
//
LOCUS       DAOFSV010000390          233 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.401, whole genome shotgun sequence.
ACCESSION   DAOFSV010000390 DAOFSV010000000
VERSION     DAOFSV010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.401"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>233
                     /locus_tag="QBH27_004958"
     CDS             <1..>233
                     /locus_tag="QBH27_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7017131.1"
                     /translation="RSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLAR
                     VLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG"
BASE COUNT           42 a           72 c           76 g           43 t
ORIGIN      
        1 gcgtagcgcc ttccgggtta tccggacggt acgggaaaaa catgcctgta ctcagtgcga
       61 tgccatcgtg caggcacctg caccttcgcg gcccatcgag cggggtatcg ccggaccggg
      121 gctgctggcc cgcgtgctga cctcgaagta tgcagagcac accccgctgt atcgccagtc
      181 agaaatatac ggccggcaag gtgtggagct gagccgttca ctgctgtcgg gct
//
LOCUS       DAOFSV010000391          232 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.402, whole genome shotgun sequence.
ACCESSION   DAOFSV010000391 DAOFSV010000000
VERSION     DAOFSV010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.402"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>232
                     /gene="ihfB"
                     /locus_tag="QBH27_004959"
     CDS             <1..>232
                     /gene="ihfB"
                     /locus_tag="QBH27_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDQ7017132.1"
                     /translation="ATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIEIRGFGSFSLH
                     YRAPRTGRNPKTGDKVELEGKYVPHFKPGKELR"
BASE COUNT           65 a           60 c           59 g           48 t
ORIGIN      
        1 ttgccaccca gcaatcgcac attcccgcca agacggttga agatgcagta aaagagatgc
       61 tggagcatat ggcctcgact cttgcgcagg gcgagcgtat tgaaatccgc ggtttcggca
      121 gtttctcttt gcactaccgc gcaccacgta ccggacgtaa tccgaagact ggcgataaag
      181 tagaactgga aggaaaatac gttcctcact ttaaaccagg taaagaactg cg
//
LOCUS       DAOFSV010000392          211 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.denovo.403, whole genome shotgun sequence.
ACCESSION   DAOFSV010000392 DAOFSV010000000
VERSION     DAOFSV010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.denovo.403"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            <1..>211
                     /locus_tag="QBH27_004960"
     CDS             <1..>211
                     /locus_tag="QBH27_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDQ7017133.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFIC"
BASE COUNT           66 a           57 c           36 g           52 t
ORIGIN      
        1 gaattacact taaaacgact caacagctta acgttggctt gccacgcatt acttgactgt
       61 aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag aacaaaacat
      121 aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga gcgactcgct
      181 gtataccgtt ggcatgctag ctttatctgt t
//
LOCUS       DAOFSV010000393         2824 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.280, whole genome shotgun sequence.
ACCESSION   DAOFSV010000393 DAOFSV010000000
VERSION     DAOFSV010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.280"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            308..1123
                     /gene="sul2"
                     /locus_tag="QBH27_004961"
     CDS             308..1123
                     /gene="sul2"
                     /locus_tag="QBH27_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDQ7017134.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1184..1987
                     /locus_tag="QBH27_004962"
                     /note="aph(3'')-Ib"
     CDS             1184..1987
                     /locus_tag="QBH27_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDQ7017135.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1987..2823
                     /locus_tag="QBH27_004963"
     CDS             1987..2823
                     /locus_tag="QBH27_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDQ7017136.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          568 a          782 c          793 g          681 t
ORIGIN      
        1 ccccccccac tctattgtaa acaagacatt tttatctttt atattcaatg gcttattttc
       61 ctgctaattg gtaataccat gaaaaatacc atgctcagaa aaggcttaac aatattttga
      121 aaaattgcct actgagcgct gccgcacagc tccataggcc gctttcctgg ctttgcttcc
      181 agatgtatgc tcttctgctc ctgcagctaa tggatcaccg caaacaggtt actcgcctgg
      241 ggattccctt tcgacccgag catccgtatg atactcatgc tcgattatta ttattataga
      301 agcccccatg aataaatcgc tcatcatttt cggcatcgtc aacataacct cggacagttt
      361 ctccgatgga ggccggtatc tggcgccaga cgcagccatt gcgcaggcgc gtaagctgat
      421 ggccgagggg gcagatgtga tcgacctcgg tccggcatcc agcaatcccg acgccgcgcc
      481 tgtttcgtcc gacacagaaa tcgcgcgtat cgcgccggtg ctggacgcgc tcaaggcaga
      541 tggcattccc gtctcgctcg acagttatca acccgcgacg caagcctatg ccttgtcgcg
      601 tggtgtggcc tatctcaatg atattcgcgg ttttccagac gctgcgttct atccgcaatt
      661 ggcgaaatca tctgccaaac tcgtcgttat gcattcggtg caagacgggc aggcagatcg
      721 gcgcgaggca cccgctggcg acatcatgga tcacattgcg gcgttctttg acgcgcgcat
      781 cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt gtccttgatc ccggcatggg
      841 gttttttctg ggggctgctc ccgaaacctc gctctcggtg ctggcgcggt tcgatgaatt
      901 gcggctgcgc ttcgatttgc cggtgcttct gtctgtttcg cgcaaatcct ttctgcgcgc
      961 gctcacaggc cgtggtccgg gggatgtcgg ggccgcgaca ctcgctgcag agcttgccgc
     1021 cgccgcaggt ggagctgact tcatccgcac acacgagccg cgccccttgc gcgacgggct
     1081 ggcggtattg gcggcgctga aagaaaccgc aagaattcgt taactgcaca ttcgggatat
     1141 ttctctatat tcgcgcttca tcagaaaact gaaggaacct ccattgaatc gaactaatat
     1201 tttttttggt gaatcgcatt ctgactggtt gcctgtcaga ggcggagaat ctggtgattt
     1261 tgtttttcga cgtggtgacg ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg
     1321 tgagctcgct ggagagcgtg accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc
     1381 cgaggtcatc aactggcagg aggaacagga gggtgcatgc ttggtgataa cggcaattcc
     1441 gggagtaccg gcggctgatc tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg
     1501 gcagcaactt ggcgctgttc acagcctatc ggttgatcaa tgtccgtttg agcgcaggct
     1561 gtcgcgaatg ttcggacgcg ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt
     1621 cttaccggac gaggacaaga gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga
     1681 gctaccggtg cggctcgacc aagagcgcac cgatatggtt gtttgccatg gtgatccctg
     1741 catgccgaac ttcatggtgg accctaaaac tcttcaatgc acgggtctga tcgaccttgg
     1801 gcggctcgga acagcagatc gctatgccga tttggcactc atgattgcta acgccgaaga
     1861 gaactgggca gcgccagatg aagcagagcg cgccttcgct gtcctattca atgtattggg
     1921 gatcgaagcc cccgaccgcg aacgccttgc cttctatctg cgattggacc ctctgacttg
     1981 gggttgatgt tcatgccgcc tgtttttcct gctcattggc acgtttcgca acctgttctc
     2041 attgcggaca ccttttccag cctcgtttgg aaagtttcat tgccagacgg gactcctgca
     2101 atcgtcaagg gattgaaacc tataaaagac attgctgatg aactgcgcgg ggccgactat
     2161 ctggtatggc gcaatgggag gggagcagtc cggttgctcg gtcgtgagaa caatctgatg
     2221 ttgctcgaat atgccgggga gcgaatgctc tctcacatcg ttgccgagca cggcgactac
     2281 caggcgaccg aaattgcagc ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc
     2341 ctgccttctg cccttctccc gatccgggat cgctttgcag ctttgtttca gcgggcgcgc
     2401 gatgatcaaa acgcaggttg tcaaactgac tacgtccacg cggcgattat agccgatcaa
     2461 atgatgagca atgcctcgga actgcgtggg ctacatggcg atctgcatca tgaaaacatc
     2521 atgttctcca gtcgcggctg gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc
     2581 tttggcgccg ccaatatgtt ctacgatccg gctgacagag acgacctttg tctcgatcct
     2641 agacgcattg cacagatggc ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc
     2701 ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag cttggaacgc ggatggagaa
     2761 gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac
     2821 taga
//
LOCUS       DAOFSV010000394         1729 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.316, whole genome shotgun sequence.
ACCESSION   DAOFSV010000394 DAOFSV010000000
VERSION     DAOFSV010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.316"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            7..354
                     /locus_tag="QBH27_004964"
     CDS             7..354
                     /locus_tag="QBH27_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDQ7017137.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            348..1187
                     /gene="sul1"
                     /locus_tag="QBH27_004965"
     CDS             348..1187
                     /gene="sul1"
                     /locus_tag="QBH27_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDQ7017138.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1315..1518
                     /locus_tag="QBH27_004966"
     CDS             1315..1518
                     /locus_tag="QBH27_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ7017139.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
BASE COUNT          341 a          488 c          498 g          402 t
ORIGIN      
        1 tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca
       61 tccgcattaa aatctagcga gggctttact aagcttgccc cttccgccgt tgtcataatc
      121 ggttatggca tcgcatttta ttttctttct ctggttctga aatccatccc tgtcggtgtt
      181 gcttatgcag tctggtcggg actcggcgtc gtcataatta cagccattgc ctggttgctt
      241 catgggcaaa agcttgatgc gtggggcttt gtaggtatgg ggctcataat tgctgccttt
      301 ttgctcgccc gatccccatc gtggaagtcg ctgcggaggc cgacgccatg gtgacggtgt
      361 tcggcattct gaatctcacc gaggactcct tcttcgatga gagccggcgg ctagaccccg
      421 ccggcgctgt caccgcggcg atcgaaatgc tgcgagtcgg atcagacgtc gtggatgtcg
      481 gaccggccgc cagccatccg gacgcgaggc ctgtatcgcc ggccgatgag atcagacgta
      541 ttgcgccgct cttagacgcc ctgtccgatc agatgcaccg tgtttcaatc gacagcttcc
      601 aaccggaaac ccagcgctat gcgctcaagc gcggcgtggg ctacctgaac gatatccaag
      661 gatttcctga ccctgcgctc tatcccgata ttgctgaggc ggactgcagg ctggtggtta
      721 tgcactcagc gcagcgggat ggcatcgcca cccgcaccgg tcaccttcga cccgaagacg
      781 cgctcgacga gattgtgcgg ttcttcgagg cgcgggtttc cgccttgcga cggagcgggg
      841 tcgctgccga ccggctcatc ctcgatccgg ggatgggatt tttcttgagc cccgcaccgg
      901 aaacatcgct gcacgtgctg tcgaaccttc aaaagctgaa gtcggcgttg gggcttccgc
      961 tattggtctc ggtgtcgcgg aaatccttct tgggcgccac cgttggcctt cctgtaaagg
     1021 atctgggtcc agcgagcctt gcggcggaac ttcacgcgat cggcaatggc gctgactacg
     1081 tccgcaccca cgcgcctgga gatctgcgaa gcgcaatcac cttctcggaa accctcgcga
     1141 aatttcgcag tcgcgacgcc agagaccgag ggttagatca tgcctagcat tcaccttccg
     1201 gccgcccgct agcggaccct ggtcaggttc cgcgaaggtg ggcgcagaca tgctgggctc
     1261 gtcaggatca aactgcacta tgaggcggcg gttcataccg cgccagggga gcgaatggac
     1321 agcgaggagc ctccgaacgt tcgggtcgcc tgctcgggtg atatcgacga ggttgtgcgg
     1381 ctgatgcacg acgctgcggc gtggatgtcc gccaagggaa cgcccgcctg ggacgtcgcg
     1441 cggatcgacc ggacattcgc ggagaccttc gtcctgagat ccgagctcct agggatcgcc
     1501 tcagaaaacg gaaaataaag cacgctaagc cgtaagtaag cgtgctcctg tgaaagccac
     1561 agctaaaact gcgtagtaca catagaggta tttttttcta gatttatatc gaatttcgtt
     1621 atcgaatttc gcttattatt gatcaactcg ttgcagatca tgaaggcgaa agtatgacga
     1681 ataaccctac cgatgacagc agaccatggt cggtctttct tatttttct
//
LOCUS       DAOFSV010000395         1650 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.321, whole genome shotgun sequence.
ACCESSION   DAOFSV010000395 DAOFSV010000000
VERSION     DAOFSV010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.321"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            89..1288
                     /gene="tet(A)"
                     /locus_tag="QBH27_004967"
     CDS             89..1288
                     /gene="tet(A)"
                     /locus_tag="QBH27_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDQ7017140.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1320..>1650)
                     /locus_tag="QBH27_004968"
     CDS             complement(1320..>1650)
                     /locus_tag="QBH27_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDQ7017141.1"
                     /translation="LLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFRGIS
                     RLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRAR
                     IACRKSP"
BASE COUNT          291 a          501 c          514 g          344 t
ORIGIN      
        1 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcgga
       61 ccagcggagg ctggtccgga ggccagacgt gaaacccaac agacccctga tcgtaattct
      121 gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct
      181 cctgcgcgat ctggttcact cgaacgacgt caccgcccac tatggcattc tgctggcgct
      241 gtatgcgttg atgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg
      301 gcggcggccg gtcttgctcg tctcgctggc cggcgctgct gtcgactacg ccatcatggc
      361 gacggcgcct ttcctttggg ttctctatat cgggcggatc gtggccggca tcaccggggc
      421 gactggggcg gtagccggcg cttatattgc cgatatcact gatggcgatg agcgcgcgcg
      481 gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg
      541 tgggctgatg ggcggtttct ccccccacgc tccgttcttc gccgcggcag ccttgaacgg
      601 cctcaatttc ctgacgggct gtttcctttt gccggagtcg cacaaaggcg aacgccggcc
      661 gttacgccgg gaggctctca acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt
      721 cgtcgccgcc ctgatggcgg tcttcttcat catgcaactt gtcggacagg tgccggccgc
      781 gctttgggtc attttcggcg aggatcgctt tcactgggac gcgaccacga tcggcatttc
      841 gcttgccgca tttggcattc tgcattcact cgcccaggca atgatcaccg gccctgtagc
      901 cgcccggctc ggcgaaaggc gggcactcat gctcggaatg attgccgacg gcacaggcta
      961 catcctgctt gccttcgcga cacggggatg gatggcgttc ccgatcatgg tcctgcttgc
     1021 ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg tggatgagga
     1081 acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct cgatcgtcgg
     1141 acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg ggtgggcatg
     1201 gattgcaggc gctgccctct acttgctctg cctgccggcg ctgcgtcgcg ggctttggag
     1261 cggcgcaggg caacgagccg atcgctgatc gtggaaacga taggcctatg ccatgcgggt
     1321 caaggcgact tccggcaagc tatacgcgcc ctaggagtgc ggttggaacg ttggcccagc
     1381 cagatactcc cgatcacgag caggacgccg atgatttgaa gcgcactcag cgtctgatcc
     1441 aagaacaacc atcctagcaa cacggcggtc cccgggctga gaaagcccag taaggaaaca
     1501 actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa acccgctccg
     1561 atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg gattggcgga
     1621 tcaaacacta aagctactgg aacgagcaga
//
LOCUS       DAOFSV010000396         1584 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.323, whole genome shotgun sequence.
ACCESSION   DAOFSV010000396 DAOFSV010000000
VERSION     DAOFSV010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.323"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            109..1014
                     /locus_tag="QBH27_004969"
     CDS             109..1014
                     /locus_tag="QBH27_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDQ7017142.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..>1584
                     /locus_tag="QBH27_004970"
     CDS             1011..>1584
                     /locus_tag="QBH27_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ7017143.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPA"
BASE COUNT          244 a          514 c          524 g          302 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgc
//
LOCUS       DAOFSV010000397         1441 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.333, whole genome shotgun sequence.
ACCESSION   DAOFSV010000397 DAOFSV010000000
VERSION     DAOFSV010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.333"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            288..1163
                     /locus_tag="QBH27_004971"
     CDS             288..1163
                     /locus_tag="QBH27_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HDQ7017144.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1198..>1441
                     /locus_tag="QBH27_004972"
     CDS             1198..>1441
                     /locus_tag="QBH27_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ7017145.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVF"
BASE COUNT          369 a          354 c          405 g          313 t
ORIGIN      
        1 gataagttta tcaccaccga ctatttgcaa cagtgccata agaatatcat caataaaatt
       61 gagtgttgct ctgtggataa cttgcagagt ttattaagta tcattgcagc aaagatgaaa
      121 tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa tgtgtgagaa
      181 gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta ataaaaaaca cacgtggaat
      241 ttagggaata ctgatgtaac acggattgac cgtattggga gtttgagatg gtgacaaaga
      301 gagtgcaacg gatgatgttc gcggcggcgg cgtgcattcc gctgctgctg ggcagcgcgc
      361 cgctttatgc gcagacgagt gcggtgcagc aaaagctggc ggcgctggag aaaagcagcg
      421 gagggcggct gggcgtcgcg ctcatcgata ccgcagataa tacgcaggtg ctttatcgcg
      481 gtgatgaacg ctttccaatg tgcagtacca gtaaagttat ggcggccgcg gcggtgctta
      541 agcagagtga aacgcaaaag cagctgctta atcagcctgt cgagatcaag cctgccgatc
      601 tggttaacta caatccgatt gccgaaaaac acgtcaacgg cacaatgacg ctggcagaac
      661 tgagcgcggc cgcgttgcag tacagcgaca ataccgccat gaacaaattg attgcccagc
      721 tcggtggccc gggaggcgtg acggcttttg cccgcgcgat cggcgatgag acgtttcgtc
      781 tggatcgcac tgaacctacg ctgaataccg ccattcccgg cgacccgaga gacaccacca
      841 cgccgcgggc gatggcgcag acgttgcgtc agcttacgct gggtcatgcg ctgggcgaaa
      901 cccagcgggc gcagttggtg acgtggctca aaggcaatac gaccggcgca gccagcattc
      961 gggccggctt accgacgtcg tggactgtgg gtgataagac cggcagcggc ggctacggca
     1021 ccaccaatga tattgcggtg atctggccgc agggtcgtgc gccgctggtt ctggtgacct
     1081 attttaccca gccgcaacag aacgcagaga gccgccgcga tgtgctggct tcagcggcga
     1141 gaatcatcgc cgaagggctg taactggttt tgttgaataa atcgaacttt tgctgagttg
     1201 aaggatcaga tcacgtatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     1261 aaaatcacca actggcccac ctacaataaa gccctcatca accgtggctc cataactttc
     1321 tggctggatg atgaagctat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc
     1381 agacctcagc gctattctga ccttgccatc acgactgtgc tggtcattaa acgcgtattc
     1441 a
//
LOCUS       DAOFSV010000398         1252 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.341, whole genome shotgun sequence.
ACCESSION   DAOFSV010000398 DAOFSV010000000
VERSION     DAOFSV010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.341"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            40..1173
                     /locus_tag="QBH27_004973"
     CDS             40..1173
                     /locus_tag="QBH27_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDQ7017146.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
BASE COUNT          339 a          320 c          299 g          294 t
ORIGIN      
        1 ccgcctatgg cgggccgttt tgtatggaaa ccagacccta tgttcaaaac gacgctctgc
       61 accttattaa ttaccgcctc ttgctccaca tttgccgccc ctcaacaaat caacgatatt
      121 gtgcatcgca caattacccc gcttatagag caacaaagga tccccggtat ggcggtagcg
      181 gtaatttatc agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag
      241 cagcccgtca cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc
      301 gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa
      361 tactggcctg aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc
      421 tacaccgctg gcggcctgcc attgcaggtg ccggatgagg tgaaatcctc aagcgacttg
      481 ctgcgcttct atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc
      541 aactccagta tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag
      601 caggcgatga aaactcgtgt cttccagcca ctcaaactca atcatacgtg gattaatgta
      661 ccgtccgcag aagaaaagaa ctacgcctgg ggatatcgcg aaggtaaggc agtacatgtt
      721 tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt cgaccattga agatatggcc
      781 cgctgggtgc aaagcaattt aaaacccctt gatatcaatg agaaaatact tcaacaaggg
      841 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg
      901 gaaatgctgg actggccggt aaatcctgac atcatcatta acggcagtga caataaaatt
      961 gcactggcag cacgccccgt aaaaccgatt acgcccccaa ctcctgcagt acgcgcatca
     1021 tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa
     1081 aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagttacc
     1141 gccgcctggc agattctcaa cactctacag taaaatttca tcgggtccga attttcggac
     1201 cttttctccg cttttccttg ctgtcatcta cacttagaaa aaaaccagta ag
//
LOCUS       DAOFSV010000399         1059 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.351, whole genome shotgun sequence.
ACCESSION   DAOFSV010000399 DAOFSV010000000
VERSION     DAOFSV010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.351"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            102..560
                     /gene="gpt"
                     /locus_tag="QBH27_004974"
     CDS             102..560
                     /gene="gpt"
                     /locus_tag="QBH27_004974"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDQ7017147.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            652..>1059
                     /locus_tag="QBH27_004975"
     CDS             652..>1059
                     /locus_tag="QBH27_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ7017148.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAA"
BASE COUNT          250 a          273 c          273 g          263 t
ORIGIN      
        1 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
       61 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
      121 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
      181 aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggt gcgttactgg
      241 cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca
      301 accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta
      361 ttgatgacct ggtagatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag
      421 cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gattatgttg
      481 ttgatatccc gcaaaatacc tggatcgaac agccgtggga tatgggcgtc gtattcgtcc
      541 cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta
      601 acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc tatgacacag
      661 gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta
      721 gtccgccgct ttagtcacgg cgcacaactg cctgtgcagt cggcccttga tggtaaaacc
      781 atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgacccc
      841 cgtgaaatcc tcgaagtcca ggcgcgtatt gtgatgagcg atgccgaacg taccgacgat
      901 gatttatacg atacggtgat tggctaccgc ggcggcaact ggatttatga gtgggctacc
      961 caggcgatgg tgtggcaaca aaaagcctgt gcggaagaag atccgcaact cagcggtcga
     1021 cactggctgc atgcggctac gttgtacaac attgctgcc
//
LOCUS       DAOFSV010000400         1042 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.352, whole genome shotgun sequence.
ACCESSION   DAOFSV010000400 DAOFSV010000000
VERSION     DAOFSV010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.352"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            complement(<1..337)
                     /gene="intI1"
                     /locus_tag="QBH27_004976"
     CDS             complement(<1..337)
                     /gene="intI1"
                     /locus_tag="QBH27_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDQ7017149.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRR"
     gene            495..968
                     /locus_tag="QBH27_004977"
     CDS             495..968
                     /locus_tag="QBH27_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HDQ7017150.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          286 a          228 c          254 g          274 t
ORIGIN      
        1 agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca
       61 ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct
      121 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
      181 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
      241 ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
      301 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
      361 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
      421 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttagcca
      481 ttaagggagt taaattgaaa atatcattga tttctgcagt gtcagaaaat ggcgtaatcg
      541 gtagtggtcc tgatatcccg tggtcagtaa aaggtgagca actactcttt aaagcgctca
      601 catataatca atggctcctt gtcggaagaa aaacatttga ctctatgggt gttcttccaa
      661 atcgcaaata tgcagtagtg tcaaagaacg gaatttcaag ctcaaatgaa aacgtcctag
      721 tttttccttc aatagaaaat gctttgaaag agctatcaaa agttacagat catgtatatg
      781 tctctggcgg gggtcaaatc tataatagcc ttattgaaaa agcagatata attcatttgt
      841 ctactgttca cgttgaagtc gaaggtgata tcaaattccc tataatgcct gagaatttca
      901 atttggtttt tgaacagttt tttatgtcta atataaatta tacataccag atttggaaaa
      961 aaggctaaca atgcgttgca gcaccagtcg cttcgctcct tggacagctt ttaagtcgcg
     1021 tctttgtggt tttgctgcgc aa
//
LOCUS       DAOFSV010000401         1010 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli strain MS6396 isolate MS6396
            SAMD00393898-rid17606623.guided.353, whole genome shotgun sequence.
ACCESSION   DAOFSV010000401 DAOFSV010000000
VERSION     DAOFSV010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00393898
            Sequence Read Archive: DRR310883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682805.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682805.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 22:55:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 30 of 130
            Pseudo Genes (multiple problems)  :: 31 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00393898-rid17606623.guided.353"
                     /strain="MS6396"
                     /isolate="MS6396"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan"
                     /collection_date="2013"
     gene            24..812
                     /locus_tag="QBH27_004978"
     CDS             24..812
                     /locus_tag="QBH27_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HDQ7017151.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
BASE COUNT          210 a          285 c          288 g          227 t
ORIGIN      
        1 cgctgaactt aacgttaggc atcatgggtg aatttttccc tgcacaagtt ttcaagcagc
       61 tgtcccacgc tcgcgcggtg atcgagcgcc atctggctgc gacactggac acaatccacc
      121 tgttcggatc tgcgatcgat ggagggctga agccggacag cgacatagac ttgctcgtga
      181 ccgtcagcgc cgcacctaac gattcgctcc ggcaggcgct aatgctcgat ttgctgaaag
      241 tctcatcacc gccaggcgat ggcggaacat ggcgaccgct ggagctaact gttgtcgctc
      301 gaagcgaagt agtgccttgg cgctatccgg cgcggcgtga gcttcagttc ggtgagtggc
      361 tccgccacga catcctttcc ggaacgttcg agcctgccgt tctggatcac gatcttgcga
      421 ttttgctgac caaggcgagg caacacagcc ttgcgcttct aggcccatcc gcagccacgt
      481 ttttcgagcc ggtgccgaag gagcatttct ccaaggcgct tttcgacact attgcccagt
      541 ggaatgcaga gtcggattgg aagggtgacg agcggaacgt cgttcttgct cttgctcgca
      601 tttggtacag cgcttcaact ggtctcattg ctcctaagga cgttgctgcc gcatgggtat
      661 cggagcgttt gcctgccgag catcggcccc tcatctgcaa ggcacgcgcg gcgtacctgg
      721 gtagcgagga cgacgaccta gcaatgcgcg tcgaagagac ggccgcgttc gttcgatatg
      781 ccaaagcaac gattgagaga atcttgcgtt gagcggcatg tgcgaaaagt gcatcgaccc
      841 gcgccgaggg catctgatgc ctaactcggc gttcaagcgg acgggctgcg cccgccgctc
      901 aactatgcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
      961 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
//
